LOCUS       DAOSGS010000001        40250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_763_length_40250_cov_6.217739, whole genome shotgun sequence.
ACCESSION   DAOSGS010000001 DAOSGS010000000
VERSION     DAOSGS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 40250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40250
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_763_length_40250_cov_6.217739"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLQ22_00005"
     CDS             complement(<1..182)
                     /locus_tag="PLQ22_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58376.1"
                     /translation="MHYTKRIYKNFPLHFAGYEQEPNFILYFINFNQEKIKEITLEFF
                     GKQQTYPINSSESKIT"
     gene            complement(184..519)
                     /locus_tag="PLQ22_00010"
     CDS             complement(184..519)
                     /locus_tag="PLQ22_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58377.1"
                     /translation="MNTNKTITQIGIILVLVIMPLAIGIPLFLANRDRPEFLEIPLAI
                     FGVFELLVLTVRIQVRDNKKRKAGNLKEDKGSEEYQSHVNFRKIILISAFINLALSLV
                     AFWIFGRGV"
     gene            complement(616..1557)
                     /locus_tag="PLQ22_00015"
     CDS             complement(616..1557)
                     /locus_tag="PLQ22_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH58378.1"
                     /translation="MRHEDSKDDREVLLEVQDVDIYFKVGKFTVKACNHLNFKVYKGE
                     TFGLVGESGSGKTTITRAIIGINELTHGRILFKGQDISRIKDKEGKKNLKKNIQMIFQ
                     DPTASLNERANIDYIVSEGLYNFNLFENNEDRHNKVVKAMREVGLLPEHLSRYPHEFS
                     GGQKQRIGIARALIVEPELVLADEPISSLDVSIRAQVLNLLRDLQEQNNLTYIFIAHD
                     LSIIKYISDRIAVMHQGYVVELGTAESIYSNPVHPYTKSLLTAIPQPDPASKDDRVKV
                     IYNKGNMDYEKMDWIEVVPEHFVLGTPELIKKWTKKK"
     gene            complement(1597..2061)
                     /locus_tag="PLQ22_00020"
                     /pseudo
     CDS             complement(1597..2061)
                     /locus_tag="PLQ22_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
     gene            complement(2153..3241)
                     /locus_tag="PLQ22_00025"
     CDS             complement(2153..3241)
                     /locus_tag="PLQ22_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58379.1"
                     /translation="MELKSPGQVQNGEKIIEIKHLEVRFSVRGKVLTAIRNVSLDIIK
                     GEIIAIVGESGSGKSVTTKTLNGMLEENGRVSDGVINYAGVDLAKFSSDVEWLNIRGK
                     RIAMIFQDPLTSLNPLRRIGSQIAEVIKIHRGLSKAEAKEEAISLLKRVGIPNAEKRY
                     TDYPFQFSGGMRQRVVIAIALACSPDILICDEPTTALDVTIQAQILDLIKELKDEYGF
                     TVIFITHDLGVVAEIADRVAVMYAGQIIELGTANDIFYNPQHPYTWALLSSLPQLGQK
                     GEKLYSVPGTPPSLFNRIHGDAFAPRNSFALKVDFVEEAPLFKVGGQHYAKTWLLDPR
                     SPKVERPKIISEIPARVAAIYGGGEKVE"
     gene            complement(3831..4835)
                     /locus_tag="PLQ22_00030"
     CDS             complement(3831..4835)
                     /locus_tag="PLQ22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58380.1"
                     /translation="MRNLDKFNVDNLPGFNGGEATFAQLPKKEELFTLVGEDKSLESE
                     RIATAPYSYFKSVFKVFFKNPGAIIGIVVLVLIVVGAIIIPFFTPEGFLAVNMHRVHL
                     KPDAVHLLGCDIEGKDLFYGIWTGIRKSLSLAVISAGINLVVGTIAGLIWGFFRKLDV
                     FFIGLYNLISNIPGLLIYMLLALVLRATFPTMQDETRLIISLTFTGWVGLSQFVRNQT
                     LIILNREYNLASHTLGTPARRIMTKNLLPYLLAVIVTQGSLMIPGIISSEVGLSFFGL
                     GLTPTKISLGSLIENGRKNFTLYFHEFIWPSLALGLIILAFFLIGFALSDSLDPRRHR
                     "
     gene            complement(4846..6072)
                     /locus_tag="PLQ22_00035"
     CDS             complement(4846..6072)
                     /locus_tag="PLQ22_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58381.1"
                     /translation="MILQAATHPVFTVFLTLVILWLIGSALTLIYVELSNEQKGKLKV
                     AAKRVKKALTNETFVYILKRIFSSLFTLLLVVVLVSLLLRLIPDYQLYDVPTYNKIKG
                     SNPSGAEAWRNLVLYRYGRTTLDGHRRSGLVGALQYIYWLLPIPKKIPVTWDTNYTRI
                     IRYWEGTVYLGKSIMYPDKEIPQIIRERMGISMRISLITTALVYVLSYPLGLVMAKKP
                     GGVIDKLGTAFIVLNYAIPGLVFYLLMNSVMGKADGIFGWLKAGFVYNENIPTSLIPP
                     IFCMVFLSIPGTSMWVRRFTVDELNSDYVKFAKSKGLSENRIMYTHVLRNSIVPLVRS
                     IPGTLLFSFVGSYYVEKIWSIPGTGGILTTALTRNDFQLIQGLTMIYAGLSMLAFLLG
                     DIITVFFDPRIRLRAR"
     gene            complement(6176..8539)
                     /locus_tag="PLQ22_00040"
     CDS             complement(6176..8539)
                     /locus_tag="PLQ22_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH58382.1"
                     /translation="MKKLTSLLLVLFAALLLVGCSCDPGGNGGDPEEQFVGGYGSLVD
                     KYKYEKNDAVEEGFISLASKYSNPPAQGKAGKDGYGKTYTVAGMTFDIKNYYKGAYST
                     DTDQEHFDYLVNSWTYNSEKYTNMVEGLIDNDKHGALVGVMAKGYKIEDADGGAKEIY
                     TFQLHEGVQWVKNDTGEAYAEVTADDFVAGLKYVLNPKVASATVQIVTGLIDGAAEYY
                     EAHTVKNSVDSYNADSAAWIASWKAANGSEENPATDEQATAAHDAEVARLAELEAHLD
                     FSTVGVKANGKYKVSYKLIEKVPYFLSNLTYSPFLPVHQPFLDEVGTDFGISENKILV
                     NGAYRMTRYDRESKIEYTKNTKYFDVDHVYIDHIELQYYSADIATNSKLREWFESGAV
                     DSFTVNENDLVGYEKYVLGPNGTGSLEEPANPLCNAVPSVGGATYIGYFNFNRKNYEY
                     PADIPAKTAAEQNATKLAVRNTNFRLGVQYGQDALKYLTYYNPNFPSQWLMRGYTVRE
                     LATDENGKDYADHVDEVWREKQGISGEASLTGFLNGKGSVDPTYSPTKAAEYFAAAKE
                     ELMAAGLTEADFPIKLDVIGTRNATVRPYQTATYNSITENSGGVVQVQILVPADANQN
                     VQWGSILSNYDYSLWSGWGPDYADPKTFLHTMVVGGDMVEMLGFDAESLKEAAIAQLQ
                     QEILGPYTALYELGNAITDSSRMSERFRKFAEAEYALIYEYAIIVPWLSQNGYRPAVA
                     RTIPYQAGRASYGLTSDKFKNVVVTKGSGITQEQRAAIVAAYKAEVE"
     gene            8801..9799
                     /gene="trpS"
                     /locus_tag="PLQ22_00045"
     CDS             8801..9799
                     /gene="trpS"
                     /locus_tag="PLQ22_00045"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOH58383.1"
                     /translation="MEKRKIILSGVKPTGTPTLGNYIGAMRNFGKLQEQHKNDLFVFF
                     VADLHALTTPQDPKELKKNINDVAALYLACGLKEENLLLFIQSEVVEHVQLSLILEGM
                     TYMGELERMTQYKDKKQKQVEGVSSALFTYPVLMAADILLYDADLVPVGEDQMQHLEI
                     TRDLANRFNNRFGETFIVPEGLVVKTGARIKDLQNPLIKMDKSAPNPKGCIYLLEPIS
                     SVKKKIMSAVTDNLGEVKYNVKKQPGIANLMTIYSSLTNLSFKEIEKKYTGKGYGEFK
                     KDLAEIVAKEIEGVQKRYNELINSEYLKNLLEKGKIEASKIASKKINKVYKKVGLK"
     gene            complement(9821..11083)
                     /gene="tyrS"
                     /locus_tag="PLQ22_00050"
     CDS             complement(9821..11083)
                     /gene="tyrS"
                     /locus_tag="PLQ22_00050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH58384.1"
                     /translation="MDLISDLKWRGLIYDIVDEEGLVARLKEGPVSVYCGFDPTADSL
                     HIGHLVPIITLMRFQKYGNRPVAIIGGGTGLIGDPSGKTAERSMNTKETVALWSKCFE
                     KQIGHFLKFDGKTSFCVNNYDWFSELKAIDMWRDYGKYFNINIMMQKDSVKSRLESGG
                     LTYLEFSYMIMQSIDFLKVYQDPKIKCEIQIGGQDQWGNLTAGLDLIRKIEGSEAKAY
                     ALTMPLILKSDGTKYGKTESGAVWLDKEKTTPYEFYQFFINVADADVMQLLKYYTFLS
                     HEEINALEKSLKEKPELREAQKTLAWEMTCLVHGQKATEQAIKVSEALFSGDVKELTK
                     EDIIMGFKGVRQEEIEEDNISLIDALVLLELAQSKREARQFIENGAILVNGDQEKDLD
                     FVVKKKNALDKAFTIIRRGKKRYGIIKH"
     gene            complement(11158..12174)
                     /locus_tag="PLQ22_00055"
     CDS             complement(11158..12174)
                     /locus_tag="PLQ22_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH58385.1"
                     /translation="MSQQIKIYDVAGAAGVSLATVSRVLNHPEKVKAETRERVLKIIK
                     EKGYKPNANARGLASKRSTTVAIVIPSISRASVAEMIEGIYDSARKYGYTIRLFITKD
                     EEDPRDAWSEVIASSVDGILFMNDETTDTTYKEIAHTPVPVVFVNNISSSDEFGSISI
                     DYEECAYQITKEMIKRGNKKILFIDTIHKYSANELKAAGYERAMKEAGLEIDILYSTG
                     NININQNDFKTYLNKKVPEVALAVRDSMAISFMNVATKKGIKVPEQLQVIGFQNTRYA
                     VLSNPTLTCVAASIYDIGSRAMSYLTEMMKDDQKKLEERESYQKKAANIIVDYDIVWR
                     ESTK"
     gene            complement(12295..12648)
                     /gene="rplS"
                     /locus_tag="PLQ22_00060"
     CDS             complement(12295..12648)
                     /gene="rplS"
                     /locus_tag="PLQ22_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020008669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH58386.1"
                     /translation="MSQQLIADVTKEYLKTDIPEFRPGDTVRVHVKIKEGARERIQIF
                     EGLVIKRQGGGISETYTVRKISYGVGVERTFPVHSPNVAQIEVVRMGKVRRAKLHYIR
                     SLSAKAARIKERKKF"
     gene            complement(12697..13422)
                     /gene="trmD"
                     /locus_tag="PLQ22_00065"
     CDS             complement(12697..13422)
                     /gene="trmD"
                     /locus_tag="PLQ22_00065"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH58387.1"
                     /translation="MRIDVLTLFPEMFTGVVNSSILQRAIGENHLEINIIDYRQFSLD
                     KNHKVDDYPYGGGAGMVIEVEPIVLALRSLSKGYTILTSPKGATYNQNKAKELSKKDH
                     LIIICGHYEGIDNRITNYIDEEISIGDYILTGGEIAALAMIDSIARLIPNVLGNQESL
                     QSESFEELLEYPQYTRPEEFEGHKVPSVLLSGHHENVRKWRRFQSLKRTYERRQDLLE
                     KTQLSKEDLEFLKLIKEGKDLEF"
     gene            complement(13419..13910)
                     /gene="rimM"
                     /locus_tag="PLQ22_00070"
     CDS             complement(13419..13910)
                     /gene="rimM"
                     /locus_tag="PLQ22_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOH58388.1"
                     /translation="MELVIIGTIVNHHGLKGEVRVIAHTDYPKERFKKGKTFYVLKES
                     EYEKIIIDSVRFHKNFVLLKFNNINDINDCLEYKGLDIYSERFEDENIYITDLINFDA
                     YDEKDNLIGSVVDYLEVPQGYILELETKEGQKVLVPYVDEFIKEIDFENQEIIIHLIE
                     GML"
     gene            complement(13913..14164)
                     /locus_tag="PLQ22_00075"
     CDS             complement(13913..14164)
                     /locus_tag="PLQ22_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH58389.1"
                     /translation="MDVNFEEFLKNLILPLVEKPEEVVVKELETSSDDTVALQVLVAN
                     DDLGRVIGKNGRIINALRTILYSCGARYGKRIEVSVDSF"
     gene            complement(14167..14439)
                     /gene="rpsP"
                     /locus_tag="PLQ22_00080"
     CDS             complement(14167..14439)
                     /gene="rpsP"
                     /locus_tag="PLQ22_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH58390.1"
                     /translation="MVKLRLQRLGAKKRPYYRIVATESSNPRDGRFIEIIGTYHPIEK
                     DELKINEEKALKWLHNGAQPTDTVKSLFTKVGLIEKFTEEKKAKRA"
     gene            complement(14519..15223)
                     /locus_tag="PLQ22_00085"
     CDS             complement(14519..15223)
                     /locus_tag="PLQ22_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH58391.1"
                     /translation="MKLIKYVKEGLNLERKLAKVLIKTGQIEVNNKVILDPATNLKKD
                     DKISYLGELIDYREYEYIALYKKEGYVSSRNDEKAPSLLNLITDERLRKRLKIVGRLD
                     TDTSGLIFLTDDGDFIHKVTSPKKEIEKVYYVELEREITKEDILQLEKGVSIKEKNTE
                     YLTKPAKCKMLTETSILLAIVEGRHHQVKKMMRAVSNEVILLKREKIGPIDLEGLDPG
                     CYRYLTEKEIKYFKNN"
     gene            15315..15899
                     /gene="def"
                     /locus_tag="PLQ22_00090"
     CDS             15315..15899
                     /gene="def"
                     /locus_tag="PLQ22_00090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH58392.1"
                     /translation="MILYENILKEENKQDNEILRLKAKNVTLPLNEEDLETLSLIKEY
                     LDNSYNKEAIEKYALRPGVGLAAPQIGISKKIFGIKAYDENKVLHEYYVINPRIISES
                     VETTYLPTGEGCLSVDRDVQGLIFRPKKITVRTNLYDFEAKELKQVILKLKDYIAVVF
                     QHEYDHLQGILFIDRINEQDPFGIPENSSPVKMD"
     gene            15918..17609
                     /locus_tag="PLQ22_00095"
     CDS             15918..17609
                     /locus_tag="PLQ22_00095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH58393.1"
                     /translation="MINRNNLPLKKNLYSKDIKVFALGGLGEVGKNLYCIEIADELLV
                     IDSGVLFSNENVGVDYIVPDFTYLKENEHRIIGLFITHGHEDHIGGIPYLLRTVKIPK
                     VYASGLAYELIKNKMKEFSGVDINLVEYRRDSVYVFNNFIISFFLVNHSIPDAFGMAI
                     KTKLGYIIHTGDFKFDFTPIGDAAEFEKITSYQKEGVLLLLSDSTGSEIEDFSLSEKA
                     IAESIRTIFETIEGRVIIATFASNAYRLQTMISASIEYGRKVVAFGRSMEKNINVAQQ
                     LKYIEAPKGTFIHAREFPHIPNNQITILCTGSQGEPFAALSRIADGTHKTIQIEPGDT
                     IIFSSSAIPGNELNIYDTINKLYKRGARVIVDSPLTDTHTTGHASKTEQEIMLALTKP
                     KYFVPIHGEYAMLKEHSKTAERVGVKEENIFILENGDVLTITKDGASVTDKITTGNIY
                     IDSKRNIVDPEIIKTRKHLSEEGLLSIVLSLDEKYKLRRELSIITRGFIYVKESDDLL
                     QKIEERVIKTLDNYVAVSSKYNKKDLSALLVSTLSTYIFELTERKPLIIPVIIEQ"
     gene            complement(17624..18478)
                     /locus_tag="PLQ22_00100"
     CDS             complement(17624..18478)
                     /locus_tag="PLQ22_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58394.1"
                     /translation="MEIRGRVIDTIPSLRIIKVETRDRMYYVYLSRKMFRDFGPYFYE
                     TPYASMEVDKKEIHGKYACYHLKSFTKIIKHIRRERKVFFDIVEIQDSIKDIINRKEN
                     MLFLDLEYSLPSSRGYREKFIEIIQYGLVLYDKEGKKILEDQSLVKPNKKSSLNKQTL
                     NFVNREFKEFKDGVDYIVFYQTFEQLLEKYNPRIVVWGNNDVPTLEKSFRINHLRPLN
                     IRKRSLNLMQLIKNYYGIKNDLGLFETYELATKTKLEPQTHDAFEDAVILQGVYDLFK
                     KDINKKRI"
     gene            complement(18469..20217)
                     /gene="uvrC"
                     /locus_tag="PLQ22_00105"
     CDS             complement(18469..20217)
                     /gene="uvrC"
                     /locus_tag="PLQ22_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH58395.1"
                     /translation="MDLNSKLNLIPHKPGCYLMKDKMNEVIYVGKAKNLYNRVRSYFT
                     GINNLKNTKLVENIADIEYIITSNETEAFLLEISLIKKYDPKYNIALTDDKTYPYILV
                     TEEKHPRILYTRNLNYKGKFYGPYPNAASAREVADILNAIFPLRKCKKLPKEVCLYYH
                     IGQCLAPCVNKDIKEDSYTTYKKEIDSILTGNINQEVKTLKQKMNQASENLEYEKALS
                     YKKSIDALENLKIKQAVSIDIVDTDVFGYALEDGSLSIQVFHIRKGMIVERDGALLDV
                     LGNFEDVFSGYIDNFYFYQNNPLPKQILIPNLKLDFFNKELNKLVFIPKRGNKKDLLE
                     LACKNALEKLEFLKKKKESEYQKTIVAMNELSKLLGYDNLRTMEAFDNSNLLGTNPVS
                     AMVYFKDGNPVKDNYRKYKVKYVEGANDVATMQEVVYRRYLKLRDERKEYPDLIVIDG
                     GKAQVDGAVDILKQLRLSIPVIGLVKDKNHKTDRIYYQDKEITISKSGNLFKLLERVQ
                     EEVHRFAITFFRKTQRSNFLITSLDDIKGLSNKQKQEILKIIGDEDFRVKLDEMKLKE
                     DVELAVLDALEEETWK"
     gene            complement(20225..20563)
                     /gene="rbfA"
                     /locus_tag="PLQ22_00110"
     CDS             complement(20225..20563)
                     /gene="rbfA"
                     /locus_tag="PLQ22_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH58396.1"
                     /translation="MSYKQERLERQIERELGTILLGETKDQRLRYVTITKVRLNGDYS
                     LATVYYTIMGTEDQIENTKRILEEAKGFLRSTLSKKLKLRKTPELILQYDESMEYGER
                     IENILKDLKK"
     gene            complement(20553..22862)
                     /gene="infB"
                     /locus_tag="PLQ22_00115"
     CDS             complement(20553..22862)
                     /gene="infB"
                     /locus_tag="PLQ22_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH58397.1"
                     /translation="MVKGFTVREILEILEDNPKVTKEGLFDDLYNVIYTEKEITLDTI
                     VPEAICKNLGRKYGQEIKKTKKPKKETPKEEPKPEPKAEPKPVVEDKKEKVEEKPKVE
                     AKSEPKKEEPKQKVEVPKSKPEPKKETSKEEPQEEFEDEDEVELSKVYDGKYDEYEVE
                     AKTYTRLKNVKKKRKVTQRQSNVSGSKKADENVLIYTPGMTVAQIADGLTVSVGEIVK
                     KLVMLGYMYNATAALDKDLAELLAEDAGFILKEKILEDITKFEEMKIEDNPEDLVARS
                     PIITIMGHVDHGKTTLLDTIRNTRVTASEAGGITQHIGAYQVEKSGRKITFIDTPGHA
                     AFTEMRARGAQVTDIVVLVVAADDGIMPQTVEAIDHAKAAKVPIIVAINKMDKPQAHP
                     DQVLQELTKYGLVPEVWGGDTICVPISALKAQGIEELLEMILLTADMLELKANPNRLG
                     IGTVVEAKLDRGRGPVATLLVSNGTIKIGDPIVVGTTYGRIRSMTDEKSKLLTSAGPA
                     KAVEITGLSDVPEAGDHFMVFDDEKTARLVAEERNIRKTDAELNAGAVSLVSALSDDK
                     ELNLIIKGDVQGSIEALKSSLQKINIDGVKINIIRTGVGSVTETDIALASTSGSIIIG
                     FNIRPRANILDYAKEKNIEVRLYSVIYKLLEDIEEAMTGMLDPEYEEKVIGEAEVRNT
                     FKASKIGTIAGLYVTNGVIHNGDSIRLIRDSIVIYNGKIGSLRRFDDDVKEVREGFEC
                     GLTIKNFNDIKEGDLVETYRMVEVKRNVL"
     gene            complement(22862..23167)
                     /locus_tag="PLQ22_00120"
     CDS             complement(22862..23167)
                     /locus_tag="PLQ22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010010056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOH58398.1"
                     /translation="MGKISALQVLGLANRANELVLGYDQVLSSIQKNKVKLVLVASDA
                     SNKTKDTFSKKCFFYKIEILEVFTSEELSQALGRKAKIVGIKDQGFGELIKKRIEVK"
     gene            complement(23154..23432)
                     /locus_tag="PLQ22_00125"
     CDS             complement(23154..23432)
                     /locus_tag="PLQ22_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOH58399.1"
                     /translation="MKVRKEPIRKCLATGMRFSKKDLVRIVRTPEGEIKIDQTGKLNG
                     RGAYLSKSLEAIKLAKTKNIIGKALEVEIPEAIYEQLFQIVGDKDGKN"
     gene            complement(23441..24508)
                     /gene="nusA"
                     /locus_tag="PLQ22_00130"
     CDS             complement(23441..24508)
                     /gene="nusA"
                     /locus_tag="PLQ22_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH58400.1"
                     /translation="MISKSFFESLEEVAFDRGLAIDDVLKRVEIAMAGAAKDNGYSGE
                     VKIDLEEGKNRVRFFEYLYVIDGEEIPAEERTKGQITLEQAEAYTKSPKVGQTIKNEV
                     DFSKFSQRSAQKFKNMFISGLKELEREYAYRHFHSKIGEIVTATIISNQNDFIGFDLG
                     KNTISYMPIAEGLPEETYEVGESKKVYIIKVDQTTKGPKVYLSRKNKEIVRRLFEMNI
                     PEVKDGTVEIVNLSRDPGNRSKVGVLSKDPNVDPKGACVGNSGFRVKTINMALNGEKI
                     DIFTWKEDPKELVAEALLPAETVKVFVDEKEKSALAIVEDDQFSLAIGRNGQNVRLAA
                     YAIGWKIDIKTVSVAKTEGLL"
     gene            complement(24520..24981)
                     /locus_tag="PLQ22_00135"
     CDS             complement(24520..24981)
                     /locus_tag="PLQ22_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOH58401.1"
                     /translation="MKITKEIKDLCQKRAEELELEFKEVIFVRESGMSILRVIVDKKG
                     KLTTDDCSDLSMLISDDLDKLDIEIENYYLEVTSPGIERKLEFSELNEVLGSYVFVKL
                     YRKIQGEKEFYGDLVSYEEDKVTLKINFSGRFKNVEFEKKDIALIRMAIKF"
     gene            complement(25105..25845)
                     /locus_tag="PLQ22_00140"
     CDS             complement(25105..25845)
                     /locus_tag="PLQ22_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH58402.1"
                     /translation="MEEIRSVNNSYIKYLKSLKQKKYRKEHKMFLVEGYHLVEEAAKA
                     KVLDAVLVIDKKDQIQGVKNYLINSDIIEDLASTITPQNIIGVCSIKQKKELTSNKVL
                     VLDELQDPGNLGTVIRTAIAFGFTDIAVSNNTVDIYNDKVIRGTQGMLFRANFMEVEI
                     KDFLLDLKAKGYKVITTSLGEDCITPDQLPKLEKSALVLGNEARGLKDEVLELADFKV
                     TIPMADVVESLNVGVAGGIMMYLLSKQK"
     gene            complement(25846..26388)
                     /locus_tag="PLQ22_00145"
     CDS             complement(25846..26388)
                     /locus_tag="PLQ22_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58403.1"
                     /translation="MKSNENINQDLETRMREIDIKLEENAVKQEQYIMGYDNIDESEY
                     LALKEEEQELKAEKKAIKKTIKLSRPKKWYEEISVLAYVYAIIQIIVSSRLVMPYLTS
                     FLTDIIFPALGDQANTNFGAFVIILGPLLLSLLITIIIFLSMLPFKNKRKAWAVILVL
                     HTLSALTSVFVLIKIYNQVI"
     gene            complement(26378..27595)
                     /gene="thiI"
                     /locus_tag="PLQ22_00150"
     CDS             complement(26378..27595)
                     /gene="thiI"
                     /locus_tag="PLQ22_00150"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOH58404.1"
                     /translation="MYNIILVRYGEMTLKKANYKEFLKAINTNIKRKLKQFLKLQFSN
                     TDCRFYIHLNGEDYLEVIKVLDTISGLASYSPCLKVTSDLEEVAKQSISLIKEENLER
                     KTFKVETKRSNKTYPLTSQEITQKVAGLILKEIKLEVDVHHPDFVLNIELRNEETYIY
                     LRNYLGMGGLPAGTGGNGLLMMSGGIDSPVAGFLALRKGIALKAIHFASPPYTSNLSL
                     QKVIDLTKEISKYSEFEELELLVVPFTEIQIAIQKYCNPTYLITLMRRAMYKIAEQVA
                     LKEGMSIIVNGESVGQVASQTVESMMVVNEVTNFPVIRPLVTYDKQEIVDVAKKINTY
                     NVSILPYEDCCTVFVPKHPVIRPKVETAKKEEEKYEMNQLIKEAVSNIKRITLSYNEE
                     YFVGGEDKDKYEI"
     gene            complement(27588..28694)
                     /locus_tag="PLQ22_00155"
     CDS             complement(27588..28694)
                     /locus_tag="PLQ22_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOH58405.1"
                     /translation="MIYFDYAATTPVDKEVLETYIKANQNFFGNSTALHKLGIEAEYM
                     HQKAKQEMFALFKLEKYEAIYTMNATEANNIAIFGVVRKAKTGRIITTSIEHPSVKNV
                     MKELEKEGYDVVYLNVNEEGLISLNDLEKAMTKDTLLVSIIGVNNIVGSVQNIKEISK
                     IVKKYPKAKLHVDLVQAIGRLDIDFKDIDLFTFSSHKIYGLKGLGVLCFKNNIDLVPI
                     LYGSSVQLGLKPGTLDVPSIIANTKALKLLLQNQDKNYNYVRKLNLLLRKEIEHLPIL
                     INSPKNASPYILNISLPNLNGETIQRLLEEKGFIVGTGSSCSSKTKEVEPTLYAMFKD
                     EKRANNSVRISFSYHNKESDVLQLAKALKEIVNV"
     gene            complement(28774..29394)
                     /gene="rpsD"
                     /locus_tag="PLQ22_00160"
     CDS             complement(28774..29394)
                     /gene="rpsD"
                     /locus_tag="PLQ22_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOH58406.1"
                     /translation="MARYTGPVWKKSRRLGYSILETGKEFGRRNSGSFRRTYAPGQHG
                     QKRKKQSEYGLQLQEKQRVRFVYGLNEKQFRKTFDYAAKMEGQQGENFLKLLESRLDN
                     VVYRIGFASTRRQARQLVNHGHILVDGKKIDIPSYLVQPGQTVSLKEKSRSLDIIKEA
                     LEAMQSRKPYIEFDENNQAGKFARYPERDEFLTDINETLIVEFYNR"
     gene            complement(29628..30383)
                     /gene="tpiA"
                     /locus_tag="PLQ22_00165"
     CDS             complement(29628..30383)
                     /gene="tpiA"
                     /locus_tag="PLQ22_00165"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH58407.1"
                     /translation="MRKKIIAGNWKMNKTRDEALQFVYAVSEKLPHIDEVDSVVCAPF
                     PLLRTLCKRQGDNLRVGAQNMHYADNGAYTGEISADMLTTLGVAYVIVGHSERRAMFN
                     ETDETVNLKVKKAFEKGLIPILCVGETLEQREKGQEKQVVKAQLEKNLEGLNKNDIEH
                     LVIAYEPIWAIGTGRTASAEQAEEMCAYVREVVKDLYGQVADKMRVQYGGSVKVDNIK
                     EILSMPNIDGALVGGASLKADDFVKLVEAGVKK"
     gene            complement(30368..31600)
                     /locus_tag="PLQ22_00170"
     CDS             complement(30368..31600)
                     /locus_tag="PLQ22_00170"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH58408.1"
                     /translation="MSKKTVRDLDLKGKVVLMRVDFNVPLKGGVITNDNRIVQALSTI
                     KYVKENGGKLVLFSHLGRVKTEEDKKDSSLAPVVNRLSELLGEKVVFVPETRGKKLEA
                     AIKKLKEGEVLMFENTRFEDLDGNKESKNNPELGAYWASLGDVFVNDAFGTAHRAHAS
                     NVGVAANIKGGAVAGFLLEKEIKFIGDAVNVPERPFVAILGGAKVSDKISVIENLINK
                     ADKILICGAMAYTFFKALGYEVGKSKVEDDYVKYAEELLKKAKGKIILPVDHLVASEF
                     SADAEAELVDVHHTPADKMGLDIGPKTLEVFEKELHGAKTVVWNGPAGVFEFEKFAAG
                     TKGICEVLANLEGAKTIIGGGDSAAAAISMGYESKFTHISTGGGASLEYLEGKDLPGI
                     TCLNDSEKPKEKKKCAKK"
     gene            complement(31754..32110)
                     /locus_tag="PLQ22_00175"
                     /pseudo
     CDS             complement(31754..32110)
                     /locus_tag="PLQ22_00175"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
     gene            complement(32389..33510)
                     /gene="ugpC"
                     /locus_tag="PLQ22_00180"
     CDS             complement(32389..33510)
                     /gene="ugpC"
                     /locus_tag="PLQ22_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOH58409.1"
                     /translation="MATISFKNLNKVYDGNVHAVIDFNLEVKDKEFIVLVGPSGCGKT
                     TTLRMLAGLEDITSGELRIDDEIMNDVAPKDRNIAMVFQNYALYPHMTVYENMAFGLK
                     LRKFSRDEIDRKVQSAASMLGLSSYLDRRPKALSGGQRQRVALGRALVRDAKVFLMDE
                     PLSNLDAKLRVQMRAEIIKLTESLGVTTVYVTHDQIEAMTMASRIVVMKDGLVQQVGT
                     PKDIYDNPDNIFVGGFIGTPPMNFLEGYVSEKGQFVIGDHKKGFVLIEVPKDKFETLK
                     SQGFIEKPIVLGIRPENILDQPEDLKKHPHSIVKLKVDVAELLGAETQIYGVVAGQNL
                     VAKVEARIDLYGGDEVELALNMDRCHFFDPETEARIKKY"
     gene            complement(33580..34284)
                     /locus_tag="PLQ22_00185"
     CDS             complement(33580..34284)
                     /locus_tag="PLQ22_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH58410.1"
                     /translation="MWSQETIEKIKKRLLSLQFTDMKIKTMYEYLFLGGPFVDDDKYY
                     RFIIVSFKKGNHKPILKFFNELFHDSFILEIEDNYVLFYKEQQIDDIKNLMLSVMEDF
                     SLPFRVFVSSKVSSDDPKNFYLLYKAYCDFLNKDYYAFSSNKELIFETFQNSRAILKE
                     LRPIILNNCYNDHQIILIVEALINNDLNISQAANEIYLHRNTVNNKIDLVYQETQLNI
                     RHFTDVLAMYLLNLDK"
     gene            34377..35126
                     /locus_tag="PLQ22_00190"
     CDS             34377..35126
                     /locus_tag="PLQ22_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH58411.1"
                     /translation="MKLVSWNVNGLRACVKKGFFDFFKEMDADIFAIQETKMQEDQKE
                     FDVEGYYEYWFDAEKKGYSGTLIYTKQKPLNATIGIEGKYLDEGRLITLEYDNFYFIN
                     TYVPNSQDELKRLDYRMEYNDALKTYLTKLKKYKPVIICGDLNVAHQEIDIRNPDTNR
                     RNAGFTDEEREKMTELLDSGFIDTFRYLHPNKIQYSWWSYRFQARERNVGWRIDYFLI
                     SEDLKDKLQTAKIHDDIYGSDHCPVSITIKL"
     gene            35136..36377
                     /locus_tag="PLQ22_00195"
     CDS             35136..36377
                     /locus_tag="PLQ22_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOH58412.1"
                     /translation="MNNPKADFKIKKIEEEIILPYNIKYQKIKGATKTNYEGTVAGNK
                     MGEANISNEMIDANKYYSQNIVALTIPHNLDLKFKQVFKNKNSVWEREHLDILAQEYE
                     QETGEEVMAIINDDFFDIYRIFPFPGQPDGLVIQNQNNYKTKSYGNHMSGNLGVDLNN
                     NFVSFSLEESNNLFEKLPKIDILSSNRIKSFLINSINEKPKQGEIALYFGLPGDDQKV
                     NPKEIEGKGKLYSVKGLILPNGKNDFYGKGKITKSTNNILKEDIFFLDSLSEDFNKSI
                     KEGDLVRVQYNLKEEFKDYYAFFGYKGIVLKNGEFTKMDGNFKIRHPRTIIGIKENKD
                     VVLVVVDGRRPEQGSYGVNGLEMAAIMKRLGCVDAYNFDGGGSSALAIKEKNNFILKN
                     RPCEGPRKVINALIITKKVLE"
     gene            complement(36393..38120)
                     /locus_tag="PLQ22_00200"
     CDS             complement(36393..38120)
                     /locus_tag="PLQ22_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58413.1"
                     /translation="MFKKFVSYYKPHWKLFTADLIAASLMGAIDLIFPVASRKVLNEY
                     LNTDNIHMLYIIAAIMIGFFILRFFFSYFIGYYGHIMGIRIESDMRRDLFHKLQLMDY
                     QFYDKNKTGVLVTNLTTHLHDISEMSHHAPENLFIAGLMLVGSFIMLGLINWQLTLII
                     FLFMLLLVLFSLYRRKKMLERFAETRVVQGELAARVESSISGIRLTQAYSNREYEEAR
                     FENSNTQYKKARIKVFKELALFGTGNNFLINMANLAALVFGGLFVFKGHITIINLTAF
                     FLYINFLIQPINRLVNSMEQIQQGMSGFAQFYEVMQKEPVIVNPENGVVKEEFEGKIE
                     FKNVVFDYEEEDDDKKHLLNNFNLTIEAGKKIAVVGETGVGKSTLSKLIPRFYDVQDG
                     EILVDGINVKDYDIRSLRSAIGHVQQDVFIFYGTIKENILFGRPDANFEEVQEAAKKA
                     QIHDFIVSLDDGYDTIVGERGVRLSGGQQQRVSIARLFLKNPKILLLDEATSSLDNIT
                     EKQIQKALDLLALNKTTIIIAHRLSTIKNADEIIVLGKDGIMERGNHEELLKNKGYYF
                     DLYEASVMI"
     gene            complement(38177..38362)
                     /locus_tag="PLQ22_00205"
     CDS             complement(38177..38362)
                     /locus_tag="PLQ22_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010688392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOH58414.1"
                     /translation="MSANIYKENDILVFKKNHPCGGNSWKVLRVGVDYKLECTTCKRI
                     IIIPRYELNKMVKKTKE"
     gene            complement(38362..39201)
                     /locus_tag="PLQ22_00210"
     CDS             complement(38362..39201)
                     /locus_tag="PLQ22_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOH58415.1"
                     /translation="MFLSMFSEFWNEIKTFFTQTEVLNRLLYTAIAIVIAIIISLIFS
                     LIFKANVRANHPFRKRQITINKLVRGLAKAILGVMLVFVVFGIWGVDLGPMIAGAGVV
                     GLAISLGAQNAIRDLVAGISMILDNVLDVGDVVEIRDFKGFVEEVGIRSTKIKNFRGE
                     LATFNNGTIDKVINFSRNPTLAVVTATVAYGSDLDKVIKIFDQELETLVEQFPDVVTR
                     APKVMGVSNLGTHGVDLMITFEALPQQHFAAQRAVNKMIKEICDKHKIEIPLPQIVVT
                     NKK"
     gene            complement(39201..39968)
                     /locus_tag="PLQ22_00215"
     CDS             complement(39201..39968)
                     /locus_tag="PLQ22_00215"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOH58416.1"
                     /translation="MGQVITIANPKGGVGKTTTTINLGAGLGNLKQKVLLVDLDIKAN
                     LTTGLGIDRENIDKGIFDILLKKTNIKDSIVSFKRFDLIPNNCFHKQALSDNHYNFEE
                     LKKHLDIIKKEYDYVLIDTGPVMGVLLDMALYASDSVLIPTECQYFAYDGIKIMINKI
                     NAIQGLKTKIQENLVVEGILLTKFDNRNVFSYEMAENIKEIVANKVFKTVINYSSHLQ
                     EAPSRGKDVLSFSYNSIGSREYRLLAKEILERKENTE"
     gene            complement(39981..>40250)
                     /locus_tag="PLQ22_00220"
     CDS             complement(39981..>40250)
                     /locus_tag="PLQ22_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOH58417.1"
                     /translation="VSQMRIFLELAVPFLKVGGKVLLMKGKNYQIEVEESLFALKTLN
                     SEICDIIKYELPNNLGSRVIIEVLKKKKTSTLYPRTYNLIKGKPL"
BASE COUNT        13173 a         7196 c         5060 g        14821 t
ORIGIN      
        1 aaagtaatct tagattcact agaattaata ggataagttt gttgtttccc aaaaaactct
       61 aaagtaattt ctttaatttt ttcttgatta aaatttataa aatataaaat aaagttaggt
      121 tcttgttcat aacctgcaaa atgcaaagga aaatttttgt atattctttt tgtataatgc
      181 atactacacg cctctaccaa aaatccaaaa agcaaccaaa gataatgcta agtttataaa
      241 tgctgaaata agaataattt ttctaaagtt tacatgactt tgatattcct cactaccttt
      301 atcttcttta agatttccag cctttctttt tttattatca cggacttgta ttcttacagt
      361 aagtaccaaa agttcaaata ctccaaaaat agcaagagga atttccagaa attccggtct
      421 atctctattt gctaaaaata aaggaatacc tatagctaat ggcattatta caagtaccaa
      481 tataattcca atttgtgtta ttgttttatt tgtattcata ttctttacct cttatgtttt
      541 atattataca acatttcatc aaaatattaa aggaaaaaag cattcgaaaa cgaatgcttt
      601 tttataaact taggattatt ttttcttagt ccacttttta attagttctg gtgtacccaa
      661 aacgaagtgt tcaggaacta cttcaatcca atccattttt tcgtaatcca tattaccctt
      721 attataaata actttcacac gatcatcttt acttgctgga tctggttgag gaattgcagt
      781 taataatgat ttagtatatg ggtgaactgg gtttgaataa atagattccg ctgttccaag
      841 ttccacaaca tatccttgat gcattaccgc aattctatca gaaatgtact taataattga
      901 taagtcatga gcaataaaaa tatatgttaa gttattttgt tcttgtaagt ctcttaatag
      961 attcaatact tgagctctaa ttgaaacgtc aagtgaagaa ataggttcat cagctaaaac
     1021 taattctggt tcaacaatca aagctctagc aataccaatt ctttgttttt gaccaccact
     1081 aaactcatgt ggataacgag ataagtgttc aggtaataaa cctacttctc tcatagcttt
     1141 aactacttta ttatgtctat cttcattatt ttcaaataaa ttaaagttgt ataaaccttc
     1201 agaaacaata tagtcgatat ttgctctttc atttaaagaa gcagttggat cttggaaaat
     1261 catttgaatg tttttcttta gatttttctt tccttcttta tctttaatac gcgaaatatc
     1321 ttgaccttta aataaaattc taccatgtgt taattcgtta atacctataa ttgctctagt
     1381 aattgttgtt ttacccgaac ctgattcccc tactaagcca aatgtttccc ctttgtacac
     1441 tttgaaattt aagtggttac atgcttttac agtaaatttc cccactttga aatagatatc
     1501 aacatcttga acttctagta acacttcacg atcatctttt gagtcttcat gtcttataac
     1561 tttagctctt tctggaaact caattttaga aacttcctca tagaaatatg cttcagtcat
     1621 ttgaatataa ggaattaccc ataatagtaa tattccacca gttagtaagc ttaataaaag
     1681 ccaaccaata aaacttaagt ggagtttaaa taaacgtaag cgatatgaac catacataat
     1741 tgctttactc atattaatag atccattacc tgataattct ggacgatcaa ttactaaata
     1801 tggagctaag caatatgaat accacttaaa gattcctggg attacaagta atactgtcca
     1861 aaacgccaaa taaattgttt tgaatagccc taatagtaaa ttacgaacaa gattttcttt
     1921 tttaaatcct tcaaataaac tattaaattt aacttcttta ccacgaacta aatttaggta
     1981 ataattgcga tttccaacat taactggacc aaaacctaaa ataggaaaac tcaacaatgc
     2041 tgttacaaat gaaacggcca ttggctctcc aatgcctttt tcctttaatt tggctttggc
     2101 tttggctttc aataatgcag gttcaaatgg atttagaggt tttttctctt gtttactcaa
     2161 ctttttcacc tcctccataa attgcagcaa ctcttgctgg aatttcagaa ataatctttg
     2221 gtctttctac tttaggagat cttggatcta acaaccatgt tttcgcatag tgttggcctc
     2281 ctactttaaa tagcggagcc tcctctacaa aatcgacttt taatgcaaaa ctatttcttg
     2341 gcgcaaaagc atcaccatga atacggttaa ataaacttgg tggtgttccc ggaactgagt
     2401 acaacttttc tcctttttgc cctaattgag gtaatgaaga aagtaatgcc caagtataag
     2461 gatgttgcgg attatagaag atatcattag cagtaccaag ttcaataatt tgacctgcat
     2521 acataactgc tactctatct gcaatctcag caacaacacc taagtcgtga gtaataaata
     2581 ttacagtaaa accatattca tcttttaact ctttaattaa atctaggatt tgtgcttgaa
     2641 tagtaacgtc taaagctgta gtaggttcat cacaaattaa gatatctggt gaacaagcaa
     2701 gggcaatagc gataactact ctttgcctca tacctccaga aaattggaat ggataatccg
     2761 tatatctttt ttctgcatta ggtattccta ctctctttaa taaactaatt gcttcctctt
     2821 ttgcttcagc cttacttaat cctctatgaa ttttaataac ctctgcaatt tggctaccaa
     2881 ttcttcttaa aggatttagt gaagtaagtg gatcttggaa aatcatcgca attctcttac
     2941 cacgaatgtt taaccattca acgtctgatg aaaatttagc aaggtctacg cctgcataat
     3001 taataacacc atcagaaact cttccgtttt cttctagcat tccatttaat gtcttagtag
     3061 ttacagattt tcctgatcca gattcaccta caatcgcaat aatttcacct ttgataatat
     3121 ctaatgaaac atttctaatt gcagttaata ctttaccacg aacagagaaa cgaacttcta
     3181 agtgcttaat ttctataatt ttttcaccat tttgtacttg tcctggactt tttaattcaa
     3241 tctcactttt cttaagtttt ctacgttttt taaataattg attattatct aaaccacgtt
     3301 gacgatagaa taaatcttcc aaaacagcct tacaatattg gcaaacttct ttatcttttc
     3361 tatttggatc aagtttagta cgacagttag gacaatatgt cttactttgt tctttaataa
     3421 cgtaaccatc actaaaaatg atactatctt ctgtaaactt tacaaaactt tcttcctctt
     3481 ctttactctc tactactaag taaacacaac ttgttagtcg ttgttgtaac aatttttgac
     3541 aaggtaagtc tggtcgatct aaatatttaa ataaatttac aacaacaatt aaatctgtat
     3601 aaatacgaag attttgaaga atttcatctc ccttataaag ttgtaaattt tcttttggat
     3661 cccaaacaat caaatcaatc ccatcaacag tcatttttac tggaatttgt ccacttgcaa
     3721 gtttgtagta ttctttataa atcttattag ttaacttagt attaaactca gtttctaaaa
     3781 actctaaatc cccaacaaaa taaatttctt tctttaaagc catattatac ctacctatgt
     3841 ctcctaggat ctagcgaatc tgataaggca aaacctatta agaaaaatgc taaaatgatg
     3901 agacctaaag ccaaagatgg ccagataaac tcatggaaat atagcgtgaa atttttcctt
     3961 ccattttcga tgagtgatcc taaagaaatt ttagttggcg ttaaaccaag accgaagaaa
     4021 cttaatccta cctcacttga aattattcct ggaatcatca atgatccttg agtaacgata
     4081 acagctagta aatatggaag taagttctta gtcataattc tacgcgctgg agtacctagc
     4141 gtgtgagatg ctaagttata ctctctattt agaataatta gcgtttggtt acgaacaaac
     4201 tgagataaac ctacccaacc tgtaaatgtt aaagaaataa tcaatcttgt ctcatcttgc
     4261 attgttggga atgttgccct taacactaat gctaataaca tataaattaa taatccagga
     4321 atatttgaaa ttaagttata taacccaata aagaaaacat ctagttttct aaaaaagccc
     4381 caaatcaacc ctgcaattgt tcctacaaca aggttaatac cagctgaaat aacagctaat
     4441 gataaagact ttcttattcc tgtccatatt ccgtaaaata agtcttttcc ttctatatca
     4501 catcctaaaa gatgtactgc atctggtttt aaatgaactc tatgcatatt tacagctaaa
     4561 aacccttcag gtgtaaagaa aggaattatt atagctccaa caacaattag aactaaaacc
     4621 acgataccaa taatagctcc tggattttta aagaatactt taaacacaga cttaaaatat
     4681 gaataaggag ccgtagcgat tctttcagac tctaaacttt tatcttctcc aacaagagta
     4741 aataactctt ctttctttgg tagttgagca aaagtagctt caccgccatt aaaaccaggt
     4801 aaattatcta cattaaattt atctaaattt ctcatacgct acctcctatc ttgcccttaa
     4861 cctaattcta ggatcaaaga atactgtaat aatatcccct agtaagaatg ctaacataga
     4921 taaaccagca taaatcattg ttaatccttg gattaattgg aaatcatttc ttgttagagc
     4981 tgttgttaat attcctccag ttcctggaat tgaccaaatt ttttctacat agtacgaacc
     5041 aacaaacgag aataataacg ttcctggaat cgatcttact agtggaacta ttgagtttct
     5101 taatacatgt gtatacataa tacgattttc agataaacct ttagattttg caaattttac
     5161 ataatctgaa tttaattcat caaccgtgaa tcttcttacc cacattgatg ttcctggtat
     5221 tgataaaaac accatacaga atatcggtgg gattaaggaa gtaggtatat tttcgttata
     5281 aacaaatcct gcttttaacc aaccaaaaat tccgtctgct ttacccatta ctgagttcat
     5341 caataaatag aaaactaatc caggaatagc atagtttaat acgataaatg ctgttcctag
     5401 tttatcaatt acaccacctg gttttttagc cattacaaga cctagtggat atgataatac
     5461 ataaacgagt gccgtagtaa ttaaagaaat acgcattgaa atacccattc tttcacgaat
     5521 gatttgaggg atttctttat ctgggtacat tatactttta cctaaataaa ctgtgccttc
     5581 ccaatatctt atgattctag tgtaattagt atcccatgta actggaattt tcttaggaat
     5641 tggtaaaagc cagtaaatgt actgaagtgc cccaactaaa cctgatctac gatgaccgtc
     5701 taatgtagtt ctaccatatc tataaagtac taagtttctc catgcttctg caccagaagg
     5761 attagatcct ttgattttgt tataagttgg aacatcatac aattgataat ctggaattaa
     5821 tcttaataat aatgaaacta atactactac tagtaaaaga gtaaatagag aagaaaaaat
     5881 tctttttaaa atgtacacaa aagtttcgtt agtaagtgct tttttaactc ttttagctgc
     5941 taccttaagt tttccttttt gctcattact taattctaca tatattaaag ttaaagcact
     6001 tcctataagc cataaaatca ccaatgttaa aaatactgta aacactggat gcgttgctgc
     6061 ttgtaaaatc ataatttttc ctccatttat aattaaaaca aagaggggtt acttacgaaa
     6121 taattaaccc cccttattta aaattattta aatagtatta taaactattt atatactact
     6181 ctacttcagc tttgtaagct gcaacgatag ctgctctttg ttcttgagta ataccactac
     6241 ccttagtaac aactacgttc ttgaacttat ctgaagttaa tccatagctt gctcttcctg
     6301 cttgataagg aattgtacga gcaactgctg gacgatatcc gttttgtgat aaccaaggaa
     6361 cgataatagc atattcataa attaaagcat attctgcttc agcgaatttg cggaatcttt
     6421 cgctcattct actgctatca gtaatagcat ttcctaattc atatagtgct gtataaggtc
     6481 ctaaaatttc ttgttgtaat tgtgcaattg ctgcttcttt taagctttca gcatcaaatc
     6541 ctaacatttc aaccatatct ccaccaacta ccattgtgtg taagaatgtc ttagggtctg
     6601 catagtctgg accccaacca ctccataatg agtagtcata gttagataag attgaacccc
     6661 attgtacgtt ttggtttgca tctgcaggaa ctagaatttg aacttgaaca actccgcctg
     6721 agttttcagt aattgaatta tatgtagctg tttgataagg acgaactgtt gcatttctag
     6781 taccaataac gtctagtttg attgggaaat cagcttctgt taagcctgct gccattaatt
     6841 cttccttagc tgctgcaaag tattcagctg ctttagttgg actatatgtt gggtctacag
     6901 aaccttttcc gttaaggaat cctgttaatg aagcttcacc agagattcct tgtttctctc
     6961 tccaaacttc atcaacgtga tctgcataat ccttaccatt ttcatcagta gctaattctc
     7021 taactgtata acctctcatt aaccattgtg atgggaagtt agggttataa tatgttaaat
     7081 attttaatgc atcttgtcca tattgaacac ctaaacggaa gttagtattt ctaactgcta
     7141 atttagtagc attttgttca gctgctgttt tagctgggat atcagctgga tattcataat
     7201 tctttctatt aaagttgaag tatccaatat atgtagctcc acctactgaa ggaacagcat
     7261 tacataatgg gttagctggt tcttctaatg atcctgttcc atttggtcca agaacatatt
     7321 tttcataacc tacaaggtca ttttcgttta ctgtgaaact atcaacagca ccactttcga
     7381 accattctct taatttagag tttgtagcga tgtctgctga gtaatattgt aattcgatgt
     7441 ggtcaatata tacatggtct acatcaaaat attttgtgtt cttagtatat tcaatcttag
     7501 attctctatc atatctagtc attctataag cgccgttaac taaaattttg ttttcgctaa
     7561 taccaaaatc agtaccaact tcatctaaga atggttggtg aactggtaag aatggagaat
     7621 atgttaagtt agataagaaa taaggaacct tttcaataag tttgtaagaa accttatatt
     7681 ttccattagc tttaactcca actgtactaa agtctaagtg tgcttctaac tcagctaatc
     7741 tagcaacttc tgcatcatgt gcagctgtag cttgttcatc tgtagctggg ttttcttctg
     7801 agccattagc tgctttccaa gatgcaatcc atgcagctga atctgcgttg taactatcaa
     7861 cactattttt aactgtgtgt gcttcataat attcagcagc accatcaatt aggccagtaa
     7921 cgatttgaac tgtagctgat gctactttag gatttaaaac atattttaat cctgcaacaa
     7981 agtcatcagc agtaacttct gcataagctt cgcctgtatc gttctttacc cattgaactc
     8041 cttcgtgaag ttggaaagta taaatttcct tagctccacc atctgcgtct tcaatcttat
     8101 aaccttttgc cattactcca actaatgcac catgtttgtc gttgtcaatt aaaccttcaa
     8161 ccatgttagt gtatttttca gagttatacg tccaagagtt aactaaatag tcaaagtgct
     8221 cttgatctgt atctgtagaa taagcacctt tatagtagtt tttaatatcg aacgtcattc
     8281 ctgcaactgt ataagtcttg ccataaccat ctttacctgc ttttccttga gctggtggat
     8341 tagaatattt acttgctaaa gagatgaaac cctcttcaac tgcatcattt ttttcgtact
     8401 tgtacttgtc tactaaagac ccgtagccac ctacgaattg ttcttctgga tctccgccgt
     8461 ttccgcctgg atcacaagaa catccaacca acaatagagc agcaaataaa actaataata
     8521 aacttgttaa ttttttcatt ttttcctcct tgtttatttt atgtatcact ttgatgtgat
     8581 tgttactatt atacacacat ttatttacct tgtcaacatt tttttgaaat gtaagcgttt
     8641 tacactaaca gttagattaa acagttgcag aataacttgg tgtaaagcgc ttactttgtg
     8701 ttttaaatat cttgttttta atcttgcatt atgttatact tttataaaca atttattgaa
     8761 cttaagttgt ttatgaaatt aaaattataa ggaggttaca atggaaaaga gaaaaattat
     8821 cctatctggg gttaagccta cagggacacc taccttaggt aactatatag gagcaatgag
     8881 aaattttgga aagcttcaag aacagcacaa aaatgattta tttgtgtttt ttgtggcgga
     8941 cttacatgct ctaacaacac ctcaagatcc taaagaatta aaaaagaata ttaatgatgt
     9001 tgctgcttta tatttagctt gcggtttaaa agaagaaaac ttacttcttt ttattcaatc
     9061 tgaagttgta gaacacgttc aactatcttt aattttagaa ggtatgacat atatgggcga
     9121 acttgaaaga atgacgcaat ataaagataa aaagcaaaaa caagttgaag gagtatcttc
     9181 tgctttattt acttatccag tattaatggc tgcagatatc ttactatatg atgctgacct
     9241 tgttccagtt ggtgaagatc aaatgcaaca cttagaaatt acaagagatc ttgctaaccg
     9301 ttttaataat cgttttggag aaacatttat cgtacccgaa gggttagttg taaaaacagg
     9361 tgcaagaatt aaagacttgc aaaatccttt aatcaaaatg gataaatcag ctccaaatcc
     9421 taaaggttgt atttatttac ttgaacccat tagctcagta aagaaaaaaa taatgagcgc
     9481 agtaacagac aatttaggtg aggtaaaata taacgtaaaa aaacaacctg gtattgctaa
     9541 cttaatgact atttatagtt cattaactaa cctttctttt aaagaaattg aaaaaaaata
     9601 tactggtaaa ggttatggcg aattcaaaaa agacttagca gaaattgttg ctaaggaaat
     9661 tgaaggtgtt caaaaaagat ataatgaact aattaattca gaatatttga aaaatctact
     9721 agaaaaaggt aaaatagaag caagtaaaat tgcctctaaa aaaataaata aagtctacaa
     9781 aaaagtaggt ttaaaataaa aagagtcttg cgactctttt ttaatgtttt ataattccgt
     9841 atcttttttt accacgtcta ataattgtga atgctttatc taaagcattt ttctttttaa
     9901 ctacaaaatc taaatccttt tcttgatctc cgtttactaa gatagcgcca ttttcaataa
     9961 attggcgagc ttctcttttt gattgtgcaa gttctagtaa aactaaggca tcaatcaaag
    10021 agatgttatc ttcttcgatt tcttcttgtc ttacaccttt aaagcccatg atgatatctt
    10081 cttttgttag ttcctttaca tcaccactaa ataaagcttc acttacttta attgcttgtt
    10141 ctgtagcttt ttggccgtgg actaaacaag tcatttccca agctaaagtt ttttgagctt
    10201 ctcttagttc tggcttttct tttaagcttt tttctaaagc atttatttcc tcatgactta
    10261 agaaagtata gtattttaat aattgcataa catcggcatc agctacatta ataaagaatt
    10321 ggtagaattc ataaggtgta gttttttctt tatcaagcca aactgcacct gattcagttt
    10381 taccatattt agtaccatca ctctttaata tcaatggcat tgtaagggca taagctttag
    10441 cttcagatcc ttcaattttt ctaattaaat ctaaaccagc tgttaaatta ccccattgat
    10501 cttgtccacc aatttgaatt tcacatttaa tctttggatc ttggtaaact tttagaaagt
    10561 caattgattg cataatcata tagctaaact ctaaatatgt taatccgcca ctttctaatc
    10621 ttgatttaac tgaatctttt tgcatcataa tattaatatt aaaatattta ccataatctc
    10681 gccacatatc aatagccttt agttcagaaa accaatcata gttgttaaca caaaaactag
    10741 ttttaccatc aaactttaaa aagtgtccta tttgtttttc aaaacattta gaccatagag
    10801 caacagtttc tttcgtgttc attgatcttt ctgctgtctt gcctgatggg tcaccaatta
    10861 atcctgttcc cccaccaata attgctactg gtctatttcc gtatttttgg aagcgcatca
    10921 aagtaattat tggaactaaa tgaccgatat gtaaactgtc agctgttgga tcaaacccac
    10981 aatatactga aactggtcct tcctttagtc ttgctactaa gccttcttca tctacgatat
    11041 catatattaa tccacgccat tttaaatcac taattaaatc cactcttttc ctccttaata
    11101 aaagtcctta aaatatttta aggacgtttt ttatttttaa ccttaattct taaattccta
    11161 tttagttgat tcacgccata ctatatcata atctacgatg atattagctg ctttcttttg
    11221 ataactttct ctttcttcta gtttcttttg atcatctttc atcatttcgg ttaaatagct
    11281 cattgctctg ctaccaatat cataaattga tgctgcaaca caagtaagtg ttggattaga
    11341 aagaactgca taacgagtat tttgaaagcc aattacttgt aactgttcgg gaactttaat
    11401 tcctttttta gtagctacgt tcataaatga aatcgccatt gaatcacgaa ctgctaaagc
    11461 aacttctgga actttcttat ttaaataagt cttgaaatca ttttggttaa tatttatgtt
    11521 gcctgtagag tatagaatat ctatttcaag acctgcttct ttcattgctc tttcataacc
    11581 tgctgcttta agttcattcg cagaatattt atgaatagta tcaataaata aaatcttttt
    11641 atttccgcgt ttaatcattt ctttagtaat ttggtaagca cattcttcat aatcaattga
    11701 aatactacca aactcatctg aactagaaat attgtttaca aatacaacag gtactggggt
    11761 atgggcaatt tctttatatg tagtatctgt tgtttcatca ttcataaata gaataccatc
    11821 tacacttgat gcaataactt ctgaccaagc atctcttgga tcttcttcat ctttagtaat
    11881 aaataaacga atagtatagc catatttgcg agcactatca tagatacctt caatcatctc
    11941 agcaacacta gcacgactaa tcgaagggat tacaatcgct actgttgttg accttttact
    12001 tgctaagcca cgggcattag catttggttt ataacctttt tccttaatta tttttagtac
    12061 tctttctcta gtttctgctt ttactttttc tggatgattc aaaacacgtg atacagttgc
    12121 taaacttaca cctgcagcgc ctgccacgtc ataaatttta atttgttgac tcatatttat
    12181 ttacctcact ttcttcactt tcttttcata tgtattataa cacattgtga aaatattttc
    12241 aatcgttttc tttttatttt cagtaaataa aaagacaggt ttacctgtct tttattagaa
    12301 cttcttacgt tctttaattc tagctgcctt agcagataaa gatctaatgt aatgaagttt
    12361 agctctacgt actttaccca tacgaactac ttcaatttga gcgacattcg gactatgaac
    12421 agggaaagtt ctttcaactc ctacaccgta agatatctta cgaactgtat aagtttcact
    12481 aattcctcca ccttgtcttt taattacaag tccttcaaag atttggattc tttcacgagc
    12541 accctcttta atcttaacgt gtacgcgaac tgtatctcca ggtctaaatt cgggaatgtc
    12601 ggtttttaaa tattctttag tcacatcagc tattaattgt tgactcattt tctttcactc
    12661 tcctttgatt tacttttatt taaagtactt cttttactaa aactctaaat cttttccttc
    12721 tttaatcagt tttaaaaact ctaggtcttc tttagataat tgagtttttt ctaaaagatc
    12781 ttgtcttctt tcataagttc tttttaaact ttgaaatcta cgccattttc tcacattttc
    12841 atgatgacca gataataata cactcggaac tttatgtcct tcaaactctt ctggtcttgt
    12901 atattgagga tattctaata attcctcaaa actttcgctt tgtaatgatt cttggtttcc
    12961 taaaacatta gggattaatc ttgcaatact atcaatcatc gcaagtgctg caatttctcc
    13021 tccagttagg atataatctc caattgagat ttcttcatca atgtaattag taatacgatt
    13081 atcaatacct tcataatgac cacatataat aattaagtgg tcttttttgg ataattcttt
    13141 agctttgttt tgattgtaag ttgccccttt tggagatgtt agaatagtgt atccttttga
    13201 aagtgagcga agagctaaaa caattggctc aacttcaata accataccgg ctccaccacc
    13261 ataaggataa tcatctactt tatgattctt atctaaagag aactgacgat aatcaattat
    13321 attgatttct aaatgatttt cacctattgc tctttgtaag atagaactat ttacaactcc
    13381 tgtaaacatt tcaggaaata atgttaatac atctattctc ataacatacc ctcaattaaa
    13441 tgaattatta tttcttgatt ttcaaaatca atttccttaa taaattcatc tacataagga
    13501 actaaaactt tttgtccttc ttttgtttct aactctaaaa tatatccttg aggaacttct
    13561 aaataatcta caacgctgcc tattaaatta tccttttcat cataagcatc aaagttaatt
    13621 aagtcagtaa tatatatatt ttcatcttca aatctttcag aatagatatc taaaccttta
    13681 tattctaaac aatcattaat atcattaatg ttattaaatt ttaataaaac aaagttttta
    13741 tgaaaacgaa ccgagtcaat aattattttc tcatattcac tttcttttaa aacataaaaa
    13801 gtttttcctt ttttaaatct ttctttcgga taatcggtat gagctattac tctaacctca
    13861 ccttttaaac catgatgatt aactattgtt ccgataatta ctaattccat aattaaaacg
    13921 aatctacaga aacttcaatt ctttttccgt agcgagcacc acatgaataa agaatagttc
    13981 ttaatgcatt aataatacgt ccgtttttac caataacacg accaagatca tcatttgcta
    14041 ctagtacttg caaagcaaca gtatcatctg aacttgtttc tagttctttc acaactactt
    14101 cttctggttt ttccacaagt ggtaaaataa gattttttaa aaactcttca aaattaacat
    14161 ccacggctat gccctcttag ctttcttttc ttcagtaaac ttttcaataa gtcctacttt
    14221 agtgaacagc gacttaactg tgtctgtcgg ttgtgcacca ttgtgaagcc actttaaagc
    14281 tttttcttcg ttaattttaa gttcatcttt ttcgatagga tgataagttc ctataatctc
    14341 gataaatcta ccatctctag gattcgaaga ttcagttgct acaatacgat aatacggtct
    14401 ctttttagca cctaatcttt gtagtcttaa tttaaccata tatacctcca taactagata
    14461 tgattatacc ataattaaaa aaggctgtca agtaaaaaaa cttaacagca taatattttt
    14521 aattgttttt aaaatattta atctcttttt cagttaaata acgataacaa ccaggatcaa
    14581 gcccttctaa atcaatagga cctatctttt ctcttttcaa taaaattact tcgttactca
    14641 cagctctcat cattttcttt acttgatgat gtcttccctc tacaatcgca agcaaaatac
    14701 ttgtttcagt tagcatctta cacttagctg gtttcgtaag atattcggta tttttttctt
    14761 taattgaaac tcctttttct aattgcaaga tatcttcttt tgtaatttcc ctttctaact
    14821 caacataata aaccttttca atttcctttt taggtgaagt tactttatga ataaaatctc
    14881 catcatcggt taaaaagatt aaaccagaag tatcagtatc taatctacct acaattttta
    14941 atctttttct taaccgttca tcagtaatca agttcaaaag agaaggcgct ttttcatcat
    15001 ttctacttga aacataacct tcctttttat acaaagcaat atattcatat tccctataat
    15061 ctatcaattc acctaagtaa gatatcttat cgtctttttt taagtttgtt gcagggtcta
    15121 agattacttt attattaact tcaatttggc ctgttttaat taaaactttt gcaagttttc
    15181 tttcaagatt aagcccttct tttacatact taattaactt catagcatta atattataac
    15241 acaaataatc ttaatcaaac atactttgtt tgcttttata ttatagtttt ggtaaaatag
    15301 ataaaggtga taaaatgatt ttatatgaaa atattttaaa agaagaaaat aaacaagata
    15361 atgaaatctt aagattaaaa gctaaaaatg ttactttacc tttaaatgaa gaagatttag
    15421 aaactttaag tttaattaaa gaatatttag ataatagtta taataaagag gctattgaga
    15481 aatatgcttt gcgtcctgga gtaggcttag ccgcccccca aattggcatt tcgaaaaaaa
    15541 tctttggaat taaagcttat gatgaaaata aagttcttca tgaatattat gtaattaatc
    15601 caagaattat tagtgaatcg gtagaaacta catatctacc aactggtgaa ggctgtctat
    15661 ctgttgatag agatgtgcaa ggactaatct ttagaccaaa gaaaataact gttagaacta
    15721 atctttatga ttttgaagca aaagaactta aacaagtaat attaaaatta aaagattata
    15781 ttgcggttgt atttcaacac gaatatgatc acttacaagg aatcttattt attgatagaa
    15841 tcaacgaaca agatcctttt ggtatacctg aaaattcaag tccagtaaaa atggactaat
    15901 ttatgaaagg aggcaaaatg ataaatagaa acaatttgcc attaaagaaa aatctctata
    15961 gtaaagatat caaagttttc gctttaggtg gtttaggtga agtaggaaaa aacctatatt
    16021 gtattgaaat agctgatgaa ctattagtaa ttgattctgg tgttttattt tccaatgaaa
    16081 atgttggggt tgattatatc gttcccgact ttacttactt aaaagaaaac gaacatagaa
    16141 ttattggctt atttattact catggtcatg aagaccatat tggaggaatt ccttatctat
    16201 taagaactgt taagattccc aaagtttatg cctctggttt agcttatgag cttataaaaa
    16261 ataagatgaa agaatttagt ggtgtagata tcaacttggt agaatataga cgtgattctg
    16321 tttatgtttt taataacttt attatttctt tctttctagt gaatcacagt attcctgatg
    16381 cttttggcat ggcaattaaa actaaattag gttatatcat ccatacaggc gattttaaat
    16441 ttgactttac tccgattggt gatgcagcgg aatttgaaaa aattacttct tatcaaaaag
    16501 aaggtgtttt attgttactt tctgatagta ctggttcaga aattgaagac ttttctttat
    16561 cagaaaaggc aatagcagaa agtataagaa ctatctttga aacaattgaa gggagagtta
    16621 ttattgctac ctttgcctca aacgcatata gattacaaac aatgatttct gcttcaattg
    16681 aatacggaag aaaagttgta gcttttggta gaagtatgga gaaaaatatc aacgttgctc
    16741 aacagttaaa atatatagaa gctccaaaag gaacatttat tcacgcaaga gagtttcctc
    16801 atattccaaa taatcaaata acaattttat gtactggatc tcaaggagaa ccttttgcgg
    16861 cattatcaag aatagctgat ggtactcata aaactatcca aattgaacca ggagatacta
    16921 ttattttctc atcaagtgca attccaggaa atgaacttaa tatttatgat actattaata
    16981 aactatataa aagaggggca agggttattg tagatagtcc tttaacagac acccacacta
    17041 ctggtcatgc tagtaaaact gagcaagaaa ttatgcttgc ccttacaaaa ccaaaatact
    17101 ttgttcctat ccatggtgaa tatgcgatgc ttaaagaaca tagcaaaaca gctgaaagag
    17161 ttggagttaa agaagaaaat atctttatat tagaaaatgg cgatgtccta acgattacta
    17221 aagatggggc aagtgttaca gataaaatta caactggtaa tatttatatt gatagtaaaa
    17281 gaaatattgt tgatccagaa atcattaaga caagaaaaca tctctcagaa gaaggattgt
    17341 tatctatcgt tcttagttta gatgaaaaat ataaactaag aagagaacta tcaataatta
    17401 ctagaggttt tatttatgtt aaagagtctg atgatttatt acaaaaaatt gaagaacgtg
    17461 ttataaaaac tttagataat tatgttgctg tttcatcaaa atataataaa aaagatttaa
    17521 gtgctttgct tgtttcaaca ttaagtactt atatctttga attaacagaa agaaaaccat
    17581 taattattcc cgtaattatt gaacaataaa aaagaggatt attctaaatc ctctttttat
    17641 ttatatcttt tttaaataaa tcatagactc cttgtaagat aactgcatct tcaaaagcat
    17701 cgtgagtttg gggttctaat ttagttttag ttgcaagttc atatgtttca aataaaccta
    17761 aatcattttt tataccataa taatttttaa tcaattgcat taaattaaga cttctttttc
    17821 taatatttag cggtcttaaa tgatttattc taaaagattt ttctagtgta ggaacatcat
    17881 tattacccca aacaactatt ctaggattgt atttttccaa tagttgttca aaagtttgat
    17941 aaaaaactat ataatcaact ccatctttga attctttaaa ttctcgatta acaaaattta
    18001 aagtttgttt gtttaatgaa ctttttttat ttggttttac taacgattga tcttctaaaa
    18061 tctttttccc ttctttgtca tataaaacta atccgtattg aataatctcg ataaattttt
    18121 cgcgatatcc cctactacta ggtaaggaat attctaaatc taaaaacaac atattttctt
    18181 tacgattaat aatatcttta atagaatctt gaatctcaac aatatcaaaa aaaacttttc
    18241 tttctctacg aatatgctta ataattttcg taaatgattt taaatgatag catgcatatt
    18301 taccatgaat ttctttttta tcaacttcca tagacgcata tggggtttca taaaaatatg
    18361 gcccaaagtc tctaaacatt tttctcgaaa gatagacata atacattcta tctcttgttt
    18421 ctactttaat tattcttaaa ctaggaattg tatctataac tcttcctctt atttccatgt
    18481 ctcctcctcc aatgcatcta aaacagctaa ttctacatct tcttttaatt tcatttcatc
    18541 taattttact ctaaagtctt catcaccaat tatctttaga atttcttgtt tttgtttgtt
    18601 tgataaacct ttaatatcat ctaatgaagt tattaaaaag tttgatcttt gggtttttct
    18661 aaaaaaagta attgcaaaac gatgaacttc ttcttgaact ctttctaaaa gtttaaataa
    18721 gtttccactt ttactaattg ttatttcttt gtcctgataa taaatgcgat ctgttttatg
    18781 gtttttatct tttactaaac caattacagg aattgataat cttagttgtt ttaagatatc
    18841 tacagcacca tcaacttgag cttttccccc atcaattaca attaaatcag ggtactcttt
    18901 tctttcatct cttaatttta gatatctacg ataaacaact tcttgcattg tagcaacatc
    18961 gttagcaccc tctacatatt taaccttata ttttcggtag ttgtctttaa ctggattgcc
    19021 atctttaaaa taaaccattg cgcttacagg attagttcca agtaaatttg aattatcaaa
    19081 agcttccatt gttcttaaat tatcataacc taataattta gataattcat tcatcgcaac
    19141 aattgttttt tgatattcac tttctttttt cttctttaaa aactctaatt tttctaaagc
    19201 attcttacaa gcaagttcca ataagtcttt tttatttcct cgtttaggaa taaacactaa
    19261 tttgtttaat tctttattaa agaaatctaa ctttaaatta ggaattagaa tttgttttgg
    19321 taatggatta ttttgataga aataaaaatt atcaatataa ccgctaaaca cgtcttcaaa
    19381 attacctaaa acatcaagta aagctccatc tctttctaca atcattcctt tacgaatatg
    19441 aaagacttgg atacttaaag aaccatcttc taaagcatag ccaaatacat cagtatctac
    19501 aatatcaata cttactgctt gttttatttt tagattttct aaagcatcaa ttgacttttt
    19561 atagcttaat gctttttcat attctaaatt ttcactagct tgattcatct tttgctttaa
    19621 agttttaact tcttgattaa tattgccggt taaaatacta tcaatttcct ttttataagt
    19681 agtataacta tcttctttta tatctttatt aacacaaggg gcaagacatt gtcctatatg
    19741 gtaataaagg caaacttctt ttggtagttt cttgcacttt ctcaagggaa aaatagcatt
    19801 taagatatct gcaacttctc ttgcactagc ggcattaggg tatggcccgt aaaacttccc
    19861 cttataattt aagtttcttg tatataaaat acgtggatgt ttttcctcag taactaaaat
    19921 ataaggatac gttttatcat cagttaaagc gatattatat tttggatcat actttttaat
    19981 taagcttatt tctaatagaa atgcttccgt ttcatttgaa gtaattatat attcaatatc
    20041 ggcaatgttt tcaactagtt tagtattttt taaattatta ataccagtaa aataagaacg
    20101 aacacgatta tataaattct ttgccttccc cacataaata acttcattca ttttatcttt
    20161 cattagataa caacccggtt tatgagggat aagatttagc ttactattta aatccatagt
    20221 tcacctattt cttcaaatct tttaaaatgt tttctattct ttctccgtat tccatcgatt
    20281 cgtcatattg caagattaac tctggggttt ttctcaattt caatttttta cttaaagttg
    20341 atcttaagaa acctttagct tcttctaaaa ttctctttgt gttttcaatt tggtcttctg
    20401 ttcccataat cgtatagtaa acagtagcta aagaatagtc cccatttaat ctcacttttg
    20461 taatagttac atatcttaat ctttgatctt tagtttcacc aaggagaata gttccaagtt
    20521 ctctttcgat ttgtctttct aatctttctt gtttataaga catttcgttt tacctcaacc
    20581 attctatatg tttctactaa atcgccttct ttaatatcgt tgaagttttt aattgtaagt
    20641 ccgcattcaa agccttctct tacttctttt acatcatcat caaatcttct taaagagccg
    20701 atttttccat tataaataac aattgaatca cggattaaac gaatactatc accattatga
    20761 ataacaccat tagttacata taaaccagcg attgttccaa ttttagaagc tttgaaagta
    20821 tttctaacct ctgcttcacc gataactttt tcttcatatt ctggatctaa cataccagtc
    20881 atcgcttctt cgatatcttc caatagttta tagataacag agtataatct aacttcaata
    20941 tttttctctt ttgcgtaatc taaaatatta gcacgaggac gaatattaaa acctataata
    21001 attgaaccag atgtacttgc aagagcaata tccgtttctg taactgaccc aacaccagtt
    21061 ctaataatat taatcttaac tccatcaata ttaatttttt gtaatgaaga ttttaaagct
    21121 tcgattgagc cttgaacatc acctttaata attaagttta attctttgtc atcacttaaa
    21181 gcacttacca aactaactgc tcctgcattt aattcagcat ctgtcttacg aatatttctt
    21241 tcttctgcta ctaaacgagc agttttttca tcatcaaata ccataaagtg atcaccagct
    21301 tcaggtacat ctgataaacc agtaatttct actgctttag ctggtcctgc gctagttaac
    21361 aacttactct tctcatcagt cattgatcta atacgaccat aagtagttcc aacaacaatt
    21421 gggtctccaa tcttaatcgt accattactt acaagtaatg ttgcaactgg accacgacca
    21481 cgatcaagtt tagcctctac aactgtgcca attcctaaac gatttggatt tgcttttaat
    21541 tcaagcatat cggctgttaa taaaatcatt tctaataact cttcaattcc ttgagctttt
    21601 aaagcagaaa ttggaacaca aattgtatct ccaccccaaa cttctggaac taaaccatat
    21661 ttagttaatt cttgcaatac ttggtctgga tgagcttgag gcttatccat tttattaata
    21721 gctacaataa taggaacttt agctgcctta gcgtgatcaa ttgcttctac agtttgaggc
    21781 ataatgccat catctgcagc tacaactaaa acaacaatat ctgttacttg cgcaccacga
    21841 gctctcattt cagtaaaagc agcgtgacct ggagtatcaa taaatgtaat cttgcgacca
    21901 ctcttttcta cttgataagc accaatatgt tgtgtaattc cacctgcttc agatgctgta
    21961 actcttgtat ttctaattgt atctaataat gttgtttttc catggtcaac atgtcccata
    22021 attgtaataa taggacttct tgctactaaa tcttctggat tatcctcaat tttcatttct
    22081 tcgaatttag taatatcctc taagattttc tcttttaaaa taaaccctgc atcttcagct
    22141 aaaagttcag ctaaatcttt atctaaagct gcagttgcat tatacatata acctaacatt
    22201 accaatttct ttactatttc tccaacagaa acagttaaac catcagcaat ttgcgccaca
    22261 gtcataccag gtgtgtagat taaaacattc tcatcggctt tttttgaacc agaaacgtta
    22321 ctttgtcttt gcgttacttt tctttttttc ttaacgttct ttaatcttgt atatgttttt
    22381 gcttctactt catattcatc atatttacca tcataaactt tagaaagttc aacttcatct
    22441 tcatcttcaa actcttcttg tggttcttct tttgaagttt ctttctttgg ttctggtttt
    22501 gactttggaa cttcaacctt ttgttttggt tcttcttttt ttggttcaga cttagcttct
    22561 actttcggct tttcctcaac tttttctttt ttatcctcta caacaggttt tggttctgct
    22621 ttaggttctg gcttcggttc ttcttttgga gtttctttct taggcttctt tgttttctta
    22681 atttcttgac catattttct acctaagttc ttacatattg cttcaggaac tatcgtatct
    22741 aaagtaattt ctttttctgt atagattaca ttatataagt catcaaataa gccttctttg
    22801 gtaactttag gattatcctc aaggatttcc aaaatttctc taactgtgaa tcctttaacc
    22861 attatttcac ctcaattctt ttttttatta attctccaaa cccttggtct ttaataccaa
    22921 ctattttcgc ttttctacca agagcttgac ttagttcttc actagtaaat acttctaaaa
    22981 tctcaatttt ataaaagaaa cattttttag aaaaagtatc ttttgttttg tttgatgcat
    23041 cactagcaac tagaactaat tttactttat ttttctgaat acttgataaa acttggtcat
    23101 aacctaaaac taattcatta gctctatttg ctagacctag tacttgtaag gcactaattt
    23161 ttcccatcct tatcacctac aatttgaaac aactgttcat aaatagcttc tgggatttca
    23221 acttctaatg ctttaccaat tatatttttt gtttttgcta atttaatagc ttctaaagat
    23281 ttagaaagat aagcgccacg accattcaac tttcctgttt ggtcgatttt tatctctcct
    23341 tctggtgtcc taacaatacg gacaagatct tttttagaga atctcatccc tgttgctaga
    23401 catttacgga ttggctcttt tcttaccttc ataaaacctc ttacaataaa ccttccgttt
    23461 tagcaactga aacagttttg atatctattt tccaaccaat tgcataagca gctaaacgaa
    23521 cgttttgccc gtttctacca attgcaagtg aaaattgatc gtcttctaca atcgctagtg
    23581 ctgacttttc tttttcatct acgaaaactt taactgtttc agctggaagt aatgcttctg
    23641 ctactaattc ttttggatct tctttccatg tgaagatatc tattttctca ccatttaaag
    23701 ccatgttgat tgttttaact ctaaaaccag aatttccaac acatgctccc ttaggatcta
    23761 cgttaggatc tttacttaaa actcccactt tacttctatt gcctggatca cgacttaagt
    23821 taacaatctc tactgttcca tcttttactt ctggtatatt catctcgaat aatctacgaa
    23881 caatttcttt attctttctt gataaatata ctttaggacc tttagtagtt tggtctactt
    23941 taataatata aacctttttg ctttcgccaa cttcatatgt ttcttctggt aaaccttcag
    24001 ctattggcat atatgaaatt gtatttttac ctaaatcaaa tccaataaaa tcattttgat
    24061 tagagatgat tgtagcagta acaatttcac caatcttact atgaaaatga cgatatgcat
    24121 actctctttc taattcttta agtccactaa taaacatgtt tttaaacttt tgagctgaac
    24181 gttgagagaa tttagaaaag tcaacttcgt ttttaattgt ttgaccaact ttcggacttt
    24241 ttgtatatgc ttctgcttgt tctaaagtaa tttgtccttt ggttctttct tcagctggaa
    24301 tttcttctcc atcaattaca tataaatatt caaaaaatct aactctgttt tttccttctt
    24361 ctaaatctat cttaacttca ccactataac cattatcttt agcagcacca gccatcgcaa
    24421 tttccactct ttttaaaaca tcatcaatag ctaaaccacg atcaaatgct acttcttcta
    24481 acgattcaaa aaaactttta ctaatcattt atacccctcc taaaacttaa ttgccattct
    24541 tattaaggca atatcctttt tttcaaattc aacattctta aatcttccac taaagttaat
    24601 ctttaaagta actttgtctt cttcataact aactaaatcc ccataaaact ctttttctcc
    24661 ttgaatctta cgatatagct ttacaaaaac atatgaacca agtacttcat ttaattcgct
    24721 aaactctagt ttcctttcaa tacctggaga tgttacttct aaataataat tttcaatttc
    24781 gatatctaac ttatctaaat catcactaat taacatactt aagtcactac aatcatcagt
    24841 agttaactta cctttcttat caacaataac tcttaaaata ctcataccac tttctcttac
    24901 aaaaataact tctttaaatt caagttctaa ctcttctgct cttttttggc ataagtcttt
    24961 aatttctttt gtgattttca aaaaaacacc tccttcaaaa ttaaaggagc gagaaactca
    25021 ctccagtttt tattattata acaaatcatt tttaagatgt caaatgaata tcgaaaaaac
    25081 aacataattt aacaataaaa ttagttattt ttgcttactt aaaagataca tcattattcc
    25141 accagcaaca ccaacattca aactctcgac aacatcggcc atcggaatcg taactttaaa
    25201 atcagctaat tctaaaactt catcttttaa tccgcgtgct tcatttccta aaactaaagc
    25261 acttttttct agttttggaa gttgatctgg tgtaatacaa tcttcaccta aacttgttgt
    25321 aattacttta taaccttttg cttttaaatc taaaagaaaa tctttgattt ctacttccat
    25381 aaagttagcc ctaaacaaca tcccttgtgt tcccctaata actttgtcat tgtagatatc
    25441 tacagtgtta ttactaactg caatgtcggt gaaaccaaaa gctattgctg tcctaattac
    25501 agttcccaaa ttaccaggat cttgaagttc atctaatact aaaactttgt ttgatgttag
    25561 ttcctttttt tgctttatag aacaaactcc aataatattt tgaggtgtta tagtgcttgc
    25621 aagatcttca attatatcac tattaattaa ataattttta actccttgta tttggtcttt
    25681 cttatctatc accaaaaccg catctaaaac tttagcctta gctgcttctt ctactaaatg
    25741 atatccttca actaaaaaca ttttatgttc tttgcgatac tttttttgct ttaaagattt
    25801 taaatattta atataagaat tattaactga tctaatctct tccatttaaa tcacctgatt
    25861 atatattttt attaaaacaa aaactgaagt taaagcagat aaagtatgta aaactaaaat
    25921 aactgcccat gcttttcgtt tgtttttaaa tggtaacatt gataagaaaa ttataatagt
    25981 aattagtaga gataatagta atggccctaa aataattaca aaggcaccaa aattagtatt
    26041 tgcctgatcc cctaaagcag gaaaaataat atctgttaag aaagatgtta aataaggcat
    26101 aactaatcta ctagaaacaa taatttgaat aatagcataa acataagcta aaacagatat
    26161 ctcttcatac cattttttag gtcttgatag tttgattgtt tttttgattg ctttcttttc
    26221 tgcctttaat tcttgttctt cctcttttaa agcaagatac tctgattcat caatattatc
    26281 ataacccata atatattgtt cttgcttaac agcattttct tctaatttga tatctatttc
    26341 tctcattctt gtttctaaat cttgattaat attttcatta gatttcatat ttatctttat
    26401 cctcccctcc cacaaaatac tcttcattat aacttaaagt aattcgttta atattactta
    26461 ccgcttcttt tattaactgg ttcatttcat acttttcttc ttcttttttt gctgtttcta
    26521 ctttaggacg aattactggg tgtttaggta caaacactgt acaacaatcc tcatatggta
    26581 aaatcgaaac attgtaagta tttattttct tagcaacatc tacaatctct tgtttatcat
    26641 aagttactaa aggtctaata acaggaaaat tagtaacttc atttactacc atcatactct
    26701 caacagtttg acttgcaact tgacctacac tttccccatt tacaataata ctcattcctt
    26761 ctttcaaagc aacttgctca gctattttat acattgctct tctcattagg gtaatcaaat
    26821 atgtggggtt acaatacttt tgaattgcaa tttgtatttc tgtaaacgga actactaata
    26881 gttctaattc ttcaaattcg ctatatttag atatctcttt tgtaagatct ataacttttt
    26941 gtaatgatag attcgaagta tatggaggag atgcaaaatg aattgctttt aatgctattc
    27001 cttttcttaa tgcaagaaaa ccagcaacag gagaatcaat tcctccactc atcataagta
    27061 aaccatttcc accagtacca gcaggaaggc cacccattcc caaataatta cgcaaataga
    27121 tatatgtttc ttcatttctt aactcaatat ttaaaacaaa gtcaggatga tgaacatcta
    27181 cttctagttt aatttctttt aaaattaaac cagctacttt ttgtgtaatt tcctgtgatg
    27241 ttaagggata agttttattg cttcttttag tttcaacttt aaatgttttt ctttctaaat
    27301 tttcttcttt tattaaagaa atgctttgtt tagccacttc ttctaaatca gaagttacct
    27361 taaggcaagg actataacta gctagacccg agatagtgtc caacacttta ataacttcca
    27421 aataatcttc tccatttaaa tgaatataaa aacgacaatc agtattacta aactgaagtt
    27481 ttaaaaattg ctttagtttt cttttaatat tagtattaat tgcttttaaa aactccttgt
    27541 aattagcttt ttttaaagtc atttctccat atcttactaa aataatatta tacattgaca
    27601 atctccttta aagctttagc tagttgtaaa acatctgatt ctttattgtg ataagaaaaa
    27661 ctaattctaa ctgaattgtt tgctcttttt tcgtctttaa acattgcata taatgtaggt
    27721 tctacttctt tagttttaga agaacaacta gatcctgtcc caacaataaa tcctttttct
    27781 tctagtaatc tttgaattgt ttctccattt agattaggta aagaaatatt taaaatataa
    27841 ggactagcat ttttaggtga atttattaaa atcggtaaat gttctatttc ttttcttaat
    27901 aacaaattca attttcttac atagttataa tttttatctt ggttttgcaa taacaatttt
    27961 aatgctttag tattagcgat aatgcttgga acatctagcg tgcctggctt taatcctaat
    28021 tgaacactag atccatataa aatcggcact aagtcaatat tgtttttaaa acacaaaacc
    28081 cccaatcctt taagtccgta aattttatgt gaactaaaag taaataaatc aatatcttta
    28141 aaatcgatat ctaatctacc aattgcttga actaaatcta catgtaattt agctttagga
    28201 tattttttaa ctattttaga tatctctttt atattttgaa ctgaacctac aatattatta
    28261 acacctatga ttgatactaa taaagtatct ttagtcattg ccttttctaa atcatttaaa
    28321 gaaattaagc cttcttcatt tacatttaga taaacaacat cataaccttc tttttcaagc
    28381 tctttcataa cattttttac cgaaggatgt tcaatagaag ttgtgataat tcttcctgtt
    28441 tttgcttttc taacaacacc aaaaatagct atattattag cttcggttgc attcatagta
    28501 tagattgctt catatttttc tagtttaaat aaagcaaaca tttcttgctt tgctttttga
    28561 tgcatatatt ccgcctctat tcctagctta tgtaaagcag ttgaattacc aaaaaagttt
    28621 tggttagctt taatatatgt ttctaatact tccttatcta ctggagttgt tgctgcataa
    28681 tcaaaataaa tcattttatc accttttata ttatacatta aatttactta atttataaaa
    28741 aaataatctg cattaagcag attattcttc attctaacgg ttatagaact ctacaattag
    28801 tgtttcatta atatctgtta agaattcgtc tctttctgga tatcttgcaa atttacctgc
    28861 ttgattgttt tcatcaaatt caatgtaagg ttttcttgat tgcattgctt ctaaagcttc
    28921 tttaatgata tcaagagatc ttgatttttc ttttaatgaa acagtttgac ctggttgaac
    28981 tagatatgat ggaatatcta tttttttgcc atcaactaag atatgtccat ggttaactaa
    29041 ttgacgagct tgacgtctag tactagcaaa gccaattcta taaactacat tgtctaaacg
    29101 agattctaat aactttaaga agttttcacc ttgttggcct tccattttag ctgcataatc
    29161 aaaagtctta cgaaattgct tttcatttaa gccataaaca aaacgtactc tttgtttttc
    29221 ttgtaattgt aatccatatt cagattgctt tttacgtttt tgtccatgtt ggccaggtgc
    29281 atatgttctt ctaaaagaac cactatttct tcttccaaac tcttttcccg tttccaaaat
    29341 agaataacct aatcttctag atttcttcca tactggtcct gtgtaacgtg ccatattcat
    29401 tcctcctttt cttttaaaat agtaaagaat cctaccaaat tacttttaaa aatcgtatct
    29461 aaagtgttca cctaagcagc tagattacta actacctctt aaggatatta ttttaaaaat
    29521 aactaataag tgctgctctt taccgcacct tgtcatttta ctataattta gcttatttgt
    29581 caattaaaac ttattaaaaa ggctctttga aagagccttt tttatcttta ttttttaacg
    29641 cctgcttcta caagcttaac aaaatcatct gcttttaagc tagcaccacc aactaatgca
    29701 ccatcaatat taggcattga taaaatttct ttgatattat caacttttac tgatccgcca
    29761 tattgcacac gcattttatc ggctacttgt ccatataagt cttttacaac ttctcttacg
    29821 taagcacaca tttcttcagc ttgttctgca cttgcagttc ttcctgtgcc aatagcccaa
    29881 attggttcat aagcaataac taaatgttca atatcgtttt tgtttagacc ttcaaggttc
    29941 ttttcaagtt gtgctttaac aacttgtttc tcttggcctt tttctctttg ttctaaagtt
    30001 tcaccaacac atagaattgg aattaatcct ttttcaaaag cttttttaac ttttaaatta
    30061 actgtttcat ctgtttcgtt aaacatagct cttctttcgc tatgaccaac aataacataa
    30121 gcaactccta gtgtagttaa catatcagct gaaatttcac cagtataagc gccgttatct
    30181 gcataatgca tattttgggc accaactctt aaattatctc cttgtctttt gcacaaagtt
    30241 ctaagtaaag ggaacggagc acaaacaaca ctatctactt catcaatatg aggtagtttt
    30301 tctgaaacag cgtaaacgaa ttgtaaagct tcgtcacgcg ttttattcat tttccagtta
    30361 ccagcgatta tttttttgcg cattttttct tttcctttgg tttttcacta tcatttaaac
    30421 aagtaattcc aggtaggtct tttccttcta aatattctag actagctccg ccaccagttg
    30481 aaatatgtgt gaacttgctt tcataaccca tactaattgc agcagcagca ctatcaccac
    30541 caccgataat agtttttgct ccttctaaat tagctaaaac ttcacaaatt cctttagttc
    30601 cagctgcgaa cttttcaaat tcaaatactc cagcaggacc attccaaaca actgtttttg
    30661 ctccatgtaa ttctttttca aatacttcta aagtcttagg tccaatatct aatcccatct
    30721 tatcagctgg tgtatgatga acgtctacta attcagcttc tgcgtcagca ctaaattcac
    30781 tagctactaa atgatctact ggtaaaataa ttttaccttt tgctttcttt aataactctt
    30841 cagcatattt tacataatca tcttcaactt tagattttcc aacttcatag cctaaagctt
    30901 taaagaatgt atatgccatc gcaccacaaa ttaaaatttt gtctgcttta ttaattaaat
    30961 tctcaataac agatatctta tcgcttactt ttgctccacc taaaattgct acaaaaggtc
    31021 tttcaggaac attaactgca tcaccaataa acttaatttc tttttcaagt aagaaaccag
    31081 caactgctcc tcctttgatg tttgcggcaa ctccaacgtt tgaagcatgg gctctatgtg
    31141 ctgtaccaaa agcatcgttt acaaaaacat cacctaagct agcccaatat gcacctaatt
    31201 caggattatt cttagattct ttgtttccat ctagatcttc aaatctagtg ttttcgaaca
    31261 ttaagacttc tccttctttt aatttcttaa tagcggcttc taatttcttg cctcttgttt
    31321 caggaacaaa tactactttt tcacctaata gttctgatag tctattaact acaggagcta
    31381 aacttgaatc ctttttatcc tcttcagttt taactcttcc taaatgtgaa aataatacta
    31441 atttccctcc gttttccttt acatacttaa ttgttgataa agcttgaaca attctgttat
    31501 cattagtaat tactccacct tttaatggaa cgttgaaatc aactctcatt aaaacaactt
    31561 taccttttaa gtctaagtct ctcacagtct ttttactcat aaacaatctc cttctatttt
    31621 ttatatattt tataagttgt acaataccat aataaattcc ataaccaaca aagtatcccg
    31681 ccaaaataat aatgatagct tcaaatggtt tgtctttcat taatgaactt agaaaaccaa
    31741 taacactaaa cataattcct cttgatgcaa ataagataac tccatatctt tttttaccta
    31801 tagaagttaa actcatcgca taatttacga tagctgatgg aatgaaagga acagataaaa
    31861 tgaaaacata aaatcctgta cttttattgc taagccaacg attgaatctc aacacttgag
    31921 gtttggtaat tattgatttt ctaaaccttt tccctattga caagttcaaa actaaaaata
    31981 caagatacat tcccccagca gatccaatca cgcttaaaag aaccccccag aatggcccaa
    32041 gcaaagtaac attggctgtt acgattgcaa gtattggcaa tatcggaaac aacgcttcga
    32101 caagcggcat tcctatacct actaaaatcg caagccaaat agaaccttga gcaaattctt
    32161 gagctgcagc aataaattca ttaaaatacg cagctaatct tttcataaaa ttagctaaaa
    32221 caataatttt atattttcct cccataacat tcatcttctc ttactttttt cttatatatt
    32281 gtatcacaaa atgttatgtt tttgtagagg taatcgtttt tattttttta tttttcatag
    32341 caaataaaaa aagtcaccaa agtgacttct ttaatattat ctattatttt aatatttttt
    32401 aattcttgct tctgtttcag gatcaaagaa gtgacatcta tccatattta atgctaattc
    32461 aacttcatct ccaccataaa gatcgattct agcttcaact ttagcaacta agttttgacc
    32521 agctacaaca ccataaattt gtgtttcagc gcctaataac tcagctacgt caacctttaa
    32581 tttaacaatt gaatgtggat gctttttaag atcttctggt tggtctaaaa tgttttctgg
    32641 tctaatacct aaaacgattg gtttttcgat aaatccttga ctttttaatg tttcaaattt
    32701 atcttttgga acttcaatta aaacgaatcc ttttttgtga tcaccaatta caaattgacc
    32761 tttttcagaa acatatcctt ctaaaaaatt cattggtggt gtaccaataa atccacctac
    32821 gaaaatattg tctggattgt catagatatc ttttggagtt cccacttgtt gaacaagacc
    32881 atctttcata acaacaattc tagatgccat tgtcatcgct tcaatttggt cgtgcgtaac
    32941 gtatactgta gtaactccta aactttcagt aagtttgata atctcagccc tcatttgaac
    33001 tcttagtttt gcgtctaagt tagatagtgg ctcgtccatt aaaaatactt ttgcatctct
    33061 tacaagtgct ctaccaagag caactctttg tctttgacca cctgataaag ctttagggcg
    33121 acggtctaaa taagatgata gtcctagcat gcttgcagca ctttgtactt ttctatcaat
    33181 ttcatcacgg ctaaactttc ttaattttaa tccgaaagcc atgttttcat aaactgtcat
    33241 atgcggatac aaagcatagt tttggaaaac catcgcaata ttacgatcct ttggtgcaac
    33301 gtcattcatg atttcatcat caattctaag ctcgccagaa gtaatatcct ctaatcctgc
    33361 taacattctt agtgttgttg ttttaccaca gcctgaaggg cctactaaaa caataaattc
    33421 tttgtctttt acctctaggt taaagtcaat aactgcgtgc acattaccat cgtacacttt
    33481 atttaagttc ttaaacgata tagtcgccat aaatacctcc caatttctat attttattat
    33541 agcataattt ttcctatatt tcaatatgca gattgcacat tatttatcta aattcaataa
    33601 atacatcgct aaaacatcag taaaatgtct aatgtttaat tgtgtctctt gataaaccaa
    33661 atcgatttta ttatttacag tatttctatg taaatatatt tcattagctg cttgactaat
    33721 gtttaaatca ttattaatca atgcttctac aatcaaaata atttggtggt cgttataaca
    33781 attatttaaa ataattggtc ttaattcttt taatattgct ctactatttt gaaaagtttc
    33841 aaatattaat tctttgtttg aagaaaaagc ataatagtct ttatttaaaa aatcacaata
    33901 agctttgtac aataaataaa agtttttcgg atcatcgcta ctaactttac tacttacaaa
    33961 taccctaaaa ggcaaagaga agtcttccat aacacttaac attaagttct taatatcgtc
    34021 tatttgttgt tctttataaa acaaaacata attgtcttct atttctaaaa taaaagaatc
    34081 atgaaatagt tcattaaaaa atttcaaaat aggtttatga ttgccttttt taaaactaac
    34141 tataataaaa cgataatact tatcatcatc tacaaaaggc ccacctaaaa acaaatactc
    34201 atacattgtt tttattttca tatctgtaaa ctgtaaagat aacaatcttt ttttaatttt
    34261 ttcaattgtc tcttgagacc acatattttt acctcatagt gtattataac aacttttata
    34321 cctttttaca acaattattc ttattttttg ctataatagt ctttgaggtg ataaaaatga
    34381 agttagtatc atggaacgtt aatggtttaa gagcttgtgt taaaaaaggc ttttttgatt
    34441 tttttaaaga aatggatgct gatatttttg ctattcaaga aactaaaatg caagaagacc
    34501 aaaaagagtt tgatgtagaa ggttactacg aatattggtt tgatgctgaa aagaaaggat
    34561 attcgggaac attaatttac actaagcaaa aaccgcttaa tgctactata ggcatagaag
    34621 gaaaatattt agatgaagga agattaataa ctttagaata cgataacttc tattttatta
    34681 atacctacgt tcccaattct caagatgaac ttaaacgttt agattataga atggagtata
    34741 atgatgcttt aaaaacttac cttacaaaac taaaaaaata taaaccagtt attatttgtg
    34801 gggatcttaa tgtagcccac caagaaatag atattagaaa tccagatact aatagaagaa
    34861 atgctggctt tactgacgaa gaaagagaaa agatgacaga actattagat agtggtttta
    34921 ttgacacatt tagatatctt catccaaata aaatccaata tagttggtgg agttatagat
    34981 ttcaagcaag agaaagaaac gtcggttgga gaattgatta tttcttaatt tcagaagatt
    35041 taaaagataa acttcaaaca gccaaaattc acgatgatat ttatggtagc gatcattgcc
    35101 ctgttagtat cacaattaaa ctataggaga aaacaatgaa taatccaaaa gcagatttca
    35161 aaattaaaaa aatagaagaa gaaatcattc ttccatataa tattaaatat caaaaaataa
    35221 aaggtgctac aaaaaccaac tatgaaggaa ctgtagcagg aaacaaaatg ggtgaagcaa
    35281 atatctcaaa tgaaatgata gatgcaaaca aatactactc acaaaatatt gttgccctta
    35341 ctattcccca caatctagat ctaaaattca aacaagtatt taaaaataaa aatagcgttt
    35401 gggaaagaga acatttagat atcttagcgc aagaatatga acaagaaact ggtgaagaag
    35461 taatggctat tattaatgat gacttttttg atatctaccg tatcttccct tttcctggtc
    35521 aaccagatgg tttagtaatc caaaaccaaa acaactataa aacaaaatca tatgggaatc
    35581 atatgagtgg taatttgggg gttgatttaa ataataattt cgtttctttt tctttagaag
    35641 aaagtaataa tttatttgaa aaattaccca aaatagatat cttatcgtct aatagaatta
    35701 aatctttttt aattaactct ataaacgaaa agcctaagca aggagaaatt gctctttatt
    35761 ttggacttcc tggtgatgac caaaaagtta accccaaaga aatagaagga aaaggaaaac
    35821 tatattcagt aaaaggtttg attcttccaa acggtaaaaa tgatttttat gggaaaggaa
    35881 aaattaccaa atccactaac aacattctaa aagaagatat ctttttctta gattctttat
    35941 ccgaagattt caataaatcc attaaagaag gagatttagt tagagtccaa tataatttaa
    36001 aagaagaatt taaagattat tatgctttct ttggttacaa aggaattgtt ttaaaaaatg
    36061 gcgaatttac taaaatggat gggaatttta aaattagaca tccaagaact ataattggaa
    36121 ttaaagaaaa caaagatgta gttttggttg ttgttgatgg tagaagacca gaacaaggat
    36181 cttatggtgt taatggttta gagatggcgg ctattatgaa aagattaggt tgtgttgatg
    36241 cttataattt tgatggtggc ggctctagtg ccttagcaat aaaagaaaaa aataatttta
    36301 tattaaaaaa cagaccttgt gaaggtccaa gaaaagtaat aaatgcttta ataataacaa
    36361 aaaaggtgct tgaataagca ccttttaata tcttaaatca ttacactcgc ttcatacaga
    36421 tcaaaataat atcctttatt tttaagaagt tcttcgtgat tacctctttc cataattcca
    36481 tcttttccta aaacaataat ttcatcggcg tttttaattg ttgaaagacg atgggcaata
    36541 ataatcgttg ttttatttaa agcaagtaaa tctaaagctt tttggatttg tttttcagta
    36601 atattatcta acgaagatgt agcctcatct aataacaata tctttggatt ttttaagaat
    36661 aatctagcaa tagaaactct ttgttgttgc cctccagata atcttactcc tctttctccc
    36721 actatagtat cataaccgtc atctaaactt acaataaaat catgaatttg tgctttttta
    36781 gctgcttctt gaacttcttc aaaattagca tcaggacgcc caaataaaat attttcttta
    36841 attgttccat aaaaaataaa tacatcttgt tgaacatggc ctattgcact acgtaaagat
    36901 ctaatatcat aatcttttac attaattccg tctactaaaa tttctccatc ttgaacatca
    36961 tagaaacgag gaattaattt agacaatgtt gatttaccaa cacccgtttc cccaacaacc
    37021 gcaattttct ttcccgcttc aatagtcaga ttgaaattat ttaataagtg tttcttgtcg
    37081 tcatcttctt cttcgtagtc aaaaacaaca tttttaaatt ctatcttgcc ttcaaactct
    37141 tcttttacaa ctccattttc tggatttaca ataactggct ctttttgcat aacttcataa
    37201 aactgagcaa aaccagacat tccttgttga atttgttcca tagagttaac taatctatta
    37261 attggttgaa ttaaaaagtt aatatacaaa aagaaagctg ttaaattgat aattgtaata
    37321 tgtcctttaa aaacaaataa accaccaaat actaaagcag ctaaattagc catattaatt
    37381 aaaaagttgt ttcctgttcc aaacaaggct aattctttaa ataccttaat tctagctttt
    37441 ttatattgag tattagaatt ttcaaacctt gcttcttcat attctctatt gctgtaagct
    37501 tgtgttaaac gaataccact aatactactt tcaactcgtg cagctagttc tccttgaaca
    37561 actctagttt ctgcaaacct ttcaagcatt ttctttcttc tatataaaga aaaaagaact
    37621 aaaagcaaca taaatagaaa aataattaat gttaattgcc aattgattaa acctaacatt
    37681 ataaacgaac caacaagcat caacccagca ataaaaagat tttcgggtgc atgatgactc
    37741 atttctgaaa tgtcgtgcaa atgcgttgtt aaattagtaa ccaatactcc tgttttgttt
    37801 ttatcataaa attgataatc cattagttgt aacttgtgaa ataagtctct tctcatgtca
    37861 gattcaattc taattcccat aatgtggcca taataaccaa taaaataaga aaagaaaaat
    37921 cttaaaataa aaaacccaat cataatcgct gctattatat ataacatatg gatgttgtct
    37981 gtatttaaat attcattaag aacctttctt gaagctacag gaaaaattaa atcaattgca
    38041 cccattaatg atgctgcaat tagatccgct gtaaacagtt tccaatgtgg tttataataa
    38101 ctaacaaatt ttttaaacat atctatctcc ttacattttt tgattatatc ataattatta
    38161 atttaaaaca aaggaatcac tcctttgttt ttttaaccat tttgtttaat tcatatcttg
    38221 gaataataat tattctcttg catgtcgtgc attctaactt ataatctact ccaactcgta
    38281 atactttcca actatttccc ccacaagggt gattcttttt gaaaactaaa atgtcgtttt
    38341 ctttataaat gtttgcgctc attatttctt atttgtaact acgatttgtg gtagtggaat
    38401 ttcaatctta tgtttatcac aaatctcttt aatcattttg ttaacggctc tttgagctgc
    38461 aaaatgttgt tgcggtagtg cttcaaaagt aatcattaaa tctacaccgt gtgtacctaa
    38521 attagaaact cccataactt taggcgctct tgtaacaaca tctggaaatt gttctactaa
    38581 agtttcaagt tcttgatcaa aaattttgat aactttatct aaatcagagc cataagctac
    38641 agttgcagta actacagcta aagtaggatt acgagaaaag ttaattactt tatcgattgt
    38701 accgttatta aaagtggcaa gttctcctct aaagttttta attttagtcg atctaattcc
    38761 tacctcttca acaaaaccct taaagtctct aatttcaacc acatcgccta catctaaaac
    38821 attatctaaa atcatagata taccggctac taaatctcta atagcatttt gtgcgcctaa
    38881 actgattgct aaaccaacaa ctccagcacc agcaatcatt ggccccaagt ctactcccca
    38941 aattccaaaa actacaaaaa ccaacataac tccaagaatt gcttttgcta aacctctcac
    39001 tagtttatta atagtgattt gtctttttct aaatggatga ttcgccctaa cgtttgcttt
    39061 aaaaataagg ctaaaaatta atgaaattat aatggcaatt acaattgcaa ttgctgtata
    39121 taaaagtcta ttcaaaactt ctgtttgcgt aaaaaaagtt tttatttcat tccaaaattc
    39181 tgaaaacata cttaaaaaca ttattctgtg ttctcctttc tctctaatat ttcttttgct
    39241 aataaacgat attcgcggct tccaattgaa ttataagaaa aagataaaac atcctttcct
    39301 ctagaaggcg cttcttgtaa gtgactagaa tagttaataa cagttttaaa caccttatta
    39361 gccactatct ctttaatatt ttctgccatt tcatatgaaa aaacatttcg attatcaaat
    39421 ttagttaata aaatcccctc tacaactaaa ttctcctgaa tcttagtttt taatccttgg
    39481 atagcgttaa ttttattaat cataatttta attccatcat atgcaaaata ttgacattcc
    39541 gtcggaatca aaactgagtc gcttgcatat aaagccatat caagcaaaac ccccattaca
    39601 ggtccagtat caattaaaac ataatcgtat tcttttttaa taatatctaa gtgtttcttt
    39661 aattcttcaa aattataatg gttatctgat aaagcttgtt tatgaaagca attattagga
    39721 ataagatcaa atcttttaaa actaactata ctatctttaa tatttgtttt tttaagtaaa
    39781 atatcaaaaa ttcctttgtc tatgttttct cgatcaattc ctaaaccagt agttaaatta
    39841 gccttgatat ctaaatccac taataaaact ttttgtttca aatttccaag acctgctcct
    39901 aaattaattg tagttgtagt tttacctaca cctcctttag gattagcaat tgtaataact
    39961 tgtcccataa catttccttt ctataaaggt tttcctttaa ttaaattata tgttcttgga
    40021 tataatgtac ttgtcttttt cttttttaat acctctataa ttactctact tcctaagttg
    40081 tttggaagtt catattttat tatatcacaa atttctgaat ttagggtttt taaagcaaac
    40141 aaagattctt ctacttctat ttgatagttt ttacctttca ttaaaaggac ttttcctcca
    40201 accttaagaa aaggaacagc taactctaga aaaattctca tttgcgatac
//
LOCUS       DAOSGS010000002        39476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_782_length_39476_cov_6.898785, whole genome shotgun sequence.
ACCESSION   DAOSGS010000002 DAOSGS010000000
VERSION     DAOSGS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 39476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39476
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_39476_cov_6.898785"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLQ22_00225"
     CDS             complement(<1..356)
                     /locus_tag="PLQ22_00225"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58418.1"
                     /translation="MKLAGDENVDLYAVDEIISADLLVVSNDLKIERINTLKNILEKK
                     AKIIVTHVQAVLKPLISPKLFKKQIINLKKNKIINREELIKQLVIIGYNRTPITFQTG
                     DFSVRGEVIDIFPILH"
     gene            complement(556..1122)
                     /gene="pth"
                     /locus_tag="PLQ22_00230"
     CDS             complement(556..1122)
                     /gene="pth"
                     /locus_tag="PLQ22_00230"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH58419.1"
                     /translation="MKLIVGLGNPGKQYEKTRHNIGFMFLDEIAKEHNLTFKLQTKFK
                     GATALLKLDQDYILLKPLTFMNLSGEAVKLVMNYYDIALEDILIIHDELALPTGKLKI
                     KPKGSSGGHNGVQNIIDNLDSEEFARIRIGIDNFKDDIIDYVLGKFSRKEMKLLAPII
                     EDAPNMISYLDKQGLEKFMSYYNTKYNE"
     gene            complement(1119..1964)
                     /locus_tag="PLQ22_00235"
     CDS             complement(1119..1964)
                     /locus_tag="PLQ22_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOH58420.1"
                     /translation="MSQILDGQMISKEIKERIKEEVNRLEVKPKLVVILVGDDDASKI
                     YVRNKIKACENVGIATEDYILPKDTREKELVDLIEKLNKNKEVNGILVQLPLPRHLNE
                     DTIVNTISPIKDVDGLNIINQGNLLNRKPVLVPATPKGIITLLKRYFVEIEGKRVVIV
                     GRSKLVGMPLALLFNHANATVTICHSYTEDLKDITKQADILVSAVGKADFISGDMIKK
                     DAVVVDVGINRQMGHIVGDVNFKEAMEVARLVTPVPKGVGPMTIASLLENVLECYDLQ
                     KRDMK"
     gene            complement(2023..2751)
                     /locus_tag="PLQ22_00240"
     CDS             complement(2023..2751)
                     /locus_tag="PLQ22_00240"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOH58421.1"
                     /translation="MDKIERLKEIIESSNNIVFFGGAGVSTASGIPDFRSSSGLYSSQ
                     YKSLRPEYIISHSCFIKDPNLFYTFYKEKMIFPNAKPNYAHIALAKLEKAGKLKAVIT
                     QNIDNLHQMAGSSPSKVIELHGSVYRNKCMKCKEKYDLAYVLDAQPVPYCKKCGGLVK
                     PEVVLYEEPLNNYDIETAINYIARCDTLIVAGTSLTVNPAAGLVQFFRGTNFVIINLS
                     ETPYDFLATLVIKEPVDKVFEKII"
     gene            complement(2837..4258)
                     /locus_tag="PLQ22_00245"
     CDS             complement(2837..4258)
                     /locus_tag="PLQ22_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH58422.1"
                     /translation="MSKKYDCIIVGAGPSGMYCAYELMLKKPEYKVLLIDKGHDIYDR
                     QCPILQGKIEKCPVDRNGYHGCKPSCSISTGFGGAGAYSDGKYNITNEFGGWLNEYLD
                     DDELMELINYVDKLNLEHGANPEITDPYTSEVRKIEKLAIGAGLKLLRSKVRHLGTEE
                     NIQILKNFRDEMANHIDYRFRTKVGDLLIEDDKCVGVVLCDGTEIYGNNVILGVGRDG
                     SSWLEEVLQKHDIKFKNNQVDIGVRVETSNIVMEDINTHLYEGKFIYRSSVGTTVRTF
                     CSNPSGHVVVENQNGAILCNGHSFRDIRYGTENTNFALLVSHEFEKPFNNPNDFAHAV
                     ATLANMLSSGSVIVQTYGDIIRGRRSTKKRLKEGFIRPTLKEAVPGDLGLALPYNTMK
                     SIIEMMDALNHITPGIASDHTLFYGVEAKFYSARPEVNRNFETKIKNLYVAGDGAGLT
                     RGLAQAGANGIHIARSIAKKDKK"
     gene            complement(4264..5844)
                     /locus_tag="PLQ22_00250"
     CDS             complement(4264..5844)
                     /locus_tag="PLQ22_00250"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH58423.1"
                     /translation="MSRAKFIFVTGGVVSSLGKGLNAACLGRLLKDRGLKVFIQKFDP
                     YINVDPTNMSPLQHGEVFVTEDGAEADLDLGHYERFIDINLSKHSTVTSGKVYLSVIE
                     KERRGEFDGQTIQVIPHITNAIKTLIYEAAETSKADIVITEIGGTVGDIESLPFMEAI
                     RQVRRELGYENTAYIHTTLVPYLTAAKELKTKPTQHSVKELRSIGIIPDIIVLRSDRP
                     IPESQKKKIALFCDVKDEKVIDVPDCKSIYEVPYLLKEQKLDTLVLNHFGIKTKPVDL
                     KEWDQMVARINNSKEEVKVALIGKYVSLKDAYLSVYEALSHGGFNNSLKVKIDAIDST
                     KITEKNVAKTLQAYQGIIIPDGFGQEKIEGLMLSIKYARENKIPFLGISLGMQAAIIE
                     YARSNLNLKEANSLELDPDCKMPVITLRKEGKMKLGSSEVEITNGTRIYDVYQKTEVS
                     ERYRHRYQFNKKYLEEFKNGDLVWSGFSKKGTPVAMELKGHPFFIAVQYRPEFKSRPN
                     RAHPLFGEFIKACYENKR"
     gene            complement(5964..6503)
                     /locus_tag="PLQ22_00255"
     CDS             complement(5964..6503)
                     /locus_tag="PLQ22_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58424.1"
                     /translation="MEKDKNIYEDMSMVDVALKILEETKKPDTIVNIAAEVFKRKGIE
                     KVDPQDYIQFEIDFMLSGNFICCAEDLEGTRLWDLKYRQNSKLLNQDKTYIDVVDEVF
                     SEDALKYEMQEELLAQYEDEVLEEDDEEGDDEVIYDDISDEIDEDDEEEDDEDYDEYE
                     DLDDEDYDEDEDDEFEDLI"
     gene            complement(6490..7221)
                     /gene="surE"
                     /locus_tag="PLQ22_00260"
     CDS             complement(6490..7221)
                     /gene="surE"
                     /locus_tag="PLQ22_00260"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH58425.1"
                     /translation="MKILVVNDDGIQSEGLKRLVERTKKYGEVFVVAPKYNQSATSHK
                     ILVRGGFSVEKINLFPGVEAYAIDSTPADCVRFAHYHLKCDFDLVLSGVNKGYNLGFD
                     IFYSGTVAGATEAAYTKRRGFALSANYKSLAGFEKYFDEVMELLLSEEYWNKALVYNV
                     NFPIEPKGIKLAVQGDTCFDTYFEEKEGLYYQLGTGNLALDNNENSDAYLTNRGYVTI
                     TPLSGDRTNQEVYNLLKGGVKDGKR"
     gene            complement(7223..8200)
                     /locus_tag="PLQ22_00265"
     CDS             complement(7223..8200)
                     /locus_tag="PLQ22_00265"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOH58426.1"
                     /translation="MKKTRNQILKKIKEYDSIIILGHQRPDGDCVGSTLGLKNILTTS
                     FPKKKIYAPANDSASYLDFLKPRDEELKPEIYQESLVIVLDTATKERIDSKYFHLAKE
                     TIKIDHHLPVDDYADINFVDVTAPATAQIIVDLFLYPPKNLEMEQEGAEALFTGIVTD
                     TGRFKYSNVRSQTLNAASLLLDYDIDFEKIYNALYLKDSEVLKLEGYLYLNFKTTENG
                     VAYFYIDKKIRKQYNITIEDAASLVNCLDSIKGSLIWVLFVEQDDSTIRARIRSRYVS
                     IDDIANNYRGGGHKQASGATLLAEKEIDSILEELDIRLKEFKEENGGLM"
     gene            complement(8197..9144)
                     /locus_tag="PLQ22_00270"
     CDS             complement(8197..9144)
                     /locus_tag="PLQ22_00270"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOH58427.1"
                     /translation="MNFNQIKEKVIQYDLIIIERHANPDGDAMGSQLGLKAILKENYP
                     KKKIYAVGDTSERFDFMGKTDEIDIELYKKALVFVLDTSTDEMLLNDNYKKAKFSIKI
                     DHHNTVSDYCVLNYTDTSFESCAGIIAQIALDLDWKLNKEAATHLFTGIVTDSGRFRY
                     DKTTARTFDIVSKLMEYDIDIDNVYSNLYQESLDSVKLRAKLTNKFKVSKHKVAYLKV
                     TKKDVEAYGLMPHNIARGYVNIMANIKEIEVWAMFVEDAENNQILCEFRSSKHNINQI
                     AVKYGGGGHLLASGAKVASFEVCREIIKDLNKLVAGSKK"
     gene            complement(9134..9715)
                     /locus_tag="PLQ22_00275"
     CDS             complement(9134..9715)
                     /locus_tag="PLQ22_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOH58428.1"
                     /translation="MLHNRVNKVQELAIASIMVAMAAVLSFFDKSISVIAFPFLPVAK
                     IGLANIVILLAIYIFDFKTSLLITVLKSVVVGLILGSISSFIIGASASLVSFFWMFLA
                     HKLLKDKTSLVGISVIGGFLHIVTQLFVVMLIYHTGDAVFKSGALLVLISLVTSIIVG
                     IAGNGLYKYLTIHYHLNSSLELVSEDKGENNEL"
     gene            9806..10204
                     /locus_tag="PLQ22_00280"
     CDS             9806..10204
                     /locus_tag="PLQ22_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOH58429.1"
                     /translation="MCIFCKIANKEIPSYIIYEDDKFLAFLDLSQITIGHTLVVPKKH
                     FANLLELDEETAKEALNVTRKVALLLKEKLHVFDFNIVNNCGEKAGQSVHHFHIHIIP
                     IYKKGDLEFNTQSNKLSEAEFKKLHNKIIK"
     gene            complement(10225..10602)
                     /locus_tag="PLQ22_00285"
     CDS             complement(10225..10602)
                     /locus_tag="PLQ22_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH58430.1"
                     /translation="MYCRKCGTQVQEDAIFCHVCGNLLEEAEQVNVAEEQAYTTVEKR
                     KREKNSFDTINFILTIISVSLMVVAFIPYVPIVALILGGVALIIAIILEIKKKYKFSL
                     LSLYLAAGGILANTLLLIYFSNI"
     gene            complement(10607..11881)
                     /gene="serS"
                     /locus_tag="PLQ22_00290"
     CDS             complement(10607..11881)
                     /gene="serS"
                     /locus_tag="PLQ22_00290"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH58431.1"
                     /translation="MLDIKYLRENLEEVIKNLNQRGSDFSYLRDVIKLDDEKRNLITE
                     VEKLKKMRNDASKEIGILKREKKDASSVLAEVASLGDEIKAKDEAIKELDEKIFNYLA
                     TTPNLVHKSVPIGPDDSYNKEIKKFMEPTKFNFKPKAHYELGEALDILDFERAAKVTG
                     SRFAIYKGLGARLELSLIKFMMDLHAKSGYTEVIPPYIVSAASMFGTGQLPKFAEDAF
                     KIANGNGDWYLNPTAEVPTINMFKEEVLDYAKLPIKLTSYTTAFRSEAGSAGRDTRGL
                     IRQHQFNKVELIKIASPDNSYQELDEMLAESEKVLKALKLPYRVVVLSSGDMGFAMCK
                     TYDVEVWIPSQNTYREIGSISNAEDYQARRANIKFRRSQDSKLEFVHTLNGSGLAVGR
                     TLVAILENYQNEDGTIKVPEVLKPYMRVDKIG"
     gene            complement(12057..14495)
                     /gene="gyrA"
                     /locus_tag="PLQ22_00295"
     CDS             complement(12057..14495)
                     /gene="gyrA"
                     /locus_tag="PLQ22_00295"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH58432.1"
                     /translation="MKNSFLSYAMSVIVSRAIPDVRDGLKPVQRKILYAMHGLGLYSD
                     KPYKKSARIVGDVMGKYHPHGDSAIYDAMVRMAQDFSFRYPLVEGQGNYGSIGGAQAA
                     AMRYTEARMSKISMELLRDLNKNTVDYQDNYDGTETEPVILPSRFPNLLVNGGTGIAV
                     GMATNIPPHNLEEIMDALLAVVKNPKITVEELMEYVKGPDFPTGGILMGLTAIREMYK
                     TGQGSLVVRAKTHYEDLGRDRQQIVVTEIPYAVNKTLLVDRIVKLVKDKIVEDITDLR
                     DESTRDGLRIVIELKRGANSGVVLNNLFKHTQLQSTFSTNMLVLDGGQPKTLNLKEIL
                     ECYLAHQNEVIRRRTEFDLEKAEARAHILEGLIIALANIEEVVKVIKASKSGEIAINR
                     LMEKFVLTEEQSKAILDMRLQRLTSLEVEKIKEELVELEKFIAECKEILQNPQKKKEL
                     IIKEITETKEKYKDPRRTEISLAEDLGLEDENLIPVEDVIITITERGYVKRMTSDTYQ
                     TQNKGGKGIIGTKMVESDAVTKVIYTSTHDDLFFFTNFGKVYTLKAYQIAEANRLARG
                     TPIVNLLKLENNERLATVLSIDTDAIKETDYLFFATKQGLVKKTQVEEYKNIRRNGII
                     AINLVEGDELLSVGITDGNKNIILGASNGKAIKFSEDDVRPMQRVAQGVRGILVEEGE
                     EVVGMAIINSPNDEIVIVTENGYGKRTSVREFRTQVRGGKGVTALSINKRNGQMKAMK
                     TVESEEDLIVITNYGFIIRVPLEQILTIGRITQGAKIITLNANQKVASIEILPKEESE
                     ADIDSDLDLFVEYE"
     gene            complement(14614..16530)
                     /gene="gyrB"
                     /locus_tag="PLQ22_00300"
     CDS             complement(14614..16530)
                     /gene="gyrB"
                     /locus_tag="PLQ22_00300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOH58433.1"
                     /translation="MSDDKRLNSQYSGSDIQILEGLEAVRTRPGMYIGSTSDTGLHHL
                     VREIVDNSIDEALAGYCDHIEVEIQPNGSISVTDNGRGIPVDIHPKTGESALEAVFTV
                     LHAGGKFGGKSYKVSGGLHGVGASVVNALSEWLKVTVFKDGYVYEQEYGRGKKLYDVR
                     RIGITDKTGTVVLFKPDATIFKEGTEFDYETLASHLQQSAFLNKGLKLTLTDLRNPYR
                     VRTNTYQYDNGIIEYVKYLNQSKTPIHNEIIYLSGKVDNVEVDIAMQYTDDETCRIHS
                     YANNINTPDGGTHEEGFKTRLTQIINQYGKKIGLFKKKESLTGDDAREGLTAIVSVRH
                     PSPEFEGQTKSKLGNADARRIVSQILGDQLEQYLDENPETGKEIINKALESSRARLAA
                     KKAREASRKSPLETIGFASKLSDCRSKDPTISELYLVEGNSAGGSAEMGRDAEFQAIL
                     PLRGKVLNVEKATKASVVSNNEIISMIQAIGAGIGKEFDIESVRYHKVVIMTDADVDG
                     AHIRTLLLTFFYRYMRPLIEAGYVYIAQPPLYKVSYGKRIEYAYSEAELQDVLETFNA
                     KPNIQRYKGLGEMNADQLWETTMDPKTRTLLKVKLDNVAVADTVFSMLMGDDVAPRRE
                     FIESNAIYVENLDV"
     gene            complement(16535..17608)
                     /gene="recF"
                     /locus_tag="PLQ22_00305"
     CDS             complement(16535..17608)
                     /gene="recF"
                     /locus_tag="PLQ22_00305"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOH58434.1"
                     /translation="MFYIQKLKLINFRCYKDKKIIFKPGVNIIVGKNAVGKTSLAEAI
                     YCLSLTKSYKASRDSLLIQHDQPYYVLEGEFINKAISHNVLLSYELDKLRKRLLVDNN
                     VIKKMSDYIGTFRVVMFSPDDSELIKGYSPQRRRFLDVYLGQINNKYLKDLSNYNRVL
                     KNRNELLKKPNPSEQDLEVLKIYSEALVKYGINLIIERKKFIEMLNPEVAKISLAISN
                     ETEKTELKYMPNVEKDDFLLKTFRQLKKDLYLQTTGLGPHKDDFIFEINGKDAASFAS
                     QGQQRTSSIALKLGLANLFDKLGMDNIVILDDVFSELDKERQNQIMQLFKEDKQIFIT
                     TTTIDSIHETLLENSNIIKIERE"
     gene            19178..19528
                     /locus_tag="PLQ22_00310"
     CDS             19178..19528
                     /locus_tag="PLQ22_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58435.1"
                     /translation="MMDKLKKRASSPLSGLDKTVNTATTVSEESQPEPIVQEPVAYKP
                     EPAPATPKQTTQKTAKKGGFEVFGGDEKIKYTATMSHEVRANAKIAAISLGWTFSEFV
                     EEAVRAYLLTRRIK"
     gene            19538..20422
                     /locus_tag="PLQ22_00315"
     CDS             19538..20422
                     /locus_tag="PLQ22_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOH58436.1"
                     /translation="MAIISTYCKKGGVGKTTFIGYLAHYLTEVKNKTVLIISVDDQNS
                     IFTLFGKQNLVTASNDDYLEHLMVGLRTYEEVLHEVRDNLYLIKTLNTDALSTKLIID
                     RSSEKTLLEIIDTYNEAFDYVLLDFPPSSCRATEVLLDYSDRIMLIISLDSLGIDGFI
                     NTIQFFVDQDIPISKIQYILPNKFIANRRAPKKSLETLQAQAEEFSKTMELDALILPY
                     FSEKSTVQNIQEQGKTPFDKDELFTYRYDFNEMLKVREELLGVFKAVDEYEEELANRA
                     KKGKEKEKPGFQLKKKKK"
     gene            complement(20466..21593)
                     /gene="dnaN"
                     /locus_tag="PLQ22_00320"
     CDS             complement(20466..21593)
                     /gene="dnaN"
                     /locus_tag="PLQ22_00320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH58437.1"
                     /translation="MNFTINRDLLLENLTNVSRALSTKTPMPVLTGIKFEITKSKMFL
                     TASNNEISIQAQINEGNKLKLEGEGSFVVPGKILIEIIRKLDANEVQFKAVEDNVIKI
                     YANRSDFTLNALEKDNFPLISFAESKSFIKLDIGTLKQIIRKTTFATSLSESRAILTG
                     VCLNLSNSKLEAISTDSFRLAKIYLNVNEELPEVSVVIPSKSLDELNKVIIDDNESDI
                     ELHFSPTKILFKYKNLLFQSRLIDGVYPNTSSLIPTEFLTSIKFNKAELVKTIDLMSL
                     FTNIESSNIIQLQLNPDKTVEIKSTTSEIGEGKEELIPLECSNPVSFKIAFSAKYFLE
                     AVRSFDSSEIVVHFTGEIKPFIVTGEYDVNLTQLILPVRVS"
     gene            complement(21662..>21946)
                     /locus_tag="PLQ22_00325"
     CDS             complement(21662..>21946)
                     /locus_tag="PLQ22_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH58438.1"
                     /translation="RDIKEAVASYYQVSIPELESGSRKAKIVFARQMAVYLIRTLYDL
                     PLKQIGSYFGNRDHATISYSIDKIEKDMKQNKGIKQDALYLTHKIQGEKQ"
     assembly_gap    21947..22046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22047..23211)
                     /locus_tag="PLQ22_00330"
     CDS             complement(<22047..23211)
                     /locus_tag="PLQ22_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOH58439.1"
                     /translation="MNNLNVENALDIWQKTIEHLKNQIDDISYNDLFSNTKEVFKVKD
                     STIWIIVDSIFTKFRIDKFYKNGINEFALNNFGCHFAFIDRKEAEEQRNKVDLVNTTQ
                     TDIDNSKKPRSLLPEYSFYNFVIGESNRFAFLAATKVANDPIPVLNPLYIFGGVGLGK
                     THLMFAVGNEILQIKPDTNVVYTTAQQYAQDYFTASSSKDPKAFQKFYDYYQSADVLL
                     VDDIQFLEQKTKTQEEFFKLFELLYTEKKKMVVTSDRPANQLQNFMDRLKSRLSYGLA
                     VDIKKPDKQLRLGILQKKLPTHIPNPEEVPLEVLELIAEIYEDRNVRELEGALSRYIW
                     YCVSGNLPFTKENVYPILKPLVEGLDKEGAPADVKVRDIKEAVASYYQVSIPEL"
     gene            23415..24641
                     /locus_tag="PLQ22_00335"
     CDS             23415..24641
                     /locus_tag="PLQ22_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH58440.1"
                     /translation="MTKRGNNVKEVKKITKKISSLSLKVIISAVLVLLISLLLVGCFS
                     NPHIVKDVKVRIDKYNLNKNSSQTIIYEIVPETAAPYNKVTFTSSNTEVLVIDQDGKM
                     TALKEGETIVTITAGKISKTYKITVFFLEIDDEMEEPESDGFYMYVEEAEGTYYASAN
                     GMEGADLKTELNRILRDGFTPRSYGDAREILGRADADLEDPTKVWTIYSGAKINATWD
                     GKSWTREHVWPFSRLGTGTSNIGNSTRNQASDLHNLRAIVQRVNSSRGNAYFTNPITE
                     TTTYCAVTSNSWFPGDDHKGDVARILLYMDVMYEELSLVETGAELLTGETYQLSGAKM
                     GLLPILLEWHRQDPVDDFEIQRNDIIYEEQNNRNPFIDKPEYVHLIWEGKTINDLVKL
                     WFKPIYYYKEEYTYVF"
     gene            24631..25104
                     /locus_tag="PLQ22_00340"
     CDS             24631..25104
                     /locus_tag="PLQ22_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58441.1"
                     /translation="MCFRKKKEIILESGYYYIGDLYYTITGKPASKKLEEKLLKKFYK
                     KNKQLAKLKKIKCQGIKLYAFSPNNNLLLYSKKEVINEIVLETKVICILNLTKYGKHK
                     LFPFSSLSTENFPNSGFIYLNEDTTIAETEKGDIIIGNYSIKEKTKRRTRRKKKE"
     gene            25101..26009
                     /locus_tag="PLQ22_00345"
     CDS             25101..26009
                     /locus_tag="PLQ22_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58442.1"
                     /translation="MKLIKFPEKLNNFTILAILMLYAVAAVVLGYFAIQNKEIVTATS
                     YAEHVENEKGYFNFIKISNRLAKEENVEGNPLTNRYYITGYVRGTSAIKVDLFKASYS
                     VSYVDGKAYYFSTLDSFSHTNSYVMASNSKGADLEAFTSIIHFYDDVEDENGGTTKEE
                     YVYKFTEPMLTFDEKEKITDLEAVKDIFLLNFIPKKKTGKTDYYFNLDFRSKVANYEF
                     DVQGFVKTDKGEIFTTVGLYHFEGNYYGEDIINAHVLENEYITEVTHPKTLYFKVKYY
                     DKISKETNYYLFEIPFPADFYQEESE"
     gene            26093..26230
                     /gene="rpmH"
                     /locus_tag="PLQ22_00350"
     CDS             26093..26230
                     /gene="rpmH"
                     /locus_tag="PLQ22_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOH58443.1"
                     /translation="MSKRTYQPNKRKRSKVHGFRARMSTVGGQRVLANRRRKGRKKLS
                     A"
     gene            26299..26670
                     /gene="rnpA"
                     /locus_tag="PLQ22_00355"
     CDS             26299..26670
                     /gene="rnpA"
                     /locus_tag="PLQ22_00355"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH58444.1"
                     /translation="MNRKYSLKKNHDIKELLQKKQSVGNKYYVIYYDYIENKTPLIAL
                     SVSRKVGNAVERNYEKRVVREIIRPLLETIENYKMLIVIKDKSKELSYVEKEENINYL
                     IKKMKTQKETINEKNLKQQNS"
     gene            26639..27589
                     /locus_tag="PLQ22_00360"
     CDS             26639..27589
                     /locus_tag="PLQ22_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOH58445.1"
                     /translation="MKKILNNKTVKMIGAIVLVLFVALVLTGCGCSGQPTEGGVAAPD
                     PIVGFKSFWDLFVWPMAAIMWVVGKVMGGNYGLTIIFTTILVRTAAWPIYTKTNDMSL
                     KTKLMAPDMEKLEAKYAGKDDKESQQRKQMEMMQLYKKYGIGIGGCLLPFLQMPLFLG
                     FFQALRRIPDTLGAEYPLDFTFLKSNFLGLNLFASRTTAPEMATKIWILAIAVGVLQV
                     LSQVLIIIRQKLQEKKVYSDVPEYRRPQQNQQNKSQNMMMNVFAIAMSVMMVVFVLNN
                     PAGLGLYWLVGNIYTMIQAQISYMLTEKRLAKLKEKFNKE"
     gene            27593..28204
                     /locus_tag="PLQ22_00365"
     CDS             27593..28204
                     /locus_tag="PLQ22_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOH58446.1"
                     /translation="MEKKTYEVKSIEEAYELVKEELGLDKDQVNIEVVEKKGLFKTKM
                     VVEVSEKQDPVKIGEEYLKEILKANNIKGIVEKKVRDNLIYYNINAGDDNGYLIGRRS
                     RYLIALQTLVSTAIYNVTKSMDINVIVDVAEYKQKRIDYLEGLAAQHARRVKETKKPI
                     ALSNLNAYERKIVHDKVASLKGVTTHSEGEGKDRHLIIEPTRD"
     gene            complement(28241..28933)
                     /locus_tag="PLQ22_00370"
     CDS             complement(28241..28933)
                     /locus_tag="PLQ22_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH58447.1"
                     /translation="MEKFLQEKYEAFKKLAAERFSYDKIDLEFITDLVYNSLTFERRA
                     VTREDIKNILSGNHAKYDEKLVTTAENHRKAFLFMVDLAKKGESLTESKLKDCHSILM
                     DEHGGLYRTFNISIQGSQHTPPPHYKIYSRMDEYFEVLKSADFPINKIAYSHLQLSKI
                     HPFLDGNGRLARLVLNYYMLASGLAPIIIPSADKENYFQCLEDYKVKKTTLTFINYLI
                     QLEEASFEKYFK"
     gene            29057..30409
                     /gene="mnmE"
                     /locus_tag="PLQ22_00375"
     CDS             29057..30409
                     /gene="mnmE"
                     /locus_tag="PLQ22_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOH58448.1"
                     /translation="MYDDVICAISTANTRSAISIVRLSGKGSIDIVNKIFRGKDLTKV
                     ASHTIHYGYIYDGDQMLDEVLVSVFKAPRTYTKEDVVEINCHGGVFVTNKVLELCIVK
                     GARLAEPGEFTKRAFLNGRIDLTQAEAVMDVIDAKNKETLKMSNYGLRGDIKRIIQEF
                     RSKILEIILKIEVNIDYPEYEDEEQITKEYLIPSIKENIEEIEIIIAKSENSIKIKEG
                     ITTAIIGKPNVGKSSILNALLRENKAIVTDIAGTTRDVVEGSVNIGGLTLNLIDTAGV
                     RQTDDIIEQIGVLKTQEVLEKAELIILVFDYSSKLDSNDLKIINLTKDKHVLTVINKK
                     DLPQKIDLSLFDNYIIISAFDEKDIETLEKAVKKVCDIQQIDDSEVKYVTSIRAITKL
                     KKALQSLKEGLKALESGMPIDLASTDLRSAWQNLGEILGEVSSEELLDEMFSQFCLGK
                     "
     gene            30431..31030
                     /locus_tag="PLQ22_00380"
     CDS             30431..31030
                     /locus_tag="PLQ22_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58449.1"
                     /translation="MKTEAVLKKNLLSIIGGGILVSGLIVAVVSAIFGGGIPGILSLV
                     CVLGAIFGLALAIATEKFLYFIISFVSLALFAHTYMDVNNVIYLFFFLSLLVAVAGIV
                     YYGIKVKGKILTAIAVFALAAVSLALFIFALVREAPAAFVFVAVGLLLSFSGQGIIVL
                     AGDSKGEFFAEKVANLKQKENEAKAKEKEEKEKEVINLD"
     gene            complement(31082..31993)
                     /locus_tag="PLQ22_00385"
     CDS             complement(31082..31993)
                     /locus_tag="PLQ22_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH58450.1"
                     /translation="MRNFELNISRFFRSISTETLDKIVQILTFMGSQMVIIVLVAIIY
                     FVLDKKAGEKIIYIALTSYGVNNALKGICAVKRPFQVDPDIEAAGVEDATGYSFPSGH
                     SQGAASVYFSIANHFKKTWLWIVAIAIIVIVGFTRIWLGVHFLSDVVVGISLGIGVSL
                     LCGLLYDTFQHSFKSKIILFATTLVIFAPFLYFFYRPNANDMLVYRDFYISYAAYLGF
                     FGAVIIENKLVKFTDLTTLGMKILRAAIGIVIMLVVYLGLKFVFPDGYLILDMVRYFL
                     VIFIGLGIYPLIFKNTRLFKYIYKEVE"
     gene            32109..33068
                     /locus_tag="PLQ22_00390"
     CDS             32109..33068
                     /locus_tag="PLQ22_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58451.1"
                     /translation="MSRKKLSLKTKKIIINTIIIILTIAIPLAITAFVADYGFNKRYN
                     PNSEYADKTITDQIASDLRSDAFIVLMEGATFPRERIQKYVGYRSQKYNSTPIYEETI
                     YNDNNEKLFNMIVYDGLWKRIEEGVEYYDIVSPQIYIYDVQYGKIKEEFEVDPTVIED
                     IEDIELSVKFTIPYKRDSGVADEERQYSFADVNGAITDTGSDLNTKQVQACEFRAALR
                     FAETENITVNFNAKITITSSNIVDQVFKSINLPINSDASTRNLDNFEEGLNSSFDSII
                     APQLKAVVFKKYIWWQCLIALVVSSVVIVPFGLSFILPLKEEK"
     assembly_gap    33171..33270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33271..33711
                     /locus_tag="PLQ22_00395"
     CDS             <33271..33711
                     /locus_tag="PLQ22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH58452.1"
                     /translation="AQDLGCNKIALGHHMNDVIETTMLNILYAGCFKTMMPKLKSKNF
                     EGMEVIRPMYYIKEKDIITYMKYSEIETMQCGCRIASRDLDSKREEVKQLIKKLKKTN
                     PVIEKHIFKTALNVNLDCVIEWEYNNKKYNFMDFYSESKKSDDD"
     gene            33815..36439
                     /gene="ppdK"
                     /locus_tag="PLQ22_00400"
     CDS             33815..36439
                     /gene="ppdK"
                     /locus_tag="PLQ22_00400"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH58453.1"
                     /translation="MSKQRYTYMFNEVDMTMRNLCGGKGANLGQMTKLGLPIPEGFIV
                     TTETCSYFYENGKKLSKEIIEQIKESLKILEKQTGKKFGDPNNPLLLSVRSGARVSMP
                     GMMDTILNLGLNDIVAAGLARKTNNPRFVYDSYRRFVQMYADVVMGVGKSEFEKIIDE
                     MKEERGIKLDTEFSADDFKKMIQKFKTIYKKDVGKDFPENPEEQLFGAINAVFLSWNT
                     PRAIYYRRMNDIPHEWGTAVNVQSMVFGNMGDTSGTGVAFSRNPATGEDHCYGEYLMN
                     AQGEDVVAGIRTPEDLDHLKDYSEELYDELIGSMKKLEAFYKDMQDIEFTIEDNKLYL
                     LQTRSGKRTAQAAIKIAIDMHNEGLISKEEALLLLQPAQLDDLLHPHFEVEALKAAKP
                     IGQGLPASPGAATGEVAFTVEKALEAHKAGRKVVLVRLETSPEDIEGMDISEGFLTAR
                     GGMTSHAAVVARGMGKCCVAGCHELRFQKDYALVNDVKIKEGDIISLDGTTGRIYKEA
                     IATEPATISGDFATVMKWANEAKRLGVRANADTPRDAKQAREFGATGIGLCRTEHMFF
                     GEERIKAIREMIIADCTKEREKALAKILPMQEQDFEEIFEAMGDYSVTIRLLDPPLHE
                     FLPKEEKDIVALAKDLGVSLDRMKLRISELEEANPMLGHRGCRLAISYPELPIMQTKA
                     IIGAYCAVKKRNPAYKAVPEIMIPLIGEIRELRYLKDVIIQTAEEVLKANKMKLEYKV
                     GTMIEIPRAALLADIIGAEADFFSFGTNDLTQMTFGFSRDDGADFLKTYYEKKIYLND
                     PFARLDQEGVGKLIEMTVQKGRKANKKLKIGICGEHGGDPSSVDFFHRAGLDYVSCSP
                     YRVPIAILAAAQAAIKHK"
     gene            36520..37257
                     /locus_tag="PLQ22_00405"
     CDS             36520..37257
                     /locus_tag="PLQ22_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58454.1"
                     /translation="MKYYKYHEEGNDFLIGKEKLNLDYKKEAIKLCNRKLGVGAKGLI
                     VYSNNNINVFNSKGEKKDIYFGGIRAISTCFNDQGIDYKNEINYSGNKIKIIDEDLIG
                     YELKIDQSKLAEDEILTTKGKIPIYRYLDKKEQVLLITKNLDELVNSGLAEELYDNPY
                     FSKEKDISFARIINKDNVVIYTYNEDGWLEGKDLSCAFVYSILEYKEMISKEIRVHLK
                     KGILNLKKENDKLYIFGKANKICKIEI"
     gene            complement(37315..38697)
                     /gene="asnS"
                     /locus_tag="PLQ22_00410"
     CDS             complement(37315..38697)
                     /gene="asnS"
                     /locus_tag="PLQ22_00410"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH58455.1"
                     /translation="MDLYLLDQKHKQLENKEITVQGWVRSNRDQKEFGFIALSDGTTL
                     GTLQLVYDDKLENFGEVSKIRVGASIAATGLVVLTPNAKQPYELKVTKLELIGDSPED
                     YPIQPKRHSREFLREQAYLRPRTNLFNAVFRVRSVLAYAIHNYFYENRFVYLHSPIIT
                     ASDAEGAGDMFQVTTLDLENLPKTKDKKVDYSQDFFRKAAHLTVSGQLEAETFATAFK
                     KVYTFGPTFRAENSNTQRHASEFWMIEPEICFADLNDNMALIEDLLKYVIKYVLKHAP
                     EEMAFFDQFVAKGKIKELETFLNSKIAICTHKNAIKIMKESGHKFTEKPEYGKDLATE
                     HEKYLTDVHFKGPVFVIDWPKDIKAFYMRLNEDGETVAAVDLLVPGCGELVGGSQREE
                     RYDLLEKRMKEMNVPVEDMQWYLNLRKYGTAKHAGFGVGFERLVMYVTGIENIRDVLP
                     FPRTPGNCEY"
     misc_binding    complement(38741..38950)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(39029..>39476)
                     /locus_tag="PLQ22_00415"
     CDS             complement(39029..>39476)
                     /locus_tag="PLQ22_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH58456.1"
                     /translation="ITTTEILNFLAISESTPGPFAINTATLIGFQQAGILGGVVATLG
                     VVTPSFLIIYIIAKFMSKFSENYYVKSVFKILKPVIIGLLMAVVVSLVSKNLFIGEET
                     GYLLDWKAIVIMVVILTLSQIFKKAHPVLLIFISGVLGYIFYGLIP"
BASE COUNT        13445 a         6232 c         5724 g        13875 t
ORIGIN      
        1 tcatgcaaaa tagggaagat gtctattacc tccccacgca cgctaaaatc ccccgtttga
       61 aaagtaatgg gggttctgtt ataacctatt atcactaatt gtttaattaa ctcttcgcga
      121 ttaataattt tgtttttctt taagttaata atttgctttt taaatagctt gggactaatt
      181 aaaggtttta atactgcttg aacatgagtt actataattt ttgctttctt ctctaaaata
      241 ttttttaaag tattaatccg ctctattttc aaatcattgc taactactaa taaatcagct
      301 gagataattt catctactgc atataaatct acattttcat ctccagcaag tttcaaaaaa
      361 gcattatatg cttgattggc tttgtagaga tttgaagcaa tatagataat tgattctcct
      421 tttaaaacat aagcatcata tgcagctaaa tacttagcat tgtttacaac attatataaa
      481 taaatcgatg aattatcttt gatgctttct tcatactcaa taacactatt attaattcta
      541 aataattcta aaatattatt cgttatattt agtattatag taactcataa acttttctag
      601 accttgtttg tctaaataag aaatcatgtt tggcgcatcc tctataatag gagcaagcaa
      661 tttcatttct ttacgagaaa acttgcctaa cacataatca ataatatcat ctttaaaatt
      721 atcaatacca attcttattc tcgcaaattc ttcagaatct agattatcaa taatgttttg
      781 aacaccatta tgaccaccac tgcttccttt aggttttatc tttaactttc ctgttggtaa
      841 agcaagctca tcatggatga ttaagatatc ttctaaagca atatcgtaat aattcattac
      901 taatttaacc gcttctcccg ataagttcat aaatgttagt ggttttaaca atatataatc
      961 ttgatctaat tttaataaag ccgttgctcc tttaaacttt gtttgaagtt taaaagtaag
     1021 attatgttcc tttgcgattt cgtctaaaaa cataaagccg atattgtgtc gtgttttttc
     1081 gtattgttta ccgggattac ctagacctac tattaatttc atttcatatc acgcttttgt
     1141 aagtcataac actctaagac attttccaat aaactagcaa tcgtcattgg tccaactcct
     1201 ttaggaactg gtgttactag tctagcgact tccattgctt ctttaaagtt tacatcacca
     1261 acaatatgac ccatttgtct attaatacct acatcaacta caactgcatc ttttttaatc
     1321 atgtctcctg aaataaaatc agccttacct acagcagata ctaaaatatc tgcttgtttt
     1381 gtaatgtctt ttaagtcttc agtatatgaa tgacaaatag taactgtagc attagcatga
     1441 ttaaacaata aagcaagtgg catacctaca agtttgctcc ttcccacaat aactactctt
     1501 tttccttcta tctctacaaa atatctttta agtaaagtaa taataccttt tggtgttgca
     1561 ggcactaaaa caggtttacg atttaaaagg tttccttggt tgattatatt taacccatca
     1621 acatctttaa ttggagaaat agtattaaca atagtatctt cgttaagatg tctgggaagt
     1681 ggtaattgaa ctagaattcc atttacctct ttgtttttat ttaatttctc aattaaatct
     1741 actagttcct tttctcttgt gtcttttggt aaaatataat cttctgttgc tatccctaca
     1801 ttctcgcaag cttttatttt attacgaaca tagattttac tagcatcatc atctcctacc
     1861 aaaataacaa ccaatttggg tttgacttct aatctattta cttcttcttt gattctctct
     1921 ttgatttctt ttgaaatcat ttgaccatct agtatttgac tcatatctta ctcctttttt
     1981 ctaattatat cattttttaa taaataagtc tttatatttg ttttaaatta tcttttcaaa
     2041 aactttatct acaggttctt taattactaa agttgcaaga aaatcatatg gtgtttctga
     2101 taagtttata ataacaaaat tagtccctct aaaaaattga accaaacctg cagctgggtt
     2161 aactgtgaga cttgttccgg ctacaatcaa ggtgtcacaa cgagcaatat agttaattgc
     2221 tgtttcaata tcataattat ttaagggttc ttcgtataat acaacttctg gtttaactaa
     2281 accgccacac ttcttacaat aagggaccgg ttgtgcatct aaaacataag ctaaatcata
     2341 cttttctttg cacttcatac acttattacg atagacactt ccatgtaatt ctataacttt
     2401 actcgggctg ctacccgcca tttggtgtaa attgtctatg ttttgtgtta taaccgcttt
     2461 tagttttccc gccttttcta atttagctaa agctatatgg gcataattag gttttgcgtt
     2521 aggaaagatc attttctctt tatagaaagt ataaaaaagg ttgggatctt ttataaagca
     2581 agagtgagaa ataatgtact cgggtcttaa gcttttatat tgacttgaat ataaacctga
     2641 tgaacttcga aaatcaggaa tgcccgatgc tgtagatact ccagctccac caaaaaaaac
     2701 aatgttgttt gaactttcaa taatctcttt taatctttct attttatcca taattaaccc
     2761 tcctgtttaa ttatatcata tttcttttaa ataataaaaa aagcattaat tagatatcta
     2821 attaatgctt ataatcttat tttttatctt ttttagcaat acttcttgca atatgaattc
     2881 catttgctcc tgcttgagcc aagcctctag ttagaccagc accatcgcca gctacatata
     2941 aatttttaat ctttgtttca aaattacgat ttacttcggg acgagctgaa taaaacttag
     3001 cttctactcc atagaacaat gtatgatcgc tagcaatacc tggagtgata tgatttaatg
     3061 catccatcat ctcaataatt gacttcattg tattataagg taaagctagt cctaaatctc
     3121 caggaacagc ttcttttaat gttggacgga taaatccttc tttaagacgt ttttttgtag
     3181 agcgtcttcc tctaataata tcaccatatg tttgaacaat tactgatcct gaacttaaca
     3241 tattagcaag tgttgcaaca gcgtgagcaa aatcatttgg attattaaat ggtttttcaa
     3301 actcgtgaga aactagtaaa gcaaagttag tattttctgt accatatcta atatctctaa
     3361 atgaatgtcc attacacaaa attgccccgt tttggttttc cacaacaacg tgaccactag
     3421 ggttagaaca gaaagttcta actgttgttc caacacttga acgatagata aatttacctt
     3481 catataaatg tgtatttata tcttccataa cgatattgct tgtttctacg cgaacaccga
     3541 tatctacttg gttattttta aatttaatgt cgtgtttttg taagacttct tcaagccaac
     3601 tagatccgtc tctacctaca cctaaaatga cattattacc atagatctca gttccatcgc
     3661 ataaaacaac accaacacat ttatcatctt caattaataa atcgccaact ttagttctaa
     3721 aacgatagtc gatatggttt gccatttcat cgcgaaagtt tttaagaatt tgaatatttt
     3781 cttcggttcc taaatgtctt actttacttc ttaataattt taagccagct cctattgcta
     3841 acttttcaat ttttctcacc tcagatgtat aaggatcagt tatctctgga tttgcaccat
     3901 gttctaagtt taatttatca acataattaa ttaattccat taattcatca tcatctaaat
     3961 actcatttaa ccaaccacca aattcattag ttatattata ctttccatcc gaataagctc
     4021 cagctccacc aaaaccagtt gagatggaac aacttggttt acagccgtga tatccgtttc
     4081 tatctactgg acatttttca attttacctt gtaagattgg acattgacga tcataaatat
     4141 catgtccttt atcaattagt aatactttat attctggctt tttaagcatc aattcatagg
     4201 cacagtacat tccactaggg cctgcaccta caataataca atcatatttt ttactcaaaa
     4261 tcttcacctc ttgttttcat aacacgcttt aataaattct ccaaataatg gatgtgctct
     4321 atttggtctt gatttaaatt ctggtcgata ttgaacagca ataaagaaag ggtgtccttt
     4381 taattccatt gctactggtg tgcctttttt agaaaatcca ctccaaacca aatcaccatt
     4441 tttaaattcc tctaaatatt ttttattaaa ttgatatcga tggcgatatc tttcactaac
     4501 ttctgtcttt tgataaacat cataaatacg agttccattt gttatttcaa cttcactcga
     4561 acctagcttc atctttcctt cttttcttaa agtaattact ggcattttac aatcaggatc
     4621 aagctctaaa gaattagctt cttttaaatt taaatttgat cttgcatatt cgataattgc
     4681 agcttgcatt cctaacgaaa tgcctaaaaa tggaattttg ttttctcttg cgtatttaat
     4741 acttagcatt aaaccttcaa ttttctcttg accaaaacca tcaggaataa taataccttg
     4801 ataagcttgt aatgtttttg ctacgttttt ctctgttatc ttggtagaat caattgcatc
     4861 aatctttact ttaagggaat tattaaaacc accatgagat aaagcttcat aaacagataa
     4921 atacgcatct tttaaagata catacttacc aattaaagca actttaactt cttcttttga
     4981 attgtttatt ctcgcaacca tttgatccca ttcttttaaa tcaactggtt ttgtttttat
     5041 tccaaaatga tttagaacta aagtatctaa tttttgttct tttaataaat aaggaacttc
     5101 ataaatagac ttacagtcag gaacatcaat aactttctca tcctttacgt cacagaataa
     5161 tgcaatcttt ttcttttgtg attcaggaat tggtctatct gatcttaaaa caataatgtc
     5221 aggaataata ccaattgatc ttaattcttt aacgctgtgc tgagttggtt ttgtttttag
     5281 ttctttcgca gctgtaagat aaggaactaa tgttgtatgg atataagctg tattttcata
     5341 tcctaattct ctacgaactt gtcttattgc ttccataaaa ggaagtgact caatgtcacc
     5401 aacagtacca cctatttctg taataacgat atctgcttta gatgtttccg ctgcttcata
     5461 aattaatgtt ttaatagcat tagtgatgtg aggtattact tgaattgttt gtccgtcaaa
     5521 ttcgcctcga cgttcttttt caataacact taaataaact ttaccagaag taaccgttga
     5581 gtgtttagat aaattaatat caatgaatct ttcataatgg cccaggtcta agtctgcttc
     5641 agcaccatct tcggtcacaa aaacttcgcc atgttgcaat ggtgacatgt ttgtagggtc
     5701 tacattaatg taaggatcaa atttttgaat gaaaactttt agacctctgt cttttagtaa
     5761 tcttcctaaa catgctgcat ttagcccctt ccctaaactg gacacaactc ctccagttac
     5821 aaaaataaac ttagcgcgac tcattttata cctccaccaa aaataaaaaa tagccctctt
     5881 cttttaagag aactattttg ctctttatta ttatatcaaa tattaaaact tttgtctata
     5941 actattttta gaattgtttt tgattatata agatcctcaa actcatcgtc ttcatcttca
     6001 tcataatctt catcatctaa atcttcgtat tcgtcataat cttcatcgtc ttcttcttcg
     6061 tcatcttcat caatttcatc agaaatgtcg tcatagatta cttcgtcatc gccttcttcg
     6121 tcatcttctt ctaatacttc atcttcatat tgagctaata actcttcttg catttcgtat
     6181 tttaatgcat cctcagagaa aacttcatca acaacatcaa tatatgtctt atcttgattt
     6241 agtagtttag agttttggcg atatttcaaa tcccataatc ttgtgccttc taagtcttct
     6301 gcacaacaaa tgaaattacc acttaacata aaatcaattt caaattgaat atagtcttga
     6361 ggatctactt tttcaattcc ttttctttta aatacttctg cagctatatt tacaatcgta
     6421 tctggctttt ttgtttcttc taaaattttt aaagctacat ctaccattga catatcttca
     6481 taaatatttt tatctttttc catcttttac tcctcctttt aaaagattgt atacttcttg
     6541 gttagtgcga tctccactta aaggtgtgat tgtgacatac ccacgatttg ttaaataggc
     6601 atcgctattt tcattattat ctaaagctaa gttgccggtt cctaattggt aatataatcc
     6661 ttctttttct tcaaaataag tatcaaaaca agtatcccct tgaacagcta gttttatacc
     6721 ttttggttct atcggaaaat ttacattata tactaaagct ttattccaat attcttcgct
     6781 taatagtaat tccataactt catcaaaata tttttcaaac cctgctagtg atttataatt
     6841 agctgataaa gcaaatcctc ttctttttgt ataagcagct tctgtggccc cagctacagt
     6901 tccagaataa aaaatatcaa aacctaaatt atatccttta ttaacaccag acagcactaa
     6961 atcaaaatca cattttaaat gataatgggc aaaacgaaca caatcagctg gagtgctatc
     7021 aatagcatat gcttcaactc caggaaaaag atttattttt tcaacactaa atcctcccct
     7081 tacaagtatt ttatggcttg tagctgattg attatattta ggagcgacta caaaaacttc
     7141 tccatatttc ttagttcttt caactaatcg ttttaaccct tctgattgaa ttccatcatc
     7201 atttacaact agtatcttca agttacatta atcctccgtt ttcctcttta aattctttta
     7261 atctaatatc taattcttct aaaattgaat caatttcttt ttctgctaat aatgtagctc
     7321 cgcttgcttg cttatgtcca ccaccacgat aattattagc tatatcgtcg atagacacat
     7381 atctacttct aattctagct ctaattgtac tatcgtcttg ctctacaaat aaaacccaaa
     7441 ttaaacttcc tttaattgag tctaaacaat taactagtga tgcggcatct tcaatagtaa
     7501 tattatactg ctttctgatt tttttatcaa tataaaaata agccacgcca ttttctgttg
     7561 ttttaaaatt taaatataaa taaccctcta attttaaaac ttccgagtct tttaaatata
     7621 gagcattata tattttttca aaatcgatat cataatctaa taataagcta gctgcattta
     7681 atgtttgtga tctaacattg ctatatttaa atctgcctgt atcagtaact attcctgtaa
     7741 ataaagcttc tgccccttct tgttccattt ctaaattttt cggtggatat aaaaagaggt
     7801 ctacaataat ctgtgccgtt gcaggcgcag taacgtcaac aaaattaatg tctgcataat
     7861 catcaacagg cagatgatga tcaattttaa tcgtttcttt tgcaagatga aaatatttac
     7921 tatcaattct ttctttagta gctgtgtcta agacaataac taatgactct tgatatattt
     7981 ctggttttaa ttcttcatcc ctcggtttta aaaaatctaa ataactagct gaatcatttg
     8041 caggtgcata gatctttttc ttaggaaaag atgttgttaa gatatttttt aaccccaaag
     8101 tagagccaac acagtctcca tcaggtcttt gatgacctaa gataataata ctatcatatt
     8161 ctttaatttt ctttaaaatt tggtttcttg ttttcttcat tttttactcc ctgcaactag
     8221 tttattcaaa tctttaataa tctccctaca aacttcaaaa cttgcaactt tagctccact
     8281 tgcaagtaag tgaccgcctc caccatattt aacagcaatt tgattaatat tatgttttga
     8341 cgaacgaaac tcacataaaa tttggttatt ttctgcatct tctacaaaca ttgcccaaac
     8401 ttcaatttct ttaatgttgg ccatgatgtt tacataacct ctagctatat tatgtggcat
     8461 taatccatat gcttctacat ctttttttgt tacttttaaa taagctactt tgtgtttact
     8521 aactttaaac ttatttgtta gtttcgctct taatttaacg ctgtctaaac tttcttgata
     8581 taagttagaa taaacgttat caatatctat atcatattcc attaatttag atacaatatc
     8641 aaaagttctt gctgttgttt tatcatatct aaaacgacca ctatcagtaa ctattcctgt
     8701 aaataaatgt gttgctgctt ccttgtttag tttccaatct aaatccaaag ctatttgagc
     8761 aataatccca gcacagcttt caaaactagt atctgtataa tttaaaacac aataatcgct
     8821 aactgtattg tgatgatcta tttttataga aaattttgct tttttataat tatcatttaa
     8881 taacatctca tcagtagaag tatctaaaac aaaaactaaa gcttttttat acaattcgat
     8941 atctatttca tcagttttgc ccataaaatc aaaacgctca ctagtatcac caactgcgta
     9001 aattttcttt tttggataat tttcttttaa aatagctttt aatcctaatt gacttcccat
     9061 agcatctccg tcaggattag catgtctttc tataataatt aaatcgtatt ggattacttt
     9121 ttcttttatt tgattaaagt tcattgtttt ctcctttatc ttcggaaact aattctagac
     9181 ttgaatttaa gtgataatga attgttagat atttgtataa accattacca gcaatcccta
     9241 ctattatact agtaactaaa gatattaata ctaataatgc tccagactta aaaaccgcat
     9301 cccctgtatg atatataagc attactacaa acaattgtgt aacaatatga agaaaaccac
     9361 caattacact tatacctaca agcgaagttt tatcttttaa taatttatga gctaaaaaca
     9421 tccaaaagaa actaactagg ctggcactag ctcctataat aaaagaactt atgcttccta
     9481 aaatcaatcc aactacaaca gattttaata cagtaattaa caaacttgtt ttaaaatcaa
     9541 aaatataaat agccagtaat ataacaatat tagcaagccc tattttagca actggtaaaa
     9601 aggggaaagc aataacactt attgatttat caaaaaacga caaaacagca gccattgcta
     9661 ccataattga tgctatcgca agttcttgaa ctttgtttac tctattgtga agcatataat
     9721 cacctttcct catcttccct tattttatca caaattattg ctttttttaa gatatttgat
     9781 ataatttaca taaggagttg atgttatgtg tatattttgt aagatagcta ataaagaaat
     9841 tccttcatat attatatatg aagatgataa gtttttagct tttcttgatt tatctcaaat
     9901 aacgattgga catactttag ttgtccctaa aaaacacttc gcaaatctat tagagcttga
     9961 tgaagaaaca gccaaggaag ctttaaatgt aacaaggaaa gtagctttat tattaaaaga
    10021 aaaactacat gttttcgatt ttaatattgt aaataattgc ggagaaaaag caggacaatc
    10081 ggtccatcat tttcatatcc atattatccc aatttacaaa aaaggcgacc ttgagtttaa
    10141 tactcaatct aataaattaa gtgaagcaga atttaaaaaa ttacataata aaataataaa
    10201 ataggaagtt taatcttcct atttttatat attagaaaaa taaattaata ataaagtatt
    10261 agctaatatt cctccagcag ctagatataa agacaacaaa gaaaatttgt atttcttttt
    10321 aatctctaaa ataatcgcaa ttattaaggc tacgcctcct aaaattagag caacaatagg
    10381 tacatatgga ataaaggcaa caaccattaa acttacactg atgattgtaa gaataaagtt
    10441 aatagtgtca aaactatttt tttctctttt ccttttttca acagttgtgt atgcttgttc
    10501 ttctgcaaca ttaacttgtt ctgcttcttc taataaattg ccacaaacat ggcaaaatat
    10561 agcgtcttct tgaacttgtg ttccgcactt tctacaatac atatctttat ccaattttat
    10621 ctaccctcat ataaggtttt aatacttctg gtactttaat tgtgccatct tcattttgat
    10681 agttttctaa aatcgcaact agtgtacgtc ccacagctaa acctgatcca tttaatgtat
    10741 gaacaaattc taatttacta tcttgtgatc ttctaaactt aatgtttgct cttcttgctt
    10801 gataatcttc agcattacta atactaccaa tttcacgata tgtgttttgt gatggaatcc
    10861 atacttcaac atcatatgtt ttacacattg caaatcccat atctccgcta gataatacaa
    10921 caacacgata tggaagtttt aaagctttta ataccttttc tgattcagca agcatttcat
    10981 ctaattcttg ataactatta tctggagaag ctatcttaat tagttcaact ttattaaatt
    11041 ggtgttgtct gattaaacca cgagtatcac gacctgcgct acctgcttct gatctaaaag
    11101 cagttgtgta actagttaat ttgattggaa gtttcgcata atctaaaact tcttctttaa
    11161 acatattgat tgttggtact tctgctgttg gatttaaata ccaatcacca ttaccatttg
    11221 caattttaaa agcatcttct gcaaactttg gtaactgtcc agtaccaaac attgatgctg
    11281 ccgatactat gtatggtggt attacttctg tatatccact ttttgcatgt aggtccatca
    11341 taaacttaat taaagataat tctaaacgag ctcctaaacc tttataaata gcaaatctac
    11401 tacctgtaac tttagctgct ctttcaaaat ctaaaatatc taaagcttct cctaattcat
    11461 agtgggcttt tggtttaaaa ttaaacttag ttggttccat aaactttttg atttctttat
    11521 tataactatc gtctggtcca attggtacag atttatgaac taagttagga gttgtagcta
    11581 gatagttaaa gattttttca tctaactctt taattgcctc atcttttgct ttaatctcat
    11641 ctcctaaaga agcaacttca gctaaaacac tactagcatc ttttttttct ctttttaaga
    11701 ttcctatttc tttgctagca tcatttctca tcttttttag tttttctact tctgtaatta
    11761 aatttctttt ttcatcatct aatttaatta catctcttaa gtatgaaaaa tcagaacctc
    11821 tttgatttag gtttttaatt acttcttcta agttttctct taagtattta atatctaaca
    11881 ttttcatgcc cccttaaatt tagtttattt taactctata agatatctat ttcaactaaa
    11941 aaaagccata tttcaacttt ttagtttttt tattttctaa acttaattat actataatat
    12001 ccttgttttt gcaatcaatt ataaaatatt aaaaagcacc cgaaggtgct ttttctttat
    12061 tcatattcta caaacaaatc tagatctgaa tctatgtctg cttctgattc ttcttttggt
    12121 aagatttcta ttgaggctac tttttggttt gcgttaagtg taataatctt agctccttga
    12181 gtaattctac cgattgttag aatttgctct aatggaactc taataataaa accataatta
    12241 gtaataacaa ttaagtcttc ttcagattca acagtcttca ttgctttcat ttgaccattt
    12301 ctcttattaa tacttaaagc tgttactcct tttccgcctc taacttgagt tctaaactct
    12361 cgtacgcttg ttctcttacc ataaccattt tctgtaacga taacaatttc atcatttgga
    12421 ctattaataa tagccatacc aactacttct tcgccctctt caactaaaat gcctcttaca
    12481 ccttgagcta ctctttgcat tgggcgaaca tcatcttcgc taaacttaat tgctttacca
    12541 tttgatgctc ctaaaataat gtttttattt ccgtcggtaa taccaacact taataattcg
    12601 tctccttcaa ctaaattaat cgcaataata ccattacgtc taatgttttt gtattcttca
    12661 acttgtgttt tcttaactag tccttgtttt gtagcaaaga ataagtaatc tgtttcttta
    12721 atagcatcag tatcgatact taaaacagtt gctagtcttt cattattttc aagtttaagt
    12781 aagtttacaa tcggtgttcc tctagctaat ctattagctt cggctatttg ataagctttt
    12841 aaagtataaa ctttaccaaa gtttgtaaag aagaataaat catcatgagt tgaagtatag
    12901 ataacttttg taactgcatc tgattctacc attttagttc cgataatacc ttttccacct
    12961 ttgttttgtg tttgatatgt atctgatgtc attctcttaa catatcctct ttcagttata
    13021 gtaataataa catcttcaac tggaattaga ttttcatctt ctagccctaa atcttcagct
    13081 aaactaattt ctgttcttct tggatcttta tatttttctt ttgtttctgt aatttcttta
    13141 ataattagtt cttttttctt ttggggattt tgtaagattt ctttgcactc agcaataaat
    13201 ttttctaact ctacaagttc ttctttaatc ttttcaactt ctaaactagt aagtctttgt
    13261 aatctcatat caagaattgc tttactttgc tcttcggtta aaacaaattt ttccattaaa
    13321 cgattaatag caatttcccc actcttacta gctttaatta ctttcacaac ttcttcaatg
    13381 tttgcaagag caataattaa tccttctaaa atgtgagctc tagcctctgc tttttctaag
    13441 tcaaattctg ttcttcttct aattacttcg ttttggtgag ccaaataaca ttctaaaatt
    13501 tcttttaaat ttaaagtttt tggttgtccg ccatctagaa ctaacatatt ggtactaaaa
    13561 gtagattgaa gttgtgtgtg tttaaataaa ttatttaaaa caacacccga attagctcct
    13621 cgcttaagtt caattacaat tcttaatcca tctctagtag attcgtctct taaatctgtt
    13681 atatcttcaa ctattttatc tttaacaagt ttaacaattc tatctactaa aagagtttta
    13741 tttacagcat aaggaatttc agtaacaaca atttgttggc ggtcgcgacc taaatcctca
    13801 taatgcgttt tagctcttac tactaaagat ccttgtcctg ttttatacat ttcacgaata
    13861 gcagttagcc ccattaagat tcctcccgtt gggaaatctg gtccttttac atattccatt
    13921 aactcttcaa ctgtaatctt tggattttta actacagcta gtaaagcatc cattatttct
    13981 tctaaattgt gtggtggaat gtttgtagcc ataccaacag caatacctgt tccaccatta
    14041 actaacaaat taggaaaacg agatggtaaa attacaggct ctgtttctgt accatcatag
    14101 ttatcttggt aatctactgt attcttgttt aaatcacgca ataactccat actaattttg
    14161 ctcattcttg cttcagtata acgcatagca gctgcttgag caccaccaat tgaaccatag
    14221 tttccttgcc cttcaactaa aggatatcta aaactaaaat cttgagccat tcttaccatc
    14281 gcatcataga ttgctgagtc accatgaggg tgatatttac ccataacatc cccaacaatt
    14341 cttgcagatt ttttatatgg tttatctgaa taaagtccta atccatgcat tgcataaaga
    14401 atttttcttt gaactggctt taacccatct cttacatccg gtattgctct tgaaacgata
    14461 acactcattg cataacttaa aaatgagttt ttcatttctt ttacaatatc aatgttcttc
    14521 acgccacctt tatgatggta ttgtttttca ctacttgttt cttcttcaat tgggctctct
    14581 atatcaataa tatctttttc cataagagtc ctcctatacg tctaaattct ctacgtagat
    14641 agcgttgctt tcgataaatt ctcttcttgg cgcaacatcg tcacccatta gcatactaaa
    14701 taccgtatca gctacagcca cattatctaa tttaactttt aataatgttc tggttttagg
    14761 atccatagtt gtttcccata attgatctgc attcatctca cctaaacctt tgtatctttg
    14821 aatatttggc ttagcattaa atgtttctaa aacatcttgc aattctgctt cgctatatgc
    14881 atattctatt cttttaccat aacttacttt atataaagga ggttgagcaa tatatacata
    14941 ccccgcttca attaaaggtc tcatataacg ataaaagaaa gttaataata gtgttctaat
    15001 atgggctccg tcaacgtcag cgtcggtcat aataactact ttatgatatc ttacactttc
    15061 aatatcgaat tcttttccaa tccctgcacc aattgcttga atcatagaga taatttcgtt
    15121 attacttaca acactcgctt tggttgcttt ttctacattt aacacttttc ctcttaaagg
    15181 tagaattgct tgaaattctg cgtctcttcc catttctgca ctaccaccag cagagttacc
    15241 ttcgacaaga tataactcac ttattgttgg gtctttagaa cgacaatcac ttaacttaga
    15301 agcaaagcct attgtttcaa gtgggctttt acgagaagct tctctagctt ttttagcagc
    15361 cagtctagct cttgatgatt ctaaagcttt attaataatt tcttttcctg tttctggatt
    15421 ttcgtctaaa tattgttcta attgatcacc taagatttgc gatacgattc ttcttgcatc
    15481 tgcatttcct aatttagatt ttgtttgtcc ttcaaattca ggcgaagggt gtcttacgct
    15541 aacaatcgca gttagtcctt ctcttgcatc gtctccagtt agcgactctt tctttttgaa
    15601 aagacctatc tttttaccat attggttaat gatttgcgtt aacctagttt taaatccttc
    15661 ctcatgagtt ccaccatccg gtgtattaat gttatttgca tatgagtgaa tacggcaagt
    15721 ttcatcatct gtatattgca ttgcgatatc tacttctaca ttatctactt tccctgaaag
    15781 ataaataatc tcattatgaa ttggcgtttt actttggttt agatatttaa catattcaat
    15841 aataccatta tcgtattgat atgtattagt tctaacacga tatgggttac gaaggtctgt
    15901 taatgtcagt ttcaacccct tgtttagaaa ggctgattgt tgtaaatgag aagctaatgt
    15961 ctcataatca aattctgttc cttctttaaa gattgttgcg tctggtttaa aaagtactac
    16021 agttcctgtt ttatctgtaa ttccaattct cctaacatca tatagtttct ttccgcgacc
    16081 atattcttgt tcgtatacat aaccatcttt aaatacagta acttttaacc attcacttaa
    16141 agcattaact actgaagcac caaccccatg taatccacca gaaaccttat aactctttcc
    16201 gccgaattta cctccagcat gaagaacagt aaatactgcc tctaatgctg attctcctgt
    16261 tttgggatgg atatctactg gtatacctct accattatcg gtaacagata ttgatccgtt
    16321 tggttgaatt tcaacttcaa tatgatcaca ataaccagct aaagcctcat caattgagtt
    16381 gtctactatt tctctaacta agtggtgtag tccagtatca ctagtactac caatatacat
    16441 accaggtcta gttcttactg cttctagacc ctctaatatc tgaatatcgg agccactata
    16501 ctgtgaattt aatcttttat catcactcat cttattattc cctttctatt ttaataatat
    16561 tgctattttc caataatgtt tcgtgtattg aatcaattgt tgttgtagta ataaatattt
    16621 gcttgtcttc tttaaacaat tgcataattt gattttgtct ttctttatct aattcactaa
    16681 aaacatcatc taagataaca atgttgtcca ttcctaattt atcaaacaag ttggcaagtc
    16741 ctaattttaa agctatgctt gatgttcttt gttgaccttg tgaggcaaaa gatgctgcat
    16801 ctttgccatt tatttcaaaa ataaagtcat ctttatgtgg tccaagccct gtagtttgca
    16861 aatataaatc ttttttaagt tgtcgaaaag ttttcaacaa aaaatcatct ttttccacat
    16921 ttggcatata ttttaattct gttttctcgg tttcgttaga gatagccaaa ctaatttttg
    16981 ccacttcggg atttaacatt tcaataaatt tttttctttc aataattaaa ttaattccgt
    17041 attttactaa agcttctgag tagattttta atacttctag atcttgttcg cttgggttcg
    17101 gcttctttaa taattcgttt ctattcttca atactctatt ataattagat agatctttta
    17161 aatacttgtt attaatttgg cctaaataaa catcaagaaa tcttcttctt tgaggcgaat
    17221 aaccttttat caactcagaa tcatcaggcg aaaacataac gactctaaat gttcctatgt
    17281 aatcactcat ttttttaatt acattattat caactagcaa tcttttgcgt aatttatcta
    17341 actcatatga caaaagcaca ttatgactta tcgctttgtt tataaattct ccttctaaaa
    17401 catagtatgg ttggtcgtgt tgaattaaaa gagaatcacg gcttgcttta tatgatttag
    17461 taagacttaa acaataaata gcttctgcta aagatgtttt accaactgcg ttttttccca
    17521 ctattatatt aacaccaggt ttaaaaatta tttttttatc tttataacaa cgaaaattga
    17581 ttaattttaa tttttgtata taaaacattt tttactccac tacttatatt caagctttat
    17641 atcattaaaa caacaaattt ataataaaaa cctcatcaaa atcatagctt ttacctattt
    17701 ttgttgcttg gataatacat agatattata ccatatttca gcctattttt aaagactttt
    17761 ttgaaaatct ttctgattat attttttaag ccaaaaaact cgcttatttt cggtttaggt
    17821 gcgtttttct ataaaatatc agtttaatta agtcttttgt ttaaaatttg tttaagttga
    17881 aatataaaac ttaaaaaact aatagtttct ttgttttaat aatatattta gtagttagtc
    17941 atgtgtactt ctttgcataa tgttgttttt tctccaattc tattgaaatt aatttttaga
    18001 gatcaataag ttatttaaaa aaacaacctg ttttttgcac gcatatataa aattatttca
    18061 aatttaaatg tttttgggtg atagttgaga tggttttgtg tattcttata ttgtttatac
    18121 taataataaa ggcaggtttt tggtctgaat atataagttt acttaaagaa gcgattcaca
    18181 taagcttttt tggtatgatt ttatgcgtaa acagcactgt ttaatcatag aaaaagtcga
    18241 ttttttaata attttaaaaa tttagtcaat acatgaacag atttatgtgc caaaattaaa
    18301 aaaaataagc tattaacaat ctttttagtt taatatttga tagttttatt ttaaaactaa
    18361 agatttttcg tttttcggac tgttttttga gtttttttgc atctgaaatg attatttagc
    18421 ctgttttttt tgctgttttt tagttttaag acgtgtaaaa acgcaaattt tacagtgttt
    18481 ttgcttgaat ttctaattta aacttatata aaaaggctca aaaaaggagg gttaataatt
    18541 aaattatctt ttttgtttgt ttttctcgac tcaacgcccc aaaaaacata cattttttaa
    18601 atttttatgg ttttatgtat tgctttttgg catttttttg tgtataatga agtcaacaag
    18661 ttaagaaaaa acaaaaaaac ttgttttttt aaataattcg gcgtatggtt ttatgtataa
    18721 ccgtaaaacc atatatatgg gtttatggtt ttatgtataa tttgggcata tttaatcatt
    18781 aaacatctca aatacgacca aaaacccatt ttcgcaacct ttcaaatttc gtttttaagc
    18841 ctttttaatc tccgacctta ataaatactt tactacaagg taaaaattga gcttaaatta
    18901 aattcaaaat gttttttgag gtgctgaaaa ttatttctct ttttctaggt tttggttttt
    18961 agaaattgag gaaaatttgg gtttaaaaag ttaaaaatcg tgcctaaaaa gagttaaaaa
    19021 atgtatgatt ttatgtataa acatataaat gtaaatatgg ttttacataa aattgtataa
    19081 atgtataaat ggttatatgg ttatgtgtaa aaatgtaaat gtggttttac atttttaagt
    19141 aaaaaattat aaaattgtaa gaaaaaggag taaaaatatg atggataaac ttaaaaaaag
    19201 agcctctagc cctttatctg gcctagataa aacagtaaat actgctacaa ctgttagtga
    19261 agagtctcaa cctgaaccaa ttgttcaaga accggtagca tacaaaccag agccagcgcc
    19321 tgccacacct aagcaaacaa cccaaaaaac cgctaaaaaa ggaggttttg aggtgtttgg
    19381 tggcgatgaa aagataaaat atacagctac tatgtcccat gaggtaagag caaatgctaa
    19441 aatagcagca atttctttag gttggacgtt ttctgagttc gttgaagagg ctgttagggc
    19501 ttatttgctt actagaagga taaaatagga tcattatatg gctattattt caacatattg
    19561 taaaaagggt ggcgttggta aaacaacttt tattggctat ttagctcatt atttaacaga
    19621 agtaaaaaat aagactgttt taattatcag tgttgacgat caaaacagca tttttactct
    19681 ttttgggaag caaaatttag ttacagcaag taatgatgac tatttagaac acctaatggt
    19741 gggtcttagg acatatgaag aggtactaca cgaggtaaga gataacttat atttaattaa
    19801 aaccttaaat acagatgctt tatctactaa attaataatc gatagaagca gtgaaaagac
    19861 tctattagag ataattgata cttataacga agcttttgat tatgttttgc ttgattttcc
    19921 tccttctagt tgtagggcta cagaagtttt attggattat agtgatagaa ttatgttaat
    19981 tattagttta gatagtttgg gtattgatgg ttttattaat acaattcagt tttttgtcga
    20041 ccaagacatt ccaattagta aaatacagta cattttacct aataaattca tcgcaaatag
    20101 aagagcacca aagaagtcat tagaaacatt acaagcacaa gctgaagagt tttctaaaac
    20161 tatggagttg gacgctttga ttttgcctta tttttcagaa aaatccactg ttcaaaatat
    20221 tcaagaacaa ggaaaaacgc cttttgataa agatgagttg tttacttatc gctatgattt
    20281 taatgaaatg ttaaaagtga gagaagaatt acttggagtt tttaaagctg ttgatgaata
    20341 cgaagaagaa ttagcaaaca gagctaaaaa aggaaaagaa aaagaaaaac cagggtttca
    20401 acttaagaaa aagaaaaaat aaaaagaggt ttaatgacct ctttttttat atgtttgttt
    20461 ttttattaag atactcttac tggtaatatt agttgagtta aattaacatc atattcacca
    20521 gtaacaataa atggtttaat ttcccctgta aaatgaacta caatttcaga agaatcaaac
    20581 gatcttactg cttctaagaa gtatttagca ctaaacgcaa tcttaaatga aaccggatta
    20641 gaacattcaa gtggaattaa ttcttcttta ccttcaccaa tttcgctagt tgttgatttg
    20701 atttcaactg ttttgtctgg atttaattgt aattgaataa tgtttgaaga ttcaatatta
    20761 gtaaataaag acattaaatc aatagtttta acaagttcag ctttattaaa cttaatacta
    20821 gttaaaaatt ctgttggtat taagcttgat gtgtttggat atacaccatc aattaatctt
    20881 gattggaata acaagttttt gtatttaaat aagatctttg ttggtgaaaa gtgtaattca
    20941 atatcacttt cgttgtcatc aataataact ttgtttaatt catctaaact cttagaagga
    21001 ataactacag atacttcagg taattcttcg ttaacattta agtagatttt tgctaatcta
    21061 aagctatctg ttgaaatggc ttctaattta ctgttagata agtttaaaca aacacctgta
    21121 agaattgctc ttgattctga taaagatgta gcaaaagtag tttttctgat aatttgtttt
    21181 agtgttccta tatctaattt gataaatgat ttcgattcag caaatgaaat taacgggaag
    21241 ttgtcttttt ctaaagcatt taatgtaaaa tcacttctat ttgcataaat tttaattacg
    21301 ttatcttcta ctgctttaaa ttgaacttcg tttgcgtcta attttctaat gatctcgatt
    21361 agtattttac ctggtactac aaaacttcct tctccttcaa gctttaactt gtttccttcg
    21421 tttatttgtg cttgaattga gatttcgtta tttgaagcgg ttaaaaacat tttgcttttt
    21481 gttatttcga atttaattcc tgttaataca ggcattggtg ttttcgttga tagagctcta
    21541 cttacgtttg ttaggttctc taacaataaa tctctgttaa ttgtaaaatt catattttta
    21601 tttctccttt ttaattttaa ttttatgttt ttattattaa catatgtgga tatgttgata
    21661 actactgttt ttcaccttga attttatgag ttaaatacaa agcatcttgc tttatacctt
    21721 tattttgttt catgtctttt tcaatcttgt ctatagaata agatatagtg gcatgatctc
    21781 tatttccaaa atagcttccg atttgtttta aaggcaagtc ataaagagtt ctgattaaat
    21841 aaacagccat ttgtcttgca aaaactattt tagcttttct agaaccagat tctagttcag
    21901 gaatcgaaac ttgataatag ctagcaacag cttctttaat atctctnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnctag ttcaggaatc gaaacttgat aatagctagc
    22081 aacagcttct ttaatatctc taaccttaac atcagccgga gctccttctt tatctaaacc
    22141 ttcaactagt ggttttagta taggatagac attttcttta gtgaatggta agttacctga
    22201 tacgcaatac caaatatatc tacttaaagc tccttctaat tctctaacat tacgatcttc
    22261 ataaatttcc gcaatcaact ctaaaacctc aagaggaacc tcttcagggt ttgggatatg
    22321 agtaggaagc ttcttttgta agatacctaa tcttagttgt ttatcgggtt ttttgatatc
    22381 tacagctaaa ccatagctta atctactttt taatctatcc atgaagtttt gaagttgatt
    22441 agctggtcta tctgatgtaa ctaccatttt tttcttttct gtatataaaa gctcaaacaa
    22501 tttgaaaaac tcttcttgag tttttgtttt ttgctctaag aactgtatat catctactaa
    22561 aagaacgtcg gcagattgat aatagtcata aaacttttga aaagcttttg gatctttact
    22621 gctagatgca gtaaagtaat cttgggcata ttgttgcgct gtagtataga ctacattagt
    22681 atctggtttg atttgtaaga tttcattgcc gacagcaaac ataagatgtg tttttcctaa
    22741 tcccacccca ccaaaaatat aaagagggtt taaaacagga attggatcgt ttgctacttt
    22801 agtagcagca agaaaagcaa acctattact ttctccaata acaaagttat agaaggaata
    22861 ttcaggtagt aaagatcgcg gctttttgct attatcgata tctgtttgtg tagtatttac
    22921 taaatctact ttatttcttt gttcttctgc ttcttttcgg tcgataaaag caaaatggca
    22981 accaaaatta tttaaagcaa attcattgat cccgttttta taaaatttat caattctaaa
    23041 ttttgtaaaa atactatcga cgataatcca aattgtacta tctttaactt taaacacttc
    23101 tttagtgtta gaaaacaaat cattgtaaga aatgtcgtca atttgatttt ttaaatgttc
    23161 gattgtcttt tgccaaatat ctaaagcatt ttctacattt aaattattca taccaaactc
    23221 cttcccatat atgataccat aaaaagaagg aataataaac ataaaaatta aataattttt
    23281 tatctacttt tccacagtgc aaaaagcaag gaaaactttt taaaaaagaa taaacaattt
    23341 atcaatgttg aaatgttaac tgtaaggatt gaaaaaaaac aaaaaaatga tataataaat
    23401 aaagaggtga actcatgact aaaagaggaa ataacgtcaa agaagtaaaa aaaattacta
    23461 aaaagatatc ttctttatct ttgaaagtga taatttcagc tgttttagtt ttattaatct
    23521 ccctattgtt ggtagggtgt ttcagtaatc cacatattgt caaggacgta aaagttagaa
    23581 ttgataaata taatttaaat aaaaattcta gccaaacgat aatatatgag atagttcctg
    23641 aaactgctgc tccatataat aaagttactt ttacatcaag taacacagaa gtacttgtga
    23701 ttgatcaaga tggaaaaatg actgctctta aagaaggaga aacaattgtt acaattactg
    23761 ctggtaagat atctaaaaca tataaaatta ccgttttttt ccttgagata gatgacgaaa
    23821 tggaagaacc agaaagcgac ggattttata tgtatgttga agaagctgaa ggaacttatt
    23881 acgcttctgc taatggtatg gaaggagcgg atttaaaaac agaacttaat cgcatattaa
    23941 gagatggttt tacaccacga tcatatggag atgctaggga gattttaggt agagcagatg
    24001 ctgatctaga agatcctaca aaagtttgga caatttatag tggagcaaaa attaatgcaa
    24061 cttgggatgg taaaagttgg acaagagaac acgtttggcc tttttctcgc ttaggaacag
    24121 gaactagtaa tattggtaat agtactagaa accaagctag tgatttacat aatttaagag
    24181 ctattgttca aagagtcaat tcatcaagag gaaacgcata ctttacaaat ccaattacag
    24241 aaactacaac ttattgtgct gttacttcta actcttggtt tcctggtgat gatcataagg
    24301 gtgacgttgc cagaattctg ctttatatgg atgtaatgta tgaagaatta agtttagttg
    24361 aaacaggagc agagttacta actggtgaaa cctatcaatt atctggagct aagatgggct
    24421 tattacctat attattagaa tggcatcgtc aagatccagt tgatgatttt gaaattcaaa
    24481 gaaacgatat tatttacgaa gaacaaaaca atcgaaaccc gtttatcgac aaaccagaat
    24541 atgttcacct aatttgggaa ggtaaaacca ttaatgattt agtaaagtta tggtttaaac
    24601 ctatctacta ttataaggaa gaatatacat atgtgtttta ggaaaaagaa agaaataatc
    24661 ttagaatcag gctattacta tattggtgat ctttattata caattactgg gaaacccgct
    24721 agcaaaaaat tggaagaaaa actattaaag aaattttata aaaaaaataa acaattagca
    24781 aaattaaaaa aaattaaatg tcaaggaatt aagttatatg ctttttctcc aaacaataat
    24841 ttattgctat attcaaaaaa agaagttatt aatgaaatag ttttagagac aaaagtcatt
    24901 tgcattttaa acttaactaa atatggcaaa cacaagctat tccctttttc tagtttaagt
    24961 acagaaaact ttcctaattc aggatttatt tatttaaatg aagatactac tatagcagaa
    25021 acagaaaaag gtgatataat tataggtaac tatagtatta aagaaaaaac aaaaagaaga
    25081 acaagaagga agaaaaaaga atgaaattaa ttaaattccc agaaaaatta aacaatttta
    25141 caattttagc aatcttaatg ctttatgctg ttgcggcggt agtgcttggt tattttgcta
    25201 tacaaaacaa agaaattgtt actgctacct cttatgcaga acatgtagaa aacgaaaaag
    25261 gctattttaa ttttataaaa ataagcaata gacttgctaa agaagaaaat gtggaaggga
    25321 atcctctaac taaccgttat tatattactg gctatgtaag gggaacatca gcaataaaag
    25381 tagatctatt taaagcaagc tatagcgtat cttatgttga tggtaaagct tattattttt
    25441 ctactctaga tagttttagt cacactaata gttatgtgat ggcttcaaac agcaaaggag
    25501 cagacttaga agcatttact tctatcatcc atttttatga cgatgttgag gatgaaaacg
    25561 gcggcacaac aaaagaagag tatgtttata aatttacaga acctatgtta acttttgatg
    25621 aaaaagagaa aatcactgat ttagaagccg taaaagatat ctttttgtta aattttatcc
    25681 ccaagaaaaa aaccgggaaa acagattatt attttaactt agattttaga tctaaagttg
    25741 caaactatga atttgatgtc caagggtttg taaagaccga caaaggcgaa attttcacta
    25801 cagtaggctt atatcatttt gagggaaatt attacggaga agatataatt aatgctcatg
    25861 tattagaaaa cgaatatatt actgaagtta ctcatccaaa gactttatat tttaaagtaa
    25921 aatattatga taagataagt aaggaaacaa attattatct tttcgaaatt ccatttcctg
    25981 cagattttta tcaagaagaa agtgagtaaa aaattaattt tagaaaataa ttgacaatcg
    26041 ccgagaattt gtatataata acgaggcgtg gtaaaaaata ggaggtgaaa ttatgtcaaa
    26101 aagaacatat caacctaata aaagaaaacg aagcaaggta catggatttc gtgctcgtat
    26161 gtcaactgtt ggaggacaaa gagttttagc taacagaaga cgtaaaggca gaaaaaaatt
    26221 atctgcataa ataaactcta cgaccacttt tgtcatttta acgtaagtgg tctttttatt
    26281 tgttgataga ggtgtaaaat gaatcgtaaa tatagtttaa agaaaaatca tgatattaaa
    26341 gaattactgc agaagaaaca atctgttggt aataaatact atgtgatcta ctatgattat
    26401 atagaaaaca aaactcctct tatagctttg tctgtttcta gaaaagtagg caacgcagtt
    26461 gaaagaaatt atgaaaaaag agttgttcgt gaaattatta ggccgctttt agaaacaatt
    26521 gaaaactata agatgttaat tgtaattaaa gataaaagca aagaattaag ttacgtagaa
    26581 aaagaagaaa atattaatta tttaattaaa aaaatgaaaa cacaaaagga gactattaat
    26641 gaaaaaaatc ttaaacaaca aaacagttaa gatgataggg gctattgttt tagttttatt
    26701 tgtagcatta gtactaactg gttgtggttg ttcaggacaa ccaacagaag gcggggtagc
    26761 tgctccagac ccaattgtag gttttaagag tttttgggat ttatttgttt ggccaatggc
    26821 tgcaataatg tgggttgttg gtaaagttat gggaggcaat tatgggctta ctattatttt
    26881 tactactatt ttagtaagaa ctgcagcatg gccgatttat acaaaaacaa acgacatgtc
    26941 tttaaagaca aagcttatgg caccagatat ggaaaagcta gaagcaaaat atgcgggtaa
    27001 agacgacaaa gaatctcaac aaagaaaaca aatggaaatg atgcaactat acaagaagta
    27061 tggcattggt attggtggat gtttattacc attccttcaa atgcctttat tcttaggatt
    27121 cttccaagca ttaagaagaa taccagacac attaggagct gaatatccac tagactttac
    27181 ttttttaaaa tccaactttt taggattaaa tttatttgca agtaggacaa cggcgccaga
    27241 aatggcaact aaaatttgga tattagcaat tgcagtcgga gtacttcaag tattatcaca
    27301 agtactgata atcattagac aaaaactaca agaaaagaaa gtatattcag acgtaccaga
    27361 ataccgcaga cctcaacaaa atcaacaaaa caaatctcaa aatatgatga tgaacgtttt
    27421 tgctattgcg atgtcagtaa tgatggttgt gtttgtttta aacaacccag caggtttagg
    27481 tttatactgg ttagtaggta atatctatac aatgatccaa gcacaaatca gctatatgtt
    27541 aacagaaaaa cgattagcaa aattaaagga aaaattcaac aaggagtaaa cgatggaaaa
    27601 gaaaacatat gaagttaaaa gcattgaaga agcttatgaa ctagtaaaag aagaattagg
    27661 cctagataaa gatcaagtaa atatcgaggt agttgaaaaa aagggtttat ttaaaactaa
    27721 aatggttgtt gaagttagcg aaaaacaaga cccagttaaa attggtgaag aatatttaaa
    27781 agaaatatta aaagcaaaca acattaaagg tatagttgaa aagaaagtaa gagacaatct
    27841 tatttactat aatataaatg caggcgacga taatggttat ttgattggca gaagatctcg
    27901 ttatctaatt gctttacaaa ctttagtatc aacagctatt tacaatgtaa ctaaatcaat
    27961 ggatattaat gtaattgtag acgtagcgga atacaaacaa aaaagaattg attatttaga
    28021 aggtttagca gctcaacatg caagaagagt taaagaaact aaaaaaccaa ttgctttaag
    28081 taatttaaat gcttatgaaa gaaaaatagt tcatgataaa gttgcaagcc taaaaggagt
    28141 tacaacacat tctgaaggcg aaggcaaaga tagacatcta attatcgaac caacacgcga
    28201 ttaatacaaa caaaaaaacc acaatttatg tggtttttta ttatttaaaa tatttttcaa
    28261 aagaagcttc ttctaattga attaaatagt ttataaaagt aagtgttgtt ttcttaactt
    28321 tgtaatcctc caaacattga aaataatttt ctttatctgc agaaggaata atgattggtg
    28381 caagtccaga agccaacatg taatagttta atactagacg agctaatcta ccatttccat
    28441 ctaagaaagg atggattttt gaaagttgta aatgactata agcaatttta tttattggaa
    28501 aatcagcact ttttagaact tcaaaatatt catccatacg tgaatatatt ttataatgtg
    28561 gtggtggtgt gtgttgagaa ccttgaatag aaatattaaa agttctatat aaaccgccat
    28621 gttcatccat taaaattgaa tggcaatctt ttagttttga ttctgtaagt gattctccct
    28681 ttttagctag gtcaaccata aacaaaaagg ctttacgatg attttctgcc gttgtaacca
    28741 atttttcatc atattttgca tgattaccgg acaagatgtt tttgatatct tctctggtaa
    28801 cagctcttct ttcaaaagtt agcgagttgt aaacaagatc agtaatgaac tctaaatcaa
    28861 ttttatcgta tgaaaaacgt tcagccgcaa gctttttaaa agcttcatat ttttcttgta
    28921 aaaatttttc catataacct ccttagttat ctatactcca attataacac aataaacggc
    28981 aataataaag caaaatccga aaaaacttga aaataaaatt aaggtattgt ataattatta
    29041 caatagggga atgcgtatgt atgatgatgt tatttgtgca atttctactg ctaacacaag
    29101 aagcgcgatt tctattgtta gattaagcgg aaaaggctca atcgatattg taaataaaat
    29161 atttcgcggg aaggacctaa ctaaagtggc tagtcatact attcattatg gatatatata
    29221 tgacggcgat caaatgttag atgaggtttt agtaagcgtc tttaaagcac caagaacata
    29281 tactaaagaa gatgttgtag agattaattg tcatggtggc gtttttgtaa caaacaaagt
    29341 tttggaactt tgtattgtaa aaggagctag acttgctgaa ccgggcgagt ttactaaacg
    29401 agcattctta aatggaagga ttgatttaac tcaagccgaa gctgtaatgg atgttattga
    29461 tgctaaaaat aaagaaacat taaagatgtc taattatggt ttaagaggag atatcaaaag
    29521 aattattcaa gaattcaggt caaaaatctt ggagataatt ctaaaaatag aagttaatat
    29581 cgattatcca gaatatgagg atgaagaaca aataactaag gaatatctaa ttcctagtat
    29641 taaagagaat attgaagaaa tagaaattat tattgcgaaa agcgaaaatt caattaaaat
    29701 taaagaagga atcactactg ctattattgg aaaaccaaac gtaggaaaaa gcagtattct
    29761 aaatgcttta ctaagagaaa ataaagctat agtaaccgat attgctggaa ctactagaga
    29821 cgttgtagaa ggtagtgtta atattggcgg acttacttta aatttaattg atacagcggg
    29881 cgttagacaa actgacgata ttattgaaca aattggagtt cttaaaaccc aagaggtttt
    29941 agagaaagcg gagttgatta ttttagtatt tgattattct tctaagttag atagtaatga
    30001 tttaaagata attaatttaa ctaaagacaa acacgttcta acggttatta ataaaaaaga
    30061 tctaccacaa aaaatagatc ttagtttatt tgataattat ataattatta gtgcttttga
    30121 tgaaaaagat atcgaaacgc tagaaaaagc agtcaaaaag gtatgtgaca tccaacaaat
    30181 tgatgatagc gaagttaaat atgttacatc aataagagca atcacaaaat taaaaaaagc
    30241 tttgcaatcg ttaaaggaag gattaaaggc cttagagtca ggaatgccaa ttgatcttgc
    30301 aagtacagac ttgcgtagtg catggcaaaa cctaggagaa attttaggag aagttagtag
    30361 cgaggagcta cttgatgaaa tgttttcgca attttgttta ggaaaataag caaaaaaata
    30421 aggagggtat atgaaaacag aagcagtttt aaaaaagaat ctacttagca tcataggtgg
    30481 aggtatttta gtatcaggat taatagtggc ggttgtatca gcaatttttg gcggtggaat
    30541 accaggaatt ttgagtcttg tgtgtgtatt gggagcaatc tttggattag cattagcgat
    30601 agcaactgaa aagtttttat actttattat atcttttgta tcgctagcat tatttgctca
    30661 tacatacatg gatgttaaca atgtaattta tttgtttttc tttttatcat tacttgtagc
    30721 tgtagcagga attgtatatt atggtattaa agtaaaagga aaaattttaa cagcaattgc
    30781 agtatttgct ttagcggcag taagtttagc tttatttatt tttgcacttg ttcgcgaagc
    30841 tccagcagca ttcgtgtttg tagctgtagg tttacttcta tctttttctg gtcaaggaat
    30901 tattgttctt gctggagata gtaagggaga attttttgcc gagaaagtgg caaacttaaa
    30961 acaaaaagaa aatgaagcaa aagcaaaaga aaaagaagaa aaagaaaaag aagttattaa
    31021 cttagattga ttagataata aaaaagaccg aagctaaatg cttcggtctt tttatttatt
    31081 tttattctac ttctttatat atatatttaa acaaacgagt gtttttaaat atcaatggat
    31141 agatacctaa accaataaag ataactaaga agtatcttac catatctaat atcaaatatc
    31201 catcaggaaa aacaaacttt aaaccaagat aaaccacaag cataattaca ataccaattg
    31261 cagctcttaa gattttcatt ccaagcgttg ttagatcagt aaatttaaca agtttgtttt
    31321 caataataac tgcaccaaaa aatcctaagt aagctgcata agaaatataa aagtcgcgat
    31381 aaactaacat atcattagca ttaggtcgat aaaagaaata taagaaaggt gcaaaaatta
    31441 ctaaagtagt tgcgaacaaa ataattttcg atttgaatga atgttggaaa gtatcatata
    31501 acaaaccaca aagcagcgat accccaattc ctaaactaat tcctacaact acatcagata
    31561 aaaagtgaac tccaagccaa attctcgtaa acccaacaat aacaataatt gctattgcaa
    31621 caatccaaag ccaagttttt ttgaaatggt tggctattga aaaataaact gatgcagcac
    31681 cttgtgaatg gccgcttggg aatgaatagc ctgtggcatc ttctactccc gctgcttcaa
    31741 tatctggatc tacttgaaat ggtcttttaa ccgcgcatat tccttttaat gcattgttaa
    31801 cgccataaga agtaagggca atataaatta ttttttcacc agccttttta tctaaaacaa
    31861 aatatataat tgcaacaaga acaataatta ccatttgcga acccataaat gttaaaatct
    31921 gaacaatttt atctaaagtt tcggtactta tgctcctaaa aaaacgagaa atatttaatt
    31981 caaaattacg catctcaacc cctccacact ttattttaac tcatctttat tttaaagtca
    32041 atttaataat aaaccttgaa aaaaacaaaa taaattgcta taatataaaa acgaaaagag
    32101 gaatgaagat gagtcgaaag aaattaagtt taaaaacaaa aaaaataatc ataaatacta
    32161 taataattat tcttacaatt gcaataccat tagctatcac cgcatttgta gctgattatg
    32221 gttttaataa gagatataac ccaaattctg aatatgcaga taaaacaatt actgaccaaa
    32281 tcgcaagtga tttaagaagt gatgccttta ttgtgctaat ggaaggggct acgtttcctc
    32341 gcgagagaat tcaaaaatat gttggatata gaagtcaaaa atataattca actccaattt
    32401 atgaagaaac aatttataat gacaataatg aaaagctatt taatatgata gtttacgatg
    32461 gtttatggaa aaggattgaa gagggtgttg aatattatga catcgtttcg ccacaaatct
    32521 atatttacga tgttcaatat ggaaaaataa aagaagagtt tgaagttgac ccaactgtta
    32581 ttgaagatat tgaagatatc gaattaagtg taaagtttac tattccatat aaaagagata
    32641 gtggggttgc agacgaagaa agacaatatt cttttgctga cgtaaatggc gcaattactg
    32701 acacaggatc tgatcttaat acaaaacaag ttcaagcttg tgagttccgc gcagcattaa
    32761 gatttgcaga aacagaaaac attacagtta attttaacgc aaagattaca atcacttctt
    32821 caaatatagt agatcaagta ttcaagtcta ttaatttacc aattaatagc gatgcaagca
    32881 caagaaattt agacaatttt gaagagggtt taaatagttc ttttgatagt attattgcgc
    32941 ctcagttaaa agctgttgtg tttaaaaagt atatttggtg gcaatgctta atcgctttag
    33001 ttgtaagtag tgtcgttatc gttccttttg gtttatcgtt tatactacca ttaaaagaag
    33061 aaaaataagg agttggaaac aattccttat ttaataggag aaatagatat ggaattatac
    33121 gaaattgaga aagcattaat aaaaagatat cgaactaaat tatatgtacc nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcacaagatt taggatgcaa taaaatagcg
    33301 ctaggacatc atatgaatga tgttattgaa acaacaatgc ttaacatatt atatgctggt
    33361 tgttttaaaa caatgatgcc aaaattaaag tcaaaaaatt ttgaaggtat ggaagttata
    33421 agaccaatgt attatattaa ggaaaaagac atcattacat atatgaaata ctcagaaatt
    33481 gaaactatgc aatgtggttg cagaattgcg tctcgcgatt tagatagcaa aagagaagaa
    33541 gtaaaacaat taattaaaaa gcttaaaaaa acaaaccctg ttatcgaaaa acatattttt
    33601 aaaacagcat taaatgttaa tttagattgt gtgatagagt gggaatataa caataaaaaa
    33661 tataatttta tggattttta tagtgaaagc aaaaaaagcg atgatgacta aaatcattgc
    33721 ttttttagtt tctcaaagaa aattataaaa acgcttttat ttttcagtaa ttagtgttat
    33781 aatgttagaa agtatttgtt gaaggaggta acatatgtca aaacaacgat acacttatat
    33841 gtttaatgaa gtagatatga ctatgcgtaa cctttgtggt ggtaagggtg ctaatttagg
    33901 ccaaatgact aaattaggct tgcctattcc cgaaggattt atcgtaacta ctgaaacatg
    33961 ctcatacttt tatgagaacg gtaaaaaact atcgaaagaa attatcgaac aaatcaagga
    34021 atcactaaag attttagaaa aacaaacagg taaaaaattt ggtgatccaa acaatccatt
    34081 actactttca gtaagatcag gggctagagt ttcaatgcct ggtatgatgg atacaatttt
    34141 gaacttagga ctaaacgaca tcgttgctgc tggtttagcg cgcaaaacaa acaatcctcg
    34201 ttttgtttac gattcatatc gccgtttcgt tcaaatgtat gctgacgtag ttatgggggt
    34261 tggcaaaagt gaatttgaaa aaatcattga tgaaatgaaa gaagaaagag gtattaaatt
    34321 agatactgaa tttagcgctg atgatttcaa aaaaatgatt caaaagttca aaacaattta
    34381 caaaaaagat gttggcaaag atttcccaga aaatccagaa gaacaattgt ttggagctat
    34441 taatgcagta ttcttatcgt ggaatacacc aagagctatt tactatcgta gaatgaatga
    34501 tatcccacac gaatggggaa ctgcagtaaa cgttcaatca atggtatttg gtaatatggg
    34561 agatacatct ggaacaggag ttgccttctc acgtaatcct gctactggtg aagatcattg
    34621 ctacggagaa tatttaatga atgctcaagg tgaagacgtt gtagctggca ttagaactcc
    34681 tgaagattta gatcatttaa aagattatag tgaagaacta tacgatgaat taatcggatc
    34741 aatgaaaaaa ctagaagcat tctataaaga catgcaagat atcgaattca caatcgaaga
    34801 caataaacta tatctattac aaactagaag tggtaagaga acggctcaag ctgctattaa
    34861 aattgctata gatatgcaca acgaaggttt aattagtaaa gaagaagctt tattattatt
    34921 acaaccagca caattagatg acttattaca tcctcatttt gaagtagaag cattaaaagc
    34981 agcaaaacca ataggacaag gactaccagc atctccaggt gcagctacgg gcgaagtagc
    35041 atttacagtt gaaaaagcac ttgaagcaca caaagccggt cgtaaagttg ttttagtgag
    35101 attagaaaca tcacctgaag atatcgaagg tatggatatt tctgaaggat tcttaactgc
    35161 tagaggtgga atgacatcac atgcagccgt tgttgctcgt ggtatgggta aatgctgtgt
    35221 tgctggttgc catgaattaa gattccaaaa agattatgcg ttagttaatg atgtaaaaat
    35281 taaagaagga gatattatct ctttagatgg aactactggc cgcatttata aagaagcaat
    35341 tgcaacagaa ccagcaacta tttctggtga ttttgctaca gttatgaagt gggctaatga
    35401 agctaaacgt ttaggagtta gagctaatgc tgatacacca agagatgcaa aacaagcacg
    35461 cgaatttggt gctacaggta ttggattatg taggactgaa catatgttct ttggcgaaga
    35521 aagaattaaa gcaattagag aaatgattat tgctgattgt actaaagaaa gagaaaaagc
    35581 actagctaaa attttaccaa tgcaagaaca agactttgaa gaaatttttg aagcaatggg
    35641 cgactattca gttacaatta gactattaga cccaccacta cacgaattct taccaaaaga
    35701 agaaaaagat attgtagctt tagctaaaga cttgggtgta agtttagata gaatgaaatt
    35761 aagaatttca gaacttgaag aagcaaaccc aatgttgggt catagaggat gtcgtcttgc
    35821 aatctcatat ccagagttac caatcatgca aacaaaagct attattggtg cttattgtgc
    35881 tgtaaagaaa cgcaatccag catacaaagc agttccagaa attatgattc ctttaattgg
    35941 tgaaattaga gaattaagat atctaaaaga tgttattatt caaacagctg aagaagtttt
    36001 aaaagcaaac aaaatgaagc tagaatataa agtgggaaca atgattgaaa ttccacgtgc
    36061 agcattatta gctgatatta ttggagcgga agcagacttc tttagttttg gtactaacga
    36121 cttaacacaa atgacatttg gatttagccg tgatgatggt gcagatttct taaagactta
    36181 ttatgaaaag aaaatttatt taaatgaccc atttgctcgt ttagaccaag aaggtgttgg
    36241 taaattaatc gaaatgacag tacaaaaagg aagaaaagca aataagaaac ttaagattgg
    36301 tatttgtgga gagcacggag gagacccttc ttcagtagat ttcttccata gagcaggatt
    36361 agattatgtt tcttgttcac cttatcgtgt tccaattgct attttagctg ctgctcaagc
    36421 tgcaattaaa cacaaataac aaaataaaca agatataaag aaggagtaaa cttttactcc
    36481 tttttctttt tatgatataa tagcaaaaga ggtgatttta tgaaatatta taaataccac
    36541 gaagaaggaa atgattttct tattggcaaa gaaaaactca atttagatta taaaaaagaa
    36601 gctattaaat tatgtaatag aaaattagga gttggtgcta aaggattaat tgtttattct
    36661 aacaataata ttaatgtttt taattctaaa ggtgagaaaa aagatatcta ttttggcgga
    36721 attagggcta tttctacttg ttttaatgat caaggaattg actataaaaa tgaaataaat
    36781 tactcaggta acaaaataaa aattattgat gaagacttaa ttggctatga gcttaaaatt
    36841 gatcaaagta aactagcaga agatgagatt ctaacaacca aaggtaaaat tcctatttat
    36901 cgttatttag ataaaaagga acaagtctta ttaattacaa aaaatttaga tgagctagtt
    36961 aatagcggac tagctgaaga attatatgat aatccttatt tttctaaaga aaaagatatc
    37021 agttttgctc gcataataaa taaagacaat gtagttatct atacttataa cgaagatggc
    37081 tggttagagg gaaaagactt atcttgtgct tttgtttatt cgatacttga atataaagaa
    37141 atgatttcta aggaaataag ggttcattta aaaaaaggta ttttaaactt gaagaaagag
    37201 aatgacaaac tttatatttt tggtaaagca aataagatat gtaaaataga gatttaaata
    37261 aaaaaacaca ttagttttac taatgtgttt ttgttttata ttattttaac aagtttaata
    37321 ttcacaattt cctggtgttc ttgggaatgg taaaacatct cttatatttt caataccagt
    37381 aacatacatt actaatcttt caaatcccac accaaatccg gcatgtttag cagttccgta
    37441 ttttcttaag tttagatacc attgcatatc ttcaacagga acgttcattt ctttcatacg
    37501 tttttctagt aagtcataac gttcttctct ttggcttccg cccacaagtt ctccacaacc
    37561 aggaactagt aaatctacag ccgccactgt ttctccatct tcgtttaatc tcatatagaa
    37621 ggctttgata tctttaggcc aatcaattac aaatacaggg cctttaaaat gaacatcggt
    37681 taaatatttt tcatgttcag tagcaaggtc ttttccatat tcaggttttt cggtaaattt
    37741 atgacctgat tctttcataa ttttaattgc gtttttgtgt gtgcaaatag caatttttga
    37801 atttaaaaat gtttctaatt cttttatttt gccttttgca acaaattgat caaaaaaggc
    37861 catttcttct ggagcgtgtt ttaaaacata cttgattacg tattttaaaa gatcctcaat
    37921 taaagccata ttatcgttta aatctgcaaa gcatatttct ggttcaatca tccaaaattc
    37981 tgaagcgtgg cgttgtgtgt ttgagttttc tgctctaaat gtagggccaa aagtataaac
    38041 ttttttaaaa gcagttgcaa atgtttctgc ttctagttgg ccagacacag ttaggtgagc
    38101 cgcttttcta aagaaatctt gagaataatc tactttttta tcttttgttt ttggtaaatt
    38161 ttctaaatct aaagttgtta cttgaaacat atctcccgca ccttcagcat cactcgcagt
    38221 aataattgga gaatgtaagt aaacaaacct attttcataa aaatagttat gaatagcgta
    38281 agctaaaacg cttcttactc taaagacagc gttaaataaa ttagttctag gtcttaaata
    38341 agcttgttct cttaaaaact cgcgcgagtg tctttttggt tgaataggat aatcttctgg
    38401 cgaatcacct ataagttcta attttgtaac cttaagttca taaggttgtt ttgcattagg
    38461 tgttaaaaca actaatccag tagcagctat acttgcgcca actctaattt tcgaaacttc
    38521 tccaaagttt tctaatttat catcataaac aagttgtagt gttcctagag tagttccatc
    38581 agataaagca ataaatccaa actctttttg gtctctattt gatcttaccc aaccttgaac
    38641 cgtaatttct ttgttttcta gttgtttgtg tttttggtct aataaatata aatccatttt
    38701 tatttcctcc taaaatataa aaagtcctta aaaaaaacat tttaaggacg aaaaatttcc
    38761 gcggtaccac cttagttcgt ataaaatacg caatactcat tttacttttt aacgcaaagc
    38821 tacggttagg tctacttaat ttcttctaac agctcctagt gttctttcct ataaaatata
    38881 attacgactc tcaccattgt catatctctg taaatgattt ttatagtact tttctattca
    38941 tcgccttata gtttaatttt atcattatgc atcttttttg tcaagataaa actaagccct
    39001 attaacaagt tcaaaagtat aaaagttcct aaggaataag tccataaaag atatatccta
    39061 atacgcctga aataaaaatt aagagaacag gatgggcttt tttaaagatt tgggaaaggg
    39121 tgagaataac aaccattatt acaatagctt tccagtctag aagatagcca gtttcttctc
    39181 caataaacaa attcttagaa actaatgaca ctactacagc catcaacaac ccaattatta
    39241 ctggttttaa aattttaaaa acagacttta cataataatt ttctgaaaat ttagacatga
    39301 atttggcaat aatatatata ataagaaatg aaggtgtaac aacaccaagt gtagcaacca
    39361 ctcctcctaa tataccggct tgttggaagc caattaatgt tgcggtatta attgcaaatg
    39421 gaccaggagt tgactcagat attgctaaaa agtttaaaat ttcggttgtt gtaatc
//
LOCUS       DAOSGS010000003        22277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_1869_length_22277_cov_4.423904, whole genome shotgun sequence.
ACCESSION   DAOSGS010000003 DAOSGS010000000
VERSION     DAOSGS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 22277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22277
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_22277_cov_4.423904"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLQ22_00420"
     CDS             complement(<1..894)
                     /locus_tag="PLQ22_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH58457.1"
                     /translation="MKKFRILLLVFLGFILTGCSLFENPNHPFTNEIYNKSYNVETIT
                     IIDLENNIQAACEKASGAVLGITAHNLGAPIGTGSGVVYQGTIYLKNGTTKDIAAFLA
                     SDEVDHFEYKMITNRHVVTTEKGVKVSKVQVYDGDLDTNYNATILGTDNKVDLAVISF
                     SSPKYIRPLEFADSDLLKQGQFAIALGSPEGYEFYNSATFGIISHAKRYLADDTDNDG
                     TSDWDAEYIQHDVAINPGNSGGALINLEGKLVGINTLKLASTEIEGMGLAIPSNLVKK
                     LIPFLERGETPVRYVLGISVVT"
     gene            complement(881..1642)
                     /locus_tag="PLQ22_00425"
     CDS             complement(881..1642)
                     /locus_tag="PLQ22_00425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH58458.1"
                     /translation="MLVDTHIHLADNTYLEILDEIIEEAKGRFKSIIVSGVDYKTSLD
                     AIRLAERYDFLYASIGLHPQEVTKLENLDWIKKLAKHEKVIAIGEIGLDYYWDEQYKD
                     FQKELFIKQIKIANELKLPVIVHSRSAHQDTFDILKENKTKGILHCYSGSLELAREYT
                     KLGYYIGIGGVVTFKNAKTIKEVVSEIDLKYLLTETDGPYLTPHPYRGKINRPIYIEY
                     VIAEIARLKGKTKQEVEEKIYNNYLELFGGIDEEI"
     gene            complement(1632..2156)
                     /locus_tag="PLQ22_00430"
     CDS             complement(1632..2156)
                     /locus_tag="PLQ22_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58459.1"
                     /translation="MTKFKYELDYESQKRYINFHLFNHKRLHVSPVVLCIALVGFGIF
                     SIVLEQYLLTIVSAILIFVVLGFLFFLVRRVHKMNLKEKEFPEQNVSIAFTKTGMTLY
                     DDLSDDKNFIEYNKLHRIYFLKDYVYIYLTPDQGIALQRALLVEGDYDKFALFLKENF
                     SKKFVRFRGYKFVS"
     gene            complement(2302..3486)
                     /gene="tuf"
                     /locus_tag="PLQ22_00435"
     CDS             complement(2302..3486)
                     /gene="tuf"
                     /locus_tag="PLQ22_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOH58460.1"
                     /translation="MAKAKFVRTKPHVNVGTIGHVDHGKTTLTSAITKVLAKKGLSSA
                     MDYDKIDGAPEEKTRGITINSSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMAQTREHILLARQVGVPYIVVFLNKCDMVDDEELLDLVEMEVREALNE
                     YEFPGDDVPVIKGSALKAMEGDAKAEEAIIELMAAVDSYIPTPERDVDKPFLLPVEDV
                     FTITGRGTVATGRVERGTMRVGDEAEIVGIRPTLKTVVTGLEMFRKQLEFAQAGDNVG
                     ALLRGVSRDQIERGQVIAKPKTVNPHTEFAAEVYVLSKEEGGRHTPFFTNYRPQFFFH
                     TTDVTGVINLPAGVEMVMPGENANLNVSLIHPIAVEQGTKFTIREGGRTVAAGTVTKI
                     TK"
     gene            complement(3557..5629)
                     /gene="fusA"
                     /locus_tag="PLQ22_00440"
     CDS             complement(3557..5629)
                     /gene="fusA"
                     /locus_tag="PLQ22_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041639868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH58461.1"
                     /translation="MGRDVALHKLRNIGIMAHIDAGKTTLSERILYHTGKIHKVGETH
                     DGSATMDWMVQEQERGITITSAATTAYWNGYRYNLIDTPGHVDFTVEVSRSLRVLDGA
                     ITVLDAQAGVEPQTETVWRQATEYNVPRLVFANKMDKTGANFEYSISTIASRLGAKAC
                     AIQWPIGAEMFFSGIVDLVTMKAYHYEGDIDETCKEIEIPEDLILKVSEKREELIATV
                     ADYDEELMMTYLEGEEITPELLKQAIRKATLSVEFFPVLCGTAFKNKGIKLLLDAIID
                     YLPAPDDVPAIKAVRVSDNKELEILPADDAHFAALAFKVVTDPYVGKLTYIRVYSGVL
                     KTGTYVYNASREQKERIGRILLMHSNSRSEITEVYSGEIAAIVGLKNTTTGDTLCSEK
                     REIILENIIFPEPVISVAIEPKTKQDQDKMGIALMKLAEEDPTFKTYSNKETGQTIIA
                     GMGELHLDIIVDRLRREFNVDANVGAPQVSYRETITQPAEIQGKFVRQSGGRGQYGDV
                     HIRFEPNPGKGYEFVNAIVGGVVPKEYIPSVDKGLQTSMESGVLAGYPTIDLKATLYY
                     GSYHEVDSSQIAFEVAANYAFRETKNKCKPVILEPIMNVEVIVPDEYLGNVIGDLIGR
                     RGNIVSQEMRGNVQGLKAEVPLSSMFGYATSLRSNTQGRGNYTMQFSHYDRVPLAIQE
                     EIVAKRIA"
     gene            complement(5636..6106)
                     /gene="rpsG"
                     /locus_tag="PLQ22_00445"
     CDS             complement(5636..6106)
                     /gene="rpsG"
                     /locus_tag="PLQ22_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOH58462.1"
                     /translation="MPRKGHVPKRKVLPDPIYNSPVVTRLINNVMKDGKRGVAQEIIY
                     GAFARVEKQTGRPALDVFNDALTNITPELEVRARRIGGANYQVPCEVRPERKITLCLR
                     WLTQYSRLRREKTMEERLAAEIMDAANGQGASVKRRDDVHRMAEANKAFAHYQW"
     gene            complement(6133..6552)
                     /gene="rpsL"
                     /locus_tag="PLQ22_00450"
     CDS             complement(6133..6552)
                     /gene="rpsL"
                     /locus_tag="PLQ22_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH58463.1"
                     /translation="MPTVNQIIRKPRKAKEKKSKSVSLGINYNALKKKYTNVNSPQKR
                     GVCTRVGTLTPKKPNSAIRKYARVRLSNGIEVNAYIPGEGHRLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTLDTTGVEGRKQGRSKYGVKKPKDHK"
     gene            complement(6778..7521)
                     /locus_tag="PLQ22_00455"
     CDS             complement(6778..7521)
                     /locus_tag="PLQ22_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58464.1"
                     /translation="MNDKKQKPRIYLVGKEDKDKSIKVNFDINKVIKVLRKRNPLFED
                     FGDFFINFFYAINKLFPKSEHTLISSYDRYKRPGDEEIIRAVEFTVSVNNKMSVPIKL
                     EYKTKAANIKHHRVNYSLPNEGNHKFEAYNYLYGIFQMEKILKISHTKEGYTIFLTND
                     DTYLKKLDSILDADGFSLEDQSIKTGIINWTGDNSLLIEKEIPTLFTLKGEYKMEWKK
                     YSSYKYVDEKGNKNKGYFYVLITKVINEK"
     gene            complement(7537..8298)
                     /locus_tag="PLQ22_00460"
                     /pseudo
     CDS             complement(7537..8298)
                     /locus_tag="PLQ22_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            complement(8314..8775)
                     /locus_tag="PLQ22_00465"
     CDS             complement(8314..8775)
                     /locus_tag="PLQ22_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH58465.1"
                     /translation="MKFNYLINDDRVCITKYLGDEEEVVVPREIDGFPVVAIGDFAFR
                     GAKQIESIRIPDSVLSIGKEAFCDCENLQMISLPHSIVSIGANAFYKCKNLETIIIPD
                     NVTVLSYGLFQNCESLESVVLPDGLKIIGKKAFKDCQSLKHIILPEGITNI"
     gene            complement(8957..10072)
                     /locus_tag="PLQ22_00470"
     CDS             complement(8957..10072)
                     /locus_tag="PLQ22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOH58466.1"
                     /translation="MKLVAEYVSPGHPDRLCDQIVESIVTFVTTKDREALCSLECAVS
                     NKKVFIDGVIVAGKDALVITNSEIKDVVKKVYYDAGYNDSNETSYKWEPYLEDLEIIL
                     DVNIETLVDGERRIRKYSDDQNIVTGYAINSKETDYLPIAHFLANFIGRKIVEYRDAT
                     IFGPDFKVLVQLNYSGSVYTWDRLTVSIHHNRRKSFREMYAMMKSVIDKILEELNTLG
                     FNSLSKISNLNFYLNGAGDFVKGGPIGDSGLSGKKLCLDFYGPDIPIGGGAISGKDSF
                     KVDVCGAFRARELALELVKQHNYYSVFVTLAWSPGEAEPYLIQAFEIKKNGLRLRIDD
                     SLLPPKDWFSIECINYDLKLPEMKRREKVVQGYMFSK"
     gene            complement(10069..11391)
                     /locus_tag="PLQ22_00475"
     CDS             complement(10069..11391)
                     /locus_tag="PLQ22_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58467.1"
                     /translation="MNSEELIKKRIYSVMPVSSMHMMEFLKVLEIRFTDDVPTAAITS
                     RVRPKLLLNKAFIEKYCKTDEHLFMLIMHEIYHIILGHTRLFDNHTMLDNLAFDAVIN
                     AILCRLFPEEEYVSFFKNLNYDNVFPSCLLRPIGENTPVKFHKILNNLYDSYTGTYFE
                     VYEIICNTLGDKLLKDNFILLGNHSDNYASRDPLLKDMIDSITNNWQIGISGGRRIGS
                     SPIEKIIDFQSVKRDNQIKMQRLLNKAGMNLGVVPKMRISNELGKTEIEGFIPNFKDR
                     TVLSKTILYGRPLLYNREIDLIKPVSDPELKTLVYLDVSGSVVNSINSLASLLLKPYK
                     NGECLLFVFSTIVDEVTYKDFKEGRYRTTGGTDINCIFDHYFSLPKAKQTKKILILTD
                     GYTGRVNNRFYNEIKRKKLEIYCGLFGEYYTRDDLQPIIKYFEEFKYL"
     gene            complement(11388..11771)
                     /locus_tag="PLQ22_00480"
     CDS             complement(11388..11771)
                     /locus_tag="PLQ22_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58468.1"
                     /translation="MRRPRDSRVVFEKYQLGYYLIFHPETMAKILNTTVEELPLAVKE
                     YVDNNPYLHESKKKPKSYMLGEYYFMNKELKVEYEAEICEDLELLILVLCSTVRDIYI
                     PNRKLSFEKSKFNKVLFFPKEEVER"
     gene            complement(11768..13183)
                     /locus_tag="PLQ22_00485"
     CDS             complement(11768..13183)
                     /locus_tag="PLQ22_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOH58469.1"
                     /translation="MKQRKSLLTEPLGLFGWKTLEPVILAALATELPMLLVGKHGSAK
                     SFLLERLAESLNLNFRSYNASLINYDDLVGIPIPANNNTTLEYISNPNSIWDAEVVFI
                     DEINRTKPEFQNKLFPIIYDKRVQGQKLEKLKYRWAAMNPSFSDEEEEDNLIYFGAMP
                     LDPALSDRFPFIIEVPEWDSLSKEDRKKMLYDADKGRHEFPVDIKELVDKVKAKIKEI
                     SVSKQEEISNYILALMDVLKGSLGYVSARRATMFQETLIAIYASMLVLGEYTGDVPAL
                     DDSVYLHIQTSLPFIVNQRIERRLLLTVAARSLKIAQIGDAITRKLLLTTDPLKQIKF
                     AVVNRIAIEAETLSEVITSSISRLSTHKRRAMALISYLVFKENKDIYATVIETLAIEV
                     RPVFETRRVDIMEEARVKVLADHIDTLMSGVDENENYYKYLNNLLYSFLLPRTSQSFY
                     SNEEIDILFHFFIEIWKELVE"
     regulatory      complement(13238..13339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(13381..13920)
                     /locus_tag="PLQ22_00490"
     CDS             complement(13381..13920)
                     /locus_tag="PLQ22_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH58470.1"
                     /translation="MGLKYKETKEGLAITGYDGKIIDLVIPDAIDGIPVVAIDDRAFE
                     YCHSLKSIKFPNSITKIGNYAFFFCLGLEEIKLPRNLKSIGDYAFMNVSKVKYIVIPK
                     SVEDVGIDSFAFGSHDGRKKKIFLERKRVPKNWKSWHFDNVFIYLKNRWEYDENGIPV
                     VKWMNEEEAYSFLEENKRL"
     gene            complement(13953..17171)
                     /locus_tag="PLQ22_00495"
     CDS             complement(13953..17171)
                     /locus_tag="PLQ22_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH58471.1"
                     /translation="MKLTNEQIKAITVPQSKEERTNVLVSAGAGAGKTFVLIERIAKL
                     LKVCDLDELLVLTFTNQAAASMKEKLRTKLEEDESLRKYLEKIDNAKIMTFDAYSLFL
                     VKKYHLKLGLDPNIQIMDEYYKKTKLREIISEVLEEYYKENNETLKRYFKEDLIRNDE
                     TLINDLETLYYTLDKKVDTLDFIKNYKLNFYTKENLNDLKNRYIKLLNSFIDEEKTNL
                     QMLASYLDYKKEVLEEIGRDEGNLDDALIKWMDKVLPCINKTLSYEELKGVFSYQIPA
                     RSRKGNNIIQTELVNKAISEIREIIKDSKMQKQEFVSFEDEDDIIESIIYEKDYIFLF
                     LEMIEKIINKYSLYKAKYNVYDFSDIAKLAIKLVKENDDIRDEIKNSLYEILIDEYQD
                     TSDLQEDFINLISDYNVFMVGDIKQSIYLFRNANPFLFTEKLEKYSKGEGGQRVDLTS
                     NFRSRNEVLRDVDRIFKHLMTKDHGDVDYQDNHSLKAGNKNYETLGKLDKDSHMDLIG
                     YYSDKEKKKTDDEIEAFYIAQDILRRKGNDLVYDSKLGSLRPSTFKDYCILIDRSTEF
                     ESLKKILEFYSIPTIIYSDTVVEEEDIVYVLNALIRLVLYSSKDNLSKKEAQEYYHLF
                     YVLAKSFLFEYDDNYVAKIMIKVRDENKYYTKPYEYLDGKRSYFDPIFEKSYNLAKLV
                     NEASFTYIYDEILKEFNLYKHLPKIGNVKDQLTIIERIRSFIDFASLVGDDYFTISDK
                     ITGIKDGTDFKYTADQNEDVEAVKIMTIHKSKGLEFTYCYFPYLNKRFNFDDFTKIKI
                     GYLEESGLYCKRKTDKANVVFEVGYREAKRKQISEKIRLLYVALTRTKEKITIINKVN
                     PDKAPPAEFEFTDSLSSFGDFLNAIPNFKYDIQDVDVNKDYLSHSYKEYKYKLKKQNY
                     NDVSYLKEEVQEKRASKKVIKVLDEKEKQDIEYGNMLHAIMESVDFHNLESIESKEIL
                     NIIQDPKNIFFNDLNNSKFYQELEFIYQMDQEKVHGIIDLLIERDDIIYIVDYKASDI
                     DNKDYDKQLKVYYDYVRSRSSKNVECYLLSLIQNRFRKVDIK"
     gene            complement(17168..19480)
                     /locus_tag="PLQ22_00500"
     CDS             complement(17168..19480)
                     /locus_tag="PLQ22_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH58472.1"
                     /translation="MDLKEGIIICNLKEKQRILENLADFKNYKFYTLSEFLKNLTLTY
                     DKKALYYLNKELGISIDNLIEILNYLKYEQLFREISHEKTGLLKKIISILDNHKLIAR
                     NEQFRNIIKNKHITFLNVEDNFDFSFVCQILRSLGIEFESKKAEELTYKKRTVYAFVD
                     LVDEVRFVFNKIKELLDGGMPLDKIKIANYSSDYDFAFKRMSQLYKIPFNFESDKNIL
                     QTKTAKDVLKLFKENELKDAITILEKDYQDYPFYEEVISIINDYSLTSFDSAYAIKVF
                     TYAFENLRYPSPKYKDAIEFIDLKEHIISEDEVIFIIGFNNNFIPYVHKDVLYFDDNL
                     LNNLGMDNSEKRTQKEKELIKNKLNQNANYIISYKTNSVFNKYLKSPLIKELKYEEAK
                     LEPKIGYSKEEDYLLLTDVLDNYRKFKIKDAIFEENIRFKEEDFPYLDYDNQTEDLNE
                     ELVSQLIDFSKIYKFSFSSLDNYFKCPFKYYIENTLRLKYDDSPISSKIGTFIHEALA
                     LAYDSEYRIKDDLDYNRIFKDALANTEAKEKLEEKDKFIIGSLLKLTKDVIDYNLRAD
                     KGYKVICEPDDFVVRVTDKYQIGGKIDKIAYQEEDDHTNIVITDYKVRSIVKNPNFNN
                     LEYGLNLQLPIYAYLVAKSDKFTNPNVVGLYLQDVLKISELEKEPITMRGLDFSGNKL
                     LVENYKVGRMPTMDSSDLEVVLRALEKNFEKAYIGIKKGNIKITPKEIDGKNVSCEHC
                     SSRDICFKNYSNIEKLEAKDFRSLLIGDAE"
     gene            complement(19546..>22277)
                     /locus_tag="PLQ22_00505"
     CDS             complement(19546..>22277)
                     /locus_tag="PLQ22_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH58473.1"
                     /translation="LGKRVDYSGRSVIVIGPELKMYQCGLPREMALLLFKPFILHNLL
                     EKNKDGNDLTPNKIRATEAKEMIETGHPDAMTELEKIIVDHPVLLNRAPTLHRLSIQA
                     FEPKLIDGKAIRLHPLVTTAFNADFDGDQMSVHVPLSAEAQAEARILMLASKNILAPK
                     DGNPIVTPTQDMVLGNYYLTIEDPNDEREGKIFKDPYELELAYHNKVIGFHTRVALPV
                     ASLNNPRFTEDQLDKYIITTYGKYLFNTIFPVSDDPNKAISYICDQSEENLSRRTPNK
                     YLVPKGTDIKKFLKEKYESPRPFPKKALSKVIAQVFKNFQINETSKTLDKMKDLGFKY
                     ATMSGITVSISDIRTIEGKEEIIAAADEEVKEIKFKEDLGFATEEERRQNTIKVWTKA
                     KDDIKKLIEKDVALNSTTNNIYMMSDSGARGSIDNFVQLSGMRGLMAKPSGEQMDIPI
                     KASFIEGLSMSEFFVSTHGSRKGSTDTALKTAESGYLTRQLVDVSQEIVISEDDCKTD
                     KGKKVHPIFDTDDNEALGYKVYQAYLADPVNNPKPSSSNLQGVKLTLGQRIKGRFASQ
                     NVYDPVTGELYVKKNEFITEDIANAINASGLWEVEIRSLLTCNTQNGVCVKCYGSDLS
                     TSKVVERGEAVGVIAAQSIGEPGTQLTMRTFHTGGVAGGEDITQGLPRVNELFGAREP
                     KGKAIISEITGTIKIVDKQADNRTRIVVQNEIETKEYLTDVGKATKLQVGDKINAGDK
                     LVEGLIDPKELIRVSSVAAVENYILEEVQRVYQSQGVGIADKHIEFIVRQMLRKVKVT
                     DAGDTNLIVGSLISRAELQDIYQECILAGKKTPTVVPELLGIIRSALRADSFLSAASF
                     QETTKVLTEAAIRGKKDYLLGLKENVIIGGLMPAGTGLVDGDYYINENQEEAE"
BASE COUNT         7240 a         4110 c         2600 g         8327 t
ORIGIN      
        1 tgttacaaca ctaattccta aaacataacg aacaggtgtt tctcctcttt ctaagaaagg
       61 aattaacttt ttaactaaat tagatggaat cgccaatccc attccttcaa tttctgtact
      121 tgcaagtttt aatgtattaa taccaactaa cttaccttct aagttaatta aagcacctcc
      181 gctattacct gggttaattg caacatcatg ttggatatac tcagcatccc agtcagaagt
      241 accatcatta tctgtatcat cagctaagta acgcttagca tgagagataa tcccaaatgt
      301 agcactatta taaaactcat aaccttcagg actacctaaa gcaatcgcaa attgtccttg
      361 ttttaaaaga tcagaatcag caaattctaa tggtcttata tatttaggtg atgaaaaact
      421 aatcacagct aaatctactt tattatctgt acctaaaatt gtcgcattat agttagtatc
      481 taaatcacca tcataaactt gaactttgct aacttttact cccttttcag ttgtaacaac
      541 gtgtctatta gtaatcatct tatattcaaa atgatctact tcatcacttg ctaaaaaagc
      601 agcaatatct ttagttgtcc catttttaag atatatagta ccttgataaa caactccaga
      661 acctgtgcca attggtgctc ctaaattatg agctgtaata cctaaaacag caccacttgc
      721 tttctcacaa gctgcttgaa tattattttc taaatcaata attgtaattg tctctacatt
      781 ataacttttg ttataaattt cgtttgtgaa aggatgatta ggattttcaa ataaactaca
      841 tccagttaaa atgaatccta aaaagaccaa taataatatt ctaaatttct tcatcaatac
      901 ctccaaataa ttctaaataa ttattgtata ttttttcttc tacttcttgt tttgtttttc
      961 ctttaagcct tgcaatctca gcgattacat actcaatata tattggccta tttatctttc
     1021 ccctataagg atgaggtgtt aaatatggtc catcagtttc tgtaagtaaa tattttaaat
     1081 ctatttccga aacaacctct ttaattgtct tcgcattctt aaaagtaaca actccaccaa
     1141 taccaatata gtatcctagt ttagtatatt ctcttgctag ctctaaacta ccagaataac
     1201 aatgtaaaat accttttgtt ttattttcct ttaaaatatc aaaagtatct tggtgtgcac
     1261 tacgagaatg aacaattact ggtaacttaa gttcgttagc aattttaatt tgcttaataa
     1321 aaagttcttt ttgaaagtct ttatattgtt catcccaata atagtctaaa ccaatctcgc
     1381 caattgcaat tactttttca tgtttagcta acttcttaat ccaatctaaa ttctcaagtt
     1441 ttgttacttc ttgaggatga agaccaattg aagcatataa aaagtcatac ctttcagcta
     1501 atctaattgc atctaaacta gtcttataat caaccccaga aacaataata cttttaaatc
     1561 ttcctttagc ttcttcaata atctcatcta agatttctaa ataagtatta tcagctaaat
     1621 gaatatgtgt atcaactaac aaacttataa cctctaaatc ttacaaactt cttactgaag
     1681 ttttctttta aaaataaagc aaacttatca taatctccct ctacaagtaa tgctctttgt
     1741 aaagcaattc cttgatctgg agttaaataa atatacacat aatcttttag aaaatagatt
     1801 cgatgtaatt tattatattc aataaagttt ttatcatctg ataagtcatc atataaagtc
     1861 ataccagttt tagtaaaagc aattgaaaca ttttgctcag gaaattcttt ttcttttaga
     1921 ttcattttat gaactcttct aactaagaaa aacaaaaatc ctaaaacaac aaaaattaaa
     1981 atcgctgaca caatagttag taagtattgc tctaaaacaa ttgaaaatat cccaaatcca
     2041 actaaagcaa tacataaaac aactggactt acatgtaatc ttttgtgatt aaataagtga
     2101 aaattaatgt atcttttttg tgattcataa tcaagttcat atttaaattt tgtcatatca
     2161 tacacctcgt atctatttat tatagcataa aatgcttaaa attcaaaatt atttgtaatg
     2221 caaattatct aaaatttaaa gcttagaccc tataaaaaca aaataaggct taaagcctta
     2281 ttttgtttat cctaaattaa attatttagt aatcttagtt actgtaccag cagctacagt
     2341 tctaccacct tcacggatag taaacttagt accttgttct acagcaattg ggtgaattaa
     2401 tgaaacgttt aagtttgcat tttcaccagg cataaccatc tcaacgccag caggtaagtt
     2461 aataacgcct gtaacgtctg tagtatggaa gaagaattgt ggacgatagt tcgtaaagaa
     2521 cggtgtatgt ctaccacctt cttctttgct tagaacgtaa acttcagctg caaattcagt
     2581 gtgtgggtta actgttttag gttttgcaat tacttgtcct ctttcaattt gatcacgaga
     2641 aacacctctt aatagtgctc caacgttatc tccagcttga gcgaactcta attgctttct
     2701 aaacatttca agtccagtaa caactgtttt tagagtaggt ctaataccaa cgatttcagc
     2761 ttcatcgcca actctcattg ttcctctttc aactctacca gttgcaacag ttcctctacc
     2821 agtaatagta aatacgtcct ctacaggtag taagaatggt ttgtcaacat ctctttcagg
     2881 tgtagggatg tatgagtcta ctgcagccat taattcaata atagcttctt cagctttagc
     2941 atcgccttcc atagctttta atgctgatcc tttaattact ggaacatcgt ctccagggaa
     3001 ttcatactca ttaagagctt ctctaacttc catttcaact aagtctaata attcttcatc
     3061 atctaccata tcgcatttgt ttaagaaaac aacaatataa ggtactccaa cttgacgagc
     3121 aagtaagatg tgctctctag tttgagccat tgggccatca gtagcagcaa ctactaaaat
     3181 agctccgtcc atttgagctg caccagtaat catgttcttt acatagtctg cgtgacctgg
     3241 gcagtcaacg tgtgcatagt gtctgttttc tgtttcgtat tcgatatgag atgagttgat
     3301 agtaatacct cttgtttttt cttctggtgc accatcaatc ttatcataat ccatagcgct
     3361 tgataatccc ttcttcgcta acacctttgt gattgctgaa gtaagagtag tttttccgtg
     3421 gtctacgtga ccaattgtac caacgttaac gtgtggtttt gtacgtacaa atttagcttt
     3481 tgccattata attcctccta ttaattctct attattttat aacattttcg cataaatttc
     3541 aagtcattta tgccttttaa gctattcttt ttgcaactat ttcctcttgg atagcaagag
     3601 gaacacgatc gtaatgacta aattgcattg tatagttacc tcttccttgc gtattgctac
     3661 gtaaagatgt tgcataacca aacattgaag ataaaggtac ctcagcctta agaccttgaa
     3721 catttcctct catctcttgg ctaacaatat ttcctcttct tccaattaag tcaccgatga
     3781 catttcctaa atactcatct ggaactatta cttccacatt cataattggc tctaaaatta
     3841 ctggtttaca cttattctta gtttctctaa aagcatagtt tgcagctact tcaaaagcaa
     3901 tttgacttga gtctacttca tggtatgaac cataatataa agttgctttt aagtcaattg
     3961 taggatagcc tgctaaaact ccactttcca tagaagtttg taagccttta tctactgatg
     4021 gaatatattc cttcggtaca actccaccta caattgcatt tacaaactca taacctttac
     4081 ctggattagg ctcaaaacga atatgaacat ctccatattg tcctctacca ccagattgac
     4141 gaacaaactt tccttggatt tctgctggtt gtgtaatcgt ctcacgatat gatacttgag
     4201 gagcacctac attggcatca acgttaaatt ctcttcttaa tctatcaacg ataatgtcta
     4261 agtgtaactc acccatacca gctataattg tttgtccagt ttctttgttt gaataagttt
     4321 taaatgttgg gtcttcttct gctaacttca ttaatgcaat acccatttta tcttgatctt
     4381 gttttgtttt tggttcaatt gcaactgaga taactggttc agggaaaata atattttcta
     4441 aaataatttc tcttttttct gaacacaaag tgtctccagt agttgtattc tttaacccaa
     4501 caatcgccgc aatctcacct gaataaactt cagtaatctc actacgtgaa ttagaatgca
     4561 ttagtaagat tcttccaatt ctttcttttt gctctctaga tgcattataa acataagttc
     4621 ctgtttttaa aacacctgaa tatactctaa tgtaagttag tttccctaca taaggatcag
     4681 ttacaacttt aaacgctaaa gctgcaaagt gagcatcatc tgctggtaaa atttctaatt
     4741 ctttgttatc acttactctt acagctttaa tcgcaggtac atcatctgga gcaggtaagt
     4801 aatcaataat tgcatcaagt aataacttaa tacctttatt cttaaacgct gttccacaaa
     4861 gtacagggaa gaactcaact gataaagtag cttttctaat tgcttgtttt aataactcag
     4921 gagtaatttc ttctccttct aaataagtca tcattaattc ttcatcataa tcagctacag
     4981 ttgcaattaa ttcttctctt ttttcactaa cctttaagat taagtcttca ggaatttcaa
     5041 tttctttaca agtctcatca atatcacctt catagtgata agctttcatt gtaactaaat
     5101 ctacaattcc actaaagaac atttcagctc caattggcca ttgtatcgca caagctttag
     5161 ctcctaatct actagcaatt gtactaatgg aatattcaaa gttagcacct gttttatcca
     5221 ttttattcgc aaatactagt cttggaacat tatattcagt tgcttgtctc caaactgttt
     5281 ctgtttgcgg ttcaactcct gcttgagcat caagtacagt aatcgcacca tctagaactc
     5341 ttaaagatct tgatacctct accgtaaaat ctacgtgtcc tggagtatcg attaaattat
     5401 aacgataacc attccagtaa gctgtagtag cagctgatgt aatagtgata cctctttctt
     5461 gttcttgaac catccagtcc attgtagctg aaccatcatg agtttcacca actttatgta
     5521 tttttcctgt atgatagagt attctttcag ataatgtagt cttaccggca tctatatgag
     5581 ccataattcc aatatttctt aatttatgta atgctacgtc tcttcccata gtattctacc
     5641 attgatagtg agcaaaggct ttgtttgctt cagccattct atgtacatca tctcttctct
     5701 taactgatgc accttgaccg tttgcagcat ccataatctc cgcagctaat ctttcttcca
     5761 ttgtcttttc tcttcttaga cgagaatatt gagttagcca acgaagacaa agagtaattt
     5821 tacgctcagg tctaacctca caaggaactt ggtagttagc acctccaatt cttctagctc
     5881 taacctcaag ttctggagta atatttgtaa gtgcatcatt gaaaacatct aaagctggtc
     5941 ttccagtttg tttttcaact ctcgcaaatg caccataaat tatttcttga gcaactcctc
     6001 ttttaccatc tttcattaca ttattaatta atctagtaac aactggtgaa ttatatatcg
     6061 gatcaggtaa aacctttctc tttggtacat gtccttttct tggcatgtta ttcctccttt
     6121 cttattatta ttttatttgt gatctttagg ctttttaacg ccatatttac tacgtccttg
     6181 tttacgacct tcaacacctg tagtgtctaa tgtgcctcta acgatatggt aacgtacacc
     6241 tggtaagtct ttaactctac cacctctgat taatacaaca ctgtgttctt gtaatctatg
     6301 tccttcacct ggaatataag cgttcacttc aattccgttt gaaagtctaa ctctcgcata
     6361 cttacgaata gctgagttag gtttcttagg agttaaagtt ccaactctcg tacatactcc
     6421 tcttttttga ggtgaattaa cattagtgta ctttttcttt aacgcgttat agttaatacc
     6481 taaagaaact gatttactct tcttctcttt agcctttctt ggctttctaa tgatttgatt
     6541 tacagtaggc attctttctc ctttctgcca cattaccggg cgtttcattt tgtcatttta
     6601 tataggccct cttaaggcct actcaaaacc tctattattt taccaaatat ttcaataaat
     6661 gtcaagaatt aaatatgtat atttaaataa attttatgta aaaaggtcag ttgcctgacc
     6721 tttaatttat tttaatttca taaaaagctt aaaagtaatt ttatttaatc tcttttacta
     6781 tttttcattt atcactttag ttattaaaac ataaaaataa cctttatttt tatttccttt
     6841 ttcatctaca tatttataag aactgtattt cttccactcc attttatatt cgcctttaag
     6901 agtgaataat gttgggattt ctttttcaat tagtaaacta ttatccccag tccagtttat
     6961 tataccagtt ttaatacttt gatcttctaa agaaaaccca tcggcatcta aaattgaatc
     7021 aagtttttta agataagtat catcattggt aagaaaaata gtatatcctt ctttagtatg
     7081 agaaattttt aagatttttt ccatttggaa aataccataa agatagttat atgcttcaaa
     7141 tttatgatta ccttcgtttg gaagactata attaactctg tgatgtttta tgttggctgc
     7201 ttttgtttta tattcaagct taattggaac tgacattttg ttatttactg aaacagtaaa
     7261 ctcaactgct ctaattattt cttcgtctcc aggtctttta tatctatcat atgaagatat
     7321 aagagtatgt tcagattttg ggaataactt attaattgca taaaaaaagt taataaagaa
     7381 atccccaaag tcttcaaaaa ggggatttct ttttctcagt actttaataa ctttatttat
     7441 atcaaaatta actttaatag atttatcttt atcttctttt cctactaagt aaattcttgg
     7501 cttttgtttt ttatcgttca tttaatatcc tttcgtttaa tttggtatag gggttccgtc
     7561 ttgatcataa tgccattctc cagcccaata tacttttaca tttggggatt tccagccaaa
     7621 tacccatcct actagttcat ttggagtttc tgaaaatatt ggattttcat ttttcaattc
     7681 aaaagcatct ttctcaataa ctgttacatt agatgggata ataatatttt ttaatttgtt
     7741 gcaattgtaa aaagcagaat tgccaattct tgtgacactt tttggaataa cgaaatcttc
     7801 taaattagtg cacctatcaa aagtataatc tcctatttcg gtaaggcctt ctggaaagtt
     7861 tacttgtttt aaactataac accacataaa cgctgctctt tcaatctttc caatactttt
     7921 tggaatttga acaatttcta aacttgtaca taaatagaaa gccgaacgac caatgttttc
     7981 aacaccctcc tcaatgttga cttcttttaa cttagtacaa tttctaaatg cggcatatcc
     8041 tatgcttttg atatttttag ggatttttaa actagttaac atgtcacaaa aagcaaaagc
     8101 ccttttccca attgttttaa cttttttagg gatttttaac tcaagtaacg tcttacacca
     8161 ttcgaaagct ccttcttcaa tagtaattaa gcctttagga agctttagtt tgcgcaaact
     8221 aacacaaaag taaaagcact tcttactaat actctttaca ccttctggaa tatcaatcca
     8281 tggcacacta tagcatcccg aaaaagcttc ttcttaaatg tttgtaatcc cttcaggtaa
     8341 aatgatgtgc tttaagcttt gacaatcttt aaatgccttt ttacctataa tttttaggcc
     8401 atcaggaaga acaacactct ccaaactctc gcaattttga aaaagaccat aactcaaaac
     8461 tgtaacatta tctggaataa taattgtttc caaattctta catttataga aagcatttgc
     8521 tcctatacta acaatgctat gtggtaaaga tatcatttgt aaattctcac aatcgcaaaa
     8581 ggcttctttt ccaatactaa gaacactatc tggaattcta atgctttcaa tttgtttagc
     8641 ccctctaaaa gcaaaatccc ctatcgctac aacagggaaa ccatcaatct cacggggtac
     8701 aactacttct tcctcatcac ctaaatattt agtgatacaa actcgatcat cgttgattaa
     8761 ataattaaac ttcacattca ctctcctttc gaaatttatt tataatcaaa attgtgatta
     8821 agtatattaa cctccttatt gttttgcatt atagcaaatt ttaataataa agtaaataga
     8881 aatcgtaaaa caatattaaa taaaaataca aaaaaatgag tttatatttt ataaactcat
     8941 ttaatattta tcacttctat ttactaaaca tataaccctg tacaaccttc tcgcgtcttt
     9001 tcatttcagg aagtttaaga tcataattaa tacactcaat tgaaaaccag tctttaggag
     9061 gtaacaaaga atcatctatt cttaatctta aaccattttt cttgatttca aatgcttgaa
     9121 ttaaatatgg ttctgcttct cccggtgacc aagctagtgt cacaaataca ctataataat
     9181 tatgctgttt aactaattct aaagctaatt ctctagctct aaatgcacca caaacatcaa
     9241 ctttgaaaga atcctttccc gaaattgccc ctcctccaat tggaatgtct ggtccataaa
     9301 aatctaaaca taacttttta ccagataatc cactatctcc tattgggcct cccttcacaa
     9361 aatcacctgc tccatttaaa taaaaattta aattagaaat ttttgataaa gaattaaaac
     9421 ccaaagtgtt taactcttct aaaatcttgt caattacact cttcatcata gcatacattt
     9481 ctctaaagct tttccttcta ttatgatgaa tacttacagt tagtctatcc caagtatata
     9541 cactaccaga ataatttagt tgaactaaaa ctttaaaatc agggccaaaa attgtagcat
     9601 ctctatattc tactattttt ctgccaataa agtttgcaag aaaatgagct ataggcaagt
     9661 aatcagtttc cttagaatta attgcatagc cagttactat attttgatca tcactatact
     9721 ttcttatcct gcgttcacca tctactaaag tttcaatatt tacatctaat attatctcta
     9781 aatcctctaa gtatggctcc cacttataag atgtttcatt actatcatta taacctgcat
     9841 cataataaac cttctttact acatctttaa tttcactatt tgtgataact aaggcatctt
     9901 tgcctgctac tatcacacca tcaataaata ctttcttatt agaaacagca cattctaaac
     9961 tacaaagtgc ttctctatct ttagttgtta caaatgtaac aatcgactct acaatttgat
    10021 cacacagtct atcaggatgt cctggtgata catattctgc tactaatttc ataaatactt
    10081 aaactcctca aaatacttta taattggttg taaatcatct cttgtataat attcaccaaa
    10141 aagaccacaa tagatttcca atttcttcct tttaatttca ttataaaacc tattatttac
    10201 tctaccagta taaccatcag ttaagattaa tatctttttg gtttgcttag ctttaggtaa
    10261 agaaaaataa tgatcaaaaa tacagtttat atctgtacct ccagttgttc tatatcttcc
    10321 ctctttaaag tctttataag tgacttcatc tacaattgta gaaaagacaa ataataaaca
    10381 ctctccattc ttataaggtt ttaataataa tgatgctaaa ctattaatac tattaacaac
    10441 agaaccagaa acatctaaat aaactaaggt tttaagttca ggatcagaaa ctggtttgat
    10501 taaatctatt tccctattat aaagtaatgg cctaccatat aaaatagttt ttgatagcac
    10561 tgtcctatct ttaaaatttg gaataaatcc ttctatttct gttttcccaa gttcattaga
    10621 aattctcatt ttaggaacta ctcctaaatt catccctgct ttatttaata gtctttgcat
    10681 cttaatttga ttatcccttt tcacagattg aaaatcaatt attttctcaa tcggagaact
    10741 cccaattctt cttccaccag aaataccaat ttgccaatta ttagtaattg aatcaatcat
    10801 atcctttagt aagggatccc tgctagcata attatcacta tgatttccta gtaaaataaa
    10861 attatctttc aataacttat cacctaaagt attacaaata atctcataaa cttcaaaata
    10921 agtacctgta taagaatcat aaagattgtt taagatttta tgaaacttta caggtgtatt
    10981 ttcacctata ggccttaaaa gacacgaagg aaacacgttg tcataattaa gatttttaaa
    11041 aaatgaaaca tattcttctt ctggaaataa tcggcaaaga atcgcattaa taactgcatc
    11101 aaaagctaaa ttatctaaca ttgtatgatt atcaaaaagt ctagtgtgac ccaaaataat
    11161 atgataaatc tcatgcatta ttaacataaa caaatgttca tcagtcttac aatacttttc
    11221 aataaaagct ttatttaaaa gtagttttgg tctaacccta gaagttatgg cggctgttgg
    11281 cacatcatct gtaaacctaa tttccaaaac tttaagaaat tccatcatat gcatagagct
    11341 aacaggcatt actgaataaa tccttttttt aataagttct tcactattca tctttccacc
    11401 tcctctttcg gaaaaaacaa tactttatta aacttgcttt tttcaaagct aagcttacgg
    11461 ttagggatgt aaatatccct aaccgtggaa cacaaaacta aaatcaataa ctctaaatct
    11521 tcacaaattt ctgcttcata ttctacttta agttctttat tcataaaata gtattcacca
    11581 agcatataag atttcggttt cttcttactt tcatgaagat aagggttatt atctacatat
    11641 tcttttacag ctaaaggcaa ttcttctact gttgtattta atattttcgc catagtttct
    11701 gggtgaaaaa ttaaataata acctaattga tacttttcaa aaacaactct gctatctcga
    11761 ggtcttctca ttcaaccaac tctttccata tttcaataaa gaaatgaaac aaaatgtcta
    11821 tttcttcatt tgaataaaaa ctttgactag ttctaggtag taaaaatgaa tacaacaaat
    11881 tatttaaata tttataataa ttttcatttt catcaacacc actcattaaa gtatcaatat
    11941 gatcagctaa aactttaact ctcgcttctt ccattatatc tactcttctt gtttcaaaaa
    12001 caggtctaac ttcaattgct aaagtctcaa taacagtagc ataaatatct ttattttcct
    12061 taaaaactaa ataagatatt aaagccatag cccttctttt atgagtagaa agtcgactaa
    12121 ttgagcttgt aattacctca cttaacgttt cagcttcaat agcaattcta tttacaacag
    12181 caaatttaat ttgttttaaa ggatctgttg ttagtaacaa ttttcttgtt attgcatcgc
    12241 ctatttgcgc tatctttaat gacctagcag ctacagtaag tagtaacctt cgctctatcc
    12301 tttgatttac aataaaaggc aaactagttt gaatatgcaa atatacactg tcatcaagtg
    12361 caggaacatc cccagtatat tctcctaaaa caagcatcga agcataaata gcaattaatg
    12421 tttcttgaaa catcgttgct cttctagcag aaacatatcc taaactaccc tttaaaacat
    12481 ccattaaagc taaaatataa ttagatattt cttcttgctt gctaacactt atttccttaa
    12541 tcttagcttt taccttatca actaactcct taatatcaac aggaaattca tgtctacctt
    12601 tatcagcatc ataaagcatc tttttcctat cttctttact taaactatcc cattcaggaa
    12661 cttcgataat aaaaggaaac ctgtccgata aagctggatc taaaggcata gctccaaaat
    12721 aaattaagtt atcttcctct tcttcatctg aaaaagatgg attcatagca gcccagcgat
    12781 actttaactt ctcaagcttt tgtccttgaa ctcttttgtc ataaatgata ggaaataact
    12841 tgttttgaaa ttctggttta gtcctgttaa tctcatcaat aaatactact tccgcatccc
    12901 aaatcgaatt aggattagaa atatactcta aagtagtatt attgtttgca ggaatgggta
    12961 ttcctactaa atcatcataa ttaattaatg atgcattgta acttctaaaa tttaagttca
    13021 aactttcagc taatctttca agtaaaaaag atttagctga tccgtgttta ccaacaagta
    13081 acattggtaa ctctgttgct agtgccgcta aaattactgg ttctaaagtt ttccaaccaa
    13141 aaagtcctaa cggctctgta agtaatgatt ttctttgttt catcttttct ccttctacca
    13201 taaaggaaaa caaaaaaccc atcgctagat gggtttaatc acataaaaaa acaacccatc
    13261 attcaagctt tagcacctta ccttatggca ggttgctgaa gggtcataga gcttgtctct
    13321 caccttctct ttatggtact tatattatat cacataattt gtttttttgt actactttta
    13381 ttacaatctt ttattttctt ccaaaaaaga ataagcttct tcttcattca tccactttac
    13441 aactggaatc ccattttcat catattccca tctattcttt agataaataa aaacattatc
    13501 aaaatgccat gatttccagt ttttaggaac tctttttctt tctaagaata tttttttctt
    13561 cctaccatca tggctaccaa aggcaaaact atcaatccct acatcttcta cacttttagg
    13621 aattacaata tattttactt tagaaacatt cataaaagca tagtcaccaa tactttttaa
    13681 atttctaggt aatttaattt cttctaagcc caaacaaaag aaaaatgcat agttccctat
    13741 ttttgtaata ctattaggaa atttaatgct ttttaaactg tggcagtatt caaatgctcg
    13801 atcatcaata gctactacag gaataccatc tatagcatca ggtattacta aatcaattat
    13861 tttaccatca tatcctgtaa tagctaaacc ttctttagtt tctttatatt ttaatcccat
    13921 atgtttctcc ttgtttcaag taaataaatc ttttacttaa tatctacctt cctaaatcta
    13981 ttttgaatta aagaaagtaa ataacactca acattcttac tagatctact tcttacataa
    14041 tcataataaa cctttaattg tttatcatag tctttattat ctatatcact agctttataa
    14101 tctacaatat aaataatatc atctctttca attaaaaggt caataatacc atgaaccttt
    14161 tcttgatcca tttggtaaat gaattctagt tcttggtaaa attttgaatt atttaaatca
    14221 ttaaagaaaa tgttttttgg atcttgaata atatttaaga tttctttact ttcaatactt
    14281 tctaaattat gaaaatctac actttccata atagcatgaa gcatatttcc atactcaata
    14341 tcttgctttt ctttttcatc taataccttt ataacttttt tagatgctct tttttcttgg
    14401 acttcttcct ttaaataaga aacatcatta tagttttgtt ttttaagttt gtatttatat
    14461 tctttataag aatgagaaag ataatcctta ttaacatcta catcttgaat atcatattta
    14521 aagttaggaa tggcatttag aaaatctcca aaagaactta aactatcagt aaattcaaac
    14581 tctgccggtg gtgctttatc tggatttact ttattgataa tcgtaatctt ttcttttgtt
    14641 cttgttaggg caacataaag taatctaatc ttttccgaga tttgttttct ttttgcttcc
    14701 ctataaccaa cctcaaaaac aacattagct ttatcagttt tccttttaca atacaatcca
    14761 ctttcttcta aatagccaat ttttatttta gtaaaatcat caaagttaaa tcttttattt
    14821 aagtatggga aataacaata agtaaactct aatcctttag atttatgaat tgtcataatc
    14881 tttactgctt ctacatcttc attttgatct gctgtatatt taaagtctgt accatcttta
    14941 ataccggtaa tcttatctga aatagtaaaa tagtcatctc caactaaaga tgcaaaatca
    15001 ataaaagatc taattctttc tataattgtt aactgatctt tcacattccc tattttaggt
    15061 aaatgcttat ataaattaaa ttcttttaaa atctcatcat aaatgtaagt aaatgatgcc
    15121 tcattaacta gttttgctaa attgtaactt ttctcaaaaa taggatcaaa gtaagacctt
    15181 ttaccatcta aatattcata aggtttagta taatatttgt tttcatctct aaccttaatc
    15241 attattttag ctacataatt atcatcatat tcaaataaga aactcttagc taaaacataa
    15301 aacaaatgat aatactcttg agcttctttt ttacttaaat tatctttact tgaataaaga
    15361 actaatctaa ttaaagcatt caaaacataa acaatatctt cttcctctac aactgtatca
    15421 ctataaatga ttgttggaat agaatagaat tctaaaatct tttttaaact ttcaaattca
    15481 gtactacggt caattaaaat acagtagtct ttgaatgtgg aagggcgtaa agaacctagc
    15541 ttactatcat aaactaaatc attacctttt cttcttaaaa tatcttgagc aatatagaaa
    15601 gcttcaattt catcatctgt cttctttttc tctttatcag agtaatagcc aattaaatcc
    15661 atatgactat ctttatctaa ctttcctaaa gtctcatagt ttttattgcc agcttttaaa
    15721 ctgtggttat cttgatagtc tacatcacca tggtctttag tcattaagtg tttaaagatt
    15781 ctatctacat cacgcaggac ttcattccta cttctaaagt tgcttgttag gtcaactctt
    15841 tgaccacctt cacctttact gtatttctct aatttttcag taaaaaggaa tgggttagcg
    15901 ttacggaaca aataaattga ttgtttaata tcacctacca taaagacatt ataatcacta
    15961 attaaattga taaaatcttc ctgtaagtct gatgtatctt gatattcatc aattaaaatc
    16021 tcatataaag aattcttaat ttcatctcta atatcatcat tttctttcac aagtttaatt
    16081 gccagcttgg caatatcact aaaatcataa acattatact tagctttgta taatgaatac
    16141 ttattgataa tcttttctat catttctaaa aacaagaaaa tataatcttt ttcataaatg
    16201 atgctttcta tgatatcatc ttcatcttca aaagaaacaa attcttgctt ttgcatcttt
    16261 gaatctttaa taatttcacg aatttcacta atagctttat taactaattc tgtttgaata
    16321 atgttatttc cttttcttga tcttgctgga atttgataac tgaaaactcc tttaagttct
    16381 tcataactta aagtcttatt tatacaaggt aatactttat ccatccactt aattaaagca
    16441 tcatctaaat taccttcatc tcttccaatc tcttctaaaa cttctttttt ataatctaaa
    16501 tagctagcta gcatttgaag attagttttc tcttcatcaa taaaactatt aagaagttta
    16561 atataacgat tctttaaatc atttaaattt tcttttgtat aaaagtttaa cttatagttt
    16621 ttaataaaat ctaaagtatc aacttttttg tctagagtgt aatatagtgt ttctaaatca
    16681 ttaattaaag tttcatcatt ccttattaaa tcttctttaa aatatctttt taaggtctca
    16741 ttgttttcct tataatattc ttctaaaact tcacttataa tttctcttag tttagttttt
    16801 ttataatact catccataat ttgaatgtta ggatctaaac ctaactttaa atgatatttc
    16861 ttcactaaaa ataaactata agcatcaaat gtcattatct tagcattgtc tattttttca
    16921 agatatttcc ttaaagattc atcttcttct aattttgtac gaagtttttc tttcatactt
    16981 gcagcagctt gattagtaaa tgttaaaact aatagttcat ctaaatcaca tacttttaaa
    17041 agctttgcaa ttctttcaat caaaacaaaa gtcttaccag ctcccgctcc agctgatact
    17101 aaaacattag ttctttcctc tttgctttga ggaacagtaa tagctttaat ttgttcattt
    17161 gtcaatttca ttctgcatca cctataagta aacttctaaa atctttagct tctaactttt
    17221 caatattact ataattctta aaacaaatat ctctactaga acaatgctca caagaaacat
    17281 ttttaccgtc aatttcttta ggagtaatct taatattgcc tttttttata ccaatatatg
    17341 ctttttcaaa attcttctct aaagctctta aaacaacttc taaatctgaa gaatccattg
    17401 ttggcattct cccaacttta tagttttcaa ctagtagttt attaccagaa aaatctaatc
    17461 ctcgcattgt aataggttct ttttcaagtt cacttatttt caaaacatct tgtaaatata
    17521 aacctactac attaggatta gtaaatttat cactttttgc aactaaatat gcgtatattg
    17581 gtaactgtaa attaagccca tactcaagat tattaaaatt aggattttta actatagatc
    17641 ttactttata gtcagtaatg acaatgttag tatgatcatc ttcttcttga taggcaatct
    17701 tatcaatctt accacctatt tgatatttat ctgtaaccct aactacaaag tcatcaggct
    17761 cacaaataac cttataacct ttatctgctc ttaagttata atcaattaca tcttttgtta
    17821 gtttcaataa tgaacctata ataaatttat ctttttcttc aagtttctct ttagcttctg
    17881 tattggcaag agcatctttg aaaatacgat tgtaatctaa atcatctttt attctatatt
    17941 ctgaatcata agctaatgct aaagcttcat gaataaaggt accgatttta gaactaatag
    18001 gactatcatc atatttcagc cttaaagtat tttctatata atacttaaaa ggacatttaa
    18061 agtaattatc taaactagaa aaagaaaact tgtaaatctt actaaaatca atcaactgag
    18121 aaactaattc ttcatttaaa tcttcagttt gattatcata atctaaataa ggaaaatcct
    18181 cttctttaaa cctaatgttt tcttcaaata tagcgtcttt aattttgaac ttacgatagt
    18241 tatctaaaac atcagttaac aataaataat cttcttcttt actataacca atcttaggct
    18301 caagttttgc ttcttcatat tttaactcct taattaaagg tgattttaaa tacttattaa
    18361 aaacagaatt agtcttataa ctaataatat aattagcatt ttgatttaac ttatttttta
    18421 ttaactcttt ttctttttgt gttctttttt cgctattatc cattccaaga ttatttaata
    18481 aattatcatc aaaatataaa acatctttat gaacataagg aataaagttg ttattaaaac
    18541 caataataaa aattacttcg tcttctgata tgatatgttc ttttaagtct ataaactcaa
    18601 ttgcatcttt atattttgga ctaggatatc taagattttc aaaagcataa gtaaatactt
    18661 ttattgcata agcactatca aaactagtta gactatagtc attaataata cttattacct
    18721 cttcataaaa aggataatct tgataatctt tttctaaaat agtaattgca tctttaagtt
    18781 cattttcttt aaatagttta agtacatctt tagcagtttt agtttgtaag atgtttttat
    18841 cactttcaaa attaaaagga atcttatata actgactcat cctcttaaag gcaaaatcat
    18901 aatcactaga ataattagca atcttaattt tatctagtgg catgccacca tctaacaatt
    18961 cttttatctt attaaaaacg aatctaacct catcaactag atctacaaaa gcataaacag
    19021 ttctcttttt gtaagttaat tcttccgctt ttttagactc aaattcaata cctaaactcc
    19081 ttaaaatttg acatacaaag ctaaaatcaa agttatcttc tacattcaaa aaggtaatat
    19141 gcttgttttt tataatgttt ctgaactgtt catttctagc aataagctta tggttatcca
    19201 aaattgaaat aatcttcttt agaagaccag ttttttcatg actaatttct ctaaaaagtt
    19261 gctcatattt tagataattt aagatttcaa taagattatc aattgaaata ccaagctctt
    19321 tatttaagta ataaagggct tttttatcat aagtaagagt caagttttta agaaattcac
    19381 tcaaagtgta gaatttatag tttttaaaat cagctaagtt ttctaaaatt ctttgttttt
    19441 cttttagatt acaaatgata attccttctt ttaagtccat aattcacctc ttgttttatt
    19501 ataatatatt aagttgaata tatcaagatt aaaaaaagaa gcagattact ctgcttcttc
    19561 ttgattttca tttatataat aatcaccatc tactaatcct gtacctgctg gcattaatcc
    19621 accaataatt acattttcct ttaagcctaa taagtagtct tttttacctc taatagcagc
    19681 ttctgtaagt acttttgtag tttcttggaa tgatgcagct gacaagaatg aatcagctct
    19741 taatgctgaa cgaatgattc ctaaaagttc aggaacaact gtaggtgtct ttttacctgc
    19801 tagaatacat tcttgataga tgtcttgtaa ttcagcacgt gaaattaaac taccaacaat
    19861 taaattagta tcacctgcgt cagtaacctt aactttacgt agcatttgac gaacaataaa
    19921 ttcaatgtgt ttatcagcga ttcctacacc ttgagattgg taaactcttt gtacttcttc
    19981 taagatatag ttttctaccg ctgcaactga tgaaactcta attaactctt ttggatcaat
    20041 taatccttct acaagcttat cgcctgcgtt aattttatca cctacttgaa gtttagttgc
    20101 cttacctacg tcagttaagt attctttggt ttcaatttca ttttgaacga caattcttgt
    20161 tctattatca gcttgtttat ctacaatctt aattgtacca gtaatttctg agataattgc
    20221 tttaccttta ggttcacgag caccaaaaag ttcgttaaca cgtggaagac cttgagtaat
    20281 atcttcacca cctgcaacac caccagtatg gaaagtacgc attgtaagct gtgtacctgg
    20341 ctcaccaatt gattgagcag caattacacc tacagcttca ccacgttcta caactttact
    20401 tgtagaaagg tctgagccgt aacatttaac gcatacacca ttttgagtat tacatgtaag
    20461 taatgatcta atttcaactt cccaaagacc tgatgcatta attgcattag caatatcttc
    20521 agtaatgaat tcatttttct ttacatataa ctcgccagta actggatcat aaacattttg
    20581 ggaagcaaaa cggcctttaa ttctttgacc taaagtaagt tttacacctt gtaagttact
    20641 tgaacttggt tttggattat taactggatc agctaaatat gcttgatata ctttataacc
    20701 taaagcttca ttatcatcag tatcaaagat aggatgaact ttcttgcctt tatctgtctt
    20761 acaatcatct tctgagatta caatttcttg gcttacgtca actaattgtc tagttaagta
    20821 tccagactca gctgtcttta aggctgtgtc tgtcgatcct tttcttgaac cgtgtgttga
    20881 aacgaagaac tcagacatcg ataaaccttc aataaatgat gctttaattg gaatatccat
    20941 ttgttcacca ctaggtttag ccattagacc tctcatacct gataactgaa caaagttatc
    21001 aatactacct ctggcaccag agtcactcat catatagatg ttgtttgtag ttgagtttaa
    21061 agctacatct ttttcaatta acttcttaat atcatcttta gctttagtcc aaactttaat
    21121 tgtattttgt cttctttctt cttcagttgc aaaacctaag tcttctttaa atttaatttc
    21181 tttaacttct tcatctgcag cagcaataat ttcttcttta ccttcaattg ttctaatatc
    21241 actaattgat acagtaattc cagacattgt agcatactta aaacctaagt ctttcatctt
    21301 atctagagtc ttagatgttt cgttaatttg gaaattcttg aaaacttgag cgataacttt
    21361 tgataaagct ttcttaggga atggtctagg tgattcatac ttttctttca agaacttctt
    21421 aatatcagta cccttaggaa ctaaatattt attaggagtt cttctactta agttttcttc
    21481 actttgatca cagatataag aaatagcttt atttggatca tcacttactg ggaaaatcgt
    21541 attaaataga tattttccat aagtagtaat aatatattta tctaattgat cctctgtaaa
    21601 acgaggattg tttaaagaag caacaggtaa ggcaactctt gtatggaaac caataacctt
    21661 gttatgatat gcaagctcta actcataagg atctttaaag attttacctt cacgttcatc
    21721 atttggatct tcaattgtta agtaataatt tcctaatacc atgtcttgag ttggcgtaac
    21781 aattgggttt ccatctttag gagctaggat atttttactt gcaagcatca agattcttgc
    21841 ctctgcttga gcctcagctg atagcggaac atggactgac atttggtcac catcaaagtc
    21901 tgcgttaaaa gctgtagtta ctaaaggatg taacctaata gctttaccat caattagttt
    21961 aggttcaaat gcttgaatac ttaaacggtg cagtgttggt gcacggttta ataacactgg
    22021 atgatctaca ataatctttt ctagttcagt cattgcatct ggatgaccag tttcaatcat
    22081 ctcttttgct tctgttgctc taattttatt aggagttaaa tcattaccat ctttattttt
    22141 ctctaaaagg ttgtgtaaaa taaatggttt gaaaagtaaa agtgccatct cacgtggtaa
    22201 tccacattga tacatcttaa gttcaggccc aattacgata actgaacgac ctgaatagtc
    22261 tactctctta ccaagta
//
LOCUS       DAOSGS010000004        17508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_2645_length_17508_cov_5.313642, whole genome shotgun sequence.
ACCESSION   DAOSGS010000004 DAOSGS010000000
VERSION     DAOSGS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2645_length_17508_cov_5.313642"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLQ22_00510"
     CDS             <1..795
                     /locus_tag="PLQ22_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HOH58474.1"
                     /translation="KSEAYWRAFGRYVQSLNGRYITAEDVNTNTDDMDYIAMETSNIT
                     GLKKTSGDPSPFTASGVYWGMRASCLEMYGSDELKDKKVLVQGIGQVGYNLVKFLVQD
                     GAKVFVTDIKKAKLDRIASDFPVTIIPPEDIFKDNYDIFAPCALGAIINDETIPQFKF
                     KIICGAANNVLKEERHGIALQNLGILYAPDYVVNAGGVINVYQEILGYDSEEAKRKVK
                     KIYNKVLEIFKIAKEKEITPAQAANHLAEERIRVIRNIRSNYIVRK"
     gene            792..1457
                     /locus_tag="PLQ22_00515"
     CDS             792..1457
                     /locus_tag="PLQ22_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH58475.1"
                     /translation="MKAPLIIIVGYFGSGKSEFAVNLALKLAKEQSVCLADLDIINTY
                     FRSRETREILMQQQIEVLSDTYNSTKGLDMPYLSPAIKGRIVKRDKLVILDCGGDTNG
                     IKVLKQYYDDIVDKPYEMWMVINVFRPETRNAEQILKMMQALKNESGLEVTGFINNSN
                     YLKSTTIEDMKTSNQIMEEVVALTKIKVVYTSGVESLMQQLPIDILGEKFPLKILLRE
                     KWL"
     gene            1470..1688
                     /locus_tag="PLQ22_00520"
     CDS             1470..1688
                     /locus_tag="PLQ22_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH58476.1"
                     /translation="MAKGKIEVAIERCKGCGLCIAACPLDLLELDPHSLNTKGYQPMR
                     IHTPEKCIGCANCATMCPDVVITVYRLD"
     gene            1750..2817
                     /locus_tag="PLQ22_00525"
     CDS             1750..2817
                     /locus_tag="PLQ22_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOH58477.1"
                     /translation="MAKLMMKGNEAMALAALKGGCKAFFGYPITPQSELPEYLSRYMK
                     EYGGVFLQAESEIAAINMVYGASAAGVRVMTSSSSPGIALKQEGISYMAGSELPGVII
                     NVMRGGPGLGGIQPSQSDYKQVTRGGGNGDYYCLAFAPESLQETVDIIKEAFDLSDLY
                     RNPVMIAVDGLIGQMMEPVDVDKEIKKHKISEKDWATDGTMGQRERRIVNSLYLNTPE
                     LEEHCIKLHTKYQKMKENEQRYEIVHGDDAEIMIVAYGTMARICRSAIEILREQGILV
                     GLIRPISIWPYPEIAFDQLSPTVKGLLTCELSMGQMIDDVKIANKGRLPISFFGRSGG
                     MIPETEEIVEAVMKMRGGLVK"
     gene            2814..3569
                     /locus_tag="PLQ22_00530"
     CDS             2814..3569
                     /locus_tag="PLQ22_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH58478.1"
                     /translation="MSKIVFERTKALTDNEFSYCAGCTHGIIHRLVAEVLEELNLIDQ
                     AIGISPVGCAVTNYNNFNCDMVQAAHGRGPAVATGIKRVIPNRIVFTYQGDGDLASIG
                     TAEIVHAAHRGEKITVIFVNNAIYGMTGGQMAPTTLIGQKTSTSPFGRTIEQSGMPIR
                     MSELLATIPGAAYIERVSVHNPAHVRKAKQAVKRAFQTQFDQKGFSLIEFISTCPVNW
                     GMTPNKALKWAEENMIPYYPLGVFRDDTKEVKG"
     gene            3569..4117
                     /locus_tag="PLQ22_00535"
     CDS             3569..4117
                     /locus_tag="PLQ22_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH58479.1"
                     /translation="MSEKIICAGFGGQGVMLIGQLLAYAANERGFNTLWYPSYGPETR
                     GGTANCSVTISENPVNSPVISKADTIIVMNKPSLEKFENKIAPLGKLFINSSLIDQKT
                     SRTDIEAYYIPANEIAGKLGNFKVANMIILGAYLEKTHLFDDQLIIKLLAKLFGESKA
                     HLIALNNEALVAGRNYFNKNYR"
     gene            4297..6339
                     /locus_tag="PLQ22_00540"
     CDS             4297..6339
                     /locus_tag="PLQ22_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58480.1"
                     /translation="MRFIRKNLKAFIFLLSLVAFVFIAMAIANGIQKDFGNIDVSVGT
                     LETTFKTMDGEEKEGEIAYKLYIPKTATSTNQASALLLLHGYQNDHETSAAYALELAR
                     RGVVVLAIDEFGHGATTISLVNRGYVNHKVTVNYGTESEDNQTFVEIGGPTRYKIMMN
                     FSNMKFFLDKYSKDDDGNQLLDSSMGGIAAYAYLSSLPYVNNKKMAVSGHSMGTWASW
                     SVSAAYSGNEIAPKATILQAGELFTKEAYDSTNIHFNNVLLLTAKWDEFNMFRDYSKQ
                     TVNDSIIQNEITSEFLGIVLGTGKWNTTYGDFENGSARRRELILTNHRLLTHNKKAIG
                     TTIDWLDETIGINTTLDNANQVFAIKEVLVLVATFSAIVSMFALFMILIKIPFFTSIA
                     HPEIILERAGSVKTGWKWWKGAIITILIAGLTYPFMTQLGHGLLSLPEMTVFRMTIGN
                     GFFSWYLILIIIMLLTTILPWRKAKKLNIPKDYCDLGLSTPENKDRFDWVLLGKSAIV
                     VACMILFMYLQCIICEKAFRLDFRFIWPFFKGFSGERFIQLLVYLPIFILFFVLNNSK
                     IFAQMRNSGTNKPGFKGFLSCWWRNALLMIGGILLLILVEYIPFFMGIGPGMDLLFSS
                     TFGGPFMSLMIVFVPQVLVFSLLCTFAYRKTGSVFVGALTVAVLACWIITGGSAIL"
     gene            6525..7178
                     /locus_tag="PLQ22_00545"
     CDS             6525..7178
                     /locus_tag="PLQ22_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOH58481.1"
                     /translation="MDKLFPSEIDQIVTGISYTIDDIGCSGDKVFLFEKKYVLKISNN
                     KERLKNEKMKIDWIASYIPSSKSVAFYEDEMNGYYLRTYLDGDSLIHKRFLSHPIKLI
                     EILRQAVLLLRGLDDKECPFQSIDNKGDAFIHGDLCLPNILVDENDDIIGFIDLDNSG
                     KGDPWYDYSWLLWSLEFNLKTDRYNHKLLQALGISFRKEKYEQYIPFEYISDLNRNK"
     gene            7354..9426
                     /locus_tag="PLQ22_00550"
     CDS             7354..9426
                     /locus_tag="PLQ22_00550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOH58482.1"
                     /translation="MEKYFILSTIVVSIVAFVFAFWLYLWVSKQPSSNKKVASIGKLI
                     RKGANTFLRKEYIVLAKFAGVIAILIFVFLPEPIWKGNILANLTMLISYVFGTGLSAL
                     AGKIGIQVATIANVKTAEAAQKGIKPSFLNGFRGGAVMGMAVVGASLLGIALVFLITE
                     NSTALLGFSFGASSLALFAKAGGGIFTKTADISADLVGKVELGIPEDDPRNPAVIADN
                     VGDNVGDVAGMGADLFDSNVASLAAALVMAVALGGVTGKNVMMVFCFATIGLLASVIG
                     VGAAKMGKKGNPTHALNMSTYVTTAIFIALTFVATLIFKFEWRIWGASVVGLLVGVII
                     GITTDYFTNDERKPVRNVAKASESGPAFTIINGVSYGFLSVLPAMVGIAISALVAYKL
                     CEPLGANYGMFGISMAAVGMLSIVGMIISNDAYGPIVDNARGLVEMGNLGETALEITD
                     SLDSAGNTVKAVTKGFSIGAAGLTVIALLGAFMSEVNVKALELGYDLITGFDIMDPVI
                     FFGLLVGAAVPAVFSALLMLGVNRNAERMVKEIHRQFQEIPGLVEGKEGVIPEYDKCI
                     SIATTGALKELIPAGLFAIVATIVVGFVGGVKAVGGFLVGNIVSGLLLALFMSNSGGL
                     WDNGKKYVESGNCGGKGSFAHKAAVVGDTVGDPFKDTAGPSINTQITVVSLLASLLST
                     IFLALSIF"
     gene            9763..11610
                     /locus_tag="PLQ22_00555"
     CDS             9763..11610
                     /locus_tag="PLQ22_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOH58483.1"
                     /translation="MKFWKKIKYKFDQLLSKGTISLVLALFTVTFIAVVLIGLIAYWS
                     TKSTDSALSMIWTSFMKTLDPGNLEGEEGTFFYVLLMTIATIVGIFITSILISIISSG
                     FQTKLENLQRGTSQIIEKNHTLILGWNDNVPIIVSQLIEANKSVKHPKIAILSEQDMM
                     ETYHALKQLVKNFHNTKIIIRTGSLFDKNSLETCAIDCARSVIISAIDDVKIVKTLLA
                     IKQTSFFNDLKSGYITAIFTNPKTMKLAQEMCGDKLVALHLTDSTNRIMAQTCLQPGL
                     SLVYQELFDFNGNEIYFYKNNQLVGKTVHDAIMKFNDSILIGIQRNQETLINPPKDMI
                     ISEEDEAILIAEDEDKIFINEDNYSLYEDKIILKPHNKTKSIRNILSIGFNNDTCKVI
                     KELLPYIEKGTHVKFLIPDNLDEQLFEEIEDQDLIEHDIIRGVTYERETLEAINYTNI
                     DTIIVFANSQDDGERSDSETLLTVLNLKQIEKERKFNASIIIEIEKNENEYILKYASV
                     DDFIISNVLANKMLCQISENRNLRKVFQELLGEEGSEIYLKHVEDYVTINEEVNFYTI
                     SKSASLKNEIAIGYKKKAVSENGGIVINPKKDKQVVFSKGDSIILIAID"
     gene            11644..12066
                     /locus_tag="PLQ22_00560"
     CDS             11644..12066
                     /locus_tag="PLQ22_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH58484.1"
                     /translation="MESEKRKEVIRIIKFTIFSISAGIIQLVSFTILSELFDITYWPS
                     YLTALILSVLWNFTINRKFTFKSANNVPIAMTKVLVYYAIFTPLSTWWGDALTKANWN
                     EYLVLGGTMIINFVTEFLYQRFFVFGKSIESAVPNKEA"
     gene            12170..12382
                     /locus_tag="PLQ22_00565"
     CDS             12170..12382
                     /locus_tag="PLQ22_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH58485.1"
                     /translation="MKNHLEKLRQERGIKQEDLAKAMGVSRQTIGSLENGRYNPSILL
                     AYRIAYFFNLKIEDIFIYEEKNNEDI"
     gene            12369..12776
                     /locus_tag="PLQ22_00570"
     CDS             12369..12776
                     /locus_tag="PLQ22_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HOH58486.1"
                     /translation="MKTFKKTYFEILFIIGLLLSALVSFDRGYHFLPDFIGGMLTSLS
                     IVLIVFAGIVKHSKKLQDKLQIADRDERLLTIEQKAAKITLYAIMFINIVCIIIFGFL
                     GDTYLYMSILLAIILFITIVILFITKLVLTKRM"
     gene            12791..13495
                     /locus_tag="PLQ22_00575"
     CDS             12791..13495
                     /locus_tag="PLQ22_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HOH58487.1"
                     /translation="MGKIYVFGGGEISQKETYLLDKLVVSKLNKQHPKVLFIPTASHD
                     AEGYVASFTRIYESLGCIVDSLLLVKENITDDVIKEKILNADLIYVGGGNTLFMMDTW
                     RRYKVDELLIKAYHHGVVLSGLSAGGICWFDYGYSDSLIIEGGYEYTYVEGLHLIHMD
                     HNPHAEEEERQGFLNSFKKRKKKALSMDNLTLAYFENGYLKTGYKCNEKAKAILVQSS
                     GEEVTCEEVFLEFILE"
     gene            13595..13816
                     /locus_tag="PLQ22_00580"
     CDS             13595..13816
                     /locus_tag="PLQ22_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58488.1"
                     /translation="MKMSHHFPLDKLKVEEDVNAIPSITDEDHKYYLSLYQDEQLVAV
                     IDFIDGFSFKKQNNENTIWIGLLQINKTN"
     gene            14107..14682
                     /locus_tag="PLQ22_00585"
     CDS             14107..14682
                     /locus_tag="PLQ22_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH58489.1"
                     /translation="MDSYVTSTMIKKLREEKRLTQKELADIILVSEKTISKWETGKGL
                     PDISLLEPLSKALGVSLIELFQGEAIKNKNKAANLLKTRFYVCPVCGNVVTSIGEGSF
                     SCCGETLLVQEVEKVDDIQIERIDNELYIMVVSPMSKIDYISFIAYCTTDRIQIKKLY
                     PEQNAEALFAINGPGLIYYYNNRNGLFVKKV"
     gene            14869..15141
                     /locus_tag="PLQ22_00590"
     CDS             14869..15141
                     /locus_tag="PLQ22_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH58490.1"
                     /translation="MDNQDFLNKLKIINDPTRLKIIQLLAKNKTMCACKILEELNITQ
                     GTLSHHMKSLVLAGFVGCLRKGKWCHYTLLPMNFKEIISVLNKFNE"
     gene            15387..16367
                     /locus_tag="PLQ22_00595"
     CDS             15387..16367
                     /locus_tag="PLQ22_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOH58491.1"
                     /translation="MKWLNIGVGKLLILIVGDFASGTWQNTLIEAIHFFIYDFIKIGI
                     LLCLLIFTISYVQSYFPPERTKRILGKYKGIWANFIGALLGTVTPFCSCSSIPIFIGF
                     TRAGLSSGITFSFLISSPLVDLGSLMLLVGFFGWNVAIIYVVVGLLLAVVGGTIIEKT
                     GLGKYVEDFVTSGSSIINVDLPELTIKDRLEYARDQMLQIYKKVFWYIVIGVGIGSII
                     HNVIPREWIEGLLGANKWYSVFLATLLGAPMYADIFGTIPIAEALFIKGAGLGTILAF
                     MMSVTALSLPSMIMLRKAVKPKLLFAFISIVITGIIIIGILFNLYGNVFI"
     gene            16402..16647
                     /locus_tag="PLQ22_00600"
     CDS             16402..16647
                     /locus_tag="PLQ22_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOH58492.1"
                     /translation="MKIQALGGCCKKSQENYKSIVEAVKLLELDVVVEHVTDMNAIFE
                     LGVMATPGLIVNGKILVTGRMLNVSQAKELISKELKL"
     gene            16644..17048
                     /locus_tag="PLQ22_00605"
     CDS             16644..17048
                     /locus_tag="PLQ22_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOH58493.1"
                     /translation="MKKNIAFVCVHNSCRSQIAEAFGKLYLSKITNVYSAGTILANQI
                     NQDAIRLMRKLYDIDLEKTQKPKLISSLPKIDYLITMGCNVECPLIPCKVHLDWGIED
                     PTYFLEEKFIKTIKMIEKKVLSLKVEIKKHFN"
     gene            17104..>17508
                     /locus_tag="PLQ22_00610"
     CDS             17104..>17508
                     /locus_tag="PLQ22_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOH58494.1"
                     /translation="MLKENVTKLLNEQINKEMYSAYLYFDMANFYVNKGMNGFANWFN
                     VQAKEEMDHALLFVKYLQNNGFEVSLAAIAKPDKVFKDLREPLAEALKHEEYVTDSIN
                     KIYTEALKVADYRTQEFLNWFIKEQGEEEKNAN"
BASE COUNT         6204 a         2215 c         3329 g         5760 t
ORIGIN      
        1 aaatcagaag cttattggcg tgcatttggt cgttatgttc aatcgttaaa tggtcgatat
       61 attacagcag aagatgttaa taccaataca gatgatatgg attacatcgc gatggaaaca
      121 agtaatatca ctggtttaaa gaaaacttct ggtgacccat ccccatttac tgcatcaggt
      181 gtctactggg gaatgagagc ttcttgccta gaaatgtatg gttcagatga attaaaagat
      241 aaaaaagttc ttgttcaagg aattggtcaa gtaggttata atctcgtgaa gtttctagta
      301 caagatggcg ctaaggtatt tgttactgat atcaagaaag caaaacttga tcgtatagct
      361 agtgattttc ctgttacaat tattccaccc gaagatattt ttaaagataa ctatgatatt
      421 tttgctccat gtgcattagg agccattatt aatgatgaaa caattcctca atttaagttt
      481 aagattattt gtggcgcagc gaataatgtt ctaaaagaag aacgtcatgg tatagcatta
      541 caaaatttag gaatcttata tgcacctgat tatgttgtaa atgctggtgg agtcatcaat
      601 gtttatcaag aaatcttagg atatgattct gaagaagcaa aaagaaaagt gaaaaaaatc
      661 tataataaag tattagaaat atttaagatt gcaaaagaaa aagaaattac gcctgctcaa
      721 gcagccaatc atcttgcaga agagcgaatt cgagtaattc gtaatattcg aagcaattat
      781 atagtacgta aatgaaagcc cctttaatta ttatcgttgg ttattttggt agtggaaaat
      841 cagaatttgc tgtcaatctt gcactaaaac tagccaaaga acaatcagtt tgtttggcag
      901 atttagatat tataaacacc tattttcgct cacgggaaac aagagaaatt ctaatgcaac
      961 agcaaattga agtgctttcc gacacttata attctaccaa aggattagat atgccctatc
     1021 tatccccagc gattaaaggc agaattgtga aacgagataa attagtaatc ttagattgtg
     1081 gtggcgacac caatggaatt aaggtgttaa agcaatatta tgatgatatt gttgataaac
     1141 cttatgaaat gtggatggtc atcaatgtct ttcgtccaga aacaagaaat gcggagcaaa
     1201 tacttaaaat gatgcaagca ttaaaaaacg aatcaggatt agaggttact gggtttatta
     1261 ataatagcaa ttatcttaaa agtactacaa tcgaagatat gaaaacttcg aatcaaatca
     1321 tggaagaagt agttgcatta accaagatta aagtagttta tacttcagga gtggaatcat
     1381 taatgcaaca attaccaatt gatatcctag gtgaaaagtt tcccctaaag atattattac
     1441 gagaaaaatg gctttaagga gattaacata tggcaaaggg taaaattgaa gtagcaatag
     1501 aacgttgtaa gggatgtggt ttatgcatag ctgcatgtcc acttgatcta ttagaattag
     1561 atccccattc attaaataca aaaggatacc aacctatgcg cattcataca ccggaaaaat
     1621 gtattggttg cgcaaattgt gcgaccatgt gtcctgatgt agttattaca gtctatcgat
     1681 tggattaata gcatctttag atatgacaat taaaacaaat gaaaaaatta tcttaggggg
     1741 aaaatatata tggcaaaact gatgatgaaa ggaaacgaag cgatggcttt agctgcttta
     1801 aaaggaggtt gtaaagcctt tttcggttat ccgattacac cacaaagtga actcccagaa
     1861 tatctatctc gatatatgaa ggaatatggt ggagtgttct tacaagcaga atcagagatt
     1921 gcagcaatta atatggttta tggtgcaagt gcagcaggcg ttcgagtgat gacctcttcc
     1981 tcttcaccag ggattgcttt aaagcaagaa ggaatcagct atatggcggg ttctgaatta
     2041 cctggtgtta tcatcaatgt gatgcgaggt ggaccaggat taggaggaat tcaaccttct
     2101 caatcggatt ataaacaagt gacacgagga ggagggaatg gagattatta ttgtttagct
     2161 tttgctcctg aatcgcttca agaaacagta gatattatta aagaagcatt tgatttatcg
     2221 gatttatatc gaaatcctgt gatgattgct gttgatggat taattggcca aatgatggaa
     2281 ccagtagatg ttgacaaaga aattaaaaaa cacaaaattt cagaaaaaga ttgggcaaca
     2341 gatggaacta tgggacaaag agaacgacgc attgtaaact ctctttattt aaatactcca
     2401 gaactggaag agcattgtat taaactccac acaaaatatc aaaaaatgaa agaaaacgaa
     2461 cagcgatatg aaatagtgca tggagacgat gcggaaataa tgattgttgc ctatggaacc
     2521 atggcgcgta tttgtcgcag tgcaattgaa attctcagag agcaaggcat cttagttgga
     2581 ttaattcgac cgatatcgat atggccttat ccagagattg cttttgatca attatcacca
     2641 acggtaaagg ggttgttaac ttgtgaatta tcgatgggac aaatgattga tgatgtaaag
     2701 attgccaata aaggacgttt acctatttct ttctttggtc gcagtggagg tatgattcca
     2761 gaaacagaag agattgtgga agcggtcatg aagatgcgag gaggactagt aaaatgagta
     2821 aaatagtctt tgaaagaaca aaagccttaa ctgataatga attttcatat tgtgcgggct
     2881 gcactcatgg aattattcat cgtcttgttg cagaagtatt agaagaatta aatttaatag
     2941 atcaagcaat tgggatatct cctgttggtt gtgccgtaac aaactataat aatttcaatt
     3001 gtgatatggt tcaagcagca catgggcgtg gtccagcagt tgcaacaggt attaaacgag
     3061 taataccgaa tcgtatcgtt tttacttatc aaggtgatgg agacttagca tccattggaa
     3121 ctgcagaaat cgttcacgct gctcatcgag gagaaaagat tacagtaata tttgtgaata
     3181 atgctatcta tggaatgaca ggaggacaaa tggctccaac gacattgata ggacaaaaaa
     3241 catcaacatc tccatttggg cgtacgattg aacaatcggg aatgccaatt agaatgtcag
     3301 aattgctagc aactattcct ggagctgctt atattgaaag agtatcggtt cataatcctg
     3361 ctcatgtaag aaaagcaaag caagcggtga aacgggcatt ccaaacacaa tttgatcaaa
     3421 aaggtttttc tttaattgaa tttatctcta catgccctgt aaactgggga atgacaccaa
     3481 ataaagcatt gaaatgggcg gaagaaaata tgattccata ttaccctctt ggtgtgttta
     3541 gagatgatac aaaggaggtt aaaggatgat ttctgaaaag attatatgtg ctggatttgg
     3601 tggtcaaggc gtcatgctaa tcgggcaatt attggcttat gcagcgaatg aacggggatt
     3661 taatacttta tggtacccct catatggacc agaaactaga ggaggaacgg caaattgttc
     3721 cgtcacaatt tctgaaaacc cagttaattc tccagtcatc tcaaaagcgg ataccataat
     3781 cgtgatgaat aaaccatcgc ttgaaaagtt tgaaaataaa atagcacctc ttgggaaact
     3841 atttatcaac tcatcattga ttgatcaaaa aacaagtaga acagatattg aagcttatta
     3901 catacctgct aatgaaattg cagggaaatt aggtaatttc aaggtagcca atatgattat
     3961 attaggagca tatcttgaaa aaacacactt gtttgatgat cagttgatta ttaaattatt
     4021 agcgaaacta tttggtgaga gtaaagctca cttgatagcg ttgaataatg aagcattggt
     4081 tgcaggaaga aattatttca acaagaatta tcgataagaa agaactatgt tcttttcaat
     4141 gttcttttga acaataatac gagccattta tatggctctt tttattactt gagagaggaa
     4201 acaagaacaa tggttgactg gagtttaatt aagtgataaa attctaatat atgatgagaa
     4261 aacgctctca tttaaacaaa aaagaaagga atttatatgc gttttattag gaaaaattta
     4321 aaggctttta tctttttgtt atccttggtt gcttttgtat tcattgcaat ggcgattgca
     4381 aatggtattc aaaaagattt tggaaacatc gatgttagtg ttggaacgct agaaacaaca
     4441 tttaagacca tggatggtga agaaaaagaa ggagaaattg cttataagtt atatattccc
     4501 aaaacagcaa cttcgactaa tcaagcaagt gctttacttt tattacatgg ttatcaaaac
     4561 gatcatgaaa cctcagctgc ttatgcttta gaattagcaa gaagaggagt tgtagttctt
     4621 gcaattgatg agtttggtca tggagctacc actatcagtt tggttaatcg cggatatgtg
     4681 aaccacaaag ttacagttaa ttatggaact gaatcggaag ataatcaaac atttgttgag
     4741 atagggggac ctacaagata taaaatcatg atgaactttt caaatatgaa gtttttctta
     4801 gataagtatt caaaagatga tgatgggaac caactattag attcttccat gggaggaata
     4861 gctgcttatg catatttgtc tagtttaccc tatgttaata ataaaaagat ggctgtttca
     4921 ggtcattcta tgggaacttg ggcttcatgg agtgtcagtg ctgcttattc tggaaatgag
     4981 atagcaccaa aagcaacgat tcttcaagct ggtgagttgt tcaccaaaga agcatatgac
     5041 agtacgaata ttcactttaa taatgtttta ctattaacag caaaatggga cgaatttaat
     5101 atgttccgcg attatagtaa acaaactgtc aatgattcca ttattcaaaa cgaaataaca
     5161 agcgagtttt taggaatagt tttaggcact ggaaagtgga ataccactta tggtgacttt
     5221 gaaaatggtt ctgcgcgtcg tcgtgaattg atactaacca atcatcgttt attaactcac
     5281 aataaaaaag caattggaac aacgattgat tggcttgatg aaacaattgg tataaataca
     5341 accttggaca atgctaatca agtatttgca atcaaagaag tattggtatt agtagctact
     5401 ttttctgcga ttgtaagtat gtttgcatta ttcatgatac taattaaaat tccattcttt
     5461 acttcaattg ctcaccctga aatcatttta gaaagagcag gtagtgtaaa aacaggttgg
     5521 aaatggtgga aaggtgcgat tattactatt cttatcgccg gattaacgta tccatttatg
     5581 actcaacttg gccatggttt attatcatta ccagaaatga ccgtttttag aatgacaatt
     5641 ggaaatggtt tcttctcatg gtatcttata ttgatcatta ttatgttact aacaacaatt
     5701 cttccttgga gaaaagcaaa gaagttaaat ataccaaagg attattgtga tttaggatta
     5761 tctactcctg aaaataaaga tcgatttgat tgggtattat tagggaaaag tgcaattgtt
     5821 gtcgcttgta tgattctctt tatgtatctt caatgtatta tttgcgagaa agcgttcagg
     5881 ttagacttca gatttatttg gccgttcttt aaaggattta gtggcgaacg atttattcag
     5941 ttgttagtgt atctaccaat attcatctta ttctttgttc taaataattc taaaattttt
     6001 gctcagatga gaaatagtgg cactaataaa ccagggttca agggttttct gtcttgttgg
     6061 tggcgtaatg cattattaat gattggtgga atattattat taatcttagt ggaatacatt
     6121 ccattcttta tgggaattgg tcctggaatg gatttattat ttagttctac ctttggtgga
     6181 ccttttatgt ctttgatgat tgtctttgtt cctcaagtat tagtatttag cttattatgt
     6241 acatttgctt atcgcaaaac aggaagtgtt tttgttggtg ccttaacggt agctgttctt
     6301 gcttgttgga ttatcacagg cggttcagca attctataag aattcataat taatagtaaa
     6361 aggtttagat tcaattcaac ctaaaccttt ttttgtatat aaagttttca gatataagaa
     6421 ataaaaggtc tattacacag cgaaaaatca tttattaagt ttttattata tggtagaata
     6481 gaaatagata ataattaatg atatcgagta agtaggtgaa tcaaatggat aagttatttc
     6541 ctagcgaaat tgatcaaatt gtaacaggaa ttagttatac gattgatgat attggttgta
     6601 gtggtgataa ggtatttctt tttgagaaga agtatgtttt aaagatttct aacaataaag
     6661 aaagactaaa gaatgaaaaa atgaaaatcg attggatagc ttcctatatt ccctcttcta
     6721 aaagcgtggc gttttatgaa gatgaaatga acgggtatta tctaagaacc tatttggatg
     6781 gagatagttt aattcataag cgctttttaa gccatccaat caagttgatt gaaatactga
     6841 gacaagcagt attattgttg aggggattgg atgataaaga atgtccattt caatcaattg
     6901 acaacaaagg cgatgctttt attcatggag atttgtgctt acccaatatc ttggttgacg
     6961 aaaacgatga tattattggc tttattgatc tagataattc tgggaaaggg gatccatggt
     7021 atgattattc atggctattg tggagtttgg agtttaacct taaaaccgat cgatataatc
     7081 ataaattact tcaagcacta ggaatttcgt ttagaaaaga aaaatacgaa caatatattc
     7141 cttttgaata tatatcagat ttgaatagga ataaatagta tagtttagaa gtaataattt
     7201 tacttctaaa aaaaaggtaa aattatatta tagttaagaa tttaagggtt tggtgatttt
     7261 atctattcgc ttctatttga atcaatcctt tgcccttgaa aaaaatttat tatcatgcta
     7321 aaatgtagta gttttgaata aaaggagaaa aacatggaaa aatactttat tttaagtact
     7381 attgtagttt caatagtggc atttgtcttt gctttttggc tatatttatg ggtatcaaaa
     7441 caaccttcaa gcaataagaa agttgctagt ataggtaaat taattcgtaa aggagctaat
     7501 acttttctaa ggaaggaata tattgttctt gctaagtttg caggagtaat tgcaattcta
     7561 atttttgttt ttttacctga accaatttgg aaaggtaata ttcttgctaa tcttacaatg
     7621 ttaatttcct atgtttttgg aactggttta agtgcacttg caggaaaaat cggtattcaa
     7681 gtagctacta ttgctaatgt gaaaacagct gaagctgccc aaaaaggaat caagccaagt
     7741 tttttaaatg gttttagagg tggagcagtc atgggaatgg ctgttgttgg tgcatcatta
     7801 ttaggtattg ctttagtctt tttaatcaca gaaaacagta ccgcattatt aggatttagc
     7861 tttggtgctt caagtcttgc tttatttgca aaagcaggtg gtggtatctt tactaagaca
     7921 gcagatatta gtgctgactt agtggggaaa gttgaattgg gtattcctga agatgatcca
     7981 agaaatccag ctgttatcgc agataatgtt ggggataatg ttggtgatgt agctggaatg
     8041 ggagctgatt tgtttgactc aaatgtagct tcattagcag cggcattagt catggctgtt
     8101 gctttaggtg gagtaactgg taaaaacgta atgatggtat tttgttttgc tacaattggc
     8161 ttactagcat ctgttatagg tgtaggtgca gctaaaatgg gtaaaaaagg aaatcctact
     8221 catgcattaa atatgagcac atatgttaca actgcaatat tcattgcatt aacatttgtt
     8281 gcaacactaa ttttcaaatt tgaatggcgt atatggggag catcagtagt aggtttatta
     8341 gttggtgtta ttatcggtat tacaacggac tactttacaa atgatgagcg taaaccggta
     8401 cgtaatgttg cgaaagctag tgaaagtgga ccagcattta caattatcaa tggtgtaagt
     8461 tatggcttct taagtgtttt accagcaatg gttggtatcg ctatttctgc gttagttgct
     8521 tataaattgt gtgaaccatt aggagcaaac tatggtatgt ttggaatttc aatggcagca
     8581 gtaggaatgt tatccattgt aggcatgatt attagtaatg atgcgtatgg acctattgtt
     8641 gacaatgctc gtggacttgt tgaaatgggt aatttaggtg aaactgctct tgaaatcaca
     8701 gatagtcttg attctgctgg aaatacagtt aaagcagtta caaaaggatt ctctattggt
     8761 gctgctggtt taacagtaat tgctttactt ggagctttta tgagtgaagt aaatgtaaaa
     8821 gcattggaat tgggttatga tttaatcact ggatttgata ttatggatcc agtcatcttc
     8881 tttggtttat tagttggtgc agcagtgcca gcagtattta gtgccttatt aatgctaggt
     8941 gtaaatcgta acgccgaaag aatggtaaaa gaaatccatc gtcaattcca agaaattcct
     9001 gggttagtgg aaggaaaaga aggggttata ccagaatatg ataagtgtat ctctattgct
     9061 actactggag cactaaaaga attaattcca gctggtttat ttgcaattgt agctacaatc
     9121 gttgttggct ttgttggtgg agtaaaggca gttggtggtt tcttagtagg taatatcgtt
     9181 agtggtttat tactagcact attcatgagt aatagcggtg gtttatggga taatggtaaa
     9241 aagtatgttg aatctggaaa ttgtggaggt aaaggaagtt ttgctcataa agctgcagtt
     9301 gttggtgata ctgttggtga tccattcaaa gatactgcag gtccaagtat taacacacaa
     9361 attacagttg ttagtttact agcaagtttg ttaagtacta tcttcctagc attatccatt
     9421 ttctaattaa aaagatagag actttagtat ttcataatta ctaaagtctt ttttaatttt
     9481 aaaaatattt ttaatcatta atttatttaa ataggatatg gtgcaagtat ataaatgaaa
     9541 atggaagaag aaaaaacttt ttaataggat tatttttact tatttaactc ctatcttaga
     9601 ttgttattat taatcaattc acaattatgc acctgttttc atgaaatatc tttaaatatt
     9661 accaagttat aaaaaaaata tacttttctt tttaaataat gatataatat tttatattag
     9721 gtgtttttta tttaatgaat aggaaaggga acaaacacaa acatgaaatt ttggaaaaaa
     9781 attaagtaca aatttgatca attattatca aaaggaacga tttctttagt tctcgcatta
     9841 tttacagtta catttattgc agttgttttg attggactaa ttgcctattg gtcaacaaaa
     9901 agcacagatt cagctcttag catgatttgg actagtttta tgaaaacact agatccaggt
     9961 aatctagaag gagaagaagg aacatttttt tatgttcttt tgatgaccat tgctacaata
    10021 gtaggaatct ttataactag tattttaatc agtattatct caagtggatt tcaaacaaaa
    10081 cttgaaaatt tacaacgagg aacttcacaa attattgaaa agaatcatac tctaattcta
    10141 ggatggaatg ataatgttcc cattattgtt tctcaattaa ttgaagcaaa taaaagcgta
    10201 aaacatccta aaattgctat tttatcagaa caagacatga tggaaacata tcacgcactt
    10261 aaacaactgg tgaagaattt ccacaacaca aaaataatta ttagaactgg atcgcttttt
    10321 gataaaaata gtcttgaaac ctgtgctatt gattgtgctc gttcagtgat tattagtgca
    10381 attgatgatg taaagatagt aaaaacattg ttagcaatca aacaaacatc atttttcaac
    10441 gatttgaaat caggatacat tacagcaatc tttaccaatc caaaaacaat gaaactcgct
    10501 caagaaatgt gcggtgataa acttgttgct cttcacttaa ctgattcaac aaatcgaatt
    10561 atggcacaaa catgcttaca accgggactt tctttggttt atcaggaatt gtttgatttt
    10621 aatgggaatg aaatttattt ttataaaaat aatcaattag ttggtaaaac agttcatgat
    10681 gcgattatga aatttaatga ttccatttta attggtattc aaagaaatca agaaacattg
    10741 attaatccac ccaaagatat gataatttct gaagaagatg aagccatcct tattgctgaa
    10801 gatgaagata aaatttttat taatgaagac aattattcat tatatgaaga caaaattata
    10861 ttaaaaccac ataacaagac aaaatcgatt agaaatattc tttccatagg ttttaataat
    10921 gatacatgta aggttatcaa agaactgtta ccctatatag aaaaaggaac acatgtaaag
    10981 tttttaatcc ctgataattt agatgaacaa ttattcgagg aaatagaaga tcaagattta
    11041 attgagcatg atattataag aggtgtcact tacgaaagag aaactctaga agcgattaat
    11101 tataccaata tcgataccat tattgtattt gcgaacagtc aggatgatgg tgaaagatcc
    11161 gattcagaaa ccctgttgac tgttttaaat ttaaaacaaa ttgaaaaaga aagaaaattt
    11221 aatgcatcga tcattattga aatagaaaaa aatgaaaacg agtatatttt aaaatatgct
    11281 agtgtagatg attttattat tagtaatgta ttggctaaca aaatgttatg ccaaatatca
    11341 gaaaatagaa atctacgaaa agtttttcaa gagttacttg gggaagaagg atcagaaatc
    11401 tatttgaagc acgtagaaga ctatgttacc attaatgaag aagtaaattt ttatactatt
    11461 tctaagagtg catcactgaa gaacgaaata gcaatcggat ataagaagaa agcagtgtct
    11521 gaaaatggag gaattgttat taatccaaag aaagacaagc aagtagtatt ttctaaagga
    11581 gatagtatta ttttaattgc gattgactaa tcctttaaaa atagagaaaa gaggaattaa
    11641 tttatggaaa gcgaaaaaag aaaagaagta attcgaataa ttaagtttac tatcttttcc
    11701 atttcagcgg gtatcattca attggtatct tttacgattc tatcagaatt gtttgatatt
    11761 acttattggc caagttattt aacagcgtta atattatctg ttttatggaa ttttacgatt
    11821 aatcgtaaat ttacattcaa atcagcaaat aatgtgccga tagcaatgac aaaagtattg
    11881 gtttattatg cgatttttac accattatcg acttggtggg gagatgctct cacaaaagct
    11941 aattggaatg agtatcttgt tttaggaggg accatgataa taaactttgt tactgaattt
    12001 ttatatcaac gtttctttgt gtttggcaaa agcatagaat cagctgtccc taataaagaa
    12061 gcatagtaag aaataaaaaa ggaaaggaaa ctttccattt tttattgact ttatattttt
    12121 catttgttat aatgatgtaa agttaacttt acatgaagga gtagataaat tgaaaaatca
    12181 tttagaaaag ctacggcaag aaagaggaat caaacaggaa gacttggcaa aagccatggg
    12241 agtatcacga caaacaatag gttctttgga aaatggaaga tacaatccat ctattttatt
    12301 agcttatcga atagcatact tttttaattt gaaaattgaa gatattttta tatatgagga
    12361 gaagaataat gaagacattt aaaaaaacat attttgaaat tttatttatc atcggtttat
    12421 tactttctgc actggtatca tttgatcgag gttatcactt tcttcctgat tttattgggg
    12481 gaatgttaac tagtttatca attgtattga ttgtatttgc cggaatagta aagcatagta
    12541 agaaattgca agataaatta caaatagctg atagggatga gagattacta acaattgaac
    12601 aaaaagctgc aaagataact ttatatgcta ttatgttcat caatatagtt tgtattatta
    12661 tttttggctt tttaggtgat acttatcttt atatgtcaat tctattggca ataatattat
    12721 tcattacaat tgttatttta tttataacaa aattagtact tacaaaaaga atgtaataaa
    12781 aggagtatat atgggtaaaa tttatgtttt tggtggtggt gaaatttctc aaaaagaaac
    12841 atatctcctt gataaattgg ttgtttcaaa attgaataaa caacatccta aagtattatt
    12901 tattcctaca gcatctcatg atgcagaagg atatgtcgct tcttttactc gaatttacga
    12961 atcacttgga tgtattgttg atagtctttt actagtaaaa gaaaatatta ccgatgatgt
    13021 tataaaagaa aaaatactaa atgctgattt aatttatgtt ggcgggggta atactttatt
    13081 catgatggat acttggagaa gatataaagt ggatgaacta ttgattaaag cctatcatca
    13141 tggtgttgtt ttgagtggcc tttctgcagg gggtatttgt tggtttgatt atggatattc
    13201 tgattcactt attattgaag gaggttatga atatacctat gttgaaggat tacatttgat
    13261 tcatatggat cacaatcctc atgctgaaga ggaagaacgc caaggatttt taaactcttt
    13321 taaaaaaaga aagaaaaaag cacttagtat ggataatcta actttagcgt attttgaaaa
    13381 tggttattta aaaacaggat ataaatgcaa cgagaaagcc aaagcgatac ttgtgcaaag
    13441 tagcggagaa gaagttacat gtgaagaagt ttttcttgaa tttattttag aataaatatc
    13501 ggagaatgag agatgataga acaaccaaaa aatgaatttt tgaaatacga ttttgaaatt
    13561 aaagaaaaca atatctgtga tatctatcaa ttatatgaaa atgtctcatc attttccact
    13621 tgataaattg aaagttgaag aagatgttaa tgctattcca tcaattacgg acgaagatca
    13681 taaatattat ctaagtttat atcaagatga acagttggta gcagtcatcg attttattga
    13741 tggtttcagt tttaaaaagc aaaacaacga aaatacgata tggataggtt tgcttcaaat
    13801 taataaaaca aattaaaacc aaggaatcgg aagtaaaatt gttcaaattt tatgcgacta
    13861 ttttcgtaaa gaaagtaaaa agacgcttca aataggtgtg ataaaagaaa acgaaaaagc
    13921 aatctctttt tggaagaaaa ataactttca agtgattgga gaagtaaata atggggagtt
    13981 tgatttattg atactagaac gtaaaatata gattgttgct tagaacgaaa tttataggtt
    14041 tatctacgat acgtaggttt acttatcgtg tatcaattag tataataata tcatgaggtg
    14101 ataaaaatgg attcttatgt gaccagtaca atgataaaaa aattacgtga agaaaaaagg
    14161 ttaacacaaa aagagttggc agatattatt ttagtgagtg aaaaaacaat ttctaaatgg
    14221 gaaacaggaa agggacttcc cgatattagc ttgttagaac cgttgtctaa agcacttggt
    14281 gtttctttaa ttgaattatt tcaaggagaa gctattaaaa acaaaaataa agcagctaat
    14341 ttactgaaaa ctcgatttta tgtttgtcca gtttgtggta atgtggttac atccattgga
    14401 gaaggatcct tttcttgttg tggtgaaaca ttacttgtac aggaagttga aaaagttgat
    14461 gatattcaaa tagaaagaat tgataatgaa ctatatatta tggtagtatc tcccatgagt
    14521 aaaatagact atatatcttt tattgcttat tgtactactg atagaataca gataaaaaaa
    14581 ttatatccag aacaaaatgc agaagcttta tttgcaatca atggtccagg tttaatttat
    14641 tattataata atagaaatgg cctttttgtg aaaaaagtgt gaagttagtt ttgattcaac
    14701 aaaaaattca agagtaagta aaagaagtga tgaaagtaat gattatagct gatatataaa
    14761 ataaaaaaaa gcattaatga tgttgatgaa acaccttttt ttaaaagaaa tacattgaca
    14821 accatcaata tgaatagtat aataaattgt gttttaatga ggaataagat ggataatcaa
    14881 gattttttaa acaaattaaa aattataaat gatccaacta gattaaaaat catacaatta
    14941 ttagccaaaa acaagacaat gtgtgcatgt aaaattttag aagaactcaa catcacacaa
    15001 ggaactctat cacatcacat gaaatcattg gttcttgctg gttttgttgg ctgtctacgg
    15061 aaaggcaagt ggtgtcatta cacgctacta cctatgaatt ttaaagaaat cattagcgtt
    15121 ttaaacaaat ttaatgaata aaacaagctt aaaaaaagct ttttattttt aatttgatat
    15181 attgatgatt gtcaatatga aaggattaat ctaatatgaa tggttttaat gaaaaaaaag
    15241 aaatagtatt taagaagaag cgtggtttga taataacctt aagtatcttt tttcttgtaa
    15301 tcgttataaa tttattagtt ggttttatat ggggattaga tgctgtaaaa gaaattaggg
    15361 attttattag tagccaaatc ttaggaatga agtggttgaa tattggtgtt gggaaattac
    15421 taatactaat agtaggtgat ttcgcatcag gaacttggca aaatacgttg attgaagcaa
    15481 tccatttctt catttatgat tttattaaaa taggaatatt gctttgtctt ttaattttta
    15541 caatctctta cgtgcaatca tattttccac ctgaaagaac gaaaagaatt cttgggaaat
    15601 ataaaggaat ttgggctaat tttatcggag cactgttagg aacagtaaca ccattttgta
    15661 gttgttcttc tattcctatt tttattggtt ttacaagagc gggactatca agtggcatta
    15721 cttttagttt cttaatctct agtcctttgg ttgatttagg ttcacttatg ttgttagtag
    15781 gtttctttgg ttggaacgtg gcaattattt atgttgttgt tggtctatta ttagcggttg
    15841 ttggaggaac cattattgaa aaaacgggac taggtaagta tgttgaagac tttgttacta
    15901 gtggtagcag tataatcaat gtagatctac cagaattaac tatcaaagat cgattagaat
    15961 atgcacgtga tcaaatgtta caaatatata aaaaggtttt ttggtatatt gttataggag
    16021 tgggaatcgg aagtattatt cataatgtca ttccaagaga gtggattgag ggcttattag
    16081 gagcaaataa gtggtatagt gtttttctag caacattatt aggtgcgcca atgtatgcgg
    16141 atatttttgg aacgattcct attgctgaag ctttatttat caaaggagca gggttaggaa
    16201 caattttagc gtttatgatg agtgtcacgg cattatcatt accatcgatg attatgttac
    16261 gcaaagcagt aaaaccaaaa ctattatttg cttttattag tattgtaatc acaggaatta
    16321 tcataatagg tatcttattt aatctatatg gtaacgtatt tatttaatcg aaataaaaaa
    16381 ttcatgtata aggagaagaa aatgaaaata caagcactgg gtggctgttg taaaaagtca
    16441 caagaaaatt acaaatcaat tgtggaagca gtaaaactat tagaattaga tgttgttgta
    16501 gaacatgtaa ctgatatgaa tgcaattttt gaactaggag ttatggctac tccgggttta
    16561 attgtaaatg gtaaaatatt agtaactggc agaatgctta atgtttcgca agcaaaagaa
    16621 ttaattagta aagaattaaa attatgaaaa aaaatattgc ttttgtatgt gtacataatt
    16681 cctgtagaag tcaaattgca gaagcattcg gtaaattata tttaagtaag ataacaaatg
    16741 tatattctgc tggaacaatt ttagcgaatc aaattaatca agatgcaatt agattaatgc
    16801 gtaaactata tgatattgat cttgaaaaga ctcaaaaacc aaaattaatc tcatctcttc
    16861 caaaaatcga ttatttgata accatgggat gtaatgttga atgcccactt ataccttgta
    16921 aagtacatct tgattgggga attgaagatc ctacttattt tttagaagaa aaattcataa
    16981 aaacaattaa aatgattgag aaaaaggtac tttctttgaa agtagaaata aaaaagcatt
    17041 ttaattgaat ctcttttcaa tatctactat aatatgggtg tacttaaaat acaggaggga
    17101 aatatgttaa aagaaaatgt aactaaatta ttaaatgaac aaattaataa agaaatgtat
    17161 tcagcatacc tttattttga tatggctaat ttttatgtaa acaagggaat gaatggattt
    17221 gcaaattggt ttaatgtgca agcaaaagaa gaaatggatc atgcactact atttgttaag
    17281 tatttacaaa ataacggatt tgaagtttcg ttagcagcta ttgcaaaacc tgataaagtg
    17341 tttaaagact taagagaacc tttagcagaa gcattaaagc atgaagaata tgtgaccgat
    17401 tcaataaata aaatatatac agaagcccta aaggttgctg attatcgtac tcaagaattc
    17461 ttaaattggt ttattaaaga acaaggcgag gaagaaaaaa atgcaaat
//
LOCUS       DAOSGS010000005        17140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_2711_length_17140_cov_5.740416, whole genome shotgun sequence.
ACCESSION   DAOSGS010000005 DAOSGS010000000
VERSION     DAOSGS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2711_length_17140_cov_5.740416"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLQ22_00615"
     CDS             <1..998
                     /locus_tag="PLQ22_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH58495.1"
                     /translation="KNGVNYFDTAATYTDGLNEKFLGQSLKRYPRESYYLATKLSLFR
                     IKEREEIFSFFERQLEQLGVEYVDFYLIHAMNKNIFQKVKDWGIIDILKRWQKEGKIR
                     YIGFSFHDDYKTFKEILDYYHWDFCQIQLNYMDTNFQQGIQGYYDALEKEIPVIIMEP
                     LKGGKLANFNKKVSQLLVDYNPNVSTASWGFRYLFGLEGIKVILSGMNDMEQLDDNLK
                     TFKNPKPLNQEEQKLIKTVESKLNEIFEVDCTQCRYCMPCPAGVDIPENFEIFNDYAM
                     YQAVHNTKWNINLLKKRDALYDACIECGVCLSLCPQNIDIPNELKRMKETLGFIK"
     gene            995..1756
                     /gene="rsmA"
                     /locus_tag="PLQ22_00620"
     CDS             995..1756
                     /gene="rsmA"
                     /locus_tag="PLQ22_00620"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOH58496.1"
                     /translation="MIEAKISLGQNFLVDQNIINKIVNSIPINKDSLVIEIGSGMGAI
                     TKPVYELTKNVIAIELDQRLVDYLNKDFPELKIINEDALKVDIDSLINKAKYANVILV
                     SNLPYYITSPLIEKILKLKKIDYAFLMMEKEVASKIKAKEKNVLSLLVDLRGKIEVLF
                     DIGPNAFKPKPKVVSSFVKISYLYENELDEEFYLFLNNLFLNKRKTLINNLNAIYKKD
                     KKELEFIFEKLNLDKKIRSEKLGIDEIIKLYKELF"
     gene            1756..2544
                     /gene="ispE"
                     /locus_tag="PLQ22_00625"
     CDS             1756..2544
                     /gene="ispE"
                     /locus_tag="PLQ22_00625"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOH58497.1"
                     /translation="MKKKAYGKINLSLHVYEKREDGYHNIESLITLIDIYDKMKFKKA
                     LETKVFVKKAKIKNNIVNKTIELIRKKYNLKESIEVKIEKRIPIGAGLGGGSSNAAAT
                     INAMDELYDLKMTSVDKKLLAEQLGMDVAFFLKNDTCLVRIRGEVATPTINPYVGYYC
                     LLLFSDFSISTREIYEAYVLGDEHLENDLESSYKTTNSLLYKEIQNLKKDLLDLKAVK
                     AMMSGSGPTVYGLFEKKKYARKAAKVLKKKYNRKPIVTKIIKNG"
     gene            2622..2888
                     /locus_tag="PLQ22_00630"
     CDS             2622..2888
                     /locus_tag="PLQ22_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOH58498.1"
                     /translation="MPITNVQVYLVENAGKIKASATITFDDAFVVHGLRIIEGEKGLF
                     VAMPTRYSRKKGRFLDTAHPISNKARSVLVDAILKAYREEVKKS"
     gene            2990..3961
                     /locus_tag="PLQ22_00635"
     CDS             2990..3961
                     /locus_tag="PLQ22_00635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH58499.1"
                     /translation="MATIHGKKIKLFSLSANPELAQEIAECMGTGLSDCTITRFADGE
                     ISVNIGETVRGHDVFVIQPTSAPANDHYMELLIFVDALKRASARTINVVMPYYGYSRQ
                     DRKALARQPISAKLMADLLQAAGITRVVCMDLHAAQIQGFFDVPIDNFEALPIMVQYL
                     KKKKLQDLVIVAPDHGGASRARKFANCFNAPLAIIDKRRPSPNVVEVMNIIGDVEGKN
                     AVIVDDIIDTAGTLTEAARALVKAGAKEVYAVATHPVLSGPAIERIQKSPIKELITTN
                     SIRLPEEKRIAKIKQLSIAKQISQGLINIIDDKSVSALFQYKPEQNF"
     regulatory      3998..4099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4287..5651
                     /locus_tag="PLQ22_00640"
     CDS             4287..5651
                     /locus_tag="PLQ22_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOH58500.1"
                     /translation="MEEKRLVLDIEQKPKKIRHWLLFATQHFLAMIVACITVPIITGL
                     PIDATIVAAGIGTLFYILVTRGKSPVFLSSSFAYIAPMLSALALGDEIAKNNLLAVII
                     GMGIVGVVYVIVALIVKFVGTKWLDKLLPPIVIGPVIMVIGLGLSASAVGNLSTTANN
                     YNIVGIAVGLVAMLVTALVAHFGKGTVKLVPFVVGLGAGYVLAAGLTAIGNAADIEIL
                     KLINFTPLKEAFSPVKFESFLHLPDFLFLKNMTSQFNASQIPTIILLFAPVSFVTICE
                     HIGDHKNLGNIVGRDLLKEPGLTRTLIGDGVATSISGILCGAANTTYGENTAVVGVTK
                     IASTAVIGGAALLSVFVGFFTPFTAFVETIPGSIMGGVSLLLYGFIAASGMKMLINER
                     IDLGVNRNIFVVAVILVAGIGGLVLRVGVVEITSTAVAMILGILLNLILNPKKEAKME
                     DYDV"
     gene            5852..8440
                     /locus_tag="PLQ22_00645"
     CDS             5852..8440
                     /locus_tag="PLQ22_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH58501.1"
                     /translation="MKRAFKVLGTIVLIFFVTFFSYGCKEKLKTSLEKDKVTIALREN
                     LKVDILSETSEEVIWETEDASIATVENGVIVGVSKGTTYITITSGELSAKIKVTVSDE
                     DGKYIVTWLNYNGDLLELDIDVPHGTMPSYDGEIPRRGADFVFKGWDPKVEPVKGHIT
                     YTARFEEINTHTVTWKNWDGSTLAVDNAVVSGAMANYSGPAPTKKSDERYDYMFMGWS
                     PAPGQVTYNMTYTAQFTAIQKTFKVTWQDQYGTVLKVEEGVFAGSYASWDGPVPTIHD
                     NPQTGVKRLFKHWLPSPGAAPITEDTTFTAYFVDAYLVSFVSEGVVLQEEHVRKGEMP
                     EFIGQEPRKINDPNDTKYFYTFKEWFPAIEEVKSKNQTYMATYDCFPKSGYGHLSIDE
                     EFEIDTYDYRYQEYGRYAVLKKYKLKDSGVEATIPAWYKGYPILQIGYKAFAESQKLE
                     KLICSDTAIWVAEGMCVDCPALETFVFGKKLTRVYTRMFEKCVSLTDFVVPEGIEKID
                     WRAFNECENLKLITLPSTLTEIDNSTFAQCKRLVSIHIPASVTNIGNWTFTGCEDLVE
                     VTFGEDSKLIGIGKCTFQDCTSLKYIEIPALSSCIYDYAFAGCESLVQVDIAPEGMTL
                     IGEGAFQDCVSLHRVIFPETLEEIDEKAFRNCDSLFSIDIPASVQKLEGAFSECKNLS
                     FIDVSGQNPKYDSRNDCNAIIETATNKLIKGCKNTKIPNTVEIIGRHAFFRTPFTSIT
                     IPASVKTIESYAFYYCHDLPHYNYTDADYDKGQLKHDHSYYCKISLFIPKTVETIQNS
                     GIIFDTHFMGPYSETGIIGSQFCIHTDAKKGEKPNWHDHSDVYGHTYWCSGFYVSVHW
                     ADGTSW"
     gene            8489..11092
                     /locus_tag="PLQ22_00650"
     CDS             8489..11092
                     /locus_tag="PLQ22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH58502.1"
                     /translation="MKKTFKVLGTIILVFFVTFFSYGCKEKLKLSLEKNEVTIALGEN
                     LLVDVLSETSEKIVWETEDASIATVENGVIVGVSKGTTYITVTSGDLSAKIKVTVSEE
                     DVYYTVTWLNYNGDLLELDVSVPQGTMPSYDGEIPTRGADFVFKGWDPKLEPVTKNVT
                     YTARFEEINTHTVTWKNWDGSTLAVDNAVVSGAMANYSGPAPTKKSDERYDYMFMGWS
                     PAPGQVTYNMTYTAQFTAIQKTYTVTWKTFEGTILKVEEGVFAGTYASWDGPVPTIGD
                     DPQTGAKRLFDRWSPNPGSATIKEDTTFTAYFKDACLVKYISEGVVLQEEYVKENDEH
                     VYKGAEPRKIDDPNDTKYFYPFKEWILNDRYSYMDNAYVYQATYDCFPKPGYYDVRPQ
                     DEFEFIIHEGSSQYEYDHGYAVISGWNLKETSEEVTIPAWYDGYPVMQIYPNAFQQSP
                     KLKTVIFSDTVRDFHQDEFKGCLVLENIVFGKSMTYIGRSMFANCPRIVTLDIPEHII
                     EMGSYAFENCTGLQVVNLPDTFEEFESGEFSGCTRLTSMRIPAKVEHLSYTFHDCTAL
                     KYVTFAEDSNLKSLCAFYNCISLEYIELPASVRTIYNSCFNGCEKLEEIYLPDEGVEQ
                     IFDYAFHDCVALRDIRLPKTLKFIGREAFENCQSLFSLDIPYQVREIGENAFLNCLGL
                     SLISVDGNNPVYDSRGGCNAIIETESKKLIYGCKNTTIPDGVEIIGRDSFYKVGITRI
                     NIPASVKTIETHAFYHCHDLPHHTYKASDFKNGDGNGPLEHEHFAYCVTSIYIPNTVE
                     TIQINAIVWDHAMTGPTIGRGQYCIRTNATTTEKTGEAWHNYSNVYGHTYWCSGYLIY
                     IHWADGDRVIW"
     gene            11287..13983
                     /locus_tag="PLQ22_00655"
     CDS             11287..13983
                     /locus_tag="PLQ22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH58503.1"
                     /translation="MKKIGGILVMLFLAVIFLSACSCGEEKLYTITWKNHDGTVLEVD
                     EGVKEGTVPKYDGAVPTKEGDENNAYTFSGWSPAVKKATEDATYIAQFTARESKYTVT
                     WKNYDGTVLEVDEDVPYDTMPEYNGATPTKEGHTFIGWEPALSKVKVNIEYTAKFKVS
                     GGTGEPTSTEDFVFADVGEGYALVHYFGNASDVVIPGEHKGKPIISIQKWAFFANDTM
                     KSLVIPDSVVDIKEDAFAQCKILERITFGSGLKTIGMMAFDSCLALEAVNLPEGFVHL
                     DIMVFSNCPALTSVSLPNSIESFGEYMFDSYEEPSTVTYNEYANGRYLGNATNPHLVL
                     VDMIDKEATSFTIHNNCKLISDGALSKSNIETINFGSGVKIIGGNAFKNCKGLTELII
                     PEGIIKIGISAFSQSSVTKVTIAESVKYIGLYAFESCSQLTTVSLPNGLEFIGPYAFD
                     YNDNLITTDYDGCNYLGNSDNPHLLLLEARDKSKNNIEIHKDTKMIADVAFARCRNLT
                     TITIPKNVKFLGKNLFYDSNNLTEVIVEEGNIVYEAPNNTVIIEKATKTVVLGLNNAV
                     IPEGVTKIGDDAFNNCSQLETIIIPEGVTSIGDSAFSYCMKLESVTIPNSVTHIGRSA
                     FSDCYKLGSLTIPNKLKYIGHWAFHRCHSLTTLVVPDSVSYIDEYAFGNCEGLENLTL
                     GSSLVVIGETAFSGCEVLQSITIPDSVKTIESSAFSGCSAVTSIKIGKGVISINPYAF
                     LGCKGVATATVDSENKVYDSRENCNAIIETEPNILVFGTKATIIPDSVTEIGRYAFYE
                     CVNLENLELSDNITAIGEYAFYRCSGLNAIVIPESVVNVGRYAFRRCTNLKIFVETTE
                     LPETWDSNWNEGTPVYWYSEAPNYNGSHWHYVDGVPVIWVQQ"
     gene            14080..16860
                     /locus_tag="PLQ22_00660"
     CDS             14080..16860
                     /locus_tag="PLQ22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH58504.1"
                     /translation="MKKISGILVMLFLAVIFLSSCSCGEAEYYTVTWKNRDGTVLEID
                     ENVEEGTYPVYDGEEPTKENEEHYTYSFNGWSPAVTYVEEDVTYIAQFKKTPIEYTIT
                     WKNWDGTVLETNEQAAYDSIPQYKGATPTKEGYTFDGWEPAISKVVGNATYTAKFKVK
                     EDGGGGGGGDEGGTESLETEFVFADLGDGYTVVHYLGNANKVSIPEYYMEKPVISIDR
                     WAFNSNKTMTKLIVPNSIQEIKKDAICNCSALKTVTLGNGIKTIGANAFGICPLLETI
                     NLPEGLTYIGDEAFRECPKLRSMSLPNSIESFGKAMFKGYDNMEYTTYEGGKYLGNNG
                     NPYLVLVGMSSSWNTSITIHNKCKLIANGAFEKINFTELNFGTGVKIIGKLAFSQCKG
                     LTEITFPDSLIKIDDNAFSQSSITKVTFGNNLRYIGNNAFERSRSLVSVSIPNSIEYM
                     GIYAFYQHDNLATTEYNNAKYLGNGENPYLVLLKAKNTSINNIEIHENTKIIAGFAFE
                     GCSSLTTITIPKNVKYIGRSAFQACNGLNRIIVDEDNTVYAAPNESAIIEKLTSRLAF
                     GFNETIIPNGVEIIGAHAFYNNTQITAITIPEGVKTIEDSAFLACTNLERVTLANSIT
                     YIGNSAFAECLNLGAIRLPNNLKVIEESAFYRNRLVTEVVIPNTTTYIGDNAFYYCGT
                     LRNLTLGNSVVVIGKDAFKGCSTLFHIVIPDNVQSIKYDAFGECTGTLTITIGSGVRF
                     MQKPGFSACNNATALTVDSENQVFDSRENCNAIIETATNTLLYGIKISTIPADIEAIG
                     DEAFNGSQITTLELSGNVKSIGRNAFYNTWLTSVTLEEGLVSIGEYAFGRCLWLGQIV
                     IPRSVTTIDHYAFDSSTSLNIYAKATDKPAGWEYWWNSSNLPVYWYSETPNYDGNHWR
                     YVTGAPTIWVSE"
BASE COUNT         6138 a         2210 c         3231 g         5561 t
ORIGIN      
        1 ataaaaacgg agttaattat tttgatacag cagccacgta tacagatggt ttaaatgaaa
       61 aatttttagg ccaaagtcta aaaagatatc caagagaaag ttattatcta gctactaaat
      121 tatctttatt tagaattaaa gaaagagaag aaatttttag tttttttgaa agacaattag
      181 aacaattagg tgttgaatat gttgattttt atttaattca tgcaatgaac aaaaacattt
      241 ttcaaaaagt taaagattgg ggaattattg atattttaaa aaggtggcaa aaagaaggta
      301 agattagata tattggtttc tcttttcatg atgattataa aacttttaaa gaaattttag
      361 attattatca ttgggacttt tgtcagatcc aacttaacta tatggacact aatttccaac
      421 aaggaatcca aggatactat gatgctttag aaaaggaaat tcctgtaatt attatggaac
      481 cattaaaagg aggaaagcta gctaatttta ataaaaaggt aagtcaattg ttagtcgatt
      541 ataatcctaa cgttagtact gcaagttggg gttttagata tctatttggt ttagaaggca
      601 taaaagttat cttaagtggt atgaatgata tggagcaact agacgataac ctaaaaactt
      661 ttaaaaatcc taaacctttg aatcaagaag aacaaaaatt aattaaaaca gttgaatcta
      721 agttaaatga aatttttgaa gtagattgta ctcaatgcag atattgtatg ccttgtcctg
      781 cgggagtaga tattcctgaa aactttgaaa tttttaatga ttatgcaatg tatcaagcag
      841 ttcataatac taaatggaat ataaatcttt tgaagaaaag ggatgcttta tatgatgctt
      901 gtatagaatg tggtgtgtgt ttgagtttgt gcccacaaaa cattgatatt ccaaatgaac
      961 taaaaagaat gaaagagact ttaggattta ttaaatgatt gaagctaaaa taagtttagg
     1021 gcaaaacttt ttagttgatc aaaacataat taataaaata gttaatagca ttcctattaa
     1081 taaggatagt ttggttattg agattggttc aggaatgggt gctattacta aacctgtcta
     1141 tgagttaact aaaaatgtaa ttgctattga attagatcaa agactagttg attatttaaa
     1201 taaagatttt cctgaattaa agataattaa tgaagatgct ttaaaagtag atatcgattc
     1261 tttaataaat aaagcaaaat acgctaatgt tattttagta agcaatcttc cttattatat
     1321 aactagccca ctaatagaaa aaattttaaa gttgaagaaa attgattatg cctttttgat
     1381 gatggaaaaa gaagttgctt caaaaattaa agcaaaagaa aaaaatgttt tgtctttact
     1441 tgtagactta agaggcaaga tagaagtttt atttgatatt ggtccgaatg cttttaaacc
     1501 gaaaccaaaa gtagttagtt catttgtgaa gatatcttat ttatatgaaa atgaattaga
     1561 tgaagaattt tatttatttt tgaataattt atttttaaat aaaagaaaaa ctttaatcaa
     1621 taatttaaac gccatatata agaaagataa aaaagaattg gaatttattt ttgaaaagtt
     1681 aaacttagat aaaaaaataa gaagtgaaaa attaggtatt gatgaaataa taaaactata
     1741 caaggagttg ttttgatgaa gaagaaagct tatggaaaaa ttaatctttc tttgcatgtt
     1801 tatgaaaaac gtgaagacgg atatcataat atagaaagct taattacctt aatagatatc
     1861 tatgacaaga tgaaatttaa aaaagcctta gaaactaaag tgtttgtaaa gaaggctaaa
     1921 attaaaaata atattgtaaa taaaaccatt gagttaatta gaaaaaaata taatttaaaa
     1981 gaaagcatag aagttaaaat agaaaaaaga attcctattg gtgcgggttt agggggcggc
     2041 agtagtaatg ctgctgctac tattaatgcc atggatgaac tttacgattt aaagatgact
     2101 tcagtagata agaaattact ggcggaacaa ttaggaatgg atgttgcttt ctttttaaaa
     2161 aacgatactt gtttagtaag aataagggga gaagtagcaa ccccaacaat taatccttat
     2221 gtgggttatt attgtttatt actatttagt gatttttcca tttcaacaag agaaatatat
     2281 gaagcgtatg tattgggtga tgaacattta gaaaatgatt tagaatctag ttataaaaca
     2341 actaattcat tattatataa agaaattcaa aatcttaaaa aagacttgtt agatttaaaa
     2401 gcagttaagg ctatgatgag tggatctggg cctactgttt atggtttatt tgaaaagaag
     2461 aaatatgcca gaaaagcagc aaaggtttta aagaaaaaat acaatagaaa gccaatagtt
     2521 actaaaatta ttaaaaacgg ataaaaacta aaaacgtttt caaaaagttc ttgctatttt
     2581 acaaataaat gttataatat aaatagaaag aggaaagaaa aatgccaatt acgaacgtac
     2641 aagtttattt agtagaaaat gctggcaaga ttaaagcttc agcaacaatc actttcgatg
     2701 acgcttttgt agttcacgga ttgagaatta ttgaaggcga aaaagggtta tttgtggcta
     2761 tgccaacaag atactcaaga aagaaaggta gatttttaga cacagctcat ccaatttcaa
     2821 acaaagcaag aagtgtatta gtagatgcca ttttaaaggc atatcgagaa gaagtaaaaa
     2881 agagttaaag caaaacccct ttagataaaa ggggttttaa ttttagaatt aaattgaaaa
     2941 agtgatattt atatagtata atataggtat attggaggta atttaagaaa tggcgactat
     3001 tcacggaaag aagataaaat tattttcttt aagtgccaat cccgagcttg cacaagagat
     3061 tgcagagtgc atggggactg gtttgtctga ctgcactatc acaagatttg ctgatggtga
     3121 gattagtgtt aacattgggg aaacggtaag gggacatgat gtttttgtaa ttcaaccaac
     3181 atctgctcct gcaaacgatc attatatgga gttacttatt tttgtagatg cgttaaagag
     3241 agcatctgca agaacaatta atgtagttat gccttattat ggttattcaa gacaagatcg
     3301 caaagcctta gcgagacaac ctatttcagc taaactgatg gctgatttgt tgcaagctgc
     3361 aggtattaca agagttgttt gtatggactt acatgcggca caaattcaag ggttctttga
     3421 cgttccgatt gataattttg aagcattacc aattatggtt caatatctaa agaaaaagaa
     3481 attacaagat ttagtaattg tagcacctga tcatggggga gcttcaagag caagaaagtt
     3541 tgcaaattgt tttaatgcgc cacttgcaat tatcgataaa agaagaccat cacctaatgt
     3601 tgtagaagta atgaatatta ttggtgatgt agaaggcaaa aacgcggtta tcgtagatga
     3661 cattatagat acagcaggaa cattaactga agcggctaga gcgttagtaa aagctggcgc
     3721 taaagaggtt tatgcagttg caactcatcc agtattatct ggacctgcaa ttgaaagaat
     3781 tcaaaaatca ccaataaaag agttaataac tacaaattct attcgtttac cagaagaaaa
     3841 aagaattgct aaaataaaac agttatcaat tgctaaacaa attagtcagg gtctaattaa
     3901 tattattgat gacaaatcag taagtgcgtt gtttcaatac aaacctgaac aaaactttta
     3961 aaaagttctt gctttttaaa aagctttgtg atacactcta taagataagt aaaaaggtcg
     4021 tatatattca ataatatgga ttgaaagtct ctaccccgaa accgttaatt ttgggactac
     4081 gaatttattc taagtatagg ggcttttaag gagccctttt ctttttggag gttacttatg
     4141 acttatagag aaagagttgt ttgcaataac gtagctaaac cttgttctaa ggctgctttc
     4201 gcattccctt ctcctgtttt tacttttcaa aatttattta aggtcttata attaagacct
     4261 ttttttctac caaaaggagg tttattatgg aagaaaaaag actggtttta gacattgagc
     4321 aaaagcctaa gaaaattagg cattggttgt tatttgcaac acaacacttc ttagcgatga
     4381 ttgtagcatg tattactgtt cctattatta cgggacttcc aattgatgca actatcgtag
     4441 ctgcaggtat cggtacttta ttttatattt tagtaactag aggaaaatcg cctgttttct
     4501 taagtagttc gtttgcttat attgccccaa tgttatcagc actagcttta ggagatgaaa
     4561 ttgcaaaaaa caatttacta gctgtaatta ttggtatggg tattgtaggt gttgtttatg
     4621 taattgtggc attaattgtt aagtttgttg gaactaaatg gttagataaa ttattaccac
     4681 caattgtaat tgggccagta atcatggtta ttggattagg tttatctgct agtgcagttg
     4741 gcaatttatc tacaactgcg aacaactata atatagtggg tattgccgtt ggtttagttg
     4801 cgatgcttgt aactgcactt gttgctcact tcggaaaggg tactgtgaag ttagttccat
     4861 ttgtagttgg tttaggagcg ggatatgttt tagcagcagg ccttactgct attggtaatg
     4921 cagctgatat agagatttta aaactaatca attttacacc attaaaagaa gcatttagtc
     4981 cagtgaaatt tgaatctttc ttacacttac cagatttctt attcttaaag aatatgactt
     5041 cacaatttaa tgcatcacaa atcccaacaa tcattctatt attcgcacca gtttcgtttg
     5101 taactatttg tgaacatatc ggagatcata aaaacttagg taatatcgta ggtagagatt
     5161 tacttaaaga gccaggtcta acaagaacat taattggtga tggtgttgct acatcaattt
     5221 ctggtattct ttgtggagca gctaatacta cttatggcga aaacacagca gtagtaggag
     5281 ttactaaaat tgcttcaact gcagttattg ggggggcggc tttattaagt gtatttgtag
     5341 ggttctttac tccatttacg gcgtttgttg aaactattcc gggatctatt atgggtggtg
     5401 tatctttatt attatatgga tttatcgcag ctagcggaat gaagatgtta ataaatgaaa
     5461 gaatagattt aggcgttaat agaaatattt ttgtagttgc tgttattcta gttgctggta
     5521 tcggcggttt agtattaaga gttggagttg tagaaatcac atctacagca gttgcaatga
     5581 ttttaggtat tttattaaac ttaatcttaa accctaaaaa agaagcaaag atggaagatt
     5641 acgacgttta gatatgaaaa tatacaaggt tattaagaaa ttaataacct ttttattttt
     5701 tttatttaat tttttaaaaa aattaagtag gtaattttaa gaaaacctat gaaaaggctt
     5761 gcactttagc acattacttg ctaaaatatg tatgagtata taaaatgccg aaaaatgtat
     5821 tcaatttata aaactcaaaa gggaggcaac aatgaaaaga gcgtttaaag tgcttggcac
     5881 aattgtactt attttctttg taacattctt tagttatggt tgtaaagaaa aattaaagac
     5941 ttctctagaa aaggacaaag ttacaatagc tttaagggaa aacctaaaag tagatatact
     6001 tagtgaaact agtgaagaag taatatggga aacagaagat gcaagtattg caactgttga
     6061 aaatggagta atagttggtg tttcaaaagg aacaacatat ataactatta caagtggaga
     6121 gttaagtgca aaaataaaag taaccgtctc tgacgaagac ggtaaatata ttgttacttg
     6181 gctcaattat aatggagact tactagaatt agacatagat gttccgcacg gcacaatgcc
     6241 ttcttatgat ggagaaatcc caagaagagg agcagatttt gtttttaaag gttgggatcc
     6301 aaaagttgaa ccggtgaaag ggcatattac ttacacagct cgattcgaag aaattaatac
     6361 acatacagta acttggaaaa attgggatgg atctacattg gctgtagata acgcagtagt
     6421 atcgggtgca atggctaatt atagtggacc tgcccctaca aaaaaatctg atgaacgtta
     6481 tgattatatg tttatgggat ggagtcctgc cccaggacaa gtaacttaca atatgactta
     6541 tacagctcaa tttacagcta tacaaaagac ttttaaagtt acttggcaag accaatatgg
     6601 aacagtttta aaagttgaag aaggtgtttt tgccggttca tatgcgagct gggatggtcc
     6661 agtacctaca atacatgata atccgcaaac cggagttaaa aggttattta aacactggct
     6721 tccgtctcca ggagcagctc ctattacaga agatactaca tttactgctt actttgtaga
     6781 tgcatatctt gtgtcatttg ttagtgaagg tgttgttcta caagaagagc atgtaagaaa
     6841 aggagaaatg ccagaattta taggtcaaga acctaggaaa ataaatgatc caaacgatac
     6901 taaatatttt tatacattta aagaatggtt ccctgcgatt gaagaagtta aatccaaaaa
     6961 tcaaacatat atggcaactt atgattgttt tccaaaatca ggatatggtc atttatcaat
     7021 tgatgaagaa tttgaaattg acacatatga ctatcgttat caagaatatg gaagatatgc
     7081 cgtgcttaag aaatacaaac taaaagatag cggagttgag gcaactattc ctgcttggta
     7141 taaagggtat ccaattttgc aaataggtta taaggcgttt gctgaatcac aaaaactaga
     7201 aaaacttatt tgttctgata ctgctatatg ggttgctgaa ggtatgtgtg ttgattgccc
     7261 agcattagaa acttttgttt ttggaaagaa actaacacga gtttataccc gtatgtttga
     7321 aaaatgtgtg agtttaactg attttgtcgt accggaaggt attgaaaaga tagattggcg
     7381 tgcatttaat gaatgtgaaa atctaaaatt aattactttg ccatcaaccc ttacagaaat
     7441 tgataacagt acatttgcac aatgtaagag gttagttagc attcacatac cagcttcagt
     7501 aacaaatata ggtaactgga catttacagg ttgtgaagat ttggttgaag ttacttttgg
     7561 ggaagatagc aaattaatag gtattggtaa atgtacattt caggattgta ctagtttaaa
     7621 atatattgaa ataccagcat tatctagttg tatttatgat tatgcatttg cgggttgtga
     7681 aagtttagtt caagttgata tagctccaga aggaatgact ttaattggcg aaggagcttt
     7741 tcaagattgt gtatctttgc atagagttat attccctgaa acacttgaag aaatcgatga
     7801 aaaggcgttt agaaattgtg atagtctttt ctctattgac atacctgctt cagttcaaaa
     7861 attagaggga gctttttcag agtgcaaaaa tctttctttt atagatgtaa gtggacaaaa
     7921 tccaaaatat gattctagaa atgattgtaa tgcgattata gaaacagcaa caaacaaatt
     7981 aattaaaggc tgtaaaaaca caaaaattcc taacactgta gaaataattg gaagacacgc
     8041 tttctttagg acgcctttta caagcataac cattccagca agcgttaaaa caattgaatc
     8101 ttatgctttt tattattgtc atgatcttcc tcattataat tatacagatg cagattatga
     8161 taaaggacag cttaaacatg atcatagtta ttattgcaaa atttcgcttt ttattcctaa
     8221 aactgtagag acaattcaaa atagcggcat tatttttgat acacatttta tggggcctta
     8281 ttccgaaacc ggtataattg gatcacaatt ttgtattcat actgatgcaa aaaaaggtga
     8341 aaaacctaac tggcatgatc attctgatgt ttatggacac acatattggt gtagcggatt
     8401 ttatgttagc gttcattggg cagatggaac aagttggtaa tttatttaat ctaacgaata
     8461 tttttaaaat ataaaaagag aggaaactat gaaaaaaaca ttcaaagtgc ttggcacaat
     8521 catacttgtt ttctttgtaa cattctttag ttatggttgt aaagaaaagt taaagctttc
     8581 tctagaaaaa aacgaagtga ctatcgcttt aggtgaaaat ctgttagtag atgtacttag
     8641 tgaaactagt gaaaaaatag tttgggaaac agaagatgca agtattgcaa ctgttgaaaa
     8701 tggagtgata gttggtgttt caaaaggaac aacatatatt actgttacaa gcggtgactt
     8761 aagtgcaaaa attaaagtca ctgtgtcaga agaagatgtt tattatactg ttacttggct
     8821 taattataat ggagacttac tagaactaga tgtaagtgtt ccacaaggaa caatgccatc
     8881 ttatgatgga gaaatcccaa caagaggagc agattttgtt tttaaaggtt gggatccaaa
     8941 gttagaacca gtaactaaaa atgttactta cacagctcga ttcgaagaaa ttaatacaca
     9001 tacagtaact tggaaaaatt gggatggatc tacattggct gtagataacg cagtagtatc
     9061 gggtgcaatg gctaattata gtggacctgc ccctacaaaa aaatctgatg aacgttatga
     9121 ttatatgttt atgggatgga gtcctgcccc aggacaagta acttacaata tgacttatac
     9181 agctcaattt acagctatac aaaagactta tacagttaca tggaaaactt ttgaaggaac
     9241 aattttaaaa gttgaagaag gtgtttttgc tggtacatat gcaagttggg atggtccagt
     9301 acctacaata ggtgatgacc ctcaaacagg tgctaaaaga ttatttgatc gttggtctcc
     9361 aaatccaggt agtgctacta ttaaagaaga tactacattt actgcttatt ttaaagatgc
     9421 ttgtttagta aaatatatta gtgaaggtgt tgttcttcaa gaagagtatg tgaaagagaa
     9481 cgacgagcac gtatataaag gagcagaacc tagaaaaata gacgatccaa acgatactaa
     9541 atatttttat ccatttaaag agtggatttt aaatgatcga tatagctata tggacaatgc
     9601 ttatgtatat caagcaacat atgactgttt tccaaaacca ggatattatg atgtacgtcc
     9661 acaagatgaa tttgaattta taatacatga aggatctagt caatatgaat atgatcatgg
     9721 ctatgctgta ataagtggat ggaacttgaa agaaacgagc gaagaagtga ctattccggc
     9781 ttggtatgat gggtatccag ttatgcaaat ttaccctaat gcatttcagc aatctccaaa
     9841 actaaaaaca gttatttttt ctgatactgt tcgagatttc catcaagatg agtttaaagg
     9901 ctgtcttgta ttagaaaaca ttgtttttgg taagagtatg acatatattg gtcgcagtat
     9961 gtttgcaaat tgtccgcgaa tagttactct tgatatacca gaacacatta tagaaatggg
    10021 atcatatgca tttgaaaatt gtacaggatt acaagtggtt aatttaccag atacttttga
    10081 agaatttgag tctggagaat tttcaggttg tacacgtcta acaagcatgc gtataccagc
    10141 aaaagtagaa caccttagct atacatttca tgattgtaca gctttaaaat atgttacttt
    10201 cgcagaagat tctaacttaa agagtttatg tgcattttat aattgtataa gtttagaata
    10261 tattgaacta ccagcatcgg ttcgtacaat ttataatagt tgttttaatg gatgtgagaa
    10321 attagaggaa atttacttac cagatgaagg agttgaacaa atttttgatt atgcatttca
    10381 cgattgtgta gccttaaggg acattagatt acctaaaact ctaaaattta ttggtagaga
    10441 agcatttgaa aactgtcaat ctcttttctc attagatatt ccatatcaag ttagagagat
    10501 aggagaaaat gcgtttctta attgtttggg tctttctctt atatcagtag atggtaacaa
    10561 tccagtatat gattcaagag gaggttgtaa tgcaattatt gaaacagaat ctaaaaaact
    10621 tatttatggt tgtaaaaaca caacaattcc tgatggcgta gaaataatag gaagagattc
    10681 gttttataaa gtaggtataa caaggataaa tattccagct agtgttaaaa caatagaaac
    10741 tcacgctttt tatcactgtc atgatcttcc acatcatact tataaggctt cagattttaa
    10801 aaatggtgat ggcaatggtc cacttgaaca cgaacatttc gcatattgcg taacttcaat
    10861 ttatattcct aacactgttg aaaccattca gataaatgct attgtttggg atcatgctat
    10921 gacaggccct accattggaa gaggacaata ttgtattcgc acaaatgcaa caacgaccga
    10981 aaagacagga gaagcttggc ataattattc taatgtttat gggcatacat attggtgttc
    11041 tggttatttg atttatattc actgggcaga tggagataga gtaatatggt agtattttaa
    11101 tatacgccat ttatctgatg attattttaa gaattaacaa tagaatatta ttgaaactta
    11161 atgtaagtca ataatataaa taaaagcaca taagtataag agaataaaat ttacttatgt
    11221 gctttatttg tttgaaaagt atataacaat acaccattta atatatcatt aaacaaagga
    11281 ggttttatga aaaaaatagg tgggatttta gttatgctgt ttttagcagt tatatttctt
    11341 tcggcttgta gttgtgggga agaaaaactt tatacaatta cttggaagaa tcacgacggt
    11401 acagttttag aagttgacga aggagttaaa gagggaaccg tgccaaaata tgatggagca
    11461 gtaccaacta aagaaggaga cgaaaacaat gcttatactt ttagtggttg gtctccggca
    11521 gttaagaaag ctacagaaga tgcaacttat atcgcacagt ttacagctag agaatctaaa
    11581 tatacagtaa cttggaagaa ttatgatggt acagttttag aagttgacga agatgttcca
    11641 tatgacacta tgcctgaata taatggtgct actccaacaa aagaaggaca tacttttatt
    11701 ggttgggaac ctgctttaag taaagtaaaa gttaatatag aatatactgc taaatttaaa
    11761 gttagtggag gaacaggtga acctacatca acagaagatt ttgtttttgc ggatgttgga
    11821 gaaggttatg ctttagttca ttattttggt aatgcaagcg atgttgtaat acctggtgaa
    11881 cataaaggaa aacctataat ttctattcaa aaatgggcat tttttgcaaa tgacacaatg
    11941 aaatcgctag ttattccaga tagtgttgtt gatattaaag aagatgcttt tgcacaatgt
    12001 aagattttag aaaggattac ctttggtagc ggtctaaaaa caatcggaat gatggctttt
    12061 gacagttgtt tggcactaga agctgttaat ttacctgaag ggtttgttca ccttgatata
    12121 atggtgtttt ctaattgtcc agcactaaca tcagttagtt taccaaacag cattgaaagt
    12181 tttggtgaat atatgtttga ttcatatgaa gagccaagta ccgtgacata taatgaatac
    12241 gcaaatggaa gatatttagg taatgctaca aaccctcatc tagttttagt agatatgatt
    12301 gataaagaag caacctcatt tactattcat aataattgta aacttataag cgatggtgct
    12361 ttatctaaaa gcaatatcga gacaattaat tttggtagcg gagtaaaaat aataggagga
    12421 aatgctttta aaaattgtaa gggattaaca gaattaataa ttcctgaagg tattataaag
    12481 attggcatta gtgcttttag tcaatcatct gttactaaag ttacaatagc agaaagtgtt
    12541 aaatatattg gcctttatgc ttttgaaagt tgttctcaat taacaacagt ttcgctacct
    12601 aatggtcttg aatttattgg gccttatgca tttgattata acgataattt aataacaaca
    12661 gactatgatg gttgcaatta tttaggtaat agcgacaacc ctcatttatt gttattagag
    12721 gcaagagata aatcgaaaaa caatattgaa attcataaag atacaaaaat gattgctgat
    12781 gtagcttttg ctcgttgtcg aaatttaaca acaattacaa ttcctaaaaa tgttaaattc
    12841 ttgggtaaaa atcttttcta tgatagtaac aatttaactg aagttattgt ggaagaaggt
    12901 aatatagtat atgaagctcc aaacaacaca gtaattattg aaaaagcaac aaaaacagtt
    12961 gttttaggcc ttaacaatgc ggttatacca gaaggagtaa caaaaatagg ggatgatgct
    13021 tttaataatt gtagccagtt agaaacaata ataattccag aaggagttac atcaattgga
    13081 gatagtgctt tttcttattg tatgaaatta gaatctgtga caattcctaa cagtgtaact
    13141 catataggaa gaagtgcttt ttctgattgt tataaattag gatcacttac aattccaaat
    13201 aaacttaaat atattggtca ctgggctttt catcgttgtc attcattaac aactcttgtt
    13261 gttccagatt cggtttctta tattgatgag tatgcttttg gtaattgtga gggattagaa
    13321 aatcttactt taggatctag tttagttgtt attggtgaaa ctgcattttc tggttgtgag
    13381 gttttacaat caattacaat tcctgatagt gtaaagacca tagagagcag tgctttttct
    13441 ggttgttccg ctgtaacatc aattaaaatt ggtaaaggag ttataagtat taacccttat
    13501 gcttttcttg gttgtaaagg agttgcaaca gcaactgttg atagtgaaaa taaagtttac
    13561 gattcaagag aaaactgtaa tgcaattatc gaaacagaac caaatattct tgtttttgga
    13621 actaaagcaa cgataattcc agatagtgtt acagaaattg gaagatatgc attttatgaa
    13681 tgtgtaaatc ttgaaaatct tgaattaagc gataacataa cagcaattgg tgaatatgct
    13741 ttttatcgct gttcaggctt aaatgcgata gttattccag aaagtgttgt aaatgttggt
    13801 cgttatgctt ttagaaggtg tacaaattta aagatttttg tagaaacaac agaattacca
    13861 gagacttggg atagcaattg gaatgaagga actccagttt attggtatag tgaggctcct
    13921 aattataatg gatcacattg gcattatgta gatggtgttc ctgtaatttg ggtccaacaa
    13981 taaataaaat tataaaacta aatacatatt taaaatatta atagatatat aaatatcgaa
    14041 aaaatataac tttacacgtc attaaacgca ggaggtttta tgaaaaaaat aagcgggatt
    14101 ttagttatgc tatttttagc agttatcttt ctttcaagtt gtagttgtgg tgaggcagaa
    14161 tattacacag ttacttggaa gaatcgcgac ggaacagttc tagaaattga cgaaaatgtt
    14221 gaagaaggaa catatccggt atatgacgga gaggaaccaa caaaagaaaa cgaagaacat
    14281 tatacttata gttttaatgg atggtctcca gcagttacgt atgttgaaga ggatgttact
    14341 tatattgcac aatttaaaaa aacacctatt gaatatacaa ttacttggaa aaactgggat
    14401 ggtacagttt tagaaacaaa tgaacaagct gcatatgata gcattcccca atataaaggc
    14461 gcaactccaa caaaagaagg atatactttt gatggttggg aacctgctat aagtaaagtt
    14521 gtaggaaatg caacatatac agctaagttt aaagttaaag aagatggtgg tggcggaggc
    14581 ggaggcgacg aaggaggaac agaatcatta gaaacagaat ttgtatttgc agatctaggt
    14641 gatggttata ctgttgttca ttatttaggt aatgccaata aagtttcaat tcctgagtat
    14701 tatatggaaa aacccgttat ctctattgac cgatgggcat ttaatagcaa caaaacaatg
    14761 acaaagttaa ttgttcctaa tagcattcaa gaaattaaaa aagatgctat ttgcaattgc
    14821 tctgctttaa aaacagttac tttaggtaat ggtataaaaa caataggtgc aaatgctttt
    14881 ggtatttgtc cgttattaga gactattaat ttacctgaag gccttactta tattggcgac
    14941 gaagcatttc gcgagtgtcc taaattaaga tcaatgagtt taccaaatag tattgagagt
    15001 tttggtaaag caatgtttaa gggctatgac aatatggaat acactacata tgaaggtggg
    15061 aaatatctag gtaataatgg aaatccttat ttagttcttg tgggaatgtc ttcttcttgg
    15121 aatacttcaa ttacaattca taataaatgt aaacttattg ctaatggcgc ttttgaaaag
    15181 ataaacttta cagaattaaa ctttggtact ggagttaaaa taataggcaa acttgctttt
    15241 tctcaatgca aaggactaac agaaataaca tttcctgata gtttgataaa aatagatgat
    15301 aatgctttta gtcagtcatc aataactaaa gttacatttg gaaataatct tagatacata
    15361 ggaaacaatg cttttgaaag aagtcgttct ttagtgtctg tttcaattcc taatagcatt
    15421 gaatatatgg gtatctatgc tttctatcaa cacgacaact tggcaactac cgaatataat
    15481 aatgcaaagt atttgggtaa tggtgaaaat ccttatttag tattattgaa agcaaagaac
    15541 acttcaataa ataatattga aattcacgaa aatacaaaaa taatagctgg ttttgctttt
    15601 gaaggatgtt ctagtttaac aacaattaca attcctaaaa atgttaagta tattggacgt
    15661 agcgcttttc aggcatgtaa tggattaaat aggattattg ttgatgaaga caacacagta
    15721 tatgcagcac ctaatgaatc agcaattatt gaaaaattaa cgtctcgtct tgcttttggg
    15781 tttaatgaaa cgattattcc aaacggagta gaaattattg gagcacatgc tttttataat
    15841 aatacacaaa taacagcaat tactattcca gaaggtgtta aaacgataga agattctgct
    15901 tttcttgctt gcacgaattt agaacgcgtt acattagcta atagtataac ttatattggt
    15961 aattcggctt ttgctgagtg tttaaatcta ggagcaatta gacttccaaa caatcttaaa
    16021 gttattgaag agagtgcatt ttatcgcaat cgtttggtaa cagaagttgt aattccgaat
    16081 actactacgt atattggcga caatgctttc tattattgcg gcacgcttag aaatcttact
    16141 ttaggaaata gtgtggttgt tatcggtaaa gatgcgttta aaggatgtag cacattgttt
    16201 catattgtaa taccagataa tgttcaaagt attaaatatg atgcttttgg tgaatgtaca
    16261 ggcacactta cgattacaat cggtagtgga gtaagattta tgcaaaaacc aggttttagt
    16321 gcttgtaata atgctacagc tcttacagta gatagtgaaa atcaagtttt tgattctaga
    16381 gaaaattgta atgcaattat tgaaacggca acaaatactc ttctttatgg aattaaaatt
    16441 tcaacaatcc cagctgatat tgaagctatt ggtgatgaag cttttaatgg ctctcaaata
    16501 acaacattag aacttagtgg taatgtaaaa tcaattggca gaaacgcatt ttataacact
    16561 tggttaactt cagttacatt agaagaagga cttgtttcaa ttggcgaata tgcttttggt
    16621 agatgtttat ggttaggtca aattgttatt ccaagaagcg ttacaacaat tgatcactat
    16681 gcttttgaca gcagcacatc tttaaatatt tatgcgaaag caacagataa acctgcaggt
    16741 tgggaatatt ggtggaattc aagtaatctt cctgtttatt ggtatagcga gacgcctaat
    16801 tatgacggaa accattggcg ttatgtcact ggtgctccaa caatttgggt ttcagaataa
    16861 aaacagatcc tacgaaaagc attgttttta aaattgatat tacacaaatg atattccaat
    16921 aatttgggaa taattgttta taaacattaa agacataaac tgatataatt agctttaaaa
    16981 gctataaaat agggcaatag atatcaaaaa atagtctatt gctcttttaa ttttgtgtgg
    17041 ttttttcttt caataattgg aataaagttg ttgactttta aattcaaata ttatataata
    17101 ataaagcacg tgatgagaat acgtgtctaa ataagtcttt
//
LOCUS       DAOSGS010000006        16913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_2758_length_16913_cov_5.046981, whole genome shotgun sequence.
ACCESSION   DAOSGS010000006 DAOSGS010000000
VERSION     DAOSGS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_16913_cov_5.046981"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLQ22_00665"
     CDS             <1..744
                     /locus_tag="PLQ22_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH58505.1"
                     /translation="EVTNLSFKYPGLEENVIENISFKIKAGESVGILGKTGSGKTTLI
                     DTLMRIYNVEDGMIKYDGIDINKIPLKVLRDNTAIVPQDNFLFYDTVYENIAFGTKRE
                     VTNHEVMEVAKLADLDSNIEEFQNGYETMIGERGSTISGGQKQRVSIARALIKEAPIL
                     ILDDSVSAVDTDTEDKIIRNLKTTRKGKTTILIAHRVSTVRKLDRILLIDEGKVIGFG
                     THEELLDNPLYSNLVRLQSLEEEIEGGGQ"
     gene            744..2525
                     /locus_tag="PLQ22_00670"
     CDS             744..2525
                     /locus_tag="PLQ22_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58506.1"
                     /translation="MPKGVKKISDARLFSKLLAYGKKYIFHYIIIFLVMVLAIGASLL
                     TDYLIGDVIDRLNGETPVEMASFARIILIFLGIIIATSILEYFSAIMLQKIGQGVIYN
                     VRGDVFNAIEHYSTSMLKETPAGKLATRVTTDVNTLSEMYTSVITNLLKNVLTIVGVL
                     VFMLVMNWRLALYTMICIPIVFVLSLLFRNFSRKIYRQVRSGVSEINSFVNENVSGMK
                     ITQSFNQEDRQMAQFEDKNIFLKKAHLKLIYGFALYRPSIYVITMLTTCLIIWVGGLD
                     VISFAGGFTVGALFTFIQLANKMFGPLQELAEQYNILQSAFASSERIFEAMGKKSEIE
                     EVPHPVVLEEIKGEIEFRNVWFKYPTSDWILQDVSFHINPKETVAFVGATGSGKTTIL
                     ALITRNYDIQKGEILIDGIDIRQIELNSLRKSIGQMMQDVFLFSGTVKTNINLRDDSI
                     SDEKVHEASVYVNADQFIQKLPNKYDEEVREHGNNFSSGQRQLLSFARTIAHDPKILI
                     LDEATANIDTETELLIQNSLEKMMSIGTMLIVAHRLSTIQHADKIMVISKGRLIEQGN
                     HQELLKQKGHYYKLYQLQYEVRGEENG"
     gene            2518..2988
                     /locus_tag="PLQ22_00675"
     CDS             2518..2988
                     /locus_tag="PLQ22_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58507.1"
                     /translation="MDRELIPALIIAAANDLDLSLPEINVSKMPDEIRAYTQFPHKVL
                     NFNQKWLKEANHIEMAGVVFHEMRHLWQFKQILLGDENKEGLEISEEEYELWKEELKK
                     KEDGEFSLDAILAIEVDAYAYANLQLEKLLVPLEEFNNEELKKLVSLRMETIRK"
     gene            3089..4303
                     /locus_tag="PLQ22_00680"
     CDS             3089..4303
                     /locus_tag="PLQ22_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH58508.1"
                     /translation="METKKRLDLKNLIGIIVVSVIVITTFALSLWYFKSTKEQEEKQY
                     YVYTIDLGNSFQTVSKVQIVSQDLSEEERQRLGEEMRAILEELDLEFNAVERIGGEKS
                     TLMKVNEKAGIEPVSVSGEFIYVLEKALEVASMSKVDGVALFDPTIAPVWQAWDFPEL
                     IFTTYEGPVPVEYLPTREELESLLPLVNYEKVVLDKTNNKVFLTEVGMALDLGSIVKG
                     YAADKIKEYLVSQNIDRAIIDVGANILLLGNYVNTKGEDIDWPLYVRTPSKNYLAGDL
                     NKLGKVLDNKDKTVVTSGDYEKYIIDEDGNHYHHILDPRTGYPIANGLVSVSIITDLS
                     ILGDAYSTTVFALGLEKGMEFIETTDNTEAVFVLKEGKNYKVYVSSGLENKFEFNASL
                     EEKNYQYQGVYR"
     gene            4294..5124
                     /gene="sufC"
                     /locus_tag="PLQ22_00685"
     CDS             4294..5124
                     /gene="sufC"
                     /locus_tag="PLQ22_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOH58509.1"
                     /translation="MSMKVLEIKDLHVSVENKEILKGINLSLKSGEFHAIMGPNGAGK
                     STLAAAIMGHFNYKVTKGSITLNGEDVLKMSVDERARKGLFFGMQYPSEVGGLTNAEF
                     IKKALEARLEEGKRLSLYKFIKELDQTVEELQMNKDLPERYLNEGFSGGEKKRNEILQ
                     MKLLKPNIALLDEIDSGLDVDGLKVVGEAINKMKSPEFGAMLITHYERLLDYLDIDQA
                     HVLVGGRIVKSGGIELIKKINKEGYDWVKTELGIFEEEEAPISLESCAVKESAARPKK
                     "
     gene            5121..5888
                     /locus_tag="PLQ22_00690"
     CDS             5121..5888
                     /locus_tag="PLQ22_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOH58510.1"
                     /translation="MRVLEHKKTIDSSGVYDFVFQSQTKNVTIDVKENINVTLHLIFK
                     EANNFNLKLKFKNNEVKLVNVCLDGASRVKQIFDASKSNIKALSCFNSKCDSFNLEAN
                     LKEESNIELDNIIINKENMKQVYDFRINHLSPYTESNLRNYAVTDNDSKLVINNTGFI
                     DFDCKKAAINQSTKGIMLDLYSSIEANPILEINHHDVIANHGASIGALADSDLFYLMS
                     RGIPKEEAERIIIEGYYRTILDKIEDKDLKQEIEKRL"
     gene            5889..7100
                     /locus_tag="PLQ22_00695"
     CDS             5889..7100
                     /locus_tag="PLQ22_00695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOH58511.1"
                     /translation="MLDAKKTRKDFPIYQNNKDMVYLDNAATVLKPKKVLDKMNEYYT
                     HFGVNIHRGVYKLSYLATKAYEEGRQKVADFINASFEEIVYFKNASEALNFVALSWGE
                     TNIKEGDEIITSELEHHSSVLPWQQLALRKKAVLRYLKLDSKGRITIDALKSVLNEKT
                     KVIALTLVSNVFGYITPIEELIKEARKYNSLVVVDAAQGIAHLPIDVKKMDCDFLAFS
                     AHKALGPTGLGVLYGKKDIFKKLKPLFYGGDMIDNVELYDSSYREMPYRFEVGTPAIA
                     EIIGLGYALDYLKDIGLDNIKKHEQELTKYALEKMKVLKHLTIYNSNPDLGIIAFNLD
                     GVHPHDVATILDSDNVYVRAGHHCAQLIAKKLEIVASLRVSFFIYNDKEDIDKLIEAL
                     KKAINFFYQGE"
     gene            7102..7539
                     /locus_tag="PLQ22_00700"
     CDS             7102..7539
                     /locus_tag="PLQ22_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOH58512.1"
                     /translation="MDKLRNLYQTVIMDHYRNPYNKGLKGYPKVHLKNPACGDDITIE
                     AKVNNGVIETINHNGTGCSICCASASMLCLLMEGKTVEFARKEINKFYNLVQGKDGDY
                     ESLGDANSLSGVASFPARIRCATISWKALEMVLEGDLDEEREI"
     gene            7520..8920
                     /gene="sufB"
                     /locus_tag="PLQ22_00705"
     CDS             7520..8920
                     /gene="sufB"
                     /locus_tag="PLQ22_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH58513.1"
                     /translation="MKKEKYDIVGDYKYGFKTETDAVFKTKKGLNREVIETISKEKNE
                     PKWIRDFRMKSYEEFLVHKYPSFGPNLEFIDFDDYIYYIKATDQVEKSWDDVPDEIKD
                     TFEKLGIPEAEREYLAGVTTQFESEAVYHNQLKELEELGVIFTDTDTAVRKYPNLVKQ
                     YLGTVVPYNDNKFAALNSAVWSGGTFIYVPKGVKIDKPLQSYFRINTERMGQFERTLI
                     VVDEGASLHYVEGCTAPIYSSDSLHAAVVEIVVKENGYCRYTTIQNWSTNILNLVTKR
                     AQVEANGHMDWIDGNIGSGLNMKYPACILKGENAKGSCISIAFANKGQYQDAGAKMIH
                     LAPNTSSTIISKSLCRGGGQVNYRGTVYHDPKALNARSFIECDTIILDDQSISDTIPF
                     NIACNQKSRIEHEATVSKISDEQLFYLMSRGIPEEEAMEMIVMGFIEPFSKELPMEYA
                     VELNQLIKLEMTDSIG"
     gene            8924..11206
                     /locus_tag="PLQ22_00710"
     CDS             8924..11206
                     /locus_tag="PLQ22_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOH58514.1"
                     /translation="MKKTILLFLLLIPFILTGCFCDKSKEHSFTLIILNEQEQIINEK
                     VAFNEGENLLEILKNKYGLVYNQEQGKILQIKNIELAETGYFTITLNDVEQTSFVFEM
                     KDQYILKIQYHKESSFKEYEVTFKEVDDTLISSYVWPNNSLVSKPAPPEKEGYTFKGY
                     YLDKEATLEFDFNSYLTRDTVIYFIYEEIVSEVSYTIEFPTQALRKKTAGEDETIGIV
                     TYEPSSASIKTMSVTSSDDTKLSCTLYLDQVDNVYKVKAKCLDFGTAKVIVNLNGKIQ
                     EYEFTLYPRREEDVPDNEWFEMSFEEQEAMRLDRERREAEVIDNEVERIKTWFTENIP
                     DSTVGNFDLIERSDVSGVYVNWHSDNKNVLYLTYVDNVYVGQITQISYDTNATLSLTV
                     STASSSETITKEIAVPGFNLIPLPDHPLVFAYVPIWNFTGISPEDISRIDVFHLCFGY
                     VKNGEFDVSHIEFLMPHIMPLRRMGKRVVLSVHDGGKLASATDDGVEILGFSDAVSTA
                     EKRTRTVNQIMETVIKYDLDGVDLDWEGPGFSYSSNPCHPDDVINFYLFVKELKAAMD
                     ASPRADLLLTAACAAPKADQYSLDKLKDDFDYIHIMTYDFVIFTTTSHNSALLEGPGS
                     TYFSTEKGLNEYLRYMPREKLTVGAAFYGKIYNLPNGIGDGLSQTVSEACKTINYDKI
                     RQEYWTDLNDETYIHKSEVTKATWYYDGSSWITFESPWAVAEKAKWAKNQNLGGIMFW
                     DYNTDYQNELMSALYYNFRY"
     gene            11227..13509
                     /locus_tag="PLQ22_00715"
     CDS             11227..13509
                     /locus_tag="PLQ22_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOH58515.1"
                     /translation="MKKTILLFLLLIPFLLVGCSCEKKPEDQEFTFTFELYDEKSLIV
                     KETITYKEGDNLLEILKSKYNLVYSEGIQKINNLALSEKNYFLITLNGEEKTDFSFML
                     KEGDLLKIEYLEDEIIEVNYNVSFREVDETEILALEVKENDKITAPTVANKDGYNFKG
                     FYLDKEGVTLFDFASNVTSNLVIYLVYEEISQIETIDINVEFPTLSLRKMTPPENKTN
                     GLIVLEPADAKIETYSLRVSDTTKLRSFIFTDPQDGLRKVHVECLDYGEAEVTITING
                     ITKTYEFTLYPRREEDVPFEEWVEMSFEEQEAMRLDRERREREAEVNEVTRLKTWFEE
                     HIPANTTESFELINGTNVESVYIMWHSNHKGVIYPKVVDGVYTAQVTQITYDVVVNLT
                     LTINSSSGSEELTKAITIPKGFDLRPLPQRPLIFAYVPTWGYEGIRQEDVAKIDVFNL
                     CFAYVTNGMFTVSTIEALLPTIMPLREVGKRVVMSVQDGGKNGPPLIPGFSAAVSTAA
                     GRTTIVNQMVQAVIEYDFDGIDLDWEGPGFSFSSNPSSPNDIQNFYLFVKELRAAMDD
                     SPRKDLLLTAACAMSYPNNYSLDRLQNEFDYIHIMTYDGGSSTTTSHCSALLPGPGAA
                     WSAQRGLNNFIATGFPRNKLTIGSAFYGKRYLNVSSTGDGLGVSVAQAASTMTYKNLR
                     KEYWTNLSDETYIYTSQETKTSWYYNKDTGVWIGFESPWAVAEKAKWAKAQGLGGIMF
                     WEYNQDYNGELLAAMYNNFR"
     gene            13603..14685
                     /gene="gcvT"
                     /locus_tag="PLQ22_00720"
     CDS             13603..14685
                     /gene="gcvT"
                     /locus_tag="PLQ22_00720"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOH58516.1"
                     /translation="MKKTVLYDEHIKQSGKMIEFTGYLLPVYYTSISEEHELVRSDFG
                     CFDCSHMGEIMISGEDAYKFTDYLVTQNLSGPDLRMSYGFLLNENGGVVDDIMVYKYN
                     DSKFLLVVNASNKEKDYEHIMKLFKKGKYNCDVVDMSDDISLLAIQGPNAASFLQKDL
                     VDKLDELKMYDFKYYSILGMKFLISRSGYTGGDGFEIYGKNEDILKLYQHLTKRGMKS
                     CGLGARDTLRFEAALPLYGQELDMDINPLEAGLSFGVDFNKDFYGKEALSKIKEEGLK
                     RRSVGLELLGPGIARHGYKVYSEGKEIGYVTTGYMIPNTKDSYAVALIDRDEYKLDKE
                     VEILVRKKFVKAKIIKKKFMDKKYQK"
     gene            14695..15075
                     /gene="gcvH"
                     /locus_tag="PLQ22_00725"
     CDS             14695..15075
                     /gene="gcvH"
                     /locus_tag="PLQ22_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH58517.1"
                     /translation="MADIKKGLFYTKTHEWVKVEGNVAYIGLTAFAASELGDIIYFEG
                     PDADDVAQGAECGVVESVKSASDIYSPVSGKVLEVNEAVLDSPELINEDPYGAWLVKV
                     ELDSKEELKKLLSAEAYEKELANA"
     gene            15068..16378
                     /gene="gcvPA"
                     /locus_tag="PLQ22_00730"
     CDS             15068..16378
                     /gene="gcvPA"
                     /locus_tag="PLQ22_00730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOH58518.1"
                     /translation="MHKYLPHTASDIKEMLDKIGIKTIDELFVALGDIKLTKPLDIPS
                     KMSENELRDHFGRLASKNKNLLSFCGLGSYESYDFSIIDALISRQEFLTSYTPYQPEV
                     SQGTLQYIFEFQSYVCELTGMDIANASMYDGASSTAEAIFMAVAATRKNKVLLSKTIN
                     PYVLEVVKTYSKYRDLEVEYVLEKDFSFDLEDLKKKLNNDVACFVAQTPNVYGVLEDY
                     SKVKATLEEIGALFIINQEPSSLALFKAPSEYNADIACGDMQSLGLKRNFGGPSVGYI
                     ATISKYQRRLPGRIVGKTVDVEGKRGYVLTLQAREQHIRREKATSNICSNQSLMALTV
                     SIYLSLVGKLGLIELSKRAYNNAHYLEEELLKTKKFKKVTSRPFYQEFVLEYLGNPKH
                     LEQKLLALGYLSGYNIKDNLIIFYASEVRTKDEIDGFVKKVGEV"
     gene            16378..>16913
                     /locus_tag="PLQ22_00735"
     CDS             16378..>16913
                     /locus_tag="PLQ22_00735"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157953.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOH58519.1"
                     /translation="MYDKLIFERSKKGRVGITLPKTDIKDYALPKEHLTKRDLLLPEV
                     SEVDVIRHYTNLSLKNYGVDRGIYPLGSCTMKYNPKINEELSKNPKFNNLHPLQPDVS
                     SQGALELEYNLEKLLNEILGLKAFSFAPCAGAHGELTGLMMIKAYHEKNKDFKRKKII
                     IPDSAHGTNPASVAIVGF"
BASE COUNT         6234 a         1943 c         3220 g         5516 t
ORIGIN      
        1 gaagttacta atttatcgtt taaatatcct ggtttagaag aaaatgttat tgaaaatatt
       61 agttttaaaa tcaaggctgg cgaaagtgtt ggaatcttag gtaaaactgg atctggtaaa
      121 actactttaa ttgatacatt aatgagaata tataatgtag aagacggaat gattaaatat
      181 gatggcatag atatcaataa aattccatta aaagttttaa gggacaatac tgctattgta
      241 ccacaagata atttcttgtt ttatgatact gtttatgaaa atattgcttt tggaacaaaa
      301 agagaagtta ctaatcatga agttatggaa gttgctaaac tagcagactt agatagtaat
      361 attgaagagt tccaaaatgg gtatgaaaca atgataggtg agcgtggttc aacaatctct
      421 ggtggtcaaa agcaaagagt gtcgattgct agagctttaa ttaaagaagc accaattttg
      481 attttagacg attctgtttc agctgtagat actgatactg aagataaaat tattagaaac
      541 ttaaaaacaa caagaaaagg aaaaacaacg attttaatag cacaccgtgt atctacagta
      601 agaaaacttg atcgtatctt actaatagac gaaggtaaag ttattggttt tggaactcat
      661 gaagagttac tagataatcc tctttatagt aatctagtga gattacaatc tcttgaagaa
      721 gaaattgaag ggggtggaca ataatgccta aaggtgtaaa gaaaataagc gatgcaaggc
      781 tttttagtaa actgctagct tatggtaaaa aatatatttt ccattacata attatctttt
      841 tagtaatggt acttgctatt ggtgcatcac tattaaccga ttatttaata ggagatgtaa
      901 ttgaccgttt aaatggtgaa acgccagttg agatggctag ttttgcgaga attattttaa
      961 tatttttagg aattattatt gcaacttcta ttttagaata tttctctgca atcatgctgc
     1021 aaaaaatagg acagggcgtt atttataatg taagaggaga tgtatttaac gcaattgaac
     1081 attactctac aagtatgctt aaggagactc cagctggtaa attagctaca agagttacaa
     1141 ctgatgtaaa tactttaagt gaaatgtata cgtcagtaat tactaacctt ttgaaaaatg
     1201 tacttacaat cgttggtgta ttagttttta tgcttgttat gaattggcga ttagctttat
     1261 atacaatgat ttgtattcca attgtttttg ttttatcatt attgttccgt aatttctctc
     1321 gtaagattta tcgccaagta agatctggag taagtgaaat taactcattt gtaaatgaaa
     1381 atgttagtgg aatgaaaatt acacaaagct ttaatcaaga agataggcaa atggctcaat
     1441 ttgaagataa aaatatcttc ttaaagaaag cgcacttaaa attaatttat ggttttgcat
     1501 tatacaggcc atcaatttat gtaattacga tgctaacaac ttgtttaatt atttgggttg
     1561 gtggattaga tgttattagt tttgctggtg ggtttactgt aggtgcttta tttacattta
     1621 ttcaattagc gaataagatg tttggaccat tacaagaact tgcagagcaa tataatatct
     1681 tacaatctgc gtttgcttca agtgagagaa tttttgaagc aatgggcaaa aagtcagaaa
     1741 ttgaagaagt gccgcatcca gtagttttag aagaaattaa aggtgaaatt gagtttagaa
     1801 atgtttggtt taaataccca acatctgatt ggatcttaca agatgtaagt ttccatatta
     1861 atccaaaaga aactgttgct tttgtagggg caactggatc agggaaaaca acaattttag
     1921 ctttgattac aagaaactat gatattcaaa aaggtgaaat tttaattgat ggtattgata
     1981 ttagacagat tgaactaaat agtttaagaa aaagtattgg ccaaatgatg caagatgtat
     2041 tcttatttag tggaacagtt aaaaccaaca ttaatttaag agatgattca attagtgatg
     2101 aaaaagtaca tgaagctagt gtttatgtaa atgctgatca atttattcaa aagcttccaa
     2161 acaaatatga tgaggaagtt agagaacacg gaaataactt ttcttcagga caaagacaat
     2221 tactatcatt tgcaagaact atagctcatg atccaaagat tttaattttg gacgaagcaa
     2281 cagctaatat agatacagaa actgaacttc taatccaaaa ctctttagag aaaatgatga
     2341 gtattggtac aatgttaatt gtagctcata gattatctac aattcaacac gcagataaga
     2401 ttatggttat tagtaagggt agattaatcg aacaaggtaa tcatcaagaa ttactaaaac
     2461 aaaaaggaca ttattataaa ctatatcaat tgcaatatga agtaagaggc gaggaaaatg
     2521 gatagagagt tgatacccgc tttaatcatc gcagctgcaa atgacttaga tttaagtttg
     2581 ccagaaataa acgtttctaa aatgccggat gaaattagag cttatactca atttcctcat
     2641 aaagttctta acttcaatca aaagtggctt aaagaagcaa accatataga gatggcagga
     2701 gtagtttttc atgaaatgag gcacttatgg caattcaaac aaattttgct tggcgatgaa
     2761 aacaaagaag gtttagaaat aagtgaagaa gaatatgaac tttggaaaga ggaattaaag
     2821 aaaaaagaag atggtgaatt ttctttagat gctattctcg caattgaagt agatgcctat
     2881 gcttatgcta atttacagct tgaaaaatta cttgttcctt tagaggaatt taataatgaa
     2941 gagttgaaaa aattagttag tttaaggatg gaaacaatta gaaaataaaa taaaagcatg
     3001 atgacaatta gtgttatcgt gcttttttta ctatataatt tgcatttttc gcattaaatg
     3061 ctataataaa tattataatg gagtaattat ggaaactaaa aaaagactag atttaaaaaa
     3121 cttaattgga ataatagttg ttagtgtaat agttatcaca acatttgctt taagtttatg
     3181 gtattttaaa tcaacaaaag aacaagaaga aaagcaatat tatgtttata caattgactt
     3241 aggaaattca tttcaaactg taagtaaagt tcaaattgta agtcaagatt tgagtgaaga
     3301 agaaagacaa agactaggag aagagatgag agctattcta gaagaacttg atttagaatt
     3361 taatgcagtt gaaagaatcg gtggagaaaa atcaacttta atgaaagtaa acgaaaaagc
     3421 tggaattgaa cctgtttcag tatctggtga atttatttat gtattagaaa aagcactaga
     3481 agtagctagt atgtcaaaag ttgatggtgt tgctttattt gacccaacaa ttgcccctgt
     3541 atggcaagct tgggattttc ctgagttaat ctttacaact tatgaaggtc ctgttcctgt
     3601 tgaatacctt ccaacaagag aagaacttga aagtttgctt cctttagtta attacgaaaa
     3661 agttgtttta gataaaacaa acaacaaagt tttcttaaca gaagtaggaa tggctttaga
     3721 tttaggttcg attgtgaagg gatatgcagc tgataaaatt aaagaatatt tagttagtca
     3781 aaacattgat agagctatta ttgatgttgg agctaatatt ttacttttag gaaattatgt
     3841 gaatactaaa ggcgaagata ttgattggcc tttatatgta agaactccta gcaaaaatta
     3901 tttggcgggt gatttaaata aattaggtaa agtcctagac aataaagata aaacagtagt
     3961 tacaagtggt gactatgaaa aatatattat cgatgaagac ggtaatcatt atcatcacat
     4021 tttagaccca agaacaggtt atcctatagc taatggttta gtatcagtat caattattac
     4081 tgacctttca attttaggag atgcttatag tacgactgtt tttgctttag gtttagaaaa
     4141 gggtatggaa tttattgaaa caacagacaa caccgaagct gtgtttgttt tgaaagaagg
     4201 aaaaaactat aaagtctatg tttctagtgg attagaaaac aagtttgaat ttaatgcgtc
     4261 actagaagaa aagaattatc aatatcaagg ggtatatcga tgaaagtatt agagattaaa
     4321 gatttacatg taagtgtaga aaataaagag attttaaaag gaattaactt aagtttaaag
     4381 tcaggagaat ttcatgcaat tatgggtcct aatggagcag gaaagtcaac gctagctgct
     4441 gctattatgg gacactttaa ttataaagtt acaaaagggt cgataacttt aaatggtgaa
     4501 gatgttttaa aaatgagtgt tgacgaaaga gcaagaaaag gcttgttttt tggaatgcaa
     4561 tatcctagtg aagtaggtgg ccttactaac gcagagttta ttaaaaaagc tttagaagca
     4621 agacttgaag aaggtaaaag attatcacta tataagttta ttaaagagtt agatcaaacc
     4681 gttgaagagt tgcaaatgaa caaagatcta ccagaaagat atctaaacga aggattttct
     4741 ggtggtgaga aaaaacgtaa tgagattttg caaatgaaat tattaaaacc taatattgct
     4801 ttacttgatg aaatagactc tggattagac gtagatggtt taaaggttgt gggtgaggca
     4861 attaataaga tgaaaagtcc tgaatttggt gcgatgctta ttactcacta tgaaagacta
     4921 ttagactatt tagatattga tcaagcccat gttttagttg gaggaagaat cgttaagtct
     4981 ggtggaatag agttaattaa gaaaattaat aaagaaggtt atgattgggt taaaacagaa
     5041 cttggaattt ttgaagagga agaagcacca atttcacttg aaagttgtgc agttaaagaa
     5101 tcagctgcaa ggccgaagaa atgagagttt tagaacataa aaaaacaatt gatagttcgg
     5161 gagtttatga ttttgttttt caaagccaaa caaaaaatgt aactattgat gtaaaggaaa
     5221 acattaatgt aacacttcac cttattttta aagaagcaaa taattttaat ttaaaattaa
     5281 aatttaaaaa taacgaagta aagttagtta atgtttgttt agatggtgca agtcgagtta
     5341 aacaaatttt tgatgctagt aaaagcaata ttaaagcttt aagttgtttt aattcaaaat
     5401 gtgatagttt taatttagaa gcaaatttaa aagaagaaag caatattgaa cttgataata
     5461 ttattatcaa taaagaaaat atgaaacaag tttatgattt tagaatcaat cacttgtctc
     5521 cttatacaga atcaaattta agaaactacg ctgttacaga caacgattct aaactagtta
     5581 tcaataatac agggtttatt gattttgatt gcaagaaagc tgcaatcaat caatcaacta
     5641 agggaataat gttagattta tattcttcaa ttgaagctaa tccaatttta gaaattaatc
     5701 atcatgatgt tattgcaaac catggtgcat caattggagc attagccgat agtgatttgt
     5761 tttacttaat gagcagagga atccctaagg aagaagctga aagaataatt attgaaggat
     5821 attatcgaac tattttagat aaaattgagg ataaggattt aaaacaagaa atagagaaga
     5881 ggttataagt gttagacgca aagaaaacaa ggaaagattt tccaatttac caaaacaata
     5941 aagatatggt ttatttagat aacgcagcta ctgtcttaaa acctaaaaaa gttttagata
     6001 agatgaatga atactatact cactttggag ttaatatcca ccgtggggta tataaactat
     6061 cttatctagc aactaaagcc tatgaagagg gaagacaaaa agttgcagat tttattaacg
     6121 ctagttttga agaaatagtg tattttaaaa atgcatctga agctttaaat tttgtagctt
     6181 taagttgggg cgagactaat ataaaagaag gggatgaaat tattacttct gagttagaac
     6241 atcactcttc tgttttgcca tggcaacagt tagcgttaag gaaaaaagcg gtcttaagat
     6301 atctaaaact tgattcaaaa ggtagaatta ctattgatgc tttaaagtca gttttaaatg
     6361 aaaaaactaa agttattgct ttaactttag tttctaatgt ttttggttat attacgccaa
     6421 tcgaagaatt aattaaagag gcaagaaaat ataactcttt agtagtagtt gatgctgctc
     6481 aaggtatcgc tcatttgccg attgatgtaa agaaaatgga ttgtgacttt cttgcatttt
     6541 ctgctcataa agctttaggg ccaactggtc ttggtgtttt gtatgggaaa aaagatatct
     6601 tcaaaaagtt aaaaccttta ttttatggtg gagatatgat tgataatgta gagttatatg
     6661 attctagtta tcgtgaaatg ccttatcgtt ttgaggtagg aacacctgcg atagccgaaa
     6721 ttattggtct gggttatgct ttagattatt taaaagatat aggattagat aatattaaaa
     6781 aacatgaaca agaattaact aagtatgctt tagaaaaaat gaaagtttta aagcatctaa
     6841 ctatttataa ttctaatcct gatttaggaa ttattgcttt taatttagat ggagttcatc
     6901 ctcatgatgt agcaactatt ttagattcag ataatgttta tgtgagagct ggacatcatt
     6961 gtgcacaatt aatagcaaaa aaattagaaa ttgtggcatc tttaagagta tcatttttta
     7021 tctataatga taaagaagat atcgataagt taattgaagc actaaagaaa gctataaact
     7081 ttttttacca aggagaataa aatggataaa ttacgtaatt tgtatcaaac tgtaatcatg
     7141 gatcattatc gtaatcctta taataaagga ttaaaaggat atccaaaagt tcatttaaaa
     7201 aatccagctt gtggggatga tattactatc gaagctaaag ttaataatgg agttattgaa
     7261 acaatcaatc ataatggaac tggttgttct atttgttgtg catcagcttc aatgctttgt
     7321 ttattaatgg aaggcaagac cgttgagttt gcaagaaaag aaataaacaa attttataat
     7381 ttagttcaag gaaaagatgg agattatgaa agtttaggcg atgctaatag tttatctggt
     7441 gttgcttcgt ttcctgccag aattcgttgt gcaacaattt cttggaaagc attagagatg
     7501 gtcttagaag gagacttaga tgaagaaaga gaaatatgat attgtagggg attataaata
     7561 tggttttaag acagaaactg atgctgtttt taaaactaaa aaaggcttaa atagagaagt
     7621 aattgaaaca atatctaaag aaaaaaatga accaaaatgg ataagagatt ttaggatgaa
     7681 atcttacgaa gagtttttag ttcacaaata tccttctttt ggcccaaatt tagaattcat
     7741 tgattttgat gattatattt attatataaa agctactgat caagtagaaa aaagttggga
     7801 tgatgttccc gatgaaatta aagacacttt tgagaagtta ggaattcctg aagcagaaag
     7861 agagtactta gcaggagtta caacgcagtt tgaatctgag gctgtttatc ataatcagtt
     7921 aaaagagtta gaagaactag gtgttatctt tacagataca gatacagctg ttaggaaata
     7981 tcctaatctt gtaaagcaat atttaggaac tgtagttcct tataatgaca ataaatttgc
     8041 agctttaaac tctgctgttt ggagtggggg aacatttatt tatgttccta aaggagttaa
     8101 aatagataaa ccattacaat cttattttag aattaatact gaaagaatgg gacaatttga
     8161 aagaacatta atagtggttg atgaaggtgc tagtcttcat tatgtagagg gttgtacagc
     8221 acctatttat tcaagtgatt ctttgcatgc agctgttgta gagattgttg taaaggagaa
     8281 tggatattgc cgttatacga ctattcaaaa ttggtctaca aacattctta atttagttac
     8341 taaaagagct caagttgaag ctaatggtca tatggactgg attgatggta atataggttc
     8401 aggattaaat atgaagtatc ctgcttgtat tttaaaagga gaaaatgcga aaggtagttg
     8461 tatttcaata gcctttgcaa acaaaggtca atatcaagat gcaggggcga agatgattca
     8521 tttagcgcct aatacatcaa gcacgattat tagtaaatca ctatgtcgtg gtggtgggca
     8581 agtaaattat cgtggcacag tttaccacga tccaaaagct ttaaatgctc gtagttttat
     8641 tgaatgcgat acaatcattt tagatgatca atctatcagt gatacgattc catttaatat
     8701 tgcatgtaat caaaaatcaa gaatagaaca tgaagccact gtttctaaaa ttagtgatga
     8761 gcaattgttt tatttaatga gtagaggaat tccagaagag gaagctatgg agatgatagt
     8821 aatgggtttt attgaaccat tctctaaaga actaccaatg gaatatgcag ttgaattaaa
     8881 tcaactaatt aaactagaaa tgacagattc gatagggtaa tttatgaaaa aaacaatttt
     8941 actattttta ttgctaattc catttatttt aactggatgt ttttgtgaca agtcaaaaga
     9001 acactctttt acattaatta ttcttaacga acaagaacaa ataattaatg agaaagttgc
     9061 atttaatgaa ggcgaaaatt tacttgaaat attgaaaaat aaatatggtt tagtttataa
     9121 tcaagaacaa ggaaaaatcc tacaaattaa aaacattgag ttagcagaaa ctggttattt
     9181 cacaataacc ctaaatgatg tagaacaaac tagttttgtt tttgagatga aagatcagta
     9241 tatattgaaa attcagtatc ataaagaatc atcattcaaa gaatatgaag ttacttttaa
     9301 ggaagttgat gatactttaa ttagttctta tgtttggcct aacaattcac tagtttcaaa
     9361 gccagctcca cctgaaaaag aaggatatac ttttaaaggt tattatttag ataaagaagc
     9421 gactttagaa tttgatttta attcttatct tactcgtgat acagtaattt actttattta
     9481 tgaagagata gtaagtgaag ttagttatac aatagagttt cctacacaag cattacgaaa
     9541 gaagactgcg ggtgaagatg aaactatagg tatcgttact tatgaaccta gtagtgctag
     9601 tattaaaacg atgtctgtta ctagtagtga tgatactaaa ttgagttgta cgctttattt
     9661 agatcaagta gacaatgttt ataaagtaaa agcaaaatgt ttagattttg gtacagcgaa
     9721 agttatagta aatttaaatg gtaaaattca agaatatgaa tttactttat atccaagaag
     9781 agaagaagat gtgccagata atgaatggtt tgaaatgtct ttcgaagaac aagaagcaat
     9841 gagacttgac agagaaagaa gagaagccga agttattgat aatgaagttg aaagaattaa
     9901 aacttggttt actgaaaata ttcctgatag tactgttggt aattttgatt tgattgaaag
     9961 atcagatgta tcaggtgttt atgtgaattg gcattctgat aataaaaatg ttctttactt
    10021 aacatatgta gataatgttt atgtaggtca aataacgcaa attagttatg atactaatgc
    10081 tacattatca ttaacggtta gtacagctag tagtagtgaa acaattacga aagaaattgc
    10141 tgttccagga tttaatttaa ttccgcttcc agatcatcca ttagtttttg cttatgtacc
    10201 aatttggaat tttacaggaa tttcacctga agatatttca agaatcgatg ttttccattt
    10261 atgttttggt tatgtgaaga atggcgagtt tgatgtatct catattgagt ttttaatgcc
    10321 gcacattatg cctttaagaa gaatgggtaa acgagtagtt ctttctgttc atgatggcgg
    10381 taaattagca tcagctactg atgatggagt agaaatacta ggatttagtg atgctgtttc
    10441 aacagctgag aaaagaacaa gaactgttaa tcaaataatg gaaacagtta ttaagtatga
    10501 tttagatggt gtagatttag actgggaagg acccggattt tcatattcta gtaatccgtg
    10561 tcatccagat gatgttatta atttctactt gtttgtgaag gaattaaaag cagcgatgga
    10621 tgcatcacct agagcagatt tattattaac tgctgcttgt gctgcgccta aagcagatca
    10681 atattcttta gataaactaa aagatgattt tgattatatt catattatga cttatgattt
    10741 tgttattttc acaacaacat cacataactc ggctttatta gaaggtcctg gttctactta
    10801 tttttctacg gaaaaaggat tgaatgaata tttaagatat atgccaagag aaaaactaac
    10861 agttggtgca gctttctatg gtaaaatata taatcttcct aatggcattg gagatggatt
    10921 aagtcaaacc gtaagtgaag cgtgtaaaac gattaattac gataaaatta gacaagaata
    10981 ttggactgac ttaaatgatg aaacatatat tcacaaatca gaagtgacta aggcaacttg
    11041 gtattatgat ggtagttctt ggattacttt tgaaagtcca tgggcagtag ctgagaaagc
    11101 taaatgggct aaaaatcaaa atttaggtgg aatcatgttt tgggattata atacagatta
    11161 tcaaaatgaa ttaatgtctg ctttatatta taattttaga tattagcgga ctagataagg
    11221 agacttatga aaaagacgat attattattt ttattactta tcccgtttct tcttgtagga
    11281 tgttcttgcg aaaagaagcc ggaagatcag gaatttacat ttacctttga actttatgat
    11341 gaaaaatcat taatagtaaa agaaactatt acttataaag aaggagataa tttattagag
    11401 attttaaaat ctaaatataa tttagtttat agtgaaggta ttcaaaagat taacaattta
    11461 gcattatcag aaaagaatta ttttctaata accttaaatg gtgaggagaa aactgatttt
    11521 agttttatgc ttaaagaagg ggatctttta aaaattgagt atttagaaga tgagattata
    11581 gaggtaaatt ataatgtttc atttagagaa gtagatgaga cagaaatttt agctcttgaa
    11641 gtaaaagaga atgacaaaat tactgctcct actgtagcaa ataaagacgg atacaatttt
    11701 aaaggttttt atttagataa agaaggagtt acattatttg attttgctag taatgttact
    11761 agtaatttag taatctattt agtttatgaa gagatttctc aaatagagac tattgatatt
    11821 aatgtagagt ttccaacttt aagcttaaga aagatgactc ctccagaaaa taaaaccaat
    11881 ggtttaattg ttttagagcc agcagatgcg aaaattgaaa cttatagttt aagagttagc
    11941 gatactacaa aattaagaag ttttattttt actgatcctc aagatggatt acgtaaagta
    12001 cacgtagagt gtttggatta tggtgaagca gaagtaacta ttacaataaa tggtattact
    12061 aaaacatatg aatttacttt atatccaaga agagaagaag atgtaccatt tgaggaatgg
    12121 gtagaaatgt catttgagga gcaagaagct atgagacttg atagagaaag aagagaaaga
    12181 gaagcagagg ttaatgaagt tacaagatta aaaacttggt ttgaggaaca tatacctgct
    12241 aatactactg aaagttttga attaattaat ggaacaaatg tagagagtgt ttatataatg
    12301 tggcattcta atcataaagg agttatttat cctaaagttg ttgatggtgt atatactgct
    12361 caagtaacac aaattactta tgatgttgtt gtgaatttaa cattaacaat taattcttct
    12421 tcaggtagtg aagaacttac aaaagctatt actattccaa aaggatttga tttaagacca
    12481 ttacctcaaa gaccattaat ttttgcttat gttcctactt ggggatatga aggaattagg
    12541 caagaagatg ttgcgaaaat tgatgtcttt aatttatgct ttgcttatgt aacaaatggt
    12601 atgttcactg tttccacaat cgaagcacta ctacctacaa ttatgccttt aagagaagtt
    12661 ggcaaaagag tagtgatgtc agttcaagat ggtggtaaaa atgggccacc acttattcct
    12721 ggatttagtg ctgctgtttc gacggctgca ggtagaacta caattgtaaa ccaaatggtt
    12781 caagcagtaa ttgaatatga ttttgatggt attgatttag attgggaagg accgggattt
    12841 tcattctcaa gtaatccttc tagtcctaat gatattcaaa acttctattt gtttgtaaag
    12901 gaattaagag cagcaatgga tgattcacca agaaaagatt tattattaac tgctgcttgt
    12961 gcaatgagtt atccaaataa ttattcttta gatagattgc aaaatgaatt tgattatatt
    13021 catattatga cttatgatgg tggatcttct actacaacat cacactgttc tgctttatta
    13081 ccaggacctg gagcagcatg gtctgcccaa agaggattaa ataattttat tgcaactggt
    13141 tttccaagaa ataaattaac tattggttca gctttttatg gtaaaagata tcttaatgta
    13201 tctagcacag gtgatggctt aggagttagc gttgctcaag ccgcttccac aatgacctac
    13261 aaaaacctaa gaaaagagta ttggacaaat ttaagtgatg aaacatatat ttacacttct
    13321 caagaaacta aaacatcttg gtattataat aaagatacag gcgtctggat tggatttgaa
    13381 agtccatggg cagtagctga aaaagctaaa tgggctaaag ctcaaggatt aggtggaata
    13441 atgttctggg agtataatca agattataat ggtgaattat tagcagctat gtataataat
    13501 tttagataaa aagtaaaagg caaaagatat tcttttgcct tttgttttgc ttgtttagtt
    13561 tttcaaataa tggtatacta atatcaataa aggtggatag ttatgaagaa aacagtattg
    13621 tatgatgaac acattaaaca atcaggaaag atgattgaat ttactggtta tttacttccc
    13681 gtatattaca catcaattag cgaagaacat gaacttgtta gaagtgattt tggctgtttt
    13741 gattgttcac atatgggaga gattatgatt tcgggagaag atgcttataa gtttactgat
    13801 tatttagtaa cacaaaattt aagtggacca gatttaagaa tgagttatgg ttttttatta
    13861 aatgagaatg gcggagttgt agatgacatt atggtctata agtataatga ttctaaattc
    13921 ttgcttgtag ttaatgcttc taataaagaa aaagattatg agcatataat gaaactattt
    13981 aaaaaaggta aatataattg tgatgtagtt gatatgtctg atgatatttc tttactagca
    14041 attcaaggac ctaatgcagc tagttttctt caaaaggatt tagtggataa gctagatgaa
    14101 cttaaaatgt atgactttaa gtattatagt attttaggaa tgaaattttt aatttctcgt
    14161 agtggttata ctggtggtga tggttttgaa atttatggta agaatgaaga tatcttaaaa
    14221 ctatatcaac acctaacaaa aagaggaatg aagtcatgtg gtttaggtgc aagagacact
    14281 ttaagatttg aagcagccct acctttatat ggccaagagt tagatatgga tattaatcct
    14341 ttagaagctg gtttaagttt cggtgttgat tttaataaag atttttatgg caaagaagct
    14401 ttaagcaaaa tcaaagaaga aggattaaaa agaagaagtg taggtttaga gttattagga
    14461 ccaggaattg caagacacgg ttataaagta tattctgaag gtaaagaaat tggttatgta
    14521 actactggtt atatgatacc taatactaaa gatagttacg cggttgcttt aatcgatcgc
    14581 gatgagtata aattagataa agaagtagaa atcttagttc gcaagaaatt tgtaaaggct
    14641 aagattatta agaaaaaatt catggataaa aaatatcaaa aataggagga gaaaatggca
    14701 gatattaaaa aaggtttgtt ttatacaaaa acacatgagt gggtaaaagt agaaggaaat
    14761 gttgcataca ttggcttaac agcttttgca gctagtgaat taggcgatat tatttatttt
    14821 gaaggaccag atgctgatga tgttgctcaa ggcgcagaat gtggcgttgt tgagtcagta
    14881 aaatcagctt cagatatcta ctcacctgta agtggaaaag tactagaagt aaacgaagcg
    14941 gttttggata gtccagaatt aattaatgaa gatccatatg gtgcttggtt agttaaagtc
    15001 gagcttgatt cgaaagaaga attaaagaaa ttactttcag ctgaagcata cgaaaaggaa
    15061 ttagctaatg cataagtatt tacctcatac agctagcgat attaaagaaa tgcttgataa
    15121 aataggtatc aagacaattg atgaattatt cgtagcttta ggagatatta aactgactaa
    15181 accattagac atccctagca agatgagtga aaatgagtta agagatcatt ttggaagact
    15241 agcaagtaaa aataaaaact tgctttcttt ttgtggttta ggtagttatg aaagctatga
    15301 cttcagtatt attgatgctc ttatttctcg ccaagagttt cttacatctt atacaccata
    15361 tcaaccagaa gtatcacaag gaacattgca atatatcttt gagttccaat cttatgtttg
    15421 tgagttaact ggtatggata tagctaatgc ctcaatgtat gatggtgcta gtagtacagc
    15481 tgaagctatc tttatggcag tagcagctac aaggaaaaat aaggttttac taagtaaaac
    15541 aattaatcct tatgtgttag aggttgttaa gacttactct aagtatcgtg atttagaagt
    15601 tgaatatgtt ttagaaaaag atttcagttt tgatttggaa gatttaaaga aaaagttaaa
    15661 taatgatgtt gcttgttttg tggcgcaaac accaaatgtt tatggtgttt tagaagacta
    15721 tagcaaagtg aaagcaactt tagaagaaat tggcgcttta tttattatta atcaagaacc
    15781 aagcagttta gctttattta aagctccaag tgaatataac gccgatatag cttgcgggga
    15841 tatgcaaagt ttaggtttaa agagaaactt tggtggacca agtgtaggct atattgctac
    15901 tatctctaaa tatcaaagaa gactacctgg caggattgtt ggtaagactg tagatgtaga
    15961 aggaaaaaga ggttatgttt taacacttca agcacgtgaa caacatatta gaagagaaaa
    16021 agctacttcg aatatttgtt ctaatcagtc tttaatggct ttaactgttt caatctattt
    16081 aagtttagtt ggtaaacttg gtttaattga actaagtaag agagcttata ataatgctca
    16141 ttatttagaa gaagagttat taaaaactaa gaaatttaaa aaagttactt ctaggccatt
    16201 ctatcaagaa tttgttttag aatatttagg caatcctaaa catttagagc aaaaactttt
    16261 agctttaggt tatttatctg gttataacat caaagataat ttaattattt tctatgcaag
    16321 tgaggtaaga actaaagacg aaatagatgg gtttgtaaag aaagtaggtg aagtataatg
    16381 tacgataaat taatttttga aagatcaaaa aaaggtcgtg taggtattac tttaccaaaa
    16441 actgatatta aagattacgc attaccaaaa gaacatttaa caaaaagaga tttattactt
    16501 cctgaagttt ctgaagttga tgttattaga cattatacta atttatcact taaaaactat
    16561 ggtgttgatc gaggtattta tcctttagga agttgtacga tgaagtataa tcctaagatt
    16621 aacgaagagt tatcaaagaa tcctaaattt aataatttac atccactaca accagatgtt
    16681 tcaagtcaag gagctttaga attagaatat aatttagaaa aattactaaa tgaaatttta
    16741 ggattaaaag cttttagttt tgcgccttgt gctggagcgc atggagagtt gactgggtta
    16801 atgatgatta aagcttatca tgagaaaaat aaagatttta aacgtaaaaa aattattatt
    16861 cctgattctg ctcacggaac taatccagca tcagttgcaa ttgtaggttt tga
//
LOCUS       DAOSGS010000007        16355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_2900_length_16355_cov_7.614479, whole genome shotgun sequence.
ACCESSION   DAOSGS010000007 DAOSGS010000000
VERSION     DAOSGS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2900_length_16355_cov_7.614479"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLQ22_00740"
     CDS             <1..184
                     /locus_tag="PLQ22_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58520.1"
                     /translation="HKGKTIYDIKVVSRKNQNEMISFKGTDFQMLLLEAAIYKKTGER
                     WRKSKKMWITKLEKKE"
     gene            300..677
                     /locus_tag="PLQ22_00745"
     CDS             300..677
                     /locus_tag="PLQ22_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOH58521.1"
                     /translation="MRQWMCDPRFMCNKHLFGEHVEHHMFIGTLERKKSINGYIKNDL
                     LEPLSLKARHDALADEIIKRNFNHYTPLVFEEETIFDYLREDQINHKIDKDNSLLLLL
                     SRCEVCQQRYDYFYYQALEKGEL"
     gene            674..892
                     /locus_tag="PLQ22_00750"
     CDS             674..892
                     /locus_tag="PLQ22_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58522.1"
                     /translation="MKDNLKTILLVAGFGIIVWLVIVFVFSAGIIILVLTVIGLAICG
                     AYRIWEGYAKEELLKKEENFDTLKEENK"
     gene            892..1344
                     /locus_tag="PLQ22_00755"
     CDS             892..1344
                     /locus_tag="PLQ22_00755"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOH58523.1"
                     /translation="MYGVTKSFKFEASHRLFTMPAEHPCANLHGHSYVVRVSVWVEDI
                     KNLENPNMVIDFGKLKEFQKQLDEHFDHSLILHEDDPIYDAVKDHVCRIIKMPNKLDV
                     TAENMAYLFANSINAICNKYKIKSGNIQVDVDETVGNTASYVREIALI"
     gene            1467..2153
                     /locus_tag="PLQ22_00760"
     CDS             1467..2153
                     /locus_tag="PLQ22_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58524.1"
                     /translation="MKTLQSELYRTGLINTYVEPKMESNFETLLINEALKSEVSDRCN
                     MIRKGIKEKFKLYNSKEKNGEVKILYSEKEGRKMKEEKYNFLKKFVSHLINNTQLKLK
                     DVLLLMRNESGGNENTIKNFLPVAKYCMPEFIKTNDGIWTITPTISADEFLAIYNQNK
                     NSYWKRYLKQKSDTSVVKRIERDSNNIEKKSFQMPLNDLVDFLKNNKIGIDVSGKVDV
                     NITVNIKFNK"
     gene            2213..2437
                     /locus_tag="PLQ22_00765"
     CDS             2213..2437
                     /locus_tag="PLQ22_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58525.1"
                     /translation="MAARNKWIWGASGTSKYAVKDLTEISIGFNPVLNSEKPFQVIAK
                     KRVGYQSVLLKNYSTQLDAQKFINETFQSR"
     gene            2622..2915
                     /locus_tag="PLQ22_00770"
     CDS             2622..2915
                     /locus_tag="PLQ22_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58526.1"
                     /translation="MKLELINEEEWIEEQVNILKDEFESYPNINIKTREDIEIWLDDT
                     DLIQILTENFDYDTILSEHIDFSNEEFSKQFNELFQVVKSKIINKFIEDVINV"
     assembly_gap    2972..3071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3542..4474
                     /locus_tag="PLQ22_00775"
     CDS             3542..4474
                     /locus_tag="PLQ22_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58527.1"
                     /translation="MFDITKIHPIINHLKAISNYYKETNNEIITFCNYCDDSTRHKAD
                     HGHLYISKSSPVFHCFRCSASGTLIRFLIDSGFEDQEVLTYLSQFIKYRTVKDYYKIK
                     KTVPRINQIKERVIKLNLEFEQNDFTKYKIFTNYLKSRLGNVNMSDFLISPMFFYEKL
                     SCLFTNSENEDVVLRLIEPYKDIRYHLFQETSGRYYFQDVDFDKYHRVVLTEGPFDML
                     SLYLYSDEFKDCFFISLNGKKYSSALEWLILEYFLIDHLEVNFVFDNDVTKYRTYLFR
                     AKALAKNYNENVIIRGWKPMIGKDTGDFPGIVEL"
     gene            4495..5466
                     /locus_tag="PLQ22_00780"
     CDS             4495..5466
                     /locus_tag="PLQ22_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58528.1"
                     /translation="MLQIVSQTNLDTVIFMSDDTPMYQVVGLNLGLLKKKFLCIGTEG
                     LNRSSKLIDGKYYDGLQARFTKSNSTSYNRYSANRTFFKRVVKDDEKHETMHLTIANM
                     YRHLDNISIQMKLNTPMKADQGVISQVEKLSSHVKISEKMQNLCDINLSSNIPLINKN
                     IKTSEEEEELKNTMDLAVYKTKVFEENYRAMYPLLVLKNFSGEGGGEHLTTFSEFERF
                     NKYSNMIKDVEKPSEDARRKFIELCEIFRLNKVIKNIKVNNFEVIQQDDYLKIRLFGI
                     TIAITTNLKHFLYFDHPGINFVADFIDKNDMLNNIEAKISKEIFTRI"
     gene            5475..6311
                     /locus_tag="PLQ22_00785"
     CDS             5475..6311
                     /locus_tag="PLQ22_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58529.1"
                     /translation="MIKDENGAIVVNWNDMYDPELVYSFLRDMRYTSVNDTHINSENR
                     FTTIEGDYNSTSSAGTGNFVIIPNEAKYEDKTVRFELIKRIADLAEFPEVDNFLLSHC
                     SYIKDWMDKNPEDKSIELAFKATVSRSDDLGTVTDYLTNVKLTKSIFEDSKRKLELSV
                     LVLAAIIKKFYKSDDKEFEIYNLTTDLQKFNSNNDVILNIMKNPIETLENYDIETSDV
                     NNKVLSYHNGKFSVNFKKFRFLQNIILITPIFEMYRQNLDLKFLSYLITMKKLEPQRS
                     LF"
     gene            6324..6521
                     /locus_tag="PLQ22_00790"
     CDS             6324..6521
                     /locus_tag="PLQ22_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH58530.1"
                     /translation="MKKIIPVFYSEYGRYINRFRAMPSFIDGLKPVERRVLLSLYKEG
                     RKKAIKSATIVGHCLGHPSSW"
     gene            6484..7695
                     /locus_tag="PLQ22_00795"
     CDS             6484..7695
                     /locus_tag="PLQ22_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH58531.1"
                     /translation="MWDIVWAIHPHGDQSLYGTLVNIVNQGYAKGEGNWGSPGLEDAP
                     PAAHRYTEVSLKKWVDELAFTYLDEKYVPYQEFELEPEPIYLPSPIPIGLIGHGVITG
                     ISFYRTLIPKYKLSDLSKRLLWILHGKKKKEEIIIQPNSHDCTVSEAEPGEFQKILTT
                     GIGSINYTPHGKLESKNIRIQGRAPNSSFNILVNDQEKLDINLIDNSSSQIDILVEPK
                     KKSTNLQNLATTVWQEYLIKKLNFNCLFCDNEGNVEAYGIDNILAYNYNLWKYSVQLK
                     NIDDFNKLSNRKVELMIVQIIRYIFEQYKSNTVEEIITHFKELKKNTDISIEIDIFDV
                     DNDIWKKEIKQILEQDIIDICNKRTIKNLVETVIDIQKVEKELLSAKSLIDNCEKNCL
                     DFITELSKGTA"
     gene            7692..7973
                     /locus_tag="PLQ22_00800"
     CDS             7692..7973
                     /locus_tag="PLQ22_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58532.1"
                     /translation="MNIKIIEEATKLIKAEVLYQVELDVDDKHQLTFDMLEIVDITNP
                     EEPIQNSIVEITNVVSETTDIDELKNIVSECKDYVEKHESEIIEMIRNR"
     gene            8044..8364
                     /locus_tag="PLQ22_00805"
     CDS             8044..8364
                     /locus_tag="PLQ22_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58533.1"
                     /translation="MIIEKLLNEILRILVTLLILLMILYALVPSANAETTYYILGDRN
                     VVRTYTPDPVGEDKIVYVDCKKLRSQDDVLGCIIAANYAKEKQLEKVASNKKRTPERV
                     FAKH"
     gene            8469..9134
                     /locus_tag="PLQ22_00810"
     CDS             8469..9134
                     /locus_tag="PLQ22_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOH58534.1"
                     /translation="MFCSVDNCDSKHYANGYCQRHYQQIKIHGKIITLVKRTPYDPNE
                     FIIDKNICWIILYNARGEEIAKAKIDVKYYDQIKEYKWRLHKKKYAVTTLYDLNNQPY
                     IGFLHQAIIQLSGQKVPDGFEIDHKDTDRLNCLEENLRICTHTQNLKNQGIRCDSTSG
                     YKGVSWNKQRKKFEAYIMENYKKIHLGFFDIADDAANVYNSAAIKYFGEFAKLNIIKE
                     NIK"
     gene            9131..10135
                     /locus_tag="PLQ22_00815"
     CDS             9131..10135
                     /locus_tag="PLQ22_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH58535.1"
                     /translation="MISPNISWFEKYRPQDINDMVFSNDAQKNIVKAWIDEETIPGNV
                     LLSGPAGTGKSTLALIAIRRIVKNQADLCRIKTRSVSDVDEKIAPFVQKKPVSSKCKI
                     VYIEEIDRTSRQFQAQLKEDLMEKYQDSVAFICCTNHPKRIDNALLSRFTFQIEFKSD
                     NIQDIKNRLEFILTQEKAQYDPDELVKFVTKNYKAGLRNLINSLHVSYLSHNGTIDFS
                     ALEQNLNIEDNIVGYIVGMIEKIMISTDPNAKKICIATPFNSIIAKEYSSFVTLCHNN
                     YDINYENVFERLYELIRYLPLQVIIGKYSEEIEMKKYPNVHLISCFYEMMKCALEVHL
                     "
     gene            10151..10330
                     /locus_tag="PLQ22_00820"
     CDS             10151..10330
                     /locus_tag="PLQ22_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58536.1"
                     /translation="MAVGISLEDFIEASIQAYRKGFNDAIRILIESEKTIDEKVMKES
                     IKEILTKQGKVKNEW"
     gene            10446..12254
                     /locus_tag="PLQ22_00825"
     CDS             10446..12254
                     /locus_tag="PLQ22_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH58537.1"
                     /translation="MALQALGGGIEPVRARPGMFIGDTQNPTHLAEEVLDNAIDEIAN
                     GFGTSIGIFNNLDDGSFWVSDNGRGLPMEPITLADGSVEDSVKVLCTELFSGTKFDTE
                     DYAQLIGMHGVGLVAVNALSDWLTIKTRDRKNKTRVVTYSFIDGELQQISEETDNDFS
                     YSTVVGFKPSKKYFGSLEFDNRYFVERLISVQSIFDLNGFTFNSKPIPKLSFESYIKQ
                     HLALAEEDQLFSLSFSTKDNSNHIKLYATYVDQDDSITLGNVNLRTCDGKFINSFHTE
                     LKKSINEKIDKSFSKINDREYLNGLRAFIMINVPEPKFDSQTKVRMVLDVKKILIDPL
                     KGQLDWFVEQIIDTLQNNLERKLHQKILNGSSRSARANKRVSISKLHDCQHIPGDVLY
                     IVEGDSAEGTLKQITNKKIEACYPLKGKVLNVEAATLDKIQKNKEIGDLLKALGSVSN
                     RRYKSIKILSDADVDGYHIAVLMLLAIAKFAPDYIKSGNLSVIIPPLYGAEKGGKYYP
                     IYDQIKIDQFKKDNYKIRRFKGLGEMNPNQLEACIRSGFEYKIKWPDSDKQLNNLISI
                     ITNTDLKRAIMNAEGVKIDIILAEINKQFSQKTKTI"
     gene            12268..12702
                     /locus_tag="PLQ22_00830"
     CDS             12268..12702
                     /locus_tag="PLQ22_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58538.1"
                     /translation="MANFPFPPKAKAAPEAAAPAPAAPAAEKAPKQKKSGADRKKPAP
                     AMTSEQVKQIIALVKDHSYSQIAETVGVTKFQVNRVLMATKVQLRENAKGDAAKLAKV
                     EEYIKTYLNRPEDSRPGKGGGRGGKVKGALDSVVGDILASIG"
     gene            12779..13192
                     /locus_tag="PLQ22_00835"
     CDS             12779..13192
                     /locus_tag="PLQ22_00835"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOH58539.1"
                     /translation="MKHTEANLYTDAGSRGNPGPSGVGVVLTTTDNKSIAEMNIFLGT
                     TTNNAAEYMGLIHGLQIAQQHGISRIHIFMDSMLIVNHIKGIYKVRNSRLEPLYKRVQ
                     ELLKLFGDGYTIEHIERAKNARADKLANEAMDRGY"
     gene            13195..13437
                     /locus_tag="PLQ22_00840"
     CDS             13195..13437
                     /locus_tag="PLQ22_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58540.1"
                     /translation="MDRTELNSNEPHSAVNSAINYISQKINDPVLLESLASCAIESNR
                     LAEVCLGTINRIKNKQPVSDRYLLGLAWFLKSIEKG"
     gene            13441..13635
                     /locus_tag="PLQ22_00845"
     CDS             13441..13635
                     /locus_tag="PLQ22_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58541.1"
                     /translation="MQKLLKVKINIDESNSKICNVNCHYLDTICDECKLFNKALCGKI
                     DPEIWFRCDECLNQEIKDEA"
     gene            13625..14125
                     /locus_tag="PLQ22_00850"
     CDS             13625..14125
                     /locus_tag="PLQ22_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58542.1"
                     /translation="MKLKLKVFDPTIKEMTMGDKIHKNILIKNNNNDRWNKEIEININ
                     EKLILYLIEHIIDSMVENKMFVIDAINRFRVPSIMLNDIDKIIKSARAWKSIQIKLVN
                     MKYETGKFTRISLFEELINQKKIKTSVQDAIEAKINSKIVPHGVPVLEKIEEIEPIQE
                     DYNFNI"
     gene            14339..15151
                     /locus_tag="PLQ22_00855"
     CDS             14339..15151
                     /locus_tag="PLQ22_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58543.1"
                     /translation="MEGVLERRFNEIGARVKFTNLSRRDLQSDLKIVVDVKRDKNGEF
                     FEVASDSFENIVIPNVVPKDRHLLLIHKDDSKRIINKVKFLCGHDETHWFAASIPESV
                     RNVVDVDSAKQALKPKEVVLVESSKGLTKSKPKAHLHRNSARVRQGEWFFIPIERNFN
                     QMEILKNEPIRRGRGKPHVCEELVRFGGNTVMVNSRFPNGITVGEYDNLIKSDPKMAK
                     FPWRRMTRDMKVFVRGKIRHPDHKTISLRIWHEVIPNEETKARSNRNLAFLD"
     gene            15250..15573
                     /locus_tag="PLQ22_00860"
     CDS             15250..15573
                     /locus_tag="PLQ22_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58544.1"
                     /translation="MFLSNQKEGIYAFVDDAVADVKPNWWRVTFQILLPTPDFKPLKK
                     MWILDNQQIHGQEFTMGGVKYQLQKTDLKLANQPSQHQTTSKIPEFEIKKRKRDIPSY
                     LKLVK"
     gene            15676..>16355
                     /locus_tag="PLQ22_00865"
     CDS             15676..>16355
                     /locus_tag="PLQ22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH58545.1"
                     /translation="MQNLSIEEFNNLDISPYQIYNLDLDYQQSFSINSYLENKNDTID
                     NDQLLNILFADIEVFTNNAGVFPKPEIAKFPINAITIYSTFEKIFRSYFLLQHSNINK
                     FPSKNEIPDLINQFTKDLLVDKYINSDEKLEIYMFTSEMDLIRACWGKIRQIDPSVLS
                     GWNSDHFDLPYIYFRLSNLLNKNEVEIGKILSLFGKIKVEKFGNEFLIKIPEMPVSDL
                     MYLYKPRD"
BASE COUNT         6277 a         2102 c         2850 g         5026 t
ORIGIN      
        1 acataaagga aaaacgattt atgatattaa agtcgtctca agaaagaatc aaaatgaaat
       61 gatatccttt aaagggacag acttccaaat gctattattg gaagctgcaa tatataagaa
      121 aactggtgag agatggcgaa agagtaaaaa aatgtggatt actaaattag aaaaaaaaga
      181 atagtgaaat tttatatcgg caagatctga aactagatct tgccgaattt tttattattt
      241 aaaactttta tatagaaata attctatcaa aatgaatatt tttatttgga gagaactgaa
      301 tgcgacagtg gatgtgtgac cctagattca tgtgtaacaa acatttattt ggagaacatg
      361 tagaacatca catgttcatt ggtacattag agagaaagaa atcaattaat ggttatatta
      421 aaaatgattt attagaacct ttatcattaa aagctcgaca tgatgctctt gccgatgaaa
      481 ttatcaaaag aaattttaat cattatactc ctttagtatt tgaagaagaa actatctttg
      541 attatttacg agaagatcaa attaatcata aaatagataa agataattct ttattattgt
      601 tattgtccag atgtgaagta tgtcaacaaa gatacgatta cttttactat caagcattag
      661 aaaaaggaga actatgaaag ataatttaaa aactatttta ttggtagctg gttttggaat
      721 aattgtgtgg ctagttattg tatttgtctt ttcagctgga ataattattc ttgtactaac
      781 cgtaatagga ttagcaatat gtggagcata tagaatctgg gaaggttatg caaaagaaga
      841 attgttgaaa aaggaagaga attttgatac tttgaaagag gagaataaat aatgtatggt
      901 gtaacgaaat catttaaatt tgaagcatca catagattat ttacaatgcc agcagagcat
      961 ccttgtgcta atttgcatgg tcattcatat gttgtaagag tgagcgtttg ggtagaagat
     1021 attaaaaatc tcgaaaaccc aaatatggtt atcgattttg gtaaattaaa agaatttcaa
     1081 aaacagttag atgaacattt tgatcattct ctaatattac acgaagatga tccaatatat
     1141 gatgctgtta aagatcatgt atgtagaatt atcaaaatgc caaataagct agatgtaaca
     1201 gctgaaaata tggcttactt atttgccaat tcaattaatg ctatttgtaa taagtataaa
     1261 attaagagtg gtaatattca agttgatgtt gatgaaacgg taggaaatac tgctagttat
     1321 gttagagaga tagctttgat ataaatattt aatgtcggca agagctagaa taaagctctt
     1381 gccgacaatt ttttattcgt tctttttata tagaaataat atgaacatat taatattgat
     1441 tattatttca aaagaaagga gatggaatga aaacacttca atcagaactc tacagaactg
     1501 gattaataaa tacatatgta gaaccgaaaa tggaatcgaa ttttgaaaca ttattaatta
     1561 atgaagcttt aaaatctgaa gtgtcagatc gatgtaacat gatccgcaaa gggatcaaag
     1621 agaagttcaa gttgtataac tcgaaagaaa aaaatggaga agtcaagata ttgtactccg
     1681 agaaagaagg tagaaaaatg aaggaagaga aatataattt tctcaaaaaa tttgtcagtc
     1741 acctaattaa taatactcaa ttgaaattaa aagatgtttt attattgatg agaaatgaat
     1801 ctggcgggaa cgaaaacacc atcaaaaact ttttaccagt tgcaaaatat tgcatgccag
     1861 agtttataaa gactaatgat gggatttgga caattactcc aacaatttca gcagatgaat
     1921 ttttagcaat atataatcag aataaaaata gttactggaa gagatatttg aagcagaaat
     1981 cagacacttc agtggtaaaa cgaatagaaa gagactcaaa taatattgag aaaaaatctt
     2041 ttcaaatgcc attaaacgat cttgttgatt ttttgaaaaa caataagatt ggtatagatg
     2101 tgtccggaaa agttgatgtt aatataacag ttaatatcaa atttaataaa taatcaattt
     2161 agcaagatct gaaactggat cttgctaaat tttatttgaa aggagataaa taatggcggc
     2221 tagaaacaag tggatctggg gagcttctgg aacatcaaaa tacgcagtaa aagatctaac
     2281 agaaattagt attggattta atccggtatt gaattctgaa aaaccatttc aagtaatagc
     2341 taaaaaacgg gttgggtatc aaagtgttct attaaaaaat tattcaacac aattggatgc
     2401 tcaaaaattt ataaatgaaa catttcaatc gagataaaaa ataaggggga atttttactc
     2461 atgaaaatta ttatagagtg gattatagac aatatacttt caaaaatatt taagtcgaaa
     2521 aatgatctaa aaatagcaga attgacacca atacaacgtt caatttggtt atcaattaat
     2581 aatcataaac gagatagaac aagccatttg agataaaaaa gatgaagcta gaacttatta
     2641 atgaggaaga atggatagaa gaacaagtaa atattttaaa agatgaattc gaaagttatc
     2701 caaatattaa tattaaaaca agagaagata ttgagatttg gttagatgat actgacctaa
     2761 ttcaaatact aactgagaat tttgattatg atactatcct tagtgaacat attgattttt
     2821 ctaatgaaga attctcaaaa caattcaatg aattatttca ggtcgtaaaa agtaaaataa
     2881 tcaataaatt tattgaggat gttataaatg tatagtatta tcggcaagag ctagaataaa
     2941 gctcttgccg atttttaatt ttctcaattt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn naatttcgac taggtcgaag agctgtaatc actggagcgc cttggtcctc
     3121 agcgtcatga ggaccacctt cttttggaga agactatatt aaactaacaa taatttaata
     3181 tatcttgaat atattaaatt aatggtctga aacgatcaaa agttagaatt taatagtcaa
     3241 ctcttaaaga tcaaaaatga tacaaataaa gattaagaaa agcctcggag cataataaaa
     3301 atattatgca atgaaggggc taatcaaatc ttaacaattt ttaaaatccc agaataaagg
     3361 gaaatttggc atcctccagt atggtcagca gtcacctctg ggctgatctg gtcgaattat
     3421 ttatattaca attgtcgcta tggaaaaaat ccatagcgac aattttgatt ttttctattt
     3481 ttatatagaa ataatactat catagtgata taatcatatt tttgtttagg agttttatgg
     3541 tatgtttgac atcacaaaaa ttcatccaat tattaaccat ttaaaagcaa tttctaatta
     3601 ctataaagag actaataatg agataatcac gttctgtaat tattgtgatg attcgacacg
     3661 tcataaggct gatcatggac atttatatat atcaaaatca tctccagtat ttcattgttt
     3721 cagatgtagt gcatctggaa ctttaattag atttttaata gattctggat ttgaagatca
     3781 ggaagtattg acatatttat ctcaatttat taagtataga actgtaaaag attattataa
     3841 gataaaaaag acagtaccaa gaattaatca aataaaagaa agagtgatca agttaaattt
     3901 ggaatttgag caaaatgatt tcacaaagta taagatattt acgaactatt taaaatcaag
     3961 attaggaaat gtaaatatgt cagatttcct aattagtcca atgttctttt atgagaaatt
     4021 gagctgttta tttacaaaca gcgaaaatga agatgttgtt ttaagattaa tagagccata
     4081 taaagacatt agatatcatt tatttcaaga gacttctgga agatattatt ttcaagatgt
     4141 agattttgat aaatatcaca gagttgtgtt aactgaagga ccattcgaca tgttatcatt
     4201 atacttatat agtgatgaat ttaaagattg tttctttata tctttaaacg gcaagaaata
     4261 tagtagtgca ctcgaatggt taattttaga atatttctta attgatcatt tggaagttaa
     4321 ttttgttttc gataatgatg ttacaaaata tagaacatat ttgtttagag ctaaagctct
     4381 tgcaaagaat tataatgaaa acgttataat aagaggttgg aagccgatga taggaaaaga
     4441 tactggtgat tttccaggaa tagtagagtt ataaaaaata tgaaaggata aagaatgtta
     4501 cagattgtat ctcaaacaaa cttggatact gttattttta tgtcagatga tactccaatg
     4561 tatcaagtag taggtttaaa tctggggtta ttaaaaaaga agtttttgtg tattggaaca
     4621 gagggattaa atcgctcttc taaattaatt gatggtaaat attatgatgg actacaggca
     4681 agatttacaa aatctaattc aacatcttat aatcgttact ctgcaaatag aacatttttt
     4741 aaaagagtcg ttaaagatga tgaaaaacat gagacaatgc atcttactat agcaaatatg
     4801 tatagacatt tggacaatat tagtattcag atgaaactta atacaccaat gaaagcagac
     4861 caaggcgtaa tttcgcaagt cgaaaaatta tctagtcatg taaaaatatc tgagaagatg
     4921 cagaatttgt gtgatattaa tctttcctca aatataccac ttataaataa gaatataaag
     4981 acatcggaag aagaagagga attaaagaat acaatggatt tagcagtcta taaaaccaaa
     5041 gtctttgagg aaaattatag agcaatgtat ccgttgttag tgttaaaaaa cttttctgga
     5101 gaagggggag gagaacatct gacgacgttc tctgagtttg agagatttaa taaatattct
     5161 aatatgatca aagatgttga aaaaccatca gaagatgctc gtagaaaatt tattgaactg
     5221 tgtgaaatct tcagattaaa taaagttatc aaaaatataa aagttaataa tttcgaggtc
     5281 attcaacaag atgactattt gaaaattcga ctttttggta taacaatagc tataacaact
     5341 aacttaaaac attttctata ttttgatcac ccaggaatta attttgttgc agattttatc
     5401 gacaaaaacg atatgctgaa taatattgaa gcaaaaatca gcaaagaaat ctttacaaga
     5461 atctagagga caacatgata aaagatgaaa atggagcaat agtagttaat tggaatgata
     5521 tgtatgatcc tgaattagta tattcttttt taagagatat gagatatact tccgttaatg
     5581 atacacatat caattcggaa aatcgtttta caactattga aggtgattat aattcaactt
     5641 catcagctgg aactgggaat ttcgtaataa ttcccaatga ggcaaaatat gaagataaaa
     5701 ctgtaagatt tgaattaatt aagcgaatag cagatcttgc tgaatttcca gaagtagata
     5761 attttctatt gtctcattgt agttacataa aagattggat ggacaaaaat cctgaagata
     5821 aatcaataga attagcattc aaagcaacag tttcaaggag tgacgatctt ggaactgtca
     5881 ctgattattt aacaaacgta aaactaacaa aatcaatatt cgaagattca aaaagaaaat
     5941 tagaattatc tgttttagtt cttgccgcta ttattaagaa attttataaa tcagatgata
     6001 aagaatttga aatttacaat ctcacaactg atttacaaaa attcaatagc aacaatgatg
     6061 taatactaaa tattatgaaa aatccaatag aaacattaga aaattatgat attgagacat
     6121 cagatgtgaa taataaagtt ctctcgtatc ataatggtaa attttctgtt aattttaaaa
     6181 aatttagatt tttgcaaaat ataattctta ttacaccaat ttttgaaatg tatcggcaga
     6241 atctggattt gaaatttctt agttatctta taactatgaa aaaacttgaa cctcaaagat
     6301 ctctatttta aagaggagta acagtgaaga aaataatacc agttttttat agcgaatatg
     6361 gaagatatat aaatagattt agagcaatgc cttcatttat tgatggatta aaacctgttg
     6421 aaagaagagt tttattatcc ctttataaag aaggtagaaa gaaggcaatt aaaagcgcca
     6481 caattgtggg acattgtttg ggccatccat cctcatggtg atcaaagtct ctatggtact
     6541 cttgttaata tcgtaaatca aggatatgct aaaggagaag gaaactgggg atcaccaggg
     6601 ttagaagatg cacctccggc agctcacaga tacactgaag tttctctcaa gaaatgggtt
     6661 gatgaactag catttacata tcttgatgaa aaatatgttc cataccaaga gttcgaatta
     6721 gaacccgaac caatttatct accaagtcca attccaattg gtttaattgg acacggagta
     6781 attaccggta tatcattcta tagaacctta attccaaaat ataaactttc agatctttca
     6841 aaaagattat tatggattct tcatggaaag aagaaaaaag aggaaataat aattcaacca
     6901 aattcgcatg attgcacggt tagtgaagct gaacctggag aatttcaaaa aattctaaca
     6961 accggaattg gaagtattaa ctatactccg catggaaaat tagaatctaa aaatatcaga
     7021 attcaaggaa gagctccaaa ttcttcattc aatatattag taaatgatca agaaaaatta
     7081 gatatcaatt taatagataa ttcttcaagt caaatagata tattagtcga acctaagaaa
     7141 aagtcaacaa atttacaaaa tttagctaca acagtttggc aagaatatct aattaagaaa
     7201 ttaaatttta attgtttatt ttgtgataat gaaggaaatg tagaagctta tggaattgat
     7261 aatatcctag cttataatta taatctttgg aagtattctg ttcaactcaa gaatatagat
     7321 gatttcaata aattgtccaa taggaaagtt gagttaatga ttgttcaaat tattagatac
     7381 atttttgagc aatataaatc caatacagtt gaagaaatta ttactcactt taaagagtta
     7441 aaaaagaata cagatataag tattgagatt gatatttttg atgttgataa tgatatatgg
     7501 aaaaaagaaa ttaaacaaat tcttgaacaa gatattattg atatatgtaa taaaaggaca
     7561 attaagaatt tggttgagac agttattgat attcagaaag ttgaaaaaga attattatcg
     7621 gcaaaatctc ttattgataa ctgtgaaaag aattgtcttg attttataac agaattatcc
     7681 aaaggaacag catgaatata aaaatcattg aagaagcaac aaagttaata aaagcggaag
     7741 tattatatca agttgaatta gatgttgatg ataaacatca attaacattc gacatgcttg
     7801 agatagttga tataactaat cctgaagaac ctattcagaa ttctattgtt gaaataacaa
     7861 atgtagttag tgaaactaca gatattgatg aattaaagaa cattgtttct gaatgtaaag
     7921 attatgttga aaaacatgaa tctgagatta ttgaaatgat tcgaaacagg taaaaatttg
     7981 atattctaac ctttcatata gaaattatat agtatataat taaaaattga aaggagaatt
     8041 atcatgatta tcgaaaaact tttgaacgag attcttcgta tactggtaac tctgttgatt
     8101 ctgctgatga tcctgtacgc actggttcca tcagcaaacg cagaaacgac ttattacatt
     8161 cttggagaca ggaatgttgt aaggacgtat actcctgacc ccgtcgggga ggacaaaatc
     8221 gtctatgtcg actgcaagaa attgcggtca caggatgatg tcctcggctg cattattgca
     8281 gctaattatg caaaggagaa gcagcttgaa aaagtcgctt ctaataaaaa gcgaacgccc
     8341 gagagggtat tcgctaaaca ctaaacacaa agtggagtca gaaatgactc cactttcaat
     8401 ttttaatatt ttcaattttt atatagaaat aattaaattg tatttttaat ttggaaggag
     8461 atttataaat gttttgttct gttgataatt gcgattcaaa gcattatgca aatggatatt
     8521 gccaacgtca ttaccaacaa attaagattc acggaaaaat cattacgctg gtaaaaagaa
     8581 caccatatga tcctaacgaa tttattatag ataaaaatat ttgttggatt attttataca
     8641 atgctcgagg tgaagaaatt gcaaaggcaa agattgatgt taaatattat gatcaaataa
     8701 aagaatataa atggcgttta cacaaaaaga aatatgccgt cacgacacta tacgatctca
     8761 ataatcaacc atatattgga ttcttacacc aagcaattat acaattgtct ggacaaaaag
     8821 ttccagatgg atttgaaatt gatcataaag atacggatag attaaattgt ttggaagaga
     8881 atttgagaat ttgtactcat actcagaact taaaaaatca aggaattcga tgtgatagta
     8941 ccagtggata caagggtgta agttggaata aacaacgcaa aaaatttgaa gcatatataa
     9001 tggaaaatta taagaaaata catcttggat tttttgatat tgctgatgat gcggcaaatg
     9061 tatataatag tgctgcaata aaatattttg gcgaatttgc aaaattaaac ataattaagg
     9121 agaatataaa gtgatcagtc caaatatttc atggtttgaa aaatacagac ctcaagatat
     9181 caatgatatg gttttctcaa acgacgcaca gaagaatatt gtaaaagctt ggattgatga
     9241 agaaactata cctggaaatg tactactatc aggacccgct ggaaccggca aatcgacatt
     9301 agcactaatt gctattcgac gaattgttaa gaatcaggct gatttatgta gaattaaaac
     9361 tcgttcagtt tctgacgtcg atgagaagat cgcgccgttt gtacaaaaga aaccagttag
     9421 tagtaaatgc aagattgtct atattgaaga aattgatcgt acttctcggc aatttcaggc
     9481 tcaattaaaa gaagacttga tggaaaaata tcaagattca gtagctttta tttgttgtac
     9541 aaatcatcca aagcggattg acaatgcatt actatcaaga tttacatttc agatagaatt
     9601 taaatctgat aatattcaag atattaaaaa cagattagag tttattttaa ctcaggaaaa
     9661 ggcacaatat gatccagatg aattagttaa atttgttaca aagaattata aagctggatt
     9721 aagaaattta atcaatagct tacatgtgtc atacttgtct cataatggaa caattgattt
     9781 ttctgcatta gaacaaaact taaatattga agataatata gtaggttata ttgttgggat
     9841 gatagaaaaa attatgatct caacagatcc taatgcaaaa aagatttgca ttgcaactcc
     9901 atttaattca attattgcaa aagaatattc atctttcgta actctttgtc ataacaatta
     9961 tgatattaat tacgaaaatg tgtttgaaag actatatgaa cttattagat atttaccgtt
    10021 acaggttatt atcggcaagt attcagaaga gatcgagatg aaaaaatatc ctaatgttca
    10081 tttgatatca tgtttttatg aaatgatgaa atgtgcttta gaggtacatt tatagaaagg
    10141 aaaagatata atggcggtcg gaatatcatt agaagatttt atcgaagctt ctattcaagc
    10201 atacagaaaa ggattcaatg atgctattag aattttaata gaatctgaaa agacaataga
    10261 tgaaaaggtt atgaaagaat ctattaaaga aattctcacg aaacaaggta aagttaagaa
    10321 tgagtggtaa ggtgtcaaca attgacaaaa tactttcccc cttcattttt ctgatattca
    10381 aaaattttat atagaaataa tatttttaat aagttagttt attttttgtt taaggagaaa
    10441 aataaatggc tttacaagca cttggtggtg gaattgagcc tgtcagagca agaccaggca
    10501 tgtttatcgg tgacacacag aatccaaccc atctcgcaga agaagtacta gataacgcta
    10561 ttgacgaaat agcaaatgga tttggtacta gtattggtat attcaataat ctcgatgatg
    10621 gttcgttttg ggtaagtgat aatggaagag gtctgccaat ggaaccaatt actcttgcag
    10681 acggatcagt tgaagattca gtaaaagttt tatgtacaga attgttttct ggaacaaaat
    10741 ttgatactga agattatgca caactgattg gaatgcatgg tgttggtcta gtggcagtta
    10801 atgctctatc ggactggcta accattaaaa caagagacag aaagaataaa acaagagttg
    10861 taacttattc atttatagat ggtgagttac aacagatttc ggaagaaaca gataatgatt
    10921 tttcttactc tactgttgtc ggatttaaac caagtaaaaa atattttggt tcattagaat
    10981 tcgacaatag atattttgta gaaagattaa tatcagttca atcgattttt gatttaaatg
    11041 gatttacatt taatagtaaa cctattccga aattgagttt tgaatcttat attaaacaac
    11101 atttagctct tgccgaagag gatcaattat tctcattatc attttcaact aaagataatt
    11161 caaatcatat taaattatat gctacatatg ttgatcaaga tgattctatt acattaggta
    11221 atgtcaatct aagaacatgt gatggtaaat tcatcaattc ttttcatact gaattgaaaa
    11281 aatcaataaa tgaaaagata gataaaagtt tctcaaaaat aaatgataga gagtatttaa
    11341 atggtttgag agcttttatc atgataaatg ttccagaacc aaaatttgac agccaaacca
    11401 aagttagaat ggtattggat gttaaaaaga ttctaattga tcctttaaaa ggccaattag
    11461 attggtttgt tgaacaaatt attgatacgt tacaaaataa cttagagaga aaattacacc
    11521 aaaaaatatt aaatggttcg tcccgatcgg caagagctaa taaaagagtt tcaattagta
    11581 aattacatga ttgccaacat attccgggag atgttttgta tattgttgaa ggagattccg
    11641 ctgaaggaac tctaaaacaa attacaaata aaaagatcga agcttgttat ccattaaagg
    11701 gtaaagtatt aaatgttgaa gccgcaacgt tagataagat ccaaaaaaat aaagaaattg
    11761 gtgatttgtt gaaagcgtta ggatcagtaa gtaaccgaag atataaatca attaagattc
    11821 tatccgacgc tgacgtagat ggatatcata tagcagtatt aatgctgtta gctatcgcta
    11881 aatttgcacc agattatatt aaatctggaa atctatcagt aataatacct ccattgtatg
    11941 gagctgaaaa aggaggcaaa tactacccaa tatatgatca aataaaaatc gatcaattca
    12001 aaaaagataa ttataaaatt cgaagattca aaggattggg tgaaatgaat cccaatcaat
    12061 tagaagcttg tattagatca ggttttgaat ataaaatcaa atggccagat tctgataaac
    12121 agcttaataa tttaattagc attattacaa atacagattt aaagagggca attatgaacg
    12181 cggaaggtgt taagatagat atcatcttag cggaaatcaa taaacaattc tcacaaaaaa
    12241 caaaaactat ttaggaggca acaagttatg gcaaatttcc cattcccccc gaaggcaaag
    12301 gcagctcccg aagcagcagc tccagctcca gcagctccag cagctgaaaa ggccccgaag
    12361 cagaagaagt ctggtgcaga tcgaaagaaa cctgctccgg cgatgacttc cgaacaggta
    12421 aaacagatta ttgctctggt caaagatcac tcctattctc agattgctga gactgttggt
    12481 gtgacaaaat tccaggttaa tcgtgttctc atggctacga aggttcagct tcgtgaaaac
    12541 gctaaaggcg atgctgctaa actggcgaag gtcgaagagt atattaagac atatctcaat
    12601 cgtcctgaag attctcgccc tggtaagggt ggaggtcgtg gtggcaaggt taaaggtgct
    12661 ctggacagtg tggtcggaga cattcttgct agcatcggtt aattattaac taaccacata
    12721 actctcttga agaggaagaa attcctcttc aagagatttt ttatttgaag gaatgaatat
    12781 gaaacacaca gaagctaatc tatatacaga tgctggttct agaggaaatc caggaccatc
    12841 aggagttggc gttgtgctca ctacaacaga caacaaaagt atcgcagaaa tgaatatatt
    12901 tcttggaaca actacaaaca atgcagcgga atacatggga ttaattcatg gattacaaat
    12961 agctcaacaa catgggattt ctagaattca tatctttatg gattctatgc ttattgttaa
    13021 ccatattaaa gggatataca aagttcgtaa ttcaagacta gaaccactat ataaaagagt
    13081 tcaagagtta ttaaaactct ttggagatgg ttatactatc gagcatattg agagagccaa
    13141 gaatgctcgc gccgataaac ttgcaaatga agcaatggat agaggttatt aaaaatggat
    13201 agaactgaac taaattctaa cgaaccacac tcagcagtca attccgctat taattatatt
    13261 tctcaaaaga ttaatgatcc agtactgtta gaatctcttg catcatgtgc aattgaaagt
    13321 aacagacttg cagaagtttg tctcggaaca attaatagaa tcaaaaacaa acaaccagtt
    13381 agtgatagat atttattggg tcttgcttgg ttcttaaaaa gtatcgaaaa aggataaaat
    13441 atgcaaaaac tcttaaaagt taaaataaat atagacgaat caaattcaaa aatatgcaat
    13501 gttaattgtc attatttaga cactatttgt gatgaatgta aattatttaa caaagcatta
    13561 tgtggtaaaa ttgatccaga gatatggttt agatgtgatg aatgtttaaa tcaggagatt
    13621 aaagatgaag cttaaattaa aagtatttga tccaacgatc aaagaaatga caatgggtga
    13681 taaaattcat aagaatattc taataaaaaa taacaacaat gatagatgga ataaagaaat
    13741 tgaaattaat attaatgaaa aattaatatt atatcttatc gaacacatca tagatagtat
    13801 ggtggagaac aaaatgttcg ttatagatgc tattaataga tttcgtgtcc catcgataat
    13861 gttgaatgat attgataaaa ttattaaatc ggcaagagct tggaaatcga tacaaattaa
    13921 actagtaaat atgaaatatg aaactggaaa atttactaga attagcttat ttgaagaatt
    13981 gataaatcaa aagaaaatta aaacctctgt acaagatgct atcgaagcta aaattaacag
    14041 taagatagtt cctcatggag taccagtttt agaaaaaata gaagagatcg agccgataca
    14101 agaagattat aattttaata tttaaatttt ttatatagaa ataatactat tatattaatt
    14161 aaatgttttt actttatccg gcgatttaaa agaacgaggg cgagtggagg cattgagaga
    14221 taacttctct cttaaggtgt aaccctgtcc agaactccca taactcgtgc atgaggtatg
    14281 gatggtgtag gtgcccacca gccggataaa gtaaaatttt aaattgaaag ggggaaatat
    14341 ggaaggagtt cttgagcgtc gatttaatga gattggcgcg agagtgaagt ttacaaatct
    14401 ttctcggaga gatttgcaaa gtgaccttaa aattgttgtt gatgttaaga gagataagaa
    14461 cggcgaattt ttcgaagtag cttctgactc ttttgaaaac atcgttatcc ccaacgttgt
    14521 ccccaaggac cggcacttgt tgctgattca caaggacgac agtaaaagaa ttatcaataa
    14581 ggtgaaattt ctttgcggtc acgacgaaac gcattggttc gcagcttcga ttccggagtc
    14641 tgtgagaaat gttgttgacg ttgatagcgc aaaacaggcg ctgaagccca aagaagttgt
    14701 gttggttgaa tctagtaaag gtttgaccaa atcgaaaccc aaggcacatc tgcacagaaa
    14761 tagtgcaaga gtgcgtcagg gagaatggtt cttcattccg attgaacgta atttcaatca
    14821 gatggagatt ctgaaaaatg aacctattcg acgcggtcgc ggcaagcctc acgtttgtga
    14881 ggagcttgtc cgctttggag ggaacactgt aatggtgaat tcgagatttc ctaacggaat
    14941 tactgttggg gaatacgaca atctcatcaa aagtgatccc aaaatggcga aatttccttg
    15001 gcggaggatg actcgcgaca tgaaagtgtt cgtgagaggc aaaattcgcc atcccgatca
    15061 caaaaccata tctcttcgga tttggcatga agtcattccg aatgaggaaa caaaagccag
    15121 gtccaatcgc aatcttgcgt ttctggacta agttacaaaa tggtgagcag ataaatttct
    15181 gttcaccatt ttttattttt cttaaaggag atactatgga tgattctcta gttattattc
    15241 ctggaaacat tgttcttatc aaatcaaaaa gaaggaattt atgcatttgt agatgatgct
    15301 gttgcagatg ttaaacctaa ttggtggaga gtaacttttc aaatacttct accaacacca
    15361 gattttaaac cacttaaaaa gatgtggata ttagataatc aacaaatcca tggacaagag
    15421 tttacgatgg gaggagttaa gtatcagctg caaaaaactg atttaaaatt agccaaccaa
    15481 ccttcacaac atcaaacaac ctcaaaaatt ccggagtttg aaataaaaaa acgtaaaaga
    15541 gacataccat catatttgaa attagttaaa tagaaatcgt gggagagcag tctcccactt
    15601 aattttattt ttctctattt ttatatagaa ataataaatg taagttttta atttatgaaa
    15661 aaagaaagga gtagaatgca aaatctatct atagaagaat ttaataatct agatatttca
    15721 ccatatcaaa tctataattt agatctagat tatcaacaat cattttcaat taatagctat
    15781 ttagaaaata agaacgatac aatcgacaat gatcaattat taaatatttt gtttgcagac
    15841 attgaagtgt ttacaaataa tgctggagtg tttcctaaac cagagatagc aaaatttcca
    15901 ataaatgcta taacaattta tagtacattc gaaaaaatat tcagatcata ttttttatta
    15961 caacattcta atattaataa gtttccaagt aaaaatgaaa ttccagacct aataaatcaa
    16021 tttacaaaag atttactagt agataaatat attaattctg atgagaagtt agaaatttat
    16081 atgtttacta gtgaaatgga tttaattaga gcttgttggg gaaaaattag acagattgac
    16141 ccaagtgtgt taagtgggtg gaattctgat cattttgatc tcccatatat ttattttaga
    16201 ttaagtaatt tattaaataa gaatgaagtt gagatcggca agatcttatc tttattcgga
    16261 aagattaaag ttgaaaaatt cggtaatgag tttttaataa agatcccaga gatgccagtt
    16321 tcagatctta tgtatttata caaaccaaga gacga
//
LOCUS       DAOSGS010000008        14992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_3269_length_14992_cov_4.367343, whole genome shotgun sequence.
ACCESSION   DAOSGS010000008 DAOSGS010000000
VERSION     DAOSGS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3269_length_14992_cov_4.367343"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1222
                     /locus_tag="PLQ22_00870"
     CDS             65..1222
                     /locus_tag="PLQ22_00870"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOH58546.1"
                     /translation="MKNRVKIPKLLFFQEGPGVRNYQYTTEGVKLLNVANLVNGNLDL
                     STSERYISEEEAYGKYKHFLCDEGDLIIASSGIKVDYFDKKMGFVKPEHLPLCMNTST
                     IRFKSLDREVLDINYFMYFLKSQAFKKQLARQITGSAQLNFGPSHLSKMSFSLIDMED
                     QINIVKQLGKIELIIEMKTKQIQEYDQLIKSRFVEMFGDIFTNEKRYRIGVFNDFVTQ
                     MNIGPFGSALKNDYFVSKDDGYCMVYEQKHAIRKNIYVDRRYVDLKKYNELSRFEVGP
                     GDLIVSCRGTIGECHILPNDAPRGIIHPSLMMIKPKGSVNGKFLLHLLERILERQLDN
                     GSGVKMAIQAKELAKVKTIVPDRHLQDEYVLFVEQVDKLKFEVLKLLRELR"
     gene            1268..4537
                     /locus_tag="PLQ22_00875"
     CDS             1268..4537
                     /locus_tag="PLQ22_00875"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH58547.1"
                     /translation="MNFDYIKEINNLNDLYSFCKDAESFVLSRPDLSAGQSRKSLEYL
                     VKLVYQLRNGTVPQRSSLFELVSSEDFTNFINDSSMINALHYIRKVGNIAIHNEEVSQ
                     KEALLSLKQLHIFTGELLIKLDLIKSYPLFDESLLVKTTKTIPADKDVDLNQQLVSSL
                     KEKIDKHSTLGVGVNISEAQTRKIYIDLYLREAGWKVLDKDNIKLPEKAGVEIKVEGM
                     PNAQGIGFVDYVLYGSDGKPLAVVEAKKTSVSPIKGKEQALLYAKCLQKEYGYLPIVY
                     YTNGYQIWVIDQLGYPARQVFGFHNIKELEYMMQLRRRGAISDLTIKDEITDRPYQKM
                     AITSVAESFNNKRRKALLVMATGTGKTRTAISLVELLQRNRWIKNVLFLADRTALVTQ
                     AKRNFNQLLPNLTISVLSDRDKKPDLNARLVFSTYQTMINMIDGDDRVFGIGRFDLII
                     IDEAHRSIFNKYKAIFTYFDSLLVGLTATPRDEIERSTYSTFDLEEGLPTFNYDMDEA
                     VKDNYLVGYTVLDRTTKFLKQGVKYSDLSDEEKEEYEKTFLTPEGELPTELSGADFFK
                     KIYNDNTVDLVLQSLMNEGLRVNSGDMIGKTIIFAFNHNHAELIVKRFEKLYPELGPE
                     YCKLVDNYVTYAQNIIDTFTVREKLPQIAVSVDMLDTGIDVPDILNLVFFKRIYSKIK
                     FVQMIGRGTRKSNDIYGHLKHKDVFYIFDFCDNFSFFEINPEGRKVNQGLSVTQKVFQ
                     MKLDLLFELQKEENQRNEFHKTYYEMIKTELYQTIRNFNRDRILVRDNLPIVDKYSVE
                     AKWAYLSKYDIQEVKNKLTLLVDSDKDIESAKVFDLKVFYIMLSHLSDDIVAKRAIEQ
                     VVRISQTLLERLSIPQVAEKKDLLKEVTTQTYWNKANLETLEHLRNELRYLIQYITDE
                     VGIYSTDFKDELIDQGTKDVNILDFKTYEEKVIDYLLSNDANETIIKIKMLDKIDAND
                     LKELERILWQELGTKDDYFKVTKEENLAAFIRSIVGIEQAAINAKFSQYLDSNILTSK
                     QQEFVKSIINYVQQNGDITRQDLVEKVPFNNFDVVDLFEDNIDILVHVINNLHESILV
                     A"
     gene            4933..6045
                     /locus_tag="PLQ22_00880"
     CDS             4933..6045
                     /locus_tag="PLQ22_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH58548.1"
                     /translation="MEKEEVKKNSGVVLMDEELDEDGFPKTITLTKEVLEKLPPLAPE
                     LVEGILRKGHKMLISGSSKAGKSFLLMQLAIALAKGREWLGFKCKKSRVLYVNLEIDS
                     ASCINRFVEIYKRLGIEPEDDESLYVWNLRGKSMPLDKLMPTLIRRVNGRGFDVVIID
                     PIYKVITGDENKATDMAKFTNLFDKLCLKTGVAVIYSHHHSKGEQGFKRAMDRASGSG
                     VFARDPDSLLDIIDLILEDEFKQAYLDDQNMTAWRLESSLREFRNITPVNFWFDYPIH
                     IVDKEGLLAKNYASGDPRHNLTKSGKRKQTPESRKLEFDNAFDINLEEDGTCRVSTLA
                     EYLGVTDKTIKNRISEFSDEYINNRGVVSKKSEDGI"
     gene            6579..6962
                     /locus_tag="PLQ22_00885"
     CDS             6579..6962
                     /locus_tag="PLQ22_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58549.1"
                     /translation="MINYNYEWKTLDEILRNKKTKVCFRGYSETSLDEITDGASNKDY
                     KIAHELWLKLKEAFNKEAEIYDHELYQSKLGSIGPKEKREVLRTMWWDFVEGDKHGDY
                     RKIISIIEMLHDGEELESGIIYFGS"
     gene            6976..7194
                     /locus_tag="PLQ22_00890"
     CDS             6976..7194
                     /locus_tag="PLQ22_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58550.1"
                     /translation="MKPTKIYVLVAYAKDGRIFDLGFTEDIKLVREEINSESTKEQLQ
                     ELGINEVEKFVIRDNYFPTKYYPLEVSL"
     gene            7191..7760
                     /locus_tag="PLQ22_00895"
     CDS             7191..7760
                     /locus_tag="PLQ22_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3846 domain-containing protein"
                     /protein_id="HOH58551.1"
                     /translation="MISVGDKIKIISMKNEPQYSGKVGIVYAIDGIGQIHGTWGGLAL
                     VPKEDKFEIIKEKNCDICDKRFNEYVNNPSPIKADICCNECNEKIVVPLRIFQATKNA
                     NFALLIATDGTIKTVKPKEKYFNLKELQTNVEGLIEMYPTYWQDNFIVCNEEGLLKDM
                     EYNKLAKLILGVDLVGPVLVVPRPLLEED"
     gene            7764..8096
                     /locus_tag="PLQ22_00900"
     CDS             7764..8096
                     /locus_tag="PLQ22_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58552.1"
                     /translation="MSKIKDVQIEYERLRSLFSSVDPTKAELVDNLLNEAAFMRVQLE
                     NLQQQIRKYGAVQISSKGTQRQTEAAKYYTKLVNSYGNVIKTLNTIMGKNMIDDNDEF
                     DEFMKRLE"
     gene            8096..9733
                     /locus_tag="PLQ22_00905"
     CDS             8096..9733
                     /locus_tag="PLQ22_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HOH58553.1"
                     /translation="MNYLKEYYRQIQEGEILVGKELLTVLEGLIEDLNNPRYTFDEKP
                     GKIRIDFIETFCKHTKSPFNGEPFILELWEKAVLQVAYGFKMADTNLRRFNEVLLLVA
                     RKNGKTTFIAGIDLAEFFLSKGGVDIVCASNTTEQANILFEEINNMREQSKALSNEKR
                     SKKNIFHIYSPKTKNKIKKLSAQSRNKDGYNIEVGCIDEVHEMTDSKVYDAIKQSQST
                     KSEPLIFIITTEGNTVGGFLDNKLDYVRKMIKGEIQDERVLPWLYTQDSVDEIYQDKR
                     TWQKSNPSIGSIKTYSYLEDLMNKSKHDLGTRVTMLSKDFNIKQLEQGSWLSFDDLNN
                     NETFNIEDLKDSCAIGGVDLSSTTDLTAAVLLLMKGDKKFVITQFFMPSDVILKRKEE
                     DNAPYDIWVQKGLITVTDGSQNDFSLVTQWFMNMIRTYNIRPLWVGYDPWNSLYWIKE
                     MEELGFNMEKVRQGVYSLSEPMKQLEADLKNNKVVYNNNPILKWCLSNTQAKVDVNGN
                     IQPSKLNSRYKRIDGTVALIIAYAVLNRYKLDFENMI"
     gene            9748..10962
                     /locus_tag="PLQ22_00910"
     CDS             9748..10962
                     /locus_tag="PLQ22_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOH58554.1"
                     /translation="MAIFKRKKKQGSEQPFKFISELNIPQVSFGTNISKSDVVKIAID
                     RIASQCAKLKPRHIKNENDKTVTDKTGKLSFILKHKPNEVMTPYQFIYKVITKLFIDD
                     NSFIYPMFDDGGLKGLYPLNPIMVEPIVDSGNNYYLRFQFENKETFIIPYENIIHLKR
                     FYHDNDIFGGSGHKGDQEALLKAININENVLQGVENALRSSMQIKGLLKMNAMLNETD
                     KNKQLASFNEMLRESIKNKGSSIIPIDLKSEYIPLSVDPKLIDAETLEFLNDKILNYF
                     GVSAAIFSSNYSEDEFNSFYEQTIEPLAIQLSEAFSLGLLTDNEIKRGEQVVFYSERL
                     QYASWNTKVSAIEKLMGLGIMSLNESRALLGLEPVENGDKRLQSLNYVDALKANQYQV
                     GESENLNNEGND"
     gene            10946..11143
                     /locus_tag="PLQ22_00915"
     CDS             10946..11143
                     /locus_tag="PLQ22_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58555.1"
                     /translation="MKVTINGKVTKEAIASILAEQKEKTKIIDEYCKKEKLENFYYKD
                     AELEYEYIKEVTIKKKEVETR"
     gene            11140..11745
                     /locus_tag="PLQ22_00920"
     CDS             11140..11745
                     /locus_tag="PLQ22_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HOH58556.1"
                     /translation="MIKKETRLAEVELREEDEKMILEGYAILYNEETLIGTREYGFIE
                     SIDPNALSEDAIKDVPMKYNHMDSFLIIARTKNKSLELTSDEKGLKVRAELLDTQHNK
                     DIYKMVKSGLLEKMSFAFTVKEQEWDHEGEIPKRRITKIDRLYDVSIVDIPAYDNTSI
                     YARSLESMDLELKTMELEKRKIDEALLRKRIDLKIKIGGTK"
     gene            11745..12872
                     /locus_tag="PLQ22_00925"
     CDS             11745..12872
                     /locus_tag="PLQ22_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOH58557.1"
                     /translation="MNLEKRKQEIKVRLNEIKGLTGVEATLEVLEDLEKEVDELKEEE
                     ETIDRKLAIQRKAVINPIQIERTDQVNKEELEKRGAMLKEARVIQVSSEEILLPEHTA
                     PNIAAYPFAQVSSLVDRVKVVNLTGGETYKKSFIKGNGIAGLTAEGAAYSETEPDFGY
                     LTITKVKVTAYTEITEELEKLPSLPYQAEVIKNINIALKKKISEQILKGPGTSNTFTG
                     IFSDQAVALADSTPLEISAITDSTLDDIVFAYGGDEEIEGGAYLILNKNDLRAFAGLR
                     TPEGRKVHTIDYINSTIDGIPYIINSHCKALTDTNTSEGEYVMAYGGLHNYEVPIFSP
                     VEIGKSTDYKFKDGIISYKASVFTGGNVVGYNGFLRIKKKA"
     gene            12886..13176
                     /locus_tag="PLQ22_00930"
     CDS             12886..13176
                     /locus_tag="PLQ22_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HOH58558.1"
                     /translation="MGLLENVKKSLLIPLEETYADDELNSYIEACIALILSTGVDPEN
                     IEDNPLTKSLVLIYCKTFFGFKTDGSVKELPRSFDMLLLQLALSKGDTNVPK"
     gene            13163..13498
                     /locus_tag="PLQ22_00935"
     CDS             13163..13498
                     /locus_tag="PLQ22_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58559.1"
                     /translation="MFPSSPNIKLELLKLKSIKDNIGNNKLSLISKKQVIGIKKQVTS
                     KEYYESKKQEYKIDLSLKIQSFLYDGSKYALVDGVIYSVERTYLAGQFLELYLVESKL
                     KVSDIDGYA"
     gene            13485..13862
                     /locus_tag="PLQ22_00940"
     CDS             13485..13862
                     /locus_tag="PLQ22_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HOH58560.1"
                     /translation="MATLDNLAELIMDEVSSYTDEVIKALEAKLDETADKILEYIKSN
                     APRSGQKDGLADDFVKADVGSGVNKTIVIYGKEKGMLVHLIEFGFMHRSGKYVNPRPF
                     LRPAYDTFTPKMLEDIKVIIRGK"
     gene            13852..14199
                     /locus_tag="PLQ22_00945"
     CDS             13852..14199
                     /locus_tag="PLQ22_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58561.1"
                     /translation="MVNNLEYIYTILNEVLPNNVYYAVSVKDNIELPIIVYQELNKRG
                     KTYADDSYLLKELTIQITLITPNKNIQLEKMLENKLKLYDIEFQMISEFYIAENGLYR
                     IYEIKMEESKYEQ"
     gene            14189..14758
                     /locus_tag="PLQ22_00950"
     CDS             14189..14758
                     /locus_tag="PLQ22_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58562.1"
                     /translation="MSNKVTFGLKNVHYAVATPCEDDTWTYGEPKKLTEAQELTAEVI
                     AGKTDVYADDRILATLVSNSGSNITLKLTEIDDYVKVAVLGYEKDTNNNLIEVVNYRN
                     KTFALGYEIQGDAKARRIWYFLCTASPVSDATKTKAESIEPNSVTLSITARSIEVGNL
                     SVIRTIAKFGDANYNNFFTSGPSVGTIGT"
     gene            14760..>14992
                     /locus_tag="PLQ22_00955"
     CDS             14760..>14992
                     /locus_tag="PLQ22_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58563.1"
                     /translation="MEKTVKLNGEDIRLKSSLFTIIEYRSTFGTELFSDITKLESSEK
                     EGNVSEVLEVIFKIVYVLHKPFTKKSYEEFLQG"
BASE COUNT         5897 a         1895 c         2730 g         4470 t
ORIGIN      
        1 aaattgaagt tcttaaaaat gagcttaaat cacttctaaa tttagatttg taggggtgag
       61 cttaatgaaa aatagagtaa aaatccctaa attattattt tttcaagaag gaccaggagt
      121 tagaaattat caatatacaa ctgagggagt aaaactccta aatgtagcta atttagttaa
      181 tgggaattta gatttatcaa catcagaaag atatatatct gaagaagaag catatggaaa
      241 atacaaacac tttttatgtg atgaaggaga tttaattatt gcaagttcag gaatcaaagt
      301 agattatttt gataaaaaaa tgggatttgt aaaaccggaa cacttaccat tatgcatgaa
      361 cactagtaca attagattta aatctcttga tagagaagtt ttagatataa attatttcat
      421 gtatttttta aaatctcaag ctttcaagaa acagttagcc agacaaataa ctggatctgc
      481 acaattgaat tttggaccat cacaccttag taaaatgagt ttctctttaa ttgatatgga
      541 agatcaaatc aatattgtta aacaattagg aaaaatagaa ttaattattg aaatgaaaac
      601 taaacaaatt caagagtatg accaacttat caaatcccga tttgttgaga tgtttggaga
      661 catttttaca aatgaaaaaa ggtatagaat tggagtattt aatgatttcg taacacaaat
      721 gaacattgga cctttcggaa gtgctttaaa gaatgattat tttgtgtcaa aagatgatgg
      781 atattgtatg gtttatgagc aaaaacatgc catacgtaaa aatatttatg ttgatagaag
      841 atatgtggat ctaaaaaaat ataatgaatt aagtagattt gaagtgggac cgggagattt
      901 aatcgtaagt tgtagaggaa caataggaga atgccatata ttgccaaatg atgctcctcg
      961 agggataatt catccctcgc taatgatgat taagccgaag ggttctgtaa atggtaagtt
     1021 tctgctgcac cttttagaaa gaatactgga acgacaatta gataatgggt caggtgtgaa
     1081 aatggctatt caagctaaag aactcgcaaa agtgaaaact attgtacctg atagacattt
     1141 gcaagatgaa tatgttcttt ttgttgaaca agtcgacaaa ttgaaatttg aagttttaaa
     1201 attactgaga gaactcagat aaaatagatt tataaagata aaaattatta aaaaaggaag
     1261 tgacgccatg aattttgatt atattaaaga aataaataat cttaatgact tatattcttt
     1321 ctgtaaagat gcagaatctt ttgttttgtc gcgtcctgat cttagtgcag gtcaatctag
     1381 aaaatcactt gaatatttag tcaaactagt ttatcaacta agaaacggta ccgttccaca
     1441 aagaagttct ttatttgaac ttgtatcatc agaagatttt acaaacttta ttaatgactc
     1501 ttcaatgata aatgcgcttc actatataag aaaagttggc aatattgcga ttcataatga
     1561 agaagttagt caaaaagaag ctttgttatc tttaaaacaa ctgcacatat ttacaggcga
     1621 gttattaatt aaactcgatt taatcaagtc atatccttta tttgatgaat cactattagt
     1681 aaaaacaact aaaacaattc ctgctgataa agatgttgat ttaaatcaac aactcgtaag
     1741 ttctctaaaa gaaaaaatag acaaacattc aactttaggt gttggtgtta atataagcga
     1801 agctcaaaca cgcaaaattt atatcgatct atatttaaga gaggctggat ggaaggtttt
     1861 agataaagat aatattaaac ttccagaaaa agctggagtt gaaattaaag tagagggtat
     1921 gccaaatgct caaggcattg gttttgttga ttatgtttta tatggtagtg atggaaaacc
     1981 tttggcagtt gtagaagcta agaaaactag tgtatcaccg attaaaggga aagaacaagc
     2041 attactgtat gctaaatgtc tacaaaaaga atatggatat cttccaattg tttattacac
     2101 aaacggttat caaatttggg ttattgacca attagggtat ccagcaagac aagtctttgg
     2161 tttccataat attaaagagc ttgaatatat gatgcagctt agacgaagag gagcaatttc
     2221 tgatttaaca attaaagatg aaattaccga tagaccatac caaaaaatgg ctataacaag
     2281 cgttgctgaa tcatttaaca ataaaagaag aaaagcctta cttgttatgg caacagggac
     2341 aggcaaaaca agaactgcta tctcgttagt tgaattacta caaagaaata gatggataaa
     2401 aaatgtttta tttttagctg atagaacagc tttagttact caagcaaaga gaaactttaa
     2461 ccaattatta ccaaatttaa caatctcagt tttatctgat agagataaaa aaccagattt
     2521 aaatgcaaga ttggtatttt caacatatca aacaatgatt aatatgatag atggtgatga
     2581 tagagttttt ggtattggta ggtttgattt aattattata gatgaagcac atagatctat
     2641 ttttaataaa tataaagcaa tattcactta ttttgattca ctacttgtag gtttaacagc
     2701 aacaccaaga gatgagatag aaagatcaac ttattcaacg tttgatttag aagaagggtt
     2761 acctactttt aattatgata tggatgaagc tgttaaagat aactatttag taggatatac
     2821 tgttttagat cgaacaacta aattcttaaa acaaggtgtt aaatatagtg atttatcaga
     2881 tgaagaaaaa gaagagtatg aaaaaacatt cttaacacct gaaggtgaac tacctacaga
     2941 attaagtggt gctgatttct ttaagaaaat atataacgat aatactgttg atttagtatt
     3001 gcaatcttta atgaatgaag gattaagggt taatagtggt gatatgattg gtaaaacaat
     3061 tatatttgct tttaatcata atcatgccga acttattgtt aaaagatttg aaaaattata
     3121 tccagaactt gggccagagt actgcaagct cgttgataac tatgtaactt acgctcaaaa
     3181 tattattgat acttttacgg taagagaaaa attgccacaa attgcggtgt ccgttgatat
     3241 gcttgataca ggtattgatg ttcctgatat tttaaattta gtgtttttca aacgtatata
     3301 ttcaaaaatt aaatttgtac aaatgattgg tagaggaaca cgcaagtcaa atgatattta
     3361 tggacactta aaacacaaag acgtatttta tatttttgat ttttgtgata actttagttt
     3421 ctttgaaata aatcctgaag gtagaaaagt taatcaagga ttatcagtaa cacaaaaagt
     3481 attccaaatg aaactagatt tattgtttga actacaaaaa gaagagaatc aaagaaatga
     3541 gttccataaa acatattatg aaatgattaa aacagagtta tatcaaacaa taagaaactt
     3601 taatagagac cgtattttag taagagataa cttaccaata gtagataaat attctgttga
     3661 agccaaatgg gcttatctat ccaaatatga tattcaagaa gttaaaaaca agttaacctt
     3721 attagttgat agtgataaag atatcgaatc agctaaagtg tttgacctaa aagtatttta
     3781 cattatgctt tcacatttat cagatgatat agttgctaaa agagcaattg agcaagtagt
     3841 aaggatatca caaacacttc ttgagagatt aagtatccca caagttgcag agaagaaaga
     3901 cttattaaaa gaagttacca cacaaactta ttggaataaa gcaaatcttg aaactcttga
     3961 acacttaaga aatgaattaa gatatttaat tcaatatata actgatgaag taggcattta
     4021 ttctaccgat tttaaagatg aacttattga tcaaggaact aaagatgtta atattttaga
     4081 ttttaaaaca tatgaagaaa aggttattga ctatttactg tctaatgatg caaatgaaac
     4141 aattattaaa attaagatgt tagataaaat agatgccaat gatctaaaag aactcgaacg
     4201 aattttatgg caagaactag gtacaaaaga tgattatttc aaagtgacaa aagaagaaaa
     4261 cttagcagca tttataagaa gtattgttgg aatagaacaa gcggcaataa atgcgaaatt
     4321 tagccaatac ttagattcaa acatattaac ttctaaacaa caagagttcg taaaaagcat
     4381 tattaattat gttcaacaaa atggagatat tactagacaa gatttagttg aaaaagtacc
     4441 gtttaataat tttgatgttg tggatttatt tgaagacaat attgatattt tagtacatgt
     4501 cattaataat ttacatgaaa gcattttagt cgcataaaag ttattaacga aggtttataa
     4561 aataaaacaa ttaaataaat gtatatagcg gtttagctca aaatttgagt tagatcgctt
     4621 tttttatttt aaaaattaat aaaattcact aaatcttggc aaaacaacaa tcttcatgcc
     4681 attaaccatt gaggaagtgg tgaagttatc tatttaagtt gttaaaacac ataaaattaa
     4741 caaaaattca aaattaaccg gcaaaacagt accctgtttg ccattaacca ttgaagaggt
     4801 agttggatta tctgttttag ttactaaaaa tgtgaaaata taggaatttt ctaaaaaaac
     4861 taaggaattt cactacttgc ttgccattaa cctatgaaag gaaaaatatt ttatcaaaag
     4921 gaggaaagaa aaatggaaaa agaagaagta aagaaaaata gtggggttgt tttaatggat
     4981 gaggagctag atgaagatgg atttccaaaa actataactt taacaaaaga agtattggaa
     5041 aagttaccac cattagcacc agagttagta gagggtattt taaggaaggg acacaagatg
     5101 ttgatatctg gttcatctaa ggccggtaag agctttttgt tgatgcaact tgctattgcg
     5161 cttgctaaag gtagagaatg gttaggtttt aaatgtaaaa aatcaagagt tctatatgtc
     5221 aatctggaaa tagactcagc aagttgtatt aataggtttg ttgaaattta taagagacta
     5281 ggcatagaac ctgaagacga tgaatctttg tatgtttgga atttaagagg taaatcaatg
     5341 cctcttgata agctaatgcc aaccttaatc aggagagtta atggtcgtgg ttttgatgta
     5401 gttattattg atcctattta taaagtgata actggcgatg aaaacaaagc aaccgacatg
     5461 gcaaagttta ctaatttatt cgataagctt tgtttaaaaa caggtgtagc ggttatttat
     5521 tctcaccatc attccaaggg tgaacaagga tttaaaagag caatggatcg tgcatctggt
     5581 tcaggagtgt tcgcaagaga tccagattca ctgcttgata tcattgatct tattttagag
     5641 gatgagttta aacaagcata tttagacgat cagaatatga ctgcttggcg tcttgaaagt
     5701 agtctaagag agtttagaaa tatcacacca gttaactttt ggtttgatta tccaatccat
     5761 atagtagata aagaaggatt acttgccaaa aactatgctt caggtgatcc gagacataac
     5821 ttaaccaaga gtggaaaaag gaaacaaaca ccggaatcta gaaagttaga atttgataat
     5881 gcctttgata ttaatttaga agaagatgga acttgtagag tatctacttt ggctgaatat
     5941 ttaggcgtaa cagataaaac gattaaaaat agaattagtg aatttagtga tgaatatata
     6001 aataatcgtg gtgttgtttc aaaaaaaagt gaagatggaa tatagggaaa acatctgctt
     6061 cactcactaa aaattcggaa agcgaaaata gggataaatg ccctttattc accaaaacaa
     6121 gaaaagtgga aaataggcct tatatatatg aagttccttc cactgctgac gcatgtcgtt
     6181 tgtaggataa gggcatcgta gtaactgccc tatcccaaac aaatgcatca ccagtcagta
     6241 ctgccttttt tctaaaccaa aaatcttaaa aactctaaaa agtggttaac tattacaaca
     6301 aatttatagg atttttcaaa aattttaagt accatacccc cctcataagc ttttaacaag
     6361 tgacgatggg taccgcatgg gggggcaatt aaaaaacacg aggcggtatt tttgaaaatc
     6421 tgattttaca ttttaagaat ggttaaaaat aagtcactaa tggtggcttt ttcttttgta
     6481 aaaagcatca aaaattataa taaattatag ttttgaattg agttgatata gaagtcctta
     6541 tacggtaata tgtgtatgaa aaagaggagg tagtaaaaat gattaattat aattatgaat
     6601 ggaaaacact agatgaaatt ttaagaaata aaaagactaa agtttgcttt agaggttaca
     6661 gtgaaacttc actcgatgag attactgatg gcgcaagcaa taaggattat aagatagcac
     6721 acgagttatg gttaaaatta aaagaagcct ttaataaaga agcggaaatt tatgaccatg
     6781 aactttatca aagtaaactt ggttcaatag gaccaaaaga aaaaagagaa gttttaagaa
     6841 caatgtggtg ggacttcgtt gaaggtgata aacatggtga ttatagaaaa atcataagca
     6901 tcattgaaat gttacatgat ggcgaagagt tagaaagtgg aattatctac tttggaagtt
     6961 aagggtgtaa aaaaaatgaa accaacaaaa atttatgtat tagttgcata tgcaaaagat
     7021 ggtagaatat ttgatttagg atttactgaa gatattaaat tagtaagaga agaaataaac
     7081 tctgaatcaa caaaagaaca attacaagaa ttaggaataa atgaagttga aaaatttgta
     7141 attagagaca actacttccc aactaaatac tatccattag aggtaagttt atgattagcg
     7201 ttggtgataa gataaaaatc atctcaatga aaaatgaacc acaatatagt ggtaaagttg
     7261 gaatagtata tgcaatcgat ggaataggtc aaatacatgg tacctggggt ggattggcac
     7321 ttgtaccaaa agaagataag tttgaaatta tcaaagaaaa gaattgtgat atttgcgata
     7381 aaagatttaa tgaatatgta aacaatccat caccaattaa agctgatatt tgttgtaatg
     7441 aatgtaatga aaaaatagtt gtaccactta gaatattcca agcaactaaa aatgcaaact
     7501 ttgccttatt aatagcgact gatggaacca ttaaaacagt aaaaccaaaa gaaaaatatt
     7561 tcaatctaaa agaactacaa acgaatgtgg aagggttaat tgaaatgtat ccgacatatt
     7621 ggcaagacaa ttttattgta tgtaatgaag agggattatt gaaagatatg gaatacaaca
     7681 agttagcaaa attgatactt ggggttgatt tggttggtcc ggtattggta gttcctagac
     7741 ccttactaga ggaggattag ttaatgagca aaattaaaga tgtacaaatt gagtatgagc
     7801 gactaaggtc gcttttttca tcagttgatc caaccaaagc agaactagtg gataatcttc
     7861 taaacgaagc agcatttatg agagtacaat tagaaaactt gcagcaacaa ataagaaagt
     7921 atggtgcagt tcaaatttca tcaaagggaa ctcaaagaca aactgaagct gcaaaatact
     7981 atacaaagtt agttaatagc tatggaaatg ttattaaaac tcttaataca atcatgggta
     8041 aaaacatgat agatgacaat gatgaatttg acgaattcat gaaaaggtta gaatagtgaa
     8101 ttatttaaaa gagtattata ggcaaattca agaaggcgag atattagttg gtaaagaact
     8161 actcacagtt ttagaaggtt taattgagga tttaaataat cctagatata catttgatga
     8221 aaaaccaggc aaaataagaa ttgattttat tgagacattc tgcaaacaca ctaaaagtcc
     8281 atttaatggt gagccattta tattagaact ttgggaaaaa gcagtccttc aggtagccta
     8341 tggatttaaa atggctgata caaacttaag aaggtttaat gaggttttat tattagtagc
     8401 aagaaagaat ggtaaaacta cctttattgc aggtattgac ttagctgagt ttttcttatc
     8461 aaaaggtgga gttgatattg tatgtgcatc taacacaaca gaacaagcga acatcttatt
     8521 tgaagaaatt aacaatatga gagaacaatc aaaagctcta tcgaatgaga aaagaagcaa
     8581 aaaaaacatt tttcatattt actcaccaaa gaccaaaaac aagataaaaa agttatctgc
     8641 tcaatcaaga aataaggatg gctataacat cgaggttggc tgtattgatg aagtgcatga
     8701 aatgacagat agtaaagttt atgatgcgat taaacaaagc caatcaacaa aatcagaacc
     8761 acttatattt atcataacca ctgaaggaaa cactgtcggt ggttttttag ataataagct
     8821 ggattatgtt aggaaaatga tcaaaggtga aatccaagat gaaagagtgc taccttggtt
     8881 atatacacaa gattcagttg atgaaatata tcaagataaa agaacatggc aaaaatctaa
     8941 cccaagcata ggttcaatta agacatattc ctacttggaa gatttaatga acaaatcaaa
     9001 gcacgatctt ggaacaagag taacaatgct atcaaaggat tttaatatta aacaattaga
     9061 acaaggctca tggttgagtt ttgatgatct taataataat gaaaccttta atattgaaga
     9121 tttaaaagat agttgtgcaa ttggtggtgt tgacctttca agcacaacgg atttaacggc
     9181 agctgttcta ttactaatga aaggtgataa aaagtttgtt attactcaat tttttatgcc
     9241 aagtgatgtc attctaaagc gtaaagagga ggacaatgca ccttatgata tttgggtgca
     9301 aaaaggtctg attacagtaa cggatggaag ccagaacgat ttttcacttg ttactcagtg
     9361 gtttatgaat atgataagga catataacat tagaccactt tgggtaggat acgatccatg
     9421 gaatagtctt tattggatta aagaaatgga ggagttaggt tttaatatgg aaaaagtcag
     9481 acaaggagtt tattctttat cagaaccaat gaaacaactt gaagctgatt taaagaataa
     9541 caaagttgtc tataacaaca atccaattct aaaatggtgt ttatcaaata cacaagcaaa
     9601 agtagatgtt aatggaaaca tccaaccatc aaaattaaac tcaagatata aaagaattga
     9661 tggaacagta gcacttataa ttgcttatgc tgttttaaat agatataagt tggattttga
     9721 aaacatgatt taatcaagga ggtcgcaatg gccatattta aaagaaagaa aaaacaaggc
     9781 tcagaacagc ccttcaaatt tataagcgaa cttaatattc cacaagtttc atttggaaca
     9841 aacatctcaa aatctgatgt agtaaagatt gccattgata gaatagcaag ccaatgcgcc
     9901 aaactaaaac ctagacatat taaaaacgaa aacgataaga cagtgactga taaaaccgga
     9961 aagctgtctt ttattttaaa gcacaagcca aatgaggtaa tgacacctta tcagtttatc
    10021 tataaagtaa ttacaaaact tttcatagat gacaattcat ttatttatcc aatgtttgat
    10081 gacggtgggc ttaaaggttt atatccactt aatccaatta tggtagaacc aattgttgat
    10141 agtggtaata attactattt aagatttcag tttgaaaaca aagaaacatt tattattcct
    10201 tatgagaaca ttattcattt aaaaagattt tatcacgata acgatatctt tggtggaagt
    10261 ggacataagg gagaccaaga agcattactt aaagcaatca acattaatga aaatgtgctt
    10321 caaggagtag aaaacgcact aagaagttca atgcagatta aagggcttct taagatgaat
    10381 gcaatgttaa atgaaacaga taagaataaa caactagcct catttaatga aatgttaaga
    10441 gaatcaataa aaaataaagg aagctcaata attccgattg atttaaaaag tgagtatata
    10501 ccacttagtg tagatccaaa actaattgat gcagaaacac tagaattctt aaatgataag
    10561 atattaaatt atttcggtgt gtcagctgca atctttagtt caaattatag tgaagatgag
    10621 tttaattcat tttatgaaca aaccatagag cccttagcca ttcaattgtc tgaggctttt
    10681 tctttgggac ttcttactga taatgaaatc aaaaggggtg agcaagttgt tttttatagt
    10741 gaaagactac agtatgcatc ctggaacaca aaggtaagtg caattgaaaa gttaatggga
    10801 cttggaatta tgagcctaaa tgaatcaaga gctctacttg gtttagagcc agttgaaaat
    10861 ggtgataaaa gattacaatc actaaattat gtggatgctt taaaagctaa tcaatatcaa
    10921 gttggagaaa gtgagaattt aaataatgaa ggtaacgatt aatggcaagg taacaaaaga
    10981 agcaatagca tcaattttag cagaacaaaa agagaaaact aaaattattg atgagtattg
    11041 taaaaaagaa aagcttgaga acttttacta taaagatgct gagcttgagt atgaatatat
    11101 aaaagaagta acaattaaga aaaaagaggt ggaaaccaga tgattaaaaa agaaacaaga
    11161 cttgcagaag ttgaacttag agaagaagat gaaaagatga ttttggaagg ttatgcgatt
    11221 ctttataatg aggaaacctt aatcggaaca agagagtatg gtttcattga aagcattgat
    11281 ccaaatgcac taagtgaaga tgcgataaaa gatgtaccaa tgaagtataa ccatatggac
    11341 tcattcttaa tcatcgcaag aaccaaaaac aaatctttag aacttacatc agatgaaaaa
    11401 ggactaaagg taagagctga actcttagat actcagcaca acaaagatat ttataagatg
    11461 gtaaaatcag gacttttaga aaagatgagt tttgctttta cagttaaaga acaagagtgg
    11521 gatcatgaag gtgaaattcc aaaaagacga atcaccaaaa ttgatagact ctatgatgta
    11581 tcaatcgttg atattccagc ttatgacaac acttcaattt atgctcgttc tttagagtct
    11641 atggacttag aactaaagac tatggaatta gaaaagagaa aaattgatga ggcgttatta
    11701 aggaaaagaa tagatttaaa aataaaaatt ggaggaacta aataatgaat ttagaaaaaa
    11761 gaaaacaaga aatcaaggtc agattaaatg agatcaaagg cctaacaggt gttgaagcaa
    11821 cacttgaagt attagaggac ttagaaaaag aagtcgatga attaaaagaa gaagaggaaa
    11881 caatcgatag aaagcttgct attcaaagaa aagctgtcat caatccaatt caaattgaaa
    11941 gaaccgatca agttaataaa gaggaacttg aaaaacgtgg tgcaatgcta aaagaggcaa
    12001 gagtaattca ggtatcaagt gaagagattt tacttcctga acatactgct ccaaacattg
    12061 cagcttatcc attcgctcaa gtttcaagtt tagttgatcg tgttaaagtt gtaaatttaa
    12121 ctggcggtga gacttataag aaatcattta ttaaaggtaa tggcattgct ggtttaacag
    12181 ctgaaggtgc tgcttatagt gaaacagaac cagactttgg ttatttaaca attactaaag
    12241 ttaaagttac tgcatacact gagattactg aagagttaga aaaactacca agcttacctt
    12301 atcaagcaga agttattaaa aatattaaca ttgctttaaa gaaaaagata agtgaacaaa
    12361 tcttaaaagg tccaggaaca tcaaatacat tcactggtat ctttagtgac caagcagtag
    12421 cacttgcaga tagtacccca cttgaaatct cagcaatcac tgactcaaca ttagatgata
    12481 ttgtctttgc ttatggtggc gatgaagaga tcgaaggcgg agcttatttg atcttaaata
    12541 aaaatgattt aagagcattt gcaggactta gaactccaga gggtagaaag gtccatacaa
    12601 tcgactatat taatagcaca attgatggta ttccatatat tattaattcg cattgtaaag
    12661 ccttaactga cacaaacact agtgaaggtg aatatgtaat ggcttatggt ggattacata
    12721 actatgaagt acctattttc tcaccggttg agattggcaa atcaactgat tacaaattca
    12781 aagatggcat tatttcatac aaagcatcag ttttcactgg tggtaacgtt gtaggttata
    12841 acggattctt aagaattaag aaaaaagcct aagaggtgtt tataaatggg actacttgag
    12901 aatgtaaaaa aatcactgct tattccttta gaggaaactt acgcagatga tgaactaaat
    12961 agttacatag aagcgtgcat tgctttaatt ttatcaacag gcgtggatcc agagaatatt
    13021 gaagataacc ccctaacaaa atctttagta ttaatttact gtaaaacatt ctttgggttt
    13081 aaaacagatg gttcagttaa agagttaccg aggagttttg atatgctttt gttacaactt
    13141 gcactatcta agggtgatac taatgttccc aagtagtcct aatataaagc ttgagttgtt
    13201 aaaacttaaa tctattaaag ataacatagg aaacaataag ctctcactaa taagtaaaaa
    13261 acaagttata ggaattaaaa agcaagtaac ctcaaaagaa tactacgaaa gtaagaagca
    13321 agaatacaag atagatttat ctcttaaaat tcaaagcttc ttatatgatg gtagtaagta
    13381 tgctttagtt gatggtgtta tttattcagt tgaaagaacc tatcttgcag ggcagttttt
    13441 agaactatat ttagttgaat caaaactaaa ggtaagtgat atagatggct acgcttgata
    13501 acctagctga attgataatg gatgaagtta gtagttatac tgatgaagtt ataaaagcac
    13561 ttgaagcaaa gcttgatgag acagctgaca aaatacttga atacattaaa agtaatgcac
    13621 caagaagcgg acaaaaggat ggtcttgctg atgattttgt taaagcagat gttggtagtg
    13681 gagttaataa aacgattgtt atatatggaa aagaaaaagg tatgcttgtc catttgattg
    13741 aatttggatt tatgcataga agcggaaagt atgtaaatcc tagaccattt ctaagaccag
    13801 cttatgatac gtttactcct aaaatgttag aggatataaa ggtgatcata cgtggtaaat
    13861 aatcttgaat atatttatac aatcttaaat gaagtactac ctaataatgt ttattatgct
    13921 gtttcagtaa aagacaacat tgaattacca attattgttt atcaggaact taacaaaaga
    13981 ggtaaaactt atgctgacga ttcataccta ttaaaagaat taacaattca aataacttta
    14041 ataacaccta acaaaaacat tcaattagaa aaaatgctag aaaacaaact taaattatat
    14101 gacattgagt ttcaaatgat tagtgagttt tacatagcag aaaatggatt atatcgtatt
    14161 tatgaaataa aaatggagga atcaaaatat gagcaataaa gtaacattcg gacttaaaaa
    14221 tgtgcactat gctgtagcaa ctccttgtga agatgacact tggacatatg gtgaaccaaa
    14281 gaagctaaca gaagcgcaag aattaacagc tgaagttatc gctggcaaaa ctgatgttta
    14341 tgctgatgat agaattttag cgacacttgt ttctaactca ggatccaaca ttactctaaa
    14401 attaactgaa attgatgatt atgttaaagt ggcagtgtta ggatatgaaa aagatacaaa
    14461 caacaactta attgaagtag taaattatcg taataagact tttgcacttg gatatgaaat
    14521 tcaaggagat gcaaaagcaa gacgcatttg gtatttctta tgtactgcct caccagtaag
    14581 tgatgcaaca aaaactaaag cagaaagcat cgaaccaaac tctgtgacac ttagtattac
    14641 tgcaagatca attgaagtag gtaatttatc ggtaattaga acaattgcta aatttggaga
    14701 tgctaactat aataacttct ttacaagtgg tccttctgtt ggaactatag gtacttaata
    14761 tggaaaagac agttaaatta aatggcgagg atattagatt aaagtcctcg ctttttacta
    14821 ttatcgaata tcgcagcaca tttggtactg agttatttag tgatataaca aagcttgaaa
    14881 gtagtgaaaa agaaggaaat gtatcagaag ttttagaagt gatatttaaa attgtttatg
    14941 tccttcacaa acctttcact aagaaaagct atgaagagtt tttacagggc at
//
LOCUS       DAOSGS010000009        14622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_3382_length_14622_cov_6.327315, whole genome shotgun sequence.
ACCESSION   DAOSGS010000009 DAOSGS010000000
VERSION     DAOSGS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3382_length_14622_cov_6.327315"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLQ22_00960"
     CDS             complement(<1..238)
                     /locus_tag="PLQ22_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH58564.1"
                     /translation="MEYRKMNKLNISPSLLGFGAMRLKTKANGDIDYELGDKLIDKAY
                     KNGVNYFDTAATYTDGLNEKFLGQSIKRYPRESYY"
     gene            complement(324..3035)
                     /locus_tag="PLQ22_00965"
     CDS             complement(324..3035)
                     /locus_tag="PLQ22_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58565.1"
                     /translation="MKKLRRLGLVLLFALMAFVVVGCSCVPTEEDPTMGYLEEAQGKI
                     VFLGDANNLKQDEQLQDTVIVQDADGNRITVKVAWSISKGSEYLKVEKTAEGKTMLRV
                     TRPEGSEQGEATLVATLTIEGKDGSKTREFTIYIAPAPRALTVAELFEAELNQDVQGE
                     GIVTHSWEDSSGVLAFFKDETGSFFIYRAKSDHPERVKPGAKVSVSGTRKNHYNCQQI
                     AGDTPRITVLEDAPAAGYVYGDLGPAKNAAEVEAMGQDIHLPGTFVRLTGKAVQKTEG
                     QYTNSYLDDLASGKSISIYYGGHPQGLADFNAKLDKYVELDAVLYSYSGGWRVSVIPG
                     TVVEVAAPELTDEEKVAAAIGNLNTTFPENYAVYKNVKLPTTSALDEAIAIAWESDNA
                     AVLSKTGAYSRPDATTTVKLTATITLREVTETVELTFLAPVHEVSTIAQALAMDPANK
                     PVVHVQATVTGVLKDGIVVEDSTGAILLYGSSYGGAKYGDVVDVIGKRDAYKGLQQLA
                     IITVDVMTPGEGYDHASKAVEKTVAELCAIPATDLTNIGKIYKVSGKLAVAGRYNNYY
                     IAAADDEAEGAEGKLYIYYNNMPETLNQFDMPQVGRKIDSALLVVMGHHDDDKWRYVL
                     VPESVVFTALNDAETVALAKKYLALELPAGKEVDKDLEFFAEAPIDDKMVQVAWATDK
                     PEIIAADGKVNAPAEATEVKITVTITFGEATDTYEVTVSVAASTVVKKIDFGTTAKTG
                     YAQTTIEFENGDGEAMSINTLRAQTNSSNYDPHKNSGCMLVLSTKKSGQESWAEFDLT
                     SYEAAEIKFKIAPWNTKKYETIMGLTDSVLKIQVKQDEEWVDVKLTGREDANFLPDLL
                     KDEYVEISSNFTPGLYRIYYNTPSATYDDGNTEYALVVDDITLHKAP"
     gene            complement(3060..4538)
                     /locus_tag="PLQ22_00970"
     CDS             complement(3060..4538)
                     /locus_tag="PLQ22_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH58566.1"
                     /translation="MINKFRRITFSLVFLLFGFLLVGCREAETIRLNVEKTDLLVGET
                     FQLEATVFPENSKYKWESSNEKVVTVKNGLVTAIGPGRVTVRAISGKKTAAATFEIYG
                     HLDTSYTDNLKLEKSFEGKSFLTNGIGEVELGQLVDGDTAHFRNKGERGTFTLRFLYI
                     NTPESTGRIDPWGKAASSFVGNILTNAHQIVLETADGKSAQLDATGKRYLGLVWYRNS
                     ASEEFRLLNLEIVENAYSNYTGSNGDNDNYVDTFISAASKTAATRRRVFGEFDPSFNY
                     TGEIVEVSIAEIRKNYDDYDDGTKLLIEAQIMRITGDNLYLEDLEATDFDDEIKKAGI
                     YIFSGYGSGLGALKVGTIVRFQCQVGISDIYGLQLTNPSQVRILANEDGAEVEYTEVP
                     ADFTSLEDYEGYVVIVRNVTVSKVSSPNEEGAYTIYCKTETGAEINFRVDGGAYPKLD
                     YNSIVVGDKYIGIGGVSKFHDTYQVMIGNQTSGINDFVNVSK"
     gene            complement(4535..6013)
                     /locus_tag="PLQ22_00975"
     CDS             complement(4535..6013)
                     /locus_tag="PLQ22_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH58567.1"
                     /translation="MRRFLLVLVAIFSLILVAGCSCQVDKGDKVFEDLTEVVKTGEKD
                     GVAIEKVQITVSVTSGVVSDSLTPLIPLFEAEYPHVGITLKTISGGYTELRKTNILEI
                     QSYQAPTVTLGYPDHFAEYYSAGAMINLSNFIAGPNGYTQAELDDFIPSYLAEGKGFD
                     DANPNDYYSLPFNKSTEVLVYNKTILEAAMAKDATIKVPKTWAEVKSVSDKLLALGAA
                     GELDGIITVAAGEYKISEHINRGTFYTFAYDSSANAFITFTRQVKSTYTERANNEQGY
                     LAFDTPANVANLKYFQDENTAKRFAVAETFGSNYASDAFKRGQVLFTVGSSAGIKYNE
                     PEGNKFEIGISTVPYFTEDAKYVIQQGTNIAMLAQSTNLERAAAWLFIKFLLQPEVTA
                     KFAMASGGYLPVRLSAYETEAYQEYLDNPPLEKRSYSKAANLALQYRAQGYQFFVDPA
                     FVGSSKIRDEVGSALVSVIVNKAEVKKRLEDAYATLGKAYQK"
     gene            complement(6048..7187)
                     /locus_tag="PLQ22_00980"
     CDS             complement(6048..7187)
                     /locus_tag="PLQ22_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH58568.1"
                     /translation="MGFKEILLILLGLAALFGIGYLIVYLVSKHKQKVKAEQLALKSG
                     ADLSELERAKEIKRKEKEAYVPEDQLKAFRIKRTILKVITYIFLILVALFILVPFYWM
                     VITSFKSAEEIAATRPSLIPDKITFEAYKNIFRIEVNPYNGTLRRGVPFLYMFGNTIL
                     VGFFTTIFTVISVVFSAFAFSRLRFKGSDLLFTILLSTMMVPGEVFIITNYGTVSRLG
                     WVGAPLNAETGRIVGNYLAMILPFIGSVFYTFYLRQTFKQVPDELYYAAKVDGVGDFK
                     YLFKVMIPIAKPTIITITILSLMGSWNAYIWPNLVTDFQNYKLWLVSNGLSNAFRSTI
                     ELSGPNNVLNQQMAGSLIITLPLLIVFFALKKHIMRGVVRGGTKG"
     gene            complement(7198..8103)
                     /locus_tag="PLQ22_00985"
     CDS             complement(7198..8103)
                     /locus_tag="PLQ22_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH58569.1"
                     /translation="MLEWKKDGWKAAIALGPTLLIMALFTFYPIVNTFIMAFRNNYQF
                     LRGTDDGLGIQNFITVFSDPRFMTSLKNTLLMVIVSVPITVVVSILIAVALNSIERLR
                     GFFQTIFFLPYVTNTIALGLVFNTVFHKDFGLFNSIFNLGAVDWLGAETTYWRAFFVL
                     MVYTIWNGLAFKIIVLLSGLQNIDRQYYQAAAIDGASKWRIFRKITIPLLSPMILYTT
                     ITSFIGAFKVYTSVISLFGKGAGASNSFGPNNMLITVVGYIYNKLWEAGTPFGAASAG
                     SVILFVIILLITLIQRWVSKSRVHY"
     gene            complement(8105..9184)
                     /locus_tag="PLQ22_00990"
     CDS             complement(8105..9184)
                     /locus_tag="PLQ22_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58570.1"
                     /translation="MQNMGVEIRLEGLTKEFVEKKTNRITVAVDNFDVTIPAGKLVGL
                     LGPSGCGKSTTLYMISGLHKPTAGRIYFGEDDVTDLPPEQRGIGLVFQNYSLYPHMTV
                     RQNILFPLENMKVPKEEAEKRAQYMAELVQIGDLMNRKPNQLSGGQQQRVAIARALVK
                     MPRVLLLDEPLSNLDARLRMQMREEIRRIQRETGITTIFVTHDQEEAMSISDEIVVMK
                     DGVLHQKDKPQHVYNEPVDEFVVRFLGNPPINMIDGKIEDGKLIVNGRILEKVNLEDQ
                     PIHLGIRAEFFQVDPKGRLEAEIEFLEHIGRDTRVIASIEDASRTFKALVSAQTIKDL
                     EVGHKIRFNYTSYFVFNAETGERLI"
     gene            complement(9505..10098)
                     /gene="recR"
                     /locus_tag="PLQ22_00995"
     CDS             complement(9505..10098)
                     /gene="recR"
                     /locus_tag="PLQ22_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH58571.1"
                     /translation="MKYPKALEELILSFKKLPGIGPKTAERLALHILLGANEEDVKNL
                     SSNLLKALEEVHPCKNCGILTDQDFCFVCKDYTRTKQLMILENNKDMVSIEATGQYRG
                     RYHILNGVISALNGVGPDDINLGNLKQRIKDEGITEVIISTNPTLDGEMTAYYINNLL
                     KDELDVYRIGYGLPAGTNIEYANETTLIKALEGKRKM"
     gene            complement(10101..10409)
                     /locus_tag="PLQ22_01000"
     CDS             complement(10101..10409)
                     /locus_tag="PLQ22_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOH58572.1"
                     /translation="MNQQALMKKIKQLQDEMVETQRQIEATLFTASAGGVVTIEMLGN
                     KKLVKVDIDPSFEAHNSDDFEMLSDMIVAACQIAIDDIDKFTEEKMRKYQSMLGYGGF
                     "
     gene            complement(10411..13569)
                     /gene="dnaX"
                     /locus_tag="PLQ22_01005"
     CDS             complement(10411..13569)
                     /gene="dnaX"
                     /locus_tag="PLQ22_01005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOH58573.1"
                     /translation="MGYKALYRTYRPQKFSEVIGQDIIVKTLQNSIITGKISHAYLFS
                     GPRGTGKTSIARIFAKALNCHNINQETGEPCLECTPCTEIAISSSPDVIEIDAASNNG
                     VEQIRDIREKVKFLPSGAKYKIYIIDEVHMLSGSAFNALLKTLEEPPAHAIFILATTE
                     PNRVLPTILSRCQRFDFRPIAISDIYKHIKYVCEQENTNIEDEAAIALAESAEGALRD
                     ALSFLDQAIALAEETITLEIVNDITGNLSYDKLLEIAQHVEDKEIEKALKAITELYNL
                     GKETLKMVTSLISLYRDILVYKSFQHEKNEKYIFQKEQFKELAKILENNKIFYYVDIL
                     SDTQNKIKYSANPLIFLEVAIIKMINASSEDLDIIRRLNELETRLEENSGGTGGSSAS
                     VQNLERNLIRITGELSKLELPILKNKVEALERKTANTLDVEDVRSVNTKLNNYEKEIK
                     ALRNDLATLNPSYLEGRLKIIESKIDGKKFESSGPAVVSSEVDSKLLERIEEIENQIK
                     NLKSVSASVDVEEITEKVNKLLGQNQSQENEELDTLIARIEALEEAKLEEKISNYNET
                     PIVELADIEKRVRLMEEQVYRLSAENYFENEEKRKPKRLSKKVADNQIMFFGDEVITV
                     SEFENRDEQDFNFGAISKEPEEANKQEETLDEDLELEDSSEEREEDLEQEENELESEE
                     QDEDESENIDDATIEAIEVEEAEDEDVYGDAEIDKASVSYRLKYQPDAKPGKISEGYI
                     YKHEKAKEQYDITDAAYSVDKDKNKALDKEVYNLFNAKQEEKPKENKKETYATDDGIV
                     VHSTHSTIVKKQSKDDYYEQKSMGLETPKVEIEEPQEKEPAQEARVEVKSVESLADLI
                     DLVDSKPTSVEHKTSESVVEPKVVKKDFSEEEAYENYSEDIILRIMIDALDKEARDDK
                     RRITEIWPHLGESVLPQDVSVANLLKQGDVRAVGKKEIILVYESPMVCNQIMDKKTRR
                     RALSIIEDKLGDFYNYMALPESDFSEKREEFAGYYLMGSIPKKLSPVKNPYLKNTFNQ
                     KEKSITEELTDIFGPGIVKTEG"
     gene            13728..14312
                     /locus_tag="PLQ22_01010"
     CDS             13728..14312
                     /locus_tag="PLQ22_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH58574.1"
                     /translation="MKKEKKTKQIFELFWIFFKIGAFTFGGGLAMLPLIRKELVERKK
                     WISDEDMLDMLALAECTPGVIAVNSATYVGHTIGGFWGSLLATVGVILPSIIVITIVS
                     FFYQAFLENKVISQIFHGVRIGAVVLILNALSKLYRVLKKDYMSYGFIVIALALALLL
                     NIPTITIIVFGMLVGAIFAVISAKKEVETYDGIN"
     gene            14299..>14622
                     /locus_tag="PLQ22_01015"
     CDS             14299..>14622
                     /locus_tag="PLQ22_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH58575.1"
                     /translation="MALIKLLLELFIVFFKIGLFTIGGGYAMIPMIEDEIVMQKGWIT
                     TTEILNFLAISESTPGPFAINTATLIGFQQAGILGGIVATLGVVMPSFIIIYIIAKFM
                     SKFSEN"
BASE COUNT         4387 a         2751 c         2061 g         5423 t
ORIGIN      
        1 aataataact ttctcttgga tatcttttta tactttgacc taaaaacttt tcatttaaac
       61 catcagtata agtagctgct gtatcaaaat aattaacgcc gtttttatat gctttatcaa
      121 ttagtttatc accaagttca taatcaatat ctccgttagc ttttgtcttt aatctcattg
      181 ctccaaaccc aagcaaagaa gggctgatat taagtttatt catttttctg tattccataa
      241 tttgctcctt tgttctttct tatattatat cacataaaaa aaatagagcc aaataatatt
      301 tggctctatt cttaatcacc tttttaagga gctttgtgta atgttatatc gtctacaaca
      361 agtgcgtatt ctgtgtttcc atcatcataa gttgcacttg gagtattata gtaaattcta
      421 tataatcctg gtgtgaaatt agatgagatt tctacatatt catcttttaa taagtcagga
      481 aggaagtttg catcttctct gccagttaat ttaacgtcta cccattcctc gtcttgttta
      541 acttgaattt ttaaaactga atctgttaat cccataatag tttcatattt ttttgtgttc
      601 caaggagcaa ttttgaattt aatttctgct gcttcataag aagttaaatc aaattcagcc
      661 caagattctt gtccgctttt tttagttgat aaaactagca tacaaccact atttttatgt
      721 gggtcataat ttgatgagtt tgtttgtgct cttaaagtat taatgctcat tgcttcacca
      781 tcgccatttt caaattcaat agttgtttga gcataaccag ttttagctgt tgtaccgaaa
      841 tcaattttct ttacaacagt acttgctgct acactaactg taacttcata tgtgtcagtt
      901 gcttcaccaa atgtaattgt aacagtaatt tttacttctg ttgcttctgc tggagcattt
      961 acttttccat ctgctgcgat aatttccggt ttatctgtag cccatgcaac ttgtaccatt
     1021 ttatcatcaa ttggcgcttc tgcaaagaat tctaagtctt tgtctacttc cttaccagct
     1081 ggtaattcta atgctaagta ttttttagct aaagctactg tttctgcatc gtttaatgct
     1141 gtaaacacaa ctgattctgg aactaataca tatctccatt tatcatcgtc gtggtgaccc
     1201 ataactacta ataacgcaga atcaattttt ctgcctactt gtggcatatc aaattgattt
     1261 aatgtttctg gcatattatt ataataaata taaagtttgc cttctgcgcc ttctgcttcg
     1321 tcatctgcag ctgcgatata ataattatta tatctaccag caacagctaa tttaccagaa
     1381 actttataaa tcttaccaat gttagttaaa tctgtagcag gaattgcgca aagttctgca
     1441 actgtttttt caacagcttt acttgcatga tcatagcctt cgccaggagt cattacatca
     1501 actgtaatta tagcaagttg ttgtagtcct ttataagcat ctctcttacc aattacgtct
     1561 acaacatcac catatttagc tccaccatat gatgaaccat atagtaaaat tgcgccagtt
     1621 gaatcttcaa caacaatacc atctttaagt acaccagtta ctgttgcttg aacatgaact
     1681 accggcttat tagctggatc cattgccaat gcttgagcga ttgtagaaac ttcatgaact
     1741 ggtgctaaga atgttaattc aactgtttca gtaacttctc ttaaagtaat tgtagcagtt
     1801 aattttacag ttgttgttgc atccggtcta ctatatgctc cagtcttaga aagaactgct
     1861 gcattgtctg attcccaagc aatagcaatt gcttcatcta aagctgatgt tgttggtaat
     1921 ttcacgtttt tgtaaactgc atagttttct gggaatgtag tattaagatt accaatagct
     1981 gcagctactt tttcttcatc tgttaattct ggagctgcta cttctacaac agttcctgga
     2041 attacagata ctctccaacc acctgaataa gaatataaaa ctgcatctaa ctctacatat
     2101 ttatcaagtt ttgcattaaa atctgctaat ccttgaggat gtccgccata atagattgaa
     2161 attgattttc ctgaagcaag atcgtctaag tatgagttag tgtattgtcc ttctgttttt
     2221 tgaacagctt ttcctgttaa tctaacaaaa gttcctggaa gatgaatatc ttgacccatt
     2281 gcttcaactt cagctgcatt tttagcggga cctaaatcgc catatacata acccgctgct
     2341 ggagcatctt ctaatactgt aattcttgga gtatcacctg cgatttgttg acagttataa
     2401 tgattctttc tagtaccaga aaccgaaact ttagctcctg gtttaactct ttctggatga
     2461 tctgattttg ctctataaat aaagaatgaa cctgtttcat ctttgaagaa agctaaaact
     2521 ccgcttgaat cttcccatga gtgagttaca ataccttcac cttgaacatc ttgatttaat
     2581 tctgcttcga ataattctgc tactgttaaa gctcttggag ctggagcaat ataaattgtg
     2641 aactctcttg ttttagaacc atctttacct tcaatagtta aagtagcaac taaagtagct
     2701 tcaccttgct cactgccttc aggacgtgta acgcgtagca ttgtttttcc ttctgcagtt
     2761 ttttcaactt ttaaatattc tgaacctttt gagatagacc aagctacttt aactgtaatc
     2821 ctgtttccat ctgcgtcttg tacaattaca gtgtcttgta attgctcgtc ttgttttaag
     2881 ttattagcat cacctaagaa aacaattttt ccttgcgctt cttctaaata acccattgtt
     2941 ggatcttctt ctgttggcac acaagaacaa cctacaacta caaatgccat aagggcaaat
     3001 agtagtacca aacctaatct tctaagtttt ttcaaaatcg tatacctcct aattttgtat
     3061 tattttgaaa cattaacgaa atcattaatt ccgcttgttt gatttcctat cataacctga
     3121 taagtgtcat ggaattttga aaccccacct atgcctatat acttgtctcc aactacaata
     3181 ctattgtagt ctaatttagg atatgcgcca ccatcaactc taaaattgat ctctgcgcct
     3241 gtttctgttt tacagtaaat agtatacgct ccttcttcat ttggagaact aacctttgaa
     3301 acagttacat ttctcacaat aacaacatat ccttcataat cttctaaaga cgtaaagtct
     3361 gctggaactt cagtatattc tacttcggct ccatcttcat tagctaaaat tcttacttga
     3421 cttggatttg ttagttgtaa tccatagata tcgctaattc caacctgaca ttggaatcta
     3481 acaatagttc caacttttaa agcacctaaa ccagaaccat aaccactaaa gatgtagata
     3541 cctgcttttt taatttcgtc atcaaaatct gttgcctcta aatcttctaa atacaagttg
     3601 tcgcctgtaa tacgcataat ttgagcttca attaataatt ttgttccgtc gtcataatca
     3661 tcataatttt ttctaatttc tgcgattgaa acttcaacaa tctcaccagt ataattaaaa
     3721 cttggatcaa attcaccaaa aactcttctt cttgtagcgg ctgttttaga agcagcacta
     3781 atgaatgtat ctacataatt atcattatcg ccgtttgagc ccgtatagtt agaatatgcg
     3841 ttttctacaa tctctaagtt aagtaatcta aattcttcac tagcactatt gcgataccaa
     3901 actaatccta aatatctttt gccagtagca tcaagttgag cagatttgcc atcagctgtt
     3961 tctaaaacga tttgatgggc atttgttaaa atgttcccaa caaaactgct agcagctttt
     4021 ccccaaggat cgattctacc agtagattct ggtgtattaa tatacaagaa tctaagtgta
     4081 aatgttcctc tttctccctt gttacggaaa tgtgctgtat cgccatcaac taactggcct
     4141 aattctactt cgccaatacc gttagttaaa aaactttttc cttcaaaact tttttctaat
     4201 tttaaattat cggtataact agtatctaga tgaccataaa tttcaaaagt tgctgctgct
     4261 gtttttttgc cactaatagc tctaactgta actctacctg ggccaatagc agtaactaag
     4321 ccgtttttta cagtcactac tttttcatta cttgattccc acttatactt agaattctct
     4381 gggaagacag ttgcttctag ttggaaggtt tcaccaacta gaagatccgt tttctccaca
     4441 tttaacctga ttgtttcggc ttctctacaa ccaactaaaa gaaatccaaa aagtaaaaat
     4501 actaagctaa atgttattct tctaaatttg tttatcattt ttgatatgct ttacctaaag
     4561 tagcatatgc gtcttcaaga cgttttttta cttctgcttt gtttacgata actgatacta
     4621 aagcactacc tacttcatct ctaatttttg aagaacctac aaaggcaggg tctacaaaga
     4681 attgatatcc ttgagcacga tattgtaaag ctagattagc agctttagag tatgatcttt
     4741 tctctagtgg aggattgtct aaatactctt gataagcttc tgtttcataa gctgataatc
     4801 ttactggtaa gtaacctcct gatgccattg caaatttagc tgtaacttct ggttgtaata
     4861 agaatttaat aaataaccaa gcagctgctc tttctaagtt tgtagattga gctaacatag
     4921 caatattagt tccttgttgg ataacatatt ttgcatcttc tgtaaaataa ggaacagtag
     4981 atattccgat ttcaaactta ttaccttctg gttcgttata tttaatacca gctgaagatc
     5041 caactgtaaa taaaacttgt cctctcttga atgcatctga tgcgtagtta gaaccaaatg
     5101 tttctgctac tgcgaaacgt ttagctgtat tttcgtcttg gaagtatttt aagtttgcta
     5161 cgtttgctgg agtatcaaaa gctaaatatc cttgttcgtt gtttgctctt tcagtataag
     5221 tactctttac ttgtcttgta aaagtaataa atgcgttagc tgatgagtca taagcaaaag
     5281 tatagaatgt tcctctatta atatgttcag aaattttata ttcgccagct gcaacagtaa
     5341 taatgccatc taattctcct gctgccccta aagctaaaag tttatcagaa actgatttta
     5401 cttcagccca agttttagga actttaatag tggcatcttt tgccatagct gcttctagaa
     5461 tagttttgtt ataaactaaa acttctgtag atttgttaaa tggtaatgaa taataatcat
     5521 ttggatttgc atcatcaaac cctttacctt cagctaaata agaaggaata aagtcatcta
     5581 attcagcttg tgtataacca ttaggtccag caataaagtt tgataaatta atcattgcac
     5641 ctgctgaata atattctgca aagtggtctg gataacctaa tgttacagtt ggtgcttgat
     5701 atgattgaat ttccaaaatg tttgtttttc ttaattcagt atatccacca ctaattgttt
     5761 ttaatgtaat tcctacatgt ggatattctg cttcaaataa aggaattagt ggcgttaaag
     5821 aatcgctaac aacaccgctc gttacactta ctgtaatttg tactttttca atagctacgc
     5881 catctttttc tccagtctta acaacttctg ttaagtcttc gaataccttg tctcctttgt
     5941 caacttggca agaacagccc gcaactaaaa tcaagctgaa aattgcaaca agtactaata
     6001 aaaatcttct cataatttat tttcctctct ttcttatgat aaaattatta accttttgtt
     6061 ccaccacgaa caactccacg cataatatgt ttctttaaag caaagaatac aattaataat
     6121 ggtagtgtta taattaacga tccggccatt tgttggttaa gtacgttgtt tggaccagat
     6181 aactctattg ttgatctaaa cgcattagat aaaccattac ttactagcca caacttatag
     6241 ttttggaaat ctgtaactaa gtttggccaa atatatgcgt tccaagaacc cattaaactt
     6301 aaaattgtaa ttgtaataat agttggctta gcaatcggga tcataacttt aaataaatat
     6361 ttaaaatcac caactccatc tactttagcc gcataataaa gctcatctgg tacttgcttg
     6421 aaagtttgtc ttaaatagaa agtatagaag acacttccaa taaacggcaa gatcattgct
     6481 agatagtttc ctactatcct tccggtttcg gcatttaacg gagccccaac ccaacctaat
     6541 cttgatacag ttccatagtt agtaataatg aatacttcac caggcaccat cattgttgat
     6601 aataaaattg tgaataacaa gtcgcttcct ttaaatctca acctagaaaa agcaaatgcg
     6661 gaaaacacaa cagatattac tgtaaagatt gttgtaaaga aaccaactaa aattgtattc
     6721 ccaaacatat ataagaatgg tactcctcgc cttaaagttc cattatacgg atttacctct
     6781 atgcggaaaa tattcttata tgcctcaaaa gtaattttat ctggaattaa tgaaggtctt
     6841 gttgctgcga tttcttcagc tgatttaaaa gacgtaataa ccatccaata aaacggaact
     6901 aaaataaata aagcaactaa aattaaaaag atatatgtaa taacttttaa tattgttctt
     6961 tttattctaa atgcctttaa ttgatcttcc ggaacataag cttctttttc ttttcgctta
     7021 atttcttttg ctctttcaag ctcagataaa tctgcgcctg attttaatgc taattgttca
     7081 gccttaactt tttgtttatg tttacttact agataaacaa ttaagtaacc aattccaaat
     7141 aatgctgcta aacctaataa aattaataaa atttctttaa aacccattta gcgcctccta
     7201 gtaatgtacc cttgatttac ttacccatct ttgaataagt gtaatcaata gaataattac
     7261 aaatagaatt acagatcctg ctgatgctgc accaaacggt gttccagctt cccataattt
     7321 attgtaaatg taaccaacaa ctgtaattaa catgttgttt ggaccaaacg agttacttgc
     7381 accagctcct ttaccaaata gggaaattac actagtgtac actttgaacg ctccgataaa
     7441 cgaagtgatt gttgtatata aaatcattgg tgacaataaa ggaatagtta tttttctaaa
     7501 aatacgccac ttagatgcac catcaattgc ggcagcttga taatactgcc tgtcaatatt
     7561 ctgcaaacca gaaagtaaaa cgataatctt aaaggcaagt ccgttccaaa ttgtataaac
     7621 cattaaaacg aagaacgccc tccaatatgt tgtttcagct cctaaccagt caaccgcacc
     7681 taaattaaag attgagttaa ataaaccaaa gtctttatgg aaaactgtgt tgaatactaa
     7741 acctaaagca attgtgttag taacataagg taagaagaag attgtttgga aaaatcctct
     7801 taatctttca attgaattca aagcaactgc gattaaaata cttacaacaa cagttattgg
     7861 taccgaaaca ataaccatta ataaagtatt ctttagcgat gtcataaatc taggatcact
     7921 aaatacagta ataaaatttt gaattcctaa cccatcatca gttcccctta aaaattggta
     7981 gttattccta aacgccatta taaaggtgtt aactattgga tagaaagtaa acaaagccat
     8041 tataagtaaa gtaggaccta aggctattgc tgctttccaa ccatcttttt tccattcaag
     8101 catcttagat taatctttct ccagtttccg cgttaaatac aaagtaggaa gtatagttaa
     8161 acctgatctt gtgtcctacc tccaagtctt taatagtttg agcagatact aatgctttaa
     8221 atgtacgact agcgtcttca attgaagcaa ttacccttgt atctctacca atgtgttcta
     8281 agaattcgat ttctgcttct aatcttcctt ttggatctac ttggaaaaac tcggctctaa
     8341 tacctagatg aatcggttga tcctctaaat ttaccttttc taaaattcta ccatttacga
     8401 taagtttgcc atcttcaatt ttaccatcaa tcatattgat tggagggttt cctaagaacc
     8461 ttaccacaaa ttcatcaact ggttcgttat aaacatgttg cggcttgtct ttttggtgta
     8521 aaacgccatc tttcataact acgatttcgt ctgaaatact cattgcttct tcttggtcgt
     8581 gagttacaaa gattgtagta ataccagttt ctctttgaat tcttctaatc tcttcacgca
     8641 tttgcattct taaacgcgcg tctaagttag aaagtggttc atcaagaagt aatactcttg
     8701 gcattttaac taaagctctt gcaatagcaa ctctttgttg ctgacctcca gataattgat
     8761 ttggtttacg attcattaaa tcaccaattt gaacaagctc agccatatat tgcgctcttt
     8821 tttcagcttc ttcttttgga actttcatgt tctctaatgg gaataaaatg ttttgtctta
     8881 ctgtcatgtg aggatataga gagtagtttt ggaaaactaa accaattcct ctttgttctg
     8941 gcggtaaatc agtaacatcg tcttctccaa aatagattct tcccgctgtt ggtttgtgta
     9001 aacccgaaat catgtaaagt gttgttgatt ttccacaacc tgaaggtcct aataacccca
     9061 ctaacttgcc tgctggaatt gttacatcaa aattgtcaac agcaacagta attctgtttg
     9121 tctttttttc tacgaattcc ttagttagtc cctctagcct aatttcaact cccatgtttt
     9181 gcatgtcttt tttgattgct tccgcatcaa attcagacaa ttcttctcca gattctaatt
     9241 cgcttactgc ttctttagaa tctgctactg ggggtttttc ctttttctta aacagttctt
     9301 taattttgtc aaaaaatccc atatcttctc tcctattttt ttgtattaac taagtccact
     9361 taatctaaaa agtggactta gttatgttaa aatatggcac actaacgcca aaagtagtac
     9421 taactagcgt agtacccaaa gagttcatat tcataatagc ttgcttaaac aaattcatga
     9481 aatacactcc tttcatatat tatactacat tttccttttt ccttcaagtg ctttaatcaa
     9541 tgttgtttcg tttgcatatt cgatattagt acctgctgga aggccataac ctattctata
     9601 aacatctaat tcatctttca ataaattgtt aatataatag gcagtcattt ctccatctaa
     9661 tgttgggttt gtagaaataa taacctcagt aattccttca tctttaattc tttgttttaa
     9721 attacctaaa ttaatatcat ctggcccaac accatttaaa gctgaaatta caccatttaa
     9781 aatatgatat ctaccacgat attggcccgt ggcttcaata ctaaccatat ctttgttgtt
     9841 ttctaaaatc attagttgtt tggttcttgt ataatctttg catacaaaac aaaaatcttg
     9901 gtcagttaaa ataccacaat ttttacaagg atgaacttct tctaaagctt taagtaaatt
     9961 actagaaaga ttcttaacat cttcttcgtt tgctccaagt aagatatgta aagcaagtct
    10021 ttctgctgtt tttggtccta tccccggaag cttcttaaaa cttaaaatta attcttctaa
    10081 agcttttgga tatttcaaat ttaaaacccg ccatatccta gcatgctttg atattttctc
    10141 atcttctctt cagtaaactt atcgatgtca tcaattgcaa tctgacaagc agctacaatc
    10201 atgtcgctta acatctcaaa atcatcgcta ttatgagctt caaaagaagg gtcaatatca
    10261 acttttacta atttcttatt gcctaacatt tcgatagtta caaccccacc tgcagaagca
    10321 gtaaataaag ttgcttcaat ctggcgttgt gtttcaacca tttcgtcttg aagttgtttt
    10381 atttttttca ttaatgcttg ttggttcatt ttatccctcc gtcttcacaa ttcctggacc
    10441 aaatatatct gttaattctt cagtaattga tttttctttt tgattaaatg tgttttttaa
    10501 ataagggttt ttaactggag ataatttttt aggaattgat cccattaaat aatacccagc
    10561 aaattcttcc cttttctcgc taaaatcaga ttcaggtaaa gccatatagt tatagaaatc
    10621 tcctaactta tcttcaataa tactaagggc tcttcttcta gtttttttat ccataatttg
    10681 attacaaacc attgggcttt cataaactaa aattatttct tttttaccta ccgctcgaac
    10741 atctccttgt tttaataaat ttgcaacaga aacatcttga ggcaaaacac tttcacctaa
    10801 atgaggccaa atttctgtta ttctgcgttt atcatctctt gcttctttgt ctagagcatc
    10861 aatcataatt cttaagataa tatcttcaga gtaattttca tatgcctctt cttcgctaaa
    10921 atctttttta acaacctttg gttctaccac agattcggaa gttttatgct ctaccgaagt
    10981 tggttttgaa tctacaagat caattaaatc agctaaactt tctacacttt taacttctac
    11041 gcgagcttct tgagccggct ctttttcttg aggttcttct atttcaactt ttggagtttc
    11101 taaacccatc gatttttgtt cataataatc atctttagat tgtttcttta cgattgttga
    11161 atgagtacta tgaacaacaa tgccgtcgtc agttgcatat gtttcctttt tattttcttt
    11221 aggtttttct tcttgctttg cattaaataa attataaact tctttatcta atgctttgtt
    11281 cttatctttg tctacagaat aagcagcatc agttatatca tattgttctt ttgctttctc
    11341 atgtttataa atataacctt cagaaatctt tcctggttta gcatctggtt gatattttaa
    11401 acgatagcta acagaagcct tatctatttc tgcatctcca tagacatctt catcttctgc
    11461 ttcttcaact tctattgctt ctatcgttgc atcatctatg ttttctgatt catcttcgtc
    11521 ttgctcttct gattctaatt cattttcttc ttgctctaag tcttcttcgc gttcttcgct
    11581 agaatcttct aattcaagat cttcatcaag tgtttcttct tgcttatttg cttcttctgg
    11641 ttctttacta atagcgccaa aattaaaatc ttgttcgtct ctattttcaa attcagaaac
    11701 tgtaataact tcatcaccaa aaaacataat ttggttatcg gctacttttt tactcaatct
    11761 ctttggtttg cgcttttctt cattttcgaa atagttttca gcacttaaac gatatacttg
    11821 ttcttccatt aagcgaactc gcttttcaat atctgcaagt tctactattg gtgtttcatt
    11881 ataattagat atcttctctt ctagttttgc ttcttctaaa gcttcaattc ttgcaattaa
    11941 tgtatctaat tcttcatttt cttgagattg attttgccct aaaagtttgt ttactttttc
    12001 agtgatttcc tctacatcta ctgaagccga aaccgacttt aagtttttga tttgattttc
    12061 tatctcttca attctttcta aaagttttga atcaacctca ctactaacaa cagctggtcc
    12121 gcttgattca aatttcttgc catcaatttt agattcaata attttcaatc ttccttctaa
    12181 atatgatgga tttaaagtag ccaaatcatt ccttaatgct ttgatttctt tctcgtagtt
    12241 atttaattta gtgtttaccg accttacatc ttctacatct aaagtattag ctgtttttct
    12301 ttctaatgct tctactttgt tttttaaaat tggtaattct aatttactca actcgccagt
    12361 aattctaata agatttcttt ctaaattttg aaccgaagcg ctacttccgc cagttccgcc
    12421 actattttct tctaatcttg tttcaagttc atttaaacgt cgaatgatat ctaaatcttc
    12481 actagatgca ttaatcattt taataattgc aacttctaag aaaattaatg gattagcaga
    12541 atacttaatc ttgttttggg tgtcgcttaa gatatctaca taatagaaaa tcttattgtt
    12601 ttctaaaatt tttgctagtt ctttaaattg ttctttttgg aaaatgtact tttcattttt
    12661 ctcatgttga aaagatttat atactaaaat gtcgcgatat aaactaatta atgaagttac
    12721 catttttaaa gtttctttac ctaaattata aagttcagta atagctttta atgctttttc
    12781 aatttcttta tcttcaacat gttgagcaat ttctaataat ttatcataac ttaaattacc
    12841 agtaatatca ttaactattt ctaaagttat cgtttcctcg gctaaagcaa ttgcttgatc
    12901 taagaaactt aaagcatcac gaagcgctcc ttcagcactt tcggctaaag cgatagctgc
    12961 ttcgtcttca atgtttgtat tttcttgttc gcaaacatat ttaatatgtt tatagatatc
    13021 tgaaatagca attggtctaa aatcaaatct ttgacaacga gataaaatgg ttggtaatac
    13081 gcgatttggt tctgttgttg ctagaataaa aatcgcatgt gctggtggtt cttctaatgt
    13141 ttttaataat gcattaaacg cactaccact taacatatga acctcgtcaa taatatagat
    13201 tttatattta gctccacttg gtaaaaactt aaccttttct ctaatgtctc taatttgttc
    13261 tacaccatta ttacttgcag catcaatttc aataacatct ggacttgaag agatagcaat
    13321 ttctgtacaa ggtgtacact ctaaacaagg ctcgcctgtt tcttgattaa tattatggca
    13381 gtttaaagcc ttcgcaaaaa ttcttgcgat tgaagtttta ccagttcctc ttggcccact
    13441 aaataaataa gcgtgagaaa tttttcctgt aattattgag ttttgtaatg tcttaacaat
    13501 aatgtcttga ccaataactt ctgagaattt ttgaggacga tatgtcctat atagtgcttt
    13561 gtatcccata aataccaact cctcgtgcgt atattattat accataaaaa cctgcttttt
    13621 taaacagttt tcttaaagaa gttttatttt tcaaaagctg gttttcctgt ccttgttttt
    13681 atacaaattt tattgtataa ttaaaaaata gtggaggtga tgcaaaagtg aaaaaagaaa
    13741 aaaagacgaa acaaattttt gaactttttt ggattttctt taaaatcggt gcttttactt
    13801 ttggtggcgg tttagctatg ttacctctaa ttagaaaaga gcttgtagaa agaaagaaat
    13861 ggatctctga cgaagatatg ttagatatgt tggctttagc cgaatgcact cctggagtta
    13921 ttgcggttaa tagcgctacc tatgtgggcc acactattgg aggcttttgg ggttcgcttt
    13981 tagcaacagt cggagtaata ttgccatcta ttatcgttat tactattgtt tcgtttttct
    14041 atcaagcatt cttagaaaac aaagtaatta gtcaaatttt tcatggcgta aggattggtg
    14101 ctgtagtttt aattttgaat gctttaagta aattatatag agtattaaaa aaagactata
    14161 tgtcttatgg ttttatcgta attgctttgg cacttgcttt attgttaaat ataccaacaa
    14221 tcacaattat tgtttttgga atgttagttg gagctatttt tgctgtaatt agtgccaaaa
    14281 aagaagtgga gacttatgat ggcattaatt aaattacttt tagagttgtt tattgttttc
    14341 tttaaaatag gattatttac tatcggcggt ggttatgcga tgattccaat gatagaagat
    14401 gaaattgtaa tgcaaaaggg ctggattaca acaaccgaaa ttttaaactt tttagcaata
    14461 tctgagtcaa ctcctggccc atttgcaatt aataccgcaa cattaattgg cttccaacaa
    14521 gcaggcatat tagggggaat agttgctaca cttggtgttg ttatgccttc atttattatc
    14581 atatatatta ttgcaaaatt tatgtctaag ttttcagaaa at
//
LOCUS       DAOSGS010000010        14369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_3479_length_14369_cov_7.392413, whole genome shotgun sequence.
ACCESSION   DAOSGS010000010 DAOSGS010000000
VERSION     DAOSGS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_14369_cov_7.392413"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1114
                     /locus_tag="PLQ22_01020"
     CDS             131..1114
                     /locus_tag="PLQ22_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58576.1"
                     /translation="MQEAVLIGAYTNTIEKELTLVETILDWKKYNLPVILSTHYPVNS
                     NIQNLVDYYIFDKEQYLDPAIMNQHIYSCSSFEIVADFDRPYHAPAALIAYQNAIRLL
                     DNKYDFIYLQDYDVVLNKNKIFEYTRSSEFLKYKMFMCNWYNIPDSYATNIIFFRDNY
                     FTKLWGDIRIPKDFLDLVRSTGNNNLLIEGLAKRLIDVKNLKDDVFIFSNEQRDEFLG
                     EFTKHMADNNVPRIYLASTTQNQYILFIINSTGREIDFHLSGWEFITNKILNKNIRLH
                     GNLCMYWTVYNENTKLTVTYENNKKIYNILPGEIYKECNFVFKDSTPIKCK"
     gene            1131..1628
                     /locus_tag="PLQ22_01025"
     CDS             1131..1628
                     /locus_tag="PLQ22_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58577.1"
                     /translation="MVLGKAKGNKMERDTAKSLSIWMFDDEHTLKREPTSGAFKYNYC
                     GDIFPMKQINWLNFPFLIETKTGYEQHTPTLWQYNKVIEWFIKATNEGKQHNQHIILL
                     ICQFKNKPTLLFTNYQIPIERVLPVSIIPIQINGDINWTNVYLFKELLKLNFYDIFGN
                     EITYR"
     gene            1638..1979
                     /locus_tag="PLQ22_01030"
     CDS             1638..1979
                     /locus_tag="PLQ22_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58578.1"
                     /translation="MMKNLISKSFLTKDDDEQENVSEIPENDKLQDVQKCRDCDNTAC
                     KCQLIMMKIEDVIELSTQIEHTELFNIMLTIHGALKNLHDDETSLNKILSACEETYTE
                     INPKEMNKILN"
     gene            2008..>4295
                     /locus_tag="PLQ22_01035"
     CDS             2008..>4295
                     /locus_tag="PLQ22_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58579.1"
                     /translation="MNYSAIHPSSIIKQKIFVDKKTIFIVLPDTNIDISKEIYNIGFL
                     LSNCRGLVVPNKIITIGRRKFNGKAHFFDVKKKIMNPENEKIGRKLRVLNSLMIETPE
                     KEVVTSSQKSSHYYFYDTTVWAQALEYMSQHLSERVAVRQIFNELSTLYQSIKTNNAT
                     YDVEFLFLVKDQSGKMYNIFKSLRAMIKKDELKDIKFFDEYAIVADCQNVLMPLFNRE
                     DGNTNFLIQNIFKLEKFIEVNNSVTDINKEKENSIAAAPEDSSIESFNVDKTPSDSEK
                     IEKVDKEDTPFSNPKEQVRPIDPGMPEIKQKPTSLITNLVRSLQTSKLVANVDKKSDT
                     PKVEIKINQDEFRRALKTNKITDPDIVANVQIALNQYLKSSTKPTQEEAEDLVLRAIN
                     YTISGSDVVPDEYLHKPALLFNKLKQINTYKVPLTISKTDNIIEPKDIIDLKYTTGQH
                     RQRFEFETAIHENVQKLFGSLESIGTEYPIKVKKIESEVKDNNSDRLINYKVTLQNLN
                     GGRKEPYVVELNVPSPVNDKYFKLHGNSYIMSNQQFLRPVTKTDKNEVRMISNYGIVR
                     VGLANIKFNPTDLAEILKYIEIRYPKLIKEKDEEHCMFTDGSIIYLTGEKIYSSSESK
                     NVSIDIETGRLQDNKTKELLKQSKYEFLYDTILNKIHTINPADNLSKTKKALPYTWIY
                     LGALKMPLILYLWSQKGLLATLNEYGVDYEIVDKDTPEDGVFYVATKDNKFLKITPKD
                     LKEKMIVNGLSNIKLKEPIDDLT"
     assembly_gap    4296..4395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4396..6576
                     /locus_tag="PLQ22_01040"
     CDS             <4396..6576
                     /locus_tag="PLQ22_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58580.1"
                     /translation="AKIFTEPDYVINNLLTEAGILQQTEPVNPITEIMTSSRVTKGGP
                     GGVPGKRSFKKEQRNIHESQYGNMGAVSTPEYVDVGVVCHHTLSPVIINKYGSYGAKN
                     ITQLSGWQTLALDEALTPFQNQVDSDRLTLARTHQNQTTPISNAEMPLVCTGAEFIVP
                     QLASPRFVQKAKKDGIVTEVNKNKTLTVKYKDGTIDIFDIVPRLSRTKRGSYISLEMN
                     TLDEGESFKANQPIAFTKNFNKAGVYCGGKNITVAIMNYLGAGHEDSYVITKDLADET
                     LTDTVEAISIEIPQNTKIINLETEKNKHVNSGDILVEFSYENGLDEYLDMTQIDDEDP
                     ENESESGIFTSGRNSIKKLSPEGEIIDIKIFINNKNSVDKSLLQFHSELVKEQQKIIG
                     KLASVVKDKNKQLSVTDNMDLSFMNIGDHKYKGNVFLGTRIVYYIKRPKKVNLADKMS
                     NKYGAKGVISKILDESPKGEFTKKIDCFISPISILGRKNIAMIKELFLGKIFFYANEI
                     IDKMAEDPKITNDKLAKFIIDLYEITGPKKVAKQVADNINSYSGNKLRQAIKDDKINL
                     FCLVEPFEDISFEAIKSAAKFINIPLEEKVYIPELDKWTDVPVPVGISYYMFLEHYSD
                     VYANIRGSEKFVSLTRQPTKRKAQGGGQSISALDIYTFLTYDANNIMSELLGPRSDEH
                     RSKREMYNSIIETGEMPSVPEVTKSGGTRDVFNLYITGMGLNIV"
     gene            6782..7384
                     /locus_tag="PLQ22_01045"
     CDS             6782..7384
                     /locus_tag="PLQ22_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58581.1"
                     /translation="MEEKIVCTMKKFRKSSDGEKVLDVQMVECDKIKVFCQKGKAQVD
                     DTEIIDTVFVHYRDHKGRPVQLCLRENPEVTKVIREKIFKEFGKMKIKAEGNLVAFSG
                     QPCIEFVVTSDASCYERNRFKFFSYARCEPGLWKFDPIQPKRKPKIRRLDLEELGVAE
                     SVERKLGKSLGKISDTHPELAGLRIDHEKIADAASAGKRK"
     gene            7461..7898
                     /locus_tag="PLQ22_01050"
     CDS             7461..7898
                     /locus_tag="PLQ22_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58582.1"
                     /translation="MAKNITVTNIDGLDIGEKHNMEPRQKKKMIHEQLFEQYGINPNK
                     VFEKTGILKMIPNEWIPDIIIFNVKNCKTKRTYENINKNEKVISVSFESADDCQKFYD
                     EFNLSMPKSVNAFIVSQKEDPKRYNRIIVIETSTENQQINFRY"
     gene            7913..8797
                     /locus_tag="PLQ22_01055"
     CDS             7913..8797
                     /locus_tag="PLQ22_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58583.1"
                     /translation="MEATESFIEDNTNNVFENAPIEEFASSETPTTVPPLSISNYKPL
                     EENLIICQITPAKFDSFLKVLNVLVTDKASNDSLIIKESMITQNVSGAIVSSNIEDIF
                     EGKKIDLQIINPKKYLKLFKTFRNNNDIFIMDDAENSRFIISNGEIRLFLPKQIENLV
                     EAVKVPDLTNCEGLCTIKIDKATRNIITGLSSEASYVDYLIQEDVLKGIHVPDTAIYL
                     FSDYINDAKAAKLDETNADLALRSVAFLSIPAEDYEIAIGKRADGAYISVTNCNTGFI
                     KVDVFESLEIATGGNILI"
     gene            8810..9454
                     /locus_tag="PLQ22_01060"
     CDS             8810..9454
                     /locus_tag="PLQ22_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58584.1"
                     /translation="MIQSIDLNDIDSIKNVMIVPQNFVNYEELEANEEVIKDQYINFN
                     NLGVDLISNIDRTFLPDIYANMLDFVNETYLSITDFDTSTVLPSKLMETGKIIYEFVC
                     IDCYNTIVPNFLNHMNCSSLDNLDYLIQTKFKGDYSLVKANLVKIIKNITDELLNLQR
                     IDSSVQTDVMYQKLLFKYSYYMELVDFGETEMFVNNYIKPLLIKNLDSILWRTM"
     gene            9483..9707
                     /locus_tag="PLQ22_01065"
     CDS             9483..9707
                     /locus_tag="PLQ22_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58585.1"
                     /translation="MLWALFWIAIGFLIVMYIRIYIDEGGGGIAIKNSNKYKKYQEEA
                     DKRVEELLRNKPHSSKEELMEWWNDHHKKD"
     gene            9685..10134
                     /locus_tag="PLQ22_01070"
     CDS             9685..10134
                     /locus_tag="PLQ22_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58586.1"
                     /translation="MTTTKRTEKGKNMTEQDQFIKDKKYRENFEKYRNFNKQKAEIEA
                     NMDSIKEEVAKMLHEDQINEKIIELSNGEEWKGTYQTTSRTVTDLKALMEMIGPQRYG
                     QVVSNKESTFLTIRKAGKKKKDESLISTKPVDNDNSKPFIPDGTILS"
     gene            10217..10765
                     /locus_tag="PLQ22_01075"
     CDS             10217..10765
                     /locus_tag="PLQ22_01075"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOH58587.1"
                     /translation="MQIFTPKEIFFTKGVGSHKDELQSFERALRDAGIEKCNLVTVSS
                     IFPPGCKIISRSKGLKKLLPGSITFCVLSRCCSNEPKRLISASIGCAIPDDKQTYGYI
                     SEHHHFGWNEKQTGDYAEDLAASMLASTLGIEFNIDESWDKKEEIFKISGKIVRTLNS
                     TQSTIIKNKNYTTVLAAAVFVF"
     gene            10795..10953
                     /locus_tag="PLQ22_01080"
     CDS             10795..10953
                     /locus_tag="PLQ22_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58588.1"
                     /translation="MANTKCKVKKGMGGSRNGRNRCEYTEVLKKDSKKIRRRQDRQET
                     YKYENNSY"
     gene            11110..11186
                     /locus_tag="PLQ22_01085"
     tRNA            11110..11186
                     /locus_tag="PLQ22_01085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11144..11146,aa:Ile,seq:tat)
     gene            11228..11407
                     /locus_tag="PLQ22_01090"
     CDS             11228..11407
                     /locus_tag="PLQ22_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58589.1"
                     /translation="MKKKSRISKEEILLVMSQEIKEKFQCSTFKVLDWGEAMERQERN
                     LIDPQDFGKIRYALF"
     gene            11413..11490
                     /locus_tag="PLQ22_01095"
     tRNA            11413..11490
                     /locus_tag="PLQ22_01095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11448..11450,aa:Pro,seq:tgg)
     gene            11504..11590
                     /locus_tag="PLQ22_01100"
     tRNA            11504..11590
                     /locus_tag="PLQ22_01100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11538..11540,aa:Leu,seq:taa)
     gene            11712..12380
                     /gene="queC"
                     /locus_tag="PLQ22_01105"
     CDS             11712..12380
                     /gene="queC"
                     /locus_tag="PLQ22_01105"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOH58590.1"
                     /translation="MQNVVMGLSGGMDSATVLGYYYNKEYQIYPLFFNYGSKHNKYEF
                     ESAKKLAEYFETKPLKIVELDFIGKIFKSNLLENQGEIPEGHYNDSNMSLTVVPGRNS
                     IFITIMAGYAESVGADTVAVGTHMGDHYIYLDCRPIFISAMNTAIQASTGEKVHIETP
                     IQHLDKTGILKIGNSFTKPVPYELTRTCYKNQEFSCGKCGSCRERLEAFENIGRKDPI
                     QYQK"
     gene            12418..12909
                     /locus_tag="PLQ22_01110"
     CDS             12418..12909
                     /locus_tag="PLQ22_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58591.1"
                     /translation="MEITIKKSDYEAKIEDRFPVIYEHIFCLIMFSTDERNINTWKRH
                     LATPLLYLNGLDVSGTKTGKPTQERHFKLLFSDYFIDNLGDPIYKKVYNLASDVLQLK
                     RNIHSQYRTKSLMVMRTDPNFPVEKIFKKIENFMQEISRLLSTSDLARDRIYNLVDTI
                     LLE"
     gene            12916..13407
                     /locus_tag="PLQ22_01115"
     CDS             12916..13407
                     /locus_tag="PLQ22_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HOH58592.1"
                     /translation="MIKWLWALAYISSVLLANIFVDYFGIVTILGLTFPAGVIWIGLT
                     FSFRDFSQRYWGVWKIWIFIAIATFITLFMNWQVAVASVSAFLIAETIDWLIFTITKK
                     DFIHRIWISNTISTPIDSIVFVVLAFGWNWEAIYGQAIIKYISSLLVIPFIWYWSKKR
                     KLT"
     gene            13404..13589
                     /locus_tag="PLQ22_01120"
     CDS             13404..13589
                     /locus_tag="PLQ22_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58593.1"
                     /translation="MKEIYKLIADELIRHEKYCSNYGWALLMRDLGWKLADFDKEFDL
                     REFQFYYLRRDERINNA"
     gene            13582..13860
                     /locus_tag="PLQ22_01125"
     CDS             13582..13860
                     /locus_tag="PLQ22_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58594.1"
                     /translation="MREKTLIVLEGLFAGQEIEVEICKGIKHKIALIDNSLVTVALKT
                     NSDNKELSEKVYLTSDLSFNDFINMCNNMSEKDATIIAANTALIKTKK"
     assembly_gap    13899..13908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13909..14187
                     /locus_tag="PLQ22_01130"
     CDS             <13909..14187
                     /locus_tag="PLQ22_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58595.1"
                     /translation="VGKLESKKDLSGNDIPIMVDVYSLVMIPNPNNREQMKTMILPLL
                     YPFDDKAIKEVTFDKIFAVIDAPQDIQNVYIKVTTGIDVVPANTKIIQ"
BASE COUNT         5483 a         1926 c         2455 g         4395 t
ORIGIN      
        1 gggtcgacga ataacggtca aatgggagaa ctcgacaaaa gaatattttc tttttgccgg
       61 cgagagctat aatgaatgta aattcaaatt cgctagtcct aacacgatta agtgtaaatg
      121 ggagatttaa atgcaagaag cagttttaat aggtgcttat acaaatacaa ttgaaaaaga
      181 attaacttta gtagagacaa tattagactg gaagaaatat aatcttccag tcattctttc
      241 tacacactat ccagtaaata gtaatattca gaatttagtt gattattata tttttgataa
      301 agaacaatat ttagatccag caataatgaa tcaacatata tattcatgtt catcgtttga
      361 gattgttgcc gattttgata gaccatatca cgctccggcg gcgttaatag catatcagaa
      421 tgcaattaga ttattagata ataaatatga ttttatatat ttacaagatt atgatgtagt
      481 attgaataaa aataaaatat ttgaatatac aagatcatct gaatttctaa aatataaaat
      541 gtttatgtgt aattggtaca atattccaga ttcatatgct acaaatataa tattttttcg
      601 agataattat tttacaaaat tgtggggcga tatacgcatt cccaaagatt ttttagatct
      661 tgttagatct actggaaata ataatctctt gatagaaggt ttggctaaaa gattaattga
      721 tgttaaaaat ttaaaagatg atgtatttat ttttagtaat gaacaaagag atgaatttct
      781 tggagaattt acaaaacata tggcagataa caatgttcca agaatatatc tcgcaagcac
      841 aacccaaaat caatatatac tatttattat taattcaact ggtcgagaaa ttgattttca
      901 tctaagcggc tgggaattta taacaaataa gatattaaat aagaacatta gattgcatgg
      961 aaatctgtgt atgtactgga cagtatataa tgagaataca aaattaacag taacatacga
     1021 aaacaataag aagatatata atattttacc gggggaaatc tataaagaat gcaattttgt
     1081 atttaaagac tcaaccccaa ttaagtgtaa ataggagaaa atgatctgaa atggttttag
     1141 gaaaagcaaa aggaaataaa atggaaagag atacagctaa atctctttcc atctggatgt
     1201 tcgatgacga acacacatta aaaagagaac caacatctgg agcatttaag tataattatt
     1261 gtggtgatat ctttccgatg aaacagatta attggttgaa tttcccattt ctaattgaaa
     1321 caaaaacggg atatgaacag catactccta ctctttggca gtataataaa gtaattgaat
     1381 ggtttattaa agcgactaat gaaggaaaac aacataatca acatataatc ttattaatat
     1441 gtcaatttaa aaataaacca acattattat tcaccaatta tcaaataccg atagaacgtg
     1501 tcttgccagt atctattatc ccgattcaaa taaatggaga tattaattgg acgaatgtat
     1561 atctatttaa ggaattatta aaattgaatt tttatgatat tttcggaaat gaaataactt
     1621 ataggtaggt aaataccatg atgaaaaact taattagtaa atcttttcta acaaaggacg
     1681 acgatgaaca agaaaacgta tcagagattc cagaaaatga caaattgcaa gatgtacaaa
     1741 aatgtaggga ttgtgataat acagcttgta aatgtcaatt gataatgatg aaaatagaag
     1801 atgttattga attatcaact caaatagaac atacggaatt attcaatatt atgttaacaa
     1861 ttcatggtgc tttaaagaat ctccatgatg acgaaaccag tttaaataaa attttatcag
     1921 catgtgaaga aacatacaca gaaattaatc ctaaagagat gaataaaatt cttaattaat
     1981 tcaatacatg agaggttaat ttaaattatg aactacagtg cgattcaccc atcaagtata
     2041 attaaacaaa aaatctttgt tgataagaaa acaatcttta ttgttttgcc agatactaat
     2101 attgatatat ctaaagaaat atataatatt ggatttcttc tttcgaattg tagaggtttg
     2161 gttgttccga ataaaattat tacaattggt agaagaaagt ttaacggcaa agctcatttt
     2221 tttgatgtta aaaagaaaat aatgaatcct gaaaatgaga aaattggtag aaaattaaga
     2281 gtattaaata gtttaatgat tgagacacca gaaaaagaag tagttacttc atctcaaaaa
     2341 tcatctcatt attattttta tgatacaact gtttgggcac aagctctaga atatatgagt
     2401 caacatttat ctgagcgtgt tgccgttcga caaatattca atgagttatc aacattatat
     2461 caatcaatta aaacaaataa tgcaacttat gatgttgaat ttttatttct tgttaaagat
     2521 cagtccggta aaatgtacaa catttttaaa tctttacgag caatgattaa aaaggacgaa
     2581 ttaaaagata ttaaattctt tgatgaatat gcgattgtag cagattgtca aaacgtttta
     2641 atgccgttat ttaataggga agatgggaac actaattttc taattcaaaa tatttttaaa
     2701 ttagaaaaat ttattgaagt aaataattct gttactgata ttaataaaga gaaagaaaac
     2761 tcaattgcag ctgctccaga agatagttcc attgagagtt tcaatgttga taaaactcca
     2821 tctgattcgg aaaaaatcga aaaggtagat aaagaagata ccccatttag taatccaaaa
     2881 gaacaagtgc gcccaattga tcctggtatg ccagaaatta aacagaaacc tactagtctt
     2941 atcaccaatt tagttcgatc tttacaaact tctaaactag tagcaaatgt agataagaaa
     3001 tctgatactc caaaagtaga aattaaaatt aatcaagatg aatttcgaag agctcttaaa
     3061 actaataaaa ttactgatcc cgacatagtc gcaaatgtcc aaattgcatt aaatcaatat
     3121 ctcaaatctt ctactaaacc cactcaagaa gaagcagaag atttagtttt aagagcaatt
     3181 aattatacaa tttctggatc agatgttgtt ccagatgaat atttacataa gccagcgtta
     3241 ttatttaata aattaaaaca gattaacact tataaagttc cattaacaat ttcgaaaact
     3301 gataatataa ttgaacccaa agatattatt gatttaaagt atactactgg tcagcaccgg
     3361 caaagattcg aattcgagac agcaattcat gaaaatgttc aaaaactatt tggatcttta
     3421 gaatccatag gtactgaata tccaattaaa gtcaagaaaa tcgaatctga agtgaaagat
     3481 aataactcag atagacttat caattataag gtcactctcc aaaacttaaa tggtggaagg
     3541 aaagagccat atgttgtaga attaaatgtt ccttctccag ttaacgataa gtattttaaa
     3601 ttgcacggca attcatacat tatgagcaat cagcagttcc ttcgaccagt aaccaaaact
     3661 gataagaatg aagtgcgtat gatctcaaat tatggtattg tcagagttgg tttagcgaat
     3721 ataaaattta atccaacaga tcttgccgag attttaaaat acattgaaat tcgatatccg
     3781 aaactaatta aagaaaaaga tgaagaacac tgtatgttca ctgatgggag tattatctat
     3841 ctaaccggtg aaaaaatata ttcttcttca gaatctaaaa atgtctctat agatattgaa
     3901 actggtagat tgcaagataa taaaactaaa gaactcctaa aacaaagtaa atatgaattt
     3961 ctatacgata caattttaaa taaaattcat acaattaatc ctgccgataa tctttccaaa
     4021 actaaaaaag ctcttccata tacatggata tatctaggag ctttaaaaat gcctctaatt
     4081 ctttatttgt ggagtcaaaa aggattatta gcaacgctta atgaatatgg tgttgattat
     4141 gaaattgtag ataaagatac accagaagat ggagtatttt atgttgcaac aaaagataac
     4201 aaattcttaa agataacacc aaaagatcta aaagaaaaaa tgatagtaaa tggtctatcg
     4261 aatattaaat tgaaagaacc aattgatgat ttaacnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnngctaa aatctttact gaacctgatt atgttattaa taacttactc
     4441 actgaagctg gaattcttca gcagacagaa ccagttaatc cgataactga aattatgact
     4501 agtagtagag taactaaagg tggacctggc ggcgtcccgg gcaagagaag ttttaagaaa
     4561 gaacaaagaa atattcacga atctcagtat ggtaatatgg gagcggtctc aactccagag
     4621 tatgttgatg ttggggtggt atgtcatcat acgcttagtc cagtgatcat aaataaatat
     4681 ggatcatatg gagcaaagaa tattacacaa ttgtctggtt ggcagacact tgctttggat
     4741 gaagcattaa caccatttca aaaccaagtt gatagcgata gacttacatt agctcgaaca
     4801 catcagaatc agactacccc aatttctaac gctgaaatgc ctttagtgtg cacaggcgca
     4861 gaatttatag ttccacaatt agcttctcca agattcgttc agaaagctaa aaaagatgga
     4921 attgtcacag aagttaataa aaataaaaca ttaacagtta aatacaaaga tggaacgatc
     4981 gatatttttg atatcgttcc aagattaagc cgcaccaaaa gaggatcata tatatcatta
     5041 gaaatgaata cccttgacga aggtgaatca tttaaagcaa atcaaccaat tgccttcact
     5101 aaaaatttta ataaagctgg ggtatattgc ggtggaaaaa atattactgt cgcaataatg
     5161 aattatcttg gagcaggaca tgaagattcg tatgttatta ctaaggatct tgcagatgaa
     5221 accctaacag atactgttga agcaatttct atagaaattc ctcaaaatac aaaaattatt
     5281 aatctagaaa ctgaaaaaaa taaacatgta aattctggtg atattcttgt tgaattttct
     5341 tatgagaatg gattagatga gtatttggac atgacacaga tagatgatga agatccggaa
     5401 aatgaatcag aaagtggaat ttttacatct ggcagaaaca gtattaagaa attatctcca
     5461 gaaggtgaaa ttattgatat taaaatattt atcaataata aaaacagtgt agacaaatcc
     5521 cttcttcagt ttcattctga gttagttaaa gagcaacaaa agattatcgg caagctagca
     5581 tcagtggtta aagataaaaa taaacaatta tcagttaccg ataatatgga tttaagtttt
     5641 atgaatatcg gtgatcataa gtacaaaggt aatgtttttc ttggcactag aattgtttat
     5701 tatattaaga gaccaaagaa agttaatctt gctgacaaaa tgtcgaacaa gtacggtgca
     5761 aaaggtgtaa ttagcaagat attagacgaa tctccaaaag gtgaatttac aaagaaaata
     5821 gattgtttca ttagtccgat ctcaatttta ggacgcaaaa atatagccat gattaaagag
     5881 ctttttttag gaaagatatt tttctacgca aatgaaataa tcgataaaat ggcagaagat
     5941 ccaaaaatta caaatgataa attagcgaaa tttatcattg acttatatga aattactggt
     6001 ccaaaaaagg tagctaaaca agttgctgat aatattaatt cttactctgg aaataaacta
     6061 agacaagcta ttaaagatga taaaattaat ttattttgtt tagttgagcc ttttgaggat
     6121 atcagttttg aagcgattaa gtcagctgct aaatttatta atattccatt agaagaaaaa
     6181 gtttatattc cagaacttga taaatggaca gatgtacctg ttcctgttgg gatttcatat
     6241 tatatgttct tagaacatta ttcagatgtg tatgccaaca ttagaggatc tgaaaagttt
     6301 gttagtttaa ctcgccaacc aacgaaaaga aaagctcagg gcggtggaca aagtatttct
     6361 gccctcgaca tttatacatt cctcacatac gatgctaata acattatgtc agaacttctt
     6421 ggtcctcgta gtgatgaaca tcgttcaaaa cgtgaaatgt ataatagtat tattgaaacc
     6481 ggcgagatgc catctgttcc agaagttaca aaaagtggtg gaactagaga tgtctttaat
     6541 ctttatataa ctggaatggg attaaatatt gtgtaaaaaa tattttctat tattgtcaat
     6601 ttttatatag aaatattata tttgataatc aagagagata aattattaga ttatctctct
     6661 tgattaattt tttaaaattc aagattttta tatagaaata atataattag aaaaagttct
     6721 catagtgagg actttattta atttaacagt aacaccaaaa aattcgagga ggcatcaaat
     6781 tatggaagaa aagattgtgt gcacgatgaa gaagttccgg aaaagtagcg atggagagaa
     6841 agtactggat gtgcaaatgg tcgaatgtga caaaatcaag gtattttgtc aaaagggcaa
     6901 agcacaggtg gacgatactg aaattattga cactgtattc gtgcactatc gtgatcacaa
     6961 aggtcgccca gtgcaattat gtcttcgtga aaaccccgaa gtaacaaaag ttattcggga
     7021 gaaaattttc aaggaattcg gcaagatgaa aattaaagcc gaagggaacc tggttgcttt
     7081 ttcgggacaa ccatgtattg aatttgttgt aacatcagac gcctcctgct atgaacgaaa
     7141 tcgtttcaaa tttttcagct acgccaggtg tgagcctggt ttgtggaaat ttgatccgat
     7201 tcaaccgaag cgcaaaccta agatccgtag actggatctt gaggaattgg gtgtggctga
     7261 atctgtcgag cgaaagcttg gcaagagtct gggaaaaatt tctgatactc acccagagtt
     7321 agcgggtctc agaatcgatc atgaaaaaat tgccgatgca gctagtgctg gcaaacgcaa
     7381 gtaagaacaa agacagaggg gaatatatca ttattcccct ctgtctagct atttattttg
     7441 ttttaatgag gtatggaaat atggcaaaaa atataacagt aacaaatatc gatgggttag
     7501 atattggaga aaaacacaat atggaacctc gtcaaaaaaa gaaaatgatt cacgaacaat
     7561 tatttgaaca atatggaatc aatccaaaca aagtattcga aaaaactgga atattaaaaa
     7621 tgattccaaa tgaatggata ccagatatta ttattttcaa tgttaaaaac tgtaagacta
     7681 aaagaacata cgaaaatatc aataaaaatg agaaggttat tagcgttagt ttcgagagtg
     7741 ccgatgattg tcaaaaattc tatgatgaat tcaatctaag tatgccgaag tcagtaaacg
     7801 cttttatagt ttctcaaaaa gaagatccaa aaagatataa cagaattatt gttattgaaa
     7861 cttcaacaga aaaccaacaa attaatttta gatactaaaa atcggaggca caatggaagc
     7921 aactgaaagt tttattgaag ataatactaa taatgttttt gaaaatgctc ctattgaaga
     7981 atttgcatct tcagaaactc caacaacagt accaccttta tcaatttcaa attataaacc
     8041 attggaagag aatttgatta tatgtcaaat tactcctgca aagtttgata gttttttaaa
     8101 ggttcttaat gtactagtta cagataaagc tagtaatgat tcgttaatta ttaaggaatc
     8161 tatgataact cagaatgttt ctggagctat cgttagttcc aatattgaag atatttttga
     8221 aggaaagaag atagatttac aaattattaa tcctaagaag tatttaaaat tatttaaaac
     8281 atttagaaat aataatgata tttttattat ggatgatgca gaaaattcta gatttataat
     8341 ttctaacggc gagatcaggt tatttctgcc aaaacagatc gaaaatttgg tagaagctgt
     8401 gaaagttcct gatctcacaa attgtgaagg attatgtact atcaaaattg ataaagctac
     8461 tagaaatatt attaccggac tttcaagcga agcgtcatat gttgattatc ttatccagga
     8521 agatgtacta aaaggtattc atgttcctga tacagcaatt tatttatttt ctgattatat
     8581 taatgacgca aaagcagcga aattagatga aacgaatgca gatttagctt tgcggagtgt
     8641 agcattttta tctatacccg cagaggatta tgaaatagct attggcaaac gagctgatgg
     8701 tgcatacatt tcagtaacaa attgtaacac tggatttatt aaggttgatg tatttgagtc
     8761 actggagatt gctactggag gaaacattct aatttaagga gttcaaaaca tgattcagtc
     8821 tatagattta aatgacattg attctattaa aaatgttatg atcgttcctc aaaatttcgt
     8881 taattacgaa gagctagaag ctaatgagga agtcataaaa gatcagtata ttaatttcaa
     8941 taatcttgga gttgatttaa tcagtaatat cgatagaact tttttacctg acatatatgc
     9001 aaatatgtta gattttgtca atgagacata tttgtcgatt acggattttg acacttcaac
     9061 tgtattacct agtaaattga tggaaacagg aaaaattata tatgaatttg tatgcattga
     9121 ttgctataac acaattgtac caaatttttt gaaccacatg aattgtagtt ctctagataa
     9181 cttagactat ttaattcaaa ctaaatttaa aggtgattat tcattagtta aagcaaatct
     9241 agtcaagata atcaagaaca tcacagatga gctactaaac ctacaacgga ttgattcttc
     9301 tgttcaaaca gatgttatgt atcagaaact tctgtttaaa tactcctact atatggagct
     9361 cgttgatttt ggcgaaacgg aaatgtttgt taacaattat ataaaacctt tactaatcaa
     9421 gaacttagat tcaattctat ggagaacgat gtagctcttc cacagaaagg gatcataata
     9481 gtatgttatg ggcgttattt tggattgcaa ttggcttcct aatcgtcatg tatattcgca
     9541 tatatattga tgagggtggt ggagggattg caataaaaaa ctcaaacaaa tataaaaaat
     9601 atcaagaaga agcagacaag agagtagaag agttactaag aaataaacca catagttcaa
     9661 aagaagaact tatggaatgg tggaatgacc accacaaaaa ggactgaaaa aggtaaaaat
     9721 atgacagagc aagatcagtt tataaaagat aagaagtatc gagaaaattt tgaaaagtat
     9781 cgaaatttca acaaacaaaa agctgaaatt gaagctaaca tggacagtat caaagaggaa
     9841 gttgctaaaa tgttacatga agatcagatc aatgaaaaaa tcattgaact ttccaatggt
     9901 gaagaatgga aaggaacata ccaaacaaca tctagaactg taacagatct gaaagctcta
     9961 atggagatga ttggccctca aagatatggt caagttgtta gtaataaaga atcaacattt
    10021 ttaactatta gaaaagctgg taagaagaaa aaagacgaga gtttaatttc tactaaacca
    10081 gttgataatg ataattcaaa accatttatt ccagatggaa caattttgag ttaaatttat
    10141 tggagtaaag ctcttaaatg agctttactc caaattttat ttattaattt ttcttaaaag
    10201 gaggctagaa aatctgatgc aaatttttac tcctaaagaa atctttttca ccaaaggagt
    10261 tggatctcac aaagatgaac ttcaatcgtt cgaaagagcg ttgagagatg ctggaattga
    10321 aaaatgtaat ttagttactg tatcaagtat ttttccacct ggttgtaaaa ttatttcaag
    10381 atcaaaaggt cttaaaaaat tgttacccgg ttcaataact ttttgtgtat taagtagatg
    10441 ttgtagtaac gaaccaaaaa gattaatatc agcatcaatt gggtgtgcaa ttccggatga
    10501 taaacaaaca tatggataca tctctgaaca ccaccatttt ggttggaatg aaaaacaaac
    10561 aggagattac gctgaagatc ttgccgcatc gatgctggct tctacattag gaattgaatt
    10621 taatattgat gaaagttggg ataaaaaaga agaaatattt aagatcagtg gtaagatagt
    10681 tagaacctta aattccactc aatcgacaat tattaaaaat aaaaattata caacagtttt
    10741 agcggcagca gtctttgtat tctaaaaaaa tatatattac atgtgggaga gaatatggct
    10801 aatacaaaat gtaaagttaa aaaaggtatg ggcggatcac gtaatggtag aaatcgttgt
    10861 gaatatacag aagttttaaa aaaagattca aaaaagattc gcaggcggca agatagacaa
    10921 gaaacttata aatatgaaaa taacagctat taattctatc ggcaagatct gaaattaggt
    10981 cttaccattt ttttgatttt tttgattttt atatagaaat aatatgaata cattcaggga
    11041 tcatctaaag gtaggatgga agaatttgag tcttctcatc taggttcgag tcctagtccc
    11101 tgagccaata ggaccatagc tcaattggtc agagcagtcg ccttataagc gacaggttgt
    11161 tggttcaagt ccaactggtc ctaccatttt ttaataaaaa cttatacaaa aaagaaagga
    11221 gaaagaaatg aagaaaaaaa gtagaatatc aaaagaagaa attttattag taatgtcaca
    11281 ggaaattaaa gaaaaattcc aatgtagcac atttaaggta ctggattggg gtgaagctat
    11341 ggaacgtcaa gaaagaaatc ttattgatcc acaagacttt gggaagataa gatacgcatt
    11401 attttaatct ttcggggtgt agctcagtct ggtcagagtg ctagttttgg gaactagaag
    11461 ccgagcgttc aaatcgctcc accccgacca gtttaaagaa cgtgcctcgt tggtggaatt
    11521 ggtagacaca aaggacttaa aatccttcga tcgcaagatc atcccagttc aactctggga
    11581 cgaggcacca tatacaaacc aatcgaaagc tggaactaac acttccagct ttcgacattt
    11641 tttaattatt acgaatatta tatagaaatt atatttatga aaattaatat ttcttttttg
    11701 gaggcaaaaa aatgcaaaat gtagtaatgg gtctttctgg tggaatggac agtgcaacag
    11761 tgctcggata ttattacaat aaggagtatc aaatttatcc attatttttt aattatggat
    11821 caaaacataa caagtatgaa tttgaaagtg ctaaaaaatt agcagaatat tttgaaacaa
    11881 aaccgctcaa aattgttgaa ttagatttta ttggtaagat ctttaaatca aatcttcttg
    11941 aaaatcaagg agaaattcca gaaggacatt ataatgacag taacatgtct ctgacagtcg
    12001 ttcctggtag aaacagtatt tttattacta ttatggcagg atatgcagaa tcagttggag
    12061 ctgatactgt cgctgttgga acacatatgg gagatcatta tatatatctt gattgtcgtc
    12121 cgatattcat cagtgctatg aatacagcaa ttcaagcaag tactggtgaa aaagtacata
    12181 ttgaaacccc aattcaacat ttagataaaa ctggtattct taaaattggg aatagtttca
    12241 ctaaaccagt tccatatgaa cttacaagaa catgctataa aaatcaagaa ttttcttgtg
    12301 gtaaatgtgg cagttgtaga gaacgtttag aagcttttga aaatatcggg agaaaagatc
    12361 caattcagta ccaaaagtaa aaaaaggatt ttgaatatgt tgagatcatt tgtacttatg
    12421 gaaatcacta ttaagaaaag tgattatgaa gctaaaattg aagatcgttt cccagtaatt
    12481 tatgaacata tattttgttt aataatgttc tctactgatg aacgaaatat aaatacttgg
    12541 aaacgtcatc tagcaactcc attattatat ttgaatggat tagatgtatc tggaactaaa
    12601 actggaaaac caactcaaga acgacatttt aaattattat tttcagatta ttttattgat
    12661 aatcttggtg atccaattta taaaaaagta tataatttgg caagtgatgt cttacaactg
    12721 aagcgtaata tacattctca atatcgaaca aaatcattaa tggtaatgag aactgatccg
    12781 aattttcctg tggagaaaat tttcaagaaa atcgaaaatt ttatgcaaga aatatcaaga
    12841 ttattatcaa ctagtgatct tgcaagagat agaatttata atttagttga cactatatta
    12901 ttggagtgag ttaatatgat caaatggctt tgggcgttag cttatatttc aagtgttcta
    12961 ttagccaata tatttgttga ctactttgga attgtaacaa ttttgggatt aacttttcca
    13021 gccggcgtga tctggattgg tcttacattc tcatttcgtg atttttctca acgatactgg
    13081 ggtgtatgga aaatatggat atttatagct atcgctactt tcattacatt atttatgaat
    13141 tggcaagtag cggtagcttc agtatcagcg tttctaatag cagaaactat agattggcta
    13201 atattcacta ttactaaaaa agatttcatt catagaattt ggatttcaaa tactatttca
    13261 acaccaattg attcgattgt gtttgttgta ttagcgtttg gttggaactg ggaagcaatt
    13321 tatggtcagg ctataataaa atatataagc agtttattgg ttattccatt tatttggtat
    13381 tggtctaaaa aaaggaaact gacatgaaag aaatatataa acttatcgca gatgagctta
    13441 tccgacatga aaaatattgt tccaattacg ggtgggcact acttatgaga gaccttggtt
    13501 ggaagttagc agattttgat aaagaatttg atcttagaga atttcaattt tattatctta
    13561 gaagagacga gaggatcaat aatgcgtgaa aaaacattaa tagttctaga aggactattt
    13621 gcaggacaag aaattgaagt tgaaatatgc aaaggaatca aacataaaat tgcattaatt
    13681 gataactctt tagtaacagt tgccctaaaa actaattcag ataataaaga actatccgaa
    13741 aaagtttatt taacaagtga tctatctttc aatgatttta taaatatgtg taataatatg
    13801 tcagaaaaag atgctacgat tatagctgct aatactgctt taatcaaaac taaaaaataa
    13861 taagaaatgg agtaagaaat tatgcaaatt gttattggnn nnnnnnnngt gggtaaatta
    13921 gaatcaaaga aagatctgtc cggtaatgat attcctatca tggtcgatgt ttattctctg
    13981 gtaatgattc caaatcccaa caatcgagaa cagatgaaaa caatgattct accactgtta
    14041 tatccattcg atgataaagc tatcaaggaa gtgacattcg acaaaatatt cgcagtaata
    14101 gatgctccac aagatattca aaacgtatat attaaagtaa caacagggat tgatgttgta
    14161 ccggcaaata ccaaaattat tcagtgaagg aaaacaagca tgatcaagat ccaagtaata
    14221 ggatcagcta atagtggtaa atcaacgctt gcaaaaataa taagtagatc tttagataat
    14281 ttaaatatta atacaaaagt aattgatgaa gaacaaaatc aaaattatga taataatgat
    14341 ctattaaaac ttatcaaaat catagaaaa
//
LOCUS       DAOSGS010000011        13983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_3620_length_13983_cov_5.280586, whole genome shotgun sequence.
ACCESSION   DAOSGS010000011 DAOSGS010000000
VERSION     DAOSGS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_13983_cov_5.280586"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLQ22_01135"
     CDS             complement(<1..1154)
                     /locus_tag="PLQ22_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH58596.1"
                     /translation="MIFGRHINKYYIKYLWLFIIGILSLLAVDYLQLKIPEFLGIITD
                     GLARDPDTGIIAMGKEDLAQKILEILGIVGLIFITRFLWRVTLSANGPRIEADLRSKM
                     FAHSMTLSQDYYSKHKTGDIMAIYTNDLDIIKQSISMGTLMFFDTIFLGVLTAVKMFM
                     LNYQMALFVVLPLIVMGGFGILIGRVMQKRAAARQAAFADLSDFTVENITGLGVIKAF
                     VKEQNEIIHFAKKNKDNYDKNMSFAKASASIRIIINLFINFILIGVFAYGAYLVFDQK
                     TLTTGQLITFISLFGHLIWPIMGMSMFINMHAQAKASLQRVNGLLDYKPDIVDREDVV
                     EVEKLSGEIEVTNLSFKYPGLEENVIEDVSFKIKAGESVGILGKTGSGKTT"
     gene            complement(1288..1770)
                     /locus_tag="PLQ22_01140"
     CDS             complement(1288..1770)
                     /locus_tag="PLQ22_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH58597.1"
                     /translation="MYKSTLAFLVIDDKVIMINRNKKPWMGCWNGVGGKLEKGEDKTV
                     SVIREIEEETKIKKEDYDLEYKGILTWSVDNNQTIYGLYIFVCKLNKLNLKFPIKTSE
                     GILDLKEINWVIDHNNLGVSHNLKYILPYALYKKELYRYHCLFDGNTLLSVDITLLEE
                     "
     gene            complement(1818..5372)
                     /gene="nifJ"
                     /locus_tag="PLQ22_01145"
     CDS             complement(1818..5372)
                     /gene="nifJ"
                     /locus_tag="PLQ22_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH58598.1"
                     /translation="MSEKKKVLQAMDGNEAAAYTSYAFTEVAGIYPITPSSPMPEHVD
                     DWANKGKKNLFGNVVKVVEMQSEAGAAAAVHGSLQTGALTTTYTASQGLLLKIPNMYK
                     ISGELLPGVIHVAARAIAAQALSIFGDHQDVMAARQTGFAMLATGSVQEVMDLGGVAH
                     LASIKGSIPFVHFFDGFRTSHEIQTVEVMDYEFYKRQIDMDAVKKFRENALNPHKPVT
                     RGSAQNDDVYFQTREVSNKYYNALPDIVNHYLEEVSKETKRKYAPFVYYGHPEATDVI
                     VAMGSVTQTVKEVVDYLTSKGKKVGLLTVYLYRPFSEEYFFKAMPKSVKRIAVLDRTK
                     EPGAHDEPLTLDVKAVYYGKKNAPMIIGGRYGLSSKDTTPGQIFAVYDNLKLGEKAKR
                     EFTIGIVDDVTHLSLPVTKEIRVGSPNATNLIFYGIGGDGTVGANKNTIKLIGENTDF
                     YAQAYFAYDSKKSGGVTRSHLRFSPDPIQSPYLIPEADFISCSLDTYVQKFDILDNLK
                     KGGTFLLNTTRSKEELEDFLPNKFKRALAEKKIKFYIIDAIHAAQKIGLGHRTNTILQ
                     SAFFKLSPQIMDYEKAKGLMKQAAHDSYISKGQAVVDMNHKAIDLGAKGLKKVAVPAA
                     WKDLKDQKKKLVKKPDFVARIADEINALRGEKLPVSAFEKYADGTMAPGTAAYEKRYV
                     AAQVAHWLPENCIQCNQCAFVCPHAVIRPFLATEEELKNAPEGVVTLPAIGRGLEGLQ
                     YAISISIADCTGCGVCIEQCPGRRGEKALTFKEIEGEIENKMQEKAEFFLHEVSNKAH
                     LMDVKANAKNSQFAVPLFEYSGACAGCGETPYVKLATQLFGDRMVIANATGCSSIYGG
                     SYPTTPYTCNKEGFGPAWANSLFEDNAEFGYGMVIAHGVIRDKIAKEFNALIEAGKND
                     KLVELAKAWLENKDDGDKTKELYPELMKELEKDKSKEAKAVLGMHEHLIKRSQWIIGG
                     DGWAYDIGFGGLDHVIANHEDVNILVLDTEVYSNTGGQSSKASQTGSIAKFTAGGKTG
                     KKKDLAAIAMSYGHVYVASIAHGANQAQMVRAFREAESYKGPSIIIAYSPCIAHGIRG
                     GMSKSQVQAKLAVEAGYWTLFRYDPRLEAEGQNPFKIDSKEPEWDKYEAHLLTENRYR
                     QLRGSNPEQADFLLKLNLAESQRRYRTYQRFEAMDYSK"
     gene            complement(5485..7281)
                     /locus_tag="PLQ22_01150"
     CDS             complement(5485..7281)
                     /locus_tag="PLQ22_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58599.1"
                     /translation="MKRLRRMRRNIKYFFPILLKVNPFSIVFILLSALIKAGFGVATV
                     YLPKLIIENLMLEPKPDFMAVFIIAISYCLLLGFTYVSTKILDMINTYYAFKADEKIE
                     AMFNEKVVTIDYFNIEDPKFADDIAFARQSLWAYSGGIYSFVRTVRSIIESILTMISI
                     IGVIIFSGLPWYITLIFIVIVILYATFGYIIQTKREDIYEEHNKSIVRTSRKQWYYNS
                     SVLNFRMHKLLRSYNSEPLLDKYSDGINKEVNDKNIELTKKYSKASLGYFTNYYFVGV
                     LLVVLVLVFSYFKFGITIATLTALYTAVRNLSNQLYDAVFTMVSYDRDCIYQENYINF
                     MEKESIFKNGTIKLESIKTIEFKNVSFKYPRTEDYVLQDLSFKINDKERVSLVGVNGS
                     GKTTIIKLMCRFYDVDDGEILINGINIKDYDYDSYMKQLAVVFQDFKVISFTIKDNIM
                     IDEENHEKLYDCLERAQVLDKVLSFPNKENTYINRWFDPNGVEFSGGELQKFAFARTL
                     YKDASFVVLDEPTSALDVISEDKIYSDFNEIVGEKTCLYISHRLSSCLFCDKIIVLEG
                     SKIIETGTHYELMKLDGHYAKMFKAQAKYYND"
     gene            complement(7278..9104)
                     /locus_tag="PLQ22_01155"
     CDS             complement(7278..9104)
                     /locus_tag="PLQ22_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58600.1"
                     /translation="MKKAKKEKVKKLKNKGIFSNIIFSMKQINKVDKWYVPLLFVLEI
                     LRIPQVFLFPYILTVIVDGIERGKDARDVILKAMLVTLICFIVRVLQMLVESNIDTYR
                     KEKVIVSLQKKFYRNSLKIDYEKFESSETQDAFEKAKRALYQNGGMLTIVRSTAEFIG
                     KIFSLAIAATIIIYINLWLGVIIFGLAIIKFFLNKYARKKEQTEYRDKLPNINRRISY
                     TDNISRNLSIGKDLRIYKMDKFINKERDRTYTNFFGILKPYLIRETTVGSFINILEAI
                     DLLAIYGFLIYAVLTKGMLIATFVYMLAAVREFAWSLDVMVLSSGDFMSCSYIIKDYR
                     EFYEMNVFEKKETSDHPSNKVEIEFKNVFYSYENQEGYALNDVSFKIKAGEKIALVGL
                     NGAGKTTLVKLLCGFYKPTKGEILINGVNINQFSRKTFQELIAPVFQDNVTIGMELGE
                     NISMSPREESDEEKIKQTLKIMDLTSKVDSLPEKEKAILSREFTDTGIELSGGEVQKL
                     AIARAIYKAAPLVILDEPTAALDAFAEYNLYNNLSEFIGNSTAIYISHRLAVTKICDR
                     VLLLDDGVLIESGSHNELMKKDTEYKKLFNIQAKYYKEGGAL"
     gene            complement(9386..10210)
                     /gene="rsmI"
                     /locus_tag="PLQ22_01160"
     CDS             complement(9386..10210)
                     /gene="rsmI"
                     /locus_tag="PLQ22_01160"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOH58601.1"
                     /translation="MKGKLYIVPTPIGNIKDITLRALDILNEVDLVYAEDTRVTKILF
                     SHYNIKTQLYSYHMFNEADVSEQIITKLNQGLNIAMVSDAGMPGISDPGYLIISKAIK
                     EDIEVTVLPGPTAYITAVVGSGMPINRTLFYGFLEHKKSAKLKELEALVDNKETLIFY
                     ESPKRLDETIKLMAEVFGNRKVVIARELTKKYEEYIRSTLDELSKQDLNLKGEITIIL
                     EGAKIDSLALKLNELDILNHYNYYIDLGFSDKDAMKEVAKDRGISKSVVYKKILEK"
     gene            complement(10211..10948)
                     /locus_tag="PLQ22_01165"
     CDS             complement(10211..10948)
                     /locus_tag="PLQ22_01165"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOH58602.1"
                     /translation="MTKLYLHELLGYENIKIYQKDKNFKFSLDSMLLADFIKPKEKDI
                     IVDLGTGNGPIPLFLTLKTKSKIYGVEIQEEICELFVKSIEINGFEKQIIPVNKNLKG
                     VYKTIGANKFNIVCSNPPYFKYKEGSNVNKSRELTIARHEVLVCLEDIVKEATKLLVD
                     GGSFYLVHRTFRLSEVLLLLKENNFGVKRLKFIYPKIESDALIFLLEAKKNRKDDVVV
                     ERPLVVHNEDGKYTDEVLKIFNFKKEE"
     gene            complement(10941..11792)
                     /gene="ricT"
                     /locus_tag="PLQ22_01170"
     CDS             complement(10941..11792)
                     /gene="ricT"
                     /locus_tag="PLQ22_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOH58603.1"
                     /translation="MVKVVGIAFKPVGKIYWFNPHYLSINEGDYVVVETVRGVEIGLV
                     VQGVMEVDNNKVKDLKSVLRLATKKDVEQYKKNIARKPEALEICQELIDKNKLPMKLL
                     DAEYTLDNKKIIIYYTAEDRVDFRELLKDLASAFRLRIELRQVAIREGAKFIGGLGVC
                     GRETCCTSHIREFSPVTMKMAKDQGMNLSANKVAGVCGKLMCCIGFESEFYQEQRKKI
                     PGVGDIVKTPTCESCMVVDVDYLRELVTVKKDEDKLETFNASLVKSLKRKVEEVREED
                     LTDDIDD"
     gene            complement(11785..12774)
                     /locus_tag="PLQ22_01175"
     CDS             complement(11785..12774)
                     /locus_tag="PLQ22_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58604.1"
                     /translation="MNFSEFLENQPQVAKLLNKSVDTNHLVNVYLFDGPSGTHKLEAA
                     YYLSALIFCQAQEKPCFNCAHCKRILDRTHPSVFHLKAEKNMITKPQVDALIREFSLT
                     ALEEGKRVFIIEGIEKANKSASNSLLKFLEELSATDYGVLITENLSSVIETIKSRGQI
                     ITFKKAREKDVIALLTEEKVPENIAKIVVKLTNSLEKGLDIANDPDFLETYDLAKKII
                     KNLLLGNESPIITINLLGKKFFKLEDRKYHQYFLDMLVYFYNDILMYQLGNIEDIVYF
                     DLLNNIDGEIEMEYEKIIANIELLLEAKNKLNFNINIDLMFFDLFLEMEKING"
     gene            complement(12775..13407)
                     /gene="tmk"
                     /locus_tag="PLQ22_01180"
     CDS             complement(12775..13407)
                     /gene="tmk"
                     /locus_tag="PLQ22_01180"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOH58605.1"
                     /translation="MFITFEGGEGTGKTSIINLIYQDLIKLGYKVYKTREPGGSKIAE
                     QIRDVILDVKNTELDYKAEALLYAASRRQHLMEVVKPKLDDGYVVLCDRYVDSSLAYQ
                     GYARGLGIREVLEINRYAIDDYFPNLTIFIDLDPKVGIERIQSNRSSDLNRLDLERMA
                     FHNKVYEGYHELVKMYPERIKVVNGHQTLEEVYKSCYKLVLETLNKGKDK"
     gene            complement(13499..>13983)
                     /locus_tag="PLQ22_01185"
     CDS             complement(13499..>13983)
                     /locus_tag="PLQ22_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOH58606.1"
                     /translation="PQDIYVNNVEKVDDNFHARFNAKAKTYRYLLDLNEVNPLLVNYR
                     YYYKGPKLDLSKLDEIPKIFIGTKDFKSFSKGNEKENTVRTIYTFTIKNIDNLLVFEI
                     KGDGFLHNMIRLIIGMILEVLKGKLGVNKLKEILDSKDQKLTPKLVPGSGLYLVSVEY
                     "
BASE COUNT         4737 a         2599 c         1539 g         5108 t
ORIGIN      
        1 gtagttttac cagatccagt tttacctaag attccaacac tttcgccagc cttaatttta
       61 aaactaacat cttcaataac attttcttct aaaccaggat atttaaacga taaattagta
      121 acttcaattt ctcccgataa tttctctact tcaacaacat cttctctatc tacaatgtca
      181 ggtttataat ctaataaacc atttactctt tgcaaacttg ctttagcttg agcatgcata
      241 ttaataaaca ttgacatacc cataatcggc caaattaaat gtccaaataa agaaataaaa
      301 gtaattaatt gtccagtagt taaagtcttt tgatcaaata ctaaatatgc cccataagca
      361 aaaacaccaa taagaataaa gttaataaat aaattaataa taattctaat agaagctgat
      421 gctttagcaa aactcatatt tttatcataa ttgtccttat tcttttttgc aaaatgaata
      481 atttcatttt gttcctttac aaaagcttta ataactccta aaccagtaat attttcaact
      541 gtaaaatcag ataaatcagc aaaagctgct tgtctagctg ctgctctttt ttgcatgact
      601 ctcccaatta aaattccaaa tccacccatt acaattaaag gtagaactac aaataaagcc
      661 atttgataat taagcataaa catcttaaca gcagttaaaa ctcctaaaaa gatagtatca
      721 aaaaacatta aggttcccat gctgattgac tgtttgataa tatctaagtc attcgtataa
      781 attgccataa tatcaccagt cttatgttta ctataatagt cttgagataa agtcatacta
      841 tgagcaaaca tcttacttct caaatcagct tcaattctag gaccattagc gcttaaagtt
      901 actcgccata agaatctagt aataaagatt aacccaacta tccccaaaat ctctaaaatc
      961 ttttgcgcta ggtcttcttt acccatagcg ataattcccg tgtctggatc tctagctaaa
     1021 ccatcagtaa tgattcctaa aaattctggt attttaagct gcaagtaatc aacagcaagt
     1081 aatgacaata ttccgataat gaatagccat aaatatttta tataatactt gttgatatgt
     1141 cttccaaata tcataaattt tcctccgaat attaaataag tatatcacgt attgtctact
     1201 tttgcaatac aaacgaaaac tattttttcc ttctaatatg ttttcatttc tattaaaaat
     1261 atctgctact tagagtagca gatgttttta ttcttctaac aatgttatat ctacacttaa
     1321 tagtgtatta ccatcaaata aacaatgata gcgataaagc tcttttttat ataaagcata
     1381 tggcaaaata tatttcaagt tatgactgac tcctaaattg ttatgatcaa taacccaatt
     1441 tatctctttt aaatctaaaa tgccttcgct agttttaatc ggaaacttta aatttaattt
     1501 atttaactta caaacaaata tataaagtcc ataaattgtt tgattattat caacagacca
     1561 agttaagatt cctttgtatt ctagatcata gtcttctttc ttgatttttg tttcctcttc
     1621 aatttcccta ataacactaa ctgttttatc ttcgcctttt tctaacttac caccaacacc
     1681 attccaacac cccatccaag gttttttgtt tctgttaatc ataatcactt tatcatcaat
     1741 tactaaaaag gctaatgttg atttatacat aatatctcct tgaaaataaa gggaagatta
     1801 ctcttccctt tttaatttta ttttgaataa tccattgctt caaatctttg atatgtacga
     1861 tatcttcttt gagactcagc taagttaagc ttcaataaga aatcagcttg ttctggatta
     1921 cttccacgta attgacgata tctattttca gtcaataagt gagcttcata tttatcccat
     1981 tcaggttctt tagagtcaat cttgaatgga ttttgtcctt cagcttctaa tcttggatca
     2041 tatctaaata atgtccaata accagcttct acagcaagtt tagcttgaac ttgtgactta
     2101 gacataccac ctctaatacc atgtgcaata catggtgaat atgcaataat aatcgaaggc
     2161 cctttataac tttcagcttc tctaaatgct cttaccattt gtgcttggtt tgcgccgtga
     2221 gcaattgatg ctacgtaaac atgaccataa ctcatagcaa ttgcagctag gtccttcttc
     2281 ttaccagttt ttccaccagc agtaaactta gcaattgaac cagtttgact tgctttagaa
     2341 gattgtcctc cagtatttga atatacttca gtatctaata ctaagatatt aacatcttca
     2401 tggttagcaa taacgtggtc taaaccacca aagccgatat catatgccca accatcacca
     2461 ccaataatcc attgactacg tttgattaga tgttcatgca tacctaatac tgctttagct
     2521 tctttagatt tatctttttc aagttctttc attaattctg gatataattc ttttgtctta
     2581 tctccatcat ctttattttc aagccatgct tttgctagtt ctacaagttt atcgtttttg
     2641 cctgcttcaa tcaaagcatt aaattctttt gcaatcttat ctctaataac tccatgagca
     2701 attaccatac cataaccaaa ctcagcgtta tcttcaaata atgagttagc ccatgcaggt
     2761 ccaaatcctt ctttgttgca agtataagga gttgttggat atgatccacc ataaattgat
     2821 gagcatcctg ttgcgtttgc aattaccatt ctatcaccaa ataattgtgt tgcaagtttt
     2881 acataaggcg tttcaccaca accagcacat gcaccactat attcaaatag tggaactgca
     2941 aattgtgagt tctttgcatt tgctttaaca tccattaaat gagctttgtt tgaaacttca
     3001 tgtaagaaga attcagcttt ttcttgcatt ttgttttcaa tttcgccttc aatttcttta
     3061 aatgttagag ccttttcacc acgtcttcct ggacattgtt caatacatac accacaacct
     3121 gtacagtctg caattgaaat tgaaattgca tattgtaatc cttctaatcc tctaccaata
     3181 gcaggaagcg ttacaactcc ttcgggagca ttctttaact cttcttcagt agctaagaat
     3241 ggtctaataa ctgcatgtgg acatacaaat gcacattggt tacattggat acaattttct
     3301 ggtaaccaat gagctacttg agctgctaca tatctttttt catatgctgc agttccagga
     3361 gccatagttc catctgcata tttttcaaaa gcagaaactg gaagtttttc tcctcttaaa
     3421 gcattaattt catcagcaat tcttgctacg aaatcaggtt tcttaactaa ctttttcttt
     3481 tgatctttta gatctttcca agcagctgga acagcaacct tcttaagtcc tttagcacct
     3541 aaatcaattg ccttatggtt catatctaca actgcttgtc ctttactaat atatgaatca
     3601 tgagcagctt gtttcattaa accttttgct ttttcataat ccataatttg cggacttaat
     3661 ttaaagaatg cagattgtaa aatagtgttt gtacgatgac ctaaaccaat cttttgtgca
     3721 gcgtgaatag catcaataat atagaattta atcttctttt ctgccaaagc gcgtttaaat
     3781 ttattaggta agaaatcttc taattcttct ttactacgtg ttgtattaag taagaaagtt
     3841 ccacctttct ttaagttgtc caaaatatca aacttttgta catatgtatc taatgaacaa
     3901 gaaataaagt ctgcttctgg aattaaataa ggtgattgaa ttggatctgg tgagaaacgt
     3961 aaatggctcc ttgtaactcc gcctgacttt ttagagtcat aagcaaaata tgcttgtgca
     4021 taaaagtcag tattttctcc aattaactta attgtatttt tattagcacc tacagtacca
     4081 tctccaccaa taccgtagaa aataaggtta gtagcgttag gtgaaccaac tctaatttct
     4141 tttgtaactg gtaatgataa atgagtaaca tcatctacaa taccaatagt aaattctctt
     4201 ttagcttttt cgcctaattt taagttatca taaacagcaa aaatttgtcc tggagtcgta
     4261 tcttttgaag ataatccata acgaccacca ataatcattg gagcattctt tttgccataa
     4321 tatacagcct taacatctaa agtaagtggt tcatcgtgtg cacctggttc tttagtacga
     4381 tctaaaacag caattctctt tactgatttc ggcatagctt tgaagaaata ttcttcagag
     4441 aaaggacgat ataagtaaac agttaataat ccaacttttt tacctttaga agttaaataa
     4501 tctacaactt ccttaactgt ttgtgttaca gaacccatag ctacaattac atctgttgcc
     4561 tcagggtgtc catagtaaac aaaaggagca tactttcttt tagtttcttt tgacacttct
     4621 tctaaataat ggtttacgat atctggtaaa gcattataat atttatttga tacttctcta
     4681 gtttggaaat atacgtcatc attttgagct gaaccgcgag taactggttt atgaggattt
     4741 aaagcattct ctctaaactt cttaactgcg tccatatcaa tttgacgttt ataaaattca
     4801 taatccataa cttctacagt ttgaatttcg tgacttgttc taaatccatc aaagaagtga
     4861 acaaaaggaa tacttccctt gatagatgct aagtgagcaa ctccacctaa atccataact
     4921 tcttgaactg aaccagtagc aagcattgca aaacctgttt gtcttgcagc cataacgtct
     4981 tggtgatctc caaaaataga taaagcttgt gcagcaattg ctctagcagc tacatgaata
     5041 actcctggca ataattcacc agaaatctta tacatatttg gaatctttaa caataaacct
     5101 tggcttgcag tataagtagt tgttagtgca ccagtttgaa gtgaaccatg aactgctgca
     5161 gcagcaccag cttcagattg catctcaaca actttaacta catttccaaa taaattcttt
     5221 ttacctttat tagcccaatc atctacatgc tctggcatag gtgatgatgg agtaatgggg
     5281 taaataccag ctacttcagt aaatgcatat gatgtatatg cagctgcctc attaccatcc
     5341 atagcctgta aaactttttt cttttctgac atttaatacc tcctaattaa tatctcaatc
     5401 taccttttat tataccataa ataactataa ttgttaagaa aaaagtttaa tttacaaaca
     5461 aattaaactt tcttttctaa tatattaatc attataatat ttagcctgag ctttaaacat
     5521 tttcgcataa tgtccatcta atttcattag ttcataatgc gttccggttt caataatctt
     5581 actaccctca agaacaataa tcttatcaca gaacaaacaa ctagataatc tatgcgaaat
     5641 ataaagacaa gtcttctccc caacaatctc attaaaatca ctatagattt tatcttcaga
     5701 aattacatct aaagcacttg ttggttcatc taacactaca aatgatgcat ctttatataa
     5761 tgtccttgca aaagcaaact tttgtaattc tccgccacta aactcaacac cattcggatc
     5821 aaaccagcga ttaatatatg tattttcttt attaggaaaa gatagtactt tatctaatac
     5881 ctgtgctctt tctaaacaat catataactt ctcgtggttt tcttcatcaa tcataatatt
     5941 gtctttaatt gtaaaagaaa ttactttaaa gtcttggaag acaacagcaa gctgcttcat
     6001 ataactatca taatcataat ctttaatatt aataccatta attaaaatct caccatcatc
     6061 tacatcatag aaacgacaca ttaatttaat aatcgtcgtc ttaccagacc cattaacgcc
     6121 aactaaagac actctttctt tatcatttat tttaaacgat aaatcttgta aaacataatc
     6181 ttcagttctt ggatatttaa aactaacatt tttaaattct atcgtcttaa tactttctaa
     6241 ctttatagta ccatttttga aaatagattc cttttccata aagttaatgt agttttcttg
     6301 ataaatacaa tcacggtcat aagaaaccat cgtaaagacc gcatcatata gttgattact
     6361 taaatttcta actgccgtat ataatgctgt taatgtagca atagttattc caaatttaaa
     6421 atatgaaaat actaaaacca aaactactag caatacccca acaaaataat aattcgtaaa
     6481 ataacctaaa gatgctttag aatatttttt tgtgagttca atatttttat cattaacttc
     6541 tttattgatt ccatcgctat atttatctaa taatggctca ctattataag agcgaagaag
     6601 tttgtgcatt ctaaaattta aaacactaga attataatac cactgttttc ttgatgttct
     6661 aactattgac ttattatgct cttcgtagat atcttctctt tttgtttgaa tgatataacc
     6721 aaaggttgca taaagaataa caatcacaat aaaaatcaaa gtaatatacc aaggaagtcc
     6781 actaaagata attaccccga taatactaat cattgttaga atggactcaa ttattgacct
     6841 caccgttcta acaaaagaat aaataccacc actataagcc cataaacttt gtcttgcaaa
     6901 agctatgtca tctgcaaact ttggatcttc aatattgaaa taatcaattg taacaacttt
     6961 ctcattaaac attgcttcaa ttttctcatc agctttaaaa gcatagtaag tattaatcat
     7021 atctaagatt ttcgtactaa cataagtaaa acctaaaaga agacaatagc taatagcaat
     7081 gataaacaca gccataaaat ccggcttagg ctctaacatt aaattctcaa taattagctt
     7141 tggtaaataa acagtagcaa caccaaaccc agctttaatt aaagcgctaa gtaagatgaa
     7201 aacaattgaa aaaggattaa cctttaatag aattggaaag aaatatttaa tattccttct
     7261 catacgacgg agtcttttca taaagcacct ccctctttat aatacttagc ttgaatatta
     7321 aatagcttct tatactcagt atctttcttc attaactcat tatgactacc agattcaatt
     7381 aaaacaccat catctagtaa caatactcta tcacaaattt ttgtcactgc tagtctatga
     7441 gaaatataaa tagctgttga attaccaata aactcactta gattattata taaattatac
     7501 tccgcaaaag catctaatgc agcagtaggt tcatctaaaa ttactaaagg tgcagcttta
     7561 taaattgctc tagcaattgc taatttttgt acttcaccgc ctgaaagttc aataccagta
     7621 tctgtaaact cacgcgaaag aattgctttt tctttttctg gtaaagagtc tactttactt
     7681 gttaagtcca ttattttaag agtttgtttt atcttctctt catcactttc ctcacgagga
     7741 ctcattgaaa tattttctcc tagttccatt ccaattgtta cattgtcttg aaaaacagga
     7801 gcaattaatt cttgaaaagt ttttctagaa aactggttta tattaacacc attaattaat
     7861 atttctcctt tagtaggttt ataaaaacca cacaagagtt ttactaaagt cgttttacct
     7921 gctccattta aaccaactaa agcaattttt tctcctgctt taattttaaa cgaaacatca
     7981 tttaaagcat atccttcctg attttcataa ctataaaaga catttttaaa ttctatttca
     8041 actttatttg aaggatggtc gctagtttct tttttctcaa aaacattcat ttcataaaat
     8101 tcgcgataat ctttaataat ataactacaa ctcataaaat caccagacga taaaaccata
     8161 acatccaatg accaagcaaa ttctctaact gcagctaaca tataaacaaa tgtagctatt
     8221 aacattcctt ttgttagaac agcataaatt agaaaaccat agattgctaa aagatcaatt
     8281 gcttctaaaa tgttaataaa actaccaact gttgtttctc taattagata aggttttaaa
     8341 attccaaaga aatttgtata agttctatct ctttctttat ttataaattt atccattttg
     8401 taaatcctta aatctttacc aatacttaaa tttctagaaa tgttatctgt ataactaatt
     8461 cttctattaa tattaggaag cttgtctcga tactctgttt gctctttttt acgagcatac
     8521 ttatttaaaa agaacttaat tatagctaac ccaaaaatta tcacacctaa ccaaagatta
     8581 atataaataa taattgtagc cgcaattgcc aaagaaaata tcttcccaat aaactctgct
     8641 gtagatctaa caatagttaa catcccacca ttttgataaa gagctctttt tgctttttcg
     8701 aatgcgtctt gagtttcact actttcaaat ttttcataat ctatttttag actattgcga
     8761 taaaatttct tttgtaaact aactataact ttctcttttc tataagtatc aatatttgac
     8821 tcaaccaaca tttgaagaac cctaactata aaacagatta aagtaacaag catagctttt
     8881 aaaataacat cacgagcatc ttttccccgt tcaatcccat caacaattac cgttaaaata
     8941 tacgggaaaa gaaatacttg gggaatacgt aaaatttcca aaacaaacaa caatggaaca
     9001 taccacttat caaccttgtt aatttgtttc atggaaaaga taatattaga aaaaataccc
     9061 ttatttttta gtttctttac tttttctttt ttagcttttt tcataaccac tccttgtaaa
     9121 catgttttag tcaaaacaaa gaaaaataaa aaactcctat aatcaaggag ttaatttaat
     9181 caatatattt taaatagaaa gaaaaattaa taaatatttt ctcctttaaa ggttattaac
     9241 tagataaact cctaactaca tttttattca tatctatcaa ctcctttcta ttttttattt
     9301 aattttattc taactactaa catattaaca actttataaa tatttgtcaa taagtaaaag
     9361 aaaaagtttg attttaaaat caaacttatt tttctaatat ttttttataa actacgctct
     9421 tagatattcc tctatctttc gcaacttctt tcattgcatc tttatcacta aaccctaaat
     9481 caatataata attataatga tttaagatat ctaattcatt caatttcaaa gctaaactgt
     9541 caattttagc tccttctaaa ataatcgtaa tctctccttt taaatttaaa tcttgcttac
     9601 ttaattcatc taatgtgctt cttatatact cttcatattt cttagtaagt tctcttgcaa
     9661 taactacttt cctatttcca aatacctctg ccattagctt gattgtctca tctaatcttt
     9721 taggagattc ataaaagatt aatgtttctt tattatctac taaagcttct aattctttta
     9781 acttagcgct ttttttatgt tctaaaaatc cataaaatag tgttctatta attggcatcc
     9841 cagaaccaac tacagctgta atataagcag ttgggccagg taaaactgta acttcgatat
     9901 cttcttttat tgcttttgaa ataatcaaat atcctggatc actaattccc ggcatcccag
     9961 catcactaac catcgcaatg tttaatcctt gatttaattt agtaattatc tgttctgaaa
    10021 catcggcttc attaaacata tgataactat aaagttgagt cttaatatta tagtgactaa
    10081 ataaaatttt tgtaactctc gtatcttctg cataaactaa atcaacttca tttaagatat
    10141 ctaaagccct taaagtaatg tctttaatat taccaatagg agttggaacg atatataatt
    10201 tacctttcat ttattcctcc tttttaaaat taaagatttt taaaacttca tctgtatatt
    10261 taccatcttc attatgaaca actaacggtc tttcaacaac aacatcatct ttacgatttt
    10321 tcttagcttc gagtaaaaag attaaagcat cagattctat tttaggataa ataaacttta
    10381 gtctttttac accaaaatta ttctctttta ataaaagtaa aacttcagat aatctaaacg
    10441 tgcggtgaac taaatagaaa ctaccaccat caactaatag ttttgtagct tcctttacaa
    10501 tatcttctaa gcaaactaaa acctcatgtc ttgcaattgt aagttctcta cttttattta
    10561 cattacttcc ttccttgtat ttaaaataag gagggttaga acaaacaata ttaaacttat
    10621 tagcacctat tgttttataa acacctttta gattcttgtt tacaggaatg atttgttttt
    10681 caaaaccatt gatttcaatt gacttaacga aaagctcaca gatttcttct tgaatctcaa
    10741 cgccataaat tttagatttg gtttttaaag tcaaaaataa aggaataggt ccattacctg
    10801 ttcctaaatc tacaattata tctttttctt ttggtttaat aaaatctgct agtaacatcg
    10861 aatctaaaga aaacttaaag tttttatctt tttgatagat tttgatattt tcataaccta
    10921 acagttcatg aagataaagt ttagtcatca atatcatcag ttagatcttc ttctctaact
    10981 tcttcaactt ttcgttttaa actttttact aatgatgcgt taaatgtttc taatttatct
    11041 tcatctttct taactgtaac tagttctctt aaatagtcta catctacaac catacaagat
    11101 tcacaagttg gcgtctttac aatatcacca actcctggaa ttttctttct ttgttcttga
    11161 tagaattcac tttcaaaacc aatacagcac attagtttgc cacacacacc tgcaacttta
    11221 ttggcactta agttcattcc ttgatctttt gccatcttca ttgtaactgg tgaaaactct
    11281 ctaatatgac ttgtacaaca agtctctcta ccacacacac ctaaaccacc aataaactta
    11341 gctccttctc taattgcaac ttgccttagt tcaattctca aacgaaaagc agatgctaag
    11401 tcttttaata attctctaaa gtcaacacga tcttctgcag tgtaataaat aataattttt
    11461 ttattatcta aagtatactc agcatcaagt aacttcattg gcagtttgtt tttgtcaatt
    11521 aattcctgac aaatttctaa tgcttctggt tttcttgcta tattcttttt atattgttca
    11581 acgtcttttt ttgttgctag ccttaaaaca gactttaagt ctttaacttt gttattatca
    11641 acttccatta ctccctgtac aactaaacct atttctactc cccttaccgt ctccacaaca
    11701 acataatcac cctcattgat tgacaaataa tggggattaa accaatagat cttccctaca
    11761 ggtttaaaag ctatccctac aactttaacc atttattttc tccatttcta aaaacaaatc
    11821 aaaaaacatt aaatctatat taatattaaa attaagtttg ttttttgctt ctaataatag
    11881 ctcaatatta gctattatct tttcatattc catttctatt tctccatcaa tattatttag
    11941 taaatcaaaa taaacgatat cttcaatatt acctaattga tacattaaga tatcattgta
    12001 aaaatatact agcatatcta aaaaatactg atggtatttt ctatcttcta atttaaaaaa
    12061 tttctttcct aacaaattta tagtaattat aggagattcg tttcctaaaa gtaaattctt
    12121 aataattttt ttagctaaat cataagtttc taaaaaatca gggtcattag caatatctaa
    12181 acccttttct aaactattag ttaactttac tacaatcttt gcaatatttt caggaacttt
    12241 ttcttctgta agtaaagcta taacatcttt ttctctagct tttttaaaag taataatttg
    12301 gcctcttgat ttaatagttt ctattacgct gcttaaattc tctgtaatta aaacaccata
    12361 atcagttgca cttaattcct ctaaaaactt caataaagaa tttgaagctg atttattagc
    12421 tttttctatt ccctcaataa taaaaactct ttttccttcc tctaaagctg ttaaagaaaa
    12481 ctcgcgaatt aaagcatcaa cttgtggctt agtaatcata ttcttctctg cttttaaatg
    12541 gaaaactgaa gggtgagttc gatctaaaat tctcttacaa tgagcacaat taaaacaagg
    12601 tttctcctga gcctgacaaa aaatcaaagc tgataaataa tacgctgctt ctagtttatg
    12661 cgttccacta ggtccatcaa ataaataaac attaactaga tggtttgtat caacactctt
    12721 atttaaaagt ttagcaactt gaggttgatt ttctaaaaac tcgctaaaat tcatctattt
    12781 atcctttcct ttatttaaag tctctaaaac aagtttataa caacttttat aaacttcttc
    12841 taaagtttga tgaccattta caactttaat tctttcagga tacattttta ctaattcatg
    12901 atatccttca tatactttat tatgaaaagc cattctttct aaatctaaac gattcaaatc
    12961 actactacga ttactttgaa ttctctcaat tccaactttt ggatctaaat caataaaaat
    13021 agttaaatta ggaaaataat catcaattgc ataacgattt atttctaata cttcccttat
    13081 tcctaaacca cgagcatacc cttggtaagc taaggatgag tctacataac gatcacataa
    13141 aacaacgtaa ccatcatcta atttaggctt tacaacttcc attaaatgtt gtcttcttga
    13201 agcagcataa agtaaagctt ctgctttata gtctagttca gtgttcttta catctaaaat
    13261 aacatcacgg atttgttcag cgatcttaga acctccaggt tctcttgtct tataaacttt
    13321 atatcctagt tttattaaat cttgataaat taaattaata atagaagtct tacctgtacc
    13381 ttctccacct tcgaatgtaa taaacataac ttgttctcct tattctatta tattatacct
    13441 taaatgaacc aaaaaataaa cctttttaaa aaaagactaa aatatttatt ttagtctttt
    13501 aatattctac acttactaaa tacaaacctg aaccaggtac taatttaggt gttaactttt
    13561 gatctttgct gtctagtatt tcttttaact tatttactcc aagttttcct tttaaaactt
    13621 ctaaaatcat accaattatt aacctaatca tattgtgtaa aaagccatca cctttaatct
    13681 caaatactaa taaattatca atatttttga ttgtaaaagt atagatagtt cttactgtgt
    13741 tttctttctc atttccttta gagaaagatt taaagtcttt tgttccaata aagatttttg
    13801 gtatttcatc taatttactt aaatctaact ttggtccttt gtaataataa cgataattta
    13861 ctaataaagg attaacttcg tttaaatcta ataaataacg atatgtttta gctttagcat
    13921 taaatctcgc atgaaaatta tcatcaactt tctcaacatt atttacataa atatcttgag
    13981 gta
//
LOCUS       DAOSGS010000012        13664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_3730_length_13664_cov_4.489970, whole genome shotgun sequence.
ACCESSION   DAOSGS010000012 DAOSGS010000000
VERSION     DAOSGS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_13664_cov_4.489970"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..3429
                     /locus_tag="PLQ22_01190"
     CDS             244..3429
                     /locus_tag="PLQ22_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH58607.1"
                     /translation="MKKWLSLLLVFGLSLLLFGCSCDDKVIEVTSVELGANAKELQVG
                     QTYQINPTVKPDDATDKKVTYDSNNKAVATVSASGLVSAVAEGTAVITVKAGAKQAEL
                     TITVLPVEEVQLVITVPQEAATIVVGGTYTLGAQVKDQTGAPQPATLAYASNNADVAT
                     VDDSGKVTGHAAGTAVITITFGELSKTVTITVTAEEPGERAIAGVPEEDPENNVYIFI
                     GRKFTATVENLREGETVAWSTQDTEVISLSAEGAVEALEAGIATIHAIVSDAEGNQVA
                     DLSATFEVMDESILPLFVSEPGYTLKREEIAVEYSASYKTNKVDPVTQDVIYKVEDIT
                     DPENPVELGQNYEANEQEIPAEDIAEVLTFVIAADGTSKTGVNRGRLRVVALKPGLLR
                     ITAKVGDETSEPFELRLIDRPMPTELVISAPNNDPQIETKGGSLKLSVSSKPAFSYSS
                     VTWSSNNPLVDVRSDGTLICNGTDDDIGTVVTITATSTVVEGLTATIDITIKEKFVEE
                     IKTPEPTSITINGYPDVFIGETYQFTADVGPAGANQAVVWSSQNDSYGSVDENGLVTV
                     HAAGEGKSFYIIARAPSVENPGTYAVTQRYQIRAAKPPIDPDPINLNGYQITIMNAGH
                     ALFDQDPFLPAYAGADKTAKQAAWREVEEAYNCTIKVVAYPEEAPWGPARVQWINNNA
                     NNTKGGADYYIVTTTWLNQFSASQALFPVQDLYIRFGKNSMDPASREAATYKGNLFAM
                     SASTYGANYIDRGLFFNLNLLEEVGMENPAQMFMSDRWTHSDFEQWVLDAQVAIGQKG
                     EGYYAISGRPFFYWAGMINVAGIKMVDSASASLNVDGPIQRSVVNVLKRIYMNHQAWD
                     PVPGYDGQTEAFRTEKAIMDGGEMWFVKTDNRWPIDMWSPNDSSGTRYGYVPFPYPDN
                     MTKEQTKVITKGGSIYAMVNHMTHKHAAAGLNDDAVAYEGIYRALNDVFRWTERKQKE
                     AGELVGETELDVRRQNASLRLDDPFSIECVLFYDKRRVMYDPYEEVIGIWATPSAARP
                     AEEGIRQGADYSAVVAECMPAWQQKVIAQFG"
     gene            3542..4348
                     /locus_tag="PLQ22_01195"
     CDS             3542..4348
                     /locus_tag="PLQ22_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58608.1"
                     /translation="MDVEKYVNSKAFIFFEWTYRLIIMNLLNLSIIITMSFGFWVLFI
                     AVQKMTFFYLAILLTLFGLIITSLTSFKTIKDLSGYRKSGLYKTYFSNLWFSFKDLWF
                     IELLILIVYAFLLGSSVTYSLILKHEDFTYDFIGIVYTVGYWLDWVLLFFLTQAVISF
                     WAVYSHFQMTKWNYIKASFYMSFKLFLPTLLAGIFFFLTPVLIIVLGLILNPAWSLLL
                     ISFFSLFGISLPQYLYFRMIKGKYAYLTKNPDDIQDFDKYELEKENKDEI"
     gene            4338..5480
                     /locus_tag="PLQ22_01200"
     CDS             4338..5480
                     /locus_tag="PLQ22_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOH58609.1"
                     /translation="MKFKLGIDRIDEHLDLFKGKRVGLITNPTGINSKYQSTIDVLNE
                     KTNLVALFSPEHGVRGNLQAGINLGTYEDEETGITVYSLYGDTRKPSKEMLDKIDILC
                     IDIQDVGARFYTYVYTMAYSMMACKEEGKKFIVFDRPNPINASDYEGNILDINYRSFI
                     GYYPTIQRHGMTIGELAYLFNKEFEIDCDLEVIKMAGYDRTKYLDELDILYVYPSPNL
                     PTVNSCILYPGTCIFEGTNMSEGRGTATPFELIGAPYINSKELADKLNSMKIEGVHFR
                     PQYFTPIYSKNKDLLCGGVYIHILDRKKLKSVSLGFILLDTIRNLYPNDFKVNAPWRE
                     GGRTMLDLNTGTDKISKYLLTLEEQLTWIKKDTKTFGKTRSKYLIY"
     gene            5492..8113
                     /gene="nagZ"
                     /locus_tag="PLQ22_01205"
     CDS             5492..8113
                     /gene="nagZ"
                     /locus_tag="PLQ22_01205"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOH58610.1"
                     /translation="MKKLHELTLEEKIGQLFIVGFYGEEFNNDLLNLIKNYKIGNVIL
                     FSRNISSVKQLYKLNKDIHTNIDKQLGIMPFITIDQEGGMVTRIFNDATFFPGAMTIG
                     ATDINNAYYVGQKMGDELRALGINFNLAPVLDVNNNPHNPVIGVRSYSSSEKEVASYG
                     INYIKGLQEKGIIATAKHFPGHGDTNIDSHLGLPIIEHSKDRLNKIELYPFKKAIESG
                     LDAIMSAHIIFKAYDSEKPATLSKKVLTDLLKKELGFKGLIVSDCMEMKAIDETVTAP
                     KGVLEGLLAGLDMVFVSQTPAKQMKAVELVKEAVLNGKFPLHLLDEKVEKILKYKAKS
                     YELLKNEFFNKTYNEQEKILLDPVTKAKALEIVSDSLTLVKGNNVDPSLKTLVLAPDP
                     FASTIIEDEFSKNDRSIYDMVRVENNSNLVPYRIKIKPLKEEIDSILDKAQDYKQIVL
                     CTYNATINQGQIELVRKLNNLDKKLYVISMRNPFDILNFKEVPNYLALYEYTPNSIKV
                     LISYLQGKIKPKGNLPVSLKEKTEFGASVYLGLEDYKLEDNLVYLEKLKANGFSFVFV
                     SMHMPEAGSHAYDEFLAIKDKAKSLGLELIVDVSKPTFDKHKMFGVDHIRLDWGFSKE
                     DIVELVLEGYDIELNASTLTKELVEYLKERIDLSLVTVSHNFYPKQYTGLKYEDVLEK
                     NKYLNSLGFKVMGYIASQENKRPPLYLGLPTVEAMRKMPVFLQKQEMELLGFSKICFG
                     DAIIGDKELKELTEEKEYFTVPLVLKKGISEKEKEIINKVHRVRIDQSVYMIRSTKYR
                     GEKGIEPNNTKKRSKYDITIDNDLMKRYSGELNIMLTDLEADKAVNVVGRAILSDFSL
                     DYLKMQIPFVFKVIGEE"
     gene            8113..9030
                     /locus_tag="PLQ22_01210"
     CDS             8113..9030
                     /locus_tag="PLQ22_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOH58611.1"
                     /translation="MKRAIIGVDAGGSSTKLALFLEDGTIVRELKAKAGSSASVKSAL
                     ENVEEGVLKLYEEVKDEYDLRYIVLGLSGLKANSKDSLEKLVSNLKAKTNLDVSLNPD
                     TLLALYSFLKDEYEEGIVVISGTGHASFGLNKNGKSMMIGGWGYMIYEIGSAFSAVQD
                     LIIKSVRHYEEEGKVYPLAQSLIKAIDLSTIDDLRVFMYHNDKKEIASYAYLITDGAK
                     AGDKLALEILDDASKGIVRSIKLLVKHLELKETAVLGFTGEYIKNSKILQELVLEKLQ
                     KEKIKLKYLEGSVDPIYGAYYLAKKKGKI"
     gene            9027..10157
                     /locus_tag="PLQ22_01215"
     CDS             9027..10157
                     /locus_tag="PLQ22_01215"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOH58612.1"
                     /translation="MKVIGLMSGTSLDGVDAALVEIKGKKVQLLNFVTYPYPKEFKAK
                     LKKNLNNDQAKLEEICSLNFELGDYFVYAIDEVLKDKYSYDDIDLVASHGQTIWHNPK
                     GLKGQRASTLQIGEAAVIVAKTNITTVSNFRVKDVALDGEGAPLVPKTDFLLYHNNKK
                     NIVLQNIGGIGNLTYLPKDAKEDEILAFDTGPGNVMIDYFMHKYYGKAYDEDGKKAFK
                     GIIIKPLYDYLMSDKFIKKAPPKSTGREQYNETFFESLVKKFNLDQYSKEDVICTVTN
                     ITVDSIVYNYEKFLKEIDLVVVTGGGSHNKYVVSELGKRLGVEVITGTDYGIENDAKE
                     ALSFAYLGYLSFNYKTGNLKSVTGAKENTILGNITYGRYRHG"
     gene            10150..11061
                     /gene="murQ"
                     /locus_tag="PLQ22_01220"
     CDS             10150..11061
                     /gene="murQ"
                     /locus_tag="PLQ22_01220"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOH58613.1"
                     /translation="MVDLSKLTTEKRNKNTYDIDSLDSLGIIKKINEEDKQIAYEIEK
                     HLEEIALVIDDVVESFLEGGRLIYIGAGTSGRLGILDAVECPPTFSVDFKQVQGLIAG
                     GEKAFIKAVEGAEDNKELAVKDLKAINLSNKDTLIGLSASGRTPYVVSGIEYGNKIGA
                     KTASIATSLNSEIGKIAKKKIEVVTGAEVLTGSTRLKSGTAQKMILNMISTASMIQMG
                     KVYENLMIDVNPTNEKLVARALSIIQEITKVEKEEAVLKLKQYKTVKKTIFHLLTKEE
                     SLEKIDYYLNKNHGHLRKAIASYGKKC"
     gene            11045..11779
                     /locus_tag="PLQ22_01225"
     CDS             11045..11779
                     /locus_tag="PLQ22_01225"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOH58614.1"
                     /translation="MVKSVDLKNGDYTLRGIYSKGESEKVVIMFHGYTGHKVEHNGMF
                     RSLSRILLKEDISSLRFDYYGNFDSDGEFTDFTFDTLFSDARCIIKYAYDLGYKEVIL
                     LGYSMGGALALAVAHEDPRIKKVIVWNPAGNINELVKRRYEIAEKDEKGNTISLNFSL
                     SKAMFDSSFKYEWYKLASQYQGKAYVIKGAKDLAVPLLYNDKYLEVLKNKVSFIIKDG
                     DHGFDNKKDQELLYKKTIEYIKEEES"
     gene            11776..12564
                     /locus_tag="PLQ22_01230"
     CDS             11776..12564
                     /locus_tag="PLQ22_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOH58615.1"
                     /translation="MKIIVCIKQVPASTEVEIDPITGNLIRDGRNAKMNPYDLYAIET
                     ALRIKEFNGAHVATISMGPPSALAVLNESLWMGCDDATIMTDRKFGGADVVATSYTLA
                     QGIKLIGSADLIICGKQTTDGDTAQVGPEIAENLKIPHLCYVDKIIEIKEKSIVVQVD
                     MDSTEEVWEIDYPCLITVNKGIFTPRLPSYRRSKKFKDYEIRQIHFSDMVDQNEAHYG
                     LKGSPTQVERMFPPIKNTDKELWEDTDENLALKLSNKLKEMKVV"
     gene            12566..>13664
                     /locus_tag="PLQ22_01235"
     CDS             12566..>13664
                     /locus_tag="PLQ22_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOH58616.1"
                     /translation="MAKKGIIVVDQKNVNKDLVQDYIKSCPFKAIVAKGDSVDVDQAA
                     CKICRICVKKFADVFKFQEIEEEKINKDEWVGITVFIEHDGNKIHPVSLELIGKSKEL
                     AKVTNHPVYALLFSDDANKFTDTLLAYGVDKVYSYEHKLLGHFNVEYYVEVMADFVSK
                     VKPSTILYGGTAIGRSFAPRVAARLKTGLTADCTILDMKKNTDLVQNRPAFGGNIMAG
                     IICENTRPQMATVRYKIFKMPEKVKPFGVVEKMSLKDIKFKTNMRLVETKEKPKEIDI
                     SEADTIVCIGKPFKTKEQIQMAKEFADLIGAQLACTRPLIEAGVMPATRQIGLSGRTV
                     SPKLLICLGISGAVQFTAGMKGSEVVFAVNND"
BASE COUNT         4974 a         1736 c         2671 g         4283 t
ORIGIN      
        1 attaaaaatt gcaattaaag acttacatga tcgtggatgt acagaacttt taattgattg
       61 gacaggcctt ttagacgttt ataatcgttt aggatttgag gtttggaaga gttatacaac
      121 ttacgataaa aagatttaaa actagatatc gttttcaaaa aactcttgat attttaggta
      181 ttatttagta taataataat gaggtcgtag gacttaaagc aaatcaaaca aggaggcaaa
      241 atcatgaaaa aatggttatc attgctttta gtatttggtt tgagcttatt actatttgga
      301 tgtagctgtg atgacaaagt tatcgaagtt acatcagtag agcttggagc aaatgccaaa
      361 gaattacaag taggacaaac ttatcaaatt aatcctactg taaaaccgga cgatgccact
      421 gataaaaaag tgacttacga cagtaataat aaagcagttg ctactgttag tgctagtggc
      481 ttagtatcag cagttgcgga aggaacagct gttatcaccg ttaaagcagg tgcaaaacaa
      541 gcagagctta ctattactgt attaccggta gaagaagtac aattagttat taccgttcca
      601 caggaagcag caacaattgt tgttggcgga acttacacac ttggagcaca agtgaaagat
      661 caaactggag ctccacaacc agcaacatta gcatatgcat caaacaatgc agatgttgca
      721 acagtagacg atagcggaaa agttactgga catgcagcag gaactgcagt tattacaatc
      781 acatttggtg aactttctaa gactgttaca attacagtta cagcagaaga accaggtgag
      841 cgtgcaatcg caggtgttcc tgaggaagat cctgaaaaca acgtatatat ctttattgga
      901 agaaaattta ctgctactgt agaaaaccta agagaaggtg aaacagttgc ttggagcact
      961 caagatactg aagttatctc attaagcgct gaaggtgctg tagaagctct tgaagcaggt
     1021 attgcaacta ttcacgcaat tgtatctgac gcagaaggaa atcaagtagc tgatttaagt
     1081 gcaacttttg aggttatgga tgaatcaatt ctaccattat tcgtatctga accaggatac
     1141 acattaaaga gagaagagat tgcagttgag tattctgcta gttataaaac taataaagta
     1201 gatccagtta ctcaagatgt aatttacaaa gttgaagata ttacagatcc tgaaaatcca
     1261 gttgaacttg gacaaaacta tgaagctaac gaacaagaaa ttccagccga agatatcgct
     1321 gaagtattaa cattcgttat tgcagcagat ggaacaagta aaactggtgt taatagagga
     1381 agattaagag ttgtagcatt aaaaccaggt ttattaagaa tcacagctaa agttggcgat
     1441 gaaacatcag agccatttga attaagatta attgatagac caatgccaac tgaattagta
     1501 attagcgcac caaacaatga cccacaaatt gaaactaaag gtggaagctt aaaattatca
     1561 gtttcaagta aaccagcatt ctcatatagc tctgttactt ggtcatcaaa taatccttta
     1621 gtagatgttc gttcagatgg tacacttatt tgcaacggaa cagacgacga tattggaaca
     1681 gttgtaacta ttacagctac atcaactgta gttgaaggtt taacagctac aatcgatatt
     1741 acaataaaag aaaaatttgt agaagaaatt aaaacaccag aaccaacaag cattactatt
     1801 aacggttatc cagatgtttt cattggtgaa acatatcaat ttacagctga cgttggacca
     1861 gcaggtgcaa atcaagctgt agtttggtca tcacaaaatg attcttatgg ttctgttgat
     1921 gaaaatggtt tagtaactgt tcatgcggct ggtgaaggaa aatcattcta tatcattgcc
     1981 cgtgcaccaa gtgttgaaaa ccctggtaca tatgcagtta cacaaagata tcaaattaga
     2041 gctgctaagc caccaattga tccagatcca attaacttaa atggatatca aattacaatt
     2101 atgaacgcag gccatgcatt atttgatcaa gatccattct taccagcata tgcaggcgct
     2161 gacaagactg ctaaacaagc agcttggcgt gaagttgaag aagcttataa ctgtacaatt
     2221 aaagttgttg cttatccaga agaagctcct tggggaccag cgagagttca atggattaac
     2281 aacaatgcaa ataatacaaa gggtggagca gactactata tcgtaactac tacatggtta
     2341 aatcaattct ctgcaagcca agcattattc ccagtacaag acttatatat tcgttttgga
     2401 aagaatagta tggaccctgc atcaagagaa gcagctacat ataaaggaaa cttatttgct
     2461 atgagcgctt ctacatatgg agctaactat attgatcgtg gtttattctt caaccttaac
     2521 ttactagaag aagttggaat ggagaaccct gctcaaatgt tcatgagtga tcgttggaca
     2581 catagtgatt tcgaacaatg ggtactagat gcacaagttg caatcggcca aaaaggtgaa
     2641 ggttattatg ctattagtgg tagaccattc ttctactggg caggtatgat taacgtagct
     2701 ggtattaaga tggttgactc tgcaagtgct tcacttaacg ttgatggacc tatccaaaga
     2761 agtgtggtta acgtacttaa gagaatctat atgaaccacc aagcttggga tccagttcct
     2821 ggatatgatg gtcaaactga agcattcaga actgaaaaag caattatgga cggcggagaa
     2881 atgtggtttg taaagactga taaccgttgg ccaattgata tgtggtcacc taatgactca
     2941 tctggcacaa gatatggata cgttccattc ccttatccag ataacatgac aaaagaacaa
     3001 actaaggtta ttactaaggg tggatcaatc tacgctatgg ttaaccatat gactcataaa
     3061 cacgcagctg caggcttaaa cgatgatgca gtagcatatg aaggtattta ccgtgctcta
     3121 aacgatgtat ttagatggac agaaagaaag caaaaagaag ctggagaatt agttggagaa
     3181 actgaattag acgttagacg tcaaaatgct agtttaagac tagatgatcc attctcaatt
     3241 gaatgtgttc tattctatga caaacgtaga gtaatgtatg acccatatga agaagtaatc
     3301 ggaatttggg ctactccatc agctgcaaga ccagctgaag aaggtatccg tcaaggagca
     3361 gactactcag cagttgtagc agaatgtatg ccagcatggc aacaaaaggt tatcgcacaa
     3421 ttcggataga atttaaagat ataaacaaaa ccacacgatt ttatcgtgtg gttttttatt
     3481 tctcttttat tgttatttga ccttaggtat ggtataatat aaagagttta aggagttaat
     3541 tatggatgta gaaaagtatg tcaatagtaa agcttttatc ttttttgagt ggacatatcg
     3601 tttaataata atgaatttat tgaatctttc aataattata actatgagtt ttggcttttg
     3661 ggtgctattt attgccgttc aaaaaatgac ttttttttat ctagcaattc tactaacact
     3721 ttttggatta attattactt cacttactag ttttaaaaca attaaagact taagtgggta
     3781 ccgaaagagt ggtttgtata aaacctattt ttctaatctt tggtttagtt ttaaagatct
     3841 ttggtttata gaactgctta ttttaatcgt ttatgctttc ctattagggt catcagtaac
     3901 ttatagtttg attttaaaac acgaagattt tacttatgat tttataggga tagtttatac
     3961 cgttggttac tggcttgatt gggttttatt atttttctta actcaagctg tgatttcatt
     4021 ttgggctgtc tatagccatt ttcaaatgac aaaatggaat tatattaaag ctagtttcta
     4081 tatgtctttt aagttgtttt taccaacact gttagcgggg attttcttct ttttaacccc
     4141 tgtgttaata attgttttag gtttaattct taatccggct tggtcgctct tacttatttc
     4201 gttttttagc ctatttggaa ttagcttacc tcaatactta tattttagaa tgataaaagg
     4261 caaatatgcc tacctaacaa aaaaccctga tgacattcag gattttgata aatacgaatt
     4321 agaaaaggag aataaagatg aaatttaaat taggaattga tcgtattgat gaacacttag
     4381 atttatttaa aggaaagaga gtaggtttaa ttactaaccc tactggtata aattcaaaat
     4441 atcaatctac cattgatgtt ttaaatgaaa agactaactt agttgctttg tttagccctg
     4501 agcatggtgt aagaggaaac ttacaggcag ggattaattt aggaacttat gaagatgaag
     4561 aaacggggat tactgtttat tcgttatatg gagatacaag aaaaccatca aaagagatgt
     4621 tagacaaaat agatatttta tgtatagata ttcaagatgt tggggcaaga ttctacactt
     4681 atgtttatac gatggcttat tcaatgatgg cttgcaaaga agaaggtaag aagtttatag
     4741 tgtttgatcg tcctaatcca attaatgcat ctgattatga gggtaatatt ctagatataa
     4801 attatcgttc ttttatcggt tactatccaa caatccaacg tcatggaatg acaattggag
     4861 aacttgctta tttatttaat aaagagtttg aaattgattg tgatttagaa gttattaaga
     4921 tggctggtta tgaccgcact aaatatttag atgaattaga cattttatat gtttatccat
     4981 cgcctaattt accaacagtt aatagttgta ttctatatcc aggaacttgt atttttgagg
     5041 gtacaaatat gagtgaagga agaggaacag caactccatt tgaattaatt ggagcacctt
     5101 acattaactc taaggaatta gcagataaat taaatagtat gaagattgaa ggagttcatt
     5161 tcagaccgca atatttcacg ccaatttatt caaaaaataa agatttatta tgtggtggag
     5221 tttatattca cattttagat cgtaagaaat taaagtcagt atcattaggt tttatcttac
     5281 ttgatacaat tagaaactta tatccgaacg attttaaggt aaatgcacct tggagagaag
     5341 gcggaagaac aatgctagat ttaaatactg gtactgataa gatatctaaa tacttactta
     5401 ctttagaaga acaattaact tggattaaaa aagatacgaa aacttttggt aagacaagaa
     5461 gcaaatactt aatttattaa ggagaaaatt gatgaaaaaa ttacatgaat taactttaga
     5521 agaaaaaatt ggccaattat ttatcgttgg tttttatggc gaggagttta ataacgattt
     5581 attaaattta attaaaaatt ataagatcgg aaatgtaatt ttattttctc gtaatatttc
     5641 ttcggtaaaa cagttataca aattaaataa agatatccat acaaatatag ataaacaatt
     5701 aggaattatg ccttttatta caattgacca agaaggagga atggttacta gaatctttaa
     5761 tgatgctaca ttctttccgg gggcaatgac aattggcgca actgatatta acaatgctta
     5821 ttatgtaggc caaaagatgg gggacgagtt aagagcttta ggaattaact ttaacttagc
     5881 tccagtatta gatgtgaata ataatcctca taatcctgta attggtgtta gaagttattc
     5941 aagcagtgaa aaagaggttg ccagttatgg cattaattat attaaaggtt tgcaagaaaa
     6001 aggtattatt gcaactgcga aacactttcc aggtcacgga gacacaaaca ttgatagcca
     6061 tttaggcctg ccaataattg aacattcaaa agatcgctta aataaaatag aactttatcc
     6121 atttaaaaaa gcaattgaat ctggacttga tgctattatg agtgctcata ttatttttaa
     6181 agcctatgat agtgaaaagc cagctacttt atcaaagaaa gttttaactg atcttttaaa
     6241 aaaagaatta ggctttaagg gtttaattgt aagtgattgt atggaaatga aagcaattga
     6301 tgagacagtt acagcaccta aaggtgtttt agaaggttta cttgcagggc ttgatatggt
     6361 ttttgtaagc caaacgccag caaaacaaat gaaagctgtt gagcttgtaa aggaagcagt
     6421 gttgaacgga aagtttcctt tgcatttatt agatgaaaaa gtagaaaaga tattaaaata
     6481 taaagctaaa tcatatgagt tattaaagaa tgagtttttt aataaaactt ataatgagca
     6541 agagaaaatt ttacttgatc cagtaactaa agcaaaagca ttagaaatcg tatcagattc
     6601 actgacacta gtaaaaggaa ataatgtaga tccaagttta aaaactttag ttttagctcc
     6661 agatccattt gcctctacaa tcatcgaaga tgagttttct aaaaacgatc gttcaattta
     6721 cgatatggta agagtagaaa acaattctaa cttagttcct tatcgtatta agattaaacc
     6781 tttaaaagaa gaaattgata gtattttaga caaagcccaa gattataaac aaatagtttt
     6841 atgcacatat aatgctacaa ttaaccaagg ccaaattgaa cttgttagaa aactaaataa
     6901 tttagataaa aagctatatg taattagtat gagaaatcct tttgatattt taaactttaa
     6961 ggaagttcct aattacttag cattatatga atatacgcct aactcaatta aggttttaat
     7021 tagttattta caaggcaaaa ttaaaccaaa agggaacttg cctgtttctt taaaagaaaa
     7081 aactgagttt ggcgcaagtg tttatttagg attagaagat tataaactag aagataattt
     7141 agtttattta gaaaagttaa aggcaaatgg ctttagtttt gtttttgtat caatgcatat
     7201 gcctgaagca ggaagtcatg cttatgatga gtttttggct atcaaagata aagctaagag
     7261 tttaggatta gaattgattg ttgatgtatc gaagccaact tttgataaac ataagatgtt
     7321 tggagttgat catattcgtt tggactgggg ttttagtaag gaagatattg ttgagttagt
     7381 tttagaaggt tatgatattg aacttaatgc ttcaacttta acaaaagaat tagttgaata
     7441 tttaaaagaa agaatagatt tatcattagt aacagttagt cataactttt atccaaagca
     7501 atatactggg ttaaaatacg aagatgtact agagaaaaac aaatatttaa acagcttagg
     7561 ttttaaagtt atgggctata ttgcttccca agaaaacaaa aggcctcctt tatatttagg
     7621 attacctaca gtagaagcta tgagaaagat gccagttttt cttcaaaaac aagaaatgga
     7681 acttttaggt ttttcgaaaa tttgttttgg agatgcgatt attggcgata aagaattaaa
     7741 agagttaact gaagagaaag aatattttac tgtgccttta gttcttaaaa aaggaatttc
     7801 agaaaaagaa aaagaaatta ttaataaagt tcatcgtgtt agaattgatc aatcagtgta
     7861 tatgattcgt agtactaaat atcgcggaga aaaagggatt gaaccgaata atacgaagaa
     7921 aagatcgaag tatgatatta cgattgataa tgatttaatg aaaagatatt ctggcgaatt
     7981 aaatattatg ctaactgatt tagaagctga taaggcagtt aatgtagttg gtagagcaat
     8041 tttgagtgac tttagtttag actatttaaa aatgcaaata ccatttgttt tcaaagttat
     8101 tggagaagaa taatgaaaag agcaattata ggtgttgatg ctggtggctc ttcaacaaaa
     8161 ctagccttgt ttttagaaga cggaacaatt gtaagggagt taaaagctaa ggcaggatct
     8221 agcgcatcag ttaaatcagc tttagagaat gtagaagaag gggtattgaa gttatacgaa
     8281 gaagtaaaag atgagtatga cttaagatat attgttcttg gtttaagtgg acttaaagct
     8341 aattctaaag actctttaga aaaattagtt tccaatttaa aagcaaaaac taatctggat
     8401 gtatccctaa atcctgatac tttgcttgct ttatattcat ttttaaaaga tgaatatgaa
     8461 gaaggaattg tcgttatttc tggtaccggt catgcttctt ttggtttaaa taaaaatggt
     8521 aaatcaatga tgattggtgg ttgggggtat atgatttatg aaatcggtag tgcctttagt
     8581 gctgttcaag atttaattat taaatcagtt aggcattatg aagaagaagg taaagtctat
     8641 cctttagccc aatctttaat aaaagcaatt gatttaagca caatagatga cttaagagta
     8701 tttatgtatc ataatgataa aaaagagatt gcaagttatg cttatttaat tactgatgga
     8761 gctaaagctg gcgataagct agccttagag attttagatg atgcaagtaa aggtattgtt
     8821 aggtcaatta aattacttgt aaagcattta gaattaaaag aaactgctgt tttaggtttt
     8881 actggtgaat atattaaaaa ctctaaaatc ttacaagaac tagtattaga aaaattacag
     8941 aaagagaaga taaagttaaa atatttagaa ggtagtgttg accctattta tggagcatat
     9001 tacttagcta agaagaaagg caaaatatga aagttattgg tttaatgagt gggacatctt
     9061 tagatggagt ggatgctgca ttagtagaaa tcaaaggtaa aaaagttcaa cttttgaatt
     9121 ttgttactta tccttaccct aaagagttta aagctaaact aaaaaagaat ttaaataacg
     9181 atcaagcaaa attagaagaa atctgcagtt tgaattttga acttggagat tattttgttt
     9241 atgccatcga cgaagtttta aaagataaat atagctatga tgatattgat ttagttgcaa
     9301 gccatggtca aacaatttgg cataatccaa aaggattaaa aggccaaaga gcatctacct
     9361 tacaaatagg agaagcagct gtaattgtag ctaagacaaa tatcacaaca gtatctaatt
     9421 ttagagttaa agatgtagcc ttagatggag aaggggctcc acttgttcct aaaactgact
     9481 ttttgcttta tcataacaat aagaaaaaca tcgtcttaca aaatattgga ggcattggta
     9541 acttgacata tttaccgaaa gatgctaagg aagatgaaat tttggctttt gatactggtc
     9601 caggtaatgt aatgattgat tattttatgc acaagtatta tggcaaggct tatgatgaag
     9661 atggaaaaaa agcatttaaa ggaattatta ttaaaccttt gtatgattat ttaatgagtg
     9721 ataaatttat aaaaaaagct ccacctaaga gtacaggtag agagcaatat aatgaaacgt
     9781 tttttgaaag tttagttaag aagttcaatt tagaccaata tagtaaagaa gatgtcattt
     9841 gtacagttac aaatattaca gtagattcta ttgtctataa ctatgaaaaa ttcttaaaag
     9901 aaattgattt agtcgtagtt actggaggag gatcacataa taaatatgta gtatcagagt
     9961 taggcaaaag actaggagtt gaagtaatta caggaactga ctacggaatt gaaaatgatg
    10021 ctaaggaagc tttaagtttt gcttatttag gatatttaag ttttaactat aaaacaggaa
    10081 acttgaagtc agttactgga gctaaagaaa atactatttt aggaaatatt acttacggaa
    10141 ggtatcgcca tggttgattt aagtaaacta acaacagaaa aaagaaataa gaatacttat
    10201 gatattgata gtttagatag tttaggaatt atcaagaaaa tcaatgaaga agataaacaa
    10261 attgcttatg aaattgaaaa gcatttagaa gaaattgctt tagtaattga tgatgtagtg
    10321 gaaagttttc tagaaggtgg aagactaatt tacatcggtg ctggtactag tggaagatta
    10381 ggaattttag atgcagttga atgcccacct acatttagtg tggatttcaa gcaagttcaa
    10441 gggttgattg cagggggaga gaaggcattt atcaaagcgg tagaaggagc tgaagataat
    10501 aaagagttag ctgtaaaaga tttaaaagca attaatttgt ctaataaaga cactttaatc
    10561 ggtttaagtg ctagtggaag aaccccttat gtagtgtctg gcatagaata tggcaataaa
    10621 attggagcca aaacagcctc aattgcaaca tctttaaata gtgaaattgg taagattgct
    10681 aagaagaaaa ttgaagttgt aactggagct gaagttttaa ctggttctac aagattaaaa
    10741 tctggaacag ctcagaagat gattttaaat atgatctcaa ctgctagtat gattcaaatg
    10801 ggtaaagttt atgagaactt aatgattgat gttaacccta caaatgaaaa gttagtagca
    10861 agagcattgt caattattca agagattaca aaagtagaaa aagaagaagc tgttttgaaa
    10921 ttaaaacaat ataaaacagt gaaaaagact atttttcatt tactgactaa ggaagaatct
    10981 ttagaaaaaa tagattatta cttaaataaa aatcatggac atttacgaaa ggcaatagct
    11041 agttatggta aaaagtgttg atttaaaaaa tggtgattat actttaagag gtatatatag
    11101 taaaggcgaa agtgaaaagg tagtaattat gtttcatggc tatactggcc ataaagttga
    11161 acataatgga atgtttcgaa gtttatcaag gattttatta aaagaagata tctcttcttt
    11221 aagatttgac tattatggca attttgatag tgatggtgag tttactgatt ttacctttga
    11281 tacactattt agcgatgcta gatgtattat caaatatgct tatgatttag gttataagga
    11341 agttatttta ttaggttatt caatgggtgg cgctttagct ttagcagttg ctcatgaaga
    11401 tccaagaata aaaaaggtaa tcgtttggaa tcctgcaggt aatattaatg agcttgtgaa
    11461 aagaagatat gagattgctg agaaggatga aaaaggtaat acaattagtt tgaatttttc
    11521 tttatcaaaa gcgatgtttg attccagttt taaatatgag tggtataagc tagcaagtca
    11581 atatcaagga aaagcttatg taattaaagg tgctaaagat ttagctgtgc ctttgttata
    11641 taatgataaa tatttagaag ttttaaaaaa taaggtttct tttattatca aagatggaga
    11701 ccatggtttt gataataaaa aagatcaaga gttattatat aagaaaacaa tagagtacat
    11761 taaggaggaa gaaagttgaa aattatagta tgtattaaac aagtgcctgc aagtactgag
    11821 gtagaaatag atcccattac aggtaactta attagagatg gtcgaaatgc taagatgaac
    11881 ccttatgatt tatatgcgat tgaaacagct ttgagaatta aagagttcaa tggagctcat
    11941 gtagctacaa tctcgatggg gccaccttcg gcactagcag tcttaaatga atctttatgg
    12001 atgggttgtg atgatgctac aatcatgact gatcgtaaat ttgggggagc tgatgttgta
    12061 gcaactagct atactttagc tcaagggatt aaactaattg gctctgctga tttaattatc
    12121 tgtgggaagc aaacaacaga tggtgatact gctcaagtag ggccggaaat agcggagaat
    12181 ttaaagattc ctcatttgtg ttatgtagat aagattatcg aaattaagga gaaatcaatt
    12241 gttgttcaag tagatatgga tagtacggaa gaagtatggg agattgatta tccgtgttta
    12301 attacagtaa ataaaggtat ttttacacca agacttcctt cttatagaag aagcaaaaag
    12361 tttaaagatt atgaaattag acaaattcat tttagtgata tggttgatca aaatgaagca
    12421 cattatggtc taaaaggatc accaacacaa gttgaaagaa tgttcccacc aattaaaaat
    12481 actgataaag aattatggga agatactgat gaaaacttag ctttgaaatt gtctaacaaa
    12541 ttaaaggaaa tgaaggtagt ataagatggc taaaaaagga attatcgtag ttgatcaaaa
    12601 gaatgtaaat aaagatttag ttcaagatta tattaaaagt tgtcctttta aggcgattgt
    12661 agctaaggga gattcagttg atgttgatca agctgcttgt aagatttgcc gtatatgtgt
    12721 taaaaagttt gctgatgttt tcaagtttca agaaattgaa gaagaaaaga ttaataaaga
    12781 tgagtgggta gggattactg tttttatcga acatgatgga aataaaattc acccagtatc
    12841 attagagtta attggaaaat ctaaagagtt agcaaaagta actaatcatc cagtttatgc
    12901 cctacttttt agtgatgatg caaataagtt tactgacact ttattagctt atggagttga
    12961 taaagtatac tcatatgagc ataaactact aggacatttt aacgtagaat actatgtaga
    13021 agtgatggca gattttgttt ctaaggtgaa accttcaaca attctttatg gtggtacagc
    13081 aattggtaga agctttgcac caagagtagc agcgagactt aagactggtt taactgctga
    13141 ctgtacaatt ttggatatga aaaagaatac agatttagtg caaaatcgtc ctgcttttgg
    13201 tggaaacatt atggccggaa ttatttgtga aaacacaaga ccacaaatgg ctacggttcg
    13261 ttataagatc ttcaaaatgc cagaaaaggt taaacctttt ggtgttgttg aaaagatgag
    13321 tttgaaagat attaaattta aaactaatat gagattagtt gaaacaaaag aaaagccaaa
    13381 ggaaattgat atttctgagg cagatacaat cgtatgtatt gggaaaccat ttaaaactaa
    13441 agaacaaatt caaatggcaa aagaatttgc tgatttaatt ggagcgcaac ttgcttgtac
    13501 taggccactt atagaggcag gagttatgcc ggctacaaga caaattggcc taagtggtag
    13561 gacagtttcg cctaaacttc ttatttgttt aggtattagt ggagctgttc aatttactgc
    13621 aggaatgaaa ggtagtgaag tagttttcgc agttaacaat gata
//
LOCUS       DAOSGS010000013        12860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4079_length_12860_cov_4.011870, whole genome shotgun sequence.
ACCESSION   DAOSGS010000013 DAOSGS010000000
VERSION     DAOSGS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_12860_cov_4.011870"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLQ22_01240"
     CDS             complement(<1..619)
                     /locus_tag="PLQ22_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOH58617.1"
                     /translation="MGLFDWFRKNKQKKEESKKYKEGLEKTRKQGSLSRLTRLFNEKD
                     KVDDDLFDQLEEIFVMADMGVETTVKFIDSLRDDERLDDLEDMKLLQEIIVDKMFDLY
                     LKGEIVNSNLNVNKEGLSVYLFVGVNGSGKTTSIAKVASKLKKDGKKVCMAAGDTFRA
                     GAIEQLKIWGDRVGCPVITKEEGSDPSAVIFDGIKFAKENGYDVLL"
     gene            complement(692..2674)
                     /gene="topA"
                     /locus_tag="PLQ22_01245"
     CDS             complement(692..2674)
                     /gene="topA"
                     /locus_tag="PLQ22_01245"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH58618.1"
                     /translation="MEKYLVIVESPAKSKTIEKYLGKQYQVLSSKGHIRDLKRSGYGG
                     YGVDLENNFEPQYSRLRIRFPEIKALKEALKDKSKVYLATDPDREGESIAWHLTEVLE
                     LNEDDYERVEFNEITKKAVLEAFEHPRKIDQDLVHAQETRRILDRIIGFSLSKLLQKK
                     IGSKSAGRVQSVVLKIIVDHEKEIEAFIPEEYWEIFASFRYKKTDFKAKLMTYNNEKI
                     ELKTEEDANNVINNLTGKYLVDDIIEKERRRNPRPPFTTSTFQQEASSKFNFTAKKTM
                     MIAQGLYEGVDLEDERVGLITYMRTDSQRLSNDFMAKAKDYIINNYGKEYYAPYLSKI
                     NETSQDAHEAIRPTNLDYHPEKIKKYLTLDQYKIYTIIYNRALAALMAPALFLDTKVK
                     IDNNGYGFELNGSQLLFKGFLEVYNGDSEDNILPKFTKGEEIKNVTIEPVQNFTKPPA
                     RYTEAKLIKKMEELGIGRPSTYSSIIDTLKMRYYVRLENKSFIPTDQGKLTTEKLDQY
                     FSEIINITYTADMEKHLDDIAEGKLVWYKELAKFYDSYLPLLENADQEMEKKEPVYLD
                     ETCPLCGSKLVIRRSRFGEFAACSAYPKCRYIKQDEEHKPIETGIPCPECGTGFLIER
                     TARRGRTRGQRFYGCSNYPKCKATYSSLEDIKKEKE"
     gene            complement(2762..3367)
                     /locus_tag="PLQ22_01250"
     CDS             complement(2762..3367)
                     /locus_tag="PLQ22_01250"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH58619.1"
                     /translation="MTNKYDEKLHKEGYKVIVGLDEAGRGPMAGPLVVAGVALPEGIV
                     IEDLDDSKKLSAKKREVLSKEIKEKALAYKIIFISEKEVDKINVLEASKKGMIEVLET
                     INIAYDLSLSDAIKLPFENNIALIKGDQISYNIAAASILAKVARDEYMIELDKKYPEY
                     NFKKHKGYVTKEHLELLNKYGPSKVHRKSFKPVTQAKKVAN"
     gene            complement(3364..4194)
                     /gene="ylqF"
                     /locus_tag="PLQ22_01255"
     CDS             complement(3364..4194)
                     /gene="ylqF"
                     /locus_tag="PLQ22_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOH58620.1"
                     /translation="MIQWYPGHMAKATKEIEKNLKLVDIVLELVDARIPLSSRNPELK
                     KMLKKKPSLILLTKSTLADPKDNQRWLNILKSENENVLLVDAITNYNVNNIEKEARRI
                     LADKIKKDEAKGIKRNTIRMMIVGIPNVGKSTLINSLVKRKAQEAYNMPGVTKEVKWI
                     RINENLDLLDTPGVLWPKFDDQEVAKKLALTRAIKDSIYQEQELSVYLLEFLKEYYPD
                     LLKERYDVSLELCNYEILKEIALKRGFFKADDIDYDKAAVAVLADFRNMRIGRITLDK
                     "
     gene            complement(4197..4991)
                     /gene="lepB"
                     /locus_tag="PLQ22_01260"
     CDS             complement(4197..4991)
                     /gene="lepB"
                     /locus_tag="PLQ22_01260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH58621.1"
                     /translation="MDHLEYRKRIDLKSLQFIIASILVMAFVLFKGYRLLVISDVLII
                     VALAISVVIVLLNCIFYFSLWKEFALDIYYKVVDLLATFMIVLAMIVFLTTFLVNTAI
                     VNGDSMSPTIEDDEKILIFPSKRVKRFDIVVLEVYEDNIRASGGYLDKNELLIKRLIG
                     LPGDHLKWEHGFLYVNDEVVDDEYLENHGFRKYNLNLDLIFPQHDYEVTLGDDEYFVL
                     GDNRLSSLDSSNIYGVERTSGNFKKTQIKGVVLYIVDGFKIKEIRG"
     gene            complement(4991..7093)
                     /locus_tag="PLQ22_01265"
     CDS             complement(4991..7093)
                     /locus_tag="PLQ22_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH58622.1"
                     /translation="MKKNFFIPQIEEKEEKKETLEEEKKEKGFTSPAGTRQQNKIVYS
                     SISYGNKGMQYESYRDKDKKTENTEEINRRFLETYGSQSNLIQSERKSQANAAKIPSY
                     LQREDITPEDVKRKREYNLDLTQEGEVLKTIDRSELFKDEVKKEVEPKPDLRTIEREY
                     FEELEPVTELSEEKRPRRSRSEPVIRKDDSDGQSIILEEEIRNQKVREFQTEFEEKTT
                     TKRKHRRYRAPSLDLLAQGRGHLQIDKVASQKQIDTINQTLEEFGISGEVVNYVKGPA
                     VTQFEISLGGGVKVERVQNISRNLQMNLECKSLRIEAPIPGKSTVGIEVPNPVQEIVY
                     LGDMLRDKAFLNDGNPLNVALGIAIDGSPIHLNIKEMPHALIAGTPGSGKSVCLNSII
                     MSIIYKADPSEVKLILIDPKFIEFSNFEDIPHLATPIINESKIAQGSLRWAVDEMENR
                     YLKFKSAKRKNIASYNEYMEETNGEKLPYFVIIIDELADLMAIAGNQVEEYIQRIAQK
                     ARAAGIHLIMATQRPSTDVIKGTVKANILTRIAFAVTSQVDSFTILDKSGAEELLGKG
                     DMLYTDGVNEYRIQGAYVSEKEIIRITKFLNETYEPEYMFDQEDLREKIVQEHMADTD
                     PRNDELFEPVARYVVEMQTGSISSIQSRFKVGFTRAQSLMHGLEMLEIVSENKGTTSR
                     DVLVKTLEELEEKLNGDY"
     gene            7235..8062
                     /locus_tag="PLQ22_01270"
     CDS             7235..8062
                     /locus_tag="PLQ22_01270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH58623.1"
                     /translation="MKKDKWLIVIDIDGTLIKDFTQYDYKSMNYLKKLNKKHDVVIAT
                     GRAYKASEELYNYMELDGPVINFNGSYTHNPHNPKLKIQTTSISKKLILKTIADFLAA
                     GIFINAFTEHNDDVFTYKQDDFLDSFFGKSNRTQKYGPLDKLLKTNQHSCFITTNVGT
                     VPFLLDYAKENLGNEYGIRIWTNNDKYVFSELYNLQASKGLAIRRIVEKFGYDQKKTI
                     AIGDGHNDIEMIEYVNYGVAMKNAHPDLLKVAKYKTDSIDDSGVYTFLKNLENENII"
     gene            complement(8091..10676)
                     /locus_tag="PLQ22_01275"
     CDS             complement(8091..10676)
                     /locus_tag="PLQ22_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH58624.1"
                     /translation="MRLKRILVVLLMLVSVGFLTTHKEIKAEPLAKEEEFRGVWVSPL
                     VTDYLGYLSETQFKSELNTIFDNMEYFNMNSIVFHVRIMNDAFYPSIYSNYSRYHQGV
                     KTFDPLAWAIEEAHNRGIEFHAWLNPYRIQNAGASSEAERKALAAKHPALNAASDYRN
                     ILGTDSQAILNPGSPAVREFLLNVCEELVTNYDVDAIHFDDYFYIAGIGQDATTALDR
                     AEYELYNPNNLSLEDWRREQVDIFIQDLRSLLNNYNLYNGKAVQLGISPSGIYRNGNG
                     QITYDAYGNAITNGSDTRGFSHYDYYLYADTLKWANEGWIDYLIPQSYWSFTQTNVEF
                     PKVMGWWDAALKYKKCKVYSGMGLYMTSYSWANKQQEAYDQVAYVYNLANVGGHCIFA
                     YQNLQAARNNGDLSLVKQNYWDKVTLPIKMDTLNQIYPNDINELNVELIEGGVRLSFA
                     PAIHARRYAIYRSTGPLTYSSDELIAVVGKRTFDDLIYYWDLVDNGVTYNYGVRSLSQ
                     SNTPSSGVQKSTAGANMGEPLTLDPITKIDVKGNVYQNEKIEIEVLKVPTLGGVPLEY
                     QVLVSTDGNTYYDPVTTGNNRYDLPQTVVQEFTIPSRNDFYVKVLASNNIATTQSEPF
                     LIDVKENLGSMTIRTLEQDLYYGEVITFEWNHIPGISNYKLQTSTNNVDFEDVGDEYP
                     VVIGPHKCHQQFLVPDYVNILYVRVLGIEGDGEAKSNVLQLTFRENLGKIEGVLINNS
                     NDYYNIVVNEGDQITVSWDVTGYGGDYVIRYSYDGVNFTKTIRDYDRYASAITIVGDC
                     ASQTFSANWEYKVYIIIEEAIGNATVKSDIYWIHTVPCMHLFIEDLMKYMFNENKIAV
                     EAAYN"
     gene            complement(10673..11449)
                     /locus_tag="PLQ22_01280"
     CDS             complement(10673..11449)
                     /locus_tag="PLQ22_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH58625.1"
                     /translation="MAQDIRIISIEKKRKKYKIETNLDTHTLEEDIIIKYNIFKDKVF
                     KEEEFKKILEENKNVASFNQVLNFLSYRARSISEVKEYLEKKEVSKKNSESIVQRLID
                     LDYLNDEAFASYYLGYYQKMNKGPNYIKTKLFEKKVDKDTISKCLLEYTLEDEIEIIA
                     LVLEKIKDKYLKFPIKKQKEKIQAKLMRDGFTFSAVNSALRDLKLEENISETLEKDFG
                     KLKKKYQDLDQKEKKDKIIQNLLRKGYEYSSIKKKVEEEL"
     gene            complement(11433..12359)
                     /locus_tag="PLQ22_01285"
     CDS             complement(11433..12359)
                     /locus_tag="PLQ22_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH58626.1"
                     /translation="MLKKLNHEKRRILKEWVMIVIGAALVAFPFSFLLYPNDWVIGGV
                     GSIGVILRAILPTDSLTPEQITLIPSLVTFLINVILVILAIIFLGKKMILRIIVGSLI
                     FPGFTALFTLFYNALGADFHQKVIELDPILVMLYSSIIMGIGLGTVVKNNGTTGGTEI
                     PQSIFFKYFHMPYSVSLIIIDGTILLLGYIVLQDLSMTLYAVIVVILSGLCMDIIVFG
                     GFNKRAMYIISNKTEEIIELIQKEYNRGVTSLKAIGEYAKTDRKVIMAVVSSKEYNKL
                     RTDIVKIDDKAFFYVVRASEVSGAGFTDGPRH"
     gene            complement(12364..>12860)
                     /locus_tag="PLQ22_01290"
     CDS             complement(12364..>12860)
                     /locus_tag="PLQ22_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOH58627.1"
                     /translation="FDSNARRHTSFCSCSVTPKMTDFEEIEIKPEEIRVDTYRSSGAG
                     GQSVNTTDSAVRITHLETGIVVQCQNERSQIQNREKALEVLKAKLHQLNEEKRQEKLD
                     QLIDISINSFGGQIRSYVYHPYRMVKDHRTNYEELENSANDVLNGNLDGFINSFLKSK
                     FNVR"
BASE COUNT         4207 a         2368 c         1470 g         4815 t
ORIGIN      
        1 aaagtaatac atcataacca ttttccttgg caaacttaat tccatcaaag ataacagctg
       61 atgggtctga accttcttct ttagtaatta caggacagcc tacacggtct ccccaaattt
      121 ttaattgttc aatagcacca gctctaaaag tatctccggc tgccatacaa acttttttac
      181 catctttctt tagttttgat gcaaccttgg caatcgatgt tgtctttccc gaaccattta
      241 cccctacaaa caaataaaca gataatcctt ctttatttac atttaagtta gaatttacaa
      301 tctcaccctt taaatataaa tcaaacattt tgtctactat tatctcttga agaagtttca
      361 tatcttctaa atcatctaat ctttcatcat ctcttaggct atcaataaac ttaacagtag
      421 tctcaacacc catatcagcc attacaaaga tttcttctag ttgatcaaat aaatcatcat
      481 ctactttgtc tttctcatta aatagtctag ttaatcgtga tagtgatcct tgctttcttg
      541 tcttttctag accttcttta tatttttttg attcttcttt tttctgtttg ttttttctaa
      601 accaatcaaa taagcccata ataagcctcc ttgtcattta atattatacc atatttacct
      661 tatttttcat agtaaaaaag aatagcctta actattcttt ttctttctta atatcttcta
      721 aactgctata agtagcctta cattttggat aattagaaca accataaaac ctttgacccc
      781 tagttcttcc acgtcttgca gttctttcaa ttaagaaccc cgtaccacac tctgggcatg
      841 gaataccagt ttcaattggt ttatgttctt catcttgttt gatataccta cattttggat
      901 aagcagagca agctgcaaac tccccaaatc tacttcttct aattacaagc ttacttccac
      961 acaaaggaca agtttcatct aaataaactg gttccttttt ctccatttct tgatctgcat
     1021 tttcaagaag tggtaaataa ctatcataaa acttagctaa ttctttatac caaacaagtt
     1081 taccttctgc gatatcatct aaatgttttt ccatatctgc agtataagta atattaataa
     1141 tctcagaaaa atattgatcc aacttttctg ttgttagttt tccttgatct gtaggtataa
     1201 aagatttatt ttctagtctt acataatatc tcatctttaa agtatcaata atacttgagt
     1261 atgtagatgg gcggccaatt cctaattctt ccatcttttt gattaattta gcttctgtat
     1321 atcttgcagg tggtttagta aagttttgga caggttcaat agttacattt ttaatttctt
     1381 cacctttagt aaatttaggt aaaatattat cttcactatc tccattataa acttctaaaa
     1441 aacctttaaa aagtagctga ctaccattaa gttcaaaacc ataaccatta ttatctatct
     1501 ttacctttgt atctaagaat aaagctggcg ccattaaagc agctaatgct ctattataga
     1561 taatagtata gattttgtat tgatcaagtg ttaagtactt tttgattttc tctggatgat
     1621 agtctaaatt agtcggtcta atagcctcat gtgcatcttg gcttgtttcg tttatcttag
     1681 ataggtaagg cgcataatac tctttgccat aattattaat aatataatct ttagccttag
     1741 ccataaaatc attagataat ctttgagagt ctgtacgcat ataagtaatc aaaccaactc
     1801 tttcatcttc taagtcaaca ccttcatata atccttgagc tatcatcatc gtttttttcg
     1861 ctgtaaagtt aaacttgctt gatgcttctt gttggaaagt tgaggttgta aaaggaggtc
     1921 ttggatttct ccttctttcc ttttcaataa tatcatctac taaatactta ccagttaagt
     1981 tattaataac attatttgca tcttcttcag tctttagttc aatcttttca ttattgtaag
     2041 tcattaactt agctttaaag tcagttttct tataacgaaa actagcaaag atttcccaat
     2101 attcttctgg aataaaagct tcaatttcct tttcgtgatc aacaataatc ttcaaaacta
     2161 cagattgaac tctaccagct gatttagaac ctattttctt ttgtaatagt ttacttaaac
     2221 taaagccaat aatcctatct aaaatccttc ttgtctcttg cgcatgaact aaatcttgat
     2281 caatctttct tggatgttcg aaagcttcta aaacagcctt tttcgtaatt tcattaaatt
     2341 caactctttc atagtcatct tcatttaatt ctaaaacttc agttaaatgc caagcaattg
     2401 attcaccctc acgatcaggg tcggtagcta gataaacttt agatttatct ttcaaagctt
     2461 cctttaaggc tttgatttct ggaaatctaa ttcttagtct actgtattgt ggctcaaaat
     2521 tgttttctaa gtcaacacca taaccaccat atcctgatct cttcaaatca cggatatgcc
     2581 ctttactaga tagaacttga tactgtttac ctaaatattt ttcaattgtc tttgacttag
     2641 ctggactctc cacaatcact aagtattttt ccattattaa tctctcctat ttttacgctt
     2701 tgattatatc attatattat caagattaca aacattttgt tttttttatt ttaaaaagta
     2761 gctaattagc tacttttttt gcttgggtta caggtttaaa actcttgcga tgaaccttag
     2821 aaggcccata tttattcaat aattctaaat gttctttagt tacataaccc ttatgttttt
     2881 taaaattata ttctggatac tttttatcaa gttcaatcat atactcatct cttgcaactt
     2941 tagctagaat actagcagct gcaatattat aactaatttg atcaccttta attaaagcaa
     3001 tattgttttc aaaaggaagt ttaatagcat cacttaaaga taaatcataa gcaatattta
     3061 ttgtttctaa tacttcaatc attccttttt tacttgcttc taaaacattg attttatcta
     3121 cttctttttc agaaataaaa ataattttat aagccaaggc tttttcttta atctctttac
     3181 tcaaaacttc tcttttttta gctgaaagtt tcttagagtc atctaagtct tcaataacaa
     3241 tcccttcagg caaagctaca ccagctacaa ctaaaggtcc agccattgga cctctacctg
     3301 cttcatctaa accaacaatt actttataac cttctttatg aagcttttca tcatatttat
     3361 ttgtcattta tctaaggtaa ttcttcctat tctcatattt ctaaaatcag ctaaaactgc
     3421 tacagcagcc ttatcataat caatatcatc agctttaaag aaacctcttt ttaaggcaat
     3481 ctcttttaag atttcatagt tacaaagttc taaactaaca tcatatcttt cttttaataa
     3541 atcaggataa tattctttta aaaactctag taaataaaca cttaactctt gttcttgata
     3601 aatagaatct ttaattgctc tagttaaggc taatttctta gccacctctt ggtcatcaaa
     3661 ctttggccat aaaacgccag gagtatcaag taaatctaaa ttctcattga ttctaatcca
     3721 ctttacttct ttagtaactc caggcatatt atatgcttct tgagcttttc ttttcactaa
     3781 actattaatt aaagtgctct taccaacatt aggtatccct acaatcatca ttctaatcgt
     3841 attcctttta attcctttag cttcatcttt cttaatttta tcagctaata tccttcttgc
     3901 ttctttttcg atgttattta cgttataatt tgtaattgca tctactaaca aaacattctc
     3961 attttcgctt tttaaaatat taagccatct ttgattatct tttggatcag ctaatgttga
     4021 tttcgtaagt aaaattaaag aaggcttttt cttaagcatt ttctttaact ctgggtttct
     4081 tgaagataaa ggtatccttg catctacaag ttccaaaaca atatcaacta attttaaatt
     4141 cttttcaatt tcttttgtag ctttagccat atggcctgga taccattgaa tcattattac
     4201 cccctaattt cttttatttt aaaaccatct acaatataca aaacaacacc cttaatttgt
     4261 gtttttttga aattaccaga agttctttca actccataaa tattactact atctaaacta
     4321 cttaaacgat tatcacctaa aacaaaatat tcatcatcac ctaaagttac ttcataatca
     4381 tgttgaggaa aaatcagatc aagatttaag ttgtattttc taaaaccatg attttctaaa
     4441 tactcatcat caactacttc atcattaaca taaagaaaac catgttccca tttcaaatga
     4501 tctccaggaa gaccaattaa tctttttatt aataactcat ttttatctaa ataaccgcct
     4561 gatgccctaa tattatcttc atatacttct aacacaacaa tatcaaatct tttaactctt
     4621 ttacttggaa aaattaaaat tttctcatca tcttctattg ttggactcat tgaatctcca
     4681 tttacgatag ctgtatttac aagaaatgtt gttagaaaaa caatcattgc taaaacaatc
     4741 ataaatgtag ctaaaagatc cacaacttta taataaatat ctaaagcaaa ttctttccat
     4801 aaagaaaaat agaaaataca atttaagaga actattacta ctgaaatagc taaagccaca
     4861 attattaaaa catcactaat tactaataac ctataacctt taaataaaac aaaagccatt
     4921 actaaaattg aagctattat aaattgtaat gatttaagat caattctttt tctatattca
     4981 agatgatcca ttaataatct ccgtttagct tttcttctaa ttcttctaaa gtcttaacta
     5041 agacatccct tgatgtagtt cctttatttt ctgacactat ttctagcatt tctaaaccat
     5101 gcattaggct ttgagcacga gtaaaaccaa ctttaaaacg tgattgaata gaactaattg
     5161 aacctgtttg catctccaca acatacctag caactggttc aaacaattca tcatttcttg
     5221 gatcagtatc agccatatgt tcttgaacga tcttttctct taaatcttct tggtcaaaca
     5281 tatattcagg ttcatatgtt tcatttaaga acttagtaat acgaataatt tctttttctg
     5341 atacataagc tccttgaatt ctatattcat tcacgccatc agtataaagc atatcacctt
     5401 taccaagcaa ttcttctgct cctgatttat ctaaaatcgt aaatgaatct acttgagatg
     5461 taactgcaaa agcaattctt gttagaatat tagcttttac tgtacccttt ataacgtcag
     5521 ttgatggtct ttgagtagcc ataattaaat ggatgcctgc agcacgtgct ttttgggcaa
     5581 ttctttgaat atattcttca acttgattac ctgcaatcgc cattaagtct gcaagctcat
     5641 caatgataat tacaaagtaa ggtaacttct ctccatttgt ctcttccata tattcattgt
     5701 aacttgcaat attctttctt tttgcactct taaatttcaa ataacgattt tccatttcat
     5761 caactgccca ccttaaacta ccttgagcaa tcttagactc attaataatt ggtgtcgcta
     5821 aatgagggat atcttcaaaa ttagaaaact cgataaactt aggatcaatt aaaattaact
     5881 taacttcact tggatcagct ttataaataa tactcataat aattgagtta agacaaacac
     5941 tcttacctga tcctggagta cctgcaatta aagcatgtgg catttcttta atattcaaat
     6001 gaattggact accatcaatc gcaataccta aagctacatt taaaggatta ccatcattta
     6061 agaatgcttt atcacgcaac atatcaccta aataaacaat ctcttgaact ggattaggaa
     6121 cctcaatacc aactgtactt ttacctggaa ttggagcttc aattcttaaa gatttacatt
     6181 ccaaattcat ttgtaagtta cgagaaatat tttgaactct ttcaacttta actccaccac
     6241 ctaaagaaat ctcaaattgc gtaacagcag gtcccttcac ataattaact acttcacctg
     6301 aaattccaaa ttcttctaaa gtttggttaa ttgtatcaat ttgtttttgg gatgctactt
     6361 tatcaatttg caaatgtcca cgtccttgag ctaaaaggtc taaactagga gcacgataac
     6421 gacgatgttt tcttttcgta gttgttttct cttcaaattc agtttgaaac tcacgaactt
     6481 tttgatttct aatttcttct tctaaaataa tcgattgtcc atcagaatca tcttttctaa
     6541 tgactggttc tgatcttgaa cgtctaggtc ttttctcttc agaaagttca gttacgggtt
     6601 ctaactcttc aaaatattct ctttcaatag ttcttaagtc cggttttggt tctacttctt
     6661 tcttaacttc atctttaaaa agctcagaac gatctattgt ctttaaaacc tcaccttctt
     6721 gagttagatc taaattatat tctctttttc tcttcacatc ctcaggagta atatcttctc
     6781 tttgcaaata agatgggatc ttagcagcat ttgcttgact ctttctttcg ctttgaatta
     6841 gattagactg actaccataa gtttctaaaa atctacggtt aatttcttct gtattttcag
     6901 tcttcttatc tttatctcga tagctttcat actgcattcc tttattacca taacttattg
     6961 aagaataaac aattttgttt tgttggcgag taccagctgg tgacgtaaaa cctttctctt
     7021 tcttttcttc ttctaaagtt tcttttttct cttctttttc ttcaatttga ggaataaaaa
     7081 aattcttttt catagatata tcctttcttt aattttctgc tcttattata ttataataca
     7141 aatagtcctt tttttcaatc tcaacttaat gataactttt tcatttttgt tttttaataa
     7201 aagtatgata taattacccc aagcaggtaa aaatatgaaa aaagacaaat ggttaattgt
     7261 aattgatatt gatggaactt tgattaaaga ctttacacaa tacgattata aaagtatgaa
     7321 ttacttaaaa aaactaaata aaaaacatga tgtagtaatt gcaacaggaa gagcatataa
     7381 agctagtgaa gaattataca actatatgga actagatggt ccagtaatta actttaatgg
     7441 ctcttatact cacaatcctc ataacccaaa actaaaaatc caaactacat ctatttctaa
     7501 aaagctaatt ttaaaaacaa tagctgattt tttggcggct ggaatattta ttaatgcttt
     7561 tactgaacac aatgatgatg ttttcactta caaacaagat gactttttag attcattttt
     7621 cggaaaatca aaccgtacac aaaaatatgg tccattagat aaacttctaa aaactaacca
     7681 acatagttgt ttcataacta caaatgttgg gacagttcct tttttacttg actatgcaaa
     7741 agagaactta ggcaatgaat atggcattag aatttggact aacaatgata aatatgtttt
     7801 ttcagaacta tataatcttc aagcatctaa aggcctagca attagaagga ttgttgaaaa
     7861 gtttggttat gaccaaaaga aaacaatcgc gattggcgat ggccacaatg atattgaaat
     7921 gattgaatat gttaattatg gtgttgctat gaaaaatgcc cacccagatc ttttaaaagt
     7981 tgcaaaatat aagacagatt caattgatga tagtggtgtt tatacttttt taaaaaactt
     8041 agaaaatgaa aatatcattt aaaaatcccc taaataaggg gattttttta ttagttatat
     8101 gcagcttcca ctgcaatttt gttttcatta aacatatatt tcattaaatc ttcaataaat
     8161 aaatgcatac aaggtacagt atgaatccaa taaatatctg acttaacagt tgcattacca
     8221 attgcttctt caatgataat atagacttta tattcccagt tagcagaaaa tgtttgacta
     8281 gcacaatcac ccacaatagt tatagccgaa gcatagcgat cataatctct aatagttttt
     8341 gtaaagttaa caccatcata tgaataacga attacataat ctccgccata acctgtaaca
     8401 tcccaagaaa cagtgatttg atctccttca tttactacga tattataata atcattagaa
     8461 ttattaatta atactccttc aatcttacct aaattttctc taaaagtaag ttgtaaaaca
     8521 tttgattttg cttctccatc tccttcaatt cctaaaactc tcacataaag aatattaaca
     8581 tagtcaggaa ctaagaattg ttggtggcat ttatgaggtc caattacaac aggatattca
     8641 tccccaacat cttcaaaatc tacgttgttt gtagaagttt gaagtttata attagaaata
     8701 ccaggaatat gattccactc aaaagttatt acttcaccat agtaaagatc ttgttctaac
     8761 gttcttatcg tcatagaacc taagttttct ttcacatcaa ttaaaaatgg ttctgactga
     8821 gttgtagcta tattatttga cgcaagaacc ttgacataaa agtcatttcg acttggaatc
     8881 gtaaattctt gaacaacagt ttgaggcaaa tcataacgat tattaccagt agttactgga
     8941 tcataatatg tattaccatc agttgaaact aatacttgat attctaacgg aactcctcca
     9001 agtgtaggaa cctttaaaac ttcaatttca attttctcat tttgataaac gttccctttt
     9061 acatcaattt tagtaatagg atctaaagtt agtggttctc ccatatttgc accagctgta
     9121 ctcttttgta ctccactact tggtgtatta gattgtgata aacttcttac tccataatta
     9181 taagttactc cattatcaac caaatcccaa taataaatta aatcatcaaa tgttctttta
     9241 ccaacaacag cgattaattc atcacttgaa tatgtaagag ggcctgtact acggtaaatg
     9301 gcatatcttc ttgcatgtat tgcaggagca aagcttaatc ttactccacc ctcaattaac
     9361 tctacattta attcattaat atcgttagga taaatttgat ttaaagtatc catctttata
     9421 ggtaaagtta ctttatccca atagttttgc tttactaagc tcaaatcacc attatttcta
     9481 gctgcttgta agttttgata agcgaaaatg caatgtcccc caacatttgc taagttgtat
     9541 acatatgcta cttgatcata tgcttcttgt tgtttattag cccaagaata actagtcata
     9601 tataatccca ttccagaata tactttacat tttttatatt ttaaagcagc atcccaccaa
     9661 cccattactt taggaaactc tacatttgtt tgagtgaaac tccagtaact ttgaggaatt
     9721 aaataatcaa tccaaccttc attagcccat tttaaagtat ctgcatataa gtaataatca
     9781 taatgagaaa aacctcttgt atctgagcca ttagtaatag catttccata tgcatcataa
     9841 gttatttgtc cattgccatt acgataaatt cctgatggag aaatacctaa ttgaactgct
     9901 ttgccattat aaaggttata attatttaat agactacgca agtcttgaat aaaaatatct
     9961 acttgttctc ttctccagtc ttctaagctc aaattattag gattatataa ttcatattct
    10021 gctctatcta aagcagttgt agcatcttgt cctataccag caatataaaa ataatcatca
    10081 aagtgaattg catctacatc ataattagtt actaattcct cacaaacatt aagcaaaaac
    10141 tctctaactg caggacttcc tggatttaga attgcttgtg agtctgtacc taaaatatta
    10201 cgataatcac ttgcggcatt taaagctgga tgtttagcgg ctaaagcttt tctttcagcc
    10261 tcacttgaag cacctgcatt ttgaatacga taaggattaa gccaagcatg aaattcaatt
    10321 cccctattat gagcttcttc aatagcccaa gctaaaggat caaaagtttt tacaccttgg
    10381 tgataacgtg aatagttact atatatagat ggataaaaag catcattcat aattctaaca
    10441 tgaaatacaa ttgagttcat attaaagtat tccatattat caaaaattgt atttaactca
    10501 cttttaaatt gggtttcact taaatatccc aaataatctg taactaaagg agaaacccaa
    10561 actcctctaa attcttcttc ttttgcaagt ggttcagctt ttatttcttt atgcgttgta
    10621 agaaagccca ctgacacaag cattaataat actactagta tcctttttag tctcataatt
    10681 cctcctctac cttcttttta atactactat attcatatcc ctttcttaat aagttttgaa
    10741 taattttatc ttttttctct ttttgatcta aatcttgata tttctttttt aattttccaa
    10801 aatctttttc taatgtttca ctaatatttt cttctaattt taaatctctt aaagcactat
    10861 ttactgcact aaaagtaaat ccatctctca ttaatttagc ttgaattttc tctttttgct
    10921 tttttattgg aaacttaaga tatttatctt taatcttttc taaaactaaa gcaataatct
    10981 caatttcatc ttctaaagta tattcaagca agcatttaga aatagtatct ttatcaactt
    11041 tcttttcaaa aagtttagtt ttaatatagt taggaccttt attcattttt tgataatagc
    11101 ctaaataata agaagcgaaa gcttcgtcat tcaaataatc taaatcaatt agtctttgaa
    11161 caatactttc gctattcttt ttactaactt cttttttttc taaatattct tttacttcag
    11221 aaattgatct agctctatat gacaagaaat ttaaaacttg attaaaacta gcaacatttt
    11281 tattttcttc taagatcttt ttaaattctt cttctttaaa tactttatct ttgaaaatat
    11341 tatatttaat aataatatct tcttctaaag tatgagtatc taaattagtc tcaattttat
    11401 atttttttct tttcttctca atagaaataa ttctaatgtc ttgggccatc ggtgaatccc
    11461 gctcctgaaa cttcgcttgc cctaactaca tagaagaaag ctttatcatc aatcttgaca
    11521 atgtctgttc ttaatttatt gtattcttta gaactaacta cagccataat aactttgcga
    11581 tcagttttcg catattcacc aatagctttt aagcttgtaa ctccgcgatt atattctttt
    11641 tgaattagtt caataatttc ttcagtttta ttggaaataa tgtacatcgc tcttttatta
    11701 aatccaccaa agacgataat atccatacat aaaccactca agataactac aataactgca
    11761 tataaagtca ttgataaatc ttgtaaaaca atataaccaa gtaacaaaat tgttccatca
    11821 ataataatta atgatactga ataaggcata tgaaaatatt taaagaaaat agattgggga
    11881 atctctgttc cacctgtagt accattattc tttacaactg ttccaagtcc tattcccata
    11941 ataattgatg aataaagcat aactaaaatt ggatctagtt cgataacttt ttggtgaaaa
    12001 tcagctccta aagcattata aaataaagta aacaaagctg tgaaaccagg aaatattaat
    12061 gacccaacaa ttattcttaa aatcattttt ttacctaaga aaataatggc taagattact
    12121 aatataacgt ttatcaaaaa ggttactaat gatggaataa gagttatctg ttctggagtt
    12181 aaactatcag ttggtaatat tgctcttaaa ataacaccaa tacttccaac tccaccaata
    12241 acccaatcat taggatatag tagaaaacta aatggaaatg ccacaagagc agctccaata
    12301 acaatcataa cccattcttt taaaattctt cgtttttcat gatttaactt ttttagcatt
    12361 tttttacctc acattaaatt tactttttaa aaagctattg ataaacccat ctagattacc
    12421 atttagaaca tcattagcgc tattctctaa ttcttcataa ttagtacgat ggtcttttac
    12481 catcctatat ggatgataaa cgtatgatct gatttgaccg ccaaaactat ttattgaaat
    12541 atcaattaat tgatctaatt tttcttggcg tttttcttca tttagttgat gtagtttagc
    12601 ttttaaaact tctaaagctt tttctctatt ttgaatttgg cttctttcat tttggcattg
    12661 tactactatc cctgtttcta aatgcgtaat tctaactgca gaatcagttg tgtttacaga
    12721 ttgtccccca gccccactag agcgataagt atcaactcta atttcttctg gcttaatttc
    12781 tatttcttca aaatcagtca ttttcggagt tacactgcac gaacaaaacg atgtatgacg
    12841 acgagcatta ctatcaaatg
//
LOCUS       DAOSGS010000014        12709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4147_length_12709_cov_5.744903, whole genome shotgun sequence.
ACCESSION   DAOSGS010000014 DAOSGS010000000
VERSION     DAOSGS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12709
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4147_length_12709_cov_5.744903"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PLQ22_01295"
     CDS             <1..1558
                     /locus_tag="PLQ22_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOH58628.1"
                     /translation="IDKFFSEPGNMIIKGLVIGDSKSFTLDFASDLRINGTSHLFAIS
                     GAHVNIITYFLIKVFARSKYQDKIVSSLLIIYLFLTNFLPSVLRAVIMYLLHILNKKY
                     QLHFKTLDLLAIAFILMIIINPFYFFNLSFQLSFIVTAIIILLASLIKDKSSLIQTVI
                     ISTAAVIATLPIIANINSEVNLLSPLVNVVFIFLVLEILLPLSFIILVLPFLSKFYNL
                     LVQGFIFLNNLFASQIKISVPLPYFKIYHSLIYYSFFIVFFSLNKFKSRKIVLSFFLI
                     LFSFFILFRSYTTSLKVTFLDLYNGESILIQKGVSKVLIDTGAGQGKKVSSFLRKSGI
                     YHLDYLIITHDHFDHYGEAKDICDVVNVKRVITSSISKLSFQRQIKIGSPYIIKLGKD
                     ELILYPPEKQYLDENNNSIITFLKSELKFLFLGDLVMTGEERLNGFKEKIDVLKVGHH
                     GSQTATSPNLLSILKPKYAIIQTGRVKSFGFPDHELINRLNNYQIKTYRTDLHYTIVY
                     QNKKFTYLNQ"
     gene            1603..2562
                     /gene="holA"
                     /locus_tag="PLQ22_01300"
     CDS             1603..2562
                     /gene="holA"
                     /locus_tag="PLQ22_01300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH58629.1"
                     /translation="MAKVYLFLGNEEYLNKVKIERIIKESVADEYNINYYDMEEKNVS
                     FAVEDAQTAPFLCEEKIVVLRHPKFLTTAKLEIEHDIKGFVKYLNNPSPYTIFIIDAS
                     NLKLDNRKEVVKVLLKVAIKEESESLSDVEFVGWVIRQFSQNNLKIGQRAAQTFLSRT
                     GTNLINASNEIDKLLLYCMDKDEVTEEDVINLTTKELEQDIFSFTNAIMEKNKMKAIE
                     VYRDLVATNKFEPVQLLITSSNNLKDCYLVKLLMEKNYKQNQIAESLGVSPGRAYYLM
                     RDARSLDKERAAELIKKFAELDFKIKSGQIESDTGLEFLLFSL"
     gene            complement(2583..2852)
                     /gene="rpsT"
                     /locus_tag="PLQ22_01305"
     CDS             complement(2583..2852)
                     /gene="rpsT"
                     /locus_tag="PLQ22_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005389224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH58630.1"
                     /translation="MANIKQQAKRNITNEKRRLRNVSLKSSVRTAIKKVELAISNDNY
                     AEALELYKVAAKRLDTAQGKGVYHKNAVARKKSHLCKAINKIAPK"
     gene            2931..4019
                     /locus_tag="PLQ22_01310"
     CDS             2931..4019
                     /locus_tag="PLQ22_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH58631.1"
                     /translation="MNKINIYGAGISGMTAAIYLKKRNPKLDITLYEANDRVGQKILK
                     TGNGKCNLSNTDLDNASNYNNPSFMEEVLKITNSKDLKAFILDDLGLVLREDDYGRLY
                     PYSEKATSVLTAYLQKLKELDIKVVTSFRGDLDLKGITIIATGSYAQSKYNGYNYLTN
                     LGHKITDLNPALTTLKVKEDLRPLKGIRFKVKASILKGNKVIFSEEGEVQFREDALSG
                     IVTLDLSRYYKEGCEVSFDLVPEYSKEELEMLKAKFNDEDQFLNSLLSKMLALEVKKR
                     GNIISSLKDFKFTILGLNGYNEAQITKGGLDLSEIGYDFSSKIRPNLYVIGEVLDIDA
                     RCGGFNIGFAIISGITAGKSILKKHNSL"
     gene            4064..4666
                     /gene="pgsA"
                     /locus_tag="PLQ22_01315"
     CDS             4064..4666
                     /gene="pgsA"
                     /locus_tag="PLQ22_01315"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOH58632.1"
                     /translation="MNLPNKLTLFRVALIPVIVIIALISPLYDIYIFKEVTVGNFIIL
                     IVFLIAAFTDYLDGHIARKNNLITDFGKFMDPMADKVLVISTLLILLEQGKMTAFGFP
                     LGFVLILIVAREFMVTGIRLIAVQNNVVIAASKLGKIKTVSQMIMVIFLLLECYPFTF
                     IGGNAKDITALILIGFAGLTTLVSGIDYFIKNKDVILKSK"
     gene            4677..5660
                     /gene="recA"
                     /locus_tag="PLQ22_01320"
     CDS             4677..5660
                     /gene="recA"
                     /locus_tag="PLQ22_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOH58633.1"
                     /translation="MAKEKEQALKEAFKQIEKEYGKGSIMKLGDHPIGDIEVISSGSL
                     KLDIALGVMGYPRGRIVEIYGPEGSGKTTFALHAIAEAQKAGGYAAFIDAEHALDPKY
                     AAALGVDIENLILSQPDTGEQALEIAEYLIRSGAIDIIVIDSVAALVPKAEIDGDMGD
                     AHVALQARLMSQAMRKLSGVISKTKTIAIFINQIREKVGVFFGNPETTTGGRALKFYS
                     TIRLDVRRIEQLKSEGDPIGNLTRVRVVKNKVAPPFRVCDVDLIYGKGVSRTAEIIDM
                     AVDKDIVKKSGSWYSYNDEKLGQGKENVKKFLEENPNLTKEIEDKVREAYK"
     gene            5813..7399
                     /gene="rny"
                     /locus_tag="PLQ22_01325"
     CDS             5813..7399
                     /gene="rny"
                     /locus_tag="PLQ22_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOH58634.1"
                     /translation="MNKLIVMAVSPAVTVVLIVLGSILLLAGGFALGFFIVRSKLKKA
                     GQSAKAFLEVAQKEADEYKKELILGAKQEVHLLKQEFEKENRERTNYLNSLETKVTQR
                     EERLDNRSINLDKRESTISRKEEALEKQKVELEEKTSKVETLVQEQTEKLLEISKLDK
                     EEAKKIVLKQVEEEMDLEISQIVKEGVDKANAEVHKKTKELLTQVIQTYAGEIVAEQT
                     VSVVNLPNDEMKGRIIGREGRNIRALEAATGVDLIIDDTPEAVVVSCFDPVRREVARR
                     TLEALISDGRIQPTRIEEIVQKFEKEMDLEIKEAGDKAVYDTGIGRVHPELVKLLGRL
                     KFRTSYGQNALAHSIEVAMIAGNLAVEIGENEIIARRAGLFHDIGKAVDTTMEGSHVE
                     LGADIVRRYRERPEVIDAVLSHHGDETANSIIAVLVATADTISAARPGARNESIEAYI
                     KRLETLEALVSKHKGVERSYALQAGREIRVMVKPAEINDAQAEKLALELKKEIEKELT
                     YPGTIKITVIRETRVNQVAK"
     gene            7470..8246
                     /locus_tag="PLQ22_01330"
     CDS             7470..8246
                     /locus_tag="PLQ22_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOH58635.1"
                     /translation="MKILIVGDIYSKLGRKAFASGLAKIKAKETINFLIVNGENITHG
                     KGMNEKHYKWLLEQGVNVVTLGNHAFNNKEIFNYINEAKNIVRPANMETEYGVGYVTK
                     KYNDLTITVIQVLGQIFMPGNNTKNPFLVVDEILENVKSDIYICDFHGEATSEAVAFG
                     YYFTGKIDIIFGTHTHVQTNDATTLNGKTAYITDVGMTGSLESVIGVQKDIIISRFLG
                     KETLKFEPENEGKTQFSAILVEYDEQLKKVSKIETIRIIE"
     gene            8255..8818
                     /locus_tag="PLQ22_01335"
     CDS             8255..8818
                     /locus_tag="PLQ22_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH58636.1"
                     /translation="MLPIIETERLILRLIDDEDIHDFYEFAKLPDVAHLAGWRPHVNE
                     KETKIVIRMSRDKIRFGQLGNFAIILKETGKMIGTIELHSYTKDYKAELGFCLNPKYW
                     NQGYITEAAKAVVVWGFDEVHLKRIEAHTFLDNLSSQKVLEKINFTFEGIKKKGYQAH
                     DGKIYDVKAYGITDDDFYNLVRKNGIK"
     gene            complement(8811..9851)
                     /locus_tag="PLQ22_01340"
     CDS             complement(8811..9851)
                     /locus_tag="PLQ22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58637.1"
                     /translation="MKKYLLYFALHYPKSEIEDYIKMIHQSEYHLNETITNDSYAFLV
                     QEYEKAKPNSKFLYEYISDDVVRVNLGNYKASEFSLHYLFDSFKETINNNQGSIASMD
                     EKLTLLLDLIKSKEIALDYDDSLKKIEDYKKAGYPVIHHSEAFKKAYDPHYRIIQSSN
                     LTPGLKDFHLFYFLDNLIKEKNKLIIAIDGRSSSGKTTVAQTLKEKYNATIIHIDHFF
                     LPNFLKTKERLAEIGGNINYEEIEKVLREIKHNTTYTYQAFDCKVQEYYPVTIETSNL
                     IVFEGVYSLHERLFPYYDASVFLTLSKKEQISRLKQRETPATYLQFLNEWIPLENEYF
                     TDNLYKKIGIII"
     regulatory      9986..10112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            10156..11517
                     /locus_tag="PLQ22_01345"
     CDS             10156..11517
                     /locus_tag="PLQ22_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH58638.1"
                     /translation="MDGVLFNILLKVAIVLIVGFIGGQVAKALKLPNVSGYLVFGVLL
                     GPSLGLIVPGFKGLINANDQDTLRFISEMALAFIAFSIGSEFNVANIKKQGKEITILT
                     AFEVIGAVVLVFLLMLVVPKPQSFNEAHGIIGYEPFAKPNIAMSMVLASMSAATAPAA
                     TLMVIRQYRAYGPVTRAILPITALDDIFGIIVFGFFSSIAQILVPSPGVDMPLWLMIS
                     KPFIEVFGSMIVGAVIGIVLSYAARKFDRERDNIQVLAVASIVLTVGLFNLLNHDKLL
                     GQYGISLSSLLSSIVTGAMIANLTRRPDRTFQSVNDFTTPFYIIFFTMAGASLDLGIL
                     KVEWMIAIVALVYIFARGFGKYIGTYIGARIARSNPNVRKWLGLGLLPQGGISIGLLV
                     IVGATLPSMYPIISTIIMLSILVYETTGPIFAKISISKAGEINGLDKLQELSSVEDLV
                     PQE"
     gene            11529..11888
                     /locus_tag="PLQ22_01350"
     CDS             11529..11888
                     /locus_tag="PLQ22_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58639.1"
                     /translation="MNVLFIVLNDLAYLDEILEEFVSLKVRGATIIESEGMAAAILKR
                     DGLGGLLGGGLFKVETKDIQQNSKTIFTVIPDEETLEKAVNSVRKIVERSRQKTVGFM
                     FTVPVSGIYPLSPKKPK"
     gene            12036..>12709
                     /locus_tag="PLQ22_01355"
     CDS             12036..>12709
                     /locus_tag="PLQ22_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH58640.1"
                     /translation="MKKQQEKYIEPSLSCARKRTKDEVEYSEFAFNPKVFGLGKGKTY
                     LLKTFGCQANLSDSEKIRGILNHLGFTETVEEKEADLILLNTCAVRKTAEDRSFGEIG
                     RIKSLKKTNHNLIIGMCGCMPQEEATINTLKTKHPEIDLVFGTHNINLLPEYLERILI
                     NKEKVIEVFSIEGNVIEGMPKVRDHKIKAWVDIMYGCDEFCTYCIVPYTRGKERSRKK
                     EDILNE"
BASE COUNT         4598 a         1528 c         2277 g         4306 t
ORIGIN      
        1 tattgataag tttttttctg aaccaggaaa tatgattatt aaaggtttag ttattggtga
       61 tagtaaaagt tttaccttag atttcgcctc agacttaaga ataaacggga cttctcattt
      121 gtttgcaatt tctggtgctc acgtaaatat cattacttat tttttaatta aagtttttgc
      181 taggtcaaaa tatcaagata aaatagtttc ttcactttta ataatttatc tgtttctaac
      241 aaatttcttg ccttcagtat tgagggcagt aattatgtat ttacttcata tcttaaataa
      301 aaagtatcag ttgcatttca aaactttaga tttactagca atcgctttta ttttaatgat
      361 tatcattaat cctttttatt tttttaattt aagtttccag ttaagtttta ttgtaacggc
      421 tattattatt ttgcttgctt ctttgattaa agataaaagt tctttaatcc aaacagtcat
      481 tatttcaact gcagctgtta ttgcaacttt accaataatt gcgaatatta attcagaagt
      541 aaatttacta tcacctttag taaatgttgt ttttattttc ttggttttag aaattttact
      601 tccactttct tttattatct tagttttacc ttttttaagt aaattttata atttattagt
      661 tcaaggattt atatttttaa acaacctttt tgctagtcaa ataaaaataa gtgtacctct
      721 accctatttt aaaatatatc attccttaat ttattatagt ttttttattg tgttttttag
      781 tttaaataaa tttaaatcaa gaaaaattgt tttatctttc ttccttattc tgttttcttt
      841 tttcattttg tttcgttcat atactactag tttaaaagtg acatttttag atctttacaa
      901 tggcgaatca attttgattc aaaaaggagt aagtaaagtt ttgattgata ctggagcagg
      961 gcaaggaaag aaagtaagta gttttttaag aaaatctgga atttatcatt tagattattt
     1021 aattattact catgatcatt tcgaccatta tggggaagca aaggacattt gtgatgtggt
     1081 taatgtgaaa agggttataa cctcatcaat atctaagtta tcttttcaaa ggcaaatcaa
     1141 aatcggctct ccttatatca taaaattagg aaaagatgaa ttaatcttat atcccccaga
     1201 aaaacagtat ttagatgaga ataataatag tattattact tttctaaaaa gcgaattaaa
     1261 atttttattt ctaggtgatt tagttatgac tggcgaagaa agattgaatg gttttaaaga
     1321 aaaaatagat gttttaaaag tgggacatca tggttctcaa acagcaacat cgcctaatct
     1381 tctaagtatt ttaaaaccaa aatatgctat tattcaaact ggtagagtta aatcatttgg
     1441 cttccccgat catgagttaa ttaatcgatt aaataattat caaataaaaa catatcgaac
     1501 tgatcttcat tacacaattg tttatcaaaa taaaaaattt acttatctaa atcaatagca
     1561 gtattcttta aaatatggta aaatataaac aagaggtaca atatggctaa agtatattta
     1621 tttttgggaa atgaggaata tctaaacaaa gtcaaaattg aaaggattat taaagaatct
     1681 gtagctgacg aatacaatat caactattac gatatggaag aaaaaaacgt tagtttcgca
     1741 gttgaagatg cgcaaactgc tccttttttg tgtgaagaaa agattgttgt tttaagacat
     1801 ccaaagtttc taacaacagc caagttagag attgaacatg atattaaagg atttgtcaag
     1861 tatttaaata atcctagtcc gtacactatc tttattattg atgctagtaa tttaaaacta
     1921 gacaatcgta aagaagtagt aaaagtctta cttaaagttg cgattaaaga agaaagcgaa
     1981 tcattgtctg atgttgagtt tgtaggttgg gtaattagac aattttctca aaacaattta
     2041 aaaattggtc aaagagctgc gcaaactttt ttaagtagaa cgggaacaaa tttaataaat
     2101 gcaagtaatg aaatagataa gttactttta tattgtatgg ataaagatga agttacagaa
     2161 gaagatgtaa ttaacttaac tacaaaagaa ttagaacagg atatttttag ttttactaat
     2221 gcaattatgg aaaaaaataa aatgaaggca attgaggttt atcgtgatct tgttgctaca
     2281 aacaaatttg aacctgtaca attattaatt acttcaagta ataatttgaa agattgttat
     2341 ttagtaaaac tattgatgga gaaaaattac aagcaaaatc aaatcgcaga aagtttagga
     2401 gtttctcctg gtagagcata ctatctaatg cgtgatgctc gttctttaga taaagaaaga
     2461 gcagctgaac ttattaaaaa gtttgctgaa ttagatttta aaataaaatc tggccaaata
     2521 gagtcagata caggtttaga gtttttatta tttagcctat aaaaaaagcc gttattcacg
     2581 acttattttg gcgctatttt attgatagcc ttacaaagat gagatttttt tctagcaact
     2641 gcgtttttat ggtatacgcc tttaccttga gctgtgtcaa gtcttttagc tgctactttg
     2701 taaagttcta aagcttctgc gtagttatca ttagaaatag ctaattctac ttttttaata
     2761 gctgtacgta ctgatgattt taaagaaaca tttcttagtc ttcttttttc atttgtaata
     2821 ttacgtttag cttgttgttt aatatttgcc atagtaacct ccaatattta tctatagtat
     2881 tataccaaat aatttataaa aaagcaagga aataaaatcc gaggtggaaa atgaataaaa
     2941 taaatatcta tggtgctgga atttcaggta tgacagcggc catttattta aagaaaagaa
     3001 atcccaaatt agatattact ttatatgaag ctaatgaccg tgttggccaa aagattctaa
     3061 aaacaggaaa tggtaagtgt aatttaagta atactgattt agataatgct agtaactata
     3121 ataatccttc atttatggaa gaggtgctaa aaataactaa tagcaaagat ttaaaagctt
     3181 ttattttaga tgatttagga ttagttttaa gagaagatga ctatggaaga ttatatccat
     3241 atagtgaaaa agcaacatca gttttaactg cttacttaca aaaacttaaa gaattagata
     3301 tcaaagttgt aacgtctttt cgtggtgatt tagatttaaa aggaattaca atcatcgcaa
     3361 ctggaagtta tgctcaaagt aaatataatg gttataacta tttaactaat ttaggacata
     3421 aaataactga tttaaatcct gctctaacaa ccctaaaagt aaaagaagac ttaagacctt
     3481 tgaaaggcat tcgctttaaa gtaaaggcta gtattttaaa aggcaataaa gttatattta
     3541 gtgaagaagg tgaagtgcaa tttagagaag atgccttatc aggaattgtt actttagatc
     3601 tttcgcgtta ttataaagaa ggatgtgagg taagttttga tttagttcca gaatattcaa
     3661 aagaagagtt agaaatgcta aaagcaaagt ttaatgatga agatcaattt ttaaattcac
     3721 ttttatcaaa aatgttagct ttagaagtta aaaaaagagg taatattata tctagtttaa
     3781 aagattttaa atttactatt ttaggtttaa atggttataa tgaagctcaa attactaaag
     3841 gcggtttaga cttaagtgaa attggttatg atttttcttc taaaattaga cctaacttat
     3901 atgtaattgg agaagtgtta gatattgatg cgaggtgtgg aggattcaat attggctttg
     3961 cgattatctc tggtataacg gccggaaaaa gcattttaaa aaaacataat tctttataaa
     4021 ttgagttatt tattgtataa tataatatat agaaggtgat tatatgaatc taccaaataa
     4081 attaacattg tttagagttg cattaattcc agttattgtc atcatagctt taattagtcc
     4141 tctttatgat atttatattt ttaaagaagt tacagtagga aactttatta ttttaattgt
     4201 ttttttaata gctgcgttta cagattattt agatggacat attgctagaa aaaataatct
     4261 tattacggat tttgggaaat ttatggatcc aatggctgac aaagtcttag taatctctac
     4321 attgcttatc cttttagaac aaggtaaaat gacagcattt ggttttccgt taggatttgt
     4381 tttgattctt attgtagcta gagaatttat ggttacagga attaggttaa ttgctgttca
     4441 aaataatgtt gtaatagctg caagtaaact aggcaaaata aaaacagtat cgcaaatgat
     4501 tatggtaata tttttattac ttgaatgtta tccatttact tttattgggg gaaatgcgaa
     4561 agacataact gcgttaatat taattggctt tgcagggcta actacgctag tatcgggaat
     4621 agattacttt attaaaaata aagatgtaat attaaaatca aaataggagg aagaaaatgg
     4681 caaaagaaaa agaacaagct ttgaaggaag ccttcaagca aatagaaaaa gaatatggta
     4741 aaggttctat tatgaaactt ggtgatcacc caattggtga tatcgaagtt atttcatctg
     4801 gatctttaaa attagatatc gcacttgggg ttatgggtta tccaagaggt agaattgtag
     4861 agatatatgg gcctgaaggt tcaggtaaaa ctactttcgc tttacatgcg attgccgagg
     4921 cacaaaaggc tggagggtat gctgctttta ttgatgctga gcatgcttta gaccctaaat
     4981 atgcagcagc tttaggagta gatattgaga acttaattct ttctcaacca gatactggag
     5041 aacaagcttt agagattgcg gaatatttaa ttagaagtgg tgcgattgat attattgtaa
     5101 ttgattcagt tgcagcttta gtgccaaaag cagaaattga tggtgatatg ggagatgcac
     5161 atgtagcatt acaagcaaga cttatgagtc aagcgatgag aaaattaagt ggtgttattt
     5221 ctaaaacaaa aacaattgct atctttatta accaaattag agaaaaagta ggagttttct
     5281 ttggtaaccc agaaactaca actggtggta gagctttaaa gttctactct acaattcgtt
     5341 tagatgttag acgtattgaa caattaaaga gtgaaggaga tccaataggt aatttaacta
     5401 gagttagagt tgtaaagaat aaagtagctc ctccatttag agtttgtgac gttgatttaa
     5461 tttatggtaa gggtgtttct cgtacagctg aaattattga tatggcagtt gataaagata
     5521 ttgtcaagaa atcaggatct tggtattcat acaatgacga aaaattaggt caaggaaaag
     5581 aaaacgtgaa gaagttctta gaagaaaatc ctaaccttac taaggaaata gaagataaag
     5641 ttagagaagc atataaataa tagtcttgtt tatcaagact atttttttat aataggtaaa
     5701 aaaaggctta aatcttttaa ttgactaaac cacttatttg gtgtataata attatagata
     5761 tttatataaa gtaatttata aagatcagat atattaaatg atggaggtag atatgaacaa
     5821 attaattgtt atggcggtat cacctgctgt aacagtagta ttaattgttc taggctccat
     5881 attgctatta gctggcggat ttgctcttgg ctttttcata gttcgctcca aactaaaaaa
     5941 agctggacaa tctgctaaag catttttaga agttgctcag aaagaagctg acgaatataa
     6001 aaaggaattg atacttggtg ctaaacaaga agttcattta ttaaaacaag agtttgaaaa
     6061 agaaaataga gaacgtacaa attatttaaa ttcattagaa actaaagtaa cgcaaaggga
     6121 agaaagatta gataatcgta gcatcaatct tgataaaaga gaaagtacta tttctcgtaa
     6181 agaagaagct ttagaaaagc aaaaagtaga attagaagaa aaaactagca aagtggagac
     6241 actagttcaa gaacaaacag agaaattatt ggaaatttct aaactagaca aagaagaagc
     6301 aaagaagata gttctaaaac aagttgaaga agaaatggat ttagaaatat cacagattgt
     6361 gaaagaggga gttgacaaag ctaacgccga agttcataag aagacgaagg aacttttaac
     6421 acaagttatt caaacttatg caggtgagat tgtagctgaa caaacagttt cagtagttaa
     6481 cttacctaat gacgaaatga agggaagaat aataggaaga gaaggaagaa acattcgtgc
     6541 tttagaagct gcaactggag ttgatttgat tattgatgat acaccagaag ctgtagttgt
     6601 ttcttgtttt gatcctgtta gaagagaagt tgctagaaga actttagaag ctttaatttc
     6661 tgatggtaga attcaaccaa caagaattga agaaattgtt caaaagtttg aaaaagaaat
     6721 ggatcttgaa attaaagaag ctggagataa agcagtttat gatacaggta ttggccgtgt
     6781 acatccagaa ttggtaaaat tacttggtag gttaaaattc cgtactagtt atggccaaaa
     6841 tgctttagct cattcaatcg aagttgcaat gatcgcaggt aatctagcag ttgaaatcgg
     6901 ggaaaacgaa attatagctc gtcgtgcagg cttattccat gatattggaa aagcagtaga
     6961 tactactatg gaaggtagtc atgtagagtt aggtgctgat attgtaagaa gatatcgtga
     7021 aagaccagaa gtcattgatg cagtattaag ccatcatgga gatgaaacag ctaattcaat
     7081 tattgctgtt ttagtggcaa cagctgatac tatttctgct gctcgtccag gagcaagaaa
     7141 cgagtctatc gaagcatata tcaaacgttt agaaacccta gaagccctag tttcaaaaca
     7201 caaaggtgta gagcgtagct atgcattaca agcaggcaga gaaatcagag taatggttaa
     7261 accagctgaa atcaatgatg ctcaagcaga aaaattagct cttgaattaa agaaagaaat
     7321 tgaaaaagaa cttacttacc caggcacaat taagattaca gtaattagag aaacaagggt
     7381 aaatcaagtt gcaaaatagg aaatgagcaa tcatttcctt tttttataaa aatgtggtat
     7441 aatgtttata ttctagaagg cggtactaaa tgaaaatatt gattgtaggg gatatttact
     7501 ctaaactagg aagaaaagct tttgcttcgg gattagcaaa aattaaagca aaagagacaa
     7561 ttaactttct tatagtaaat ggggaaaata taactcacgg aaaaggaatg aacgagaaac
     7621 attataaatg gttactagag caaggagtca atgtagttac tttaggtaat catgctttta
     7681 ataataaaga gatctttaac tatataaatg aggcaaaaaa tattgtaaga cctgcaaata
     7741 tggaaactga atatggtgtt ggatatgtta ctaaaaaata caatgaccta acaattactg
     7801 taattcaagt tttaggccaa atttttatgc ctggtaataa tactaaaaat ccttttttag
     7861 ttgtagatga aattttagaa aatgtaaaaa gcgatattta tatttgtgat tttcatggtg
     7921 aagctacaag tgaagctgtt gcttttggtt attactttac aggtaaaata gatattattt
     7981 ttggaactca tacgcatgtt caaacaaatg atgctacaac tctaaatggg aaaacagctt
     8041 atattactga tgtaggtatg actggatctt tagagagtgt aattggggtt caaaaagata
     8101 tcatcatttc tcgtttctta gggaaagaga ctttaaaatt tgaaccagaa aatgaaggta
     8161 aaactcagtt ttcggcgatt ttagtagaat atgatgaaca attaaaaaag gtttctaaaa
     8221 tagaaacaat caggattatt gaataggagg atctatgtta ccaataattg aaacagaaag
     8281 attaatcctt cgtttaattg atgatgaaga catacatgat ttttatgaat ttgccaagtt
     8341 gccagatgtt gctcatttag ctggttggag accacacgtg aatgaaaaag aaacaaaaat
     8401 tgttattaga atgtctcgtg ataaaattag atttgggcag ttaggtaatt ttgccattat
     8461 tttaaaagaa acaggaaaaa tgataggcac aattgaactt catagttata ccaaagatta
     8521 taaagctgag ttaggatttt gtcttaatcc aaagtattgg aatcaaggtt atataacaga
     8581 agcagctaag gcagtagttg tttgggggtt tgatgaagta catttaaaaa gaatagaggc
     8641 tcatactttt ttagacaatc tatcatcaca aaaagtcttg gaaaaaatta attttacttt
     8701 tgaagggatt aagaaaaaag gatatcaagc tcatgatggt aaaatttatg atgtaaaggc
     8761 ctatggtatt acggatgatg acttttataa tttagtaaga aaaaacggga ttaaataata
     8821 atcccgattt ttttatataa attatctgta aaatattcat tttctaaagg aatccattca
     8881 tttaaaaatt gcaagtaagt agctggtgtt tctctttgct ttaaacgact aatttgttct
     8941 tttttactta gtgttagaaa gacgctagca tcataataag gaaatagtct ttcatgtaaa
     9001 ctataaacac cttcaaaaac aataagatta gaagtttcaa ttgttactgg atagtattct
     9061 tggactttgc aatcaaaagc ttgataagta taagtagtat tgtgtttaat ttctcttaga
     9121 actttttcta tttcttcata attaatatta ccacctattt ctgctaatct ttctttagtt
     9181 tttaaaaagt taggtaaaaa gaaatggtca atatgaataa tagttgcgtt gtatttttct
     9241 tttaatgttt gggctacagt agttttacca gagctagatc taccatcaat tgcaatgatt
     9301 agtttgtttt tttctttgat taaattatca agaaaataaa acaaatgaaa atcttttaat
     9361 cctggtgtta aattggaact ttgaataatg cgatagtgag gatcatatgc ttttttaaaa
     9421 gcttcactat ggtgaataac tgggtatcct gcttttttat aatcttctat cttctttaaa
     9481 gaatcatcat aatctaaagc tatttcttta gatttaatta aatctaaaag aagagttaat
     9541 ttctcgtcca tacttgcaat tgaaccttga ttgttattga tagtttcttt aaatgaatca
     9601 aataagtagt gaagtgaaaa ctcagaagcc ttatagttcc caagatttac tcttacaaca
     9661 tcatcgctta tatattcgta taaaaactta ctattaggtt ttgctttttc atattcttga
     9721 accaaaaaag cataactatc gtttgtaata gtttcattta ggtgatattc actttggtga
     9781 atcattttga tatagtcttc aatttctgat ttcggataat gaagagcaaa ataaagcaaa
     9841 tattttttca tacctcacct ctaacaagca tattctaaca caagattttc taatatacaa
     9901 tatacttgta tttaaatatt agtaaatgct aatttgcaaa tttaaatgta aacttcgttg
     9961 acaaaacaat aaaaatttga tagagtaata atgatctctg aatcattcga gtgggggacc
    10021 caggtcttgg ggtgaatcgc ttaagaaaag cgtaaggcaa cttaattgcc tgaatccgtc
    10081 agctaacctc ataggagtga ttaagcgtat aactatagtt atgcgttttt ttaatataaa
    10141 cggaggtaga ttaaaatgga cggagtgttg tttaacattt tattgaaagt cgcaatcgtt
    10201 ttgattgttg gctttattgg cggacaagta gctaaagctt taaaattgcc taatgtttct
    10261 ggctatttag tatttggtgt attattaggg ccatcattag ggttaattgt tcctggtttt
    10321 aaaggattaa ttaatgctaa tgatcaagat actttgagat ttattagcga aatggcttta
    10381 gcgtttattg cttttagtat tggtagtgaa tttaatgttg ctaatattaa aaagcaaggt
    10441 aaggagatta caattcttac agcatttgaa gtaataggag cagtagtatt agtattctta
    10501 ttaatgctag ttgtaccaaa acctcagagt tttaatgaag cacatggaat tattggttat
    10561 gagcctttcg caaaaccaaa tatcgcaatg tcaatggtac ttgcaagtat gtcagcagca
    10621 acagctccag cagcaacgtt gatggttatc aggcaatatc gtgcttatgg tccagtcaca
    10681 agagcaattt taccaattac tgctttagat gatatttttg gtattattgt atttggattc
    10741 tttagttcaa ttgctcaaat tttagtgcca tcaccaggag ttgatatgcc attatggtta
    10801 atgattagta aaccatttat agaagtattt ggttcaatga ttgtaggtgc tgttattggt
    10861 atagtactat cttatgcagc aaggaaattt gatcgcgaaa gagacaatat tcaagtatta
    10921 gccgttgcat caattgtgtt aacagttgga ttatttaatt tattaaatca tgataaatta
    10981 ttaggccaat acggaattag tctttcatca ttgttatcta gtattgtaac tggagcgatg
    11041 atagctaatc ttacaagaag accagaccgt acattccaat cagttaatga ttttactaca
    11101 ccattctata ttattttctt tacaatggct ggtgctagtt tagatttagg aattttaaaa
    11161 gtggaatgga tgatagcaat tgtagcattg gtatacattt ttgctcgtgg atttggtaaa
    11221 tacataggta cttatatagg agcaagaata gctaggagca atccgaatgt tcgaaagtgg
    11281 ctgggattag ggttattacc tcaaggaggg atctcaattg ggttgttagt aattgttggg
    11341 gcaacattgc catctatgta tccaattatt tccacaatca ttatgttaag cattttagta
    11401 tatgagacga caggaccaat ctttgcgaag atttcgatat ctaaagcagg agaaattaat
    11461 ggtcttgata aattacaaga attatcaagt gtggaagact tggttccaca agaataggag
    11521 aataaattat gaacgtatta tttattgtat taaacgattt agcgtattta gatgaaattt
    11581 tagaagagtt tgtatcatta aaagttagag gagctactat tattgaatca gaaggaatgg
    11641 cggcagctat tctaaaaaga gatggacttg gtggtttatt aggtggtggt ttatttaaag
    11701 ttgaaactaa agatatccaa caaaactcta aaactatttt tacagttata ccagatgaag
    11761 aaacactaga aaaagcagtt aattctgtaa gaaagattgt agaaagatca agacaaaaaa
    11821 cagtaggatt tatgtttact gttcctgttt caggaattta tcctttatcg cctaaaaaac
    11881 ctaaataacc tacctttctt aatgggcgat taaagaaata ttcaaaaaga aaaataagag
    11941 gcatgcctct tgttttttta tttggcctaa aaaatatact aaatctttac attacttggc
    12001 ttgttatgtt ataccctttg tgatataatc ttgatatgaa aaaacaacaa gagaaatata
    12061 ttgaaccatc tctaagctgt gctcgtaaga gaactaaaga tgaagttgaa tattccgaat
    12121 ttgcatttaa tccaaaagta tttggtttag gaaaagggaa aacctattta cttaagactt
    12181 ttggttgtca agcaaattta tctgatagtg aaaagataag aggaatttta aatcatttag
    12241 gttttacgga aacagtagag gaaaaagaag cagatttaat tttattaaat acttgtgcag
    12301 ttagaaaaac agcagaagac cgctcttttg gggaaatagg aagaattaaa agtttaaaga
    12361 aaacaaatca taatttaata attggtatgt gtggttgtat gcctcaagaa gaagcaacaa
    12421 ttaatacttt aaagacaaaa catccagaaa ttgatttagt gtttggaact cataatatta
    12481 atttgttacc tgaatatcta gaaagaattt tgattaataa ggagaaagtt attgaagttt
    12541 tttcgattga aggtaatgtt attgagggga tgccgaaggt aagagaccat aaaattaaag
    12601 cttgggttga tattatgtat ggatgcgatg aattttgtac atactgtatt gttccttaca
    12661 cgaggggaaa agaaagatca aggaaaaaag aagatatctt aaatgagat
//
LOCUS       DAOSGS010000015        12299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4332_length_12299_cov_5.244528, whole genome shotgun sequence.
ACCESSION   DAOSGS010000015 DAOSGS010000000
VERSION     DAOSGS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4332_length_12299_cov_5.244528"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3597
                     /locus_tag="PLQ22_01360"
     CDS             <1..3597
                     /locus_tag="PLQ22_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58641.1"
                     /translation="LNKKQREALYNASIGNSFVIVGRLGTGKTLTLTNICLEAITQGK
                     RILYISNQDETLETVFNTFNNLNLENYVVNFANSFAYFNYGEVSPYPKGKFEELTEKA
                     EVLELYKEVNDYESKIYSKIQDYRYVEVLTELAVLSLHKKKLLEIDNLDILYKYEFLD
                     VIKALEEIESYRKIIPSISKNKWHQVLISSDFLSRNNILDVLKQIKEDFLIIEKEKQT
                     LETKYGVVPQDSLSRFRNTVSNIKKLALADLPESWKKVGFVSYEEAIEEFEGLKAIIN
                     SLKEIEYSLDAKWKDYKQIDIDALIKEALGKHSKKDIEYINALLKARTDVTSLNNNFA
                     YQKELYEINSRILEEAFGIELYLNDEMQENLIEFLDLYKNGDFKFKDLVVYSQDKIFV
                     RQLFRAEAIALRYSRMIDEFQKEYPYIKMNNLESYVSILAKDKKGKMDKQILNAYRKE
                     GQRKVKEDSETLIKKTKQAIDSQRGLMKLDRIYENHFLGSDFLGTVRRYKNIETFFEK
                     NRNVNFKKALVKLAIALETYKEQEKTELTNALNAYSGSIGEINKLTRSYAVFGFESKK
                     NSFKDIYQELTNFYDYISSLYKTNDILLEQLVNRKKYVALEEYYILSESKKKISEYIK
                     GLENNKKYQKVYGVMYQNEETNLNTIQRTLQNFKKYCESFIDDSKVIASLNPKNYQEI
                     DEHLSTASEASEGLNRSFQNYAKYFQTGVSYFLYFDFSVIIEHLNTLLKKEKELDAYL
                     VINKNIRKINNYRLESLSDIIVSENKGNQLVDSFKYTYFTYLKNEFSKKEPNNEDCQE
                     ALNKIMVLEKTLIEKDINNLVVDIRKHSSSRFSTHNIKELDYRKYIKRTKGTKYLFLA
                     PVSLLNYYLDVNDFDLVIIDDAQLLNASEYYNAVLGKQIIISGELQPQGAVFNTLISR
                     MMNETIIKFDFRYLPTPKKLLNYLPGLQSLIKNTYSGNRGIEILKDKIVYYITLLYMT
                     NKDIKINIFIRNFEKQKEIYREIANSLISAHLSTNDIYTILTRRINIGDINNVYLYHA
                     DYNIIWLEDYYEVNLEHISLNILDTLLLCNERVIIYDSNNYIRSDNLFPKTLNGIVNE
                     DDAAIFEGVTYNQMNLELKKLIEAKGYKVYEAPGDINLIIEKNDRLYGVLLFFDYMKI
                     HYEILNEYRDYYEHYRASGFKMINVWITHFDNDLKKIADYIVKEIKNETKDK"
     gene            3578..4150
                     /locus_tag="PLQ22_01365"
     CDS             3578..4150
                     /locus_tag="PLQ22_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOH58642.1"
                     /translation="MKRKISKRTFNDFVDSLKDKTRSYYYINSNLNRLQKAGFNLSAK
                     LNNGDSLLHFAVKKDEVRLVKIFIKYGIILDLADLKGLTPLHLAVKDNKYQVAEALLE
                     AKANPNLGAELDQTPLHLAVINGNVEMIELLRKYGADCFLVDEKNQDSIDYAIDEKEL
                     PVIKHFYETVELDQGKRKKLEEIMEGVKNE"
     gene            4143..4946
                     /locus_tag="PLQ22_01370"
     CDS             4143..4946
                     /locus_tag="PLQ22_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOH58643.1"
                     /translation="MNEAWETLISNLKSRLENPLEVTLFVIDVVLAVIVYILVIGLIR
                     KKIKLSIIIPLSLGMIILHGLFVFFNMPIMTWILRVLSLLILFILVVIYSSEIRHYID
                     TKFLKRKVDNVFTSEEEKANVIKVLVDAMKQLSDRSIGALVTIEREDSLSNIIDNAIP
                     IDGKITQELLTTIFTPGTACHDGAVIIRKDRIVCAGAYLPTTERYDIPTFLGTRHRAA
                     IGASERYDAITIVVSEETGNISITSDGVITIDITLEKLQDLLDRYLLNK"
     gene            4955..5308
                     /gene="mgsA"
                     /locus_tag="PLQ22_01375"
     CDS             4955..5308
                     /gene="mgsA"
                     /locus_tag="PLQ22_01375"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOH58644.1"
                     /translation="MKIALIAHDKKKDELIDFCIQYEDFFKKHELVATGTTGRRIMEK
                     TNLKITCLNSGPLGGDIQIGNLVVSGEISIVIFFRDVLTAQPHEPDISALLRLCDLYN
                     IPLATNQRTAELLIQ"
     gene            5320..6879
                     /locus_tag="PLQ22_01380"
     CDS             5320..6879
                     /locus_tag="PLQ22_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58645.1"
                     /translation="MFNVIAQLVLQNPFVVFISLALLILALEITIIFMKKRFPVLTFL
                     LALLVIPIVLGAVVIGQFFPDLLTSAGITIDPIEIAFYYCIGLIAIQVIIFVIILLLK
                     LKDKRVIKEMKELHPEAQAIIKGKKIIFLDSYREKLPYKKKEIKSAKINDKKIGKKMF
                     YSYLRNKDAFLLYLELENKVLEVSFKTEKIIIDDKENAKALYVTIFKEGKQDDFVRSA
                     PFKIEDLLVYANLQKDVSMFYDQEIGSYRLSENMAHLLGTKEIVSEKSYQEIIIASDL
                     PIYLNREFSKEKPISYNYRVNSINGIDWVEQTTYYANGRYYDIVRLAKRPQAHYHYLG
                     TDSLVESLKKYEPKKKKVAIVLLTFNSLRKYLKQDPRLLESIIHEYFQKVLKEETIYQ
                     LSSLDYAYFIENKKAYDSLAKDLENKASKLLTDEVYVLENKYDIKNDVEVILSSSFKS
                     NDIFVQEVLEKLNLSEDLEVAVSVDNLNEVKLEPLVASPEAKEVVVPEEGYDFESSKI
                     DLNDDDLDEYK"
     gene            6866..7372
                     /locus_tag="PLQ22_01385"
     CDS             6866..7372
                     /locus_tag="PLQ22_01385"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOH58646.1"
                     /translation="MSTNKAKLRKKLKPIANKEEKDQKIVKYLEFFLEDKKVALYMAL
                     ADEPNLSKLLNEDIYLPKTNESISFYKNTGEFIKGRFGVLEPISKEKIEINDLDLIVI
                     PGLAYNKKGYRLGRGKGYYDRALENFSGFKIGLIYDDLLLDDEFQEDHDLKVDILITE
                     KRVIKFEV"
     gene            7375..8499
                     /gene="ispF"
                     /locus_tag="PLQ22_01390"
     CDS             7375..8499
                     /gene="ispF"
                     /locus_tag="PLQ22_01390"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOH58647.1"
                     /translation="MFDVIIVAAGSSTRFGENKILLDIHSKPLIIHTIEIFKDLEDLK
                     QIILVIKKEDQKEIEKILKEYDLDLVVTFGGKTRSESVVNGLKKVTSDYVLVHDGARC
                     ITLPSDVLKVLEEMKKHQAAFLMSFVSDSLYYEGKLIDRSKAYFSETPQGFKTEILKE
                     AFSLMKKEYPDEVSLVQDVLNIEAKKIKSNNFNIKITEPKDYTFVMERFVKRNLIGES
                     LDIHPFEFGKSLYIGGIKIPYHSGAKAHSDGDVLLHAISEAILGALGKGDLGSNFPDT
                     DPKYKDISSIYFLERVKEILEKEKTQIVNIDATIYLEAPQINSYLSKMEELIAHTLEI
                     DQDKVNIKATHFEGLGMIGEGKGLGSRAVVLLEKHDLRQR"
     gene            8538..8768
                     /locus_tag="PLQ22_01395"
     CDS             8538..8768
                     /locus_tag="PLQ22_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOH58648.1"
                     /translation="MDNIENKIKFILSKIRPYIRSHGGDVEYVKFEDGIVYVRMLGAC
                     VGCSALDTTLNDAIEAILLEEVPEVIGVENLP"
     gene            8779..9231
                     /locus_tag="PLQ22_01400"
     CDS             8779..9231
                     /locus_tag="PLQ22_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58649.1"
                     /translation="MNKYIIDKQDAKRKQLGITISRVISVLAAVVIVVAFFITFPSSA
                     DTEEAIEEARRQTLTYRYAFSFLVFSILSFSVIVFNEIFGGLYHLLKVIIKLLLAGLV
                     LVGGGLAIFLTDLHFTACQVSMIVSLAVLVYAFIPTIRGNLREELLKE"
     gene            9305..9754
                     /gene="tsaE"
                     /locus_tag="PLQ22_01405"
     CDS             9305..9754
                     /gene="tsaE"
                     /locus_tag="PLQ22_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH58650.1"
                     /translation="MKELLINNLEETKALGLKMGKLAFPNMMVVLEGDLGAGKTTFSK
                     AIALGLEIKEIVNSPTFVIMKTYKGRLPLYHIDAYRLSEASEDEYLEEYFEQGGVVII
                     EWGKNIEYLLPKEYLLISIKDLGLEKRKFSFKAYGKKYQKLLEEVLK"
     gene            9751..10326
                     /gene="tsaB"
                     /locus_tag="PLQ22_01410"
     CDS             9751..10326
                     /gene="tsaB"
                     /locus_tag="PLQ22_01410"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH58651.1"
                     /translation="MNALVMDTSTSFIFLYLIKDDKLVYKFEEEGENNHSEKLMLVIE
                     EALKKNDLSVEDLDLIVTGIGPGSYTGLRISLTIAKMFAWTKNIPLKVISSLVFLGSG
                     EPNGTYTVTNKAKKGYVYSLDIAINFDEIQILGKEKFLSVEENIIEAAAKKVSYIRGP
                     HYLINPFKIIKNAKEIESLHDLGPNYLREAI"
     gene            10327..10782
                     /gene="rimI"
                     /locus_tag="PLQ22_01415"
     CDS             10327..10782
                     /gene="rimI"
                     /locus_tag="PLQ22_01415"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOH58652.1"
                     /translation="MEKIRQMEIKDIIPVVRQEKAIFGESLGYDFFYQDLVLNPYQYY
                     YVLEKDEEIIGFIGAYINSDTCDVVSFYVIEEMQNHGYGKMLFNFLLDLCEEMNVNLL
                     TLEVRKSNKKAISIYERFGLNPIYIRRRYYRDGEDAILYGIQFERGKKK"
     gene            10779..11738
                     /gene="tsaD"
                     /locus_tag="PLQ22_01420"
     CDS             10779..11738
                     /gene="tsaD"
                     /locus_tag="PLQ22_01420"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH58653.1"
                     /translation="MIVLGVETSCDETAVSIVKDGKEVLANIVYSQIAIHQEFGGVIP
                     EVASRHHVLKITYVFEAALEKAKLKAKDIDLVAVTSNPGLIGSLMVGVNAAKAFALAN
                     GIEYIEVNHLIGHIYANYIERDFVFPLLALVVSGGHTELVLMRDHYQFEILGQTLDDA
                     VGEAYDKVGRVLDLPYPAGKPLDDLALTGVVTYDLPYVYLDKDSYNFSFSGLKSAVLN
                     LVNNTKQKGEEINKANLAASFQNSVVSVLVDKTIKAALEYKIKQIIVAGGVAANKGLR
                     FKLKEAVSKIPNLELTFPKFEYCTDNAAMIAVAGYFKYKLGIK"
     gene            11799..>12299
                     /locus_tag="PLQ22_01425"
     CDS             11799..>12299
                     /locus_tag="PLQ22_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH58654.1"
                     /translation="MQTRKRYLVLIAMFLLAVLVLVGCGERTYDYDKTGILSDGVFNY
                     KVVEGVKTVYREDEDGVPYQEEVDDSYVIIINTVERHKKLEIPETIDNLPVREIADYA
                     FFEYTKLFGLITVSRSKLEEVIFPNTLVKIGKFAFYQNTRLKSIYIPGSVKTISESAF
                     AGCYNVR"
BASE COUNT         4667 a         1266 c         2172 g         4194 t
ORIGIN      
        1 ttaaataaaa aacaaaggga agctttatat aatgcaagta taggcaatag ttttgttatt
       61 gtaggtaggt taggtactgg aaaaactcta accctaacaa atatttgttt agaagcaatt
      121 actcagggaa aaagaattct ttatatttct aaccaagatg aaactttaga aacagttttt
      181 aatacgttta ataatttgaa tttagaaaac tatgtagtta acttcgctaa ttcttttgct
      241 tattttaatt atggagaagt atcgccttat ccaaaaggga aatttgaaga gttaacagaa
      301 aaagctgaag tattagagtt atataaagaa gttaatgatt atgaatcaaa aatctattca
      361 aagattcaag actatcgtta tgtagaggta cttactgaac ttgcggtttt atcattacac
      421 aagaaaaaat tattagaaat agataattta gatattttat ataaatatga atttttagat
      481 gttataaagg ctttagaaga aattgaaagt tatcgtaaaa ttattccttc aatttctaaa
      541 aataaatggc atcaagtttt aatttcttca gactttttgt caagaaacaa tattcttgat
      601 gttttaaagc aaattaaaga agacttttta attattgaaa aagaaaaaca aactttagaa
      661 acaaagtatg gtgttgttcc tcaagatagt ttatcgagat ttcgtaatac tgttagtaat
      721 ataaagaaat tagccctagc tgatttacca gaatcttgga aaaaagttgg ctttgtaagt
      781 tatgaagaag caattgaaga gtttgaagga ttaaaagcaa ttattaattc tttaaaagaa
      841 attgaatata gtttagatgc taagtggaaa gattacaaac aaatagatat tgatgcttta
      901 attaaagaag ctttaggtaa acacagtaaa aaagatattg aatatataaa tgctttatta
      961 aaagcaagaa cagatgttac gtctttgaat aacaactttg cttaccaaaa agaactatat
     1021 gaaatcaatt caagaatttt agaagaagct tttggaattg agctttattt aaacgatgag
     1081 atgcaagaaa acttaatcga atttcttgat ctttataaaa atggagattt taaatttaaa
     1141 gatttagttg tttattctca agataagatt tttgtaagac agttatttag agcagaagca
     1201 atagctcttc gttattcaag aatgatagat gagtttcaaa aagaataccc atatataaag
     1261 atgaataact tagaaagtta tgtaagtatt ttagctaaag acaaaaaagg taagatggac
     1321 aaacaaattt tgaatgctta tcgtaaagaa ggtcaaagaa aagttaaaga agactctgag
     1381 actttaatta aaaaaacaaa acaagctatt gatagccaac gtggtttaat gaaactagat
     1441 aggatttatg aaaatcactt tttaggatct gattttcttg gtacagttag acgttataaa
     1501 aatattgaaa catttttcga gaaaaataga aatgtaaact ttaaaaaggc tttagtcaag
     1561 ttagctattg ctttagaaac atataaagag caagaaaaga cagaactaac taatgcttta
     1621 aatgcttata gtggttcaat aggagaaatt aataaattaa caagaagtta cgctgttttt
     1681 ggctttgagt caaaaaagaa tagttttaaa gatatttatc aagagttgac aaacttttat
     1741 gattatatta gtagtttata taaaacaaat gatatcttat tagagcaact tgttaatagg
     1801 aaaaagtatg ttgctttaga agaatactat attctttctg aaagtaaaaa aaagatttca
     1861 gaatatatta aaggattaga aaataataag aaatatcaaa aagtttatgg tgtaatgtat
     1921 caaaatgagg aaacaaattt aaatactatc caaagaacat tacaaaactt taaaaaatat
     1981 tgtgaaagct ttattgacga tagtaaagtt attgctagtt taaatccgaa aaattatcaa
     2041 gaaattgatg aacatttaag tactgcctct gaagcaagtg agggtttaaa tcgttcattc
     2101 caaaattatg ctaagtattt tcaaactggg gtttcgtatt tcttgtattt tgatttttca
     2161 gttattattg aacacttaaa tactttactt aaaaaagaaa aagaattaga tgcttattta
     2221 gtaatcaata aaaacattag aaagattaat aattatagac ttgagtcatt aagcgatatt
     2281 attgtttctg aaaataaagg taatcaacta gttgatagtt tcaaatatac ttactttaca
     2341 tatctaaaga atgaattttc taaaaaagag ccaaataatg aagactgtca agaagcatta
     2401 aataagatta tggttttaga aaaaacacta attgagaaag atattaataa tttagttgta
     2461 gatattagga aacacagcag tagtagattt agtactcata atattaaaga gttagattat
     2521 cgcaagtata ttaaaagaac aaaaggaact aaatatttat ttttagcacc cgtttcttta
     2581 ttgaattatt acttagatgt aaatgacttt gatttggtaa ttattgatga tgcccaatta
     2641 ctaaatgcga gtgagtatta taatgccgtt ttaggaaagc aaattattat ttctggggaa
     2701 cttcaacctc aaggagctgt atttaatact ttaattagta ggatgatgaa tgagacgatt
     2761 attaaatttg attttcgtta tttacctaca ccaaagaaac tccttaacta tttacctggt
     2821 ttacaaagtt taattaaaaa cacgtatagt ggtaatagag gaattgaaat tttaaaagat
     2881 aaaattgttt attatattac tttattatat atgactaata aggatattaa gattaacatc
     2941 tttatccgta attttgagaa acaaaaagaa atttaccggg aaatagctaa tagtttaatt
     3001 agtgctcact taagcacaaa tgatatttat actattctta caagaagaat taatattggt
     3061 gatataaaca atgtttactt atatcacgca gattataata ttatttggct tgaagattat
     3121 tatgaagtaa acttagagca cattagttta aatatcttag atactttact tctttgtaat
     3181 gaaagagtta taatttatga tagtaataac tatattagaa gcgataattt attcccgaaa
     3241 actttaaatg gaattgtaaa tgaggatgat gccgctattt ttgaaggtgt tacttataac
     3301 caaatgaatt tagaacttaa aaagttgatt gaggcaaaag gttataaagt ttatgaggca
     3361 ccaggagata ttaacttgat tattgaaaag aatgatcgtt tatatggagt attgttattc
     3421 tttgattata tgaagataca ttatgagata ttaaatgaat atcgtgacta ctatgaacat
     3481 tatcgagcta gcggttttaa aatgatcaat gtttggatta ctcattttga taatgattta
     3541 aagaaaatag ctgattatat tgtaaaggaa atcaaaaatg aaacgaaaga taagtaaaag
     3601 aacttttaat gatttcgttg attccttaaa agataaaact cgcagttatt actatattaa
     3661 ttctaatctt aataggttac aaaaggctgg atttaattta agtgcgaaat taaataatgg
     3721 tgatagttta ttacacttcg cagttaaaaa agatgaagtt agattggtta aaatcttcat
     3781 aaaatatggt ataatattag acctagcaga tttaaaaggt ttaactcctt tacatttagc
     3841 ggtcaaggat aataaatatc aagttgctga ggctctttta gaagctaagg ctaatcccaa
     3901 tttaggagca gaattagatc aaactccttt gcatttagct gtaatcaacg gaaatgtaga
     3961 gatgatagaa ctattaagaa aatatggtgc tgattgtttc ttagttgatg aaaaaaatca
     4021 agattcaatt gattatgcaa ttgatgaaaa agaacttcca gttatcaagc atttttatga
     4081 aactgtggag ttagatcaag gaaaaagaaa aaaattagaa gaaataatgg aaggtgtaaa
     4141 aaatgaatga agcatgggaa accctaataa gtaatttaaa atcaagactg gagaatcctt
     4201 tagaggtaac tctctttgtt attgatgttg tactagcagt aatcgtttat attttagtaa
     4261 ttggtttaat tcgcaaaaag attaaattat cgattatcat tccgctatcg cttggaatga
     4321 ttatcttaca tggattattt gtgtttttta atatgccaat tatgacttgg attttaagag
     4381 tactttcact tttaatcttg tttattcttg tagttattta ttcatcagaa attaggcatt
     4441 atattgatac taaattcttg aaacggaaag tggataatgt ctttacttct gaagaagaaa
     4501 aagctaatgt tattaaagta ttagtagatg cgatgaaaca attgtcagac cgttcaattg
     4561 gtgctttagt tacaattgaa agagaagata gtttaagtaa tattattgat aatgcaattc
     4621 cgattgatgg taaaattacc caagagttac taacgactat ctttacgcca ggtactgctt
     4681 gtcatgatgg agccgtaatt ataagaaaag atagaattgt ttgcgctgga gcgtatttac
     4741 ctacaacaga aagatatgat atcccaacat ttttaggaac aagacataga gctgcaattg
     4801 gtgcttctga aagatacgat gcaattacaa ttgtcgtttc tgaagaaaca ggcaatatca
     4861 gcattacatc tgatggcgta attactattg acattacttt agaaaaatta caagatttat
     4921 tagatagata tttattaaac aaataggagg tcaaatgaaa atagcactaa ttgcccatga
     4981 taagaaaaaa gatgaattaa ttgatttttg tattcaatat gaagattttt ttaaaaaaca
     5041 tgaacttgta gctacaggta ctacaggtag aagaattatg gaaaaaacta atttaaagat
     5101 tacttgtttg aattctggtc cattaggtgg agatattcaa attggtaatt tagtagtaag
     5161 cggagaaata agtatcgtta ttttcttccg tgatgttttg acagctcaac cgcatgagcc
     5221 agacatttca gctcttttga gactttgtga tttatataac attcctttag cgactaatca
     5281 gaggacggct gaattattaa ttcaatagga ggataaaata tgtttaatgt tattgcacaa
     5341 ctagtgcttc aaaacccgtt cgtcgttttt atttctcttg ctttgttgat attagcatta
     5401 gaaattacga ttatctttat gaaaaaaagg tttccagttc taacattttt attagcgctt
     5461 ttggtaatcc ctatagtatt aggagcagtt gtaatcggcc agtttttccc agacttatta
     5521 acttctgctg gtataacaat tgatccaata gaaatagctt tttattactg tattggttta
     5581 atcgcaatcc aagtaataat ttttgtaatt attttattat taaaattaaa agataaacgc
     5641 gtaatcaaag aaatgaaaga attacatcct gaagcccaag cgattattaa aggaaagaaa
     5701 attatctttt tagatagtta tcgtgaaaaa ttaccgtata agaaaaagga aattaaatcg
     5761 gctaaaatta atgataaaaa aataggtaag aaaatgtttt atagttattt aagaaataaa
     5821 gatgctttct tgctttattt ggaattggaa aataaagttt tagaagtaag ttttaaaaca
     5881 gaaaaaatta ttattgatga taaagaaaat gctaaagcac tttacgtaac tatttttaaa
     5941 gaaggtaaac aagatgattt tgttaggtca gctcctttta aaatagagga cttgcttgtt
     6001 tatgcaaact tacaaaaaga tgtttcaatg ttttatgatc aagaaattgg tagttatcgc
     6061 ttaagtgaaa atatggctca tttattagga actaaagaga ttgttagtga aaagtcgtat
     6121 caagaaatta taattgctag cgatcttccg atttacttaa atagagaatt ttcaaaagag
     6181 aaacctatta gttataatta tcgtgttaat tcaattaatg gtattgactg ggttgaacaa
     6241 actacatatt atgctaatgg tagatattat gatattgtta ggctggctaa aaggccacaa
     6301 gcacattatc attacttggg cacagattct ttagttgaat cattaaagaa atatgagcca
     6361 aagaagaaaa aggtagccat agttttgctt acatttaatt ctttaagaaa atacttaaaa
     6421 caagatccaa gattacttga aagtattatt cacgagtatt tccaaaaggt tttgaaggaa
     6481 gagactattt atcaattatc tagtttagat tatgcttatt ttattgaaaa taagaaagca
     6541 tatgatagtt tagctaaaga tttagaaaat aaagcttcaa agttattgac agatgaagtt
     6601 tatgttttag aaaacaaata cgatattaag aatgatgtag aagttattct aagttctagt
     6661 tttaaatcaa acgatatttt tgttcaagag gttttggaaa aattaaattt aagtgaagat
     6721 ttagaagttg cagtatcagt agataatcta aatgaagtta aattagaacc tttagttgca
     6781 agtccagaag caaaagaagt tgttgttcca gaagaaggtt atgattttga atcttctaaa
     6841 atagatttaa atgatgatga tttagatgag tacaaataaa gctaaattaa gaaaaaagtt
     6901 aaaacctatt gcaaacaaag aagaaaaaga tcaaaaaata gttaagtatt tggaattctt
     6961 tttggaagat aaaaaagtag ctttgtatat ggcattagca gatgagccca atttaagtaa
     7021 attacttaat gaagatatct atttaccaaa aacaaatgaa agtattagtt tttataaaaa
     7081 cacgggtgaa tttattaaag gtaggtttgg agttttagaa ccaatatcta aagagaaaat
     7141 agagattaat gatttagact taattgttat tcctggatta gcttataata aaaaaggcta
     7201 tcgtttagga agaggtaaag gctactatga tcgtgcttta gaaaactttt ccggttttaa
     7261 aattggcctt atttatgatg atttattgct agatgatgag tttcaagaag accatgatct
     7321 aaaggtagat atcttaatta cagaaaaaag agttattaag tttgaggtgt aaatatgttt
     7381 gatgttatta ttgtagctgc tggtagtagt actagatttg gagagaacaa aatactttta
     7441 gatattcact ctaagccttt aattattcat acaattgaaa tattcaaaga tttagaagat
     7501 ttaaagcaaa ttattttagt aattaaaaaa gaagatcaaa aagaaattga aaaaatactt
     7561 aaagaatatg atttagattt agttgttact tttggtggta aaacaagatc tgaaagtgta
     7621 gttaatggtt tgaaaaaagt aactagtgat tatgttttag ttcatgatgg agctagatgt
     7681 attaccctac caagtgatgt tttgaaagtt ctagaagaga tgaaaaaaca tcaagcagct
     7741 tttctaatga gttttgtttc tgactcgtta tattatgaag gaaaattaat tgatagaagt
     7801 aaagcttact tttcagaaac acctcaagga tttaagacag aaatactgaa agaagcattt
     7861 tctttaatga aaaaagaata tccagatgag gtaagtttag ttcaagacgt tttaaatatc
     7921 gaagctaaga aaataaaatc aaataatttc aatattaaaa ttactgagcc aaaagattat
     7981 acttttgtta tggaaagatt tgttaagcgc aatttaattg gtgaaagttt agatattcat
     8041 ccatttgagt ttggtaagag tttatatata ggtggtatta aaatacctta tcatagtggg
     8101 gctaaagctc attctgatgg tgatgtttta ttgcatgcaa tttctgaggc tattttaggg
     8161 gctttaggaa aaggtgattt aggcagcaat ttccctgata ctgaccctaa atataaagat
     8221 attagttcaa tttatttttt agaaagagta aaagaaatct tagaaaaaga aaaaactcaa
     8281 atagtaaata ttgatgctac aatttattta gaggctccgc aaatcaattc ttatctaagc
     8341 aaaatggaag agttaattgc tcatacttta gaaattgacc aagataaggt taatattaag
     8401 gctacccatt ttgaaggatt aggcatgata ggcgaaggca aggggcttgg aagtagagca
     8461 gtagttttac ttgaaaagca tgatttaaga caaagataaa aagttgtagt ataataataa
     8521 acaagaggtg aagaacaatg gataatattg aaaacaagat aaaatttatt ttaagcaaaa
     8581 tcagacctta tattagatca catgggggag atgtggaata cgtaaagttt gaagatggaa
     8641 tagtttatgt aaggatgtta ggtgcatgtg ttggttgttc tgctttagat acaacgctaa
     8701 atgatgcaat tgaggctata cttttagaag aagttcctga agttattggc gttgagaatt
     8761 tgccataagg agatttaaat gaataaatac attattgata aacaagatgc caaaagaaag
     8821 caacttggga taacaatttc tagggttatc tcagttctag ctgctgttgt aattgtagtt
     8881 gcattcttta taacttttcc ttcatcagcc gatacagaag aggctattga agaagcaaga
     8941 agacaaacac taacttatcg ttatgctttt agtttcttag tgttttcgat tttatctttt
     9001 tcagttatag tatttaatga aatctttggt ggattatatc atttacttaa agtgattatt
     9061 aagttactat tagcaggttt agttttagtt ggtggtggtt tagcaatttt cttaacagat
     9121 ttgcatttta ctgcttgtca agtatcaatg attgttagtt tagctgtatt agtttatgct
     9181 ttcatcccaa caatccgtgg taacttaaga gaagaattat taaaagaata attaagataa
     9241 ataaaagcaa ctcaaagatt gagttgtttt tatatttttg gtataataaa taaaggtgtt
     9301 aaacatgaaa gagttattaa taaataattt agaagaaaca aaagctttag gcttaaagat
     9361 ggggaaactt gcttttccta atatgatggt agttttagaa ggcgatttag gtgccggaaa
     9421 gactactttt tccaaagcta tagctttagg tttagaaata aaagaaattg taaatagccc
     9481 aacatttgta attatgaaga cttataaggg aagactccct ttatatcata ttgatgctta
     9541 ccgtttaagt gaagcatcag aagatgaata tttagaagag tactttgaac aaggcggagt
     9601 agtaattatt gaatggggta aaaatataga atatttgtta ccaaaagaat atttgcttat
     9661 ttcgattaaa gatttaggct tagaaaaaag aaaatttagt tttaaagctt atggtaagaa
     9721 atatcaaaag ttgttagaag aggtattaaa atgaatgcat tagtaatgga tacatcaaca
     9781 tcttttatct ttttgtattt aattaaagat gacaagttag tttataaatt tgaagaagaa
     9841 ggcgaaaata accattcaga aaaactaatg ttagttattg aagaagcttt aaaaaagaat
     9901 gatttaagtg tagaagattt agatttaatt gtaactggta ttggtcctgg tagttatact
     9961 ggtctaagaa ttagtcttac aattgcgaag atgtttgctt ggactaaaaa tattccttta
    10021 aaggttattt ctagtttagt gtttttagga agtggagaac ctaatggtac ttatactgtt
    10081 acaaataaag ctaaaaaagg ttatgtttat agtttagata ttgcaatcaa ttttgatgaa
    10141 atacaaatat tgggtaaaga gaaatttctt agcgttgaag agaatataat agaagcagca
    10201 gcaaaaaagg taagttatat tcgtgggcca cactatttga ttaatccttt taagattatt
    10261 aaaaatgcta aggaaataga atcattacat gatttaggcc ctaattattt acgtgaggca
    10321 atttaagtgg aaaaaataag acaaatggaa atcaaagata ttattcctgt agttagacag
    10381 gaaaaagcta tctttggtga atcgttagga tatgactttt tctatcaaga tttagtatta
    10441 aatccttatc aatattatta tgttttagaa aaagatgaag agataattgg atttatcggt
    10501 gcttatatta attctgatac ttgtgatgta gtttcttttt atgtaattga agagatgcaa
    10561 aatcatgggt acggaaagat gctttttaat ttcttgcttg atttgtgtga agagatgaat
    10621 gtaaatttat tgactttgga agtaaggaag agtaataaaa aagcaatttc tatttatgaa
    10681 agatttggtt taaatccaat ttatataaga agaaggtatt atcgagatgg tgaagacgct
    10741 attctttatg gtatccaatt tgaaagagga aaaaagaaat gattgtatta ggtgtagaaa
    10801 caagttgtga tgaaactgca gttagtatag tgaaagatgg aaaagaagta ttagcgaata
    10861 tcgtttattc gcaaattgct attcaccaag aatttggtgg tgttattcct gaagttgcat
    10921 cacgacacca tgttttgaaa ataacttatg tttttgaagc tgccttagaa aaagcaaaat
    10981 taaaagctaa agatattgat ttagttgcag taactagtaa tcctggttta attggatcat
    11041 taatggttgg agttaatgca gctaaagcat tcgcattagc taatggaatt gaatatattg
    11101 aagtaaatca tttaattggt catatttatg caaactatat tgaaagagat tttgttttcc
    11161 ctttacttgc tttagtagtt agcggtggtc atactgaact tgttttgatg agagatcatt
    11221 atcagtttga gattttaggc caaacattag atgatgcagt tggtgaggct tatgataaag
    11281 ttggaagagt attagaccta ccttatcctg ctgggaaacc tttagacgat ttagctttaa
    11341 ctggtgtagt tacttatgat ttaccttatg tttatttaga taaggatagc tataatttta
    11401 gtttttctgg tttaaaaagt gcagttttga atttagtaaa taatacaaaa caaaagggtg
    11461 aagaaataaa taaagctaat ttagctgctt cttttcaaaa tagtgtagtt tcagttttag
    11521 tagataaaac aattaaagct gctttagaat ataaaataaa gcaaataatt gtagctggtg
    11581 gagtagctgc taataaaggc ttacgcttta aattaaaaga agcagtatct aaaatcccta
    11641 atttagagtt aactttccct aagtttgagt attgtacaga taatgcagcg atgattgcag
    11701 ttgcagggta tttcaaatac aagctaggga taaaataata aatattattg cttttataga
    11761 gaatttatag tataataata aagaaatagg aggcagttat gcaaactaga aaaagatatt
    11821 tagttttaat tgctatgttt ctattagcag ttttggtgtt agtaggatgt ggcgagagaa
    11881 catatgacta tgataaaaca ggaattttaa gtgatggtgt ttttaattat aaagttgtag
    11941 agggcgtaaa aactgtctat agggaagatg aagatggtgt cccttatcaa gaagaagttg
    12001 atgatagtta tgtgattatt attaacacag tagaaagaca taaaaaacta gaaataccag
    12061 agacaattga taacttgcct gttcgtgaaa ttgccgacta tgcttttttt gaatatacaa
    12121 aattatttgg tttaattact gtttctcgaa gcaaattaga agaagttatt tttcctaaca
    12181 ctttagtgaa gattggtaaa tttgcttttt accaaaacac aagattaaag agtatttata
    12241 ttccggggag tgttaaaaca atttccgaat cagcgtttgc aggctgttat aatgttaga
//
LOCUS       DAOSGS010000016        11926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4523_length_11926_cov_4.778873, whole genome shotgun sequence.
ACCESSION   DAOSGS010000016 DAOSGS010000000
VERSION     DAOSGS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_11926_cov_4.778873"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="PLQ22_01430"
     CDS             complement(<1..2052)
                     /locus_tag="PLQ22_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOH58655.1"
                     /translation="MKLKDLSLKQKVGQMLMFSFHGTSYNEQVDYLLNELELGGLILF
                     KRNITSLKQVSKLNGEISQDKKIRPFIALDQEGGPVQRIEKGITPLLSAMGFAANGND
                     PYELYKSAGKNLKHLGFSINFAPVADVNNNPYNPVINSRSYSDNPELVSRYVLRASQG
                     FKDAKLIATVKHFPGHGDTNVDSHLGLPVVDKSLEEIEKVELYPFKKAIENGANGIMM
                     SHIVYQCLDKDDPASLSYNIITKLLKEKLGFKGLVVTDSLTMKAIWDNYSIKEIVKKG
                     VLAGNDILCFCGKADLNEQKEIYNTFVSLVEEGEIPLSRVDEAVKKILEYKEIYLEKD
                     LAFENNLSVIKREEKSKLAEDFALESITLVKDKGLVPIKKQEKIFAIFPEIKLFSLVD
                     NKENDYFTLQDFLDCNEVIINQEFNEYKLIKDEIRKADKVIFCTYNVTKGDYQTKVWQ
                     VLDPNKTIVVSMRSPYDILHLENVESYICLYEATLLSLKSLVKLIYSGKFKGSLPIKL
                     EGGNMKIIRVKDYEEMSKKAAEIIADVVKKNPKANLGLATGSSPLGTYKNLIKKYKAK
                     EISFKDIKSFNLDEYCQLDKNHEQSYYSYMNENLFKHIDIKKANTHLPSSEGDDLEAN
                     CKKYNDLLKKNPVDLQLLGIGGNGHIGFNEPGTAFTQETFVVKLAEKTREDNKRFFAS
                     ID"
     gene            complement(2106..3140)
                     /locus_tag="PLQ22_01435"
     CDS             complement(2106..3140)
                     /locus_tag="PLQ22_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH58656.1"
                     /translation="MTSKQKIVVPQEYYSKPVLTEHEKKTRRKVYAQKVGGRAGRIFR
                     TLILIGLCFVILLPIFQKFSYAFRSPNDLTNPQVVWIPSEFSTANIGLAMDLLDYWPT
                     LVRTTLLSLAIMFIQVMASAIAGYAFAKLRFKGSNILFYIILFTLVVPNETLHVSRSL
                     FLVNTTFFGIKLMGTVLSIFILSAFGQGIRSAIFIYLFRQFFRNIPAELEESAQVDGA
                     GVIRTFWSVMLPNARGAIVVVALFAFVWQWNDYYFARLFSIVQSYPVLAARLGEGTDR
                     LFTVISGWVAKGKPIFDTLTDEALATDPMFYGLIANTAALLMMLPLLIGYFFVQKQFV
                     EGIERTGITG"
     gene            complement(3130..4365)
                     /locus_tag="PLQ22_01440"
     CDS             complement(3130..4365)
                     /locus_tag="PLQ22_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH58657.1"
                     /translation="MNQNQAKKTFKEKWNDYFARRRYYIALEEQAKQDKIQAKREKEM
                     QKQLQKDQKAQAKRDKVTWYKKAKWAIQDFFHKISISKPVVKVKEALSFVPRKLTRNL
                     THRKKKALWGVVFVIPLIIGFGYFFLIPFITTVFYSFSHIQTIGLNPVTGDNMGVITE
                     NIGFDNYQFVWNQHSTFRQRLLTSFGNTLLEVPLVLIFSLIIAVVLNTQFPGRSFVRA
                     VFFMPVIFNSQAVEVAMKTGAALTAATAETTSTMFQGGFDFSFFLLEAGVPGGVVTFL
                     GNVTSSIYDVITYSGVQILIFLSAIQSVPKHLYEAAKMEGATQYEIFWKITFPMVSPL
                     MLTAGVYTVVDSFLRSDLLSILDYYSVNNFVALTGGLGMVTAHGIHAAMSWLFCLSSI
                     ILISLVLIVISRVVFYYDE"
     gene            complement(4368..8321)
                     /locus_tag="PLQ22_01445"
     CDS             complement(4368..8321)
                     /locus_tag="PLQ22_01445"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOH58658.1"
                     /translation="MKIIRLRHIVLGLLVIAISITLGVYFIGGLKATTISYDNEEFNS
                     EEFLLDFSHLEQYDRKITFKAGFPASDIDNVANALKTAGYKVEKTDYYGNTAIYLNLN
                     VGYNEEDIANEMYIAQLAIARLFEAGDFDMDIYEDNLGEPGVDNLKIVATTDKGKAAF
                     QEIVDKLTALNFFDTYETAIIQVPKEGGEEGEMEDQEIVLPNNTNIDFDNHTIVFDYP
                     IKLIETKMSELAIVQKSIPDPDEEVLANIRQDIQRAVGILNDIAESIVLNSVLDNIEY
                     KTSIFEEERIVAQNEKFTMYINEKTTAISIGLNSTGTPKKVGGLQVQDENGFLVYESF
                     NVLYQLSDYTNERGELVSGDVFRLRYFGRSGKVSATALGTYSKSVAYMDLIKGMATKH
                     YKINYAGDNKVQVLYEVGEFTNANNFFPKYIERAVFEDIVRGNTWITKVVSSERDSQG
                     HFNLIYRENGVTTNEEAANYIEANGLGKAKPAYSADGTNTFLGYWDLSGLLWTEADYE
                     DGKVTIDKVGKPKLVVGVNCNTKDSPIRVNPFLTAANIKSIMDGNYELIYNITTNETN
                     KNYIHYTANSSKTYENKATSEPRRNELYMFLYDISPTNYMRTSTSVIDKYDDSKIVYY
                     EGRPVVQGGVPLRDAEGNYVYDQLGNPVRAQFSEPFLAAEGEERRNIVEEQNRIYQQS
                     AASASAIFQVGVQYELTLNGLKFDVLHDSIKEGASGEDGYPHEFKISSIEILPFFNKN
                     FDRNSEGEIIIPDGSGAVISFNSDKQDQFVAGYQPKKIYGFDKSQILRTAPADVQTIM
                     LPMYAYLDKTENVGVIGIIESAPSHHSIVADFMRTRTAGSSNYVNYEVSMRDSEFVNV
                     GRSWYVSSYQKWSQSMIPTDLSYYFQFIVPETIEDGEVKSNANFNYVEVAKQYRRYLI
                     NKYDLQEIDKTKTNVVNLNFLGAFEKDTMTAGFVYQKTHSLTTFEEAQEIIEILKEKG
                     VENFSVSYTSWTKDEMEPKARKDIRISNVLGGTKGFKKLSEFLTENNINFYPEVNVST
                     NQGYDYPFGMKKYNAKSVSNTETVYYPYVVPTGRFDRSLAPIYHISPRFYQSYFNKYL
                     ANYEKLNTSGIYLSDLGNLKVSSYDNKKEIFTVVGQDYQIATLDIAKESGKKVMLKNP
                     FDYALQYANIIVDAPVTTTLYPAVEHSIPLYQLALSGLVDYTTTTVNYNNQQSYRWYL
                     LKALETGSNLNFVISYADTNTLLTTDYTEYFNTYFLNWENKIVEMNQRINEAGIHQGR
                     LVDHKYITDNVVQSTYANDFGVVCRVIINYANSQYLDSINGISIAANDYVILEGE"
     gene            complement(8337..9062)
                     /locus_tag="PLQ22_01450"
     CDS             complement(8337..9062)
                     /locus_tag="PLQ22_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOH58659.1"
                     /translation="MEGKQIMTTEVSKKEKFLNIAKLVLSKITQFFKFLWNEGVKFPV
                     YILFQPFKGFDEFKRYKRAKMSLAITYIVLYILLSIMDFHYSGFIVNQRDITDLNTIA
                     EIGYVVVPLVVLVVANWSVTTLFDGKGTMKDIFLLTSYALFPMIVTKFFGIFVSNIIV
                     AKEVGIYSLIMGIGAFGSAYFLFVGWMAIHEYGLLKCILMAVFTLVAAIVILFAVALL
                     FDLYRRIFSFVYTVYREISLRYF"
     gene            complement(9062..10843)
                     /locus_tag="PLQ22_01455"
     CDS             complement(9062..10843)
                     /locus_tag="PLQ22_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58660.1"
                     /translation="MKKVFYLLIALLLLGSAVVSVNAKGGYTYDHKGTAIHSTVGMSA
                     STDGIYTITSNAWEGIPADQFTSPEDLFIFKHPVTGEETIYIIDSISNHLYVFDSTLT
                     LKQGSDASRRTRRYRLKGETVDRFYAGEIITRYEFRPDTITRYEYDRVKSGTDTGNEL
                     FKNKVDWLTFTAPLQTPVESRGDSQRWYIQPKNISGVYRALRPLRDEGGFIIEGEYQD
                     VLYICDKGNNQILLLDPVDYHVIQVVCQPESIVFEGKTFSPLKMVTDSTGRMFVISES
                     VYEGIMQMSYFGEFMSYIGVNYTTLSAWDKFWRNFSTEEQLKQQTAIVNTEFRNLSID
                     DAGFIYTVSRATKISTYSEDATTMIKKINPSGKDTLVKNGYHLPVGDIAFTLEGSSAS
                     DGISISSRFSGIAINDYGVYTVADEKAGRLFTYDDEGNLLYISGNSGPELNNINSPTA
                     VRYQGENILVLDKYNKAVIRFEPTDIARIINQAVKYQYNGQLAEAAEEWKNVVSENPN
                     YELAYIGIGKSLLSENRYQDAMANFRLGFETSYYSNAYQRYRDREIKLNFTAFAVLAI
                     GAALILVARSKIAELPRKDIIRIVGED"
     gene            complement(10847..11842)
                     /locus_tag="PLQ22_01460"
     CDS             complement(10847..11842)
                     /locus_tag="PLQ22_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH58661.1"
                     /translation="MAEARKKLTAAEKAQLKEDKKALRNAKRAKRIDSRPFAEISTIK
                     VHRRSKKRKRINRSVGGDLVLFFFLCLAGILSALPLVLVINNAFKPLDELFKFPPNII
                     VRNPTLDNFTDLSVALANSTVPFARYLFNTVMVTILGTVGHVVIASMCAYPLAKYKIP
                     GGTIIFTIIVYSLMFPGAVTSIPNYIIFNWIGLIDNPLALILPMIGSSLGLFLMKQFM
                     SQVPTSYIESAKLDGAGDFKIFWTIVMPLVKPAWITLIILVFQSLWNTQGAMYIYRED
                     FKMVSYALSQIATAGAARSGTLAAVSLIMITVPITMFIISQSSMLETMATSGLKD"
BASE COUNT         3958 a         2180 c         1588 g         4200 t
ORIGIN      
        1 atctatagat gcaaaaaatc tcttattatc ttctcttgtt ttctcagcta acttaactac
       61 aaatgtttct tgagtaaagg cagtccctgg ttcattaaaa ccaatatggc cattaccacc
      121 aattcctaat aactgtaaat caacaggatt tttctttaat aaatcattat actttttaca
      181 gttagcttct aagtcatctc cttcagatga aggtaaatga gtatttgctt ttttaatatc
      241 aatgtgttta aataagttct cattcatata agaataataa ctttgttcat gattcttatc
      301 taactgacaa tattcatcta aattaaaact ctttatatct ttaaaagaaa tctctttagc
      361 tttgtacttt ttaattagat ttttataagt acctaaagga cttgaaccag tagctaaacc
      421 taaattagct ttagggtttt tcttaacaac atcagctatt atttcagcgg cttttttact
      481 catttcttca tagtctttaa ctctaataat tttcatattt cctccttcta atttaatcgg
      541 caagcttcct ttaaacttac cgctatatat taattttact aaacttttca aagaaagtaa
      601 agttgcttca tataagcaaa tataactctc aacattttct aaatgtaaga tatcataggg
      661 cgatctcata ctaactacaa ttgttttatt tggatctaga acttgccaaa ctttagtttg
      721 ataatcccct ttagttacat tataagtaca aaaaattact ttatctgctt tcctaatctc
      781 atccttaatt aacttatatt cattaaattc ttgatttatt ataacttcat tacaatccaa
      841 gaaatcttgt aaagtaaaat aatcattttc tttgttatca actaaactaa atagtttaat
      901 ctccggaaag attgcaaaaa tcttttcttg tttttttatt ggaactagcc ccttatcttt
      961 tactaaagtg attgattcta aagcaaaatc ttctgctagt ttagattttt cttccctttt
     1021 aataacactt aaattgtttt caaaagctaa atctttttcc aaataaattt ctttgtattc
     1081 taaaatcttc ttaactgctt catctactct tgaaagagga atttctcctt cttctactaa
     1141 actaacaaag gtattataga tttctttttg ttcatttaaa tcagccttac cgcagaaaca
     1201 taaaatatca tttcccgcta agactccttt cttgacaatc tctttaatac tatagttatc
     1261 ccaaatagct ttcattgtta atgaatcagt tacaactagt cctttaaatc ctaatttttc
     1321 tttaagaagt ttagtaataa tgttataaga caaactagca ggatcgtctt tatctaaaca
     1381 ctgataaaca atatgcgaca tcattattcc attagcccca ttttcaatag cttttttaaa
     1441 aggataaagt tctacttttt caatttcttc taaagactta tctactactg gtaaacctaa
     1501 atgactatct acattcgtat caccatgacc tggaaaatgt ttaacagttg caattagctt
     1561 agcatcttta aatccttgac ttgctcttaa aacatacctt gaaacaagtt ctggattatc
     1621 agaataactc cttgagttaa ttacaggatt ataaggatta ttattaacat ctgctacagg
     1681 agcaaaatta atactaaaac ctaaatgttt taagttttta cctgcgcttt tatataactc
     1741 atatggatca tttccattag ctgcaaaacc catcgccgaa agaagtggtg ttattccttt
     1801 ttcaattctt tgaactggtc caccttcttg atctaaagca ataaatggcc taatcttttt
     1861 atcttgagaa atctcgccat ttaatttgct tacttgcttt aaagaagtaa tgtttctttt
     1921 aaataaaatc aagcccccaa gctctaactc atttaaaaga taatctactt gttcattata
     1981 actagttcca tgaaaactaa acataagcat ttgtccaact ttttgtttta aacttaaatc
     2041 ttttagcttc atcaaatcct ccttaaacaa aagaaaggtg ctattcataa aatagcacct
     2101 tttatttatc ccgtgattcc ggttctttca attccctcta caaattgttt ttgaacgaag
     2161 aaataaccga ttaataacgg tagcatcatt agtagtgctg cagtattcgc aattaatcca
     2221 tagaacatcg gatcggtcgc taaagcttca tcggttaaag tgtcaaagat aggtttacct
     2281 ttggctaccc aaccactaat aaccgtaaac aaacgatctg ttccttcccc taaacgagcc
     2341 gctaataccg gatatgattg aacgatacta aacaatctcg caaagtaata atcattccat
     2401 tgccatacaa aagcaaacaa tgccacaaca actatcgctc ctctagcgtt tggtaacatt
     2461 acactccaga atgttctaat aacaccagca ccatctactt gcgcagattc ctctagttct
     2521 gctggtatat ttctaaagaa ctgtctgaaa agataaatga aaatcgctga tcttattcct
     2581 tgaccaaatg ctgataggat aaatatggat agtactgttc ccatcaactt tataccgaaa
     2641 aatgttgtat taactaagaa taatgatctt gaaacatgta aagtctcatt cggtacaact
     2701 aaggtaaaga gaataatata aaagagaata ttacttcctt taaatcttaa cttagcaaaa
     2761 gcataacctg ctattgctga tgccataact tggataaaca ttattgctaa agaaagaagt
     2821 gtagttctta ccaaagtagg ccaataatca agtaaatcca tagctaaccc aatattagct
     2881 gtactaaatt cagatggaat ccaaacaact tgaggattag ttaagtcgtt aggtgatcta
     2941 aaagcataag agaatttttg gaaaatcggt aaaagaatta caaaacacaa tccgattaaa
     3001 ataagtgttc taaaaattct accagctcta ccaccaactt tttgggcata aacttttctt
     3061 cttgtctttt tttcatgctc agttaaaaca ggtttagaat aatattcttg tggtactaca
     3121 attttttgtt tactcgtcat aatagaacac cacccttgat attacaatca atactaagga
     3181 tattagtatg attgatgata aacagaataa ccagctcata gctgcatgaa taccatgagc
     3241 agtaaccata cctaaaccac cagttaaggc tacaaagtta tttactgagt aataatctaa
     3301 aatacttaag agatcacttc gtaagaagga gtcaacaaca gtataaacac ctgccgtaag
     3361 cattaatggt gaaaccattg gaaaagtaat tttccagaag atttcatatt gagttgcccc
     3421 ctccatttta gctgcttcat ataaatgttt aggaactgat tgaattgcac ttaagaaaat
     3481 taagatttga acccccgaat atgtaattac atcataaatc gaggacgtta cgttgcccaa
     3541 gaaggttaca acacctcccg gaactcccgc ttcgagtaag aagaaactaa agtcaaatcc
     3601 accttgaaac attgtacttg ttgtttctgc agtagcagct gttaatgctg ctcctgtttt
     3661 cattgcaact tcaactgctt gtgagttaaa gataactggc ataaagaaga ctgctcttac
     3721 aaaactacga ccaggaaatt gtgtatttaa aacaactgca ataattaaac tgaaaattaa
     3781 tactaatggt acttcaagta aagtattacc aaatgaagtt aacaatcttt gcctgaaagt
     3841 agaatgttga ttccaaacaa attgatagtt atcaaaacca atattttcag taataactcc
     3901 catattatct cccgtaactg gatttaatcc tatcgtttga atatgagaaa aactatagaa
     3961 aacagttgta ataaacggaa ttaagaagaa ataaccaaaa ccaataatta gcggaattac
     4021 aaaaacaact ccccataaag ctttcttttt tctatgagtt aaatttctag ttagttttct
     4081 tggaacaaaa gataaagctt cttttacctt tacaactggt tttgaaatgc taatcttatg
     4141 gaaaaagtct tgaattgccc atttagcttt tttataccaa gtaactttgt ctcttttagc
     4201 ttgggctttt tgatcttttt gaagttgctt ttgcatctcc ttttctcttt tggcttggat
     4261 tttatcttgc tttgcttgtt cttctaaagc aatataatat cttctcctag caaaatagtc
     4321 attccatttt tctttgaagg tttttttagc ttgattttga ttcataatta ttctccttcc
     4381 aaaatgacat agtcatttgc agcaatggaa attccattta tgctatctaa atattggcta
     4441 tttgcatagt taataataac tctacaaaca acaccaaaat catttgcata cgtagattga
     4501 actacgttgt ctgtaatgta cttatggtct actaatcttc cttgatggat tcctgcctca
     4561 ttgattcttt gattcatctc aacaattttg ttttcccagt ttaggaagta agtattaaag
     4621 tactctgtat aatcagtcgt tagtaaggta ttagtatccg catatgaaat tacgaagttc
     4681 aaattcgaac ctgtctccaa agcttttaat agataccaac gataagattg ttgattgtta
     4741 tagttaactg tagttgttgt atagtcaact aatccactta atgctaattg atataaagga
     4801 atcgaatgct ctactgctgg atataaagtt gtagtaactg gtgcatcaac aataatgttt
     4861 gcatattgta atgcgtaatc aaacggattt ttaagcatta cctttttacc agactcttta
     4921 gctatatcta atgtagctat ttgataatct tgaccaacaa cagtaaagat ttctttttta
     4981 ttatcatatg atgaaacttt taagttacct aaatcagata aataaatacc cgaagtatta
     5041 agtttctcat aattagctaa gtatttatta aagtatgatt gataaaaacg aggtgaaata
     5101 tgataaatcg gagcaagaga acgatcaaat ctaccagtag gtactacata aggataatat
     5161 acagtttctg tattacttac actcttagca ttgtactttt tcatgccaaa tggatagtca
     5221 tatccttggt ttgtagaaac attaacttct ggatagaaat taatattgtt ctcagttaag
     5281 aattcactta acttcttaaa tccttttgtt ccacctaaaa cattagaaat tctaatatct
     5341 tttcttgctt ttggttccat ctcgtcttta gtccatgatg tataactaac actaaagttt
     5401 tctacacctt tttcttttaa gatttcaata atctcttgtg cttcttcaaa agttgttaga
     5461 ctatgtgttt tttgataaac aaatccagcc gtcattgtat ctttttcaaa agcacctaag
     5521 aagtttagat taactacatt agtcttagtt ttatcaattt cttgtaaatc gtatttgtta
     5581 attaaataac gacgatattg ctttgctacc tcaacataat taaagttagc atttgatttt
     5641 acttccccat cttcaattgt ttctggaaca atgaactgga agtaatatga taagtccgta
     5701 ggaatcatcg attgcgacca tttttgatat gaagatacat accatgatct tccaacattt
     5761 acaaactcag aatctctcat acttacttca tagttaacat agtttgaact acctgctgtt
     5821 cttgttctca taaagtcagc tacaatacta tgatgacttg gagcagattc aataataccg
     5881 ataacaccaa cattttctgt cttatctaaa taagcataca ttggtagcat tattgtttga
     5941 acatcagctg gtgctgtacg taagatctgc gatttatcaa aaccataaat cttttttggt
     6001 tgataacccg ctacgaattg atcttgttta tcactattaa agctaattac agctccgcta
     6061 ccatcaggga taataatttc tccttcagag ttacggtcaa agtttttatt gaagaaaggt
     6121 aaaatttcaa tacttgaaat tttaaattca tgaggataac catcttcacc actagcacct
     6181 tctttaattg aatcgtgtaa aacatcaaat tttaaaccat ttaatgtaag ctcgtattga
     6241 actccaactt ggaaaatcgc acttgctgaa gcagcacttt gttgataaat cctattttgc
     6301 tcttctacaa tatttcttct ttcttcgcct tcagctgcca aaaatggttc actaaattga
     6361 gcacgaactg gattacctag ttgatcataa acatagtttc cttctgcgtc tcttagtgga
     6421 actccacctt gaactactgg tctaccttca taataaacaa ttttactatc atcatattta
     6481 tctataactg aagtactagt tctcatatag ttagtcggac taatatcata taagaacata
     6541 tataattcat ttcttcttgg ttctgatgta gctttgtttt cataagtctt agaactattt
     6601 gctgtatagt gaatatagtt tttatttgtt tcattagtag ttatgttata gataagttca
     6661 tagttaccat ccataattga cttaatatta gccgctgtta agaatgggtt aacacgaata
     6721 ggagaatctt tagtattgca gtttacccct acaactaact ttggttttcc cactttgtct
     6781 atagttactt taccatcttc ataatctgct tctgtccaaa gcaatccact taaatcccaa
     6841 tagcctaaga aagtgttagt accatcagca ctataagctg gttttgcttt acctaagcca
     6901 tttgcttcaa tataattagc agcttcttcg tttgttgtaa caccattttc acgataaatt
     6961 aaattaaaat gaccttgtga atcacgctca gatgatacaa cttttgtaat ccaagtatta
     7021 cctctaacta tatcttcaaa gactgctctt tcaatatatt tagggaagaa gttgttagca
     7081 ttagtaaatt caccaacttc atataaaact tgaactttat tatccccagc ataattaatc
     7141 ttgtagtgtt ttgtagccat acctttaatt aagtccatat aagctacact ctttgaatat
     7201 gttcctaagg ctgtagctga aacttttcca cttcgaccaa aataacgaag cctaaaaaca
     7261 tcaccactaa ctaattcgcc tctttcattt gtgtaatcgc ttaattgata taaaacatta
     7321 aatgattcat atactaaaaa gccattttca tcttgaactt gaagtccacc aactttcttt
     7381 ggtgttccag ttgaatttaa accaatcgaa atagccgttg ttttttcatt aatatacatt
     7441 gtaaactttt cattttgagc tacaattctt tcttcctcaa aaattgatgt tttatattca
     7501 atgttatcta aaactgagtt taaaacaatt gactcagcaa tatcatttaa aataccgact
     7561 gctctttgta tatcttgacg aatatttgcc agtacttctt catcaggatc tggaattgat
     7621 ttttgaacta ttgctagttc tgacatctta gtttcaatta actttattgg ataatcaaaa
     7681 acaatagtgt gattatcaaa atctatatta gtattattgg gaagaacgat ttcttgatct
     7741 tccatttcac cttcttctcc accttcttta ggtacttgaa taatagctgt ttcgtaagta
     7801 tcaaagaagt ttaatgcagt taatttatct acaatctctt gaaaagcagc tttgcctttg
     7861 tctgttgttg ctacaatttt taagttgtca actcctggtt cacctaaatt atcttcatag
     7921 atgtccatat caaaatcacc agcttcaaaa agtctagcaa tagcaagttg agcaatatac
     7981 atttcattag caatatcttc ttcattataa ccgacattta aattaaggta gattgctgta
     8041 ttgccatagt aatctgtttt ttcaactttg taacccgcag tctttaatgc attagctaca
     8101 ttgtcaatat cacttgcagg aaatcctgcc ttaaaagtaa ttttacgatc atattgttct
     8161 agatgagaaa aatcaagcaa aaattcttca gaattgaatt cttcattatc atagctgata
     8221 gttgttgctt ttaggccacc aataaagtaa acacctaaag taatactaat tgctataact
     8281 aatagaccta aaacaatatg tcttaatcta ataattttca acttattttc ctccttctag
     8341 aaatatcgta aggatatctc tcgatatact gtgtatacga aactaaagat tctacggtat
     8401 aaatcaaata gtaaagcaac agcaaataaa attacgatag ctgcaactaa agtaaacact
     8461 gccattaaga tacattttaa taaaccatac tcatgaatag ccatccaacc aacgaaaagg
     8521 aaataagcac taccaaaagc tccaataccc ataattaagc tatagatacc tacttcttta
     8581 gctacgataa tattacttac aaaaattcca aagaatttag taactatcat tggaaataga
     8641 gcataactag taagtaagaa aatatctttc atcgttcctt taccgtcaaa tagtgttgta
     8701 actgaccagt tagcaactac taaaacaact aaaggtacaa ctacatatcc tatttccgct
     8761 atcgtattta aatcagtaat gtctctttgg tttacaataa aacctgaata atggaaatcc
     8821 ataatactta aaagaatata aagaacaatg taagtaatag ctaaactcat ctttgctctt
     8881 ttatatctct taaattcatc aaatccttta aaaggttgga ataaaatata aacaggaaac
     8941 ttaactcctt cgttccataa aaacttaaag aattgtgtga ttttagataa aacaagtttt
     9001 gcaatattaa gaaacttttc ttttttagaa acttcagttg tcataatttg ttttccttcc
     9061 attaatcttc acctacaatt ctaataatat cttttcttgg tagctctgct atcttacttc
     9121 tagcaactaa aattaaagct gctccgattg ctaaaacagc aaatgccgta aagttaagtt
     9181 taatttcacg atcacgatat ctttgataag catttgaata ataactagtt tcaaatccta
     9241 aacggaagtt agccatagca tcttgatatc tattttcgct taataatgat ttaccaatac
     9301 caatataagc aagctcatag ttagggtttt ctgaaactac gttcttccat tcttctgctg
     9361 cttcagctaa ctgaccattg tattgatatt taactgcttg attaataatt ctagcaatat
     9421 cagttggttc aaaacgaata acagctttgt tgtatttatc aagtactaag atattttcac
     9481 cttgataacg aacagcagtt ggactattaa tattatttaa ctctggtcct gaattacctg
     9541 agatgtaaag taagttacct tcatcatcat aagtaaataa tcttcctgct ttttcgtctg
     9601 ctactgtata aacaccataa tcgttaattg caatacctga aaatcttgat gaaattgaaa
     9661 ttccatcact tgcactacta ccctcaagtg taaatgcaat atcccctact ggtaaatgat
     9721 aaccgttttt aactaaagta tcttttcctg atggattaat cttcttaatc attgttgttg
     9781 catcttcgct ataagtagag atctttgttg ctctagaaac agtatagata aagccagcat
     9841 catcaattga taaatttcta aactcagtat ttacaatcgc tgtttgttgt tttagttgtt
     9901 cttctgtaga gaagttacgc caaaacttat cccaagctga tagtgttgta taattaacac
     9961 caatataact catgaactca ccaaagtagc tcatttgcat aataccttca taaacagact
    10021 cagaaattac aaacattctt cctgttgagt cagtaaccat ttttaaaggt gagaatgtct
    10081 taccttcaaa aacaattgat tctggttgac atacaacttg aataacatgg taatcaactg
    10141 gatctaaaag taaaatttgg ttatttccct tatcgcaaat atataaaaca tcttgatatt
    10201 caccttcaat aatgaatcca ccttcatctc ttaaaggtct taaagcacga taaacaccag
    10261 aaatattttt tggttggata taccatcttt gactatcccc cctactttct actggagttt
    10321 gaagtggtgc cgtaaatgtt aaccagtcaa ctttattctt aaatagttca ttacctgtat
    10381 ctgtaccaga cttaactcta tcatattcat aacgagtaat tgtatctggt ctaaactcat
    10441 aacgagtaat aatttcgcct gcgtaaaacc tatctacagt ttcaccttta aggcgatatc
    10501 ttcttgttct tcttgatgca tcgctacctt gtttaagtgt taatgtgcta tcaaaaacat
    10561 ataaatggtt agagattgaa tcaataatgt aaattgtttc ttcacctgta actgggtgtt
    10621 taaatataaa caagtcttct ggagaagtaa attgatcagc tggtattcct tcccaggcat
    10681 ttgatgtaat cgtatagatt ccatcagtac ttgcactcat tcccactgtt gaatggatag
    10741 ctgtaccttt atggtcataa gtatatccac ccttagcatt aacactaaca acagcagaac
    10801 caagtagcaa taaagcaatt agtaaataaa atactttctt cattgcttaa tcctttagtc
    10861 cacttgtagc cattgtttca agcatgctac tttgggaaat aataaacatt gttattggta
    10921 cggtaatcat aattaagctt accgcagcta atgtacccga tcttgcagct ccagctgtag
    10981 ctatttgtga tagggcataa cttaccatct taaaatcttc tcgataaata tacatcgctc
    11041 cttgtgtatt ccataaactt tggaaaacta aaataattaa agtaatccaa gctggtttta
    11101 ctaaaggcat aacgatagtc cagaaaatct taaaatcgcc agcaccatct aacttcgccg
    11161 attcaatata ggaagttggt acttgagaca taaactgttt cattaagaat aatcctaagg
    11221 atgatcctat catcggcaag attaaagcta gtggattatc aattaaacca atccagttaa
    11281 agataatgta gttaggaata gaagttactg ctcctgggaa cattaaagaa taaacgataa
    11341 ttgtaaagat aatcgttcca cctggaatct tatattttgc aagtggataa gcacacattg
    11401 aggcaataac tacgtgtcct actgttccaa gtattgttac cataactgta ttaaataaat
    11461 atctagcaaa cggaacagtt gagttagcta gtgcaacact caaatctgtg aagttatcta
    11521 aagttgggtt tcttacaata atgtttggtg gaaacttgaa caactcatct agaggtttaa
    11581 aggcattgtt tattactaat actagtggta atgcacttaa aattcccgct aaacatagga
    11641 agaaaaatag tactaagtct cccccaactg atctattaat tctttttctt ttcttactac
    11701 gacgatgtac tttaatcgta cttatttctg cgaaaggtcg tgagtcaata cgtttagcac
    11761 gtttggcatt acgaagagct tttttatctt cttttaactg tgctttttca gccgcagtta
    11821 atttttttct tgcttcagcc attatgatcc cacccttcca ataatttttc taattaattt
    11881 gtttaatgct atcgccgaaa caaaaaggat tgtagcaatt gctgag
//
LOCUS       DAOSGS010000017        11479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4746_length_11479_cov_5.692665, whole genome shotgun sequence.
ACCESSION   DAOSGS010000017 DAOSGS010000000
VERSION     DAOSGS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4746_length_11479_cov_5.692665"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLQ22_01465"
     CDS             complement(<1..274)
                     /locus_tag="PLQ22_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58662.1"
                     /translation="MKFLDTTNKIEKVYQKGEFNLELWKKYIATIHPKLGQLCLEDMN
                     KAIETGLVSFKEHYQPILNDVIKNKKAKEEVAKNFYLITQNLDQEII"
     gene            388..1866
                     /gene="lysS"
                     /locus_tag="PLQ22_01470"
     CDS             388..1866
                     /gene="lysS"
                     /locus_tag="PLQ22_01470"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOH58663.1"
                     /translation="MKNLTDQEIVRREKVKELEDLGIKPFGHAYKPTSNSKKLQEKYL
                     EKTKEELEEKKIKVKVAGRIMTKRRSGKAGFMHIQDKFGQVQIYIREDEIGEEQYYLY
                     RRCDIGDIIGIEGFVIKTNTGELTVRATKYVHLTKALRPLPEKYHGLQDVEEARRHRY
                     VDLIMNEESRYIAFLRPRIIRLVQHFFDSRGFVEVETPILHSVLGGANARPFITHHNT
                     LDMDFYLRIATELPLKKLIVGGMERVYEIGRLFRNEGMDSTHNPEFTTIEAYQAYSDM
                     VGMMDLVEELFQYLAKETNGSYIINYQGHELDFTKFKRVHMADLIKEETGIDFFKVKS
                     DEEALKLAKKHDIQVKEHEKSFGHIVNLFFETYCEEKLIQPTFVYGHPLDISPLAKKN
                     DKDPRFTDRFELFINTKEYANAFSELNDPIDQKERFEKQLEEKSKGNDEASEMDTDFL
                     EALEYGLPPTGGLGIGIDRTCMLLANVDSIRDIILFPHMKNK"
     gene            complement(1897..2421)
                     /locus_tag="PLQ22_01475"
     CDS             complement(1897..2421)
                     /locus_tag="PLQ22_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58664.1"
                     /translation="MTNRDDYKAYLEENKELLTTLKKHNTLTYFRIANLIKVLNYILE
                     SKKIDKIYETIFDVGFNFLHATLEEIKEYLDIYFDNDYEAFIKQELYVNYILILDDLR
                     LSIKEQTKLDEEDEEHILKMQESLETYLKKGLDVPKKVYREYQDYVQTLSHKYSDVRL
                     TVEVFEEIHDKLMY"
     gene            complement(2411..3013)
                     /locus_tag="PLQ22_01480"
     CDS             complement(2411..3013)
                     /locus_tag="PLQ22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOH58665.1"
                     /translation="MERIIIRMLFDKKINKKYYRFHLFRKSFTFYLILLTGLFVVYLA
                     VKNTFMATDSEEVQSLSSMIMIWGLAFFALVLAPSFMFWRINSLVKKEAKKRGDKEEY
                     ISITKEKIERRIDGQDKLVFGWNNIVDIRELEDMFLLYVDTESAVVVSKHHMIEGSPE
                     VLRSLIEKYGPKDKKGKLKYKVHMKGYKKPKKVKKEKNDK"
     gene            complement(3022..3573)
                     /locus_tag="PLQ22_01485"
     CDS             complement(3022..3573)
                     /locus_tag="PLQ22_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58666.1"
                     /translation="MFELIKKAEQEHTIIEITVTNVGGPTLLTGYVLKVDESYFIFED
                     YLYNGKFAGYVLGKIDDIVRVEIYDNQLEILEKLIKHYNYDPLKITLEDDDLLLLSFI
                     NFALNNNLVIGYGVEDYNGNIKYGTIQSFDEGIIKVHSYNISGESSGVDYIELFHFKT
                     IRVLGINEERVSILRALNNRNYQ"
     gene            3655..4470
                     /locus_tag="PLQ22_01490"
     CDS             3655..4470
                     /locus_tag="PLQ22_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOH58667.1"
                     /translation="MDCLIKSVAYNNNVRVYLVSIKELLDERLSLNKYSENGKELFGK
                     VLSASLLLGGGLKNDQRLSIKIKGDGECELIVTEVDSLGNIKGYIANPNCLEGKPLDE
                     EKALGNGQLTVTKDLNIKKLYTTSIPLNYPLIQDNFTYYFLKSEQVMTFVLLAKIGGV
                     LIQLLPGYDPKIIDSLKEVIEEYYDIEKYLDKYPPKDILKLMFEDLDILDIVNVKYYC
                     DCSKKKFANGLKTLPKEELEEIIKEDGKAEIVCNYCNKKYHFSKEELEDLIKK"
     gene            complement(4483..5025)
                     /locus_tag="PLQ22_01495"
     CDS             complement(4483..5025)
                     /locus_tag="PLQ22_01495"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH58668.1"
                     /translation="MIVLGSSSPRRKELLAMLGLEFIVYSPNIDENFNLKDPIALSEK
                     TALEKGKAVSLVYPNATVIAADTIVLIENEILGKPKNKEDAFKMIKEIQGKVHYVITS
                     VYLSKGSNYKVFSEKTKVWVSKMTDKEIEDYVSTKEPYDKAGGYAIQGLFAKYVERID
                     GDFYNVMGLPLNRVYNELKK"
     gene            complement(5037..5291)
                     /locus_tag="PLQ22_01500"
     CDS             complement(5037..5291)
                     /locus_tag="PLQ22_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOH58669.1"
                     /translation="MRLDKFLKASRLIKRRSVAKKASEDELVMVNDRIAKPSKEIAVG
                     DIIKIRYAKTTIVLKVLSLNQSIKKEDASSMYEIIEKIDN"
     gene            complement(5281..7323)
                     /gene="ftsH"
                     /locus_tag="PLQ22_01505"
     CDS             complement(5281..7323)
                     /gene="ftsH"
                     /locus_tag="PLQ22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH58670.1"
                     /translation="MQRKFKFSNILLILILLLLIPGIIMLVNGLTNKPEKWSYDQIYA
                     RTEDGVDEFTTIAIKPETGNNLGLWQMKGTYKDAEGNVKAFVVVLREAQLEEIENKLL
                     LNPQITIENVTVSNVDWFSIIVWIVLPIVLIGAAIYFLTKTAGGASSSNKQAFDFGKS
                     RAQLSKKSKVTFNDVAGCDEEKEELVEVIDFLKNPKKYFEIGARVPKGILLTGQPGTG
                     KTLLAKAVAGEAKVPFYSISGSDFVEMFVGVGASRVRDMFKNAKQNAPCIIFIDEIDA
                     VGRQRGTGLGGGHDEREQTLNQLLVEMDGFEENSGVIVMAATNRADILDPALLRPGRF
                     DRQIRISLPDVRGREAILKVHARNKKLSPRINLENIAQRTPGFSGADLENLLNEAALL
                     AARENRKEIKVYDIDEAIDRVLMGPAKKSRKYSDEEKRTVSYHEAGHAVIGLKLDNAN
                     VVQKVTIIPRGDAGGYAMMTPEEEKFLQTRKGLIDTITGLLGGRAAEELMCNDMTTGA
                     SSDIERSTAIARAMVTRFGMSRLGPIQYERGGQEVFLGRDYLAEKNFSDQVALEIDKE
                     VQAIIDESHKKATKILKESKDLLELIASHLYEIETLTKEDIYELLETGKLAWWEKKKA
                     RQEAERIAKEKEAELQEIYNKQLESMAQYKKEQEAEVAKLKEEPKEEEKPEPKDEA"
     gene            complement(7327..7866)
                     /gene="hpt"
                     /locus_tag="PLQ22_01510"
     CDS             complement(7327..7866)
                     /gene="hpt"
                     /locus_tag="PLQ22_01510"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH58671.1"
                     /translation="MKKIVEEVLLTNEEIKNRCKELGEEVTKDYAGKRLVLICLLKGS
                     VPFATELSTHINLDLEMEFLRASSYDGTNSKGQVKIDYLFTGNLEGKDVLIVEDIIDT
                     GLTLDVVVRELKKMNPKSLEIIALLNKKDVNTGLKSKPKYVGFDIPNKFVVGFGLDYN
                     ELYRNLNFIGVLKKEVYGG"
     gene            complement(7850..9169)
                     /gene="tilS"
                     /locus_tag="PLQ22_01515"
     CDS             complement(7850..9169)
                     /gene="tilS"
                     /locus_tag="PLQ22_01515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH58672.1"
                     /translation="MDKVISAFFEEKKLELKNKKVLIATSTGVDSMVMLASFLRLRKE
                     YKLDLYVAFVNHLKRKEANLEEEYIKSFCKENEIKLAVERLDYLDESENFQKEARKLR
                     YQFFEKVIKKEKIDYLVLAHHANDNLETILMRILRGSNLAGYAGIESVVKYPGYTLVR
                     PFINLEKEDIYRYALRENVKYFEDKSNIDTIYTRNKIRSLIDPEILKVNEQGVYKLEE
                     YGETLKAAWRIVSKIVDEFIEKKVSNLSFAREDFLALDSYIQEEIIFKLLKKFNLSKE
                     NVLEIISLISSDKANIKTYFKNSFTFYKEYDKITIVPYLKKELDFELIIDKENFYYED
                     NKLEVKSKKDNLIDSKYFNICYNMDMFPLKLRTRKDGDKIKLKSGIKKVKDYLIDQKI
                     GILEREDILILEDKDSNVLSIVGLVNSSLTKTCDYSILIRRINEKDS"
     gene            complement(9281..11365)
                     /locus_tag="PLQ22_01520"
     CDS             complement(9281..11365)
                     /locus_tag="PLQ22_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOH58673.1"
                     /translation="MEKHIYRNKFIVFSLVFFVLNIINSYLSSWTKVNALPSAYLRDS
                     FMVVNSIIGDAAFFAVFYGLGILIFKSDNARYRYMIVLTTFFSLLYFALSVFISNYGM
                     FFSFWNLDLFTRDTGASESFVFVLQSIFIIIRGGKIIFFIPIIILTFLYYYKFIRKEE
                     EKTFSSSFPGFRRWLGGFVIAFLGIFFMGSSYVSYKEVNKGTWYEDNASALYAAESMG
                     VFNYYVFEAFDYAFSEKIPEKDDLIEEKIAELKIKLEQHQYVNQINILDGQNYGPNLE
                     YKGIYEDKNLILIQAESLNQFVIGLEVLVDGDYKELTPNINKMLEKSIYFNNFYTAAG
                     IGNTSDSEFTVLTGLYPTGNKYTVFNHVGVDLPTLAESFKNEDYVTKSYHANVGYYYD
                     RKNVHVNSYNFDEHISEEDLKELGVYDENRIVHRWISDLDLLRYVASDVKTINDQGKK
                     FFGMPITISTHMPYEEDLNLFLEKDRFFSADYSSIEYPLLGYLEHISYMDFCFGQFLA
                     ELETLGVLDNTIIAFYGDHGNSIDFLDTMVPNANLFRNKFRTMYEIELGRKDLERRFV
                     QRMLLSRVPFVIYDHNRTEAPVIQDKVRNTTVVSRTISNLFNLKQEYYFGIDGLSNQP
                     FFAYCPRNNNIYIGDMIISSQSGEYFDCDDVVNYDAYRRNLIIKKYKDLKDFNDKLLE
                     LKVFPALEEAEE"
BASE COUNT         4004 a         1998 c         1347 g         4130 t
ORIGIN      
        1 aaattatttc ttgatctaaa ttttgagtaa tcaaataaaa atttttagcc acttcttctt
       61 ttgctttttt gtttttaata acatcattta aaattggttg ataatgttct ttgaaagaaa
      121 caagcccagt ttctattgcc ttgttcatat cctccaaaca taactgtccc aacttaggat
      181 gaatagtggc aatatatttt ttccataact ctagattaaa ctcacccttt tggtaaactt
      241 tttctatttt attagttgta tctaaaaact tcattttaac acctctgctt actactttac
      301 attataacac aaattaccct tttattaatt gaaaacctaa gtaaaatatg gtataattac
      361 taaagcaata agaggtgaaa acttatgatg aaaaatctaa cagatcaaga aatagtaaga
      421 cgagaaaaag taaaagaatt agaggactta ggaattaaac cttttggtca tgcctataaa
      481 ccaacttcta attctaagaa attacaagaa aaatatttag aaaaaactaa agaagagtta
      541 gaagaaaaaa agataaaagt taaagtagct ggtagaataa tgactaaaag aagatcagga
      601 aaagccggct ttatgcatat ccaagacaaa tttggtcaag ttcaaatcta tattcgtgaa
      661 gatgaaattg gagaagaaca atattactta tatcgtagat gcgatatcgg tgatattatt
      721 ggtattgaag gttttgtaat taaaacaaat acaggagaac taacagtaag agctactaaa
      781 tatgttcacc taacaaaagc tttaagacct ctaccagaaa aatatcatgg acttcaagat
      841 gttgaagaag caagacgtca tcgttatgta gatttaatca tgaatgaaga gtctagatac
      901 attgctttct taagaccaag aattattaga cttgtccaac acttctttga tagtaggggg
      961 tttgtagaag tggaaacacc gattttacat tctgtcttag gtggggctaa tgctagacca
     1021 tttatcacac accacaatac tttagatatg gatttctact taagaattgc tactgaactt
     1081 cctttaaaaa aactaatcgt aggtggtatg gaaagagtat atgaaatcgg tcgtctattt
     1141 agaaatgaag gaatggactc tactcacaac ccagaattca ctacaattga ggcttatcaa
     1201 gcatattctg atatggtagg aatgatggat cttgtagagg agttattcca atatttagct
     1261 aaagaaacaa atggttcata cattatcaac tatcaaggcc atgaattaga ctttactaaa
     1321 ttcaaaagag ttcatatggc agacttaatc aaagaagaaa caggaattga tttctttaaa
     1381 gttaaatctg atgaagaagc attaaaatta gctaaaaagc atgatattca agtaaaagaa
     1441 cacgaaaaat ctttcggtca cattgttaat ctattctttg aaacatactg cgaagaaaaa
     1501 cttatccaac ctacttttgt ttatggccat ccactagata taagtccatt agctaaaaag
     1561 aatgataaag accctcgctt tactgaccgc tttgaattat tcattaatac taaagaatat
     1621 gccaatgctt tctcagaatt aaatgatcca attgatcaaa aagaaagatt tgaaaaacaa
     1681 ctagaagaaa aaagcaaagg taatgatgaa gcaagcgaaa tggatacaga tttcttagaa
     1741 gctttagaat atggcttacc accaacaggt ggtttaggaa tcggtattga ccgtacttgt
     1801 atgttacttg caaacgtaga ttcaattagg gatattattt tattccctca tatgaaaaac
     1861 aaataaaaaa agaagcaact tattaaagtt gcttttttaa tacattaact tatcgtgtat
     1921 ctcttcaaat acttctacgg ttaatcttac atcactatac ttatgactta aagtttgaac
     1981 atagtcttga tattccctgt atactttttt tggaacatca aggccttttt ttaaataggt
     2041 ttctaaactt tcttgcatct taagaatatg ttcttcatct tcttcatcta atttcgtttg
     2101 ttctttaatc gataatctta aatcgtctag aattaaaata tagtttacat ataactcttg
     2161 tttaatgaaa gcttcataat cattatcaaa atagatatct aaatactctt ttatctcttc
     2221 taaagttgca tgaagaaagt taaatcccac atcaaaaatt gtttcataaa ttttatcaat
     2281 cttcttgctt tctaaaatat aattcaaaac tttaattaaa ttggctattc taaaataagt
     2341 aagagtatta tgttttttta aagttgtaag taattcctta ttttcttcta aataagcttt
     2401 ataatcatcc ctatttgtca tttttttcct tcttaacttt ttttggtttt ttataacctt
     2461 tcatatgaac tttatatttc aacttacctt ttttatcttt aggaccatac ttttcaatta
     2521 aactacgtaa aacttccgga gagccttcaa tcatatgatg tttagaaact acaactgctg
     2581 actcagtatc tacatacaat aaaaacatat cttctagttc tcttatatct actatattat
     2641 tccaaccaaa gactaattta tcctgtccat caattcttct ttcaattttt tcttttgtaa
     2701 ttgaaatata ctcttcttta tctccccttt ttttagcttc cttcttaact aaagagttaa
     2761 ttctccaaaa cataaacgaa ggagctaaga ctaaagcaaa gaaagccaaa ccccaaatca
     2821 ttatcatact acttaatgac tgtacttctt ctgaatctgt tgccataaag gtattcttta
     2881 cagctaagta cactacaaat aaaccagtaa gcaaaattaa gtagaatgtg aaagacttac
     2941 gaaataagtg gaacctataa tacttcttat taattttctt gtcaaataac atcctaataa
     3001 ttattctttc cattatacct cctattgata atttctatta ttaagagctc ttaaaattga
     3061 aactctttct tcatttattc ccaaaactct aattgtctta aaatgaaata attcaatata
     3121 atctactcct gaactttccc cagaaatatt atatgaatga actttaataa ttccttcatc
     3181 aaatgactga atagttccgt atttaatatt accattatag tcttctacac cataaccaat
     3241 aactaaatta ttattaagag caaaattaat aaaacttaaa agcaataaat catcatcttc
     3301 taaagtaatt tttaaaggat cataattata atgttttatt aatttctcta atatttctaa
     3361 ttggttatca tagatttcta ctctaacaat atcatctatt ttccctaaaa cataacctgc
     3421 aaacttacca ttatataaat aatcttcaaa aataaaataa ctctcatcaa cttttaaaac
     3481 ataaccagtt aaaagtgtag gtccacctac attagttact gttatctcaa taatggtatg
     3541 ttcttgttcg gcttttttaa ttaattcaaa caaaattact cctcctcata cattatataa
     3601 ttataccaca aaatcattat ttatggtata ataaataaca aagaaggtga gattatggat
     3661 tgtttaatta aaagtgttgc ttataataat aacgtcagag tctatctagt ttccataaaa
     3721 gaacttttag acgaaagact aagtttaaat aaatatagcg aaaatggtaa agaacttttt
     3781 ggaaaagtct tatctgctag ccttttactt ggtggtggtt taaaaaacga ccaaagacta
     3841 agcattaaga ttaagggtga cggagaatgt gaactaattg taaccgaagt agatagcctt
     3901 ggtaatataa agggatatat tgctaaccct aattgtttag aaggaaagcc tttggatgaa
     3961 gaaaaagctt taggtaatgg tcaactaaca gttactaaag atttaaacat caaaaagctt
     4021 tatacaactt ccattcctct aaactatcca ttaatccaag ataactttac ttactacttt
     4081 ttaaaaagcg agcaagtaat gacttttgtt ttactagcga aaattggcgg agtgttaatc
     4141 caacttcttc caggttatga tccaaagatt attgatagcc taaaagaagt tattgaagaa
     4201 tattatgata ttgaaaaata cttagataaa tatccgccaa aagatatctt aaagctaatg
     4261 tttgaagatt tagatatctt agatatcgtg aatgtaaaat actattgcga ttgctctaaa
     4321 aagaaatttg ctaatggtct taaaacttta ccaaaagaag aattagaaga gattattaaa
     4381 gaagatggta aagcggaaat agtttgcaat tactgtaata aaaagtatca tttctctaaa
     4441 gaagaattag aggatttaat taaaaaatag cacgaattcg tgctattttt ttaattcatt
     4501 ataaactcta tttaaaggta gtcccattac attatagaag tctccatcaa ttctctctac
     4561 atacttagca aataaacctt ggatagcata tcctcctgct ttatcgtatg gttctttcgt
     4621 actaacatag tcttctattt ctttatctgt cattttagat acccaaactt tagtcttttc
     4681 tgagaaaact ttatagtttg agcctttact taaatacaca gatgtaatca cataatgaac
     4741 tttcccttga atttctttaa tcattttaaa agcatcttct ttgtttttag gtttacctaa
     4801 aatttcattt tcaattaaaa cgatagtatc agctgcaata acagttgcat ttggataaac
     4861 taaacttact gctttacctt tttctaaagc agttttttca cttaaagcaa ttggatcttt
     4921 taaattaaag ttttcatcaa tatttggtga ataaacaata aactctaatc ctaacattgc
     4981 taatagttct tttcttcttg gtgaagaaga tccaagtaca atcataaaat taccccttag
     5041 ttatctatct tttctataat ttcatacatc gatgatgcat cttctttttt aatgctttgg
     5101 tttaaagaaa gaacttttaa aacaatagtt gttttagcat acctaatttt aataatatca
     5161 ccaacagcaa tttctttact aggtttagct attctatcat taaccataac taattcatct
     5221 tcgcttgctt ttttagcaac acttcttctt ttaataagtc tagatgcttt taaaaattta
     5281 tcaagcctca tctttaggtt caggtttttc ttcctctttt ggttcttctt ttaatttagc
     5341 tacttctgct tcttgttctt tcttatattg agccatactt tctaattgct tgttatagat
     5401 ttcttgtaac tcagcttctt tttctttagc tattctttca gcctcttgac gagctttttt
     5461 cttttcccac caagcaagtt taccagtttc aagtaactca tagatatctt ctttagtaag
     5521 tgtttcaatt tcatataagt gagaggcaat tagttctaat aaatccttac tttcttttag
     5581 aatttttgta gcttttttat ggctctcatc aataattgct tgaacttctt tgtcgatttc
     5641 taaagcaact tggtctgaga agtttttctc agctaaatag tcacgaccta agaatacttc
     5701 ttggcctcct ctttcatatt ggataggacc aagtctactc ataccaaaac gagtaaccat
     5761 tgctctagca atcgccgttg atctttcaat atcgctagat gcgcctgtag tcatatcgtt
     5821 acacattaac tcttcagctg ctctaccacc aagtaatcct gtaattgtat caattaaacc
     5881 tttacgtgtt tgtaagaact tttcttcttc tggagtcatc atcgcataac caccagcatc
     5941 accacgagga ataattgtaa ctttttgtac tacattagca ttatctaatt ttaaaccaat
     6001 aacagcgtga cctgcttcat gatatgatac agttctcttt tcttcgtctg agtatttacg
     6061 gctcttctta gcaggtccca ttaacactct atcaatagct tcatcaatat catatacttt
     6121 aatttctttt ctattttctc ttgcagcaag taatgcagct tcgtttagta agttctctaa
     6181 gtcggctcca ctaaatcctg gagttctttg agcaatattt tctaagttga ttcttggaga
     6241 tagtttttta ttacgagcat gtacttttag aatcgcttct cttcctctaa catcaggtaa
     6301 agaaatccta atttgacgat caaaacgtcc tggtcttaat aacgcagggt ctaaaatatc
     6361 agctctgttt gtagctgcca taacgataac accagagttt tcctcaaaac catccatctc
     6421 aactaataat tggtttagag tttgttctct ttcgtcatgg cctccaccta aaccagtacc
     6481 tctttgacga ccaacagcat caatctcatc aataaagata atacatggtg cattttgttt
     6541 agcattctta aacatatctc taactctact agcaccaaca cctacgaaca tctctacaaa
     6601 atcagaacca gagattgaat aaaatggaac tttagcctca cctgcaacag cctttgcaag
     6661 taaagtttta ccagttcctg gttgaccagt taaaaggata cctttaggaa ctcttgcccc
     6721 aatttcaaaa tatttctttg gattctttaa aaagtcaatt acctctacaa gttcctcttt
     6781 ttcttcatca caacctgcta catcattaaa tgtaacttta gactttttag aaagttgtgc
     6841 tctactctta ccaaaatcaa atgcttgttt attagaactt gatgctcctc cagctgtttt
     6901 tgttaagaag taaatagcag caccaattaa gacaattggc aacactatcc aaacaataat
     6961 actaaaccag tctacattag aaactgttac gttctcaata gtaatttggg gatttaataa
     7021 taacttgttt tcaatttctt ctaattgtgc ttctcttaac acaactacaa aagccttaac
     7081 atttccttct gcgtctttat aagttccctt catttgccat agacctaaat tatttcctgt
     7141 ttcaggttta atagcgattg ttgtaaactc atctacgcca tcttcagtac gagcataaat
     7201 ttgatcatat gaccattttt ctggcttatt agtaagtcca ttaactaaca ttataatacc
     7261 aggaattaaa agcaataaaa taagtattaa taaaatattc gaaaacttaa attttctttg
     7321 catatattaa cctccataaa cttctttctt tagaactcca ataaaattta aattacgata
     7381 gagttcatta taatctaaac caaaaccaac tacaaattta ttagggatat caaacccaac
     7441 atatttaggt tttgatttta aacctgtatt tacgtctttt ttatttagta aagctataat
     7501 ttctaaagat ttaggattca ttttcttaag ctctcttaca acaacatcta atgttaatcc
     7561 tgtatcaatt atgtcctcta caatcaatac atctttacct tctaaattac ctgtgaataa
     7621 ataatcaatc ttcacttgtc ctttagaatt agtgccgtca taactagaag ctcttaaaaa
     7681 ctccatttct aaatctaaat ttatatgtgt actaagctcg gttgcaaaag gaacagaacc
     7741 ttttaataga cagattaaaa caagcctttt accagcgtaa tctttcgtaa cttcttctcc
     7801 taattctttg caacggttct taatttcttc attagtaagt aatacttctt caactatctt
     7861 tttcattaat cctccttata agtattgaat aatcacaagt tttcgtaaga ctactattaa
     7921 ctaaaccaac gatagataaa acattgctat ctttatcttc taagattaag atatcttccc
     7981 tttctaaaat cccaatcttt tggtcaatta aataatcttt tacttttttg attccacttt
     8041 ttaatttaat tttgtctccg tcttttcttg tccttaactt aagcggaaac atatccatat
     8101 tataacatat attaaaatat ttgctatcaa taagattatc tttctttgat tttacttcta
     8161 acttattatc ttcataataa aagttttcct tatcaataat aagttcaaaa tctaattcct
     8221 ttttcaaata gggaacgata gttattttat catattcttt ataaaaagta aaactgtttt
     8281 taaaatatgt cttgatatta gccttatcag aactaattaa actaataatc tctaagacat
     8341 tttctttact taaattaaac ttttttaata gtttaaaaat aatttcttct tgaatataag
     8401 aatctaaagc taaaaaatct tctcttgcaa aacttaaatt agataccttc ttttcaataa
     8461 attcatcaac aatcttagaa acaattctcc aagctgcttt taaagtttcg ccatattctt
     8521 ctagcttata aacaccttgt tcattaactt ttaaaatttc cggatcaatt aaacttctaa
     8581 ttttgtttct agtataaata gtatctatat tacttttatc ttcaaaatac ttaacatttt
     8641 ctcttaaagc ataacgatag atatcttctt tttctaaatt gataaaaggt ctaactaaag
     8701 tatagcctgg atactttaca acactttcaa tcccagcata accagccaag ttactaccac
     8761 gtaaaatacg cattaaaatc gtctccaaat tatcgttagc atggtgagct aaaactaaat
     8821 aatcaatttt ttctttttta ataacttttt caaaaaattg atatcttaat ttccttgctt
     8881 ccttttgaaa gttttctgac tcatctaaat aatccaatct ttcaactgct aactttattt
     8941 cattttcctt acaaaaagac ttaatatact cttcttcaag attagcttct tttcttttca
     9001 agtgattgac aaaagctaca tatagatcta acttatattc ttttcttaaa cgcaaaaaac
     9061 tagcaagcat aaccattgag tcaactccag ttgatgttgc tattaaaact tttttgtttt
     9121 taagttctaa ctttttttct tcaaaaaaag cggaaataac tttatccata ctcgctcctt
     9181 tattaatata ttatataata attagccttt tcttgcaagg agtataatca aaaagagtgt
     9241 agcgctaaaa gctacactct ttaaacgatc tattctactt ttattcttct gcttcttcta
     9301 aagcaggaaa aacttttaac tctaaaagct tatcattaaa atctttcaaa tctttatact
     9361 ttttaataat aagatttctt cgatatgcat cataattaac aacgtcatca caatcaaaat
     9421 attctcccga ttgactagaa ataatcatat caccgatata aatattgtta tttcttgggc
     9481 aataagcaaa gaatggttga ttagataaac catctattcc aaaataatat tcttgtttta
     9541 aattaaatag attggaaatt gttctagaaa ctacagttgt gttgcgaact ttatcttgaa
     9601 ttactggggc ctcagtacga ttatgatcat agattacaaa aggaactctt gaaagtaaca
     9661 ttctctgaac aaaacgtctt tctaaatctt tccttcccaa ttctatctca tacattgtcc
     9721 taaatttatt tctaaataag tttgcattag gcaccattgt atctaagaaa tcaatagagt
     9781 ttccgtgatc tccgtaaaaa gcaataatcg tattatctaa tactcctaat gtttctaact
     9841 ctgctaagaa ctgaccaaaa caaaaatcca tataactaat atgttctaaa taacctagta
     9901 gcggatattc tattgatgaa taatcagcac taaagaaacg atccttttct aaaaataaat
     9961 taagatcctc ttcatatggc atatgcgtag aaattgtaat tggcattcca aaaaacttct
    10021 ttccttgatc gtttatggtc ttcacatcac tagccacata tctaagcaaa tctaaatcac
    10081 ttatccaacg atgaactatt ctattttcat cataaacacc taattctttt aagtcttctt
    10141 ccgatatatg ttcatcaaaa ttataactat tcacatgaac atttttacga tcataataat
    10201 agcctacatt agcatggtat gatttagtta catagtcttc attcttaaaa gattctgcta
    10261 gtgttggtaa atcaacacca acatgattaa aaacagtata tttatttcct gttggatata
    10321 aaccagttaa gacagtaaac tcagaatcag aagtattacc aataccagct gctgtataaa
    10381 agttattaaa gtaaattgat ttttcaagca tcttattaat gttaggcgtt agctctttat
    10441 aatcgccatc aactaaaacc tctaacccaa ttacaaattg atttaacgat tcagcttgaa
    10501 tcaaaattaa attcttatct tcataaatgc ctttatactc taagttagga ccataatttt
    10561 gtccatccaa aatatttatt tgatttacgt attggtgttg ttctaattta atttttagtt
    10621 cagctatctt ttcttctata agatcatcct tttctggtat tttctcgcta aaagcataat
    10681 caaaagcttc gaaaacataa taattaaata ctcccattga ctcagctgca tataaagctg
    10741 atgcattatc ttcataccaa gttcctttat ttacttcctt ataacttaca taactacttc
    10801 ccataaagaa gattcctaag aaggcaataa cgaaacctcc taaccatctt ctaaatccag
    10861 gaaatgaaga tgaaaaagtc ttttcttctt ctttacgaat aaacttataa tagtaaagga
    10921 atgttagaat aatgattgga ataaagaaaa ttatttttcc tcctctaata attataaaga
    10981 ttgattgtaa tacaaacaca aatgattcac ttgcccccgt atcacgagta aataaatcta
    11041 aattccaaaa tgagaaaaac attccatagt tagaaataaa aacacttaaa gcaaagtata
    11101 acagagaaaa gaaagtagta aggacaatca tataacgata ccttgcatta tcgctcttaa
    11161 atattaaaat tcctaatcca taaaacactg caaaaaaggc cgcatctcca ataatactat
    11221 ttacaaccat aaatgaatca cgaagatagg cactaggtaa agcattaact tttgtccaac
    11281 ttgataaata tgaattgata atatttaaca caaaaaagac taaagaaaaa actatgaact
    11341 tatttcgata tatatgtttc tccatatttt tgctcctata tataattata tcaaaaatcg
    11401 tttaagatta aaagttttta atacaaaaag tagacttgaa ttcaagtcta cttatttaaa
    11461 acaatctcga tatacttcc
//
LOCUS       DAOSGS010000018        11144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_4933_length_11144_cov_4.977726, whole genome shotgun sequence.
ACCESSION   DAOSGS010000018 DAOSGS010000000
VERSION     DAOSGS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4933_length_11144_cov_4.977726"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLQ22_01525"
     CDS             <1..446
                     /locus_tag="PLQ22_01525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH58674.1"
                     /translation="YLAKNNPDLNLVFIGKINGRSIRHLYKYRNIYLLGEIEYPYLRC
                     YLAYFDVCIIPFIINDLIQNTDPVKFYEYISAGKPVVSNMLPELQKYKDICYLYKSPN
                     SFNRNIMKALKENDPKIVAKRKIIARNNSWDKRVIEILNCISSIE"
     gene            443..1720
                     /locus_tag="PLQ22_01530"
     CDS             443..1720
                     /locus_tag="PLQ22_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH58675.1"
                     /translation="MKLKKIIKAIAEKTHIMFFWNVYKIYKLNGSKKAYKIIKNKVFN
                     LFISNKKLKSNFLRKIESIIDNIEKDQKVIISLSFIDWDIPLFQRPQHLALQLANKGI
                     IYFYCTLQNSNLKYFIEKQAENLYVLDEFDIILEKVKNKDKYLHVLSTNYFKQDTERI
                     EKAISLGYKIIYEYIDEISEDITGEKIENFVFERHKKLLKDESNCIVIATADKLYQEV
                     LKYRQKNCKLITNGVNVNHFRDNHYLVPKNIRKITSQNKTIIGYYGALAKWIDYKLLS
                     EIAAKRNDWNIVLLGVIYDQEIYKSDIIRAKNVYILPPVHYNTIPTYGKCFDVCIIPF
                     KINDITKSTSPIKLFEYMALGKPIVTTPMPECKKYKSVYIADNRADDFIEKINLALKN
                     NNKRYQKLLEKEALENDWSSKADEIINIISRKL"
     gene            1717..4812
                     /locus_tag="PLQ22_01535"
     CDS             1717..4812
                     /locus_tag="PLQ22_01535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH58676.1"
                     /translation="MSPKISVITTVYNTEKYLVRCLDSLIHQSYKNIEIIVVNDCSKG
                     NTDEIINKYLRKYQNIKYIKHNVNKGLYHARLTGADSATGDFIAFVDSDDYVSQDFYR
                     SMIFKATNENSDITFSQAIEVNNGRNYIMNLQYYDDEKSIEGNCLDYFFDQEGHNYRY
                     YTVWNKIFRMDLWKKARPFYSKQIKHLIMTEDIAFTTVLFSYATKISTCKNAFYYYVR
                     NEGSSTVNTNISINSIKKKISDISTSFDFVENFLLKRRLYKRYSKKFLEWKKLFSRIY
                     YSKIINLSFTQKEKNTLLRQLKKHFSKNFQLTISKESDFYYYSIRTEYNHHYEDIQNM
                     IIDKRVKLVSFDIFDTLVWRPLLYPTDLFLLMNTYFKDLSGSNTLLDFQRIRVEAEKY
                     ARQKSINEEITLTDIYKELEISFNIPNHITQKLKQREIELEYKYTKRRESVYNIFQLA
                     KYLGKKIIITSDMYLPTKVIKQILKSNGYENYDKLYLSSETKVSKYTGSVFKKMIQDY
                     NLTPNEIVHLGDNYQSDFETPNKLKIQGVLFPKASDIFFNYFPHLNNTGNCGKIFIKR
                     LPEWRSNAESLNFLGIRSMIALIANKYFDNPFRSFNPNTNFNGDPYFLGYYALGMHLF
                     SVTKWLIEKSIERKHKQMVFLARDGYLPLQAWKILNKHYKCKTKTVYFPFSRKASINL
                     LIQKPIDLYTITEYINIDKYNGTKILKLIKNVISSYDKGINKKYLKLGIKVNKYFSSL
                     DEFNKFISATIKLLYSQEKVNQNQAIYSKYFDKFFVSNSSIFDVGYSCKPEAIFAKLS
                     RRKLQTYFININSDKGLYYSKEFGVTLDTFFDYKPSLTGYLRELFISEQSSSCVSYDF
                     ENNELIPVFEKTKFSYFERNIVHNIQEGSLNFIKDLLSYFNEELDLLYIQNYYASLAH
                     ELYIHSSTETDKQIFSPIIFEDSIAAGKYSITNKWNTDLLFGNQIDTNKLFTLDYSNV
                     NSTMNLPANYSYQPKISKLIYLLLFNRALLKEKIKNKIHSPLIRNTLKHTYKTLKKFF
                     N"
     gene            4875..5996
                     /gene="wecB"
                     /locus_tag="PLQ22_01540"
     CDS             4875..5996
                     /gene="wecB"
                     /locus_tag="PLQ22_01540"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOH58677.1"
                     /translation="MKKILMIFGTRPEAIKMAPLYKELQDYKKDFKTVIALTGQHKEM
                     LYQVLDFFKIKPDYDLELMKPNQTLFDITSDALRGLEKILNKENPDLIIVQGDTTTVL
                     AGALAGFYKKIKVAHLEAGLRSGDKLSPFPEEINRILTSHIADYHWAPTKKAKENLLK
                     EGVNPKNINIVKNTVIDALFLAVKLLKTEGDKKYLNFFKGIDFKKKIVLVTAHRRESF
                     GEPFENICNSIKDLAVKNPNVEYVYPVHLNPNVREVVFRILSNIPNIHLLDPLDYPYL
                     VWIMNKSYMVLTDSGGIQEEAPSLGKPVLVLRDVTERQEGVDAGTAILVGTNRKKIIS
                     HAQRLLDDPKYYAKIAKATNPYGDGKASQRIVKLLHKIK"
     gene            complement(6047..6553)
                     /locus_tag="PLQ22_01545"
     CDS             complement(6047..6553)
                     /locus_tag="PLQ22_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58678.1"
                     /translation="MKNKTSNIIKVFQSINNSLRNLYINRKKAEEYTEIIEQGKDTII
                     CSTKDINLQNIKKELQFILVKISFYPINIIIALIPFILIIVLSPNRYDFVTAFYYFFG
                     LIWLLNNLKPNTLALISLLTLIPVPILLSFDIEFVAEAFASAFYTFLTIALIGIIIRL
                     IKNQIDSK"
     gene            complement(6543..8798)
                     /locus_tag="PLQ22_01550"
     CDS             complement(6543..8798)
                     /locus_tag="PLQ22_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58679.1"
                     /translation="MWNAGDGVYLWDENQFGMQRYSTVASRVLGYIVAKLFDSIEARS
                     NFVFYFGIVASAILVFLCGYFLYKRFAKSSKNGFILSIFTGIVSSSLFIVNNFIWEQL
                     IYYPGNYFFNLIPCIFIFLIFLIRYQKEIRLFDLLLIAISTIFINHQFFFVIFFLELF
                     AFFIYEVFFTSKMYTNLEQAKDTSTTSLCTSSSRQRGSIPYVNSLARKSSSKLGTSFA
                     SHINQNKHQRIKVILNYAISVLLILMISAYWLVPFLSNIFIVSPNSFYSSDNWEAVFQ
                     GYRDTSRTSNLMNYSNYPGNISGKLNGWIIPYVYYALLALPYAFFIIYFSKLKRIDKK
                     DYFLFFLIIVYVIFFNLGQGPNSYLWGDIWMWLFRTQSWFGFFRSFNRFFVISILINA
                     LLIPLSFILIKVKREIKIILAVILFICFAVTNFPILFTPYLDGTIGKMEVPQEYFDFN
                     NKLQTDANDNYYVYILPALIYESYNWSIEGYNENYTSNYYFAELFYKKPILMEGGAYV
                     LSNYSDTFNKLFQIDLGTFIFYDNFDEMIDNAGIKYILIKKDLIDVTNENTPPDYQKY
                     ENYFIYNPNYTKIEENDYFNIYENNNYKEYISVKEKGNCDISYHRISPVEYTFDLKCN
                     TNKVSLEMLNTYDIRWEIIPNQNTSDIQNIIKNKSILSEQHTSGELSNNSWEIDLDQL
                     SSLSINNDDIDIILDQDGNYIFKGTFYYYPQVSYRIFSHVSVISLAFTILLTISIGGF
                     LLIKNKNKYEK"
     gene            complement(8847..9785)
                     /locus_tag="PLQ22_01555"
     CDS             complement(8847..9785)
                     /locus_tag="PLQ22_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH58680.1"
                     /translation="MKKLISIITPAFNEAENIDKYYAEVNKVIEKLKEKYDFEVLIIS
                     DGSTDQTVGNVEKLVKKDKRYHLIEFSRNFGKEIATSAGYMNCRGDAAIILDADLQFP
                     QEMIPQFIEKWEKGNDVVVGVRKNNSGNFISKMGSKLFYYILNRISDTKSVPGSTDFR
                     LIDRKVIDQLNKFTERNRITRGLIDWMGFNRDYILFEARERVGGRPSYNLIKKVRLAI
                     SSFVGLSLFPLKIAGYLGIFIMIVSTLMLVAIFIGRYILHNPYISSVTGTAILAIILM
                     FFVGVILSCLGLISLYIATIYNEVTNRPLYIIKRII"
     gene            complement(9954..11096)
                     /locus_tag="PLQ22_01560"
     CDS             complement(9954..11096)
                     /locus_tag="PLQ22_01560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH58681.1"
                     /translation="MKSDFVIVAHKFNPQPDDELIKYLVNKGQNVVQVCHSFSDADNR
                     KSWISQFFSDGKVIKRETKDYKNLPEVLIYVKEFFFTLSSLLKLKNRFKYYIAMDGLC
                     LVYGYLLKVFHKVDKTIFWAMDYVPENRFGSKIKNIIYSNVNKFGYKNADQMWDISPR
                     MAEAREKYAGIKRTQYKKHKIVPYGMWLSRIRQYEYKDCKKNTLVFMGHIIEKQGVQF
                     VLEAIPNIIKIIPDFRFTIIGGGEYLDTLKNIAEKKGILRYCNFLGRIEKLEDVEKEI
                     AKSTVAIAPYSKELDTWTYYADPGKVKTYLACGVPVLLTDIPWNAKEIAQVKAGEIIT
                     LDPNEMADAIVKLMDSKVNQQFRNNAIKYSKVFDYSMIFGELFKSM"
BASE COUNT         4196 a         1694 c         1582 g         3672 t
ORIGIN      
        1 aataccttgc taaaaataat cctgatttaa atttagtttt tattggcaaa atcaatggtc
       61 gatcaataag acatttatac aaatacagaa atatatattt attgggcgaa atcgaatatc
      121 cctatttacg ctgttatcta gcctattttg atgtttgtat aattccattt ataattaatg
      181 atctaattca gaacactgat cctgtaaagt tttacgaata tatttctgct ggcaaacctg
      241 tagtatcaaa tatgttacct gaattacaaa aatataaaga tatttgttac ttatacaaat
      301 cgccgaatag tttcaacaga aatatcatga aagctcttaa agaaaacgat cccaaaattg
      361 tcgcaaaacg aaaaataatt gcccgaaata atagttggga taagagagtt attgaaatac
      421 taaattgcat aagttccata gaatgaagct taaaaaaata atcaaagcta tagcagaaaa
      481 aactcatata atgttttttt ggaatgttta taaaatttat aaacttaatg gctcaaagaa
      541 ggcttacaag attataaaaa ataaagtatt caatttattc atttcaaata agaaattaaa
      601 gtcaaatttc cttagaaaaa tagagagtat tattgacaat atagaaaaag atcaaaaggt
      661 aataatatct ctatccttta ttgattggga tataccactc ttccaaagac ctcagcatct
      721 cgcattacag cttgcaaata aaggtattat atatttttat tgtacattac aaaatagtaa
      781 tctaaaatat ttcatagaaa agcaagcaga aaacctttac gttctagatg aattcgacat
      841 tattcttgaa aaagttaaaa ataaagataa atatttacat gttctttcta caaattattt
      901 caagcaagac acggaaagaa tagaaaaagc tatttcactg ggttataaga taatatatga
      961 atatatcgat gaaatttccg aggatatcac aggtgagaag atagagaatt ttgtatttga
     1021 aaggcataaa aaattattaa aggatgaatc aaattgtatt gtaattgcaa cagcagataa
     1081 gctttaccag gaagtattaa aatatcggca aaagaattgc aaactaatca caaatggagt
     1141 aaatgtcaac catttcagag ataatcatta tctcgttcct aaaaacatca gaaagattac
     1201 atctcaaaat aaaacaataa ttggatatta tggtgcactt gcaaaatgga tagactacaa
     1261 acttttgtct gagatagcag ccaaaagaaa tgattggaat atagtattat tgggagtaat
     1321 ttatgatcaa gaaatataca aatcagatat aataagagct aaaaatgttt acatattacc
     1381 cccagtacat tataatacga taccaaccta tggaaaatgc tttgatgtgt gtattattcc
     1441 ttttaagata aatgatatta caaaatcaac atctccaatc aagctttttg aatatatggc
     1501 tttgggtaaa ccaatagtaa caacgcctat gccagaatgc aaaaaatata aatctgtata
     1561 tattgcagat aatagagccg atgattttat tgaaaagatt aacttggctt taaaaaataa
     1621 caacaagaga tatcaaaagc ttcttgaaaa ggaggctctt gagaacgatt ggagctcaaa
     1681 agcggatgag ataattaata taattagtcg taagttatga gtcccaaaat atctgtaatt
     1741 accacagtct ataatacgga aaaatactta gtaagatgcc tcgattcatt aattcatcag
     1801 tcatataaaa atattgagat tattgtagtc aatgattgct ctaaaggaaa tactgatgag
     1861 attatcaata aatatttacg taaatatcaa aacatcaagt acataaaaca taatgtaaat
     1921 aagggtttat accacgctcg tttaactggt gctgatagtg ctacaggtga ctttattgcc
     1981 tttgttgaca gtgatgatta tgtttctcaa gacttttatc gatcaatgat attcaaagca
     2041 acaaatgaaa attcagatat tactttctca caagcaattg aagtaaataa cgggagaaat
     2101 tacatcatga atcttcaata ttatgatgat gagaaatcta ttgaaggaaa ttgtctagat
     2161 tatttttttg accaagaggg tcataattat cgatattaca cagtgtggaa caagatattt
     2221 cgaatggatc tctggaaaaa agctaggcct ttttattcca agcaaattaa gcatttaata
     2281 atgactgaag acatagcttt tacaactgtt ctgttctcat atgcaacaaa aatctctacc
     2341 tgtaagaatg cattttatta ttatgtaaga aatgaaggct cgtcaacagt aaatacgaat
     2401 atttctataa attcaattaa aaaaaagata tcagacatct caacaagttt tgattttgta
     2461 gagaattttc tattaaaaag acgactatat aaaagatatt ccaagaagtt tctagaatgg
     2521 aaaaaattat tctcacgcat atattactcg aaaataatca acttaagttt tactcaaaag
     2581 gagaagaaca cattacttcg gcagcttaaa aagcacttta gtaaaaattt tcaactaact
     2641 ataagtaagg aatctgattt ctattattac tcgattcgaa ctgaatacaa tcatcattac
     2701 gaagacattc aaaacatgat tattgacaaa agagttaaat tggttagctt cgatattttt
     2761 gatactttag tatggagacc cctgttgtat ccaacggatc tattcttact aatgaacaca
     2821 tattttaaag atctatcggg ttcaaacact ttacttgatt tccagaggat acgtgttgaa
     2881 gctgagaaat atgctcgcca aaaaagtatt aatgaggaaa ttacacttac agatatctat
     2941 aaagaacttg aaataagctt taatatccca aatcatatta cccaaaagct taaacagagg
     3001 gaaatcgaat tagaatacaa atatactaaa agaagagaaa gtgtttacaa catatttcaa
     3061 cttgcaaaat atctcgggaa aaagataata attacttccg atatgtatct ccctacaaaa
     3121 gtaataaagc aaattcttaa aagtaatggc tatgaaaatt atgataagct atatctatca
     3181 tcggaaacta aagtatccaa atatactgga tctgttttca aaaaaatgat tcaagattac
     3241 aatcttacgc caaatgaaat tgtgcattta ggagataact atcaaagtga ttttgaaaca
     3301 ccaaataagc ttaagattca aggagtatta ttccctaaag cctccgatat ttttttcaat
     3361 tattttcctc atttaaataa tactggtaac tgtggaaaaa tttttattaa aagattgcct
     3421 gaatggcgta gcaatgccga atctctaaac tttcttggca taagatcaat gattgcacta
     3481 atcgcaaaca aatattttga taatccattt agatccttca atccgaacac aaatttcaat
     3541 ggggatccat atttccttgg atactacgct ctgggaatgc acttattttc tgtaacaaaa
     3601 tggttaatcg agaagagtat tgagcgaaag cataaacaaa tggtttttct ggctagagat
     3661 ggatatctac ctctacaagc atggaaaata ctcaataagc attataaatg taaaacaaaa
     3721 actgtatatt tccccttttc acgaaaagca tctatcaatt tgctgataca aaaaccaatt
     3781 gatttatata ctataaccga atacatcaat attgataaat acaatggtac taaaattctt
     3841 aaactaatta aaaatgttat ttctagctat gataaaggaa taaataaaaa atatctaaag
     3901 ctcggtatta aagtaaataa atatttttct tccttggatg aatttaacaa atttatttca
     3961 gcaaccataa aacttttata tagtcaagaa aaggtcaatc agaatcaagc aatatatagt
     4021 aaatactttg ataaattctt tgttagtaat tcaagtattt tcgatgttgg atatagctgt
     4081 aagccagaag cgatttttgc aaaactaagt agaagaaaat tgcaaacata ctttattaac
     4141 ataaatagtg ataagggact atactattca aaagaattcg gagtaacttt agacacattt
     4201 ttcgattata agccatcact aaccggatat ctgagagaac tttttatttc cgaacaatca
     4261 agctcatgtg tgtcatacga ctttgaaaac aatgaattaa tacctgtttt cgagaaaacg
     4321 aaatttagtt atttcgaaag aaatattgtt cataatatcc aagaaggttc attgaacttc
     4381 ataaaagatc tactaagcta ctttaatgaa gagcttgatc ttctatacat acaaaactat
     4441 tatgcgtccc ttgctcacga actatatatt cattcctcta ctgaaacaga taaacaaata
     4501 tttagcccaa taatatttga agattctatc gcagctggaa aatatagtat tacgaataag
     4561 tggaacaccg acttactctt tggaaatcaa attgatacta ataaattatt tacattagac
     4621 tattcaaacg taaatagcac aatgaatctt ccagcaaatt attcatatca gcccaaaata
     4681 tcaaagctta tatacttatt attgtttaac agagcactat taaaggaaaa aataaagaat
     4741 aaaatacata gtcctttaat tcgcaacact ttgaagcata catacaaaac attaaaaaaa
     4801 ttttttaatt gattagagaa aaaataagtt ttttaggtgg tgaaaactat attactttgg
     4861 ttattctctc aaatatgaag aaaatcttaa tgatatttgg aacaaggcct gaagcaatca
     4921 aaatggcacc attgtataaa gagcttcagg actacaaaaa agatttcaaa acagtaattg
     4981 ctctaacggg acagcacaaa gaaatgctat atcaggtctt agattttttt aagattaaac
     5041 cagattacga tttagaattg atgaaaccta atcagacatt atttgatatt acctcagacg
     5101 ccttaagagg acttgaaaaa atactaaata aggaaaatcc cgatttgatt attgttcaag
     5161 gtgacacaac aactgtacta gctggtgctt tggccggatt ctataagaaa attaaggttg
     5221 cacatctcga agcaggatta cgaagtggtg ataaattatc cccatttcct gaggaaataa
     5281 atcgtattct cacaagtcat atcgctgatt atcattgggc accgacaaaa aaagcaaaag
     5341 aaaatctact taaagaaggg gtaaacccaa aaaatattaa cattgtaaaa aacacagtaa
     5401 ttgatgcact ttttcttgca gtaaaactat tgaaaaccga aggtgataaa aaatatttga
     5461 acttttttaa gggaatagat tttaaaaaga aaattgtctt agtaactgct cacagaagag
     5521 agagttttgg agaaccattt gaaaacatat gcaattcaat taaagattta gctgttaaaa
     5581 atccaaatgt tgagtatgtc tacccagttc atttaaatcc aaatgtgaga gaagtcgtat
     5641 ttagaatatt aagtaatata cctaatattc acctactcga tcctttggat tatccatatt
     5701 tagtatggat tatgaataaa tcctatatgg tacttactga ttctggtggg atacaagaag
     5761 aggctccttc actaggtaaa cctgtattag ttttaagaga tgttacagaa cggcaagaag
     5821 gcgtagatgc cggaacagcg atattagttg gaactaatcg caagaaaatc atttctcatg
     5881 ctcaacgatt attagatgat cctaaatatt atgcaaagat agctaaagca acaaatccgt
     5941 atggagatgg taaagcttca cagcgaattg taaaactttt acacaaaatc aaataaagag
     6001 taacggtatt gagtaaaaaa ctttaatctt aagtctgtca gtaatgttat tttgaatcga
     6061 tttgattttt gataagcctg attattattc ctattaaagc aattgttaaa aaagtataaa
     6121 aagcggatgc aaatgcttca gcaacaaatt cgatatcaaa tgaaagtagt attggtactg
     6181 gaattaatgt cagtaaagaa atcagtgcta aagtatttgg tttcaaattg tttaaaagcc
     6241 atatcaaccc gaaaaagtaa tagaatgcag ttacaaaatc atatctgttg ggagatagaa
     6301 ctattattaa tataaaagga attaaggcaa taattatatt tatagggtaa aaagatattt
     6361 tcactagaat aaactgtaat tctttcttta tattttgaag attgatatcc ttcgtagaac
     6421 atattatagt atctttgcct tgttctataa tctcggtata ttcttctgct tttttacgat
     6481 taatatagag gtttcgtaaa ctattattta tagattgaaa tacttttatg atatttgatg
     6541 ttttattttt catacttatt tttattcttg atcaatagaa atccgcctat gcttattgtt
     6601 aagagtattg taaatgctag tgagattacc gaaacatgag aaaatattct atatgatact
     6661 tgaggataat aatagaatgt gcctttgaaa atataatttc catcctgatc caaaataata
     6721 tcaatgtcgt cattattaat agagagagat gataattgat cgagatcaat ctcccaacta
     6781 ttatttgata actctccaga agtatgttgt tctgacagga tactcttatt tttaattata
     6841 ttttgtatat cacttgtatt ttggttaggt attatttccc atctgatatc gtaagtattt
     6901 agcatttcta atgaaacctt atttgtgttg cacttcaaat caaaagtata ttcgactggt
     6961 gatatcctat gataagaaat atcacaattt cccttctctt ttacagaaat atactctttg
     7021 taattattat tctcgtagat attaaagtag tcattttctt caatctttgt gtagttagga
     7081 ttgtaaataa aatagttctc atatttttga tagtcaggag gggtgttttc gtttgtaaca
     7141 tcaataagat ctttttttat aaggatatat ttgataccag cattatctat catctcatcg
     7201 aaattatcat aaaaaataaa tgtaccaaga tctatttgaa ataatttatt gaaagtatca
     7261 ctataattac tcaatacata agctccacct tccatcaaaa taggcttttt ataaaataat
     7321 tctgcaaaat aataattgct agtataattt tcgttataac cttcaattga ccaattatat
     7381 gattcataaa tcagagctgg aaggatatat acataataat tatcgttagc atctgtttga
     7441 agtttattgt taaaatcaaa atattcttga ggtacttcca tcttaccaat agtcccatct
     7501 aaatatggcg taaagagtat cgggaaattg gttactgcaa aacagataaa tagtatcact
     7561 gctaaaatga ttttaatctc ccttttcacc ttaattaaaa tgaatgaaag aggtattaat
     7621 aaagcattaa tcagaattga tattacgaaa aatctgttaa aggatctaaa gaatccaaac
     7681 catgattgcg tccgaaatag ccacatccaa atatctcccc ataaatatga attcggtcct
     7741 tggccaaggt taaaaaatat tacataaaca attatcagga aaaacagaaa ataatccttt
     7801 ttatctattc ttttaagctt actgaaatat atgataaaaa aagcataagg taatgctaac
     7861 aatgcataat aaacataagg gattatccag ccattgagct taccagaaat atttccggga
     7921 tagttgctat aattcataag attagaagtg cggctagtat ctcgatatcc ttgaaaaact
     7981 gcttcccaat tatctgacga atagaaagaa tttggagaaa caataaagat gttactcaag
     8041 aaaggtacta gccagtaagc agaaatcata aggattaata gaacagaaat agcataatta
     8101 agtattacct ttatcctttg atgtttattt tgattaatat gtgaagcgaa agaagtacca
     8161 agtttgcttg aggacttccg agcaagtgag ttaacataag gtatactacc tcgttgcctg
     8221 ctactagaag tacacaacga agtagtggaa gtatccttag cttgctctag attagtatac
     8281 attttactag taaaaaacac ttcatagata aaaaaagcaa ataattcgag gaaaaatatt
     8341 acaaaaaaga actgatgatt aataaatatt gttgaaattg caattaataa taggtcaaat
     8401 aatctaatct ctttctgata tctaatcaaa aatattagaa aaataaaaat acatggtatc
     8461 aagttaaaaa agtaattccc aggataatat attagctgct cccagatgaa attattaaca
     8521 ataaatagtg agctactaac aatacccgta aatattgaga ggatgaaacc attttttgat
     8581 gatttagcaa agcgtttata caaaaaatac ccacaaagga atactaatat tgctgaagca
     8641 acgataccaa aataaaatac aaagttagat cttgcttcta tactgtcaaa aagttttgca
     8701 acaatatatc caagtactct agaggcaaca gtagagtatc tttgcatacc aaattgattt
     8761 tcatcccaaa gataaacgcc atctccagca ttccacatta ttggggaatc tccgtaacca
     8821 aagtacataa agcatagaat aaatacttat ataatccttt taataatata taaaggacga
     8881 tttgttactt cattatagat tgttgcaata taaagagata tcaaaccaag acatgatagt
     8941 atcacaccta caaaaaacat caagatgatt gcaagtattg cggtacctgt aacacttgag
     9001 atatatggat tgtgcaatat atatctacca ataaatattg caactagcat tagtgttgaa
     9061 actatcataa taaatatgcc tagatatccc gcaattttta atggaaaaag gcttaggcca
     9121 acgaaactcg aaattgctaa acgaactttt ttgattaagt tataagatgg cctaccacct
     9181 actctttcgc gtgcttcaaa aaggatataa tctcgattaa aacccatcca atcgatcaaa
     9241 cctcgtgtta tacgatttct ttcagtaaat ttatttaatt gatcaattac tttacgatcg
     9301 attaacctaa aatcagttga acccggaaca cttttagtat cagaaattct atttaatata
     9361 taataaaaaa gcttagagcc cattttggaa ataaaatttc cactgttatt tttccttacg
     9421 cctactacaa catcattacc tttttcccat ttttcgatga attggggtat catttcttga
     9481 ggaaattgaa gatcagcatc aagtattatt gccgcatccc ctctacaatt catatatcca
     9541 gccgatgtag caatttcttt accaaaattt ctagaaaatt ctattaggtg atatctttta
     9601 tcttttttta ctagcttttc gacattacct actgtttgat ctgtactccc atcgctaatt
     9661 attaatactt caaaatcata tttctctttt aatttttcga ttactttatt tacttcagca
     9721 taatacttat cgatattttc tgcttcatta aatgctggag tgattattga aataagtttt
     9781 ttcatataat tactaggatt ttacaattaa tcgattattt gtcgatactt ctatatacaa
     9841 cttgcgcaaa aaatatactt caagctaagt aaaatatttt atttatctat actcaattat
     9901 atcatttata tacataaaaa tcattatgta ttacctttgc cttgagttat gcattacata
     9961 ctcttaaata attctccaaa aatcattgaa taatcaaaaa ccttgctata ctttattgcg
    10021 ttatttctaa attgttgatt gacttttgaa tccatcagtt taacaattgc gtctgccatc
    10081 tcattgggat ctaaagttat tatctcccct gcttttactt gagcaatttc ttttgcattc
    10141 cagggaatat cggtaagcaa tacaggtact ccacaagcaa gatatgtttt tacttttcct
    10201 ggatcagcat aatatgtcca agtatctaac tccttggaat aaggtgcaat agcgaccgtt
    10261 gattttgcaa tctctttctc cacatcttca agtttttcaa tcctaccaag aaaattgcaa
    10321 tatcttaata ttcctttttt ttctgcaata tttttaagcg tatcaaggta ttctccacct
    10381 ccaataattg tgaatctgaa atctgggatt atttttatta tgtttggtat agcttcgaga
    10441 acaaattgta caccttgttt ctcaataata tgccccataa aaactaatgt gtttttttta
    10501 caatctttat attcatactg tctaatacga gataaccaca tgccataagg aacaatctta
    10561 tgctttttat attgagtcct tttaatccca gcatattttt ctcgagcttc tgccattcgt
    10621 ggggaaatat cccacatctg atctgcattt ttatatccga acttattaac atttgaatat
    10681 attatattct ttattttact accgaaacga ttctcaggaa catagtccat tgcccagaaa
    10741 atagttttat ctaccttatg aaatactttt aataagtagc cgtatacgag acacaagccg
    10801 tccatagcaa tataatattt gaagcgattc tttagcttta acaatgagct tagtgtaaaa
    10861 aaaaactctt taacatagat cagaacttca ggaagattct tataatcctt cgtctcgcgt
    10921 ttgattacct taccatctga aaaaaactga gatatccagc tttttcgatt atccgcatct
    10981 gaaaagctat ggcagacttg gacaacattc tgacctttat taactagata ttttatcaac
    11041 tcatcgtcag gctgtgggtt gaacttatga gcaactataa caaaatctga tttcattttt
    11101 atttttttgg attaaatttt tttaaaatct ttatacaatt taat
//
LOCUS       DAOSGS010000019        10915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_5056_length_10915_cov_4.321547, whole genome shotgun sequence.
ACCESSION   DAOSGS010000019 DAOSGS010000000
VERSION     DAOSGS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5056_length_10915_cov_4.321547"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..295)
                     /locus_tag="PLQ22_01565"
     CDS             complement(59..295)
                     /locus_tag="PLQ22_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58682.1"
                     /translation="MDWVQITMIIISGAILLSLLIALIKGIYLSKRRFICSECNQEFY
                     PKWYQVMFELHHFEYFRIRCPHCGKKKFHRCIDE"
     gene            complement(319..849)
                     /locus_tag="PLQ22_01570"
     CDS             complement(319..849)
                     /locus_tag="PLQ22_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH58683.1"
                     /translation="MDCIKDIEFRKPNFNDINEIESFKQEFIDTNDSMDGTGKLSNLS
                     AEEWLRLNQKMEHESTSELVRNLQYGLFRGKRLLGLIDIRLELRGYLVEFGGHIGYCV
                     RPLERRKGYGKLMLQKALEVCKEINLNKVLITCLEDNIASAKIIEACGGVYEKTVFDD
                     KNYQERLKRYWIDLDK"
     gene            complement(952..1028)
                     /locus_tag="PLQ22_01575"
     tRNA            complement(952..1028)
                     /locus_tag="PLQ22_01575"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(992..994),aa:Lys,seq:ctt)
     gene            complement(1089..1664)
                     /gene="pduL"
                     /locus_tag="PLQ22_01580"
     CDS             complement(1089..1664)
                     /gene="pduL"
                     /locus_tag="PLQ22_01580"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOH58684.1"
                     /translation="MKNKILIETSARHIHITKEDYEALFGKGAQLTFKKELSQPGQFQ
                     AEERVTIVGPKRELERVSILGPFRKETQVELSATDARSIGVDSVVRLSGDIKGTPGCK
                     IVGPKGAIDIKEGVIVAKRHIHLTEVKAKELGLKQGDVVKVEVKNDTRSLVFGDVEIR
                     VSSTYDNAMHIDTDESNAGSVAFGTLGTIIK"
     gene            complement(1675..4254)
                     /locus_tag="PLQ22_01585"
     CDS             complement(1675..4254)
                     /locus_tag="PLQ22_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58685.1"
                     /translation="MRKVIKIIFATIMGIVLCIGLSILTFYLLLTAGTANPPEELYVA
                     QNQEFSFEDILQKSLMNTKEDQKVKLTLTEDELNKLIYSLIVQEINPDYYKTEEDRAI
                     YDSPEVKIRSVYGEIKDKYLYIRAHVDSYIDVLVTLKLEIKQTETDFKLVVKKLQLGK
                     FNLTSGLGRLAIEKILLPQIDLEAELNNNFEESDIPVKFTWEDLSFTLTKADIKTKVN
                     SMLAESMDNPSYGTIISELLDTFIDTKGVINLGISENDGIGLVLDVTEFAYDGEITAP
                     RFDFEVFKTKILTLDENGVINNDNSGLIISYLLVGYANISEENRAIINACDFSSIGIA
                     DKTAHLGYANISTSDENIEDDIINQLYSEVGDLSDGLIQIGLDEATINNILAATPIIG
                     EGFSLYRSNSAYEKYTFVGVESLFCDVVNDGLSFSLALSLNGQRLLLSTSLEQDTLVT
                     TDVKLELTDISLGNYHMSAEAKEAILGILGEVVGDNELILIQGNTISIMTSDLTSFLN
                     TEEGVIDTIIDETLPEILTFELVGVEGITGGALNVKLNLVDFKTTQTLPDEVKEPFVQ
                     ESFVTEQALRILFSDTAGTSEIRFTDLDINRKVYQDTHQYADLASSELLPDGETTLNI
                     TVEGIIFHFYSPITTVEYLVKINGLKTRAYLELTISNNDSPEVILTPSPTLLIGTKNI
                     DSQFLLNYLSASFTNNDVLKYDEAQGVFKLSANSFYELMTVGGSSAFTVDKIKIHNGG
                     INITVSVSEDQLALLAALAAAQAAFDEITQQDFFDPSLYNTLDPEQAAAIEEMQNQLE
                     EIKNNQLNGEPISEEDINALLDNVNGLSEENKGIFFDQVSGEFDNQGYAGLLEALELA
                     MYL"
     gene            complement(4251..4784)
                     /locus_tag="PLQ22_01590"
     CDS             complement(4251..4784)
                     /locus_tag="PLQ22_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58686.1"
                     /translation="MLVIVGPSASGKTEISRYLIKEYNLKRVITCTTRPMRWDEKDGR
                     DYYFLTEEEFKAGIEDNLFIEYTKYNNDYYGTYYKEIADDKIIILDQVGFLNIKKILK
                     DSIVSFYIETPLAERKMRMVKRGDTIKRIYQKIEYDNQNFPSKIPGIDYYITNFADSI
                     SKSAKKIYNIYKERIGL"
     gene            complement(4795..6222)
                     /locus_tag="PLQ22_01595"
     CDS             complement(4795..6222)
                     /locus_tag="PLQ22_01595"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOH58687.1"
                     /translation="MNYTMMTDFYELTMANGYFLSGKKDEIVYFDLYYRDVPDRGGFA
                     IFAGLKDIVEYIQNLKFTKKDIDFLRSKNIFSEEFLKYLKDFKFSGDIYAMPEGSVFF
                     PGEPVMTVRAKAIEAQLIETYVLAVFNHQSLIATKANRVVRAAKGRVVSEFGARRAHG
                     AEAAYKGARAAYIGLVNSTSNTLADVLFGIPASGTMAHSWVQMFDTELEAFETYCKMY
                     PNNSLLLIDTYDTLKQGIYNAIKAFDNILKPLGKRPLGVRIDSGDLAYQSKQLRKILD
                     KAGYSDCKIVVSNSLDEYSITALLEQGAKIDIFGVGERLITSKSWPVFGGVYKLAAIE
                     QDGVIIPKIKISESVDKITTPHFKQVYRLYENGKASADYITIHDEVVDDSKPLEIFDP
                     YATWKRKTLETFEAKALLIPIFKKGKLVYKMPDLLEIREHVRKEIDSLWEEVKRFDQP
                     HNYYVDLSQKLWDCKEELLLKERKN"
     gene            complement(6309..6863)
                     /gene="mscL"
                     /locus_tag="PLQ22_01600"
                     /pseudo
     CDS             complement(6309..6863)
                     /gene="mscL"
                     /locus_tag="PLQ22_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
     gene            complement(6917..7294)
                     /locus_tag="PLQ22_01605"
     CDS             complement(6917..7294)
                     /locus_tag="PLQ22_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOH58688.1"
                     /translation="MNKKIFEAGEMYLETLYVLKLESEEVRSVDIVNYTGKAKSSVSQ
                     ALNTLKDDGFIIIDENGYLDFTEKGKKIAFKIYDRHTTLTKVFISLGVSEEQAEEDAC
                     KFEHDISDETFQAIKKHLEKHKN"
     gene            complement(7287..7475)
                     /locus_tag="PLQ22_01610"
     CDS             complement(7287..7475)
                     /locus_tag="PLQ22_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOH58689.1"
                     /translation="MTLIEILVIVVAVIFVVLVVVNSFRKKKKGECSSCSSCTKECAL
                     RKTNLDELKEQLRKGIYE"
     gene            complement(7472..9724)
                     /locus_tag="PLQ22_01615"
     CDS             complement(7472..9724)
                     /locus_tag="PLQ22_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOH58690.1"
                     /translation="MLNIGIVGNPNSGKTTLFNNITGSTAHVGNWPGVTVDKKEGIYK
                     RGKEKILVTDLPGNYSLSPYTDEEVITRNFILHSKPDCIINIVDVTNLERNLYLTTQI
                     MELGVPMVLAVNMMDLAERENIKISIPNLEKDLGIRAVEICALKGSGTKELMEVAIEE
                     AGKKREPKSLLKLTKLKGLIDLVESKLEEIGISSPLFHAIKLIEGDEIEVNDHPKLYE
                     EIQEKSKEYQSATFKDDNEGMIADARYQAIEKISKDIFIQKKEKLDIIKQSSKRDKIF
                     THPVFGIPIFLIIMFLVFHVTFSENFLFLGGLFGDDFVTLSGTSFEGLFGEGGINSPG
                     AMLLTLIETLGDLFMGWFAGVLEGAGAASWLVGFLVDGVFAGLFAVLSFLPQILILYF
                     FLSILEDTGYMARIVFLLDRLFKKMGLSGRALIPMVMGFGCSIPAIMNTRTLNSREEK
                     LATIRVIPFFSCGAKLPILTAIAGAIVMHFGIGNADLITFGMYILGIVTAFIAIVIMS
                     KTTLKGMDSPFIMELPPYRSIKFSSLMIHLWDKMKHFIQKAFTVILASTIVIWAISHF
                     SFGWRFLEDSQLNESILGKFGQLVQPLFTPLGFGSQLNQYGWVFAVAIVTGLIAKENV
                     VATFGTLAAAIIAGFSGDEEGVSSVIQIIGATGITVPALISFIAFNMLTIPCFAAVAS
                     AKGELTRKDFNWTILFWFVTSYLVSTIVYVIGTWWWAIFIVLAILASVITIIEINYRK
                     YQKRKVEVTA"
     gene            complement(9717..9977)
                     /locus_tag="PLQ22_01620"
     CDS             complement(9717..9977)
                     /locus_tag="PLQ22_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOH58691.1"
                     /translation="MKKHNKKLSDFKINETGIVIAVLGKGQIRRRLLDMGITNDVKIS
                     LIKKSPFGDPLEVFLRGYVLTLRKSEASLVEMKMLDEEGNDA"
     gene            complement(10077..>10915)
                     /locus_tag="PLQ22_01625"
     CDS             complement(10077..>10915)
                     /locus_tag="PLQ22_01625"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH58692.1"
                     /translation="VMGSAMLLNLKKKGFEISYYARNYEKTKDKLQGVKYYPSIKDLT
                     LANDVIITIVGYPSDLEEVYYKEGNIIDNLGKGKIVIDMTTSSPKLAKRIYQDVKKKS
                     GFSLDAPVTGGDKGAIEGTLVIFVGGDKKAYEKCLPVFKGMGTTILYTGEAGSGQHSK
                     LANQISIAGAISGMAETISYAKKAGLDVAPLIEVWSKGSAGSWQMKNLGPKALANDFR
                     PGFYIKHFIKDMNLAIDEAKENNLDLKMLTTVRDMFEELSKLGYDECGTQALIDYYNR
                     KS"
BASE COUNT         3600 a         2104 c         1222 g         3989 t
ORIGIN      
        1 atttttctta aaaaggcatt aaaaagagtt aggatttacc taactctcta tttccatatt
       61 attcatctat acatctatgg aatttcttct tcccgcaatg cggacatcta attctaaaat
      121 attcaaaatg atgcagttca aacataacct gataccattt aggataaaac tcttgattac
      181 attctgagca tataaatctt ctttttgata aatagattcc tttaattaaa gcaattagta
      241 aacttagtaa aattgctcct gaaattatta tcatagtaat ttgaacccaa tccattttta
      301 tcctccttaa aattaaattt atttatccaa gtcaatccag tagcgtttta acctttcttg
      361 ataattttta tcatcaaaaa cagttttctc atacactccg ccacatgctt caattatttt
      421 agcgcttgca atattatcct ctaaacaagt tattaatact ttatttaaat tgatctcttt
      481 acacacttct aaagcttttt gaagcataag ttttccataa ccttttcttc gttcaagagg
      541 cctaacacaa taaccaatat gtccgccaaa ctcaactaaa tatcctctta attctaatct
      601 aatatcaatt aatccaagta atctcttacc acgaaatagt ccatactgta aatttctaac
      661 aagttcagaa gtactttcat gctccatctt ctgatttaat cgtagccatt cttcagcact
      721 aagattgctt aacttaccag taccatccat actatcatta gtatcaataa attcttgttt
      781 gaatgattct atttcattaa tatcattaaa gtttggtttt ctaaattcta tatcttttat
      841 acaatccatt ataaatctcc aataactctc taaatataag tatacattaa aactaaattt
      901 atttctataa aaaaagacca atattactat tggtctttta tataaacaaa atggtacacc
      961 ttatgggact cgaacccatg acccattgat taagagtcaa ttgctctggc caactgagct
     1021 aaaggtgcat ttttatcttt aagtattata cacctttttc ccataagttg caagtattgt
     1081 tatttaaatt attttataat cgtacccaat gtaccaaaag ctacactacc tgcatttgac
     1141 tcatctgtat caatatgcat agcattgtca taagttgaac ttactctaat ctctacatca
     1201 ccaaacacta atgatctagt atcattttta acttctactt taactacatc gccttgtttt
     1261 aaacctaatt ctttagcttt tacttcagtt aaatgaatat gtcttttagc tacaataacc
     1321 ccttctttaa tatcaatagc tcctttggga cctacgatct tacatccagg tgttccttta
     1381 atatctccac ttaatctaac tacactatca acaccaattg atcttgcatc tgtagctgat
     1441 aattctactt gtgtttcttt tctaaaaggt cctaaaatag aaactctttc taattctctt
     1501 ttggggccta caattgtaac tctttcttct gcttggaatt ggccaggttg agataattct
     1561 tttttaaaag ttaactgtgc accctttcca aataatgctt cataatcttc tttagtaata
     1621 tgaatatgtc tagctgatgt ttcaattaaa atcttattct tcatgttccc tcctttataa
     1681 atacatcgca agttctaatg cttctaacag acccgcatat ccttggttat cgaattcacc
     1741 acttacttga tcaaagaata ttcctttatt ttcttcactt aatccgttaa cgttgtctaa
     1801 taaagcatta atgtcttctt cactaattgg ttctccattt agttgattgt ttttaatttc
     1861 ttctagttga ttttgcattt cttcaattgc cgctgcttgt tctggatcta aagtgttata
     1921 taatgatgga tcaaagaaat cttgttgtgt aatttcatca aatgcagctt gagcagcagc
     1981 taaagcagca agtaatgcaa gttgatcttc actaacacta acagtgatat taataccacc
     2041 attgtgaatc tttatcttat ccacagtaaa ggcactacta ccaccaacag tcattaactc
     2101 atagaatgag ttagctgata gtttaaatac accttgagcc tcatcatatt ttaatacatc
     2161 attatttgtg aagcttgcag aaagatagtt aagtaagaat tgcgaatcaa tattttttgt
     2221 tcctattaat aatgtaggac ttggtgtcaa aataacttct ggagaatcat tattgcttat
     2281 tgttaactct aaataagctc ttgtctttaa accattaatt tttactaagt attcaactgt
     2341 agtaatagga ctatagaaat ggaagataat tccctcaaca gtaatattta atgttgtttc
     2401 tccatcaggt agaagctcac tacttgctaa atctgcatat tgatgagtat cttgataaac
     2461 ttttctatta atatctaaat cagtaaaacg aatttcactt gttcctgctg tatcgctaaa
     2521 tagaattctt aaagcttgct ctgtaacaaa agattcttga acaaatggtt ctttaacttc
     2581 atctggtaaa gtttgtgttg ttttgaaatc aactaaatta agttttacat ttaaagcacc
     2641 gccagtaata ccttctacac caacaagttc aaatgttaag atttctggta aagtttcatc
     2701 aataattgta tcaatcacac cttcttcagt atttaaaaat gatgttaagt cactagtcat
     2761 aattgatatc gtattacctt gaattagaat aagttcattg tccccaacaa cttctcctaa
     2821 aatacctaag attgcctctt tagcttcagc actcatatga tagtttccta aactaatatc
     2881 tgttagttct aacttaacat ctgtagtaac taaagtatct tgttcaagac tagtgcttaa
     2941 taataacctt tgtccgttta aactcaatgc taagctaaaa ctcaatccat cattaacaac
     3001 atcacagaat aatgattcta cacctacaaa agtatatttt tcataagcgc tattagaacg
     3061 atataaagaa aaaccttcac caataattgg ggttgctgct aggatattat tgatagttgc
     3121 ctcatccaaa ccaatttgga ttaaaccatc acttaaatct ccaacctcac tatataattg
     3181 attgataata tcgtcttcaa tattttcatc gcttgtagaa atatttgcat aacctaaatg
     3241 cgcagtttta tcagctatac ctatactact aaaatcacaa gcattaatta ttgctcgatt
     3301 ttcttcactg atatttgcat aaccaactaa taagtaagaa ataattaaac ctgagttatc
     3361 attattaata acaccatttt catctaatgt taatattttt gttttgaaca cttcaaaatc
     3421 aaaccttgga gctgtaattt cgccatcata agcaaattca gtaacatcta agactaaccc
     3481 aattccatca ttttcgctta ttcctaaatt tattactcct ttagtatcaa taaaagtatc
     3541 taatagttct gatataatcg ttccataact agggttatcc atcgattcag ctaacatact
     3601 attaacttta gtttttatat cagccttagt taaagtaaat gataagtctt cccaagtaaa
     3661 tttaactgga atatctgatt cctcaaaatt attatttaac tctgcttcta agtcaatttg
     3721 aggcaaaaga atcttttcta ttgctaatct tcctagtcct gatgtaaggt taaatttacc
     3781 taattgtaat ttcttaacaa ctaatttaaa atctgtttct gtttgtttta tctctaattt
     3841 aagtgtaact aaaacatcaa tataactatc tacatgagca cgaatataaa gatatttgtc
     3901 tttaatttct ccataaacag atctaatttt aacttcaggc gaatcataga ttgccctgtc
     3961 ttcttcagtc ttataataat caggatttat ttcttgaaca ataagactat aaattaattt
     4021 attcaattca tcttcagtaa gtgttagctt aactttttga tcttctttcg tattcattaa
     4081 gcttttttga agtatatctt caaacgaaaa ttcctggttt tgcgctacat acaattcttc
     4141 tggtggattt gctgttccag ctgttagtaa taagtaaaat gttagaattg aaagtccaat
     4201 acacaatact atacccatga tggtagcaaa aataattttt atgacttttc tcataagcct
     4261 atcctttctt tatatatatt atatattttt ttagcacttt tagaaatact atctgcaaag
     4321 ttagtaatat aataatcaat tcctggaatt tttgaaggaa agttttgatt atcatattct
     4381 atcttttgat agattctttt aatagtatct cctcttttaa ccatcctcat ttttctttcg
     4441 gctaacgggg tttcgatata aaaagatacg atactgtctt ttaatatttt cttgatattt
     4501 aaaaatccta cttgatctaa aataataatt ttatcatctg ctatttcttt ataataagta
     4561 ccataataat cattattata tttagtatat tctataaata agttgtcttc tattcctgct
     4621 ttaaattctt cttctgttag gaagtaatag tctctaccgt ctttttcatc ccaacgcata
     4681 ggcctggtgg tacaagtaat tactcttttt aaattgtact ctttgattaa atagcgacta
     4741 atttctgtct ttcctgaagc actaggacct acaataacaa gcataactca cccactaatt
     4801 ttttctttct tttagtaata attcttcttt acaatcccaa agtttttgtg ataagtctac
     4861 atagtaatta tgtggttgat caaacctttt aacttcttcc caaagactat cgatttcttt
     4921 tcttacatgt tctcttattt ctaaaagatc aggcatttta taaactaatt tacctttttt
     4981 aaaaataggt attaataaag ctttagcttc aaatgtttct aaagtctttc ttttccaagt
     5041 tgcatatgga tcaaaaatct ctagtggctt actatcatct actacttcat catgaattgt
     5101 gatgtagtca gctgatgcct taccattttc gtataaacga tacacttgtt taaagtgtgg
     5161 tgttgtaatc ttatcgacac tttcgctaat tttgatttta ggaataatta caccatcttg
     5221 ttcaattgca gctaatttat atactccacc aaaaacaggc catgatttgg atgtaattaa
     5281 tctttcacct acaccaaaaa tatcaatctt tgctccttgt tctaaaagtg ccgtaatact
     5341 atattcatct aaagaattac taacaacaat cttacaatct gaataaccag ctttatctaa
     5401 aatctttcga agttgttttg attgataagc aaggtctcct gaatcaattc tcacacctaa
     5461 aggtcttttc cctaaaggtt ttaatatatt gtcaaaagcc ttaattgcat tataaatacc
     5521 ctgttttaaa gtatcataag tatcaattaa aagtaaactg ttatttggat acatcttaca
     5581 gtaagtttca aatgcttcaa gttctgtgtc aaacatttgc acccaagagt gagccatcgt
     5641 acctgatgct ggtataccaa ataaaacatc agctagtgta ttactagtgc tattaactaa
     5701 accaatatat gcagctctcg cacctttata tgcagcctct gctccatgag ctcttcttgc
     5761 tccaaactca gaaactactc ttcctttagc tgctcttaca acccgattag cttttgtagc
     5821 tattagtgat tgatgattaa aaacagctaa aacataggtc tcaattagtt gtgcttcaat
     5881 agcttttgct cttactgtca ttactggttc gccagggaaa aatactgatc cttcaggcat
     5941 cgcataaata tctccactaa atttaaaatc ttttaaatat tttaaaaact cttcagaaaa
     6001 aatattttta gatcttaaaa agtcaatatc ttttttagta aattttagat tttgaatata
     6061 ctctacaata tcttttaaac cagcgaaaat tgcaaagcca cctctatcag gaacatcacg
     6121 gtaatataaa tcaaaataaa caatttcatc tttttttcct gataagaaat agccattagc
     6181 catcgttagt tcataaaaat ctgtcatcat tgtataattc ataactaaca cctcttatca
     6241 tatattatac cataactact aaaataaaaa aagtttttaa gaataatctt aaaaacttat
     6301 ttaataaatt attttttagc cgcctgttct tctaatagtt ttttaatatc agctaataat
     6361 tcgtttgttg ttggtggaac aggagcagga tttgctaaag cttctaacct tgcttcttct
     6421 tcagctatta attcttcctc ataatgaaga agttctttac ctgcagcttt tttcttttgt
     6481 gctctcttta atgccttctc cttaccttta tcttgacctt gctttaatgc cattaatgct
     6541 ttaataatta aaaacaaaac aaaagcaata attaaaaagt taataacagc ttgagcaaaa
     6601 cttccccatc taaatagaat agcaccttca gatgctacaa aagcattagc tgctgcatcc
     6661 cattcatatg ttcctcttta agcgatagct ctatctgata tatctccacc accgaaagct
     6721 aagctaatta ctggcattaa aatatcagca acaaaactat taacaatttt accaaaagca
     6781 ccaccgataa ttacaccgac agccatatca acaatattac cacgttgaat aaattgtttg
     6841 aaatctctaa aaaattttct catctttttt cctttcttct taacacatct attataccat
     6901 agatttagga aaaaagttaa tttttatgct tttctaaatg ttttttaata gcctgaaaag
     6961 tttcgtcaga aatatcatgc tcaaacttac aagcatcttc ttcagcttgt tcttcgctga
     7021 cacctaaact aataaacact tttgtaaggg tagtgtgacg atcataaatt ttaaaagcaa
     7081 tctttttacc tttctcagta aaatctaaat atccattctc atcaataatt ataaatccat
     7141 catcttttaa agtatttaat gcttgtgata cagaagattt agcttttcca gtataattaa
     7201 ctatatcaac tgatcgaact tcttctgatt ctagttttaa aacatataat gtttctaaat
     7261 acatctcacc tgcttcaaaa attttcttat tcataaatac cctttcttag ttgttcttta
     7321 agttcgtcta aattagtttt tcttaaagca cattctttag tacaacttga acaactacta
     7381 cattctcctt ttttcttttt tctaaacgaa ttaacaacaa ccaaaactac aaagataaca
     7441 gcaactacaa ttactaaaat ttcaattaat gtcatgccgt cacctccact tttctctttt
     7501 gatactttcg atagttgatt tctataatcg ttattacaga tgccaaaatt gctaaaacaa
     7561 taaatattgc ccaccaccaa gtaccaatta cataaacaat tgtacttact aaatagctag
     7621 ttacaaacca aaagagaatt gtccaattaa aatctttcct tgtaagttca cccttagctg
     7681 atgccacagc tgcaaaacat ggaatcgtaa gcatattaaa agcaataaac gatataagtg
     7741 ctggaacagt aattcctgta gctccaatta tttgaattac agaagatacc ccttcctcat
     7801 ctccactaaa accagcaata attgcagctg ctaaagttcc aaatgtagca actacatttt
     7861 ctttagcaat caaaccagtt acaattgcaa ctgcaaacac ccatccatat tggttaagtt
     7921 gtgatccaaa accaagcggt gtaaataatg gttgaactaa ctgaccaaat ttacctaaaa
     7981 tactttcatt taactgcgaa tcttctaaaa atctccaacc gaatgagaaa tggctaattg
     8041 cccaaattac gattgtagaa gcaagaataa cagtgaatgc cttttgaata aaatgtttca
     8101 tcttatccca taagtgaatc attaaagaag aaaacttaat tgaacgatat ggtggaagct
     8161 ccattataaa tggagaatcc atacctttta aagttgtctt gctcataata acaattgcaa
     8221 taaatgcagt tactattcct aaaatataca ttccaaaagt aattaaatca gcatttccaa
     8281 ttccaaaatg cataacaatt gccccagcaa tcgccgttag gattggtagt tttgccccac
     8341 aactaaaaaa tggaataact ctaattgtag ctaatttttc ttcacgagag tttaaagtcc
     8401 ttgtattcat aattgctgga atactacaac caaaacccat aaccattggt attaatgctc
     8461 ttcctgataa acccattttc ttaaataatc gatcaagcaa gaaaacaatt cttgccatat
     8521 atcctgtatc ttctaaaatt gataagaaaa aataaagaat taagatttga ggtaaaaacg
     8581 atagaactgc aaataaacct gcaaatacac catcaactaa aaaccctaca agccaactag
     8641 cggcaccagc accttctaaa acacctgcaa accaccccat aaataaatcc cctaatgttt
     8701 caattaaggt tagaagcatt gccccaggag agttaattcc tccttcccca aacaatcctt
     8761 caaaactagt tccacttaag gtaacaaaat catccccaaa taatccacct aaaaacaaaa
     8821 agttctctga gaaagttaca tggaatacca aaaacataat aattaagaaa attggtatac
     8881 caaaaacagg atgagtaaag attttatctc ttttactact ttgtttaata atgtctaact
     8941 tttctttctt ttgaataaag atatctttac ttatcttttc aatagcctga tatcttgcat
     9001 cagcaatcat tccttcatta tcgtctttga atgttgcact ttgatattct ttactttttt
     9061 cttgaatctc ttcgtataat tttggatgat catttacttc aatttcatcc ccttcaatta
     9121 atttaattgc atgaaataat ggagaagaaa ttcctatttc ttctaactta gattctacta
     9181 aatcaattaa cccttttagt ttagttaatt taagtaaaga cttaggttct cttttcttac
     9241 ctgcttcttc aattgcaact tccattaatt ctttagtccc gctccccttt aaagcacaaa
     9301 tttccactgc cctaattcct aaatcttttt ctaaattagg aattgaaatt ttaatgtttt
     9361 ccctttcagc taaatccatc atattgacag ctaaaaccat aggtacacca agttccataa
     9421 tttgcgttgt aagatataaa tttctttcta aattagtaac atctacgata ttaataatac
     9481 aatctggttt actatgaaga ataaagtttc ttgtaattac ttcttcatca gtataaggtg
     9541 ataaagaata gtttcctggt aggtctgtta ctaagatctt ttctttacct cttttataaa
     9601 taccctcttt tttatcaact gttacaccag gccagttacc tacatgagca gttgatcctg
     9661 taatattatt aaatagtgtt gttttaccac tattagggtt accaacaatt cctatattaa
     9721 gcatcattac cctcctcatc taacatcttc atttctacta aactagcttc tgactttctt
     9781 agagttaaaa cataacctct taaaaacacc tctaacggat ctccaaatgg tgatttctta
     9841 ataaggctta ttttaacatc attagttatt cccatatcta agagtcttcg tctaatttgt
     9901 ccctttccta aaactgctat cacaatacca gtttcattaa tcttaaagtc tgataatttc
     9961 ttattgtgtt tcttcataaa gcctccaagt tcgtattgcc gaacaagagt attctatacc
    10021 ttttcaaaac aaatgtcaag catatttata aaaaagttac ttgaaattca agtaacttaa
    10081 ctttttctat tatagtaatc aattaaagcc tgcgttccac attcatcata tcctaattta
    10141 gataattctt caaacatatc tctaactgta gttaacatct ttagatctaa attattttct
    10201 ttagcctcat caatagctaa attcatatct ttaataaagt gtttaatata aaatcctggt
    10261 ctaaaatcat tagctaaagc ttttggtcct aagtttttca tttgccatga tccagcacta
    10321 ccttttgacc aaacttcgat taatggggca acatctaacc ctgctttttt cgcatacgaa
    10381 atagtttctg ccattcctga aattgctcct gcaatcgaaa tttgatttgc aagtttacta
    10441 tgctggccac taccagcttc tcctgtataa agaatagtcg ttcccattcc tttaaatact
    10501 ggtaaacact tttcataagc ctttttatct ccccctacaa agataaccaa agttccttca
    10561 attgctcctt tatctcctcc agttactgga gcatctaaag aaaatccact ttttttcttc
    10621 acgtcttgat aaattctctt agcaagttta ggacttgatg tagtcatatc aataactatt
    10681 ttgccttttc ctaagttatc aataatatta ccttccttat aatatacttc ttctaaatca
    10741 ctaggataac caactatagt aataataaca tcattagcca aagtaagatc tttgatactt
    10801 ggatagtatt taactccttg taacttatct tttgttttct cataatttct tgcataataa
    10861 gatatttcaa atcctttttt ctttaagttt aaaagcattg cacttcccat tacgc
//
LOCUS       DAOSGS010000020        10276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_5535_length_10276_cov_4.095588, whole genome shotgun sequence.
ACCESSION   DAOSGS010000020 DAOSGS010000000
VERSION     DAOSGS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_10276_cov_4.095588"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLQ22_01630"
     CDS             <1..712
                     /locus_tag="PLQ22_01630"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOH58693.1"
                     /translation="IAAFIASIYKRGMNYIYFPTSTTAMIDVAIGGKNGVNLLKSKNI
                     IGTITPPYKVVIDLSFLNSLPEKHYSNCLVEAIKMAMLFDKDLFKLFLKKDFKNIKEI
                     VIRSLKIKKEVVEKDEEDLEYRNLLNFGHTLAHAIEASSDYAISHGEAVARGILLDTL
                     NQDLKDKLKTIFKSLKIKYDYKLKIDKIKQYIYSDKKIKDEKIRIILLEDLEKYIVKY
                     VTVDEYIELLKEGVSHGF"
     gene            702..1688
                     /locus_tag="PLQ22_01635"
     CDS             702..1688
                     /locus_tag="PLQ22_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOH58694.1"
                     /translation="MAFSFGKNLTITIFGEAHSEKMGVVIEGLPVGKTINLESIKENL
                     ALRRSKDFFETKRVECDEFEIVSGYFNNKTTGAPLAILVSNAKQKSSDYNPDMIRPSH
                     ADFVSKVKYDGFNDYRGGGIFSGRLTVLLVIAGSICLELLKEENITIHTEVSSIGGMS
                     KGYEKLIEEAVSDNDSLGGIVKITVNNLPVGLGEPYFDKVDALLARGLFSVPSVKGVS
                     FGLGFEIAKLKGSEANDEFYYDGGYVKTKTNHSGGVNGGLTNGMPLVINVAIRPTPSI
                     GKEQNTISLKKSKNVKLEIKGRHDTIHVFRTQIVLKAVVALVIYDFMMKKLN"
     gene            1765..2811
                     /locus_tag="PLQ22_01640"
     CDS             1765..2811
                     /locus_tag="PLQ22_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58695.1"
                     /translation="MKKFLSFLVLVLVSVFLVSCGEPKKEEIQLSNQEVATMASAVSV
                     SVDGKDVISLESNIDLKVVLKGAEDSEVGDIELIAKGEVAMYADLDEFSSSYIYVKVD
                     AKYEINGNLEGFIFSLMGIDESELTEEDKANFLAMMNLDFPRNASVVGKVYVIAGVAY
                     VDATIITDGVELKVKRYEEVFSEEEFNEWKEELDSEKIEISEMDIEELLEVANLKTYK
                     VGDANLFEISLTKEKFEEAILAAIEEAGDYDAPVPDFTITHNGPFDAKLSVKFQETIE
                     EVKAAAKMNLGIEFAMDMGGGMNLEQKILVTNDFTFELNFKGKMPKGLPKAEDMKDYA
                     YGLDFDFLPDSRVR"
     gene            2915..4159
                     /locus_tag="PLQ22_01645"
     CDS             2915..4159
                     /locus_tag="PLQ22_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOH58696.1"
                     /translation="MYYLIGKKIGYSYSPYIHKNLGSYNYQVKEMNNISSLLSLKEFN
                     GLNITIPFKEEVVKYLDYLDPIVEEIGVCNTIVNNKGKLYGYNTDYAGFKYLLLKNNI
                     TIKDKTCYILGSGATSKTINFVLKELGAKKIVTVSRAKKGLDFLNYQDLKNDYYCEVL
                     INTTPVGVYPDFDKSVVSLKGFKKVETVIDVNYNPFRTKMLLEADSLGLKNYNGLEML
                     IYQAKVASELFQKKKIGDEIVQKIKKNLLFLRRNIVFIGMPGTGKTTIAKFVAQEYGK
                     KVYDVDKEVSKSEDMSITDLFKEKGEDYFRQKENEKIRELALKRGIVISTGGGVVLEP
                     KNMELLQANGLIVFLNRDLKNIYLRKGKRPLIQSKADLVKIKEERIGLYYKYKDLEVQ
                     NNYTIKESVKKVIKSLSDYRED"
     assembly_gap    4336..4435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4436..5317
                     /gene="aroF"
                     /locus_tag="PLQ22_01650"
     CDS             <4436..5317
                     /gene="aroF"
                     /locus_tag="PLQ22_01650"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOH58697.1"
                     /translation="ELVKLDLNQLKAFDAVEEVITLDTPYYQVLRKYQKENTVIKVKD
                     ANFGLGLEIIAGPCSVESKEQIDKIAKSLKAMGIKALRGGAYKPRTSPYTFQGLGEEG
                     LKYLKMAGEKYNLAIVSEIMSEDDLDLFIKYVDIIQVGARNMQNYALLKKLGKVKKPI
                     LLKRGFASTVDEWLLAAEYLLSMGNKEVILCERGIRTIANGSKATLDLNVIPHLKKIS
                     HLPVIVDPSHGTGLYEYVEPMSLAAIAAGADGLIIEVHYDPKAAFSDGVQTLSLERFE
                     KLLKKVKSLSEHLQEND"
     gene            5310..6548
                     /locus_tag="PLQ22_01655"
     CDS             5310..6548
                     /locus_tag="PLQ22_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58698.1"
                     /translation="MIRIKQSEAKGIVVVPASKSYLQRYILASFYSKDKIIIGNVNYS
                     NDVYDALNAIQALGKKVTLRKNEIVIEEGKKPSNEYRIKESATTLRLLIPILFTLKKT
                     CTIYCKESLLKRPLDYYKDLASKNKFYFKVEKDRIILKGSLKAGNYIIDSPKTSQFVT
                     GMLLTLPSLKGKSTLKVINQVSKPYVKMTLEALNNYGIKVDYQNDIYTIEGRQKYIGN
                     YWKTEGDWSQAAYFFALALFNEDLLIRNVNLDSLQGDKAILEILEDFGCEFEYSNGCF
                     KCIKRSFKKMTIDLNDIPDLGPILMVLALLGKEEVTFVNTNRLLYKESNRLDYMLFEL
                     SKIGLKYKKEDNQVTFLKSDLNNDSIVFETYNDHRIFMSLAILSIIVSNDTFIKGEGA
                     VNKSYPNFIEVLKELNVIIK"
     gene            complement(6630..6767)
                     /locus_tag="PLQ22_01660"
     CDS             complement(6630..6767)
                     /locus_tag="PLQ22_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58699.1"
                     /translation="MLISLLLLSVGTVGLGSLAVAAVSQVSRKTKDNWEYSRKEANQG
                     I"
     gene            6981..7778
                     /locus_tag="PLQ22_01665"
     CDS             6981..7778
                     /locus_tag="PLQ22_01665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOH58700.1"
                     /translation="MVKLIGIDMDGTLLDPQSKLSEENIKAIKYATSKGIDVVIASGR
                     PVCESMRDYYKLLGLKSYYVGFNGAVVYDFETDTILYQKGITGKDVKELEKFKSQFSS
                     VNSHAFILDTVLYEVEDYYVDLEVKVNYVKKKKIKFNDLADDFPVIKYMFTGSKVDID
                     NIINYVPEELKEKYNVVRSATYYLEFVNKEVDKARGIEIIAQKKGIAKKEIMTIGDEG
                     NDYLMVKEAGLGIAMGNARQKVKDVAKDITESNANNGVAHAIYKYIK"
     gene            7788..9203
                     /gene="pyk"
                     /locus_tag="PLQ22_01670"
     CDS             7788..9203
                     /gene="pyk"
                     /locus_tag="PLQ22_01670"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOH58701.1"
                     /translation="MRKTKIICTIGPASEKPEILRELMLAGMNCTRLNFSHGDHAEHK
                     KRIDTIKKIREELKLPVPILLDTKGPEIRVRDFKEGFIMLEEGQEFVFKPDDGSLGNE
                     KEVCITYPNLAKDVEIGNSILIDDGKIVLEVEKIVGENVHCRVQNSGRVSNHKSINVP
                     YVNINIPFLSKQDRLDIAFGVNQGIDYIAASFVRTKEDILGLREYLRSIGADDIKIIS
                     KIENMQGIENIDGIIDVSDGIMIARGDMGVEVHFSKLPPIQKEIINKCYLKGKLVVTA
                     TQMLDSMESNPRPTRAEVSDVANAIYDGTTCIMLSGETAAGKYPVESVKAMAAIAVEA
                     EDAIDYAQKYLENDLSLGSEVGQAIGNSAVISATQLHASAIIAITKKGTTARIVSSYR
                     PTTPIIACALDEQTCRQLYLYWNVLPIMAERKATTDDLFSHGLERAMSTGMLKKGDKV
                     AIVGASVAGDAAIDVLKLQIV"
     gene            9264..10274
                     /locus_tag="PLQ22_01675"
     CDS             9264..10274
                     /locus_tag="PLQ22_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH58702.1"
                     /translation="MIITERMTLGMKIVICIFLFMVLLNVVPPKKQLEKNPFIVAKGE
                     RPLIAAHRGGKNLNPENTFKAFDYAVNELDIDILELDLVMTKDKHLVAIHNLTINECS
                     DAEAISGSTEDYYVGAHTLAELQELNFGYNFKDRNGNYPYRYALDDPERMTKINNEQL
                     HIATIDDIFARYANTKLKFIVEIKDKNEIGFEAADQLNELLTVTYRENNLRERVIIGT
                     FNGEVENYIVEKYPTLFRGGSVSSVTSFVITQMLGMNIFDKSNFMALQIPTSRSVKGI
                     TIKLDQRTYIKRAHLRGISVQYWTINDKEEMRRLLELGADVIMTDNPDIALELLIEMG
                     YE"
BASE COUNT         3891 a         1080 c         1879 g         3326 t
ORIGIN      
        1 tattgctgcc tttatagctt caatttataa aagaggaatg aactatattt atttccctac
       61 ttctacaacc gcaatgattg atgtagctat tggtggtaaa aatggagtta acctattaaa
      121 aagcaaaaat attataggca ctattactcc gccttataaa gtagttattg atttaagttt
      181 tttaaatagt ttaccggaaa agcattatag caattgttta gttgaagcga ttaaaatggc
      241 tatgcttttt gataaagatt tatttaaatt attcttaaag aaagatttta aaaatattaa
      301 agaaattgtc attagatctt tgaagattaa gaaggaagtt gtagaaaagg atgaagaaga
      361 tttagaatat cgtaaccttc ttaattttgg tcatacttta gcgcatgcaa tagaagcaag
      421 tagtgactat gctattagtc atggtgaggc tgttgctaga ggaatacttt tagacactct
      481 taatcaagat ttaaaagata agttaaaaac aatctttaaa tctttaaaga taaaatatga
      541 ttataaactt aaaatagata aaataaaaca atacatttat tctgataaga aaataaaaga
      601 tgagaaaatt agaataattt tattagaaga tttagagaaa tatattgtta agtatgttac
      661 tgttgatgaa tatattgaat tattgaaaga aggagtttcg catggctttt agttttggaa
      721 aaaatttaac aattactatt tttggtgaag cacatagtga aaaaatgggt gtagtgattg
      781 agggcttacc tgtaggtaaa acaatcaatt tagaatcaat taaagaaaat ttggcattaa
      841 gaagaagtaa agattttttt gaaacaaaaa gggttgaatg cgatgaattt gagattgttt
      901 cgggttactt taataataaa actactggag caccactagc tattcttgtt tctaatgcaa
      961 aacaaaaatc ttctgattat aatccagata tgattaggcc atctcatgca gattttgtat
     1021 caaaagtgaa gtatgatggt tttaatgatt atcgtggagg agggattttt tctggtagat
     1081 taacagtttt attagttatt gctggttcta tttgtttaga attactaaaa gaagaaaata
     1141 ttacaattca tactgaagtt tcttctattg gtggtatgag caaaggttat gaaaaactaa
     1201 ttgaagaagc agtaagtgac aacgatagtt taggtgggat tgttaagata actgttaaca
     1261 atttaccagt tgggctgggt gaaccatatt ttgataaagt tgatgcttta cttgctaggg
     1321 ggttatttag tgtacctagt gttaaagggg tatcgtttgg tttaggattt gaaattgcta
     1381 aattaaaagg gtcagaagct aatgatgaat tttattatga tggtggttat gttaaaacta
     1441 agactaatca ttctggtggt gtaaatggtg gtcttactaa tggtatgcct ttagtaatta
     1501 atgtggctat taggccaaca ccttcgattg gaaaagaaca aaacacaata tctttgaaaa
     1561 agtctaagaa tgtgaaatta gaaataaaag gtcgtcatga tacaattcat gtttttagaa
     1621 cgcaaattgt tttaaaagca gttgttgcct tagtaattta tgattttatg atgaaaaaac
     1681 ttaattaaca agttctaaaa tgtaaacgat ttattgacaa aagacgaaaa tagtgtataa
     1741 taaatatgca aggagaaatg gattgtgaaa aaatttttaa gttttttagt attagtatta
     1801 gtatctgtat ttctagtatc ttgtggggaa ccaaagaaag aagaaattca attatctaat
     1861 caagaagtgg ccactatggc tagcgcagtt tctgttagtg ttgacggaaa agatgttatt
     1921 agtttagaat caaatattga tcttaaagtt gtattgaaag gtgctgaaga ttcagaagta
     1981 ggagatattg aacttattgc aaaaggcgag gttgctatgt atgctgatct tgatgagttt
     2041 agtagttcgt atatttatgt aaaggttgat gcaaaatatg aaattaatgg taatcttgaa
     2101 gggtttatat tttcacttat gggtatagac gaatcggaat taactgagga agataaagca
     2161 aatttcttag caatgatgaa tctagatttc cctcgtaatg catctgttgt tggtaaagtt
     2221 tatgttattg ctggtgttgc ttatgttgat gcaactatta ttactgatgg cgttgaatta
     2281 aaagttaaac gttacgaaga agtatttagt gaagaagaat ttaatgaatg gaaagaagaa
     2341 ttagatagcg aaaaaattga aatatctgaa atggatatag aagaattgtt agaagtagca
     2401 aatttaaaaa cttataaagt tggtgatgca aatctatttg aaatatcatt aactaaagaa
     2461 aaatttgaag aagctatttt agctgctatt gaagaggcag gagattatga tgcacctgtt
     2521 cctgatttta caataacaca taatggtcca tttgatgcta aactaagtgt taaattccaa
     2581 gaaactattg aagaagtaaa agctgctgca aaaatgaatt taggaatcga atttgcaatg
     2641 gatatgggtg gtggaatgaa tttagaacaa aaaattttag taactaatga ctttactttc
     2701 gaattgaatt tcaaagggaa aatgcctaaa ggcctaccta aagctgagga tatgaaagat
     2761 tatgcttatg gtttagattt cgatttcttg ccagatagta gagttagata aaaaagacaa
     2821 aaaaaataaa actgagatgc gaaagcacct cggtttttat tttttaatgg taaaataatg
     2881 caatttagag tataatatat aataagaggt atttatgtat tatttaattg ggaagaaaat
     2941 aggatattca tattcgccct atattcataa aaacttaggg tcatataatt atcaagtgaa
     3001 agaaatgaat aatatttctt ctcttttaag tttaaaagaa tttaatggtt taaatattac
     3061 gatacctttt aaagaagaag ttgttaagta tttagattat ctagatccga ttgtagagga
     3121 aattggagtt tgcaatacaa tagtaaataa caaaggcaaa ctatatggtt ataatactga
     3181 ctatgcgggt tttaaatatt tattattgaa aaacaatatt acaattaaag ataaaacttg
     3241 ttatatttta ggtagtggag ctacttctaa aactataaat tttgtactaa aggaacttgg
     3301 agctaaaaaa attgttactg tttctagggc taaaaagggt ttagatttct taaactacca
     3361 agatttaaaa aatgactatt attgtgaggt tttaataaac acaacaccag taggtgttta
     3421 tcctgatttt gataaatctg tagttagtct caaaggattt aaaaaagtag aaactgttat
     3481 cgatgttaat tataatccgt ttagaacaaa aatgttgtta gaagcagata gtctaggatt
     3541 aaagaattat aatggtttag aaatgctaat ttatcaagct aaggtggcta gtgagttatt
     3601 ccaaaagaaa aaaattggtg atgaaatagt tcaaaagatt aagaaaaact tgcttttctt
     3661 aaggagaaat attgttttta ttggtatgcc tggaaccggc aaaactacga ttgccaagtt
     3721 tgtagctcaa gaatatggta agaaagttta tgatgtagat aaagaagttt ctaaatcgga
     3781 agacatgagc attactgatc tttttaagga aaaaggtgaa gactatttca gacaaaaaga
     3841 aaatgaaaaa attagagaat tagctttaaa aaggggaata gttatttcta ctggtggagg
     3901 agttgtttta gaaccaaaaa atatggaact actccaagca aatggtttaa ttgttttctt
     3961 gaacagagat ttgaaaaata tttatttacg aaaaggtaaa agaccattaa ttcaatcaaa
     4021 agcagatttg gtaaaaatca aagaagaaag aataggatta tattataaat ataaagattt
     4081 ggaagttcaa aataattata ctattaaaga atcagttaaa aaagttatta agagtttaag
     4141 tgattatagg gaggattaaa atgattattg tattgaaaaa agctgaaatg aataatgtta
     4201 atgaattagt tgcttttttg gtatctaaaa agattgaaat aaactttttg gaagacaata
     4261 aagttttata tttaaaaggc gaactagtaa aacttgattt aaatcaattg aaagcttttg
     4321 atgctgtaga agaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaact
     4441 agtaaaactt gatttaaatc aattgaaagc ttttgatgct gtagaagaag taattacttt
     4501 agatactcct tattatcaag ttttaagaaa atatcaaaaa gaaaatacag ttattaaagt
     4561 aaaagatgca aattttggtt taggcttaga aattattgca ggtccttgct cggttgaaag
     4621 taaagaacaa attgataaaa ttgcaaaaag tttaaaagcc atgggtatta aagctttaag
     4681 gggtggtgca tataagccaa gaacatctcc ttatactttt caaggtttag gcgaagaagg
     4741 cttaaaatat ttaaagatgg cgggagaaaa atataatctt gcaattgttt cggaaataat
     4801 gtcagaagat gatttagatt tgtttattaa gtatgtagat attattcaag ttggagcaag
     4861 gaatatgcaa aactatgcct tacttaaaaa gttagggaaa gtaaaaaaac ctattttatt
     4921 aaaaagaggt tttgcatcta ctgtagatga atggctacta gctgctgaat acttattaag
     4981 tatgggcaat aaagaagtta ttctatgtga aagagggatt agaacaatag ctaatggttc
     5041 aaaagcaact ttagatttaa atgttattcc tcatttaaag aaaataagcc atttaccagt
     5101 aatagttgat ccttctcatg gcacaggttt atatgaatat gttgagccaa tgagcttagc
     5161 tgctattgca gctggtgctg atggtttaat tattgaagtt cactatgatc ctaaagctgc
     5221 ttttagtgat ggagttcaaa ctctaagttt agaaagattc gaaaaacttc ttaaaaaagt
     5281 aaaatcatta agtgagcatt tgcaagaaaa tgattaggat aaaacaaagc gaagctaaag
     5341 gaatagttgt tgttccagca tctaaaagtt atttgcaacg atatattctt gcttctttct
     5401 atagtaaaga taagattatc attggtaatg taaattattc taatgatgtt tatgatgctt
     5461 taaatgctat tcaagcctta gggaaaaaag tcacacttcg aaaaaatgaa attgttattg
     5521 aagaaggcaa aaaaccaagt aatgaatacc gcataaaaga atctgcaact acccttagac
     5581 tattaattcc cattcttttt actcttaaaa aaacttgtac aatttattgc aaagaaagtt
     5641 tattaaaaag acccttagat tattataaag atttagcttc aaaaaataaa ttttatttta
     5701 aagttgaaaa agatagaatc attttaaaag gctctttaaa ggctggaaat tatattatag
     5761 atagtccaaa gacaagtcaa tttgtaacgg ggatgttact tactttacca agtttaaaag
     5821 gaaaatccac tttaaaagtt attaatcaag tttctaaacc ttatgtaaag atgacgctag
     5881 aagctttaaa taattatgga atcaaggtgg attatcaaaa tgatatttac actattgaag
     5941 gcaggcaaaa gtacattggc aattattgga aaacagaagg tgattggtca caagctgctt
     6001 atttttttgc tttagcactt tttaatgaag accttttaat cagaaatgtt aatttggatt
     6061 cacttcaagg cgataaggct attctagaga ttttagaaga ctttggttgt gaatttgagt
     6121 atagcaatgg ttgctttaaa tgtatcaaaa gaagttttaa aaagatgaca attgatttaa
     6181 atgatattcc tgatttaggg cctattttaa tggtgcttgc tttgctaggt aaagaggaag
     6241 taacttttgt aaatactaat aggcttctct ataaagaatc taatcgttta gattatatgc
     6301 tttttgaatt atcaaaaata ggtttaaagt ataaaaaaga agataatcaa gttacgtttt
     6361 taaaatcaga tttaaataat gatagcattg tttttgaaac atataacgat caccgtatct
     6421 ttatgagtct tgcaatttta agtataattg tttctaatga tacttttata aaaggagaag
     6481 gagcggttaa taaatcttat cctaatttta ttgaagtgtt aaaagaatta aatgtgatta
     6541 taaaataaaa aaagccctaa agggcttttt gtttatatct aatcaaacta taaaaatagt
     6601 ttgttgttgt cagtaagttt tcaaggtaat taaattcctt gattagcttc tttgcgtgaa
     6661 tattcccaat tatcctttgt tttacgagaa acttgagaaa cagcagcaac tgctaaagaa
     6721 cctagaccta ctgtaccaac agataataat agtaaactaa ttaacatatt tctcacctct
     6781 ttcttacact agcattataa cacagttgtc aaatgaaaaa aagatgaaaa cgcaacgcat
     6841 tttcaatgaa aatactttca ttactttcat aaatgttttg aaaaagaaaa ttgttttttc
     6901 aagaaataat aatgaaaatg aaaacgatta aggagaattg caaaagacaa aatatatgat
     6961 ataatatatt gggtggaaag atggttaaat taattggaat tgatatggat ggtacattac
     7021 ttgatccaca atcaaaatta tcagaagaaa atataaaagc aatcaagtat gctactagta
     7081 agggtataga tgtagttatt gctagtggga gaccagtttg tgaatcgatg agagattatt
     7141 ataaactttt aggtcttaag tcatactatg ttggttttaa tggagctgtt gtttatgatt
     7201 ttgaaacgga tactattctt tatcaaaaag gtattaccgg taaagatgta aaagaactgg
     7261 aaaaatttaa aagccagttt agtagtgtaa atagtcatgc ttttatttta gacactgttt
     7321 tatatgaagt tgaagattat tatgtagatc tagaagtaaa agtaaattat gttaagaaaa
     7381 agaaaattaa atttaatgat ttagctgatg actttcctgt tattaaatat atgtttactg
     7441 gttctaaagt tgatattgat aatattatta attatgttcc agaggaattg aaagaaaaat
     7501 ataatgtagt tagaagtgct acttattatt tagaatttgt aaataaagaa gtagacaagg
     7561 cgagaggaat tgaaattatt gctcagaaaa aagggatagc taagaaagaa ataatgacta
     7621 tcggagatga aggaaatgat tatttgatgg ttaaagaagc tggcttaggt atagcgatgg
     7681 gaaatgcgcg ccaaaaagta aaagacgtag ctaaagatat tacagaatca aatgcaaata
     7741 atggggtagc acatgctatc tataaatata ttaaatagga ggaaaatatg agaaaaacta
     7801 aaattatttg tacaataggt ccagcatcag aaaaaccaga aatattgaga gagttaatgc
     7861 ttgccggaat gaactgcact cgtttgaact tctcacatgg agatcatgca gaacacaaaa
     7921 aaagaattga cactattaaa aaaattagag aagaattaaa attaccagta cctattctac
     7981 tagatacaaa ggggccagaa attagagttc gtgactttaa agaaggattt atcatgttag
     8041 aagaaggaca agagtttgtt tttaaaccag atgatggtag tttgggtaat gagaaagaag
     8101 tttgtatcac ttatccaaat ttagcaaaag acgtagaaat aggaaatagc attttaattg
     8161 atgatggaaa aatcgtatta gaagtagaaa agattgttgg tgaaaatgtc cattgtcgtg
     8221 tgcaaaacag cggtagagta agcaatcata agagtattaa tgttccttat gtaaatatta
     8281 acattccatt tttaagcaaa caagatcgtt tagatattgc ttttggtgtt aatcaaggga
     8341 ttgattatat tgcagcttca tttgtaagaa caaaggaaga tattttagga ttaagagaat
     8401 atttaagatc aattggtgca gacgatataa aaattatttc taaaattgaa aatatgcaag
     8461 gtattgaaaa tattgatggt attattgatg tttcagatgg aattatgatt gctagaggag
     8521 atatgggagt tgaagttcat ttctcaaaac tacctccaat tcaaaaagag attattaata
     8581 aatgttattt aaaaggtaaa ctagtagtta ctgctacaca aatgttagac tctatggaat
     8641 caaatccaag acctacaaga gcagaagtta gtgacgtagc taatgccatt tatgatggaa
     8701 ctacttgtat aatgttatct ggagaaactg cagctggtaa atatccggtt gaatcagtaa
     8761 aagcgatggc agctattgct gttgaagcag aagatgcaat tgactatgct caaaaatatc
     8821 ttgaaaatga cttgagctta ggttcagaag taggacaagc aattggtaac tcagcagtta
     8881 tttcagctac acaacttcat gcaagtgcaa ttatcgcaat tactaaaaaa ggaacaactg
     8941 caagaattgt ttcttcatat agaccaacaa caccaattat cgcttgtgct ctagatgaac
     9001 aaacatgccg tcaattatac ttatattgga atgtattacc aattatggct gaaagaaaag
     9061 caactacaga tgatttattt agccatggtc ttgaaagagc aatgtcaaca ggaatgctta
     9121 agaagggtga taaagttgcg attgtaggtg catctgttgc aggcgatgct gcgattgatg
     9181 tattaaaatt gcaaatcgta taaaaaagaa gcacaagctt ctttttttat ggtataatat
     9241 aacataagta aaaggaggac gagatgatta ttactgaaag aatgacctta ggaatgaaga
     9301 tagttatttg tatattttta tttatggtat tactaaatgt ggtaccacct aaaaaacaac
     9361 tagagaaaaa tccttttatt gtagcaaaag gagaaagacc attaattgca gcccatcgtg
     9421 gcgggaaaaa tttaaatcca gaaaacacat ttaaagcttt tgattatgca gttaatgaat
     9481 tagatattga tattttagaa ttagatcttg ttatgactaa agataaacat ttggttgcta
     9541 ttcataatct aacaattaat gaatgctcag atgcagaagc aatttctggt agtactgaag
     9601 attattatgt aggtgctcat acactagctg aactacaaga attaaatttt ggttataatt
     9661 ttaaagatcg taatggtaac tatccgtacc gttatgcact tgatgatcct gaaagaatga
     9721 caaagattaa taatgaacaa ttacatatag ctacaattga tgatattttt gctcgttatg
     9781 ctaatactaa attaaagttt attgttgaga taaaagacaa aaatgaaata ggttttgaag
     9841 ctgcagatca attaaatgaa ttattaactg ttacttatag agaaaataac ttaagagaaa
     9901 gagttattat tggaacattt aatggcgaag ttgaaaatta tattgtagag aaatacccaa
     9961 ctttattccg tggcggtagc gtatcttctg ttacatcatt tgtaattaca caaatgctag
    10021 gaatgaatat ctttgataag tcaaatttta tggctttaca aattccaaca tcaagatcag
    10081 ttaaaggaat tactattaaa ctagatcaaa gaacatatat taaaagagct catttaagag
    10141 gaatatcagt tcaatactgg actattaatg ataaagaaga aatgcgtaga ttattagaat
    10201 tgggagcaga tgttattatg actgacaatc ctgatattgc tttagaatta ttaatagaaa
    10261 tgggttacga ataatc
//
LOCUS       DAOSGS010000021        10045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_5711_length_10045_cov_5.043744, whole genome shotgun sequence.
ACCESSION   DAOSGS010000021 DAOSGS010000000
VERSION     DAOSGS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_10045_cov_5.043744"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLQ22_01680"
     CDS             <1..1164
                     /locus_tag="PLQ22_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH58703.1"
                     /translation="SYIEKMEISGYSVEVKEYLDIDAFLNYYLQNKVTEPISISYRNN
                     GESKQVFVSPQYVLYNLGIYSDQRTEEVVIGYIAAVDDNMSTNKGLLSGDKIITINGE
                     DISNWLDVVKFANNYVGGTGTDADNLNFIVLRESNTVLANPPKNTEATIVTSSNEDIF
                     EVLVKRIDDKETIEIVPKTIGTANIKFTYTLENGSTVTDQYPVTIIEKDGKLSARQTV
                     SVKVKPYSKSIMDTQYDIERNKIRPAGVALGFLPSREFSLHKSLVFGIDQTVNSSTVV
                     FSTLRMLFKDKTVTIKSLSGPVGIYKITSQFASLGFLPLLGLLGMLSVNVGLLNLFPI
                     PALDGGRIVFLGYEAITRKKPNPKVEKILITVTMFLLLGLIAFVTLNDIIKLF"
     gene            1175..2404
                     /gene="proS"
                     /locus_tag="PLQ22_01685"
     CDS             1175..2404
                     /gene="proS"
                     /locus_tag="PLQ22_01685"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH58704.1"
                     /translation="MKQSRFFIPTLRETPKDAQIVSHRILLKGGYIKQVAAGIYDYLP
                     LAYKIIKKIENIVREEINKTESVEVLMPALQPKDLWVESLRWEQYGKELMRLEDRNER
                     PFCLGPTHEEVVTDLVRDYVNSYKKLPIGLYQIQNKFRDEFRPRFGLMRGREFLMFDL
                     YTFHKDEDDLDVWYKRVREAYKRILNRLELDYRIIGADSGNIGGKASEEFMVLCDVGE
                     DTIVYSDSSSFALNQEICDLKEGDPSPDGKGVIKHAKGIEVGHIFKLGTRYSELMKAN
                     FIDQDQKTKPIIMGCYGIGISRLLMAILEQHSNDEKPVWPKEVTPFDIHIIPLGDNLD
                     QGLEIYEKLSKNYDCLIDDRDERAGVKFQDADLVGVRYRIVVGRKADEGIYEFQDLKQ
                     GIKKELKLSEIEKELKK"
     gene            2401..3540
                     /gene="nagA"
                     /locus_tag="PLQ22_01690"
     CDS             2401..3540
                     /gene="nagA"
                     /locus_tag="PLQ22_01690"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOH58705.1"
                     /translation="MKGFTNTNIYVEGKGIIKTSLEIENGKIKSIGKFKDGIELPDNL
                     IVVPGFIDQHIHGANHSDSMYPTKKDILNIATSITQDGVTGFLPTTMTQSIENINKAL
                     ENIKKYIEANVEEGAEVLGIHLEGPFVSKKQKGAQPEEYILAADVAAFKKFEKVSGNN
                     IKIVTMAYEENGKELTKYLKSKKIVAALGHTDASYDVASEAVENGATLFTHCYNAMRG
                     LHHRDAGTLGAAMLHDEAYVELICDLIHVSPAAIKILYKQKPKDKFILVTDSMEAKHL
                     PDGKYQLGGQDVYVKGGAARLETGNLAGSVLKMNEAIRNCKETLEISLEEAIDFATIN
                     PARNLNISNKKGSIKVGKDADFAIIDQDLNVYETIRAGKTIYKKQ"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3737..4375
                     /locus_tag="PLQ22_01695"
     CDS             <3737..4375
                     /locus_tag="PLQ22_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58706.1"
                     /translation="LSALAVGFFFMILNGLIKRSNYKRKGLYNFLLGLAFIVLFLGSY
                     FYDKYIFEITDFTKYYALLGSSAFYTLVLAGYNFIKGIQRNERVFKGTKSSKPKDFIY
                     ILLKYQDGFLLEKTNEKYLAIVRKMKTNFHDEDVSNFVKSYGECLNVSYKGVVYKKDY
                     SYYCYLIETNTYNQKLEFVNQFELTQIMMSAEDKEIVLSIILRNDFILDKRI"
     gene            complement(4400..5623)
                     /gene="hflX"
                     /locus_tag="PLQ22_01700"
     CDS             complement(4400..5623)
                     /gene="hflX"
                     /locus_tag="PLQ22_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH58707.1"
                     /translation="MKGILVGVNTTDLELEELQGIAEACSIEVCHTITQNLEKINPAH
                     YVGKGKLEEIKMALDFYKAEVVIFNNELSPSQYVNIEDHLKCIVYDKTYCILEIFNQR
                     AQTKEAKIQTEIATLNYMLPRLIAVNKNLSRQVGSGGGLYLRGGGETVQELKRRNVRQ
                     RLSDLNKTLKEIKKTNETKKTKRNKSFLKQVALVGYTNSGKSTTLNLFLEDEEKLVIT
                     KDMLFTTLDTYTRIVDINHHKILLSDTVGFISDLPKQLVDAFLSTIIEVKEADLIIQV
                     VNGSNPNFEEEIENTNKILKQIGANQIKKIYLINKVDLVEHALIIPSKYENVIKFSAK
                     TKENFNHLVDAIEDKLFSNEYFIKVEIPLEESKLINDLKKQGQVIKIIYNQTSVYLEV
                     LVNKQLFDQIIKYKK"
     gene            complement(5664..5966)
                     /locus_tag="PLQ22_01705"
     CDS             complement(5664..5966)
                     /locus_tag="PLQ22_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58708.1"
                     /translation="MKKKIMAALPIMCTIAYLLMGFLADLWHPGWIVFLLVPLVSAIL
                     NSTFKFVTFYTILVVIAYMIIGFTIDGWHPWWILFLTIPIVSIFAKPDKKKEKDNN"
     gene            complement(5982..6416)
                     /locus_tag="PLQ22_01710"
     CDS             complement(5982..6416)
                     /locus_tag="PLQ22_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58709.1"
                     /translation="MKLYLKGRIMAALPIVCTIAYLLMGFLADLWHPGWVVFLAIPLF
                     AAILFNKNLKLVSIYTTVISVAYVVTSALTGLWHPLWIAFLTIPIFSIFTRPRHLTAE
                     EFCDMVENQNDDCDCGYDAEWEEKIEKEFEEKFDSKDEESKK"
     gene            complement(6426..8963)
                     /locus_tag="PLQ22_01715"
     CDS             complement(6426..8963)
                     /locus_tag="PLQ22_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOH58710.1"
                     /translation="MQENVKYTKELKQKLNEDLKNYFPHLFLVDDDMEKSFTSVSRLI
                     LLDRYAQKDINHISYSKGDLVLCVVKDDPKFPSRGIGYIDRITKNHVYIKLEEEYVAM
                     AEDANNKGIIKRKIGEVDKPLELYYEQVCQRVANNLSIGETLEVVQALEAELKEMNIV
                     PAGRVLFGAGSNTAVTYFNCFVMPYIHDSRGGISRHRKEVMEIMSRGGGVGTNGSTLR
                     PRNALAKGVSGKSSGSVSWLNDLSSLTNLVEQGGSRRGAQMIMLADWHPDILEFIISK
                     MQNPKILLFLIDNLEDEDIRRLAKDKLKFTHLSREEIEIYETIVKLAETNPDLVSKLA
                     LQKVKDTLNLGGTYSVNNPDFLSGANISVAITKEFMHAVENDLDYALKFPDTDDYSPG
                     EKAAYNEKWQACGDVREWEKMGYRVKTYRTIKARELWHLICICATYSAEPGIFFIDNA
                     NDMTNAKAYGQKVVCTNPCGEQPLAPYSVCNLAALNLANMYDWEKRDVNWDKLENSIR
                     VAVRMQDNVIDQTTYFLEANEKQAKGERRVGLGVMGLHDLIIYANLRYGSKEGNKFVD
                     KLFKFIATTAYDASIDLAKEKGSFPFLDQYGSREKFIETGYMKSMPQKIRNDVLKYGI
                     RNSHLLTIAPTGSTGTMVGVSTGLEPYFAFKYYRSGRLGQFMEVNAPVIDIYLSHNPE
                     YSYDNLPDVFVSAMGLTAEEHADTQCVIQKWVDSSISKTVNAPKGYTVEDVESLYFRL
                     YKGGAKGGTVYVDGSRDSQVLTLTKEDKLPKQVGLEDLGIEITESKSDVKSESADSLN
                     QVAPLKANREIGVEVGNLCPICHEGIVEEIGGCNTCTNCNAQLKCDI"
     gene            9197..>10045
                     /locus_tag="PLQ22_01720"
     CDS             9197..>10045
                     /locus_tag="PLQ22_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58711.1"
                     /translation="MIQGSNRKMRLSNSATYLQDIFSNTPYIELGNIDYEIMEKILNF
                     ERIRIEEISDFNIQNSGMSNFKINRESLQNIIPYMFIDSLNKKNRVYQLFLTYGLVRY
                     RDNNKEEKFAPIVLIPVNLYFENNQIYLQKYARPIANDILINILSTILTSDQRRLLPT
                     QVLDRFDSLYSIDRYILQLANIEGLDVKLENYLTIAKTVADRPDLKHSKFPIVRDQNE
                     ALLDKLYSKECNLRYIAPLNKKQREALYNASIGNSFVIVGRLGTGKTLTLTNICLEAI
                     TQGKRIL"
BASE COUNT         3457 a         1429 c         1582 g         3477 t
ORIGIN      
        1 tcttatattg agaaaatgga aataagtggt tattcagttg aagtaaaaga atatttagat
       61 atcgatgctt ttttaaacta ctatttacaa aataaagtta cagaaccaat ttctattagt
      121 tatcgcaata atggtgaaag taaacaagta tttgtttctc ctcaatatgt cttatataat
      181 ctgggtattt atagcgatca aaggactgaa gaagtagtta taggttatat tgcagcagtt
      241 gatgataata tgagcacaaa caaaggttta ctttcaggtg ataaaattat aacaattaat
      301 ggagaagata ttagtaactg gctagatgtt gttaagtttg ctaataacta tgtaggtgga
      361 actggaaccg atgcggacaa tttaaatttt atagtgttaa gagaatctaa tacagtatta
      421 gctaacccac ctaaaaatac agaggcaaca attgtaacat catcaaatga agatattttt
      481 gaagtattag taaaacgcat agatgataaa gaaacaattg agattgttcc aaaaacaatt
      541 ggtactgcaa atattaaatt cacttatact ttagaaaacg gttcgacagt tactgatcaa
      601 tatccagtta caattataga aaaagatggt aaattaagcg caaggcaaac tgtaagtgta
      661 aaggttaaac catatagcaa aagcattatg gatacacaat atgatattga gagaaataaa
      721 attcgtccag ctggtgttgc tttaggattt ttaccaagtc gcgaatttag tttacataaa
      781 tcacttgtct ttggaattga tcaaactgtt aactcttcaa ctgtcgtttt tagtacttta
      841 agaatgttat tcaaagataa aacagtaaca attaagtcgc tatctggacc ggttggtatt
      901 tataaaataa ctagtcaatt tgcttcactt ggctttttac cactattagg tttactgggt
      961 atgctatcgg tgaatgttgg actattaaac ttgtttccaa ttccagcact tgatggtggt
     1021 agaatagtgt ttttagggta tgaagcaatt acaaggaaaa aaccaaatcc aaaagtggaa
     1081 aagattttaa ttacagtaac aatgttttta ttattaggct taattgcctt tgtaacattg
     1141 aatgatatta ttaaattatt ttaggaggta aataatgaaa caaagcagat tttttatccc
     1201 aacattaagg gagacaccga aagatgccca aatagtatcg cataggattt tattaaaggg
     1261 tggatatatc aaacaagtcg cagctggtat ttatgattat ttacctttag cttataagat
     1321 tattaaaaag attgaaaata ttgttaggga agaaataaat aaaacagaat cagttgaagt
     1381 tttaatgcct gctttgcaac caaaagattt atgggttgaa tctttaagat gggaacaata
     1441 cggaaaagag ttaatgagat tagaagatcg caatgaacgt cctttttgtt tagggcctac
     1501 tcatgaagaa gtagtaactg atttagttag agattatgta aatagttata aaaaactacc
     1561 aataggttta tatcaaattc aaaataagtt tagagatgaa tttcgtcctc gttttggttt
     1621 aatgagagga agagaatttt taatgtttga cttatacact tttcacaaag atgaagatga
     1681 tttagatgtt tggtataaaa gggttagaga agcttataag agaatcttaa atcgacttga
     1741 attggattac cgtattattg gagccgattc aggaaatatt ggtggtaagg cttcagaaga
     1801 atttatggtg ctatgtgatg ttggagaaga tacgattgtc tatagcgata gttctagttt
     1861 tgctttaaat caagagattt gtgatttaaa ggaaggagat ccttctcctg atggtaaagg
     1921 tgtaattaaa catgcaaagg gaattgaagt tggtcatatt tttaaacttg gaactagata
     1981 ttctgaatta atgaaggcta actttattga tcaagaccaa aaaactaaac ctattattat
     2041 gggttgttat ggaattggta tttctcgttt attaatggct attttagaac aacactcaaa
     2101 cgatgaaaaa ccagtttggc caaaagaagt tactccgttt gatattcaca ttattccact
     2161 aggtgacaac ttagatcaag gtttagaaat ttatgagaaa ttatctaaaa actatgattg
     2221 tttaatagat gatcgtgatg aaagagcggg agttaaattc caagatgcag atttagttgg
     2281 ggtaagatat agaattgtag ttggccgtaa agctgatgaa ggaatatatg aattccaaga
     2341 tttaaaacaa ggcattaaga aagaacttaa attatcagag atagaaaagg agttgaaaaa
     2401 atgaaaggat ttactaatac aaatatttat gtagagggaa aagggattat aaagacttct
     2461 ttagaaattg aaaatggaaa aattaaatct attggaaaat ttaaagatgg catagaactt
     2521 ccagataact taattgtagt tccaggattt attgatcaac atattcatgg tgctaatcat
     2581 tcagattcaa tgtaccctac aaaaaaagat attttaaata ttgcaacatc aataactcaa
     2641 gatggagtta ctggtttttt accaacaaca atgacgcaaa gtattgaaaa tatcaataaa
     2701 gctttagaga atattaaaaa atatattgaa gcaaatgtag aagaaggagc agaagtttta
     2761 ggaattcact tagaaggacc tttcgtaagt aaaaaacaaa aaggagcaca accagaagaa
     2821 tatattttag ctgcagatgt tgctgcgttt aaaaaatttg aaaaggtaag tggtaataat
     2881 attaaaattg ttactatggc ttatgaagaa aatggtaaag agttaactaa atacttaaaa
     2941 tcaaaaaaga ttgtagctgc cttaggtcat acagacgcat catacgatgt agcaagtgaa
     3001 gcagtagaaa atggtgcaac attatttaca cactgctata atgcaatgag aggtttacac
     3061 catcgtgacg ctggtacttt aggtgctgct atgcttcatg acgaggctta tgtagagtta
     3121 atttgtgact taattcacgt tagtcctgct gctattaaaa tcttatataa acagaaacca
     3181 aaagataagt tcattttagt aactgactct atggaagcaa aacatttgcc tgatggtaaa
     3241 taccaattag gcggtcaaga tgtttatgta aagggtggtg ctgcaagact agaaactggt
     3301 aatcttgcag gttcagtttt aaaaatgaat gaagctatta gaaattgtaa ggaaacttta
     3361 gagatttctt tagaagaagc aattgatttt gcaacaatca atccagcacg taatctaaac
     3421 ataagcaata aaaaaggatc gattaaagta ggcaaagatg cagactttgc aattatcgat
     3481 caagatttaa atgtttatga aacaattaga gcaggaaaga caatttacaa aaaacaatga
     3541 ttttacaaat tgaagttctg aatttactta aaattatctt tttaagtgct ttagcagttg
     3601 gatttttctt tatgatttta aatggactta ttaagannnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnttaa gtgctttagc agttggattt ttctttatga ttttaaatgg
     3781 acttattaag aggtctaact ataaaagaaa aggtttatat aattttcttt taggtcttgc
     3841 ttttattgtt ttgtttttag gcagttattt ttatgataaa tacatttttg aaattactga
     3901 ttttactaaa tattatgcct tacttggttc tagtgcattt tatactttag ttttagctgg
     3961 atataatttt attaaaggta tccaaagaaa tgaaagagta tttaaaggaa ctaaatcatc
     4021 aaaacctaaa gattttattt atattctact taagtatcaa gatggatttt tattagaaaa
     4081 gacgaatgag aaatatcttg ctattgttag gaagatgaaa actaattttc atgatgaaga
     4141 tgttagtaat tttgtaaaga gttatggtga atgtttgaat gttagttaca aaggtgtagt
     4201 gtataaaaaa gattatagct attattgtta tctaatagaa acaaacactt ataatcaaaa
     4261 gttagaattt gtgaatcaat ttgaacttac acaaataatg atgagtgctg aagataaaga
     4321 aatagtatta tcaattattc taagaaatga ttttatttta gataagagaa tataaaaaag
     4381 cttgttttaa aacaagcttt tattttttat atttaattat ttgatcaaat agttgtttat
     4441 taactaaaac ttctaaataa actgatgttt gattataaat tattttaata acttggcctt
     4501 gttttttaag atcgttaatt aatttacttt cttctaaagg aatttctact ttgataaagt
     4561 attcgttaga aaatagttta tcttcgatag catctactaa gtgattaaag ttttctttag
     4621 ttttggcact aaatttaatt acattttcgt atttactggg aataattaaa gcatgttcta
     4681 ctaaatctac tttattgatt aagtagattt ttttaatttg gtttgcgcca atttgcttta
     4741 agattttatt tgtgttttca atttcttctt caaaattagg gttagagcca ttaacaactt
     4801 ggataattaa gtctgcttct ttaacttcaa taattgtaga tagaaaagca tctactagtt
     4861 gttttggtaa atcactaata aaaccaacag tatctgaaag taagatttta tgatggttga
     4921 tatctactat tcttgtataa gtatctagtg tagtaaataa catatcttta gtaattacta
     4981 gtttttcttc atcttctaag aaaagattta aagtagtaga tttaccacta ttagtataac
     5041 caactaaagc aacttgtttt aagaaagatt tatttctttt agttttttta gtttcattag
     5101 ttttcttaat ttcttttaaa gttttattta agtctgaaag tctttgcctt acatttcttc
     5161 tttttaattc ttggacagtt tcaccaccac cacgaagata taaaccacca ccagaaccta
     5221 cttgacgcga taagttttta tttactgcga ttaatcttgg taacatatag tttaaagttg
     5281 caatttctgt ttggattttg gcttctttag tttgtgctct ttggttaaag atttctagaa
     5341 tgcaatatgt tttatcataa acgatacatt taaggtggtc ttcaatattt acgtattgtg
     5401 atggggaaag ttcattatta aaaataacaa cttcagcttt ataaaagtct aaagccattt
     5461 tgatttcttc tagtttacct ttacctacat aatgagcagg attgattttt tctagatttt
     5521 gagtaattgt atgacaaact tcaattgaac atgcttcagc aatcccctgt aattcttcta
     5581 gttctaaatc agttgtattt accccaacaa gtattccttt catataatcc tcctttttaa
     5641 aaagggtaaa aatacccttt tttttaatta ttatcttttt ctttcttttt atcaggtttt
     5701 gcgaaaattg acacaatagg gatagttaga aataaaatcc accaaggatg ccaaccatca
     5761 attgtaaagc caattatcat ataagcaatt actactaaaa ttgtatagaa tgttacaaat
     5821 ttaaaagtac tatttaaaat cgcacttact aggggaacaa gtaagaaaac aatccaacct
     5881 ggatgccata agtcagctaa aaatcccatt aataaataag cgattgtaca cataattggc
     5941 aatgcagcca taattttctt tttcatatat tctctcctta attatttttt gctttcttca
     6001 tctttagaat cgaatttttc ttcaaactct ttttctattt tttcttccca ttcagcatca
     6061 tatccacaat cacaatcatc attttgattt tctaccatat cacaaaattc ttcagcagtt
     6121 aaatgtctag gtcttgtgaa gattgaaaag attggaatag ttagaaaagc tatccataaa
     6181 ggatgccata aaccagttaa ggcacttgtt actacataag caacgctaat tactgttgtg
     6241 tagatagaaa caagttttaa gtttttatta aataggattg cagcaaataa aggaatggct
     6301 aagaatacaa cccagcccgg atgccataag tctgccaaga agcccattag taaataagca
     6361 attgtgcaga caataggcaa agcagccata atcctacctt ttaaatatag tttcataaaa
     6421 tctccttaaa tatcacattt taattgggca ttacagtttg tacaagtgtt acaaccacca
     6481 atttcttcta caatcccctc atggcaaata ggacataagt tacctacttc aacaccaatt
     6541 tcacgattag cttttaaagg tgctacttga tttaaactgt cagctgattc agattttaca
     6601 tctgatttag attctgtaat ttcaatacct aagtcttcta aacctacttg ttttggaagt
     6661 ttatcttctt ttgttagagt aagaacttga gagtctctag atccgtctac ataaacagta
     6721 ccacctttag ctccaccttt gtaaagtcta aagtataaac tttctacatc ttcaactgta
     6781 tagcctttag gtgcgttaac agtttttgag attgaagagt caacccattt ttgaattaca
     6841 cattgtgtat ctgcgtgttc ttcagctgta agccccatag cacttacgaa aacatctggt
     6901 aaattatcat atgaatactc agggttgtga cttaagtaga tatctattac aggagcatta
     6961 acttccataa attgtcctaa acgaccacta cgatagtatt taaatgcaaa gtatggttct
     7021 aagcctgttg atactccaac cattgttcct gtagaaccag taggagcaat tgtaagtaaa
     7081 tgagagtttc taataccata ttttagaaca tcgtttctaa ttttttgtgg catactcttc
     7141 atataaccag tttcgataaa tttctctctt gaaccatatt gatctaaaaa tggaaagctt
     7201 cctttttctt ttgcaaggtc aattgaagca tcataagcag tagtagcaat aaacttaaat
     7261 aacttatcta cgaacttatt accttcttta ctaccataac gaaggttagc ataaatgatt
     7321 aagtcatgta ggcccataac tcctaaacca acacggcgtt cacctttagc ttgtttttca
     7381 tttgcttcta agaaataagt agtttggtcg attacgttat cttgcattct aactgcaact
     7441 ctaattgagt tctctagttt atcccagtta acatctctct tttcccagtc atacatatta
     7501 gctaagttta aagctgctaa gttacaaact gagtaaggag caagtggttg ttccccacaa
     7561 gggttagtac atacaacttt ttgcccatat gcttttgcat tagtcatatc gtttgcatta
     7621 tcaataaaga aaatgcctgg ttcagctgaa tatgtagcgc aaatacaaat taaatgccat
     7681 aactcacgag ctttaattgt gcgatatgtt ttaacacgat agcccatttt ttcccattct
     7741 ctaacatccc cgcaagcttg ccatttttca ttataagcag ctttttcacc cggagaatag
     7801 tcatctgtat ctgggaattt taaagcatag tctaaatcat tttctactgc atgcataaac
     7861 tcttttgtga tggcaacaga aatatttgct ccacttaaaa agtcagggtt atttacagaa
     7921 taagtacctc ctaaatttag agtatcttta actttttgta aagcaagttt gcttactaaa
     7981 tcagggttag tttctgctag ttttactatt gtttcataaa tttcaatttc ttcacgactt
     8041 aggtgagtaa actttagttt gtctttggcc agtcttctaa tatcttcatc ttctaagtta
     8101 tcaattaaga aaagtaaaat ctttggattt tgcattttac taatgatgaa ttctagaata
     8161 tctggatgcc agtcagctaa cataatcatt tgggctccac gacgacttcc accttgctct
     8221 acaagatttg taagggaaga taagtcgtta agccaactta ctgagcctga agatttacca
     8281 ctaacgcctt tagctagagc attacgagga cgaagagtag agccgttagt tcctacacca
     8341 cctccacgag acataatttc cataacctct ttacgatggc gactaatacc gccacgagag
     8401 tcatggatat atggcataac aaagcagttg aagtaagtaa cagctgtatt actgccagca
     8461 ccaaaaagca ctctaccagc aggaacgata ttcatttctt ttagttctgc ctctaatgct
     8521 tgaacaactt ctagagtttc tccaatactt aaattgttag ctactctttg gcaaacttgt
     8581 tcatagtaaa gttctaatgg tttatcaact tcaccaattt ttcttttaat tattcctttg
     8641 ttatttgcat cttccgccat tgctacgtat tcttcttcaa gtttaatata aacatgattt
     8701 ttcgtaatac gatcaatata accaatacct ctagaaggga atttaggatc gtcttttaca
     8761 acacataaaa ctaaatcgcc ttttgaatat gatatatgat taatatcttt ttgggcataa
     8821 cgatctagga gtattaagcg cgaaacactc gtaaagcttt tctccatatc atcatcgact
     8881 aaaaataaat gagggaaata gttttttaaa tcctcattta acttttgttt taactctttt
     8941 gtgtacttta cattttcttg cataatctca cataccttcc tacttaaatt ttatacttac
     9001 cttatttata gtattatatc acactaggac caataattcg agaaaaattt gtaaaatttt
     9061 ctttttcttt ttttcctgaa aaagcagttt ctgttttcaa aagaaaatag tttttactaa
     9121 agctttcaaa aaatcatttt atactacaat ttttcattgt tttttggtat aatatgatta
     9181 aatgggagat gttgcaatga ttcaaggatc taatcgtaaa atgagattat caaacagtgc
     9241 tacatactta caagacatct tttctaatac tccatatatt gaacttggta atattgatta
     9301 tgaaattatg gaaaagatct tgaattttga aagaattaga attgaggaaa tctcggattt
     9361 taatattcaa aatagtggta tgtctaactt taagattaat cgcgaaagtt tacaaaatat
     9421 aattccatat atgtttatcg atagtttgaa taagaagaat agagtttatc aacttttcct
     9481 aacttatggt ttggttcgtt atcgtgataa caataaagaa gaaaaatttg ctccaattgt
     9541 tttaattcct gtaaatttat attttgagaa taatcaaatt tatcttcaaa aatatgcacg
     9601 tccgattgca aatgatattt taattaatat tctttcaaca atcctaactt cagatcaaag
     9661 aagactatta ccaacacaag tattagatcg ttttgattct ttatattcaa tagatcgtta
     9721 tatcttacaa ctagccaata ttgaaggttt agatgtcaag ttagaaaact atctaacaat
     9781 tgccaagaca gttgcagata gaccagactt aaagcatagc aagtttccta ttgtaagaga
     9841 tcaaaatgaa gccttacttg ataagttata tagtaaagaa tgcaatttaa gatatatagc
     9901 tcctttaaat aaaaaacaaa gagaagcttt gtataatgca agtataggca atagttttgt
     9961 tattgtaggt agattaggta ctggtaaaac tttgacccta acaaacattt gtttagaagc
    10021 aattactcaa ggaaaaagaa ttctg
//
LOCUS       DAOSGS010000022         9809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_5898_length_9809_cov_4.652245, whole genome shotgun sequence.
ACCESSION   DAOSGS010000022 DAOSGS010000000
VERSION     DAOSGS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5898_length_9809_cov_4.652245"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..631)
                     /gene="rplL"
                     /locus_tag="PLQ22_01725"
     CDS             complement(260..631)
                     /gene="rplL"
                     /locus_tag="PLQ22_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOH58712.1"
                     /translation="MAKFNKDEFLKQLEEMSVLELNELVKAIEEKFGVTAAAAVVAGP
                     AAGPAEEEGPSVVNVILKAFGPNKIQVIKVVREITGLGLKEAKDLVDAAPKPVKEGIA
                     PEEAEGIKAKLIEAGAEVEIK"
     gene            complement(643..1137)
                     /gene="rplJ"
                     /locus_tag="PLQ22_01730"
     CDS             complement(643..1137)
                     /gene="rplJ"
                     /locus_tag="PLQ22_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH58713.1"
                     /translation="MKEQTLAKKVALVDKLVDEMHNAKSIVVVDYMGLTVEQMTDLRV
                     KLHAEKAKIHVIKNNISRRATEKLGYEKLGKYFVGPSAIIFSDDSTAAPRVACKYAKD
                     NKNLIIKAGVVDGNFRTDEELQVLATLPDKNGMLSMLLSVLEAPMRNLAFVIKQVAEQ
                     KEAN"
     misc_feature    complement(1148..1275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1306..2013)
                     /gene="rplA"
                     /locus_tag="PLQ22_01735"
     CDS             complement(1306..2013)
                     /gene="rplA"
                     /locus_tag="PLQ22_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOH58714.1"
                     /translation="MAKRSRKYQEMLSLVDRTKQYDLAEAIALAKKTHYAKFDATVEL
                     TFRLNLDPRQAEQNLRGAIVLPHGSGKSQSVLVLAQGDHLKAAEEAGADYFGNEEYLE
                     KIKNGWLDFDVLVATPDMMKDLGRFGKLLGPRGLMPNAKTGTLTMDIGRAVKEIKAGK
                     VEYRVDKNGNIPFSVGKISFEEQKLAENIKAVYELMVRSRPSTVKGVYVKNVALSTTM
                     GPGIKISPESIVAREAK"
     gene            complement(2091..2519)
                     /gene="rplK"
                     /locus_tag="PLQ22_01740"
     CDS             complement(2091..2519)
                     /gene="rplK"
                     /locus_tag="PLQ22_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOH58715.1"
                     /translation="MARKKVSTVVKLQLPAGRANPAPPVGPTLGQTGINIAQFCSQFN
                     DKTKEQMGNIIPTVITVYEDRSFSFITKTPPASDLLRKAAGIQKGSGKPNTVKAGKIT
                     EAQLREIATTKLPDLNAYTVEEAMKIVEGTARNMGIVVEK"
     gene            complement(2704..3369)
                     /locus_tag="PLQ22_01745"
     CDS             complement(2704..3369)
                     /locus_tag="PLQ22_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH58716.1"
                     /translation="MANELNELKDYVNEEGRDINVIEKQIPVKVGWGSTLFEVLLWVF
                     GIIPGLIFLFKKIQAKKFFDQLQQKIQHNASQIDNYLEQRVQVLQNVVGLVERSIDLD
                     KDVMKTVAAFRGGIHPTEENRSQVASQLESAIGSINVAFERYPDLKAHATIADAMRKN
                     DYLQREITAAREVYNDTVLRWNQEIFVWPTKKIVAAKNGYTTRIPFSVSKAVKEQARQ
                     KFF"
     gene            complement(3372..5126)
                     /locus_tag="PLQ22_01750"
     CDS             complement(3372..5126)
                     /locus_tag="PLQ22_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58717.1"
                     /translation="MDKEFIYKPVDEYKEYARLHHENISQYFDELTKTANTDVGANEL
                     TVKKIRTLEKENDGIHKQQSRYQMLKVFLWILVAIAIFVFFYAIYLTASDAAVETVSL
                     VLMFIISAIVIVGGLLLIYKVIKPKYLVLEAKSQELEREISNQYKVAYQQMGSLNALF
                     KVEVIAELFEKTIPLLDFDKMFSQKTLEFLKTTFGFDDSTSEEQSTLYVQSGHLNGNP
                     FALFKNLYHVLGTKTYVGTKTISWTETIGSGKDRRTVTRTQTLTATLEKPCPYYSEQT
                     HLVFASEVAPDLTFYRKQGYTHRLTPKQVERKVRSDMEDFREDTEDNFSKSITLLAND
                     EFEVLFRALNRNNEVQFRLLFTPLAQQNMIDLLLDKTEGFGDDFAFAKKNKLNFISPD
                     HMQGFKLDITPKHFVDYDYNRCKQNWINYNMEYFRKMYFTFAPILCIPLYQQHPPHEY
                     VYRDVVESYSSFFEDEMIVNNLGYAKFSHPDSKTRNILKTKLVNINGKVEDIQVTAYG
                     YNVEKRVTYIPKTGGDGRVHQVPVHWDEYLPVVQTTDIVMDFFDDLDKIQEMMRNQDF
                     QGVLDRNMCLVKFRGKEE"
     gene            5244..5699
                     /locus_tag="PLQ22_01755"
     CDS             5244..5699
                     /locus_tag="PLQ22_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58718.1"
                     /translation="MKQVVKFSNLIAAILGALAIVFLAITPGMKLNVPLETEGFSGFQ
                     AIFGYSETIMGHSVEVLAPNWVGIIAFVLLAVGVIAGVIGMVIGNKMANLVSAVVLLA
                     GGILLFLVPTIANGEHETFKLSTWLIISGIIAILGGLFGCFGIFVKSKK"
     gene            complement(5776..6330)
                     /gene="nusG"
                     /locus_tag="PLQ22_01760"
     CDS             complement(5776..6330)
                     /gene="nusG"
                     /locus_tag="PLQ22_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOH58719.1"
                     /translation="MDLQEPRAWYIVQTVSKHEEQAKMNIELRVESLGMQEYIFNVLV
                     PKKIVREKKKNGEYKEVEVILYPKYIFIDMIVTDDTWFMVRNTPLVTGFLGSSGGGAK
                     PVPVSLEEMQTIFDLMGIKEEDKTEFEGQIGDQVEIISGNFKGSEGVIESIDLSKKIA
                     VVLISMFGRATPAELSFSDLKVKK"
     gene            complement(6330..6869)
                     /gene="secE"
                     /locus_tag="PLQ22_01765"
     CDS             complement(6330..6869)
                     /gene="secE"
                     /locus_tag="PLQ22_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOH58720.1"
                     /translation="MSQNKDLKAQEKQALKEEKQAKKLKEKEEKKKLKEAVKEKAKKK
                     HGAASVFSKEYKYEGLILLILSIISLVLGTLVLLGHSGKDGLTIPDYVWLIGTYPKLM
                     AWILIGLAVSGLILSVIPLFKPSIEEFKRVTWLTGGQLLKNTGIVLTFVIIMALFFFV
                     ADLGLAQVIKFFDWAAGKM"
     gene            complement(6882..7031)
                     /gene="rpmG"
                     /locus_tag="PLQ22_01770"
     CDS             complement(6882..7031)
                     /gene="rpmG"
                     /locus_tag="PLQ22_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH58721.1"
                     /translation="MRVKVTLICEECLSRNYQVTKNKINSPERIAIKKYCPKCNKHTI
                     HKETK"
     gene            complement(7095..7634)
                     /locus_tag="PLQ22_01775"
     CDS             complement(7095..7634)
                     /locus_tag="PLQ22_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH58722.1"
                     /translation="MIYNYNDYELLYLIREKSDEALEIMYRKYTPLIRKRILDFRIQV
                     NHEDFFQEGLLALDIAIRRYIDLYDKTFNKYFDLILQRRFIQILRKQEKHYYNVYNLE
                     SPEMLYEETEQYDYTPVDLPLGSLSEFEKEVFETFKTKALKPKEVAKILNVDIRSVYN
                     AFSRIKQKLQNVKENKDNK"
     gene            complement(7662..8366)
                     /gene="rlmB"
                     /locus_tag="PLQ22_01780"
     CDS             complement(7662..8366)
                     /gene="rlmB"
                     /locus_tag="PLQ22_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOH58723.1"
                     /translation="MSELVYGKNSVREILKSERKVLKLYLVKEDQEIESLAKAKKIAI
                     EYSLKEEISRKIKGNHQGYLAIVEEYQYYDLSIVDSFNSKIPFVVILDSITDPHNLGA
                     IIRTAEAAKVDAVIIPKNRSAEVNSTVTKTSTGATEHVRIIRVNNLNQTIKVLKEKGY
                     WIVGTENTDKSILYTELKYDFPVALVIGSEGKGISQLVAKNCDYLIKIPMKGKINSLN
                     ASVSAGILIYEIIRNR"
     gene            complement(8359..8742)
                     /locus_tag="PLQ22_01785"
     CDS             complement(8359..8742)
                     /locus_tag="PLQ22_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOH58724.1"
                     /translation="MDYDLLNGGNLAYIGDAYFTLRIREYLLGKGITEMNRLQKMSVE
                     YVSAKAQANVVLKLLPNLTEEETLVYKRGRNYKTRAARKIDIKDHNISSGFEALIGYL
                     YLSKQNERLEEIIQLTIRVVEEKNE"
     gene            complement(8742..>9809)
                     /locus_tag="PLQ22_01790"
     CDS             complement(8742..>9809)
                     /locus_tag="PLQ22_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOH58725.1"
                     /translation="KLAYNTYKKLNIRPHDYNPRVTENMENIVNFIKLLKDKGYAYEA
                     GDDIYFSVGKIANYGILSGQAQDKLQIGARIDVDDKKEDPADFTLWKKTNEGITWCAP
                     WGDGRPGWHTECVVMIDSIFKGPIDIHGGGMELKFPHHDNEIAQAEAAYNHRIANYWM
                     HNGRINMQGTKMSKSLGNIVWADDLLDEVGYGPFRILILTTHYRQPLEYSDPILKQAM
                     TDYERIYRTYFQLFRKVELANGSKREVKDKELLEIRSKFQDALKNDFNTPNAITEIFT
                     LVKVVNTALRDKTTNLEFLEMALSLFDELLDVLGLNIEMKKLTIEEKELVNKWQDARN
                     KKDFEKADKLRAEMNERGIIV"
BASE COUNT         3034 a         1811 c         1204 g         3760 t
ORIGIN      
        1 ttcattatta caagtgacaa aatcctcatc aattggacaa aacttaaata aataataaac
       61 tgcaattaaa gtattaccta tttcaaaatg catatctaaa ggatactctt tacacttctt
      121 acaatcaaaa acttctaaca aattatataa tgatctttct tttttacact ccatattccc
      181 tcctaactat atattattag tcaagagaat aaatcttttt aataaaataa aaagtgctag
      241 aaaactagca cttttttgac tatttaattt ccacttcagc tcctgcttca attaacttag
      301 ctttgatacc ttcagcttct tcaggtgcaa taccttcttt aactggtttt ggtgcagcat
      361 ctactaaatc tttagcttct tttaatccta agccagtaat ttctctaact actttgataa
      421 cttgaatttt gtttggacca aaagccttta aaataacatt tactacgctt gggccttctt
      481 cttcagctgg accagcagct ggacctgcaa ctacagcagc agcggcagta actccgaatt
      541 tttcttcaat tgcttttact agttcgttaa gttctagtac gctcatttct tctaattgtt
      601 ttaagaattc atctttatta aatttagcca tttttttcct ccttaatttg cttctttttg
      661 ttctgcaact tgcttaataa caaaagctaa attgcgcatt ggtgcttcta gcacgcttag
      721 taacattgat agcataccat ttttatctgg taatgttgcc aatacttgta attcttcatc
      781 tgttctgaag ttgccatcta caactcctgc tttgataatt aaatttttat tatcttttgc
      841 atacttacat gcaactcttg gtgcagcagt tgaatcatca gaaaaaatga tagccgatgg
      901 ccctacaaag tattttccta atttctcata acctaatttt tcagttgctc ttcttgaaat
      961 gttgtttttg ataacatgaa tttttgcttt ttcagcatgt agtttaactc ttaaatcagt
     1021 catttgttca actgttaagc ccatataatc tacaactaca attgatttag cattatgcat
     1081 ttcatcaact aacttgtcaa ctaaagcaac ttttttcgct agagtttgtt ctttcatttt
     1141 ttcctccatt tcaaataaaa aacctcattt tttccgcatg taaaaagagg ttcgtttttc
     1201 ttttcacctc ggcaggcaat tttaagctta agataactta agcacccgag tctacggcat
     1261 agtttaaact attttattat actattttaa tttttgtttt taattttatt ttgcttctct
     1321 tgcaacaatt gattctggac taattttaat tccaggaccc attgtcgtag atagtgcaac
     1381 gtttttcaca taaacacctt taactgttga tggtcttgat ctaaccataa gctcataaac
     1441 agccttgatg ttttcagcta atttttgttc ttcgaaactt attttcccaa cactaaatgg
     1501 aatatttcca tttttatcta cacgatattc aactttacca gccttaattt ctttaactgc
     1561 tcttccaata tccatagtaa gagttccagt cttagcatta ggcattaatc ctcttggtcc
     1621 taaaagctta ccaaaacgac ctaagtcttt catcatgtct ggtgttgcta ctaatacatc
     1681 aaagtctagc catccgtttt taattttttc taaatactct tcatttccaa aataatctgc
     1741 tccagcttcc tcagctgctt ttagatgatc accttgagct aatactaaaa cactttgtga
     1801 tttaccacta ccatgaggta gtacgattgc accacgtaag ttttgttcag cttgtcttgg
     1861 gtctaagttt aatctaaaag ttaattcaac tgtggcatca aattttgcat aatgagtttt
     1921 ctttgcaagc gcaatagctt cagctaaatc atattgtttt gtacgatcaa ccaagcttag
     1981 catttcttga tatttacgac ttctctttgc catatttgtt tacctcctta aatgtggttt
     2041 agcggtcatc ctcccacttt ttaagttatt taacttaaag ggaaaatcat ttacttttca
     2101 acaacaattc ccatatttct tgcagttcct tcaacaatct tcattgcttc ctcaactgta
     2161 taagcattta aatccggtaa tttagtagtt gcaatttctc ttaattgcgc ttcagtaatt
     2221 ttaccagcct ttacagtatt tggttttcca gaaccttttt gaataccagc agcttttctt
     2281 aataaatctg atgctggtgg agttttggtg atgaaagaaa atgaacgatc ttcgtataca
     2341 gtaataacag ttggaataat gttacccatt tgctctttag tcttgtcatt aaattgtgaa
     2401 cagaattgag caatgttaat accagtttgt cctaatgtcg gtcctactgg tggtgctggg
     2461 ttagctcttc cagctggtaa ctgtagttta acgactgtcg aaactttttt tcttgccacg
     2521 agacacacct cctttttttg tccgtgatgt ggttcgatta atatctccca cttcatcaat
     2581 aaaatagtat gcgtactatg cacgcttaag tattatacaa caattaatca aaaaagtcaa
     2641 cgaaattatt cgcttaaaat caaaataaac ctataaaaaa taagcctttt taaggcttat
     2701 tttctaaaag aatttttgtc ttgcttgttc ttttacagct ttacttactg agaatgggat
     2761 tctcgtagta tatccatttt ttgctgctac aattttcttt gtaggccaaa caaaaatttc
     2821 ttggttccat cttaaaactg tgtcgttata aacttctctt gcagctgtaa tttctctttg
     2881 taaatagtca ttttttctca tcgcatcggc aatagtagcg tgtgctttta aatccggata
     2941 tctttcaaat gccacattaa tgctaccaat agctgattca agttgtgatg caacttgact
     3001 acggttttct tcagttggat gaattccacc tctaaaagct gctacagttt tcataacatc
     3061 tttgtctaaa tcaattgatc tctccacaag tccaactacg ttttgtaata cttgaactct
     3121 ttgttctaag tagttatcga tttgcgatgc attatgttgg attttttgtt gtagttgatc
     3181 aaagaatttc tttgcttgaa ttttcttaaa caagaaaatt agacctggaa taataccaaa
     3241 aacccataat aatacttcaa aaagagttga tccccagcct acttttactg gtatttgttt
     3301 ttcaattaca ttaatgtcac gtccttcttc attgacataa tcttttaatt cgtttaattc
     3361 attagccatt tttattcctc cttgcctcta aatttaacta aacacatatt tcgatctaaa
     3421 actccttgaa aatcttgatt tctcatcatt tcttggattt tatctaaatc gtcaaaaaag
     3481 tccattacaa tatcagtagt ttgtacaacc ggtaaatatt catcccaatg aactggaact
     3541 tggtgaactc taccatctcc accagttttg gggatataag taactctttt ctctacgtta
     3601 tatccataag cagttacttg aatatcctca actttaccat tgatattgac taattttgtt
     3661 tttaaaatat tccttgtttt tgaatctgga tgactaaatt tagcatatcc tagattattt
     3721 acaatcattt catcttcaaa aaacgaagaa taactctcta caacatccct ataaacatat
     3781 tcatgtggtg gatgttgctg atataaagga atacaaagaa ttggtgcaaa agtaaaatac
     3841 attttcctaa aatattccat attataatta atccaatttt gtttacacct attataatca
     3901 tagtcgacaa aatgtttagg tgttatatca agtttaaatc cttgcatatg atcaggcgaa
     3961 ataaagttaa gtttattttt cttggcaaat gcaaaatcat cgccaaagcc ttcagtctta
     4021 tcaagcaata aatcaatcat attttgttgt gccaaaggtg taaataataa tctaaactga
     4081 acttcattat tacgatttaa agctctaaat aatacttcaa attcatcatt agcaagtaaa
     4141 gtaatagatt ttgagaaatt atcttcagta tcttctctaa aatcttccat atcagatcta
     4201 acttttcttt caacttgctt aggagttaaa cgatgagtat aaccttgttt acgataaaat
     4261 gttaaatcgg gtgcaacttc agaagcaaaa actaaatgtg tttgttcact atagtaagga
     4321 caaggttttt ctaaagtcgc agttaatgtt tgtgttctcg taactgtcct tcgatctttt
     4381 cctgacccta tcgtttctgt ccaagaaatg gtttttgtac ctacataagt ttttgtacct
     4441 aaaacatggt ataaattctt aaataaagca aaaggattac catttaaatg ccctgattga
     4501 acatataaag ttgattgttc ttcagatgtt gaatcatcaa atccaaatgt tgtttttaaa
     4561 aactctagtg ttttttgtga aaacatctta tcaaagtcta gcaaaggaat agttttttca
     4621 aaaagttctg ctattacttc aactttaaac aaagcattta aactacccat ttgttgatag
     4681 gcaactttat attgattaga tatttctctt tccaattctt gactttttgc ttccaacact
     4741 aaatatttag gttttataac tttatatatt aataaaagtc cacctacaat tacaattgct
     4801 gaaataatga acattaatac aagtgataca gtttcaactg ctgcatcact tgcagtcaaa
     4861 tatattgcat aaaaaaagac aaaaatagct attgcaacca aaatccaaag aaaaactttt
     4921 aacatttgat aacgtgattg ttgtttgtgt ataccatcgt tttctttttc taaagttcta
     4981 attttcttta ccgttagttc attagctcca acatccgtat tagcagtttt agttaattca
     5041 tcaaaatatt gactaatatt ttcgtgatga agacgtgcat attccttata ttcatcaacg
     5101 ggtttataaa taaattcttt gtccatttat ttctcctatg cttaaattat atcatatatt
     5161 gatttgtaaa acaataatac tcgcttcttt tttgcttgtt ttttaccatt gactatgata
     5221 tactcttacc gaggaggaaa tgtatgaaac aagttgtaaa attttcaaat ttaattgctg
     5281 ctattttagg cgctttagct atcgtattcc tagcaattac tccaggaatg aaattaaatg
     5341 ttccacttga aacagaagga ttcagtggct tccaagctat ttttggatat tctgaaacaa
     5401 ttatgggaca ctctgttgaa gttcttgcac ctaactgggt tggtattatc gctttcgttt
     5461 tacttgcagt tggtgttatc gctggtgtta ttggcatggt tatcggtaac aaaatggcta
     5521 atttagtgtc tgctgttgtt ttacttgctg gcggtatttt attgttcttg gttccaacta
     5581 ttgcaaatgg tgaacatgaa actttcaaac tatctacttg gttaatcatc agtggtatca
     5641 ttgcaatttt aggcggatta tttggttgtt ttggtatttt tgttaagagc aaaaaataat
     5701 aaatacatcc taagctcctc atataggagc ttttttaatg ccctaaaaat aaaaaataag
     5761 ccctttcaaa ggggcttatt tttttacttt taaatctgaa aaactaagtt cagctggtgt
     5821 agctcttcca aacatactta ttaaaactac agcaattttc tttgataagt ctatactttc
     5881 aataacacct tcagaacctt taaaattacc tgatataatt tcaacttgat caccaatttg
     5941 tccttcaaat tcagttttat cttcttcttt gatacccatt aagtcaaaga ttgtttgcat
     6001 ctcttctaaa ctaacaggaa ctggtttagc acctccacca cttgatccta agaatcctgt
     6061 aactagcggt gtgtttctaa ccataaacca agtatcatca gtaacaatca tatcaataaa
     6121 aatatattta ggataaagaa ttacttcaac ttctttatat tctccgtttt ttttcttttc
     6181 acgaactatt tttttaggaa ctaaaacatt aaaaatatat tcttgcattc ctaaagattc
     6241 aactcttagt tctatgttca tcttagcctg ttcttcatgt ttagaaacag tttgaacaat
     6301 gtaccaagct cttggttctt gtaaatccat tacattttcc ccgcagccca gtcaaagaat
     6361 ttaataactt gagctaagcc aagatcagct acaaagaaaa ataaagccat aataattacg
     6421 aacgttaaaa caataccagt gttctttaat aattggccac cagttaacca agtaactctt
     6481 ttaaactctt caattgatgg tttaaataat gggataactg ataaaatcaa accactaact
     6541 gcaagaccaa ttaagatcca agccattagt tttggataag taccaattaa ccaaacataa
     6601 tcaggaattg ttaatccgtc tttgccagaa tggccaagta aaactaatgt tcctaaaact
     6661 aaagaaataa ttgataaaat taaaagaatt aaaccttcat atttatattc tttactaaat
     6721 actgaagcag caccatgctt tttctttgct ttttctttta ccgcttcttt taatttcttt
     6781 ttttcttctt tttctttcaa ttttttagct tgtttttctt cttttaaagc ttgcttttct
     6841 tgtgctttta aatctttatt ttgagacatc taaatctcct cctatttcgt ttctttgtga
     6901 atcgtatgtt tattgcattt aggacaatac ttcttaatag cgattctttc tggagaatta
     6961 attttgtttt tagtaacctg ataatttcta gataaacatt cttcacaaat taatgttact
     7021 ttaactctca tttaaatcct tctcctcata aatatactac aatataatat caaatttcac
     7081 caaaattgtc aagattattt attatcctta ttctctttta cattttgaag tttttgctta
     7141 atcctagaaa atgcattata aacactccta atatcaacat tcaatatttt cgctacctct
     7201 tttggtttca aagctttagt cttaaaagtc tcaaaaactt ctttttcaaa ctcgcttaaa
     7261 gatcctaatg gaagatcaac tggagtataa tcatactgct ctgtttcttc atataacatc
     7321 tctggtgact ctaaattata aacattgtaa taatgttttt cttgttttct taatatttga
     7381 ataaatcttc tttgtaagat taaatcaaaa tacttattaa acgtcttatc atataaatca
     7441 atataacgtc taatcgcaat atctaatgct agtaaacctt cttgaaagaa atcttcatgg
     7501 tttacttgaa tcctgaaatc taaaattctc ttcctaatca aaggtgtata cttacgatac
     7561 ataatttcca aagcctcgtc agacttttct cgaatcaaat ataataattc gtagtcgtta
     7621 tagttataaa tcataactac acctcctttg aactattaaa tttaacgatt tctaattatt
     7681 tcataaataa gtattcccgc actaacagat gcgtttaaag agtttatttt tcctttcatt
     7741 ggtattttta ttaaataatc acagttttta gctacaagct gtgatatacc ttttccttca
     7801 ctgccaataa ctaaggcaac aggaaaatca tattttaatt ctgtatataa aatactttta
     7861 tcagtatttt ctgtcccaac aatccagtat cctttttctt ttaatacttt aatcgtttga
     7921 tttaaattat ttaccctaat aattctaaca tgttcagtag ccccagttga tgttttggta
     7981 actgtgctat taacttctgc tgatctattt ttaggaataa taacagcatc tacttttgca
     8041 gcctcagctg ttctaataat tgcccctaaa ttatggggat cagtaatact atctaaaata
     8101 accacaaaag gaattttaga attaaaacta tcaacaatac tcaaatcata gtattgatac
     8161 tcttctacaa tcgctaggta gccttgatgg ttacctttta ttttgcgtga aatctcttct
     8221 tttaaactat actcaatggc aattttttta gcttttgcca agctttcaat ttcttgatct
     8281 tcttttacta aataaagttt taaaacctta cgttcagatt ttaaaatctc acggactgaa
     8341 ttctttccat aaactaactc actcattttt ttcctctaca actctaatag ttaattgtat
     8401 tatctcttct aatctttcat tttgctttga taaatacaaa taaccaatta atgcctcaaa
     8461 accagaacta atattatgat ctttaatatc aatctttctt gcggcccttg ttttatagtt
     8521 acgtcctctt ttataaacta aagtttcttc ttcagttaaa ttaggtaata actttaaaac
     8581 aacatttgct tgagctttag cactaacata ttcaacactc attttttgca acctgttcat
     8641 ttcggtaata ccttttccaa gtaaatattc cctaattctt aaagtaaaat aagcatctcc
     8701 aatataagct aaattgccac catttaataa atcataatcc attaaactat gattcctcgt
     8761 tcattcattt ctgctcttaa tttgtctgct ttttcaaaat ctttcttatt acgagcatct
     8821 tgccatttgt taactaattc tttttcttca atagttaact ttttcatttc aatatttaaa
     8881 cctaaaacat ctaatagctc atcaaataaa cttaaagcca tttctagaaa ctctaaatta
     8941 gtagttttat ctcttaaagc agtattaact acttttacta aagtaaagat ttctgtaatt
     9001 gcattaggtg tattaaaatc atttttaaga gcatcttgaa atttacttct aatttctaat
     9061 aactctttat cttttacttc cctttttgaa ccatttgcta gttctacttt tctaaacagt
     9121 tgaaaataag ttcgataaat tctttcataa tcagtcatag cttgttttag aattggatca
     9181 ctatattcta aaggttgacg ataatgagtt gttagaatta aaattctaaa tggtccgtaa
     9241 cctacttcat caagtaggtc atcagcccaa actatatttc ctaaagattt actcatcttt
     9301 gttccttgca tattgatacg accattgtgc atccaataat ttgcaatacg atgattataa
     9361 gcagcttcag cttgagctat ttcattatcg tggtgtggga attttaattc cattccgccc
     9421 ccatgaatat caataggccc tttaaaaata ctatcaatca taacaacaca ttcagtatgc
     9481 catccaggtc ttccatcgcc ccatggagca caccaagtaa taccttcatt tgtttttttc
     9541 cataaagtaa agtctgctgg atcttctttt ttatcatcaa catcaatcct agcgccaatt
     9601 tgaagtttat cttgtgcttg gccacttaaa atcccataat tagcaatttt accaacagaa
     9661 aaataaatat catctccagc ctcataagca taacctttat cttttaaaag tttaataaag
     9721 ttaacgatgt tctccatatt ttctgtaact cttggattat aatcatgtgg tctaatgttt
     9781 aactttttat aagtgttata tgctaactt
//
LOCUS       DAOSGS010000023         9638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6024_length_9638_cov_5.180215, whole genome shotgun sequence.
ACCESSION   DAOSGS010000023 DAOSGS010000000
VERSION     DAOSGS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6024_length_9638_cov_5.180215"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLQ22_01795"
     CDS             complement(<1..519)
                     /locus_tag="PLQ22_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58726.1"
                     /translation="MKIIFNLILAFFLSLTTPVVTAKAEYQADVNKGYDSYSYIVDYE
                     NSYYSLIVVEGLVDDKITYGVFFFNDVSRAYYITLVNEQTGLRQLFPETDRGDVYVLG
                     FQVEKGSSYTILIRDKKSHEQHLPFELSFGLKTEEEISSLATSGLGNGVSMTDLETAY
                     WFKPFKINLPALI"
     gene            complement(503..1333)
                     /gene="prmC"
                     /locus_tag="PLQ22_01800"
     CDS             complement(503..1333)
                     /gene="prmC"
                     /locus_tag="PLQ22_01800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOH58727.1"
                     /translation="MTYQELFKKYTNQLNKDQERTAIELIVLEVNGLSKQDLILNYNN
                     QVLHLKKTETLISEYLQGMPVQYLLGFTYFYGYKFFVSPAVLIPRFDSEILIEAVLEH
                     LDKGQEYRILDIGTGSGNLAITLKKLLPKAIIEAVDISDSALELAKKNAKYHKVDINF
                     IQSDLFSNVNNKYDIIISNPPYIRKEDLLSEYVLKEPKGALIAEEDGLYYYKTILKEI
                     NRYLNDKYLVFFEIGINQETKVTEYVRKYLGAKTKVYLDLNLIPRVVMIEGDKSENNI
                     "
     gene            complement(1333..2400)
                     /gene="prfA"
                     /locus_tag="PLQ22_01805"
     CDS             complement(1333..2400)
                     /gene="prfA"
                     /locus_tag="PLQ22_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH58728.1"
                     /translation="MLERLQIIEDRYEEICNLLMQPEIAMDLKKVKELSKEQKRLEPI
                     VTKSRSYKSLLEEEKSLESLITGHDLEIAELAKLEIEGVKEEILKLEEELKLMLIPRD
                     PQDDKNIICEIRGAAGGDEANIFAGDLFRMYSKYAEEKGWTIEVINSEPSDVGGFSKI
                     EFIITGDSVYSFLKYESGAHRVQRVPETEASGRIHTSTATVLVMPEVDEVEVNLDVND
                     VRIDTYRSGGAGGQHVNKTESAIRLTHIPSGIVVQCQDGRSQHENKATAFKLLAARLY
                     EHALQEQEQQVGRERLAKVGTGDRSEKIRTYNYPQNRVTDHRINFTIQQLDRVMNGKL
                     DPIIEALIMENQRAQLSKKEI"
     gene            complement(2400..3728)
                     /gene="dnaB"
                     /locus_tag="PLQ22_01810"
     CDS             complement(2400..3728)
                     /gene="dnaB"
                     /locus_tag="PLQ22_01810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOH58729.1"
                     /translation="MEELRQLPYSIESEQYILASALINPQEASAIVNSVYSEHFYDQR
                     HKNIMEAMEKLYQNHQEINYTNVIEELKREKKFEASGGIEYIGYLIDILPTYVRWDTY
                     AANIKEKALQRELYHTLSNLSNDILSGRFQTDDILNNTERLVKNVLNKRSVNQLTKVD
                     SITPGVIDEINKRMQNKNDLIGIDTGFKELNNILSGFKKQELIILAARPGVGKTALAL
                     NFASAACKQGKYVAFISLEMGKEQIVKRLISTESAVDHDKINKGQLSGTEISSILTAK
                     SQTIDKFNLYLDDSNSANIFDIMAQCRSLKRNGKLDMIVVDYLQLIDLDNPRRSSTRA
                     EIVGAISRSLKVLAMELDIPIIALSQLNRTAAKPDGEPNLAQLRESGSIEQDADVVMF
                     IYSNKEDEERNQKTANLKLKVEKNRHGRTGIMDITFFKHILKFTQEKEQR"
     gene            complement(3731..6169)
                     /gene="rplI"
                     /locus_tag="PLQ22_01815"
     CDS             complement(3731..6169)
                     /gene="rplI"
                     /locus_tag="PLQ22_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOH58730.1"
                     /translation="MRNTLVYRIISLVLFLASCFVLVFTAIFGEINEWLTYAALVIVA
                     ASFTTVFVLNVGNKKFRKMQWLEQRYELWNSISYRVKKGGETAFNKLPIAIIVYDKNK
                     TIQWANAYAKEIFLSPLVDVRIENISADLYYNLSNEDKFEISLYGSIFECEVLMEENI
                     VYLTDVTEFHNLTQKYADHTLAAGIINLDNLSEALSGFDAQERSSQMSNIIGILSDWC
                     SKFDIYLKGYTDNQYLILLNEKQLAEVVKDEFKVIDDVKSYCLKEDIRSSASIGIAVA
                     DNADLGYVELFALAETQLNLALNRGGNQCVLNKYGETSYFGGKTASFESRSPVYVRTK
                     AEELVSLINKAQKIYIMTHIDADADAFGAAIAVQKIAAAYNRDAKIVFDLDKVDQTIQ
                     YLYSIIQREHTATLDYLVGSKKAIRDIKASDLLIIVDVQNQKILMEPKLYKKAKHVAV
                     IDHHRPGKFLVDKYDYIYQTSSASSSVELLVEMYEFLDKDPIVSSAEATWMIMGILVD
                     TNDLMYRASYRTFNVLSRLQTYGGELQLAKKYLREDYDKYEKRIDVLSNLIIYKEKYG
                     IATVTEGVNPRQFLAKVADDVLEIKGIKASFCVGRISENEIGISARSLDETNVQLIME
                     ALGGGGHYNNAATQLRAVDLEEAKDMLIKEIDISEERGGDSMRIILTTEVKGKGKKGD
                     IIDVKSGYGNFLIRSGQAIIASVDNIKELEKEKEKEKRLALSKENEMKDLRDKIEANP
                     VTIGVKVGKEGKIFGSVSSKMIVDEYKAQYDITLDRKKIIKDEIQALGTFVVLVDLHP
                     DIKARLTIYVVGKE"
     gene            complement(6261..6482)
                     /gene="rpsR"
                     /locus_tag="PLQ22_01820"
     CDS             complement(6261..6482)
                     /gene="rpsR"
                     /locus_tag="PLQ22_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOH58731.1"
                     /translation="MAFRPRRRKSCYFTDNKIEKIDWTDYELLRRFVTDRGKIKPRRS
                     TGTKAIYQRQLAVAIKRARHMALLPFVKE"
     gene            complement(6496..6960)
                     /gene="ssb"
                     /locus_tag="PLQ22_01825"
     CDS             complement(6496..6960)
                     /gene="ssb"
                     /locus_tag="PLQ22_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH58732.1"
                     /translation="MMNKVVLTGRLTKDPELKKTNSGISYCFFTIAVNRSYVNRQGNR
                     EADFINCVAWRVQAENLARYMRKGSLIGVDGEVRTRSYQDQDNKMVYITEILANAIEF
                     LESKSAGSSQGYQSFSQDSPFEFNQKPEPRKQEDPFKEMQAGLGITDDDIPF"
     gene            complement(6960..7241)
                     /gene="rpsF"
                     /locus_tag="PLQ22_01830"
     CDS             complement(6960..7241)
                     /gene="rpsF"
                     /locus_tag="PLQ22_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH58733.1"
                     /translation="MREYTGMYIIHPEHTEEEFKAIVSEIAKIFEEHGSRVLEIDEWG
                     MKDLAYEINDLKKGYYVKFRVEATPEAVSEYNRICNIREDIIRHILVRD"
     gene            complement(7408..>9638)
                     /locus_tag="PLQ22_01835"
     CDS             complement(7408..>9638)
                     /locus_tag="PLQ22_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOH58734.1"
                     /translation="EYTNLLFLGTVLSNMIIGIIQETRAKKQIDQLSLMFVPSASVIR
                     NGEQEDIPIDEVVLDDVLTFKSGKQIGCDCILKEGSLEVNESLITGESIAIHKKIGDT
                     LYSGSYVVGGVGVAIADKVGSASYVEKLATGAKKHKYAKSDILNSLNWILKIVSIIII
                     PLAVATYLNSYDSLPEINPNTGRAWEGFEKFAEAFKSTSGSVISMIPAGLFLLTTVAL
                     TVSVIRLAKKKVLVQEIYCIEMLARIDILCLDKTGTITDGTMKVIDSVQLETKGDYTI
                     REIIGSMMNAFPESNATSNALAEHYGKNSILKADGIIPFSSQRKYSAVRFGKLGYYII
                     GAPEIVLNETNYAKIQRRVERFAAQGCRVIVLAHTKSPINPTSRPKSTTALAVIAIQD
                     HIRDDAEQVIAYFKNNEVDIKVISGDNPITVAEIARRAGVSDASRYISLDGLSDEEVI
                     AAATEYNVFGRVSPYQKKLLVQTFKMNKKTVAMTGDGVNDILALKEADCSIAMASGSD
                     ATKHVSHIVLLESNFSAMPEIVAEGRRVTNNIQRTAILFLTKTLFSILLTIFYLAVRR
                     AYPFSPNQLFPFEWFVLALAATLLALQPNTEKIKGRFISNVLRNVLPGAFTVLLAHAI
                     IYFLTKIDGFAILSTNEGYSTVTLYVTTIIMFYVLYFVSRPLNFYRFLVFIVSAAGAV
                     LSILFLNEPLELGYVKLDLVGYLLVLVFGFGIYLVLDFIYWLLKKIRIIPKQETGERD
                     SI"
BASE COUNT         3152 a         1791 c         1145 g         3550 t
ORIGIN      
        1 aattaacgct ggtaaattga ttttaaaagg tttaaaccaa taagcagtct caagatctgt
       61 catagatacg ccatttccta aaccactagt agctaatgaa ctaatttctt cttctgtctt
      121 taaaccaaaa cttaactcaa aaggtaaatg ttgttcatgt gattttttat cacgaattaa
      181 aattgtataa cttgaacctt tttcaacttg aaaccctaaa acataaacat ctcctctatc
      241 tgtctcaggg aataattgtc ttaaaccagt ttgttcgttt actaaagtaa tatagtaagc
      301 cctacttaca tcattaaaaa agaaaacacc ataagttatc ttatcatcaa ctaaaccctc
      361 aactacaatt aagctataat aactattttc ataatctact atatatgaat aagaatcata
      421 tcccttattc acatcagctt gatattcagc tttagctgta actaccggag tagttaaact
      481 tagaaaaaaa gccaatatta aattaaatat tattttcact tttatcacct tctatcataa
      541 ctactctagg aattaagttt aaatctaaat aaactttagt tttagctcct aaatactttc
      601 taacatattc tgttacttta gtttcttgat ttattcctat ttcaaaaaaa actaaatact
      661 tatcatttag atatctatta atttctttca aaattgtttt gtaataataa agaccatcct
      721 cttcagctat taaagcacct tttggttctt tcaaaacata ttcacttaat aaatcctctt
      781 ttctaatata aggaggatta gaaataataa tatcatattt attattcaca ttactaaaca
      841 aatcgctttg gataaagttg atatctactt tatgatattt tgcatttttc ttagctaatt
      901 ctaaagcact atcacttata tctactgcct caattatagc cttaggtaat aattttttca
      961 aggtaattgc aagattccct gaaccagtac caatatctaa aattcggtat tcttgtcctt
     1021 tatctaaatg ctctaaaaca gcttcaatta aaatctcact gtcaaaacga ggaattaaaa
     1081 cagcaggact tacaaaaaat ttgtatccat aaaagtaagt aaaccctaat aaatactgaa
     1141 cgggcatacc ttgtaagtat tcactaatta gggtttctgt tttttttaga tgcaaaactt
     1201 gattattgta attaagaatt aagtcttgtt tagaaagtcc gttaacttct aaaacaatta
     1261 attcaattgc tgttcgttct tgatctttat ttaattgatt tgtatatttt ttaaataatt
     1321 cttgataggt cattatattt ctttttttga taattgggct ctttgatttt ccataattaa
     1381 agcctcaata atcggatcta attttccatt cattacacga tctaattgtt ggatggtgaa
     1441 gttaattcta tgatcagtta ctctattttg gggatagtta taagtcctaa ttttctcaga
     1501 acgatcacca gtaccaactt tcgctaatct ttctcttcca acttgttgtt cttgttcttg
     1561 aagagcatgt tcatataatc ttgcagctaa aagtttaaaa gctgtagctt tattttcatg
     1621 ttggcttcta ccatcttgac attgaacaac aattcctgat ggtatatgtg tcaaacgaat
     1681 agctgattct gttttattta catgttgtcc gccggctcca cctgaacgat aagtatcaat
     1741 tctaacatca tttacatcta agttaacttc aacttcatca acttcaggca taactaagac
     1801 agtcgcagtt gatgtgtgaa ttcttccgct tgcttctgtt tctggaactc tttgaacacg
     1861 atgagcacca gattcatatt ttagaaatga ataaacgcta tcaccagtaa ttataaattc
     1921 aattttagaa aatccaccta catctgaagg ttcagagtta ataacttcaa ttgtccaacc
     1981 cttttcctct gcatatttag aatacattct aaataaatca ccagcaaaaa tattagcttc
     2041 atctccacct gcagctcctc taatctcaca aatgatgttt ttatcatctt gaggatcacg
     2101 aggaataagc attaatttta actcttcttc caattttaag atttcttctt taacaccttc
     2161 aatctctaac ttagcaagtt ctgctatttc aagatcatgg ccagtaatta aactttctaa
     2221 agatttttct tcttctagta aagatttata acttcttgat ttagttacaa taggttctaa
     2281 tcttttttgt tctttactta attcttttac ctttttaaga tccattgcga tttctggttg
     2341 cattaataaa ttgcatatct cttcgtaacg gtcttcaata atttgcaacc tctcaagcat
     2401 tatctttgtt ccttctcttg tgtgaatttt aagatatgtt tgaagaatgt aatatccata
     2461 ataccagttc taccatgtct attcttttca acttttaatt ttaagttagc agtcttttga
     2521 tttctttctt catcttcttt attactataa ataaacataa caacgtcagc atcttgctcg
     2581 atactacctg attctcttaa ttgagctagg tttggctctc catcaggctt agctgctgtt
     2641 ctatttaatt gcgataaagc gataatggga atatcaagtt ccatagcaag tacttttaaa
     2701 gatcttgaaa tagcaccaac aatttccgct cttgtagaag aacgtcgagg attatctaaa
     2761 tctattagtt gtaaatagtc aactacaatc atatctaatt taccatttct ttttaaacta
     2821 cggcattgag ccataatatc aaaaatatta gcactattgc tatcatctaa atataaatta
     2881 aatttatcaa ttgtttgcga cttagctgtt agaatactag aaatctctgt acctgataat
     2941 tgacctttgt taattttatc atgatcaact gctgattcag tactaatcaa tctctttaca
     3001 atttgttctt tacccatttc aagggaaata aaagctacat attttccttg cttacatgct
     3061 gctgaagcaa agtttaaagc taatgcagtt ttaccaacgc ctggacgagc agctaaaatg
     3121 attaattctt gttttttgaa accagataaa atattattta attctttaaa tcctgtatca
     3181 ataccaatca aatcattttt gttttgcatt cttttgttta tctcatcaat aactccagga
     3241 gtaatagaat ctacttttgt aagttggtta actgatcttt tgtttagaac gttcttaact
     3301 aatctttctg tattatttaa aatatcatct gtttgaaaac gacctgataa aatatcattt
     3361 gaaagatttg atagtgtatg ataaagctct ctttgcaatg ctttttcttt aatgttagct
     3421 gcataagtat cccaacgaac atatgttggt agaatatcaa ttaaataacc aatatactca
     3481 attcccccag atgcttcaaa tttcttttct ctttttaact cctcgattac atttgtgtag
     3541 ttaatctctt ggtgattttg atatagtttt tccattgctt ccataatatt cttatgacgt
     3601 tgatcataga aatgttctga ataaacacta tttacaatag ctgaagcttc ttgaggatta
     3661 attaaagccg aagctaaaat atattgttct gattcgatac tataaggtaa ttgtcttagt
     3721 tcttccataa ttattccttt ccaacaacat aaattgttaa tctagcttta atatcaggat
     3781 gaagatcaac caaaactaca aatgtaccta aagcttgaat ttcatcttta ataatctttt
     3841 tacgatctaa agtaatatca tattgtgcct tatattcgtc tactatcatt ttactactta
     3901 ctgagccgaa aatttttcct tcttttccta ctttaacgcc aattgtaact gggtttgctt
     3961 caattttgtc acgtagatct ttcatttcat tttctttact taaagctaaa cgtttctctt
     4021 tttctttttc tttttctaat tctttgatat tatcaactga tgcaataatg gcttgtccac
     4081 ttcgaattaa gaagttacca tatccactct ttacatcgat aatatcgcct tttttacctt
     4141 ttcctttaac ttcagttgta agtataattc gcattgaatc tcctcctctc tcttctgaaa
     4201 tatcaatttc ttttataagc atatcttttg cttcttctaa atctacagct cttagttgag
     4261 ttgcggcatt gttataatga ccaccaccac ctaaagcttc cataattaat tgaacgttag
     4321 tttcgtctaa actacgagca ctaataccaa tttcattttc tgatattctg ccaacacaaa
     4381 acgaagcttt aatgcctttg atttctaaaa catcatcagc tactttagct aaaaactgtc
     4441 ttggattgac tccttctgta acagtagcaa ttccatattt ttctttataa attataagat
     4501 tagataaaac atcaattctc ttttcatatt tatcataatc ttcacgtaaa tactttttcg
     4561 ctagttgtaa ttctccacca taagtttgta atctagataa aacattaaat gttctataac
     4621 ttgcgcgata cattaaatca tttgtatcta ctaaaattcc cataatcatc catgtagctt
     4681 cagcactact tacaatagga tctttatcta aaaactcata catctctaca agcaactcta
     4741 cacttgaaga cgcagatgaa gtttgataaa tataatcgta tttatctacc aaaaacttac
     4801 caggacgatg atgatctata acagctacat gtttagcttt tttataaagt tttggttcca
     4861 ttaaaatctt ttgattttga acgtcaacga ttattaataa gtcactagct ttaatatctc
     4921 taattgcttt tttagatcct actaaataat ccaaagttgc agtatgttct ctttgaataa
     4981 tagaatataa atattgaatt gtttggtcaa ctttatctaa gtcaaaaaca attttcgcgt
     5041 ctctattata agcagcagca attttttgca ccgcaattgc cgctccaaaa gcatcagcat
     5101 cagcatcaat atgtgtcata atgtaaattt tttgagcttt attaattaat gaaactaact
     5161 cttcagcttt agttcttaca taaacaggag atctactttc aaatgaagca gtctttccac
     5221 caaagtaaga cgtctctcca tatttattta aaacgcattg gtttccgcca cgatttaaag
     5281 ctaggtttaa ttgagtttca gctaatgcaa ataactcaac atagcctaaa tcagcattat
     5341 cagcaacagc aattccaatt gatgcacttg atctaatatc ttcttttaaa caatatgact
     5401 taacatcatc aattacctta aattcatctt tcacaacttc tgctaattgc ttctcattta
     5461 ataaaattaa atattgattg tctgtatatc cttttaagta gatatcaaac ttactgcacc
     5521 aatcacttaa gataccaata atattactca tttgcgaaga tctctcttga gcatcaaagc
     5581 cagataaagc ttcacttaaa ttatctaagt tgataatacc agcagctagt gtatggtctg
     5641 catatttttg tgtcaaatta tggaattcag ttacgtcagt taaataaaca atattttctt
     5701 ccatcaaaac ttcacattca aaaattgaac cataaagtga tatttcaaac ttatcttcat
     5761 ttgataaatt gtaatataaa tcagcactaa tgttttcaat tctaacatca actaaaggac
     5821 ttaaaaagat ttccttagca taagcattgg cccactgaat tgttttattt ttatcataaa
     5881 caataatagc aataggtagt ttattaaagg cagtttctcc acctttttta acacggtaag
     5941 aaattgagtt ccataactca tacctttgtt ctaaccattg cattttccta aacttcttat
     6001 tcccaacatt taatacaaac acagttgtaa atgatgctgc tactatcacc aaagcagcat
     6061 aagttagcca ttcgtttatt tccccaaaaa tagctgtgaa aactaaaacg aaacaagaag
     6121 ctaagaaaag cactaatgat attattctat aaactaatgt attcctcata aaatcaccat
     6181 cttaactatt ttagtatacc atatttatag gaaaaaataa actctttatc ataaaaacaa
     6241 agaaaaaaga gtagcataaa ctactctttc acaaatggta acaatgccat atgacgtgct
     6301 ctttttattg caacagctaa ttgtctttga tagatagctt ttgtacctgt tgatcttctt
     6361 ggtttaattt taccacgatc agtaacgaat cttcttaata attcgtaatc agtccaatca
     6421 atcttttcaa ttttattatc tgtaaagtag caactttttc ttcttctcgg tctaaatgcc
     6481 atttacttaa cctccttaaa atggtatatc atcgtcagta atacctaagc cagcttgcat
     6541 ttccttaaaa gggtcttctt gcttgcgagg ttctggtttt tggttgaact caaaaggtga
     6601 atcttgacta aatgattgat atccttgcga actaccagca gattttgatt ctaaaaattc
     6661 aattgcattt gctagtattt ctgtaatata aaccatttta ttatcttggt cttgataact
     6721 tcttgttcta acctctccgt ctactccaat tagactacct ttacgcatat aacgagctag
     6781 gttctctgct tgtacgcgcc atgcaacaca gttgataaaa tcagcttcac gattgccttg
     6841 gcgatttaca taactgcggt taacagcgat tgtgaaaaaa caataactga ttccgctatt
     6901 tgttttttta agttctggat cttttgtaag tcttcctgtt aatacaactt tattcataat
     6961 taatccctca ctaaaatgtg tctaataata tcttctctga tattacaaat acggttatac
     7021 tcgctaaccg cttctggagt agcttctact ctgaacttta cataataacc ttttttaaga
     7081 tcattaattt cataagctaa atctttcata ccccattcat caatttccaa aacacgactt
     7141 ccatgttctt caaagatttt agcaatctcg ctaacaattg ctttgaattc ttcttcagtg
     7201 tgttcaggat gaatgatgta cataccagtg tattctctca ttcttccacc tccttctggt
     7261 cttttggctt ttatttctat aaaagcaagg acgaattttc attctttgtt tattatatac
     7321 tattaagtct tttttgtcaa ttatttcatt taaaaataac tagtttttac accaaaataa
     7381 agagttaact aaaatagtta actctaatta tatactatct ctttctcctg tttcttgctt
     7441 tggtataatt ctaattttct taagcaacca ataaataaag tccaaaacta aataaatacc
     7501 aaatccaaaa actaaaacta ataaataacc aactaaatct aacttcacat atcctaattc
     7561 taatggttca tttaagaaca atatcgataa tactgctcct gcagctgaaa caataaaaac
     7621 taaaaaccta taaaaattaa gtggtcttga tacaaaatat agtacataaa acataatgat
     7681 tgtagttaca tataaagtga ccgttgaata gccttcatta gttgatagaa ttgcaaaacc
     7741 atcaattttt gttaagaaat aaataatcgc atgagcaagt aaaacagtaa aagctccagg
     7801 caagacattt ctcaaaacat tactaataaa tcttccttta atcttttcgg tattaggctg
     7861 taaagcgaga agtgtagctg ccaaagctaa gacaaaccat tcaaacggaa ataactgatt
     7921 tggactaaat ggatatgccc ttctaactgc taaataaaat atcgttaata aaatagaaaa
     7981 taatgttttt gttaaaaaca atatagctgt acgttgaata ttgttagtaa ctcttctacc
     8041 ttcagctact atttctggca tagctgaaaa gttagattca agtaaaacaa tatgtgatac
     8101 gtgtttagta gcatcactac cgctagccat tgctatacta caatcagctt cttttaatgc
     8161 taagatatca ttaacaccat cacctgtcat tgcaacagtc tttttattca ttttaaatgt
     8221 ttgaactaat agtttctttt gataaggcga aacacgacca aaaacattat attctgttgc
     8281 tgctgctata acttcttcat cagataaacc atctaaacta atataacgag aggcatcgct
     8341 aacacctgct cttctagcaa tctcagcaac agtaattgga ttgtctccac taattacttt
     8401 tatatcaact tcattatttt taaaataagc tattacttgt tctgcatcat ctcttatatg
     8461 atcttgaata gcaataacag ctaatgctgt tgtagatttt ggtctagatg tgggattaat
     8521 tggacttttt gtgtgagcta aaacaatcac acgacagcct tgtgctgcaa atctttcaac
     8581 tcttctttga atctttgcat aattagtttc atttaaaaca atctctggtg cgccaattat
     8641 ataataccct agtttcccaa aacgaactgc actatatttt ctttgcgaag aaaacggaat
     8701 aatgccatct gcttttaaaa ttgagttctt accataatgt tcagccaaag cattactagt
     8761 agcattactt tcgggaaaag cattcatcat tgatcctata atttccctaa ttgtataatc
     8821 gccttttgtt tctaattgaa cactatcaat aactttcatt gttccatcag taatagtacc
     8881 tgttttatct aaacataaga tatctattct ggcaagcatt tcaatacaat atatttcttg
     8941 cactagaact ttcttctttg caagcctaat aacgctgaca gttaaagcta ctgttgttaa
     9001 taagaataac cctgcaggta tcattgaaat aaccgaacca gatgtactct taaatgcctc
     9061 agcaaatttt tcaaatcctt cccaagctct acctgtattt ggattaattt ctggaaggct
     9121 atcataacta tttaaatatg tagctacagc taatggaata ataataattg atactatttt
     9181 taaaatccag tttaacgaat ttaagatatc tgattttgca tatttgtgtt ttttagcacc
     9241 agttgccaat ttctctacat aacttgcaga accaacttta tcagctattg ctactcctac
     9301 cccaccaaca acataactac cagaatataa agtatctcct attttcttat gaatagctat
     9361 tgattccccg gtaatcaaag attcattaac ttctaagcta ccttctttta aaatacagtc
     9421 acaaccaatt tgtttacctg atttaaaagt taaaacatca tctaagacta cctcgtcaat
     9481 aggaatatct tcttgctcgc catttctaat aaccgaagca ctaggaacaa acattaaaga
     9541 taattgatca atttgttttt tagctcttgt ttcttgaata atcccaataa tcatattaga
     9601 taaaacagtt cctaaaaata acaaattagt atattcct
//
LOCUS       DAOSGS010000024         9612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6042_length_9612_cov_4.325834, whole genome shotgun sequence.
ACCESSION   DAOSGS010000024 DAOSGS010000000
VERSION     DAOSGS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_9612_cov_4.325834"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLQ22_01840"
     CDS             <1..225
                     /locus_tag="PLQ22_01840"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOH58735.1"
                     /translation="KSTTDTVLFARAKTEESYHHFKDLITSIKVYDETRIKEVTEECI
                     KNNISIGGSADILVVAIFLYLLREEFFGRE"
     gene            218..979
                     /gene="murI"
                     /locus_tag="PLQ22_01845"
     CDS             218..979
                     /gene="murI"
                     /locus_tag="PLQ22_01845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOH58736.1"
                     /translation="MNKKIGIFDSGLGGITVLNYLKDFYPNYDFIYLADSLNCPYGEK
                     TSKEIENLVTKNTLFLESLGVEVVVIACNTASANSGDVERYTQIPIVRIIKPTALAAL
                     KKTKTNNILVLATNLTIENKAYDKYLEGVNIYSVKGSRLVPIVESGLMDTKEADRVVL
                     ELLDGYQNKGIDTLILGCTHFPLLKMSIKKVMPDVVLVDSFEPVRGELEALIGKGEAG
                     KMQKITLYTTGKIEDFKKQLGIFHLENAIIKEARI"
     gene            complement(989..1537)
                     /locus_tag="PLQ22_01850"
     CDS             complement(989..1537)
                     /locus_tag="PLQ22_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOH58737.1"
                     /translation="MIKKLKTPLTDKDIESLRAGDIVLITGTIYTARDAAHQRLVKLI
                     KEGKDLPFDAKNAIIYYTGPAPKKPGRVIGPCGPTSSYRMDPYSPTLMKEGVRVMIGK
                     GDRSEEFYKALIDQKAVYLQATGGAATLISESVKNVTLIAYPELGAEAVYKFDVKEFK
                     AIVTNDSVGGNLPKENRKKYQK"
     gene            complement(1538..2380)
                     /locus_tag="PLQ22_01855"
     CDS             complement(1538..2380)
                     /locus_tag="PLQ22_01855"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOH58738.1"
                     /translation="MRKIDLKLIEDTVAELLYDACINLGKKELKLLEKAKLEEKSEIG
                     KDIISQILENDYLACKLDLPLCQDTGQAVIFLEIGSKVYFEGDLEEAINKGVAKAYTE
                     GYLRKSVVSNPLTRINTGDNTPAIIHTKIVPGDKIKITAAPKGGGAENCSALKMMIPA
                     DGIDGIKKFVLETVEKAGGKPCPPILVGVGIGGNLEKAAMLAKEAIILREVDDEAEGE
                     AERKLEKELLEAINNLGVGPMGLGGTKTALAIKVNTYPCHIASLPVAVNLQCHAMRHK
                     VKVI"
     gene            2462..3358
                     /locus_tag="PLQ22_01860"
     CDS             2462..3358
                     /locus_tag="PLQ22_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOH58739.1"
                     /translation="MKRELIKSSYPFGIIEYQKYLEYIHQKGPKKYKDMKSLIVFIYP
                     FSNTLVKGKYLPAKFAYGKDYHQEVKELIEDAIEDLKLTNYEIKVDVSSLKEKTCAYL
                     AGLGLIGKNSLLINEKYGSYIAIGIVITDEVFNEYDRPIEGDYCKDCNRCIKACPNKA
                     LSSDGLDKKKCLSYLNQSKSTDYLFFDKMKYLYGCDICQDVCPYNKIKDYGLESFLID
                     EEAYLSLEELKSMDEKEYLEKYQDKTFNWIGYLPMVRNLLVLKVNNKDITKKELEFFQ
                     EKHKSEKWFYDHIEYLKEKLDE"
     gene            3351..3866
                     /locus_tag="PLQ22_01865"
     CDS             3351..3866
                     /locus_tag="PLQ22_01865"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOH58740.1"
                     /translation="MNNIVLFEPEIPQNTGNIMRTALATNFKVHLIEPLGFKLDDVSI
                     KRSGANHVKYVDYFVYTSWDDFVSKNKGEFYFYTRYGLKTYTEVDLSDTSKNYYYIFG
                     KESTGIEKEILRNNLDRCLRLPMNEHMRSLNLSNTVAIICYDALRQQGFPGLSIFEPE
                     IQKGKDFLLKK"
     regulatory      3907..4082
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4162..5430
                     /locus_tag="PLQ22_01870"
     CDS             4162..5430
                     /locus_tag="PLQ22_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOH58741.1"
                     /translation="MKKRLMFVLFLLLSLLTFGCSCSQDKFSVEVLKLDGTALVEEKE
                     LKLVKDLDLVAVLKADEDIRLDGSDGEYGFYITKAGGYEANASENEFWQLSVNGKQSD
                     VGISALEVKAKDKITLQVTQWFDSLHVVIKDLEGTKLLDKTIEYLGYKESILDALLND
                     SEVGLEGSTGEYGFFITKVKGIEANSAQNEYWQILVDGVPSSVGISSLELAYGQTLSL
                     ELSQWQDLFSVVVKDLEGNELLNKSINYLAYKESVLDALLADSEVELAGSNSEYGYFI
                     TGVKGIEANGAQNEYWQILIDGEAASVGISSIELTYGQVLTFQLAQWYDSFSLIVKNL
                     AEEELFNDSINYLAYKESVLDALKNHEQVKLVTEIDGDKEAIISVCDCNLAANTVWYV
                     YKNGEVYRDDLNALTTSYGDTIEIKLLAIE"
     gene            5513..6391
                     /gene="thyA"
                     /locus_tag="PLQ22_01875"
     CDS             5513..6391
                     /gene="thyA"
                     /locus_tag="PLQ22_01875"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011283541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOH58742.1"
                     /translation="MKQYLDFLKHILEKGERRENRTAIETISTFGYQMRFNLEEGFPL
                     LTTKRTYLRAIIHELLWFISGNTNIKPLVDNNVRIWNEWPYEKYKKSKDYKGESLDEF
                     VAKIKDEEGFSEKYGDLGPVYGYEWRHFEGQDVFVDQLAKIIEEIKTNPHSRRLIVSA
                     WHPAYIERMALPPCHLLFQFYVQNGKLSLQLYQRSADAFLGVPFNIASYSLLVMMVAK
                     ICNLGVGEFVHTVGDAHIYVNHLEQVNLQLSRTPRSLPKMIIHGEQKTIDDFKFEDFE
                     LVDYNPHPTIKGEVAV"
     gene            6388..6858
                     /locus_tag="PLQ22_01880"
     CDS             6388..6858
                     /locus_tag="PLQ22_01880"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH58743.1"
                     /translation="MISLIVAMTRKGLIGKDNDLPWNYPEDLKYFKKTTLNKTVAMGE
                     KTFLSIYNRNQKPLPKRKNVVFTFDKDFKCEGVEVSHDLHKYLQEYKGDDLFIIGGRE
                     IYREALPYCKYLYITFIEKEYEGNVYFPKVNFDNYKIISEEKLGELTFTVFERR"
     gene            6861..7715
                     /locus_tag="PLQ22_01885"
     CDS             6861..7715
                     /locus_tag="PLQ22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH58744.1"
                     /translation="MFLFLTLLLATLLSFLYTYFLYDSESFTYWVFFLGIVISFFIVF
                     ILEVLIIWAFFIPVNKNKDVEKVKENRFYYFIVRQITYFLAQIFHVKMYVAGEEKLPK
                     DGFLAVGNHQSNFDPIASVWIFKKQRFKFVAKDVLFKFPLLGKALVGSGFLPLNRSDV
                     RQGIQVINASAERIKCGENIFIYPEGTRSKTRELIEFHAGSFKIAYKAKCPIAIIATD
                     GAGDLGKRWPLATKTFFKVCEVLEYDDYKDMNTQELKDYVYKVLDENIKDMRARIPSL
                     TKYLKKKD"
     gene            complement(7732..8295)
                     /locus_tag="PLQ22_01890"
     CDS             complement(7732..8295)
                     /locus_tag="PLQ22_01890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOH58745.1"
                     /translation="MKICFVRHGQTDANKNGLIQGVSDHPLNETGIKQAKEAAKLIKK
                     KFPYFNAIISSSLSRAKKTAEVIKEDLKFKGEIIIDDHFIERSFGPYENHKVTDEVYI
                     KLTSENTEGLEPFKDLQKRGIEGILELEKKYKGKNIIIATHAQILKAIVCFIDPKFDF
                     KTVIYNGSLNIISVKNQKMKIEKLNIY"
     gene            8411..9577
                     /locus_tag="PLQ22_01895"
     CDS             8411..9577
                     /locus_tag="PLQ22_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOH58746.1"
                     /translation="MSKLGTIALGIKAPIIKPEDDICKVVIESLKEAVKEDGLRIDDR
                     DILCITEAVVAKGQNNFASLDDIAFDVKRKMGKGKVGLIYPIFSRNRFAILLEGIAKG
                     VDELVLVLSYPKDEVGNSLVKDFHKYHLDLNKQSYTVKEFKEIIPEVKHEFTGVDYLE
                     LYESLGKGKITIVLSNNPLEVLKYTDKVIVGSIHNRNELKEMLLLNKAKKVITLAEIL
                     NEPVNGSGFNSEYGILGSNASTSHSVKLFPKRDQMLIERIQEAAFKEFGKNIEVMVYG
                     DGGFKDPVGGIWELADPVVSPMYSKGLEGTPNEIKLKYLADNDFKGKENIEDLVKERI
                     KAKEKELKGKAESLGTTPRRYTDLVGSLADLVSGSGDKGTPFVLVKNYFKNYSE"
BASE COUNT         3366 a         1166 c         1719 g         3361 t
ORIGIN      
        1 aaaagtacta ctgatactgt actttttgca agggctaaaa cagaagaaag ctatcatcat
       61 tttaaagatt taattacttc aatcaaagtt tatgatgaga caagaattaa agaagttaca
      121 gaagaatgta ttaaaaacaa tataagtatt ggtggatcag ctgatatttt agttgtagcg
      181 atatttttat atttattaag agaggagttt tttggccgtg aataagaaaa ttggtatatt
      241 tgattcagga ttaggcggaa ttacagtttt aaattattta aaagatttct atcctaatta
      301 tgattttatc tatttagctg atagtttaaa ctgtccttat ggagaaaaaa caagtaaaga
      361 aattgagaat ttagtcacaa aaaacacact ctttttagaa agcttagggg ttgaagtagt
      421 agttattgct tgcaatactg ctagtgcaaa tagtggtgat gtagaaagat atacgcaaat
      481 tccgattgtt agaatcataa aacctactgc tttagcagca ttaaaaaaga ctaaaacaaa
      541 caatatttta gtcttagcta cgaaccttac aattgaaaat aaggcttatg ataagtattt
      601 agaaggagta aatatttatt cagtaaaggg atcaaggctt gttcctatag tagaatcggg
      661 gttaatggat acgaaagaag cagatagagt agtcttagaa ttactagatg gttatcaaaa
      721 taaaggtatt gatacactaa ttttaggttg tacacacttt cctttattaa aaatgagcat
      781 caaaaaagtg atgccagatg ttgttttagt tgattctttt gagcctgtaa ggggcgaact
      841 tgaagctttg ataggtaaag gtgaagcggg aaagatgcaa aaaatcactt tatatacgac
      901 aggaaagata gaagatttta agaaacaact aggaattttt catttagaaa atgcaataat
      961 aaaagaagca aggatttaaa tccttgcttt atttttggta tttttttcta ttttcttttg
     1021 gtaagtttcc accaactgag tcgttagtta caattgcctt aaactctttt acatcaaatt
     1081 tatatacagc ttcagctcct aattcaggat aagctattaa agtaacattt tttactgatt
     1141 cactgattag tgtagcagca ccaccagttg cttgaagata gactgctttt tggtcaatta
     1201 gagctttata gaattcttct gatctatcgc ctttacctat cattactctt acaccttctt
     1261 tcataagagt aggtgaataa ggatccatac ggtaacttga tgtaggccca caaggtccaa
     1321 taactctacc tggtttttta ggtgctggac cagtatagta aataattgca ttcttggcat
     1381 caaaaggcaa gtctttacct tcttttatta actttactaa tctttgatga gcagcatcac
     1441 gagcagtata aatagttcct gttattaaaa caatatcgcc agcacgtaaa gattcaatat
     1501 ctttatcagt taaaggagtt tttaatttct taatcattta aatcaccttc actttgtgac
     1561 gcattgcgtg gcactgtaag tttactgcta caggtaggct cgcaatatga caaggataag
     1621 tatttacttt aattgctaga gcagttttag ttcctcctag tcccattggc cctacgccta
     1681 aattattgat tgcttctaag agttcttttt caagtttacg ttcagcttca ccttcagcct
     1741 catcatcaac ttcccttaag attattgctt ctttcgcaag cattgccgcc ttttctaaat
     1801 tgcccccaat cccaacccca actaaaattg gagggcaagg tttaccgcct gctttttcta
     1861 ctgtctctaa tacaaatttt tttataccat caattccatc agctggaatc atcattttta
     1921 aagcgctaca attttctgct ccgccacctt taggtgcagc agttattttt attttatcac
     1981 ccggaacaat ttttgtatgg ataattgcag gggtattatc tccagtattt attcttgtta
     2041 gggggttaga aacaactgat tttcgtaaat agccttcagt ataagcttta gcaactcctt
     2101 tgtttattgc ttcttctaaa tcaccttcaa aataaacttt tgagcctatt tctaaaaaga
     2161 ttactgcttg accagtatct tggcataaag gtaagtctag cttacaagct agataatcgt
     2221 tttctagaat ttggctaatt atatcttttc ctatttctga cttttcttct aattttgcct
     2281 tttctaaaag ctttaattct tttttaccta agttaatgca agcatcatat agtaattccg
     2341 ctactgtatc ttcaattagt tttaaatcta tttttctcat ttttatcacc ttgctttaat
     2401 tatactatat ttttagagat tttagtagtg atttgtgtta aaatatagag aaggtgagac
     2461 tatgaaaaga gagttaatta aaagtagtta tccttttgga attattgaat atcaaaagta
     2521 tttagaatat attcaccaaa aaggtcctaa aaaatataaa gatatgaaat cattaattgt
     2581 tttcatttat cctttttcca acactttagt taaaggtaaa tatttaccag ctaagtttgc
     2641 ttatgggaaa gactatcatc aagaagttaa ggagttaatt gaagatgcaa ttgaagatct
     2701 taaactaact aactatgaaa ttaaagttga tgtaagtagc ctaaaagaga aaacttgtgc
     2761 ttacttagca ggtttaggtt taattgggaa gaatagttta cttattaatg aaaagtatgg
     2821 atcttatatt gcaattggaa ttgtaattac tgatgaagta tttaatgagt atgatagacc
     2881 aattgaaggt gattattgca aggattgtaa taggtgtatt aaggcttgcc ctaacaaggc
     2941 attaagcagt gatggcttag ataagaagaa atgcttatct tatttgaatc aaagtaaaag
     3001 tactgattat ttattttttg ataagatgaa gtatttatat ggttgcgata tatgccaaga
     3061 tgtgtgtcct tataacaaaa taaaagatta tggtttagaa agttttttaa ttgatgaaga
     3121 agcatattta tcattggaag agttaaaatc aatggatgaa aaagaatatt tagaaaagta
     3181 tcaagataaa acttttaact ggattgggta tttaccaatg gttcgtaatt tgttggtatt
     3241 aaaggtaaat aataaagata taactaagaa agaattagaa ttctttcaag aaaaacataa
     3301 gagtgaaaaa tggttttatg accatattga atatttaaag gagaagttag atgaataata
     3361 ttgttttgtt tgaacctgag attcctcaaa atacaggaaa tataatgaga acagccttag
     3421 ctactaattt taaagttcat ttaattgaac ctttaggatt taagttagat gatgtttcaa
     3481 taaaaagaag tggagctaat catgtaaagt atgtagacta ttttgtttat acatcttggg
     3541 atgattttgt atctaaaaat aaaggcgaat tctactttta tacaagatat ggtttaaaaa
     3601 cttataccga agttgattta agtgatacta gtaaaaacta ttattatatt tttggaaaag
     3661 aatctactgg tattgaaaaa gagattttaa gaaacaattt agataggtgt ttgagactac
     3721 cgatgaatga acatatgagg tcactaaatt taagtaatac agtagcaatt atttgctatg
     3781 atgcattaag acaacaagga tttccaggac tttcaatctt tgaaccagaa atccaaaaag
     3841 gtaaggactt tttactaaag aagtaaaaag tctttttact tttaattgac aaaaacttta
     3901 aaggtgatta taatatagat acttggtgta tacacataaa aaggaattag gttatgccta
     3961 agctgtccca gcagccgttg gagatgatga aagatttaag aaccactgat tttaatcggg
     4021 aaggtaaatt gagtaaagtg aatctctaag ccggaagact taccaagtat taaaaaacta
     4081 acattccctg ggggtggaaa tggggctttt ttcatttggt cctattttta ccaaaaaatt
     4141 taaaaatagg aggtttataa aatgaaaaaa agattaatgt ttgttttgtt tttactactt
     4201 tcattactta catttggatg ttcatgtagt caagacaaat tcagtgttga agttttaaaa
     4261 cttgatggaa cggctttagt tgaagaaaaa gaactaaagt tagtaaaaga tctagatctt
     4321 gtagcggtat taaaagctga tgaagatatt agattagatg gaagtgatgg agaatatggt
     4381 ttctacatta ctaaagctgg tggttatgaa gctaatgctt cagaaaatga attctggcag
     4441 ctttcagtaa atggaaaaca atctgatgtt ggtattagcg ctttagaagt aaaagctaaa
     4501 gataagatta ccttacaagt aactcagtgg tttgatagtc ttcatgtagt tattaaagac
     4561 ttagaaggaa ctaaattact tgataaaact attgaatatt taggatataa ggaaagtatt
     4621 ttagatgcat tattaaatga tagtgaagtt ggtttagaag gtagtacagg tgaatatggc
     4681 ttctttatta ctaaggtaaa aggaatagag gctaatagtg ctcaaaatga gtactggcaa
     4741 attttagtag atggtgttcc ttcatcagtt ggtatttctt cattagaact tgcttatggt
     4801 cagactttaa gtttagaact atcacaatgg caagatttat ttagtgttgt agttaaagat
     4861 ttggaaggaa atgaactttt aaataaatct attaattact tagcgtataa agaaagtgta
     4921 ctagatgcct tgcttgcaga tagtgaagta gaactagcag gttctaatag tgaatatgga
     4981 tattttatta ctggagttaa aggtattgaa gctaatgggg ctcaaaatga atactggcaa
     5041 attctaatag atggagaggc agcatcagtt ggtatttctt caattgaatt aacttatggt
     5101 caagtactaa catttcaatt agctcaatgg tatgatagtt ttagtttgat tgtaaagaat
     5161 ttagcagaag aagagttgtt taatgatagt attaattatt tagcttataa ggaaagtgtc
     5221 ttagatgctt taaaaaatca tgaacaagta aaactagtta cggaaattga tggtgataaa
     5281 gaagcaatta taagtgtttg tgattgtaat ttagctgcta atacagtttg gtatgtatac
     5341 aaaaacggag aagtttatag agatgattta aatgctctaa caactagtta tggagataca
     5401 atcgaaatta aattattagc aattgaataa taaaaataaa aagaagcatt tgcttctttt
     5461 ttgttttgaa aatgggtttg tttatgatat acttatacta aggtgattaa ttatgaaaca
     5521 atatttagat tttttaaaac acattttaga aaaaggtgaa agaagagaaa acagaacagc
     5581 gattgaaaca atctctacat ttggttatca aatgagattt aatttagaag aaggctttcc
     5641 tcttctaaca actaaaagaa cttacttaag agcaattatt catgagcttt tatggtttat
     5701 tagtggaaat acaaatatta agccattagt agataacaat gttaggattt ggaatgaatg
     5761 gccatatgaa aaatacaaga agtctaaaga ctataaaggt gaatctttag atgagtttgt
     5821 agctaagatt aaagatgaag aaggtttttc ggaaaagtat ggagatttag ggcctgttta
     5881 tggatacgag tggcgtcatt ttgaaggcca agatgtgttt gtagatcagc ttgctaagat
     5941 tattgaagag attaaaacta atcctcattc aagaagatta attgtctctg cttggcatcc
     6001 agcttatatt gaaagaatgg ctttaccgcc atgtcatttg ctattccagt tttatgtgca
     6061 aaatgggaaa ttatcgttac aattatacca aagatcagct gatgctttct taggtgtacc
     6121 ttttaatatt gcatcatatt cattacttgt aatgatggta gcaaagattt gtaacttagg
     6181 tgttggcgag tttgttcata cagttggtga tgctcatatt tatgttaacc atttagagca
     6241 agttaattta cagttatcaa gaactcctag aagcttaccg aagatgatta ttcatggcga
     6301 gcaaaagaca attgatgatt ttaagtttga agattttgag cttgtagact ataatccgca
     6361 tccaacaatc aaaggagaag ttgctgtatg atcagtttga ttgttgcgat gacaagaaaa
     6421 ggattaattg gaaaagataa tgatttacca tggaactatc ctgaagattt aaagtacttt
     6481 aagaaaacta ctttaaacaa aacagttgct atgggagaga aaacttttct atcaatctat
     6541 aatcgcaatc aaaagccttt accaaaaaga aagaatgttg tttttacttt tgataaagat
     6601 tttaaatgtg aaggtgttga agtttctcat gatcttcata agtatttaca agagtataaa
     6661 ggtgatgatt tatttattat cgggggaaga gagatttata gagaagctct tccttattgt
     6721 aagtatttat atattacttt tattgaaaaa gaatatgaag gtaatgttta tttcccaaag
     6781 gtaaactttg ataattacaa aattataagt gaagaaaagt taggtgaact tacatttacc
     6841 gtctttgaga ggaggtaggt atgtttctat ttcttacttt gcttttagca actcttttaa
     6901 gtttcttata tacttacttt ttatatgata gtgaaagttt tacttattgg gttttctttt
     6961 taggtatagt aatttctttc ttcattgtct ttattttaga ggtattaata atttgggctt
     7021 tctttattcc tgtgaataaa aataaagatg tcgagaaagt taaagaaaat aggttttatt
     7081 attttattgt aagacaaatt acttattttt tagctcaaat ctttcatgta aagatgtatg
     7141 tagctggaga agagaaatta ccaaaagatg gttttttagc tgtaggtaat caccaatcta
     7201 attttgatcc gattgcttca gtttggatct ttaaaaaaca aagatttaag ttcgtagcta
     7261 aagatgtatt gtttaagttt cctttacttg gaaaagcttt agtgggttca ggatttcttc
     7321 ccttaaatag aagtgatgtt agacaaggaa ttcaagtaat caatgcatca gcagagcgta
     7381 ttaaatgtgg agaaaatatc tttatttatc cagaaggaac aagaagtaaa acaagagaat
     7441 taattgaatt tcatgcaggg tcctttaaga ttgcctataa agctaaatgc ccgattgcga
     7501 ttattgcaac tgatggagca ggtgatttag gtaaaagatg gcctcttgct actaaaacat
     7561 tttttaaggt ttgcgaagtt ttagagtatg atgactataa agatatgaat acgcaagagt
     7621 taaaagacta tgtttataaa gtattagatg aaaatattaa agatatgagg gcaaggattc
     7681 cttcccttac taaatattta aagaaaaaag actaatttaa ttagtctttt tttaatagat
     7741 atttaatttc tcaattttca ttttttgatt cttgacagaa attatattta aactaccgtt
     7801 ataaataaca gttttaaaat caaacttagg atcaataaaa caaacaattg cttttaggat
     7861 ttgagcatga gttgcgataa taatgttttt acctttgtat tttttttcta attctaagat
     7921 tccttcaatc ccgcgttttt gtaagtcttt aaaaggttct aacccttctg tattttcact
     7981 agttagtttg atgtatactt catcggtaac tttatgattt tcataagggc caaagcttct
     8041 ttcaataaaa tgatcatcaa taatgatttc acctttaaat tttaaatctt cttttataac
     8101 ttcagctgtt ttcttagcac gagataatga tgaagaaata attgcattaa aataaggaaa
     8161 tttctttttt attagtttag cggcttcctt tgcttgtttt attcctgttt catttaaagg
     8221 atggtctgaa actccttgaa ttaaaccatt tttattggca tctgtttggc catgtcttac
     8281 aaaacaaatc ttcatattat acctccttat actagtttta agtatatcat atttatcctt
     8341 attttcaaaa catttcatat tgaaactttc gtaaattttt tgtataataa aatatataag
     8401 gagtattaat atgagcaagt taggaacaat cgcattagga ataaaagctc caatcattaa
     8461 accagaagat gatatttgca aggtagtaat tgaatctttg aaagaagcag taaaagaaga
     8521 tggtttaagg attgatgatc gtgatatttt atgtattaca gaagctgtag tagctaaggg
     8581 gcaaaataac tttgctagtt tagatgatat tgcctttgat gtaaagcgta aaatgggaaa
     8641 aggaaaggta ggcttaatct atccgatttt ttcacgtaat cgttttgcga ttcttttgga
     8701 aggaattgct aagggtgttg atgaactagt tttagtttta agttatccta aagatgaagt
     8761 tgggaattct ttagtaaaag atttccataa atatcatctt gacctaaata aacaaagtta
     8821 tacagtaaaa gaatttaaag aaattattcc ggaagttaaa cacgagttta caggagttga
     8881 ctatttagag ttatatgaaa gtttaggaaa aggtaaaatt acaattgttt taagtaataa
     8941 tcctttagaa gtattaaagt atacagataa ggtgattgtt gggagtattc ataatcgaaa
     9001 tgaactaaaa gaaatgttac tacttaataa agctaaaaaa gtcataacat tagcagaaat
     9061 cttaaatgaa cctgttaatg gctcaggttt taatagtgag tatggtatat taggctctaa
     9121 tgcttccaca agccattcag ttaagctttt tcctaaaaga gaccaaatgc ttattgaaag
     9181 aattcaagaa gctgcattta aagagtttgg aaaaaatatt gaagtaatgg tttatggcga
     9241 tggtggtttt aaagatcctg ttggtggaat ttgggaactt gcagatcctg tagtttctcc
     9301 aatgtattca aaaggactag aaggaacacc aaatgaaatt aagcttaagt atttagctga
     9361 taatgatttt aaaggtaaag aaaatattga agatttagta aaggaaagaa tcaaagcaaa
     9421 agaaaaagaa cttaaaggta aagcagaatc tttaggaaca acaccaagaa gatatactga
     9481 tctagttggt agtttagctg atttagtaag tggaagtgga gataaaggta caccttttgt
     9541 tttggtgaaa aattatttta agaattattc agaataataa ggacttgttt ttttaagtcc
     9601 tttttaaaaa gt
//
LOCUS       DAOSGS010000025         9278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6333_length_9278_cov_4.283964, whole genome shotgun sequence.
ACCESSION   DAOSGS010000025 DAOSGS010000000
VERSION     DAOSGS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_9278_cov_4.283964"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLQ22_01900"
     CDS             <1..479
                     /locus_tag="PLQ22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOH58747.1"
                     /translation="ERIMVSDKILGFHDLYIHSYGSGRKFVSVHVEMDSNLSLLECHH
                     IVDEIERDFKSNYDIDITIHIDPIEAETEEEREIKKELTKILKSYNEEFTFHDFRTVK
                     RKGLLDVIFDVAIPDSYKVKDSKFLEKLKKDMYRYNKNLRLFVSIDRHYIREEENE"
     gene            472..1752
                     /locus_tag="PLQ22_01905"
     CDS             472..1752
                     /locus_tag="PLQ22_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOH58748.1"
                     /translation="MNKEVYKQIREDYFKKVENDSLTILFSGKAPQRSGDQSHSFSVN
                     RNFYYLTGINQDNVIVALLKSEKENKEYLFIQETTELMAKWDGRKLSIEEAKEISGID
                     NVVYLDKFDNYLFGFFNSMRFSESQVKTVLLDLERRNDPNYHSLALDFLADFSKKYPE
                     IKIANTYNNIISLRTVKKEAEIKKIQEAIDVTKGGIEALMINARSGIYEYQLESFFDQ
                     YLRYNGQRRNSFKTIAGSGVNATILHYSKNNTIIGEGELILFDLGAEVDYYIADITRT
                     FPISGKFSKRQKEVYQAVLNVNKKCIEYLKPGITWKELNLYANKLIIEELKGLCLIKE
                     DHELVKYYWHSIGHSIGLDTHDPSLYTVPLQEGMVITIEPGIYIEDEKIGVRIEDNVL
                     ITKEGPVNLSKDIVKEVKDIEAFMKKNNKYQKAK"
     gene            1819..2460
                     /locus_tag="PLQ22_01910"
     CDS             1819..2460
                     /locus_tag="PLQ22_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58749.1"
                     /translation="MKRILIFILVFMLCIPVNVKAYEPTGHKGILDVEFENGGKLLAR
                     MRQSDYQIVNSIVNKKKFFGWRTAYIHINEKAQYIGETVFSKANQTDKPFTMNYVLKE
                     DYKTKTTYTISGKISGQVKASMKIKLVNLEGTQSGEISGSYTGSTENSKSEETKMTIT
                     IPPYKKLTYRVTGECYVTSGVCQYYAVWIKIYRGQFEVIDVTTRYYELLEEDL"
     gene            2457..3407
                     /locus_tag="PLQ22_01915"
     CDS             2457..3407
                     /locus_tag="PLQ22_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58750.1"
                     /translation="MKKLWIIIGTISITLMVIVLLIVFVPKSQALDYTSFPKNYNYLE
                     NDGDIEIEFPIFYSEKENILIQKENVNDSLLTSKDESIIVPLNIESITYQEAIVLKEK
                     TFYKYLYKFSFKVKELNDYTILIPDAYLKIIYKDTKALKLHLGSFSYYQEESFNTNNN
                     DLRITYLKGIVNEIDGRKRVVAIDLKIANNTNKTLVLNSMNIYDQNIKVSHSLIQEVD
                     YDLASNELISNVVDNYNYREIDTSSYSLELEPYEEKHLIIPLGYKDDVVANEFAFRID
                     YLKDEEENSYYLGKFLFFEETRYTENELAKMIVYRYDHSS"
     gene            3391..4005
                     /locus_tag="PLQ22_01920"
     CDS             3391..4005
                     /locus_tag="PLQ22_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58751.1"
                     /translation="MTIVLKDIYKKYKKLVVLDFNFLEINEGYSYMLLGENGSGKSTL
                     IKMILNHTKYEGKITNTFRTISYIPERVNLPLFMKVREFLENIIMIRGVKTFDLDYLL
                     EYFELKVKEKQVIKNLSKGMRQKLILITGLALDVDLYILDEALNGLDKKMQDKLLKYL
                     EELKTKGKTIIYTTHYEKYFTNFSDYIIRLEDHKVYVSKNQTSS"
     gene            4061..>4379
                     /locus_tag="PLQ22_01925"
     CDS             4061..>4379
                     /locus_tag="PLQ22_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58752.1"
                     /translation="MIAILAIVSDLDPNIYSINQYYQSSYLLFSIFSNSFACFLFSFN
                     YLKEQDEYRYLIVLNNKDKISYLTSKLLVSILILILFHLIVVLIINVLGFIFLKVTIN
                     SYYL"
     assembly_gap    4380..4479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4480..4806
                     /locus_tag="PLQ22_01930"
     CDS             <4480..4806
                     /locus_tag="PLQ22_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58753.1"
                     /translation="INVLGFIFLKVTINSYYLKISVNTLFLNLLFGFMSLILIKVSNR
                     ILIIFVPLFVSFMINFIENKSFKKIVSVLFPVLDNNFLFNCWHFLFLYFVCLVIIYFL
                     EVYLDY"
     misc_feature    4869..4989
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4998..5531
                     /gene="infC"
                     /locus_tag="PLQ22_01935"
     CDS             4998..5531
                     /gene="infC"
                     /locus_tag="PLQ22_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOH58754.1"
                     /translation="MLFIKNLNSKNENLVNEEIPFPKVLTIDANGDALGVLPTKEAIQ
                     KAYEAGYDLVCVAPQAKVPVCRFMDYAKYRYEQKKRAKDARKNQKTITVKEIRLSPVM
                     DTNDLNVRIKQGKEFLAAGYKVKLNLSYRSRRMLATQGFDLTMINRYIEAVKEEAIVE
                     SEPKVDGRSAIAILRAK"
     gene            5551..5760
                     /gene="rpmI"
                     /locus_tag="PLQ22_01940"
     CDS             5551..5760
                     /gene="rpmI"
                     /locus_tag="PLQ22_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOH58755.1"
                     /translation="MPKIRTHKGLKKRIKISATGKVMRHRPGTSHLAPGKTQKRIRRL
                     RKGALVHTSDLNRIRKHVYVIRKGK"
     gene            5762..6121
                     /gene="rplT"
                     /locus_tag="PLQ22_01945"
     CDS             5762..6121
                     /gene="rplT"
                     /locus_tag="PLQ22_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOH58756.1"
                     /translation="MARVRGSVATRSRRKKVLKLAKGYFGSKHTLYRTANQQVMKSLT
                     YAYRDRKARKREFRKLWIQRINAAARLNGLSYSTLMHGLKLAKVDINRKMLSEIAIHN
                     PEDFSKLCKSAEKALSK"
     gene            6225..7895
                     /locus_tag="PLQ22_01950"
     CDS             6225..7895
                     /locus_tag="PLQ22_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH58757.1"
                     /translation="MREFTLKVNGKEYIKKDKVYLRDLAKELKIEAYLAKVDTRLREL
                     DYYVNYDAEVEFLNLTHVESMNAYETTLRFLTIIALENLYPGVEVKFRLGVSRSTYGE
                     IKDIMVDDALLEKLEKEMKKLIEKDQDIVRVKLSKEEAKEIYLKQGYHDKIETLKYRP
                     EDTVNLYKCNGYYNYLFNYMLPSMGYIKDFKLIPYYPGFIILYPRSELGGKMPEFHDS
                     VSFAKMLRAASRWSKQLNCETIANLNTHASNDSIIDLVNVCETKHNNMLAELGDKIKY
                     DIENIRLIAIAGPSSSGKTTFSNRLRGVLMAKGIEPLKISMDDYYKPRGKAPLDENGE
                     LDLEHIEALDIDRFNRDLLNLIQGKKVTLPHFDFNKGYSVDGKTIKISKSTPIIIEGI
                     HALNEKLTSLIPRHQKFKIYISPLFEISYDNHTPISATEIRQLRRVVRDAKYRGTNVR
                     DTFKMWPSVRKGEFRWIYPYQEEADYIFNTELTYELGVMKKYALPVLNEVPREDEYFI
                     DANRLVKFLKLVKDIPDEYVPCNSLLREFIGGSIFYSYERKEQEDLLS"
     gene            7861..8328
                     /locus_tag="PLQ22_01955"
     CDS             7861..8328
                     /locus_tag="PLQ22_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOH58758.1"
                     /translation="MKEKNKKTYFLKQNNGLLTAGSIDNFNTMTIGWSHYGYVFGLET
                     IIVYVRKSRYTFDFISKNDYFTVSFYSDEHKDDLEYLGTKSGRDEDKVSKTSLTPVKY
                     FDSVSFKEAKATFYLKKIYQGEIFEDQLSSPVKEKYFVEGNNHYLFIGQVIEK"
     gene            8325..>9278
                     /locus_tag="PLQ22_01960"
     CDS             8325..>9278
                     /locus_tag="PLQ22_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOH58759.1"
                     /translation="MKYILAIDQGTTSSRAILFDKKANIVASSQLEISSITNKSGWVE
                     QDPLELVESVRQVIIGVLNKANISAKDIEAVGITNQRETTILWDKKTGKPVNKAIVWQ
                     SRQSEKICKEWIEKGYNDKVLEKTGLIINPYFSASKIKWILENTKGLEDKLSKGEILF
                     GTVDTYLIWNLSKGKLHITDYTNASRTMLFNIKELKWDDELLEKFALPKSILPKVVPS
                     SGNLGKIEILGEEVNLRAIAGDQSAALFGQCCFDLGDVKNTYGTGCFALMNTGSEVIT
                     SKNGLLSTIAWHIDGKTTYALEGSVFVAGAAVQWLRDSMKFF"
BASE COUNT         3502 a          934 c         1601 g         3141 t
ORIGIN      
        1 aagaaagaat aatggttagt gataaaatct taggatttca tgatttatat attcattcat
       61 atggtagtgg aagaaaattc gttagtgttc atgtagagat ggatagcaat ttaagtttat
      121 tagagtgtca tcatatagtt gatgagatag agcgagactt taagagtaac tatgatattg
      181 atattactat tcatattgac ccaattgaag cagagactga agaagaaaga gaaataaaga
      241 aggaactaac gaaaatctta aagagttata atgaagaatt tacttttcat gattttagga
      301 cagtaaagcg taaagggcta ctagatgtta tttttgatgt agctattcct gatagttata
      361 aagttaaaga tagtaagttt ttagaaaaac taaagaaaga tatgtatagg tataacaaaa
      421 atttaagatt gtttgtaagt atagatcgtc attatataag ggaggaagaa aatgaataaa
      481 gaagtttata aacaaataag agaggattat tttaaaaaag tagaaaacga tagtttaact
      541 atcctatttt caggtaaagc acctcaaagg tcaggagacc aaagtcatag cttttctgta
      601 aatcgtaatt tctattattt aactgggatt aatcaagata atgttattgt agcattatta
      661 aaatcagaaa aagaaaacaa agaatattta tttattcaag agactactga gttaatggct
      721 aaatgggatg gaagaaaact ttcaattgag gaagctaaag aaatctcagg aattgataat
      781 gtagtctatt tagacaagtt cgataattat ttatttggat tttttaacag tatgagattt
      841 tctgaaagtc aagtaaaaac agttttactt gatttagaaa gaagaaatga tcctaattat
      901 catagtttag ctttagattt tttagctgat tttagtaaaa aatatccaga aattaaaata
      961 gctaatactt ataataatat tatctcttta agaacagtta agaaagaagc agaaattaag
     1021 aaaattcaag aagcaataga tgttactaaa ggcgggattg aagctttaat gattaatgca
     1081 agaagcggta tttatgagta tcaattagag tctttctttg atcaatattt acgttataat
     1141 ggtcaaagaa gaaattcatt taaaacaatt gctggttctg gagtgaatgc aactattctg
     1201 cactattcta agaataatac gattattggg gaaggtgaat taattttatt tgatttaggg
     1261 gctgaggtag attattatat cgcagacatt acaagaacat tcccaatttc aggtaagttc
     1321 tctaaaagac aaaaagaggt ttaccaagct gtattaaatg taaataagaa atgtattgaa
     1381 tatttaaaac ctggtattac ttggaaagaa ttaaatttat atgcaaataa attaatcatt
     1441 gaagagttaa aaggtttatg tttgattaaa gaagatcatg aacttgtaaa gtactactgg
     1501 cattcaattg gtcactcaat tggacttgat actcatgatc ctagtttata tactgtgcca
     1561 ttacaagaag gaatggtaat tacaattgaa ccaggaattt atattgagga tgaaaaaatt
     1621 ggtgttagaa ttgaagataa tgttttgatt actaaagaag gaccagtaaa cttaagtaaa
     1681 gacattgtta aagaagttaa agatattgaa gcatttatga aaaagaataa caaatatcaa
     1741 aaggctaagt aattagcctt tttttataaa gtaatttatt acctttttag ataatatatt
     1801 attgaaagag gtgataaaat gaaaagaata ttgatattta ttttagtatt tatgctttgt
     1861 attcctgtga atgtaaaggc atatgagcct acaggtcata aagggatttt agatgtggag
     1921 tttgagaatg gtggtaagct tctagcaaga atgagacaat cagattatca aatagtaaat
     1981 agtattgtta ataagaaaaa gttttttggt tggcgaacgg catacattca tattaatgag
     2041 aaagcacagt atattggtga aacagttttt tctaaagcta atcaaacaga taagccattt
     2101 acgatgaact atgttttaaa agaagattat aaaacaaaaa ctacatatac tatttctggt
     2161 aaaattagtg gacaagtaaa agcatcaatg aaaattaagt tagttaattt agagggaact
     2221 caaagtggag aaatttctgg tagttataca ggtagtacag aaaactccaa aagtgaagaa
     2281 acaaaaatga caattactat tcctccatat aagaaactta cctaccgtgt tactggtgaa
     2341 tgttatgtga ctagtggagt ttgtcagtat tatgcagttt ggattaagat ttatcgtgga
     2401 cagtttgagg ttattgatgt aactacaaga tactatgaac ttttagagga agatctatga
     2461 aaaagttgtg gattattatt ggaacaatta gtattacctt aatggtaatt gttttattaa
     2521 ttgttttcgt tcctaaatct caagctttag attatacctc ttttcctaaa aactataact
     2581 atttagaaaa tgatggtgat attgaaattg agtttcctat cttttattca gaaaaagaaa
     2641 atattcttat acaaaaagaa aatgtaaatg actctttact tacaagtaaa gatgaaagca
     2701 taatagttcc attaaatatt gaaagtatta cttatcaaga agcaatagtt ttaaaagaaa
     2761 agacttttta taagtattta tataagttta gttttaaagt aaaggaatta aatgattata
     2821 cgattttaat tcctgatgct tatttaaaga ttatctataa agatactaaa gccttaaaac
     2881 ttcatttagg tagttttagt tattaccaag aggaaagttt taatacaaac aataatgatt
     2941 taagaattac ttacttaaaa ggaatagtta atgaaattga tggaagaaaa agagttgtag
     3001 caatagattt aaaaatagct aataatacaa acaaaacatt agttttaaat agtatgaata
     3061 tctatgatca aaatattaaa gttagtcatt ctttaatcca agaagtagat tatgatttag
     3121 caagtaatga gttaattagt aatgttgttg ataattataa ctatcgtgaa attgatacaa
     3181 gtagttatag tttagagtta gaaccttatg aagagaaaca tttaataata cctttaggat
     3241 ataaagatga tgttgtagct aatgaatttg cttttagaat tgattactta aaagatgaag
     3301 aagaaaatag ttattattta ggaaagtttt tgttttttga ggaaactaga tatacggaaa
     3361 atgaactagc aaagatgatt gtttatcgtt atgaccatag ttcttaaaga tatttataaa
     3421 aagtataaaa agttagtagt tttagatttt aactttctag agataaatga aggttatagt
     3481 tatatgttgc ttggggaaaa tggttcgggt aaatcaacat taatcaaaat gattttgaat
     3541 catacaaaat atgaaggtaa gattacaaat acttttagga ctatttctta tattccagaa
     3601 agagtaaact tacctttatt tatgaaagta agagagtttt tagaaaatat tattatgata
     3661 agaggagtta aaacttttga tttagattat cttttagaat actttgaatt aaaagtaaaa
     3721 gaaaaacaag taataaaaaa cttatctaaa ggaatgagac aaaagttaat tttaataact
     3781 ggtcttgcct tagatgtaga cttatatatt ttagatgaag cattaaatgg tttagataaa
     3841 aagatgcaag ataaattatt gaaatattta gaagagctaa aaacaaaggg caaaacgatt
     3901 atctatacta ctcactatga gaagtatttt actaattttt cagactatat aataagacta
     3961 gaggatcata aagtctatgt cagcaaaaat caaacttcat cttaattatt tttttaataa
     4021 agtcaattta tttatttttt tatcttcctt agttttaatc ttgatagcaa tactagcaat
     4081 agtttctgat ttggatccaa atatttatag tataaatcag tactatcaaa gtagttattt
     4141 gctttttagt atttttagta attcttttgc ttgttttttg ttttctttta attatttaaa
     4201 agagcaagat gaatatcgct atttaattgt cttaaataat aaagataaaa tttcttattt
     4261 aacatctaaa ctactagtaa gtatattgat attaattttg tttcatttaa tagtagtttt
     4321 aataattaat gttttaggat ttatcttttt aaaggttaca attaattctt attatttaan
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttaatgtttt aggatttatc
     4501 tttttaaagg ttacaattaa ttcttattat ttaaagataa gtgttaatac tttgtttttg
     4561 aatttacttt ttggttttat gagtttaatt ttgattaagg taagtaatag aatcttgatt
     4621 atttttgttc ctttgtttgt ttcttttatg attaacttta ttgaaaataa aagttttaaa
     4681 aagatagtat cagttttatt tccagtattg gataataact ttctttttaa ctgttggcat
     4741 tttctttttt tgtattttgt ttgtttagtc attatctatt ttttagaggt ttatttagat
     4801 tattaactag tattttaaag attttactct tgacaaaaac aacttaataa tgtataatac
     4861 ttaagcatag tggaaagaag aagtcccact tctcacctga tcgatgttag ttggtaggtt
     4921 gatgtcactt aagatattat tatcttttga tttttagtgg gcagattatg ctcacttttt
     4981 tttgctatga gatggaggtg ctatttataa agaatttaaa ttcaaagaat gaaaatttag
     5041 ttaatgaaga gatccctttt ccaaaagtct taacaattga tgctaatggt gatgcattag
     5101 gagtattacc aacaaaagaa gcaattcaaa aggcttatga agcgggatat gatttagtat
     5161 gcgttgctcc ccaagcgaaa gttccagtgt gccgttttat ggattatgcg aagtatcgct
     5221 atgagcaaaa gaaaagagca aaagatgctc gtaaaaacca aaaaactatt actgtgaaag
     5281 aaattagatt atctccagta atggatacta atgatcttaa tgttaggatt aaacaaggaa
     5341 aagaattcct agcagcagga tacaaagtaa aacttaattt aagttatcgt agtagaagaa
     5401 tgctagctac tcaaggtttt gatctaacaa tgattaatcg ttatatcgaa gcagtgaaag
     5461 aagaagcgat tgtagaaagt gaacctaaag ttgatggtag aagtgctatc gcaattttaa
     5521 gggcgaaata gatagaggag gaatatagag atgccaaaaa ttagaacaca taaaggctta
     5581 aagaaaagaa ttaaaatttc tgcaactggc aaagtaatga gacacagacc aggaactagt
     5641 cacttagctc caggtaaaac acaaaaaaga attagacgtt taagaaaggg tgcactagta
     5701 catacatctg atttaaatag aattagaaaa catgtatatg tgattagaaa gggtaaataa
     5761 gatggcaaga gttagaggaa gcgttgcaac acgcagtaga agaaaaaagg ttttaaaatt
     5821 agctaaagga tattttggtt caaaacatac tttatatcgt acagctaacc aacaagtgat
     5881 gaagtcactt acatatgctt atcgtgatcg taaagcacgt aaaagagaat ttagaaaact
     5941 ttggattcaa agaatcaacg cagctgctag attaaatggt ttaagctata gtacattaat
     6001 gcatggttta aaattagcta aggttgatat caatcgtaag atgttatcag aaattgctat
     6061 tcataatcca gaagattttt ctaaattatg caaaagcgct gaaaaagcat taagtaaata
     6121 attgaaaaaa tagattattt attaatctat ttttttaaaa ttatatctat atattaataa
     6181 agcaaaatat gatataatat taataataag aagaggtgaa aactatgaga gagtttacgt
     6241 taaaagtaaa tggaaaagaa tatattaaaa aagataaagt ctacttaaga gatttagcga
     6301 aagaattaaa gatagaagct tatcttgcta aggtagatac tcgtcttcgt gagcttgatt
     6361 actatgtgaa ttatgatgcg gaagtcgaat ttttaaattt aacacatgta gagtcaatga
     6421 atgcttatga aactacttta cgttttctaa caatcatcgc tttagagaat ttatatcctg
     6481 gagttgaagt taagtttaga ttaggtgttt ctcgtagtac ctatggtgaa atcaaagaca
     6541 ttatggttga tgatgctcta cttgaaaagt tagaaaaaga aatgaaaaaa ctaattgaaa
     6601 aagatcaaga tattgttagg gtgaagttaa gtaaagaaga agcaaaagag atatatttaa
     6661 aacaaggata tcatgataag attgagactt taaaatatcg accagaagat acagttaact
     6721 tatataaatg taatggttac tataactatc tatttaacta tatgttacct tcaatgggtt
     6781 atattaaaga ttttaaatta attccttatt atcctggttt tataattctt tatccaagat
     6841 cagaacttgg tggtaaaatg ccggagtttc atgactcagt atcatttgcg aagatgttaa
     6901 gagcagcatc tcgttggtca aaacaattaa attgtgagac aattgctaat ctaaatactc
     6961 atgcaagtaa tgatagcatt attgatttag ttaatgtatg tgagactaaa cacaataata
     7021 tgttagctga acttggtgat aaaattaaat atgatattga aaacattaga ttaattgcga
     7081 ttgctggtcc atcatcttct ggtaagacta ctttctctaa taggctaaga ggggtattga
     7141 tggctaaagg tattgaacct ttaaagattt ctatggatga ttattataag ccaagaggca
     7201 aggctccttt agatgaaaat ggggaactag acttagaaca tattgaagca ttagatattg
     7261 atcgttttaa ccgtgatctt ttaaacttaa ttcaaggtaa gaaagttaca ctgccacact
     7321 ttgattttaa taaaggttat agtgttgatg gtaaaactat taaaattagt aaatcaacac
     7381 caattattat tgagggaatt cacgcattaa atgaaaaact tactagttta attcctagac
     7441 atcaaaagtt taagatttat attagtcctt tatttgaaat tagttatgat aatcatacac
     7501 ctattagtgc tacagaaatt aggcaattaa gaagagttgt tagagatgca aaatatcgtg
     7561 gtactaatgt tcgtgatact tttaaaatgt ggccttcagt aagaaagggt gaatttagat
     7621 ggatttatcc ttatcaagaa gaagctgatt atatttttaa tactgagctt acttatgaat
     7681 taggagtaat gaaaaaatat gctttaccag ttttaaatga agttccaaga gaagatgaat
     7741 attttattga tgctaacaga ctagttaaat tcttaaaact tgtaaaggat attcctgatg
     7801 aatatgtacc atgtaactca ctattaagag agtttattgg gggaagtatt ttttatagct
     7861 atgaaagaaa agaacaagaa gacttacttt cttaagcaaa ataatggctt attaactgct
     7921 ggttcaattg ataactttaa tacaatgact attggttgga gccactatgg ctatgttttt
     7981 ggactagaga ctattattgt ttatgtaagg aagagccgtt atacttttga ttttatttct
     8041 aaaaatgatt attttacagt atctttttat agtgatgaac ataaagatga tttagagtat
     8101 ttaggtacta aatcaggaag agatgaagat aaagtttcca agactagtct tactccagtt
     8161 aagtattttg actcagtaag ttttaaggaa gcaaaggcta cattttattt aaagaaaatt
     8221 tatcaaggtg agatatttga agatcagtta tcatcacctg taaaagagaa gtattttgta
     8281 gagggaaaca atcattattt atttatagga caggtgattg aaaaatgaaa tatatactag
     8341 caatagatca aggaactaca agttcaagag caatcctttt tgataagaag gctaatattg
     8401 tagcatcatc acaactagaa atatcttcaa ttacgaataa atctggttgg gtagaacaag
     8461 atcctttgga acttgtagaa tcagtaagac aagttattat tggtgtttta aataaagcta
     8521 atattagtgc taaagatatt gaagcagtag gtattactaa ccaaagagag acaactattc
     8581 tttgggataa aaaaacaggt aagccagtaa ataaagctat tgtttggcaa agccgtcaat
     8641 cagaaaagat ttgcaaagag tggattgaaa aaggttataa tgataaagtt ttagagaaaa
     8701 caggattaat tattaaccct tacttttctg cttcaaaaat caaatggatt ttagaaaata
     8761 ctaaaggttt agaagataaa ttaagtaaag gtgaaatact atttggtaca gttgatactt
     8821 atttaatttg gaatttatct aaaggaaagc ttcacataac tgattatact aatgcttcaa
     8881 gaacaatgct ttttaatatc aaagagttaa aatgggatga tgagttatta gaaaagtttg
     8941 ctttaccaaa atcaatttta cctaaagttg taccatcttc tggtaactta gggaaaatag
     9001 agattttagg tgaagaagtt aatttgcgag ctattgctgg ggaccaaagc gcagctttat
     9061 ttggtcaatg ttgttttgat ttaggtgatg taaagaacac ttatggtact ggatgttttg
     9121 ctttaatgaa tacaggaagc gaagttatta cttctaaaaa tggtttatta tcaactattg
     9181 cttggcatat tgatggtaag actacttatg ctttagaagg atctgtgttt gtagctggag
     9241 ctgctgttca gtggttaaga gatagtatga aatttttt
//
LOCUS       DAOSGS010000026         9253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6356_length_9253_cov_4.573386, whole genome shotgun sequence.
ACCESSION   DAOSGS010000026 DAOSGS010000000
VERSION     DAOSGS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6356_length_9253_cov_4.573386"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..595
                     /locus_tag="PLQ22_01965"
     CDS             152..595
                     /locus_tag="PLQ22_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58760.1"
                     /translation="MDLKPLCQRVADRLGIDPLEIKFEEMIDDSRLYIKEEYVAINKK
                     FENDYVECAKAITHEYRHVFQIFYANMFNDDRARRWRVELSNLVNTSNINNKGSNYIG
                     QELELDAFSFTKYYLDKYESMKVENKINGYDEIIEKYISINYNFL"
     gene            617..3538
                     /locus_tag="PLQ22_01970"
     CDS             617..3538
                     /locus_tag="PLQ22_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH58761.1"
                     /translation="MKAWLITINPDKWTWPDFDNVVKNTKDGVSKYKVDWTCSNTHVQ
                     IGDRVFLMRQGNYKKGIMAAGHATKASFLAPHFDPSKSATGQTVPHIEVEFDYILDPS
                     YDDLIDLNKLKSELPNQTWSPQSSGIEIVEPVYPELEYMWTMHIGISKNIYNLLKAQK
                     DINPDSHDGSYELIRTVIQKYSEMNSDTGLDSLDYNDLNLVYLATIGTWADGLDKKKE
                     HVKSSNLPQTAKNDLLLILDNLWENAKNSTYSNSKPDNPSLGMFGSGFFSFKSKVAID
                     TPKKILDLLVIMSSLTSDEEIITKSLELGNLDLSGLGLASISEILHCLWPNVFPISNN
                     RVNHIFDLLGFSLNNSGSISAYLENVSKIKSFRDFNFEFKNYRVLDIVAQRLKTTDID
                     YISVLEYLENNRSIPFEKITAGMAQTRIDELNSIKDKGRKAVAEMKKMAKLCADKFGL
                     DHCSGNKWLDGSNTKTRNYLWTILKYSAYENKPETISLFVDVDDKNVVRYRFSLEIQN
                     DKATTEDFNNHHKFLDYSVPQDSNLVYVTGSDHSDNIATSTKNAQELKEQLADGTISK
                     VQICRIVNKRAGLTSEECEQEMLEAVKNLLPFYEIAIGKESSKPIQNETIEIKRDVIM
                     DTTNNRLELNTILYGPPGTGKTYNTKRYAVAICDFGGDVETCKSAMPNYKDVESRYEQ
                     LEEDNRIAFTTFHQSYGYEDFIEGIKPVSKIDTAGNHYVDYPIQPGSFKVFCNEATRF
                     EMNKENCVFIIDEINRGNISKIFGELITLIESTKRINADESTFLQLPYSKESFGVPKN
                     VYILGTMNTADRSIALMDTALRRRFNFIEMMPETSVLNELGANKVVFNGKELDVVKML
                     NTINDRIEVLYDRSHTIGHAFFTSLAKDSSLTNLRLIFENKVIPLLQEYFYDDYEKIQ
                     LVLGDNAKTDNRFKFIIDETNRASSIFKGSVDENNIPEKKYKIQKEAFKYIESYLEII
                     K"
     gene            3552..4883
                     /locus_tag="PLQ22_01975"
     CDS             3552..4883
                     /locus_tag="PLQ22_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HOH58762.1"
                     /translation="MKKILEVKEFDKITGNPDYKEDSNYKYLEEPAFQNLIDFIKEYV
                     ASDNNDDAFDCFKVITQRGSGDLVITKNYVGLIQMKNGYQVEILPKISMGGVDDTNNR
                     VTKSTFIKMLRCMDDFQSKVFSSANLNIDRMNLYEIFINMYLQELNSLVKKGLKSTYI
                     TEEDNLSSFKGKLIIKDQIIKSITHKEKFSVVFDEYQINRPENQLIKSTLLKLNRITE
                     RIENKKNISQLLTYFERVDESINYVSDFSKVIIDRTMTDYDLLLKWSRIFLMNKSFTT
                     FSGETVSRSLLFPMDKLFEGYVSKNMKKIFAEYNWKVSTQDKGYYLFNEPNDQFALRP
                     DIVVTKQDGTLVIMDTKWKSLNNNARENYNISQADMYQMYAYARKYEKANNGIIPEVW
                     MLYPLNDEMRNHDKISYTSGDGVKVHIYFIDLTDIKMSIEKLKIYLIEGTQ"
     gene            4880..5338
                     /locus_tag="PLQ22_01980"
     CDS             4880..5338
                     /locus_tag="PLQ22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58763.1"
                     /translation="MIWEIRYYKNEINYKSKIHDFVETATGNKEFVIKSAKEKMARHN
                     YKIYELVHIKAELYNVKAENGDRFILRAYILSSGELQLEGQDFCESAEDLYGDEEYEY
                     YYLFDLQNTNKLKSILGVNDLIDSLVEFYKDEMSSKKLLELCDDNEIKYI"
     gene            5548..5931
                     /locus_tag="PLQ22_01985"
     CDS             5548..5931
                     /locus_tag="PLQ22_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH58764.1"
                     /translation="MSHINSCYDEIRISVEELKEYQNHRVYGETLWVAIKDNRNNKIV
                     ATGIGEMDNIVREGILEWIQISKEYRRQGFGKYIVKELFHRMKDKAKFVMVSGKLLSK
                     TNPLSLYEKCGFINKQIWNVYKINK"
     gene            6115..6525
                     /locus_tag="PLQ22_01990"
     CDS             6115..6525
                     /locus_tag="PLQ22_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58765.1"
                     /translation="METKEKNSRQGNKRIALLLVLKALEQMSDEFHPIKQVTLAKMVN
                     DIGRTLNLDIWCDRKTIGRHLKMLGAVGYKIIALKGKGCYLESSKFTKEESEIITRLI
                     TDSQLTDDKKSLLSEKILLQQRNINIKTFSNNLK"
     gene            6706..7491
                     /locus_tag="PLQ22_01995"
     CDS             6706..7491
                     /locus_tag="PLQ22_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOH58766.1"
                     /translation="MFDPKLEIGQVINNEELCQIFKCSSQGGMRRSITTGTLVLIANY
                     VNGIYHDRWIGGVLHYTGMGLNGDQDIDYSQNHTLNNAKLLGIDVFLFEVMEEREYTY
                     CGRAELVDEPYTEMQLDASGRNRKVCIFPIKPIPDNNVVKPPHYVFADMEDFKKRGQN
                     VDIEYADYRKKIKEHPLPKPIVIIPPVKPVKDATKILQEHDWLKGKYIKHSKYGEGII
                     KKIECNDNMEIVIGVKFDNETNGVIERTFKYKLCIENDWIELL"
     gene            7609..8874
                     /gene="rpoD"
                     /locus_tag="PLQ22_02000"
     CDS             7609..8874
                     /gene="rpoD"
                     /locus_tag="PLQ22_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOH58767.1"
                     /translation="MNYENPELELIVEEGKKKGYVALKSILAVVDEESEAFDEVTKEL
                     EGLNIDIVRDADLEAYESNDIDIDSDKFVEDFDGFIGELEGDPEFTEFTNEIENDLKT
                     KDIEDIVSSIVTHSQADDPVKMYLKEIGQVPLLNTTRELELSRIVQQGLIAKDIMEMV
                     EHGRKEVTDEEKGKLNLCIDEGEEARAIIIESNLRLVVSIAKRYLGRGMQFQDLIQEG
                     NMGLMKSVTKFDPTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRT
                     QRKLTQDLRREPTPEEIAAEMKITVEKVQQIQKIAQEPTSLDTPVGPEDDSTLGDFVS
                     DTEQPNPLDFTINEKYKEEIDVVLKTLTPREEKVLRLRFGLTNGKPHTLEEVGKEFGV
                     TRERIRQIEAKAIRRLRHPTRQKRLSQFK"
BASE COUNT         3622 a         1125 c         1664 g         2842 t
ORIGIN      
        1 agttcactag tggaactgag ataatagtat aatttgaaca gctactcagc cgagtggctt
       61 cttttttatg ttttattgtt tatctatagc aagtgtgata taatttattt aagaatgatt
      121 attcttttta attttgagag gttagataca aatggattta aaaccattat gccaaagagt
      181 agctgataga ttaggtattg atccattaga aataaagttt gaagagatga ttgatgatag
      241 cagactttat ataaaggaag aatatgttgc aataaataag aaatttgaga acgactatgt
      301 agagtgtgct aaagcgataa ctcatgagta tcgacatgtg ttccaaatat tctatgctaa
      361 catgtttaat gatgatcgtg caagaagatg gagagtagaa ctttctaatc ttgttaacac
      421 atcaaatata aacaacaagg gtagtaacta tatcggccaa gagctagaat tagatgcctt
      481 ttcttttaca aagtactatt tagataaata tgagtcaatg aaggtagaaa ataagattaa
      541 tggatatgat gagataattg agaagtatat atcgataaat tataattttt tatagatctt
      601 gaatggaggt ataaatatga aagcatggct aataacaatt aatcctgata aatggacatg
      661 gccagatttt gataatgtag ttaaaaacac aaaagacggt gtttctaaat ataaagttga
      721 ttggacatgc tctaatacac atgttcaaat aggtgatcga gtttttttga tgcgacaagg
      781 aaactataaa aaaggaataa tggcagctgg acatgcaaca aaagctagtt ttttagctcc
      841 tcattttgat ccttcaaaat cagctaccgg acaaactgtt ccgcatatag aggtggaatt
      901 tgattatata ttagatccat catatgatga cctgattgat ctcaataaat taaagtctga
      961 attacctaat caaacatggt ctcctcaaag ctctggtata gaaatagtcg agccagtata
     1021 tcctgaactt gaatatatgt ggactatgca tataggtata tcaaaaaata tttataattt
     1081 acttaaagct cagaaagata ttaatcctga tagtcatgac ggtagttacg aacttatcag
     1141 aactgtcatt caaaaatatt cagaaatgaa tagcgatact ggtctcgatt cactagatta
     1201 taatgattta aacttagtat atttggctac aattggtaca tgggcagatg gattggataa
     1261 gaaaaaagaa catgtcaaga gttccaattt gcctcaaact gcgaaaaatg atttgctatt
     1321 aattttagat aatctttggg aaaatgctaa aaattcaaca tactcaaata gtaaacctga
     1381 caacccatct ctaggtatgt ttggttcagg attcttttcg ttcaaaagta aagttgctat
     1441 cgatacgcct aaaaagatat tagatctatt agtaataatg tctagtctta cttctgatga
     1501 agaaattata acaaaatcac tcgaacttgg aaatttggat ttgtcaggat tgggattggc
     1561 gtctatatca gaaatattgc attgtttatg gcctaatgtt tttcctattt caaataatag
     1621 agtaaatcat atttttgatc tattaggttt ttcattaaat aattcaggca gcatctcagc
     1681 ctacttagaa aatgtttcta aaataaaatc atttagagat ttcaattttg agtttaaaaa
     1741 ttatagagta cttgatatag tggctcaaag attaaagaca acagacattg attatatttc
     1801 agttttagaa tatttagaaa ataacagatc aattccattt gaaaaaataa ctgctggaat
     1861 ggctcaaact agaattgatg aacttaacag tataaaagat aaaggcagaa aagctgttgc
     1921 tgaaatgaaa aaaatggcca aactttgtgc tgataaattt ggcttagatc attgcagcgg
     1981 aaataagtgg ttagatggtt caaacacaaa aacacgtaat tatttatgga ctattctaaa
     2041 gtattctgca tatgagaaca aaccagaaac tatctcattg tttgtagatg ttgatgataa
     2101 aaatgttgta agatatagat ttagtttaga aattcaaaat gataaagcaa caacagaaga
     2161 ttttaataat catcataaat ttttagatta ttctgttcca caagatagca atcttgttta
     2221 tgttacaggc tcagatcatt ctgataatat tgccacttcg actaaaaatg cgcaagagtt
     2281 aaaagaacaa ttagctgatg ggactatatc aaaagttcaa atttgtagaa ttgttaataa
     2341 gagagcaggt ttaacaagcg aggaatgcga acaggaaatg cttgaagcag tgaaaaactt
     2401 gcttccgttt tatgagattg ctataggaaa agaatcttca aaaccaatac aaaatgaaac
     2461 catagaaatt aagagggatg taattatgga tacaaccaat aatagattag aattaaatac
     2521 aattttatat ggtcctccag gaaccggaaa gacgtacaac acaaaaaggt atgctgtagc
     2581 aatttgtgat tttggaggag atgttgaaac ttgtaagtct gcaatgccga attacaaaga
     2641 tgttgaatct agatatgaac aattagaaga agataataga attgctttta caacatttca
     2701 tcaatcatat ggatatgaag attttatcga aggaattaaa ccagtaagta aaattgatac
     2761 cgctgggaat cattatgttg attatccgat tcaacctggc tcgtttaaag tattttgtaa
     2821 tgaagctact agatttgaaa tgaataaaga aaactgtgtc tttatcatag atgaaataaa
     2881 tcgcgggaac atatcaaaaa tttttggtga gttaattaca ttgattgaat cgaccaaacg
     2941 tattaatgca gatgaatcaa catttcttca attgccatat tcaaaggagt cttttggtgt
     3001 tcccaaaaat gtatacatac ttggtacaat gaacactgct gatagatcta ttgctttaat
     3061 ggatacagca ttaagaagaa gatttaattt tattgaaatg atgcccgaaa catctgtact
     3121 taatgaatta ggtgcgaata aagttgtatt taatggtaaa gaacttgacg ttgtaaagat
     3181 gcttaataca ataaatgata gaattgaagt tttgtatgat agatcacata ctattggtca
     3241 tgctttcttt acttctttag caaaagatag ttcattaacg aatttaagat taatatttga
     3301 aaataaggtg atacctttac ttcaagaata cttttatgat gattatgaaa agattcaatt
     3361 agtactaggt gataatgcaa aaactgataa tagattcaag ttcataattg atgaaactaa
     3421 tagagcaagt tctattttta aaggctcagt agatgaaaac aatattcctg agaaaaaata
     3481 caaaatacaa aaggaagctt ttaaatatat agagagttac ttagaaataa ttaaatagga
     3541 aggtaatctc tatgaaaaaa atattagaag ttaaagagtt tgataaaatc actggcaatc
     3601 cggattataa agaagattca aattataaat atttagaaga accagcgttt caaaatctaa
     3661 ttgacttcat aaaggaatat gtagcttctg ataataacga cgatgcattt gattgcttta
     3721 aagtaataac acaaaggggt tctggtgact tagtcataac aaaaaactat gttggtctaa
     3781 ttcaaatgaa gaatggatat caagtagaaa ttttacctaa gatatctatg ggtggtgttg
     3841 atgatactaa taatagggta acaaaatcaa cttttattaa gatgcttaga tgcatggatg
     3901 attttcaaag taaagttttt agctcagcaa acttgaatat cgatagaatg aatttatatg
     3961 agatattcat taacatgtat cttcaagaat taaacagttt agtaaaaaaa gggttaaaat
     4021 cgacatacat aactgaagaa gacaatttgt cttcctttaa gggcaagcta ataattaaag
     4081 atcaaataat caaaagcatt acacataaag aaaaattcag tgttgttttt gatgagtatc
     4141 aaataaatcg acctgaaaat caactgataa agtcaactct tttaaagctt aatagaatta
     4201 ctgaaagaat tgaaaacaaa aagaatataa gtcaactact tacttacttt gaaagggtag
     4261 atgaatctat aaattatgtt tctgattttt caaaagtaat aattgatcgt actatgactg
     4321 actatgattt gttattgaaa tggtcaagaa tatttttgat gaataagagt ttcacaactt
     4381 tttcagggga aacagtttct cgttccctat tgtttcctat ggacaagctg tttgaaggtt
     4441 atgtttctaa aaacatgaag aaaatttttg ctgaatataa ttggaaggtt tctactcaag
     4501 ataaaggtta ttatttattc aatgaaccta atgatcaatt tgccttaaga ccagatatag
     4561 tggttacaaa acaagatggg actttggtta ttatggatac aaaatggaaa agccttaata
     4621 ataatgcgag ggaaaactac aatatttcac aagcagatat gtatcaaatg tatgcttatg
     4681 ctagaaagta tgaaaaagca aataatggaa ttatacctga agtatggatg ttgtacccat
     4741 taaatgatga aatgagaaat catgataaaa taagttatac aagtggtgat ggtgtaaaag
     4801 tccatatcta ttttattgat ttaaccgaca ttaaaatgag tatagaaaaa ttaaaaatct
     4861 atctaattga gggaacacaa tgatatggga gattagatat tataaaaatg aaatcaatta
     4921 taaatctaag attcatgatt tcgtggaaac agcaacaggt aataaagagt ttgtaatcaa
     4981 aagtgcaaaa gaaaaaatgg caaggcataa ctacaaaatt tacgaattgg tacatatcaa
     5041 agcagagtta tataatgtaa aagcagaaaa tggagatcga tttattttaa gagcttatat
     5101 tctttccagt ggagaacttc aattggaagg acaagatttc tgcgaatcag cagaagattt
     5161 gtatggtgat gaagaatatg aatattacta tctatttgat cttcaaaaca ctaataagtt
     5221 aaaatcaatt ttaggtgtaa atgatctgat tgactcatta gttgagtttt ataaagatga
     5281 aatgtctagt aaaaaattat tagaactttg tgatgacaat gagataaagt atatataaaa
     5341 gatccttgta aagcttcaag tcttccttac tggaaaacta tatcttatca aatccctaat
     5401 aatattaaag ttgaattaga gaaatataat aaggaatcaa atgaagcatg tgaaaagtat
     5461 tttaaagtca tccattattt agataatata aaaacttcag ttttaccaat tgactttata
     5521 ctaactagta tttctatcga agaatatgtg agtcatatta attcttgtta tgatgaaatt
     5581 agaatatcag tagaagaatt aaaagaatat caaaatcata gagtgtatgg tgaaacttta
     5641 tgggttgcta ttaaagataa tagaaataat aagattgttg ctacagggat tggcgaaatg
     5701 gataacatag taagagaggg tattctagaa tggattcaga tttcaaaaga ataccgcaga
     5761 caagggtttg gtaagtatat agttaaggaa ctcttccata gaatgaaaga taaagctaag
     5821 tttgttatgg tatctggaaa actattaagt aaaacaaacc cactttcttt gtatgaaaaa
     5881 tgtggtttta taaataagca aatatggaat gtttataaaa ttaacaaata attattgcac
     5941 tagacgttta gcttgggtac agatttacat tgaaggtaat taaccaaagt aaagatataa
     6001 aacttaaact atctttttaa gtatttattg aatttaaaat caatgaatga taaaatcgtt
     6061 ttatgggcta atattggggc tttgtatatg atataatatt aatagggggt ttaaatggaa
     6121 acaaaagaaa aaaatagtcg acagggaaat aaaagaatag cattgctact tgtactaaaa
     6181 gcactggaac aaatgagtga tgagtttcat ccaataaagc aggtaacact cgctaagatg
     6241 gtaaatgata ttggaagaac tttaaatctt gatatttggt gtgatagaaa gacaattggt
     6301 agacacctta aaatgcttgg ggctgtagga tataaaataa ttgctctcaa agggaaagga
     6361 tgctatcttg agagtagtaa gtttacaaag gaagaaagtg agataataac tcgattgata
     6421 actgactcac aattaacaga tgacaaaaaa tctttgttat cagaaaagat tttattgcaa
     6481 caacgaaaca ttaatataaa aacattttct aataatttaa aataactaat ttaaatcagt
     6541 agtttaacct tctagtggta ttataaattt tgtatagaaa aaatataata tataaaaata
     6601 aaaatgcagt attaatcaaa aaccataaaa tagttgagtg gtaagtttta gaatataaac
     6661 actcttctat taatgtaaga tatttaagta aacggaggaa gaataatgtt tgatccaaag
     6721 cttgaaatag gtcaagttat aaataatgaa gaattatgtc aaatctttaa atgcagctca
     6781 cagggaggta tgagaaggtc aataacaaca ggaacgctag ttcttattgc aaattatgtt
     6841 aacggtatct atcatgatag atggattggt ggagtattgc actatacagg tatggggctt
     6901 aacggcgatc aagatatcga ttatagccaa aatcatacac tcaataatgc aaagctgctt
     6961 ggtattgacg tgtttctttt tgaagttatg gaagaacgag aatatacata ctgtggcaga
     7021 gctgaactgg ttgatgagcc gtacacagaa atgcaactag atgcaagcgg taggaatcgt
     7081 aaagtatgta tattccctat taaacctata ccagataata atgtagtaaa gcccccccat
     7141 tacgtttttg cggatatgga ggattttaaa aaaaggggtc agaatgttga tatagagtat
     7201 gctgactata gaaaaaagat aaaagaacac ccgctaccaa aaccaatagt aataattcca
     7261 ccagtaaagc cggtaaaaga tgcaacaaag atattacaag aacatgattg gcttaaggga
     7321 aaatatataa aacatagtaa gtatggcgaa ggaataatca aaaaaataga atgtaatgac
     7381 aatatggaaa ttgttattgg tgtaaaattt gataatgaaa caaatggagt tattgaaaga
     7441 acattcaaat ataaactctg tatagagaac gattggatag aattattata attatagttt
     7501 aaatagtaat ttgagaatac agtcaattaa tatgaatgac aaatttatag aatgcttaaa
     7561 gtaattgaag aaaatcacta tgaggcaatt gaaaatgggg ataattgaat gaactatgaa
     7621 aatccggaat tagaattaat tgttgaagaa ggaaagaaaa agggctacgt tgccctgaaa
     7681 agtatccttg cggtagttga tgaggaaagt gaagcttttg atgaagtaac aaaagaatta
     7741 gaaggtttga atattgatat cgttcgtgat gctgatttgg aagcttatga atctaatgat
     7801 attgatattg actctgataa gtttgttgaa gattttgatg gtttcattgg tgaattagaa
     7861 ggggatcctg aattcacaga atttaccaat gaaattgaaa atgatttgaa aacaaaagat
     7921 attgaagata tcgtatcttc aatcgttacc catagtcaag ctgacgatcc agtaaaaatg
     7981 tacttaaagg aaattggtca agtaccacta ttaaatacaa cgagagaatt agaattatcg
     8041 cgtattgtcc aacaaggttt aatcgccaaa gatattatgg aaatggttga acatggacgt
     8101 aaagaagtta ccgatgaaga aaaaggaaaa ttaaatttgt gtattgatga aggcgaagag
     8161 gcacgagcta tcattataga atctaacttg cgattggttg tttccatcgc caaacgatat
     8221 ttgggtcgtg gaatgcagtt tcaagattta attcaagaag gaaacatggg attgatgaag
     8281 tcagtgacta aatttgatcc taccaaggga tttaaatttt caacctatgc aacatggtgg
     8341 attcgtcaag ccattactag agccatagcc gatcaagcac gaaccattcg tatccctgtt
     8401 catatggtag aaacgattaa taaattagtt agaacacagc gaaaactaac acaggatctg
     8461 cgtcgggaac ctactccaga agaaatcgca gctgaaatga aaattactgt tgagaaagta
     8521 caacaaatac aaaaaatcgc tcaagaacca acttctttag acactccagt tggtccagaa
     8581 gatgattcaa cgctaggcga ttttgtcagt gatactgagc aaccaaatcc attagatttt
     8641 acgatcaatg aaaaatataa agaagaaatt gatgtcgtat taaaaacact aactccacgt
     8701 gaagaaaaag tgttgcgttt acgtttcggt ttgaccaatg gaaagccgca taccttagaa
     8761 gaagtgggaa aagaatttgg agtgactcgt gaacgtattc gacaaataga agctaaggct
     8821 attagaaggc ttcgtcatcc tactagacaa aagagactga gtcaatttaa ataaatagca
     8881 gatagtttat ctatctgctc aggatgttga caaactacct tttttgaaaa acaataaaag
     8941 gtagtttttt attttctcca tttgacactt aaatatacac ccaatatgat gattaatccc
     9001 aacttaataa ttttatggga ataaaaaaat gttagcgttg acaaagagct ttccccaaaa
     9061 tactttaaaa taataaaagt aactatgctt acgaagaagc tcattatcat agcttttagt
     9121 tgctaaaaaa gagatctatc aaggtgaatg gattaagaaa attgaaatag aattagtatt
     9181 aaataaggta tatcaatata gatcttaaac aatatattaa tacctatgga ctccataaca
     9241 taaaagttat tgc
//
LOCUS       DAOSGS010000027         9186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6414_length_9186_cov_5.258460, whole genome shotgun sequence.
ACCESSION   DAOSGS010000027 DAOSGS010000000
VERSION     DAOSGS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_9186_cov_5.258460"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLQ22_02005"
     CDS             complement(<1..589)
                     /locus_tag="PLQ22_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58768.1"
                     /translation="MLFLKELKNKSLYKKLLNNYFLLALGIVLILLSLKYYLVILVTI
                     VYLIYFYRKSKELFYVVVGLALIIILIYFLKLKITLRDHNYLEGQIINIKKGDISQTL
                     TVKKSWFKIMVYTDLETKYSVLDYIKFQGEEVIIDDVRVPYDFDYKEYLKHQGISKVI
                     SATSIVKIKKRFSYLYLRELIFILTIEVALITLDIP"
     gene            complement(549..1079)
                     /locus_tag="PLQ22_02010"
     CDS             complement(549..1079)
                     /locus_tag="PLQ22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH58769.1"
                     /translation="MNKKKIIIIVSVVAFLVITGIVISLINKPKVENPIDDQREIIIE
                     NVVVEIKGEVYNPGLYILEQGSRINDLIELSGGFTKDANGTDLNLARVLSDGEVIIVS
                     KKGESGTIIESKIININYATVEQLMELSGIGETKAKAIVTYREQNGRFKKIEDIMNVS
                     GISNALFERIKEQITV"
     gene            1228..3432
                     /locus_tag="PLQ22_02015"
     CDS             1228..3432
                     /locus_tag="PLQ22_02015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH58770.1"
                     /translation="MEKVLKLLFLLFIALFLVSCKDEEEPQEESVTTLTLTTKNLTLE
                     EEETKQIEAVAKTDKGEAVAITYEVADPTVASVDSTGKVTGIKKGSTTITLKANDKTD
                     TCNVTVTEKEVVDPPIEPKVTFSVSEVTIDLDANPEYTLKPKLVGEAELLYAIDKPEI
                     ISIEGNKITALKTGTAIVTATYGKSTATVTVNVVKQFIVSLGEDEISEGAEVLAYVNE
                     ELALTIVDQTDIGLGVECSSSDKTIATITNKGVIMPLSEGTATITVQSGTTPELVRTF
                     QLVVGYKPLQSFTISYPDDMRTSGGPYDAIVELNPVSAKNDLKYELVEPSSAVSLRPL
                     NGQLTVNTNKGFAKIRAYSQSNPNIEAHVIVEIKLSLTDLINYLNQPLTVAQNVTLVG
                     WQTVNNPPHPVEPMYGSINKYLFDEHRVIYNWMQPGTMGYVEYETGSPNPVMFVTVHD
                     AGGNSPGETAQMLSNYCNSNAEVSYHYSVDDSEAWQNIEHGRAAWHAGCDQGLSGFSY
                     LDTGVPYDITRPRPTVDVSTDNYLLIDGKKSLIQVPSAGGRQATKNDFPSIGLMFFQR
                     NGNYYTTTLYYNSTYRTISNRGGNDLTVGIESSINQGGDLYQTWQRLAKLVANILHEH
                     NLGLDAVSQHNNWSGKDCPGTMRRAGYWDHFMGLVEAELLVLQELSEYEITLELVNDA
                     DKEYVSARGRVLKLPNARRTIQYNFKLRKEGVSYSFPLTSTIVAATEIRPAI"
     gene            complement(3511..5718)
                     /locus_tag="PLQ22_02020"
     CDS             complement(3511..5718)
                     /locus_tag="PLQ22_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOH58771.1"
                     /translation="MNMVRGAISRVIYFKNNFGIVQIMLDHNDPAMKEIIEEQYTLTI
                     TITSNFDRKPFKDELYEFTGKFKDTDYGYQFQANHFERIMANTLEGIVNYLSSDLFSG
                     IGKQKATRIFNTLGSDCLNVIVSDPKSLDKVKGLTSNNKEAIVKVLQENAYSRKTTVA
                     FLNLGLTMTAALKLINAYGNDAYEIVKANPYILIDEVEGYGFKRADQIALSLGFEENS
                     SLRLKALVMYLLKELTYSFGNTYFNKEDLYERVNNELKTWETSLKEFESFLDELNNEK
                     KIIIEDKDIFLKKVYDSEKSFALKIKELNAKEVSDIDTEDLIKQAEKKFGLTYGKEQK
                     EAISNALLNKVSIITGGPGTGKSTIIKGIIYCFQKQFKASDLSIAQLAPTGRAAKRMQ
                     EITGKDAMTIHKFLGYEGGDIFRYGEDALIDSELVIVDEFSMVDIELANRLVSALASN
                     TRLVIVGDADQLPSIGPGDVLNDLIKSDYFKVTKLHDIYRQEEGSAIVNLAHSINEGY
                     LPEYFRENSSDWSFISLEKDQIINGIIEVVERALNKGMDLVKDIQVLVPMYRGENGIN
                     NINNALQEKFNPLKDEEIKSHNHSFRIGDKVLQLVNRSEKDIMNGDIGKVYRFEKSDG
                     EITGLEVEFDSGIVKYKLEELDDLTLAYAITIHKAQGSEFDLVVMPITSQYYIMLRKK
                     LIYTGITRAKKYLVMLGSVNYLAMGITKMDDQRQTKLKERLEDKREVTPFDFM"
     gene            complement(5718..6827)
                     /gene="mnmA"
                     /locus_tag="PLQ22_02025"
     CDS             complement(5718..6827)
                     /gene="mnmA"
                     /locus_tag="PLQ22_02025"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH58772.1"
                     /translation="MKKKVILGFSGGVDSAVSAIILKEQGYDVEGMFMRNWDSALNYD
                     ILGNPNNPDDICPQEQDYNDAVEAARIIGIKLHRVDFVEEYWNKVFMHFIDEYKQYRT
                     PNPDILCNSEIKFKCFVEKATEMDADYIAMGHYAKITHKPLKLYRGKDRNKDQSYFLC
                     MLTKKQLEKALFPLGDLTKKEVREIARKYKLNIADKKDSTGICFIGERDFDKFLKNYL
                     PAQVGNMETLRGEVVGKHEGLMYYTIGQRKGLGLGGDKGPWFVLGKDIKRNVLLVGYQ
                     EDQEYLYSNRCFASSFNWLKEDFKEIEVLAQFRYRSKAVKAKVRRIDKDTIEVKYEPF
                     RAVTPGQAVVFYNNDEMLGGAVIDEVYQDDQRRLY"
     gene            complement(6836..8818)
                     /gene="uvrB"
                     /locus_tag="PLQ22_02030"
     CDS             complement(6836..8818)
                     /gene="uvrB"
                     /locus_tag="PLQ22_02030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH58773.1"
                     /translation="MMAKFILKSNYDPKGDQIKAIKELTDNLKAGMKEQTLLGATGTG
                     KTYTVANVIKNIGKKTLVLAHNKTLAGQLYSELKTFFPENRVEYFISYYDYYQPEAYV
                     AAKDLYIEKDSSINDDIDQMRHSAVSSLLTEDDVIVVASVSCIYGIGNPEDYLENMLT
                     LRQNDIYSRSDLMNKLVKMEYLRNDYEFKRGTFRVRGESIDIIPPHEKDEAVRIEYFD
                     NEIERILILDSLTGNKIKETKVINIFPATLFSTSSSKKEEGIRRIKLELEEQIANFQK
                     QGKLLEAQRIEQRTKYDLEMLEEIGSCSGIENYSRHLSLREEGETPYTLVDFFKKDFL
                     LIVDESHVTIPQVRAMYNGDRSRKLSLVENGFRLPSALDNRPLTFDEFNQKLDQVIYV
                     SATPAEYEKERSGDIVEQIIRPTGLLDPIVVVKPSEGQIEDLIKEIKNRIKQKERTLV
                     LTLTIKMAEDLTNYLKELDIKVAYLHSEIKALERLEIIRKLRLGVYDCLVGINLLREG
                     LDIPEVSLIAILDADKEGFLRSETSLIQTIGRAARNVNGMVILYADNITRSMENAINE
                     TYRRRDIQDRYNQEHNIIPQTIYKDIQDEFVITRELQEDLKTYDLNKLANLSTVERRK
                     VIKSLEDKMRQAAKDLDFEQAMVLRDIIFEIKAELE"
     gene            complement(8815..>9186)
                     /locus_tag="PLQ22_02035"
     CDS             complement(8815..>9186)
                     /locus_tag="PLQ22_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58774.1"
                     /translation="EEKTLMIATDTGIFPEKYTSLLEATDALIIEANHDIDTLINSDR
                     HQMLIDRILSAQGHMSNTATHYLLKQFLTERTKIIVLAHVSEECNSDDEIERDIIKPL
                     DFKGDIYIARQHQALPMIEVK"
BASE COUNT         3067 a         1595 c         1134 g         3390 t
ORIGIN      
        1 aaggaatatc taaagtaatt agtgcaactt caatagttaa aataaaaatc aattctctta
       61 agtataaata agagaatctt ttttttattt taactattga agttgcacta attactttag
      121 atattccttg atgttttaaa tattctttat aatcaaaatc ataaggaact cttacatcat
      181 caattattac ttcttctcct tgaaatttaa tataatctaa aacactatat ttagtttcta
      241 aatcagtata aaccataatc ttaaaccaag actttttaac tgttagagtt tgacttatat
      301 cccctttctt aatattgata atttgacctt ctaaataatt gtgatctctt aatgtaattt
      361 ttagtttaag aaaataaatc aagatgataa ttaatgctag tcctactact acataaaata
      421 attcttttga ttttcgataa aaatatatta aataaacaat agttactaaa ataactaaat
      481 agtattttag tgaaagtaaa attaaaacta ttcctaaagc taataagaaa taattattta
      541 agagcttttt atacagtgat ttgttcttta attctttcaa aaagagcatt ggaaatacct
      601 gaaacattca taatgtcttc aatcttttta aatctaccgt tttgttcgcg atacgttaca
      661 attgcttttg ctttagtttc accaataccc gatagttcca ttaattgttc tactgtagca
      721 tagttaatat taataatttt tgattctatt attgtcccac tttcgccttt tttagaaaca
      781 attataactt cgccatcaga taaaactcta gccaaattaa gatccgtacc attagcatct
      841 tttgtaaagc caccagataa ttcaattaga tcattaattc ttgaaccttg ttctaaaata
      901 tataaaccag gattataaac ttcaccttta atttcaacta caacattttc tataataatt
      961 tctctttgat catcaattgg attttctact ttaggcttat ttataagact aattacaata
     1021 cctgttatta ctaaaaatgc tactacacta acaataataa tgattttctt tttattcatt
     1081 gcttactcct tttacttata tattataaaa atgtaagccc ttttactttt atttttttcc
     1141 aaattatgct atactataaa agaagagata tctaatcaaa ttttatgata cgatatttaa
     1201 tactattttt gaataagagg aggtactatg gaaaaagtat taaaactatt atttttactg
     1261 tttatagctt tattccttgt tagttgcaag gatgaagaag aaccacaaga agaaagtgta
     1321 acaactctta cactaacaac aaaaaatctt accttagagg aagaagaaac aaaacagatt
     1381 gaagctgttg caaaaacaga taaaggcgaa gctgttgcaa taacttatga agtagctgat
     1441 ccaacagttg ctagtgttga tagcacaggt aaagttactg gaattaagaa aggaagcact
     1501 acaattactt taaaagctaa cgataaaacc gatacatgca atgttactgt aactgaaaaa
     1561 gaagtggttg acccaccaat tgaaccaaaa gtaactttct cagtttcaga agttacaatt
     1621 gatttagatg ccaaccctga atatacatta aaacctaaat tagttggtga agctgaacta
     1681 ttatatgcaa tcgataaacc agaaattatc agtatcgaag gcaataaaat cactgcccta
     1741 aaaactggta cagcaattgt tactgctaca tatggaaaat caactgctac agttactgta
     1801 aatgttgtga aacaattcat tgtaagttta ggtgaagacg aaatttctga aggtgctgaa
     1861 gttttagctt atgtaaatga agaacttgca ttaactattg tagaccaaac agatattggc
     1921 cttggtgttg aatgttcaag tagcgacaaa acaatagcta caattacaaa caaaggtgtt
     1981 attatgccac tttcagaagg cactgctaca atcacagttc aatcaggtac aactcctgaa
     2041 ttagttagaa ctttccaact tgttgttggt tataaaccat tacaaagttt cactattagc
     2101 tatccagatg atatgagaac aagtggtgga ccttatgatg ctatagtaga attaaatcct
     2161 gtaagtgcta aaaacgatct taaatatgaa ttagttgaac ctagtagtgc tgtttcatta
     2221 agacctttaa acggacaatt aacagttaat acaaacaaag gctttgctaa gattcgtgct
     2281 tactcacaat ctaatccaaa tattgaagca cacgtgattg tagaaattaa attaagtcta
     2341 actgacttaa ttaactattt aaatcaacct ttaactgttg ctcaaaacgt aacattagta
     2401 ggttggcaaa cagttaacaa tccaccacat ccggttgaac caatgtatgg tagtatcaat
     2461 aaatatctat ttgatgaaca tagagttatt tataactgga tgcaacctgg aactatgggc
     2521 tatgtagaat acgaaacagg atctccaaat ccagtaatgt ttgttactgt tcatgatgct
     2581 ggtggaaact cacctggaga aactgctcaa atgctttcta actactgtaa ttcaaatgct
     2641 gaagtatcat atcactattc agttgatgat tcagaagctt ggcaaaatat tgaacatggt
     2701 agagctgcat ggcatgcagg ctgtgatcaa ggattaagcg gattttcata tttagataca
     2761 ggagttccat atgatattac tcgtcctcgt ccaactgtag atgttagtac agataactac
     2821 ctactaattg atggtaaaaa gagtcttatc caagtaccat ctgctggtgg tagacaagct
     2881 actaaaaatg atttcccttc tattggatta atgttcttcc aacgtaatgg aaactactat
     2941 actactactt tatattacaa ctctacttat agaactattt ctaatagggg tggtaatgac
     3001 ttaactgtag gtattgaatc ttctatcaac caaggtggag atttatatca aacatggcaa
     3061 agacttgcaa aactagttgc taatatcctt catgaacata acttaggatt agatgcagtt
     3121 agtcaacata ataattggtc tggaaaagat tgtccaggaa caatgagaag agctggttat
     3181 tgggatcact ttatgggctt agttgaagct gaattacttg tattacaaga actaagtgaa
     3241 tatgaaatta cacttgaatt agttaacgat gcagacaaag aatacgtaag tgcacgtgga
     3301 cgtgttctaa aacttcctaa tgctagaaga actatccaat ataacttcaa gttaagaaaa
     3361 gaaggagtat catattcatt cccattaact tcaacaatcg ttgctgctac agaaattaga
     3421 ccagctattt aatgaaaaaa taataaaata aaaagagtga aaacgaaaat ttcactcttt
     3481 ttttatttta aaatcattta ttctttatgc ttacataaaa tcaaatggtg taacctctct
     3541 cttatcttct aatctttctt ttaatttagt ttgcctttga tcgtccattt ttgtaattcc
     3601 cattgctaga taattaacag aaccaagcat aaccagatat tttttagctc ttgtaattcc
     3661 tgtataaatt aacttctttc ttaacataat ataatattgc gaagttattg gcattactac
     3721 taaatcaaac tcacttcctt gagctttatg aatcgtaatt gcatatgcta gtgttaggtc
     3781 atctaattct tctaatttgt attttacaat cccagaatca aactcaactt ctaaaccagt
     3841 aatttcgcca tcactttttt caaaacgata aactttacca atatcaccat tcattatatc
     3901 tttttcacta cgatttacta attgtaagac tttatcccca attctaaaag aatgattatg
     3961 actttttatt tcttcatctt ttaacggatt aaacttttct tgcaaagcat tattaatatt
     4021 attaatgcca ttttctccgc gatacatagg cactaatact tgaatgtctt taactaaatc
     4081 cattccttta tttagagctc tttctacaac ctctataatt ccattaatta tttggtcttt
     4141 ttctaaagaa ataaaactcc aatcagaact attttcccta aaatattcgg gaaggtatcc
     4201 ttcattaata ctatgagcta agttgacaat tgctgatccc tcttcttgac gataaatatc
     4261 atgcaatttc gtaactttaa aataatctga ttttattaaa tcattcaaaa catctccagg
     4321 tccaatagaa ggtagttggt ctgcatcacc aacaatcact aatcttgtgt ttgaagctaa
     4381 agcagaaacc aatctattag caagttcaat atctaccatt gaaaactcat ctacaatcac
     4441 aagctctgaa tcaattaaag catcttctcc atacctaaag atatctccac cttcataacc
     4501 taaaaattta tgaatagtca tagcatcttt accagtaatt tcttgcattc tttttgctgc
     4561 tctaccagta ggtgctagtt gagcaatact taaatcactt gctttgaact gtttttgaaa
     4621 acagtaaata atacctttga taatagtgct tttaccagtt cccggaccac cagtaataat
     4681 tgatactttg tttagtaaag cattactaat tgcctctttt tgttctttac cataagttag
     4741 cccaaatttc ttttctgctt gtttgattaa atcttcagta tcaatatcac taacttcctt
     4801 agcatttaat tctttgattt tcaaagcaaa actcttttct gaatcataga ctttctttaa
     4861 aaagatatct ttatcttcaa taattatctt tttttcatta tttaattcat ctagaaaaga
     4921 ctcaaattct ttaagagatg tctcccaagt ttttaattca ttgtttactc tttcatataa
     4981 atcttcttta ttaaaataag tattgccaaa gctataagtt agttctttta ataagtacat
     5041 aactaatgct tttagtctta aagaagaatt ttcctcaaag cccaaactta aagcaatttg
     5101 atctgctctt ttaaagccat aaccttctac ttcatcaatt aatatatatg gattagcttt
     5161 tactatctca taagcatcat tgccataagc attgattaat tttaaagctg cagtcattgt
     5221 tagtcctaag ttaagaaaag caaccgttgt tttacgtgaa taagcatttt cttgcaagac
     5281 cttaactatt gcttctttgt tatttgaagt taagcctttt actttatcta aacttttagg
     5341 gtcactaaca ataacattta aacaatcaga acctaaagta ttaaatattc ttgtagcttt
     5401 ttgtttgcca attcccgaaa acaaatcact tgataaatag ttaacaatac cttctaaagt
     5461 attagccatt attctttcaa aatgattagc ttgaaactga taaccatagt ctgtatcttt
     5521 gaattttcca gtaaactcat ataactcatc tttgaaaggt ttacgatcaa agttagatgt
     5581 aatggtgatg gtaagtgtat attgttcttc aataatttct ttcattgctg gatcattatg
     5641 atctagcatt atttgaacta ttccaaaatt gtttttgaaa taaatgactc ttgaaattgc
     5701 gcctctgacc atattcatca atataatctc ctttgatcat cttgataaac ttcatcaata
     5761 acagccccac ctaacatttc gtcattgtta tagaaaacaa cagcttgacc aggagttaca
     5821 gctctaaatg gttcatattt tacttctata gtatctttat caattcttct aactttagct
     5881 ttaactgctt tacttcgata tctaaattga gctaatactt ctatttcttt aaaatcttct
     5941 tttaaccagt taaagctaga agcaaaacaa cgatttgaat ataagtattc ttgatcttct
     6001 tgataaccta caagtaatac atttctttta atatctttac ctaaaacaaa ccaaggtccc
     6061 ttatctcccc ctaaacctaa accttttctt tgaccgattg tgtaatacat taaaccttca
     6121 tgtttaccta caacttctcc tcttaaagtt tccatattac caacttgtgc tggaagataa
     6181 ttttttaaga acttatcaaa gtctctctca ccaataaaac aaattcctgt agagtctttt
     6241 ttatcagcaa tatttagttt atattttcta gcaatttccc ttacttcttt tttagttaaa
     6301 tctcctaaag gaaataaggc tttttctaat tgttttttag ttaacataca aagaaagtaa
     6361 ctttgatctt tattacgatc ttttccacga tatagtttta aaggtttatg agtaatctta
     6421 gcatagtgtc ccatagcgat ataatcagca tccatttccg ttgccttttc aacaaaacat
     6481 ttaaacttaa tttcgctatt acataaaata tcaggattag gagtacgata ctgtttatac
     6541 tcatcaataa aatgcataaa gactttattc caatactctt ccacaaaatc aactctatgc
     6601 agtttaatcc caataattct agctgcttct actgcatcat tatagtcttg ttcttgagga
     6661 caaatatcat ctggattatt agggttacct aaaatatcgt aattaagtgc tgaatcccaa
     6721 ttacgcataa acataccctc tacatcatat ccttgttctt ttaaaataat tgcacttact
     6781 gctgagtcta ccccaccact aaatcctaaa attactttct ttttcataat aacctctatt
     6841 ctaattctgc tttaatctca aaaataatat ctcttaatac cattgcttgt tcaaagtcaa
     6901 gatctttcgc agcttgtctc attttatctt ctaaagactt aataactttt cttctttcaa
     6961 ctgtacttaa attagctaat ttatttaaat cataagtttt taaatcttct tgtaattctc
     7021 ttgtaattac aaactcatct tgaatatctt tatagatagt ttgaggaata atattatgtt
     7081 cctgattata tctatcttgg atatctcttc ttctatatgt ttcattaata gcattttcca
     7141 ttgatctagt tatattatct gcatataaaa taaccatacc atttacattt cttgcagctc
     7201 taccaatcgt ttgaattagc gatgtttcac ttcttaagaa tccttcttta tccgcatcta
     7261 aaatagcaat taaagatact tcaggtatat ctaaaccttc tcttaaaagg ttaatgccta
     7321 ctaggcaatc atagacacct aatcttaatt ttcttattat ttctagtctt tctaaagctt
     7381 taatttctga gtgtaaataa gcaactttaa tatctaactc ttttaagtaa ttagttaagt
     7441 cttctgccat tttaattgta agagttaaga ctaaagttct ttctttttgt ttgattctat
     7501 ttttaatttc tttaattaaa tcttcaattt gaccttcact tggctttaca acaacaattg
     7561 gatctaaaag tccagttggt ctaataattt gttcgacaat atctccgctt ctttctttct
     7621 catattcagc tggagttgca cttacataaa taacttgatc tagcttttga ttaaactcat
     7681 caaatgtaag tggtctatta tctaaagccg atggtaatct aaagccattc tctacaagac
     7741 ttaactttct tgatctatcg ccattataca tcgctctaac ttgaggaata gttacatggg
     7801 attcatctac tattaaaaga aagtcttttt taaagaaatc aactaaagta tacggagttt
     7861 ctccttcttc ccttaaactt aaatgacgag aatagttttc aattccagaa caagaaccaa
     7921 tttcttctag catctctaaa tcatatttag ttctttgttc gattctttgc gcttctaaga
     7981 gttttccttg tttttgaaaa ttagctattt gttcctctaa ttctaacttt attcttctaa
     8041 taccttcctc tttttttgat gaacttgtag aaaagagtgt cgcaggaaag atattaataa
     8101 ctttagtttc tttaatttta ttccccgtta gactatctaa aattaaaatt ctttcaatct
     8161 cattatcaaa atattcaatt ctaactgctt catctttttc atgaggagga ataatatcaa
     8221 ttgattctcc tcttacccta aatgtgcctc ttttaaactc ataatcattt cttaagtatt
     8281 ccatcttaac tagtttgttc attaaatctg aacgagagta gatatcgttt tgccttaaag
     8341 taagcatatt ttctaaatag tcttctggat taccaatacc ataaatacaa gaaacagatg
     8401 caactacaat tacatcgtct tctgttaata atgaactaac agctgagtga cgcatttggt
     8461 cgatatcgtc attaattgat gaatcctttt caatatataa atctttagct gctacataag
     8521 cttcgggttg atagtaatca taataagaaa taaagtattc aactctattt tctggaaaga
     8581 atgttttgag ttcactatat aattgtcctg ctaatgtttt gttatgagct aaaaccaaag
     8641 tcttcttacc aatattttta attacattag ctaccgtata agttttacca gttcctgtag
     8701 ctcctaaaag agtttgttct ttcatcccag cttttaagtt atcagttaat tctttaattg
     8761 ccttaatttg atctccttta ggatcataat tgctttttaa aataaactta gccattattt
     8821 cacctctatc ataggtaatg cttgatgttg ccttgcaata tagatatctc ctttgaaatc
     8881 taaaggtttt attatatctc tttcaatttc atcatcacta ttacattctt cagaaacatg
     8941 agctaaaaca attattttag tcctttctgt aagaaattgt ttaagtaaat aatgtgtagc
     9001 tgtattgctc atatgccctt gagcacttaa aattctatca attaacattt ggtgacgatc
     9061 actattgatt aaggtatcaa tatcatgatt tgcttctatt attaaagcat ctgttgcttc
     9121 taataatgaa gtatattttt caggaaaaat acctgtatca gttgcaatca ttaacgtctt
     9181 ttcttc
//
LOCUS       DAOSGS010000028         9184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6416_length_9184_cov_4.098477, whole genome shotgun sequence.
ACCESSION   DAOSGS010000028 DAOSGS010000000
VERSION     DAOSGS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6416_length_9184_cov_4.098477"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="rsmD"
                     /locus_tag="PLQ22_02040"
     CDS             <1..490
                     /gene="rsmD"
                     /locus_tag="PLQ22_02040"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOH58775.1"
                     /translation="PMMDRMKESVFNTIDSYIEGANVLDLFGGSGALSLEAISRGAKH
                     SSISEINYEAYKVILENVQTLKEENSVTIYKMSYQKLLQVIKENEFKFDLVFVDPPYK
                     LEVANSIIEELIQSGNLNDEAIIVSHYYKGTYPVLTQENLEVLKHNVMGTSEYIIYKY
                     IN"
     gene            584..660
                     /locus_tag="PLQ22_02045"
     tRNA            584..660
                     /locus_tag="PLQ22_02045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:618..620,aa:Arg,seq:tct)
     gene            complement(727..1329)
                     /locus_tag="PLQ22_02050"
     CDS             complement(727..1329)
                     /locus_tag="PLQ22_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58776.1"
                     /translation="MRKTITLFILILLLPLLTSCKEKINSLEDFFTLLEKENYQLVTS
                     FIRQDESKITYAVELDVDKAKIAYDVYDSEGLKTFSQITYVAKENRSIFSYTYDEVKQ
                     TWTKQISNEDRLPGTLPKEWTNINNYSQYIKENQYMLKATFTNPEKYTYFYLTIENNN
                     VTGQGKTTLDDGSGAVTAIWKFSNYGKVVVNLPNAAKKAS"
     gene            1554..2435
                     /locus_tag="PLQ22_02055"
     CDS             1554..2435
                     /locus_tag="PLQ22_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58777.1"
                     /translation="MACKDKGAVAHDTHIVGEDWIVSSEEHWHGCTIAGCKEVFDKGK
                     HVYEEGSNVCSICGYKKGTTPSKPDSDPDQDPNTDPDPNTDPDPNTDPDPNTDPDPNT
                     DPDPNASVLDLLSSNFKMTINTVSEYENTVGELYRVGNDFMTVYKIYIEGYGTQITID
                     YYKYYAETETWTRYGATSGDTKWSEKVTNLSEDEVLYDSNLDLRFIINYPEKPDNLGY
                     VVNKGDKEDLYISDKKYECTIYTTPDNECQYHICVISGLQHTLKLKLYGAMITITEYK
                     MGGVSFPTFPGEIPNLP"
     gene            2448..4001
                     /locus_tag="PLQ22_02060"
     CDS             2448..4001
                     /locus_tag="PLQ22_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58778.1"
                     /translation="MKKRVSLLLLVFVSLLLTGCGSSTLNIKDIVEPQFVDFISEKKE
                     YSKPLTIETNEELDNNLSSKTIIGFKNKTFYNLETKKFIVFDRMPAGEIEYRVRDQYI
                     VMSTEQFHYIHNGKTGNYLFSLEKGDDVTVFFFASTIYYDYKDEQGNDKSDKYVFTDY
                     KYKKEEIVEEGDEAPNYDEINGDYGYIKIDDTKIKIFKDDRTYHVISFNDGYEIGYEI
                     DDWAWGDRIDGWYVLKNGNILIQRFKQVANDFPTFHFVDDGVRFVMKSEIFIVKSATL
                     KSIDLDFVVEDVNNGGFYKADNLVHLWRIDSILKDFTGEEYVFFDNEIKSEKRLEFKY
                     GNIDVTNLEIVGENLFIYNDETTIYALDGNNKVKGEKHYDADDYWFDPLLNGLIEFED
                     KTTSKCSLYNILDNKVLYEDLTKLVGAKQYALYRKDDGKIIMASLYGLVEMEYTDYRT
                     YGGSCYKLYTDDEKTFELFNIQGKSLGKFEDTTIQVISRDIGFGGEGYLAIRVEKGLE
                     SKVLVYKVK"
     gene            4086..4499
                     /locus_tag="PLQ22_02065"
     CDS             4086..4499
                     /locus_tag="PLQ22_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58779.1"
                     /translation="MNKYYELLKEKEYQKIKEEAIIKNDLDLDYLIVLISASFKTNEF
                     VILYYYLSKTNFKKEIEDGGANYTNYLNIIDEKQQLALVLTNFSLGVIKEKKEQEIDD
                     DYLNYRVIDLLNILFEIGYEDEVLNKIYHLLMSVE"
     gene            4569..5861
                     /gene="tig"
                     /locus_tag="PLQ22_02070"
     CDS             4569..5861
                     /gene="tig"
                     /locus_tag="PLQ22_02070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH58780.1"
                     /translation="MKIAVKKLEGSQAEVKVTLNKEEFSKYYDEAFDKVLADYQVEGF
                     RKGKVPRNIYLNRVGSDALVLEEAVNLSINGTYFEAIQEKKVRAVNYPKVDFDPNQVK
                     KDGGFTYTAVVDVYPEVELGEYFGIEYKEEKQEISKEELERVLRQERERKAVLVLKED
                     GCIELGNTVVFDFEGFVDGVAFEGGKAENYSLEIGSGQFIPGFEEQMLGMKEGEEREL
                     NVKFPDEYHSENLKGKDAIFKVKVHEIKEKQIPELNDEFVLELELDGVKTKEEYEKHV
                     EAELLKGKEAEAKRAREKAIIQTICDNAKVDIPESLIDMRKSQMVRQVESQAKQYGLS
                     LEQFLSFQGLDLEQYKEVLTEPAKASVTEEVVLDAIVSKEKIKLTKKELEAEIKKLAE
                     HYQMEVEEFKSKIDLQELESYLAIDKAKALVFEKAIIK"
     gene            5912..8230
                     /gene="lon"
                     /locus_tag="PLQ22_02075"
     CDS             5912..8230
                     /gene="lon"
                     /locus_tag="PLQ22_02075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOH58781.1"
                     /translation="MYKRDVNATGTLPTIVTNSLALIPDNEVFIDIAKSKSLESLRKA
                     ESKDKYLILLVQKDQEAKEIKNASDCYGTGILARAVYVQDKSSYRKVKFELIVRVSVE
                     EFNLETFETTFKTKPANVISLEEEAIYKKLLVAEVGDPLKAGEMFSNAHEITRIVKSD
                     ISVDEFINVLAYNLKVDNEKKHKYLETADIQKRFYFILDDIQGIKYINQLEQEINSKI
                     QKSISESQKEFYLREKMRAIQDELGDKAIREQDVENLREKILAAKMPQATEEKALEEL
                     KRYSTINPSSPESTVSKTYLDFLVDLPWYKKTEEVSDINKAQEQLDKDHYGLEKVKER
                     ILEYIAVHIQTKKNPLSILCLVGPPGVGKTSLASSIAKALNKKFVKQSLGGIRDVAEI
                     IGHRRTYVGALPGRILQGMKKAGVINPVFLLDEIDKLSSDYRGDPASAMLEVLDPEQN
                     KYFSDNYLEEPYDLSHVFFILTANYLEDIPYALRDRLEIVELSSYTEYEKFEIAKRHL
                     IPKQLELNGLDKDFILEDDALWEIIRKYTREAGVRELERMISSLIRKVIKKMLLEKAK
                     DIKVKKEDIETYLGKPIFLYNQVDLQDQVGVVTGLAYTHYGGDTLSVEATYFKGQGNL
                     QLTGQLGDVMKESAQAALSYVKANAAKFNIDPKIFKDIDIHIHVPEGAVPKDGPSAGV
                     TIATALVSLFSNKKVKHDVGMTGEVTLRGNVLPIGGLKEKSIAAARSGLKTILIPKEN
                     ERDITDIPELIQKDLSIILINSIEDAVGHALE"
     gene            8312..8548
                     /gene="secG"
                     /locus_tag="PLQ22_02080"
     CDS             8312..8548
                     /gene="secG"
                     /locus_tag="PLQ22_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOH58782.1"
                     /translation="MNAQVVDILFLIVSILLIVIIILQKSNEDASQAFTGKKSELFAN
                     KKERGIDVWINVITSVLSVAFFILAILAAFFVKR"
     gene            8603..>9184
                     /locus_tag="PLQ22_02085"
     CDS             8603..>9184
                     /locus_tag="PLQ22_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58783.1"
                     /translation="MEERILKLINNKKYQPLSVEELANVLNLDFKETMLAVDDLISKY
                     EVFISKKNKILNKDQANRYLGTISLTKNGLGFITYKEEIEDLFVGRDDLNSAMDGDLV
                     LFKADPFFEKAEVIEVIKRNIEYLVGEVVKDKRTYLLDTEDYHNLVLLKQDARLVVGD
                     VIKIKIVEYHDDYLVGEFFERIGKHNDFDIDILS"
BASE COUNT         3480 a         1006 c         1663 g         3035 t
ORIGIN      
        1 accaatgatg gatcgtatga aagaatcagt ttttaataca atagattctt atattgaagg
       61 agcaaatgtt ttagacttat ttggaggttc tggtgcttta tcattagaag ctatttccag
      121 aggtgctaag cactcatcta ttagtgagat taactatgaa gcttataaag tcattttaga
      181 aaatgtccaa actctaaaag aagaaaatag tgtaactatt tataaaatga gttatcaaaa
      241 gttattacaa gtcataaaag aaaatgagtt taagtttgat ttagtttttg tagatccacc
      301 atataagtta gaagttgcaa attcaattat tgaagaatta attcaaagtg gtaatcttaa
      361 tgacgaagca attattgttt cacattacta taaaggtact tatccagttt taactcaaga
      421 aaatcttgaa gttttaaaac ataatgttat gggtacaagt gaatatataa tttataagta
      481 tataaactaa taagaaaacc ctgtttagat agacagggtt ttgcttggag ttgtataatt
      541 ttctctttac aaattgtttg aaatagagta taatatagta tatgtcctgg tagctcagca
      601 ggatagagca acggccttct aagccgtcgg tcgggagttc gaatctcttc caggacgcca
      661 tttgtttatt tagtgctact attaataagt agcattttta ttaatcaaaa taaaaagcta
      721 gttttattaa ctagcttttt ttgcagcatt tggtagattt acaacaactt taccataatt
      781 agaaaatttc caaatggcag taacagcacc actaccatca tctaaggtag ttttaccttg
      841 acctgttaca ttattatttt caattgttag atagaagtaa gtatattttt ctgggttggt
      901 aaatgttgct ttcagcatat attgattttc tttaatatat tgtgaataat tatttatatt
      961 agtccattct ttaggtaatg ttccaggaag acgatcttca ttagaaattt gtttagtcca
     1021 cgtttgtttt acttcatcat atgtatagct gaaaatactt ctattttctt tcgcaacata
     1081 agtaatttgg ctaaaagttt ttaagccttc actatcataa acatcataag caattttagc
     1141 cttatcaaca tcaagttcaa cagcataagt tattttgctt tcatcttgac gaataaaaga
     1201 agttaccagt tgatagtttt ctttttcaag taatgtgaag aaatcttcta aagaattaat
     1261 cttttcctta caagatgtta aaagcggcaa taataatatt aatataaata gtgtaatagt
     1321 ttttctcatc tttttgctcc ttaataatta tattatacta tattgttctg cttttttcaa
     1381 tatcttatct ttaagctaaa atagtttaaa agtaaattag atcgttatta ttaagatatt
     1441 gctttttaga ggtggatatt atataataag aatagatagg agtaattata atatttagaa
     1501 aaattagtaa aataatttta ttattagttg tttttagttt tacattcact ttaatggctt
     1561 gtaaagataa aggggctgtt gctcatgaca ctcatattgt aggtgaagat tggatagtat
     1621 caagtgaaga acattggcat ggttgtacta ttgctggttg taaggaagta tttgataaag
     1681 gcaaacatgt ttatgaagaa ggtagcaatg tttgttctat atgtggttat aagaaaggaa
     1741 ctactccatc taaaccagat tctgacccag accaagatcc aaacacagat ccggatccaa
     1801 acacagatcc agatccaaac acagatccgg atccaaacac agatccagat ccaaacacag
     1861 atccagatcc aaatgcgagc gtgttagatt tattaagctc taattttaaa atgactatta
     1921 atactgtaag tgagtatgaa aatactgttg gtgaacttta tcgtgttggt aatgatttta
     1981 tgactgttta caaaatatat attgaaggct atggaacaca aataacaatt gattattaca
     2041 aatactatgc agagactgaa acatggacac gttatggtgc aacttctggt gatacaaaat
     2101 ggagtgaaaa ggtaacaaat ctttcagaag atgaagtcct ttatgattcg aatttagatt
     2161 taagattcat aattaattat ccagaaaaac cagataattt aggatatgtt gtaaataaag
     2221 gtgataaaga agacctttat ataagtgata agaaatacga atgtactatt tatacaacac
     2281 cagataatga atgccaatat catatttgtg ttatttctgg tttacaacac actttaaaac
     2341 tcaagttata tggagcaatg ataactatta cagaatataa aatgggagga gtaagtttcc
     2401 ctacatttcc aggggaaata cctaaccttc catagaagga gaagaaaatg aaaaaaagag
     2461 ttagtttact attattagtt tttgtaagtt tacttttaac tgggtgtggt agtagtacgc
     2521 ttaacattaa agatattgtt gagccacagt ttgttgattt tatttctgaa aaaaaagagt
     2581 actccaaacc acttacaatt gaaacaaatg aagaactaga taataattta tctagtaaaa
     2641 caattattgg ttttaaaaac aaaacatttt acaatctaga aactaaaaaa tttattgttt
     2701 ttgatcgtat gcctgctggt gaaattgaat atcgagtaag agatcaatat attgttatga
     2761 gtactgaaca atttcattat attcataatg gtaaaacagg aaattattta ttttctcttg
     2821 aaaaaggaga tgatgtaaca gtatttttct ttgcctctac tatttattat gattataaag
     2881 atgaacaagg aaacgataaa agtgataaat atgtttttac tgattataaa tataaaaaag
     2941 aagagattgt agaagaaggt gatgaggctc ctaattatga tgagataaat ggagactatg
     3001 gttatatcaa aattgatgat actaagataa aaatttttaa agatgatcgt acttatcatg
     3061 taattagctt taatgatggt tatgaaattg gttatgaaat tgatgattgg gcttggggtg
     3121 atcgaattga tggttggtat gttttaaaaa acggaaatat tctaatccaa agattcaaac
     3181 aagttgccaa cgatttccca acattccatt ttgtagatga cggtgttcgc tttgtaatga
     3241 aatcagaaat atttattgtt aagtcagcta ctttaaaaag tattgattta gattttgttg
     3301 ttgaagatgt aaataatggt ggtttttata aagctgataa cttagttcac ctttggcgaa
     3361 ttgattctat tttaaaagat tttacaggcg aagaatatgt tttctttgat aacgagataa
     3421 agtcagaaaa aagattagag tttaaatatg gtaatataga tgttactaat ttagagattg
     3481 ttggggagaa tcttttcatt tataatgacg agacgacaat ttatgcttta gatggtaata
     3541 ataaagttaa aggtgaaaaa cattatgatg ctgatgatta ttggttcgat ccattgctaa
     3601 atgggttgat tgaatttgaa gataagacta cttctaaatg tagtttatat aatattttag
     3661 acaataaagt gttgtatgaa gacctaacaa aattagttgg tgccaaacaa tatgcacttt
     3721 atagaaaaga tgatggtaaa attattatgg catctttata tggcttagtt gagatggaat
     3781 acactgacta tcgtacttat ggaggatcat gttataagtt gtatactgat gatgaaaaaa
     3841 cctttgaact atttaatatt caaggtaaat cgttaggaaa atttgaagat actacaattc
     3901 aagttatttc tagggatatt ggttttggcg gtgaagggta tcttgctatt agagttgaaa
     3961 aaggtttaga atctaaggtt ttagtttata aagtaaaata gaataaaaaa gaatctcttt
     4021 attaagagat tcttttttaa aagataaatt taaggtttta tgatataata taaaaagagg
     4081 tgggtatgaa taaatattat gaattgctaa aagaaaaaga atatcaaaaa atcaaagaag
     4141 aagcaattat taaaaatgat ttggatttgg attatttaat tgttttaatt agtgcttctt
     4201 ttaagacaaa tgaatttgtg attttgtatt actacctatc aaaaactaac tttaaaaagg
     4261 aaattgaaga tggtggagct aattatacta actatttaaa tattattgat gagaaacaac
     4321 aattagcatt agttcttaca aactttagtt taggagtaat taaagaaaaa aaagaacaag
     4381 agatagatga tgactattta aattatagag ttattgattt attaaatatt ttatttgaaa
     4441 ttggttatga agatgaagtt ttgaataaaa tttatcattt attaatgtct gtagaataga
     4501 gacttattac ttgcaaaatg agacatttta gagtataata ttaataatat tggagaagga
     4561 gtatatatat gaaaatagca gttaaaaaat tagagggtag tcaagcagag gtaaaagtta
     4621 ctttaaacaa agaagaattt agtaaatatt atgatgaagc ttttgataaa gtattagcag
     4681 actaccaagt ggaaggattt agaaaaggaa aagttccacg caatatttat ttaaatagag
     4741 taggctcaga tgctttagtt ttagaagagg cagttaattt aagtattaat ggaacttatt
     4801 ttgaggcaat tcaagaaaag aaagttagag ctgtaaatta tccaaaagtt gactttgatc
     4861 ctaatcaagt taaaaaagat ggtggattca cttatacagc tgttgtagat gtatatcctg
     4921 aggtagaatt aggagagtac tttggaattg aatataaaga ggaaaagcaa gaaatatcta
     4981 aagaagaact agaaagagtt ttaagacaag aaagagaaag aaaagccgtt ttagttttaa
     5041 aagaagatgg ttgtattgaa ttaggaaata ctgttgtttt tgattttgaa ggatttgtag
     5101 atggagtagc atttgaaggc ggtaaagctg aaaattattc tttagaaatt ggctctggtc
     5161 aatttattcc tggatttgaa gaacaaatgt taggaatgaa agaaggagaa gaaagagagc
     5221 taaatgttaa gtttccagac gaatatcatt cagaaaattt aaaaggaaaa gatgctatct
     5281 ttaaagtaaa agttcatgaa attaaagaaa aacaaattcc tgaacttaac gatgagtttg
     5341 ttttagaact tgagcttgat ggtgtaaaaa ctaaagaaga atatgaaaaa catgtagagg
     5401 cagaactact aaaaggaaag gaagctgaag ctaaacgagc tcgtgaaaaa gcgattatcc
     5461 aaactatttg cgataatgcg aaagttgata ttcctgaaag tttaattgat atgagaaaga
     5521 gtcaaatggt tagacaagtt gagtctcaag caaaacaata tggtttatct ttagagcaat
     5581 tcttatcttt ccaaggttta gatttagaac aatataaaga ggttctaact gaaccagcta
     5641 aagcatcagt tactgaggaa gtagttttag atgctattgt ttcaaaagaa aaaattaaac
     5701 taacaaagaa agaattagaa gcggaaatca aaaagctagc agaacattac caaatggagg
     5761 tagaagagtt taaatcaaag attgacttac aagaactaga gtcatactta gctattgata
     5821 aagctaaagc actagttttt gaaaaagcaa ttattaaata aataagggct ttagcccttt
     5881 taaaatctaa tcaaaaagtg aggtgaaaga aatgtataaa agagatgtaa atgctactgg
     5941 tactttacca acaattgtaa ctaacagctt agcgcttatt cctgataatg aagtatttat
     6001 cgatatagcg aaaagcaaat cactagagtc tttgagaaag gcggaaagca aagacaaata
     6061 tttaatttta ttagtgcaaa aagatcaaga agctaaagaa attaaaaatg caagtgattg
     6121 ttatggtact ggtattttag cacgtgcagt ttatgttcaa gataagtcta gttatcgtaa
     6181 agttaagttt gaacttattg ttagggttag tgttgaagag tttaatttag aaacgtttga
     6241 aacaacattc aagactaaac cagctaatgt tatttctcta gaagaagaag ctatttataa
     6301 aaagctatta gtagctgaag taggagatcc acttaaagca ggagaaatgt ttagtaatgc
     6361 tcatgaaatt acaaggattg taaaatcaga tatttcagta gatgagttta ttaacgttct
     6421 tgcgtataac ttaaaagtag ataacgaaaa gaaacataag tatttagaaa ctgctgacat
     6481 tcaaaagaga ttttatttta tcttagatga tattcaaggt attaaataca ttaatcaatt
     6541 agaacaagaa attaattcaa agattcaaaa gtcgattagt gaaagccaaa aagagtttta
     6601 cttaagagaa aaaatgcgtg ctattcaaga tgaattaggt gataaagcta ttagagagca
     6661 agatgtagag aacttaaggg aaaagattct tgcagctaag atgcctcaag caactgaaga
     6721 aaaagcgcta gaagaactta agcgttatag tacgattaac ccatctagtc ctgaatctac
     6781 agtaagtaaa acttatcttg atttcttagt tgatcttcct tggtataaaa aaacagagga
     6841 agtttcagat attaataaag ctcaagaaca actagataaa gaccattatg gtttagaaaa
     6901 agttaaagaa agaattttag agtatattgc cgttcatatt caaacaaaga aaaacccact
     6961 atcaattctt tgtttagtag gccctccggg tgttggtaaa acatcacttg catcttcgat
     7021 tgcgaaagca ttaaacaaaa agtttgtaaa gcaatcatta ggtggtatta gagatgtagc
     7081 tgagataatt ggtcatagaa gaacatatgt tggggcctta cctggtagaa tcttacaagg
     7141 tatgaaaaaa gcaggagtta ttaatcctgt attcttactt gatgagattg ataaattatc
     7201 aagtgattat aggggagatc ctgcaagtgc aatgttagag gttttagacc cagaacaaaa
     7261 caagtatttc tctgataact atttagaaga accatatgac ttatcacatg tattctttat
     7321 tttaactgct aactatttag aagatatccc ttatgcttta agggatagat tagagattgt
     7381 tgagctttct tcttatacgg aatatgaaaa atttgaaatt gctaagcgtc atttaattcc
     7441 aaaacaatta gaactaaatg gtttagataa agattttata cttgaagatg atgctttatg
     7501 ggagataatt agaaagtata caagagaagc aggagttaga gagttagaaa gaatgatttc
     7561 ttctctaatt agaaaagtta ttaagaaaat gcttttagaa aaggctaaag atattaaagt
     7621 taagaaagaa gatattgaga cttatttagg aaaaccaatc ttcttatata accaagtaga
     7681 tttacaagac caagtaggtg ttgtaactgg acttgcttat actcattatg gtggagatac
     7741 tttatcggtt gaagctacat acttcaaagg acaaggcaac ttacaattaa caggacaact
     7801 tggagatgta atgaaagagt cagctcaagc tgctttaagt tatgtaaagg ctaatgcagc
     7861 taagtttaat atcgatccaa agatctttaa agatattgac attcatatcc acgttcctga
     7921 aggtgcagtt ccaaaagatg gaccatcagc aggggttacg attgctacag ctttagtatc
     7981 attattttca aataagaaag taaaacacga tgtgggtatg actggtgaag ttactttaag
     8041 aggtaacgta ttacctattg gtggtttaaa agaaaagtca attgcagctg caagatcagg
     8101 attaaagacg attttaattc caaaagaaaa cgaaagagat attacagata ttccagagtt
     8161 aattcaaaag gacttatcaa ttattttaat caatagtatt gaagatgcag ttggtcatgc
     8221 tttagaataa aaagggattt aatccctttt tatttttatt gctttttaat agtaaatatg
     8281 ctataataca atataagttg tggaggatat tatgaacgca caagttgtag atattttatt
     8341 tttgatcgta tcgatcttat taatagttat tattatttta caaaaatcaa atgaagatgc
     8401 ttcgcaagca tttacaggta agaagtcaga attgtttgcg aataagaaag aaagaggaat
     8461 agatgtttgg attaacgtaa ttacatcagt attatcagtt gctttcttta ttttagcaat
     8521 cttagcagct ttttttgtta aaagataggt aactaaattt aaggtcttgg tataagacct
     8581 ttattttttg taaggagaga taatggaaga aagaatatta aaattaatta acaataaaaa
     8641 atatcaacca ctaagtgtcg aagagttagc aaatgttctt aatttagatt ttaaagaaac
     8701 gatgcttgca gttgatgatt taataagtaa atatgaagtc tttattagta agaaaaacaa
     8761 aattttaaat aaagaccaag ctaatcgtta tttaggtact atctcactta caaaaaacgg
     8821 tttaggtttt atcacctata aagaggaaat agaagatttg tttgtaggcc gagatgactt
     8881 aaatagcgca atggatggag atttagtgtt atttaaagct gatccttttt ttgagaaagc
     8941 tgaagttatt gaagttatca aaagaaatat tgagtactta gtaggggaag ttgttaagga
     9001 taaaagaact tatcttttag atactgaaga ttatcacaat ttagttttat taaaacaaga
     9061 tgcaagattg gttgtaggcg atgtaattaa aattaagatt gtagaatatc atgatgatta
     9121 tttagttgga gagttttttg aaagaattgg aaaacacaat gattttgata ttgatatttt
     9181 aagt
//
LOCUS       DAOSGS010000029         8994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6597_length_8994_cov_3.810605, whole genome shotgun sequence.
ACCESSION   DAOSGS010000029 DAOSGS010000000
VERSION     DAOSGS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_8994_cov_3.810605"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLQ22_02090"
     CDS             complement(<1..408)
                     /locus_tag="PLQ22_02090"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOH58784.1"
                     /translation="MKLMILNGPNLNFLGIREKEIYGNKTYQDLEKYLYEYSKTKNIE
                     LLIKQTNYEGELIDYIQKGYLEQYDGIIINPGALTHYSYSLYDAILSVKPLKVIEVHL
                     TDPKLREDFRKKSVISEVCFKTISGLGFESYKLA"
     gene            complement(411..1178)
                     /locus_tag="PLQ22_02095"
     CDS             complement(411..1178)
                     /locus_tag="PLQ22_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOH58785.1"
                     /translation="MSKFSTTNPVFRKFRDTKDDLGVVETNTASYKGIAKKVLFFFLF
                     VLVGAGVGVGLLNTIFAGQEEFGAPYIAILAIAVIGTLVCSIIASFVPKATKVAGTLY
                     CLFEGFLVGSISYIFGSITEGAVPIALIGTLSVFLVVTALYVTGLVKVTNKFVNFLII
                     LTVSFIVGSLAMWIFVLITGFTIDLGLVILIGSLSLLISIMYLFVDIKNIHMVVENQF
                     PKESEWYAAFGLTFTLIWIYIEILRLAFIILSRVKRK"
     gene            complement(1231..5775)
                     /locus_tag="PLQ22_02100"
     CDS             complement(1231..5775)
                     /locus_tag="PLQ22_02100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOH58786.1"
                     /translation="MNNNGFLKILEKLNLKTSDDIREGKLESIEVNDLTKKAVFKYSF
                     PKVPNVHFLEEFISKSETGLKEIFNLNEIESVVLYPNNEIEETTLEEYYKHILENLTS
                     KKPLYKVLENFNTNFSDNKVVFYVASKEDEATLGNAFKEIEAIFKSYFLDFVSVEAVI
                     SHFEKPMEDDIKYNITKIYTEYDNQPKTQPTGIVKKTNKDYRSKTRKKTLEVNIAPSK
                     IKDLPVDEMDLVGFRQLNFGDAKVAVEGEIIKSEIINRGIHNFFEAVIQDDTGVILLK
                     SYVKDENRRLFEEDLVKGKIVKVIGYLDYDKFSRCITLKFVNYVPLGVVTKDHLKDNS
                     DVKRVELHAHTKMSAQDGVLDIKDYVKRASLFGHQAIAVTDTNSLQALPDLDSYCEEY
                     NIKPIYGLESYLIKESDYLITPSKLKIDRNLKDARFVVYDLESTGLSSNYNEIIEISA
                     VRLYKGEIVGEFNSFVKPKAKISNFITKLTSITNDDVRNAPTIEQVLPEFIDFIGDDI
                     LVAHNSSFDNTMLYANMKRIGIDNITFPTIDTLKLAHNFYNHRLARFNLKALAKEFNV
                     DLIQHHRAINDARATAQIFLKMLNDLEQMGIKTYNSLNDSIIEDAYKYVFPYHLTLLA
                     TNRKGIKHLNEIVSLSLTEHFYKEPRLLEKVLDEKREGLLVGSGCQNGEIFTIAYNGS
                     IEELEERMEYYDYIEIQPPYQYAYLFSKNYDKQDIDNEGSSQDIKEALELISTEYEEQ
                     IKDTIKKIITVARKMGKIVVATGDVHQLDEEDTILRQIFVEAPQVGGGIHELAKAGIV
                     LPQHYMTTEEMLSHFLFLGEATAYEIVVTNTNLINDKIEQLSLFPKKLFAPKDDFLVD
                     QGIQSVEEELKRITYQKAESLYGNPLPKYVNERLKKELKNIIDNDYATIYYISYMLTN
                     KCKEDNSVVGSRGSVGSSFVAYCMNITDVNCLVPHYYCPDCHYNAFKYSIAEQGKYED
                     KIPDYLLENLSLVTNGLDLKSAKCPKCGKDLVGDGCDIPFETFLGFKGDKVPDIDLNF
                     SSEYQTTAHDYTRELFGENNVFRAGTVSTVQGRTAYGYVKNFLDRHDKKMSKTEMDII
                     IEKITGVKRTTGQHPGGIVVIPKNIDFSDVVAIQYPSDNVDSDWKTIHYEYDKYEKNL
                     LKLDLLGHRDPTMISKLMEYVERYPEEFPFKEYEDIPLNDPKVLSIFNSVSALGVTAE
                     EVLEQIGTTGIPEFNTPLGKEMLSDIKPKTVSELIKISGFGHGTNVWSGNQRELYLGN
                     TLNNKVDFDLLIGCRDDIMLYLVNKGVDSLTAFKIMESVRKGRSLTTKQEQVLQEHNV
                     PEWYIDSCKKIAYMFPKAHAAAYVMMALRIAWFKVYKPLYYYAVYFEVRGEAFDIEAM
                     PLGYEAIREALKAANLEKEENQKRRKIDVKLDALIDTLSLALEATARGFKFKQVDLDK
                     SEASALTIEGNSLYLPFSALPSVGATTALSITEARSERRFSSVSDLRQRTKISKTIID
                     HMRKLGILDHLPKDDQINLF"
     gene            complement(5898..7949)
                     /locus_tag="PLQ22_02105"
     CDS             complement(5898..7949)
                     /locus_tag="PLQ22_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOH58787.1"
                     /translation="MRLDEKIRNSKNAIPEMENDLRKQIYSASIEPKKTSRFKFRFAY
                     MSFVALLLLGLGIFIGLSVNTLRPSQYSNDSQTFRTDMEVQDFLNQEPKASGMDISLE
                     DLFRFSGFSYSEAKDGSPETNGPTSTDETNPGGTSGANVQVKGVDEADYLKYDANKLY
                     YVHPNVMIDGKSRTAFEIWKLNEGFTGLLGYYLVDEGYAFTALVANNTHVVVVNYNTG
                     YYGYYRYYEAENKYLDNIEILIFEKEKIKIDADGILEPNKRIIMPGFLTLSSDQKYLR
                     LTDKALLINSTSYIRDGNVPQLSYEFDDRKEEITKDQISYYKDSYNRLFNIFMKVSLE
                     DDYKINTYHWFGSSPTVYVSHTAFYYTERTYNYTNTTGADYTSKTRITKLDFSGDEIV
                     VKGNVLVDGIVRNQWYMDEFEGRLRIVAADPNISGWWSDREKPTKHAVYVVQEKELEK
                     GLEIVGSLTEGIGLPGEDIKSVRFNTNAETGLNVLNIVTYRNTDPLYKILFRDEIKPY
                     IEAELKVPGFSFYLHPITIEEKQLLLGIGIGEEWSDKISLYNNDDELTQIGKDFLINE
                     EVARYLGSDDIYAWNSTRSHLEYIDVFGFSEGHLFGMPIEVYSQETSEEEVKWAYYKS
                     YMMLCINPDATSPIEIWKVFEGDKIVASGYYNLNDYHNYRVIFANGYFYGVGQNVIGL
                     N"
     gene            complement(7936..8424)
                     /locus_tag="PLQ22_02110"
     CDS             complement(7936..8424)
                     /locus_tag="PLQ22_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH58788.1"
                     /translation="MKRLSEEQFYEKYELYAKMIYNIAYSHLKNVNHSEDVVQEVFLK
                     YLNNYHKLESLDHEKFWLIRVTMNEAKNVLKSSWNTKVNVIEEDSYPYQDEVNEEKRF
                     FEMIYNLPDKYKIVIILFYYENLDIKEISKVLSLSEAAVKKRLERARNILKEEESKNE
                     VR"
     gene            complement(8577..8653)
                     /locus_tag="PLQ22_02115"
     tRNA            complement(8577..8653)
                     /locus_tag="PLQ22_02115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8617..8619),aa:Glu,seq:ctc)
     gene            complement(8681..>8994)
                     /locus_tag="PLQ22_02120"
     CDS             complement(8681..>8994)
                     /locus_tag="PLQ22_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HOH58789.1"
                     /translation="PVLLIYDGATIGHKALRELIIQTAEEEKIPYQVDHLTRGGTDAG
                     ALHTSHHGSPAISFCIATRYIHSHSGVIHYDDYENGVKLLTAVIKKLDRKTVDKLKYG
                     E"
BASE COUNT         3070 a         1616 c         1010 g         3298 t
ORIGIN      
        1 tgctaattta taagattcaa atcctaaacc agatattgtt ttaaaacaaa cttcactaat
       61 tacacttttc ttacgaaaat cttctcttaa tttaggatca gttaaatgaa cctcaattac
      121 ctttaaaggt tttactgaca gaattgcatc atacaaacta tatgaataat gagttaaagc
      181 tcctggatta atgataattc catcatactg ttctaaatat cctttttgga tataatcaat
      241 caattcacct tcataattag tttgtttaat aagaagttct atatttttag ttttagaata
      301 ttcatataaa tacttttcta aatcttgata agttttattg ccataaattt ctttttctct
      361 aattcctaaa aaattaagat taggtccatt taatatcatt aacttcatag ctattttctt
      421 ttaactcgac tcaaaatgat aaaagcaagt cttaaaatct caatgtaaat ccaaattaat
      481 gtaaatgtaa gaccaaatgc tgcataccat tctgattcct tcggaaattg attttctaca
      541 accatatgaa tattctttat atcaacaaac aaatacataa tactaataag caatgaaaga
      601 cttccaatta aaattactaa tcctaaatca attgtaaatc ctgtaattaa aacaaaaatc
      661 cacattgcta aactaccaac tatgaaacta actgtaagaa taatcaaaaa gtttacaaac
      721 ttatttgtta ccttaactag tccagtcaca tacaaagcag ttacaactaa gaaaacagaa
      781 agtgtgccaa ttaaagctat cggtactgct ccttcagtaa tagaaccaaa aatgtaagat
      841 atagacccta ctaaaaaacc ttcaaataaa cagtataaag tacctgctac tttggtagct
      901 ttaggaacaa agctagcaat aatgctacaa acaagtgtac caataactgc aatagctaaa
      961 atcgcaatat aaggagcacc aaattcttct tgcccagcaa atattgtatt taataatcca
     1021 acaccaactc ctgctcctac taatacaaat aggaagaaaa ataatacctt tttcgctatt
     1081 cctttataac ttgcggtatt tgtttctaca acacccaagt catcttttgt atctctaaac
     1141 ttacgaaata caggatttgt tgtactaaat ttcgacatat ttctcacctc tcttcattat
     1201 atattataat ttataatatt taaataaatc ttaaaataga tttatctgat catctttagg
     1261 taaatgatct aaaataccta actttctcat atgatcaata attgtttttg aaatctttgt
     1321 tctttgcctt aaatcagaaa cagaactaaa tcttctttca cttcttgctt ctgtgataga
     1381 taaagcagtt gtagccccta cagaaggtaa agcactaaat ggtaaatata aactattacc
     1441 ctcgatagtt aatgcagaag cttcagattt atctaaatct acttgtttga atttaaaccc
     1501 acgagcagtt gcttccaaag ctaaactcaa agtatcgatt aaagcatcta gttttacatc
     1561 aatctttctg cgcttttgat tttcttcctt ttcaagattt gcagctttta acgcttccct
     1621 aatagcctca tatcccaacg gcatagcttc aatatcaaaa gcttcccctc ttacctcaaa
     1681 ataaacagca tagtaatata aaggtttata aactttaaac caagcaattc ttaaagccat
     1741 cattacataa gctgccgcat gagctttagg aaacatataa gcaattttct tacaagaatc
     1801 aatataccat tcaggaacat tatgttcttg caatacttgt tcttgtttag ttgttaaact
     1861 acgtcctttt ctaactgatt ccatgatttt aaatgctgtt aaactatcaa cacctttatt
     1921 tactaaataa agcataatgt catcacgaca accaattaac aaatcaaaat caactttatt
     1981 atttaaagta tttcctaaat ataactctct ttgatttcca ctccaaacgt tagtaccatg
     2041 accaaatcca gagattttaa ttaattcaga aacagtctta ggcttaatat cactaagcat
     2101 ttcttttcct aatggtgtgt taaattcagg aattcctgta gttcctattt gttctaaaac
     2161 ttcttctgcc gtaactccca aagcagaaac tgagttgaaa atacttaaaa ctttaggatc
     2221 gtttagtgga atatcctcat attctttgaa tggaaattct tccggatacc tttctacata
     2281 ctccattaac ttactaatca ttgttggatc acgatgaccc aaaagatcta gtttaagtaa
     2341 attcttttcg tatttatcat attcataatg aatagttttc caatctgaat caacattatc
     2401 agatggatat tgaatcgcaa caacatcaga aaaatctata ttcttaggaa taacaacaat
     2461 acctcctgga tgttgtcctg ttgttctttt aacgcctgta atcttttcaa taataatatc
     2521 catctcagtc tttgacattt tcttatcatg acgatccaaa aagttcttaa cataaccata
     2581 tgctgttctt ccttgaaccg tacttacagt tccagcacga aaaacattat tttctccaaa
     2641 taattctcta gtataatcat gtgctgttgt ttgatattca cttgaaaagt ttaaatcaat
     2701 atcaggaact ttatctcctt taaaacctaa aaaagtttca aaaggaatat cacaaccatc
     2761 acctactaaa tctttgccac acttagggca ttttgctgat tttaaatcta aaccattagt
     2821 aactaaactt aaattttcaa gtaaatagtc aggaatctta tcttcatatt taccttgttc
     2881 agctatagaa tatttaaaag cattgtaatg acaatcaggg caataatagt gtggtactaa
     2941 acagttaaca tcggtaatat tcatacaata tgctacaaaa gatgaaccta ctgaacccct
     3001 actaccaaca actgaattat cttctttaca cttatttgta agcatataag aaatataata
     3061 gatagtcgca taatcattat ctataatatt ttttagttcc ttctttaaac gttcatttac
     3121 atatttaggt aaagggttac catataaact ttcagctttt tgataagtaa ttctctttaa
     3181 ctcttcttca actgattgaa taccttgatc aactaagaaa tcatctttag gtgcaaataa
     3241 ctttttaggg aaaagagaca attgttcaat cttatcatta attaaattag tattagttac
     3301 tactatctca taagctgttg cttcacccaa aaataggaaa tggctcaaca tctcttctgt
     3361 agtcatatag tgttgaggta aaacaatacc agcttttgct aattcatgaa taccaccacc
     3421 aacttgtggt gcctctacaa atatctgtct taaaattgta tcttcttcat ctaattgatg
     3481 aacatcacca gtagctacaa caatcttacc cattttacga gcaacagtta taatcttttt
     3541 aattgtatct tttatttgtt cttcatactc agtactaatt aattctaaag cttcctttat
     3601 atcttgactg ctaccttcat tatcaatatc ctgtttatca tagtttttac taaataaata
     3661 agcatattga tatggtggtt gaatttcaat ataatcataa tattccattc tttcttctaa
     3721 ctcttcaata gaaccattat aagcgattgt aaaaatttca ccattttggc acccactacc
     3781 aactaataat ccttcacgtt tttcatctaa aactttttct aaaagtcttg gttctttata
     3841 aaagtgttca gttaaagata aagaaacaat ttcatttaaa tgcttaattc ctttacggtt
     3901 tgtagcaaga agggttaagt gataaggaaa tacatattta taagcatctt caataatgct
     3961 atcatttaaa ctattgtatg tcttaatccc catttgctct aaatcattta acatctttaa
     4021 aaagatttgg gctgtagcac gagcatcatt aatcgctcta tggtgttgaa ttaaatctac
     4081 attaaactct ttagctagtg ccttcaaatt aaatctcgct aaacgatgat tatagaaatt
     4141 atgagcaagt tttaaagtat cgattgttgg aaaagtaata ttgtcaattc caattctctt
     4201 catgtttgca taaagcatcg tattatcaaa acttgaatta tgagcaacta aaatatcatc
     4261 accaataaaa tctataaatt caggtaaaac ttgttcaata gttggagcat tccttacatc
     4321 atcattagta atactagtta atttagtaat aaaattacta atcttagcct taggctttac
     4381 aaaactatta aactcaccaa caatctctcc tttataaagt ctaacagcag atatttcaat
     4441 aatttcatta tagttactag aaagaccagt tgactctaag tcataaacta caaaccttgc
     4501 atcttttaaa ttgcgatcta tttttaattt agaaggcgta attaaataat cactttcttt
     4561 aattaaatat gactctaaac cataaatagg tttaatatta tattcttcac aataactatc
     4621 taaatccggt aaagcttgca aactattagt atcagtaaca gctattgctt gatgaccaaa
     4681 taaactagct ctcttgacat aatctttaat atctaaaact ccatcttggg cactcatttt
     4741 cgtatgagca tgaagttcta ctcttttaac atcgctatta tcttttaaat gatctttagt
     4801 cacaactcct aaaggaacat agtttacaaa ctttaaagta atacaacgac taaatttatc
     4861 ataatctaaa taaccaatta cctttacaat cttacctttt actaaatcct cctcaaaaag
     4921 tcttctattt tcatccttca cataagattt aagtaatata actcccgtat catcttgaat
     4981 aacagcttca aagaaattat gaattcctct gttaataatc tcagatttaa taatttctcc
     5041 ttcaaccgca actttcgcat caccaaaatt aagttgtcta aatccaacta aatccatttc
     5101 atcaactggt aaatctttaa tttttgatgg agcgatatta acttctaaag ttttctttct
     5161 tgttttgcta cgataatctt tattagtttt ctttacaatt ccggttggct gagttttagg
     5221 ttgattgtca tattcagtat aaattttagt aatattatat ttgatatcat cttccattgg
     5281 cttttcaaaa tgagaaataa ctgcttctac agaaacaaaa tctaaaaaat agctcttaaa
     5341 aattgcttca atttctttaa aagcattacc taatgtagct tcatcttctt tactagccac
     5401 ataaaagact actttgttat cgctaaagtt agtattaaaa ttctctaaaa ccttatataa
     5461 aggtttttta gatgttaagt tttccaaaat atgtttatag tattcttcca gtgttgtttc
     5521 ttcaatttca ttatttggat ataaaacaac tgattctatt tcatttaagt taaaaatctc
     5581 ttttaatcct gtttcactct tagagataaa ctcctctaaa aaatggacat taggaacttt
     5641 aggaaaagag tatttaaaaa cagccttttt tgtaaggtca tttacttcaa tactttcaag
     5701 ttttccttct ctaatgtcat cacttgtttt tagatttaat ttttctaata ttttcaaaaa
     5761 accattgtta ttcataaaca acacctcatt atattttatc atattcaata acttttttct
     5821 attgttattt atataaaaaa ggatttcctt gctaagcaag aaaacccttt ttttggagaa
     5881 gttctaaaat ttactattta atttaatccg attacatttt ggccaacacc atagaaatag
     5941 ccgttagcaa aaattacacg gtaattatga taatcattta agttataata gccactagca
     6001 actatcttat ctccttcaaa tactttccag atttcgattg gagaagttgc atcaggatta
     6061 atgcatagca tcatatagct cttatagtat gcccatttta cttcttcttc actagtttct
     6121 tgactatata cttcaattgg cattccaaat aaatgtcctt cgctaaaacc aaatacatca
     6181 atatactcta aatgtgatct agtagaattc caagcataaa tatcatcgct acctaaatat
     6241 ctagctactt cttcattgat taagaaatct ttaccgattt gagttaattc atcatcgtta
     6301 ttatataaag atattttatc tgaccattct tcgccaatac caattccaag taaaagttgt
     6361 ttttcttcaa ttgtgattgg atgtaagtag aaactaaatc caggaacctt taattcagct
     6421 tcaatataag gtttaatttc atctctaaat aggatcttgt ataatggatc agtattacgg
     6481 tatgttacaa tatttagtac atttagacca gtttctgcgt tagtattaaa ccttactgac
     6541 ttaatatctt cacctggaag accaataccc tcagttaatg aaccaacaat ttctaatccc
     6601 ttttcaagtt ctttttcttg aactacataa actgcgtgtt ttgtaggttt ttctctatct
     6661 gaccaccaac cagaaatatt aggatcagct gctacaattc ttagtctgcc ttcaaattca
     6721 tccatgtacc attgatttct aacaatacca tcaactagta cattaccctt aacaacaatt
     6781 tcatcgccag aaaaatcaag tttagtaatt cttgtttttg aagtataatc tgctccagtt
     6841 gtattagtat aattataagt tctttcagta tagtaaaatg ctgtatggct tacatatact
     6901 gttgggctac taccaaacca atggtaagta ttaatcttat aatcatcctc taaactcact
     6961 ttcatgaaga tattaaataa acggttataa gaatctttat agtaagagat ttgatctttt
     7021 gtaatttctt cttttctatc atcaaattca taacttagtt gaggaacatt tccatcacga
     7081 atatatgatg ttgagttaat caataaagct ttatctgtaa gtcttaagta tttttgatct
     7141 gaagataatg ttaagaaacc tggcataata attctcttat ttggctctaa tataccatca
     7201 gcatcaatct taattttttc tttttcaaaa attaaaattt caatattatc taaatacttg
     7261 ttttctgctt catagtatct ataatagcca taataacctg tattatagtt tactacaaca
     7321 acatgtgtgt tattagctac taatgctgta aaagcataac cttcatcaac taagtaatag
     7381 cctaataatc ctgtaaagcc ctcatttaat ttccaaattt caaatgctgt tctagattta
     7441 ccatcaatca ttacatttgg atgtacataa taaagcttat tcgcatcata ttttagataa
     7501 tcagcttcgt ctaccccttt aacttgaacg tttgcacctg atgtcccacc tggattagtt
     7561 tcatcagttg aagttggtcc attagtttct ggagaaccat cttttgcttc tgaataacta
     7621 aatccactaa aacggaaaag atcctcaaga cttatatcca tcccacttgc ttttggttct
     7681 tgattcaaga aatcttgaac ttccatatca gttctgaatg tttgtgaatc atttgaatat
     7741 tgtgatggtc ttaaagtgtt aacacttaaa ccaataaata ttcctaggcc taataacaat
     7801 aaggctacaa aactcatata agcaaatcta aatttaaatc tacttgtttt ttttggttct
     7861 atacttgcac tataaatttg ttttcttaaa tcattttcca tttctggaat tgcatttttt
     7921 gaatttctaa ttttttcatc taacctcatt ttttgattcc tcctctttta agatgttacg
     7981 agcacgttct aatcttttct ttactgcagc ttctgaaaga cttagaactt ttgaaatttc
     8041 ttttatatct aaattttcat aatagaataa aataattact attttatatt tatcgggtaa
     8101 attgtaaatc atttcaaaaa atcttttttc ttcattaacc tcatcttgat acggataact
     8161 atcttcttca ataacattga ctttcgtatt ccatgaagat tttaaaacat ttttcgcttc
     8221 attcattgta actctaatta accaaaactt ctcatgatct aatgattcaa gtttgtggta
     8281 attgtttaaa tattttaaaa acacttcttg gactacatct tctgaatgat taacattttt
     8341 taagtgtgaa tatgcgatat tataaatcat cttcgcatat agttcatatt tttcataaaa
     8401 ttgttcttcg cttaatcttt tcatattaac ctctccgtat atactaatta tatcataaac
     8461 gaacctattt tatttaccct ttctacctat aatacaaata accaaggcaa aaagtgacaa
     8521 aaataatata ttaaaaacaa aaaagctttc tttaaaagaa agcttttaat atcaaatggt
     8581 gacctgtatg ggattcgaac ccatgaatgc aagcgtgaga ggcttgtgag ttaagccact
     8641 tctccaacag gccaagtaga ataaagcatt ttaaaatgct ttattcacca tatttaagtt
     8701 tatctacagt tttacgatct aactttttaa taactgcagt taaaagttta actccatttt
     8761 cataatcatc ataatggata actcctgaat gtgaatggat gtaacgagtt gcaatacaaa
     8821 atgaaatcgc tggacttcca tggtgagaag tatgtaatgc acctgcatca gttccgcctc
     8881 ttgttaagtg atcaacttga taaggaatct tttcttcttc agctgtttga ataattagtt
     8941 ctcttaaagc tttatgacca attgttgctc catcataaat caataatact gggc
//
LOCUS       DAOSGS010000030         8760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6834_length_8760_cov_5.975187, whole genome shotgun sequence.
ACCESSION   DAOSGS010000030 DAOSGS010000000
VERSION     DAOSGS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_8760_cov_5.975187"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLQ22_02125"
     CDS             complement(<1..495)
                     /locus_tag="PLQ22_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH58790.1"
                     /translation="MKFVVLASGSKGNCIYIETQNHKILVDVGIAYSNAKTRAEKLGI
                     DLSEVDAIFLTHSHIDHIYGLTQFLKQTKATVYASYDTHKYLDHKYKYYNSLGNIKVI
                     EADALYNILDFFVVPLQLVHDFPCFGYYFIHQEKTLMIATDTGIFPEKYTSLLEATDA
                     LIIEA"
     gene            complement(586..3291)
                     /locus_tag="PLQ22_02130"
     CDS             complement(586..3291)
                     /locus_tag="PLQ22_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58791.1"
                     /translation="MKRRRRVKSKANKQKDAPKLSPKTILLSTLPVLLVVLVIVVIAV
                     ASTVAKNNRKGPMIADPDGQYVLVGEGDNEYSLTKGEMYSLLKDQYGINTLFEMLDRE
                     LLENGEKNYLAEVTDEAVQEELEKEMYKGLDLDKAEAEEKAEAEEKYYNERYVQGLKT
                     EAEIKDYYRLELAKKAYAAKYLEDEIAKKDAEAEDDTEKYFPKSKIDTKYNALYKEQY
                     FVIVLEYATRKDAYGALEQLGYKIKDQEKDASGALIPDSYNKWMKIPNEEQAEEELTR
                     EETIKAFIDLYNTLHSYKVEGYPDETLTLKEGVQYTINEDGHYVFHQEVIKDEDGNVS
                     DDPKNALLYTDEELKAIGAAVLSDVMNFKAYDPETSVVNKDQRWFKNYFMSLDGGKKF
                     ATILKIKTIEKPALEDVQDKVIEALKEETLTAAYVTKAMYGLRATADLVLYDKEIETY
                     YKSQADTYKIEFETSKARSKDLIAKLGDIEYTVEDFYHKLNQDYGMNIAINEINFKRI
                     LNNPDINKIYDVEKEKVLDKEAWAKIETKFTDEKAQYANYSMYMTWTDYMKYRFQANN
                     EQEVKVRLLYEQLSAEASKPYTDFSLISETDPIWTKYYSKNMENVKSKYYKVEGYHLL
                     IHVVDEEKSTSSSQVLLDPKFWTPKQVELAEELYAQVQAYLVAKGDYKAQFDKIVTAF
                     NEAPLFQAHLPQDAASQPEFTINELEYDLDDMIDISKFKTAGLKVKYESLGSFEFKLP
                     GQSGGMVEAFNEGIKKIWDEDDDSAEDKIFGVNTVVGEHLITEFGYHVYVNSKTTPLE
                     TYTEGDVEHILPTFDMVKKYLAGEALDTDTTKAAAKKKIVEFWVKGIKEDAVKNNQHI
                     LTSKQKGLTFDFKEADFTNADYQKFLGIVLKEGQEALIYKAHLAD"
     gene            complement(3368..4309)
                     /locus_tag="PLQ22_02135"
     CDS             complement(3368..4309)
                     /locus_tag="PLQ22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH58792.1"
                     /translation="MRTKVKDYRAGDEVNNLILRLVNVQYRKTTSQADYVNALGFDGE
                     EILEVKIWNVTDSIRENLENGKIYTCSGQMKDYQGKLQFNLKELAEADPSLYRLEEFF
                     EYAKLSVEELKEKIMNYVLKIENANIKKLVLAVLKEDLDAYFVHPAAKSVHHNYISGL
                     AYHVYSMLKLADTYIELYPFLNKDLIYGGILLHDFGKITEMEKTTGEYTKQGNLLGHI
                     IIASNILYEKAKELSLEESEEYLMLAHIIIAHHGFLEFGSPKEPTIPEAYVVFLCDYA
                     DSRMGALEKELKDGVKGEYSQPVFAFNKRIFYLPDLD"
     gene            complement(4321..5541)
                     /locus_tag="PLQ22_02140"
     CDS             complement(4321..5541)
                     /locus_tag="PLQ22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58793.1"
                     /translation="MSTNLGYINKGKEILTVLNTNGYQAYFVGGVVRSIILDIPFSEI
                     DIITSANLEQIKKCLKNHETTVIDESKLALFYKAEVFFISTFRKASEKDKKKIKRIHY
                     SESLQEDLISRDFTINAIAMNNGSMLTDALNGYRDLNRKVLRAIGNPRQRFKEDPVKI
                     LRAIRLISELGFRPDFKTANALSSRAKYLKDLELNYSVLREIKKIFEGEHLKLAIKHI
                     EKAKIYKYIPFFQKPIKRLINRYIDIDFETLAIASFVANKQIPKVFENFFDNIKEVEQ
                     LTNLVVATNKAEFPDLVLYTYGLDACLKVNNANLLLSKRYMRPRKLKAKYDKLPIKGT
                     CDLAFKGEDILVLTNNVSGSFLPMIVEEMAHKVINKELDNDREELKTYAIARLNEILG
                     HKKEYNVFKSIYKE"
     gene            5661..6251
                     /locus_tag="PLQ22_02145"
     CDS             5661..6251
                     /locus_tag="PLQ22_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOH58794.1"
                     /translation="MKRITEAISNQIIIKKSEFICTLIPVNNVSEAQEQLAALRKKYY
                     DATHNCYAYIVEDGIKYSDDGEPSQTAGLPIYNVLEKNELTNILAVVTRYFGGIMLGA
                     GGLVRAYTKSTTEALAVANIVEINTYQKILITIDYSKVDILINKLNDEIIEREFGKDV
                     KMTYLILENQVEPFINQVRELTNGDLTIELLEKSTF"
     gene            complement(6278..6511)
                     /locus_tag="PLQ22_02150"
     CDS             complement(6278..6511)
                     /locus_tag="PLQ22_02150"
                     /inference="COORDINATES: protein motif:HMM:NF018335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HOH58795.1"
                     /translation="MDEVTFAIVKLVWFIVILVVVALVTYRALGAVDYSKIFKARSTW
                     QIRTLVLLVSIIVGGLVAFIFLEFIGLLQNVFK"
     gene            complement(6504..7022)
                     /locus_tag="PLQ22_02155"
     CDS             complement(6504..7022)
                     /locus_tag="PLQ22_02155"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOH58796.1"
                     /translation="MEQNKKAKIYTKQGDNKETRTVAGKVLKSDLVIFVEGAVDELQT
                     SIMLARSFLNEVKYRDYLEKITRLLFTLSYSIVSEKDLIEKEDILDLEKEIDLLDSKL
                     PPLNDFILPGFSKAAALVHNARTTTRKTERLVVKYALEHKVDPKILQYLNRLSDYFFV
                     LGRYVDLGENNG"
     gene            complement(7009..7638)
                     /gene="upp"
                     /locus_tag="PLQ22_02160"
     CDS             complement(7009..7638)
                     /gene="upp"
                     /locus_tag="PLQ22_02160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOH58797.1"
                     /translation="MEGKVVIFNHPLMHHKLTLIRDEKTCTKDFRETVEEIAMLMAYE
                     VTRNLPTVEKKIKTPIATMKGQVLAKDVVIVPIIRAGIGMVDGILRLVPTAKVGYVGV
                     YRDDKTLLPHEYYVKLPEKLDQATILVVDPMLATGGSANHTINLLKKRNAKDISFVGI
                     VGAPEGIKVLQDNHPDVNIFLAALDEKLNDNGYIVPGLGDCGDRLYGTK"
     gene            complement(7705..8286)
                     /locus_tag="PLQ22_02165"
     CDS             complement(7705..8286)
                     /locus_tag="PLQ22_02165"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH58798.1"
                     /translation="MEELSLKEFLNLGKKDLKGKVVVFPTDTIYGIGALIDDYDGIKK
                     IFDLKQRPYNQPLANLCFSIEQVKQYVVKIPNSVKEILAVDWPGALTVIFQKKPELEL
                     PFNKETVSFRMPLSIVALKILKNFGPLATTSINIHGTKELNDLVSIKEAFGLQIDYII
                     TDKEIFSSVPSTVIDATGEELKVLREGQIKIRA"
     gene            complement(8274..>8760)
                     /locus_tag="PLQ22_02170"
     CDS             complement(8274..>8760)
                     /locus_tag="PLQ22_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58799.1"
                     /translation="LETAYWFKPFKINLPALIVSFFIALAIVSLIVIIYFYRKKKGVF
                     QKKDESDFDFEKFVKSDEFDNFLSKEIEKPIFEIKPEDVTVEEVTEPVYKRYERDVEA
                     EFSNFNIKEYLEKEGFSTDYTQLNHETKNQITLRLMYLKDQNLITVDDYLKEISKLWK
                     S"
BASE COUNT         2847 a         1492 c         1028 g         3393 t
ORIGIN      
        1 tgcttctatt attaaagcat ctgttgcttc taataatgaa gtatattttt cagggaaaat
       61 acctgtatca gttgcaatca ttaacgtctt ttcttgatga ataaaataat aaccaaaaca
      121 aggaaaatca tgtactagtt gtaaaggaac aacaaaaaag tctaaaatat tatataaagc
      181 atctgcttca attactttaa tatttcctaa agaattatag tatttatatt tatgatctaa
      241 atatttatga gtatcgtaac ttgcataaac tgttgcttta gtttgcttta aaaattgcgt
      301 taaaccataa atatgatcaa tatggctatg tgttaaaaaa atagcatcaa cttcacttaa
      361 gtcaatccct agtttttctg ctcttgtttt agcattacta taggcaattc caacatcaac
      421 taaaatctta tgattttgtg tttcaatata aatgcagttt ccttttgatc cgctagctag
      481 cactacaaac ttcatcttca ttttctcctt attttaatta tataatgttt accttgtttt
      541 ttcaagaaaa aaggcattag atatctagtg ccttttctat atttattaat cagcaagatg
      601 tgctttataa attaaagctt cttgtccttc ttttaatact attcctaaga atttttgata
      661 atctgcgttt gtgaagtctg cttctttaaa atcaaatgtt aatccttttt gttttgaagt
      721 taaaatatgt tgattatttt taactgcatc ttctttaata ccttttaccc agaattcaac
      781 aatctttttc tttgcggcag ctttagttgt atctgtatct aatgcttcac ccgctaaata
      841 ttttttaacc atgtcgaatg taggtaagat atgttcaaca tctccttcag tataagtttc
      901 aagtggtgta gtttttgaat ttacatatac gtgatatcca aattctgtaa ttaaatgttc
      961 accaacaact gtatttacac caaaaatctt atcttcagca ctatcatcat cttcatccca
     1021 aattttctta atgccttcgt tgaatgcttc aaccattcca ccagattgtc ctggaagttt
     1081 gaactcaaat gaacctaaac tttcatattt aacttttaag cctgcagtct tgaatttaga
     1141 aatatcaatc atatcatcta aatcgtattc taattcatta attgtaaatt caggctgaga
     1201 agctgcatct tgaggtaagt gtgcttggaa taatggtgct tcattaaacg cagttacaat
     1261 cttatcaaat tgagctttgt aatcgccttt agcaactaag taagcttgta cttgagcata
     1321 taattcttcc gcaagctcaa cttgttttgg agtccaaaat tttggatcta ataatacttg
     1381 gctagaactt gttgattttt cttcatcaac aacatggatt aataaatgat aaccttcaac
     1441 tttataatat ttactcttaa cattttccat attttttgaa tagtatttag tccaaattgg
     1501 atcagtctcg ctaattaaag agaaatctgt ataaggctta ctagcttcag ctgataattg
     1561 ctcatataat aatctaactt ttacttcttg ttcattgttt gcttggaatc tatatttcat
     1621 ataatctgtc caagtcatat acattgaata attagcatat tgagcttttt catcagtaaa
     1681 tttagtttca atttttgccc atgcttcttt atctaatact ttttcttttt ctacatcata
     1741 aatcttatta atgtcaggat tgttaagtat tctcttaaaa ttgatttcat taattgcaat
     1801 attcattcca taatcttgat ttaatttatg atagaaatct tctactgtat attcaatatc
     1861 tccaagtttc gcaattaaat ctttacttct tgctttgctt gtttcaaatt caattttata
     1921 agtatcagct tgtgatttgt aataagtttc aatttcttta tcataaagaa ctaagtctgc
     1981 agtagcacgt aatccataca ttgcttttgt tacataagca gcagtaagtg tttcttcttt
     2041 taatgcttcg ataactttat cttgtacatc ttctaaagct ggtttttcaa tagttttaat
     2101 ttttagaata gttgcaaatt tcttaccacc atctaaagac ataaaatagt ttttaaacca
     2161 tctttgatct ttattaacaa ctgatgtttc tggatcataa gctttgaaat tcataacatc
     2221 tgataatact gcagcaccaa ttgcctttaa ttcttcatca gtatatagta aagcattctt
     2281 tggatcatca cttacatttc catcttcatc tttaataact tcttgatgga aaacataatg
     2341 accatcttca ttaattgtgt attgtacacc ttcttttaat gtaagagttt catctggata
     2401 accttctact ttataactat gtaaagtatt atataaatca ataaaagctt taattgtttc
     2461 ttctctagtt aattcctctt cagcttgttc ttcatttgga atcttcatcc atttattata
     2521 agaatcagga attaaagctc cacttgcatc tttttcttga tctttaattt tatatcctag
     2581 ctgttctaag gcaccatatg catctttacg agtagcatat tctaaaacaa ttacaaaata
     2641 ttgttcttta tataatgcat tatatttagt atcaatcttt gatttaggaa aatatttttc
     2701 tgtatcgtct tctgcttctg catctttctt agcaatttca tcttctaagt atttagctgc
     2761 ataagctttc ttggcaagtt ctaagcgata gtaatcttta atttccgctt cagtttttaa
     2821 gccttgaaca tatctttcgt tataatattt ttcttctgct tctgcttttt cttctgcttc
     2881 tgctttatct aagtctaatc ctttatacat ttctttttca agttcttctt gaactgcttc
     2941 atctgtaact tctgctagat aattcttttc accattttct aataattcac gatctaacat
     3001 ttcgaataaa gtatttattc catattgatc ttttaataat gaatacattt ctccttttgt
     3061 tagagaatat tcattatctc cttcacctac taatacgtat tgaccatctg gatctgcaat
     3121 cattggtcct ttacgattat ttttagcaac tgttgaagca actgcgataa caacaattac
     3181 caaaacaact aataaaacag gcaaagtact taataatata gttttaggac ttaactttgg
     3241 tgcgtccttt tgcttatttg ccttgctttt tactcttctt cttcttttca tattaaaaac
     3301 acctctttgt atttcacaat atcaatattt tatcacaatt ttactttaat ttcaagcatt
     3361 atattactta atctaaatct ggcaaataaa atattctctt attaaaagca aaaactggtt
     3421 gactgtattc gcctttaact ccatctttta actctttttc taaagcaccc attcttgagt
     3481 cagcatagtc acataaaaac acaacatatg cttccggtat cgttggttct tttggtgaac
     3541 caaattctaa gaaaccatga tgagcaataa taatatgagc tagcatcaaa tattcttctg
     3601 attcctctaa acttaactct tttgcttttt catataatat attagaagca ataataatat
     3661 gtcctaataa attgccttgt ttcgtatatt ctcctgtagt tttttccatt tcggtaatct
     3721 taccaaaatc gtgtaataaa atcccgccat aaattaagtc tttatttaga aacggatata
     3781 actcaatata tgtatcagct aacttaagca ttgaataaac atgataagct agtccagaaa
     3841 tataattatg atgaactgat ttagctgctg gatgaacaaa gtatgcatct aaatcttctt
     3901 ttaaaactgc caatactaac tttttaatat tagcattttc aatttttaaa acatagttca
     3961 taatcttttc ttttaattct tcaactgata acttggcata ttcaaaaaac tcttctaatc
     4021 tatataaact tggatcagct tcagctaatt cttttaagtt aaattgtagt ttaccttgat
     4081 aatctttcat ttgaccagaa caagtataga ttttaccatt ttctaaattc tctctaatac
     4141 tatcagttac attccaaatt ttaacttcta aaatttcttc accatcaaag cctaaagcat
     4201 taacgtagtc tgcttgactt gtagttttgc gatattgaac atttactaac cttaaaatta
     4261 aattattaac ttcatcgcct gcgcgataat ctttaacttt cgttctcatg ttttactcct
     4321 ttattcttta taaatgcttt taaaaacatt atattctttt ttatgaccaa gaatttcatt
     4381 tagtctagca attgcataag tctttaattc ttcacggtca ttatctaatt ctttatttat
     4441 aactttatgt gccatttctt ctacaatcat tggtaaaaaa ctacccgaaa cattgtttgt
     4501 tagaactaag atatcttcac ctttaaaagc taaatcacaa gttcctttaa taggcaactt
     4561 atcatatttt gcttttaatt ttcttggtct catatatctt tttgatagca ataaattagc
     4621 attgtttact ttcaaacaag catctaaacc ataagtatat aaaactaaat caggaaattc
     4681 agctttattt gtagctacaa ctaaattagt taattgttca acttctttga tattatcaaa
     4741 aaagttttca aaaactttag ggatttgctt attagctaca aaactagcta tagctaatgt
     4801 ttcaaaatca atatcgatat acctattaat taatcgctta attggttttt gaaagaaagg
     4861 aatatattta taaatctttg ctttttcaat atgtttaatt gctaatttaa gatgttcccc
     4921 ttcaaaaatt ttcttgattt ctcttaaaac cgaataattt aattctaagt ctttcaaata
     4981 tttggctctt gaacttaaag cattagctgt tttaaaatct ggtctaaacc ctaattcact
     5041 gattaaacga attgctctta aaattttaac tggatcttct ttaaatcttt gtctaggatt
     5101 accaattgct cgtaagactt tacgatttaa atcacgatat ccattcaaag catcagtaag
     5161 catactgcca ttattcatag ctatagcatt tattgtgaag tctctggaaa ttaaatcttc
     5221 ttgaagactt tcagaatagt gaattctctt aatcttcttt ttgtcttttt ctgaagcttt
     5281 tctaaaagta ctaatgaaaa agacttctgc tttatagaat aatgctaatt tactttcatc
     5341 aattactgtt gtttcatgat ttttcaaaca ctttttaatt tgctctaaat tagcactagt
     5401 aataatatct atttctgaaa aaggtatatc taaaataatt gaacgaacta ccccacctac
     5461 aaaatacgct tggtaaccat tcgtgtttaa aacagttaga atttctttac ctttattaat
     5521 gtatcctaaa ttggtagaca ttcagatcac cttcttccca ttatgtatta tatcaaaaaa
     5581 taatcatctt ttccatagta attacttatt ttaacttgag tttttttctc ataacctata
     5641 taattaaatt aggtgataaa atgaaaagaa taactgaagc aatttctaat caaatcataa
     5701 ttaaaaaaag cgaatttatc tgtacattaa ttcccgtaaa caatgtaagt gaagcccaag
     5761 agcaattagc tgctttacga aaaaaatatt atgacgcaac tcataactgc tatgcttata
     5821 ttgtggaaga tgggattaaa tattctgatg atggagaacc aagtcaaaca gcaggattac
     5881 ctatctataa tgttttagaa aaaaatgaac ttacaaatat cttagctgta gttacaagat
     5941 attttggagg gatcatgctt ggtgcagggg gattagttag agcctatact aaatctacaa
     6001 ccgaagcctt agctgttgct aatattgttg agataaatac ttatcaaaaa attctaatta
     6061 caattgatta ttcaaaagta gatatcttaa ttaataaatt aaatgatgaa attattgaga
     6121 gggaatttgg aaaagatgta aaaatgactt atcttatttt agaaaatcaa gttgaacctt
     6181 ttataaatca agttagagaa ctaacaaatg gtgatctaac tatagaatta ttagaaaaaa
     6241 gcacatttta aaatgtgctt tttgtttata attaatttta cttaaaaaca ttttgcagta
     6301 agcctataaa ctctaaaaag ataaaagcta ctaaaccacc tacaataatt gaaactagta
     6361 aaactaaagt tctaatttgc caagtagagc gtgctttaaa tattttcgaa taatcaacag
     6421 cccctaaagc tcggtaagta actagcgcca caacaactaa aattacgata aaccaaacta
     6481 acttaacaat tgcaaatgtt acctcatcca ttgttttcac ctaaatctac atatctaccc
     6541 aaaacaaaga aatagtctga taaacgattt aaatattgta agatctttgg atcaacttta
     6601 tgctccaaag catacttaac aactaatctt tctgtttttc ttgtagttgt acgtgcatta
     6661 tgaactaaag cggcggcttt agaaaaccct ggtaaaataa aatcatttaa tggaggtagt
     6721 ttgctatcta gtaaatctat ctctttttct aaatctaaga tatcttcttt ttcaattaag
     6781 tctttttcgc ttacaatcga ataactaaga gtaaataaaa gcctagtaat cttttctaaa
     6841 taatcgcgat attttacttc atttaaaaat gaacgagcaa gcataattga tgtttgtaat
     6901 tcatcaactg ccccctctac aaaaataact aaatcacttt ttagaacttt accagctact
     6961 gttctagtct ccttgttatc cccttgttta gtataaattt ttgctttttt attttgttcc
     7021 ataaagacga tccccacaat ctcctagccc tggaacgata tatccattat cattcaactt
     7081 ttcatctaaa gctgctaaga aaatatttac atctgggtga ttatcttgta aaactttgat
     7141 cccttctggt gcgcctacga tacctacaaa actaatatct tttgcgtttc ttttctttag
     7201 taaattaata gtatgatttg cacttcctcc agttgctagc attgggtcta caactaaaat
     7261 tgtagcttga tctaactttt ctggtaattt tacgtagtac tcatgaggta ataaagtttt
     7321 atcatctcga tatacaccta catatcctac ttttgcagtt ggaactaatc ttaaaattcc
     7381 atcaaccatt ccgatacctg cacgaatgat tggtacaatt acaacgtctt tagctaatac
     7441 ttgacctttc atagttgcaa ttggcgtttt aatctttttc tctactgttg gtaagtttct
     7501 tgtaacctca tatgccataa gcatagcaat ctcttcaact gtttcacgaa aatcttttgt
     7561 acatgttttt tcatctctaa tcaatgtcaa cttatgatgc ataagtggat gattaaagat
     7621 gactactttt ccttccatat ttggcacctc tttcttttat aaattataac acaaaacgat
     7681 tcattttcaa agaaaaaaag agcattaagc tcttattttt atttgtccct cacgaagcac
     7741 ttttagctcc tcgccagttg catcaatcac tgttgatggc actgaagaaa atatttcttt
     7801 atcggtaatt atataatcta tttgtaagcc aaaagcctct ttaatactga ctaaatcatt
     7861 aagttctttt gtaccatgaa tattaattga tgtagttgca agtggaccaa aattctttaa
     7921 tatttttaaa gcaactatag ataatggcat tctaaatgac acagtttctt tattaaaagg
     7981 tagttctaat tcaggctttt tttggaaaat aactgttaaa gctcctggcc aatcaacagc
     8041 taaaatttct tttactgaat taggaatctt cactacatat tgctttactt gttcaattga
     8101 aaaacacaag ttagctaatg gttgattata aggtctttgt tttaaatcaa agattttttt
     8161 gattccatca tagtcgtcaa tcaaagcccc gataccataa atagtgtcgg ttgggaatac
     8221 tactactttg ccctttaaat cttttttgcc taaatttaaa aattctttta agcttaactc
     8281 ttccataatt tgctgatctc cttcaaataa tcatcaacag ttattaaatt ttgatctttt
     8341 aaatacatta atcttaaagt aatttggttt tttgtttcat gatttaattg agtgtaatct
     8401 gtagagaaac cttctttttc taaatattct ttaatattaa aatttgaaaa ttctgcttct
     8461 acatctctct catagcgttt atatactggc tcagtaactt cttcaactgt aacatcttct
     8521 ggtttaattt caaaaatagg cttttcaatt tcttttgaaa gaaagttatc aaattcatcg
     8581 ctcttaacaa atttttcaaa atcgaaatcc gattcatctt tcttttggaa aactcctttt
     8641 ttcttgcgat aaaaataaat aataacaatt aaactaacaa tagctaaagc aataaagaaa
     8701 cttacaatta acgctggtaa attgatttta aaaggtttaa accaataagc agtctcaaga
//
LOCUS       DAOSGS010000031         8736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6860_length_8736_cov_4.324617, whole genome shotgun sequence.
ACCESSION   DAOSGS010000031 DAOSGS010000000
VERSION     DAOSGS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6860_length_8736_cov_4.324617"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLQ22_02175"
     CDS             <1..863
                     /locus_tag="PLQ22_02175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH58800.1"
                     /translation="TVHDTANPNGGVDAHGAYFRNTTNVSIHYCVGDGRIISSLPEGY
                     RARHAGDGTGTQFKWLDTTITGTGTPVIDINAQGYFTINGQATPLEAPRGRSGEVLDK
                     SYFGDLGPAWKLEGGKYYLGNTRFSTSQTARGVISNYGGNNNSIGIEMCVNTSGNIID
                     TWQRNAKLVADILTRHNLDTNRVKMHNTFDGKNCPSSLRQTNYWAEFMEMVEIEYAFM
                     NEFKDVEVTMVSNTPELLTNLGGIKAKPKNTSTVSYTITVKKGDVTKSVTLSSIVPGI
                     TTWAQLNGFY"
     gene            988..1494
                     /locus_tag="PLQ22_02180"
     CDS             988..1494
                     /locus_tag="PLQ22_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH58801.1"
                     /translation="MFDEIRLKRSMEKLQQGNDEAFEYIYDSTHRLVYYQIYSILKSH
                     QEAENIMQDVYIKVYRNIDKYKNDNPRAWIVTIARNEAINQYHKNKKEVFMDEEELDA
                     FSEEDEKTPLINLAVKILDEEEFLLVAYSVLEGKSRREVAKILDLSPSGVTYKLNLAL
                     EKLKKEVA"
     gene            1496..2743
                     /locus_tag="PLQ22_02185"
     CDS             1496..2743
                     /locus_tag="PLQ22_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58802.1"
                     /translation="MRKLKKELKQEAKIFVPNVKAEVLESVGIREKKKKSYTRILVPV
                     LAVFLVFLTIPLFLSEVNAKETLITVNINPAFLIKAEDDKVKSVEPLNEEAILVLYDE
                     ELNDDLIETIKQLVIKAKELGYENTPRITAYNNNKKNENKMNRHLREQLPNCNISYDE
                     KVSSKERLYQNKEMRERLRGLKLEDAIKTNNGYNQENLENLKQILNQYEQEISNLNYI
                     KKANDLNGAINQFILDMDQAISENDEKKILNRFRNFNNNVMCFGNLGYNKDEIIAYYH
                     KNKGQIQNYTHYVEEETNNYLHQMRKQVVLYLQNENYEVVELDEIVYLPKDLTDVISP
                     LEEYFNFDYNKAFIDHLIATMSRRMQVVEKRGRAFSHELFFIEEQYTILNNYLQENQT
                     YLTDEDVSLFLEKYELFMNKYRR"
     gene            2747..4165
                     /locus_tag="PLQ22_02190"
     CDS             2747..4165
                     /locus_tag="PLQ22_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58803.1"
                     /translation="MKHFKKIIIPVLVLVLTLVLVGCTNSERNASYVTVDINPSVEVI
                     SNKKGIVISVNALNDDAQVLLEGETLEGKTTADVVVEIADLAIKLGYLREGGKVCITA
                     SADSEEIEAKVNGAINKAMNKFKEQKQFAFEVVDGAKQIGRDIVKKAEELGVTAGKYR
                     LILRAMAFDSSLTFEAAKEMSAEELNAIVKAGREELKDLYSEELKKAYQYMKSFSEQT
                     KNAFAFGVLHFLVNTPFFNDKLEEVLAEYDVTIDQVKDILNTYSAELTEVGSELQALM
                     EEYYQIRLELDTNAELVLLLEEKVTLKLELKAAVKAYKANPTDENEAAVVAARAAVEA
                     KQEEILAKKVEIINNLESTITVIIEDGKLVFAGLEAALFEVQEITQKYVEMFEELGIE
                     LDELMVNFRGQFKAFDIFGRFDNAISECEGKYKQFKNQHQAKMNEFKEKVQEEKELRM
                     EFHLAVQARKGSGNNAGKKAGK"
     gene            4306..4974
                     /gene="rpiA"
                     /locus_tag="PLQ22_02195"
     CDS             4306..4974
                     /gene="rpiA"
                     /locus_tag="PLQ22_02195"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOH58804.1"
                     /translation="MNKKKRAGVKACDFVEDKMIVGLGTGSTAYYMIEELGRRKLDIK
                     VVATSNQTKKLALEFGLNLLELEEANHIDLTIDGVDEIDPAFNAIKGGGGALLYEKIV
                     ATNSSKVIWIMDDSKLKDALGEFYLPVEVIEFGHNFVFRKFEKLGLNPKIRTKDNKVF
                     KTDSNNIIIDLYLEKPIDIKKIKTILNNTAGVVEDGLFLNVCDLIVVGYDDKVEVIEN
                     KRKK"
     gene            4987..5517
                     /locus_tag="PLQ22_02200"
     CDS             4987..5517
                     /locus_tag="PLQ22_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOH58805.1"
                     /translation="MNIWHNIDKKRIKEEDFIAVIEITKGSKTKYELDKETGMLILDR
                     VLYTSTHYPANYGFIPRTYCEDDDPLDVLVLASEPLAPMSMVRCYPIGVVRMLDNNEI
                     DDKIIAIPFEDPTWNNYKDISELSPHIESEIMHFFSVYKSLEKKPTAAITSHGKEKAV
                     KIIEEAIAAYKEKFGA"
     gene            5517..6317
                     /locus_tag="PLQ22_02205"
     CDS             5517..6317
                     /locus_tag="PLQ22_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOH58806.1"
                     /translation="MFGLDAKLSTVLGIALIFIATTGGSALVYLIKGKLPDKVFKGIM
                     GFASGVMMSAAIFSLLIPALDLSLDKYEGLKFPYFPAIIGLILGMIFLMVIDSLVPHL
                     HPDLEKEGPKSNLKQSTMLFLGISLHNIPEGLSVGLAFGVGLASGNYALITSAAMLSV
                     GIALQNFPEGAAVSLSYRQAGYSKTKSFLFGMFSGLVEPIFAVLGILLATLFTSIMPW
                     LLAFAAGAMIYIIIEELMPSSVDKENHSHVGVVSFFVGFVVMLIIELL"
     gene            6379..6672
                     /locus_tag="PLQ22_02210"
     CDS             6379..6672
                     /locus_tag="PLQ22_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58807.1"
                     /translation="MKYCPKCQIEVDEKTANCPHCNEPLIIRTNPGNPNEAAENVKGI
                     NEFVVFASLVIPFLGFILYYIWKEKKPYQSKFYLGIGIVSLICYVVMIMILYL"
     gene            complement(7009..8385)
                     /gene="mgtE"
                     /locus_tag="PLQ22_02215"
     CDS             complement(7009..8385)
                     /gene="mgtE"
                     /locus_tag="PLQ22_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOH58808.1"
                     /translation="MNTNTTLKIKHIIQKNSQAKIKNLLGKYHDYDIAITFEILSEDE
                     RQKLYKSLSAQKLSDIFAYLEEPKTYFDELDEEKTAEIISLMDTVDAVDVLKKLDEDD
                     RENILELIDDESAEEIDTIYQYEEETIGSIISTNYITINRNDTIRAAMKKMVTEAAEN
                     DNIFILYVLNDDGTLYGAVDLKDLIIARADSNLEDIIKTNYPILHVNDLIEDKIYKIV
                     EYDLDIIPVLDENEVFVGVLESDNIIDAYQEEVHEDYAKFAGLTEETDIEESIWSTIK
                     KRVPWLIFLLFLGFFVSFLISGFENIIITIPVLVFFQSMLLDMSGNVGTQSLAVTIIG
                     LNEPQQPKWKKVLGKSFVTGILLGLLVALISFISIFAFISITKTTMISEQPFSYPLAL
                     KVSGIISFSMLIAIIIANLTGILIPLTLSKLKIDPAVASGPLITTLNDLITVLVYYGL
                     ASLLLTIL"
     gene            8519..>8736
                     /locus_tag="PLQ22_02220"
     CDS             8519..>8736
                     /locus_tag="PLQ22_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HOH58809.1"
                     /translation="MKYSFLNDYNTLVHPEVFKHLQKYVNEVNGAYGLDEHSKNAKQM
                     ILEKIGNNYDVHFLVGGTSANKIVLSHFL"
BASE COUNT         3261 a         1055 c         1508 g         2912 t
ORIGIN      
        1 taactgttca cgatacagct aatcctaatg gtggcgttga tgcccatgga gcatatttta
       61 gaaatactac taatgtatca attcactatt gtgtgggtga tggcagaatc atttcttcac
      121 ttcctgaagg atatagagca cggcatgcag gtgatggtac tggtactcaa tttaagtggt
      181 tggatactac aattacaggt acaggaacac cagtcattga tattaatgca caaggatact
      241 ttacaattaa tggacaagca acaccacttg aggcgcctag aggaagaagt ggagaagttt
      301 tagataaatc ttattttgga gacctaggac cagcttggaa actagaaggt ggtaaatatt
      361 acttaggtaa tacacgtttt agtacatcac aaactgctcg tggagttatt tctaactatg
      421 gtggtaataa taatagtatt ggtattgaaa tgtgtgttaa tacatctggt aatattatcg
      481 atacatggca aagaaatgct aagctagtag ccgatattct aacaagacac aatttagata
      541 caaatagagt taaaatgcat aatacatttg atggcaagaa ctgcccatca tcattaagac
      601 aaactaatta ctgggctgag tttatggaaa tggttgaaat tgaatatgcc tttatgaatg
      661 aatttaaaga tgttgaggta acaatggttt ctaatacacc agagttacta acaaatctag
      721 gaggcatcaa agcaaaacct aaaaatacaa gcacagtttc atatacgata actgtgaaga
      781 aaggtgatgt gactaaatca gttacactat catctatcgt tccaggaatt acaacttggg
      841 ctcagcttaa cggattttat taatcaaaac taaaagcccc gattggggct tttttaatga
      901 aaataaatat tttactgttc aaaacaggtt ttaaattgtt atatatgtag aaggtagtct
      961 tttacataca ataaaaagga gaattaaatg tttgatgaaa ttagattaaa aagaagtatg
     1021 gaaaaactac aacagggtaa tgatgaagcc tttgaataca tatatgactc aacacataga
     1081 ttagtctatt accaaatata tagtattttg aaaagtcatc aagaagcaga aaatattatg
     1141 caagatgttt atattaaagt ttatcggaat attgataaat ataagaatga taatccacga
     1201 gcttggattg ttacaatagc gagaaatgaa gcgataaacc agtaccataa aaacaaaaaa
     1261 gaagtgttta tggatgaaga agaacttgat gcattttctg aagaagatga gaaaacacca
     1321 ttaattaatt tagcagtgaa aatcttagat gaagaagaat ttttacttgt agcctacagt
     1381 gttttagaag gtaaaagtag aagggaagtt gctaagatct tagatttaag tcctagtggt
     1441 gttacttata aattaaattt agctttagaa aaattaaaga aggaggtggc ataaaatgag
     1501 gaaacttaaa aaagaactaa aacaagaagc taaaatattt gtcccaaatg taaaagctga
     1561 agttttagaa agtgttggca tcagagaaaa gaaaaagaaa tcatatactc gtattttagt
     1621 accagtactt gctgtgttct tagtttttct tactattcca cttttcttaa gcgaagtaaa
     1681 tgcaaaggag acgctaatta ctgtaaatat taatcctgct ttcttaataa aagcagaaga
     1741 tgataaagtt aaaagcgttg aaccattgaa tgaagaagct attttagttt tatatgatga
     1801 agagttaaat gatgacttaa tagaaacaat taaacaatta gtaataaagg cgaaagaatt
     1861 aggttatgaa aacacacctc gcataactgc ttataataat aacaagaaaa atgaaaataa
     1921 aatgaatcgt catttaagag agcaattacc aaattgcaat atctcttatg atgaaaaagt
     1981 aagctctaaa gaaagattat atcaaaacaa agagatgaga gaacgtctaa gaggtttaaa
     2041 attagaagat gctattaaaa ctaataatgg ttataatcaa gaaaatttag aaaacttaaa
     2101 acaaatatta aatcaatatg aacaagaaat tagtaattta aattacataa agaaagcgaa
     2161 tgacttaaat ggagcaatta atcaatttat actggacatg gatcaagcta ttagtgaaaa
     2221 cgatgaaaag aaaatattaa acagatttag aaattttaat aacaacgtta tgtgctttgg
     2281 taaccttggg tataataaag atgaaataat tgcttattat cataaaaata aaggacaaat
     2341 tcaaaattat acgcattatg ttgaggaaga aactaataac tatttacacc aaatgagaaa
     2401 acaagtagtt ttatatttac aaaatgaaaa ttatgaagtt gttgagttgg atgaaatagt
     2461 ttatcttcca aaagacttaa cagatgttat ttcgccgctt gaagaatatt ttaattttga
     2521 ttataacaaa gcttttattg atcatttgat tgcaactatg agcaggagaa tgcaagttgt
     2581 tgaaaaaaga ggacgtgctt ttagccacga gttgttcttt atcgaagaac aatacactat
     2641 tttaaataac tacttacaag aaaatcaaac ttacttaaca gatgaagatg ttagtttgtt
     2701 tttagaaaaa tacgaattgt ttatgaataa atataggagg taatttatga aacattttaa
     2761 aaaaatcatt atcccagtat tagtactagt attaacatta gtacttgttg gatgtactaa
     2821 ttcagaaaga aatgctagtt atgtaactgt agacattaat ccttcagttg aagtaattag
     2881 taataaaaaa ggaatcgtta ttagcgtaaa tgctttaaac gatgatgctc aagtattact
     2941 tgaaggagaa actttagaag gcaaaactac tgctgatgta gtagtggaaa ttgcagatct
     3001 tgcaatcaaa ttagggtatt taagagaagg cggcaaggta tgcatcactg cttctgctga
     3061 ttcagaagaa attgaagcta aagtaaatgg tgcaattaat aaagccatga acaaatttaa
     3121 agaacaaaaa caatttgctt ttgaggtagt tgatggtgca aaacaaattg gtagagatat
     3181 tgtaaagaag gctgaagaat taggggttac agctggaaaa tatcgtttaa tattaagagc
     3241 tatggctttt gatagttctc ttacttttga ggcagctaaa gaaatgtcag ctgaagaatt
     3301 aaatgcaatt gtaaaggcag gaagagaaga acttaaagat ttatattcag aagaattaaa
     3361 gaaagcttat caatatatga agtcattttc agagcaaact aaaaatgcat ttgcttttgg
     3421 tgttcttcac ttcttggtta atacaccttt ctttaatgac aaactagaag aagttttagc
     3481 tgaatacgat gtaacaattg atcaagtaaa agacatttta aatacttatt ctgccgaatt
     3541 aacagaagta ggatccgaac ttcaagctct aatggaagaa tattatcaaa ttcgtttaga
     3601 gttagatact aatgctgaac ttgtattatt gttggaagaa aaagtaactt taaaattaga
     3661 acttaaagcg gcagttaaag cttataaggc aaatccgact gatgaaaatg aagcagcagt
     3721 agttgcagct agagcagcag ttgaagcaaa acaagaagag attcttgcga aaaaagttga
     3781 aattattaat aacttagaat caacaattac agttattatc gaagatggaa aattagtatt
     3841 tgctggttta gaagcggcgt tatttgaagt tcaagaaatt acacaaaaat atgttgaaat
     3901 gtttgaagaa ttaggaattg aattagatga gttaatggtt aactttagag ggcaatttaa
     3961 agcatttgat atttttggta gatttgacaa tgctatttct gaatgtgaag gaaaatacaa
     4021 acaattcaaa aatcaacatc aagcaaaaat gaacgaattt aaagaaaaag ttcaagaaga
     4081 gaaagaatta agaatggaat tccatcttgc agttcaagct agaaaaggtt ctggtaataa
     4141 tgctggaaaa aaagctggaa aataattaat aaacttgttt aataaaaaag gctagcaata
     4201 gccttttttg ccttattata aggattgaaa aaaatactat tttttgatat aatatagata
     4261 atcaatgaga tatcattagt aatattattt agaaaggaaa actttatgaa taagaaaaaa
     4321 agagcaggag ttaaagcttg tgattttgtt gaagataaaa tgatagttgg cttaggtacg
     4381 ggctctactg catattacat gattgaagaa ttagggagaa gaaaacttga tattaaagtt
     4441 gtggctacct caaatcaaac taaaaagtta gctttagagt ttggcttaaa tcttttagaa
     4501 ttagaagaag caaatcatat agatctaaca attgatggtg ttgatgagat tgatcctgct
     4561 tttaatgcaa ttaaaggcgg tgggggagct ttactatacg aaaaaatagt tgctactaat
     4621 agttctaaag ttatttggat tatggatgat agtaaactaa aagatgcttt aggtgagttt
     4681 tatttaccag ttgaagtaat tgaatttggc cataattttg tctttaggaa gtttgagaaa
     4741 ttaggattaa atcctaagat tagaacaaaa gataataaag tttttaaaac agattctaat
     4801 aatataatta ttgatttata tttagaaaaa ccaattgata ttaagaaaat caaaactatt
     4861 ttaaataata cagcgggtgt tgtagaagat ggattgtttt tgaatgtttg tgatttaatt
     4921 gtagttggtt atgatgataa agtagaagtt attgaaaata aaagaaagaa ataaaaggag
     4981 tataatatga atatttggca taacattgat aaaaaaagaa ttaaagaaga agattttatt
     5041 gcagttatcg aaattacaaa aggaagtaaa actaaatatg aactagataa agaaacggga
     5101 atgttaattt tagatcgtgt tttatatact tcaacacact atcctgctaa ctatggattt
     5161 attccaagaa cttattgcga agatgatgat ccattagatg ttttagtatt agcatcagaa
     5221 ccattagcgc caatgtctat ggttagatgt tatcctatag gtgttgttcg tatgttagat
     5281 aataatgaaa ttgatgataa gataattgct attccatttg aagatccaac gtggaataac
     5341 tataaagata tttccgaatt atcacctcat attgaaagtg aaataatgca tttctttagt
     5401 gtttataaaa gtttagaaaa aaagcctact gcagctatta cttcacatgg gaaagaaaaa
     5461 gctgttaaga ttattgaaga agctatagcg gcttataaag aaaaatttgg agcataatgt
     5521 ttggtttaga tgctaagtta agcactgtcc taggaattgc tttaattttt attgcaacta
     5581 ctggaggatc tgcacttgtt tatctaatta aaggtaaact tcctgataaa gtatttaaag
     5641 gaattatggg ttttgcttct ggtgttatga tgagtgctgc tatcttttcg ttgttgatac
     5701 cggctcttga tctatcatta gataaatatg aaggtcttaa gtttccttat tttcctgcaa
     5761 tcataggttt aattttagga atgatttttt taatggttat tgatagttta gttcctcact
     5821 tgcacccaga tttagaaaaa gaagggccaa aatctaattt aaaacaatca acaatgcttt
     5881 ttttaggaat atcacttcat aatattcctg aaggtttatc ggttggttta gcttttggtg
     5941 ttggacttgc aagtggtaat tatgctttga ttacatcagc tgcaatgcta tcggtaggta
     6001 ttgctttaca aaattttcct gaaggagctg ctgttagttt atcttataga caagcaggat
     6061 attcaaaaac aaagtcattt ttgtttggaa tgttttctgg gttagttgaa ccgatatttg
     6121 cagttttggg aattttacta gcaactctat ttacaagcat tatgccttgg cttttagctt
     6181 ttgcggcagg tgcaatgatt tatattatta ttgaagagtt aatgcctagt agtgttgata
     6241 aggaaaatca ttctcatgtt ggtgtagtta gtttctttgt tggttttgta gtaatgctaa
     6301 ttatagaatt actttaaaaa taagagcatt tgctcttttt tttaaaaaat atgttataat
     6361 ataaaaaaga ggtggattat gaagtattgt ccaaaatgtc aaatagaagt agatgaaaaa
     6421 acagctaatt gtcctcattg taatgaacca ttgattatta gaacaaatcc tggtaatcca
     6481 aatgaggcag ctgaaaatgt taaaggaata aatgaatttg ttgtatttgc atcgttagtt
     6541 attccatttt tgggtttcat tttatattat atttggaaag aaaagaagcc atatcaatct
     6601 aagttttatt taggtatagg tatcgtatct ttgatttgct atgtagtaat gattatgatt
     6661 ctttatttat aaaagtatca ttaatagtta ataatttagt taatttaata aaaagcaaac
     6721 gttttcttga caaaatagaa aaatctatgt ataatgatag tagatgaaag tcttaatagt
     6781 gttttattct cacctccttt taataaggtt agatttgact tttaatcgtt caagttattt
     6841 tcccctttca ataacttgga cacacgcttt tagttagcct aacgtttctt ttaaattatt
     6901 aactaaaagc tacattgctt taaccaatgg aagatctatc tcaaaaaaac aaaacaaaaa
     6961 aaatgagcag ttaaagaagg ctcatttttt ctttttatgt atattcattt ataaaatagt
     7021 taacaataat gaagctaaac catagtaaac taaaactgta attaggtcgt ttaatgtggt
     7081 aattagtgga ccacttgcaa ctgcagggtc tatttttagt tttgataaag ttaatggaat
     7141 taaaattcca gttaggtttg cgattattat tgcaattagc atactaaagc taataattcc
     7201 agaaactttc aaagctaaag gataagagaa aggttgttcg gaaatcatag tagttttagt
     7261 aatggagata aaagcgaaaa tagaaataaa actaattagg gcaactaata atcctaataa
     7321 aattcctgtt acaaagctct tacctaaaac ttttttccat tttggttgtt gtggctcatt
     7381 aagtccaatg attgttacgg ctaaggattg tgtacctaca ttacctgaca tatcaagtaa
     7441 cattgattgg aaaaagacta atactggtat tgtaataata atattttcaa aaccagaaat
     7501 taagaacgaa acaaagaaac ctaagaataa taagaagata agccaaggaa ctcttttttt
     7561 aattgtgctc caaatacttt cttcaatatc tgtttcttca gttaaacccg caaattttgc
     7621 atagtcttcg tgaacttctt cttggtaagc atcgatgata ttatcgcttt ctagtacccc
     7681 aacaaacact tcattttcat caaggaccgg aataatatct aagtcatact ctacaatctt
     7741 atagatttta tcttcgatta gatcattaac gtgtaaaatt gggtagttag ttttaataat
     7801 gtcttcaaga ttactatcag ctcttgcaat aattaaatct tttaggtcaa ctgctccata
     7861 taatgtgcca tcatcattta aaacataaag aataaaaata ttatcatttt cagctgcttc
     7921 agttaccatt tttttcattg cagctctaat tgtatcattt ctattaattg tgatatagtt
     7981 tgtggagata atactaccga ttgtttcttc ttcatattga taaatagtat cgatttcttc
     8041 agcactttca tcatcaatta actcaaggat gttttctcta tcatcttcat ctaatttctt
     8101 taaaacatct acagcgtcaa ctgtatccat taagctaatg atttcagctg ttttttcttc
     8161 gtctagttca tcaaaatatg tttttggttc ttctaaataa gcaaaaatat ctgaaagttt
     8221 ttgagccgat aatgatttat atagtttttg tctttcatct tcagataaaa tttcaaatgt
     8281 tatagctata tcataatcgt gatatttacc taacaaattt ttgatttttg cttgactatt
     8341 tttttgaata atgtgtttaa tttttaaagt tgtattagta ttcatattta tctcctttct
     8401 ataatatttt actacaatca ccactgtttt tcaagaaaaa ataaatctat gaaactcttt
     8461 acattttaat acaaaaacac ttagaaattt gctataatat agtaaggagt tgattgatat
     8521 gaagtatagt tttttaaatg attataacac cttggttcac cctgaggtat ttaaacatct
     8581 tcaaaaatat gtaaatgaag tcaatggagc atatggactt gatgagcatt ctaaaaacgc
     8641 caaacaaatg attttagaaa agataggcaa taactatgat gttcattttt tggtaggtgg
     8701 cactagtgct aacaagatag tcttatcgca ttttct
//
LOCUS       DAOSGS010000032         8614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_6983_length_8614_cov_5.587452, whole genome shotgun sequence.
ACCESSION   DAOSGS010000032 DAOSGS010000000
VERSION     DAOSGS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6983_length_8614_cov_5.587452"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..716
                     /locus_tag="PLQ22_02225"
     CDS             3..716
                     /locus_tag="PLQ22_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOH58810.1"
                     /translation="MSAGLSKTNATVSMEMLEHAVREDLKLKTKRIFAVIDPIELVID
                     NYPEGKIEYVDVVNNQENDSLGSRKMAFSKYVYINRDDFIEEKPNKKWKRLALGEEVR
                     LMNAYFVLANSVEYDKDGNVTKVHCTYDPETKSGSGFDARKPNGNIGYVEKSTAKKAK
                     FNYFTSLFVPGTEGKDFSERICKNSWTIKEGFVENIGEFKPGDSFQFIRDGYYTVDRE
                     SKEDELVFNETVALKSSYK"
     gene            725..2830
                     /locus_tag="PLQ22_02230"
     CDS             725..2830
                     /locus_tag="PLQ22_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH58811.1"
                     /translation="MSKILANLNPKQQEAVIYNEGPILVFAGAGTGKTRTLTHRIAYL
                     IEEEGISPEHILAITFTNKATREMRERLEQMLDDSYKKVTISTFHAFCVQVLRRYAQL
                     LDYQNNFRIFDDNDQLRIIREILKEKNINKIILPPALAKKQINYYKTFHTKPSMDDIN
                     YDLIVDLIKTYNKQLKFYNAFDFQDLLDKTLELFQKYPHVLEQYQERYQYILVDEFQD
                     TDIIQYNIIKMMAEKYKNIFVVGDDDQSIYGFRGTNYENMNLFQRDFPDFKKIHLEQN
                     YRSSQAILDISNKLISHNEYREEKELFSEREGKKSDVILRQVDDEKDEATYAISLVKR
                     LKDEGYNYSDIAILYRNSSLSRNFETEAIANSVPYKIYGGYSFTERAEIKDSVAYLRL
                     LLDDDQLSFERIYNRPLRGVGKVTYDKIAAFLDEDPSIANAIDKSKDLLRIDIFENLL
                     KLKDTVLKYRDLLEKQDIQVLFNNYLTEVGYFEYLTEKLEDEDKIQNVLELKSILVEL
                     EEDQIFNSNRERLIGLFDYVVLTESKNKKEQGEKDELLLSTIHSVKGLEFKVVILTAI
                     ENGILPYGYVSNLTIQELEEERRVAYVAITRAKDKLYLTHTQRRLSMGRGRMNIIPSI
                     FLQDIFDSVKEANILNREKRNQIREDRDKFNVGDKVSHKTFGTGIIVSINANYGQIAF
                     SGGKGIKKTFLSPKFLTII"
     gene            2953..3912
                     /locus_tag="PLQ22_02235"
     CDS             2953..3912
                     /locus_tag="PLQ22_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58812.1"
                     /translation="MKVLRRLLLITVLAIGGIMLAGCRTNTAKPAAKNQEDVVSYSVV
                     SGLEMLSAGSARKLSLDADAQDEQVITNDLDEINDYLGLVEKFLSSDDAAITHTEGAS
                     ERDGFEKKMTISTMNLTGERTEYVLYYNETSVNVEEDDDELEVEIELEGIVVLGDVEY
                     TLTGEKEIEQEDNEEEYSYKFEIKLDSKNWVKIEYSEELEDAEVEKSFKYEAYNNGVK
                     NEIKIAYENEDDELKLKLYISDELELEIKQEKNELKIEYKTKDPKTGKKVEGEIKVRV
                     VTDANGNTSYEYKVKEKNGGEVTYQKDRDDDDDDDQKNIAFVF"
     gene            3990..4523
                     /locus_tag="PLQ22_02240"
     CDS             3990..4523
                     /locus_tag="PLQ22_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH58813.1"
                     /translation="MSKLDRQIKKLKNKDEDAFVYIYNQTKAAVYSVVMNVLHDRTQT
                     EDVMQEVYITMVKSINSYQENGKFLSWLLTIAKNKAIDHYRRNKNTEVLFDPTEGENE
                     YLLADNNTHETFSLEANYLLSILDADEKEVVLLKIVSDMKFREISDHLQKPLGTVIWL
                     YNKAIKKMQEEAKEKGL"
     gene            4520..5662
                     /locus_tag="PLQ22_02245"
     CDS             4520..5662
                     /locus_tag="PLQ22_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58814.1"
                     /translation="MKKKELEKLIKSSYEEATPDIISKIDFKKIEIEPQKERRKTRKG
                     FASNLAFALSVFVVMMAVLVSSAVFYKPGTPGEQNGETPLEKILEPNLNYQSFESKQS
                     VVSFSSIMGVNLYNTGNSQAQNLRLAAKDKFVIEEDLDNLNNLLSSLSGFLENAIVTV
                     DLEENDPYFDSNYQVNQKVTITTQAGTNVYIVQYTSEVDDDDDDDDEEEIEVEFEGKV
                     ILNPEGAKVTFLLEGKNSQEEGETEVEYKLYQEDDDDNYILFKQETVERKQKFTYEIY
                     FKDKTEIITKMYIGLDYENKTHIFLETEDKIKDSKAKYNIKEEGDYLIGSYEIDYLDD
                     DEEGTIKISYVDGNNQYEITSDDVTITRIKPFSGFIQTRKKLALFT"
     gene            5755..7725
                     /gene="ligA"
                     /locus_tag="PLQ22_02250"
     CDS             5755..7725
                     /gene="ligA"
                     /locus_tag="PLQ22_02250"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH58815.1"
                     /translation="MDYKKRIEELVELLNQYSYEYYVLDKPSISDFEYDKLYQELKEL
                     EEKYHYILENSPTQKVGDFASSELTKIKHEHPMLSLEDVFNKEDIESFLNRIEKESHL
                     HLEYLCELKIDGIASSLTYKKGKYVLGATRGDGVVGENITRNIATILDVPKVLNKSID
                     LEVRGEVYLPIDNFNKINAEREAEGLALFMNPRNAAGGSLRQLDSSITKERGLATFYY
                     TVVEPEKYNLETQADALHFLSELGFKVNPNYKVCKDIKEVMKYIEEVDVLRKKLNYAT
                     DGVVIKVNNFHLQEEIGYTVKSPKWAVAYKFPPEEAETKLIDIEYSVGRTGTINPTAI
                     LEPVLLSGSVIQRATLNNEEFILEKDIRINDYVIIRKAGEIIPEVVSVNKDKRTEQKP
                     FKMITHCPSCNTLLKKEEGIAMHYCPNPNCEAKIKANLVYFASRDAMNIAGLGEKVTE
                     AMFDLGYLKKVTDIYKLKNHKQELVKIAGFGEKSINNLLDAIEGSKANALALVITGLG
                     IKQIGQKAAKTLANFYKSLDNFLEAKYEELILLKDFGSIMANSVLEYLKENKALIKEL
                     QSLGVDPKEEVLDLPLIFLNQKIVVTGKLELFSRNEITDLIEARGGQVTSAVSKNTDF
                     VIVGENPGSKYTKALELGIKVLTEEEFMKLIK"
     gene            7734..>8614
                     /locus_tag="PLQ22_02255"
     CDS             7734..>8614
                     /locus_tag="PLQ22_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH58816.1"
                     /translation="MKYITIKGARNNNLKNIDLQLPKDKFIVMTGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSSYARQFLGGVDKADVDSIEGLSPSISIDQKTTHNNPRSTVGTVT
                     EIYDYLRLLYARIGKAYCPHHNILIKSQTIEEMLEKVYTYIPDSRCIIYSPIVRGKKG
                     THKETFELVKKEGFSKVRVDGENYDLLEEEPNLDKNFSHNIDLIVDRLKVKPENRSRF
                     YASIETALRLADGIIKVDIDGKEEIFSEHFACPICGFSIPKLEPRLFSFNSPFGACSE
                     CNGLGMIQRVGLDLLVT"
BASE COUNT         3367 a          961 c         1586 g         2700 t
ORIGIN      
        1 taattagtgc aggtttatct aaaactaatg ctactgtttc aatggaaatg ttggagcatg
       61 cagttagaga agatttaaaa ttaaaaacaa agaggatctt tgctgttatt gacccaatcg
      121 aacttgtaat tgataactat ccagaaggta agatagaata tgtagatgta gtaaacaacc
      181 aagaaaatga tagtcttggt tcaagaaaaa tggctttttc taaatatgtt tacattaata
      241 gagatgactt tattgaagaa aagcctaata agaaatggaa gagattagct ttaggcgaag
      301 aagtaagatt aatgaatgct tatttcgttc tagctaatag cgttgaatat gataaggatg
      361 gtaatgtaac taaagttcat tgtacttatg atccagagac taaatctggt tcaggctttg
      421 atgcaagaaa accaaatggt aatattgggt atgtagaaaa gagtacagct aaaaaagcta
      481 agtttaatta ctttacatct ttatttgttc caggaactga aggtaaagac tttagtgaaa
      541 ggatttgtaa gaactcttgg actataaaag aagggtttgt agaaaatatt ggcgagttta
      601 agccaggaga tagtttccaa tttattagag atggatacta tactgtagat agagaaagta
      661 aagaagacga gcttgttttt aatgaaacag tagctttaaa atcttcatat aaataggagg
      721 acaaatgtct aaaatactag caaatctaaa tccaaaacaa caagaagctg taatatataa
      781 tgaaggtcca attttagtat ttgctggtgc tggtactgga aagacaagaa ccttaactca
      841 tcgtattgct tacttgattg aagaagaagg tatttcgcct gaacacattt tagcaattac
      901 ctttactaat aaagcaacgc gtgagatgag ggaaagatta gagcagatgt tagatgatag
      961 ttataagaaa gtaactattt caacttttca tgctttttgt gttcaagtat taagacgcta
     1021 tgctcaactt cttgactacc aaaataattt taggattttt gatgataatg atcaattaag
     1081 gattataaga gaaatcttaa aagaaaagaa tattaataag attatcttac ctcctgcttt
     1141 agctaaaaaa caaatcaact attataagac gtttcatact aaaccaagta tggatgatat
     1201 taactatgat ttaattgttg atttaattaa aacatataat aaacaactaa agttttataa
     1261 tgcttttgat ttccaagact tgttagataa aacattagag ttatttcaaa agtatcctca
     1321 tgttttagaa caatatcaag aacgatatca atacatttta gtagatgagt tccaagatac
     1381 agatattatt caatataata ttattaagat gatggctgag aaatataaaa atatttttgt
     1441 tgttggtgac gatgaccaat caatttatgg tttcagaggg actaactatg agaatatgaa
     1501 tttatttcaa agagattttc ctgactttaa aaaaattcat ctagagcaaa attatcgttc
     1561 tagtcaagct atcttagata tttcaaataa gttaatatct cataacgaat atcgtgaaga
     1621 aaaagaactt ttttcagaac gagaaggcaa gaaatctgat gttatattaa gacaagtaga
     1681 tgatgaaaaa gatgaagcaa catatgcgat tagtttagtt aaaagattaa aagacgaagg
     1741 ctataattat agtgatattg cgattcttta tcgtaattcg agtttatcga gaaattttga
     1801 aacagaagca attgctaata gtgttcctta taagatttac ggcggatatt cgtttactga
     1861 aagagcagaa attaaagatt cagttgctta tttaagattg ttacttgatg atgatcaatt
     1921 aagttttgaa agaatttata atcgcccttt aagaggagta ggtaaagtaa cttatgataa
     1981 aattgctgcc tttttagatg aagatccaag tattgctaat gctattgata aaagtaagga
     2041 tttattaaga attgatattt ttgaaaattt attaaaatta aaagatactg ttttaaaata
     2101 tcgtgattta ttggaaaaac aagatattca agttttattt aataattatt taactgaagt
     2161 tggttatttt gaatatttaa cagaaaagtt agaagatgaa gataaaatac aaaacgtttt
     2221 ggaattaaaa tctattttag tagaattaga agaagatcaa atctttaatt ccaatagaga
     2281 aagattaatt ggtttatttg attatgttgt tcttactgaa agcaaaaata aaaaagagca
     2341 aggtgaaaaa gatgagctac ttttatctac aattcactca gtaaaaggat tagagtttaa
     2401 agttgtaata ttaacagcaa ttgaaaatgg aatcttgcct tatgggtatg tttctaactt
     2461 aactattcaa gaacttgaag aagaaagaag agtagcttat gtagctatta caagagctaa
     2521 agataaactt tatttaactc atactcaaag aagactttca atgggtaggg gtaggatgaa
     2581 cattattcct tcaattttct tacaagatat cttcgatagt gttaaagaag ctaatatttt
     2641 aaatagagaa aaaagaaatc aaattagaga agatcgagat aaatttaatg ttggggataa
     2701 agtcagtcat aagacttttg gtacagggat tattgtaagc attaatgcta actatggcca
     2761 aatagctttt tctggtggca aagggattaa aaagaccttt ttaagtccta aattcttaac
     2821 aattatttaa agaaataaaa taaaaaagag tgaaaacgct ctttttttat tttcttttac
     2881 tttttaccaa taaaactaag ttttcgttcg ttatatatag tgtaagaact aaaactaagg
     2941 aggaggtaaa ttatgaaagt tttaagaaga ttactattaa ttacggtatt agccattgga
     3001 ggaattatgt tagcaggatg tagaaccaac actgcaaagc cagctgccaa aaatcaagag
     3061 gatgtagtta gctattcagt agtttcaggt ttagaaatgc taagtgcagg tagtgcaaga
     3121 aaattatcat tagatgcaga tgctcaagat gagcaagtta tcacaaatga tttagatgag
     3181 attaatgatt atttaggact tgtagaaaag tttctatcaa gtgatgatgc ggctataacg
     3241 cacacagaag gggcttcaga gcgtgatggc tttgagaaaa agatgactat ttctacaatg
     3301 aacttaacag gtgaaagaac tgaatatgtt ctttattaca atgaaacaag tgtaaatgta
     3361 gaagaagatg acgatgaatt agaagttgaa atcgaattag aaggtatcgt tgttttaggt
     3421 gatgttgaat atactttaac tggtgaaaaa gaaatcgaac aagaagataa tgaagaagaa
     3481 tattcataca aatttgaaat taaattagat tctaaaaact gggttaaaat tgagtatagt
     3541 gaagaattag aagatgctga agttgaaaaa tcattcaaat atgaagcata taataatggt
     3601 gttaagaatg aaatcaaaat cgcttatgaa aatgaagatg atgaattaaa gttaaagctt
     3661 tatattagtg acgaattaga attagaaatc aaacaagaaa agaacgaatt aaagattgaa
     3721 tataaaacaa aggatccaaa aactggcaaa aaagttgaag gtgaaatcaa agttcgtgta
     3781 gttactgatg ctaatggcaa cactagttat gaatacaaag taaaagagaa aaacggtgga
     3841 gaagtaactt atcaaaaaga tcgtgatgac gatgatgatg acgatcaaaa aaatatagct
     3901 ttcgttttct aaaaatcgca taacaaaaca tattccttat caactgcctc tttttgtgat
     3961 ataataatgt cataagaagg ggcagattga tgagtaaatt agatagacaa attaagaaac
     4021 taaaaaataa agacgaagat gcttttgttt atatctataa ccaaactaaa gctgctgttt
     4081 atagtgtagt catgaatgtt ttgcatgatc gaacccagac agaagacgta atgcaagaag
     4141 tttatattac gatggttaaa tcgattaata gttatcaaga aaacggaaag tttttaagtt
     4201 ggctattaac gatagctaaa aataaggcaa ttgaccatta tcgtcgcaat aaaaatacag
     4261 aagttttgtt tgacccaaca gaaggagaaa acgaatattt actagctgat aataataccc
     4321 acgaaacttt ttctttagaa gcgaactatt tgcttagtat tttagacgct gatgaaaaag
     4381 aagttgttct cttaaaaatt gtaagcgaca tgaaatttag agaaatttct gaccacttgc
     4441 agaaaccgtt agggacagtt atttggcttt ataataaagc cattaagaaa atgcaagaag
     4501 aagcaaagga gaaagggtta tgaagaagaa agaactagaa aaactaatca agagttctta
     4561 tgaagaagca actcctgata ttattagtaa aatagacttt aagaagattg aaatagaacc
     4621 acaaaaagaa agaaggaaaa ctagaaaggg ttttgcttct aatttagcat ttgctttatc
     4681 tgtttttgtc gttatgatgg cggtgttggt atcatcagct gttttctata aaccgggcac
     4741 accgggcgaa caaaatggtg aaacgccact agaaaaaatt ttagagccaa acttaaatta
     4801 tcagtcgttt gaaagcaaac aatcagttgt tagtttttct tcaataatgg gagtaaactt
     4861 atataatact ggtaatagcc aagcacaaaa cttaagatta gcagcgaaag acaagtttgt
     4921 aattgaagaa gatctagata atttaaataa ccttttatca tcacttagtg ggtttttaga
     4981 aaatgcgatt gtgactgttg acttagaaga aaatgatcct tattttgata gtaattatca
     5041 agtaaaccaa aaggttacaa ttactacaca agctggaact aatgtctata tcgttcaata
     5101 tactagtgaa gttgatgatg atgacgatga tgatgacgaa gaagaaatcg aagttgaatt
     5161 tgaaggtaaa gtaatattga atcctgaagg agctaaagta acatttttac ttgaaggaaa
     5221 aaactctcaa gaagagggcg aaactgaagt tgaatataaa ctatatcaag aagacgatga
     5281 tgataactat attttattca aacaagaaac agttgaaaga aaacaaaaat tcacttatga
     5341 aatttacttt aaagataaaa ctgagattat tactaaaatg tatatcggtc ttgattatga
     5401 aaacaaaact cacatctttt tagaaacaga agataagatt aaagattcaa aagctaaata
     5461 taatatcaaa gaagaagggg actatttaat tggtagttat gaaattgatt atttagatga
     5521 tgatgaagaa ggtacaatta aaattagtta tgttgatgga aataatcaat acgagattac
     5581 ttcggatgat gtaacaatta caagaataaa accattttct ggttttatcc aaacgcgtaa
     5641 aaagctagct ttatttactt aattgtttat taaaaaagtc tactatcgta ggcttttttt
     5701 atgcaataat ggctaaatta tggtataata ttatatatgt ttaggagcat ttagatggat
     5761 tataaaaaga ggattgaaga acttgtagag ttgttaaatc agtatagtta tgaatactat
     5821 gttttagata agccaagtat atctgatttt gaatatgaca agttgtatca agaactaaaa
     5881 gagttagaag aaaagtatca ttatatttta gaaaactcgc caactcaaaa ggttggagat
     5941 tttgcgtctt ctgagcttac aaagatcaaa catgaacatc ctatgctatc tttagaagat
     6001 gtttttaata aagaagatat cgaaagtttt ttaaatcgaa ttgaaaaaga aagtcatctt
     6061 catttagaat acttatgtga attaaagatt gatggtattg catctagctt aacatacaaa
     6121 aaagggaaat atgttttagg agcaacacgt ggtgatggcg ttgtagggga aaacattact
     6181 cgcaatattg ctaccatttt agatgtacct aaagtcttaa ataaatcaat tgatttagaa
     6241 gttagaggcg aagtatattt accaatagat aatttcaata aaataaatgc tgaaagggaa
     6301 gcagaaggct tagctttatt tatgaatcca cgaaatgcag ctgggggttc tttaagacaa
     6361 cttgattcta gcattacaaa agaaagaggt ttagctactt tttattacac tgtagttgag
     6421 ccggaaaagt ataatttaga aactcaagct gatgctttac actttttaag tgagttagga
     6481 tttaaagtta atcctaatta taaagtatgt aaagatatta aagaagtaat gaaatatatt
     6541 gaagaagttg acgttttaag aaagaagtta aactatgcaa ctgatggtgt agttattaaa
     6601 gtaaacaact ttcatcttca agaagaaatt ggttatacag tgaaaagccc taaatgggca
     6661 gtagcatata agtttcctcc agaggaagca gaaactaaac taattgatat tgaatatagt
     6721 gttggtcgta cagggacaat taacccaaca gctattttag aaccagtttt actttctggt
     6781 tcagttattc aaagagctac tttaaataat gaagaattta ttttagaaaa agatattaga
     6841 attaatgatt atgtaattat tagaaaagca ggagagatta ttcctgaagt tgtaagtgta
     6901 aacaaagata aaagaactga acaaaagcct tttaagatga ttacacattg tccttcatgt
     6961 aacactttac ttaaaaaaga agaaggtatt gcaatgcact attgccctaa tccaaattgt
     7021 gaagcaaaaa taaaagctaa tctagtttat tttgcttctc gtgatgcaat gaatattgca
     7081 ggtttaggag agaaagttac ggaagcaatg tttgatttag gatacttaaa gaaagtaaca
     7141 gatatctata aattaaagaa tcataaacaa gagctagtaa agatagctgg ttttggcgag
     7201 aaatcaatta ataatttatt agatgcaatt gaaggatcta aagctaatgc tctagcatta
     7261 gttattacag gacttggaat caaacaaatt ggtcaaaaag cagcgaaaac attagctaac
     7321 ttttataagt ctttagataa ttttttagaa gcaaaatatg aggaactaat tttattgaaa
     7381 gattttggtt caattatggc taatagtgtt ttagaatact taaaagaaaa caaagcttta
     7441 attaaagagc ttcaaagctt aggtgtagat cctaaagaag aagtattaga tttaccttta
     7501 atctttttaa atcaaaaaat tgttgttact ggtaaattag aactattctc aagaaatgaa
     7561 attactgatt taattgaagc aaggggtggt caagttacta gtgctgtatc taagaatact
     7621 gattttgtaa ttgtagggga aaatcctggt agtaaatata ctaaagcatt agaattagga
     7681 atcaaagttt taactgaaga agaatttatg aaactaatta agtaggagaa aagatgaaat
     7741 atattacgat aaaaggggca agaaataata atctaaaaaa catagattta caattaccaa
     7801 aagataaatt tattgttatg actggtgttt ctggatccgg taaatcttca ctagcttttg
     7861 atacgattta cgcagaaggc caaagaagat atgtagagag cttgtcaagc tatgctcgcc
     7921 agtttttagg tggtgtagat aaagctgatg ttgactcaat agaaggtttg agtccttcaa
     7981 tttcaattga ccaaaaaact actcataaca atccaagatc aactgttggt acagttacag
     8041 aaatctatga ttatttacgt ttgttatatg caagaatagg taaagcatat tgtcctcatc
     8101 ataacatttt gataaaaagt caaacaattg aagagatgtt agagaaagtt tatacttata
     8161 ttcctgattc aaggtgtatt atttatagcc caattgttag ggggaaaaaa ggaactcata
     8221 aagaaacatt tgagcttgtg aagaaggaag gctttagtaa ggtacgagtt gatggcgaaa
     8281 attatgattt attagaagaa gaacctaact tagataaaaa tttcagtcat aatattgatt
     8341 tgattgttga tagattaaag gtaaagccag aaaatagaag taggttttat gcttcaattg
     8401 aaactgcttt aagattagct gatggcatta ttaaggtaga tattgatggt aaggaagaaa
     8461 tcttttctga acattttgct tgtcctattt gtgggtttag tattcctaaa ctagaaccaa
     8521 gactattttc ttttaactca ccttttgggg catgtagtga gtgtaatggt ttagggatga
     8581 ttcaaagagt tggtttagat ttgttagtaa ctaa
//
LOCUS       DAOSGS010000033         8482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_7147_length_8482_cov_4.600095, whole genome shotgun sequence.
ACCESSION   DAOSGS010000033 DAOSGS010000000
VERSION     DAOSGS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7147_length_8482_cov_4.600095"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLQ22_02260"
     CDS             complement(<1..1534)
                     /locus_tag="PLQ22_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58817.1"
                     /translation="MKRFLFVLLALLLVGCSCQPEVEELEPFKGFSLPSELDEETAYS
                     LPNTLTYDGKEYNITYDASSLFGEDNVIIGVNEDKVVTISAEVEIDGKTYTFNHQITI
                     KNIIDPFKNFDIIAATESGALVTFPKTLTYEGKDYLVTYTFNPELTDEGRVDPQLPDS
                     QMTIEAKVTINGVDYKATYIILINRATIDPFTNLYMPEVVYSKQALGLDEQVNYEGVN
                     YPVTYKFTPDIIEYSIAPVVEEDIVVAVNASVIINDIKYAKEFNITITRPETSGPEFD
                     EVLKSLPIPNVITTERHIELQKVVLIEDLEFIIDYQSSDESLLTNEGKVVAGKGAKTV
                     DLTASITHLGETYSHTYTINIAAVTATEVLNSINLPEEVDDNIDIPTEFRGYHIDWFS
                     DNERVLSSTGVLSFVSEPTTVNLNAMILDDEMPDRDYVIIAKPFNAQRRLNAALPLIS
                     VPAYVTRSLRFELPYDVKYSWQASTGGVIFEDGVIVRGEQEIQTTLTLTLTAGEETMA
                     MNY"
     gene            complement(1796..3421)
                     /locus_tag="PLQ22_02265"
     CDS             complement(1796..3421)
                     /locus_tag="PLQ22_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58818.1"
                     /translation="MDKTDIANKEPKKEKKCLSFKEIVGYVVENFSKNRLMHFFIVVI
                     FSFSILVFTLMATIILYTNTRNLTRYYNAFEPNYMVFSQEVEYIDELGNNYQKSLVKG
                     QILQKNIKKAFGYTYGVLKGQEVCDIEGDFLGKINLGMSHSTAWDFRLEGNYPLGETD
                     VALPDHFMERAALNIGDEIKINDIQLTITGKILTTYKDVDFSNRYLDQNPNFIYKYNF
                     EYNLGIVNEKYVDLLADNTYSIELNGDITKHDYFGSYNYYTLEYGTINKFEEDVSLKL
                     GSMPVLDNEVMVSETVYSKINKPLYYGISFTLPNLEEEKYHNAHSDVLNLYNFYESGM
                     TIVGVYEDSEHLPDVLITNANFQKIKEEYFKYYAYDNYLNYLNEGYGDIISKLNENSL
                     IWEDPGAKAVLVFSNLKYKLHFGFYLALFLLAVVTTALLVVVFRQNFIDNKDMVKTLI
                     KEGASKKDIYWIFLFKAMAIGSIAMILSMLTLIITLIQINQNMQVNFDIEFNIIRFRY
                     PLYFANLLNTMLLLVGSAHLLAKKLIKSLDNGK"
     gene            3590..4627
                     /locus_tag="PLQ22_02270"
     CDS             3590..4627
                     /locus_tag="PLQ22_02270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOH58819.1"
                     /translation="MDIKKIDLNMATNYLSVDRVNWIQVNNDNFVLKGGLFFKENKSF
                     HRLPKTIETRPNLIALGKHSSGIYFDFKTNSTAISLKVDILSKAYMAHMAATGQNGFD
                     LYFKNKGKWIFLATTKVNFESYALTLIKDLKPALREYRLYFPLYNEVKDIYLGIDEDA
                     SIKKIASKEERIVFYGTSITQGGCATRPGINYTSLLGRKLNLEAINFGFSGNCHLDKA
                     MLDILKEIKNMKYLILEPESNLTNEMLEEKLPPFLKELKNSLKKTEIILISAFPQPVT
                     LIKDEAKENYLKRKEFQKQLCLDLNVRFLDGFGILKKLNYEETVDGIHLTDLGFYNLY
                     LYLNRYLRNLK"
     gene            4624..5484
                     /locus_tag="PLQ22_02275"
     CDS             4624..5484
                     /locus_tag="PLQ22_02275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH58820.1"
                     /translation="MMFTYTIQKQTSIDDFLKSFYLSRSNIYILKTNKLIKVNGKLLA
                     DSILNQGDVVEIDLSSFDTNVAKKKEGKLDIIFENEDFIIINKQKDILTHTDSKSIDT
                     LENFLSYYLDSKVRIVQRLDKETTGIILFPKHLLSASYFSNLIENGRFLRVYLALVKG
                     KMPKQEGYINLPIGKDRHKQNKYLVTKQGKEALTRYKVLEEGNVSLVLFEIYTGKTHQ
                     IRVHTAHIGNPIIGDSLYGNKEDSLYLHSLKIGFVNHRSKQLETYETEIPKRFYERIK
                     KENGNTFRNK"
     gene            5459..6196
                     /locus_tag="PLQ22_02280"
     CDS             5459..6196
                     /locus_tag="PLQ22_02280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOH58821.1"
                     /translation="MEILLETNKYLIGITKEPKYLEQSFKLRFEDLILEFNKTKKANG
                     TDYSKYDNFCEHLVCIDKETDEVVGSYRIATKKTLKSVPFVSEEEFNMDNLKANYNLL
                     EASRAVVKKEYQSTIVLSLLWQAVINYALDNDMDFLFGTASFKGIDPSPYKQAFSYLY
                     YNYLIDEEIMPKATKNKFNLNFIPKDQIDIKTVKEKMPVILKGYCLLNAKVAAEGYID
                     YDFNSVDVLVLLDVKNVNQRILKRFVK"
     gene            complement(6240..7112)
                     /locus_tag="PLQ22_02285"
     CDS             complement(6240..7112)
                     /locus_tag="PLQ22_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58822.1"
                     /translation="MKSQISYIDNLTQPKHGYIEPSKFKKIQYNDSLKLEEEKFSPII
                     VDLTVDYLTRFMTGIPINKAFQVPIEGYINLIVYKAAIKTKNGLLIAMGLEEPNEEVL
                     SIVSEMQAFEKKEGKSIDCFLNQITGLDDKSIFAACKASIYDLRYKNFDYTNYPIYIG
                     EIEPDQTTINNIRVMVNRTINFFKQQGVVEKVGFRCCSDGNIENINSGMSNYLIKDTM
                     WDLKTLKRPISCKNTLKLFINWLNGQKSNNYKIKNVNKIGLFNPRLNTAYLLEISKIS
                     KETIEIISKEVMHK"
     gene            complement(7156..8160)
                     /locus_tag="PLQ22_02290"
     CDS             complement(7156..8160)
                     /locus_tag="PLQ22_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58823.1"
                     /translation="MRKIKLAISVYTDFAYLQYKPPVPKTRSVLARIRNVKQPPNGYV
                     SRKQFQEIKFDDGLKIKEGNISSNFMHTVVNYLTRYMSGVPVPEAFKISYLGYTRRIE
                     FKSYPKEKANLMKIKAMENKYFGYDEEVEIVTEEEESALKEEKKEIIKSNLKFDDDTK
                     IGFKYLSEQITGLDDASIIAACKLCTYDIWFRNLNGVISSPVASDINPDQRSIESIRI
                     MVKRTLKFFDKYGPIIVSGFDFKPNGYTRIINSGDGDYLTKDTLWDLKSIKIPISSQH
                     TLRVVIYWIMGKHSGNEKFENINKIGIFNPRLNIAYILETSQIPKETIKKIEEDVICY
                     "
     gene            complement(8250..8326)
                     /locus_tag="PLQ22_02295"
     tRNA            complement(8250..8326)
                     /locus_tag="PLQ22_02295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8290..8292),aa:Arg,seq:cct)
BASE COUNT         3006 a         1306 c         1087 g         3083 t
ORIGIN      
        1 cataattcat tgccatcgtt tcttctcctg ctgttaatgt tagagtaagt gttgtttgta
       61 tttcttgttc accccttaca ataacaccat cttcaaaaat tactccaccc gtactagctt
      121 gccagctata ctttacatca tatggaagtt cgaatcttaa agagcgagtt acataagcag
      181 gaacgctaat aagcggaagt gccgcattta atcttctttg agcattaaat ggcttggcga
      241 taattacata atcgcgatca ggcatttcat catctaaaat cattgcattt aaattaactg
      301 tagttggttc acttacaaaa ctaaggacac cagttgatga caatactctt tcattatcac
      361 taaaccaatc aatatgatat cccctaaatt cagttggaat atcaatgtta tcatctacct
      421 cttctggtaa attaatgcta tttaatactt ctgttgctgt aacagcagct atatttattg
      481 tataagtatg actataagtt tcacctaaat gagtaatgct agcggttaaa tctacagttt
      541 ttgcaccttt acctgctaca accttacctt cattagttaa caacgattca tcactacttt
      601 gataatcaat tataaattca agatcttcaa tcaaaactac tttttgaagt tcaatatgac
      661 gttcagttgt gattacatta ggaattggca agcttttaag tacttcatca aactcggggc
      721 ccgaagtttc agggcgagta atagtaatat taaattcttt cgcatattta atatcattaa
      781 taattacact tgcatttacc gctacaacta tatcttcttc aacaacaggc gcaatactat
      841 attcaataat gtctggtgta aatttataag taactggata gtttacacct tcataattaa
      901 cttgctcatc taaacccaaa gcttgctttg aataaacaac ctccggcata taaagattag
      961 taaacggatc aatagttgct cgattgatta atattatata tgtagcctta taatcaaccc
     1021 cattaattgt tacttttgct tctattgtca tttgactatc tggaagttga ggatcaaccc
     1081 taccctcgtc agttaactct ggattaaaag tgtaagtaac tagatagtct ttcccttcat
     1141 aagttaatgt ttttggaaaa gtaactaaag ctccactttc ggttgccgct ataatatcaa
     1201 aatttttaaa tggatctatt atatttttta ttgtgatttg gtgattaaaa gtataagtct
     1261 taccatcaat ttctacttct gcactaatag ttactacctt atcttcatta acaccaataa
     1321 ttacgttatc ctctccaaac aaagaagaag catcgtaagt tatattatat tctttaccat
     1381 catatgttag agtgttaggt aaagaatatg ccgtttcttc atcaagctca gaagggagac
     1441 taaatccttt gaaaggttct aattcctcaa cttctggctg acaactgcat cctactagca
     1501 ataaggctag taaaacaaac aagaatcgtt tcataagtta agctccttta tcacttatat
     1561 tattatacca tattttcatc ttttttgcca ccttttagag cctatattac aaacaattta
     1621 catctataaa aaggaaaagc caaactttca taagaaaatt gggctttcgt aggggtgtga
     1681 atattaaata ttcacttagg ggataaagca actatctcta gttgctttat tataatacta
     1741 cactttattt aaaaaaacaa gtaaattatt aaaaaatatt ttattttact tttatttatt
     1801 ttccattatc taaactctta attagtttct tcgctagtaa atgtgccgaa ccaactaaca
     1861 ataacattgt attaagtagg tttgcaaagt aaagcggata tctaaatcga attatattga
     1921 actcaatatc aaaattaact tgcatattct gattgatttg aatcaaagtt ataattaaag
     1981 taagcattga aagaatcatt gctattgaac ctattgccat tgctttaaaa aggaaaatcc
     2041 aataaatatc ttttttacta gctccttctt taattaaagt tttaaccata tctttattat
     2101 caataaaatt ttgcctgaaa actacaacaa gcaacgctgt tgtaactaca gctagtaaaa
     2161 acaaagctaa ataaaaacca aaatggagtt tgtatttcaa attagaaaat accaaaactg
     2221 ctttagcacc cggatcttcc caaattaaac tattttcatt aagtttagaa ataatatctc
     2281 cataaccttc atttaaataa tttaaataat tatcataagc ataatattta aaatattctt
     2341 ctttaatttt ttgaaaatta gcattggtta ttaaaacatc aggcaaatgc tcactatctt
     2401 cgtatacccc aacaatcgtc attccgcttt cataaaaatt atataaattc aaaacatcac
     2461 tgtgagcatt atgatatttt tcttcttcta aattaggaag cgtaaaagaa attccataat
     2521 ataatggttt gttaatctta ctgtatactg tttctgaaac cattacttcg ttatctaaaa
     2581 caggcatgct tcctagtttt aaactaacgt cttcttcaaa cttattaata gttccatatt
     2641 ctaaagtata ataattataa ctaccaaaat aatcgtgttt tgtaatatca ccatttagtt
     2701 ctatactgta agtattatca gctaataaat caacatattt ttcatttaca atacctaaat
     2761 tatattcaaa attatatttg tatataaagt taggattttg atctaaatat ctattactaa
     2821 aatctacatc tttataagtt gttaagattt ttcctgtaat agttaactga atgtcattaa
     2881 tttttatctc atcaccaata tttaaagcag ccctctccat aaaatgatca ggtaaagcta
     2941 catcggtttc acctaaagga taattgcctt ctaacctaaa gtcccaagct gtagaatgag
     3001 acatcccaag attaatcttt cctaaaaaat caccttcgat atcacaaact tcttgacctt
     3061 ttaaaacacc ataagtataa ccaaaagcct ttttgatgtt tttttgtaat atttggcctt
     3121 ttactaaaga tttttgataa ttattcccca attcgtcaat atattcaact tcttgagaga
     3181 aaaccatata attaggctca aatgcattat agtaccttgt taggtttctt gtattagtat
     3241 ataaaataat tgtagccatc aaagtaaaaa ctaaaatcga aaaagaaaaa attacaacaa
     3301 taaagaaatg cattagtctg tttttggaaa agttctcaac cacataacca acaatttctt
     3361 taaaacttaa acatttcttc tcttttttag gttccttgtt tgcaatatct gttttatcca
     3421 tatctaaaac ccctttgttt ttataaaaac aaaatctttc tccttttggt taacttattt
     3481 tatcacactc catatttatt gtcaattaat ggtttctaaa aaaaccaata ctgattttgc
     3541 tatcaaaatt taagatttta tactataatg tataaagagg tagaaaaata tggatatcaa
     3601 aaaaatagat ttaaatatgg ctacaaatta cttatcagta gatagagtaa attggattca
     3661 agtaaataat gataactttg ttttaaaagg tggtctcttt tttaaagaaa acaaatcttt
     3721 tcatagattg ccaaaaacaa ttgaaacaag gcctaattta attgccttag gtaaacacag
     3781 tagtggaatt tactttgatt tcaaaactaa cagcaccgct atttctttaa aagtagatat
     3841 cttatctaaa gcttacatgg ctcatatggc agctacaggc caaaatggat ttgatcttta
     3901 ttttaaaaat aaaggaaaat ggatcttcct agctactact aaagttaatt ttgaaagtta
     3961 tgccctaacg ctaattaaag acttaaaacc cgcactaagg gaatatcgcc tttacttccc
     4021 tttatataat gaagtaaaag atatctactt agggattgat gaagatgcta gtattaaaaa
     4081 aatagcaagc aaagaagaaa gaattgtttt ttatggtact tctattacac aaggtggttg
     4141 tgctacaaga cctggcatta actatactag tcttttagga agaaaactaa acttagaagc
     4201 aattaacttt ggtttttcag gtaattgtca tttagataaa gcaatgttag atatcttaaa
     4261 agaaattaaa aatatgaaat atttaatttt agaaccagaa tctaatttaa ctaatgaaat
     4321 gttagaagaa aaattacctc catttttaaa agaactaaag aattctttaa agaaaacaga
     4381 aattatttta attagtgctt ttcctcaacc agttacttta attaaagatg aagcaaaaga
     4441 aaattattta aaacgaaaag agtttcaaaa acaactttgt ttagacttaa acgttagatt
     4501 tcttgatggt tttgggattt taaaaaagct aaattatgaa gaaactgtag atggtatcca
     4561 tttaactgat ttaggttttt ataacttata tttatattta aatagatatt taaggaattt
     4621 aaaatgatgt ttacatatac aatccaaaaa caaacttcaa tagatgactt tcttaaaagt
     4681 ttttatttaa gtagatcaaa tatttatatt ttaaaaacaa acaaattaat taaagtaaat
     4741 ggtaaattac tagccgatag tattcttaat caaggggatg ttgtggagat tgatttgtct
     4801 tcttttgata cgaatgttgc aaagaaaaaa gaaggtaaac tagatatcat ttttgaaaat
     4861 gaagatttta ttatcataaa taaacaaaaa gatatcttaa ctcatacaga ctctaaaagt
     4921 attgacactt tagagaattt tttaagttat tatttagatt caaaggttag aatagtgcaa
     4981 cgtttagata aggaaacaac aggaattatt ttatttccta agcatctttt aagtgcatct
     5041 tactttagta atctaattga aaacggaaga ttcttaagag tttatctagc tttagtaaaa
     5101 ggtaaaatgc ccaagcaaga agggtatatt aatttaccta taggaaaaga tcgacataag
     5161 caaaataaat acttagtcac aaaacaaggt aaagaagcac taacaagata taaagtctta
     5221 gaagaaggaa atgtaagttt agttttattt gaaatttaca caggtaaaac tcaccaaatt
     5281 agagtccata cagcgcatat tggtaatccg attatcggcg attctcttta tggaaataaa
     5341 gaagactcct tatatcttca ttccttaaaa attggttttg taaatcatag aagtaaacaa
     5401 ctagaaacat acgaaactga aattccaaaa cgcttttatg aaaggataaa aaaagaaaat
     5461 ggaaatactt ttagaaacaa ataaatattt aattggaata acaaaagaac ctaaatattt
     5521 agaacaatct tttaaactaa gatttgagga tttaatttta gaattcaata aaactaaaaa
     5581 agcaaacgga actgattata gtaaatatga taatttttgc gaacatttag tatgcattga
     5641 taaagaaaca gatgaggttg tgggttcata tagaattgca actaaaaaaa ctttaaaatc
     5701 agtgcctttt gtatcagaag aagaatttaa tatggacaat ttaaaagcaa attataattt
     5761 actagaagct agtagagcag ttgtaaagaa agaatatcaa agcaccattg tactgtcttt
     5821 gttatggcaa gcggttatta actatgcttt agataatgat atggattttc tgtttggaac
     5881 agcctctttt aaaggaatag atccttctcc ttataaacaa gcttttagtt atctttatta
     5941 taactattta attgatgagg aaataatgcc taaagctact aaaaacaaat tcaatttaaa
     6001 ttttattccc aaagatcaaa tagatataaa aacagtaaaa gagaaaatgc cggtcatttt
     6061 aaaaggctat tgtttactta atgctaaggt agcagctgaa ggctatattg actatgactt
     6121 taactcagtt gatgttttag tgttgttaga tgttaaaaat gttaatcaaa gaatcttaaa
     6181 aagatttgtg aaataaagaa aagactgaag aaaatcttca gtcttttttt aacatatttt
     6241 tatttgtgca taacctcttt gctaattatt tcaatagttt ctttgcttat cttagatatt
     6301 tccaataaat aagctgtatt taatctagga ttaaataagc ctatcttatt aacattttta
     6361 atcttataat tgtttgattt ttgcccattc agccaattaa taaataattt taatgtgttt
     6421 ttacaagaaa taggtctttt taatgttttt aaatcccaca ttgtatcttt aattaaataa
     6481 ttactcattc cagaatttat attttcaata ttcccatccg aacagcaacg aaaacctact
     6541 ttttctacaa ccccttgttg tttaaaaaaa ttaattgttc tatttaccat aactctgata
     6601 ttatttatag tcgtttgatc tggttctatt tcacctatat atattggata attagtataa
     6661 tcgaaatttt tatatcttaa atcataaata gatgctttgc atgctgcaaa aatagattta
     6721 tcatctaatc ctgtaatttg atttaaaaaa caatctatac tttttccttc ttttttttca
     6781 aatgcttgca tttcactaac aatacttaac acttcttcgt ttggttcttc aagccccata
     6841 gcaattaata gtccattttt tgtttttatt gctgctttgt aaacaatcaa atttatatat
     6901 ccttctatag gcacctgaaa tgctttattt atgggtattc ccgtcataaa tctagttaaa
     6961 taatcaacag tcaaatctac tataattgga gaaaattttt cttcttcaag ttttagacta
     7021 tcattatatt gtattttttt aaattttgac ggttctatat acccatgctt aggctgggta
     7081 agattgtcaa tataagaaat ttgtgatttc atcatatttt gattctccta aactattatt
     7141 aattaataat tcctattagt agcaaattac atcttcttcg atttttttga ttgtttcttt
     7201 aggaatttgt gatgtctcta atatgtacgc tatattcaat cttggattaa atattcctat
     7261 tttatttata ttttcaaatt tttcatttcc tgagtgtttc cccattatcc aataaataac
     7321 tactcgtaat gtatgttggg atgaaattgg tattttaatg ctttttaaat cccatagtgt
     7381 gtcttttgtt aagtaatctc catctcctga attaatgatt cgtgtatatc cgtttggttt
     7441 aaaatcaaat ccactaacaa ttataggacc atacttatca aagaatttga gtgttctctt
     7501 aaccataatc ctaatgctct caatggatct ctggtcggga tttatatcgc ttgccacagg
     7561 ggaggatatg accccattaa ggtttctaaa ccaaatatca tatgtgcata gtttgcacgc
     7621 cgcaataatt gaagcatcat ctaacccagt gatttgttcg cttaaatatt taaaaccgat
     7681 ttttgtgtca tcatcaaatt ttaaatttga tttgataatc tccttttttt cttcttttaa
     7741 agctgattct tcttcctcag ttactatttc cacttcttca tcgtatccaa aatatttatt
     7801 ttccattgct tttattttca ttaggtttgc tttttctttc ggataagact taaattcaat
     7861 acgtcttgta taacccaaat aagatatttt aaatgcctca gggacaggaa caccgctcat
     7921 atatctagtt aaataattta ctactgtatg cataaaatta gaagaaatgt ttccttcttt
     7981 tatttttaaa ccatcatcaa actttatctc ttgaaattgt ttgcgactta cataaccatt
     8041 aggtggttgt ttaacatttc taattcgagc aagtactgaa cgtgtttttg gaactggtgg
     8101 tttgtattga agataagcaa aatctgtata tacacttata gcaagtttaa tttttctcat
     8161 attgtctcct ataactataa tattataaca caaaaacaaa atatttatat tttaaaagct
     8221 acgtttaata aacgtagctt ttaatttaat ggcgtccgtg gaggaattcg aatccccgac
     8281 ccccgcctta ggagggcgat gctctatcca gctgagctac acggacaaat atcttatttt
     8341 accgcgttat tctcttttat cgttccgcct tttattgctg tatcagtcac actcattaaa
     8401 acattaggga agtgtagttt aattttatct tttaaaaaat ttgagttttt acgattaaga
     8461 aacaaaacaa aaatcacttt at
//
LOCUS       DAOSGS010000034         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_7206_length_8419_cov_4.985414, whole genome shotgun sequence.
ACCESSION   DAOSGS010000034 DAOSGS010000000
VERSION     DAOSGS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7206_length_8419_cov_4.985414"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLQ22_02300"
     CDS             <1..677
                     /locus_tag="PLQ22_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58824.1"
                     /translation="EDHYTGNENVVGQHIVIYQVADSSNNTASFTATIKVNDNEGPVF
                     TGENSYTVNTDETLDLEEVRAGLKAFDAVSGNCFVELHLDNYTFNKTKVGTYNVVFKA
                     TDGANNTTYFTVYVTVRDNIPPVFFIKLNPVTLGTSTPLSLSKVLDMTRSYYGLSTDL
                     VYEEQELVEGENIIHLTSKNQNYELKINYIRTEKKEPSKINDSKKLTFFERIRLFFRR
                     IFGSLF"
     gene            complement(618..902)
                     /gene="yidD"
                     /locus_tag="PLQ22_02305"
     CDS             complement(618..902)
                     /gene="yidD"
                     /locus_tag="PLQ22_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH58825.1"
                     /translation="MNKLMIRLIKFYQRNKPDRKHCRYYPTCSNYAIEAYEKFNFFYA
                     TFLTIKRILKCNPLFRPGYDPVPLSRKEKKALKQATENPPKEQSNSFKES"
     gene            1038..2231
                     /locus_tag="PLQ22_02310"
     CDS             1038..2231
                     /locus_tag="PLQ22_02310"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH58826.1"
                     /translation="MAKIMAVNAGSSSLKFKLLELPEEKVITNGVVERIGLDDAIYSI
                     TVNGKKEKQVLAIKDHAVAVKLVLDDLVKRQIVKCLDEIEGVGHRVVQGGWYFKDSAL
                     VTDDVIKKIEKLSVLAPLHNPAHVVGIKAFQKALPDVPQVVVFDTSFHQTMEPETYMY
                     ATPYEWYEKHHIRKYGAHGTSHKYVAGVCLDLLKKKNAKIVTLHIGNGASLAAVKNGK
                     VVDTTMGLTPLEGIPMGTRSGNIDPTVLQVINEKEGLSIQELIYILNNKSGYLGVSGV
                     SHDSRDVEEAMDAGNKRAKLALDIQFKRIADYIGSYYVLMGGLDAIVFTAGIGENSWY
                     CRQEISRRLKVLGVEIDEEFNKKTRGQLKELSTPKSKVKVYIIPTDEEVMIARDVVRI
                     VKKAK"
     gene            2295..4196
                     /locus_tag="PLQ22_02315"
     CDS             2295..4196
                     /locus_tag="PLQ22_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58827.1"
                     /translation="MKKALLSLFLFCLITLSVEAKDIISDVGKDLEMIVSEKEDLIIS
                     DNLNWNKEGKQTIDYFSKIDSEISQIDLYQEKETKLKEGIVYTKQINKFFNLANYRLS
                     NNFYFLENGTYIVLATKELESYNNTPVVLCFRDDSLIWEYEYSQIGDGGFSSAVVENN
                     KIYVLGFHKQVETYNLILVALSLDGLLQNEKIITGNYDAFPISIYSTGYDLLFAWTSR
                     AHSTGHYQDYSNPNQGIVFGQINYDFTNFSLQKYALDYENLEYQTNAYINGEFYVLAK
                     SKELGTSYLVNVDYRFSQVLAQELDSSKSFKTITSFNNRIIGIIEFDTYFKMKVYDYN
                     FNLKKDLFISKNIYGKDYKQFFLLQNESYLYIVLEVKDIVNGKMEYYFTSYNENFVEK
                     NSFDFSFDTDEFINIKLENNYFYFLGKENNVLDLEAKATIILKEEITEDEDYIYYDSY
                     YLVNDKKLEKIEYFKTEFEELTTKKYINEQEDKYLRLYALSKIERKMKNNILDQTTYD
                     TDKILYFNGKGYLNGLVIPNNYQIKEEGYYVFRQVALDGREQILYFTIQDLSLKDVVS
                     KSPIDNKTEYYVLEERQKEIQFQDSNVRFQSYQKTKYGIFIILFSLPIGVLGGIFLPK
                     LIQRRKKDV"
     gene            4189..6408
                     /locus_tag="PLQ22_02320"
     CDS             4189..6408
                     /locus_tag="PLQ22_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58828.1"
                     /translation="MFRILLSFVSLFFNYNPNQDMLILPKVEERQIIDAKSEYLLYHQ
                     GNNYYFASENKEIVLDNFRFEAIIDEEDLAILGYNYQTKLYFARKYNQNLEKTYEKNI
                     FHDSFEFIKCYKIGDYYYFFGNKNYQNSKQEEKNVLEPEEKYGENIFLLKLDSNFNLI
                     KSKYLGGSLDEKLLDVIFYQDRFYFLGYKDRETGGDFGYIGNVSTRNYLSGIINLDLE
                     LESFEVSKTIDYYCKFIEVDFLGFYNNEFLYFLNPNLSLKAKIDFSDFILHIEKVDSN
                     LVVFTLGKVYLYNIENNTIITKIDYPSYMNSEDFQFKRKGKFLTLNEDDKNYLIDVLF
                     LDEFRLSNYYNPDFEDNYVISSLDGKVELVSIEEEYYFDSLVYGIYPFKYNFQTKAGI
                     DFQILHDREVLLRANVVNNGLYPLGYNLKFTGKAYLNDKIILNNYQLTEEGEYKLDLV
                     GNSGQRKSFYFTVSGKQNKIEDSYYEKYDLEYSLDERIVLDITIDNLENKTIERVIVD
                     GEDVDFRIDLKQNKIFLDLPKYSNPGFYKVKLEKLEYKEEGVIKELVLNKELILKIIV
                     SQINYEIDNSNNPLTIDLTIYDPYGISRFLELVIYNDVEEYRQKLALNNLIIYENRLK
                     KETDYEFKIRLVSTLPGELVYSRELVSGKINANGNELILGEIELEESDLEGKKLKLEL
                     REDFVSKEVRELFYLNQAIYTQEETNYFGIILFSVLIIGASFGITYIALKLIRKAKS"
     gene            6482..>8419
                     /gene="dnaE"
                     /locus_tag="PLQ22_02325"
     CDS             6482..>8419
                     /gene="dnaE"
                     /locus_tag="PLQ22_02325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH58829.1"
                     /translation="MRIASLYNLTEYSLLESTNPISELVNKAKASGYSALAITDTSMS
                     GAFKFYQAALKVGIKPILGLKVPIERENSYLLFYAKNKRGYQELLELDTLQKQKLIEL
                     SDLKATNDLVVVIPHLENDLSRLILTTDIIGANILVRKYQELFSDLYFGLSQTSNLEK
                     DSSEVLLSYFKSLGINAVAISQSRYLKESDLDAYRIVNALRTNNFSYELNSEETNMYF
                     LEKDYFRDLFRKYDETLANTQSIADKVNLELKFEGYLLPAFETPNNDSDEYLDSLCKF
                     GLNKRLRGRHVNQELYVARLLEELETIKSMGFSDYFLVVYDFIRFAKQNDILVGPGRG
                     SAPGSLVAYSLGITEIDPISNGLLFERFLNKERITMPDIDTDFPDNKRELVIQYLGEK
                     YGINKVAHISAFSTFKAKQAVRDVARVLNLDNTSLSEVMKHISSTRPLQESITTSTAL
                     QRLVKENKEIYNVIDLALKIEGLPRHYSTHAAGVVMTDDDLVKHTSLIKSTDNIMQTV
                     YEAEDLEKLGLVKIDILGLRNLTIIEEVIEYIKENEKGEFNLYQIPLNDQEVFKLFQK
                     ADTDGIFQFESSGMRNLLHDMHPDSFSDIVVAIALYRPGPMAMIPEFVSRKKDKRSIT
                     YLHPDLKDILEETYGVIVFQEQ"
BASE COUNT         3216 a          818 c         1438 g         2947 t
ORIGIN      
        1 aagaagatca ttatacagga aacgaaaatg ttgttggtca acatattgtc atttatcaag
       61 tagctgatag ttcaaataat actgcttcat ttacagctac cattaaagta aatgataatg
      121 aaggtcctgt ttttacggga gaaaatagtt acacagttaa tactgatgag acattagatt
      181 tagaagaagt aagggctgga ttaaaagctt ttgatgctgt ttcaggaaat tgttttgtag
      241 aactacattt agataattat acttttaata aaacgaaagt gggaacatat aatgtagtct
      301 ttaaagcaac tgatggagct aataatacta catattttac agtctatgta acggttagag
      361 ataatattcc accagtcttt ttcataaaac taaatccggt tactttaggt acaagtactc
      421 cactttcact ttctaaagtt ttagatatga ctagatctta ttatggttta agtactgact
      481 tagtttatga agagcaagaa ttagtagaag gtgaaaatat tattcattta actagtaaaa
      541 accaaaacta tgagttaaag attaactata ttaggacaga aaaaaaagaa ccatctaaaa
      601 taaatgattc taaaaaatta actttctttg aaagaattag attgttcttt aggaggattt
      661 tcggtagcct gttttaaagc ttttttttcc tttcttgata aaggtactgg atcatatcct
      721 ggtctaaata atggattaca ctttaaaatt cttttaatgg ttagaaaagt tgcgtaaaaa
      781 aaattaaact tttcgtaagc ctctattgca taattggaac atgtagggta atacctacaa
      841 tgcttacgat cgggtttatt tctttggtag aatttaatta atctaatcat taacttattc
      901 atgaaaagca cctcatttat tatattatat aataaagggg tgtttttttc aagaattcaa
      961 ctctgataat aattgaatta ttctttaatc tatggtaaaa taatgggagt gttgagaata
     1021 aagaaagagg tgttaaagtg gctaaaataa tggcagttaa tgcaggtagt tcatcactta
     1081 aatttaagtt attagaactt cctgaagaaa aagtaattac taatggtgtt gtggaaagaa
     1141 taggtttaga tgatgctatt tatagtatta ctgttaatgg aaaaaaagaa aaacaagttt
     1201 tagcaattaa agatcacgca gttgcagtta aattagtttt agatgattta gttaaaagac
     1261 aaattgtaaa gtgtttagac gaaattgaag gtgtaggaca tagagtagtt caaggtggtt
     1321 ggtattttaa agattcggct ttagttactg atgatgttat taaaaagatt gaaaagttat
     1381 cagtacttgc tcctttacat aatcctgccc atgttgttgg gattaaggct tttcaaaagg
     1441 ctttacctga tgtacctcaa gtagttgttt ttgatacatc attccaccaa acaatggaac
     1501 cagagactta tatgtatgca actccatatg aatggtatga aaaacatcac attagaaaat
     1561 atggagctca tggtactagt cataaatatg tagctggtgt ttgtttagat ttattaaaga
     1621 agaaaaatgc taagattgta actttacata ttggcaatgg agcatcacta gcagctgtta
     1681 agaacggtaa agtagttgat acaacgatgg gtcttactcc gcttgaaggt attccgatgg
     1741 gaacaagaag tggtaatatt gatcctactg ttttacaagt aatcaatgaa aaagaaggtt
     1801 tatcaattca agagctaatt tatattttaa ataataaatc tggttattta ggtgtaagtg
     1861 gtgttagtca tgattctcgt gatgttgaag aggcgatgga tgcaggtaac aaaagagcaa
     1921 aacttgcttt agatatccag tttaagagaa ttgcagatta tattggctca tactatgttt
     1981 taatgggtgg cttagatgca attgtcttta cagcaggaat tggtgaaaat tcatggtact
     2041 gtcgtcaaga aatatcaaga agattaaaag ttttaggtgt agaaattgat gaagagttta
     2101 ataaaaaaac tcgtggacaa ttaaaagaat tgtctactcc taaatcaaaa gtaaaagtat
     2161 atattatccc tacagatgag gaagtaatga ttgctcgtga tgtagtgagg attgttaaaa
     2221 aggctaaata atagcctttt ttttaagagt aatttttatt ttcttttaat aatatataat
     2281 taaaggaggt aaaaatgaag aaagcattac ttagtttgtt tttattttgt cttattactt
     2341 taagtgtaga agccaaagat ataattagtg atgtaggtaa agatttagaa atgatagttt
     2401 ctgaaaaaga agatttaatt atatctgata atctaaattg gaataaagaa ggtaaacaaa
     2461 caatagatta tttctctaaa atagatagtg aaattagtca aatagatttg tatcaagaaa
     2521 aagaaacaaa attaaaagag ggtattgttt atacaaaaca gataaataaa ttttttaatc
     2581 ttgctaatta tcgcttaagt aataattttt attttttaga aaatggaaca tatatagtat
     2641 tagctactaa agaattagaa tcatataata atacacccgt agttttatgc tttagggatg
     2701 atagtctaat ttgggagtat gaatatagtc aaatagggga tggtggtttt agttcagcag
     2761 tagtagaaaa taataagatt tatgttttag gatttcataa acaagtagaa acttataatt
     2821 taattttagt tgccttatct ttagatggct tgttgcaaaa tgaaaaaatt attacaggca
     2881 attatgatgc ttttccaatt agtatttatt ctacaggtta tgatttgctt tttgcttgga
     2941 caagtagagc gcattcaaca ggccactatc aagattattc taatcctaat caaggaatag
     3001 tttttggcca aattaactat gattttacta actttagttt acaaaaatat gccttagatt
     3061 atgaaaattt agaatatcaa actaatgcct atattaatgg tgagttctat gttcttgcta
     3121 agagtaaaga attaggtaca agttatctag tgaatgtaga ttatcgtttt tcacaagttt
     3181 tggctcaaga attagattca agcaaaagct ttaaaactat aactagtttt aataatagga
     3241 taattgggat aatagagttt gatacttatt ttaaaatgaa agtttatgat tataatttta
     3301 atcttaaaaa agatttattt attagtaaaa atatttatgg gaaagactat aaacagttct
     3361 ttcttttaca aaatgaaagt tatttatata tagttttaga agtcaaagat atagttaatg
     3421 gtaaaatgga gtattatttt acatcttaca atgagaattt tgtagagaaa aacagctttg
     3481 attttagttt tgatactgat gaatttataa atataaaatt agaaaacaat tatttttact
     3541 ttttagggaa ggaaaacaat gttttagatt tagaagcaaa agcaacaatc attcttaaag
     3601 aagaaattac tgaagatgag gactatattt attatgatag ttattattta gttaatgata
     3661 aaaagttaga aaaaattgag tattttaaga ccgaatttga agagttaaca acaaaaaaat
     3721 acattaatga acaagaagat aaatatttac gactttatgc tttaagcaaa attgaaagaa
     3781 aaatgaaaaa caatatatta gatcaaacta cttatgatac agataaaata ctttatttta
     3841 atggtaaagg atatttaaat ggtttagtaa ttcctaataa ttatcaaatt aaagaagaag
     3901 gttattatgt ctttagacaa gttgcattag atggaagaga gcaaattctc tattttacaa
     3961 ttcaagactt atcgcttaaa gatgtagtaa gtaaaagtcc tatagataat aaaactgagt
     4021 attatgtatt agaagaaagg caaaaagaaa ttcaatttca agattcaaat gtaagatttc
     4081 aatcgtatca aaaaacaaaa tatggcattt ttattattct tttttcactt cctataggag
     4141 tacttggagg tatattttta ccaaaattaa tccaaaggag gaaaaaagat gtttagaata
     4201 ttacttagtt ttgtttcttt gttttttaat tataatccca accaagatat gctaatctta
     4261 ccaaaagtag aagagagaca aataattgat gcaaagagtg aatatttact ttatcatcaa
     4321 ggaaataact attattttgc tagtgaaaat aaagaaatag ttttagataa ttttagattt
     4381 gaagctatta tagatgaaga agatctagca attttaggat ataattatca aaccaaacta
     4441 tattttgcga gaaaatataa tcaaaatctt gaaaaaacct acgaaaaaaa tatttttcat
     4501 gatagttttg agtttataaa atgttacaaa ataggtgatt actattactt tttcggcaat
     4561 aaaaactatc aaaattctaa gcaagaagag aaaaatgttt tagaaccaga agaaaagtat
     4621 ggcgaaaata tctttttatt gaagttagat tcaaatttta atttaataaa atcgaaatat
     4681 ttaggtggta gtttggatga aaaactatta gatgtaattt tctatcaaga tagattctac
     4741 tttttaggat acaaggatag agaaacaggg ggcgattttg gttacattgg caacgtaagc
     4801 actaggaatt atttatctgg aattattaat ttagatttag aattggaaag ctttgaagtt
     4861 tccaaaacta ttgattatta ttgtaagttt attgaagttg actttttagg attttataat
     4921 aatgagtttt tatatttctt aaaccctaat ttaagtttaa aagctaaaat tgatttttct
     4981 gattttattt tacatatcga aaaagtagat tctaatttag ttgtttttac tttgggaaaa
     5041 gtatacttat ataatattga aaataatact ataattacta aaattgatta tcctagttat
     5101 atgaatagtg aagattttca gttcaaaaga aaaggaaaat tcttaacgct gaacgaagat
     5161 gataaaaatt atttaattga tgttttgttt ttagatgaat ttagattaag taattattat
     5221 aatcctgatt ttgaagataa ttatgttatt agttctttag atggtaaagt agaactagta
     5281 agtatcgaag aagaatatta ttttgatagt ttggtttatg gcatttatcc tttcaaatat
     5341 aattttcaaa ctaaagctgg aattgatttt caaatcttac atgataggga agtattatta
     5401 agagcaaatg tagttaacaa tggtttatat cctttaggtt ataatttgaa gtttacaggt
     5461 aaagcatatt taaatgataa aattatttta aataattacc agttaactga ggaaggtgaa
     5521 tataagttag acttagttgg taatagtggt caaagaaaat cattttattt tactgtttca
     5581 ggaaaacaaa acaagattga agatagttat tatgaaaaat atgatttaga gtatagttta
     5641 gatgaaagaa tagttttaga tataacaatt gacaatctag aaaataaaac aattgaaaga
     5701 gtaattgtag atggtgaaga tgttgatttt aggatagatt tgaaacaaaa caaaatattt
     5761 ttagatttac ctaaatatag taatcctggc ttttataaag taaagttgga aaagcttgaa
     5821 tataaagaag aaggagtgat aaaagaatta gttttaaata aggaattgat tttaaaaatt
     5881 attgtttctc aaattaacta tgaaattgat aatagcaaca atcctttaac aattgattta
     5941 acaatctatg atccttatgg cattagtaga tttcttgagc ttgttattta taatgatgtt
     6001 gaggagtata ggcaaaaact agcattaaat aatttaatta tttatgaaaa caggttaaaa
     6061 aaagaaactg attatgaatt taagattcgt ttggttagca ccttaccagg tgaactagtt
     6121 tatagtagag aattagtttc gggaaagata aatgctaatg gaaatgaatt aattttgggg
     6181 gaaattgaac tagaagaaag tgatttggaa ggaaagaaat taaagttaga gttaagggaa
     6241 gactttgttt ctaaagaagt aagagaactt ttctacttaa atcaggcaat ctatactcaa
     6301 gaagaaacta attattttgg gataatttta tttagtgttt taataatagg agcaagcttt
     6361 ggaataacct atattgcttt aaaattaatt agaaaagcta agagttaatc ttagcttttt
     6421 ttattggatt actttataca aatagcctaa tttatggtat aatataaatt gaggaagtat
     6481 tatgaggata gcatcgttat ataatttaac tgaatattct ttacttgaaa gtactaatcc
     6541 aatctctgaa ttagttaata aagctaaagc tagtggatat tctgctttag ctattactga
     6601 tactagtatg agtggagctt ttaaatttta tcaagctgca ttaaaagtgg gaattaaacc
     6661 tattttgggt ttaaaagttc caatagagag agaaaatagt tacttgcttt tttatgcgaa
     6721 aaataaaaga ggatatcaag agttattaga attagataca ttacaaaagc aaaaactaat
     6781 tgagttaagt gatctaaaag ctactaatga tttagttgta gttattcctc atctagaaaa
     6841 tgacttaagt cgtttgattc taacaacaga tattattggt gctaatattt tagtaagaaa
     6901 ataccaagaa ctatttagtg atttgtattt tggattgtca caaacaagca atttagaaaa
     6961 agattctagt gaagttttat tgtcttattt taagagttta ggaataaatg cagttgcaat
     7021 ttctcaatct aggtatttaa aagaaagtga tcttgatgct tatcgtattg ttaatgcatt
     7081 aagaactaat aattttagtt atgaactaaa tagtgaagaa actaatatgt actttttaga
     7141 aaaagattat tttcgtgatt tatttagaaa gtatgatgag accttagcta atacgcaaag
     7201 tatagctgat aaagttaatt tagaactaaa gtttgaagga tatttacttc ctgcatttga
     7261 aacaccaaat aacgatagcg atgaatattt agatagttta tgtaagtttg ggttaaataa
     7321 aagattaaga ggaagacatg ttaatcaaga actttatgtt gctagactat tagaagagtt
     7381 agaaacaatt aagagtatgg gatttagtga ttacttttta gttgtttatg attttattcg
     7441 ttttgctaag caaaatgata ttttagtagg acctggtaga ggttctgcac caggatcttt
     7501 agttgcctat agtttaggca ttacggagat tgatcctatt agtaatggtt tgttatttga
     7561 aagattctta aataaagaaa gaattacaat gccagatatt gatactgatt ttcctgataa
     7621 taaaagagag ttagttattc agtatttagg agagaagtat ggaataaata aggtagccca
     7681 tatttctgct ttttctacat tcaaagcaaa acaagctgta agagatgtag cacgagtttt
     7741 aaacttagac aatactagtt taagtgaagt aatgaaacat atttctagta ctagaccttt
     7801 gcaagaatct attactacaa gtacagcatt acaaagatta gttaaagaaa acaaagaaat
     7861 ttataatgta atagatttag ctttaaaaat tgaagggtta ccaagacatt attcaactca
     7921 tgcagctggt gttgtaatga cagatgatga tttagtaaaa catacttctt taattaaaag
     7981 taccgataat attatgcaaa cagtatatga ggctgaagat ttagaaaagt taggtttagt
     8041 aaagattgat attttaggtt taagaaactt aacgattatt gaagaagtta ttgagtatat
     8101 taaagaaaac gaaaaaggtg agtttaattt atatcaaatc ccattaaatg atcaagaggt
     8161 tttcaaattg tttcaaaaag ctgatacaga tggaattttt caatttgaat caagtgggat
     8221 gaggaactta cttcatgaca tgcatcctga tagttttagt gatattgtag ttgctattgc
     8281 cttatataga ccagggccaa tggcaatgat tcctgagttt gtaagtcgca agaaagacaa
     8341 aaggtctatt acatacttac atcctgattt aaaagatatt ttagaagaga cttatggagt
     8401 tatagtattc caagaacaa
//
LOCUS       DAOSGS010000035         8303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_7354_length_8303_cov_4.437924, whole genome shotgun sequence.
ACCESSION   DAOSGS010000035 DAOSGS010000000
VERSION     DAOSGS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7354_length_8303_cov_4.437924"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLQ22_02330"
     CDS             complement(<1..733)
                     /locus_tag="PLQ22_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH58830.1"
                     /translation="MDFILGLLAFIFVLGLIILVHEGGHMIFAKKAGILVREFAFGMG
                     PAIFKKKVGETEYSIRILPIGGFCAIAGEEQEDDYLEKVQYVKLKFKQGTVTDIYITL
                     NHKDAKDLEMFEIVDYDLYDEQSRGFLFIAVKDSSGNLKKYGVSKSAILHEGKYSMQI
                     APYERTLGAKSLWDRFMVMFGGPLMNFFLAMLVFVILGLGQGFPKYDTNKVNVNAEYL
                     IESGFTSGSYIEKMEISGYSVEVKEY"
     gene            complement(745..1884)
                     /gene="dxr"
                     /locus_tag="PLQ22_02335"
     CDS             complement(745..1884)
                     /gene="dxr"
                     /locus_tag="PLQ22_02335"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOH58831.1"
                     /translation="MRNLYLLGATGSIGTQALNIIRENQDYRVLGISFYNNISLGKEI
                     IKEFKPELVWVKNEEIKRDLEKEFPKVKIYFGKEGMIDFASYKKPGLVLNALTGSSGL
                     LPTYYAIKSKKDILLANKESLVIGGKLIIDLARKMKVSIFPLDSEHNSIYQLLRKENK
                     EDVKKVYLTASGGALRDKNREELKSVTKAEVLNHPNWKMGDKITVDSATLINKGFEVI
                     EAYHLFGLELNQIDTVLHRESIVHALVEYKDSTVLANLSYPNMEIPISYALSYPKRRK
                     TSVASLDLGKLKSLNFEKIDYERYPLLKLSFEVLKKGEAKQIAYSASNEAAVNLFLAG
                     KISFLEIEQIVIEAVKKDYPKFEISLDNILDLDLEIKKEIYEKYK"
     gene            complement(1897..2793)
                     /locus_tag="PLQ22_02340"
     CDS             complement(1897..2793)
                     /locus_tag="PLQ22_02340"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH58832.1"
                     /translation="MSPKIKNLIIRATTGLVYTGLIIGLALVNGPWFMILGILFSILA
                     TYELTNAFSNKHPAYNHLKYIMPLIAGGLTFIFIYFYDDSKIVLLSYFLSLLIVFSLL
                     LFSKDTKIIDYGLGILALVYTSLMVGAIFSLKYINLGITNTFLLNRFNFDGKLIFIYL
                     FIVVVMTDMMAYIFGSLIGKHKLAPTISPNKSVEGAIIGTVLGSLFGLLALVLLKIVD
                     LNALSSKELVLVFIVIFFINILISIVGQIGDLFASKIKRNYEIKDFGKLLPGHGGVLD
                     RIDSLIFGATLIQVILIILTLV"
     gene            complement(2790..3458)
                     /gene="uppS"
                     /locus_tag="PLQ22_02345"
     CDS             complement(2790..3458)
                     /gene="uppS"
                     /locus_tag="PLQ22_02345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOH58833.1"
                     /translation="MKLPRHVAIILDGNGRWAKAKGLPRSFGHQEGLKALVELIKYTN
                     SIGLEELSVFGFSTENWNRPKTEINYIMGLVEKGYKNYQEKIKAMDLKIKVVGELEML
                     TPNLQKIANELEEATKDNKGMILNVCFSYGGKQELVRAFKKLEGQEITIEKIQNNLDI
                     KTDVDLLIRTGGNQRISNFLIWQLAYAELYFTEVYFPDFKKGDFDQAIEYYNKQERRF
                     GGLK"
     gene            3582..4304
                     /gene="pyrH"
                     /locus_tag="PLQ22_02350"
     CDS             3582..4304
                     /gene="pyrH"
                     /locus_tag="PLQ22_02350"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOH58834.1"
                     /translation="MKRIILKLSGEALAYPDSKGIDFQKVNKIALEIKKLYDLGNIQL
                     GIIVGGGNIWRGKDAEELGIDRSSADYMGMLATILNALAIQAALEKLGLDTRATTSLE
                     IPAVAEPYIRRKAISHLEKNRVVIFGGGTGLPFFSTDSTASLRGAELGADIILMAKNG
                     VDGVYDKDPNKYPDAKRYDKLTYEEVIEKNLGVMDLTAATLCKENNIKIIVFDMNKED
                     NIKNVVSNPKIGTVINNRKEKK"
     gene            4304..4867
                     /gene="frr"
                     /locus_tag="PLQ22_02355"
     CDS             4304..4867
                     /gene="frr"
                     /locus_tag="PLQ22_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH58835.1"
                     /translation="MELLQVLLDACEEKMIKAIDALEKEMGLIRTGRANPKVLSGVMV
                     SYYGMSTPLEQIASISTPEAQQLLIKPYDVSALKDMEKAIQLADLNLNPMSDGKVLRI
                     NFPPLTEETRKMHVRELNALLESYKIQMRNFRRESNDEIKKMENDKEISEDASHSYQN
                     EIQELTDKYIKLIEEKGKKKEKDILEI"
     gene            4952..5518
                     /locus_tag="PLQ22_02360"
     CDS             4952..5518
                     /locus_tag="PLQ22_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOH58836.1"
                     /translation="MDLKKFKKYPELKGTQTEANLKEAFAGESQAHTKYLYYASQAKK
                     DGYVQIGKFFEETALNEKEHAKIWFKLLNEGSVPKTTANLEDAASGENYEWTEMYPGF
                     AKTAEKEGFPEIAALFRLVADVEKRHEERYKKLKVNIEEGIVFSRSKDTVWECSNCGH
                     IHIGKEAPDMCPVCDHEKAYFEIHKTNY"
     gene            complement(5601..6437)
                     /gene="tsf"
                     /locus_tag="PLQ22_02365"
     CDS             complement(5601..6437)
                     /gene="tsf"
                     /locus_tag="PLQ22_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOH58837.1"
                     /translation="MAVVISAQLVKELRDRTGAGMMDCKKALVETGGDLEKASVWLRE
                     NGITKAAKKATRVAAEGICSALINGNKTLVYEINCETDFVTKTDKFQAVVEKVGKALL
                     KSKATNTEEALKVKAEEGTVEDLIIQATAIIGEKIDLRNVSVITKKDDEKFEVYSHMN
                     GRIVVVITYKGKEDAAHDVALHVAAMNPTYLSRADVDEAFLAREKAIFRQEALNEGKP
                     EKIVDKIAEGKVNRYLKDICLLDQQFVKNSDITVEQFLKQEETSISHFYRIEVGVGVE
                     RE"
     gene            complement(6920..7576)
                     /gene="rpsB"
                     /locus_tag="PLQ22_02370"
                     /pseudo
     CDS             complement(6920..7576)
                     /gene="rpsB"
                     /locus_tag="PLQ22_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            complement(7978..>8303)
                     /locus_tag="PLQ22_02375"
     CDS             complement(7978..>8303)
                     /locus_tag="PLQ22_02375"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOH58838.1"
                     /translation="KAKKKVQVKTNLKAYMKKDEVLFLFIRSSYAIKKGITLTNNVGI
                     IDSDYFSNEDNDGEIIVSLTNNTDEDIYLKRGDRFIQGIFLKYLTVDGDTTTKVRKGG
                     IGSTK"
BASE COUNT         2765 a         1481 c         1035 g         3022 t
ORIGIN      
        1 aatattcttt tacttcaact gaataaccac ttatttccat tttctcaata taagagccac
       61 tagtaaagcc actttctatt aaatactcag catttacatt aactttgttt gtatcatatt
      121 tagggaaacc ttgtcccaga cctaaaatta caaaaacaag catggcaagg aaaaagttca
      181 ttaatggccc accaaacata accataaatc tatcccataa tgatttagca cctaatgttc
      241 tttcataagg agcaatttgc atactatact taccttcatg aagtatcgca cttttactta
      301 caccatactt ctttaaatta ccagaagaat cttttactgc aatgaataaa aatcctctgc
      361 tttgctcatc atacaaatca tagtcaacaa tttcaaacat ctctaaatct tttgcatctt
      421 tatgatttaa tgttatatag atatctgtaa cagtgccttg tttaaacttt aactttacat
      481 actgaacttt ttctaaataa tcatcttctt gttcttcccc cgctatagca caaaatcctc
      541 caattggtaa aattctaata ctatattcag tttcccctac tttctttttg aagatagctg
      601 gccccattcc aaacgcaaat tcgcgaacta aaattcccgc tttcttcgca aatatcatat
      661 gtcctccttc gtggactaaa ataattagcc ctaagacaaa gataaaggcc aacaacccta
      721 atataaaatc catttaactc acctctattt atatttctcg tatatttctt tttttatttc
      781 taaatctaaa tctaaaatat tatctaaact aatttcaaat tttggataat ctttcttaac
      841 agcttcaata actatttgtt ctatttctaa aaaactaatt ttcccagcta aaaacaaatt
      901 aactgcagct tcattactag cactataagc aatttgctta gcctctcctt ttttcaaaac
      961 ttcaaaacta agttttaata aaggatatct ttcataatct atcttttcaa aattcaaaga
     1021 ttttaacttt cctaaatcta aactagcaac acttgttttt cttctctttg gataagataa
     1081 agcataacta attggtattt ccatatttgg ataagataaa ttagcaagaa ccgtactatc
     1141 tttatattct actaaagcat gaacaataga ttcacgatga agcacagtat caatttgatt
     1201 taattctaaa ccaaacaagt gataagcttc aataacttca aaacctttat tgattaatgt
     1261 agctgaatca actgtaattt tatctcccat tttccaatta ggatgattta atacttctgc
     1321 tttagtaacc gactttaact cttctctatt tttatctctt aaagcaccac cactagcagt
     1381 taaataaacc ttctttacat cttctttatt ttcttttctt aataattgat aaatagaatt
     1441 atgttcgcta tctaaaggaa aaatagaaac tttcattttt ctagcaagat caataattaa
     1501 ttttccacca attactaaac tctctttatt agctaataag atatcttttt tgcttttaat
     1561 tgcataataa gtaggtaata acccactact gccagttaaa gcgtttaaaa ctaaaccagg
     1621 ctttttatat gaagcaaaat caatcattcc ttcttttcca aaataaatct ttaccttagg
     1681 aaactctttt tcaagatctc tctttatttc ttcatttttt acccaaacaa gttctggttt
     1741 aaattcttta attatttctt tccccaaaga aatattatta taaaaactta ttcctaaaac
     1801 acgataatct tgattttctc taattatatt taaagcctga gttccaattg aacctgtagc
     1861 tcctaataga taaagattac gcataatttc tcctttctaa actaaagtta aaattattaa
     1921 aatgacttga attaatgttg ctccaaaaat caaactatca attcgatcta aaactcctcc
     1981 atgacccggt aagagctttc caaaatcttt aatttcataa tttcttttta ttttagaggc
     2041 aaataaatca cctatttgac caacaatact aattaaaata tttataaaaa atattactat
     2101 aaatactaaa acaagttcct tagaacttaa agcatttaaa tctactatct ttaataaaac
     2161 taaagcaagt agaccaaaca aacttcctaa aacagttccg ataatagcac cctcaaccga
     2221 tttatttggg ctgattgttg gggctagttt atgttttcca attaatgacc caaaaatata
     2281 agccatcata tccgtcatca caacaacaat aaataaatat ataaaaatta atttaccatc
     2341 aaaattaaat ctatttaata aaaatgtatt ggtaatacct aaattaatat attttaagga
     2401 gaaaattgct cctaccatta aactagtata aaccaaagct aatattccta aaccataatc
     2461 aataatctta gtgtctttac tgaaaagtaa caaactaaaa actattaata atgatagaaa
     2521 ataactaagt aaaacaatct ttgaatcatc ataaaaataa atgaaaataa aagttaatcc
     2581 cccagctatt aatggcataa tatattttaa atgattgtat gctggatgtt tattactaaa
     2641 agcattagtt agttcatagg tagctaatat agaaaacaaa attccaagga tcataaacca
     2701 aggtccattt actaaagcta agcctataat cagccctgta taaactaaac cagttgtagc
     2761 tctaattatt aagtttttaa ttttcgggct cattttaaac ctccaaagcg acgttcttgt
     2821 ttattataat attctatagc ttgatcaaaa tctccttttt tgaaatcagg aaaataaact
     2881 tcagtaaaat aaagttcagc ataagcaagt tgccaaataa ggaagttact tattctttga
     2941 tttcctccag ttcttattaa taaatcaacg tctgttttta tatctaaatt gttttgaatt
     3001 ttttcaatag ttatttcttg tccttctaat tttttaaaag cacgaactaa ttcttgcttt
     3061 cccccataag aaaaacacac atttaaaatc atacctttat tatctttagt tgcttcttct
     3121 agctcattgg ctattttttg taaattaggt gtaagcattt caagttcacc aacaacctta
     3181 atcttaagat ccatagcttt tattttttct tgatagtttt tatatccctt ttctacaagc
     3241 cccattatat aattaatttc ggttttcggc ctattccaat tttctgtact aaaaccaaaa
     3301 acagatagtt cttctaaacc aattgagtta gtatatttta ttaattcaac taaagctttt
     3361 aatccttctt gatgaccaaa agaacggggc aacccttttg cttttgccca tctaccatta
     3421 ccatcaagaa taattgccac atgtcttggt aatttcataa ctttctccat ttatcatatt
     3481 atactataag tcgtctataa tttccattaa taaatcttta agaaaggaaa aaagtattcc
     3541 ctttttccct tatctttgat ataatataaa aggtgatagt tatgaaaaga ataattttaa
     3601 aattaagtgg tgaagcttta gcttacccag atagtaaagg aattgatttt caaaaagtaa
     3661 ataaaatagc tttagaaatt aaaaaacttt atgatttagg aaatatccaa ttaggaataa
     3721 ttgtaggtgg aggcaatatc tggcgtggga aagacgcaga agaattagga attgatcgtt
     3781 cgagtgctga ctatatggga atgttagcta cgattctaaa tgcccttgct atccaagctg
     3841 ccttggaaaa gttaggacta gatacaagag caacgacatc tttagaaatc cctgcagtag
     3901 ctgaacctta tattagaaga aaagcaatct ctcatttaga aaaaaatcga gttgtaatct
     3961 tcggcggggg tacaggctta ccattcttta gcactgactc aactgcttct ttaagaggag
     4021 ccgaacttgg cgctgatatt attttaatgg ctaaaaatgg tgttgatggt gtctatgata
     4081 aagatcctaa taagtatcct gatgccaaac gctatgataa attaacttat gaagaagtta
     4141 ttgaaaagaa tttaggtgtt atggatttaa ctgctgcaac actatgcaaa gaaaacaata
     4201 ttaaaattat tgtctttgat atgaacaaag aagacaatat caaaaacgta gtttcaaacc
     4261 ctaagattgg aactgtgatt aataacagaa aggaaaagaa ataatggaat tattacaagt
     4321 attattagac gcatgtgaag aaaaaatgat taaagcaatt gatgctttag aaaaagaaat
     4381 gggcctaatt agaacgggtc gtgctaatcc aaaagtttta agtggagtta tggttagcta
     4441 ttatggtatg tctactcctt tagaacaaat tgcttcaatt agtacaccag aagctcaaca
     4501 gttgttaatt aaaccttatg atgtatctgc tttaaaagat atggaaaaag caattcaact
     4561 agctgatctt aatttaaacc cgatgagtga tggtaaagtt ttacgtatta acttcccacc
     4621 gcttactgaa gaaacaagaa aaatgcatgt tagagaatta aatgctttgc ttgaaagtta
     4681 taagatccaa atgagaaact ttagaagaga atctaatgat gaaatcaaaa aaatggaaaa
     4741 cgataaagaa atctcagaag atgcatcaca ttcttatcaa aacgaaatcc aagaattaac
     4801 tgataaatat atcaaattaa ttgaagaaaa aggtaagaag aaagaaaaag atatcttaga
     4861 aatttaaaaa ggagaatatc tccttttttc ttttaagtct tttattttat aaagaataat
     4921 ggtataatat ctatatcgga ggaggaataa aatggattta aagaaattta aaaaataccc
     4981 agaattaaaa ggtacacaaa ctgaagcaaa tttaaaagaa gcttttgctg gggaatctca
     5041 agctcacaca aaatatttat attatgcttc acaagctaaa aaagatggtt atgttcaaat
     5101 aggtaaattt tttgaagaaa ctgctttaaa cgaaaaagaa catgcaaaga tttggttcaa
     5161 gttattaaat gaaggctcag taccaaaaac tactgctaac ttagaagatg ctgcaagtgg
     5221 tgaaaactac gaatggactg agatgtatcc agggtttgca aaaactgcag aaaaagaagg
     5281 tttccctgaa attgctgctt tatttagatt agttgcggat gtagaaaaaa gacacgaaga
     5341 aagatataaa aaactaaaag ttaatatcga agaaggtatt gttttctctc gtagcaaaga
     5401 tacagtttgg gaatgttcta actgtggtca tattcacatt ggcaaagaag cgccagatat
     5461 gtgcccagta tgtgatcacg aaaaagcata ttttgaaatt cataaaacta attactaaaa
     5521 ttaaataaaa taataaaaag gctagattaa ctagcctttt tttattaatt aaatgtgatt
     5581 tttattgggc aatcacacat tcattctctt tcaacaccaa caccaacttc aatacgatag
     5641 aagtgtgaaa ttgatgtttc ttcttgtttt aaaaattgtt ctacagtaat gtcagaattt
     5701 ttaacaaatt gttggtcaag taagcagata tcttttaagt atctattaac tttaccttca
     5761 gcaatcttat caacgatttt ttcaggtttt ccttcgttaa gtgcttcttg tctgaaaata
     5821 gctttttctc ttgcaaggaa agcttcatca acatctgctc tacttaaata tgtaggattc
     5881 atagcagcta cgtgtaatgc aacatcatga gcagcatctt cttttccttt gtatgtaatt
     5941 acaacaacaa tacgaccatt catatgcgaa taaacttcaa atttttcatc gtctttttta
     6001 gtaattactg atacattgcg taaatcaatt ttttctccaa taatagcagt tgcttgaata
     6061 ataaggtctt ctactgtacc ttcttcagct ttaactttta aagcttcttc agtattagta
     6121 gctttactct taagtaatgc ttttcctact ttttcaacaa cagcttggaa tttatctgtt
     6181 ttagtaacga agtctgtttc acagttaatt tcataaacta atgttttatt tccattgatt
     6241 aatgctgaac aaataccttc agctgcaact ctagttgctt ttttagcagc tttagtaatt
     6301 ccattttctc ttagccaaac actagctttt tctaggtctc cgcctgtttc aactaaagct
     6361 tttttacaat ccatcattcc agcaccagta cggtctctca attcttttac aagttgtgca
     6421 ctaataacaa cagccattat tttacctcct ctgttacttc ttctacttta acttctattt
     6481 cttttttagc cttaggggct ttttcttctt ttacttcagc tgcttctttt tttgctctct
     6541 taggttttgc ttcaacttca gcttctgctt tttcagcttt aacaactttc ttaggttttg
     6601 cttcaacaat cacatttaat tctttttcag ctttagcagc ttttttaggt ttttcttctt
     6661 tttctaattt tacttctttt ttgatcgctt gtttttcttt aacttctttg atttcttctt
     6721 tcttagcctt tttcttttct tctagttcaa tttccttagc tttttcagtt ttatcatcta
     6781 caggtaagat taaagcatca acttgtctaa ctcttctagg tcttcttcca cgaccaaaac
     6841 gtccttgttg atgtgcatca gctggttctt gttcttctgg aactacgatt tcaactggtg
     6901 gtaatccttt agcagttgca actgcattag ccataatcgc agtaataact tttactgatc
     6961 taacggcatc atcatttcct gggattacat aatcaacttc atctggatca caattagtat
     7021 ctactaaacc aaataaaggt atattaagtt ttctagcttc aagtacagca ttacgctcac
     7081 tatttggatc aattacaaac atagcttgag gaatcttagt catatctcta attccactaa
     7141 aatatttaac taatttttct tgttcctttt taattaaagc aacttctttt ttagtgtaac
     7201 tttctaaagt tccttctgcg tccattcttt caatatcttc taaacgttta attgaacgtc
     7261 taattgtttt gaagtttgta agcataccac ctaaccatct ttggtctacg tagtattcgc
     7321 cacatctttc agcttcttct ttgataattt cttgaatttg ttttcttgta cctacaaaca
     7381 aaactcttcc gccattttgc acaatagtat aaagtgcttt gtaagattca ttaatacctt
     7441 caattgtttt gtctaggtca atgatataaa taccattctt ttctccaaaa atatactctt
     7501 tcattttagg attccatttg cgagtattgt gaccaaattg gactcctgct tctagtagca
     7561 agttacggct aacgatttct tctgaatgtg cctcttcctt aacttcagct ttggcttcaa
     7621 cctttggttc agctttggct tttggtttta attcaacctt taattcagct tcaggttttg
     7681 cctttggttt tggttttgcc tcaacttcaa ctttagcaac tttcttaggt ttttcttcag
     7741 cctttgcttc tggttttgct ttggttttta cctcagcttt ttcagcttct tgttgctcta
     7801 acaactcttt ttctttcatt tttttcactc ctttttttct tttgttaagc ctccaccctc
     7861 ttcatagatt cccgcccact tcaagctaaa agtgacatga cgattttctc caaggatgtg
     7921 tgtttttaag actaaattat tatatcataa cttaaattaa tttgcaaaca tttcttgcta
     7981 tttagtacta ccaataccac cttttcttac tttagtcgta gtgtcgccat ctacagttaa
     8041 atacttcaaa aatatccctt gaataaaacg gtctcccctt tttaaataga tatcttcatc
     8101 agtattgttt gttagggaaa cgataatttc cccatcatta tcttcgttac taaagtaatc
     8161 actatcaata atccctacat tattggtaag tgttattcct tttttaattg cataactact
     8221 tctaataaac aaaaacaaaa cttcatcttt tttcatatat gctttaagat tagtcttaac
     8281 ttgtaccttt ttcttagctt tta
//
LOCUS       DAOSGS010000036         8299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_7357_length_8299_cov_6.294639, whole genome shotgun sequence.
ACCESSION   DAOSGS010000036 DAOSGS010000000
VERSION     DAOSGS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_8299_cov_6.294639"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLQ22_02380"
     CDS             complement(<1..330)
                     /locus_tag="PLQ22_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH58839.1"
                     /translation="MYEVVVVGGGHAGIEAALATARRNHKTLLIAGNFDHIASMACNP
                     SIGGPAKGIVVREIDALGGEMGKAVDKCLIQLKMLNKSKGPAVRALRAQVDKITYPKY
                     MQEAILKQ"
     gene            complement(416..2416)
                     /locus_tag="PLQ22_02385"
     CDS             complement(416..2416)
                     /locus_tag="PLQ22_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58840.1"
                     /translation="MKKKIAIIFLSIFVLFFTAGCIAKDGSTDIFRFDVHSVTMEVGQ
                     TKVLSITGQFNVESEIEYTITEESKNIIKFEKIANDKVKITALAVGVGNIKAHLVERD
                     IYAPAIIEVTSPKTSGIRISTPEGFNSEMEIGEELQLGYTLTSNTNETTEVTWEVGDE
                     SRASITANGKFTPQFGGNHFIYARTVDGNYAVLKVFVNYHDPTAVVIDVVENKSAAVT
                     PTNNKIAYLREVIKLGSMVAPATASAKVEWRLLHRTDDNQVIADPASVGLVLNQNGEV
                     YCTSLATQQRFRLQAVAMAGDVEIKSNIITLDYTYAPVESVQITQKDVNGKTVAVEHS
                     VTIIAGQEIQFGALVAPANAAATVTWSKKVVNPVFDSSQRFIQDPITRRTITEANLAT
                     ISDKGLFKSTYHGIVEVELSSLADSNIKHTITVHILEKKKPVKLELLMDIDDRLLSYK
                     FYSDSAISTAIDNADNKRKFIIGTVDITAQIFPEETHQDLKLELVEKDMAFVIKDGIT
                     TTSRGVTTFKLGIVKEGILHFKISSQYNALNEYITPIEKALKIISEDFEINSSEGLKE
                     DAFGDQTYQYYEAISFSSIIDKADKEFGIGDAEITEDSWQIEALDGSDINNAKILLYS
                     AGFGKLADFLALGEGKFKITFTPSSYTKPLYTVVINVVDIAK"
     gene            complement(2465..2992)
                     /locus_tag="PLQ22_02390"
     CDS             complement(2465..2992)
                     /locus_tag="PLQ22_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58841.1"
                     /translation="MKKDKTIKINNEEEVTYYKGKYTYRGKSYKIREWYSLCASFRVF
                     NEEEIELRYLPSNISPSYLKETYIKNVKMATFYCVIAPLIFFFLVGLFFLIAPSIIIS
                     EQNSKIYFYVFGAFLILGSFVIFFQHLLGKRSCFIKIRRVNRYRFITKKEYQENLRIF
                     DIYIKKEKEQKEFFS"
     gene            complement(3065..3931)
                     /gene="fba"
                     /locus_tag="PLQ22_02395"
     CDS             complement(3065..3931)
                     /gene="fba"
                     /locus_tag="PLQ22_02395"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOH58842.1"
                     /translation="MALVNMKKMLKKANKEGYAVGQFNINNLEWAKTILLVAQEKNSP
                     VILGVSEGAAKYMGGYNVVMGMVKGLIKDQKITVDVAVHLDHGTTFEACKAAIDAGFT
                     SVMIDASKHPIEENVRLSKQVVDYAHPQGVTVEAEVGIVGGEEDGIVGKVQYAKLEDC
                     IRIVKEAGVDCLAPALGSAHGPYKGVPKLGFDEMDAINKAAKVPLVLHGGSGIPDDQI
                     KKAIARGTAKINVNTELQQEFAKDLRALLADPKNDAVYDPRKILGPCTAGIKRAMLGR
                     MEVFGSIDKAAK"
     gene            complement(3957..4835)
                     /locus_tag="PLQ22_02400"
     CDS             complement(3957..4835)
                     /locus_tag="PLQ22_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH58843.1"
                     /translation="MSKIAIITDVNAGLDYIGYDPQIPTLRSVINFGEEHLVDGIEIR
                     ADEFYERLKKVKSKEDIPSTSAPTLGDMMIMIEDHIKKGYTDIILFSISYKLSSIGQT
                     FMTLKEEYADRINLYSVNTKTATYLQGYYALRAKEMVKEGKSVEEILEYSEYLTQNSH
                     AYFVVDSLDYLVKNGRLGRFSGTLGSWLAIKPILELNEEGAIVTFDKVRTYSKAIERL
                     KELVIGEIGDSQDVVIQVIQSGNIETCKQIVKDLKEYYGNRAEIGLQYITPAVGAHIG
                     RDVVGIGYFILDKFKK"
     gene            complement(4937..5347)
                     /locus_tag="PLQ22_02405"
     CDS             complement(4937..5347)
                     /locus_tag="PLQ22_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58844.1"
                     /translation="MQKSKLIRFLQIIGFIGLAAAILATVMPAISAIIIALKIIILFI
                     VLVVTLGLILFNAEYRALWKDIDYIPLDLFQNIGNILYAVAIVFGILTLVMVILKRKQ
                     LLKFKKYLTTSLIISGVSVLGIIIHYLLYTYVYK"
     gene            complement(5406..>8299)
                     /gene="mfd"
                     /locus_tag="PLQ22_02410"
     CDS             complement(5406..>8299)
                     /gene="mfd"
                     /locus_tag="PLQ22_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH58845.1"
                     /translation="GDFSVRGEVIDIFPILHEKPVRITFFDDEIESIRFFDVKDQLSL
                     EKIEEIDIYPIQELIYNEEQKKEVISKLKKHNNPHIQELLKDVENHKNDERVNKLINY
                     FYDEQASLLDYQNSLTFYDEYNDLKNVYKKILIDIQTQRESLELQIELLYTLEYEAIN
                     KPKQKVYLEQFSKYIDVTLDKLIDFKTYSIIDYQNDLKNLIFEIKLNKQKTYILTFKT
                     TERLKLFKKVIEESLNYLEIESFEDIEENQVHLIVVENSISFGFIDEKLEVISEEDIF
                     KDLSIKKTKKTIAYQGKTIFDKDELKLGDYIVHYDYGLAIYQGIKTVELNGLKQDYLT
                     LKFSNSDLLVPVENINLISKYVGSEGVVPKLNKLGTKSWEKKRKRIKEQLVDIATVII
                     DNESERSKATGYKYLPDDEFQKIIESDFPHEETPDQTAIIENIKAEMEDGKVIDRLVC
                     GDVGFGKTEIALRTAVKTVINNKQAAYLAPTTILSNQHYNTFKNRLEPYGIRVELLNR
                     LVPLRKQREVIEDTKKGLVDILIGTHRILSDDVGFYDLGLLIVDEEQRFGVMHKEKIK
                     TLKKDINILTLTATPIPRTLHIAISGIKSLSLLETPPKSRYPIQTYVLSKNDTIIKEA
                     IYRELNRGGQIFYLHNQISTLHKTAINLKRLVPEAKIVIAHGQMNKEKLEDNVASFIN
                     LEYDILLCTTIIETGIDIPNANTLIIEDADHLGLAQIYQIRGRVGRSDRIAYAFLMYD
                     NKKILTETAYKRLEAIKEFTTLGSGFNIAIRDLSIRGAGDLLGKEQSGFIDSVGLDLY
                     LKLLEEAIEDVKGIKKTEPKSYSIKVSKFVDDSYVSDDELKIMIHKEIGKIENKKDKD
                     KIISIFTDRFGELSNELLLYIESIYLNTLLLKYDIDRIVETKLFATVIFTKEKTKQLD
                     MEKVFMVAYNISNDFHFEYRQDQLLLKLSKEVQSKDWIYTFTSFFENLK"
BASE COUNT         2629 a         1482 c          986 g         3202 t
ORIGIN      
        1 ttgttttaaa attgcttctt gcatatattt aggatatgtt attttatcaa cttgagccct
       61 taatgctcta acggccgggc ctttactctt gttcaacatt ttaagttgaa ttaaacattt
      121 gtccactgct tttcccattt ctcctcctaa agcatctatt tccctaacaa cgataccttt
      181 agctggtcca ccaatagaag gattacatgc catacttgca atatgatcaa agtttcctgc
      241 gattaataaa gttttatgat ttcttctagc agtggccaat gccgcttcta ttcccgcatg
      301 acccccacct acaactacta cttcatacat atttttcacc ttttttaatt atatcacatt
      361 ttccttcttt tgtccttatc tattctcaaa aaaaatagag acatttaatc tgtctctatt
      421 ttgctatatc aacaacgttt attacaacgg tgtataatgg tttagtatag ctggatggtg
      481 taaatgtaat tttgaacttt ccttcaccaa gtgctaagaa atccgcaagt tttccaaaac
      541 cagctgaata taataatatt ttagcattat ttatatcaga accatctaaa gcttcgattt
      601 gccaactatc ttcagtaatt tcagcatcac caataccaaa ctctttatct gctttatcaa
      661 taatactact aaagcttatt gcctcataat attgatatgt ttgatcgcca aaagcatctt
      721 cttttaatcc ttcagaacta ttaatttcaa aatcttcgct gatgattttt aaggcttttt
      781 cgattggagt tatatactca tttaaagcgt tgtattgaga agatatctta aagtgtaaaa
      841 taccttcttt tacaatgcct aatttaaatg ttgttacacc tctagaagtt gttgttattc
      901 cgtctttaat tacaaatgcc atatcttttt ctactaattc aagttttaag tcttggtgcg
      961 tctcttctgg gaagatttga gcagttatat caacagtacc aataataaac tttctcttat
     1021 tgtctgcatt atcaatagct gttgagattg ctgaatcgct atagaattta taagataata
     1081 atctatcatc aatgtccatt aataattcta gtttaactgg ttttttcttt tcaagaatat
     1141 gaactgtaat tgtatgttta atattactat ctgcaagaga agataattct acctcaacaa
     1201 ttccatgata tgttgattta aatagtcctt tatcagaaat ggttgctaag ttagcttctg
     1261 taattgttct gcgcgtaatt ggatcttgaa taaatctttg tgaagaatca aaaacaggat
     1321 taacaacttt tttactccat gttacagttg cagctgcgtt ggcaggggca actaaagcac
     1381 caaattgaat ttcttgacca gcaataatag ttacactgtg ttctactgca acagttttac
     1441 cattaacatc tttttgtgta atttgtacgc tttcaactgg agcataagta taatctaatg
     1501 taataatgtt tgatttaatt tctacatcgc ccgccatagc aacggcttga agacggaacc
     1561 tttgttgtgt agcaagtgaa gtacaataaa cttcaccgtt ttgattaaga actaaaccta
     1621 cactagcagg gtctgcaata acttgattat catctgtacg gtgaagtaat ctccactcaa
     1681 ctttagcgct agcagttgca ggtgcaacca tacttcctag ttttataact tctcttaaat
     1741 aagcaatttt gttgtttgtt ggggtaactg ctgcagattt attttctaca acatcaataa
     1801 ctactgctgt tggatcgtga taattcacaa aaacttttaa aactgcataa ttgccatcaa
     1861 ctgttcttgc ataaatgaaa tgatttccac caaattgagg agtaaattta ccatttgcag
     1921 taatagaagc acgagactca tcgccaactt cccaagtaac ttcagtagtt tcgtttgtat
     1981 ttgaagttag agtatagcct aattgtaatt cttcgcctat ttccatttca ctattgaaac
     2041 cttcaggagt gctaatccta attcctgaag ttttaggact tgtaacttca ataattgctg
     2101 gtgcataaat atctctttct actagatgag ctttaatatt acctacgcct acagctaaag
     2161 cagtaatctt aactttatca ttggctattt tttcaaactt tataatattt ttactttctt
     2221 cggtaattgt atattcgatt tcactttcaa cattgaactg tcctgtaatt gatagaactt
     2281 ttgtttgccc tacctccata gtgactgagt gaacatcaaa tctaaatata tctgttgagc
     2341 cgtctttagc aatacatccg gccgtaaaaa acaaaacaaa gatagataaa aatataattg
     2401 cgattttctt tttcataatt tttctcctct attcatacta gtataccaca ataatgtctt
     2461 tttgttaaga aaaaaattct ttttgttctt tttccttttt gatatagata tcgaaaattc
     2521 ttaagttttc ttgatactct tttttggtta taaaacgata cctatttact cttcttattt
     2581 taataaaaca acttcgcttt cctaataaat gttgaaagaa aataacaaaa gaacctaaaa
     2641 ttaaaaaagc tccaaaaaca taaaaataaa ttttcgaatt ttgttctgat attattatag
     2701 aaggagcgat aagaaaaaat aaccccacca aaaagaaaaa aattaaagga gcaataacac
     2761 aataaaatgt tgccatctta acattcttta tatacgtttc ttttaaataa ctcggactta
     2821 tattggatgg tagatatctt aattctattt cttcttcatt aaaaacacga aaagaagcac
     2881 ataatgaata ccattctctt atcttataag atttaccacg ataagtgtat tttcctttat
     2941 aatatgtaac ttcttcttcg ttatttattt taattgtttt gtctttcttc atttttatca
     3001 ccttatatat tatacctatt taaaacaaaa aataaaaggc tatttttaat agcctttatt
     3061 tatcttattt tgctgcttta tcgatagaac caaatacttc cattctacca agcattgctc
     3121 ttttaattcc tgctgtacat ggtcctaaga tttttctagg gtcatatact gcatcatttt
     3181 taggatctgc aagtaaagct cttaagtctt ttgcaaattc ttgttgaagt tcagtgttaa
     3241 cattaatttt agctgtacct ctagcaatag cttttttaat ttgatcatct ggaatgccag
     3301 aaccaccatg taatactaaa ggaactttag ctgctttatt aatagcgtcc atttcgtcaa
     3361 accctagttt aggaacacct ttatatggtc cgtgagcact acctagtgca ggagctaaac
     3421 aatctactcc agcttctttt acaatacgta tacaatcttc taattttgcg tattgaactt
     3481 tacctacgat accatcttct tctccaccaa caattcctac ttcagcttct actgtaacac
     3541 cttgtgggtg tgcgtagtca actacttgtt tactaagtct aacgttttcc tcaattggat
     3601 gttttgaagc atcgatcata acagatgtga aaccagcatc aatagctgct ttacaagctt
     3661 caaatgttgt tccgtgatct aagtgcaccg ctacatctac tgtaatcttt tgatctttga
     3721 ttaatccttt aaccatcccc ataaccacat tgtagccgcc catatattta gctgcacctt
     3781 cagaaactcc taaaattaca ggtgaatttt tttcttgagc cactaataaa atagtttttg
     3841 cccattcaag gttattaatg ttgaattgac ctacagcata accttccttg tttgcttttt
     3901 taagcatttt cttcatatta actaaagcca tttgtacctc ctttgctctt tgttaattac
     3961 ttcttgaact tatctaaaat aaaataacca ataccaacaa catcacgacc aatatgcgct
     4021 cctactgctg gcgtaatata ttgtaaccca atttctgcgc gattgccata atattctttt
     4081 aaatctttaa caatttgttt acaagtttct atattaccgc tttggataac ttgaattaca
     4141 acatcttgtg aatctccgat ttccccaata actaattctt ttaatctttc aatcgcttta
     4201 gaataagttc ttactttatc aaaagtaaca atagcaccct cttcgtttaa ttccaaaatt
     4261 ggctttattg ctagccaact tcctaaagta ccgctaaaac gacctaatct accattcttt
     4321 acaagataat ctaaactgtc aaccacaaaa taagcgtgtg agttttgagt taaatactca
     4381 ctatactcta aaatttcttc aacgcttttt ccttctttaa ccatttcttt agctcttaaa
     4441 gcataatagc cttgtaaata cgtagctgtt ttagtgttta cagaatatag attaattctg
     4501 tctgcatact cttccttaag agtcataaac gtttggccaa tagaacttaa cttataagat
     4561 atcgaaaata aaattatatc tgtataacct tttttaatgt gatcttcaat cataatcatc
     4621 atatcaccta atgtaggtgc tgatgttgat ggaatgtctt ctttactttt tactttcttt
     4681 agcctttcat aaaattcatc tgctctaatt tcaataccat caactaaatg ttcttcccca
     4741 aaattaatta ctgaacgtaa agttgggatt tgtggatcat aaccgatgta atctaaacct
     4801 gcgtttacat cagtaataat tgcaatttta ctcattattc ctccttttgc tttttacttc
     4861 cactttggat attatatcat tatttaactg ttttttcaac ttcactcaat ttattttaca
     4921 atcattatac tatttgttat ttataaacat aagtataaag caaatagtga atgataattc
     4981 ctaaaactga aactcctgaa ataattaaag aagtagttaa atatttctta aactttaata
     5041 attgttttct ttttaaaata accattacta aagtaaggat tccaaaaaca attgctactg
     5101 catataaaat attaccaata ttttgaaaca aatctaaagg tatatagtct atatccttcc
     5161 ataaagcccg atattcagca ttaaataaaa ttaatcccaa agtaaccaca agaacaataa
     5221 atagtattat aatttttaat gctattataa tcgcgctaat tgctggcatt actgtcgcta
     5281 gaattgccgc tgcaagtcca ataaaaccta tgatttgtaa aaatcttatt aatttagact
     5341 tctgcatatg tttttttctc ctttgttatc atatattaca atacatttat tatacgaacc
     5401 ttaaactatt tcaaattttc aaagaaacta gtaaaagtat aaatccaatc tttagattga
     5461 acctctttac ttagctttaa taatagttga tcttgtctat attcaaaatg aaaatcattg
     5521 gagatattat aagcaaccat aaatactttt tccatatcta actgctttgt tttttcttta
     5581 gtaaagataa ctgttgcaaa taattttgtt tcaacaattc tatcaatatc atattttaat
     5641 aataacgtat ttaaataaat actttctata tataacaaca attcatttga tagttctcca
     5701 aatctatcag taaagataga gataatttta tctttatctt ttttattttc tatcttacca
     5761 atttctttat gaatcattat ctttaattca tcgtcagaaa cataactatc atctacaaac
     5821 ttagaaactt taatactata actcttaggt tctgttttct tgattccttt tacatcctca
     5881 attgcttctt ctagtaattt caaatataaa tctaaaccaa cactatcaat aaaccctgat
     5941 tgttctttac ccaaaagatc tcctgctcct cttatcgata agtctctaat tgcaatatta
     6001 aaaccactac ctaaggttgt gaattcttta atcgcctcta atctcttata tgctgtttca
     6061 gttaaaattt ttttattatc atacattaag aacgcataag caattctatc acttcgccca
     6121 actcttcctc taatttgata aatttgtgct aatcctaaat ggtcggcatc ttcaattatt
     6181 aaagtattgg cattaggaat atcaattcct gtttctataa ttgtagtgca aagtaaaata
     6241 tcatattcca aattaataaa tgaagctaca ttatcttcca atttttcttt gttcatttgc
     6301 ccatgagcaa taacaatttt tgcctctgga actaaacgct tcaaattaat tgctgtttta
     6361 tgcaaagtag atatctgatt gtgtaaataa aagatttgtc ccccgcgatt taactctcta
     6421 taaattgctt ctttaataat tgtgtcgttt ttagataaaa catatgtttg aattggatat
     6481 ctgctctttg gaggtgtttc aagcaaactt aaacttttaa taccagaaat agcaatatga
     6541 agtgttcttg gaatcggcgt ggctgttaaa gttaaaatat taatatcttt ttttaaagtt
     6601 tttattttct ctttatgcat tacgccaaat ctttgttctt cgtctactat caataaacct
     6661 aagtcataaa aaccaacatc atcagataaa atcctatgtg tcccaattaa gatgtctact
     6721 aaaccttttt tagtatcttc aataacttct ctttgctttc ttagtgggac taaacgattt
     6781 aaaagttcaa cccttattcc ataaggttct aatctatttt tgaacgtatt gtaatgttga
     6841 ttagagagaa tggttgttgg agcaagatag gcagcttgct tgttgttaat aactgtttta
     6901 acagcagttc ttaaagcaat ctcggttttt ccaaaaccaa catcaccaca aaccaacctg
     6961 tcaataactt taccatcttc catttcagct ttgatgtttt caataattgc agtttgatcc
     7021 ggagtttcct cgtgcgggaa gtcactttca ataatttttt gaaactcgtc atctggtaag
     7081 tatttataac cagttgcttt acttctttct gattcgttgt caataatgac agttgcaata
     7141 tcgactaatt gttccttaat tctttttcgt ttcttttccc aagatttagt tcctagttta
     7201 tttaattttg gaactactcc ttcactacca acatatttac tgattaaatt aatgttctca
     7261 acaggaacta aaagatcact attagaaaat tttaaagtta aatagtcttg ttttaaacca
     7321 tttaattcaa ctgttttaat accttgataa attgctaatc cataatcata atgaactata
     7381 taatcgccta atttaagttc atctttatca aaaattgttt ttccttgata agcaatagtt
     7441 tttttagttt tctttattga taaatcttta aaaatgtctt cttcagagat aacttctagt
     7501 ttttcgtcaa taaaaccaaa actaatagaa ttttctacta caatcaaatg aacctggttt
     7561 tcttctatat cttcaaagct ttcaatttcc aaataattca agctttcttc tataaccttc
     7621 ttaaacaatt ttaatctttc tgttgtttta aaagttaaaa tatatgtttt ttgtttattg
     7681 agttttattt caaaaattaa attttttaag tcattttgat aatcaattat tgaataagtt
     7741 ttaaaatcaa ttaatttatc caaagtaaca tctatatatt ttgaaaattg ttctaaataa
     7801 actttttgtt ttggtttatt aattgcttcg tattctaaag tatatagtaa ttcaatttgt
     7861 aattctaaac tttctctttg tgtttgaata tcaattaaaa tttttttata aacatttttc
     7921 aaatcgttgt attcatcata aaaagttaaa gagttttgat aatctaaaag acttgcttgt
     7981 tcatcataaa aataattaat taacttattt actctttcgt catttttgtg gttttctaca
     8041 tcttttaata attcttgaat gtgaggattg ttgtgttttt ttagtttaga aataacttct
     8101 tttttttgtt cttcattata aatcaattct tggataggat agatatctat ttcttctatt
     8161 ttctctaatg atagctgatc tttcacatca aaaaatctga tgctttcaat ttcgtcatca
     8221 aaaaaagtaa ttctaactgg cttttcatgc aaaataggga agatgtctat tacctcccca
     8281 cgcacgctaa aatcccccg
//
LOCUS       DAOSGS010000037         7326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_8800_length_7326_cov_4.354834, whole genome shotgun sequence.
ACCESSION   DAOSGS010000037 DAOSGS010000000
VERSION     DAOSGS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8800_length_7326_cov_4.354834"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLQ22_02415"
     CDS             <1..887
                     /locus_tag="PLQ22_02415"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOH58846.1"
                     /translation="IKTFGNIVCFRNLEKQVNDAIALFGNKDALGIVLLKSFKDYYEG
                     YDDFPGYVKLVERLTNEYPLGEEIIGEENERKFIKLFNTILKVRNILQSFDDFKDKEL
                     ISDYDYQDYQSIYIDLYDKYREQAKADAEDIDDDLEFELELVRSVEVNIDYILMLVSK
                     YYDDNRQDKEVVVKVTKAIDSSPSLRNKKDLILKFIDQVNVNEELDDEWKAYIKESKE
                     RELTKIIKEENLKYDATIKYVDKAFDTGEMKSIGTDITEILPATSMFKTKDGISREVK
                     KETVFQKLLAFFERFFGL"
     gene            965..1519
                     /locus_tag="PLQ22_02420"
     CDS             965..1519
                     /locus_tag="PLQ22_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH58847.1"
                     /translation="MNVEIDLSNVVLKTERLVLRPWKEEDLDDFYEYASVEGVGVMAG
                     WNRHTSIMESKTILDRFIAGKRTFAVEKEGKVIGSLGVEQYFERLFPELDSLKGRAIG
                     YILSKDYWGKGLMPEAVKEVIKYLFEEVKLDFIMITCYKDNPQSRRVIEKNGLSLYKE
                     IELPSASGKLKESYAFILRKENYK"
     gene            1595..2125
                     /locus_tag="PLQ22_02425"
     CDS             1595..2125
                     /locus_tag="PLQ22_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOH58848.1"
                     /translation="MKNLIVYGSKYGSSRKYAERLSELTGIYLADYKEVSDLKAYDNV
                     IYLGGIYASKVRGLKKTAKKLRNDARLILVTVSLGNPNLDDKLKEDVRTQNKKLIKKP
                     LLEKIKFFHLVGSMDYPNLKGFDKKIIKFVFKMFLKKDPQNQKIIDVYNGKVDELDYS
                     SLDKIVSYLEEKEIIK"
     gene            2246..2554
                     /locus_tag="PLQ22_02430"
     CDS             2246..2554
                     /locus_tag="PLQ22_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58849.1"
                     /translation="MSILESIKLAEKKAEDLRVNARRDLELKEEAVKAEIEKEVLKIE
                     KETKTKIDKSIEANDAEILKLNEDLKLKVKEDAKLITQEASKNKAKATTFILNEVLAR
                     "
     gene            2563..4437
                     /locus_tag="PLQ22_02435"
     CDS             2563..4437
                     /locus_tag="PLQ22_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOH58850.1"
                     /translation="MKKAKIVALKEEKEKLLLELQKSGLVMLIPTEEETILIDVSEND
                     DVIERTEKSLSFLKKYQEKKDSFLARTEVSYDEFFNYDQKKMDLLLELENLEKEIALK
                     EEENKALLEEIKLYEPWLKLEAKVEDLKSSRYTDLFVGYIGSKDEEKFLESLDALGSD
                     YKLYGLDKEKRAIIYASYYLDKEEVDLLNKEFQFSEHKFEDNGFVRDIVKNKEKQIIT
                     NNLFIDEASKKLAKLTEDKEKLYVLSDQAATDKELKLAPRQYTLETVYLEGWVRSDKE
                     DDLKKVLETSVSCYDLVLLDPEEGENPPTCNKNNKFVSPFQSVTNMYEVPNYKEVDPN
                     PMMSIWFWFIFGMMMGDVGYGVVMLIGGILFLKLKKPKGMLKQIGKIITYSSIPTILF
                     GVLYGSYFGFGWFEPVFVNPVKEPLTVLIFSFGVGLLHIVSGLVVKMYASFKAKDYWQ
                     AILDDFPWIVLLLGLGVFAIGLKIEGLSKIGLIMIAASASVITIFGGRRKKGFIRKIT
                     GGVTSLYGLVNHMSDVLSYSRLLALGLSTAIIAEVMNRLAGMVQGSIIGIIMSVFIYI
                     IGHIFNIGMGLLSAYVHDSRLQYIEFFGKFYEGGGYEFRPLSVQLKHVDAIKYKEEKI
                     "
     gene            4437..4898
                     /locus_tag="PLQ22_02440"
     CDS             4437..4898
                     /locus_tag="PLQ22_02440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOH58851.1"
                     /translation="MTLAIIGAALAAVLSGIGSAYGVLIGGRAAAGVVSEKPDLFGRL
                     LVLQALPGTQGIYGFIVAIMVLVQVGILGGTPVALTEAQGWLFFAACMPIAIVGLLSA
                     IFQAKMSVAAIHMTAKQPDASGKGMTMTVLVETYAILALLVSILMVIGIPV"
     gene            4910..5533
                     /locus_tag="PLQ22_02445"
     CDS             4910..5533
                     /locus_tag="PLQ22_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOH58852.1"
                     /translation="MAKTIYDKIIDKGKEDAKVILADGLKKLEQLKLEQIEEAKNKQK
                     DELKKQEALNHSKKQAALINARREAKKSEIKLKQALLKETVKEAVAKLDNLKQNELFD
                     FVLKLVKEDDLSGKEVIYVNKKDYSKYLATFSSKKEDELVELDKLNNALGKKFELKLS
                     KKPKDLDGGFYIVSEKYDINHSYSALVDNLLAKLEADLNKILFSEVK"
     gene            5533..6483
                     /locus_tag="PLQ22_02450"
     CDS             5533..6483
                     /locus_tag="PLQ22_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOH58853.1"
                     /translation="MNYPYANGVLKVKEADLFTNASYLRLKDSKDILKTLEELGYEKK
                     QTIEATLVNELVKTKDLIQSLSPNHKLTNCFFLVSDATNIKAYYKARIFNLSTDDLYV
                     PNSNFSKEELEEAILNYDLKGLSKENQKLFIQLEQELKGLEENPRLISAKIDQILFNK
                     AYKDSKKINDKALREYLVLNIDTINIITLFRAKKLNFDLSEVLDVFIDNGKIKIDVLK
                     DLYQEENEDRILKEIVLVYPKLEKPLLSYFKNQSLDLLERAFKSYILEEIAKYEYDSM
                     KLGPMLYYYVSKEIEIENIRLIYFDQSIALSDLIKGDRNG"
     gene            6476..6790
                     /locus_tag="PLQ22_02455"
     CDS             6476..6790
                     /locus_tag="PLQ22_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOH58854.1"
                     /translation="MAKVDIVAIAEDDTVLLFNAIGIKTYTIKERSVLEKKIAELAIE
                     KCKIIYLTEKLYELIPEVIEKYELESYPIIIPLPLKETKESVGVKQIKKNVEKAIGFD
                     IL"
     gene            6803..>7326
                     /locus_tag="PLQ22_02460"
     CDS             6803..>7326
                     /locus_tag="PLQ22_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOH58855.1"
                     /translation="MKDKIIGIITKVSGPLIVAKNMQDVQMYDVVKVSEKGLIGEVIE
                     LRGDRASIQVYEETAGIGPGEPVYSTGEALSVELGPGLMESIFDGIQRPLAVIYESSG
                     DRIPLGIDLPRLDREKIWEFKAVAKKGDKVEAGDVLGTVQETSVVEHKIMVPFGVKGE
                     VLEVKSGNFKVTDVV"
BASE COUNT         2860 a          745 c         1376 g         2345 t
ORIGIN      
        1 caattaaaac atttggtaac attgtttgtt ttagaaactt agaaaaacaa gtaaatgatg
       61 ctattgcttt atttggaaac aaagatgctt taggtatagt tttactaaaa tcatttaaag
      121 attactatga aggctatgat gatttccctg gatatgtgaa attggtagaa agattgacta
      181 atgaatatcc tttgggtgaa gaaattattg gtgaagaaaa cgaaagaaaa tttattaaat
      241 tatttaatac tattttaaag gttagaaata tcttgcaatc ttttgatgat tttaaagata
      301 aagaattaat tagtgactat gattatcaag attatcaaag catctatatc gatttatatg
      361 ataaatatcg tgagcaagca aaagctgatg ccgaagatat tgatgatgat ttagagtttg
      421 aattagaact tgttagatca gtagaagtta atatcgatta tattttaatg ttagtttcta
      481 agtactatga tgacaacaga caagataaag aagttgttgt taaagttacc aaagctattg
      541 actcatctcc atctctaaga aacaagaaag acttaatctt aaagtttatt gatcaagtta
      601 atgtcaatga ggaacttgat gatgaatgga aagcatatat taaagaatca aaagaacgag
      661 aacttacgaa aattataaaa gaagaaaact taaaatatga tgcaacaatt aaatatgtag
      721 ataaggcatt tgatactggt gaaatgaaat ctatcggtac tgatattact gaaatattac
      781 cagcgacatc aatgtttaaa acaaaagacg gaataagtag agaagttaaa aaagaaacag
      841 tatttcaaaa actattagca ttttttgaaa gattctttgg tttataaaaa agggcttttg
      901 ccctttttta aattgtaatt agaatataga tttggtataa ttatattatt ataagaggta
      961 aattatgaac gtagagatag atttaagcaa tgttgtttta aagactgaaa gattagtttt
     1021 aaggccttgg aaagaggaag atttagatga cttttatgag tatgcaagtg tagaaggtgt
     1081 tggtgttatg gctggctgga atagacatac tagtattatg gaatcaaaaa caattttgga
     1141 tcgttttatt gctggtaaaa gaactttcgc tgtagaaaaa gaggggaagg ttattggttc
     1201 attaggtgtt gaacaatatt ttgaaagact atttcctgaa cttgactctt taaagggtag
     1261 agcaattggc tatattttgt cgaaagacta ttggggaaag ggcttaatgc cggaagcagt
     1321 aaaggaagtt attaaatact tgtttgaaga agtaaagcta gactttatta tgattacatg
     1381 ttataaagat aatcctcaat cacgaagggt aattgagaaa aacggattga gcttatataa
     1441 agaaattgaa ttacctagtg ctagtggcaa actaaaagag agttatgctt ttattttaag
     1501 aaaagaaaat tataaataaa agttctgtaa atagttagta aagtaaagag ttttcctttt
     1561 cttttatgat agaataaaaa tataggagaa gcttatgaag aatttaattg tctatggtag
     1621 taaatacgga tctagtagga aatacgctga aaggttaagt gaattaacag gaatttatct
     1681 tgcagactat aaggaagttt ctgatttaaa agcatatgat aatgttattt atttaggtgg
     1741 tatttatgct agtaaagtta gaggcttaaa gaaaacagct aagaagttaa gaaatgatgc
     1801 tagattaata ttagttacag tttctttagg aaatcctaat ttagatgata aacttaaaga
     1861 agatgtaaga acgcaaaaca agaaattaat taaaaagccc ttgttagaaa aaattaaatt
     1921 ctttcattta gtcggtagta tggactatcc aaacttaaaa ggatttgata agaaaattat
     1981 taaatttgtt tttaaaatgt ttttaaagaa agatccccaa aatcaaaaaa tcattgatgt
     2041 ttataatggt aaagttgatg aattagacta tagcagttta gataaaatag ttagttattt
     2101 agaagaaaaa gagattatta aatagagaaa ataagccttt gaaaaaggct tatttttaat
     2161 agattagggt attatttctt tcttgtagat ttaagtttaa tatgatataa tatatcgatt
     2221 gaattaatga aaaggtggtg atgagatgtc aattttagaa tcaattaagc tggcggaaaa
     2281 gaaagctgaa gatttaagag ttaatgctag acgtgattta gaattaaaag aagaagcagt
     2341 taaggcagag attgaaaaag aagttttaaa gattgaaaaa gaaactaaaa caaaaatcga
     2401 taaatcaatt gaagctaatg atgctgagat tttaaaacta aatgaagatc taaaattaaa
     2461 agttaaagaa gatgctaaac ttattaccca agaggcttct aaaaacaaag ctaaagcgac
     2521 taccttcatt ttaaatgagg tgcttgctag atgattgttt caatgaagaa agctaaaatt
     2581 gtagctttaa aagaagaaaa ggaaaagctt cttttggaat tacaaaagtc aggattagtg
     2641 atgctaatcc caactgaaga agaaactatt ttaattgatg tttcagaaaa tgatgatgta
     2701 atagaaagaa ctgagaaaag tttatctttc ttaaaaaagt atcaagaaaa gaaagattca
     2761 ttcttagcaa gaacagaagt aagttatgat gaattcttta attatgatca aaagaagatg
     2821 gacttacttt tagaactaga gaatttagaa aaagaaatag ctttaaagga agaagaaaat
     2881 aaagctttac ttgaagaaat aaagctttat gagccttggt taaagttaga agcaaaagtg
     2941 gaagatttaa agtcatcaag atatactgac ctttttgtag ggtatattgg tagtaaggat
     3001 gaagaaaagt ttcttgaaag tttagatgct ttaggctctg attataaact atatggtcta
     3061 gataaagaaa aaagagcaat tatttatgct tcatattatt tagacaaaga agaagttgac
     3121 ttactaaaca aagaatttca attttctgaa cacaagtttg aagataatgg gtttgtaaga
     3181 gatattgtta agaataaaga aaagcagatt attactaata acttatttat tgatgaagct
     3241 agtaaaaagt tagctaaact aacagaagat aaagaaaaac tttatgtttt aagtgatcaa
     3301 gctgcaactg acaaagaatt aaaacttgct ccaagacagt atactttaga gacagtttat
     3361 ttagaaggct gggtaagaag tgataaagaa gatgatttaa agaaagtttt agaaacttca
     3421 gttagttgtt atgatttagt attacttgat ccagaagaag gagagaatcc acctacatgc
     3481 aataaaaaca ataagtttgt atcaccattt caaagtgtta caaacatgta tgaagtacct
     3541 aattataaag aagtagatcc taacccaatg atgagtattt ggttttggtt tatttttgga
     3601 atgatgatgg gtgatgttgg ttatggtgta gtaatgctta ttgggggaat tttattctta
     3661 aagcttaaaa aacctaaggg aatgttaaag cagattggta agattattac ttactcatct
     3721 atcccaacaa tcctttttgg agttttgtat ggatcttact ttggttttgg ctggtttgaa
     3781 cccgtttttg taaacccagt taaagaacca ttaaccgtat taatcttttc ttttggtgtt
     3841 ggtttacttc acattgtctc tggtttagtt gtaaagatgt atgctagctt taaagctaaa
     3901 gattactggc aagctattct agatgacttc ccatggatag tattattact tggacttggt
     3961 gtttttgcaa ttggtcttaa aatcgaaggt ttatcaaaaa ttggtttgat tatgattgct
     4021 gcatcagctt cagtaattac aatctttggt ggaagaagaa agaaaggttt tatccgtaaa
     4081 attacaggtg gggttacgag cctttatgga cttgtaaacc atatgagtga tgttttaagt
     4141 tattcaagat tacttgcttt aggactatcg actgcaatta ttgctgaggt tatgaataga
     4201 ctagcaggaa tggtacaagg aagcattatt ggtataataa tgtctgtctt tatttatata
     4261 attggtcata tctttaatat aggtatggga ttactatctg cttatgttca cgatagcaga
     4321 ttacaatata ttgaattttt tgggaaattt tatgaaggtg gaggatatga atttagacca
     4381 ctttcagttc agttaaagca cgttgatgca ataaaatata aggaggagaa aatataatga
     4441 cattagcgat tattggtgca gcattagctg cagtattaag tggaattggt tctgcttatg
     4501 gagtattaat aggcggaaga gcagcagcag gagttgtttc agaaaaacct gatttatttg
     4561 gacgtttact tgtattacaa gcattacctg gaacacaagg aatttacgga tttatcgtag
     4621 caatcatggt actagttcaa gtaggaatat taggaggaac tccagtagca cttactgaag
     4681 ctcaaggctg gttatttttt gcagcttgta tgccaattgc aattgtaggt ttattatcag
     4741 ctattttcca agcaaagatg tctgtagcag ctattcatat gacagctaaa caaccagatg
     4801 cttctggtaa aggtatgact atgacagtat tagttgagac atatgcaatt ctagctttac
     4861 ttgttagtat tttaatggtt attggtattc cagtatagga aataaaataa tggctaaaac
     4921 aatttacgat aaaattatcg ataaaggaaa agaagatgct aaggtaatct tagcagatgg
     4981 cctaaaaaaa ctagaacaat taaaattaga acaaatagaa gaagctaaaa acaaacaaaa
     5041 agacgaatta aaaaaacaag aagcgttaaa tcatagtaaa aaacaagcag ctttgattaa
     5101 tgcaagacgt gaagctaaaa agtcagaaat taaattaaaa caagctttat taaaagaaac
     5161 agtaaaagaa gcagtagcta aattagataa cttaaaacaa aatgaacttt ttgatttcgt
     5221 tttaaaatta gtaaaagaag atgatttatc aggtaaagaa gttatctatg taaataagaa
     5281 agactattct aaatatctag ctacattttc ttcaaaaaaa gaagatgagt tagttgaatt
     5341 agataaacta aataatgctt taggtaaaaa gtttgaactt aaattatcta agaagccaaa
     5401 agatttagat ggtggttttt atatagtttc cgaaaagtat gatattaatc attcatactc
     5461 tgctttagta gataaccttt tagcaaaatt agaagcagac ttaaacaaaa ttttattcag
     5521 cgaggttaag taatgaatta tccttatgcg aatggagttt tgaaagtaaa agaagcagat
     5581 ctatttacta atgcttcata cttaaggtta aaagactcaa aagacatttt gaagacttta
     5641 gaagaattag gctatgagaa aaaacaaaca atcgaagcaa ctttagtaaa tgagttagtt
     5701 aaaactaaag atttgattca aagtttaagt cctaatcata aacttacaaa ttgtttcttt
     5761 ttagtaagtg atgctactaa tataaaagca tattacaaag caaggatttt taatttaagt
     5821 acagatgatt tatatgttcc aaatagcaac tttagtaaag aagaattaga agaagctatt
     5881 ttaaactatg atctaaaagg tttatcaaaa gaaaatcaaa aactatttat tcaactagaa
     5941 caagagttaa aaggattaga agaaaatcca aggttaatta gtgcgaaaat agaccagatt
     6001 ttatttaata aagcttataa agatagcaag aaaataaatg ataaagcttt aagagaatat
     6061 cttgttttaa atattgatac gataaatata attactttgt ttagagctaa aaaacttaat
     6121 tttgatttaa gtgaagtttt agatgttttt atcgataatg gaaaaataaa aatagatgtt
     6181 ttaaaagact tatatcaaga agaaaatgaa gatagaattt taaaggaaat agtattagtt
     6241 tatcctaaac tagaaaaacc tttactaagt tactttaaaa atcaatcttt agatttactt
     6301 gagcgagctt ttaaaagtta tatactagaa gagattgcga aatacgaata tgattcgatg
     6361 aaacttggac caatgcttta ctattatgta agtaaagaaa ttgaaataga gaatattaga
     6421 ttaatttatt ttgatcaaag tattgcttta agtgatttaa ttaaaggaga tagaaatggc
     6481 taaagtagat attgtagcga tagcagaaga tgatacagtt ttacttttta atgcaattgg
     6541 cattaaaact tatacaatta aagaaagatc agttttagag aagaaaatag ctgagcttgc
     6601 aatagaaaag tgtaagatta tctatttaac tgaaaagtta tatgagctta ttccagaagt
     6661 tattgagaag tatgaacttg aaagttatcc aattattatc cctcttcctt taaaagaaac
     6721 taaggaaagt gttggtgtca agcaaatcaa gaaaaacgta gaaaaagcaa ttggctttga
     6781 tatcttatga ggagactaac gtatgaaaga taaaattata ggaataatta ccaaagtttc
     6841 aggaccctta attgtagcta agaacatgca agatgttcag atgtatgatg tagttaaggt
     6901 aagtgaaaaa ggattaattg gtgaagttat agaattaaga ggagatcgtg cctcaatcca
     6961 agtttatgaa gaaacagcag gaattggacc aggagaacca gtatactcta caggtgaggc
     7021 tttatcggtt gaacttgggc ctggactaat ggaaagtatc tttgatggta ttcaaagacc
     7081 actagctgtt atttatgaat catctggaga tagaattcct ttaggtattg acttaccccg
     7141 tttagatagg gaaaagattt gggaatttaa agctgtagct aaaaaaggtg ataaagttga
     7201 agcaggtgat gtactaggta cagtccaaga aacatcggtt gtagagcata agattatggt
     7261 accttttggt gttaaaggtg aagttttaga agtaaaatca ggaaacttta aagttactga
     7321 tgtagt
//
LOCUS       DAOSGS010000038         6437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_10536_length_6437_cov_4.177060, whole genome shotgun sequence.
ACCESSION   DAOSGS010000038 DAOSGS010000000
VERSION     DAOSGS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10536_length_6437_cov_4.177060"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..299)
                     /locus_tag="PLQ22_02465"
     CDS             complement(174..299)
                     /locus_tag="PLQ22_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58856.1"
                     /translation="MKFLEKTYMGFPNNMSYDWDLIAESSKVYINNCGDPFSTLS"
     gene            complement(968..2170)
                     /locus_tag="PLQ22_02470"
     CDS             complement(968..2170)
                     /locus_tag="PLQ22_02470"
                     /inference="COORDINATES: protein motif:HMM:NF022887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3201 domain-containing protein"
                     /protein_id="HOH58857.1"
                     /translation="MIKPKFTFEKIKFSIDPPTFKKAIDLYETSKIKNFEETAYCYTA
                     KVQGTSLYKVTVDIDHYDYGDCNCYIGKQGDLCKHMIAVAIYALFRGEKIPEDNKEIL
                     LHPVCSNKKGELNESELVEVKEKISQAVKLIRAYSGPSSTWFHYQNNLSEGVNRLTEV
                     VSKLPVSYQTADILVRLLVRLDKKLVSGGVDDSDGTVGGFIQDVVEILKDFAKLETEC
                     LKAFNKLKGISTCFGWEDSLLQLILPSDVFYITKIHDELKMYDYQVGDIEYTSGNYNE
                     TGKLIKDKIPTITSEYGEIGLIDGKLYFVMIFEKEILSEKTLEDIAKIRKYKLYGFND
                     FLNDIDIKTVFSKSCKEKYIQIQYDNIIETDYYEIIRIHDKLKNMFLENKIYPINQLN
                     INLRSETV"
     gene            complement(2412..3842)
                     /locus_tag="PLQ22_02475"
     CDS             complement(2412..3842)
                     /locus_tag="PLQ22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOH58858.1"
                     /translation="MNDLIKQILEIPEENQVIEFKRLNGNKVIAKIIQTIVSMTNTEG
                     GTIIIGVDDPEKTTLKGVDRIFGIEENLELFDSISEEIQKIVPPLSDICQPKKIHIDE
                     INKTVALLYIPKATDNFRSINRKVYIRQYKSNIELTPQEVIKFAYAKGFEKADKELVD
                     IDMELLNTETFGLWKKARKLPDEEIEKLLFKTGLARKDDKGIIKPTRAAVLLFAEYPN
                     DLMETKCTIRIFQYEGTIETVKETLNLIGTPKTIGGPILKQINDAHEYVLTLLRSGIK
                     VPSGFKTEYLIPERAVKEAITNAVIHRDYFIKRDIEVRIFEDRVEIQSPGLFPYNITK
                     YNIGLVRADGYRNDLIVKHLREFPEPPNLDQSEGIRAMRSEMKGQNLYPPIFLTYPLL
                     QDSVRVVLINEKVATEWEKVNYYLEKNKYITNEEARNLTRVEQRDKMTKILKNWVEKG
                     LLVQIVPQTGYVKGTKYRLPNGSEIS"
     gene            complement(3878..>6437)
                     /locus_tag="PLQ22_02480"
     CDS             complement(3878..>6437)
                     /locus_tag="PLQ22_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH58859.1"
                     /translation="TGTGKTVLMASLILYYYKKGYRHFIFFVNQNNIVDKTENNFINK
                     NHNKYLFKQNIVIDDKTVNIKKVESFDDSDDIQIKFTSIHKLHNAVYLAKENAVTLED
                     LQKRNLVMIGDEAHHFNASTMKNGQMNLDMPTELKDNSSASDVERSWENTVLNKILKK
                     EGYSGQSENRNVLLEFTATIPNNQEVKEKYRTKTIYKYELIDFLNAGYTKEINLISTS
                     LNKKEKILLALLFNWYRHKIALLNNIPNFKPVILFRSKYIEESKNDYREFLTTVKELQ
                     KTDFEFLSSIEKKLQSEQRSVLKVYEQGASRIKQMIGFINEQNIDFNDIISFIKADFD
                     ERNCIITNSKDNKTTTKEKTTEEQEQLLNSLEDKNNHIRAIFTVKRLTEGWDVLNLYD
                     IVRLYEGRDEGRDKLGKVKAGQTTTQEIQLIGRGVRYYPFEYKETEASKRKFDEDLEN
                     PLRILEEFYFHSENDIRYLDELKRELKNKGFIQDNRIVKKYKLKEDFENTDFFKEVKV
                     WKNERIDNPEKRKLNLSEIKTDPQFNPFTYEIKGFSLKEQKVDLNEKFDNVLAESKPA
                     YKRSIQISFKEIDKHIIRKAINIKAKADNSILKYENLTNELNISSIDDIFNDKYLGDM
                     KFNIITESETVNLSDISADDLLNCLLIFFEQFTSKLKEINNPYKGDTKFKHFSFKELF
                     GDEKEKSVLINEESKLLEDEMLKYKWYVLNGFNGTSEERELLEFLKNRIVNLQEKYDE
                     VYLLRNEEVYKIYDFVKGRGFEPDFLLFLKGKNKTLYYQIFIEPKGDEFKDKSGLFTE
                     SKEGWKEEFMKQISHKYGNGEIWKTESRKYVLYGLPLFNSKDKVSFENEFNKFI"
BASE COUNT         1932 a         1118 c          804 g         2583 t
ORIGIN      
        1 tcggaaatat ataatacccc atcaggataa atttccctcg caataaataa tccttccata
       61 tttccctctg ttccaccagc agtcatatat ccccacccta atgcgacatc aagcttatat
      121 aagcttaaaa aatatttaac aacttcttgc tcgaaaggct ttgaatgttt tttctatgaa
      181 agagtcgaaa acggatcccc acaattatta atatatactt tagaagattc agcaattaaa
      241 tcccaatcat atgacatatt attaggaaat cccatatatg ttttttcaag gaatttcata
      301 ttatataaat aatccttgat ctcttgctct aaaattgtca tattcattca ataatattaa
      361 ttttatgaca gataatattt cggaagactt attaaaaagc tataaatttt caaatagatg
      421 tataataaat atatgtaatg gtacaattgt atcatatact atgagccctg tcaatggttg
      481 gataactgtt ggaataaagt attagtttca agaagaatgt gtgagaaaat gaccaatagt
      541 atttccagaa acctcaagtg atgcacttag ttttaaattc cgtcatttta tacgcagtat
      601 actaaaatac aaaaatacca acgatatcat cacacaattg agaatgcttt ataattttgg
      661 ttctcaaata tcaagatatt gggaaatagc ctcatttgac cagcgtagag agatactggc
      721 ggacatgatt acgaactgtc caaagattaa aaatgggaaa cttacgaact tagaaatcag
      781 cccattgtat aaggttttta gtgatcttgc aaaagcactc atcctgaaaa atcggggaga
      841 ccaccgctga gcggggtatc tagctgttcc tattcattac actcatagac atcggagttg
      901 aaagacgtac ttacgaattt caactttata gagattgtag ataattttaa tttatattgc
      961 aaggttttta gacagtttcg gatcttaaat ttatattcag ttgattaatt ggataaatct
     1021 tattttctag aaacatattt tttaacttat cgtgaatcct tataatttca taataatctg
     1081 tttcgattat attatcatat tgtatttgaa tgtatttttc tttacagctt tttgaaaata
     1141 ctgttttaat atcaatatca tttaaaaaat cattaaatcc atacaattta tattttctta
     1201 ttttggctat gtcttcaaga gttttttctg ataatatctc tttttcaaaa atcattacaa
     1261 aataaagttt accatcaatt aatcctattt ccccatattc tgatgtgatc gttgggattt
     1321 tatctttaat aagtttgcct gtttcgttat aatttccaga tgtgtactca atgtctccaa
     1381 cttgatagtc atacatctta agctcatcat gtattttggt aatatagaat acgtcacttg
     1441 gcaatattag ttgcaataaa gaatcttccc agccaaaaca ggtactgatt cctttaagct
     1501 tattaaatgc tttcaaacat tctgtttcta gttttgcgaa atcttttaag atttcaacaa
     1561 catcttgaat aaaacctccg actgtaccat cagaatcatc aaccccacca cttacaagct
     1621 ttttatcaag tcttacaaga agtcttacta aaatatcagc agtttgatag cttacaggca
     1681 gttttgaaac tacttcggtt agtctattca caccttctga taaattgttt tgataatgaa
     1741 accaggtact tgatgggcct gagtaagctc ttattaattt tactgcctgt gaaattttct
     1801 ctttaacctc tactagttca ctttcattta attctccttt tttattactg caaacaggat
     1861 gtaacaaaat ttctttatta tcttctggga ttttttctcc tctaaacagt gcataaattg
     1921 caactgctat catatgttta cataaatcgc cctgtttccc aatataacag ttacaatctc
     1981 cataatcata atgatctata tctactgtta ctttatataa acttgttcct tgaacctttg
     2041 cagtgtagca ataagctgtt tcttcaaaat tttttatttt actagtctcg tatagatcta
     2101 ttgctttttt aaacgtagga ggatctattg agaacttaat tttttcaaat gtaaattttg
     2161 gttttatcat aatttaagtt tttaatttag tagtcagtga atatgtattt aaccgccctc
     2221 tagctttgtg gcggtcggtc tattgcatat cactagcttt cagttatttt taaaaatata
     2281 aacattagct ctcatcactt atgtaaaaat tatcatcata aacttgtaaa aaataataaa
     2341 actcccattg taacagtgtt tttgttttac aagtttttgc agattctcat aaaacatgaa
     2401 attggtcatt actaactaat ttctgaacca ttgggtaacc tgtattttgt tccctttaca
     2461 taaccagtct gaggaactat ttgaacaagt aacccttttt ctacccaatt tttcagtatt
     2521 tttgtcattt tatctctttg ttcaaccctc gtaagatttc tagcctcttc atttgtaata
     2581 tatttatttt tttctaaata ataatttact ttctcccatt cagttgcaac tttttcatta
     2641 attaaaacaa ctcttactga gtcctgcaaa agaggataag ttaaaaaaat tggtggatag
     2701 agattctgtc ccttcatttc tgacctcatt gcacgaattc cttctgattg atctaaatta
     2761 ggaggttctg gaaattctct taggtgtttt acaattaaat cgtttctata tccatctgct
     2821 cttacaagcc caatattgta ctttgtgata ttatatggaa ataatcctgg actttgaatc
     2881 tcgactctat cttcaaagat tcttacttct atatcacgtt taataaaata atctctatga
     2941 ataactgcat ttgtaatggc ctcttttaca gctctttccg gtattaaata ctccgtttta
     3001 aatccagatg ggactttgat tcctgacctt aataaagtaa gaacatattc atgtgcatca
     3061 tttatctgct ttaaaatcgg acctccaatt gtctttggag taccaataag attaagagtt
     3121 tctttcactg tctctattgt accttcgtat tgaaaaattc ttattgtaca cttagtttcc
     3181 attaaatcgt tcgggtattc tgcaaataaa agaactgcag cacgtgtagg tttaataata
     3241 cctttatcat ctttacgagc taatccagtt ttaaataata atttttcgat ttcttcatcg
     3301 ggaagcttac gagccttttt ccacaatcca aacgtttcag tattaagcaa ttccatatct
     3361 atatctacta gttccttatc agctttttca aaacctttgg cataagcaaa tttaattact
     3421 tcttgcggtg ttaattcgat attactcttg tattgcctaa tataaacttt tctgtttatt
     3481 gaccgaaaat tgtctgtcgc tttgggtatg tataataaag caactgtttt atttatttca
     3541 tcaatatgaa tcttctttgg ttgacatata tctgaaagag gaggaacaat cttctgaatc
     3601 tcttcgctaa tagaatcaaa cagttctaaa ttttcctcaa taccaaatat tctatcaaca
     3661 cctttcaacg ttgtcttttc tgggtcatca actcctatta ttattgtgcc accttctgta
     3721 tttgtcattg agacaattgt ttgaattatt ttcgcaatga ctttatttcc gttcaatctt
     3781 ttaaattcga ttacttgatt ttcctcaggt atttctaata tttgctttat taaatcattc
     3841 attctattta ttggttagtt taaaataatt atcttactta aataaattta ttaaattcat
     3901 tttcaaaact tactttatct ttactattaa agagaggtaa accatataaa acatattttc
     3961 tgctctcggt tttccaaatc tctccattac catatttatg agatatctgc ttcataaatt
     4021 cttctttcca cccttcctta ctttcagtga ataatcctga tttatccttg aattcatctc
     4081 ctttaggttc aatgaaaatt tgataataaa gtgttttatt ctttcctttt aggaacaata
     4141 ggaaatcagg ctcgaatcct ctccctttta caaaatcata tattttgtat acttcttcat
     4201 ttctaagtag atacacttca tcatattttt cttgcaaatt aacgattcga tttttcaaga
     4261 attcaagaag ctctctttcc tcgcttgttc cattaaaacc attcaacaca taccatttat
     4321 atttcaacat ttcatcttca agtaacttac tctcttcatt aattaaaaca ctcttttctt
     4381 tttcatctcc gaataattct ttaaatgaga agtgtttaaa ctttgtatca cctttatatg
     4441 gattattaat ctctttaagt ttgcttgtga attgctcaaa gaaaatcagt aagcaattta
     4501 acaaatcatc tgcacttata tctgataaat ttacggtttc gctttctgta atgatattga
     4561 atttcatatc tccaagatat ttatcattga atatatcatc aatcgaggag atgtttaatt
     4621 cattggttaa attttcatat ttcagaattg aattatctgc ttttgccttg atatttattg
     4681 cttttctaat tatgtgttta tctatctctt tgaaagatat ctgtatcgat cttttgtatg
     4741 ctggttttga ctctgccaat acattatcaa acttttcatt aaggtcaact ttctgttctt
     4801 ttagagaaaa tcctttaatt tcataagtaa acgggttaaa ttgtggatct gtttttattt
     4861 cagataaatt gagctttctt ttttcaggat tatcaattct ttcatttttc caaactttta
     4921 cttctttgaa aaagtccgtg ttttcaaaat cctcctttaa tttatacttt ttaacaatcc
     4981 tattatcctg aataaatcct ttattcttta attcacgttt taattcatcc agatatctaa
     5041 tatcattttc gctatgaaaa taaaattctt ctaatattct taacggattt tctaagtcct
     5101 catcaaactt ccttttactt gcttctgttt ctttgtattc aaaaggataa tatcttacac
     5161 ctctcccgat aagttggatc tcttgtgtag tcgtttgtcc tgctttaact ttacccaatt
     5221 tatctcttcc ctcatcacgt ccttcataca aacggactat gtcatacaaa ttcaatacgt
     5281 cccaaccctc tgtaagtctc ttaactgtaa atatggctct tatgtgatta tttttgtctt
     5341 caagactgtt taataactgt tcttgttctt ctgtagtttt ttcctttgtt gtagtcttat
     5401 tatcctttga atttgtaata atgcaatttc tttcatcaaa atcagctttt ataaaactga
     5461 taatgtcgtt aaagtcaata ttctgctcat taataaatcc aatcatttgt tttatcctgc
     5521 ttgcaccttg ctcatatact tttagaactg acctttgttc tgattgcagt ttcttttcaa
     5581 tgcttgataa aaattcaaaa tcagtttttt gtaattcttt tactgttgtt aaaaattcac
     5641 gatagtcatt ttttgattct tcaatgtatt tgcttctaaa taaaataact ggtttgaaat
     5701 tgggaatgtt atttaataaa gctatcttgt gtctatacca attaaagagt agtgctaaca
     5761 ggattttttc ttttttattt aaagaagtgg aaattaaatt tatctctttt gtgtatcctg
     5821 cattaagaaa gtctattaat tcgtatttat atattgtttt tgtgcggtat ttctctttta
     5881 cttcctgatt gtttggaata gtcgcagtaa attcaagcaa aacatttctg ttctcacttt
     5941 gtcctgaata accttctttt ttaagtattt tattcaaaac agtattttcc cagcttcttt
     6001 caacatcact ggcacttgaa ttatctttaa gctcagtcgg catatctaaa ttcatttgtc
     6061 cgtttttcat cgttgaggcg ttaaagtggt gagcttcatc accaatcatc acaagattcc
     6121 ttttctgcag atcttcaagt gttacagcgt tttcttttgc cagataaact gcattatgta
     6181 acttgtgaat ggaagtaaat ttgatctgaa tatcatcact atcatcaaaa ctttcgactt
     6241 tcttgatatt tactgtttta tcgtcaatta cgatgttttg tttgaataag tatttattat
     6301 ggtttttatt gataaagttg ttttcagttt tatctacaat attattttga tttacaaaga
     6361 agatgaagtg gcgataacct tttttgtagt aataaagaat cagtgaagcc atgagtaccg
     6421 ttttacctgt tccggtt
//
LOCUS       DAOSGS010000039         6359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_10743_length_6359_cov_4.127855, whole genome shotgun sequence.
ACCESSION   DAOSGS010000039 DAOSGS010000000
VERSION     DAOSGS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10743_length_6359_cov_4.127855"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLQ22_02485"
     CDS             complement(<1..422)
                     /locus_tag="PLQ22_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58860.1"
                     /translation="MSAIKLSSRNLISKLRRTVLVCIAGSIGIIGIASVLSLSQGVTN
                     YVDDMQNDMLTGNPITIDETGINIASFMETMSMREKQDALQYAYKDGHINIFALLENL
                     VKRQEQLTSFGIKNEIDEVYQTFIKEMPEDKYGAYVFD"
     gene            complement(462..1160)
                     /locus_tag="PLQ22_02490"
     CDS             complement(462..1160)
                     /locus_tag="PLQ22_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58861.1"
                     /translation="MLRLENITKDYKVSWGKVHALRGINLSFRKAEFVSILGPSGCGK
                     TTLLNIIGGLDHYTSGNLYIDGIPTDKFTDRDFDTYRNHRIGFVFQSYNLIPHQTVLE
                     NVELSLNIAGVDKETRRKKAIEVLDKVGLQGEYNKRPNQLSGGQQQRVSIARALINNP
                     EILLADEPTGALDTETSKQIMELMKEISKERLVIMVTHNSKIAYEYSTRIIKLEDGLV
                     ISDSNPHEPAELII"
     gene            complement(1297..2562)
                     /locus_tag="PLQ22_02495"
     CDS             complement(1297..2562)
                     /locus_tag="PLQ22_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HOH58862.1"
                     /translation="MSKIKKVKILSSHELELLEDCSKGDVIDLKNLDQIQLDLGIIEK
                     EIKEGKDAVYQSLLKKEKERLETELTLKLESAHSEKLNKKEQELIKQEAEFSAYKKQV
                     ASEKENEVLKIKQTLMAEFNEKLNEKEKELTKEKTDFANYKTQVEEKHALALQVKELE
                     LNEQIIQKQIEIDNLKREQSLASVKVIGENLEKWCAEEYQKYAIQGFQNCTWEKDNDV
                     IKWAGEASGTKADFIFKVFNNEKKEKLMTTVVMDMKSEALVSKTKNKNSDHYKKLDLD
                     RQKKKGDYALLVSELELSSDNDSPIKKVQEYNNMYVVRPQYMMLFLSIITTISEKNKE
                     LHEEIAALKLKGEDTTKIFEAFDSFKNETLKIAIDILDKHLSDIETKTRTIQKSTESI
                     LKSTETITKIIKDNIIYRLENFNIQNILK"
     gene            complement(2579..3289)
                     /locus_tag="PLQ22_02500"
     CDS             complement(2579..3289)
                     /locus_tag="PLQ22_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOH58863.1"
                     /translation="MNVKFGLVSYKFINNDIEFNVNQIQKAIKKVSSVDMICFGEAFL
                     QGFDAFNWEYSHDKDIAITQDSKTMKRLEEISFNNKVDLGIGYLEKDEDKLYCSYAII
                     IDGKLKYNYRRISKGWKDFRKTDYHYQEGNETLKFNYKGLEIVVALCGDLWDYPDKFK
                     AKDLLIWPIYANFTLKQWTEYEVEYAEQAYKVASKTLMINSLSCEPKSYGASFYFEKG
                     KVVKKLEYNQEGILVINI"
     gene            complement(3304..3636)
                     /locus_tag="PLQ22_02505"
     CDS             complement(3304..3636)
                     /locus_tag="PLQ22_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOH58864.1"
                     /translation="MDNKKDNRYYLEKILIDLKFIIDHTKDKTLEEIASNEILIDSIM
                     FRIIQVAESNNYLTDDFKEQHKEVPWTAIRGMRNKIVHDYSIINMAIVYDTVKNGIPE
                     MYAILSKI"
     gene            complement(3629..4099)
                     /locus_tag="PLQ22_02510"
     CDS             complement(3629..4099)
                     /locus_tag="PLQ22_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH58865.1"
                     /translation="MKLKKLRQEKGLTQEQVAQLLGVTRRTYLKYENDQSSLSEIKYK
                     FIYQTLEQYGVIDEERGILTIEQIKNACFDIFKDYDVEYCYLFGSYAKGNANPKSDID
                     LLISVNASGLQFFEIVERLREKLKKKIDLLDAKELEKNILLVEEILKDGVKIYG"
     gene            complement(4259..5455)
                     /locus_tag="PLQ22_02515"
     CDS             complement(4259..5455)
                     /locus_tag="PLQ22_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH58866.1"
                     /translation="MNTKKMTDDIIEETDKIIKKFGTRMAGTKASLDTADYLYEDAKG
                     YSDSTHVDNFYIHKGAFLGWINILVLGYIVGFVFFIFKLEIVTISIAVASIVILYLQF
                     IRYLPIIDFLFPRKKARNVYGVIEPKGEVKRQIIISGHHDSAPVFNFLVYQPKLYNLR
                     VTGSIVFVILGLVLSIVNLFINNDILYYASIGVIGLGVLLVSQMWFFRGRKATPGAGD
                     NLASTQTAFQMGKYYYSLKGTDKELKHTRIIFASFDAEEEGLRGARAFVKKNKDKLLD
                     VKTYVLNIECLYDENALFFLTSDINDSVKLSDDLASKLVDIAAKNNVKTHKQPIAVLT
                     GGTDAGEFGKIGVEATTIMGMPWSNDNRSMAYHTPNDTIDKVSKTAVEHTINIFHNFI
                     VSKDQE"
     gene            6011..>6359
                     /locus_tag="PLQ22_02520"
     CDS             6011..>6359
                     /locus_tag="PLQ22_02520"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HOH58867.1"
                     /translation="MHGYYKRSIKIPLGKIALSILRVKCKSCGKTHAILHPSIIPYSQ
                     IPLCDTIEIIDAYENNKSFTNILNNNPDITESDVRYTIKRYLKCWKDRILSISTIPIL
                     DFILLADFQKKVSD"
BASE COUNT         2069 a         1101 c          648 g         2541 t
ORIGIN      
        1 taatcaaaaa cataagcacc atatttatct tccggcatct ctttgataaa agtttggtaa
       61 acttcatcaa tttcattttt tatcccaaaa gaagttaatt gctcttgtct tttaactaaa
      121 ttttctaata aagcaaaaat atttatatgg ccatctttat aggcatattg caaagcatct
      181 tgtttttctc tcatagacat cgtttccatg aaagaagcta tatttattcc agtttcgtca
      241 attgtaattg gattacctgt aagcatatca ttttgcatat catcaacata attagttact
      301 ccttgcgata atgaaagtac cgatgcaatt ccaataattc caatagatcc agcaatacac
      361 actaaaacag ttcttcttaa ttttgaaatt aggtttcttg aagaaagttt aatcgctgac
      421 aaaattgata acttcgattt tttatcatat ttaaacttct cttaaattat taattctgct
      481 ggttcgtgcg gattactatc acttattact aatccatctt ctaactttat aattcttgta
      541 gagtactcat aagcaatctt agaattatga gtaaccataa ttactaatct ctctttagaa
      601 atttctttca tcaattccat aatctgttta gatgtttctg tgtctaaggc tcctgtaggt
      661 tcatcagcta ataaaatctc aggattatta atcaaagctc tagctattga tactctttgt
      721 tgctgacctc ctgataattg attaggtctt ttattgtatt ctccttgtag accaacttta
      781 tctaaaactt caattgcttt tttccttctt gtttccttat caacacctgc aatattaagg
      841 gatagctcca cattttctaa aacagtttga tgaggaatca aattataact ttggaaaaca
      901 aagccaatcc tatgattacg gtaagtatca aaatcacgat ctgtaaattt atctgtcggt
      961 ataccatcaa tatacaagtt cccacttgtg tagtgatcta accctccaat tatatttaat
     1021 aaagtagtct ttccacaccc tgaaggtcct aaaattgaaa caaattcagc ttttctaaaa
     1081 gataaattaa tacctcttaa agcatgaact tttccccaac taactttata atcttttgta
     1141 atgttctcta atctcaacat acaatcacct tttcctctta ataatacata tgtttattat
     1201 atcataatct atagtctttt tctattcaaa tactgaaaat ttgaaaatta aaaatccggc
     1261 taataaaaat agccggattg tttactttca aaaaagttat tttaaaatat tttgaatatt
     1321 aaagttttct aatctataaa taatattatc tttaataatt tttgttattg tttctgtact
     1381 ctttaatatt gactctgtac tcttttgaat agttcttgtc tttgtttcaa tatcactaag
     1441 atgtttgtct aaaatatcaa tagcaatttt caatgtttca tttttaaaag aatcaaaagc
     1501 ttcaaatatt ttagtagtat cttctccttt taattttaat gcggcaattt cttcatgcag
     1561 ttctttattc ttttcagaaa ttgttgtaat gatacttaag aataacatca tatattgtgg
     1621 tctaactaca tacatattgt tatattcttg aacttttttg attggtgaat cattatctga
     1681 acttaactct aattcactta caagcaatgc ataatcacct tttttctttt ggcgatctaa
     1741 atcaagcttt ttatagtgat ctgagttttt gtttttagtt ttactcacta aagcttcact
     1801 cttcatatcc ataactactg ttgtcattaa cttttctttt ttctcgttat tgaatacttt
     1861 aaaaatgaag tctgctttag ttcctgatgc ctcaccagcc catttaatta catcattatc
     1921 tttttcccaa gtgcaatttt gaaaaccttg gatagcatat ttttgatatt cttcagcaca
     1981 ccatttttct aaattctcgc caatcacttt tacgctagcc aaagattgtt ctctttttaa
     2041 attatcaatt tcaatttgtt tttggataat ttgttcgttt agttctaact cttttacttg
     2101 taatgctagt gcatgttttt cttcaacttg agttttatag tttgcaaagt ctgtcttttc
     2161 tttagttaat tctttttctt tttcgttcaa tttttcatta aattcagcca tcaatgtttg
     2221 cttaatctta agtacctcat tttctttttc acttgctact tgttttttat aagcactaaa
     2281 ttctgcttct tgtttaatta actcttgttc ttttttgttt aacttctcgc tatgagcaga
     2341 ttctaacttc aatgttaatt cagtttcaag acgttctttt tcttttttaa gtaaactttg
     2401 gtaaacagca tctttcccct ctttaatttc cttttcaata attcctaaat ctaattgaat
     2461 ttgatctaaa ttttttaaat caattacatc acctttagaa caatcttcta acaattctaa
     2521 ttcgtgactt gataaaattt taactttctt aattttactc atattttctc ctttctgttt
     2581 aaatatttat aactaatatt ccttcttggt tatattctag ttttttaact actttccctt
     2641 tttcaaaata aaatgaagca ccataagatt ttggctcgca acttaatgaa ttaatcatta
     2701 aagttttact tgctacttta tatgcttgtt ctgcatattc tacttcgtat tctgtccatt
     2761 gcttcaaagt aaagttcgca tagattggcc aaattagtaa atccttagct ttaaacttat
     2821 caggataatc ccataaatct ccacataaag ctacaactat ttctaatcct ttataattga
     2881 attttaatgt ttcatttcct tcttggtaat gataatctgt ttttctaaaa tctttccagc
     2941 cttttgatat tctacgataa ttatatttta acttaccgtc aataataatt gcatatgagc
     3001 aataaagttt atcttcatct ttttctaaat aacctatgcc taaatctact ttattattaa
     3061 atgatatctc ttctaatctt ttcatagttt tagagtcttg tgtaattgca atatctttat
     3121 catgactata ttcccaatta aaagcatcaa aaccttgcaa gaatgcttca ccaaaacata
     3181 tcatatctac actactaact tttttaattg ccttttgaat ttgattaaca ttaaattcaa
     3241 tatcattatt tataaattta tatgaaacta atccaaattt cacattcata atttttcctc
     3301 aaattaaatc ttgcttaaaa tagcatacat ttctggtata ccatttttga ctgtatcata
     3361 cacaatcgcc atattgataa tactatagtc atgaacgatt ttatttctca tacctcgtat
     3421 tgccgtccaa ggaacttctt tatgttgttc tttgaagtca tctgttaaat aattattgct
     3481 ttctgctact tgaattatac gaaacataat tgaatcgatt aaaatctcat ttgaagctat
     3541 ttcttctagt gttttatctt tagtatggtc aataataaat ttaagatcta taagaatttt
     3601 ctctagatag tatctattgt cttttttatt atccataaat ctttactcca tctttcaata
     3661 tttcctctac taatagtata tttttttcta attccttagc atctaataaa tctattttct
     3721 tttttagttt ttcgcgcaat ctttctacaa tctcaaaaaa ctgtaatcca ctagcattta
     3781 cagaaatcaa taaatctata tcacttttag gattagcgtt gcctttagca tatgaaccaa
     3841 ataaataaca gtattcaaca tcataatctt taaaaatatc aaaacaagca ttttttattt
     3901 gctcaatggt tagtatccct cgttcttcat ctatcacacc atactgttct aatgtttgat
     3961 aaataaactt atattttatt tcagataagc ttgattgatc attttcatac tttaaatatg
     4021 ttctacgcgt tacccctagt aactgtgcaa cttgttcttg agtaaggccc ttttcttgtc
     4081 ttaatttctt taatttcatt tgtatcacct caattatatt atatcacctt tcacatataa
     4141 gtcaatgata atgtgaactt ttttcacata aatttttctt ttaatgtgaa ttattttcac
     4201 ttcatttaaa tgaaaagcga aacaaaaaca cctatatttt ataggtgttc atttatgact
     4261 attcttgatc ttttgaaaca atgaaattat gaaagatatt aatagtatgt tctactgctg
     4321 tttttgatac tttatctatt gtatcgttag gtgtatgata tgccatacta cgattatcat
     4381 tactccaagg catacccata atcgttgttg cttcaactcc aatttttcca aactcaccag
     4441 catcagttcc accagttaaa acagctattg gttgtttatg tgtcttaaca ttgtttttgg
     4501 ctgcaatatc aactagttta gatgctaaat catcacttaa cttaacgcta tcattaatat
     4561 cacttgttaa gaaaaataat gcattttcat catacaaaca ttcaatattt aaaacatagg
     4621 tcttaacatc taacagttta tctttatttt tctttacaaa agctcttgct ccccttaatc
     4681 cttcttcttc cgcatcaaat gaagcaaaaa taattcttgt atgttttaat tctttatctg
     4741 ttccttttaa actataataa tatttaccca tttgaaaagc agtttgggtt gaagctaagt
     4801 tatctcctgc accaggagtt gcttttctgc ctctaaaaaa ccacatttgc gatactagta
     4861 aaacacctaa gccaataact ccaattgaag cataatataa aatatcatta tttataaaca
     4921 agtttacaat cgataatact aaacctaaaa taacaaaaac aattgatcca gtaactctta
     4981 aattataaag ctttggttga taaactaaaa aattaaacac tggtgcacta tcatggtgtc
     5041 cactaataat aatttgcctt ttaacttcac ctttcggctc aattacccca taaacatttc
     5101 ttgctttttt ccttgggaac agaaaatcaa taataggcaa atacctaata aactgcaaat
     5161 aaagaattac tatactagca acagctatag aaatcgttac tatttctaat ttaaaaatga
     5221 agaaaacaaa cccaacaata tagcctaata ctaaaatatt aatccatcca agaaaagctc
     5281 ctttatgaat ataaaaatta tctacatgag tactatcact atatccttta gcatcttcat
     5341 acaaataatc tgctgtgtca agtgatgctt tagttccagc cattcttgtt ccaaatttct
     5401 taattatctt atcggtttct tcaataatat cgtctgtcat tttttttgta ttcattttat
     5461 aatcccccat taaatatctt tcttttttaa aaaacttcaa aatcttttct aatagttatt
     5521 atataacact tatcttatat tcaccttaac atctccaaat attctaaatc tattgataag
     5581 ttataatatc acttaatcat tcaaatgtca attacgcaac gtaaaactaa tatatacaaa
     5641 aagtccaatt taaaattgga ctttattttt aatctggctt tgattaccta tagatagtaa
     5701 cattatatga agctaattca ttaataaata atcaaattaa attcagtaaa agccatcatt
     5761 ttatgactta attctagtca aattatttga tggctctttt aatattttcc aacatttggt
     5821 ataataaaat aaaaaaatga agaattcaaa tctccacaga tcattcttca tttaccacaa
     5881 aggcaatatt atgttatcac aagttacagt taaaatcaag tacctaactc agaaaaatta
     5941 taataagtat ctatatttac taaacttttc gacttgtaaa tgttcatgtg gagctacagg
     6001 tcgttttgtt atgcatggat attataaaag gtcaattaaa ataccacttg gtaaaatagc
     6061 actatctatt ctccgtgtta agtgtaaatc atgcgggaaa acacatgcca tattacatcc
     6121 tagtattatt ccatattctc aaataccatt gtgtgatact atagaaataa ttgatgcata
     6181 cgaaaataat aaatcattca ctaatatatt aaataataat cccgatatta cagaaagtga
     6241 tgtgagatat accattaaaa gatatttgaa gtgttggaaa gatagaatct tatcgatttc
     6301 aacaattccg atattagatt ttatattatt ggctgacttt caaaaaaaag tatctgatt
//
LOCUS       DAOSGS010000040         6303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_10878_length_6303_cov_5.326985, whole genome shotgun sequence.
ACCESSION   DAOSGS010000040 DAOSGS010000000
VERSION     DAOSGS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10878_length_6303_cov_5.326985"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..931)
                     /locus_tag="PLQ22_02525"
     CDS             complement(110..931)
                     /locus_tag="PLQ22_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH58868.1"
                     /translation="MNKEISKIIQEFKKDKEISAFIKKHKLSDEDILNNFGTFFDIYN
                     HHQLLKENMQKYIEEYGNNTVELDYTDGKISLVSAPLQGEGTGLLEFMYYDFEPETGE
                     LYKNDARMMVLTKVAQFIQNYQEKKYTKGLYIYGSYGVGKTYIAMHLAQIISKTEKVL
                     FVFFPDLVRNLKTSFQESNTEQLILKIKQAPVLVLDDFGVSSLTPFVRDDALVAILQY
                     RMTMNLPVIFTSNSSLSEIRKELVASADIAGNRLYTRIKEMVDEVKLDDKNYRLR"
     gene            complement(937..2244)
                     /locus_tag="PLQ22_02530"
     CDS             complement(937..2244)
                     /locus_tag="PLQ22_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH58869.1"
                     /translation="MKHTNFRVYKNFELSGEAGFCLTKLYLPLIGIDSFALYNLLMTY
                     DNKETYDIRTVLDSLNFNNDHFFQEALNKLNALSLVETYFDEEKGYYLYLKQPLAVES
                     FLSNRLLVKFLTEKIGPVEVENITKNLNYKIRGYTDITKTFDEVYVKDFKEEVDPFAK
                     LFFNKIKDNINIKGEKFDYLLFKMEFDENIIPSAVFDDPELKNKIINVAYTYDLNVQE
                     MKQVIEDVVLQNNDLPLSKIGESAEKIYTLKAQSQNKKVQSRFVTKEPDAFVSSKQDD
                     TLFELVNILENNSPKNILESLSGITASKTELRTLERLTSELGLARGVVNVLLYYVLNE
                     KEGTMPSYNYFEKVANEWARKGVKDTKSALLHTKQLKEQPKGGAVKYTNKKTGVRPDW
                     MDQYEEETEAKDKEKQKQEQEYFDNLSEEEKAKLEEKIKKVLG"
     gene            complement(2254..2616)
                     /locus_tag="PLQ22_02535"
     CDS             complement(2254..2616)
                     /locus_tag="PLQ22_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58870.1"
                     /translation="MGFKEFFSNNFETKENHHLQSLRTRYYRNSLADVRKAVYDLMKE
                     ENGKLISDNKQFNEILFETNKYSLIVILVETKINEIAIDFAITTKFIFPRGRGQKIIE
                     RLYQVLDKKLNYKGVSLY"
     gene            complement(2618..3187)
                     /gene="coaE"
                     /locus_tag="PLQ22_02540"
     CDS             complement(2618..3187)
                     /gene="coaE"
                     /locus_tag="PLQ22_02540"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOH58871.1"
                     /translation="MVIGITGSIASGKTKVANYLKSLGYKVLSADEENSKLLEDPHVI
                     LEIKEKVSSDVVVSGKVDKKLLAKLVFNNKEALKKLTDITHPLIYLSLKMQIQKNQLT
                     FIEVPLLVETNFTELVDKIMVIDIQEKKQIKRLMKRNQMGEEEAKQLISLQMPSSEKA
                     KYGDYVIDNSRCFCNTKRQINKILKEIGG"
     gene            complement(3190..>3803)
                     /gene="mutM"
                     /locus_tag="PLQ22_02545"
     CDS             complement(3190..>3803)
                     /gene="mutM"
                     /locus_tag="PLQ22_02545"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOH58872.1"
                     /translation="ISHLRMEGKYFLRGNEEKEKHEHIIFYLKSGKTLRYHDVRKFGT
                     MHLYLDLSYEELLKKEPLNKLGLEPFSNSLNTEYLKDKLKTKSQAIKQLLLDQSIITG
                     IGNIYADEILFMAKINPKKAGKLLNNDELEKIIKYTKVVLEKAIQLGGTTIRSYSSDG
                     ISGLFQNELLVHTKEKCPVCGKDITKIKIGGRSTYYCEYCQKE"
     assembly_gap    3804..3903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3904..4376)
                     /locus_tag="PLQ22_02550"
     CDS             complement(<3904..4376)
                     /locus_tag="PLQ22_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH58873.1"
                     /translation="MKEIKIRRSVRSFEDKLLTDKELEKLVRSGMQAPSARNQQPLYY
                     IAINKKEVLKEISEKVPGYKMVGEAGGLIIIFVRNEEHPAPKQTEQDASAATQNILLE
                     ATHLGIGSCWLSTFHNEAKQEAIANYFKLPEKHLPAVGIALGYPKDKDALKFIDR"
     gene            complement(4388..>6303)
                     /gene="polA"
                     /locus_tag="PLQ22_02555"
     CDS             complement(4388..>6303)
                     /gene="polA"
                     /locus_tag="PLQ22_02555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOH58874.1"
                     /translation="EEAYKTKYLATLVKDAYIPYKIEDLNYQNYEHPKLYRFYKEMEF
                     NSLMKQSDQGELEENPNLEIIEDLNYDFSKLDKEITLSLETYYENYYNNQILGLAIKD
                     KKHHLFVSKDVLLQNKSLHNILGNEETKINLFDYKKTYVLLKKRGINLKNVTFDLLLA
                     AYLINPSYSNDDIQVVAQNFLETNIPHYDTIYKENKKYHIPSLDIYSQYAAAKNDLIN
                     KLKKQVLAEIKENEIEDLFKIEIELSKVLGDMEICGLLIDPLKLKEIGEEFEVEASKY
                     QDEVFMYAGEKFNLNSYKVLGEILFEKLKLPVYKKTKTGYSTNSEVLEKLAYRYPIAK
                     AILEYRSYNKLITTYVNGLLEVKDENNYLHPVYKQALTTTGRLSSIEPNIQNIPIRTE
                     TGQTIRKVFVSRFKDGLILSADYSQIELRVLASLSEDPKMIEFYNQGLDIHRATAASI
                     YGIDYNMINSSQRRDAKTINFGIIYGMSAWGLSEALNINPKDASDYIERYFEIFKGAK
                     EFLDNQVANAREKGYTKTLLNRRRYIDNINSPNINLRNFGERTAMNAPIQGTAADIIK
                     LAMNKVKAKMVEAKLESLLIAQVHDELVFDVKPNELEVLKDIVKTEMENVIKLNVPLV
                     IEINYGKNWYETK"
BASE COUNT         1946 a         1021 c          691 g         2545 t
ORIGIN      
        1 tcaccaacca aatactctct gactttacta gtattaataa tcgtgtccac ttctttgttt
       61 attaagatta aattaattat atcacatttt ttaaagaaaa ccaataaaat tatctcaaac
      121 gataattttt atcgtctaat tttacttcat caaccatttc tttgattcta gtatacagtc
      181 tattacctgc gatatcagcg ctagcaacta actcttttct aatctctgat aaagacgagt
      241 ttgaagtgaa gataactggt aagttcattg tcatacgata ttgtaatatt gcgactaaag
      301 catcatccct tacaaaagga gttaatgaag acactccaaa atcatcaagc actaaaacgg
      361 gtgcttgttt tatttttaga attagttgtt cagtattgct ttcttgaaaa gaagttttca
      421 aattacgaac taaatcagga aagaaaacaa acaatacttt ttctgtttta gaaatgattt
      481 gggctaaatg catagcaata taagttttac caacaccata agaaccataa atatataaac
      541 ctttagtata tttcttctct tgataatttt gaataaattg tgctactttt gtaagcacca
      601 tcattctcgc atcgtttttg tatagttctc cagtttccgg ttcaaaatca taatacataa
      661 attctaataa gccagtacct tctccttgaa gtggagcaga tactaaacta attttaccat
      721 cagtatagtc aagctctact gtattatttc catactcttc aatatacttt tgcatatttt
      781 cttttaataa ttggtgatga ttgtagatat caaaaaacgt tccaaagtta tttaagatat
      841 cttcatcaga aagtttatgc ttttttataa aagcactaat ttctttatct ttcttaaatt
      901 cttgaatgat tttactaatt tctttattca tttacatcac cctaatactt ttttaatttt
      961 ttcttctaat ttagcctttt cttcttcgct taagttatca aaatattctt gttcttgttt
     1021 ttgtttttct ttatcttttg cttctgtttc ttcttcgtat tgatccatcc aatcaggtct
     1081 tactcctgtt tttttattag tatattttac agctcctcct tttggttgtt cttttaattg
     1141 ctttgtatga agtaaagcac tttttgtatc tttaactcct ttacgagccc actcattagc
     1201 tactttctca aagtaattat atgaaggcat tgttccttct ttttcattta aaacatagta
     1261 aagcaaaaca tttacaactc ctctagctaa acctaactct gaagttaatc tttctaaagt
     1321 tctcagttca gttttagatg cagtaattcc acttaaactt tctaaaatat tttttgggga
     1381 attgttttct aaaatattaa ctagttcaaa taaggtgtca tcttgtttac tagaaacaaa
     1441 agcatctggt tctttagtta caaaccttga ttgaactttt ttgttttgac tttgagcttt
     1501 taaagtataa atcttttctg cgctttcacc aatctttgat aatggtaagt cattattttg
     1561 aagaactaca tcttcaataa cttgtttcat ttcctgaaca tttaaatcat aagtataagc
     1621 tacattaata attttattct ttaactcagg atcatcgaaa acagcacttg gaatgatatt
     1681 ttcatcaaac tccatcttaa ataataaata atcaaacttt tcacctttaa tattgatatt
     1741 atctttaatt ttattaaaaa acaacttcgc aaaaggatca acttcttctt taaagtcctt
     1801 cacatatact tcatcaaaag tcttagtaat atctgtataa cctctaattt tataatttaa
     1861 gtttttagtt atattttcca cttcaactgg acctattttt tcagtcaaaa acttaactaa
     1921 aagtctattt gataaaaagg actcaacagc taatggttgc tttaaatata aataatagcc
     1981 tttttcttca tcaaaataag tttcaactaa acttaaggca tttaatttat ttaaagcttc
     2041 ttggaaaaag tggtcattat taaagttcaa actatcaaga actgttctta tatcataagt
     2101 ttctttattg tcataagtca ttagtaaatt gtataaagca aaactatcaa tcccaatcaa
     2161 aggtaggtat agtttagtta aacagaatcc agcttcccct gataattcaa aatttttata
     2221 aactctaaaa tttgtatgtt tcatatattg ctcctaatat agactaacac ctttatagtt
     2281 tagttttttg tctaaaactt ggtatagcct ttcaataatt ttttggcctc ttccacgagg
     2341 aaaaatgaat tttgtagtga tagcaaaatc tatagcaatt tcattaatct tcgtctctac
     2401 aagaattact attaatgaat atttatttgt ttcaaataaa atttcattaa attgcttatt
     2461 atctgaaatt aattttccat tttcttcttt catcaaatca taaactgctt ttcttacatc
     2521 agctaaacta ttacggtaat atcttgttct taatgattga agatgatggt tttcttttgt
     2581 ttcaaaattg ttactaaaaa attctttaaa acccatttta ccctcctatt tcttttaata
     2641 ttttatttat ttgtcttttg gtattacaaa agcaacggct attatcaata acataatcac
     2701 catacttagc tttctcacta cttggcattt gcaagctaat taactgttta gcttcttctt
     2761 cacccatttg atttcttttc attaatcttt taatttgttt tttttcttga atatcgataa
     2821 ccattatttt atctacaagt tcagtaaagt tagtttctac taataaagga acttctataa
     2881 acgttagttg atttttttga atttgcattt ttaaactcaa ataaattaaa ggatgagtta
     2941 tatcagtaag tttttttaaa gcttctttat tattgaaaac aagttttgct agaagcttct
     3001 tatctacttt ccctgaaaca actacatcag aacttacctt ttcttttatt tctaaaatta
     3061 catgaggatc ttctagtaac ttactattct cttcatccgc tgataaaact ttatagccta
     3121 aactctttaa atagtttgct actttagttt tacctgaggc tattgagcct gtaattccga
     3181 taaccatctt tactccttct ggcaatattc acaataatat gtgcttctac cacctatttt
     3241 tatttttgta atatctttcc cacacacagg acatttttct tttgtatgta ctaaaagttc
     3301 gttttggaat aaaccactaa tcccatctga agaataagat ctaattgttg tcccacctaa
     3361 ttgaattgcc ttttctaaaa caacttttgt atatttaata attttctcaa gctcatcatt
     3421 atttaataat ttaccggcct ttttaggatt aatctttgcc ataaataaga tttcatcggc
     3481 gtaaatgttt cctattcctg taattatgct ttggtcaagt aataattgtt tgattgcttg
     3541 actttttgtt ttaagtttat cttttaaata ttcggtattt aaactattac taaaaggctc
     3601 taatcctaat ttatttaaag gttctttttt taataattct tcataactta aatcaaggta
     3661 taaatgcata gttccaaatt tacgtacatc atgatatctt aatgtctttc cgctctttaa
     3721 ataaaagata atatgttcat gtttttcttt ttcttcattt cctcttaaaa agtatttacc
     3781 ttccattcgc aaatgagaaa ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnntaacgat caataaactt taaagcatct ttatcttttg gataacctaa agcaatcccc
     3961 acagctggaa gatgtttttc tggtaattta aagtaattag caattgcttc ttgttttgct
     4021 tcattatgga aagtagatag ccagcatgaa cctattccta aatgagttgc ttcaagtaaa
     4081 atattttgtg ttgcagctga agcatcttgt tcagtttgtt ttggtgctgg atgctcttca
     4141 tttcttacaa agataatgat taatcctcca gcttcaccta ccatcttata accaggtact
     4201 ttttcagaaa tttcttttaa aacttctttt ttgtttatgg cgatgtagta tagaggttgt
     4261 tggtttcttg cactaggtgc ttgcattcct gatcttacta gtttttctag ttctttatca
     4321 gttaatagtt tatcttcaaa cgatctaaca cttcttctaa ttttaatttc tttcataatt
     4381 acctccccta ttttgtttca taccaatttt tcccatagtt aatctcaata actaatggta
     4441 catttaattt aattacattt tccatctcag tctttacaat atcttttaaa acttcaagtt
     4501 cattaggttt tacatcaaac actaattcat catgaacttg agcaattagt agtgattcta
     4561 acttagcttc aaccatttta gcttttactt tattcatcgc tagtttaata atatctgctg
     4621 ctgttccttg aattggtgca ttcatcgctg ttctttcccc aaagtttctt aagttgatat
     4681 ttggactatt gatgttatca atatatcttc tacgattaag taaagtctta gtataacctt
     4741 tttctctagc atttgctact tgattatcta aaaattcttt tgcaccttta aaaatctcaa
     4801 aataacgttc aatataatca ctagcatctt ttggattaat gtttaaagcc tctgataaac
     4861 cccaagcact catcccataa ataatcccaa agttaatcgt cttggcatct cgtctttgac
     4921 tagaattaat catattataa tcaatcccat aaatactagc agctgtagct ctatgaatat
     4981 ctaaaccttg attataaaac tcaatcattt ttggatcttc agataaagat gctagtactc
     5041 ttagttcaat ttgcgagtag tcagcgctta aaattaaacc atctttaaag cgtgaaacaa
     5101 aaacttttct aatagtttga cctgtttctg ttcttatcgg aatattttga atattaggtt
     5161 caattgaaga taatctacct gtagttgtta gggcttgttt ataaacagga tgtaaataat
     5221 tgttttcatc ttttacttct aataagccat ttacataagt agtgattaat ttattataag
     5281 atcgatattc taagatagct tttgctattg gatatctata agctagcttt tctaaaactt
     5341 ctgagtttgt agaatagccg gttttagttt tcttataaac aggtaacttt aatttttcaa
     5401 ataaaatctc acctaaaact ttataagaat ttaagttaaa cttttcacca gcatacataa
     5461 atacctcatc ttgatattta ctagcttcaa cttcaaactc ttcaccaatc tcttttaatt
     5521 ttaatgggtc aattaacaaa ccacatattt ccatatcccc tagaacttta gataattcaa
     5581 tctcaatttt aaataaatct tcaatttcat tttctttgat ttcagctaaa acttgtttct
     5641 ttagtttatt aattaaatca tttttagctg ctgcatattg agaatagata tctaaacttg
     5701 gaatatggta ctttttattt tctttatata ttgtatcata atgaggaata tttgtttcaa
     5761 gaaagttttg cgctacaact tggatgtcat cattactata actagggtta attaagtaag
     5821 cagctagtaa taaatcaaat gtaacatttt ttaaattgat tcctcttttt ttaagtaaaa
     5881 cataagtctt cttgtaatca aataagttaa tctttgtttc ttcattacct aatatattat
     5941 gtaatgactt gttttgtaat aaaacatctt ttgatacaaa aagatgatgt tttttatctt
     6001 taattgcaag tcctaaaatt tgattattat aatagttttc ataatatgtt tctaaagaca
     6061 gagtgatttc tttgtctaac ttactaaaat cataatttaa atcttcaata atttctaaat
     6121 taggattttc ttctaactca ccttgatcac tttgtttcat taaactatta aattccattt
     6181 ctttatagaa acggtatagt ttaggatgtt catagttttg ataatttaaa tcttcaattt
     6241 tataaggaat ataagcatct tttactaatg tagcaagata tttagttttg taagcttctt
     6301 ctt
//
LOCUS       DAOSGS010000041         6279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_10940_length_6279_cov_4.039685, whole genome shotgun sequence.
ACCESSION   DAOSGS010000041 DAOSGS010000000
VERSION     DAOSGS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10940_length_6279_cov_4.039685"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1301
                     /gene="gap"
                     /locus_tag="PLQ22_02560"
     CDS             288..1301
                     /gene="gap"
                     /locus_tag="PLQ22_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH58875.1"
                     /translation="MAVKVAINGFGRIGRLAFRLMYGDPDFKIVAVNDLTDAEQLAYL
                     LKYDSTQGRYKADKIKHEGDEIIIGKDRIKVFAKMDPAELPWKELDVDVVMECTGVFT
                     KEEGAMKHINAGAKKVIISAPGKGPNVKTIVYNVNHDILDGSEKVISAASCTTNCLAP
                     MAKILEDKFGIVKGFMTTIHAYTGDQVTLDVAHKKGIASRRGRAAALNIVPTTTGAAS
                     AVGKVIPALLGKLDGIAMRVPTGTGSAVDLVVELKKKVTVEAVNKAMKKAADESFGYT
                     EDPIVSSDIIGDHHGGVLDAASTCLLEVDGKQLVKLIAWYDNEMGYTAQMVRLAKHIA
                     HQK"
     gene            1374..2711
                     /gene="rlmD"
                     /locus_tag="PLQ22_02565"
     CDS             1374..2711
                     /gene="rlmD"
                     /locus_tag="PLQ22_02565"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOH58876.1"
                     /translation="MKVNEVLKLKATDLNHNGYGVARHNNLVVFVPYLLKNEEANVKI
                     ISVKKNYSFGVIDKITKPSKDRVEPVCKHYYECGGCSLLHLNYEAQLKHKLDSAQRTL
                     KKLGNIDLEIKDIYGKDQYHYRNKVQMFYSQDKQIYSGFYQEQTHDVFKLEECYLQTK
                     TASKISVTLDKILNKYQVPIYNEKTKAGLLRAILIRNNQKGEYVVVLISKEDKINNLD
                     RIVKELVKEHPEIIQVHININSKSTNVILGSKTKELYFKKKFIEEIFDLKFELNYNSF
                     FQINYDVMLMLYQEIVDKVKNLKIKKAIDAYCGVGTISLLLSKYVSEVIGIEVVFEAI
                     KNADNNKKINNIKNAKFILGKTEEKLLSINNIDLLVVDPPRKGLEDSLIDTIKQKEIK
                     HIIYVSCNEATLARDLGLLKDEYNIKEIKAFDMFPNTNSLECLAYLERKPKAK"
     gene            2797..4038
                     /locus_tag="PLQ22_02570"
     CDS             2797..4038
                     /locus_tag="PLQ22_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH58877.1"
                     /translation="MKVLKGAALFGQSGGPTSVINSSAAGVFLEALNQENITHVYGAE
                     YGIKGILDDRLFDISKEDRDELELLKNTPSSALGTVRYKLADYKKDDTDYKRLLEIFK
                     KYNIRYFFYNGGNDSMDTCNKISKYLQKEGYPCRVMGVPKTIDNDLEGTDHCPGYGSA
                     AKYVATTMMELYLDTHVYNTPLITVVEIMGRNAGWLTAAAELATYKGYGPDLMYLPET
                     PFDLTEFTKEVKEVAKRKEGKVLIAVSEGIRDKDGKYISEYASSVSRDSFGHAQLGGL
                     AAFLANHLNAHTNIKVRDIEFSLMQRCAAHIASETDIWEAFMAGKKAVEYACEGHTDK
                     MITFKRNEGKEYSISYEMLDLEHIANAEKKVPLEWIKKDHTGVTKEFIEYALPLIQGD
                     PKLKKEDGLPRFAKLEKKLVK"
     gene            4120..5658
                     /gene="cls"
                     /locus_tag="PLQ22_02575"
     CDS             4120..5658
                     /gene="cls"
                     /locus_tag="PLQ22_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOH58878.1"
                     /translation="MKTIKKLLRTFLNKWVLFAFIIFLEIIALVLVFIYAGLSAGTTV
                     IITSIIHLVLVAISIVSLVSRKEYHSRYIIAWLVLVAVIPVAGSLFYLFTANTLSHKA
                     RQRKYKINSDKIKKVTDEIAEEYDVDPYFNSIQNLVRSRSHLPAWDKTDTKIYNRGED
                     MYEDIKEALLSAKEYIFLEFFIVRKGKMLTEILEIIQAKLKEGVKVYFVYDNFGGSRD
                     LPFRFKQKIRKMGVKTKTFNRIFPTLNLSFNYRDHRKIIVVDGKIAFTGGINIGDEYI
                     NREIRFGYWKDGGIRLKGKGVANFVLIFLNVWESLTGEHLDSKKFIKNEEVSDSEGYV
                     ISFQDGPLNYNHIIHEVYLTMINTAEKYIYISTPYLILDDEILLALKIAAARGIDVRI
                     ITPKMPDKKFVFMVTKENYKPLIEAGVRIYEYTPGFIHTKAVVSDDDIGTIGTSNFDF
                     RSFYLHYEDISLIYKTKSVKDLRNDFIETFAISEKIEAKDIKRNNIFKRALLVVLKIL
                     SPLL"
     gene            5670..>6279
                     /locus_tag="PLQ22_02580"
     CDS             5670..>6279
                     /locus_tag="PLQ22_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58879.1"
                     /translation="MKVRKILIGLFSLIFVFFGTIIINARPDAVPEDVTVSSGAYYFV
                     EETRQTNHLPYGITHKSDWAYSHEPLADGSVPTIDSYLSQQVNMLEIPKDANVSIVGW
                     ANTVDNRWTLTTVKRLAEQFERKYPEYKVIGAINGDGFDIRSQGAFPRQPQDITAGLG
                     NFFKSHLGDANGVLGFRRNGTTDTLVHIPNTAERVLTLEVIDE"
BASE COUNT         2379 a          720 c         1146 g         2034 t
ORIGIN      
        1 atagtttata tgatgcgatt ttatcggtaa aacctttaaa ggtgattgaa gttcatttaa
       61 ctgatcctaa aacaagagaa gattttagaa gaaaaagtgt tattgaagaa gtttgtttta
      121 aaacaatatc tggtttagga tttgaatctt ataaattagc aattgatgag tttttgtaaa
      181 acgtattaaa aacaaatata aagtgttttc actattgaaa atgattgaaa atatgatata
      241 atattatgta aagaaaaaat aaccatataa ggaggtattt gaaaaatatg gctgtaaaag
      301 ttgcaattaa tggatttgga agaattggaa gacttgcatt tagattaatg tatggtgatc
      361 cagacttcaa aattgtagct gttaatgact taacagatgc agaacaatta gcatacttat
      421 taaagtatga ctcaacacaa ggacgttaca aggcggataa aattaagcat gaaggtgatg
      481 aaatcatcat tggtaaagac agaattaaag tttttgcaaa gatggatcct gctgaattac
      541 cttggaaaga attagatgtt gacgtagtaa tggaatgtac tggtgtattt actaaagaag
      601 aaggtgcaat gaaacacatc aatgctggag ctaaaaaagt tatcatttct gctccaggaa
      661 aaggaccaaa tgtaaaaact atcgtttaca atgtaaacca tgatatttta gatggttctg
      721 aaaaagttat ttcagcagca tcttgtacta caaactgctt agcaccaatg gctaaaatcc
      781 tagaagataa atttggtatc gttaaaggat ttatgactac tattcatgct tacacaggtg
      841 atcaagttac tttagacgtt gctcacaaaa aaggtattgc tagcagaaga ggtagagcag
      901 ctgctcttaa catcgttcca acaactacag gtgctgcatc tgcagtaggt aaagttatcc
      961 ctgcattact tggaaaatta gatggtatcg caatgagagt tcctacagga actggttcag
     1021 ctgttgactt agttgtagaa ctaaagaaaa aagttacagt tgaagcagta aataaagcaa
     1081 tgaaaaaagc tgcagatgaa tcttttggtt acactgaaga tccaattgta tctagtgata
     1141 ttattggcga tcatcatggt ggtgttttag atgctgcatc tacatgctta ttagaagttg
     1201 atggcaaaca attagttaag ctaattgctt ggtatgataa tgaaatgggc tatactgctc
     1261 aaatggttag attagctaaa catattgcgc atcaaaaata ataaacaata aatcaaggtc
     1321 cacttgaatt agtgggcctt tttttatata atatataagt aaaagaggtc tatatgaaag
     1381 taaacgaagt actaaaatta aaggcaacag acttaaatca taatggttat ggtgttgcaa
     1441 ggcacaataa cttagttgtt tttgttcctt atttattaaa aaatgaagaa gctaatgtaa
     1501 agataattag tgtgaaaaag aattatagtt ttggggttat agataaaatt actaaacctt
     1561 ctaaagatag agtagaacca gtttgtaagc actactatga gtgtggggga tgttctttat
     1621 tgcatttaaa ttatgaagca caactaaaac ataaacttga ttcagcccaa agaacactta
     1681 aaaaactagg taatattgac ttggaaataa aagatattta tggtaaagat caatatcact
     1741 atcgcaataa agttcaaatg ttttatagtc aagataaaca aatctattca ggcttttatc
     1801 aagagcaaac tcatgatgtt tttaagttag aagaatgtta tctccaaact aaaactgcaa
     1861 gtaagattag tgttacttta gataaaattt taaataaata tcaagttcct atatataatg
     1921 agaaaactaa agctggatta ctaagagcaa tcttaattcg taacaatcaa aaaggtgaat
     1981 atgtagttgt tttaatttct aaagaagata aaataaacaa tttagataga attgtgaagg
     2041 agcttgtgaa agagcatcca gagattatcc aagttcatat aaatattaat agtaaatcaa
     2101 ctaatgttat tttaggatca aaaactaaag aattatattt taagaagaag tttattgaag
     2161 aaatatttga tttaaaattt gaactaaatt ataattcatt tttccaaatt aactatgatg
     2221 taatgttaat gctatatcaa gaaatagttg ataaagttaa aaatttgaaa atcaaaaaag
     2281 ctattgatgc ttactgtgga gttggaacaa taagtttatt gctttctaaa tatgtaagtg
     2341 aagttattgg aatagaagtt gtttttgaag caattaaaaa cgctgataac aataagaaaa
     2401 taaacaatat taaaaatgct aagtttattt taggtaaaac tgaagaaaaa ttactatcaa
     2461 taaataatat tgatttacta gtagttgacc cgccaagaaa aggcttggaa gattctttaa
     2521 ttgatacaat caaacaaaaa gaaattaagc atattattta tgtttcttgt aatgaagcaa
     2581 ctttagctag agatttagga ttattaaaag atgagtataa tataaaagaa atcaaagctt
     2641 ttgatatgtt tccaaataca aatagtttag aatgtttagc ttatttagaa agaaagccta
     2701 aggctaagta aaactataaa tattactaaa gagattgaaa attaaggcaa aatgtagtat
     2761 aatatataag atattattag aggaggaatt atataaatga aagttttaaa aggagcagct
     2821 ttatttggcc aatcaggtgg accaacatca gttattaact caagtgctgc cggagttttt
     2881 ttagaagcat tgaatcaaga aaacattact catgtttatg gagcagagta tggtattaag
     2941 ggtattttag atgatagact atttgatatt tccaaagaag atcgcgatga attagaacta
     3001 ttaaaaaata caccatcaag tgctttaggt acagtacgtt ataagttagc tgattataaa
     3061 aaggatgata cagattataa gagattatta gaaatcttta aaaagtataa tattcgttat
     3121 ttcttttata atggtggtaa tgattcaatg gatacttgca ataaaattag taaatatctt
     3181 caaaaggaag gatatccttg tagagttatg ggagtaccaa aaacaattga taatgactta
     3241 gaaggtactg atcattgccc tggatatggt agtgcggcta aatatgtagc aacaacaatg
     3301 atggaacttt atttagatac tcatgtttat aacacaccat tgattactgt tgtagagatt
     3361 atgggtagaa atgctggttg gttaactgct gcagctgaac ttgcaactta taaaggttat
     3421 ggaccagatt taatgtattt accagaaacg ccatttgatt taactgaatt tactaaagaa
     3481 gtaaaagaag ttgctaaaag aaaagaaggt aaagtgttaa ttgcagttag tgaaggtatt
     3541 agagataaag atggaaaata tatttctgaa tatgcatcaa gcgtaagtcg tgactcattt
     3601 ggtcatgctc aattaggtgg acttgcagct ttccttgcaa atcatttaaa cgctcataca
     3661 aatattaaag taagagatat tgagtttagt ctaatgcaaa gatgtgcagc tcacattgcc
     3721 agtgaaacag acatttggga agcttttatg gctggtaaga aagcagtaga atatgcttgt
     3781 gaaggccata cagataaaat gattactttt aaacgtaacg aaggaaaaga atatagtatt
     3841 tcttatgaaa tgttagattt ggagcatatt gccaacgcag aaaagaaagt tccacttgaa
     3901 tggattaaga aagatcatac tggagttaca aaagaattta ttgaatatgc tttaccttta
     3961 attcaaggtg atccaaaact taaaaaagaa gatggtctac cacgctttgc aaagttagaa
     4021 aaaaagctag ttaaataatt tgtaaaaagt agaatgcctt taaaaaataa aggcattttt
     4081 ttattgttta tgatataata tagatgtagg agaatttaga tgaagacaat aaaaaaactc
     4141 ttaagaacgt ttttaaataa atgggtttta tttgctttta tcattttttt agagataata
     4201 gcattagttt tggtttttat ctacgcggga ctaagtgctg gaacaaccgt aattattaca
     4261 tctattattc atttagtctt agttgcgatt tctattgttt cgttagtaag tagaaaagaa
     4321 tatcattctc gatacattat tgcttggctt gttttagtag cagttatacc tgttgcagga
     4381 tctttatttt atttattcac tgctaatact ttgtctcata aagctaggca aagaaaatac
     4441 aaaatcaatt cagataaaat taaaaaagta acagacgaaa tagctgaaga atatgatgtt
     4501 gatccttatt ttaatagtat tcaaaattta gttagatcaa gaagtcattt accagcttgg
     4561 gataaaactg atactaaaat ttataatagg ggcgaagata tgtatgaaga tattaaagaa
     4621 gccctactta gtgctaagga atacatcttt ttagaatttt ttattgttag aaaagggaag
     4681 atgctaacgg aaattttaga aattattcaa gctaaattaa aagaaggtgt caaagtttat
     4741 tttgtttatg ataactttgg tggctcaaga gacttaccat ttagatttaa acagaaaata
     4801 agaaaaatgg gggtcaaaac taaaacattt aatcgaattt tcccaacatt aaatctttca
     4861 tttaactatc gtgatcaccg aaagattatt gtagtggatg gtaaaatagc ctttaccgga
     4921 ggaattaata ttggtgatga atatatcaat agagaaatta gatttggtta ttggaaagat
     4981 ggaggaataa ggttaaaagg caagggagtt gcgaattttg ttttgatctt tttaaatgtt
     5041 tgggagtcac ttacggggga acacttagat tccaaaaagt ttattaaaaa tgaagaagta
     5101 agtgattctg aaggatatgt tatttctttc caagatggac cgcttaatta caatcatatt
     5161 attcatgaag tttatctaac gatgattaat acagctgaaa aatatattta tatttcaact
     5221 ccatacttaa tcttagatga tgaaatttta cttgctttaa aaatagcagc tgcaaggggt
     5281 attgatgtta gaatcataac acctaaaatg cctgataaga aatttgtatt tatggttaca
     5341 aaagaaaact ataaaccttt aattgaagct ggtgttagaa tttatgaata tacacctggc
     5401 tttatccata caaaagcagt agtgtctgat gatgatatcg ggactattgg aacatctaat
     5461 tttgattttc gcagttttta tttgcattat gaagatatta gtttaatcta taaaacaaag
     5521 agtgtaaaag atttacgaaa tgattttatt gaaacatttg caatttcaga gaaaattgaa
     5581 gcaaaagata taaagagaaa caatattttt aaaagagctc ttttagtagt tttaaagata
     5641 ttgtcaccat tactttaagg aggatattta tgaaagtaag gaaaatttta attggcttat
     5701 ttagtttaat ttttgtgttt tttgggacta tcattataaa tgctagacct gatgcagtac
     5761 cagaagatgt aactgtttca tctggagctt attattttgt agaggagaca agacaaacaa
     5821 atcatttacc ttatggtatt actcacaaaa gtgattgggc ttatagccat gagccattag
     5881 ctgatggcag tgtacctact atcgatagtt acttatcaca acaagtaaac atgttagaga
     5941 ttcctaagga tgctaatgtt tcgattgttg ggtgggcaaa tacagttgat aatcgttgga
     6001 cacttacgac agttaaaaga ttagcggaac aattcgaaag aaaatatcca gaatataaag
     6061 taataggggc aattaatggt gatggttttg atattagatc acaaggtgct tttccacgac
     6121 aacctcaaga cattactgct gggttaggta atttctttaa atcgcacttg ggagatgcta
     6181 atggtgtttt aggatttaga cgaaatggaa ctactgatac tttagtacat attcctaata
     6241 cagccgaaag agtcttaact ttagaagtaa ttgatgaaa
//
LOCUS       DAOSGS010000042         6245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11018_length_6245_cov_8.888691, whole genome shotgun sequence.
ACCESSION   DAOSGS010000042 DAOSGS010000000
VERSION     DAOSGS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11018_length_6245_cov_8.888691"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLQ22_02585"
     CDS             <1..493
                     /locus_tag="PLQ22_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58880.1"
                     /translation="HIFKYPPNENGERMYEVCGRKIGEKVTLQYAYETVKRFVDNYGE
                     RARKAAANEGIDWKAVSHAFRAAYQVKQILTENTITFPLKEAQTLLLIKQGKLDYQSV
                     VNPELNNLISEVEELSEKSNLPEKVDRNFWNNFIMDNLERYHFPNAKKYRELDNYIES
                     CLV"
     gene            968..2518
                     /locus_tag="PLQ22_02590"
     CDS             968..2518
                     /locus_tag="PLQ22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOH58881.1"
                     /translation="MATKSNVIKKKPPSYINGLKAITNWFFSNRRKKEELENIINIFK
                     IDNNIIKRFMLYTYNTPHLTWYINKYLNSLYEFNRFDTVNLLYSLVYLLDVNRITRKS
                     IPEKLMYLKNTELSDKNKQKVKELLYEFFDKIFDKNYNDSEMNYFYDLVNLNAITFKD
                     LEDIDKHINSGKSTLRLEEVTATTSPTSPKVNTSVLDIYKELPPGIKTFCNEAKEFIL
                     AREECKGCELFGKPTVIMDTNVIDSEEVDIAFIGLNPGTDEVEIGKPFVGKAGKILRE
                     RMSLLPNNVKWAIYNVILCHTKNENEIKNPDDVKARCKPLVEIIMQTFPSKIYVPLGA
                     KAFDWFGLKGSVASLAGKVFTNNNMTIVPVMHPSAANYNPENLAKFKSNFQSVLNLFN
                     QEQVKSPEVTIPQPPVKKSSKNYSKEDLTVPINNDKFIDKVTPDLTFFDVREINNKIL
                     KIYIDKNGQKKYLITDYELNFFLKNANWKQCDQITSGIDAVVTFPGRDKYSVIKKIRD
                     RLNNLKGA"
     gene            2524..2841
                     /locus_tag="PLQ22_02595"
     CDS             2524..2841
                     /locus_tag="PLQ22_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58882.1"
                     /translation="MWTFIQKDDVETFLNKYPHLAEGFEDIKLYETEDNIFLPRLFYK
                     NFPNGIKVFESRETPKFEPTFFKFIGKLRPEQIDIVQTAINMYNENHGQINGIIKARP
                     GLG"
     gene            3098..4153
                     /locus_tag="PLQ22_02600"
     CDS             3098..4153
                     /locus_tag="PLQ22_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH58883.1"
                     /translation="MKTVLSTFIAAQLGLKTCIVIDNENLMNQWVDAFLTFTDIKAEE
                     IGLIKGKHFVTDKPVIIATVQTLLSKIKTDMHKNFGSMDAARIGLVIYDEVHNTSSSS
                     KFAKASLLFRTKNFLGLSATPFQSGIAEILMKNTIGEIIYETKHYELKPTYVLNHYDS
                     GLTGKYAFVLGKMSDYIKRKAFYNSIIVRSSNYLNLIIKLIRKRLNEGHIILVLCFTK
                     AQVKLISERLDKEGIDHRRFYGDEKDEIDKNNVNVLIGTYAYIGKGFDFQQLSSLILA
                     TNLAGKKSLIQVVGRILREGDKNKKAPIVDDLIDRGFPSLFLPDMKSKKSVIKGEFNC
                     EIIDVVEDQEIQEGEII"
     gene            4210..>6245
                     /locus_tag="PLQ22_02605"
     CDS             4210..>6245
                     /locus_tag="PLQ22_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HOH58884.1"
                     /translation="MLFNKILGHCSFLGHTGYANHSRNFFTTLNNYLPVRIHNFSWWK
                     ELDHLDLVHHRMLIKQTWEEPPWEVGVPFYRAKSDKIISIVLMETNHYYYYDSYQDPV
                     IGYNVWEATKQPQKFFDRWNKFLQLWVPTEWQRQCTIEQGADPTKVKVVPEGVDGSKF
                     FPGEPELKLKEYKDGRFKFMIFGRWDYRKSTTEMIRTFIETFKRNEPVDLIISVDNPF
                     PADGLKSTEERLKHHKLLDPRIKILHFPDNQEYISHLKSGHCLLTCSRAEGWNLPLIE
                     GISCGTPTISSNYGAQLEFAENISHMVDIKDHISPREVFMDVHSSRNNNLEGTWAEPD
                     FEHLSKVMRDVYENYEEYKIKALVDSKIVREKFTWENAAKTALEHISEITEDDIEVHK
                     RIIKKSPSESVKYTFHNGARVDIFGSHDQEYTIKFIDNDTQKLVHQDTIETYHYVKTS
                     RMYYTNWKVIVEVNEELYSDHIFNPYGKRVIITLDSSALGDTISWMPYVEEFRKKHNC
                     KVFCSTLCNNLFKNGYPEIEFIPFGTTIDDIYAIYNVSMEEKDYNRNKTNWHLIPLQQ
                     AGSDFLGLDFKEIRPIIQVKKFKRAIKQKYVAIAEHSTFQCKYWNHPTGWQDLVKYLN
                     SVGYQVMPITREKSYLKNVVHKPNKELNDIVNNIQNAEFFIGVSSGLAWLAWGL"
BASE COUNT         2394 a          839 c         1061 g         1951 t
ORIGIN      
        1 acacatcttc aaatatccac caaatgaaaa tggtgaaaga atgtatgaag tttgtggtcg
       61 taaaattggt gaaaaagtaa ctcttcaata tgcatatgaa actgttaaaa gatttgttga
      121 caactatggt gaacgagcaa gaaaagctgc agctaatgaa ggaattgatt ggaaagctgt
      181 ctcacacgcg tttcgcgccg cctaccaagt taaacaaatt ttaacagaga atacaattac
      241 ttttccatta aaagaagctc agactctttt attaattaag caaggtaaat tagattacca
      301 atcagtagtt aatccagaac taaataatct aatttctgaa gtagaagaat tatcagaaaa
      361 aagtaatcta ccagaaaaag tagatagaaa tttctggaat aactttataa tggataatct
      421 tgaacgatac cattttccaa atgctaaaaa atatagagag ttagataatt acatagaaag
      481 ttgtttagtt taatttctct ccctgattgc gtttttcgtg tcgtttaaac cgcctccgat
      541 ctagatgctt tgaatctagc gacaaccata tccgtggtgg actcaatgtt ctatttataa
      601 cagggaataa atagacttgg gactcctttc tttaaaaaga gggccggtgg ttggccctca
      661 ttatttattc ttcaactttt atatagaaat attaataagg atggtaaaaa taatgataac
      721 ttcaacaatc ttaattggtg ctacaattat tttaactggt ataattattt ttcaatttta
      781 cttgttattc cgagcatttc aatggattta tttcattaat tcggtgctgt cagaactgtg
      841 taaaatatta ataaatcaca ctgataatat ctcaaaaaat actcaaataa atcaagttct
      901 caatgagttt ataaagaaag aaatgagcaa atcatcagat gtaacaataa attaaagagg
      961 atataatatg gcaacaaaat caaatgttat taagaaaaaa ccacctagct atattaatgg
     1021 attaaaagct atcacaaatt ggttctttag taatcgtaga aagaaagaag aattagaaaa
     1081 tattataaat atatttaaga ttgataataa tatcataaaa agattcatgt tatataccta
     1141 taatactcca catttaacat ggtatattaa taaatattta aattctctat atgagtttaa
     1201 tagattcgat actgtaaatt tattatatag tctagtttat ttactagatg ttaatagaat
     1261 taccagaaag agcattcctg aaaaattgat gtatcttaaa aatacagaat tgtcagataa
     1321 gaataagcag aaagttaaag agttattata tgaattcttt gataaaattt ttgataagaa
     1381 ttataatgac tcagaaatga attattttta tgatcttgtt aatttaaatg ctattacgtt
     1441 taaagatctt gaagatattg ataaacatat taatagcggc aagagcactc taaggttaga
     1501 agaagtgact gctactacgt ctccaacaag tcctaaggta aatactagtg ttcttgatat
     1561 ttataaagaa ttaccaccag gaataaaaac attttgcaat gaagctaaag aatttattct
     1621 tgcaagagag gaatgtaagg gatgtgaatt atttggcaag ccgacagtta taatggatac
     1681 gaatgtaatt gattctgaag aagtcgatat tgcatttatt ggtcttaatc caggaacaga
     1741 tgaagttgaa attggaaaac catttgttgg taaagctgga aaaatattaa gagaacgaat
     1801 gtctctgcta ccaaataacg ttaagtgggc aatctataac gtgattttat gtcatactaa
     1861 gaatgaaaat gaaatcaaaa atccagatga tgttaaagcg agatgtaaac cgcttgttga
     1921 aattattatg caaacctttc cgtcaaaaat ttatgttcca ctaggagcta aagcttttga
     1981 ttggtttgga cttaaaggat cagtagcatc attagctggt aaggtattta caaataacaa
     2041 tatgacaatt gttccagtaa tgcaccctag tgcagctaat tataatcctg aaaacttagc
     2101 aaaatttaaa agtaacttcc aatcagttct taatttattc aatcaagaac aagttaaatc
     2161 tccagaagtt actattcctc aacctccagt aaagaaaagt agtaagaatt attcaaaaga
     2221 agatttgaca gttccaataa ataatgataa atttattgat aaagtaactc cagatctaac
     2281 attttttgat gtgcgagaga taaataataa aattttaaag atctatatag ataagaatgg
     2341 acaaaagaaa tatttaataa ctgattatga attaaatttc ttcttaaaga atgcaaattg
     2401 gaaacaatgt gatcaaataa ctagtggaat tgacgctgtt gtaactttcc ctggaagaga
     2461 taaatattca gttataaaga agattcgtga tcgcttaaat aatctaaaag gagcataaga
     2521 acagtgtgga cattcataca aaaagatgat gttgaaacat ttttaaataa gtatcctcat
     2581 ctcgctgaag gttttgaaga tattaagtta tatgaaactg aagataatat atttctacca
     2641 agattatttt ataaaaattt tccaaatgga ataaaagttt tcgaaagtag agaaactcca
     2701 aaatttgaac cgacattttt caagtttata ggcaaactta gaccagaaca gattgatatt
     2761 gtccaaactg ccattaatat gtataatgag aatcatggac aaattaatgg aataatcaaa
     2821 gcaagaccag gactgggttg acaaactagc tcagtttaaa ctcctctaat tcggtgaaac
     2881 tcccactggg acaataccga gccaagcttc taataaagct gctaacttta ttagttgaag
     2941 gtgtaacgac tagtcgaaag acgtaagctg ataagcgatt gatcagctga aacggggagc
     3001 atcctaattt ctaggatgaa gatatagtct aatctcatag gtaactatga gcaggagtta
     3061 tcctgagtga aagagtagcg tcttacacta aatataaatg aagacggtcc taagcacgtt
     3121 tattgcggct caattaggac ttaagacctg tattgtaatc gataatgaaa atttgatgaa
     3181 tcaatgggta gacgctttcc ttacatttac agatattaaa gctgaagaaa ttggcttaat
     3241 taagggcaaa cactttgtca cggacaaacc tgtaataatt gcaactgttc aaactctatt
     3301 atctaaaatt aaaacagaca tgcataaaaa ttttggatca atggatgctg ctagaatcgg
     3361 attagttatt tatgatgaag ttcataacac aagttcttca agtaaatttg ctaaggcatc
     3421 tttactattt cgaacaaaga attttctagg actttcagct acaccatttc aatctggtat
     3481 tgctgaaatt ctaatgaaaa atacgatcgg tgaaatcata tacgaaacaa aacattatga
     3541 gctcaagccg acttatgtat taaatcatta tgatagtgga ttaaccggaa aatatgcatt
     3601 tgttcttggt aaaatgtcag attatatcaa gcgcaaagca ttttataatt ctattatcgt
     3661 aaggagttcg aattatttaa atttaataat taaattaatt cgaaaaaggc tcaatgaagg
     3721 acatattata ttagtattgt gttttactaa agcacaagtc aaattaattt cagaacgatt
     3781 agacaaagaa ggaatcgatc accgaagatt ttatggtgat gaaaaagatg aaatcgataa
     3841 aaataatgta aatgttctaa ttggaactta cgcttacatt ggaaaaggat ttgactttca
     3901 acaattaagt tctcttattt tagctacaaa cttagctggt aagaaatcct tgattcaagt
     3961 agttggaaga atattaagag aaggtgacaa gaataaaaaa gctccaattg ttgatgattt
     4021 aattgataga ggtttcccaa gtttatttct tcctgacatg aaatcaaaga aaagtgttat
     4081 taagggagaa tttaattgtg aaattattga tgttgttgaa gatcaagaaa ttcaagaagg
     4141 tgaaataatt tgattttatc ggcaagatct tatctaggtc ttgccgataa atatcttttc
     4201 ggaggcaaaa tgttatttaa taaaatattg ggacattgta gtttccttgg tcatactggt
     4261 tatgctaatc attcaagaaa tttctttaca actttaaata attatttacc agtaagaatt
     4321 cacaatttct catggtggaa ggaattggat catcttgatc tagtacacca cagaatgtta
     4381 attaaacaaa cttgggaaga accaccttgg gaagttggtg ttccgtttta tagagccaaa
     4441 agtgataaaa ttattagtat tgttttaatg gaaactaatc actattatta ttatgatagt
     4501 tatcaagatc cagtaatagg atataatgta tgggaagcta ctaagcaacc ccagaaattc
     4561 ttcgatcgat ggaacaagtt tttacagcta tgggtaccaa cagagtggca gcgacagtgt
     4621 acgatagaac aaggagctga tccaactaaa gtaaaagtag ttccagaagg agtagatggt
     4681 tctaaattct tccctggtga accagaacta aaattgaaag aatataaaga tggtcgcttt
     4741 aaatttatga tctttggaag atgggattat agaaaatcta caacagaaat gattagaaca
     4801 tttattgaaa cttttaaaag aaacgaacca gtagatttaa taatctcagt agataatccg
     4861 tttccagcag atggtctaaa atcaactgag gaaagattaa agcatcataa attattagac
     4921 ccacgaataa aaatattaca ttttccagac aatcaagagt atatttctca tcttaaatct
     4981 ggccattgtc tacttacatg ttcaagagct gaaggttgga atcttccgtt gatagaggga
     5041 atttcttgtg gaactcctac cattagctca aactatggtg ctcaattaga atttgcagaa
     5101 aatatttcgc acatggttga tattaaagat cacatatcac ctagagaagt attcatggat
     5161 gtacatagtt ccagaaacaa taatcttgaa ggaacttggg ctgaacctga tttcgaacat
     5221 ctcagtaaag taatgagaga tgtatatgaa aattatgaag aatataaaat aaaagctctt
     5281 gtcgattcta aaattgtaag agaaaaattc acatgggaga atgctgctaa aacagcacta
     5341 gaacatataa gtgaaattac agaagatgat attgaagttc ataaaagaat cattaaaaaa
     5401 tccccgtcag aatctgttaa atacactttt cataatggag caagggttga tatatttgga
     5461 tcacatgatc aagaatatac tatcaaattt atagataatg atacacaaaa attagtgcat
     5521 caagatacaa ttgaaacata tcattatgtc aaaactagta gaatgtatta tactaattgg
     5581 aaagtgatag tagaagttaa tgaagaatta tattctgatc atatatttaa tccatatgga
     5641 aaacgagtaa tcattacatt agatagctca gctcttggtg acactatatc atggatgcca
     5701 tatgttgaag aatttcgaaa gaaacataat tgtaaagttt tctgttctac tctctgtaat
     5761 aatctattta agaatggtta tccagagatt gaatttattc catttgggac aactattgat
     5821 gatatttatg caatatacaa tgtgtctatg gaagaaaaag actataacag aaataaaaca
     5881 aattggcatt taatcccttt acaacaagca ggatcagatt ttctaggttt agattttaag
     5941 gagattcggc cgattataca agtaaagaaa tttaaaagag caattaaaca aaaatatgtt
     6001 gctattgcag aacatagtac ttttcaatgt aagtactgga atcatccgac tggatggcaa
     6061 gatttagtta aatatttaaa ttcagttgga tatcaagtga tgcctataac aagagaaaaa
     6121 tcatatttaa aaaatgtagt tcataagcca aataaagagt taaatgatat agtaaataat
     6181 attcaaaacg ctgagttttt tatcggcgtg agctcaggat tagcatggct tgcttgggga
     6241 ttaca
//
LOCUS       DAOSGS010000043         6145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11251_length_6145_cov_4.509524, whole genome shotgun sequence.
ACCESSION   DAOSGS010000043 DAOSGS010000000
VERSION     DAOSGS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11251_length_6145_cov_4.509524"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="rsgA"
                     /locus_tag="PLQ22_02610"
     CDS             <1..395
                     /gene="rsgA"
                     /locus_tag="PLQ22_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH58885.1"
                     /translation="VLSGQSGVGKSTLLNTLGFNLKTQEISQALGRGKHTTRHVELYK
                     VLDGLVMDTPGFGTINLEEFNEIILAHSFKEFFYLSHNCRFATCLHINEPNCAVKEAL
                     EKGKILKSRYENYLLLHEEVKEILKKKY"
     gene            402..1049
                     /gene="rpe"
                     /locus_tag="PLQ22_02615"
     CDS             402..1049
                     /gene="rpe"
                     /locus_tag="PLQ22_02615"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOH58886.1"
                     /translation="MMKIAPSILSANFNHLYDDVKDLKKLGCDYLHVDVMDGHFVPNI
                     SFGTLAYENLKGKLDMIFDVHLMITNPLYYAPIFIKKGADILTFHLEVEQDKDEIIKV
                     IKENGAKAGISIKPKTNVEELVPYLDKIDLVLVMSVEPGFGGQKFMENSLPKIKFLDD
                     YRKKHKLNYLIEVDGGINEKTAKLCKDNGCDIAVCGTYVFKAKDRKKAIEAILNL"
     gene            1046..1636
                     /locus_tag="PLQ22_02620"
     CDS             1046..1636
                     /locus_tag="PLQ22_02620"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOH58887.1"
                     /translation="MICKIVGGSKDSFSKLYEKDENEYIIGLDSGTLLLNNLEIKVDL
                     AIGDFDSVDISDIKADKIIVYPKDKDYSDLELALMEIKDDKFSKVLVYNALEERFDHS
                     LSAIISLVKYFPLNICLFDLNNKIYLINKDTKFKKGDYKYISFFSQDAYITLSGFKYN
                     LDNYHLTSNDNLCLSNEIEEDAIIKTKDLVLVIESK"
     gene            complement(1669..1860)
                     /gene="rpmB"
                     /locus_tag="PLQ22_02625"
     CDS             complement(1669..1860)
                     /gene="rpmB"
                     /locus_tag="PLQ22_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH58888.1"
                     /translation="MARKCYVTGIGTQSGNNRPHSLKATRRKWKANLQKVRIKDADGK
                     IKRVYVSTRALRSGLVERA"
     gene            2235..3911
                     /locus_tag="PLQ22_02630"
     CDS             2235..3911
                     /locus_tag="PLQ22_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOH58889.1"
                     /translation="MSNTKILTGKLFKQIMNSGAKNLKANYKRIDELNVFPVPDGDTG
                     INMLMTIESGVKEMNAYTGDDLSGTMQKLSRGMLIGARGNSGVILSQIFRGLSNGIGD
                     AQEADAVLLASAYQEAVKQAYKAVMTPVEGTILTVARLAADHMSKVATKEMTIEQFYE
                     EYLNEAKKALDDTPNLLPVLKEAGVVDSGGAGYVTIVDGIVADLSGAVDVVVDLDASL
                     AATPTINLAFNSDGHSHENEFGYCTEFLLQLSDKYKNSVDERTILNEISVLGDSIVCV
                     KDGDIIKVHIHTLTPGVILNIGQRYGEFIQLKIENMTLQHNEIHHSEPKDKEPKIEVK
                     PNVKKEKYAIVTVATGDGLINTFKELGADYVIHGGQSMNPSTADFLNAYGAVNAENII
                     VFPNNSNIVLTAKQSAELYEDAKIWVLETKSIAQGFSAITMVDLEQEPELLLSNLKKV
                     IANVTTGQVTYSIRDADNNGIKIKKDDYIAICNGKIVASHKNRYETTKRLLKESITPD
                     KEIITIIRGKDADQREINNVVKHITKNYKNVEVDVVEGDQEVYSYILAIE"
     gene            3958..5985
                     /gene="recG"
                     /locus_tag="PLQ22_02635"
     CDS             3958..5985
                     /gene="recG"
                     /locus_tag="PLQ22_02635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOH58890.1"
                     /translation="MKNIPLTEKEYITKRNIAIFKRHKLNTVWDLLTYYPKRFNDYTI
                     VPTTEAPLEQTITISGIVQGKPFVYNARSKLTVMNFYIDSDGNKVRVTIFNRHFLRSK
                     LNYGVYVRVTGKFQKDIKNFTASEIELDEYSNNISPIFGIDGITDDKLFEIKQKLLRE
                     YRREIVDYLPQSLKDEHDLIDLDKAIKFVNTPDTMSQVEKAIKRIKFEELLLYQLKIK
                     YSLYMRKNHPEGLKVNFDQEKVNQFIKDLPFELTKDQKEAVDNILKDLKSDYKMSRLL
                     QGEVGSGKTVVAAIALYAVHTAGYQAAMLVPTEILAHQHFDTLKKAFKNTNLNLQLLT
                     ASVPLQEKKEILKDLEKGKVNIILGTHSLFQKDVKFHNLGFVVTDEEHRFGVKQRAEM
                     VAKGQKIDHLKMTATPIPRTLAISVMGDSDISTIKTMPGNKKPVITEYLQYKDKDLIM
                     THIKEELNRGRQVYVVCPAIEESESLDLKNVTNIYLEYKELFDGICEVGLIHSRLKQE
                     EKEEVMTKFKDGEIKILISTSVIEVGVNILNATTIVILDADRFGVAQLHQMRGRVRRS
                     DYQAYCFLVSDTNVETAIARLKLIASNSDGFTLAEEDLLVRGPGEYYGVKQSGIIDFQ
                     FANIINDRELLNLCNDEADRIINSGLIFTEDYKMLYNIVDEDFKTKSELLE"
     gene            5994..>6145
                     /locus_tag="PLQ22_02640"
     CDS             5994..>6145
                     /locus_tag="PLQ22_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH58891.1"
                     /translation="MVKLAIDAMGGDFAPEEIIKGVNLSIEEHKDLELILYGDEEQIK
                     KYLVPN"
BASE COUNT         2338 a          699 c         1093 g         2015 t
ORIGIN      
        1 ctgttttaag tggtcaaagt ggagttggta aatcaacatt acttaacact ttaggattta
       61 acttaaaaac acaagagatt agtcaagctt tgggaagagg aaaacatact acaagacacg
      121 tagagttgta taaagtctta gatggcttag taatggatac tccaggattt ggtacgatta
      181 atttagaaga gtttaatgaa ataattttag ctcactcatt taaagaattc ttttatttaa
      241 gccataactg tcgttttgct acttgtcttc atattaatga acctaattgt gcagtaaaag
      301 aagcattaga aaaaggaaag attttaaaat caagatatga aaactatctt ttattacatg
      361 aagaagttaa agaaatatta aaaaagaagt attaggagga tattatgaaa atagcaccat
      421 caattttatc agcaaacttc aatcatttat atgatgatgt gaaagattta aagaaattag
      481 gatgtgatta tctccatgta gatgtaatgg atggtcattt tgttcctaat attagttttg
      541 gaactttagc ttatgagaat ttaaaaggga aattagatat gattttcgat gttcatttaa
      601 tgattactaa tcctttgtat tatgctccta tatttatcaa aaaaggtgct gatattttaa
      661 cttttcattt agaagtagaa caagataaag atgaaataat taaagttatt aaagaaaatg
      721 gagctaaagc tggaatttca attaaaccga agactaatgt ggaagagttg gttccttatt
      781 tagataaaat tgatttagtt ttagtaatga gcgttgaacc tggctttggt ggccaaaagt
      841 ttatggaaaa tagtttacca aaaatcaagt ttttagatga ttatagaaag aaacataaac
      901 taaactatct aattgaagta gatggcggta taaatgaaaa aacagccaaa ctatgcaaag
      961 ataatggctg tgatattgcg gtgtgtggga catatgtgtt taaagcaaaa gaccgtaaga
     1021 aagcaattga ggctattttg aacttatgat ttgtaagatt gtaggtggat ctaaagattc
     1081 ttttagtaaa ctatatgaaa aggatgagaa tgaatacatt attggcttag atagtgggac
     1141 acttttactt aacaacttag aaattaaagt agatctagct ataggtgact ttgattcagt
     1201 agatattagt gatattaaag ctgataaaat aattgtttat cctaaagata aagattatag
     1261 tgatttagaa cttgctttaa tggaaataaa agatgataaa ttttctaaag tgttagttta
     1321 taatgcttta gaagaaagat ttgaccattc tttatcagct attattagtt tagttaagta
     1381 ttttccatta aatatttgtt tatttgattt aaataataag atttatttaa taaataaaga
     1441 tactaaattt aaaaaaggag attataagta tatttcattt tttagtcaag atgcttatat
     1501 tactttaagt ggatttaagt ataatttaga taattatcac ttaactagta acgataactt
     1561 atgtttaagt aatgaaattg aagaagatgc tattataaag actaaggatt tagttttagt
     1621 tattgaaagc aaataaaaaa ggcttatatt ctaagccttt tatttacatt aagctctttc
     1681 tacaagtcca gatcttaatg cacgtgttga tacataaaca cgtttaattt tgccgtcagc
     1741 atctttaatt cttacttttt gtaagttagc tttccacttt cttctagtag cttttaaaga
     1801 gtgaggacgg ttgttaccag attgagtacc gattccagta acataacatt tacgagccat
     1861 aactaaaacc tccttactac tcttaaatac tttgatatta tatcaaaaag tttaagaaaa
     1921 agcaacttat ttgtgaaaga aaatttgtat aaaaaagagt taaaatttgc attttatggt
     1981 aagaaaatgg caaaaacagc tcaaaaacag cgaaaaaggc ctgaatttgg cgtttctgag
     2041 cgttttatct tgttttaggt aatttgacct tcattaaaac tacgagagca agcaaaaaag
     2101 acctctcaat gcttgttttt aaggtaaaac taaagaattg taaagataaa gtaagtttta
     2161 aggtattttt aagggttgca atatataata cttaaaaata aaagtatatg tataaataag
     2221 gagaggtgtt taaaatgagt aatacgaaaa ttttaacggg caaattattc aaacaaatta
     2281 tgaatagtgg cgcgaaaaat ttaaaagcta attacaaaag aatagacgaa ttaaacgtat
     2341 tccctgtacc cgatggtgat acaggtataa acatgcttat gacaattgaa agtggcgtta
     2401 aagaaatgaa cgcatataca ggtgacgatt taagcggtac aatgcaaaaa ttatctcgtg
     2461 gaatgttaat tggagcacgt ggtaattctg gagttatctt atcgcaaatc ttccgtggtt
     2521 taagtaatgg tatcggtgat gctcaagaag ctgatgcagt attacttgct agcgcttatc
     2581 aagaagctgt aaaacaagca tataaagcag ttatgacacc tgtagaggga acaattttaa
     2641 ctgtagcacg tttagcagct gatcatatga gcaaagttgc tacaaaagag atgacaattg
     2701 aacaatttta tgaagaatat ttaaatgaag ctaagaaagc tttagatgat acacctaatt
     2761 tactaccagt attaaaagaa gctggagttg tagatagtgg tggtgctggt tatgtaacta
     2821 tcgtagatgg tatcgtagct gatttatcag gtgctgttga tgttgtagtt gatttagatg
     2881 catcattagc tgctacacct actatcaatc tcgcttttaa tagtgatggt catagtcatg
     2941 agaatgaatt tggttattgc acagaattct tacttcaatt atctgataaa tacaaaaata
     3001 gtgttgatga aagaacaatt cttaatgaaa ttagtgtttt aggtgatagc atcgtttgtg
     3061 ttaaagatgg agatattatt aaagttcata ttcatacttt aactcctggt gttattctaa
     3121 acattggtca aagatatggt gaatttattc aattaaagat tgaaaatatg actttacaac
     3181 ataacgaaat ccatcatagt gaaccaaaag ataaagaacc taaaatagaa gtaaaaccta
     3241 atgttaagaa agaaaaatat gctattgtaa cggtagctac tggcgacgga ctaattaata
     3301 cttttaaaga actaggagca gattatgtta tccatggtgg ccaaagtatg aatccatcaa
     3361 cagctgattt cttaaatgct tatggtgccg taaacgcaga aaatatcatt gttttcccta
     3421 ataatagtaa tatcgtatta actgcaaaac aatctgcaga gttatacgaa gatgctaaaa
     3481 tctgggtatt agaaactaaa tcaattgctc aaggattctc agcaatcaca atggtagatt
     3541 tagaacaaga acctgaatta ttgttaagca acttaaagaa agtaattgct aatgtaacta
     3601 caggacaagt tacttattca attagagatg ctgataacaa tggtatcaaa attaaaaaag
     3661 atgattacat agctatttgt aatggtaaaa ttgtagctag tcataagaat cgttatgaaa
     3721 ctactaagag actattaaaa gaaagtatca ctcctgataa agaaatcatt actattatca
     3781 gaggtaaaga tgctgatcaa agagaaatta ataatgttgt taaacacatt acaaagaact
     3841 ataaaaatgt ggaagtagac gttgttgaag gagatcaaga agtctatagt tatattctag
     3901 cgatagaata atcaaggggg cttaaagccc ctttaattat aatttaggag gaataatttg
     3961 aaaaatatcc cattaactga aaaagaatat attacaaaaa gaaatattgc tattttcaaa
     4021 agacataaac ttaatactgt atgggatcta ttgacttatt atcctaaaag atttaacgat
     4081 tatacgatag tcccaacaac tgaagctcca ctagagcaaa ctattacgat ttctggtatt
     4141 gttcagggga aaccttttgt ttataatgcc agaagtaaat taacagttat gaatttttat
     4201 attgatagtg atggtaataa agtaagagtt actattttta atcgtcattt tttacgttca
     4261 aaattaaatt atggcgttta tgttcgtgta actggtaagt ttcaaaaaga tattaaaaac
     4321 tttacagcta gtgaaattga acttgatgaa tatagtaata acattagtcc catttttgga
     4381 attgatggta ttactgatga taaattattt gaaatcaaac agaaattatt aagggaatat
     4441 cgaagagaaa ttgttgatta tttacctcaa agtttgaaag acgagcacga tttaattgat
     4501 ttagataaag cgattaagtt tgtgaatacg cctgatacaa tgagtcaagt tgaaaaagca
     4561 attaaaagaa tcaaatttga agaattattg ctttatcaat taaaaattaa gtattcacta
     4621 tatatgagaa agaatcatcc tgaaggttta aaagtaaatt ttgatcaaga aaaagttaat
     4681 cagtttatta aagatttacc ttttgaactt actaaagatc aaaaagaagc tgtagataat
     4741 atactaaaag atttaaaatc agattataaa atgagtagat tattacaagg tgaggtaggt
     4801 tctggtaaaa ctgttgtagc tgcgattgct ttgtatgcag tgcatactgc aggctatcaa
     4861 gctgcaatgt tagttccaac tgaaatttta gctcatcaac attttgacac tttgaaaaaa
     4921 gcttttaaaa atactaattt aaacttacaa ttattaactg ctagtgtacc attacaagag
     4981 aaaaaagaaa tattaaaaga tttggaaaag ggaaaagtaa atattatact tggaacacat
     5041 tccctatttc aaaaagatgt taagtttcat aatttagggt ttgttgttac agatgaggaa
     5101 catcgttttg gtgtaaaaca aagagcagaa atggtagcta aaggtcaaaa gattgatcat
     5161 ttaaagatga cagctacacc aatcccaaga acattagcga tttctgttat gggagattct
     5221 gacatctcta caattaaaac aatgccagga aataaaaaac cagtaattac tgagtattta
     5281 caatataaag ataaagatct tattatgact catattaaag aggaattaaa tcgtggaaga
     5341 caagtttatg ttgtttgtcc agcaattgaa gaatctgaaa gtttagattt aaagaatgtt
     5401 acaaatattt acttagaata taaagaatta tttgatggta tttgtgaagt aggattaatt
     5461 catagtcgtc taaaacaaga agaaaaagaa gaagtaatga ctaaatttaa agatggagag
     5521 attaaaattc ttatctctac tagtgttatt gaagtaggag ttaatatttt aaatgcaact
     5581 acaattgtta ttttagatgc tgatcgtttt ggtgtagctc aacttcacca aatgcgtggt
     5641 cgtgttagaa gatcagacta tcaagcatat tgttttctag tatctgatac gaatgttgag
     5701 acggcaattg caaggttaaa attaattgct tctaactctg atggttttac tttagctgaa
     5761 gaagacttgc ttgtaagggg accaggagag tattatggtg ttaagcaaag tggaattatt
     5821 gattttcagt ttgccaatat cattaatgat cgtgaattat taaatttatg taatgatgaa
     5881 gccgatagaa ttattaattc tggtttaatc tttacagaag attataagat gttatataat
     5941 attgtagatg aagatttcaa aacaaaaagt gaacttttag aataggagga actatggtaa
     6001 aacttgcaat tgatgctatg ggtggcgatt tcgctcctga ggaaataatt aaaggtgtta
     6061 atttaagtat tgaagagcat aaagatttag agttaatttt atatggcgat gaagaacaaa
     6121 taaagaaata tttggttcct aataa
//
LOCUS       DAOSGS010000044         6128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11286_length_6128_cov_4.109830, whole genome shotgun sequence.
ACCESSION   DAOSGS010000044 DAOSGS010000000
VERSION     DAOSGS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11286_length_6128_cov_4.109830"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLQ22_02645"
     CDS             complement(<1..319)
                     /locus_tag="PLQ22_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58892.1"
                     /translation="MKRLFKFILLFVLGLFVTLLAIPKVKGFGNITYEHIITTVGEDP
                     ATSVRINWHSEKLNTYLEYTLASDTVYANATTVTPETHMWSLPNEETINGVTYNHEGF
                     NRQY"
     gene            complement(438..1109)
                     /locus_tag="PLQ22_02650"
                     /pseudo
     CDS             complement(438..1109)
                     /locus_tag="PLQ22_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            1825..2949
                     /locus_tag="PLQ22_02655"
     CDS             1825..2949
                     /locus_tag="PLQ22_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOH58893.1"
                     /translation="MKVLGVILELNPPHNAHKYFIEQAKMQIKPNLTIAIISCNFAQR
                     GEISIISKYEKTKAALNLGADLVIELPVFATLNSAELFGYYAVKILNDFKITDLAFGV
                     ELDDLNLLKKTNQILESPSYNEKLKANLNHGYSYKKAALLAFEKSIDNPILTENFNKP
                     NNTLALKYLSAIDKINPKLQVNLIKRIGENEYSTNIGEYPSGSTIRNLISKGKDVSKF
                     IPENYNFINYNQAYDNLFYLFKSFVLLEENPFKNTKHIKEGIENRIYKVLKETFNYKD
                     FISKMKTKRYQENYLQRLLLSVLLELENQDDYYLRVLGFNKNGEKHLSNIDKSIITSP
                     KNINNKLLNIELKASKLYALITNNDNYYQEEFLIPYKENK"
     gene            2949..3947
                     /locus_tag="PLQ22_02660"
     CDS             2949..3947
                     /locus_tag="PLQ22_02660"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOH58894.1"
                     /translation="MNTKDIRLKLEGIIDPSVNKTLKETDGIKHIGIDSDKDIAILII
                     TMEKKDSESEKTLKREIAKAIKLDLGFKGVKIQLEERRINESIVKTNAKFLIITSGKG
                     GVGKSTVAANLAYYLKKNGKTVALLDADIYGSSLPRVLDMEEGYPGVNEKGKLIPRKV
                     KDMEIMSTEFFVEGNKPVIWRGAMLHSMMNNFFYETSWDKNLDYMVIDAPPGTGDVAL
                     DLRQIVPNAKSIVVTTPHKTASHVAIKAGYASVKLKQEVLGVVENMAYYYNEYSKEKD
                     YIFGEGGGKEVAENLNVELLAQIPINRPKHNVGLYEEDEKAGQIFNDLAKKIIEIY"
     gene            complement(4002..4508)
                     /locus_tag="PLQ22_02665"
     CDS             complement(4002..4508)
                     /locus_tag="PLQ22_02665"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOH58895.1"
                     /translation="MPKEMENKLVIQDAEGNEILCEILFTHYSEKFKKNYVLFYEVAD
                     EDNEEIEVMAASYEEGKEGDGQLDPIESDEEWVYIEDLLEAFSSEDEDDECECDCEEG
                     EECECGCQEHQHQHKHKKGECKDGEECECQEHQHRKGDAVCQEKHQKGECQDRDRNRN
                     RDRKKCNK"
     gene            complement(4531..4941)
                     /gene="ruvX"
                     /locus_tag="PLQ22_02670"
     CDS             complement(4531..4941)
                     /gene="ruvX"
                     /locus_tag="PLQ22_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH58896.1"
                     /translation="MRAIGIDLGSKTVGISLSDINRIIASPIGTFEIEENNLNEALEL
                     VVDYIKKNNVSTIVLGLPKNMDGSIGFQGEYCLEFKKMLEDATKLEVVLIDERLTTVI
                     AHKTLKEAKMKNQKRKQKVDKLAASIILQAYLDK"
     gene            complement(4938..>6128)
                     /locus_tag="PLQ22_02675"
     CDS             complement(4938..>6128)
                     /locus_tag="PLQ22_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH58897.1"
                     /translation="DQGKLIFQDQEYEVKDTIVLPNDQHASLVDFGGDYIKVADKVIL
                     QVDKALRFNTAKNHSATHLLNYALKEVLGSHVHQQGSSVSSNSLRFDFNNFSLPTDEE
                     LLKVEAIVNRMIDLGHHVETKEMALDKAKTLGVEAMFSDKYEDVVRVVTIGDSKELCG
                     GTHVSNAKEIGKFVILSCETKGSGIYRIEATTDTNIKDKLLESADNILIEISNLKQKA
                     EELVKSAKEEKLDIVYKAPKDFKYLESYKYLLDLKELSTKYQVLVKDIDKEYQKQYRE
                     SKQISPALFKDKVEVINGVKVVIDRLEDVDSEVLKDLVDNLSDNLQLEIVLLANVSSG
                     RIVYVSKNKNKKVKAGDLVKMAAIVTGGNGGGRPDFAQAGGRDVTKVDESLAEVRQYL
                     KEVL"
BASE COUNT         2121 a         1105 c          748 g         2154 t
ORIGIN      
        1 aatactgacg attaaaacct tcatggttat aagtaactcc attaattgtt tcttcattag
       61 gtaaactcca catgtgcgtt tctggtgtta cagtagtagc attagcataa acagtatcac
      121 ttgctagtgt atactctaaa taagtattta acttttctga atgccagtta atacgaactg
      181 atgttgctgg atcttcacct acagtagtaa taatatgctc ataagtaata ttaccaaatc
      241 ctttaacttt gggaattgca agtaaagtta caaatagtcc caaaacgaat aataaaataa
      301 atttaaataa tcttttcatc tttttctcct tcttctatat aatatgttgg ttttaataat
      361 gtataaataa atgaataatc actatttgat ttaaataatg gttttaaatt atcctttgtt
      421 ccccaaattg aataaacaaa ctctggatga tctacgaaag gatttctatt ttcttgataa
      481 ctataaataa catcatttct ctttctttca aaatcatcta ctgggtcttc ctcatgccat
      541 tttaataaag ttgatagttt ccctaaatat ggcgtgctac catttccaac cttatcattt
      601 aattctaagt cagttggaca accatcacca ccatcataac gtgttgccat ataaaacatc
      661 attcttgcaa tatcaccttt tacttcatca cgaggttcaa aactaacacc agtaatacga
      721 taacaaaaac tagaaccttc accataacca agagctgatt caactttaac tccaccttca
      781 tcaaaatcta agttacctcg atcagagttt acattgacaa ctgtcggtct taaatgatgc
      841 atatcagttc cggcactcgg actatcgcca aaacctccat gactcttagg ccaagtatgt
      901 tcacgattcc aagcttgagt tgatccgttt ttaggataac taactcctgt atacattaaa
      961 atcatattat caggattatt tggatcttca tctgtttccc ttaaataagt tcttagacta
     1021 ctataactat aagtagtatg accagaaata atcgaatgta aagctaactt taactctcgt
     1081 ccagttttcc ctgatgctgc ctcataatat cccgcataat catcaggaac atactctact
     1141 tttaaaactt taacttgata agttttaatt tcttctaaag ttttatatgt aaaggtagca
     1201 gttaaagtaa ctgttttatc cgtacttcct cgagccacta caccctgatt agtaattaca
     1261 tcttcattat cagactgcca agaaataaca acttcttgat aattagtagc taaatcaaga
     1321 tcacttatag cttcaggcgg taaactaata ctagcaatag cttctgccat taaaacttca
     1381 tcttgattat cttcaccatt tgaatctttt tccttatgaa taacagtaat tttaaactct
     1441 ttacttctag tctttacccc atcagtaata taggcatata aagtaagact aatgtctacc
     1501 tcttgcctaa aaacatagcc taattccgta actactttag tattagaaga ttgccagctc
     1561 acttgataac ccaaaacagc ttttggtaaa tccaagtcct ctacaacact atttaaatct
     1621 ccttcaatag ttaattcatt atacgctttt tcaaatggat cttctttttc cacacaccct
     1681 acaaataaaa aaattgtaag tattgaaata aagaagataa atacttttct catatcaatc
     1741 acctatatat attattatac catatttctc gtcaaattta tagaattaaa tacccaattg
     1801 tgctataata aataaggtga tttgatgaag gtcttaggtg ttattttaga attaaatcct
     1861 cctcacaatg ctcataaata ctttattgaa caggcaaaga tgcaaataaa acccaactta
     1921 acaattgcaa ttatctcatg caacttcgct caaagaggag aaataagtat aatcagtaaa
     1981 tatgaaaaaa caaaagctgc acttaactta ggtgctgatt tagttataga gttgccagta
     2041 tttgctactt taaactcagc tgaattattt ggctattacg cagtaaaaat cttaaatgat
     2101 tttaaaatta cggaccttgc ttttggtgtt gaattagatg atttaaactt acttaaaaaa
     2161 actaatcaaa ttttagaaag cccaagttat aatgaaaaac taaaagctaa tctaaatcat
     2221 ggctatagtt ataaaaaagc agctttactt gcttttgaga aaagtataga taatcctatt
     2281 ttaactgaaa actttaataa accaaacaat actttagcct taaaatacct aagtgctatt
     2341 gataaaataa accctaaact tcaagtcaat ctaattaaaa gaattggaga aaatgaatat
     2401 agcacaaata ttggtgaata tccatcaggc tctacaatta gaaatttaat ctctaaaggt
     2461 aaagatgttt ctaaatttat ccctgaaaac tataatttca ttaattataa ccaagcctat
     2521 gataatctat tctatttatt taaatcattt gtacttttag aagaaaatcc gttcaaaaac
     2581 acaaaacaca ttaaagaagg aatagaaaat agaatctata aagtcctaaa agaaactttt
     2641 aattataaag acttcatctc aaaaatgaaa actaagagat atcaagaaaa ctatcttcaa
     2701 agactactct tatcagttct cttagaactt gaaaatcaag atgattacta tttaagagtt
     2761 ttaggtttta ataaaaatgg tgaaaaacat ttatcaaata ttgataaatc tataattaca
     2821 agccctaaaa atataaataa caaattacta aatattgaac ttaaagcaag taaactctat
     2881 gctttaatca caaacaatga caattattac caagaagaat ttttaatacc ttataaggag
     2941 aataaataat gaatacaaaa gatattagac taaaactaga aggaattatt gatccttcag
     3001 taaataaaac cctaaaagaa actgacggta tcaaacatat cggcattgac tctgataaag
     3061 atatagctat cttaatcatc acaatggaga aaaaagatag tgagtcagaa aaaaccctaa
     3121 agcgagaaat agctaaagca attaaactag atcttggttt taaaggagtt aaaatccaac
     3181 ttgaagaaag aagaattaat gaatcgattg taaagacaaa tgctaagttt ttaattatca
     3241 catctggtaa aggtggtgta ggtaaatcca ctgttgctgc taaccttgct tattacttaa
     3301 agaaaaacgg taaaactgtt gccttacttg atgctgatat ttatggttct tctttaccta
     3361 gagttcttga tatggaagaa ggctatccag gagtaaatga aaaaggaaaa ctaattccaa
     3421 gaaaagtaaa agatatggaa attatgtcaa ctgagttttt tgtagaggga aataaacctg
     3481 taatttggcg tggcgctatg cttcactcaa tgatgaataa cttcttctat gaaacttcat
     3541 gggacaaaaa cttagactat atggttattg atgccccacc tggaactggc gatgttgcct
     3601 tagacttaag acaaattgtt cctaacgcta aatcaattgt agttacaaca cctcataaaa
     3661 ctgcaagcca tgtagctatt aaagcaggct atgcttcagt taaactaaaa caagaagttt
     3721 taggggttgt agaaaacatg gcttattact ataatgaata ttcaaaagaa aaagactaca
     3781 tctttggtga aggtggagga aaagaagttg ctgaaaactt aaatgtagaa cttttagctc
     3841 aaatcccaat caataggcca aagcataatg taggactata tgaagaagat gaaaaagcag
     3901 gtcaaatctt taatgacctt gctaagaaaa taattgaaat ttattaattt ataatatttt
     3961 accaaataaa aaagctcaaa taattgagct ttttttaata attatttgtt gcactttttg
     4021 cgatcacgat tgcggttacg atcacgatct tgacattcac ccttttggtg tttttcttgg
     4081 catacggcat cacctttacg atgttgatgt tcttggcatt cgcactcctc accatctttg
     4141 cattcgcctt ttttgtgttt atgttggtgt tgatgttctt gacatccaca ttcacattct
     4201 tcaccttctt cgcaatcaca ttcacactca tcatcttcat cttctgaaga aaatgcttct
     4261 agtaaatctt caatatatac ccattcttca tcactttcaa ttggatctaa ttgtccatct
     4321 ccttcttttc cttcttcgta agaagcagcc attacttcaa tttcttcgtt gtcttcatct
     4381 gcaacttcat aaaataacac atagtttttc ttaaacttct cactgtagtg tgtaaataaa
     4441 atttcacata aaatttcatt tccttctgca tcttgaatta ctaatttgtt ttccatttct
     4501 tttggcataa tctaattttc ctttctcctt ttatttatcc aaataagctt gcaagataat
     4561 acttgcagct aatttatcaa ctttttgttt tcttttttgg tttttcattt tagcttcttt
     4621 tagagtttta tgagcaataa ctgtcgttag tctttcatca attaaaacaa cttctaactt
     4681 agttgcatct tctaacatct ttttaaactc taaacaatac tctccttgaa aaccaatact
     4741 tccatccata tttttaggta atcctaaaac tatcgtacta acattgtttt tctttatata
     4801 atctactact aattctaaag cttcatttaa attgttttct tcaatttcaa atgttccaat
     4861 aggacttgca ataattctat ttatatcaga taaactaata cctacagttt tactacctaa
     4921 atcaataccg attgctctca tagtacctct tttaagtatt gtctaacttc cgctaaagat
     4981 tcatcaactt ttgtaacgtc tcttccacca gcttgtgcaa agtctggtct accaccacca
     5041 ttaccaccag taacaattgc agccatcttt actaaatcac cagctttaac ttttttgttt
     5101 ttgtttttcg aaacataaac aattcttcca ctacttacat ttgctagtaa tacaatctct
     5161 aattgtaagt tatcacttaa attatcaact aagtctttta gaacctcact atcaacatct
     5221 tctagtctat caataacaac tttaactcca ttaattactt ctactttatc tttaaataaa
     5281 gctggactaa tttgttttga ctcacgatat tgtttttgat attctttatc aatatccttt
     5341 acaagtactt gatacttagt agatagttct tttaaatcaa gcaaatactt ataactttct
     5401 aaatacttaa aatcctttgg agctttataa actatatcta acttttcttc tttagcgctt
     5461 tttactaatt cttcagcttt ttgttttaag ttactaatct ctattagtat attatccgca
     5521 ctttctaata atttatcttt tatgtttgta tctgtagttg cttcaattct gtaaatacca
     5581 gaacctttag tctcacaact taaaatcaca aactttccta tttccttagc attacttaca
     5641 tgagttccac cacataattc tttagaatca ccaatagtaa caactctaac aacatcttca
     5701 tatttatcag aaaacattgc ttctacccct aaagttttag ctttatctaa agccatctct
     5761 ttagtttcaa catgatgacc taaatcaatc atcctattta caatagcttc tacttttaaa
     5821 agttcttcat ctgttggtaa gctaaagtta ttaaagtcaa atcttaaact attacttgat
     5881 actgatgagc cttgttggtg aacatgactt cctaaaactt cttttaatgc atagtttaaa
     5941 agatgggttg cagagtggtt tttagctgtg ttaaatctta aagctttatc aacttgtaag
     6001 ataactttat cagctacttt gatataatct ccaccaaaat caactagcga tgcatgttgg
     6061 tcattaggta aaacaattgt atcttttact tcatattctt ggtcttggaa gattaattta
     6121 ccttgatc
//
LOCUS       DAOSGS010000045         6112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11342_length_6112_cov_8.962523, whole genome shotgun sequence.
ACCESSION   DAOSGS010000045 DAOSGS010000000
VERSION     DAOSGS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11342_length_6112_cov_8.962523"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="PLQ22_02680"
     CDS             complement(<1..1958)
                     /locus_tag="PLQ22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58898.1"
                     /translation="MQTRLSDKERKRLGFINRQPSLSRHSAPVVLIEPTDDSEDETIH
                     VSPSFLEPLNADLDGDTVALYIIHDREALLECEDKAFLQNSITYDQNNSFLATIRHEA
                     LYAAFVLTKEKFNKDNIIIKNLRSLKDLPESIKLWNDHLYDTVQMRSGELYSYGRCLF
                     NKFCGFDKVIITKSLSKKLIDSVSEELYNYYNKDNKKYYDSLAALEKQLFFFISSTSH
                     APSLDVENMVNLKNDQTNKIFKNLPNNNIKLGYYIVEALTNRALDNMDENSSLYKLYK
                     SGSRFSKAQLARSAVAIGYSADADNVVIPRPIKTSLLEGLSEEQYFMVAPATRKSIKD
                     KSRHTPNSGYLERTLVMALSMIEFDLDDCETNNHLEFIVMSNKHAQTLVGKYHRDPNS
                     TDREWKVLDLKTAIGFINRKIEIRSPMTCQNPNFKICKKCFGEKELKTKYVGIVAGQC
                     VVERLTQLTLRTFHESGRANLPTDVEILKFFEDHLVDVETDESGTTLIFDNDSFPEKL
                     ITNQNPLIYGLTQVSNNKLIFTQDKSSIKNQDVIAVVNTIKNILKQGDVFQPIEDYYS
                     NLMAAILEVGTIYSSFIEILLANMFVVDYNNKLFWRYHQDQTPTYKLGDKGLAAYISS
                     RIGLLYQPNKNTIENISLDELEEIDQKN"
     gene            complement(1969..3195)
                     /locus_tag="PLQ22_02685"
     CDS             complement(1969..3195)
                     /locus_tag="PLQ22_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58899.1"
                     /translation="MTECLGGEVTSIKFYTGKYSNVDENGLLSESIFGPIHDFRCRCG
                     KLNIESIDGGKVCDRCGVLCGPSDLRLRTFGKITLVFPVIKSTKRKYFKKIVGSQHKH
                     LLDPKKADALAATSRYLAVTHTADTLKVVNTMNFVQGWFVVPIRITGLYSFILSLKFV
                     AEVLGLDIAKELFEKKYIIDVVKILPPDIRPVVRDPKKRSELRYVEINKHYISLINQN
                     NQNKPMKDIVRQKESDWFDKIRYNLNNEIVDEIVDVAIPEYDAATARCQYYVDKIYES
                     IFEGISGKTGLIRSSILGKTIEFSARSVIVIDPSLESYKIKVSKKILYRLWFPYFLYY
                     LSKFKDYEFYDIFDIHTQERNFEDNKEIFDEFLNWFCQPESDEKNIDDEFEMSSVKSL
                     IRRRKIEQEMLEDAFD"
     gene            complement(3210..3650)
                     /locus_tag="PLQ22_02690"
     CDS             complement(3210..3650)
                     /locus_tag="PLQ22_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58900.1"
                     /translation="MFRLPEDAELEIQKKFPNAPIQQITQYLFQLILHKTLNDGACPV
                     RGFGKFVSFKTRSNRVGQDVIRFKFKISNALINKLKNDQYMLNNIPVKAVNVFAEMHE
                     NKTKDKKPQSKANIDASKEAEKLGKKKTEERIVLNIINDMISKD"
     gene            complement(3665..4111)
                     /locus_tag="PLQ22_02695"
     CDS             complement(3665..4111)
                     /locus_tag="PLQ22_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58901.1"
                     /translation="MSKKNKLDEGFSTLQDDSLTVQENSSEEQISETPESFEISPQEV
                     SKNIFILAKFSDAIVELGYVFLATNEQNVVTASNGRINLLQGRMYYIPIDKSNIDSDV
                     FNIKIHSDVADRIDVRFVKDGFACVVPIRHNVTLKTGEKLCELIPF"
     gene            complement(4117..4515)
                     /locus_tag="PLQ22_02700"
     CDS             complement(4117..4515)
                     /locus_tag="PLQ22_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58902.1"
                     /translation="MSLTLGYFSKFPAAYKDREKSIGEVRFNNIHQDIIKDKIIIHAS
                     TDKYQQEMEIQDEIIHLNSPVKVFCTCESFKYEFANAVFRAGSLLKSINFVRSIISRP
                     KEKNQYNVPSGCKHIVALARQVIKIKPKRG"
     gene            complement(5499..6053)
                     /locus_tag="PLQ22_02705"
     CDS             complement(5499..6053)
                     /locus_tag="PLQ22_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58903.1"
                     /translation="MNRKDLKKMGEHEKTTKSLLAIDQKTKHLWLQGETSVLYAPASQ
                     ITEIRIIQTVQKLFNIYAKVNLIYRMEDHLLEVKKSASAAKKFVEDFVKDLQKEEYVK
                     NLKGTTFIKKKFILGFSVVGTDLVALIVNSPVPVIMASYTSEEDAIEALEHFVTTGTY
                     KTEKIKLDETGLKALDLLVEEISK"
BASE COUNT         1923 a         1034 c          771 g         2384 t
ORIGIN      
        1 aaatttttct gatcaatttc ttccaattca tctagactaa tattttctat tgtattttta
       61 tttggttgat ataacaatcc aattctacta gaaatatatg cagccaatcc cttatcacca
      121 agtttataag ttggggtttg atcttgatga tatctccaaa ataatttatt attataatca
      181 accacaaaca tattagcaag taaaatctca ataaatgaac tgtaaatagt tccaacttcg
      241 agaattgctg ccattaaatt agaataataa tcttcaattg gttgaaatac atcaccctgt
      301 tttagaatat ttttaattgt attaactaca gcaattacat cttgattctt tattgatgat
      361 ttgtcttgtg taaatattaa tttattgtta ctaacttgag tcagaccata aattaatgga
      421 ttttgatttg ttattaattt ctccggaaaa gaatcattat caaaaattaa tgtagttcca
      481 gattcatctg tttcaacatc tactaaatgg tcttcaaaga attttaatat ttccacatct
      541 gttggtagat tggctcttcc tgattcgtga aacgtcctga gcgtaagttg agtcagacgc
      601 tctactacac actgtccagc aactatacca acatacttcg ttttaagttc tttttcacca
      661 aagcattttt tgcatatttt aaaatttggg ttttggcaag tcataggaga tcgaatttca
      721 atttttctat tgataaatcc aatagcggtt tttaaatcaa gaactttcca ttctctatct
      781 gttgaatttg gatctctgtg atattttcct actaatgttt gagcgtgctt attcgacatc
      841 actataaatt cgagatgatt atttgtttca caatcatcaa gatcgaattc gatcatggac
      901 agagccatta cgagagttct ttctaagtat cctgaattgg gcgtatgacg agatttatcc
      961 ttgatacttt tccgagtcgc aggagctacc ataaaatatt gttcttctga aagaccttct
     1021 aaaagacttg ttttgatcgg ccttggaata acaacattgt ccgcgtctgc actgtaaccg
     1081 attgctactg cagatcttgc taattgcgct ttactaaatc ttgatccact tttatataat
     1141 ttatataaac ttgaattttc atccatatta tctaaagctc tatttgttaa agcttcaaca
     1201 atatagtatc ccaatttaat attattattt ggtaaatttt taaagatctt atttgtttga
     1261 tcgtttttaa gatttaccat gttttctacg tccaaacttg gtgcgtgtga agtagaagat
     1321 ataaagaaga acaattgttt ttctaaagct gctagagaat cataatattt tttattatct
     1381 ttattataat aattatatag ttcttcgcta acactatcta tcaatttttt tgaaagactc
     1441 ttggtaatta taactttatc aaatccacag aatttattaa ataaacatct tccataacta
     1501 tacaattcac cagatctcat ttgaactgta tcatataaat gatcgttcca aagttttata
     1561 gattctggta aatcttttaa agatcttaga tttttaatga ttatattgtc tttattaaat
     1621 ttttcttttg ttagaacaaa tgcagcatat agtgcttcat gtctaattgt cgctaaaaaa
     1681 ctattgtttt gatcataagt aatcgaattt tgtagaaaag ctttatcttc acattccaac
     1741 aaagcttctc tatcatgaat tatatataat gcaaccgtat caccatctaa gtccgcgtta
     1801 agcggttcta agaacgaagg actaacatgt attgtttcat cttctgaatc atctgtcggt
     1861 tcaattagaa ctaccggagc actatgtcta cttagacttg gttggcgatt aataaatcct
     1921 aaccgttttc tctccttatc agataatctt gtttgcattc atatctcctt aatcaaatgc
     1981 atcttctaac atttcttgtt cgattttacg tcttctaatt aaagatttaa ctgatgacat
     2041 ttcaaactca tcatcaatat tcttttcatc actttccggc tggcaaaacc aatttaaaaa
     2101 ttcatcaaat atttctttgt tatcttcaaa atttctttct tgagtatgaa tatcgaatat
     2161 atcataaaat tcataatctt taaattttga aagataatat agaaaatatg gaaaccatag
     2221 tctgtatagt atctttttac tgaccttaat tttgtatgat tctaagctcg ggtcgataac
     2281 aattacagat cgagcactaa attcaatagt ctttccaaga atcgaacttc taatcaatcc
     2341 tgtctttcca gatatacctt caaatattga ttcatagatt ttatcaacgt aatattgaca
     2401 ccttgctgtg gcagcatcat attccggaat tgctacatca acaatttcat caacaatttc
     2461 attatttaag ttatatctta tcttatcaaa ccaatcactt tctttttgcc ggacaatatc
     2521 tttcattggt ttattttgat tgttctgatt gattaaactt atataatgct tattaatttc
     2581 aacatatctt aattcggatc ttttttttgg atctcttacc accggacgaa tatctggtgg
     2641 gagaattttt acaacatcta ttatatattt cttttcaaat aattctttag caatatcaag
     2701 acccaacact tctgcgacaa attttaatga gagtataaaa ctataaagtc cagtaattct
     2761 aataggaact acaaaccacc cttgaacgaa attcatagtg tttactactt taagagtgtc
     2821 agcagtatga gttactgcta gatatcttga tgttgcggca agagcatctg ctttcttcgg
     2881 atctagcaga tgtttatgtt gactaccaac aatcttttta aaatattttc tttttgttga
     2941 ttttataact ggaaacacta atgttatttt tccaaatgtt cttaaacgaa gatcacttgg
     3001 accgcataga accccacacc tatcacacac ttttccacca tctatagatt caatatttaa
     3061 ttttccacag cggcatctaa agtcatgaat tggtccgaaa atagattcac ttagtaatcc
     3121 attctcatct acatttgaat attttccagt ataaaatttt atacttgtta cttcgcctcc
     3181 caaacattcc gtcattttct cctccgactc taatctttgg atatcatatc atttataatg
     3241 tttaatacta ttctctcttc ggttttcttt tttccaagtt tttcagcttc tttggaagca
     3301 tctatattag ctttgctttg gggtttctta tcttttgttt tattttcatg catctctgca
     3361 aacacattta cagcttttac aggtatatta ttaagcatat attgatcatt tttaagctta
     3421 ttgattaacg cattactaat tttaaattta aatcgtataa catcttgccc aaccctattc
     3481 gaacgagttt taaatgatac aaattttcca aaacctctga caggacatgc tccatcattt
     3541 aaagttttat gaagtattaa ttggaataaa tattgagtaa tttgttgaat aggggcgttt
     3601 ggaaactttt tctgaatctc aagttcagca tcctcaggta gtcgaaacac gtctcctcct
     3661 agagttagaa tggaattagt tcacagagtt tctcaccagt ttttaatgta acattatgac
     3721 gaatcggtac cacacatgca aaaccatctt ttacaaatcg aacatcaatt ctgtcagcta
     3781 catcactgtg aattttaata ttaaatacat cagaatcaat gtttgattta tcaattggaa
     3841 tataatacat tcttccttgt aataaattta ttctaccatt tgaagctgtt acaacatttt
     3901 gttcgtttgt tgctaaaaat acataaccaa gttccacaat agcatcacta aatttcgcaa
     3961 gaataaaaat attttttgaa acttcttgag gagaaatttc aaaactttct ggagtttcag
     4021 aaatttgttc ttcagaagaa ttttcttgaa ctgttaaaga atcatcctga agtgttgaaa
     4081 acccttcatc caatttgttt ttcttactca tgttaattaa cctctctttg gttttatttt
     4141 aattacttgt cgagcgagag caacaatatg tttacaaccg ctcggaacat tatattgatt
     4201 tttttctttt ggtctactta taatcgatct aacaaaattt attgatttaa gtaaacttcc
     4261 agctctaaat actgcattgg caaattcata tttaaagctc tcgcaggtac aaaatacttt
     4321 gacaggagaa ttgagatgta ttatttcatc ttgaatttcc atctcttgtt gatatttatc
     4381 agtgctggcg tgtataatta ttttgtcttt tataatatct tgatgaatat tattgaatct
     4441 tacttcgcca atagatttct ctctgtcttt ataagctgct ggaaattttg aaaaataacc
     4501 aagtgttaat gacatttttt aatccctcat cattatatat agaaatttgt gtgatccaac
     4561 ccaacctatt tagattgggt tggatcacaa ttcttttctt ttttagaatt ttttatcagt
     4621 tctaattttg tttaaatttt agatttaatt ttgaattact tatcttctgg aacgtctacc
     4681 cacattttat ttatcctcct tcaaagaata tattaaccga gaccattccc gattaatgtt
     4741 aatataattt ctatataact tctttgatta ttaaaaaatt gtctagtaaa aattagacag
     4801 atttttaaag agaatttaaa tgttattttt ctaaaataat tatttctata tattttatga
     4861 aaatttcgaa aatcgtttag aacagaatat acacttgcct ggttcttcta gatatacatt
     4921 ataattttct ggaagaaacc aggttctgat tttattaaat aatcttactg tcatatcatt
     4981 ccatagtata ttgtatttgc ttttatcatg gtgaaatcca tctggacttt gactacattc
     5041 atgtttcatt ataagttttc tcccattttt gttcataaat tgacaaaagt tttttggatt
     5101 ttgagatttg aacttgaatt gtaagaaatt aatttgatta ttattgattt tgttactcgg
     5161 cgagaactac cacagaccta attgaattgg atacgttaat atcttgaaac aaaatatact
     5221 tacgttttta tatagaaatc ataactataa aagttaaaaa ttctaatttc taaataattt
     5281 ctgtaaagtt tcaactatgt aaagatatct atcaacagat ctgaatctgg agataggatt
     5341 ggaagtaaat ctacattcca atcttatata tttaaacagt tccaaaacaa tctttaagga
     5401 tttttcctat tgaactccaa agcagtttga tagtttttat aacagtggaa ctatggtttt
     5461 tctccgccca gagctttaac attcatcaga taaagaaatt atttactaat ttcttctact
     5521 aataaatcaa gagctttcaa tccagtttca tccaatttaa ttttttcagt tttatatgtt
     5581 ccagtagtta caaaatgttc taaagcttca atagcatctt cttcagatgt gtatgatgcc
     5641 ataatgactg gtactggact atttacaatt agagcaacta aatcagttcc tactacgcta
     5701 aatcctaata taaatttctt tttaataaaa gtagttcctt ttaaattttt tacatattct
     5761 tccttctgaa gatcttttac aaaatcttca acgaattttt tagcagcgct tgccgatttc
     5821 ttaacttcta ataaatgatc ttccattcta taaatcaaat taacttttgc ataaatatta
     5881 aaaagttttt gaactgtctg aataattctg atctctgtaa tttgacttgc gggagcatat
     5941 aacaccgaag tctctccttg tagccacaaa tgtttcgttt tttgatcgat agctagaaga
     6001 ctttttgtag ttttttcatg ttcgcccatt ttctttagat cctttctgtt cacatttacc
     6061 aacctaattt ccgtaaatat ttaccatgcc aaacgtcttg tagaaaacac ca
//
LOCUS       DAOSGS010000046         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11777_length_5949_cov_3.672379, whole genome shotgun sequence.
ACCESSION   DAOSGS010000046 DAOSGS010000000
VERSION     DAOSGS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11777_length_5949_cov_3.672379"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1317)
                     /gene="aepY"
                     /locus_tag="PLQ22_02710"
     CDS             complement(199..1317)
                     /gene="aepY"
                     /locus_tag="PLQ22_02710"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HOH58904.1"
                     /translation="MKIEKFIKMLNSDFYTGVPDSQLKAFCNYLIATYGIDGKHHIIA
                     ANEGNAVGLAAGYHLATGKTPVVYMQNSGEGNIINPVTSLMNDKVYGIPCIFVIGWRG
                     EPGVHDEPQHIYQGEITCKLLEDMDISYMIIDKETTDADVEAKMIEFNKILNTGKQVS
                     FVVKKGTFEFDRKVKYENKNTMLREETIRHIVNVTGEDPIVSTTGKASRELFEIREAN
                     GQNHGYDFLTVGSMGHASSIALEIALQKPEKKIWIIDGDGAVLMHMGSMAVLATNKPK
                     NIVHIIINNSAHETVGGQPTAMANVDIVKVAEGCGYPNAVSVDSFEKLDVELNKAKKR
                     NELTLIEVKCALGARDDLGRPTTTAKQNKEMFMEHLAK"
     gene            complement(1319..2032)
                     /locus_tag="PLQ22_02715"
     CDS             complement(1319..2032)
                     /locus_tag="PLQ22_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH58905.1"
                     /translation="MRNEIAILMAAGLGKRMAPLTEKTPKPLVKVFGKPMIETIIEGL
                     VKREIKHIYVVVGYKKEQFKFLETKYQNLSLIENNEFLTVNNISSIHAVAPIMGKEDC
                     FICEADLYVSDSSIFLADLNHSCYYGKMVKGHSDDWVFEQNSDGRIIRIGKYGDDCFN
                     MCGIAWFKAADAKKIADAINEAYKKPGTYENLFWDDIVNQQIMNIDLIVHEVLDNQIV
                     EIDTVKELAMVDPNYKEYN"
     gene            complement(2044..3348)
                     /gene="aepX"
                     /locus_tag="PLQ22_02720"
     CDS             complement(2044..3348)
                     /gene="aepX"
                     /locus_tag="PLQ22_02720"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOH58906.1"
                     /translation="MEEKKVYACFTTDVIHEGHLNIIKEAKKYGKVIIGALSDEASIR
                     YNKFPTISLEERKKLYRSIPDVSQVIVQNKMHYDDVISLLKPDYVIHGDNWKVGPESV
                     LREHLIKLLDKYGGKLIEVEYTKNENVKKIDAILKEKLAMPEYRRKRLKQLLNICPIV
                     KVIEAHSGLTGLIAEKTVVDTGNGKLDQFDAMWVSSLCDSTAKGKPDIELVDMTSRFR
                     TIDDIMEVTTKPIIFDGDTGGLTEHFVYTVRSLERMGVSAIIIEDKTGLKKNSLLGLE
                     VKQDQDTIENFCNKISAGKKVKLTDEFMIIARIESLILERGMEDALTRAYAYVKAGAD
                     GIMIHSRKEDPTEILEFCDKFRVEDKKTPIVVVPSSFNTITNEELAEHGVNIVIYANQ
                     LTRSAFPVMQQTAMDILKYHRAKEVDDRLMPIKKIVTLIDEL"
     gene            complement(3353..4486)
                     /locus_tag="PLQ22_02725"
     CDS             complement(3353..4486)
                     /locus_tag="PLQ22_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HOH58907.1"
                     /translation="MKIKRNILLNPGPSTTTDTVKMAQVVPDICPREKEFAGLMKGLR
                     EDLVGIVHGKQEKYTAVLFCGSGTLNIDVCLNSLLPEDKQILVVNNGAYSSRAVEVCE
                     HYGMPHINLKFPINEQPDLNIVEKTLKENDNIYIVYTTHHETGTGLLNPIREIGALAH
                     KYGAIFITDTTSSLAMIPIDIEKDNIDFCMASAQKGIMAMTGLSFVIGSRAEIMKSKN
                     YLKRSYYCNLYRQYEYFERTGEMNFTPPVQTIYATIQALKEYYFEGEEAKWARHTRVF
                     NAIHKGLDKLGFKDYIKRELQSGLVVAALYPDDPNWEFIKVHDYCYERGFTIYPGKVE
                     KKGMFRLCALGAIDVKDIEDFFVVFEEALKINNITIPVVYKES"
     gene            complement(4741..5616)
                     /locus_tag="PLQ22_02730"
     CDS             complement(4741..5616)
                     /locus_tag="PLQ22_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOH58908.1"
                     /translation="MNIDKKQFDVLKLLENQKAQRKIAEETKYSLGTVNKILKELSIL
                     NLITDSVMNENGRKILEQYRVRRAVFLAAGFGERLVPITLNTPKPLIRVNGKRIIDGL
                     IDACLAVGIEEIYIVRGYLAEEFDQLLYKYPMVKFLENPLFNETNNISSSMCARHLLS
                     NSYVFEADLIINPKIIQKYHYTSNFLAIKKERTDDWCFEVDKDNYITKEKFGGFNCYQ
                     MVGISYWNKEDGERLKEDIKTVYEMPGGKERYWEQVPLMDFKYKVQVIECNEEDAIEI
                     DTLKELRAIDKSYEM"
BASE COUNT         1826 a         1114 c          721 g         2288 t
ORIGIN      
        1 acaatatcac caacgattgc tcctagcata gtagatttat cctcctatat cataatttac
       61 accttcatta taaaataaaa ttgcaaataa tacaatacag ataaaatagt ttatattcat
      121 atatgaatta actaacttta ttaatatttt gataatcaaa tcttttaatt taaaattttt
      181 aaatgtaaaa tagtattttt attttgcaag atgttccata aacatctctt tattttgttt
      241 tgctgtagtt gtaggacgac ccaagtcatc tcttgcacct agagcacatt taacttcaat
      301 tagagttagt tcatttcgtt tttttgcttt gtttaattca acatcgagtt tttcaaatga
      361 gtcaactgat acagcatttg gatagccaca tccctcagca actttaacaa tatctacatt
      421 agccattgcc gtcggctgac cgcctactgt ttcatgtgca ctattattaa taatgatatg
      481 aactatattt ttaggtttat tagtagcaag aactgccatc gatcccatat gcattaaaac
      541 tgctccatct ccatcaataa tccagatttt cttttctggt ttctgaagag caatttcaag
      601 cgcaatgctt gaagcatgtc ccattgatcc gactgttaag aaatcatatc catgattttg
      661 gccatttgct tctctaattt caaataattc acgagatgct ttacctgttg tagaaacaat
      721 aggatcttca cctgtaacat taacaatatg cctaatggtt tcttctctca acattgtgtt
      781 cttattctca tatttgactt ttctatcaaa ttcgaaagtg ccctttttta caacaaaact
      841 aacttgtttt cctgtattta aaattttatt aaattcaatc atttttgctt caacatcagc
      901 atcagttgtt tctttatcaa taatcatata actaatatcc atatcttcaa gaagtttaca
      961 tgtaatttct ccttgataaa tatgctgtgg ttcatcatga acaccaggct cacctctcca
     1021 accaataaca aagatacacg gaattccata aactttatca ttcattaaag atgtaacagg
     1081 attaataata tttccttcac cactgttttg catgtatact actggagttt ttcctgtagc
     1141 caagtgatat ccagcagcaa gtccgacagc atttccttca ttagctgcta ttatatgatg
     1201 ctttccatct atgccataag ttgcaattaa gtaattacaa aatgctttta attgactatc
     1261 tggaacacct gtatagaaat cactattaag catttttata aatttttcta ttttcatatt
     1321 agttatactc cttatagttt ggatcaacca ttgctagttc ttttactgtg tcaatttcaa
     1381 caatttgatt atccaacact tcatgaacaa ttaaatcaat attcataatt tgttgattta
     1441 caatatcatc ccaaaataaa ttttcatatg tacctggttt tttatacgct tcattaatcg
     1501 catcagcaat ttttttagca tctgctgctt taaaccatgc aattccacac atattgaaac
     1561 aatcatcgcc gtattttcct attctaataa ttctaccatc actattttgt tcaaataccc
     1621 agtcatcgct atgtcctttt accattttcc catagtaaca tgaatgattt aaatccgcta
     1681 aaaaaattga tgaatcagat acatataaat cagcctcaca aataaagcaa tcttctttac
     1741 ccataatagg agcaacggca tgaattgatg aaatattatt gactgttaaa aattcgttat
     1801 tttcaattaa tgacaaattt tgatatttgg tttccagaaa tttaaattgt tcttttttat
     1861 atcctacaac aacataaata tgcttaatct ctctttttac taacccctca ataatggttt
     1921 caatcattgg tttgccaaaa accttgacta atggctttgg tgttttttca gttaatggtg
     1981 ccattctttt tccaagacca gcagccatta agattgctat ttcattcctc atttttgaat
     2041 ctcctataac tcatctatca gtgtgacaat ttttttaata ggcattagtc tatcatctac
     2101 ttcttttgct ctatgatatt ttaaaatgtc catagcagtt tgttgcatta caggaaatgc
     2161 acttcttgtt aactggttgg catagataac aatattaaca ccatgttccg caagttcttc
     2221 attagttatg gtattaaatg aagaaggaac aactacaatt ggagttttct tatcttctac
     2281 tctaaactta tcgcaaaact ctaaaatctc agtagggtct tcttttctag agtgaatcat
     2341 aattccatct gcaccagctt taacatatgc atatgctctg gttagagcat cttccattcc
     2401 tctttcaagt attaaagatt ctattctagc gattatcata aattcatcgg ttagtttgac
     2461 tttttttcct gcagaaattt tattacaaaa attttcaata gtatcctgat cttgttttac
     2521 ttcaagtcct aaaagtgaat tcttttttag tccagttttg tcttctatga taatagcaga
     2581 tacacccatt ctttccaaag atctaacggt atagacaaag tgttccgtaa gtccgccagt
     2641 gtcaccatca aagatgatcg gctttgttgt aacttccatg atatcatcaa tcgttctgaa
     2701 tcttgaagtc atatcgacaa gttctatatc tggtttccct tttgctgtag agtcacataa
     2761 agacgaaacc cacattgcgt caaattgatc aagtttacca ttaccagtat caacaacagt
     2821 tttttcagca atcaaaccag ttagtccaga gtgtgcttca attactttta caattggaca
     2881 tatatttaac aattgtttta gtctttttct tctatattct ggcattgcta atttttcttt
     2941 taaaatggca tcaatctttt taacattttc gttttttgta tactcaacct caatcaattt
     3001 cccaccatat ttatcaagta gtttaatcaa atgttctctt aacaccgatt ctggccctac
     3061 tttccagtta tcaccatgaa taacataatc gggtttaagt aaacttatta catcatcata
     3121 atgcatttta ttctgaacga taacttgact gacatctggt atagagcgat atagtttttt
     3181 tctttcttct agtgaaatag taggaaactt attatatcta atagatgctt catcagataa
     3241 agctcctatg attacttttc catacttttt tgcttcctta ataatattca aatggccctc
     3301 atgaatgaca tcagtagtaa agcatgcata tacctttttt tcttccatat ttttaactct
     3361 ccttatatac tactggtatt gtaatattat ttattttaag cgcttcttca aagacgacaa
     3421 agaaatcttc gatatctttt acatcgattg cacccaaggc gcacaatcta aacatgccct
     3481 tcttttctac ttttcctggg taaatagtaa atcctctttc ataacaataa tcatgaactt
     3541 taatgaattc ccaatttgga tcatcaggat ataaagctgc aaccactaat cctgattgta
     3601 actctctttt tatgtaatct ttaaatccta atttgtctaa ccctttgtga attgcattga
     3661 atactctcgt atgtctagcc cactttgctt cttcaccttc aaaataatat tcttttaatg
     3721 cttgaattgt agcatagata gtttgaactg ggggagtgaa attcatctca ccagttcttt
     3781 caaaatattc atattgacga tataaattgc agtagtaact ccgcttcaaa taatttttgc
     3841 ttttcataat ttcggcacga ctaccaatta caaaagataa tccagtcatt gccatgatac
     3901 ctttctgagc actagccata caaaaatcaa tattgtcctt ttcaatatca ataggaatca
     3961 ttgcaagact tgaagtggta tcggtaataa atatagctcc atatttatga gccaaagcac
     4021 caatttctct aattggattt aataatcctg taccagtttc atgatgagtt gtataaacaa
     4081 tgtaaatatt atcattctct tttaaagttt tttctacaat atttaaatct ggttgttcat
     4141 taatcggaaa cttcaaattg atgtgtggca ttccataatg ctcacaaacc tcgactgctc
     4201 tggatgaata tgcaccatta tttacaacta aaatctgctt atcttcgggt aagagcgagt
     4261 ttaagcaaac atcaatattt aatgttcctg aaccacaaaa taaaaccgct gtgtattttt
     4321 cctgcttgcc atgtactatt cctactaggt cttctctgag acctttcata agtcccgcaa
     4381 attctttttc tcttggacag atatcaggaa ctacttgtgc catcttaacg gtatctgttg
     4441 tagttgatgg ccccggattt aacaaaatat ttctttttat tttcattttt ctaccttctc
     4501 tattttttag ttttttgtta actttttatt tttcatctct tgaaattact caacatttta
     4561 gatatcgttt cttattaatt tagattcgag tattaaataa tattcataat ttcttagata
     4621 agcaagtttt taatataaaa attggtatta attctagact caactctttt gaattcaata
     4681 atctataaag ttgttgagcg tacaaacgat tattttttct ttcctttttt attaaccatc
     4741 ctacatttca taagatttgt caattgctct taattctttt agtgtatcta tttctatagc
     4801 atcttcttca ttacattcta ttacttgtac tttatattta aagtccatca aggggacttg
     4861 ttcccaatat ctttctttac ctccaggcat ttcataaact gtcttgatat cttctttcaa
     4921 tctttcacca tcttctttat tccaataaga aattcccacc atttggtagc aattaaaccc
     4981 accgaatttt tctttggtta tgtagttatc tttatctact tcaaaacacc agtcatctgt
     5041 tctctctttt tttattgcta aaaaatttga tgtgtagtgg tacttttgaa ttatcttcgg
     5101 attgataatt aaatccgctt caaatacata agaatttgat aacagatgtc tagcacacat
     5161 actagatgaa atattgtttg tttcattaaa caaaggattt tctaagaatt ttaccatagg
     5221 atatttataa aggagttgat caaactcttc agctaaatat cctcttacga tatagatttc
     5281 ttcaattcct acagctaaac aagcatcaat aagaccatct ataattcttt ttccatttac
     5341 acgaataaga ggttttggtg tatttaatgt gattggtact agccgttcac caaatccagc
     5401 agcaaggaaa acagctcttc ttactctata ttgctccaat atttttcttc cgttttcatt
     5461 cattacacta tcagtgatta gattaagaat agaaagttcc tttagaatct tatttactgt
     5521 accaagagag tatttagttt cttctgctat ttttctttgt gctttttgat tttctaacaa
     5581 tttcagcaca tcaaattgtt ttttatctat gttcatcatt ttaaccacca tcagcatata
     5641 tgaacaccaa acacttattt gaaaacatta atgttcattt gtggttgtat tatagttcat
     5701 tatgaacaca atgtcaacat tttccaaaat aaaaaagact taattatcta agtccttttc
     5761 aataggtttc aattctttgt aagatattta gttacaattc atcaaataaa ggagtacatt
     5821 tttagatatt ttttcaggga tagaaaaatg tgcttcagca attaaaccgg taatagtagc
     5881 aattgtgttc acatcttcac tgatactaat ggtatcttca aaattatttg cttctaaaaa
     5941 aacagtaat
//
LOCUS       DAOSGS010000047         5931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_11824_length_5931_cov_3.964261, whole genome shotgun sequence.
ACCESSION   DAOSGS010000047 DAOSGS010000000
VERSION     DAOSGS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_5931_cov_3.964261"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLQ22_02735"
     CDS             complement(<1..375)
                     /locus_tag="PLQ22_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH58909.1"
                     /translation="MIMNIKIKETNEEPSLKQMYYAYYAKKARVRLGLTLEQASKLVC
                     SLSYMSKFENMLVTPPAKFLKQIERIYGIEFKKVDWLRIETILNSLLIHFLKGDIEPI
                     KEASDAFDELDFSATGAIVKGYY"
     gene            complement(421..1119)
                     /locus_tag="PLQ22_02740"
     CDS             complement(421..1119)
                     /locus_tag="PLQ22_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOH58910.1"
                     /translation="MKKITEVIIEGNIYSAYFKRSKRSKTALKVTDNLEIIISSPKNL
                     SENEFKKILKNTSLWIESEVIKKKKLYDERKVKEFQDGRVIWLNGKCYDLVPNNKQIA
                     NHLIKGNSIYISKNFSYKKIYNEILEKIEKIFNKYKRLLDFVPNDTILNFKYYKSRWG
                     VCYFKKKEVILNELMVILPDHLIEYIVVHELVHFKYQSHGRYFHDFLEKLISGHMLRQ
                     KELNFFTNILYKKE"
     gene            complement(1116..1814)
                     /gene="radC"
                     /locus_tag="PLQ22_02745"
     CDS             complement(1116..1814)
                     /gene="radC"
                     /locus_tag="PLQ22_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOH58911.1"
                     /translation="MKIKENIMLREIPREERPRERLIKNGPQSLATYELLAIILRTGY
                     KKNSVLDVSKNLLSRFSDLNELSEATIQELKTVPGIGEAKAVELLAAIELGKRINIPK
                     RSQTSLTNPNNVYNFVKEKLQNLNQEYLIALYLNSKSELIQMRTISVGAVNLTVFDIK
                     NILKWGLKYSSTAIILVHNHPSGDPTPSRQDMNATKDIIKAAATVDLSVVDHLIVGKG
                     SYYSFLEKGMMRSL"
     gene            complement(1849..3123)
                     /locus_tag="PLQ22_02750"
     CDS             complement(1849..3123)
                     /locus_tag="PLQ22_02750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOH58912.1"
                     /translation="MFDNVIELINYIENLKKLQKKTNLDHMQSLCDFFSNPEDKLKII
                     HVAGTNGKGSVSSYLENILLENGYNVGKFTSPYVIVFNERIAYNGANISDADLLKYGN
                     YIISKFEEMEKQGIYRPSFFEFMTILAFLYFSKQKNIDYAIIEVGIGGLLDVTNVIKN
                     PVLTITASISLDHQNVLGETIEEIAIQKLGILKEKVPHIALLQEELESIFIEEAKKKN
                     VPLYFVAKGDIKDLKLSLEATEFKFLGRRYKVRLLGEHQAYNASLAILGTKVLEDKKL
                     IFLDYDKVCRGLEKTYWPGRLEIISKNPLMLVDGAHNIGGIESLVNFLKAVKDDKKVI
                     VYFAVSSNKAKDQMIFKLDEVADEIVFSEFHYKRSDKAINLIDYSHIKNKRIASEDEI
                     ELKIINNSDPNSLIIFTGSLYYVSEVIKKLKA"
     gene            complement(3125..5726)
                     /locus_tag="PLQ22_02755"
                     /pseudo
     CDS             complement(3125..5726)
                     /locus_tag="PLQ22_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
     misc_binding    complement(5763..>5931)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1957 a         1093 c          622 g         2259 t
ORIGIN      
        1 ataataaccc tttactattg cccctgtagc tgaaaaatct aattcatcaa aagcatcact
       61 agcttcttta atcggttcaa tatcaccctt taaaaaatga attaataaag aatttaaaat
      121 agtttcaatt cttagccaat ctactttctt aaattcaatt ccataaattc tttcaatttg
      181 ttttaaaaac ttagcaggtg gagttacaag catattttca aatttactca tataactcaa
      241 actacatact aattttgacg cctgctctaa tgttaggcct aaacgcactc gcgctttctt
      301 tgcataataa gcataataca tttgctttaa acttggttct tcgtttgttt ctttgatttt
      361 aatattcata atcataatct tctctccttc aataagtata ttattgaaag aaaataaaaa
      421 ttactctttt ttatataaaa tattagtgaa aaaatttaat tccttctgcc ttaacatatg
      481 acctgaaata agtttttcta aaaaatcatg aaaataacgc ccgtgacttt gatatttaaa
      541 atgaactagc tcatgaacta caatatattc aattaaatga tcaggaagaa tcaccattaa
      601 ttcatttaaa ataacttctt ttttcttaaa ataacaaact ccccatctac ttttataata
      661 tttaaaattt aaaatagtat cattaggaac aaaatctaaa agtcttttat atttattgaa
      721 tattttttct attttttcca aaatttcatt atagattttt ttataagaaa agtttttaga
      781 aatgtaaata ctattacctt taattagatg attagctatt tgtttgttgt ttggaactaa
      841 atcatagcat ttaccattta accaaataac tctaccgtct tgaaactcct ttacttttct
      901 ttcatcataa agcttctttt tcttgataac ctctgattca atccataaac tagtattttt
      961 tagaatcttt ttaaattcat tttcgcttaa atttttagga gaactaatta taatttctaa
     1021 attatcagta actttaagtg cagttttact tcttttactt cgtttaaaat aagcagaata
     1081 aatatttcct tctattataa cttcagttat ctttttcata aactcctcat cattcctttc
     1141 tctaaaaaac tataataact ccccttccca acaatcaaat gatctactac tgataaatca
     1201 actgtagcag cagcttttat aatatcctta gttgcattca tatcttgtct tgatggtgtt
     1261 ggatcaccag atggatgatt atgaactaaa ataatcgccg ttgatgaata ctttagtccc
     1321 cattttaaaa tatttttaat atcaaaaact gttaagttaa cagcccccac cgatatagtt
     1381 cgcatttgaa ttaattcact tttagaattt aaataaagag ctattaaata ctcttggttc
     1441 aaattttgaa gtttttcctt tacaaaatta taaacattgt ttggattagt aagagaagtt
     1501 tgagaccttt ttggaatatt aattctttta ccaagttcaa tagcagctaa aagttctacc
     1561 gcttttgcct caccaatacc cggaactgtt tttaattctt gaatcgttgc ttcactcaat
     1621 tcatttaaat cactaaatct actaagaaga ttcttggaaa catctagaac gctatttttc
     1681 ttataaccgg ttcgtaaaat aatagctaat aattcatatg tagctaaaga ctggggacca
     1741 tttttaatta gtctttctct cggcctttct tctcttggaa tttctctaag catgatattt
     1801 tctttgattt tcatcaccct taattatatt ataaaaatta aaagcaaatt atgcttttaa
     1861 ttttttaatc acttctgata cataatataa acttccagta aatataatca aactatttgg
     1921 atctgaatta ttaattattt ttaattcaat ttcatcttca cttgcaattc gcttattttt
     1981 tatatgagaa tagtcaatta gattaatagc tttatcactt cttttgtaat gaaactcaga
     2041 aaagacaatt tcatctgcta cttcatcaag tttgaaaatc atttgatctt ttgctttatt
     2101 tgaactaaca gcgaaataaa caattacttt tttatcatct ttaactgctt ttagaaagtt
     2161 aactaagctt tctataccac caatattatg agctccatct actaacataa gtgggttttt
     2221 agaaattatt tctagccttc caggccaata tgttttttca agacctctac acactttatc
     2281 ataatctaag aaaattagct ttttgtcttc taagacttta gtacctaaaa ttgctagcga
     2341 tgcattgtat gcttgatgct cacctaaaag tcttacttta taacgcctac caagaaactt
     2401 aaattctgtt gcttctaagc ttaacttcaa atctttaata tcgccttttg ctacaaaata
     2461 taaaggaaca tttttctttt tggcttcttc gataaatata ctttcaagtt cttcttgcaa
     2521 taatgcaata tgaggaactt tttcttttaa aatacctaac ttttgaatag caatttcttc
     2581 aattgtctct cctaaaacat tttggtgatc taaagaaatg cttgcagtga tagtaagaac
     2641 tgggttttta ataacattag tcacatctaa taagccacca attcccactt caataattgc
     2701 ataatcaatg tttttttgct tactaaaata aagaaaagca agaatagtca taaactcaaa
     2761 aaatgatggt ctatatattc cttgtttttc catctcttca aatttactaa taatgtaatt
     2821 accatacttt aataaatcag catccgatat attcgcacca ttataagcaa ttctttcatt
     2881 aaaaactatg acataaggcg atgtaaactt cccaacatta tacccatttt ctaaaagaat
     2941 attttctaaa taactagaaa ctgacccttt accattagtt ccagctacat gaataatctt
     3001 taatttatct tcaggattgg aaaagaaatc gcatagcgat tgcatatgat ctaaattagt
     3061 tttcttttgt aactttttta agttttcaat atagtttatt agttcaatta cattatcaaa
     3121 catcttaact aaaactctta atcaaatcta aaacatcacc taattgttta cgatatgatt
     3181 ctaacttatt cctttcttct tcaactttac tagctggtgc tttagataca aagttaggat
     3241 tttcaagcat cttttcacat ctactaattt cactttctaa ctttgttttt tctaaattaa
     3301 gtttttcttt ttcctcttca aaattaatta aatcttttaa aggaagtgcc attgttaggc
     3361 ccttcaaaac tctaacagct ttatctttta aatcaatttt atctaaaaat tgatagttat
     3421 cataattaca gaatcttttt aaatattcta aattttcctc agttgttttt ttaatgactg
     3481 atctttcgtt tcaaatcaat ttttttagaa ttacctattt tgtattcagc tctaatattt
     3541 ctaacattag taataatatc aaaaattata ttcattttat cccctatatt gaatcttaag
     3601 ttcttgtcta tttcaggcca agaactaatt acaatacttt gggaataata tttttgatag
     3661 atttcttctg taaagaaagg cataaatgga tgtaataatt tcaaaattgt ctttaaacca
     3721 tagtttaaaa cagcacaagt attaattttc ttttctttac ttgtagattt tgtaaagaca
     3781 actttagtca tctctaagta ccaagatgca aaatcatccc aaataaagcg ataaatagct
     3841 ctagctgctt caccaaaatt atacttttca aaatgaggat caacttctct tattacttta
     3901 tgtaaacgag taatgaacca tttgtctagt aaacttaact gttttttatc tatttcttcg
     3961 ccactataag atgcattatc aaattgaatc ttaatataac gcgcaatatt ccaaatctta
     4021 tttatatagt tccaagcagc ttctactttt tcttgagaaa atcttaagtc ttctccaaga
     4081 gaaactgtag tagtaataaa ataccttaaa ctatctactc catatttatc aatcatctct
     4141 tctggtacaa taccattacc taaagatttg ctcatttttc taccaatttc atctctaact
     4201 ataccatgaa ttaaaactcc tttgaatggt ctagtattta agaaatgttt tgattgaaac
     4261 accattctag ctacccaaaa gaaaataata tcatagccag ttactaaaac atcagttggg
     4321 aaataacgtt ttaaatctgg agttatttcc ggccagccca tcgtactaaa cggccaaagt
     4381 gcacttgaaa accatgtatc taaaacatct ggatctcttg tccagccttt ccctttagga
     4441 gcttctgttc caacataaat ctcttccccc ttataccaaa caggaatttg atgtccccac
     4501 cacaactgtc tactaataca ccaatcttga atattttcta gccactgttt aaaagtacgt
     4561 tcgaaacgtt taggatgaaa gtttactttt tcatctgggt tattttgcat ttctaaaaca
     4621 tcagaagcta acttgtccat ctttacaaac cattgttttg ataatctagg ctcaacaata
     4681 accccagttc tttcactatg accaatggca tgtgttatct tttcgatttt tctaaaatat
     4741 ccttcttttt taagatcttc taaaacttgc ttacgacaag caaaacgttc catgccatta
     4801 tatttgcctg ctaaggcatt catcgttcca tcttcattca tacataaagg ttgagctaaa
     4861 ttatgtcttc ttcctacttc aaaatcatta ggatcatgag caggagttac ctttacaaca
     4921 ccagtaccaa atttaatatc tacataatca tcagtgatga ttggaatctt gatattagtt
     4981 aatggaatat aaacttcttt cccaacatat ttcaaatatc ttttatcttt aggattaatc
     5041 attaaagctt ggtcagcaaa cattgtttcc ggtcttgttg tggcaataac taaagagcca
     5101 cttccatcta ctaaacgata ttcataataa tatagacttg cttcttcctc ttcataaata
     5161 acttcgatat ttgataaagc agttttacta gcaatatccc aattgataat tctctcgcct
     5221 tgatagatta aaccttcttg atacattttt gtaaaaacat aattaacagc atgatttaat
     5281 ccttcatcta atgtaaatct ttctcttgta tagtccaaag ataaccctag catcgcccag
     5341 ctatggctaa ttttacctga atataattct ttccatttcc aacattcttc taaaaacttt
     5401 tctcttccta aatcatattt agaaatgcct ttttctctta acattgcttc tacttttgct
     5461 tgagttgcaa tcccagcatg gtccattcca ggaagccata agcaatcgaa tccttgcatt
     5521 cttttacgcc taataataat atcaggaagt gtactatcaa aaacatgtcc taaatgtaac
     5581 tcaccagtta cattaggagg cggtaaaaca attgaatatg gctttttact cgtatcacca
     5641 gctgtaaaat aaccttcctt tacccatttt tcatattttc cctttccaac ttttttaaaa
     5701 tcgtatttta aatctaactc tttcatctca ttcctccata taaaaagtcc ttaaaattta
     5761 attttaagga cgaattcttc gcggtaccac cttaattcat agtatacaac tatgcacctt
     5821 cattagtaat taacgcttac ttaacgaaac tagctactac tatttcacta gttcagctcc
     5881 aagactactt caatagggtt acttgtaatc ttccaccaaa atgattactc t
//
LOCUS       DAOSGS010000048         5747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_12345_length_5747_cov_3.517393, whole genome shotgun sequence.
ACCESSION   DAOSGS010000048 DAOSGS010000000
VERSION     DAOSGS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12345_length_5747_cov_3.517393"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLQ22_02760"
     CDS             complement(<1..935)
                     /locus_tag="PLQ22_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58913.1"
                     /translation="MSNFYNFMALFLMSFIPLMVVAIGAMMSEKGGVTNIALEGIMIL
                     GAFCGVLVVKFVGRAIGINADSSMWTIQSVYLLGLLVAAAAGILVALLHAFASINLAA
                     NQIISATAINTLAPALVVFLTMTLALGQTRGSDEIEVPYALFKIKEMGFLSKIPVLGN
                     LFFKDVNLSIFIGVTILIVSYIILYKTRLGLRIRACGEHPEAADSVGINVYKTRYFGT
                     LASGALAGIGGYMLITNMYTVYNGSVAGYGFLAMAVMIFGNWKPFKIALAAAFFSFLT
                     VLASGIAYIPFLENLGFSNEVLKMLPFIATLVVLI"
     gene            complement(928..2265)
                     /locus_tag="PLQ22_02765"
     CDS             complement(928..2265)
                     /locus_tag="PLQ22_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58914.1"
                     /translation="MNSIVRVIFIVIMSLAILGLFGLSILYIIEKYRSKLNKGKEETD
                     KLELEKVEEPIKVVKEEPKQKEKFDLKEFLNKIRKSPTFKSFKVAVISILIGILLGFL
                     IMVITKPSSSLEGLKRLFIGGLRSKRSIGDVLANAVPIMLTGLAFAFASKTGLFNIGA
                     SGQFIVGAAFSLYAARLLTLPPVIHWFVCVLAGVLAGAIWGAIPGLLKAFFNVSEVIG
                     TIMMNYIGIYLVSIMAVYPPVFDTLYNRVAINHPTAWTPVIGLDKVFGGSKIDIGIFI
                     SILVAVLIFFILKKTTLGYQLKAVGYSSTASKAAGINYKKNIIISMSIAGGLAGLGGV
                     FAYLAKNPLFLSGMSHVPPQGFDGMSVALLANSHPVGIIFTSIFISYLKNSAETLQIV
                     GYNRELASVIIASIIFMAALSKIVSILLDKGIFRRIFKRKAKEEKDLEVNDNV"
     gene            complement(2258..3844)
                     /locus_tag="PLQ22_02770"
     CDS             complement(2258..3844)
                     /locus_tag="PLQ22_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58915.1"
                     /translation="MQENRVLEFKNKSEYAVEMLNITKTFGSLYANNDVTLRVKKGEI
                     HAVLGENGAGKSTLMSVLFGLYQADSGIIKINGEEVEINNPNDANDHNIGMVHQHFKL
                     VDVFTVLENIILGKETRKGLFLDLSECRKKVLELSEKYGLEVDLDAYVKDLTVSQQQK
                     VEILKMLYRDAEILIFDEPTAVLTPQEIESLINTMKNFSEEGKTIILITHKLNEIKAV
                     ADRCTILRRGKYITTIDVTKTSVSKMAELMVGRVVDFSVEKTEAKPKEIVLSINNLSK
                     KLKGQDKLVLNNVSFDIKAGEIVCLAGIDGNGQSELVNVITGLDSDFTGNILFNGEDI
                     SKATIRERIDEGMSHIPEDRQKFGLILNYTLAENMITNQYYKPRFSKRGILKNQEILD
                     YTNNLIEEYDIRSGEGAKTFARSLSGGNQQKAIFARETSKPHNLLIASQPTRGLDVGA
                     IEFVHKTIVTERDKGNAIFVVSFELDEVTDLADRILVIYEGAIVGELDPKKTNHAELG
                     LYMLGTKQNVQKVEAGDHYE"
     gene            complement(3904..5028)
                     /locus_tag="PLQ22_02775"
     CDS             complement(3904..5028)
                     /locus_tag="PLQ22_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH58916.1"
                     /translation="MKKFIAFLVLVLGLGLLVGCSCEDKKEEKTKIVMITDVGTINDK
                     SFNQGTWEGVKAFGGKHADKVEYQYYQPANQATADYVAAIELNIEEGAKVIVTPGFYF
                     SDAIGEMAVKYPDVSFIFIDGVPTNKGAAMDIPANVCSILFKEEEAGFTAGYAAVKDG
                     FTNLGFLGGAPVPAVVKFGIGYIAGAYYAANEMSKEITFPANRYEYLNDFAPNPNHTT
                     KAASWYDAGTEIIFACAGGAGNSVFVAAEGKEGKWVIGVDVDQEAQSAKVISSSMKLL
                     GEAVEQELKALIVDKAFSGGKALSKGVHQDAVGLPTAPNSWRWRTFTTAQYEELLTLM
                     KADGFAAPTTKVELAAFLNDKCGNPAGVAALIDVTQPDSE"
     gene            complement(5127..>5747)
                     /gene="nadE"
                     /locus_tag="PLQ22_02780"
     CDS             complement(5127..>5747)
                     /gene="nadE"
                     /locus_tag="PLQ22_02780"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOH58917.1"
                     /translation="EEVKRQRDLINHQKLDTTYENIQARYRTFTLMNIANKVGGIVIG
                     TSNLSEIALGFTTFNGDNSSMYAINSGIPKTVVRKLLEHYLGIFKEVREEIKGVLKAP
                     ISPELLPGKQNTEEILGSYEINDFILYHKLVNGATDEKLVSLLGYVHKLSKEEALKYV
                     SNFNRRLISSQYKRYNMAEGVKVFDVSLSLEDFKLPGDMNKSNENN"
BASE COUNT         1943 a         1145 c          710 g         1949 t
ORIGIN      
        1 aagattaata ctactaatgt agcaataaaa ggtaacatct ttaatacttc atttgagaag
       61 cctaagtttt ctaagaaagg aatataggca attccacttg caagaacagt taaaaacgag
      121 aaaaatgcag ctgctaaggc aatcttaaat ggcttccagt taccaaaaat cataactgcc
      181 attgctaaga aaccataacc agcaacacta ccattatata ctgtatacat atttgtaatt
      241 aacatataac caccaatacc agctaaagca ccacttgcga gtgtaccaaa gtatcttgtt
      301 ttatatacat taattcccac tgaatcagct gcttctggat gttctccaca agctcttatt
      361 cttaaaccta atcgagtttt atataaaata atataactta caattaaaat cgtaacgcca
      421 atgaatatac ttaagtttac atctttaaag aaaagatttc ctagaacggg aattttcgat
      481 aagaagccca tttctttaat cttaaacaaa gcatatggaa cttcaatctc atcagatcct
      541 cttgtttgac ctaatgctag tgtcatagta aggaatacta ctaaagctgg tgctaaagta
      601 ttaatcgcag tagcactgat aatttgattt gcggcaagat taattgaggc aaaagcatgt
      661 aagagtgcaa ctaaaattcc tgctgccgca gctacaagta aacccaatag ataaacactt
      721 tgaatcgtcc acatcgaact atcagcatta attccaattg ccctaccaac aaacttaact
      781 actaataccc cacaaaaagc accaagaatc ataataccct ctaaggcaat atttgtaact
      841 ccgccttttt cactcatcat tgccccaata gcaacaacca tcaaaggtat aaaactcatt
      901 aaaaataagg ccataaaatt ataaaaatta gacattatca ttcacctcca aatccttttc
      961 ttctttagct tttctcttaa agattcttct aaaaataccc ttatctaaaa gaatagaaac
     1021 aatcttcgat aaagcagcca taaaaataat tgaggcaata attacacttg caagttctct
     1081 attataacct actatttgta aagtctcagc actatttttc aaataactaa taaaaatact
     1141 tgtaaagata attcccactg gatgactatt ggcaagtaag gcaacactca ttccatcaaa
     1201 tccttgaggt ggaacgtggc tcatacccga taagaacaat gggttctttg caagataagc
     1261 aaacacccca cctaaaccag caagaccacc agcaattgac attgaaataa taatattctt
     1321 tttataatta ataccagcag cttttgaagc agttgaagaa taacctactg cttttaactg
     1381 atatcctaaa gtagttttct ttaaaataaa gaaaatcaaa actgcaacta aaattgaaat
     1441 aaaaatacca atatcaattt tagatcctcc gaagacttta tctaaaccaa tgactggtgt
     1501 ccaagcagtt ggatgattaa tcgcaacacg attgtataaa gtatcaaaaa ctggaggata
     1561 aactgccatt attgaaacta aataaatacc gatataattc atcataattg ttccaataac
     1621 ttcactcaca ttaaagaaag ctttcagtag acccggaatt gctccccaaa ttgcccctgc
     1681 taaaactcct gccaacacac aaacaaacca gtgaattact ggtggtaaag taagtaggcg
     1741 cgctgcatat aaagaaaaag cagcacctac aataaattgt ccagaagcac caatattaaa
     1801 caaacctgtt ttacttgcaa aagcaaaagc aagacctgtt aacataattg gaacagcatt
     1861 ggcaagtaca tcaccaatac ttcttttact tctaagccca ccaataaata atcgtttcaa
     1921 accttctaat gaagaagatg gtttagtaat taccataatc aaaaatccta ataaaatacc
     1981 aattaaaatt gaaataactg ctactttaaa tgatttaaat gttggtgatt tacgaatttt
     2041 gtttaaaaat tctttcaaat caaatttttc tttttgcttt ggctcttctt taacaacttt
     2101 tataggttct tcaacttttt ctaattctag tttatcagtt tcctctttgc ctttatttaa
     2161 tttagagcga tatttttcaa taatatatag tatgctaagc ccaaataatc ctaaaatagc
     2221 taaagacata ataacaataa atataactct aacgatacta ttcataatga tcacctgctt
     2281 ctactttttg tacattttgt tttgttccta acatatataa accaagttcg gcgtggttag
     2341 ttttcttagg atcaagttca cctacaatcg caccttcata aataactaaa atacgatcag
     2401 caagatcagt aacttcatca agctcaaaag aaactacaaa gatagcattt cctttatctc
     2461 tttctgtaac tatggtctta tgaacaaact caattgcccc cacatctagt cccctagtag
     2521 gttgactagc aattagtaaa ttatgaggtt tagaagtttc acgtgcaaaa atagcttttt
     2581 gttggttacc cccagataaa cttctagcaa aagtttttgc tccttcacct gatctaatat
     2641 catattcttc aattagatta ttagtataat ccaatatttc ttgatttttt aaaatgcctc
     2701 ttttagaaaa tcttggttta tagtattgat tagtaatcat attctcagct aacgtatagt
     2761 ttaaaattag tccaaacttt tgtctatctt caggaatatg actcataccc tcatcaattc
     2821 tttctctaat agttgcttta gaaatatctt caccattaaa taagatattt cctgtaaaat
     2881 cactatctaa accggtaatc acattaacta attcgctttg accattgcca tcaataccag
     2941 ctaaacaaac aatttcacct gctttaatat caaaactaac gttatttaaa actagtttat
     3001 cttgtccttt taatttttta cttaagttat taattgacag cactatttct tttggtttag
     3061 cttcagtctt ttcaactgaa aaatcaacta ctctacctac catcaactca gccattttac
     3121 taactgaagt tttggtaaca tcaatagtcg taatatattt acctcttctt agaattgtac
     3181 aacgatcagc tacagcttta atttcgttta atttgtgagt aattaaaatt atcgttttac
     3241 cttcttccga aaagtttttc atcgtattaa ttaaagattc aatctcttgt ggtgttaaaa
     3301 cagcagtcgg ttcatcaaaa ataagtatct cagcatcacg atataacatc tttaagattt
     3361 caactttttg ttgttgagaa acagttaaat ccttaacata agcatctaaa tcaacttcca
     3421 aaccatattt ctctgataac tctaaaactt ttttacgaca ttcagacaaa tctaaaaaaa
     3481 gacctttacg cgtttctttt cctaaaatga tattttctaa aaccgtaaat acatcaacta
     3541 atttaaaatg ttggtgaacc atcccgatat tatgatcatt ggcatcatta gggttattga
     3601 tttcaacttc ctcaccattt attttgataa taccactatc agcttggtaa agaccaaata
     3661 aaactgacat taatgttgat ttaccagcgc cattttcacc caaaactgca tgaatttcac
     3721 ctttttttac tcttaaagta acatcgttat tagcatacaa gctaccaaaa gttttagtta
     3781 tgttcaacat ttcaaccgca tattcacttt tatttttaaa ttctaaaact ctattttctt
     3841 gcattaaatt ctcctttttt aaaataagag ggatgtttcc atccctcttt tttatcttcc
     3901 aaattattca gagtctggtt gtgtaacgtc tattaatgct gctactccag ctggatttcc
     3961 gcacttgtca tttaagaaag ctgctaattc aactttagta gttggtgctg caaaaccatc
     4021 tgctttcatt agtgttaata gttcttcata ttgagctgta gtaaatgttc tccatctcca
     4081 agaatttgga gcagttggaa gtccaacagc atcttgatga acacctttac ttagggcttt
     4141 tccaccacta aatgctttat ctacaattag tgctttaagt tcttgttcta ctgcttctcc
     4201 taataatttc attgatgatg aaataacttt tgcgctttga gcttcttggt ctacgtctac
     4261 accaataacc catttacctt ctttaccttc agctgcaacg aacactgagt ttcctgcacc
     4321 acctgcacaa gcaaaaataa tttcagtacc agcatcatac cagctagctg cttttgtagt
     4381 atggtttgga tttggagcaa agtcatttaa atattcataa cggtttgctg ggaaagtaat
     4441 ttccttactc atttcgtttg ctgcataata ggcaccagca atataaccaa ttccaaactt
     4501 aacaacagct ggaactggag ctccacctaa aaatcctaag ttagtgaaac catcttttac
     4561 agctgcataa cctgctgtaa atcctgcttc ctcttcctta aatagaattg aacatacgtt
     4621 tgcaggaata tccatagctg ctcctttgtt agtaggaaca ccatcaataa agatgaatga
     4681 tacatctgga tatttcactg ccatttcacc aatagcatca ctgaaataga atccaggagt
     4741 tacaataacc ttggcccctt cctcaatatt caactcaatt gcagctacat agtcagctgt
     4801 tgcttggttt gcaggttgat aatattgata ttcaacttta tcagcatgct tgcctccaaa
     4861 tgccttaact ccttcccaag ttccttggtt gaaagatttg tcgttaattg ttccaacgtc
     4921 agtgatcata acaatttttg ttttttcttc ttttttgtct tcacaactac aacctactaa
     4981 tagtcctagt cctaaaacta gcacaagaaa tgcaataaat tttttcattt tttcctcctt
     5041 tacgaaaaaa ttgattatta cttacataat ctttacgtcc ttagtatagc aaaaaacgat
     5101 tgtaattgca atcgttttcg ttattttcaa ttattttcat ttgacttatt catatcacct
     5161 ggtaatttaa aatcttccaa acttaaagac acatcaaaga ccttaacacc ttcagccata
     5221 ttataacgtt tatattgact agaaattaat cttctattaa agttagatac atactttaga
     5281 gcttcttctt ttgaaagttt atgaacataa cctaatagtg atactaattt ttcatcagta
     5341 gcaccattaa ctagtttatg atataaaata aaatcattaa tttcataaga tcctaatatc
     5401 tcttcagtat tttgttttcc tggtaaaagt tcaggactaa ttggcgcctt taaaactcct
     5461 ttgatttctt cccttacttc tttaaaaatc cctaagtaat gttctaataa ttttctaaca
     5521 actgttttag gaataccaga gttaattgca tacattgaag agttatcacc attgaatgta
     5581 gtgaacccta aagcaatctc acttaagtta ctagtaccta ttacaatacc gcctacctta
     5641 tttgctatat tcattaaagt aaacgtgcga tatctagctt ggatgttctc ataagttgta
     5701 tcaagttttt gatgattaat taaatctctt tgacgcttta cttcttc
//
LOCUS       DAOSGS010000049         5629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_12717_length_5629_cov_5.123610, whole genome shotgun sequence.
ACCESSION   DAOSGS010000049 DAOSGS010000000
VERSION     DAOSGS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12717_length_5629_cov_5.123610"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLQ22_02785"
     CDS             complement(<1..740)
                     /locus_tag="PLQ22_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58918.1"
                     /translation="MATSSIDTTLSNQVYISRDNIRNQIIEYMQYYLEIENVDLIKSS
                     FLSFLVDTLATLTSNIIFYSSSSYKEFFLTTAQLPESIYNLSAFLGYNPSEASYSTAN
                     VLINIPLGFTDANTSFSIPESFKFYADDIEFLTYYETDITVTNNASVSIVVTQDSTKT
                     YNLPVNIDTTSSDPSFTFILPVRQYKEVEQEFQIDSDIELYQFITIDVPLDGKLSSLE
                     VQVRDPDSASWRLYTEFNSIYLMSATDYG"
     gene            complement(758..1399)
                     /locus_tag="PLQ22_02790"
     CDS             complement(758..1399)
                     /locus_tag="PLQ22_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58919.1"
                     /translation="MQNYNRINDYFVDYWKTLYDFYSKHGQAYLVTYYNLDPEETVWD
                     NENLMGGYYEKIGQYSGVRWKKILTLPVYFIEEIDTIFDAQEIGYVNDQKTGFVIPSS
                     YGITPYPNDIIKLYQNYIREDDRYALYVVSGVQKQTSGDKNYWKLGCVGEQSRTTTEI
                     DLQVSENLIFYDYDKKIHQLSESVSMTKLLSKNETIRENLNGLFDQNSGLYFV"
     gene            complement(1386..1973)
                     /locus_tag="PLQ22_02795"
     CDS             complement(1386..1973)
                     /locus_tag="PLQ22_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58920.1"
                     /translation="MRRLLLDIVINSDTIKKSISNFDRKQLIEAIKILPNNQLIRLCS
                     ILEKKKFDFDKLGRKIERGLLVAAVMPVPATTELYVVYKLITKLNYQCLYKCNVIKND
                     LDKRLCYKKCEVISLEKAITAVKKELGDCWYEKNPEKCRQTIVSYLQDLHERLGKAKI
                     KLSDYQHKVEMSKRDKKLKIAKMKQRELAKKNAKL"
     gene            complement(1984..2766)
                     /locus_tag="PLQ22_02800"
     CDS             complement(1984..2766)
                     /locus_tag="PLQ22_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58921.1"
                     /translation="MNKDSKLIELSCKEVLMNIVKESKSLDKKLSFFQKTLLFDKIKE
                     MKYEKVIGLLFNEGVEVTSEQKREFESKTKKVAKYGTATVAGAYGAKKVSDIITNKKL
                     NALKGLKDKLNTTINFSQNADEIAKAKQKLAQVDGQIKELGKQTLKRSGKFAKFIPKY
                     AKGASGKGVKGAIAAVAGLFLYRKLSDPCVRKNLGNKQAQMACKMEAIKKVVSQIKSD
                     MGKCSGAADPIKCKQKLSKELIKWQTKYQQYLVQMNQSKKNK"
     gene            complement(2759..3307)
                     /locus_tag="PLQ22_02805"
     CDS             complement(2759..3307)
                     /locus_tag="PLQ22_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58922.1"
                     /translation="MDGTIRYCVVKKYSGILIRKHKNNTHLFFIPVDPKKYMYLALIG
                     QLTEEEDTYAAQLLVKLPEEIIDQTIEGEYEYFGERFVEVESLKCKSTNPSPGKYIIT
                     LQKKGFNIKSNLEINYDVEYSTVARRIYLVKGDLNLVTGEVTNQDMKMVAAKQAAFMG
                     NKQRFDEMKRIKPNRRIKYYHE"
     gene            complement(3352..3777)
                     /locus_tag="PLQ22_02810"
     CDS             complement(3352..3777)
                     /locus_tag="PLQ22_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOH58923.1"
                     /translation="MANILDKFKTTSIGSSGRVLDFSSKLSPSGDFNKLFDMDAILTS
                     WSNILMTPRGSMDHDPEFGSDLYLYIFEPADTVTQESIKNEIYKAITTYDDRANIDEI
                     NVAFFKNKKGFNVSIVASYEGDNSEMNLTMDENTYMSFS"
     gene            complement(3781..3930)
                     /locus_tag="PLQ22_02815"
     CDS             complement(3781..3930)
                     /locus_tag="PLQ22_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58924.1"
                     /translation="MKVPYHDIIFMPVKRFYDLIKWKADLEEERQKIMKEQESKFRSS
                     QKQKR"
     gene            complement(3989..4780)
                     /locus_tag="PLQ22_02820"
     CDS             complement(3989..4780)
                     /locus_tag="PLQ22_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58925.1"
                     /translation="MPFTGFNLKYPEYEVITPQTHLSFTTRTLNVQDEERLKGSLMTP
                     TKVTEHLNKCIYETLVKKPDTITEFKDFLKLVTLKDRDTLLYGLYHITYEDIRNYDIK
                     CKNCKKDFSVTVAASDTFNFNPYPGEDILTKRVKVELPKSKGVVAYIKQPTLEDEMNA
                     IKSMSSRPGSTMDIITETLIIERFEQDIEAATEPLVINARLDIIDAYRSLPAKDKREI
                     YEKFNEEFGKYETSLKMQVFCQHCGTEDIVNIDLVENFFRMVYSS"
     gene            complement(4834..5286)
                     /locus_tag="PLQ22_02825"
     CDS             complement(4834..5286)
                     /locus_tag="PLQ22_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58926.1"
                     /translation="MGGVKFAVPGNIDYGNSVSIKFLEFNKTPLLDIFSGWFKLIRDY
                     RTGVTDLIDGNDGFGYTKSQYSALMYYWTTAPDAQTVEFFACYDGMFPTKDPADLYGS
                     DVETVGRLDVEIEFNVDYAWREPWVLQRCQRWAEMFASAKPTVQSYNQ"
BASE COUNT         1769 a          977 c          704 g         2179 t
ORIGIN      
        1 ccataatctg ttgcggacat taaataaata ctgttaaatt cagtgtataa tctccatgat
       61 gcactatctg gatctctaac ttgaacttct aatgaagata attttccatc taaaggaaca
      121 tctattgtaa taaattgata taattcaata tctgaatcta tttgaaattc ttgttctact
      181 tctttatatt gtcttactgg taatataaat gtaaaagaag gatccgatga agtagtatct
      241 atgttgactg gaagattata tgtttttgtc gaatcctgtg taaccacaat agaaacacta
      301 gcgttgttag ttactgttat gtcagtttca taatatgtta aaaattctat atcatcagca
      361 taaaatttaa aactttccgg aatagaaaaa gatgtgtttg catcagtaaa acctaatggt
      421 atgtttatta aaacgtttgc tgttgaatat gatgcttcag aaggattgta tcctaaaaat
      481 gcagataaat tataaattga ttccggaagt tgagcagtag tcaagaagaa ctccttataa
      541 ctggagctag aataaaaaat aatattggac gtaagagtcg caagtgtatc aaccaaaaat
      601 gataaaaaag aactctttat taaatcaaca ttctctatct ctagataata ttgcatgtat
      661 tcaataattt gattacgtat gttatctcta gaaatgtaaa cttgatttga taaagttgtg
      721 tctatactag aagttgccat ctaatgtcct tttaaagtta aacaaaatat aaacctgagt
      781 tttgatcaaa taatccattt aagttttctc tgattgtttc atttttagat aataattttg
      841 tcatggaaac agactcagat aattgatgga tttttttatc ataatcataa aaaattaaat
      901 tttcagaaac ctgcaagtca atctcagttg ttgttcttga ttgttcccca acacatccta
      961 atttccaata atttttatct ccacttgttt gtttttgaac accagataca acatataatg
     1021 catatctatc atcttcccga atataatttt gatataattt gatgatatca tttgggtatg
     1081 gtgttattcc atatgaactt ggaattacaa atccagtttt ttggtcattt acatatccaa
     1141 tttcttgagc gtcaaaaatc gtgtcaatct cttcaataaa ataaacaggt aatgtcaaaa
     1201 tctttttcca tcgaactcca gaatattgtc ctatcttctc ataataacca cccatcaaat
     1261 tttcattatc ccagactgtt tcctccggat ctaaattata ataagtaact aaatatgctt
     1321 gaccatgttt agaataaaaa tcgtataatg ttttccaata gtcaacaaaa taatcattta
     1381 ttctattata gttttgcatt ctttttagct aactcccgtt gtttcatttt tgcaattttt
     1441 aattttttat ctctttttga catttcaact ttatgttgat aatcacttaa ttttatttta
     1501 gcttttccta atctttcatg taaatcttgt aaataggaaa ctattgtttg tctgcatttt
     1561 tcaggattct tttcatacca acaatcacct aattcttttt taactgcagt aatagccttt
     1621 tctaaagata ttacttcaca tttcttataa cataatcttt tatctaaatc atttttaata
     1681 acgttgcatt tgtataaaca ttgataattt aattttgtaa ttaatttgta aacaacatat
     1741 aattccgtag tagccggaac aggcatgaca gcggcaacca ataaacctct ttcaattttt
     1801 cttcctagtt tatcaaaatc aaattttttc ttttctaaaa ttgaacataa tctaattaat
     1861 tgattatttg gaagaatttt gatagcttca attaattgtt tcctatcaaa atttgaaata
     1921 gattttttga ttgtatcact attaattaca atgtctaaaa gtaatcttct caatttacta
     1981 ttcctattta ttctttttag attgattcat ctgaactaaa tattgctgat attttgtctg
     2041 ccatttaatt aattctttag aaagtttctg tttgcattta ataggatctg cagcacctga
     2101 gcattttccc atatcagatt tgatctgaga aactactttc ttaattgctt ccatcttaca
     2161 tgccatctga gcttgtttgt ttcctaaatt ttttctaaca catggatcag aaagttttct
     2221 atatagaaac aatccagcaa ctgcagcaat agcgccctta acacctttac cagaagcacc
     2281 tttggcatat tttggaataa actttgcaaa ttttccactt ctctttaaag tttgttttcc
     2341 taattcttta atctgtccat ctacttgagc caatttttgt ttagcctttg caatttcatc
     2401 agcgttttga gaaaaattta ttgttgtgtt taatttatct ttaagacctt tcagagcatt
     2461 tagtttttta tttgtaatta tatcactaac tttttttgca ccatatgctc cagcaacagt
     2521 tgccgtacca tattttgcaa cttttttagt ttttgattca aattctcttt tttgctctga
     2581 ggtaacttct acaccttcat taaagagaag accaatgact ttctcatatt tcatttcttt
     2641 aattttatca aataataatg tcttctgaaa gaaagaaagt ttcttatcta aactcttact
     2701 ttctttcact atgttcatca aaacttcttt acaagaaagt tcaattaatt tactatcttt
     2761 attcatggta gtattttatc ctcctattag gtttaattct tttcatttca tcaaatcttt
     2821 gtttgtttcc cataaaagcc gcttgttttg ctgcgaccat tttcatatct tgatttgtaa
     2881 cttctccagt tactaaattt aaatcacctt ttactaaata aattcttctc gctactgtac
     2941 tatattcaac atcataattt atttctaaat tagatttaat attgaatcct tttttctgaa
     3001 gagtgatgat atatttacca ggagatggat tagttgattt acattttaaa gattcaactt
     3061 caacaaatct ttctccaaaa tactcatact ctccttcaat agtttgatca ataatttcct
     3121 ctggaagttt aactaataat tgagccgcat atgtatcttc ttcttcagtt aattgaccaa
     3181 tcaaagctag atacatatat ttttttggat caacaggaat aaaaaataag tgtgtattat
     3241 tcttatgttt ccttattagt attccactat attttttaac tacacaatac ctaatagttc
     3301 catccataat ttaataagtc tccgatggta tatttttaat ttgttctaat tttaagagaa
     3361 actcatgtat gtattttcat ccattgttaa attcatctca ctgttatccc cctcataact
     3421 agcaactatt gaaacattaa aacctttttt atttttaaaa aaagctacat ttatttcatc
     3481 gatatttgct ctatcgtcgt atgtagtaat tgctttataa atttcattct taatactctc
     3541 ttgagttaca gtatccgctg gttcaaaaat atacaaatat aaatcacttc caaattccgg
     3601 atcatgatcc attgatcctc taggtgtcat taatatatta ctccaagatg ttaaaattgc
     3661 atccatatca aataatttat taaaatctcc acttggagaa agcttagaag aaaaatccaa
     3721 aactcttccg gatgaaccaa tagaagtagt tttgaattta tctagaatat ttgccatgat
     3781 ttaccttttt tgtttttgag aagatctaaa tttagattcc tgttctttca taattttttg
     3841 tctttcttct tctaaatctg ctttccattt aattaaatca taaaagcgct taacaggcat
     3901 aaaaataata tcatgatatg gaaccttcat aagctccata agtgaaaaga tattctcagt
     3961 taaatttttc ctgtacaacg agatttcgtt atgaagagta caccatgcga aaaaagtttt
     4021 ccactaaatc aatatttaca atatcttctg ttccacaatg ctgacaaaat acctgcatct
     4081 ttaatgaagt ttcatatttt ccaaattcct cattaaattt ttcataaatt tctcttttgt
     4141 ccttagcagg taaagatctg tatgcatcaa ttatatccaa tcttgcatta ataactaaag
     4201 gttcagtagc cgcctcaatg tcctgttcaa atctttcaat aattaatgtt tcagtaatta
     4261 tgtccattgt agaaccaggt cttgatgaca tactcttaat tgcattcatt tcatcttcca
     4321 atgtaggctg tttaatgtat gcaactactc cctttgattt cggaagttcc accttaaccc
     4381 tttttgttaa tatgtcttca cctggataag gattgaaatt aaatgtgtca gatgcagcaa
     4441 cagttacaga aaaatccttt ttacaattct tacatttaat atcgtaattt ctgatatcct
     4501 catatgttat atgatataaa ccatataata atgtatctcg atcctttaaa gtaactaatt
     4561 ttaaaaagtc tttgaattct gtaatagtgt ctggtttctt aaccaatgtc tcgtaaatac
     4621 atttgtttaa gtgctctgtt acctttgttg gagtcattaa agaccctttt aatctctcct
     4681 cgtcctgaac atttaaagtc cttgttgtaa atgataaatg tgtttgtgga gtgattacct
     4741 cgtattccgg atactttaag ttgaaacctg tgaatggcat tttttaaact cctttctttt
     4801 cgctttctat gttttagtta ttcttgttat atcttattga ttgtaactct gtacagttgg
     4861 ttttgctgat gcaaacattt ccgcccaacg ctgacatctc tgaagaaccc aaggttctct
     4921 ccaagcatag tcaacgttga attcaatttc cacatccaat ctcccaactg tttcaacatc
     4981 gctaccatac aaatcagcgg gatccttagt tggaaacata ccgtcataac atgcaaagaa
     5041 ttcgacagtc tgtgcatctg gagcagttgt ccagtaatac attaatgcag agtattgaga
     5101 tttagtatac ccaaatccat catttccgtc aattaaatct gtaacaccag ttctgtaatc
     5161 ccgaattaat ttaaaccaac ctgagaaaat gtcaagaagt ggtgtcttgt tgaattctaa
     5221 gaatttgata gatactgagt taccgtaatc tatattgcca ggaactgcga attttacccc
     5281 tcccaatcca gtaaattcaa ccttgtttaa agttccgccc ggaggagtta cacttaaaca
     5341 agaagccgct aaaacgtcct gaatttctga tgtatttgaa agtccagagt taaaagtttt
     5401 tgtataatcc gtaagcttgg gtggtaaata atcaaaccat ataaagtgat cgaaataata
     5461 ttcaaagaaa ttcgttagat ttcttccgct ttattcaagc agcttaatgt ttccattaag
     5521 tctagactat atcatcatct tctatttttt taatagttaa gatggtcccc atttcggagt
     5581 cacttgaccc ctactctctt tcgagatagt cgttgcacgt tctttgaga
//
LOCUS       DAOSGS010000050         5595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_12827_length_5595_cov_8.235199, whole genome shotgun sequence.
ACCESSION   DAOSGS010000050 DAOSGS010000000
VERSION     DAOSGS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_5595_cov_8.235199"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..790)
                     /locus_tag="PLQ22_02830"
     CDS             complement(23..790)
                     /locus_tag="PLQ22_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOH58927.1"
                     /translation="MDILNQFSKIVIAQVGCGGTGSWLVPFVSKLANNIKLRTVSDDF
                     INYTIFDDDIVEQRNILRQNFSSWDIGKNKALALVSKNVFEFQDIVAGIKRVKKYVDI
                     LPDNLPAVDQLPTLFIILGCCDNNKARQDLFKISKLISKKIKSSVVYIDSGNLLYNGQ
                     IITKTFNFPEDNQQDLFKKKKKRKDKINFKKMFPIESDNSNIPQQSCAFFGDQSQAIN
                     IMAATLMFINLQKVLISLELPPEVITFNSSGYSTFEL"
     gene            complement(765..>1250)
                     /locus_tag="PLQ22_02835"
     CDS             complement(765..>1250)
                     /locus_tag="PLQ22_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58928.1"
                     /translation="LHDQIISGAKAAYEYDENFEMSNRYIRYLQIHSHNTMGANFSGI
                     DDTDENYSSLCFCGVIGKITLDSKFYNVDMKYRIWGGTRFIDLGFDDVFDVGYRSKTL
                     TDEQIQKLNTIIEKSKEASKIPKQSSFNFNGLTTQNDDDFFKHLAEESNLLYGYSQPV
                     F"
     assembly_gap    1251..1350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1401..2423)
                     /locus_tag="PLQ22_02840"
     CDS             complement(1401..2423)
                     /locus_tag="PLQ22_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58929.1"
                     /translation="MSLRTRSEIIPLTLNVYKPVETKNSPFDTIVYIFNNNEKFLVEN
                     PQIGKTIVADIHSIQKLFYLTEVKTDWLRTFDGQYVKFENETVFHFLPLFYESVRYPT
                     LTFEIFNRNAPTFRTINKDVNVNGNRHLVRCIDNEIIESSSKTKETTYNYLFQNVKPF
                     QTDNFQLELDEDFQHHLYYEDGVTLLPNIKITCTLPVFQLYAIMHRSASYGYNCELLA
                     MKIDNTFYRFPYGNIYQSDQMCFGINSNRVKIEPPSIAEACYSHIITSNFNGDFTPMI
                     NFDNTVQISLDIEYVREKISRDDFSISFIDVLLYLSSCKDPSDVNLNLFLQSPNVPDL
                     NKKEES"
     gene            complement(2380..2751)
                     /locus_tag="PLQ22_02845"
     CDS             complement(2380..2751)
                     /locus_tag="PLQ22_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58930.1"
                     /translation="MSQKLLLNTKMFNDLLYTNEMKEQLSIIPSKNRYYRPIWTFRYG
                     RDMLSNENNIKSLKRLEKKLTKSIIAWILTSNKSRTCNVRDTKRTIRIVGNKLYIKKD
                     IEQGELDYEFEDEIRNNSTNT"
     gene            complement(2764..3141)
                     /locus_tag="PLQ22_02850"
     CDS             complement(2764..3141)
                     /locus_tag="PLQ22_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58931.1"
                     /translation="MIVGETFMVNIHNLSIDECINLLVLKQDPELIERLKDLIIIKKL
                     IKVISERDQITGYRLLYNMIYRIDKYCRKKFFTIGDNEYKDEELWRQFNEFQRIHSAK
                     CYSRKEILFTIFYAERAIVELYY"
     gene            complement(3217..3579)
                     /locus_tag="PLQ22_02855"
     CDS             complement(3217..3579)
                     /locus_tag="PLQ22_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58932.1"
                     /translation="MAELKYCPHCDTSRECTKKGKNAAGKQLYYCKTCKKTFLPTTAT
                     SASSKVKKTKVKKSLTETDIIVNNNVIKTVPGFISLDQAFDLAKSYFREIAKEKAEVQ
                     ENNSKRTIKFKVTAGTKG"
     gene            complement(3660..3860)
                     /locus_tag="PLQ22_02860"
     CDS             complement(3660..3860)
                     /locus_tag="PLQ22_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58933.1"
                     /translation="MSNEVTIEFTLDELDALNLILTKLQETETIEPKEIDNETNAFIE
                     NLLIKIAKAKFQQDIIKEIPKI"
     gene            complement(3874..4740)
                     /locus_tag="PLQ22_02865"
     CDS             complement(3874..4740)
                     /locus_tag="PLQ22_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58934.1"
                     /translation="MNFFQDLLKIYSAIKFPEQYLEISKVDKAISWYYGDAANQYITL
                     TKTENNNIIEIDIRQAFTAICKCLFEPDSDFIIEMNKIQDKKSRNIFIATSLVNSEYL
                     RVLNIICKVIIMGVLFESSEIILLELKKDGATISCDDETLVRLMNIHEHPLGDFSKMV
                     IDNNFQFHFTQYDKYIRSNRTSYFWDGNTLDVKGTYKHVPPKLNEVQKKILEEEFNDF
                     QEILKIYSKQYFTILTTNNLMKLLKEYYLCGNERYLADGGKYVTKLNNSDISPRNYLK
                     TFIYPIVLSTKI"
     gene            complement(4805..5377)
                     /locus_tag="PLQ22_02870"
     CDS             complement(4805..5377)
                     /locus_tag="PLQ22_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58935.1"
                     /translation="MKIIREGHATPEILTKIDCKTNIIMCIHPAERDTIEETKNFPDR
                     FHIKLARDINLSTNDIVSISSSALATTIRPKLKISFPVGIVPVFDNFNTDNCQDIFWK
                     VYKSSDSYSSYKEGDILNHHIIDNIKFILYMDTIRMRESGLQMILRKDTPILDFFFSY
                     YIQTKTEMAQIEDIYLIIDEQVYNDFVNIF"
     gene            complement(5374..>5595)
                     /locus_tag="PLQ22_02875"
     CDS             complement(5374..>5595)
                     /locus_tag="PLQ22_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH58936.1"
                     /translation="CWGKTAENAVENVKSGALVLAEGSISYRTYVNKEGIKNNHIAEV
                     KISQLTKWANALDADNTKKEKYSESEDNS"
BASE COUNT         1727 a          865 c          716 g         2187 t
ORIGIN      
        1 tcagatcttg ccgataaatt ttttacaact cgaaagttga ataaccactc gaattaaatg
       61 taattacctc aggtggtaat tctaaagata tcaatacttt ctgcaaattt ataaacatta
      121 aagttgcagc cattatattg atagcttgtg attgatctcc aaagaaagca caactctgtt
      181 gtggaatatt actattatca ctttcaattg gaaacatctt tttaaaattg attttatctt
      241 ttcttttttt cttcttttta aataaatctt gttgattatc ttctggaaaa ttaaaagtct
      301 tagttataat ttgtccgtta tatagtaggt ttccactatc tatataaact actgagcttt
      361 taattttttt tgaaattagt tttgatattt taaatagatc ctgtcttgcc ttattgttat
      421 cacaacatcc aagaatgata aataacgttg gcaattgatc tactgcagga agattatctg
      481 gtagaatatc aacatatttt tttactcttt taataccagc tacaatatct tgaaattcaa
      541 atacattttt actaaccaaa gcaagagctt tatttttacc tatatcccag ctactaaaat
      601 tttgacgcag aatatttctt tgttcgacaa tatcatcatc aaaaatagta taattgataa
      661 aatcatcact aactgttctt aatttaatat tgttagctaa tttagatacg aatggaacaa
      721 gccatgagcc tgttccaccg caccctactt gtgctataac gattttagaa aactggttga
      781 gaatatccat atagtagatt actttcttca gctagatgtt taaagaaatc atcatcattt
      841 tgagttgtta aaccattgaa attaaacgat gattgttttg ggattttcga tgcttctttt
      901 gatttctcaa taatagtatt taatttctga atttgctcat ctgttaatgt ctttgaacga
      961 tatccgacat cgaaaacatc atcaaaacca agatcaataa atcttgttcc gccccaaatt
     1021 cgatatttca tatcgacgtt ataaaattta ctatcaagag tgatcttgcc gataacacca
     1081 cagaaacaaa gagaggaata attttcatct gtatcatcaa ttcctgaaaa atttgctccc
     1141 atagtgttat gactatgtat ctggagatat ctaatatatc tgttcgacat ttcaaaattt
     1201 tcatcatatt cgtaagcagc tttagctcca gaaattattt gatcatgcaa nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctaaacttg tagtattgat tttttgtaat
     1381 tgattattga tttttagcat ctaactctcc tcttttttat taagatccgg aacatttgga
     1441 gattgaagaa ataagttaag attaacgtca cttgggtctt tacatgacga aagatatagt
     1501 aaaacatcta taaatgaaat tgaaaaatca tctcttgaaa tcttttctct gacatactca
     1561 atgtctaatg atatttgaac tgtattatca aaattaatca ttggagtaaa atctccattg
     1621 aaatttgaag taattatatg agaataacac gcttctgcaa ttgagggtgg ttctattttt
     1681 acccgatttg aattaattcc aaaacacatc tgatcacttt gatatatatt tccataagga
     1741 aatctgtaaa atgtattatc aattttcatt gcgagtagtt cacaattata tccatatgat
     1801 gcgcttcgat gcattatagc atataattga aaaacaggaa gggtgcatgt tatttttata
     1861 ttcggcaaga gcgttacgcc atcttcataa tataaatgat gttgaaagtc ctcgtcaagc
     1921 tctaattgaa aattatctgt ttgaaatggt ttcacatttt gaaataagta attgtaagtt
     1981 gtttcttttg tcttacttga actctcaata atttcattat ctatgcatct aactagatgt
     2041 ctatttccat ttacgtttac gtctttatta atggttctaa atgtaggtgc attccgatta
     2101 aaaatttcaa acgttaatgt cggatatcta actgattcgt agaagagtgg caaaaaatgg
     2161 aacacagtct cattttcgaa tttaacatac tgcccatcga atgttcttaa ccaatctgtt
     2221 tttacttcag ttagataaaa taatttttga attgaatgaa tatcagcaac aatagttttt
     2281 ccaatttgag gattttcaac aagaaatttt tcgttattgt taaaaatgta tactatagta
     2341 tcaaatgggg aattttttgt ctcaactggt ttatatacat taagtgttag tggaattatt
     2401 tctgatctcg tcctcaaact cataatctaa ttctccttgc tcaatatctt tttttatata
     2461 tagtttattt ccaacgattc ttattgttct ctttgtatct cggacattgc atgttctact
     2521 tttattgctg gttagaatcc acgcaatgat actttttgta agtttttttt ctaatctttt
     2581 taaactttta atattattct catttgataa catgtctctt ccatagcgaa atgtccatat
     2641 tggacgataa tatcgatttt ttgagggaat tattgataat tgttctttca tttcattcgt
     2701 atataataaa tcattaaaca ttttagtatt caatagtaac ttttgactca tgtgtcctcg
     2761 ctttcaatag taaagttcta ctatagctct ttccgcataa aaaatcgtga acaatatttc
     2821 ttttctacta taacattttg ctgaatgaat tcgttgaaac tcgttgaatt gtctccataa
     2881 ctcttcatct ttatattcat tatctcctat ggtaaagaat ttttttcggc aatacttgtc
     2941 tattctatag atcatgttat aaagtagacg gtatcctgta atttgatcgc gctcagaaat
     3001 cacttttatt aattttttaa tgataattag atctttaagt ctttctatta attcaggatc
     3061 ttgttttaat actaagagat taatacattc atctattgat aaattgtgaa tatttaccat
     3121 aaatgtttcg ccaactatca ttatccactc ctttcttttc acataattaa aaaatctggt
     3181 cagaacttaa acagttctga ccagatgtaa aattcactag cctttagtac cggctgttac
     3241 cttaaattta atggttcgtt tactattatt ttcttgaact tcagctttct ctttagcaat
     3301 ctctctgaag taggattttg ctaagtcgaa tgcctgatcc aaggaaataa atccaggaac
     3361 agtcttgata acattgttgt taacaataat atcagtttcc gtcaaggatt tcttcacttt
     3421 cgtcttcttc acttttgaag aagcagaagt tgcagtcgta ggtaagaatg ttttcttaca
     3481 ggtcttgcaa taatataact gtttccctgc agcatttttt ccctttttcg tacattctct
     3541 gctggtgtca caatgtggac aatactttaa ctctgccatt tttacgcctc ctttaagtgt
     3601 ttttaaataa aaatattaaa ctgtctatta taattatttc tatataaaat tctcaaatat
     3661 cagatttttg gtatttcttt tataatatct tgttgaaatt ttgctttagc aattttaata
     3721 agaagatttt cgataaatgc atttgtttca ttgtcaattt cttttggttc aattgtttct
     3781 gtttcttgta attttgttaa tattagattg agggcgtcta attcatctaa tgtaaactct
     3841 atagttacct catttgacat aattttctcc tttttaaatt ttcgttgata atacaattgg
     3901 atatataaat gtctttagat aatttcttgg actgatatct gaattgttta attttgtgac
     3961 atatttgccg ccatcggcaa gatatctttc attgccacat aaataatatt cttttagtaa
     4021 tttcattaga ttgtttgtag tcaaaattgt aaaatattgt tttgagtaaa tctttaatat
     4081 ctcttgaaag tcattaaact cttcttctaa aatctttttt tgaacctcgt taagtttagg
     4141 aggaacatgt ttataagttc ctttaacatc taacgtatta ccatcccaga aataactagt
     4201 tctattactt ctaatatatt tgtcatattg ggtgaaatga aattgaaagt tattatctat
     4261 aaccatttta ctaaaatctc caagaggatg ctcatgaata ttcattaatc ttactaatgt
     4321 ttcatcatcg caagatattg tagcaccatc ttttttcaac tctagtaaaa taatttcgga
     4381 agattcaaat aaaactccca ttataataac tttacatata atatttagaa ctctcaaata
     4441 ttcactatta actaaacttg ttgcaataaa tatatttctt gatttcttgt cttgtatttt
     4501 attcatttct ataatgaaat cactatcagg ttcaaagaga catttacata ttgcagtgaa
     4561 tgcttgtctg atatcaatct caattatatt attgttttct gtttttgtta aagtgatata
     4621 ttgatttgct gcatctccat aataccaact aattgcttta tcgactttcg atatttccaa
     4681 atattgctct ggaaatttaa tagccgaata tatttttagt agatcttgaa aaaagttcat
     4741 atgttaatac cattgttgtt aaaattcggc aagatctagt ttcagatctt gccgaattta
     4801 aaacttaaaa tatatttaca aaatcattat atacctgttc atcaataatt aaatatatat
     4861 cttcaatctg tgccatttca gttttagttt gaatgtaata cgagaagaag aaatctaata
     4921 ttggagtatc ttttcttaag atcatttgta aaccagattc tctcattcta attgtatcca
     4981 tatagagaat aaatttaata ttatctataa tgtgatgatt taaaatatca ccttctttat
     5041 aggaactgta agagtcagaa gatttataaa ctttccagaa aatatcttga caattatcgg
     5101 tattgaaatt gtcaaacaca ggtacaatac caactgggaa actgattttt aattttggtc
     5161 taattgtagt ggcaagagca cttgatgaaa tactaactat atcattcgtt gatagattta
     5221 tatctcttgc caatttaata tgaaatctgt ctgggaaatt ttttgtttct tcaatagtgt
     5281 ctctctcagc cgggtgaatg cacattatga tatttgtttt acaatcaatt tttgttaata
     5341 tctccggtgt cgcatgtcct tctctaataa ttttcatgaa ttgtcctcac tttcagaata
     5401 tttttctttc tttgtattat ctgcatctag agcatttgcc cattttgtta attgagagat
     5461 ctttacttct gcaatatgat tatttttaat cccttcctta ttaacatatg ttcgatatga
     5521 aattgatccc tccgccagaa ccaaagctcc tgatttaaca ttttctacag cattttctgc
     5581 tgtctttccc caaca
//
LOCUS       DAOSGS010000051         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_13417_length_5405_cov_5.421495, whole genome shotgun sequence.
ACCESSION   DAOSGS010000051 DAOSGS010000000
VERSION     DAOSGS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13417_length_5405_cov_5.421495"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1253
                     /gene="dcm"
                     /locus_tag="PLQ22_02880"
     CDS             111..1253
                     /gene="dcm"
                     /locus_tag="PLQ22_02880"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOH58937.1"
                     /translation="MSKKLRVFEAFAGIGAQNTALRRAKIDYEIVGISEWYINALNVY
                     DAINCGKECVKIPSYDDQIRYLKQFTFSRDSVTSIKDITKLSRNEIENLYIVNHRVKN
                     FGSITEISGKNMPDCDLLVYSFPCQDLSTGGKTLGMAKGSGTRSCLLWEIERILRELH
                     VQNRLPEYLLMENVKTIFAQSNKKDLDEWLKFLNSIGYKNGSHMVLNSLNFGVPQDRK
                     RAFIISRLNNEIDDKEILNHKEDRPSTLDFLRFDYNSDDLLRIEADKAQLNNTISREQ
                     MWKINKREPVNSNTIINTITCNMDRSNTAAMFKYDGPKGNTYRLLTIREAFLLMGFTE
                     EEYRRTLKCDLSYRKMNKLIGNSIVVNVLSKIFNYLFKEKYGEVNG"
     gene            1246..2256
                     /locus_tag="PLQ22_02885"
     CDS             1246..2256
                     /locus_tag="PLQ22_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58938.1"
                     /translation="MGKNIYLPIDTKSLYYNIHNDNKTGHTCTVATPIKQGKSVNSEL
                     AKIYDIPKSIDLDYDVELVDYLNKSSKSTTRIDKAFIFNKYTVNGIHINCDADYCIFI
                     KEEIDSNKKQFGRLKLHYPISAFYDGKYSFDNKKVINAISNKLNNFAFIVRGFEYSFD
                     TDILNFDVDVIGYNNIPYSKVFINNKGVGNKHIANYVDIYENYDYEVMGLREKFNKEI
                     INTKNYFELYHIAKIEALNIVAEYLNVKKDFLSISYPYSFYDLSTYKNNVKKYIIVFF
                     TTAKELYFNISMKQLSFFNMFKNYIEIYNVTNLFNNYKINRFSYEDLTKFHKTISTIV
                     LR"
     gene            2268..3728
                     /locus_tag="PLQ22_02890"
     CDS             2268..3728
                     /locus_tag="PLQ22_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH58939.1"
                     /translation="MAKTIQLYPPAKKLINSMRCMGYSFSTSLADIIDNSISANADRV
                     DVIYDTLCDFPYVQIIDNGIGMSYDELVNALVFGTERDYSENEFGRYGLGLKVASLAQ
                     CRKLTVCSKKQGKINCLGYNLDVIEKTNNWDVLVYSYSEIKSIPNIEKLNEVSSGTIV
                     MWQDFDFLRAESSEKSFKDNLRKNVVDARNHVALVFHRIEQTKIYFNENLIPKRDPFL
                     LDSKPRQITGIVSTIDVFDSKIMVTPHTLPNANTLTSEERELLGIKNGVSIYDGQGFY
                     IYRNKRLIIWGSWLRMNSRSEFNKLARIQIDLPSSLDSIWELDVKKSTAKIPEIIKEK
                     LKATIVDSMGKSRRVIVMPGKVEEAAPNKIWLRREFGKEQVNYELNRESSLFKKIYDE
                     IGSSLLPYFEAYLKQIEKNLPKFKIKDDIEENFKFLNNVAETRDDLKKELIEILRDVN
                     SNNKEELLKYLINQKRYEILKDDLESIMKEINNGGI"
     gene            3715..5382
                     /locus_tag="PLQ22_02895"
     CDS             3715..5382
                     /locus_tag="PLQ22_02895"
                     /inference="COORDINATES: protein motif:HMM:NF022063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HOH58940.1"
                     /translation="MEEFKNLLYNMLRVVFKGKDAQHSELIKQAKIISKNIEKNILHI
                     SLSPEDKEAAIDMVINQYEMNIGVVQPNPSFLSDGEPGEWFLNEKDNNSFEFFSRYCE
                     YLRDDAQFSDSTIDKIKLSTESILSYCANPKGRTSKENQKKRGLVVGDVQSGKTANYI
                     ALINMACDVGYNIVVLLAGMTSILRQQTQDRIDSGFIGAKSDTISNIPEFVGVGVELK
                     RYFGIPLTNHKNDFVKYVKENQNAGAQDFNKPIVLVVKKNKSILNSVSQWLKPGLNNI
                     QTSNILIIDDEADNASVNTRKDINEISAINECIRKIYNNFPIASYVGYTATPFANIFI
                     NPFESSLENQDLFPANFIELLETPSNYFGFDKVFEKDSNDNYLHIQLINESDVYNIPA
                     LHKRDYIVKDLPDDLIESIYHFYLVNCIRTLRGDTKKHRSMLINVTRFNDVQYELFDL
                     VERYHNNFVNLLEEINSKISRKPGICSVSQKLFELYLKDKTFSKARREFSWKDICRVL
                     LHEAKQIVIRVINNKTKKTDRFNYDDYKDDGARAIIIGGFVLSRGLT"
BASE COUNT         2034 a          639 c          955 g         1777 t
ORIGIN      
        1 agtttcgatt agtagatggg aacgtggtga acatgaacca actaaactag ttaaagtacg
       61 attaaaagaa ttagctaaag aagacgaaat agatttatag gtgaatgata atgagtaaaa
      121 aattaagagt gtttgaggca tttgcgggca taggtgcgca aaatactgca ttacggagag
      181 caaaaattga ttatgagatc gttggaatta gcgaatggta cattaatgca ttaaatgttt
      241 atgatgctat taattgtgga aaagaatgtg taaaaatacc tagttatgat gatcaaattc
      301 gatatttgaa acaatttact tttagtcgtg attctgtaac ctcgataaaa gatattacta
      361 aattatctcg aaatgaaata gaaaatttgt atattgtgaa tcatagagtg aaaaattttg
      421 gatcaattac tgaaatatct ggcaaaaata tgcctgattg cgatttgctt gtttattctt
      481 ttccttgtca agacttatca acaggtggaa aaacattagg aatggcaaaa ggatctggta
      541 cacgctcgtg cttgttgtgg gagatagaaa ggatattaag agaattacat gttcaaaatc
      601 gtcttccaga atatttgctt atggaaaatg ttaaaacaat atttgcgcaa tctaataaga
      661 aagatttgga tgaatggcta aagttcctta attcgattgg ctacaaaaat ggaagtcata
      721 tggttcttaa ttccttgaat tttggagttc ctcaagatag aaaaagagca tttattataa
      781 gtcgtttaaa taatgaaatt gacgacaaag aaattttaaa tcataaagaa gatagaccat
      841 ccactttgga ttttctaaga tttgattata attctgatga tttgctgagg atcgaggcag
      901 acaaggctca attaaataac acaatttctc gtgaacaaat gtggaaaatt aataaaagag
      961 agccggttaa ttctaataca atcattaata caattacttg taatatggat agaagtaaca
     1021 ctgccgctat gtttaaatat gatggaccaa aggggaatac ctatagactt ttaactatta
     1081 gagaggcttt tcttttaatg ggatttaccg aagaagaata cagacgcaca ttaaaatgtg
     1141 atttgagtta tcgaaaaatg aataagttga ttggtaactc tattgttgta aatgttcttt
     1201 ctaaaatatt taattatctt ttcaaagaaa agtatggtga agtgaatggg taaaaatatt
     1261 tatttaccga tagatacaaa atctttatat tacaacattc ataatgataa taaaactgga
     1321 catacatgta ctgttgcgac gcctattaaa caagggaaaa gtgttaatag tgagctggct
     1381 aagatttacg atatacctaa aagcatcgat cttgattatg atgtagagtt ggttgactat
     1441 ttaaataaat catcaaaatc taccactaga atcgacaaag catttatttt taacaaatac
     1501 acagtaaatg gtatacatat taattgtgat gcagattatt gtatatttat taaagaagaa
     1561 atcgattcta ataaaaagca attcggaagg ttaaaattgc attaccctat ctctgccttt
     1621 tatgatggaa agtactcttt tgataataaa aaagtaataa atgcaatatc taataagctt
     1681 aacaattttg cttttattgt tcgtggcttt gaatattcat ttgatacaga tatattaaat
     1741 tttgatgttg atgttattgg atataataat attccttatt caaaagtttt cataaacaac
     1801 aagggtgttg gcaacaagca tatagctaat tatgttgata tttatgaaaa ctatgattat
     1861 gaagtaatgg gacttcgtga aaagtttaac aaggaaatta ttaacacgaa gaattatttt
     1921 gaattatatc atatagctaa gattgaagca ttaaacattg ttgcagagta tcttaatgta
     1981 aaaaaagact ttttatctat tagctatccg tactcatttt acgatttatc gacttataaa
     2041 aataatgtaa aaaaatatat tattgtgttt ttcacaactg cgaaagaatt atactttaac
     2101 ataagtatga aacaattatc gttctttaat atgtttaaga attatataga gatatataat
     2161 gtaacaaatc tgtttaataa ttacaaaata aatagatttt cgtatgagga tttaacaaaa
     2221 tttcataaaa ctatatcaac catagttttg agatagtagg cgttattatg gcaaaaacaa
     2281 ttcaattata tccaccagca aaaaaactta taaatagtat gagatgcatg ggatatagct
     2341 tctcaacaag tttagctgac attattgata atagtatttc ggcaaatgca gatagagtcg
     2401 atgtaattta tgatacttta tgtgattttc catatgttca aattattgat aatggtatag
     2461 gtatgtcata tgatgagtta gttaatgcgc ttgtattcgg tactgagaga gactattctg
     2521 aaaatgaatt tggaagatat ggcttaggac taaaagttgc atctttggca caatgtcgta
     2581 agcttactgt ttgctcaaaa aaacaaggca aaataaattg tttaggatat aatttggatg
     2641 ttatagaaaa aacaaataat tgggatgtac tcgtttattc atatagtgag ataaaaagta
     2701 tacctaatat tgaaaaatta aacgaggtat cttctggaac tatagttatg tggcaagact
     2761 ttgacttttt gagagcagaa tcatcagaaa aaagttttaa agataattta agaaagaatg
     2821 ttgttgatgc acgtaatcac gttgctttag ttttccatag aattgagcaa actaaaatat
     2881 attttaatga aaacctaatt cctaagcgag atccattttt attagattct aaaccaagac
     2941 agataactgg aattgtatca acaatagatg tgtttgattc aaagattatg gttactccac
     3001 acactctacc taatgctaat acattaacta gtgaagaaag agaacttctc ggaataaaga
     3061 atggtgtatc tatttatgat gggcaaggtt tttacattta tagaaataaa agattaatta
     3121 tatggggaag ttggctgaga atgaattctc gtagcgaatt caataaattg gcgagaattc
     3181 aaatcgattt accttcatcg ttagattcta tatgggaatt agacgttaaa aaatctactg
     3241 ctaaaatacc agaaatcatt aaagaaaaac tcaaagcaac gattgtagac tctatgggaa
     3301 agagtagaag agtcatcgtt atgcctggca aagtagaaga agctgcacct aataaaatat
     3361 ggttaaggag agagtttggt aaagaacaag ttaactatga attaaataga gaaagttcgt
     3421 tatttaaaaa gatttatgat gaaattggct cctctttgtt gccttatttt gaagcgtatc
     3481 ttaagcaaat tgagaaaaac ttgcctaaat ttaaaatcaa agacgatatt gaggaaaatt
     3541 tcaagttttt aaacaatgtt gctgaaactc gagatgactt gaaaaaagaa ttgattgaaa
     3601 tattgcgtga cgttaattct aataataagg aagaattatt aaaatatctt ataaatcaaa
     3661 aaagatatga gattctaaag gatgatttag aatcaattat gaaggagatt aataatggag
     3721 gaatttaaga accttttata taatatgctt cgtgttgttt ttaaaggtaa agatgctcag
     3781 cattctgaat taatcaaaca agcgaaaatc atctcaaaga atatcgaaaa aaacattttg
     3841 catatttctt taagtcctga agataaagaa gctgctattg atatggtaat taatcaatac
     3901 gagatgaata tcggagttgt tcagccaaat ccttcttttc tatctgatgg agaacctggt
     3961 gaatggttcc ttaatgaaaa ggataataat agttttgaat tcttttcaag atattgtgaa
     4021 tatttgcgag acgatgcaca gttttctgat tcaacaatag ataaaattaa actatcgact
     4081 gaaagcatat tgagttattg tgctaatcct aagggtagaa cgagcaaaga aaaccaaaag
     4141 aagaggggat tggtcgttgg agatgttcaa tcgggaaaaa ctgctaacta tatagcgtta
     4201 atcaatatgg cttgtgatgt tggatataac atagttgttt tgcttgctgg tatgacttca
     4261 atacttagac aacagactca ggatcgtata gatagcggat tcattggcgc taaaagtgat
     4321 actatatcta acattcctga atttgttggt gttggcgttg aattaaaacg ctatttcggt
     4381 attcctttga caaatcataa aaatgatttc gttaaatatg ttaaagaaaa tcaaaatgcg
     4441 ggcgcacaag attttaataa accaattgtg cttgttgtta aaaaaaataa atcgattttg
     4501 aattctgttt ctcaatggct aaaaccagga ttgaataata ttcagacaag taacatttta
     4561 attattgatg atgaagctga taacgcatct gtgaatacta gaaaggatat aaacgaaata
     4621 tcagcaatca atgagtgtat aaggaagata tataataact ttccaattgc gtcttatgtt
     4681 ggctacacag caacgccttt tgcaaatatc tttattaatc cttttgagag ttctcttgaa
     4741 aatcaagatc tattcccagc taattttatc gaattgctag aaactccatc taattatttt
     4801 ggctttgata aggtttttga aaaagatagc aatgataatt atttgcatat tcaattaatc
     4861 aacgaaagtg atgtttataa tataccagct ttgcataagc gtgattatat tgttaaggat
     4921 ttgccagatg acttaataga gtcaatttat cacttttatc tagttaattg tattagaact
     4981 ctccgaggtg atacaaaaaa acatagaagt atgttaataa atgtaacaag atttaatgat
     5041 gtacaatatg aattgtttga tttagttgaa agatatcaca ataattttgt taatttgtta
     5101 gaagagatta acagcaaaat atctagaaaa cctggtattt gttccgtatc tcagaaatta
     5161 tttgagcttt atctaaaaga taaaactttt tcaaaagcaa gaagagagtt ttcttggaag
     5221 gacatatgca gagttttatt acatgaagct aaacaaattg ttatcagagt aattaataac
     5281 aaaactaaga aaacagatag atttaattac gatgattata aggatgatgg tgccagagca
     5341 attataattg gaggctttgt gttatcaaga ggtcttacat gagaaggctt agtcaccaga
     5401 tagtt
//
LOCUS       DAOSGS010000052         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_13445_length_5397_cov_3.949644, whole genome shotgun sequence.
ACCESSION   DAOSGS010000052 DAOSGS010000000
VERSION     DAOSGS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13445_length_5397_cov_3.949644"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLQ22_02900"
     CDS             <1..810
                     /locus_tag="PLQ22_02900"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOH58941.1"
                     /translation="QKFNIETDILRLEDVKELKDDYDLIGISYPIHAFSAPRFVEKRI
                     KELKIKNKHYFIIKTSGELYQLNSTSSYYLYKLMNRNNNIFLSEYHVLMPYNIIFRFD
                     DAITSRILKANRNFIKKIAYETSNKRYKRIDFKTRYKLIRNIFKIQRLGAYLNSFLYK
                     VNLDKCTKCMKCVNSCPTNNIKYENNKFKFSTNCIMCMRCSFFCPEDAIRIGFLNSWK
                     VNGKYDFEILKHDNLQYESNERDSFYNNFLPYLEEHEEPFVLDYEIQKDHS"
     gene            complement(826..1614)
                     /locus_tag="PLQ22_02905"
     CDS             complement(826..1614)
                     /locus_tag="PLQ22_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH58942.1"
                     /translation="MFSKSLKADFYRLKKDNLLWISVIVVTGFSVFTFLIYLLMHNLT
                     TADDLMMPPIITTLSGLRLAMNMTNNAGLIVLIMTIVLMAKDFRQSTIRNKMIIGIKR
                     REAYFSSLIVYYLFVLGIITYNVVLTVLLFSTTFESGVSEGSVILEFLKNYAVGVAIL
                     TPWVIIAHFLTYVTKSLGLALGLTIGLFFLLQIVALIPQIVEVSETTGLLMHFNPVLQ
                     TMSYNMGGFFEEKKYLWFSLGSNAVIPGLLIFIGSFLFSKADIK"
     gene            complement(1607..2521)
                     /locus_tag="PLQ22_02910"
     CDS             complement(1607..2521)
                     /locus_tag="PLQ22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH58943.1"
                     /translation="MNVVLKTNNLTKRYKELKALDDVSLTIYEGDIYGFVGENGAGKT
                     TLIRAVSGLIFPSSGTYEIAGVDYKDSEIAKVRAKMAAIVESPALYLNMSAYDNMLMQ
                     AELIGDVDAKEIDEILNDVGLGYLINSKKKAKNFSLGMKQRLAIAMALLNKPRFLILD
                     EPMNGLDPEGIIEVRNLILKLNKADKITFLISSHILDELAKVCTRYGFIHKGKLIQEI
                     SARDIDVVSEEHLKIKVNDNQKGYELLKKDYEKVELREDYLYVFGKYEVNAIIEKLHK
                     EKLIILEISTIKPSIEDIYLKVIGGKNV"
     gene            complement(2621..3295)
                     /locus_tag="PLQ22_02915"
     CDS             complement(2621..3295)
                     /locus_tag="PLQ22_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58944.1"
                     /translation="MNKNKKILGLFTVSLIVSISLLIYAVYQFVNNVSHNNAYIWLGL
                     GNLLANLIFILVSVLLFLKEEIFLGHLVLLFKMIFIPTFTSAKSLLQGNPTFDLFFII
                     LFLISTVCMFFGLKMKPVVVRHIRWLSYSGILLAFVYESIVYNVPQGLYTNIPNFVLI
                     STHRRESKILFTSVFIRYPFILIDALVNNPNQPVFFYLATIIGILFFIYTLINLFLKP
                     SRFPKS"
     gene            complement(3319..4602)
                     /locus_tag="PLQ22_02920"
     CDS             complement(3319..4602)
                     /locus_tag="PLQ22_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOH58945.1"
                     /translation="MKELLKKLSFLNGKDLADFFKKFKLMAPKALRVDVLREAMAPKV
                     EEQLANTSGGFGIGGQNNYRLLWFAKLSEHQLEKYLSTFDDAELDNKYHNLLVEKMLA
                     YAAEKKVKKADMEELVAASEDNYRRVGNARLDMEEFNNSLDAVFYDEKSCCDGLSVSE
                     FRGVLFNVGTREELFEIAEKYEVKVPERLNKDELFAYCVRQMKIEKYYTAEAEAALSE
                     MTAKDLREWAKNHNIQSGSQLNKKDLIEYILSDYSKTKEDYEVPKDDSVYEMAIPLPY
                     GQEEVDVEALEAKIAELTAEKEKLEKAAAKSTRENNRNKKKIDKQDKEIAKLLALIAD
                     KEKEYEELKAAKAKVKEDKAADKKQDEAIAKLKEEIKALQIELAVVKAKEDEGRELTE
                     EELKEARRNFALDIIWLIFFVLVLAFLMYAIFSML"
     gene            complement(4770..>5397)
                     /locus_tag="PLQ22_02925"
     CDS             complement(4770..>5397)
                     /locus_tag="PLQ22_02925"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH58946.1"
                     /translation="AKKIDFKGKKNVILRDSDLNELVGNLISLYGYQAVFSKATDKKI
                     GDVIQDAKRGYDKLTVTLMPGSKADRFDGDGVLLEKTVILDKGVLASYFGNNQYAQYL
                     GLKPTGNMRFRKLEKGKKSSAELKREPHIEIISLSGIQVDPYADYIGGEVRLANYFDG
                     EKSYPIFGFSFSGSLTESLKEISLSKETKKTTSYEMPSYALLKDLNIL"
BASE COUNT         1874 a          958 c          609 g         1956 t
ORIGIN      
        1 caaaagttta atatagaaac tgatatttta cgtttagaag atgtgaaaga attaaaggat
       61 gactatgatt taataggaat cagttaccct atccatgctt tttctgcacc aaggtttgta
      121 gaaaagagaa ttaaggaact aaagattaaa aacaagcact actttatcat caaaactagt
      181 ggtgagttat atcaactaaa tagtacttct agctactatc tttataaact aatgaataga
      241 aataacaaca tatttctttc tgaataccac gtcttaatgc cttataatat tatcttcaga
      301 tttgacgatg ctattacctc aaggattctt aaagctaatc gtaattttat taaaaaaatt
      361 gcttatgaaa caagcaataa aagatataaa agaattgact ttaagacaag atataaacta
      421 ataagaaaca tctttaaaat ccaaagacta ggagcttacc ttaactcatt tctctataaa
      481 gtcaatttag ataaatgcac taaatgtatg aaatgtgtta atagctgccc aactaataat
      541 atcaaatacg aaaataacaa atttaaattt agtactaatt gtattatgtg tatgcgatgt
      601 tcattcttct gtccagaaga tgcaattaga attggctttc taaatagttg gaaagttaat
      661 ggaaaatatg actttgaaat actaaagcac gataacctcc aatacgaaag taatgaaaga
      721 gactccttct ataataattt cctcccttat ttagaagaac atgaagaacc cttcgtacta
      781 gactatgaaa ttcaaaaaga ccacagttag ctgtggtctt tttccttatt taatatctgc
      841 tttactaaac aaaaacgatc ctataaatat taataatcca ggaataactg catttgatcc
      901 taaactaaac caaagatatt ttttctcttc aaaaaaacct cccatattat aagacatcgt
      961 ctgaagtact ggattaaaat gcattaaaag tccagttgtt tcacttacct caactatttg
     1021 aggaattaag gcaacaatct gtaataagaa aaataatccg attgttaatc ccaaggctaa
     1081 gcctaatgat ttagttacat aggttaagaa atgagctata attacccatg gagttaaaat
     1141 cgcaacccca actgcataat ttttcaaaaa ctctaaaatt acactacctt cactaactcc
     1201 actttcaaaa gttgtagaga aaagtaagac tgttaaaacg acattgtaag ttataatccc
     1261 aagaacaaac aaataatata ctattaaact agaaaaatat gcttctcttc tttttattcc
     1321 aataatcatc ttatttctaa ttgtactttg cctaaaatct tttgccatta agactattgt
     1381 cattattaaa acaattaaac cagcattatt agtcatattc attgccaatc ttaaaccaga
     1441 aagtgttgtt attattggtg gcatcatcag atcatcagca gtagttaaat tatgcattaa
     1501 taaatagatt aaaaaagtaa aaacggaaaa tccagtaact acaattacag aaatccaaag
     1561 taaattatct ttttttaagc gataaaaatc agcttttagt gatttactaa acatttttgc
     1621 ctcctattac ctttaaataa atatcttcaa tactaggctt aatagttgaa atttccagaa
     1681 taattaattt ttctttatgc aatttttcaa taatcgcatt tacttcatac tttccaaaaa
     1741 cataaagata gtcttctctt aactctactt tttcataatc tttctttaat aactcatatc
     1801 ctttttgatt atcattaact ttaatcttta aatgctcttc agaaacaaca tcaatatctc
     1861 tagcactaat ttcttgaatt aactttcctt tatgaataaa tccatatcta gtacatactt
     1921 tagctaattc atctaaaatg tgtgaagaaa ttaaaaatgt aatcttatca gctttattaa
     1981 gttttaaaat taaattacga acctcaataa taccttcagg atctaaacca ttcatcggtt
     2041 catctaaaat caaaaatctt ggtttattta ataatgccat cgcaatcgct aatctttgtt
     2101 tcattcctaa agaaaagttt ttagctttct ttttagaatt gattaaataa cctaaaccaa
     2161 catcatttaa aatctcatca atttcttttg catcaacatc accaataagt tcagcttgca
     2221 tcagcatatt atcataagca ctcatgttta aatataatgc tggtgactct acaatcgcag
     2281 ccatctttgc tctaacttta gcaatttctg aatctttata atctactccc gctatctcat
     2341 aagtccctga actaggaaag attaacccgg aaactgctct aattaaagtt gttttacctg
     2401 caccattttc cccaacaaac ccataaatat caccttcata gattgttaaa ctaacatcat
     2461 ctaaggcttt taattcttta taacgttttg ttagattgtt tgtttttaat actacattca
     2521 tctctctata atctcctatg ctttaaaatt ataacacgca gttcttaaaa atatattaaa
     2581 aagagttagt ttttactaac tcttttcttt atctttatat ttaacttttt ggaaatctac
     2641 tcggctttaa aaacaaattt attaatgtat aaatgaagaa taatattccg ataatcgtag
     2701 ctaaatagaa aaatactggt tgatttggat tatttactaa agcatcaatt agaataaatg
     2761 gatatctaat aaataccgaa gtaaagagga tttttgattc acgtctatga gtacttatca
     2821 aaacaaagtt gggaatgttt gtatataaac cttgtggaac attataaacg atactttcat
     2881 aaacgaaagc aagcaaaatc cctgagtacg ataaccatct aatatgcctt actacaaccg
     2941 gcttcatctt taaaccaaaa aacatacaaa cagtagaaat caaaaacaga attataaaga
     3001 ataaatcaaa tgttggattt ccttgaagta agcttttggc gctagtaaac gttggaataa
     3061 agatcatctt gaataacaaa actaaatgac ctaaaaatat ttcttctttt aaaaataaaa
     3121 gcacactaac taaaataaat ataagattag ctaataaatt tccaagtccc aaccaaatat
     3181 aagcattatt atgggacacg ttatttacaa actgatatac tgcgtaaatc aataaggaga
     3241 ttgatacgat gagactaaca gtaaataacc caaggatctt tttattctta ttcatattac
     3301 tctcccctta tctacttttt atagcattga gaaaattgcg tacattaaga atgctagtac
     3361 taaaacaaag aatattaacc aaataatgtc taaagcaaaa ttacgtcttg cttcttttag
     3421 ttcttcttca gttaattctc ttccttcatc ttcttttgct tttactacgg caagttcaat
     3481 ttgtaatgct ttgatttctt ctttaagctt agcaattgct tcatcttgtt ttttgtcagc
     3541 agctttgtct tctttaactt ttgcttttgc agcttttaat tcttcatatt ctttttcttt
     3601 atctgcgatt aatgcaagta gtttagcgat ttctttgtct tgcttatcaa tctttttctt
     3661 gttgcgattg ttttcacgag tacttttcgc tgctgctttt tctaattttt ctttttcagc
     3721 tgttaattct gcaatcttag cttctaatgc ttcaacatca acttcttctt gaccataagg
     3781 tagtggaata gccatttcgt atactgaatc atcttttgga acttcgtagt cttctttagt
     3841 tttgctgtaa tcagataaga tgtattcaat taaatctttc ttgtttaatt gacttccact
     3901 ttgaatgtta tggtttttag cccattcacg taaatcttta gcagtcattt ctgataatgc
     3961 agcttctgct tcagctgtgt agtatttttc aattttcatt tgacgtacac agtaagcaaa
     4021 taattcatct ttgtttagac gttctggaac tttaacttca tacttttcag cgatttcaaa
     4081 taattcttct ctagttccta cattaaataa aacgcctcta aattcactta ctgataagcc
     4141 atcacagcag cttttctcat cataaaatac tgcatctaaa gaattattaa attcttccat
     4201 gtctagacga gcattgccta cacgacgata gttatcttca ctagcagcta ctaattcttc
     4261 catatctgct tttttaactt tcttctcagc cgcatatgct aacatcttct ccacaagtaa
     4321 attgtggtat ttgttatcaa gttctgcatc atcaaacgta cttaaatatt tttctaattg
     4381 atgttctgat aattttgcga accacaacaa cctataattg ttttgacctc cgataccgaa
     4441 gccacctgat gtattagcta attgttcctc aacttttggt gccatagctt cacgtagaac
     4501 atctacacgt aaagccttag gtgccataag tttaaatttc ttaaagaaat cagctaaatc
     4561 tttaccattc aaaaatgata atttttttaa taattccttc atttttatac ctaccttttc
     4621 gtatctttat tttaatacac tttttatact ttgtcaacca tttatccgtt tttttataaa
     4681 atatacgcta tattttcaaa aataagtcaa aatttcaact tgcctcaatt aaaaaacgca
     4741 aaacctaaat tttgcgtttc tttcttattt taaagtatat ttaaatcttt taaaagagca
     4801 taactaggca tttcataact agttgttttc ttagtttctt ttgaaaggga aatctctttt
     4861 aaactttcag ttaaactacc tgaaaaagaa aaaccaaaga ttggataact cttttcacca
     4921 tcaaagtaat ttgctagtct aacttcacca ccgatatagt cagcataagg atctacttga
     4981 ataccactta aagaaataat ttcaatatgt ggttctcgtt ttaactctgc acttgatttc
     5041 ttaccttttt ctaactttct aaatcgcata ttaccagtag gttttaatcc taagtattga
     5101 gcatattggt tattaccaaa ataactagct aaaactcctt tatctaaaat aacagttttc
     5161 tctaatagta caccatcacc atcaaaacga tcagcttttg atccaggcat taaagttaca
     5221 gttagtttat cataacctct cttagcatct tgaatcacat caccaatctt tttatctgta
     5281 gctttactaa aaacagcttg atagccatat aaactaatta aattaccaac taattcattt
     5341 aaatcactat ctcttaagat aacatttttc ttacctttaa aatcaatttt ctttgct
//
LOCUS       DAOSGS010000053         5390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_13465_length_5390_cov_4.826804, whole genome shotgun sequence.
ACCESSION   DAOSGS010000053 DAOSGS010000000
VERSION     DAOSGS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13465_length_5390_cov_4.826804"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLQ22_02930"
     CDS             <1..183
                     /locus_tag="PLQ22_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58947.1"
                     /translation="GSNQWANPDEIKNQLLFNSTLDYVSGEIFFTYHNLIDTVDKYPE
                     AQKSIKSLWTKEVKSI"
     gene            296..553
                     /locus_tag="PLQ22_02935"
     CDS             296..553
                     /locus_tag="PLQ22_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOH58948.1"
                     /translation="MIVELRKKAQITLPKKIVDQLNLYEGDKFEISVKNGIIMLEPVA
                     IYPKEYINKLAKEIKLLKADNKYEDKTFDKVDDLIKHLEKK"
     gene            695..2551
                     /locus_tag="PLQ22_02940"
     CDS             695..2551
                     /locus_tag="PLQ22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH58949.1"
                     /translation="MYKIYPKVKYIKYTKDEFTKKDKYSVFFTENLENVFTNLKRFIK
                     VKKTRKVGDINFIYEKARSVQTYKLVIEKDKIDVYYNDASGAYYASQTLKQIFKSEKL
                     ETVEIWDEPKLLVRGFTLDISRNKVPSLKTIYKIIDLMAELKMNHFELYVEGFSYEYD
                     FARKYLLEDGYITKEEYKFIEDYCFAREIDFVPNQNGFGHMGPWLATKEYKDLAICPD
                     GAFYWGRHRSPVTLDPKDPRSIKLVSKMYEEMLPNRKSKYFNINFDEPMELGMGKTKE
                     LVEKEGLSNVYLDYLNLVLEEVKKYNKTPIMWGDVLIKDQEALKKLPKDIIFVDWGYD
                     ATYKFSETLKTLGTLNIKFGAAPGTTSWCSFTGRTQDFIENIKSACDNVYKYNGLGIL
                     LTDWGDAGHLQLLPSIVAPLVFAGFYSWSLQEGEMLNVTKYLNEEVFQDEKGNFAQIL
                     LGIGNYYQFENYYITNATLTFFGYMWADYALKQEKPLDYYYEKIKNSVIEDVKYKALV
                     EYLELYRKGLKNTKLKIKDKKLVLAEINQSIDLVLVTRLLMQSFNPKLDKNNKLKLLE
                     KLFKNAKILEKQKELWLARNKKSNLAESIEMLEAVFKLGKLHYKALQGENHE"
     gene            2544..>5390
                     /locus_tag="PLQ22_02945"
     CDS             2544..>5390
                     /locus_tag="PLQ22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH58950.1"
                     /translation="MSKNKKKSQEKVEDVQQVEQQKEAVAIEDVKITTVNYDLVDSSE
                     SRLKRRTNRVKMIKRAIWLTILVLIMTILVVSIVKSFRPDRTTSDSTIPEELYPEGDY
                     NNYLRAKATGITDPQRLEVLDFDKDIKPLLVMPQIESIAVNLATGVASPGSIDFKYID
                     ADDPYSSIVLPNGETRAGYYTSETGSVTFPVDVPEAGFYYLSLVYYAEKSRDEIFNVR
                     ETSGGANIERRVLINGKLPFDSAGHIRFYRMWKDRAAIVQDINGNDMKPSQMEVFDLS
                     TAYVRDYTGYVIEPYMFHFKSGANTITFESVRENMSIVELKLESVPAIVTYQDYLNLH
                     QDKSAPSEVMLQVEAEDSTKRSSPTLYAIADRTSPTNTPSHPVKVTLNSIGGTKWTAP
                     GDWIEWEVDVEEAGMYELSFKAKQNTARGLFSTRRLYINGEIPFIEANSSNFNYSTNW
                     RLVTMGNSEENYKFYFHEGINTIRLESTLGAYGSPINKVRNVVNSLNNMYLQIISITT
                     VNPDPYQEYHLYGDNARIVGLLECFEANAQELREVSAYITELSGEKGSLVSVLDTMAI
                     QLESFLEKPRTIQERLGTFVQNLSSLGTWITSIQEQSLTVEALYVSSSDAELPKPNAD
                     WFSNVWFDIKAFFMTFFFDYQSIGVTNAEGFDKEIEVWFLTSAVAGREQANAMKTLCD
                     TTFVNQKGINVILKVVDPGVLLPATLAGTGPDVALNVYNGLPVNYALRNAAYDIAQFD
                     DFRYEVTEYGVKVWLDGEANPRFADSAMTPYALQKTADIHDLEFYALPITQSFLVTFY
                     RKDIFQERGWTVPKTWDEVTSLVTELQISNLQFYLPLSAVGASAAVNQVFSSMLFQNG
                     GNFYTEDKTATALDSEEAMQSFETWVKYYTDYSFPTAASFLNRFRTGETPIGVVDYTI
                     FNYLAVFAPEITGKWGFAPIPGVRNPETGIINNKG"
BASE COUNT         2023 a          670 c          984 g         1713 t
ORIGIN      
        1 ggtagtaatc aatgggcaaa cccagatgaa atcaaaaacc aattgttatt taacagcact
       61 ttagattatg tttctgggga aatttttttc acttatcaca acttaattga tacagtagac
      121 aaatatcctg aggctcaaaa gtcaattaaa agtctatgga caaaagaagt aaaaagtatt
      181 taaaaagagc ataaaatgct ctttttattt taaataattg ttgcaaattg tagttgtttg
      241 tattacacta ttagtaggaa agtaggaatt tccaacaaca aaaggaggaa taaatatgat
      301 tgttgaatta agaaaaaaag cgcaaattac attgccaaag aaaattgtag atcaattaaa
      361 tttatatgaa ggtgataagt ttgaaatatc tgtaaaaaat ggaattataa tgcttgaacc
      421 agttgctatt tatcctaagg aatatattaa taagttagca aaagaaatta aattattaaa
      481 agctgacaac aaatacgaag ataaaacatt tgataaggta gacgatttaa taaaacattt
      541 agaaaaaaaa tagatgtttc aaaatcgact tatttcaatt taaaagagca taaaatgctc
      601 tttttttatt caatttgccc ttgaaaaaaa atgggttttg tagtataatt ttgttatata
      661 gtatataaac tcaaaacgaa aggaatgatt gaaattgtat aagatttacc caaaagtaaa
      721 gtatattaaa tatacgaaag atgaatttac aaagaaagac aagtattccg ttttttttac
      781 tgaaaattta gaaaacgttt ttacaaatct aaagaggttt attaaagtaa agaaaactag
      841 gaaagttggg gatattaatt ttatctatga aaaggcaagg tcggttcaaa cttataaact
      901 tgttattgaa aaagataaaa ttgatgttta ttacaatgat gctagtggtg cttattatgc
      961 aagccaaact ttaaagcaaa tttttaaatc tgaaaagtta gaaactgtag agatttggga
     1021 tgaacctaaa ttattagtta gaggatttac tttagatatt agtcgtaata aagtgccttc
     1081 attaaagact atctataaaa tcattgatct tatggcagag cttaagatga accattttga
     1141 gttatatgtg gaaggtttta gttatgagta tgactttgca agaaagtatt tattggaaga
     1201 tggttatatt acgaaagaag aatataagtt cattgaagat tactgttttg caagagaaat
     1261 agattttgtt cctaaccaaa atggttttgg tcatatggga ccatggctag ctactaaaga
     1321 atataaagat ttagctattt gtcctgatgg agcattttat tggggaagac atagaagccc
     1381 agtaacatta gatccaaagg atccaagatc aattaaactt gtaagtaaga tgtatgaaga
     1441 gatgcttcct aatagaaaat ctaaatattt caatattaac tttgatgaac caatggaact
     1501 tggtatgggt aagactaagg aacttgtgga gaaggaaggt ttaagtaatg tttatttaga
     1561 ttatttaaat cttgttttag aagaagttaa aaaatataat aaaacaccga ttatgtgggg
     1621 ggatgtttta atcaaagatc aagaagcttt gaagaaacta cctaaagata ttatctttgt
     1681 agattgggga tatgatgcaa cttataagtt ttctgaaaca ttaaaaactt taggaacttt
     1741 aaacattaag tttggagctg ctcctggtac tacaagttgg tgtagtttta caggaagaac
     1801 acaagatttt attgaaaata ttaaaagtgc ttgtgacaat gtttataaat ataacggttt
     1861 aggtatttta ctaactgatt ggggtgatgc aggtcacttg caattacttc catcaattgt
     1921 agcaccttta gtatttgcag ggttttattc ttggagtttg caagaaggtg aaatgcttaa
     1981 tgtaactaag tatttaaacg aagaagtatt ccaagatgaa aaaggaaatt ttgcacaaat
     2041 attacttggt attggtaatt attatcaatt tgaaaactac tatattacaa acgctacttt
     2101 aactttcttt ggttatatgt gggcagatta tgctttaaag caagaaaagc cattagatta
     2161 ttactatgag aaaattaaaa acagtgttat tgaggatgtt aaatataaag cattagtaga
     2221 gtatttagaa ctatatcgca aaggattaaa aaatactaaa ttaaagatta aagacaagaa
     2281 attagtttta gctgaaatta atcaaagtat tgacttagtt ttagttacaa gattattaat
     2341 gcaaagtttt aatcctaaat tagataaaaa caataaactt aaattattag aaaaattatt
     2401 taagaatgca aagattttag aaaaacaaaa agaattatgg ctagctagaa ataagaaaag
     2461 caatttagct gagtcaattg agatgttaga agctgtcttt aaattaggta agcttcacta
     2521 caaagcatta caaggagaaa accatgagta aaaataagaa aaaatctcaa gaaaaagttg
     2581 aagatgttca acaagtagaa caacaaaaag aagcagtagc aattgaagat gttaagataa
     2641 ctactgtgaa ctatgattta gttgattcaa gcgaaagtag gttaaagcgt agaactaatc
     2701 gtgtgaagat gattaaacga gcaatttggc ttacaatttt agttttgatt atgactattt
     2761 tagtagtttc aattgtgaag agttttaggc ctgatagaac aacatctgat tcaacaattc
     2821 cagaagagtt atatccagaa ggtgattaca ataattactt aagagctaaa gctacaggta
     2881 ttactgatcc tcaaaggtta gaagtacttg actttgataa agatattaaa ccattacttg
     2941 taatgccaca aatcgaaagt atagcagtta atttagctac tggagttgca tcaccaggat
     3001 caattgattt taagtatata gatgcagatg atccatatag ttcaattgtt ttaccaaatg
     3061 gagaaacaag agctggatat tatacatcag aaacaggaag tgttactttc cctgttgacg
     3121 ttccagaggc tggattctac tatttgagtt tagtatatta cgcagagaaa tcacgtgatg
     3181 aaattttcaa tgttagagaa actagtggtg gagctaatat tgaaagaaga gttttaatta
     3241 atggcaaatt accatttgat agtgccggac acattagatt ttatagaatg tggaaagata
     3301 gagcagcaat tgttcaagat attaatggta atgatatgaa accatcgcaa atggaagtat
     3361 tcgatttaag tacagcttat gtaagagatt acactggtta tgtaatagaa ccttatatgt
     3421 tccattttaa atcaggagct aatacaatta cttttgagtc tgttagagaa aatatgagta
     3481 ttgtagagtt aaaactagaa tctgttccag caatcgttac ttatcaagat tatttaaatc
     3541 tacatcaaga taaaagtgca ccaagtgaag taatgttaca agttgaagct gaagattcaa
     3601 caaaacgttc atcgccaaca ctatatgcaa ttgctgatcg tacttctcca acaaacactc
     3661 catcgcatcc agttaaagta acactaaact caattggtgg tacaaaatgg acagcacctg
     3721 gagattggat tgaatgggaa gttgatgtag aagaagcggg aatgtatgaa cttagtttta
     3781 aggctaaaca aaatacagct cgtggacttt tctctacaag aagattatat attaatggtg
     3841 aaattccatt tatcgaagct aatagttcta attttaatta cagtacaaat tggcgattag
     3901 ttacgatggg aaatagtgaa gaaaattata aattctattt ccatgaaggt attaatacaa
     3961 ttagactaga atctacactt ggggcttacg gtagtcctat aaacaaagtt agaaatgtag
     4021 ttaatagttt aaataatatg tatttgcaaa ttatttcaat aactacagta aacccagacc
     4081 catatcaaga atatcactta tatggagaca atgcgcgtat tgttggttta ctagaatgtt
     4141 ttgaagcaaa tgctcaagaa ttaagagaag tgtcagccta tattactgaa ctttcaggag
     4201 aaaaaggaag tttagtatca gtactagata caatggcaat tcagcttgaa tcatttttag
     4261 aaaaaccaag aacaattcaa gaaagattag gaacgtttgt tcaaaactta tcttcacttg
     4321 gaacttggat tactagtatt caagaacaat cactaacagt tgaagcttta tatgtttcta
     4381 gtagtgatgc agaattacca aaaccaaatg ccgactggtt tagtaatgtg tggtttgata
     4441 ttaaagcttt ctttatgact ttcttctttg attatcaatc aattggagta acaaacgctg
     4501 aaggttttga taaagaaatt gaagtatggt ttttaactag tgctgttgcc ggtagggaac
     4561 aagctaacgc aatgaagact ttgtgtgata caacatttgt taatcaaaaa ggaattaacg
     4621 ttattttaaa ggttgtagat cctggagtat tattaccggc tacacttgct ggtacaggtc
     4681 cagatgtagc cttgaacgtt tataacggat tacctgtaaa ctatgcttta agaaatgctg
     4741 cttatgatat tgcccaattt gatgacttcc gatatgaagt tactgaatat ggtgttaagg
     4801 tatggttaga tggtgaagct aatccaagat ttgctgatag tgcaatgaca ccttatgctt
     4861 tacaaaagac agcagatatt catgatttag agttttatgc tttaccaatc actcaatctt
     4921 tcttagttac tttctatcgt aaagatattt tccaagaaag aggttggaca gtacctaaaa
     4981 cttgggatga agttacaagc ttagtaacag agttacaaat ttctaactta cagttctatt
     5041 taccactaag tgcagttgga gcatcagcag cagtaaacca agtgttctca tcaatgctat
     5101 tccaaaatgg tggtaatttc tatactgagg ataaaacagc tactgccttg gatagcgaag
     5161 aagcaatgca atcatttgaa acttgggtta agtattatac agattattct ttcccaacag
     5221 ctgcaagctt cttaaataga tttagaactg gtgaaactcc aattggagtt gtagattata
     5281 caattttcaa ctacttagct gtattcgcac cagagattac tggtaaatgg ggctttgcac
     5341 cgattccagg tgtgagaaat ccagaaacag gcattatcaa taacaaaggt
//
LOCUS       DAOSGS010000054         5370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_13543_length_5370_cov_6.097084, whole genome shotgun sequence.
ACCESSION   DAOSGS010000054 DAOSGS010000000
VERSION     DAOSGS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13543_length_5370_cov_6.097084"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ22_02950"
     CDS             <1..295
                     /locus_tag="PLQ22_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58951.1"
                     /translation="SWISVTWYVLLPLYYTTTALAKTAYLDTGIWKYGTTGLCGGGDV
                     YSKYSDWDHTYSYASVKNGKGKTASDTKKGAQASASLTASKFRTDYSYYNFWD"
     gene            385..2406
                     /locus_tag="PLQ22_02955"
     CDS             385..2406
                     /locus_tag="PLQ22_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58952.1"
                     /translation="MNKRTIMFCIFTVNLLLFLSYNIIKFSTYPNEQIGLNKECFTVS
                     CPDEIEREEYIDILYTISNGISADIMLIHLNDDYTYDFYRTDSDNKFIELEDIDDPSG
                     SYATHPANGEKKINGFITKDNGFRILPMITLKSSDMDLSVQELMVNSEDAKGIIDELL
                     KYGMIIDAERGVSIENDFSSLYLVIIIFMIFLVISEIIYAFSRSNEMIILKTMGFRNN
                     DIVRKECKSTFFFFFIIFIIVILINVIIFSMIFDLKSSLLFLKKISVKSIIYIVSAIA
                     IFGLSILLVSSQCNIRNSKGMKFNRELFYITAGFKSFFLIVLAILITRSASDSLKLYN
                     MFQNTELIINTYSDYACTELNTRLEDPMDYPDKYASKFLEFYRNMNTDNNLVIADFGS
                     LNNIEETKENNIPQVTINSNYIDLTNNIFGVDGIPLDSSMLKKEKINFLVPDGYDVSK
                     LTKINISEEDLNYIYYSSDSRFFTFSNEINISEGGYCCNVIAEIYDPEFEYNHTSSLT
                     FTAFMSSFLSNSMFFKYDLQNQKSAYEQIYPVLNKTEIDKIMFSANSVEELFNENLKQ
                     FKNNFVFEFIQLIIVCGSFIILVIYTTELYYRNYAKDIALKVINGYCFIEIFFNRMIF
                     KAILLPLLIFLRSVSFPVALFCVLSEQIIFVICVRKNIKRSTVSVFKEE"
     gene            2410..3045
                     /locus_tag="PLQ22_02960"
     CDS             2410..3045
                     /locus_tag="PLQ22_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020062193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH58953.1"
                     /translation="MNICAIKAENICKKFGDQEILTNLNLEINDGDFICITGESGKGK
                     TTLLNIMGMLDAPDSGNICIKGVNNPKLRSKVGCRLIKNELFYVFQNYGLVEDKTVKY
                     NLEISAKFSGLNKVSDFEKALTRVGLNPDFLKKKVFTLSGGEQQRCALARMYLKNFNI
                     VLADEPTGSLDPDNRDMILEIFKNINKEGKTVIIVTHDKEVVKCAGKVVKL"
     gene            3018..3476
                     /gene="lepB"
                     /locus_tag="PLQ22_02965"
     CDS             3018..3476
                     /gene="lepB"
                     /locus_tag="PLQ22_02965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH58954.1"
                     /translation="MCRKSRKTLKEFIFILLYAFIIFAFLYNFRIIKVDGVSMEPTLK
                     NKSHILVKRYNDELSVDDIIVFRQENDLCIKRVAAISGDFISLKEGSVCINNVIYNDY
                     KYSGYSSSYIIENDEVFVLGDNSRNSIDSRNFGPIKKNEIKYVKIRKDEN"
     gene            3533..4768
                     /locus_tag="PLQ22_02970"
     CDS             3533..4768
                     /locus_tag="PLQ22_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH58955.1"
                     /translation="MKKNDSNKIKFVGFAINDECNIYIDSFKKENPDIEVEIIDFAEN
                     EVSYDAINRMNASLVSENCGDIIICNSYLKIENLIKNNLISDLYKINYVKEHKDDYIP
                     NIIKCCESDGKLYSIFPSFSIKAFVCKKCNISQDSWNLKKFNNIISEFANEEKNVFGN
                     RSTDCFEKIFNCILNYYFYNDTVEANYDNLLETLDVIKKCNDISKQNQIKSGNIEYLY
                     SDDSQIIAEEEIYDFNRYHVLKKAVFQDDISLLGFPLEKNTLSISPEFEISVLNKSKN
                     NKNVEKFIEFILSDEMQKACVYSESSFPMTINNFNLSCERAMSEIIYDQNGNNLGKKS
                     DKYYIDDKCIDLGIPSANDLSGFKDQIFLVDQIYRIDTNIKNIISDSLLPYWNGDSNA
                     TDTLDKIISKLKIYNSELK"
     gene            4933..>5370
                     /locus_tag="PLQ22_02975"
     CDS             4933..>5370
                     /locus_tag="PLQ22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOH58956.1"
                     /translation="MSKIITQKAEENKQISERIKSFMKRFEVSSALKSSNAVKIKGFA
                     IIEIFQYLFMLVFAHRSIYMDMKRDTVPFAKDTVYRFLNSARINWLKFTTRLSAKIIE
                     EAIDPLTSEQRENVLIIDDSVFERNRSKKVELLTKVFDHAKRRY"
BASE COUNT         1950 a          642 c          906 g         1872 t
ORIGIN      
        1 cagttggatt tcagtaactt ggtacgtcct gttaccatta tattatacca caacagctct
       61 tgctaaaaca gcttaccttg acactggtat ctggaaatat ggtactactg gtttatgcgg
      121 tggcggtgat gtatattcaa agtattcaga ttgggatcat acatattcgt atgcatctgt
      181 aaaaaatgga aagggaaaga ctgcttctga taccaaaaaa ggagcacagg catctgcttc
      241 actaacggca tctaaatttc gcacggatta ttcttactat aatttctggg attaatctga
      301 gctactatta agctaggcat cgatatcagg gattaaatta atgttgaatc ttttggtatc
      361 gatgcttttt taaggagaga attgatgaat aaaagaacta taatgttttg tatttttaca
      421 gtgaatttgt tgctgttttt gtcatataat attattaaat tttcaacata tcctaatgaa
      481 cagattgggt taaataaaga atgcttcaca gtttcctgtc cagatgaaat agagagagaa
      541 gagtatatag atattttgta tactatttca aatgggattt ctgctgatat aatgttaata
      601 catttaaatg atgattatac ttatgatttt tatcgcactg actcagataa taaatttatt
      661 gagcttgaag atattgatga tccgtcagga agttatgcaa ctcatcctgc taatggtgaa
      721 aaaaaaataa atggatttat tacaaaagat aatggattca ggattctccc tatgattacg
      781 ttaaaaagta gcgatatgga tttgtcagtt caggaattaa tggtaaatag tgaggatgcg
      841 aagggaatta tcgatgaact tctaaaatat ggaatgataa ttgatgctga gagaggggta
      901 agcattgaaa atgatttctc gtcactgtat ttagtaataa ttatttttat gatatttctt
      961 gttatttcag aaattattta cgctttttca cgctcgaatg aaatgattat cctaaaaaca
     1021 atgggatttc gcaataatga cattgtaaga aaggaatgta aaagtacttt ctttttcttt
     1081 tttattattt ttattattgt tattttaatt aatgttatta ttttttctat gattttcgat
     1141 cttaaatcat cgcttttatt tttaaagaaa atatctgtaa agagtattat ctatattgtt
     1201 tcagctattg caatttttgg tttatccata ttgcttgtaa gctctcaatg taatatacgc
     1261 aattccaaag gaatgaaatt caatcgtgaa cttttctata taacagctgg atttaaaagt
     1321 ttctttctga tagttttggc gatactgatt actaggtctg cttctgactc acttaagtta
     1381 tataatatgt tccaaaacac tgaattgata ataaatactt attcagatta tgcatgtaca
     1441 gaactgaata cgcgtcttga agacccgatg gattatccag ataagtatgc ttcaaagttt
     1501 ctcgagtttt atcggaatat gaatactgat aataatttag taattgcgga ttttggctca
     1561 cttaataaca ttgaagaaac aaaagagaac aatattccgc aggtaacaat aaatagtaat
     1621 tacattgatt tgacaaataa tatttttggg gttgatggaa taccacttga ttcttcaatg
     1681 cttaaaaaag aaaaaatcaa ttttcttgtt cctgatggtt atgatgtaag taagctcacc
     1741 aaaataaata tttcggaaga agacttgaat tatatttatt attcgtcaga cagtcgcttt
     1801 tttacttttt caaatgaaat aaatatttcg gaaggtggtt attgctgtaa tgtaatcgca
     1861 gaaatatatg atccggaatt tgaatataat catacatcat cattaacatt tacagcgttt
     1921 atgtcatcat tcctttcaaa ttcaatgttt tttaaatacg atcttcaaaa tcagaaaagt
     1981 gcttatgagc agatttatcc tgtacttaat aaaactgaaa tcgacaaaat aatgtttagt
     2041 gcgaatagtg ttgaggagtt atttaacgaa aatcttaagc aattcaaaaa caactttgtt
     2101 tttgaattta tacagttaat aattgtctgc ggatcgttca ttatacttgt tatctataca
     2161 acggaacttt attatagaaa ttatgctaag gatattgctt taaaagtgat taatggttat
     2221 tgcttcattg agatattctt taatagaatg atttttaaag cgatcttatt gccattgctt
     2281 atatttttac gttctgtaag ttttccagtt gcattgttct gtgtgttatc tgaacaaatt
     2341 atatttgtaa tatgtgtaag aaagaatata aaacgaagta cagtatcagt atttaaggag
     2401 gaataaactg tgaatatatg cgctataaaa gcagagaata tatgtaaaaa atttggtgat
     2461 caagaaatac tcaccaattt aaatcttgaa attaatgatg gtgattttat ttgtatcact
     2521 ggagaaagtg gaaaagggaa aacaacactc ttgaatatta tgggtatgct tgatgcacca
     2581 gattccggga atatatgcat taaaggagtt aataatccga aacttcgcag taaagtaggt
     2641 tgtagattaa taaaaaacga attgttttat gtgtttcaga attatggtct tgttgaagat
     2701 aaaacagtta agtacaatct cgagatatca gccaaatttt ctggtcttaa taaagtaagt
     2761 gattttgaaa aagctttaac aagagtaggg cttaatcccg actttcttaa gaaaaaagta
     2821 tttacactta gtggtggaga gcaacagcgt tgtgcgttag caagaatgta tctaaagaat
     2881 tttaatatcg ttcttgctga tgaaccaaca ggtagtcttg atccggacaa tcgtgatatg
     2941 attctggaaa tattcaaaaa tataaataaa gaaggtaaaa cagtaataat tgttactcat
     3001 gataaagagg tagtgaaatg tgcaggaaaa gtcgtaaaac tctaaaagaa ttcattttca
     3061 ttcttttata tgcatttatt atttttgctt ttttgtataa ttttcgaata ataaaagtag
     3121 atggtgtttc aatggaaccc acattaaaaa acaaaagtca tatattagta aaaagatata
     3181 atgatgaatt gtcagttgat gatataattg ttttccggca agaaaatgat ctttgcataa
     3241 aaagagtagc tgcgattagt ggagatttta tatcattaaa ggaaggctcg gtttgcatta
     3301 ataatgttat ttataacgat tataaatata gtggttattc aagtagctat attattgaaa
     3361 atgatgaagt gttcgtttta ggcgataatt caagaaatag tattgacagt cgaaattttg
     3421 gaccgattaa aaaaaatgaa atcaaatacg ttaaaataag gaaagatgag aattgaaaaa
     3481 aaattgtgtt ctattggctt tttttatgtg cctatcgttg acctcgtgtt cggtgaaaaa
     3541 gaacgatagt aataaaataa aatttgtagg atttgctata aatgatgaat gtaatatata
     3601 tattgatagt tttaaaaaag aaaacccaga tattgaagtt gaaattattg actttgcgga
     3661 aaatgaagtt tcatatgatg ctattaatcg tatgaatgct tctcttgtct cagaaaattg
     3721 cggcgatata ataatttgta attcttattt gaaaattgaa aatctaataa aaaacaattt
     3781 gatatcagat ttgtataaaa ttaattatgt taaagagcat aaagatgatt atatccctaa
     3841 tattattaaa tgctgcgaat ctgatggtaa attatacagt atatttcctt ctttttctat
     3901 caaagcgttt gtgtgcaaaa aatgcaatat ttcacaggat tcatggaatt tgaaaaaatt
     3961 taacaatata atctccgaat ttgcgaatga agaaaaaaat gtttttggta atcgttctac
     4021 tgattgtttt gaaaaaatat tcaattgtat tttaaattat tatttttata acgatactgt
     4081 tgaggcaaac tacgataatc ttttagaaac actggatgtc ataaaaaaat gcaacgatat
     4141 atctaaacag aatcaaataa aatctggtaa tattgagtac ttgtattcag atgacagtca
     4201 aataatagct gaagaagaaa tatatgattt taacagatat catgtgctta aaaaggctgt
     4261 ttttcaggac gatatttctt tgcttggttt tccgttagaa aaaaatacgc tgagtatttc
     4321 gccggaattt gaaatatcag tacttaataa atctaaaaat aataaaaatg ttgaaaaatt
     4381 cattgaattt atactttcgg atgaaatgca aaaggcttgt gtatatagtg aatctagttt
     4441 tccaatgact ataaataatt tcaatttgag ctgtgaaagg gcaatgagtg aaattattta
     4501 tgatcaaaac ggcaacaact tagggaaaaa aagtgataaa tattacatag atgataagtg
     4561 tattgattta ggaattccgt cagcaaatga tctttctggt tttaaagatc agattttttt
     4621 ggttgatcag atatatagaa ttgatacaaa tattaaaaat ataatatctg attcactttt
     4681 accttactgg aatggtgact caaatgctac tgatacactt gataagataa ttagtaaatt
     4741 gaaaatttat aattctgaat taaaataata tgaagaattc ttttattgac tcaaacttcg
     4801 cagttaataa actgcgtgtg catcttgaaa attcaataga tagataaaaa aaaatatagt
     4861 atgtagtctc caaaaatgtt ataatgtaag tgataacaaa acattaataa atcacaaagg
     4921 agattacata ctatgtcaaa aattataaca cagaaagcag aagaaaacaa gcagatttca
     4981 gaaagaatta aaagctttat gaaaagattt gaagtttcat ctgcattaaa atcatccaat
     5041 gcggtaaaaa tcaagggatt tgcaataatc gagatatttc agtatctttt tatgcttgta
     5101 tttgcgcatc gttcaatcta catggatatg aaaagagata ctgttccatt tgctaaggat
     5161 accgtctatc gttttcttaa ttcagcccgt ataaactggc ttaagttcac aaccagatta
     5221 tctgctaaaa ttattgaaga agcaatagat cctcttactt cagaacaacg tgaaaatgtc
     5281 ctgataattg atgattcggt ttttgaaagg aaccgttcta aaaaggttga actgctcaca
     5341 aaagtatttg atcatgccaa aagaagatat
//
LOCUS       DAOSGS010000055         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14007_length_5242_cov_6.451128, whole genome shotgun sequence.
ACCESSION   DAOSGS010000055 DAOSGS010000000
VERSION     DAOSGS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14007_length_5242_cov_6.451128"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PLQ22_02980"
     CDS             complement(<1..1541)
                     /locus_tag="PLQ22_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58957.1"
                     /translation="MKKVLKFILVLFLLVTLVGCKDKDEAFKAVESLIDNIGEVTLED
                     EALIKEIKEKYNALSAEDQKKVENFAVLVDAQVTLDSLKFLAELDINYDNATLALLNQ
                     LKGKINNLSNDVASKIQNEINAAEDKINKYIKNLEFEAAVVALNGEANKEALAQLDLI
                     YGTITEEFKAKLNADIKATYLTLSAQAKEQGGDPEDPVDPEDPEDPDNPEDHNQNKEQ
                     LAIERYLTTVFPDKVSMGFVLPEVHSGVRFTYTSSDNIYFDPEFMFFMPDDDYHDLTL
                     TVHYTLSGVEYTTDIDVTLVPNKYSEAYDFIYNQFRPPIGASYDYINFEDRYNPTVTY
                     QIRSLNPEIMDNQLKLVEKPNVEQYITLMLIIQYPDEEPVEMEMILPVMAKTFLEKAR
                     AMEEVYLRYLEQFLDNGVLSQDLILPTKDEDFGVDLTWSSDTPAFLSDDGVYTGPVGN
                     DGAQVVLSMNVHSKDQSAVHTIAYRLYLKGADTPEGWDAVEYFLNQINLQHIKNQSFF
                     TYGATT"
     gene            complement(1538..2416)
                     /locus_tag="PLQ22_02985"
     CDS             complement(1538..2416)
                     /locus_tag="PLQ22_02985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH58958.1"
                     /translation="MDIFEKVEIFLNKIHKDEIVNYPFYLYGFEEEYKKVKSYNNGYL
                     PFYINKDLKITQNFLPKENTLNRPGLLREKTKYVVIHDAGLASPLMTAKGLDQYLHTT
                     TRQASWHYSVDDKEVYQELPLEEVAWHAGDGRTSYPENWLNDKGFHLGGGNYTGIGIE
                     TCVYKGVNFNQAMRNVAKLTAWLLIKYDLSVNDVKQHNDFSGKNCPETIRTANRWDEF
                     IELVNLEYYKNTKLADVELEFVSLDPQFLDNEGNVLKHPLRDKIVSYEVKANYQGETR
                     TFKYQTKIKKLLEVEL"
     gene            complement(2514..4946)
                     /locus_tag="PLQ22_02990"
     CDS             complement(2514..4946)
                     /locus_tag="PLQ22_02990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH58959.1"
                     /translation="MKKKLGIFLIFLFGILILSGCTGCEKKPKPEEDFEKVIQTIQNL
                     PNTEDLMLVDKENVEAAFSQYNALNEAAKAKVSNYQKLNAARAKIQELEAIASADMID
                     SKILELTEPVTLADEALYVEIQELITAASEAARGRITNLVKFNSMFSQYETLKNDRNA
                     KQTILDNINEEIGQLADPTTLDDEKKYNSISEKIGELSEEDKKGIASLDRFNTKHKEF
                     LVLKEIDNINNKIALLQVPVTLADEKLYLELRTAIDNASAEVSTKIIGKAGFEEKYLD
                     YLGLKELENKQAARIVSDLIANLPDEVNKSDKAAIENARIKYEQLTSAQKEFVTNLAR
                     LIQKEEELALIDELENISAADQAAIAFANISKHYDDNYVIEENQNFFQRTPAYSKLTF
                     TWNTSDISVLSPTGELIGRPVFDSEIIITVTASSRRESFEESISFGVFVLGMDSKSDK
                     WQMIEKFLNYNNRLSIPNRKYKYHESISQTYHQSYGYLPFFTNYKLPIYDNFLPEGKK
                     TNGPASSIEWVVVHDTGSYGPSDTATAIANYIQSDAAVSWNYTVGETTISGVRQTVIF
                     HHMAEGMTTWQAGDGGNLFSLLDTGVAHKGHRNPIVTIGSDRYFYLNGQKTTLMIPSN
                     AIADNRVINENGLLVELGEDGNYKMADYWWCTQFYNPLGSRGYICNKGGNRNSVSMET
                     CAFDGANYTLTMRYMAALCAEILIRHDLPVERVSQHNRFSGKDCPHAIRAQGYWYDFI
                     EQVRIEWFGRKYLDDVSFVYEASGNYFDPKTGVVLNHPGPSTVVNYKVKATYQGVTKE
                     FKFTTTLEAVAN"
BASE COUNT         1653 a          948 c          674 g         1967 t
ORIGIN      
        1 gtagttgctc catatgtaaa gaatgattgg tttttaatat gttgtaaatt aatttgattt
       61 aagaaatatt caacagcatc ccaaccttca ggagtatctg ctccttttaa atatagacga
      121 taagcaattg tatgaactgc agattgatct ttgctatgaa cattcatcga taaaacaact
      181 tgtgcaccat cattacccac aggaccagta tatactccat catcagataa aaatgctggt
      241 gtatcacttg accaagttaa atctacacca aaatcttcat ctttggtagg taaaattaaa
      301 tcttgactca agactccatt atctaagaat tgttctagat atcttaaata tacttcttcc
      361 attgctcttg ctttttctaa aaatgttttt gccataactg gtaagatcat ctccatttct
      421 actggttctt catctggata ttgaatgatt aacattagtg taatatattg ttcaacattt
      481 ggtttttcaa ctaattttaa ttgattatcc ataatctctg gatttaaaga tctaatttga
      541 tatgtaaccg ttggattata tctatcttca aaattaatat aatcataact agcaccaatt
      601 ggtggtctaa actgattata gataaagtca tatgcctcag aatatttatt aggtactaaa
      661 gtaacatcaa tatcggtagt atattcaact cctgataaag tataatgaac agttagtgtt
      721 aaatcatgat aatcatcatc tggcataaaa aacataaatt caggatcaaa atagatgttg
      781 tcagatgaag tataagtaaa tctaacacct gaatgaactt ctggaagtac aaatcccatt
      841 gaaactttat ctggaaaaac agtagttaga tatctttcta tagctaattg ttccttattt
      901 tgattatggt cttctgggtt atctggatct tccggatctt ctgggtccac aggatcttct
      961 ggatctccac cttgctcctt agcttgcgca gataacgtaa ggtatgttgc tttaatgtct
     1021 gcattcaatt tggctttaaa ttcttcagtt attgtcccat atataagatc taattgagct
     1081 aatgcttctt tatttgcttc accatttaaa gcaactactg cagcttcaaa ctctaaattc
     1141 ttaatatatt tattaatttt atcttctgct gcgttaattt cattttgaat tttactagct
     1201 acatcattac ttaaattgtt tatctttcct tttaactgat ttaaaagagc taaagtagca
     1261 ttgtcatagt tgatgtctaa ttcagctaag aattttaaac tatcaagtgt aacttgtgca
     1321 tctaccaaaa cagcaaaatt ttcaactttc ttttgatcct cagcacttaa agcattatat
     1381 ttttctttga tctctttaat taatgcttca tcttctagag taacttcacc aatattgtca
     1441 attaagctct ctacagcctt aaatgcttca tctttatcct tgcaaccaac taaggtcacc
     1501 aaaaggaaaa gaactaaaat aaatttcaat acttttttca taattccacc tctaataatt
     1561 tcttgatttt tgtttgatat ttaaatgttc ttgtttcacc ttgatagttt gcttttactt
     1621 cataactaac tatcttgtct cttagcggat gttttaatac atttccttca ttgtctagaa
     1681 actgtggatc taaactaaca aattctaatt ctacatctgc taacttagtg tttttataat
     1741 attctaaatt aactaactct ataaactcat cccaacgatt agctgtccta attgtttctg
     1801 gacaattctt gcctgaaaag tcgttgtgtt gtttaacatc gtttactgat aagtcatatt
     1861 taattaataa ccaagccgtt aatttagcaa catttctcat tgcttgattg aaattaacac
     1921 ctttataaac acaagtttca attcctattc ctgtatagtt cccaccacct aaatgaaatc
     1981 ccttatcgtt taaccaattt tctggataag aagttctacc atcacctgcg tgccaagcaa
     2041 cttcttctaa tggcagttct tgatatactt ctttgtcgtc aacactataa tgccaagaag
     2101 cttgtcttgt agtggtgtgt aaatattgat ctaaaccttt agctgtcatc aaaggtgaag
     2161 ctaaaccagc atcgtgaata actacatatt tagttttttc tcttaacaaa cctggtctgt
     2221 ttaaagtatt ttcttttgga agaaagtttt gtgttatttt taaatcctta ttaatataaa
     2281 aaggtaaata tccattatta tatgatttta cttttttata ttcttcttca aaaccataaa
     2341 gataaaatgg ataattaaca atttcatctt tatgaatttt atttaaaaag atttctactt
     2401 tttcaaaaat atccattctc tacctcttaa tatatattat atcataaact tacaaacata
     2461 acaaacaaaa agagcccacg aagggctctt tttgttatca tatattttta atattaattt
     2521 gctacagctt ctaaagttgt agtaaattta aattctttag taactccttg ataagttgct
     2581 tttactttgt aattaacaac tgtagaaggt cctggatgat ttaaaaccac tcctgtttta
     2641 ggatcaaagt aattaccact tgcttcatat acaaagctta catcatctaa atacttacga
     2701 ccaaaccact caatcctaac ttgttctata aagtcatacc aataaccttg tgccctaatt
     2761 gcgtgaggac aatcttttcc agaaaaacga ttatgttgag aaactctctc tactggtaaa
     2821 tcatgtctaa ttaaaatctc agcacataaa gcggccatat atctcattgt taacgtatag
     2881 ttagcaccat caaaagcgca agtttccata ctaacagagt ttctatttcc acccttattg
     2941 caaatatagc ctctagatcc tagtgggtta taaaattgtg tacaccacca gtaatcagcc
     3001 attttataat ttccatcttc accaagttct actaaaagtc cgttctcatt aataactcta
     3061 ttatctgcga ttgcattgct tggaatcatt aaagttgttt tttgtccatt taaatagaaa
     3121 tatctatcag aaccaatagt aactatagga tttctatgtc ctttgtgggc aacacctgta
     3181 tcaagtaaac taaataagtt tccaccgtca cctgcttgcc aagtcgtcat accttctgcc
     3241 atatgatgga aaattactgt ttggcgtact ccacttattg ttgtttcacc tactgtatag
     3301 ttccaagaaa cagccgcatc gctttgaata tagttagcaa ttgcggttgc agtatcgctt
     3361 ggtccataac ttccagtatc atgaacaact acccattcaa ttgaagatgc aggaccattt
     3421 gtctttttac cttcaggtaa gaaattatca taaattggta atttatagtt agtaaagaat
     3481 ggtaaatatc cataactttg atgatatgtt tgactaatac tttcatgata tttatatttt
     3541 ctatttggaa tagataaacg gttattgtag tttaagaact tttcaatcat ttgccattta
     3601 tcggattttg aatccattcc taaaacaaat actccgaaac taatagactc ctcaaaactt
     3661 tctcttcttg aagaagctgt cacagtaata ataatttcag aatcaaatac tggtcttccg
     3721 ataagctccc cagttgggct taaaacagag atatcgctag tattccaagt aaatgttagt
     3781 ttactatagg ctggtgttct ttggaaaaag ttttggtttt cttcaattac ataattatca
     3841 tcataatgct tagaaatgtt ggcaaatgct atcgctgctt gatctgcagc tgatatattt
     3901 tcaagttcat caattaaagc taattcttct tctttttgaa ttaaacgagc taaattagta
     3961 acaaactctt tttgagctga agtcaattgt tcatatttaa tgcgagcatt ttcaattgct
     4021 gctttatcgc ttttatttac ttcatctggt aaattagcaa ttaaatcact aactattcta
     4081 gcagcttgtt tgttttctaa ttcttttaat cctaagtaat ctaaatattt ttcttcaaat
     4141 cctgcttttc caataatttt tgttgaaact tcagcactag cattatcgat agctgttctt
     4201 agttctaaat ataatttctc atcagctaaa gtaactggta cttgcaataa agctatctta
     4261 ttattaatat tatcaatttc ttttaatacc aaaaattctt tatgtttggt attaaaccta
     4321 tctaatgatg caattccttt tttgtcttct tcacttagct ctcctatttt ctctgaaatt
     4381 gagttatatt ttttctcatc atctaaagta gtaggatcag ctaattgacc aatttcttca
     4441 ttaatattat ctaaaattgt ttgcttagca ttacgatcat tttttaacgt ttcatattga
     4501 ctaaacatac tattaaattt tactaaattt gtaatacgcc ctcttgcagc ttcacttgct
     4561 gctgttatta attcttgtat ctcaacatag agagcctcat cagctaaagt tacaggttct
     4621 gtcaactcta atattttcga gtctatcata tctgcactag ctatggcttc caattcttga
     4681 atctttgctc tcgcagcatt aagtttttgg taattactta ctttagcttt agctgcttca
     4741 tttaaagcat tatactgaga aaaagccgcc tcaacatttt ctttgtctac cagcattaaa
     4801 tcctcagtat taggtagatt ctgaattgtt tgaataacct tttcgaaatc ttcttcaggc
     4861 tttggtttct tttcacaacc tgtacagcca gaaagaatta gtattccaaa aagaaagatc
     4921 aagaaaatac ctaatttttt cttcattttg tctcctccta taactcttat aacaaaaata
     4981 ttatactata tttttattgg attgtcaata tccagaatga cctctaaagt ggtagatatt
     5041 acttaaaaaa cgactcttaa ttacacctta tatagccata taataaaaaa tgaagacctt
     5101 aatcttcatt aaaatttcat aattatagat aaagcaaggg acaatccctt gctcatatta
     5161 tagttagtgt tatcaaaact agctaacaaa tggtggtcga ggacggaatt gaaccgccga
     5221 cacaaggatt ttcagtcctt tg
//
LOCUS       DAOSGS010000056         5215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14120_length_5215_cov_3.682558, whole genome shotgun sequence.
ACCESSION   DAOSGS010000056 DAOSGS010000000
VERSION     DAOSGS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14120_length_5215_cov_3.682558"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1034
                     /locus_tag="PLQ22_02995"
     CDS             186..1034
                     /locus_tag="PLQ22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH58960.1"
                     /translation="MRKIKIIIDSTSDLGKEVYEKHDIEIVPIYVILGDNTYKDYYEM
                     DSTKMFAYTEETGIYPKTAAASPLWIYKVFKKYYDEGYDIIYTGISSKLSSTYSTALT
                     AAREIDPNEERIFLFDSENLSTGVGLLVLKIIKYRDQGLSAKEIRDKVKELVPNVKTQ
                     FTLNTLEYMQKGGRCSAVVRYAAAAFRFKPVIKVNDGLLEVARKPIGFKRSINSMLLD
                     AENAFPTADQDAIFVTYSSYGDWVDYLVAELKKIGYKNVYTTLAGATICTHCGPQCVG
                     ILYIDN"
     gene            1144..3198
                     /gene="fusA"
                     /locus_tag="PLQ22_03000"
     CDS             1144..3198
                     /gene="fusA"
                     /locus_tag="PLQ22_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH58961.1"
                     /translation="MKQCKSDHIQNIAILGHLNSGKTSLTEAMLFEAKAISKKGSVDK
                     KNTVSDYLLEEQTRQTSLISSLLPLEWKDYKINFLDTPGSEEFIGDLENVLSVVEGAI
                     LLVDASSGVEVGTEACWSELRKRNIPTLIILTKMDKENVKFEDVLAEIKGQLSDNAIP
                     FSLPLGKSANFDGYINVITKKARIFKDGKYHDDVAYDDKKDRVEELYEQLLEKVAESS
                     EELLEKYFSGEELTPDDVRKGLLSGTKKGDVFPVGVISVENNVGISTLLDMVVKYLPS
                     AGGREYQAFDQDGKEIVRKSLESEPFSAQIFKTTVDPFAGTISFMKVKSGVLKENSEV
                     KVANLNEDIKISNIFSMRGNVQISVSEAPAGDIVCVAKVDELRNSMTLCDKRVFTKYP
                     AVEHPTPILYMAIQPKNRQDEDKLSSSLNKLKLEDETFETVRNPETAQLLIGGQGTTH
                     IGYILERMKNTYKVEVDTYDQKVVYRETIRAKGEAQGRHKKQSGGAGQFGDVFIRFEP
                     TEEEFVFAEEIFGGAVPKNYFPAVEKGLRETFEKGPLAGFPVVGVKCTLYDGSYHPVD
                     SNEISFRLAANLAWRNAVPNLRPTILEPIYRVDVTVKDDYVGDIMGDITKRRGRVLGL
                     EQKEGFQVIMAEVPEAEITKYAIDLKAMTQGSGRFARTFLRYEAVPENLIDGIVKEYK
                     AQ"
     gene            3245..4060
                     /locus_tag="PLQ22_03005"
     CDS             3245..4060
                     /locus_tag="PLQ22_03005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH58962.1"
                     /translation="MKLEYIVDEDEIIVKDYLEKQNISKRLAKKIKLYGKTYVNKIEV
                     FNYFLLSKGDHLVVEFEDQDTSIYPPVNEELDIIYEDEYFMIVDKPENLASLPTKSHF
                     QDSLASRIKYYYQTKNINTKVHLVNRLDYATSGLVIVAKDTYIKSRFKNLNIERKYLA
                     VLDNDLEDEGIINLPISKKENSLLREVNEKGKEAITIYKVIDKTRKLYEFTLVTGRTH
                     QIRIHTSFYNAPIKGDKLYGKESDRLYLTCYYLKFKHPILDEKMEFVSYPKWH"
     gene            4133..>5215
                     /locus_tag="PLQ22_03010"
     CDS             4133..>5215
                     /locus_tag="PLQ22_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH58963.1"
                     /translation="MKKFGKILIAFILVLSLGFLVGCSCEDAKLEGIEVTPASLTLDA
                     DEIQQLEVKPVPEGVALPAASFTSSDSTVASVGKDGKVMGIKAGTAEITVKVGEFTKK
                     VTVTVNPILASDLTVPAALEVKVGATATINYTIAPANVTNKVATFTTSDASIATVDAE
                     GVVTGVAGGTATITVKADAIEKGVVVTVVPAVALESITLKQEDQNLNLGQTLQLSCTL
                     VPEDTGLAVSYASSDEAVATVSSSGLVTAVGVGSATITASAGGKSDTIEITVVDDEDL
                     TLDNLQAKLNNAIAAYNNSLNGSVKLSVVTPETTVVTEFAYAYTEDSISELMYKQVGA
                     TSIEVYVKNGKIYTNENGSKSSEALTA"
BASE COUNT         1972 a          633 c          989 g         1621 t
ORIGIN      
        1 attagcacgc attgtggccc agaaacaatt ggtatagtat acatatataa ataaaagttg
       61 tgctttaaac acaacttttt attcttagat taaatatata agtaaaagtt tttaaacaaa
      121 agactttaat atttttgtga tttttgttat aatacaatat gtagcgaaag aaaaggggga
      181 gaaaattgag aaaaataaaa ataatcatcg atagtactag tgatttagga aaagaagttt
      241 atgaaaaaca tgatatagaa attgttccta tctatgtaat tttaggggac aacacatata
      301 aagattatta tgaaatggat agtacaaaaa tgtttgctta tactgaagag acaggtattt
      361 atccaaaaac ggcagcagca tcacctttat ggatttataa agtattcaag aaatattacg
      421 atgaaggata tgatattatt tatacgggga ttagtagtaa attatcgagc acctattcta
      481 ctgctttaac agctgcaaga gaaattgatc ctaatgaaga aagaattttc ttatttgata
      541 gcgaaaattt atcaactgga gttggacttt tagttttaaa gattattaag tatcgtgacc
      601 aaggcttaag tgctaaggaa ataagagaca aagttaaaga attagttcct aatgtcaaga
      661 cacagttcac tttaaatact ttagagtata tgcaaaaagg gggaagatgt tctgctgttg
      721 taagatatgc agctgctgcc tttagattta aacctgttat taaagttaat gatggtcttt
      781 tagaggttgc gagaaaacca attggtttta aaagatcaat taactcaatg ttattagatg
      841 cggagaatgc ttttcctaca gctgaccaag atgctatctt tgtaacttac tcttcatatg
      901 gtgattgggt agattactta gttgcagagc ttaagaaaat tggttataag aatgtttata
      961 ctacacttgc aggggcaaca atttgtactc actgtggtcc tcaatgtgtt ggtattttat
     1021 atattgataa ttaatagttg tgtttaatac acaactattt ttattttttt tcttaaaaca
     1081 gtggaaatta aaagcgtttt atagtataat attaaaagtg aaataactat aaggaggaaa
     1141 tatatgaagc aatgcaaaag tgatcacatt caaaatattg caatcttagg acacttaaac
     1201 tcaggaaaaa cttcattaac tgaggctatg ctgtttgaag caaaagctat ttctaaaaaa
     1261 ggtagtgttg ataagaaaaa tacagtaagt gattatcttt tagaagaaca aacgcgacaa
     1321 acatctttaa tctcaagttt gttgccatta gagtggaaag actataaaat taactttctt
     1381 gacactccag gttctgaaga atttatcgga gatttagaaa atgtattatc ggttgtagag
     1441 ggagctattt tactagtaga tgcatcaagt ggtgttgaag taggaactga agcatgttgg
     1501 agtgagttaa gaaagagaaa tatcccaaca ttaattatcc ttacaaagat ggacaaagaa
     1561 aatgttaagt ttgaagatgt actagcagaa atcaaaggac aactttctga taatgcaatt
     1621 ccatttagtt tacctttagg taaaagtgct aactttgatg gttacattaa tgtaattaca
     1681 aaaaaagcaa gaatttttaa agatggaaaa taccacgatg atgtagctta tgatgataaa
     1741 aaagatcgtg tagaagaatt atatgaacaa cttttagaga aagttgcgga aagtagtgaa
     1801 gagttattag agaaatattt ctctggtgaa gaattaactc cagatgatgt tcgtaaagga
     1861 ttactttctg gaactaaaaa gggagatgtt ttcccagtag gtgttattag tgttgaaaac
     1921 aatgtaggta ttagtacatt acttgatatg gttgttaagt atttaccatc tgcaggggga
     1981 agagaatatc aagcttttga ccaagatgga aaagaaatag taagaaaatc actagaatca
     2041 gaaccattca gtgcacaaat tttcaaaacg acagtagacc catttgcagg tacgatttca
     2101 tttatgaaag ttaagagtgg cgttttaaaa gaaaactctg aagttaaagt tgctaaccta
     2161 aatgaagata ttaaaatttc taacatcttt tcaatgagag gtaacgttca aatttctgta
     2221 agtgaagctc cagctgggga tatcgtatgt gttgcaaaag tagatgagtt aagaaactca
     2281 atgactttat gtgataaaag agtatttact aaatatccag cagttgaaca tcctacacca
     2341 attctatata tggcaattca acctaagaat cgtcaagatg aagataaatt atcatcatca
     2401 ttaaataaat taaagttaga agatgaaaca tttgaaactg taagaaatcc tgaaactgca
     2461 caattgttaa ttggtggtca aggaactact catattggct atattttaga gcgtatgaaa
     2521 aacacttaca aagtagaagt agatacatat gaccaaaaag tagtttatcg tgagacaatt
     2581 agagctaaag gtgaagctca aggtagacat aaaaaacaat caggtggagc agggcaattt
     2641 ggtgatgtat ttattcgttt tgaaccaact gaagaagaat ttgtgttcgc tgaagaaatc
     2701 tttggtggag cagttccaaa gaactacttc ccagctgtag aaaaaggttt aagagaaaca
     2761 tttgaaaaag gtcctttagc aggattccca gttgtaggtg ttaaatgtac tttatatgat
     2821 ggttcttatc acccggttga ctcaaatgag atttcgttta gacttgctgc aaaccttgct
     2881 tggagaaatg ctgttcctaa tttaagaccg actattttag agcctattta tcgtgtagac
     2941 gtaacagtta aagatgatta tgtaggtgat attatgggag atattactaa gagaagagga
     3001 agagtattag gtctagaaca aaaagaaggt ttccaagtga ttatggctga agtaccagaa
     3061 gcagaaatta caaaatatgc aattgactta aaagctatga ctcaaggtag tggacgtttt
     3121 gctcgtacat tcttaagata cgaagcagta cctgaaaact taattgatgg tatcgtaaaa
     3181 gaatataaag cacaataaga aaaaggctat ctattaagat agccttattt aataggagtt
     3241 atttatgaaa ttagaatata tcgtagatga agatgagata attgtaaagg actatttaga
     3301 aaagcaaaac atttctaaaa gattagctaa gaaaataaaa ctatatggta aaacgtatgt
     3361 caataaaatt gaagttttta attacttttt attatcaaaa ggagaccatt tagttgtaga
     3421 gtttgaagat caagatacta gtatttatcc tccagtaaac gaagagttag atataattta
     3481 tgaagatgag tattttatga tagttgataa accagaaaac ttagccagtt tacctacgaa
     3541 atctcatttt caagatagtc ttgcaagtag gattaaatat tattatcaaa caaaaaatat
     3601 taatacgaaa gttcatttag tgaatagact agattatgca acatcgggat tagtgattgt
     3661 agctaaagat acttatataa aatcaagatt taaaaatcta aatattgaaa gaaaatattt
     3721 agctgtttta gataatgatt tagaagatga aggcattatt aatcttccaa tttctaaaaa
     3781 agaaaatagt cttttaagag aagtaaacga aaaaggaaaa gaagctatta ctatatataa
     3841 ggtaatagat aaaacgagaa aactttatga atttacttta gttacaggaa ggactcacca
     3901 aattagaatt catactagtt tttataatgc tcctattaaa ggagataaat tatatggtaa
     3961 agaaagtgat cgtttatatc taacatgcta ttatttaaaa ttcaagcatc caattttaga
     4021 tgaaaaaatg gaatttgttt cttatccaaa atggcattaa ttattttaaa aaagaattga
     4081 ttaaaaaatt agattgtgtt ataattatat tataaaagaa gcggaggaaa aaatgaaaaa
     4141 atttggaaaa atattgatag cttttatttt ggtgcttagt ttaggttttc tagttggttg
     4201 tagttgcgaa gatgcaaaac tagaaggtat cgaagtaaca ccagcatcat taacattaga
     4261 tgcagatgaa attcaacaat tagaagttaa acctgttcct gaaggagtag cattaccagc
     4321 tgcaagtttt acgtcttcag atagtactgt tgcaagtgta ggaaaagatg gtaaagttat
     4381 gggaatcaaa gcaggtacag cagaaattac agtaaaagta ggcgaattta caaagaaggt
     4441 tactgttaca gttaatccaa tcttagcatc tgatcttaca gtaccagctg cattagaggt
     4501 aaaagtagga gctacagcaa ctattaatta tacaatcgct ccagcaaacg ttacaaataa
     4561 agttgctaca ttcacaacaa gtgatgcaag cattgcaact gtagatgctg aaggtgtagt
     4621 aacaggtgtt gccggaggta cagctacaat tacagtaaaa gctgatgcta ttgaaaaagg
     4681 tgtagtagtt acagttgttc cagctgttgc attagaatca ataactttaa aacaagaaga
     4741 tcaaaattta aatttaggtc aaacattaca attatcttgc acattagtac ctgaagatac
     4801 aggattagct gttagttatg cttcaagcga tgaagctgtt gctactgtat catctagtgg
     4861 tttagtaact gctgttggag taggttcagc gactattact gcaagtgcag gtggaaaatc
     4921 agatacaatt gaaattactg ttgttgatga tgaagacttg actttagata acttacaagc
     4981 taagttaaat aatgcaattg cagcttataa taattcatta aatggttctg ttaaattgtc
     5041 tgtagttact cctgagacaa ctgtagttac tgagtttgct tatgcttata cagaagattc
     5101 aattagtgaa ttaatgtaca aacaagtagg tgctacttca attgaagttt atgtaaagaa
     5161 tggcaaaatc tacactaacg aaaacggaag taagagtagt gaagctttaa ctgct
//
LOCUS       DAOSGS010000057         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14170_length_5202_cov_3.231786, whole genome shotgun sequence.
ACCESSION   DAOSGS010000057 DAOSGS010000000
VERSION     DAOSGS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14170_length_5202_cov_3.231786"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..908)
                     /locus_tag="PLQ22_03015"
     CDS             complement(132..908)
                     /locus_tag="PLQ22_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HOH58964.1"
                     /translation="MKVNNLKINFELVNGLILIKGRINDEEGYFLVDTGASKTVIDSR
                     HFPNLEKIETEEKAIIFNQGIKDVIAYQTKVDIIKIESLEIKDYLVRILDLSYFSNFF
                     EDHKIIGVLGLDVLTNYRVLIDYEREELIFNPKENFENQEVIDLLVEPELLCAVKVTI
                     NDYSFRVIIDTGANACLISSKIKEEVKLNYEINEPFLLEKLTIGLKEYNDLVTVISNT
                     DIFEYEDVYGLIGSEVLGKQRIIIDFIEEKLYLENKKTEN"
     gene            complement(883..1272)
                     /locus_tag="PLQ22_03020"
     CDS             complement(883..1272)
                     /locus_tag="PLQ22_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58965.1"
                     /translation="MKLQTTKTLAIISIALYGLLVVMSILFALFVIDKTFILATISPE
                     ELEATPFPYLRYLLYGTVVFSLLIALLFMAYQVLYTLVFVGTIGFKKKTTMILLIIGY
                     FVWVVALVGLIMTLVHKDKDESKQLEN"
     gene            complement(1253..1660)
                     /locus_tag="PLQ22_03025"
     CDS             complement(1253..1660)
                     /locus_tag="PLQ22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58966.1"
                     /translation="MDASKTKKMAITSLIMQGVAFVLTIIFVALVFKDLIAFIEQHGE
                     GTTPEFTDAIRQLYSPSTRIVLLLKSLFGVADLILIIMIVVETSKLKSKTPMIFLIIG
                     LAVDVLKIVGLIMTLIQCNKQSKAGDASETANN"
     gene            complement(1873..2289)
                     /locus_tag="PLQ22_03030"
     CDS             complement(1873..2289)
                     /locus_tag="PLQ22_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH58967.1"
                     /translation="MCKILKAYCIAKKAHKGQKDKGGKPYIKHPVYVAKRVKGRDAKI
                     VALLHDTIEDTSVTYDYIKSVFGDRIAESVKVLTKDKTISYEEYIALIKQNDLARRVK
                     IEDLKHNMDLKRLKVVTEEDLKRVEKYKHSLEILSD"
     gene            complement(2364..3014)
                     /locus_tag="PLQ22_03035"
     CDS             complement(2364..3014)
                     /locus_tag="PLQ22_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58968.1"
                     /translation="MNINVFEDQHLKYFLYLEKEVMSTFEYSTLDIMNKNNFSFKYIN
                     LLQAICSEFEVVAKAYCELLNEPDADSILKYGKVIIGEHPEITTKNVRCYENSNLIYV
                     PFQDWVIPTAGNKSEKPPKWWTIYNKIKHNRLALNNDGEYKGIENYKLANQDNVMNAL
                     AGLYLLEMYFFKDLTLKSPNVASDIYIPTGQYVSNLFDLPNWSSTISIGPLIINNI"
     gene            complement(3057..4217)
                     /locus_tag="PLQ22_03040"
     CDS             complement(3057..4217)
                     /locus_tag="PLQ22_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58969.1"
                     /translation="MYNQIVIDELINFIHNNPNLSKEKLTEAVVKKFQLTKDRSVYYN
                     KYYAIRFSKSSSDNFSNTVLGLSTLQKYDHLPFIICLNTPNENKLMLANTSFLKKISH
                     SSHKLRRDNIRGSFNGTDIVRRFGTIENNENNFEELFSFHQEIPFEENLDRLVESTNN
                     IIGRKLRYDSNPKGDANILNSVDRAEKFIASKYYDELNADLFTKVKKVRNEILIATLI
                     DNVNIRGRVIEYLITHGNSDNLSANIIKALQKKEPLPEIKTDNDLGDYNKNFDLYDSK
                     TDIKTKILYLKSAPKAFNIDKLLTFLSKDKSIYLIYFVGFESNNEIKTFLCSVFNTKL
                     LEGTGVQFHWAGRNSRGVAQFNGAIIDDILNEKLNIINKNKAREWLQYLISI"
     gene            complement(4242..>5202)
                     /locus_tag="PLQ22_03045"
     CDS             complement(4242..>5202)
                     /locus_tag="PLQ22_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020064149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOH58970.1"
                     /translation="TNKKQVKTKKTTRNKTIDFEITQDNKFVQEVIQITEKQSLSNVI
                     NKTINGDTFEVLKLLPEKIIDLLIVDPPYNLSKNYHGNIFDKQKELEYENYTKKWIDA
                     VIHTLKDDATIYVCCDWESSLIIGKVLKEYFKVKNRITWQREKGRGAKTNWKNAMEDV
                     WFATNSNNYTFNVNDVKVRKKVIAPYRINGQPKDWNETIEGNFRDSYPSNFWDDITIP
                     FWSMQENTAHPTQKPEKLIAKLILASSNKGDVVLDPFLGSGTTSVVAKKLGRKYIGIE
                     YNMQYCAWAEYRLEKANVDNTIQGYVDGVFWERNTLAAQQKNK"
BASE COUNT         1764 a          852 c          541 g         2045 t
ORIGIN      
        1 aaggagaatc aatgcgatat acagatggta aatactacat cgctactaga gatgctgttc
       61 caggtagaac acctgaaaac tctccagacc actggagaga agtttcttgg taatataaaa
      121 aagactgaaa tttaattttc agtcttttta ttttctaaat ataacttttc ttcaataaaa
      181 tcaataatga ttctttgttt tcctaatact tcagaaccaa ttaaaccata aacatcttca
      241 tactcaaaga tatcagtatt actaataaca gtaactaaat cattatattc tttcaaacca
      301 atagtaagtt tttctaaaag aaaaggttca tttatttcat aatttaattt tacttcttct
      361 tttatcttac tacttattaa gcaagcatta gcaccagtat caataattac tctaaaagaa
      421 taatcattta ttgtaacttt aactgcacaa agtagttctg gttctactag taaatcaatt
      481 acttcttgat tttcaaagtt ttcttttgga ttgaaaatta actcttccct ttcatagtca
      541 attagaactc tatagtttgt tagaacatct agacctaaaa caccaataat tttatgatct
      601 tcaaaaaagt tcgaaaaata acttaaatct aatatcctta caagataatc tttaatctct
      661 aaactttcta ttttaattat atctacttta gtttgataag ctattacatc tttaatccct
      721 tgattaaaga taatagcttt ttcttctgtt tctatctttt ctaaattagg aaaatgccta
      781 ctgtcaataa ctgtttttga tgctccagta tctacaagaa aataaccttc ttcatcatta
      841 atcctcccct ttattaggat taagccatta acaagttcaa aattaatttt caagttgttt
      901 actttcatct ttatctttat gcaccaaagt cataattaaa ccaactagtg caactaccca
      961 tacaaaatac ccaataatca ataaaatcat cgttgttttc tttttaaacc ctattgtccc
     1021 tacaaatacc aaagtataaa gaacttggta tgccataaac aacaaagcta taagtaaaga
     1081 aaaaacaaca gtcccatata ataaatacct taaataagga aaaggtgttg cttctaattc
     1141 ttcagggctt attgtggcaa gtataaaagt tttatcaatt acgaataatg caaacaaaat
     1201 actcataact accaataacc cataaagagc tatactaatt atcgctaatg ttttagttgt
     1261 ttgcagtttc acttgcatct cctgctttag attgtttgtt gcattgaatt agtgtcataa
     1321 ttaaaccaac aatctttaat acatctactg ctaatccaat tataagaaaa atcattggcg
     1381 tttttgactt taacttagat gtttcaacta caatcataat tattagaatt aaatcagcta
     1441 ctccaaataa actcttcaat aataaaacaa tacgtgtgct tggtgaatac aattgcctaa
     1501 tagcatctgt aaattcaggt gtagtacctt caccatgttg ttcaataaat gcaatcagat
     1561 ctttaaaaac taatgcaaca aatataatag ttaaaacaaa agctactcct tgcattatta
     1621 aagatgtaat ggccattttt tttgttttac ttgcatccat atattctacc tcctatcatt
     1681 tatattatac taaaaattct taattcaatc ttaattaatg cttggaggga ttgtaaaaaa
     1741 tttgcatata aaaattatat atgtaaattt tcacataaat atttattgtt atattttaat
     1801 ttataacaaa aaactgtttt aactactctt ctagattaaa acagttttac atctttaact
     1861 attcaaaaaa tgttaatcac ttaaaatttc taatgaatgt ttatattttt caactctctt
     1921 taaatcttct tctgtaacta cctttaatct cttcaaatcc atattatgct ttaaatcttc
     1981 aatctttact cttcttgcaa gatcattttg ttttattaaa gcaatatatt cttcataaga
     2041 aatagtctta tctttagtta acacctttac agattctgca atcctatcac caaatacgga
     2101 ttttatataa tcataagtaa cactagtatc ttcaattgtg tcatgtaaaa gagctactat
     2161 cttagcatct cttcctttta ctcttttagc aacataaaca ggatgtttaa tataaggttt
     2221 acctccctta tctttttgtc ctttatgagc ttttttagct atacaatatg cttttaaaat
     2281 tttacacata aattagtaac tccaatatta tattccttta gtgaaaaaat tgtatcatag
     2341 ttaatattaa attaaatttc atcctagata ttattaatta ttagtggtcc aattgaaata
     2401 gttgatgacc aattcggtaa atcaaataaa ttagacacat attgtcctgt tggaatataa
     2461 atatcagacg ctacattagg agattttaaa gtcaagtctt taaaaaaata catttctaat
     2521 aagtataatc cagccaaagc attcataaca ttgtcttgat tagcaagttt ataattttca
     2581 atccccttat attctccatc attattcaag gcaagcctat tatgtttaat tttattataa
     2641 atagtccacc atttaggggg tttttcactc ttatttccag cggtcgggat tacccaatct
     2701 tgaaatggga catatataag gttactattt tcataacacc ttacattttt tgttgttatt
     2761 tctggatgtt cacctattat aacttttcca tattttaaaa tggaatcagc atcaggctca
     2821 tttaataatt cacaataagc ttttgcaact acctcaaatt cactacaaat tgcctgtaat
     2881 aaattaatat atttaaatga aaaattattt ttattcatta tatccaatgt tgaatattcg
     2941 aatgtactca taacttcttt ttctaaatat aaaaaatact ttaaatgttg gtcttcaaaa
     3001 acattaatat tcatatattc ctcctttcga ttgaaaaata cttaatatat gcataactaa
     3061 atagaaatta aatattgtaa ccattctctt gctttgtttt tattaataat atttaatttc
     3121 tcattcaaaa tatcatctat aatagctcca ttgaattgcg ccacacctcg tgaattcctt
     3181 ccagcccaat gaaattgaac acccgtcccc tcaagtagtt tagtattaaa aaccgagcaa
     3241 agaaatgtct taatttcatt gtttgattca aatccaacaa aataaatcaa atagatagat
     3301 ttgtctttgg ataaaaaagt taatagttta tctatattaa acgccttagg tgctgatttt
     3361 aaatacaaaa tttttgtttt aatatcggtt ttagaatcat acaaatcaaa atttttgtta
     3421 taatcgccta aatcattatc agttttaatt tctggtaatg gttctttttt ttgcaaagct
     3481 ttaataatgt tggcactaag attatcagaa tttccatgag taattaaata ttctatgact
     3541 cgtcctctta tatttacatt atcaattaaa gtagctatca atatttcatt cctaactttt
     3601 tttactttag taaataaatc cgcatttaat tcgtcataat attttgaagc tataaatttt
     3661 tcagctctat caactgaatt taatatatta gcatcacctt taggattact atcatatctt
     3721 aattttcttc caataatatt attagtactc tctacaagtc tgtctaaatt ttcttcaaaa
     3781 ggaatttctt gatgaaaaga aaacaattct tcaaaattat tttcattatt ctcaattgta
     3841 ccaaaacggc gtactatgtc ggtgccatta aaggatcctc taatattatc tcttcttaat
     3901 ttgtgagaag aatgactaat ttttttcaaa aaacttgtgt tagctagcat taatttgttt
     3961 tcatttggtg tattaagaca aattataaaa ggtaaatgat catacttttg tagagtagat
     4021 aagcctaata ctgtattact gaaattgtcg ctactagatt ttgaaaacct aatagcataa
     4081 tatttattat aatacacgct tctatctttt gttaattgaa atttttttac tactgcttca
     4141 gttaattttt ctttacttaa atttggatta ttatgaataa aattaattaa ctcatcaata
     4201 acaatttgat tatacatatt gtacctccat attaaaatat tttatttgtt tttttgctgt
     4261 gctgctaatg tatttctttc ccaaaatact ccgtccacat aaccttgtat tgtattatca
     4321 acattagcct tttcaagtct atattctgcc cacgcacaat attgcatatt atactcaata
     4381 cctatgtatt ttctacctaa ttttttcgca actactgagg ttgtaccaga acctaaaaag
     4441 ggatccaata caacatcacc tttattacta ctagctaaaa ttaatttagc tattaatttt
     4501 tccggttttt gcgtgggatg tgcggtgttt tcttgcatag accaaaatgg aattgtaata
     4561 tcgtcccaaa aattacttgg ataagaatct ctaaaattgc cttctatagt ttcgttccaa
     4621 tctttgggtt gcccatttat tctatatgga gctataacct tttttcgcac tttaacatcg
     4681 ttcacattaa atgtgtaatt atttgaatta gttgcaaacc aaacatcttc cattgcattt
     4741 ttccaatttg tttttgctcc tcttcctttt tctctttgcc atgttattct attcttaact
     4801 ttgaaatatt cttttaagac tttaccgata attaaactgc tttcccaatc acaacataca
     4861 tagattgtgg catcatcttt taacgtatga ataacagcat ctatccattt cttagtataa
     4921 ttttcgtatt ctaactcttt ttgtttatca aatatatttc catgataatt ttttgataaa
     4981 ttatagggag gatcaacaat taataaatca ataatttttt cgggcaataa ttttagcact
     5041 tcaaaagtat ctccattaat tgttttatta attacattag acaaggactg cttttctgtt
     5101 atttgtataa cttcttgaac aaatttgtta tcttgtgtaa tttcaaaatc tatagtttta
     5161 tttctagtag ttttttttgt ttttacttgt tttttattag tt
//
LOCUS       DAOSGS010000058         5124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14463_length_5124_cov_4.707832, whole genome shotgun sequence.
ACCESSION   DAOSGS010000058 DAOSGS010000000
VERSION     DAOSGS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14463_length_5124_cov_4.707832"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="gltX"
                     /locus_tag="PLQ22_03050"
     CDS             <1..1010
                     /gene="gltX"
                     /locus_tag="PLQ22_03050"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOH58971.1"
                     /translation="YVIRQNIPIEGESSYTDLVFGEITVPNKDMEDNILIKSDGMPTY
                     NFAHVVDDHLMHVTYVIRGNEYLSSTPKYNLLYDAFGWEKPQYIHLTQIMKDETRKLS
                     KRHGDANFDDFIRKGYLPHAIVNYIALLGWSPKGTQEKFTMQELIENFSVEGLSKSNS
                     IFDEKKMAWLNGEYIKELSFEEFYKYALPYFEKSSIKGRYDYEPFARLLQSRIEIFSE
                     IPEKVKFIDEYQRFPSEKYENQRFKIDQELAYNILKSYLEKAEKIDFSEEAWKELVKE
                     VADLYQIKQGAVYSTMRWAISGVDVTPGGFVEIAVILGKEKSIERLKQSLAWLKEDLG
                     K"
     gene            1024..2415
                     /locus_tag="PLQ22_03055"
     CDS             1024..2415
                     /locus_tag="PLQ22_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH58972.1"
                     /translation="MKLTKKDYKEILEACLETGADFAEIYLEDKIANNYAMINGKLDR
                     ANSSNLYGASIRILQGVEESFGYTSDLSKENLLNLARKLNTSFNAPKQKIKFTLKEEE
                     TPKIIFVERSASKTKLKEKIKLLARAEKGLRSFDSKEIIRTNAVLTDEEQYITVINTL
                     GVIKKDLRSHLRLSVSAVASDGKNMQDAFGSKGGNCGYELFDNFDIEKMGYETSETAV
                     KMLHADEMKSGKMTVVIDNGFGGVLLHEACVHSLEATSVAKGSSVFCNMLGKQIASPI
                     VTAIDDGTIPNAWGSLNIDDEGNETKRNVLIKDGILQSYLVDFRNNRTMKHGITGSGR
                     RQNFKYSPTSRMTNTFFAPGDSTFEEIIKNTESGFFAKSMGGGSVNPATGEFNFAVNE
                     GYLIENGKITKPVRGATLVGSGAEILANIDMIANNLEFSYGMCGSLSGQIPTIVGQPT
                     IRVQNITVGGKGN"
     gene            2417..3730
                     /locus_tag="PLQ22_03060"
     CDS             2417..3730
                     /locus_tag="PLQ22_03060"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH58973.1"
                     /translation="MDYKKILEQGKKLGFSDLEFQISSSTSLSIDVFKGKVDKNQVHS
                     DFSIVLSGLYKDQLASVSTTNKDFDPKKLLKKLKENAKALNKDEKEEIFAGSKEYPKS
                     IVHKVDFNNIDNKKKVDFLLDLEKKVYDFDKRITLVSNCRYFESTSTQEIVNSKGLNL
                     KKELKSCGAYAVAVASDGTSTQTGFGVEVDFNFKGLDKDKIANKAVERATRLLGAKPI
                     KSGKYPVIIEKEAFASLLEAFLSIFSGEAAIKKTTLLLGKEKTKIFDERVNIVEDPLV
                     ETSIYYNPFDDEGVATKKKHLIKDGVFQGFAHNLKTAKALKQDLTGNGYNKSVDFAYT
                     YLENGDAPLDDLVKSIDKGLLITNLQGLHSGLNPISGDFSLQSSGFYIEDGKISKPTS
                     LIVTSGNFFEMMNDIDKIGNDLELRNESSVNAPSVKFNGLQVSGE"
     gene            3733..4914
                     /gene="metK"
                     /locus_tag="PLQ22_03065"
     CDS             3733..4914
                     /gene="metK"
                     /locus_tag="PLQ22_03065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOH58974.1"
                     /translation="MKEKILFTSESVTEGHPDKVCDQIADAILDEIIRNSLEEPRVAC
                     EVAVTTGIVLVMGEITTDCYVDIQKIVRQVINDIGYDRGKIGFDGDNVGVLLAINEQS
                     KDISKGVDHNEEGTTGAGDQGIMFGYASNETEEYMPIAITLAHKLTKRLAEVRKNEKK
                     KILRPDGKSQVTVEYDAKGKLKRIDSIVVSTHHVADISQEELRKYVLENVIKVVIPKE
                     LLDKETKYYINPTGAFTEGGPKADSGLTGRKIIVDTYGGYAHHGGGSFSGKDPTKVDR
                     TGAYMARYIAKNLVASGVSPKLEVGLSYVIGRSEPTSLLIDTYGYNKYSYDLIIKIIR
                     KVFPLTPRGIIDYFDLFKPIYRQVSNYGHFGRLDLDLAWEKLDKVDEIKKYLENPSLL
                     D"
BASE COUNT         1953 a          560 c          984 g         1627 t
ORIGIN      
        1 cttatgttat tcgtcaaaat atcccaatag agggagagtc tagttacaca gaccttgttt
       61 ttggtgagat tactgttcca aataaagata tggaagataa catcttaatt aaatcagatg
      121 gtatgcctac atataatttt gctcatgtag ttgatgatca tttaatgcat gttacttatg
      181 tcattagagg aaatgaatat ttatcatcta cgccaaaata taatttatta tatgatgcat
      241 ttgggtggga aaaacctcaa tatattcact taacacaaat tatgaaagat gaaacaagaa
      301 agttaagtaa aagacatggc gatgctaact ttgatgactt tattagaaaa ggttatttgc
      361 ctcatgcaat tgtaaactat atcgctttat taggttggag tccgaaagga acacaagaaa
      421 aatttactat gcaagagtta attgaaaact tttcagttga aggtttatct aaatcaaata
      481 gtatttttga tgaaaagaag atggcatggt taaatggtga atatattaaa gagctaagtt
      541 ttgaagaatt ttataaatat gctttaccat attttgaaaa atctagcatt aagggtcgtt
      601 atgattatga accttttgcc agattacttc aatcaagaat tgaaatcttt agtgagattc
      661 cagaaaaggt aaagtttata gatgaatatc aaagatttcc aagtgagaaa tatgaaaatc
      721 aaagattcaa aattgatcaa gaacttgctt ataatatttt aaagagttat ttagaaaaag
      781 cagaaaaaat agacttttct gaagaagctt ggaaagaatt agttaaagaa gtagctgatt
      841 tatatcagat aaaacaagga gctgtttact ctacaatgag atgggcgatt tctggtgttg
      901 atgtaactcc aggtgggttt gtggagatag cagtaatatt aggaaaagaa aaatcaattg
      961 aaagattaaa gcaatcttta gcttggctaa aagaagattt aggaaagtaa aggggagatt
     1021 attatgaaat taacaaaaaa agattataaa gaaatattag aagcatgttt agaaacaggt
     1081 gctgattttg cggagattta tcttgaagat aagattgcta ataactatgc gatgattaac
     1141 gggaagttag atagagctaa tagtagtaat ctatacggag caagcattag aatcttacaa
     1201 ggagtagaag agtcatttgg ttatacttct gatttaagta aagaaaactt acttaatcta
     1261 gcaaggaagt taaatactag ttttaatgct ccaaagcaaa aaattaaatt tacgttaaaa
     1321 gaagaagaaa caccaaagat tatttttgtg gaaagaagcg ctagcaaaac taaactaaaa
     1381 gaaaagatta aattattagc aagagcagaa aaaggtttaa gaagctttga ttctaaagaa
     1441 atcattagaa ctaatgctgt attaacagat gaagaacaat atattacagt tattaatact
     1501 ttaggagtaa tcaaaaaaga tttaagaagt cacttacgtc tttcggtatc agctgttgca
     1561 tcagatggca aaaatatgca agatgcattt ggctcaaaag gtggtaactg tggttatgaa
     1621 ctatttgata attttgatat tgaaaaaatg ggatatgaga caagtgaaac tgcagttaaa
     1681 atgcttcatg ctgatgaaat gaaaagtggt aagatgactg tagttattga taatggattt
     1741 ggtggagtat tacttcatga agcttgtgtt cactcgttag aagcaacatc agtagctaaa
     1801 ggtagttcag tattctgcaa tatgcttgga aaacagatag catcacctat cgtaactgca
     1861 attgatgatg gtacgattcc aaatgcttgg ggatcattaa atattgatga tgaaggtaat
     1921 gagaccaaga gaaatgtttt aattaaagat ggtattttac aatcatactt agtagatttt
     1981 agaaataatc gtacgatgaa acatggtatt actggtagtg gtagaagaca aaacttcaaa
     2041 tattcaccaa catcaagaat gactaacaca ttctttgctc ctggagattc tacatttgaa
     2101 gagattatta aaaatacaga atctggattc tttgctaaga gtatgggtgg tggaagtgtt
     2161 aacccagcaa ctggagagtt taactttgca gttaatgaag gttatttaat tgaaaatggt
     2221 aagattacaa aaccagtaag aggagctaca ttagtaggta gtggtgctga gattttagct
     2281 aatattgata tgattgcaaa taacttagag ttctcttatg gtatgtgtgg atctttaagt
     2341 ggacaaatcc caacaatcgt gggtcaacca acaattagag ttcaaaatat cactgttggt
     2401 ggaaagggga attaagatgg attataagaa aatattagaa caaggtaaaa agttaggctt
     2461 ttctgattta gaatttcaaa tttcaagtag cacatcttta tcaattgatg tatttaaagg
     2521 aaaggtagat aagaaccaag ttcatagtga tttttcaatt gttctaagtg gtttatataa
     2581 agatcaactt gcctctgttt cgacaacaaa caaagatttt gatcctaaga aactcttaaa
     2641 gaaactaaaa gaaaatgcta aggctttaaa taaagacgaa aaagaagaaa tctttgctgg
     2701 tagtaaagaa tatccgaaat caattgttca taaagttgat tttaataata tagataataa
     2761 gaaaaaagtt gattttcttt tagatttaga aaaaaaagtt tatgactttg ataagagaat
     2821 tactttagta tctaattgta gatatttcga atcaactagt acacaagaga ttgttaatag
     2881 taaaggatta aatcttaaaa aagaattaaa atcttgtggt gcttatgcag ttgctgttgc
     2941 aagtgatggt acaagtacac aaacaggatt tggagttgaa gtagatttta attttaaagg
     3001 tttagataaa gataagattg caaataaggc tgtagaaaga gcaacaagat tattaggagc
     3061 taaaccaatt aaatcaggta aatatccagt aattattgaa aaagaagctt ttgcatcatt
     3121 actagaagct tttcttagta ttttcagtgg agaagctgca attaagaaga caacactttt
     3181 acttggaaaa gaaaaaacaa agatttttga tgaaagagtt aatattgtag aagatccatt
     3241 agttgaaact tctatatatt acaatccttt tgatgatgaa ggtgttgcta ctaagaaaaa
     3301 acatcttatt aaagatgggg tatttcaagg ttttgctcat aatctaaaaa cagctaaagc
     3361 tttaaaacaa gatttaactg gaaatggtta taataagagt gttgactttg cttatactta
     3421 tttagaaaat ggagatgctc ctttagatga tttagtaaaa agcattgata aaggactact
     3481 gattactaac ttgcaaggat tacattcagg attaaatcct atttcaggcg attttagttt
     3541 acaatcatct ggattttata ttgaagatgg taagattagt aaaccaacta gtttaattgt
     3601 tacatcaggt aacttctttg agatgatgaa tgatattgat aaaattggca atgatttaga
     3661 actacgtaat gaaagttcag taaatgcacc ttcggttaaa tttaatggtt tacaagtttc
     3721 tggagagtaa aaatgaaaga gaaaatttta tttacatctg agtcagttac tgaaggacat
     3781 ccagataaag tttgtgacca aattgctgat gcgattttag atgaaataat tagaaattct
     3841 ttagaagaac caagagttgc ttgcgaagta gctgttacta cgggaatagt tttagtaatg
     3901 ggagaaatta ctactgattg ttacgtagat attcaaaaaa ttgttagaca agtaattaat
     3961 gatatcggtt atgatcgtgg caagattggt tttgatggtg ataatgtagg tgttttactt
     4021 gcaattaacg aacaatcaaa agatattagt aaaggcgtag atcataacga agaaggaact
     4081 actggagctg gtgatcaagg aataatgttt ggttatgctt caaatgaaac cgaagaatat
     4141 atgccaatcg ctattacact tgctcataag ttaactaaaa gattagctga agtaagaaaa
     4201 aacgagaaaa agaaaatctt gcgtcctgat ggtaaaagtc aagttacggt agaatatgat
     4261 gctaaaggta aattaaaaag aattgatagt attgtagttt caactcacca tgttgccgat
     4321 attagtcaag aagagttgag aaaatatgta ttagaaaatg taattaaggt agttattcca
     4381 aaagagttat tggataaaga aactaagtat tacattaatc ctacaggagc ttttactgaa
     4441 ggaggtccta aagctgatag tggacttact ggaagaaaga ttattgtaga tacatatggt
     4501 ggatatgctc atcatggagg cggttcattt agtggtaaag atccaacaaa agtagatcgc
     4561 actggagctt atatggcaag atatattgcc aagaatttag ttgcatcagg tgtttctcct
     4621 aaacttgaag taggattaag ttatgtaatt ggcagaagcg agcctacatc attattaatt
     4681 gatacttatg gttataataa atattcatat gacttaatta ttaaaatcat tagaaaagtt
     4741 ttcccattaa caccaagagg tattattgat tattttgatt tatttaaacc aatttatcgc
     4801 caagtgtcta attatggcca ttttggtaga ttggatttag atcttgcttg ggaaaagtta
     4861 gataaagttg atgaaataaa gaaatattta gaaaatccta gtctattaga ctaggatttt
     4921 ttataatacc attttaatag ccaaatgtta ggttaaaatt gactttttta taaaaataat
     4981 gtataatatg tttgtaaacg tttgtgttgg caaataataa ataaggagga taactattaa
     5041 acagagccaa catttatttg tttaatagta aataaaagat gagaaaattt ggaaaaattt
     5101 tagtagctct tgtattaatg ctag
//
LOCUS       DAOSGS010000059         5105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14539_length_5105_cov_5.525941, whole genome shotgun sequence.
ACCESSION   DAOSGS010000059 DAOSGS010000000
VERSION     DAOSGS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14539_length_5105_cov_5.525941"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..745
                     /locus_tag="PLQ22_03070"
     CDS             407..745
                     /locus_tag="PLQ22_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH58975.1"
                     /translation="MEYTSKEKEVISQIGKFFLERNLKKSDNETKLHQVYLQTREEIQ
                     DLCITRIIFTSDRSIDIYLGRPGLLIGKKGEQIDALSEYINKKYGINKIQIREDNLFM
                     YLIPNDWPGY"
     gene            1508..1753
                     /locus_tag="PLQ22_03075"
     CDS             1508..1753
                     /locus_tag="PLQ22_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58976.1"
                     /translation="MKFIYLITILLMLISCSVENENVNNINQPNGTIVTDNRGPQLRT
                     IENELYKKLKEEAENEEKKIKTAQKKDTVRNGSYSQK"
     gene            complement(1845..2162)
                     /locus_tag="PLQ22_03080"
     CDS             complement(1845..2162)
                     /locus_tag="PLQ22_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH58977.1"
                     /translation="MLNLTHDTFAETIFTTNKPFFVQFSADWCSVCKDLHPMIEQLEQ
                     EYQNQVTFVKIDVDTDADLADQFKIDKLPTFLLFKDREIVDCIVGAESLNHFKTKLNE
                     VVE"
     gene            complement(2143..2274)
                     /locus_tag="PLQ22_03085"
     CDS             complement(2143..2274)
                     /locus_tag="PLQ22_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58978.1"
                     /translation="MTRDELIKYYVEQIKEHDKLIEITDAEVVQTEEVIDQDAQPNT"
     gene            complement(2342..>2553)
                     /locus_tag="PLQ22_03090"
     CDS             complement(2342..>2553)
                     /locus_tag="PLQ22_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58979.1"
                     /translation="FDKQSPGFVIEQLTNSKDFTNTQDELDEEFKHPENWFDEFYDEA
                     GASFTYDELEKIGNLYENSDLLPND"
     assembly_gap    2554..2653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2675..2881)
                     /locus_tag="PLQ22_03095"
     CDS             complement(2675..2881)
                     /locus_tag="PLQ22_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58980.1"
                     /translation="MLKQLKPDRLYFIINIDESYASRIFEVLKEEETKRGNWPEGDIS
                     YREWVEQTFGQAGLDVLDKVKSDI"
     gene            complement(2903..4639)
                     /locus_tag="PLQ22_03100"
     CDS             complement(2903..4639)
                     /locus_tag="PLQ22_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58981.1"
                     /translation="MELAITNMFIKDLQFFGIMRSSITKDFHDVKLKIEFYKILTSDR
                     QLVEMVEKSCLEDHVNPNALKLVSSELVYTKVLNYNDSEYFRNYLSFVCSRNLYEDRK
                     ANKVWIIEFKFPMSGDVNFFRINLTTDQVTRLYSQINSFYSNFMLFSAIAKQHIVDQP
                     KEEVQTKKVTEYAKEAENTAIEIETQKVSQLAPQTKERTELDDIFELCLNSSIKSSLI
                     HYTFNYFKYFTKDHCKIQTNQKGQVQYILPLLIPGNLNFDNSYFDSLIQANGSVSSSN
                     VIFAIKKILNNLLLNHSKYHEKPMIIMMMSYLLFVYMLKYLNDTNKDSFRTHFRRLVC
                     EADSQSGILRSLIATSTFKDFTNKIFFKIVNICPAEAVNDQEIERINVLSQEYKHLIP
                     VSPEEFVEKVLNKANNLNDDKMPKFFKLSNKKVLDISKYISPKEQPTSYSTVLFDPDQ
                     TLDHDKSEDKLYNILNYELINNGTRVISKAYQHLINYVKNPSSLLLLKETDLPKEVIN
                     LFDILSKKILNPSVISKDNVNTLNYYNVLVESLPNSNQFNHITMMYIIFQIMIPYHYD
                     IYHTNQFTDCLL"
     gene            4883..>5105
                     /locus_tag="PLQ22_03105"
     CDS             4883..>5105
                     /locus_tag="PLQ22_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58982.1"
                     /translation="MKKLCSITIKGKTKTWTFNTFVDLKYIDEWREDGIEIDPIENVI
                     PVWVVDMRLTRIWCFLQDVWHGKYLRKLGW"
BASE COUNT         1766 a          729 c          751 g         1759 t
ORIGIN      
        1 gaaaaagtaa gatattttca tttgtaatat atatccacag atcaaatcct gccgatagat
       61 ttgttatgat atttaaaaat cttatataga aataataata taaaatgaaa ggagattatc
      121 atgttaacat gttctatctg ctgtgaaacc agcgaagtta gaatggttaa agttaatatt
      181 ggtaagggaa gaactcaaga aatcccttac tgcaaaagac atgctgctca tttagaacca
      241 acatgtcaaa cagaacaaaa aggtgaagat gatatcgtcg cttactgttg tgggcgacca
      301 gtaaatccaa aaacaggtcg ctgcagtaca tgcggcgaca ggtacgatta acacattggc
      361 aggatcttta actagatctt gccgatattt ttaaaggaga aaattgatgg agtatactag
      421 caaagaaaaa gaagttattt ctcaaattgg aaaattcttt ctagaacgca atttgaaaaa
      481 atctgataat gaaacaaaat tacaccaagt ttatttacag acaagagaag agattcaaga
      541 cttgtgtatt acaagaatta tattcacatc agatcgatcg atagatattt atttgggccg
      601 accaggttta cttatcggta agaaaggaga acaaattgac gctctctcag aatacattaa
      661 taagaaatat ggaattaata aaatccaaat aagggaagac aatttgttta tgtatttgat
      721 tccgaatgat tggccgggat attaatcata ttttaaccgg caagatctaa tttaaagatc
      781 ttgccgaatt tttatattca aagattttat atagaaataa tattaacatg ttaccgataa
      841 cagtaagtat cggggacctc gctgtgagat ggataacagc gtgagcctat cgacggggca
      901 agaggagaaa tccgtccagc ggtacgagct tcgaaagaag tgggtgctaa agccgcatat
      961 atgtgggggc cgcgccaccc tcacattttt attttcaaag atttcatata gaaatataat
     1021 tataatttct atatattgcg ctttttttct gatattcaaa gattttatat agaaataata
     1081 gtagtggaca tagcaatatg tcccatgggt ggtagagatt aagtcggtct ctagccctcc
     1141 cctagcttca agcgtagggg tgctcgcccc gcgtttgaag aaatgtttaa tctattaact
     1201 tcggttaatg gattagacat ttcttcttgt ttttaaactt ttcatataga aatattatat
     1261 ttaatatcag agctattaat ggctctgttg aactggaaag gagacaaaaa atgataataa
     1321 atataatact attcgttaca ggtacagtcg tccttgcggc gatatctggc gatccgaata
     1381 tgattggatt agccagcgca ttgttggtag cggcttcata ttaaattaaa aatcctcttc
     1441 ggaggatttt tcttttttta ctaatttcat atagaaataa tattttaaaa aactaaagga
     1501 gaatataatg aaattcatat atttgataac tattttatta atgctcataa gttgtagtgt
     1561 agaaaacgaa aatgttaata atatcaacca accaaatgga acaattgtta cagataatcg
     1621 tggaccacaa ttaagaacaa ttgaaaatga gctatataaa aaattaaagg aggaggccga
     1681 gaatgaagaa aagaaaatta aaactgcaca aaagaaagat accgttagaa atggctctta
     1741 ttctcagaag taagcattct gtaaaagaga gcaaaaaaat ctataatcga aacaaaaagt
     1801 tcccgtttga tgataataaa aataattatc atcaaacgga ttttttattc gacaacttca
     1861 ttgagcttgg ttttaaagtg attaagagat tctgctccaa ctatacaatc tacaatttcc
     1921 ctatctttga ataataggaa agttggtaat ttatctattt taaattgatc ggcaagatct
     1981 gcatcagtgt caacatcaat ctttacaaaa gtaacttgat tttggtattc ctgttctagc
     2041 tgttcaatca ttggatgaag atctttacaa actgaacacc aatcagcact aaattgaaca
     2101 aaaaaaggtt tgtttgtggt gaaaattgtt tctgcaaacg tatcatgtgt taggttgagc
     2161 atcttgatca ataacctcct cagtttgaac tacttctgca tctgttatct caattaattt
     2221 atcatgttct tttatctgtt ctacataata cttaattaat tcatcccttg tcatgtttat
     2281 atccttttat agaaataaaa aacgtctctt gccgataaaa taattatcgg caagatcatt
     2341 tttaatcgtt tggaagtaaa tctgaatttt catacaaatt cccaattttt tcaagttcat
     2401 catatgtaaa acttgcacct gcctcgtcat agaattcatc aaaccaattt tcgggatgtt
     2461 taaactcttc atctaattca tcttgagtat tagtaaaatc tttactatta gttaattgtt
     2521 caattacaaa tcctggtgat tgtttatcaa aagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnaaatatt tctatataaa atctttaaat atcagatttt actttatcga
     2701 gaacgtcgag accagcttga ccaaatgtct gttctaccca ttccctataa gaaatatcac
     2761 cctccggcca attacccctt ttagtttctt cttcttttag aacttcgaaa attcttgaag
     2821 cataactttc gtcaatatta ataatgaaat ataaacgatc tggtttaagt tgtttgagca
     2881 tatatttgtg ccttcctttg tattagagta gacaatctgt aaattgatta gtatgatata
     2941 tatcataatg atatggtatc ataatctgaa aaattatata catcatggtt atatgattaa
     3001 attgatttga attcggcaag ctctcaacaa gaacattata ataatttaaa gtattaacat
     3061 tatccttgct aatgaccgat gggtttaaga ttttcttcga taatatatca aatagattaa
     3121 tgacctcttt tggtaaatct gtttctttta ataacaataa tgagcttgga ttctttacat
     3181 aattaattaa atgttgatac gcttttgata taactcgtgt cccattattt atcaactcat
     3241 aatttaaaat attatataat ttatcttcag atttatcatg atcaagagtc tgatcaggat
     3301 cgaacaacac tgtagaatat gaagttggtt gttctttcgg agatatatat tttgaaatgt
     3361 ctaatacttt tttatttgat aatttaaaaa atttaggcat tttatcatca tttaaattgt
     3421 tagctttgtt taatactttt tcaacaaatt cttctggaga tactggaata agatgtttat
     3481 attcctgtga gagaacattt attctttcaa tttcctgatc gtttacagct tctgccggac
     3541 aaatattaac aatcttaaag aatatcttat ttgtaaaatc tttaaaagtc gatgttgcaa
     3601 tcaaagatct taatattcca gattgtgaat ctgcttcaca taccaatctt cgaaaatgag
     3661 ttctgaatga atctttattt gtatcattta agtattttaa catataaaca aataataaat
     3721 aactcatcat cataataatc ataggctttt catgatattt actatgattt agaagtaagt
     3781 tatttaaaat ctttttaata gcaaatataa cattactgga tgaaacagaa ccatttgctt
     3841 gaattaatga gtcaaaatat gaattatcaa aatttaaatt accaggaata agtaaaggta
     3901 atatatactg aacttgtccc ttttggtttg tctgaatttt gcaatgatct tttgtaaaat
     3961 acttaaaata attgaaagta tagtgaatta gactagattt aattgaacta tttaaacaca
     4021 attcaaaaat atcatccaat tctgttcttt cttttgtttg cggcgcgagc tgagaaacct
     4081 tctgggtttc tatttcaatt gctgtatttt cagcctcttt tgcatattca gtcactttct
     4141 tagtctggac ttcttcttta ggctggtcta caatatgttg tttagctatc gccgaaaaca
     4201 acataaaatt tgaataaaag gaattaattt gactatataa cctcgttact tgatctgtag
     4261 ttaaattaat cctaaagaaa ttaacatctc cagacatagg aaatttaaat tctataatcc
     4321 aaactttatt agcttttcga tcttcatata aatttctaga gcatacgaaa cttaaataat
     4381 ttctaaaata ttcagaatca ttataattca acactttagt gtatactaac tcgctactaa
     4441 ctaattttaa tgcgtttgga tttacatgat cttctaaaca gcttttctca accatttcaa
     4501 ctagctgtcg atcagatgtt aaaatcttat aaaattcaat ttttaattta acatcatgaa
     4561 aatcttttgt aatgctactt ctcattatac caaaaaattg taaatcctta ataaacatat
     4621 ttgtaattgc tagttccatt atgcctccat tacatattta tgaaaggtca cagaagaaaa
     4681 cgttattgaa aatgagatag acatattata tgtctgtaac gttcttctta ggacctttcg
     4741 tatatattat ttctatatat ttttagaaaa aaattatttg tatttccagc tcataaaaat
     4801 ttttattttg ctaaatttca tatagaaata atatatttaa tatttttaat ttttatcaaa
     4861 aaaaaggagt ataatatcac acatgaagaa gctatgctca attacaatta aaggaaagac
     4921 caaaacgtgg acatttaata cttttgtaga tttaaagtat attgatgaat ggagagaaga
     4981 tggaattgaa atagatccaa ttgaaaatgt tattccagtt tgggttgttg atatgagatt
     5041 gacacgcatc tggtgttttc tacaagacgt ttggcatggt aaatatttac ggaaattagg
     5101 ttggt
//
LOCUS       DAOSGS010000060         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14656_length_5075_cov_5.352988, whole genome shotgun sequence.
ACCESSION   DAOSGS010000060 DAOSGS010000000
VERSION     DAOSGS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14656_length_5075_cov_5.352988"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..543
                     /locus_tag="PLQ22_03110"
     CDS             196..543
                     /locus_tag="PLQ22_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58983.1"
                     /translation="MEYEKTILELLERVVTLEEKVAVLEGNLANRGAKPARGKYTEMV
                     IDYINRKIEKAKKQGLNNITLTSGNIQKDVGLKNRLPLVCNAMRKCMDDKSEIVYETP
                     SGQSSTFTIKWNF"
     gene            557..2296
                     /locus_tag="PLQ22_03115"
     CDS             557..2296
                     /locus_tag="PLQ22_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOH58984.1"
                     /translation="MPTSNKYEDMSALTALKYAKDKNLLLPDIQREYVWDIYEIESLF
                     ESFVDDYPIGSCIFWKTNRKTINEEKPNLYYFIRKFKKGETKNEKASEVLTDECDYYI
                     VLDGQQRITSMNIALYGSYTYYKGGRGHNRSNPNSWVTKELYYNLDYYAVKEEDDEVP
                     RKRFCFLTDGEVANGNYYKVKLMLAYDALDSFLEFMIEKQFKKQCRSDLSILFQRLHD
                     SSGNGLVHYYCIAENTYDEALDIFVRVNSTGRKLSKSDLLFSTLIDGWKDGKDNIENL
                     LATMNSKGDGFQFSRDYLMRLCLVLVDANTNLKINSLTQDTIQKIRDNWNKINIALDS
                     MSTMLSEIGICHENLTSYNATMPIAYYLFKGGKIKDKDAKKETRKFLSVSLAKRLFGV
                     ASNDALNSTRNALKDLDCKHTPFSLDLFASISLTGGRTFTVGESDIDYWLDTYEKGQS
                     TFVLLSLLYPNLKLSQVSFHQDHCHPYAGFENKVISSLGLTDEKVKEWQKKRNLLPNL
                     QFLEGAENESKNKTPLIDWVKEGNNFEYHLSDVSLELKDFDEFFKQRRALIRSELLFI
                     FGIHDQNTMEELV"
     gene            2305..2571
                     /locus_tag="PLQ22_03120"
     CDS             2305..2571
                     /locus_tag="PLQ22_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HOH58985.1"
                     /translation="MARKLKIYKQSTSGTNYTQVPTIILKGQWLKEAGFNFGEYVEVI
                     CEGENITLTKTEAPVIKEELSLEDKINNLNEDQKLKLSKLIDKL"
     gene            2586..3458
                     /locus_tag="PLQ22_03125"
     CDS             2586..3458
                     /locus_tag="PLQ22_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58986.1"
                     /translation="MIETISQKIERFYSLIRNYQEGAPETRYKSWEWCHKAFLDNKEN
                     TEEGNIEFLSLHLAFYLASWGMYRGSSYLLQRDYKAHKAAVREIIKPEYSSLWDYIPT
                     ANNIESTNDLLFNTENGLYWKIKNSYKGFDDETEEDESSDTLTTKILMGTFGCVPAFD
                     RFLKSGIKCYQDNHDGRNTRIGNYKLTQSIEKAGGTAATESFKALARFAVEHRTELTV
                     DTNFLYSPMKCVDMYFWEIGYEMDIAALLKNEKTKDHVKQKLYKKAVKLNYCNEDITY
                     EEAIAQIRRLNYNE"
     gene            3543..3953
                     /locus_tag="PLQ22_03130"
     CDS             3543..3953
                     /locus_tag="PLQ22_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOH58987.1"
                     /translation="MLPPHVLRKYVFPWYQKIVKLAHDKGKLAIVHSCGYYHDIIDDM
                     VDTIQFDGKHSFEDNIYPVEKAYQDLKERIAILGGLDMNFLAHKSSEEVYQRSKNMLA
                     LTKQGGYALGSGNSIPDYIPSENYLAMLQAGIEK"
     gene            complement(3976..4680)
                     /locus_tag="PLQ22_03135"
     CDS             complement(3976..4680)
                     /locus_tag="PLQ22_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58988.1"
                     /translation="MNWKRLQKNKNLTTILYLTTRILVFAILAFAIVQMVKEHNYEYD
                     SRNAFIITQCVILLTYSFIPMIIEKQFKVEIPEAMEVLFIFFGIGSLLIGEIIGAYQM
                     NSWWDGVMHFMGGSLIAIFSFSLINLLHRSTTFHINLNPFFIVLFAFCCSMTLAVIWE
                     MIEFSIDSTTLSNMQRYRDSITGEPFIGQAALNDTMYDFILGSLGAFVVCTLEYFKLK
                     YKHKFSMMIEPIHPKE"
     gene            complement(4879..>5075)
                     /locus_tag="PLQ22_03140"
     CDS             complement(4879..>5075)
                     /locus_tag="PLQ22_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58989.1"
                     /translation="NREPNAIKDSYKKRTQKLNPLKPKVASTACANQLVRIIFYMHKT
                     SSTYKYICKQSQTVSTVTLL"
BASE COUNT         1909 a          683 c          893 g         1590 t
ORIGIN      
        1 ggcaattgtt aataaagaca aaaccataac ctttaagttc acaagtggaa ctgaagtaac
       61 attgtaagat tagggccttg actcaaggtt ctttttttat tgtattgctt atagaaatgc
      121 aaagaaatgc atattgcata atttgacata tgcaatgtat tagtgtataa ttgtatttat
      181 tggaggcgtt tttatatgga atatgaaaag accatacttg aattacttga aagagttgtt
      241 actttagaag aaaaggtggc ggttttagaa ggtaatcttg ctaatagggg tgcaaaacct
      301 gctagaggta aatatactga aatggttatt gattatataa acaggaaaat agaaaaagca
      361 aagaaacaag gtttaaataa cattacttta acttcaggca atatacaaaa agatgtgggt
      421 ttaaaaaatc gtttacctct tgtttgtaat gcaatgagaa aatgtatgga tgataaatcg
      481 gaaattgtct atgaaacacc atcagggcag tcttcaacat ttacaatcaa gtggaatttt
      541 taaaggagaa tgaacaatgc ctacatcaaa caaatatgaa gatatgagtg cattaacggc
      601 actaaagtat gctaaagata aaaaccttct tttaccagat attcaaagag aatatgtgtg
      661 ggatatttat gaaatagaaa gcttgtttga atcgtttgtg gatgattatc caatcggatc
      721 atgcattttt tggaaaacca atagaaaaac aattaatgaa gaaaaaccta atttatatta
      781 ttttattcgt aagtttaaaa aaggtgaaac caaaaatgag aaggcatctg aagtattaac
      841 tgatgaatgt gattactata ttgtccttga tggacaacaa agaataactt caatgaatat
      901 agccttatat ggttcataca catattataa gggcggtaga gggcataatc gctcaaatcc
      961 taattcatgg gttacaaaag aactttacta taatttagat tactatgcag taaaagagga
     1021 agatgatgaa gttcctagaa agagattttg ttttttaaca gatggcgaag tagcgaatgg
     1081 taattattat aaagttaaat taatgcttgc atatgatgct ttggatagtt tccttgaatt
     1141 tatgatagaa aaacaattca aaaagcaatg ccgttctgac ttgtccatat tattccaaag
     1201 attacatgac tcatcgggta atggattagt acattattat tgcattgcag aaaatacata
     1261 tgatgaagca ttagatattt ttgtcagagt gaattcaaca ggaagaaaac tttccaaatc
     1321 agatctactt ttctcaactc ttatcgatgg ttggaaagat ggaaaggata acatcgagaa
     1381 cttattagcg actatgaata gcaagggcga tggatttcaa ttttctaggg attatttaat
     1441 gagattgtgt ttagttttag ttgatgcaaa tacaaattta aaaattaatt ctttgactca
     1501 agatacgatt caaaaaatac gtgataattg gaataaaata aatatcgcat tagattcaat
     1561 gtcgacaatg ctttctgaaa taggcatttg tcatgaaaat ttaacatcat ataatgctac
     1621 tatgccaatt gcttactatt tatttaaggg tggaaagatt aaagataaag atgcaaagaa
     1681 ggaaaccaga aaattcttgt cagtatcatt ggcaaagaga ttgtttggtg ttgctagcaa
     1741 tgatgcatta aattctacta gaaatgcatt aaaagatctt gattgtaagc atactccttt
     1801 ctcgttggat ttatttgcaa gtattagttt aacaggtggt agaactttta ctgtcggtga
     1861 aagtgacatt gattattggc ttgatacata tgaaaaggga cagagtacat ttgttctttt
     1921 gtctttgctt tatccaaatt taaagttaag tcaagtatcc ttccatcaag atcattgtca
     1981 tccatatgcg ggatttgaaa ataaagttat atcttcactt ggtttaactg atgaaaaagt
     2041 taaggagtgg cagaaaaaaa gaaacttatt acctaatctt caatttttag agggtgctga
     2101 aaatgaaagc aaaaataaaa cacctctaat agattgggtg aaagaaggaa ataactttga
     2161 atatcatcta tctgatgttt cgcttgaact taaagatttt gatgaattct ttaagcaaag
     2221 aagagctttg attagatcag aacttttatt tatctttggt atacatgatc aaaacactat
     2281 ggaagaatta gtgtaggtgt aattatggct agaaaactaa agatttataa acaatcaacg
     2341 agtggaacaa attatactca agtacctaca ataattctaa aagggcagtg gctaaaagaa
     2401 gcaggtttta attttgggga gtatgttgaa gtaatatgtg aaggtgaaaa tattacttta
     2461 accaaaacag aagcacctgt gattaaagaa gaactttcac tagaagataa aattaacaac
     2521 ctaaatgaag accaaaagtt aaagttatca aaactgatag acaagttgta aattggagga
     2581 actctatgat agaaacgatt tcacaaaaga tagaaagatt ttattcactt attagaaact
     2641 atcaagaagg tgcacccgaa acaagatata aatcatggga atggtgccat aaagcttttt
     2701 tagataataa agaaaatact gaagaaggca acatagaatt cttatcgctt catttagctt
     2761 tttatttagc tagttgggga atgtatcgtg gatcgtcata tttacttcaa agagattata
     2821 aggctcataa ggctgctgtt cgagaaataa ttaaacctga atattcttct ctatgggact
     2881 acattcctac agctaataat attgaatcga ctaatgattt attatttaat accgaaaatg
     2941 gactttattg gaaaattaag aattcttata aaggctttga tgacgaaaca gaagaagatg
     3001 aatcttctga tactttaaca acaaaaattc taatgggaac atttggctgc gttcctgcat
     3061 ttgatagatt cctgaaatct ggaattaaat gctatcaaga caatcatgat ggaagaaata
     3121 cacgaattgg taattacaag cttactcaat caatagaaaa agctggtggg acggcagcaa
     3181 ctgaaagttt caaagcactt gcaagatttg ctgtagaaca tagaactgag cttactgttg
     3241 atactaattt cctttattcg ccaatgaaat gcgttgatat gtacttctgg gaaattggat
     3301 atgaaatgga tatagcagcg ttacttaaaa atgaaaaaac aaaagatcat gttaagcaaa
     3361 agttatataa gaaagctgtc aaattgaatt attgcaacga agatataaca tatgaagaag
     3421 caatagctca aatcagacga ttaaactata acgaataaaa aataggggta tgcaaaaact
     3481 ctcaaaggta tgcaattttg ccttagggta tgcattttta ttaaaatagg tagttatagc
     3541 caatgttgcc accccatgtt ttacgaaaat atgtatttcc ttggtatcaa aaaattgtaa
     3601 aattagctca cgataaagga aaattagcta tagttcattc ttgtggatat tatcatgata
     3661 ttattgatga tatggttgat accatccaat ttgatggaaa acactctttt gaagataata
     3721 tttatccagt agaaaaagca tatcaagatt taaaagaaag aattgccatt cttggaggtc
     3781 tcgatatgaa tttcttagcc cataaaagta gcgaggaagt atatcaaaga agtaaaaaca
     3841 tgctagcact aaccaaacaa gggggctatg ctttaggaag tggaaatagt attcctgatt
     3901 atattccaag tgaaaactat cttgccatgt tacaagcagg aatagaaaaa taaaacagct
     3961 gaaaaatcag ctgttttatt ctttaggatg aatgggttca atcatcatac taaatttatg
     4021 tttatatttt aatttaaaat actctaaagt acaaacgaca aaagctccta aactacctaa
     4081 aataaaatca tacatggtat cgtttagagc agcttgaccg atgaaaggct ctcctgtaat
     4141 cgaatcacga tatcgttgca tattgctgag tgttgtacta tcaatagaaa actcaatcat
     4201 ttcccaaatg actgctagtg tcatagaaca acaaaaagca aataatacaa taaagaaagg
     4261 atttaggtta atatgaaaag tagtgcttcg atgaagcaag ttgatgagtg aaaacgaaaa
     4321 aatagcaatt aaacttcctc ccataaaatg cataactcca tcccaccagg aattcatctg
     4381 ataagcacca ataatttctc caattaataa agaaccaatt ccaaagaaaa tgaataaaac
     4441 ttccattgct tctggaattt caactttgaa ttgtttttcg ataatcattg gaataaagga
     4501 ataggttagt agaatcacgc actgagtaat aataaaagca ttacgactat cgtattcata
     4561 gttatgctct ttcaccattt ggacaatagc aaaagctaag atggcaaaaa ctaaaattct
     4621 tgtcgttaag tataaaatag tggttaagtt tttattcttt tgtaatctct tccagttcat
     4681 aatatcacct aatctattat aaaccattat gagtaaataa tcaagttaat taataaagtt
     4741 ttttcataac ttatctttat cacttgatta tattaaaaaa gtgcacaata aaacgcatca
     4801 gtctaaaacc gataaactga cgttttcgca ctacttttta aaaatatcta aaatattcta
     4861 tttacagcta aaattgtgtt ataataaggt aacggtggaa accgtttggg actgcttaca
     4921 aatatatttg taagttgagc tcgtcttgtg catatagaag attattctta ctagttgatt
     4981 ggcacaagca gtggacgcaa ctttaggctt gagtggatta agcttttgcg tccttttttt
     5041 ataactatct ttaatagcgt ttggctcccg attgg
//
LOCUS       DAOSGS010000061         5032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_14837_length_5032_cov_5.423548, whole genome shotgun sequence.
ACCESSION   DAOSGS010000061 DAOSGS010000000
VERSION     DAOSGS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14837_length_5032_cov_5.423548"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLQ22_03145"
     CDS             complement(<1..1204)
                     /locus_tag="PLQ22_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOH58990.1"
                     /translation="MKISKTLFKELIRCESYYSLNKLYEEKRNASFVNGSLFDLQKYL
                     DEMIDKNTYENKLENPMKEYEQEQFAETERVLIKYANKILKTNLKAGSLAEQKEVVYH
                     DQEGNTFYTKIDGYQETINEIIVLEAKATTTNKFIDEKKPFFEKEESILRLKERSELE
                     TEDEIETYDKFLVDQVFNPTSALGKYIFDGLYTYWLVTKNNPHLKKISFYLAFLNHEY
                     TLDLNRATVDDPYPYVEGKGEIVTLLEMGQFYNEYLYLLEAEVKKIQSAIKQNVFIQP
                     EIDSKCKRNDNSEECLFCPVCYAYLENIPGSALYFAGKKKYKDILEGKITMDMYSDSE
                     LTSVLHKIQREAFIKDEQHINKEAIKKWLDKLEYPIYYLDFETLSSPLPRFQDETPYM
                     QSVFQFSLH"
     gene            complement(1265..2008)
                     /locus_tag="PLQ22_03150"
     CDS             complement(1265..2008)
                     /locus_tag="PLQ22_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH58991.1"
                     /translation="MNKIDKKYLYYSLGILFVIFVWFIGSILVDSEIVLPKISLVFKT
                     IGELFTKGETYLIILRTFLDILIVTILTLILAFVFSLLALRFEGFKHFFSPIVTLLKS
                     IPVVAVIVLFLLMVGQRFTPYVITFLVIFPILYEGIYSSINSIDKDLLDDVKTVSNLN
                     YLVIKDIYFPLSKPKILVSLLQSFGLGLKVIVTAQMIAQTKGTIGYQIAIQNTYLETK
                     GVFAWTIILIIFVFIIDFILHLIIKKNKR"
     gene            complement(2001..2927)
                     /locus_tag="PLQ22_03155"
     CDS             complement(2001..2927)
                     /locus_tag="PLQ22_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH58992.1"
                     /translation="MKRVVSFFLLLFSFVFLTGCTEDAKLKVTLPNGIPLISVAGTLT
                     EDIEYNNVTGPNLLVTAFTSKEADIIIAPINLGAKLYNSGNSSYKLDSVIGFLNTYIV
                     SIDEPLTSLADLDGKTLAAFGETNIPGITLRCALASENVNPNITYYNAINDGVAFFLK
                     GDYQYLLTSEPVLSNLRKKVSNLKYLSIADVLYKTTELDLVPQAAIFVNSESKNTKKI
                     AKFITAVENNITNLNRDPQGYANFIYQKDEYLESMKEDLIASSIPLSNIEFKKAIDIK
                     EDIEKFFNLLLTEDAALVGGKLPDQAFYTPYE"
     gene            complement(2927..4699)
                     /locus_tag="PLQ22_03160"
     CDS             complement(2927..4699)
                     /locus_tag="PLQ22_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOH58993.1"
                     /translation="MSNVKIWINNQEMTVPSTYTVLDAANDADIYIPRLCFLKDISET
                     SACRLCVVEIEGLNTLKNSCTVQVWEGMKVHTNTKRVNDAVKNNLKLLAANHHFECWV
                     CHREENCELLELLRRYNVPNTMGEDPTFSKKTSYRNISNSLTIDTSKCILCGRCVSAC
                     NHYTGLGILDFNRRGFDTYVAPANNDFIDDAGCLYCGKCIQACPVGALSGTSDEDIVI
                     DYLNNDNYYVIAQVAPAVRAALGEEFGNPIGTNVEGKIYRALKKMGFDEITDVNFAAD
                     VTIMEEGTELIGRIQDRLQGKEAHLPLFTSCSPGWIRYIERYYPEYLPHLSTAKSPQQ
                     IQGALIKHIYADIINVPKERIKVVSFMPCIAKKDEADREQMKDRGVKDVDAVLTTREF
                     ARLIRRFGINFNNLQDYKPESPLVTYTGAGAIFGTTGGVMEAALRTVKAFLEKEDKHT
                     IDFTQVRGSVAIKEADLVIDDQTFKVAVVHGAANFPEMFRRIKENPNKYIFVEFMGCE
                     GGCINGGGQPIVKARDLETIDVCKLRAQALYTIDSKNKIRRSHENPNVVSLYQTHLGK
                     PGGELAHKLLHTTYSEKEVFGEKK"
     gene            complement(4712..>5032)
                     /locus_tag="PLQ22_03165"
     CDS             complement(4712..>5032)
                     /locus_tag="PLQ22_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH58994.1"
                     /translation="LALMIKDTSLCALGQSAPNPVLSTLDKFYDEYLEHVVNKRCPAG
                     VCKNITKLLITDECIGCGKCLRNCPVNAISGDVKKKHYIDLELCVNCGTCKPACPVNA
                     IVSL"
BASE COUNT         1743 a          873 c          614 g         1802 t
ORIGIN      
        1 tatgtaaaga aaactgaaac acagattgca tataaggcgt ttcatcttga aaacgtggaa
       61 ggggagatga taaagtttca aaatctaaat aatatatagg atattctagt ttatccaacc
      121 actttttaat tgcttcttta ttaatatgtt gttcatcttt aataaaagct tctctttgaa
      181 ttttgtgtaa aacagatgta agctcactat cactatacat atccattgta attttgccct
      241 ctaagatatc tttatatttc tttttccccg caaaatataa agcagatcca ggaatgtttt
      301 ctaaataagc ataacaaact gggcaaaata aacattcttc gctattatca tttcttttac
      361 actttgaatc tatctctggt tgaataaaaa cattttgctt gattgcgctt tgaatctttt
      421 taacttctgc ttcaagtaaa tataaatatt cgttataaaa ttgccccatc tctagtaaag
      481 taacaatttc tcctttacct tctacataag gataaggatc atcaactgtt gctctattta
      541 gatctaaagt atattcgtga tttaaaaaag ctaaataaaa agatattttc tttaaatgag
      601 gattgttttt agtaactaac caataagtat acaaaccatc aaaaatatat ttgcctagtg
      661 cactagtagg attaaaaact tgatctacta aaaatttatc atatgtttca atttcatctt
      721 ctgtttcaag ttcactgcgt tcttttaatc ttaaaatgct ttcttctttc tcaaaaaaag
      781 gtttcttttc gtctataaat ttattagttg tcgttgcctt agcttctaaa acaataatct
      841 cattaatagt ttcttggtag ccatctattt ttgtgtaaaa agtattacct tcttgatcat
      901 gataaacaac ttctttttgc tctgctaaac tgcccgcttt aaggtttgtt tttaagattt
      961 tattagcata cttaattaac actctttccg tctccgcaaa ctgctcttgt tcatattctt
     1021 tcattgggtt ttctaacttg ttttcataag tattcttatc aatcatttcg tctaaatatt
     1081 tttgtaaatc aaataaactg ccatttacaa aactagcatt tcttttctct tcatatagtt
     1141 tatttaaact ataatacgac tcacaacgaa taagttcttt aaataatgtt ttacttatct
     1201 tcatcttttt acctctttaa agtattatat cataaaaaag ggcaaacgcc ctttttaata
     1261 tactttatct tttgtttttt tttatgatta aatgcaagat aaaatcaatt ataaaaacaa
     1321 aaataattaa aataattgtc caagcaaaaa ctcctttagt ttctaaataa gtattttgaa
     1381 tagcaatttg ataacctata gttcctttag tttgagcaat catttgcgct gttacaataa
     1441 ctttcaatcc caaaccaaaa gattgaagta acgatactaa aatctttggc tttgaaagtg
     1501 gaaaatagat atcttttata actaaataat ttaaattact tacagtttta acatcatcaa
     1561 gcaaatcttt atcaatagaa tttatactgc tataaatgcc ttcataaaga attggaaaga
     1621 ttaccaagaa agtaataaca taaggagtaa atctttgacc aaccattagt aaaaacaaga
     1681 cgataactgc tacaaccgga atacttttta gtaaagtaac tattggacta aagaaatgtt
     1741 tgaatccttc aaatcttaaa gcaagaagag aaaagacgaa agctaaaatt agagtcaaaa
     1801 tagttacaat taagatatct aaaaaggtcc ttaatataat taaatatgtt tctcccttag
     1861 tgaatagttc ccctattgtt ttaaaaacta aacttatttt tggtaaaaca atctcactat
     1921 ctacaagaat actaccaata aaccaaacaa agattacaaa caatatcccc aaagaataat
     1981 ataaatattt tttatcaatt ttattcatat ggggtataga aagcttgatc tggtaattta
     2041 ccacctacta aagcagcgtc ttcagttaaa agcaaattaa agaacttttc gatatcttct
     2101 ttaatatcta tagctttttt aaattcgata ttgcttagag gaattgaact agcaattaaa
     2161 tcttctttca tgctttctaa atattcatct ttttgataaa tgaaattggc atatccttga
     2221 ggatctctat ttaaatttgt aatattgttt tctactgcag taataaactt tgctattttc
     2281 tttgtgtttt tactttcact atttacaaaa atagcagctt gaggaactaa gtctaattcc
     2341 gttgttttat ataaaacatc agcaattgat aaatacttta aattagatac cttttttcgt
     2401 aaattagata atactggctc tgatgttaga agatattgat aatctccttt taagaaaaaa
     2461 gcgactccat catttattgc attataataa gtgatgtttg gatttacgtt ttcacttgca
     2521 agtgcacatc ttaaagtaat tcctggaatg ttagtttctc caaaagcagc caaagtttta
     2581 ccatctaaat cagctaatga tgtcaaaggt tcatcaatac ttacaatata agtatttaaa
     2641 aaaccaataa cagaatctag tttatagcta gagtttccag aattatacaa ttttgcccct
     2701 aaattaattg gagcaattat aatatctgct tcttttgaag taaaagcagt cactaataaa
     2761 ttaggtccag taacattatt atattcaata tcttcagtta aagtaccagc aacactaatt
     2821 agagggatac catttggaag tgttactttt aatttagcat cttcagtaca tcctgttaga
     2881 aaaacaaaac taaacaaaag taagaaaaag ctaactactc ttttcattat ttcttttctc
     2941 caaacacttc tttttcagaa tatgttgtat gtaatagttt gtgagctaat tcaccaccag
     3001 gtttacctaa atgagtttga tataaagaga ctacatttgg attttcatgg cttcttctaa
     3061 ttttattttt agaatcaatt gtatataaag cttgtgctct taatttgcaa acatcaattg
     3121 tttctaaatc tcttgcctta acaatcggtt gacctccacc attaatacaa ccgccttcac
     3181 atcccataaa ctctacaaaa atatacttat taggattttc tttaatcctt ctaaacattt
     3241 caggaaagtt tgctgctcca tggactacag caactttaaa agtttggtca tcaattacta
     3301 aatctgcttc tttaatagca actgatcctc taacttgagt aaaatcaatt gtatgtttat
     3361 cttctttttc taagaaagct ttaactgttc ttaaagcagc ctccataact ccaccagttg
     3421 taccaaaaat agctcctgct cctgtatatg taacaagggg tgattctggt ttataatctt
     3481 gcaagttatt aaagttaata ccaaatcttc taattaaacg tgcaaattca cgagttgtta
     3541 gaactgcatc aacatcttta acaccgcgat ctttcatttg ttcgcgatct gcttcatctt
     3601 tcttggcaat gcaaggcata aatgaaacaa ctttaattcg ctcttttgga acattaataa
     3661 tatctgcata aatatgttta attaaagccc cttgaatttg ttgaggagat tttgcagtcg
     3721 aaagatgagg taagtattct ggataatatc tttcaatata acgaatccat ccaggagaac
     3781 aagatgtaaa taaaggtaaa tgagcttctt ttccttgaag acggtcttga attctgccaa
     3841 ttaactctgt gccttcttcc ataattgtaa catcagctgc aaagttaaca tctgttattt
     3901 catcaaaccc cattttcttt aatgctcgat aaatcttacc ttcaacatta gttccaattg
     3961 gattgccaaa ctcttcacct aaagccgctc ttactgcagg tgcaacttga gcaattacat
     4021 aataattatc attattcaaa taatcaatta cgatatcttc atcactagtt cctgataaag
     4081 caccaaccgg acaagcttga atacatttcc cgcaatataa acaaccagca tcatcaatga
     4141 aatcattgtt tgcaggtgct acataagtat caaaacctct tctattgaaa tctaaaattc
     4201 ctaaacctgt ataatgatta caagcagata cacatctacc acataaaata catttagaag
     4261 tgtcaatagt taacgaattg gaaatattac gataactagt ttttttagaa aaagttggat
     4321 cttctcccat tgtattagga acattatatc ttcttaacaa ttccaacaat tcacagtttt
     4381 cttctctgtg gcaaacccaa cattcaaaat gatggttagc agcaagaagc tttaagttat
     4441 tcttaacagc atcatttact cttttagtat tagtatgaac tttcattcct tcccatactt
     4501 gaacagtaca tgaattcttt aacgtattta aaccctcaat ctcaacaaca caaagtctac
     4561 aagctgaagt ttcactaata tcttttaaga aacataaacg agggatataa atatcagcat
     4621 cattagcagc atctaatact gtataagtac taggtactgt catttcttgg ttattaatcc
     4681 atatttttac attactcata tttcttcacc tctataaact cacaattgcg ttaactggac
     4741 aagcaggttt gcatgttcca caattaacac aaagctcaag atcgatgtag tgttttttct
     4801 taacatcacc agaaatagca tttactggac aattacgtaa gcatttacca caaccaatac
     4861 attcatcagt aattaataat ttagtaatat tcttacaaac accagctgga catcttttat
     4921 taactacgtg ttctaaatat tcatcataaa acttatctag tgttgataaa actgggttag
     4981 gagctgattg acctaaagca cataaagaag tatctttaat cattaaagca ag
//
LOCUS       DAOSGS010000062         4986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_15004_length_4986_cov_4.243764, whole genome shotgun sequence.
ACCESSION   DAOSGS010000062 DAOSGS010000000
VERSION     DAOSGS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15004_length_4986_cov_4.243764"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..600)
                     /gene="rpmF"
                     /locus_tag="PLQ22_03170"
     CDS             complement(376..600)
                     /gene="rpmF"
                     /locus_tag="PLQ22_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOH58995.1"
                     /translation="MGAIAQQNRVSKTRKRKRRSHHALKNPALIACPECGEMMRSHVV
                     CPHCGYYDKKQVKEIKAKKAGAADGDEKAK"
     gene            complement(635..1018)
                     /locus_tag="PLQ22_03175"
     CDS             complement(635..1018)
                     /locus_tag="PLQ22_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58996.1"
                     /translation="MKWSLQQLKKFNNEVHEFSYEPNLKARTLETHDILDIKSLHVEG
                     TLKEISFNRFEVLFTIKGILVLEDAVTLEEIDYPIDLECEEICDAQMNADEDSYFIEK
                     NTLDLDELVWINIILHKPMRVSNSD"
     gene            complement(1117..1719)
                     /locus_tag="PLQ22_03180"
     CDS             complement(1117..1719)
                     /locus_tag="PLQ22_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58997.1"
                     /translation="MIRIRKEDDKRRRFLAYCIFLLILLVLLAFVIFYLMLFSDMYGG
                     KGLINKPGGSSGNSGGVVIGEAKSIDTIIDLEKLDTLEDKALVPVGYKDLSFSNGREI
                     VEEAEFTVAVSWKEKNDNYGITKEGRLSIISTAIIDGAVEYSYLVNVEIIAPETIILN
                     GDPVIVKFIVTLTEPPAEADYSSIVGKNIRILNAYNININ"
     gene            complement(1721..2314)
                     /gene="yihA"
                     /locus_tag="PLQ22_03185"
     CDS             complement(1721..2314)
                     /gene="yihA"
                     /locus_tag="PLQ22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOH58998.1"
                     /translation="MFEKCEYVTSIVSRKHSPNNDNRPEIVFLGRSNVGKSSLINLIT
                     NRKMLAKTSALPGKTVTINFYNIDDSFYLVDVPGYGYARRSKEDKERYGKYLEDYLTD
                     NPNIKVAFLLVDTKVGPTNDDLLMFEYLLYLELPIMVVATKSDKVNHSSLEKAKKQIR
                     EKLNLTDDSVIYTSSFKKIGRAEIIEVIQDEIEKEII"
     gene            complement(2363..2707)
                     /gene="rnpB"
                     /locus_tag="PLQ22_03190"
     ncRNA           complement(2363..2707)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ22_03190"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(2768..3106)
                     /locus_tag="PLQ22_03195"
     CDS             complement(2768..3106)
                     /locus_tag="PLQ22_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH58999.1"
                     /translation="MNCKKCGSPLGPNDYYCGVCGEKVEKEGEAVVTEPVMKNNTALT
                     LGIIGLILGSIVLGILAIVFSKKPNASNAKTAKILGIFAIVRAVIGVFYAIFCFTRND
                     RRYKGAIRSW"
     gene            complement(3157..3510)
                     /locus_tag="PLQ22_03200"
     CDS             complement(3157..3510)
                     /locus_tag="PLQ22_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH59000.1"
                     /translation="MNCKKCGNPVSPDAAFCGVCGEKIEKEVTVVNAPAGKQSWAAFV
                     LSIIGLSLCASGIVGLILSIIASSKAKKPNAALAKAAKILSIFGIIASAIFTLYWFIY
                     VIIIVIGIILGAATA"
     gene            complement(3714..4145)
                     /locus_tag="PLQ22_03205"
     CDS             complement(3714..4145)
                     /locus_tag="PLQ22_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOH59001.1"
                     /translation="MNCKKCGTEVREDALFCFNCGEKIEKDETFNIENKPVNKSKTAL
                     ICGIIGSVLPLIFPFVYLVLLIGIVIKVITGSAIGYELPFVIAISVIYLPTPLALGIV
                     AIIKSKDPNAVAKSAAKVFGVIAIVLWGLNIVLVISSLFSN"
     gene            complement(4164..4652)
                     /locus_tag="PLQ22_03210"
     CDS             complement(4164..4652)
                     /locus_tag="PLQ22_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH59002.1"
                     /translation="MKCKKCGHEMQETDLFCSNCGEPIAPEVEIVLKQVEEPKKKNKT
                     AFICGIIGIALPVAIYIIVVFFDLLFFMIDRVGMTAIMDVVLFAAPAVLILGTVAVKK
                     SKEEGAVYGKVALGFGIAAIVIGGTYSIVLFFNIIKLLYNLRDAYRNPEYFINLFKLF
                     FK"
BASE COUNT         1747 a          933 c          542 g         1764 t
ORIGIN      
        1 taacttatta acaactttat tccattcgtc attagggtac acgaaaaggc agcgttcgat
       61 ccctctgttt atgataagct tatctttcaa taaagatcga aactcagttg gtataaatat
      121 tctatttttg tcatctaaat tgaaatgata ttcacctata aacatagttt tctcctttct
      181 ccacttttca ccacttctac ccacttgaat tgtactacaa cttaataata aatgtcaagc
      241 atttttttaa acttttttga aaaaagttaa tattttttag ttatcaacgt ggaaaaagaa
      301 aaaagcactc aatttaaaga gtgcttttct tgtctttagg acatttcgtc ctttttttag
      361 ttttagatct agagcctatt tagctttttc gtctccatct gcagcgccag cttttttagc
      421 tttgatttct tttacttgtt ttttgtcata gtatccacaa tgaggacaca ctacatgtga
      481 tctcatcatt tcaccacatt caggacaagc aataagagct gggttcttta aagcatgatg
      541 gctacggcgc ttacgctttc tagtctttga aactcgattt tgttgagcaa ttgctcccat
      601 gttttttcac ctctatctct aattttaaag ttaattagtc actattacta actcgcattg
      661 gtttatgtaa gataatatta atccaaacga gttcatctaa atctaaagtg tttttctcaa
      721 taaagtaact atcttcatca gcattcattt gcgcatcgca aatttcttca cattccaaat
      781 caattggata atcgatttct tctagggtaa cagcatcttc taaaactaaa attcctttga
      841 ttgtaaaaag aacttcaaat ctattaaaag aaatttcctt aagcgtaccc tcaacatgca
      901 aagacttaat atctaaaata tcatgagtct ctaaagttct tgcttttaga tttggctcat
      961 aggaaaactc atgaacttca ttattgaatt tcttcaactg ttgtaaagac catttcatag
     1021 tatccctctt aactttaata cttataaagt atactatatt tttattcaat tgtcaataag
     1081 attaaactat taaaaaacta ctattaaata gtagttttaa ttaatgttaa tattatatgc
     1141 attcagaata cgaatatttt taccaacaat actcgaataa tcagcttctg caggtggctc
     1201 agttaaagtc acaataaact taacaataac aggatcacca tttaaaataa ttgtttcagg
     1261 cgctatgatt tctacattta ccaaataaga atattcgaca gctccatcta taatagcagt
     1321 actaataata cttaatcggc cttctttagt aataccataa ttatcatttt tttctttcca
     1381 agaaacagca actgtaaatt ctgcttcttc tacaatttcc ctaccattac taaagcttaa
     1441 gtctttataa ccaactggaa ctaatgcctt atcttctaat gtgtctaatt tctctaaatc
     1501 aataattgta tcaatagatt tagcttcacc aattacaact ccacctgaat taccactact
     1561 accacctggc ttattaatca agccttttcc tccatacata tcggagaata gcattaaata
     1621 aaatataaca aaagcaagaa gaactaataa aattagtaaa aaaatacaat aagctaaaaa
     1681 tcttcttctt ttgtcatctt cttttcgaat tctgatcata ttatataatc tccttttcta
     1741 tttcatcttg aattacttca ataatttctg cgcgaccaat tttcttaaat gatgatgtat
     1801 aaataacact atcatcagtt aagtttaatt tttctctaat ttgttttttg gctttttcta
     1861 gactgctatg attaacttta tcggattttg ttgctacaac cataatcggt agttctaaat
     1921 aaagtaaata ctcaaacatc aaaagatcat cattagttgg ccctacttta gtatcaacaa
     1981 gtaaaaaggc aactttaata ttaggattat cagttaaata atcttctaaa tattttccat
     2041 atctttcttt gtcttctttg cttcgtctag catagccata accaggtaca tctactaagt
     2101 aaaaactatc atcaatatta taaaaattga ttgtaacagt tttaccaggt aaagccgatg
     2161 ttttagcaag catctttcta ttcgtaatta agttaattaa actcgactta cctacgttac
     2221 ttcttcccaa aaaaacaatt tcgggtctat tatcattgtt tggactatgc tttctactta
     2281 caatacttgt aacatattca catttttcaa acatttttcc tccaaagtaa agtcccctgt
     2341 aacagggact tttaggtaat tattctataa gccttgttct gtacttcatt taagaagcgg
     2401 taatcattta tctctaataa attagcctta aatgaattga attatcattt aaagttcccc
     2461 taccaaattt gggtttctag cttgaggggt ttacccgttc cactttatca tttctaataa
     2521 attcgtcact gtcgcacttt atgattttaa ccatattcta aaacttaggt taaataatcg
     2581 ccgttactat ttattaaata gtaccctaac ttatgttttt gttaggctca aacactacag
     2641 catctcagct gtgctagcaa ggactttcct aacaattatt aaaataattg ctcgattacc
     2701 cgaattttta cctctaatag tatatcactg ttcctcttaa aaagcaagca aataattaat
     2761 aattatgcta ccaacttcta attgcgccct tatatcttct atcattcctg gtaaaacaaa
     2821 aaatagcata aaaaacacca attacagcac ggactatagc aaaaatccct aatattttag
     2881 cggtttttgc attactagca tttggctttt tagaaaatac aatagctaaa attcctaaaa
     2941 caattgatcc taaaattaac ccaataatac ctaaagttaa agcagtattg tttttcatta
     3001 caggttcagt tactacagct tcgccttcct tttcgacttt ttcaccgcaa acaccgcagt
     3061 aataatcatt aggccctaat gggctaccac attttttaca attcataatt tcctctcctt
     3121 tttaaaaata ctataaaata ctccaaataa ttacaattat gcagtagctg cacctaaaat
     3181 aataccaatt acaattataa taacataaat aaaccaatat agggtaaaga ttgcagacgc
     3241 aataataccg aatatactta aaattttagc tgctttcgca agtgctgcat ttggtttttt
     3301 agcttttgat gatgcaatga tgcttaggat taagccaact attcccgaag cgcataaact
     3361 aagacctata attgataaaa caaaagcagc ccaactttgt tttcctgctg gtgcattaac
     3421 aacagttacc tctttttcaa ttttttcacc gcacacgcca caaaaagcag catctggact
     3481 cactggattt ccacatttct tacaattcat aattttctct cctttcctgt aattaaatgt
     3541 tattatttct attatgacag agtatttata atgtaaatac tccgattaaa atagcaatca
     3601 cttaaataaa atttatatta attaataaaa gctataacgt ttaaagcaaa attaatctta
     3661 acaagaactt ttgcttcaca tttttaaaaa ttctaattcc ttctttttaa aaactaatta
     3721 ctaaacaaag aagaaattac taatacaata tttaatcccc ataatacaat tgctataacc
     3781 ccaaaaactt tagcagctga ttttgcaact gcatttgggt ctttagattt aattatcgca
     3841 actatcccca aagctaaagg cgttggcaag taaattactg aaatagcaat gacaaaaggt
     3901 aactcatatc caattgctga tccagttatt acttttatta caataccaat aagcaatact
     3961 aaataaacaa atggaaaaat taatggtaaa actgaaccta taattccgca aattaatgct
     4021 gttttgcttt tattaactgg tttattttca atattaaaag tttcatcttt ttctatcttt
     4081 tcaccacaat taaagcaaaa cagtgcatct tctctaactt cggtcccaca ctttttgcaa
     4141 ttcataattt tcacctaaat tatttattta aaaaataatt taaataaatt aataaaatat
     4201 tctggattac gatacgcatc gcgtaaatta tataataact taattatgtt aaaaaataaa
     4261 acaatcgagt aagttccacc aataactata gcagcaatgc caaaacctaa agctactttg
     4321 ccataaacag ctccttcttc ctttgatttt ttaaccgcta cagttcctaa tattaaaaca
     4381 gctggtgctg caaataaaac aacatccata atagctgtca ttccaacacg atctatcata
     4441 aagaaaagca agtcgaaaaa taccacaatt atgtaaatag ctactggcaa agcgattcct
     4501 ataattccac aaataaatgc agtcttgttt ttcttctttg gttcttctac ttgttttaaa
     4561 acaatttcca cttctggagc aataggttca ccacaattac tacaaaataa gtcagtttct
     4621 tgcatttcat gcccacattt tttacatttc ataatatcac tccatttcta aaaatatacc
     4681 ttcaaatatt taaactcagt tattaaataa gaatataata tgaaaatata taaactataa
     4741 atacacaagt taattttgca ccttctttaa taccaaaaag tgataaaata agaacaagat
     4801 tcctaaaacg aatattctta tactttattc gtaatattaa agcagttata ctaaatgata
     4861 atacaattag tccaagcgaa caaatgatag ttattaaaaa tattattgaa cagttttgaa
     4921 acatcccaaa caatttatcc aaaacacttc ctttgattac aaaatcaaga ggaaaaagaa
     4981 aagcta
//
LOCUS       DAOSGS010000063         4909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_15345_length_4909_cov_5.137824, whole genome shotgun sequence.
ACCESSION   DAOSGS010000063 DAOSGS010000000
VERSION     DAOSGS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15345_length_4909_cov_5.137824"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLQ22_03215"
     CDS             <1..474
                     /locus_tag="PLQ22_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOH59003.1"
                     /translation="FLMYIGASPAGTGGGIKTTTFFTAAKYIGSFARGTRPITHNREI
                     SKTSVSKAFVLIVLSIVYLAMIVLFISVIEQLNTASEDADLIKIMFETLSAFSTVGNS
                     EGITPTLHWVSKILISATMLIGRLGPITIISLLNRRWNKEGTEPVSYLEEKIIIG"
     gene            486..1148
                     /locus_tag="PLQ22_03220"
     CDS             486..1148
                     /locus_tag="PLQ22_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOH59004.1"
                     /translation="MKKTIAVLGLGRFGLGLVEELNKLNVDIIAIDINGASVQKAGEF
                     VENAFICDSTNEDALREIGIENVDHAVVAFGSNFQASLITVITLKEMGVEKITVRIDD
                     EKYASVMQRIGATDIIIPQKIAGIQLANKLVSDSFTNYFNLSEEYVVVQLSVNMTIKP
                     VKLKTLNARNVFGINLLLIKRENKHFSPTGDDSILPGDEIFVLGTKKNIHKFDQHINE
                     RE"
     gene            1150..1596
                     /locus_tag="PLQ22_03225"
     CDS             1150..1596
                     /locus_tag="PLQ22_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59005.1"
                     /translation="MKKLFLIFVLLLGVFLLTSCSCSSDKKTYTCYEYTESVKYKNTS
                     KFVTLDMFASTYKEIKLYLNYQDHTFKRVGKFRDEVADEIYTGTFEEGEGKLTFSYDS
                     DIKKHFENEWGKEVYIFVGKELHMTLERGTTAPSGTVWGPIVMKYK"
     gene            complement(1613..2380)
                     /locus_tag="PLQ22_03230"
     CDS             complement(1613..2380)
                     /locus_tag="PLQ22_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH59006.1"
                     /translation="MHQTLKIILISVSIALLVALIIRALIKNKKEKEKPSLGKRRNQL
                     RIMILIAIFSSFSVIAYQLNFSLPFFPPFLKIHFSNLPILIGGFTLGPMSGIGMLLLR
                     FLFGIITTKSAYVGEITDLIIGFASVIPAAIIYKKYRTKKGAGISLIVSTIVWVVTAV
                     FINYIFVIPAYIKLYFNGDVETFVQTISVIPGVNENNYMGRYLLFATLPFNLLLSITI
                     NIITFLVYKRISPLVHEFTEKEDLEKEETEEQKNTLE"
     regulatory      complement(2440..2563)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2590..3825)
                     /locus_tag="PLQ22_03235"
     CDS             complement(2590..3825)
                     /locus_tag="PLQ22_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH59007.1"
                     /translation="MKKMWAIAKKDLDKVFKFPGTLFTTVLLPGLIFFLVYTSISVII
                     NRQIDKEQEKEKIVYVYEAPTGFIEFQTYASQNKLLDSSLTFVYSDYEYTRTELEAKM
                     KDDEHYIIIVFDEDFYNKAVAVTNETPAEDLPNVKILYNDQNEFLGPTAQALYYFMAA
                     YKNFVVNQELSSVGKTTEIFTYKMESILDEVTQNTMILAMMLPMMLMIIIFSGTLGIG
                     VDAIAGEKERGTLASLLMMPVRREQIIIGKMLSTSILSLLVAASSFIGMLSSTLVGGE
                     NMFGIDLTAELGYGFGDYMMLLLILIVIAFTVSILVLICSTLGKNPQQAASYAMPLYI
                     VCIMLGIAASSTDLGLGFGKYFVPIFNLNVLLSDLISFKLTVGNVLATVGSSIAFIGV
                     IVVLLVRAFRSERILFDKQ"
     gene            complement(3822..4598)
                     /locus_tag="PLQ22_03240"
     CDS             complement(3822..4598)
                     /locus_tag="PLQ22_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH59008.1"
                     /translation="MKEVIVVENLVKTFKLSPKQMKTLKTKDKKKIAVNDVSFTTYEG
                     EIFGLVGPNGAGKTTALRCISTLIKPDSGKITIKRIDISEELKIKKKLAFLTSELKLE
                     DKFSPNYLFDYYSNLHDVPKDIAAARKQDLFTRFEVNEFSDTKIGDLSTGMKQKVSVA
                     ISLVHQPDIIVFDEPTNGLDVYTAKIVTDYLLELKKQGKSIIISTHIMSLVEKLCDRV
                     GLIFNGKMVICDTLDKVLDSVPSRDLEEVFIKLYQEEGAQ"
     gene            complement(4595..>4909)
                     /locus_tag="PLQ22_03245"
     CDS             complement(4595..>4909)
                     /locus_tag="PLQ22_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOH59009.1"
                     /translation="FSKREGTPAFKYESNVLPKEAKERLLKLNEEINQGFLKGNKRFE
                     KEIVKVLVDGFSDKDESVLSGYTEHNKLVNFNGDKSLIGEIVDVKITKAYTWHLKGEL
                     QK"
BASE COUNT         1742 a          815 c          657 g         1695 t
ORIGIN      
        1 ttcttaatgt acataggagc atccccagcc ggtactggtg gaggtattaa gacaacgact
       61 ttctttactg ctgcaaaata tattggttct ttcgcaagag gaacaagacc aattactcat
      121 aatcgagaaa tttcaaaaac ttcagtttct aaagcttttg tattaattgt actttcaatt
      181 gtttatctag caatgattgt tttgtttatt tcagttattg aacaattaaa tactgctagt
      241 gaagatgccg atttaattaa aattatgttt gaaacactgt cagctttttc aaccgtagga
      301 aactcagaag gtattacacc tacattacat tgggttagta aaatattaat ttcagctaca
      361 atgcttatcg gtcgtttagg accaatcaca attatcagtt tattaaatag acgctggaat
      421 aaagaaggta cagaacctgt atcttattta gaagaaaaaa tcattattgg ttaggagata
      481 taaaaatgaa aaaaactatt gctgttttag gcttaggtcg ctttggctta ggtcttgtag
      541 aagaattaaa caaactaaat gttgatatta ttgcaattga tatcaatggt gctagtgtac
      601 aaaaagctgg agagtttgta gaaaatgcct ttatttgcga ctctacaaac gaagatgctt
      661 taagagaaat tggaattgaa aatgttgatc atgctgttgt agcttttggc tcaaactttc
      721 aagcaagcct aattactgtc attaccctaa aagaaatggg tgttgaaaaa attaccgtta
      781 gaattgatga tgaaaaatat gcttctgtta tgcaaagaat aggcgcaact gacatcatta
      841 ttcctcaaaa aattgctggt attcaacttg ccaacaaatt agtatctgat agctttacaa
      901 attacttcaa cttatctgaa gagtatgttg ttgtccaact atcagttaat atgacaatta
      961 aaccagttaa actaaaaaca ttaaatgcta gaaatgtttt cggaattaac ttacttttaa
     1021 ttaaaagaga aaacaaacat ttttcaccta ctggggatga ttcaattcta ccaggcgatg
     1081 aaatatttgt tttaggaaca aagaaaaaca ttcataaatt cgatcaacac ataaatgaga
     1141 gggaataata tgaagaaact atttttaatc tttgttttat tgttaggtgt atttctacta
     1201 acatcttgtt cttgttcaag cgacaaaaag acttatactt gttatgaata tacagaatca
     1261 gttaaataca aaaatactag caaatttgtg actttagata tgtttgcaag cacatataag
     1321 gaaattaagt tatacttaaa ttatcaagac cacactttta aaagagtagg taaatttcga
     1381 gatgaagttg cagatgaaat ctatactgga acttttgaag aaggagaagg taaattaacc
     1441 tttagctacg atagtgatat aaaaaaacat tttgaaaacg aatggggtaa agaagtatat
     1501 atctttgtag gtaaagagtt gcacatgaca cttgaaagag gcacaacagc accatctggt
     1561 actgtttggg gccctattgt aatgaaatat aaataaataa aaaacacatt agttactcta
     1621 atgtgttttt ttgttcttca gtttcctctt tttccaaatc ttctttttct gtaaactcat
     1681 gtactaatgg tgaaatcctc ttataaacta agaaagtaat aatgtttatc gttatcgata
     1741 aaagtaaatt aaatggtaat gtggcaaaaa gtaaatatct acccatataa ttattttcat
     1801 tcacaccagg aattacacta atcgtttgaa caaatgtctc tacatcacca ttaaaatata
     1861 atttaatata tgctggaata acaaaaatat aattaataaa aacagctgtt actacccaaa
     1921 caatagtact tacgattaaa gagattccag ctcctttttt tgttctatat tttttataaa
     1981 taattgcagc cggaataact gaagcaaagc caataattaa atctgttatc tcccctacgt
     2041 atgctgactt agttgttata attccaaata aaaatcttaa aagcaacatt ccaatacctg
     2101 acattggacc taaagtaaac ccacctatta aaattggcaa gttagaaaaa tgaattttta
     2161 aaaatggggg aaagaaaggt aaagaaaaat taagctgata tgctattacc gaaaacgaac
     2221 taaaaatagc gatcaaaatc attattctta gttgatttct tcttttaccc aaacttggtt
     2281 tttctttttc ttttttgttt ttaatcaaag ctctaataat taatgccact agtaaagcaa
     2341 ttgatacact gattaaaata attttcagag tctgatgcat gttacctcca ataaaaaact
     2401 cgtgaaaatc ctcacgagca aaaaatcaat ctaactacat agactcttct tttatctaga
     2461 ctattactat cggttttgga attgcaccaa atcgtgtctc taaattagaa actcgtggac
     2521 ttttaccacc ggtcgagaat tgcaccctac cctgaagatt tattcatata gattattata
     2581 cattattttt tattgtttgt caaacaggat tcgttcactt ctaaacgctc ttacaagtaa
     2641 cactacaatt acaccaataa aggctattga tgaaccgaca gtcgcaagca catttccaac
     2701 tgttaattta aacgaaatca aatcacttaa taaaacattt aaattaaaga taggtacaaa
     2761 atatttccca aatcctaaac ctaaatctgt tgatgaagca gcaattccta acataataca
     2821 aacgatatat aaaggcattg cataacttgc agcttgttgt ggattctttc ctaatgttga
     2881 acaaattaaa actaaaattg atactgtaaa agcaataaca attaagataa gtagtaacat
     2941 catataatca ccaaaaccat atcctagctc tgctgttaaa tcaataccaa acatattttc
     3001 gccaccaact aaagtacttg atagcatacc aataaatgaa gatgctgcta caagtaaaga
     3061 taaaatacta gttgataaca tcttacctat aataatttgt tccctacgaa ctggcatcat
     3121 taagagtgaa gctagcgttc ctctttcttt ttctcctgct attgcatcaa caccaatacc
     3181 caatgtccca gagaaaataa taatcattaa catcattggt aacatcatag caagtatcat
     3241 tgtgttttga gtaacttcat ctaaaatact ttccatttta taagtaaaga tctctgttgt
     3301 cttaccgaca ctacttaatt cttgattaac tacaaagttt ttataagcag ccataaaata
     3361 atacaaagct tgtgctgttg gtcctaaaaa ctcattttga tcattataca atatcttaac
     3421 atttggtaag tcttcagctg gtgtttcatt agtaacagct accgccttat tataaaaatc
     3481 ttcatcaaaa acaataatta tataatgttc atcatctttc attttagcct ctagctcagt
     3541 tcttgtgtat tcatagtcag aataaacaaa tgtaagcgaa gaatctaata acttgttttg
     3601 tgatgcatat gtttgaaatt caataaaacc tgttggtgct tcataaacat aaactatttt
     3661 ctctttttct tgttctttat caatttgtct attaataatt acagaaattg aagtatacac
     3721 taagaagaaa ataagtccag gtagtaaaac agtagtaaat aaagttccag gaaatttaaa
     3781 aactttatct aagtcttttt tagctattgc ccacatcttt ttcattgtgc gccctcctct
     3841 tgatataact taataaatac ttcttctaaa tctcgtgaag gaacagaatc taaaacttta
     3901 tctagtgtat cgcaaataac catttttcca ttaaaaatta atccaacacg atcacataac
     3961 ttttctacaa ggctcataat atgtgtactt ataattattg acttgccttg tttttttaat
     4021 tccaataaat aatcagttac aatcttcgca gtgtaaacat ctaatccatt agttggttca
     4081 tcaaatacaa tgatatcagg ttgatgaact aaactaatag caactgaaac tttttgtttc
     4141 atacccgtag ataaatcacc tattttagta tcgctaaatt cattaacttc aaaacgagta
     4201 aataaatctt gctttcttgc agctgctata tctttaggta catcatgtaa gttagaataa
     4261 taatcaaata aatagtttgg tgaaaacttg tcttctaact tcaattcact agttaaaaat
     4321 gctagtttct ttttaatttt gagttcttcg gaaatatcaa ttctcttaat tgtgatttta
     4381 ccagaatctg gtttaattaa agttgagata caacgaagag ctgtcgtttt acctgcccca
     4441 ttaggaccaa ctaaaccaaa gatttcccct tcgtaagtag taaaacttac atcgttaact
     4501 gctatttttt tcttatcttt agtttttaaa gtcttcattt gttttggtga aagtttaaac
     4561 gtctttacca aattctcaac aacaattact tctttcattt ttgcaattct cctttcaaat
     4621 gccatgtata tgctttagta atcttaacat ctacaatctc tcctattaaa gatttatctc
     4681 cgttaaaatt tactaattta ttgtgttctg tatacccaga taacactgat tcatctttat
     4741 cactaaatcc atcaaccaat acttttacaa tctctttttc aaatctctta tttcctttta
     4801 aaaatccttg gttgatttcc tcatttaact taagtaatct ttctttagcc tcttttggta
     4861 aaacattact ttcatattta aaagctggag tcccttctct tttagaaaa
//
LOCUS       DAOSGS010000064         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_15807_length_4803_cov_2.973673, whole genome shotgun sequence.
ACCESSION   DAOSGS010000064 DAOSGS010000000
VERSION     DAOSGS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_4803_cov_2.973673"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLQ22_03250"
     CDS             <1..599
                     /locus_tag="PLQ22_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59010.1"
                     /translation="FSEKLDVEEYINEYKKFRANSYLSGFEKSVDELSIILCKTAISH
                     RYPLYEELLAKVEEEKDYSAVEKFFYYQSWFEYYIDEANIGKAEYIHQEMEKLYPRLP
                     VQLQKHLSKEIGVNRLTLNVYKQEHIPQTEIILQTLLQQRITKISFIQVMYLLGIISY
                     NQKDYITANYRFSYVFNNGKSTKWKEAAKAYLDVLHTV"
     gene            complement(1177..1593)
                     /locus_tag="PLQ22_03255"
     CDS             complement(1177..1593)
                     /locus_tag="PLQ22_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOH59011.1"
                     /translation="MKHYRKVLVFNTNQRRSFINITKEVEEAVKESKIQNGLVLCNAM
                     HITASVFINDDESGLHKDFEIWLEKLAPEKPYNQYYHNTYEDNADAHLKRTVFGREVV
                     VAITDGKLDFGPWEQIFYGEFDGKRPKRVLIKIIGE"
     gene            complement(1594..2517)
                     /locus_tag="PLQ22_03260"
     CDS             complement(1594..2517)
                     /locus_tag="PLQ22_03260"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOH59012.1"
                     /translation="MQNQAKIKNYLLNSNNFDLTVFSNDVKSPKAILIIVHGMAEHID
                     RYQGFFDYLREHQILAIGYNQRGHYLGVEKEEDYGFLREKDGFGLLISDLNNFYLDVK
                     AKYPNLPIFIFGHSMGSFITQRFIQKYDYKLNGVILSGTGKNPNLILNLGHFLTKLII
                     FFKGPRYRSKFIHNLSFGSYTKKYKDKRTDLDWLSRDHEIVDKYIKDKWCGGVFSTAF
                     FRDFYHLMSRVNKDNRLLQPLFPIIMLSGDMDPVGNYGKGVTKLYNIYKKQNIDVKMK
                     LYKDARHEILNDFCKEEVRSDILNFIEERIK"
     gene            complement(2496..3680)
                     /locus_tag="PLQ22_03265"
     CDS             complement(2496..3680)
                     /locus_tag="PLQ22_03265"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOH59013.1"
                     /translation="MKFKRIFLIVIDSVGVGYDNKAHLYGDEGANTLKHLSYSKKDFS
                     IPTLRKLGIGSITDVNRANCKNPKGVYGKIEEESVGKDTLTGHWEMMGIKSDKPFPSF
                     TENGFPKELIDLLEKESGYKMLGNIAASGTEIIKDLGEKSINEKAMIVYTSADSVLQI
                     AAHVETIPLKELYRTCEIARRITIENDNWKVGRIIARPFIGADKNSFVRTADRHDYVL
                     APPRNTVLDALKGSKFDVIAVGKINDIFNGEGITKAYKTKSNQEGMEMTIHHLKENFT
                     GLVFTNLVDFDSQYGHRRDPIGYAKSLEEFDRQLKVLINNLNDDDLVIVTADHGNDPT
                     FKGTDHTREYIPLIVYANNIIPQDLNTLFSFASIGATIAENFGVEAPPIGRSFLCKIK
                     QK"
     gene            complement(3681..4586)
                     /locus_tag="PLQ22_03270"
     CDS             complement(3681..4586)
                     /locus_tag="PLQ22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOH59014.1"
                     /translation="MNLKDALVEFRYYLRGDKNVSKATFAAYEKDVEDYLEFLAKYRE
                     RKDVRDISANDIRKHLESLKRKNLEASSVARKLTAIKSFHTFLMIEKYTNYNYASVID
                     SPKKQKKLPVVLSEKEIDILLNSIDQKTEEGIRNKTMILLAYSSGLRVTELCTLKFSN
                     LRLEQRLLNITGKGKKMRIVPVSIEAALAVDNYLKNARPFFDKGKDKESVFLTREGTT
                     INRVTFYEILKEACEKCGIRKKVSPHTLRHSFATHLLNKGLDLRMIQELLGHSDISTT
                     EIYTSIGQEQLRKTLAHHPRARKDK"
BASE COUNT         1567 a          743 c          712 g         1781 t
ORIGIN      
        1 tcttttcaga aaagttggat gttgaagaat atattaacga atataaaaaa tttagagcta
       61 atagttattt aagcggcttt gaaaaaagtg tagatgaact atcaataatt ctttgtaaga
      121 cagctatttc tcacaggtat ccgctatatg aagagctatt agcaaaagta gaagaagaaa
      181 aagattatag tgccgtagaa aagtttttct attatcaatc atggtttgaa tattatatag
      241 atgaagctaa tataggaaaa gctgagtata ttcatcaaga gatggaaaag ctttatccaa
      301 gacttcctgt tcaattacag aaacatttat caaaggaaat tggtgtcaat cgtttaacat
      361 taaatgtata taaacaagaa catattccac aaacagagat aatcttgcaa actctcttgc
      421 aacaaagaat cacaaaaatc tcatttattc aagtaatgta tttattggga ataatatctt
      481 ataatcaaaa agactatata actgcaaact atagattcag ttatgtattc aataatggta
      541 agagcacaaa gtggaaagaa gcagcaaaag cttatttaga tgtattgcat actgtttaaa
      601 accttaattc tgggccgatt atcttttaaa gatgaagaat tctaaattaa actctttgta
      661 aaaggcctat ataaataagg ttttaaaaga aaaatattaa aataaaataa aaataaaatg
      721 tagacaatta agtctacatt ttttatttgg ttatttatat ttcaattaaa aagtttaagg
      781 ataattaata tgatcgaaaa gtttggaaat agaaactaaa tataagagta gcctataaac
      841 tatgagcagt agtaatatta ataaacctct taaataaagg ctattatttc ttatagttat
      901 aactgccgat aacatacatt atgttaaagt aaatatatat aggatgatgc cttttataag
      961 gaggcagtgt gtataagtag ataatgtgta aatctctata tctatgatta taaggctttt
     1021 tgatataaaa tacaaggttt ttatggataa aaacggcata gaaatagggc aaaaaagagc
     1081 agtttttagg gtaaaaacaa acaaaaaagg cttataaaaa gccttatttt gaataaataa
     1141 tcatattcat aatttgtttt tgagtatgaa taagggttat tctcctatta ttttgattaa
     1201 tactcttttt ggtctttttc cgtcaaattc gccatagaat atttgttccc aagggccaaa
     1261 atctagtttt ccatctgtta tagctacaac tacttctctt ccaaaaacag ttctttttag
     1321 gtgagcatct gcattgtctt cataagtatt atgataatat tggttgtaag gtttttctgg
     1381 agctagtttt tctagccaaa tttcaaaatc tttgtgtaag ccagattcat catcattgat
     1441 aaatactgat gctgtaatat gcattgcatt acataaaact aaaccatttt ggattttgct
     1501 ttcttttact gcttcttcta cctctttagt aatgtttatg aagcttcttc tttgatttgt
     1561 attgaaaact aaaactttac gatagtgttt catttattta atcctttctt caataaagtt
     1621 taagatatca gatcttactt cttctttaca aaaatcgttt aagatttcat gacgagcatc
     1681 tttgtataat ttcatcttta catcaatatt ttgctttttg tatatattgt atagtttagt
     1741 tacaccttta ccataattac ctactggatc catatcacct gagagcatta ttatagggaa
     1801 aaggggctgt aggagacgat tatctttatt tacccttgac attagatgat aaaagtctct
     1861 aaagaaggct gtagaaaaga ctcctccaca ccatttatcc ttaatatact tatctactat
     1921 ttcatggtct ctacttaacc agtcaaggtc agttctctta tctttgtatt ttttagtgta
     1981 tgagccaaag gaaagattat ggataaattt ggatctatat cttggccctt taaagaaaat
     2041 aattagttta gtaaggaaat gaccaagatt taagattaga ttagggtttt tacctgttcc
     2101 agatagaatc actccattaa gcttataatc atatttttgg ataaatctct gtgtgataaa
     2161 agatcccatt gagtggccaa aaatgaatat tggtaagtta ggatatttag ctttaacatc
     2221 taaatagaaa ttatttaagt cacttattag taaaccaaaa ccatcttttt ctcttaaaaa
     2281 tccgtaatct tcttcttttt ctacccctaa atagtgtcct ctttgattat agccaatggc
     2341 taggatttgg tgttctctta gataatcaaa aaatccttga tagcggtcaa tatgttctgc
     2401 cataccatgg acaattatta gtatagcttt tggacttttt acatcatttg agaaaacagt
     2461 taagtcaaaa ttattggaat taagtaaata attctttatt tttgcttgat tttgcataaa
     2521 aagctccttc caataggtgg tgcttcaaca ccaaagtttt ctgctattgt cgctccaatt
     2581 gaggcaaaac taaataaagt gtttaaatct tgaggaatga tattattcgc atagacgatt
     2641 aatggtatat attctcttgt atggtctgtt cctttaaatg taggatcatt accatgatcg
     2701 gcagttacga ttactaaatc atcatcattt aggttattaa tcaagacttt taactgccta
     2761 tcaaattctt ctaaagactt agcataaccg attgggtctc ttctatgacc atactgtgaa
     2821 tcaaagtcta caaggttagt aaagactaaa ccagtgaagt tttcttttaa atggtgaata
     2881 gtcatttcca taccttcttg gttagatttc gttttatagg ctttagtaat gccttctcca
     2941 ttaaagatat catttatctt tccgacggct ataacgtcaa atttagagcc ttttaaggcg
     3001 tctaggactg tatttctagg tggagccaag acgtaatcat gacgatctgc agtccttaca
     3061 aaactgtttt tatcagctcc aataaatggt ctagcaatga ttctacctac tttccagtta
     3121 tcattttcaa ttgtaattct tcttgcaatc tcacatgtac ggtataattc ttttaaggga
     3181 attgtctcta catgagctgc aatttgtaag acagaatcag ctgaagtata gacaatcatt
     3241 gctttttcat taatcgattt ttcacctaaa tctttaataa tttctgttcc tgatgcagca
     3301 atattaccta acattttata acctgattct ttttcaagta aatcaattag ttctttagga
     3361 aagccatttt cggtaaatga aggaaaaggt ttatctgact ttattcccat catttcccag
     3421 tgaccagtta gggtatcttt tccaacactt tcttcttcta ttttgccata tacgccttta
     3481 ggattcttac aattagcacg atttacatca gtaattgaac caattcctag ttttcttaat
     3541 gttgggatgg agaagtcttt tttgctataa cttaagtgtt ttaaggtatt tgcgccctca
     3601 tctccatata aatgggcttt attatcataa ccaacaccaa ctgagtcaat tacgattaaa
     3661 aagattcgtt taaatttcat ttatttatcc tttctagctc gtggatgatg agctaatgtt
     3721 tttcttaatt gttcttggcc aatgcttgta tagatttcgg ttgtggaaat gtcggagtgg
     3781 cctaatagtt cttggatcat ccttaagtct aaacctttat ttaaaaggtg ggttgcaaag
     3841 gaatgtctta gagtatgagg acttaccttt ttcctaattc cacatttttc acaagcttct
     3901 tttaaaatct cataaaaagt aactcgattt atcgttgttc cttctctagt aagaaagaca
     3961 gattctttat cttttccttt atcgaaaaat ggtcttgcat tttttaagta attatcaacc
     4021 gctaaagcag cttctatact aacaggaaca attctcattt tctttccttt accagtaata
     4081 ttaagaagtc tttgttctaa gcgaagatta gaaaatttca aagtgcaaag ttctgtaaca
     4141 cgaaggccag atgaataagc taaaagaatc attgtcttat ttctaattcc ttcttctgtt
     4201 ttttgatcaa tgctattaag taaaatatca atttcttttt ctgataagac tacaggtagc
     4261 tttttttgct ttttagggct atcgattaca cttgcataat tgtaatttgt gtatttttca
     4321 atcatcaaaa acgtatgaaa tgattttatg gcagttagtt ttcttgcaac tgatgaagct
     4381 tctaggtttt tgcgttttaa gctttctaag tgcttacgaa tatcgtttgc tgagatgtca
     4441 cggacatctt ttcgttctcg atatttagct aaaaactcta aatagtcttc tacatctttt
     4501 tcataagctg caaaagttgc tttagaaaca tttttatcgc ctcttaaata gtaacgaaac
     4561 tcaactaagg catcttttaa gttcattata ttttcctcca ttttcctcta aaaacaggca
     4621 aaaaacgacc ctttctgtga ggagattatc ttttttttgt gaagaattcc ccactaatcg
     4681 tcccacagag cgcctcaaat taggccattt ttaatctaaa aattcaacta aaatactatt
     4741 gattgtataa agatattttt cggtaatttt gaatttattt tccttaaaaa ttattttttc
     4801 ttc
//
LOCUS       DAOSGS010000065         4742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_16113_length_4742_cov_4.187327, whole genome shotgun sequence.
ACCESSION   DAOSGS010000065 DAOSGS010000000
VERSION     DAOSGS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16113_length_4742_cov_4.187327"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..459)
                     /locus_tag="PLQ22_03275"
     CDS             complement(91..459)
                     /locus_tag="PLQ22_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOH59015.1"
                     /translation="MNKRDTKQKQIVYEALLEHKDHPTSLEIYEELKNKGARIGKTTV
                     YRILNNLVQEDKLRRINTFDNVSHYDYVLDDHIHFVCEKCNKIVDLENTNDLKDALEE
                     MDVSHNNLLIYGICSDCKGG"
     gene            complement(465..1142)
                     /locus_tag="PLQ22_03280"
     CDS             complement(465..1142)
                     /locus_tag="PLQ22_03280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH59016.1"
                     /translation="MEERLETRIPLIGDKAPSFKAVTTQGEINFPEDYKGKWVILFSH
                     PADFTPVCTTEFITFGAMMDEFKKLNTELVGVSIDSLHSHIAWLRAAGQIEWKNYKNV
                     EIKFPLIDDVKMEVSKKYGMLQPGFSDTQAVRAVFIIDPKGIVRLIMYYPSSTGRNFD
                     EIKRVIIAMQKAEKEGIATPADWRPGDDVIVPPPGTCGAAKERLQDKAEDMYCLDWYL
                     CFKKDKK"
     gene            complement(1263..3674)
                     /locus_tag="PLQ22_03285"
     CDS             complement(1263..3674)
                     /locus_tag="PLQ22_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH59017.1"
                     /translation="MKKFAKIYNIIISIIFIAVLVFSIIDVNEVEYKTELNKDNIYTH
                     LDNLESRPIIDKEAKEAARDYIVNTIEGYGFVNQNVLGYSYLIQKTSSEKVDKNRETL
                     ETIVEGLDPQDSVAYHDFVLENIVVYLPANGAEKTNDAVMFMAHYDSVPMGPGSADDG
                     SMVAVMLEAIRYYKIKLTKNEIVTNNDLVFVFTDGEEYGCLGSAKYVSDFTGFNNLVQ
                     RTKLGINLESRGSSGTAIMFETSQKNSKLIKLYKDLDQTPFVSSISNMVYQMMNNFTD
                     FTSFASEIKGYNIANIGGGEHYHTQNDNRENLPKSYISQAANIVTSTVEATKDYNLNE
                     LYLTDDDVVYFSYLNLGTVSYSKGFAYALNSLLLLLFIALIIITIYRKQTNYKDTIKS
                     IAVIVGSLFFVVLAIFVLYYVSAFIAALFGTVNFHRISDITYNNNLITILLILMTALF
                     ASLAIAKGATWFKLERKSLRKGLTYVITFIAVLINIFLPMVSYLFAYLAMFMIIRELL
                     EELFPKLDSFNIRFSLTILTLTIPLAVPVIFLAVAALGIKSAYLVAVIISIYALVFAV
                     SNLDKFKYLDTFYFMNRRRKKSLVLDRTRGVYCTIMKVVIVLIITLAIPVHLSDNVSS
                     SQKYAMYPYDDAVVYLKTEEGLRAEIRDLDAYQSLNKELDGFRYRDGVYKKSLENKDF
                     TKLADVKLVGNQLQITKSSETTYVEIIVSNNKNITGYFYEIDGITFARRFETVEEHFV
                     LRFSENTVVTFKILEEAALDANLTSYELAVNHQELMTFEEKLLLADAKYKNARFNLIY
                     KKEIR"
     gene            complement(3703..4575)
                     /locus_tag="PLQ22_03290"
     CDS             complement(3703..4575)
                     /locus_tag="PLQ22_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH59018.1"
                     /translation="MRKFVMFVDSGCDLPVKYIEKYDFKTVALTVTIEEEVITEYFDE
                     AKKRKMWDAIRDKKNLKTSLVNVSEFIEGFSPYLEQGYDIFYMAISSGLSGTFNAARM
                     AKLELEDMYPERTIKIVDSLSGSLAEGLLAIYAKEMLDEGMDWDLVEEKMNEMAVHMH
                     HFFVIDDLWYIRRGGRLSGTKAFIASLLKVKPVCTANLKGEVTVVERHRGRKKALARI
                     LEIVKEKIIDPINQIIGIGHGDDEKGAKETKHALEKELGVKNTILTSFGPIIGIHGGP
                     GNIALFFLGKKREA"
     gene            complement(4605..4678)
                     /locus_tag="PLQ22_03295"
     tRNA            complement(4605..4678)
                     /locus_tag="PLQ22_03295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4644..4646),aa:Gly,seq:ccc)
BASE COUNT         1607 a          889 c          546 g         1700 t
ORIGIN      
        1 tgtcaagcgg agagtgctaa tttatcaaaa gaaaacgttt ttattttatt cgtacataaa
       61 aaagcttgtt tttaaaaaca agcttttctt ttatccccct ttacagtcag aacaaatacc
      121 ataaataagt aagttgttat gactaacatc catttcttct aaggcatctt ttaagtcatt
      181 tgtgttttct aaatcaacaa tcttattgca tttctcgcaa acaaaatgga tatgatcgtc
      241 taaaacataa tcgtaatgag atacattatc aaatgtattg attctcctaa gtttatcttc
      301 ttgaacaaga ttattaagga ttctataaac agtagtcttt cctattcttg cccccttatt
      361 tttaagttcc tcataaatct ctaaacttgt aggatgatct ttatgttcta aaagagcctc
      421 ataaacaatt tgtttttgtt tagtatcacg tttattcaaa caaattactt cttatctttt
      481 ttaaaacaca aataccaatc tagacaatac atgtcttctg ccttatcttg taatctttct
      541 tttgcagctc cacatgttcc tggtggcgga acaattacat catctcccgg tctccagtca
      601 gctggtgttg caataccttc tttttcagct ttttgcattg caataattac acgcttaatt
      661 tcatcaaagt ttcttcctgt tgatgaagga tagtacataa ttaatcttac aataccttta
      721 ggatcaataa tgaataccgc tcttacagct tgtgtatcag aaaatcctgg ttgaagcatt
      781 ccgtactttt tagatacttc catctttaca tcatcaatta atgggaactt aatctctaca
      841 ttcttatagt ttttccattc gatttgccct gcagcacgaa gccatgcaat gtgtgaatga
      901 agtgagtcaa tagaaactcc aactaattct gtattaagtt ttttaaattc gtccatcatc
      961 gccccaaaag taataaactc tgttgtacaa acaggcgtaa aatcagctgg atgcgaaaat
     1021 aaaataaccc atttcccttt gtaatcttct ggaaagttaa tttccccttg tgtagtaaca
     1081 gccttaaaac taggcgcttt gtcaccaatt aatggtattc ttgtttctaa tctttcttcc
     1141 atttataacc tcctatattt ttccttaatg gaactcattt tcaatagtat tataacaatc
     1201 tttattactt actgcaactt taatgctcaa ataaaacaaa aatagatgta ctttaagtac
     1261 atctatctta tttctttttt atatattaag ttaaatcttg catttttata tttagcatca
     1321 gctagcaata gtttttcttc aaaagtcatc aactcttggt gattaactgc aagctcataa
     1381 ctagttagat ttgcatctaa agcagcttct tctaatattt taaaagtaac tactgtattt
     1441 tcagaaaatc ttaaaacaaa atgttcttca actgtctcaa atcttctcgc aaatgtaata
     1501 ccatcaattt cataaaaata accagttata tttttattat tagaaactat tatctctaca
     1561 taagtagtct cactactctt agttatttga agttgattac caacaagttt cacatcagct
     1621 aatttagtaa aatctttatt ttctaaagat ttcttgtata caccatctct atatctaaat
     1681 ccatctagtt ccttatttaa actttgataa gcatcaagat ctctaatttc ggccctcaat
     1741 ccttcttcag tttttaaata aactactgca tcatcatatg gatacattgc atatttttga
     1801 ctagaactta cattatcaga taaatgtact ggaattgcta gagtaattat tagcactatt
     1861 acaactttca taatagtgca gtaaactcct ctagttctat ctaatactaa actcttcttt
     1921 cttcttctgt tcataaaata gaaagtatct aaatatttaa atttatctaa gtttgaaact
     1981 gcaaatacta atgcataaat cgaaataata accgcaacta aataagcact cttaattcca
     2041 agcgcagcaa cagctaaaaa gataaccgga acagctaatg gaatagttag tgtaagaatt
     2101 gttagactaa aacgaatatt aaacgaatct aatttaggga acaattcttc taaaagctct
     2161 ctaataatca taaacattgc aagatatgca aacaaataac taaccatagg taaaaagata
     2221 tttattaaaa ctgcaataaa tgtaattaca taagttaatc cttttcttaa agattttctt
     2281 tctaacttaa accaagtagc tcccttagct atagctaaag atgcaaatag tgccgtcatt
     2341 agaataagaa gaattgtaat taagttattg ttataagtga tatctgaaat cctatggaag
     2401 ttgacagttc caaataacgc agcaataaaa gcactcacat aatacaatac aaaaattgcc
     2461 aacactacaa agaacaaact accaacaata acagcaatac tcttaattgt atctttataa
     2521 tttgtttgtt tgcgataaat agtaataata attaaagcta taaatagcaa taaaagcaaa
     2581 ctatttaaag cataagcaaa acctttagaa taagaaactg ttcctaaatt taaataacta
     2641 aaataaacaa catcatcatc tgttaagtat agttcattta aattatagtc ttttgtagct
     2701 tctactgttg atgtaacaat attagcagct tggctaatat atgatttagg taaattctct
     2761 ctattatcat tttgagtatg ataatgctct ccaccgccaa tatttgctat attgtaacct
     2821 ttaatttcac ttgcaaacga agtgaaatct gtaaagttat tcatcatttg gtaaaccata
     2881 tttgaaattg atgacacaaa tggcgtttga tctaaatctt tatatagttt aattaattta
     2941 ctattcttct gactagtctc aaacataata gcagtcccac tactaccacg agattctaag
     3001 ttaatcccca gtttagttct ttggactaaa ttattgaaac cagtaaaatc actaacgtat
     3061 tttgcagatc ccaaacaacc atattcttcg ccatcagtaa aaacaaatac taaatcatta
     3121 ttagtaacta tttcattttt agttaatttg attttataat acctaattgc ctctaacatt
     3181 acagcaacca ttgaaccatc atcagcactt cctggcccca ttggcacaga atcataatgc
     3241 gccataaaca ttacggcatc attagttttt tcagcaccat tagcaggcaa ataaacaaca
     3301 atattttcta aaacaaagtc atgataagca acactatctt gagggtctaa accttcaact
     3361 attgtttcta aagtttctct gtttttatct actttctcgc ttgatgtttt ttggattaaa
     3421 tacgaataac ccaaaacatt ttgattaaca aaaccataac cttcaattgt atttacgata
     3481 tagtcacgag cagcttcttt tgcctcttta tcaataattg gtctagattc taagttatcc
     3541 aagtgagtat agatattatc tttatttaat tcagttttgt attcaacttc attaacatca
     3601 attatcgaaa aaacaagaac agcaataaat ataattgaga taattatatt atatatttta
     3661 gcaaatttct tcataacttt ctccctatct ctttaaagtt aattatgctt ctcttttctt
     3721 acctaagaaa aataaagcga tattaccagg tccaccgtga atgccaataa taggaccaaa
     3781 agatgttaga atagtatttt taactcccaa ttctttttca agagcatgct tcgtttcctt
     3841 tgctcctttt tcatcatcgc catggccgat accaatgatt tgattaatcg gatctattat
     3901 cttttctttt acaatctcaa gaattcttgc taaagccttt tttctgccac ggtgacgttc
     3961 aacaaccgta acttcccctt ttagattagc tgtacaaaca ggttttactt ttaataaaga
     4021 tgcaataaat gctttagtgc cagaaagtct tccccctctt ctgatatacc ataaatcatc
     4081 aattacaaag aaatgatgca tatgaacagc catttcattc atcttctctt ctactaaatc
     4141 ccagtccata ccctcatcta acatttcctt agcatagata gcaagcaaac cttcagctaa
     4201 tgatccagaa agtgagtcta caatcttaat tgttctttct gggtacatat cttctaactc
     4261 taacttagcc attctagcag cattaaacgt tcctgataat ccactagaaa ttgccatata
     4321 gaaaatatca tatccttgct ctaaataagg actaaagcct tcaataaatt cacttacatt
     4381 tacaagtgat gttttcaaat tctttttatc tctaattgca tcccacattt ttcttttttt
     4441 agcttcatca aagtattctg taattacttc ttcttcaata gttaccgtta aggcaacagt
     4501 cttaaaatca tatttttcaa tatattttac cggtagatca caacctgaat ctacaaacat
     4561 tacaaatttt ctcatatttc ttatctcctt ttaattttaa gtaatggagc agataacgag
     4621 gatcgaactc gcatattcag cttgggaagc tgacattcta ccattgaact atatctgcaa
     4681 cattaggtat tatatcattt tataaagtct ttgtaaagaa atgtaaatct tctttcctta
     4741 tc
//
LOCUS       DAOSGS010000066         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_16837_length_4591_cov_3.561949, whole genome shotgun sequence.
ACCESSION   DAOSGS010000066 DAOSGS010000000
VERSION     DAOSGS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16837_length_4591_cov_3.561949"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLQ22_03300"
     CDS             <1..703
                     /locus_tag="PLQ22_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59019.1"
                     /translation="FNNCYCEFIFDYNPTVDDFNGGGIMKAKLEKIYPVLVGLVSLVS
                     LLPLIVYAIDKFSQIFVILITVSILLLGFYLLNQTFGKSLDKRAKSILDLVLLITGFI
                     INIFLLASLKRYFYYEILLYFCLVTLVIKIATVVLEIFGLRKVNYFILLITPLVLGLV
                     YAILYISKYEFKYFTSYLYLLTPLIFWFSTSLYTLLFFKGKEDKGMVLLGMASYALLL
                     VILFEVLLFECYILG"
     gene            822..1376
                     /locus_tag="PLQ22_03305"
     CDS             822..1376
                     /locus_tag="PLQ22_03305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH59020.1"
                     /translation="MKELLLASRSRRRKEVMDFSGLKSRIVVALVDDKVEDVSPLEEA
                     QILAKRKCDYVHRQYPNDIVIGVVSVVNLEGKSLPKPKDEEHAKEILRLLSGKTHEVI
                     TACYLKYKDKVEYVDAVTEVTFADMSEDEIEYYVNTGEVFDKPGAYSAKGFGSRYIKK
                     IDGDIYNTYGLPVYQVYSALKKLW"
     gene            1431..2927
                     /locus_tag="PLQ22_03310"
     CDS             1431..2927
                     /locus_tag="PLQ22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOH59021.1"
                     /translation="MRKLILEKFKDSLSAVLPVSLIILVLIIGFFKVDLGNILMFVFA
                     AICLIIGIAIYNLGIDLSLSRVGEKIGAAIIKKRKIWLFVFFGLVLGFLITVAEPNLS
                     VLSVQTGIDRLVLIVSIGIGIGVFLVIALLRIVFQIPIRYILLGGYALVFILGIFLPE
                     KFVPMAFESGGVTTGPLTVPFIMAIGVGVAYIRGDKGSKDDNFGLIGTSLIGAIIAVM
                     ILGLIYDSQGKLDSLSASPSTLHEINSVSDFFHVVSSEAVIHLKEVAVALLPMLVLYL
                     VMHFIFFKDASRVVWTIIGGFAITYLGLTVFLIGANTGFLAIGGYLGQKIASYNKWIL
                     VPLALVLGTVIVLAEPSVGTLKSQVRDVTDGAISSKTLMAFISVGVGIAVALAMLRAI
                     TGISIWWIIVPTYALSLGLSFIVPKLFTAIAFDSGAIVSGPLAAAFLLPMAIGATTAV
                     GGDVFTDAIGIVAFVAMAPPLTIQILGLIYKFKEKAERVDYQEEVIYF"
     gene            2939..3271
                     /locus_tag="PLQ22_03315"
     CDS             2939..3271
                     /locus_tag="PLQ22_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59022.1"
                     /translation="MEKHKMLVLIINSKMEKKASKLIYKISPSMQFIHIGKGTAELAV
                     LDELGVGREPKVIMIAIIPSSLITEVLDKLDEGLNLHLPNSGIAFTLDLTAVSGLAVL
                     KYLTGRKE"
     assembly_gap    3410..3509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3510..3777
                     /locus_tag="PLQ22_03320"
     CDS             <3510..3777
                     /locus_tag="PLQ22_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOH59023.1"
                     /translation="EENVALEKHIPAVEVKGCKVTVTVGDVIHPMTEGHYIQFVYLLT
                     DKRLHKYYFNPGEHPSYTFKLEKGEKPLAAYEYCNLHGFWKKEI"
     gene            3777..4088
                     /gene="trxA"
                     /locus_tag="PLQ22_03325"
     CDS             3777..4088
                     /gene="trxA"
                     /locus_tag="PLQ22_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH59024.1"
                     /translation="MMAKEIGAKEFKELVLNADKPVIVDFFATWCGPCRMLAPVLDDL
                     AKDANGSFEVYKVDIDKDRALAMEYGVMSIPTIIAFQGGKQINKHIGYATKDQLKKMV
                     Q"
     gene            4231..>4591
                     /locus_tag="PLQ22_03330"
     CDS             4231..>4591
                     /locus_tag="PLQ22_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59025.1"
                     /translation="MKLFKRIVLVLALVLGVAVLTACSCKEEKKFSEEKITVYTRDTT
                     SGTRDGFFTGIGFKEAATDNAPLVAGFVEVTGNGDMIAKIQNDEYGIGYISLASYADS
                     GLKGLKYEGVEPTEANVL"
BASE COUNT         1413 a          518 c          923 g         1637 t
ORIGIN      
        1 ttttaataat tgctactgtg aatttatttt tgattacaat cctaccgttg atgattttaa
       61 tgggggtggc atcatgaaag ctaagttaga gaaaatttat ccagttttag ttggtttagt
      121 tagtttagtt tcactattac cactaattgt atatgcgatt gataaattta gtcaaatatt
      181 tgtgattttg attacagtat caatattgct tttaggattt tatcttttaa atcaaacttt
      241 tggtaaatct ttagataaaa gagcaaaaag cattttagat cttgttcttt taataacagg
      301 tttcattatt aacatttttc ttttagcttc tttgaaacgt tatttttatt atgagatttt
      361 attgtacttt tgtttagtta cattggtgat taaaatagct actgtcgttt tagagatttt
      421 tggtttaaga aaagttaatt attttatttt gcttattact cctttagttt taggattagt
      481 ttatgccatt ttatatatca gtaaatatga gtttaagtat tttacatctt atttgtattt
      541 acttactcca cttatttttt ggtttagtac atctttatat actttactgt tttttaaagg
      601 aaaagaagat aaggggatgg tgcttttagg aatggcaagt tatgctcttt tacttgtaat
      661 tttatttgaa gtgttattat ttgagtgtta tattttaggt taaattaaag aaaagaaaag
      721 aataagagct aatttattag ctcttatttt attttggaaa ataaaaaagc acttgaataa
      781 aacttgatat tttagtataa tagtgatagt tggaggtaga tatgaaagaa ttactattag
      841 catcaaggtc aagaagaaga aaagaagtta tggatttttc gggacttaaa agtagaattg
      901 tcgttgcttt agttgatgat aaagtggaag atgtttcgcc tttagaagaa gcacaaattt
      961 tagcaaaaag aaaatgtgac tatgtacatc gccaatatcc caatgatatc gtgattggtg
     1021 ttgtttctgt ggtgaattta gaaggtaagt ctttaccaaa accaaaagat gaagagcatg
     1081 ctaaagagat tttaagactt ttaagtggga aaacgcatga agttattaca gcttgttatt
     1141 tgaaatacaa agacaaagtt gaatatgtag atgcagtaac ggaagttact ttcgctgata
     1201 tgagtgaaga tgagattgag tattatgtca atacgggcga agtttttgat aaaccgggtg
     1261 cttatagtgc taagggattt ggaagccgtt atattaagaa aatcgatgga gatatttata
     1321 acacttatgg tcttccagta taccaagtat attcagcttt aaaaaagcta tggtagttta
     1381 ataagccttc ttggaagaag gcttctttat ataagaaagg agtttaagac ttgagaaaat
     1441 taatattaga gaaatttaaa gattcgcttt ctgctgtatt acctgtatct ttgattattt
     1501 tggttttaat cattggtttt tttaaggttg atctaggtaa tattttgatg tttgtttttg
     1561 cagctatctg tttaattatt gggatcgcta tttataactt aggtattgat ttatctttat
     1621 cacgggtagg ggaaaagatt ggagcagcta ttatcaaaaa acgaaagatt tggctttttg
     1681 ttttttttgg tctagtttta ggttttttga ttactgttgc tgagccgaat ttgtctgttt
     1741 tatctgttca aacagggatt gatcgtttag ttttgattgt cagtattggg attggaattg
     1801 gtgtcttttt agtcatagct ttactgcgaa tagtttttca aattccgatt agatatatct
     1861 tgttaggtgg ttatgctctt gtttttattt tagggatttt tttaccagaa aaatttgttc
     1921 caatggcttt tgagtctggt ggggtaacaa cagggccttt aactgtacct ttcattatgg
     1981 ctattggtgt aggtgttgcc tatattagag gtgataaagg ttcaaaagat gataactttg
     2041 gtttaattgg gacgagttta ataggagcaa ttattgcggt gatgatttta gggcttatct
     2101 atgatagcca aggtaaactt gatagcctat cagcttcccc ttctacgctt catgaaatta
     2161 atagtgttag tgattttttt catgttgttt caagtgaagc tgtgattcat ttaaaagaag
     2221 tagcagttgc cttactgccg atgttagttt tatatttagt gatgcacttt attttcttta
     2281 aagatgcttc cagagttgta tggacaatta ttggtggttt tgcgatcact taccttggtt
     2341 taacggtttt tttaattggt gctaatacgg gctttttagc aatcggaggc tatttaggtc
     2401 agaagattgc tagttacaat aaatggattt tagtaccttt agctttagtt ttaggaacag
     2461 ttatcgtttt agctgaacca tcggtaggga ctttgaaaag tcaagttcgt gatgtaactg
     2521 atggtgctat ttcttctaaa actttgatgg cttttatttc tgtaggggtg gggattgctg
     2581 ttgccttagc aatgcttaga gcaattacag ggatctcaat ttggtggatt attgtcccta
     2641 catatgcttt aagtttaggt ttaagcttta ttgtccctaa gctctttaca gctattgctt
     2701 ttgattcagg ggctattgtt tcaggtcctt tagcagcggc atttttattg ccgatggcaa
     2761 ttggagcaac gacagctgtt gggggagatg tctttactga tgctatcggt attgtagctt
     2821 ttgtagcgat ggcaccacca ctaacgatcc aaatcttagg tttaatctat aagtttaaag
     2881 aaaaagctga aagagttgac tatcaagagg aagttattta cttttaggag gcttagttat
     2941 ggaaaagcat aaaatgttag tattgatcat caatagtaaa atggaaaaga aagcaagtaa
     3001 actaatttat aaaattagtc cctcaatgca gtttatccat attggtaaag gaacagctga
     3061 gcttgctgtt ttagatgaat taggtgtagg aagagagccg aaagtgatta tgattgcgat
     3121 tattccttca tctttaatta cagaagtatt agataagtta gatgaagggt taaatctaca
     3181 tctacctaat agcggaattg cttttacttt agacttaact gcggttagcg gtttagctgt
     3241 tttaaagtat ttaacaggaa ggaaggaata aaatgaaaaa aatagctgcg caattagtga
     3301 ttgtaatttg taattatgat tatgttgatg atgtgatgga agttgcaaag aaagttgggg
     3361 cacgtggggg aacgattgtt catggtcgcg ggacaatgac gaaagaaacn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gaggaaaatg ttgctttaga aaaacatatt
     3541 cctgcagttg aagtgaaagg atgcaaagtt actgtaacag ttggtgatgt tattcatcca
     3601 atgactgaag gacattatat ccaatttgtt tatctactaa cagataaaag attacacaag
     3661 tattatttta atccaggaga acatccaagt tatacgttta agcttgaaaa aggagaaaaa
     3721 cctttagccg cttacgaata ttgtaattta cacggattct ggaagaagga gatttaatga
     3781 tggcaaaaga aattggtgca aaagaattta aagaattagt attaaacgca gataaaccag
     3841 ttatcgtaga cttctttgca acatggtgtg gtccttgtag gatgcttgct ccagtgttag
     3901 atgacttagc taaagatgct aatggtagtt ttgaagttta taaagttgat attgataaag
     3961 atagagcatt agcaatggaa tatggtgtta tgtctatccc aacaatcatc gcttttcaag
     4021 gtggaaaaca aataaacaag catattggtt atgcaactaa agaccaactt aagaaaatgg
     4081 ttcaatagtt taaataaatg gctacttttg taaataaaag tagctttttt attttacttt
     4141 ctctttacaa atttattact aaaactacgt tttgataaga cttgataaac gttataattg
     4201 acctgtaata agtaaaaaaa ggagaaagat atgaaattat ttaaaagaat tgttttagta
     4261 ctagcattag tattaggcgt agcagtatta actgcttgta gttgtaaaga agaaaagaaa
     4321 ttttctgaag aaaaaatcac agtatacaca agagatacta cttcaggaac aagagatgga
     4381 ttctttacag gaatcggttt taaagaagct gcaactgaca atgcaccact agtagcgggc
     4441 tttgtagagg ttacaggtaa tggtgatatg atcgctaaga ttcaaaatga tgaatatggt
     4501 attggttata tttcactagc atcttatgca gattctggat taaagggttt aaaatatgaa
     4561 ggggtagagc caacagaggc taatgtatta a
//
LOCUS       DAOSGS010000067         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_16958_length_4571_cov_3.731621, whole genome shotgun sequence.
ACCESSION   DAOSGS010000067 DAOSGS010000000
VERSION     DAOSGS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16958_length_4571_cov_3.731621"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="plsX"
                     /locus_tag="PLQ22_03335"
     CDS             <1..859
                     /gene="plsX"
                     /locus_tag="PLQ22_03335"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOH59026.1"
                     /translation="PNKRVTIVHTPRVISMGEADPRREIRNNKDTSLVQAFTAVKENQ
                     AEGVVTAGPTQAVVVSAHIVLRRMKGMSRMALCLGLPDLGDGKQRLLLDSGANLELKP
                     EHLVQLAEYATIYMKEVMGVEKPVVGLLNIGTEEAKGREFDIEAFHLLKDNKNVTFFG
                     NIEPKEMLASPCDILVTDGYTGNMVMKTAEGVAIAVGKLLKQGIMASSKAKFGYLFMK
                     GVFKELKNKTTADSIGGAILIGADGIVIKAHGSSNGPSFQAAISLARRAVLGNVNEKM
                     KAIIGDKDE"
     gene            852..1535
                     /gene="rnc"
                     /locus_tag="PLQ22_03340"
     CDS             852..1535
                     /gene="rnc"
                     /locus_tag="PLQ22_03340"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOH59027.1"
                     /translation="MNKVFKAICLKYDLKVNNYELYQTAFTHTSYANENNVKNNERLE
                     FLGDAILNFCFSEYLYRKYPNLREGEMTKIRATYVCEQANAEYSLKLGLDKLLLLGKG
                     EEEQNGRSKPSVLGDLFEAFLGAVYLDSSLEKVKEIINDLVLKEIDTIKEDGFFLDYK
                     STLQEYVQAETKKSVTYVLVKEEGPSHDKLFTTFVYYEKIKLGEGIGKSKKESEQNAA
                     KAALEKLAK"
     gene            1546..2160
                     /locus_tag="PLQ22_03345"
     CDS             1546..2160
                     /locus_tag="PLQ22_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH59028.1"
                     /translation="MEKYYDLIKNNLINFDNLIIEKYASLKLNETEAMILIKINRLLQ
                     RDIKSISVKKLVKNMTINENDLANYLMRLIDFGFLKIDFEAQRELYSLDGTYQKLANL
                     LYGEDNNQENKLKENRLVATTKKLEQGFNKVLSPIEVEIVRKWFYEFEYDYKDIEEFV
                     NSAISSKNRGVKYIDRALVNRYSNKADEDMDALKDLWKDYHGKN"
     gene            2147..2776
                     /gene="nth"
                     /locus_tag="PLQ22_03350"
     CDS             2147..2776
                     /gene="nth"
                     /locus_tag="PLQ22_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH59029.1"
                     /translation="MEKIDYICDALGELFPNNKGDLEYGSVFQLLVAVILSAQTTDIA
                     VNNVTKELFKKYPNAKSLAKADIEDVKEIIKTIGLYNTKAKNIIEASKIILENNNVIE
                     PDIKWLMGLPGVGRKTANVVLSEGFKIPRIAVDTHVFRVARRLELSKAETTLGVEEDL
                     MKILDQKKWGIAHLRLLRFGRYICKARGPLCEKCPFFHFCTSEDKKELV"
     gene            complement(2777..4351)
                     /locus_tag="PLQ22_03355"
     CDS             complement(2777..4351)
                     /locus_tag="PLQ22_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOH59030.1"
                     /translation="MSFDGVFIRNLIKELKTKIINIRINKVNFYNKYDFSFSLSNKQE
                     LLYLLSNNPHLLLTKQSLVKSDNQSNFSKVLKKLFEKAVIMDIYQIENDRIIVLEISS
                     FDNLGYEEKYKLIFEAFGRNYNLIIVNKENKILEILLPTSIDSKRILLKNTLYEPMDS
                     NQINPYKTNILTKTNNYQGISQILFQEIQYQNDLNVINNEVKPVIIDNDKTYFYCFDL
                     KHLKGNRMYFSTLSELLESYFQNFKNQSIINNDLKLLSNHLDKELAKLRRKKGKQEKE
                     LEEAYNNLEYEQIGNILKANLHQIKKGMSKLTTNNFYTNKEITIKLDPLLSPSQNLDQ
                     IFKKYKKAKRSIDYIKKQIEESQLDILYYEDIQSQLDYFDYNSINETILELGLRKITK
                     KDEKDLKKPKYTTYILDDNKFIYLGKNNFQNSFVTHKLANKGDYFFHVKDYPGSHVIL
                     RGSNLLEDDFKIAANFAAYFSKAQNSSNVAVIYTQVKNVSKLAGQKGSFVKYKNEKTI
                     FVTPNKDLIARYEKRN"
     gene            4487..>4571
                     /locus_tag="PLQ22_03360"
     CDS             4487..>4571
                     /locus_tag="PLQ22_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH59031.1"
                     /translation="MKLDDKGILIVLSGPSGVGKGTIRKALF"
BASE COUNT         1602 a          511 c          782 g         1676 t
ORIGIN      
        1 tcctaataag agagttacta ttgttcacac acctagagtt atttccatgg gtgaagctga
       61 tccaagacgt gaaattagaa ataataaaga cacttcttta gttcaagcat ttacagctgt
      121 aaaagaaaat caagctgaag gtgtagtaac tgcaggtcct acccaagctg ttgttgtatc
      181 agcacatatc gttttgagaa gaatgaaagg aatgagccgt atggctttat gtttaggact
      241 tcctgattta ggtgatggca aacaaagatt acttttagat tcaggtgcta acttagagtt
      301 aaaaccagaa catttagttc aacttgcaga atatgcaact atttatatga aagaagtaat
      361 gggtgttgaa aagccagtag taggtctttt aaatatcgga acagaagaag caaaaggtag
      421 agaatttgat attgaggctt ttcacttatt aaaagataat aaaaacgtta cattttttgg
      481 aaacattgaa ccaaaagaga tgcttgcatc tccttgtgat attttagtaa cagatggtta
      541 tactggtaat atggtaatga aaacagctga aggtgttgca attgcggttg gtaagctttt
      601 aaagcaagga attatggcat ctagtaaagc taaatttggt tacctattta tgaaaggtgt
      661 tttcaaggaa ttaaaaaata aaactactgc tgatagcatt ggtggagcaa ttttaattgg
      721 tgctgatggg attgttatta aagctcatgg ttcatcaaat ggtccatcat ttcaagcagc
      781 aattagtctt gcaagacgtg ctgttttagg taatgtcaac gaaaagatga aagcaatcat
      841 tggtgacaaa gatgaataag gtgtttaagg caatttgttt gaagtatgat ttaaaggtaa
      901 ataactatga attgtaccaa actgccttta cccatacatc atatgccaat gaaaataatg
      961 taaagaataa tgaaagatta gagtttttag gtgatgcaat tttaaatttt tgtttttctg
     1021 aatacttgta tcgtaaatat cctaatttac gagaaggtga aatgacaaaa attagagcaa
     1081 cttatgtatg tgagcaagca aacgcagaat acagtttgaa gttaggctta gacaaactct
     1141 tacttttagg taaaggcgag gaagaacaaa acggaagaag taaaccttct gttttaggag
     1201 atttatttga agctttttta ggagctgttt atttagattc ttctttggaa aaagtcaaag
     1261 aaattattaa tgatcttgtt ttaaaagaaa ttgatactat taaagaagat ggctttttct
     1321 tggattataa gagtacttta caagaatatg ttcaagcaga aacaaagaaa tctgttactt
     1381 acgtattagt aaaggaagaa ggacctagtc atgataaact ttttacaaca tttgtttatt
     1441 atgagaaaat taaattaggc gaaggtatag gtaagtcaaa aaaagaatct gagcaaaacg
     1501 ctgctaaagc agctttagag aaattagcaa agtgaggttt tagttatgga aaaatattat
     1561 gatttaatta aaaacaatct aataaatttt gataatttaa ttattgaaaa atatgcatcc
     1621 ttaaaactta atgaaactga ggcaatgatt ttgattaaga ttaatcgttt actccaaaga
     1681 gatattaaat ctatttctgt caagaaattg gttaaaaaca tgacgattaa tgaaaatgat
     1741 cttgcaaact atttaatgcg attaattgac tttgggtttt taaaaataga ttttgaagct
     1801 caaagagagt tatattcgtt agatggtacg tatcaaaagt tagctaactt actttatggc
     1861 gaagataaca accaagaaaa caaacttaaa gaaaatagac ttgtagctac tacaaaaaaa
     1921 ctagaacaag gatttaataa agtcttatca cctattgaag ttgagattgt tcgtaaatgg
     1981 ttttatgaat ttgaatatga ttataaagat attgaagaat ttgtaaatag tgcaatttct
     2041 agtaaaaata gaggtgttaa gtatattgat cgtgctttag taaatagata tagtaataaa
     2101 gcagatgaag atatggatgc attaaaagac ctttggaaag actatcatgg aaaaaattga
     2161 ttatatctgt gatgctttag gagaactttt tccaaacaat aaaggtgatt tagaatatgg
     2221 ttcagttttt cagttattag ttgccgttat tttatcagcg caaactacag atatagcagt
     2281 taataatgta actaaagaat tatttaaaaa atatcctaat gcaaaaagct tagctaaagc
     2341 tgatattgaa gatgtaaaag agattattaa aactattggt ttatataata caaaagcaaa
     2401 aaacattatt gaagcatcta aaatcatttt agagaacaat aatgtaattg agcctgatat
     2461 aaagtggtta atgggtcttc caggtgtagg gcgaaagaca gctaacgtag ttttatctga
     2521 gggatttaaa attccaagga tagcagtaga tactcatgtt tttagggtag cgagaagatt
     2581 agagttatca aaggcagaaa ctactttggg tgttgaggaa gacttaatga aaatattaga
     2641 tcaaaaaaaa tgggggatag cgcatttacg attactacgt tttggtagat atatttgtaa
     2701 agcaagaggt cctctatgtg aaaaatgccc tttttttcat ttttgcacct ctgaagataa
     2761 aaaggaatta gtttaactaa ttcctttttt catatctagc tattaaatct ttgtttggtg
     2821 taacgaagat tgttttttca tttttatact ttacaaatga tcctttttga cctgctaatt
     2881 tacttacatt ttttacttga gtataaataa cagctacatt gctactattt tgcgcttttg
     2941 agaagtatgc agcaaagtta gcagctattt taaaatcatc ttcaagtaaa ttgcttcctc
     3001 ttaaaattac atgagatcct ggatagtctt ttacatgaaa gaaataatca cctttattag
     3061 ctagtttgtg agtaacaaaa ctattttgaa aattattttt acctaaatag atgaacttat
     3121 tatcgtccaa aatgtaagta gtatatttag gtttttttaa atctttttca tctttttttg
     3181 tgatttttct taagcctaat tccaagattg tttcattaat gctattataa tcaaagtagt
     3241 ctaattgtga ttggatatct tcgtagtata aaatatcaag ttgagattct tcgatttgtt
     3301 ttttgatata atcaatactt cttttagctt ttttgtattt tttgaatatt tgatctaagt
     3361 tttggctagg tgataaaaga ggatcaagtt taattgtaat ttctttattg gtgtaaaagt
     3421 tattggtagt tagcttactc atcccttttt taatttggtg aaggtttgct tttagaatat
     3481 taccaatttg ttcatattct agattattat atgcttcctc taattctttt tcttgttttc
     3541 ctttttttct tcttaactta gctaattctt tatctaaatg attagaaagt agttttaagt
     3601 cattgtttat aattgattgg tttttaaaat tttgaaagta agattctagt aattctgata
     3661 gtgtagaaaa atacattcta ttacctttta ggtgttttaa atcaaaacag tagaagtatg
     3721 ttttatcatt atctataatt actggtttaa cttcattatt tataacattc aaatcatttt
     3781 gatattgaat ttcttgaaaa agtatttgcg atataccttg atagttattt gtttttgtaa
     3841 ggatatttgt tttatatgga tttatttgat ttgaatccat aggctcatat aaagtatttt
     3901 ttagtaagat tcttttacta tctatagaag taggaagtaa gatttctaaa attttgtttt
     3961 ctttgtttac aataattaaa ttatagtttc tgccaaaggc ttcaaaaatt agtttatatt
     4021 tttcttcata gcctagattg tcaaatgaag agatttctaa tacgatgata cgatcatttt
     4081 caatttggta gatatccata ataactgctt tttcaaatag tttttttaag actttagaaa
     4141 agtttgattg attatcactt tttactagtg attgtttggt aagtagtagg tgagggttat
     4201 ttgatagcaa gtaaagtaat tcttgtttat tcgacaagct aaaagaaaag tcatatttat
     4261 tataaaaatt gactttgttt attcttatat taatgatttt tgtttttaat tctttaatta
     4321 aatttctaat aaatacgcca tcaaaactca taacttcacc ttaatttatt ttaccaaaaa
     4381 ttcactctat ttgcaattat agttgttgat gatctttgaa aaaccattaa catttgacaa
     4441 tctattcttt tttaggtata ataagtggta atatgaggaa tgttttatga aattggacga
     4501 taaaggaatt ttgatagttt tatctggacc aagtggtgta ggtaaaggga caattagaaa
     4561 agctttattt g
//
LOCUS       DAOSGS010000068         4547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_17081_length_4547_cov_4.499555, whole genome shotgun sequence.
ACCESSION   DAOSGS010000068 DAOSGS010000000
VERSION     DAOSGS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17081_length_4547_cov_4.499555"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLQ22_03365"
     CDS             <1..1147
                     /locus_tag="PLQ22_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59032.1"
                     /translation="DLKDILEETYGVIVFQEQVMLILQKIGGYSLGEADVVRRAISKK
                     DKALMEKERTKFIARSKKMGYSQNIGEQIFDLIFEFASYGFNKSHATAYALVAYQMAY
                     LKTYYYKYFMTVLMSYNLGSSSLNQDYIYEAKSKGLVIKSPDINISKDKFVFKDQYIY
                     YPLLGVKGIGEITCKDLLEERKANGKYEDYYSFIERTKDILSETNVMTLIDSCSLDSF
                     GLTHKFMVEEYNNAYNIAKFSSAFKSKMVRKEPSKDEFSLEENNLREKEALGFNLKYS
                     LFGGYEHIKRKYNLPNLNQLTVRRNQQLLFAIKRIKIIKTKNNEEMAFLEIYDDTAII
                     DAVIFPATYQKYQGILEEKKIYLGLGNLEMRKGKMQYIVNELTRRIQ"
     gene            1144..2097
                     /gene="pfkA"
                     /locus_tag="PLQ22_03370"
     CDS             1144..2097
                     /gene="pfkA"
                     /locus_tag="PLQ22_03370"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH59033.1"
                     /translation="MKRIAILTSGGDAPGMNAAIRAVVRACKIKNIRVYGVRNGFQGL
                     LDEDIEELNRKSVSEKLSKGGTFLGTARVADFYKEEVQAKAADNLKRRGIDGLVTIGG
                     DGTFRGALGLSKKGISVIAIPGTIDNDIEGTDFTIGFDTALQTAVEAVDKLKDTSSSH
                     RRCSIVEVMGRECGDLAIWTGIASGAEFVVAKEVGYHIDDIIENVREAAEVKNHAIII
                     VAENMVDINELAKVISEKTPFSARTTILGHIQRGGTPTARDRVLASQMGAKAVDALLE
                     GESGNAVCIRNNQIITVPIEDALRKDDSQALELYELLRELW"
     gene            2091..3680
                     /gene="metG"
                     /locus_tag="PLQ22_03375"
     CDS             2091..3680
                     /gene="metG"
                     /locus_tag="PLQ22_03375"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH59034.1"
                     /translation="MVKMKKTAYITTPIYYASGKVHIGNAYTTIAADAFARYNRLVGR
                     DTFYLTGMDEHGLKIEEAAKKNKMEPQAFVDRIAEETKATWKDLKISYDYFIRTTDDC
                     HVKAVQGIFEKLLKQGDIYLGKYEGNYCMSCEAFFSDSQLVDKVNCPDCGRPTTLLDE
                     ESYFLKLTKYADKLLKHIEDHPDFIQPESRKNEVVSFIESGLEDLCVSRQSFKWGVPV
                     LSNPNHVVYVWIDALSNYITALGYGSSDTSNYEKYWINGTETIHIIGKDILRFHAVYW
                     PIMLFALGEPVNFKVYTHGFILMKDDKMSKSKDNFVYPHEIIDAYGLDSLRYYLLREM
                     PFGNDSSFTYERFIDRYNSDLANDLGNLVSRTVSMVNKYFGGSVSYKDAKDEHSKELK
                     DLAEKVSENYQNHFSKFHIQEAIIEAFSLVSRANKYVDEMAPWVLAKAEDQKETLEEA
                     MYHLLETIRVVNILLSPVLVDAAEDIKEILNLSKKDLEFKNITFGDLKEYKIKEKAKI
                     LYKRLDKEKELEFQEERLKNR"
     gene            3698..4285
                     /locus_tag="PLQ22_03380"
     CDS             3698..4285
                     /locus_tag="PLQ22_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59035.1"
                     /translation="MNQETRVGKVNRNIFFIIVLLVLSLLTSCGCTRRKEFFNQSELK
                     EYGLTGLEVPNNSSNYYNKSLSYRLICYMRVSTDDDVISYATDILNMLKNQEIYKVYG
                     HKKLNDQREYEKNVYLSNDIDDYRTMTKKYSPDSVTLNNFAIFYALQDQKKEKTMFII
                     YLTSYTEEQTYYAAGFNFRIMVVKKPFSKYTIISE"
BASE COUNT         1731 a          471 c          866 g         1479 t
ORIGIN      
        1 tgatttaaaa gatattttag aagagactta tggagttata gtattccaag aacaagttat
       61 gcttatctta caaaaaattg gtggttatag tttaggtgaa gccgatgttg ttagaagagc
      121 tatttctaaa aaagacaaag ctttaatgga aaaagaaaga actaaattta ttgcaagaag
      181 caaaaaaatg ggatattctc aaaatattgg tgagcaaatt tttgatttga tttttgaatt
      241 tgcatcttat ggttttaata aaagtcatgc aacagcttat gctttagttg cttatcaaat
      301 ggcttatctt aagacttact attataaata ctttatgact gttttaatga gttataattt
      361 gggcagttca agtttgaatc aagattatat ttatgaagct aaaagtaaag gcttagtaat
      421 taaatcacca gatattaata taagtaaaga taagtttgta tttaaagatc agtatatata
      481 ttatccttta ctaggagtta aaggaattgg cgaaattact tgtaaagatt tacttgaaga
      541 aagaaaagct aatggtaaat atgaagacta ttatagtttt attgaaagaa ctaaagatat
      601 tttgagtgaa actaatgtta tgactttaat tgatagttgt agcttagatt catttggttt
      661 aacacataaa tttatggttg aagaatataa caatgcttat aatatagcga agttttcttc
      721 ggcttttaaa tcaaagatgg taagaaaaga acctagtaaa gatgaattta gtttagaaga
      781 aaacaatttg agggaaaagg aagctttagg ttttaattta aaatattcac tatttggtgg
      841 gtatgaacat attaagagaa aatataatct tcctaattta aatcaattga cagttaggag
      901 aaatcaacaa ttgctttttg cgattaaaag gattaaaata ataaagacaa aaaataatga
      961 agagatggca tttttagaaa tctatgatga tacagcaata attgacgctg ttatctttcc
     1021 agcaacctat caaaaatatc aaggaatttt agaagagaaa aagatttatt tagggttagg
     1081 aaatctagaa atgagaaaag ggaaaatgca atatatcgtt aatgaattaa caaggaggat
     1141 acaatgaaaa gaattgccat tttaacatct ggtggagatg caccgggaat gaatgctgct
     1201 attagagccg tagttagagc atgtaaaatt aaaaatatta gagtatatgg agttcgtaat
     1261 ggattccaag gtttacttga tgaagatatt gaagaattaa accgtaaatc agtttccgaa
     1321 aaactaagca aaggtggtac ttttttagga actgcaagag tagctgactt ttataaagaa
     1381 gaagttcaag caaaagcagc ggataattta aaaagaaggg gaattgatgg tttagttaca
     1441 attggtggcg atggtacttt tagaggggca ttaggtttat ctaaaaaagg aattagcgtt
     1501 attgcaattc ctggtacgat tgataatgat attgaaggta ctgacttcac aattggtttt
     1561 gacacagctt tacaaaccgc agttgaagca gttgataagc ttaaagatac atcaagttca
     1621 catagacgtt gttcaatagt tgaggttatg ggaagagaat gcggggatct tgcaatttgg
     1681 acaggaatag catctggtgc tgagttcgta gttgctaagg aagtaggcta tcatattgat
     1741 gatattattg aaaatgtaag agaagccgct gaagttaaaa atcatgcaat tattattgta
     1801 gctgaaaata tggttgatat taatgaacta gcaaaagtta tttctgagaa aactccattt
     1861 tctgctcgta caactatttt agggcatatt caaagaggtg gaacaccaac tgcaagagat
     1921 cgtgttttag catcacaaat gggagctaaa gcagttgatg cgttacttga aggtgaatcg
     1981 ggtaatgcag tatgtattag aaataatcaa ataattacag ttccaattga agatgcctta
     2041 agaaaggatg actctcaagc attagaatta tatgaattat tgagagagtt atggtaaaaa
     2101 tgaaaaagac agcttatatt acaacaccta tttattatgc aagtgggaaa gttcatattg
     2161 gaaacgccta tactacaatt gcagctgatg cttttgcaag atacaataga ctagtaggta
     2221 gggatacttt ttacttaact ggtatggatg agcatggctt aaagattgaa gaagcagcaa
     2281 agaaaaacaa gatggagccc caagctttcg tagacagaat agcggaagag acaaaagcaa
     2341 cttggaaaga tttaaaaata agttacgatt actttattag aactacagat gattgccacg
     2401 taaaggcagt tcaagggatt tttgaaaagt tattaaaaca aggagatatt tatttaggta
     2461 aatatgaagg taattattgt atgtcttgtg aggctttctt ttctgatagt caattagtag
     2521 acaaagttaa ttgcccagac tgtggaagac caacaacact tttagatgaa gaatcatact
     2581 ttttaaaatt aactaaatat gctgataagt tattaaaaca tatcgaagat catcctgact
     2641 ttatccaacc tgaatcaaga aaaaatgaag tagtttcatt tattgaaagt ggcttagaag
     2701 atttatgcgt aagtagacaa agttttaaat ggggagttcc agttttgagt aatcctaatc
     2761 atgtagttta tgtttggatt gatgctttat caaactatat tactgcctta ggttatggaa
     2821 gtagtgatac tagtaattat gaaaaatatt ggattaacgg aactgaaact attcatatta
     2881 ttggaaaaga cattttaaga ttccatgctg tttattggcc aataatgtta tttgctttgg
     2941 gtgaaccagt taattttaaa gtttatactc atggctttat tttaatgaaa gatgataaaa
     3001 tgagtaaatc aaaggataat tttgtttatc ctcatgagat tatagatgct tatggtttag
     3061 attcgctaag atattattta ttaagagaaa tgccttttgg taacgatagt agttttactt
     3121 atgaaaggtt tattgatcgt tataatagtg atttagctaa tgatttaggc aatttagtta
     3181 gtagaactgt atcaatggtt aataagtatt ttggaggtag tgttagttat aaagacgcta
     3241 aagatgaaca ttctaaagaa ttaaaagatt tagcagaaaa agttagcgaa aattatcaaa
     3301 accatttttc taagtttcat attcaagagg caattataga agcattttct ttagtaagta
     3361 gggctaataa atatgtagat gaaatggcac cttgggtact agctaaagca gaagatcaaa
     3421 aagaaacttt agaagaggct atgtatcatt tacttgaaac aataagagta gttaatatct
     3481 tattaagtcc tgtattagtt gatgcagctg aagatattaa agaaattcta aatttaagta
     3541 aaaaagattt agagtttaag aatataactt ttggggattt aaaagaatat aaaattaagg
     3601 aaaaagctaa gattttatat aaacgtttag ataaagaaaa ggaactggag tttcaagaag
     3661 aaagattaaa gaatcgctaa ataaaaaggg ggttattatg aatcaagaga ctagggttgg
     3721 caaagttaat agaaatatat tctttattat tgttttacta gttcttagtt tattaacttc
     3781 atgtggatgc acaaggagaa aagaattttt taatcaaagt gagttaaaag aatatggatt
     3841 aactgggtta gaagttccca acaattcgtc taattattat aataaaagcc ttagctatag
     3901 gttaatttgt tatatgagag tttctactga tgatgatgta attagttatg caacagacat
     3961 tcttaatatg ctaaaaaatc aagaaattta taaagtatat gggcataaga aattgaatga
     4021 tcaaagagaa tatgagaaaa atgtatattt atctaatgac attgacgact atagaacgat
     4081 gactaaaaag tattcaccag atagtgtgac acttaataat tttgcaattt tttatgcatt
     4141 acaagatcag aaaaaagaaa agacaatgtt tatcatatat ctaacatctt atacagaaga
     4201 acaaacatat tatgctgctg ggtttaattt taggattatg gttgtgaaaa aacctttttc
     4261 taaatataca attatatctg aataaaaaag ggttagcttt aattgctaac tcttttaaat
     4321 ataatgtttt taaaaaagaa aatatcttga cttttaagat gaaatatagt aatatgtata
     4381 aggtacattt taaaccccaa gattggccct gttataaatt taaattacag gagggaaata
     4441 tgagtaaatc atttatggct aataaacaaa acattgagaa aaaatggtat gttgttgacg
     4501 cgacagattt agttttaggt agattatcta ctgttgttgc tagtctt
//
LOCUS       DAOSGS010000069         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_17624_length_4457_cov_4.249886, whole genome shotgun sequence.
ACCESSION   DAOSGS010000069 DAOSGS010000000
VERSION     DAOSGS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17624_length_4457_cov_4.249886"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="PLQ22_03385"
     CDS             complement(<1..1701)
                     /locus_tag="PLQ22_03385"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOH59036.1"
                     /translation="MDSTTLYIVIATIGVGILAFIVAYFLYRWVKKLPSDNETIEKVG
                     GHIRRGANTFLKREYMVLAIFAGIVALLILLFLPEPIWVGGALENVLMAGFYLLGTLL
                     SAIAGKVGIMVATTANVKTAEAAKRGGITQSFLAGFRGGSVMGLAVVATCVIGISAIY
                     GLTTGVDSLNDFTASTMLGFSFGASSLALFAKAGGGIFTKTADISADLVGKVQYGIPE
                     DDPRNPAVIADNVGDNVGDVAGMGADLFDSNVAAIAAALVIAINLDDPGVRNNFIQTI
                     LVFSGLGLLSTIIGILFARMGKHDNPSRALNIGTYVTTGLFAASSALCTWLLGFSWYV
                     WGAAIVGLAVGTIIGIATDYYTNDVHKPVQQTAKLSEDGPAFTILSGVSYGFLSVLPA
                     MVGIAIAAVASFMLVQKSAPTHELAGTYGMFGISMAAVGMLSIVGIIVSNDAYGPIVD
                     NARGLAEMGGFGEEIVEITDKLDSAGNTVKAVTKGFSIGAAGLTVVSLLGAFISEVNE
                     MIGLGYTNAYGNTILPFEYFNITDPVVFFGIIIGAAVPAVFSALLMMGVNKNASRMVQ
                     E"
     gene            complement(1802..3130)
                     /locus_tag="PLQ22_03390"
     CDS             complement(1802..3130)
                     /locus_tag="PLQ22_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH59037.1"
                     /translation="MDIWLQLILLVILIFINGFFAASEMAIVSSNRLVIEEEADKGRK
                     KAKTLLKLLDDESKFLSTIQVVITLAGMFSASSAAVQLAVPVGRLFEKLGTSVNAGYT
                     IAIVVLTILVAFFNLVFGELVPKRVALIKKEKLAIAVAPVIMFFKVILTPFVWLLSGT
                     TNLVLRVFGYKRHEEEGEFTKEEMKRLLKAGKASGSLSDNESKMIASVFMLEQFDASD
                     IMTPRKLVYAIDVNDDIKKIIDEYIENGYSRVPVYEKDIDNIIGILYNKDLLRALKNK
                     GNHNINLKEILRPVYFVSETMKADKMFQEMQHNHSYIAIVIDEFGGFSGIVTLEDLVE
                     EIVGNIYDEDDQSDIRLVAEDTYLTKGIVPIQDINRTLDVEIPISTDDSYDSVGGLLI
                     YLLERLPNEEDKNKTLEFANLEFKIIDVKKNTINKVQIKILKQENIEEED"
     gene            complement(3192..4169)
                     /locus_tag="PLQ22_03395"
     CDS             complement(3192..4169)
                     /locus_tag="PLQ22_03395"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOH59038.1"
                     /translation="MKVIINQNTDPHFNLSVEEYIIKNYDLEEDIIMLWRNDKTVVIG
                     RNQNPYNEVNLEYLKANGITLARRLSGGGAVYHDLGNLNYTIFTKEKNSFNNYKKFCM
                     VIIETLKYFGIEANFEGRNDIYIDGAKFSGNAQYFYQDKMFHHGTILFSVDLNTLSKV
                     LVSKEKYEDKAVKSNKSKVVNLKEINSLITIEDVIDTLITKVLKLRKEDLYVLNEDEL
                     KEIEKIKNERFGLWEWNYGKNFSFQKKKKAKFKGGTIELTFEVRNGLINNVKVYGDFL
                     GVKTNSDLEELLINKPYNKEYIGKLFREEKVDNYFYLITSNEIIDLLFE"
     gene            4269..>4457
                     /locus_tag="PLQ22_03400"
     CDS             4269..>4457
                     /locus_tag="PLQ22_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOH59039.1"
                     /translation="MKVLLLVFSGTNNSFYVCNELKKEYQKLNIETDILRLENVKELK
                     DDYDLIGISYPIHAFSAPR"
BASE COUNT         1518 a          880 c          510 g         1549 t
ORIGIN      
        1 ctcttgcacc atacggctag cattcttatt aactcccatc attaatagag cactaaagac
       61 agcaggaact gctgctccaa taataatacc aaagaatact acaggatcag taatattgaa
      121 atattcaaat ggcaagatcg tattaccata agcattagta taaccaagac caatcatctc
      181 atttacttca ctaataaatg ctccaagtaa agatacaaca gttaatcctg cagcaccaat
      241 tgagaatcct tttgtaactg ctttaactgt atttcctgct gagtcaagtt tatcagtaat
      301 ctcgacaatc tcttcaccaa atccacccat ttcagctaag cccctagcgt tatctacaat
      361 tggaccataa gcatcattac ttacaataat accaacaatt gataacattc ctactgctgc
      421 catggaaata ccaaacattc cataagttcc tgctagttca tgagtaggtg cagatttttg
      481 tacaagcata aagcttgcaa cagcggcaat cgcaatacca accattgcag gtagaacaga
      541 taagaatcca taacttacac ctgaaagaat tgtaaatgct ggtccatctt cagaaagttt
      601 agctgtttgt tggacaggtt tgtgaacatc gtttgtataa tagtcagttg caataccaat
      661 aattgtacct actgctaaac caacaatagc tgctccccat acataccaag aaaaacctaa
      721 aagccatgta cataaagcac tagatgctgc aaataatcca gttgttacat aagtaccaat
      781 atttaatgct ctacttgggt tatcatgttt acccattctc gcaaataaaa taccgatgat
      841 tgttgataat aatcctagac ctgagaatac taaaattgtt tgaataaagt tatttcttac
      901 accaggatca tctaagttaa ttgctataac taaggcagct gcaatcgctg ctacgtttga
      961 gtcaaataag tcagcgccca ttccggcaac gtccccaacg ttatctccta cgttatctgc
     1021 gataacagca gggttacgtg gatcatcttc tggaattccg tattgaacct tacctactaa
     1081 gtcagcagaa atatctgctg ttttcgtaaa gattcctcca ccagcctttg caaacagagc
     1141 taaagaactt gctccgaaag aaaaacctaa catcgtactt gcagtaaaat catttaatga
     1201 gtcaactcct gtagttaaac catagattgc tgaaatacca attacacacg tagcaacaac
     1261 ggcaagaccc attacagatc cacctctaaa tccagctaag aagctttgag taattcctcc
     1321 tcttttagct gcttctgctg tttttacgtt agctgttgta gctaccataa ttccaacctt
     1381 accggcaatt gctgataata atgtacctaa taaataaaat ccggccatta gtacattctc
     1441 taatgcacca cctacccaaa taggttctgg taggaacaat agaataagta aagctactat
     1501 tcctgcaaat atcgcaagaa ccatatactc tctttttaag aaagtattag ctcctctcct
     1561 aatatgacct cctacttttt caattgtttc attatcagaa ggtaatttct taacccaacg
     1621 ataaagaaaa taagcaacaa taaaggctaa aatacctact cctatagttg caataacaat
     1681 ataaagtgtt gtgctatcca taaatcttca cctcgtttct taaatgaata acattcctca
     1741 ctattataac aaattgtgaa aacgatatca agcaaaaaat aaaaaaagac taacttttta
     1801 gttagtcttc ttcttctatg ttttcttgtt ttaaaatttt tatttgcact ttattaatcg
     1861 tatttttctt tacatctata atcttaaact ctaagtttgc aaactctaat gtcttatttt
     1921 tatcttcttc attaggtaat ctttcaagta aatagattaa taatccacct acagaatcat
     1981 acgagtcatc agttgaaatt ggaatctcaa catccaatgt acgattaata tcttggattg
     2041 gaacaatacc ttttgtaagg tatgtgtctt ctgctactaa tctaatatca ctttgatcat
     2101 cttcatcata gatattccca acaatctctt caactaagtc ttctaaagtt acaattcctg
     2161 aaaatccacc aaattcatca attacaattg caatataact atgattatgt tgcatctctt
     2221 gaaacatctt atcagctttc attgtctcag aaacaaagta aactggtctt aaaatctctt
     2281 ttaaattaat attatgatta cctttgttct ttaatgctct taataagtct ttgttataca
     2341 aaataccaat aatattatca atgtcctttt cataaacagg aactcttgaa taaccatttt
     2401 caatatattc atcaattatt tttttaatat catcattaac atcaattgca taaactaact
     2461 ttcttggagt cattatatct gatgcatcaa attgttcaag catgaatact gaagctatca
     2521 ttttactttc attatcactt aatgatccag aagccttacc tgctttaagc aatctcttca
     2581 tttcttcttt tgtaaattcg ccttcttctt catgtctctt ataaccaaaa actcttaaaa
     2641 ctaaattagt cgtaccagat aaaagccata caaaaggagt taagattacc ttaaaaaaca
     2701 taattacagg agcaactgct attgcaagtt tctctttttt aattaaagct actcttttag
     2761 gaacaagttc cccaaatact aagttaaaga aggcaactaa aatagtcaac accacaattg
     2821 caatcgtata acctgcatta acgcttgttc ccaatttttc aaataatcta cctactggta
     2881 cagcaagctg aactgctgca gatgatgctg aaaacattcc tgctaaagta ataactactt
     2941 gaattgtcga taaaaacttt gattcatcat ctaagagctt taataaagtt ttagcttttt
     3001 tacgtccctt atcagcctct tcttcaataa ctaatctgtt tgaagaaaca attgccattt
     3061 ctgatgctgc aaaaaaacca ttaatgaata ttaaaattac taataaaatc aactggagcc
     3121 aaatgtccat ttatctatct tcctttcttt aggtgataaa agtgtatcac attttcatct
     3181 ttttttcaag attattcaaa aagcaaatca attatctcat tacttgtaat taaataaaaa
     3241 taattatcaa ccttttcttc tctaaataac ttacctatat actctttatt atatggttta
     3301 ttaattaata actcttctaa atcactatta gtcttaaccc ccaaaaaatc accataaact
     3361 ttaacattat ttattaaacc atttctaact tcaaaggtta attcgattgt tcccccttta
     3421 aacttagctt ttttcttctt ttgaaaacta aagttcttcc catagttcca ctcccataaa
     3481 ccaaatcttt cgttctttat tttttctatt tcttttaact catcttcatt taaaacatat
     3541 aaatcttctt ttcttaactt taaaactttt gtaatcaaag tatctataac atcttcaatc
     3601 gtgattaaac tattaatctc ttttaaatta actactttgc ttttattgct tttgacagct
     3661 ttatcttcat atttttcttt agaaactaat acttttgata aagtatttaa atcaactgag
     3721 aaaagaatcg ttccatggtg aaacatttta tcttggtaaa aatactgggc attacctgaa
     3781 aacttcgcac catcaatata aatgtcattt ctaccctcaa aattcgcttc tattccaaaa
     3841 tacttcaaag tctcaataat taccatacaa aactttttat agttattaaa tgagtttttt
     3901 tctttagtaa agattgtgta gtttaaattc cctaaatcat gataaactgc accaccacca
     3961 gataaccttc ttgctaaggt aataccattt gcctttaaat actccaaatt aacttcattg
     4021 tatggatttt gatttcttcc aattacgact gtcttatcat tacgccaaag cataataata
     4081 tcttcttcta aatcgtaatt tttgataata tattcttcaa cacttaaatt aaagtgagga
     4141 tctgtatttt gattaataat tactttcata atttactcct tttagtatta tacaatcttt
     4201 ctttaagttt ttcaatttct aagtttattt taagaattca agcgtataat taagctaagg
     4261 tgattcttat gaaagttcta cttttagtct tttcaggtac aaacaactca ttttatgttt
     4321 gtaatgaact aaaaaaagaa tatcaaaagt tgaatattga aactgatatt ttacgtttag
     4381 aaaatgtaaa agaattaaag gacgactatg atttaatagg aatcagttac cctatccatg
     4441 ctttttctgc accaagg
//
LOCUS       DAOSGS010000070         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_17644_length_4454_cov_4.729257, whole genome shotgun sequence.
ACCESSION   DAOSGS010000070 DAOSGS010000000
VERSION     DAOSGS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17644_length_4454_cov_4.729257"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..775
                     /locus_tag="PLQ22_03405"
     CDS             56..775
                     /locus_tag="PLQ22_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH59040.1"
                     /translation="METLKIDKQNTKMIAHRGLSGLEPENTVLAFIAAGNRSYFGIET
                     DVRITKDKKFILCHDDNLKRISGIDVKISEATYEELRSYSLYDFDSKERKEHVRLASL
                     EEYLGICKKYDKVAVIELKEVYREEDVVNLLDTTFQYLKEENVIFISFYLKNLQYIRK
                     YNSNVKIQFLTSDFNKAILNELRKLEAGLDINYHQLSKEIVETCHKYGIEVNVWTVNN
                     PIEAYLLLTWDIDYITSNVLE"
     gene            775..1005
                     /locus_tag="PLQ22_03410"
                     /pseudo
     CDS             775..1005
                     /locus_tag="PLQ22_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            1240..1533
                     /locus_tag="PLQ22_03415"
     CDS             1240..1533
                     /locus_tag="PLQ22_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOH59041.1"
                     /translation="MEKVQTRKEKLADKIFSEEEMKEYQENKSDSYLIKKWTEKEAYF
                     KLLGTGLILNELKQDYSRYVKSYKLEDNKEVYYLSVATNETININILEENIKI"
     gene            1567..2718
                     /locus_tag="PLQ22_03420"
     CDS             1567..2718
                     /locus_tag="PLQ22_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOH59042.1"
                     /translation="MADFKEKVLDLHEKYQGKIEITSKLQINSKEDLALVYSPGVAEA
                     SLAIKENKEDVYKYTSKGKFVAIVTDGSAVLGLGDIGPEAALPVMEGKALLFKKFAGV
                     DAFPICLRTKDVDEIVNTVKLIAPGFGGVNLEDISFPRCVEIEERLKKELDIPVFHDD
                     QHGTAIVTLAALINACKVVGKKLENLKVVISGFGAAGSAIARLLRKANVPSIYAINRK
                     GALSKLKENDPMIDKFLEKGIVDSYDKKDGKLEDIIKDADVFIGVSVADVLTKEMVNM
                     MADKPIVFALANPNPEISSIEVKKTKAYIYATGRSDLPNQINNVLAFPGLFKGVFACK
                     AKQITEEMLLDASQAIASLITKEELSKDYIIPSPFDERVALVVSKAVSK"
     gene            2743..3870
                     /locus_tag="PLQ22_03425"
     CDS             2743..3870
                     /locus_tag="PLQ22_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOH59043.1"
                     /translation="MRYRIEKDKIGKLQLPLEVYYGIHTARSKETFSLTKHGLNRQMI
                     KTLTSIKKASAIANTSLKLLDQKKAEAIILSCDEIFNGRLHGQFVTDLVQGGSGHSMN
                     MNANEVIANRANEMLGGTKGGYEYVSVEDVNLNQSSIGVIVLAGKITAVRLTKKLLNE
                     AKKLSATLKEKIADLKIGEFDYENCLIFMQENLDRNIKKVTKTFDSFKEVAIGMSYFE
                     TKKEIPEEFPKLFVKHLNAAYPSEKVKQASNLIENASDYSCFLELSSALRTLMIDVAK
                     AVDDLKPGIVSGKFVIPQVQESPEPEEPNLIVLEMVNQISFYIMGNDLTIARAVEAGE
                     GVHNIYLPMIYACLFEEINLIRRAIRILREKALVDLEIKEG"
     gene            complement(3891..>4454)
                     /locus_tag="PLQ22_03430"
     CDS             complement(3891..>4454)
                     /locus_tag="PLQ22_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOH59044.1"
                     /translation="EGGPCSMMAGLNDSYDAVIFIGYHSPSRSDGNPLSHTMNTRLHH
                     VKINGEIASEFLINSYYAAWLNVPTVFLSGDLKLTESVKKLFPEAEVVATKEGRHGAV
                     VSRHPNITNQEIEDKVELALKKKKEPANKPISILPKKFKIEIQYKNFNDAYKASFYPG
                     AKLIKDDAIEYETKDYYNALVMMKFLL"
BASE COUNT         1693 a          504 c          781 g         1476 t
ORIGIN      
        1 tttcttttct ttacaaatct cccaattaaa gttataatat accaaggtgg agcttatgga
       61 aactttaaag attgataaac aaaatacaaa gatgatagct catcgcggat taagtggact
      121 tgaacccgaa aatacagttc tagcttttat tgcggctggc aatagaagct attttgggat
      181 tgaaactgat gttaggatca ctaaagataa gaagtttatt ttgtgtcatg atgataatct
      241 taaaaggatt tctggtattg atgttaagat ttctgaggct acttatgaag agttaaggag
      301 ttattcttta tatgatttcg atagcaaaga aagaaaagag catgtaagat tagcttcact
      361 tgaagagtat ttaggtattt gtaagaaata tgataaagtt gcagttattg aattaaaaga
      421 agtttatcgt gaagaagatg ttgtaaattt acttgatact acttttcagt atttaaaaga
      481 agaaaatgta atctttatta gtttttattt aaagaactta caatacattc gcaaatataa
      541 ctcaaatgta aagatccagt ttctaacaag tgattttaat aaagctattt taaatgaatt
      601 aagaaagcta gaagcaggac ttgatatcaa ctatcaccaa ttatctaagg agattgttga
      661 gacgtgtcat aaatatggga ttgaagttaa tgtttggaca gttaataatc caattgaagc
      721 atatttgctt ttaacttggg atattgatta tataacttca aatgttttag aataatgaat
      781 tatgtttata ttttaaggtg cagtgatgat actttatata taggttggac taatgatctt
      841 gaaaaaagaa tcaaagcaca caatgaacaa aaaggagcaa gatacactaa aagaagaact
      901 ccagtggaac ttgtttatta tgaaacattt gaaactaaga ttgaagcttt aaaaagagaa
      961 tttgatttaa aaaagttatc aagagaacat aaactttatt taataaataa tgaagtaaaa
     1021 actaatgttt atgtttttag aagtttaaat caagatgaag atgctaagaa gcttttaaaa
     1081 gaaaaacttc aagaagatta caaattagat atcaaaagtt taaagtttaa atataataaa
     1141 tataaaaaac cttatttaaa cttaccagtt tattttaata ttagtcattc ttatgattta
     1201 atagctttag ttatttctaa atatgaagta ggtattgata ttgagaaagt ccaaacaaga
     1261 aaagaaaaat tagctgataa gatttttagc gaagaagaga tgaaagagta tcaagaaaac
     1321 aaatcagatt cttatttaat taagaaatgg acagaaaaag aagcatactt taagttatta
     1381 ggtactggtt taattttaaa tgagttaaag caagattata gtagatatgt aaagagttat
     1441 aaattagaag ataataagga agtatactat ttatcagttg caacaaacga aacaattaac
     1501 ataaatatac ttgaagaaaa tataaaaatt tgataaaatg aataaacaaa aaaaggataa
     1561 aaggaaatgg cagattttaa agaaaaagtt ttagatttac atgaaaaata tcaaggtaaa
     1621 attgaaataa cttctaagtt acaaatcaat agcaaagaag acttagcttt agtttattcg
     1681 ccaggagtag cagaagcttc tcttgcaatt aaagaaaata aagaagatgt ttataaatat
     1741 acatctaagg gtaagtttgt agctatagta actgatggtt ctgctgtttt aggtttaggt
     1801 gatattggac cagaagcagc cttgccagtt atggaaggta aagctttact ttttaagaag
     1861 tttgctggtg ttgatgcttt tccaatttgt ttaagaacta aagatgttga tgaaatagtt
     1921 aatactgtta aattaattgc tcctggattt gggggagtaa atttagaaga tatatctttt
     1981 ccacgttgtg ttgagattga agaaaggcta aagaaagaat tagatatacc agtttttcac
     2041 gatgatcaac acggaactgc aattgtaact ttagctgctt taattaatgc atgtaaagtt
     2101 gttggtaaaa aactagaaaa cttaaaagta gttatttccg gctttggtgc agcaggttcc
     2161 gcaattgcaa gattattaag aaaagctaat gttccatcta tttatgctat caatcgtaag
     2221 ggtgctttaa gcaaactaaa agaaaatgat cctatgattg ataaattttt ggaaaaggga
     2281 attgttgata gttatgataa gaaagacgga aagttagaag atattattaa agatgccgat
     2341 gtttttattg gtgtatctgt agctgatgtc ctaacaaaag aaatggttaa tatgatggca
     2401 gataaaccaa ttgtctttgc tttggctaat cctaatccag aaataagtag catcgaggtc
     2461 aaaaaaacaa aagcatatat ttacgcaaca ggaaggagcg atttaccaaa tcaaataaat
     2521 aatgttttag cttttccagg actatttaaa ggagtcttcg catgtaaagc aaaacaaatt
     2581 acagaggaaa tgttgttaga tgctagtcaa gctattgctt ctttaattac taaagaagaa
     2641 ctaagtaaag actatattat tccatcacca tttgatgaaa gagtagcttt agtagttagt
     2701 aaggcagtgt ctaaataata aaagatatag gggggataaa ttatgagata tcgtattgag
     2761 aaggataaaa taggtaaact tcagttgcct ttagaagtgt attatgggat tcatactgca
     2821 agaagtaaag aaacattttc cttaacaaaa cacggactaa ataggcaaat gattaaaact
     2881 ctaacaagta ttaaaaaagc atcagctatt gctaatacta gtttaaaact attagatcaa
     2941 aaaaaggctg aagcaattat tttaagttgt gatgagattt ttaatggaag acttcatggt
     3001 cagtttgtaa ctgatttagt tcaaggtggt tctggtcata gtatgaacat gaatgctaac
     3061 gaagtaattg ctaatcgtgc taatgaaatg ttagggggaa ctaagggcgg ttacgaatat
     3121 gtaagtgtag aagatgttaa tttaaatcaa tcaagcatag gggttattgt tttagctggt
     3181 aaaattacag cggttagatt aactaagaaa ttacttaatg aagctaaaaa attaagtgct
     3241 actttaaaag aaaaaatagc tgatttgaaa attggcgaat ttgattatga gaattgtctt
     3301 attttcatgc aagaaaattt agatcgcaat atcaaaaaag taactaaaac ttttgatagt
     3361 tttaaagaag tagcaattgg tatgagttat tttgaaacaa aaaaagaaat tcctgaagaa
     3421 tttcctaaat tgtttgtgaa gcatttaaat gctgcatatc caagtgaaaa agtaaaacaa
     3481 gctagtaact taattgagaa tgcttctgat tattcttgtt tcttagaatt atcatctgct
     3541 ttaagaacat taatgattga tgtagcaaaa gcagtagatg atttaaaacc aggaattgtt
     3601 tcgggtaaat ttgttattcc tcaagttcaa gaaagtcctg aaccagaaga accaaattta
     3661 attgttttag aaatggttaa ccaaatttca ttttatatta tgggtaatga cctaactatt
     3721 gctagagctg tagaagcggg tgaaggagta cataacattt acctaccaat gatctatgct
     3781 tgtttatttg aagagattaa tttaattaga agagcaatta gaattttaag agaaaaagct
     3841 cttgtagatt tagaaataaa agaaggctaa agccttcttt ttaattagta ttataataaa
     3901 aacttcatca ttactaaagc attatagtaa tctttagttt catattcaat cgcatcatct
     3961 ttgattaatt tagctcctgg ataaaatgaa gctttgtatg cgtcattgaa gtttttatat
     4021 tgaatttcaa ttttgaattt tttaggtaaa atacttattg gtttattagc tggttctttc
     4081 tttttcttta aggctaattc aactttatct tcaatttctt gattagttat atttggatgt
     4141 cttgaaacta ctgctccatg acgaccttct ttagtagcta caacctctgc ctcagggaaa
     4201 agttttttta cactttctgt taactttaaa tcgccagata aaaatactgt tggtacattt
     4261 aaccatgcag cataatatga attaattaaa aattctgaag ctatttcacc attaatcttt
     4321 acatgatgta atcttgtatt cattgtatgg cttaagggat taccatcaga tcttgatggg
     4381 ctatgataac caataaaaat aacagcatca taagaatcat ttaatcctgc catcattgag
     4441 catggccccc cttc
//
LOCUS       DAOSGS010000071         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_17767_length_4434_cov_4.488924, whole genome shotgun sequence.
ACCESSION   DAOSGS010000071 DAOSGS010000000
VERSION     DAOSGS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17767_length_4434_cov_4.488924"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..2419)
                     /locus_tag="PLQ22_03435"
     CDS             complement(290..2419)
                     /locus_tag="PLQ22_03435"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HOH59045.1"
                     /translation="MLKLAEYRQQLINDIESDSLTYSEYPEKRFLFFLEDILVNDYSI
                     LSQLDECYFDLPYDKTTNRKGIHIDGGYFEEAANTLHLITIDYNSKDIETLTKDLFDR
                     KVAYLRNYFEAVLNGYFKNGEKSDPAVQFSYSIIENFNRDNIFKINLIIGTTNRFSAG
                     AKLISSLPAIEIKGKTIEVDITSIDIDTIYKTKQVGFKKEPINIHINEFGFEGLPCIK
                     AELFDTNYDAYLVIVPGEFLSNIYKKYNSRLLEDNVRSFLNFRGGINKGIRGTILNEK
                     SNFFAYNNGISTTASKINVVFDNKKGFLITEIENLQIINGGQTTASLAATSIKDKANL
                     EGIFVQMKLTIVQDRDDQFVKNIAKFANSQNKVTSADLNSNHPFYIRMQDFSRKIYTP
                     KAANVSIQTRWYFERSRGQYDQEMLQLSKAQIESFKKQNPPDQKITKTDIAKYLNSDD
                     MKPYDVSWGSDVNATKFYASMEDDWSKNDSKYNELFFKELIGKAILFKHIEKVISNED
                     WYKERNGYRAQLVTYTFSKLVYEASKCKMFIDYMEMWNRQSVPDCFDNDISKIAYLCF
                     ELFYGPLISNISTYCKKKEAWNAVKEMNYNLSSETKDKLITENQKKSQQGSAGKEQRK
                     INVIDNSIYIFSLGVETWQELHKKGKEQKILSYFDEQMIKVAINFCMTGAGCSDKQSK
                     EIIKVIEKLKENGVLIPEGKLFDEESF"
     gene            complement(2413..3363)
                     /locus_tag="PLQ22_03440"
     CDS             complement(2413..3363)
                     /locus_tag="PLQ22_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HOH59046.1"
                     /translation="MIGTMMKINFKDIYENMKFQPFNTQKKLDVDTILSVYYGKTIDN
                     KLRLSFLSKKMPPVIASTRLISIYQVSEENNIYWTCFDLLDIKAATIFYYICDDIVLS
                     IVSEYNEDIALKKIKNRFDLWKKVLNKPINKEMTIEEAKGLFGELYFLNSYMFEKYGI
                     EKAIKSWSGPDGYPKDFSIDNTWYEIKTTSINSNIIKISSLGQLDSEDDGNLVIIRLE
                     NMSEEYDDGLSSLGELLNDIYSKIDDLDLRGMLIEKLMRKGYLIHNSNTSYKFRFVNK
                     TIYLVNDDFPRIKQSDIKYSEVEEVVYSLRISSLERFKKC"
     gene            complement(3338..>4434)
                     /locus_tag="PLQ22_03445"
     CDS             complement(3338..>4434)
                     /locus_tag="PLQ22_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HOH59047.1"
                     /translation="SRGLTLEGLVTSYFSRNGSAYDSMLQMSRWFGYRFNYDDICKIY
                     MTEENLNSFDAINEAVEDLKTQFRRMALAHKTPREFGLMVKERPEALETSLLITARNK
                     MYNAKQIFVTTDFSGYAVDTSKIFKSNEANKTNFNAILKFYNKLFEEGKKFENYSKPL
                     IKNVDKDHIVELIDELEIPMENLKFDQYSLTTYLKESNVLPSWDVVIATGIKVAENEK
                     FNFFEQKLNPVTRSFKYNVGEKYYRISGDKNRLYEPSIFGSGLSDDEIKQVKEKRSMP
                     IAADYLDFPNRHPLLVIYPVKLKTDNQDEMIFISESQYNNAIYGFAIGFPKTSNGIRV
                     KYRANKIKIQEIVGDLDTDDEGYDWDNDED"
BASE COUNT         1437 a          746 c          495 g         1756 t
ORIGIN      
        1 aagttttttt gaagaatcgt aaagttcttc ttttttttat tacattaact tgtatatagt
       61 attttattat tcaacacgat taattaatgt atatgtataa acatttttat aactatacaa
      121 ttttacttta aaatcttcac catcaaatat taatccatat atagtaaaac catatatctc
      181 ctttaaatca tcatttttac aatagactac cataatcgaa agactatata taacgtcgaa
      241 tatagcaaaa aaaagagagt cctattttga ctctcttttt gggcgagttt caaaaactct
      301 cctcgtcaaa taatttgcct tccggaatta gaacaccatt ttcttttaat ttttctataa
      361 ctttaattat ttcttttgat tgtttatcag aacatcctgc tccagtcata cagaaattaa
      421 tagctacttt tatcatttgt tcatcaaaat atgataatat tttttgttct tttccctttt
      481 tatgtaattc ctgccaggtt tccacaccta aactaaaaat gtaaatagag ttatctatta
      541 cgtttatttt tctttgttct tttcccgctg agccttgttg agatttcttt tgattttcgg
      601 taatcaattt gtcttttgtt tcactagaaa gattataatt catttcttta accgcgttcc
      661 atgcttcttt tttcttacaa taagtactaa tatttgaaat taatggacca tagaataatt
      721 caaaacataa ataagctatt ttactaatat cattatcgaa acaatccgga actgattgtc
      781 tattccacat ctccatataa tcaataaaca ttttacactt gcttgcttca tagaccaatt
      841 tactaaatgt atatgttacg agttgagcac gataaccatt tctttcttta taccaatctt
      901 cgttggatat tactttctct atatgcttaa ataaaatcgc tttaccgatt aattccttaa
      961 agaataattc attatatttt gaatcattct ttgaccaatc atcttccata gaggcataga
     1021 attttgtggc atttacatca gatccccatg aaacatcata tggtttcatg tcatcagaat
     1081 ttaaatattt agctatatca gtctttgtta ttttttgatc aggtggattc tgttttttaa
     1141 aactttcgat ttgtgctttt gataattgaa gcatttcttg atcatattgt ccccttgatc
     1201 tttcaaagta ccatctagtt tgaatagaaa cattagctgc cttaggagta taaatttttc
     1261 tactgaaatc ttgcattctt atatagaatg ggtgatttga atttagatct gcagaagtta
     1321 ctttattttg gctattagca aatttagcga tgttctttac aaactgatca tccctatctt
     1381 gaactatagt caacttcatt tgtacaaaaa taccctcaag atttgcttta tctttaatcg
     1441 atgttgctgc taatgatgct gtagtttgac caccatttat tatttgcaag ttttcgattt
     1501 ctgtaattaa aaagcctttt ttgttatcga aaactacatt aatttttgat gcggtcgtag
     1561 aaataccatt attataagca aagaaatttg atttctcatt aagtatcgtt cctcttattc
     1621 ctttgtttat tccgcctcta aaatttaaaa acgatctaac attatcttcc aataatcttg
     1681 aattatattt tttgtaaata ttagataaga attctccagg aacaataaca agataagcat
     1741 catagtttgt gtcaaatagc tctgctttaa tacatggcaa tccttcgaaa ccaaattcgt
     1801 ttatatgtat atttattggc tcttttttaa atccgacttg ctttgtttta taaatggtat
     1861 caatatcaat agaagtaata tcaacttcta ttgtttttcc ttttatctct atagcgggta
     1921 aagatgaaat aagtttagct ccagcagaaa acctattggt cgttcctata attaaattaa
     1981 ttttaaaaat gttgtctcta ttaaaatttt caattataga ataagaaaac tgtacagctg
     2041 gatcactttt ttcaccattt ttaaaatatc cattaagaac agcttcaaaa taatttctca
     2101 gatacgctac ttttctatca aacaaatctt ttgtcaatgt ttcaatatct ttactattat
     2161 aatcaatagt aataagatgc aatgtatttg cagcctcttc aaagtatccg ccatcaatat
     2221 gaattccctt acgatttgtt gttttatcat aaggaagatc gaaataacat tcatccaatt
     2281 gactcaaaat gctataatca tttactaata tatcctctag aaagaataaa aatctttttt
     2341 ctggatattc agaataagta agcgaatcag attcaatatc attaatcaat tgttgtctat
     2401 attcagcaag ttttagcatt ttttaaacct ctctaatgaa gaaatcctta atgaataaac
     2461 aacttcctct acttcgctat attttatgtc tgattgctta attcttggaa aatcatcatt
     2521 aactaaataa attgttttat taacaaatct aaatttataa ctagtattcg aattgtgaat
     2581 cagatatcct ttgcgcatca atttttcaat tagcattccc ctcaaatcaa gatcatctat
     2641 tttcgagtaa atgtcattaa gcaactcacc aagagatgat aaaccatcat catattcttc
     2701 agacatgttt tccaatctaa taattactaa gttgccatca tcttctgaat caagttgccc
     2761 caaagaacta atcttaatga tatttgaatt gatagatgta gttttaattt cataccatgt
     2821 attatcaata gaaaaatctt ttggatagcc atcaggacca gaccaagatt taattgcttt
     2881 ctcaattcca tatttttcaa acatatatga attaagaaaa tatagttcac caaataaacc
     2941 ttttgcttct tctattgtca tttctttatt tattggtttg tttaacactt ttttccacaa
     3001 atcaaatcta ttctttatct ttttaagagc tatgtcttca ttatattcag atacaatgct
     3061 taaaactata tcatcacata tataataaaa tatagtagct gctttaatat ctaataaatc
     3121 gaagcaagtc caatatatat tgttttcttc gcttacttga tatatgctaa ttaatcttgt
     3181 tgaagcgata actggcggca tttttttaga taaaaatgat agtctcaatt tgttatcaat
     3241 tgtttttcca taatacacac tcagtattgt atcaacatct aatttttttt gagtattaaa
     3301 tggctgaaac ttcatatttt catatatgtc tttaaaatta atcttcatca ttgtcccaat
     3361 catacccttc atcgtcagtg tctaagtcgc caactatttc ttgaattttt atcttatttg
     3421 ctctatattt tactctaatt ccgttagatg tcttcggaaa accaatggca aatccatata
     3481 ttgcattatt atattgagat tcgctaataa aaatcatttc atcttgatta tcagttttta
     3541 atttaactgg ataaataacc agtagcggat gtctatttgg aaaatctaag taatcagcag
     3601 caataggcat agatcttttt tcctttacct gcttaatctc atcatctgat aaaccactac
     3661 caaatattga tggctcatat agcctatttt tatcgcctga tattctataa tatttttcac
     3721 ctacattata cttaaagcta cgagtaactg gatttaattt ttgttcaaaa aaattaaatt
     3781 tttcattctc agctactttt ataccagtag caataactac atcccaagag ggtaaaacgt
     3841 tagattcttt taaatatgtt gtcaaactat attgatcaaa ttttaaattt tccatcggga
     3901 tttctaattc atcaatcaat tcaactatat gatctttatc tacattttta ataagaggtt
     3961 ttgaataatt ttcaaatttc ttaccttctt caaacaattt attataaaac tttaaaatag
     4021 cattaaaatt tgttttattt gcctcatttg atttaaatat tttcgaggtg tcaacagcat
     4081 aaccagaaaa atcagtagta acaaatattt gttttgcatt atacatctta tttctagcag
     4141 ttattaatag tgatgtttct aaagcttctg gacgttcttt taccattagt ccgaattcgc
     4201 gtggcgtttt atgtgccaaa gccattcttc taaattgtgt ttttaaatcc tctactgctt
     4261 cattaatcgc atcaaatgaa ttcaaatttt cttctgtcat atatattttg catatatcat
     4321 cataattaaa cctgtaacca aaccatcgcg acatttgaag catcgagtca taagcactac
     4381 catttctact aaaataactt gtgactaagc cttctaatgt aagacctctt gata
//
LOCUS       DAOSGS010000072         4429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_17795_length_4429_cov_4.579332, whole genome shotgun sequence.
ACCESSION   DAOSGS010000072 DAOSGS010000000
VERSION     DAOSGS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17795_length_4429_cov_4.579332"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..271
                     /locus_tag="PLQ22_03450"
     CDS             122..271
                     /locus_tag="PLQ22_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59048.1"
                     /translation="MAHLGISIKDAKYIDIDVYVELINLELETISKEDVPRKATQKDI
                     DLFLL"
     gene            308..658
                     /locus_tag="PLQ22_03455"
     CDS             308..658
                     /locus_tag="PLQ22_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59049.1"
                     /translation="MSKKEKPKFEPCINISIEPCWKCKEEMRVAYYSDNLQMPYGPSN
                     FTDKQIEIGRSAGCKIDLAFSKTMQEQYLANICKNCGAFKGDFFYHDYAYVPGDIQYF
                     LDEDDNIVKVVNNE"
     gene            651..1040
                     /locus_tag="PLQ22_03460"
     CDS             651..1040
                     /locus_tag="PLQ22_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59050.1"
                     /translation="MNNYQKGRNFELKAKAYFENLYGVEFKSGKILTGLNGGKPRQFD
                     LINEENKILIECKNFIFTETGNLPSAKISDFLKEIYKLHLAPNDYKKIMCIAYAYSDT
                     KKITLKEYIVNQYFDFIPDTIEIVELK"
     gene            1087..2313
                     /locus_tag="PLQ22_03465"
     CDS             1087..2313
                     /locus_tag="PLQ22_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59051.1"
                     /translation="MAETVKGINIKLSLDGKDLENEIKGINKDLKEQQKDLRAINANL
                     KYDSSNLELWKKKQGQLNEILKSTKERLEKQNEQLVKAKEGLKLGTISDAEFKKLERN
                     IAYTEADLRRVNSELDKTKDKMKSLGNEKFENLTKLGGTLTKSLTMPILGAVTALTAL
                     ATKGINTADELKNTAQKIGMNVEALQEWNHVAKLAGVETSSLEKAFSKVNNILADVAL
                     GDVKSFAGVFHALGISMDEIEGKTTEEAFDIIREALNKVEDQALRTALANNLFGDKLG
                     SELIPILGLEQDEIARLRNEARLLGLITEEQIEQTGGYKDSLDRLKQSTTALSVEIAS
                     VMIPAMSKIVSILQDKVIPAVKSAVEWWQNLDDRTKAIIVALTGLVAVKDNAVSILKG
                     LSFKYISFRFESKVAI"
     gene            complement(2790..2990)
                     /locus_tag="PLQ22_03470"
     CDS             complement(2790..2990)
                     /locus_tag="PLQ22_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH59052.1"
                     /translation="MVYNYNGLWKMLIDKNLKKSDLMKKVGIASSTMAKMGKGLPVKL
                     EVLARICREFDCNIGEILRIDY"
     gene            3371..3880
                     /locus_tag="PLQ22_03475"
     CDS             3371..3880
                     /locus_tag="PLQ22_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59053.1"
                     /translation="MPKVYRDTKYYDLEELKKKIAEGLYDEFGNKLTNWVSYEDRDKK
                     YVYVPCKEDFFHWYRNENRNEERRNFREKERCPISIDQMREDHDFEYADNSYYENQER
                     ERAQEVSDLIWSLVSEFDPIDQEIIRLYNEGHTDSYISQQVNMPRSTIQWRKRMVIEL
                     LKEKMTKIR"
     gene            3959..4135
                     /locus_tag="PLQ22_03480"
     CDS             3959..4135
                     /locus_tag="PLQ22_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59054.1"
                     /translation="MEKESKADKPGITNKDLVESLLLISEASKTLALEIMLLPKNEEV
                     EGGGENGTEPNNSQ"
     gene            4107..>4429
                     /locus_tag="PLQ22_03485"
     CDS             4107..>4429
                     /locus_tag="PLQ22_03485"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HOH59055.1"
                     /translation="MALSPTTHSRKYSPSKSGTWLNCPLSTLLNDGSNQEVSPQAEFG
                     TQCHELGSALISKSLKLIDYDSEIKPIDDLIKDLDMYSPDMQEIADGYADFVISTFDY
                     EKNKSG"
BASE COUNT         1718 a          627 c          767 g         1317 t
ORIGIN      
        1 atgttggtga acttgaaaac atttcacata ccattgcaga attactaggc ggaagtgtaa
       61 acaacgaaag cccaaaatag aatctcaaga tgagcacgtc acagcaaata taatttataa
      121 tttggctcat cttgggattt caattaaaga tgctaaatac atagatattg atgtatatgt
      181 agaattaatt aatttagaac ttgaaactat atcaaaagaa gatgtaccaa gaaaagcaac
      241 tcaaaaagat atagacttat ttttactcta atatcgtgtt ataattcctt taagggagtg
      301 atgtaaattg agtaaaaaag aaaaaccaaa atttgaacca tgtattaaca tttcaattga
      361 gccttgttgg aaatgtaaag aagaaatgag agtagcatat tattctgata atcttcagat
      421 gccatatggg ccaagcaact ttacagataa acaaatagaa ataggcagat ctgcaggttg
      481 taaaattgat ctagcattta gtaaaacaat gcaagaacaa tacttagcaa acatatgtaa
      541 aaattgtggt gcatttaaag gagacttttt ctatcacgat tatgcatatg tcccaggtga
      601 tattcaatat tttttagatg aagatgataa tattgtcaag gtagttaata atgaataatt
      661 atcagaaggg tagaaatttt gagcttaaag ctaaagctta ctttgagaac ctatatggag
      721 ttgaatttaa aagtggtaaa attttaacag gtttaaatgg tggaaaacca agacagttcg
      781 acttgataaa tgaagaaaat aaaattttga tagaatgtaa gaacttcata tttactgaaa
      841 caggaaactt accttccgct aaaatatccg acttcttaaa agaaatttac aaattacatt
      901 tagctccaaa tgattataaa aaaataatgt gtatcgctta tgcttattcg gacactaaaa
      961 aaataactct aaaggaatac atagtaaatc aatactttga cttcattccg gacacaattg
     1021 aaatagtaga attgaaatag aaagggcgga agtccttttt ttattaccaa aaggaggtga
     1081 gtgttaatgg cagaaacagt taaaggtatc aacataaaac ttagcttaga tggtaaggat
     1141 ttagaaaatg aaataaaggg tattaataag gatctaaaag aacaacaaaa agatctaaga
     1201 gcgattaacg caaacctaaa atatgatagt tcaaatttag agctttggaa aaagaaacaa
     1261 ggccaattaa acgaaatctt aaaaagcact aaagaaagat tagaaaaaca aaacgaacaa
     1321 ttagtaaaag ctaaagaagg attaaagctt ggcactatat cagatgctga gtttaagaag
     1381 ttagaaagaa acatcgctta tacagaagca gacctaagaa gagttaatag tgagcttgat
     1441 aaaactaaag ataaaatgaa gtctttaggt aatgaaaagt ttgagaattt aactaaactt
     1501 ggtggtacat taactaagtc attaacaatg ccaattttag gagcagttac tgcacttact
     1561 gcacttgcta ctaaaggtat taatacagca gatgaattaa agaacactgc tcaaaagata
     1621 ggcatgaatg ttgaagcctt acaagagtgg aaccatgtgg ccaaacttgc tggagttgaa
     1681 acttcaagtt tagaaaaggc attctcaaaa gttaataaca tattagctga tgttgcactt
     1741 ggtgatgtta aatcatttgc tggagtattt catgctcttg gtatttcaat ggatgaaatt
     1801 gaaggtaaaa ccacagaaga agcatttgat atcattagag aagctttaaa taaagtagag
     1861 gatcaagcac taagaacagc ccttgctaat aatttgtttg gcgataagtt aggtagtgaa
     1921 ttaataccta tcttaggatt agaacaagac gaaatagcaa gactaagaaa cgaagcaaga
     1981 ttactcggtt taattacaga agaacaaatc gaacaaacag gtggatataa ggattcgctt
     2041 gatagattaa aacaatcaac aaccgctcta tcagtggaaa ttgcatcagt tatgatacca
     2101 gcaatgagta agattgtaag catccttcaa gacaaagtta taccagctgt taaaagtgca
     2161 gtcgaatggt ggcaaaacct agatgataga actaaagcaa ttattgttgc actaacagga
     2221 cttgttgctg taaaagataa tgcagtatct attcttaaag gcttatcatt taaatatatt
     2281 tcatttcgct ttgaatcaaa agtagcaata taactagaat ctatctcagg agtatttata
     2341 tttgggattt tcatacaata aatagttaca tttgctaata agtctgaaaa tgagaaagtt
     2401 gtattattaa taattgaatg ttttgtatag tatttattgt cacctatgat tgttgaatct
     2461 ggcaacgatg attcggagta tagcaaatct ttaatagcca aaaccacgtt tgctttactt
     2521 gttggtgaaa tgtttttttc caatccatca acatacaccc tacttaaatc gtcaacattt
     2581 tgttttgaag cctcaacaga ataagaacta ggtagattaa gtgctccagt tttatatcta
     2641 gaaatatcac ctttatcaat tctatatcca tgtttaaaaa atggtcctac gaccaaatca
     2701 aaaagaactt catcatttat ctttattttt gattgagata aaactttaaa ataagaacta
     2761 aaacataatt ttttcatagg cgattctcct taataatcaa ttcgcaatat ttctccaata
     2821 ttacaatcga attctctaca aattctcgca aggacttcca atttaactgg cagtcctttt
     2881 cccatttttg ccattgttga agaagctatg cctacctttt tcattaaatc acttttttta
     2941 agatttttat caattaacat tttccataaa ccattataat tgtataccat aaaattcaca
     3001 ctcctatcac tttaattata acacatctaa atatttattt ttactctata aagaataaat
     3061 ttgcgtttac aaattttttg ttttccaaca aatttccaac aaaaacacaa caatctacat
     3121 atctacaaaa aaataaaggt ttgataaact acaagtgtat taagaaatat tatcttaatt
     3181 agtgctttac aacactgatc aagttgtcgc aataaagcac ataaatttaa taaagcagtt
     3241 aaatctattg cgaggaatag gctgcaattg aaatggaaat tcacttccgt tacttttgcc
     3301 acgcctcaaa tgttttaact gcaaagtgga cctccatttc aaagagaaat tgaaaagggg
     3361 gttctttttt atgcctaaag tttatagaga tacaaaatat tacgatttag aagagttaaa
     3421 gaaaaaaatt gcagaaggct tatatgatga atttggtaat aagctaacga attgggtctc
     3481 atatgaagat agagataaaa agtatgtata tgttccttgt aaagaggatt tttttcattg
     3541 gtataggaat gaaaacagaa atgaagagag aagaaacttt agagaaaagg aaagatgtcc
     3601 aatctctata gatcaaatga gagaagatca tgattttgaa tatgctgata attcttacta
     3661 tgaaaatcag gaaagagaaa gagcacaaga agttagtgat ttgatttggt ctttagtttc
     3721 tgagttcgat ccaatagatc aagaaatcat cagactttat aacgaaggcc atactgattc
     3781 ttatattagc caacaagtaa atatgccaag aagcacaatt caatggcgaa agcgaatggt
     3841 aattgaactt ttaaaagaaa aaatgaccaa aattcgctaa atcttggcaa aacgataatc
     3901 gacttgccat taaccattga agggaaagaa aactcttcag gaagaaggag gtttagaaat
     3961 ggaaaaagaa tctaaagccg ataaacctgg tatcaccaat aaggatttag tagagtccct
     4021 acttcttatt agtgaagcca gtaaaacatt ggcgctagaa attatgttgc ttccaaaaaa
     4081 tgaggaagta gaagggggtg gtgagaatgg cactgagccc aacaactcac agtagaaaat
     4141 atagtcccag caaaagtggt acttggttga attgcccact tagcacccta ttaaatgatg
     4201 gaagcaatca ggaagtaagt ccacaagcag agtttggaac acaatgtcat gaattaggct
     4261 cggcactaat tagcaaatca ttaaagctta ttgattatga cagtgagatt aaaccaatcg
     4321 atgatcttat taaggatctg gacatgtact cgcctgatat gcaggagatt gcagatggtt
     4381 atgctgactt tgttattagt acttttgatt atgaaaagaa caagtctgg
//
LOCUS       DAOSGS010000073         4389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_18005_length_4389_cov_4.202584, whole genome shotgun sequence.
ACCESSION   DAOSGS010000073 DAOSGS010000000
VERSION     DAOSGS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18005_length_4389_cov_4.202584"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLQ22_03490"
     CDS             complement(<1..838)
                     /locus_tag="PLQ22_03490"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HOH59056.1"
                     /translation="MKVRNKKWTEEEFLKVREEVLKSWKTGSSPLLDFEVSIPYLKSL
                     PKKKNMAYVLNEAKSKGRTLVQPRAGVASLDKHIELLQKLEKAGADALPSTIDSYTRL
                     NRYEEAERGLEESNRVGKSMLNGFPAVNHGVEACRKVVESVNVPVQARHGTPDSRLLA
                     EIVHAAGFTSNEGGAISYNLPYAKSVSLEDTIYYWQYCDRLVGYYEDHGVRLNREPFG
                     PLTGTLVPPCITNVIGIIEALLAAEQGVKNITVGYGEGGNFSQDLAAINALQDQTEYY
                     LYK"
     gene            complement(835..2211)
                     /gene="glmL"
                     /locus_tag="PLQ22_03495"
     CDS             complement(835..2211)
                     /gene="glmL"
                     /locus_tag="PLQ22_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HOH59057.1"
                     /translation="MKYLLLDFGSTYTKLTAVDISKAKVIGHTDAITTINTDIKDGYH
                     ECLKKLEAKVGKQKYDKIIACSSAAGGLKMAAIGLVESLTVEAARRVCLGAGAKVDLV
                     VSGRINKSQMEVITSGKIDIVLLAGGTDGGDRDTVLENAKRLAQAKVKVPIIYAGNKE
                     CQDDISEILSEANIKPYTCENVMPLLNQLNIKNTQEVIREIFMGNIIKAKGIGKIEEE
                     IDEVIFPTPKAVLEAATLLADGYLDEEGLGDIILVDVGGATTDIYSIASGAPSKVSVL
                     QRGLEEPYAKRTVEGDLGMRYSALGILEALSLEERKKYLKDGINIEKEAKKRHDNIEF
                     LPATDYDFKVEKEMAKVALDNAISRHVGFLESVYTPFGVMEYQTGKDLTKVKFVIGTG
                     GVLINSKDTKDILSSITYTLKKPNELRPLKSEFMVDKSYILSAMGLLSIDYPEVALKI
                     MKEEIVKL"
     gene            complement(2208..2636)
                     /gene="glmS"
                     /locus_tag="PLQ22_03500"
     CDS             complement(2208..2636)
                     /gene="glmS"
                     /locus_tag="PLQ22_03500"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HOH59058.1"
                     /translation="MKKPTIVIGVIGADVHAVGNKIIEYILKENGFNVANVGVLSSQE
                     DFINAAIETNAPMILVSSLYGQGELDCRGMRDKCIEAGIPDVILYVGGNIVVGKQDFK
                     DVEKRFKDMGFNRVFPPGTPFEDTLVWVIEDLKALEAKGK"
     gene            complement(2764..>4389)
                     /locus_tag="PLQ22_03505"
     CDS             complement(2764..>4389)
                     /locus_tag="PLQ22_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH59059.1"
                     /translation="ESFLSSVSITRNKDDQTKANLSFNTSYYGNIGHVKVEDKTGRVY
                     LDTVLKDETMTSFLFSGLQLDDVKDFVVKFKKYDGTVITKDIHFINSLYYGRIYDLRF
                     SDQGERNYSIIFREELDGNVLDKYEIYFDDACQAEYPVGTRRINITSVPADVREIILK
                     LKAADGTILDEYYFDVALPVALSVGDESYDVNVGKTIEIEVSQVPNHGHELIVKSESD
                     NYSYEIVGNVLKITGINVATENVTVGLVDNDAEVTFALYILDATPGELLDFAAIRNNQ
                     NIEVSFTEDINPELVKEYKVYFNDALKTTLQKGANSYSFKETKAGTVKVEIIDKDNNV
                     VKSYTSQVGKFTELSLAPATKDIYKGDEIEVVITSHTDNLSVEGLSGLQISAITNKKF
                     TVKGLAYGNYEVKVKNQDTNETATLTLKVKPKTINVVIGGLEGKETITINEKVNLVVE
                     VYLDGELATLKPGDVVFSSNNQSVATISNTGVIEALQAGDVKISVQYGDITKTYNLEV
                     LKKKGCNSQAIALVALLPFAFLGFRRREDYFEE"
BASE COUNT         1405 a          891 c          472 g         1621 t
ORIGIN      
        1 atttatataa ataatattct gtttggtctt gtaaagcatt aattgcagct aagtcttgag
       61 agaagttccc tccttcgcca taaccaacag taatgttttt aactccttgt tcagctgcta
      121 aaagtgcttc aattattcca attacgttag taatacaagg aggaactaaa gttccagtta
      181 atggtccaaa aggttcccta tttaacctaa caccatgatc ttcatagtaa cctactaagc
      241 gatcacaata ttgccagtag taaattgtat cttctaatga tactgactta gcataaggta
      301 aattataaga aatagctcca ccttcattag aagtaaagcc agctgcatga acaatctccg
      361 ctagaagtct tgaatctggt gttccatgtc ttgcttgaac aggaacattt acactttcta
      421 caaccttcct acaagcttca actccatgat ttacagccgg aaagccatta agcatactct
      481 taccaacacg attactttct tctaaaccac gttctgcttc ttcataacga ttaagtctag
      541 tgtaagagtc gattgtagaa ggaagagcat cagctcctgc tttctctaat ttttgtaata
      601 actcgatatg tttatctaag ctagctacac ctgctctagg ttgaactaaa gttctacctt
      661 tactcttagc ctcatttaaa acataagcca tattcttctt ctttggcaga ctctttaaat
      721 aaggaattga aacctcaaaa tctaaaagtg gagatgaacc agtcttccac gactttaata
      781 cttcttctct aacttttaaa aattcttctt cagtccattt tttatttctt actttcataa
      841 cttcacaatt tcttctttca taatttttaa agctacttct ggataatcaa tactcaatag
      901 tcccatagct gataagatat aacttttatc gaccataaac tcactcttta atggtcttaa
      961 ttcgtttggt ttttttaaag tataagtaat agaacttaaa atatctttcg tatctttgct
     1021 attaattaat actccaccag taccaataac aaactttact ttagttaaat cttttccagt
     1081 ttgatattcc attactccaa atggagtata aacagattct aagaaaccta catgtcttga
     1141 gattgcatta tctaaagcaa ccttagccat ttctttttcc actttaaaat cataatcagt
     1201 tgctggtaaa aactcaatat tatcatgtct ttttttagct tctttttcaa tattaattcc
     1261 atcttttaaa tatttctttc gctcttctaa acttaaagct tctaaaatac ctaaagcaga
     1321 atatctcatc cctaaatccc cttctactgt tctcttggca taaggttctt ctaatcctct
     1381 ttgtaagaca gaaactttag atggtgcacc tgaagcaata gaataaatat ctgtggtagc
     1441 tccacctaca tcaactaaaa taatatctcc taaaccttct tcatctaaat atccatcagc
     1501 taaaagcgta gccgcttcta aaacagcttt tggcgtaggg aaaataactt catctatttc
     1561 ttcttcaatt ttccctattc ctttagcttt aataatatta cccataaaga tttcacgaat
     1621 aacttcttga gtatttttaa tatttaattg gttaagtaaa ggcattacat tctcacaagt
     1681 ataaggttta atattagctt cacttaaaat ctcactaata tcatcttggc attctttatt
     1741 acctgcataa ataattggaa ctttgacctt tgcttgagca agccttttgg cattctcaag
     1801 gactgtatca cggtctccac catcagttcc ccctgcaagt aaaacaatat caattttacc
     1861 tgaagtaata acttccattt gacttttatt gattctacct gaaacaacta aatcaacttt
     1921 agctcctgcg ccaagacaaa ctcttcttgc tgcttctact gtcaaacttt caactaaacc
     1981 aattgctgcc atctttaatc caccagcagc tgaagaacaa gcaataatct tatcatattt
     2041 ttgcttacca actttagctt ctaacttttt taaacattca tgataaccat ctttaatatc
     2101 agtattaatt gtagttatag catctgtatg gccaataact ttagctttac ttatatcaac
     2161 agcagttaat ttagtataag tagatccaaa atctaataat aaatatttca ttttcctttt
     2221 gcttctaaag cctttagatc ctctattacc caaactaatg tatcttcaaa aggtgttcct
     2281 ggtggaaaaa cacgattaaa tcccatatct ttaaatcttt tctctacatc tttaaaatct
     2341 tgtttcccaa caacaatatt tccacctaca tataaaataa catcaggaat accagcttca
     2401 atacacttat ctctcatccc tctacaatca agttctcctt gaccataaag tgatgatact
     2461 aatatcatcg gtgcattagt ttctatagct gcattgataa agtcttcttg actagaaagc
     2521 acacctacat tagctacatt aaatccattt tcctttaaaa tgtattcgat aattttatta
     2581 cctactgcgt gtacatcggc accaataacg ccaataacta ttgttggttt cttcattatt
     2641 tttcttcctc atttcttcta aaatactact agtattatac tacttttacc ttgtttcttc
     2701 aagttataaa taaaaagcaa tacctttaaa aagtattgct tttttatatt ttattaacct
     2761 atattattct tcaaaataat cttctcttct tctaaaaccc aaaaaggcaa aaggcaataa
     2821 agcaactaaa gcaattgctt gactattaca accttttttc tttaatactt caagattata
     2881 agttttagta atgtctccat attgtactga aatcttaaca tctcctgctt gtaatgcttc
     2941 aataactcca gtattagaga ttgtggcaac actttgatta ttagatgaaa atactacatc
     3001 tcctggtttt aaagtagcaa gttctccatc taaatatacc tctacaacca aattaacctt
     3061 ttcattaatt gtaattgttt ctttaccttc taaaccacca ataacaacat taatagtttt
     3121 tggttttact tttagtgtaa gagttgcagt ctcattagta tcttgattct ttactttaac
     3181 ttcataattt ccataagcta aacctttaac tgtaaacttc ttatttgtaa ttgcactaat
     3241 ttgtaaacca cttaatccct caacacttaa attatcagta tgagatgtaa tcacaacttc
     3301 tatttcatca cctttataga tgtcttttgt tgcaggggct aaactcaact cagtaaactt
     3361 acctacttga gaagtataac ttttaactac attattatct ttatcaataa tctcaacttt
     3421 aacagttcct gcttttgttt ctttaaaact atatgaattt gcaccttttt gtaaagtagt
     3481 ttttaatgca tcattaaagt aaactttata ttcttttact aattcaggat taatatcttc
     3541 agtaaatgaa acttcaatat tttgattatt tcttatagct gcaaaatcta acaattcgcc
     3601 tggcgtagca tctaaaatat ataaagcaaa tgtcacttct gcgtcattat caactagacc
     3661 tacagttaca ttttctgttg ctacattaat acctgtaatc tttaatacat tacctacaat
     3721 ctcataacta taattgtcac tttcagactt tacaattaac tcatgaccat ggtttggaac
     3781 ttgacttact tcaatttcaa ttgtcttacc aacatttaca tcatagcttt catcacccac
     3841 acttaaagcc acaggtaaag ctacatcaaa ataatactca tctagtattg ttccatctgc
     3901 agcttttagt tttaaaataa tttctcttac gtcagctgga acagatgtta tattaattct
     3961 tcttgtgcct actggatatt ctgcttgaca agcatcatca aaataaatct catatttatc
     4021 taagacattc ccatctaact cttctctaaa aataatcgaa tagtttctct ctccttgatc
     4081 actaaatcta agatcataaa ttcttccata atataaagag ttaataaagt gaatatcttt
     4141 tgtaattact gttccgtcat atttcttaaa cttaactaca aagtccttta catcatctaa
     4201 ttgtaaccca ctaaatagaa aactagtcat tgtttcatct tttagtactg tatctaaata
     4261 tactctacca gttttatctt ccacctttac atgacctata ttgccataat aagaagtatt
     4321 aaaacttaag ttagctttag tttggtcatc tttgtttctt gttattgaaa ctgaacttaa
     4381 gaatgactc
//
LOCUS       DAOSGS010000074         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_18669_length_4274_cov_3.740223, whole genome shotgun sequence.
ACCESSION   DAOSGS010000074 DAOSGS010000000
VERSION     DAOSGS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18669_length_4274_cov_3.740223"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLQ22_03510"
     CDS             <1..420
                     /locus_tag="PLQ22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOH59060.1"
                     /translation="LAARGMDITGVSLVVNYSLPTDIKYYIHRSGRTARFKDTGKVIS
                     LLDFDDEKYLARLHGRGLDIYFGEIIEGMFKETKYQERKKNKYDEEIEVLHVKYPLPP
                     KVKPNYRKRRKEKIAKETRQIKRKRVEDIYKRKAKND"
     gene            413..1282
                     /locus_tag="PLQ22_03515"
     CDS             413..1282
                     /locus_tag="PLQ22_03515"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOH59061.1"
                     /translation="MIKIGSHVSNKGDEMLVGSVLEAVSYDANCFMVYLGAPQNSYRK
                     DFESFRKDEFQHLLKEHKINIDDLIVHAPYIINMANPDLEKREFAIDFLTRELLLMEN
                     LGFTKMVVHPGNSLGDDNAINYLKESLIKILDNTKNTNTMILLETMAGKGTEIGRNFE
                     ELAFLLEGLPEERVNVCFDTCHTFDSGYDTFNDYEGVLEKFDEVIGLERIKAIHLNDS
                     KNPLGSQKDRHENIGYGYIGFETLLKFVYDPRFKDIPKVLETPYIDGKAPYKEEIEMI
                     KMKKFNPKLKGES"
     gene            complement(1279..2334)
                     /gene="ispG"
                     /locus_tag="PLQ22_03520"
                     /gene_synonym="gcpE"
     CDS             complement(1279..2334)
                     /gene="ispG"
                     /locus_tag="PLQ22_03520"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOH59062.1"
                     /translation="MRKTKKIFIGNKEIGGNSPILIQSMTNTKTSDINATVSQILALE
                     KAGCELIRCAITSKEDALALKEIKKQIHIPLAADIQFDYKLALLAIENGADKIRINPG
                     NIGTKEEIGEIVEKCKKHNIPIRIGINSGSIEKDLAEKFGNSKEALVESAIRNIRILE
                     SFHFHNIILSVKSTNLEATIEAYKVLNANYSYPLHIGITESGPKQMGTIKSSIGLGIL
                     LYEGIGDTIRVSLTADPVEEISVAKDILASLGLYNKPVLISCPTCGRTEYNMMEITKE
                     IESFLNTLGNVKLTVAIMGCAVNGPAEAKDADIGIAGGKGEALLFKKGQAIRKIKAEN
                     IVKELKNEINKMIKGEF"
     gene            complement(2321..2788)
                     /locus_tag="PLQ22_03525"
     CDS             complement(2321..2788)
                     /locus_tag="PLQ22_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59063.1"
                     /translation="MSKYNVFTSTIGDCTVVEIKPRKLTKEETKKLRAIRRTIMGEYN
                     GPRAARYLKISTRQVRNLKSAVYAYGDYGVIHKNRFHRPPIAYSEEVRKEIVDLYRKK
                     ENRGMLISHFAEMLIDEFGYDMAPSTIYNILRENRIRSPKRHKKKRKTNNEKN"
     gene            complement(2829..2984)
                     /gene="rpmG"
                     /locus_tag="PLQ22_03530"
     CDS             complement(2829..2984)
                     /gene="rpmG"
                     /locus_tag="PLQ22_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH59064.1"
                     /translation="MRVTFILRCTVCGEENYLSEKNKKNTPDRLEVKKYCPRCRQQTL
                     HKEKTKK"
     gene            complement(3046..>4274)
                     /locus_tag="PLQ22_03535"
     CDS             complement(3046..>4274)
                     /locus_tag="PLQ22_03535"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOH59065.1"
                     /translation="WIMEPLASKIRPKKFSNIYGQDHLIGTNGPIFRMVKNNKLQSLV
                     LYGEPGIGKTTLAIVIANELNRPYQLFNASNDNKATLKEIIDNHSDSILIIDEIHRMK
                     KDIQDYLLPHLEDGSITIIGLTTINPYHSLNPPVRSRTLIYRLKPLQYQDLEKIFYDG
                     LEHLESHHKFSNDAKKYLISCANGDVRSLLNYLEATVNTIDTEQVSLDDVQRTIQIAS
                     VSIDKDGENYYDTLSGLQKSIRGSDVNAALHYLARLLMADDLLPLIRRLYAICYEDIG
                     LANPGLGPKVHAACEIALDLGMPEAKLPLSVIVIDLALSPKSNSAYLAVSKAIEDVEK
                     GKTGPIPLNLKNTYSFDPSQKSYLYPHDYPGAWVKQQYLPDIIKDAKYYDPKETGKYE
                     KALKERLEAIEKAKNN"
BASE COUNT         1393 a          646 c          670 g         1565 t
ORIGIN      
        1 cttgctgcgc gtggtatgga tattacggga gttagtttag ttgtaaatta ttcgttacct
       61 accgatatta aatattatat tcaccgaagc ggtagaacgg ctagatttaa agatactggt
      121 aaagtaattt ctttacttga ttttgatgat gaaaagtatt tagctagatt acatggtcgt
      181 ggtttagata tttattttgg ggaaattata gagggaatgt ttaaggaaac taaatatcaa
      241 gaaagaaaga aaaacaaata tgatgaagaa attgaagttc ttcatgtaaa gtatccactt
      301 ccgccaaagg taaaaccaaa ttatcgtaag cgtagaaaag aaaaaattgc taaagaaaca
      361 agacaaatca aaagaaaaag agtagaagat atttataaga ggaaggctaa aaatgattaa
      421 gataggatct catgtaagta ataaaggcga tgagatgtta gtaggatcag ttttagaagc
      481 tgtaagctat gatgctaatt gttttatggt ttatttaggt gctcctcaaa actcatatcg
      541 taaagatttt gaaagtttta gaaaagatga atttcaacac ttattaaaag agcataaaat
      601 aaatattgat gatttaatcg ttcatgcgcc ttatattatt aatatggcta atcctgattt
      661 agaaaaaaga gagtttgcta ttgattttct aacaagagaa cttttattaa tggaaaactt
      721 aggatttaca aaaatggtag ttcatcctgg aaattcctta ggtgatgata atgcaataaa
      781 ttatttaaaa gaatcattaa ttaagatact agataacact aaaaatacaa atacgatgat
      841 tttactggaa acaatggcgg gaaaaggaac agaaatcggg agaaatttcg aggaacttgc
      901 ttttctttta gaaggattgc cagaagaaag agttaatgtt tgttttgata cttgccatac
      961 ttttgattca ggttatgata cttttaatga ttatgagggt gtattagaaa aatttgatga
     1021 ggtaattggt ttagaacgta ttaaagctat tcacttaaat gattcaaaaa atcctcttgg
     1081 atcacaaaaa gaccgacatg aaaatatcgg ctatggttac attggttttg aaacactatt
     1141 aaaatttgtt tatgatccaa ggtttaaaga tataccaaag gttttagaaa caccttatat
     1201 cgatggtaaa gcgccttata aggaagaaat agaaatgatt aaaatgaaaa agtttaatcc
     1261 taaattaaaa ggagagtctt aaaactctcc ttttatcatt ttatttattt cattttttaa
     1321 ttcttttaca atgttttcgg ctttgatttt tctaattgct tgaccttttt tgaataaaag
     1381 tgcttcacct tttccaccag caataccaat atcagcatct ttagcttcag ctggtccatt
     1441 aacagcacag cccataattg caacagttag ttttacgttg cctaaagtgt taagaaaaga
     1501 ttcgatttct ttagtgattt ccatcatatt atattcagtt cttccgcaag taggacagct
     1561 aattagaact ggtttattat ataagcctaa tgaagctaaa atatctttag caacactaat
     1621 ttcttcaact gggtcagctg ttagagaaac tctaatagtg tcgccaattc cctcataaag
     1681 taagattcca aggccaatac ttgatttaat cgttcccatt tgttttgggc cagattcagt
     1741 aattccaata tgtaatggat aactatagtt agcatttaag actttgtatg cttcaatagt
     1801 agcttctagg ttagtagatt taactgatag aataatgtta tgaaaatgaa atgattctag
     1861 aattctaata tttctaattg cactttcaac taaagcttct ttagaattac cgaatttttc
     1921 tgctagatct ttttcgattg aaccagaatt aatacctatt cttattggaa tattgtgttt
     1981 tttacacttt tctacaatct caccgatttc ttctttagtt ccaatattac ctggattaat
     2041 tctaatctta tcagcaccat tttcaattgc aagtaaagct aacttataat caaactgaat
     2101 gtcagctgct aggggaatat gtatttgttt ttttatttct tttaaagcaa gggcatcttc
     2161 cttagaagta atagcacatc taataagttc acagcctgct ttttctaatg ctagaatttg
     2221 actaacagta gcattgatat cactagtttt agtattagtc atgctttgaa ttaaaatagg
     2281 actattacca ccaatttctt tattaccaat aaagattttt ttagtttttc tcattgttag
     2341 ttttcctttt tttcttatgc ctttttggag atctgattct attttctctt aggatattgt
     2401 agattgtaga aggggccata tcatagccaa attcatcaat tagcatctct gcaaaatgac
     2461 taattagcat tcctctattt tcttttttac gataaagatc aacaatctct tttcttacct
     2521 cttcgctata agcaatagga ggacgatgga atctattttt atggattaca ccataatctc
     2581 cataagcata aactgcactt tttaagtttc taacttgacg tgtagatatt tttaaatatc
     2641 gtgctgccct tggtccatta tattctccca taatcgttct tctaatggca cgtaattttt
     2701 tagtttcttc tttagttaat tttctcggct ttatctctac aacagtacaa tcaccaatag
     2761 tagaagtaaa aacattgtat ttactcattt tatcaccttt cataaaaaaa gatattgttt
     2821 caatatcttt atttctttgt tttctcttta tgtaatgttt gttgtctaca tctagggcag
     2881 tattttttca cttcaagacg atcaggagtg tttttcttat ttttttctga tagataattt
     2941 tcctctccac aaactgtaca tcttaagata aatgttactc tcataatatc ctcctgttta
     3001 tctttgatat tatactataa aagtgcctga aaagcaagaa ttatattaat tatttttggc
     3061 tttttcaatt gcctctaatc tttcttttaa ggctttttca tattttcctg tttcttttgg
     3121 atcataatat tttgcatcct taattatatc aggtaggtat tgttgcttta cccaagcgcc
     3181 tggataatca tgaggatata aataactctt ttggcttgga tcaaaagaat aagtgttttt
     3241 caaattaagt ggaattggac ctgttttacc tttttcaaca tcctcaatcg ctttactaac
     3301 tgctaaataa gctgagttag attttggtga taaagcaagg tcaattacaa taacagatag
     3361 tggcagttta gcctcaggca tccccaaatc taaagctatt tcgcatgctg catgaacttt
     3421 tggcccaagt cctgggtttg ctaagcctat atcttcataa caaattgcat aaagccttct
     3481 aattaaagga agtaaatcat cagccattaa gagtcttgca agataatgaa gagctgcatt
     3541 aacatcagaa cctctaattg atttttgcag gccacttaaa gtgtcatagt agttttcacc
     3601 atctttatca attgaaacag acgcaatttg aattgttctt tggacatcat ctaagcttac
     3661 ttgctctgta tcaattgtat ttacagttgc ttctaagtag ttaagtaatg atcttacatc
     3721 gccattagcg caagaaataa gatatttctt ggcgtcattt gaaaacttat gatgtgattc
     3781 taagtgttct aagccatcgt agaagatctt ttctaaatct tgatattgta atggcttaag
     3841 acgataaatt aaagtcctag atcttaccgg tggatttaaa gaatgataag gattgattgt
     3901 agttaaaccg atgattgtaa tagaaccatc ttctagatga ggtaaaagat agtcttgaat
     3961 atcttttttc attctgtgaa tctcatcaat tattaaaata gaatctgaat gattatcaat
     4021 aatctctttt agggtagctt tgttatcgtt agaagcatta aataattgat atggtctatt
     4081 taattcatta gcgattacaa ttgctaaggt agtcttacca attccaggct caccatataa
     4141 aacaagtgat tgaagtttat tgtttttaac catcctaaaa ataggaccat ttgttcctat
     4201 caaatgatct tgaccataga tattactaaa cttttttggt ctaattttcg atgctaatgg
     4261 ttccataatc cacc
//
LOCUS       DAOSGS010000075         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_18693_length_4269_cov_4.318700, whole genome shotgun sequence.
ACCESSION   DAOSGS010000075 DAOSGS010000000
VERSION     DAOSGS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18693_length_4269_cov_4.318700"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1563)
                     /locus_tag="PLQ22_03540"
     CDS             complement(46..1563)
                     /locus_tag="PLQ22_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH59066.1"
                     /translation="MFELKNLSKYFGSVIANEDISLKINEGEILALLGENGSGKTTLM
                     NMLAGLYFPDSGQIFHEGEEIVIKSPKDASDHGIGMIHQHFKLVDLLTAAENVILGLN
                     KGFLLDREKIEQEIKEVADMYGFNIDPKKKVYNMTVSEKQTLEIVKVLYRGANLLILD
                     EPTAVLTPQEAKQLFKALQRLKERGKAIVLITHKMNEVLEVSDNIAVLRKGRLVARLD
                     TKDATELKLAELMVGEKVSLNIKRSKVKDPVLRLEIRNLSVKNLYGRTVLDDVSFDAY
                     GGEILGIAGISGSGQKELLEAIAGLEHAQKGSDITYYHPKHNEVKLIGKSPLQIKRLG
                     ISLSFVPEDRLGMGLVGSMGMVDNVLLRTYDETPNFYKNRKRAKKIAKEIKDELAVST
                     PSEDTAVQTLSGGNVQKVLVGREIANNPIVFMTAYAVRGLDINTSYVIYDILNKQKEA
                     GVAVIYVGEDLDVLLALSDKILVLSNGSISKPLDPKKVTKEEIGLLMTNKMVKES"
     gene            complement(1634..2869)
                     /locus_tag="PLQ22_03545"
     CDS             complement(1634..2869)
                     /locus_tag="PLQ22_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH59067.1"
                     /translation="MKKFVLLLLAVFSLVGLVGCSCKEEKGDGEFKVGFILIGDENEG
                     YSLAHLNGINQMKKALGLKDSQIVFKKHTPEGKEAQDAAEELVGAGCKMVFANSFGHE
                     EGLFTVAAEHPEVHFLHATGIRAKSSGLPNVSNFFVDIFEARYVAGIYGGVRLNELIA
                     QNADKYAPGNKVTIGYVGAKAFAEVKSGFTAFYLGVRSALDPAYELEMKVQYTGEWAN
                     QVAEKNAATALIDSGCVLISQHADTVGAATACEEKSINNVGYNVSMLDAAPTQAITSA
                     GLNWGVYYTYAVREGMAGRKPAVDWCEGFNKGAVAITEVNRNAFGSDAAYNEANQKAE
                     AAIAAIKAGTLHVFDTSKFTVGGEKVTSTKDVAGFYGKEYIKTKDGVSYFAESDDALG
                     SAPGFAFVIDGIIELNQAF"
     gene            complement(2884..3453)
                     /locus_tag="PLQ22_03550"
     CDS             complement(2884..3453)
                     /locus_tag="PLQ22_03550"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017400424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOH59068.1"
                     /translation="MEALKKKILEEGRVFPGEILKVDSFINHQIDVDLLDKIGKHIAS
                     KFPDVTKIVTIETSGIAFAVGVSYHLGKVPVVFAKKEESAITKDDNYYAPLYSYTKKK
                     EYQAFISKKHLSENDRVLIVDDFLAKGNAVIALMEIIKRSNAEIVGVGIVIEKAFQSG
                     RERILSQGLKLESCVKIASLSDGIKFLSK"
     regulatory      complement(3530..3630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(3755..3847)
                     /locus_tag="PLQ22_03555"
     tRNA            complement(3755..3847)
                     /locus_tag="PLQ22_03555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3811..3813),aa:Ser,seq:gct)
     gene            complement(3928..>4269)
                     /locus_tag="PLQ22_03560"
     CDS             complement(3928..>4269)
                     /locus_tag="PLQ22_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59069.1"
                     /translation="TRTYVFLYDSTDTSDYCKEVEDFVIAYATRAVRENRIKVYTIDV
                     SKEENKFLVNDHEEVPEKINTVTDYEYMRIRSKKIPILLIVDTKKVSYYKTGLNSIKQ
                     EMQTELEKVQK"
BASE COUNT         1384 a          858 c          506 g         1521 t
ORIGIN      
        1 tatctaatct tagataacta tctatagcac tacttaaaaa catgtttaag actcctttac
       61 cattttgtta gtcattaata aaccaatttc ttctttagta acctttttcg gatcaagtgg
      121 tttactaata ctaccattac tcaaaactaa aatcttatca gataaagcaa gcaaaacatc
      181 taaatcttcc ccaacataaa taacagctac ccctgcttct ttttgtttgt ttaaaatatc
      241 ataaattaca taagaagtat taatatctaa acctctaaca gcataagcag tcataaacac
      301 aattgggtta tttgcaattt ctcttccaac taatactttc tgaacattac caccagataa
      361 tgtttgaaca gcagtatctt cagaaggagt cgaaacagca agttcatctt taatttcttt
      421 agctattttc tttgctcttt ttctattttt atagaaatta ggtgtttcat cataagttct
      481 taaaagaaca ttatcaacca tacccatact acctacaagt cccataccta atctatcttc
      541 aggtacaaac gataaagata ttcctaatct tttaatttgt aatgggctct taccaattaa
      601 tttaacttca ttatgctttg gatgatagta tgtaatatca ctaccttttt gggcatgctc
      661 taaacctgca attgcttcta aaagttcttt ttgaccagat ccagaaatac cagcaattcc
      721 taatatctct ccgccataag catcaaaaga aacatcatct aaaactgttc ttccatataa
      781 attttttact gataaatttc taatttctaa tcttaaaaca ggatccttaa ctttagatct
      841 tttaatatta agacttacct tttccccaac cattaactct gcaagtttta attcagttgc
      901 atctttagta tcaagtctag ctacaagtct accttttctt aaaacagcta tgttatctga
      961 cacttctaat acttcattca ttttatgagt tattaaaaca atagccttgc ctctttcttt
     1021 taatctttgt aaagctttga aaagttgctt agcttcttgt ggagttaaaa cagcagttgg
     1081 ttcatccaaa atcaaaagat tagctccacg atataagacc tttacaatct ctaaggtttg
     1141 cttttcagaa acagtcatat tataaacttt cttttttgga tcaatattaa atccatacat
     1201 atcagctact tctttaatct cttgctctat cttttctcta tctaaaagaa agcctttatt
     1261 tagacctaaa ataacattct ctgcagctgt taataaatca actaatttaa agtgttgatg
     1321 aatcattcca atcccatgat cgctagcatc tttaggactt ttaataacaa tttcttcacc
     1381 ttcatgaaaa atctgcccac tatcaggaaa atataaccca gcaagcatat tcattaaagt
     1441 cgtcttaccc gaaccatttt ccccaagtaa agctaaaatt tcaccttcgt tgattttaag
     1501 ggaaatgtct tcattagcaa ttacacttcc aaaatatttt gataagtttt ttagttcaaa
     1561 catttatctc tccttatata taaaaatgaa gaaggcaaaa taattgcctt cttctcataa
     1621 ccttaatcta tttttagaaa gcttgattta attcaataat tccatcaatt acaaaagcaa
     1681 atcctggagc tgaacctaga gcatcatctg attctgcaaa atagcttact ccatcttttg
     1741 tcttaatata ttctttacca tagaagcctg ctacgtcttt agttgaagta actttttctc
     1801 cacctactgt aaatttagat gtatcaaata catgtaaagt accagctttg attgctgcaa
     1861 tagcagcttc agctttttga ttggcttcat tatatgctgc atcgctacca aaagcatttc
     1921 tatttacttc agtaattgca actgcacctt tattgaatcc ttcacaccag tcaactgctg
     1981 gttttctacc tgccatacct tctctaacag cataagtata atatactccc cagtttaatc
     2041 cagctgatgt aattgcttga gttggagcag catctagcat tgaaacgtta taaccaacgt
     2101 tattaatact tttttcttcg caagctgttg cagcacctac tgtatcggca tgttggctaa
     2161 ttaaaacaca acctgaatcg attaaagctg ttgcagcgtt tttctcagct acttggttag
     2221 cccattcacc agtgtattga actttcattt ctagttcgta agcaggatca agtgcacttc
     2281 taactcctaa atagaaagca gtaaaaccag atttaacttc ggcgaatgct ttagcaccta
     2341 cataaccaat agtaacctta tttcctggtg catatttatc tgcattttga gcaattagtt
     2401 catttaatct aactccacca taaatacctg ctacatatct tgcttcaaaa atatctacaa
     2461 agaaattaga tacgtttgga agaccacttg actttgctct aataccagtt gcatgtaaga
     2521 aatgaacttc aggatgttct gcagctactg taaataaacc ttcttcatga ccaaagctgt
     2581 tcgcaaaaac cattttacaa ccagcaccta ctaattcttc tgcagcgtct tgtgcttctt
     2641 taccttctgg agtatgtttc ttaaaaacaa tttgtgaatc ttttaagcct aatgcttttt
     2701 tcatttggtt aataccgtta agatgcgcta aactatagcc ttcgttttca tctccgatta
     2761 aaataaatcc aactttaaat tctccatcac ctttttcttc tttgcaactg caaccaacta
     2821 aacctactaa actgaatact gctaataata gtaaaacaaa ctttttcatt ttccctcctc
     2881 gttttattta ctcaaaaact taataccgtc actaagactt gcaatcttaa cacaagattc
     2941 taattttaaa ccttgactta agatcctttc tcttccgctt tggaatgcct tttcgataac
     3001 tatacccact cctacaatct cagcattaga tcgtttaatt atttccatta aagctataac
     3061 tgcattacct ttagctaaaa aatcatcaac gattaaaacg cgatcatttt cgctcaaatg
     3121 ctttttagag ataaaagctt gatattcctt tttctttgta tatgaataca aaggggcgta
     3181 ataattatca tctttagtaa ttgcagattc ttctttcttc gcaaacacaa ctggaacttt
     3241 acctaaatgg taagatacac caaccgcaaa agcaattcca ctagtttcaa tagtaactat
     3301 tttagttaca tcagggaatt tacttgcaat gtgtttacca atcttgtcaa gcaaatcaac
     3361 atcaatttga tgattaataa aagaatcaac ttttaaaatc tcgcccggaa aaactcgtcc
     3421 ttcttctaaa attttctttt ttaatgcttc cataaagcct cctgaaaata aaaaaacttt
     3481 cttaaacaat tagtttgaga aagtaaaatg aaaaaggatg tccacaaaaa agtaaacatt
     3541 cttaattcgt agtgccagca tttacggtgt ggcgtagaaa ctttccctcc atattaggat
     3601 aattatacga aaactataat gtcttcaaaa gacattatta ttatatacca ttattatgca
     3661 tttgtctaat tgaaaacgct ttatattttc aagtaaatat cacttgaaaa taaaaaacaa
     3721 attagattat aaacctaatt tgtgtgttat caaatggcgg agtaggagag atttgaactc
     3781 tcgcatcggt gattaccgat ctacaccctt agcaggggcg cctcttcagc ctcttgagta
     3841 ctactccatg ccaatgtatt agtattttac tttatttttg agtttttgtc aagtataagc
     3901 taattaaaaa gaagtgatta tcctaaatca cttctgaact ttttctaatt cagtttgcat
     3961 ttcttgctta attgaattta aaccggtttt ataataacta acttttttag tatctacaat
     4021 caataaaatt ggtatttttt tagacctgat tctcatgtat tcataatcgg taacagtatt
     4081 aattttttct ggaacctctt cgtgatcatt aactaagaat ttattttctt ctttactaac
     4141 atctatagta taaaccttaa ttctattctc acgaacagct cttgtagcat aagctataac
     4201 aaagtcttca acttctttac aataatcact tgtatcagtg ctatcataga gaaaaacata
     4261 agtcctagt
//
LOCUS       DAOSGS010000076         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_18714_length_4265_cov_6.097625, whole genome shotgun sequence.
ACCESSION   DAOSGS010000076 DAOSGS010000000
VERSION     DAOSGS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18714_length_4265_cov_6.097625"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLQ22_03565"
     CDS             complement(<1..588)
                     /locus_tag="PLQ22_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOH59070.1"
                     /translation="MIKIKINQEEYFVTITKKRIKNINLKIDEQNRITISCPLHMQEQ
                     EVVTFLDRHREWLTKRIMKNNKNKQQFIHLQDEKIVYLLGKEYLIVATKSLDKHLQIE
                     DDVIYFASKKSFYSFYHDYLINRAKELCEEKGIEATIKVKRYRSRWGCCKYRTKEIYL
                     NEKLIALPKDFIDYVIYHEISHLIVPNHSPSFYKTL"
     gene            complement(604..1302)
                     /gene="radC"
                     /locus_tag="PLQ22_03570"
     CDS             complement(604..1302)
                     /gene="radC"
                     /locus_tag="PLQ22_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOH59071.1"
                     /translation="MKYTLKEMPIEERPRERLVKYGAKALSNQELLAILLTTGTKGVS
                     VVEVASRLLITLKGIQNFNEVTIKELTKITGIGTAKAIMLLAAIELGKRINSNQSKHQ
                     VISSPQDAYHYLQANMSTLSQEHLVCLYLNVKSEVIEQRTISIGTVNTTVFNPKDIFK
                     WALKLSASFVIVAHNHPSGDPTPSSNDTLMTKKIVATGKAVDIEVVDHIIIGKGRYYS
                     FLEHRQGKRLIADD"
     gene            complement(1868..3007)
                     /locus_tag="PLQ22_03575"
     CDS             complement(1868..3007)
                     /locus_tag="PLQ22_03575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH59072.1"
                     /translation="MKGRLDMRILMINSVCGIKSTGRICIDLAKKFLIEGHEVRIAYG
                     RGIVPSEYQSISIPICNKKDVYLNALKARLFDNEGFNCKTTTAKFLKFAEKYDPDLLW
                     LHNLHGYYINVEMLFRWIKSRPNMQIRWSIYDCWAFTGHCSHFSYVGCNQWQTYCKKC
                     IQKKQYPKSYIDNCKENFERKKIAFCGIKNMTLISPSFWLEKLIKKSFLKEYPIKVVH
                     ITTDDKNFKPTESNFRTENHLLDKKIVLGVASVWNERKGLYDFIKLRNILDKSYEIVL
                     VGLSKKQMVKIRKKIVCIPRTDNTKELAKIYTAADVFVNPSKEETFGLTNLEALLCGT
                     PVIVYKNTACEEVINLYGNGVAVDNSIEALATEIIMTVEKRKNST"
     gene            complement(3325..4119)
                     /locus_tag="PLQ22_03580"
     CDS             complement(3325..4119)
                     /locus_tag="PLQ22_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOH59073.1"
                     /translation="MLGAIVGDIVGSRYEFKNYKSKEFELFHTDCFVTDDTIMTLAVA
                     EAIMDCQGDYTKLYDATIASMRLIGPKYPCSGYGGSFFRWLYSDRPKPYDSYGNGAAM
                     RISPVAYIATSLADVKKYSRAVTETTHNNIEGIKGAEAVASAIFLARSGKTQAEIRDY
                     ICHNYYPLNFTLDEIRPTYEFDSSCQGTVPQAIVAFLEANSFEDAIRNAISIGGDADT
                     IAAITGSIAEAYFSIPEEIAEKALLYLDELQLDILQRFENFRKVLR"
BASE COUNT         1338 a          734 c          584 g         1609 t
ORIGIN      
        1 aagggttttg tagaaggaag ggctgtgatt ggggacaatc aagtgactga tttcatgata
       61 gatgacataa tcgatgaaat ccttcggtaa agcgatgagt ttttcattca agtaaatctc
      121 tttggtgcga tacttgcaac aaccccatct gcttctatat ctcttcactt ttatcgttgc
      181 ttcgattcct ttttcttcac acaattcttt tgctcggttg attaaatagt catggtagaa
      241 agaataaaaa ctttttttgg aggcaaagta gattacatca tcctcaatct gcaaatgttt
      301 atctagactt ttcgttgcca caattagata ttcttttcct aacaaataaa ctatcttttc
      361 atcttgtaaa tggatgaatt gttgtttgtt tttattgttt ttcataattc ttttggttag
      421 ccactcgcga tggcgatcaa gaaaagttac tacctcttgt tcttgcatat gtaaggggca
      481 actgatagta attctatttt gttcatcgat ttttaaattg atgtttttga ttcgtttttt
      541 ggttatcgta acgaagtatt cttcttgatt gattttgatt ttaatcattg tatcttcctc
      601 tatttaatca tcagcgatta atctttttcc ttggcggtgt tctaagaaac tatagtagcg
      661 acctttaccg ataatgatat ggtcaactac ttcaatatca acagcttttc ctgttgcaac
      721 aatctttttt gtcattaagg tgtcattact actcggagta gggtctccac ttggatgatt
      781 atgagcgact atcacaaaac tagcggataa ttttaaagcc catttaaaaa tatctttggg
      841 attaaaaaca gtggtattga ctgtaccaat ggaaatcgtt cgctgttcta ttacttcgct
      901 tttcacgttt aaatataaac aaactaaatg ttcttgagac aatgtgctca tattcgcctg
      961 taaataatga taggcatctt gtggcgaaga tatgacttgg tgtttacttt gattagagtt
     1021 tatccgtttt cctagttcga ttgcagcgag caacataatc gctttcgccg ttcctattcc
     1081 ggtaatcttc gttaattctt taatcgtcac ttcattaaag ttttgtatac ctttaagagt
     1141 aatgagtaat ctacttgcga cttcaacaac cgatacacct ttagttccgg ttgttagtaa
     1201 aatcgctagt aattcctgat tagatagagc tttagctcca tatttaacta gccgttctcg
     1261 ggggcgttct tctataggca tttctttcaa agtgtatttc atagtttccc tctttaccaa
     1321 aatataaata actatattta tatattatgg aagagttcat ttttttactt taaaaaagac
     1381 ttattatcta agtccttttc gaaatttttc aaatctttgt aagatatcga gttgcaattc
     1441 atctaaatag caagagtttt ctaaaaaatc aaaatatttt ttcattaaaa ctagtactag
     1501 attattcgta gatttgattc ctttcgaggt ataattgagg atagctaatt taaatataat
     1561 tagaaattta acttcaattt tatttttcta ctttcaataa tattttgctg tttttttgct
     1621 cagatattct ttaacttcat caattatccc tatacgaaat gttcgagggt tatactcaaa
     1681 tgctctttga acatcatcgt tgggaaaaac tctcggttca cataaacgtt gtactttttc
     1741 tcgataatct ttatatccaa acgatataat atataaatac cacgctccaa tatacgcaag
     1801 agaaaacgga caattaataa atcttacttt tttctcaagg ttttccctaa ttatgctaag
     1861 catatcccta agttgaattt tttctctttt ctactgtcat tataatctcg gtagccaaag
     1921 cttcaataga gttatcaaca gcaactccat taccataaag attgattact tcttcgcatg
     1981 ccgtattctt atatacaata acaggagttc cgcataacaa agcttcgaga tttgttaacc
     2041 caaatgtttc ttctttgctt ggattgacaa atacatcagc tgctgtataa atcttcgcaa
     2101 gttcctttgt attatccgtt ctaggaatac atactatttt ttttcgaatt ttcaccattt
     2161 gtttttttga taacccaact aaaactatct cataggattt atctaatata tttcttagtt
     2221 ttataaaatc ataaagtcct ttgcgctcat tccacacact agccacaccc agcactattt
     2281 tcttatccaa caaatgattt tcagttctaa aattactttc tgtaggtttg aaatttttat
     2341 catctgtagt aatatgtaca actttgatag gatattcttt taaaaatgat ttttttatta
     2401 atttttcaag ccagaatgat ggagagatta gagtcatatt cttaattcca cagaaagcta
     2461 ttttctttct ctcaaaattt tctttacagt tatcaatata tgattttgga tattgctttt
     2521 tttgaataca ttttttgcaa taagtttgcc attgattgca accaacatac gaaaaatgtg
     2581 aacaatgacc agtaaatgcc cagcaatcat atatgctcca tctaatttgc atattaggac
     2641 gagacttgat ccatcgaaaa agcatttcaa cattgatata atatccatga agattatgca
     2701 accatagtaa atcaggatca tatttctcag caaattttaa aaatttagct gtcgtagttt
     2761 tgcaattaaa cccttcatta tcaaacaagc gagcttttaa agcatttaaa tatacatctt
     2821 ttttattgca tattggtata gatatagatt gatactcaga aggaactatg ccacgaccat
     2881 aagcaattct tacttcatgt ccttctataa gaaatttttt ggccaaatct atgcaaattc
     2941 tacctgtact tttaattcca catactgagt taatcattaa tattcgcata tctaatctac
     3001 ctttcataat atatctaatg ttttatattg aattttttgc cttttttatt atacaagtaa
     3061 ttttcacata aatagtctaa atattttact tgtaattatt tatgaactat gtacattttt
     3121 ctcaatcata actttaacaa aatattcttt ataaaaaatc gtatttttaa atgcacttat
     3181 ttaatttttt atggctcaat cattttgtga tatttcaatt gcaaaatggt gatgttacat
     3241 tgtcaataca attagttctt ttccaaattt catttattaa ttcatttgat gaaagtatgg
     3301 tcaaatattt taaaaaagac ttaattatct aagtactttt cgaaaatttt caaatctttg
     3361 taagatatcg agttgcaatt catctaagta aaggagcgct ttttcagcta tttcttcagg
     3421 aatagaaaaa tacgcttcag caatcgaacc agtaatcgca gcaattgtat ctgcatctcc
     3481 tccaatactg atggcatttc gaatggcatc ttcaaagcta ttggcttcta agaaagcaac
     3541 aatcgcttga ggaacagttc cttggcaact tgaatcaaat tcataagttg gtcttatttc
     3601 atctaaggta aagttaagcg gataatagtt gtgacaaata taatctctaa tctcggcttg
     3661 cgttttaccc gaacgagcta aaaagattgc actagcaact gcttctgctc ctttaattcc
     3721 ttcaatatta ttatgcgttg tttcagtaac agctctgcta tattttttaa catcagctag
     3781 tgaagtagca atataagcaa ctggactgat tcgcattgct gcaccattac cataactatc
     3841 gtatggtttt ggtcgatcag aataaagcca acgaaagaaa ctacctccat aaccagaaca
     3901 aggatatttt ggtccaatta atctcatact agcgatagta gcatcgtaaa gctttgtata
     3961 atcaccttga cagtccataa ttgcttcagc aacagctaat gtcataatag tatcatccgt
     4021 aacaaaacaa tcagtatgaa acaattcaaa ttctttgctt ttatagtttt tgaattcata
     4081 acgtgaacct acaatatcac caacgattgc tcctagcata gtagatttat cctcctatat
     4141 catatttaca cctttattat aaaataaaat tgtaaataat acaatacaga taaaatagtt
     4201 tatattcata aatggattaa ctgactttat taatattttg ataatcaaat cttttaattt
     4261 aaaat
//
LOCUS       DAOSGS010000077         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_18866_length_4241_cov_3.586001, whole genome shotgun sequence.
ACCESSION   DAOSGS010000077 DAOSGS010000000
VERSION     DAOSGS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18866_length_4241_cov_3.586001"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLQ22_03585"
     CDS             complement(<1..383)
                     /locus_tag="PLQ22_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59074.1"
                     /translation="MKRNKEVIDITLSGQPKMKKAQANLGDFYHEMKEIKKDKDPYLE
                     YSLDQKNEKITKFINEFDNTSEPFFKRLYTIILFQEGIKKDLKFKINIEQVYNSSIIL
                     KEASKTLKVEYESVDKANVEEVDVI"
     gene            complement(487..1800)
                     /gene="eno"
                     /locus_tag="PLQ22_03590"
     CDS             complement(487..1800)
                     /gene="eno"
                     /locus_tag="PLQ22_03590"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH59075.1"
                     /translation="MPFINEIYAREVLDSRGNPTIEVEVYTESGGFGSAIVPSGASTG
                     AYEAVELRDGDKKRYLGKGVLKAVANVNDLIAPELIGFNVFDQVGIDMAMCELDGTPN
                     KGKLGANAILGVSIACARAAADLCGLSFYNYIGGFNARTLPTPMLNILNGGSHADNNV
                     DFQEFMIMPVSAKSFAEGIRVATEVFHTLKKVLKKDGKNTAVGDEGGFAPNLGSNEEA
                     LQVIMQAIKEAGYVPGKDIMLAMDVAASEFYNKETKKYVLAGEGGKELSSKELADFYV
                     ELVEKYPIISIEDGLDEDDWDGWKYLTEKLGKKIQLVGDDLFVTNTARLQKGIDLGVA
                     NSVLIKVNQIGTLTETLETVELAKKHNYTAVISHRSGESEDTTIADLAVALNTGQIKT
                     GSASRTDRIAKYNQLLRIEDELGDTSKYLGGEAFYNIKKAKKAKK"
     gene            complement(1800..3326)
                     /gene="gpmI"
                     /locus_tag="PLQ22_03595"
     CDS             complement(1800..3326)
                     /gene="gpmI"
                     /locus_tag="PLQ22_03595"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH59076.1"
                     /translation="MKRLVMLTILDGFGLSDNKDNNAIYAAKKPNLDYLFKEYPNNTL
                     NASGEAVGLPEGQMGNSEVGHLNLGAGRIVYQSLTRVNIAVRENTLIKNEVIQKAIKH
                     AKKNKSKVHIMGLLSDGGVHSHIKHILYLVEKIVEAGVKEVVVHAYLDGRDTPPKSAL
                     TYLKELQETIDKVGNSKIGIVSGRYYAMDRDRNWDRVQPAYEALVYNKGAVKNVFKGI
                     EESYANGVTDEFVYPYIANLDSNINDGDSVIFANFRKDRAIEMSMALTNLSMTGIQNG
                     KVFKDLFYASMMEYSENVKGEVILPDLVLENIYGEIIEKAGMTQLRIAETEKYAHVTF
                     FFDGGEERTFKGADRILVPSPKEVPTYDLKPEMSAFEVCAKAEEAIASKKYDTIILNF
                     ANCDMVGHTTVFDATVKAVETVDTCVGRLYEAVKKVGGVMIVIADHGNAEKLVEDGKP
                     FSAHTLNPVPFIITDKSYKVRSGGNLGDVAPTLLELLGIKKPVEMTGKTLIYKNKEEF
                     "
     gene            complement(3413..4030)
                     /locus_tag="PLQ22_03600"
     CDS             complement(3413..4030)
                     /locus_tag="PLQ22_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOH59077.1"
                     /translation="MKELEMMSSPFSKGYWRNAIKEFSSLKNVVLIGLLIALQVVVGL
                     LYIPVTAEVRIKFDFIVSSLIGMIFGPLVGMVSGAISDVLSFVIHPTGPFFIGYTLTS
                     ALAGFIYGIFFYRTKIKFWKLIAAKLIVNMVCNAFLGTLWYYIYMSKDLVFEALITSF
                     WIIFTKNIVLLPLEVMVIYTIFKQLFLVLETKKLIVLQDPAMKWF"
     regulatory      complement(4060..4151)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
BASE COUNT         1368 a          829 c          487 g         1557 t
ORIGIN      
        1 ataatcacat ccacttcttc aacatttgct ttatcaacac tttcatattc tacttttaaa
       61 gttttactag cttcttttaa aataatagag ctattataaa cttgctcaat attgatttta
      121 aatttcaaat cttttttaat accttcttgg aataagatga ttgtatataa acgtttgaag
      181 aatggttcag aagtattatc aaattcattg ataaacttag taattttttc atttttttga
      241 tctaaagaat attctaaata agggtcttta tcctttttaa tttctttcat ttcatggtaa
      301 aaatcaccca agtttgcttg agctttcttc attttaggtt gtccagataa tgtaatatca
      361 attacttctt tattcctctt catttaaatc acctatataa tattataaca taatttaatc
      421 tattttcata gttaaaaaca aaagtcccta tttaagaata aggacttttt tatttaaaat
      481 aaattattat ttcttagctt ttttagcttt tttgatatta tagaaagctt ctccacctaa
      541 gtatttagat gtatcaccta attcatcttc aattcttaat aattgattgt acttagcaat
      601 acgatctgtt ctactagcag aaccagtttt aatttgacca gtatttaatg ctacagctaa
      661 atctgcaata gttgtatctt cagattcacc tgatctatgt gagataactg ctgtatagtt
      721 atgtttctta gcaagttcta cagtttctaa tgtttcagtt aatgtaccaa tttggttaac
      781 tttaattaaa actgagtttg caactcctaa gtcaatacct ttttgtaatc ttgcagtgtt
      841 agttacaaat aagtcatccc ctacaagttg aattttcttt cctagttttt ctgttagata
      901 tttccaacca tcccaatcat cttcatctaa tccatcttcg attgaaataa taggatattt
      961 ttctactaat tctacataaa aatcagctaa ttctttagat gataattctt ttccaccttc
     1021 accagctaaa acatatttct tagtttcttt attatagaat tctgatgcag caacatccat
     1081 tgctaacatg atatctttac caggaacata tcctgcttct ttaatagctt gcataataac
     1141 ttgtaaagct tcttcgttac ttcctaaatt aggtgcaaat ccaccttcat cacctacagc
     1201 tgtgttcttt ccatctttct ttaaaacctt tttaagagta tggaatactt ctgtagctac
     1261 tctaattcct tcagcgaaac tcttagcact tacaggcata atcataaatt cttggaaatc
     1321 tacgttgtta tctgcgtggc ttccaccatt taagatatta agcattggag ttggaagtgt
     1381 acgagcattg aatccgccaa tgtagttata gaaagataaa ccacataagt cagcagctgc
     1441 tcttgcacaa gcaatagaaa ctcctaaaat agcattagca ccaagtttac ctttgtttgg
     1501 tgtaccatca agttcacaca tagccatatc gattcctact tgatcaaata cgttgaatcc
     1561 aattaattca ggtgcgatta aatcattaac attagctact gcttttaata ctcctttacc
     1621 taaataacgt tttttgtctc catcacgcaa ctctacagct tcataagctc ctgttgatgc
     1681 tccagatgga acgattgctg aaccaaagcc cccactttca gtgtaaactt caacttcgat
     1741 tgttggatta cctcttgaat ccaatacttc tcttgcataa atttcattaa taaatggcat
     1801 taaaattcct ccttattttt atatattaat gtctttccag tcatttcaac tggtttttta
     1861 attcctaaca attctagtaa tgtaggtgca acatcaccta aattaccacc acttcttact
     1921 ttataacttt tatctgtaat gataaatgga actgggttta atgtatgagc ggaaaatggt
     1981 tttccatctt ctacaagctt ctctgcatta ccatgatcag cgataactat catcactccg
     2041 ccaactttct taacagcttc atacaatctt ccaacacaag tatcaactgt ttctactgct
     2101 ttaactgtag catcaaaaac tgttgtatga cctaccatat cacagttagc aaagtttaag
     2161 ataattgtat cgtatttttt agatgcaatt gcttcttctg ccttggcaca tacctcaaag
     2221 gcactcattt ctggtttcaa atcatatgtt ggtacttctt ttggagaagg tactaaaata
     2281 cgatctgccc ctttaaatgt tctttcttct ccaccatcaa agaagaatgt aacgtgagca
     2341 tatttttccg tctccgcaat cctaagttgc gtcatccctg ctttttcaat aatctcacca
     2401 tagatatttt ctaaaacaag gtcaggtaaa attacttctc ccttgacatt ttctgaatat
     2461 tccatcattg aagcatagaa taaatcttta aatactttcc cgttttgaat tcctgtcata
     2521 cttaagttag ttagcgccat cgacatctca attgccctat cttttctaaa gttggcaaag
     2581 attactgaat caccatcatt gatattagaa tctaaattag caatgtatgg ataaacaaac
     2641 tcatctgtta ctccatttgc atatgactct tcaattcctt taaaaacatt cttaacagca
     2701 cctttgttat aaactaaggc ttcatatgca ggttgaacac gatcccagtt acgatcacga
     2761 tccattgcat aatatctacc actcacaata ccaattttag aattaccaac tttatctatt
     2821 gtttcttgta attcttttaa gtaagtaagt gctgatttag gtggtgtatc tctcccatct
     2881 agataagcat gaacaacaac ttcttttact cctgcctcta caatcttctc aactaaatac
     2941 aaaatatgtt tgatatgtga gtgaactcca ccatctgata atagccccat tatatgaact
     3001 ttacttttat ttttcttagc atgttttatt gctttttgaa ttacttcatt tttaattaaa
     3061 gtattttctc taacagcgat attaactctt gttagagatt gataaacaat tctaccagca
     3121 cctaaattta aatgtcctac ttcactatta cccatttgtc cttcaggtaa acctacagct
     3181 tcacctgatg catttaatgt gttattagga tattctttaa ataaataatc taaattaggt
     3241 tttttagctg cgtaaattgc attattatct ttattatcac ttagaccaaa tccgtctaag
     3301 attgtaagca taactaatct cttcatattc tctcctcaaa ttccttatct attatatcaa
     3361 attcaaacaa tcttttcaat taaggtaaaa aagaaaatgt tttatattcc aattaaaacc
     3421 atttcattgc tggatcttgg agcacaatta acttcttggt ctccaaaact aaaaataact
     3481 gcttaaaaat tgtataaatt accattactt ctaaaggtaa aaggacaata ttctttgtaa
     3541 atataatcca aaatgaagta attaaagctt caaatactaa atccttactc atataaatat
     3601 aataccaaag cgtgcctaaa aaagcattac ataccatatt cacaattaac ttagctgcaa
     3661 tcaatttcca aaacttgatt ttagttcgat aaaagaaaat cccataaata aaaccagcca
     3721 gtgccgatgt taaggtatag ccgataaaga atggtcctgt aggatgaatc acaaaactta
     3781 agacatcact tatagcacca gagaccatcc ctactaaagg accaaaaatc attcctatta
     3841 aacttgaaac tataaaatca aattttattc ttacttctgc tgttacggga atatataata
     3901 aaccaactac aacttgtaaa gctattaata accctattag tactacattt tttaaactac
     3961 taaattcttt tattgcattg cgccaataac ctttactaaa agggcttgac atcatttcta
     4021 attctttcaa tttaagacct cctattttaa gagatccacc ataagtggat taaactagaa
     4081 aaccgccatt aaatgttcgt caatattcct ccacaaaaat attgcactta acgctttcta
     4141 gctactctat attttatcta aaaaaggtta taaaattata accttattta aatctattct
     4201 ttagccaatc aggaatgttt gaatcacctt tagatctttt a
//
LOCUS       DAOSGS010000078         4151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_19436_length_4151_cov_3.871582, whole genome shotgun sequence.
ACCESSION   DAOSGS010000078 DAOSGS010000000
VERSION     DAOSGS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19436_length_4151_cov_3.871582"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLQ22_03605"
     CDS             <1..313
                     /locus_tag="PLQ22_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59078.1"
                     /translation="KKMPSGESRAQIFHVSDKVAFEKLFKKYFSKYFILLSKEEIMKL
                     YGKGIPHKTLKTSLGDYVAVATNKYNFVAKDGEPMKGHHGGFTYKEMRIPLIIGVKNG
                     I"
     gene            303..776
                     /locus_tag="PLQ22_03610"
     CDS             303..776
                     /locus_tag="PLQ22_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59079.1"
                     /translation="MEYRTIGYKSYGFILFYGIIEAFLITAVVVFTQKYLETKENLAI
                     LIIASALVLVALVLFIFEFLEPKKKIQIDEKSLKLRFRNKETVILFDDFLDANIYGKS
                     VISKSGKLKILTKKGFHFVENVADVEVVKTALLQAAYSYKIKEIERQEGESKNED"
     gene            766..1170
                     /locus_tag="PLQ22_03615"
     CDS             766..1170
                     /locus_tag="PLQ22_03615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH59080.1"
                     /translation="MKTDQIKLEFQNEYKGKVIAPNGEIKIGRGENEYAPYHLLYGAL
                     GSCYYLTFLALCRKQKLEFDRVKITIDGEKHETSVALLKEVIVDVEIWSKDDKERITK
                     AAGQGAKYCSIYNTIQSIPAEVILKVKVYDEY"
     gene            1157..2398
                     /locus_tag="PLQ22_03620"
     CDS             1157..2398
                     /locus_tag="PLQ22_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59081.1"
                     /translation="MMSINYHLKGGYFSAKESLKYSFLKELYEFVLRKYPHAKLKDVD
                     FEDFFNYPPYLIGRYAGEYFLKETSSGYPENQSPKYFIGYCIENQLFIDLIEELIVFF
                     ALWRTTEGCKEDKAELFFANSWAALVDSAKYFYFKTKEELEASSEAPAIKKSEEIWDY
                     LSNIPEIYENQYKKDKAKGKVIPFKKNASFSGWYLDEECKVTFDGKVKQNLDLYPKWT
                     YSVTLCSNDGYKSFEELYDDLLNDFNKVNKLKLTKEIYETKEQGKIVELVYKSESYFD
                     KFFSNKQMYQKWEWLIRYLKSNILKKEVTSLLDYQEGKFGSEFRFRFELNSLILGRFS
                     KGKPKTVDYCGKGIKENLILKTNSCLKKVSYYSEMKLPLEFNNKKVKYWSKNPVNLEK
                     IMKIGYNNSNTILYAIYEEEL"
     gene            2395..3627
                     /locus_tag="PLQ22_03625"
     CDS             2395..3627
                     /locus_tag="PLQ22_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH59082.1"
                     /translation="MKDILLKEYAKLIVRIGANVQKDQIVVLNAPVEAHAFTKLVIEE
                     AYQAGAKKVVVNWSYGEDNKLYYQYASEETLKEFPEYNVKRLEYFMVNKAAQISVGSP
                     NPDLMKGVDPKKFVIASKAANEKIGFYRKYMMSNGSQWTIGIVPNLEWGKKVFPDVPY
                     YEVIEKLWGVILEACRVREGEDVVENWKKHHAQLAEHREKLNKYNFKSLHFKNKAGTD
                     LVVNLVEGHIWGGGSEKGQNGVTFSPNIPTEEIFTMPHSHGVNGKVVATMPLSYQGVL
                     IEDFWFEFKDGKVVDYGAKVGKEVLKTLVELDEGSSRLGEVALISHNSPISNMGILFY
                     NTLFDENASCHLALGNAYTMNIKDGYTLSEEELMKKGYNKSMAHVDFMFGSSDMEIIG
                     TTQDGKKVKVFTKGNFAF"
     gene            3631..>4151
                     /locus_tag="PLQ22_03630"
     CDS             3631..>4151
                     /locus_tag="PLQ22_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOH59083.1"
                     /translation="MKLFNFEGDYIGENKGLLDFFIKTLNGMAYGLFATLLVGTILTT
                     FATYVPLPEEVKKIILDVASVIKGLMGLGIGIGISMSLKLSGLKLIVASISGGIAVLF
                     PHVYADPSISLTLAGFLRLSSDPLVIYVVVIGTIFVMNTILTKQTPIDIIIVPILGVI
                     AAACFSLVVTKPIT"
BASE COUNT         1547 a          420 c          781 g         1403 t
ORIGIN      
        1 aaagaaaatg ccaagtggag aatcaagagc acaaatcttt catgtaagtg ataaagttgc
       61 atttgagaaa ttattcaaga aatatttttc taaatacttt attttattat caaaagaaga
      121 aataatgaaa ttatatggaa aaggtattcc tcataaaaca cttaaaacta gtttaggtga
      181 ttatgtcgct gttgctacta acaaatataa ttttgtagct aaagatggtg aaccaatgaa
      241 aggacatcat ggaggattta catataaaga aatgagaata ccgctaatta taggagttaa
      301 aaatggaata tagaacaatc ggttataaaa gttatggatt tattttattt tatggaatta
      361 ttgaagcttt tttgattact gctgttgtag tttttacaca gaagtatctt gaaactaaag
      421 agaatttagc aatcttaatc attgcaagtg ccttagtttt agttgcctta gttttattta
      481 tttttgaatt tcttgaacct aaaaagaaga ttcagattga tgaaaagtct ttaaaattaa
      541 gatttcgtaa taaagagact gtgattttat ttgatgattt tttagatgct aatatttatg
      601 gtaaatctgt tattagtaaa agtggtaaac ttaagatatt aactaaaaaa ggatttcatt
      661 ttgtagaaaa tgtggctgat gtagaagtgg taaagactgc tttacttcaa gcagcttata
      721 gttataaaat aaaagaaatt gaaagacaag aaggggagag taaaaatgaa gactgatcaa
      781 attaaattag aatttcaaaa tgaatataag ggaaaagtaa tagctccaaa tggagaaatt
      841 aaaattggac gaggtgagaa tgaatatgca ccataccatt tattatatgg tgctttagga
      901 agttgttatt atctaacatt tcttgcttta tgtagaaagc aaaaactaga atttgatcgt
      961 gttaagatta cgattgatgg tgaaaaacat gaaactagtg ttgccctatt aaaggaagtt
     1021 attgttgatg tagagatttg gtctaaagat gataaagaaa gaattacgaa agctgcaggt
     1081 caaggagcta agtattgttc tatttataat actattcaat ctataccggc tgaagttatt
     1141 ttgaaagtta aggtttatga tgagtattaa ttatcattta aaaggtggat atttttctgc
     1201 caaagaaagt ttaaaatatt catttttaaa agaattatat gagtttgttt taagaaaata
     1261 tcctcatgcg aaattgaaag atgttgattt tgaagatttt tttaattatc cgccttatct
     1321 aataggtaga tatgctggtg aatatttttt aaaagaaact agttcgggtt atccggaaaa
     1381 tcaaagccct aaatacttta ttggctattg tatagaaaat cagttgttta tcgatttgat
     1441 tgaagagtta attgttttct ttgctttgtg gcgtacaacc gaaggttgta aagaagataa
     1501 ggcagaattg ttttttgcta actcttgggc tgctttagta gatagtgcta aatactttta
     1561 ttttaaaact aaagaagagt tagaggcaag tagtgaagcg cctgcaatta aaaaatcaga
     1621 agaaatttgg gattacttga gtaatattcc ggaaatttat gaaaaccaat ataaaaaaga
     1681 taaagccaaa ggtaaagtta ttcctttcaa aaagaatgct tcattttctg gttggtattt
     1741 agatgaagaa tgcaaagtta cttttgatgg taaagtaaaa caaaatttag atttataccc
     1801 aaagtggact tattctgtaa ctctttgttc taatgatgga tataaatcat ttgaggaact
     1861 ttatgatgat ttattaaatg attttaataa agtaaataaa ttaaagctta caaaagaaat
     1921 ctatgaaaca aaagaacaag ggaaaattgt tgaactagtt tataaaagtg aaagttattt
     1981 tgataaattt tttagtaata agcaaatgta tcaaaaatgg gaatggttaa ttcgatattt
     2041 aaaatcaaat attttaaaga aggaagttac tagtttatta gattatcaag agggtaaatt
     2101 tggatctgaa tttcgattta ggtttgaact taattcactt attttaggac gctttagtaa
     2161 aggaaagcca aagacagtag attattgtgg taaggggata aaagaaaatt taatcttaaa
     2221 gactaatagt tgtttgaaga aagtatctta ttatagtgaa atgaaattac ctttggaatt
     2281 caataacaaa aaggttaaat attggagtaa aaatccggta aatcttgaaa aaataatgaa
     2341 aataggttat aataattcca atacaatatt atatgctatt tatgaggagg agttatgaaa
     2401 gatattttat taaaagaata tgcgaagttg attgtaagaa ttggagcaaa tgtccaaaag
     2461 gatcaaatcg ttgttttaaa tgcgccagta gaagcacatg catttactaa attagttatt
     2521 gaggaagcat atcaagctgg tgctaagaaa gttgttgtaa attggagtta tggtgaagat
     2581 aataaactat attatcaata tgcaagtgaa gaaactttaa aagaatttcc agaatacaat
     2641 gtaaagagat tagagtattt tatggttaat aaagctgcac aaatttcagt tggttcacca
     2701 aatcctgatt tgatgaaagg tgttgatcca aagaagtttg taattgcgag taaagctgct
     2761 aatgagaaaa ttggttttta ccgtaagtat atgatgagca atggttcaca atggacaatc
     2821 ggtattgtac ctaacttaga atggggtaaa aaagtattcc ctgatgttcc ttattatgaa
     2881 gtaattgaga aattatgggg agtaatttta gaagcttgcc gtgttagaga aggtgaagat
     2941 gttgttgaaa actggaaaaa gcatcatgca caattagctg aacatcgtga aaaattaaac
     3001 aagtataatt ttaagtcttt acatttcaag aataaagctg gtactgattt agttgtaaat
     3061 ttagttgaag gacatatttg gggtggcgga tcagaaaaag gtcaaaacgg agttacattc
     3121 tcaccaaata tcccaactga agaaatcttt acaatgccac atagtcatgg tgtaaatggt
     3181 aaagttgtag ccacaatgcc attaagttat caaggagttt taattgaaga tttttggttt
     3241 gaattcaaag atggtaaagt tgtagactat ggtgctaaag taggaaaaga agtattaaag
     3301 acattagttg aacttgatga aggttcatca agattaggag aagttgcttt aatttctcat
     3361 aattcaccaa tttctaatat gggaatttta ttctataata ctttatttga tgagaacgca
     3421 tcttgtcact tagctttagg taatgcttat acaatgaata ttaaagatgg ttatacttta
     3481 agtgaagaag aattaatgaa aaaaggttat aataaatcta tggcgcacgt tgactttatg
     3541 tttggtagta gtgatatgga aattattggg actacacaag atggtaaaaa agttaaagta
     3601 ttcacaaaag gaaatttcgc attttaaaag atgaaattat ttaattttga aggggactat
     3661 attggagaaa ataaaggact attagatttc tttattaaaa ccttaaatgg tatggcatat
     3721 ggtctcttcg ccaccttact tgtagggaca atcttaacta cttttgctac atacgtacct
     3781 ttaccagaag aagtaaagaa gattatttta gatgtagcat ctgttattaa aggattaatg
     3841 ggtttaggta ttggtattgg cattagtatg agtttaaaac ttagtggttt aaaactaatt
     3901 gtagcatcaa ttagtggtgg aattgctgtt ttatttcctc atgtttatgc tgatccaagt
     3961 atatctttaa cactagcagg atttttaaga ctttctagtg atcctttagt aatttatgta
     4021 gtagttattg gaacaatttt cgtaatgaat accatcctta caaaacaaac gccaatagat
     4081 attattatag taccaatttt aggagtaata gcagctgcat gctttagctt agtagttaca
     4141 aagccaatta c
//
LOCUS       DAOSGS010000079         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_19706_length_4112_cov_3.427902, whole genome shotgun sequence.
ACCESSION   DAOSGS010000079 DAOSGS010000000
VERSION     DAOSGS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19706_length_4112_cov_3.427902"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="PLQ22_03635"
     CDS             complement(<1..1946)
                     /locus_tag="PLQ22_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH59084.1"
                     /translation="MIREEYIEEIKKHILSARIEKVYNADLVVKNNGENSLIFPSFNQ
                     IIEYVNKASNVDKFFERINTTLDNYDKYVKGNNVSKIKVTLEPLEKRIKVSEETINKL
                     LDKFDESLNFQEFSKYDIKKITSLTFNDEYSDSLNFLFDLLVKLSKKVKDYIVKNSIW
                     NTAYKVDFEESIKDFKKDEPIKVLSTINDKEKKIIDKVYVPDFLSDGTEDLKEMMKSF
                     WLGLSALLYFSKPLEHVTYEVEVSTVKDIKDWLKDKVANHYVALDTLLSDQSTFKNYS
                     DIEIREIYEKINKIKIPKEINPRSESDMPNKKCKSEQRLAELIGLDSVKEAVDKIVAY
                     TKASKGSSSLNLHMAFYGNPGTGKTEVGKLIGEILYENGVLPKKVCIEATRSDLVGQY
                     IGETALKTQALIQKAMGGVLFIDEAYSLAIEGDSIDFGNEAVAELIKEMEDKKGKFCV
                     ILAGYKNKLSKMLATNPGFNSRIQFHLDFENFNRDEMCDIIKLMLDKDGYIVTEDALN
                     KMLDIVDVLRKNPDFANARDARNIIQQCVMCLNVRTGDPNDKTITLEDVNKYIQDAKL
                     NLPTGQSTKKVLSGEEELNELIGLENVKTSVKKIRAYAKKNKGASNLNLHMAFTGNPG
                     TGKTEVAHIISRVLYEAGVLPEAKV"
     gene            complement(1948..4038)
                     /gene="brxL"
                     /locus_tag="PLQ22_03640"
     CDS             complement(1948..4038)
                     /gene="brxL"
                     /locus_tag="PLQ22_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HOH59085.1"
                     /translation="MDELTNKILENFPGKVVRKDLTTMMKKGANVPTYVLEYLLGQYC
                     STDDENVISVGISKIKKVLTENYVRPDQSEYIKSKIRENGEYTIIDKISVVLNEREDK
                     YIAHFTNLKIDPFEVSPDLVIHNEKLLLGGIWCILRIAYQKKEEDEEEDLFGEKKKTK
                     KKSKFDSPFNIMSLKPIQMPNLDLNSIIEARKNFTKDEWIDLILRSAGYEPNELSKKQ
                     KFHYLLRMVPMVEKNYNLVELGPRGTGKSYIYSEISPYTILMAKGNTTVANMFYNMGR
                     RKVGLVGNWDCIAFDEVGGMAGATMEFVQTMKTYMANGNFALGVDSINADASIAFEGN
                     TFRTLADMIRTSHLFEPFPESFNNDSAFFDRINAYLPGWETPKLKESLFTKGFGLISD
                     CLSEYCHAMRKYDFTSNFMQYYSLNKNFNTRDDKAVTKTFSGLVKLVYPDEIMTKEDV
                     KELLEYAVEGRRRVKEQLKKMSPSEFNTVSLGFIDKETNEEIIVNVPEQPESTLVSEA
                     FEPAGFVYAIGRSIKDQIGVYKLENNVLAGQGKFDSKNIEGLGTNKTVKDSMNAAFVE
                     FGKHTKEVVAKSPNEYDYTLYFNDLQNKGVSDEVSVAEVVGLCSAIANVPVKPSMPIV
                     GRVVVSGTMMPLTTSLNDIVVAAVNVGAKKIMLPDECKGKFEALSVDTRKDLVAIFYS
                     TPIDAAKKALGLGD"
BASE COUNT         1324 a          751 c          460 g         1577 t
ORIGIN      
        1 actttagctt caggtaatac accagcttca tataggactc tagatataat atgagcaact
       61 tcagttttac ccgtacctgg attaccagta aatgccatgt gtaaatttag atttgaagct
      121 cctttattct ttttagcata tgctctgatc ttttttacac tagtctttac attctctaat
      181 cctatcaact cgtttagttc ttcttctcca gataaaactt tctttgttga ttgtccggta
      241 ggtaaattaa gtttagcatc ttggatatat ttattaacat cttctaatgt tattgtctta
      301 tcattaggat cacctgttct aacgtttaaa cacattacac attgttgaat aatatttcta
      361 gcatctcttg catttgcaaa atcaggattc tttcttaata cgtctacaat atctaacatc
      421 ttattcaatg catcttcagt gactatatac ccatctttat caagcattaa cttaataatg
      481 tcacacattt catctctatt aaaattttca aaatctaaat ggaattgaat tctagaatta
      541 aatcctggat tagtggctaa catttttgat agtttattct tatatcctgc taaaattaca
      601 cagaatttac ctttcttatc ttccatttct tttattagct cagcaactgc ttcattacca
      661 aaatctattg aatcgccttc aatagcaagt gaatatgcct cgtcaataaa taaaactcca
      721 cccattgcct tttgaattaa tgcttgagtt tttagtgctg tttcaccaat atattgacca
      781 accaaatcag atctagttgc ttcaatacat acttttttag gtaatacgcc attttcatat
      841 aaaatttcac caataagttt tccaacttca gttttacctg taccagggtt accatagaaa
      901 gccatatgaa gatttaaact actactacct ttagatgctt tagtatatgc aacaatctta
      961 tcaactgctt ctttaacact atctaaacca attaattcag ctaatctttg ctctgactta
     1021 cattttttat ttggcatatc agattcactt ctaggattaa tttctttagg aattttgatt
     1081 ttatttattt tttcatatat ttctcttatt tctatatctg aataattctt gaatgttgat
     1141 tgatctgaaa gcaaagtatc taatgctaca taatgattag caaccttatc ttttaaccaa
     1201 tctttaatat ctttaacagt gctaacctca acttcatatg taacatgttc taaaggtttt
     1261 gagaaatata gtaatgcaga taaaccaagc caaaaagact tcatcatttc ctttaagtct
     1321 tcagttccat ctgacaaaaa gtctggaaca tatactttat caatgatttt cttttctttg
     1381 tcattgattg ttgataatac ttttattggt tcatccttct taaaatcttt tatagattct
     1441 tcaaaatcta ctttataagc agtattccat attgaattct ttacaatgta atcttttact
     1501 tttttggata atttaacaag caaatcaaat aagaaattta gtgaatctga gtattcatca
     1561 ttaaaagtta gtgacgttat ttttttaata tcatacttac taaattcttg gaaatttaaa
     1621 gactcatcaa atttatccaa taacttattt atagtctctt cagatacttt gattcttttt
     1681 tcaagcggct ctagtgtaac tttaattttg ctaacattat tgcctttaac atacttatca
     1741 taattatcta aagttgtatt gattctttca aaaaacttat caacgttact tgctttgttt
     1801 acgtattcga tgatttgatt aaaagaagga aaaattaaag agttctcacc attattcttc
     1861 acaaccaaat cagcattata aactttttca attctagcag ataatatatg tttctttatc
     1921 tcttcaatat attcctctct aatcatatta atcccccaat cctaaggctt ttttagctgc
     1981 atctattggt gttgaataga atatagcaac caaatctttt ctagtatcaa ctgataatgc
     2041 ttcaaactta cctttacatt catcaggaag catgatcttt ttagcaccaa cattaactgc
     2101 agcaacaaca atatcattta aactagttgt taaaggcatc attgtacctg aaactacaac
     2161 tctgccaaca attggcatag atggtttaac aggaacgttt gcgattgcag agcataagcc
     2221 aacaacttca gcaacagata cttcatcact tacaccttta ttttgtaaat cattgaaata
     2281 taatgtatag tcatactcat ttggagattt agctactact tcttttgtat gcttaccaaa
     2341 ttcaacaaat gcagcattca tactatcttt aactgtttta tttgttccta aaccttcaat
     2401 atttttagaa tcaaattttc cttgaccagc aagaacatta ttctctaatt tatatacacc
     2461 aatttgatct ttaattgatc taccaattgc atatacaaat ccagctggtt caaacgcttc
     2521 tgaaactaat gttgattcag gttgttctgg aacattaaca attatttctt catttgtttc
     2581 tttatcaata aatcccaaac ttactgtatt aaattcagat ggactcattt tcttaagttg
     2641 ttctttaact cttcttctac cttcaactgc atattctaat aattctttaa catcttcttt
     2701 tgtcataatt tcatcaggat atacgagttt tacaagtcca gagaaagttt ttgtgacagc
     2761 tttgtcatca cgagtattaa agttcttatt taatgagtaa tattgcatga aattactagt
     2821 aaaatcatat tttctcattg cgtgacaata ctctgatagg cagtctgaaa taagaccaaa
     2881 tcctttagta aataagctct cttttagttt aggcgtttcc caaccaggta aataagcatt
     2941 aattctatca aagaatgcag aatcattatt gaaactttct gggaatggct caaataaatg
     3001 tgaagttcta atcatatcag ctaaagttct aaatgtatta ccttcaaaag caattgaagc
     3061 atcagcgtta atagaatcaa cgcctaaagc aaaattacca tttgccatat atgttttcat
     3121 tgtttgaaca aattccattg tagcaccagc cataccacca acttcatcaa aagcaataca
     3181 atcccagttg ccaactaaac ctacttttct tctacccatg ttatagaaca tgttagcaac
     3241 tgtagtattg cctttagcca ttaaaatggt atatggtgat atttcgctat aaatatatga
     3301 ttttcctgtc ccacgaggac ctaattcaac taagttataa ttcttttcaa ccattggaac
     3361 catacgtaac aaataatgga acttttgttt tttactcaat tcattaggtt catacccagc
     3421 agaacgtaaa attaaatcaa tccactcgtc tttagtaaaa ttctttcttg cttcaataat
     3481 agaattcaaa tctaaattag gcatttgaat aggttttaaa gacatgatat taaatggtga
     3541 atcaaattta gatttctttt tggttttctt cttttcgcca aataaatctt cctcttcatc
     3601 ttcttctttc ttttggtaag cgattcttaa aatacaccaa atacctccta aaagaagttt
     3661 ttcattatga ataactaaat caggagaaac ttcaaatgga tcaatcttta aattagtaaa
     3721 atgagcaata tacttatctt ctctttcatt taaaactaca gagattttat caataatagt
     3781 atattcaccg ttttctctaa ttttagattt aatatattca ctttgatctg gtctaacata
     3841 attttcagtt aggacctttt ttattttaga aataccgaca ctaataacat tttcgtcatc
     3901 agttgaacag tattgtccta ataaatattc aagtacatat gtaggaacat tagccccttt
     3961 tttcatcatt gtagttaaat cttttctaac tacttttcca gggaaatttt ctaatatttt
     4021 atttgttaat tcatccatcg tttatccctc ctaaaaatca aagtctccac caaatgaatt
     4081 ctcaatctta aattcaactt tcatcaaagt tt
//
LOCUS       DAOSGS010000080         4017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_20375_length_4017_cov_3.942706, whole genome shotgun sequence.
ACCESSION   DAOSGS010000080 DAOSGS010000000
VERSION     DAOSGS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20375_length_4017_cov_3.942706"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLQ22_03645"
     CDS             complement(<1..657)
                     /locus_tag="PLQ22_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOH59086.1"
                     /translation="MESIRKIFQVGYGPSSSHTIGPTKAATIFKEKNKEADKFTVDLY
                     GSIALTGKGHLTDKALYRVFGEDLEIKFDFDTHYDFHPNGLKFYAYKEGKLIDEWLIF
                     SIGGGELKELGHKETIDCKEIYKQTTFNEILEYCKEHHLSLFEYIIKHEDKDILEFAK
                     GILKAMFDSVERGRNKEGKLSAQNKLFVKRKSKEYYDKYKKSGDFDVLVMSNALAVAE
                     E"
     gene            781..1446
                     /locus_tag="PLQ22_03650"
     CDS             781..1446
                     /locus_tag="PLQ22_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOH59087.1"
                     /translation="MQKVIAVTPRIIYEGTTAKEFVNRRYVRALLNRGFLVIMLTLEN
                     ENTESLLEKCDGFLITGGDDIDPVHFGEVNTGESKDVMKELDDLDKIVVEYALKSKKP
                     LLGICRGHQSINVFAGGSLYQDIGKSHSGLKDGHIVHTVPNRLLNFKKEINVNSYHHQ
                     ALKRIAPDFIEIAKHEDGTNEAIIHKTLPIIGIQWHPEMQQDNEPSKIIFDTFAKLVN
                     NHK"
     gene            complement(1462..2028)
                     /locus_tag="PLQ22_03655"
     CDS             complement(1462..2028)
                     /locus_tag="PLQ22_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOH59088.1"
                     /translation="MKEKKIIYSREEMFKMNKKKLEERGVTLEDIALIAYKQQSKYNP
                     NIQLSTCLESVAKILTYRDIFHIVQLGIELDILTEQGKLSEPMQGILEEDLGMFGIDE
                     LLGLSIASNYGVIGQTNFGDIDVNKPGIVGKLNKEGKGGKCHTFLDDIVGALAAAAST
                     RVAQIQSEEVAEHDPNIKQLQMDLKQKK"
     gene            complement(2025..2954)
                     /gene="fmt"
                     /locus_tag="PLQ22_03660"
     CDS             complement(2025..2954)
                     /gene="fmt"
                     /locus_tag="PLQ22_03660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH59089.1"
                     /translation="MKIIFMGTPEFAVPVLEEINKHFEVVLVVTQPDSRIRKKIVYSE
                     VKMKALELGLPVFQPLNINKEKDYILNTECDLIVTAAYGQFLGKAILNHPKIAAINCH
                     GSLLPKYRGGAPIQRAIMNGEKEIGVSIIYMTAKMDAGNILAKKGISLPLEWGEKEAF
                     DALSNLGAKLTVHVINQLAKEKKPGEIQNEEEVTFAPIISNEDEFISFEKEAYKVYNK
                     IRAINYSKGAYFNFKTEVYKIYQAKLYLNKKGNPGEIIEITKDEIIIACKEGAISLLS
                     IRRQGKNTTSVRDFLNGVGKEIFKVGEIIGGER"
     gene            complement(2965..3813)
                     /locus_tag="PLQ22_03665"
     CDS             complement(2965..3813)
                     /locus_tag="PLQ22_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59090.1"
                     /translation="MCNNCSEVIKCPSCKISLSYNKNKNELSCNYCDYKVSYSNLKCP
                     SCNSSKLSFFNLGLESLEEDLKKFYPDKKYLLITAETMSNYSKYSEAVLTIEEQAVDF
                     IIGTNLVFDLLNYPNISFVALINSDNILNYGDYRAAETLFLNIVKVLNKDNLGLILQG
                     YNLDHYSITLGLKNDYQNFYFQEIRQRNALNYPPKLEIMRLIIGGDYSKRYHAAYYFR
                     KVFRSLFTDSEILGPKYTRKYQGMQLIIKTNDYEKLSKLIDEVKEKFKKDNLEFIFER
                     HPKTLY"
BASE COUNT         1366 a          686 c          452 g         1513 t
ORIGIN      
        1 ctcttccgct acagctaaag cattactcat aactaaaaca tcaaaatccc ctgatttttt
       61 atatttatca taatactctt tagactttct cttcacaaat aatttatttt gagctgataa
      121 ttttccttct ttatttcttc ctctttcaac actatcaaac attgctttta agataccctt
      181 cgcaaactct aaaatatctt tgtcttcatg cttgataata tattcaaata atgataaatg
      241 atgttctttg caatattcta gaatttcatt aaaggtagtt tgcttgtata tttctttaca
      301 atcgatcgtt tctttatgac ctagctcttt taattctcct ccaccaatag aaaaaattaa
      361 ccattcatca attaatttac cttctttgta tgcataaaac tttagtccat taggatgaaa
      421 atcatagtga gtatcaaaat caaatttaat ttctaaatct tcaccaaaaa ctctatataa
      481 agctttatca gttaaatgac cttttcctgt taaggcaatt gaaccatata aatcaactgt
      541 aaatttatca gcttctttat ttttttcttt aaagattgtg gctgctttag taggcccaat
      601 cgtatgtgaa cttgatggtc cataacctac ttgaaatatt tttctaatac tttccataac
      661 aatacctctt gtttattatt ataccaatta tgctttcatt ttgccattaa aaaacttgat
      721 tttaagatat atttataata taattatatt atacttattt aaggaggtag atttaagatt
      781 atgcagaaag ttattgctgt aactccaaga attatttatg aaggtacaac agctaaagaa
      841 tttgtaaacc gccgttatgt tagggcgtta ttaaacagag gatttttagt aatcatgctt
      901 actttagaaa atgaaaacac tgaaagctta ctagaaaaat gtgatggatt tttaataaca
      961 ggtggagatg atattgaccc agttcatttt ggtgaagtaa atactggaga atcaaaagat
     1021 gttatgaaag aactagatga tttagataag attgttgttg aatatgctct aaaaagcaaa
     1081 aaaccacttt taggtatttg tcgaggtcat caatcaatta atgtttttgc tggtggaagc
     1141 ttataccaag atatcggtaa aagtcattct ggtcttaaag atggtcatat tgtccacaca
     1201 gttccaaata gattattaaa ctttaaaaaa gaaataaatg taaatagcta tcatcaccaa
     1261 gcattgaaaa gaatcgcacc agattttatt gaaattgcta aacatgaaga tggtacaaat
     1321 gaagctatta tccataaaac actgccaatt attggaattc aatggcaccc tgaaatgcaa
     1381 caagataacg aacctagtaa aatcatcttt gatacatttg ctaagttagt taataaccat
     1441 aaataaaaaa gtgagttaat ctcacttttt ttgttttaaa tccatttgta attgtttgat
     1501 attaggatca tgttctgcta cttcttcact ttggatttgg gcaactcttg tactggcagc
     1561 tgcagccagt gcacctacaa tatcatctaa aaaagtatga cattttccgc ctttaccttc
     1621 tttgtttaat tttccaacaa tccccggttt atttacatca atatctccaa aattagtttg
     1681 accaattaca ccataattag aagcaatact taaacctaaa agctcatcaa ttccaaacat
     1741 gccaagatct tcttctaaaa ttccttgcat tggctcagat aatttccctt gttcagtcaa
     1801 aatatctaat tcaataccaa gttgtacaat atggaaaata tcacgataag ttaatatctt
     1861 cgctacactt tccaaacaag tacttaactg aatattagga ttatactttg attgttgttt
     1921 ataagcaatt aaagcaatat cttctaatgt tactcctctt tcttcaagct tctttttatt
     1981 cattttaaac atttcttcac gagaataaat aatctttttc tctttcatct ttctcctcct
     2041 ataatttctc caactttaaa aatttctttt cctacaccgt ttaagaaatc tctaactgaa
     2101 gttgtattct taccttggcg tctaattgat aataaactaa ttgcaccttc tttgcaagca
     2161 ataattattt catctttggt aatctcaata atctctccag gattaccttt tttatttaaa
     2221 tacagtttag cttgataaat tttataaact tctgttttaa aattaaaata agcaccttta
     2281 gaataattaa tagctcttat cttattatac accttataag cttctttttc aaaagaaata
     2341 aattcatctt cattacttat aatcggtgca aaagtaactt cttcttcatt ttggatttct
     2401 ccaggttttt tttctttagc taattgatta attacatgaa ctgttaactt agcacctaaa
     2461 ttacttaaag catcaaatgc ttctttttct ccccattcta aaggtaaaga aattcctttt
     2521 ttagctaaga tattaccagc gtccatcttt gcagtcatat aaataatact aacaccgatt
     2581 tctttttccc cgttcataat tgctctttga attggtgctc cacctctata tttaggaagt
     2641 aatgacccat gacaattaat tgctgcaatc tttggatgat ttaaaattgc ttttcccaaa
     2701 aattgaccat aggctgcagt aacaattaaa tcacattctg tatttaatat ataatctttt
     2761 tctttattga tattaagagg ttgaaaaact ggcaagccta actctaaagc tttcattttt
     2821 acttcgctat aaacgatttt ttttctaatt cttgaatcag gctgtgtcac tactagcacc
     2881 acttcaaaat gcttatttat ttcttctaaa actggaacag caaattctgg tgttcccata
     2941 aatataattt tcaaatcatt cctcctaata aagagttttt ggatgtcttt caaatataaa
     3001 ctctaaattg tcttttttaa acttttcttt tacttcatca attaatttac ttaacttttc
     3061 ataatcatta gttttgatta ttaattgcat accttgatat tttcttgtat atttaggccc
     3121 taatatttca ctatcagtaa aaagacttct aaatacttta cgaaaataat aagctgcatg
     3181 atatctctta ctataatcac caccaataat taatctcatt atctctaatt ttggtggata
     3241 atttaaagca ttcctttgcc ttatctcttg aaaataaaag ttttgataat cattttttaa
     3301 tcctaaagtg atactataat gatctaaatt atatccttgt aagattaaac ctaaattatc
     3361 tttatttaag acctttacaa tatttaaaaa cagagtttct gcagctcggt aatctccata
     3421 atttaaaata ttatcagagt taattaaagc tacaaaacta atattaggat aattaagtaa
     3481 atcaaaaact aaattagtcc caataataaa atctactgct tgttcttcaa tcgttagtac
     3541 tgcctcacta tatttactat agttactcat tgtctcagca gtaatcaata aatacttttt
     3601 atccggataa aatttcttta aatcttcttc caaactttct agccctaagt taaagaaact
     3661 taacttgcta ctattacaac taggacattt taaattagaa taagatactt tataatcaca
     3721 ataattacaa cttaattcat tcttattttt attataagat aaacttatct tacaactagg
     3781 acacttaata acttcagagc aattattgca cattaccatt tcactataat ttttttgatt
     3841 caaaaggagg acagaaaata tgcaattccg acagcaaaag tatttgcttg aataaaagca
     3901 gtattgcaaa actcagtttt aaaattgatt caggctgttc tacaataagt ttcgattacg
     3961 aacaggaata ctcttcaaat tgtgacctta ttttagaagt aaataatcag ataatag
//
LOCUS       DAOSGS010000081         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_20776_length_3967_cov_5.146472, whole genome shotgun sequence.
ACCESSION   DAOSGS010000081 DAOSGS010000000
VERSION     DAOSGS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20776_length_3967_cov_5.146472"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLQ22_03670"
     CDS             complement(<1..440)
                     /locus_tag="PLQ22_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOH59091.1"
                     /translation="MSKKKKPNKDSKKRTKQIDLDNQILLSESKKYEKQLRKEAKAEA
                     KALAKVKRLRKKKGYIVNEEDIVRFDVDLKVGLTDEQVEQREKEGLANKPDAQAGKSV
                     AMIFVTNIFTFFNMLFFGIAIVLIVIEEYTNLLFLGTVLSNMIIG"
     gene            complement(450..1001)
                     /locus_tag="PLQ22_03675"
     CDS             complement(450..1001)
                     /locus_tag="PLQ22_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOH59092.1"
                     /translation="MKGLILLANGFEEAEAIITIDILRRAKLDVTLASVGLDLTVTSS
                     HNIQVLADTRLSDVISEEYGFLIIPGGLKSVLTFHSSSLVSEIVEEFVLNQKIIGAIC
                     AGPTVLSRLGLLKGKKFTCFPGLDEYLKSGAYHPEKEVIVANNIITARAMAYTIEFAL
                     EIVKKISGKEEAKRIEKAITGMR"
     gene            complement(998..1513)
                     /locus_tag="PLQ22_03680"
     CDS             complement(998..1513)
                     /locus_tag="PLQ22_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HOH59093.1"
                     /translation="MKIYIASQNKTKVEAVKLVFKEASVIALKTDSKVSDQPKSEKET
                     MLGAYNRAKSLPKDGLRIGLEAGVSLEEDILFLINWGVLIDQDDNVYLASGTKIPLPK
                     FIYNEIYNQGKELADIMNYHYNKEEINTKEGAIGIFTNDNVQRIEIFIHIVKLLKGQY
                     ETKNYVGRINK"
     gene            complement(1510..3201)
                     /gene="trmB"
                     /locus_tag="PLQ22_03685"
     CDS             complement(1510..3201)
                     /gene="trmB"
                     /locus_tag="PLQ22_03685"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH59094.1"
                     /translation="MRVKNIKDADIKLLEYKDYYLEKSNEYKGKWKELFKNNNPIHLE
                     IGMGKGKFLKESAKLNPNINYIGIEKSSTILLKAVRMISTSKLKNIYLLNIDASNLLD
                     YFASSEISHIYLNFSDPWPKDRHAKRRLTAPSFLRKYQDILVNNGVIEIKTDNENLFN
                     YSLETLEEAKFLIKEQSSNFDSKKRNIVETEYEQKFKESNKTIYYIKVVKMTTKKRMK
                     LYEDLANLHGPSGDEGQVRDYLLKVYRAEADEILRDGLGSVFGMKKGKKDEPVIMIAG
                     HMDEVGGMVTRLQENGFVKFINIGGVVPNVWVSQNVVIQTAKGKVKGVVGSVPPHMGA
                     AGEITVDSLFIDVGAESKKDLEKYGIKPGDFLTFENDYYVTKNKNRIVSKAWDNRFGT
                     GMALEVFRELKNIEHPNTVICGATVQEEVGLRGAKTSSQMVKPDLFIAIDVSPVGDAT
                     PGPGDSFGKLNGGFLIRFYDPGNIMNPKMLEYVEGLAKKHKIDYQFFLSKGGTDAAIA
                     QYAGSGCFSITIGLPGRYIHSNTSMIDVRDVEAVKAIVLEIIKDFSKERLLELKQ"
     gene            complement(3203..>3967)
                     /locus_tag="PLQ22_03690"
     CDS             complement(3203..>3967)
                     /locus_tag="PLQ22_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH59095.1"
                     /translation="DEIVSFSAGDRYNVVPDKAEALIKKNLEVEFLNYLKENDLKGEV
                     SKEDKLCKLTIYGKTAHGSAPYLGVNAAIKLSCFLSDYFTNSVVNFVSDKMRDPYFRG
                     MSLDYENNKMGKLTVNLAKIEINSKGNKIGLNLRYPIDWDKEEFLKKLHLQIGAYGFK
                     FEILSDSNPHYVDPEEDLVQTLYNSYKKYTNDEESKPYTIGGGTYARILKKGVSFGPT
                     FPHTIANIHQPNEYITVEDCLLSTIIYADAIKELSK"
BASE COUNT         1272 a          756 c          394 g         1545 t
ORIGIN      
        1 ccaataatca tattagataa aacagttcct aaaaataaca aattagtata ttcctcaatt
       61 acaattaaaa caatagcaat tccaaaaaac aacatattaa aaaaagtaaa aatatttgtt
      121 acaaaaatca tagctacaga cttaccagct tgagcatctg gtttattggc tagtccttct
      181 ttttctcttt gctctacttg ttcatcagtc aatccaactt ttaaatcaac atcaaaacgg
      241 actatatctt cttcattgac aatataacct tttttctttc ttagcctttt aacttttgct
      301 aaagcttttg cttctgcttt tgcttctttt ctcaattgtt tctcatattt tttgctttca
      361 gatagtaata tttgattgtc taaatctatt tgtttagttc ttttttttga atccttatta
      421 ggcttctttt ttttagacat aatagcctcc tatctcatgc cagtaattgc tttttctatt
      481 ctttttgctt cttcttttcc tgatattttt ttcacaattt ctaaagcaaa ttcaattgta
      541 taagccattg ctctagcagt aataatgttg tttgcaacaa taacttcttt ttctggatgg
      601 tatgctccac tctttagata ttcatctaaa ccaggaaaac aagtaaactt ctttcctttt
      661 aataatccta atctacttaa aacagttgga ccggcacaaa tagcaccaat aattttttga
      721 tttaaaacaa actcttccac aatctcgcta actaatgaac tagaatgaaa agtcagtact
      781 gattttaaac caccagggat tattaaaaaa ccatattctt cgctaattac atcacttaat
      841 cttgtatcag ccaatacttg aatgttatgg cttgaagtaa cagttaaatc taatcctaca
      901 cttgctaaag tcacatctaa tttagccctg cgtaaaatat caatcgttat gattgcttca
      961 gcttcttcaa agccattcgc taataaaatt aatcctttca tttattaatc ctccctacat
     1021 aatttttagt ttcatattgt ccttttaata gtttaactat atgaataaat atttcaattc
     1081 tttgaacatt atcgttggta aaaataccaa tcgcaccttc cttagtattt atttcttctt
     1141 tgttataatg ataattcata atatctgcta attctttacc ttgattataa atctcattat
     1201 aaataaactt aggtaaagga attttagtgc ctgatgcaag ataaacatta tcatcttgat
     1261 caattaaaac tccccaatta attagaaata agatatcttc ttctaaacta acaccagcct
     1321 ctaaaccaat tcttaatcca tcttttggta atgatttagc tctgttataa gcacctaaca
     1381 tcgtttcttt ttcactttta ggttggtcag ataccttact atctgttttc aaagcaataa
     1441 ctgaagcttc tttaaaaact aattttacag cttctacttt tgttttattt tggctagcga
     1501 tatatatttt cactgtttta attcaagtaa tctttcttta ctaaaatctt taataatttc
     1561 taaaacaatt gcctttaccg cttctacatc tctaacatca atcattgatg tattagaatg
     1621 aatgtatctt cctggcaacc caattgtaat actaaaacaa ccactacctg catattgagc
     1681 aatagctgca tcagttccgc cctttgataa gaaaaattga taatcaatct tatgtttctt
     1741 agccaatccc tctacgtact caagcatctt tggattcata atattacctg gatcataaaa
     1801 tctaattaaa aatccaccat ttaatttacc aaaagaatct cctggtcctg gtgtagcatc
     1861 accaactgga gaaacatcaa ttgcgataaa caagtctggt tttaccattt gtgatgatgt
     1921 cttagcgcct cttaacccaa cttcttcttg aactgtggct ccacaaatta cagtattagg
     1981 atgttctata tttttaagtt ccctaaatac ttctaaagcc atacctgtac caaaacgatt
     2041 atcccatgct ttagatacaa ttctattttt gtttttagtt acataataat cattttcaaa
     2101 agttaagaaa tctcctggtt taattccata tttttctaaa tctttctttg attcagcacc
     2161 aacatcaata aataaactat caacagtaat ttcaccagct gcacccatat gaggtggtac
     2221 agaaccaact acgcctttaa ctttaccttt agctgtctga atcactacat tttgtgatac
     2281 ccaaacatta ggaacaacac cacctatatt aataaacttc acaaaaccat tttcttgaag
     2341 tcgagttacc atacccccaa cttcatccat atggccagca atcataatta ctggttcatc
     2401 ttttttacct tttttcatac caaaaacaga gcctaatcca tctcttagaa tttcatctgc
     2461 ttctgctcta tatactttta ataaataatc tctaacctgc ccttcatcgc cacttggacc
     2521 atgcaaatta gctaagtctt catataattt cattctcttt ttagtcgtca tttttaccac
     2581 ctttatataa tatattgttt tattactttc tttaaatttt tgttcatatt ctgtttcaac
     2641 tatatttctt ttctttgaat caaaattact actttgttct ttaattagaa acttcgcttc
     2701 ttctaatgtt tctaaactat aattaaataa attttcatta tctgtcttta tttcaataac
     2761 tccattattt actaaaatat cttgatattt tcttaaaaaa gatggagccg ttaatcttct
     2821 ttttgcatgt ctatctttag gccaagggtc agaaaaattc aaatagatat gtgatatttc
     2881 agaactagcg aaataatcta ataaattact tgcatcaata tttaataaat aaatattttt
     2941 aagtttactt gtactaatca ttcttactgc ttttaaaaga atagtacttg atttttcaat
     3001 tccaatataa ttaatattag gatttaactt ggcactttct tttaaaaact ttcccttacc
     3061 catcccaatc tctaaatgaa ttggattatt atttttaaaa agttctttcc attttccttt
     3121 atattcatta gatttttcta aataataatc tttatattct aagagtttga tatctgcatc
     3181 tttaatattt ttaactcgca cactatttac ttagctcctt aattgcatcg gcgtaaataa
     3241 ttgtactaag caaacaatct tcaactgtta tatattcatt aggttgatga atattggcta
     3301 ttgtatgagg aaatgttggt ccaaaagaaa cacctttctt tagtatcctt gcatatgttc
     3361 ctccaccaat agtataaggt ttactttcct catcattagt atatttctta tatgaattat
     3421 aaagtgtttg aactaaatct tcttctggat ctacataatg aggattacta tcacttaaga
     3481 tttcgaattt aaaaccatat gctccaattt gcaagtgaag tttctttaag aactcttctt
     3541 tatcccaatc aattggatat cttaaattca aaccaatttt atttccttta gaattaattt
     3601 caattttagc taaatttact gttaacttac ccattttgtt gttttcataa tctaaactca
     3661 ttcctctaaa ataaggatct cgcattttat cactaacaaa atttacaact gaattcgtaa
     3721 agtaatcact taagaaacaa gataatttaa tagccgcatt tactcctaaa tatggagcac
     3781 taccatgagc tgttttacca taaattgtta atttacacag tttatcttct ttactaactt
     3841 cacctttaag atcattttct tttaaataat ttaaaaactc tacttctaaa tttttcttaa
     3901 tcaaagcttc tgctttatca ggaacaacat tataacgatc tccagctgaa aatgaaacaa
     3961 tttcatc
//
LOCUS       DAOSGS010000082         3923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_21099_length_3923_cov_4.105998, whole genome shotgun sequence.
ACCESSION   DAOSGS010000082 DAOSGS010000000
VERSION     DAOSGS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21099_length_3923_cov_4.105998"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="rsgA"
                     /locus_tag="PLQ22_03695"
     CDS             complement(<1..470)
                     /gene="rsgA"
                     /locus_tag="PLQ22_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HOH59096.1"
                     /translation="MEGIVTKLVSGDYTVRVKDKEYICKPRGVFRHKVQDIYVGDRVI
                     IDEDNIIIEVLERKNLLLRPSVANIDQVILVFSLKEPDLNLHLLDKMIAYYEYLDLKI
                     TIVFSKTDLDEDKSYLDIVNYYKNLYKVYFFSKHEENDFSGLFTDKISVLSGQSG"
     gene            complement(470..1504)
                     /gene="rlmN"
                     /locus_tag="PLQ22_03700"
     CDS             complement(470..1504)
                     /gene="rlmN"
                     /locus_tag="PLQ22_03700"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH59097.1"
                     /translation="MRSLYSISKDNLVSFLVENGHPKYRAVQIYEGLYSQNVSSIDDI
                     KVLKQEFKDFLKGHFYLNSIELVKKEESVDGTVKYLFRLYDGHLIETVLMKHDYGNSV
                     CVSIQVGCNFACAFCASGLLKKQRNLRVDELVLQIKYINDDLSKRCEKVSSVVVMGIG
                     EPFDNYDNLLEFLEIINDDKALAIGSRHITVSTVGLAPKIEEFSNFPLQVNLAISLHF
                     ASDEKRSKYMPVNRAYNLKVLKDAIIKYYEKTNRRITIEYILLKDVNDTLEDARALVE
                     FTSGLNCYVNLIPYNSTISEFCRSDEKVVNEFFKYLKRNNVNVTKRREQGHDINAACG
                     QLRNKQLKEG"
     gene            complement(1501..2580)
                     /locus_tag="PLQ22_03705"
     CDS             complement(1501..2580)
                     /locus_tag="PLQ22_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH59098.1"
                     /translation="MNILKIAVDIITKVMNKDFSLNREFKKTYAKYQFTEEEKIRLED
                     LILGVIDHYLELEYYLEPYLYKKTKDETKNLLLIAIYLLIYRQENLELVIEEVCKVAV
                     LKNKGVSNFVKRTLRNFSKNRTRDLGNLIAIDRLSIEYSYPLWLVSYLLKDYSYRDVA
                     KILKQEDLKGKNLTKMAIRQLKLEDQKIGLDLWASRESSSLLSDKLKKGKLYAVLDQK
                     KKQKQLKRYLSKMNKKNVKPVLISPLVLQDAIKDDSLDFVLVNAPSSNLVNYEKEKDL
                     KYKLSLSGIKEIVSIQKAVLDEISALLKVGGEFVYYTNTVNKDENERVIISFLENNPN
                     YKLIKEVSYDNSEVIGYIAYLRKVA"
     gene            complement(2577..3389)
                     /locus_tag="PLQ22_03710"
     CDS             complement(2577..3389)
                     /locus_tag="PLQ22_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOH59099.1"
                     /translation="MRGDFHMHTTHSDGSLSVEEILEELQKQKIKYFSVTDHDCIAGT
                     VEAVALAPKFDLVGIYGLELSTINNNESIHILGYFSKKEDILKIEKFLKHMQEVREKR
                     AKEIQRRLKEYFNIEIDLGKLDPKVTITRYNIASLIIEAGYPYEREEIFENFIGDNCP
                     AYIPSTKISSKEGIKVIKDAGGIAILAHPILYKKNKVEEFLEYGIDGIEVFYPNATDK
                     QIIDNLALCKRHNLLVTAGSDFHRFDDYKHGDIGSVSLKKPYLTNFLERLNK"
     gene            complement(3392..>3923)
                     /locus_tag="PLQ22_03715"
     CDS             complement(3392..>3923)
                     /locus_tag="PLQ22_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59100.1"
                     /translation="NVEEVDVIIAKISACEKEFKEANAILNYLYDAYFRNIKMTSLAA
                     IKDKKAYELEGLAREVNDLIKNYNNLEHAYYDIYYYSGDLIVKTVKALVNCLEKGKNK
                     SYATIYNYSYFLKSDAILNFEFKEWIALFNKFKFIMKTTSNVELYDYLQFSELYQKLE
                     KRYFVILIFYELGGGR"
BASE COUNT         1349 a          698 c          361 g         1515 t
ORIGIN      
        1 ccactttgac cacttagaac agaaatttta tccgtaaata atccactaaa atcattttct
       61 tcatgtttag agaaaaagta aactttatac aaatttttat aataattaac tatatctaaa
      121 tagcttttat cttcatctaa atctgttttt gaaaagacaa ttgtaatctt taaatctaaa
      181 tattcataat aagcaatcat cttatcaagc aaatgtaaat taaggtctgg ttcttttaag
      241 gaaaatacta aaatgacttg atcaatgtta gctacacttg gacgtaaaag caggtttttt
      301 cgttctaata cttcaataat tatgttatct tcatcaataa taactctatc gcctacataa
      361 atatcttgca ctttatgacg aaaaacaccc ctaggcttac aaatatattc cttatcctta
      421 actcttactg tataatcacc acttacaagc ttagttacaa taccttccat taaccctcct
      481 taagttgttt gtttcttaac tgaccacaag ctgcattaat atcatgccct tgttctcttc
      541 ttttagttac atttacatta tttcttttta aatatttaaa aaactcattt actacttttt
      601 catcacttct acaaaactcg ctaatagtac tattataagg aattaaattt acataacagt
      661 ttaatccaga tgtaaattct actaaagctc ttgcatcttc taaagtatcg ttaacatctt
      721 taagtaaaat atactcaatt gtaatcctac gattagtttt ttcatagtat ttaataatag
      781 catcttttaa tacttttaag ttgtaagctc tatttactgg catatactta cttctcttct
      841 catctgatgc aaaatgaagc gaaattgcaa ggtttacttg taatggaaaa ttactaaact
      901 cttcaatttt aggcgctaaa cctacagtac ttaccgtaat atgacgcgaa ccaattgcaa
      961 gtgctttatc atcattgata atttctaaaa actctaataa attgtcataa ttatcaaatg
     1021 gttcacctat ccccataact acaacgctac ttactttttc gcacctttta cttaaatcat
     1081 cattaatata cttaatttgt aagactaatt catctactct taagtttctt tgcttcttta
     1141 aaagtccaga tgcacaaaaa gcacaagcaa aattgcaacc tacttgaata gaaacacaaa
     1201 cactattacc ataatcatgt ttcattaata cagtttcaat taaatgacca tcatataatc
     1261 taaacaaata cttaacagtt ccatcaactg attcttcttt cttcacaagt tcgatactat
     1321 ttaaataaaa atgccctttt agaaagtctt taaactcttg ctttaaaact ttaatatcat
     1381 caatagaact aacattttgg ctatataaac cttcatagat ttgcacagct ctatacttag
     1441 gatgtccgtt ttcaactaaa aatgatacta aattatcttt tgaaatacta tataacgatc
     1501 tcatgctact ttccttaagt aagctatata accgattact tcactattat cataacttac
     1561 ttctttaatt aatttataat ttggattatt ttctaaaaag ctaataatta ctctttcatt
     1621 ttcatcttta ttgacagtat tagtataata aacaaactct ccaccgactt taagtaaagc
     1681 agaaatttca tctaaaacag ctttttgaat agaaacaatt tcttttattc ctgataaact
     1741 caatttatat tttaagtctt tctctttttc ataatttact aagtttgaag aaggtgcatt
     1801 tactaaaaca aaatctaaag aatcatcttt aattgcgtct tgcaatacta aaggagaaat
     1861 taaaacaggt tttacgttct ttttattcat cttacttaaa taacgcttta gctgtttttg
     1921 tttcttcttt tgatctaaaa cagcatatag cttacctttt ttcaatttat cgcttaaaag
     1981 actactgctt tctctactag cccaaagatc taacccaatt ttttggtctt ctaattttaa
     2041 ttgtctgata gccattttag ttaagttttt cccttttaaa tcttcttgtt ttaagatttt
     2101 agctacatca cgataactat agtcttttaa taaataactt actaaccata aaggataaga
     2161 atactcaatg cttaaacgat caatagcaat taaattacct aaatccctcg tcctattttt
     2221 agaaaagttt cttaaagttc tctttacaaa attactaact cctttatttt ttaaaacagc
     2281 aactttacat acttcttcaa taactaattc taaattctct tgacgataaa ttagtaaata
     2341 aatagcaatt aatagcaagt ttttagtttc atctttagtt tttttatata aataaggttc
     2401 taaataatat tctaattcta aataatgatc aattacccct aaaataagat cttctaatct
     2461 aattttttct tcttcagtaa attgatattt tgcatatgtt tttttaaatt ctctgtttaa
     2521 actaaagtct ttattcataa ctttagtaat aatatctaca gctattttaa gaatgttcat
     2581 ttatttagcc tttctaaaaa attagttaag taaggctttt ttaaactaac gctaccaata
     2641 tcaccatgct tgtaatcatc aaaacgatga aagtcagatc ccgcagtaac taataaatta
     2701 tgcctcttac ataaagccaa attatctata atttgtttat cagttgcatt aggataaaac
     2761 acttctatgc catcaatccc atattctaag aattcttcta ctttattctt tttgtataaa
     2821 atcgggtgag ctaaaatagc aattccacct gcatctttaa taacctttat tccttccttg
     2881 cttgatattt tcgtacttgg aatataggct gggcagttat caccaataaa attctcaaaa
     2941 atttcttctc tttcatatgg ataaccagct tcaataatta aactagctat gttgtatctt
     3001 gtaatcgtaa ctttaggatc aagtttacct aaatcaattt ctatattaaa gtattctttc
     3061 agtctccttt gaatttcctt tgctcttttt tccctaacct cttgcatatg ttttaagaac
     3121 ttctctattt ttaaaatatc ctctttttta gaaaaatatc ctaaaatatg aatagattca
     3181 ttgttattga ttgtagaaag ctctaaacca taaataccaa ctaggtcaaa tttaggtgct
     3241 aaagctaccg cttcaacagt acctgcaata caatcgtgat ctgttacaga aaaatattta
     3301 atcttttgtt tttgtaactc ttctaatatt tcttcaacac ttaaagaacc atctgagtga
     3361 gtagtatgca tatgaaaatc tccacgcatt gctatctccc acctcctaac tcatagaaaa
     3421 ttaaaataac aaagtatctt ttttctaact tttgatataa ttcactaaat tgtaaatagt
     3481 catataactc tacattagac gtagttttca taataaactt aaacttatta aataaagcta
     3541 tccattcttt aaattcaaaa tttaaaatag catcactttt taaaaagtaa ctataattat
     3601 aaatagtagc ataagactta tttttacctt tttctaagca atttactaag gctttaactg
     3661 ttttcacaat taaatctcca ctataataat aaatatcata gtaagcatgc tctaaattat
     3721 tataattctt aatcaaatca tttacttctc ttgctaaacc ttcaagttcg taagcttttt
     3781 tatctttaat tgcagctaag gatgtcattt taatattacg aaaatacgca tcatataaat
     3841 agttcaaaat tgcatttgct tctttaaatt ctttctcaca ggcacttatt ttagctataa
     3901 tcacatccac ttcttcaaca ttt
//
LOCUS       DAOSGS010000083         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_22407_length_3774_cov_3.226405, whole genome shotgun sequence.
ACCESSION   DAOSGS010000083 DAOSGS010000000
VERSION     DAOSGS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22407_length_3774_cov_3.226405"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PLQ22_03720"
     CDS             <1..1204
                     /locus_tag="PLQ22_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH59101.1"
                     /translation="SPNGIFTKEMDTMDVWFDSGSSHHAVLGPNGLKYPADLYIEGSD
                     QYRGWFNSSLITGVSYYKEAPYKEVISHGFVLAGDEKMSKSLGNVIKPDAVCNKYGAD
                     ILRLWVASVDYTADVSLTDEILVQVSENYRKIRNTFRFLLGNLFDYDNNKNKVKYEDL
                     GETDQYIIVRLNQLIKDVRRAYDEYDFATVYHLVLNYMTNILSSFYLDYSKDVVYVEA
                     ANSLLRRRMQTVFYENLFTLVRLLNPILPHTTEEVYQMMSNTKEKSVYLENMPEAKEY
                     KNSEELEEKYEKFLKLRDDVLKALEVARTNKVIGKSLVSKVVIKPTKEVKELISSLKT
                     DLALVFIVAEFKVDDSLKEGDAYESGLIYVTAKDGYKCDRCWQIKDEVDEDNLCARCA
                     KVIKNKEE"
     gene            1206..2075
                     /locus_tag="PLQ22_03725"
     CDS             1206..2075
                     /locus_tag="PLQ22_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59102.1"
                     /translation="MPINLHFFRDNSLDKLDFARVFDFFDQLGYFEIFYTEDDVQIIA
                     KDEDFRFSYRYLITKKSLVKDIYKLNPKYSNVNFMIELPIMIPSYLAKEIFAIISKLC
                     RSFDLAIYHDSFKDVQEFNIVDLDNLFNSMRQKYIEDYGYGNKRPYDSKKLSEICKYQ
                     RSIEQLATFYEGRKITVPKAQAIEDSITKDFGISCMFRVGEATIFPPHLDYIYVHDEL
                     NNVILVPRADFFSIMGKYLIEIEAVLPDMYILREKQARSSRKVASKLRKFAIDHINYT
                     NLRLCDLIDEQNS"
     gene            2131..2709
                     /locus_tag="PLQ22_03730"
     CDS             2131..2709
                     /locus_tag="PLQ22_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59103.1"
                     /translation="MFKAGYGLCSIDASSLSLKYLFDKLTIGEATIIINVLLVVISEL
                     IKPSLARLLAIPISFAFGYALDGALWTLKWLEFNEITGPIFFTLGGFMTPFGSAFVVT
                     ANLPPLAGDTVVGSLLYRFKGNAGKAKLGAEIIFFVMALVFCILATILTDFKFTQGLS
                     IYTIFLFGIASIFFPKFLSKVQGGYEYVQLVY"
     gene            2684..3361
                     /gene="deoC"
                     /locus_tag="PLQ22_03735"
     CDS             2684..3361
                     /gene="deoC"
                     /locus_tag="PLQ22_03735"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOH59104.1"
                     /translation="MNMYNSYIDHTLLGATAKISDIKKLCDEAKQYSFKAVCVNPCYV
                     RLAKEQLKGSNVLVATVVGFPLGANTFEMKKAEALDAIKNGADEIDMVVNVGMVKDHN
                     WEYVRRDIEKVHNACKMNILFGKKTLKVILETCYLTKEEIEEVCKIASEVGADFVKTS
                     TGFGTGGAKVEDVKLMKESISPSMEVKASGGIRDLATLKAMVEAGATRIGCSSSVKIM
                     EELGEEK"
     gene            3361..>3774
                     /locus_tag="PLQ22_03740"
     CDS             3361..>3774
                     /locus_tag="PLQ22_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase DeoD-type"
                     /protein_id="HOH59105.1"
                     /translation="MAIPTPHIEAKHKDLFGKTVLMPGDPLRAEFIAKTYLEDVTRVN
                     GVRNMFGFTGYYKGKKVSVMGSGMGMPSIGIYSYELFKFYDVEKIIRVGSMGAYSEEL
                     KLYDVVIVSDSYSESSFAKVAYDYDEKVISSSHELT"
BASE COUNT         1296 a          426 c          744 g         1308 t
ORIGIN      
        1 aagtcctaat ggtatcttta ctaaagaaat ggatacaatg gacgtttggt ttgatagtgg
       61 ttcaagtcat catgcagttt taggccctaa tggtcttaaa tatcctgctg atttatatat
      121 tgaaggtagt gaccaatatc gtggttggtt taactcaagt ttaattactg gtgtttcata
      181 ctataaagaa gcaccttata aagaagtaat ctctcatggc tttgttttag ctggtgatga
      241 gaagatgagt aaatctttag gtaatgtaat taagccagat gctgtatgca ataaatatgg
      301 agctgatatt ttaagacttt gggttgcatc agtagattat acagctgatg taagtttaac
      361 tgatgagatt ttagttcaag tgtctgagaa ttatcgtaag attagaaata cctttagatt
      421 tttacttggt aacttatttg attatgataa taataagaat aaagtaaaat atgaagattt
      481 aggggaaact gatcaatata ttattgtaag attaaatcag ttaattaaag atgtaaggcg
      541 tgcttatgat gaatatgact ttgcaactgt ttatcattta gtattaaact atatgactaa
      601 cattttatct tcattttact tagactattc aaaagatgtt gtttacgtag aggcagctaa
      661 tagtttatta agacgccgta tgcaaacagt tttctatgag aatttattta ctttagttag
      721 actacttaat ccaattcttc ctcatacaac tgaagaagta taccaaatga tgtctaatac
      781 aaaagaaaag tccgtttatt tagaaaatat gcctgaggct aaagaatata aaaatagtga
      841 agaattagaa gaaaaatatg aaaagttctt aaaattaaga gatgatgttt taaaggcctt
      901 agaagttgct cgtactaata aagttattgg taaatcttta gtaagtaaag ttgttattaa
      961 gccaactaaa gaagtaaagg aactaattag ttcattaaag actgacttag ctttagtttt
     1021 tatcgtagct gagtttaaag tagatgatag cctaaaagaa ggcgatgcat atgaatcagg
     1081 tttaatttat gtaacagcta aagatggtta taaatgtgac cgttgctggc aaattaaaga
     1141 tgaagttgat gaagacaatc tatgcgctag gtgcgctaaa gttataaaaa acaaggagga
     1201 ataggatgcc tattaattta catttttttc gtgataacag tttagataag ttagactttg
     1261 caagagtatt tgactttttt gaccaacttg gatattttga gatcttttat actgaagatg
     1321 atgtacaaat tatcgctaag gatgaagatt ttcgtttttc ttatcgttat ttaattacta
     1381 agaaaagttt agtaaaagat atttataagt taaatccaaa atattcaaat gtgaacttta
     1441 tgattgaact tccaattatg attccatcat atttagctaa agaaatcttt gcaattattt
     1501 ctaaattgtg tcgtagtttt gatttagcta tttatcatga tagttttaaa gatgtgcaag
     1561 agtttaatat tgtagactta gataatctct ttaatagtat gagacaaaag tatattgaag
     1621 attatggtta tggtaataag agaccttatg attctaagaa actaagtgag atttgcaagt
     1681 atcaaagatc aattgagcaa cttgccacat tctatgaagg aagaaagatt acagttccta
     1741 aggcccaagc aatcgaagat tcaattacta aagattttgg aattagttgt atgtttagag
     1801 taggagaagc tacaatcttc ccgcctcatt tagattacat ctatgtccac gatgagttaa
     1861 acaatgtaat cttagttcca agagctgatt tctttagtat aatgggtaag tatttgattg
     1921 agattgaggc agtacttcct gatatgtata tcttaagaga aaaacaagca agaagcagta
     1981 ggaaggttgc atctaagttg agaaagttcg caattgatca tatcaattat actaacttaa
     2041 gattatgtga tttaatcgat gaacaaaata gttaaaagaa taatatttta tatttttgga
     2101 gtggtagtaa tatcttatgg tgttgcattt atgtttaaag caggatatgg cctttgttca
     2161 attgatgcat catcattaag tttaaagtat ttatttgata aacttacaat tggggaagcg
     2221 acaattatta ttaacgttct tttagttgta atttcagaat tgataaaacc atcacttgca
     2281 agattactgg caataccaat tagttttgct tttggttatg ctttagatgg ggctttatgg
     2341 acgcttaagt ggctagagtt taatgagatt actggtccta ttttctttac tttaggtgga
     2401 tttatgacac cttttggtag tgcgttcgta gttacagcta atttaccgcc actagcaggt
     2461 gatactgtag tagggtcact tttatataga tttaaaggta atgcaggtaa agcaaaacta
     2521 ggagcagaaa ttatcttttt tgtgatggct ttagttttct gtattctagc tacaatctta
     2581 acggatttta agtttactca aggattgagt atctatacaa tctttttgtt tggaattgct
     2641 agtatctttt tccctaagtt tttaagtaaa gtacaaggag gttatgaata tgtacaactc
     2701 gtatattgat cacactttat taggtgcaac agcgaaaata agtgatatca agaaattatg
     2761 tgatgaggct aaacagtata gttttaaggc tgtttgtgtt aatccttgtt atgtaagatt
     2821 agctaaagaa caattaaaag gctctaacgt tttagttgca actgttgttg gttttccttt
     2881 aggagctaat acttttgaaa tgaaaaaagc agaagcttta gatgctatta aaaatggggc
     2941 tgatgaaatt gatatggtag ttaatgttgg gatggttaaa gaccataact gggaatatgt
     3001 tagacgtgat attgaaaaag tgcacaatgc ttgtaagatg aacatccttt ttggtaaaaa
     3061 aactttaaaa gttattttag agacttgtta cttaactaaa gaagaaattg aagaagtttg
     3121 taagatagca agcgaagttg gggcagattt tgtgaagaca tcaactggct ttggcactgg
     3181 tggtgctaaa gtagaagatg ttaagttaat gaaagagtca attagtcctt ctatggaagt
     3241 aaaagcaagt ggtgggatta gagatcttgc tactttaaaa gcaatggttg aagctggtgc
     3301 tacaagaatt ggttgttcta gtagtgtaaa aattatggaa gaattagggg aggaaaaata
     3361 atggctatac ctacaccgca tattgaagca aaacataaag atttatttgg aaaaactgtt
     3421 ttaatgccag gagatccttt aagagcagaa tttattgcca agacatattt agaagacgta
     3481 acaagagtta atggcgttcg taatatgttt ggttttacag gctattataa aggtaaaaag
     3541 gtttcagtaa tgggatcagg aatgggaatg cctagtattg gtatttactc atatgaatta
     3601 tttaagtttt atgatgtaga gaaaattatt agagttggtt caatgggagc atattcagaa
     3661 gaattaaaac tatatgatgt agttatcgta tctgattcat actcagagtc tagttttgcg
     3721 aaggtagcat atgattatga tgaaaaagtt atttcatcaa gccatgaact aact
//
LOCUS       DAOSGS010000084         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_22696_length_3740_cov_4.213840, whole genome shotgun sequence.
ACCESSION   DAOSGS010000084 DAOSGS010000000
VERSION     DAOSGS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22696_length_3740_cov_4.213840"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLQ22_03745"
     CDS             <1..520
                     /locus_tag="PLQ22_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH59106.1"
                     /translation="SAMSYDLALNAKHQEEIFLVPAFTGLGAPYWDNEVRGAIFGITK
                     DTSREDITSATLDAIAYQVKDVIGLMEKEAKIQLHNLKVDGGACENDYLMQFQSNILP
                     ATIYRPKIIESTALGVAYMAGLASGYFKSIEEITKLHQIERVFTNKMNEKKSKELYQK
                     WLTAVKAARMFK"
     gene            542..2218
                     /locus_tag="PLQ22_03750"
     CDS             542..2218
                     /locus_tag="PLQ22_03750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH59107.1"
                     /translation="MANQIKFFALGGLGEHGKNMYIVEVNDRIFILDSGIKNPGLDLL
                     GVDAVLPDMEYLLENKDKVKALFLSHGHIENIGAVPEILKEMNIGVYGTHFTISILEK
                     ELTELGMNIKDYRLYRINEDKELMFGNVKVSFYATSHSIPESLGIVISTPDGAIVYAP
                     DFTFSANSDKKYKTGFHRLGEISKEKVLLLLPESLGSSNIGRTVSDNILIHTVTEQLK
                     TKGRTIFAMYSNDLDRMQKIINIASSSNKRIAIIGKRTRKIISVAMESNYLNIPSDKF
                     VNLKYIDDSNDNDYEDLVVIVTGELNEPFIHVQRMASGKDRFIKIKDTDTIVLISPHV
                     KGYPNTFANTMNALARTNAKVINMTKKDLRSSHASPEDLKMLYMMLNPEYIAPVIGEY
                     RHQYAHMQVALDAGYSREEILLLENGEVITFLDGKLQVAKEFVPTGDVLVDGSIVGDI
                     NSVVLKDREMLAQGGVVIINVIVDARYKEVLAEPSIVLKGVTNNKQVEADIYDIINEV
                     VDDTLDYHFDEKYLDWQVRKKEIKDVVSKRIMKECGLSPIVLAVVLDEEK"
     gene            2218..>3740
                     /gene="alaS"
                     /locus_tag="PLQ22_03755"
     CDS             2218..>3740
                     /gene="alaS"
                     /locus_tag="PLQ22_03755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOH59108.1"
                     /translation="MKKLSSLEIRKLWLEFWEEKGHLIIPSASLIPHDDPTLLWINAG
                     IAPLKKYFDGRETPKRNRLANVQKSIRTNDIENVGVTARHHTFFEMLGNFSIGDYFRK
                     EALTWAYEILFSEKWFALDKKNIYFSYYPEDLETKKMWMKLGVEEERLIPLERNFWEI
                     GDGPCGPCTEIFYDRGLKYDPENIGLRLIIDDLDNDRYVEIWNIVFSQFNAESGKKRE
                     EYKELPSKNIDTGMGFERIVSILQETDTNFETDLFMPIIKDIEKLSGVKYDGQMAFKV
                     IADHVRSVTFAISDGANVSNEGRGYVLRRLIRRAVRYGKKLGLNKPFLASLVDRVVSI
                     MKEPYPNLVKAKELVKVVVNNEEEKFLKTLDKGEKKLLDYMEQAKGKVITGEIAFLLY
                     DTFGFPIELTLEVGKEHGFEVDLKAFEEELEKQRKRARASRDEDESMISQNEDMLKFK
                     EESIFTGYSELKRVSKVIAIFKDGTSFKEANGEVLLVFDQTPFYGLKGGQVGDQGKL"
BASE COUNT         1369 a          391 c          761 g         1219 t
ORIGIN      
        1 atcggctatg tcttatgatt tggcgttaaa tgctaagcat caagaagaaa ttttcttagt
       61 accagctttt actggcttag gtgcaccata ttgggataat gaagttagag gtgctatctt
      121 tggtattaca aaagatactt ctagagaaga tataactagt gctactttag atgctattgc
      181 ttatcaagta aaagatgtaa ttggcttaat ggaaaaagaa gctaaaattc aattgcataa
      241 tttaaaggtt gatggtggag catgtgagaa tgactattta atgcaatttc agtctaatat
      301 cttaccggct acgatttata gaccaaagat tattgaatca acagctttag gtgttgctta
      361 tatggcaggt ttagctagtg gctattttaa aagtattgaa gagataacaa agttacacca
      421 gattgaacgt gtctttacaa acaaaatgaa tgaaaagaaa agtaaagagt tataccaaaa
      481 gtggttaaca gctgttaagg ctgcacgaat gtttaaatag ttaaaaaata cgaggtgatt
      541 tatggcaaat caaattaagt tttttgcttt gggaggctta ggtgaacatg gcaaaaacat
      601 gtatattgta gaggtaaatg atagaatctt tattttagat tctggaatta agaacccagg
      661 tttagatttg cttggagtcg atgcagttct tccagatatg gaatatcttt tggaaaataa
      721 agataaagta aaagctttgt ttttatctca tgggcatatt gaaaatattg gtgctgttcc
      781 tgagatttta aaagagatga atatcggtgt ttatggaact cactttacaa tttctatatt
      841 agaaaaagaa ttaactgaat taggaatgaa tattaaagac tatcgtttgt atagaataaa
      901 tgaagataaa gagttaatgt ttggtaatgt taaggtatct ttctatgcca caagtcatag
      961 tattcccgaa agtttaggta tcgtaattag tactcctgat ggagcaatag tttatgctcc
     1021 tgactttact ttctctgcta actcagataa aaaatataaa acaggatttc atcgtttagg
     1081 tgagatttca aaagaaaaag tattactact tttacctgaa agtttaggtt caagtaatat
     1141 tggtaggact gtaagtgata atattttaat tcatacagtt actgagcaat taaaaactaa
     1201 aggtagaaca atctttgcga tgtattctaa tgacttagat agaatgcaaa agattattaa
     1261 tattgccagt tcctcaaata aaagaatagc tattattggt aaaagaacaa gaaaaattat
     1321 tagtgttgcg atggaatcaa attatttaaa tattccaagt gataaatttg taaacttaaa
     1381 gtatattgat gatagcaatg ataacgatta tgaggactta gtagttattg taactggtga
     1441 attaaatgaa ccatttattc atgttcaaag aatggctagt ggtaaagata gatttattaa
     1501 aattaaggat acagatacta tagttttaat ttcaccgcat gtaaagggtt atcctaatac
     1561 ttttgcgaat acaatgaatg ctttagcgag aactaatgcg aaagttatta atatgacgaa
     1621 gaaagattta cgtagttctc atgcttcacc agaagactta aagatgcttt atatgatgtt
     1681 aaacccagaa tatatcgcac cagttattgg tgaatatcgt catcaatatg ctcatatgca
     1741 agttgcttta gatgctggtt atagtagaga agagatttta ctacttgaaa atggtgaagt
     1801 aattaccttt ttagatggta agttgcaagt cgcaaaagag tttgtaccaa caggtgatgt
     1861 tttagttgat gggtcgattg ttggggatat taatagtgtt gtattgaaag acagggaaat
     1921 gttagctcaa ggcggagtag ttattattaa tgtaattgta gatgcaagat acaaagaagt
     1981 attagctgaa ccttcaatag ttttaaaggg agttacaaat aataaacaag tagaagctga
     2041 tatttatgat ataataaatg aagtagttga tgatacttta gactatcatt ttgatgaaaa
     2101 gtatttagac tggcaagtaa gaaagaaaga aataaaagac gttgtaagta aaaggattat
     2161 gaaagaatgt ggtttaagtc caattgtact tgcagtagtc ttagatgagg aaaaataatg
     2221 aaaaaactaa gtagtttaga aattagaaaa ttgtggcttg aattttggga agaaaaggga
     2281 catttaatta ttcctagtgc ttctttaatt cctcatgatg atccaacgtt actttggatt
     2341 aatgcgggga tagctccttt aaagaaatat tttgatggaa gagaaactcc aaaaagaaat
     2401 aggttagcta atgtgcaaaa atcaattaga actaatgata ttgaaaatgt aggggtaact
     2461 gcaagacatc atacgttttt tgaaatgtta ggaaactttt caattggaga ttattttagg
     2521 aaagaagcat taacatgggc ttatgaaatt ctattttctg aaaaatggtt tgctttagat
     2581 aaaaagaata tttacttttc atactatcca gaagatttgg aaacgaagaa aatgtggatg
     2641 aagctaggag tagaagaaga aaggttaatt cctttagaga gaaacttttg ggaaattggt
     2701 gatggtcctt gtggtccgtg tactgagatc ttttatgacc gtgggttaaa atatgatcca
     2761 gaaaacatag gtttaagatt aatcattgat gatttagata atgatcgtta tgtagagatt
     2821 tggaatatcg tttttagtca gtttaatgct gagtcaggta aaaaaagaga agagtataaa
     2881 gaactaccaa gtaagaatat tgatactggt atgggctttg aaagaattgt ttcaatctta
     2941 caagaaactg atactaattt tgaaactgat ctttttatgc ctattattaa agatattgag
     3001 aaattaagcg gtgttaagta tgatggtcaa atggcattta aagttattgc agaccatgtt
     3061 agaagtgtta cttttgcaat ttcagatggt gctaatgtaa gtaatgaagg tcgtggttat
     3121 gttttaagaa gactaattag aagagctgtt cgttatggta aaaagttagg attaaataaa
     3181 cctttcttag ctagtttagt tgatagagtt gtaagtatta tgaaagagcc ttatcctaat
     3241 ttagtaaaag ctaaagagtt agtaaaagtt gtagttaata atgaagaaga aaaattctta
     3301 aagactttag ataaaggtga aaagaaactt cttgattata tggagcaagc taaaggtaaa
     3361 gtaattacag gagagattgc atttctgtta tacgatactt ttggatttcc aattgaacta
     3421 actttagaag ttggtaaaga acacggtttt gaagttgact taaaagcttt tgaagaggaa
     3481 cttgaaaaac aaagaaaaag agctcgtgct agtcgtgatg aagatgagtc aatgattagt
     3541 caaaatgaag acatgcttaa gtttaaagag gaatcaatct ttactggcta tagtgaacta
     3601 aaaagagtaa gtaaagtaat tgcaatcttt aaagatggaa cttcatttaa agaagctaat
     3661 ggtgaagtat tattagtctt tgatcaaact ccattttatg gtctaaaagg tggtcaagta
     3721 ggagatcaag gtaaattaat
//
LOCUS       DAOSGS010000085         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_23008_length_3702_cov_3.414587, whole genome shotgun sequence.
ACCESSION   DAOSGS010000085 DAOSGS010000000
VERSION     DAOSGS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23008_length_3702_cov_3.414587"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLQ22_03760"
     CDS             <1..619
                     /locus_tag="PLQ22_03760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH59109.1"
                     /translation="ASLIKDSKKIADIGTDHGYLVIEALLEGKTLKAQAIDNKKMPLL
                     RAKENIANFGLEEKVSFSLSNGLDDLEKDVDYIIMSGLGGSLIISLLEENLNKINKQT
                     LILQANRNCAELRSFLSENSFKIEYEEVIFDDKYYEIIVTKKIDEKIKLTDKEVLFGP
                     YNLKHPNNVFYNYLEDEYARYEKIKYPSKQVLHKKSLIKDILAKK"
     gene            complement(679..846)
                     /locus_tag="PLQ22_03765"
     CDS             complement(679..846)
                     /locus_tag="PLQ22_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH59110.1"
                     /translation="MPKFITDACIACGSCQAECPVDCIIEGDIYVIDADLCIDCGACV
                     DVCPTGAIEDK"
     gene            971..1189
                     /locus_tag="PLQ22_03770"
     CDS             971..1189
                     /locus_tag="PLQ22_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOH59111.1"
                     /translation="MIVWWGVLLIGIGALLVGAIVGFFVSRHLSKKHLKENPPVTENM
                     IRAMFKSMGRTPSEKQVRQVMASMEQNK"
     gene            complement(1211..1861)
                     /gene="plsY"
                     /locus_tag="PLQ22_03775"
     CDS             complement(1211..1861)
                     /gene="plsY"
                     /locus_tag="PLQ22_03775"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOH59112.1"
                     /translation="MNIYLEILLLVLSYILGSIPFGLLLGKMKGIDLREVGSKNIGTT
                     NAWRNLGPGLGILTLILDVFKAVIVLGLVRYNVIPNESIVIDPIFYGLAAAIGHTHSI
                     FLKFKGGKAVACTAGVLLVYSPATTIIFAFVFFVTLFISKYVSLGSMMAILVTFLVSF
                     MIPYLGGKDFITDLQLDFDFVIALGIFCVYIFLNHISNIKRIQSGEERKIHLIKKR"
     gene            2068..>3702
                     /locus_tag="PLQ22_03780"
     CDS             2068..>3702
                     /locus_tag="PLQ22_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOH59113.1"
                     /translation="MAKQSYTEKDIQVLEGLEAVRKRPGMYIGSTDSRGLHHLLWEIV
                     DNSIDEALAGEANFIQVTIHKDNSATVTDNGRGIPIGRHKSGRPTPEVVFCTLHAGGK
                     FDASGGYKVSGGLHGVGSAVVNALSSIVEVRIRRDGKEFYQKYEKGGSVIRSPKVINY
                     SGHRTGTEITFKPDPAIFSTTRFDNNIIKERLKESAFLIKGLKIKLDDERHGTSDTFQ
                     FKNGIIEFLESKTKGRKSFHDALFFEGTREQISIEVAMQFGSEAYGEDIVSFVNNINT
                     KDGGTHETGFRAGLTRAFNDYARMKEFLKDRDPNLEGSDIREGLTAIINIRIPETILQ
                     FEGQTKNKLGTPEAKSALESFVNERVGFFLAEKGEIANKLIDKMIRAANAREAARKAR
                     ADARKVKKIVSKKTNLTGKLVPTQKKNPDKNELFIVEGDSAGGSAKQGRDSVFQAILP
                     LRGKVLNAERTKPEDLLKNEEILSLVHSIGAGFGQDFNISKSNYKKIIIMTDADDDGA
                     HIRILLLTFFFRYMTELIENGYIYIAQPPLYKVTTRK"
BASE COUNT         1386 a          472 c          666 g         1178 t
ORIGIN      
        1 tgcatcttta attaaagatt ctaaaaagat agctgatatt ggtactgacc atggttattt
       61 agtaattgaa gctttacttg aaggaaaaac actcaaagct caagcaattg ataataaaaa
      121 aatgcccctt ttaagggcta aagaaaatat tgcaaatttt ggtttagaag aaaaggtaag
      181 tttttctcta tctaatggct tagatgattt agaaaaagat gtggattata ttattatgag
      241 tggtttaggt ggaagtttaa ttataagttt acttgaagaa aatttaaata agataaacaa
      301 gcaaacttta atcttgcaag caaatcgtaa ctgtgcagag cttagatcat ttttatcaga
      361 aaactcattt aaaatagagt atgaagaagt tatttttgat gataagtatt atgagattat
      421 agtaactaaa aagattgatg aaaaaattaa acttactgat aaagaggttc tttttggacc
      481 ttacaatcta aaacatccaa acaatgtctt ttacaactat ttggaagatg aatatgctag
      541 atatgagaag attaaatatc ctagcaaaca agtactgcat aaaaagtctc taataaaaga
      601 tattttagct aaaaagtaat gtatttatta gtatatcgat atcttgtaat aaaaaaagtg
      661 gtcaaaccac tttttttctt atttgtcttc gattgcacct gtagggcaaa cgtcaacgca
      721 tgcgccacaa tcgatacata aatctgcatc aattacgtaa atatcgcctt caataatgca
      781 atcaactgga cattctgctt ggcatgagcc acaagcaata caagcatctg taataaattt
      841 tggcatacta atcctccttg tacgttgata attataacat ttattttaga aagtatcaag
      901 tttaatttac aaaaaaattg atttttatta gggcttttgg tataattaaa ttatagaaga
      961 ggtgataatt atgatcgttt ggtggggagt attattaatt ggtattggtg ctttattagt
     1021 aggtgcaata gttggttttt ttgtatctag acacttatct aaaaaacatc taaaagaaaa
     1081 tcctccagtt actgagaaca tgattagggc gatgtttaaa tcaatgggta gaacaccatc
     1141 tgaaaaacaa gttcgccaag ttatggctag tatggaacaa aacaaataga ataaaaaaag
     1201 atgatttaaa tcatcttttt tttattaagt gtatttttct ttcttcgcca ctttgaattc
     1261 ttttaatgtt tgagatatga tttaaaaaga tgtaaacaca aaagattcct aatgcaatca
     1321 caaagtcaaa gtctagttgt aagtcagtaa taaagtcttt accacctaaa tatggaatca
     1381 taaaagaaac taaaaaggta actaaaatag ccatcattga acctaaagaa acatatttag
     1441 aaataaataa tgttacaaaa aagacaaaag caaagataat agtagtagct ggagagtaaa
     1501 caagtaatac acctgctgta catgcgacag ctttaccgcc tttaaacttt aagaagattg
     1561 aatgagtatg gccgattgca gcagctaagc cataaaagat tggatcgata acaatacttt
     1621 cattgggaat cacgttatac cttactaaac ctaaaacaat aacagcttta aagacatcca
     1681 agattaaagt aagaattcct aatcctggtc ctaggttgcg ccaggcattg gtagttccga
     1741 tattttttga gccaacttcc cttaagtcaa ttcctttcat tttaccaagt aaaaggccaa
     1801 agggaattga tcctaaaata tatgataaga ctaataataa tatttctaga tagatattca
     1861 taaaatcacc ttaattatta taccatattg tttatatttt tatactaaaa atataaaaaa
     1921 agtattttaa ttttcattta tttaagtatt tttatataaa atagttataa aacatattca
     1981 attgtcaaaa atgaatatga taaaatataa aaataagtgt ttacttttag tttaatatgt
     2041 ggtataatat ataaaaggag tggttttatg gcaaaacaaa gttacacaga aaaagatatc
     2101 caggttttgg aaggattaga ggccgttaga aaaaggcccg ggatgtatat tggctcaact
     2161 gattcaagag gtttacatca tttactatgg gagatagtag ataactctat tgatgaggcc
     2221 ttagcaggag aagctaattt tatccaagtt acaattcaca aagataattc ggctactgtt
     2281 actgataatg gtagaggtat tccgataggc agacataagt caggtagacc aactcctgag
     2341 gttgtttttt gtacacttca tgctggtggt aaatttgatg catctggagg ttataaagtt
     2401 tcaggtggtt tacatggtgt tggttcagca gtagttaatg ctttaagttc gattgttgaa
     2461 gtgagaatta gacgtgatgg caaagaattc taccaaaagt atgaaaaagg tggatcagta
     2521 atcagaagcc cgaaagttat aaattatagt gggcatcgta ctggtacaga aattaccttt
     2581 aaaccagacc cagctatctt tagtacaact agatttgata ataatattat taaggaacga
     2641 ttgaaagaat cagctttttt aattaaaggt ttaaaaatta aattagatga tgagcgtcat
     2701 ggtactagtg atacttttca atttaaaaat ggtattattg agtttttaga atcaaagact
     2761 aaaggacgta agagtttcca tgatgcattg ttttttgaag gaacaagaga gcaaattagt
     2821 atagaagttg caatgcaatt tggatctgaa gcttatggtg aagatattgt atcgtttgta
     2881 aataacatta atacaaaaga tggtggaact catgaaactg gttttagagc cggcttaaca
     2941 agagcattta atgattatgc gagaatgaaa gagttcttaa aagatcgtga tcctaattta
     3001 gagggttcag atattcgtga aggattaaca gcgattatca atattagaat acctgaaaca
     3061 attttacagt ttgaaggaca aactaaaaac aaactaggaa ctccagaagc taagagtgca
     3121 ctagagagtt ttgtgaatga aagagttggt ttcttcttag ctgaaaaagg tgaaattgct
     3181 aataagctaa ttgataagat gattagggca gctaatgcga gagaagctgc cagaaaagca
     3241 agagctgatg ctagaaaagt aaagaaaatt gtttcaaaga aaactaatct tacaggtaag
     3301 ttagttccta ctcagaagaa aaaccctgat aaaaatgaat tgtttattgt agagggagat
     3361 tctgcaggtg gttccgcaaa gcaaggcaga gatagtgtgt ttcaagctat tctaccttta
     3421 agaggtaaag tattgaacgc tgaaaggaca aagccagaag atttacttaa aaatgaagag
     3481 attttatcat tggttcattc tataggtgct ggttttggcc aagattttaa tattagtaaa
     3541 tcaaattata aaaagattat tattatgact gacgctgatg atgacggagc tcatattcga
     3601 attcttttac taacattttt ctttagatat atgactgagt taattgaaaa tggttatatt
     3661 tatattgctc agccaccttt atataaagtc actacaagaa aa
//
LOCUS       DAOSGS010000086         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_23331_length_3666_cov_3.678205, whole genome shotgun sequence.
ACCESSION   DAOSGS010000086 DAOSGS010000000
VERSION     DAOSGS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23331_length_3666_cov_3.678205"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLQ22_03785"
     CDS             complement(<1..1014)
                     /locus_tag="PLQ22_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59114.1"
                     /translation="MKKILSAFLFLLCILGLKGMQAKAIDWEFIPQGINYISKNQFIL
                     TETDDDVILTSYRYIKVDGSIMYWLRAGSENNLNGITCEYNIYNSNKDLVDEIGWFSL
                     NKSGVPVDLSVSNKYLRISFKYPKVSNTDLTPEQVFDNLEIYFNLYDPNLFNPDSEFD
                     YKGVDYSSYSFINSKDVRISYQDSFSLTDFESNLLAYDNYDGNITSKITKDVDTYSSK
                     ENIVGNYLVQYSVQDEELNESTFTLNLEIYDEIPPIITGSSNYTKNVGDELSVSELKT
                     VLGLEAYDDYDGNITNNLFIQEDYYTGNENVVGEHIVIYQVADSSNNTASFTATIKVN
                     DNEG"
     gene            complement(1098..1886)
                     /locus_tag="PLQ22_03790"
     CDS             complement(1098..1886)
                     /locus_tag="PLQ22_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012669946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH59115.1"
                     /translation="MLEIKNVTKIFNPDIIEDKRVALDSLSLTIEEGEFVTVIGSNGS
                     GKSTLLNIINGKHLPDVGKVILDGEDITNLKQHQKAKYIGTVFQDPHQGTASQMSVLE
                     NLEIAKRRGKRKTLRWGFSAQNREDFIIQLSKLDLGLENRLTQRIGTLSGGQRQAITL
                     IMATLNNPKLLLLDEHTAALDPNTATKVLEITNDIIKAEKLTTIMVTHNMRDAINYGD
                     RLIMLDQGKIIFDCKGEEKKNLTIPILLQKFSSKSVINDELILS"
     gene            complement(1880..2815)
                     /locus_tag="PLQ22_03795"
     CDS             complement(1880..2815)
                     /locus_tag="PLQ22_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH59116.1"
                     /translation="MELVSILFDSLQEGLAYAILVVGVFISFRMLDFADMTCEGSLTM
                     GAAITMVMITSGVNPLLSVVVAFVLGTVAGIITALLHTKLKIPPLLSGIIMMTALYSI
                     NLQVIQGGKSTIIMTYGETKTVFSPLEKLIPTTSFITNFQSSKLIVLALFLILVFFAI
                     YYFFGTEVGMSIRSTGMNKDMSKSLGINTDLMIIIGLALSNGLIALSGSLIAQKNGSS
                     NMDIGRGTLVIGLAAIILGEAIFGKRTFKNWLISVILGSFIYNIIVAIAINLFGLNPN
                     FLKLIQAVLIVIILALPLIKSQIKKFKTRKELNAC"
     gene            complement(2830..>3666)
                     /locus_tag="PLQ22_03800"
     CDS             complement(2830..>3666)
                     /locus_tag="PLQ22_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH59117.1"
                     /translation="FLEVLEENGFKDGENIKVTLEIPNADQTVMMQQAKSLVRNSDLI
                     LSIATNATAAAQNALVEANKTTPLLFTAVTDPVAHGFVESMAKPGGHITGTSDMNPVA
                     EQVGLAKELLPEATKIGFIYNSNEENSRIQVEMGVAAAEALGMTTVVKTISEVNQIDL
                     VANNLFNEVDVVYVPTDNMLAQNMVLLTNMSLSKKKPIIAGEENQVRYGASITFGINY
                     RELGRQTGQMAVEILKNGKLPKDIPAATSENLSLIVNKTNLEAMGITVSQALLDRADE
                     VI"
BASE COUNT         1223 a          646 c          443 g         1354 t
ORIGIN      
        1 accttcatta tcattcactt taatggtagc agtaaatgaa gcagtattat ttgaactatc
       61 agctacttga taaataacaa tatgttcacc aacaacattt tcgtttcctg tataataatc
      121 ttcttgaata aaaagattat tagtaatatt gccatcataa tcatcataag cttctaaacc
      181 taaaactgtt tttaattcac taacacttag ttcatcgcca acatttttag tataattact
      241 tgaacctgta ataattgggg gaatttcatc ataaatctct aaatttaaag taaaagtcga
      301 ttcatttaac tcttcatctt gaactgaata ttgtactaaa taattcccaa caatattctc
      361 tttactacta taagtatcta catcttttgt gatttttgat gttatattcc catcatagtt
      421 atcataggca agtaagttgc tttcaaaatc agttaaacta aaactatctt gatatgatat
      481 ccttacatct ttgctattta taaagctata cgaactataa tctacacctt tataatcaaa
      541 ttcactatca ggattaaaaa gatttggatc atataaatta aaataaattt ctaagttgtc
      601 aaaaacttgt tctggtgtta aatcggtatt gctaacttta ggatatttaa aacttattct
      661 taaatattta ttgctaactg ataaatctac tggtacgccg cttttattta aagaaaacca
      721 gccaatttcg tcaactaaat ctttattgct attataaata ttatactcac aagtaatacc
      781 atttaaattg ttttctgacc ctgcccttaa ccaatacata atactaccat ctactttaat
      841 gtagcgatat gatgtcaaga taacatcatc gtctgtttct gttagaataa attgattttt
      901 agaaatataa tttattcctt gaggaataaa ctcccaatca attgccttag cttgcattcc
      961 ttttaaacca agtatgcata aaaggaataa aaatgcactt aatatttttt tcattatacc
     1021 tcctctttac ttgtatatta ttaagaatca gtataatttc ttttatttaa ttaaaaaagg
     1081 tcacttaaag tgacctttta acttaagatt agctcatcat taattacaga tttagaagag
     1141 aatttttgaa gaagaattgg aattgttaga tttttctttt cttctccctt acaatcaaaa
     1201 attatctttc cttgatcaag cattattagc ctatcgccat aattaattgc atctctcata
     1261 ttatgagtta ccataatcgt cgttaatttt tctgctttaa taatatcatt agtaatttct
     1321 aatacttttg tagctgtatt agggtctaaa gcagctgtat gttcatctag taataatagt
     1381 tttggattat ttaaagttgc cataattaaa gtaatagctt gcctttggcc accagataga
     1441 gtaccaattc tttgcgttag tctgttttcc aaacctaagt caagtttact taattgtata
     1501 ataaaatctt ctctattttg agcactaaaa ccccatctta aagtctttct ttttcctcta
     1561 cgcttagcaa tttctaagtt ctctaaaaca gacatttgac tagcagtacc ttgatgtggg
     1621 tcttgaaaga cagtgccgat atattttgct ttttggtgtt gttttaaatt tgtaatatct
     1681 tcaccatcca aaataacctt cccaacatca ggtaagtgct taccattaat aatattaagt
     1741 aaagttgatt tgcctgaacc attactacca ataacagtta caaattctcc ttcttcaatt
     1801 gttaatgata aactatctaa agctactctt ttatcttcta ttatatcagg attaaatatt
     1861 tttgttacat tcttaatttc taacatgcat ttaattcctt tcttgtttta aatttcttaa
     1921 tttgcgattt tattaatggt aaagctaaaa taattacaat taaaacagct tggattaatt
     1981 tcaagaagtt tggatttaag ccaaataaat tgattgcaat cgctacaata atattataaa
     2041 taaatgatcc taaaattacc gaaattaacc agtttttaaa tgttctctta ccaaaaatag
     2101 cttcacctaa aataatcgct gctaacccta ttactaatgt tccacggccg atatccatat
     2161 tactactacc gtttttttga gctattaaag atccacttaa agcaattagt ccatttgata
     2221 gtgctaaacc aatgataatc attaaatcag tatttatacc aagtgactta ctcatatctt
     2281 tgttcattcc tgtacttcta atactcatac caacttctgt accaaagaaa tagtaaattg
     2341 caaagaatac aagtattaag aataatgcta aaactattaa tttagaactt tgaaaattag
     2401 taataaatga agttgtagga attaactttt caaggggact aaaaacagtt tttgtttccc
     2461 cataagtcat aatgatagtc gattttccac cttgaataac ttgtaagttg atggaatata
     2521 aagcagtcat cataatgata ccagataaaa gtggtggtat ttttaatttc gtatgaagta
     2581 aggcagttat tataccagca acagttccta aaacaaaggc aactacaaca cttaaaagcg
     2641 gatttactcc actagtaatc atcaccatcg taattgcagc acccattgtt aatgaacctt
     2701 cgcatgtcat atctgcgaaa tctaacatcc taaaggaaat aaaaacacct acgactaaaa
     2761 tagcatatgc taatccttct tgtaaggaat caaataagat acttactaat tccataactt
     2821 aactcctttc taaataactt cgtcagctct atctaataat gcttggctaa cagtaatacc
     2881 cattgcttct aaatttgttt tgtttacaat tagacttaaa ttttctgatg tagcagcagg
     2941 aatatcttta ggtaattttc cattctttaa aatctctact gccatttgtc cagtttgtct
     3001 tcctaactca cgatagttaa taccaaatgt aattgatgca ccatatctaa cttggttttc
     3061 ttcaccagca ataatcggtt tctttttact taatgacata ttagttaata ataccatatt
     3121 ttgcgctaac atattatcag ttggtacata aacaacatct acttcgttga ataagttatt
     3181 tgcaactaaa tcaatttggt ttacttcact aatagtctta acaaccgtag tcatacctaa
     3241 tgcttcagca gctgcaacac ccatttctac ttgaattctt gagttttctt catttgaatt
     3301 gtagataaaa ccaatcttag ttgcctcagg taataactct tttgcaagtc ctacttgttc
     3361 agctactgga ttcatatcac tagtaccagt aatatgtcca cctggtttag ccattgactc
     3421 tacaaaacca tgagctacgg gatctgtaac tgctgtaaat aaaagcggtg ttgtcttgtt
     3481 tgcttcaact aaagcatttt gcgctgctgc agttgcgttt gtggcaatag ataaaataag
     3541 atctgaattt cttactaagc tctttgcttg ttgcatcata actgtttgat ctgcattagg
     3601 aatttcaaga gtaactttaa tgttctctcc atccttaaag ccattttctt ctaatacttc
     3661 taaaaa
//
LOCUS       DAOSGS010000087         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_23693_length_3626_cov_4.545785, whole genome shotgun sequence.
ACCESSION   DAOSGS010000087 DAOSGS010000000
VERSION     DAOSGS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23693_length_3626_cov_4.545785"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..255)
                     /locus_tag="PLQ22_03805"
     CDS             complement(133..255)
                     /locus_tag="PLQ22_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59118.1"
                     /translation="MYCRNCGCKVAMNDLYCKVCGEGIKSNLEIISKINEKNDQ"
     gene            complement(382..2544)
                     /gene="pnp"
                     /locus_tag="PLQ22_03810"
     CDS             complement(382..2544)
                     /gene="pnp"
                     /locus_tag="PLQ22_03810"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH59119.1"
                     /translation="MKKTFEFKQGSRMIVVETGEMAKQANGAVVTHFGDTTVLTAVVH
                     NSEGFNAGFFPLTVVYQEKLYAAGKIPGGFLRREGRASEVETLNARLIDRPIRPLFPE
                     GFKDEVQIINIVLSSEPDNSPAMTAMLGSSLALMISNIPFNGPIAGVEVGLVDGKFII
                     NPTEEEQAKSELALSIAGTKDAINMVEASSKNISEDVMLEALLFGHEEIKRLVKFQEE
                     VVKEVGLKKAEVFLDLLGEESVAKIEKFIGKELRKAVSIVEKQERAAKIDELTKQTVD
                     YFVSLADTVEAKEEAEKVAKEACENIVSGEVRRLITKEKIRPDGRKTDEIRPLSSRVH
                     VVARPHGSALFTRGQTQAFTTVTLGSLAESQIIDGLTSEESKRFMLQYNFPQFSVGET
                     GRYGGPGRREIGHGALGEKALMNIIPDEDTFPYTIRVVSEIFESNGSSSQASICAGTM
                     ALMDAGVPIKAPVAGIAMGLIMDGKDYTILTDIQGMEDHFGDMDFKVAGTKDGITALQ
                     MDIKISGVTTEILKEALAQAKKGRLEILKHMLGTISEPRKELSPYAPKVKMIRINPDK
                     IKDVIGSGGKVINEIIEKTNNVKIDIEQDGRVFVMHQEYEWIDKAIEIIENIVREAEV
                     GQIYEGTVIRIESYGAFLRLWEGCEGLLHISKIAHEKVAKVEDVLAIGDQIAVKVIGI
                     DEKGRIDLSRKDTIENPNKGKEEVKKDRPPRRFPKKDK"
     gene            complement(2602..2874)
                     /gene="rpsO"
                     /locus_tag="PLQ22_03815"
     CDS             complement(2602..2874)
                     /gene="rpsO"
                     /locus_tag="PLQ22_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOH59120.1"
                     /translation="MSCISKEEKAKLVKEFGKDENDTGSVEVQIAILTAEINRLNEHF
                     AIHPKDHHSNRGLLKKVGHRRNLLDYLKRTNLNSYREVLVKLNLRK"
     gene            complement(2989..>3626)
                     /gene="mtnN"
                     /locus_tag="PLQ22_03820"
     CDS             complement(2989..>3626)
                     /gene="mtnN"
                     /locus_tag="PLQ22_03820"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOH59121.1"
                     /translation="NSWTEEVYHFEFHYGDIDNKKVVLVKSGVGKTNAGLLIGTLLSQ
                     VEVDYIINLGVCGGVSDKVNLFDLAVVDKYFYGDVELPKHEYFYGQMSKCPRYFELNS
                     KLKKTIKDNFPKENYKFVSCYTSDTFVDKDNYPKVKAIIDHHFSHYDISIFDMESTAF
                     AHASYLLDIPFVAIRTISDLVPETNWKEYNLNLEQAIIKSSEFVYKLVLSL"
BASE COUNT         1168 a          711 c          403 g         1344 t
ORIGIN      
        1 aaatgatagt tattaaaaat attattgaac agttttgaaa catcccaaac aattcgtcta
       61 aaacacttcc tataattaca aaatcaagag gaaaaagaaa agctaaagaa gcaaatataa
      121 gtgcaatttt acctattgat catttttttc atttatttta gatattattt ctaaattaga
      181 tttaattcct tctccacaaa ccttgcaata aagatcattc attgcaactt tacagccaca
      241 atttctacaa tacattttgt tctcctcttg cccctatatt ttatcattat tcctactttt
      301 tatcaatctt tttttatacc catagccagc accaaaataa aaactctaaa gctattactt
      361 tagagttgtt tttaatttaa attatttgtc tttttttgga aatctacgtg gtgggcgatc
      421 ttttttaact tcttctttgc ctttattagg gttttcaata gtatcttttc tagataaatc
      481 aattctacct ttttcatcaa taccaattac cttaacagct atttgatcac caattgcaag
      541 tacatcttca actttcgcaa ctttttcatg tgcaattttc gaaatatgaa gcaatccctc
      601 acagccttcc catagtctta aaaatgcacc atagctttca attctaatta ctgtaccttc
      661 atagatttgt cctacttcag cttcgcgaac gatattttca ataatttcaa ttgctttatc
      721 aatccactca tattcttgat gcattacaaa tactctacca tcttgttcga tatcaatctt
      781 aacattatta gttttttcaa taatttcgtt aattactttt ccaccagatc caataacatc
      841 tttaatctta tctggattaa ttctaatcat cttaacttta ggtgcataag gacttaactc
      901 tttacgaggt tcactaatag ttccaagcat atgttttaaa atttctaaac gacctttttt
      961 agcttgcgct aaagcttctt ttaggatttc agtagttact cctgagattt taatatccat
     1021 ttgtaaagca gtaatgccgt ctttagtacc agcaacttta aagtccatat caccaaaatg
     1081 atcttccata ccttgaatat cagttaaaat cgtataatcc ttaccatcca taattaatcc
     1141 catagctata ccagctacag gtgctttaat tggtacaccg gcatccatta aagccattgt
     1201 tcctgcacaa atagatgctt gtgatgatga gccatttgac tcaaagattt cacttacaac
     1261 cctaatagta taaggaaaag tgtcttcatc tggaataatg ttcattaaag ctttttctcc
     1321 taaagctcca tgaccaattt ctcttcttcc aggtccgcca taacgaccag tttcaccaac
     1381 actaaattga gggaagttat attgaagcat aaacctctta gattcttcac tagttaagcc
     1441 atcaataatt tggctttcag ctaaacttcc taaagtaact gttgtgaaag cttgagtttg
     1501 gcctcttgta aataaagcag atccatgtgg tcttgcaaca acatgaactc ttgatgataa
     1561 aggtctaatc tcatcagttt ttcttccatc aggcctaatc ttttcttttg taattagtct
     1621 tcttacctcg cctgatacaa tgttttcaca tgcttcttta gcaacttttt cagcttcttc
     1681 ttttgcttct acagtgtcag caagcgatac aaaataatct acagtttgct ttgttaactc
     1741 atcaatttta gctgctcttt cttgcttttc tacaattgat acagcttttc ttaattcctt
     1801 accaataaat ttttcaattt tagctactga ttcttcacca agtaagtcta agaaaacttc
     1861 agctttcttt aatccaactt ctttaacaac ttcttcttgg aatttaacta atcttttaat
     1921 ctcttcatga ccaaaaagta gagcttcaag cataacatct tcagaaatgt tttttgaact
     1981 agcttctacc atattaatag catctttagt accagcaata cttaaagcaa gttcagattt
     2041 tgcttgttct tcttcagttg ggttgataat aaatttacca tcaactaatc ccacttcaac
     2101 gccagcaatt ggtccattaa atggaatatt agaaatcatt aatgctagcg atgagccaag
     2161 cattgcagtc atcgcaggcg aattatctgg ttcactactt aaaacgatat taataatttg
     2221 cacttcatcc ttaaatcctt caggaaataa aggtcttatc gggcgatcaa ttaaacgagc
     2281 atttaatgtt tcaacttctg atgctcttcc ttctcttctt aaaaatccac caggaatttt
     2341 accagctgca tatagttttt cttgataaac aacagttaat gggaaaaatc cagcattaaa
     2401 accttcacta ttatgaacta ccgctgttag aacagttgta tcaccaaaat gtgtaactac
     2461 agctccatta gcttgtttag ccatttcacc tgtttcaaca actatcattc tacttccttg
     2521 cttaaattca aatgtttttt tcatatttat attctctcct tattttacat taaaaaaggg
     2581 taaatttaat taccccaaat tttattttct taaatttagt tttactaaaa cttcacggta
     2641 actgtttagg tttgttctct tcaaataatc taaaagattt cttctatgac caactttttt
     2701 aagtaagcca cgattagaat gatgatcttt tggatgaatt gcgaaatgtt catttaaacg
     2761 gttaatttca gccgttaaaa tcgcaatttg tacttctaca gaaccagtat cattttcgtc
     2821 tttaccgaat tctttaacta atttagcttt ttcttctttt gaaatacaag acatttttta
     2881 cctccttatt taattaactc aaataaacca agtgaaacgt tggagcatcg ttatacctag
     2941 tctaatgata atatattata acatatttta ttaattttgt aaagatattt ataaagataa
     3001 aactaattta taaacaaatt cactactctt aataattgcc tgttctaaat tcaaattgta
     3061 ctctttccaa ttagtttctg gtactaaatc agatattgtt cttatagcta caaaaggaat
     3121 atcaagtaaa taagatgcat gagcaaaagc agtactttcc atatcaaaaa ttgaaatgtc
     3181 atagtgacta aaatgatgat caataatagc tttaactttc ggataattat ctttatctac
     3241 aaaagtatca ctagtataac aacttacaaa tttataattt tctttaggaa aattatcttt
     3301 aattgtcttc tttaatttgc tatttaattc aaaatatctt ggacatttac tcatttgacc
     3361 ataaaaatac tcatgtttag gtagctctac atcaccataa aagtatttat caactactgc
     3421 caaatcaaat aaattcacct tatctgatac cccaccacaa acacctaaat tgattatata
     3481 atcaacttca acttgactta atagagttcc aatcaaaagc ccagcattag ttttaccaac
     3541 accacttttt actaaaacaa cctttttatt gtcaatatct ccataatgaa attcaaaatg
     3601 ataaacctct tcagtccacg aatttt
//
LOCUS       DAOSGS010000088         3622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_23726_length_3622_cov_5.428091, whole genome shotgun sequence.
ACCESSION   DAOSGS010000088 DAOSGS010000000
VERSION     DAOSGS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23726_length_3622_cov_5.428091"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ22_03825"
     CDS             complement(<1..346)
                     /locus_tag="PLQ22_03825"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOH59122.1"
                     /translation="MRYKAVISYDGTLFHGFQKQKKHRSVQEELENAIYKAFDQKKEV
                     YASGRTDKGVHALAQVIHFDLNLDLQRRNLKKAMNSFLPQDIYVNNVEKVDDNFHVRF
                     NAKAKTYRYLLDL"
     gene            complement(336..1340)
                     /locus_tag="PLQ22_03830"
     CDS             complement(336..1340)
                     /locus_tag="PLQ22_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH59123.1"
                     /translation="MGNKIVFGRYHNSNSWFHRLDPRTKLVGIFILMIALFIANNFWV
                     LLGAFGAILLLIITTKTPLLNFLKSIKGIAFILFFTIFFQIIFSRKGEMIWEGNFTLD
                     LFRILIVLILLIIFIFSKRLLPKARMLQFLVIVAVFFLLQYFPIFNLDFGNFLTKELA
                     TYKIMIYDGGLYTSLKIILRVMSILFISSLLTLTTKPEEITLGIEKLLSPLAKLKVNV
                     STFAMMISIALRYIPTLIVQANKILRSQASRGVDFVEAKLGEKIKQIISLLVPMFVIA
                     YNQAEDLANAMEARGYVPQNKRSSINVIKFRFIDGLILILIVGLLATTITLKAMGYAI
                     "
     gene            complement(1333..2202)
                     /locus_tag="PLQ22_03835"
     CDS             complement(1333..2202)
                     /locus_tag="PLQ22_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOH59124.1"
                     /translation="MGINFQDVKFAYYIPRNKKKTRYQLTDVNLNISAENEFIGIVGH
                     TGSGKSTLVQLMNALNRPTYGSVEVLGHKVTRKSKTLLKPIRQNVGLVFQFPEYQLFE
                     QTSLEDIMFGPKNFGFTKEEAEKNALEAAEIVGLNEELLEKNPFTLSGGEMRKVAISG
                     ILASKPKILIFDEPTVGLDPKTKKELMNLLLDLNKMGKTIIIVTHDMEVVSRYCDRVI
                     VLEEGKIALDLPKKELFSKKELLDRYSLDYPVLMKLLQRIKDQYNVDIDVNKFTLEDA
                     YLEIRKVLGKSHG"
     gene            complement(2178..2987)
                     /locus_tag="PLQ22_03840"
     CDS             complement(2178..2987)
                     /locus_tag="PLQ22_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOH59125.1"
                     /translation="MSIIEVKNLTFSYEKNKKIIDNLNLSIEKGSYVVILGHNGSGKS
                     TLAKLIVGLLEAQKGEIYIEGTKLSIDSIDDLRQRIGIVFQNPDSQFVGITVKDDIAF
                     GLENRNVPYEQMKEKVLEVAKLVGMENHLEKNPEELSGGEKQRVALAGVLVYDPEIVI
                     LDEATSMLDPKGVFEVNQAIQQFKHKKTIIAITHNLEEALYADRVIVLNQGKVVLDDI
                     PKRAFKEEEILKAANLDILESMKLIDLVKEDQDLSNKKEIEEILWELTFKM"
     gene            complement(2997..3425)
                     /locus_tag="PLQ22_03845"
     CDS             complement(2997..3425)
                     /locus_tag="PLQ22_03845"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOH59126.1"
                     /translation="MKKLDYFIIAIAFFLVIGSYTIYFANQDLANVNLILEVRYENKL
                     IYEVQYEEGLDEELTINQEGHIMTWSLKDGEEHTVVLESDKHIHNKVHLKYGEVKMTE
                     ANCKDKYCLQMQIKGRYSAPIICINGVSVILVGGELEIIF"
BASE COUNT         1239 a          655 c          391 g         1337 t
ORIGIN      
        1 ttaaatctaa taaataacga tatgttttag cttttgcgtt aaatctaaca tgaaaattat
       61 catcaacttt ctcaacatta tttacataaa tatcttgagg taaaaagcta ttcattgcct
      121 tttttaaatt ccttctttga agatctaagt ttaaatcaaa atgaataact tgagctaaag
      181 catgaactcc tttatcagtt ctcccagaag catagacttc ttttttttgg tcaaaggctt
      241 tataaatagc attttctaat tcttcttgaa cactacgatg tttcttttgt ttttggaagc
      301 catgaaacaa agttccatca taactaataa ctgctttata tcgcataacc catcgccttt
      361 aaagtaattg ttgttgcaag taaccctaca attaatatta atattaaacc atcaataaac
      421 ctaaacttaa ttacattaat tgatgacctc ttattttgag gaacataacc tcttgcttcc
      481 attgcattag ctaaatcttc tgcttgatta taagcaataa caaacatcgg aacaagtaaa
      541 gaaattattt gtttaatttt ctctcctagt tttgcttcca caaaatctac tcctcttgat
      601 gcttgactac gtaaaatctt attagcttgc acaatcaatg ttggaatata tcttaaagca
      661 atcgatatca tcattgcaaa tgtgctaaca tttaccttta attttgctaa aggcgataat
      721 agcttttcaa ttcctaaggt tatctcttct ggtttagtcg ttagcgttaa aagcgatgag
      781 ataaataaaa tagacataac acgtaagata attttaagtg atgtatataa accaccatcg
      841 taaatcataa tcttatatgt agctaattct tttgttagga aatttccaaa atctaaatta
      901 aaaattggaa aatattgcaa taaaaagaac acagcaacta tcactaaaaa ctgtaacatc
      961 cttgcctttg gtaataacct ttttgaaaag ataaaaatta ttaataaaat taaaacaatt
     1021 aagattctaa ataaatcaag tgtgaagttt ccttcccaaa tcatctcacc cttacgcgaa
     1081 aaaataattt ggaagaaaat cgtaaaaaac aaaataaatg ctattccctt gatacttttt
     1141 aaaaaattaa gaagtggtgt ttttgtagtg ataattaata ataaaattgc accaaaagca
     1201 ccaagtaata cccaaaaatt atttgcaata aacaatgcta tcattagaat gaagatacct
     1261 acaagtttag ttcttgggtc taaacgatga aaccaagagt tggagttgtg gtaacgacca
     1321 aatacaatct tattacccat gactcttacc taacactttt ctaatttcta aataagcatc
     1381 ttctaaagta aacttattta catcaatatc tacattatat tgatctttaa ttctttgtaa
     1441 cagtttcatt aaaacaggat aatctaaact atatctatct aataattctt ttttagaaaa
     1501 taactctttc tttggtaaat ctagtgctat cttaccttct tctaaaacta ttacacggtc
     1561 acaatatctt gaaacaactt ccatatcatg agtaacaata ataattgttt tacccatttt
     1621 atttaaatct aaaagtaaat tcatcaattc tttctttgtt tttggatcta aacctacagt
     1681 tggttcatca aaaatcaaaa tcttaggttt actagcaaga ataccagaaa ttgcaacttt
     1741 tctcatttcc ccaccagata aagtaaaagg atttttttct aatagctctt catttaaacc
     1801 aacaatttca gctgcttcaa gagcattctt ttctgcttct tcttttgtaa agccaaaatt
     1861 ctttggtcca aacataatat cttctagtga tgtttgttca aaaagctgat actcaggaaa
     1921 ctgaaaaact aaacctacat tttgcctaat cggctttaat aatgttttgc tttttcttgt
     1981 aaccttatga cctaagactt ccacacttcc ataagtaggc ctatttaatg cgttcatcag
     2041 ttggactaaa gtcgactttc ctgaacccgt atgacccaca atacctataa actcattttc
     2101 agcactaata tttaaattaa catcagttaa ctgataacga gttttctttt tatttctagg
     2161 gatataatag gcgaacttta catcttgaaa gttaattccc ataatatctc ctcaatttct
     2221 tttttattac ttaaatcttg atcttccttt acaagatcaa ttaatttcat actttctaaa
     2281 atatctaaat tggcagcctt taaaatctct tcttctttaa aagcacgttt aggaatatca
     2341 tctagtacta cttttccttg atttaaaaca ataaccctat ctgcatataa tgcttcttct
     2401 aaattatgcg taattgcgat aatagttttc ttgtgtttga attgttgaat cgcttgattc
     2461 acttcaaaaa cacctttagg gtctaacatc gatgttgcct catctaaaat tacaatttcc
     2521 ggatcataaa ctaaaacccc agctaaagca actctttgtt tttctccacc agataattct
     2581 tctggatttt tctctaaatg gttttccata cctactagtt tagctacttc taaaactttt
     2641 tctttcattt gttcataagg aacatttcta ttttctaaac caaaagcaat atcatcctta
     2701 acagttatcc ctacaaactg actatctgga ttttggaaaa caatcccgat tctttgcctt
     2761 aaatcatcaa ttgaatcaat gcttaattta gtaccttcaa tataaatttc tcctttttgt
     2821 gcttctagta aaccaacaat taacttagct aaagttgact taccagagcc attatgtcct
     2881 aagataacta cataagatcc tttttcaata cttaaattaa gattatcaat tattttttta
     2941 tttttttcat aactaaaagt taaatttttt acttcaataa tgctcattat tttcacctaa
     3001 aatattattt ctaactctcc gccaactaaa ataacagata ctccgttgat gcaaataatt
     3061 ggagccgaat accttccttt aatttgcatt tgtaggcaat atttgtcttt acaattagct
     3121 tccgtcattt taacttcacc atatttaagg tgaaccttat tatggatatg cttatctgat
     3181 tctaaaacaa ctgtgtgttc ttctccgtct tttaaagacc atgtcataat gtgcccttct
     3241 tggtttatgg ttagttcctc atctaaacct tcctcgtatt gcacttcata gattaacttg
     3301 ttttcatatc taacttctaa aattagatta acgttagcta aatcttggtt tgcaaaataa
     3361 attgtataac tgcctataac taaaaagaat gctatagcaa taataaaata atctaatttc
     3421 ttcattaaaa caagttctcc tttcttttac tgtataagta taccacattt agtgtttaaa
     3481 aactacttta ataataatga gtaaaacgtt ctttataatc caaacataaa cacaaaacgt
     3541 gctagcatta atagcataaa tacaattgtt gttatttgaa ccttaactcc tcggtctaaa
     3601 tctttattat aaacttttaa tt
//
LOCUS       DAOSGS010000089         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_24021_length_3591_cov_3.590215, whole genome shotgun sequence.
ACCESSION   DAOSGS010000089 DAOSGS010000000
VERSION     DAOSGS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24021_length_3591_cov_3.590215"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLQ22_03850"
     CDS             complement(<1..278)
                     /locus_tag="PLQ22_03850"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOH59127.1"
                     /translation="MRKRKDIIIAITETAIFVALAIVFELLSKLIGLSLPQGGSISLT
                     MLPLLIISIRRGVGYGLVAGLIFGIVNFFLDGFYWHWGSIFFDYLFAFP"
     regulatory      complement(335..432)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(454..1104)
                     /locus_tag="PLQ22_03855"
     CDS             complement(454..1104)
                     /locus_tag="PLQ22_03855"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH59128.1"
                     /translation="MKTWNDLFSIEKKKDYFLELAEFVDGEYEKYTIFPKKEEIFKAY
                     SLTPLENVKVVIIGQDPYHGENQANGLAFSCDYPILPPSLKNIYQELANDLKIRVPNK
                     GDLSKWAKEGVLLINAILTVRKNEPLAHENKGWEEFTKAVIEYLALYKKNVVYILWGA
                     YAQSYEQYINHQNNLILKSSHPSPLSAHRSFFGSRVFSKTNNYLKANGIKPIDWQL"
     gene            complement(1134..2504)
                     /locus_tag="PLQ22_03860"
     CDS             complement(1134..2504)
                     /locus_tag="PLQ22_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH59129.1"
                     /translation="MIPLAELKFNFITNNLWLDIVLICGLILSIILLGIYLVNDYAFQ
                     EASIAKLKSEDNGEKKGSKKAIFLAEKEDLTNISFFVLELFGVLLIAALTTVIAFSIF
                     DNIYLALLVVFFFVAIVLSIIFAVTYKLGREYSEKKILKYSNFIWIMTRITLPFTIFL
                     HFLTKKIKNKEKVAPEDGEVTDEKIEEIIDDIEDTGIFDEDDVELIQSALVLSAKTVY
                     DIMTPRVDMVALDQEMSNKEALEVFFEYQYSRIPVYKDDKDTIIGVLAERDFFTAIIK
                     EGIDNFKFKDLVVEPYYVSKNTKVDDLITEFQEAQRHFAIVLDEYGGTFGIVTMEDAL
                     EELVGEIYDEYDEVEDDEYLQLSKNKYLLDQGLDLQELFDELKLGTIPNTRYNTVGSF
                     VFSLCNGLPQVNQKVVHIQKVEDYEGEKPMLITYELKFKVLEVLERRIKKVELIIDKL
                     NEEPLI"
     gene            complement(2563..>3591)
                     /locus_tag="PLQ22_03865"
     CDS             complement(2563..>3591)
                     /locus_tag="PLQ22_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOH59130.1"
                     /translation="LLEKNCLVIATTHYSELKVYAYKAENVMNASVEFDVNTLKPTYK
                     LLMGIPGMSNALVISENLGLNKAVLSKAKANLKSKDSDLSQVLDTLTKKSLKVSEELD
                     SLKAQSKELEVKLKEVETLKLSALREKDRILSQAEKDAEKLLKENKQTLENLIKELEE
                     NNKKLIKEHEIADLKYKTKQIFQKADVVFEESDEELKVNDRVYIKSYNSYGTITKVLQ
                     KDLYEVMIGRLTSVLEKKYLKKVVQDDNTNLYKAKVSKVTPKRSVSSSLDLRGMRYEE
                     AENLLHDYFDQLLLSDLKQVTIIHGYGTGVIRKLVQSYLADNKNVLEFRYGGEKEGGM
                     GSTVVTLK"
BASE COUNT         1185 a          633 c          414 g         1359 t
ORIGIN      
        1 ggaaaagcaa acaaataatc aaaaaagatt gatccccagt gccagtagaa cccatctaaa
       61 aagaaattga cgattccaaa aattaatcca gctactaaac catatccaac gcctcttctt
      121 atactaatta taagtaaagg aagcattgta agacttatac ttccaccttg aggaaggctt
      181 aagccgataa gttttgataa taattcaaaa acgattgcaa gagctacgaa aatagctgtt
      241 tctgtaatag caataataat atcttttctt tttctcataa tttgctcctt aataataaaa
      301 aacctctaca caagtagaga ttaaaaacga tagttataaa aaactattta atccctacgc
      361 tagcattacc tagatcaggt tttagggtcg tatctaatac ctctcagcct taaggctccc
      421 ctttaaatta tacttaatta taccatattt ttcttataat tgccaatcaa ttggtttaat
      481 accatttgct tttaaatagt tattggtttt tgagaaaaca cgacttccaa aaaaagatct
      541 atgagcactt aatggtgaag gatgtgatga ttttaaaatc aaattgtttt gatgattaat
      601 gtattgttca tagctttgag cataagctcc ccataaaata tagactacat tcttcttata
      661 taaagctaaa tattcaataa cagcttttgt aaattcttcc caacctttat tttcatgagc
      721 taatggttca tttttcctta ctgttaaaat agcattgatt aaaagtactc cttctttggc
      781 ccattttgat aaatcgcctt tattagggac tcttatcttt aaatcattag ctaattcttg
      841 ataaatgttt ttaaggctag gaggtaatat tggataatca caagaaaaag caagaccatt
      901 tgcttgattt tcaccatgat atggatcttg gccgataatt acaaccttaa cattttctaa
      961 tggagttaag ctataagctt taaaaatctc ttcttttttg ggaaaaattg tatatttctc
     1021 atattctcca tctacaaatt cagctaattc caaaaaatag tcttttttct tttcaattga
     1081 aaacaaatca ttccaagttt tcatcctttg cctccttttt aaaaaccact tatttaaata
     1141 agtggttctt catttagctt atcaataata agttctactt ttttaatacg tcgttctaat
     1201 acttctagta ctttaaattt aagttcataa gtaattagca taggtttttc accttcatag
     1261 tcctctactt tttgaatatg aactactttt tgatttactt gaggtaggcc gttacataaa
     1321 ctaaagacaa agctaccaac agtattgtat ctagtattag gaatagttcc taactttaac
     1381 tcatcaaata gttcttgcaa gtctaaccct tggtctaaaa gatatttatt tttacttagt
     1441 tgtaagtatt catcatcttc aacttcatcg tattcatcat agatttcgcc tactaactct
     1501 tctaaagcat cttccattgt aacaatacca aaagttccgc catattcatc taaaacgatt
     1561 gcgaaatgtc tttgagcttc ttggaattcg gtaatcaaat catcaacttt agtatttttt
     1621 gatacataat atggctctac aactaaatct ttgaatttga aattgtctat tccttctttg
     1681 ataatagctg taaagaaatc tctttctgct agaacgccaa taatagtatc tttatcatct
     1741 ttgtaaactg gtattcttga atattgatat tcaaaaaaca cttccaaagc ttctttattg
     1801 ctcatttctt gatctaaagc taccatatca actcttggag tcataatgtc ataaacagtt
     1861 ttagctgata aaactaatgc agattgaatt aactcaacat catcttcatc aaagattcct
     1921 gtatcttcaa tatcatcaat tatttcttca attttttcat ctgtaacttc accatcttct
     1981 ggcgctactt tttccttgtt cttaattttc tttgttagga aatgtaagaa tatggtaaat
     2041 ggtaatgtaa ttctagtcat tatccagatg aaattagaat attttaagat ctttttctca
     2101 gaatattctc taccaagttt atacgttacc gcaaaaataa tactcaaaac aatcgctaca
     2161 aagaaaaaca cgacaagtaa tgcgagataa atattatcaa atattgaaaa agcaataact
     2221 gtagttaaag cagctattaa taatactcca aataattcta aaacaaagaa agaaatattt
     2281 gtaaggtctt ctttttccgc caaaaagata gcctttttac tgcctttttt ctcaccatta
     2341 tcttcagatt taagttttgc gatacttgct tcttggaaag cataatcatt tactaagtaa
     2401 atccctagta aaatgattga taaaattaaa ccacagatta aaacaatgtc taaccatagg
     2461 ttgtttgtaa taaaattaaa tttcaattcc gcgagtggaa tcactcttaa tcacctcttt
     2521 actttagtaa attataccaa aaatacacat tttatgcaat ctttatttta aagtaacaac
     2581 tgttgagccc ataccgcctt ctttttcgcc accatagcga aattctaaga catttttatt
     2641 gtcagctaaa taactttgga ctaactttct aataactcca gttccatagc catggataat
     2701 cgttacctgc tttaaatcac ttaataacaa ttgatcaaaa taatcatgaa gtaagttttc
     2761 tgcttcttca tagcgcattc cacgtaaatc taaactagaa cttactgatc tttttggagt
     2821 aactttactt actttagctt tatatagatt agtattatca tcttggacta ctttttttaa
     2881 gtacttcttt tctaaaactg aagttaacct tccaatcata acttcatata aatctttttg
     2941 caagacttta gtaattgtac cataagagtt gtaacttttg atataaaccc tatcatttac
     3001 ctttaactct tcatcacttt cttcaaaaac tacatcagct ttttgaaaga tttgtttagt
     3061 tttatattta agatcagcta tttcatgctc tttaattaac tttttattgt tttcttctaa
     3121 ttctttaatt aaattttcta aagtttgctt attttctttc aataatttct cagcatcttt
     3181 ttcagcttgg cttaaaatac gatctttttc tcttaaagca gataatttta aagtttctac
     3241 ttcttttagt tttacttcta attctttgct ttgggctttt aaactatcta attcttcact
     3301 cacctttagt gattttttag ttagtgtatc taaaacttga cttaaatcac tatcttttga
     3361 tttcaaatta gctttagctt tacttaaaac agctttattt aagcctaagt tttcagaaat
     3421 tactaaagca ttactcatgc caggaatccc cattaaaagc ttataagttg gttttaaagt
     3481 atttacgtca aattctacac tagcattcat gacattttct gctttgtatg cataaacctt
     3541 taactcagaa tagtgagttg tggcaataac aagacagttt ttctctaata a
//
LOCUS       DAOSGS010000090         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_24239_length_3569_cov_3.379055, whole genome shotgun sequence.
ACCESSION   DAOSGS010000090 DAOSGS010000000
VERSION     DAOSGS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24239_length_3569_cov_3.379055"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..402
                     /gene="ybeY"
                     /locus_tag="PLQ22_03870"
     CDS             4..402
                     /gene="ybeY"
                     /locus_tag="PLQ22_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006806742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH59131.1"
                     /translation="MKINLFLEVKEEFEFEDLIKKIAKLFKDKMSLSLIIVNDKQMRD
                     INFQYRDLDKTTDVLSFPDTEEDYLGDIFISLDKVKLQAVEYEHSEMEEFARLLIHGI
                     LHLMGYDHIKDEDHEVMVKKEEELLKKLFG"
     gene            416..823
                     /gene="cdd"
                     /locus_tag="PLQ22_03875"
     CDS             416..823
                     /gene="cdd"
                     /locus_tag="PLQ22_03875"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH59132.1"
                     /translation="MEKLYKEALKAFNNAYAPYSKFHVGAAVLLKDGTIVSGANVENA
                     SYGLSNCAERTALFKVYALGYRKDDIVKMLVLADTLEPVSPCGACRQVMSELLNEDTP
                     IILTNLKKVTKQYTIKELLPFSFKESDMDDAKK"
     gene            807..1706
                     /gene="era"
                     /locus_tag="PLQ22_03880"
     CDS             807..1706
                     /gene="era"
                     /locus_tag="PLQ22_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH59133.1"
                     /translation="MMPKSRSGFVTIIGRSNVGKSTFLNYVIGKKVAITTHKPQTTRN
                     VLRGVYNSEDAQIVFVDTPGVQKGKTALDKKMNKAAFTSLYDVDLILYMIDCNQKIND
                     EDKLILKSLKNLDTPKFLVVNKIDNLKNEAYMVENVLSLINELPFDEVFYISSLDGNN
                     VERMLNKAISYLPEGEPFFSEDTENLQSETFMISEIIREKILLLTHEEIPHSVGVIIE
                     SIKEDEEVEDLLVIEAKVIVEKASQKGIVIGKNGLMLKEIGTEARKEIVRTIKRKVYL
                     ELYVTVEPNWRSSDLGVKKAGYE"
     gene            1699..2418
                     /gene="recO"
                     /locus_tag="PLQ22_03885"
     CDS             1699..2418
                     /gene="recO"
                     /locus_tag="PLQ22_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH59134.1"
                     /translation="MSNLLEGLVLKEQKYQEDSKIAYILTNDGLKSYLVKGAYKVNAK
                     NASYSIPLTKISFDFSSKNNFNVLKQGKVLNNYSKIKANYELLLGALEVLDYTYDFAN
                     HVSDYDLFYQFVIFTLDKLEETGNLYYIAIFKLKLLYLLGIGPVLNRCVLCGSKENLI
                     GFSLNKAGLICKECNDYSLIKLDDFMKTIIVMYYTKLEDLNEEMFDLKYLDKINNLLD
                     QYYEIHLTYKSKANKVIKKIK"
     gene            2436..>3569
                     /locus_tag="PLQ22_03890"
     CDS             2436..>3569
                     /locus_tag="PLQ22_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOH59135.1"
                     /translation="MPLGMTKATHISEHKGHKELTKNQKILEYLDPKYVYFPAQRPDL
                     EYEVLVKVGDEVKIGDVLTISKAYFPVITHSSVSGKVTAVDKKVWHPSGRKISAIEIE
                     NNFKEEVTYLKEQPLTKEGIIETIRLAGIVGMGGAGFPTHVKYTHPKFQTDTLIVNAA
                     ECEPYLTCDCVSIKHRAKDLIQGIKYAMIANDAKRTVIAVKECHKDVIALLEEELAKE
                     ENISLYILKDVYPAGWEKYIVEKVQKKTYKGLPCETGAVVSNVSTLIAINDAVSKKMP
                     SVKRVITVSGEGIKNPQNVLVKIGTLASEIIDKTGGYADDLESGYLVAGGPMTGNAQF
                     FDSFMVEKTTTGLLVFKEQQGKEEACLGCGKCIDNCPVKLAPVL"
BASE COUNT         1348 a          384 c          682 g         1155 t
ORIGIN      
        1 ataatgaaaa ttaatctgtt tttagaagtt aaagaagaat ttgagtttga agatttaatt
       61 aagaaaatcg caaaactatt taaagataaa atgagtttat cattaataat tgtaaatgat
      121 aaacaaatgc gagatattaa ttttcagtat cgtgatcttg ataaaactac tgatgtttta
      181 tcttttcccg atacagaaga agattatcta ggagatatct ttatttcttt agataaagtt
      241 aaacttcaag ctgtagaata tgaacatagc gaaatggaag aatttgctag actattgatt
      301 catgggattt tacacttaat gggttatgat catattaagg atgaagatca tgaagttatg
      361 gtaaaaaaag aagaagaatt attaaagaaa ctttttggat aaagaggtaa aggatatgga
      421 aaaactatac aaagaagcat taaaagcatt taataatgct tatgctccat actctaagtt
      481 tcatgtaggt gcagctgttt tattaaaaga tgggacaatt gtaagtggtg ctaatgtaga
      541 gaatgcatct tatggcttat ctaattgtgc tgaaagaact gcgcttttta aagtttatgc
      601 tttaggctac agaaaagacg atattgtgaa gatgttagta ttagctgata ctttagagcc
      661 tgtttcacct tgtggtgctt gtaggcaagt tatgtctgaa cttttaaatg aagatacacc
      721 gattatcctt acaaatctta aaaaagttac taaacaatac acaattaaag aattattacc
      781 attcagtttt aaagaaagcg atatggatga tgccaaaaag tagatcaggg tttgtaacta
      841 ttattggtag aagtaatgtt ggtaaaagta cctttttaaa ttatgtaatt gggaaaaagg
      901 ttgcaattac aactcataaa cctcaaacaa caagaaatgt tttaaggggt gtttataata
      961 gtgaagatgc gcaaattgtg tttgtagata caccgggagt gcaaaaagga aaaacagctt
     1021 tagataagaa aatgaataaa gcagcattta cttcacttta tgatgttgac ttaattcttt
     1081 atatgattga ctgtaatcaa aagattaatg atgaagataa gttaatttta aaaagtttaa
     1141 agaatttaga tacaccaaag tttttagttg taaataaaat tgacaattta aaaaatgaag
     1201 cttatatggt agaaaatgtt ttgagtttaa taaatgaact accatttgat gaggtctttt
     1261 atatttcaag tcttgatggt aataatgtag agcgtatgct taataaagca attagttatt
     1321 tacctgaagg agaacctttc ttttcagaag atactgaaaa cttgcaatct gaaaccttta
     1381 tgatttcaga gattataaga gaaaagattt tacttttaac tcatgaagaa ataccacact
     1441 ctgtaggtgt gattattgaa agcattaaag aagatgaaga agtagaagat ttacttgtaa
     1501 ttgaggcaaa ggtgattgtg gagaaggcta gtcaaaaggg aattgttatt ggtaaaaatg
     1561 gcttgatgct taaagaaatt ggaaccgaag caagaaaaga gattgtaaga acaatcaaaa
     1621 gaaaagttta tttagagctt tatgttactg ttgaaccaaa ctggcgcagt tctgatttag
     1681 gagttaaaaa agcaggctat gagtaatctt ttagaaggct tagttcttaa agaacaaaaa
     1741 tatcaagaag attcaaaaat agcttatatt ttgactaatg atggtttaaa aagttattta
     1801 gttaaagggg cttataaagt aaatgcgaag aatgcaagtt attctattcc cctaacgaag
     1861 attagttttg actttagctc aaaaaataat ttcaatgttt taaaacaagg caaagtttta
     1921 aataattatt ctaagatcaa agcaaattat gaattattgt taggtgcttt agaggtatta
     1981 gattacactt atgattttgc taatcatgta agtgattatg atttgtttta ccagtttgta
     2041 atctttactt tagataagct agaagaaact ggtaatttat attatatagc tatttttaag
     2101 ttgaaactgc tatacttact aggaattgga cctgttttaa atagatgtgt tttatgtggt
     2161 tcaaaagaaa atttaattgg ctttagttta aataaagcag gattaatctg taaagaatgt
     2221 aatgattata gtttaataaa actagatgat tttatgaaaa ctattattgt gatgtattat
     2281 acaaagttag aagatctaaa tgaagaaatg tttgatttaa agtatttaga taagatcaat
     2341 aatttattag atcaatatta tgaaattcat ttgacttata agtcaaaggc aaataaagtt
     2401 atcaagaaga taaaatagag gaggaggaca attatatgcc attaggaatg acgaaagcaa
     2461 ctcatatttc tgagcataaa gggcataagg aacttacgaa gaatcaaaaa atcttggagt
     2521 atctagatcc taagtatgtt tactttccag cccaaagacc tgatttagag tatgaagttt
     2581 tagttaaagt aggagatgaa gttaagattg gtgatgtttt gacaatttca aaagcatact
     2641 ttccagtaat aactcattct tctgttagtg gtaaagttac ggcagttgat aagaaggtat
     2701 ggcatccatc tggaagaaaa atatcggcaa ttgaaattga aaacaacttc aaagaagaag
     2761 taacttacct aaaagaacaa cctttaacaa aagaaggtat tattgaaaca attcgtttag
     2821 cggggattgt ggggatggga ggagctggat ttccaactca tgtgaaatac actcatccta
     2881 aatttcaaac tgacacttta attgtaaatg cagctgagtg tgagccttac ttaacttgtg
     2941 attgtgtttc aattaaacat cgtgctaaag atttaatcca agggattaag tatgcaatga
     3001 ttgcaaatga tgctaagagg actgttatcg cagttaaaga gtgccataaa gatgttattg
     3061 cccttttaga agaagaacta gcaaaagaag aaaacattag tttatatatt ctaaaagatg
     3121 tttatcctgc tggttgggag aaatatattg tagaaaaggt gcaaaagaaa acttataaag
     3181 gattgccatg tgagactggt gctgttgtaa gtaacgtaag tactttaatt gcaattaacg
     3241 atgcagtatc taaaaagatg ccttctgtaa agagagttat cactgtaagt ggtgaaggca
     3301 ttaagaatcc tcaaaacgta ttagttaaaa ttggaacact tgcatctgaa attattgata
     3361 aaactggtgg atatgctgat gatttagaaa gtggatattt agtagccggt ggtccaatga
     3421 caggtaatgc tcaattcttt gatagtttta tggtagaaaa aactactaca ggactattag
     3481 tttttaaaga acaacaagga aaagaagaag catgtttagg gtgtggtaaa tgtattgaca
     3541 attgcccagt taaattagca cctgtttta
//
LOCUS       DAOSGS010000091         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_24483_length_3542_cov_3.457126, whole genome shotgun sequence.
ACCESSION   DAOSGS010000091 DAOSGS010000000
VERSION     DAOSGS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24483_length_3542_cov_3.457126"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="ruvB"
                     /locus_tag="PLQ22_03895"
     CDS             complement(<1..843)
                     /gene="ruvB"
                     /locus_tag="PLQ22_03895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH59136.1"
                     /translation="MEENELLNIDTDYDISLRPKKLVDFIGQADLKEMLHVFIQTALH
                     REETLDHVLLFGPPGLGKTTLASIIANEMGTNIYIVSGPSLDRVGDLAAILSSLDPGD
                     VLFIDEIHRIPRVVEEVLYSAMEDFAIDIITGKDSTATTLRIELPPFTLVGATTRVGD
                     ITAPLRDRFGIVQHLDYYNSEDLQKIIMRTAKVFNFKIAKDAAFEIAKRSRGTPRIAN
                     RLFKRVRDFAQFEDLDCKLITLDIANKSLEKLLIDSEGLNITDRRYLKTLIEKFNGGP
                     AGLNA"
     gene            897..1454
                     /gene="ruvA"
                     /locus_tag="PLQ22_03900"
     CDS             897..1454
                     /gene="ruvA"
                     /locus_tag="PLQ22_03900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH59137.1"
                     /translation="MYNYIKGKITYIGNGYIVLENNNIGYEIKSATPYDYKTGDEVIV
                     YLYLNVREDIQELYGFKTLEEKDFFLKLISVKGLGPKSALPIIASNNISGVIEAIKKG
                     DAKYLQKFPGIGPKASQQIILDLHGKIDIDKTIKTNPKLLQAKAALESLGYKQKEIKQ
                     VLPVIESNLDKELGDIIKIALRNLI"
     gene            complement(1484..2890)
                     /gene="ffh"
                     /locus_tag="PLQ22_03905"
     CDS             complement(1484..2890)
                     /gene="ffh"
                     /locus_tag="PLQ22_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH59138.1"
                     /translation="MAFESLSERLSMALRRITGRANLTEKDIDLMMREIRLSLLEADV
                     NYKVVRDFTKEVKEKALGEKIMKSLSPGDMVVKIVRDELQKLMGDEAAPLNYNLSGPT
                     VFMLVGLQGGGKTTHVGKLANYIRKQGDKKRPLFIAADIYRPAAIDQLKTIGKSLNIP
                     VFEMGTKEKVVKIVGEGIKYAKANKHDLVIIDTAGRLHIDDQMMDELIEVQKAVNPDE
                     VLLTIDAMMGQDAINVIETFNARLTITGCILTKLDGDARGGAALSVRYLTNIPIKFIG
                     VGEKLDDLEVFHPDRMADRILGMGDIVSLVEKTTQAVEEEEAEKMALKMQKGKYDYND
                     FLKQMKWIKRMGSLKGILKLIPGLGQQLKNIEFDDKQLAYIEAIIFSMTENERQNPEI
                     IARSSSRRTRISKGSGRPYPEVNALVRRFDTMKQQVEQLSRMDENQMKQMQRGGMPPM
                     PQAKKRKGKGKGKGNFRF"
     gene            complement(2901..3212)
                     /locus_tag="PLQ22_03910"
     CDS             complement(2901..3212)
                     /locus_tag="PLQ22_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOH59139.1"
                     /translation="MNLLEKNQHYIILYDFYGNLLTEKQQTYFKCYYFEDLSLGEISE
                     SYNVSRNAVYDSIQSTYKALDNFEEKLKLKAKYDARNKLYKKYNKEETKELIEKLKEL
                     E"
     gene            complement(3212..>3542)
                     /locus_tag="PLQ22_03915"
     CDS             complement(3212..>3542)
                     /locus_tag="PLQ22_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH59140.1"
                     /translation="VNLMKELEKIKRVISREVPNAPQDTLLVIDATTGQNGLNQAKSF
                     IEATDVSGIILTKLDGTAKGGIVLAIRDVYNIPIKFIGLGEKMDDLEYFDLEKYIYGL
                     FAELIEE"
BASE COUNT         1146 a          658 c          422 g         1316 t
ORIGIN      
        1 tgcatttaac cccgcaggtc ctccattaaa cttctcaatt aaagttttta aataacgtct
       61 atcggtaata tttaaacctt cactatcaat aagcaacttt tctaaagact tatttgcaat
      121 atctaaagta attagcttac aatctaaatc ttcaaactga gcaaaatccc ttactctttt
      181 aaataatcta ttagctatcc ttggtgttcc tctacttctt tttgctatct caaaagcagc
      241 atctttcgca atcttaaaat taaagacttt agctgtacgc ataataatct tttgtaagtc
      301 ttcgctgtta tagtaatcta aatgctgaac aataccaaaa cgatcacgta aaggtgctgt
      361 aatatcacct actcttgtag ttgcacctac taaagtaaaa ggtggtagtt ctattcttaa
      421 agttgtagca gtagaatctt taccagtaat aatatcaatt gcaaagtctt ccatcgcaga
      481 ataaagtacc tcttctacaa cccttggaat tctatgaatc tcatcgataa ataaaacatc
      541 acctggatct aaagaagata aaattgctgc taaatctcct acacgatcta aacttggacc
      601 agaaacaata taaatgtttg ttcccatctc attagcaata attgatgcta aagtagtctt
      661 accaagtcca ggaggaccaa atagtaaaac atggtctaaa gtttcctctc gatgaagagc
      721 cgtttgaata aagacatgca acatttcttt taaatcagct tgaccaataa aatcaactaa
      781 ctttttaggt cttaaactaa tatcatagtc agtatcaata tttaataatt cattttcttc
      841 catttttttc ctcctttttt ttagtaatct atggtataat aaataaggtg ataaatatgt
      901 ataattatat taaaggcaaa ataacttata tcggcaatgg ttacatcgtt ttagaaaata
      961 acaacattgg ctatgaaatt aaatcggcaa caccttatga ttataaaacg ggtgatgaag
     1021 taatagttta tctatatttg aatgttcgtg aagatattca agaactttac gggtttaaaa
     1081 ctttagaaga aaaagatttc tttttaaaat taatttccgt aaaaggttta ggtccaaaga
     1141 gtgccttacc tattattgct agcaataata tcagtggtgt tattgaggca attaagaaag
     1201 gtgatgcgaa atacttacaa aagtttcctg gcatcggccc taaagcttcc caacaaatca
     1261 ttcttgacct acacggtaaa atagatattg ataaaactat caaaacaaat cctaaacttc
     1321 ttcaagctaa agctgcttta gaaagtctag gctacaaaca aaaagaaata aagcaagtat
     1381 tacctgttat tgaatctaat ttagataaag aactaggtga tataatcaaa attgctttaa
     1441 gaaatttaat ttaaacttgt gtttttatac acaagttttt ttattaaaat ctaaagtttc
     1501 cttttccctt acctttacct tttctttttt tggcttgagg cattggtggc attccacctc
     1561 tttgcatctg tttcatttga ttttcatcca ttcttgataa ctgttctact tgttgtttca
     1621 ttgtatcaaa acgacgaact aaagcattaa cttcaggata aggtctacct gatcctttag
     1681 aaatccttgt tcttcttgaa ctactacgag caataatttc tggattttgt ctttcatttt
     1741 cagtcattga aaaaattatt gcttcaatat atgctaattg tttatcatcg aattcaatat
     1801 ttttaagttg ttgacctaaa ccaggaatta attttaaaat tccttttaaa gatcccattc
     1861 ttttaatcca tttcatttgc tttaggaagt cgttatagtc atacttaccc ttttgcatct
     1921 tcaaagccat tttttcagct tcttcttcct caactgcttg ggtagttttt tcaactaaac
     1981 taactatatc gcccattcct aaaatacgat cagccatacg gtctggatga aaaacttcta
     2041 aatcatctaa cttttctcca acaccgataa acttaatcgg aatgtttgta aggtaacgta
     2101 ctgataaagc agcaccacct ctagcatctc catctagttt tgttaggata caaccagtaa
     2161 tcgttaatct tgcattaaat gtttcgataa cgttaatcgc atcttgtccc atcattgcat
     2221 caattgtcag taatacttca tcaggattaa ctgccttttg tacttcaatt aactcatcca
     2281 tcatttgatc atcaatatga agtcttcctg cagtatcaat aattactaga tcatgcttat
     2341 ttgctttcgc atatttaata ccttccccaa ctatttttac aaccttctct tttgttccca
     2401 tttcaaaaac aggaatattt aaactcttac caattgtctt taactgatcg attgcagcag
     2461 gacggtaaat atcagccgct ataaatagtg gtcttttttt atctccttgt tttcttatat
     2521 aatttgcaag tttacctaca tgtgttgttt taccaccacc ttgtaagcct actaacataa
     2581 aaacagtcgg tccacttaaa ttataattta atggagcagc ttcatctccc attaattttt
     2641 gtagttcatc acgaacaatc tttacaacca tatccccagg agataaagat ttcataatct
     2701 tttcacctaa agctttttct tttacttctt tggtaaagtc tcgaactact ttatagttaa
     2761 cgtcagcttc aagtaatgaa agtctaatct cacgcatcat taaatctata tctttttctg
     2821 ttaagttagc tctaccagta attcttctta gagccatact taatctttct gataaacttt
     2881 caaaagccat ttcttacctc ctattctaat tcttttaatt tttcaattaa ttcttttgtt
     2941 tcttctttat tatatttctt ataaagttta tttcttgcat catatttagc ttttaatttt
     3001 aacttttctt caaaattatc taatgcttta taagtgcttt gaatactatc ataaactgca
     3061 tttcttgata cattataact ttcagaaatt tctcctaaac ttaaatcttc aaaataataa
     3121 catttaaaat atgtctgttg tttttcagtt agtaaatttc cataaaagtc atataaaata
     3181 atataatgtt gattcttctc taataaattc attattcttc tattaactcc gcaaataatc
     3241 cataaatata tttctctaaa tcaaaatatt ctaaatcatc cattttttca cctaaaccaa
     3301 tgaacttaat tgggatatta taaacatctc taattgcaag aacaatacca cctttagctg
     3361 ttccatctag tttagttagg ataatacctg aaacatcagt tgcttcaata aatgatttag
     3421 cttgatttag cccattttga cctgttgttg catcaattac aagtagtgta tcttgaggtg
     3481 cattaggtac ttcacgagaa ataactcttt taattttctc taattccttc atcaagttaa
     3541 ct
//
LOCUS       DAOSGS010000092         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_24710_length_3521_cov_4.714657, whole genome shotgun sequence.
ACCESSION   DAOSGS010000092 DAOSGS010000000
VERSION     DAOSGS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24710_length_3521_cov_4.714657"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..253)
                     /locus_tag="PLQ22_03920"
     CDS             complement(68..253)
                     /locus_tag="PLQ22_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59141.1"
                     /translation="MKNSIDSYPETHQFGRISIENLPENKLIEGDLGIQIADDGRVWV
                     CINGIAFIRFKPIKKEK"
     gene            complement(256..666)
                     /locus_tag="PLQ22_03925"
     CDS             complement(256..666)
                     /locus_tag="PLQ22_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOH59142.1"
                     /translation="MTKEWNKIFITPQKFHDDILKLVQLIPKDKYTLMVGIPRGGYII
                     AVYLSHYFDLTWMDFENFRKFQKYSNKESVLIVDDIADTGNTLKGFNKFDCATLYYKP
                     RSIVRPTYYIEEFKNSDWVVYPFEKPEEIPNREV"
     gene            complement(742..1239)
                     /locus_tag="PLQ22_03930"
     CDS             complement(742..1239)
                     /locus_tag="PLQ22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF039820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HOH59143.1"
                     /translation="MYWKYFKYVIKHKWFVFIECYKLRQIWRGITHDLSKFLPDEFIP
                     YARYFYGNWPSHKEVSQITHDNNYTKEGIQKAFDTAWLKHQHRNPHHWQYWLLQEDDG
                     DLKRLPMPMKYIEEMVADWKGAGRAITGINDSQECKNWYNKNKDKIRLNSCTEFYVKV
                     ILDNF"
     gene            complement(1310..1687)
                     /locus_tag="PLQ22_03935"
     CDS             complement(1310..1687)
                     /locus_tag="PLQ22_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOH59144.1"
                     /translation="MIHKFIKQAVEEANKSEHKQKVGAIIFDKSKIISKGFNHPQRSV
                     RHLKSKFQRWPGTVHAEVDAIIKAKTNLKNLSMLVVRVNSKNQLRMAQPCRWCQMYLE
                     YVGIKKVYYSIDEIPYINEIKIN"
     gene            complement(1684..2241)
                     /locus_tag="PLQ22_03940"
     CDS             complement(1684..2241)
                     /locus_tag="PLQ22_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59145.1"
                     /translation="MTSQEFQSKFKKGVNLLHQFDKCLYYNIECDCGDKSCGTTMTVE
                     CDTELGLIELHFYKDVQFDYWMYPEPGIVNYFKRLAYRWKKIIKLIFTGEISLESDFV
                     LIDIEHINNFIEAMQEGRDYCIKCQQERDKPKSNSQYECGASMTNCSYYLSDGRCGLG
                     NNNSPVSINQKCPNLKELNKKMLEL"
     gene            complement(2198..2392)
                     /locus_tag="PLQ22_03945"
     CDS             complement(2198..2392)
                     /locus_tag="PLQ22_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59146.1"
                     /translation="MFRQYNNVRIIGPVIFDAFDFFTKETYDQILAGNDTVVEELLFK
                     HVSGEGYDISRVSEQVQKRS"
     gene            complement(2417..3208)
                     /gene="folE2"
                     /locus_tag="PLQ22_03950"
     CDS             complement(2417..3208)
                     /gene="folE2"
                     /locus_tag="PLQ22_03950"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOH59147.1"
                     /translation="MSNNELPDVQLTKPKHEIAINRVGISDFKMPIFISEKDGGVQHS
                     VANISCYVDLAPNIKGISMSRIPIGLMKFAKEQLSSKMMIKISENIRRSTEADLCDLG
                     YYFTYFLTKHAPVSCEPGILPYEAWFNMIKTKDDHLFRFSVETIASSSCPCSKEISKY
                     SAHNQKSRIKITCQGRKDEFIWIEDIIKIAEEASSCEIYSVLKRPDEKVVTESMYENS
                     KFVEDIARSCYVKLSDIGSIEKFKVEVSNDESIHAHLAKAIIANY"
     gene            complement(3198..>3521)
                     /locus_tag="PLQ22_03955"
     CDS             complement(3198..>3521)
                     /locus_tag="PLQ22_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59148.1"
                     /translation="NIKIKQKRFFEILFSEPVDPKEPYFDSYLWFSIRDLLYVNEYQK
                     EYSPYRDKVHKVNEKLCDLLYDKIKKFMWEISKYMSDRVLSRELIKRELEIYLNNEGE
                     LNNVK"
BASE COUNT         1121 a          624 c          405 g         1371 t
ORIGIN      
        1 tagctcttgt cgaatccgtt gaacttaaaa atgggatagt gttcagactc actgacacaa
       61 tcatatctta tttctccttt tttatcggct tgaatcttat aaaagctata ccattgatac
      121 acacccaaac tctcccatca tcagcaattt gaatacctag atccccttct attaatttat
      181 tttctggtaa attttcaata cttattcttc caaattggtg agtttcagga taagaatcaa
      241 ttgagttttt cattatcaga cctctctgtt tggaatttct tcaggttttt caaaggggta
      301 tacaacccaa tctgaatttt taaattcttc aatataatat gttggtctta caatagatct
      361 tggtttatag tataaagtag cacaatcaaa tttgttaaac ccttttaatg tattaccagt
      421 atccgcaata tcatcaacga tcaatactga ttctttatta ctatatttct gaaatttccg
      481 aaaattttca aaatccatcc atgttagatc gaaataatga cttaaatata ctgcaataat
      541 atatccacct cttggtattc ctaccatgag agtatattta tctttaggaa ttaattgaac
      601 taattttaaa atatcatcat gaaacttttg tggtgttata aaaattttat tccattcttt
      661 tgtcattctt tccacctcca gatatgagat taaataaaat ataaattata aacatattat
      721 ttctatataa aaaatgtaaa attaaaaatt atcaagaatt acttttacat aaaattctgt
      781 acatgagttt aatctaattt tatctttatt tttattatac caatttttac attcttgtga
      841 atcattaatg ccagtgattg cccgaccagc acctttccaa tccgctacca tttcttcaat
      901 atatttcatt ggcattggta atctttttaa atccccatca tcttcttgta ataaccaata
      961 ttgccaatga tgggggtttc tatgttgatg ttttaaccaa gctgtgtcaa aagctttttg
     1021 aattccttct tttgtataat tattatcgtg agttatctga gatacttctt tatgagacgg
     1081 ccaatttcca taaaagtatc ttgcatatgg tataaattca tccggcaaga attttgaaag
     1141 atcatgtgta attcctctcc atatttgtct gagtttataa cattcaatga aaacaaacca
     1201 tttatgtttg ataacatatt tgaaatactt ccaatacatt tttattttcc tttcagtttt
     1261 gcggccggca agatctagtt taagatcttg ccggccattt ttagcgtctt taattaattt
     1321 ttatctcgtt gatatatggt atctcatcaa ttgaataata aactttttta ataccaacat
     1381 attctaaata catttgacac catctgcatg gttgcgccat tcttaattga tttttactat
     1441 taactcttac taccaacatc gacaaatttt taagattcgt tttcgccttt ataatagcat
     1501 caacttcagc atgtactgtt ccgggccatc tctgaaactt acttttcaaa tgccgaacag
     1561 atctttgggg atgattaaat ccttttgaaa ttatctttga tttatcaaaa ataatagctc
     1621 ccactttttg tttgtgttca ctcttattag cttcttcaac agcttgtttt ataaatttat
     1681 gtatcataat tcaagcattt ttttattcag ttctttaaga tttgggcatt tctgattaat
     1741 actaactggt gaattattat tacctaatcc acatcggcca tctgataaat aatatgaaca
     1801 atttgtcatc gatgctccac attcatactg tgaatttgat tttggtttat ctcgctcttg
     1861 ttggcatttg atacaataat ctctaccttc ctgcatagct tcaataaaat tattaatatg
     1921 ttcaatatca attaatacaa aatcgctctc caaagagatc tcgccggtaa aaattaattt
     1981 aataatcttc ttccatctat aagctaatct tttaaagtaa ttaacaattc ctggttctgg
     2041 atacatccaa taatcaaatt gaacatcttt ataaaaatga agttcaatta aaccaagttc
     2101 tgtatcacat tcaaccgtca ttgtagtacc acaagattta tcaccacaat cacattcaat
     2161 attataatat aaacatttat caaattgatg gagtaaatta actccttttt tgaacttgct
     2221 ctgaaactct tgagatgtca tatccttctc cagaaacgtg tttaaataat aattcctcaa
     2281 ctacagtatc attacctgcc aatatttgat cataagtctc tttggtaaaa aagtcaaatg
     2341 catcaaaaat tacaggccct ataattctta cattattata ttgtctaaac ataaaattac
     2401 tcacattcta tagaagttaa taatttgcaa taattgcttt tgctaaatgg gcatggattg
     2461 attcatcatt tgatacttct actttaaatt tttcaataga accaatatct gacaatttaa
     2521 cataacaact tcttgcaata tcttctacaa atttagaatt ttcgtacata ctctctgtga
     2581 cgaccttttc atccggacgt tttaataccg aataaatttc acaagaagat gcttcttctg
     2641 ctatctttat gatatcttct atccaaataa attcatcttt tcttccttga catgtaattt
     2701 taattctact tttttgatta tgagcgctat atttcgaaat ctctttggaa caaggacaac
     2761 tcgaagacgc aattgtttca acagaaaatc taaataaatg atcatctttt gttttaatca
     2821 tattgaacca tgcttcatac ggtaagattc caggctcaca actgactgga gcatgtttag
     2881 ttaaaaaata tgtaaaataa tatcctaaat cacaaagatc tgcttctgtt gatcttctga
     2941 tattttctga tattttaatc atcattttac tactcaattg ttctttagcg aatttcataa
     3001 gtccaattgg tattctagac atgcttatgc cttttatatt cggcgcgaga tccacatagc
     3061 aactgatatt tgctacacta tgttggactc caccatcttt ttctgatata aatatcggca
     3121 ttttaaaatc agagatgcca actctgttaa ttgcaatttc atgtttcggt tttgttagct
     3181 gtacatctgg taactcatta tttgacatta tttaattctc cttcattatt caaatatatt
     3241 tcaagttctc gtttaattaa ttctcgggat aaaactctat ctgacatata tttactaatc
     3301 tcccacataa atttcttgat tttatcatat aacaagtcac ataatttttc attgacttta
     3361 tgaactttat ctctatatgg tgaatactct ttttgatatt cgttaacata taataaatct
     3421 cttatagaaa accataaata tgaatcaaaa tagggttctt ttggatcaac tggttcacta
     3481 aatagaattt caaaaaatct tttttgttta atcttgatat t
//
LOCUS       DAOSGS010000093         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_25016_length_3489_cov_3.694525, whole genome shotgun sequence.
ACCESSION   DAOSGS010000093 DAOSGS010000000
VERSION     DAOSGS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25016_length_3489_cov_3.694525"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="PLQ22_03960"
     CDS             <1..2029
                     /locus_tag="PLQ22_03960"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOH59149.1"
                     /translation="AYILAELQTSPQTIAVGFLHDVLEDTEETKEDLTAKFGEDIVSM
                     VDGVTKIGKLKYMTKEKALVRSHQKILLAMAKDIRVVLVKLVDRVHNMRTLEHLPREK
                     QLRIAQETLDLYVPLAHRIGMYRIKAELEDTAFKYLEPETYQSVTKEITEQREVREED
                     IKRMSDALKVLLDDHHVKNYEIKGRLKNIYSIVKKMQTKQLRINEIYDLMALRILVPT
                     VEACYQVLGLVHGSWSPIPKRFKDYIATPKPNLYQSLHTTVVGIGGKVYEIQIRTFEM
                     DAVAEYGVAAHWAYKESRQGFTSEKAQMEVSSKLKWYRDLLEYVEMDGESDFDPLSDI
                     KEDIFSANVYVFTPKGDPLDFPQGSTPLDFAYRIHTEVGDKTVGAIVNGKIVPLSYKL
                     QTGDVIEIKTSKQSQGPNKSWLKLAKTRHARHKITSFLNKKQRDDFIKQGIDSWDRVA
                     KEERYNGPKLDDSLIESKFSKYKIDTVEDFYYEIGKNNISPKAAMNILSGDLDQKTTD
                     EILMQTINEKKTNLTRQTSALGVIVEGLDNAKIKLASCCFPLYGDEIIGYITKGRGIV
                     VHRANCPNISEHEMQRTIPLSWDTADNNHTYEIILTISSFNRNNIIAEIINVLNSMKV
                     YIKAISSKEINGGTDILTDVRVAIQDTKTLTNVIVNLQKISDIYEIKRGTR"
     gene            2026..2463
                     /gene="dtd"
                     /locus_tag="PLQ22_03965"
     CDS             2026..2463
                     /gene="dtd"
                     /locus_tag="PLQ22_03965"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008471325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH59150.1"
                     /translation="MRAVIQRAKDSKCVIEGKVTGKINFGYVVLVGFTEGDNLDTVKK
                     MAKKIANLRIFNDENGKMNKSILDVKGEILSISQFTLYANTNSGNRPSFTESLNPVDA
                     ENLYNLFNLELKNYGIKVEEGIFQSEMEISLTNLGPVTITLEF"
     gene            2528..>3489
                     /locus_tag="PLQ22_03970"
     CDS             2528..>3489
                     /locus_tag="PLQ22_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59151.1"
                     /translation="MSLKERYDALASMPLSLNDLPKLEDLVKLAKIQGENEISYLSRL
                     LIIDLYLENNMLDQAFEMASNAINNLNIINYPKIYASYLEEIIYICISKKNYKSAYRY
                     ALIKRNYLDDNNSDDVNRWYLEMSYIFAELDQKDKAITNLLVILKNNPSMELESLVLS
                     NLAKLYIDEKELGKAKDAIIKCLPLIKRLEDYEGLEYINYLNAKLYVLEDNYLMAKTQ
                     YQELFKERVELEPSFLGIGNEYLKLLLDLKEYELMFGFINKYRSSYLSSNDIYNKKLF
                     LEHNLLALVSNNMQYKNSFYSLIMQIMNLEQEIRKSENLLFHNI"
BASE COUNT         1312 a          424 c          597 g         1156 t
ORIGIN      
        1 tgcttatatt ttagctgaac ttcaaacttc ccctcaaaca attgctgttg gttttttaca
       61 cgatgtttta gaggatactg aagaaacaaa agaagatcta accgctaagt ttggtgaaga
      121 tattgttagt atggtcgatg gtgttactaa gattggtaaa cttaagtata tgactaagga
      181 aaaagcttta gttagatcac accaaaagat attattagca atggctaaag atattcgtgt
      241 ggttttagta aagctagttg atcgtgttca taatatgaga acattagagc acttgccaag
      301 agaaaaacaa cttagaattg ctcaagagac tttagactta tatgtacctt tagctcatag
      361 aatcggtatg taccgaatta aagctgaact tgaagataca gcatttaaat atttagagcc
      421 tgaaacttat caaagtgtca ctaaagaaat cacagaacaa cgcgaagttc gtgaagaaga
      481 tattaaaagg atgagtgatg cgcttaaggt attgttagat gatcatcatg taaagaacta
      541 tgaaattaaa ggaagattaa aaaatattta tagtattgtt aagaagatgc aaactaagca
      601 acttcgtatt aatgaaatct atgatcttat ggccttaaga attttagttc caactgttga
      661 agcttgttat caagtattag gtttagttca tggttcttgg agtccaattc ctaagaggtt
      721 taaagattat attgcaacgc caaaaccaaa cctttatcaa tcattacata caacggttgt
      781 agggattggt ggtaaagttt atgaaatcca aattagaact tttgaaatgg atgctgtagc
      841 tgaatatggt gttgccgctc actgggctta caaagagtca agacaaggat ttacttcaga
      901 aaaagcccaa atggaagtat ctagtaaact aaaatggtac cgcgacttac ttgaatatgt
      961 agagatggat ggcgaaagtg attttgatcc attaagtgat attaaagaag atatttttag
     1021 tgctaacgtt tatgtcttta cacctaaagg agatccactt gattttcctc aaggttcaac
     1081 acctttagat tttgcttatc gtattcatac cgaagttggt gacaaaacag taggagcaat
     1141 tgttaatggt aagattgtgc cattatcata taaactgcaa actggcgatg ttattgaaat
     1201 taaaacttca aaacaatctc aaggtcctaa taaatcttgg ttaaagcttg cgaaaactag
     1261 acatgctcgt cataaaatta cgtcattttt aaataaaaaa caaagagacg attttatcaa
     1321 acaaggaatt gattcgtggg atagagtagc taaagaagaa agatataacg gccctaaatt
     1381 agatgatagt ttaattgaga gtaagttttc taaatataag attgatacag tagaagattt
     1441 ctattatgaa attggtaaaa ataatatttc tcctaaggct gcaatgaata ttttaagtgg
     1501 cgatttagat caaaaaacta ctgatgaaat cttaatgcaa acaatcaatg agaaaaagac
     1561 aaatttaaca cgccaaacca gtgctttagg agttattgta gagggattgg ataatgcgaa
     1621 gattaaactt gcaagttgct gttttccact ttatggtgat gaaattattg gttatataac
     1681 taaaggaaga ggaatagttg ttcatcgtgc aaattgccct aatatttctg aacatgaaat
     1741 gcaaaggaca ataccacttt cttgggatac agcagataat aatcacacct atgagattat
     1801 cttaactatt tcttcattta ataggaataa tattatcgca gaaataatta atgtattaaa
     1861 ctcaatgaaa gtttatatta aagcaattag ttcaaaagag attaatggag gaactgatat
     1921 tcttactgat gtcagagttg caattcaaga cactaaaact ttaactaatg ttattgtaaa
     1981 cttacaaaag atttccgata tttatgaaat taaaagaggt acaagatgag agcagttatc
     2041 caaagagcta aagatagtaa atgcgttatc gaaggtaaag ttacaggcaa gattaacttt
     2101 ggttatgttg ttttagttgg ttttactgaa ggtgataact tagatactgt taagaaaatg
     2161 gccaaaaaga ttgctaactt aagaatcttt aatgatgaaa atgggaaaat gaataaatca
     2221 attttagatg ttaagggtga gattttatca atttctcagt ttacgctata tgcaaacact
     2281 aactcaggta atagaccttc atttactgaa agtttaaatc cggttgatgc agaaaactta
     2341 tataatttgt ttaatttaga acttaaaaac tatggaatta aagtagaaga aggtattttt
     2401 caaagtgaaa tggaaatatc tcttactaac ttaggtccag ttactattac actagaattt
     2461 taggatagaa tttttctatc ctttttttca tatatttatg atataatata gggtaaggag
     2521 aaatactatg agtttaaaag aaagatatga tgctcttgct agcatgcctt taagtttaaa
     2581 tgatttacct aaactagaag acttagtaaa acttgcaaag attcaagggg aaaatgaaat
     2641 tagttattta tctcgactat taattattga tctttatttg gaaaataata tgttagatca
     2701 agcttttgaa atggcaagta atgctataaa caatttaaac attattaatt atccaaaaat
     2761 ttatgcttct tatttagagg aaatcattta tatttgtatt tctaaaaaga actacaaatc
     2821 tgcttatcgt tatgctttaa tcaaaagaaa ctatttagat gacaataata gtgatgatgt
     2881 taatcgttgg tatttagaga tgagttatat ttttgcagag ttagaccaaa aagataaagc
     2941 aattactaac ttgctagtta ttctaaaaaa taatccttca atggagttag aatctttagt
     3001 attatctaac cttgctaaac tttatattga tgaaaaagaa ctaggcaaag ctaaagatgc
     3061 tattattaaa tgtttacctc taattaaaag attagaagat tatgagggct tagaatatat
     3121 caattattta aatgcaaaac tttatgtttt agaagataat tatttgatgg ctaaaaccca
     3181 ataccaagaa ttattcaaag aaagagttga acttgaacca agttttttgg gtattggtaa
     3241 tgaatattta aaactgttat tagacttaaa agaatatgag ttaatgtttg gttttatcaa
     3301 taaatatcgt tcttcatatt tatctagtaa tgatatttat aataagaaac tctttttaga
     3361 acacaattta ctagctttag tttctaataa tatgcaatat aaaaatagtt tttattcttt
     3421 aattatgcaa attatgaatt tagagcaaga aattagaaag agcgaaaact tacttttcca
     3481 taatattaa
//
LOCUS       DAOSGS010000094         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_25597_length_3435_cov_2.970118, whole genome shotgun sequence.
ACCESSION   DAOSGS010000094 DAOSGS010000000
VERSION     DAOSGS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25597_length_3435_cov_2.970118"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLQ22_03975"
     CDS             <1..216
                     /locus_tag="PLQ22_03975"
                     /inference="COORDINATES: protein motif:HMM:NF026954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HOH59152.1"
                     /translation="FKAKSAIHPNHIDIINNVYSPSKEEIVYAIQVLEMAKTNKGAFS
                     INNKMIDKPVIERAKKTIENAKAFGLI"
     gene            216..1721
                     /gene="citF"
                     /locus_tag="PLQ22_03980"
     CDS             216..1721
                     /gene="citF"
                     /locus_tag="PLQ22_03980"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOH59153.1"
                     /translation="MKNSLGRKIPKGYPLFESSKAFENKKRVLIPEVKTKNREKLVKS
                     YSEVFDQLGIKDGMTLSFHHHLRNGDQVLNACMEEVKKRNLKNLTIASSAIFPNNEIL
                     VELIRNKNVTNIYTNYLNGPVAEAISHGELEGILIMQTHGGRARSIEAGELKIDVAFI
                     ATPAATKEGDGSGVDGKNPCGVLGYPIPDMLYAKKKVVVTDTLVDKLTITDIKKEYID
                     YVLIVDSIGDSKGIVSGTTQVTFDPIGLKIAKDATQLLYELGVIKNGFSMQTGAGGTS
                     LAVASFVREKMLQEGIKGKMASGGITSYFVSMLEEGLIEEIRDVQCFDLEAIQSYKKN
                     EKHYLMSASKYANPYDEPVVNDLSFMILGATEIDLDFNVNVTTSSNGVIMGGSGGHSD
                     AAYGAEIALVVAKTMNARIPIIKKVVTTITTPGSDIDCLVTERGIAINPKRKDLLEKL
                     KMSKLNIVSIEELMNLTYQFTREPKPFEHKDRVVGLVEYRDGTIIDVLYQV"
     gene            1788..3089
                     /locus_tag="PLQ22_03985"
     CDS             1788..3089
                     /locus_tag="PLQ22_03985"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOH59154.1"
                     /translation="MGDKIIPRTKKDREQEELKAYMVDDNVYFVVHANVPYNLLDKYY
                     DDFLVEYFYLYLKKEFNVIGDKFVYDNDGSYFFLHLDSKDPIFLKQRTVRIEETHPLG
                     RLIDIDVYHKTTKSIRREDAGLKLRRKCFICERDSYQCRWEKTHTDLEYSTYIQTIIL
                     NFLKDKVQELSLKAMIYELDLDPKFGLVTKTSQGSHPDMDYNLMKNSAYFLSSKLKDY
                     VTLGFETKLRDNLYPDIKNLGKKHEKALLENAGGVNTYKGLHFLLGYTVTAFGFICSH
                     NVKLKDRYRGLINKIKISGQPLFFEFLGEPQTTGEKLFKAYGILGARGEVFYGLRTAQ
                     RALRVLEKYGNLSNEALTMALIEIVKSTTDTVLFARAKTEESYRYFKDLITSIKVYDE
                     EKIKEVTEECIKNNISIGGSADILVVAIFLYLLREEFFGRE"
     gene            3082..>3435
                     /locus_tag="PLQ22_03990"
     CDS             3082..>3435
                     /locus_tag="PLQ22_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOH59155.1"
                     /translation="MNKKIGVFDSGLGGITVLNYLKDFYPNYDFIYLADSLNCPYGEK
                     TSKEIENLVTKNTLFLESLGVELVVIACNTASANSGAVERFTQIPIVRIIKPTALEAL
                     KKTKTNNILVLATNLT"
BASE COUNT         1268 a          372 c          646 g         1149 t
ORIGIN      
        1 tttaaagcta aatcagcgat tcatcctaat catattgata ttattaataa tgtctattca
       61 ccttcaaaag aagaaattgt ttatgctatt caagttttag aaatggctaa gactaacaaa
      121 ggtgcgttta gtattaataa taagatgatt gataaaccag ttattgaaag agcgaaaaag
      181 acaattgaaa acgctaaggc atttgggtta atctaatgaa gaatagttta ggacgtaaaa
      241 tccctaaagg gtatcctttg tttgaatcat caaaagcttt tgaaaataaa aaaagagtct
      301 taatacctga agttaaaact aaaaatagag aaaagttagt taaatcttat tctgaagttt
      361 ttgatcaatt aggaattaaa gatggtatga ctttatcttt tcaccatcat ttacgtaatg
      421 gggatcaagt tttaaatgct tgtatggaag aagttaagaa aagaaattta aagaatttaa
      481 ctattgcatc aagtgcaatc tttcctaata atgagatttt agttgagtta attagaaata
      541 agaatgttac taacatctat actaactact taaatggacc agtagcagaa gcaataagtc
      601 atggagaatt agaaggaatc ttaattatgc aaactcatgg gggtagagca agatcaattg
      661 aagcgggtga gctaaaaatt gatgttgcct ttattgcaac tcctgctgct actaaagaag
      721 gtgatggtag tggtgttgat ggtaagaatc cttgtggggt attaggatat ccaattcctg
      781 atatgttata tgcaaagaaa aaagtggtgg ttactgatac tttagttgat aagttaacta
      841 ttactgatat aaagaaagaa tatattgact atgttttaat tgttgattca attggagata
      901 gtaaaggtat tgtaagtggt actacccaag taacttttga tccaattggt ctaaagattg
      961 caaaagatgc aacacagctt ctatatgaac taggtgtaat taaaaatggt ttttcaatgc
     1021 aaactggtgc tggtggaaca tcgctagctg ttgctagttt tgtcagggaa aagatgttac
     1081 aagaaggcat taaaggtaag atggcaagtg gaggaattac ttcttatttt gtttctatgc
     1141 ttgaagaagg acttattgaa gagataagag acgttcaatg ttttgattta gaagcaattc
     1201 aaagttataa gaaaaatgaa aaacattatc ttatgagtgc atctaaatat gctaatcctt
     1261 atgatgagcc agtagtaaat gatttaagtt ttatgatttt aggtgctacc gagatcgatt
     1321 tagactttaa tgttaatgta actacatcta gtaatggtgt gattatggga ggatctggtg
     1381 ggcattctga tgctgcttat ggtgcagaaa ttgctttagt tgtagctaag acaatgaatg
     1441 caagaattcc aattattaaa aaagttgtaa ctacaattac aacaccaggt agtgatattg
     1501 attgtttagt tacagaaaga ggaattgcga ttaatcctaa aagaaaagat ttactagaaa
     1561 aattaaaaat gagtaaatta aatattgttt caattgaaga attgatgaac ttaacttatc
     1621 agtttacaag agaacctaag ccttttgagc acaaggatag agttgttggt ttagtcgaat
     1681 atcgagatgg aacaataatt gatgtattat atcaagttta agaccttcgg gtctttttct
     1741 ttatttaggt tttgatttgt gttataatat tatataagga gataaaaatg ggcgataaaa
     1801 ttattccaag aacaaaaaaa gatcgtgaac aagaagaatt aaaagcttac atggtagatg
     1861 ataatgtcta ttttgtagtt catgcaaatg taccttataa tttacttgat aagtattatg
     1921 atgattttct tgtagagtat ttttatcttt atttaaaaaa agagtttaat gttattggtg
     1981 ataaatttgt ttatgataat gatggttcat attttttctt gcatcttgac tctaaagacc
     2041 caattttctt aaaacaaaga actgttagga ttgaagaaac tcatccatta ggaaggttaa
     2101 tagatattga tgtttatcat aaaacaacta aatcaattag aagagaagat gcggggctta
     2161 agttaagacg taaatgtttt atttgtgagc gtgatagtta tcagtgtagg tgggagaaaa
     2221 ctcatacaga tttagaatat agtacttata ttcaaacgat aatcttaaat tttcttaaag
     2281 acaaagttca agaattatct ttaaaggcaa tgatttatga gcttgatttg gatcctaagt
     2341 ttggtttagt tacaaaaact agtcaaggaa gtcatcctga tatggactat aacttgatga
     2401 aaaatagtgc ttatttttta tcaagtaaac taaaagatta tgtaacatta ggttttgaaa
     2461 caaaattacg tgataattta tatcctgata ttaaaaactt aggtaaaaaa catgaaaaag
     2521 cattattaga aaatgcaggt ggtgtaaata cttataaagg attacacttt ttattaggat
     2581 atactgttac tgcttttggc tttatttgct cacataatgt aaagctaaag gatagatata
     2641 gaggattaat aaataaaatt aagatttcag ggcaaccact tttctttgaa tttttaggtg
     2701 aacctcaaac tactggagaa aaactattca aagcttatgg aattttaggt gctagagggg
     2761 aagtttttta tggtttaaga actgctcaaa gggctttaag agtattagaa aagtatggga
     2821 atttatctaa tgaagctcta acaatggctt taattgagat agtaaaaagt actactgata
     2881 ctgtactttt tgcaagggct aaaacagaag aaagctatcg ttattttaaa gatttaatta
     2941 cttcaatcaa agtttatgat gaagaaaaaa ttaaagaagt tacagaagaa tgtattaaaa
     3001 acaatataag tattggtggg tccgccgata ttttagttgt agcgatattt ttatatttat
     3061 taagagagga gttttttggt cgtgaataag aaaattggtg tatttgattc aggattaggg
     3121 gggattacag tcttaaatta cttaaaagat ttttatccta attatgattt tatctacttg
     3181 gcagatagtt taaactgtcc ttatggagaa aaaacaagta aagaaataga aaatttagtt
     3241 acgaaaaaca cactcttttt agaaagttta ggagttgaat tagtagttat tgcttgcaat
     3301 actgctagtg caaatagtgg tgctgtagaa agattcacac aaattccaat tgttaggatt
     3361 ataaaaccaa cagccctaga agcattaaaa aagactaaaa caaacaatat tttagtctta
     3421 gctacgaacc ttaca
//
LOCUS       DAOSGS010000095         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_25613_length_3433_cov_3.623150, whole genome shotgun sequence.
ACCESSION   DAOSGS010000095 DAOSGS010000000
VERSION     DAOSGS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25613_length_3433_cov_3.623150"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1312)
                     /locus_tag="PLQ22_03995"
     CDS             complement(170..1312)
                     /locus_tag="PLQ22_03995"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOH59156.1"
                     /translation="MNIKLGDLCKIQAGGTPSRNISKYWENGDIPWVKIGDIESKFLN
                     KTTEFITKEGLNNSSAKMIEKGSILYTIFATLGEVCITNIDVTTNQAIAGLTLRDDKV
                     DLEYLYYFLKSLHLHVNSIGRGVAQNNINLTILREFSVPVPNRKKQLEIANILKVIEN
                     IIESRKKQIQEYDQLIKSRFVEMFGNPIKNDKEWKLKFLDEVCDVRDGTHDSPKYYNV
                     GYPLITSKNISNGNIDYSTAQLICEEDYQNINKRSKVDNGDILMPMIGTVGGAIIVRK
                     ERDFAIKNVALIKFNTKLVENTFIKYVLNSDQMNLHFEDLKKGGTQKFIGLKTIRNLP
                     IIVPPIKLQSQFATFVQQVDKLKFEVQKSLDESQTLFDSLMQEYFG"
     gene            complement(1323..2825)
                     /locus_tag="PLQ22_04000"
     CDS             complement(1323..2825)
                     /locus_tag="PLQ22_04000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOH59157.1"
                     /translation="MITGEVRNKIDGIWETFWTGGLTNPLSVIEQVTYLLFIKLLDDN
                     QIKKERNAHALGVQPKNLLFDSGNYVDEKENIDEPYSNLRWSVFKHFEPQELFNTVKD
                     KVFPFIKQINNGKDTAFSKYMNDAVFMVPTPRVLVKVVDGLDDLDLNNKDMMGDVYEY
                     LLAKIASSGKNGQFRTPRHIIRMMVEMMKPTLNDVIVDPAMGSAGFIVESARYINEHY
                     SKELLKAEHQSKYTTTMFSGYDTDQTMLRIGAMNMMLHGVDQPDIKYQDSLSEENTTS
                     NKFSLILANPPFKGSLDYEAVSKDLLAVTKTKKTELLFLAVMLRMLKVGGRAASVVPD
                     GVLFGSSNAHKAIRKELIDNHKLTAVISMPSGVFKPYAGVSTAIIIFTKTNTGGTDKV
                     WFYNMEADGYTLDDKRSPIEQNDIDDIINRFHNLDLEENRTRLDKSFFVDKKEIIAND
                     YDLSINKYLEVEYEKIEYDSPAVIMDRIDSLDDEIEVLKNELKSLLNLDL"
     gene            complement(2822..3022)
                     /locus_tag="PLQ22_04005"
     CDS             complement(2822..3022)
                     /locus_tag="PLQ22_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH59158.1"
                     /translation="MEYSKKIKLLREKLFISQKELADMLGVSFVTVNRWETGKFNPTI
                     KAKKKLNDLFVSNDINTLGGKK"
BASE COUNT         1089 a          572 c          449 g         1323 t
ORIGIN      
        1 catatgcagt atcaaatcga aataatttta aatgatatga gtgattcttg taattgtttc
       61 atttgagtga catttaaata atcactcata tcatttaaaa ttatttcgat ttgattctgc
      121 atatgatacg ctcctttttt aataggaagt atatcaaata tataagattt tatccaaaat
      181 actcttgcat taatgaatca aatagcgttt gtgactcatc gagtgatttt tgaacttcaa
      241 atttcaattt gtcgacttgt tggacaaatg tagcaaattg gctttgtagt ttgattggag
      301 gtacaattat tggtaagttt ctaattgttt tcaatccgat aaatttttgt gttccacctt
      361 ttttcaaatc ttcaaagtga agattcatct gatctgaatt taaaacatac ttgataaatg
      421 tattctctac taacttagta ttaaacttta ttaaagcaac attttttata gcaaaatctc
      481 gttcttttct tactataata gcaccgccaa ctgtcccaat cattggcatt aatatatcac
      541 cattatcaac tttagatctt ttattgatgt tttgataatc ttcctcacat atcaattgtg
      601 ctgtagaata gtctatatta ccatttgata tatttttaga ggtgattaac ggatacccta
      661 cattataata cttcggtgag tcatgagttc catctcgtac atcacatact tcatcaagaa
      721 attttagctt ccattcttta tcatttttaa tcggattgcc aaacatctca acaaatcggg
      781 attttataag ttgatcatac tcttgaattt gtttttttct gctctctatt atattttcaa
      841 ttaccttaag tatattagca atttctaatt gtttcttcct atttggaaca ggaacgctaa
      901 attctctcaa tatggttagg tttatattat tttgtgctac ccctctccct atgctattga
      961 catgtaaatg cagtgatttt agaaaataat aaagatattc taaatcaact ttatcatctc
     1021 ttagtgtcaa tccagcaatt gcttgatttg tagttacatc gatatttgta atacaaacct
     1081 cccctaaagt tgcaaatatc gtatacagta tgctcccttt ttcaatcatt tttgctgatg
     1141 aattattcaa gccttctttc gtaataaatt ctgttgtttt attaagaaat tttgactcaa
     1201 tatcacctat tttaacccat ggtatatcgc cattttccca atattttgat atattacggg
     1261 agggagttcc accagcttgt attttgcata agtctcctaa tttaatattc atagttttag
     1321 ccctacaaat ctaaatttag aagtgattta agctcatttt taagaacttc aatttcatca
     1381 tctaaagaat caattctatc cataattact gcaggtgaat catattcaat tttttcatac
     1441 tcaacttcta agtatttatt aatagataag tcataatcgt tagcaattat ttctttttta
     1501 tcaacaaaga atgatttatc aagcctagtt ctattttctt ctaggtctaa attatggaat
     1561 ctattaataa tatcatcaat atcattttgt tctattggac ttcttttgtc atctaatgta
     1621 taaccatcag cttccatatt ataaaaccat accttatcag tcccaccagt gtttgttttg
     1681 gtgaaaatga taattgccgt tgaaacaccg gcatatggtt taaaaacacc gcttggcata
     1741 gatataacag ctgttagttt atgattatca attagctctt ttctaattgc cttatgtgca
     1801 ttactagagc caaataaaac gccatcagga acaactgatg ctgctctacc tccaactttt
     1861 aacattctaa gcataactgc taaaaataag agttctgttt tctttgtttt ggttactgct
     1921 aaaagatctt tagatacggc ttcgtagtct agacttcctt taaatggagg attggctaaa
     1981 attaaagaga acttatttga tgttgtattt tcctctgaaa gtgaatcttg gtatttaata
     2041 tctggttgat caacaccatg aagcatcata ttcattgctc caattctaag cattgtctga
     2101 tcagtatcat agccactaaa catagttgtt gtatatttgc tttgatgttc agctttaagc
     2161 aattccttag aatagtgttc gtttatatat cttgctgatt caacaataaa acctgcactt
     2221 cccattgctg gatccacaat aacatcattt aatgttggtt tcatcatttc aaccatcata
     2281 cgaataatat gtcttggggt tctaaattgt ccattttttc cactagatgc tattttagca
     2341 agtaaatact cataaacatc acccatcata tctttgttgt taaggtcaag atcatctaat
     2401 ccatctacta cttttacaag tactctagga gtaggaacca taaacacggc atcattcata
     2461 tatttagaaa aagctgtgtc ttttccattg ttaatttgtt taataaaagg aaatacttta
     2521 tctttaactg tattaaataa ttcttgtggt tcaaaatgtt taaatacact ccatctaaga
     2581 ttgctatatg gttcatcgat gttttctttt tcatcaacat aatttcctga atcaaataac
     2641 aaattctttg gttgaacacc taaagcatgc gcatttcgtt ctttctttat ttgattatca
     2701 tctaaaagct taataaataa taaataagtt acttgttcaa taactgaaag aggattggtc
     2761 aatccgcctg tccaaaatgt ctcccatatt ccatcaattt tgtttctaac ttcaccagta
     2821 atcatttttt tcctcctaat gtattaatat cgttactaac gaataaatca tttagttttt
     2881 tctttgcttt gatagtagga ttaaatttac ctgtttccca tctatttaca gtcacaaaag
     2941 aaacaccaag catatcagct agttcttttt gggatataaa tagtttttct ctaagcaatt
     3001 taattttttt tgagtattcc attgaatatt ctcctaatca tattattaac attattatag
     3061 cactttttat ataaaaatat aaggttgttt aaatttattt aaaaatataa tgaaatttta
     3121 tcaaatttag taaaattatc cgccaattta cgccatgaag ggatgtcagc ctgtcactta
     3181 aatattaaaa agtaatcgtt aaattgtagg gtaatcgtta aattgtaata gttgaaaatt
     3241 aaaaaaacct aaaagttgat actttttagg tcttggtatg gtcggtcaag ttataagtgg
     3301 aaatggaata aggggatgta ttccctatct tcaaaataaa aaaagtttga cacaaattgt
     3361 atcaaacaaa tttctaatat ggtgcgggtg acaggaatcg aacctgcact ccgaagaact
     3421 agatcctaag tct
//
LOCUS       DAOSGS010000096         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_25691_length_3426_cov_3.096707, whole genome shotgun sequence.
ACCESSION   DAOSGS010000096 DAOSGS010000000
VERSION     DAOSGS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25691_length_3426_cov_3.096707"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLQ22_04010"
     CDS             complement(<1..821)
                     /locus_tag="PLQ22_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59159.1"
                     /translation="MFQRFKRGTLIIVFLLLGAFLFGCSCDDKNKEMPRTYVADGIYL
                     EYKTLDVNNRPLISTVAVTIKSDTIDSVYIDCIDSEEILVEGVVDYKLKAQSRKELGY
                     LFGLHNQDELASGYKKQDLNTTEGLQIYKDHLLAQNKKEWFEQAAILEAYFKTGNSVL
                     ELDNGYITSLEGVSIKDIAFSELFVKAVANAKTGTFKAFKYVNKYDVISATAKISKTK
                     KLEKLTLDVLSGKTSSGTFKFDLESRMTMKYAYGMYNKDDAEYEYVRQDLTTAIG"
     gene            complement(938..2023)
                     /locus_tag="PLQ22_04015"
     CDS             complement(938..2023)
                     /locus_tag="PLQ22_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH59160.1"
                     /translation="MRKLQKLVLVLVLMLGLGLLVGCSCEDGKKKLEDIQVSPAEVTL
                     DAGQIKELEVKPVPADAELPAVEFTSSDSTVASVGKDGKVLAVKAGTVEITVKAGTFT
                     KKVTVSVNPVLATALEVAATLALEVGAKATISYKISPQDVTNKVPSFESNNPAVASVN
                     AEGEVTGVAAGEAIIKVKADAIEKQVTVTVTAPVVERTYPCDGEFSAFEASLNYGAPM
                     YTMVTVKIENDEVVSFYIDALQSKKNEAGTNYDWNAKSKKELGYLYGMHNVNNADAGY
                     ERQDLSTEEGLAAYQAYLTEQGKLEWFEQANMITAYALENGLEGLVMDDATKKLDGSV
                     EALAGVSVTVEHYLAVLEAVYADFPQA"
     gene            complement(2139..2456)
                     /locus_tag="PLQ22_04020"
     CDS             complement(2139..2456)
                     /locus_tag="PLQ22_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOH59161.1"
                     /translation="MLLASAFGVFVLPAIILASIGLVFGIMIGFAEKYFHVEEDLRVQ
                     TVEKLLPGLNCGGCGYPGCPGFAKAIVEEGASPNKCKPLAADAKEQIVAYLKEELAKA
                     ESK"
     gene            complement(2458..3027)
                     /locus_tag="PLQ22_04025"
     CDS             complement(2458..3027)
                     /locus_tag="PLQ22_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOH59162.1"
                     /translation="MGSVLSLAFSAIIIQNVILSKFLGICPFLGVSKSKKSAVGMGVA
                     VVFVITVSSILAWVLYKYVLVQLEMIYMKTIVFILVIASLVQVIEMFLKKFSPALYKA
                     LGIYLPLITTNCAVLGMATLVAEGNYTFLEMFVYSFSSAVGFLLVIYIFSTIREKLNR
                     MEGVMKDIPIALITAGLMAMIFARFAGVI"
     gene            complement(3030..>3426)
                     /locus_tag="PLQ22_04030"
     CDS             complement(3030..>3426)
                     /locus_tag="PLQ22_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH59163.1"
                     /translation="LHQSLGIFIPLITVNCIILGRAEAFASKNSVLDSLLDGVGMALG
                     YTLAIAIVSIVREFLGTGGITIWGNLGFVLGKDLETGVSKFDFFTNFFTTPAGGFIVL
                     GIVFGVVALIKNKAKVKKEADKKKGAKEA"
BASE COUNT         1183 a          683 c          391 g         1169 t
ORIGIN      
        1 aaaccaattg cagttgttag atcttgtctt acatattcat attcagcatc atctttatta
       61 tacataccat aagcatattt cattgtcata cgtgactcaa gatcaaactt aaatgttcca
      121 gaactagttt taccagaaag aacatccaaa gtaagttttt ccaacttttt tgttttagaa
      181 atcttagccg ttgcactaat aacatcatat ttatttacat atttaaatgc tttaaaagtc
      241 cctgtcttag cattagcaac agccttcaca aataattcag aaaaagcaat atctttaata
      301 ctcacgcctt ctaaactagt aatataacca ttatctaact ctaaaacaga attaccagtt
      361 ttaaaatatg cttccaaaat agctgcttgt tcaaaccatt cttttttgtt ttgagctaaa
      421 agatgatctt tataaatttg caatccctca gttgtattta aatcttgttt tttatagcca
      481 ctagcaagtt catcttgatt atgaagccca aataaataac ccaattcttt tctactttgg
      541 gctttaagtt tatagtctac aacaccttca actaatattt cttcactatc gatacaatct
      601 atataaacac tatcaatagt atcacttttt attgtaacag ctactgtaga tattaatggt
      661 ctattattaa catctaatgt tttatattcc aaatagatac catcagctac gtaagttctt
      721 ggcatttctt tgtttttatc gtcacaacta caaccaaata aaaatgctcc aagcaataaa
      781 aaaactatta ttaatgttcc tcttttaaat ctttgaaaca taacatactc cttgttagca
      841 tattatatca caaacattca agtttttcaa tataaatata acaagaaaaa tcccaagaaa
      901 ccttgggatt tttatcttaa tttaaattat tccttaatta agcttgagga aaatctgcgt
      961 atactgcttc taaaacagct aaataatgtt caacagtaac actaacacct gctaatgctt
     1021 ctactgatcc gtctaatttc ttagtagcgt catccattac taatccttca agaccatttt
     1081 ctaaagcata tgcagtaatc atattagctt gttcaaacca ttctaatttt ccttgttcag
     1141 ttaaataagc ttgatatgca gctaaacctt cttcagttga aagatcttgt ctttcatatc
     1201 ctgcgtcagc attattaacg ttatgcatac catatagata acctaattct ttctttgatt
     1261 ttgcattcca atcatagttt gttccagctt cattcttttt gctttgtaat gcatcaatat
     1321 agaaacttac tacttcatca ttttcaattt taactgtaac cattgtatac attggagcac
     1381 catagtttaa actagcttca aatgctgaaa actctccatc acatgggtaa gttctttcaa
     1441 caactggtgc tgtaacagta acagtaactt gtttttcaat agcatcagct tttactttaa
     1501 taatagcttc acctgctgca actccagtta cttcaccttc agcattaaca cttgcaacag
     1561 ctgggttatt tgattcaaat gaaggaactt tgtttgtaac atcttgtgga ctaattttat
     1621 aactaatagt agctttagct ccaacttcta atgctaatgt agcagctact tcaagagcag
     1681 tagctaatac tgggttaaca gatacagtaa ctttcttagt aaatgtacct gctttaacag
     1741 taatctctac tgtacctgct tttacagcta atactttgcc atcttttcct acacttgcaa
     1801 cagtactatc tgaagatgta aattcaactg ctggaagttc agcatcagca ggaactggtt
     1861 ttacttctag ttctttaatt tgacctgcgt ctaaagttac ttctgctggt gatacttgta
     1921 tatcttctaa tttcttcttt ccatcttcac aactacaccc aacaagtaag cctaagccta
     1981 gcatcaaaac aagaactaat actaactttt gtaattttct catttttata tcctccttaa
     2041 atgatactta tattatacta tatgtgattg taaaaagcaa gtaaaaaatg aaaatacaaa
     2101 tgaaaataaa aggcctcagg ccttttattt ttttattctt acttactttc ggcttttgca
     2161 agctcttctt ttaaataagc tactatttgt tctttagcat cagcagctaa aggtttacat
     2221 ttattaggtg atgccccttc ttctactatc gcttttgcaa accctgggca tccaggataa
     2281 ccacaaccac cacagtttaa tccaggtagt aatttttcta ctgtttgaac tcttaaatct
     2341 tcttctacat gaaaatactt ttctgcgaac ccaatcataa ttccaaacac taaaccaata
     2401 cttgctaata taatcgcagg taagacaaaa acgccaaaag cacttgctaa taacatttta
     2461 aatcaccccc gcaaaccttg caaaaatcat tgccattaaa ccagcagtaa tcaacgcaat
     2521 cggaatgtct ttcataacac cttccatacg attaagtttt tctctaattg tagagaagat
     2581 ataaataaca agtaagaagc ctacagctga tgaaaagcta taaacaaaca tttctaaaaa
     2641 tgtataatta ccttctgcta ctagtgttgc catacctaat acagcacaat tagtagtgat
     2701 taaaggtaag taaataccta aagctttata taaagcagga ctaaatttct ttaagaacat
     2761 ttcaattact tgaactaaag atgcaattac taaaatgaac acaattgtct tcatataaat
     2821 catctctaat tgtactaaaa catatttata taaaacccaa gctagaatag atgaaacagt
     2881 aataacaaat actacagcta cacccatacc aacagctgat ttttttgatt tagatacacc
     2941 taagaaagga caaataccca aaaacttact taaaattacg ttttgaataa taatagctga
     3001 aaaagctaaa cttaaaacac tacccataat taagcctcct ttgcaccttt ctttttatca
     3061 gcttcttttt taaccttagc tttattttta attaaagcaa caacaccaaa aacaatacct
     3121 aaaacgataa atccaccagc tggagttgta aagaagtttg taaagaaatc aaatttactt
     3181 acgccagtct ccaaatcttt tcctaaaacg aaacctaagt taccccagat tgtaattcca
     3241 ccagttccta aaaactctct tacaattgat acaattgcga ttgctaaagt atagcctaat
     3301 gccataccaa caccatctaa tagtgaatct aaaacagaat ttttagaagc aaatgcttct
     3361 gctcttccta aaataataca gttaacagtt attaaaggaa taaaaatacc tagcgattga
     3421 tgtaag
//
LOCUS       DAOSGS010000097         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_26253_length_3374_cov_4.602892, whole genome shotgun sequence.
ACCESSION   DAOSGS010000097 DAOSGS010000000
VERSION     DAOSGS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26253_length_3374_cov_4.602892"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLQ22_04035"
     CDS             <1..427
                     /locus_tag="PLQ22_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOH59164.1"
                     /translation="DFLVSFNEGEPLKSLSKVASGGELSRFMLALKVMINEKMNYQTM
                     IFDEIDSGVSGKTAYAIASKLKQIANFSQVLSVTHLPQVAAIADSHLFISKEVLDGRT
                     ITKLKHLENEGRVIEIAKMISNGIVTDASYNLALELLKK"
     gene            complement(456..647)
                     /locus_tag="PLQ22_04040"
     CDS             complement(456..647)
                     /locus_tag="PLQ22_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59165.1"
                     /translation="MARKEKKEKEPVVVTKKRRDDSIEVEIKKSPAKTTLGKITIVLI
                     IIGIIVIPVVALIFLLSQA"
     gene            712..1923
                     /locus_tag="PLQ22_04045"
     CDS             712..1923
                     /locus_tag="PLQ22_04045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOH59166.1"
                     /translation="MQKGRIIFHIDMNAFFASCEIKRRPELKNKAVVVARNDEQRRAI
                     ILSPNYIARDKGIKTTMSVKKAMKIDPYIVVVEPDHAYYEEISRIFMKFLYLITDQIE
                     QGSIDEAYLDVTETSKKVEDPIMLAKYIQTTLNEKFDLPCSIGIGPNKFLAKMASNYK
                     KPLGITIFRKREIDKTIWPLDIGKMYGVGPKTKAKLNSIGINTIKDLAKYPDYNRLKT
                     VLGPSSADTLYNWAWGNDDSKVDPNRYEEVQSISNSNTFMYDVFDIRELKQMLKVLVN
                     TVSSRLDKEKKQAQTVSLQIKYGNFTSISRNKTMMIPVTESEEIYEIIEELFIENYDE
                     RQAVRLLGVGLMKLTPRVEKEIKQISFLDDLDEIESETQIDNLLKDVEEKFGKGIIKK
                     GYYKKDNVRKD"
     gene            1907..2758
                     /locus_tag="PLQ22_04050"
     CDS             1907..2758
                     /locus_tag="PLQ22_04050"
                     /inference="COORDINATES: protein motif:HMM:NF037635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOH59167.1"
                     /translation="MLGKIETYKVDRETDIGYTLIKDGKEYFLHRNETNFAKLKAKDL
                     IKGFLYLDKKGREAVTMIIPKVTVDDAGYGKVVSVNHSLGCFVDIGISKDILLSKDNL
                     PYNLDYWPEVDSLLLGILRVKKDKLYFNILRKDEIMSLPKNPQDLDYSVNYEGLVYFA
                     GEKGINLVTEDLNLIFIASENLRKNYQLGEKVEFRITEKLADKTYLGMLPGGVLSNIE
                     HDSKVILDYLKSHRGTMPYSSKTSPMIIERVFSMSKSSFKKALGNLYKKGEVELLDDK
                     TILKRTI"
     gene            2944..>3374
                     /locus_tag="PLQ22_04055"
     CDS             2944..>3374
                     /locus_tag="PLQ22_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOH59168.1"
                     /translation="MYQKVKGTIDYYGAEAKKLQIVSKKVKDIIENFGYEEVVLPIIE
                     NTEVFTRSSGETSDIVRKEMYTFEDRGKRSLTLRPEGTAGVVRFFLESKEYANLDLKK
                     YYYFGPMFRYERPQKGRYRQFIQFGVEVFGKSSYLLDVDVI"
BASE COUNT         1285 a          406 c          587 g         1096 t
ORIGIN      
        1 tgacttttta gttagtttta acgaaggtga acctttaaag tctttatcta aagttgcaag
       61 tggtggtgaa ttatcacgtt tcatgcttgc tttaaaagta atgattaatg aaaagatgaa
      121 ctatcaaacg atgatttttg atgaaattga tagtggtgtt tctggtaaaa ctgcttatgc
      181 aattgcaagt aaactaaaac aaattgccaa ctttagtcaa gttttatcag ttactcactt
      241 acctcaagta gctgctatcg ctgatagtca tttatttata agtaaagaag ttctagatgg
      301 aagaactata actaagttaa aacatttaga aaatgaagga cgagtgattg agattgcgaa
      361 gatgatctct aatggcattg taactgatgc tagctataat ctagcattag aactattaaa
      421 gaaataaaaa aagaagttat tatcagaaat aacttctatg cttgacttaa taaaaagatt
      481 aaagcaacaa ctggaattac aataatacca attattatta atacaattgt tatttttcct
      541 agtgtagttt tcgcaggtga ttttttgatt tcaacttcaa tactatcatc tctacgcttt
      601 tttgtaacta caactggttc tttttctttt ttctctttcc ttgccattaa tctcacctct
      661 tttattcttt gatacattat accataaaat tttgaaaaaa ggaatgataa tatgcaaaaa
      721 ggacgaataa tttttcatat cgatatgaat gctttctttg catcttgtga gattaaacga
      781 agacctgaac ttaaaaataa ggcggttgtt gtagcacgta atgatgaaca aagaagagca
      841 attattttat ctccaaatta tatagctaga gataaaggaa ttaaaacaac aatgtcagta
      901 aaaaaagcaa tgaaaattga tccttatatt gtagttgtag agccagatca tgcatattat
      961 gaagagattt cacgaatttt tatgaagttt ttatacttaa ttactgatca aatagagcaa
     1021 ggatcgattg atgaggccta tttagatgta actgaaacta gcaaaaaggt agaagatcct
     1081 attatgctag caaaatacat tcaaacaact ttaaatgaga agtttgactt accttgttca
     1141 attggaattg gtccaaataa gtttttagct aaaatggcat ctaactataa aaagccttta
     1201 ggcattacta tttttcgtaa aagagaaatt gataaaacta tatggccatt agatattgga
     1261 aagatgtatg gtgttggccc aaaaacaaag gcaaaattaa atagtattgg gataaatacg
     1321 attaaagatc tagctaaata cccagattat aaccgcttaa aaacagtttt aggtccatca
     1381 tcagctgata cactttataa ctgggcttgg ggaaatgacg atagtaaagt tgacccaaat
     1441 cgctatgaag aagtccaatc tatttctaac tcaaatactt ttatgtatga tgtttttgat
     1501 ataagagaac ttaaacagat gcttaaagta ttagttaata cggttagtag tagattagat
     1561 aaagaaaaaa aacaagctca aactgtgagc ttgcaaataa aatatggcaa ttttacatca
     1621 atttctcgta ataaaactat gatgattcca gttactgaaa gtgaagagat ctatgaaatt
     1681 attgaagaac tatttataga aaattatgat gaaagacagg ctgtaagact actaggagtg
     1741 gggctaatga aacttacccc aagagttgaa aaagaaatca agcaaatttc ctttttagat
     1801 gatttagatg aaatcgaaag tgaaacgcaa atcgataatt tacttaaaga tgtagaagag
     1861 aaatttggaa aaggaataat aaagaaagga tattacaaaa aagataatgt taggaaagat
     1921 tgaaacatat aaagtagacc gtgaaacgga tatcggctat actttgataa aagatgggaa
     1981 agaatacttt ttgcatcgta acgaaactaa ttttgctaag ctaaaagcta aagacttaat
     2041 taaaggtttt ttatatttag ataaaaaagg aagagaagca gtaacgatga ttattcccaa
     2101 agttactgtt gatgatgcag gttatggtaa agtagtttct gtaaatcata gcttaggctg
     2161 ttttgtagat atagggatta gtaaagacat ccttttatca aaagataatt taccatacaa
     2221 tttagattat tggccagaag ttgatagtct acttttaggt attttaagag taaaaaaaga
     2281 taaactttat tttaatattt tacgaaaaga tgaaataatg agtttaccaa aaaacccaca
     2341 agacctagat tatagtgtga actacgaagg attagtttat tttgcgggag aaaagggaat
     2401 taatttagtt acagaagact taaatttaat ctttattgct agtgaaaatt tacgtaaaaa
     2461 ttatcaatta ggtgaaaaag tggagtttag aattacggaa aagctagctg ataaaacata
     2521 tttaggaatg ctacctggtg gagttttatc aaatatcgaa catgatagca aagttatttt
     2581 agattactta aaaagtcatc gaggaacaat gccttattct tcgaaaacat cgccaatgat
     2641 tatagaaaga gttttttcta tgagtaaaag ttcatttaaa aaagctttag gtaatttata
     2701 taaaaaaggt gaagtagagc ttttagatga taaaacgata ttaaaaagaa cgatttaacg
     2761 taaatactaa tcttattgac aaatctcatc ttttatttta taataaagta tatgagaata
     2821 aaaacctaag ggcgctatta acgttctcta gttataaagt gcactatttg tttatgggta
     2881 gcacctttag tttatcgtcc ttaaatattt taaggacttt ttatttattc tagggaggat
     2941 attatgtatc aaaaagtaaa aggaacaatt gactattatg gcgctgaagc caaaaagtta
     3001 caaattgttt ctaaaaaagt taaggatata attgaaaatt ttggctatga agaagtagtt
     3061 ttaccgatta tagaaaatac tgaagtattt acaagatcat ctggtgaaac ttccgatatc
     3121 gtgagaaaag aaatgtatac tttcgaagat agaggtaaac gtagccttac tttgcgtcca
     3181 gaaggtacag caggtgtagt acgtttcttt ttagaaagta aagaatatgc taaccttgat
     3241 ttaaagaaat attattactt tggaccaatg tttcgttatg aaagaccaca aaaaggtaga
     3301 taccgccagt ttattcaatt cggtgttgaa gtttttggaa aaagcagtta tttacttgat
     3361 gttgatgtta ttta
//
LOCUS       DAOSGS010000098         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_27216_length_3291_cov_4.207046, whole genome shotgun sequence.
ACCESSION   DAOSGS010000098 DAOSGS010000000
VERSION     DAOSGS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27216_length_3291_cov_4.207046"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1012)
                     /locus_tag="PLQ22_04060"
     CDS             complement(197..1012)
                     /locus_tag="PLQ22_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOH59169.1"
                     /translation="MIKKIGIIGLGTIGSKLVVYLSSKGVEILAYCYRNLEIHKEQIK
                     NILKKDGNNTLNSLPIIITNDLVSLRDVDLIIDATTEDYQVKKNLYKNISSLGNNDII
                     IGCTTSSLNLNKLASFYQPENFFGLHFFNPPDKMKLIEIAYNFNISPKCALIIEDIKN
                     MLTDKVVIELPMIQGYVVNRILFAYLNYAYNFQLDTKLDYNIIDNCMKLGTNVPMGPF
                     QLSDYIGIDVCFTILNELYNSLQNEIYKPSLLLYEFIEKGYLGKKTKIGFYTY"
     gene            complement(1023..1655)
                     /locus_tag="PLQ22_04065"
     CDS             complement(1023..1655)
                     /locus_tag="PLQ22_04065"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOH59170.1"
                     /translation="MERVLILSPHTDDAELGCGGLICKLLEKKSELYWIVFSTAEESL
                     PKSYPNDTLIKEFISVTNYLGLCNTKYQIEHFKVRKLAEKRQEVLEILVNIRNIYNPT
                     LVIGPSLHDYHQDHLVVANEMIRAYKMTSSIICYELPWNQVSFNSQFFVKLDEKYIQG
                     KIKMLSYYKSQKSQNKSYFSEDFIRGLASTRGTQIGCRYAEAFEVIRWII"
     gene            complement(1662..2588)
                     /locus_tag="PLQ22_04070"
     CDS             complement(1662..2588)
                     /locus_tag="PLQ22_04070"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOH59171.1"
                     /translation="MYHYEDIKTLVDKETVFIGDLSNIEFDKAKNLNDADEYSIVWVK
                     NSGQDAYDKISQTKARVIICKNNIDLDKKILDRKCCVLTSNPKYIFTKIVNHLFVPKW
                     ETGIHQTAIIDKEAKFGKNVYIGPYSVIEKCILGDNCYIDSHCHIYNNVIIGNNVQIG
                     SSTIIGGFGFGYSRDENEKLHRFPHIGGVVIGDDVEIGSNTSIDCGSLSDTIIKNGVK
                     IDNLVHIAHNVIIEKDSLIIAHSILCGSCKIGERTWIAPSVCVRNNVVIGNDVVVGMG
                     AIVTKNIPDNESWIGNPAKPLSEYLLSKGEGK"
     gene            complement(2642..>3291)
                     /locus_tag="PLQ22_04075"
     CDS             complement(2642..>3291)
                     /locus_tag="PLQ22_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HOH59172.1"
                     /translation="KYPGYHELAYLHPNVFTPDESVLDILGVKKGEKYAIVRFVAWKA
                     SHDIGHSGISYENKIKLVKTLSRHLHVFISSEMELPEELKNYQIKIPPEQMHNALAFA
                     HLFIGESGTMASESAVLGTPNILIGPRAPFIGIHKELSNRFKLQHIFIDIQEAWELIN
                     YFARSNNPKEEMKNKVDILYKNKIDVTAFLVWFVNNYPESINEAKNSEFVFSSFH"
BASE COUNT         1112 a          555 c          385 g         1239 t
ORIGIN      
        1 gccttcggtg tactcggtgg ctttatttaa atctaccaaa atcaaaatcc ttttccttta
       61 cttcttttac cgattcagga taattttcta caaaccagac aaatctttct aatctatatc
      121 tttattaaat aattaaatat ccaagaaatg ggtatgcttt gataaatgaa atgattttta
      181 gccataataa tctgatttaa tatgtataaa atccaatttt tgttttttta cctaagtaac
      241 ctttttcaat aaattcatat aataataagc taggcttata aatttcattt tgcagagaat
      301 tatataattc atttaaaatt gtaaaacata catcaattcc tatataatca gacaattgaa
      361 agggacccat tgggacattt gtaccaagtt tcatacaatt atcaataata ttataatcaa
      421 gttttgtgtc taattggaaa ttgtaggcat agtttaaata agcaaaaagt attctattaa
      481 caacataacc ttgaatcatt ggtaattcta ttactacttt atcagttaac atattcttta
      541 tatcttctat tattaatgca catttaggag atatattgaa gttatatgcg atttcaatta
      601 gtttcatctt atcaggagga ttaaaaaaat gtaatccaaa aaaattctca ggttgataaa
      661 aactagccag tttatttaaa tttaaacttg aggttgtaca acctattatt atatcattgt
      721 tacctagtga acttatattt ttatacaggt ttttttttac ttgataatct tcagtagtcg
      781 cgtcaataat tagatcaaca tctcttaatg atactaaatc atttgtaatt ataataggca
      841 atgaatttaa tgtattatta ccatctttct ttagtatgtt tttaatttgt tctttgtgta
      901 tttctaaatt ccgataacaa tatgctaata tttcaacccc tttgctagat aaatacacaa
      961 ctaattttga ccctattgtc cccaacccaa taattcctat tttttttatc attttcacct
     1021 ccttatataa tccatcttat tacttcgaac gcttccgcgt atcggcatcc gatttgagtt
     1081 ccccttgttg acgctaaacc ccttataaaa tcttctgaaa aataactttt attttggctt
     1141 ttctgagatt tataatagct caacatttta atctttccct gtatatattt ttcatctaat
     1201 tttacaaaaa attgagagtt gaaagatact tggttccatg gcaattcata acagataatg
     1261 ctgctagtca ttttataagc acgtatcatt tcattagcga ctactaaatg atcttgatgg
     1321 taatcatgta aagatggacc tattactagt gttggattat atatatttcg tatattaaca
     1381 agaatctcta atacttcttg tcttttttct gcaagtttac gaactttaaa atgttctatc
     1441 tgatattttg tattacataa acctaaataa ttagtaacag aaataaactc ttttattaat
     1501 gtatcattgg gataactttt aggtaatgac tcttcagcag tagaaaagac aatccaatat
     1561 aattcacttt ttttctctaa cagtttacat attaatcctc cacaaccaag ctcagcatca
     1621 tcagtatgag gagataagat taatactctt tccataattt tctatttacc ctcaccttta
     1681 gataataagt attcagataa tggcttagca ggattaccaa tccagctctc attatcgggg
     1741 atattttttg ttacaattgc tcccatgcct acaacaacat catttcctat tactacatta
     1801 tttcttacac atactgatgg agctatccaa gttctttctc caattttaca actaccgcaa
     1861 agaattgaat gagcaataat taaagaatct ttttcaatta taacattgtg agctatgtga
     1921 acaagattat caattttaac accatttttg ataatagtat cgcttaatga tccacaatca
     1981 atagaggtat ttgatccaat ttctacatca tcaccaatta ctacaccacc aatatgagga
     2041 aacctatgca acttttcatt ttcatcacga ctatagccaa aaccaaatcc tccaataata
     2101 gtagaagatc caatttgaac attattccct atgataacat tattataaat atgacaatga
     2161 gagtcaatat aacagttgtc acctagtata catttttcta taacagaata aggtccgata
     2221 taaacatttt tcccaaattt agcttcttta tctataattg cagtttgatg gatacctgtt
     2281 tcccacttcg gaacaaataa gtgattaaca atttttgtaa atatatattt aggattgctt
     2341 gttaatacac aacactttct atctaatatc tttttatcta gatcaatatt atttttgcat
     2401 ataataactc ttgctttggt ttgagagatt ttatcgtatg catcctgtcc actatttttt
     2461 acccaaacaa tagaatattc atctgcatca ttcaaattct ttgctttatc aaattctatg
     2521 tttgataaat caccaataaa aaccgtctct ttatcaacta aagtttttat atcttcataa
     2581 tgatacataa atatgtactc ctatatttta atatctatat gttattttac tcagcactgc
     2641 atcaatgaaa agaagaaaag acaaattctg aatttttagc ttcatttata gattcagggt
     2701 aattattaac aaaccaaact aaaaaagctg taacatctat tttattttta taaagtatat
     2761 caactttatt tttcatttct tctttaggat tgttggaacg agcaaaataa ttaatcaact
     2821 cccatgcttc ttggatatct ataaatatat gctgcaattt gaatctatta gataattctt
     2881 tatgaatccc aataaatgga gcacgtggac cgattaatat atttggagtt cctaaaacag
     2941 cactctcaga agccattgtt ccactttctc caataaatag atgagcaaaa gctaaagcat
     3001 tatgcatttg ttcaggagga attttgatct ggtaattttt caactcttcc ggtaattcca
     3061 tttctgaaga gataaagaca tgtaagtgtc gggaaagtgt tttaactaat tttattttat
     3121 tctcataaga aataccacta tgacctatat catgagatgc cttccaggca acaaatctta
     3181 caattgcata tttttcacct tttttcactc ctaaaatgtc taagacactt tcatcgggag
     3241 tgaatacatt gggatgtaaa taagcaagtt catggtagcc aggatattta a
//
LOCUS       DAOSGS010000099         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_27734_length_3249_cov_4.697245, whole genome shotgun sequence.
ACCESSION   DAOSGS010000099 DAOSGS010000000
VERSION     DAOSGS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27734_length_3249_cov_4.697245"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2039)
                     /locus_tag="PLQ22_04080"
     CDS             complement(<1..2039)
                     /locus_tag="PLQ22_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH59173.1"
                     /translation="MRVKEIEIKDYNCLVCNDEINLILQNNKISSKKISGNKILFSSE
                     DDGKLKKADIEIQKVLQKHGHSFKTDEYVSYSFNLRNVTCPSCSRKIKRDLLKDNNII
                     ESEILIETQTLKLKLKEKENEKQLVRRINNIIKNYITNGSIVLEKKRNFVKDNWLLIS
                     RILVATILLLIGSFVFKDQTYKLVTFILSYLIISYNIIYRAFKALFSKFSLNEHFLMM
                     VASIGSFIINEPTDSILVMLLYQVGELLKDLSINRSTKSIQELLAFTEKEVNVIVNDE
                     VIRMDIGDVKIDSIVLVKTGEKVLLDGIIVDGEGYLNTKMITGESKQLQAIKGEEVVS
                     GYILESGTLRIKTTSDASNSKINQIISFLEEAKENKSETELRVSKMAKYYTPIILLLA
                     VLIFIIPVSILGFTTTSLETWGYRALIFLVVSCPCSIVISVPLAYFLSVGAASRKGIL
                     IKSGNSLDVLENIKTVYLDKTGTMTHGVLRVSKIHTIDYSKDEFFELVALSEIKSHHP
                     IAKSIVSEYRDKLDLQRVKMFEEIKGQGIKTIVDDKSIIIGSASFLKENGIKLEEVET
                     VGSIIYVSINNKYSGYIVIQDELRKDAKHLISFLKKKKIKTHLLTGDNETEAKRITGI
                     LEIDDYNHSLLPLDKTEIIKQDTSKTLFLGEGINDAPSLEISDLGVSLGGSASDLA"
     gene            complement(2029..2355)
                     /locus_tag="PLQ22_04085"
     CDS             complement(2029..2355)
                     /locus_tag="PLQ22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH59174.1"
                     /translation="MNVDKIKNKIITPDEQEKITLLFRTMGDSTRFTIIIALFESELS
                     VTELTNITGMNQSAVSHQLRVLKEQDIVRFKRQGKKRIYYLADHHIKTLYELALIHVR
                     EEKDES"
     gene            complement(2455..3123)
                     /locus_tag="PLQ22_04090"
     CDS             complement(2455..3123)
                     /locus_tag="PLQ22_04090"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOH59175.1"
                     /translation="MYKLGIIGGMGPLATSELFRRIVLKTDAKFDREHIKIAILNNPS
                     IPDRTQAIVAGGNSPVPYLNEAIADLKKLGAEYVIMPCNTAHYFVDKVDFQGLKFINM
                     VEETLNYLKEKYNDKKIIVLSTSGTNVANVYRSKDLNISYADYMSQKSLDQFIYQIKE
                     GYDLEAIAIRLKKMLADKSIVYVLACTELSLLKEYLKEYIIVDAMDILVLKTIEKLGY
                     QYKK"
BASE COUNT         1089 a          551 c          330 g         1279 t
ORIGIN      
        1 gcaagatcac tagcagaccc acccaaacta acccctagat cactaatttc taaactagga
       61 gcatcattta ttccttctcc taaaaatagt gttttactgg tatcttgttt aataatttct
      121 gttttatcta aaggtaataa tgaatgatta taatcatcaa tttctaaaat tcctgtaatt
      181 cgtttagctt ccgtttcatt atcaccagtt aataaatgag tcttaatctt tttcttttta
      241 agaaaactaa ttaaatgctt tgcatctttt cttaattcgt cttgaattac aatataacca
      301 gaatatttat tattaatgct tacatagata atagagccta cggtttctac ttcttctaat
      361 tttattccgt tttctttaag aaaactagca ctaccaataa ttatactctt atcatctact
      421 attgttttaa ttccttgtcc ctttatttct tcaaacattt taactctttg aagatctagc
      481 ttgtctctat attcacttac aattgactta gcaattgggt gatgtgattt aatttcactt
      541 aaagcaacta attcaaaaaa ctcatcttta ctatagtcaa tagtatgaat tttagaaaca
      601 cgtaaaactc catgtgtcat tgtacctgtt ttatctaaat aaacagtttt aatattttct
      661 aaaacatcta aactattacc acttttaatt aaaatacctt ttctactggc agccccaaca
      721 cttaaaaaat aagcaagagg aacagaaatt acaatcgagc aagggcaaga aacaactaag
      781 aaaattaaag ctcgataacc ccaagtttcc aaactagtag ttgtaaatcc taaaatgctt
      841 acaggtataa tgaaaattaa tactgctaaa agcaaaataa ttggtgtata gtattttgcc
      901 attttactta ctctaagttc tgtttcgctt ttgttttctt ttgcttcttc taaaaaacta
      961 ataatttgat taatctttga attagatgca tcacttgttg ttttaattct caaagttccg
     1021 ctttctaaaa tataaccact aacaacttct tcgcctttaa tagcctgaag ttgttttgat
     1081 tctcctgtaa tcattttcgt atttaagtat ccttcaccat caactataat accatctaga
     1141 agcactttct ctcctgtttt taccaaaaca atactatcaa tctttacatc accaatatcc
     1201 attctaataa cttcatcatt tacaataaca ttaacctctt tttctgtaaa tgcaagtaat
     1261 tcttgaattg attttgtaga gcgattaatt gataaatctt ttaataattc acccacttga
     1321 tatagtaaca taactaaaat actatctgtt ggctcattaa ttataaaaga tccaattgat
     1381 gcaaccatca ttaaaaaatg ctcatttaaa gaaaacttag aaaataaagc tttaaatgct
     1441 cggtatatta tattatagct aataattaag taacttaaaa taaaagtaac tagtttataa
     1501 gtctggtctt taaaaacaaa tgatccaata agaagtaaaa ttgtagctac taaaattcta
     1561 cttatgagta gccaattatc cttaacaaaa tttctctttt tttctaaaac aattgaaccg
     1621 ttagtaatat aatttttaat aatattattt attcttctta caagttgttt ctcattttcc
     1681 ttttctttta actttaattt taaagtctgt gtttcgatta aaatttcact ttcaataata
     1741 ttattatctt ttaaaagatc tcttttaatt tttcttgaac aactaggaca agtaacatta
     1801 cgcaaattaa aactatatga aacatattca tctgttttaa aactatgccc atgcttttgt
     1861 aatacttttt gaatttcaat atcagccttt tttagttttc catcatcttc agaagaaaat
     1921 aaaatcttat tgcctgagat ttttttggaa ctgattttat tattttggag aattaaattt
     1981 atttcatcat tacatactag acaattataa tctttgattt ctatttcttt aactctcatc
     2041 tttttcctct cttacgtgta ttaaggcaag ctcataaagc gtcttaatgt gatgatctgc
     2101 tagataataa attctttttt tcccttgtct tttaaagcga acaatatctt gctcttttaa
     2161 aacacgtaat tgatgactta cagctgattg attcattccc gtaatatttg taagttcagt
     2221 aaccgacaat tcagattcaa ataaggctat gattatagta aaacgagttg aatctcccat
     2281 agtcctaaat aacaaagtta ttttttcttg ttcgtctgga gttataattt tatttttaat
     2341 tttatctaca ttcatattaa tctcctttaa gttattatat tattttctca tatgaatgtc
     2401 aatacatatg aatatacttt catatataaa aacacattag tttatctaat gtgtttattt
     2461 cttatattga tatcctaatt tttcaattgt ttttaaaact aaaatatcca ttgcatctac
     2521 aattatatac tcttttaaat attcttttaa taaagataat tcagtgcaag ctagcacata
     2581 aacaatagac ttatctgcca acattttctt taatctaatt gcaatagctt ccaaatcata
     2641 tccttcttta atttgataaa taaattgatc taaagatttt tgactcatat agtctgcata
     2701 actaatattt aaatcctttg acctataaac attagccaca ttagttcccg atgttgataa
     2761 aacgataatt ttcttatcat tatatttttc tttcaaatag ttaagtgttt cttcaaccat
     2821 attaataaac tttaagcctt ggaagtctac cttatctaca aaataatgag cagtgttaca
     2881 aggcataatt acatattcag cacctaactt tttcaaatca gcaattgctt catttaaata
     2941 aggaacaggg ctatttcccc ctgcaacaat agcttgcgtt ctatcaggaa tgctaggatt
     3001 atttaagatg gcaattttta tatgctctct atcaaactta gcatcagttt ttaaaacaat
     3061 tcttcgaaaa agttctgatg tagctaatgg tcccatacca ccaattattc ctagtttata
     3121 catctttacc ttcctttatt aacaaatctt cgtatgcttc tttaaattct tctaaggcgt
     3181 aaactttctt ttgataacca ttaattgttt tattctcaag taaagaattt agaacggctt
     3241 cttctgttg
//
LOCUS       DAOSGS010000100         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_27743_length_3248_cov_3.943000, whole genome shotgun sequence.
ACCESSION   DAOSGS010000100 DAOSGS010000000
VERSION     DAOSGS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27743_length_3248_cov_3.943000"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..247)
                     /locus_tag="PLQ22_04095"
     tRNA            complement(163..247)
                     /locus_tag="PLQ22_04095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:caa)
     gene            complement(288..1001)
                     /locus_tag="PLQ22_04100"
     CDS             complement(288..1001)
                     /locus_tag="PLQ22_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOH59176.1"
                     /translation="MFLNGILLSLADIDKGLLLEQVLRVIACVIIGGAIGYEREIKAK
                     PAGFVTFILVCVGSCIIAILQENLFLANPEHMDRSRIIAQVVSGIGFLGAGTILYQRG
                     AVKGITTAAMLWLVAALGIMVGSGGTLNYIIAAITVVVVLPTVIVSRRLGEKITTARK
                     VYKIRILFEENCEKELFEFFATSGVTVRKSYFVNKIREGKKELKESYIYLSLPKGMTL
                     NELLEKLSAYNEIYEVSEA"
     gene            complement(1003..3045)
                     /locus_tag="PLQ22_04105"
     CDS             complement(1003..3045)
                     /locus_tag="PLQ22_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH59177.1"
                     /translation="MKSRKEGLKLDYTSKEILARVFKYAWRNKFVLLLSVFLLISFTF
                     LEMVQPLITKQIIDTELTGVQTTWVETMDSSKASYNGKFYEKTDQNTGNIYTIKYSKD
                     LEGYIFIEGSFKEYYIESLDQSTNTINLKKDDLSASSNYQVLGDDITKFYSLSKPLIT
                     RWIIIFGIISVLILIFRLTQRVTYVSASMKLTLDLRKETFDKLNRLPIAYFSTEPSGK
                     TVTKMIFDSEGVRGVYDVMFEIFSASISLIIVYVGLFILDYRLALLTFLAFPIIWIWL
                     TVYRSKVNHYNFMIRESNSILNGRLAEFVNSVGVIQIFNKEEKMSNEYNNMLIENTGY
                     RNRRLVITTFFGNEMLMTIQRIIVAFVLLYFGLRYFDTRTLLIATTISVYIDYIGRLV
                     QPISSVFANLNTLEDSLVSASRVFSFLDEKEDTGLGEITGKEFTGKIEFKNVSFYYEK
                     ENYVLKDVSLKINPGDFVGLVGHTGSGKSTMMSLLERYYDLEEGEILLDGENYLNYSK
                     ADIRENIGLILQDAVIFEGTIKENIAYGIETTDEEVINVLLAIGGDKFVYELKDGIHS
                     KTSYQGENLSIGEKQIIAFARILLKNPKILILDEATANIDTETELLIQNALNVLTENR
                     TTFVVAHRLSTIQHADHIIVLDEGRIVEEGKHQELMALKGKYRDMYEAQIRKQGGN"
     gene            complement(3035..>3248)
                     /locus_tag="PLQ22_04110"
     CDS             complement(3035..>3248)
                     /locus_tag="PLQ22_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH59178.1"
                     /translation="ILESLKKYRANKTNIIIAHRLSAVAEADKIIVLDKGTISEEGTH
                     EELMAKQGWYYTQYKNQELKGEKDEI"
BASE COUNT         1113 a          614 c          364 g         1157 t
ORIGIN      
        1 ttaataataa tttaacgatt taattttata tataaatgat atatatacta ctacttctat
       61 atttatagct gaaatgttct ataaatcatt aatatttgca gtatttatgt ctaaaaaaga
      121 aaaaatagcc gatattttgg tatcgactat acttaactct aatggtgccg ctggccagac
      181 tcgaactggc atgcttttca gcgcttgatt ttgagtcaag tttgtctacc tattccaaca
      241 cagcggcata gctactatta cttttaagta atagtagttt ttttaattta agcttctgaa
      301 acttcgtaaa tttcattata agctgatagt ttttcaagaa gttcatttaa agtcattcct
      361 tttggtaaag ataagtaaat ataagattct tttaattctt tctttccttc tcttatctta
      421 tttacaaaat atgactttct aactgtaact ccactagttg caaagaactc aaatagttct
      481 ttctcacaat tttcctcaaa aaggattcta atcttataaa cctttctagc tgttgtaatc
      541 ttttctccta atcttcttga aacaataaca gttggtaaca caactactac tgtgattgct
      601 gcaataatat agttaagtgt tccaccagaa cctaccataa ttcctagtgc cgcaactaac
      661 cacaacatcg ccgctgtagt aatcccttta actgctcctc tttggtaaag aatagtacca
      721 gcaccaagaa aaccaattcc tgatacaact tgagcgatta ttcttgatct atccatatgt
      781 tctggattag ctaagaataa gttttcttgt aagattgcga taatacaact acctacacaa
      841 actaaaatga aagttacaaa tccagctggt ttagctttaa tttctctttc ataaccgata
      901 gctccaccga ttattacaca agcaataact cttaagactt gttctaataa aagtccttta
      961 tctatatcag ctagactcaa taaaatgcca tttaaaaaca ttttaatttc ctccttgttt
     1021 ccttatttgg gcttcataca tatctctata tttacccttt aaagccatta attcttgatg
     1081 tttcccctct tctacaatcc ttccctcatc taagacaata atatggtctg cgtgttggat
     1141 tgtagataaa cgatgtgcaa ctacaaacgt cgttctattt tctgttaaga catttaaagc
     1201 attttgaatt aaaagctcag tttctgtatc aatattagct gtagcctcat ctaaaatcaa
     1261 aatcttagga ttctttagta aaatcctcgc aaaagcaata atttgctttt ccccaattga
     1321 taagttttca ccttgataac tagtcttact atgaatacca tcttttaact cataaacaaa
     1381 cttatcacca ccgattgcaa gaagtacatt tattacttcc tcatcagtag tttcaatgcc
     1441 ataagcaata ttctctttaa ttgttccttc aaaaataacg gcatcttgta agattaaacc
     1501 aatgttttct ctaatatctg ctttagaata gtttagataa ttctcaccat caagtaagat
     1561 ctctccttct tctaaatcat aatatctttc taaaagactc atcattgtac tcttacctga
     1621 accagtatgt ccaactaacc ctacaaaatc acctggatta atctttaagg atacatcttt
     1681 taaaacatag ttttcctttt catagtaaaa agaaacattt ttaaattcaa tcttacctgt
     1741 aaactcttta ccagttattt cccctaatcc cgtatcttct ttttcatcta gaaagctaaa
     1801 aacacgggaa gcagaaacta gtgagtcttc taaagtatta aggttggcaa agactgaaga
     1861 tattggctga actaatctac caatatagtc aatataaaca gagattgttg ttgcaataag
     1921 tagtgtcctt gtatcaaaat accttaaacc aaagtataaa agtacaaaag ctacaataat
     1981 tctttgaatt gtcattaaca tctcatttcc aaaaaatgtt gtaataacta atcttcgatt
     2041 acgataacca gtattctcaa taagcatatt attatattca ttactcatct tttcttcttt
     2101 attaaagatt tgaataacgc caacactatt tacaaactct gctaaccttc catttaaaat
     2161 tgagttagac tctctaatca taaagttata atgatttact ttacttctat aaactgtaag
     2221 ccaaatccaa attattggaa aagctaagaa agtaagtaaa gctaatctat aatctaaaat
     2281 aaataaacca acataaacta taattaacga aatcgatgca ctaaagatct caaacattac
     2341 atcataaaca cctcttacac cctcagaatc aaagatcatc tttgtaactg tcttacctga
     2401 aggttcagtg ctaaagtagg caattggtaa tctatttaac ttatcaaaag tttcttttct
     2461 taaatctaaa gttaacttca ttgaagcact aacataagta accctttggg ttaatctaaa
     2521 gattaaaatc aaaacagaaa taataccaaa gataataatc catcttgtaa ttaaaggttt
     2581 agataaacta taaaacttag ttatatcatc tcctaaaact tgataattac tacttgcgct
     2641 taaatcatct ttctttaaat taatcgtatt agtactttga tctaagcttt caatataata
     2701 ttctttgaag cttccctcaa taaagatata accttctaaa tccttagaat atttgattgt
     2761 ataaatgttc cccgtatttt ggtctgtttt ttcataaaac ttaccattgt aacttgcttt
     2821 agatgaatcc attgtttcaa cccaagttgt ctgaacacca gttaactcag tatcaataat
     2881 ttgctttgta attaaaggct gaaccatttc taaaaaagta aacgagatta ataaaaacac
     2941 cgataaaagc aaaacaaact tattacgcca agcatattta aatacacgag ctaggatttc
     3001 ttttgatgtg taatctagct tcaaaccttc ttttctagat ttcatctttt tcacccttta
     3061 actcttgatt tttatactga gtataatacc aaccttgctt tgccattaac tcttcatgag
     3121 ttccttcctc actaattgta cctttatcta aaactataat cttatcagct tctgctacag
     3181 ctgatagacg gtgagcaata ataatattag ttttattagc acgatatttt ttaagcgatt
     3241 ctaaaata
//
LOCUS       DAOSGS010000101         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_29261_length_3130_cov_4.787317, whole genome shotgun sequence.
ACCESSION   DAOSGS010000101 DAOSGS010000000
VERSION     DAOSGS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29261_length_3130_cov_4.787317"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PLQ22_04115"
     CDS             <1..1135
                     /locus_tag="PLQ22_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOH59179.1"
                     /translation="TNRYISSNPKKRKGEDLIISIPPKPNFNLRLVADRLPDQVNKYP
                     PTRFMGSKNKLLSEIWSIASQFQFDTAVDLFSGSGIVGYMFKSQGKKVFSNDYMAMSS
                     LFAQAMIENNSITLSETEVSNLLKPNDLVDQFVQLTFKDLYFTDQENMLIDIIRCNSN
                     LINNKYKRAIARSALIRACLKKRPRGIFTYVGHRYDDGRKDLQTSLHDHFLMAIEAIN
                     NSVFNNSQKNKSYLGDALDFKPVENSLIYIDPPYYSPYSDNEYVRRYHFLEGIALDWK
                     GVEIQEHTSTKKFKSYPTPFSSRKGALDAFNMLFKRFKNSILLVSYSSNSLPTQDEIV
                     GLMSNYKNHVEVVPIDYKYSFGNQAHKVGNNKNDVHEYLFVGY"
     gene            1138..2142
                     /locus_tag="PLQ22_04120"
     CDS             1138..2142
                     /locus_tag="PLQ22_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59180.1"
                     /translation="MIKTYNFPQADKIEQVGMVAIAVSKGYLTDERIEKYIKLDSGGR
                     QGRYYRHSAEILGLIINKGNHSELTPIGKEFSQLRSKTAQNEFLAYRIIETPVFRKAL
                     DYIYLHKPNDRRLKLWFKNFYPGSPSTAERRYATFISYLKEVKIVSLKDDLFEVTKFD
                     GALARKEYNPLKTLAGKNISTIIQNEFITSKNKNIQYEVNAQKREQANQIHWKLVVSK
                     SSFLSEIGFPAYGNEHIDLFSRINNKVIIYEMKSMNSKNLLSQIRKGISQLYEYRYIY
                     SKPNAKLCIVGNHNIGKSNSWLLHYLSIDRLIAFEWTDNYHDFYCDKNSKKILSEFAP
                     "
     gene            2218..>3130
                     /locus_tag="PLQ22_04125"
     CDS             2218..>3130
                     /locus_tag="PLQ22_04125"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HOH59181.1"
                     /translation="MLANSPLLGKVKGRENENSFARLLNKNGIVNITRESSDASDLGR
                     KWRSALSQLGFIIPEFISGEHNASDGIGEPFTLSLNGKRLIESDTVPAMQECFLRSLA
                     AYYIPSPIEKEYNFPSFSPLRHTLSIMLNLEDKTGDSWLNFLEMSLIVQLSNSNDNIA
                     LIADQILSFRKERFDSKNKRQFDQNRLDKAAEEHKYAATTFKDYGDTNFRYLRATGLF
                     QSRGRGLTIIPEKHTFIEQLVNDKYVPNTAVEYINGLCNGAKLPTDNKNTAILVLNDL
                     LKTAKANDIQSLLSHTFKIRNPIVTPEE"
BASE COUNT         1131 a          485 c          501 g         1013 t
ORIGIN      
        1 aactaatcgt tatatttcat caaacccgaa aaaaagaaaa ggagaagatt taattatttc
       61 aattccccct aaaccaaatt ttaatctacg tttggtggca gacagactac cagaccaagt
      121 caataaatat cctcccacac gatttatggg atcaaaaaat aaattattat cagaaatatg
      181 gtcgattgca tcacaatttc agtttgatac agcagtagat ttattctctg gttctggtat
      241 agttggatac atgtttaagt ctcaagggaa aaaagttttt agtaacgact atatggctat
      301 gtcttcattg tttgctcaag caatgattga aaataactca attactctat ctgaaaccga
      361 agtaagcaat ttattaaagc ctaatgattt agtagatcag ttcgttcaat taacttttaa
      421 agatttatat ttcaccgatc aagaaaatat gttgattgat attattcgat gtaattctaa
      481 tcttattaat aataagtata aaagagctat cgcaagatca gctttaatac gagcttgcct
      541 taagaagcgg ccaaggggaa tttttacata tgttggacac cggtatgatg atggtcgcaa
      601 agatttacaa acttcgttac atgatcattt tttaatggca attgaagcga taaataattc
      661 agtttttaac aattcccaaa aaaataaatc atatcttggt gatgcattag attttaaacc
      721 tgtagagaac agtttaattt atattgatcc gccgtattat tcaccatatt cagacaacga
      781 gtatgttagg cgttatcatt tcttagaagg cattgcttta gattggaaag gtgttgaaat
      841 tcaagagcac acctcaacaa aaaaattcaa atcttatcca acaccttttt cgtcaagaaa
      901 aggtgcattg gatgcattta atatgctttt taaacgcttt aaaaatagta tactattggt
      961 ttcatactct tcaaatagcc tgccaacaca agatgaaatc gttgggctta tgtcaaatta
     1021 taaaaaccat gttgaagttg tacctattga ttataaatat tctttcggca atcaagctca
     1081 taaagtaggt aataataaaa atgatgtaca cgaatatttg tttgttggat attaacaatg
     1141 ataaagactt ataattttcc acaagctgat aagattgaac aagtaggaat ggtagctatt
     1201 gctgtttcaa agggttattt aaccgatgag cggattgaaa agtatataaa acttgattca
     1261 ggtggtcgtc aaggtcgtta ttacagacat tctgcggaga tcctcggact gattattaat
     1321 aaagggaatc attcggaatt aacacccata ggtaaagaat tttctcaatt aagatcgaaa
     1381 accgcacaaa atgaattttt agcttataga ataattgaaa ctcctgtttt taggaaagct
     1441 cttgattata tttatctaca caaaccaaat gatagaagat taaaactgtg gtttaaaaat
     1501 ttttatcctg gatcaccaag tacagcagaa cgccgttatg caacatttat ttcttatctt
     1561 aaggaagtta aaatagtttc tttaaaagat gatttattcg aagtcactaa gtttgatgga
     1621 gctttagcaa gaaaagaata taatcccctc aaaacactag ccggtaaaaa tattagtaca
     1681 ataattcaaa atgagttcat aacatcaaag aataaaaata ttcaatatga ggttaatgct
     1741 caaaaaagag aacaggcaaa tcaaatacat tggaaattgg ttgtaagtaa atctagcttt
     1801 ttatctgaaa ttggattccc tgcttatggc aatgagcata tcgatttatt ttcacgaatt
     1861 aataataaag tgataattta cgaaatgaaa tctatgaatt caaaaaattt actttcgcaa
     1921 ataaggaaag gtatttctca attatatgaa tacagatata tttattcaaa acctaatgct
     1981 aaattatgta ttgtcggaaa tcacaatata ggtaagtcaa attcgtggct tttacattat
     2041 ctgtctattg atcgtttaat cgcttttgaa tggactgata attatcatga tttttattgt
     2101 gataaaaatt caaaaaaaat tctttctgaa ttcgcgccat aatttatcac catgagacca
     2161 tggcatctag gcaatacaac cgtaaggagc ccttttcgat taaaagatgg tttaaatgtg
     2221 ctcgctaatt cgcctttact tggaaaagta aaaggtcgcg agaatgagaa ttcttttgca
     2281 agattattaa ataaaaatgg tatcgtaaat atcactcgtg aatcttcaga tgcttcggat
     2341 cttggacgta aatggcgttc agcattaagt caacttggtt ttattattcc cgaatttatt
     2401 tcaggagagc ataatgctag tgatggtatt ggtgaaccct ttacattatc tttaaatggc
     2461 aaacgtttaa ttgaatctga cacagtacct gcaatgcagg aatgcttttt acgttcgcta
     2521 gctgcctatt atatcccatc ccctattgaa aaagaatata atttcccatc cttttcgcca
     2581 ttacgtcaca ccttatctat catgcttaat cttgaagata aaactggtga tagttggtta
     2641 aattttctag aaatgagtct cattgttcaa ttatcaaatt caaatgacaa tattgcatta
     2701 atcgcagatc aaatcttatc ttttcgaaaa gaaagatttg attctaaaaa taaacgtcag
     2761 tttgatcaga atcgattaga taaagccgcc gaagaacata aatatgcagc aactacattt
     2821 aaagactatg gtgacacaaa ttttagatat ttaagagcta caggtttatt tcaaagtcga
     2881 ggacgaggcc ttactattat tccagaaaaa catactttca tagaacagct agttaatgac
     2941 aaatatgtcc ctaatactgc agttgaatat attaatggat tatgcaatgg ggcaaaacta
     3001 cctacagata ataaaaatac tgcaatatta gtacttaatg atttattgaa aacagcaaaa
     3061 gcaaatgata tacagtcact actgtcccat acatttaaaa tccgaaatcc aattgtaact
     3121 ccggaggaac
//
LOCUS       DAOSGS010000102         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_29522_length_3111_cov_3.822644, whole genome shotgun sequence.
ACCESSION   DAOSGS010000102 DAOSGS010000000
VERSION     DAOSGS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29522_length_3111_cov_3.822644"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PLQ22_04130"
     CDS             complement(<1..1279)
                     /locus_tag="PLQ22_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOH59182.1"
                     /translation="MYSKDFIKVAAASPKIVLGKPLENVKEMLKILKKLDQDVDVIVF
                     PELAITGYSCGDLFYSDQLYLETQEAIKYLLNNNPFKGVVLFGSYVFQNSVIYNCSFV
                     VQDNELIGVVPKEYLPHSEEFDETRWFGKKLDGISNLEFFKDTDEKDDNFYNPIPFGK
                     MLFKFYNSDIIFGCEVCGDMWAPQSVSEELYLKGADIVFNCSSSPARIYKTKKRELII
                     NNLTYKYSGAYVYASTGASETSSNVIFGSELIISEAGKTLIIDDKLSFDSKVIVSEID
                     ITSLRYKRRKSSYYKQAYMKANNELSSDLSLHEDLVKSADKCKLTREFDKNPFVPKDD
                     LYLDQIISIQTAAVYKRFMHVGAKKLVLGISGGQDSTLALLSLVKICDTYNLPRNTIL
                     GVTMPTSNTKETSYNLALALMRKLGVEVLDLNIE"
     gene            complement(1288..1845)
                     /gene="nadD"
                     /locus_tag="PLQ22_04135"
     CDS             complement(1288..1845)
                     /gene="nadD"
                     /locus_tag="PLQ22_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOH59183.1"
                     /translation="MKIIYGGSFNPPTIAHYKIAKYLLKKFKDSEIIFMPTSSLYHKD
                     ELASNKDRLKMLEIVAEELGERASVSDFEMKQDGYYGTTYTLAHFPESYFLLGADNLV
                     NINKWINFPNIVIQNKFIVIPRGNIDIMAIIEKDDFMSKHKENFTILEDAPKLNVSSS
                     EYRKTKNKKILLTKVNQYIKENNLY"
     gene            complement(2038..2960)
                     /gene="rsmH"
                     /locus_tag="PLQ22_04140"
                     /pseudo
     CDS             complement(2038..2960)
                     /gene="rsmH"
                     /locus_tag="PLQ22_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
BASE COUNT         1009 a          550 c          368 g         1184 t
ORIGIN      
        1 cttcaatatt taaatctaat acttcaacac ctaattttct cattaaagca agtgccaaat
       61 tataacttgt ctcttttgta ttgcttgttg gcattgtaac acctaaaatc gtatttcttg
      121 gtaaattata agtatcacat attttgacta atgataataa tgctaaggta gaatcttgtc
      181 caccactaat tcctaaaaca agttttttag caccaacatg cataaatctt ttataaactg
      241 ctgctgtttg aatactaatg atttgatcta aatataaatc atctttaggt acaaatggat
      301 tcttatcgaa ttctcttgta agcttacatt tatctgcgct ctttactaaa tcttcatgaa
      361 gagaaagatc tgaagataat tcattatttg ctttcatata agcttgttta taatatgaac
      421 tctttcttct tttatagcga agagatgtaa tatcaatttc actaacaatt acttttgaat
      481 caaatgatag cttatcatca attattaaag tctttcctgc ttcagaaata attaactctg
      541 aaccgaaaat aacattactt gatgtttcac tagccccagt tgaagcataa acatatgctc
      601 cactatattt ataagttaag ttattaataa ttaattctct ttttttagtt ttatatattc
      661 ttgctggtga agatgaacaa ttaaaaacaa tgtccgcacc ttttaaatat aactcttcac
      721 taacagactg tggtgcccac atgtccccac aaacttcgca accaaaaata atatcgctat
      781 tataaaactt aaacaacatt ttaccaaaag gaataggatt ataaaaatta tcatcttttt
      841 catcagtatc tttaaaaaac tctaaattac taattccatc taacttttta ccaaaccacc
      901 tagtttcatc aaactcttct gaatggggta agtattcttt tggtactaca ccaattaatt
      961 cattatcttg aacaacaaaa ctacaattgt aaataacact attttggaaa acataagaac
     1021 caaaaagcac aacaccttta aaggggttgt tatttagtaa atatttaatt gcttcttgtg
     1081 tttctaaata tagttgatca ctataaaaca agtccccaca agaatagcca gtaatagcaa
     1141 gttctggaaa aacaataaca tctacatctt gatctaattt ctttaagatc ttaagcattt
     1201 ccttaacatt ttctaatggt tttcctaaaa caatttttgg acttgcagct gcaactttaa
     1261 tgaaatcttt actgtacatc tttcctccta gtacaaattg ttttctttaa tatattgatt
     1321 tacctttgta agcaatattt ttttattttt agttttacga tattcagaac ttgaaacatt
     1381 taatttaggt gcatcttcta aaatcgtaaa gttttcttta tgtttactca taaaatcatc
     1441 tttttcgata atcgccataa tatcaatatt accacgtgga ataacgataa atttgttttg
     1501 aataacaata tttgggaagt taatccattt atttatatta actaagttat cagctccaag
     1561 taaaaaatat gactctggaa agtgagcaag tgtgtatgtt gtaccataat aaccatcttg
     1621 tttcatttca aaatcagaaa ctgatgctct ttcccctaat tcttctgcca caatctctag
     1681 cattttcaaa cgatctttat ttgaagctaa ttcatcttta tgataaagtg aactggttgg
     1741 cataaatatt atttctgaat ctttaaattt ttttagtaaa tattttgcaa ttttataatg
     1801 tgcaatagtt ggcggattaa atgaaccacc ataaataatc ttcatatttt tacctcttac
     1861 atattatacc acatttccaa agtaaaagac aactttttat ccaaaagttg tctttttaca
     1921 ataaataagt ataaaataag gaattatatg tgtttatgat atatttcata tcaactataa
     1981 ttatcttggt aataccggtt ttggtaaagg tcttaagttt gtcattattt gtacctccta
     2041 tagtttttga atgattctta attttgccga gcgtgaacgt atattagttt ctagctcttc
     2101 cgggcttgaa gttatacctt tttattaact aaagcaaact ctttattaat cttataatct
     2161 tcgattggaa gatcttttgg catattatct tctgtaactt ctttaaagac ctctttacag
     2221 atgcggtctt ctaaagagtg gaaggttatc acgcaacatc tcccattcgt atcaagtaaa
     2281 tctaaagcat cccttaaaga tttctcaaac acatttaact catcgttaac ggcaatccta
     2341 attgcctgaa aagttttacg tgcagggtgt gtagcttttc ttcggtaatg agctggaact
     2401 gcttgtgtaa caattctaac taattccgtt gtcgtctcaa ttggttttac ttttctaaaa
     2461 taatctattt tctttgcaat actcgttgca tatttttctt ctccatattc gaagataatt
     2521 cttcttaact cttcgactgg atactcattt actacatcat aagctgttaa agcttgattt
     2581 tgattcattc tcatatcaag cctagcttct tcatgataac taaatccacg atctctatca
     2641 tcaagttgaa atgaagaaac tcctaagtca tataaaattc catcaacttt ataaactcct
     2701 aaactattta gttgttcttt aatatttaca aagttatcag ggattattgt aaagttatta
     2761 ctaatcttag caagtctttc tcttccttca ttaattgcat aatcgtcttg ttcaaaacag
     2821 taaagtttac catttgtaag tttttctaaa atgatgctag catggccagc tccacccatc
     2881 gtacaatcta cgtatattcc atcttcctta acagttaggc ctgtcataac ttcattaagt
     2941 aatactggaa tatggatcat ttttgtttta atcattgtgc ttggtctttg tctaatcatc
     3001 aaaatcaatc ctttcactga tttcatcaag tgtagcttga cgttcttgat aatattctaa
     3061 ccaggccttt ttatcccaaa tttctattct ttcaccaaca ccgataatta c
//
LOCUS       DAOSGS010000103         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_29652_length_3101_cov_5.297439, whole genome shotgun sequence.
ACCESSION   DAOSGS010000103 DAOSGS010000000
VERSION     DAOSGS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29652_length_3101_cov_5.297439"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLQ22_04145"
     CDS             <1..1012
                     /locus_tag="PLQ22_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH59184.1"
                     /translation="GANIKDLRTGELASFVKPYHIVEKEGIKIGIIGLIGLGLESDIS
                     RNFVENFQFTSELEAIKLYAPILRGEHECEIVVVSVHNDTSPINESIANLSGNQRVDL
                     VFNGHTHYYYAAEQRRSNDVPLAIVQSGSSGRYVGKIQVVYDRDNKKVTDISAENIKT
                     ATFSKEDQKILDLYQNYQDVIDISEEVLGQAKVDIYREEATTWAVNVLQKTAGTDFAI
                     INTGGIRRDAFPIRANTDVLYDNIFRIMPFENTIIKTNLTGSQIKGLRGSNPMAIRDG
                     LNYDNLVDDELYTIATIDYLFYKDYRLESGANPDFTGDLFRDYLVAAVKQSVLENGYW
                     IL"
     gene            1040..2311
                     /locus_tag="PLQ22_04150"
     CDS             1040..2311
                     /locus_tag="PLQ22_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH59185.1"
                     /translation="MGYTKLNIYNISDLHGVILESRRSAGLLRTASYLKEEIQKNPNS
                     IIIAGGDIYSGTYFSKLTQGKVINLIFNSLNLTCLGLGNHELALADKLKTLEKEASFP
                     FVLANVYNKDNELMFAPYTVKEIGNLKVGIIATLGEKQKDDLTSEIASQIVVTDELET
                     IREYSYRLRRKEKCDLIIVSSHSSDDSLKQQLCYFNNKEKVDVLITSHTHEVFAYTFT
                     KNKHKLINLSSGGYGEYLGVIELVLKRRKIVDSKVNNLNTLGLPHNIYHQYQEIISDY
                     MRTNKDLWKVVGKVKNNYFKEEFLRQVTNYILNKEEADFVLLNYGSIRSIAFPLIEGS
                     EITESDLYEINPFSNYLYKIEIDRNTLNNYLNQYYSATYLHSKDILRKEKYNVVINDF
                     LKDKYFAFSLKEKKLVKVTDYIKEMLAKELL"
     gene            2308..2721
                     /locus_tag="PLQ22_04155"
     CDS             2308..2721
                     /locus_tag="PLQ22_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59186.1"
                     /translation="MRLENFYILIGETIEYCQRIEYDLKMIYAYMEDGSFSDNLKKVE
                     VLPLGEIIYLIRERDKEQKLFKKADYDILFTITKRRNHIVHQCFKNYNYALTQEEQER
                     KFEIEYKNLEAFHGRLTTLWKAIENVRYNFLSKKL"
     gene            2771..>3101
                     /locus_tag="PLQ22_04160"
     CDS             2771..>3101
                     /locus_tag="PLQ22_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH59187.1"
                     /translation="MRKIKLIVDSASDLGKEYYERLNIDVVPIYVILGETTYQDYYEI
                     NTEQIYAFTEETGIYPKTAAASPALVYDVFKKYYDEGYDILYIGIGSKISATFSVAQA
                     AAKEIDPK"
BASE COUNT         1157 a          307 c          569 g         1068 t
ORIGIN      
        1 tggtgctaat attaaagact taagaactgg agagcttgca agttttgtaa agccatatca
       61 tattgtagag aaggaaggaa taaaaattgg aattattggt ttaattggtt taggattaga
      121 atctgatatt tcgcgaaatt ttgtagagaa ctttcagttt acttctgagt tagaagcaat
      181 taaattgtat gcaccaatat taagaggaga gcatgaatgt gagattgttg tagtttctgt
      241 tcacaatgat actagtccaa ttaatgaatc aattgctaat ttaagtggaa atcaaagagt
      301 tgatttagtc tttaatggtc atacgcatta ttattatgca gctgagcaaa gaagaagtaa
      361 tgatgttcca ctagcaattg ttcaatcagg atctagtggt aggtatgttg ggaagattca
      421 agttgtttat gatagggata ataagaaggt tactgatata agtgcagaga atatcaaaac
      481 agctactttt tctaaagaag atcaaaagat tttagatttg tatcaaaact atcaagatgt
      541 aatcgatata tcagaagaag tgttaggcca agcaaaagta gatatttacc gtgaagaagc
      601 tactacttgg gcagttaatg tcttacaaaa aactgctggt actgatttcg cgataatcaa
      661 tacaggtggt attagaagag atgcttttcc aattagagct aatactgatg tgctttatga
      721 taatatcttt aggatcatgc cgtttgaaaa tacgattatt aagacgaatt taactggaag
      781 ccaaattaaa ggacttagag gatctaatcc aatggctatt agagatggtt taaattatga
      841 taatttagta gatgatgaac tatatacaat agctacaata gactatttgt tttataaaga
      901 ttatcgctta gaaagtggag caaatcctga ttttactggg gatttattta gagattattt
      961 ggtagccgct gtaaagcaaa gtgttttaga aaacggttat tggatattat aatattttaa
     1021 aaataaaagg aggttagcga tgggatatac aaaattaaat atctataaca tcagtgattt
     1081 acatggtgtt attttagaat caagaagatc agctggcctt ctaagaacgg ctagttattt
     1141 aaaagaagaa attcaaaaga atcctaattc aattattatt gcaggtgggg atatttattc
     1201 tggtacttat ttttctaaat taacgcaagg taaagtaatt aatttaattt ttaattcttt
     1261 aaatcttact tgtttaggtt tagggaatca tgaacttgct ttagctgata aacttaaaac
     1321 tttagaaaaa gaagcttcat ttccttttgt tttagcaaat gtatataata aggataacga
     1381 gttaatgttt gctccttata cggttaaaga aatagggaat ttaaaagtag gaattattgc
     1441 tactttaggt gaaaaacaga aagatgatct tacaagtgaa atagcaagcc aaattgttgt
     1501 tactgatgag ttagagacaa ttagagaata tagttatcgt ttaagaagaa aagaaaaatg
     1561 tgatttaatt attgtttcat ctcatagtag tgatgatagt ttgaaacaac agctttgtta
     1621 ttttaataat aaagaaaaag ttgatgtttt aataacttct catactcatg aagtatttgc
     1681 ttataccttt acaaaaaata agcataaatt aattaattta tcttcaggtg gttatggaga
     1741 atatttagga gtaattgagc ttgttttaaa aagaaggaaa attgttgata gcaaggtaaa
     1801 taatcttaat actttaggac ttcctcacaa tatatatcat caatatcaag agataatttc
     1861 agattatatg agaactaata aagatttatg gaaggttgtt gggaaagtta aaaacaatta
     1921 ttttaaagaa gagtttttaa ggcaagttac caattatata ttgaataaag aagaagctga
     1981 ttttgtttta ttaaactatg gttcaattag aagtatagct tttcctttaa ttgaaggtag
     2041 tgaaattact gaatctgatt tgtatgaaat taatcctttt tctaactatt tatataagat
     2101 agaaatagat cgtaatactt taaataatta tttgaatcaa tactattctg cgacttattt
     2161 gcattctaag gatattttaa ggaaagaaaa gtataatgtt gtcataaatg attttcttaa
     2221 agataaatat tttgcttttt ctttaaaaga aaagaaatta gttaaagtaa ctgattatat
     2281 aaaagagatg ttagcaaagg aattactatg aggttagaaa acttttatat tttaattggt
     2341 gaaactattg aatattgtca gcgcattgag tatgatctga aaatgattta tgcttatatg
     2401 gaagatggga gttttagcga caatttaaag aaggtagaag ttttaccttt aggagaaatt
     2461 atttatttga ttagagagag agataaggaa caaaaacttt ttaaaaaggc agattatgat
     2521 attttattca caattaccaa aagaagaaat catattgtgc accaatgttt taagaattac
     2581 aattatgctt taactcaaga agagcaggag agaaaatttg agatagagta taaaaattta
     2641 gaagcttttc atggaaggct aactacttta tggaaagcta ttgaaaatgt taggtataac
     2701 tttttaagca aaaaactttg atattcctct aattaaagtt ataattaaac taggcaaagg
     2761 aagtaaaaaa atgaggaaaa ttaaattaat tgtagatagt gcaagtgatt taggtaaaga
     2821 gtattatgag agattaaata ttgatgttgt tccgatttat gtaattttag gtgaaactac
     2881 ttatcaagat tattatgaaa tcaatacaga gcaaatttat gcttttactg aagagactgg
     2941 catttatcct aaaactgctg cagcatcgcc tgctttagtt tatgatgttt ttaagaagta
     3001 ttatgatgaa ggatacgata ttctttatat tggtattggt agtaaaattt ccgctacgtt
     3061 ttctgtagct caagcagcag ctaaagaaat tgatcctaag g
//
LOCUS       DAOSGS010000104         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_29677_length_3099_cov_3.981603, whole genome shotgun sequence.
ACCESSION   DAOSGS010000104 DAOSGS010000000
VERSION     DAOSGS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29677_length_3099_cov_3.981603"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLQ22_04165"
     CDS             complement(<1..472)
                     /locus_tag="PLQ22_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59188.1"
                     /translation="MIIKKANPCGFCSGVKGAITKVINLIEDQNIEKPLYILGYLVHN
                     RFINDALKTKKIKVITEEDIFQIKKGTVILTAHGTPKKTIDLIKSQKLNLVDATCPKV
                     KHIHKQIEKYIKDDYQVLFLGVKNHPETKAITSNYNVSLITLDDLSFSKEKSKLV"
     gene            553..1404
                     /locus_tag="PLQ22_04170"
     CDS             553..1404
                     /locus_tag="PLQ22_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59189.1"
                     /translation="MVKIRNKTLSVEIALKGAELKKLASSISYIHDSNPLYWNKSAPF
                     LFPIIGKLRDGKTIIDGKEYEIEKHGFLKGMVFDIVSQEEDEVVLRSKYSEETLLSYP
                     FKYEVDIKFKLKGMSLLVDIKIKNVDDKKMAFNFGWHPAFKISKIEDYRLVFEKEEDI
                     KSPKVHSNSTLDFNTIVYANKTKELKLKRELFNIDTILIKDVKSSYVYLLNKENKGIK
                     LSFPDFKTLAIWTPFDKDAPFICLEPWYGNNDHFDTNYDFYTKDDLIILKPGKCKKFK
                     YQIDLIE"
     gene            1451..2008
                     /gene="efp"
                     /locus_tag="PLQ22_04175"
     CDS             1451..2008
                     /gene="efp"
                     /locus_tag="PLQ22_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH59190.1"
                     /translation="MVSSNNFKTGMTIEYKGDIFQILEFQHVKPGKGAAFVRSRLKNL
                     RTGAIIDYTFKSDERMATAQIDKKDMQYSYSSGEYLVFMDTETWEQLEIPQERMTNEL
                     YYIKEGDKVTIIMFQHEILGIQLPDKVTLEVVQTPPGVRGDTAANATKEATLETGLVI
                     QVPLFVNEGDKVIVSTVDGKYSSRA"
     gene            2012..2782
                     /locus_tag="PLQ22_04180"
     CDS             2012..2782
                     /locus_tag="PLQ22_04180"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOH59191.1"
                     /translation="MVLAIDIGNTNIVLGLFEKDELKAKFRIQTSKLETIDGYGIRCY
                     NLINYAGYKVEDIEGVVISSVVPELDYTFENLVAKFFKQKALFVIPGTKTGLKIKLEN
                     PKQLGSDLIAAAVAGIEEYTVPLIMIDLGTAITIAVINDKKEYLGGVIYPGINTAFAS
                     LTQAAAKLESARMEKVDNVIGRDTVSSIQSGMLFGTASMLDGMIRKIKASYPNALVIL
                     SGGHGSLIKEYLEEKVILDDDVVLKGLNIIYKKNKKAS"
     gene            2869..>3099
                     /locus_tag="PLQ22_04185"
     CDS             2869..>3099
                     /locus_tag="PLQ22_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59192.1"
                     /translation="MDILLEANLSTEALIIILVCTIPVAFLIIMAIINTVRLTKKRKT
                     IREAAISEQPLDEEQQKIFYEVYGGLENIKSIR"
BASE COUNT         1190 a          346 c          521 g         1042 t
ORIGIN      
        1 aaactaattt acttttttct tttgaaaaag ataaatcatc taaagtaatt aaacttacat
       61 tgtaattact tgtaattgct tttgtttccg gatgattttt aactcctaaa aataacactt
      121 gataatcatc tttaatatat ttttctattt gcttatggat gtgtttaact ttagggcaag
      181 ttgcatcaac aagatttaat ttttgagatt taattaaatc aattgttttt tttggtgtac
      241 catgtgcagt taagataact gtaccttttt taatttggaa gatatcttct tcggtaatta
      301 cttttatttt ttttgttttt aaagcatcat taataaaacg gttatgaact agatatccta
      361 aaatataaag aggtttttct atattttgat cttctattaa attaattact tttgtaattg
      421 ccccttttac tccagagcaa aacccacaag ggttagcttt tttaataatc atacttttca
      481 ccttaaagtt attatatcat aaatcactat gattttcttt taaaatttgg taaaatagat
      541 aaaggtgata atatggttaa aataagaaat aaaactttaa gtgttgaaat tgctttaaaa
      601 ggtgcagaac ttaaaaaact agcgagttcg atatcttaca ttcacgattc taatcctttg
      661 tattggaata agtcagctcc atttttgttt cctattattg gaaagttaag ggatgggaag
      721 actataattg atggtaaaga atatgagatt gaaaaacacg gctttttaaa aggaatggta
      781 tttgatattg taagccaaga agaagatgaa gtagttttaa gaagtaaata tagcgaagaa
      841 actcttttaa gttatccttt taaatatgaa gtagatatca aatttaaatt aaaaggaatg
      901 tctttattag ttgatattaa aattaagaat gtagatgata aaaaaatggc atttaatttt
      961 ggttggcatc cagcatttaa aatatctaaa atagaagatt atcgtttagt ttttgaaaag
     1021 gaagaagata ttaagagtcc aaaagttcat agtaatagta ctttagattt taatactatc
     1081 gtttatgcaa ataaaactaa agaattaaaa ctaaaaagag aattatttaa tattgataca
     1141 atcttaatta aagatgttaa gtcaagttat gtatatttac taaacaaaga aaacaaggga
     1201 ataaagctaa gttttccaga ttttaaaaca cttgcgattt ggacaccttt tgataaagat
     1261 gcaccattta tttgtttaga accttggtat ggaaataatg atcattttga tactaactat
     1321 gatttctata caaaagatga tttgattatt ttaaaaccag gaaaatgtaa aaaatttaag
     1381 tatcaaatag acttgataga atagacaagt acgatgaaat gtagtataat aataaaaatg
     1441 ggaggatatt atggtatcaa gcaataattt taaaacagga atgacaatag aatacaaagg
     1501 agatattttc caaatattag agttccaaca cgttaaacca ggaaagggtg cagcttttgt
     1561 aaggagccgt ttgaagaatt taagaacagg agccattatt gattatacat ttaaaagtga
     1621 tgaaagaatg gctacagctc aaattgataa aaaagatatg cagtattcat attcatctgg
     1681 tgaatattta gtatttatgg atactgaaac ttgggagcaa ttagaaattc ctcaagagag
     1741 aatgactaat gaactatact atatcaaaga aggtgataaa gttacgatca ttatgttcca
     1801 acatgaaatt ttggggattc aattaccaga taaagttaca ctagaagttg ttcaaactcc
     1861 accaggagtt cgtggtgata cagctgctaa tgctacaaaa gaagcaacac tagagacagg
     1921 tttagttatt caagttcctt tatttgtaaa tgaaggcgat aaagttatag ttagtacggt
     1981 tgacggaaaa tatagctcaa gggcttaaag tatggtacta gcaattgata taggaaatac
     2041 gaatatcgtt ttaggacttt ttgaaaaaga cgagttgaaa gctaaattta gaattcaaac
     2101 ttcaaagcta gaaactatag atggttatgg tattagatgt tataacttaa ttaactatgc
     2161 aggttataaa gtagaagata ttgaaggagt tgttatttct tcagttgtac cagaactaga
     2221 ttatactttt gaaaatttag tagcaaagtt tttcaaacaa aaagctttat ttgtaattcc
     2281 aggaacaaaa acaggattaa agataaaatt agaaaatcca aaacaattag gatctgattt
     2341 aattgcagct gcagtagcag gtattgaaga atataccgta cctttaatta tgatagactt
     2401 aggcactgct attactatcg cagttattaa tgacaaaaaa gagtatttag gtggagttat
     2461 ctatccaggt attaatactg cttttgctag cctaactcaa gcagcagcta agttagaatc
     2521 agcaagaatg gaaaaagttg ataatgttat tggaagagat actgtttctt caatccaaag
     2581 tggaatgcta tttggtacag catcaatgct tgacggaatg attagaaaga ttaaagcaag
     2641 ttatcctaat gctttagtta tcttaagtgg gggacatggc tctttaatca aagagtatct
     2701 agaagaaaaa gtaattttag atgatgatgt tgttttaaaa ggattaaata ttatctataa
     2761 aaaaaataaa aaggcctctt aatttaagag gcttttgaat ataggaagaa aaataaacaa
     2821 tacaaaaata atttgatata tggtataata tgaaaaaagg aggattatat ggatatttta
     2881 ttagaagcaa atcttagtac tgaggcctta attatcattt tagtttgcac tattccagtt
     2941 gcatttttaa taattatggc aattatcaat acagttcgcc taactaaaaa aagaaaaacc
     3001 ataagagaag cggcaattag cgaacaacca cttgatgaag aacagcaaaa aatcttttat
     3061 gaagtttatg gcggtttaga aaatattaaa agtattcgt
//
LOCUS       DAOSGS010000105         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_29719_length_3096_cov_3.266360, whole genome shotgun sequence.
ACCESSION   DAOSGS010000105 DAOSGS010000000
VERSION     DAOSGS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29719_length_3096_cov_3.266360"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2189
                     /gene="mutS"
                     /locus_tag="PLQ22_04190"
     CDS             <1..2189
                     /gene="mutS"
                     /locus_tag="PLQ22_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH59193.1"
                     /translation="VISEDDNNFLSTVSREKNRFIFAYSDLSTGENYITDIPLNEELL
                     LQEILNLKTKEIVISSSFDLSVFSPLEKVTSITYSIYDEDSDVSYLKKLILDLDKEEQ
                     KCANRLLNYILKTQKKVLIHMQEFTKYDIRGFLKIDFTSRRNLELLETIRFQNKKNTL
                     FYTLNKTKTAMGARFLKRNIVFPLIDKAKIEARYDIIDALHKAFIKADDIRTILNDIY
                     DLERIVGRIAYDNANPKDFLQLKQSLKDLPALQGLLKEIGILKYFPNLEKIDDFVFIF
                     KTIDDAINEDAPYTIKEGGIFKDSYNEELDELRHLNDKSQDYLLKLEQRERKRTNIKN
                     LKVGYNKVFGYYIEISKGNIPLVKDEFGYIRKQTLVNSERYITEELKEKEAMILRAEE
                     KMMALEYNMFVSMRDLFKEYTYSLQALSKDLALLDMMQSLARVAKENNYVRPKFQKEG
                     IKIITGRHPVLEQAEDNFIPNDLVMDEEKIMLITGPNMSGKSTYMRQIALIAIMAQIG
                     SFVSAKEAILPLFDAIYTRIGASDDIISGESTFMVEMLEVNKALQNATSNSLIILDEI
                     GRGTATYDGMSLAQSIIEYIHNNINAYTLFSTHYHELTALDQSLKNLKNIHVSIKERL
                     GEVIFMHKVLPGAVDKSYGINVAKLAKLPLEIILRATDLLHKYEAQAVDSEKFSLANY
                     QAPLIYDSKTELEKNILEKLETIDLNKTSPLDALNKLSELQKKLKK"
     gene            2201..3070
                     /gene="mutL"
                     /locus_tag="PLQ22_04195"
     CDS             2201..3070
                     /gene="mutL"
                     /locus_tag="PLQ22_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH59194.1"
                     /translation="MIKQLDKHLINLIKAGEVIEHCYSVVKELVENAIDAGAKNITIT
                     LENSGLKEIKVSDDGSGMSEQEAKLAILPHHTSKILEEADLYHIDTLGFRGEALPSII
                     AVSSFKMKTSDNGVQGLLLSLKGGELVSEALIAQKKGTEITVRSLFYNTPVRLQNLKN
                     EYVELSYIADFVMKIAFARPDIAFKLINNDKLLLQTFGSGNLLEVVNAIYGSEVAKNM
                     LEIKGSSNLYQVSGYISNLNITRSSKGNMTFIVNGRSVKNNKLFNTVLDAYKGYLMIG
                     KYPYVIINITSSL"
BASE COUNT         1193 a          337 c          531 g         1035 t
ORIGIN      
        1 gtgtaatttc agaagatgat aacaacttct tatcaactgt ttcaagagaa aagaatcgtt
       61 ttatttttgc ttattctgat ctatctactg gagaaaacta tataactgat attcctttaa
      121 atgaagaatt gttacttcaa gaaattttaa atctaaagac taaagaaata gttatttcaa
      181 gtagttttga tttaagtgta ttttctcctt tagagaaagt tacaagtatt acttattcta
      241 tttatgatga agatagcgat gtttcttatt taaagaagtt aattttagat ttagataaag
      301 aagaacaaaa atgtgctaat agattattaa attacatttt aaaaactcag aaaaaagtat
      361 taattcatat gcaagagttt acgaaatatg atattagagg ttttctaaaa attgatttta
      421 cttcaagacg taatttagag ttattagaga caataagatt tcaaaacaaa aagaatactt
      481 tattttatac tttaaataag acaaaaacag ctatgggcgc aagattctta aaaaggaata
      541 ttgtttttcc tttaatagat aaggcaaaaa ttgaagcaag gtatgatatt attgatgctt
      601 tacataaagc atttattaaa gctgatgata taagaactat tctaaatgat atttatgatt
      661 tagagaggat tgttgggagg attgcttatg ataatgctaa tcctaaagat ttcttacagt
      721 taaagcaatc tttaaaagat ctaccagctt tacaagggtt attaaaagaa attgggattt
      781 taaaatattt tcctaattta gaaaagattg atgactttgt ttttattttc aaaacaattg
      841 atgatgctat taatgaagat gctccttata ctattaaaga agggggaatc tttaaagata
      901 gttataatga agaacttgat gaattaagac atttaaacga taaaagccaa gattatctat
      961 taaaactaga acaaagagaa agaaaaagaa cgaatattaa aaacttaaaa gttggttata
     1021 ataaagtctt tggttattat attgaaatta gtaaaggtaa tattccttta gtaaaagacg
     1081 agtttggtta tatcagaaaa caaactttag ttaactcaga gcgttatatt accgaagagt
     1141 taaaagaaaa agaagcaatg attcttcgtg ctgaagaaaa gatgatggct ttagaatata
     1201 atatgtttgt aagtatgagg gatttattta aagaatatac ttatagctta caagctttaa
     1261 gtaaagatct agctttactt gatatgatgc agtcacttgc tagagttgcg aaagaaaata
     1321 actatgtaag acctaagttt caaaaagaag gaattaagat tattacaggt cgtcaccctg
     1381 ttttagaaca agcagaggat aatttcattc ctaatgattt agtaatggat gaagaaaaga
     1441 ttatgttaat tactggaccg aatatgagtg gtaaatctac ttatatgagg caaatagcac
     1501 taatagcaat catggcgcaa attggctctt ttgttagtgc caaagaagca atattgccat
     1561 tatttgatgc aatctatact aggataggtg ctagtgatga tattatttct ggtgagtcaa
     1621 catttatggt tgagatgtta gaagtaaata aagcattaca aaatgcaaca tctaattctt
     1681 taatcatctt agatgagatt ggaagaggaa cagcaactta tgatggtatg agtttagcgc
     1741 aaagtattat tgagtatatt cataacaata ttaatgctta tactttattt tcaacgcact
     1801 atcatgagtt aacggcttta gatcaaagtt taaagaactt aaagaatatt catgtaagta
     1861 ttaaagagcg tttaggtgag gttatcttta tgcataaagt attacctggt gcagtagaca
     1921 aaagttatgg aattaatgta gctaaactag ccaaacttcc attagagata atcttaagag
     1981 caactgattt gttacataaa tatgaagctc aagcagttga tagtgaaaag ttttcactag
     2041 caaactatca agcaccatta atttatgatt caaagactga actggagaaa aatattttag
     2101 aaaaacttga aacaatcgat ttaaataaaa catcaccact tgatgcttta aataaattat
     2161 ccgaattaca aaaaaagcta aagaaataaa ggagtattaa atgatcaaac aactggataa
     2221 acatctaatc aatttaataa aagctggaga agttattgag cactgttatt cagttgtaaa
     2281 ggaacttgta gagaatgcaa ttgatgcagg ggctaaaaat atcacgatta ctttagaaaa
     2341 ctctggttta aaagaaatca aagtttctga tgatggttcg gggatgagtg agcaagaagc
     2401 aaaactggct attttaccac accatacaag taagatttta gaagaagctg atttatatca
     2461 tattgatact ttaggattta ggggtgaagc tttaccttca attattgctg tttcatcttt
     2521 taagatgaag acatcagata atggggttca aggactattg ttatctttaa aaggtggaga
     2581 gttagtaagt gaagcattga ttgctcaaaa aaaaggtaca gaaattactg ttaggagctt
     2641 gttttataat actcctgtaa gattacaaaa tcttaaaaat gaatatgtag agttaagtta
     2701 tatcgcagac tttgtgatga agattgcttt tgcaagacca gatattgctt ttaagttaat
     2761 taataacgat aaacttttac ttcaaacttt tggaagtgga aatttacttg aagtagttaa
     2821 cgctatttat ggctcagaag ttgctaagaa tatgcttgag attaaaggta gtagtaattt
     2881 ataccaagta tctggctata ttagtaactt aaatataact agatcaagta aaggtaatat
     2941 gacttttatt gtaaacggaa gaagtgttaa gaataacaaa ttatttaata ctgtcttaga
     3001 tgcttataaa ggttacttaa tgattggaaa atatccttat gtaattatca atattacttc
     3061 aagtttatag tgcatattat aattaatatt attatt
//
LOCUS       DAOSGS010000106         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_30006_length_3075_cov_4.409272, whole genome shotgun sequence.
ACCESSION   DAOSGS010000106 DAOSGS010000000
VERSION     DAOSGS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30006_length_3075_cov_4.409272"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLQ22_04200"
     CDS             <1..685
                     /locus_tag="PLQ22_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59195.1"
                     /translation="STLFIIENALIQICLGAFYILDGLILKEIISSYDYTVYTSLAYI
                     YKFPLFLSISISIILLGQNVVEISDTNATQEYKKDNLISEISNKDNTQITKRSDKIKR
                     TFFISLLLIFASFGSLIVIDTLFPGLILDILGYGQYYSRYLSLIMGFAWMANTTLYFI
                     FVYFLKAYKDSYIFKMLIIFYAVSLIASIILTGNDLNKIIFYVLIIPIIYIIITSITG
                     YFVKKKRNQ"
     gene            725..1489
                     /locus_tag="PLQ22_04205"
     CDS             725..1489
                     /locus_tag="PLQ22_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH59196.1"
                     /translation="MRNYFELLDQFISTDFKLRYKNSYLGILWIILKPLASFAVIYII
                     WTNLFPQSPDFKMSLLLGIMIMNLFSESIMMGMTSLSTKAGIILKVRFPREVSVFSAV
                     TISLFDFAINMIIFTAFAIWNPIDISFIGIILFILSIFSIYFITTGISLFLSIMFIRL
                     RDLNNLVSVVLQLFFWMTPVYYKIEMLPENLQKIISLNPMTHIVTLARRGLLGASNIA
                     TDDFWQIGIVFVLSLIVFGIGVLFFKKRVYKIAEYF"
     gene            1489..2226
                     /locus_tag="PLQ22_04210"
     CDS             1489..2226
                     /locus_tag="PLQ22_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH59197.1"
                     /translation="MEQKPVIEVKDVSKSFYLPHERHDTLVEYLTNPIRLFRRNGEMY
                     QVLKDIDLKVYPGEFLGIMGRNGCGKSTLLKIIAGIYAPTTGKIIINGSVAPFLELGV
                     GFNQELSGRENVFLNGIILGMNKKKIRQKYDEIVQFAELEKFMEMPLKNYSSGMKVRL
                     AFSIAIMTDADIYILDEVLSVGDIAFQKKCDGVFAKFKKEKKTVILVTHSVNAVKEMC
                     TRAVFLKDGIMHDYHDIDKVVEAYING"
     gene            2219..>3075
                     /locus_tag="PLQ22_04215"
     CDS             2219..>3075
                     /locus_tag="PLQ22_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59198.1"
                     /translation="MASKLTLKVKKFKEIIRFESIEFLKILGIKKILSSKVEADRSAV
                     SEINAESIDFSRRAIIVLPNITWGYRTQRPQHIFSRLAKKGFNIFWVSQFTSDKETVG
                     KIDENIYQLNLKTLNKGKLLRDFHIDDVNAQEAYQSLKRLIGGKLKNDSILFTLHPAW
                     YYLTQKFKQVIKIYDLMDLYEGFTDSKTDLVEKEKELISQADLVFATADNLLKNAKDL
                     NPNSFLIRNGADYDNFKEISPSGVLDCISKKKIIGYFGSMSDWLDVKSIEYLAKNNPD
                     LNLVFIGKI"
BASE COUNT         1099 a          411 c          503 g         1062 t
ORIGIN      
        1 ctcaacatta tttattattg aaaatgcttt aatacagata tgcttaggag cattttatat
       61 actcgatgga ttgatactaa aagagattat aagttcttat gattatactg tttacacctc
      121 attagcttat atctacaaat tcccactttt tttatcaata agtatctcaa ttatactact
      181 cggacaaaat gttgtagaaa taagtgatac taatgcgacg caggaatata aaaaagacaa
      241 tctaatctca gaaatttcta ataaagataa tactcaaatt acaaaaagaa gtgataaaat
      301 taaaagaaca ttttttatta gcttattatt aatatttgct tcttttggct cattaattgt
      361 tattgataca cttttccctg gtctgatact agatatatta ggatatggac aatactattc
      421 aagatatctt tcattgataa tgggtttcgc ttggatggcc aatacaacac tatatttcat
      481 tttcgtttat tttctcaaag catataaaga tagctatatt ttcaaaatgc taataatatt
      541 ctatgccgtt tctctaatag cttcgattat tcttacagga aatgacttaa ataaaattat
      601 tttctatgtt ttgataatac caattattta tattattatt acatcgataa caggttattt
      661 tgttaagaag aaacgtaacc aataaaatgg tataatgatt aattaataac ataaggaaca
      721 ttttatgaga aattattttg aattgcttga ccagtttata tcgacagatt tcaagctaag
      781 atataaaaat tcatacttag gtattttatg gataattttg aaacctcttg cttcttttgc
      841 tgttatttac attatctgga caaatttatt tccacaatca ccagatttta aaatgagctt
      901 attgcttgga ataatgatta tgaatttgtt tagtgaatcg attatgatgg ggatgacttc
      961 gctttcaaca aaagctggaa ttattctcaa agtacgattt ccaagagagg tttcagtttt
     1021 cagcgcagta actatttctc tattcgactt tgcaattaac atgataatat ttactgcttt
     1081 tgcaatttgg aacccaattg atatttcttt tattggaatc atacttttca ttttaagtat
     1141 tttcagcata tattttatta ctaccggtat aagtcttttt ttaagtatta tgtttattag
     1201 gcttcgagat ttgaataatc tcgtttctgt agtactacag ctattttttt ggatgacacc
     1261 agtatattat aaaattgaaa tgctaccaga aaatttacag aaaattattt ctttaaatcc
     1321 aatgacacat attgtcacac tagctcgcag aggacttcta ggagcaagta atattgcaac
     1381 tgatgacttt tggcaaattg gaattgtatt cgtattatca cttattgttt ttggaatcgg
     1441 tgtccttttt ttcaagaaaa gagtatataa aattgctgaa tatttttaat ggaacaaaaa
     1501 ccagtaatag aagtaaagga cgtaagtaaa tcattttatc ttcctcatga aagacatgat
     1561 actcttgttg aataccttac taatccaatt agattatttc gccgaaatgg tgaaatgtat
     1621 caggtattga aagatattga tctcaaagta tacccaggcg agttcttagg gattatgggc
     1681 agaaatggtt gtgggaaatc aacattactt aaaataatag ccggaattta cgcgccaact
     1741 actggtaaaa ttatcattaa tggttcagta gctccatttc ttgaattagg tgtcggattt
     1801 aaccaagaac tatctggtag agaaaatgtg tttttaaacg ggataatact tggaatgaac
     1861 aagaaaaaga tacgacaaaa atatgatgaa attgttcaat ttgcggaatt agaaaaattc
     1921 atggagatgc ctttaaaaaa ctattcatct ggtatgaaag tcaggttagc attctcaatt
     1981 gcaataatga cggatgcaga tatttacatt cttgatgagg tgttatctgt aggagatatt
     2041 gcatttcaaa agaaatgtga tggtgtgttt gcaaaattta agaaagagaa aaaaacagta
     2101 attcttgtaa cacatagtgt caatgcagtt aaagaaatgt gtactagagc agtttttcta
     2161 aaagatggta tcatgcatga ttatcacgat atagataaag ttgttgaagc atatattaat
     2221 ggctagcaag ttgaccctaa aagtaaaaaa atttaaggag ataatacgat ttgagtctat
     2281 tgaattttta aaaattctag gaatcaagaa aatcctttca agcaaggtcg aagcagatag
     2341 aagtgcagtt tctgaaataa atgctgaatc gattgatttt agcagaagag caattattgt
     2401 cctgccaaat attacttggg gatatagaac acaaaggcca caacatattt tctcaaggtt
     2461 agcaaagaag ggctttaata tattttgggt ttcacaattc acttcagata aagaaacagt
     2521 cggaaaaatc gatgagaaca tttatcagtt aaatcttaaa acactcaata agggtaaatt
     2581 gttaagagac tttcacatag atgatgtaaa tgcacaggaa gcttatcaat cattaaaaag
     2641 attaatcggt gggaaattaa agaatgacag tattttgttt acccttcacc ccgcctggta
     2701 ctatctaaca cagaaattta agcaagtcat caagatttat gatctaatgg atctttatga
     2761 aggatttact gattcaaaaa cagatctagt agaaaaagaa aaggaattga tcagccaagc
     2821 tgatttagta tttgcaactg cagacaattt actcaagaat gctaaagatt taaatcctaa
     2881 ttcatttcta attagaaatg gagcagacta tgataatttc aaggaaattt ccccgagtgg
     2941 agttctcgat tgtatttcaa agaagaagat tattggatat tttggatcga tgtcagattg
     3001 gttagatgta aagagtatcg aataccttgc taaaaataat cctgatttaa atttagtttt
     3061 tattggcaaa atcaa
//
LOCUS       DAOSGS010000107         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_30999_length_3004_cov_4.949135, whole genome shotgun sequence.
ACCESSION   DAOSGS010000107 DAOSGS010000000
VERSION     DAOSGS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30999_length_3004_cov_4.949135"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLQ22_04220"
     CDS             <1..585
                     /locus_tag="PLQ22_04220"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOH59199.1"
                     /translation="RFQDETPYMQSVFQFSLHTELSKDALKDINENHYSFLVPDFGDH
                     REECVKALIKEIDLKNGGTVLVYNDTFEKSRIKEFAEIFPQYEKELLKIHDNIVDLMD
                     AFKRKINNKTQVFFYDNKLQGSYSIKKVLPVFAPELSYKDLEIQEGMMAQEAYYSFPK
                     LDEEDLEEVRKNLEEYCKLDTWSMVLIVHALSKL"
     gene            679..1365
                     /locus_tag="PLQ22_04225"
     CDS             679..1365
                     /locus_tag="PLQ22_04225"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH59200.1"
                     /translation="MKPEQEVVTIDEEKAKKEKVKAVFGWIGYVASLVVLAYLIFYFV
                     FNLVSPNLTARYMGFKGYVILTESMAPELVPNDFVFITAYDFDDLKEGDIVAFVDPNN
                     NVVVHYFAEWTVNERGDACFRTRPADPEKPMDYWRITKDKFVGIYSGRIPKVGGFLRV
                     VGSPIGLIAIVVIGVSIYLIVHIFKKPTKEKQEEIAQAGAPEENIIEAEAEVVKEEPK
                     LEEEKKESKE"
     gene            1458..2417
                     /gene="guaA"
                     /locus_tag="PLQ22_04230"
     CDS             1458..2417
                     /gene="guaA"
                     /locus_tag="PLQ22_04230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOH59201.1"
                     /translation="MKSNPNLSLHQYLEKQIKEIRAEVGTKKVLLGISGGVDSTVVAK
                     ILHLAIKDQLTCLFINNGLLRLNEEKQVLERFEKYGIEVEYLDASKIFLDNLQGVKDP
                     EEKRKIIGKIFIDCFKEKAKSLGKEYEYLAQGTIYTDVIESKKGEQLVKSHHNVGGLP
                     SELGFKLVEPIKELYKDEVRALGKIMGLDDELLLRQPFPGPGLGVRVMGEITNEKLQM
                     AKISDAIFREELKKAKLDQIIWQYFTVVLDTKSTGIFDGKRNYNYVIALRAVISKDAQ
                     TAQAYPIPYEILSKIGERIVLETKCSRVIYDISSKPPGTIEWE"
     gene            2420..>3004
                     /locus_tag="PLQ22_04235"
     CDS             2420..>3004
                     /locus_tag="PLQ22_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59202.1"
                     /translation="MKRKVLFVFLLAVFGVLFFNYKVEAKSFEEFYNDYASMNFSQKV
                     NALHNSQEIGNKFYYNTLDEYEKEIYDAFLSAIRVGYPFEVVIEQQLGDTLQDLYNAA
                     FEAWKAFNLDHPEYFWFNNNIEPDTSRLGFLIVKPRIEVGYLNDGEYNQSLVDEDLIH
                     ILARRAYLKQEVDLLQNDYQKLRYLYTWLIQNNEY"
BASE COUNT         1133 a          313 c          558 g         1000 t
ORIGIN      
        1 cgttttcaag atgaaacgcc ttatatgcaa tctgtgtttc agttttcttt acatacagag
       61 ctttctaaag atgctttaaa agatattaat gaaaatcatt attcgttttt agtccctgat
      121 tttggagatc atagggaaga atgtgtgaaa gcattaatta aagaaatcga tttaaaaaat
      181 ggtggtactg tgcttgtata taatgacact tttgaaaaat caagaataaa agaatttgcg
      241 gagatttttc ctcaatatga aaaagagtta ctaaaaatac acgataatat agtagattta
      301 atggacgctt ttaaaaggaa aataaataac aaaacacaag tatttttcta tgataataag
      361 ttgcaaggaa gttatagtat taaaaaggta ttaccagttt ttgcgcccga attatcttac
      421 aaagatttag aaattcaaga agggatgatg gcccaagaag cttattattc ttttccaaaa
      481 ctagacgaag aagatttgga agaagtaaga aagaatttag aagagtattg taaattggat
      541 acttggagca tggttttaat agttcatgcg ctatcaaaac tataatgtaa gcattttact
      601 tatatgtaaa gaataatttt tttgttgttg acaaaactaa tttattatgt aataatatat
      661 gcgtgaggga ggttcgttat gaaaccagaa caagaagtag taacaatcga tgaagaaaaa
      721 gccaaaaaag aaaaagttaa agctgttttt ggttggattg gttatgttgc ttcactagta
      781 gtattagctt atttaatttt ttattttgta tttaacttag tgtcacctaa tttaactgca
      841 agatatatgg gtttcaaagg ctatgtaatt ttgacagaaa gtatggcgcc agaattagtt
      901 cctaatgatt ttgtattcat tactgcctat gattttgatg atttaaaaga aggcgatatt
      961 gttgcttttg tagatcctaa taataatgtg gtagttcact attttgctga atggactgta
     1021 aacgaaagag gagatgcatg tttccgtaca cgtccagctg atcctgaaaa accaatggat
     1081 tattggcgta ttacaaaaga taaatttgta ggaatttatt caggaagaat tccaaaagtt
     1141 ggtgggttct taagagtagt aggatctcct ataggtttaa ttgcaattgt agttattgga
     1201 gtatcaattt atttaattgt ccatattttt aaaaaaccaa ctaaagaaaa acaagaggaa
     1261 atagctcaag ctggggctcc agaagaaaat ataattgaag cagaagcaga agttgtaaaa
     1321 gaggaaccta agcttgaaga ggagaaaaaa gagtctaaag aataaagatt taaagagtta
     1381 acattttcaa atttatgtta actttttttt atttatttct atagttaatt gtgctataat
     1441 aaatgatgag gtgttttatg aaatcaaatc caaatcttag tttacatcaa tatttagaaa
     1501 aacaaattaa agaaataagg gcagaagttg gaactaaaaa agtactttta ggaatttctg
     1561 gtggtgttga ttcaaccgtt gtggcaaaga ttttacacct tgcaattaaa gaccaactaa
     1621 cttgtttgtt tatcaataac ggattacttc gtttaaatga agaaaaacaa gttttagaga
     1681 gatttgaaaa atatggaatt gaagtagaat atttagatgc atctaaaata tttttagata
     1741 atttacaagg agttaaagat ccagaagaaa aacgaaaaat tattgggaaa atatttattg
     1801 attgttttaa ggaaaaagca aagagtttgg gtaaagaata tgaatattta gctcaaggaa
     1861 cgatttatac tgatgttatt gaatctaaaa aaggagaaca attagttaaa tcgcaccaca
     1921 atgtaggggg attacctagc gaattagggt ttaagttagt agagccgatt aaagaattat
     1981 ataaagatga agttagagca ttaggtaaaa taatgggttt agatgatgaa ctattattaa
     2041 gacaaccttt tcccggccca ggtttaggag taagagttat gggtgaaatt actaatgaaa
     2101 aacttcaaat ggccaaaata agtgatgcta tttttagaga agaattaaaa aaagcaaaat
     2161 tagaccaaat tatttggcaa tattttactg ttgttttaga tactaaatca acggggattt
     2221 ttgatggtaa gaggaactat aattatgtta ttgctttaag agcagtaata agtaaagatg
     2281 ctcaaacagc tcaagcttat cctatacctt atgaaatatt atctaaaatt ggcgagagaa
     2341 ttgttttgga aacaaagtgt agtagagtaa tctatgatat ttcttcaaaa cctccaggaa
     2401 caatagaatg ggagtaaaaa tgaaaagaaa agtattattt gtatttttgc ttgctgtttt
     2461 tggtgtatta ttttttaatt acaaagtaga agcgaaatca tttgaagagt tttataatga
     2521 ttatgcaagt atgaatttta gtcaaaaagt taatgcttta cataatagtc aagaaattgg
     2581 gaataaattt tattacaata ctttagatga atacgaaaaa gaaatttatg atgctttctt
     2641 atctgcaatt agagtaggat atccttttga agtcgttata gaacaacaac ttggtgatac
     2701 gttgcaagat ttatataatg ctgcttttga agcttggaag gcctttaatt tagatcatcc
     2761 agaatatttt tggtttaaca ataatattga gccagatact tcaagattgg ggtttttgat
     2821 tgttaagcca agaattgaag ttggctattt aaatgatgga gaatataatc aatcattagt
     2881 tgatgaagat ttaattcata ttttagctcg tagagcgtat ttaaaacaag aagttgattt
     2941 acttcaaaat gattaccaga aattaagata tttatatact tggctaatac aaaacaatga
     3001 atat
//
LOCUS       DAOSGS010000108         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_31381_length_2979_cov_3.773256, whole genome shotgun sequence.
ACCESSION   DAOSGS010000108 DAOSGS010000000
VERSION     DAOSGS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31381_length_2979_cov_3.773256"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PLQ22_04240"
     CDS             complement(<1..1242)
                     /locus_tag="PLQ22_04240"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59203.1"
                     /translation="MMFAEVVVDVKNSAVDMLFTYEIPEHFKSYVDIGSRVLVEFGTR
                     QVIGYVIRISDETSYAGEIKEILDVYDFDKQLTEEQIELAKKIAFETKSLFISALELM
                     YPAFLKSKYRKYLKVVNYEQLDSNIALLFRGKSKLEYNRTIVEYSNQIAKELNKGNLA
                     IEYDQHTYGKNKYQNIYFVSKDKDAKNLTVKRKRIMEYVLNNPGQTIEEVSLNTSSSH
                     VLINDLRKEGFLEVREVIKEEDNLKKFQASKVTYNPLEQDIKAKYDSNKNKPYLLITD
                     DFEFRLKFLEDVISKTIMSNRQVLILAKSIVQSFEIYNFLEKELINSRTYCFNSSLSN
                     NEYFDNYVQVKKGNVDVVVGTISSSFLPLENLGLIIIFDSNNSEYINDYSPKFSTLNV
                     LKWRMEYHKAELILVNETLSVE"
     gene            complement(1239..2105)
                     /locus_tag="PLQ22_04245"
     CDS             complement(1239..2105)
                     /locus_tag="PLQ22_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH59204.1"
                     /translation="MLIGAIEAGGTKFKCAVATSSGVILQDTTIKTTTVEETMEKVIK
                     FFLAFPIKTMGVGCFGPICLDPSDERYGMILDTPKKIWANYNILKALKDALKCNVKIT
                     TDVNASAFGEYYYGYKKKFQNLVYVTIGTGIGGGIVYNGSLVQGHHHPEIGHMTLKRD
                     SNDNYPSTCPFHSDCFEGLASGISLDKRYNLSYDELAKRDDLLRREANYIAQGIYNLY
                     LTFAPDKIIIGGGVSHQEKLLPLVREEFEKLNNNYYTYKAVKSVDELIVSPTLKDNSA
                     LYGAIALGVIEK"
     gene            complement(2219..2470)
                     /gene="rpoZ"
                     /locus_tag="PLQ22_04250"
     CDS             complement(2219..2470)
                     /gene="rpoZ"
                     /locus_tag="PLQ22_04250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOH59205.1"
                     /translation="MKKEKNIGEGLRYPPIDALLDKSKSKYVLVLGAAKRAKDLEHGD
                     AEELLEDPVNKKSLGLALEEIYEDQITIYDSTNEETLGK"
     gene            complement(2470..>2979)
                     /gene="gmk"
                     /locus_tag="PLQ22_04255"
     CDS             complement(2470..>2979)
                     /gene="gmk"
                     /locus_tag="PLQ22_04255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH59206.1"
                     /translation="SISMTTRKQRAGEVDGKDYFFVSKEEFEEKIKNGEMLEYAEFVG
                     NYYGTPLNYVKEQLNEGREVVLEIEIQGASQVKCKMPHAVFIFIAPPSLKALHDRLTS
                     RGTESEELIQKRFDKALSEMHMAYMYDYIVVNDDIHNAAEKIMTIIRAEHARTERTIH
                     GYKKLLEEE"
BASE COUNT          988 a          552 c          306 g         1133 t
ORIGIN      
        1 ttcaacactt aaagtttcat ttactaaaat taattctgct ttatgatatt ccattctcca
       61 ttttaaaaca ttaagcgtag aaaatttggg actataatca ttaatatact cagaattgtt
      121 tgaatcgaaa attataatta aacctaaatt ttctaaaggt aaaaaactgc tagaaattgt
      181 ccctactact acatctacat tacctttttt aacttgcaca taattatcaa aatattcatt
      241 attgcttaac gatgaattaa aacaatacgt tcttgaatta attagttcct tttctaaaaa
      301 attatatatt tcaaaagatt gaacaataga tttcgccaat attaacactt gtctattgga
      361 cataattgtt tttgaaataa catcttctaa aaattttaat ctaaactcaa aatcatcagt
      421 tattaaaagg taaggtttat tcttattaga atcatattta gctttaatat cttgttctaa
      481 aggattataa gtaactttag atgcttgaaa tttctttaaa ttatcttctt ctttaataac
      541 ttcccttact tctagaaaac cttcttttct taaatcgtta attaaaacat gactagagct
      601 agtatttaaa ctaacttctt caatagtttg acctggatta tttaaaacat attccataat
      661 cctttttctt tttacagtta gatttttagc atctttgtcc ttacttacaa aataaatatt
      721 ttgatatttg ttttttccat aagtatgttg atcatattct attgctaaat ttcctttatt
      781 taattcctta gctatctgat tactatattc tactatagta cgattatatt cgagcttgga
      841 tttcccacga aaaagcaaag caatattact atctaattgt tcataattaa caacttttaa
      901 gtatttacga tatttacttt taagaaatgc aggatacatt aattctaatg cagaaataaa
      961 taaacttttt gtttcgaaag ctattttttt ggcaagttca atttgttctt cagtaagttg
     1021 tttatcaaaa tcataaacat ctaatatttc tttaatttca ccagcatatg aagtttcatc
     1081 actaatccta attacataac cgataacttg acgagtacca aattcaacta ataccctact
     1141 tcctatatct acataagatt taaaatgttc aggaatttca taagtaaata acatatcaac
     1201 tgcagaattc ttaacatcaa ccacaacttc tgcgaacatc atttctcaat cacccctaaa
     1261 gcaattgccc catataaagc tgagttatct tttaaagtag gacttacaat taactcatca
     1321 acacttttaa ctgctttata tgtatagtaa ttgttattta atttttcaaa ttcttctctt
     1381 actaaaggaa gtagcttttc ttgatgacta actccaccac cgataattat tttatcaggt
     1441 gcaaatgtta gatataaatt atatattcct tgagcgatat aattagcttc tcttcttaat
     1501 aaatcatctc ttttagctag ttcatcatag cttaaattgt atcttttatc taaactaata
     1561 ccacttgcta gtccttcaaa acaatcacta tgaaaaggac atgtactagg atagttatca
     1621 ttagaatctc tttttaaagt catatggcct atttccggat gatgatgacc ttgaactaga
     1681 cttccattat aaacaattcc tccaccaatt cccgtaccta tcgttacata aactaagttt
     1741 tgaaacttct ttttatagcc ataatagtac tcaccaaaag ctgaggcatt gacatcagta
     1801 gttatcttga cattacattt aagagcatct tttaatgcct ttagaatatt ataattagcc
     1861 caaatcttct ttggtgtatc taaaatcatc ccatatcttt catcactagg atctaaacaa
     1921 atcgggccaa aacaaccaac acccattgtc ttaattggaa aagcaagaaa aaacttgatt
     1981 actttttcca tcgtctcttc tactgtagtt gttttgatag tagtatcttg taagataaca
     2041 ccacttgatg tagctacagc acatttgaat ttagtaccac cagcttcaat tgctccaatc
     2101 aacatacgaa tagccctcct tttacaatat tttaccatat tttatcaaaa aaataaacta
     2161 ctactaatat aataactact tgccttattt tttactttta tattttaaaa agaaacactt
     2221 atttcccaag tgtttcttca ttagtactat cataaattgt aatttgatct tcataaattt
     2281 cttctaaagc caaacccaaa gattttttat ttactggatc ttctaataat tcttcagcat
     2341 ctccatgttc taagtcttta gctcttttag ctgcacctaa aactaaaaca tacttactct
     2401 tacttttatc taataaagca tcaattggtg gatatcttag tccttcacca atattctttt
     2461 cttttttcat tattcctcct ccaaaagttt tttataacca tgtattgttc tttctgtcct
     2521 agcatgttca gctctaatga ttgtcattat tttttcagct gcattatgaa tatcatcatt
     2581 aactacgata taatcataca tatatgccat atgcatctca ctcaaagctt tatcaaaacg
     2641 tttttgaatt agctcttctg attcagttcc ccgactagtc aagcgatcat gtaaagcttt
     2701 taggctaggt ggagcaataa aaataaatac agcatgaggc attttacatt taacttgtga
     2761 tgcaccttga atctcaattt ctaaaacaac ttcacgacct tcattaagtt gttccttaac
     2821 atagttaagc ggtgttccgt aatagttccc aacaaactcc gcatattcaa gcatctcacc
     2881 atttttgatt ttctcttcaa attcttcttt tgacacaaaa aagtaatcct tgccatcaac
     2941 ttcgccagct ctttgttttc tagtagtcat cgaaataga
//
LOCUS       DAOSGS010000109         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_32230_length_2926_cov_8.002090, whole genome shotgun sequence.
ACCESSION   DAOSGS010000109 DAOSGS010000000
VERSION     DAOSGS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32230_length_2926_cov_8.002090"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1605)
                     /locus_tag="PLQ22_04260"
     CDS             complement(157..1605)
                     /locus_tag="PLQ22_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59207.1"
                     /translation="MADTKSTFYNLLLTRTIPDENLAEITDFAYLIFIRQPGTKRATY
                     AIAQANIPTATLVVIEAEISAGSFPDYQLQIRHVKDNNENLIYSSYVKFILPKNYNQN
                     KAGTTVKGTSPTVPVQFILVNPVIWEMSTNNSFNKTFNDKRYTALEILELYTKEIIKK
                     YSSSSQRLTWDKICDLTTKNKTLYDQVIIPSTITELQVPEYIIGAYKIFSTPSLWLFD
                     NFDFSIKEIENSKATGKIPIIGLLLNFYNALECFEKYDISKDADIKQFTTLLKIIPYN
                     DTLSVLSKPNAVVNFKNTTNMTQVTRLFGNLPKVTLSDNTKETQSQRVASLEIEYTDN
                     LEEAETRIVDCVDLMQKQINQIEIYETINTSPEWLQFGYLYNLERDKKTGDNLNRYIH
                     TPLSIFNIFLRQEQTIGKMEHIIKYAMLRLNDKNVAGSGEWVCDKSEQANGSCNKTDC
                     PHYKNHEKLPQQCPNDKDISCPFNKFAHAKCK"
     gene            complement(1671..1865)
                     /locus_tag="PLQ22_04265"
     CDS             complement(1671..1865)
                     /locus_tag="PLQ22_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59208.1"
                     /translation="MKNEDIKIIKDCLLKYGDDFWKDEDNEIELNDGVITSNVELIYD
                     VIEAINKAGYDLKLIKLKRK"
     gene            complement(1855..>2926)
                     /locus_tag="PLQ22_04270"
     CDS             complement(1855..>2926)
                     /locus_tag="PLQ22_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59209.1"
                     /translation="LPEKKIAFKFNKMSAEAEVETVEWETSRTGRIIPVVKFKPIELG
                     GATIQRATGFHAKFIYDNQIGPGAIVKIVRSGDVIPYIDDVIKKSDVVGELIICPSCG
                     YHPLSTDDTQTHIYCTNPNCPTQVQKKIAYFFEKLGLENFSEKMISTLGCINVMDIYN
                     LKKEDIIKIEGWAETSADDFIKRIEQTKKAKPERILAALGVDNLGITTAKLVLENFSI
                     SDILNTLDNPDALRQMVFKLIQVKGLGSKKVASIVKGLKENKTLLENFIALAGEVTEN
                     IGPLSGKSFCITGSLSKPRKAYEQVIEKFGGSNTSISSCDYLICNTPSDSNKFKKAIE
                     NGVKIINEQELIELIKGSKNEK"
BASE COUNT          898 a          475 c          384 g         1169 t
ORIGIN      
        1 ttttcttgag tttttggagc atctcgtgaa ttaaagaata tttaagctga tatttgatat
       61 aataattcat atcattattg atctcctaaa aacaaaaaga ttttaaattc atagatatta
      121 tttctatata aaaattgaaa aaaataaaaa tgtcaattac ttgcatttgg catgcgcaaa
      181 tttattaaat gggcatgaaa tatctttatc atttggacat tgttgtggta atttttcatg
      241 atttttataa tgagggcaat ctgttttatt gcaactacca tttgcctgct cagatttgtc
      301 gcaaacccat tctccactac cagcaacatt tttatcatta agtctcaaca ttgcatattt
      361 tataatatgt tccattttac caattgtctg ctcttgccgg agaaaaatat taaaaataga
      421 taacggagtg tgaatatatc tatttaaatt gtcaccagtc tttttgtctc tttcaagatt
      481 atacagataa ccaaattgta accactctgg agaagtgtta atagtttcat atatttcgat
      541 ttgatttatt tgtttttgca ttaaatctac acaatcaaca attctcgttt ctgcttcttc
      601 tagattatct gtatattcaa tttctaatga tgctactctt tgagattgag tttctttagt
      661 attatctgat aaagttactt ttggcaaatt tccaaataaa cgagttacct gagtcatgtt
      721 tgtagtgttt ttaaaattca caactgcatt tggtttgctt aaaacagaca gagtatcatt
      781 atatgggatt atttttaaaa gagttgtaaa ttgtttaatg tctgcatctt ttgaaatatc
      841 atatttttcg aaacattcta aagcgttata aaagtttaat aataatccta ttattggtat
      901 tttgccagtt gctttagaat tttctatttc ttttatagaa aaatcaaaat tatcaaataa
      961 ccataggctg ggtgtactaa aaattttata tgctcctatt atatattctg gaacttgcaa
     1021 ttctgtaatt gttgatggaa ttatcacttg atcatataag gttttatttt ttgttgttaa
     1081 atcacatatt ttatcccatg ttaatctttg tgaagaactt gagtatttct taataatctc
     1141 cttcgtgtat aattctaaaa tttctagtgc agtatatctt ttatcattaa atgttttatt
     1201 aaatgaattg tttgtactca tttcccagat cactggattg accaaaataa attgtactgg
     1261 gacagttgga gaagttcctt ttacggttgt tcctgcttta ttttgattat aattttttgg
     1321 taatataaat ttgacatatg atgaataaat tagattttca ttattgtctt ttacgtgtcg
     1381 aatctgtaat tggtagtctg gaaatgatcc cgcactaatt tcagcttcga ttacaaccaa
     1441 tgttgctgtt ggaatattag cttgcgcaat agcatacgtt gctcgttttg ttcctggttg
     1501 tcgaataaat attaaatatg caaaatccgt tatctctgca agattttcat ctggtatagt
     1561 tctcgttaat aaaagattat aaaaagttga tttagtgtct gccatgattt gctcctaaaa
     1621 aaattgtatc ggcaagatct tatttagatc ttgccgataa taaattatat ttactttctt
     1681 ttcagtttaa ttaattttaa atcataaccc gctttattga tagcttctat aacatcataa
     1741 attaactcta catttgatgt tataactcca tcatttaatt caatttcatt atcttcatct
     1801 ttccaaaaat catctccata ctttaaaaga caatctttga tgattttgat atcttcattt
     1861 ttcattttta cttcctttta tcagctcaat taattcttgc tcatttatta ttttaacacc
     1921 attttcaatc gcctttttaa atttgtttga atcacttggt gtattacaaa ttaaatagtc
     1981 acaagaactg atacttgtat ttgatccacc aaatttttca attacttgct cataagcctt
     2041 tctcggctta cttaaactac cggttataca gaaactttta ccagacaatg gtccgatatt
     2101 ttctgttact tccccggcaa gagctataaa gttttctaaa agagttttat tttcttttag
     2161 tcctttaacg atagacgcta ccttctttga tccaagtcct ttaacctgaa tcaatttaaa
     2221 taccatttgt cttaatgcat ctggattatc caatgtattt agaatatcag atattgaaaa
     2281 attctccaaa actaatttag cagtggttat tccaagattg tcaacgccaa gtgctgctaa
     2341 aattctttct ggtttagctt ttttagtttg ttcaattcgc tttataaaat cgtctgcaga
     2401 tgtttccgcc catccttcaa ttttaataat atcctctttc ttaagattat aaatgtccat
     2461 aacatttata caccctaaag tagatatcat tttttcacta aagttctcaa gtcctaattt
     2521 ctcaaagaaa tacgcaatct ttttctgaac ttgagttgga caatttggat ttgtgcaata
     2581 tatatgtgtc tgagtatcat ctgttgataa tggatgataa ccacatgatg gacatatgat
     2641 taattccccc acaacatcag attttttaat tacatcatca atatatggaa tcacatctcc
     2701 agatcgaaca atctttacaa ttgcgcctgg accaatctga ttatcataaa tgaattttgc
     2761 atgaaatcct gtagctcgtt gaatggttgc tccaccaagt tcaattggtt taaattttac
     2821 aacaggaata attcttccag ttctggaagt ttcccattca actgtttcaa cttcagcttc
     2881 tgccgacatc ttattaaact taaaagcaat cttcttctca ggaagt
//
LOCUS       DAOSGS010000110         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_32663_length_2899_cov_4.736990, whole genome shotgun sequence.
ACCESSION   DAOSGS010000110 DAOSGS010000000
VERSION     DAOSGS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32663_length_2899_cov_4.736990"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLQ22_04275"
     CDS             <1..1396
                     /locus_tag="PLQ22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOH59210.1"
                     /translation="KNLSLEIDITNDGTILVTEDWDIEFLKETNEFYRNIYYHKLEED
                     NPFVANHEAIGNIATFSTIPNTYNQRLLKDNEELEIEKGYSFNHDLDKNGEVIGGINR
                     INLEVFYFQTNDVLMGDYRIQYQYKLEYFITNYLDCNELSLNLIQDPILINQINFKIN
                     FPKSISKPFTYSNHQLSSSFLDNSLNIEVSDFYYFDNDSFRIRVLFDQDSLAIANPNQ
                     IMNFQAFTDLNNYEALKQETNQTKIAVYISNIVLVLVTIVIMVLVYLYFENIIFKAKV
                     SSKVMLIDDGLLESSLILDGFSRKRHFLGLIKKMELNGNITRENNVLTYKHRNGLSKQ
                     ETKILNTIFEKQKTIALEKINYSKLKKLATSGSEYEKKPQLILLNFLFIFIFYYIIIY
                     KMEDKLDLDFIWTKILIALTFLVSLVFSLLIRLKKRKKYKEVKAFKNYLEEAKLKDDI
                     YLPYYISYGIIDFK"
     gene            1414..1953
                     /locus_tag="PLQ22_04280"
     CDS             1414..1953
                     /locus_tag="PLQ22_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH59211.1"
                     /translation="MSAGWIITFVVIGLIIIGLVVLIILIYDSLVKARKRVNNGWNQI
                     KLYVERRFDLVPNLMETLKDVISEDEGILSEFARVRGIYANSNTPMEIAKADFLLTNV
                     LDRLVIVQEGYPSIKKNGSFQRLLFDLEDDKVKIVYSQSYFNDAVDKYNLKLVKFPSK
                     IVAGWMKLEPQEKYEYDYK"
     gene            complement(2021..2236)
                     /gene="rpmE"
                     /locus_tag="PLQ22_04285"
     CDS             complement(2021..2236)
                     /gene="rpmE"
                     /locus_tag="PLQ22_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOH59212.1"
                     /translation="MKKGIHPKYHKATVTCTACGATFETGSTVEDIKVDTCSKCHPFY
                     TGQQKFVQAAGRVEKFQKRLAKKEEDK"
     gene            2465..>2899
                     /locus_tag="PLQ22_04290"
     CDS             2465..>2899
                     /locus_tag="PLQ22_04290"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH59213.1"
                     /translation="MEVQRRDGWIEVICGSMFAGKTEELLRRLKRLEYAKKSYMVFKP
                     AIDNRYSETEVVSHNNKRVAGYNISDETQILELVAKGNPDVVAIDEAQFFTDKIVEVC
                     ETLADQGKRVIVAGLDTNFRGEPFGPMPYLLARAEYVTKLQAI"
BASE COUNT         1072 a          307 c          475 g         1045 t
ORIGIN      
        1 aaaaaattta tccttagaaa tagatataac aaacgatgga acaattttag ttactgaaga
       61 ctgggacata gagtttttaa aagaaaccaa tgagttttat cgtaatattt attatcataa
      121 actagaagaa gataatcctt ttgttgctaa tcatgaggca attggtaata ttgctacttt
      181 ctctacaatc cctaatactt ataatcaaag attattaaaa gataatgaag aactagagat
      241 tgaaaaaggt tattctttta atcatgattt agataaaaat ggtgaagtta ttggtggtat
      301 taaccgaatc aatttagaag tcttttattt tcaaacaaat gatgttttaa tgggtgatta
      361 tcgcatccaa tatcaatata aactagaata ttttattact aattatttag attgtaatga
      421 actgagttta aatttaattc aagatccaat tttgattaat caaatcaact ttaaaattaa
      481 ttttcctaaa tctatttcta aaccatttac ttatagcaat catcaattaa gcagcagttt
      541 tttagataat agtttaaata ttgaagtaag tgatttttat tattttgata atgacagttt
      601 tagaattaga gttttgtttg atcaagattc gctagcaata gctaatccta accaaattat
      661 gaattttcaa gcctttacgg acttaaataa ttatgaagct cttaaacaag aaacaaacca
      721 aacaaaaatt gctgtctata ttagtaatat agttttagtt ttagttacaa ttgttattat
      781 ggttttagtt tatttgtatt ttgaaaatat tatttttaaa gcaaaagtta gttctaaggt
      841 aatgctaatt gatgatgggt tacttgaatc tagtttaatt ttagatggtt tttctagaaa
      901 aagacatttt ttaggtttaa ttaaaaaaat ggaattaaat gggaatatta ctagggaaaa
      961 taatgttcta acatataaac atagaaatgg tctttcaaaa caagaaacaa agattttaaa
     1021 taccatcttt gaaaaacaaa agacgattgc tttagaaaaa attaattatt cgaaattgaa
     1081 aaagttagcg actagcggta gtgaatatga aaagaagcca caattaattt tattgaattt
     1141 cttgtttatt tttatctttt attatataat aatttataag atggaagaca aattggatct
     1201 tgattttatt tggactaaga ttttaattgc tcttacattt cttgtttcac ttgtattttc
     1261 tcttttaatt cgtttgaaaa agagaaagaa atataaagaa gtaaaagctt ttaaaaatta
     1321 tttagaagaa gctaaattaa aagatgatat atatttacca tattatatta gttatggtat
     1381 tattgatttt aaatagaaaa ggaggtgttt ctaatgagtg ctggttggat tattacattt
     1441 gtagttatag gacttataat tataggacta gttgtcttaa ttattttaat ttatgatagt
     1501 ttagtaaaag cacgtaaaag agtaaacaat ggctggaatc aaattaaact atatgtagaa
     1561 cgaagatttg atttagttcc taatcttatg gaaacactga aagatgtaat ttctgaagac
     1621 gaaggtatct taagtgagtt tgcaagggta agaggaattt atgcaaattc taatacacct
     1681 atggagattg ctaaggcaga cttcttatta actaatgttt tagatagatt agttattgtt
     1741 caggaaggtt atccttcaat taaaaagaat ggtagttttc aaagattatt gtttgattta
     1801 gaagatgata aagttaagat tgtatatagt caatcatatt ttaacgatgc agttgataaa
     1861 tacaatttga aactggtaaa gtttccaagt aaaattgtag ctggttggat gaaactggag
     1921 ccacaagaaa aatacgaata tgactataaa taaaaacact ggaattaacc agtgtttttt
     1981 aatagatata aaaaaagcaa catgattgtt gcaattttta ttacttatcc tcttcttttt
     2041 tagccaaacg tttttggaat ttttcaactc ttcctgctgc ttgtacgaat ttttgttggc
     2101 cagtgtaaaa cggatgacat ttagaacaag tatcaacttt gatatcttct actgtagatc
     2161 cagtttcaaa agtagcacca caagctgtac aagtaaccgt tgctttgtga tatttaggat
     2221 gaataccttt tttcattttt ctctccttcc gccatggttt ttatttgcaa accatagtaa
     2281 gttttatact atgttattat accttaaagg tctgtttttg tcaacaagaa taaacaaatt
     2341 tgaaaataaa aagctgggat aattagtttt ttaagattta ttattccttc tatttcacat
     2401 ctaaaatatt tgaaaaagag aataaatatg gtataatatt atagaaagga ttagaagagt
     2461 aaagatggaa gtacaaagaa gagatggttg gatagaagtt atctgtggaa gcatgtttgc
     2521 gggaaagact gaggaattat taagaagatt gaaacgatta gaatatgcta agaaatcata
     2581 tatggttttt aaaccagcaa ttgataatcg ttatagcgaa actgaagtag taagtcataa
     2641 taataaacgt gttgcaggat ataatatttc ggatgaaaca caaattttag aattagttgc
     2701 taagggaaat ccggatgtag ttgctattga tgaggcacaa ttctttactg ataagattgt
     2761 tgaggtttgt gagactttag ctgatcaagg taaaagagta attgtagctg gcttagatac
     2821 aaatttcagg ggtgaacctt ttggaccaat gccttattta cttgcaagag cagaatatgt
     2881 aactaaactt caagcaatt
//
LOCUS       DAOSGS010000111         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_34922_length_2769_cov_2.965365, whole genome shotgun sequence.
ACCESSION   DAOSGS010000111 DAOSGS010000000
VERSION     DAOSGS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34922_length_2769_cov_2.965365"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /gene="phoU"
                     /locus_tag="PLQ22_04295"
     CDS             <1..503
                     /gene="phoU"
                     /locus_tag="PLQ22_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOH59214.1"
                     /translation="QLEKVIQEQCVDLMVSFTPLARDLRVILGILKLITDLERIGDHA
                     EDIAIFSNALNKHEYKQLDQVKEMADISTNMIRLAIDAFVKDDLELAEKVIKQDDKID
                     KLYFTLLKQLMETKNLSNEVIVYHTLILRYLERTADHAVNVAEWVIYIISGYHKSNPD
                     GRFTYV"
     gene            496..1179
                     /locus_tag="PLQ22_04300"
     CDS             496..1179
                     /locus_tag="PLQ22_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH59215.1"
                     /translation="MSRLIYSVEDDQNISFVINKTLTNSGFKVKSFSDGESFFKGLED
                     EIPDLALVDIMLPNGMSGLDIIKKMKANSKYDDVVIIILSAKGDEMDKVTGLDLGADD
                     YISKPFSVLELVSRIKANLRKRPKKKDILSLANITLNFLEHTCMKDGKLVDLTLKEFD
                     LLKYFMENPNKALSRDELLNNVWGYDYIVETRTVDMHVKTLREKLGDSKDKALIESIR
                     SIGYKFIVK"
     gene            1176..2468
                     /locus_tag="PLQ22_04305"
     CDS             1176..2468
                     /locus_tag="PLQ22_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOH59216.1"
                     /translation="MKKRLLSRAILLFFLVSVIVFFVTVVIINHNNETAAEREVINYT
                     HILKRQYASGEHDKLITEVKKVDPDIRVTVIDSDGVVLGDSFSDEITENHLDRPEIRN
                     LGKTYIRYSNTYKKTYLYYATLLDDGNYLRIALSFKYYQMNLYLFLTLLALFIASGVI
                     LYFMLDKQIGTTIAPLNEQIEELSSIAGYKGGEFEEDEIIKLTNQVAYIHQILKSNLE
                     AIKTEAEKVNYIVDALPLGVIVLNNKGEIKQINEYGKKLFNYSANITEYLYLSRVEEF
                     NNLIKESLEKKTSMQADCLLDNKIYNVSTKVITNEWLNEGLVVIVSDIDLVKRLEKAK
                     RDFFANASHELKSPLTSIIGLQEMIENNFVSSKEEFEDINKRTISEARRSMKLINDML
                     SLAELESLKAKPMDQEVDLKVLVLDVLKSLDVKIKAKG"
BASE COUNT         1064 a          284 c          510 g          911 t
ORIGIN      
        1 accaattaga aaaagtaatc caagagcaat gtgttgactt aatggtatct tttacgccgc
       61 ttgcaagaga tttgagagtt attttaggta ttctaaagtt gattacagat ttagagagaa
      121 taggcgatca cgcagaagat atcgctattt tctcaaacgc tttaaataaa catgagtata
      181 aacagctaga tcaagttaaa gaaatggctg atatttctac gaatatgatt agactagcta
      241 ttgatgcttt tgtaaaggat gatttagaac ttgcagagaa agttattaaa caagatgata
      301 aaattgataa actttatttt acattactaa aacaattaat ggaaacaaaa aatttaagta
      361 atgaagttat tgtttaccat actttaatct taagatattt agaaagaact gcagatcatg
      421 cagttaatgt agccgaatgg gtaatctata ttattagtgg ttaccataag agtaaccctg
      481 atgggaggtt tacatatgtc tagattaatt tattcagtag aagatgacca aaatattagt
      541 tttgtgatta ataagacact tacaaactca ggttttaagg taaagtcatt ttctgatggg
      601 gagtcatttt tcaaaggatt agaagatgag attcctgatt tagctttagt tgatattatg
      661 cttcctaatg gtatgagtgg tttagatatt atcaagaaga tgaaagcaaa tagtaaatat
      721 gatgatgttg ttatcattat tttgtcagct aaaggtgatg aaatggacaa agtaacaggg
      781 ctagatttag gtgcagatga ttatattagt aaaccattta gtgttttaga gttagttagt
      841 agaatcaaag ctaatttaag aaaaagacca aagaaaaaag atattttaag tttagctaat
      901 attactttaa actttttaga gcacacatgt atgaaagatg gaaaattagt tgatttaact
      961 ttaaaagaat ttgatttatt aaagtacttt atggaaaacc ctaataaagc tttatcaaga
     1021 gatgaacttt taaacaatgt ttggggttat gattatattg tagagacaag aacagttgac
     1081 atgcatgtca agactttaag agagaaatta ggagatagta aggataaagc attaattgaa
     1141 tcaataagat caattggcta taagtttata gtaaaatgaa aaagagatta ttaagtagag
     1201 cgattttact tttctttctc gtttcagtaa tagttttctt tgtaactgta gttatcatta
     1261 atcacaataa tgaaacagca gcagaaagag aagtaattaa ctatactcat attttaaaaa
     1321 gacaatatgc aagtggagaa catgataagt taattactga ggtaaagaaa gtagatccag
     1381 atattagagt tactgttatt gatagtgatg gtgttgtctt gggtgatagt ttttctgatg
     1441 agataacaga aaatcattta gacagaccag aaattaggaa tttagggaaa acatatattc
     1501 gttattctaa tacttacaaa aaaacttatc tttactatgc tacattgtta gatgatggca
     1561 attatttaag gatagcttta agttttaagt attaccaaat gaatttgtat ttgtttttaa
     1621 cgcttttagc actttttatt gcatcaggtg taatacttta ctttatgcta gataaacaaa
     1681 taggaacaac tattgcccca ttaaatgagc aaatcgaaga actttcttca attgctggtt
     1741 ataaaggtgg tgagtttgag gaagatgaga ttattaagtt aactaatcaa gtcgcctata
     1801 tccaccaaat tctcaaaagc aatttagaag caattaagac cgaagcagaa aaggtaaatt
     1861 atattgtaga tgcgcttcct ttaggtgtta ttgttttaaa taataaaggc gaaatcaaac
     1921 aaattaatga atatggcaag aagttattta actatagtgc taatattact gaatatttgt
     1981 atttatcaag agttgaagag tttaataatt taattaaaga atctttagaa aagaaaacat
     2041 caatgcaagc agattgtctg cttgataata agatttataa tgtttcaact aaagttatta
     2101 ctaacgaatg gttaaatgaa ggtttagtag ttattgtaag tgatattgat ttagtaaaaa
     2161 gactagaaaa agctaaaaga gatttcttcg ctaatgcatc tcatgaatta aaatcaccac
     2221 ttacttcaat tattggtctg caagagatga ttgaaaataa cttcgtttct tcaaaagaag
     2281 aatttgaaga tattaacaaa agaacgattt ctgaggctag acgtagtatg aagttaatta
     2341 acgatatgct tagtttagct gaacttgaga gtttaaaagc taaaccaatg gatcaagaag
     2401 ttgatttaaa ggtattagtt ttagatgtat taaagagctt agatgtaaag atcaaagcta
     2461 agggttaaaa gtagaagttg atttagaaga tgctatttta aaaggatctt tagaagactt
     2521 taggaaactt gtagagaact taattgataa tgcaattaag tataataaag aacaaggttt
     2581 aattaaagtt gcaataaaag aaggaaaaga tgaaatagct ttatcagtag aagatactgg
     2641 tattggtatc aaacaaaaag aacaaaagaa aatttatgaa agattctatc gtgttgataa
     2701 aggtcgaagt caagaagttg aaggtaccgg tttaggttta gcaatcgtta agtatgtagt
     2761 attagccta
//
LOCUS       DAOSGS010000112         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_35689_length_2729_cov_4.347794, whole genome shotgun sequence.
ACCESSION   DAOSGS010000112 DAOSGS010000000
VERSION     DAOSGS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35689_length_2729_cov_4.347794"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLQ22_04310"
     CDS             <1..393
                     /locus_tag="PLQ22_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH59217.1"
                     /translation="GIGVIGVALASRDERLEVTSTDVNERALALAKENYELNGIKNYS
                     VFKSDGYENIKDSFDLIVLNPPIRAGKQIVDKLVLGAINYLNPNCYIWIVIRKSQGAP
                     SLVTRMEEVYGDVEIVKKKKDYWIIKAR"
     gene            552..>2729
                     /locus_tag="PLQ22_04315"
     CDS             552..>2729
                     /locus_tag="PLQ22_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH59218.1"
                     /translation="MSYKKVVYGKDRVRRDYARIQTNVEMPNLIEIQTKSFEWFLNEG
                     LKELFEEISPVTSQNEKLELQFGEYRFGEAKVSVQEAKRNKANYTKPLEVDFSLVFYE
                     DEPNPETGEKEVSRIANQSLFFGEFPVMTPSGTFIINGSERVIITQVIRSAGVYFAKE
                     VDKKTGAVLYSGQIIPTRGAWIEFETGKRDIWYVKFDRSNKLPLTTFLRALGLNSNRD
                     IINLFGMSEHLQNTFVKDTSFGSDDALRELYDKLRQGETATPDAARKYIASRIFENKR
                     YDLASVGRYKVNQKLDVFERLTAFGLNKVLLDQNIYREVNGKEELLYERGTELNKEIV
                     NFLKENRELIRRTFEYEIMLEGDSVTLEVLDLEIEIDGEMQKIRVLGNDQSETKNRIT
                     LSDIFASISYFMGLNIGLGKVDDIDHLGNRRLRPIGELLQNQARQGLMKLEKSIVSKM
                     STAKEFKTLVPDDLINGKTLLTTIQTFFATDQLSQFMDQVNPLSELTNKRRISALGSG
                     GLTRERAGFEVRDVHTSHYGRICPIETPEGQNIGLISSLATYSKVDKYGFIQTPYFKV
                     YQIGDKTYVSNDYRYYKADEEEEYYITQANVEMHKTIYQEKDLIKKDDRYFIEVEGKE
                     IGVFEAEYSGKPEYYTLDIVSKEVVARYRGENIEIEREMIELMDVSPKQIVSVSAALI
                     PFLEHDDANRALMGANMQRQAVPLLKPEAPIVATGIETQAAHDG"
BASE COUNT          995 a          353 c          535 g          846 t
ORIGIN      
        1 gggattggtg ttataggtgt tgctttagca agtagagatg aaaggctaga agttacatca
       61 actgatgtaa atgaaagggc cttagcctta gccaaagaaa actatgaact aaatggtatt
      121 aaaaactatt ctgtatttaa aagtgacggt tatgaaaata tcaaagatag ttttgattta
      181 atagttttga atccacctat aagagcagga aaacaaatag ttgataaact agttttgggt
      241 gcgattaatt acttgaatcc taattgctat atttggattg taattaggaa gagtcaagga
      301 gcgccatctt tagtaacgag gatggaagaa gtgtatgggg atgttgaaat agttaaaaag
      361 aaaaaagact attggattat aaaagcaaga taaaagagct aattaattag ctctttttta
      421 tttaaaaaga taatattttt cttaatttgc ccttgacttt aatcttcaaa tatattataa
      481 taattgttag tgttaataaa atgggcataa tgcgcccaat tagctaaata tatgggtgag
      541 gtgaaaaagt attgagctac aaaaaagtcg tttatggaaa agatcgtgta cgtagagact
      601 atgctagaat tcaaacaaac gtggaaatgc ctaatttgat tgaaattcaa actaagtcat
      661 tcgagtggtt tcttaacgaa ggcttgaaag agttgtttga agagattagt cctgttacgt
      721 cacaaaatga aaaattggag ttacaatttg gggagtacag atttggagag gccaaagttt
      781 ctgtacaaga agcaaaacgt aataaagcca attacacaaa gcctctagaa gttgattttt
      841 cattagtttt ttatgaagat gaaccaaatc cggaaactgg agaaaaagaa gtatctagga
      901 tcgcaaacca atcattattt tttggtgaat ttccggtaat gacaccatca ggaacattta
      961 ttattaatgg ttcggaaaga gtaattatta cacaagttat tcgttcagct ggtgtttatt
     1021 ttgcgaaaga agtagataag aagacgggcg ctgttttata ctcaggtcaa attatcccaa
     1081 caagaggagc atggattgag tttgaaacag gtaaaagaga catttggtat gtaaagtttg
     1141 accgttctaa taaactacct ctaacaacat ttttaagagc tttaggctta aactcaaata
     1201 gagatattat taatctattt gggatgagcg agcacttaca aaacacattt gtgaaagaca
     1261 catcatttgg tagcgatgat gcgttacgtg aattatatga taagttacgt caaggtgaaa
     1321 ctgctactcc tgatgctgca agaaagtata tcgcaagccg tatttttgaa aacaagcgtt
     1381 atgatcttgc tagtgtagga cgctataaag ttaatcaaaa actagatgtt tttgaaagat
     1441 taactgcatt tggtttaaat aaagttttat tagaccaaaa tatttatcgt gaagttaatg
     1501 gtaaagaaga attactatac gaaagaggaa ctgaattaaa caaagaaatc gttaatttct
     1561 taaaagaaaa ccgcgaattg attcgccgta cttttgaata cgaaattatg ttagaagggg
     1621 attcagttac tttagaagta ttagatttag aaattgaaat tgatggtgaa atgcaaaaga
     1681 ttcgtgtttt aggtaacgat caaagtgaaa ctaaaaacag aattacacta tcagatattt
     1741 ttgcatctat tagttatttt atgggtctaa acattggttt aggaaaagta gatgatattg
     1801 atcatttagg taatagaaga ttacgtccta ttggtgagtt gttacaaaat caagctcgtc
     1861 aaggattaat gaagttagaa aaatcaattg tttctaaaat gtctacagct aaagaattta
     1921 agactttagt tcctgatgat ttgattaatg gtaagacttt gcttacaaca atccaaactt
     1981 tctttgcaac tgaccaatta tctcagttca tggatcaagt taacccatta tctgaactta
     2041 caaacaaaag aagaatttct gctttaggct caggtggact tacaagagaa agagctggct
     2101 ttgaggtaag agacgttcat acatctcact atggccgtat ttgcccaatt gagactccag
     2161 aaggtcaaaa cattggttta attagctctc ttgctacata ttctaaagtt gataaatatg
     2221 ggtttattca aacaccttat tttaaggtat atcaaattgg tgataaaact tatgtatcta
     2281 acgattatcg ctattacaaa gccgatgaag aagaagaata ttacatcaca caagctaatg
     2341 tagaaatgca taaaactatt tatcaagaaa aagatttaat taagaaagat gatagatatt
     2401 ttattgaagt agagggtaaa gaaattggcg tatttgaagc tgagtattca ggaaagccag
     2461 aatactatac tttagatatt gtttctaaag aagtagttgc tcgttatcgc ggcgaaaata
     2521 ttgaaattga aagagaaatg attgaattaa tggacgttag tccaaaacaa atcgtttctg
     2581 tttctgctgc tttaattcca ttcttagagc atgatgatgc taatagagca ctaatgggtg
     2641 caaacatgca acgtcaagct gtacctttat taaagccaga agcaccaata gtagctactg
     2701 gtattgaaac acaagctgct catgatgga
//
LOCUS       DAOSGS010000113         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_35947_length_2715_cov_4.191353, whole genome shotgun sequence.
ACCESSION   DAOSGS010000113 DAOSGS010000000
VERSION     DAOSGS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35947_length_2715_cov_4.191353"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..589
                     /locus_tag="PLQ22_04320"
     CDS             32..589
                     /locus_tag="PLQ22_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH59219.1"
                     /translation="MARHFKKNPPKRTLKFVWCGSEEMGLLGSKAYVEKHKEELEKYE
                     LNINLDMTAVVLGYDIARCTTEMGLVNYINYLGKEVGFAIDASQGVYSSDSTPFADKG
                     VPALSFARIAPRGGAEIHSRKDDDKFLDKDNYYETCKFIIGFSERMANSVVMPVERSI
                     PENMRLELDYYLGIKERPEGPRRGF"
     gene            590..1990
                     /locus_tag="PLQ22_04325"
     CDS             590..1990
                     /locus_tag="PLQ22_04325"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH59220.1"
                     /translation="MQKSFSKYLKSKKPVMAALLERLQDKYEHVGILGTDTMASVCYV
                     DYHSSNVGEASDDCGFVIKVVHDGIYSEYATNELTMRRLDKIEEKIDELVKANMKTKK
                     VGLKLAEEEKLTQSFSRRNKGKYLSEEEIIAGLKETVDLFKQEEKIVHAGARFMVEEV
                     SKVYLSKNKDLEQYYFFPSAFIYSVAADQGKTQMFYNSVDDISMSKVLKELPKKVKET
                     ASVARELLVAENPEPGIYDVITAPSISGLIAHEAFGHGVEMDQFVKDRALGKEYIDKP
                     VASSLVSMRDGAASCFAIASYFFDDDGVLAGDTLVIDKGILKQGISDVNAASMLGTKP
                     TGNGRRQAYDHKTYTRMTNTFFEPGKDKLKDMIKSIEYGYLIDVTNNGMEDPKNWQIQ
                     CTATIGREIKNGKLTGKVVSPIIMSGYVIDLLKSISAVSDDFKINSGGSCGKGYKEWV
                     RVSDGGPYMKAKVKIG"
     gene            1987..>2715
                     /locus_tag="PLQ22_04330"
     CDS             1987..>2715
                     /locus_tag="PLQ22_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59221.1"
                     /translation="MIKRIEKILKSKGVDKYKIREINEEKYEAYYSLQKLEATRNTES
                     LTYEVTIYLPLGENQMLGSASFNLPYTMTVNQIAKKVDEAISSAQIAVSPYYELVEGV
                     GRKTFKDSFVLEKPLEEIDKVANIFFKETSENKKFNSLEVFYKQATIRLVNSNGVDYK
                     KISSVIEIEAIPSYDGAKKEDPSNQKVELYKYYKYPTLDKEKMQKDAKEALSDVEKRF
                     NAKKIDFKGKKNVILRDSDLNELVG"
BASE COUNT         1035 a          302 c          539 g          839 t
ORIGIN      
        1 ataatgcaac aggttctgct acatcttaga aattgcaaga cattttaaga aaaatccacc
       61 aaagagaact ttaaagtttg tatggtgtgg ttcagaagaa atgggattat taggatctaa
      121 ggcttatgta gaaaagcaca aggaagaact tgaaaaatat gagctaaata tcaacttgga
      181 tatgacagct gtagtattag gttatgatat tgctcgttgt actactgaaa tgggtttagt
      241 taactatatt aactatttag gtaaagaagt aggatttgca attgatgcaa gtcaaggagt
      301 atattcatca gattcaactc catttgcaga taaaggtgta cctgcgttaa gttttgctag
      361 gattgcacct cgtggaggag ctgaaatcca tagtagaaaa gatgatgata aattcttaga
      421 caaagataat tactatgaaa cttgtaagtt tattattgga ttttctgaga gaatggctaa
      481 ttcagttgta atgccagttg aaagatctat tcctgagaat atgcgtttag aacttgatta
      541 ttatttagga attaaagaaa gacctgaagg tccaagaaga ggattttaga tgcaaaaaag
      601 tttttcaaaa tatttgaaaa gtaaaaagcc agtaatggct gctttactag aaagactaca
      661 agataaatat gagcatgttg ggattttagg tacagacact atggcctctg tttgttatgt
      721 agactatcat agtagcaatg taggtgaagc tagtgatgac tgtggttttg taattaaagt
      781 tgttcacgat ggtatttatt ctgaatatgc tactaacgaa ctaacgatga gaaggttaga
      841 taaaattgaa gaaaagattg atgaacttgt aaaagctaat atgaaaacaa aaaaagttgg
      901 tttaaaatta gcagaagaag aaaaacttac tcagtctttt tcaagaagaa ataaaggtaa
      961 atacttaagc gaagaagaaa ttattgctgg actaaaagaa actgttgatt tatttaaaca
     1021 agaagagaaa attgttcatg caggtgctag atttatggtt gaagaagtat caaaagttta
     1081 cttaagcaaa aacaaagatt tagagcaata ctatttcttc ccaagtgcat tcatttatag
     1141 tgttgcagct gatcaaggta aaacacaaat gttttataat tcagttgatg atatttcaat
     1201 gagtaaagta ttaaaagaat tacctaaaaa ggttaaagaa actgctagtg ttgcaagaga
     1261 gttattagtt gctgaaaatc ctgagccagg tatttatgat gtcatcacag ctccttcaat
     1321 ttctggatta attgctcatg aagcatttgg tcatggtgtg gaaatggatc aatttgtaaa
     1381 ggatcgtgct ttaggaaaag agtatattga taaacctgtt gcatctagtc ttgtttcaat
     1441 gagagatgga gctgcaagtt gttttgcgat tgcttcatac ttctttgatg atgatggtgt
     1501 attagcaggt gatactttag taattgataa aggtatttta aaacaaggta tttctgatgt
     1561 gaatgctgca agtatgctag gaactaaacc tactggtaat ggtagaagac aagcatacga
     1621 tcataagaca tatacaagaa tgactaatac tttctttgag ccaggtaaag ataaacttaa
     1681 agatatgatt aagagtattg aatatggtta tttaattgat gttactaata acggaatgga
     1741 agatcctaaa aattggcaaa ttcaatgtac tgctacaatc ggtagagaaa tcaaaaatgg
     1801 taaactaact ggcaaagttg tatcaccaat tattatgagt ggttatgtaa ttgacttact
     1861 taaatcaatt tcagctgtat ctgatgactt taagattaac agtggtggat cttgtggcaa
     1921 aggatacaaa gaatgggtta gagtttcaga tggtggacca tatatgaaag ctaaggtgaa
     1981 aataggatga ttaaacgcat agaaaaaatc ttaaaatcta aaggtgtaga taaatataag
     2041 attagagaaa ttaatgaaga aaagtatgaa gcatactata gcttacaaaa gctagaagct
     2101 acaagaaata ctgaaagctt aacatacgaa gtaactattt atttaccatt aggtgaaaat
     2161 caaatgttag gttcagcatc atttaattta ccttatacta tgacagtaaa tcaaattgcc
     2221 aaaaaggtag atgaagctat ttcatcagct caaattgctg tgagtccata ttatgaactt
     2281 gtagaagggg ttggtagaaa gacatttaaa gatagttttg ttttagaaaa acctttagaa
     2341 gaaattgata aagtagctaa tattttcttt aaagaaacaa gtgaaaataa gaaatttaat
     2401 tctttagagg tattttataa acaagctacg attagattag taaactctaa tggtgttgac
     2461 tataagaaaa tatcttctgt tattgaaata gaagctattc cttcatatga tggtgcaaag
     2521 aaagaagacc catcaaatca aaaagtagag ctttataaat attataaata ccctacttta
     2581 gataaagaga agatgcaaaa agatgctaaa gaagctttaa gtgatgtaga gaaaagattt
     2641 aatgctaaga aaattgattt caaaggtaag aaaaatgtta tcttaagaga tagtgattta
     2701 aatgaattag ttggt
//
LOCUS       DAOSGS010000114         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_35982_length_2713_cov_8.413093, whole genome shotgun sequence.
ACCESSION   DAOSGS010000114 DAOSGS010000000
VERSION     DAOSGS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35982_length_2713_cov_8.413093"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            689..1135
                     /locus_tag="PLQ22_04335"
     CDS             689..1135
                     /locus_tag="PLQ22_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59222.1"
                     /translation="MKAKVKLALPGFAGNMDDMVIYYNSKLNKLITKRKGKSSYVPDY
                     NITKEIYAFARRINLSADYKKDCDKYINAYNSKNRRKGKAMSSWPSVWMKVMRAQKKL
                     LPDLDLNTLTREQAIEANLGCISIANAIEAGFLENVSGHQKLTSLI"
     gene            1259..2629
                     /locus_tag="PLQ22_04340"
     CDS             1259..2629
                     /locus_tag="PLQ22_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH59223.1"
                     /translation="MIKNSELISKLSNKQYYKTRNGKIFYNVSATIILKFINMFISFL
                     MVPITLDYLDKVRYGLWAALSSVLSWFFIFDIGIGNGLRNKYIELKAKDKISEARNYV
                     STAYALFGILILIIIIVFFVANHFISWYSLLKAPIEMQKELSDTVLVVFSIMCINFIV
                     KLINVILKAELKNALCDIFTVISHLISFLGIITLSHYTTPSLLKYAVLYTTSNVVVTV
                     IGSIVLYNGILRDVSPKINCVNFKLRKDILNVGIKFFIIQIMTILFFQTSSFFISVLI
                     GPEAVVGYNVTQKYFSIGSMIFYMLAEPLWSAYGDAYHKEDFSWIKSTFRRLLILYCI
                     VVFGLIIMIAVQKPFFKLWLKNKVEVDYILSGLFVVFYSLQMYTIIYNSFINATSKLR
                     LSMITSIFIVPAFIPAVFLFTKNLNLGSKGILLALIIVQTIPAAILTPIQCHKILAGS
                     KGIWQK"
BASE COUNT          922 a          386 c          439 g          966 t
ORIGIN      
        1 aagatgactt ccaagttacc tccctaaagg gtaatttcga agtcaatttt tttatctgcc
       61 ctgctttttg tctttccgta cttgttaagg aatctattcc ctattagaat ataatactct
      121 aacagctata tatttcaatg ccttacgcaa cacagagtgc ataagccacg gggcgaagct
      181 gtaagttgaa ctgccgaatt ctgatttttc aaaagatctt aaactaattt tcttagtttt
      241 aataaatgat tttaaccaat attacaaatt ggctttcaac taaatctgga aagagaagat
      301 agagtgtttg ttatagaaaa aataaccctc gcctacctag ctatcgtgca gataaagtgc
      361 agttaattgg cttttcccct aattgtgcca cggtagttgc accatatatt acttggtagt
      421 aaatggaaga tgctgaaatt taacttgcag attgtggttt tctttggtgc tctcaaaaaa
      481 agctacattt agataaaaaa tagtaatttt atttatgatt gtatctgtat gtgtatgtgg
      541 atgttaggaa attattctaa ctaattcata ctggtatcat aaaaatttct ctcatatttg
      601 tcaccacctt cccacttttg tcacccccta tataagtaga gggtcaaaaa aaactcacga
      661 cccttaataa aattatggga gtgacaaaat gaaagcaaaa gtaaaacttg ctttacccgg
      721 ttttgccgga aatatggatg atatggtaat ctactacaat tcaaaattga ataagttgat
      781 tactaaaaga aaggggaaat ctagttatgt acccgattac aatattacca aagaaattta
      841 tgcttttgcc cgtcggatta acttgtctgc tgattacaaa aaggattgtg acaagtatat
      901 caatgcttac aattccaaaa atcggcgtaa aggaaaagcg atgagttcct ggccctctgt
      961 ttggatgaaa gtgatgagag ctcaaaagaa acttttaccc gatcttgatt tgaataccct
     1021 aacccgcgaa caagcaattg aagccaattt gggttgtata tcaattgcta acgctataga
     1081 agcgggcttt ctggaaaatg tttcaggcca tcaaaagcta acaagcttga tttaaaaaat
     1141 tggggaggat aatctactga atttataatg cgaaaattta gaaattatcc tccaatttgc
     1201 tatagtaaag aatgtattag ttaaatctta ttatgaatag taatatatat aaataccaat
     1261 gataaaaaac tcagaattaa ttagcaaatt atcaaataag caatattata aaactcggaa
     1321 tggtaaaata ttttacaatg ttagtgccac tataattcta aagtttataa atatgtttat
     1381 aagttttttg atggtaccaa ttacacttga ttatcttgat aaagttcgct atggtttatg
     1441 ggctgctctt tcatcagtct tatcatggtt ttttattttt gatatcggaa ttggaaatgg
     1501 gttacgaaat aaatatattg agcttaaagc aaaagataag atttctgagg caagaaatta
     1561 tgttagtacg gcttatgcgt tatttgggat tctaattcta attataatta ttgtcttttt
     1621 tgtggccaac cactttataa gttggtatag tttgttaaaa gctcccatag agatgcaaaa
     1681 agaactatcc gatacagtat tagtggtctt tagcattatg tgcataaatt ttatcgtaaa
     1741 attaattaat gtaatactta aagcagagct taaaaatgct ctttgtgata tatttactgt
     1801 gatctcacat cttatttctt ttttaggaat aattacatta tcccattata caactccttc
     1861 attattaaaa tatgctgtat tatatacaac ttcgaatgta gtagtaacag tgataggatc
     1921 aatagtatta tataatggaa tattaagaga tgtttctcct aaaattaatt gtgttaattt
     1981 caaattaagg aaagatatct taaatgtagg cattaaattt tttatcatac aaattatgac
     2041 gatattgttt ttccaaacat caagtttttt tataagtgtt ctcattggac ctgaagcagt
     2101 ggttggatac aatgttacac aaaagtactt ttctataggt tcaatgatat tttatatgtt
     2161 agctgaacct ctttggagtg cttatggtga tgcttatcac aaagaggatt ttagttggat
     2221 taaatcaact tttcgtagat tattaatatt atattgtatt gttgtgtttg ggttgataat
     2281 tatgatagcg gtacaaaaac ctttcttcaa attgtggtta aaaaataaag tcgaggttga
     2341 ttatatattg tctggtctct ttgtagtttt ttactcttta cagatgtata caataatcta
     2401 caattcattc ataaatgcta cttcaaaact tcgtttaagt atgattacaa gtatttttat
     2461 tgtacctgcg tttatacctg ctgttttttt atttacaaaa aatttaaatt tgggatccaa
     2521 aggtatatta ttggctttaa taatcgttca gacaatacct gctgccatac taactcctat
     2581 acaatgtcat aaaatccttg ctgggagcaa aggtatatgg caaaaatgat attaagatat
     2641 agtaattatg gataacaaac aacgccttaa agctttaaaa aatgatctaa atttaattta
     2701 tggcaattat gca
//
LOCUS       DAOSGS010000115         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_36310_length_2696_cov_3.907989, whole genome shotgun sequence.
ACCESSION   DAOSGS010000115 DAOSGS010000000
VERSION     DAOSGS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36310_length_2696_cov_3.907989"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLQ22_04345"
     CDS             complement(<1..447)
                     /locus_tag="PLQ22_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59224.1"
                     /translation="MKSIEESNKNLESENNDTAVESPNVVVGKRLDLTVFEYIQNLGR
                     QIQTKSDNDLYLSKDSNITINVINTGVDIQLENLEKVSRSLISNNINSIVLVNNHKEK
                     RGYKLRKNDVVTIDLSSLYKILDQSIKNKDLNSGIYTVVSRKGDLDI"
     gene            complement(496..912)
                     /locus_tag="PLQ22_04350"
     CDS             complement(496..912)
                     /locus_tag="PLQ22_04350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOH59225.1"
                     /translation="MLRKLFNILTNKVFSFSALGVAILLISLHWLRFILFEQCVNNSG
                     IALGSLSGVMPIVLIVCIGTILAIVLFYAIKQKDIFLVILFIGGLSNWIDRVIDGFVC
                     DYLLFLDFYINFNDIVISVIVALIVFRDILKEKIAA"
     gene            complement(924..1736)
                     /locus_tag="PLQ22_04355"
     CDS             complement(924..1736)
                     /locus_tag="PLQ22_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59226.1"
                     /translation="MNFIDSFNNIFSVMPFLAWLGIIYLVVSYFFWREARILNKDRNS
                     IFDLWFVSFFGMAIWGRMSFVGANWSALYEGLPWSLLPYERYANNIYLFRLLPWKFIT
                     VWDGGFLFSGVAVAFIVISFAYIVLVKKWSWKQMMMPIITFSQYLLSLTLVVYGSLMR
                     YENAVILGLFLLLAVIIITIVRSLFSDKYFVVKYVFPIYTLLSELFIGYIFYSAQVAL
                     IDKINVGLLILVTIIMVIKYLTELLRPKTTIESISAVKPISPIVLNKPVKIS"
     gene            complement(1736..2530)
                     /locus_tag="PLQ22_04360"
     CDS             complement(1736..2530)
                     /locus_tag="PLQ22_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH59227.1"
                     /translation="MKIVALLFVVFWVFVVIWVWNDSGERSRNLFFRLTSVLTVLILN
                     IFGLVIYLLIRPQFTIEEVYWSDLERRYLKYETKELGDCPKCGYQLQPGYIYCPECNY
                     ELKVKCKGCELYFDKDNKYCPYCGLINKKEIPLEQISAKEMQEIASANKDEIVQTVEE
                     KGTRYSSKKGVFFKSADFFNKIFHTKTPEEKAEEKRLKEEKKAQEQRIKKEKRIEAEN
                     LKKAKLEAKKNNDKAVSESAEKKDQGSNQKKDKKVANKNNKKSNTK"
BASE COUNT          915 a          509 c          348 g          924 t
ORIGIN      
        1 tatatcaaga tcaccttttc ttgagacgac tgtatatatc ccgctattta gatctttatt
       61 ttttatggat tggtccagta ttttataaag actcgacaga tcgattgtta caacatcatt
      121 ttttctcaac ttataaccgc gtttttcttt atgattattg accagtacaa tactgtttat
      181 gttatttgaa atcagacttc ggctaacttt ttctaagttt tctaattgta tatctacccc
      241 agtattaata acattaattg tgatatttga atctttgctc aagtataaat cattatcact
      301 tttagtctgt atttgtcgac ctagattctg tatatattca aataccgtta aatctaatcg
      361 ttttccaacc actacatttg gactttctac agcagtatca ttattttcag attcaagatt
      421 tttattactt tcttcgatgc ttttcataat cgttgattaa aaattcataa ctacaaactt
      481 acatatcaca tatcattaag ccgctatctt ttctttaagt atatctctaa atacaatcaa
      541 agcaacaata actgatataa ctatatcatt gaagttgata tagaagtcta aaaaaagtag
      601 ataatcacaa acaaatccat ctatcaccct atctatccaa tttgataatc caccaataaa
      661 gagaataaca aggaaaatat ccttttgttt tatcgcataa aaaagcacaa ttgcaagaat
      721 agtgcctata caaacaataa ggacaatcgg cattactcca cttaacgacc ctaaagctat
      781 accactatta tttacacact gttcaaagag tataaagcgt aaccaatgaa gtgaaatcaa
      841 caaaatcgca actcccaaag cagaaaatga aaagacttta tttgtcagga tattaaatag
      901 ttttctaagc ataattgtag agattagctt attttgacag gcttattaag tacaatagga
      961 gaaatgggct ttaccgcaga gatagattcg attgtagttt taggtctgag aagctcagtt
     1021 aaatacttta tcaccattat tatcgtgacc aatattagta gacctacatt tatcttatcg
     1081 attagagcca cctgagcaga atagaaaatg taaccaataa aaagctcaga cagtaaagta
     1141 taaattggga aaacatattt aaccacgaaa tatttatccg aaaaaagtga ccttacaatt
     1201 gtaataatta taactgccag caacaaaaat aaaccgagta ttacagcatt ttcatatctc
     1261 attaatgaac catatacaac aagagttaaa gagagtaagt attgtgaaaa agtaataatt
     1321 ggcatcatca tctgcttcca actccacttt ttaaccagga caatatatgc aaagcttatt
     1381 actataaatg caacagctac tcctgaaaat aaaaatccac catcccaaac tgtgataaat
     1441 ttccaaggca ataatctaaa cagatatatg ttattcgcat acctttcata aggtaataaa
     1501 gaccatggga ggccttcata cagtgcagac cagttagcac ctacgaaaga catcctaccc
     1561 catattgcca taccaaagaa actgacaaac catagatcaa atattgaatt tctgtcttta
     1621 ttgagaatcc ttgcttcacg ccagaaaaaa tatgacacta cgagatatat tatccccagc
     1681 catgctaaaa aaggcataac gctaaaaata ttgttgaacg aatctataaa gttcattatt
     1741 tagtattgct ttttttatta tttttattag ctaccttttt atctttcttc tgattactac
     1801 cttgatcttt tttctctgca ctttcagaaa ctgctttatc gttattcttt ttagcttcaa
     1861 gtttggcctt cttcaaattc tcagcttcga ttcttttctc tttcttaatt ctttgttcct
     1921 gagccttttt ctcttccttt aatctttttt cttctgcttt ctcttctggt gtttttgtgt
     1981 gaaagatttt attgaagaaa tctgcgcttt tgaagaatac accttttttg cttgaatacc
     2041 tcgtaccttt ttcttcaaca gtctgaacaa tctcatcctt attagcactt gcaatttctt
     2101 gcatttcttt tgcagaaatc tgttcgagtg ggatttcttt cttatttatc aaaccacaat
     2161 aaggacaata cttattatcc ttatcaaaat acaattcaca acctttacat tttaccttta
     2221 attcgtaatt acattcagga caataaatat atcctggttg taattgatat ccacactttg
     2281 gacaatcacc aagttctttt gtttcgtatt taagatatct tctttcaaga tcagaccaat
     2341 acacttcctc aatagtaaac tgaggcctga taagcagata tataactaac ccaaaaatat
     2401 taagtatcaa aacggttaag acactagtca gtctaaagaa gagatttcga gatctctcgc
     2461 ctgaatcatt ccaaacccat attacaacaa agacccaaaa tacaacaaaa agaagtgcaa
     2521 ctattttcat tacaccatca aagtttattg taccgatatt gttgagggta tcgaggatat
     2581 attgttctac gctcattttg actgaactta aatttattta tttttgtgga atacacaaaa
     2641 gcaaatgcag aaggagggag ttgaaccctc atcctcttgc gaggacggga ttttga
//
LOCUS       DAOSGS010000116         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_37633_length_2632_cov_3.858362, whole genome shotgun sequence.
ACCESSION   DAOSGS010000116 DAOSGS010000000
VERSION     DAOSGS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37633_length_2632_cov_3.858362"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /gene="leuS"
                     /locus_tag="PLQ22_04365"
     CDS             <1..1684
                     /gene="leuS"
                     /locus_tag="PLQ22_04365"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOH59228.1"
                     /translation="TVFTTRADTLFGATYCVFAPEHPLIKKLVPSRYKKQVEEYQALA
                     KAKSDLERTDLNKDKTGVFTGLYAINPVNNKEIPIWIADYVLINYGTGAIMAVPGHDQ
                     RDYEFAKKFKLDIIPVLDGDISEQAYEGDALHINSGFLDGLNKEDAIRRMLEYLEAKG
                     IGKKEVNYKLRDWIFSRQRYWGEPFPVVFLEDGTIEILDENELPLELPKLKKITPSKT
                     GESPLVNATEWLHVMTKDGLKGKRDTNTMPQWAGSCWYFLAYALKEEGGYIPLSSEKA
                     KKILNYWLPVDLYIGGTEHAVGHLLYSRFWYKVLHDVGLLETDEPFLKLFNQGMILGS
                     DGEKMSKSKGNVVNPDDIIEEFGADSLRLYEMFMGPLEATKPWSTQNIEGPRRFLERV
                     YRLYKEVYKPAKENKNLEKIYHQTVKKVTEDYENLRFNTAISQMMIFINECYKDPNIP
                     NEYMEGFLKLLAPVCPHITEELNETVLKSNVLLTNQKWPTYDEAKTVENLVTYVFQVN
                     GKIRDKLEVNVDTTKEEIESLALASENIQKYIDGKPIRRIIVVSGKLVNIVV"
     gene            1686..2456
                     /locus_tag="PLQ22_04370"
     CDS             1686..2456
                     /locus_tag="PLQ22_04370"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH59229.1"
                     /translation="MLPEFIKDFFIDSYTLLAIIGVLSLILYVILYLEKKLNYSRKRV
                     NKLLVIGAISGVITVVFALLFDLLVHYLKSGEVRFGVTFLGGVIPGLICFTLLNYYFN
                     KEERANLKEILGVLVRGIILGHMFGRIGCFLAGCCYGVPTNSVFGVIYPDIPYSPRGE
                     IFAKYGEGVAVHPTQIYEALFLLSLFLVLHFVPKLRENSFVIYLFSYGIFRFFIEFIR
                     GDDRGSLIPFLSPSQILSFILIVMGAVVLLYRYRKKGV"
BASE COUNT          936 a          290 c          500 g          906 t
ORIGIN      
        1 tactgttttt actactagag ctgatacttt atttggagca acttattgtg ttttcgcacc
       61 tgagcatcca ttaattaaaa aactagttcc aagtagatat aaaaaacaag tagaagaata
      121 tcaagcatta gctaaggcta aatctgatct agaaagaaca gatttaaata aagataaaac
      181 tggagtattt acaggtttat atgcaattaa tccagttaat aataaagaaa ttccaatttg
      241 gattgctgac tatgtattaa taaattatgg aacaggggca attatggcag ttcctggtca
      301 tgatcaaaga gactatgagt ttgcaaagaa atttaagtta gatattattc ctgttttaga
      361 cggtgatatt agtgaacagg cttatgaagg agatgctttg catattaact caggcttttt
      421 agatggctta aataaggaag atgcaattag aagaatgctt gaatatttag aagctaaagg
      481 aattggtaaa aaggaagtta actataaact aagagattgg atttttagtc gccaaagata
      541 ttggggagaa cctttccctg ttgtgttttt agaagatgga acaattgaaa ttttagatga
      601 aaatgaatta cctttagaat taccaaaact taagaaaatt acaccttcaa aaactggaga
      661 atcaccttta gttaatgcaa ctgaatggtt acatgtaatg actaaagatg gcttaaaagg
      721 taaaagggat actaatacaa tgcctcaatg ggcagggtct tgttggtact tcttagctta
      781 tgctttaaaa gaagaaggag gttatattcc tctaagtagc gaaaaagcca agaagatttt
      841 aaattactgg ttaccagttg atttatatat tggtggtact gaacacgcag ttggtcactt
      901 actttattct cgcttttggt ataaagtatt acatgatgtt ggtttactgg aaactgatga
      961 accattctta aagttattca atcaagggat gattttaggt agtgatggtg agaaaatgag
     1021 taagtcaaaa ggtaatgtag ttaacccaga tgatattatt gaagaatttg gtgctgactc
     1081 tttaaggcta tatgaaatgt ttatggggcc gttagaagca actaaacctt ggtcaacaca
     1141 aaatattgaa ggaccaagaa gatttttaga aagagtttat cgtttatata aagaagtata
     1201 taagccagcc aaagaaaata agaatttaga aaagatttat caccaaacag ttaagaaagt
     1261 tactgaagat tatgaaaatt taagatttaa tacagctatt agtcaaatga tgatttttat
     1321 taatgagtgt tataaagatc caaatattcc aaatgaatat atggaagggt tcttaaaact
     1381 tttagctcca gtatgtcctc atattactga agagttaaat gagacagttt taaaaagtaa
     1441 cgttttactt actaatcaaa aatggccaac ttatgatgaa gcaaaaacag tagagaattt
     1501 agtaacttat gttttccaag ttaatggtaa gattagagat aagctagagg ttaatgttga
     1561 cactactaaa gaagaaattg aaagtttagc tttagcttca gaaaatatcc aaaaatatat
     1621 tgatggtaaa cctataagaa ggattatcgt agtttctgga aagttagtaa atatcgtagt
     1681 ataaaatgtt accagaattt attaaagact tctttattga tagttatact ttacttgcaa
     1741 ttattggagt tttatcgtta atcttatatg ttattcttta tttagaaaag aaactaaatt
     1801 attcaagaaa gagagtaaac aagcttttag taataggagc tatttctgga gtgataactg
     1861 tagtctttgc tttgcttttt gatttgttag ttcattattt aaaaagtgga gaagtaagat
     1921 ttggtgttac tttcttaggt ggagttattc caggattgat ttgttttact ttacttaatt
     1981 attactttaa taaagaggaa agagcaaact taaaagagat tttaggagtc ttagttaggg
     2041 gaataatttt aggtcacatg tttggaagaa ttggttgctt tctagcagga tgttgttatg
     2101 gcgtgccaac taattcagta tttggtgtta tttatcccga cattccttat tcaccaagag
     2161 gagaaatctt cgcaaagtat ggtgaaggag tagctgttca tccgacacaa atttatgaag
     2221 ctttgttttt acttagtttg tttttggttt tacattttgt accgaaatta agagaaaata
     2281 gttttgtaat ttatttgttt agttatggta tatttagatt ttttatcgag tttattagag
     2341 gtgatgatag aggaagttta attccttttc ttagtccatc acaaatacta tcatttattt
     2401 tgatagttat gggtgcagtt gttttattgt accgataccg caaaaaagga gtttaaaatg
     2461 aaaaagcata tttggttagt cttattgatt ttaatatttg ctttaagtgg gtgtaataaa
     2521 gttgatttta ctcttactaa aacgacgtat gaattatacg taaatgaaag tgctgattta
     2581 gaatatcaaa caaagacgag taatctaaaa atagaaatta aaattgaaga tg
//
LOCUS       DAOSGS010000117         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_38208_length_2607_cov_5.757053, whole genome shotgun sequence.
ACCESSION   DAOSGS010000117 DAOSGS010000000
VERSION     DAOSGS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38208_length_2607_cov_5.757053"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1760)
                     /gene="brxL"
                     /locus_tag="PLQ22_04375"
     CDS             complement(327..1760)
                     /gene="brxL"
                     /locus_tag="PLQ22_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HOH59230.1"
                     /translation="MKTLDEKIKQEFPEESVIKIPQNYSVFAGKNLPSFIKDFLIKRY
                     TDSYGNLDRNGILKFFSDHIPSKNNNLKNRLRTHREEVTVLARLIIETDLKNDRLLFS
                     IPDLGIKPSEGRIPDHVAKKNKELKDGELWGVITLIYQPPADREKGFIELVNYKAFKP
                     YKVDLDYFCSARREFTTLEWIDLLIRSMEYNPEGFDSISQKLMFLSRLLIFVEPRLNL
                     IELAPKGTGKSYVFGNLSKYGWLISGGVVSRAKLFYDMQRKSMGMITLYDFITLDEIQ
                     TIKFTDSAELRAALKSFLEQGYTNIMGTRVESLAGFLIMGNISLSIENKPVSKKYFSE
                     LPDTFKESALLERFHGFIEGWRLPRFNTSLLVHGHTLNVEYFSEILHELRNNYLYATI
                     VDDLIDIPPKSDGRHVIAIKRLATAYLKLLFPNITNTEDVNKEDFNNFCLQPAIEKRK
                     IILDQLRLIDPEYSQEKYNIPDFKLSR"
     gene            complement(1757..>2607)
                     /gene="pglZ"
                     /locus_tag="PLQ22_04380"
     CDS             complement(1757..>2607)
                     /gene="pglZ"
                     /locus_tag="PLQ22_04380"
                     /inference="COORDINATES: protein motif:HMM:NF033445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-4 system phosphatase PglZ"
                     /protein_id="HOH59231.1"
                     /translation="DKVLWIDAIGIEWVSFIENYIINQKHDLNVINKFIGVANLPTST
                     DQNKFPDVKYIQDFDNHIHSTVYSFPNSIIKQFDEIKRIIDTYIILDSEQTIAIVSDH
                     GLTALSRLAVSKRYGKDDSHEGRFIEVIDKYHISDSDYIIHKSDIDQKNYLIALKHGS
                     LGKKPIREVHGGVTPEEVLVPFIVVSNKKQSAEIDYSIILDKTEIPKKEPLISINITP
                     KPSSANIYLKGKIIKLIFNEKTLKWEINLDKSLSGKIPIKVKTGKSEKAFTINIISGI
                     IEEDLF"
BASE COUNT          869 a          425 c          361 g          952 t
ORIGIN      
        1 tacttcagaa taattcattt tgaaatatat tgactgcaaa ggtactataa atattggtaa
       61 tattgcaaac gaaatatact atttcttgca aaattgaatt attctctgaa atatttacgg
      121 atataaatgc aaattattca tgattaattt caattcattc atttcattat cataaaatta
      181 accaaaatgg cccgacaata tcggaaaagt gtgttgcccg acacatctaa agcaaagagt
      241 atcacgacta tagattaaca tcaagacata tttttattaa aaaagaggta taacatattt
      301 taacaatcaa ctgatgacta tttttgtcat ctactaagtt taaaatcagg aatattatat
      361 ttttcttgag aatattctgg atcaataagc cttagctggt ctaagataat ttttcgtttt
      421 tcaatcgcag gttgtaaaca gaaattatta aaatcttcct tattaacatc ctcagtatta
      481 gttatattcg gaaatagtaa ctttaaatat gccgtagcca atcgtttgat tgcaataacg
      541 tgtcttccat cagatttagg tgggatatca attaaatcat caacaatagt agcatacaaa
      601 taattatttc taagctcgtg caaaatttca gaaaaatact cgacatttaa tgtgtgacca
      661 tgcactaata agctggtatt aaacctaggt aaacgccagc cttcaataaa tccatgaaat
      721 ctttctaata atgctgattc tttaaaggtg tctggtaatt ccgaaaaata ttttttacta
      781 acaggtttat tttctattga taatgaaata ttacccataa taagaaatcc ggctaaggac
      841 tctacacgtg tacccataat atttgtataa ccttgttcta ggaaactttt caaagcagct
      901 cttaattcag cagagtctgt aaacttaatg gtttgaattt catcgagagt aataaagtca
      961 tacaaagtaa tcatgcccat tgattttctt tgcatatcat aaaacaactt agctctggaa
     1021 actacaccac cactaataag ccaaccatat ttactaaggt taccaaacac atacgattta
     1081 cctgtccctt ttggagctaa ttcaattaga tttaacctcg gttcaacaaa tattaataat
     1141 cgacttagga acattaactt ctgagagatg gagtcgaatc cctctggatt atactccatt
     1201 gatcttatta ataagtcaat ccattctaaa gttgtgaatt ctctacgagc agaacaaaag
     1261 taatctagat ctactttata tggtttgaaa gctttgtaat taacaagttc aataaatccc
     1321 ttttctctat ctgctggtgg ttggtatatc aaagttataa ccccccacag ttcaccatct
     1381 tttaactctt tatttttttt tgctacgtga tcaggtattc ttccttcact aggttttatt
     1441 cccaaatcag gaattgaaaa gagcaatctg tcattcttca agtcagtttc aataattaat
     1501 ctcgctaaaa ctgtaacttc ttctctgtga gtacgcaaac gattttttag attattgttc
     1561 ttacttggaa tgtgatcact gaaaaattta aggataccat tgcgatctaa gttaccatag
     1621 ctatcagtat agcgtttgat taaaaagtcc ttaatgaatg aaggcaggtt tttaccagca
     1681 aaaacgctat agttttgagg aatcttaata accgattctt cgggaaattc ttgttttatt
     1741 ttttcgtcaa gtgttttcaa aataaatcct cctctattat acctgagatt atgttaattg
     1801 taaatgcctt ttcactttta cctgttttta ctttaatagg aattttgcct gataaagatt
     1861 tatcaagatt tatttcccac tttagagttt tttcattaaa aataagttta attatttttc
     1921 cctttaaata aatatttgct gaagatggtt ttggagttat attaattgaa ataagaggtt
     1981 cttttttagg tatctctgtc ttatcaagta taatagaata atctatttct gcagattgtt
     2041 tcttgttaga aacgacaata aatggaacta gcacttcttc aggagttact cctccatgaa
     2101 cttctcttat tggtttttta cctagtgaac catgctttaa tgcaattaaa tagttttttt
     2161 ggtcaatatc tgatttatga attatgtaat cagaatctga gatatggtat ttatctatca
     2221 cttcaataaa acggccctca tgactatcgt cttttccata tcttttagat actgcaagtc
     2281 tggataaagc agttaatcca tgatcagaaa ctattgctat tgtttgttct gaatcaagaa
     2341 ttatgtaagt atcaattatt cttttaattt catcaaattg tttaatgatt gagttcggaa
     2401 aagaatatac agttgagtgg atatgattat caaaatcttg aatatacttg acgtcaggaa
     2461 atttattttg gtcggtactt gttggcaaat tggcaacacc aataaacttg ttaattacat
     2521 ttaaatcatg cttttgatta ataatataat tctcaataaa agacacccat tcaatcccta
     2581 tggcatcaat ccataatact ttatcca
//
LOCUS       DAOSGS010000118         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_38705_length_2583_cov_4.135680, whole genome shotgun sequence.
ACCESSION   DAOSGS010000118 DAOSGS010000000
VERSION     DAOSGS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38705_length_2583_cov_4.135680"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /gene="rpsI"
                     /locus_tag="PLQ22_04385"
     CDS             <1..380
                     /gene="rpsI"
                     /locus_tag="PLQ22_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH59232.1"
                     /translation="YEGIGRRKTSTARVRVFEGASASSVNLKPVEEYFALKTLVGKTM
                     RPFVVTDQIGKLYFTAKVTGGGINGQADAVKLGVARALVKMDPLLKTVLRKEDLLIRD
                     PRMVERKKYHHIKARKKPQFSKR"
     gene            complement(349..2292)
                     /locus_tag="PLQ22_04390"
     CDS             complement(349..2292)
                     /locus_tag="PLQ22_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH59233.1"
                     /translation="MKKVKTAIVYYKYYSHGNICTVEISTAICRGFPSTKIIGMKPKQ
                     ARVIIEKINIALKSAGYKLPQEKTRIYLKYYKGNNSDQLIPSNLSLELPIAISILLCS
                     NQINFVSESKVINDNITDSIPKVDLNNNSLATNSYSVLAGGDLSFDSSISGYTEILPY
                     IKFFDKKRRIVLTKSRKPEITKSTHNIFVVLPDKCRKLVLYLQDQGYEIKNNCNLRFI
                     FIGSLANLQQSINLILNKEEANRDSLFHSNPLLVNENNSGQAKPYRIEKTVSNAETKS
                     KSESRLQLPTKITIPTDKMSYLSKPQNNYISKENLTTLNYLVGQESAKRACIISAGGG
                     HHILFIGPIGSSKTLLAHSISEITPSLSLYEILDMARYHRVKIEEIEQYLQKIDTANK
                     NLQNARIFELSRPIIEIMHNTTIPGLLGNKNNPGLLAKADKGFLLLDEISEISSSVLN
                     SLKSSLDYRQIKVGANDIIPTRYTLIATSNTCPCGKDAKDCKCKLGEKINYIKKIPES
                     LINRIDLLSRVSVTDFSTISSAQNSLSGHINNVKDSTDYRNIVKKVREIQNLRYHNED
                     FSTNAEITLDKINTYCECTSSATKILHTSIDKLGLNGRDYIKILKIARTIADLDSKEI
                     ITEENIYEALSYRTTSLRTEVSF"
BASE COUNT          804 a          375 c          423 g          981 t
ORIGIN      
        1 attatgaagg tataggtcga agaaagactt ctacagcaag agttagagta tttgagggtg
       61 ctagtgcatc atcagttaac cttaaaccag ttgaagaata tttcgcattg aagacattag
      121 taggtaagac aatgagacct tttgttgtta cagatcaaat tggtaaacta tattttactg
      181 caaaagtaac tggtggagga atcaatggtc aagcagatgc agtcaaattg ggcgttgcaa
      241 gagcacttgt taaaatggat ccattactta aaacagtatt acgtaaagaa gatttactaa
      301 ttagagatcc tcgtatggtt gagcgtaaaa aatatcacca tattaaagct agaaagaaac
      361 ctcagttctc aaagaggtag ttctgtaaga cagagcttcg tatatatttt cttcagttat
      421 tatttcttta gaatctaagt cggcgattgt tcgtgctatc tttaaaatct ttatataatc
      481 tcggccattt aatcccagtt tatctattga ggtatgtaaa attttcgtgg ctgatgatgt
      541 acactcacaa tatgtattga ttttgtctag tgttatctct gcattagtac tgaaatcctc
      601 attatgatat cgaagatttt ggatttctcg gacttttttt actatatttc tataatcagt
      661 tgaatctttt acattattta tatgaccaga taaactattc tgcgctgaag atattgtact
      721 aaaatcggta acacttactc gagaaagcaa atcaatccga ttaattagtg attccggtat
      781 cttttttatg taatttattt tctctccaag cttacatttg caatcctttg catctttacc
      841 acaaggacaa gtgttagaag tcgcaataag tgtatatcgt gttggtatta tatcgtttgc
      901 tcctactttt atctgtctat agtctaaaga tgattttaat gagtttaata ccgagctact
      961 tatctccgat atttcatcaa gtaacaaaaa gcctttatct gcttttgcaa gtaaaccagg
     1021 attattctta ttaccgagaa gacctggtat tgtagtgtta tgcattattt ctataattgg
     1081 tctgcttaat tcgaatattc gagcattctg gagattttta tttgcagtat ctatcttttg
     1141 caagtattgt tctatctctt cgattttaac tctatggtac cttgccatat caagaatttc
     1201 atacaatgat aacgatgggg tgatttctga aatagagtgg gctaataatg ttttacttga
     1261 tccgatagga ccgataaaga gtatatgatg cccaccgcct gcagaaataa tacatgctct
     1321 ttttgcactc tcttgtccta ctaagtaatt tagtgttgtt aaattttctt ttgaaatata
     1381 attattctgt ggcttgctta aatatgacat cttgtcagtc ggaatagtaa tttttgtagg
     1441 tagctgcaat cgagattctg atttactttt cgtttctgcg tttgatacgg ttttctcaat
     1501 cctgtaaggt tttgcttgtc ctgaattgtt ttcatttact aacagtggat tagagtgaaa
     1561 aagtgaatct cgattagctt cttctttatt aagaataaga ttaatcgatt gttgcagatt
     1621 tgcaagcgaa ccaataaaaa taaaccttaa attacaatta tttttgattt cgtagccttg
     1681 atcttgtaaa taaagaacta attttctgca cttgtcaggt aataccacaa aaatattatg
     1741 agttgatttt gttatctcgg gttttctact tttcgttaga acgattcttc tttttttatc
     1801 aaagaacttt atgtagggca gtatttctgt ataacctgag attgaactat caaaacttag
     1861 atcgcctcca gcgagaacag aataactatt tgtagctaaa ctattattat ttaagtcaac
     1921 ttttggaatt gagtcggtaa tgttatcgtt gataacttta gactcggaga caaaatttat
     1981 ctgatttgaa caaagcaaaa tactgattgc gattggtaat tcaagagaaa gattagaggg
     2041 tattaactga tctgaattgt ttcctttgta atactttaga taaattctgg ttttttcttg
     2101 aggtaattta tatcctgcag attttaaagc aatatttatt ttttcaatta ttactcgagc
     2161 ttgtttaggc ttcatgccaa taattttggt acttggaaaa ccacggcata ttgctgtcga
     2221 tatttctact gtgcagatat ttccatgaga ataatattta taatatacga ttgccgtttt
     2281 tacttttttc attatctcaa ttttagcggt gtattattaa atcactctta aatatttatg
     2341 aaatcgttga ataaattaaa ccatacattt cttatttttt ttgataaata atattgataa
     2401 ttgtgtattt tcacataata aatacatata aatacttgct ttaaagaata aactaaactt
     2461 actaaatagt gaaatataag agggcgatca gtaattatat aaaattctta ctgtattgtt
     2521 gctgattata tttgacttat tgcataaaaa gtgattatct ataatcaagc aaatataaat
     2581 ttt
//
LOCUS       DAOSGS010000119         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_39412_length_2552_cov_3.130156, whole genome shotgun sequence.
ACCESSION   DAOSGS010000119 DAOSGS010000000
VERSION     DAOSGS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39412_length_2552_cov_3.130156"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLQ22_04395"
     CDS             <1..840
                     /locus_tag="PLQ22_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH59234.1"
                     /translation="IEHGSSIYKRDLINDDHKLSRTFASFVERFYPKFPLGENYRGPA
                     GGLFTSMEDLAKILIVLLNEGKYKDIRILKKETVEFMSQFHWFGNPEDSSYKAKGLSL
                     KLTTDYGPRILRGHTGSAYGVISCMFYSLEQKVGICFITNGGHITYNIDNKADLLTEI
                     LKEFTKTFICGEQKHTFVIDLLGKESKLDNRTIYMPTKVVKECCEYYVPALTIAEGLL
                     EIPRMTENYIEIEKNKISQILSLWGGAVKEVDGFLLLPVKKVLNSLRLEYQIDENKIV
                     ITY"
     gene            849..1142
                     /locus_tag="PLQ22_04400"
     CDS             849..1142
                     /locus_tag="PLQ22_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59235.1"
                     /translation="MIKRQVVKKKQKYSNNSIFFMVMAVVMGLVESFLLALIINNSVI
                     LKKYDSYPEGFDRVPKNFFVFSIVIAVILMALFGFTLYLTLKLLFKRKQEGTL"
     gene            1142..2296
                     /locus_tag="PLQ22_04405"
     CDS             1142..2296
                     /locus_tag="PLQ22_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOH59236.1"
                     /translation="MSHFDKVVDRRGTSCFKWDTVEEGVYSFTIADSDYEVPKEVKEA
                     LLKRVEHGIFGYTVVPNSYYEAVINWCMKRYGFNIEKDDIIIMNKVLESISLLINLYT
                     KEGDKIVINPPVYPPFFALTEGNKRVLSSNHLINDNEHYSLDLEDLENKFKEGAKMYI
                     LCNPHNPVGKIYTKEEIEAIVRLCNKYDVFLVSDEIHSDLIIYGHEFTSVGKFFHLHD
                     KIAVCISPSKTFNIAGLRIANLVLKNEEIKANVRKHLDRYKIGGASLLSLLACETAYN
                     KCEYWVIEQQKHLYKNYEFARNYLNKEVPYLKFANPHSTYLLWINLQYLNKTSEQIIK
                     ELKEYKLAVNSGCDYCKDYDGYIRFNLACPQSILKEGLKRLVKYLKDQEK"
     gene            2369..>2552
                     /locus_tag="PLQ22_04410"
     CDS             2369..>2552
                     /locus_tag="PLQ22_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59237.1"
                     /translation="MKAVLLLHGFLSNKYDFKNLIPILEKYYDHIEVITFPGHSVDES
                     YDNFTLGNTLELLFSTY"
BASE COUNT          950 a          258 c          447 g          897 t
ORIGIN      
        1 attgaacatg gatcaagtat ttataaaaga gacttaatta atgatgatca taaattaagt
       61 cgaacttttg catcgtttgt ggaaagattt tatcctaagt ttccacttgg ggaaaattat
      121 cgtggacctg ctggtgggtt atttactagt atggaagact tagctaagat tttaatagtt
      181 ttactaaatg aaggcaaata caaagatata agaatattaa aaaaagaaac cgttgagttt
      241 atgtctcaat ttcattggtt tgggaatcct gaagattcaa gttacaaggc aaaaggatta
      301 tccttaaaac ttactactga ttatggacca agaattttac gaggtcatac gggatctgct
      361 tatggtgtta ttagctgtat gttttacagt ttagagcaaa aagttggaat ttgttttatt
      421 actaatgggg gacatattac atataacatt gataataaag ctgatttact tacagaaata
      481 cttaaagaat ttacgaagac ttttatttgt ggtgaacaaa aacatacctt tgttattgat
      541 ttattaggca aagaaagtaa attagataat cgaactattt atatgccgac taaagttgtt
      601 aaggaatgtt gtgaatatta tgttcctgcc ctaacaattg cagaagggtt attagaaatt
      661 ccaaggatga ctgaaaatta tatagaaatt gaaaaaaata aaattagtca gattttaagt
      721 ctatggggtg gtgcagtaaa agaagttgat ggctttttat tactaccagt taaaaaagtt
      781 ttgaatagtt taagattaga atatcaaatt gatgaaaata aaatagttat tacttattag
      841 gaggaaatat gataaaaaga caagtagtga aaaagaaaca aaaatattct aacaatagta
      901 ttttctttat ggtaatggca gttgtaatgg gattagttga atcattttta cttgccctta
      961 ttattaataa tagtgttatt ttaaaaaagt acgatagtta tcctgaagga tttgatagag
     1021 ttccaaaaaa tttctttgtt ttctcaatag tgatagcagt gattttgatg gcattatttg
     1081 gttttacact ttatctaaca cttaagttat tatttaaaag aaagcaagaa ggtacattat
     1141 aatgagtcat tttgataagg tagtagatag aagagggaca agttgtttta agtgggatac
     1201 agtagaggaa ggtgtttatt ctttcacaat cgctgattca gattatgaag tgccaaaaga
     1261 agttaaagaa gctttattaa aaagagtaga gcatggtatc tttggttata ctgttgttcc
     1321 aaatagttat tatgaagctg taattaattg gtgcatgaaa agatatggct ttaatattga
     1381 aaaagatgat attattatta tgaataaagt attagagtca atttctttat taataaattt
     1441 atatactaaa gaaggggata aaattgtgat taatcctccg gtatatccac cattctttgc
     1501 tttaactgaa ggaaataaaa gagttttatc ctcaaatcat ttaattaatg ataatgaaca
     1561 ttatagttta gatttagaag atttagagaa taaatttaaa gagggagcta aaatgtatat
     1621 tttatgcaat cctcataatc cagttggtaa aatctataca aaagaagaaa ttgaagctat
     1681 tgttagactc tgtaataaat acgatgtatt tttagtatca gatgaaatcc attctgactt
     1741 aattatttat ggtcatgagt ttacatcggt aggtaagttt tttcatttac atgataaaat
     1801 tgctgtttgt atttctccta gtaaaacatt taatattgct ggtctaagaa ttgcaaattt
     1861 ggttttaaaa aatgaagaaa tcaaggctaa tgttcgtaag cacttagatc gctataagat
     1921 aggtggagca agtcttttaa gtttacttgc ttgtgagact gcttataata aatgtgaata
     1981 ttgggttatt gaacagcaaa agcatttata taaaaattat gagtttgcaa gaaattattt
     2041 aaacaaagag gtaccttatt taaaatttgc taatccgcat agtacttact tactttggat
     2101 taatttgcaa tatttaaata aaactagcga gcaaataatt aaagagttaa aagaatataa
     2161 acttgcagtt aattctggtt gtgattactg taaggattat gatggctaca ttcgttttaa
     2221 tcttgcttgc cctcaaagca ttttaaaaga aggattaaag cgattagtta aatatttaaa
     2281 agatcaagaa aaataagatc aacaaattga aggtaattta agaagcacta aatattagtg
     2341 cttttattta ataagaaagg agaaataaat gaaagcagta cttttattac atggtttttt
     2401 atcaaacaaa tacgatttta aaaatttaat accgatttta gaaaagtatt atgatcatat
     2461 agaggtaatt acttttccag gtcatagtgt tgatgaaagc tatgataatt tcactttagg
     2521 aaatacgcta gaacttttat tttctactta tg
//
LOCUS       DAOSGS010000120         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_39718_length_2539_cov_2.115137, whole genome shotgun sequence.
ACCESSION   DAOSGS010000120 DAOSGS010000000
VERSION     DAOSGS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39718_length_2539_cov_2.115137"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2462
                     /locus_tag="PLQ22_04415"
     CDS             <1..2462
                     /locus_tag="PLQ22_04415"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH59238.1"
                     /translation="IKPILDSMPKDKLKFVVWLVPSNSILEQTIKNLTNKNHPYRQKI
                     DLDFNNKVEVYTKEELINAQNFNPTVVSEQLSICVLSYDSLRSNKKEGRKVYQENSNL
                     TPFKELYNTPETLIENIDETALMQVINQLSPVVIVDESHNATSELSIEMLNNLNPSFI
                     LELTATPKENSNIITYVEAIKLKKANMVKLPVIVYNRHGKEDVLVDAIDLRNNLEKEA
                     KNNKKYIRPIILFQAQTQGNENHETFEKIKKSLIEIGIPEKEIAIKTADINELKNLDL
                     LSPDCPIKYIITVNALKEGWDCPFAYILATLANRNSPVDVEQILGRILRQPYAEKAES
                     IFLNMSYVLTSSKDFLNTLENIIKGLNRAGFTDKDYYAESSDDSDEVEATDNQVKIEK
                     TINNKQLNIENLEDLKDIEETEINNADDITFDIESIVNRLDSILLDDSLEGSSINNTI
                     KNNDMNNTLNKMLEIANQRNNEYAEKFSENNDQLDVPSQVGEKMKTYKMVDAFKEDAL
                     HTVMPQFFIKTPPSIFFEESKVLLSKENLSEGFILSDKDIMIDFDNIDDNIYKVDVVE
                     GSETTPKYTRMSINESEYFRKYISKASDSDKITTCTEIIYKNLDKIDYVSDKELRPYI
                     KRIISNMTKDQILGMEASLTSYTKKIKTKIIELRDTYLETRFEQLIESNQIVCNPSYT
                     FQEYIAPANVVSGIVKSLYTSEEKLSPYEHNAILAIASLDNVSWWHKIIEKKDFWLNG
                     FINHYPDFVIKTKKGNVVLVEIKGDYLDGTDSKRKVKLGRMWENKLGNNFHYFMVYDR
                     KELNIEGVKPFDKFMEIIKAL"
BASE COUNT         1081 a          278 c          411 g          769 t
ORIGIN      
        1 caattaagcc tatattagac tcaatgccta aagataaatt gaaatttgta gtatggttag
       61 taccttcaaa ttcaattttg gaacaaacta ttaaaaattt aactaataaa aatcatccgt
      121 acagacaaaa aattgattta gatttcaata ataaagttga agtatataca aaagaagaat
      181 taattaatgc acaaaacttt aatccaacag ttgtttccga acaattaagt atatgcgtat
      241 taagttatga ttctttgagg agcaacaaaa aagaaggaag aaaagtttac caggaaaatt
      301 ctaaccttac accatttaag gaactatata atactcctga aacattaata gaaaatattg
      361 atgaaacagc tttaatgcaa gtaattaatc agctttctcc agtagtcatt gttgatgaaa
      421 gtcataatgc cacaagtgag ttaagtattg aaatgcttaa taatttaaat ccttccttta
      481 ttttagagtt aactgcaaca ccaaaagaaa atagcaatat tattacatat gttgaagcta
      541 taaaattaaa aaaagctaat atggtaaaat tgccggtaat agtttataac aggcatggaa
      601 aagaggatgt attagtagat gcaattgatt taagaaacaa tttagaaaaa gaagcaaaaa
      661 ataataagaa atatataaga ccaattattt tgtttcaagc ccaaacacaa ggtaatgaaa
      721 accatgaaac atttgaaaaa ataaagaaaa gtctaataga aattggtatt cctgagaagg
      781 aaatagctat aaagacagct gatataaatg aactgaaaaa tctagatcta ttgagccctg
      841 attgtcctat aaaatatata ataacagtaa atgcattaaa agaaggatgg gattgtccat
      901 ttgcatatat acttgccact cttgctaata gaaattctcc cgtagatgtt gaacaaatat
      961 taggaagaat attaagacaa ccttatgctg aaaaagcaga aagcattttt ttgaatatgt
     1021 catatgtttt aacatcatca aaagattttc taaatacatt agaaaatatc atcaaaggac
     1081 ttaatagagc agggtttact gataaggatt attatgcgga atctagcgat gattctgatg
     1141 aagttgaagc tacagacaat caagtaaaaa ttgagaaaac aataaataat aagcagctaa
     1201 atattgaaaa tctagaagat ttaaaagata ttgaagaaac agaaataaat aatgctgatg
     1261 acataacttt tgatattgaa tctattgtaa atagattaga cagtatattg ttagatgatt
     1321 ctcttgaagg ctcgagtatt aataatacta ttaagaataa tgacatgaat aataccttaa
     1381 ataaaatgct ggaaatagca aatcaaagaa ataatgaata tgcagaaaaa tttagtgaaa
     1441 ataatgatca attagatgta ccatcacaag taggtgagaa aatgaaaaca tataaaatgg
     1501 ttgatgcttt caaagaagat gcattgcata cagttatgcc ccaatttttc ataaagactc
     1561 cccctagcat attttttgaa gaatcaaaag tattattatc aaaagagaat ttatcggaag
     1621 gattcatact tagtgataag gacataatga ttgattttga taacatcgat gataatatct
     1681 ataaagttga tgttgtagaa ggaagtgaaa ctactcctaa gtatactagg atgagtataa
     1741 atgagagcga gtactttaga aagtatattt cgaaagcttc agatagtgat aaaataacga
     1801 cttgtacaga aataatatat aaaaatttag ataaaattga ttatgttagt gataaggaac
     1861 taagacctta tattaaaaga attatatcta atatgacaaa agaccagatt ttaggaatgg
     1921 aagcgtcatt gacttcgtat acaaaaaaaa ttaaaaccaa aataatagaa ttaagagata
     1981 cctatttaga aacaagattt gaacaattaa ttgaaagcaa tcaaatagta tgtaatccta
     2041 gctatacttt tcaggaatat attgcaccag ctaatgtggt ttcaggaata gttaagtcac
     2101 tatatacttc tgaagagaaa ctaagtcctt atgaacataa tgcaatatta gcaatagcat
     2161 cgcttgataa tgttagttgg tggcataaaa ttattgaaaa aaaagatttt tggttaaatg
     2221 gatttattaa tcattatcct gattttgtta ttaaaacaaa aaaaggtaat gtggtattag
     2281 ttgaaataaa gggcgattat ttagatggta ctgacagcaa aagaaaggtt aaattaggca
     2341 ggatgtggga gaataaatta ggtaataatt ttcattattt tatggtttat gataggaagg
     2401 aattaaatat tgaaggggtt aaaccatttg ataaatttat ggaaataatt aaagcactgt
     2461 aaattaaacg ataaatctaa tacaatatta tttctttaag ttaatattca aaggggagaa
     2521 tgatatgagt aatgaaact
//
LOCUS       DAOSGS010000121         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_42076_length_2441_cov_3.883487, whole genome shotgun sequence.
ACCESSION   DAOSGS010000121 DAOSGS010000000
VERSION     DAOSGS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42076_length_2441_cov_3.883487"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1563)
                     /locus_tag="PLQ22_04420"
     CDS             complement(469..1563)
                     /locus_tag="PLQ22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59239.1"
                     /translation="MQNTLWRFSDDWYSRKIYYDIYDMIVIVLFFSMLFQKNNPVFWN
                     HLSRLGLLFIVISGITTIIATHIYPGAVRASYSSLRIDDELYYSSVKHLGIGSYGYMT
                     AIVALFPTLIYFYKKGNIDWLNRRRAIVLTVFLFIVLLYAQIFANIIVALIILLMAYF
                     GEANFKRNIFVIGLIIIISLIIPKYFWTNSLLNISDYFPSDSNIHYKFVELADYTLTG
                     ERFDRANPIGGRAERYPMLFEAFKASPFFGDASYYSKYDTEMGHGGHLYWMSRLALWG
                     VFGFLGYLLILYKIFTPVFNLFDKEFRFYYLLSTLAIIMLGFMKALNTTEIYFILLII
                     IPGLYMGVRNKDLLLKTSKEKLPILTTAKP"
     gene            complement(1812..>2441)
                     /locus_tag="PLQ22_04425"
     CDS             complement(1812..>2441)
                     /locus_tag="PLQ22_04425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH59240.1"
                     /translation="YNTVDYHELEISLSSYNSGNFLYLGGAAKVKGFYTMVKALDFLD
                     ENVAVYFGGSYNVPRKQMSVLKKIAMFILLYGRKRKKAIEKMNSHPKARVIGMTYKVN
                     EYLQNICCLVSPFSTPHFARPVIESYLYKKPAIGTDVKGMEEIIEHKKTGLIVKKENA
                     KELAEAINWMASHPNEAKKMGEYAYHIAKTKFTQNNIQKFEAIYQSLNS"
BASE COUNT          908 a          424 c          313 g          796 t
ORIGIN      
        1 acttttgatc aaaaataagc aaaaccttgc aatatggaac acctaaaatc aatttttgat
       61 caacagtata ttgttgataa acaggactta aatatgtttt aaggatcatc taacttagtt
      121 gggaacccac ctcagagcga attgaattta acctcatttt agcaaatagt tttagtttca
      181 ttcaattata atggaactaa atgtcattgc aatttactag cgaattatga acttaagtca
      241 gttttgtatc ttatcgtaat taaagttctt gtaaaatgtc gtaatttagt agggaaaata
      301 gaaatacaaa gctatcagta tcttattgca ttctttaatt ttttttcaca cgtaaaagcc
      361 ttcttttctt ataatcaaac aaatcaaaga actaaacaca aaaagtaaaa aataacaaac
      421 agttcactca ttaaaaagcc ctcaataaat attataaaat taagaacatt acggtttagc
      481 agttgtcaat atcggcaatt tctctttact agtttttaac agtaaatctt tatttctcac
      541 tcccatatac aaaccgggaa taataatcaa tagtataaaa taaatttcag tagtgttaag
      601 agccttcata aaacccaaca tgattatagc aagagtggat aacaagtagt agaatctaaa
      661 ttctttatca aaaagattaa aaacgggagt aaaaatctta tatagaatta acagatatcc
      721 taaaaaccca aaaacacccc aaagagctaa acgactcatc cagtataaat gaccgccgtg
      781 ccccatttca gtgtcatatt tactgtaata actggcatct ccgaaaaatg gactagcttt
      841 aaatgcttca aaaagcatcg ggtatctttc cgctcttcct ccaataggat ttgctctatc
      901 aaacctttca ccagttaagg tgtaatctgc taattcaaca aatttatagt gtatatttga
      961 atcagaaggg aaataatcgc ttatatttaa aagagaattt gtccagaaat attttgggat
     1021 tattaatgaa atgataatta ttaaacctat tacaaatata tttcgtttaa aattcgcttc
     1081 tccaaaataa gccatgagaa gaattattaa ggctacaata atattagcaa aaatttgtgc
     1141 atataataaa acaataaaaa gaaaaacagt aagtacaatt gccctacgtc tatttaacca
     1201 gtcaatatta cccttcttat aaaaatagat taatgttgga aacaatgcca ctatagctgt
     1261 catataaccg tacgacccaa tacctaagtg ttttacacta gaatagtaca attcatcatc
     1321 aattctcaag gatgaatacg atgctctaac agcacccgga taaatatgag tagcaataat
     1381 cgtcgttatc ccacttatta ctataaataa aagtcctaat cttgataaat gattccagaa
     1441 aactggatta tttttttgaa aaagcataga aaaaaacaaa acaattacaa tcatgtcata
     1501 aatatcataa tatatctttc tactatacca atcatcggaa aatcgccaca gcgtattttg
     1561 caaaatgagt ccatagagta atccaaaaat taatgcgtat ttaacactat ttaaagttag
     1621 cgctttatta tgatatagta ctacaaaaac aataaatatt ggcgcccaca aatatcgaaa
     1681 attccctaat acaataaaat ttttattaaa cacatatagt aaatcactcg ctaataacat
     1741 gacggaaatt ataccgacta ttgtaaggtt acgtaaaaag tacatgatgg tttataatta
     1801 attctttata tttatgaatt aagagattga tagattgctt caaacttttg aatattattc
     1861 tgggtaaact tggtttttgc aatatgataa gcatactctc ccattttttt cgcctcatta
     1921 ggatgagagg ccatccaatt aatagcttca gccagttctt tggcattttc ctttttcacg
     1981 attagtcctg tctttttatg ctctattatt tcttccattc ctttaacatc tgtgcctata
     2041 gccggttttt tatacagata agattcaatt acagggcgag cgaaatgagg tgttgaaaat
     2101 ggagagacca aacaacaaat attttgtaaa tactcattta ctttataggt cattccaata
     2161 acccttgctt ttggatgact attcatcttt tcaatagcct tttttctctt tcttccatat
     2221 aaaagtataa acattgctat tttttttaga acgctcattt gctttcgagg cacattataa
     2281 gaaccgccaa aataaaccgc aacattttca tctaaaaaat caagcgcttt taccatcgta
     2341 tagaacccct taacctttgc tgctccaccc aaataaagaa agttacctga attataactt
     2401 gagaggctaa tttcaagctc atgataatca accgtattat a
//
LOCUS       DAOSGS010000122         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_47264_length_2257_cov_3.948229, whole genome shotgun sequence.
ACCESSION   DAOSGS010000122 DAOSGS010000000
VERSION     DAOSGS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47264_length_2257_cov_3.948229"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..509)
                     /locus_tag="PLQ22_04430"
     CDS             complement(156..509)
                     /locus_tag="PLQ22_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOH59241.1"
                     /translation="MARKGFRFTQYKDLSGKQKKKSRSLLTSYDRMKYYTVSYGTEAE
                     IFDIANFVLSGRAVLANFNDIPSKEANQMLSFLAGVVYATEGEICQISDTLFLFARKE
                     EYEDGSLYQYIQDTK"
     gene            complement(520..1170)
                     /locus_tag="PLQ22_04435"
     CDS             complement(520..1170)
                     /locus_tag="PLQ22_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOH59242.1"
                     /translation="MGFKEKTLELAEILKDYPNCKVVIATKLFTPEQVEELYNLGYRD
                     FGENRVEMFLEKYHHLEHYKDIKWHFFGNLQTRKVREIASHLDCLHSLDSETTAYELD
                     KRLNKELDCYVQVNISEEKNKCGVPYLQVKNFIRRMANYPKIRIIGLMCIGTMTPNED
                     ILEDEFSRMKELQEEIQDLGLAYAPCTELSMGMSNDFKIALKEGATCIRLGRYFLR"
     gene            complement(1278..>2257)
                     /locus_tag="PLQ22_04440"
     CDS             complement(1278..>2257)
                     /locus_tag="PLQ22_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOH59243.1"
                     /translation="LRCVEEEKKIEKEKRKINLTKKQITSLIVAAVFIVIFVISIFAV
                     KIFGNTEFALFLDENIGQVANIFKLIGNNIGAIFMSVTYIVLAVLIVKLINIVLKYSF
                     RRSTKTKTLINLVSSTIKYAALVITLWMILKSFGVDTTTLLASAGVIVLIVGLGAQSL
                     IEDVIAGLSIVFENEYEVEDIVIIDNFRGEIIEIGLRTTKILDLSGNIKIVNNSDVRN
                     VINMSRALAYVIVEFNYEYDTDIARLRKTFHEEKEELAKLIPEVQGELQFNGVTDING
                     YSVRLRYSMYSKEVDKFPAERSLKGAVKIFSDKHGFKFAKEKIIIESNK"
BASE COUNT          786 a          428 c          224 g          819 t
ORIGIN      
        1 aaatttccct aaataaatac tacttaaact atggacaact ttgccaactt tagtaagcct
       61 agcatttggt atccatgaca taataacatc tgcgatcatt accatttcat ataaattcat
      121 aatggtaatc ataatccata aaacgataat taacattatt tagtatcctg aatatattga
      181 tataaagatc catcttcata ttcttctttt cttgcaaaca aaaacaaagt atcagaaatc
      241 tgacaaatct caccttctgt tgcataaaca acaccagcta aaaaactaag catttgatta
      301 gcttcttttg aaggaatatc attaaagtta gctagtactg ctcttcctga taaaacaaag
      361 tttgcaatat caaaaatctc agcttcagta ccatatgaaa ctgtatagta tttcatccta
      421 tcatatgaag taagtagcga tcttgatttt ttcttttgct taccacttaa atctttatat
      481 tgtgtgaatc taaatccttt tcttgccata ataacctctt tatcttaaaa aatatcttcc
      541 cagtctaata caagtagctc cttcttttaa agcaattttg aaatcattgc tcattcccat
      601 tgaaagctca gtgcaaggag catacgctaa ccctaagtct tgaatttctt cttgaagttc
      661 tttcatccta ctaaattcat cttctaaaat atcttcattt ggtgtcattg ttccaataca
      721 cattagacca ataattctaa tcttaggata gttagccatt ctacgaataa agtttttaac
      781 ttgtaagtaa ggtactccac atttgttttt ctcttcactt atattaactt gaacataaca
      841 atctagttct ttatttaatc ttttatctaa ttcataagca gtagtttcac tatctaaact
      901 atgcaaacaa tctaagtgac ttgcgatttc tcttactttt cttgtttgta agttcccaaa
      961 aaaatgccat ttaatatctt tataatgttc taaatggtga tatttttcta aaaacatttc
     1021 tactctattt tccccaaaat cacgataacc taagttatat aattcttcaa cttgttctgg
     1081 agtaaatagt tttgtagcta taacaacttt acaatttgga tagtctttta agatttcagc
     1141 tagttctaaa gttttttctt taaatcccat acttccaccc tcctaatatt cgcttatata
     1201 tagtataccc tttttctcat aaaatatcaa cccttaaaaa aagaaaaaaa cgagatataa
     1261 atctcgtttt ttactaatta tttattagat tcaataataa ttttttcttt cgcaaactta
     1321 aatccgtgtt tatcagagaa gatcttcaca gctcctttta aactacgttc agcaggaaac
     1381 ttatctactt cttttgaata cattgaatat ctcaatctaa cactataacc attaatatca
     1441 gttacaccat taaattgcaa ttcaccttga acttcaggaa ttaatttcgc taattcttct
     1501 ttttcctcat ggaatgtttt tcttaagcga gcaatatctg tatcatattc gtaattaaac
     1561 tctacaatta cataagctaa agctctactc atgttgataa catttctaac atcagagtta
     1621 tttacaatct tgatattacc agataagtca agaatctttg tagttcttaa accaatctca
     1681 ataatctcac ctctaaagtt gtcaataatt acaatatctt caacttcata ctcgttctca
     1741 aacacaattg ataaacctgc aataacgtct tcaattaaac tttgcgcacc taaaccaacg
     1801 attaaaacga taactccggc acttgcaagt agtgttgtag tatcaacacc aaatgatttt
     1861 aaaatcatcc ataaagtaat aactaaagct gcatatttaa ttgtactact aacaagatta
     1921 atcaaagtct tagttttagt tgatctccta aaactatact tcaaaacaat attaattaac
     1981 tttacaatta aaacagcaag aacaatataa gtaactgaca tgaaaatcgc accaatattg
     2041 tttccaataa gcttaaaaat attagctact tgaccaatat tttcatctaa aaacagggca
     2101 aactctgtat taccaaaaat cttaactgca aagattgaga taacaaagat aactataaaa
     2161 acagcagcta caattagcga agtaatctgt ttcttagtta agttaatttt tcttttttct
     2221 ttttcaattt ttttctcctc ttctacacac cgtaata
//
LOCUS       DAOSGS010000123         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_48470_length_2219_cov_2.682994, whole genome shotgun sequence.
ACCESSION   DAOSGS010000123 DAOSGS010000000
VERSION     DAOSGS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48470_length_2219_cov_2.682994"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLQ22_04445"
     CDS             <1..372
                     /locus_tag="PLQ22_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59244.1"
                     /translation="PDNSTFTERHDLSSKAIKKRYKSLFDRLLAYENMSYKITDNSVI
                     FYIEENIYAQLDIRKGRVDLYLALDPEKYPKLYKLKEANEKGFEATPCLLSVSSKFKE
                     LKAKALIKKLANNYNLISRKE"
     gene            377..793
                     /locus_tag="PLQ22_04450"
     CDS             377..793
                     /locus_tag="PLQ22_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOH59245.1"
                     /translation="MKKQKGATLVEVLVAVTVFAIISIAMFTSFLVMKKMVARQKDYL
                     EFEIICRDIDYYSDKHLKDWDLEYYGAKARKDDNGDGVYEVYYDFDYCLSETEQAYCL
                     EYYYTDQNELIISVYHNETKRIIIDKLNYGGKRYGP"
     gene            783..1007
                     /locus_tag="PLQ22_04455"
     CDS             783..1007
                     /locus_tag="PLQ22_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59246.1"
                     /translation="MAHKNKKDGYVLAMVLILTLLMSVTILTTFTIVYRYVRLSKIHI
                     EDLRNDVYDPDPNNLSNIIITIDEVEYDYT"
     gene            994..1794
                     /locus_tag="PLQ22_04460"
     CDS             994..1794
                     /locus_tag="PLQ22_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOH59247.1"
                     /translation="MTTLKIIFLVFTFILGSVFASFAGVIAYRVPKGKSILKPGSYCP
                     ACNNHLKWYDNIPILSWIILGGKCRNCKAKIGVFSLLMEILGGLGFASAYFMYGDDLK
                     KLPIMIVLMLLVFLFMIIANIDYETHYIYSITLVIFAVFALFITLYKVFMFEVNIWPY
                     IIGAAFGFGFFLLIKVLAKLAMKKDALGSGDVYLVGIAGFLLGIFPLLLAILIATLLG
                     SIIEVTKIKLKKQSRESEIAFGPYLLLGIGILAIYGDAIMDLYWKVMS"
     gene            1794..>2219
                     /locus_tag="PLQ22_04465"
     CDS             1794..>2219
                     /locus_tag="PLQ22_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOH59248.1"
                     /translation="MPLFKCTVLNTLGEKEVIYHEAADDVVLKAELKLNKMHVLKTKV
                     VKEKKENEFLALSSKVKADEFTNFLRQFAVMVNAGIPISDCMNSLRSQKFSKPLRVVL
                     QKIYYDIESGILLSEAFEKHPKVFPSFFVNMVVIGEVSGS"
BASE COUNT          762 a          232 c          399 g          826 t
ORIGIN      
        1 cctgataata gtacttttac tgaacgacat gatctttcaa gtaaagctat caaaaaaaga
       61 tataagtcat tatttgatag attgttagct tatgaaaata tgtcatataa gattactgat
      121 aactctgtta ttttctatat tgaagaaaac atttatgctc aacttgatat cagaaaagga
      181 agagttgatt tatatcttgc tttagatcct gaaaaatatc cgaagcttta taaacttaaa
      241 gaagctaatg aaaaaggatt tgaggcaaca ccttgtttat tgagtgtttc ttctaaattt
      301 aaggaattaa aagcgaaagc tttaatcaaa aaattagcta acaattataa tttaatatca
      361 aggaaagagt agtaccatga aaaaacaaaa aggagctact ttagtagaag tgcttgttgc
      421 tgttacagtg tttgctatta tttctatagc tatgtttact tcatttttag ttatgaaaaa
      481 aatggtagca agacaaaaag attatttaga atttgaaatt atttgtagag acatagatta
      541 ttatagtgat aaacacttga aagattggga cttagagtat tatggtgcca aagctagaaa
      601 agatgataat ggcgatggag tctatgaagt atattatgat tttgactatt gtctttctga
      661 aactgaacaa gcgtattgtt tagaatatta ttacactgat caaaatgaat tgattatcag
      721 tgtttatcat aatgaaacaa agagaattat tatcgataaa ttgaattatg gaggtaaaag
      781 atatggccca taaaaataaa aaagatggtt atgtattagc tatggtttta atattaacat
      841 tattaatgag tgttactatt cttactactt tcacaattgt ttatagatat gtgagattaa
      901 gtaaaataca tatcgaagat ttacgtaatg acgtttatga cccagatcca aacaatttaa
      961 gtaatatcat tattactatt gatgaggtgg agtatgacta cacttaagat tattttttta
     1021 gtatttactt ttattcttgg ttctgttttc gcaagttttg ctggtgttat tgcttataga
     1081 gtacctaaag gtaaatctat tttaaaacca ggttcatatt gcccagcttg taataatcat
     1141 ctaaaatggt atgataatat tcccatttta tcttggatta ttttaggggg aaagtgtaga
     1201 aattgtaaag caaaaatagg tgtgttttct ttattgatgg aaatattagg aggattagga
     1261 tttgcatctg catattttat gtatggagat gatttaaaga aactaccaat aatgatagtt
     1321 ttaatgttgt tagttttcct ttttatgata attgcaaata ttgattatga aacacattat
     1381 atttatagta ttactttagt tatatttgcg gtgtttgctt tatttattac tttatataaa
     1441 gtatttatgt ttgaggtaaa tatttggccg tatataatcg gagcagcttt tggctttggc
     1501 ttctttcttt taattaaggt tctagctaag ttagctatga aaaaagatgc attaggtagt
     1561 ggtgatgttt atttagttgg tatagctggt ttcttgttag ggattttccc gttgcttttg
     1621 gctattttaa ttgctacctt attaggaagt attatcgaag ttacaaaaat taaactaaaa
     1681 aaacaaagtc gtgaaagcga aattgctttt ggtccttatt tattattagg tattggtatt
     1741 ctcgctatat atggagatgc gataatggat ctatattgga aggtgatgtc ttaatgcctt
     1801 tgtttaaatg tacagtttta aataccttgg gagaaaagga agtaatttat catgaagctg
     1861 ctgatgatgt agttttaaaa gctgaattaa agctaaataa aatgcacgtt cttaaaacaa
     1921 aagttgtgaa agagaaaaaa gaaaatgaat ttttggcttt aagtagtaaa gtaaaagctg
     1981 atgaatttac taattttctt cgtcagtttg cggtaatggt taatgctggt attccaatta
     2041 gcgattgcat gaatagtttg cgttcgcaaa agttttctaa accacttcgt gttgttttac
     2101 aaaaaatata ttatgatatt gaatcaggaa tattattatc cgaagctttt gaaaagcacc
     2161 ctaaggtttt tcctagcttt tttgtaaaca tggtagtaat aggagaagtt agtggatca
//
LOCUS       DAOSGS010000124         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_49615_length_2184_cov_4.064819, whole genome shotgun sequence.
ACCESSION   DAOSGS010000124 DAOSGS010000000
VERSION     DAOSGS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49615_length_2184_cov_4.064819"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLQ22_04470"
     CDS             <1..456
                     /locus_tag="PLQ22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018112.2"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HOH59249.1"
                     /translation="TADANSMGIKASKYICNILKDEPCPVSDRYYDEYYQITKEVDCL
                     LAKVYEVGEGDLAVGVVKAFEYGILDIPFAPSNYNAGKVLPARDNKGAIRILEFGNLG
                     LDEEIKDFHRRKLKIRGQAEGREVSMQMTIDDITAVSRQELIGRPRKDK"
     gene            456..1700
                     /locus_tag="PLQ22_04475"
     CDS             456..1700
                     /locus_tag="PLQ22_04475"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HOH59250.1"
                     /translation="MKVKDVLFTKSYTGFYFDDQKAIKAGAKNDGFNYLGEPKTPGFT
                     KIRQAGEAVSIQIILEDGSVGYGDCAAVQYSGAGGRDPLFLADDAIKFLEKEIKPLLL
                     KEDFSEFKSLAEKYDHLVINGKRLHTAIRYGVTQALLSAVAACHKVTMAEIVRKEYKI
                     KGKKYKPVPVFTQSGDDRYVNAEKMIIKEAEILPHALINNVETKLGKKGEILKEYLVW
                     LKNRIKSLGKPGYNPIIQIDVYGTIGLAFKNNVKKMTEYLIDLEQTIAPLKLRIEGPV
                     DADCREETMALLRDIRAALEAKGSQVEIVADEWCNTLEDIKYFADNKAGHILQIKTPD
                     LGGINNTIEAILYCNEKGIGSYCGGTCNETDISAKTTTQIALACSASQILAKPGMGVD
                     EAIMLARNEIARVCAIANWRLK"
     gene            1697..1942
                     /gene="citD"
                     /locus_tag="PLQ22_04480"
     CDS             1697..1942
                     /gene="citD"
                     /locus_tag="PLQ22_04480"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOH59251.1"
                     /translation="MKGQAGTLESNDCLITVEKSSKLEIIINSPVAYLYEDEIKKTIL
                     EVLKEEKQENLKIVMEDKGALDYTIRARLIAAIRRMK"
     gene            1942..>2184
                     /locus_tag="PLQ22_04485"
     CDS             1942..>2184
                     /locus_tag="PLQ22_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOH59252.1"
                     /translation="MRRSLLFVPGDNPGMLQSAGVYNADSIIIDLEDSISLPNKDSAR
                     ILVNEFLKDHKLKKEVIVRINSFDSPYYKEDLELLVS"
BASE COUNT          817 a          249 c          454 g          664 t
ORIGIN      
        1 acagctgatg ctaacagtat gggtattaaa gcatcaaagt atatttgcaa tatcttaaaa
       61 gatgaaccat gccctgtaag tgataggtat tatgatgaat attaccaaat tactaaagaa
      121 gtagactgtt tacttgctaa ggtttatgaa gttggtgaag gtgacttagc cgttggtgta
      181 gtaaaggcat ttgaatatgg aattttagat attccttttg cgcctagtaa ttataatgca
      241 ggtaaggtat taccagcaag agataataaa ggtgcgatta gaattttaga atttggtaat
      301 ttaggtttag atgaagaaat aaaagatttt catagaagaa aattaaagat aagaggacaa
      361 gcagaaggta gagaagtgtc aatgcaaatg acaattgatg atattactgc tgtatcacgt
      421 caagaattaa taggtagacc aagaaaggat aaatgatgaa agtaaaagac gttttattta
      481 caaaatctta cacaggattt tattttgatg atcaaaaagc aattaaagct ggagctaaaa
      541 atgatggctt taactattta ggagagccaa agactcctgg atttacaaag atcagacaag
      601 ctggagaagc tgtttccatt caaattattt tagaagatgg tagtgttggt tatggtgatt
      661 gtgctgctgt tcagtatagt ggtgcaggtg gaagagatcc tttgttttta gctgatgatg
      721 cgataaagtt tttagaaaaa gaaattaagc cattactttt aaaagaagac tttagtgagt
      781 ttaagagttt agctgaaaaa tatgatcatt tagtaattaa tggtaaaaga ttacacactg
      841 cgattcgtta tggtgtaact caagctcttt tatcagcagt tgcagcttgt cataaagtaa
      901 caatggctga aattgttcgt aaggaatata aaattaaagg taaaaaatat aaaccagttc
      961 ctgtttttac tcaaagcggt gatgatcgtt atgtaaatgc agagaagatg attattaaag
     1021 aagcagagat tttacctcat gctttaatta ataatgtaga gactaaactt ggcaaaaaag
     1081 gtgaaatctt aaaagagtat ttagtatggc ttaaaaatag aattaaaagt ttaggtaaac
     1141 ctggatataa tccaattatt caaattgatg tttatggaac gattggtctt gcctttaaaa
     1201 acaatgttaa gaaaatgaca gagtatttaa ttgatttaga gcaaactatc gctcctttaa
     1261 aattaagaat tgaaggccca gttgatgctg attgtagaga agagacgatg gctttattaa
     1321 gagatataag agcagcttta gaagctaaag gcagtcaagt agaaatcgta gctgatgaat
     1381 ggtgtaatac tttagaagat attaagtatt tcgccgataa taaagcaggt cacatcttgc
     1441 aaatcaaaac tcctgactta ggtggtatta acaatacgat tgaagcaatt ctttattgca
     1501 atgaaaaagg tattggatca tactgcggtg gaacatgtaa tgaaacagat atttctgcaa
     1561 agacaacaac acaaatcgct ttggcatgtt ctgctagcca aatcctagct aaacctggta
     1621 tgggagtaga tgaagcaata atgcttgcac gtaatgaaat tgctagggta tgtgctattg
     1681 ctaattggag attaaaatga aaggtcaagc tggaacatta gaatctaacg attgtttaat
     1741 tacggttgaa aagagcagta agttagagat cataattaat agtccagttg cttacctata
     1801 cgaagacgaa ataaaaaaga ctattttaga agtattaaaa gaagaaaaac aagagaattt
     1861 aaagatagtg atggaagata aaggagcact agattataca attagagcaa gattaattgc
     1921 tgcgataagg aggatgaagt aatgagaaga agtttacttt ttgttcctgg ggataatcca
     1981 gggatgcttc aatcagctgg ggtttataat gctgattcaa taatcattga cttagaagac
     2041 tcaatttctc ttccaaataa agatagtgct cgtatattag taaatgagtt tttaaaagac
     2101 cataaattaa aaaaggaagt aattgtaagg attaatagtt ttgatagtcc ttattataaa
     2161 gaagatttag aactattagt ttct
//
LOCUS       DAOSGS010000125         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_50178_length_2167_cov_5.429451, whole genome shotgun sequence.
ACCESSION   DAOSGS010000125 DAOSGS010000000
VERSION     DAOSGS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50178_length_2167_cov_5.429451"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLQ22_04490"
     CDS             <1..601
                     /locus_tag="PLQ22_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH59253.1"
                     /translation="KTISESAFAGCYNVRRLTIARVEPEYEYYISESADENPIIIEGA
                     RSSYAGDILTLHSVQVNAEGQTVSAPATWASSNTRVISIDADTGVVTAKETGFATITA
                     VSRSGDLRASTVIMEVKASDLTKVISFEQKSLDRFDSLRELVLDVQDVALLNVPQGFL
                     KLNKQVKIYVPNNAVAFYKAAKIWVDFADYIKPMSELPE"
     gene            727..>2167
                     /locus_tag="PLQ22_04495"
     CDS             727..>2167
                     /locus_tag="PLQ22_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOH59254.1"
                     /translation="MANYGINFDFIILIIVIFELLLGAIRGLYNQAQKTIALLVPIIL
                     NYFLFPLVINILFSLKGYMAFSRVAVTIIGLKGHANLAITIGNYILLYLVFYFLIIYF
                     FKTIRRRNLLFFLGDKKPASRALGSALSLGSSYILIFILGYLLSPIVNVNHKSPVSNV
                     IVTQSNHLFEVSKLNEYQNLNIDDYLEIKETRKQFSFGFIDDDFETYQRLVDLNITFE
                     ETSWNEVLMKDEAKMLLLGEKDLFAFTTKNGKKYVIDQIIKLEKGNPNIEILKENRDF
                     IIKYQAYFPVVLGNDLHNRENIMDLIIASKDDIYANSNRYGKMALEKSIEKYQFYFDH
                     KNKLFFMLGVNSLEEYQNKLIAIENNRELLNQYNEKFLVVFATENSYYFKNAKTIIKQ
                     YQAHEEEFLLLNDEIDFASRLIFASNYEELFVKRTWEKRPLLKAYMNDALLNKKIHGH
                     DIYSQYIFYLYTNTYQDDVVLTEAGIDLFF"
BASE COUNT          801 a          242 c          354 g          770 t
ORIGIN      
        1 taaaacaatt tctgaatcag cttttgcggg ttgttataat gttagaagat taactattgc
       61 tagagtagaa ccagaatatg aatattatat tagcgaaagt gcagatgaaa atcctattat
      121 tattgaaggt gctagatcat cttatgctgg agatatctta actttacatt cagtacaagt
      181 aaatgctgaa ggtcaaactg taagtgcacc agcaacttgg gcttcaagta atacaagagt
      241 aatttcaatt gatgctgata ctggtgttgt tacagctaaa gaaacaggtt ttgcaactat
      301 tactgctgta agccgtagcg gagatttaag agcatcaact gtgattatgg aagttaaagc
      361 aagtgatctt acaaaagtaa ttagctttga acaaaaatcg ttagatagat ttgatagttt
      421 aagagaatta gttttagatg ttcaagatgt agcattatta aatgttcctc aaggattttt
      481 aaagttaaat aaacaagtta agatttatgt tcctaataat gcagttgcct tttataaagc
      541 agcaaaaatt tgggttgact tcgctgatta tataaaacca atgagtgaac tacctgaata
      601 gtaagtaaac atagaagaaa aagtgcctta agaggcactt ttttaaattc aaattaaaca
      661 tatcactaat tgaaaaaaaa ggcgtaattt ggtataatat ggataagcaa ggaatggtta
      721 taaaagatgg caaattatgg aataaatttt gatttcatca ttttaataat tgtaattttt
      781 gaactgcttt taggtgctat tagaggactt tataatcaag ctcaaaaaac gatagcctta
      841 ttagttccaa ttattttgaa ctattttctt tttcctttgg ttattaatat tttatttagc
      901 cttaaaggat atatggcttt ttctagagta gccgtgacta ttataggatt aaaaggtcat
      961 gcgaatttag ctatcacaat tggaaattat atccttcttt atttagtatt ttatttttta
     1021 ataatttact ttttcaaaac cattagaagg agaaatttgt tatttttctt aggtgataaa
     1081 aaacctgcaa gtagagctct tggttctgct ctttcgcttg ggtcctcata tattttgatt
     1141 tttattctag gatatctatt atcaccaatt gtaaatgtga atcacaaatc tcctgtttct
     1201 aatgtaattg ttacacaatc aaatcacttg tttgaagtaa gtaaattaaa cgaatatcaa
     1261 aacttaaata ttgatgatta tttagaaatt aaggaaacta ggaaacaatt ttcttttggt
     1321 tttattgatg atgattttga aacatatcaa aggttagttg atttaaatat aacttttgaa
     1381 gagacatctt ggaatgaagt tttaatgaaa gatgaagcaa agatgctatt attaggcgaa
     1441 aaagatttat ttgcctttac aacaaaaaat ggtaagaaat atgttattga tcaaattatt
     1501 aaattagaaa aaggaaatcc aaatattgaa attttaaaag aaaatcgcga ctttattatt
     1561 aaataccaag cttattttcc agttgtttta ggaaacgatt tacataatag agaaaacata
     1621 atggatttaa ttatagctag caaagatgat atttatgcta attccaatcg ctatggaaag
     1681 atggctttag aaaagagcat tgagaaatat caattctatt ttgatcataa aaacaaattg
     1741 ttctttatgc ttggagttaa tagtttagaa gaatatcaaa acaaattaat tgcgattgaa
     1801 aataatcgtg agcttttgaa tcaatataat gaaaagtttt tggtagtttt cgcaacagaa
     1861 aacagttatt attttaaaaa tgctaagaca ataattaaac aatatcaagc ccatgaagaa
     1921 gagtttttat tattaaatga tgaaattgat tttgcttcaa gattaatctt tgcatctaat
     1981 tatgaagaat tgtttgtgaa gaggacttgg gagaaaagac cattacttaa agcatatatg
     2041 aatgatgctt tattaaataa gaagattcat ggccatgata tttatagtca atatattttc
     2101 tatttatata caaataccta tcaagatgat gtagttttaa ctgaagcagg aattgattta
     2161 ttcttta
//
LOCUS       DAOSGS010000126         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_50218_length_2166_cov_3.425391, whole genome shotgun sequence.
ACCESSION   DAOSGS010000126 DAOSGS010000000
VERSION     DAOSGS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50218_length_2166_cov_3.425391"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ22_04500"
     CDS             complement(<1..255)
                     /locus_tag="PLQ22_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH59255.1"
                     /translation="MSKIIELKNIYKTFDDKEVITDLNLEIDENEFVTLLGPSGCGKT
                     TVLRMIGGFEDPDSGEIILNGETINEIPPYYRPINTVFQRY"
     gene            complement(429..1433)
                     /locus_tag="PLQ22_04505"
     CDS             complement(429..1433)
                     /locus_tag="PLQ22_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH59256.1"
                     /translation="MLAIEIFSVSFFAAMILNGITLLFGCIGETITEKAGHLNLGIPG
                     IMYMGGIGAILGAFIYEANATNFNGFLAILCAVFFSVLLSALGGGIYAVLTVSLRANQ
                     NVTGLALTTFGMGLANFLGGSLGKLAQGGSGVFVSVKRTSDAFTFFIPWLSERTGLFG
                     ELFFNSRYGFLTYIALILAVLVHFFFKKTRVGLNLKAVGENPQASDAAGINVTKYKYV
                     AIMVGSIIAGLGGCYFVMNYLKGVWQHGALGDAGWLAVALVIFSLWRPLRSIWGALLF
                     GALTVAYNYLHASTSQQEILKMLPYLVTIIVLIVTSLNKRREDQPPGSLGLHYFREER
                     "
     gene            complement(1420..>2166)
                     /locus_tag="PLQ22_04510"
     CDS             complement(1420..>2166)
                     /locus_tag="PLQ22_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH59257.1"
                     /translation="GMLWGVFPALFKAKFGTNETLFTLMMNYIAIQLVRYFCIKWEKI
                     PGSGEVGIINNVFGSEYPRVGWISNNFLSGIFGNGNYVVLLLLVSVVALAMYVYMKYT
                     KQGYEIKVVGGSVNTANYAAIDVQKVTIRTVLISSAICGFVGMLIVAGVDHSLSPNLA
                     AGRGFTAITVTWLGNFNMGFMLIISAILTFFSAGAKRLASDISLNASMSDVLIGILIF
                     FILISEFFIRYRVKINLKKKEKEELVNASN"
BASE COUNT          809 a          431 c          259 g          667 t
ORIGIN      
        1 atatctttga aaaaccgtat taataggacg atagtatggt ggaatttcat taatagtttc
       61 gccatttaaa attatctcac cactgtctgg atcttcaaat ccaccaatca ttcttaaaac
      121 tgtcgtttta ccacacccag aagggcctag taaggtgaca aattcatttt catctatttc
      181 tagatttaag tcagtaataa cctctttgtc gtcaaaagtt ttgtaaatgt tttttaattc
      241 aatgattttg ctcatctaag acctccttta agtaaaaaaa atagttatga tgttgaacta
      301 cacacaccaa attaacttaa taaaaaagca gctttcattc gactcataac tttctattgg
      361 atacatttta tcatatttta atataaatat caatataatt attaaaataa aaaagatgat
      421 tttttaaatc atctttcttc tctaaaataa tgcaaaccta atgaaccagg tggttgatct
      481 tcccttcttt tatttagact tgttacaatt aacacaataa ttgtaactaa ataaggtaac
      541 atctttaaga tttcttgttg ggaagttgaa gcatgtaaat aattataagc aactgttaat
      601 gctccaaata gtaatgcccc ccaaatactt cttaaaggcc tccataaact aaagataact
      661 aaagcaacag ctaaccaacc agcatcaccc aaggctccat gttgccatac accttttaga
      721 tagttcatta caaagtaaca accacctaaa ccagcaataa tactaccaac cataatggct
      781 acatatttat acttagttac attaatacct gctgcatctg aagcttgagg attttctcca
      841 actgctttta aatttaagcc tactcttgtt ttcttaaaaa agaaatggac taaaacagct
      901 aaaatcaaag ctatgtaagt tagaaaacca tatctactat taaagaataa ctcaccaaat
      961 aatcctgttc tttctgaaag ccaaggaata aagaaagtaa aagcatcact tgttctcttt
     1021 acagaaacaa atactcctga acctccttga gcaagtttac ctaaagatcc acctaagaaa
     1081 ttggcaagcc ccataccaaa agttgttaaa gcaagtccag ttacgttttg attagctctt
     1141 aatgatactg ttagaactgc gtaaattcct ccacctaaag cagaaagtaa aacagaaaag
     1201 aatactgcgc ataaaattgc taagaaacca ttaaaattag ttgcattagc ttcataaata
     1261 aatgccccta aaatagcacc aataccaccc atatacataa taccaggaat acctagattt
     1321 aagtggcctg ccttttcggt aatagtttca cctatacaac caaatagtaa cgtgattcca
     1381 tttagaatca tcgctgcaaa aaaggatact gaaaaaatct caattgctag cattgaccaa
     1441 ttcctccttt tcctttttct ttaaatttat ttttactctg tatctaataa agaattcact
     1501 tattagaata aagaagatta atatcccaat taaaacatcg ctcattgaag cattaagtga
     1561 aatatctgat gctaatctct tagcacctgc actaaagaaa gttagaattg cagaaataat
     1621 aagcataaag cccatattga aattaccaag ccaagttact gtaatagctg taaatcctct
     1681 gccagcagct aggttaggcg ataagctatg gtcaacgcca gctacaatca acatcccaac
     1741 aaacccacaa atagcacttg aaattaaaac agttctaatt gtaacttttt gtacatcaat
     1801 tgcggcatag ttagctgtat taacactacc accaacaact ttaatttcat aaccttgttt
     1861 tgtatatttc atatacacat acattgctaa ggcaactaca cttacaagta aaagtaaaac
     1921 aacatagttt ccgtttccaa aaatccctga aaggaaatta ttagaaatcc aacctactct
     1981 tggatactca cttccaaaaa cattatttat aattcctact tcaccagaac caggaatttt
     2041 ttcccattta atacaaaaat accttacaag ttgaattgca atatagttca tcattaaagt
     2101 aaataaagtt tcattagttc caaacttagc tttaaataaa gcagggaaaa caccccataa
     2161 cattcc
//
LOCUS       DAOSGS010000127         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_61339_length_1898_cov_3.858383, whole genome shotgun sequence.
ACCESSION   DAOSGS010000127 DAOSGS010000000
VERSION     DAOSGS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61339_length_1898_cov_3.858383"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLQ22_04515"
     CDS             <1..440
                     /locus_tag="PLQ22_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOH59258.1"
                     /translation="YKGKANYDYIKLVKEQVKIPVIANGDIKSIEDAEYVLDYTKCDA
                     IMIGRSSIGNPWLIQNIVNHFEGKELFEPTDIERIQMVKKHYELLKNEMGAKLAILNL
                     RSLFSRYLKGLDVKEYKLRLMKITKEQEMNDLFKEMEVKYENS"
     gene            427..1701
                     /gene="lysA"
                     /locus_tag="PLQ22_04520"
     CDS             427..1701
                     /gene="lysA"
                     /locus_tag="PLQ22_04520"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOH59259.1"
                     /translation="MKTVNTNIQNNELYIGNHSLKELAMKYKTPLMVYDEEGIKSKIK
                     LIKDSFSSKKLKTKIIYASKAFIAPYFANILKEEDLCVDATSVSDFRLLEKVGFPMER
                     VFFQGNNKTEEELKLALNLKINAFIVDSFSELKLIEKLAKAKVDTYFRINPYVKVDSH
                     KHIQTANLDSKFGEGIDDVVWDKIIEFYNNAKHVVLKGLQVHLGSQIKELDPYLEAID
                     KISNFKTKLEQKLKYKIKAFDLGGGFGVKYLESDSEFPLKEGLKKIIKRLEKTDFEIV
                     AIEPGRSIVGREGVTIYQCLKLKQSKYYNYCLVDGGMADNIRPALYDAKYSFDCVNNV
                     ISKSKEKYQVVGKFCESGDILGKDIILSKLKENDYIVTYTTGAYCYAMSSNYNGALKP
                     AVLFVSDKGVKEAIRRETVEDYLAVFNFKGDK"
     gene            1701..>1898
                     /locus_tag="PLQ22_04525"
     CDS             1701..>1898
                     /locus_tag="PLQ22_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59260.1"
                     /translation="MKIFDIHSDLLYDLFRGKGDNRFKDIHVKQYNNSVIKGAVWTLY
                     SPDDFDLKEAVNCALKKIDWNE"
BASE COUNT          766 a          172 c          344 g          616 t
ORIGIN      
        1 tgtataaagg caaagctaat tatgattata ttaagctagt caaagaacaa gtgaaaatac
       61 cagtaattgc taatggcgat attaaatcaa ttgaagatgc tgagtatgtt ttagattaca
      121 ctaaatgtga tgcgattatg ataggcagaa gcagtattgg caatccctgg ttaattcaaa
      181 atattgttaa tcattttgaa ggtaaagagt tatttgaacc aacagatatt gaaagaatac
      241 aaatggtgaa aaaacactat gagttattaa aaaatgaaat gggtgcaaaa cttgctattc
      301 ttaatttaag gtctttattt tctcgttatt taaaagggtt ggatgttaaa gaatataaat
      361 taagattaat gaaaattact aaagaacaag aaatgaatga tttattcaaa gaaatggaag
      421 taaagtatga aaacagttaa tacaaatatt caaaacaatg aattatatat aggtaatcat
      481 tctttaaaag agttagctat gaaatataaa actcctttaa tggtttatga tgaagaaggg
      541 attaaatcga aaatcaaact aattaaagat agttttagct ctaaaaaact aaaaacaaaa
      601 atcatctacg catcaaaagc ttttatagct ccttatttcg caaacatctt aaaagaagaa
      661 gatttatgtg tagatgcaac tagtgtaagt gattttaggt tattggaaaa agtaggtttt
      721 ccaatggaaa gagtcttctt tcaaggaaat aataagactg aagaagaact aaaattagct
      781 ttaaatttaa agattaatgc ttttattgtt gattcatttt cagagttaaa attaattgaa
      841 aaactagcaa aagcaaaagt agatacctac tttagaataa atccttatgt taaggtagat
      901 agccataaac atattcaaac tgccaattta gattctaagt ttggagaagg tatagatgat
      961 gttgtttggg ataaaataat tgagttttat aataatgcta aacatgtagt tttaaaagga
     1021 cttcaagttc acttaggctc acaaattaaa gaattagatc cttatttaga agcaatagat
     1081 aaaatttcta attttaaaac aaaattagag caaaagttaa aatataaaat caaagctttt
     1141 gatttaggtg gaggttttgg ggtaaagtat ttagaatctg atagcgagtt tcctttaaaa
     1201 gaagggctaa aaaagattat taagagatta gaaaaaactg attttgagat agtagcaatt
     1261 gaaccaggaa gaagtattgt aggtagagaa ggtgttacta tttaccaatg tttgaaacta
     1321 aaacaaagta agtactataa ctattgttta gttgatggtg gtatggctga taatattagg
     1381 cctgctttat acgatgctaa atacagcttt gattgtgtta ataatgtaat aagcaagagt
     1441 aaagaaaagt atcaggttgt agggaagttt tgtgagtcag gagatatttt aggaaaagac
     1501 attattttaa gcaaacttaa agaaaatgat tatatagtaa catatacaac tggtgcttat
     1561 tgctatgcaa tgagttctaa ttataatgga gcattaaagc ctgcagtttt atttgtaagt
     1621 gataagggtg ttaaagaagc aattagaaga gaaacagttg aagactattt agcagtattt
     1681 aattttaaag gagataaata atgaaaatat ttgatattca tagtgattta ttatatgact
     1741 tatttagagg aaaaggtgat aatagattta aagatattca tgtaaagcaa tataataatt
     1801 ctgttattaa aggtgcggtt tggactttat attctccaga tgactttgat ttaaaagaag
     1861 cagtaaattg tgctttaaag aaaattgatt ggaatgaa
//
LOCUS       DAOSGS010000128         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_70765_length_1728_cov_2.169157, whole genome shotgun sequence.
ACCESSION   DAOSGS010000128 DAOSGS010000000
VERSION     DAOSGS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70765_length_1728_cov_2.169157"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="PLQ22_04530"
     CDS             <1..1516
                     /locus_tag="PLQ22_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH59261.1"
                     /translation="NDSKKVTLSIFLLYFVPGISVGLVASLAIGAKFKYPKYIFLSTL
                     GNIINLLYVMLFGLTITKDQLLLALILAIVYLVIFVILFIFRKKIINRILRPKRDNEF
                     YRNNFRRMKVLYYMLLIPVVKIFFALRYKFKVKKLNFKKLKAPFVVMFNHPSPLDVAY
                     SYAELGFKNRPASLVASYYYTDKKLAKFFYGLGGISKHLYAPDLGAIKKSLKAVRNGW
                     PIGMAPEGRLSAYGCLETITDATPKLLKKLNLPIVFVNIKGAYLTKPKWAHNFRRGRV
                     DVTYELMYEDFDLNQLSDQELLDVIIQKLDYDDFAWQEENKVYYKGKKFANGLENILY
                     HCPVCKSEFTLEAKDHEITCTKCKVKVHLDNYYQFTSDNPLIPKNIRDWYLLQKDLAS
                     KKVKDPNFKMESNTVFKLPDPKGNGFSSVGEGKVVLDHSGLKYQGTKDGKPFEKFFEL
                     HSYPVILFGAGVDIEFYSDNTIYFFELENLKECAKYSIYWEALYNDYLGGKNNG"
     gene            1509..>1728
                     /locus_tag="PLQ22_04535"
     CDS             1509..>1728
                     /locus_tag="PLQ22_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59262.1"
                     /translation="MDEKQLPEKEMTEKQFLQINKRSSFTALFILVAILFASYFLTLL
                     IPQGEYVTVGGIREYQLIEPRQSYSFGKF"
BASE COUNT          622 a          241 c          271 g          594 t
ORIGIN      
        1 aaacgactca aaaaaagtaa ctttatcgat cttcttgctt tattttgtac caggaatttc
       61 tgtaggttta gttgcaagtc tagcaattgg agctaaattt aaatatccaa aatatatctt
      121 tttatctact ttaggcaata tcattaactt actttatgta atgctatttg gacttacaat
      181 tactaaagat caactattac tagctttaat cctagctatc gtttatttag taatctttgt
      241 gattctattt atctttagaa agaaaatcat caatcgcatc ttaagaccaa aaagagataa
      301 tgagttctat cgtaacaact ttagaagaat gaaagtatta tattatatgc ttttaattcc
      361 tgttgttaag atattctttg ccttaaggta taaatttaaa gttaagaagt taaatttcaa
      421 aaaactaaaa gctccgtttg tggtaatgtt taatcaccca tcgcctttag atgtagctta
      481 ttcttacgcc gaacttggtt ttaaaaatag accagcatct ttagttgctt catattacta
      541 cacagataaa aagttagcta agtttttcta tggtttaggg ggaatttcta aacacttata
      601 tgctcctgat cttggagcaa ttaaaaaatc tttaaaagct gtaagaaatg gctggccaat
      661 tggtatggct ccagaaggta gattaagtgc ctatggttgt ttagagacaa tcactgatgc
      721 tacacctaaa ttattaaaga aactaaattt accaattgtt tttgtgaata ttaagggtgc
      781 ataccttact aaaccaaaat gggctcataa ttttagacgt ggaagagtcg atgttactta
      841 tgagttaatg tatgaagact ttgatttaaa tcagttatca gatcaagaat tattagatgt
      901 aattatccaa aaactagact atgatgactt tgcttggcaa gaagaaaaca aagtttacta
      961 taaaggtaaa aagtttgcaa atgggttaga aaacatttta tatcactgcc ctgtttgtaa
     1021 atcagagttc actttagaag ccaaagatca tgaaattact tgcactaagt gtaaagtaaa
     1081 agttcattta gataattact atcaatttac ttcagacaat cctttaattc ctaaaaacat
     1141 tagagattgg tatttacttc aaaaagatct agctagtaaa aaagttaaag atccaaactt
     1201 taaaatggaa tctaatactg tctttaaact accagatcct aaaggtaatg gttttagtag
     1261 tgtaggcgaa ggaaaagttg ttttagacca ttcaggttta aaatatcaag gaacaaaaga
     1321 tggtaaacct tttgaaaaat tcttcgaact tcattcatat cctgtaattt tatttggtgc
     1381 tggagttgat attgagtttt atagtgataa tactatttac ttttttgaac tagaaaactt
     1441 aaaagaatgt gctaaatata gcatttactg ggaagcttta tataatgact atttaggagg
     1501 aaagaacaat ggatgaaaaa caattgcccg aaaaagaaat gactgaaaaa cagtttctcc
     1561 aaataaacaa aagatcatct ttcactgctt tatttatact tgttgcaata ttatttgcat
     1621 cttacttcct aacacttcta attcctcaag gtgaatatgt aacagttgga ggtataagag
     1681 aataccaatt aattgaacct agacaaagct atagttttgg gaaattct
//
LOCUS       DAOSGS010000129         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_73805_length_1680_cov_2.661538, whole genome shotgun sequence.
ACCESSION   DAOSGS010000129 DAOSGS010000000
VERSION     DAOSGS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73805_length_1680_cov_2.661538"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLQ22_04540"
     CDS             <1..245
                     /locus_tag="PLQ22_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59263.1"
                     /translation="FYGLSDNDRTNNRVFKDKQWYYDEGTYGACLFCGKPFLKTGNRQ
                     KYCTENEECQKARKRKNSRDHYKKILINKSLPKKYK"
     gene            413..622
                     /locus_tag="PLQ22_04545"
     CDS             413..622
                     /locus_tag="PLQ22_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013355031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH59264.1"
                     /translation="MAKIDYADGILQLRAKLNLSQEEFAKMLGVSFISVNRWENDKYA
                     PTKLIKVKLIQLFKENNIEVKEVNE"
     gene            622..>1680
                     /locus_tag="PLQ22_04550"
     CDS             622..>1680
                     /locus_tag="PLQ22_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOH59265.1"
                     /translation="MAIRDMQKQTNTNIQEKANLIWEIATHLYGEYKPHEYGKVILPM
                     TVIKRFDDALKDTKKDVVNKSNELNNSSKEVNDKTRHAILKNVAKYDFYNTSKFDFAG
                     LIADPDNIKENFVSYLQSFSDNVKDIIQRFDFFKEIDKMSENDLLYTIINEFNAKKAD
                     FSPNIVTAQDMGYIFEELIRKFSESYDEQAGAHFTARDIIYLMTELLVANDKETLKAN
                     GGSKTAYDMTMGTSQMLTCLDERLKDINEAINLTCFGQEFNPETYAIAKADTLIRGGD
                     ADNMKFGDTLSNDQFEGYTFDYIISNPPFGIDWKKEKDAVEKEAKLGESGRFEAGLPA
                     IGDGQMLFLQNGLKKLKPT"
BASE COUNT          680 a          196 c          284 g          520 t
ORIGIN      
        1 ccttctatgg tcttagtgat aatgatagaa ctaacaatag agtgtttaaa gataagcagt
       61 ggtactatga tgaaggaacg tatggtgcat gtttgttttg tggaaaacca tttttgaaaa
      121 caggaaatag acagaaatat tgtacagaaa atgaagaatg ccaaaaggct agaaaaagaa
      181 aaaactcaag agatcattat aaaaaaattc taattaataa atcacttcca aaaaaatata
      241 aataaaatat aattatttta acttcataaa gaggtctatc atggtaggct tcttttattt
      301 ttgagaaaat ttgaaaaata acttggaatt attcttcata tactagagaa ttttctaaaa
      361 aagtgatata atgttataag aagtgttaac gatttaaagg atggtaaata atatggctaa
      421 aatcgattat gcagatggta ttctacaatt aagagctaaa ttaaatttat ctcaagaaga
      481 attcgctaaa atgttaggcg tttcttttat ttctgtcaac agatgggaga atgataaata
      541 tgcgcctacc aaactaatta aagttaaact tatccaattg tttaaggaaa ataatattga
      601 agtaaaagag gtaaatgaat aatggctatt agagatatgc aaaaacaaac aaatacaaat
      661 attcaagaaa aggctaacct tatttgggaa attgctacac atttatatgg tgaatataaa
      721 ccacatgaat atggaaaagt aattcttcca atgacagtta ttaaaagatt tgatgatgct
      781 ttaaaagata ctaaaaagga tgttgtaaat aaaagcaacg aacttaataa ctcatcaaaa
      841 gaagtaaatg ataaaacaag acatgcgatt ttaaagaatg ttgcaaaata tgatttttat
      901 aatacaagca aatttgattt tgctggttta atagctgatc cagataatat taaagaaaac
      961 tttgtatctt atctacaatc atttagtgat aatgttaagg atattatcca acgttttgat
     1021 ttcttcaaag aaatagataa gatgagtgaa aatgatttac tttatacaat cataaatgaa
     1081 ttcaatgcta agaaagcaga tttttctcca aatatcgtaa ctgctcaaga tatgggttac
     1141 atttttgaag aattaattag aaaattttct gaatcatatg atgaacaagc cggagctcac
     1201 tttacagcaa gagatattat ttatctgatg acagaattac tagttgcaaa tgacaaagaa
     1261 actttaaaag caaatggcgg ttctaaaact gcatatgata tgacaatggg aacatctcaa
     1321 atgcttactt gccttgatga aagattaaaa gatattaatg aagcaattaa tcttacttgc
     1381 tttggacaag aatttaaccc tgaaacttat gctattgcta aagcggatac tttaattcgt
     1441 ggtggtgatg cagataacat gaagtttggt gatactttat caaatgatca atttgaaggg
     1501 tatacatttg actacattat ttctaatcct ccattcggta ttgactggaa gaaagaaaaa
     1561 gatgcagttg aaaaagaagc taagcttggt gaatcaggaa gatttgaagc agggctacca
     1621 gctataggag atggacaaat gttattcttg caaaatggtt taaagaaatt aaaacctaca
//
LOCUS       DAOSGS010000130         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_93473_length_1441_cov_4.521645, whole genome shotgun sequence.
ACCESSION   DAOSGS010000130 DAOSGS010000000
VERSION     DAOSGS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93473_length_1441_cov_4.521645"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLQ22_04555"
     CDS             complement(<1..333)
                     /locus_tag="PLQ22_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59266.1"
                     /translation="MKNKRILIIGIVINIVLLLAFLIGTSLIKKDAPKDKRVKNLSLE
                     IDITNDGTILVTEDWDIEFLEETNEFYRNIYYHKLEEDNPFIANHEAIGNIATFSTIP
                     NSYNQRLLK"
     gene            complement(344..1000)
                     /locus_tag="PLQ22_04560"
     CDS             complement(344..1000)
                     /locus_tag="PLQ22_04560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH59267.1"
                     /translation="MPNLEVLYEDNHLIVVVKEENLLSQKDITEDEDILSLVKAYLKE
                     KHQKLGNVYLGLVHRLDRRVGGVMVFAKTSKAASRLSKAIRERDFEKKYLAICTGKVE
                     SGILKHKLIKKDQMAFESKEGKEAVLEYKVIKYLKEEGLDYTLVDVDLITGRYNQIRA
                     QFALIGHPLINDFKYDYQGKNYFDQLGLWCYELKFPHPITKEQVSFKKIPEGGIWSFK
                     "
     gene            complement(993..>1441)
                     /locus_tag="PLQ22_04565"
     CDS             complement(993..>1441)
                     /locus_tag="PLQ22_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59268.1"
                     /translation="SEDLKKEEILEIDFSPRIIVLGTNFTKYDHYAIKQINKEIDLIK
                     CEVFDSNTLVLEKNYQSQNYLENGFPKSQVFNEIKEHLLMLGDEIVIKEFPHYVAIRR
                     IANFAYLYYDEALVLRVLADGKYKTKAIKNSKDLETALKLLEEAYA"
BASE COUNT          462 a          247 c          148 g          584 t
ORIGIN      
        1 tttcaataat ctttgattat aactattagg aattgtagag aaagtagcaa tattaccaat
       61 tgcctcatga ttagcgataa aaggattatc ttcttctaat ttatgataat aaatattacg
      121 atagaactca ttggtttctt ctaaaaactc tatgtcccag tcttcagtta ctaaaattgt
      181 tccatcattt gttatatcta tttctaagga taaatttttt acccttttat cttttggtgc
      241 atcttttttg atcaaactag ttccaattaa aaaagcaagc aataaaacta tattaattac
      301 aatacctata attaagattc ttttgttttt catctctagc tccttattta aaagaccaaa
      361 tacctccttc aggtattttt ttaaaactca cctgttcttt agtaattgga tggggaaact
      421 ttaactcata acaccaaagt ccaagttggt cgaaatagtt tttaccttga tagtcgtatt
      481 taaaatcatt tattaaagga tgacctatta aggcaaactg agctctaatt tgattatacc
      541 tacctgtaat taaatctaca tcaactaaag tataatctaa tccttcttct ttcaaatact
      601 tgattacttt atattctaaa accgcttctt ttccttcttt actttcaaaa gccatttgat
      661 cttttttgat tagcttatgt tttaagattc ccgattctac ttttccagta caaatcgcta
      721 aatatttctt ttcaaaatct ctttctctaa ttgcttttga cagtcttgat gcagcttttg
      781 atgtcttcgc aaacaccatc actccaccaa ctcttcgatc taaacgatga actagtccta
      841 aatagacatt acctaacttt tggtgttttt cttttagata tgcttttact aatgataaga
      901 tatcttcatc ttctgttata tctttttggc ttaaaagatt ttcttccttg acaacaacaa
      961 tcaaatgatt gtcttcgtat aaaacttcta aattaggcat aagcttcttc taatagtttt
     1021 aaagccgttt ccaaatcttt ggaattttta attgctttag ttttatattt accatcagct
     1081 aaaactctta aaactaatgc ctcatcatag tataaataag caaaattagc aattcttcta
     1141 atcgcaacat aatgaggaaa ctctttaata actatttcat ctcctagcat taataaatgt
     1201 tctttaattt cattaaatac ttgtgattta ggaaaaccat tttctaaata gttttgactt
     1261 tgataatttt tttctaatac taaagtatta gaatcaaaca cttcacattt aattaaatca
     1321 atttctttat tgatttgttt tattgcatag tgatcatatt tagtaaagtt agttcctaaa
     1381 acaataattc tcggactaaa atcaatttct aagatttctt ctttttttaa atcttcgctt
     1441 a
//
LOCUS       DAOSGS010000131         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1747
            NODE_114519_length_1263_cov_6.356788, whole genome shotgun
            sequence.
ACCESSION   DAOSGS010000131 DAOSGS010000000
VERSION     DAOSGS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863276
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 914
            CDSs (total)                      :: 903
            Genes (coding)                    :: 894
            CDSs (with protein)               :: 894
            Genes (RNA)                       :: 11
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114519_length_1263_cov_6.356788"
                     /isolate="AMR_MDS_1747"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLQ22_04570"
     CDS             complement(<1..324)
                     /locus_tag="PLQ22_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH59269.1"
                     /translation="MKNITDRNKNTNTETAKRNVSFIETFFEIFAPTNLPRNSKANSN
                     PQYSEAIFISPSIGPIIHFIGPKVLTNRPADKTISNNSFLLINSLKTFLVKFNGEDSS
                     LVLIFF"
BASE COUNT          416 a          170 c          210 g          467 t
ORIGIN      
        1 aaaaaatatt aaaactaaag atgaatcttc gccattaaat ttcactaaaa atgtttttaa
       61 agagtttata agtaaaaaag aattattact tatcgtttta tctgcaggtc tattagtaag
      121 tactttcgga ccaataaaat ggataatagg accaatgctt ggtgatatga atatcgcttc
      181 agaatactgg ggattgctat ttgctttact atttcttggc aggtttgtag gagcaaaaat
      241 ttcgaaaaag gtttcgatga aacttacatt cctttttgca gtttcagtat ttgtattttt
      301 atttctgtca gtaatattct tcaagtctcc tttcaactat gcattcttgg tcgttttttc
      361 tatagcatat ggagtagtag aaacgaattt tggttattac ttgctgaaaa ttgttaatcc
      421 caattataag tcttcaatta tatcaatacg ctcaatgttt tctcggctgg tgtcctcaat
      481 cttgatcttt ctctttacta taccgatcct ctcagacact tctcaaggtg ggctattggt
      541 gctttctatc tcggcatttt taatggttac gcttttctca atattctatt ttcggcatag
      601 ggtgagtatt gattagtagg ttaacatatt taactacgat tattgaattg tgatatgatt
      661 tgactagttg taaagaatca tataattacg aaataaaaga tatttgtata ttgtagattt
      721 ttagaaaacg aagtttgaga taacttgtgt aattaatacg atgaaaatat atattgggca
      781 ttctcgtgag tataactatt tagaagattt gtacatgcca ctaaagacaa gcttgttatt
      841 taaaaatcat gaaataattt taccacatga caaaagaaat ttaatcagca aggacattat
      901 caaaaagtgt aatctgttta ttgccgaagt atcttttccc tcaacaggat tgggtattga
      961 gctgggatgg gcaaacattt tttaatactc ccattctttg tatttatcga aaaggtacaa
     1021 ctatttctgg atcattaaaa tatttatgtg ataattttat tgagtattcg gatagtgtag
     1081 atatgattga aaaaatctct aatcacataa gttttttaga tgaataagag tttttgtttt
     1141 gcggataatg cagtcaaagt cttcactaaa caattcaaaa aatggtacac taataaacat
     1201 gaaaaactac tttataacat ttgaaggaat agatgggtgt ggcaaagaca cacagttatt
     1261 tag
//
