LOCUS       DAOSHE010000001        25011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_1566_length_25011_cov_4.809064, whole genome shotgun sequence.
ACCESSION   DAOSHE010000001 DAOSHE010000000
VERSION     DAOSHE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25011
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_25011_cov_4.809064"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /gene="infC"
                     /locus_tag="PLD82_00005"
     CDS             <1..392
                     /gene="infC"
                     /locus_tag="PLD82_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG63805.1"
                     /translation="QKIIKIKEIKFRPITDEHDFEYKVKNGVKFLERGDKVKFTVRFR
                     GREMAHEDLGFRVIDKVREIVQQNIAVIEEKKAAFEGRNLIMIMAPVKKTAKRPASGD
                     TDDDEEEDVIEVIEEDTESESDDEDED"
     gene            426..623
                     /gene="rpmI"
                     /locus_tag="PLD82_00010"
     CDS             426..623
                     /gene="rpmI"
                     /locus_tag="PLD82_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG63806.1"
                     /translation="MPKMKTKRGAAKRFKVTAGGKIKRKKAYKNHILTKKSTKRKRDL
                     RHGCYVTDGDYAKVRAMIPYL"
     gene            658..1008
                     /gene="rplT"
                     /locus_tag="PLD82_00015"
     CDS             658..1008
                     /gene="rplT"
                     /locus_tag="PLD82_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG63807.1"
                     /translation="MPRSIPHSARTRRKKHLRQLTKGFFGTASRGFKKARTSMMRALR
                     FAYRDRRARKREMRSLWIVRINAACRICGGSYSRFIGALKKSNIVLDRKVLAHIAATD
                     IDTFKAIYEQAGVR"
     gene            1121..1963
                     /locus_tag="PLD82_00020"
     CDS             1121..1963
                     /locus_tag="PLD82_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63808.1"
                     /translation="MRRCILSMAVLLQVVLVPTLQAARMHHVHTGDMLELNAVALRSG
                     VTASLTSAGEYDLWLQEVVYGISDEINFLLGVPLVSLGGIEDSTILGDIDFQVQARLA
                     QWQADGATMFSTVWLLFGWRAGLGVESGEGKPNPETGLVQTYTPWATGRSSFWLGAGY
                     SFPVSGMACHLNFTWRSETGKSGDPLEFVFRDDAIELGGSVEYTFEITPDFAVRPFYE
                     MEARLAWDETSPVPSRLENTIGFWMRIGQMFRVTGGLNLPVALESPRYLKQEYFFSVS
                     AVFR"
     gene            1990..3012
                     /locus_tag="PLD82_00025"
     CDS             1990..3012
                     /locus_tag="PLD82_00025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG63809.1"
                     /translation="MQHEIDALVARGIEEIQKAGSLRDVETLRVQFFGKKGSLAELQK
                     TLGSLPVEERPAFGARMNDARARLGEVLDTKNADLEEQSLNVKLAAESIDISLPGREQ
                     REGGLHPVSDVSRQIFRIFESMGFTVLDGPELEEEYYNFDALNIPVHHPAREDQDSFY
                     VGEGRLLRTQTSPMQIRTFERFAPPLAAISPGKCFRRDAVDATHLHTFHQVEGFLVDK
                     GVSMADLKGVLTTFAHRMFGSETRIRFRPDFFPFVEPGAEVAISCFACGGKGCRTCKQ
                     TGWIEIGGAGMIHPAVFKSVGYDYQEWSGFAFGMGIERIVMKKYGVPDIRMFYENDMR
                     FLSQFH"
     gene            3028..5400
                     /gene="pheT"
                     /locus_tag="PLD82_00030"
     CDS             3028..5400
                     /gene="pheT"
                     /locus_tag="PLD82_00030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG63810.1"
                     /translation="MKALYRWIEEKAQSGMDAGMLSERLTMLGMEVEECHKRGGVIEG
                     LMVGEVLTCEDHPDSDHLHVCRVLVGGEENTIVCGAENVRAKIKVVVATIGTTLPGDF
                     KIKKAKIRGVESRGMICSKEELGLEEKSKGIWILPDDTKTGPAGPEILGEEDWLMALA
                     VTSNRSDCLSINGLAREAAAAAGKKYCPSRPVLAEKADVSAPAIRIDAPELCGRYSSR
                     LVRGVKIGPSPDWMVSRLEACGMRSINNIVDVTNYVLLEYGHPLHAFDLAKLEGGQII
                     VRRALDGEVLQTLDGQERKLDATMLVIADTLRPVALAGVMGGANSEVSDGTTDILLES
                     AWFDPVSIRRTSKKTGLSTEASYRFERTADYGGCLDALDRAVELIVKTAGGKPGPVTD
                     VQARPVEPVEISLDYSFVEERLGFAPPIGDARKILANLGFIVAGNGNQLSLTVPTWRS
                     DVRLAADIVEEVARHYGYDRMPARLFPIRVNDELFAASDMTKRMLQDALVSMGLTEAL
                     NFNFVLESDLAAMQIDPQSCVTVSNPMSADQKYLRPGLLPNLIANVRTNLGRGVRDIA
                     LFEIGKTFVHGDSAPEERLSLGIILSGKAVSSNWIEPGGREYDFFDIKGMAEAVLARL
                     GMTDLSWERNSGSAWHPGRSAMIKNEGTPIGRIGELHPARCKVLDIHQRVVMMEIDTV
                     GRMHGRGEGGTVVEPSRFPSLLRDLAFVVPEDVQSGALMSTIRKAARHLEMLRLVSVW
                     RGEQIGTGNKSLAFSLEFVCREKTLADDEIESIVQNVIKEAGARHGARLR"
     gene            5415..5969
                     /locus_tag="PLD82_00035"
     CDS             5415..5969
                     /locus_tag="PLD82_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63811.1"
                     /translation="MELLPADVLGNAIERLIGHCQSLDRDRTDLARALSRVQEEFVNL
                     RAEHERLIQAHRELQEHHNALENACAVAVERLERMVSSLGTGIPLTPVQDLMHQSAEV
                     VVQDAEAIQESGAPVREEVTQQSGLAVAERVIKPLNIGNFERIVRQAGGVLPEPVESV
                     IEAEDSTPDPVVIESSGCIRDRLF"
     gene            5990..6307
                     /locus_tag="PLD82_00040"
     CDS             5990..6307
                     /locus_tag="PLD82_00040"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOG63812.1"
                     /translation="MGEIVTMHVFGQEYELRINTESGRERLEIVAELVDRKMKDVAAR
                     YVSLTTKEVAVLAALNLADDYIQLQERLSSVAIDPVVDEDSSVRMLEYAGRINAYLGG
                     QTR"
     gene            6308..6488
                     /gene="ssrS"
                     /locus_tag="PLD82_00045"
     ncRNA           6308..6488
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLD82_00045"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6532..8211
                     /locus_tag="PLD82_00050"
     CDS             6532..8211
                     /locus_tag="PLD82_00050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG63813.1"
                     /translation="MKILERVCALKPMTKALVIAVPVCVVLGVLAFARLFNTADDALA
                     TWIGKDGTEYSITRAEAYMSMGQMKDRMLQDKAYQKNHLDNMLFQRLQAMEAIDNKLN
                     LDPGYVKRTNEHMTNNMLIPELLQTLFPRESLEFSLEARKVRMILVKRDGFNHISEPD
                     PNRFKRMADIQKTITNQARLKQEMERLEKATITKDVKKTDAEMAAHETNIQAKAQSVL
                     AMVKKPGADFADIAKQNSDHPSASEGGQVGYVLPRSRSHSPQVMAAIAKLKVGEISGV
                     INTHEGFVIVKVDEIATVTEVNLGEYYTDEGKKNFAKNEAWFASVWSLIDKTIEDQKG
                     KSVFVYRENLVSKDSNALVFEVKHPKVQMKITRGDLLRKMGNVPPPRRSMFGVLDGMA
                     DPAGYSADEMWNFFEWEMSFPILKLAAWLNGIVESDAFQAKLRDSSYSVLASMLSDKR
                     RESATVTDEEISAQYDRAKASYVKRLPGGATEQLSLDEAKEQIRNELRNKKINDSFHN
                     WKNELFTRYQAKIHENRFEVIKPEPPKQNNPQQANPKNGNKPDDGKKNSGK"
     gene            8232..9530
                     /locus_tag="PLD82_00055"
     CDS             8232..9530
                     /locus_tag="PLD82_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG63814.1"
                     /translation="MQEFLFPAPSATYFTGLADSVEAEVLALKKSGKTDEAVRAENRM
                     NGFLVRAFGADQARSSFCRDAVSRMYRHGRIDLMGEWMQRVVWTRDRDFRFIHAWLSN
                     VNPNIAGEAVTALGKSGDPAISEEFARRLVDSESLPMRLRLIKALGELGLPGTMDSVL
                     SACDSRLQNRNYLPFAEEMAIITTMGRIGEKLENPHRVGRLLADRWDTLGTRKLQSEE
                     LLSLRTNILYALSRAGQEETGRIYRSILDQNAPGPVLDAVIRSAGRTRIEVARDVLPR
                     ALTMELTEDVEHDCIEKAAEFSGLGDGRELLFPTEWLVKRLERNRDRSAILKILAGHP
                     PVFSKKVFLDILKSDRAITLREADVIARHLQEGVVRDSAGLLAGKIRANPEDELVPLW
                     MQVLASLRVPETRMILVDLSRDGLPGIRILAEQILDSWKP"
     gene            9582..10160
                     /locus_tag="PLD82_00060"
     CDS             9582..10160
                     /locus_tag="PLD82_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63815.1"
                     /translation="MNKGHNMRLTLLFFLGGLIVLVACGKDDKFSEYKVVVGNEAETL
                     IVPAQDAISMPFSGGPEDIPVAAERLYIEMRRLGRFFGGPLRLECTVEPDWTAKKLTG
                     RILFPLAPGEGAREYADRPESPVKPEIGRVSGGRYSTILFDGPLQALEAGHLKLRDAV
                     GKASGQCVFMFEGTLKEIDKRYRVRLMARTGE"
     gene            10163..11128
                     /locus_tag="PLD82_00065"
     CDS             10163..11128
                     /locus_tag="PLD82_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63816.1"
                     /translation="MKKHVFAGVLVLMMVPLAGCFSEPEARLEERVQRIEVLLEPSEK
                     TLLDSGKYSLAAESLRKRLSENKELERRYRDVIQEENIGFFDVESVLRFYHGSMRARA
                     RLFRFIAMLADPEVTAFNERRFADLARSLEARFALESGLEDLYRKQTAPVLDPVRDDD
                     IAREYALDSVVKPFAVVYSVMNPGERELLVTDKAGNAAGNLRVRMSADPALQGALTTI
                     RVLLPETRSIEDPGRVFSIAAALAVTNREARQDLVKIADWISAASKTGRLVSKTSIGT
                     AFPDEMTRLVNILPKPDVKTKRLFLLEREISWFSLVVDEKINFYN"
     gene            complement(11164..11478)
                     /locus_tag="PLD82_00070"
     CDS             complement(11164..11478)
                     /locus_tag="PLD82_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOG63817.1"
                     /translation="MSPQQQKNMQEIKIELEGKIGEGAYSNLVIIAHSDSEFILDFAT
                     FLPGNPSARVHSRIIMTPKHAKLLLHSLKQNIGQFEEQFGEIRISPQESFIGGDGRPV
                     AN"
     gene            11551..12030
                     /locus_tag="PLD82_00075"
     CDS             11551..12030
                     /locus_tag="PLD82_00075"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG63818.1"
                     /translation="MEHMSVRAALPRDAGAISALLGGYAERGLLLPRPVEEILESIRD
                     FSVLVDGADEVIGCAALRIYDTALAEIRSLAVREEYKKHGAGRRLITACEDDARRNGI
                     SRVFALTYVPEFFERLEFTRIPKETLPQKIWRDCFQCKHFPNCGEIAVVKTLSSKAE"
     gene            12031..13095
                     /gene="corA"
                     /locus_tag="PLD82_00080"
     CDS             12031..13095
                     /gene="corA"
                     /locus_tag="PLD82_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOG63819.1"
                     /translation="MLAIKKRHKTGLAPGTMVHIGKYEKLPVRVHSFLWTKEQCREEA
                     GIPESETLAGLAERNGILWVNVDGLHDVEKIGAIGSRFSLHPLELEDIVNTEQRPKIE
                     DFDDHISVMLKMLSYDTQANRVESEHVTLVLAKNTILSFQEERPGDVFGPLRDRLRSN
                     RGNIRCQQPDYFLYSLMDAIVDNYFVCLERIGERIDEIEDRFFRNESKDMMGEIHDLK
                     RELLYLRKSVWPLRDIIREVLRMKTALVSESTEAYFRDLLDHAIQIIDNLELFREMLN
                     GLANLYMSSLSMRLNEIMRVLTIISTIFIPLTFITGVYGMNFKTLPGSGWEYGYWAIW
                     GVMLSVALGMLGWFKHRKWF"
     gene            complement(13192..14316)
                     /locus_tag="PLD82_00085"
     CDS             complement(13192..14316)
                     /locus_tag="PLD82_00085"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HOG63820.1"
                     /translation="MEKSIPAWKDKILFTPGPLTTSPTVKQAMLRDLGSRDYAFIGVV
                     RDIRNRLLDLAQTKKGEYECIIMQGSGTFSVEAVISSSIPKDGKLLVLVNGAYGRRIM
                     TIAKVHSIAVDSIDFGEDTLVDPARVEEALKKDPSITHVAIVHCETTTGIINDINAIG
                     RVVTRAGKRYFVDSMSAFGGVELDVPSGNIDFIVSSSNKCVEGVPGFAFIIAKKSALD
                     ECRGRARTLTLDLYAQWEGLEKDGQFRFTPPTHSLLAFHQALLELEAEGGIPARAARY
                     RANHDFMIQGMRDLGFKPFLPDSLQGNIITSFYYPADPKFDFATFYGKLNDRGYVIYP
                     GKVSNAECFRIGHIGRMNTNDGRDLLAAIRDVVKEMGLTL"
     gene            14434..14505
                     /locus_tag="PLD82_00090"
     tRNA            14434..14505
                     /locus_tag="PLD82_00090"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14466..14468,aa:Cys,seq:gca)
     gene            14538..14623
                     /locus_tag="PLD82_00095"
     tRNA            14538..14623
                     /locus_tag="PLD82_00095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14572..14574,aa:Leu,seq:taa)
     gene            complement(14884..14967)
                     /locus_tag="PLD82_00100"
     tRNA            complement(14884..14967)
                     /locus_tag="PLD82_00100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14931..14933),aa:Leu,seq:caa)
     gene            15179..15652
                     /locus_tag="PLD82_00105"
     CDS             15179..15652
                     /locus_tag="PLD82_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOG63821.1"
                     /translation="MKVEYINPFVVATQSVFRAMMNVEAVAGKPFLFDPVTGSHAYDI
                     SGIIGLSGETTGAVVLSFTKLCALKSVSAFSGMEIKIFDQTVVDAIGELINIIAGNAK
                     KDLTDFKLNISLPSVVTGHSHRLNWPTGVPVIMIPFTSELGEFSISVSLREPSKR"
     gene            15764..18193
                     /gene="leuS"
                     /locus_tag="PLD82_00110"
     CDS             15764..18193
                     /gene="leuS"
                     /locus_tag="PLD82_00110"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG63822.1"
                     /translation="MAERDYKFTAIEKKWQTCWDEQKTFKVTEDPSVPEEKRFYCLDM
                     FPYPSGAGLHVGHPEGYTATDIICRYKRMRGYAVLHPMGWDAFGLPAENYAIKTGTHP
                     RTSTNRNIDNFRRQIKALGFSYDWDREISTCNEDYFRWTQWIFLQLFKAGLAYQAVVP
                     INFCPSCRTGLANEEVKDGKCDRCQSLVERKDMKQWMLRITAYADRLLSDLDLLDWPE
                     PIKLMQRNWIGKSTGAEVDFAIEGSSKSLRVFTTRPDTLFGATYMCIAPEHPLVAELT
                     TEAQREAVRNYVSSAKNKSDLERTDLAKDKTGVFSGSHAINPVNGEKIPVWIADYVLI
                     SYGTGAIMAVPAHDERDFAFAKKFGLPIIEVVSVDGKAHGELTEALTVDGIAINSGAF
                     TGMKTPEVKQKITAWLSDEGKGKESVQYKLRDWVFSRQRYWGEPIPVVHCQKCGVVPL
                     PESALPLTLPEVTSYQPTGTGESPLAAMEDWVQTTCPACGGRAWRETDTMPQWAGSCW
                     YYIRFLDPKNTEAFASPEAIKYWLPVDLYVGGAEHAVLHLLYSRFWHKFLYDQGFLPG
                     PEPFTRLVNQGLILGEDGQKMSKSLGNVINPDDVIAEHGADAMRLYEMFMGPLEMSKP
                     WNTNGILGIRRFLERAWRLFEKEIVSGGSSGEIERLRHKTIRKVTEDLDAMAFNTAIS
                     ALMVYVTELGKLESLSRIDGETLALLLAPFAPHLGEELWERLGHGQTIAYEPWPVFDS
                     DKTQDDTIEIVFQINGKVRDKVQVHASVSGPEMESRARSSDKIQPWLEGKEILKVITV
                     PGKLVNIVIKG"
     gene            complement(18305..19279)
                     /gene="rfaD"
                     /locus_tag="PLD82_00115"
     CDS             complement(18305..19279)
                     /gene="rfaD"
                     /locus_tag="PLD82_00115"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOG63823.1"
                     /translation="MIIVTGGAGCIGSATVWALNTRGRQDILIVDRLGKGEKWKNLVS
                     LQYRDFIQPDDFLRGITSGTFTPAGIEAVVHLGACSSTTETDADYLMSNNYAWSVSLA
                     EWCLKHDIRLVYASSAATYGDGGTGYHDDPESARSLKPLNMYGYSKHAFDLHAINRGW
                     LHKMAGLKFFNVFGPNEYHKGDMRSVVLKAWSALNSGSEYPLFRSHREGYADGEQRRD
                     FVWLKDVTKVILFFLDHPDINGIFDVGSGTASSFNQLLTAVFDAMGKPPSITYIDMPL
                     HLRDRYQYYTCADISRLRSVGYDSPCTPLEEAVREYVQEYLAQGKHLS"
     gene            complement(19276..21147)
                     /locus_tag="PLD82_00120"
     CDS             complement(19276..21147)
                     /locus_tag="PLD82_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63824.1"
                     /translation="MLPKLRKSLLVQTAVIALFSLAGFLILFYVVLQAVADARVLKRM
                     FGFDSFAGFLLTSGGGILAVILLAGLLFGLVRRIFFSRTIKGEADALHRILTRFESGD
                     LSQDPDELASLPLVIRNDVSALRESLVARFVNIRQSLNELGEVARNLQQMSVDSSLRI
                     SFLNSQLDTMNTTLSTLQETLEEVQIPATNRLQTVLVAGDGAPASSLITALADCGSCG
                     TIFHTVSSGQTDPQATPLPAAKLPDFTSLLQNLSDSLVFLTSPLALDDDTTNRLIAHA
                     VPVFGYGNRVSTLDHESLLALLARYRIPLLPWMEISSVDQGVQFASTHKGPWRIEAGR
                     LQADLNPVFNEIPALSDFLSAHPGIFNDVIPVKILCGEMAREYSVIALCDRTTLFLLG
                     CAELDIAAEDNDTGLATSGSGAFTPAFSDNDPILRRIRTLILGRLHGALRREGIIWRG
                     FVTLRLHISSDGHPFLQNIFFGLLSPESDAILIATKEYLIPACLAALRDELEVFEPPV
                     KSHNAIAIVFSGEGRLPEAPAGTFIIPSASDSSLKFSLVSSRRTLDETIYSAYLSALP
                     VIRSGTYSCRQDIGGRTLLAHKTRKQKHLRIPGSSRIRSLYELLLGTWSGGGRGMER"
     gene            complement(21191..21898)
                     /locus_tag="PLD82_00125"
     CDS             complement(21191..21898)
                     /locus_tag="PLD82_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63825.1"
                     /translation="MTRISTLVSIFVFAFSGVLAGQAIISDSPEHALISSENHDILAP
                     GSSRDFRHPRITYRHRHPAPVITRIAMHQSGDTIPWNAGREIPLIEDELPGSLYSLSS
                     NANGWTLKIREQPLLSSVVILGPTIVTAQGRITWALRSITPVGNTNKPEILWCGTLLG
                     PANGLYFLATSLPVDDPGMGRVFRIEIPKQVVYGYWETGIRGLNITPGSFLRVRLYGL
                     GHGYQGAQARTYRIPIH"
     gene            22032..22766
                     /locus_tag="PLD82_00130"
     CDS             22032..22766
                     /locus_tag="PLD82_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63826.1"
                     /translation="MEVCFTASVRPLASARGMYRFSFAASLVFALLAVLGLESIGSGF
                     FVFTMVALWFLGVGCWQVRQARRLKSSVILIDDDGLLASISGREQFHPASEVCAGHGS
                     IARSAGFGKQYRICRAVRICQSVVVADIETAVRSRLPEQHVFSGDVLPLDAAEYDRFL
                     ATLKERGWDRPCMERSETPERGRISVLRVVYASGVLLCILGWIFLDNTRLALAFLLGG
                     LGLAGLLVNSLYTRNGPSTPGEERMS"
     gene            22829..23211
                     /gene="rnpB"
                     /locus_tag="PLD82_00135"
     ncRNA           22829..23211
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD82_00135"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23310..23489
                     /locus_tag="PLD82_00140"
     CDS             23310..23489
                     /locus_tag="PLD82_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63827.1"
                     /translation="MKKIFAIALVAVMSLVVVACKKGDAQAPATNVETKVEKVDVKAD
                     TNAKTEVKKDEKAGK"
     gene            complement(23954..24433)
                     /locus_tag="PLD82_00145"
     CDS             complement(23954..24433)
                     /locus_tag="PLD82_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOG63828.1"
                     /translation="MEARTVNPFIIAAVQIVRENTGLSVAKDAVSVQQGKFTPAGTGM
                     SLDISGQIHGKIVYEFSKGVAARLSQRMVEKQLDLSMLEAGDFRKLLHSALLELGNQI
                     TARAITLLEQNGINCTISPPKFYLGQDLQLIHPHLRTIVLALKTEFGPFTINIALYA"
     gene            24618..>25011
                     /locus_tag="PLD82_00150"
     CDS             24618..>25011
                     /locus_tag="PLD82_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63829.1"
                     /translation="MADGTQPGSLEKHREWVARVFASGRIPHGILLAGAPGSGKLTLA
                     LEMARRFLCHAPDPGSRPCGTCIACREMLTLRYPDLILFSSRDVVPEVRALTALASRA
                     VETPAGRDRLIQAVRRLFYRVVNRVNGGY"
BASE COUNT         5597 a         6220 c         7609 g         5585 t
ORIGIN      
        1 ggcagaaaat catcaagatc aaagagatta aattccgtcc gattaccgac gagcatgatt
       61 ttgagtacaa ggtcaaaaac ggcgtcaagt ttctcgaacg tggcgacaag gtcaagttta
      121 cggtgcgttt tcgtggtcgc gagatggcgc acgaggatct tggtttccgt gtgattgaca
      181 aggtccgcga gattgtgcag cagaatatcg ctgtcatcga agaaaagaag gcagcgtttg
      241 aaggtcgaaa tctcatcatg attatggccc cggtgaagaa gaccgcgaag cggcccgcat
      301 ccggcgacac ggacgatgac gaagaagagg acgtcatcga agtgatcgag gaggatacgg
      361 aatcggagtc cgatgatgag gacgaggact gagtttcagt ccaatatacg agtggaggtt
      421 gtaccatgcc caagatgaag acaaaacgcg gggcagccaa gcgtttcaag gtcactgccg
      481 gtggcaagat caagcgcaag aaggcttaca aaaatcacat tcttacgaag aagtcgacaa
      541 agcgcaagcg tgatttgcgc catggatgct acgtgacgga cggggattat gccaaagtcc
      601 gcgccatgat cccctatctc tgatcgactg taacgaaaac gcacaaggag cagattcatg
      661 cctcgttcca tacctcattc cgcacgtacc cgcagaaaaa agcatctgcg gcaactgaca
      721 aagggtttct tcgggaccgc cagccgcggg ttcaaaaagg cccgcacatc gatgatgcgc
      781 gctctcaggt tcgcctaccg cgaccgccgg gcacgcaagc gcgagatgcg ctctctctgg
      841 attgtccgca tcaatgccgc ctgcaggatc tgcggcggga gctatagccg ctttatcggc
      901 gcgctcaaaa aaagcaatat cgtcctcgac cgcaaggtct tggcacacat tgcggccacc
      961 gatattgaca cgttcaaagc catctacgag caggccggcg tccgctgagc cggacgtctg
     1021 ccgggaacaa cgagaaagga gcggcaggaa tgccgctcct ttctcgttca cacccatgac
     1081 aggcatgagg ttgttcacca gaaaaaggag gcagcaggaa atgcgcaggt gtattctctc
     1141 catggccgtg ctccttcagg ttgtgctggt cccgaccctg caggctgcgc ggatgcatca
     1201 cgtccatacc ggtgacatgc tggagttgaa cgccgttgcc ctgcggagtg gagtgacggc
     1261 cagcctgacc tccgccgggg aatatgatct ctggctgcag gaggtcgtct atggaatatc
     1321 ggatgaaata aattttttgc tcggcgtgcc gctggtctcg cttggcggga tcgaggattc
     1381 cacgattctg ggcgacatcg attttcaggt ccaggccagg ttggcacagt ggcaggccga
     1441 tggtgccacg atgttctcga cggtctggct cctgttcgga tggcgggccg gattgggggt
     1501 cgagagcggt gagggcaagc ccaatcccga gacgggtctc gtccagacct acacaccgtg
     1561 ggcgaccggg cggagttcat tctggctcgg cgctggatac tcctttcccg tttcaggtat
     1621 ggcgtgccat ctcaatttca cctggcggag tgagaccgga aaaagcggtg atcccctgga
     1681 gtttgtcttt cgcgacgatg ccatcgagct tggcgggtca gtcgaatata ccttcgagat
     1741 caccccggat ttcgccgttc ggccgtttta tgagatggaa gcgcgtctgg cctgggatga
     1801 gacttctccc gtgccgtcgc gtctggagaa cacgatcggc ttctggatga ggatcggtca
     1861 gatgttcagg gtgacgggcg gactcaatct tccggttgca ctggagtcgc ccaggtatct
     1921 caaacaagag tattttttca gtgtctcggc agtctttcgt tagccttgga gctggttcag
     1981 gaggaaaaga tgcagcatga aatagatgcc cttgtcgccc ggggaattga agagatccaa
     2041 aaagcaggca gtctgcgtga cgtagaaacc ctgcgggtcc agtttttcgg gaaaaaaggg
     2101 agccttgccg aattgcagaa gacactgggt tcgctcccgg ttgaggaacg tccggccttt
     2161 ggcgcacgga tgaatgatgc cagagcccgc ttgggtgagg tactcgatac gaagaatgcc
     2221 gatcttgagg aacagtctct caacgtgaaa ctcgcggcgg agtcgattga catttcgctt
     2281 cccggtcgtg agcagcgtga aggcgggctc caccccgttt cggatgtttc acgccagatt
     2341 ttcaggattt tcgagagcat gggatttacc gttctggatg gcccggaact cgaagaagaa
     2401 tattacaatt ttgatgcatt gaacattccg gtccatcacc ctgcccgtga ggaccaggat
     2461 tccttttatg tcggcgaggg gcgactcctc aggacacaga cctccccgat gcagatccgg
     2521 acgtttgaac gctttgctcc gccgttggcg gcgatctccc cgggaaagtg tttccggcgg
     2581 gatgccgtgg atgcgaccca tctccacacc ttccatcagg ttgaagggtt tctcgtcgac
     2641 aagggtgtct cgatggcgga tctcaagggt gtgctgacca ccttcgccca caggatgttc
     2701 ggatccgaaa cccggatccg gttcagaccg gatttcttcc cctttgtgga accgggggcc
     2761 gaggttgcca tcagttgttt tgcctgcggc ggcaagggat gccggacctg caagcagacg
     2821 ggctggatcg agattggtgg tgcgggcatg atccatccgg ccgtgttcaa gtcagttgga
     2881 tatgactatc aggaatggtc aggatttgcc ttcgggatgg gtatcgaacg tattgtcatg
     2941 aaaaagtacg gtgtacccga cattcgcatg ttttacgaaa acgacatgcg gtttctttca
     3001 cagtttcatt gaaaaagggg tactcggatg aaagcattgt atcgatggat cgaagaaaaa
     3061 gcacagagcg ggatggatgc cgggatgctc tcggagcgtc tgaccatgct cgggatggaa
     3121 gtggaggagt gccacaagcg tggaggcgta atcgaaggtc tgatggtcgg cgaggtcctg
     3181 acctgtgagg atcaccccga ttcggaccat ctccatgtct gccgtgtcct ggttgggggc
     3241 gaggaaaaca cgatagtctg tggtgccgag aatgtccggg ccaagatcaa ggtggttgtc
     3301 gccacgatcg gaacaaccct tccaggggat ttcaagatca aaaaggccaa gatccggggt
     3361 gtcgaatccc ggggaatgat ctgttccaaa gaggaactcg gccttgaaga gaaaagcaaa
     3421 ggtatctgga tccttccgga tgataccaag accggtcccg ccggtcccga gatccttggt
     3481 gaagaggact ggttgatggc gctggctgtg acatcgaacc ggtcggactg tctttccatc
     3541 aatggtctgg cacgtgaggc tgccgctgca gcgggcaaga agtattgtcc gtcccgcccg
     3601 gttcttgccg agaaagccga tgtttccgca cccgcgatcc ggattgatgc tccggaactt
     3661 tgcggccggt attcctcccg gctggttcgc ggtgtgaaga tcggacccag cccggactgg
     3721 atggtgagcc ggctcgaggc atgcgggatg cggagcatca acaatattgt cgatgtgacc
     3781 aattacgtcc tgttggaata cggacacccc ctgcatgcct ttgacctggc aaaacttgaa
     3841 ggtggacaga tcatcgtccg gcgcgcgctc gatggcgagg ttctccaaac tctggacgga
     3901 caggagcgga aactggatgc gaccatgttg gtgattgcag acacgcttcg gccagtggcg
     3961 ctggcgggtg tgatgggtgg tgccaacagt gaggtgtcgg atgggaccac cgatattctc
     4021 ctggaaagcg cctggtttga tccggtctca atccggcgga cttcgaaaaa gaccggtctt
     4081 tcgacggaag cttcgtatcg gttcgaacgt acggcggact atggtggctg tcttgatgcc
     4141 cttgacaggg ccgtcgagct gatagtgaag actgcgggcg ggaagccggg tcctgtgacg
     4201 gatgtccagg ccaggcctgt cgagccggta gagatcagcc ttgactacag ctttgtcgag
     4261 gagcggctcg ggtttgcacc gccgatcggg gatgcccgca agattctcgc caacctcggg
     4321 ttcattgttg cggggaatgg caaccagctt tccttgactg ttccgacctg gcgttcggat
     4381 gtccgtcttg cggcggatat cgtggaggaa gtcgcccgtc actatgggta cgaccggatg
     4441 ccggcccgtc tcttcccgat ccgggtcaat gacgagcttt ttgccgccag cgacatgaca
     4501 aagcggatgc tccaggatgc cctggtttcg atgggcttga ccgaagcgct gaatttcaat
     4561 tttgtcctcg aatcggatct ggccgcgatg cagatcgatc cgcaatcctg tgtgacggtc
     4621 agcaatccga tgagtgcgga ccagaagtat ctccggccgg gtcttcttcc caacctgatc
     4681 gccaatgtcc ggacaaatct tgggagaggt gtccgcgata ttgcgctctt cgagatcgga
     4741 aagacctttg tccacgggga ttcggccccg gaggaacggc tttctctggg gatcattctc
     4801 tccgggaagg cggtttcttc gaactggatt gaaccgggcg gaagagaata cgattttttt
     4861 gacatcaaag gcatggccga ggctgtgttg gccagactcg ggatgacgga tctttcctgg
     4921 gagagaaatt ccgggtccgc ctggcatccc gggcgctcag ccatgataaa gaatgaaggg
     4981 actccaatcg ggaggattgg tgagctccat cctgcccggt gcaaggtcct tgacatccat
     5041 cagcgtgtcg ttatgatgga gattgacact gtgggcagga tgcacggtcg tggcgaaggg
     5101 ggaaccgttg tggaaccttc ccgttttccg agcctcttga gagatctggc ctttgtcgtt
     5161 ccggaagatg tgcagagcgg cgctctcatg agtaccatcc ggaaagctgc gcgtcatctg
     5221 gagatgctgc gcctggtcag tgtctggcgt ggtgaacaga tcgggaccgg aaacaagagc
     5281 ctggctttct cgctggagtt tgtctgccgt gaaaagactc ttgccgatga cgagatcgaa
     5341 tccattgtcc aaaacgtcat caaggaagcc ggtgcgcgac atggcgcccg cctgcgatga
     5401 gcggaggaaa gtctgtggaa ttgctgccgg ccgatgttct cgggaatgcc attgagcggc
     5461 tgatcgggca ttgccagtcc cttgaccggg atcggaccga tttggcccgg gcattgtccc
     5521 gggtccagga ggagtttgtc aatctccggg ccgagcatga gcggctcatt caggcccata
     5581 gagagcttca ggagcaccat aatgcgctgg aaaatgcgtg tgctgtcgcc gtcgagcgtc
     5641 tcgaacgtat ggtttcctct ctaggcaccg ggattcctct gaccccggta caggacttga
     5701 tgcaccagag tgccgaggtt gttgttcagg atgccgaagc aatccaggaa tccggagcac
     5761 cggttcggga agaggtcacg cagcaatccg gtctggcagt ggcggagcgt gtgatcaagc
     5821 cgctcaatat cggaaatttt gaacggatcg tgcgacaggc tggaggtgtt cttccagagc
     5881 cggtcgaatc cgtcatcgaa gccgaagatt caacccccga tccggtggtg attgaatctt
     5941 ctggttgtat ccgcgacaga ctcttttgat gcaggaagca ggggcgcgta tgggtgagat
     6001 cgtgacgatg catgtctttg gtcaggaata tgaactgcgg atcaacacgg agagcggacg
     6061 ggagcggctc gagattgtcg ccgagcttgt tgaccggaag atgaaggacg ttgcagcacg
     6121 ctatgtcagt ttgacgacca aggaggtcgc tgtactcgct gcactgaatc tcgcagatga
     6181 ttatattcaa ttacaggaaa gactctcatc cgttgcaatt gatccagttg ttgacgagga
     6241 ttcatcggtc cggatgctgg agtatgcagg acggattaat gcatatcttg gagggcaaac
     6301 ccgataatac tccctgcgct gttcgtgagc ctatgcacct cgtgagccac aagtcaatac
     6361 gaacgggatt ccaagatctg cgatgtgata tgtattccct cgcgggaaag atagagcatc
     6421 gtttgaggaa acccacgtaa caagagaggc ccatccaggg ctttggccac ggcagaggcg
     6481 gggttttttt atgcaaggta gagttgtgga gatcaacaac aggagaatag gatgaagatt
     6541 ttggaacgtg tatgtgcttt gaaaccgatg accaaggcat tggtcatcgc tgtcccggtt
     6601 tgtgtcgtgc tcggggtgct cgcgtttgca aggcttttca ataccgctga tgatgccctt
     6661 gcaacctgga ttggcaagga tggtacggag tactcgataa cacgggccga ggcatatatg
     6721 agcatgggtc agatgaagga tcgtatgctc caggacaagg cctaccagaa gaaccatttg
     6781 gacaatatgc tcttccagcg ccttcaggcg atggaagcca tcgacaacaa gctcaatctc
     6841 gatcccgggt atgtcaaacg gaccaatgag catatgacga acaacatgct cattcccgaa
     6901 ctgctccaga cattgtttcc acgggagagt cttgagtttt ctctggaggc ccgcaaggta
     6961 cgcatgatcc ttgtcaagag ggacgggttc aaccatatca gtgaaccgga tccgaaccgc
     7021 ttcaagagga tggcggatat ccagaagacg attaccaacc aggccaggct caagcaggag
     7081 atggagcgtc ttgaaaaggc aaccattaca aaggacgtca aaaaaaccga tgccgagatg
     7141 gcagcgcacg agaccaatat ccaggcaaag gcacagtccg ttctggcgat ggtgaaaaag
     7201 ccgggcgcag atttcgccga tattgccaaa cagaattccg atcatccatc cgcttccgaa
     7261 ggtgggcagg tcgggtacgt tcttccccgc agccggtcac actcacccca ggtcatggcg
     7321 gccattgcca agctgaaagt cggcgaaata tccggagtga tcaacacgca tgaaggcttt
     7381 gtgattgtca aggtcgatga gattgcgact gtgaccgaag tgaatcttgg tgagtactat
     7441 accgatgaag gcaaaaagaa ttttgccaag aacgaggcct ggttcgcttc ggtctggtct
     7501 ttgatcgaca agaccatcga agaccagaag ggaaaatcgg tctttgttta tcgtgagaat
     7561 cttgtctcca aagacagcaa tgcactggtc tttgaggtca aacatcccaa ggtgcagatg
     7621 aagataaccc gcggggatct tctgagaaaa atgggaaatg tccctccgcc gagacggtcc
     7681 atgtttgggg tcctggacgg gatggccgat ccggctggat actcggctga cgagatgtgg
     7741 aatttcttcg aatgggagat gtcttttccg attttgaaac tggcagcctg gctgaacggg
     7801 attgttgaat cggatgcctt ccaggccaag ctccgggatt cttcgtactc cgttctcgcc
     7861 agcatgcttt cggacaagag gagggagtcg gctacagtta ctgacgaaga gatatcggca
     7921 cagtatgaca gggccaaagc cagctatgtc aagcggcttc ccgggggggc aaccgaacaa
     7981 ctgtcactcg atgaagcgaa agagcagatc cggaacgagc tgaggaataa aaagatcaat
     8041 gattcattcc ataactggaa gaacgaactg ttcactcgct atcaggcaaa gatccacgaa
     8101 aacaggttcg aggtgatcaa gcctgagccg cccaaacaga acaatccgca gcaagccaac
     8161 ccgaaaaacg ggaacaagcc ggatgatgga aaaaaaaaca gcgggaaata agcagtagcg
     8221 tctggagttc ggtgcaggag tttctttttc ctgcaccgag cgccacctat tttaccggtc
     8281 tggccgacag cgtggaagca gaagtcctcg ccctcaagaa atcagggaag actgacgagg
     8341 cggttcgagc ggaaaacagg atgaacgggt ttctggtccg ggctttcggg gcggatcagg
     8401 cccgttcttc tttttgcagg gatgcggtct ccaggatgta caggcatggg cggatcgatc
     8461 tgatggggga atggatgcag cgggtggtct ggacccgcga ccgggatttc cggttcattc
     8521 atgcctggct ttccaatgtc aatccgaata ttgccggtga agcggtgaca gcattgggga
     8581 agagcggtga tccggcgatc tcggaagaat ttgcccggcg attggtcgat tcggaaagtc
     8641 tccccatgcg gttgcggctg atcaaggcgc tgggagaatt gggattgccg gggaccatgg
     8701 attcggtcct gtcggcatgt gattcaagac tgcaaaacag gaattatctt ccctttgccg
     8761 aggaaatggc gatcatcacg accatgggca ggatcgggga aaaactggag aatccgcaca
     8821 gggtgggccg gcttctcgct gaccgttggg acacgctggg gaccagaaag cttcagtctg
     8881 aagaattgct ttccctcagg acaaatatcc tctacgcact ctccagggcc ggccaagaag
     8941 agactggaag gatctaccgt tccatcctgg atcaaaatgc tccggggccg gttcttgatg
     9001 ctgtcatccg gtcagcagga cggacaagga tcgaggtggc gcgtgatgta cttccgcgtg
     9061 ctttgacgat ggaactgacc gaggatgttg aacacgattg tatcgagaag gcggcggaat
     9121 tttccggtct gggagacggc cgtgaattgt tatttccgac ggagtggctg gtcaagagac
     9181 tcgaaaggaa ccgcgatcgc agtgccatcc tgaaaatttt ggcaggccat ccccctgtat
     9241 tttcaaaaaa agtctttctc gacattctga agagtgacag agcgatcact ctccgcgaag
     9301 ccgatgtgat agcccgccat ctccaggagg gggtcgtccg ggactctgcc ggtcttcttg
     9361 ccggaaaaat ccgtgccaat cccgaagatg aattggtgcc tctctggatg caggtactgg
     9421 catcactcag ggttccggaa acgcgaatga tcctggtcga cctctcgagg gatggattgc
     9481 cgggtatccg gatccttgcc gagcagattc tggactcatg gaaaccgtga tgatggtttg
     9541 cttgggaaca ggccgggatg gtatcttgta aggatgatgg aatgaacaag gggcataata
     9601 tgagactgac actgctgttc tttctgggag gcctgatagt actggtcgct tgcggaaagg
     9661 acgacaaatt ttcggagtac aaggttgtcg tggggaatga ggccgagacg ttgatcgttc
     9721 ccgcacagga tgccatcagt atgccttttt ccggaggccc tgaagatatt cctgttgctg
     9781 ccgagcgcct gtatattgaa atgcgcaggc ttggacgctt ttttggtggc ccgctcagac
     9841 tggaatgcac ggtggaaccg gactggaccg caaaaaaatt gactggccgg atattgtttc
     9901 cgctggcacc cggagagggc gcaagggaat atgctgacag gccggagtct ccggtcaagc
     9961 cggaaatcgg tcgggtctcc gggggcagat attcgacaat tctgtttgat ggaccattgc
    10021 aggcccttga ggcaggccat ctcaagttgc gggatgctgt cggcaaggca agcggacagt
    10081 gtgttttcat gtttgaaggc acactcaagg agattgataa acgataccga gtgcgattga
    10141 tggcacggac cggagagtaa ccatgaagaa acatgtgttt gcaggagtat tggttctgat
    10201 gatggttccc ttggcgggat gtttcagtga gcccgaggca cgtctggaag aacgtgtcca
    10261 gcgtattgaa gtactgttgg agccgtccga aaaaactttg ttggattcag gcaagtattc
    10321 gctggctgcc gaaagtctcc ggaagagact ttcggaaaac aaggagctgg agcggcgcta
    10381 tcgtgatgtc atccaggaag agaatatcgg tttttttgat gttgaatccg ttctccgctt
    10441 ttaccatggg agcatgcgtg cccgggccag actcttccgt tttatcgcaa tgcttgctga
    10501 tcccgaggta acagctttca acgagaggcg ctttgccgat ttggcgagga gcctcgaagc
    10561 caggtttgcc ctggagagcg gactggaaga cctgtaccgc aaacagaccg ccccggtcct
    10621 tgatccggtc agggacgacg atattgcgcg ggagtacgcg ctggactccg ttgtcaagcc
    10681 gtttgcggtc gtgtacagcg tcatgaatcc gggagaaaga gaattgctcg taacggacaa
    10741 ggccggaaat gccgccggga atctgcgcgt cagaatgagt gccgatccgg cgttacaggg
    10801 ggccttgaca accatccggg tactgcttcc ggagactcgg tccatcgaag atccgggtcg
    10861 ggtcttttcg attgcggcag cacttgccgt gacgaaccgg gaagccaggc aggatctggt
    10921 caaaatcgcc gactggatca gtgccgcatc aaagaccggc aggcttgtca gcaagacctc
    10981 gatcggtacg gcattcccgg acgagatgac acgactggtg aatatcctgc ccaagccgga
    11041 tgtgaagaca aagcggttgt ttctgctcga gcgggagatc agctggtttt cacttgtcgt
    11101 cgacgaaaag atcaattttt acaactaaag agcggtgcga gagatgttca gccctggtgt
    11161 gtctcaattg gctacagggc gaccgtcgcc gccgatgaag ctttcctgcg gagagatgcg
    11221 gatttccccg aattgctcct cgaattgccc gatgttctgc ttgagggaat gcaggaggag
    11281 ttttgcgtgt ttgggagtca tgatgatcct ggagtgaacg cgggcgctgg gattgcccgg
    11341 caggaaggtg gcaaagtcga ggatgaactc gctgtcggag tgggcgatga tgacgaggtt
    11401 ggagtaggca ccctccccga tttttccttc cagttcgatc ttgatctcct gcatgttttt
    11461 ctgctgctgt ggtgacatga gcgcatcctc ttgtatgatg tccacaaagt gtggtatgaa
    11521 gagaatagcc tttcttctgg agcaggtcaa atggaacaca tgtcagtgcg tgccgccctt
    11581 ccgagggatg ccggcgcgat cagcgcgttg cttgggggat acgccgaacg gggcctgctt
    11641 ttgccacgtc cggtcgagga aatcctggag agtatccggg atttttctgt tctggtggat
    11701 ggagcagatg aagtcatcgg atgcgctgcc ctgcggattt atgatacggc tctggcggag
    11761 atccgttctt tggctgtccg cgaggaatac aaaaaacatg gggccgggcg gagactgatc
    11821 acggcttgtg aggacgatgc ccgtcgcaac ggaatttccc gggtttttgc cctgacctat
    11881 gtcccggagt ttttcgagag actggaattc acccgcattc ccaaggagac tctgccgcaa
    11941 aaaatctggc gggactgttt tcagtgcaag cattttccga actgcggtga aatcgcagtt
    12001 gtcaagacac tctcctcaaa ggcggagtaa atgctcgcta tcaaaaaacg tcacaagaca
    12061 ggtctggctc ctgggacaat ggtccatatc gggaagtatg agaaacttcc cgtcagggtc
    12121 cattcttttt tatggacgaa ggagcaatgc cgggaggaag ctggaattcc tgaaagcgag
    12181 actctggccg gactggcaga acggaacggg attctctggg tcaatgtgga cgggttgcat
    12241 gatgttgaaa aaatcggagc gattggttcc cggttttccc tccatcctct cgaactcgaa
    12301 gacatcgtca atacggagca gcgtccgaag atcgaggatt ttgatgatca tatatcggtc
    12361 atgctcaaga tgttgtcgta tgacacacag gcaaacaggg ttgaaagcga gcatgttaca
    12421 ctcgtcctgg cgaaaaacac gattctctcg tttcaggagg aacgtccggg agatgtcttt
    12481 ggtcccttgc gggacaggct gcgatccaat cgcgggaata tccgctgcca gcaaccggac
    12541 tattttttgt attccctgat ggacgccatc gtggacaatt attttgtctg tctggagaga
    12601 atcggggagc ggatcgacga aatcgaggac aggttcttcc ggaatgagtc gaaggacatg
    12661 atgggcgaga tccatgatct caagcgtgag ctcctctatc tcaggaaatc cgtttggcca
    12721 ctgcgggaca tcatcaggga ggtcctgcgc atgaaaaccg cgctggtctc ggagtcgacg
    12781 gaagcctatt tcagggacct gctggaccat gccatccaga tcatcgacaa cctggagctt
    12841 ttcagggaaa tgttgaacgg gctggcgaat ctctacatgt ccagtctgag catgcggctg
    12901 aacgagatca tgcgtgtgtt gacaatcatt tcgacgatat ttatccccct gacattcatc
    12961 acaggggtgt acgggatgaa cttcaagacc cttcccggct cgggatggga atatggatac
    13021 tgggccatct ggggtgtcat gctgagtgtc gctctcggga tgcttggctg gttcaaacac
    13081 cggaaatggt tttaaggctt ccccgtcccg gtctgcgttg gtgggcaaga aaataaaaag
    13141 cccccagcgc cgagtatctg gctgagggcg attcacaaaa aaggtcttgt ctcagagcgt
    13201 gagccccatc tccttgacga catcccggat cgcagcaaga agatctcgac cgtcgttggt
    13261 attcatccgt ccgatatggc cgatgcggaa gcactcggcg ttgctgacct ttcccgggta
    13321 gatcacatat ccccggtcat tcagtttgcc gtagaatgtt gcaaaatcga attttggatc
    13381 agcaggatag taaaaagagg tgatgatatt gccctggagc gagtcgggca gaaacggctt
    13441 gaagccgagg tcccgcatgc cttgaatcat gaaatcatga ttggcccgat accgggctgc
    13501 tctggcaggg atgcctccct cggcctcgag ctcaagcagg gcctgatgga aggcgagcag
    13561 ggagtgggtc gggggggtga accggaactg gccgtctttt tcaaggcctt cccattgtgc
    13621 atacagatcc agggtcaggg ttcgtgcccg gccacggcat tcgtcgaggg ccgatttctt
    13681 ggcgatgatg aaggcaaatc ccgggacgcc ttcgacgcat ttgttggatg aggagacgat
    13741 gaagtcgata tttccggacg ggacatcgag ctcgacgcca ccaaaggccg acatcgaatc
    13801 aacgaaatac cgttttccgg cccgggtgac gacgcgcccg atggcattga tatcattgat
    13861 gatgccggtc gttgtttcac aatggacaat ggccacatgg gtgatcgagg ggtctttctt
    13921 gagagcctcc tcgacccggg ccggatcgac aagcgtgtct tcgccaaaat cgatcgagtc
    13981 gactgctatg gaatgaacct tggcgatggt catgatgcgc ctgccgtagg ctccattgac
    14041 aaggacaagg agtttcccgt ctttggggat ggatgatgaa atgactgctt cgacgctgaa
    14101 agtgccactg ccctgcatga tgatgcattc gtattcaccc ttttttgtct gggccaagtc
    14161 gagcaaacgg ttgcgaatat cgcggacaac accgataaag gcataatcgc gggacccaag
    14221 atcccgcaac atggcctgtt tgactgtggg actggtcgtc agaggacccg gggtaaaaag
    14281 tattttgtct ttccatgcgg ggatggattt ttccattctt tttgctcctt gtacgattcc
    14341 gtagacagtt tttgaaataa tactccggag gcggggacgg gtggtaaaaa aggattgccg
    14401 ggacacgggc cagtttgtac cttgtaagga cacggcggca tagccaagtg gtaaggcagc
    14461 ggtctgcaaa accgttatac atcggttcga atccgattgc cgcctcaagt cttcttcccg
    14521 accgggatgc acgatcagct ggagtggcgg aattggcaga cgcacgggac ttaaaatccc
    14581 gggaaccgta aggttcgtgc cggttcgatc ccggccttca gcagaagcag ttttccggtt
    14641 gcggttggcc cggttccgga tcaaaaaaat tcttgatagt acctgagatg gctgtgcgtt
    14701 cactccgaga ggcgcgatgc attgattttt ggatatctcc aggacgccgt tccatgcttt
    14761 gtgcgatttc aagctacgct gaaacagacc gacctggtga gccggcttgt cccgagacgc
    14821 gtttgtgacc gatgtcgtgt ggaaaaacaa aaaagtcacg cgtgtgttcg cgtgacttgc
    14881 gtatgccgga ggccggaatc gaaccggcac ggggtcgccc ccgctggatt ttgagtccag
    14941 tgcgtctacc agtttcacca ctccggcgac aaaatagaat ctacccagcc ggagcgccat
    15001 tcgtcaagta ttcacatgtc tcacgcaatt tttcgggtgg tgctcgtttt cctgtcggat
    15061 gcggttggtg cagtgagaac acgaagctct ggtacctcgc cgagagaaga tcacacttga
    15121 aaagccagtg agttcagcgt agaatgcaat ttcaagagcg tgagtcggga gacgggaaat
    15181 gaaggttgaa tacatcaatc catttgtggt ggccacacag agtgtgtttc gggcgatgat
    15241 gaatgttgaa gcagtggcgg gcaagccctt cctgttcgat cctgtcactg gaagccatgc
    15301 ctatgatatt tcggggatta tcggtctttc cggcgagacg accggtgccg ttgtcctttc
    15361 gttcaccaaa ctctgtgccc tcaagtcggt ttccgcgttt tcagggatgg agatcaagat
    15421 ctttgaccag accgttgtcg atgccatcgg cgagttgatc aatatcatcg ccgggaatgc
    15481 caaaaaagat ctgacagact tcaagttgaa catttcactc ccctcggttg tgacaggcca
    15541 ttcacacaga ctgaactggc cgacaggcgt gcccgtgatc atgatcccct tcacttccga
    15601 gctcggcgaa ttttcgatca gtgtctcgtt gcgggaaccg tccaaacgct gatctctcgg
    15661 tccggccttt gtctccgggt gaagtgttgc tgtacgcggt gcgtcctgac tattatcaag
    15721 agacagcatg tacactgttt acggaagact ggagacccgg gccatggccg aaagagacta
    15781 caaattcacc gccattgaga agaagtggca gacctgttgg gatgagcaga agacattcaa
    15841 agtaaccgag gacccttcgg ttcccgagga aaagcggttt tattgcctgg acatgtttcc
    15901 ctatccgtcg ggagccggtc tccatgtcgg tcatcccgaa gggtatacgg cgacagacat
    15961 catctgccgc tacaaacgga tgcgtggcta tgcggttttg catcccatgg gatgggatgc
    16021 cttcgggttg ccggccgaaa actatgcgat caagacgggg acgcatcccc ggacatcgac
    16081 aaaccggaat attgacaatt tcaggcgcca gatcaaggca cttggtttct cgtatgactg
    16141 ggatcgggaa atcagtacct gcaatgaaga ctattttcgt tggacgcaat ggattttcct
    16201 gcaactcttc aaggcagggt tggcctatca ggccgttgtt ccgatcaact tctgcccttc
    16261 atgccggacc ggtctggcca acgaagaagt caaggacggc aagtgtgatc gctgccaaag
    16321 tctcgttgaa cggaaggaca tgaagcagtg gatgctcagg attacggcct acgcagaccg
    16381 cctgctctcc gatctggatc ttttggactg gccggagccg atcaaactca tgcagcgaaa
    16441 ctggatcggg aagtctacgg gggccgaggt cgactttgcg attgaaggct cctcaaaaag
    16501 tctgcgtgtg ttcacgacca ggccggatac cctgttcgga gcaacctata tgtgtatcgc
    16561 gcccgaacac ccgctcgttg ccgaattgac caccgaagcg cagcgggagg ctgtcaggaa
    16621 ctacgtcagt tcggcaaaga acaagagcga tcttgagcgg actgatctcg cgaaggacaa
    16681 aaccggtgtt ttttcaggtt cgcatgcgat caatccggtc aatggtgaga agattccggt
    16741 atggattgcc gattatgttc tgatttccta tggaacaggc gccatcatgg cggttcccgc
    16801 acatgacgag agagatttcg cgtttgcgaa aaaattcggg ctccccatta tcgaggttgt
    16861 cagcgtcgac gggaaggcgc acggtgagtt gacggaagcg ttgacggttg acggtattgc
    16921 catcaattcc ggtgcgttta cgggcatgaa gacccccgag gtcaaacaaa agatcacggc
    16981 ctggctctct gacgaaggca aggggaaaga atcggttcag tacaaactca gggattgggt
    17041 tttctcgagg cagcgctact ggggtgagcc gatcccggtt gtgcattgcc agaaatgcgg
    17101 agtggttcct ctgccggaat cggcccttcc tctgacactc cccgaagtca cgtcctatca
    17161 gccgactgga accggggaat ccccgcttgc ggctatggag gactgggtgc aaacaacgtg
    17221 tccggcctgc ggcggacgtg catggcgaga gacggatacg atgccccagt gggccggttc
    17281 ctgctggtat tacatccggt ttcttgaccc gaagaatacg gaggcctttg cctcgcccga
    17341 ggcgatcaag tactggcttc ccgttgatct ctatgtcgga ggagccgagc acgctgtgct
    17401 gcacctgctc tattcccgat tttggcacaa gtttttgtac gatcaggggt tcttgccggg
    17461 gccggagcct tttacacggt tggtcaatca gggcctgatt ctcggtgagg acgggcagaa
    17521 gatgtcgaag tcgttgggaa atgtgatcaa tccggatgat gtgatcgcgg aacatggtgc
    17581 ggatgcgatg cggctctacg agatgttcat gggaccgctg gaaatgtcca agccatggaa
    17641 taccaacggg atcctcggaa tccggcgttt tctcgagagg gcttggcgtc tcttcgaaaa
    17701 agaaatcgtc tccggtggtt cctccggcga gattgaacga ctcaggcaca agacaatccg
    17761 gaaagtaacg gaggatcttg atgcgatggc attcaacacg gccatctccg ccctgatggt
    17821 gtatgtcact gaactcggaa aactggaaag cctttcccgg attgacggcg agaccttggc
    17881 acttctcttg gccccctttg cgccccacct tggcgaggaa ctttgggagc ggctcggaca
    17941 tggacagacg atcgcctatg aaccctggcc tgttttcgat tcggacaaga cacaggatga
    18001 taccatcgag atagttttcc agatcaacgg gaaggttcgc gacaaggttc aggttcacgc
    18061 atcggtctcc gggccggaga tggaaagccg tgcgcgttcc agtgacaaga tccagccctg
    18121 gcttgaaggc aaggagatcc tcaaggtgat caccgttccc ggaaaactgg tgaatatcgt
    18181 catcaaagga tgagtgctgc tgtcattccc ataaacgcac aaccgtcagt ggtgtcccgg
    18241 atacccatga cggttgtatg attttgcgat gtatttccgg gaaggaccgg attgcatcag
    18301 ggcgtcagga gagatgtttc ccctgtgcca gatactcctg gacatattca cgaacagcct
    18361 cttcaagagg cgtacagggc gagtcatatc cgacagagcg gagtcgggat atatcggcgc
    18421 aggtgtagta ttggtagcgg tcccggaggt ggaggggcat gtcgatatac gtgatggatg
    18481 ggggctttcc catggcgtca aagacagcag tgaggagctg gttgaaactg gatgccgtcc
    18541 cgctcccgac atcgaaaatc ccgttgatat cggggtggtc caagaaaaag aggataacct
    18601 tggtgacatc cttgagccag acaaaatcgc ggcgttgttc tccgtcggca tagccttcgc
    18661 gatgtgagcg gaagagcgga tattccgagc cggagttcag tgcggaccag gctttgagga
    18721 cgacactgcg catgtcgccc ttgtggtatt cgttcggccc gaaaacgttg aagaacttca
    18781 ggccggccat tttatggagc cagccccggt tgatggcgtg gagatcaaag gcatgtttgg
    18841 aatacccgta catattgagg ggtttgagag accgagcgga ctctgggtcg tcatgatacc
    18901 ccgtcccgcc gtcgccatag gtcgccgcac tggacgcata gaccaggcga atgtcatgtt
    18961 tgagacacca ctcagccagc gaaacactcc aggcgtagtt gttggacatg agatagtccg
    19021 cgtcggtttc tgtggtcgaa ctgcaggctc ccagatggac cacggcttca atcccggctg
    19081 gagtgaatgt cccggatgtg attccgcgga gaaagtcgtc cggctggata aaatcacgat
    19141 actgcaacga gacgaggttc ttccatttct cgcctttccc gagccggtcg acaatcagga
    19201 tgtcctgtct tccgcgtgta ttgagggccc agactgtggc actgccgatg cagccggctc
    19261 ctccggtcac gatgatcatc gttccatccc cctgccaccg ccgctccatg taccgagcag
    19321 caattcatac aaactgcgga ttctggaact gccagggata cgcagatgtt tttgttttcg
    19381 tgttttgtgc gccaggagcg tgcggcctcc gatatcctgt ctgcaggagt aggtgccgct
    19441 tcgaatcacg ggaagcgccg aaaggtaagc cgagtagatg gtttcatcca gagtccgtcg
    19501 tgaagagacg agggagaatt tgagggaaga gtccgaagcg gatggaatga tgaaggtccc
    19561 ggccggtgcc tcaggaagtc tgccttcacc ggaaaaaacg atggcgatgg cgttgtgtga
    19621 tttgactggt ggctcaaaga cctccagttc atcacggagc gctgccaggc aggccgggat
    19681 caggtattcc ttcgtggcga tcaggatggc atcgctctcc ggggagagga gaccgaaaaa
    19741 gatattctgc aggaaggggt gtccgtcaga ggaaatgtgg agcctgagcg tgacaaaacc
    19801 gcgccagata atcccttcac gacgcaaggc cccgtgaagt cggcccagaa tcagggtgcg
    19861 gatccggcgc aggatcgggt cattatcgga aaaggccgga gtaaaagctc cagaacccga
    19921 tgtggcgagt ccggtgtcgt tatcctcggc ggcaatatcg agctctgcac atcccagcaa
    19981 aaagagcgtg gtccggtcgc agagggcgat gacggaatac tcacgggcca tttcaccgca
    20041 gagaatcttg accggaatga catcattgaa tatccccgga tgtgcggaga gaaaatcgga
    20101 aagagcgggg atttcgttga aaaccggatt cagatccgcc tggagtcgcc cggcttcaat
    20161 ccgccatggg cccttgtgtg tcgatgcaaa ctggacacct tgatcaacag aggaaatttc
    20221 catccatgga aggaggggaa tacggtagcg ggccaacaat gccagcaaag actcatgatc
    20281 tagggtggat accctgtttc cgtatccgaa gacagggaca gcatgtgcga tcaggcgatt
    20341 ggtcgtatcg tcatcgagcg cgagtgggga tgtgagaaat acaagtgagt cggacagatt
    20401 ctggaggagt gatgtgaaat cgggcagctt cgctgccggg aggggtgtgg cctgtggatc
    20461 cgtctggccg gatgaaacag tgtgaaagat tgttccgcat gacccgcagt ctgcgagggc
    20521 cgtgatcagg gaggatgctg gggcgccgtc gcccgcgacc aggacggtct gaagtcggtt
    20581 ggtggcgggg atctggacct cctcgagtgt ttcctgaagc gtgctgaggg tggtgttcat
    20641 ggtatcgagc tgggaattga gaaaggaaat ccgcagactg ctgtcgacgg acatttgttg
    20701 caggtttcgt gcaacctccc ccagttcgtt cagggattga cggatgttga cgaaacgcgc
    20761 cacgagggat tcgcgcaggg cagagacatc attgcggatg acaagaggaa gtgacgccag
    20821 ttcatcggga tcctggctca agtcaccgct ttcgaaacgg gtgagaatcc tgtggagggc
    20881 atccgcttcg cccttgatgg tccgtgaaaa aaagatgcgt cggaccagac cgaagagcag
    20941 gccggccaaa aggatgacag ccagaatccc cccaccgctc gtgagaagga atccggcaaa
    21001 actgtcaaaa ccgaacatcc tcttgaggac tctggcatcg gctactgcct ggaggacgac
    21061 ataaaagaga atcaggaatc cagccagaga aaacagcgca atcaccgctg tctggaccag
    21121 gagactcttg cgcagtttcg gcagcactcg ttacccttta ttgattgaat cccgggacaa
    21181 ccagtccgga tcagtgaatc ggaatgcgat acgttcttgc ctgcgcgcct tggtagccgt
    21241 gtcccaagcc atacagtcga acgcggagga aggagccagg tgtgatgttc aggccccgga
    21301 ttccggtttc ccagtatcca tagacaacct gtttcgggat ctcgatacgg aagacacgcc
    21361 ccatcccggg atcgtccacc ggcaatgacg tggccaggaa gtagagccca ttggcgggac
    21421 cgaggagagt tccacaccag aggatttctg gcttgttcgt attgccgaca ggggtgatgg
    21481 agcggagcgc ccaggtgatg cgcccttgag cggtaacaat tgtcggccca aggatgacaa
    21541 cggaggaaag caggggttgt tcacggatct tgagtgtcca gccgtttgca ttggaactca
    21601 aggaatagag cgaaccaggc agttcgtcct cgatcagggg aatctcccga ccggcattcc
    21661 atgggatggt atctccggac tgatgcattg caatccgggt aatgaccgga gcgggatgcc
    21721 ggtgacggta tgtaatccgg ggatggcgga aatcacggga cgatcctggg gcaaggatgt
    21781 cgtgattttc agaagagatc aaggcatgtt caggagagtc gctgatgatg gcctggccgg
    21841 ccaacactcc gctgaaggca aaaacgaaaa tggaaacaag cgtggatatc cgtgtcacga
    21901 tgtcctccct ctatgccggg tgtttgcaat cccttgcagc cgacagtaca atagtagcgt
    21961 gttggagcgt tcttctcaag accaattatt gatgccggat tcgaatgatt ccggagtctg
    22021 gagtacaaac catggaagtc tgtttcaccg catcggttcg gcctttggct tcagcgcggg
    22081 gaatgtaccg gttttccttc gccgcatcac tggtgtttgc ccttttggcc gtcctcgggc
    22141 ttgagagcat cgggtccggg ttttttgtgt tcaccatggt cgcactctgg tttttgggag
    22201 tcggctgctg gcaggtacgt caggccaggc ggctcaagtc cagcgtaatc ctgattgatg
    22261 acgatggcct cctggcgagc atttctgggc gggagcagtt ccatcctgcc agcgaggttt
    22321 gtgcgggaca cgggagcatc gcccggtcgg ccggttttgg aaaacagtac cggatttgcc
    22381 gggctgtcag gatctgtcag agtgttgttg tggccgatat cgagacagcg gtgcgcagcc
    22441 ggctacccga gcagcacgtt ttttccgggg atgttctccc cttggatgcc gccgagtacg
    22501 atcgttttct ggctaccctc aaggagcgtg gctgggacag gccctgcatg gaacggtccg
    22561 agacccctga gcgcgggcgg atatccgtct tgcgggtggt ctatgcgagc ggcgttttgc
    22621 tctgcatcct tggctggatt tttttggaca atacacgcct ggccctggcc tttttattgg
    22681 gcgggcttgg tttggccggt cttttggtga acagtctcta tacacgcaat gggccatcaa
    22741 cgcctggcga ggaacggatg tcctgagcgt tcgtgttccc ggcacttgcc gcacggcccg
    22801 gagggttgta ctttgatcgt gtgccgttcc tccggggcag gggatcgcgg ccacgtcgct
    22861 tcttgcgccg gtgccgagga aagtccggac tccagggaac aaaaatacgc cgggtaacgc
    22921 ccgggcattc tgtgctcttc cgcaagggag ggtgcatggt gacggaaagc gcaacagaaa
    22981 gcaaaccgcc cagggtcgta cgaccagggg taagggtgaa agggtggggt aagagcccac
    23041 cgcggctctg gcaacagggt cggcacggca agccccgtat ggagcaagtt caagcagcag
    23101 gccgcgttcc cgaatggcct gcgggtagaa tgcacccagc gagcggggag acccgccgcg
    23161 cagacagatg atccccctcg acagaatccg gcttatcgct cccgggtgga cggcacaaat
    23221 ttttttgaaa tattcttgac gcgcggttca aatttgccta tgttttcttc ggcttaataa
    23281 gccttatatc caattggagg aattatccta tgaagaagat cttcgctatc gctctcgtcg
    23341 ctgtcatgag cctcgttgtt gttgcttgca agaagggcga tgcccaggcc ccggccacca
    23401 atgtcgagac caaggttgag aaggttgatg tcaaggctga caccaacgcc aagaccgaag
    23461 tcaagaagga cgagaaggct ggcaagtaat tcttgcgatc ttcggatctc cgaaagccat
    23521 cctgcggggt ggcttttttt tatttgtgca gccagtctgg atatcaatga aagtgcggtt
    23581 ggggtgatac acttggctcg atctgaggga tgtgatcatc tgcggtgccc gcaaacagca
    23641 gatatcacca cgagtcgggc ttggaccaat aggttacggc tgaccacggc gacaccatgg
    23701 tgcgcagcgt atcgccagtt ttgagggtga catctccctc ctcaagcgga gagtgagatg
    23761 ctgatcgtgc aggggcccga atttcgtctt gagagcgagt actatggtgc agaaatttct
    23821 gtattccatg tcgattcagc gatacactgc ccggttctca ccgcgcagga tggaggacta
    23881 catgcccgta ggtcctcaac aagtgccatg gtgcgcagtt tacggccaat agagagggtc
    23941 aagcctgccc ttctcaagcg tagagggcga tattgatcgt aaaaggcccg aattccgtct
    24001 tgagagcgag cactatggtg cgtagatggg ggtggatcag ctgcagatcc tgcccgaggt
    24061 agaattttgg cggtgagatg gtgcagttga tcccgttttg ttccaggagg gtgatggccc
    24121 gggcagtgat ctggttcccc agctccagga gcgctgaatg caggagcttg cggaaatcac
    24181 ctgcctccag catcgagagg tcgagctgct tttccaccat gcgttgggag agtctggccg
    24241 cgacgccctt ggaaaattcg tagacgatct tgccgtggat ctggccgctg atatcgagac
    24301 tcattccggt gccggctggc gtgaattttc cctgttgaac cgagacggca tcctttgcga
    24361 ccgagaggcc cgtattttca cggacaatct ggacagctgc aatgatgaag gggttgactg
    24421 tacgtgcttc catggcagga cgatccctcc gtgtcgactc gggtgtggtt ggtcgttgct
    24481 tcaggatgac ggccggcatg gtgtctccct ctccatgata cgatagtgac agaagccccg
    24541 caagaagggc cagtccgtag tgtttatcgg tagattgtgg gtatcagtca agagtgactc
    24601 gccggaggtg gcaagagatg gcagacggaa cgcagccagg ttccctcgag aaacataggg
    24661 agtgggttgc ccgggtcttt gccagtgggc ggattcctca cggaattctt ctggccggcg
    24721 cgccgggttc cggaaaactg accctggctc tcgagatggc ccgtcggttt ctctgtcacg
    24781 ccccggaccc gggatctcgg ccttgcggga cctgcatcgc ctgtcgggag atgttgaccc
    24841 tgcgctaccc ggatctgatc ctgttttctt cacgggatgt tgtccctgag gtacgcgccc
    24901 tgacggcgct cgcttcccgg gcggttgaga cccctgcggg ccgtgacagg ctgattcagg
    24961 ctgtgcgccg gttgttttat cgagtcgtca atcgggtcaa cggcgggtat t
//
LOCUS       DAOSHE010000002        21063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_2033_length_21063_cov_5.490527, whole genome shotgun sequence.
ACCESSION   DAOSHE010000002 DAOSHE010000000
VERSION     DAOSHE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21063
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_21063_cov_5.490527"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLD82_00155"
     CDS             <1..186
                     /locus_tag="PLD82_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63830.1"
                     /translation="SQVLSLLTSEVRQALLAFVNKHGKIQVHDRAVLWVHHGMDTSAV
                     PGVVASMKELARALGPY"
     gene            complement(384..878)
                     /locus_tag="PLD82_00160"
     CDS             complement(384..878)
                     /locus_tag="PLD82_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63831.1"
                     /translation="MRQSPEQDAIEKRLGPGKTSRDGFLGTDTRHLGEIIVEDDLSVK
                     RLDLTHAAIVDRMRFFRDAAEEGFGGVVMVPPHFEVRSDSVRGKISCPFGHQGGGFAK
                     NTVIVENTRLNKQVMYTDLTIHLIEAHGFYQGRGAYFRTDPAVLAEVLEVERLAPPVI
                     PAGE"
     gene            complement(958..2091)
                     /locus_tag="PLD82_00165"
     CDS             complement(958..2091)
                     /locus_tag="PLD82_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOG63832.1"
                     /translation="MSPLETFFVSVGVITAIATGLAFLVVLAEMTIGHYGRMKIDINN
                     GEKLLDVEGGQSLLTTLKEEGIFIPSACGGRGSCGLCTLKVTSGGGDYLPTELAWIKP
                     ESREKGIRLSCQIKVKRDMSIEVPSRLFSIKEFSGQVEYIEDLTHDIKRVRIRLTEPA
                     DISYKAGQFMQLAVPEYELSSEQVSRAYSMSSAPHETGLVEFMIRLVPNGICTTWVHR
                     YLKEGDRVSLTGPFGEFFLQETSREIICIAGGSGMAPIRSILADIEHRAHAQPEIAAR
                     RCRFFFGAVSRRDLFVLDEMRRLEASLPAFTFIPALSAPKPEDAWQGESGLITDVVRK
                     HLGTTGSEVEAYLCGSPGMIKACISVLNECGIPVDRIYYDSFG"
     gene            complement(2110..2700)
                     /locus_tag="PLD82_00170"
     CDS             complement(2110..2700)
                     /locus_tag="PLD82_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOG63833.1"
                     /translation="MNEISVLSIFLAAVFTNNILLTNYLGMCSFLAVSSEVKASAGLG
                     IAVTFVMAVTSVLNWLVEQYILVPLDLVYMRYIVYIVVIAAFVQLVEMIIERVSDSLY
                     AALGIFLPLITVNCAILGATLFIAIREYTLPQAAAYGAGGGLGWAMAIVAMAGIRQKM
                     ARSRVPRGLSGAGISLVITGIMALAFIGFSGMIRVN"
     gene            complement(2704..3324)
                     /locus_tag="PLD82_00175"
     CDS             complement(2704..3324)
                     /locus_tag="PLD82_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOG63834.1"
                     /translation="MTLAKPRMGRVIFDGIFANNPVFVQVLGICSTLAVTNVFENTLV
                     MCIGLVFTTGLSNFSVSLLRSLIPGRIRMIVETLVIASWVIVFDIIIKATLPEISKAL
                     GAYVGLIITNCIVMGRAEAFALANKPLISMADGIANGLGYTWVLLAVAFVRELLGSGS
                     IFGVRVLGEWWTNWNIMVMAPGGFFVLAILIWIVRGRMLRSKEGVR"
     gene            complement(3321..4064)
                     /locus_tag="PLD82_00180"
     CDS             complement(3321..4064)
                     /locus_tag="PLD82_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG63835.1"
                     /translation="MKFSSKRRERIFTVFFMFAISVFCISILTGVYLASRDRIRVNQT
                     LASKRALLAAARMPVPDDQRTLDAIFSNQVKAYTNAGGRAIYSIRQGASSDPSSGRRQ
                     LLGFVFEGRGQGLWGGIRAWVGVRADRKHLMSLAITEHSETPGLGARIDEPWFGKQFS
                     GKTGPLTARPEKTASSLATEFDGITGATATIDGVRSMLNTVLSNAPGDIAAIARTDAD
                     LSNAVFLYDAHSATNKNPGGIPRKEEAGP"
     gene            complement(4061..5077)
                     /locus_tag="PLD82_00185"
     CDS             complement(4061..5077)
                     /locus_tag="PLD82_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG63836.1"
                     /translation="MDTVKPLPWIMKQAPMRRMLLALSPLVLAAIWLYGWKSLAMVCI
                     AATVGWIVERLMLRPYHEPVSQAVFVTTTIFALSLPPTIPLWMVALGSIVAVLFGKMV
                     FGGFGRNVFNPAMVGRAFLYVSFGQAMTGVWALPLTGFLGNFPQAASAAAGTHGLFPA
                     GFGAWSPGVDAITSATPLASAAPSRIDLLVGTVPGSLGESSAILIGLAAIYLIWTKTA
                     NWRIIVPCLVGFMATWIPLWAAEVTTRSGILLPDPLVGLTAGGVLFGIVFIATDPVSA
                     AVTNGGRWIYGVIIGASTVLIRVFSVWPEGMMFAILLGNMFAPIIDWVIKNRKARPAP
                     GATP"
     gene            complement(5110..5784)
                     /locus_tag="PLD82_00190"
     CDS             complement(5110..5784)
                     /locus_tag="PLD82_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG63837.1"
                     /translation="MEPFEHRNKEYADRLLVLERACRGARRRLIWLAHGCGLDAFSAE
                     DVVQECFLSVTRSIHRLDCSGSLVPYLEKAVLRAARRCARRMGRQRYFEIPDADPVLC
                     DTGREYPDASLPGWESGLGILACVSTMEREILLARYRDHIRVDELAGMFGVSRRTCLR
                     RINQAKNSLAKVLALDAEGEARDVSRQSALKDVRDSGMRILEGCESPVGMSDADSSPG
                     RRYRDL"
     gene            complement(5849..6736)
                     /gene="rlmB"
                     /locus_tag="PLD82_00195"
     CDS             complement(5849..6736)
                     /gene="rlmB"
                     /locus_tag="PLD82_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG63838.1"
                     /translation="MKKTLIYGKNAVLEYLKTCRRPVGGRLLLARGNEGGAGKLMQDL
                     AVKLGIVCLSGPRRELDQLYPGLVHQGFVLELGQKGKAPGRVPDGRAQARPPKAPRPA
                     VEAREDRETEEEEIRLEPLPEDIVHWDWERELAAAVERGEWPRVLVLDHVQDPGNLGA
                     VVRSAAQFGVSCVIIPKDGASGMTPVARKAACGGDCHVPVITETNLVRVLERLKEMGF
                     WSAAAVGSDGKPVREFAFDSPFVVVLGSEGAGIRRLLLERCDWKLTIPMLPRLDSLNV
                     SVSAGIILHEMFSKRKILE"
     gene            complement(6733..8157)
                     /gene="cysS"
                     /locus_tag="PLD82_00200"
     CDS             complement(6733..8157)
                     /gene="cysS"
                     /locus_tag="PLD82_00200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG63839.1"
                     /translation="MAVQLYNTMTRRVEEFSPLVPGKVTMYTCGPTVYDYAHIGNFRA
                     YIFEDLLHRVLRFAGYDVTQVMNLTDVDDKTIRNSIAKGMALDEYTGVYKQAFFEDIK
                     ALGITPAAVYPEATKHVPEMISLIRNLLDKGFAYQKEGSVYFSIERYREYGKLAHLDR
                     EGLVAGASGRVDSDEYEKDHVSDFVLWKGYSDKDGDVVWDSPFGRGRPGWHIECSAMS
                     MKYLGETMDIHTGGIDNMFPHHENEIAQSEAASGKPFVRTWLHCAWLLVDGEKMSKSK
                     GNFFTFRDLVSKGWSPKVIRYLLISTHYRKTLNFSEQGLEASKAALARLDTFLADVAR
                     FSGPDADGNNIPSGQAAAVGAILESMLGGFTAAIEDDLNISEAMAAVFPVLPKIREHF
                     PLGKESADAVLRSFKRLDEVLNVLDFGSGESLDDEIEAMIAERNAARKAKNFAESDRI
                     RDELAKKGIILKDTPQGTVWEKHT"
     gene            complement(8456..9811)
                     /locus_tag="PLD82_00205"
     CDS             complement(8456..9811)
                     /locus_tag="PLD82_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG63840.1"
                     /translation="MSKILILDDEAEFCDKMAQMLRLRNYSVTAVYDTVAARKALEAE
                     SFDIILLDVFLQDGSGVEMLGYIRKRWPETAIIMMTGMEDVRTAIECIRLGAEEYFLK
                     PVHNDELVFAIEKSLKTRRQQSELTMYRDSGNLPIIGESQSIRDVRALIERAAANPDV
                     TVLVTGETGTGKELVARHVHMHSRRSQEAFIPINCSSIPESLIESEFFGHEKGAFTGA
                     ERQQKGVFERANGGTVFLDEIGDMEVRLQTSLLRFLETRRVHRVGGGEVDVDVRIVCA
                     TNKDLQAEVDQHRFRKDLYFRINVLNIHLPPLRERVEDIVLLTRHFIGKQAHSHREYR
                     ITEAGKKRLERYPWPGNIRELKNVVERETILAKGADLDFVQLEHGLMDMPEKCRTLDD
                     GGLHLVIGYESPISLDAIEKRVIEGALEHCNGNVSRAAEVLQLGRSSLRSKMKRLGIG
                     Q"
     gene            complement(9815..10417)
                     /gene="cyaB"
                     /locus_tag="PLD82_00210"
     CDS             complement(9815..10417)
                     /gene="cyaB"
                     /locus_tag="PLD82_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOG63841.1"
                     /translation="MNMAWEVEIKAHVKDAQGIETALGARYGRGVPFDKRDVYFESVA
                     NPGSTAFRLRNDGAGDGLPGPDVVTIKQKGMRDGVEVNNEVEFTVDSADAFHAFAQSL
                     GYRVAIEKRKRGHVWEVEPGVHIELAEVSGLGWFVEIEALLDGQGEIPAAEAQVRSLL
                     QSLGVAETDIEPRYYTSLLRLLKAGSGLEAHQGNADGRKR"
     gene            complement(10414..10962)
                     /locus_tag="PLD82_00215"
     CDS             complement(10414..10962)
                     /locus_tag="PLD82_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63842.1"
                     /translation="MMLSQGTPMLLAGDEFLRTQGGNNNAYCQDNEISWVDWSLADRE
                     TEQVRFVRDMIRLRKRFRSLRRETFFSGQPEEGSPIPDISWHGREPGKPEFESGIPLL
                     GCMLAGKGMEPAIYMLCNATGRPQRCVLPTSPDGGWWIKVDTAQGLVHPDGGEPVCAG
                     GILRVRANSVLVLLSRSLAGGT"
     gene            complement(10922..12502)
                     /locus_tag="PLD82_00220"
     CDS             complement(10922..12502)
                     /locus_tag="PLD82_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HOG63843.1"
                     /translation="MIRTCEVSGVSLRVYGGHPFPFGASRTEAGINFSVVSAGARSMS
                     IVFFTTGVQEPWFELALDPLQNRTGDVWHALVHGLPENIRYGYRADGPRDPANGLLFD
                     GSVVLADPWAMALTGGAIWGRPLVREGSATHHGAYLRRCFLPEKEFDWQGDRPLNRPL
                     SETVIYELHVRGYTVHHSAAVVAPGTYRGLTEKIPWLVSLGVTAVELMPVNEFDEEAG
                     VRLNPETGERLVNFWGYNTCAFFAPKASYAASALQGGQIHEFREMVRALHRAGIEVIL
                     DVVFNHTGEGGTGGPVISFKGLDNNIYYMLDEQGGYRNYSGCGNTMNCNHPRVRRLIM
                     DCLHYWVSEMHVDGFRFDLASVLGRDSQGNVLVNPPVLEEIAQDPGLASTKIIAEAWD
                     AAGLYQVGSFPAWGRWAEWNGRYRDDLRRFLRGDAGSSSALATRIAGSSDLYADDGRS
                     PQHSINFITCHDGFTLNDLFSHERKHNLGNGEDDRLQLRCGGRDRPPGCLAAQAETGK
                     KCGCHHDALPGNPDAPRR"
     gene            complement(12542..12958)
                     /locus_tag="PLD82_00225"
     CDS             complement(12542..12958)
                     /locus_tag="PLD82_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63844.1"
                     /translation="MRASERFEIEILLTGVRDVIPTARWSWDDARQTVSVRLGSDDEK
                     RVRKQLDRTFLFSCRKKRKTGSSPVAGLILQMHGSLRDGQQLWLSDPGCASGTYALSK
                     PWVDGENLTLVLGVWSGEDQSDRVLISSIRKIFGVH"
     gene            complement(12961..13713)
                     /locus_tag="PLD82_00230"
     CDS             complement(12961..13713)
                     /locus_tag="PLD82_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63845.1"
                     /translation="MSTVKITPSRGVKKTAEVAVRVQGLSKVYASAAELLPVLTDIDF
                     ELHTARSLAVIGESGSGKSTLLNLVGGLDYASSGSITIFGTSIETMNETELARFRNRH
                     VGFVFQYHHLLPDFSAIENVAMPALLAGRSFEEARETARQLLVDIGLGTRLEHRPGQL
                     SGGEQQRVAIVRALINKPGLVLMDEPTGNLDEGTGASVLDLVIALQKKNALTLVMVTH
                     SQKVAAACDECLLLAGGRARVQRRSGRRTGRA"
     gene            complement(13717..15024)
                     /locus_tag="PLD82_00235"
     CDS             complement(13717..15024)
                     /locus_tag="PLD82_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63846.1"
                     /translation="MRPELFLSLRYFLARKRRSSISLVSTISVLCVSLAVLVLIVVFS
                     VLNGFHEDMKSKILSKESHIILTAPGEGAIADYREAIKKVMRVPGVALALPQYEGQGL
                     IRWRNSVRGVKIRGVVPDLLKRDPDFMTRFAVPQGKFDLESRNNIVIGEVLSQLLGVR
                     VGDRVSLFVPPSQDADPLMIPLNIEYKIAGIFSSGFSEYDEFFVFMSLEDAQLLFGVG
                     DVAWAIGIKVDDFNRAREYREKINQATEYAYMSLTWMQLRRNLYQALVTEKTLIGVVL
                     FFVIAMAAFSIASTLIMVVMEKEREIGIVKSIGMTPDAVMRTFLIQGFMVGLAGTAIG
                     LLSGLLIASSMDSILRGIEGVVNAFSSSFHTLFSGLANLPEPRPWKLFPEDVYYVSTF
                     PVRIDAMEVFAICLGSVLLTTLCSLIPARQAARLKVTVVLHKE"
     gene            15332..16351
                     /locus_tag="PLD82_00240"
     CDS             15332..16351
                     /locus_tag="PLD82_00240"
                     /inference="COORDINATES: protein motif:HMM:NF027565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4899 domain-containing protein"
                     /protein_id="HOG63847.1"
                     /translation="MATANQPQQSLYTIIKGRFKAIDTNTFGLFLIFMNNNLRVSESV
                     TIIASHFSSMYETDPKQNWADVEERIMNLKWKGEFATSISRDTQTYVENALKGIRSEE
                     LYSLIKSNAVSKIENLLSMILVKPLGDKNIHMELSWDNASAEQIQTAKQERELRDTSD
                     GSQVDDSFSPQQEPDDKINVDDGSVILEVDFVLAPVSGIPIFELQPGDRIMVKINPAT
                     KRGMYFIELLNAKHDEEIVPVPATVTEIKLNKSNEYIVIVKIGEGIFGKVVEAEQVKI
                     KRFDPNTDKKTAANAQRNLAAAGFTKAEPVTKQDKAIRNLYWLMYLGGFVVLAVIMVI
                     VAAII"
     assembly_gap    16387..16486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16682..17629)
                     /locus_tag="PLD82_00245"
     CDS             complement(16682..17629)
                     /locus_tag="PLD82_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63848.1"
                     /translation="MSTDRAGIAILEPDPLYRGVFERIFFSSGYEVRYLETGGGLPVV
                     EPHDAVLLMDMVQGEKTAFDLLSGIRAEHPLIPVVVFCDEDIGGYLDRLAEYERVNVL
                     ARPFTREELLFFMDKLLYQDHAFGLEHYLKAGAVVQRFILKSTTEVRERIEETLGLAS
                     EWGFDFDYDFKIDLVLHELFLNAIYHAHGYEDEKKQGVPVTLPDAVRVEVEVGHDENR
                     FGVTITDFCGNLTRSRILESLRTLEQQKSVERLLAEGRSISDIFRQHGRGIDIVRKNS
                     GEYYFVIERGRRTQVVIIFDKHFEKDDQNASIKVLEVGE"
     gene            complement(17626..18984)
                     /locus_tag="PLD82_00250"
     CDS             complement(17626..18984)
                     /locus_tag="PLD82_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG63849.1"
                     /translation="MLTITFLAVFVGGLAIIVTTVIFKSIRTARKKRDASDHIDRGKN
                     MDAIRHLVEVVQANPDDLVSLFDLATLQIQTGLPFEGLQNVKKLLERNLAGSGVIHVE
                     AILLGAKAEFALGNRKEGYKYLMMAKGIDSTSLEVNVSLAAYNYDEGNYPDAYGYASR
                     VLKLEPDNVSANFYSGMAAFQQGNFDTAVGALEKVAALQPTNHQALYSLGRIYGKLGN
                     NAKASGAFGAALRHAPNDESRAQIYLQWGINLNKSMNHDKAVECFQHAVRLASSTEVK
                     KDALREIISIQEAARKVDKVLEALKAYLVLDPLDEIFKAKARYYGELHTNDKLKKYEM
                     ATLPDFVNYCCDIAKQIVRIDTIVATEINKDGSVDVLANRSTKTQNSNFQFRFVRTKG
                     EIGEFPLRDLYAKMRGNSGEKAFMVANTSFNESAVKFAQTRVVTLVNRETLLKILDKV
                     SG"
     gene            19192..20202
                     /locus_tag="PLD82_00255"
     CDS             19192..20202
                     /locus_tag="PLD82_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG63850.1"
                     /translation="MTRTGICCSLVAILSLASCSTTTQDVAQPQHLVVTSIVGTVKAW
                     KTEDKKKDVRSGFKLEEHWTVQTSGEDALCQMQTPSGSVIKISGNTTLRLSELYKDNK
                     LAVEKTGLELVAGKILVKARTLSGDDSFSVRTKTAVAGVRGTRFIVAYDDEKGTQVAV
                     ESGKVAVGQPVAVVLPAGMTNASREIMTALERSTEVVVAPNETIKVTLADNQATQKAV
                     ETVVTETLTSGTAVKPEEIRAAVVQIQAKLETAPITRPAKRTLTDADVEIKNDFVAVQ
                     ALTVTELAPPTGTPATNASGKTDGSTNKPATNATQKAPVVTQQPQIIKRDKEDVNPEE
                     RF"
     gene            20207..>21063
                     /locus_tag="PLD82_00260"
     CDS             20207..>21063
                     /locus_tag="PLD82_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOG63851.1"
                     /translation="MKRHGFIVQALILSVLALSLQGDPVSRTFISHEKGVVALDWSPD
                     GEYLATASLDGAVSLWNAEGLLVRKMQIHTKPALGIAFSPDGEVLATASADATVTVFT
                     PSGRIMHRMKSHRTNVLTVAWSPDGRFLASGSEDMTVRIHTKNGSLVSTLTGQMQPVT
                     AVEFSRDGKRLAIAERGVHLLIRETRLWGMEKLVKAHNDVVWDTAVSRDSQFFATCSK
                     DRSLKIWRADGSPHLALVNLPAEVWTIAFSPDGTKIACGLKSGTVLVYALPSGTILHT
                     FSGHKDGVLG"
BASE COUNT         4854 a         6356 c         5446 g         4307 t
ORIGIN      
        1 tcacaggtcc tctcgctcct gacctccgaa gtcaggcagg ccctgctcgc ctttgtcaac
       61 aagcatggca agatccaggt ccatgacagg gcagttctct gggtccatca cgggatggat
      121 acctcggcag taccgggcgt ggtcgcctca atgaaagaac tggcccgggc cttgggtcca
      181 tactgaggta ttcaggaggt tccggcgcag cccaatcgca aaagcgctcc accggaggcc
      241 ccatacccgt acgctggtcc ttgtcgatgc catggtgccg tttccaaaaa cgcgtttcgc
      301 ctcacaagga acccgtgtac ggagcaggca ttgcacgaag ataccgggga acctcctttg
      361 ggcccgccca tgtcaagccg gtctcactct ccggcgggaa tcaccggggg agcaagccgt
      421 tccacctcga gaacctcggc caggacggcc ggatcggtcc ggaaatacgc gcctcgtccc
      481 tgatagaatc cgtgtgcttc gatcagatgg atcgtgagat ccgtatacat gacctgcttg
      541 ttcagtctcg tgttttccac aattacggta ttcttcgcaa aaccgccgcc ctggtgccca
      601 aacgggcagg agattttacc gcgcacactg tccgacctga cctcgaaatg aggcggaacc
      661 atgacgaccc cgccaaaccc ttcctcggca gcatcacgaa aaaaccgcat ccggtcgaca
      721 atggcggcgt gggtcagatc caatcgcttg actgacagat catcctccac gatgatttcc
      781 cccagatgtc gggtatctgt tccaagaaac ccgtcgcggc tggtctttcc cggcccaaga
      841 cgtttttcga tggcatcctg ttcgggactc tgcctcatct cagccctcct tggtgttttt
      901 tggggaacag cctcaggccc gccatggccg actggcaagc gcaatgccgg caagccatca
      961 gccaaagctg tcgtaataga tcctgtccac aggaatgccg cattcgttga ggacagaaat
     1021 gcaggccttg atcatccccg gactgccgca gaggtaggcc tccacctcgc tccccgttgt
     1081 cccgagatgt ttgcgcacaa catccgtaat cagaccgctt tccccctgcc aagcgtcctc
     1141 cggcttgggg gccgagagcg ccggtatgaa ggtgaatgcg ggcaggctcg cctccagacg
     1201 ccgcatctca tccaggacaa agagatcccg gcgggaaaca gccccgaaaa aaaagcggca
     1261 acgccgggca gcgatctccg gttgggcgtg tgcccggtgt tcgatgtcgg ccaggatgga
     1321 gcggatgggc gccatgcccg agccaccggc gatacagatg atctcgcggg aggtttcctg
     1381 cagaaaaaat tccccaaagg ggccggtcaa cgagacacga tccccctctt tgagataccg
     1441 gtggacccag gtcgtgcaaa tcccgttcgg gacaaggcgg atcatgaatt caaccagacc
     1501 ggtctcgtgt ggcgccgatg acatggaata ggcccgggag acctgttcac tggaaagttc
     1561 gtattcggga acggcgagtt gcatgaattg cccggccttg tagctgatgt cggccggttc
     1621 ggtcaggcgg attcgcaccc gcttgatatc atgggtcaga tcctcgatgt actcgacctg
     1681 gccggagaac tccttgatgg aaaacagccg ggaggggact tcaatcgaca tgtcccgctt
     1741 gaccttgatc tggcaggaaa gacgaatccc cttttcccgg gactcgggct tgatccacgc
     1801 gagttcggtc ggcaggtagt cgccaccccc ggatgtcacc ttgagggtac acaacccgca
     1861 cgacccgcgc ccaccgcatg cggagggaat gaagatcccc tcctccttga gggttgtcag
     1921 gagcgattgg ccaccttcga catcgagaag cttttctcca ttattgatgt cgatcttcat
     1981 ccgaccgtag tggccaatgg tcatctcggc gagtacgaca aggaaggcca agccagtggc
     2041 gatggcggtg atcactccga cactgacaaa aaatgtttcc aaaggcgaca tgccggctcc
     2101 tttcgagcgt cagttgaccc ggatcatccc ggaaaagccg ataaaagcca gagccatgat
     2161 cccggtaatc acgagagaga tccctgctcc cgaaagtccc cggggaactc ttgatctcgc
     2221 cattttttga cggatcccgg ccatggcgac aatcgccatg gcccaaccca ggccgccacc
     2281 ggcaccatag gcagccgcct ggggcagcgt gtactcccgg atggcgataa agagggtcgc
     2341 ccccaaaatc gcacaattga ctgtgatcaa gggcaaaaag atcccgagcg cggcgtagag
     2401 tgaatccgag acgcgttcga tgatcatttc gacaagctgg acaaaggccg cgatcacgac
     2461 gatgtagacg atgtagcgca tatagaccag atcaaggggc acgaggatgt actgctcgac
     2521 cagccagttc aagacgctgg tgacagccat cacaaaggtg acggcgatcc ccaggccggc
     2581 cgatgccttg acctcggaag agacggccaa aaaggaacac atcccgaggt agttggtcag
     2641 aaggatgtta ttggtaaaaa ccgctgcaag gaaaatggag aggacactga tttcattcat
     2701 ggtttaccgt actccttctt tggaacggag catccgtccc cggacaatcc agatcaggat
     2761 ggccaggaca aagaacccgc caggtgccat caccatgatg ttccagttgg tccaccactc
     2821 gccgagaacc cgtaccccga agatcgagcc tgacccaagg agttcccgga caaaggcgac
     2881 ggccaggaga acccaggtgt agcccaagcc gttggcaatc ccgtctgcca tggagatcag
     2941 cggtttgttg gccagggcaa aggcctccgc ccggcccatc acgatgcagt tggtgatgat
     3001 cagcccgacg taggcaccga gggccttgga gatttccggg agcgttgcct tgatgatgat
     3061 gtcaaacacg atcacccagc tcgcgatgac cagggtttcc acaatcatcc ggattcttcc
     3121 cgggatcagg gagcgcagga gcgagactga aaaattggac aggccagttg taaaaaccag
     3181 cccgatacac atcaccagcg tgttttcgaa aacattggtc acggccagtg tcgagcagat
     3241 tccgaggacc tgcacgaaga ccggattgtt ggcaaagatt ccgtcaaaaa tgacccggcc
     3301 catccgtggt ttggcgagtg tcatggtccg gcctcctctt tccgtggaat accgccgggg
     3361 ttcttgttgg ttgcggaatg agcgtcatac aaaaagacgg cgttggacag gtcagcatcc
     3421 gtgcgcgcaa tggccgcaat atcccccgga gcattggaaa gaaccgtgtt gagcatggaa
     3481 cggacaccgt cgatcgttgc ggttgccccc gtgatcccgt caaattcggt cgccagagat
     3541 gaggccgttt tttcgggacg cgccgtcagg ggaccggtct tccccgagaa ctgtttgcca
     3601 aaccaaggct catcgattct cgcgccaagg cccggggttt cactgtgctc ggtgatcgca
     3661 agactcatca ggtgtttccg gtcggcacgg acaccgaccc aagcccggat cccgccccag
     3721 agtccctggc cccggccctc gaagacaaac ccgaggagct gccgccgacc gctggacggg
     3781 tcactcgatg cgccctgcct gatgctgtag atcgccctcc ctccggcatt ggtataggcc
     3841 ttgacctggt tcgaaaaaat ggcatccagt gttcgttgat catcgggtac gggcatccgg
     3901 gctgcggcca ggagggcccg ttttgaagcc agcgtctggt tgaccctgat ccggtcacga
     3961 ctggcaagat agacaccggt caggatcgag atacagaaaa ccgaaatggc aaacatgaaa
     4021 aagaccgtga aaatccgttc gcgtcgtttg ctggagaatt tcatggggtt gctcccggtg
     4081 caggtcgggc cttgcggttt ttaatcaccc agtcaatgat cggagcaaac atgtttccga
     4141 gcaggatggc aaacatcatg ccttcaggcc agacggaaaa aaccctgatc agcacggtac
     4201 tagcgccaat gatgacacca tagatccagc ggccgccatt ggtgactgcc gccgaaacgg
     4261 gatccgtcgc aataaagacg atgccgaaca ggaccccgcc cgccgtcaga ccgacaagcg
     4321 gatcgggaag cagaatcccg gaccgggtcg tcacttcggc cgcccagagc gggatccatg
     4381 tcgccatgaa tccgacaaga cagggtacga tgatcctcca gttggcggtc ttggtccaga
     4441 tcaggtagat ggcagccaga ccgatcagga tcgcactcga ttcaccgagg gaaccgggca
     4501 ctgttccgac aagaagatcg attcgggatg gtgcggccga ggccagtggc gtcgccgagg
     4561 taatggcgtc cacaccgggg gaccaggcac caaacccggc agggaagagc ccatgagtac
     4621 cggccgcagc cgatgcggcc tggggaaagt tccccaaaaa accggtcaag ggaagagccc
     4681 atacccctgt catggcctgg ccaaagctga catacaaaaa agcccggcca accatggcag
     4741 gattgaacac gttccgtcca aaccccccaa agaccatttt cccgaagagg actgcgacga
     4801 tggatccgag cgcgaccatc cagagcggga tcgtgggcgg gagggagagc gcaaagatgg
     4861 ttgtcgtgac aaaaaccgcc tgcgagaccg gctcgtggta cggacgaagc atcagtctct
     4921 cgacaatcca gccgacagtg gcagcgatac agaccatggc cagacttttc caaccataga
     4981 gccaaatggc tgcaaggacg aggggagaaa gtgccagaag catgcgccgc atgggcgcct
     5041 gtttcatgat ccagggcagg ggtttgacag tatccatgca gtcattctac ctgatgaacc
     5101 gcgcagatgt caaaggtcgc gatatcgccg tccgggggaa ctgtccgcat ccgacatacc
     5161 cactggactt tcgcatccct caaggatcct catcccggaa tctcttacat ccttcagtgc
     5221 agactgtcga ctgacatccc gggcctctcc ctcggcatca agcgccaaga cttttgccag
     5281 gctgtttttg gcctggttga tccggcgcag acaggttcgt ctcgacactc cgaacatccc
     5341 tgccagttca tcgaccctga tgtgatcgcg atagcgggca agcaggatct ctcgctccat
     5401 tgtgctgaca catgcaagaa tccccaatcc ggattcccat cccgggagag acgcatccgg
     5461 atactcccgc ccggtatcac acaggaccgg atcggcatcc gggatttcaa agtacctctg
     5521 tctccccatg cgccgcgcac aacgcctggc tgcgcgaagg acagcttttt cgagataagg
     5581 gaccagcgaa cccgagcagt ccagccggtg gatggatctg gtaacggaga ggaagcactc
     5641 ctggacaaca tcctcagccg aaaaagcatc caacccgcac ccatgcgcca accagatcag
     5701 ccggcgtctg gcaccacgac aggccctctc gagcaccaac aaccggtctg catattcttt
     5761 gttccgatgt tcaaacggtt ccatacttga atatttcgac tggaaacagg aattttcaac
     5821 tgcccgttat tttatattct atgtacaatt attccaatat ttttcttttg gaaaacatct
     5881 cgtgcagaat gatcccggcc gaaaccgaaa cattcagcga gtcaagccgg ggaagcatgg
     5941 ggatagtcag tttccagtca cagcgctcga ggagaagccg gcggatcccc gctccctccg
     6001 aacccagcac gacgacaaag ggagaatcaa aggcaaactc gcggacaggt ttgccgtcgc
     6061 tcccgacagc cgcagcgctc cagaacccca tctccttgag ccgttcgaga acccgcacga
     6121 gattggtttc ggtaatgacc ggaacatggc aatcgccacc gcacgcagcc ttccgtgcga
     6181 caggtgtcat cccgctggca ccatccttgg ggataatgac gcaggaaacc ccgaactggg
     6241 cggcgctccg gacaaccgcg ccaagatttc ccggatcctg gacatgatcc agaaccagga
     6301 cccgtggcca ctccccccgt tcaactgctg ccgcaagctc acgttcccag tcccagtgga
     6361 caatatcctc gggaaggggc tccagccgta tttcttcttc ctccgtctcc ctgtcctctc
     6421 tcgcctctac agcaggacgt ggagctttgg gtggccgtgc ctgcgcgcgc ccatccggta
     6481 cgcgccccgg agccttcccc ttctggccaa gttcgaggac aaagccctgg tggacaagtc
     6541 ccggataaag ctggtccagt tccctcctcg gccccgacag acagacgata ccgagtttga
     6601 cggcaagatc ctgcatcaac ttgccggcac caccttcatt tccacgggcc aagagaagcc
     6661 gcccgccaac cggccgacga caggttttca gatattcaag aacggcgttc tttccgtaaa
     6721 taagcgtttt tttcatgtat gcttctccca gacagttccc tggggcgtgt ccttgaggat
     6781 gatccccttt ttggccagct catcgcggat gcgatcactc tcggcaaaat tcttcgcctt
     6841 gcgggctgca ttccgctcgg caatcattgc ttctatttcg tcatccagac tttctccgga
     6901 accgaagtcc aggacattca gaacctcgtc cagacgcttg aaactccgca ggacggcatc
     6961 ggcgctctcc ttccccagag ggaagtgttc ccgaatcttc gggaggacgg gaaacacggc
     7021 tgccatggct tcgctgatgt tgaggtcgtc ctcaatcgcg gccgtaaaac cgccaagcat
     7081 ggactcgagg atcgcgccca ccgccgcagc ctgaccggac gggatattgt ttccatccgc
     7141 atccggaccg gaaaaacggg cgacatcggc caaaaacgta tccagtctgg caagggccgc
     7201 cttggatgct tcaagcccct gttcggaaaa attcagtgtc ttgcggtaat gagtggaaat
     7261 cagaagatag cggatgacct tgggtgacca gcctttggag acgagatccc ggaaggtaaa
     7321 aaaattcccc ttcgatttgg acattttctc accgtcaacc aggagccagg cacagtggag
     7381 ccatgtccgg acaaagggct tcccgcttgc ggcctcgctc tgcgcgatct cgttttcgtg
     7441 atggggaaac atgttgtcaa tcccgccggt gtggatgtcc attgtctcgc caaggtattt
     7501 catgctcatc gccgagcact caatatgcca gccgggacga cctctcccga agggcgagtc
     7561 ccagacaaca tcgccatcct tgtcactgta ccccttccag agaacgaaat ccgaaacatg
     7621 atccttttcg tattcgtcac tgtcaacccg cccggaggcc ccggcaacca gcccctccct
     7681 gtccaggtgg gccagttttc catactcacg gtagcgctcg atactgaaat acacggagcc
     7741 ttctttttgg taggcaaatc ccttgtccag gagattccgg atcagggaga tcatctcggg
     7801 aacatgtttg gtcgcctctg ggtaaaccgc agccggggtg atccccagag ccttgatgtc
     7861 ctcgaaaaaa gcctgcttgt agacaccggt gtactcgtcc agcgccatcc ccttggcaat
     7921 ggagttcctg atcgtcttgt catccacatc cgtcagattc atcacctggg tgacatcata
     7981 cccggcaaac cgcagaacac gatggagcag atcttcgaag atataggctc tgaaattgcc
     8041 gatgtgcgcg taatcgtaga cagttggccc gcatgtgtac attgtcacct tgccggggac
     8101 gaggggcgaa aattcttcga ccctgcgggt cattgtgttg tacaactgta cagccatgat
     8161 cgtcctcctc tgacgcacaa acgcagcctg aaaccggcat cctctaaagg tacaaaaaag
     8221 tgcgccaccg tataccatac ggcggcgcac caaagggatt ggatcggaga attatcaaaa
     8281 acccgcactc acctatatag caagcgccgt gccaatcaaa ataataatcg aaaaattcct
     8341 attttattgt aaaactataa gttataacac gcttagaaag cttaacatta tgattttttg
     8401 ttatatggcc aaattatgta cagttttttt gatcaaattc tggccacttg gctaattatt
     8461 ggccaatgcc gagccgcttc attttggagc gcagcgaact gcgccccaac tgcaagacct
     8521 cggccgcccg actgacattc ccgttacaat gctccaaggc accctcgatc actctttttt
     8581 cgatggcatc cagcgagatc ggcgattcgt agcctatcac aagatgcaag ccaccatcat
     8641 ccagcgttct gcatttctcc ggcatgtcca tcaacccgtg ttcaagctgc acaaaatcca
     8701 gatcggcccc ttttgcaaga atcgtttccc gctctaccac atttttcagt tcacggatat
     8761 tccccggcca aggataccgc tcaagacgtt tctttcccgc ctcggtaatc cggtactccc
     8821 ggtgggagtg cgcctgcttc ccgataaaat gccgcgtcag gagcacgata tcctcaacac
     8881 gttcacgcaa tggcggaaga tggatattga ggacattgat ccggaaatag agatccttgc
     8941 ggaaacggtg ctggtccacc tctgcctgca aatccttgtt tgtcgcgcag acgatccgca
     9001 catctacatc cacttcgccg ccaccgaccc tgtgcacgcg cctcgtctcg agaaaccgga
     9061 ggaggctggt ctggagccgg acttccatgt ccccgatctc gtccaaaaac actgtccctc
     9121 cgttggcccg ctcaaaaaca cccttttgtt gccgctcagc cccggtaaaa gcgcccttct
     9181 cgtgaccaaa aaactcgctc tcgatcaggg actcggggat gctggagcaa ttgatcggga
     9241 taaacgcttc ttgcgaacga cggctgtgca tatggacatg tcttgccacc agttccttgc
     9301 ccgtcccggt ctctcccgtc acaagcacgg tcacatccgg gttggccgcc gcgcgttcga
     9361 tcagggcacg gacatcccgg atactttgtg attctccgat gatgggcaga tttcccgaat
     9421 cacggtacat cgtgagttca ctctgctgcc tgcgggtttt gagggatttt tcgatcgcaa
     9481 agaccagttc gtcattgtga accggtttga gaaaatactc ttcggccccc agccggatgc
     9541 actcgatagc cgtacgcaca tcctccatcc cggtcatcat gatgatggcg gtttcgggcc
     9601 agcgcttgcg gatatatccg agcatttcaa ccccggaacc atcctgcaaa aagacatcca
     9661 gcaggatgat atcgaaactc tccgcctcca atgccttcct ggccgcaact gtgtcgtaga
     9721 cagccgtcac gctgtaattc cgcaatctga gcatctgcgc catcttgtcg cagaactcgg
     9781 cctcgtcatc cagaatcaga atctttgaca tccctcagcg tttccttcca tccgcatttc
     9841 cctgatgagc ctccaacccc gaccctgcct tcaacaaacg aagcaaggat gtataatagc
     9901 gcggctcgat atcggtctcc gccacaccga gtgattgcaa caggctcctg acctgcgcct
     9961 ccgcggcagg aatctccccc tgtccgtcca gcaaagcttc gatctcgaca aaccagccca
    10021 ggccgctcac ctcggccaat tcaatgtgca cacctggctc gacctcccag acatgtccgc
    10081 gcttgcgctt ttcaatcgcc acgcggtatc cgagggactg cgcaaaagcg tgaaaggcat
    10141 cggccgaatc gaccgtaaac tcgacttcgt tgttcacctc gacgccgtca cgcatccctt
    10201 tttgcttgat cgtcacgaca tccggaccgg gcagcccgtc cccggccccg tcatttctca
    10261 accggaaagc ggtacttccg ggattggcca cggattcgaa gtagacatcc cgcttgtcaa
    10321 acggaacgcc ccgcccgtac cgcgcaccga gggctgtttc aatcccttga gcatccttca
    10381 catgagcttt gatctcgact tcccaggcca tgttcatgtt cctcctgcca aagaccgcga
    10441 aagcaacaca agaaccgagt ttgcccggac cctgagtatc ccgccggcac ataccggctc
    10501 gcccccgtct ggatggacca gcccctgcgc ggtatccacc ttgatccacc agccgccatc
    10561 gggactcgtc ggcaagacgc acctctgtgg ccgtccggtc gcattgcaga gcatgtagat
    10621 cgccggctcc attcccttcc cggccagcat gcaccccagg agggggatcc cgctttcgaa
    10681 ctccggtttc cccggttccc tgccatgcca cgagatatcg gggatcgggc tgccctcttc
    10741 gggttgaccc gaaaagaagg tctcgcgccg caacgaccgg aatctcttcc gcaagcggat
    10801 catgtcccgg acaaacctga cctgctccgt ttcacgatca gccagactcc agtcgaccca
    10861 ggagatctcg ttgtcctggc aataggcatt gttgttcccg ccctgggttc gcaaaaactc
    10921 gtcaccggcg aggagcatcg gggttccctg ggagagcatc atgatggcaa ccgcatttct
    10981 tgcctgtctc cgcctgagcc gcaagacacc cgggcggtcg gtcgcgccct ccacaccgca
    11041 attgaagccg gtcatcctcg ccatttccaa gattgtgctt ccgttcatgg ctaaagagat
    11101 cgttgagggt aaatccgtca tggcaggtga tgaaattgat ggagtgttgt ggcgagcgcc
    11161 cgtcatccgc atagaggtcg gaacttcccg caatccgggt ggccagtgcg ctgctcgacc
    11221 cggcatctcc acgcaagaac cggcgcagat cgtcccgata ccggccattc cattcggccc
    11281 agcgccccca ggccggaaaa ctcccgacct gatacaaacc cgccgcatcc caggcctccg
    11341 cgatgatctt tgtcgaggcc aggccagggt cctgggcgat ctcttcgagg accggcgggt
    11401 tgaccagaac attcccctgg ctgtcacgac caagaaccga cgcaagatca aaacggaatc
    11461 cgtccacatg catctcggaa acccaatagt ggagacagtc catgatcaac cggcgcacac
    11521 gggggtgatt gcagttcatc gtgtttccac accccgaata attcctgtag ccaccctgtt
    11581 catcaagcat gtagtagata ttgttgtcca gccccttgaa cgagatcacc ggcccgcctg
    11641 ttccgccttc gccggtgtgg ttgaacacaa cgtccaggat gacttcgatc cctgcacgat
    11701 ggagtgcacg aaccatctcg cggaattcat ggatctgccc gccctgcagg gcactggctg
    11761 cataagaggc cttgggtgca aaaaacgcac aggtgttata cccccaaaaa ttcaccagcc
    11821 gctctccggt ctcggggttg agacggactc cggcctcttc atcgaactcg ttgaccggca
    11881 tcagctcgac tgctgtcaca cccaggctga caagccaggg gattttttcg gtcaaaccgc
    11941 gataggttcc cggtgcgaca acagcagcac tatgatgaac agtgtagcca cggacatgga
    12001 gttcgtagat gaccgtttcg gagagcggtc gattgagcgg tcggtcaccc tgccagtcaa
    12061 attccttttc cggaagaaaa cagcgccgga ggtacgcacc atggtgcgtg gcactccctt
    12121 cgcggaccag aggccggccc cagatcgctc cgcccgtcaa tgccatcgcc cagggatcgg
    12181 ccaggacgac actcccgtca aacagcaatc cgtttgcagg atcccggggg ccatccgcac
    12241 gatacccgta ccggatattc tcgggcagac cgtgaaccag ggcatgccag acatctccgg
    12301 tccggttttg aagcggatca agggcaagct cgaaccaggg ttcctgcacc cccgtcgtaa
    12361 aaaatacgat gctcatcgac cgggcaccgg cagagacgac cgaaaaattg atcccggcct
    12421 cggttctgga cgctccaaag ggaaatggat gcccgccata cacccgcagg gatactcccg
    12481 aaacctcaca ggtacggatc atcgttcctc cagtcacgtc ccgtcgaatc acggaccgga
    12541 ctcagtgtac cccgaatatc ttccgtatgg aagagatgag tacacgatcg gactgatcct
    12601 ccccgctcca tacacccaaa acgagcgtca gattttcccc gtcgacccat ggcttggaga
    12661 gtgcgtacgt cccgctcgca cagcccggat cgctcaacca gagctgctgc ccatcacgca
    12721 agctgccgtg catctgcaga atcagtcctg ccaccggact ggaaccggtc ttgcgcttct
    12781 tccggcatga aaagaggaat gtccggtcca gctgcttgcg gacccgtttt tcgtcatcac
    12841 tccccaggcg taccgaaact gtctgacggg catcatccca tgaccagcga gccgtcggaa
    12901 tgacatcgcg gacccccgtc aggagaatct caatctcaaa acgctccgag gcccgcatcc
    12961 tcaggccctg cctgtccgtc gaccgctcct ccgctggacc cgggcacgcc cgccggccag
    13021 cagcaggcat tcatcacagg ccgccgctac tttttggctg tgcgtaacca tgacaagcgt
    13081 cagggcattt ttcttctgca gagcgatcac gagatccagc acactggcgc ccgttccctc
    13141 gtcaaggttt cccgtcggct cgtccatcaa gaccaggccc ggcttgttga tcaacgcgcg
    13201 gacgatggca acccgctgct gttctccgcc ggagagttgg ccggggcggt gctcaagcct
    13261 ggtccccaag ccgatgtcaa ccagaagctg cctggctgtc tcccgggcct cctcaaaact
    13321 cctgccggcc aacagggcag gcatggccac attttctatc gctgaaaaat ccgggagaag
    13381 atggtgatac tggaagacaa atccgacatg acggttcctg aacctggcca attcggtttc
    13441 gttcatggtc tcgatcgatg tcccgaaaat cgtgatgctt cccgatgaag cgtaatcaag
    13501 accgccgaca aggttgagca gtgtgctctt cccgctgccg ctctctccga tgacagccag
    13561 ggaacgcgcg gtatgcaatt caaaatcgat atcggtcaaa acaggaagga gttcggcggc
    13621 gctggcatag actttcgaga gcccctgcac ccggacggcc acttccgccg tctttttgac
    13681 tcccctggat ggcgttattt tcactgtgct cattgtctat tccttgtgca gcactacggt
    13741 gaccttgagc cgggccgcct gccgcgccgg aatcaacgag cagagggtgg tcaacaggac
    13801 ggaaccgagg cagatggcaa aaacttccat ggcgtcaatc cggaccggaa acgtgctgac
    13861 gtagtacacg tcttcaggga agagcttcca tggccgcggc tcgggcagat tggccaatcc
    13921 cgaaaagagg gtgtggaaac tcgaactgaa ggcgttgacc acgccttcaa tcccgcggag
    13981 aatgctgtcc atggaggatg caatcagcag tccggagagg agaccgatcg ctgtcccggc
    14041 aagccccacc atgaaaccct ggatcagaaa cgtgcgcatg accgcatccg gagtcatccc
    14101 gatcgatttg acaatcccga tctcgcgctc tttttccatg acaaccatga tcagggtact
    14161 ggcgatggaa aaggccgcca tggcaatcac gaaaaagaga acaaccccga tcagggtttt
    14221 ctccgtcaca agagcctgat agagattgcg gcggagctgc atccaggtca gtgacatata
    14281 cgcgtattcg gtcgcctggt tgattttctc ccgatactca cgggccctgt tgaaatcatc
    14341 gaccttgatc ccgatcgccc aggcgacatc cccgacgcca aagaggagct gggcatcctc
    14401 cagtgacatg aaaacaaaaa actcgtcata ctccgaaaac cccgaggaaa atatacccgc
    14461 aatcttgtac tcgatattca ggggaatcat cagaggatcg gcatcctggc tgggcgggac
    14521 aaaaagcgaa acccggtcac ccacccggac accgagcaat tgggacaaaa cctctccaat
    14581 cacgatgttg tttctgctct caagatcgaa tttgccctgg ggaaccgcaa atctggtcat
    14641 aaaatccgga tcccgcttga gaagatccgg aacaaccccg cgaatcttga ccccgcgcac
    14701 actgtttctc cagcgaatca gaccctgccc ctcatattgc ggcaacgcca gtgccacccc
    14761 gggaacgcgc atcacctttt tgatggcttc cctgtaatcc gcaattgccc cttcccctgg
    14821 ggcggtcagg atgatatggg attccttcga gagaatcttg ctcttcatat cttcgtggaa
    14881 accgttcagc acggaaaaaa ccacgatcaa cacaaggaca gccagcgaga cacagagaac
    14941 ggaaattgtc gacaccagag aaattgagga ccgccgcttc ctggcgagaa aataccgcag
    15001 actcaagaac aattcagggc gcacagaaca attccttcgg gcagaccggt tgcacttccc
    15061 cgttaagacc gggatacttc tcaaccgata cttgatatga gttctttcag tctaccaagg
    15121 caggtgtatc cctgtcaatg gggctgaagg ccgctcaaga gaaagatttg actttccagc
    15181 ccggcgctgg cactatactg tacagagtga aataccgatt ttggacgcac cgtactctgt
    15241 acctcgggtt tcccgcccgc cttcagacag ccgtagtaca gaccagacgg gaaatccggt
    15301 gctcccggtg gaagaacaca ggaaacgaga tatggcgacc gctaaccagc cacagcagag
    15361 tctgtacaca attatcaaag gccgtttcaa agccatcgat acaaacacgt ttggcctctt
    15421 cctcatcttc atgaacaaca atctcagggt ctccgagagt gtgacgatca tcgcctcgca
    15481 cttcagctcg atgtacgaga ccgacccaaa gcagaactgg gccgatgttg aagaacggat
    15541 catgaatctc aagtggaagg gtgagtttgc cacaagcatc tcgcgggaca cccagacgta
    15601 cgtggagaat gccctcaaag ggatccggtc cgaagaactc tacagtttga tcaagtcgaa
    15661 cgccgtcagt aaaatcgaaa atcttctttc gatgatactg gtcaagcctc tgggtgacaa
    15721 gaatatccat atggagctct cctgggacaa tgcctcggcc gaacaaatcc agaccgccaa
    15781 gcaggagcgg gaattgcggg atacttccga tggctcgcaa gtagacgact cgttctcacc
    15841 ccagcaggaa ccggatgata aaatcaatgt ggacgacggc tccgtgattc tcgaggtcga
    15901 ttttgtcctg gcacctgtct cggggatacc tatcttcgaa cttcagcccg gcgacagaat
    15961 catggtcaag atcaatccgg ccaccaagcg cggaatgtat ttcattgaac ttctcaatgc
    16021 caagcacgac gaagaaatcg tccctgttcc cgcaaccgtc accgaaatca agctcaacaa
    16081 atccaacgaa tacattgtca tcgtaaaaat cggcgaagga atatttggaa aagtcgtcga
    16141 ggctgaacag gtcaagatca aacgttttga tcccaacacc gacaaaaaaa ccgcagccaa
    16201 tgcccagcgt aatctggccg cggccggttt taccaaagct gaaccggtca ccaagcagga
    16261 caaggccatc cgcaacctct actggctgat gtatcttggc ggtttcgtcg ttcttgcggt
    16321 catcatggta atagtcgcag cgataatctg acgactacgg gtagtcgcag cgataatctg
    16381 acgactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacta cgggtagtcg
    16501 cagcgataat ctgacgacta cgggtagtcg cagcgataat ctgacgacta cgggtagtcg
    16561 cagcgataat cagggttttc cggcctgtca ggtatcacag aaccgtgcca tatccgggaa
    16621 accggacggt tttttcattc acagaccatg tcctggtcgt ttactgaatg gtacgagtcc
    16681 attactcgcc gacttccaga accttgatcg aggcattctg gtcatccttc tcgaaatgct
    16741 tgtcaaaaat aatgacgacc tgggtacgcc tgccccgctc gatcacgaag taatactccc
    16801 ccgaattctt gcggacgata tcgattcccc ggccatgttg cctgaagatg tcgctgatac
    16861 tccgcccttc agccaggagt ctctcgacac ttttttgctg ctccagtgtc cgcaatgact
    16921 caagtatcct tgaccgagtc aggtttccgc aaaaatcggt gatcgtgaca ccgaatctgt
    16981 tctcgtcgtg tccgacctcg acctcgaccc ggacagcatc cggcaaagtg accggaacgc
    17041 cctgcttttt ttcgtcctcg tatccatggg catgatagat cgcgttcagg aagagctcgt
    17101 gcagaaccag atcaatcttg aaatcgtaat caaaatcgaa accccattcc gacgccagcc
    17161 caagggtctc ttcgatccgc tcacggactt ccgtcgtcga cttgagtatg aatcgctgaa
    17221 caacggcccc ggctttgaga tagtgttcca gtccgaaggc gtggtcctgg taaagcagct
    17281 tgtccataaa aaacaggagc tcttccctgg tgaagggacg agccaggaca ttcacccgct
    17341 catattcggc cagtctgtca aggtatcccc cgatgtcctc atcacaaaaa accaccaccg
    17401 gtatcagagg atgttcggca cgaattccgg aaagaaggtc aaaggccgtt ttttctccct
    17461 gaaccatgtc catcaacagg acggcatcat ggggttcaac caccggcaag ccgccaccgg
    17521 tttcgagata gcgtacttcg tatccgctgg aaaagaaaat tctctcgaag acgccgcgat
    17581 agagcggatc aggctcgagg atggcgatgc ccgccctgtc cgtactcatc cactgacctt
    17641 gtccagtatc ttgaggagtg tctccctgtt caccaaggtc acaacccggg tctgggcaaa
    17701 cttgacggca ctttcattga acgacgtatt ggcaaccatg aaagccttct ccccgctgtt
    17761 gccacgcatt tttgcataca gatcccggag cgggaactca ccaatctcgc cctttgttct
    17821 gacaaacctg aattgaaaat tggagttttg cgtcttcgtg cttctgttgg cgagcacgtc
    17881 gacacttccg tccttgttga tctcggtcgc aacaatcgta tcgattcgaa caatctgttt
    17941 tgcaatgtca caacaataat tgacaaaatc cgggagagtc gccatttcgt atttcttgag
    18001 cttgtcgttg gtatgcaact cgccgtagta tcgcgccttg gctttgaaga tctcgtcgag
    18061 tggatcgaga acaagatagg ccttgagtgc ctccagaacc ttgtccacct tgcgggccgc
    18121 ttcctggatg gagatgatct cgcggagagc gtctttcttg acctctgtcg acgaagcgag
    18181 acggacagca tgttgaaaac actcgaccgc tttatcgtga ttcatcgact tgttgaggtt
    18241 gatcccccac tgcaaataga tctgagcccg gctctcgtca ttgggtgcat gcctcagtgc
    18301 ggcaccgaaa gccccgctgg ccttggcgtt attgcccagc ttcccgtaaa tccgtcccaa
    18361 cgaatacagt gcctgatggt tggttggctg cagagccgcg actttttcga gagcaccaac
    18421 cgctgtatca aaatttccct gctggaaagc ggccatcccg gaataaaagt tcgccgagac
    18481 gttgtccggt tccagcttga ggactctgga agcgtagcca taagcgtcag gataattccc
    18541 ctcgtcgtag ttgtatgctg ccaaagaaac gttcacctcg agactcgtgc tgtcgatccc
    18601 tttggccatc atcaggtact tgtacccttc tttgcggttc cccaaggcaa attcagcctt
    18661 ggcgccaagc agaatcgctt cgacatgaat cacccccgag ccggcgaggt tccgctcgag
    18721 gagctttttg acattttgca ggccttcaaa cggcaatccg gtctggatct gcagggtagc
    18781 aagatcgaac agggaaacca gatcatccgg attggcctgg acgacctcca caagatgcct
    18841 gatggcgtcc atgtttttcc cgcgatcaat atgatcactg gcatcccgtt ttttcctggc
    18901 ggtacggata ctcttgaaaa tgaccgtcgt cacaatgatg gccaacccgc caacgaagac
    18961 agccaggaac gtaatcgtca acatggacat ttctccgtca cgaaatcacc cctcatcgat
    19021 agactctata gtatacgact ctccccggga agacaagcgg tgatcatgcc tctatctgta
    19081 aattgtggtt gaataacccc ggttttgcag taatatgaaa ggaaacggga tttcgtcgtc
    19141 ctggtgcaga tgcagacccg tatccggcac gcaagactct ggaggtatga catgacccga
    19201 acaggtatct gctgttctct ggttgcaatc ctttccttgg catcgtgctc aaccacaacg
    19261 caagacgtgg cgcagcccca acatctcgtg gtcacctcga tcgtgggcac ggtcaaggcc
    19321 tggaaaaccg aagacaagaa aaaggatgtc cgttccggat tcaagcttga agaacactgg
    19381 acagtccaga cttccggcga ggatgcgctt tgccagatgc aaaccccgtc cggctccgtc
    19441 atcaagatct ccggaaatac cacactccgt ctctccgaac tctacaaaga caacaagctg
    19501 gccgtcgaga agacagggct cgagctcgtt gccggcaaga ttctcgtcaa agcccggact
    19561 ctttcgggag acgacagctt ctcggtccgc accaagacag ccgtggccgg agttcgaggg
    19621 acccgcttta ttgttgccta cgacgatgag aagggaaccc aggtcgctgt ggaatccgga
    19681 aaagtcgccg tcggacagcc cgtagctgtt gtgttgcccg cagggatgac aaatgcctcc
    19741 cgtgaaatca tgacggcact cgagcggtca acagaagtcg tcgtcgcacc caacgaaacg
    19801 atcaaggtca ccctggcaga caaccaggcg acacaaaaag cagtcgagac tgtcgtcacg
    19861 gagaccctca cctccggaac agcagtcaaa cccgaagaga tccgggcagc ggtggtccag
    19921 atccaggcaa aactggagac ggctccgatc acccgacccg caaagcgtac cctgaccgat
    19981 gccgatgtcg agatcaagaa cgacttcgtc gcggtacaag ccttgaccgt caccgagctc
    20041 gcacctccga cgggcacccc ggccacaaat gcctccggaa agaccgacgg ctcgacaaac
    20101 aagccggcca ccaatgccac ccaaaaagct cccgtcgtca cccagcaacc ccagatcatc
    20161 aagcgggaca aggaagatgt caatcccgag gaacggttct gaaagcatga aacggcatgg
    20221 cttcattgtt caggctctca ttctttccgt gcttgcactg tccctccagg gcgaccctgt
    20281 ttcgcggacc ttcatttccc atgaaaaggg tgtcgttgcc cttgattggt ctcctgacgg
    20341 agagtatctg gcaacggcct ccctcgatgg agcggtgagt ctctggaacg ctgagggtct
    20401 tttggtccgc aagatgcaga tccatacaaa gccggcactg ggaatcgcat tctcccctga
    20461 tggtgaagtc ctggccaccg catccgctga cgccaccgtc acggtattca caccctcggg
    20521 ccggatcatg caccggatga agagccaccg gacaaatgtc ctgaccgtgg cctggtctcc
    20581 ggatggccgg tttctggcga gtggttccga ggatatgacg gtgcgaatcc atacaaaaaa
    20641 cggctctctc gtctcgacgt tgaccggaca gatgcagcct gtcacggctg tcgaattttc
    20701 acgggacgga aaacgcctgg caatcgccga gcggggtgtg cacctcttga tccgtgaaac
    20761 caggctttgg ggaatggaaa aactcgtcaa ggcacacaac gacgttgtct gggacaccgc
    20821 tgtttcccgt gacagccaat tttttgccac ctgctcaaaa gaccgttccc tgaaaatctg
    20881 gcgcgccgat gggtctcccc atctcgccct cgtcaacctc ccggccgaag tctggacaat
    20941 cgccttttcc ccggatggaa cgaagatagc gtgcgggctg aaatccggga cggttctggt
    21001 ctatgccctc ccgtcgggaa ccattctgca cacgttttct ggtcacaagg acggcgtact
    21061 cgg
//
LOCUS       DAOSHE010000003        17573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_2634_length_17573_cov_4.615424, whole genome shotgun sequence.
ACCESSION   DAOSHE010000003 DAOSHE010000000
VERSION     DAOSHE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17573
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2634_length_17573_cov_4.615424"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..608)
                     /locus_tag="PLD82_00265"
     CDS             complement(60..608)
                     /locus_tag="PLD82_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG63852.1"
                     /translation="MILRISLILSAFLALSACSSPAQNKTTPTNEPIFRPWGIIGAKI
                     TDTMEGILVTDVIQGSTAESAGMRVDDYIRSVDGKRATSTEQLMSDIRSRLPGTVMQV
                     QTERNRRTTMLQVTVGMFPLDEQLWLMASSALKAWDSLRARQLSDEFARSCPPSSRYA
                     ARMARLRTELGQQEGTGNKPQP"
     gene            700..1056
                     /locus_tag="PLD82_00270"
     CDS             700..1056
                     /locus_tag="PLD82_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG63853.1"
                     /translation="MKKWGKFVEGLYLVAMAERLDDVARVALIREGNALFNEGRIEEA
                     KPKFIRAGYIDGIIRVADHYYYTLKKPAAALILYRHAGCTAKSEEIIETIVAVIRILL
                     AEDGADEGSGRGEAQG"
     gene            1037..2374
                     /locus_tag="PLD82_00275"
     CDS             1037..2374
                     /locus_tag="PLD82_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG63854.1"
                     /translation="MAKRRDEGLFGGDLGEQSAPLARRMRPRSFAEFAGQAHVLGPGK
                     PLRRFAEEGRHFSWIIFGPPGCGKSAFAWLVAASAQAHFESINAVTSNVTELKAAIEA
                     ASSRLRYEGRRTLLFIDEIHRFNRMQQDALLPAVEDGTVHLYGATTQNPAFCLNPALL
                     SRCQLVEFRALTSADLAPLVRRTLEDEERGLGSQELTIQDDALELLVAASSGDARRLL
                     NLLELAAASLEPQGTITVETVRDASAGQAAPVYDRDGDNHYDITSAFIKSMRGSDPDA
                     ALYWLARMLEGGENPDFIARRIMIAASEDVGLADSGALSVAVSAAQATAMLGMPEARI
                     VLAHAVLRIALAPKSNSACVGIDAALADLRANGPLPVPEHLRDAHYALAKKLGHGVEY
                     RYPHDAPGHWLVQEYLPEARRFYKNSGQGHEGPLQKWLDLLRSSVKPDNTEGT"
     gene            2620..4485
                     /locus_tag="PLD82_00280"
     CDS             2620..4485
                     /locus_tag="PLD82_00280"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOG63855.1"
                     /translation="MAYDKQAIDAARKSGMLESSMDASALSLDDQEKAALRRRLESAS
                     AGMDSGDDEDEESSGRGENIYERLRRRDAESAVPAGGESPAGLAGLLTKIIIFFKSLF
                     SSVSKEAMTKKKAFKDLERNLQEFPIPVYDPKMTAVRKTFIDYLALIQKKVESFSDFF
                     SANFLVSADDFVSDRSPCFVRFVIETQLSMEQARMLRELRTFDLQRSFSVRGEEITQR
                     DTELLIKKFVESFDGANLQRIEETLEFYVAVLNLKNYNFQGIFSLFQVDGAKDGVFRD
                     FSLEHCLGQLRNLDSYLAGINFNKLREDHYVWFDRFRESIEGGMEISVPRYLSEDFRT
                     MIGPLRKLCDKGAVTTLVRIGSEEIGYKPKPVVNTLSWVEKYRKLAESSFKDHLERNI
                     ILLREEQTSAQVGDLFEGYRPFTWLPLLNRDTNDRLKRAGGPQFASVFLFNLVWNFLD
                     RIYIERFKRSVNSVVVEGEFRKKEIAADFANTYYALDDFYERMKQMLERLTPESSIAI
                     RINGYVEGKMENPIAAKKFEADLEDLDAELSEMAEHAGIQLMKMHRHMDSILRDFKGL
                     RALDLINAKAIGGVSNRNLMFVYDKLVVSFKKLETILGHFIVVREQIPGSGVPQA"
     gene            4482..5525
                     /locus_tag="PLD82_00285"
     CDS             4482..5525
                     /locus_tag="PLD82_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG63856.1"
                     /translation="MSSSRLRLVFIGIVSAIFLAMLVAWGSAVVPFVIGIVIAYILDP
                     FVVFLQRRLHLPRTLAVLFVFLLFLVVFVLLIAFLYPMLANGVSSITRTVADNEAKIT
                     LFLKDAENWIEQLDLPFAIDSGKMITSMFAEAETFIKHFFGELSSFAISIVGSLPLLI
                     LIPLIIFYLLKDKEKIFDVLKKYTRDDNEARIRIVFDEVNTRLGGYVKGQFFLSWIVA
                     NITTVAMLLFVVPYAVLIGLANGVLNIIRYFGPVIGAVPAIILELVSFTSTGHFLGVV
                     FFFVIMNILVSTILSPKIFAHATNLHPLVVLLALFVGSTAMGMMGMIVAIPVAIILQT
                     LIRIVFDTYIKEI"
     gene            complement(5528..10573)
                     /locus_tag="PLD82_00290"
     CDS             complement(5528..10573)
                     /locus_tag="PLD82_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63857.1"
                     /translation="MLTDFIHILLGLAFCSIASSHGIWTRYPAFSWQPLFLSFVLHGL
                     ESLLSALTPALGDSTFFSVLRILISLAAWQSLLESARRTLAAQHSALPLPVYWVPAPL
                     ALSFGLEGLAALQIAITLIVAFPAAFLVARTLSKEARIARWGIGQSLKLLGILLPVLV
                     LTWLIPALSGYNLAPSGQADAVAVLVLVLRMGIALGIVLLIRRIDAKLSDQSEKQGPF
                     SRRLLLRASLLLGILAGSWCTAEILGSMEGERQLQTLRNHGQAIAAMLPSSYILQLSF
                     TGKDASTPVYNRLARHLELLIRQPPGYTRAILTTMIETTVIHGPGAAIDGEPVPASGS
                     TRTTIPRGFWQAWSSRTTTCATVDEGGHRHDIIWAPVINPRTQTVIAIIELSREYHLR
                     SSTVVTARLLPLVLGFAACTILLAGFVALGYRSRQTRFSTGARHTEIWLIAALGTVLT
                     IVTAILTQKAADRETRDMLYHLSAYRTRLIHDTILQRAHDLEALERFFSSSKTVSRQD
                     FSAFASPLITRSKSFRYLIWAPHVSREERASFEAEGRAAGLQDYSFKERNDRHEMFSA
                     GLRREYWPAHYVSPGDDTLHLHGLDLGTDILADEAMQISAATSLQTATEPSRLFVPIE
                     NPDFNIFVFHPAKTRAPGKTDYLVAALSPVELLRESLTTVDHDQIQIPVHFVDLSASG
                     APRLVASWPDLPEYHNLRPATLLSSAELYPSYVTPVFVFGRSYAIICSAGKGFLAAHA
                     SSPAVMVTIIGILLTLLLSFFAWHLLTERIRAEQTVVLRTLELRQKTEELLLLSENIE
                     TQIWYMKDSETYGGSANRSHRLFLGLLGESLDGKSVRDLVPGESARTFIDGNIEAFAG
                     GRRTYREEWHALPGHSPRLLGITRIPRIGHERTVEYVICSAEDITSRRANENRIERLA
                     ERLTLASEATGVAIWDLDIASGKINYDERLMRLYGLESLDEDLMAIWKSRIPGENAAR
                     TIRAIQNAIESDSEYSGEFTIQHPNGRRIDIEARGIIIRDSLGNPVRMVGTNQDISDR
                     KRAESMLKAREVRFRLLVDNAPFPVLLRDMDGTILFGNNQALTAFETNPLELVGKNIR
                     SLYESGETFDELDGMLPANGQIRNIEAILKTKKGNTFWAIVSANQTEYDGRRVIIATY
                     HDISARRAAEADMKLAKESAEAASRAKSEFLAHMSHEIRTPLNGIIGLSDLMTTTNLD
                     AQQRQYLDSIRISAWSLLDVINDILDFSRIESGKIELEEIPFDLHEIVQNAMRIISSK
                     ASEKGLITEYIISPELPRHTHGDPVRLRQILVNFLGNSVKFTTEGNITLKVRPGPAGM
                     TDFLVQDTGIGIEQEKLHSIFESFSQEDTSTTRQYGGSGLGLAISKRLAAMMHGQILV
                     ESTKGKGSTFTLRLPLKAAGKSPDNEMIDARSVRHGQDIPGKKRFSRQISVLLAEDND
                     INLLVARGNLEACNCRVTPAANGEEAVRIACEASFDLIFMDIHMPRLDGREAARAIRQ
                     FEEKTGRKRIPIIALTADAIQGEENRCLDAGMDDFVTKPFRPDQLTASIEKHTGEHAM
                     IVNDAGIGQPAVPENPATIFDSASLSALLHNDRTAISAIVSMFVKTWEDYIGPIRVAI
                     AHRDLEVLTRQAHRLKGASGNLRADEVFGLAKLLEEQSRAGAEAAVLEQTLHNLEVAW
                     GRLEPELKNLIAE"
     gene            10901..11596
                     /locus_tag="PLD82_00295"
     CDS             10901..11596
                     /locus_tag="PLD82_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009442795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG63858.1"
                     /translation="MDLTLLDAVDSEFLGLNLSLILRSFPLLLQGSLITIEITALSVG
                     FGLIIGMFVGIGRLAHNPVARILSGIYVNFLRGTPLLVQIFIIYFLLPIALGQRVDPF
                     VAAVTACSINSGAYIAEIFRAGIQSIDKGQMEAGRSLGMSWSLTMRSVILPQAFRRIV
                     PPLGNEFIAMLKDSSLVSVIGFEELTRRGQLIISTTYGTFEIWFSVAVIYLVLTFSIS
                     RLVKYLERRLTPG"
     gene            11593..12312
                     /locus_tag="PLD82_00300"
     CDS             11593..12312
                     /locus_tag="PLD82_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOG63859.1"
                     /translation="MIHIHDLHKRFGHLEVLKGVDFHVEPSEVVVVIGPSGSGKSTLL
                     RCINHLEVPTKGEVVVDGIPLTSAENINRVRAEVGMVFQRFNLFPHMRVVDNITLAPI
                     RVRGLSPEEAGERARVLLDKVGLGDKIRAWPDQLSGGQQQRVAIARALAMQPKVMLFD
                     EATSALDPEMTKEVLGVMKDLAREGMTMVVVTHEMGFAREVGHRVLFMDDGRVVEEGV
                     PGQIFDAPVHERTRAFLSKVL"
     gene            12431..13480
                     /gene="hydE"
                     /locus_tag="PLD82_00305"
     CDS             12431..13480
                     /gene="hydE"
                     /locus_tag="PLD82_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOG63860.1"
                     /translation="MTQARGNGASGIDEVIHLLLQRGEPGRLVKEEADALRARTFGDS
                     VHLRGIIEISNRCSKNCAYCGIRAGNSAVQRYTMAEDEIVAAARAAALGGCQTIVLQS
                     GEPPAMGDAALERVIGRIDQECGVAITVSCGVRPEEVYAAWRAAGMRRYLLRFETSDP
                     DLFTRLHPDATLTERLAALETLRGLGVQVGSGFLIGVPGEEVETLARNILLCRDLDLD
                     MIGVGPFIAHGGTPLAGVPNRWADDPEMYFMVVAVLRLVQERAHIPATTAYDALFPRD
                     GRERLLRCGANVFMPNVTPQPYRAAYQLYPGKPCTDEASGACLGCARLRIEALGLRIA
                     EGAGDAVTGGHEWRS"
     gene            13559..15307
                     /locus_tag="PLD82_00310"
     CDS             13559..15307
                     /locus_tag="PLD82_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOG63861.1"
                     /translation="MSVIETLLQILGSLGLFIYGMKVMSDGIQKAAGEKLQKTLGLMT
                     KNRVSALCIGLGVTTVIQSSSATTVMVVSFVSAGMLTLVQAVGVIMGANIGTTVTGWI
                     VAIFGFKMKIAALALPAIGLGLPLLFIKKLKKENFGEFLIGFGLLFLGLSYLKDSMPD
                     IQSNPEILELVKVFSGSGILSHLAFVVIGAVMTVVVQSSSATVAITQTMAFQGWIDFP
                     SAAAIVLGENIGTTITAQLASIGTPLDARRAAMAHTMFNVFGVIWVTAVFPVFIRMVD
                     SMVPGTLFGTIMDTSITGGAAVFHNYLGPVTDGVGLATGIALFHTMFNVTNTFVMIWF
                     VPQFTYLVMKLVRGKREPVEDGVYKLKFLTASLQDTPEMNIAQARKEVQKMAGIVVEM
                     FTRFSEIMHNRETEYEEDVKRFAEKEELTDQMQSEISRFLGECSRYNISDNSASDVAA
                     LIRITDELESIADSCYRLILLAKKRGEKKYEYAKGVVEETKPYTDLVREFLAFINRHL
                     GSSMTQSELDKAADMEARIDEYRNAFKKAARKRIEHGASVRGEILFIDKLQQMEHIGD
                     FAFNIARALSSMNHEH"
     gene            15331..15708
                     /locus_tag="PLD82_00315"
     CDS             15331..15708
                     /locus_tag="PLD82_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63862.1"
                     /translation="MVRTGCALTGILAGLILAACTGGPEYKGDLRDITDVPRTVVERE
                     QRLLEERVNGWARSLDSLQRDNKVRQPIEDNRTSNVAGWVDSGRWVLAMFEAKLAGGP
                     ATVSFTRKEWGAAEDVYCRLVRE"
     gene            complement(15715..16185)
                     /locus_tag="PLD82_00320"
     CDS             complement(15715..16185)
                     /locus_tag="PLD82_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63863.1"
                     /translation="MPRILPTLIAFAVISACSSGPAPWDVLVPSDWLATQTPPGTKSA
                     WIAPDGTATAVISELSSDLDADEFLSAWKTRTPHLAVTKAVEDRGIHLARRFRASSVA
                     TVHLSVNRNGKPASLRAFAFQQGNRVVLLEITATQGPVRSFQDGSRIAESFRFR"
     gene            16332..16757
                     /locus_tag="PLD82_00325"
     CDS             16332..16757
                     /locus_tag="PLD82_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005703350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOG63864.1"
                     /translation="MTVWKKEFTLEGLAETAGGTMTGHIGIEFTDFGDDWLEARMPVD
                     ERTVQYMGILHGGAAAALAETVASYAANLALAEGCAVGLELNISHIRAVRSGYILSRA
                     RAAHIGQSVQVWEIENSRESDGKPVSRARLTMMNLSASD"
     gene            complement(16749..>17573)
                     /locus_tag="PLD82_00330"
     CDS             complement(16749..>17573)
                     /locus_tag="PLD82_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG63865.1"
                     /translation="PTSTHQDLTFWLALAIPGLLLFLGWRLFRNNRRLRETLTRAQRL
                     ASIGTMAGAVAHEINNPLMSMINLAQVIADRSQDAGLRDLANEIITEGERIAKISHNL
                     LDFTRSTHSAVEICDLTSIVRDAVSLTERLFEKQGISLRVSGLDQSALAQCHPGEIRQ
                     VILNLLCNARDALNERYPESHENKSIEIALERSATTAEILLRVSDTGHGIPHHIAPHI
                     FKPFYTTKPEHSGTGLGLAITREIVTRHGGSISFTSSPDEGTSFLVLIPEHPALSV"
BASE COUNT         3522 a         4662 c         5428 g         3961 t
ORIGIN      
        1 gtctgtgcac catggggccc ttggccggcc ctgagaatgt caccatagcg gtggttggtt
       61 cagggttgcg gcttgttccc cgtcccctcc tgttggccca gctcagttcg gaggcgggcc
      121 atgcgggctg cataccgact ggaaggggga cagctccgtg caaattcatc cgagagctgc
      181 cgcgcccgca ggctgtccca agccttcagt gccgaggatg ccatcagcca gagttgttca
      241 tcaagcggaa acatgccgac agttacttgg agcatggtag tcctgcgatt ccgctcggtt
      301 tggacctgca tcaccgttcc gggaagtctg gaccggatat cactcatcag ttgttcggtg
      361 gatgtcgcac gttttccgtc gaccgaacgg atgtagtcat cgacacgcat tccggcggac
      421 tcggctgtcg acccctggat gacatctgtc acgaggatcc cttccatagt atccgtgatt
      481 tttgcaccga tgatgcccca tgggcggaag atgggctcgt tggttggcgt ggttttattt
      541 tgggccggac tggaacaggc gctcagggcc aggaatgcgg agaggatcag ggatattcgc
      601 agaatcatga tggtcggctt ccttcaagac gggactttga gtatcctatc ggagcgttgg
      661 tggccggcat tgatgccttt gcttcatgga ctccattgtg tgaaaaagtg gggcaagttc
      721 gtggagggct tgtatcttgt agctatggcg gaacggctgg atgatgtggc ccgagtggcg
      781 ttgatccgtg agggaaatgc tctcttcaac gaggggcgga ttgaggaagc caagcccaaa
      841 ttcatccggg cagggtacat cgacgggatc atccgtgtgg ctgatcatta ctactacact
      901 ttgaagaaac cggccgctgc cttgatcctt taccggcatg caggttgcac ggccaagtcc
      961 gaagagatca tcgaaacgat tgtggctgtc atccggatcc tcctggccga agatggagca
     1021 gacgaaggga gcgggcgtgg cgaagcgcag ggatgagggt ctttttggcg gtgatcttgg
     1081 tgaacagtcg gcccccctgg ccaggcgcat gcgtccccgt tcgtttgccg agtttgccgg
     1141 gcaggcccac gttttgggac cggggaaacc gctcagacgg tttgcggagg aggggaggca
     1201 tttctcctgg atcatcttcg gccccccggg gtgcgggaaa tccgcctttg cctggctggt
     1261 cgccgccagt gcgcaggccc atttcgagtc catcaatgca gtcacctcca atgttaccga
     1321 actcaaagcg gcgatagagg cagcgtcttc ccggttgcgc tatgagggac ggcggaccct
     1381 gctcttcatc gacgagattc atcgtttcaa ccggatgcag caggatgccc tcctgcccgc
     1441 ggtcgaggac ggaacggtac acctctatgg ggcaacgacc caaaacccgg cattttgcct
     1501 gaacccggcc ctcctttcac gctgccagct tgtcgagttt cgggcattga ccagtgcgga
     1561 tctggcacct ctggttcgaa gaacgctcga ggacgaggag cgtggtcttg gttcacaaga
     1621 gctgaccatt caggatgatg ccctggagct tttggtcgca gcatcctccg gggatgcccg
     1681 ccgtctcctc aatcttctgg aactggcggc ggcttcgctg gaaccccagg gaacgatcac
     1741 cgtggagaca gtcagggatg catcagccgg gcaggccgcc ccggtctatg atcgcgacgg
     1801 agacaaccat tacgatatca cttcggcatt catcaaatcg atgcgcggtt cggatcctga
     1861 tgcggccctg tattggttgg cccgcatgct ggaggggggc gagaatcccg attttatcgc
     1921 ccgtaggatc atgatcgcag cctccgagga tgttggactg gctgattcgg gggccctctc
     1981 cgtcgcggtt tctgccgccc aggcgacggc catgctcggg atgcccgaag cgaggatcgt
     2041 gctggcccat gccgtcctca ggattgccct ggcccccaag agcaactcgg cctgtgtcgg
     2101 aatcgacgct gcactggctg acctgcgagc caatgggcca ctccccgtgc cggagcatct
     2161 acgggatgct cactatgccc ttgcaaaaaa gcttggccat ggagtggaat accggtaccc
     2221 gcatgacgca cccggacatt ggctggtgca ggaatacttg cccgaggctc gcaggtttta
     2281 caaaaattcg ggtcaagggc atgaaggacc gttgcagaaa tggctggatc tgcttcgcag
     2341 ttcggtgaaa ccggacaaca ccgaagggac gtgaggagca ggtacgattc tccggtggca
     2401 ggtccttcag gaagaaattt gttcgtagcg aaaaagaatt tttgtgtcat ttctgcatcc
     2461 gggtatgcgt agccaagtca gtcatggtat agtcttgatg acggtgttag attaatcctg
     2521 tgaacggttc tgccactgcc cggaatgcgg cggtcgccgg accggaacga ccggtcttgc
     2581 agaccgtgtg cgtattgctc cgaaggtgct ggagagggaa tggcgtacga caagcaggca
     2641 atagatgcgg cgcgcaagag cggcatgctg gagagcagca tggatgcgtc cgcgctctct
     2701 cttgacgatc aggagaaggc cgcgcttcgt cgccgacttg agagtgcatc cgccggcatg
     2761 gattccggag atgatgagga tgaagagtcc tctggtcgtg gagaaaatat ttatgagcgg
     2821 ctgcgccggc gcgatgccga atctgcagta ccagccggtg gcgagagccc ggccgggctt
     2881 gcagggcttt tgaccaagat catcatcttt ttcaaatccc tcttctccag tgtctccaaa
     2941 gaggcgatga ccaaaaaaaa agcgttcaag gaccttgaac ggaatctcca ggaatttccc
     3001 atccctgtct atgatcccaa gatgacggca gtccggaaga cattcatcga ctatctggca
     3061 ctgatccaga agaaggtcga aagtttttcg gattttttca gcgccaattt tcttgtcagt
     3121 gccgacgatt ttgtcagtga ccgctcgccc tgtttcgtgc ggttcgtcat cgagacccag
     3181 ctttcgatgg agcaggcccg catgctgcgt gagctcagga cctttgatct gcagcggagt
     3241 ttctccgtgc gcggggagga gattacccag cgggatactg aacttctgat caaaaaattt
     3301 gtcgaatcct tcgatggcgc caatctgcag cgcattgaag agacactcga gttttatgtc
     3361 gccgtactca atctcaaaaa ctacaatttt cagggcatat tcagcctctt tcaggtcgat
     3421 ggcgccaagg acggtgtgtt ccgtgatttc tcgctggagc actgtctcgg acagttgcgc
     3481 aatctggatt cctatcttgc cgggatcaat ttcaacaagc tccgtgagga ccattacgtc
     3541 tggtttgaca ggttccgcga gagtatcgag ggcgggatgg agatcagtgt tccccgctat
     3601 ctctccgaag atttccggac catgatcggg ccactgcgca aactctgcga taaaggggcc
     3661 gtgaccactc ttgtcaggat cgggagcgaa gagatcgggt acaagcccaa gccggttgtc
     3721 aacacgctct cctgggtgga aaaataccgc aaactggcgg agtcgagctt caaggaccat
     3781 cttgaacgga acatcatcct cctcagggag gagcagacgt cggcccaggt cggcgatctt
     3841 tttgaaggct acaggccgtt cacatggctt ccgctcctca atcgtgatac caatgaccgc
     3901 ttgaagcgtg cgggtggtcc gcaatttgcg agcgtttttt tgttcaacct tgtctggaac
     3961 tttttagaca ggatatatat cgaacgcttc aagcgcagcg tgaacagcgt cgttgtggaa
     4021 ggcgagttcc gcaaaaagga gattgctgcc gattttgcca acacgtatta tgcactcgat
     4081 gacttttatg agcgaatgaa gcagatgctc gagcgactga cccccgaatc gtccatcgct
     4141 atccggatca acgggtatgt cgagggcaag atggagaacc cgattgccgc caaaaaattc
     4201 gaggccgatc ttgaggatct ggatgccgag ctttcggaga tggcagagca tgccggaatc
     4261 cagctgatga agatgcaccg ccacatggat tccattctcc gggatttcaa ggggttgcgt
     4321 gcccttgacc tgatcaatgc caaggccatc ggaggtgtct cgaacaggaa tctgatgttt
     4381 gtttatgaca aactggtagt atctttcaaa aagctcgaaa cgattctcgg gcatttcatc
     4441 gttgtcaggg aacagattcc cggatccgga gttccacagg catgagttca tcccgtcttc
     4501 gtctggtttt tatcggaatt gtatcggcca tttttttggc catgctggtt gcctggggga
     4561 gtgctgtcgt tccttttgtg atcggcatcg tgatcgcgta cattctcgac ccctttgtag
     4621 tctttctcca gcgtcggctt catctgccca ggacgcttgc ggtcctgttt gtctttctgt
     4681 tgtttctcgt tgtcttcgtt ctcctgatcg ccttcctcta tccgatgctg gccaatggtg
     4741 tttcatcaat aaccaggacg gttgccgata acgaagcaaa aatcacgctc ttcctcaagg
     4801 atgctgaaaa ctggatcgaa cagctcgatc ttccgttcgc gatcgacagt ggcaaaatga
     4861 tcacaagcat gttcgccgag gcagagacct ttatcaagca ttttttcgga gaactctctt
     4921 cctttgccat ctcgattgta ggcagccttc cgctcctgat cctgattccc ctgatcattt
     4981 tttaccttct caaggacaaa gagaagattt ttgatgttct caagaagtac acaagggatg
     5041 acaacgaggc ccggatccgg attgtgtttg acgaagtcaa caccagactc gggggatatg
     5101 tcaaagggca gttcttcctc tcctggattg tggccaacat caccacggtt gccatgctgc
     5161 tcttcgtggt gccatatgcc gtcctgatcg gtctggccaa cggtgttctc aacatcatcc
     5221 gctatttcgg gcctgtcatc ggagccgtgc cggcgatcat cctcgagctt gtcagcttta
     5281 catcgaccgg acattttctc ggggttgtct tcttctttgt catcatgaac attcttgttt
     5341 cgacaatact ctcgcccaag atctttgccc atgcaacaaa cctccatccc ctggttgttc
     5401 tcctcgcact ctttgtcggg tcgactgcca tgggcatgat ggggatgatc gttgccattc
     5461 cggtggctat catccttcag acattgatcc gcatcgtctt tgatacctat atcaaggaaa
     5521 tctgagacta ttcggcaatc aggtttttca attcgggttc aagacggccc caggcaactt
     5581 cgaggttgtg cagcgtttgc tcgagcacag ccgcctcggc tccggcccgg gactgttcct
     5641 caagaagctt tgccaacccg aagacttcat ctgcccgcag gtttccgctc gcgcctttca
     5701 gacggtgcgc ctgtcgggtc agaacttcaa ggtcacggtg tgcgatcgca acgcggatcg
     5761 gcccgatgta gtcctcccag gtcttgacga acatggagac gatagcgctg atggccgtcc
     5821 ggtcgttgtg caggagggcc gaaagggatg cgctgtcaaa gatggtggcc gggttctccg
     5881 gaacagcagg ttgtccgatg cctgcatcgt tcacaatcat ggcgtgttct ccggtatgtt
     5941 tttcgatcga ggcagtcaac tggtccggtc tgaatggctt ggtgacaaaa tcatccattc
     6001 cggcatcaag gcagcggttc tcttctccct ggatcgcatc agcggtcaat gcgatgatcg
     6061 gaatccgctt tcgccctgtc ttttcttcaa actgccggat ggcccgggcc gcctccctgc
     6121 cgtccaggcg gggcatgtgg atgtccatga agatcaggtc gaaggatgct tcgcaggcga
     6181 ttcgaacggc ctcctccccg ttggcagccg gagtcacccg gcagttgcag gcctcgagat
     6241 ttcccctggc tacgaggagg ttgatatcgt tgtcttcggc gaggagaacc gagatctggc
     6301 gggagaagcg tttcttcccg gggatatcct gcccgtgccg cacgctccga gcatcaatca
     6361 tctcgttgtc cggacttttt ccggcagcct tgagcggaag ccgcagcgta aaggtgcttc
     6421 ccttcccttt cgtgctctcg acaaggatct gtccatgcat catcgcggcc agtcgcttgc
     6481 tgatggccag gcccaatccg cttcccccat actggcgtgt tgtcgaggtg tcctcttgcg
     6541 aaaagctctc gaagatcgag tgaagttttt cctgttcgat tccgatcccg gtatcctgca
     6601 ccaagaaatc cgtcatcccg gccgggccgg gtctcacttt cagggtaatg tttccctcgg
     6661 tcgtgaactt gaccgagttt ccgagaaaat tgaccaggat ctggcgcaag cggacagggt
     6721 ctccatgggt atggcgcggc aattccggcg agatgatgta ttcggtaatc agcccttttt
     6781 cggatgcttt cgacgaaatg atccgcatgg cgttctggac aatctcgtgc agatcgaagg
     6841 gaatctcctc cagttcgatt tttccggatt cgattcttga aaaatccagg atgtcattga
     6901 tcacgtccag gagggaccag gccgagatcc ggatactgtc gagatattgg cgctgttgtg
     6961 cgtccaggtt ggtggtcgtc atcaggtccg agagcccgat gataccgttg agcggtgtcc
     7021 ggatctcgtg gctcatatgg gccagaaatt cactcttggc acggctcgca gcctcggcgg
     7081 attccttggc gagtttcatg tccgcttcgg cggcccggcg ggcggagatg tcatgatagg
     7141 tggctatgat cacccggcga ccgtcgtact ccgtttgatt ggccgacacg atggcccaaa
     7201 aggtgttgcc ctttttggtc ttcaggatgg cttcgatatt ccggatctgt ccatttgcgg
     7261 gcagcattcc gtccagttcg tcaaaggtct caccggattc gtagagggaa cggatgtttt
     7321 ttccgacaag ttcgagcggg ttggtttcga aggctgtcaa tgcctggttg ttcccgaaga
     7381 ggatggttcc gtccatgtcg cgcaggagga ctgggaaggg tgcgttgtca acgaggaggc
     7441 ggaagcggac ctcgcgggct ttgagcatgg attcggcccg tttccggtca ctgatgtcct
     7501 ggttggtccc gaccatgcgg accggatttc caaggctgtc ccggataatg attccccggg
     7561 cttcgatatc aatccgcctc ccgttcgggt gctggatcgt gaactcacca gaatactccg
     7621 aatcgctttc aatcgcgttc tggatggccc ggatggtccg tgctgcgttt tcgcccggga
     7681 tgcgggattt ccagatcgcc atcagatcct cgtcaaggct ctccagtccg tagagacgca
     7741 ttaggcgttc atcatagttg atcttccccg aagcaatatc cagatcccag atggctacac
     7801 cggttgcctc gctggccagg gtcaggcgct cggccaggcg ctcgatccgg ttttcgttgg
     7861 ctctccgcga ggtaatatct tctgccgagc agatgacata ctcgacggtg cgttcatgtc
     7921 cgatacgggg gatacgcgta atgccgagga gccggggtga gtgacctggc agcgcgtgcc
     7981 attcctcacg gtacgttcgt cgaccgccgg caaaggcctc aatatttccg tcgatgaagg
     8041 tccgggcact ttccccaggg acgagatctc tgaccgattt cccgtccagg gactcaccga
     8101 gaagtcccag aaagagtctg tgtgagcgat tggctgaacc accatacgtc tcgctgtcct
     8161 tcatgtacca gatctgtgtt tcgatgtttt cggagaggag caggagttct tccgttttct
     8221 gccggagttc cagggtccgt agtaccaccg tctgctcggc ccgtatgcgt tctgtcagga
     8281 gatgccaggc aaagaagctc agaaggagag tgagcaggat cccgatgatc gtcaccatga
     8341 cagccggcga tgaagcatgg gctgcaagga agcctttgcc ggcggaacag atgatggcat
     8401 aactccggcc aaacacaaag accggcgtga cgtaggaggg gtagagttcg gcggaagaca
     8461 ggagtgtggc cgggcggaga ttgtggtact cgggaagatc cggccaggag gcgacaagac
     8521 gaggtgcccc gcttgccgag aggtcgacga aatggacggg aatctgtatc tgatcatggt
     8581 caaccgtggt gagtgattca cggagaagtt ccaccggtga aagtgctgcg accagatagt
     8641 cggtctttcc aggtgcccgg gtctttgccg gatggaagac aaagatgttg aagtcggggt
     8701 tttcgatggg gacaaagagc ctggagggtt cggttgctgt ctggaggctg gtcgcggccg
     8761 agatctgcat ggcctcatcc gcaaggatgt ccgttcccaa gtccaggcca tgcaggtgga
     8821 gagtgtcgtc accaggtgac acgtagtgag ccggccagta ttcacggcgg aggccagccg
     8881 aaaacatctc atgccggtca ttccgttcct tgaaagagta gtcctgaagc cctgcggctc
     8941 gcccttctgc ttcgaagctg gcccgctctt cccgggatac atggggcgcc cagatcaggt
     9001 agcggaagct cttgcttctt gtgatcagtg gactggcaaa tgcggaaaaa tcctgacgac
     9061 tgacggtttt actgctcgag aaaaagcgct cgagggcctc aagatcatgt gcccgttgca
     9121 gaatcgtgtc atggatcagc cgggtccggt aggccgagag atggtacagc atgtcccggg
     9181 tctcgcgatc agccgctttt tgggtgagga tggccgtcac gatggtcagg actgtgccga
     9241 gggcggcgat cagccagatc tctgtgtgtc gggcaccggt tgaaaagcgt gtctgcctgg
     9301 agcgataccc gagggcgacg aaaccggcca agaggatggt acaggccgcg aacccgagga
     9361 caagggggag gagtcttgcg gtaacgaccg tacttgaacg gagatgatat tcgcgtgaaa
     9421 gttcgatgat cgcgataacg gtctgtgttc tcgggttgat gaccggtgcc cagatgatgt
     9481 cgtgccggtg tcctccttca tccacggtcg cgcaggtggt tgtccggctg ctccatgctt
     9541 gccagaatcc gcggggaatg gtggtacggg tcgatccgct ggccgggaca ggctctccat
     9601 caatggctgc cccggggcca tggatgacag tcgtttcgat cattgttgtc aggatcgctc
     9661 tcgtgtatcc cggtggttga cggatcagga gttcgagatg gcgggcgagc cggttgtaaa
     9721 ccggggttga agcgtctttt cctgtgaatg agagctggag gatgtaactg gatggaagca
     9781 ttgctgcgat tgcctgaccg tggttgcgca gggtctggag ttggcgttcc ccttccatgg
     9841 atcccagaat ctctgccgta caccaggatc ctgcaagaat tccaagcagc agggatgccc
     9901 gcaggagcag tctgcgggag aatggtcctt gtttttcgga ttggtccgag agcttggcgt
     9961 cgattcgccg gatcagcaat acaatcccca gggctatccc catccgcagg acgaggacca
    10021 ggactgcgac ggcatctgcc tgtccggagg gggcgagatt gtatccggac aacgccggga
    10081 taagccaggt cagaacgagg acggggagca gaatgccgag taacttgagg ctctgcccga
    10141 tgccccagcg cgcgatacgt gcttctttgg acagggttcg tgcaacgagg aatgcggccg
    10201 ggaaagcgac aatcagggtg attgcgatct ggagtgccgc caaaccctca aggccgaagc
    10261 tcaaagcgag cggggctggt acccagtaga cagggagggg aagtgcggag tgttgggctg
    10321 cgagagtccg cctggccgat tccaggaggg actgccatgc agccagtgag atcaggatcc
    10381 gcagcacgga aaaaaatgtg gagtcgccca atgccggagt cagggcagag aggagggact
    10441 ccaatccgtg caagacaaag gagagaaaga gcggttgcca ggagaatgcg ggatagcgtg
    10501 tccagatgcc atgggaagac gcgatagagc aaaaagcaag gccgagaagg atatggatga
    10561 aatccgttag catgggcact gtccccggat gtgttcggct tgcttggtac gatctctttg
    10621 taccatggtt caggctgaaa cgcaagtgcg tcccgggaaa tctgtcacgg cacaccgaag
    10681 aatttccttg ctgtgttcag cccatttccg gaaagtcgga gtggtccgcg cgagcctccc
    10741 aaggcggacg tggagggacg ggaatccgga atagcaccac aattctgtaa tacatgggca
    10801 tgaaaggcgt gattgcaaat tcggaccggc tttgtgagta tatttttgtg gagacgaagg
    10861 caaggatggc gtcatggttc ccggcttttt cggactggac atggatttga cactgctgga
    10921 tgcggttgat agcgagttcc ttggactcaa tctcagcctg atcctgcgtt ccttccccct
    10981 tctgctgcag ggttccctca tcacaatcga gattaccgcg ctctccgtgg gcttcggcct
    11041 gatcatcgga atgttcgtcg gtatcgggcg actggcacac aatccggtcg cccgcattct
    11101 ctccgggatc tatgtcaatt ttttgcgggg gaccccgctc ctcgtccaga tcttcatcat
    11161 ctactttctg cttccgatcg ccctcggaca acgggttgac ccctttgtgg cggcggtgac
    11221 ggcctgttcc atcaacagtg gcgcctatat cgccgagatt ttcagggccg ggatccagtc
    11281 catcgacaag ggccagatgg aggctgggcg atccctcggg atgagttggt ccctcaccat
    11341 gcggtcggtc attcttcccc aggccttcag gcggatcgtt cctccgttgg ggaacgagtt
    11401 catcgccatg ctcaaggact cctccctggt ctccgtgatc gggttcgagg aactcacccg
    11461 gcgggggcag ttgatcatct cgacaaccta tgggaccttc gagatctggt tcagtgtggc
    11521 cgtgatctat ctcgtcctca ccttcagcat ctcccgtctt gtcaagtacc tcgagaggag
    11581 gctgaccccg ggatgatcca catccatgat ctccacaagc gttttggcca tctcgaggtc
    11641 ctgaagggcg tcgattttca cgttgaacca tccgaggtgg tggtcgtgat cggtccgtcc
    11701 ggttccggga agagcacact cctgcgctgc atcaaccatc tcgaggtccc gaccaaggga
    11761 gaggtcgtcg tggacgggat tcccttgacc agtgccgaaa acatcaaccg tgtccgggcc
    11821 gaggtcggga tggttttcca gcgtttcaat ctcttcccgc acatgcgggt tgtcgacaat
    11881 atcaccctgg cgccgatccg ggtgcgtgga ctctctcccg aggaggccgg ggagcgtgcc
    11941 cgtgtccttc tcgacaaggt cgggctgggc gacaagatcc gggcttggcc ggaccagctt
    12001 tcgggcgggc aacagcagcg ggtggccatc gcccgggctc tcgccatgca gcccaaggtt
    12061 atgctctttg acgaagcgac ttcggcactc gatccggaga tgaccaagga agtcctcggt
    12121 gtgatgaagg atcttgcccg cgaaggcatg accatggtgg tcgtcacgca cgagatgggg
    12181 tttgcacggg aagtcggtca tcgtgttctc tttatggatg atggccgggt tgtggaagaa
    12241 ggcgtaccag ggcagatctt tgatgccccg gttcacgagc ggacacgggc ctttctctcc
    12301 aaagtgttgt gatggaggga tggccttttt ttggggaaca acctcatgtc cggacagggc
    12361 cggggatcct tggagtgttt ctgtcccgcg cagcgtgcgt gcggactgag tgaccggaaa
    12421 ggcggtaggg atgacgcagg ctcggggaaa tggtgccagc ggtatcgatg aagtcatcca
    12481 tctcctgctg cagaggggag agccgggccg gcttgtcaag gaagaggcgg atgcgcttcg
    12541 ggcgcggacc tttggcgaca gcgtccacct ccggggcatt atcgagattt ccaaccgttg
    12601 ttccaaaaat tgtgcctact gcgggatccg cgcggggaac agcgcggtcc agcgctacac
    12661 catggccgag gacgagatcg tcgccgctgc ccgggccgca gccctggggg gctgtcaaac
    12721 catagtgctc caatccgggg aaccgccggc gatgggagat gccgcgctgg agcgcgtcat
    12781 cggccggatc gaccaggaat gcggtgtggc gatcacggtt tcctgcgggg tgcggccgga
    12841 agaggtctac gcggcctggc gagcggccgg gatgcggcgc tatctcctgc ggttcgagac
    12901 cagtgatccg gacctcttta cccgcttgca tccggatgca acactgacgg agcggctggc
    12961 cgcattggag accctgcgcg gtcttggggt gcaggtcggg agcggcttct tgatcggtgt
    13021 tcccggcgaa gaggtcgaaa cgctggcccg gaatatcctg ctctgccggg atctcgacct
    13081 cgacatgatc ggagtcggac cttttatcgc gcacgggggg acaccgctgg cgggggttcc
    13141 caaccgatgg gccgatgatc ccgagatgta cttcatggtg gtcgctgtgt tgcgcctggt
    13201 gcaggagcgg gcccatatcc cggcgacgac agcctacgat gcgctctttc cccgggatgg
    13261 gcgggaacgc ctgctccggt gtggagccaa tgtctttatg cccaatgtca cgccgcaacc
    13321 gtatcgggct gcctatcaac tctatcccgg aaagccctgc actgacgaag cgagcggggc
    13381 ctgtctgggc tgcgccagat tgcgtatcga agcgctgggg ctgcggatag ctgaaggagc
    13441 cggcgatgcc gtgactggtg ggcatgaatg gagatcttga cgacccttta cggggacggc
    13501 tatggtattg gtgcgggtgt ccccggtacc gtgtgcaatg aaaggggtgt gcttcggtat
    13561 gagtgtaatt gaaaccttgt tgcagattct cggaagtctc ggactcttca tctacggtat
    13621 gaaggtgatg agtgacggga tccagaaggc cgccggggag aagctgcaaa aaaccctcgg
    13681 attgatgacg aaaaaccggg tttcggcgtt gtgtatcggg ctgggtgtga cgacggtcat
    13741 ccagtcctcg tctgctacga cggtcatggt tgtcagcttc gtcagtgccg ggatgctgac
    13801 actggtccag gccgtcggag tgatcatggg cgccaatatc gggacaacgg tcacgggctg
    13861 gattgtggcc atcttcggtt tcaaaatgaa gatcgccgct ctggcgctgc ccgccatcgg
    13921 actcggtctc cccctcctgt ttatcaaaaa gctcaagaaa gaaaatttcg gcgaatttct
    13981 gatcgggttt ggtcttctgt ttttgggact ttcgtatctc aaggacagca tgccggatat
    14041 ccagagcaat cccgagattc tcgagctggt caaggtgttt tccggttccg gaattctgtc
    14101 gcatctcgcc tttgtcgtca ttggtgcggt catgacggtg gtcgtccagt cctccagcgc
    14161 aaccgtggcc attacccaga cgatggcatt ccagggctgg atcgattttc cgtcggcggc
    14221 cgccattgtt ctgggtgaaa acatcgggac gaccattacg gcgcagctgg cctccatcgg
    14281 gacaccgctt gacgccaggc gggcggccat ggcgcacacc atgttcaacg tctttggcgt
    14341 gatctgggtg acggctgtct ttccggtatt catccggatg gtggacagca tggttcccgg
    14401 gacacttttc gggacaatca tggatacgag tattaccggt ggcgctgccg tcttccacaa
    14461 ctatctcggg ccagtgactg acggggtcgg tctggcgacc gggattgccc tcttccacac
    14521 catgttcaat gtgacaaaca ctttcgtgat gatctggttc gtcccccagt tcacctatct
    14581 tgtcatgaag ctggtccgcg gaaagcgtga accggtcgag gatggggtct acaagctcaa
    14641 attcctgaca gcctccctgc aggatactcc cgagatgaat attgcccagg cccgcaagga
    14701 agtgcagaag atggccggga tcgtggtgga gatgtttacc cgcttctcgg agatcatgca
    14761 taaccgggag accgaatacg aagaggatgt caagcggttt gccgaaaaag aggagctgac
    14821 cgaccagatg cagtccgaga tctcgcgctt ccttggcgag tgctcccggt acaatatcag
    14881 tgacaacagc gcatcggatg tggctgccct gatccgcatc accgacgagc ttgagagcat
    14941 tgcggattcc tgctatcgcc tgatcctgct cgccaaaaaa cgaggtgaaa agaaatacga
    15001 gtatgccaag ggtgttgtcg aggagaccaa gccctatacc gatcttgtcc gcgagttcct
    15061 ggccttcatc aacaggcatc tcggctcgag catgacccaa agcgaactgg acaaggcggc
    15121 cgacatggag gcccggatcg acgagtaccg gaatgccttc aaaaaagcgg cccggaaacg
    15181 gatcgaacac ggtgcgagtg tccgcggaga gatcctcttt atcgacaagc tgcagcagat
    15241 ggaacacatc ggggattttg cgttcaatat tgcccgcgcc ttgtcgtcca tgaatcatga
    15301 gcattgaggg gtggaaacaa ggagaagagc atggtacgga ccggatgtgc tttgacaggg
    15361 attttggcgg gcctgattct ggcagcctgt accggagggc cggagtacaa gggcgatctc
    15421 cgggacatta ccgatgtccc cagaacggtg gtcgaacggg aacagcggct cctcgaagaa
    15481 cgggtgaacg ggtgggcaag gagtctcgac tccttgcagc gggataacaa ggtccggcag
    15541 ccaatcgagg acaaccggac atcgaatgtt gccggatggg tcgacagcgg acgatgggtg
    15601 ctcgcgatgt tcgaagccaa actcgcgggc ggtcccgcaa ccgtcagttt tacccgcaag
    15661 gagtggggag ccgcggagga tgtttactgc cggcttgtcc gcgagtgagg gccgtcaacg
    15721 gaatctgaag gattccgcga tccgggagcc gtcctggaac gatcggaccg gaccttgtgt
    15781 ggccgtgatt tccagcagga caaccctgtt tccctgctgg aaggcaaaag cccgcaggga
    15841 tgccggtttc ccgttccggt tgacgctgag gtggacggtg gcgactgatg aagcgcgaaa
    15901 cctgcgggcg agatggattc cgcgatcctc gacagctttg gtgacggcca gatgtggcgt
    15961 gcgggttttc caggcggaga gaaattcatc ggcatcgaga tccgaagaga gttccgagat
    16021 gacggcggtc gcggttccat ctggcgcgat ccaggcggat ttggtgcccg ggggagtctg
    16081 tgttgcgagc cagtccgaag ggacgaggac gtcccagggg gctggcccgg atgagcaggc
    16141 gctgatgacg gcaaaagcga tcagggtggg taggatacgc ggcatgggtg ttcctcctcg
    16201 ttgtctggat gcagtctagc acgggatcca cgtccgggaa tgccgaattt tatcagggtt
    16261 ttgcagacac cggcacggga ttccgggcgc gtgactgtga tttcagggat gtttgccaag
    16321 gggggtaggg tatgactgtc tggaaaaaag aatttacgct ggagggattg gctgaaacag
    16381 ctgggggaac catgaccggc catatcggga tcgaatttac cgattttggt gacgactggc
    16441 tcgaagctcg catgccggta gacgagcgca cggtccaata tatgggaatc ctgcacggcg
    16501 gggcggccgc cgccctggcc gaaaccgtcg cgagctatgc ggccaacctg gcgctcgcgg
    16561 aggggtgcgc tgtcggattg gaactcaata tcagccatat ccgcgccgtg cgctcggggt
    16621 atattctttc cagagcgcgg gccgcccaca tcggacagag cgtccaggtc tgggagatcg
    16681 aaaacagtcg cgagagtgac ggaaagccgg tctcccgtgc ccggctgaca atgatgaatc
    16741 tttccgcgtc agactgaaag cgccgggtgt tcaggaatca ggaccagaaa acttgtaccc
    16801 tcatcggggc ttgaggtaaa gctgatcgag cctccgtgac gggtgacgat ttcccgcgta
    16861 attgccaacc cgagaccggt cccgctgtgt tcgggtttgg ttgtgtaaaa tggtttgaag
    16921 atgtggggag caatatgatg cgggattccg tgcccggtat cactgacacg gagcaggatc
    16981 tccgctgttg tggccgaccg ctccagggcg atttcgatcg atttgttttc atgagactca
    17041 ggatagcgtt cattcagggc atcacgggca ttgcagagga ggttgaggat cacctgacga
    17101 atctcgcccg ggtggcactg agcaagggcg gactgatcga ggcctgagac acgcaggctg
    17161 attccctgtt tttcaaagag gcgttcggtg agtgagactg cgtcccggac aatggaagtc
    17221 agatcacata tctcaacggc ggaatgggta ctccgggtga aatccaggag gttatgcgaa
    17281 atttttgcga tgcgttctcc ttcagtgatg atttcgttag ccaggtcgcg cagtccggca
    17341 tcctggctac gatccgcaat cacctgggcg agattgatca ttgacatgag cgggttgttg
    17401 atttcatggg caaccgcacc agccatggtc ccgatggacg ccagacgttg ggcccgggtg
    17461 agcgtctccc gcaagcgacg gttgttgcga aacagccgcc agccaaggaa tagcagcaat
    17521 cccgggatag ctagtgcaag ccagaacgtc agatcttggt gcgttgaggt agg
//
LOCUS       DAOSHE010000004        14916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_3291_length_14916_cov_5.546666, whole genome shotgun sequence.
ACCESSION   DAOSHE010000004 DAOSHE010000000
VERSION     DAOSHE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_14916_cov_5.546666"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLD82_00335"
     CDS             <1..208
                     /locus_tag="PLD82_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63866.1"
                     /translation="YRGEKAVEKLEKMLRLAADLRYGRVALMDDDLVLLAVAPETFTE
                     ATMLEMVHEVVWIGERLAQELSGV"
     gene            307..1158
                     /locus_tag="PLD82_00340"
     CDS             307..1158
                     /locus_tag="PLD82_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63867.1"
                     /translation="MSCSSSGSLEDVLDGKVQAPGQIQKIFEDNTLEEMVDALRTRLG
                     QGPGWYGNGTNAARLGAMVRFMCEAPGGTVRVVESFADWMGKKPLDEAARKIFRTVGE
                     TNANGLMTVVRSMDFQSPRPVLPELIAVLATAGDETLFGEFKALLQGYDLAPGLFEAA
                     FLSLSMVRFHDTLNFSEAGLKALAVRRIREEWGGGKVSPEDLDAVTERVRFFLSSRTP
                     MFAVLFLEMMADQALKPGKYAGAGRRRIVEHLYDLVEGMKERIEGRSENEKMTFAGIM
                     SRLYGAR"
     gene            complement(1489..2328)
                     /locus_tag="PLD82_00345"
     CDS             complement(1489..2328)
                     /locus_tag="PLD82_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG63868.1"
                     /translation="MRRVIHTISVIQFWQSSAGSSSHALAKKLSHQAQQLRIKLSFKF
                     AQAVLSGQYVGQANDLLRDLTMEISDRGYLSLLYSLYDQTERNRQTEKNRRASAQSRR
                     ISNLDERLRSASPEKYRRLSEALQEEARLRGLDLPPSMLRDHCHVFRQHFDWSTPHRL
                     KKLLALSEESFHQLMCRFATTLALAASDWKNGIRFDWKRFGYAGFDRADYRMFLGERK
                     RHLDTLGLTHDSDHETIRKQYKALAKQHHPDLGGNPERMRELNEAYAALMSNPFINKP
                     HAP"
     gene            complement(2329..3507)
                     /locus_tag="PLD82_00350"
     CDS             complement(2329..3507)
                     /locus_tag="PLD82_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG63869.1"
                     /translation="MPPGKLTNAVSIAVAFLVPGTVINSPVFDAEGNLLIEEKVPITR
                     EIIDALKQKRIDRIYYVQAGRQGPPSDRKPSAIEPDLLDKKILALSEDVFSEVATSVR
                     ENTFIQTTGVHLLVDLILKNLSAKEILPPEFVSPKPASHGFLGHALHTTVLSMFIALK
                     HGFENTLVKQIGIGAFLHDIGSLKLPREMVDKEEFGSEEENALWKKHPALGFELVKLN
                     GQLSGTVRKIILLHHETMDGTGYPLGINEKDVGYYPYIVGLASAFDCFVSERDFRKPL
                     PLREALLKLISFSGSVFPPYLVQLFVKHVSKTPAVSSSYDIGQFVILSSREIARVLVN
                     IPGRPDKPDVLVVRLSDGSDAIPPWKLSLATDPVRSIAKGISGELQQQLERRYPPGDT
                     "
     gene            complement(3543..3899)
                     /locus_tag="PLD82_00355"
     CDS             complement(3543..3899)
                     /locus_tag="PLD82_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63870.1"
                     /translation="MARETLERRDDLFQQWQLRSWFGEQDIIDPAFEDNLHSVWETLF
                     SSLYDDLAERLNNPKARDALFLEFTNTLFPSALDNYKWYASEKRGSDIGLLAALDRIA
                     TEGWYIELLSRFSQTY"
     gene            4131..5189
                     /locus_tag="PLD82_00360"
     CDS             4131..5189
                     /locus_tag="PLD82_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG63871.1"
                     /translation="MKTVFKSGEAPGSAFFLEKGKVIFMIPGAESFELTTANVIFGAG
                     EVFLTWKDGFSVVRSLTAEAADDAVFKPIPVENLRMQIKHYNVGFNIARQIALELRDC
                     NRVLQRTMDSMSSRQRRSHEYAKVVADIVDSLLGTWKEKRFPSLKTIHDEFAGSLTYK
                     YGVSFRNLASKTVIKLDGEQLGSLLRTYPAGSCVCQQGDEGDELYILESGELGIYVGE
                     NEKPVAMINQPGEVIGEMSLLLREPRSARMKAETECVLSVIRKDDLKSVAEAQPSFFL
                     DIGTTLARRMHQSAEVIQELLATKAADARPDCLIEDPHKDTFVALEKRIAQERHDHDL
                     PALKEIHERLRKARIAIV"
     gene            complement(5265..6140)
                     /locus_tag="PLD82_00365"
     CDS             complement(5265..6140)
                     /locus_tag="PLD82_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOG63872.1"
                     /translation="MNEEQRKFTDLSTRKQLREVVTHVNALQLHLVRHGSELAHDPLT
                     DPSIHGILQEIDHKLDWMADTGHFPGRLPRWKDAYDTIVKMFHSKTARKAILAATNNL
                     WHDMAERAEISVREENFIVRTDDRTTPAGPAFPCDIVLDNIRSAFNTGSIIRSADGFG
                     VHGVFLTGITADTNNPKVQKTAMGAIAGVSIQRHTSLHTLIREKKAAGSTIYALETVE
                     GSGNLMCEKFSYPMCLLLGNEEYGIPEDYLSAADRIIHIPMYGLKNSFNVGVSCAIVL
                     YAARQQWDSDKTSLQ"
     gene            6213..7154
                     /locus_tag="PLD82_00370"
     CDS             6213..7154
                     /locus_tag="PLD82_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG63873.1"
                     /translation="MHIFGPVPSRRLGRSLGIDCVPYKTCNLDCVYCECGATTDQTET
                     RGDWFSEDEILDELRVWLDEHPAPDWITFAGSGEPCLFQGIGNLAKRIKEMTGTRLAL
                     ITNGVLLKDEHVRKEARIFDLVLPSLDAATQDAFVRINRPLPGLDVRDIVEGLIRFRE
                     EFAGSIWLEVFIVPGVNDGADEIQALREAILKISPERIQLNSLDRPGTDDSVAVATLD
                     CLKGIQRMIGLSNVEIISRSAIGSPGEIPPEPEAEKRIVMTLLRRPSTTGDLVSASGL
                     PVSQVQLLLADMKRRGQIEKKIVHGDEFWVSATHGPA"
     gene            7330..9120
                     /locus_tag="PLD82_00375"
     CDS             7330..9120
                     /locus_tag="PLD82_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63874.1"
                     /translation="MSRGLDDTRRSIRIDGMEDTEKKKLFNKFQEHGGEVIKEDPRKP
                     LTIDRDKQRDLAKRKAGSYGIPEPVPPGSRSMAGRVASGKKSGSAIGRFFERFALWFK
                     STTAGVMEGTGGILSQKFLHHLHSESQARLLDLGLLVTPLAHPSAEFRPKLLAAMSKL
                     GSVYFELIVRIDKLYDEKLLQEVTCLYTPASPKSVTPRSVGEPLRELYKRIYILRSFS
                     QSATTAIVRALEIQAKEEKKESVALAKNIQRAKKSLDYVFSELLPKLHIAVLNILKRN
                     LWYGHRDLEDFLELTDEDRIGYITERIARELTEAKNRQQSLLEAKKTEILDRSPSEIR
                     ASSLPDQIRDGLLVMREFPIGKDKIVSGRDNPLSLLDEDSKMYLTEVFFEILDREYSF
                     ILTSNKIKIALDYQAGERKDIRKLLNEGYFALDETRNHIKEYNRIVSERWQTERSPQY
                     TPIQKSQMLHKSDMERTRLDNQTRGRFGNILVRIESALKMLIDDQKGANHFLQNPDEP
                     VHFDISGEGKRRLEGRTVIDAISETWAFVSALRYRLLEGDLSGIGSKVEQVLVYDSEP
                     AQGLLESDEEVGPQAAVQPVQALKNDEGQK"
     gene            complement(9189..9845)
                     /locus_tag="PLD82_00380"
     CDS             complement(9189..9845)
                     /locus_tag="PLD82_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63875.1"
                     /translation="MRFESLRKYAGLVLGLVFFATSLSADSDDFGFKPMRRKLRYVEE
                     FYGLFAQNHLASTDSTEQNIWFLQVALNSPFVHPIQALCVIRTEDEHTQYKRLLRSRI
                     AFLVAQGYLQLGYRYDKEDIYWFNLEFADELSKGFKIAEHYYREAIVYWNEAKRVASE
                     IQRYRRTIRLSGGIIDAIQDEARKIHEGDINYQKIIDFRLRDLEKKQHKLESMLKKAP
                     "
     gene            10343..11284
                     /locus_tag="PLD82_00385"
     CDS             10343..11284
                     /locus_tag="PLD82_00385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG63876.1"
                     /translation="MVGFFSFVFLLVCIGATWLVSGGKSVFDADIAGVSDHDLFYRMF
                     LEDETRANIAGPKGGPDSAGALKLFSYTIGKDDSLSKIAEKTGVTIDALISLNRLSDA
                     HIIRVGQQLTIPNQKGIYHKVQKGDSVERLSRRYSIQAESIQRANGLVRDELEMGSVV
                     FLPGARLSADEMERALGMLFVRPASGGWLSSSYGYRRDPFTLSHQFHPGIDIALPYWT
                     PIVAVRSGQVVFSGWNGGYGKMVMVKHSDGYTTIYGHMIQNTAASGQWVRAGQRLGYV
                     GSTGYSTGPHLHFELRRYGQLINPLQVPGFRRAFIRG"
     gene            complement(11325..11687)
                     /locus_tag="PLD82_00390"
     CDS             complement(11325..11687)
                     /locus_tag="PLD82_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63877.1"
                     /translation="MHFAKAVKGGRIRIENRSNFLARADKTKKAEQYITLSGFDKDWL
                     EKMAFTLKASQTEILRMALEWWKAVEGNNESAKDHWHTDTISERPSGSRFCLWSAGYM
                     TLSHFPTKEEALVASRYS"
     gene            12018..12602
                     /locus_tag="PLD82_00395"
     CDS             12018..12602
                     /locus_tag="PLD82_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63878.1"
                     /translation="MTRLFLLLLLFCLFSWMISSCSREVQGVAPEQIANDFFLKNTQL
                     KKRGVQLSLVSTRTVENAIRYVVGLAKESKNEDMRQWEMEYVRSGSEWVLGSIKEIPC
                     SGKSCESELISKATNMLVKLRDYSSAMVLSNAGFHRYIKNGEQKAEVVLIVRKTGRSE
                     SERIKLIFREESGVFVFNGAYLESLGDYRQIQAQ"
     gene            complement(12796..14391)
                     /locus_tag="PLD82_00400"
     CDS             complement(12796..14391)
                     /locus_tag="PLD82_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOG63879.1"
                     /translation="MAKNTKGYLALVLHAHLPFVRHPEYEDFLEEDWLYEAITETYLP
                     LLDVYEGLVRDGIDFRITMSLTPPLANMLADELLQNRYVKHIRDLIELAEKEVERTRW
                     MSEFHETAKMYLEKFERCRYLFEEVYRKNLINGFKRFQELGKLEVITCGATHGFMPLM
                     DLYPNAVRAQIQVARQDYERHFGRPPRGIWNGECGYYPGLETFLSEAGIRFFFVDSHG
                     ILHADRRPRYGVFAPLYCPNHVAAFGRDIESSKSVWSSEEGYPGDFNYREFYRDIGFD
                     LDFEYIKPYIHESGLRTNTGIKYYRITGRTMYKEPYNRQRAMEMAAEHAGNFMFNREK
                     QVEHLSAVMDNKIPIVVSPYDAELFGHWWYEGPDFLNFLLRKISTSSDTIELITPSEY
                     LDRHPEQQTATPSLSSWGNRGYAEVWLNSTNDWIYRHLHKASERMNELANTYRDAEGL
                     IRRALNQAARELLLAQSSDWAFIMKTNTMVEYAVKRTKDHINRFTDLYYAIKSGTIDE
                     SILTSLERKDNIFPDIDFRVYCD"
     gene            complement(14426..>14916)
                     /locus_tag="PLD82_00405"
     CDS             complement(14426..>14916)
                     /locus_tag="PLD82_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63880.1"
                     /translation="RSNSVLTPRDTLSDRIDEKPEWHTTTETATEMYRISGGYKVTEL
                     AGSESIGALLAQHLAANLSSGAVASSSNVPRKDEGGEKGKDFWLVVNTELIVYGATEP
                     DAKVTLQGQPLKLRPDGTFSVRFALPDGEQIIPVHAVNADGDMEKQITPIVTRTTRVD
                     IP"
BASE COUNT         3504 a         3322 c         4344 g         3746 t
ORIGIN      
        1 ttatcgcggt gagaaagctg tggagaaact tgaaaagatg ctcagactgg ctgccgattt
       61 gcggtatggc cgggttgcgt tgatggatga cgatcttgtc ttgctggcgg tggcaccgga
      121 aacgtttacg gaggcgacca tgctggagat ggttcacgag gtggtctgga tcggagagcg
      181 gttggctcag gaactctccg gagtgtaaaa ccgcttcaag atttgaaaac agattatcag
      241 catgagggat gacaacaagt gatgagcggt ttcaggtgcc tgacggttta tttggtttgt
      301 gtaacgatgt cgtgcagcag cagcggatct ctggaagatg tccttgatgg caaggtgcag
      361 gctccgggac agatccaaaa aatcttcgaa gacaacacgc ttgaagagat ggtggatgca
      421 ctccggaccc ggcttgggca gggcccgggc tggtatggaa atggaacgaa tgctgccagg
      481 ttgggagcca tggtgcggtt catgtgtgag gcacccggcg ggacggtccg tgtggtggag
      541 agctttgccg actggatggg gaagaaaccg ctggacgaag cggcgaggaa aatattccgg
      601 acagttggcg agacgaatgc gaatggactc atgacagttg tcaggtcgat ggattttcag
      661 tcaccacggc ccgttcttcc ggagttgata gcggttcttg ctaccgccgg ggacgaaacg
      721 ctttttggag agttcaaggc tctcttgcaa gggtacgatc tggcacccgg actttttgaa
      781 gcggcatttc tgtctctctc catggtgcgt tttcatgata cgctgaattt ttcggaagca
      841 gggctcaagg ctcttgccgt ccgacggatt cgggaagaat ggggtggcgg gaaggtgtct
      901 cccgaagatt tggatgctgt caccgagcgc gttcgttttt tcttgtcgtc ccggaccccc
      961 atgtttgcgg tgctttttct tgagatgatg gcagaccaag cgctgaagcc gggaaaatac
     1021 gcaggagcag ggcgacgaag gatcgtcgaa cacctgtatg accttgttga agggatgaaa
     1081 gagagaatcg aaggccgaag cgaaaacgaa aaaatgactt ttgccggcat aatgtcgagg
     1141 ctctatggtg cacgataata ctgcaaattt tttgaatgca tcatcctatg gtgcacggga
     1201 gtacggaaat gacaggggcg caccaccatg tggtgcacgg gagtacggaa atgacaggga
     1261 tgcaccacca tgtggtgcac gatgggtttc tgatggctag ttttttctat agtggcgtac
     1321 tgggattgta gaaccgggat ggccccccct atggtgcagt ttcggtttgg catgagccgt
     1381 cctatggtgc attgccccca cccggatgat ctctgacttt gtatgtgccg ggatgaatct
     1441 gcaggctggg ggactatggt gcagttttcc cgtgggatgg tctcttggct atggtgcatg
     1501 tggcttgttg atgaacgggt tcgacatcag ggcggcgtag gcttcattga gttctctcat
     1561 tcgctccggg ttaccaccaa gatcgggatg gtgctgcttg gcgagggcct tgtattgctt
     1621 gcggatggtt tcatggtcgg aatcatgggt caggccgagt gtatcgaggt gacgtttccg
     1681 ctccccgaga aacatgcggt agtcagcacg gtcaaatccg gcatagccaa agcgtttcca
     1741 gtcaaaacgg atcccatttt tccagtctga agcggcgagg gctaacgtag tagcaaaacg
     1801 gcacatgagc tgatgaaagc tttcttcgga aagagcgaga agttttttga gacgatgggg
     1861 cgtggaccag tcaaagtgtt ggcggaagac atggcaatgg tcacgaagca tggacggcgg
     1921 cagatcgagg ccgcggagac gagcctcttc ctggagggct tcagaaagac gccgatattt
     1981 ttccgggctc gcggaacgaa gcctctcgtc aagattggag atgcgtctgg attgggcgga
     2041 agcccgtctg ttcttttctg tctggcggtt gcgttctgtc tggtcataga gggagtagag
     2101 caatgagagg tatcccctgt cagaaatctc catggtgaga tcccggagga gatcgtttgc
     2161 ctggccgacg tattgccccg agagaaccgc ttgggcgaat ttgaacgaga gtttaatccg
     2221 gagttgctgt gcctgatggg agagtttttt ggccagagca tgggaagagg agccggcgga
     2281 agactgccaa aactggatga cggatatggt atggatgact cggcgcattc aggtgtcacc
     2341 tggtggatag cggcgttcga gttgctgctg caattctccc gaaatccctt tggcgatgga
     2401 acggaccgga tccgttgcaa gggagagttt ccatggcggg atggcgtcgc ttccgtcaga
     2461 aaggcgaacg acgaggacat ctggtttgtc tggccttccg gggatgttga cgagaacacg
     2521 ggcgatttcc cgactggaaa gaatgacgaa ctgcccgatg tcgtaactgc tcgagacggc
     2581 cggtgttttg gaaacatgct tgacaaagag ctggaccaga tatgggggaa aaacggatcc
     2641 ggagaagctg atcagcttga ggagtgcttc ccgcagaggg agcggcttgc ggaaatcccg
     2701 ttcgctgacg aagcagtcaa aggcgcttgc aagcccgaca atataagggt aatatccgac
     2761 gtctttttcg ttgatgccga gagggtagcc ggtgccatcc atggtctcat ggtgcaggag
     2821 gatgattttc cggacggttc cggagagctg cccattgagt ttgacgagtt caaaaccaag
     2881 agcaggatgc tttttccaga gtgcgttctc ctcctcggag ccgaactctt ccttgtcaac
     2941 catctcccgg gggagtttga gacttccgat gtcatggaga aatgcgccta tcccgatctg
     3001 cttgaccaga gtgttttcaa aaccatgttt gagcgcgatg aacatggaga ggaccgtggt
     3061 gtggagggca tgtccaagaa aaccatggga ggccggtttg ggcgagacaa attcaggggg
     3121 gagaatctcc ttggccgaga gatttttgag aatcagatcg acaaggagat ggacgccggt
     3181 tgtctggata aatgtgttct cccggacgct ggtggccact tcggagaaaa catcctcgga
     3241 gagtgcgaga atttttttgt caagaagatc gggttcgatt gctgatggtt tccggtcgga
     3301 gggagggccc tgccttccgg cctggacata ataaatccgg tcgatgcgct tctgtttcag
     3361 ggcatcaatg atttcccggg taatggggac cttctcttcg atgaggagat ttccttcggc
     3421 atcgaagacc ggactattga tgacagtgcc gggaacaagg aaggcaacag ctatcgagac
     3481 ggcatttgtg agtttgccag ggggcactgc ggatcctccg tacgacgtgg cagagacaga
     3541 ccctagtagg tctgggagaa acgggagagc agctcgatgt accagccctc ggtggcgatc
     3601 cggtcgagtg ctgccaaaag accgatatcg ctaccccgtt tctcgctggc gtaccacttg
     3661 tagttgtcca gtgccgaagg aaaaagcgtg ttcgtgaatt cgaggaagag ggcgtcccgg
     3721 gccttcggat tgttcaggcg ttcagcgagg tcgtcgtaga gactggagaa gagtgtctcc
     3781 cagacggagt gaagattgtc ttcgaaggcc ggatcaataa tatcctgttc tccaaaccat
     3841 gaacgaagct gccactgctg aaaaaggtca tccctgcgtt caagtgtctc gcgagccatc
     3901 cctgtcctcc ggtaccatga tggagttgag tatacacctg ttttcagttt gaccgcaagg
     3961 gatgccgata atccgcagga gggaccgggc agatgttctt caaggggcgt gtgttggtca
     4021 gagtgttttt tgaggaacaa actcgggccc ggggacgcga ttgggtgtag agtgagggtg
     4081 gtgggaacgg aagcgtgccg gcccggaagt catgccatgg gagaatgtga atgaagacgg
     4141 tcttcaagtc gggagaagcc ccgggaagtg cgttttttct tgaaaaaggg aaggtcatat
     4201 tcatgattcc tggagccgag tcgtttgagt tgacaaccgc caatgtcatt tttggagccg
     4261 gtgaggtctt tctgacctgg aaagacggtt tttccgtagt caggtccttg acagccgaag
     4321 ccgccgacga tgcagtgttc aagcccattc ccgtcgagaa tctgcgaatg cagatcaagc
     4381 actataatgt cggattcaat attgcgcggc agatcgcact tgaattgcgg gattgcaaca
     4441 gagtcttgca gcgaacaatg gattccatgt catccaggca gcgcaggtcg catgagtatg
     4501 cgaaggttgt ggcggatatt gtggacagcc ttctgggaac atggaaagag aaacggtttc
     4561 cgtcattgaa gacgatccat gacgaattcg cgggaagtct gacatacaag tacggggtca
     4621 gtttcaggaa tctggcaagc aagaccgtga taaaattgga cggggagcag cttggttctt
     4681 tgctgaggac gtatcccgca ggaagctgtg tgtgccagca gggtgatgag ggggacgagc
     4741 tctacattct ggagtccggg gaactgggta tttatgtcgg agagaacgag aagccggtgg
     4801 caatgatcaa tcaaccggga gaggtgatcg gcgagatgag ccttctcttg cgtgaaccga
     4861 ggtctgccag gatgaaagcg gaaaccgagt gcgtgctctc ggttatccgg aaggatgatc
     4921 tgaaaagtgt tgctgaagct caaccctcgt tttttctgga tatcggaaca actctggcac
     4981 gcagaatgca tcagtctgcc gaggttattc aggaactgct cgcgacgaaa gcagccgatg
     5041 cccgaccgga ctgcctgatc gaggatccac ataaagacac atttgtggcg cttgagaagc
     5101 ggatagccca ggagcggcat gaccatgatt tgccggcact caaagagatt cacgaacgac
     5161 taagaaaggc gagaatcgcg attgtctagc aagccccgac atgctgggtt tttcaggcac
     5221 aggcgactgc accataggga gcaacctgcg gcagggtggt ttggctactg gaggctggtt
     5281 ttgtccgaat cccattgttg ccgggctgcg taaaggacta tggcacaact gacgccaacg
     5341 ttgaacgagt ttttcagacc gtacatggga atgtggatga tccgatcagc tgccgaaagg
     5401 taatcttcgg gaatgccgta ttcttcattg cccagaagaa ggcacatcgg gtatgagaac
     5461 ttctcacaca tgagatttcc cgaaccttca accgtttcca gcgcatagat tgtcgaaccg
     5521 gctgcttttt tctccctgat aagggtgtgg agcgaagtat gccgttgtat tgaaactcct
     5581 gcaatcgcgc ccattgccgt tttctggacc tttgggttgt tagtgtcggc agtgataccg
     5641 gtgagaaata caccatggac accgaaacca tctgccgagc ggatgattga gccggtattg
     5701 aacgcggacc ggatgttgtc aaggacgata tcgcagggga acgccggtcc ggcaggagtc
     5761 gtcctgtcat ctgttcgaac aatgaaattt tcttcacgga cagagatttc tgcccgctct
     5821 gccatatcgt gccagaggtt gttggtggcc gccagaatgg ctttccgggc tgtttttgag
     5881 tggaacatct tgactatggt gtcataggcg tccttccagc ggggaagacg accgggaaag
     5941 tgcccggtgt ctgccatcca gtccagttta tggtcaattt cctgaagaat cccatgaatt
     6001 gatggatcgg tcaggggatc atgagccagt tcagacccgt gccgaacaag atggagttgc
     6061 agggcgttga catgcgtcac gacttcccgc agttgttttc tggtcgaaag gtcggtgaat
     6121 tttctttgtt cttcgttcat cgtgattaaa gatatctttc gaggagggaa caagtcacgc
     6181 tatggtgcag tttttgttct tctggagggg ctatgcacat ctttggacct gttccgtcac
     6241 gccgtcttgg acgctctttg gggatcgatt gtgtcccgta caaaacgtgc aatctcgatt
     6301 gcgtgtattg tgaatgcgga gcgacaaccg accagactga aacccggggg gactggtttt
     6361 ctgaagacga gattctggat gaactccgtg tttggctgga cgagcatcct gcgccggact
     6421 ggatcacgtt tgccggttcg ggagagccgt gcttgtttca aggaatcgga aatttggcga
     6481 aacgcatcaa agagatgacg ggaacacggc tcgcattgat cacaaacggg gtattgctca
     6541 aggatgagca tgtgcggaaa gaagcccgga tattcgatct ggttcttcct tccctggatg
     6601 ccgcgacgca ggatgccttt gtgcggatca atcggccact tcccggcctt gatgtgcgcg
     6661 atattgtcga aggtctgata cgctttcgtg aggagtttgc cggcagtatt tggctggagg
     6721 tctttatcgt tccgggagtg aatgatgggg ctgacgagat acaggcattg cgcgaggcga
     6781 tcctcaagat ctcgccggag aggattcaac tgaattcact cgacaggccc gggacggatg
     6841 actcggtcgc cgtagcaaca cttgattgtc tcaaagggat ccagcgcatg atcggtttgt
     6901 cgaacgtcga gatcataagc agatcggcca ttggttctcc gggggagatt ccgcctgaac
     6961 cggaagcgga gaagcggatc gtgatgacgc ttctccggcg accatcaacg accggggatc
     7021 tggtgagtgc cagcgggttg ccggtttcac aagtacaact cctcctggca gacatgaagc
     7081 gtcgtggcca aattgagaag aaaattgtcc atggagatga attctgggtc agtgctacgc
     7141 atgggcctgc ctgaattccg gtaaaaatcc ggcgtatcca tggccttcgg ctgttcctcg
     7201 tgaagaatac tggttgaatg atccagtgtc atacagacta caggtatgat tattttacag
     7261 cgtgcaagat tgacagagtg atagagactc aatatgctta gggtttgagt gagagcttgg
     7321 gagggacgaa tgtccagagg gcttgatgat acaaggcgat ccatccggat tgacgggatg
     7381 gaagacacgg aaaagaaaaa gctcttcaac aagttccagg agcatggcgg cgaggtgatc
     7441 aaggaagatc cccgcaagcc cttgacgatc gaccgcgaca agcaaaggga tcttgccaaa
     7501 agaaaagccg ggagttacgg aattcctgag ccggtaccgc cgggaagcag gagcatggcg
     7561 gggcgtgtcg catccgggaa aaagtccggg agcgctatcg gaaggttctt tgagcggttt
     7621 gcgttgtggt tcaaatcgac gacagcagga gttatggaag gtacaggcgg gatcctgtca
     7681 cagaagtttt tgcatcacct tcacagtgaa tcccaggcaa ggttgcttga tcttggcttg
     7741 ctcgtgacac cgctggcaca tccgtctgcg gagttccgtc cgaagcttct tgccgccatg
     7801 tcaaagctgg gctccgttta tttcgagttg attgtccgga tcgacaaact ttatgatgaa
     7861 aagcttcttc aagaggtgac atgtctgtat accccggcct ccccgaagtc tgtgacaccg
     7921 cgttctgtcg gagagccgtt gcgagaatta tacaagagga tctatatcct ccggagtttc
     7981 agccagagtg cgacaacggc catcgtccgg gcattggaga tccaggcgaa agaagaaaag
     8041 aaggaaagcg ttgcactcgc caaaaacatt caacgggcaa agaagagcct ggattatgtc
     8101 ttttcagaac ttcttcccaa gcttcacatt gcggtgttga atattctgaa gcggaatctc
     8161 tggtacggtc atcgtgatct tgaggatttt ctcgaattga ctgacgagga caggatcgga
     8221 tacatcacgg agcggattgc gagggaattg accgaagcaa agaatcggca gcagtcattg
     8281 cttgaagcga agaagacaga gatattggac agatccccaa gcgagattcg tgcttcttcc
     8341 ttgcctgatc aaatcaggga tgggcttctt gtcatgaggg agtttccgat cggaaaagac
     8401 aagatcgtat ccgggcggga caaccccttg tcgttgcttg atgaagacag caagatgtat
     8461 ctgacggaag ttttttttga aatcctcgat cgtgagtatt cattcatcct gacgagcaac
     8521 aagattaaaa tcgcccttga ttaccaggcc ggagagagaa aggatattcg caaactgctc
     8581 aatgaggggt attttgccct cgatgagacg agaaatcata taaaagaata taaccggata
     8641 gtgagtgagc gttggcagac ggaacgatct ccccagtaca cgccgataca gaaatcacag
     8701 atgctgcata aaagtgacat ggagcgaact cgcctggata atcagactcg tggccgtttt
     8761 ggcaatatcc ttgtgcgtat cgaatcggcg ttaaaaatgc tgatagatga ccaaaaagga
     8821 gccaaccact tccttcaaaa tcctgatgag ccggttcatt ttgatatctc cggagaagga
     8881 aaacggcgtc ttgaaggccg gacggtgatt gatgcaatca gtgagacatg ggcgtttgtg
     8941 tcggctttac gatatcgtct tctcgaaggg gatctttccg gtattggttc gaaggtcgag
     9001 caggtcctcg tgtatgacag cgagcccgcc cagggtctgt tggagtccga tgaagaagtt
     9061 gggcctcagg ctgccgtcca gccggtacag gctctgaaaa acgatgaggg tcagaagtag
     9121 gagatttcgg ggcgagcggg gggcgggaaa atgcaccatg gctgtttgtg agcaagccca
     9181 agggtgagct atggtgcttt tttaagcatg ctttccagtt tatgctgctt tttctccagg
     9241 tctctcagcc ggaaatcgat gattttctga tagttgatat cgccttcatg aattttcctg
     9301 gcttcgtcct gtatggcatc aataatgcca ccggagagtc gaatcgttcg gcggtagcgc
     9361 tggatttctg aagcaacacg tttggcttca ttccaataca cgatggcttc tctatagtag
     9421 tgctccgcaa tcttgaatcc ttttgaaagc tcatcggcaa actccaggtt gaaccagtag
     9481 atatcttctt tgtcataccg ataacccagt tggagatagc cttgggcaac aaggaaggca
     9541 atgcgtgatc gcaggaggcg tttgtattgg gtgtgttcgt cctcggttct gatgacgcaa
     9601 agagcctgga tcggatggac gaagggtgag ttgagggcta cctgaagaaa ccatatgttt
     9661 tgctcggttg agtcagttga tgccagatgg ttttgggcaa aaaggccata gaattcttcc
     9721 acatagcgca gttttcgtct cattggttta aagccaaagt catccgagtc tgcagatagt
     9781 gatgtggcaa agaaaacgag cccgagaacc agtcctgcat atttgcgaag tgactcaaat
     9841 ctcacagtaa actcctgatg tgctctcatc ctttggctgt acttcgagta tcggaggatt
     9901 tatatggaat cagtacccta tggtgtgaaa tctggtatgg tgtgcagatg ttttaaaagg
     9961 tgcgtggcat ggatgccagc aactctttta atgccattga tcaacacatg gggattgtct
    10021 gtttccgtca gaatgaaaaa aatcgaaggt tgactatagg ggtaaaaaaa aggtagaatt
    10081 tcgtgtgata aagcggaaga gatagtctgc cgatagtaca ggagaggtgc gtcactatgg
    10141 tgcgcctact tgctgaaaca ggtgggcaaa agtgatcaga gcaggtatgg ctaccaaggg
    10201 aaaaaacttg cgcattgaaa gaccagcgat gtgggggagc cggcagggtg cttcttcagg
    10261 tcgcctgcgg gtgatttatc caaaggtttt tcgacaaggc gtggagttga caagaaaact
    10321 cgagagaaaa tcaccaaggt tgatggtagg cttcttctcg tttgtctttt tgctggtttg
    10381 tataggcgcg acctggcttg tttcaggtgg caaatccgtg tttgatgcgg atatcgctgg
    10441 tgtcagtgat catgatctct tctatcgcat gttcctcgag gacgagacac gggcgaacat
    10501 tgccggtccg aagggtgggc ccgattctgc tggcgcactc aagctgtttt cgtatacgat
    10561 cggcaaggac gactcgctgt ccaaaatcgc agagaagacg ggcgtgacca ttgatgcctt
    10621 gataagcctg aatcggctgt cagatgctca tattatcaga gttgggcagc aattgaccat
    10681 tcccaatcag aagggcattt atcacaaggt tcaaaaaggt gattctgtcg agaggctttc
    10741 gagaagatat agtatccagg ccgagagcat tcagagagca aacggtttgg tgcgtgatga
    10801 gctcgagatg gggagtgttg tttttctccc gggagctcgt ctttccgctg atgaaatgga
    10861 gcgggcgctt ggaatgcttt ttgtcagacc ggcttccggt ggttggcttt cttcctctta
    10921 tggctaccgg cgagatccgt tcacactttc ccatcagttt catcccggga tagacattgc
    10981 cttgccatat tggaccccca tcgtagctgt tcgaagcggt caggttgttt tttccggctg
    11041 gaacggggga tatgggaaaa tggtcatggt taagcatagc gatgggtata cgacgattta
    11101 tgggcacatg atccagaaca cagctgcttc cgggcagtgg gtgcgcgctg gccagcgact
    11161 cggatatgtg ggaagtaccg ggtattcaac aggcccgcat ctccattttg agcttcggcg
    11221 ctatggacag ttgattaatc cgttgcaggt tccaggattc cgcagggcgt tcatccgggg
    11281 ataaattgtg ttggtgtgtc ggtcaagtcg attgttctgg ctgcctacga atagcggctt
    11341 gcgacgagtg cttcttcctt ggttggaaag tgggagagtg tcatgtatcc tgcactccag
    11401 agacagaatc gagaccctga aggccgttcc gatattgtgt cagtatgcca gtggtctttc
    11461 gcactttcat tgtttccctc aacagctttc caccattcga gtgccatgcg aagtatttct
    11521 gtttgacttg ctttaagtgt gaatgccatt ttctccagcc agtctttatc gaagcccgaa
    11581 agagtgatgt actgctcggc tttttttgtt ttgtcagcac gggcgagaaa attgctcctg
    11641 ttttcaatgc ggatcctgcc gcctttgacg gcttttgcaa aatgcatcag tgcgattctg
    11701 caaaatgcgc tcaatttctc ccggtgcgag gcacaccatg tgcgtatatc aatctccatc
    11761 ctgcagtgga agcgaattga accggattca tgcaaccgtt cagggtcatg agttgtgcaa
    11821 tgcatgtgct gactcctctg tttattgtat agaagaggtc tgatgcgctg gattggttcc
    11881 ctcttttttg cctttttctg ccatggtgca gttgtttctg ggacactcat cgcggctatt
    11941 gagaaataaa cggatggttt acaagatgaa acttctgtgt atactgaaga aagatgtaga
    12001 tcgaaaggaa ttgatatatg actcgcttgt ttctgcttct actgctgttc tgtctgttct
    12061 cttggatgat ctccagttgt tcccgggagg tccaaggagt agctccggag caaattgcta
    12121 acgatttctt tttgaaaaac acgcagttga aaaaacgagg agttcagctg tctcttgttt
    12181 caacgcggac tgttgaaaac gcaattcgat atgtggttgg tctggcaaaa gaaagcaaga
    12241 acgaagacat gcggcagtgg gagatggaat atgttcgctc cggttcggaa tgggttcttg
    12301 ggtcaataaa agaaattccg tgttccggca aaagctgtga atccgaactg atctcgaagg
    12361 cgaccaacat gcttgtgaag ttgagggact attcaagcgc catggtgctc tccaatgcag
    12421 gattccatcg gtatataaaa aatggagagc agaaggccga agttgtcttg attgtccgca
    12481 agacagggcg atctgagagc gaacgcatca agctgatatt tcgggaagaa tccggcgtct
    12541 ttgtcttcaa tggcgcgtat cttgaaagtc taggggacta caggcaaata caagcacagt
    12601 agtcaggctg gtatgtgatt cgggtgcgat cgatcaatat ggtatcgggc gccatcatcc
    12661 accacagaag gattgccgca acatacaaca taccgatgag accggatttc tccggactcc
    12721 gtcggtctgt ggcaaccatc acacaaggaa cagagattca atacaggctg tggtgcaaag
    12781 aacggaccgg aatggttagt cgcaatacac cctgaaatcg atatccggga atatgttgtc
    12841 ttttcgttcc agagaagtca gtatggactc atcaatggtt ccgctcttta tcgcatagta
    12901 cagatctgtg aaacggttaa tgtggtcttt tgttcgtttt actgcgtatt caaccatggt
    12961 gtttgttttc atgatgaaag cccagtccga actttgcgcg aggagtagtt cccgtgctgc
    13021 ctggttcaat gctcgccgga ttaatccttc cgcatccctg tatgtgttgg ccagctcgtt
    13081 cattctttcg gatgctttgt gcaaatgtct gtatatccag tcattcgtgc tgttcaacca
    13141 aacttcggca taccctctgt ttccccagct tgaaagcgaa ggcgttgcgg tctgctgctc
    13201 gggatgccga tcaaggtatt cggatggcgt aatcagctcg atggtatctg aactcgtcga
    13261 aatcttgcgc aacaggaagt tgaggaaatc cggcccctcg taccaccagt ggccgaagag
    13321 ttctgcatca tagggcgaaa cgactatggg gattttattg tccatgacag cagagagatg
    13381 ctcgacctgt ttttcacggt tgaacatgaa attcccggcg tgttcggcag ccatttccat
    13441 ggctctctgt ctgttgtacg gctctttgta catcgttcgt ccggtgatcc ggtagtactt
    13501 gatgcccgtg ttggttctca gaccggactc atggatgtac ggcttgatat actcgaaatc
    13561 cagatcaaaa ccgatatctc ggtagaattc acgatagttg aagtctccag ggtatccttc
    13621 ctcggaagac cagaccgatt tggatgattc tatatctcta ccaaaggctg ccacatggtt
    13681 cgggcagtac aaaggtgcaa atacaccata gcgcggtctt ctatcagcat gcaggatacc
    13741 atgtgagtcg acaaagaaga aacgaatccc cgcttcggaa aggaaagttt cgagcccggg
    13801 gtagtagcca cattccccat tccagattcc acggggaggg cggccaaagt gtctttcata
    13861 atcttgacgg gcaacttgaa tctgggctcg taccgcattg ggatacagat ccatgagcgg
    13921 catgaagcca tgagtggcac cacaggtgat gacctcaagt tttccaagct cttgaaagcg
    13981 cttgaagcca ttgatcaggt tttttctata aacttcttcg aagagatacc ggcatcgctc
    14041 gaatttctca aggtacattt ttgcggtttc atggaactcc gacatccagc gcgtacgctc
    14101 gacttccttt tcagcgagtt caatcagatc ccgaatatgt tttacatatc tgttctggag
    14161 caactcatca gccagcatat tcgccaatgg tggcgtgagc gacattgtta ttctgaagtc
    14221 gataccgtcc cggacgagcc cctcatacac atcgaggagg ggaagatagg tttccgtaat
    14281 tgcctcgtat agccagtctt cttcgagaaa atcttcatat tcaggatgac ggacaaacgg
    14341 caggtgcgca tgtaaaacaa gcgcgagata gcccttcgtg tttttagcca tgtttttccc
    14401 tccgttgatg ttgtgcttga ccggtttatg gaatatcaac tcgggtcgtt ctggtcacaa
    14461 tgggtgtgat ctgtttctcc atgtcaccgt cagcgttgac ggcatggacg gggatgattt
    14521 gctctccgtc cggcagggcg aaacgaacac tgaaggtgcc gtccggtctc agcttgagtg
    14581 gctggccttg aagtgtgact ttggcgtccg gttctgttgc gccatagaca atcagctccg
    14641 tgtttacaac aagccaaaaa tccttgcctt tttcgccacc ttcgtctttc cgcggaacat
    14701 tggatgaact tgcgacagct cccgaggaaa ggttcgcggc aagatgctgg gccaagaggg
    14761 ctccgatgga ttcacttccg gcaagttcgg tgactttgta cccgccggag atccggtaca
    14821 tctctgtggc tgtttctgtc gttgtgtgcc actccggttt ctcgtcaatc cggtcagaaa
    14881 gtgtgtctct gggtgtcaat acggaatttg atctgg
//
LOCUS       DAOSHE010000005        14644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_3377_length_14644_cov_4.734320, whole genome shotgun sequence.
ACCESSION   DAOSHE010000005 DAOSHE010000000
VERSION     DAOSHE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14644
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3377_length_14644_cov_4.734320"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PLD82_00410"
     CDS             complement(<1..1017)
                     /locus_tag="PLD82_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63881.1"
                     /translation="MSLPHERFKAHGELSPLSTHLFSMTKILLVMLLVTISTRTVSSS
                     GSIATISRIETRQNSLVRSRLTSAADPAWPSSAPLHALWPDTITYFNKNLDLLHAARF
                     STNEFFTSDRWGYATYTKTGNTIEVFNTNGEKISTHNAPAWPRATGSSKWLLLYTGDQ
                     SGLAALERASGTLVAPYQQLASLVTATAINEQEQSIVFGMVDGSLEKFSLRDTKTIWR
                     QHPPEGRLSAIKGLTIQSGDSSILVVSGSRPETFSRYSNSGKLLWSTPTGGDLRTQVM
                     VHAGYRYTASQTAAGLILYDLDSGSIVRRLEPNLPSGKRVTWTSFQETKTGEILASIS
                     SGSFS"
     gene            1076..1936
                     /locus_tag="PLD82_00415"
     CDS             1076..1936
                     /locus_tag="PLD82_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOG63882.1"
                     /translation="MLRLVISASYTVGAMDEIKLALFQMSICPDKDDNLQKFEDWAAV
                     AVAEGADVLVLPELFTAPFDKDLLHAASDPFDGGETVSTVSDAAMRHGVFIVGGSFPE
                     QSDEGKMWNTAFAVSPDGEILAKYRKIHLFDPAYPGVDVRESDSMVPGDSLAKFLCCG
                     VLSSIAICYDLRFPALFAKMRKDGAGMVFVPAAFNHVSGPAHWELLVRARALDTQCFV
                     VACSPAPNPALSYSPWGHSMVVDPWGDVLLDAGSGEGIHYCTIRPERIQQIRTRIPLE
                     MHSRPELYGF"
     gene            complement(2008..2598)
                     /locus_tag="PLD82_00420"
     CDS             complement(2008..2598)
                     /locus_tag="PLD82_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63883.1"
                     /translation="MKKRLVLTGFTLLVCGSLIATGPDPFPPSSTVPNPGYNIPGLPL
                     PDGYARYKFGMTRDTVVEIIRTDPALKAWDADFIEGFEKEDWTVLSAVGPPHLDNAWF
                     VFDEKNELWCVTLNFNSSRYSYLEVAKVLKEKYGRPAILGQDVTIWQSDRVRIQLEKS
                     LHLKYIDLARFATVKREFSPELLKRETNRYGIFNSL"
     gene            2808..6311
                     /gene="nifJ"
                     /locus_tag="PLD82_00425"
     CDS             2808..6311
                     /gene="nifJ"
                     /locus_tag="PLD82_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOG63884.1"
                     /translation="MSKKFVTIDGNTAAAHVAYAFSEVAAIYPITPSSTMGELADEWA
                     AKHRINIFGQELSVVEMQSEAGAAGAVHGSLSAGSMTTTFTASQGLLLMIPNMYKIAG
                     EMLPTVFHVSARSLAAQSLSIFGDHQDVMAVRQTGFALMAANTVQETMDLALVSHLAT
                     LESEIPFLNFFDGFRTSHEIQKVEEIDYETMKSLIEHKFVERFRRRALNPEHPTLKVA
                     QQVADVYFQGRETTNRNYEDLPELIQKYMDKVGAAIGRSYKLFEYVGAPDADRVVIAM
                     GSACDAIEETLGYLQAKGEKVGLVKVRLYRPFSIKHLLAAIPASVKKIAVLDRTKEPG
                     SIGEPLYLDVKAALEGSGKTVIGGRYGLSSKEFTPAMIKAVYDHLNGKCFHNFTVGIN
                     DDVTHLSIPVGEDIDTEAADVKRCIFWGYGSDGTVGANKDAIKIIGDNTEMYAQGYFS
                     YDSKKSGGITVSHLRFGNSPIHSPYLINKADFVALHKPEYIGRYDILGPIKEGGTFLL
                     NSEWSNEEVFGHLTEDMQKIIIEKKINFYNINAYKIAHDVGLGNRINSVMQAAFFKIS
                     GVMPEAKAMELIKAAIKKTYGKKGDDIVKQNWECVDRTSEALVKVAVPSKPGKCAPVR
                     KLIPDNADAFAKEIIEPTMRLKGETIPVSKMAYDGVLPTATSRLEKRGVATEVPHWIP
                     ENCIQCNQCVFVCPHATIRAKQILPSDTAKAPSTFKTVKSKTKNERDLDYKIQVYVED
                     CVGCGSCVETCPKAALVMKPLAEERVAGETENAVYFDALPIITEGAPANTLKGSQFLQ
                     PLFEFSGACAGCGETPYVRLITQLFGDRMIVANATGCSSIYGGTFPTVPYCKNERGEG
                     PAWGNSLFEDNAEYGFGMRLAIDSNRKQLAALLADVKTASPELASAVDAMMSNWSKTD
                     EQAKAAADALKTALRSSLGKASGEVKSKIERAIALENYIVDKSVWIFGGDGWAYDIGY
                     GGLDHVLAMGRNVNVLVLDTEVYSNTGGQSSKSTPIGSIAKFAASGKKIGKKDLGLMA
                     MTYGYVYVASVNMGSDKAQLLKAILEAEAYEGPSLIIAYAPCINHGIDMRKSQEAEKK
                     AAASGHWPIYRFNPLLKAEGKSSLVWESKEPTLDFKEYLMSERRYAMLVKQDPAEAER
                     LFALAAEDAKRRFAQYKKFVD"
     gene            complement(6497..6913)
                     /locus_tag="PLD82_00430"
     CDS             complement(6497..6913)
                     /locus_tag="PLD82_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63885.1"
                     /translation="MPAKKSNTSMPPPRKKKEKKRDLYHWSDEDLSSLKKLYPTLANA
                     EIAKKLKRTKSSVDKKAHELSLRKERTYRSTIAAQNNAQRKNSWRADEVAKLKKLFDK
                     HTYAELASIFGRNAQSIQSKATRLGLWKYKTDSRST"
     gene            complement(7012..7494)
                     /locus_tag="PLD82_00435"
     CDS             complement(7012..7494)
                     /locus_tag="PLD82_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63886.1"
                     /translation="MKQAKAVKSPSKHTVKSTVSRKDLGAIKDEAAKKDRPRKFRGIW
                     TEQELDMLRNLYPLMTNVEVAKKLGRPKASVDKKGFDNGLKKAPEHRRKVAISNNKKR
                     RNSWTKEEEATLKKMYQKSTYRELAETLHRNPQSIQSKATKLGLWKYHTIRMRENGEV
                     "
     gene            7662..7961
                     /locus_tag="PLD82_00440"
     CDS             7662..7961
                     /locus_tag="PLD82_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63887.1"
                     /translation="MEKLIISLNAVELRILGIWAEAILHGGHWGDGAVILPDEEAAMQ
                     LLKSAQANSALACTPRNIFTFLVWSSASSETPEEQILREKLEKSLAGTGLRPPVL"
     gene            complement(8021..10933)
                     /locus_tag="PLD82_00445"
     CDS             complement(8021..10933)
                     /locus_tag="PLD82_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOG63888.1"
                     /translation="MGETVNQEEQTPSHVPACVAIGASAGGLEALEEFFKHMPATSGM
                     AFVVIQHLSPEYKSMMVELLSRHTKMKVCRVEDGMPLSPNCVHLIPPGFSMSIFHGKL
                     LLAPQISHTQGLNLPIDIFFHSLAEDQGERSIGIILSGTGSDGTLGVRAIKGAGGMVM
                     AQDEESAKFDGMPRSAIATGLVDFICPPSKMPDELMRYINHPFINQPETVEEKLLSDE
                     DTFGKILSIIRTRTGVDFSFYKPNTIIRRLERRISINRIDSIEEYVSCLHHYPNETSI
                     LFRDLLINVTRFFRDGEAWNYIIETVIPSLLEKTPQSMPLRIWSVGCSTGEEPYTLAM
                     LFQEHLMRTNTHREIKIFATDIDADALEVAGTGLYPENIAQDVSPERLKTFFIHTDSG
                     YRINESIRSMVVFAAHNIIKDPPFSRIDLIVCRNLLIYLKPAMQQKIINSFRFSLPAG
                     GTLFLGSSETAGDNNTALVPVNQKWKIYQHRTETSSRRPSTIDFETTHSGTIAQVTRS
                     GLTRREPSLDELITTSLLDSFIPPGVVISESGDIQRVCGDIREFVHLPPGKPTSSLLR
                     MVNPELGIAITTGIHRAIKDNEVVRFRNFKFTTGNDTERIIDLSVRKLGAGIFPQQFL
                     LITFEQIAQSGKDTRAVEASMPHNERILELEQELQYNRENLQATIEELETSNEELQAT
                     NEELIASNEELQSTNEELQSVNEELYTVNSEYQQKIEELTQLNNDMDNLLKSTEIGTI
                     FLDQMQHVRKFTPAATRYINLTQADIDRPISHFTSTIQNCQFVELAGEVLRDLVPRQA
                     EGVLANGGIVLIRMTPYRTRQNAIDGVVITFIDISERKRFEQDIAHERDVLLTILENS
                     PVGKTMVDANGRITFVNKRASEILGITHEEFKSRTWDDVRWEILAEDNTPLSPEMLPY
                     SIIRRTRKPAYNLRHNIKTGDGRTITLTINAAPIIKQDGAFDGAVFSLLEVVPETL"
     gene            complement(11166..11999)
                     /locus_tag="PLD82_00450"
     CDS             complement(11166..11999)
                     /locus_tag="PLD82_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63889.1"
                     /translation="MKQIGIIAVLLLVVLTGAVQAEDSKIGLDLGLEYDSFYAFRGGP
                     NFTDRVAAGAILPSLGYAAGDFYFLVMGEIAPSILGINTMADNGSYGDGASAQEKAWT
                     GLDVGAMWSKSVADEALTIGAEVWFLLYPGSKDQLGYDATYFTGAISITLSSLPLSPK
                     IMFTQAFKPDDANGTRNKMEDRYIALSFGDSVDLVKDVSKLDLGMNIGFWMDETSKGS
                     KYSALSDVVGSAKYSVSVASGVTLWSSFHMGYVPQSDWALGKDGGDFRFWLKFGISYT
                     L"
     gene            12171..12569
                     /locus_tag="PLD82_00455"
     CDS             12171..12569
                     /locus_tag="PLD82_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HOG63890.1"
                     /translation="MRRVVIIGLDAGRWQAVRAAFAGYEDAPEFVAAGQDQRDWLVKD
                     LVAGEAGADAAASLPEAGGSVIVFFAGFDGRESSRCIDQTRGLFGPDVIFAGLTTTNA
                     GWPLARLVAELVAERAAFKKRSATVGPEEG"
     gene            complement(12710..13924)
                     /locus_tag="PLD82_00460"
     CDS             complement(12710..13924)
                     /locus_tag="PLD82_00460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG63891.1"
                     /translation="MKPIKKASVLKNVCYDIRGPALQEAARLEADGVKILRLNIGNTA
                     PFGLDTPDELIQDLIKNVRSAQGYSDSKGIFSARKAVVQEWQQRGMDISIEDVYMGNG
                     VSELIKVAVEALLNNGDEVLVPAPDYPLWTASVNLARGKPVHYLCDEKSGWLPDMADL
                     KKKVGPKTKAIVIINPNNPTGAVYPKETIEGLVRFAEEHNLIIMSDEIYDKILYDNMA
                     HYSPACCTDKTLVITFSGLSKVYRAPGFRCGWMVISGAKEAARDYIEGINMLTSMRLC
                     ANVLAQNTVQTALGGRQSIYDLTRPGGRLERQRTLAWEMLNQIPGVSCVKPTGALYMF
                     PKLDKKMYPFKSDMDFLLGLLREEHVLVVQGTGFNWPDTDHFRLVFLPEEEQLKQAVE
                     GIGRYLGSVARI"
     gene            complement(14023..>14644)
                     /locus_tag="PLD82_00465"
     CDS             complement(14023..>14644)
                     /locus_tag="PLD82_00465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63892.1"
                     /translation="ADSRSAALEKSKAVTFHESFEADTRILACIIDKLFEDLERAGYS
                     EEEGQEIVVAVDEAITNAVLETIKATGEVLDNFAVALKKGPLKMIAVKWEITDQDFTA
                     TVIDHGAGLDLDEVQQRTPQTGTGDYLNQVDDYQHKCNLRMRVNGESVELKRLGAGLK
                     IMATFMDDIFIDLIDSCEIISSTVSHCTTGTILTLHRNRKHKAVSA"
BASE COUNT         3101 a         3632 c         4141 g         3770 t
ORIGIN      
        1 actgaaactg cccgaagaga tcgaagcgag gatctcaccg gtttttgttt cctgaaacga
       61 agtccatgtg actctttttc ctgacggaag attcggttca agtctgcgga cgatgctccc
      121 ggaatccaga tcatagagaa tcaggcccgc tgcagtctgg cttgcggtgt atcggtatcc
      181 ggcgtggacc atgacttgtg tccggagatc gccgccggtg ggagtgctcc aaagcaattt
      241 tccagaattg gagtatcgtg aaaatgtttc aggtcttgag cctgagacga ccagtatgga
      301 tgaatcgccg gattgtattg tgagtccctt gatagctgag agtcttcctt caggcggatg
      361 ctgtcgccag atggtctttg tatcgcgcaa ggagaatttt tcaaggcttc cgtccaccat
      421 gccgaacacg atggattgtt cttgttcgtt tattgctgtg gccgttacga gtgacgccag
      481 ctgttgatag ggggccacga gggttccgct tgctctctcg agtgcagcca gtccggattg
      541 gtcgcccgtg tacagaagaa gccattttga tgagcccgtg gctcttggcc aagccggggc
      601 attgtgcgta gaaatttttt ctccgttggt gttgaaaact tctatcgtgt tgcctgtttt
      661 tgtgtacgtg gcgtaacccc agcggtcact ggtaaagaat tcatttgtcg agaatcttgc
      721 tgcatgaagc aggtcgagat ttttgttgaa atatgttatt gtatctggcc acagtgcgtg
      781 caacggagcc gatgaaggcc aagcagggtc tgcggcggaa gtcagtcgcg agcgtacaag
      841 cgagttctgc cttgtctcaa tgcgcgagat tgtggcaatg gatccgctgc ttgaaactgt
      901 tcttgtcgag atggtaacaa gaagcatgac aagaagaatt tttgtcatgc tgaagagatg
      961 cgtggagagg ggggaaagtt ctccatgtgc tttgaatcgc tcatgcggaa gggacatgcc
     1021 agacctcttg ctctggtttt tatcaaatat agcaaataac cgggagtgac attttttgtt
     1081 gcgcctcgtc atcagcgcaa gctatactgt gggcgccatg gatgaaatca aacttgccct
     1141 ttttcagatg tccatatgtc ccgacaaaga tgacaatctt caaaaatttg aagattgggc
     1201 cgctgtcgct gtggccgaag gagccgatgt tctggttttg ccggaactct ttacagcgcc
     1261 attcgataaa gatcttcttc atgctgccag tgatccgttc gatggtggtg agacagtcag
     1321 tacggtttct gatgcagcca tgaggcacgg tgtattcatt gttggtggat catttcccga
     1381 gcaatcggat gaagggaaga tgtggaatac tgcctttgcg gtgagtccgg acggggagat
     1441 cttggccaag taccggaaga tccacctttt tgatcccgcc tatcccggtg ttgatgtccg
     1501 tgaatcggac agcatggtcc ccggtgacag tttggcaaaa tttttgtgtt gtggcgtcct
     1561 ctcctcaatt gctatttgct acgatttgcg ttttccggca ctcttcgcaa aaatgagaaa
     1621 agatggcgca ggcatggttt ttgttcctgc ggcattcaat catgtatcgg gtccggctca
     1681 ctgggagctt ctcgtccggg cgcgagcgct ggatacccaa tgttttgttg tcgcctgcag
     1741 tccggcaccc aatcctgccc tctcgtacag tccatggggt cattccatgg tggtcgatcc
     1801 gtggggtgac gtcttgctgg atgcgggaag tggagaaggg atccattatt gcacgatccg
     1861 gccggagaga atccagcaga tccggacccg gatcccgctg gaaatgcatt ctcggccgga
     1921 attatacgga ttttgagacc ggtatgcgca cccggatggg gtttgggctg ttttttcgca
     1981 caagtcacgg ctgttatggt gacgctttca gagcgaattg aatattccgt aacgatttgt
     2041 ttctcttttc aggagttccg gcgagaattc ccgcttgacg gttgcaaacc gggcgagatc
     2101 gatgtatttc agatgcaggc tcttctcaag ctgtattctc acccggtcgc tttgccaaat
     2161 agtcacatcc tggccaagaa tggccgggcg gccatatttt tcctttaaaa ccttcgcgac
     2221 ttcgagatag ctgtatcggc tcgaattgaa attcagggtg acacaccaga gttcgttttt
     2281 ttcatcaaaa acaaaccagg cgttgtcaag atggggcggt ccgactgcgg aaagaacggt
     2341 ccagtcttcc ttttcaaaac cttcgatgaa atcggcatcc caagctttca gtgccgggtc
     2401 tgtccggatg atttcaacga cggtgtcgcg ggtcattccg aacttgtagc gcgcatagcc
     2461 gtccggcaag gggagtcccg gaatgttgta tcccgggttg ggcactgtgc ttgatggtgg
     2521 gaaagggtcc gggcctgtcg cgatcaagga gccacacacg aggagggtga acccggtcaa
     2581 aacgagcctc ttcttcacga ctcattgctc cttctgggct aaagactgtt caccatgatt
     2641 atcggcacag ttcttgtatc ttccagtata tttaccacgg cccgacctgt cttgcatgac
     2701 gaacgtgatg ttggtctgcg agtatgggga aagccgctgt tgtgccgccc acacacaggc
     2761 tgcacagcgg aatcaggttc accaaaccct tacaaggagc tgttataatg agcaaaaaat
     2821 tcgtgaccat tgacggcaat acggctgctg cccatgtagc ctacgccttc agcgaagtgg
     2881 ccgctatcta ccccatcacg ccttcttcaa cgatgggtga gttggctgat gaatgggcag
     2941 ccaaacaccg tatcaacatc tttggccagg aattgtcggt tgttgaaatg cagtcggaag
     3001 ccggtgcagc cggcgcggta catggatctc tttccgccgg ctcgatgacg acaaccttta
     3061 cggcttccca gggattgctg ttgatgattc ccaacatgta caagattgcc ggcgaaatgc
     3121 ttccgaccgt ttttcatgtt tctgcccgca gccttgccgc ccagtcgctc tccattttcg
     3181 gggatcatca ggatgtcatg gcggtccgtc agaccggttt cgccctgatg gcagccaata
     3241 cggtccagga gacaatggat ttggcgctcg tctcgcatct cgccacgctt gagtccgaga
     3301 ttcccttcct gaatttcttt gacgggttcc gcacctccca tgaaatccag aaagttgaag
     3361 agatcgacta tgagacgatg aagtctctca tagagcacaa gtttgtcgaa cgtttccgcc
     3421 gccgtgcact caacccggag cacccgacgc tcaaggtcgc gcagcaggtt gctgatgtct
     3481 acttccaggg ccgtgagacg accaacagga attacgaaga ccttcctgag ttgattcaga
     3541 agtatatgga taaagtcggt gccgccatcg gtcgttccta taaactcttc gagtatgtcg
     3601 gagcaccgga tgcagacagg gtcgtgattg ccatgggttc tgcctgcgat gccattgaag
     3661 aaacccttgg atatctccag gcaaagggtg agaaagtcgg tcttgtcaag gttcgcctct
     3721 acagaccgtt ctccatcaag catctcttgg cggccatacc ggcgagtgtc aagaagattg
     3781 ccgttcttga caggaccaag gagccgggtt ccatcggaga gccgctctat cttgatgtca
     3841 aagcggcgct tgagggctcc gggaagacgg tcatcggtgg acggtatgga ttgtcctcca
     3901 aagagtttac accggccatg atcaaggctg tctacgatca cctcaatgga aagtgtttcc
     3961 acaactttac tgttggcatc aacgatgacg tgacccatct ctcgattccg gtcggcgagg
     4021 atatcgatac cgaggcagcg gatgtcaagc gctgcatctt ttggggatat ggctcggatg
     4081 gtacggttgg tgccaacaag gatgccatca agatcatcgg tgacaacacg gaaatgtacg
     4141 cacaggggta tttctcctat gattccaaga aatcaggggg catcaccgtc agccatcttc
     4201 gcttcggcaa ttccccgatc cattcaccct atttgatcaa caaggccgat tttgtagccc
     4261 tgcacaagcc cgagtatatc ggtcgctatg atatccttgg gccgatcaag gagggcggta
     4321 ccttccttct gaactcggag tggtcgaacg aagaggtttt cgggcacctg accgaggata
     4381 tgcagaagat catcatcgag aagaagatca atttctacaa tatcaatgcc tacaagattg
     4441 cccatgatgt cggactcgga aaccgcatca attcggtcat gcaggcggca tttttcaaga
     4501 tctccggtgt catgcccgaa gccaaagcga tggagctgat caaggctgcc atcaaaaaga
     4561 cctatggcaa aaagggcgat gatattgtca agcagaactg ggagtgtgtc gaccggactt
     4621 cggaggccct tgtcaaggtc gctgttcctt ccaagcccgg caagtgtgct ccggtccgca
     4681 aacttatacc cgacaatgcg gatgcatttg ccaaagagat catcgagccg accatgcgtc
     4741 tcaagggcga gacgatcccg gtctccaaga tggcctatga cggtgttctc ccgacagcta
     4801 ccagccggtt ggagaaacgt ggtgtggcca ctgaagtacc tcactggatt ccggaaaatt
     4861 gtattcaatg caatcagtgc gtttttgtct gcccgcatgc gacgatccgg gccaagcaga
     4921 ttctgccttc ggatactgcg aaggcgcctt ccacgttcaa gacggtcaag tccaagacca
     4981 aaaacgaacg cgatctggat tacaagatcc aagtttacgt tgaggactgt gtcgggtgtg
     5041 gaagctgtgt cgagacctgt cccaaggctg ccctcgtcat gaaaccgttg gcagaagaac
     5101 gggtagctgg tgagacagag aatgcagtct attttgatgc tctcccgatc atcaccgaag
     5161 gtgctcctgc caatacgttg aagggcagcc agttcctgca gcccctcttt gagttctccg
     5221 gagcctgcgc aggatgtgga gagacccctt atgtccgcct cattacccag ctttttggcg
     5281 atcgcatgat tgttgccaat gcgaccggtt gttcttcgat ctatggtgga acattcccca
     5341 ctgttccgta ttgcaagaac gagcggggtg agggccctgc atggggcaac tccctctttg
     5401 aagacaatgc tgagtatggt tttggaatgc gcttggctat tgactcgaac cggaaacagc
     5461 ttgctgccct gctcgcggat gtcaagacgg cttcccctga acttgcttcg gccgttgatg
     5521 ccatgatgtc gaactggtca aaaaccgacg agcaggccaa ggccgctgcg gatgccctca
     5581 agactgctct caggagttca cttggaaagg catcgggcga agtcaagagc aagatcgaac
     5641 gggcaatcgc tctcgagaat tacatcgtcg acaagagcgt ctggattttt ggcggtgatg
     5701 gctgggccta cgacatcggc tacggcggac tcgaccatgt tctcgcaatg ggccgtaatg
     5761 tgaatgttct cgttcttgat acagaggtct actcgaacac cggtggtcag tcttccaagt
     5821 ccactccgat cggctccatc gcgaagtttg cggcctccgg aaagaaaatc gggaaaaagg
     5881 acctcgggct gatggcgatg acctacggct atgtctatgt ggcttctgtg aacatgggct
     5941 ccgataaagc tcagcttttg aaggccattc ttgaggcgga ggcctatgaa ggaccatcct
     6001 tgattattgc gtacgcaccc tgtatcaacc atggcatcga catgcgcaag agccaggaag
     6061 ccgagaaaaa ggcggcggct tcggggcatt ggccgatcta tcgcttcaat cccctcctca
     6121 aggccgaggg aaaatcctca ctggtctggg aatcgaaaga accgacgctt gatttcaagg
     6181 aatatctcat gtccgagcga cggtatgcga tgcttgtcaa acaggatccg gccgaggccg
     6241 agagattgtt tgcgctggct gccgaggatg cgaaaagacg gttcgcccaa tacaagaaat
     6301 tcgttgactg acctgatcgc cgggatgcgc tcttgctgac agtatcccgg cagtgacgat
     6361 tcatcgagga agggcccctg ccgggacggt tgttctggcg gggggttttt tttgaaacaa
     6421 atggtttttt gagtacaaaa gaaagccgcc aatggattgc gttggcggca tgtgcgggct
     6481 gagagggtgc tgcgggtcag gtggagcggc tgtccgtctt gtatttccag agaccgagac
     6541 gggtggcttt gctctggata ctctgggcgt tgcgcccgaa aatcgaagcc aattcggcat
     6601 aggtgtgctt gtcaaagagt tttttgagct tggcgacctc atccgcgcgc caggagtttt
     6661 tccgctgggc gttgttttgt gcggcgattg tgctccgata ggttctttcc ttgcgcaggg
     6721 acagttcatg cgcctttttg tccacgctgg attttgttct tttgagtttt ttcgcgatct
     6781 cggcattggc cagtgtcgga tagagttttt tcaggctgga aagatcctcg tcagaccagt
     6841 gatacagatc gcgttttttc tccttttttt ttcggggcgg gggcatcgaa gtattcgatt
     6901 tttttgcggg catgacatcc tccagaaaaa cagtagttgt aatgtactat tatgaacaac
     6961 acaagaggcc gatatccctt gatcggcctc ttgtgcagct cttgagcggt tttacacctc
     7021 tccgttctcg cgcattctga ttgtgtggta tttccacaat ccgagttttg tcgccttgct
     7081 ttgaatgctc tgcgggttgc gatgcagggt ctcggcaagt tcacgatagg tgctcttctg
     7141 gtacatcttt ttgagcgtcg cttcctcttc ttttgtccag gaattccggc gttttttgtt
     7201 attggaaata gccactttcc ggcggtgttc gggggccttt ttcagtccgt tgtcgaaccc
     7261 ttttttgtca acacttgcct tgggccggcc caattttttt gcgacttcaa cattggtcat
     7321 cagcggatag aggttgcgga gcatatccag ctcctgctcg gtccagattc cccggaattt
     7381 gcgggggcgg tccttctttg ctgcttcgtc cttgatggcc cccaagtctt tcctggatac
     7441 cgtcgactta accgtatgtt tggacgggct ttttactgct tttgcttgct tcatctccaa
     7501 tctcctttgg tactgaattt gggttgcagg aaacccaata tatatccctt ttccaaatag
     7561 tatctcaaac cgaagaaaaa atcaagaaat attgcaaaaa acatcacaaa agcgtgtttt
     7621 tcaagcttga tccgaagatt ttgcataagt agccttcatg aatggaaaag ctcataatca
     7681 gtttgaacgc tgtcgagctg cgcattctcg gcatctgggc agaggcgatt ttgcatggag
     7741 ggcattgggg tgacggtgcc gtcatccttc cggatgagga ggccgccatg cagttgttga
     7801 aatctgctca agcgaatagt gcgttggcgt gcacgccgag gaacatattt acatttcttg
     7861 tctggtcctc cgcaagcagt gaaactcccg aagaacagat tcttcgcgaa aagctcgaaa
     7921 aatcacttgc cggcaccggt ttgcgtcccc ccgttctctg acttctcacc aaaagtggcg
     7981 tgaggctgtt caccagaaaa aagagccggg atcacagacg ttacagggtc tctggcacga
     8041 cttcaagcag gctgaatacg gcaccatcga atgccccgtc ctgtttgatg atgggagctg
     8101 cgttaatggt cagcgtgata gtcctgccgt ctccggtttt gatgttatgc cgaaggttgt
     8161 atgccggttt ccgggtgcgt ctgataatgc tgtacgggag catttcagga gacaatggcg
     8221 tgttatcctc ggcaaggatt tcccagcgga catcatccca tgtcctgctt ttgaattctt
     8281 cgtgcgttat tccaaggatc tcactggccc gcttgtttac aaaggtgatc cgtccgtttg
     8341 cgtcaaccat tgttttcccg acagggctgt tctcaagaat cgtcaacagc acgtcacgtt
     8401 catgcgcgat gtcctgttcg aagcgttttc gttcggaaat atcgataaag gtgatcacga
     8461 cgccatcaat cgcattctgt ctggtccggt agggtgtcat cctgataaga acaatgccgc
     8521 cattggctaa aacaccttcg gcctgtctgg ggaccaggtc tctgagcact tcgccggcaa
     8581 gttcgacaaa ctggcagttt tgtatggtgc tggtaaaatg actgatcggg cggtcgatgt
     8641 ccgcctgtgt cagattgata tatctggttg cggcaggagt gaacttgcgg acatgttgca
     8701 tctggtccaa aaaaatggta cctatctcgg tgctcttgag gaggttgtcc atgtcgttgt
     8761 tcaactgggt cagttcttca attttttgct gatattcgga attgacggta tagagttctt
     8821 cgttcactga ttgcagttct tcgttggtgc tttgcaattc ttcgttggaa gcgatcagtt
     8881 cttcattggt cgcctgcaat tcctcattgg atgtttcaag ctcttcgatg gttgcctgca
     8941 ggttttccct gttgtactga agttcctgtt ccaattcgag gatccgctcg ttatgcggca
     9001 tgctggcttc gacggctcgt gtgtcttttc ccgattgagc aatctgttca aaagttatga
     9061 gtaaaaactg ctggggaaat attccggctc ccagcttgcg cacactcaga tcaatgatcc
     9121 gttcagtatc gtttcccgtg gtaaacttga agttccggaa acggacgacc tcgttgtcct
     9181 tgatggctct gtgtatcccg gtcgtaatcg caatcccgag ctctgggttg accattcgca
     9241 acagggagga tgtcggtttt ccaggtggga gatggacgaa ttcccggata tccccacaga
     9301 ctcgctgtat gtcgccactc tccgagataa cgacgccagg gggaatgaag ctgtccagca
     9361 ggctcgttgt gatcagttca tcgagggaag gttcacgcct ggtcagccct gaacgagtca
     9421 cttgcgcgat cgttccgcta tgtgtggttt cgaaatcgat ggtacttggt cgtcggctgc
     9481 tggtctcggt tctatgttga tagatcttcc atttttggtt gactggtacc agcgcggtat
     9541 tgttgtctcc tgcagtctca ctcgatccga gaaacagggt tccgcccgcc ggaagggaga
     9601 agcggaagct attgatgatc ttctgttgca ttgccggctt gaggtagatc aggagattgc
     9661 ggcagacgat cagatcgatc ctggaaaaag gcgggtcctt gatgatatta tgtgccgcga
     9721 aaacgaccat acttcggatt gattcattaa tcctgtagcc ggaatctgta tgaatgaaaa
     9781 aggtcttgag tcgctccggg ctgacgtcct gtgcgatgtt ttcagggtag aggccggttc
     9841 cggcgacttc aagagcatct gcatcgatat cagttgcaaa aatcttgatc tcgcggtgcg
     9901 tatttgtccg catcaggtgt tcctggaaga gcatggccag cgtgtacggt tcttctccgg
     9961 ttgaacaccc gacactccag atgcggagcg gcatactctg cggggttttt tccaggagcg
    10021 acgggatgac tgtttcgata atatagttcc atgcttcgcc atcgcggaag aatcgtgtta
    10081 cgttgatgag caggtcccgg aaaagaatgg aagtttcatt ggggtaatgg tgcagacagg
    10141 agacgtattc ttcaatggaa tcaatgcggt tgatgctgat ccggcgttcc agtctgcgga
    10201 tgattgtatt tggtttgtag aaggaaaagt cgacgccggt tcgtgtgcgg atgatcgaca
    10261 agatttttcc aaaggtgtct tcgtcgctca gcagtttttc ttcgacggtt tcgggctggt
    10321 tgatgaaggg atgattgatg tagcgcatca actcgtccgg catttttgac ggtgggcaga
    10381 tgaaatcaac cagtccggtt gcaatggcgc tccggggcat tccgtcaaat ttggcggatt
    10441 cttcatcttg agccataacc attccgccag cacccttgat ggcccggaca ccaagggtgc
    10501 cgtcgcttcc tgtccctgag agaatgatac caatggatcg ctcaccctgg tcttcggcaa
    10561 gagagtggaa aaagatgtca atgggaagat tgagtccctg tgtatggctg atttgtggag
    10621 cgagaaggag ttttccgtgg aaaatcgaca tggaaaaccc gggagggatg agatggacac
    10681 aatttggcga gagcggcatc ccgtcttcaa cccggcagac tttcattttg gtgtgtcgtg
    10741 ataaaagctc gaccatcatg cttttgtatt ctggcgaaag atgctggatg acaacgaagg
    10801 ccatgcctga tgttgccggc atgtgtttga aaaattcctc gagtgcttca aggcctcccg
    10861 ccgatgcacc aatcgccaca caggcgggaa cgtgagatgg cgtctgctct tcctgattga
    10921 ctgtttctcc catggtatct ctcccgcgaa tttcgtccct caacgagagt ataggcacga
    10981 aacaggtata tggaaagtct catagtggac aggaagcgga tattgagacg aataccggta
    11041 tcagagtccg tgggaaaagc gggagaacag gtacaattcg gagaatgtca ccatgatgac
    11101 ggtatggatg catcaaaaaa gcaccacctg gaccggtggt gcttttctaa acaatctgtt
    11161 tcagatcaga gggtgtaact gatgccgaat ttgagccaga agcggaaatc tccaccatct
    11221 ttgcccagtg cccagtcgga ctgggggaca tagcccatgt ggaacgagct ccagagggtg
    11281 acgccgctgg caacgctgac actgtacttg gcgcttccaa cgacatcgct cagcgcggag
    11341 tacttgcttc ctttactcgt ttcgtccatc cagaatccga tattcatacc gagatcaagt
    11401 ttcgaaacat ccttgaccaa gtcaacgctg tccccgaagg acagggcgat gtagcgatcc
    11461 tccattttat ttcgggttcc gttggcatca tccggtttga aggcctgggt gaacatgatt
    11521 ttcggagaaa ggggcaggct ggagagggta atgctgatgg cgccggtaaa ataggtggca
    11581 tcatagccga gctgatcttt ggagcccgga tacaggagga accagacttc ggcgccgatg
    11641 gtcaaagctt catcggcgac gctcttgctc cacatcgcgc cgacatccag ccctgtccag
    11701 gctttttcct gggcactggc gccgtcacca tagctgccgt tatccgccat ggtgttgatt
    11761 ccgaggatcg aaggggcaat ctctcccatg acgaggaaat aaaaatcacc ggctgcataa
    11821 cccagcgagg gaaggatggc tccagccgcg acacgatcgg tgaaattggg accgccgcgg
    11881 aaggcgtaaa agctgtcata ctcaaggcca aggtcgagac cgatcttgct gtcttcggcc
    11941 tgcactgcgc ctgttaagac aacgagaagg agtactgcaa tgataccaat ctgtttcatg
    12001 atcttctcct gtttatgtag agtggtacca tcaggatacg tcctcgagcg gtccgcgtca
    12061 aggagaagtc ccatgacgaa attcggaacc tgctggaatg gtttcgaagg gggcgtcgtt
    12121 gccccgtccg agtccgggtg ctgatattta ggaagaacag gagggggctg atgcgcaggg
    12181 tggttattat tgggcttgat gccggaagat ggcaggctgt tcgggcggcc tttgccggat
    12241 atgaggatgc gcccgaattt gtcgctgccg gccaggatca gcgggactgg ctggtcaagg
    12301 atctcgttgc gggtgaagcc ggagcggatg cagcagcgag tttgcccgag gccggtggaa
    12361 gcgtgatcgt gttttttgcg ggatttgacg gccgggagag ctcgcgctgt atcgaccaga
    12421 cacgggggct ttttgggccg gatgtgattt ttgcgggcct gacgacaacc aatgcgggct
    12481 ggccgcttgc ccggttggtc gccgagcttg tcgcggagcg cgcggcgttc aagaaacgtt
    12541 cggcgacggt tgggcccgaa gagggctgaa cgagccatgc ctcgtttctg atgtgtcttc
    12601 cggggcgtga ctctccgtat tcgcatccat gccgggtggc cgagtcagcg atgttccccg
    12661 gctcgctccg ccaatacccg gcaagaggtt gttcctcaaa aaatggctct tagatcctgg
    12721 caacacttcc cagataccgg ccgattcctt cgacggcctg cttgagctgt tcttcttcgg
    12781 ggagaaagac caaccggaaa tggtccgtgt ccggccagtt gaacccggtt ccctggacaa
    12841 cgaggacatg ttcctcccgg agcagtccga gcagaaaatc catgtcgctc ttgaaggggt
    12901 acattttttt gtccagtttg gggaacatgt agagggctcc ggtcggtttg acgcaggaaa
    12961 ctcccggtat ttggttgagc atttcccatg ccagggtgcg ctggcgctcg agccgcccgc
    13021 cgggcctggt caaatcgtag atgctctggc ggccaccgag tgcggtctgg acggtatttt
    13081 gggccaggac attggcgcag agccgcatcg atgtcagcat gttgatcccc tcgatgtaat
    13141 cccgggcggc ttctttcgct ccactgatga ccatccagcc gcagcggaat cctggagcgc
    13201 ggtagacctt ggagaggccg ctgaaggtga tcacgagggt cttgtcggta cagcaggccg
    13261 gtgagtagtg cgccatattg tcgtagagga tcttgtcgta gatctcgtcg ctcatgatga
    13321 tcaggttgtg ttcttccgca aagcgcacga gtccctcgat ggtctctttg gggtagaccg
    13381 cgccagtcgg gttgttcggg ttgatgatca cgatggcttt ggtcttcggt ccgacctttt
    13441 ttttcaggtc agccatgtcc gggagccagc ctgatttttc gtcacagagg taatgcacgg
    13501 gcttgccacg cgccagattg acggaggctg tccagagggg gtagtcgggg gcggggacaa
    13561 ggacttcgtc tccgttgttc aacagggctt cgacggcaac cttgatcagc tccgagacgc
    13621 catttcccat gtagacatcc tcgatggaga tgtccattcc ccgctgctgc cactcctgta
    13681 ctacggcctt tcgcgccgag aagatcccct tggagtcgct atagccctgc gcgctgcgca
    13741 cgttcttgat caggtcctgg atcagttcat ccggtgtgtc cagtccgaag ggtgcggtgt
    13801 ttccgatgtt caaacggagg atcttgacgc cgtccgcttc cagccgcgct gcttcctgaa
    13861 gggctggtcc tcggatgtcg taacacacgt ttttcagtac gctggctttt ttgattggct
    13921 tcatggcagt cctcacttcc tcgtcccgga gggcgcttcc cgggcgggta tcccaaaata
    13981 tgctatgatc cggagggaaa aggaatggtc tgcggggaaa aatcaggcgc tcacggcctt
    14041 gtgcttgcgg tttcgatgca gggtgagaat cgtcccggtt gtgcagtgac tgactgtgga
    14101 agagatgatt tcgcaggaat cgatcaggtc gatgaagatg tcgtccatga aggttgccat
    14161 gatcttgaga ccggctccga gacgcttgag ttcgacactc tctccgttga cgcgcatccg
    14221 gaggttgcac ttgtgctggt agtcgtcgac ctgattgagg tagtcaccgg tccctgtctg
    14281 cggggtccgc tgttggactt cgtcaagatc cagtccggct ccatggtcga tgaccgtggc
    14341 ggtgaagtcc tggtcggtga tctcccattt gactgcgatc atcttgaggg gccctttttt
    14401 aagagccacg gcgaaattgt cgagaacctc gccggttgcc ttgatggtct cgaggactgc
    14461 gttggtgatc gcttcatcga cggcgacaac aatctcctgc ccttcctcct cggagtatcc
    14521 ggcccgttca aggtcttcaa agagcttgtc aataatgcag gccagaatcc tggtgtcggc
    14581 ctcgaagctc tcatggaagg tgacggcttt gcttttttca agggccgcgc tccggctgtc
    14641 ggca
//
LOCUS       DAOSHE010000006        14465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_3445_length_14465_cov_4.862595, whole genome shotgun sequence.
ACCESSION   DAOSHE010000006 DAOSHE010000000
VERSION     DAOSHE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3445_length_14465_cov_4.862595"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLD82_00470"
     CDS             complement(<1..478)
                     /locus_tag="PLD82_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOG63893.1"
                     /translation="MAINDLFSQEVKVVNTGMTAFRDDLAHCGVSVIQVDWKPPVGVS
                     PESLDIIDDNLAKIDEANAKALEIILKGKPVLTGMGVALDTIPGMKKNLILHSGPPIT
                     WERMSGPTRGAVIGALIYEGMAKTPEEAEALAASGKIEYAPCHEHDAVGPMAGIVSA"
     gene            complement(482..2041)
                     /gene="fdrA"
                     /locus_tag="PLD82_00475"
     CDS             complement(482..2041)
                     /gene="fdrA"
                     /locus_tag="PLD82_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOG63894.1"
                     /translation="MVLKGFVKKGEYFDSVSLMIVSKEVNALPGIADSAIVMGTAQNK
                     EIVRGANLYIPELFDQADDTDLLLALKADSDKVVEETLVKIDDLFKALRNKKDEGGSN
                     FSPKSLEGAVEALPGANISLISVAGRYAGREAMRALKQGLHVMMFSDNVSIEDEIKLK
                     RFAHDKGLLVMGPDCGTAVINGVPLAFANVLSKGDCGIVAASGTGLQEVMTIISNEGA
                     GISQAIGTGGRDVKEAIGGIMFIDAMRALAADPATKVIVLVSKPPHESVLRKIADEIK
                     TIKKPVVGIFIGGDPKILEAAGAIAGSTLEETAVIAAALSKGASVDDCKKKIADRKAG
                     IIEQAKALAPKCRGKYLRGLFSGGTLCDETQLICKDMIGYINSNAPLNPEYRLKDSWK
                     SVGHTIIDLGEDEFTVGRPHPMIDFSLRNKKITDEAADKDVAILLLDLVLGYGSHMDP
                     LSEFVPAFTEAKKIAPELLIICSITGTDGDPQNRSRIKAGLERAGAIMMPSNAAASEL
                     AGNIIRILEGK"
     gene            complement(2031..2990)
                     /locus_tag="PLD82_00480"
     CDS             complement(2031..2990)
                     /locus_tag="PLD82_00480"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOG63895.1"
                     /translation="MKGLSPYPCPRRCRISSPRVLGIGASVLPGEYRVYARFHAFIDI
                     LTEAGIVGITDQADQAGPSTIILSPDLPSDWVSTVQSVSITETALSLDSLVLPIEGAQ
                     RYRETLQLPELSMPIDLQPALMLFQKILLSEAPEKSMAVVLDHSRTTAFSGNFDRIML
                     QHFLDGAEACWREEYGQAVASLLGSGYGLTPSGDDFLCGVLFGMHFANATNRKVCPIH
                     QLSTHLRAALPTCSVFSRTFLDHAIAGRWPLALRDAAEAILSVDTTTPDGQTRLETLF
                     RRVLAHGSTSGSDLASGFILTAGRYSGFSPSFVSWHKERNNGA"
     gene            3231..3506
                     /locus_tag="PLD82_00485"
     CDS             3231..3506
                     /locus_tag="PLD82_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63896.1"
                     /translation="MSPLHGSFDSMFSPARLGELSESSRQIGNDLDSLNSRLDFAGSN
                     TKQLAIMFRTLFQAAASRGLISDTEFRDRLDSVDLGEWTQSGENSGQ"
     gene            3560..3823
                     /locus_tag="PLD82_00490"
     CDS             3560..3823
                     /locus_tag="PLD82_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63897.1"
                     /translation="MPFSDQLFSNLFPAAKMRELEDLNRKEYEDVKNDIDSIKQRLED
                     LEVCNKQLGIMFRTLYDLAIEKNMITREEFRDLLDKVDLSEWV"
     gene            complement(3950..5059)
                     /locus_tag="PLD82_00495"
     CDS             complement(3950..5059)
                     /locus_tag="PLD82_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63898.1"
                     /translation="MESSLTLQTLLDALSSCKRQHIIIRGSPDPDALASAWALALLGE
                     TAGVPSLVYIEQPPSLSQNRALVRVLGLRLHMLETLKRAVRKGDGYCIVDYPDARLET
                     LPEWVPCSLHIDHHTPFPNQPVAKVRSINTACGAASTIIAVAVFDENARFQHDKKRLL
                     ASALLAGILTDTDQMRLAGDMDRAALGLLSHHADDRLVRRISLPTLSSEILHEQEEAE
                     KHAVSYKGWLITGLGYIDATSRDTIAIVADRLLEAHREAEAVVVFAGIEDPARGTLVL
                     DASLRSRDGDLDLERIIKSITHHGGARRHKGAFQIPLEFLRYATDRTRLWSVLEDAAI
                     HALMMACDKHGRKGILNAIFAPIRRFFGRLRGRNR"
     gene            5315..8796
                     /locus_tag="PLD82_00500"
                     /pseudo
     CDS             5315..8796
                     /locus_tag="PLD82_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
     gene            complement(8799..9713)
                     /gene="bioB"
                     /locus_tag="PLD82_00505"
     CDS             complement(8799..9713)
                     /gene="bioB"
                     /locus_tag="PLD82_00505"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOG63899.1"
                     /translation="MNEQVRHELGRIARMSANEFVTMVKDLPPRDGPFERCAIINARC
                     GRCSEDCRFCGQSVFATSTIAGWDLIGEEEICSAAREAGEWGVARFGIVVSGRGPDDM
                     DTRRLARLVQAVARDASLPKICLSPGICDKEALLEFKTAGLVRFHHNLETSESFFPNV
                     CTTHTWKERMDTLVTARELGLELCCGGLFGLGESFLDRVDLAMQLAELAPESVPVNFY
                     VPVPGNQMDGLKPLSRDEIIRTLAMLRLALPQSSIRLCGGRTLLGQGLEDAIRHLGLG
                     ALMTGNYLLTKGSSYESDRQMLDRLGVC"
     gene            9760..10260
                     /locus_tag="PLD82_00510"
     CDS             9760..10260
                     /locus_tag="PLD82_00510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG63900.1"
                     /translation="MTSKQPLVSTCLDESGQLLMLSVSPVFSGEISTRLLASLPDFVA
                     DGVADFFAGQDPVLLRIRCSLKSLTDWQQAILRAMTMIPRGRVISYAGLAALAGSSGA
                     ARAAGSACAANPLPLIFPCHRIVHSDGTLGSYMRQAAHPLKRRLLEMEGIELTAEGRI
                     PKEYFL"
     gene            10257..11153
                     /locus_tag="PLD82_00515"
     CDS             10257..11153
                     /locus_tag="PLD82_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOG63901.1"
                     /translation="MNRICYHSLASVLRAWYGRPIPKIGIDAGFGCPNRGLDGRSGPG
                     CVYCNNASFSVSATTGSVQAAITDGLSRLQGSSRWAPQPGIPLMAIAYFQSYSNTWAR
                     PEILRERYTEAISNPGIAGLAISTRPDCIDEQRADILAEFGQKTRLWIELGLQTTSTE
                     ALTWIRRGHSVEDFIKAVRLLQQRDLRTVVHLIPGLPGESSADRDSCARLLADLGIWG
                     IKFHHLQIVRDTPLEELARTDKIPQPRAEEYAGMVAEILARSGDPVVHRLHASCRPEL
                     LSPEDRSDPGIPSLMRKRFIPW"
     gene            complement(11163..12185)
                     /locus_tag="PLD82_00520"
     CDS             complement(11163..12185)
                     /locus_tag="PLD82_00520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG63902.1"
                     /translation="MKRLLFGAGIALPLVFAVFMFVVTRNARPEPEKTPTATNDHSAP
                     VLAGDAEEPVSFELAWKTLQHEEAGHRAAPAPGQENAVRVPVLCYHRIDNVRGDFYHT
                     PREMFEWQMQWLSQNADVIPSSQLVAWVRYRQGLSTTKVTLPPRPVVIHFDDNYRSVY
                     EIAWPILKRYKLPWSFFVYKHHHQPIAEKHLREMAASGVDIQAHSMTHPWFHKPARGQ
                     SMHDYVREMHWQVGGSKKYLEGISERKVHQFAWPFGSYSDLSILLAFQYGFDGMFVAD
                     GGYVTATSSLTSLERVLVTKGWSKEYFKTIVTGKAKLAKVFKPAWLVRNKEEARRLTA
                     PKKEYL"
     gene            complement(12182..12970)
                     /locus_tag="PLD82_00525"
     CDS             complement(12182..12970)
                     /locus_tag="PLD82_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63903.1"
                     /translation="MLQSLSGCCPSFLNGQAGFFLVKRAKGARLHTLDGAVLHDFWLD
                     DGRLPFGHAPRFTGQEQKNHVSTGILSGYAQPVHVRLLARLSRILPGYSLAFIAPSSI
                     HRLEGVPHIKNCSRTAFVFSPSLLADDERPALVLVNGTLGVDILAWLPGQLEELPDPY
                     WPDSVSTVRALSVLARLSGPHAPWPVCAALSERFVKIAGRFVEDRKGSSLRIALDEKF
                     FQPLLNEGLYLARTGWIHFCTEHDPATVDRLARVIVHLKESPCV"
     gene            complement(13055..13429)
                     /locus_tag="PLD82_00530"
     CDS             complement(13055..13429)
                     /locus_tag="PLD82_00530"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG63904.1"
                     /translation="MDGEFSPAKNKHQFIDHLSGGSTTMKSEYDIDNLASLYKALGNP
                     NRLKIYTYLLRATKPENVTNLERALDIKQSHLSQSLNILNSIGLVNRERDGSRVNYSI
                     NKEFLQDFNTFNKNFFKKQGIA"
     gene            complement(13579..>14465)
                     /locus_tag="PLD82_00535"
     CDS             complement(13579..>14465)
                     /locus_tag="PLD82_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOG63905.1"
                     /translation="EIEHSLISRAIANAQKRVETRNFEIRKHLLKYDEVMNEQRTYIY
                     NLRNKVLDSEDSSSIVQEMIIRTVDARIAEYSDDGRKFDSETVESIKRWLEGEMRIPV
                     DIGPVEDIKAINQEEFANSLKKILETLYKAREQSMGSANMRTLEKLVLLNTLDNRWKD
                     HLYEMDALKEGINWMSYAEKDPLVEYKLRGMGIFNEMLTSLDVEAVTLLYHAELTAPQ
                     MPEPEFSQYAQGQASHDEFGQFGAVGGGQSHGGQQSHQAFPGQRQQAVRNANKVGRND
                     PCPCGSGKKYKHCHGTQG"
BASE COUNT         3196 a         3850 c         4210 g         3209 t
ORIGIN      
        1 aagcggagac aatgcccgcc atcggaccga cggcatcatg ctcgtgacag ggagcatatt
       61 cgatttttcc ggatgcagcc agggcttcgg cctcttccgg ggtttttgcc attccctcgt
      121 agatcagggc tccgatgacg gcaccgcgcg tcgggccgga catacgctcc caagtgatgg
      181 gaggaccgga gtggagaatg agattttttt tcatgcccgg gatggtatcg agagccactc
      241 ccattccggt caaaacgggt ttgcccttga gaatgatctc gagggctttg gcattcgctt
      301 cgtcaatctt cgcgagattg tcgtcgatga tgtcgagcga ctccgggctg acaccgaccg
      361 ggggtttcca gtcaacctgg atgacggaaa caccgcagtg tgccaggtca tccctgaacg
      421 cggtcattcc tgtattgact accttgactt cctggctaaa aagatcgttg atggccatgg
      481 atcacttgcc ctccagtatg cggatgatat tgccggccag ttcgctggcg gcggcgtttg
      541 acggcatcat gatggcaccg gctctttcaa gtcctgcctt gatgcgggat ctgttttgcg
      601 ggtccccgtc cgtcccggta atggaacaga tgatcaggag ttcgggggcg atttttttgg
      661 cttcggtaaa cgcggggacg aactcggaga gcgggtccat gtgggaaccg tacccgagaa
      721 caagatccag gaggaggatg gcgacatcct tgtcggcggc ttcatcggtg atcttcttgt
      781 tgcgcagcga aaaatcgatc atcgggtggg gccggccgac ggtaaactcg tcctcgccca
      841 ggtcgatgat ggtgtgcccg acgcttttcc atgagtcctt gaggcggtat tcggggttaa
      901 ggggagcatt gctgttgatg tatccgatca tgtctttgca gatcaactgt gtttcgtcac
      961 agagcgttcc gcccgaaaag agaccgcgca agtacttccc gcggcatttg ggcgcgaggg
     1021 ctttggcctg ctcgatgatg ccagccttgc ggtccgcgat cttctttttg caatcatcga
     1081 cgctggcccc cttggacagg gcggctgcga tgacggcggt ttcctcaaga gtagaacctg
     1141 cgatggcacc agccgcttcg aggatcttgg gatctcctcc gataaagatg ccgacaacgg
     1201 gttttttgat ggtcttgatt tcatcggcga tcttccgcag gacggattca tgcggaggtt
     1261 tggaaacgag aacaatgacc ttggttgccg gatcggcagc cagggcacgc atggcatcaa
     1321 tgaacatgat tccgccgatg gcttccttga catcacgacc gccggttccg atggcttgcg
     1381 agatcccggc gccttcgttg gaaatgatcg tcatcacttc ctggagtccg gttccggaag
     1441 ccgctacgat tccgcagtcg ccctttgaga ggacgttggc aaaggccagg gggacaccgt
     1501 tgatcacggc cgtaccgcaa tccggaccca tcacaaggag tcccttgtcg tgggcaaacc
     1561 gcttgagctt gatctcgtcc tcgatggaga cattgtctga aaacatcatg acatgcagac
     1621 cctgtttgag cgcccgcatg gcctcgcgac cggcatagcg gccggccacc gagataaggg
     1681 aaatattcgc accgggcagg gcctcgactg cgccctcgag acttttggga gaaaaattgc
     1741 ttccaccctc atcctttttg ttgcggagtg ccttgaaaag atcatcgatc ttgaccaggg
     1801 tctcctcgac aaccttgtca ctgtcggctt tgagggccag gaggaggtcg gtatcgtccg
     1861 cctggtcaaa cagctcgggg atgtagagat tcgcgccgcg gacgatctct ttgttctgcg
     1921 ctgttcccat cacaatcgca gaatcggcga tacccggcag cgcattgacc tctttggaaa
     1981 cgatcatcag gctgacggag tcgaaatact cgcctttttt gacaaaacct ttaagcacca
     2041 ttgtttctct ccttgtgcca agatacaaaa gaaggtgaaa atcccgaata ccggccggcg
     2101 gtcaggataa aaccgctggc cagatcggag ccggacgtgg agccgtgagc cagaacacgg
     2161 cgaaaaagtg tttcgagtct ggtttgtcca tcgggagtgg tcgtatcgac ggaaaggatg
     2221 gcttcggctg catcccgcag ggcaagaggc cagcgaccag cgatcgcatg atcgagaaat
     2281 gtccgtgaaa aaacggaaca tgtcggcaga gcagcccgaa gatgtgtcga gagttgatgg
     2341 atggggcaga ccttccggtt ggtggcattg gcgaagtgca tgccaaagag aacaccacag
     2401 agaaaatcat caccgctcgg agtcaatcca tagccgcttc cgagcagcga ggcaacagcc
     2461 tgtccatact cctcacgcca acaggcttcg gctccatcaa gaaaatgttg gagcatgatc
     2521 cggtcaaagt tgccggaaaa ggcagttgta cgcgaatgat cgaggacaac cgccatactt
     2581 ttttctgggg cttcgctgag gagaatcttt tgaaagagca tgagggccgg ttggagatcg
     2641 attggcatgg aaagttccgg aagctggagt gtctcccgat agcgttgggc cccttcgatc
     2701 gggaggacga gactgtcgag tgagagggcg gtttcggtga tgctgactga ttggacggta
     2761 gaaacccaat ccgatgggag atcgggcgaa agaatgatgg tggacggtcc ggcttgatcg
     2821 gcctggtcgg taatcccgac aatcccggcc tccgtcagga tgtcgatgaa ggcatggaac
     2881 ctggcataga cccggtattc cccgggaaga acggatgcac cgataccgag aaccctcggg
     2941 ctcgaaatac ggcaacgcct cggacagggg tatggggaaa gacccttcat tggccatcct
     3001 tgttcttgtg aggaagaaat tagcacaaaa agcatagtac gggcttgatg gtgatgtcaa
     3061 ggatgagggc gggggcgcca agtctctgac ggatatctgt gatccagtct ggcgcaaaaa
     3121 agcggtctgc ggaaagagga aggagaaacc gggcgctgat gcggctgctc gaagatcaag
     3181 tttgagtata ttgatgtatt gaagcagagt agaaaataca ggagcaggcc atgtcacctt
     3241 tgcacggatc ctttgattcc atgttcagcc cggcacggtt gggagaactg agcgaatcct
     3301 cgcggcagat cggaaacgat cttgacagcc tcaattcccg acttgatttc gccgggtcca
     3361 ataccaaaca attggccatc atgttccgga cactctttca ggctgccgcc agtcgaggcc
     3421 tgatttccga cactgaattc cgcgatcgtc tcgattcagt tgatctgggg gaatggacgc
     3481 agtccggaga gaatagcggg caatgatgct tgcggtttcc gcatgtttcg tgtacagtat
     3541 tccaatagag gagggaagta tgccattttc cgaccaatta ttcagcaatt tgtttcctgc
     3601 agccaagatg cgcgagcttg aagatctcaa tcgcaaggaa tatgaagatg taaaaaacga
     3661 catagacagc atcaagcagc ggcttgaaga tcttgaggtg tgcaacaagc agcttgggat
     3721 catgttcagg accttgtacg atctggccat tgaaaagaat atgatcacac gtgaagaatt
     3781 cagggatctt ctggataaag tcgatctctc cgagtgggtt tgaagtcccg tatcgtcggg
     3841 gtcagaatcc gttcttccgt tctgtcgagg gcgccacttc cgtggtcgcc ctcttgtttt
     3901 tttccggtac ttgaataccc aaaaatgacg acaattccag ggggtgagac taccggtttc
     3961 ttcctcgcag gcggccaaag aatcttctga tcggcgcgaa aatggcgttg aggattccct
     4021 ttcgtccatg tttgtcacag gccatcatca gggcatgtat tgcggcatct tcgaggacac
     4081 tccagagtct cgtccggtcg gtggcatagc gcaagaactc gaggggaatc tggaacgccc
     4141 ctttatgtct tctggccccc ccatgatgcg tgatgctctt gatgatccgt tcaagatcaa
     4201 gatctccgtc ccggctgcgg agagaggcat ccaggacaag cgtcccacgg gccggatcct
     4261 cgatgcctgc aaacacgaca acggcttcgg cctcccggtg tgcctccagg agccggtcag
     4321 cgacaatggc aatcgtatcg cgggatgtgg cgtcaatata gcccaggccc gttattaacc
     4381 agcctttgta ggataccgcg tgtttttcgg cttcctcctg ttcatgcagg atctcggacg
     4441 agagcgttgg gagactgatt ctccggacca gtcgatcgtc agcatggtgc gacaggagac
     4501 caagggctgc acgatccata tccccggcaa gacgcatctg gtcggtgtct gtcaggatcc
     4561 cggccaggag ggcactcgcg agaaggcgtt ttttgtcatg ctggaatcgg gcattctcgt
     4621 caaagacggc aacagcgatg atggttgagg ctgcgccaca ggctgtattg atggaacgga
     4681 ccttggcgac cggttggttc gggaacggtg tgtggtgatc gatatgcaga gagcagggga
     4741 cccactccgg cagagtctca agtcgggcat cgggataatc gacgatacag tacccatccc
     4801 ccttccggac agcgcgtttc aaggtctcca gcatgtgcaa ccgcaggccc agcacgcgga
     4861 ccagggcccg gttttgcgag agggatggtg gctgttcgat atatacgagg gatggcaccc
     4921 cggctgtctc tccgagaagc gccagggccc aggctgaggc cagagcatcc ggatcgggag
     4981 agccacgaat gatgatgtgc tgtcttttgc aggaggacaa ggcgtccaac agggtctgca
     5041 gtgtaaggga ggattccata cagacaattt tctacccggt tggcggttcc gtcaaggcaa
     5101 acattgtgcc aattggagga agaaacctgt attttgtagc acaaaatcta aataggcgca
     5161 agatgttgtg cccgcttgcc ttttgctcga tcgatagcta gacttgtgca ggccgacggc
     5221 ccattcactc gagaatttct ttgcacaaca tctccgcatc cgcgggcgta tacatacgtg
     5281 ggttgaatcg tattggagtc ttccgggagg aatcatgcag catacacgac gttttacttt
     5341 aagcggcgaa ggtccttggt caaagctcga gtttgaaaag cgggagtctt cgattcgaaa
     5401 tccggacggc aaaatcattt tttccatgga tcctgtgatt gtcccggcga gctggtccca
     5461 ggtggccacg gacattattg cccaaaaata ctttcgcaag gccggtatcc cggccatcac
     5521 gcgtcgtgtc tccgagcagg gtgtccctga atggcttcag cgttccgaag ccgatgagga
     5581 tgccctggcc gcctggcctg ccagtgagcg gtatatccac gagaacgatg cccgtcaggt
     5641 gttccaccgg atggcaggaa cctggacctg gtggggctgg aagtacagat atttttcctc
     5701 cgaggaagat gcccgtattt tctatgatga aatctgctac atgctggcaa accagatgtg
     5761 tgcgcccaac tcgccacagt ggttcaatac cgggttgaac tgggcgtacg gaatcaacgg
     5821 cccggcacag gggcattata cggttgaacc ggatactggc gaggttcgtc tttcccagga
     5881 tgcgtacacc catccccagc cccacgcctg cttcatccag tccgtcgagg acgatcttgt
     5941 gggtccggac gggatcatgg acctgtggac ccgggaggcc cgactcttca aatacggttc
     6001 ggggacggga acgaattttt cggcgattcg cggtgaggga gaacctcttt cgggaggcgg
     6061 gcactcttcg gggttgatga gctttctcca gattggagac cgtgccgcgg gagccatcaa
     6121 aagcgggggg acgacccgac gagcggccaa aatggcgatt ctcgatcttg accaccccga
     6181 gattgaccgg ttcattgact ggaaagtgat cgaagagcaa aaagtcgccg cacttgttgc
     6241 cggatcacgc atcatgcgtg acacgatgca acggattgtg gcggcctgcg gcttggtctt
     6301 ggatgccgac gcgatcgatc ccaaaaaaaa tctccagctc gctgaagcgc tttcacaggc
     6361 cagaaagagc catatccctg atgcctggtt gatgcgcatt ctgcaactca ttcaagaagg
     6421 aaaaagcgaa atcgatgtcc cggtgtacga tacggactgg aatgccgaag cctatcacac
     6481 ggtatcgggg cagaattcaa acaactcggt ccgcattccc aatgaattca tgcaggccct
     6541 tgaacaggat ggtcattgga acctcgtcgg acggatggaa aaagcacagg cccgggctga
     6601 aaaccggact cccaaggcga tgcggaccat agcggcccgg gagctctggg acaagatcgc
     6661 ccacgctgcc tggtcatgtg cggatcccgg tgtacaatat catacgacta tcaatgaatg
     6721 gcacacctgt cccgaagacg gtgagatcag ggcatccaat ccctgttccg aatacatgtt
     6781 cctcgacaat acggcctgca atctggcgtc actcaatctt gtgaaatttt acgatcagga
     6841 aaccggtggt tttgatgtgg ccggtttcag gcatgccgta cggctttgga ccattgtgct
     6901 cgatctcagc gttctgatgg cccagtttcc cggcaagagc atggcggaac tctcctggaa
     6961 attccggacg accggtctcg ggtatgccaa tatcggcagc ctggtgatga tcatgggcta
     7021 cccctatgat tccggtgaag cgagggctgt ggcagcatcc attactgcga tcatgcattt
     7081 ttcggctttg tccgcttcct ccgagatggc ggcagaactc gggacgttcc ccggattcca
     7141 ggccaacagg cgccacatgc tgcgggtcat cagaaaccac cgggctgcag catatgacag
     7201 cgagagttcc gagtatcagg ggctgaccat caaacccctc gggattgatg aaaaacgggt
     7261 tccggcctat cttgttgcgg cagcacgcca agaggctgat gcggcgcttt cctccggtga
     7321 agcacatggc tatcggaacg cgcaagtcac cgttttggca cccaccggga cgattggcct
     7381 tgtgatggat tgtgatacga ccggtgtcga acccgatttt gcgctgatta aattcaagaa
     7441 actggcaggg ggcgggtatt tcaggatcat caaccaatcc attccgccgg cactcaaaca
     7501 tcttggatat agcgaagagg agatcgcctc gatcatcgac tatgtcaagg ggcacgggac
     7561 cttgatcggt tgtcaatcca tctcccacga ccggcttgtc gcaaaaggct tcgacaagca
     7621 gtccttgaag aagatggaga gcctgttgcc gacagcattc gatatctcct ttgtcatcaa
     7681 ccccggcatt ctcggtgaag agttttgccg cacgacgctc ggtgtggaca gagcaatgct
     7741 ggaagcggct ggcggaagtc tcctgaccgc gcttggcttc tcgagagagg agattgatgc
     7801 cgccaatgac ccggtgctcc ttcgatcatc atcgttccac aaacccagtc attggcggca
     7861 tcacgctgcg ggaaacgcgg aacccgcatg atcgctccga ccgggcacat gatgatgatg
     7921 gcagccgtcc agccattcat ctccggcgcg atctcgaaga caatcaatct tcccttctcc
     7981 gcgtccatgg aagatgtcaa gcggatctat gccgaatcct ggaagctttc tctcaaggcg
     8041 gttgcgctct atcgggatgg ttcgaaactc agtcagccgc tcaatgccat cgcggaatct
     8101 gtccttcttg agggtcttga ggagattgtc cgttccgagg ctccgcgtca gcagcaggta
     8161 gtccagattg tcgaaaaagt cacccaatac atcgccaagc gccggaagct ccccagcaga
     8221 cggaacgggt atacgcaaaa agcacggatc gggggacact ccatctatat ccggaccggg
     8281 gaatacgaga acgggcaact gggtgaagtc tttatcgaca tgcatcggga gggggccgca
     8341 ttccggagtt tgatgaactg ctttgccatc gctgtctccc tcggactcca gcacggggtt
     8401 ccgctcgaag aatttgtcga ggcctttgtc tttacccgat ttgagccgaa cgggatggtc
     8461 atgggtcatc aacacgtcaa gatgaccaca tctgtcgtgg actatatctt cagggatctg
     8521 gccatttcct atcttggccg gacggatctg gcgcacatga ccccgggtga cacgattcgc
     8581 gaaacggata ttgcgactcc tgaaacacgg gcaaaaatcg aggggccggc gacgccaggt
     8641 ccggctgccc aggaagccaa tgccgacaag gtccgtcagg cggttctcca gggctatacc
     8701 ggagacattt gcagctcatg cggatcgatg accatggtgc ggaacggaac ctgcctcaag
     8761 tgtatgacct gtggtacgac cagcgggtgc agctgagttc aacagacacc gagccggtcc
     8821 agcatctggc ggtccgactc gtagctgctt ccctttgtca gaagatagtt gcccgtcatc
     8881 aacgcaccga gtccgagatg acgtatggcg tcttccagac cttgaccaag gagggttctt
     8941 cctccacaga gccggatgga tgactgcggg agggccagcc gcaacatggc cagtgtccgg
     9001 atgatctcgt cacgcgacag cggcttgagg ccatccatct ggtttcccgg gaccgggaca
     9061 taaaaattga ccgggacgct ttcgggagca agttcggcga gctgcatggc cagatcgaca
     9121 cggtccaaaa aactctcacc caaaccgaag agaccaccgc aacagagttc gaggccgagt
     9181 tcgcgggccg tgacaagggt gtccatgcgc tctttccagg tatgggttgt gcatacattc
     9241 ggaaaaaaag attcggacgt ctcgaggttg tgatgaaaac ggaccagacc ggccgtcttg
     9301 aattcgagca aggcctcttt gtcacagatt cctggcgaga ggcagatttt ggggaggctg
     9361 gcatcacggg caacagcctg cacgagccgg gcgagccggc gggtatccat gtcatccggg
     9421 ccacggccgc tgacgacgat accaaaacgc gcaactcccc actcgccggc ttcacgggcg
     9481 gccgagcaga tctcttcttc tccgatcaaa tcccagccgg caatggttga cgtggcaaac
     9541 acggactgcc cgcagaatct gcagtcctca ctgcatcggc cacagcgggc attgatgatc
     9601 gcacagcgtt caaacggacc gtcacggggt ggcagatctt tgaccatggt cacgaattca
     9661 ttggcactca tcctggcaat tcgtccaagt tcatgcctga cttgctcgtt catgtgctac
     9721 cttttcacgg ggatgaatgt aaaggtacgg gatgatgtga tgaccagcaa gcaaccactt
     9781 gtctcgacat gtctggatga atccgggcaa ttgctcatgt tgagtgtctc acctgtgttt
     9841 tcgggagaga tcagcacacg tctgttggca agcctgcccg attttgtggc cgatggagtg
     9901 gcagattttt ttgccggtca ggacccggtg cttcttcgaa tccggtgttc tctgaaatcc
     9961 ctgacagact ggcagcaggc tattctccgc gcgatgacga tgataccacg ggggcgggtc
    10021 atcagttatg ccggtcttgc agcattggcc ggatcatccg gggcagccag ggctgcaggg
    10081 tccgcctgtg ccgccaatcc gctccccctg atttttccct gtcaccggat cgttcactcg
    10141 gatgggacac ttggatcgta catgcggcag gcagcacatc cgttgaaaag gcgtctcctc
    10201 gaaatggagg ggatcgagtt gaccgctgaa ggtcgcattc cgaaggaata ttttttgtga
    10261 acaggatttg ctaccattcc ctggccagtg tccttcgtgc gtggtacgga aggccgattc
    10321 cgaaaatcgg gatcgatgcc ggattcggct gtcccaaccg ggggctcgac gggcgttcag
    10381 gtcccgggtg cgtgtattgc aacaacgcct ctttttcggt ttcggcaaca acaggcagcg
    10441 tgcaggccgc aattaccgac ggtctctccc gactgcaagg ttcaagccgc tgggcaccac
    10501 aacccggcat ccctcttatg gccatcgcct acttccagag ttacagcaat acctgggcac
    10561 gtccggagat tctgagggag cgctatacgg aagccatcag caatcccggc atcgcaggtc
    10621 tggccatttc gacacgtccg gattgcatcg acgaacagcg agcggacatc ctggccgaat
    10681 tcggacaaaa aacacgactc tggatcgaac tgggtttgca gacgaccagt acagaagcgc
    10741 tgacctggat ccggcggggg cacagtgttg aagactttat caaggcagtc aggcttctgc
    10801 agcaacggga tctgcggaca gtcgtgcacc tgattcccgg tcttcccgga gaatcatcgg
    10861 ctgaccggga ctcgtgcgca cgcttgctgg cggatcttgg tatttggggc atcaagttcc
    10921 accatctcca gatcgtacgg gacacgccgc ttgaagaact cgcccggacc gacaagattc
    10981 cgcaacccag ggcagaggaa tatgctggga tggtggccga aatcctggca cggtccggcg
    11041 atccggttgt ccacagactt catgcgtcct gccggccgga actcctgtca ccggaggaca
    11101 ggagtgatcc aggaatacca tccctgatgc ggaaacgatt catcccctgg taacagccgg
    11161 gatcagagat attctttttt gggggccgtc aaacgcctgg cttcttcttt gttccggaca
    11221 agccaggcag gcttgaacac cttggccagt ttggccttcc ccgtcacgat cgtcttgaaa
    11281 tattcttttg accacccctt ggtgacaagg acgcgttcca gcgaggtgag actcgaggtg
    11341 gctgtcacat acccgccgtc agcaacgaac ataccgtcaa agccgtattg gaaggcaagc
    11401 aggatggaga gatcgctgta ggacccgaag ggccaggcaa actggtgcac cttcctctcc
    11461 gagatgccct cgagatattt tttagagcca ccgacctgcc agtgcatctc gcggacatag
    11521 tcatgcatgg attggcctct ggccggttta tgaaaccagg gatgcgtcat gctgtgtgcc
    11581 tggatatcca ctccggatgc ggccatctcg cgaagatgtt tttcggcgat cggctgatgg
    11641 tggtgtttgt acacgaaaaa actccatgga agcttgtagc gcttcagaat cggccaggcg
    11701 atttcgtaca cacttctgta attgtcatca aagtggatca ccaccggcct cggcgggagt
    11761 gtgaccttgg ttgtcgagag tccctgccgg tagcgtaccc aggcaacaag ttgcgatgac
    11821 ggaatcacat cagcattttg ggagagccac tgcatctgcc attcgaacat ctcgcgcggg
    11881 gtatgataga aatcgccgcg gacattgtcg atcctgtgat agcagagaac cggcaccctg
    11941 acggcgttct cctgtccggg tgccggggcc gcacgatgac ccgcttcctc gtgctggagg
    12001 gtcttccagg cgagctcgaa cgaaactggc tcttcagcat cacccgcgag aaccggagcg
    12061 gagtgatcgt tggtggccgt cggagttttc tccggctcgg gacgggcgtt ccgggtgaca
    12121 acgaacatga agaccgcaaa cacaaggggc agggcgatgc ctgctccgaa caagagacgt
    12181 ttcatacgca tggactttcc ttgagatgta caatcactcg tgcaagacga tccacagtgg
    12241 ccggatcgtg ctctgtgcaa aaatgtatcc accctgtccg ggcaaggtac aatccttcat
    12301 tcaggagtgg ttggaaaaat ttttcgtcga gagcgatgcg cagggaagac cccttgcggt
    12361 cctcgacaaa ccgaccggca atcttgacaa accgctcgga caaggcagca cagacaggcc
    12421 aaggggcatg gggaccggag agtcgtgcca gaaccgacag ggcccggact gtcgagacag
    12481 aatccggcca gtatgggtcg gggagttctt cgagctggcc gggaagccat gccaggatat
    12541 cgacaccgag cgtgccgttg acaagaacga gcgccggcct ctcgtcatct gccaggaggg
    12601 agggcgaaaa gacaaatgct gttcgcgagc agttttttat gtgaggaaca ccctcgagcc
    12661 tgtggatgga tgatggcgct ataaacgcca gggagtatcc gggcaggatg cgggaaagac
    12721 gtgccaggag ccggacatgg accggttgcg cgtatcccga gagaataccg gttgaaacat
    12781 ggtttttctg ctcttgcccg gtaaagcggg gagcgtggcc gaaggggagc cgtccgtcat
    12841 cgagccaaaa atcgtggagg accgctccat ccagagtgtg gagtctggct cccttggctc
    12901 tcttgacgag aaaaaatccg gcctgtccgt tcaagaatga cggacagcaa ccggagagtg
    12961 attgaagcat cccattgctt tgcgaaaaag tgaccggagt acgatgtgcc attaatacag
    13021 taaggtactg cttccgatct taaaaaggaa caattcaggc aatgccctgc ttcttgaaaa
    13081 agtttttgtt gaaggtattg aaatcctgca ggaattcctt gttgatgctg tagttgacac
    13141 ggctcccatc gcgttcgcga ttgacaaggc cgatagagtt cagaatattc agactctgtg
    13201 aaagatgtga ctgtttgata tcgagtgctc tctcgagatt cgtaacattc tcgggttttg
    13261 ttgcgcggag aagataggta tagatcttca gacggttggg gttcccgagg gccttgtaga
    13321 ggctggcgag gttgtcgata tcgtattcgg acttcatggt tgtacttcct cctgataaat
    13381 ggtcgatgaa ctggtgcttg ttttttgcgg gcgagaattc tccatccata cggaccacaa
    13441 tacatgacta gcataatcag tacttcgcaa ttattcaagc aaaaaacaca cagctatttc
    13501 tgaattcgtg atgaagaaaa accccccgtg gaagaccgga gggctatctt gaaaacattg
    13561 tacggattct gaaagtcgtt atccctgggt tccgtggcag tgcttgtact tttttccgct
    13621 tccgcagggg caggggtcat tccgcccgac cttgttggca ttcctgacgg cctgttgccg
    13681 ctgtcccgga aaggcctgat gggactgttg tcccccgtgc gactgcccgc caccgacggc
    13741 cccgaactgg ccaaactcgt cgtgactggc ctggccctgg gcatactggg agaactcggg
    13801 ttccggcatc tggggtgctg tcagttccgc gtggtagagc agggtgacgg cctcgacgtc
    13861 caggctggtc aacatctcgt tgaagatccc catcccgcgc agcttgtact cgacaagagg
    13921 atctttttcc gcgtaggaca tccagttgat cccttccttg agcgcatcca tctcatagag
    13981 gtggtccttc cagcggttgt caagagtgtt caacagaacg agcttttcga gcgtccgcat
    14041 attggcgctg cccatcgact gttcgcgggc cttgtacaga gtctcaagga tctttttgag
    14101 agagttggca aactcctcct ggttgatggc cttgatatcc tcgacggggc cgatatcaac
    14161 gggaatccgc atttcgcctt cgagccagcg cttgatggat tcgaccgtct cgctgtcaaa
    14221 tttgcgtccg tcatccgaat actccgcaat ccgggcgtca acggtccgga tgatcatctc
    14281 ctggacgatg ctggaagaat cctcgctgtc gaggaccttg tttcgcaggt tgtagatata
    14341 ggtccgctgc tcgttcatca cctcgtcgta cttcaagagg tgcttgcgga tttcaaagtt
    14401 tctggtctcg acacgttttt gggcgttggc tatggcgcgg ctgatcaggg agtgctcgat
    14461 ctcct
//
LOCUS       DAOSHE010000007        12922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4050_length_12922_cov_3.815186, whole genome shotgun sequence.
ACCESSION   DAOSHE010000007 DAOSHE010000000
VERSION     DAOSHE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_12922_cov_3.815186"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PLD82_00540"
     CDS             complement(<1..1498)
                     /locus_tag="PLD82_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG63906.1"
                     /translation="MGIETGKTGSITVSDGEVIISQGQTVKSLYILTSGKIEIVKTAS
                     GLDISTDDEVLLTEGYRLGQLGQNTFPGIPNLFDGKPASCSYRALGPCSLYIAPIQNE
                     QQLQSIMASKPDHAMAMAMTMMLGIDALSQFWTRLQRGHDTLSIMVENAGVYYFLLKE
                     IIGSKSTVHSSYFRQAEQNLAALRLRDIPVPEEFSQPFMEKDFSAIYGEERLSPPQNN
                     KKQVEYYRRFARLAPNLRKAFFTADPAMTLYICHDLSRCFGQLMDDVGSLVDRYDELF
                     DTIYNLHKESLFTEFARAAIDAIRTNSDSVLPSQIIDYLSIRTKELLSGFASEFGKDP
                     GIDFGYFERALQQDDINAVAEGVETSGLSPDIAALLDDDAGVDDSADVAIIPDETPTV
                     EEIVDVSSLPDELKGSLKKILDYSAISKEKADQFKAAMDSFRKLKDKFSSEDPVRKLR
                     RAVTTIFYDVYEAVFKRMHKERNTSRLYTMFINYGYMDERLLNPATTMR"
     gene            complement(1663..2337)
                     /locus_tag="PLD82_00545"
     CDS             complement(1663..2337)
                     /locus_tag="PLD82_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG63907.1"
                     /translation="MIPTALITGASGKLGNRIAAHLSARGFHLVLHGFGHTPCMHAAD
                     DEVLRADLSGWEGVKILERRLGEGDIGLFVNCASLFMRGSYSELDFQQLSRIFAINVH
                     APTVLTRALALGRMGAGNPVPGHVIHLLDSKILLRNSRYAAYTLSRKTLVQAAEMAAL
                     EYPGILRVNAICPKEIGLPETGETIENLLRIMDGLLDDQSSNSRCLFLDGSERALASF
                     FPEEGF"
     gene            complement(2334..3401)
                     /gene="hisC"
                     /locus_tag="PLD82_00550"
     CDS             complement(2334..3401)
                     /gene="hisC"
                     /locus_tag="PLD82_00550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG63908.1"
                     /translation="MKTRYWNERLRGMAAYTPGFQPPIKAETIKLNTNENPWPPSPLA
                     IQAMVESIGSELRLYPPSAWVELRAAISETYGIGSDRIFCGNGSDEILSLVFRCFTDP
                     GDSLLLPWPTYSLYPVLAAAAGCTVEYVETKDDFTIDLDTMLGRKARIAILTNPNAPT
                     GILAPPEGLLDFADRFEGLVVIDEAYIDFAPEGSSTLAGLGTRENVIILRTFSKSFSL
                     CGIRCGYAFAAPGLVEGLVAMKDSYNLDLVAQRGATAAIRDIAWMKENAARIVATRRD
                     FVIALESMGCRVLPSDANFIFMQHGSVPAGLIQQMLAEQDIHVRHFSGKRVADWVRIS
                     IGTDDEMQRVRTVLARVLAGA"
     gene            complement(3404..4450)
                     /locus_tag="PLD82_00555"
     CDS             complement(3404..4450)
                     /locus_tag="PLD82_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63909.1"
                     /translation="MQGSNDIAMRFFTGRSPAWLVLAAVSVVFSSSMFAAEPVNHWRR
                     DVRVALSNPVQSLQELILVNDLDGNGTVEAVVRHVDLHRGQDAEFRIYEVTVRNGRGE
                     RGREIARVKTSDDYCQGIEFRDCDGDGKNELICMTIGRDGLRRGLHIVRIDRETQSFT
                     ILQPDQPLGQIEAVHSFQPKTASGPAELTVISVSSSDETVFSPVAASAGQMPKLWMRQ
                     VYQIEPNALRLTESVMHETPYYVLTKMIQALQRKDMFAAYKYMFADAAYHDFRKQTIQ
                     NFPYICAKQPASQFILAEWGLEIFRDSRTQGWLTFTHVFEENGRARRMMYQAFMRKVY
                     DEWKITLLRKIQDL"
     gene            4593..5969
                     /locus_tag="PLD82_00560"
     CDS             4593..5969
                     /locus_tag="PLD82_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG63910.1"
                     /translation="MKYSRVLALVVLLPCLAFLDCGSDDAESPEIPLLRLDPPGIHAS
                     GYPDEVPHDLPYLACDFSVPCATNSGYCVLLGPTNIPCTRTWSVSGERLYLTVGLLPP
                     GRYTLSLGGFLSTHAQEFKEYTGSFSVRIRQPGNDTTPPVLVSQTPADQDFSVQPSLA
                     RIVLRFDEPVDTTALEYSLSDGVKNLRCTIDTSMRMTDLVICTLDEPLTPETCYVFSL
                     YSIADPAGNARPVHVLLFATASSGGTQGHLAEGLVEHPVLSEVCVAGYSAGGASDEFI
                     ELYNPGMTPLDLAAGGYRVYKASATGSPELICDFANANHFSRLASPTRIPAQGFFLIA
                     NQASGTALAEIADGLVLKSRMSLAANNTVWLTRGGVPSESTSIDCIGMGQATRYEGPL
                     PAAQPASGASLERKARQDSDPASMGSGGRDEMRGHALDRNRNSDDWVLRSLPQPQGTD
                     SGYEDWTR"
     gene            complement(5977..6120)
                     /locus_tag="PLD82_00565"
     CDS             complement(5977..6120)
                     /locus_tag="PLD82_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63911.1"
                     /translation="MVFRVIDRNGNCLIHTPTEKIARRLISDNLEREYRLVRTAGDTS
                     IGR"
     gene            complement(6163..9060)
                     /locus_tag="PLD82_00570"
     CDS             complement(6163..9060)
                     /locus_tag="PLD82_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG63912.1"
                     /translation="MDGRSKRIFIRGVIVLSILPVIFLLVHTLAMHETGYRLQERQLK
                     NLVHQISSEVEQRGMTVVSIAGMASRFLPGQEALFEKMIQALPGGQWYGGLALREADG
                     TLLSSRTGTSQVQIRIQADRGRPEFENETNGYLVFSSAACSTPCLVARAMIGQGRWLS
                     LYFSSEVLGLEYSIRRLRSLGIRAVLTLENRQIFASSFQQRLEGLPLSVLLPIREGVD
                     GTDKQDFLPERGRKQLLIVTNGAKQEGINMETVWETIRFPGERLRLYLLTPEERALID
                     ARPSFVYTIFLHAFVVVISLVAIGFFIFLYRRLGRIREDAAQIPELRQNVTRLKETSL
                     RYSTLFNSMIDGILLLDMHGRILDCNRTFAEMLGYETGELIGTNQRLLTPPRFWQTEN
                     ESVFAQVSSWGFTKDYEKEFIRKDGSTVPVMINSCLIRESDSKNGSILSTLRDISEQK
                     RALDEINRLNLLLEDIIESSTLCILTLDERLRIRMVNSSAERFFRIERAMVMGSPLFE
                     SIPFFERLRSLFERTISENKPMFQGNETVVFGENDEKHVNITIYPLHSRESGSVAIHL
                     EDITDHVHLERRLFQSQKMEALGTLASGFAHDFNNLLSGIFSYLTVIKMKTKDEEVLE
                     PLDLVYSIAKRASTLVKHIVTFSRSSQIRAVPVNIGSVIDEVVAMIRESFPKNIDLQT
                     VACDDDCWILGDQSQIAQVLLNLCINARDAMPSGGVLAISLERLRISEEDARRFRLLK
                     PGNTVRVTVSDTGMGIDPAIADKIFDPFFTTKSDRNGTGMGLFIVYGILRSHRGDIEL
                     SSTPGKGTSISIFIPLSEPPENGETLPENEACSQKTGNTELRGRLVVIDDEEIIGGAL
                     REGFSRMGFEVRTALNGAEGLRLCRESRPDMVILDMIMPGLGGEETFDLLKAEYPDLK
                     IVIHTGFTQGDVEQRMLEKGALDVLHKPYEMTEVAQRLAAWIRQGQAQGNP"
     gene            9191..9748
                     /gene="efp"
                     /locus_tag="PLD82_00575"
     CDS             9191..9748
                     /gene="efp"
                     /locus_tag="PLD82_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG63913.1"
                     /translation="MYDTTDITKGLMVEIDGNPYTVVDFQFVNPGKGSAFTRCRFKNL
                     LTGAVLDRTLKSGEKINEANVEERMMEYLYFNGESYTFMDNATYDQIEISSSKVGDNA
                     GFMKENTVVKVLFYNDEPVTVTLPTFVELEVTETIVADKGNTVTTSYKPATLSTGGTV
                     QVPMFVKNGDILKIDTRDKSYVERL"
     gene            9732..10970
                     /locus_tag="PLD82_00580"
     CDS             9732..10970
                     /locus_tag="PLD82_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HOG63914.1"
                     /translation="MSSVSEQPDRITGRVTAVDQQCIRLWREDQILTFFLGNNLQPIC
                     APGDLVEVSLDKSGTCTGLSVLAKPIAGHDDPLRWRKPDRRGRTRMEHLRFRHALIRR
                     VEAWFDKAGFLQVQMPALLPSVNPEAHFQFIRGEDHILSTSPELQMKRMLVGGFEKIV
                     SLGACYRGHERSARHNPEFWMLEWYRVGASLEMVMEDTEEIVRVTAHLSPWVDSDGNL
                     HLEDGTHVALSGPRWPRVPVSQHILQETGVDIDGCLDVSSLGDRMRSRGMDVPDDEEF
                     EQLFSRLWAASEPGLPRETPCFVTDWPAPVASLARLSPGRPWVAERAELVIAGLELSN
                     GFCELTDSREQAARFAAETVTRSHRGLPPVPSDGTFIASLNAGMPPSAGMALGFDRLV
                     MLLSGAASIRDVLTFSSDEL"
     gene            complement(11005..11766)
                     /locus_tag="PLD82_00585"
     CDS             complement(11005..11766)
                     /locus_tag="PLD82_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG63915.1"
                     /translation="MENKRMYNPLLLLLCLVRLVLVVVLTAIILPVVILARFAGHTYP
                     HACARIWARLVLLVLGVRIHKTGTDRDWSPRSVIISNHLGYLDIPVLLVSLRIPFRFI
                     ADSDVFRVPLLGLAMRFCGYLPIWRSGHNRINKTMQAAMTFVRNGIPVVIFPEGGINR
                     TADGEGYCRVLSGFASIARETGSRVHQVTLCGTGDAQRNLCRFTKKTASMLLESHIEI
                     PPEDKKMPREAQSVLAKAIDARSREAFGRIRGFCD"
     gene            11827..>12922
                     /locus_tag="PLD82_00590"
     CDS             11827..>12922
                     /locus_tag="PLD82_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOG63916.1"
                     /translation="MDTQRNWRIALLGTPNSGKTTLFNALTRSREHVGNYPGVTVDRA
                     SGLFSHHGQNFIIEDLPGLYSIHAPSAEERVSVTALFDSIPDLTLVACDATNLERGLH
                     LVLQIIELGIPLCVVITKTDVLTQIGSSLDVGLLSKLLKCPVFTTETPESEAGIERFF
                     DVLLGAVLNPGIPPAFRYPNEIENEIIRVQDALINPDRQIPFVRAVATALLSSLDSPV
                     IDASGFVPGNEIHELVDQSHHHVRTVFDESPAMTITKARYGIVRGMLRESLSPDTEAD
                     YSLSDFLDRLFLWRLTGIPMLLLTLFVIFFIPFVAGAPLSSLVDSLFTDLAGLVRSGF
                     EPGAMRDLIADGVIGGVGGVLVFVPQLFLLY"
BASE COUNT         2795 a         3651 c         3607 g         2869 t
ORIGIN      
        1 gacgcatggt agttgcggga ttgaggaggc gctcgtccat atacccgtaa ttgatgaaca
       61 tcgtgtagag tctggaggta ttccgttcct tgtgcatcct cttgaagacg gcctcgtaga
      121 catcgtagaa aatcgtggtg acggcgcgac ggagtttgcg gacaggatct tcggaactga
      181 atttgtcctt gagcttgcgg aaagaatcca tggctgcttt gaactggtcc gctttttcct
      241 tggagatcgc ggagtagtcg agaatctttt tcaatgaacc cttcagctcg tccggaagac
      301 tggagacatc cacgatctcc tcgacagtcg gagtttcatc cgggatgatg gccacatcgg
      361 cactgtcatc gacaccggca tcatcgtcga ggagtgcagc gatgtccggg gagagtcccg
      421 atgtttccac gccctcggcc acggcattga tgtcatcctg ctggagtgcc cgctcaaaat
      481 acccgaagtc gattccggga tcttttccga actcgctggc aaaaccggac aggagttcct
      541 ttgtcctgat ggagaggtaa tcgatgatct gggaaggaag aacgctgtcg ctgttggtcc
      601 ggatggcatc gatcgccgcc cgggcgaatt cggtaaacag gctctccttg tggagattgt
      661 agatggtgtc aaagagttca tcatagcgat cgaccaggga gccgacatcg tccatcaact
      721 ggccgaaaca ccgggagaga tcatgacaga tatagagggt catggccggg tcggccgtaa
      781 aaaaggcctt cctcagattg ggggccagcc ttgcaaatct ccggtaatac tcgacttgct
      841 ttttgttgtt ttgtggcggc gagagccgtt cttctccgta aatggcagaa aaatcttttt
      901 ccatgaaggg ctggctgaat tcctcgggga cagggatgtc ccgcaaccgg agtgccgcca
      961 gattctgttc ggcctgtctg aaataggaac tgtggacggt actcttggac ccgattattt
     1021 ccttgagaag gaaataatag actccggcgt tttcgaccat gatcgagagg gtatcgtgtc
     1081 cacgttgcag tctggtccag aattgggaga gtgcgtcgat cccgagcatc atggtcatgg
     1141 ccatggccat ggcatgatcc ggtttgctgg ccatgatcga ctgtaattgt tgttcgtttt
     1201 ggatgggtgc gatatagagg gagcacggac ccaaagcccg ataactgcag gaagccggtt
     1261 tgccgtcaaa gagattgggg attccgggaa aggtgttttg cccgagttgt cccaggcgat
     1321 agccctcggt caggaggact tcgtcgtccg tcgagatatc caggccggat gctgtcttga
     1381 cgatctcgat cttgcctgag gtcaggatgt acagagactt gaccgtctgt ccctgactga
     1441 tgatgacttc accgtcactg accgtgatac tcccggtctt gcccgtctcg attcccatgg
     1501 atcctgactc ctcgataaaa agctgcccgg tccctgtatg acacaacagc acgtttgtcc
     1561 accgcgtgcc agatgtttcc cggatagata aggaagaacg ctcttccagc caccccggaa
     1621 caggcctcta ccattgtagc aagaagacat gcttctccgc aatcaaaatc cttcttccgg
     1681 aaaaaaagaa gccaaggcgc gttccgatcc atccagaaaa aggcaacggc tgttgctgga
     1741 ctgatcgtcc agaagtccat ccatgatcct gagcaggttc tcgatggtct cccctgtctc
     1801 aggtaagccg atctccttcg gacagatggc attgaccctg aggatgccag gatattcgag
     1861 agcggccatc tcggcagcct ggactagggt cttccgcgag agcgtgtagg cagcatatct
     1921 tgaattccgg aggagaattt tggaatcgag aagatggatc acatgccccg ggacgggatt
     1981 cccggcaccc atcctgccga gggccaaagc ccgggtcaga actgtcgggg catggacatt
     2041 gatcgcaaaa atccgcgaga gttgttgaaa gtccaattcg gaataggacc cgcgcatgaa
     2101 gagcgaggca cagttcacaa aaagaccgat atcgccctcg cccagtcttc gttcgaggat
     2161 cttcacgccc tcccagccgg agagatccgc ccgcaagacc tcgtcatcgg ctgcatgcat
     2221 gcagggtgtg tgtccgaaac cgtgaagaac aagatggaac ccgcgggcgg aaagatgggc
     2281 ggcaatccgg ttaccgagtt tgccactcgc tccggtgatg agcgccgtcg ggatcatgct
     2341 cctgccagaa cccgtgccag gacggtccgg acccgctgca tctcgtcatc ggttccgatc
     2401 gaaatccgca cccagtcggc tacccgtttt ccggaaaaat ggcgcacgtg gatgtcctgc
     2461 tcggcaagca tctgctggat gaggccggcc ggaaccgaac cgtgctgcat gaagatgaaa
     2521 ttggcatcgc tgggaagcac gcggcatccc atggactcga gagcgatcac gaaatctcgt
     2581 cgggtcgcaa cgatccgcgc cgcgttttcc ttcatccatg cgatatcccg gatggcggct
     2641 gtggcaccgc gctgggcgac aaggtccagg ttgtacgagt ccttcatggc aaccaatccc
     2701 tcgacaagac cgggtgcggc aaaggcatag ccgcagcgga taccgcaaag ggaaaaagat
     2761 ttcgaaaacg tccggagaat gatgacattc tcccgtgtgc ccaatccggc aagggtggaa
     2821 ctcccctcgg gggcaaagtc gatgtaggct tcatcgatga ccacgagccc ttcaaacctg
     2881 tcggcaaaat ccaggagtcc ttcgggcggg gcaagaatcc ctgttggtgc attcggattc
     2941 gtcagaatgg cgattcttgc tttccgcccg agcatggtat ccaggtcgat ggtaaaatcg
     3001 tcttttgtct cgacatactc caccgtgcac ccggccgctg ccgccagaac cggatacagg
     3061 gaataggttg gccagggcag gaggagcgaa tcgcccggat cggtgaagca gcggaaaacc
     3121 agcgagagga tttcgtcact gccgttcccg caaaatatcc ggtcacttcc gattccgtag
     3181 gtttccgaga tggctgcccg caattccacc caagcggagg gtggatacag tctcagctcg
     3241 ctcccgatcg actcgaccat cgcctggatg gccagaggtg atggcggcca tggattttcg
     3301 ttggtattga gtttgatggt ctcagccttg atcggaggct gaaaacccgg ggtataggcc
     3361 gccatgccgc gcagccgctc gttccagtac cgtgtcttca ttgtcagaga tcctgtatct
     3421 tgcgaagcaa ggtgattttc cattcgtcat agaccttgcg catgaaggcc tggtacatca
     3481 tccgcctggc ccgtccattc tcctcgaata cgtgcgtgaa ggtcagccag ccctgggtcc
     3541 gggagtcccg gaaaatctcg agaccccatt cggccaggat gaactggctg gccggttgtt
     3601 tcgcgcaaat gtagggaaaa ttttggatgg tctgtttccg gaaatcgtga taagcggcat
     3661 cggcgaacat gtatttgtac gcggcgaaca tgtccttgcg ctgaagggcc tgtatcattt
     3721 ttgtcaggac gtaatagggc gtctcgtgca tgacactctc ggtgagcctg agtgcattgg
     3781 gttcgatctg atagacctga cgcatccaga gttttggcat ctggccggcg cttgccgcaa
     3841 ctggagaaaa gaccgtctcg tctgaggaag aaactgaaat cacggtcagt tcagccggtc
     3901 cggatgcggt ctttggctga aatgaatgaa ccgcctcaat ctgccccagg ggctggtcgg
     3961 gttgaaggat cgtgaaggat tgtgtttccc ggtcaatccg gacgatgtgg agtccgcgcc
     4021 ggaggccgtc tcggccgatg gtcatgcaga tcagttcatt ttttccgtca ccgtcacaat
     4081 cgcggaactc gattccctga cagtagtcat cagacgtctt tactcgggcg atttcccggc
     4141 ctctctcgcc tctcccgttg cgtactgtga cctcatagat ccggaactcc gcgtcctgcc
     4201 cgcgatggag atccacatga cgcacgacgg cttcgacagt cccgtttccg tcaagatcgt
     4261 tgacgaggat cagttcctga agggattgta cggggttgga gagggcaacc ctgacatccc
     4321 ggcgccagtg attgacgggt tcggcggcga acatcgaact gctgaaaacg acggaaacgg
     4381 ctgccagaac cagccaggca gggcttcgtc ctgtaaagaa tcgcatggcg atgtcattgc
     4441 tcccttgcac gtggatattt cgagactcca tatccgataa tcggcatatt atttcaacaa
     4501 tacaaaaaaa acatggaacc gtggcgatcg cccggacctc tccttcttga acggcttcgg
     4561 gttccaaccc gccccgtcag aaaggagatg tcatgaaata ctctcgtgtt ttggcattgg
     4621 tggtcctgct tccatgtctc gcattcctcg actgtggcag tgatgatgcg gagagcccgg
     4681 agataccgct cctccgcctg gatccacccg gcatacatgc ctcaggctat ccggacgagg
     4741 tcccgcacga tctcccctac cttgcctgcg atttttcggt cccatgtgcc acaaactccg
     4801 ggtattgcgt cctgctcggt ccgacaaaca ttccttgcac acggacctgg tcggtctcgg
     4861 gagaacgcct gtatctcaca gtcggcctgc tcccgccggg aaggtatacg ctctcgttgg
     4921 gcggttttct gtcgacacat gcacaggaat tcaaggagta caccggaagt ttttccgtcc
     4981 ggatccggca acccgggaac gatacaactc ctccggtcct cgtcagccag actcccgccg
     5041 atcaggattt ttcggtccag ccgtctcttg ccagaattgt cctgcgcttt gacgaacctg
     5101 ttgatacgac tgcgctggaa tactccctgt cagacggggt aaaaaacctg cgctgcacca
     5161 tcgacacaag catgcgcatg accgatctgg tgatctgcac gcttgatgag ccacttactc
     5221 ccgaaacctg ttatgtgttt tcactctact ccatcgcgga cccggcaggg aatgcacggc
     5281 ctgtccatgt cctgctcttc gcaacggctt cttcaggagg aacacagggg caccttgcgg
     5341 agggattggt ggaacacccc gttctgtcgg aagtctgtgt cgccgggtat tccgcagggg
     5401 gagcctcgga cgagttcata gaactctaca atcccggaat gacgccactc gacctcgcgg
     5461 cgggaggata tcgagtctat aaagcgagtg ccacaggctc acccgaactg atctgtgatt
     5521 ttgcgaatgc caaccatttt tcacgcttgg ccagtccgac caggattccg gcccaggggt
     5581 ttttcctgat tgcaaatcag gcgagcggaa cggccctcgc ggagatcgct gacggtcttg
     5641 tcctcaaatc gaggatgtcc ctcgccgcca acaatacggt ctggctgacc cggggtgggg
     5701 tgccttcgga atcgacaagc atcgactgca tcgggatggg acaggccacg agatacgaag
     5761 gcccccttcc ggctgcccaa cccgcctccg gtgccagtct ggagcgcaag gccaggcagg
     5821 attcggatcc tgcctccatg ggaagcggcg gccgtgacga gatgcgtggt cacgccctgg
     5881 acaggaacag gaattccgat gattgggtgc tccggagtct tccccagccc caaggtactg
     5941 acagcgggta tgaagactgg acccgttgac cgggcattat cggccgatgg atgtatcccc
     6001 ggccgtccgg acgagccggt attcccgctc tagattgtcg gaaatcaggc gccgggcgat
     6061 tttttctgtc ggagtatgga tgagacaatt gccgttgcga tcaatgacgc ggaaaaccat
     6121 gggaacctcc aggatgacgg gctaggctat ccgtcggcag gatcaggggt tcccttgagc
     6181 ctgtccctgg cggatccagg ccgccagcct ctgggcgact tcagtcattt cgtagggttt
     6241 gtgcagaaca tccagtgcgc ccttctcgag catccgctgt tcgacatccc cttgggtaaa
     6301 cccggtgtgg atcacgatct tgaggtcggg atactccgcc ttgaggagat cgaatgtctc
     6361 ttccccccca aggcccggca tgatcatgtc caaaatgacc atgtccggcc ggctctcccg
     6421 gcagagacgc agcccctctg ctccgttcaa cgctgtccgg acctcgaatc ccatccggct
     6481 gaatccttcc cgcagggctc ctccgatgat ctcttcgtca tcgatgacaa cgagtcgccc
     6541 ccgcaactcg gtatttccgg ttttttgtga acaggcctca ttttccggga gggtttctcc
     6601 gttttcgggt ggttcggaaa gcgggatgaa gatgctgatg gatgtccctt tgcccggtgt
     6661 gcttgacaac tcgatatccc cgcgatggct gcggagaatc ccgtacacga tgaagagtcc
     6721 catccctgtc ccgttccggt cactcttggt ggtaaaaaat gggtcgaaaa tcttgtccgc
     6781 gatggccgga tcgatcccca tccctgtatc gctgacggtc acacggactg tgtttccggg
     6841 cttgagcagt ctgaaccggc gggcatcctc ctcactgatg cggagtcttt caagggagat
     6901 ggcgagaaca ccgcccgagg gcatggcatc ccgggcgttg atgcagagat tgaggaggac
     6961 ctgggcgatc tgtgactggt ctccgagtat ccagcaatca tcatcacagg caacagtctg
     7021 caagtcgata tttttcggaa aggactcccg gatcatggcg acgacttcat caatcacgga
     7081 gccgatattc acgggcacag ccctgatctg gctgctccgt gaaaaggtca caatgtgttt
     7141 gacaagcgtt gaagcccgct tggcaatact gtatacaaga tcgagcggtt cgagaacttc
     7201 ctcatcctta gtcttcatct tgatgactgt caggtaactg aagatccctg aaagaaggtt
     7261 gttgaagtca tgggcaaatc cgctggccaa tgtccccaag gcctccatct tctgggactg
     7321 gaagagccga cgttcgaggt ggacatgatc cgtgatatcc tccagatgga tggccacact
     7381 cccggattcg cgtgaatgga gcggatagat ggtgatattg acgtgcttct cgtcattttc
     7441 accgaataca acggtctcat tgccctggaa catcggcttg ttttcactga tcgtccgttc
     7501 gaacagactg cgcaaacgtt cgaaaaaggg tattgattca aagagcggag aacccatcac
     7561 catcgcccgc tcaatccgga aaaaccgttc ggcagaactg ttgaccatcc tgatgcgcag
     7621 ccgctcgtcc agggtcagga tacagagggt actcgactcg atgatgtcct cgaggagaag
     7681 attcagccgg ttgatctcgt ccaaggcacg cttctgttcg gagatatcgc gcagggtcga
     7741 gaggatcgat ccgttttttg aatcggattc gcggatcagg caggaattga tcatcaccgg
     7801 aaccgtactc ccgtctttcc tgataaattc tttttcgtaa tccttggtaa agccccagct
     7861 cgagacctgt gcaaagacac tctcgttctc ggtctgccaa aaccggggtg gtgtcaaaag
     7921 tcgctggttt gtaccgatca gttcgcccgt ttcgtatccc agcatttcgg caaaggtccg
     7981 gttgcagtcc aggattctgc cgtgcatgtc cagaagcagt atcccgtcaa tcatcgagtt
     8041 gaagagggtc gaatagcgca ggctggtttc tttgagtcgc gtgacattct gccgcaattc
     8101 cggaatctgg gcggcatcct cgcggatccg tcccagtctt ctgtaaagga aaatgaagaa
     8161 accgatggcg acaagactga tgacgacaac gaaggcgtga aggaagattg tgtatacaaa
     8221 ggaaggtctg gcatcgatca gggcccgctc ttcgggagtc agaagataga gccggagccg
     8281 ctcaccggga aagcggatgg tctcccaaac ggtctccatg ttgatgcctt cctgtttggc
     8341 tccattggtc acaatcagga gctgtttccg tcccctttcg gggagaaaat cctgcttgtc
     8401 cgtaccatcg acaccctccc ggatcgggag cagcacggag agaggaagac cttcgagacg
     8461 ctgctggaag gaactcgcaa agatctggcg gttctccagg gtcaaaacgg cacggatccc
     8521 gagagatcgc agtcgccgga tggaatattc cagtcccagg acctccgatg aaaaatagag
     8581 actgagccat cgtccctggc caatcatggc cctggcaacc aggcaggggg tgctgcaggc
     8641 agcactggag aaaaccaggt atccgttggt ttcgttttcg aattcaggcc gtccccggtc
     8701 agcctggatc ctgatctgca cctggctggt tccggtgcga ctggacaaga gggttccatc
     8761 ggcctcacgg agtgccaagc caccgtacca ctgccccccc ggcagtgcct ggatcatttt
     8821 ttcaaaaagc gcttcctgtc cgggaagaaa gcgggaagcc atccctgcaa tcgaaacgac
     8881 tgtcatgccg cgctgctcga cttcggaaga gatctggtgc accagattct tgagctgacg
     8941 ttcctggagt cgatatcctg tctcatgcat ggccaaggta tggacgagca ggaagatgac
     9001 tgggaggatc gagaggacaa tgacacccct gatgaaaatg cgttttgacc ttccatccat
     9061 gcacctggcc tcccgggaac atctcgcagc gcttccccaa attgtacgcc caaaaaccgg
     9121 aaaatatgcg tactttttac tggagcatac acatgccaac caacccagat tttgtcagga
     9181 gaaccgaaca gtgtacgata cgaccgatat taccaagggg ctgatggtcg agatagacgg
     9241 aaacccttac accgtcgttg atttccagtt tgtcaacccc ggcaagggaa gtgccttcac
     9301 ccgctgccgt ttcaaaaacc tcctcaccgg agctgtgctt gaccggaccc tcaagtcagg
     9361 ggaaaagatc aacgaagcca acgtcgaaga acgcatgatg gagtacctct attttaacgg
     9421 cgagagctat accttcatgg acaacgcgac gtatgaccag atcgagattt cttcgtcgaa
     9481 ggttggcgac aatgccggct tcatgaagga aaacacggtt gtcaaagtcc tcttttacaa
     9541 tgacgagccg gtgacagtca cccttccgac ctttgtcgaa ctggaagtca ccgagacgat
     9601 tgtcgccgac aagggcaata cggtgacaac ctcctacaag ccggccactc tctcgaccgg
     9661 cgggactgtc caggttccca tgttcgtcaa aaacggagac atcctcaaaa tcgatacccg
     9721 tgacaagagt tatgtcgagc gtctctgagc aaccggaccg catcacgggt cgtgttacgg
     9781 ccgtcgatca acaatgcatt cgtctctggc gtgaggatca gatcctcacg ttttttttgg
     9841 ggaacaatct ccagccgatc tgtgcgccag gagatcttgt cgaggtttcg ctggacaagt
     9901 cgggaacgtg caccggtctg agtgttctgg caaagccgat cgcaggccac gatgatccct
     9961 tgcgctggcg taaaccggac agacgcggtc gaaccagaat ggaacacctc cgtttcaggc
    10021 acgcccttat ccggagggtc gaagcctggt tcgacaaggc aggctttctc caggttcaaa
    10081 tgccggcact cctgccttcc gtcaatcccg aagcccactt ccagtttatc cggggtgagg
    10141 accacatcct ctccacctct cccgagctgc agatgaaacg gatgcttgtc ggcgggttcg
    10201 aaaaaattgt ttcgcttggc gcctgttacc gagggcacga acgttccgcc aggcataacc
    10261 cggaattctg gatgctggag tggtaccggg tcggggccag cctcgagatg gtcatggagg
    10321 ataccgagga gattgtccgg gtaacggcac atctctcgcc ctgggtcgat tccgacggga
    10381 atctccacct ggaagatgga acccatgttg cactttccgg tccccgctgg cctcgggtac
    10441 cggtcagcca gcacatactg caagagaccg gcgtcgacat cgatggctgc ctggatgtgt
    10501 catcgcttgg agacaggatg cggagccggg gcatggatgt gccggatgac gaggaatttg
    10561 aacagctttt cagcagactc tgggcggcca gtgaacctgg cctgccccgg gagactccct
    10621 gcttcgtcac ggactggccg gctcccgtcg ccagcctggc cagactctct cccgggcggc
    10681 cttgggtggc cgaacgtgcc gaactggtca ttgcaggact cgaactctca aacggatttt
    10741 gcgagctgac cgattcccgg gaacaagctg cccgctttgc tgccgaaacg gtcactcgat
    10801 cgcaccgtgg acttccccca gtcccgtctg acgggacctt catcgcatcc ctgaatgccg
    10861 ggatgcctcc ctccgccggc atggcgctcg ggtttgacag gctggtcatg cttctctcgg
    10921 gtgccgcgag catccgggat gttctcacct tcagcagtga cgaactctga ccggaagcgg
    10981 gatcattcgt caacacagga ggattcagtc gcagaaaccc cggattcgcc cgaaggcttc
    11041 acgggatcgg gcatcaatcg ctttggccag aacggactga gcctctctcg gcattttttt
    11101 gtcttccggc ggaatctcga tgtgactctc caacagcatg ctcgcggttt ttttggtgaa
    11161 ccgacagaga ttccgttgcg catcacccgt gccgcagagc gtgacctggt gaacacgcga
    11221 accggtctcg cgggcaatcg atgcaaaacc ggagaggaca cggcaatatc cctccccgtc
    11281 tgcggtccgg ttgatcccgc cctcgggaaa aatgacaacc ggaatcccgt ttcggacaaa
    11341 ggtcattgct gcttgcatcg ttttgttgat gcggttgtgc ccgctccgcc agatcgggag
    11401 atagccacag aagcgcatgg ccaatcctaa aaggggcaca cggaagacat cactatcggc
    11461 tatgaaccgg aacggaatcc tgagcgagac aaggaggacg gggatatcaa ggtacccaag
    11521 atggttcgag atgatcactg agcggggcga ccagtcccgg tcggttccgg tcttgtgaat
    11581 cctgactccg agcacaagga ggaccagtcg agcccagatc ctggcacagg catgagggta
    11641 cgtgtgacca gcaaaccggg ccaggatgac cacgggtaga atgatggcag tcaggaccac
    11701 gaccagcaca agccggacca gacagaggag caacagcaag ggattgtaca tgcgcttgtt
    11761 ttccatattg ttcaccttaa cacgaagagt gtcattttgt ccaaaattgt cgaaattgta
    11821 gctataatgg atacgcaacg aaactggcgg atagccctgc tcggcactcc aaattccggc
    11881 aagacaaccc tcttcaatgc tctgacccgt tcccgcgaac atgtcggcaa ctatccgggg
    11941 gtcacggttg acagggccag cggcctcttc tcccaccatg gccagaattt catcatcgaa
    12001 gacctgcccg ggctctacag tatccacgca ccttccgccg aggaacgggt cagtgtcacg
    12061 gccctcttcg attcgatacc cgatctgacc ctggttgcct gtgatgccac aaacctcgaa
    12121 cggggcctgc accttgtcct gcagatcatc gaactgggca tcccgctctg tgttgtgatc
    12181 accaaaaccg atgtgctcac ccaaatcggg tccagccttg acgtcggact tttgtcaaaa
    12241 ctcctcaagt gtcctgtgtt cacaacagaa acacctgaaa gtgaagccgg catcgaacgt
    12301 ttttttgatg ttcttctcgg tgctgttctc aatccgggaa tcccgccggc tttccgctat
    12361 cccaacgaaa tagaaaacga aatcatccgc gtccaagatg ccctgatcaa cccggaccgg
    12421 caaatcccgt ttgtccgtgc agtcgccacc gcgctgctct cctccctgga ctcacctgtc
    12481 atcgatgcca gcggatttgt tcccgggaac gagatccatg aacttgtcga ccagagtcac
    12541 catcacgtgc ggacggtgtt cgatgaatct cccgccatga cgattaccaa ggcgcgctat
    12601 ggaatcgtcc gtgggatgct tcgcgagagt ctcagtccgg ataccgaggc cgattacagt
    12661 ctttcggact ttctcgacag actttttctc tggcggttga ccgggatccc catgcttctc
    12721 ctgaccctgt tcgtgatttt cttcattccc tttgtcgccg gagcccccct ttcgtccctt
    12781 gttgacagtc tcttcacgga tctggcgggc ttggtccgtt ctggtttcga acctggcgca
    12841 atgcgagacc tgatcgcgga cggtgtcatc gggggagttg gcggggtact tgtctttgtt
    12901 ccacagctct tccttctcta cg
//
LOCUS       DAOSHE010000008        12872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4070_length_12872_cov_4.117110, whole genome shotgun sequence.
ACCESSION   DAOSHE010000008 DAOSHE010000000
VERSION     DAOSHE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_12872_cov_4.117110"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1182
                     /gene="ribD"
                     /locus_tag="PLD82_00595"
     CDS             88..1182
                     /gene="ribD"
                     /locus_tag="PLD82_00595"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOG63917.1"
                     /translation="MDISITPMLDCYWMEQALREARGTLGRTWPNPPVGAVLVKDGLC
                     IARGATQIPGHNHAERECLARAGDSALGATLYVTLEPCSHHGRTPPCTEAVIQSRVAR
                     VVVATLDPNPLVSGQGIRSLRDAGIAVETGCLEEEARNLMQPFFKYITTGRPWIVAKY
                     AMTLDGRMALDSGDSFWISGEKARAEVQVLRKDAQAILVGGNTVRQDNPRLDCRIASG
                     EWQPARIILTAQGIPPDARLFSGGGPVHILAPEDKVSGILAGLGTRASVHGLSSLDPG
                     PVIAWLGEHGFASVLVEAGPGILTGLVREGYVDRVRVYIAPVMAGGTGMQPIRDIGTS
                     LMQMAPRLRGTWRNVGSDICFEGDFVPEEE"
     gene            1333..2526
                     /locus_tag="PLD82_00600"
     CDS             1333..2526
                     /locus_tag="PLD82_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG63918.1"
                     /translation="MAWRTAQDAFEAGNIREARRQAFIAADKGMPIARMKLLLAATAW
                     YQGRRRDAQEILDNALACGQVGPEAYYYIANNYRLQGNLKAALENYRLVLKGSQDILY
                     RALTLREIEQMHMLENLQFSRLVALCSREIDSEDSYATLSERRGYAILAMLRGDTHGA
                     FIRLRNLQRDWPDTAEIYRDFAMLHIRDGEWLRAIIDLKKGCRLYPGDYEMHVELARC
                     LHRVGRLEEALAVLRSMKGDALLNPAIFVNMGNIHIRQGRSVGAIRAFRKALELRDDY
                     IPALFNLGTVYHQAGSLDLAEQYYRRILTIESHHAWAMYNLALLYYESGDYATAVRML
                     KRCHDDNPGFEPAMRNLRFINLARVCFPNEGPEELKTRTEHAWWIGAASLGIVVIFSI
                     LKGWV"
     gene            2529..3062
                     /locus_tag="PLD82_00605"
     CDS             2529..3062
                     /locus_tag="PLD82_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63919.1"
                     /translation="MGNEEAVMHLAEVNRIFGDVMKDCTPTMLPDERFRAALSALIVM
                     DDEDAADAFASVLGAINQKRNIHFLNYLLQRAEPFVKRWLFFLLDELNTTLEIYFSGN
                     NLKRAFRILEIISGYSDARTLAILQSYIEGCRDSRIVRMLEEYRKKAEERISMCMKRI
                     EYLDVDPIITHYEEWQS"
     gene            complement(3208..4236)
                     /locus_tag="PLD82_00610"
     CDS             complement(3208..4236)
                     /locus_tag="PLD82_00610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOG63920.1"
                     /translation="MQPNEFFYQLTPDAMLTALEQAGYHPEGRFLMRNSLENRVISVA
                     LSDSGSCVAKFYRPGRWSRETILEEHAFLFELAAHEIPVCAPLLSHGTSLHEYQGIFY
                     AVWPLTGGRGLEDPDEDTMRQIGRLLGRIHAAGSRQGFASRVEFSALSYVERPQAFLE
                     PLMPVHCRPRYRNAARRIAELYTTESQGVPVQRIHGDCHPGNLLIDGSNIFFLDFDDC
                     LTGPVVQDLWMLLPDRGDASSRLKTAFLEGYTLFATFDPAWFRLIEILRGMRFVHYAA
                     WIARRREDPAFMLAFPDFGTDAWWERETRDLEEQVQIIEETLSEKTDQGQEKTYSNFD
                     YFPDLRDP"
     gene            complement(4527..5618)
                     /gene="add"
                     /locus_tag="PLD82_00615"
     CDS             complement(4527..5618)
                     /gene="add"
                     /locus_tag="PLD82_00615"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOG63921.1"
                     /translation="MHKNKPKGPSAEIIRALPKVELHRHLEGAFRLETLLDLARKRNL
                     VDLPLDDVDAFRRVVQMTEHDLPDFLTFLSKFRADWYSTLEDPRRVAYEAVIDAAKEN
                     ILYFEMRFSPEHYARKSGFALEAVIETVIAGAKQAADETGIMIAFLVTLGREKLDADE
                     MKRYIKVCQPYLDHGVVGIDLAGDESHYPAELFSSVFKGLYERTGFKSTIHAGEALGP
                     ESVVTAVRDLDAHRIGHGVRTIEDQAAMDLILEKKIPLEMCLTSNLQTGTVSSLKKHP
                     FPKFFKQGVRVTLNSDDPQIQQSDLNDDYGHAASLYQMTIEDFRTINRYSLEGAFQPD
                     DVRHTLLARYEARFDAALLECGVSRETVP"
     gene            complement(5738..6505)
                     /locus_tag="PLD82_00620"
     CDS             complement(5738..6505)
                     /locus_tag="PLD82_00620"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG63922.1"
                     /translation="MKSRSFSLLTLVVPLMTLILILSPSKAAAFPFSPGEVIRFQIKV
                     FGLHVGYQDMKFTGPVMLNGRRVLHAVADTRSLESIKSAFNYSLHDVIEVWMDPVTLL
                     PIRVYKDIQEGSWTDKVTIDIDQNNRTALYRDKRNPNGVRIPLRRATLDILSLVYYVR
                     AQQAQPGTMLSADYIVDNKQGVKDANIAVRRADPLPVGGRSVPTLLFEQRGGQGVRVR
                     MTDDANRVPVSITVGTFKVYGYDIDIVGSLVKVTRGQ"
     gene            6597..7829
                     /locus_tag="PLD82_00625"
     CDS             6597..7829
                     /locus_tag="PLD82_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG63923.1"
                     /translation="MSCLHPFRSVRPRPDLAREVASPPYDVLSSEEARAMAGSNPHSF
                     LHINKSEIDLPEGTDLYSDAVYARAKENFARMRSEGIFVQDEKPCLYVYTLEEGSHRQ
                     SGIAGCFSAQEYLDNHIRKHEHTRKDKEEDRTRHIDETNAQTGVVLLTFKAHADVKSL
                     VEKVTARKPLYDFTAEDGIRHIVHLIDDAGEISALVKYFEGINPLYIADGHHRSAGAS
                     IVYERRKAANPGHTGNEEYAYFLAVAFPDNEMRILPYNRVLVDLNGMTKEGFFAKLGE
                     KFEIAPTQDKAPKQDKEVSMYIGGEWFGLRYNGSLPADPLARLDVAIMQNEVLGPLFG
                     IEDPKKDKRIDFVGGTRGTAELEKLVDSGKFAVAFSMFPTSIGELMAVAEEKKIMPPK
                     STWFAPKLRDGLLVHVLE"
     gene            7816..8406
                     /locus_tag="PLD82_00630"
     CDS             7816..8406
                     /locus_tag="PLD82_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63924.1"
                     /translation="MSLNKAGLILVVLMACGFSFAAGGLAVWLAGPEHAGPEMYRIAG
                     RSYGRDAALAEFAVTMKMSSQFSPAQRTRFSTNTAFRNLWLRNRYLSDILAAHAGSSG
                     WLDSAECQAWLRLAVREAVRQYVLVRHLDDVTVSSNEVIRWYDAHARSLQQLPLDEAM
                     VYAGERALLEKRQDALMERTLEYAKKLDAIFKTTNL"
     gene            8421..9074
                     /locus_tag="PLD82_00635"
     CDS             8421..9074
                     /locus_tag="PLD82_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63925.1"
                     /translation="MASSETGQIRVIWRPVFLLPREARRILVEAADADSDRRYGIWRP
                     SLLVGGTVALFALLFLGGSGLTASNIFITETVPATPLRVLLLLIAGCIGILFFFFAIR
                     ILAKRLGHSRYYLYLGDEWLVERNLWHVSIIPASDLVYAWGRREFRTRGADLEYESLV
                     WRDGNGSLKVYDLHDHYWHGAGHGRQVASSWNLIRKQYRIGGPPAGFGAGKMHRKET"
     gene            9074..9757
                     /gene="queC"
                     /locus_tag="PLD82_00640"
     CDS             9074..9757
                     /gene="queC"
                     /locus_tag="PLD82_00640"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOG63926.1"
                     /translation="MDNALVLASGGLDSTTVLAQAVADGFAVTTLSFRYGQKHDIELS
                     RAREISSRYGAVRHLELELDPRPFASSALTSGGEIPLDRTHESMGAAIAPTYVPARNM
                     LFLTMALALAEGLPARDIFIGVNHVDYSGYPDCRPAFIEAFEKAANLGTSAADGGRPF
                     RIHAPLMQLSKAGIIHLGISLGVDYSHTVSCYLADDQGRACGHCDACLLRKQGFAEAG
                     VPDPTRYRR"
     gene            10170..11369
                     /locus_tag="PLD82_00645"
     CDS             10170..11369
                     /locus_tag="PLD82_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63927.1"
                     /translation="MDMQIQRLFSDRVAGLCRFFPAIVLTGARQSGKTTLLRQLFPGY
                     AYVSLDLPDAAARAEHSPEEFFARYQAPLIVDEVQYAPGLFRHLKTVIDADRTTPGRY
                     ILTGSQLFPLMQEVADSLAGRCAVLELENLTLSEIRQAEVREYTLAERIVRGQFPELW
                     RQQDLPSNEYYASYLATYLERDVRQIMNVRSLRDFERFLRILASRSGNMLNMSDVARD
                     VGVSVKAIGDWVSILEASGQIALLEPWFTNFGKRLVKTPKLYFRDSGLLCFLLGLNPS
                     GLLESPFLGAVWETFVFAELRKGAGLAQHPGKFWYYRDQRAREVDFLYENNGRLDFME
                     CKWMEHPGKKETAILATVADELDRSSLPWKSAARRILTSGSTSWPLAPGIEAVGYPEI
                     MGLVMER"
     gene            complement(11657..12799)
                     /locus_tag="PLD82_00650"
     CDS             complement(11657..12799)
                     /locus_tag="PLD82_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63928.1"
                     /translation="MERRTAALPERIRHFTRERSALPRALLLVGPRGTGKTTFLLHHA
                     KNSRLLYFSADNPTLAGTALYETVKTIFQAGYKGVIIDEVHFARDWSRHVKALYDEFP
                     RHGIWISDSSALVLRDGVGDLSRRFVRINMPLLSFREFLFLETGSEYPIFDAFSGGKK
                     LPVAPDADILAAFRRYRECGTRPFYAEGDFKDRLLAVLEKSLYFDIPFFLPQITDGNL
                     RLMKAIVGTLAGSAIPRLQVRSLCTDWGIGAEKLYQIVNVMESVGILSVIRAENETKT
                     QSVGAKLFFSDPALYPVLAGNPGTAREALVACLCAASGWTVEASRDERAGDFVLTRAT
                     GGKRKKVTIEIGGTTKSRKEADFVIRDDLDYPAARAIPLWLLGMGY"
BASE COUNT         2720 a         3474 c         3868 g         2810 t
ORIGIN      
        1 cgggtcttgc gcttcctttt ccggactgaa atccggatca gcagagcaag gcttcgactt
       61 tcgttccggt acccgtatac ttgcaggatg gatatctcga taacacccat gctggattgc
      121 tattggatgg agcaggcttt gcgggaagcc agggggactc tgggccggac ctggcccaat
      181 cctccggtcg gtgccgttct cgtcaaggac ggattgtgca ttgccagggg ggcgacacag
      241 attcccggtc acaaccatgc cgaacgtgaa tgcctggccc gggccgggga ctcggcactt
      301 ggcgcaaccc tctatgtgac acttgaaccg tgcagccatc atgggagaac ccctccctgt
      361 acggaagccg tcattcagtc ccgggttgcc cgggttgtcg tcgctacgct ggacccgaat
      421 ccccttgttt ccggtcaggg gatccgttcc cttcgggatg cgggaatagc tgttgaaacc
      481 gggtgccttg aggaggaagc ccggaacctg atgcaaccct ttttcaagta catcacgacc
      541 ggcaggccat ggatagtggc aaagtatgcg atgactctgg acggccggat ggcactcgat
      601 tcgggggatt ccttctggat cagcggagag aaggccaggg ctgaagtcca ggttctccgc
      661 aaggatgccc aggccatact ggttggcgga aacaccgtgc gacaggacaa cccgagactg
      721 gattgccgga ttgcctccgg ggaatggcag ccggcccgga tcatcctcac ggcacaggga
      781 attccgcccg atgcccggct cttttcaggc gggggcccgg tccatattct tgccccggag
      841 gacaaggtca gcgggattct cgcagggctg ggcacacggg ccagtgtgca tggtctttcg
      901 tcccttgatc ccggcccggt cattgcctgg cttggtgaac acggatttgc ctccgtgctt
      961 gttgaagccg gcccgggtat cctgaccggg cttgtcaggg aggggtatgt cgatcgggtc
     1021 agggtctata tcgctccggt gatggccggt gggaccggga tgcagcccat ccgggatatc
     1081 gggacaagcc tgatgcagat ggcaccccgg ctcaggggaa catggcgcaa tgtcggttcg
     1141 gacatctgtt ttgaaggcga ttttgtaccg gaagaggaat gatccgggcc ggagtgaagg
     1201 ctttttcgcg aaagttgcac tccttgactg ccgttaattg aagaagaaac acaatggaac
     1261 gtggttggtg gcggtgtgat ggctggagtc cgtacaacag gacagaatga tgccttgatc
     1321 agcaagtctg agatggcttg gcgtacagcc caggatgcgt ttgaagcggg aaacatccgg
     1381 gaagcccgcc gtcaggcttt cattgctgcc gataaaggga tgccgatcgc caggatgaaa
     1441 ctcctgctgg cggccacggc ttggtatcag ggccgacggc gggatgctca ggaaattctg
     1501 gacaatgccc tggcctgcgg tcaggtcggt cccgaagcct attattacat tgccaataat
     1561 tacagactcc aggggaacct caaggccgcc ctcgaaaact atcgactggt tctcaaaggt
     1621 tcgcaggata tcctctaccg cgcgctgaca ctgcgcgaga ttgaacagat gcacatgctc
     1681 gagaatctgc agttctctcg tctggttgca ctctgcagca gggaaatcga cagcgaggac
     1741 agctatgcga cgctctccga gcggcgaggg tacgccattc tggccatgct ccggggcgat
     1801 acccatggcg ccttcatccg gttgagaaat ctccagcggg attggccgga cacggcggag
     1861 atttaccgcg attttgcgat gctccatatc cgggacgggg agtggctccg ggccatcatt
     1921 gacctcaaaa aagggtgccg gctctatccg ggtgattacg agatgcatgt cgaactcgcc
     1981 cgatgtcttc accgggtggg gagactcgaa gaagccctgg cagtcctgcg ttcgatgaag
     2041 ggtgacgcac tgctcaatcc ggccatcttt gtcaatatgg ggaacatcca tatccggcag
     2101 ggccggagtg tcggtgccat ccgggcattc cgcaaagccc tggagcttcg cgacgactat
     2161 atcccggctc tgtttaatct tggaaccgtg taccatcagg ccgggagtct tgatcttgcc
     2221 gagcagtact atcgcaggat cctgacgatc gagtcccatc atgcctgggc catgtacaac
     2281 ctggcactgc tctattacga gtccggtgac tatgcaacgg ctgtacgcat gctcaagcgg
     2341 tgtcatgacg acaatcccgg gtttgaaccg gccatgcgca atctgcgttt cattaatctg
     2401 gctcgggtct gcttcccgaa tgagggtccg gaggaactga agactcggac cgagcatgcc
     2461 tggtggatcg gggcagcctc gctcggaatc gtcgtcattt tttcaatact caaaggctgg
     2521 gtatgagcat gggaaacgaa gaagctgtca tgcatcttgc cgaggtcaac cggatcttcg
     2581 gtgatgtgat gaaggactgc acaccgacca tgcttcccga tgaacggttc cgggcagcgc
     2641 tctcggcctt gatcgtgatg gacgatgagg atgcggcaga cgctttcgcc agtgttttgg
     2701 gcgcgatcaa ccagaagagg aacatccatt ttttgaacta cctcctccag agagcggaac
     2761 cctttgtcaa gcgctggctc ttcttcctgc tggacgagct caatacgacc ctcgagatct
     2821 atttttcggg gaacaatctc aaacgggcgt tccgcatcct tgagatcatc tccgggtaca
     2881 gcgatgctcg aaccctggcc atcctgcaaa gctatatcga gggctgtcgt gactcccgga
     2941 ttgtcaggat gctcgaggaa taccggaaaa aggccgaaga acggatctcg atgtgcatga
     3001 agaggatcga atacctggat gttgatccga tcatcaccca ctatgaggag tggcagtcct
     3061 gagagcgtgc gcctgctcgt atcctgtcac ccacacagaa acactctgat ccaaaaggat
     3121 ccgtgagacg gcatgacgga ccttggagcg catctgcgtg ttctattgac cggcactgcg
     3181 tgtacagcgg actgctcaag ggagtgatca gggatcgcgc aagtccggga aatagtcgaa
     3241 gttgctgtac gtcttttcct gaccctgatc cgtcttttca gaaagagtct cctcgatgat
     3301 ttggacctgt tcctcaagat cccgggtctc ccgttcccac caggcatccg tgccaaaatc
     3361 gggaaaggcg agcatgaagg cggggtcttc tcttctcctg gcgatccagg cggcgtaatg
     3421 gacaaagcgc atcccgcgca ggatttcgat cagccggaac caggctggat cgaaggtggc
     3481 gaagagggta tagccttcaa ggaaggcggt tttcaagcgg gatgaagcgt caccacggtc
     3541 cgggaggagc atccagagat cctggacaac cggcccggtc aggcagtcat cgaaatcgag
     3601 aaaaaagatg ttcgaaccat cgatcaggag gtttcccgga tgacaatccc catgaatcct
     3661 ctggaccggg acaccctgcg actcggtggt atagagctcg gcaattctcc gggcggcgtt
     3721 ccggtatcgt ggccggcaat gaacgggcat gagcggctcg aggaaggcct gagggcgttc
     3781 aacatagctc agagctgaaa actccacacg ggaagcgaat ccctgcctgg aaccggccgc
     3841 gtggattcga cccaggaggc ggccaatctg gcgcatggta tcctcatcgg gatcttcaag
     3901 tccacgtcct ccggtcagcg gccagacagc gtagaagatg ccctggtatt catgaagact
     3961 ggtgccatgg gagaggagtg gtgcacagac cggtatctcg tgggcagcca gctcaaacag
     4021 gaaggcgtgc tcttcaagga tggtctcccg ggaccaacgc cccggccggt agaatttggc
     4081 aacacaggag ccggaatcgg aaagcgcgac actgatgacc ctgttctcga gactgttgcg
     4141 catgagaaaa cggccttcag gatgatagcc ggcttgctca agagccgtca gcatggcgtc
     4201 aggggtgagc tggtaaaaaa actcattggg ttgcatggct tcctctgggc aaagtatacg
     4261 catggagcac caaagaaaaa acatcccccc gattcaacga accgggggga tgaccacaaa
     4321 aaggaggatt gtgcatgaaa tgcgtactgc cgcttgccac tgaactatat tgcaaggtat
     4381 atgccaatcg caggaaattt gccgaaaaat gataatttgc agaagtataa gcagataaca
     4441 gcgttgtcag gatggaagga ttttttttgg agaacaggag gctgtcaaac tgacacgtcg
     4501 gaagggcgta agacgctctt ttgccattat ggcacagttt cccgggaaac accgcattca
     4561 aggagagcgg catcgaagcg cgcttcgtag cgggccaaca aggtgtgccg gacatcatcc
     4621 ggctgaaacg caccctcaag gctgtagcgg ttgatggtgc ggaaatcctc gatggtcatc
     4681 tggtacagac tggctgcatg gccatagtcg tcattcagat cactctgctg gatctgggga
     4741 tcatcggagt tgagcgtgac acggacgccc tgtttgaaaa atttggggaa ggggtgcttc
     4801 ttgagcgagg aaaccgtccc ggtctggagg ttgctggtca gacacatctc cagggggatc
     4861 ttcttctcca gaatgaggtc catggctgcc tggtcctcga tcgtgcgtac accatgcccg
     4921 atccggtggg catcaagatc acggacggca gtcaccacgc tttcgggacc aagagcttct
     4981 ccggcatgga tcgtggattt gaagcctgtc cgttcataca gccctttgaa gacggaggaa
     5041 aagagttcag ccgggtaatg actctcgtca ccggccagat cgattccgac gacaccgtga
     5101 tcgagatagg gctgacagac cttgatatag cgcttcattt cgtccgcatc cagtttttcc
     5161 cgtcccaggg tcaccagaaa agcgatcatg attccggttt catcggcagc ctgcttggct
     5221 cccgcgatga ccgtttcgat gaccgcctca agggcaaaac cactcttccg ggcatagtgc
     5281 tcgggagaga agcgcatttc gaaatacagg atgttttctt tggccgcgtc gatcacggct
     5341 tcgtaagcga cgcgccgggg atcctcgagt gttgaatacc agtcggcccg gaatttggac
     5401 aggaacgtca aaaaatcggg cagatcgtgt tccgtcatct ggacaacacg ccggaaggcg
     5461 tcaacatcgt ccagagggag gtcaacgaga ttgcgttttc gtgccagatc cagcagggtc
     5521 tcgagtctga aggcaccttc gagatgccgg tgcagctcaa ccttgggaag ggccctgatg
     5581 atctcggcac tgggtccctt cggtttgttc ttatgcattc gtccggtcct gtatcgaaca
     5641 gcaagcgctg ttcctggtat ctgctcttat acttatcgat caaacacgga caaagcttga
     5701 acaaaacgtt tccccaaggg cgggcgggag gattggctta ttggccacgg gtcaccttga
     5761 cgaggcttcc gacgatatcg atgtcatagc cgtacacctt gaaggtcccg acggtgatcg
     5821 agacggggac gcgattggcg tcatcggtca tgcggacacg cacaccctgg ccgccgcgct
     5881 gttcgaagag caaggtcggg acagaccggc ctccaaccgg caggggatcg gctctccgga
     5941 ctgcgatgtt ggcatccttg accccttgct tgttgtccac gatgtaatcg gctgaaagca
     6001 tggtcccggg ttgtgcttgc tgtgcacgga catagtagac cagcgaaagg atgtccagtg
     6061 ttgcccgccg cagggggatc cggacgccat tggggttccg tttgtctctg tacagggcgg
     6121 tgcggttgtt ctggtcgata tcgatcgtca ccttgtccgt ccatgagcct tcctggatgt
     6181 ccttgtagac gcggatcggg aggagggtaa ccggatccat ccagacttcg atcacatcat
     6241 ggaggctgta gttgaaagcc gacttgatcg attcaaggga tctggtatcg gcgactgcat
     6301 gcaacaccct tcgaccgttg agcatgaccg gtccggtgaa cttcatgtcc tggtagccga
     6361 catgcagccc gaagaccttg atctggaacc ggatgacttc tcccggggag aacggaaagg
     6421 ccgccgcttt ggaaggactc aggatgagaa tgagcgtcat caagggtaca accagtgtga
     6481 ggagggagaa gcttcgggat ttcatgcaag tgactcctgt tcagcgaggg aagagtgtgc
     6541 tacatttgac tctacgagga tatataacaa taaagttccc aaggagttac tccctcatgt
     6601 cctgtctcca cccttttcga agtgttcgac caaggcccga tcttgcccgt gaggtggcca
     6661 gtcctccata cgacgtcctc tccagtgaag aggccagggc gatggccggt tccaatcccc
     6721 attcgttttt gcacatcaac aagtccgaga tcgatcttcc cgaggggacg gatctctatt
     6781 ccgacgcggt ctatgcccgc gccaaagaaa atttcgcacg gatgcgttcc gagggaatct
     6841 ttgtgcagga cgaaaagccc tgtctctatg tgtacaccct tgaagagggg agccaccgtc
     6901 agagcgggat tgccggatgc ttttcggccc aggaatatct ggacaaccat atccgcaagc
     6961 acgaacacac ccgcaaggac aaagaggaag accggacacg gcatatcgac gagaccaatg
     7021 cccagaccgg tgttgtgctc ctgaccttca aggcgcacgc ggatgtgaag agtcttgttg
     7081 agaaggtgac ggcgcgcaag ccactctacg atttcacggc cgaggacggg atccgccata
     7141 ttgtccacct gatcgatgat gccggcgaaa tctcggctct cgtcaagtat tttgagggaa
     7201 tcaaccccct ctacatcgct gacggccatc atcgtagtgc cggcgcttcc attgtgtatg
     7261 agcggcgcaa ggcggccaat cccgggcata ccgggaatga ggagtatgcc tacttcctcg
     7321 cggttgcctt ccccgacaac gagatgcgca ttctgcccta taaccgcgtg ctcgttgacc
     7381 tgaacggcat gaccaaagag ggcttttttg caaaactcgg cgaaaagttc gagattgcac
     7441 cgacacagga caaggctcca aagcaggaca aagaggtctc gatgtatatc ggtggcgagt
     7501 ggttcgggtt gcgctataat ggcagtcttc cggctgatcc attggcacgc cttgatgtgg
     7561 ccatcatgca gaacgaggtt ttgggaccac ttttcggaat cgaggatccc aaaaaagaca
     7621 aacggattga ctttgtcggt ggaacacgcg gaacggccga actcgaaaag ctggttgaca
     7681 gcgggaaatt tgccgtggcc ttctcgatgt ttccgacctc gatcggcgag ctgatggcgg
     7741 tggccgaaga aaaaaagatc atgccgccca agtcaacctg gttcgcaccg aaactccgtg
     7801 acgggcttct cgttcatgtc cttgaataag gccggcttga tacttgtggt gctcatggcc
     7861 tgcggctttt ccttcgccgc aggcgggctg gctgtctggc tcgccggtcc ggagcacgcc
     7921 ggtccggaga tgtacaggat tgcaggccgg tcctacgggc gggatgccgc tcttgccgag
     7981 tttgccgtga ccatgaagat gagcagccag ttttccccgg cccagaggac gcgattttcg
     8041 accaataccg ccttccgcaa tctctggctg cgcaaccgct atctctcgga tattctggcg
     8101 gcccacgccg gatcatctgg ttggcttgat tccgcagagt gtcaggcctg gttgcgcctg
     8161 gctgtccggg aggccgtccg gcaatacgtt ctggtgcggc atctcgatga tgtgactgtt
     8221 tccagcaatg aggtcatccg ttggtatgat gcgcatgcgc gaagcctgca gcaattaccc
     8281 ctcgatgagg ccatggtcta tgcgggtgaa cgggcacttc ttgaaaaacg gcaggatgcg
     8341 ctcatggagc ggaccctgga atacgccaag aaattggatg cgatattcaa aacgacgaat
     8401 ctctagggtg gaggtgcaga atggcttctt cggaaacggg acagatccgg gtcatctggc
     8461 gccccgtgtt tttgctgcct cgggaggcac gtcggatcct ggtggaggcg gccgatgccg
     8521 actctgacag acggtacggg atctggcgcc cctctctgct tgtgggaggc acggttgccc
     8581 tctttgccct gttgtttttg ggtggttccg gcctgaccgc atcgaatatc tttatcacgg
     8641 aaacagtccc ggccacaccc ctgcgggtct tgctgctcct gattgccggg tgtatcggga
     8701 tcctgttttt tttctttgcc atccggatcc tcgccaagag actcggtcat tcccggtatt
     8761 atctctatct cggggatgaa tggctggtcg agaggaatct ctggcatgtg agcatcattc
     8821 cggcttcgga tcttgtctac gcctggggac ggagggagtt ccgcacacgg ggcgccgatc
     8881 ttgagtatga gagtcttgtc tggcgtgacg gaaacgggag tctcaaggtc tatgatctcc
     8941 atgaccacta ctggcatggc gcaggccatg gacgccaggt cgcgtcaagc tggaatctca
     9001 tccgcaagca gtaccggatc ggcggcccgc cggccgggtt tggagcagga aagatgcatc
     9061 ggaaggaaac ttgatggaca atgctcttgt actggccagc ggcgggctgg attcgacgac
     9121 tgtcctggcg caggctgttg cggacgggtt tgcggtgacg accctctcgt tccggtatgg
     9181 ccaaaaacat gatattgaac tctcgcgggc gcgcgagatc tcctcacgat atggagcggt
     9241 gcgccatctt gagctcgaac tcgatccgcg cccctttgcc tcgtcggccc tgacctccgg
     9301 gggagagatt ccgttggatc gcacccacga gtcgatgggg gctgccatcg caccgaccta
     9361 tgtcccggcc cgcaacatgt tgtttttgac gatggccctg gccctggccg agggcctgcc
     9421 cgcgcgggac atctttatcg gggtcaacca tgtcgattat tcgggctacc cggattgtcg
     9481 cccggccttt atcgaggcgt ttgaaaaggc cgccaatctg gggacgagcg cggccgacgg
     9541 ggggcgtcct ttcaggatcc acgcgcccct gatgcagctt tcaaaagcgg gcatcatcca
     9601 cctggggatc tcgctcgggg tggactacag tcatacggtg agctgttatc tggcggacga
     9661 tcaagggcgg gcctgcggac actgcgacgc ctgtctcctg cgcaagcagg gctttgccga
     9721 ggccggtgtg cccgacccga cccgctatcg ccggtaagcc gggcctgtca acgagatcca
     9781 accatcagct tgctgtatga tgcatacgcc agtatctggc agtatgccgc tatcctgcag
     9841 cgcaatgctg atctggtgcc cgcctgtcgg taccaaaaca tgatactgca atctctatcc
     9901 gggataaaca ggatgatatt gcacaacagt aatcgcactc ccggagtcgc atgattattt
     9961 catgatttag tgatgcgact ccgggagtgg catgacgaga tcatgatatt cgcgggtgta
    10021 tcccggattg atgcgggcgg ccgtctgttc accataaaaa accggctcag gcaaccggac
    10081 ttgctctggc aaaatgctgt agtataacaa gatagagatt ctgtttcgcc atattgacaa
    10141 tcttaacttg tcaggttaag attgtcaata tggatatgca gatacagaga ctcttttcgg
    10201 accgtgtggc ggggctctgc cgttttttcc cggcgatcgt cctgaccggg gccagacaga
    10261 gtggcaaaac gaccctgctc aggcagcttt ttcccgggta tgcctatgtc tcgcttgatc
    10321 tgccggatgc ggcggccagg gctgaacaca gcccggagga attttttgcc cgctatcagg
    10381 ctcccctgat tgttgacgag gttcagtatg cgcccggtct ctttcgccat ctcaagacgg
    10441 tcattgatgc cgatcgcaca acgccgggcc ggtacatcct gaccgggagc cagctttttc
    10501 ccctgatgca ggaggttgcg gacagcctgg ccggacgctg cgccgtcctt gaactcgaaa
    10561 atctgaccct gagtgagatc cgtcaggccg aggtgcggga gtacaccctc gctgagcgga
    10621 ttgtgcgcgg ccagttcccc gaactctggc gccaacagga tctgccttca aacgagtatt
    10681 acgcttccta tctggcgacc tatctcgagc gtgacgtgcg ccagatcatg aatgtccgca
    10741 gcctgcggga ttttgagcgc tttttgcgga tattggcgtc acgctcgggc aatatgctca
    10801 acatgagtga tgtggccagg gatgtggggg tctcggtcaa ggccatcggt gactgggttt
    10861 cgatcctcga agcatccggg cagatcgccc tgcttgaacc atggtttacc aattttggaa
    10921 agcgccttgt caagacgccc aagctctatt ttcgtgacag cggcttgttg tgctttttgc
    10981 tcgggctcaa tccttccggg ctgcttgagt ccccgttttt gggggcggtc tgggagacct
    11041 ttgtctttgc tgaactgcgc aagggcgcgg gactggcgca gcacccggga aagttttggt
    11101 attaccggga ccagcgggcc agggaggtcg atttcttgta cgaaaacaac ggtcggctgg
    11161 attttatgga atgcaaatgg atggaacacc ccggaaaaaa ggaaacggcc atccttgcga
    11221 ccgtggccga cgaactcgac cgctcgagcc tgccctggaa gagcgccgcc cgccgcatcc
    11281 tgaccagcgg aagcacctcc tggcccctcg cgcccgggat cgaggcggtc ggctacccgg
    11341 agatcatggg gttggtgatg gagaggtgaa gcgtacggat gtctatgtcg tttggctgta
    11401 tcaaggtatt tgcggatcaa tcccggatta ctttccgcca gataaaaatt gtcaacatgc
    11461 gcaagaccgg gcttgaccgt gcgggcttgg cttaaaagag cattgtattc ggtttcatcg
    11521 gctgttgaaa atgggccaga caccatgctg cttgcgatga tgccgggagg aaagccgggg
    11581 tccccttcat atcctgatat atacacaggc ggtactgtaa agaatacagt catgaaattg
    11641 ccagagacga tgtttttcaa tagcccatcc caagcaacca gagcggaatg gcgcgggctg
    11701 ccgggtaatc gaggtcgtcg cggattacga aatccgcttc cttgcggctc tttgttgttc
    11761 cgccgatctc gatcgtcact ttttttcgtt ttccgccagt tgcgcgcgtg agcacgaaat
    11821 cgccggctcg ctcgtcgcgg ctcgcctcga cggtccagcc ggaggccgcg cagagacagg
    11881 ccaccagcgc ttcgcgggct gtcccgggat tgcccgcgag gacggggtag agtgccggat
    11941 ccgagaagaa gagcttggca ccgactgact gggtcttggt ctcgttttcc gcccggataa
    12001 cgctgaggat accgaccgat tccatgacat tgacaatctg atagagtttt tcggcgccta
    12061 tgccccagtc ggtgcagagg gatcggacct gcagccgcgg gatggcggag ccagcgaggg
    12121 ttccgacgat agccttcatc aggcgcagat taccgtcggt gatttgtggg aggaaaaaag
    12181 ggatgtcaaa gtagaggctc ttctcaagga cggccagaag cctatccttg aagtcgccct
    12241 cggcataaaa tggccgtgtg ccacactcgc ggtacctgcg gaaagcggca agaatatcag
    12301 catcgggcgc gaccggcagt tttttgccac ccgagaaggc atcgaaaata ggatactcgg
    12361 agcctgtttc cagaaagagg aactcgcgga aggagaggag cggcatgttg attcggacaa
    12421 agcgtcggga gaggtcgccg acaccatcgc gcaggacgag agccgatgaa tcgctgatcc
    12481 agatgccgtg tcggggaaat tcgtcgtaga gtgccttgac atggcggctc cagtcccggg
    12541 caaagtggac ctcatcgatg atgacaccct tgtatccggc ctgaaagatg gttttaacgg
    12601 tctcgtacag cgctgttcca gccagtgtcg gattgtcggc ggagaaatac agtagccgcg
    12661 agtttttggc atggtgtaac aggaacgtcg tcttgcccgt accccggggc ccgactagga
    12721 gcagggcgcg gggcaatgcg gaccgttccc gggtaaagtg acgaatccgt tccgggagtg
    12781 ctgccgtccg tcgttccatg gcagcttcca gttccgtaaa ttgttgtacc tcgttcatgt
    12841 ttcaagtata aacgactgat gttcctataa ca
//
LOCUS       DAOSHE010000009        12636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4174_length_12636_cov_5.518162, whole genome shotgun sequence.
ACCESSION   DAOSHE010000009 DAOSHE010000000
VERSION     DAOSHE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4174_length_12636_cov_5.518162"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="PLD82_00655"
     CDS             <1..1563
                     /locus_tag="PLD82_00655"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG63929.1"
                     /translation="QYTVALQEFSKVASEYRESPYAPKALEAIAWSYYGMKQYVRAVD
                     KYTELVTLYPDAPESEIALYRRGVILFNIKKSEEALNSFEDQLRRFPAGKLSEDAVFQ
                     TGWILYKVARYDQAARAFERYETAFKTGKRLTDAMFWRGMSLYRLKQYQEATVVFDTL
                     AKQHAGNEIAGRALVQSALCSLARKEMDNARQALQAVIRDTRYKSVQDEALYELARSW
                     LASEQPDMARVFINRLAEEFPQSQWTPEGVFRLGEYYFNKGLYDSARQHFNQMVASWA
                     GNPLAGDALYWIGWTYAREGRTAMALGIFRSFIEKYPENEYGADARMRMAAMFAKEGR
                     SAEAVDAYMEIIKRYRGTETAEMAAYEQALVFEQRREYDRAMRNFQVLAENALSTTVK
                     SRSYRHWGLWRIRHEKNEQEGLALLRKAAEADKGEAGGEARLELGRYFYGKGMQNRQL
                     YAQAYDECMKAVYLHANQKSIVVEALYLAADSLHKQGNKNGALSLLDRLIREYPDGEW
                     AVKARALKESMQ"
     gene            1560..3317
                     /locus_tag="PLD82_00660"
     CDS             1560..3317
                     /locus_tag="PLD82_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63930.1"
                     /translation="MKELLFIGILLGLFWFPFIGLEGTEQENLSLKRGGEEGTNSRMM
                     VPGSTADKALVLATNTRPGDNPASGGTSGKIRLPPTIINIEDLSEMRLERDGNLRLLL
                     PEIERARLRLDEPGRNPAFDHNGKKRKNTTSPGGDGDLDGSGRDGSGTRHVLSTFELT
                     YGFYDTLEALVSTGLEARDMAWQVTYRRKRDEGFDKDGKRLANSLRGSDSLVFDFSWN
                     RDAFDLSAGVVFSGGMLGLQGNTNFSQLRTIGLDARLQAGYIISTVTRMKLDFLLGHG
                     THVLDHPSARQEVENYSADAALEITMQWPRRLSLKTDIGLVLDESTDSGTGAHENSVT
                     AFSGGSRFGFSIGPVSMTVGGRLLAGESAWFSPGLDANWRLSPEFAFFGDIGREVFFL
                     RFRQSVLQEPFVSAVLPSLPVDRFRMAGGTRWQPSSSLSLVGTIVHASYSRYPGLVEN
                     ADGLYEYSFSDPDVTTVSAEIRFTPGSWLDLKAGWQQRLTSEEVPWLPDWEITTSMDV
                     LIPDSDTSLRAVLKIEGDRNLPGLGAYTLLDLRLQQAIGSTSSIIVDVRNLLGTKWRL
                     RPLYWQPGLTLHAGFSARF"
     gene            3414..4148
                     /locus_tag="PLD82_00665"
     CDS             3414..4148
                     /locus_tag="PLD82_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG63931.1"
                     /translation="MNAPFFLLAAVEKATNAVTNTAAMENVTAVKTSLADWIVQGGIM
                     MIPIILGSIVALAIIIERVIYLRKVRIETQRFMDKITRVVGANRIMEALVICDNTTGP
                     MPAIVKSAIALHDRSREEIRQAVEETASREVARLEKYLPALGTIGTVSPLLGLLGTVL
                     GMIKSSNHLAIAGTSNPVGLIGGISEALITTAAGLFVAIPVVVCHNWFTNQVNMLVLD
                     METKTTEILDMLSRTKSPQKGRGDKV"
     gene            4195..5412
                     /locus_tag="PLD82_00670"
     CDS             4195..5412
                     /locus_tag="PLD82_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG63932.1"
                     /translation="MTEYILLFSVLLVLSAFFSGSETAFFSLDMLKRSKLARDERPSA
                     MRASLLLRRPRELIMTVLIGNMLVNTALSSTASDLFGDDTGLAIVAITFTLLIFGEVT
                     PKRLGLRHNDRLVVFSASPLVFIKVLLSPIRNLFSWLMKPMLRRIGADAEPQMLTEDE
                     FKSAIKRSGASGALEKGESGLLANIITFSMLEARHIMTPRTSIQGLPVDAKMKEAFEM
                     VKAMPYSKIPLYKTNKDNIVGYLRAKDLLPYITGIRKQTTLKRLMRPVFRIPEGKLLS
                     DFLPEMQQNTSRLAVVVDEYGGTAGIVTLDDVLEEIMGQLLNENDKDESMYWSKTNRT
                     CVFRGTTPLAIFNKRMGTDFSSDDYQTLSGYVLELAGKIPQENDVFDDGVFRFRVMAL
                     KGKHVSQIEVVRK"
     gene            5409..6722
                     /locus_tag="PLD82_00675"
     CDS             5409..6722
                     /locus_tag="PLD82_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG63933.1"
                     /translation="MIEIWLLAGGILVCMALSAFFSGAESGMMLSDPIRLQHRADRGD
                     LRSRLILSMKQKPDLLIGTVLVGNNIVNVSAAVLTNRLLEHWVPSYAANFITTFGLTI
                     VMLMTSEIMPKLLFQSRPEELSRYVAPWLRVFGVILRPVLMLVNGISGVMVYLVGGKR
                     KDRQVLTREDITLLADVGMEQGAINARSYALLSSVLAFAKTTAREIMTPLVDVIALEE
                     DTSVEEVVKVIERAGFSRIPVYRKHAHTMIGYISAFDLKQSRRNASSAEYLRPAVFIP
                     ESKCIDSLLIEMRQKRLPMVFVVDEWGGTAGIITHEDIAEHVVGQILDKGEKMELEMQ
                     EVAPGEYLADGWTDVDKVQQVLRIRIEKKGFETIGGFLEHLMQRIPSVSESVLYEGYR
                     FHVEEADSTRIVRVRIKRTGSRKRGANEQAVPKKPVFPEQNGKKE"
     gene            6719..8713
                     /locus_tag="PLD82_00680"
     CDS             6719..8713
                     /locus_tag="PLD82_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOG63934.1"
                     /translation="MKKNAQKQTVLDTISGSDAGSTLEQIIGEKTDIAGVFAELSRLL
                     REKKIVYQGQHFHLKEKTPKEEFKDSFNDLDRVLQKYSLPAEFPRPVLDQARRCGEAG
                     KRELPKRKKLDEWIITIDSESAKDLDDAVSVRQVRNGWELGVHIADVSFYVPRGSLLD
                     KDAFARGTSVYLNRHVVPMFPHELSDDLCSLNANSQKLALSAFLSIDTTGKVTAARFE
                     KTVIQVARRFSYNEVEAILRGKKDADAHHLKVMADLAKILRIRRHEQGGLEFEVPEVR
                     ISLDDSGDAVGVTVPVRNEAEMMIEDFMLAANEQVASFLSKKGASIFRIHASPDQEKL
                     QEFVKFARGIGLGLRVPADNTPRAMQHLLEEVRSNPNAGVLSSLLLRTMQKAVYSTDN
                     PGHFGLAFADYTHFTSPIRRYPDLVVHRLLSAAIEGKKAYTQKELERIAEQATKMEQN
                     AVDAEREYTRIKGARFLGKKIGETFEGKVTSVTSWGMFITLLQWGLDGLLHISELRDD
                     YYRLDTWGHTLVGERTRRVYSIGSIIKVKIKSVNAEKGFVDLVLEESKQAGSKKGESS
                     RNRQSGQNTRERTRSTEKGSGSGQKNAQVSRPGDPNEQKGPMERQANRNRSRGRSAGP
                     VSPVEKSAKFRENPLAQPEQAKSGANAETGKKKGWLFWKK"
     gene            8767..9111
                     /locus_tag="PLD82_00685"
     CDS             8767..9111
                     /locus_tag="PLD82_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOG63935.1"
                     /translation="MKILVILLVGLVVFACGKTDESKAVDQEKSGVSQSGTVTNTISS
                     ADLAKNDGKNGRPAWVAYKGKVFDVSAVPAWKTGVHKGHAAGQDITSQIASSPHGDKV
                     FSKLQPIGILGK"
     gene            9207..9602
                     /locus_tag="PLD82_00690"
     CDS             9207..9602
                     /locus_tag="PLD82_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG63936.1"
                     /translation="MKFKRRLQYRVGIDMTPMIDSVFLLLIFFMITSSIIKDQGIQVA
                     LPHAKSAESQPDRDLVISISREGAIFLNDAKVAKKDLYQKLRKVYKEAKRDFLIIRAD
                     EVIEYGVLVEVMDIARLAGIYNVSLSTKR"
     gene            9632..10480
                     /locus_tag="PLD82_00695"
     CDS             9632..10480
                     /locus_tag="PLD82_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG63937.1"
                     /translation="MDRNQYSLRFDRMVVMSIIAHCLLLLFVSLLWGGSAVPAIKTVQ
                     LVIESRPVAREVIAEVKRESAPGRVKRDAVSTGSAEESLESLLERSTFPHQPVAERDA
                     PNRKREDDFDYGSLRRDQESPDGRLDRNPDSKDMQAHRPSITDRDTRTKEGTRETFGR
                     DDATRQSGKNGNPDIKGSDFGKQGDRGAGVTGLVNMRGRSLLYRPTVVLPERFSRQGI
                     SYSVQVEITVSEEGIVSRAAVLEPSGDSGLDLLLEQRARMYRFEAASPGTGVQTGTIV
                     FTIQPR"
     gene            10503..>12636
                     /locus_tag="PLD82_00700"
     CDS             10503..>12636
                     /locus_tag="PLD82_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63938.1"
                     /translation="MVFPSVMVGSILFFLFSLSMLVNYALGNLLVSIEDDLVGGLERD
                     HDIRIDYDSISVASFDTVVMRGLKIRHGDTNKQAVLASLASAEIRVRIFPLLFGTKDA
                     LETIASVNIEKPELALYRDGSLLDKFGANKTKKDPSGKTVATPKFDVNLNVRNGQIRV
                     YSSDSRAAIPPAAPVAELDFSDIKMELNQNRGSFGIKTGLAVLPGQDLAAGKVDISGI
                     LNLDTGSVDALVTTSDAWIAGLGLPELRFFVTREEGQIRVDSSTFGQPVSIDGRWKEG
                     QDGSFILTLDKSLKSFENFVTKTSLGVLGKMFFTKGERYTPSGTISLDIGADGARMAG
                     SLAIEDSQGKPVFRLAATGNQEILSVERLELARGEHLISVSGYWDWKGIPEIAARVRG
                     FEVESASVDANISVREKNNGLVSVGIEGLGINGQYIGSLNALVDGKSPAVVIDGFDGA
                     LRGRIDGGQGDTVVTLDCRGFKAGAIAGALAGMDFISGWNLTGNITLKSIESGVTLSG
                     DTVIQTGDYGELGAKFDFTGNEIKLKKLSSDWFTISGTAHREYDGASGRFTVLYNGRE
                     WPLVAHMKQTGGMTRAGFEIEGLFSGQADISADGLRASFAVHDLDLHPVGLEAMLAGE
                     GTIVQKGGMLSSGGKVLLQMKDPEIPSVSIEFQGAGKRFDVTRFVLERQGHALIGGGT
                     AWIDEQGASRLSMAFANGIAFQASSLAGG"
BASE COUNT         3067 a         2956 c         3706 g         2907 t
ORIGIN      
        1 cagtacacgg tcgccctcca ggaattttcc aaagtggcga gcgagtatcg ggagagtccg
       61 tatgccccga aggcacttga agcgattgcc tggagctact atggcatgaa acaatacgtc
      121 agggccgttg acaagtatac cgagttggtg actctctatc cggatgctcc cgaatcggag
      181 atcgctctgt accgtcgcgg cgtgatcctg ttcaacataa aaaagagcga agaggccctc
      241 aattcattcg aggatcaatt gcgccgcttt ccggctggaa aactctccga agatgccgtc
      301 ttccagacag gctggatcct ctataaagtt gcaagatacg accaggctgc ccgggcgttt
      361 gagcggtatg agaccgcatt caaaaccgga aagaggctga ccgatgccat gttttggcgc
      421 gggatgtcat tgtacagatt gaagcagtat caggaagcca cggtcgtttt tgacacgctg
      481 gcaaagcaac atgcaggaaa cgaaattgcc ggtcgtgccc ttgtccagtc cgcgctttgt
      541 tcgcttgcac ggaaggagat ggacaacgcg cgccaggcac ttcaggctgt catccgtgac
      601 acacggtaca agtcagtcca ggacgaggct ctctacgagc tggcgagaag ctggctggcg
      661 tccgaacagc ccgacatggc acgtgttttc atcaacaggc ttgcagagga gtttccgcag
      721 agccaatgga caccggaggg cgtctttcgc ctcggggagt attatttcaa taaaggattg
      781 tatgacagtg cgcgccagca tttcaaccag atggtggcaa gctgggccgg aaaccccttg
      841 gcgggtgatg ctctctactg gatcgggtgg acctatgccc gcgaaggcag aacggcgatg
      901 gcgctcggca ttttccgttc atttatcgag aagtatcccg agaacgagta tggtgccgat
      961 gcgcggatga ggatggctgc gatgtttgcc aaggaggggc gatcggccga agcagttgat
     1021 gcatatatgg aaatcatcaa acggtaccgc gggactgaaa cagcggaaat ggcggcctat
     1081 gagcaggccc tggtatttga gcagcgacgg gagtatgacc gtgccatgcg gaattttcag
     1141 gtcttggcgg agaacgcatt gtcaacaaca gtgaaaagcc gatcttaccg gcattggggt
     1201 ctctggcgaa tccgtcatga aaaaaatgag caggaaggac tcgctttgct caggaaagcc
     1261 gccgaggctg acaagggaga agctggagga gaagccaggc tcgagctcgg aaggtatttt
     1321 tacgggaagg gtatgcaaaa cagacaactc tacgcgcaag cgtatgatga atgcatgaaa
     1381 gccgtgtatc ttcacgccaa ccaaaaatcg attgtcgtag aagcgctcta tctggctgcc
     1441 gactcgctgc acaaacaggg aaacaaaaac ggagctcttt cgctccttga ccggttgatt
     1501 cgggaatatc cggatgggga atgggctgtc aaggcccggg cgctcaagga gtccatgcaa
     1561 tgaaagaatt gctcttcatc gggatacttc tcggcctgtt ctggtttcct ttcatcgggt
     1621 tggaaggtac ggaacaggaa aacctttcac tcaaaagggg tggcgaagag ggcacgaatt
     1681 cccggatgat ggttccgggg agcaccgctg acaaggcact ggtcctggca accaatacga
     1741 ggcctggaga caacccggcc agtggcggga caagcggcaa gatccggctt ccaccgacga
     1801 tcatcaatat tgaggatctc tccgagatgc gtctcgaaag ggacgggaat ctcaggttgc
     1861 ttctccctga aatcgaacga gccagactca ggcttgacga acccggaagg aatccggcgt
     1921 ttgaccacaa cggcaaaaaa aggaaaaaca ccacatcccc cggaggggat ggggatctgg
     1981 acggaagcgg tcgagatggt tccgggacac ggcatgtttt gtccacattt gaactcacgt
     2041 acgggtttta tgatacgctt gaggcgcttg tctcgaccgg cttggaagcc agggatatgg
     2101 catggcaggt cacataccga cgaaaacggg atgaaggatt tgacaaggac ggaaaacggt
     2161 tggcgaactc gttgcgaggg agcgattcgc tcgtctttga tttttcctgg aacagggatg
     2221 cgtttgatct ttcggcgggc gtcgtgtttt cgggaggaat gctcggattg caagggaata
     2281 ccaatttttc ccagcttcgc acgatcgggt tggatgcgcg actgcaggcg ggatacatca
     2341 tttcgacggt caccaggatg aagcttgatt ttttgcttgg tcacgggacg catgttctgg
     2401 atcatccatc agcgagacag gaggtcgaaa actattcggc tgatgccgct ctggagatca
     2461 ccatgcaatg gccgagacgt ttgagtctga agacggatat cgggttggtg ttagacgaga
     2521 gtaccgattc cggaaccggg gctcatgaaa acagtgttac agcgttttca ggtggttcac
     2581 ggttcggatt ttcgattgga ccggtgtcga tgaccgtcgg gggccgtttg ttggccggtg
     2641 aatcggcgtg gttctcgccc gggcttgatg cgaattggag actttcaccc gagtttgcgt
     2701 ttttcgggga tatcgggcgt gaagtttttt tcctgagatt cagacagagc gttttgcagg
     2761 aaccgtttgt ttcagctgtc ttgccatcac tcccggtcga cagattcaga atggcgggag
     2821 ggacccgttg gcaaccgtcc tccagcctct ctcttgtcgg gacaatcgtg catgcatcct
     2881 attcaaggta tcctggactg gttgaaaacg ctgatggttt gtatgaatac agtttcagtg
     2941 atccggatgt cacgactgtt tccgccgaga tacggttcac tccggggagc tggctcgatt
     3001 tgaaggccgg ttggcaacag cggctgacgt ccgaggaagt tccgtggttg ccagattggg
     3061 aaatcacgac atcgatggac gtgcttatcc cggattccga tacatccctt cgggcggtat
     3121 tgaagatcga aggcgaccgg aatttgccgg gattgggtgc ctataccctg ctggatttga
     3181 ggcttcagca ggccatcggt tcaacatctt ccatcatcgt tgacgtccgg aatctgcttg
     3241 gaacgaaatg gcgtctgcga cccctttatt ggcagccggg tttgacactt catgccggat
     3301 tctcggcgcg gttctgaaat tggcaggaga agtctcatcc atgtgcgcgg ctcttgtggt
     3361 gcgtgccgga atagtatatt ctcatacgag agagtcgaaa gggaaggagc cccatgaacg
     3421 ccccattttt tctgcttgca gcagttgaaa aagcgacaaa cgcagtcacg aatacagccg
     3481 caatggagaa tgtgacagcc gtcaagacat cgttggctga ctggattgtc cagggtggaa
     3541 tcatgatgat tcccattatc cttggatcga ttgttgcttt ggcgattatc attgaacgtg
     3601 tgatttatct ccgcaaggtc cggattgaga cacagcgatt catggacaag atcactcgcg
     3661 ttgtcggagc caatcgcatc atggaagccc tcgtgatctg cgacaacacg accggtccca
     3721 tgcctgccat tgtcaaatcc gcgatagcat tgcatgaccg aagccgtgag gaaatccggc
     3781 aagccgtcga agagacagcc agccgtgaag tggccaggct tgaaaaatat ttgcctgcac
     3841 tgggtaccat aggaaccgtc agcccgttgc tgggactttt gggcactgtt cttggtatga
     3901 taaaatcctc aaatcacttg gccattgccg gaacatccaa cccggtcggt ttgattggcg
     3961 gaatctcgga agccctgatc acaaccgcgg ccggtctgtt tgttgccatt ccggttgttg
     4021 tctgtcacaa ctggtttacc aaccaggtga atatgctggt cctcgatatg gaaaccaaga
     4081 cgaccgagat ccttgacatg ctttcacgaa ccaaatcacc ccaaaaaggt cgcggggaca
     4141 aggtgtgatg gaaagcggta ctattcggtc cgaccggacc atgatggtgg cagaatgacg
     4201 gaatacattc ttctgttttc cgtcctgctg gttttgtcag cctttttctc cggatcagaa
     4261 acagcctttt tttcgttgga catgctcaag cggtcaaagc ttgccaggga cgaacggcct
     4321 tccgcaatgc gggcgtcgct cctcttgcgg cggccgcggg agctgatcat gactgtcctg
     4381 atcgggaaca tgcttgtgaa tacggcgctc tcctcgacag catccgatct tttcggggat
     4441 gataccgggc tagcaatcgt ggcgatcact tttaccctcc tgatttttgg cgaggttacg
     4501 cccaagagac tcggtctgcg gcacaacgac agattggttg tattcagtgc atcgccgctg
     4561 gtgttcatca aggtgcttct ttcacccatc aggaatctct tttcctggct gatgaaaccg
     4621 atgttgagac gaatcggagc cgatgcggaa ccacagatgt tgaccgagga cgagttcaag
     4681 agcgcgatca agcgcagtgg ggccagtggc gctcttgaaa aaggcgagag tggattactg
     4741 gccaatatca taacattttc gatgctcgag gcgcggcata tcatgacgcc ccggaccagc
     4801 attcagggtt tgccggtgga cgccaaaatg aaagaagcct tcgagatggt caaggcgatg
     4861 ccctattcaa aaataccttt gtataaaacg aacaaggaca atattgtcgg atatctcagg
     4921 gcaaaggatc ttcttccata tataaccggg atcaggaagc agacaacgct caagcgattg
     4981 atgcggccgg ttttcaggat acccgagggg aaactcctgt ccgacttttt acccgagatg
     5041 cagcagaata ccagccggtt ggccgtggtt gttgatgaat atggcggaac ggcaggaatc
     5101 gtcacccttg atgatgtcct tgaggagatc atggggcagc tgctcaatga aaatgacaaa
     5161 gatgaaagca tgtactggtc caagacaaac aggacatgtg tctttcgcgg aacaaccccg
     5221 ttggcgattt tcaataaacg gatgggcacg gatttctcga gcgatgatta ccagaccctt
     5281 tcgggatacg ttcttgagct tgcggggaaa atcccgcagg agaatgatgt ttttgatgac
     5341 ggggtattcc gttttcgtgt catggcactc aaggggaaac atgtcagcca gattgaggtg
     5401 gtccggaaat gatcgaaatc tggcttctcg cgggcgggat tctggtatgc atggctctgt
     5461 cggccttttt ctcgggtgcc gagtccggga tgatgctctc tgatcccatt cggcttcagc
     5521 accgggcgga caggggtgat ctgcggtcac ggctgatcct gtccatgaaa caaaaaccgg
     5581 atttgttgat cggaactgtc ctggtcggga acaatattgt caatgtttca gcggctgtgt
     5641 tgaccaacag actcctcgaa cattgggttc cgtcatatgc agccaatttt atcacgacgt
     5701 tcgggttgac aatagtcatg ctgatgactt ctgaaatcat gcccaaactc ctgtttcaaa
     5761 gccggccgga agagctctcg cggtatgtgg ccccatggct ccgtgttttt ggggtgatcc
     5821 tgagaccggt cctcatgctt gtcaacggga tatcgggagt gatggtctat ctcgtcggag
     5881 gtaagagaaa agaccgtcag gttctgacac gtgaggatat cacgctcttg gccgatgtgg
     5941 ggatggaaca gggcgccatc aacgcccgtt cctacgcgtt gctcagttca gtcctggcat
     6001 ttgccaagac cacggcccgt gagatcatga caccactggt cgatgtgatt gcgctcgagg
     6061 aagatacgtc tgttgaagag gttgtgaagg tgatcgagcg cgccgggttt tcccggatac
     6121 ctgtgtaccg gaaacatgcg catacgatga tcggctatat ctccgcattc gatctcaagc
     6181 agagcaggcg gaatgcgagc agtgccgagt atctccgtcc ggcggtcttc atacccgaat
     6241 ccaagtgcat tgacagcctg ttgatcgaga tgcggcagaa gcggcttccc atggtatttg
     6301 tcgtcgatga atggggtggg accgcgggca tcatcaccca tgaggacatt gcggaacatg
     6361 tagtcgggca gattctggac aagggcgaga agatggagct ggagatgcag gaggtggctc
     6421 cgggcgagta tctggccgac ggttggacag atgtcgacaa ggtccagcag gtgttacgga
     6481 tacggatcga aaagaagggg tttgaaacaa ttggaggatt tttggaacac ctgatgcaga
     6541 ggattccctc cgtcagcgag agtgttctct atgaggggta tcgcttccac gtcgaggagg
     6601 cagacagcac ccgaatagtc cgtgttcgta tcaaacgaac aggatcacgg aaacggggag
     6661 cgaacgaaca agcagtgccc aaaaagccgg tgttcccgga gcagaacgga aaaaaagaat
     6721 gaaaaagaat gcacaaaaac agacggttct tgacacgatt tctggatcgg atgcaggcag
     6781 cacattggag cagattatcg gcgaaaagac tgatatcgcc ggggtttttg ctgaactttc
     6841 gcgacttctt cgggagaaaa aaatcgtcta ccaggggcag cattttcacc tgaaggaaaa
     6901 aacaccaaaa gaggaattta aggacagctt taacgatctt gaccgtgttc ttcaaaagta
     6961 ttctcttccg gccgagtttc cgcgaccagt ccttgaccag gcacgtcgtt gcggggaggc
     7021 gggcaaacgc gaactgccaa aaagaaagaa actggatgaa tggatcatca ctattgattc
     7081 cgaaagcgcc aaagatctcg atgatgccgt ttcggtccgg caggtccgga atggatggga
     7141 gctcggtgtg catatcgcgg atgtttcatt ttatgtcccg aggggtagcc ttctcgacaa
     7201 ggatgcattt gcacggggaa cgagcgtgta tctcaaccgt catgtggtac cgatgtttcc
     7261 gcatgaattg agtgatgatc tgtgcagcct gaatgcaaac agccaaaaat tggcactctc
     7321 ggcatttctg tcgatagaca cgaccgggaa agtgacggca gccagatttg aaaaaacggt
     7381 catccaggtt gcaagaaggt tttcatacaa tgaggttgag gcaatccttc gcgggaagaa
     7441 ggatgccgac gcgcatcatc tcaaggtcat ggccgatctg gccaaaatcc tcaggatcag
     7501 acggcatgaa cagggcgggc tggaattcga agtacctgaa gtgagaatca gcctggatga
     7561 ttctggtgat gccgttgggg tcacggttcc ggtcagaaat gaagccgaga tgatgatcga
     7621 agacttcatg ctggctgcca acgaacaggt ggcaagtttc ctttcgaaaa aaggggcatc
     7681 gatcttcagg atccatgcca gccccgacca ggaaaaactg caggaatttg tcaagtttgc
     7741 acgagggatc gggctgggat tgcgggttcc ggccgacaat acaccgcgag ccatgcagca
     7801 tcttctcgag gaagtgcgtt ctaatccgaa cgccggggtg ttgagcagtc tgcttttgcg
     7861 gacaatgcag aaagccgtgt acagcacgga caacccgggc catttcggtc ttgcatttgc
     7921 cgattatacg cattttactt cgccgatcag gcgctatccc gatctggttg tccaccggct
     7981 gttgtcagcg gccatcgaag ggaaaaaggc gtacacccaa aaggagctgg aacggattgc
     8041 cgaacaggcg acaaagatgg aacagaatgc cgttgatgcc gagcgggagt atacgcgcat
     8101 caagggcgct cgattcctcg ggaaaaagat cggcgagact tttgaaggca aggtgacatc
     8161 tgtcacttcc tggggcatgt tcatcaccct cctgcaatgg ggtctggacg gactactcca
     8221 tatctccgag ttgcgggatg attattaccg gcttgacaca tgggggcata ccttggtcgg
     8281 agagcggacc cgacgggtgt acagcattgg gagcatcatc aaggtcaaaa tcaaatccgt
     8341 gaatgcggag aagggttttg ttgacctggt tcttgaagaa agcaagcagg caggatcaaa
     8401 aaaaggcgag tcttcgagaa acagacagtc tggccagaat acccgtgaga ggaccagaag
     8461 cacggaaaag ggttccggat cgggtcagaa aaatgcccaa gtctcccgtc ccggtgatcc
     8521 aaatgaacag aaaggcccga tggaacgaca ggcaaacagg aatcgttccc gggggcgctc
     8581 tgccggacca gtttctccgg ttgaaaaatc tgcaaagttc cgggaaaatc cgcttgccca
     8641 gccggagcag gcgaagtcag gggccaacgc ggagaccgga aagaaaaagg gatggttatt
     8701 ctggaaaaaa taagtgatac tcttggtacc cgtgcttgca cgaatcatga aatgactgga
     8761 ggttccatga aaattttggt tattctgttg gtgggtttgg tggtctttgc ctgcgggaaa
     8821 acggatgaat caaaggctgt cgaccaggaa aagagcgggg tgagtcagtc tggcactgtg
     8881 acaaatacga tatcttcggc tgatttggcc aagaatgacg ggaaaaatgg tcgtccggca
     8941 tgggttgcct acaagggcaa ggtgttcgac gtctccgctg tgccggcctg gaagaccggt
     9001 gtccataagg gtcacgctgc cggtcaggat atcaccagtc agattgcatc gtccccgcac
     9061 ggggacaagg ttttctccaa gcttcaaccg atcggaatcc tcggaaaata atcggagttt
     9121 ccatctgatc aagaccgccc cccggcggtt ttttcatccc tgagtgcagt atatttatag
     9181 aagagagatg tagtattggg ggaaacatga aattcaaacg taggctgcag tatcgtgtgg
     9241 gcattgacat gactcccatg atcgattctg tttttctctt gttgattttt ttcatgatta
     9301 cgtcatcaat catcaaagat cagggcatac aggtggcttt accccacgca aaatcggcag
     9361 agtcccaacc ggatcgtgat cttgtcatct ccatcagtcg tgagggggct atttttctca
     9421 atgatgccaa ggtagccaaa aaagatctct atcaaaaact ccgaaaagtc tataaagagg
     9481 ccaaacggga ttttttgatc atccgggcag atgaggtcat tgaatacggt gtccttgttg
     9541 aagtgatgga tattgcgcga ctggccggta tctataatgt atcgctctcg accaagcgtt
     9601 agttggggga tgggcggaga ggagctggaa aatggatcgc aaccagtata gtctgcggtt
     9661 tgaccgcatg gtcgttatgt ccattattgc acactgtctt cttctcctgt ttgtttcgtt
     9721 gctctggggc gggagtgcag tccctgctat caagacggtc cagctcgtca tcgaatcccg
     9781 tcccgtcgcg cgggaggtga ttgccgaagt caaacgcgag agcgcacccg gtagggtcaa
     9841 acgtgatgcg gtcagtacag gctctgccga ggaatcgctg gagagtctgc tggagagatc
     9901 cacgtttccc catcaaccgg ttgccgaaag ggatgctccg aaccgaaaac gagaagatga
     9961 tttcgattat gggtcccttc gtcgtgatca ggaatctccg gatgggcgat tggacaggaa
    10021 tccggattcc aaggatatgc aggctcaccg accttcaata accgatcggg atacacgaac
    10081 caaagaaggg acgagagaga ccttcggtag ggatgacgca acccgccaat ccgggaaaaa
    10141 cggaaatccc gatatcaaag gctccgattt cggaaaacag ggagaccgcg gagccggagt
    10201 gactggcttg gtaaacatgc gagggcggag tttgctgtat cggccgacag tagtattgcc
    10261 cgaacgtttc agcaggcagg gaatctccta ttctgttcaa gtcgagatta ctgtgtcgga
    10321 ggaaggtatt gtcagccgcg cggctgttct cgaaccaagc ggggattctg gtctggatct
    10381 cctgctcgag caacgggcac gaatgtaccg cttcgaagct gcctctcctg gaacaggagt
    10441 gcagaccggc acgatcgtct ttaccataca accgaggtaa tcccgtgaaa ttccggagag
    10501 gtgtggtatt tccatccgtg atggtcggga gtatcctgtt ttttcttttt tccctttcaa
    10561 tgcttgtcaa ttacgcactt ggcaatcttc ttgtctcgat cgaagatgat ctggtgggag
    10621 gattggagcg ggatcacgat atccggattg actacgactc aatctcagtc gcgtcatttg
    10681 atacggttgt catgcgcggc ttgaaaatcc ggcatggcga tacaaacaag caggcagttc
    10741 tggccagtct tgccagtgcg gagatcaggg tacggatctt cccgttgttg ttcggaacga
    10801 aggatgcgct ggagacgatc gcttcggtga atatcgaaaa gcctgagctg gcactgtatc
    10861 gggatgggtc ccttctcgac aagttcgggg caaacaagac aaaaaaggac ccatccggga
    10921 agaccgtcgc gacgcccaag tttgatgtca acctgaatgt caggaatggc cagattcgtg
    10981 tctattcttc ggattcccgt gccgcgatac caccggctgc acctgtggct gaactggatt
    11041 tttccgatat caaaatggaa ttgaaccaga acagggggag tttcgggatc aaaaccggct
    11101 tggcggttct tcccgggcag gatctggccg ccggaaaagt cgatatctct gggatcctga
    11161 atcttgatac gggctcggtt gacgcattgg tgacaaccag cgatgcctgg attgccgggc
    11221 ttggtcttcc agagttgcga ttttttgtca ctcgtgaaga aggccagatc agggtggact
    11281 cttccacatt cgggcaacct gtctcgattg atggtcgctg gaaggaaggg caggatggaa
    11341 gtttcattct gaccttggat aaaagtctga agtcatttga aaattttgtg accaaaacat
    11401 cattgggcgt tttgggaaaa atgtttttca caaagggcga gagatacaca ccatcaggaa
    11461 caatttccct ggacattgga gcagatggtg cacggatggc cggttctttg gcgattgagg
    11521 acagtcaggg aaaaccggtt ttccggctgg cagcaaccgg gaatcaagag attctctctg
    11581 tagagcgtct cgagttggcg agaggcgagc atctgatcag tgtttccgga tactgggact
    11641 ggaaggggat tcccgaaatt gcagccaggg tccgcgggtt cgaggtggaa tccgcaagcg
    11701 tggatgcgaa tatctcagtc cgcgagaaaa ataacggact tgtttccgta ggaatcgaag
    11761 gacttggtat caatggtcaa tacatcggtt cgctgaatgc gcttgtcgac gggaaaagcc
    11821 cggctgttgt cattgatgga tttgacggtg cacttcgtgg caggattgac gggggtcagg
    11881 gtgatactgt tgtgacactg gactgtcgtg gcttcaaggc aggagcgatt gccggagcac
    11941 tcgcgggaat ggacttcatc tccggatgga atcttacagg caatattaca ctcaaaagca
    12001 tcgaatcagg agtgacactc tccggtgata ccgtgatcca aaccggggac tatggagagc
    12061 taggagcaaa atttgacttt acaggaaatg aaatcaagct gaaaaaattg tccagcgact
    12121 ggttcacgat cagcgggaca gcccatcggg agtacgatgg cgcaagcggg agattcaccg
    12181 ttctgtacaa tggcagggaa tggcccctgg tggcacacat gaagcagacc ggaggcatga
    12241 cccgggccgg atttgaaatt gaaggacttt tttccgggca ggccgacatt tccgcagatg
    12301 gtctccgggc atcgtttgcc gttcatgatc ttgatcttca tccggtcggt cttgaggcca
    12361 tgcttgcagg cgagggaacg atagtccaga agggtggcat gctttccagt ggtggcaagg
    12421 tgttgctgca aatgaaggat ccggaaatac cgtcagtgtc gatcgagttt caaggagcag
    12481 gaaagcggtt tgacgtcaca cgctttgttc ttgagcgaca ggggcatgct ctcattggag
    12541 gtggaacagc ctggattgat gaacaggggg cctcccgtct ctcgatggct tttgcaaatg
    12601 gtatagcatt tcaggccagt tctctggctg gtggat
//
LOCUS       DAOSHE010000010        12516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4225_length_12516_cov_5.481101, whole genome shotgun sequence.
ACCESSION   DAOSHE010000010 DAOSHE010000000
VERSION     DAOSHE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4225_length_12516_cov_5.481101"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLD82_00705"
     CDS             complement(<1..1231)
                     /locus_tag="PLD82_00705"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOG63939.1"
                     /translation="MSYDEESIRKAKDKGIFNDSFDPLALGLSEDEKKRMAKGFEGAQ
                     RDLMNKAEASDNKSGKKRGKQGQQDVKKAGFFERLVRFFVMLFTGLSPADYEKKKRIK
                     ELKLAIKKLKPAVYNFQTEAITAEFPGLIHDFFKLVWPYRIVFEEVFGETDPESHLDF
                     QLFYVRTLVPTITDEEIFRFTEDGIKEYVKGDRDSMVRKRIDEMTDIFIRKIDEETRR
                     RLNRTFADFINVKELLSYNFFSFLKRFQSGYSVDDPNPDFHDIRPDGTGEMLKDLESI
                     LLAVNTKALPSVLGLAAAYFQENGSKTADEETVAKTLVHFQKMAEGGYRMIPASLSKI
                     VNGQQLCLLIRYITKNLEYEAHIYPRGTNFFEEFGRGLVAAMEARVSRVLARRKQEEV
                     DAKLKELFETQEQMPDYL"
     gene            complement(1246..2079)
                     /locus_tag="PLD82_00710"
     CDS             complement(1246..2079)
                     /locus_tag="PLD82_00710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG63940.1"
                     /translation="MAIAALFMIYIYLGKESFMKGSGYGLPALISGDVAAGERRIELD
                     RLKLETKKQPGTAETVQKIQKLDAMVSKDSAVAGERGRSIHYLRYAGWFLSTFFILFV
                     LPFVFIAVHPRLRLKDYGLGLGDWLFSLKIFSAFAVVMALAVAAIKVFHIEGFLRYYP
                     MFAVKGITGHQPDFVFWFIILETCFFLYFVGWEFFFRGLLIFPLSRRIGSLAALVGLV
                     PFAIMHAGKPVAEAFGSIIAAWVLGILVLRARSFWVCPVLHFAIAFIMDLMAALSRNL
                     F"
     gene            complement(2164..3027)
                     /locus_tag="PLD82_00715"
     CDS             complement(2164..3027)
                     /locus_tag="PLD82_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOG63941.1"
                     /translation="MKKLLALVLVLVVTGLPLSAADTAGIPMLFYGEASWYGDVYHGR
                     TTASGETYDMTMLTAAHRTLPFGTLVRVTNLENDKSVTVRINDRGPFAKERVIDVSRA
                     AASLLDFRTKGTAKVEIRVVQLGDGKMQAATTAASAVPALTRNEDTRNSAVTVASNAN
                     QNPVYPADQGTNGGAVETPRGTGTTLTDGPRIIRPEDVQHKNDDNATVKPVETGKIVI
                     QAGAFLSLDNAESLLRSLQAMGLPVFIRQFSGERLHRVWVGPFAAKADADQVLEKVRS
                     KAEQAFIRNRN"
     gene            3116..3655
                     /locus_tag="PLD82_00720"
     CDS             3116..3655
                     /locus_tag="PLD82_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG63942.1"
                     /translation="MDKQSLYRFGNFFPKVDPSVYIAPGARIVGDVTLGENTSIWWNT
                     VLRADLAPIIVGPRSNIQDNATLHVGRNQACILGTDVLVGHGAICHACTIDDGALIGM
                     GAIILSGVTIGHHALIAAGSLVSERTEIPPQTLWAGNPARCIKSLGPGMLERMREGSA
                     LYLSLAALSRDDGPHRDTD"
     gene            complement(3656..4417)
                     /locus_tag="PLD82_00725"
     CDS             complement(3656..4417)
                     /locus_tag="PLD82_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG63943.1"
                     /translation="MLLDEVTGGLDALLERKRIADWSNNGLQIEGCDQIRRIAFAVDA
                     GLKTIQLAVEAKADLLVVHHGLFWNQPLMITGTHRQRVAACIESNLSLYAAHLPLDVH
                     PLYGNNAGILEDAGFTITGNFAEERGTKVGSFGQSQEGRSFESVIGALEKTLGSGPTR
                     ILNECSPDTVFHSAGAITGSGLKYAEEAARLGAELFISGEGSHASYHQIVECGIVCLL
                     YGHYLSETVGIKRLMKHCATSWPVETCFLDAPTGF"
     gene            complement(4417..5355)
                     /locus_tag="PLD82_00730"
     CDS             complement(4417..5355)
                     /locus_tag="PLD82_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOG63944.1"
                     /translation="MLLQKRMKIVVFVILSLALLVPMIVHALSDITVINFGNYEKRIT
                     AEFQSISTNSKPKNPDGSPNYILTPSVMAISNWIVEVRSSGVRIPNIVKSQCLSVFSK
                     LQNQNVLGVRINFPETRQNDRAYIRPQFEFHVYDKKGNFSNISNGIVTNVGLVKDVSV
                     WVKGRNYPFDFAVRMLDQDNKAHEYFFGNLFFDNWRKLTWVNPNYIDQVKDRVIIRKP
                     MYPKDLPYLKFSSFVVYRNMDQIGGDFILYIKNVSMRYEWHAAKVIENDIDDEEVWKI
                     LQQRAIDNMQREQKGLAEKAHDLKQNLKLQQEGNVQ"
     gene            5606..7321
                     /locus_tag="PLD82_00735"
     CDS             5606..7321
                     /locus_tag="PLD82_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG63945.1"
                     /translation="MPIKRSLRLHLLAGLVLVVMTASVIIQYLVSRDTLRTKDNDVMR
                     IFIEREIGNIDGWIREHGQMINLAGSLVQKAGETESEILHYLTTFLESGRDFVTLYFG
                     TPDNRMINASKWRMPPGFDLRTRPWYLEALRAGDLTTTPVFLNASRDRLIITVCKPVY
                     DTAGILKGVVAGDLPIDSLLGRIATTSPDTAGCSFVVGTEGRLLAHSSYLSNRNPLEV
                     PFPDPDYSPVLARITHGKTGTFETTLAGVSGFLVFKHMEQLDLIVAAFVPDDKLDQTL
                     GTLKITFFAIIAVSLVIWVSFVWYQRRHIDLPLALLEQGVTAIDPDARPWYRLPSIPS
                     KDFTPIVHTINSILDRIELYYNEKNSAETALRKSLQEKESLLREVHHRVKNNMQIVVS
                     LLESQADFQREPHSIEALQQIAQRVRSMAIVHEELYRSENLSQIPFAPVAEKIISSLA
                     DLLDHNHRIHWTIEDCDRGLDLVKAIPCGLILNELVSNVLKHAFAGNEPGVLRVSLTA
                     LHDAPPKKQFLMTVEDNGRGLPEGLDMSATRSFGLQLVKILCKQLQADLTLDRSNGTR
                     YSIFF"
     gene            complement(7324..8817)
                     /locus_tag="PLD82_00740"
     CDS             complement(7324..8817)
                     /locus_tag="PLD82_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63946.1"
                     /translation="MIERQNLRIEELERINEMHVRAEELSRQERIDAERMIEARERLQ
                     GLSEQERIAAEMTIRAQEGVQRLAEQELRQADKTIKAQETLHEFTEKERLAAEETIRA
                     QEEIQHLASQERLHAEQMLTAHDRIEMLTVSEIKQRDAVLAAILSMNVCMNALQPVDD
                     LLEHTLREACRLFSASRGFIARYTGASMTPIARSGFVYEAEADSILEAILAGRSVVGL
                     HAKLSREGRTIGVLYLERTMDADGFSAIDHEFLEILASQLSVGFNNSMLYHRLRQQNR
                     ELRRMIMLKNNFIEHLSSDLQRPLGKLVDLINGEDCSRQQEAINLAEWLRRTVNKVIS
                     MAALQNEVDEMYLHSIRMDIMIREIVENLGEEIRKRKLSIQYEFPEEVLPFEGNQDII
                     HTIMDEVICNAVVYNRDSGSVRIVINQDSQFCHITIMDTGIGIRKEELDKVFERFYRA
                     GTSHDMYNRGAGLGLYIVRNFIETYGGSISLSSIHGKGTEVALAFPM"
     gene            complement(8890..10584)
                     /locus_tag="PLD82_00745"
     CDS             complement(8890..10584)
                     /locus_tag="PLD82_00745"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOG63947.1"
                     /translation="MIRRISGVVVVLACIFAGTLGALSVAEQEIRRGAPVEFINYRGR
                     YTKIVTPEELRGVGRQLSSLATLSNRKYTAKRQYSVIRAIDLSVTNGFDADIFMLEPT
                     SDIVDINGVRDVLGGYLSGQYGYSDRDGRVLATFVTYYNAIYRGQIEYFRTKYKPVVT
                     SRLVQQFVGIDRNYRNWPGRTQMLVPLITSTNGGKPRIDIDGFTGREVITKLTNRPDK
                     GIPERRDLLEIKKDDLDKKQQDLDKDKKKLEDEKKKLEDDRSDLKKKEDEVTKQKEDL
                     KKETDPDKAEIKKDEIGKKEQEIEKKKDDIKKDEEKLKKDETTIGKKQDDLDKKKDDL
                     KKEETSIKKDETKLAEDKIPQVIKDDLKKKQDEVEKTKQDLEKKADEIKKREDELKKG
                     ELDRNVYDGGLYYLKVIEPLDNGHYKNEMVLINPVTRKVSLVSPFRNICGKRYDIFSG
                     GAVVVGYEGNHSSGHFLVVLDPKKLELVAKGKVNVYWQSFVEVKADSVYAVVQDAGYY
                     LGRFDAKLEQAARSKVQIDKDSFISFFGEYIYVNSPDKKIYVFKATDLSLVDTIQP"
     gene            10830..12098
                     /gene="fabF"
                     /locus_tag="PLD82_00750"
     CDS             10830..12098
                     /gene="fabF"
                     /locus_tag="PLD82_00750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG63948.1"
                     /translation="MKRDPSKTRVVVTGMGTINPIAHTVREYWENLAAGKNGIRLLQH
                     TDTTDYPVKIGGEVDLPENIADYMPKKMVNRLERFIVFAQIAAVQAFDDAGIRQEDID
                     REPYRYGAIIGTGDAGNWGHYKACRTIDKNNSVDMVSPFYVVGVIPNTPPAYFAKDRN
                     FQGPNFSVNSACATCNHAIGVAATIIKSGLADVMMAGGCESVVNTAGIAGFNAIGALS
                     RRNDDPATASRPFDKDREGFVLSEGAGVLCLEELEHAKARGATIYAELSGYGLSCDAY
                     DLVAPHPEGRGAAMAMSMAIESANLNLSDVDLINAHGTSTPLGDLAECRTIRRLFGDL
                     ADTIPVHSTKSMIGHLVGAAGGVEAIATILALYKGVIHGSMNVFEQDPELKLNVVANQ
                     PIERKIKHALSNSFGFGGQNSTIILSRFDG"
     gene            12171..12404
                     /locus_tag="PLD82_00755"
                     /pseudo
     CDS             12171..12404
                     /locus_tag="PLD82_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         2859 a         3489 c         2967 g         3201 t
ORIGIN      
        1 acagatagtc cggcatttgc tcctgtgtct cgaacaattc cttgagcttg gcatcaactt
       61 cttcctgctt cctgcgggca agaactcgcg agactctggc ttccatggct gcaaccagcc
      121 cgcggccaaa ctcctcgaaa aaatttgtcc ctcgggggta gatatgcgcc tcatactcca
      181 ggttttttgt gatatatctg atcaggagac acaactgctg gccgttcaca attttcgaca
      241 gagacgctgg aatcatgcgg tacccgcctt cggccatttt ctgaaaatga accagcgtct
      301 tggccacggt ctcttcatca gcagtttttg atccgttttc ctggaaatac gctgccgcca
      361 gcccaaggac agatggcaag gcttttgtat tgacagccag caggatactt tcgagatcct
      421 tgagcatctc gccagtccca tccgggcgga tgtcatgaaa gtcgggattt ggatcatcca
      481 ccgagtaccc cgactgaaat cgttttagga aagaaaagaa attgtaggag agcaattcct
      541 ttacgttgat aaaatctgca aatgttcgat tcagtctccg tcttgtctct tcgtcgatct
      601 tccgtatgaa gatatccgtc atttcatcaa tccgtttccg gaccatcgaa tcacggtcgc
      661 ccttcacgta ctccttgata ccatcttccg taaaccggaa tatttcttca tctgtaatgg
      721 ttggtaccaa ggtccggaca taaaagagct gaaaatcaag atgactctcc gggtctgttt
      781 cgccaaagac ctcttcaaag acaatccgat acggccagac cagcttgaaa aaatcatgta
      841 tcaaacccgg aaactcggcg gtgatcgctt cagtctggaa attgtagact gccggcttga
      901 gtttcttgat tgccagcttg agctctttga tgcgtttttt cttttcataa tctgcgggcg
      961 agagtccggt aaacaacatc acaaaaaatc ggaccaggcg ttcaaagaat ccggccttct
     1021 tcacgtcttg ctggccttgt ttgcctcttt tttttccgga tttgttgtcg gatgcctcgg
     1081 ccttgttcat gaggtcacgc tgagcacctt caaaaccctt ggccatgcgt tttttctcgt
     1141 cttcactcag cccgagcgca agaggatcaa agctgtcgtt gaatatgcct ttatccttgg
     1201 ctttgcgaat cgattcctca tcgtaggaca ttttctcacc ctttgtcaaa agaggtttcg
     1261 tgacagcgcg gccatcagat ccatgatgaa tgcgatggca aagtgcagca ccggacatac
     1321 ccagaatgat cgtgcccgca aaacgagaat accgaggacc cacgcagcaa taatggaacc
     1381 aaatgcctcg gcaacaggct ttccggcatg catgatcgca aacgggacaa gaccgaccag
     1441 tgcggccagc gatccgatcc tgcgggaaag cggaaaaatg agcaatcctc gaaagaaaaa
     1501 ttcccaaccg acaaagtaga gaaaaaaaca agtttcaaga atgatgaacc agaaaacaaa
     1561 atcaggctga tgaccggtta tccccttgac ggcaaacatc gggtaatacc gtaaaaaacc
     1621 ctcgatatgg aaaaccttga tggctgcaac agccagagcc atcacgacgg caaacgctga
     1681 aaaaattttc aatgagaata gccagtcccc aagaccaaga ccatagtcct tcagtcgcag
     1741 ccgtggatgg acagctataa aaacaaacgg tagcacaaaa agaatgaaga atgtcgacaa
     1801 aaaccatccc gcatatctga gataatgaat gctccgtccg cgttctcctg caaccgcact
     1861 gtccttggag accatggcat cgagtttctg gattttttgt actgtctccg ccgttcccgg
     1921 ttgctttttc gtttccagtt tgagccggtc cagttcgatc cgtctctccc cggcggccac
     1981 atctcccgaa atcagggccg gcaagccgta cccgcttccc ttcatgaagg actcttttcc
     2041 aagatagatg tagatcatga aaagtgcggc tatggccatg aatcccagat gaacccaccg
     2101 gttccttccg gtgagctctc tggtgaaagg tgaaaacaga ttacgtaaat ttccaagcat
     2161 ccatcagttc ctgtttcgga taaaagcctg ctcagccttg ctgcggactt tttcaagaac
     2221 ctggtcggca tctgcttttg cggcaaatgg gcccacccag acacgatgca gcctttcgcc
     2281 ggaaaactgc cggatgaaga caggaagccc catggcctgc aggctcctta aaagactctc
     2341 ggcattgtca aggctcaaaa aagcgccggc ctggatgaca atcttcccag tctcaacggg
     2401 tttgactgtt gcgttgtcat catttttatg ctgaacatcc tcgggccgga taatcctcgg
     2461 cccatccgtc agagtcgtcc cggttcccct gggtgtttcg acagcccctc cattggtccc
     2521 ttgatccgca ggatacaccg gattctggtt ggcattcgag gccacggtca cagcgctgtt
     2581 tcgtgtgtct tcatttctgg tgagcgccgg aaccgcgctg gccgctgttg tcgcagcctg
     2641 catctttccg tctccaagct gaaccaccct gatttccact ttcgcagtcc ctttcgtccg
     2701 gaaatcaagc agggatgccg cagcccgtga tacatcgatg acccgttcct tggcaaacgg
     2761 gccccggtca ttgatgcgga cagtgacgct cttgtcgttc tcgagattcg tcacccggac
     2821 aagtgttcca aaaggaagcg tccggtgggc tgctgtcagc atcgtcatgt cataggtctc
     2881 accactggct gtcgttcttc catggtacac atcaccgtac caggaagcct caccataaaa
     2941 cagcatcggg ataccggctg tatcggctgc cgaaagcggc aatccggtta cgacaagcac
     3001 aagtacaaga gcgagcagtt ttttcatttc gatacgctcc acattctaca ggaatttgtt
     3061 tcatttatcg gtacaatacc aaaatgactg tagactgaaa ctcaggagcg agggaatgga
     3121 caagcagagc ctgtatcgat tcggaaactt cttcccgaaa gttgatccct cggtgtatat
     3181 cgctcccggt gccaggatcg tcggcgatgt caccctcggc gagaacacca gcatttggtg
     3241 gaacaccgtt ctccgtgccg atctcgcacc aatcatcgtc gggccacgtt ccaatatcca
     3301 ggacaacgcc acactgcatg tcggtcgcaa ccaggcgtgc attctcggaa cggatgttct
     3361 ggtcggacat ggcgcaatct gtcatgcctg taccatcgat gatggtgccc tgatcgggat
     3421 gggagcgatc atcctctcgg gtgtcaccat cgggcatcat gccctgatcg cggctggttc
     3481 ccttgtttcc gagcggacag agattccccc gcaaacactc tgggccggca atccggcccg
     3541 ttgtatcaag agtctcggcc ccgggatgct cgaacgcatg cgggaaggtt cggcactcta
     3601 tctgagcctg gctgctctct cgagagacga cggccctcac cgtgatactg actgatcaaa
     3661 aacctgtcgg cgcatcaaga aagcaggtct cgactggcca ggaagtggcg caatgcttca
     3721 tcagtcgttt gatgccaacc gtttcgctca gataatggcc gtacaacagg cagacgattc
     3781 cgcattcgac aatctggtga taggaagcat ggctcccctc cccgctgatg aacagctctg
     3841 cgccgagtcg tgccgcctct tccgcatatt tcagtccgct tccggtgatg gctcctgcgg
     3901 aatggaacac ggtatccgga gaacactcat tcaatatccg tgtcggtccc gaaccaagcg
     3961 tcttctccaa tgcccctatg acgctctcga aacttctgcc ctcctgagac tgcccgaagc
     4021 ttccgacctt ggtaccacgt tcttcagcaa aatttcccgt gatggtgaaa cccgcatcct
     4081 caaggattcc cgcattgttt ccatacagag gatggacatc cagcgggaga tgggcggcgt
     4141 acagcgagag gttactctcg atgcaggcag ccacgcgctg tcggtgcgta ccggtaatca
     4201 tcaagggctg attccaaaag agaccatggt gcaccacaag caaatccgct ttggcctcga
     4261 cggcaagctg gatcgtcttt agcccggcat ccacggcaaa agcgattctc cggatctggt
     4321 cacagccttc aatctgcaaa ccattattcg accagtctgc aatcctcttc cgttccagga
     4381 gggcatcaag ccctcctgtc acctcgtcca gcaacatcac tggacattcc cttcctgttg
     4441 gagcttgaga ttctgtttga gatcatgtgc cttctcggcc aatcccttct gctcacgctg
     4501 catgttgtca atcgcacgct gctggagaat cttccagact tcctcgtcat caatatcgtt
     4561 ctcgatgact ttggcggcat gccattcgta gcgcattgaa acattcttga tatagaggat
     4621 gaaatcgccg ccaatctggt ccatgtttct gtagacaacg aaggaactga acttgaggta
     4681 ggggagatct ttgggataca tgggttttct gatgatgaca cgatccttga cctgatcgat
     4741 gtagttcgga ttgacccatg tcagcttgcg ccagttgtca aaaaagagat tcccgaagaa
     4801 atactcatga gccttgttgt cctggtcgag catccggacg gcaaaatcaa acggatagtt
     4861 tcttcctttg acccagaccg agacatcttt gaccagcccg acattggtca caatcccgtt
     4921 cgagatgttc gagaaatttc cttttttgtc atagacgtgg aactcgaact gtggccggat
     4981 ataggccctg tcgttctgcc gggtttcggg gaaattgatc ctgacaccaa ggacattctg
     5041 gttctgcaat ttgctgaaaa ccgaaagaca ttgcgatttg acaatatttg gaatccggac
     5101 accggaagaa cggacttcga caatccagtt actgatggcc atcaccgatg gcgtcaggat
     5161 ataattcggt gatccatccg ggtttttcgg tttagagttt gtactgatac tctggaattc
     5221 ggctgtaatt cttttttcgt aattgccaaa attgatcaca gtgatgtcgc tcaatgcatg
     5281 aacgatcatt ggaaccaata aagccaatga cagaattaca aatacaacaa ttttcatccg
     5341 cttttgtaac aacatgtcga ccctccggca tacgacacct ctgtctctcc cgaatactcc
     5401 gggacgggca tcgattgtgc acatcatggt ttttgaaatc catttctgat ggtgactgcc
     5461 gggaagtccg tttccctctc agatgatcgg attcccgtca aaaaggttta gtggtttcgc
     5521 accaatcccc ggaaacaagt cctttcaaaa caccatcatt gtcagtacaa tagtgggaga
     5581 atctaccttt atggaggtgc cggacatgcc aataaagcgc agcctgagat tgcatcttct
     5641 ggcaggtctc gtgcttgttg taatgaccgc atccgttatc atacaatatc tagtctcccg
     5701 ggatacgcta cgcactaaag acaatgacgt catgagaatc tttattgaac gagaaatcgg
     5761 caatattgac ggctggatcc gtgagcacgg acagatgatc aatcttgcag gatcactggt
     5821 gcagaaagcc ggtgaaaccg aatccgagat actccactat ctcacgacat ttctcgagtc
     5881 cggtcgtgat tttgtaacac tgtattttgg gacgcctgac aaccgcatga tcaatgccag
     5941 caaatggcgg atgcctccgg gatttgacct tcgcacacgc ccttggtatc tcgaagcact
     6001 tcgtgcaggc gatctgacaa caacacctgt ttttctcaat gcaagcaggg acaggctgat
     6061 cattactgtc tgcaaacctg tctatgatac agccggaatt cttaaaggag tcgttgccgg
     6121 cgatcttccc atcgattccc ttctcggtag aattgccact acttctcctg ataccgccgg
     6181 atgttcgttt gtcgtcggca ccgaagggcg attgttggca cattcttcat atctgtcaaa
     6241 ccggaacccg cttgaagtac cgtttcctga tcctgactat tcccctgttc ttgccagaat
     6301 cacgcatgga aagaccggta cattcgagac cacccttgcc ggagtatccg gttttcttgt
     6361 cttcaagcac atggagcaac tggatctgat tgtcgctgcc tttgttccgg acgacaaact
     6421 ggatcagacg cttggaacac tgaaaatcac atttttcgcc atcattgccg tctctctagt
     6481 gatttgggtt tctttcgtct ggtaccagcg tcgacacatt gatcttccgc tcgctctcct
     6541 cgagcaaggt gtcacagcga tcgatcctga tgccaggcct tggtaccggc ttccatcgat
     6601 tccttcaaaa gacttcaccc cgatcgtcca taccatcaac agcatcctgg acaggattga
     6661 attgtactat aacgagaaaa actccgccga aaccgctctc aggaaatctc ttcaggaaaa
     6721 agaatctctt ctcagggaag tccatcaccg ggtgaaaaac aatatgcaaa tcgtggtaag
     6781 cctgcttgaa tcacaggcag attttcagcg cgagccccac tccatcgagg cattgcaaca
     6841 gatcgctcag cgggtgcgga gtatggccat tgtgcatgag gaactgtaca ggtctgaaaa
     6901 cctttcccaa attccttttg cgccggtcgc tgaaaaaatc atctcgtcgc ttgcggatct
     6961 cctcgaccac aatcaccgca ttcactggac tattgaagac tgcgaccgtg gtcttgatct
     7021 cgtcaaagcc atcccctgcg ggttgatcct caatgaactt gtctccaatg tcttgaagca
     7081 tgcttttgcc ggaaatgagc cgggcgttct gcgtgtttcc ctgaccgctc tccatgatgc
     7141 cccgccaaaa aaacagtttc tgatgactgt tgaagacaat ggtcgcggat tgcccgaggg
     7201 gctggacatg tcggcaaccc gttcgttcgg actgcaactg gtcaaaatcc tctgcaagca
     7261 gctccaggca gacctgacac tggaccgctc caacgggacg cgctattcca tctttttctg
     7321 atgttacatc gggaaggcaa gggcgacctc tgtccccttg ccatggatgc ttgaaagaga
     7381 aattgaacca ccgtacgtct cgatgaaatt ccggactatg tacagcccga gaccggcacc
     7441 cctgttgtac atgtcgtgcg aggtcccggc gcggtagaat ctttcaaaaa ctttgtcaag
     7501 ctcttcctta cgtataccaa taccggtatc catgatcgta atatggcaaa actgggagtc
     7561 ctggttgatg acaatcctga cacttcccga gtcacggtta tagacaaccg cattgcagat
     7621 tacttcatcc ataatggtat gaatgatatc ctggttcccc tcgaagggaa ggacttcttc
     7681 cgggaactcg tactgtattg acaattttcg ctttctgatt tcttcgccga gattctcgac
     7741 aatctcccgg atcatgatat ccatccggat actgtgcaga tacatctcgt caacttcgtt
     7801 ttgcagggct gccatcgaaa tgactttgtt gaccgtccgc ctgagccatt ccgcaagatt
     7861 gattgcctcc tgctgccttg agcagtcttc cccattgatg agatcgacca gtttcccgag
     7921 aggccgttgg agatcggacg agagatgttc aataaaattg tttttcagca tgatcatccg
     7981 gcgaagttcg cgattctgct gacgaagtct gtgatacagc atcgagttgt tgaacccgac
     8041 cgaaagctgg gatgccagaa tctccaaaaa ttcgtgatcg atggccgaaa agccgtccgc
     8101 atccattgtc ctctcgagat acaggacccc tattgtgcgc ccctcacggg acaacttggc
     8161 atgcagtccg acgacagagc gcccggcaag aatggcctcc agaatgctgt ccgcctcggc
     8221 ttcgtacaca aaaccgcttc gggcaatggg agtcatgctt gcacccgtat accgtgcaat
     8281 aaatcctctg cttgccgaaa aaagccggca ggcttcgcgg agtgtatgtt caagaagatc
     8341 gtccacaggt tggagagcat tcatgcagac attcatcgac agtattgcag ccaggacggc
     8401 atcccgctgt ttgatctcgg aaacagtcag catctcgatc cggtcatgcg cagtcagcat
     8461 ctgctcagcg tggagacgtt cctgggacgc caggtgctga atctcctcct gggcccgaat
     8521 cgtctcctcg gcggccaacc gctctttctc cgtgaactcg tgcaacgttt cctgggcctt
     8581 gatggtttta tcggcttgtc tgagctcctg ctcggcgaga cgctgcacac cttcctgcgc
     8641 tcgaatggtc atttctgccg cgatacgctc ctgctccgaa agaccttgga gacgctcccg
     8701 ggcctcgatc atcctttcgg catcgatgcg ttcctgcctc gacaactcct cggcccgaac
     8761 gtgcatctcg ttgatgcgct caagttcctc aatcctgaga ttctggcgtt cgatcatgag
     8821 cctcagtgat tccaacaaat caccttggtg ttcatcccgc tttcgtttct gtcccatttc
     8881 tcttccggat cagggctgaa tggtatcgac caggctcagg tcggttgctt tgaagacgta
     8941 aatcttttta tccggcgagt tgacgtagat gtattcacca aagaagctga tgaagctgtc
     9001 cttgtcgatc tggaccttgg aacgagcggc ctgctcgagt tttgcgtcaa acctcccgag
     9061 atagtacccg gcatcctgga caacagcata gaccgagtct gccttgacct cgacaaaact
     9121 ctgccaatag acgttgacct tcccctttgc aaccaactcc agctttttgg ggtcgagcac
     9181 aacgagaaaa tgcccgcttg agtggtttcc ttcgtagccg accacgacag ctccaccact
     9241 gaaaatgtca tagcgcttac cgcagatatt cctgaacggg gaaacaagtg aaacctttcg
     9301 tgttacggga ttgataagca ccatttcatt cttgtaatgg ccgttgtcga gcggttcaat
     9361 gactttcaga tagtacaaac cgccgtcgta cacattccgg tcaagctcgc cttttttcaa
     9421 ttcgtcttca cgctttttga tctcgtccgc ttttttttcg agatcctgct tggttttttc
     9481 gacctcgtcc tgcttttttt tcagatcatc cttgatgacc tgcgggattt tatcctccgc
     9541 cagtttggtc tcgtccttct ttatcgaggt ttcctctttt ttgagatcgt ccttcttttt
     9601 atcgagatca tcctgctttt tcccgatcgt cgtttcatct ttctttaact tctcttcatc
     9661 tttcttgatg tcgtcttttt tcttttctat ttcttgttcc ttttttccga tctcgtcttt
     9721 cttgatctcg gctttgtcag gatccgtttc ctttttgagg tcctctttct gtttcgttac
     9781 ttcgtcttcc ttttttttca gatccgatct gtcatcttcg agcttcttct tttcatcctc
     9841 gagcttcttt ttgtctttgt cgaggtcctg ctgtttcttg tccagatcat cttttttgat
     9901 ctcgagcaaa tccctccgct cagggatccc tttgtcagga cggtttgtca gtttggtaat
     9961 cacctctctc ccggtgaacc cgtcaatatc aatccggggc tttccgccat tggtcgaagt
    10021 gatcaaggga acgagcatct gagtcctgcc aggccagttc ctgtagtttc tgtcaatacc
    10081 gacaaattgc tgtaccaacc tggaagtgac aaccggcttg tacttggtcc ggaaatactc
    10141 gatctgtccg cgatagatgg cattgtagta cgtgacaaaa gtcgccagga cccgcccgtc
    10201 ccgatcggaa tacccatact ggccggagag atacccgccc aggacatcgc gcactccgtt
    10261 gatgtccaca atgtcgcttg tcggttcgag catgaagata tcggcgtcaa aaccgtttgt
    10321 gacagagaga tcgattgccc ggatcaccga atactgtctt ttggcggtat acttccggtt
    10381 ggagagggtt gccagcgacg agagctgacg gccaacaccc cgaagttctt cgggggttac
    10441 gatctttgtg tagcggcccc gataattgat gaattcgacg ggcgcacctc gccggatttc
    10501 ctgttcagct acagacaaag cacccaatgt cccggcaaaa atgcatgcta aaacgacaac
    10561 aacacccgaa atccgccgta tcatactctc ccccattctt gatcctctct gtctccagtt
    10621 tctattcata gcacatcttg aaccgtatgt caaaatttgc caaataccgg taaggacgga
    10681 cttgacccga gctttattga gtggttattg tcgtaggtac gggtctgttc tgttttgggg
    10741 gtcttgtttc actccgcaga ctgttctttt tcccgttttc gtatccgtgc cgcatagcac
    10801 aaccatccaa ttctggagat gaactgctca tgaaacgtga cccttccaaa actcgagtcg
    10861 ttgtaacggg aatggggacc atcaacccca tcgcgcacac cgtccgggag tactgggaaa
    10921 atctcgcagc cgggaaaaac ggtatccgtc ttcttcaaca caccgatacc acggattacc
    10981 cggtgaagat cggtggcgag gttgatttgc cagaaaatat cgcagactat atgcccaaaa
    11041 agatggtcaa ccggctcgaa cgattcattg tttttgccca gatcgctgcg gttcaggcgt
    11101 ttgatgatgc ggggattcgc caggaagaca tcgatcgcga accgtatcgc tacggtgcca
    11161 tcatcggtac gggagacgcc ggaaactggg gccactacaa ggcctgcagg accatcgaca
    11221 agaacaactc cgtcgatatg gtttcccctt tctacgtcgt cggagtcatt cccaataccc
    11281 cgcctgcgta ttttgccaag gaccggaatt tccagggacc aaatttttcc gtcaattctg
    11341 cctgcgcaac atgcaaccat gcgatcggtg tggctgctac aatcatcaag tccggcctgg
    11401 ctgatgtgat gatggccggc ggatgcgaga gtgtcgtcaa tacggccggg attgccggct
    11461 tcaacgccat cggtgccctc tcgagaagaa atgatgatcc tgccaccgcc agccgtccct
    11521 ttgataaaga ccgcgaaggc tttgtcctga gtgagggcgc cggggtgctc tgtctggaag
    11581 aactcgaaca cgccaaggcg cgtggagcaa ccatctatgc cgaactctcg ggctacggcc
    11641 tttcctgtga cgcctatgat ctcgttgcac cccatcccga aggacgtgga gccgccatgg
    11701 ccatgtcgat ggccatcgaa tccgccaacc tcaatctctc cgatgtcgat ttgatcaacg
    11761 ctcacggaac ctcgaccccc ctgggagatc tggccgaatg ccgcaccatc cgtcgtctct
    11821 tcggcgacct ggccgatacc attcccgttc acagtaccaa gtcgatgatc ggacaccttg
    11881 tcggagccgc gggtggcgtt gaagccattg cgaccatcct ggccctctac aaaggggtca
    11941 tccatggttc catgaatgtc tttgagcaag atcccgaact caagctgaat gtcgttgcca
    12001 atcagccgat cgaacgcaag atcaagcatg ccctttccaa cagcttcgga ttcggcggac
    12061 agaattcaac catcattctt tcgcgctttg atggctgatc acgtcagacc ggattgtatc
    12121 ccgcagaaac gcccccggag ggggtgtttt tgcatgcaca ggcctgccgc atgacgctgc
    12181 aatcctgccg gctctgcggc gcctgctgca tcgcgccatc aatctccagt ttggacaaac
    12241 ccgcgggaat acgctgtcag catctcacaa gcacgaacac atgcagcatc tttggatcag
    12301 ccggccggcc ggcggtctgc tcgtcgtatg ccccgggtac cgactgcggg cattcgtttt
    12361 tggaggccat ggagaaactg acacggttgg agacactgac ttcgcctgtc cggactgacg
    12421 acccgctgaa ttaccccgga gggcaggctt cctggcccac ccttacaggg aatccaggga
    12481 aagcacgagc cgaacgcctg tctcaacctg cttgag
//
LOCUS       DAOSHE010000011        12363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4298_length_12363_cov_4.646653, whole genome shotgun sequence.
ACCESSION   DAOSHE010000011 DAOSHE010000000
VERSION     DAOSHE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_12363_cov_4.646653"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /gene="ahcY"
                     /locus_tag="PLD82_00760"
     CDS             <1..1271
                     /gene="ahcY"
                     /locus_tag="PLD82_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG63949.1"
                     /translation="IQTAVLIETLVELGANVRWSSCNIFSTQDQAASAVVVGKKGTVS
                     APAGVPVFAWKGETLLEYWRLLARTLDWGGGKGPELIVDDGGDATLMMHLGCDVEKDP
                     SLLDKKVETEDEIALFAVLKEIYAKDKTFWQKHVADWRGVSEETTTGVHRLYEMEKSG
                     TLRVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVCGYGDVGKGSA
                     QSLAGLGARVIVTEIDPICALQAAMAGYEVTTVEDTLGRADIYVTTTGNADVITAEHM
                     MKMKDQAIVCNIGHFDNEIQVAALKKMPGVTITNIKPQVDKYTMPDGRAIFLLAEGRL
                     VNLGCATGHPSFVMSNSFTNQTLAQIDLWKNRDTITKGVRTLSKLLDEEVARLHLEKI
                     GVKLTKLTPKQAAYIGVPVEGPFKPENYRY"
     gene            1410..5597
                     /locus_tag="PLD82_00765"
     CDS             1410..5597
                     /locus_tag="PLD82_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG63950.1"
                     /translation="MEVRIAIAEDDEMTVLELESNLKRLGYGVSGHARSGPALLDLVR
                     EKHPSVVLMDINLEGAMDGIETAAIVDREFKIPVVFLTASGDPDTLGRAVGSNPFGYL
                     VKPVDPVLLRIGLEMALYKHKTDSWMRENEHRLYAILDSMGDGVIAVDTDEKVIFINQ
                     VAAELFGMEFSQVCGKQLDEIFQIIRTGEGEGGLGKAFLLRSLGESLPIEWRRTDLAG
                     SDGGAVIVFRDISGQRRVEERLALQNAAMEAAANAIVVTDVNGDILWVNKAFCRLTGY
                     GRDEVIGKNTRILKSGRQDPQVYEDLWRTILSGRVWRSELVNRSKDGGFYTVGITITP
                     IRDSDGGISRFICIKEDISERKKAEEALRRSEEYYRALIENGTDIIFIVDPGGVVTYV
                     TPSCRRVFGLGPEDLTGRQIADLVLESGRARIREKLREALLLRGEVRLIEMHVEHSDG
                     KESRILEGLFRGVIDAEGRERVIISARDITERERNVQEIVTLARISAENPNPVLRISS
                     DGVIIYGNRASIQVLHEIGAQLGQTIPDEFRAIIRDVILAGRNREVRMSFGDHIYSML
                     FVPIVPEGYVNLYGTDITERTRALSALQKSERQYRAIVEDQTDLICRMNPDYKLLFVN
                     DAFCRFFNLSHGETVGLLFAGILGEKEARRFAAGIADLGVDNPVLVIEQEISGDGGTR
                     WLEWTCRVLFDLSESIIEIQGVGREITDRKMLAEMAREKEAAEISNRMKSEFMSNMSH
                     EIRTPMNGILGMTELMLDTALTGEQRDYALTIHRSSQALLTIINDILDFSRIEEGKLK
                     IEEAEFDLGQAVEDIASLLAPRASEKGLELLVHFSRLAPRLVIGDQGRVRQIILNLVN
                     NAIKFTHEGYVLIHVDHVARRDGSNAFTIAVEDSGIGISADKLDVIFERFSQADTSTT
                     RFYGGTGLGLTISRQLAQLMHGDIVVNSSPGTGSTFWFTVPLPLSPNPQPVFPDLAAF
                     QENVSCYLVDAYDVTMRVHGEILADLGLPHIVSFGHEVCDFLKRDDPGRIRIVMIDER
                     VEDVDCFVVARKVRELFSDDKVKLVLLANPETILEDLHDKGVVFDRVLYKPLRPRLFA
                     EQMNALISGEKSPASSVHVPGNVRHDIRESDLTEPEARSTEVVLDASILLVEDNLVNQ
                     RVAQRMLQKLGCRVTIAGNGRQALESLSGGHFDLVLMDCQMPVMDGYTATRELRNPAS
                     PWYAPDLPVLALTAHAGESERERTFAAGMNDHISKPLRSSVLLEKLTTWLKTGGVPEG
                     EPGTGSDEPVQPEEEKPAPVFDRAGAYERLGDDDGLLRELIQVFFGDLDKQLSALRGF
                     LAEGNAPAAENTSHALKGGSSNIGAERIRRRFYEMEIAGREGRLEDLRAALESIPGLI
                     AELENELKAQGYPVDPS"
     gene            complement(5610..6674)
                     /gene="hflX"
                     /locus_tag="PLD82_00770"
     CDS             complement(5610..6674)
                     /gene="hflX"
                     /locus_tag="PLD82_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG63951.1"
                     /translation="MHTHQHEAPAVITISQGLPAESEEIHLLAESCGYQVLADLSLST
                     RSRPGHGLPGSGKLAELEEIRNTSGCDFFILSYDLAPSRKRSFENAVGCPVMTRTELI
                     LEIFARRARTAIARQQVELARLEYLLPRLEGGYAHLAASQGGSGFRGPGETQIELDRR
                     RIKNRISLLRRRLAAVKTSRREQRKKREKNIPLVALCGYTNAGKTTLMNRLAKTNLQT
                     EDRLFATLDPTVRQLHNPNGSPMLLVDTVGFIRDIPHTLVEAFASTLEEITRADLVLV
                     VFDASDHAADQEIPVVESVLAEIDADRIPRLYCANKIDMETGLPSRELAGKPLVRVSA
                     KTGEGIDNLLRMLHTTLFKS"
     gene            6798..8558
                     /locus_tag="PLD82_00775"
     CDS             6798..8558
                     /locus_tag="PLD82_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63952.1"
                     /translation="MAEIPDKSRQQQVILDDDDEIISLSDAELDTILGSAEITDAPIA
                     DIEIDIISGPASRPAPAEAGRGQTGDDTPQPEAGGGFFSSDDDETISLPEDELETILD
                     TGTYEADGMEPSLPESEVVVAGSEQVIPDMLETPSLGGEPDVEPDFGGPETPIDEGVD
                     DAFIIIDDQPLESSGLGMDSGPMIIEEEISPDTAMPELDFAREQAARSNDEPVVEVTG
                     DSMDDLFDDDAEVPVDGAGEDVLDDFEVEDHGAEINLSPPQPVPVADIPGIDALADEP
                     SPLEIQPAPPEFFLEEGAAEYDSESVDDLLIEEAPILDQQPVERNELRDTIIDVEDEL
                     IDLEPIERREDLEDSSRSEVVFDDEAEGDTVVSLPVGDGAAEAGYEAGLEEETVLETG
                     SFVAEDGIPPPELPGEEPVIGEPPRAEVAKDDGFVRAPDFEEEIASLSLEEAPAGTVP
                     VVDQVDLADEPIEFMEDEIVFAPHQTIPAAEPVSDIAPDDDLLDEEIINPDMISEMKD
                     EVFDEPDSPDDEPVASDEVRPPSQEVVEGMPERMRDDVRKVLGYLDNLFDELPEDKVK
                     EFANSEYYDIYSRLFKELGI"
     gene            8562..10127
                     /locus_tag="PLD82_00780"
     CDS             8562..10127
                     /locus_tag="PLD82_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63953.1"
                     /translation="MGLLSRARHVGFRRTPAQDSPASGRRGLLTRAGMVRRARAQSPG
                     PVDDPSPETGSLRQLERQSFNLSNLMEISREINGTLRLDDLCTIIILTVMGQSGAQKA
                     VLYVRDDEEYVVRAARGLAEGLVLPSLPVDPVFAQTAVQRDGVFPVSACAFVDGSLET
                     RLVSLLSGKLVVPFVNKGQCLAFLLLGERYPDQDWNEDEIGFLASIADMGSMALANAR
                     LFGELEKKLGQLSGLYEISRVINGSSEVGEILRLAGETLGTGFGVNRAAFLGLAGHDL
                     VFLSGIGIEGIRAGSTMTPLFESVFTLEQALVIEDRSGNPELSAVFGEGVLEESNMAL
                     LVPLVAAGERVGILAVLGVEGRRSGTVLQEECELFSIIGSQFAPPLLVARTLGDRKRS
                     LADPFHPWMELLEREVARAGEFGLSVGVCELSLDGIDERDGDMVRDLGRVLRDRIDLR
                     YPVVRLAAASWAVVLSGLSLRDQERLIDSVTQEILTLLDRSGDAERIRLERSHGQIPE
                     EYPSAAAWVFRRL"
     gene            10141..11028
                     /locus_tag="PLD82_00785"
     CDS             10141..11028
                     /locus_tag="PLD82_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG63954.1"
                     /translation="MNLTEQRVHEGDISGSSFSFPGRVEITGSVRAGAVVHAGGDIVI
                     RGSVEAGLVESSGGGVYIDGEIHGPGAVVRAFGDVKARVIRDAEVESLASVYVPDMIF
                     RSAVRARNLIEMKDGDGIVEASYAEAGMEITIRSAGSVSSGMDPTTLVLENFRQKELF
                     ELSMIYEQRLTQKTARIAELEKVISVIRILGGRIVNLDPAKKADLALKVKEYQELKQQ
                     VVEILRERDLVVVEREKSGGKMRSITSSGDVFSGVEVRIDNARVVVNRRYKNVIFYKS
                     GIVIIGDLDAFMQRKRLSN"
     gene            11054..>12363
                     /locus_tag="PLD82_00790"
     CDS             11054..>12363
                     /locus_tag="PLD82_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63955.1"
                     /translation="MGIGFKLGMFVAVVLSLVMLVTGVFIVRQQETVLDNQLRRDLSS
                     HLKSFSMAVTASLSGKEDLPRLQSSTYALSNINNFGTAMFVDPRGRVFAQVDRSGVPF
                     DGRYAVVTMPIPKLDDPELAARYPDIGYLLSDMVDGTNIIFARSSRYTSEEYAQVLAE
                     SASNKTPPPVNTAWFEGFLPVCVNWLQQVHPGDNSLDKALALYRAGVTNGFLFRGVQN
                     DQRRRDLAFLRHIETVARWLAVEKLDPWGNRVGWELSPYAKMLGLREKDRLTETESWQ
                     IAAMIPLIEGFRKGESAGSPEKLFPRETITRITSLVERYFSGAAMPDGLGGLLTDARA
                     GKLLMAGVSPGEEAEIRMAADWLEVARALTRSSGPEWNKVYGEMAAARQIAPFVHGDE
                     IFVDIALFAGLFEAYLTGGEFPMPETHRYRKLSRIYEPKKSPEER"
BASE COUNT         2501 a         3339 c         3885 g         2638 t
ORIGIN      
        1 ccatccagac cgcggtcctg atcgagaccc tcgtcgagct gggtgccaat gtccgctggt
       61 caagctgcaa tattttttcg acccaggatc aagccgcttc ggccgtcgtg gtcgggaaaa
      121 agggcacggt cagtgcgccg gccggtgttc cggttttcgc ctggaagggc gagaccctgc
      181 tcgaatactg gcgcctcttg gcccggaccc tcgattgggg cggcggcaag ggacccgaac
      241 tgatcgtcga tgacggcggc gatgccaccc tgatgatgca cctgggctgc gacgtcgaga
      301 aggacccctc cctgctcgac aaaaaggtcg agaccgaaga cgagatcgcc ctctttgccg
      361 ttctcaagga gatctacgcc aaggacaaga ccttctggca gaagcacgtg gctgactggc
      421 gcggcgtttc cgaagaaacg accaccggtg tccaccgcct ctacgagatg gagaagagcg
      481 gaaccctgcg cgttccggcc atcaatgtca acgactcggt caccaagtcc aagttcgaca
      541 acctctatgg ttgccgcgag tccctcgtgg acggcatcaa gcgggcgacc gatgtgatga
      601 tcgccggcaa ggtggctgtg gtctgcgggt acggcgatgt cggcaagggt tctgctcagt
      661 ccctggccgg cctcggggcc cgcgtcatcg tgaccgagat cgacccgatc tgcgccctcc
      721 aggcggccat ggccggctac gaggtgacga cggtcgagga tactctcggc cgggccgaca
      781 tctacgtgac gaccacgggc aatgccgatg tgatcacggc cgagcacatg atgaagatga
      841 aggaccaggc catcgtctgc aacatcggac actttgacaa cgagatccag gttgcagccc
      901 tcaagaagat gcccggggtc acgatcacca atatcaagcc ccaggtggac aagtacacca
      961 tgcctgatgg ccgggccatc ttcctcctgg ccgaaggccg cctggtcaac ctgggttgcg
     1021 cgaccggcca cccctccttc gtgatgtcca actccttcac caaccagacg ctggcgcaga
     1081 tcgacctctg gaaaaacagg gacacgatca ccaagggtgt ccgcactctc tccaagctgc
     1141 ttgacgaaga agtggcccgc ctgcacctcg agaagatcgg ggtcaagctg acaaagctga
     1201 caccgaagca ggcggcttac atcggcgtgc cggtcgaggg ccccttcaaa ccggagaatt
     1261 accgctattg aggtccggtc attgctgttg ccaaaaaccg cccaggggaa acctggcgcg
     1321 gtttttttat tcttccggat tttttacggg gacttcgtgc gaaggggggg gattgcgttc
     1381 cgggctgaac ccgggatatg atggagagca tggaagtccg gatagcgatt gccgaagatg
     1441 acgaaatgac cgtcctggaa cttgagtcca acctcaagcg tctcgggtac ggtgtttccg
     1501 gtcatgcccg gagcggacct gcactgctgg atctggtccg ggaaaaacac ccttccgtcg
     1561 ttctcatgga tatcaatctc gaaggggcca tggacggcat cgaaacagcc gcgatagttg
     1621 atcgcgaatt caagattccc gttgtttttt tgacggcctc cggggatccg gataccctcg
     1681 gacgtgctgt cggcagcaat ccctttggct atcttgtcaa accggttgat cccgtcctcc
     1741 tgcggatcgg tctggagatg gcactctaca agcacaagac ggattcctgg atgcgggaga
     1801 acgagcaccg gctctatgcg atcctcgaca gcatggggga tggtgtcatc gcggttgata
     1861 ccgacgagaa ggtcatcttt atcaaccagg tggcagcgga gcttttcggg atggagttca
     1921 gccaggtctg tggaaagcaa ttggacgaaa tcttccagat catcaggacc ggcgaaggag
     1981 aaggcggact ggggaaagcc tttcttctgc ggagtctcgg tgaaagcctt ccgatcgagt
     2041 ggagacggac cgatcttgca gggagtgacg gtggcgcggt cattgttttc cgtgatatct
     2101 ccgggcagcg ccgggttgag gagcgcctgg ccctgcagaa tgccgccatg gaagcggcgg
     2161 ccaatgccat cgtggtgacc gatgtcaacg gggatatcct ctgggtcaac aaggcctttt
     2221 gccggttgac cgggtacggg cgggacgagg tcatcgggaa gaatacccgg atcctcaaat
     2281 ccggccggca agacccgcag gtctatgagg atctctggcg cacgattctc tcgggacgtg
     2341 tctggcggag cgaattggtc aaccggagca aggacggcgg gttctatacc gtcgggatta
     2401 ccatcacgcc catccgggac agcgatggcg ggatctcgcg cttcatctgc atcaaggaag
     2461 acatcagcga acggaaaaaa gccgaggaag ccctgcgcag gagcgaggag tactaccggg
     2521 cgctcattga aaacggaacc gacatcattt ttattgtcga tcccggggga gtggtgacct
     2581 atgttacgcc ttcctgcaga cgtgtgtttg gactcgggcc cgaggatctg accggcagac
     2641 agattgcgga tctcgttctg gagtccggac gtgcccggat tcgggagaaa ttgcgggaag
     2701 cccttctgtt gcgtggagaa gtgcgtctga tcgagatgca tgttgaacac agtgacggga
     2761 aggaatcacg gattcttgaa ggcctgttca ggggggtgat cgatgccgag ggccgtgagc
     2821 gtgtgatcat cagtgcccgt gacatcaccg agcgcgagag gaatgtacag gaaattgtga
     2881 ccctggcaag gatctccgcc gaaaatccca atcctgtttt gcggatctcg tccgatggcg
     2941 tcattatcta cgggaaccgg gccagtatcc aggttctgca cgagatcggg gcccagctcg
     3001 gccagacgat acccgatgaa ttccgggcga tcatccggga tgtgatcctg gccggacgga
     3061 atcgtgaggt gcggatgagc ttcggggacc acatctattc catgctcttt gtcccgatag
     3121 tgcccgaggg atatgtcaac ctgtatggaa cggatatcac cgaacggacc cgggccctct
     3181 cagccctcca aaaaagcgaa cgtcagtacc gggccattgt cgaggaccag accgatctga
     3241 tctgccggat gaatccggac tacaaacttt tgtttgttaa tgatgccttc tgccgctttt
     3301 tcaatctctc gcatggagag actgtcggac tcttgtttgc cgggattctc ggcgagaagg
     3361 aagccagacg gtttgcggca ggaattgccg acctgggtgt cgacaatccg gtcctggtca
     3421 tcgagcagga gatcagcgga gacggaggca cacgctggct tgaatggacc tgccgggttc
     3481 tcttcgatct ctcggagagc atcatcgaaa tccagggagt cgggcgcgag atcacggacc
     3541 ggaagatgct ggccgagatg gcccgcgaga aagaggcggc cgagatctcg aatcgcatga
     3601 aaagcgaatt catgtccaat atgagccatg agatccgcac tccgatgaac gggatcctcg
     3661 ggatgacgga gttgatgctc gatactgccc tgacagggga gcagagggac tacgcgttga
     3721 ccatccaccg ctcatcgcag gctctcctga ccatcatcaa tgatattctc gatttttccc
     3781 ggattgaaga gggcaagctc aagattgaag aagcggaatt cgatctggga caggccgtcg
     3841 aggatattgc cagcctcctt gctccgcggg ccagcgagaa gggtctcgaa cttctggtgc
     3901 atttcagccg gcttgcgccg agattggtga tcggcgatca gggccgtgta cggcagatca
     3961 tcctgaatct tgtcaataat gctatcaaat ttacccatga gggctatgtc ctgatccatg
     4021 tcgaccatgt ggccaggcgg gacggttcga atgctttcac gatcgcagtc gaggactccg
     4081 ggatcggtat ctcggcagac aagctggatg tgatctttga acgcttctcg caggcagaca
     4141 catccaccac ccgtttctac ggaggaaccg gcctgggctt gaccatctcc cggcagcttg
     4201 cccagctcat gcacggagat atcgtcgtca acagcagtcc ggggacgggt tcgacattct
     4261 ggtttaccgt gccgcttccg ctttcgccaa acccccaacc ggtttttccg gaccttgccg
     4321 catttcagga gaacgtctca tgctatcttg tggacgccta cgatgtgacc atgcgtgtcc
     4381 atggcgagat tctggctgat ctcggtcttc ctcacattgt cagtttcggg catgaggtct
     4441 gtgattttct caagagggat gatcccgggc ggatacggat tgtgatgatc gacgagcggg
     4501 tggaagatgt tgattgcttt gtcgttgccc gcaaggtccg tgaacttttt tccgatgaca
     4561 aggtcaagct ggttctgctc gccaatcccg agacgatcct cgaggacctg cacgacaagg
     4621 gcgtcgtctt tgacagggtg ctctacaagc ccctcagacc gaggctcttt gccgaacaga
     4681 tgaatgcact catctccggc gagaagtcac ccgcatcgtc ggtccatgtt ccgggaaatg
     4741 tgcggcatga tatccgggaa agcgacctca cggaacccga agcccggagt accgaggtgg
     4801 tgctggatgc ctcgattctc ctggttgagg acaatctggt gaaccagcgt gtggcacagc
     4861 gcatgctcca gaaactcggg tgccgtgtga cgattgccgg gaacggacgg caggctctcg
     4921 agagtctttc cggggggcat tttgatctgg tgttgatgga ttgccagatg cctgtcatgg
     4981 acggttatac cgcgacacgt gaactgagga accctgcttc tccctggtat gctccggatc
     5041 ttccggtcct ggccttgaca gcgcacgccg gggaatccga gcgggagcgc acatttgcag
     5101 cagggatgaa cgaccatatc tccaaacccc tccggagttc ggtcctgctt gagaaactca
     5161 cgacatggct caagacaggc ggtgttcctg aaggggagcc cgggaccgga tcggatgaac
     5221 ctgtccagcc ggaggaggag aagccggctc cggtttttga ccgtgcgggt gcgtatgagc
     5281 ggttgggcga cgatgatggg ctccttcgcg agttgatcca ggtgtttttt ggcgaccttg
     5341 acaaacagct ttcggcgctt cgcggcttcc tcgcggaggg gaatgcgccg gctgcggaaa
     5401 acacgtcgca tgcactcaag ggcggaagtt ccaatatcgg ggctgagcgg attcgccgca
     5461 gattctacga aatggagatc gccggacgcg aagggcggct cgaggatctt cgggctgccc
     5521 ttgaaagcat acccggactg attgccgagc ttgagaacga actgaaggct caaggctatc
     5581 ccgtagaccc ttcctgacgg gtgaagaact caggatttga acagggtggt gtggagcatc
     5641 ctgaggagat tgtcgatacc ctctccggtc ttggccgaga cccggaccag gggctttccg
     5701 gccagttccc gggaggggag accggtctcc atgtcgattt tattggcaca atagagacgg
     5761 gggatccggt ccgcgtcgat ctccgccaga acgctttcga cgacagggat ctcttggtcg
     5821 gcagcatgat cggacgcgtc aaaaacaaca agaaccaggt cggccctggt gatttcttca
     5881 agggtcgagg caaaggcttc cacaagggta tgggggatgt cccggatgaa gccgacggta
     5941 tcgaccaaga gcatggggct cccgttcggg ttatgcagtt ggcggacggt tggatcgagc
     6001 gtggcaaaga gtcggtcttc cgtctggagg tttgtctttg ccagtctgtt catcagggtt
     6061 gtttttccgg cgtttgtgta tccgcagagt gccaccaggg ggatattctt ttcccgtttt
     6121 tttctctgtt cgcggcggct ggttttgact gccgcgaggc gtcggcgtag cagggaaatc
     6181 cggtttttga tgcgcctgcg atccagttcg atttgcgtct cgcccggtcc tctgaatccg
     6241 gatcccccct ggctggcggc aaggtgggca tatccaccct cgagacgtgg caggagatat
     6301 tcgagacggg cgagttccac ctgttgccgg gcgatggctg tgcgtgccct gcgggcaaag
     6361 atttccagga tcagttccgt acgggtcatg accgggcaac cgacggcgtt ctcgaaactc
     6421 cgttttctgg agggagccag atcgtaggag aggatgaaga agtcacaccc ggacgtgttc
     6481 cggatctcct ccagttcggc cagttttccg cttcccggga ggccatgccc cggtcgtgat
     6541 ctggtgctga gcgaaaggtc ggccaggacc tggtagccac aactctcggc gaggaggtgg
     6601 atctcttcgg attctgcagg cagtccctgt gaaattgtga tcacggcagg ggcctcgtgt
     6661 tgatgggtat gcatggttct ccggacaggg ttcattctcc cggtaaatct atggtatgaa
     6721 ctgaaaaaaa ttgacaaaac aagcaaaagg ccgatatagt gaggtgaagt gcagtaattc
     6781 cgtggcaagg tgtgataatg gcagaaatcc ccgataaaag cagacaacag caagtgattc
     6841 tcgatgatga cgacgagatt atctccctgt cagatgccga actcgatacc atcctcggtt
     6901 ccgccgagat cacggatgca ccgatagcgg atatcgagat cgacatcatt tccggtccgg
     6961 catcccgacc ggctccagcc gaagccggaa ggggacagac cggcgatgat actccacaac
     7021 cggaagccgg gggtggcttt ttctcgagcg atgatgatga gacgatttcc ctcccagagg
     7081 atgagcttga gacgatcctc gacaccggaa cctacgaggc cgacggaatg gagccatccc
     7141 ttcccgagag cgaagttgtt gtcgccggtt cagagcaggt gattccggat atgctcgaga
     7201 cgccttccct tggaggcgaa ccggatgtcg agcccgattt tggcgggcct gagacgccga
     7261 ttgatgaggg tgtggacgat gccttcatca ttatcgatga tcaacctctt gagtcttccg
     7321 gactcgggat ggacagcggc ccgatgatca tcgaagagga gatctctccg gatacggcca
     7381 tgcccgagct ggattttgcc cgtgaacagg cagcccgcag caatgatgag ccggtcgtcg
     7441 aagtgaccgg ggactccatg gacgatcttt ttgacgacga tgcggaagtc ccggtggatg
     7501 gtgcgggaga agatgtcctc gacgactttg aggttgagga ccatggtgct gaaatcaatc
     7561 tctccccgcc ccaacctgta ccggttgcgg atattccggg gatagatgcc ctggccgatg
     7621 agccttctcc tctggagata caaccggcac ctcccgagtt tttcctcgaa gagggtgcgg
     7681 ccgagtacga cagcgagagt gtcgacgatc ttctcatcga agaggcgccg atccttgacc
     7741 agcagccggt tgagaggaat gaacttcggg atacgatcat tgatgtcgaa gatgaattga
     7801 tcgatctcga gccgatcgag cgtcgcgagg atcttgagga ctccagccgg agtgaggttg
     7861 tttttgacga tgaggcggaa ggcgataccg tggtatctct tccggtcgga gatggtgcgg
     7921 ccgaagccgg atacgaggca ggcctcgagg aggaaacggt tctcgagacg ggttcgtttg
     7981 tagcggagga tggcattcct cctcccgagc taccggggga ggagcctgtc atcggcgaac
     8041 ctccccgggc ggaagtggcc aaagacgacg gatttgtccg tgcgcccgat tttgaagagg
     8101 agatcgccag cctctcgctc gaggaagcac ctgccgggac ggttccggtc gtggatcagg
     8161 ttgatctggc ggacgaaccg atcgagttca tggaggacga gattgttttt gccccccatc
     8221 aaacgatccc ggcggctgag ccggtttccg acatcgcacc cgatgatgat cttctggacg
     8281 aggagatcat caatcccgac atgatctccg agatgaagga cgaggttttt gacgaaccgg
     8341 attcgcccga tgacgaaccg gttgcttccg atgaagtcag accacccagc caggaggttg
     8401 tggagggcat gcccgagcgg atgcgcgacg atgtgcgcaa ggtgctcgga tacctcgaca
     8461 atctttttga cgaacttccc gaggacaagg tcaaggagtt tgcaaactcc gaatattacg
     8521 atatctacag ccgtcttttc aaggaattgg gtatctgaca gatgggcctg ctctcccgtg
     8581 cacgacacgt cgggtttcgc cggacaccgg cccaggattc cccggcttcc gggcgaaggg
     8641 gtctcctgac acgggccggt atggtccgca gggcccgtgc ccagtctccc gggccggtcg
     8701 acgacccgtc tccggagacg ggttccctcc ggcagcttga gcgacagagc ttcaatctct
     8761 cgaacctgat ggagatcagc cgggagatca acgggacact ccggctggac gatctctgta
     8821 cgatcatcat cctcaccgtc atgggccagt ccggtgccca aaaagcggtt ctctatgtcc
     8881 gggacgacga agaatatgtg gtccgtgccg cacgggggct tgcggagggc ctggttcttc
     8941 cctcgcttcc ggtggatccg gttttcgcac agactgctgt ccaacgggac ggtgtgttcc
     9001 ccgtttcggc ctgtgccttt gttgatggtt cactggagac caggttggtc agtctccttt
     9061 ccgggaaact ggttgtgccc tttgtcaaca agggacagtg cctggccttt ctcctgcttg
     9121 gcgaacgata tcccgaccag gactggaatg aggacgaaat cgggtttctg gcttcgatcg
     9181 ccgatatggg cagtatggcc ctggccaatg cccgactctt cggcgagctt gagaagaagc
     9241 tcggacaact ctccggtctc tacgaaatca gtcgggtcat caatggcagc agcgaggtgg
     9301 gcgagattct ccggctcgcc ggtgagacat tgggaacagg cttcggtgtg aatcgggccg
     9361 catttcttgg cctggctgga cacgatcttg tttttctttc cggcatcggg atcgagggta
     9421 tccgggccgg atcgaccatg acgccccttt ttgaatccgt tttcaccctc gagcaggctc
     9481 tggttattga ggatcgctcc ggaaatcctg aactctccgc tgtctttgga gagggcgtgc
     9541 ttgaagaatc gaatatggca ctcctggtcc ctcttgtcgc ggccggtgag cgcgtgggca
     9601 tcctggccgt cttgggggtg gaggggcgca ggagcgggac cgtcctccag gaggagtgcg
     9661 aactcttttc gattatcggc tcccagtttg ctcctccgct tttggtggcg cggactcttg
     9721 gcgacaggaa acgcagtctg gccgatccat tccatccctg gatggaactc cttgagcgtg
     9781 aagttgcccg tgccggggaa ttcggtcttt ccgtcggagt ctgtgagctc tcgctggacg
     9841 ggatcgacga acgtgacggg gacatggtcc gggatctcgg ccgtgttctg cgggaccgga
     9901 ttgatctgcg ctacccggtg gtccgtctgg cagcagcttc gtgggcggtt gtgctctccg
     9961 ggctttccct gcgggatcag gagcgcttga ttgattccgt gacacaggag atattgactc
    10021 tgcttgaccg ttccggtgat gccgaacgga tcagactcga gcggagccat gggcagatac
    10081 ctgaggaata cccgtcggcg gctgcctggg tgttccgcag gctgtaggcg gccgaccggc
    10141 atgaatctga cagagcagag ggtgcatgag ggtgacatct cgggttcatc cttcagtttt
    10201 ccgggcaggg tcgagatcac gggaagtgtg cgggccggcg ccgttgtgca tgccggtgga
    10261 gatatcgtca tccgcggctc ggtcgaggct ggcctggtcg agagttccgg tggtggcgtc
    10321 tacatcgatg gagagattca tggcccgggg gcagtggtcc gggcctttgg tgatgtcaag
    10381 gcacgtgtga tccgggatgc cgaggtcgag agtctggctt cggtctatgt gcctgacatg
    10441 atcttccggt cggctgtccg ggcccggaac ctgatcgaga tgaaggacgg agacgggatc
    10501 gtcgaggcaa gctatgccga ggcagggatg gagatcacca tccgcagtgc ggggtcggta
    10561 tcaagcggga tggatcccac gacactcgtg ctcgagaact tccgccagaa ggaactcttc
    10621 gagctctcga tgatttatga acagcggctg acccaaaaaa cggcccgcat cgccgagctg
    10681 gaaaaggtga tcagcgtgat ccggattctc gggggccgga tcgtcaatct cgaccctgcg
    10741 aaaaaggcgg atctggcgct caaggtcaag gagtaccagg aactcaagca gcaggttgtg
    10801 gaaattctcc gggaacgtga tctggtcgtg gttgaacggg agaagtctgg cggaaaaatg
    10861 cgctcgatca cgtccagcgg ggatgtcttc tccggtgtcg aggtccggat cgacaatgcc
    10921 cgggttgttg tcaaccggcg ctacaagaat gtcatttttt acaaaagcgg gattgtgatc
    10981 atcggggatc ttgatgcttt tatgcagcgg aaacggcttt ccaattagaa agtcgggcaa
    11041 ggagacggca ggcatgggta tcggattcaa gttgggcatg ttcgtggcag tggttctctc
    11101 actggtcatg ctggtgaccg gggtctttat cgtccgtcag caggagaccg tgctcgacaa
    11161 ccagttgcgc agggatctgt cctcccatct caagtcgttc agcatggccg tgacggccag
    11221 tctttcgggg aaggaagatc tgccccggct ccagtcttcg acctatgctc tctcgaacat
    11281 caacaatttc gggacggcga tgttcgttga tcccagaggc cgggtctttg cccaggttga
    11341 ccggagcggg gtgccttttg acggaagata tgcggttgtc accatgccca ttcccaaact
    11401 ggatgaccct gaactggctg cgcgctatcc cgatatcggc tatcttctct cggatatggt
    11461 ggatggtacg aacatcatat ttgcccggtc cagccggtat acctccgaag aatatgcgca
    11521 agtccttgcc gaaagcgcca gcaacaagac gcctccccct gtcaatacgg cctggttcga
    11581 gggctttctt cccgtctgtg tcaactggct gcagcaggtt catcccggag acaattcgct
    11641 cgacaaggcc ctggctctct accgcgccgg ggtgaccaat gggtttctct tccgcggggt
    11701 gcaaaatgac caacgtcggc gggatctggc ctttctccgt cacatcgaga ccgtggcccg
    11761 ctggctggcc gtcgagaaac ttgatccctg gggaaatcgt gtcggctggg aactctcgcc
    11821 ctatgcgaaa atgctcggtc tcagagagaa ggacaggctg accgagacgg agagctggca
    11881 gatcgccgcc atgattccgt tgattgaggg gttccgcaag ggtgagtccg cgggttcgcc
    11941 cgaaaaactc tttccccggg agaccatcac ccggatcacg agtcttgtcg aacggtattt
    12001 ttcgggcgca gcgatgcccg acgggttggg ggggcttttg acggatgcca gggccgggaa
    12061 actcctgatg gccggtgttt cgcccggaga agaggccgag atcaggatgg ctgcggattg
    12121 gctcgaggtc gcccgggcgc tgaccaggtc gagcggaccg gagtggaaca aggtctacgg
    12181 cgagatggcc gctgcccgcc agatagcgcc ctttgtacat ggagacgaga tcttcgtgga
    12241 tattgccttg ttcgccggac tgtttgaagc ctatctgacg ggcggggaat tccccatgcc
    12301 cgagacccat cgctatcgca aactctccag gatctatgaa cccaaaaaga gtcccgaaga
    12361 gcg
//
LOCUS       DAOSHE010000012        12300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4331_length_12300_cov_4.582115, whole genome shotgun sequence.
ACCESSION   DAOSHE010000012 DAOSHE010000000
VERSION     DAOSHE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4331_length_12300_cov_4.582115"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLD82_00795"
     CDS             complement(<1..920)
                     /locus_tag="PLD82_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63956.1"
                     /translation="MKHVLLFLVLSLCTHPMMASATDLGTLILSIPGSEVPSQALPDR
                     LVAIPNDLFTEATGFRYEAIPGIRSRTPGKPEDGLVYWIPKEWVDALSLRIHRENALE
                     TGMRAGETMRIYSAPRFSGLSADLNEIVQQTLTVSLAYRASGYDTRLLDTLYLTWQGA
                     FLQTLPAYQAMHASQDQRPDPAAIDALLDRLNAPDQIASTKPEMRLAVFNRHLFSGLL
                     RMSRAILAERPGSQSAWKCLVMLARSRNNAALLEEALRALKRLAPENPEQTMRLAEAL
                     YEAGSHECTNLAAKAMDMNPKLSHHAQAILR"
     gene            1042..1209
                     /locus_tag="PLD82_00800"
     CDS             1042..1209
                     /locus_tag="PLD82_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63957.1"
                     /translation="MDVSGVAVQQQVQLTVLRKTLDMVANQARMVMEQGAAASQAIAA
                     QSGAHQVDIKV"
     gene            complement(1273..3798)
                     /locus_tag="PLD82_00805"
     CDS             complement(1273..3798)
                     /locus_tag="PLD82_00805"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG63958.1"
                     /translation="MTMPNDIDREEKGNRPSLWQDYHKGMDVDSIILSFKNHMKYTMA
                     KDHYTATDWDHFYSMSRVIMDRLIERWIGTQQTYYNTDAKRVYYLSLEFLVGRLLGNN
                     LINLMMMDKARAALHRMSLDMEDLRNVEVDAGLGNGGLGRLAACFLDSMATLELPGYG
                     YGIRYEFGIFRQIIRDGFQIEVPDEWLRFGNPWEIARPESRFEVKFGGTVENRPTRNN
                     VINPVWVNYNTVLGVPYDTPVPGYNNNTVNTLRLWSAKSSEEFDLEIFNDGDYVSAVE
                     NKNLSEVISKVLYPNDNLLSGKELRFKQEYFFVSCSLQDIVRRFKKQHGNHFERFPDK
                     VAVQLNDTHPALGIAELMRILIDEEKIPYEKAWEITQGSFAYTNHTLLPEALEKWPVP
                     IFEKILPRHIQIIYEINRRFMRDVASRFFNDRHKQSSLSLIEEGPEKQVRMAHLAVLG
                     SHSVNGVAALHTELLKSNVLHDFYEYCPEKFNNKTNGITQRRWLLKANPGLASLISDH
                     IGTGWITNLDELRKLEPFADDPAFQQRFADIKRTNKAYLCKLIEKETTIPVQPDALFD
                     IQVKRIHEYKRQLLFALGIIIQYIRLKKNPSLDIAPRVYICGGKSAPGYHMAKLIIKL
                     INSISEVVNNDSLIGNKIKVVFFPNYRVSVAEKIFPAADVSEQISTAGMEASGTGNMK
                     FAMNGALTIGTLDGANIEIREEVGAENFFLFGKTVEELEKLSAEGYNPRHYYENNPEI
                     REALDLLATDFFCGGQPDLFRPILDALLVHGDRYFNLADLEDYLATQDRIDRTYRDQK
                     VWNRMAILNVARMGKFSSDRTILEYANEIWGVKPTSIAVTTAE"
     gene            complement(3847..4368)
                     /gene="dcd"
                     /locus_tag="PLD82_00810"
     CDS             complement(3847..4368)
                     /gene="dcd"
                     /locus_tag="PLD82_00810"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOG63959.1"
                     /translation="MILSGIKIQEERARGRIAIDPWDPEQLNPNSYNLTLSDEIMHYR
                     DTELDMKRKPEPVLLRIPPEGLVLQPGKLYLGRTAEYTETHGYVPMIEGRSSIGRLGM
                     FVHITAGFGDVGFAGFWTLEIMVLQPTRVYSGVQICQIFYHDISEAHDEYRSGKYQNN
                     TGIQESMLYRDFL"
     gene            4466..5629
                     /locus_tag="PLD82_00815"
     CDS             4466..5629
                     /locus_tag="PLD82_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63960.1"
                     /translation="MIVVALGLAQGVFGSGGYDAATIRYIKEHLDKPGHTWSGYQGVA
                     RGLASTNREVRFFAVQAGLQLDVFRSEALGMALKDTDPGILNLGLAALEREYRTQYSP
                     RLVEIVLSGHVAGLPALRLLLRHDLPHVALSFETLFQRGGRAVRESLLEELRKGRIVM
                     SAPAILALSDLMGTRERVMVLACLAQRGEAAFFAGEYGRWSADVRRKFIQALRDDAQM
                     AAEPALALLLGHCRDSGEEEALRSALLAAGNQGLRQVVARELLEARDMAAARALADAL
                     VVLGGKEDQALEYARAVIMIDRYAMGERILAGWAGQRLKGLSELATSLRNPGTMGAVR
                     TIGRLQEDARALQRITREQAEMRAKVRSYLQSPASVHLKRNLAAFLASAKLSF"
     gene            complement(5648..6157)
                     /locus_tag="PLD82_00820"
     CDS             complement(5648..6157)
                     /locus_tag="PLD82_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG63961.1"
                     /translation="MQSGSYQYAWLNNIYIIKLSGSLTYSSSGDLDAFISRIFAERNF
                     SNILIDLSETDSLDSTNLGLLAKIGRYTLDTFQRKTSMISPRDDINRVLRSMGFDSVF
                     TIITEPEHFSGTLSSIPHTEQSERETAQTILDAHRTLMEMNDANRSVFKNVVKFLEKE
                     IDSRDANAH"
     gene            complement(6165..8138)
                     /locus_tag="PLD82_00825"
     CDS             complement(6165..8138)
                     /locus_tag="PLD82_00825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG63962.1"
                     /translation="MHTRIRSVLALVLIWTCTTVVTADSAQVRPLRILVVQSYNASML
                     WSQAVQRGIEETIEKEAPESHIESFYMDTKFHFSPEYMSRLADLFRFKYTGQLLDAVI
                     TVDDAALAFLLELRPSVMPATPLAYCGVNRLSNDRPAGHEGVLGIVEDIDIASTIDLA
                     RSLHPGLRTVYVINDRTMTGLENRRMLNALTPRWPSLRFVYFDTLSWNGLSQAVAAIP
                     DASIILLLTHTTDAAGVSMSYRESIRKIRAVARVPIYGVWDFYLGRGLLGGMITSGFD
                     QGRRAALLALSMVQGNPADQLVSIRKSPNQPVFDAAEMARFRISRDRLPEGSTVINLP
                     PSFVRENFPVVVAALSFILIVTIVIALLLVSIRRRKNAESSLRESESRYRTLSRELEE
                     TVALRTNELKHRNLEMQQLLDELGRDAQAGQRIQFQLLPETPYHAGEYRIDHLLHPSR
                     YLSGDFLDYFEIDADHTGLYMADVSGHGLPSAFVTVFLKSLIDQALEKYRSGSSRIIT
                     NPARLLLHLNGEILRQHIDKHLTIFYGVLERSTNRLTWANGGQFPFPVLSTPSGTSFI
                     EQNSHPVGLFGFADYTNVIFELPEVFSLTLASDGILEIINENNLGEKERKLLQAADRT
                     KGEISPVSILRRLDFDEGRALPDDITILVLTRE"
     gene            8304..9812
                     /locus_tag="PLD82_00830"
     CDS             8304..9812
                     /locus_tag="PLD82_00830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOG63963.1"
                     /translation="MAKMLEILKTRFGYPGFKAGQEEVVRDVLAGGHRLVVLPTGGGK
                     SLCYVLPAVLRAGKTLVVSPLIALMEDQAERAASVGLRALVWRRETTRDELLQAGMIF
                     ASPEKLMHQAGPWLGRLGIWHLVIDEAHCVEDWGGSFRPMYTQLAGLQSWIEPYSLSV
                     FTASAGEEQRASLVRRLGLRDARCFVHGFDRPNIAWHSHQTAFPLLAALHILRKTEGR
                     AIVYCRTRLGASRSAAVMQASGMDARPFHAGMRRDVRMHLQREFLEGRIRILCATSAF
                     GMGVDVPAVRTVIHLGLPSGRNAYYQESGRAGRDGQSALAVLLTQPGEQVRLDAVERR
                     NRVLLALIRQAGDGWTQDGFASRLRVLGGGQEPAEMAAFLVHHGICRMERGGSIIPLI
                     GAGERVRMAIREDMERRAEESRWIGDFVSADGGCRRKHLVGGFEDVSGLVCHGCDACG
                     SEYSEMLARILGQTATDAEVVRNFVKLPWLERPALVFGEQISVLREEWSARK"
     gene            complement(9816..10175)
                     /locus_tag="PLD82_00835"
     CDS             complement(9816..10175)
                     /locus_tag="PLD82_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG63964.1"
                     /translation="MQDKTADIQHEDIQGVRVIELCTRIDIHNVFAVEETLTQLIDEG
                     HTKVIIDFSRIEYFGSNGIRLLMLAKRKLDHLGGRLVLSSMSSFVVKILKAIDLYELF
                     TILPDRASALEELQTNR"
     gene            10391..11023
                     /locus_tag="PLD82_00840"
     CDS             10391..11023
                     /locus_tag="PLD82_00840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG63965.1"
                     /translation="MKQRTIDILYEGEDWVIINKPAGLPVQPGEAVSANLVDELTGIW
                     GYPPHLVHRLDRDTGGCLVVGRTGKAAARLSKILADPASRKTYLAVVKGVPTDPSGEI
                     DETIEVHGVKKRAVTRWKLVRPLGGLCSLLEIGLDTGRMHQIRIHLAKNGHPLAGDDR
                     HGDFPFNRLLKKEAGVKKLLLFAVRLELAGGIRVEAPWPAHLRAFCERYP"
     gene            11093..>12300
                     /locus_tag="PLD82_00845"
     CDS             11093..>12300
                     /locus_tag="PLD82_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63966.1"
                     /translation="MIVVVIGAMALVSLIGMTLWRHFGLSLKLSRFSSMVYHGQAAEA
                     LHGLRLLENRHHGDIRVHQLLGQGLMVAGEYGQAAREWEIVHLINRHERVLALAEIAG
                     PLADCQMALGEWRDAQGGLLLALKACPGEVGLLERLAMVYLKRGMARHAVSCYENILK
                     LEGEEIVTILKMATVQEIAGDLRGALASALHVLRLDAKNGPAALMAARLYAGFGNHAS
                     AEPIWRGLAARKEYRGEGLTGLAACLEAQPGRTEDTVDAWLEVLQVTETKTERLEVLY
                     RMASLLLQSGDIRKGVLLLEEIVNLEPGYRDAARQLERYGDLYAGGDIGRFATGSIEE
                     FRPVSGKILDAFGITVLRDKVTRKRDLVVHGVVGKEGKRETVLVHFSRSLSALGERDL
                     QEVADEMRVLR"
BASE COUNT         2484 a         3004 c         3949 g         2863 t
ORIGIN      
        1 cgcagaatgg cctgtgcatg gtgcgaaagt ttcgggttca tgtccatggc tttggcggca
       61 aggtttgtgc actcatggga accggcctca tagagagctt cggccagtct catggtctgt
      121 tcggggtttt cgggggcaag ccgttttagc gcgcgcaggg cttcttcaag cagtgctgca
      181 ttgttcctcg agcgggccag catgacgaga cacttccatg ccgattggct cccggggcgc
      241 tctgcaagga tggcccggga cattcggagc aagcccgaaa aaagatgtct gttgaatacg
      301 gcgagacgca tttccggctt ggtggaggcg atctggtccg gagcgttcag ccgatccagt
      361 aaagcatcaa tggcagccgg atcggggcgt tggtcctgac tggcgtgcat ggcttggtac
      421 gcggggagcg tttgcaggaa cgcgccttgc caagtcagat agagtgtatc caacaagcgg
      481 gtgtcgtatc cgctggcacg ataggccaga ctgacagtga gtgtctgttg gacgatttcg
      541 ttcagatctg ccgagagtcc gctgaagcgg ggtgcactgt agatgcgcat ggtttcgccc
      601 gctctcatgc cggtctcaag agcattctcc cgatggatgc gcagggacag ggcgtccacc
      661 cattccttgg ggatccagta gaccagccca tcctcgggtt ttccgggagt gcgggatcgt
      721 attccgggga tggcttcata gcggaagccg gttgcttcgg taaaaagatc gttcgggatg
      781 gcgacaagac ggtcgggtag ggcttgtgac ggcacttctg atcccggaat ggagaggatc
      841 agggtgccga gatccgttgc cgatgccatc attggatgtg tgcagaggga aagaaccaga
      901 aaaagaagca cgtgtttcat gatcgggaca gaacctccgg gttgtcgctg atgttccctt
      961 aatgataccc cccttcgggt aaataatcgc ccatcgcttg ccgataagag atgtataggt
     1021 cgttgttatg gaggtcatgt catggatgtc agcggtgttg cggttcaaca acaggttcaa
     1081 ttgacggttt tgcgcaagac gctcgatatg gttgccaatc aagccaggat ggtgatggaa
     1141 cagggtgctg cagccagtca ggccattgcg gcccaatctg gggcgcatca ggtggatatc
     1201 aaggtctgaa gagacgtctt ccccggatag agagatacaa aagaccccgc gcgagcgggg
     1261 ttcctttgtt tgttattctg ctgttgtcac cgcaatcgaa gttggcttga caccccagat
     1321 ctcgttcgca tattcaagga tggtccggtc actcgagaat tttcccatcc gggccacatt
     1381 cagaatagcc atcctgttcc agaccttttg gtccctgtag gtgcggtcga ttctgtcctg
     1441 ggtggcgaga tagtcctcga gatccgccag gttgaaatag cggtctccgt gaacgaggag
     1501 agcgtccaga atcgggcgga aaagatccgg ctggccgccg cagaaaaagt ctgtcgcaag
     1561 aagatcgagc gcctcacgga tttcgggatt gttctcgtaa tagtggcggg ggttgtatcc
     1621 ttcggcactg agcttttcga gttcttcgac cgttttcccg aagaggaaga agttctctgc
     1681 accgacctct tccctgattt cgatattggc tccgtccaga gtcccgattg tcagggcgcc
     1741 gttcatcgcg aatttcatgt ttccggtgcc tgaagcctcc atcccggcgg tggagatctg
     1801 ttcgctgaca tcggcggccg ggaaaatctt ttcggcgacc gagacccggt aattcgggaa
     1861 gaagaccact ttgatcttgt tcccgatcag gctgtcattg ttgacgactt cgctgatcga
     1921 gttgatgagt ttgatgatca gttttgccat gtggtagcct ggagccgatt tgccaccgca
     1981 gatgtagacg cggggtgcga tatcgaggga gggattcttt ttcaggcgga tgtactggat
     2041 gatgatgccc agggcaaaga ggagttgtcg tttgtactcg tgaatccgct tgacctggat
     2101 gtcaaagagg gcatctggct ggaccgggat ggtcgtttcc ttctcgatga gcttgcagag
     2161 gtatgccttg tttgtccgct tgatgtccgc gaagcgctgt tggaaagccg gatcgtcggc
     2221 aaagggctcg agtttgcgga gctcgtcgag attggtaatc cagccggttc cgatatggtc
     2281 gctgatcaga cttgccaggc ccggattggc tttcaggagc cagcggcgct gtgtgatccc
     2341 gtttgtcttg ttgttgaatt tttccgggca gtattcgtaa aaatcgtgca ggacattgct
     2401 cttgaggagt tccgtgtgca gtgctgcgac gccgttgacg gaatgggagc cgagaacggc
     2461 caggtgtgcc atccggacct gcttttcggg cccttcctcg atcagggaga ggctggactg
     2521 cttgtggcga tcattgaaga agcggctggc aacatcccgc atgaagcggc ggttgatctc
     2581 gtagatgatc tggatatggc gtggcaggat cttctcgaag atcggaacgg gccatttttc
     2641 gagagcttcc ggcaggaggg tgtggttggt ataggcgaag gatccctggg tgatctccca
     2701 tgccttctcg tatggaatct tctcttcgtc aatcaggatg cgcatcagtt cggcaattcc
     2761 gagagcgggg tgggtatcgt tgagctggac agcaaccttg tccgggaacc gctcgaagtg
     2821 gtttccatgc tgttttttga aacggcgcac gatgtcctga agggagcagg agacaaagaa
     2881 gtattcctgt ttgaagcgga gttccttccc cgagagaaga ttgtcattcg ggtagagaac
     2941 cttggagatg acctcggaca ggttcttgtt ctcgacggcc gagacgtaat caccgtcgtt
     3001 gaagatctcg agatcgaatt cttcggaact cttggcgctc cagagacgga gtgtgttgac
     3061 cgtgttgttg ttgtagccgg ggaccggagt gtcgtaggga acgccgagca ccgtgttgta
     3121 gttgacccag accggattga tgacattgtt tctggttggc cggttttcaa ccgtaccgcc
     3181 gaacttgacc tcgaagcgtg attcaggccg tgcgatctcc caggggttgc cgaatcgcaa
     3241 ccattcgtcc gggacctcga tctgaaaccc gtctcggatg atttgtctga agatcccgaa
     3301 ttcatagcgg attccgtatc cgtagccggg gagttcgagg gtggccatgg aatcgaggaa
     3361 acacgcggcg agccggccca gaccgccgtt accgagaccc gcatcaactt cgacgttgcg
     3421 cagatcctcc atgtcaagag acatgcggtg caacgcagcc cgggccttgt ccatcatcat
     3481 caggttgatc aggttgtttc caagcaggcg cccgacaagg aattcgaggg aaagatagta
     3541 aacgcgtttg gcgtcggtgt tgtaataggt ttgttgtgtg ccgatccatc gttcaatcag
     3601 gcgatccatg atcacacggg acatgctgta aaagtggtcc cagtcggtgg ccgtgtaatg
     3661 gtccttggcc atggtgtact tcatgtggtt tttgaaggag agaatgatcg aatcgacatc
     3721 catccccttg tggtagtctt gccagagaga agggcgattg cctttttctt ctcggtcaat
     3781 gtcgtttggc atggtcatga aaggtccttg cgatgtcaga aatggcgtac cggccaacgg
     3841 ccggactcag aggaaatccc ggtagagcat gctctcctgg atcccggtgt tgttctggta
     3901 tttgcctgaa cggtactcgt cgtgcgcctc gctgatgtca tggtagaaaa tctggcagat
     3961 ctggactccc gagtacactc tggttggctg caaaaccatg atttcaaggg tccagaatcc
     4021 ggcaaaaccg acatccccga atccggcggt gatatggacg aacatgccga gacgaccgat
     4081 ggaagaccgc ccctcgatca tcgggacgta tccgtgtgtc tccgtgtatt ccgccgtccg
     4141 tccgagatag agcttgcccg gttggaggac gagcccttca gggggaatcc gcaacaatac
     4201 aggctcgggt ttgcgcttca tgtcaagctc ggtgtcccgg tagtgcatga tttcatcgga
     4261 gagcgtcaaa ttgtaggaat tcggattgag ttgttcaggg tcccatggat caatggcgat
     4321 acggccacgg gcccgctctt cctgtatctt gattccggaa aggatcatgg tcgtgtctcc
     4381 aaagatttat taatgttttg taccagaaaa aaagataatc aagaagatga cgataaaaaa
     4441 atctcacaat tttccgttcg tgctcatgat agttgtggca cttggcttgg cgcaaggggt
     4501 attcggttcc ggcgggtatg atgcggcaac aatccggtat atcaaggagc atcttgacaa
     4561 acccgggcat acctggtccg ggtatcaggg tgtggcccga ggtcttgcga gcaccaaccg
     4621 tgaggtcagg ttttttgcgg ttcaggcagg gctccagctc gacgttttcc ggtcggaagc
     4681 cttgggcatg gctctcaagg ataccgatcc cggaatcctc aaccttgggc tggcggccct
     4741 tgagcgggag tacaggacac agtacagtcc caggctcgtc gagatcgtcc tttccggaca
     4801 cgtcgccggg cttcctgcct tgcgcttgct tctcaggcac gatcttcctc atgttgcgct
     4861 ttctttcgag acgctgttcc agagaggtgg ccgtgccgtc agagagagtc tccttgaaga
     4921 gttgcgcaaa ggacggatcg tcatgagtgc gccggcgatc cttgccttgt ccgatctgat
     4981 gggcacccgt gagcgggtga tggttctggc ctgtctggca caacggggtg aggcagcttt
     5041 ttttgccggg gaatacggac gctggagtgc cgatgtccgg cggaaattta tccaggcgct
     5101 ccgtgatgat gcacagatgg ctgccgagcc ggctttggcg cttcttctgg ggcactgccg
     5161 ggattccggg gaggaggagg ccctgcggtc tgcccttctc gctgccggga atcagggctt
     5221 acgccaggtg gtggcgaggg agcttcttga ggcgagggac atggccgctg cccgggcctt
     5281 ggccgatgcg ctggttgtcc ttggaggaaa agaggaccag gcactggagt atgccagggc
     5341 cgtgatcatg atcgaccgat acgccatggg tgaaaggatt cttgccggtt gggccgggca
     5401 acgactcaag ggcctttcgg aattggcgac gagtctcagg aatcctggaa cgatgggcgc
     5461 cgtacggacc atcggtcggc tccaggagga cgcgcgagcc cttcagcgta ttaccaggga
     5521 gcaggccgag atgagagcca aggtcaggag ctatctgcag tcgccagcat cagtgcatct
     5581 caagcggaat ctcgcggcct ttttggcgtc ggcaaaactg agtttttaag gcgcgaccgg
     5641 ttctgtctca gtgtgcgttt gcatcacggg agtcgatctc tttttcgagg aacttgacga
     5701 catttttgaa gacggagcgg ttggcgtcat tcatttccat cagggtgcgg tgcgcatcca
     5761 ggatggtctg cgccgtctcg cgttcgctct gttctgtatg ggggatggac gagagggttc
     5821 cggaaaagtg ttccggctcg gtgatgatcg tgaagacact gtcaaaaccc atgcttctca
     5881 agaccctgtt gatatcgtcc cggggggaga tcatggatgt ttttctctgg aaggtatcga
     5941 gggtgtatct tccgattttg gccaggagtc cgagattggt actgtccagg ctgtctgtct
     6001 ccgagagatc gatcaggata tttgaaaaat ttctttccgc gaaaatcctg ctgatgaaag
     6061 cgtccagatc tccgctggag ctgtaggtca ggcttccgct gagtttgatg atgtagatgt
     6121 tgttgagcca ggcgtactgg taggaaccac tctgcattgg atactcactc ccttgtcagg
     6181 acaagaatgg ttatgtcgtc gggaagtgct cttccttcat caaaatctaa tcgccggagt
     6241 atggatacag gggaaatttc tcccttggtc cggtcggcgg cctgcaggag ttttcgctcc
     6301 ttttcgccaa gattgttttc gttgatgatc tcgagaatcc cgtctgacgc cagcgtaagc
     6361 gaaaagactt ccgggagttc gaagatgaca ttcgtgtaat cggcaaaacc gaagagaccc
     6421 acaggatggc tgttttgctc gataaagctg gtaccggatg gagtggagag tacgggaaag
     6481 gggaattgtc caccgttggc ccaggtcagg cggtttgtgc tccgctcgag aactccgtaa
     6541 aaaatcgtga ggtgcttgtc gatgtgttgc cggagaattt caccgttgag atgcaggagg
     6601 agccgtgccg gatttgtgat gatgcgactg ctcccgctcc ggtatttttc gagtgcctgg
     6661 tcgatcaggc tctttaaaaa gacagtgacg aaagcggatg gcagtccgtg tccggaaaca
     6721 tcggccatgt agagtccggt gtggtcggca tcaatctcga agtaatccag gaaatctccg
     6781 gagaggtagc gtgaaggatg gagaaggtga tcgatacggt attctccggc gtggtagggc
     6841 gtctcgggca agagctgaaa ctggatgcgt tggcctgcct gggcatccct gcccagttca
     6901 tcaagaagct gctgcatctc gaggttccgg tgtttaagtt cgttcgtgcg gagggcgacg
     6961 gtctcctcga gttccctgga gagggttctg tagcgggatt cactttcccg taaactcgac
     7021 tccgcgtttt ttctgcgacg gatactgacg aggaggagtg cgatcacgat ggtgacgatg
     7081 agaatgaagg agagcgccgc aacaacgacc ggaaaattct cccggacaaa ggacggagga
     7141 aggttgatga ccgtgctccc ttcgggcaga cgatcccggg aaatccggaa acgcgccatt
     7201 tcggcagcat cgaagacagg ttggttgggg ctcttccgga tgcttacaag ctggtcagca
     7261 ggatttccct gcaccatgga aagggccaga agtgcggctc tgcgtccttg atcaaacccg
     7321 ctggtgatca ttccgccgag gagtccgcgc ccgagataga aatcccagac cccatagatc
     7381 ggaacccggg ctacggcacg gattttgcgg atactttcac ggtagctcat gctgaccccg
     7441 gctgcgtcgg tggtatgggt caagaggagg atgatgcttg cgtcggggat ggcagcgaca
     7501 gcctgggaga gaccattcca ggagagggta tcgaaataga caaacctcag actgggccag
     7561 cgcggggtga gcgcgttgag catccgacgg ttttccaagc cagtcatggt ccggtcattg
     7621 atgacgtaga ctgtccgcag accgggatgg agggagcggg ccagatcgat tgttgacgca
     7681 atgtcgatat cctcgacgat gccgagcaca ccttcgtggc cagccgggcg atcgttgctg
     7741 agccggttga caccgcagta ggcgagtggt gtggccggca tgacagaggg gcggagttca
     7801 aggaggaagg ccagggcggc atcgtcgacg gtgatcacgg catccagtaa ctgccccgtg
     7861 tatttgaagc ggaagagatc ggcgagtctg gacatgtatt cgggtgaaaa atggaatttt
     7921 gtgtccatgt agaaagactc gatgtgcgat tccggcgctt ctttttcaat cgtttcttcg
     7981 atgccgcgct ggaccgcttg ggaccagagc atggatgcgt tataggattg gacgaccagg
     8041 atacgcaaag gtcggacctg tgcggaatct gcggtcacga cagtggtaca ggtccagatc
     8101 aggacgagag ccagaacaga tcgaattcgg gtatgcatcc ttgaatgatg ccacaaagcg
     8161 cgctcgcttg tcaaattcgc caatttcgaa aattgtcggt ctgtgggccc cgggtttccg
     8221 gttttgtacg gtttttcagc aaaaacaggg aacccgggat gttcgtcgga cctcttctgt
     8281 atatgcgaga gacaataccg gaaatggcaa agatgctgga aatcctgaaa acccgttttg
     8341 gctatccggg attcaaggcc gggcaggaag aggttgtgcg ggatgttctc gccggggggc
     8401 acaggcttgt cgttctcccg acagggggcg gaaaatctct gtgctacgtt cttccggcgg
     8461 ttttgcgggc aggaaagacg cttgttgtca gtccgctgat tgccttgatg gaggatcagg
     8521 ctgaacgggc tgcgtcagtc gggctccggg ctcttgtctg gaggagggag accaccaggg
     8581 atgaactctt gcaagccggg atgatctttg ccagtcccga aaagctgatg caccaggcgg
     8641 gtccctggct cggccgactg ggtatctggc atctcgtgat tgacgaggcc cattgtgtcg
     8701 aggactgggg cgggagcttc cggccgatgt acacccaatt ggcagggttg cagtcctgga
     8761 tcgagccgta ctccctgtcg gtttttaccg ccagtgccgg tgaagaacag cgcgcttcat
     8821 tggtcaggcg gcttggtctg cgggatgccc gctgtttcgt tcacggattt gaccggccga
     8881 acatcgcctg gcacagccat caaacagcct tcccgctgct ggcggcgctt catatcctgc
     8941 ggaagacaga agggcgtgcg atcgtgtatt gcaggacacg tctcggcgca tcccggagtg
     9001 ctgccgtgat gcaggccagc gggatggatg ctcgcccgtt ccatgccggg atgagacggg
     9061 atgtgaggat gcatcttcaa agagaatttc ttgaagggcg aatcaggatt ctctgtgcga
     9121 cgagtgcttt cgggatgggt gtggatgttc ctgctgtgcg gaccgtgatt catcttggct
     9181 tgccgtcagg gcggaatgcc tactatcagg agtccggccg ggcgggtcgg gatggccagt
     9241 ctgccttggc cgttctttta acacaacccg gtgaacaggt caggctggat gcggtcgaga
     9301 gacggaacag ggttctcctg gccctcattc gccaggcggg ggatgggtgg acccaggatg
     9361 gttttgcgtc gaggctcaga gttttgggcg gagggcagga accggcagag atggcagcat
     9421 tccttgtgca ccacgggatc tgccggatgg agaggggcgg gagcatcatt cctctgatcg
     9481 gcgccgggga gagggtgcgc atggcgatca gggaagacat ggaacgacgg gcggaggaga
     9541 gccgctggat cggagatttc gtttctgccg atggcggttg cagaagaaag catttggttg
     9601 gagggtttga ggatgtatcc gggctggtgt gccatgggtg cgatgcctgt ggctcggagt
     9661 attcggagat gctcgccagg attctcgggc aaacagcgac cgacgcggaa gtggtccgga
     9721 atttcgtaaa acttccgtgg cttgaacggc cggcgcttgt tttcggggaa cagatctcgg
     9781 tcctgcggga ggagtggagt gcgcggaaat agggtttagc ggttggtttg taattcctcg
     9841 agagccgagg cccggtcggg aagaatggtg aagagctcgt agaggtcgat tgctttgagg
     9901 atcttgacga cgaaactgct catgctggag agaaccagcc tgcctccgag gtgatccagc
     9961 ttgcgcttcg caagcatcag gagacggatt ccgttgctgc caaagtactc gatccggctg
    10021 aagtcgatga tgacctttgt gtggccttcg tcgatgagct gggtcagtgt ctcctcgacg
    10081 gcaaacacgt tatggatgtc tatccgcgtg cagagctcga tgacccgcac tccctgtatg
    10141 tcctcatgct gtatgtcagc cgttttgtcc tgcatgtccc tgctgctcca tccaatgctt
    10201 gtgccccgcc gcggatggcg gaaccacagt gtaagaatac accgtttttg ccggaatacg
    10261 caagcgtaaa cggcggggcc ggcccccttg cggtaaaacc ggtgaatgat caccagttcc
    10321 gggaaggtat atcctgtaca gggaccttcg gctgttcctc agactatctt tagaacagaa
    10381 cgggggggct atgaaacaac ggacaatcga catcctgtac gagggtgagg actgggtgat
    10441 catcaacaaa ccggcaggtt tgcccgttca acccggagag gccgtgagtg cgaatctcgt
    10501 tgatgaattg accgggattt ggggatatcc accacatctg gtgcaccgcc tcgaccggga
    10561 taccggcggg tgtctggttg tgggcaggac cggaaaggcg gcggcccggc tttcaaagat
    10621 tctggctgat cccgccagcc gcaagaccta cctcgcggtt gtcaagggtg tgccgacgga
    10681 tccatcaggc gagatcgacg agacgattga agtccatggt gtcaaaaagc gggctgtgac
    10741 ccgttggaaa ctggtgcgac ccctgggagg gctctgttcc ctgctggaga tcgggttgga
    10801 tacggggcgg atgcatcaga tccggattca tttggccaaa aatggccatc cgctggccgg
    10861 agatgatcgc catggtgact ttccgttcaa tcgtctgctg aaaaaggagg ctggggtcaa
    10921 aaaacttttg ctcttcgccg tccgtctcga actggcgggt ggaatcaggg tggaagcacc
    10981 atggccagcc cacttgcggg cattctgcga acgctatcca taaaccgttt ttgctttcct
    11041 gccgatcatg agaccagaga tatggctcat gccggacgcg ggagggcgga cgatgattgt
    11101 ggttgtgatt ggtgctatgg cgctcgtttc cctgatagga atgacactct ggagacattt
    11161 cgggctctcc ctcaaacttt cacggttttc atccatggtg taccatggtc aagcggcgga
    11221 agccctgcac ggcctgcgac ttttggagaa caggcaccat ggggacatcc gggtacacca
    11281 actcctgggt caaggtttga tggttgccgg agaatacggt caggctgccc gggagtggga
    11341 gatcgtgcat ttgatcaatc ggcatgagcg ggtattggct ctggcggaga ttgccggccc
    11401 tcttgctgac tgccagatgg cgctcggtga gtggcgggat gcccagggag ggctgcttct
    11461 ggcgctcaag gcctgtcctg gcgaggtcgg tcttctggaa cgcctcgcca tggtgtacct
    11521 caagcgcggt atggcccggc atgctgtttc ctgctacgag aacattctca aactcgaggg
    11581 tgaagagatt gtgacgatcc tcaaaatggc gacggtgcag gagattgccg gcgatttgag
    11641 gggggcgttg gcgagcgcgc tccatgtctt gcggctggat gcgaaaaatg gtccggcagc
    11701 gctgatggca gcccggctct acgccggatt tggcaatcat gcttctgcag aacccatctg
    11761 gcgtggtttg gccgccagga aggagtatcg cggggagggc ctgaccgggc tggcagcctg
    11821 tcttgaagcg cagccgggaa ggacggaaga taccgttgat gcctggttgg aagtcctcca
    11881 ggtgacggag acaaagaccg aaaggctgga ggttctctac cgcatggcaa gtcttctgct
    11941 tcaatccgga gatatccgga agggagtcct ccttcttgag gagatcgtca atctggaacc
    12001 cggataccgg gatgctgccc gacaactgga acgctatggt gatttgtatg caggggggga
    12061 tatcgggcgc tttgcgaccg ggagcatcga ggaattccgg cccgtttccg ggaaaatcct
    12121 tgatgcattc gggatcaccg ttttgcgtga caaagtcacg cggaagcggg atcttgtggt
    12181 ccatggtgtg gtgggtaagg aaggcaagcg ggagacggtc ttggtccatt tctcgaggag
    12241 cctttccgct ctcggggaga gggatcttca ggaggttgcc gacgagatgc gggttcttcg
//
LOCUS       DAOSHE010000013        11317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4846_length_11317_cov_4.757858, whole genome shotgun sequence.
ACCESSION   DAOSHE010000013 DAOSHE010000000
VERSION     DAOSHE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_11317_cov_4.757858"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLD82_00850"
     CDS             complement(<1..921)
                     /locus_tag="PLD82_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOG63967.1"
                     /translation="MAELTASVDGVLTSFCPAGSANDPALAEAVIFLPGQSAADELGK
                     RLVTLQASNLVENGDRTFELHHGGKLGIESRTALSGPDELAMAYTPGVARVCKHIESD
                     AEAAWDLTIKGTTVAVITDGTAVLGLGDIGTLAALPVMEGKAALFKVFGGVNAFPVCL
                     DTRDPDEIVETVARIALVFGGINLEDISAPRCFEIEKRLDGMLDIPVFHDDQHGTAIV
                     ALAALENAVCITGRKMESLKIVVSGAGAAGIAVAGILRGRGVGEIILCDSKGIVSIDR
                     NDLNGYKQAFAVSRGGSLADALRVADVFVGV"
     gene            complement(1036..1695)
                     /locus_tag="PLD82_00855"
     CDS             complement(1036..1695)
                     /locus_tag="PLD82_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63968.1"
                     /translation="MIPMPPPPDGGVTLLEIVGYVSSVLIAVSLMMSNIYKLRVINFI
                     GAFAFAVYGAFIPAIPVAVVNGFIAVIDVYYLWQMSRRKEYYRTFEIAHTAPFLRHFL
                     QFRERDIQKFFPDFSWEKEMKKAPSCFFLLRDTNPTGVFVYQIKKKAVIVKLDYVIPE
                     YRDMKNARYLFMVLNERFRSEGLREYVVEGVHVETHRKYLTAIGFEADERDPAIMRMP
                     I"
     gene            complement(1749..3578)
                     /locus_tag="PLD82_00860"
     CDS             complement(1749..3578)
                     /locus_tag="PLD82_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG63969.1"
                     /translation="MKKMVLFRVNAGNEYGSGHLERCLVLARLLRKQYDPYFLVKGDN
                     RIGEILYKENLNFKLAMHGFREEVDDIRGLRPNLVFLDVMETPIEFVRAIKEYAPVIT
                     LDDRGSGSEAATVTVYSLPLEKPKMSNIDSNKYIVFRPEIADYEKTRYERKIRRILVT
                     FGGSDPANLSNVFVRISKLSDPKIEWVFVRGQFNQNRWSEGNYNELKHGINLFEQIVQ
                     ADLVVTSFGMTAYESAAIGTPVILCNPGQYHENLSEVAGIFGRLGVYKPAGKDGSDNT
                     SVLAREFSARIGDADQLMQQALQAREQVDKQGAGRMIELIESVLDTGRQEACVVCGSR
                     RERALARDLEKNIFVCEKCGLFWQHWFTLPLFVYEKPYFGEDYAAQYGKTYLEDRENI
                     DRLGKARLEVINGIQRKLEKKYPVTEKNLLDIGCAYGFFLDLARDLGGWTVKGIETSK
                     IASKYARENLVLDVESGGFLDIDVPKEFYQAISLWYVIEHLPKLNDVVEKLYTSLARG
                     GILALSTPNNAGFQGRYRRSEYLSQHPADHYFDFSIRTMKVLLKRYRFKVVKVRVTGI
                     HYERFLKGREGSFWDNAFFRRCYGFLARVFGLGDTFEIYAVKK"
     gene            complement(3710..4393)
                     /locus_tag="PLD82_00865"
     CDS             complement(3710..4393)
                     /locus_tag="PLD82_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOG63970.1"
                     /translation="MNVAGIIQARTGSTRLPGKVCMTLAGVPFIQRVIRAVKAATMVQ
                     EVILAVPESDYGVLEPCARAEDCRIIGGPEDDVLERYLMAARLAGCDTIVRATGDNPF
                     VCPEHIDTILRFHFSQDADLSHWLGIPIGTGVEVIRRDVLETAGREALLPEEREHVTP
                     WIYAHRELYRVEEPVLLKDPGVRLTVDTAEDYARASRLAEYFHQRNMLPVRLDQVLEV
                     YRADPGIFR"
     gene            complement(4390..5526)
                     /locus_tag="PLD82_00870"
     CDS             complement(4390..5526)
                     /locus_tag="PLD82_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63971.1"
                     /translation="MMRETGFTQLGKRFSAQEKRDYARRIELSVLCINVSGQTFRMPA
                     FERTAAMVAGLAPLAELILVDLDAAHSRNDLLLAEYPWMRVIIPTREIGLPAALFLGM
                     REALGQRVLVLDTATAIAYFDIPGALRAFREERRLFAIGPSIIAEDETECPVVLGGCI
                     EDGRLQMTEAVASETRYSLALRRFYGLYDREKVLFLGTPPTSLPLDWAVLEWFISAWS
                     RGWRTVINPEHCFYIPGSWPRSVLPGKAFVDRVRFYRYEIGFLRRHCRSREQVRARRR
                     SIFRRALTNLLKLDFSLALAGLTHNRLRRLSRNTLCSLQDVFMMASADIRQSVLPEGQ
                     VSEAPGENGQAEEAIHEFGQAAGLADRIAASEQPAEEHPGEEHP"
     gene            complement(5532..7049)
                     /locus_tag="PLD82_00875"
     CDS             complement(5532..7049)
                     /locus_tag="PLD82_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HOG63972.1"
                     /translation="MKPVLLVINALDVRDEATALFAGRPVWQETLRRIASLSEGCDLL
                     LVRHEGESLLPEACDVPQTRLSGYGAHAVLSTLQHNHSGYEWYVFLDISAVFMDPGLV
                     TAFLGTGRHRIAQYVYGEHYPKGIAPQVLSAEALAILAGVSSGNDFRFGEEAIFDLMG
                     LDINSWDIEMVVSETDFRRMRLDLRIRSAEALARMKALLAIDPGLVDDWSYERLARCL
                     REHVGILRTVPAYVELDLVDACQLSCHYCPRSVREDLVPEQSVSLDDLGRLVKDLRRF
                     APDATIALSPYSEPLLHPQAETAIRMILDSGLRLVLETNGIALDEKLAALLASADPER
                     SIIIFSPDFSDPERFAREKGASLLPLVEANIERLLALRQRNVWIQAIQFEADDANLDA
                     FYERWKAHEDAILPRKYNNWCQCIPGRPAVDLSPLVRTPCWHLARDLVIRADGRVPMC
                     KQDLECRVSPGNVFRDGLEAVWAGLERQWQEQCADPAFQGLPLCAPCDEWHTYNF"
     gene            complement(7046..8014)
                     /locus_tag="PLD82_00880"
     CDS             complement(7046..8014)
                     /locus_tag="PLD82_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG63973.1"
                     /translation="MAKSACLELVEFIPASFIMSCLRLVPLRWATRSGAAFGRLVWRL
                     APSQRRTAYSGLDLAYGDGMPVSEKKRIAKASFESMGKTIFEFMQFPKLSREAILARV
                     RFVNLEAMDRALAQGRGVIAATAHLSNWEFFAAAFAATGRPLAAIVRPLDNRRLDRHV
                     ENLRSSKGVEVVPRGVALRLGLTALKKGKVLAFLMDQNAARHGVFVPFFGKPAATVSG
                     PAAIALRLDVPIIFCYSRREDDGLFSLIFESVEPVRDRGDEERNIIATTALLTACIER
                     AIRDRPEQWLWMHPRWRTGPEDACDAVSRRSAELGGWIQIRREENE"
     gene            complement(8021..8779)
                     /locus_tag="PLD82_00885"
     CDS             complement(8021..8779)
                     /locus_tag="PLD82_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63974.1"
                     /translation="MDNNEQGKKGLSSCMRTIIIGAGALVLGFGIGFVISGGCSGLSP
                     MGMVRSLESRAASLTPGEDGAILATIEDETITRKTFDQQLSLFLTSNQRMSQEEIVKM
                     KNNPRFLKQFLRQLVDNRILLKAMEKDPDFMNNPELLVFMNLSLSDALQKYYLYKKAG
                     NISVDTNVSDADVEKAYNQLKADPRYAERLDRVPFDQLKTMLSQEIVKQRQMAEVQDL
                     LNKVKAGFRHSVHDEIFGKTPHGTNDAPVMPGMK"
     gene            complement(8836..>11317)
                     /gene="mfd"
                     /locus_tag="PLD82_00890"
     CDS             complement(8836..>11317)
                     /gene="mfd"
                     /locus_tag="PLD82_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG63975.1"
                     /translation="DELYNGYYSPMKLKLPPAALYQPIEEKQGFDLRFETLATSEVST
                     VPMPFSAPRQFMGDLQALSGAIHERLAKEQEVHVFATYEGQAERVASLLSDCAPVRSM
                     AEAGPGRLHVGHAPLSAGFVSDELGLAVILEREIFNRKRSLRRSVRSVNSVPIDSFLD
                     LKPGDPVVHIQHGIGLFVAIERMNSLGLDKDYLKLEYRDGEKLYVPLEMINQVQRYIG
                     QDGAALKLDSLGGRSWERTKEKVKKNVEKLAKELVEIYAARMTLEGYSFGEDTRWQHE
                     FESAFEFEETPDQIKAIEDARADMQSLRPMDRLVCGDVGFGKTEVAMRAAFRAAMAGK
                     QTAILVPTTILAEQHYATFRERFSLFPAVHIDYLNRFRSAAEQKEVISRLAAGHIDII
                     IGTHRLVSDDVRFKNLGFVVIDEEQRFGVQHKEKLKKMRRMVDVMTLTATPIPRTLHM
                     SLIKVRDMSVIRTPPRSRLPVETYVLEFNENMVKRAIMAELDRGGQIYYLHNRVQSIE
                     NVRQFLLKLVPHARIGVAHGQLHEHQLEKVMRDFIDGHLDILLCTSIIESGLDIPNAN
                     TLIVDRADAFGLGQLYQIRGRVGRSTRRGFAWLFYPPDRSLTEQAQKRLQVINEFTDL
                     GSGFDLAMRDLEIRGVGNLFGAEQSGDIISVGFDMYCRILDDAVRELGADFHEEQPDT
                     LLNLDFEGYIPDEYITDERQKIEIYKRIAACTSEADVDALAQEVRDRFGPIPEIVSSL
                     FRLSGLKAEGRAAGVRSLVQQGRYIAIEFAKNHDIDPARVVGLVQTAGARIVPGEKLL
                     LLVPDPGGELADKVDFLKKTLQDLRQYS"
BASE COUNT         2628 a         3383 c         2979 g         2327 t
ORIGIN      
        1 gacaccgaca aagacgtcgg caacacgcaa ggcatccgcc aggctcccac cccgggaaac
       61 cgcaaaggcc tgtttgtacc cgttcagatc gttccgatca atggagacaa tccctttgga
      121 atcgcagaga atgatttcgc cgacgccccg accacgcaag attccggcaa cggctatccc
      181 ggccgcccct gcgccactga cgacgatctt gagggactcc atcttccggc ccgtgatgca
      241 cacagcattc tcgagcgccg ccaatgcgac aatggccgtt ccgtgctggt catcatggaa
      301 aaccggaata tcaagcatgc catccagccg tttttcgatc tcgaaacacc tgggagcact
      361 gatatcctcg aggttgattc cgccgaagac cagtgcgatg cgcgccacag tctcgacgat
      421 ttcatccgga tcccgggtat ccagacagac cggaaacgca ttgacgcctc caaagacttt
      481 gaacagggcg gccttgcctt ccatgacggg caaggctgcc agtgtcccga tgtcgccaag
      541 tccgagaacg gctgtcccgt ccgtgatgac agcaacggtc gttcccttga ttgtcagatc
      601 ccaggccgcc tcggcatcgc tctcgatatg cttgcagacc cgcgccactc ccggagtgta
      661 ggccatcgcc aactcatccg gaccagagag ggctgtcctg ctctcgatcc cgagcttccc
      721 cccatggtgc agttcaaaag tcctgtcccc attctccacc aggttggatg cctggagtgt
      781 tacaagcctt ttaccaagtt catccgccgc tgactgcccg ggaaggaaaa tgacagcctc
      841 ggcaagcgcc ggatcattcg ccgaaccagc cgggcaaaac gaggtcagca ccccgtcaac
      901 cgaagctgtc agttccgcca tctcgggcag cgcccgggag gcatctcgta cacgtatcag
      961 aacgcgccta ctgtatgcgg ctgtcgctgt ttgtcttcct gccatagtgt tcttgcttcc
     1021 cccgcctgga cccgctcaga tcggcatgcg catgattgcc ggatcacgct cgtctgcttc
     1081 aaaaccgatt gccgtcagat atttgcgatg cgtctcgaca tggacaccct cgacaacata
     1141 ctccctcagc ccctcgctcc tgaagcgttc attgaggacc atgaagaggt agcgggcatt
     1201 cttcatgtcg cgatattcag gaatcacata atccagcttg acgatgaccg ccttcttctt
     1261 aatctgatag acgaaaacgc cggtcggatt ggtatccctc agaaggaaaa agcaggatgg
     1321 cgccttcttc atctcctttt cccaggaaaa atcgggaaaa aatttctgga tgtctcgttc
     1381 ccggaattgc aggaaatgcc gcaaaaaagg cgcggtatgg gctatctcga acgttctgta
     1441 gtattccttg cgccgggaca tctgccagag ataatacaca tcaatcacgg cgatgaaacc
     1501 gttgacgacc gcgaccggaa tggccgggat gaatgcacca tagaccgcaa aggcaaaagc
     1561 gccgatgaaa ttgataactc ggagcttgta gatattgctc atcatcaggg agacagcaat
     1621 caacacggat gagacatatc cgacaatctc caagagtgtc actcctccgt ccggcggtgg
     1681 tggcattgga atcatctttt cctcctgtaa caactgatag acaattcaga cccatggcct
     1741 gaggaatgct actttttgac agcatagatc tcgaaggtat cacccagtcc gaaaacacgg
     1801 gccaggaatc cgtaacagcg ccggaagaag gcgttgtccc agaagctccc ctctcgcccc
     1861 ttgagaaaac gctcataatg gattcccgtc acccggacct tgacaacctt gaagcgatag
     1921 cgtttgagaa ggaccttcat cgtacggatg gagaaatcaa aataatgatc cgccgggtgc
     1981 tgcgagagat actcgcttct tcgataccgc ccctggaatc cggcattatt gggagtcgag
     2041 agtgccaaaa taccgcctcg cgccaaggac gtatagagtt tttcaaccac gtcattgagt
     2101 ttggggagat gctcgatcac gtaccagaga ctgatggcct gatagaactc cttgggaacg
     2161 tcaatatcga gaaagccacc cgattccaca tccaggacaa gattctcccg cgcatatttt
     2221 gaggcgattt tcgaggtttc gatgcccttg acagtccagc ccccaagatc gcgggccaga
     2281 tcgaggaaaa acccgtacgc acatccgata tccagaagat tcttttcggt tacgggatac
     2341 ttcttctcga gcttgcgctg aatcccgttg atgacctcga gccgagcctt ccccaggcga
     2401 tcgatgttct cccgatcctc gagataggtt ttcccatact gtgccgcgta atcctcgcca
     2461 aagtagggtt tctcgtagac aaaaagcggc agggtgaacc agtgttgcca gaaaagtccg
     2521 catttttcac agacaaaaat gtttttttca agatcacggg cgagggcacg ctcccgccga
     2581 ctcccgcaga cgacacaggc ttcctgccgg cccgtatcaa gaaccgactc gatcagctcg
     2641 atcatccggc cggcaccttg tttgtccacc tgctcccgcg cctgcagcgc ctgctgcatc
     2701 aactggtcgg catccccgat cctggccgaa aattccctgg cgagaaccga ggtattgtca
     2761 cttccatcct tgccggccgg tttatagaca cccagccgac cgaatatccc ggcgacctcg
     2821 gagagattct cgtgatactg tcccgggttg cagaggatga caggtgtccc gatcgccgca
     2881 gattcgtagg cggtcatccc gaaggaagtc acaacgagat ccgcctgcac gatttgttca
     2941 aacaagttga taccatgttt caactcgttg taattcccct cggaccagcg attctggttg
     3001 aactgcccgc ggacaaaaac ccactcgatc ttcgggtcgg acagcttgct gatgcgcaca
     3061 aaaacattcg agagatttgc aggatcggaa ccgccgaatg tcacgagaat ccgccggatc
     3121 ttgcgttcat agcgggtctt ctcgtaatcg gcgatctccg gccggaaaac aatgtatttg
     3181 ttggaatcaa tatttgacat cttcggcttt tcgagcggaa gggagtacac cgtcaccgtt
     3241 gccgcctcgg agcccgatcc cctgtcgtca agggtgatca ccggcgcgta ctccttgatc
     3301 gcacggacaa attcgatggg ggtttccatg acatccagga aaacaagatt gggccgcaac
     3361 ccgcggatat catcgacctc ctcacggaat ccatgcatag ccaatttgaa attaagattt
     3421 tctttataca gaatctcgcc gatgcggttg tcgcccttga cgaggaaata cggatcatac
     3481 tgtttccgca gaagccgtgc cagaaccaga caccgttcga gatggccgga accgtactcg
     3541 ttcccggcat tgacacgaaa aagaaccatt tttttcatgc aaaatcgctc ccgcaccgta
     3601 tttcccgccg aagtttaccg gcaaaggtac agtatgaaag ctaacgattt ttttcattcg
     3661 tgatgatgct ttcgggtgaa caccctcagg ccattccagg atacggctct taccggaaga
     3721 ttccgggatc ggctcggtaa acctcaagaa cctgatcgag ccggaccggg agcatattcc
     3781 tctgatggaa atactctgcc aatcgggaag cccgcgcata atcctcggcg gtatccacag
     3841 tcaaccggac cccgggatcc ttcaagagaa ccggctcctc cacccggtac agctcccggt
     3901 gggcatagat ccagggagtg acatgctccc gctcctcggg aagcagggcc tcacgcccgg
     3961 ccgtctcgag gacatcgcgc cgaatcacct cgacaccagt cccgatcggg atgcccaacc
     4021 agtgggagag gtcggcatcc tgcgaaaaat gaaaccggag gatggtatca atatgctccg
     4081 gacagacaaa gggattgtcg ccggtagccc ggacgatggt gtcgcacccg gcaagccggg
     4141 ccgccatcag ataccgctcc agaacgtcat cctccggacc tccgatgatc cggcagtctt
     4201 ctgcccgggc acagggctca agtacaccat agtcactctc gggaaccgcc aggatgacct
     4261 cctgcaccat ggtggccgcc ttgaccgccc tgatgacacg ctgaatgaac ggaacaccgg
     4321 ccagcgtcat gcacaccttg ccgggaagcc tggtcgaacc ggtcctcgcc tggatgatac
     4381 cggccacgtt cacggatgct cctcccccgg atgctcttcg gctggttgtt cgctggccgc
     4441 aatccggtct gcgagtccgg cagcctgccc gaattcatgg attgcctcct ccgcctgccc
     4501 attttctccg ggtgcttcac tcacctgtcc ctccggaaga acggactgcc tgatatcggc
     4561 ggaggccatc atgaagacat cctgcagact gcagagggtg ttccggctca agcgtctcag
     4621 cctgttatgc gtcaaaccgg ccagcgccag actgaaatcc aatttgagga gattggtcaa
     4681 ggcacgccgg aaaatactgc gtctcctggc ccgaacctgc tcccgggagc ggcaatgccg
     4741 gcgcagaaat cctatctcgt accggtaaaa ccgtacccgg tcaacaaaag ctttccccgg
     4801 gagcacagaa cggggccagg atccgggaat atagaaacaa tgttccggat tgatcaccgt
     4861 tcgccagccc ctgctccagg ccgatatgaa ccactcaagg acggcccaat ccaggggaag
     4921 agacgtcggg ggtgtgccaa gaaaaagcac tttttcccga tcatagagcc cataaaaacg
     4981 ccggagcgca agggaatacc gggtttccga cgcaactgcc tcggtcatct gcaatcgccc
     5041 gtcctcgatg catccgccca ggacaaccgg gcattccgtc tcgtcttcgg caatgatgga
     5101 cggaccgatc gcgaacagtc gacgttcctc ccggaacgca cgaagcgcac cgggaatgtc
     5161 aaaatatgcg atcgccgtcg cggtatccag aacgagaacg cgctggccaa gggcttcacg
     5221 cattccgagg aacagtgccg caggcaagcc aatctcccgg gtcgggatga tgacccgcat
     5281 ccagggatat tcggccagaa gcagatcatt gcgactgtgg gcagcatcca gatcgacgag
     5341 aatcaactcg gccaggggag ccaggccagc aaccatggcg gccgtccgct caaacgcagg
     5401 catgcggaat gtctgcccgg aaacattgat gcacaaaaca ctgagctcga tccgccgggc
     5461 gtagtcccgc ttttcctgag ccgaaaaccg tttcccgagc tgggtaaacc ccgtctctct
     5521 catcatccat cctaaaaatt atatgtgtgc cattcgtcgc atggcgcaca gagtggcagt
     5581 ccttgaaacg ccggatcggc acattgttcc tgccattgtc tttcaagtcc ggcccaaacg
     5641 gcttccaatc catcacgaaa aacgttcccc ggagaaaccc ggcactccag atcctgcttg
     5701 cacatcggaa cccggccatc cgccctgatc acaagatcgc gggccagatg ccagcaaggg
     5761 gtccggacga ggggtgaaag gtccactgcc ggtcgacccg ggatacattg gcaccagtta
     5821 ttgtacttcc tcggaagaat ggcatcctcg tgcgccttcc agcgttcata aaacgcatcc
     5881 aggttcgcgt cgtccgcttc aaactggatc gcctggatcc agacattgcg ctgcctgagc
     5941 gcaagcaaac gctcgatgtt ggcttcaacg agcggcagaa ggctggcccc cttctcgcgg
     6001 gcaaagcgct ccgggtcgga aaaatccggc gagaaaataa tgatggaacg ctcggggtcc
     6061 gcactcgcca ggagcgcagc cagtttctcg tcaagcgcta tcccgttcgt ctcaaggaca
     6121 agacgcagac cggaatccag gatcatcctg atcgcggttt cagcctgggg gtggagcaag
     6181 ggttccgaat acggtgaaag tgcaatggtg gcgtccggag cgaatcggcg gagatccttc
     6241 acaagtcggc cgagatcatc cagcgacaca ctctgttcgg gaaccaggtc ctcgcgaacc
     6301 gagcgcgggc agtagtgaca ggagagctgg caagcatcaa caagatccag ctcgacatag
     6361 gcgggaaccg tcctgaggat gccgacatgc tcccggagac atcgagcaag cctttcgtac
     6421 gaccagtcat cgacaagccc tggatcgatg gccaggagtg ccttcattct ggcgagggcc
     6481 tcggccgatc ggatacggag atccagacgc atccgccgga aatcggtctc cgaaaccacc
     6541 atctcgatat cccaggaatt gatatccaac cccatgagat caaagatggc ctcctcgcca
     6601 aaccggaaat cattcccgct ggagaccccg gccagaatgg caagggcttc cgctgacaga
     6661 acctgcgggg caatcccttt cggataatgc tccccgtaca catactgcgc tatccgatgc
     6721 cgacctgtcc cgaggaatgc cgtcaccaaa ccggggtcca tgaacactgc ggaaatatcc
     6781 agaaaaacat accattcgta cccggaatga ttgtgttgaa gggtcgagag aactgcatgt
     6841 gcaccatagc cagacagtct ggtctgcgga acatcacaag cctctggaag aagagactct
     6901 ccctcatgac ggaccaggag gagatcacac ccctccgaaa gactagctat ccgcctgagg
     6961 gtctcctgcc agaccggtcg cccggcaaaa agagcggtcg cttcgtcacg aacgtcaaga
     7021 gcattgatca ccaggagtac cggtttcact cgttttcctc cctccgaatt tgaatccaac
     7081 cgccgagttc ggcactccgt cggctgaccg catcgcaggc atcctcgggg ccggtccgcc
     7141 atctgggatg catccagagc cattgttccg gccggtcacg aatcgctcgc tcgatacatg
     7201 cagtcaacag agctgtcgtc gcgatgatgt tgcgctcctc gtcgccgcga tcccggaccg
     7261 gttcgacact ttcaaaaatc agggaaaaca ggccatcgtc ctccctgcgg gaataacaga
     7321 agatgatggg aacatcaagg cgcaaggcga tcgcagccgg tccgctgacc gtcgcagccg
     7381 gtttcccgaa gaacgggaca aaaacaccat ggcgggcagc attttgatcc atcaggaacg
     7441 ccaagacttt cccttttttg agggctgtca acccgagtcg cagggctacg ccacggggaa
     7501 caacctcgac gcccttggat gaacgcagat tctccacatg tctgtccagt ctgcgattgt
     7561 ccaacggtcg aacgatggcc gccagcggac ggcctgtggc ggcaaacgcg gcggcaaaaa
     7621 actcccagtt tgaaagatgg gccgtggccg cgatcacacc ccgtccttgg gcgagggcgc
     7681 gatccatagc ctcaagattg acaaagcgga cccgggcaag aatggcctcg cgggaaagtt
     7741 tggggaactg catgaactca aaaatcgtct ttcccatgct ttcgaaggaa gccttggcga
     7801 ttctcttttt ttcggaaacc ggcatcccgt caccatacgc cagatcaaga ccggaatacg
     7861 ctgtcctccg ctgggaaggc gcgagtctcc agacgagtcg accaaaagcc gccccggacc
     7921 tggtcgccca gcgcaagggg accagtcgca ggcaggacat gataaagctc gccgggataa
     7981 actcgacgag ctcaagacag gccgactttg ccatgccggc ttatttcatc ccgggcatga
     8041 ctggtgcatc attggttcca tgcggagttt tcccgaaaat ctcgtcgtga acactgtgcc
     8101 tgaaaccggc cttgaccttg ttcaaaagat cctgaacttc agccatctgc cgttgtttga
     8161 cgatctcctg cgaaagcatg gttttgagct ggtcaaacgg gacacgatca agacgctcgg
     8221 catagcgggg atccgctttg agctggttat aggctttttc gacatcggca tcactcacat
     8281 tggtatcaac actgatgttc ccggcctttt tgtaaagata gtatttctga agagcatccg
     8341 atagcgaaag gttcatgaac accaagagct cggggttgtt catgaaatcc ggatccttct
     8401 ccatagcctt aagcaggatc ctgttgtcaa caagctgtcg aaggaactgc ttgagaaacc
     8461 ggggattgtt tttcatcttg acgatttctt cctgggacat ccgctggttg ctcgtgagaa
     8521 aaagggagag ctgctgatca aacgtttttc gcgtaatcgt ttcgtcttca atcgttgcga
     8581 gaatggcgcc atcctcaccg ggagtcaagc tggcggcccg ggactcaagg ctacggacca
     8641 tgcccatcgg ggagagcccg gaacatccgc cactgatcac aaacccgatg ccaaaaccaa
     8701 ggacaagcgc cccggctcca atgatgatcg tccgcataca ggatgacaga cctttttttc
     8761 cctgctcatt attgtccata ctgtgttgaa aatcctttcg tgaaggtatc aagtcaggcg
     8821 gtgttccacc agcactcatg aatactgcct caaatcttgc aaggttttct tcaaaaagtc
     8881 aaccttgtcg gcaagctcgc cgccgggatc gggaaccaac agcaggagtt tttcgccggg
     8941 aacaatcctc gcacctgctg tctgcaccaa tcccacaacc cgcgccgggt caatgtcgtg
     9001 gtttttggca aactcgatgg cgatataccg cccctgctgg accagactgc gcacaccggc
     9061 cgcccgcccc tccgccttga gtccggaaag cctgaagaga gaggatacga tctcgggaat
     9121 cggtccaaac cggtcacgga cctcctgtgc cagggcatcc acatccgcct cgctcgtgca
     9181 tgcggcaatt ctcttgtaga tttcgatttt ctgtcgttcg tcggtgatgt attcatccgg
     9241 aatatatccc tcaaaatcca gattgagcag ggtatcaggt tgttcctcat gaaaatccgc
     9301 cccgagctca cgcaccgcat catcgaggat cctgcagtac atatcgaagc cgaccgaaat
     9361 gatgtctcca ctttgctcgg ccccaaaaag attgcccaca ccccggatct caagatcccg
     9421 catggccagg tcgaaaccgg aaccgagatc ggtaaactcg ttgatcacct ggagccgttt
     9481 ttgggcctgt tccgtcagcg agcgatcagg aggatagaag agccaggcaa acccccttct
     9541 tgttgaacgc ccgacacggc cacggatctg atagagctgc ccaagaccaa aagcatcggc
     9601 acgatcgaca atcagggtat tcgcattggg aatatccagc ccggactcga tgatcgatgt
     9661 gcagagcagg atatccaggt gcccgtcgat aaaatcgcgc atcacttttt ccaactggtg
     9721 ctcgtgcagc tggccatggg ccacgccgat tctggcatgg gggacaagct tgagaaggaa
     9781 ttggcggaca ttctcgatgg actggacccg gttgtgcaga tagtagatct gcccgcccct
     9841 gtcgagctcg gccatgatgg cacgtttgac catgttctcg ttgaactcga ggacataggt
     9901 ctcaaccggg agtctcgagc ggggtggagt cctgatgaca ctcatgtccc gcaccttgat
     9961 gagagacata tgcagggtcc gcggaatcgg tgtggccgtc agtgtcatca catcaaccat
    10021 ccggcgcatc tttttcaatt tctccttgtg ttggaccccg aagcgttgct cctcgtcgat
    10081 cacgacaaaa ccgagattct tgaaacggac atcatccgaa acaagccgat gcgtcccgat
    10141 gatgatatcg atgtgtccgg ccgccaggcg gctgatgacc tctttctgtt cggctgcact
    10201 ccggaaccgg ttgagataat cgatgtgcac tgccggaaaa agggaaaacc gctccctgaa
    10261 tgtggcgtaa tgctgttcgg cgagaatcgt cgtcggcaca agaatggccg tctgctttcc
    10321 ggccatggcg gcccggaacg cggctcgcat ggcgacctcg gtttttccga aaccgacatc
    10381 accacagaca agtctgtcca tgggacggag gctttgcatg tcggccctgg catcctcaat
    10441 cgccttgatc tggtctggcg tctcctcgaa ttcgaaggca ctctcgaatt catgttgcca
    10501 acgggtatcc tctccgaagg aatatccttc aagagtcatc cgggcggcat agatctcgac
    10561 aagctccttg gccagtttct cgacgttctt tttgaccttt tccttggtac gctcccagga
    10621 gcgccccccc agggaatcca gtttgagggc cgcaccatcc tgcccgatat agcgctggac
    10681 ctgattgatc atctcgagcg ggacatagag cttctctcca tcccgatact ccagcttgag
    10741 ataatccttg tcgagaccga gggagttcat ccgttcgatc gcgacaaaga gcccgatccc
    10801 gtgctggatg tgcacaaccg gatcacccgg cttgagatcc aggaacgaat cgatagggac
    10861 agaattcacg gaacggacgg agcggcggag agaacgtttg cgattgaata tctcgcgctc
    10921 gaggatgacc gcaaggccga gttcatccga gacaaatccg gcggaaagag gcgcatgccc
    10981 gacatgcagc cttcccggtc ccgcctcggc cattgaccgg acgggggcac aatccgaaag
    11041 gaggctcgcg actctttcgg cctgtccttc gtaggtggca aaaacatgaa cctcctgctc
    11101 tttggccagc cgttcatgaa tggcgccgga gagcgcctgg agatccccca taaattgtct
    11161 gggggcactg aacggcatcg gaactgtcga gacttcactc gtcgccagag tctcgaatct
    11221 cagatcgaag ccctgcttct cctcgatcgg ttggtacaag gccgcaggag ggagcttgag
    11281 tttcatcggg ctgtagtacc cgttatagag ttcatcg
//
LOCUS       DAOSHE010000014        11093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_4956_length_11093_cov_4.192970, whole genome shotgun sequence.
ACCESSION   DAOSHE010000014 DAOSHE010000000
VERSION     DAOSHE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4956_length_11093_cov_4.192970"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLD82_00895"
     CDS             complement(<1..1053)
                     /locus_tag="PLD82_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG63976.1"
                     /translation="MHRQHTFKPWVIGFSYLAVTIGANLLAVIGNTILMSYGLFLSPE
                     EHVQFMRAVDLSLDYEAAMQILSFGIPTLLVMLYIRPIHRALRSGLITDPRARRHVLN
                     APLVISICSLVGWIIAIVEIVILHGLILQSEGNLLSIMGKNIVIAFLTGVFCFVLTFY
                     SLELMARRWLVPRFFPDNKLTEVPGVIRLSIRARLFIFWLAIMVFPSAIWITITLSLL
                     PRAGAGDLVPVAINLGLILLFLGSLITHLLAGTWQKPLIEMKQATDNIRAGDYNITVG
                     VRSVDEAGHLGEGINEMAGGLREKEFIKDTLGRIVDPAIRDHLLRGKITLGGEIRDAV
                     ILFSDIRNFTGLSETMR"
     gene            complement(1142..1456)
                     /gene="cutA"
                     /locus_tag="PLD82_00900"
     CDS             complement(1142..1456)
                     /gene="cutA"
                     /locus_tag="PLD82_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOG63977.1"
                     /translation="MRIVISNCRPEEADRIVDTLLSEQLISSCNILPSITTKAHWKGE
                     IQTEQESILIMRTRAELVWQLESRILEINSFEMPEVAAIEVQEWNKKYLKWIYDSTKP
                     TG"
     gene            1617..3533
                     /locus_tag="PLD82_00905"
     CDS             1617..3533
                     /locus_tag="PLD82_00905"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG63978.1"
                     /translation="MMVGGQVNLFDLTLAGLIGISLFQGVHVLRRDPRGSLNRIHAAL
                     CLANVIWTMSLLAVTLLDARTDSVALWGWYRFGALGWSLIPALFLHFSLRLVGHGGWR
                     LVVPAYLAGFVFFLCNLSDPVLVSGFAGGPWGTVLVHDPARLSYIVFVVYNSSSIVAA
                     AIHLLRWGRKGRERKQARIIAWSGLVTLVAVYSANLVLPELAPGLPAPGAILTLITLF
                     GIWYAVRRHRLLAMDPGIAAGEIMMRMMDMLFVLDDDARIVDANPQVVQNLGYRVSEL
                     RKRPVRDLLVEREEFDRELGIIRVQPTLCARFVAHFHASSGIQVPVVLFLSLVADRYK
                     SIAGGVLVAHDLRGTLQLEEEIAERRRAESSLKTLMHELENERNLLRRRNETMEQELD
                     LARRIQMQIVPRAVPGPGIAFAYLPMSKVGGDYFDFLQTGDPDEVGCFISDVSGHGVP
                     AALITSMIKAFLLQAGSLARDPAALMDALNTVLNGQTGGNFVTAWYGIVNYRSRTLVW
                     TNAGHGDPWLVASGTVSKLEGPWHNLPLAVMSGGGLAGIGREYKSARSVLPGYCRIFL
                     CTDGLLESAPHENPEDLFGGSALDGVLQRTAGLDSSACVDAILAELSEFRGGLDYEDD
                     ICVVCLDIPPEGRP"
     gene            complement(3603..4103)
                     /locus_tag="PLD82_00910"
     CDS             complement(3603..4103)
                     /locus_tag="PLD82_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63979.1"
                     /translation="MTRIYSYDEYKEMTFEAVEDFNNREYGKALEKFLDLAETNPENP
                     KVHEILVLIYLKLDMLDKAQAEFETYHKLLGQSVPGLALPPRKTFGQLASEAGDRVEL
                     ERACDAIMSGSEKLDPWQSTDMVSKLGIVYMSQGEYRKAEELLLGFKQKLLENCPAGI
                     RESIAV"
     gene            complement(4198..6390)
                     /locus_tag="PLD82_00915"
     CDS             complement(4198..6390)
                     /locus_tag="PLD82_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG63980.1"
                     /translation="MTQSTPARILIVEDDPVVSATLEHMLLSAGYTIAGKASGGHKAV
                     ELAGQLAPDLILMDILLDDDLDGITASHEIARATATPVVFISSLDDAETIESTRKSGP
                     FGYIRKPCDERELVIVIENALYRHSTEKDRDSHNRRLSMILENMGEALIYCLADGSID
                     YINPAGEALFDCRLSSVFGGPLSQLFSLPGSTQPPQPDTLPEETRIITASGQQLDVTI
                     RHTTLHGDDGMIFLIRNEEERARHEESRRESERQFHQLVELSPEAIFVVDEDEIVFMN
                     AAGKRLLNLPPNGRAPGASRLFEGIAIRDAGSDWSQAVETTLILPSGGSIDTEILATA
                     ITFRGRPCIQLLCQNITARKQRMDSERQAHKMEAIGRLAGGIAHDFNNILTAILGYCE
                     LAQLQIPDEYPGREEIEQIRQAADRASGLTRQLLAFSRRQVLQRKLLDPVLIVSEMEK
                     LLRRVIGENVELIINTDPEVSPILADASQMQQVVINLAVNARDAMPEGGTLTIEIRNS
                     STPKTLSGVELLVSDNGCGMSEDIRRHLFEPFYTTKAPGQGTGLGLSTVYGIITQTGG
                     TISVQSSPGAGTTFRIFFPAADPSLITPQTSPIDTDTQGVHNETVLLIEDDAMVRSLA
                     ATFLRRNGYVVLEADSGSTGLAMAQTQQGRLDLLLTDMILPDQSGEKIAKQIQANHPE
                     TAVLFISGYGDSRSRTGQEKSQPRLLQKPFTGSELLMTVRQILLHPRG"
     gene            complement(6489..7529)
                     /locus_tag="PLD82_00920"
     CDS             complement(6489..7529)
                     /locus_tag="PLD82_00920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG63981.1"
                     /translation="MPAQNHGTTMKNQYPLKALFSQIGMLITSSLELPVILDRIMHEI
                     REYFEPQNWSLLRLDPNTNELFFMIAEGIDTGLMEDLRLRPGEGIAGKVAQTGQAIYV
                     PDVTKSDLFSARVDAISGVTTRSIIAVPLNFRNRVYGVIELINTEEGPLFSDDDYLVL
                     QTIADFAAIAFANAQMYEEAILIANHDPLTGLLNRNRLEEILGQYDSSDHPARRSMDI
                     REQALTIVIDLDNFKEVNDRHGHQAGDRVLRHTAQSLRSCMRAEDLLFRIGGDEFLAL
                     VFIPAHGVEPETVREQFTRKLGICQCQDSREPFTVRLSWGMACGPVTRLRALINEADQ
                     AMYHLKRSRKGI"
     gene            complement(7592..9220)
                     /locus_tag="PLD82_00925"
     CDS             complement(7592..9220)
                     /locus_tag="PLD82_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HOG63982.1"
                     /translation="MIAIILLVVFFAVMIGIGIWGMRKTSSLSDFFLGGRSIGPWVSA
                     MAYGTSYFSAVLFIGFAGKFGWGLGLDVLWIILGNTFFGALAAWLVLGRRTRRMTQNL
                     DVMTMPEYLQERYGAKSFKMVSAVIIFVFLLPYCASVFKGLGHLFEINFGMSYDVALL
                     IMIGITGVYMILGGYFAITLTDFIQGIIMIIGSIAMIAIMVGEAGGVSKAIAAVQSEY
                     AAHVPSGAQPGWLMVFSLVFMTSFGTWGLPQMVQKFYAIKSEGVIIKAAIVTTVFSLV
                     IVFAAYFNGALSHVFYEAPKKSVQTTAQQAPQQKPAAVSTTTAGNETLKRTATTQAAA
                     TAQPSDTQVKTKPVVPNKDGKVDYDRIVPDMLSTHLPELLMAVILLLVLSASMSTLSS
                     LVLVSASAVAIDMYKGHINPNISKENSVAMMRFLSGLFIIISFFIARYELSVIVTLMS
                     LSWGAVAGTFMAPFLYGLYWKGATRTGIAAGMGTGLVLSISLFFILGPNNSPIASTIA
                     MIAPFLVIPLVSIWTRKPDQALLAKAFDGIGRKR"
     gene            9462..9989
                     /locus_tag="PLD82_00930"
     CDS             9462..9989
                     /locus_tag="PLD82_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63983.1"
                     /translation="MKYSTWIVGVCLLAFVVLPGVLAADEEQTVAAVDFKKLVALLAT
                     PPAGFTAVGKAEGSRLDMGEGSWSTAVREFKKGQKRVKITILDGAYMEQAYAAFNALK
                     MFSYETSDGYVKGVTINGHAGIRQQENSSKKHTVMLLVAGRFLVQWEGKKLASMDELD
                     AIVNGFDFSGLTALK"
     gene            complement(10020..>11093)
                     /locus_tag="PLD82_00935"
     CDS             complement(10020..>11093)
                     /locus_tag="PLD82_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG63984.1"
                     /translation="STFTMGNFERGILELPRELPGLLVIFVSALLAFLCPRHLAVTAN
                     ALTGLGMFLIAFFSSSFNTMLLWLFIGSVGQHLFFPLQSSIAMELAPEGHTGRVLGRL
                     QSWGNMAGLAGSLVVWGGFRLAGFDFTTSFVIGGCGFFFAALMIALMSRDQPHPASSR
                     FRLRREYGLFYWLTVLYGTRKQLFLTFAPWVLVTIFKEPTETIAILLFIGGVIGIFFK
                     PWLGHLIDTRGERFVFTVEAILLIVICLGYAFASDLFIPSVALVLTAICYVVDQLLVS
                     AGMARATWLKKIALAPEDVQQTLTMGVSIDHVFSISIALIGGLLWHELGHSAVFLVGA
                     GIAVVNLISARFVRVPGHKKTAP"
BASE COUNT         2330 a         2811 c         3491 g         2461 t
ORIGIN      
        1 ccgcatggtt tccgagaggc ctgtgaaatt cctgatgtcg ctgaagagga tgaccgcatc
       61 cctgatttca ccacccagtg tgatttttcc acggagaagg tgatcccgta tggctggatc
      121 gacgattcgc cccagggtat ccttgatgaa ttctttttcc ctcaggcctc ctgccatctc
      181 gttgattcct tcgccgagat gaccggcttc gtcaacagag cggacaccga ccgtgatgtt
      241 gtagtccccc gcccggatat tgtccgtggc ctgcttcatt tcgatcagcg gtttttgcca
      301 ggtgccggcc aaaagatggg tgatcagtga tccgagaaag aggaggatca ggcccaggtt
      361 gatggcaacg ggaaccagat cgccggctcc ggcacgggga agcagggaga gcgtgatcgt
      421 tatccatatg gcggagggaa acaccatgat ggccagccag aagataaaca ggcgggccct
      481 gattgacagc ctgatgacgc ctgggacctc ggtcagcttg ttgtccggaa aaaatcgggg
      541 tacaagccag cgacgggcca tcagttcaag cgagtaaaag gtgagcacaa agcagaagac
      601 acccgttaaa aatgcgatga caatattttt tcccatgatc gaaagaaggt tgccctcgga
      661 ttgcaggatc agtccgtgca ggatgacgat ttcaacaatg gcaatgatcc agccaacgag
      721 actgcagatg gagatcacca agggcgcgtt cagaacatgc cgtctggcgc ggggatctgt
      781 gatcaaaccg ctgcgcagag cccggtgaat cgggcggata tagagcatga ccagcagggt
      841 cgggatgccg aatgagagga tttgcatggc ggcttcgtaa tccaggctga gatcgaccgc
      901 cctcatgaac tggacatgtt cttcaggaga gagaaagagg ccataggaca tgaggatcgt
      961 attgccgatg acggccagca gattggcccc gatagtcaca gccaggtagg aaaagccgat
     1021 gacccaaggt ttgaaagtgt gttgcctatg catatggagt ccttttcgca tgatcaggtc
     1081 tagaaacgca aacagcagcg caaagcgctg ccgtcggatc gagcaaaggg aaacagagcg
     1141 gtcagcctgt gggtttggtc gagtcgtaga tccacttgag atatttttta ttccattcct
     1201 ggacttcaat ggccgcaact tccggcatct cgaaggagtt gatttcgagg atgcggcttt
     1261 caagctgcca gacaagctca gccctggttc tcatgatcag gatggattcc tgctcggtct
     1321 gaatctcgcc cttccagtgt gctttggtgg tgatactggg aaggatgtta cagcttgata
     1381 tgagctgttc ggaaagcaag gtgtcaacga tgcgatcagc ctcttccggt ctgcagtttg
     1441 agatgacaat acgcatacgg ctcgtccttt gagtgtattt ctggatgctc tgtctgtgat
     1501 tgtatggggg tttatctccg ctgtcaagag gcaatccgga ctctttggaa acctttttgg
     1561 aacgttgagc aggatggaat ctccagcccg cttgtggtaa agtggaacga atatacatga
     1621 tggttggtgg tcaggtgaat ctgttcgatt tgacacttgc aggactgatc gggatcagcc
     1681 tttttcaggg tgtccatgtt ttacgccgtg atccccgggg ctctctgaac cggattcatg
     1741 ctgcgctctg cctggccaac gtcatctgga cgatgagtct cctggctgtg accctccttg
     1801 atgcaagaac ggacagtgtc gcactctggg gctggtatcg tttcggcgcc ctcgggtgga
     1861 gtttgattcc ggcgcttttc ctgcatttct cgctccgcct ggttggccac gggggatggc
     1921 ggcttgtcgt gccggcctat ctcgccggat ttgtgttttt tttgtgcaat ctgtccgatc
     1981 ctgtccttgt ttccgggttt gccggaggac catggggaac agttcttgtc catgatcctg
     2041 ccaggttgtc ctacatcgtt tttgttgtct ataattcctc gtcgattgtt gcggcagcaa
     2101 tccacttgct ccggtggggc cgcaaaggtc gggagagaaa gcaggcccgg atcattgcct
     2161 ggtcgggatt ggtgaccctt gtcgctgtct attcagccaa tcttgtgctg cccgaactgg
     2221 cgcctgggct cccggctccc ggggcaatcc tgaccttgat cacgctcttc gggatctggt
     2281 atgccgtccg gcggcaccgg ttgctggcaa tggatccggg gattgccgca ggcgagatca
     2341 tgatgcgcat gatggacatg ctctttgttc ttgatgacga cgcacggatt gtcgatgcca
     2401 acccccaggt tgtacaaaat ctcggatatc gggtttcgga gttgcggaag cgtcccgtca
     2461 gggatctgct cgtcgagagg gaagagtttg acagggagct cggaatcatc cgcgttcagc
     2521 ccacgctttg tgcgcggttt gtggcgcatt tccatgcgtc ctcggggata caggttcctg
     2581 tagtcctgtt tttgtccctc gttgccgatc gctacaagtc gatcgcgggc ggtgtgctgg
     2641 tggcgcacga cttgcggggg acgctccagc ttgaagagga gatcgctgaa cgacgcaggg
     2701 ccgagtccag tctcaagacc ctcatgcacg aactggaaaa cgaacgcaat ctcctcaggc
     2761 ggcggaatga gaccatggag caggaactcg atctggcacg cagaatccag atgcagattg
     2821 ttccccgggc cgtcccggga cccggaatcg ccttcgccta tcttccgatg agcaaggttg
     2881 gcggggatta tttcgatttc cttcagaccg gcgacccgga tgaggtgggc tgcttcatca
     2941 gtgatgtttc tgggcacgga gtcccggcgg cgctgattac cagcatgatc aaggcgtttc
     3001 ttcttcaggc gggcagtctg gcccgggatc cggcagccct gatggatgca ctgaataccg
     3061 ttttgaacgg tcaaaccggg gggaatttcg tgacggcctg gtacggaatt gtgaactacc
     3121 ggtctcgaac cctggtctgg acgaatgccg gtcatggtga cccctggctt gtcgcgtccg
     3181 gaaccgtatc aaagctggag gggccctggc ataatctccc tctggcggtc atgtccgggg
     3241 gtggcctcgc cggaatcggc agggagtaca aaagtgcacg atcagtattg cccgggtatt
     3301 gccgcatctt tctctgtacc gatgggcttc ttgaatcggc gccacatgag aacccggagg
     3361 atctctttgg tggatcagcc ctggacggtg tcctccagag gacagccggt ctcgattcct
     3421 cagcctgtgt ggatgccatt cttgccgagc tgagtgaatt caggggtgga ctggattacg
     3481 aggacgatat ctgtgtggtc tgtctggaca tcccgcccga gggcaggccc tgagcccgga
     3541 tgctgaacac cccggcgagt tgccggggtg tgatcaagac tgacccgtgt tgcgggacgt
     3601 actcagacgg cgatgctctc gcggatcccg gccggacagt tttcgagtag cttctgcttg
     3661 aatcccagaa ggagttcttc ggctttgcgg tactcgccct gggacatgta cacgataccc
     3721 agtttcgaga ccatgtctgt actctgccaa gggtcgagtt tttcgctccc gctcatgatg
     3781 gcatcgcaag cccgctccag ttcgacacgg tcgccggctt cggacgcgag ctgtccgaag
     3841 gtcttccggg gtggaagggc gagtccgggc acactctgtc ccaagagttt gtgataggtc
     3901 tcgaactctg cttgtgcttt gtcgagcatg tcgagcttga ggtagatcag aaccaggatt
     3961 tcatgaacct tgggattctc cgggtttgtt tcggccagat cgaggaattt ttccagggcc
     4021 ttcccgtatt cacggttgtt gaagtcctcg acggcctcga atgtcatttc cttatattcg
     4081 tcatagctgt agattcgcgt catgcggttc cctccgggct gcaatccggc aacaaacaat
     4141 ctgggcgcgc ctgtgcagca agataccttc tgaatatcga cactggacaa aaaaagttta
     4201 gccccgcggg tggaggagga tctggcggac cgtcatcagg agttcgctgc cggtaaaggg
     4261 tttttgcagg agtctgggtt ggctcttctc ctggccggtc cgggatctcg agtcaccata
     4321 gccggaaatg aaaagaacag ctgtctccgg atggttggcc tggatctgtt tggcgatttt
     4381 ttcaccggat tggtcgggca gaatcatgtc ggtcagcaga agatcgagcc gtccctgctg
     4441 tgtttgggcc atggccagtc cggtcgagcc gctgtcggct tccaggacga cgtagccgtt
     4501 tctgcggagg aaggttgctg cgagagaccg taccatcgcg tcgtcttcga tcaagaggac
     4561 tgtctcgttg tggacgcctt gggtgtcggt atcaatcggg gatgtttgcg gggtgatcag
     4621 ggacgggtcc gcggcgggaa agaatatccg gaaggtggtt cccgcgcctg ggctgctctg
     4681 gaccgagatg gttccgcccg tctgcgtgat gatcccgtat acggtcgaga gaccgagtcc
     4741 ggttccctgt ccgggcgcct tggtggtgta gaacggttca aaaagatggc ggcgaatgtc
     4801 ttcggacatg ccgcatccat tgtcgctcac gagcaattcg actcccgaca gggtttttgg
     4861 cgtggacgaa ttgcgtatct cgattgtcag cgtaccgccc tcaggcatgg catcccgggc
     4921 attgactgca agattgatga caacctgttg catctggctg gcgtccgcga gaatcggtga
     4981 aacctccggg tcggtattga tgatcagttc gacgttttca ccgatcaccc ggcgcaggag
     5041 tttttccatc tcgctgacaa tcaggaccgg atcgaggagt ttgcgctgca gaacctggcg
     5101 ccggctgaat gccagaagct ggcgggtcag gccactggcc cggtccgcag cctggcggat
     5161 ctgctcgatt tcttcccgtc cagggtattc gtcaggaatc tggagctgcg ccagttcaca
     5221 gtacccgaga atggccgtca ggatgttgtt gaaatcatgg gcaatgccac cggcaagccg
     5281 gcctatggcc tccattttgt gggcctgtcg ctcggaatcc atccgctgct tccgggcggt
     5341 gatattttgg cagaggagtt ggatgcatgg ccgtccgcgg aaggtgatgg ctgtcgcgag
     5401 gatttctgta tcaatcgatc cgccggaggg gaggatcagg gttgtttcga cagcctgcga
     5461 ccagtcggaa ccggcgtccc ggatggcaat cccttcgaag agtcgggagg cgccgggagc
     5521 ccgaccgttc ggggggagat tgaggagccg ttttccggcc gcattcatga agacaatctc
     5581 atcctcatcg acgacaaaga tcgcctccgg cgagagttct acaagttggt ggaattggcg
     5641 ttcggactct ctgcgacttt cctcgtggcg tgcccgttcc tcctcgttcc tgatgaggaa
     5701 aatcatgccg tcatccccgt gcagcgtagt atggcggatc gtcacgtcaa gttgctggcc
     5761 ggaagccgtg atgatcctgg tttcttccgg aagcgtgtcc ggctgtggtg gttgagtgct
     5821 gcccggcagg ctgaagagtt gtgaaagagg cccgccgaaa accgacgaga gtcgacagtc
     5881 aaaaagggct tcaccagccg gattgatgta gtcaatcgag ccgtcagcca ggcagtagat
     5941 cagggcctcc cccatgtttt caaggatcat cgagagacgc cggttatgac tgtcccggtc
     6001 cttctctgtc gaatggcgat agagagcgtt ttcaatgacg atcaccaatt cgcgttcgtc
     6061 acagggtttg cggatatagc cgaaaggtcc ggattttctg gtggactcga tggtttcggc
     6121 gtcatccagc gaggagatga agacgacagg tgtcgcagtt gcacgggcga tctcgtggct
     6181 ggcggtgatc ccgtcgagat cgtcatccag caggatatcc atgagaatca ggtccggcgc
     6241 aagctgtccg gcaagctcga ctgctttatg accaccgctg gctttgccgg cgatagtgta
     6301 tccggcagat aacaacatgt gttcgagggt ggccgaaacg accgggtcgt cttccacaat
     6361 caggatgcgt gccggagtgc tctgcgtcat ggtcccttcc agagttcagg agtacgtgcg
     6421 actggagaat agagtgtacc agagttttgc aaattgttcc agtccccgca atctggcgaa
     6481 gtgccgtgtc agatgccttt gcgggagcgc ttgagatggt acattgcctg gtcggcttca
     6541 ttgatcaggg cgcgaagccg tgtgacaggc ccgcaggcca ttccccagga gagacgcacc
     6601 gtaaacggct cgcgcgaatc ctggcactgg cagattccga gcttgcgtgt gaattgttcg
     6661 cgaacggtct caggttcaac cccatgtgcg ggaatgaaga caagtgccag gaactcgtct
     6721 ccaccgatcc ggaagagcag gtcttcggcg cgcatgcagc ttctgaggga ctgggcggta
     6781 tggcgcagga cgcgatcccc ggcctggtgc ccatgacggt cgttgacttc cttgaaattg
     6841 tcgagatcaa tgacaatcgt cagtgcctgc tcccggatgt ccatcgaacg acgggccggg
     6901 tggtcgctgc tgtcatactg tccgagaatc tcttcaaggc ggttgcggtt gagaagccct
     6961 gtcaggggat cgtggttggc aatcaggatc gcttcctcgt acatctgtgc attggcgaaa
     7021 gcgatggcgg cgaaatcggc gatcgtctgc aaaaccaggt agtcgtcatc tgaaaagagc
     7081 gggccctcct cggtattgat cagttcgatc acaccgtaga ctcgattgcg gaaattgagg
     7141 gggactgcga tgatggaacg tgtcgtgact ccggagatgg catccactct ggcagaaaaa
     7201 agatcggatt tggtcacgtc agggacatag atggcctgcc ctgtctgggc gaccttcccg
     7261 gctatgccct cgcccggacg gagtcggagg tcttccatca gtccagtgtc gattccttcg
     7321 gcgatcatga aaaagagttc gttcgtgttg ggatcaagac gcaggagact ccagttctgc
     7381 ggctcgaagt attcacggat ctcgtgcatg atccggtcaa ggatgacagg gagttcgagc
     7441 gatgacgtga tgagcatgcc gatttgcgag aagagcgcct tgagaggata ttggtttttc
     7501 attgtcgtac cgtgattttg tgctggcatc gcagactccg ggggcccgcc cgggagcatt
     7561 cgttccaggg cgggttgtgt gcgggagtgg ttcagcgctt tcggccgatc ccgtcaaagg
     7621 ctttggcaag cagggcttga tccggtttgc gcgtccagat gctgaccaga gggatgacca
     7681 aaaatggcgc gatcatggct atggtcgagg cgatgggtga attgttgggc cccaggataa
     7741 agaagaggct gatggagaga acaagtcctg tccccatgcc cgcagcaata cccgttctgg
     7801 ttgcgccctt ccagtagagt ccgtagagga atggcgccat gaatgtgccg gcgacggccc
     7861 cccaggagag ggacatcagg gtgacgatca ccgagagttc gtaccgcgcg ataaagaacg
     7921 agatgatgat gaagagcccg gagaggaacc gcatcatggc cacggaattt tctttggaga
     7981 tgttcgggtt gatgtgcccc ttgtacatgt cgatggcgac ggccgaagcc gagaccagga
     8041 ccagggagga aagtgtggac atggatgcgg agaggacgag gagcaggatg accgccatca
     8101 ggagttccgg gagatgtgtg ctgagcatgt ccgggacgat tcggtcataa tcgacttttc
     8161 cgtccttgtt cggcacgaca ggttttgtct tcacctgggt atcggatggc tgtgcggtgg
     8221 cagcagcctg tgttgtagca gttcgcttga gtgtctcgtt gccggccgtg gttgtggaaa
     8281 ctgcagccgg tttttgttgc ggggcttgct gcgccgttgt ctgtacgctc tttttgggtg
     8341 cctcgtaaaa gacatgggag agcgcaccgt tgaaataggc ggcaaagacg atgaccagtg
     8401 aaaagaccgt tgtcacgatg gcggccttga tgatcacgcc ttcgctcttg attgcgtaaa
     8461 acttctggac catctggggc agaccccagg tcccaaagga ggtcatgaag acgaggctga
     8521 agaccatcag ccagcccggt tgcgctcccg agggaacatg ggcggcatat tcgctctgga
     8581 cagcggcgat ggccttgctg actccgccgg cttcaccgac catgatcgcg atcatggcaa
     8641 tcgagccgat gatcatgatg atgccctgga tgaaatcggt cagcgtgatg gcgaagtacc
     8701 cgccaaggat catgtagacc ccggtgattc cgatcatgat caagagggcc acatcgtagg
     8761 acatcccgaa attgatctcg aagagatggc cgagtccttt gaagaccgag gcgcagtagg
     8821 ggagaaggaa gacgaaaatg atgacggcac tgaccatctt gaaggacttg gccccgtagc
     8881 gctcctggag gtactccggc atggtcatca cgtccagatt ttgggtcatg cgtcgggtgc
     8941 gacggccaag gacgagccag gcagccagtg caccgaagaa tgtgttcccg agaatgatcc
     9001 agaggacatc gaggccgagg ccccagccga attttcccgc gaatccgata aacaggacag
     9061 ccgagaagta cgaggtgccg taggccatgg cggagaccca ggggccgatg gaacgtccgc
     9121 ccaggaaaaa gtcggagagg gaactggttt tgcgcatgcc ccagatcccg atgccgatca
     9181 tgacggcaaa gaagacaaca aggaggatga tggcgatcat attgattcct ttgtcaatga
     9241 gatgtgacag tctgaccagt atagcaggat ttgggtgaaa aacggtgatt ttttgttgtg
     9301 atcagttttg tggaagaaca aatacggaaa acattcgacg gatagccagg cgccaggcct
     9361 ggtgaatttg aaagactttc aggatgtgat acaatagagt gatagagccg ggcgggaatc
     9421 tcccgatccg ggcatcttca aaacaaagga actgaatttg tatgaaatac tcaacatgga
     9481 ttgttggtgt ctgcctgctg gcgttcgtgg tgttgccggg ggttctcgcg gcggacgagg
     9541 aacagacggt tgctgcggtt gattttaaaa agcttgtggc tctgctggca actccgcccg
     9601 ccggttttac cgctgtgggc aaggcagagg gcagcaggct cgatatgggc gagggaagct
     9661 ggagtacggc tgttcgtgag ttcaaaaaag gccagaagcg cgtcaagata accattctgg
     9721 acggagcgta tatggaacag gcctacgcgg cgttcaatgc cctcaagatg ttctcgtacg
     9781 agacgagcga cgggtatgtc aagggtgtca cgatcaatgg acatgccggg atcagacagc
     9841 aggagaacag cagtaaaaag catacagtca tgctgctcgt tgccggtcgc ttcctcgtgc
     9901 agtgggaagg gaaaaagctg gcctcgatgg atgaactcga cgccatcgtc aacgggtttg
     9961 atttttcagg tctgacggct ctcaagtgag gcttctctcc accgggtaca accgctctgt
    10021 cacggggcgg tttttttatg tccggggacg cggacaaagc gggccgagat caggttgacg
    10081 accgcaattc cggcgccgac cagaaagacg gcactgtgcc cgagctcatg ccagaggaga
    10141 cccccgatca gggcgatcga aatggaaaag acgtgatcga tcgagacacc catggtgagg
    10201 gtctgttgga catcctcggg agccagggcg atttttttga gccaggtggc ccgtgccatc
    10261 cctgccgaaa cgaggagctg gtccacgacg tagcagatgg cggtcaagac aagcgcaacc
    10321 gagggaatga agaggtcact ggcgaaggcg tatcccaggc agatgacgat caggagaatg
    10381 gcctccacgg taaagacgaa gcgttcgccg cgggtgtcga tcagatgacc gagccagggt
    10441 ttgaaaaaga tcccgatgac gccgccgatg aagagcagga tggcgatggt ttccgtcggc
    10501 tccttgaaga tcgtcacgag aacccagggg gcaaaggtca gaaagagctg tttgcgtgtc
    10561 ccgtagagca cggtcagcca gtagaagagt ccgtattccc gtcggaggcg aaagcggctg
    10621 gacgcggggt ggggttggtc acgggacatc agggcgatca tcagggccgc aaagaagaag
    10681 ccgcaccctc cgatgacaaa acttgtcgta aagtcgaacc cggccaggcg gaatccgccc
    10741 cagaccacca ggctgccggc gagtccggcc atatttcccc aggattgcag tcgcccgagg
    10801 acccgcccgg tatggccttc gggcgccagt tccatggcga tgctggattg gaggggaaag
    10861 aagaggtgct gcccgaccga accgatgaag agccagagaa gcatggtgtt gaagctggac
    10921 gagaagaatg cgatgaggaa cattcccagg ccggtcaatg cgttggctgt gacagccagg
    10981 tgccgcgggc agagaaaggc gaggagtgcc gagacaaaga tgaccagaag gccggggagt
    11041 tcccgcggaa gttcgaggat tccgcgctcg aaattcccca tggtaaaggt tga
//
LOCUS       DAOSHE010000015        10502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5358_length_10502_cov_4.237006, whole genome shotgun sequence.
ACCESSION   DAOSHE010000015 DAOSHE010000000
VERSION     DAOSHE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5358_length_10502_cov_4.237006"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLD82_00940"
     CDS             complement(<1..1019)
                     /locus_tag="PLD82_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63985.1"
                     /translation="MKRLFAVVLISALAAGSLAALDAPQVDILNLRSKAMGGVRLSIN
                     DDQYSIMNNPAGTSLLERSWVSLVQAHFVFSGDFLSLYDHKDSFTGIASGSSDIDNST
                     WNYLSRMKISVGSAPLYFTMLNILPFNINLAVFNSLNVRMKANPDIPVPTWDMLAYND
                     TVLVANIATEVLDIKSIDMALHFGVNGKVINRVQYRKDKMDLFYLYNLGSLDLKEMEI
                     RRGLAFGMDLGFVATFGEKKDLKASLTLSDFYATRFAWTKPTSTDSIGDVVFGDGTDA
                     GAAYIDPSLAIGGSYRVGTIIPVLIENLTVALDIRDLFDPDSNSFLKVYAGFEFSMLG
                     FIKLRG"
     gene            complement(1035..2099)
                     /locus_tag="PLD82_00945"
     CDS             complement(1035..2099)
                     /locus_tag="PLD82_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63986.1"
                     /translation="MMIRRTTAMQIFMLVLTILLASCNVFDPLYSDDGADDFDKALSN
                     AEAAMKRDDFSAAVQLYIEALGKKPNSSEAMIGLSSAIVLRDIQIKDVPTLMNAIFNI
                     DASGTNTNSFLSSLSTPSADYKAKIIAAVSNAAWYRAPVIGIDRVTGSVRLGSNNLPW
                     ALPSESDGVIPSTDRNVTLNYLIIKAVHVALTVQEQFVVTQSLVANIDTDALTTFDAT
                     QLVSTNELANRATFTNFHVSYTNTIASLSNTYLKILGTLTATNNNASLLNLIGVADGM
                     VNLLQSPDSGMSADSVARAVDAVSDIRTSLTNLVNSITNDSTGLKAGFDTLSAFRIQI
                     ENFAKYTNILVVKSSNWMPY"
     gene            complement(2240..3163)
                     /gene="pdxA"
                     /locus_tag="PLD82_00950"
     CDS             complement(2240..3163)
                     /gene="pdxA"
                     /locus_tag="PLD82_00950"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG63987.1"
                     /translation="MPVAVTLGDPWGIGPEVTARAIVADAIRPGQVLVLGPRFAWESM
                     CSLEPGLLAHRDYFLEIATDLHPGAPGKPPTLGGQVVLDTLHRAVDLVNTGDADALVT
                     APLNKSLIHKIDPAFVGHTEFFGQAFGIEDPTMTFVGPEYVTALVTTHVSLRKLADEI
                     TEDRVYRHIMRLWEWLRSHRGDAALLAVTGLNPHAGEAGAFGDEEIRVIGPAIERARS
                     AGARVDGPFPADTVYREAGPGGRYAGVLAMYHDQALVLFKTTDGNQGVNMTLGLPVVR
                     TSPDHGTAYNIAGKGIADFRSMQAALNLVLP"
     gene            complement(3167..3559)
                     /locus_tag="PLD82_00955"
     CDS             complement(3167..3559)
                     /locus_tag="PLD82_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG63988.1"
                     /translation="MAVISKGEITNSIIGEKSFFEGRFMVKGALRVDGKFEGDALEVD
                     QLFIGQTGRIKTNINAVNVVVEGLVIGNIKASTRVMLLPTARILGDIRTPELIIQNGV
                     ILEGRCIISNDLNQSARTLIENLYASTD"
     gene            complement(3597..4412)
                     /locus_tag="PLD82_00960"
     CDS             complement(3597..4412)
                     /locus_tag="PLD82_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63989.1"
                     /translation="MDSGTKSLVEEKISLLSRQLEMVLRTSGNPDQKKRVSQQLAALK
                     KDRLRLDEGSFGEDDIRRYLGDVSAQQVESDEEEGSEETVGRFPLLSAVMIQPASPEC
                     NEQEVNEIASFLRHFEREYLSVLGNYQLKLDFNHAQKRDTFFNLYGAIQHSLKSYLDV
                     VHDISSGRVASGEHADSLRQMKRKLFMDLIMKTSDFTHGLHDFLSLLISDYQNGGNLV
                     LNPEQVLVFDPLHGHRELDGMQIAEGIERIRLFLDEFSEYLNVPGLKKMQNRP"
     gene            complement(4412..6196)
                     /locus_tag="PLD82_00965"
     CDS             complement(4412..6196)
                     /locus_tag="PLD82_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG63990.1"
                     /translation="MEPMMTGLIWEDLACEPLVWDVAMTEFHRITLDGETVPGGPSDD
                     SLADGTAPVMEDQPGSESAKTDDRESFVHGIARWTQLEMESMFLREVARSLSFPLRVN
                     TLVKLPNGRLPRVIERLVPRLESFVQHGYSLRGDTIACMLKVFLVAGEDERARRLAES
                     LSSQVDSAEIRYYHGLALFRLSLHDQACAQAKRAVSMDPASAISHLLLAACQIKTGEV
                     HEAGRTLAGALPLFARDEAVLRFAIPWIRKTGDEDALVQVLSSVTDLRPDDVESQLVL
                     ADILYRRGQFGSICEQYGKYRDSLDRARLCRYGICLASLGREQEALEIFSSLLSHQPD
                     HPDLWSVVQALYDSGLRDAGLVRLIAQRLMDCGRVEECLELCSRHADLAGGDPGLVFL
                     QVQCYTQRGSYEKALALLGNMPAGSSASEDERAYLTGYLLFLLGKREEAVPWLESGCR
                     MPGREVAALRMLGEIAVHSGDHHKAAGIYRKLSRLLRDDAGVLARAAWSSMQAGMVSL
                     AIEGFRDLVRVLPDCPEGWNNLGVLLARSGQFEEAARAFKTSLRLDPDQPEAGKNLAL
                     VYDRVLRSKSAEYLERFADGELSTAGGD"
     gene            complement(6278..7099)
                     /locus_tag="PLD82_00970"
     CDS             complement(6278..7099)
                     /locus_tag="PLD82_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOG63991.1"
                     /translation="MKTIQRVGVLILLLVAFLTLGTLGVAQSPTGGNGATNPAGDDND
                     PKGTADEQLVTFILDDFEKAASWIPEMPRDQGVIFSMQRPGTPQAVSNMLKQITDQNQ
                     NYLKGLADQGKQNSENPLVGFPYNQNKFVLGVRVEFMKRGYNWFTVRPPLPIKVPGVC
                     KSLSVYVAGRGYNHSLWVMIRDYEGNKRFLSAMRPLNHKGWRRVLFPIKPNIVQEDYK
                     VTDPKKIGITFDGFLINCDPMESQGKYYIYFDFLTAQVDLYFDSNGDKDDMRDFW"
     gene            complement(7122..7892)
                     /locus_tag="PLD82_00975"
     CDS             complement(7122..7892)
                     /locus_tag="PLD82_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOG63992.1"
                     /translation="MKRTGKLCTLFMLVMLLTIVGLVGDSSLTAQFSGGPVNNTQNIQ
                     TFVLNSFEDPKEWRVDYSMYAAKKYNPSTKQFEIDRERNIWTNFVGKPWGVTVEGTNN
                     RCLAVKASYDRKGYNWMEVYPAKPLPMIGRVEAIDVWVWGGNFQWRLEIHLLDYLGYK
                     HIIDAGWLNFIGWRNIRLAVPRHIPQGERYVPRLKTLRFEKFVLFANPGEREDVFYVY
                     FDRMQIQADVYSERFDGDDLVVKGTNIGWMPKQETLKQ"
     gene            complement(8046..8402)
                     /locus_tag="PLD82_00980"
     CDS             complement(8046..8402)
                     /locus_tag="PLD82_00980"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOG63993.1"
                     /translation="MQYQMLQAKIHRATVTDSDLQYEGSITIDLDLIEAAGMREYQKV
                     QVVDINNGSRFETYIMNGSRGSGTVCINGAAARLVHRGDLVIIFAYCLYDETELDGYA
                     PKIILVDDNNRIRQVH"
     gene            complement(8377..9315)
                     /locus_tag="PLD82_00985"
     CDS             complement(8377..9315)
                     /locus_tag="PLD82_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG63994.1"
                     /translation="MAITVVGSVALDSIQSAAGSVEREIGGSAVHFANAASLCSKVYI
                     VGVVGDDYPFDRIRYLKDRGVDFDGVEIVPGGKTFFWKGRYEGDMGSAISEATELNVF
                     QDFAPVVKGEARKSRFLFLANIHPGLQLHVRKSIGDGAFAVLDSMNYWIENAREDLLG
                     VIAAVDAAILNSDEIRSLTGEKNLIAAARIVLGMGPSYVIIKKGEHGVLAVSRDWIAV
                     LPAVPLETIVDPTGAGDSFAGALVGYLDRAGSMDAATWRKALAWANCVASFNVQDFSV
                     YGIAGITVRDVVRRYDEYRKVFQIEGELDAVSDVAG"
     gene            9472..9939
                     /locus_tag="PLD82_00990"
     CDS             9472..9939
                     /locus_tag="PLD82_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63995.1"
                     /translation="MNVRSFLITAGLLLLCLGSCSHDSGEKSRFVAPLDTISLGMKRS
                     EVERRLPLSSGTWQSATLFSSLLGITESTLTNRGLVNVYLFYRKDGDENLLNAAVLEF
                     SLREETRLLAEADRRYGTNRNRIWQPGEAIRIELEPPRSGRVKMIYDTTSPHR"
     gene            complement(10165..>10502)
                     /locus_tag="PLD82_00995"
     CDS             complement(10165..>10502)
                     /locus_tag="PLD82_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63996.1"
                     /translation="LLKNTPFVGSASNRLVHRTDSNCPRALEISPNERWYIKDIPHDG
                     NYIMCPFCFPQPKDEEESEIVVSETQAPAKVRRKPAPKVEGETAAKPKKVAAKTRVSA
                     TSKKKTAKK"
BASE COUNT         2516 a         3043 c         2636 g         2307 t
ORIGIN      
        1 ccacgcagct tgataaaccc gagcatcgag aattcaaatc cggcatacac cttgagaaac
       61 gaattgctgt ccggatcaaa gagatcccgg atatccaggg caactgtgag attctcgatg
      121 agaaccggga tgatcgttcc aacccggtac gagccaccaa tcgccagact ggggtcgatg
      181 taggccgctc cggcatccgt tccatctcca aagaccacat cgccaatgct gtccgtactt
      241 gtgggcttgg tccaggcaaa gcgcgttgca tagaaatccg agagcgtcag ggatgccttg
      301 agatcctttt tctcgccgaa ggtagcgacg aagccgaggt ccataccgaa ggccaacccg
      361 cgtcggatct ccatctcttt gagatcaaga cttccgaggt tgtagagata aaagagatcc
      421 atcttgtcct tgcgatactg gacacggttg atcaccttgc cattcacgcc aaaatgaagg
      481 gccatgtcaa ttgacttgat gtccaaaacc tctgtcgcaa tattggccac cagcactgta
      541 tcgttgtagg ccaacatatc ccaggtcggg accggaatat ccggattggc cttcatccgc
      601 acattcaacg aattgaagac tgccagattg atattgaatg gaaggatgtt gagcatagtg
      661 aagtacaacg gcgcagatcc caccgatatc ttcatccgcg agagatagtt ccaggtcgaa
      721 ttgtcgatat cggaactccc cgaagcaatc cctgtaaagg aatccttgtg gtcatacaag
      781 ctcaaaaaat ctcccgaaaa cacgaagtgc gcctgaacaa gcgaaaccca gcttctttcc
      841 agcagggagg tcccggccgg attgttcatg atggagtatt ggtcgtcgtt gatggaaaga
      901 cggacacctc ccatcgcctt cgaccgcagg ttgagaatgt caacctgcgg agcatcgaga
      961 gccgcgagcg atccggcagc caatgcactg atcagtacta cggcaaacag tcgtttcatg
     1021 cgtgccctcc tttgttagta tggcatccag ttggatgact tgacaaccag tatatttgta
     1081 tacttggcaa aattctcgat ctggatgcgg aaggctgaaa gggtatcaaa ccctgccttg
     1141 agtccggttg agtcattggt gatcgaattg acaagattgg tcaggcttgt gcggatatcc
     1201 gaaacagcat ccaccgccct ggctaccgag tcagccgaca tgcccgagtc gggcgactgg
     1261 agtagattga ccatcccgtc agcaactcca atcaaattga gcagggatgc gttgttgttg
     1321 gtagcggtca aagttccgag gattttgaga tacgtattac tcagggatgc aattgtattg
     1381 gtatacgaga catggaagtt ggtaaatgtc gcccggttgg ccagttcatt agtcgacaca
     1441 agttgtgtcg cgtcgaaggt cgtgagtgca tccgtatcga tattcgccac tagactctgg
     1501 gtcaccacaa actgctcctg aacggtcaag gcgacatgga cggccttgat gatcagatag
     1561 ttgagggtca cattgcggtc cgtcgaagga atgactccgt cactctccga aggcagcgcc
     1621 catggaagat tgttggaccc gagacggaca gaaccggtca ctctgtcgat gccgatgacc
     1681 ggagcacgat accaggccgc attggatacc gctgcaatta tcttggcctt gtagtctgct
     1741 gatggcgttg acagactcga gagaaatgaa ttggtattgg tcccgctggc atcaatatta
     1801 aagatggcgt tcatcagggt gggcacatcc ttgatctgga tatccctgag aacgatggcc
     1861 gaagaaagcc cgatcatcgc ctcggaagaa ttcggttttt tcccgagcgc ttcaatgtac
     1921 aactggactg ccgccgaaaa atcatcccgc ttcatggcgg cttcggcatt cgaaagggct
     1981 ttgtcaaaat catcagcccc atcatcagag tagagcggat caaagacgtt acaggaagcg
     2041 agcaagatcg tcagcaccag catgaatatc tgcatcgcag tcgtccgacg tatcatcatg
     2101 gctgtccctc ctttgggggg tacatgcgcc gcagcaacaa cgttaccgta cttcgcggtc
     2161 aaactcaagt atactccaga catgtctcat tttcagcatt tggtctttca aaaaatatca
     2221 taggataatg cagcttcttt tatggaagca ccaggttcag tgcggcctgc atgctgcgga
     2281 aatccgcaat tcccttacca gcgatgttgt aggcagtgcc atgatcgggg gatgttcgaa
     2341 caactggcag tccgagagtc atattaacgc cctggttccc gtccgttgtt ttaaagagaa
     2401 caagcgcctg gtcatggtac atggccagca cgcccgcgta ccggccaccc ggaccagctt
     2461 cccgatagac agtatccgcc ggaaacggac catccactct tgcgccagca gaacgtgccc
     2521 gttcaatagc gggcccgatc accctgatct cctcatcgcc aaaagctccc gcctccccgg
     2581 catgcgggtt caggccggtc acggccaaaa gggctgcatc tcccctgtgt gacctgagcc
     2641 actcccagag acgcatgata tgcctgtaga cacggtcctc cgtgatctcg tccgccagct
     2701 tccggaggga aacgtgcgtt gtcaccaggg cggtcacata ctcgggaccg acaaaagtca
     2761 tggtaggatc ctcgatgccg aaagcctgtc caaaaaactc ggtatgcccg acgaacgcag
     2821 gatcgatttt gtggatcagg gatttgttga gcggcgctgt caccaaggca tcagcatcgc
     2881 cggtgttaac caggtcaacc gccctgtgca atgtgtccaa aacgacctgt ccgccgagcg
     2941 tcggcggctt tcccggcgcg ccgggatgaa ggtccgtcgc tatctcgagg aaatagtccc
     3001 ggtgcgcgag caaaccgggt tcgagagagc acatgctctc ccatgcaaac cgcggtccga
     3061 gaacaagaac ctgtcccgga cggatagcat cggccacaat ggcccgggcc gtgacctcgg
     3121 ggccgattcc ccaaggatca cccagagtta ccgcaaccgg catgactcag tctgtcgatg
     3181 cgtagagatt ctcgatcagg gtccgtgccg actgattgag gtcgttggag atgatacagc
     3241 gtccctcaag aattacgcca ttttggataa tcaactccgg ggtcctgatg tccccgagga
     3301 tgcgggccgt cggaagaagc atcaccctgg ttgaagcctt gatgttccca atcacaagac
     3361 cctcaaccac aacattaacc gcgttgatat tggtcttgat gcgaccggtc tgaccgatga
     3421 aaagctggtc gacctcaagc gcgtcgccct cgaattttcc gtcgactcga agagccccct
     3481 tgaccataaa acggccttcg aaaaacgact tctccccgat gattgaattg gtgatctcac
     3541 ccttggaaat gacagccatc tggcactcct tgccttgcct ccgcgccgtg gcggggttac
     3601 ggtcggttct gcatcttttt cagacccggt acattaagat attcagaaaa ctcgtccaaa
     3661 aacaagcgga tccgttcaat accctccgca atctgcatcc cgtccagctc acgatgccca
     3721 tgcaagggat cgaacaccaa gacctgctcc ggattcaaca caaggtttcc cccgttttgg
     3781 tagtcactga tcaaaagcga caaaaaatca tggagtccat gggtgaaatc actggtcttc
     3841 atgatcaggt ccataaaaag cttgcgcttc atctgtctca aactgtcggc atgctcgccg
     3901 gacgcaacac gtcccgacga aatatcgtgg acaacatcga gatagctctt gagggaatgc
     3961 tggatggctc cgtagagatt gaaaaacgta tcccgctttt gggcgtggtt gaaatccagt
     4021 ttcaactggt aattccccag aaccgagaga tattcgcgtt caaaatgcct gagaaagctg
     4081 gcaatctcat tcacttcctg ttcgttgcac tccggactgg ccggctggat catgacagcg
     4141 gagagcaggg gaaaccgacc gaccgtctct tcgcttccct cctcctcgtc gctctcgacc
     4201 tgctgtgccg aaacatctcc caggtagcgc ctgatgtcat cctcgccaaa gcttccctcg
     4261 tccagcctga gccgatcttt cttcaaggcg gccaactgct gcgaaacacg ctttttttgg
     4321 tcgggattcc ccgatgttct cagcaccatc tccagctgcc gggagagaag gctgattttt
     4381 tcctctacaa gagactttgt gccactgtcc atcaatcccc cccggcggtt gagagttcac
     4441 catcggcaaa cctctcaaga tattcggccg acttgctccg caaaaccctg tcatagacaa
     4501 gtgccagatt cttgccggct tccggttgat caggatcaag ccggagcgat gttttgaaag
     4561 cgcgggccgc ttcctcaaat tgaccggatc gtgcgagcag caccccgagg ttgttccagc
     4621 cttcaggaca atcgggaaga acacggacca ggtcccgaaa tccctcaatg gccagagaaa
     4681 ccattcccgc ctgcatggaa gaccatgcag cccgggccag gacacctgca tcgtccctga
     4741 gcaaccgcga aagcttccga tagatgcccg cagccttgtg gtggtccccg gaatgaaccg
     4801 ctatctctcc aagcatccgc aaagccgcca cctccctccc gggcatccgg catccggact
     4861 cgagccaagg aacagcttcc tcccgtttcc cgagaagaaa aaggaggtat cctgtcagat
     4921 aggcccgttc atcctcgctt gcggagcttc ccgccggcat gttcccgaga agtgccaaag
     4981 ccttttcata gcttccacgc tgagtgtagc attgcacctg aagaaataca agtccgggat
     5041 cccctccggc aagatctgca tggcgtgaac ataactccag acattcctca acccgtccac
     5101 aatccatcag tctctgggca atcaaccgca caagtcccgc atcccgcaag ccggaatcgt
     5161 acaacgcctg caccacagac caaagatccg gatgatcagg ctgatgtgac agaagactag
     5221 aaaaaatctc cagagcctcc tgctccctcc ccaaactcgc cagacaaatc ccgtaccgac
     5281 agagtctcgc cctgtcgagg gagtcccgat acttcccgta ctgctcgcag atactgccaa
     5341 actggccgcg tctgtacaga atatccgcca atacaagctg cgattcgaca tcgtccggcc
     5401 tcaagtcagt cacgctcgac aggacctgaa caagggcgtc ctcatcaccc gtcttcctga
     5461 tccatggaat cgcgaaccgc agtacggctt catcccttgc gaagagcggc aaagctcccg
     5521 ccaatgtcct tccggcttca tggacttcac ctgtcttgat ctggcaagcc gccagcagga
     5581 gatgggagat tgcactggca ggatccatac tgacagcgcg cttggcctgg gcacaggcct
     5641 gatcatggag actgagacga aaaagcgcca gcccgtgata ataccggatc tcggcgctgt
     5701 caacttgaga cgataatgat tccgccaagc gtctggcacg ctcgtcttca ccggccacga
     5761 gaaatacctt gagcatgcac gcgatggtat cccctcgcaa ggaataccca tgttgcacaa
     5821 acgactccag cctcgggaca agccgttcaa tgacacgagg gagtcgccca ttggggagtt
     5881 tgacaagcgt gttgacacgg agcggaaacg agagggagcg cgcgacctct cgcaaaaaca
     5941 ttgattccat ttcaagctgg gtccagcggg caatcccgtg gacaaagctc tccctgtcat
     6001 cagttttcgc gctctccgag cccggctgat cctccatcac cggagcagtg ccatccgcca
     6061 gcgaatcatc cgatggccct cccggaaccg tttctccatc cagcgtgatg cgatggaact
     6121 ccgtcatggc gacatcccag acaagaggct cgcaggctag atcctcccat attagaccgg
     6181 tcatcattgg ttccatgtgc aactccctca aacacgaagg ccacacacca gtagactggg
     6241 tgcggccttg acagcgctct ctgatgaagg aagaatatca ccagaaatcg cgcatatcgt
     6301 ccttgtcccc attggaatcg aagtacagat ccacctgggc agtcaggaaa tcgaagtaga
     6361 tgtagtactt gccttgagac tccatcgggt cgcagttgat gaggaatccg tcaaacgtga
     6421 tgccgatttt tttggggtct gtgaccttgt aatcttcttg cacaatattg ggtttgatgg
     6481 gaaagagcac cctgcgccag cccttgtggt tcaagggacg catggccgaa aggaagcgct
     6541 tgttgccctc ataatcccgg atcatgaccc agagcgagtg gttgtagcca cgaccggcca
     6601 catacaccga aagggatttg cacactccgg ggaccttgat ggggagcgga ggtctgaccg
     6661 taaaccagtt gtatccacgc ttcatgaact ccacccggac accgagaaca aacttattct
     6721 ggttgtacgg aaatccaacc agtggattct cggaattctg cttaccctgg tcagccaacc
     6781 ctttgagata attctggttt tgatcggtga tttgcttgag catattgctc acagcctgcg
     6841 gcgtacctgg gcgctgcatc gagaaaatca cgccctggtc cctgggcatc tcgggtatcc
     6901 aggaagctgc cttctcgaaa tcatcgagaa tgaacgtgac aagctgctcg tcagctgtac
     6961 ctttgggatc gttgtcatcc ccggctgggt tggtcgcacc atttcccccg gtcggactct
     7021 gtgcaacacc cagcgtacca agtgtgagga acgcaaccag aagcagtatc agcacgccaa
     7081 ctctctgtat cgtcttcatg taggaaatct ccttcccggg attattgttt cagcgtttct
     7141 tgcttgggca tccagccaat attggtaccc ttgacaacaa gatcatcacc atcaaagcgt
     7201 tcggaatata catccgcctg gatctgcata cggtcgaagt agacgtaaaa gacatcctca
     7261 cgctcgccgg gattcgcaaa caaaacgaat ttctcaaatc gcagagtctt gagtctggga
     7321 acatagcgtt caccctgtgg aatatgccgg ggcaccgcaa gccggatatt gcgccagcca
     7381 atgaaattca accagccggc gtcgataatg tgcttgtagc cgaggtaatc cagaagatgg
     7441 atctcgagcc tccactggaa attcccgccc cagacccaga cgtcgatcgc ttctaccctg
     7501 ccgatcatcg gcagtggttt ggcaggatag acttccatcc agttgtatcc cttgcgatca
     7561 taactggctt tcacggccaa acagcgattg tttgtgccct cgactgtcac accccacggc
     7621 ttcccgacaa agtttgtcca gatattccgc tcgcgatcaa tctcgaattg ttttgtcgag
     7681 ggattgtact tctttgcggc atacatcgag taatccacac gccattcctt gggatcctcg
     7741 aatgaattca gaacgaaagt ctggatgttt tgggtgttgt tgaccggccc gccactgaac
     7801 tgcgctgtga gcgaggagtc accgacaagc ccgacaattg tgagcagcat gacaagcatg
     7861 aagagtgtac aaagttttcc ggttcgtttc atcgctatcc cttcttccct cgtccaccat
     7921 gtacgcaaac ggacgggacc atacctaaag gtgtatcgtt attcctatcg gcaagtatat
     7981 ccgaaaaatt cagtgctttg caacgaattt gcaagccgac caagccacag aacgggcgtc
     8041 cgtcctcagt gaacctggcg gatccggttg ttgtcgtcaa ccagaatgat ttttggggca
     8101 tatccgtcca gctcggtctc atcgtacagg caataggcga agatgatgac cagatctcca
     8161 cggtgcacaa gccgggcggc ggcgccattg atgcagaccg taccgctccc ccggctacca
     8221 ttcatgatgt atgtctcgaa acggctgccg ttattgatat ccacaacctg caccttttga
     8281 tactcccgca taccggcagc ctcgatcagg tcaaggtcaa tggtgatact tccctcatac
     8341 tggagatcgc tgtcggtcac cgtcgcacga tggatcttag cctgcaacat ctgatactgc
     8401 atctaactca ccctcaatct ggaacacctt ccggtattca tcatatcttc gtaccacatc
     8461 cctgacggta atcccggcaa tcccgtagac tgaaaaatcc tgcacattga acgatgccac
     8521 gcaattggcc caagccaagg ccttgcgcca ggttgcggca tccatgctgc cggccctgtc
     8581 aaggtagccg acaagcgccc cggcaaagga atcaccggcc ccggtcggat caacaatcgt
     8641 ctcgagtggc acggccggaa ggactgcgat ccagtcccgg gaaacagcaa gcactccatg
     8701 ttcgcccttt ttgatgatca cataggacgg acccatcccg agcacgattc tggccgctgc
     8761 tatcagattt ttctcgccgg tcaggctgcg gatctcgtcg ctgttgagga tggccgcatc
     8821 aacggctgca atcacaccaa ggagatcctc ccgggcattc tctatccagt aattcatcga
     8881 atcaagaacc gcaaaggcgc catccccgat gctcttccgg acatgaagct gcaatcccgg
     8941 atgaatattg gccaggaaca gaaaccggct tttcctggct tcacccttca caaccggtgc
     9001 aaaatcctgg aacacattga gctcggttgc ctcgctgatg gccgagccca tatcgccttc
     9061 gtaccgacct ttccagaaaa aggtctttcc accgggaaca atttcaaccc cgtcgaaatc
     9121 gaccccacga tccttgagat agcggatccg gtcaaacggg tagtcgtcgc caacgacgcc
     9181 cacgatgtag accttcgaac agagtgaggc cgcgttcgca aaatgaacgg ctgaaccacc
     9241 aatctcccgt tcaacacttc cggcagcgct ctggatggaa tccagtgcaa ccgagcctac
     9301 aaccgtgatt gccattccgt gacctctcct ggatgcaatg cccttgctgg gcagcccatg
     9361 tattaatact tttattcggg gcgcgaaaca atcaaaaatg tcacgcctgc aaaccggacg
     9421 agtgcgggga agaccctgcc ggccgttcaa gaaacatata aagaggttcc catgaacgta
     9481 cgctccttcc tgatcacggc cggccttctc ctgctctgtc tcggctcatg cagccatgat
     9541 tccggcgaga aatcgcgttt tgtcgccccc ctggatacca tctccctggg aatgaaacgc
     9601 agcgaagttg agcgacgact tcctctctcc agcggaacgt ggcagtccgc cacgctcttt
     9661 tcaagtttac tgggcataac ggagagtact ctgacgaatc ggggactcgt gaatgtctac
     9721 ctcttctatc gaaaggacgg cgatgaaaac ctgttgaatg cagcggttct tgaattctcg
     9781 cttcgggaag aaacacgcct gctcgctgaa gccgatcggc gctacggtac aaaccggaac
     9841 cggatctggc aacctggaga agcgattcgc atagagctgg aaccaccccg gagtggccgg
     9901 gtaaagatga tctacgacac cacctcgccc catcgatgac accctgttga catgacaccc
     9961 gccacgtgcg gctgccacca cggagcaacc acaagtcgat gggcatttct catatatcca
    10021 tagccaaaaa aaccgtttgt tatggaaacc attccaggaa atccggaaga accgctggat
    10081 cccaaaatca acccaatacc ggcggcgagc ccggacttcc gtcagagcac tccagtgatc
    10141 agggagacct gacagggtga caggctattt ttttgccgtc ttctttttgg atgttgccga
    10201 aacccgggtt ttggcggcaa cctttttggg cttggccgcc gtttcacctt cgaccttggg
    10261 tgcaggcttt cgccgtacct tggcgggagc ttgagtctct gaaaccacaa tctcggactc
    10321 ttcctcgtcc ttgggttgtg gaaaacagaa cgggcacatg atgtaattcc cgtcatgcgg
    10381 aatatccttg atataccaac gctcattagg tgatatttca agggcccggg gacagttgga
    10441 gtcggtccga tgaacgagcc gattggacgc tgagcccacg aacggtgtgt ttttcaagag
    10501 ag
//
LOCUS       DAOSHE010000016        10442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5400_length_10442_cov_3.377587, whole genome shotgun sequence.
ACCESSION   DAOSHE010000016 DAOSHE010000000
VERSION     DAOSHE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_10442_cov_3.377587"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLD82_01000"
     CDS             <1..197
                     /locus_tag="PLD82_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG63997.1"
                     /translation="GTREIVFDLKDVPHIGSAGIGKLLLFYKDISTANGVMRVENAQP
                     AVYDLMKMVKLDSVFSIAAL"
     gene            363..1085
                     /locus_tag="PLD82_01005"
     CDS             363..1085
                     /locus_tag="PLD82_01005"
                     /inference="COORDINATES: protein motif:HMM:NF027727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOG63998.1"
                     /translation="MKKMTKVLLVVLLALGLSTTAFAGASGLSIEQHADFDREIFTTT
                     FTGWQADSTGGWFFFLDIDSAAGYKLGHTFNYFEINRDFSFRKLFGDEGLNPLNLHLE
                     YNASTHLDTSYFLTGLKYEISGYPYIFTISASAKLPKNSKGEEPDASWQATAVWSLNF
                     ADGAFVFTGFVDVWNNTEIPGGKLAFISEPQVLVNLGKLGLGKAMESFWIGTEFEISK
                     NFVWWDGDDIFVKPTVFIRYDF"
     gene            1226..1654
                     /locus_tag="PLD82_01010"
     CDS             1226..1654
                     /locus_tag="PLD82_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63999.1"
                     /translation="MTLELDKKQYENLLKLVSLGNAMANSYREETIEEFQGIFDLLYS
                     KAEEAGLGEIVEKDEEGSFFPTEDFELMLNSFVEEYDESCFWDRLIDTLVDRDLVAKF
                     GEEGFEKLSEEELETEQEKLIKFYATEFEANDVENLFLKK"
     gene            complement(1701..3122)
                     /locus_tag="PLD82_01015"
     CDS             complement(1701..3122)
                     /locus_tag="PLD82_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG64000.1"
                     /translation="MIRRQVAEESILFFSILKWFFLAMVSGASVGLVVGCFMLALDAA
                     IGSVQVIPNYYYLLPLALVCTSWVSRRVVPLADGYSTNRVIASIHKNVLVHPFSVVKA
                     FFLPIITIACGGSAGREAPGADIGAGLSAGLSRLLRLDPADRRKLMICGISAGFAAVF
                     GTPISGAIFGVEVLFVGALLYEVLFPSFVAGIIGYQVASAIGVVYVHTPIKFVPLFTE
                     DFFLKVVVAGIFFGLVTVLVIETLRATRRFLEKHARRLVIRALAGGVLIVLLVELTSE
                     KYLGLGVAQMALYTSGQPAEWFDFLLKLVFTALTLQAGGVAGLVTPVLFIGAASGSFF
                     AKFYTISSTHLGGLYLADLLKFQNPVFPALGMVSLLAGAANTPLSASIMAMELFGPEI
                     APYAAVTCLISFLMTGNRSIYPAQVLVIKKTRSLGVQHGREMGDVHQRYYLHDTRVAG
                     GLWLFIRGMKKALRRMFSKRRRR"
     gene            complement(3119..4420)
                     /locus_tag="PLD82_01020"
     CDS             complement(3119..4420)
                     /locus_tag="PLD82_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64001.1"
                     /translation="MKIDKSMHQPESDCHVQRFVRGDGQALPRISRWRERAGFVFRKI
                     LARKIVLRILLLFCSARIIPRRYLVRYFRSPGVLVEVEDVLGKVRSIGGWSRAWANLA
                     AGRVALARDACQSGWYETACGHELAAALYYHGASSTLCTSGEIRLRNMERADTAMTRA
                     MQLVHERSAGVSLGIGDALLVRIPWKGTSFNGWLLLPPRSVRDGLRTTVVIPGGADSG
                     KEEYLQYARHFLVRGFAVLVMNDPGVGETARMLPLEPDGGALYDAVRESLVRAASRYV
                     TGESFWLGASLGGWKVLQVGVHRGRADGLLGIVSISGPYCPARYYGRLVFAIHEMVRF
                     ALGVRSSGRIRAVLRLYSLETTIGAISVPSLVVVGGRERIIPASDGIRLYTDIGGSDV
                     AMYYLPGSDHVCMDGVDLVLPDIADWMYARCPRRMIQEEGS"
     gene            complement(4427..7690)
                     /locus_tag="PLD82_01025"
     CDS             complement(4427..7690)
                     /locus_tag="PLD82_01025"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOG64002.1"
                     /translation="MTTQHLERVRHLLTQAGIEAGASDEDVRNGKRLLHEGSVLSFES
                     GPGVFSCRVADERLHDVRLEEQGTCLEGTCTCGQATGRCAHVIAALYALKKRLPARAS
                     AVGSPGKARGVLEFFRESMHFVPPSGSVSPGLDAILDLRGLFRKERSCTVQLRIQASD
                     GLLVPDPGAFLDAWKARSQMRITDAVLYDPSIWLLDAGEERLLGCLWQLQDAGAALPV
                     QRGLQLTAAALPGVLDALAGTRVGVRAGGDDVKEYFVDGDALNCEYRVRDLSGGDLEI
                     GFVLHNGEFHCSSSAISLLPLQPVWAVAGDRLVRVDTLLALSMQNALHQYTGMPERIT
                     VQRVEATTVLEELLPFLERRGRLTMEGRMAKCIKQANRPPRPVVRVDMHELLIRLDLD
                     LGFRYGRERVAWNHPGRLVQSGEYSWQIRRQQDEQHIVDLIVAAGFRADDDGHFVLQD
                     EQKFFEFVYFVLPEHAGQWDVYYNAAFKQAARRLSRPRVGVKLSKKYDFLELSIFPRE
                     RGLALDPMAVLESARAGKGYVRLSNGQFLPLSDEQKALLRELAGRVDFSPARRDGRNI
                     LTAESIFAPVVAETMRENAPKEDGDIEGDASLEGFSESLVSWKKRVTIPEGINATLRE
                     YQHDGLSWLANIYTNSLGGILADDMGLGKTLQTITFIQYAVTAGEKDPFLIVCPSSLV
                     FNWVAELSRFAPALAVLSVGGTPLARQEMYKELFMFHVVVISYNLLQKDQEFLENREF
                     ACIFLDEAQHIKNPGSIRTISAKAIRARSRYALTGTPIENCLGELWSLFDFVLPGYLF
                     TYQRFKDEIEKPIMEGSSDEALATLKSRIAPFMLRRTKDEVLTQLPPKTEQVSICPMF
                     DEQKEAYLSVLSFFDSSLLPAIEKDGVQKHSLELLAALTRLRQICCHPGLALEKYGWA
                     TSGKMELLSEIMNQAVDGGHKTLIFSQFTGMLDLIEQRLGAEGFSVCRLDGSTNVKHR
                     GEIVKRFNEDSGMNAFLISLKAGGTGLNLTTADTVIHVDPWWNPAAEDQASARAWRMG
                     QTRPVMVYKLIAEGTVEEKILALQQDKKRLVQAVIGDSPVNLSRLTLDQIKDLFKRE"
     gene            complement(8011..8847)
                     /locus_tag="PLD82_01030"
     CDS             complement(8011..8847)
                     /locus_tag="PLD82_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64003.1"
                     /translation="MKSCLMIVLVAGMLCIGGTAFSAEDIPNFSTIGLTLVSQTNQED
                     GRVVASLKDASGRECSISWESAPDAANLRKIAELRDIVHSWAGLEVRTLSIILSGQTV
                     EAVVVPERLTIEGTDVARFMPAGIGFYYREYVQFDFRMVKDSISMRIAGRFIDRVGLA
                     TLIQRAIDNPLTFADKTNYMARLDRLDRLLEELVARNARLEREHQLLRHATLRLFNTG
                     FLCGPDEIPEKSVKRAVAIKTANPKFDADALRDALRKEGIKLSGGEVELILAIFFNEW
                     DD"
     gene            complement(8881..10236)
                     /locus_tag="PLD82_01035"
     CDS             complement(8881..10236)
                     /locus_tag="PLD82_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64004.1"
                     /translation="MGNDNKVSRGLTRKTEVFFRENAVETMDRPVLYSLEDEFAKTKK
                     RKHLFVYAGAVLFAGALVVVTLVITGEVRRQSDAIKVDVSDFRDLNLTDLLQNTKKDE
                     TRLRSLMQEIADFRGNWQMEAQKVRDRYITERDIAAIRYADEADRQRALAVVQANERA
                     EMGTLDARYQKAIAERQKEIDEITKRLAGRDKKMVQGAQAMEMVLGSQLKLIEIERKK
                     IEDQWRQRLDEAQNRFRLEAAEQRRLQKKLTDSLVLRYNPVFGDALMQGLIAGNSRLS
                     GPGTQHAWQAILAREGVLDQAGFASLRKNIRDERTILERIIANRYTNSITPALNALGN
                     RQAAVVAGYEALWSGLAARIEQKNSRLDSYNRAFAALMQQTREHGYILDTLRPDAYPV
                     IVNPAYQVKSGDMAFVIRGENTRIGMVRLERSRDGLIAIPEGAFSDKPAALDKLLLQL
                     R"
     gene            complement(10215..>10442)
                     /locus_tag="PLD82_01040"
     CDS             complement(10215..>10442)
                     /locus_tag="PLD82_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64005.1"
                     /translation="GRRIYYPVEAANVNRSMKTAFAQYEEALELYYKGNWKRANALFK
                     SCSFEPAAIFATRTQGTCPRNWSGIWEMTTK"
BASE COUNT         2408 a         3180 c         2735 g         2119 t
ORIGIN      
        1 ttggcacccg cgaaatcgtc tttgacctca aagatgttcc ccatattggc agcgcaggca
       61 tcggaaagct cctcctgttt tacaaggata tttcgaccgc caacggggta atgcgcgttg
      121 aaaacgccca gcctgctgtc tatgacctca tgaagatggt caagttggat tcggtcttct
      181 cgattgcagc gctctagacc ggagagcggc gggctctgtc ctgacgggtt cttccacgcc
      241 gaccggaagg tactgctgac ggcaacccgc ccggtccatg atgtaccgta aattatcttg
      301 catcccgaca cgaatcgggt atactgacat ccggttgtac caagttcgaa aggagctctt
      361 gcatgaaaaa aatgacgaaa gttttgctgg ttgtactgtt ggcattggga ctttcaacga
      421 ctgcgtttgc tggcgcgtcc ggtctttcca tcgagcaaca cgctgacttt gacagggaga
      481 ttttcaccac cacctttacc ggctggcaag ccgactcaac cggcggatgg ttcttcttcc
      541 tcgatatcga ttccgcagcc gggtacaagc tcgggcacac cttcaattat tttgaaatca
      601 atcgagactt cagctttcgc aagctgttcg gcgacgaagg tctcaaccca ctcaacctgc
      661 atctcgaata caatgcctca acacatcttg ataccagcta ttttctgact ggtctcaaat
      721 acgagattag cggatatccc tacatcttca cgatttcggc ttcagcgaaa ctccccaaaa
      781 actcgaaagg tgaagagccg gatgcttctt ggcaggctac cgctgtctgg agtctcaatt
      841 ttgccgacgg cgcatttgtc ttcacaggct ttgtcgatgt ctggaacaat accgaaatac
      901 ccggcggcaa actggccttt atctccgagc cacaggtgct cgtgaatctc ggcaaactcg
      961 gccttggcaa agcaatggaa agcttctgga tcggaaccga attcgagatc agcaaaaact
     1021 tcgtctggtg ggatggagac gacatatttg tcaagcccac cgtctttatc cgctacgact
     1081 tctgatcctg atgcattgtc tccaacagcc cctctcgggg ctgttttttt attttcgcat
     1141 tccatcccgg gtcggtcttt tgccatatac accatgcccc gcactgtcta tatttgaggg
     1201 aattcagaga accaaggaaa aatccatgac acttgaactt gacaaaaagc aatacgagaa
     1261 tctcctgaaa ctggtctccc tcgggaacgc catggcaaac agctatcgcg aggaaacgat
     1321 cgaagagttc cagggaatct tcgatctcct ctacagcaaa gccgaagaag ccggcttggg
     1381 cgagatcgtc gaaaaggacg aggaaggatc cttctttccc accgaagatt tcgagctgat
     1441 gctcaattcc tttgtcgagg aatatgacga gtcctgcttt tgggacaggc tgatcgacac
     1501 ccttgtcgat cgggatcttg tcgcaaaatt cggtgaagaa gggtttgaaa aactctccga
     1561 agaggagctc gagaccgagc aggagaagct gatcaaattc tacgcgaccg aatttgaggc
     1621 aaacgacgtc gagaacctct tcctcaaaaa atgacgtgac cgtaccatca gggcatccgt
     1681 tcccggctct gccctgactg tcatcgtctc ctccgctttg aaaacatgcg ccgcagggcc
     1741 tttttcatcc cccgaataaa aagccagagc cctcccgcca cacgcgtatc gtgcaggtag
     1801 tagcgctgat gcacgtctcc catctccctc ccgtgctgca cgcccagaga acgggtcttc
     1861 ttgatgacaa gaacctgtgc agggtagata cttcggttgc cggtcatcaa aaaactgatc
     1921 aggcaggtga ctgcggcata gggtgcgatt tcgggcccga acagctccat ggccatgatg
     1981 ctggccgaaa gaggtgtatt ggctgccccg gccagaagcg agaccatgcc cagtgccgga
     2041 aagaccgggt tctggaattt caaaaggtcg gccagataca atccgcccag atgcgtgctg
     2101 gagatcgtgt agaactttgc gaagaaggat ccggacgccg caccgatgaa aaggacaggt
     2161 gtgaccaggc cggcaacacc tccggcctgg agcgtcaggg cggtaaacac cagtttgagc
     2221 agaaaatcaa accactcggc aggctgaccg ctcgtataga gcgccatctg cgcaaccccc
     2281 agcccgagat acttctccga ggtcagctcg accagaagaa cgatcaagac ccctccagcc
     2341 agggcccgga tcacaagacg cctggcatgc ttttcgagaa aacgccgggt agcacgcagt
     2401 gtctcgatca caagaaccgt caccaggcca aaaaagatcc ccgcaacaac caccttgagg
     2461 aaaaaatcct ccgtaaacaa cggaacaaat tttattggcg tatgcacata gactacaccg
     2521 atggcggacg ccacctgata cccgatgata ccggcaacga aggacgggaa gaggacttcg
     2581 tacagcaatg cccccacaaa aaggacttcg acgccaaaga tcgctcccga aatcggtgta
     2641 ccgaagaccg cagcgaaccc ggcactgatc ccgcagatca tcaacttgcg ccgatccgcc
     2701 ggatcaagcc tgaggagtct cgagagtccc gcactcaatc ctgccccgat gtcggcaccg
     2761 ggggcctccc gtccggccga gccaccacag gcgatggtaa tgatcggaag gaaaaacgcc
     2821 ttgacgacgg aaaaggggtg caccaagaca ttcttgtgga tactggcgat cacccggttg
     2881 gttgagtagc cgtcagccag cggaaccacc cgacgggaaa cccaggatgt acagaccagc
     2941 gccaggggaa gaaggtaata gtagttcgga attacctgaa cagatccgat ggcagcatcc
     3001 aaggccaaca tgaaacagcc gacgaccaaa ccgacactgg caccggaaac catggcgagg
     3061 aaaaaccact tgaggatact gaaaaacagg atcgattctt cggcaacctg gcgacggatc
     3121 acgaaccctc ctcctggatc atccttcggg gacagcgtgc atacatccag tccgcgatat
     3181 cgggcaagac caggtcaaca ccatccatgc agacatgatc cgacccgggc agatagtaca
     3241 tggccacatc actgcctccg atatcagtat acagccggat accgtccgat gccggaatga
     3301 tccgttcacg cccgcccacg acaaccaatg acggcacgct gatcgcaccg atcgtggttt
     3361 caagggaata cagccgaagc accgcccgta tcctccccga agatcgcaca cccagggcga
     3421 agcgaaccat ttcgtgaatg gcaaagacca ggcgcccata gtaccgcgcc gggcaatacg
     3481 ggcccgagat cgagacaatc cccagcaggc cgtcggcacg tccgcgatgg acgccaacct
     3541 gaaggacctt ccatccgccc agcgatgcac caagccagaa gctctccccc gtgacatacc
     3601 tggacgctgc ccggaccaga ctctcccgaa ccgcatcgta cagggcaccg ccatccggct
     3661 ccagtggcag catccgggcc gtttcaccga ccccgggatc gttcatcacc agaacggcga
     3721 atccccgtac gagaaagtgt cgcgcatact gcaggtattc ttccttccct gaatcagcgc
     3781 ctcccggtat gacaacggtt gtcctgagac cgtccctgac tgaccggggt ggcagaagaa
     3841 gccaaccgtt gaacgatgtt cccttccagg gaatccggac caagagggca tcaccgatcc
     3901 ccagagacac cccggcagac cgttcatgaa cgagctgcat cgcccgggtc attgcggtgt
     3961 ccgcccgttc catattgcgc aaccggatct ccccgcttgt acagagtgtc gaggaagctc
     4021 catgataata cagggccgcg gccagttcat gcccacaagc cgtttcgtac caacctgact
     4081 ggcaggcatc cctggcgagc gccaccctgc ctgccgcgag atttgcccaa gcacggctcc
     4141 accctccgat cgaacggact tttccgagaa cgtcctcgac ctcgaccagg accccgggcg
     4201 agcgaaaata gcgcacaaga taccggcgag gtatgatccg cgccgaacag aacaacagta
     4261 aaatcctgag aacaattttt cgggccagga ttttccgaaa aacgaaccca gccctctcgc
     4321 gccatcggct gatacgcggc agggcctgtc catccccccg gacaaagcgt tggacatgac
     4381 agtccgattc cggttgatgc atgcttttgt cgattttcat atctgtctac tcacgcttga
     4441 agagatcctt gatctggtca agtgtcagcc gcgagagatt gacggggctg tctccgatca
     4501 cggcctggac caaccgcttc ttgtcctgtt ggagcgccag aatcttctct tccacagttc
     4561 cttcggcgat caatttgtag accatgaccg gtctcgtctg tcccattcgc caggcacggg
     4621 ctgaggcctg gtcctcggca gccggattcc accaggggtc aacatggatc accgtatcgg
     4681 ccgttgtcag gttgagtccg gttcctcccg ccttgagact gatcaaaaag gcattcatcc
     4741 cggaatcttc attgaaacgc ttgacgatct cgcccctgtg cttgacattg gtcgaaccgt
     4801 ccaggcggca gaccgaaaac ccttcggcac ccaaccgctg ctcgatcagg tcgagcattc
     4861 cggtaaactg actgaagatc agtgtcttgt gtcccccgtc cacagcctgg ttcatgattt
     4921 cggagaggag ctccatcttg cccgaagtcg cccaaccgta tttctcgagg gccaggccgg
     4981 gatggcagca aatctgcctg aggcgggtca aagccgccag gagttcaagc gaatgctttt
     5041 ggacaccgtc cttttcgatc gccggcagga gactggaatc aaaaaacgaa aggaccgaga
     5101 gataggcttc cttctgctcg tcgaacatcg gacagatcga aacctgctcg gtcttgggcg
     5161 gaagctgggt cagcacctcg tccttggtcc ggcggagcat gaacggcgca atccgggact
     5221 tgagcgtggc aagcgcttca tcggacgagc cttccatgat cggcttttcg atttcatcct
     5281 tgaacctctg gtaggtaaac aggtaccctg gaaggacaaa atcgaagagc gaccagagct
     5341 ccccgagaca attctcgatc ggtgttccgg tcagggcata gcgggaccgg gcacggatgg
     5401 ccttggcact gatcgttcgg atcgaacccg gattcttgat atgttgtgcc tcgtcgagaa
     5461 aaatacaggc aaattccctg ttttcgagaa actcctggtc tttctggaga agattgtacg
     5521 aaatcacgac gacatggaac atgaagagtt ccttgtacat ctcctgcctc gccagtggtg
     5581 tccctccgac ggagagcacg gccagcgccg gcgcaaatcg cgagagctcg gccacccaat
     5641 tgaaaacgag ggacgaggga caaacaatca ggaagggatc cttttctccc gccgtgacgg
     5701 catactggat gaacgtaatc gtctggagcg tcttccccag tcccatatcg tccgccagaa
     5761 tcccgcccag actgtttgtg tagatgtttg ccagccagga gaggccgtca tgctggtact
     5821 cacggagggt ggcattgatc ccctcgggaa tggtgacccg ctttttccag ctcacaaggc
     5881 tctcggagaa gccctccagg gaggcatctc cttcgatatc accgtcctct tttggggcat
     5941 tctccctcat cgtttcggcc actaccggtg cgaaaatgct ctccgctgtc aggatgttcc
     6001 ggccatccct ccgcgccgga gaaaaatcca cccgccccgc caactctctg aggagggcct
     6061 tctgttcatc agaaagagga aggaactggc cattggagag ccggacatag ccctttccgg
     6121 cccgggccga ttcgaggacg gccatcggat ccagggccag tccccgttcc ctcggaaata
     6181 tggagagttc caaaaaatca tatttttttg acagcttgac accgaccctg ggacgcgaga
     6241 ggcgtcgggc cgcctgtttg aaggccgcat tgtaatagac atcccattgt ccggcatgct
     6301 ccggaaggac gaaatagaca aactcgaaga atttttgctc gtcctggagg acaaagtgcc
     6361 cgtcatcgtc ggccctgaac cctgctgcta caatcaggtc aacgatgtgt tgttcgtcct
     6421 gctgcctgcg gatctgccaa ctgtattccc cgctctggac caggcgaccg ggatgattcc
     6481 aggcgacccg ttcccggccg tagcggaatc cgagatcaag atcaagccgg atcaggagct
     6541 catgcatatc gacacggaca acgggtctgg gcggccggtt tgcctgcttg atgcatttgg
     6601 ccatgcggcc ttccattgtc agccgccccc gccgctccaa aaatggcagg agttcctcaa
     6661 gcaccgttgt cgcctcgaca cgttgaaccg tgatccgctc gggcatcccg gtatactgat
     6721 ggagggcatt ctgcatcgaa agagccagga gggtatccac ccggaccaga cgatcgccag
     6781 ccacggccca gaccggctgc aggggaagga gggagatggc gctactgctg cagtggaatt
     6841 cgccgttatg cagaacaaac ccgatctcca gatcccctcc cgagaggtca cggaccctgt
     6901 actcgcagtt gagggcatct ccatcgacaa agtattcttt tacgtcatca ccgccggcac
     6961 ggacaccaac ccgggttccg gccaaggcat ccagtacccc cggcagggcc gccgccgtga
     7021 gttgcaaccc ccgctggacg ggcagcgccg ctccggcatc ctgaagttgc cagagacagc
     7081 ccaagagccg ttcctccccg gcatcaagca gccagatgga aggatcgtag agaaccgcat
     7141 cggtaatccg catctgggag cgcgccttcc aggcatcaag gaaggcaccc ggatcgggaa
     7201 cgaggagtcc atcggatgcc tggatccgca actggacggt gcagctccgc tccttgcgga
     7261 agaggccacg gagatccagg atggcatcca gcccgggtga gaccgagccc gagggtggaa
     7321 caaaatgcat gctctccctg aagaactcga gaaccccccg ggctttcccc ggtgaaccca
     7381 cggcagacgc cctggccggg agccgctttt tcagggcata gagggctgca atcacatggg
     7441 cacagcggcc ggtcgcctgg ccgcaggtac acgtcccctc aaggcatgtc ccctgttcct
     7501 cgagacgcac atcatggaga cgctcatcgg ccaccctgca ggaaaacacc ccgggtccgc
     7561 tttcaaacga gagaaccgaa ccctcatgca aaagccgttt tccgttccgc acatcttcgt
     7621 ccgaagcacc ggcctcgata cccgcctgcg tcaagagatg acgtacccgt tcaagatgct
     7681 gcgttgtcat gaccctgccc gactctgtcc tctgaatgcg ccccgatacc ggcatccgca
     7741 gcattgcgca taatccgaag cggatgcatc cacgttgaat ttacaaaaag gccgtgcctg
     7801 tgtaccagaa aagaccgctc cccggaaccg atccccgggg gaggcaagaa attatgacag
     7861 gtttctgtcc gtgtggagtc cagtccccaa tcggcttttc ccggggtgcg cacaaaaacc
     7921 agccacgccc cggacctgat ccggcacaag agaacctctg gaaacagcag catgtggttt
     7981 tgtaaccggt cggggcaatc ccgcccggaa tcaatcatcc cattcgttga aaaaaatcgc
     8041 aaggatcagt tcgacttccc cccccgagag ttttatccct tccttgcgca aagcatcccg
     8101 aagggcgtct gcatcaaatt tgggattggc cgtcttgatg gcaacagccc gtttgacgga
     8161 tttttcagga atctcgtcag gtccgcagag gaacccggta ttgaagagcc ggagcgtggc
     8221 gtgtcgcaaa agctggtgct cccgctcaag acgggcattt cgtgccacca actcctctag
     8281 gagcctgtcc agacgatcaa gacgcgccat ataattggtc ttgtccgcaa aggtcaaagg
     8341 gttatcgatc gcccgctgga tgagtgttgc cagtccaacc cggtcaatga aacgcccggc
     8401 aatcctcatc gagattgaat ccttgaccat ccggaaatcg aactggacat actcccgata
     8461 gtagaaaccg atcccggccg gcatgaaacg ggcaacatcg gtcccctcta tcgtgagccg
     8521 ctcggggaca accaccgcct caaccgtctg tcctgagagg atgatcgaga gggtgcgtac
     8581 ctcgaggccg gcccaagagt ggacaatatc ccgcagctcg gcgatcttgc gcagattggc
     8641 agcatccggt gcactctccc aggaaatcga acactctctc ccggaagcat ccttgagact
     8701 ggccacaacg cggccatcct cctgattcgt ctgggaaacc agtgtcaatc cgattgtcga
     8761 aaaattcgga atgtcctcgg ccgaaaaagc cgttccgccg atacagagca ttcccgcgac
     8821 aagcacaatc atcagacagg atttcatctc attctccatt tccgggcaca cacctgcggg
     8881 tcatcgcagc tgcagcagta atttgtcaag agccgccggc ttgtcagaaa acgcaccctc
     8941 gggaatggca atcagtccat cccgggagcg ttcaagccgg accatcccga tcctcgtatt
     9001 ctcgccacga atcacgaacg ccatatcccc gcttttaacc tgataggctg ggttgacgat
     9061 gaccggatag gcatccggac ggagcgtatc gaggatatac ccatgctccc gcgtctgttg
     9121 catcagggcg gcaaacgccc tgttgtaaga atccaggcgc gagtttttct gttcgatccg
     9181 ggctgccagc ccgctccaga gtgcttcata tcctgcaacc acggccgcct gcctgttgcc
     9241 caaggcattc aaggccggcg ttatcgaatt ggtataccgg tttgcaatga tcctctcgag
     9301 gatcgttctc tcatcccgga tgttcttccg cagggaagca aatccggcct gatccagcac
     9361 accttcacgg gcaaggatcg cctgccacgc atgttgtgtc cctggtccgg agagccgcga
     9421 gttgccggca atcaacccct gcatcaaggc atccccgaat accgggttat agcgtaaaac
     9481 gagggaatcc gtcaattttt tttgcaggcg gcgctgttct gccgcttcga ggcggaaacg
     9541 gttctgggcc tcgtccagcc gctgccgcca ctgatcctcg atttttttgc gctcgatctc
     9601 gatcaatttc aattgactcc cgaggaccat ttccatggcc tgggcaccct ggaccatttt
     9661 tttgtcccga ccggcaagac gcttcgtgat ctcgtcgatc tccttctgtc gctcggcaat
     9721 ggccttctgg tagcgggcat ccagtgtacc catctcagcc ctttcattcg cctggaccac
     9781 ggcaagcgca cgctgcctgt ctgcctcatc cgcgtaccgg atggcggcaa tatcccgttc
     9841 ggtgatatag cgatcccgga ccttctgcgc ctccatctgc cagttacccc tgaaatcggc
     9901 aatctcctgc atcaggctcc gcaggcgggt ctcgtccttt ttggtattct gcaaaagatc
     9961 cgtcagattg aggtccctga aatccgaaac atccaccttg atggcatcgc tctgccgccg
    10021 gacttccccg gtaatcacaa gggtcaccac gaccaaggct cccgcaaaca gaaccgcacc
    10081 ggcatacaca aagagatgct tgcgcttttt ggtcttggca aactcgtcct cgagagagta
    10141 aaggacggga cggtccatcg tctcgaccgc attctcgcgg aaaaagactt cagtctttct
    10201 ggtcagaccc cggcttactt tgttgtcatt tcccatatac cgctccagtt tcgcgggcag
    10261 gtgccctggg tccgtgttgc aaaaatcgcg gccggctcga aggaacagga tttgaacagg
    10321 gcattggccc gcttccagtt tcctttgtag tacagttcga gagcttcttc atactgtgca
    10381 aaggcggtct tcatcgagcg gttcacattc gctgcttcga ccggataata gatccggcgg
    10441 cc
//
LOCUS       DAOSHE010000017        10204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5591_length_10204_cov_4.280323, whole genome shotgun sequence.
ACCESSION   DAOSHE010000017 DAOSHE010000000
VERSION     DAOSHE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_10204_cov_4.280323"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLD82_01045"
     CDS             complement(<1..803)
                     /locus_tag="PLD82_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG64006.1"
                     /translation="MMDWFFVGMACLLAGAFLVPFVPAQRRPVAFTLVILLSAIAETL
                     ALAPVFSGGGTVSSIVPFGWPLGDALLRIDRLSAFFLLVIAWLGLPALVVSGRFTRMD
                     TVDGARIHWFFLGMLFLAMKLAIVLESLIHFLVVWEIMSLSSFFLVLMGNSLRRIRSA
                     GLHYFVAMHISVVFLMLGAVVLGGGGFTNLHGTTGLSFLFLMTGFAFKMGLVPFHSWI
                     PDVYEGAPTPVAAFFASAPKVAAMGLFLRVALDAFSGMIADWRQLVIFMA"
     gene            complement(800..1480)
                     /locus_tag="PLD82_01050"
     CDS             complement(800..1480)
                     /locus_tag="PLD82_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG64007.1"
                     /translation="MHLSTADVAAMLGLREQDVLSRVSTGDLPGRKIGAEYHFLKTEI
                     DEYILRQSIVVTPAYFTAGDSAPFLLAGLLKRGGLVMNIPGTTMAEVIQHGVHTLPAF
                     PGLDREDCIRALLSRESQLSTAIGGGIAIPHPRSPIIADCDQESITVLRPAQPVVAIS
                     PDGLPVHTMFLVLSSNARRHLEILARLAWVLSDGGMRRAVQTAASLDELVRIVSTLDQ
                     AISGGKVP"
     gene            1562..>1847
                     /locus_tag="PLD82_01055"
     CDS             1562..>1847
                     /locus_tag="PLD82_01055"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOG64008.1"
                     /translation="MNVFSLAAQVASVVYLYTGAFSLWLDRSNPLNKAYFGLSLCFAY
                     WAMTYAQVYSAPDAANAWFWYDLSSFGRVTWYAFFLVFALILTDRASLFKK"
     assembly_gap    1848..1857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..4555
                     /locus_tag="PLD82_01060"
     CDS             <1858..4555
                     /locus_tag="PLD82_01060"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64009.1"
                     /translation="LPLIFLPPTLFITQNLTGYLGTSNLVSGPLGFIEIPARNALYYL
                     YVGTYAGYTVFGLHLIYIWTRRQTAARKRSQGFMIFVSGYIAFVVSLLTNLFFPLLGV
                     HTVPALAPILILFWSLGIFYTIRKHHFMLVTPAIVADEIILRMRDMLLLTDSEMRVVR
                     INPETERLTGRTAVDLIGKPLTSILSAHALNCPEHVEHETTVTTSLGEEIPVRLYRDI
                     IHDRSGEPIGTLIMASDIREQLQLLQEVQDRMRIEGELVHAKEAAEEANHMKGRFLAN
                     MSHEIRTPMNGVCGMAELLLDTRLDPEQQEYARTIISSGKALLGIINDILDYSKIHEG
                     KLEIENTPFHLGRLANELILLLYPRAREKEIELVLHYSPQARMDVIGDQTRIRQVLLN
                     LLNNAIKFTEKGCIILSIEIVRCSPDGGLFTITVEDSGIGIPPEKTEAIFERFSQADA
                     STTRMYGGTGLGLTISRQLVELMGGTLTVTSFPETGSTFWVSLPLPWQQQGACEINSL
                     PCKTLLFDKRHIPGSMLQKIVADCGSDCLLVNAWSSVRERPDVCLVLVHESMPDASGT
                     ETLAMLRTHFTNARLVIIHQPGLDVHGFDATITEPVRPSQIMSVLSAVVKTCHLHSPS
                     GGTFIIEPESYLSMNAPITCLVFPGKKILLVEDNEVNRTVALRLLKKLGCTVDCAENG
                     QLALDMLATREYDLIFMDCQMPVMDGYTATSMIRQPGSPWSRPEIPIVALTAHAGGEE
                     HAKAREHGMDSCLTKPVSRKNLETCLAEIFNPEMEVTAENTPVRGDQVLNEARAMERL
                     DGDRTLWLELVQIFLESLPDAKETLTRAILGDDRPGIAAIAHTIKGGALNCSADEIAN
                     IFAVLEDSCLTLSSGELNDALVSTDLAFARLLQEAFALRNRN"
     gene            complement(4583..5389)
                     /gene="grpE"
                     /locus_tag="PLD82_01065"
     CDS             complement(4583..5389)
                     /gene="grpE"
                     /locus_tag="PLD82_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG64010.1"
                     /translation="MRGYGRILLCVLLMVLFEVVCVSRELSAISGEQVLLAQVPGRKP
                     VEKTSAGATQPDRETKAPETVQSPEISPDRILDTIIDVNTKYSYIAIAIAGLTSVLVL
                     LILVRMGGVRNSIGKIERDVMHIKEALRYLMESAQHLPPPEAKEKGSRPVPAPEPAGY
                     SAALLKVFAELELLSRKFDSFPRPATSGNKQLFSEDEVRREVERRLDEMRIETIVPAP
                     GDMFDAVLHEEVDIKPSRADDDGTIFRVIQNGLVYNSRVALKARVVVNRA"
     gene            5527..6519
                     /locus_tag="PLD82_01070"
     CDS             5527..6519
                     /locus_tag="PLD82_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG64011.1"
                     /translation="MTDEKNFWLALPTPFLCLAPMSNITDSAMRLILARHCKPDVIFT
                     EFISTAGLTSPKGRSKLLPDLRFDPGEHPLVAQFFGRVPSQFFACASLAADLGYDGVD
                     INLGCPDRSVLRQGAGSSLIREPALVAEIIQAAREGARGLPVSVKTRIGYSQNTVETW
                     IGQLIGMKPAAITLHGRTRKQKYLGSADWSAIAHAASMAREAGIPLIGNGDVASWQDA
                     MDRAREAGVDGVMIGRAAIGNPWVFSPETTRKKIPLEDLLSVIIEHAGLYQEHYDGIK
                     SFANVRKHLKNYVADFHGAKSLRIALVHAENADHVREIVHDFLHNPPASDSQQE"
     gene            complement(6671..7675)
                     /locus_tag="PLD82_01075"
     CDS             complement(6671..7675)
                     /locus_tag="PLD82_01075"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64012.1"
                     /translation="MAVTGEGSGKLLLFGEHAAVYGYPALGLALPWKTRVTLDENTSL
                     SAWALPELNGDESARLHALVSLMFDTFPLLRKDGYRLTIHSDVPRGMGFGSSAALCVA
                     LVKAVVTVLANRFLPEAWARELLRELGRLWALANKAERLFHGKPSGIDTGLALFGKMQ
                     GFSFSGTGDDLPQHTEMKTCPLTLIVGGVPRDGSTKAHVQAISERYRKKDQETKSAIA
                     HLGALSAEAIQCFHQAGSGSNDQRELAEKIGNLADQAETSFRQLGLGNGLVDDLLSCG
                     RQAGATGGKMSGAGGGGAFYLVSPDEERAARIFERVRARLDANGLADSPLMIIRKTDN
                     "
     gene            complement(7666..8667)
                     /locus_tag="PLD82_01080"
     CDS             complement(7666..8667)
                     /locus_tag="PLD82_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64013.1"
                     /translation="MTREYIVPGNLLLAGEYAITAEGGLGIALAPARFLRLRAVPGSR
                     FLLEGQAHGAALDGGRLAEAVLAAAAEILSVPVSDKRFAARVFADSTELFFAPGKKLG
                     LGSSAALAVGLAAFFVDCIHPLGPEPSPEECEPVLRIALRGHRAFQSGGSGYDVHASF
                     YGGTGLFRGGEKPLWNPIRIADGMELTLIPAEESVRSGPAVRSFKKLQAERGVDVARF
                     LACSNRAVRHLEAALTAGAGQAGDGQIRGPSACALFRAITGVRRVGLWIGRNLGLDPE
                     GGQLAGVLADLRTRGIPAKASGAGGELAIAFGFPPVYYSPEVEVFSVKLARDGVRWR"
     gene            8769..9494
                     /locus_tag="PLD82_01085"
     CDS             8769..9494
                     /locus_tag="PLD82_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HOG64014.1"
                     /translation="MAIASQSILVEEKQDGFLIKVDGTPLKTPAGATILLPTKRLAQA
                     MVAELVTVDEIDPSRLGFYCLASTRLDFTDKGKSIPVEGIPKIILFDPALHALPGSAL
                     DVLESATEPVRSFLAGHGLVHPEIPNLPENEMEGWLADSPSETKHNIEGILRVCRDLF
                     EKQGNAQHAVVINAGKVHRSFVLGLLLAEGQISTAEYAAAVMATLCIHPLAWNNIGVD
                     QYRAAFEEVRLQADSMRVYLEEN"
     gene            complement(9501..>10204)
                     /locus_tag="PLD82_01090"
     CDS             complement(9501..>10204)
                     /locus_tag="PLD82_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64015.1"
                     /translation="DYVWGYDPQDVVHWLGLTKKKEYISIGEYWQSSNDRSDDPLIQR
                     YGRDEGARIIGWAAETGCLAYDIVLKRQIQTAHPANLRWGLNMSPDRAVRESVMTFVD
                     NHDMGASPWSAANGWGQQCWPCPPEFKSKAYAFILTAPGIPCVYWPDCYDWGFEWMIS
                     ALIRLRQARGVTAGSSWHEVGGGYSGFAYAVSGREGKDALIVSLGSDYPGPGKGWKIA
                     LSQPGEWIVWEPDEG"
BASE COUNT         2302 a         3243 c         2748 g         1901 t
ORIGIN      
        1 gccatgaaga tcaccagctg ccgccagtcc gcgatcatgc ccgaaaaggc gtcgagcgcg
       61 acgcgcagga acaggcccat cgcggcgacc ttcggcgcag aggcgaagaa ggcggcaacc
      121 ggggtcggcg cgccttcata gacgtcgggg atccaggagt ggaaggggac cagccccatt
      181 ttgaacgcga acccggtcat caggaacagg aaggagagcc cggtcgtccc gtgcaggttc
      241 gtgaaccccc ctccaccgag gacgacggca cctagcatca ggaagacaac actgatgtgc
      301 atcgccacaa aatagtgcaa ccccgccgac cggatccgcc tgaggctgtt ccccataaga
      361 acgaggaaga aagacgagag cgacatgatc tcccagacaa ccagaaagtg gatcaggctc
      421 tccaagacga tggccaactt cattgcgaga aaaagcatcc ccaaaaaaaa ccagtggatc
      481 cgcgcaccgt ccacggtgtc catcctggta aatcgcccgg aaacaaccag cgccggaaga
      541 ccgagccagg cgatgaccag cagaaaaaag gccgagaggc ggtcgatccg gagcaaggca
      601 tccccgagag gccagccaaa gggaacgatg gatgaaactg tccccccacc ggagaagacc
      661 ggcgccaagg cgagggtttc ggcaatcgcc gagagaagga tcaccagggt aaacgccact
      721 ggtctccgct gcgccggcac aaagggaacc aaaaatgcgc ctgcaagaag acaggccata
      781 ccgacaaaga accaatccat catgggacct tcccgcctga aatggcttga tcaagcgtgc
      841 tcacaatcct gaccagttca tcgagcgatg cagccgtctg gacagcccgg cgcattccgc
      901 catcggaaag cacccaggcc agccgcgcca gaatctcgag atggcgccgg gcattggagg
      961 acaggacgag gaacatggtg tgcaccggca gtccgtccgg cgaaatcgcg accacgggtt
     1021 gcgcagggcg gaggaccgtg atgctctcct gatcgcaatc ggcgatgatc ggactccgcg
     1081 gatggggaat cgcgatccct ccaccgatcg ccgtggaaag ctgggactcg cgggagagga
     1141 gagcccggat acaatcctcc cgatcaagac cgggaaaggc cggcagggtg tgaactccat
     1201 gctggatgac ttcagccatg gttgtcccgg ggatattcat gaccagccca ccgcgcttga
     1261 gaagcccggc aagcaggaac ggggcactgt ccccggcggt aaaataggcc ggtgtcacga
     1321 caatcgattg acgaagaatg tattcgtcaa tctcggtttt gagaaaatgg tattccgcgc
     1381 cgatctttct ccccggcaaa tcgcccgtac tgactcggga aagaacatcc tgctcgcgca
     1441 ggccaagcat ggccgccaca tccgctgtcg ataaatgcat acctgaacca ccctctgcaa
     1501 gtatacgcag tcctcttctt cgctggattt tttttttgat cccgtgctat actgacacat
     1561 catgaacgta ttctccctgg cagcccaggt cgcaagcgtg gtttacttgt ataccggagc
     1621 cttctccctc tggctggacc ggtcgaatcc gctcaacaag gcgtacttcg gtctctctct
     1681 ctgtttcgcc tactgggcca tgacctacgc ccaagtctat tccgctccgg atgcggcaaa
     1741 cgcctggttc tggtacgacc tctcctcatt cggccgggtg acctggtacg cgttcttcct
     1801 ggtctttgcc ctgatcctca cggatcgggc atccctcttc aaaaaacnnn nnnnnnngct
     1861 ccccctgatc tttctccctc caaccctctt catcacgcag aacctgacgg gctatctcgg
     1921 taccagcaat ctcgtctccg ggcctctcgg ctttatcgag ataccggccc ggaatgccct
     1981 ctattatttg tatgtgggaa cctatgcggg ctacaccgtg ttcggcttgc acctgatcta
     2041 catctggacg agacggcaga ctgccgcccg gaaacgcagc cagggtttca tgatcttcgt
     2101 ctcgggctac atcgccttcg ttgtcagcct gctgacaaat ctctttttcc ccctcctcgg
     2161 cgtgcatacc gtcccggcac tcgctccgat cctgattctc ttctggtccc tcggaatctt
     2221 ctataccatc cgcaaacacc atttcatgct cgtcaccccg gccattgtgg ccgacgagat
     2281 catcctccga atgcgggaca tgcttctttt gacggacagc gaaatgcggg tcgtccgcat
     2341 caatcccgaa acggagcgac tgacgggacg aacagccgta gatctcatcg gaaaacccct
     2401 gacgagcatt ctctccgcgc acgctctgaa ctgcccggaa catgtcgaac acgagacaac
     2461 ggtcacgacc agtctggggg aagaaatccc cgtccgtctc tatcgcgaca tcatccatga
     2521 ccgatccggc gaaccgatcg gaaccctgat catggcaagt gacatccgcg agcagctcca
     2581 acttctgcag gaagtccagg acaggatgcg catcgagggg gaactggtcc atgcgaagga
     2641 agccgctgaa gaggccaacc acatgaaagg ccggttcctg gccaacatga gccacgagat
     2701 ccgcactccg atgaacggtg tctgcggaat ggccgagctc cttctggata ccaggctcga
     2761 tccagagcag caggaatacg cccgcacgat catcagctcg ggaaaggcac ttctcgggat
     2821 catcaacgat atcctcgact actcaaaaat ccacgaaggg aaactggaga tcgagaacac
     2881 ccccttccat cttggacgtc tcgcaaacga attgattctc ctcctctatc cgcgtgcccg
     2941 ggagaaagag atcgaactcg ttcttcatta ctccccgcag gcacgcatgg atgtcatcgg
     3001 tgaccagacc aggatccgcc aggttcttct gaacctgctc aacaacgcta tcaaatttac
     3061 cgaaaaggga tgcatcattc tctcgattga aatcgtccgt tgttctccgg acggtggact
     3121 ctttaccatc accgtcgaag acagcggaat cggaatcccg cccgaaaaaa ccgaagcgat
     3181 ctttgaacgc ttcagccagg cggatgcgtc cacgacgaga atgtatggcg gaaccgggtt
     3241 ggggctgacg atctcgcgac agttggtcga gctgatgggc gggaccctga cggtgaccag
     3301 cttccctgag acagggtcga ctttttgggt ttcactcccc ctcccctggc aacagcaggg
     3361 tgcctgcgaa atcaactccc tgccctgcaa gaccctcctc ttcgacaagc gccatattcc
     3421 aggaagcatg ctgcaaaaaa tcgtggcaga ctgcggaagt gactgcctgc tcgtcaatgc
     3481 ctggagttcc gtccgggaac ggccggacgt ctgcctcgtt ctggtccacg agagcatgcc
     3541 cgatgcaagc ggaacggaaa cactcgccat gttgcggact cacttcacga acgcccggct
     3601 ggtcatcatc catcaaccgg gattggatgt ccatgggttt gatgccacca ttacggaacc
     3661 tgtccggcct tcacagatca tgtccgtcct ctccgctgtc gtaaaaacat gccaccttca
     3721 ttcaccatcg gggggtacgt tcatcatcga acccgagtct tacctctcca tgaacgcccc
     3781 gattacctgt ctcgtcttcc cgggaaaaaa gattctcctc gtcgaagaca atgaagtcaa
     3841 ccggaccgtc gccctccgtc tcctcaaaaa actcgggtgt accgtcgact gtgccgaaaa
     3901 cgggcaactg gccctggaca tgctcgcaac ccgggagtat gacctgatct tcatggactg
     3961 ccaaatgccg gtgatggacg gctataccgc cacatccatg atccgccagc ctggctcgcc
     4021 ttggagcagg ccggagatcc cgatcgtggc actgacggca catgccggag gggaagagca
     4081 tgccaaggcc cgcgaacacg gtatggattc ctgcctgaca aaaccggtct cgcggaaaaa
     4141 tcttgaaacc tgtctggcgg agatcttcaa tccggaaatg gaagtgacag cggagaacac
     4201 acctgtccgg ggggatcaag tcctcaacga agcccgggcc atggagcgac tggatggaga
     4261 caggacactc tggcttgagc tggttcagat ttttctcgag tcattgccgg atgcaaaaga
     4321 aacgctcacc cgggccatac tcggagatga ccggccaggg atcgccgcga tcgcccatac
     4381 catcaagggc ggcgccctca actgcagtgc cgacgagatc gcgaacatct tcgctgttct
     4441 cgaagactcc tgcctgactc tttcttccgg ggaactcaat gacgccctgg tttcgactga
     4501 cctggccttt gcacgactcc tgcaggaagc cttcgcattg cgcaacagaa actgacccgg
     4561 taaatctccg ggggagcgtg gatcaggccc ggttgacgac cacccgcgcc ttgagcgcaa
     4621 cccggctgtt gtagaccagg ccattctgga tcaccctgaa gatcgtcccg tcatcgtccg
     4681 ccctgctcgg tttgatatcc acttcctcat ggaggaccgc atcgaacatg tcgcccggcg
     4741 caggaacgat ggtctcgatc cgcatctcgt caagccttcg ctcgacctcc cggcggacct
     4801 catcctcact gaagagctgt ttgttgcccg acgtcgcagg ccggggaaag gaatcgaatt
     4861 ttctggaaag aagttccagc tcggcaaaga ctttgaggag ggccgcgctg tatcctgcgg
     4921 gctcgggagc aggcaccggc cgggaaccct tttccttcgc ctccggggga ggaagatgct
     4981 gcgcactctc catcagatag cgcagggcct ccttgatatg catcacatcc cgctcgatct
     5041 ttccgatgga attccggaca cctcccatcc tgaccaggat caggaggaca agcacagagg
     5101 tcagtccggc gatcgcaatc gcaatgtacg aatatttcgt attcacatcg atgatggtat
     5161 ccagaatccg gtccggactg atctcgggag actgaacggt ttccggagcc tttgtctccc
     5221 gatcaggctg cgttgccccg gcagatgtct tctcgaccgg tttccgcccc ggcacctggg
     5281 caagcaagac ctgctccccg gaaatggccg acaattcccg gctcacgcag actacttcaa
     5341 aaagaaccat caacaaaacg cacagcaaga ttctgccgta tccacgcatc acgcacgccc
     5401 tccagacaca ggagaatata gacggtttgt gacatcttgt gcaatgagaa attgaaaccg
     5461 ggacagttcc cctgattttc tgtagatttg gcggaagccg gacggaagga ttggcaggtt
     5521 ggacgaatga cggacgaaaa aaatttctgg ctggccctcc ccaccccctt tctctgtctc
     5581 gcccccatgt ccaacatcac cgacagcgcc atgcggctga ttctggcgcg tcactgcaaa
     5641 ccggatgtca tttttaccga gttcatctcg accgccggac tgacatcgcc caagggccgg
     5701 agcaaactcc tccccgatct ccgtttcgat ccgggcgaac acccgctggt cgcccagttt
     5761 ttcggacgtg ttccgtccca attcttcgcc tgcgcatccc tggccgccga tctcgggtat
     5821 gacggggtgg acatcaacct cgggtgcccc gaccgctccg tcttgcgaca aggtgcgggc
     5881 tcgagcctga tccgggaacc ggccctggtg gcagaaatca tccaggccgc acgggaaggt
     5941 gcccgcgggc tcccggtttc cgtaaaaaca cgtatcggat attcacaaaa caccgtcgag
     6001 acctggatcg ggcaattgat cggaatgaaa ccggccgcca tcaccctcca cggacggaca
     6061 cggaagcaaa aatacctcgg ctcggccgac tggtcggcca tcgcccatgc cgccagcatg
     6121 gcccgcgagg ccgggattcc cctgatcgga aacggggatg tcgcatcctg gcaggacgcc
     6181 atggaccggg cccgcgaggc cggggttgac ggcgtgatga tcggcagggc agccatcgga
     6241 aatccctggg tcttttcacc cgaaaccacc cgaaaaaaaa tcccgctcga ggatctcctc
     6301 tccgtcatca tcgaacatgc cgggctctac caggagcatt atgacgggat caagagcttc
     6361 gccaatgtca ggaagcatct caaaaattac gtggccgatt ttcatggagc caaatccttg
     6421 cggatcgccc tggtccatgc cgagaatgcc gaccacgtca gggagattgt ccacgatttt
     6481 ctgcacaatc ccccagccag cgattcgcag caggaatgaa cccgcgccag gccggacgag
     6541 gcatgacttc ctgcaggttc tttttttggg gaacaacctt ccatcgccca atgggctgtc
     6601 gcctgcctcg aacgggattc accccgctgc tccccgacac ggatcggccc ggcaacgaaa
     6661 ggggaatatt tcagttatcg gtctttctga tgatcatcag gggactgtct gccagaccat
     6721 tcgcatccag ccgggcacgg acacgctcaa aaatccgggc agcccgctct tcatcgggag
     6781 agaccaggta aaaagcgcca ccaccaccgg cgccactcat tttccctccg gtcgcacccg
     6841 cctggcgtcc gcaggacaaa agatcgtcca caagcccgtt ccccaatcca agctgacgga
     6901 atgaggtttc ggcctgatcg gcaagattgc cgattttttc agcaagctca cgctgatcgt
     6961 tcgaacctga tccggcttga tgaaagcatt ggatggcctc ggcggagagt gcgccaagat
     7021 gggcgatcgc actcttggtc tcctgatcct ttttccggta tctctcggaa atcgcctgga
     7081 catgggcctt ggtgcttccg tcccggggaa caccaccgac aatcagggtc aagggacagg
     7141 tcttcatctc ggtatgctgc ggaagatcgt ccccggtgcc ggaaaacgaa aacccctgca
     7201 tcttgccgaa cagggccagc cccgtgtcga taccggaggg cttcccgtga aagagccgct
     7261 ccgccttgtt ggccagagcc cagagcctgc caagctcccg caggagctcg cgcgcccagg
     7321 cctcggggag aaagcggttg gcaagaaccg tcacgactgc cttgacaagg gcaacacaga
     7381 gggcggccga tgagccaaaa cccatacccc tgggcacatc ggagtggatc gtcagacggt
     7441 acccgtcctt cctgagcagg ggaaatgtgt caaacatcag actgaccaag gcatggagac
     7501 gcgccgattc gtcgccgttg agttccggca gggcccatgc cgagagagag gtgttctcgt
     7561 caagcgtcac ccgggtcttc cagggaaggg ccagtcccag ggccggatag ccgtagaccg
     7621 ctgcatgctc gccgaagagc aggagtttcc ccgagccctc gccagttacc gccatcgcac
     7681 accatcccga gccaatttca cgctgaagac ttccacttcc ggagaataat agacaggagg
     7741 aaatccaaaa gcaatggcca actcgccacc ggccccgctc gccttggcgg gaatcccccg
     7801 ggtccggaga tcggcaagga cccccgccaa ctgcccgcct tcggggtcca gtccgagatt
     7861 ccggccgatc cagagaccga cccggcgcac gcctgtaatg gcgcggaaaa gagcgcacgc
     7921 ggaaggcccc cggatctgcc cgtcgcccgc ctggcccgcg ccagccgtga gggccgcttc
     7981 gagatggcgc accgcccggt tggaacaggc caaaaatcgc gcgacgtcca ctccccgttc
     8041 ggcctgcaat tttttgaacg atcgcaccgc gggcccggaa cgcacgctct cctccgcagg
     8101 aatcagcgtc agttccatcc cgtcggcaat cctgatcgga ttccacaatg gtttctcccc
     8161 accacgaaac agtcctgttc ccccataaaa agaagcatgg acgtcatacc cgcttccgcc
     8221 gctctgaaag gccctgtgtc cccggagtgc gatccgcaag accggttcac attcttcagg
     8281 cgaaggctcg ggaccgagtg gatggatgca gtccacaaaa aatgcggcca acccaaccgc
     8341 gagagcagca ctcgaaccga ggccgagttt tttccccggt gcaaaaaaca gttcggtcga
     8401 atcggcgaaa acccgggcgg caaaccgttt gtccgagaca ggaacggaga gaatctccgc
     8461 tgccgcggcg aggaccgcct ccgcaagcct cccgccatcg agcgcagccc catgggcctg
     8521 accttcaagg agaaaccggc tcccgggaac agcacgcaat cgcagaaaac gggccggagc
     8581 gagcgcaatc ccgaggccac cctccgctgt gatggcatac tcgcccgcca gaaggagatt
     8641 cccgggaacg atgtattctc gtgtcatccc acaaatctac gccgtttttc cggttcatga
     8701 tcagcaaatc ctgccggtct gagcggcagt gagtatcttg gatggaacac ggaaagcgga
     8761 gaagacccat ggccatcgca tcacaatcga ttctggttga agaaaaacag gatggattcc
     8821 tgatcaaagt cgacggaacc ccgctcaaga ctccggccgg ggccacgatc cttctcccga
     8881 caaagcgact ggcccaggcc atggttgccg aattggtgac ggtcgacgag attgatccct
     8941 cgcgtctcgg attctactgt ctggcctcga cccgtctgga ttttacggac aagggaaaga
     9001 gcatccccgt tgaaggaatc ccgaagatca tcctctttga tcccgccctg cacgccctgc
     9061 ccgggagcgc cctcgacgtc ctcgaatccg ccaccgaacc ggtccgcagc tttctcgccg
     9121 ggcatggtct ggtccatccg gaaataccga atctgcccga aaacgagatg gaaggctggc
     9181 tggccgactc tccgtccgag accaagcaca acatcgaggg gattctcagg gtctgtcgcg
     9241 acctgtttga aaaacaggga aatgcccagc atgccgtggt gatcaacgcg ggcaaggtcc
     9301 atcgttcctt tgtcctcggg ctgctcctcg ctgagggcca gatctcaacc gccgaatacg
     9361 cggcggccgt catggccacc ctctgtattc atccactggc atggaacaac atcggggtcg
     9421 atcaataccg ggcggccttc gaagaggtca ggctccaggc cgacagcatg cgagtctacc
     9481 ttgaagaaaa ctgatgctcc tcagccctcg tcgggctccc agacgatcca ttctccaggc
     9541 tgagaaaggg caatcttcca tcccttgccc gggccgggat aatcggaccc gagggaaaca
     9601 atcaacgcat cctttccctc ccgaccagac acggcatagg caaatccact gtacccgcca
     9661 ccgacctcat gccaggagga tccggccgta actcctctcg cttgccgaag ccgaatcaga
     9721 gcgctaatca tccactcaaa tccccagtca taacaatcag gccaatagac gcacggaatt
     9781 cccggtgccg tcaggataaa agcgtaggcc ttcgatttga actcgggagg gcatggccaa
     9841 cattgctggc cccaaccatt ggccgccgac catggagaag cccccatatc atgattatcc
     9901 acaaaggtca tgacactctc acgaacagca cgatccggag acatattcag accccagcgc
     9961 aggttggccg gatgcgctgt ctgaatctgg cgtttgagaa cgatatcgta ggccaggcat
    10021 ccggtctcgg ccgcccatcc gatgatcctg gcaccttcgt cccgaccata gcgctggatc
    10081 aggggatcat cactccggtc attggaactc tgccaatact caccgatgga gatgtattcc
    10141 ttttttttgg tcagcccgag ccaatgcacc acatcctggg gatcatagcc ccagacataa
    10201 tccc
//
LOCUS       DAOSHE010000018        10204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5592_length_10204_cov_4.054685, whole genome shotgun sequence.
ACCESSION   DAOSHE010000018 DAOSHE010000000
VERSION     DAOSHE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5592_length_10204_cov_4.054685"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLD82_01095"
     CDS             <1..623
                     /locus_tag="PLD82_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG64016.1"
                     /translation="FRGTIDMTDVVAIGRAVKGMPLLQLKPAISGRNGMTVRGEEIKA
                     ADVKSLQIETRRNTKRAGDLIVPELDGIVYLYDSAILVKAYADAEVQIELDDEKMNAF
                     VNLIPSVGDGLKLDMGIAREALTKAGITNGVNERMLEDAITKCEADKMPFPASPSPGE
                     PSRSTARTAKSCSRSACRAPTNRSRKMAAVSITAKKIRSSTSTRIP"
     gene            515..>1233
                     /locus_tag="PLD82_01100"
     CDS             515..>1233
                     /locus_tag="PLD82_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG64017.1"
                     /translation="MFKIRMQGSNEPVEKDGGRVDYREKDSIINIDENTLIAVQFPAQ
                     KKISDGMTVTGETIPGTDGNPVDIKLGKNIDPRQNDKGHTEYYSKISGQVIFSDGLLD
                     VEPLFVVEKNVDMTVGNIDFVGNVLVKGNVNDNFTVKAGGDIEVRGTVSAAHLIAGRD
                     IYVKGGVIARSAGGLRAGNNVVVRFAENASIIANQTITIDRAAMLSEIAAGDSIICKK
                     EKGQIIGGHTIARNLIEVKELG"
     assembly_gap    1234..1333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1334..1848
                     /locus_tag="PLD82_01105"
     CDS             <1334..1848
                     /locus_tag="PLD82_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG64018.1"
                     /translation="IIGGHTIARNLIEVKELGSSSGTKTEVTIGMDFFQMEQLNSLTE
                     ELQKFTRALEKIDGVLEKMEKARQEDGTLPEELVTSHTDLMQKKLLVQRKLHDIRKLK
                     VSVLMDVADNRGGELIVLGTLYTDVKINLGKTSLETHEQRDRQHAHADPQTGKLIIEP
                     WVPPHDTSKR"
     gene            complement(1859..2542)
                     /locus_tag="PLD82_01110"
     CDS             complement(1859..2542)
                     /locus_tag="PLD82_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG64019.1"
                     /translation="MFERLADRFVLGRRMERFKEVLEQRTLWIVPVLENIRKAHNASA
                     VVRTCDALGVAAVHFVEYENSFALNPKISKGAHDWVEQRAWNSMEDCLAWFRSQGYLI
                     AGTTLHESAIAPEALPLDRPVALILGNERFGLSGKAVAACDCLVSLPMYGFVESLNVS
                     VAAALVIDGHLKRVKAQGDGRWRLPLRERKRILRRWIFFCTRIGGLVQKMRLKKRIEA
                     GIAAGDDED"
     gene            2694..4316
                     /gene="aepX"
                     /locus_tag="PLD82_01115"
     CDS             2694..4316
                     /gene="aepX"
                     /locus_tag="PLD82_01115"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOG64020.1"
                     /translation="MKKTTQLKKLLMDKQVSVMMEAHNGLTAKLVEEAGFSSIWGSGL
                     SISAALGVRDNNEASWTQVLEVLEFMSDATNIPILLDGDTGYGNFNNLQRLVQKLEQR
                     NIAGVCIEDKLFPKTNSFIGGEQQPLAPVEEFAMKIRAGKDIQTDPDFVIVARTEAFI
                     AGWGLEEALRRANAYADAGADAVLIHSKAKGPEEIFAFLEHWKRETPVIVVPTMYPGV
                     TLQELQERGVSLAIWANQTCRTVVTAVQQTLKTLRESGTLLSLEDKIVPVKELFRLQG
                     MAELKEKEKLYLPATGETTSVVILGATQGKGFGKMTENRPKTMIPIGGKPILAKLVAS
                     FNDCGLKQIAAVRGYKKESVNLPNIKYFDNDTYESTEEASSLYAAKSFINGNLVFSFG
                     DILFEKFVLLNLLDSQEDFTIVVDSSWSKHPEKHSRTTDLVIADREHTREYREDNIYV
                     RSIGNEIDKAGAHGEWIGLAMFSARGAGILVETLDELAAAGRLETMPMVGVFAELMKK
                     GHRIKVEYVHGHWLDIDNLDDISDAYNFEERH"
     gene            complement(4784..5668)
                     /locus_tag="PLD82_01120"
     CDS             complement(4784..5668)
                     /locus_tag="PLD82_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOG64021.1"
                     /translation="MATYIKPWADIFIRYLFGRQANEKLLLSFTNAVLEDSGIPTIVA
                     LEIKNPFNLAEELLTKESILDIKATDENGTIYDIEVQTGGDPFYRNRSLYYWAKNYGG
                     QLDRGDEYAELKPVICINILNFVLIKELPAMHTCFMLLEKDNPDYVLTRHCMLHFLEL
                     PKMNKKKNFSNNLERWLTFLKSEGQEEETMKVIINDDAIFAKAHEEYKTFTQDDRMRE
                     LYEAREKALKDRNTLLRIAEERGIEKGIEQGIEKGIEKGIRENARRMLEDGVPMNTIA
                     KYTGLSIPEIEALRNAAD"
     gene            complement(5881..6264)
                     /locus_tag="PLD82_01125"
     CDS             complement(5881..6264)
                     /locus_tag="PLD82_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64022.1"
                     /translation="MFSDIHTFLSIGILPGMVEDRKFGKWLIGGLEGPKFSNLRKKCP
                     GLQSGSFLHSFWRRPTVPAVFEIPVFPISLRILPWRVFEESPPTSNARPCGAWWAVSF
                     VETRLIASMDTPTKYLAGDDIRLMP"
     gene            6265..6729
                     /locus_tag="PLD82_01130"
     CDS             6265..6729
                     /locus_tag="PLD82_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64023.1"
                     /translation="MRLLLPLALSGGIALMAFQFFFGKLGVVESWEKQRTIAALQQDI
                     AAIKTASAERQTQISLLSSDKGTIRGLALLYGMTGGEGIPARPLPQTTKLKRENAREG
                     QMEQPFLLRHPILIIIPGVILALLTGFTLTRRLRKQDADPQRTRYIKPSWTA"
     gene            complement(6730..7323)
                     /locus_tag="PLD82_01135"
     CDS             complement(6730..7323)
                     /locus_tag="PLD82_01135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG64024.1"
                     /translation="MRKLSMQEDGLDACIRAAGDVLVAGGLVVGPTDTLYGVFAAWDD
                     PQAVERLYTLKGRDQEKRLLALVADRRMAESVSGIPLPAGVLHYWPGPLTVILPVNAH
                     PLGWETQAIRFPDDPFSRGLAHVLGKPVYAPSANPQGMAPALDVEQAQEMFEDAVELY
                     IDGGTSGSDAPSTIVSLVGSPTLVRQGILEVMLEDFV"
     gene            complement(7333..7623)
                     /locus_tag="PLD82_01140"
     CDS             complement(7333..7623)
                     /locus_tag="PLD82_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64025.1"
                     /translation="MKMIMVVYNHSLDEELMDVLKECGVEFHTHFARATGVGGQGPHM
                     GDNIWPSLNNVLYVMVQTETLAKKVLAGIKELKSHFKTEGIQAWAWKIDSEV"
     gene            complement(7620..>10204)
                     /locus_tag="PLD82_01145"
     CDS             complement(7620..>10204)
                     /locus_tag="PLD82_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG64026.1"
                     /translation="NVLRAENINASGGTIKSGYSQFTIRTEGEFKNVDDVRNIIVTYQ
                     RGIPVYLRYVADVNWGISEEVSTSRFNNKPGLRLVVYKQSGANTVQAVDSLRAKLERI
                     RAELPAGVQLLEAFSSADFTRNSIGNTASSAILGGILAVIIVLIFLRNIRASMIIGLA
                     IPTSIIATFIGMYFFKVNLNMISLGGLTLGIGMLVDNAIIVLENTFNYMSRGAKPAEA
                     ARLGAQEIAGAIFASTLTTVCVFLPIVFTDGMAKEIFKDMALTVSFSLMASIFVAITL
                     VPMLSAKYLRFHDEKELKKLEPRLYKIVSFGERILEYLESRYKEGIRWALSNRKKLLI
                     AVVGIFLFTMMVVMRGVDSEFFPESDQASIKVTVTMPVGTRLETTNEAVEQMIGLLKK
                     AVPEKYIRNVFFLSGSPGGFSAIFNASGSHVATMQAKLVPKNERNQTTTEYIKTLKDL
                     VQKAPAPLGVAAVNISGRIGPGFGGGAPIDILVRGYDLKKGAEISKQLKTIMEKVPGL
                     YNIDVSRKEGAPEMSIRVNRVKAASLGINMATIATTIQQSVLGRTATYLREGGREYDV
                     LVRLDEKDRKSIEDIENIQVLSGYTRRPVRIGNIAQVVRDVGPINIERDDQERVIHVT
                     CSSFRGLQQSVDDIQAEVKKQIVLPAGFTLEYKGNFKDMQDTFGDLILAMIVAIALVY
                     AVMASIFESYIDPFIIFITIPLSLIGVIWMLFLTGTSLNVTSMIGILVLAGVVVNNGI
                     VLVDYINILRARGVKLEEAIMEGGRRRLRPILMTTLTTVIALVPGAIGMGEGDEANIP
                     LARTVIGGLSASTFLSLFVVPVIYLSFDHISEKRRKKRQGKTPLIRRIGHKLVSIIFR
                     EKKR"
BASE COUNT         2395 a         2913 c         2697 g         2099 t
ORIGIN      
        1 aattccgcgg aacgatcgac atgacggatg tcgttgccat cggacgggct gtcaagggaa
       61 tgccgcttct gcagctcaaa ccggccatct cgggaagaaa cggcatgacc gtcagagggg
      121 aagaaatcaa ggccgccgat gtcaagagtc tccagatcga gacaaggcgc aacaccaaac
      181 gcgccggcga cctgatcgtc cccgaactcg acggtatcgt ctacctctac gattccgcga
      241 tcctcgtcaa ggcctatgcc gatgccgaag tccagatcga gctcgacgac gaaaagatga
      301 atgcctttgt caacctgatc ccgtcggtcg gcgatgggct caagctcgac atgggtatcg
      361 cccgcgaggc actgacgaag gccggcatca ccaatggtgt gaacgagcgc atgctcgagg
      421 atgccatcac caagtgcgag gccgacaaaa tgccctttcc cgcctccccg tcgccagggg
      481 aaccaagccg gtcaacggct cggacggcaa aatcatgttc aagatccgca tgcagggctc
      541 caacgaaccg gtcgagaaag atggcggccg tgtcgattac cgcgaaaaag attcgatcat
      601 caacatcgac gagaataccc tgattgccgt ccagttcccg gcccagaaaa agatttccga
      661 cggaatgacg gttacaggcg aaaccatccc gggcacggac gggaacccgg tcgatatcaa
      721 gcttggcaag aatatcgacc ccagacagaa cgataagggc cataccgagt actactcaaa
      781 aatcagcgga caggtcatct tctccgacgg actcctggat gtcgaaccgc tctttgttgt
      841 cgaaaaaaac gtcgacatga cggtgggaaa catcgacttt gtgggaaacg tcctcgtcaa
      901 aggcaatgtc aacgacaatt tcaccgtcaa ggccggtggc gatatcgagg tccggggcac
      961 ggtcagcgcg gcccacctca tcgcgggccg ggacatctat gtcaaagggg gtgtcatcgc
     1021 ccgctcagcc ggtggactca gggccggaaa caatgtggtc gtccgtttcg ccgagaacgc
     1081 aagcatcatc gccaaccaga cgatcacgat cgacagggca gccatgctca gcgagattgc
     1141 cgcaggcgac tcgatcatct gtaaaaagga aaaagggcag atcatcggcg gacataccat
     1201 cgcccgcaac ctgatcgagg tcaaggaact cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnagatcat cggcggacat accatcgccc gcaacctgat cgaggtcaag
     1381 gaactcggat cctcctcggg aacaaagacg gaagtcacga tcgggatgga cttcttccag
     1441 atggagcaac tcaacagcct gacggaggaa ttgcagaaat tcacccgggc tctggagaag
     1501 atcgacggtg tcctcgaaaa aatggaaaaa gcccggcagg aagacgggac tctccccgaa
     1561 gagctggtca cgagccacac cgatttgatg caaaaaaaac tcctcgtcca gcgcaagctc
     1621 catgacatcc gcaagctaaa agtcagcgtc ctgatggatg tggctgacaa ccgcggagga
     1681 gaactgattg tcctggggac actctacacc gatgtcaaga tcaacctcgg gaagacttcg
     1741 cttgaaacgc acgaacagcg cgaccggcaa catgcccatg ccgacccgca gactggcaag
     1801 ctgatcatcg aaccctgggt gccgccacac gacacttcaa aaagataggc agggatcatc
     1861 aatcctcgtc atcaccggcg gcgataccgg cctcgatccg ttttttcagg cgcatcttct
     1921 ggacgagtcc accgatccgt gtacaaaaaa agatccagcg ccgcaggatc cgcttgcgct
     1981 cccggagagg gagccgccag cgcccgtcac cttgggcctt gacacgcttc aagtgcccgt
     2041 cgatcaccag ggcggcggcc acggagacat tcagactctc gacaaatccg tacatcggaa
     2101 gactgacaag acagtcacag gccgcaaccg cctttccgga caggccaaac cgctcgttcc
     2161 ccagaatcag ggccaccggt ctgtcgaggg gcagtgcctc gggagcgatc gcgctttcat
     2221 gaagagttgt acccgcaatg agatagccct gggacctgaa ccaggccagg cagtcctcca
     2281 tggagttcca ggcccgctgt tcgacccagt catgggcccc tttggagatc ttgggattga
     2341 gggcaaaact gttttcgtac tcgacaaagt ggacggccgc gacaccgagt gcgtcgcagg
     2401 tccggacaac ggcactggcg ttatgggcct tgcggatatt ctccaaaaca ggaacaatcc
     2461 agagggtccg ctgttcgaga acctctttga atcgctccat gcgtcgcccg aggacaaagc
     2521 ggtcggcaag acgttcaaac atggtcacag gcaaactccg ggcgggaatg gtatcccgaa
     2581 aaggtagtca cccgttccat tcgtaagtag tgcagaacac gggttttttt actattattc
     2641 tcatggcctg tcagcgattc tggcggcccc aaaccgtatt gacggagttc cccatgaaaa
     2701 aaacaaccca gttgaagaag ctgctgatgg acaagcaggt cagcgtcatg atggaggccc
     2761 acaatggcct gactgcaaaa ctcgtcgagg aagccggctt ctcctcgatc tggggcagcg
     2821 gtctctccat ttcggccgcc ctcggtgtcc gcgacaacaa cgaggcatcc tggacacagg
     2881 ttctcgaggt cctcgagttc atgtccgacg ccaccaatat ccccatcctc ctggacggcg
     2941 ataccggcta cggcaatttc aacaacctcc agcgcctggt tcaaaagctc gagcagcgca
     3001 acattgccgg tgtctgcatc gaggacaagc tcttccccaa gaccaactcc tttatcggtg
     3061 gtgagcagca gcctttggcg cccgtcgaag aatttgccat gaagatccgg gccggaaaag
     3121 acatccagac cgatcccgac ttcgtgatcg tggcccggac cgaagccttc atcgccggct
     3181 ggggactgga agaggccctg cgccgcgcca acgcctacgc cgatgccgga gcggacgccg
     3241 tcctgatcca ctccaaggcc aagggtcccg aagagatctt cgccttcctc gaacactgga
     3301 agcgcgagac cccggtgatt gtcgttccca cgatgtatcc cggcgtcaca ctccaggaac
     3361 tccaggaacg cggtgtctcg ctcgccatct gggccaacca gacctgccgg accgtggtga
     3421 ccgctgttca gcaaaccctc aagaccctgc gggaaagcgg gaccctgctc tcgcttgaag
     3481 acaagatcgt gccggtcaag gagcttttca gactccaagg gatggccgag ctcaaggaaa
     3541 aagaaaagct ctacctcccg gctaccggcg aaacaaccag tgtcgtgatc ctcggcgcaa
     3601 cacaaggcaa gggctttggc aagatgaccg aaaaccggcc caagaccatg atcccgatcg
     3661 gcggcaaacc gatcctcgcc aagctggtcg cctctttcaa cgactgcggg ctcaagcaga
     3721 tcgccgccgt acgtgggtac aaaaaggaat ccgtcaacct tcccaacatc aagtacttcg
     3781 acaacgatac ctacgagagc accgaagagg cctcatccct ctacgccgcc aagagcttta
     3841 tcaacggtaa cctcgtcttc tcctttggcg acatcctctt cgaaaaattc gttctcctca
     3901 acctcctcga cagccaggaa gatttcacga tcgtggtcga ttcaagctgg tccaagcacc
     3961 ccgaaaagca cagccgtaca acagatctgg tcatcgcgga tcgcgagcac acccgcgaat
     4021 accgcgagga caatatctac gtccgctcga tcggaaacga gatcgacaag gccggcgcac
     4081 atggcgaatg gatcggactg gccatgttct cggcccgtgg agccgggata ctcgtcgaaa
     4141 cgctggatga actggccgca gccggtcggc tcgagacgat gcccatggtc ggggtctttg
     4201 ccgagctgat gaaaaagggc caccggatca aggtcgagta tgtccacggc cactggctgg
     4261 atattgataa tctcgacgat atctcggacg cctacaactt cgaggaacgg cactgatccc
     4321 ccgatcatcc gccattcgtc ccgcaaaccg cgcgtttttt gcgcggtttt tcttttttct
     4381 gaacatcctc aggcccgcga cgggcggcct tgtctcggtt gggtatgcag gcgatacgcg
     4441 ggaacagctt tgtgctttgt ccacacgcct gcgccggaag tgcgcatgcc aatatcgcac
     4501 ctcggagacg cgaccaatcg cgtctctaca aatacaacgc catccaaaga tgtacacgcc
     4561 tcctcccccg cagggggagg ctgggagggg gaaacgcctg cgtttctcca ccggatttcc
     4621 ttggcatatc acggtttttt cataatgccc gatgattctc gtaaggacaa tattcaatcc
     4681 gggacacttt ttttctcaaa tttgagaaaa tccccttttt ctacacctac tatgtaatcc
     4741 gggatttttt ccaatttgag aattacgcca aattttttat cgttcagtcc gctgcattcc
     4801 gtaatgcttc gatttccggg atggagagtc cagtgtattt ggcaatggtg ttcatgggta
     4861 cgccatcttc aagcatgcgc cgtgcattct ccctgatgcc tttttcgata cctttttcga
     4921 tgccctgttc gatgcctttt tctatgcccc gttcttcggc gattctgagg agggtgttgc
     4981 ggtctttgag ggctttttcg cgggcttcat agagctcgcg catgcggtca tcctgggtga
     5041 atgtcttgta ttcttcgtgg gccttggcaa aaatggcatc gtcgttgatg atgactttca
     5101 tggtttcctc ctcttggcct tcgctcttca aaaacgtcaa ccaacgctcg agattattgg
     5161 aaaagttttt cttcttgttc attttgggca gttcgaggaa atgcagcatg cagtggcggg
     5221 tcaggacata gtcaggattg tccttttcaa gcagcatgaa gcaggtgtgc atcgccggca
     5281 gttctttgat gaggacaaag ttgaggatat tgatgcagat cacaggcttg agttcggcat
     5341 actcatctcc tctgtccagt tgtccgccgt agttttttgc ccagtaatac agcgagcggt
     5401 ttcggtagaa cggatctccg ccggtttgaa cctcgatatc gtagatggtg ccgttttcat
     5461 ctgtggcttt gatgtccaag atggattctt tggtcaagag ctcttcagcc agattgaagg
     5521 gattcttgat ctcaagggcc acgatggtcg gaatgccgga gtcctcaagg acggcattgg
     5581 taaaagacaa gaggagcttc tcgttggctt gtctgccaaa aaggtaccgt atgaaaatat
     5641 ccgcccatgg tttgatgtac gttgccataa caaaagtata cttcaaaagt gcaaatgcag
     5701 caatgccggg gtgtacggcg tggctgggtg tccctactca atttcgcgaa accatgccaa
     5761 tccgggatta cacagcagca ccccgttact gcaaaaatac tccctgaaaa acacgggaca
     5821 ccgggagggg aaaacccatg cttcgcgtcc ccgaaattcc atgcacaaat aacggttttg
     5881 ttatggcatc agacgaatat cgtcccctgc caggtacttc gtgggagtat ccatagacgc
     5941 gattaatcgt gtctcaacaa acgaaaccgc ccaccacgct ccgcaggggc gcgcgttgct
     6001 ggtcggaggc gattcctcaa aaacacgcca agggaggatc cgcagactga tcggaaagac
     6061 cgggatttca aatacagccg ggacagtcgg cctgcgccaa aaactgtgta aaaatgatcc
     6121 gctttgtaaa ccgggacact tttttctcaa atttgagaat ttcggccctt cgagacctcc
     6181 aataagccat ttcccgaatt ttcggtcctc aaccatgccc ggcagaatgc cgatactcaa
     6241 aaaggtatgg atatcagaaa acatgtgcgg ctcctcctcc cgttggccct ctccggggga
     6301 atagccctga tggccttcca gtttttcttc ggaaaactgg gagtggttga gtcttgggaa
     6361 aaacaacgca ccatcgccgc actgcagcag gatatcgctg ccatcaagac ggcatccgca
     6421 gaacgccaga cccagatcag cctgctctcg tcggacaagg gaacgatccg tggtctggcc
     6481 cttctctacg ggatgaccgg cggggagggg ataccagcgc gaccactccc gcagacgaca
     6541 aaactgaagc gggagaacgc gcgagagggg caaatggaac agcccttttt attgcggcac
     6601 ccgatcttga tcatcatccc gggagtgatt ctggcactcc tgaccggctt cacgctgacc
     6661 cgtcgcctgc gcaaacagga tgcggatcca cagcgaaccc gctacatcaa accttcctgg
     6721 actgcctgat cagacaaaat cctcaagcat gacttcaagg atcccctgac ggaccagggt
     6781 tggagaaccg accagcgaaa cgattgttga aggggcatcg gaaccggatg taccgccatc
     6841 gatatagagc tccaccgcat cctcgaacat ttcttgggcc tgttccacat caagagcggg
     6901 cgccatcccc tgcggattgg cactgggcgc atagaccggt tttcccaaaa catgggccaa
     6961 tccccgggaa aaaggatcgt cgggaaaccg gatggcctgt gtctcccagc caaggggatg
     7021 cgcattcacg ggaaggatga cagtcagcgg acccggccaa tagtgcagaa caccggccgg
     7081 aaggggaatc cctgaaacgg attcggccat cctccggtcc gcgaccaggg ccaggaggcg
     7141 tttctcctga tcccgcccct tgagtgtata gaggcgctca acagcctgcg ggtcatccca
     7201 cgcggcaaag acaccataga gggtatccgt cggtccgacc accagaccac cggcgaccaa
     7261 aacgtcaccg gcggcccgga tacaggcgtc gagtccatct tcctgcatcg aaagtttccg
     7321 catgctgttt cctcaaacct cagagtcgat cttccaggcc caggcctgga tgccctcagt
     7381 cttgaaatga ctcttgagct ccttgatccc ggccaggact tttttggcca gcgtctcggt
     7441 ctgcaccatg acatagagga cgttgttgag cgacggccag atgttgtccc ccatgtgcgg
     7501 gccctgcccg ccaacgcccg tggcccgggc aaagtgggta tggaattcga ccccgcactc
     7561 cttgaggaca tccatcaatt cctcgtccag cgagtggttg tagaccacca tgatcatctt
     7621 catcgtttct tctcccggaa tatgatggat acaagcttgt gcccgatgcg ccggatcaag
     7681 ggggttttcc cttggcgctt tttccggcgc ttttcggaga tgtggtcaaa gctcagataa
     7741 atgacgggaa ccacaaagag tgagaggaag gtcgaggccg agagaccgcc gatgacggtc
     7801 cgtgcaagcg ggatattggc ttcgtccccc tcacccatcc cgatggcacc aggcacaaga
     7861 gcgatcaccg tcgtgagtgt cgtcatcaaa atcgggcgga ggcggcgacg gcccccttcc
     7921 atgatggctt cttcgagctt gacaccacgg gctcggagaa tattgatata atccactaac
     7981 acgataccat tatttaccac cacaccagcc aacacaagaa taccgatcat cgaagtgacg
     8041 ttgagtgagg ttccggtcaa aaagagcatc cagatcacgc ctatcagcga aaggggaatc
     8101 gtgatgaaga tgatgaacgg atcgatgtag gattcgaaaa tcgatgccat cacggcatag
     8161 accagggcaa tcgcgacgat catggccagg atgagatcac cgaaggtgtc ctgcatgtcc
     8221 ttgaaattcc ccttgtattc gagggtaaat ccggccggca ggacaatctg ctttttgacc
     8281 tcggcctgga tgtcatccac ggactgctgc agccccctga aggatgaaca agtgacatgg
     8341 atcacgcgct cctggtcgtc acgctcgata ttgatcggac cgacatcgcg aacgacctgg
     8401 gcgatattcc cgatccggac cggtcttcgg gtgtaccccg agagaacctg gatgttttcg
     8461 atatcctcga tgctcttgcg gtccttctcg tcaaggcgga caagcacatc atattcgcga
     8521 ccgccttccc tgaggtaggt tgctgtcctc cccagaacac tctgctggat ggtcgtggca
     8581 atcgtcgcca tattgatccc gaggctggcc gccttgaccc ggttgacccg gatcgacatc
     8641 tcgggagccc cctccttgcg actgacatcg atgttgtaca ggccgggaac cttttccatg
     8701 atcgtcttga gctgtttgga gatctcggcg ccctttttga ggtcataccc acggacaaga
     8761 atgtctatcg gagccccgcc accaaacccg gggccgatcc gtccggagat attgaccgct
     8821 gcgacaccca gtggagccgg agccttttgt acgagatcct tgagcgtctt gatgtattcc
     8881 gttgtcgtct gattacgttc gtttttgggg acaagcttgg cttgcatggt tgcgacatgc
     8941 gacccggaag cattgaagat ggccgaaaaa ccgcccgggc tcccgctgag aaaaaacaca
     9001 ttccgtatgt acttttcggg aacggccttt ttgaggagtc cgatcatctg ctcgaccgct
     9061 tcattggtcg tctcgagcct ggtcccgacc ggcatggtca cggtcacctt gatggaggcc
     9121 tggtccgact cggggaagaa ctccgaatcc acaccacgca tgacgaccat catggtaaag
     9181 aggaagatac cgacgactgc aatgagaagt tttttccggt tggagagagc ccagcggatc
     9241 ccctccttgt atctcgattc gagatactca agaatccgtt cgccaaagct gacgatttta
     9301 tagagccgtg gttcaagctt tttcaattct ttttcgtcat ggaagcgcaa gtatttggct
     9361 gaaagcatcg gaaccagcgt gatcgccaca aaaatggacg ccatcaacga gaaggaaacc
     9421 gtcagcgcca tgtccttgaa gatctctttg gccatcccgt ccgtaaacac gatgggcaga
     9481 aacacgcaga ccgttgtcag ggtcgaagca aaaatggccc ccgcaatttc ctgggcaccg
     9541 agacgggccg cctccgccgg cttggcacca cgactcatgt aattgaaggt attttccagt
     9601 acgataatgg cgttgtccac caacattccg ataccgagtg tcagacctcc cagtgaaatc
     9661 atgttgaggt tgaccttgaa aaaatacatc ccgataaagg tcgcgataat cgatgtcggt
     9721 atcgccagtc cgatgatcat ggatgccctg atgttgcgca gaaagatcag cacgatgatc
     9781 acagcaagga taccacccaa aatcgccgaa ctggccgtgt tcccgatcga gtttctagtg
     9841 aagtcggccg aactgaaggc ttcgagcaat tgcaccccgg ccggaagctc ggcccggatc
     9901 cgttcgagct tggcccgaag actgtccaca gcctggaccg tattggcacc ggactgcttg
     9961 tagacaacga gtcgcagtcc gggtttgttg ttgaacctgg atgtcgagac ctcctcggag
    10021 attccccagt tgacatcggc aacatagcgc agataaacag gaatccctct ctggtatgtg
    10081 actatgatgt tccggacatc gtcgacgttc ttgaactcac cctcggttct gatggtgaac
    10141 tggctatagc cgctcttgat ggtcccgccc gaagcattga tgttctcggc ccggagaaca
    10201 ttga
//
LOCUS       DAOSHE010000019        10046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5708_length_10046_cov_4.144630, whole genome shotgun sequence.
ACCESSION   DAOSHE010000019 DAOSHE010000000
VERSION     DAOSHE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_10046_cov_4.144630"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLD82_01150"
     CDS             <1..705
                     /locus_tag="PLD82_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG64027.1"
                     /translation="PPFVIDTNEYLIVKGDDAILTDPGGLEIFPVVFTAVSQEFNPEQ
                     INKIFSSHQDPDIISSLGLWLEVNPDIRCHLSWLWRTFVPHFGGTRDTFVSIPDEGVR
                     IPLGDIELEAIPAHYLHSCGNFNLWDPKAKILFSGDIGAALLPPGKIHDLFVSDFDAH
                     LPYITVFHQRWMASNQAKNDWCRRVRELKPEMICPQHGSIYKGEMVTRFIDWFEALEV
                     GVLGVTPPALDLSGRG"
     gene            complement(712..1179)
                     /locus_tag="PLD82_01155"
     CDS             complement(712..1179)
                     /locus_tag="PLD82_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64028.1"
                     /translation="MSYAQFFDITFSKPVRSVALFGMSLFLMILLSCSRKSEHVVTGR
                     GLDPQAHAPREVTDSFAAGEPFSLVLAPVRFEDSYLDLIIWTWGTNPSPKRTRTITLK
                     GLDTREEYLQISNAFSIPFPGRYRISFEQLGKTLGWADVTITPTGIAPGPEKR"
     gene            complement(1257..1339)
                     /locus_tag="PLD82_01160"
     tRNA            complement(1257..1339)
                     /locus_tag="PLD82_01160"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Tyr,seq:gta)
     gene            complement(1353..1425)
                     /locus_tag="PLD82_01165"
     tRNA            complement(1353..1425)
                     /locus_tag="PLD82_01165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1390..1392),aa:Thr,seq:tgt)
     gene            complement(1475..1744)
                     /locus_tag="PLD82_01170"
     CDS             complement(1475..1744)
                     /locus_tag="PLD82_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64029.1"
                     /translation="MLDTEILQDALKRGAISERKLKTLSILKTCDNWKKVTPVGLIDH
                     RTVLTAYQGMLVELDKRAYFVRRETLAALKNEIKWNVTRMIRIEQ"
     gene            complement(1737..3545)
                     /locus_tag="PLD82_01175"
     CDS             complement(1737..3545)
                     /locus_tag="PLD82_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64030.1"
                     /translation="MGTNGDQDRLSAYKLYSLVNISKKINSLLEINALLELIMESCKT
                     LLNAEASSLMLVNEEKQVLTFNIISGKGEELRTMEVPIGKGIAGIVAQSGEPLIINDA
                     QNDPRFFKEADKKTNMVTSSIVCVPMTTNKRVIGVLEAINALDRDGFNDDDLLLLMAL
                     AEQAAISITNRKLYDRIKARAEELSAMYEIGQLAMSHGSPERILQESLEIVSRVMGCK
                     RTSILMHDASDDTLRVKASIGFEGDVSGQIKVTPENEISYQVFHTLKFIYTENVDTDP
                     RFGRNKRFRYTTRSFVSVPMQTKERPIGVLNVTDKQDGGHFDAHDVRLLQTIANQISE
                     VHENIRLYNEERVKLKLEKELEVTRRLQEAIFPKSFPSIPGVTMFACNKPASEVGGDF
                     YDFFHSTEDPGQFTAVIADVSGKSVPAALFMAISRSILRAQAVSQEKPDPAALFESAN
                     RLIIADSDSAMFVTSFMLHCDINARKAVYSNAGHNDPILLRRGRDHCIQLHTRGKPLA
                     VMPDSVYQNSVLDIEPGDIIVMYTDGIVEANNMLEQEFGMDRFEEEILACRHLPADAM
                     ARHILQRVNAFAAETPQFDDMTLFIFKFELEPNLHA"
     gene            3544..5019
                     /locus_tag="PLD82_01180"
     CDS             3544..5019
                     /locus_tag="PLD82_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HOG64031.1"
                     /translation="MPAALPESARKGLNASASRIYITAMTRIRIIASIIIGKMLILLA
                     RLLRRGGSSLPGRVALRIAPGLLCTLAARIEGGVVAVTATNGKTTTTRMLAGLVRHAG
                     RPVVTNASGANMAGGIVSAMIEAASLRGRFPRQALGIIEVDEGSIPAVLPLLRPMLLL
                     VGNFFRDQLDRYGEVAILARRVGETIATLANPPRLVINADDPIAASLADTGSGLPVWF
                     GVDAVAETVPVSGGETADIRHCPVCHAPLEYSLRVMGHLGRYHCRGCGRQRPEPDWSA
                     GRTLALGMMQQEYCLAHRGGQSFTVRLSFPGIHAVYNSLGALAVLDQLGLLDQGAIDW
                     LAGFIPPYGRFEKIDLAGRMVFLMLVKNPAGANASLDILAQHAPGGDVLILLNDLAAD
                     GRDISWIYDAGFESIAGAGNVTTGGRRADAMALRMLHAGVPGSRIESAGGDIPRALEM
                     AMSKTDPGKSLAIIATYTAMHAIRAVLVRRAGIRDFWKDGT"
     gene            5016..5789
                     /locus_tag="PLD82_01185"
     CDS             5016..5789
                     /locus_tag="PLD82_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOG64032.1"
                     /translation="MMDCCLRVGHLYPDHMNLYGDRGNVMAFVYRAGLRGIRVSVEPL
                     QPGDRIEPGRYDFFFFGGGQDAEQERIYEDFLSVKGGQLARELDSGAACLAVCGGYQL
                     LGKSYEAFGGKIIPGLGWFDVRTEAGKTRAIGNILIESDINGNRVELAGFENHGGMTF
                     LGPGVSPLGRVLVGSGNNGQDGSEGALVQAVVGTYLHGPVLPKNPVLADWLVARSLEH
                     RYPGRVLEDVESRFEDAAFSEARRITLEGRGVQREQFGR"
     gene            complement(5796..6104)
                     /locus_tag="PLD82_01190"
     CDS             complement(5796..6104)
                     /locus_tag="PLD82_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64033.1"
                     /translation="MGVRTESAVSGEEAVRANDLKKYDIIFLDYLMPGMGGIEAATSL
                     RTRGYSGAIIIISGRTILPSLKNALEIQRADYLAKPFEISEVRELLQRISRRQQTTPE
                     "
     gene            complement(6148..7335)
                     /locus_tag="PLD82_01195"
     CDS             complement(6148..7335)
                     /locus_tag="PLD82_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64034.1"
                     /translation="MKRHILIIDDDISHASFMMMLLEDAGYLCSRIASGEKALERLQD
                     DLRPDLILLDIMLEGIDGYEVCKRIKTDTRLRAIPVIILSGRSSIDEKILGLEIGADD
                     YLVKPVKTRELLARISVLIRLRSLQEQITQVEKLAGLGQLAAGIAHEFNNLIGGMLGY
                     AQLAMLTPDNREMTLKALSVVEKSCFRAKELTENMLLFSTHSDQPEGQADVASTMEHT
                     SVLLSSQLSKNRITLTQEFTHKRTLAITANRLLQLLMSLMQNAIHAILENGGEEREIL
                     VSTRETDEVVQISIRDTGCGIPQNQRSRIFEPFFTTKGPLGGGRYGCHRNRSFRRPRN
                     RLILRRPSGTGRVRREKGKHLQADNSCLCRERKKSRSNHRPGITADCPWAHTRRTLGP
                     HRR"
     gene            complement(7397..8929)
                     /locus_tag="PLD82_01200"
     CDS             complement(7397..8929)
                     /locus_tag="PLD82_01200"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOG64035.1"
                     /translation="MTEPLDRKKQGEVRALLFSILLTSVCAITYELLIGAVSSYLTGN
                     SVVQYSLTIGIFLSAMGLGSWLSRWLTGDLVAIFADVELGLGLLGGLSLIILYSVYLL
                     GDLYMPVFILLSASMGTLIGFEIPLVTRIMRSYGPLSSSLSTVLTFDYVGGLAASLLF
                     PFILLPVFGMIRTAFVTGIFNILVVLVLLNVFRERIARPLRIGATLGGLLALCSVFFA
                     SDPLAAWYEQQLYQDTVMHSEQSQFQKIVLTGWRDDTRLWLDGSLQFSTVDEYRYHES
                     LVQPAMTALPGIERALVMGGGDGMAVREILKRPEVKEIHLVDLDPAITRLAQTHPALV
                     AANSNALADPRVRIFNKDAFKFLEETGTGLYDLIVADFPDPHNEVLSKLYSREIYRLV
                     RHRLARAGVFVTQAASPFFAREVFWMIHATLSEVFPHTVPYHENVPSFGDWGFVMASA
                     IRWQPDWTRLPARLRHITPAVFSNALGFAPDSTRPKNRIINTLNTPRIYQAYQRGWKN
                     YN"
     gene            complement(8926..9303)
                     /locus_tag="PLD82_01205"
     CDS             complement(8926..9303)
                     /locus_tag="PLD82_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOG64036.1"
                     /translation="MEKIIEGILSSAIYGILGIILMGLGFLIVRVVTPFSIKKEIEVD
                     QNTSLGLIIAAVIIGISIIIAAAIFSFENKVESNLQVSGSIERIETKSYDSAGKVIRT
                     ETKIYKAEAPAAKPAPAKDDKSK"
     gene            complement(9395..9595)
                     /locus_tag="PLD82_01210"
     CDS             complement(9395..9595)
                     /locus_tag="PLD82_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64037.1"
                     /translation="MIKTAIMLLALGGIGLYGTIAKPFIPRSGTEFIRKTPGLSQPGK
                     GSPAHRTTGYRSHYGGGPRLGK"
     gene            complement(9623..>10046)
                     /locus_tag="PLD82_01215"
     CDS             complement(9623..>10046)
                     /locus_tag="PLD82_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64038.1"
                     /translation="EEAGPIHQLRLAAPSIHNEWIESTAVLSRVVSPTSNELVYSFTA
                     GIWHASGTDSEGYWSESGGNDSEEFVLDKNGTYTIELSITSEKSNRTVADVAVMIRDG
                     ERSYSFLLVAGLLSLFLALRARSRRKTTGSIPGGLLPD"
BASE COUNT         1956 a         2706 c         3143 g         2241 t
ORIGIN      
        1 ccgccctttg tcatcgatac caatgaatat ctgatcgtca agggggatga cgccatcctg
       61 acggatccgg gcgggctgga gatctttccc gttgtcttta ccgcggtcag tcaggagttc
      121 aatcccgagc agatcaacaa gattttttcg tcccaccagg atcccgatat catctcctcc
      181 ctcggtctct ggctcgaggt caaccccgac atccgttgcc atctctcctg gctctggcgc
      241 acctttgtgc cgcattttgg cggaacccgg gatacctttg tgtccattcc tgacgagggg
      301 gtccggattc ccttgggtga tatcgaactc gaggcgattc cggcccacta tctccattcc
      361 tgcgggaatt tcaatctctg ggatcccaag gcaaagatcc tcttcagcgg ggatatcggt
      421 gcggcactcc tgccgccggg gaaaatccat gatctgtttg tttcggactt cgatgcccat
      481 ctcccgtaca tcacggtctt ccaccagcgt tggatggcat ccaatcaggc caaaaacgac
      541 tggtgccggc gtgtgcgcga actcaagcca gagatgatct gtccgcagca cggctcgatc
      601 tacaagggag agatggtcac ccgcttcatc gactggtttg aagccctcga ggtcggtgtc
      661 cttggtgtga caccgcccgc cctggatctc tcggggcggg gctgagcggg atcacctttt
      721 ctccgggccg ggggcgatcc cggtcggagt gattgtgaca tcggcccacc ccagggtttt
      781 tccgagctgc tcgaaactga tccggtaacg tccggggaag ggtattgaaa aggcattgct
      841 gatttgcaga tactcttccc gggtgtccag ccccttgagt gtgatcgtgc gggtccgctt
      901 gggactcggg tttgttcccc atgtccagat aatcagatca agataactgt cctcgaaacg
      961 gacaggcgcg aggaccagcg agaatggctc cccggccgca aagctgtccg ttacctcgcg
     1021 gggagcatgc gcctgcggat cgagaccgcg gcctgtcacg acatgctcac tcttcctgct
     1081 gcaggaaaga agaatcatga ggaagagact catgccgaag agcgcgacgg accggactgg
     1141 tttggaaaac gtgatatcaa aaaactgtgc atagctcata ttgtatggat ctcctgcggg
     1201 attggtgcag atcggactcc cataaaaaaa ccgttcagcc ggaagctgaa cggttgtgga
     1261 gagagatgga ttcgaaccat cgtaggcgca agccaacaga tttacagtct gcccccttta
     1321 gccgctcggg catctctccg ggtgcatgct tgagctggag atgggactcg aacccgcaac
     1381 ctgctgatta caaatcagct gctctaccga ttgagctact ccagcgcaac gcgataatga
     1441 tagtcaggag aaggaggaac ggcaagaaaa aatctcactg ttcgatacgg atcattctcg
     1501 tgacgttcca tttgatctcg ttcttgagtg cggccagggt ttcccggcgc acaaaatagg
     1561 cccgtttgtc cagttcgacc agcattccct ggtacgcagt caggacggtc ctgtggtcga
     1621 tcaaaccgac cggggtgact tttttccagt tgtcgcaggt tttcaggatg gagagggtct
     1681 tgagcttgcg ctcgctgatg gccccccgtt tgagggcatc ctggagaatc tctgtatcaa
     1741 gcatgcaggt tcggctccag ttcgaatttg aagataaaga gggtcatgtc gtcaaactgc
     1801 ggggtctccg cagcaaaggc gtttacacgc tggaggatgt ggcgggccat cgcatcggcg
     1861 gggagatggc gacaggcgag gatctcctcc tcgaagcggt ccatcccgaa ctcctgctcg
     1921 agcatgttgt tggcttcaac gattccgtcg gtgtacatca cgatgatgtc gcccggctcg
     1981 atatcgagga cagagttctg atagaccgaa tctggcatga cggccagcgg tttgccacgg
     2041 gtgtgcaact ggatacagtg gtcccggccg cggcgcagga ggatcggatc attgtgaccg
     2101 gcgttggaat agacggcctt gcgggcattg atgtcacaat gcaacataaa acttgttaca
     2161 aacattgcgg aatcggaatc cgcgatgatc aggcggttcg cggactcgaa gagggcggcc
     2221 gggtccggtt tttcctggct gacggcctga gccctgagga tcgagcggga gatggccatg
     2281 aacagggcgg ccggtacgga tttgcccgag acatcggcga tcacggcggt gaactgtccc
     2341 gggtcttcgg tcgaatggaa gaagtcatag aagtcgccgc cgacctcgct ggccggcttg
     2401 ttgcaggcga acatggtcac gccaggaatg gaagggaagc tcttgggaaa gatcgcctcc
     2461 tggagcctgc gggtgacttc gagttccttt tcgagcttga gcttgacccg ctcctcgttg
     2521 tagagccgga tgttttcgtg cacctcggag atctggttgg cgatggtctg gaggagacgg
     2581 acatcatggg catcaaaatg gccgccatcc tgcttgtcgg tgacattcag gaccccgatc
     2641 gggcgctcct tggtctgcat cggaaccgag acaaaggagc gggtggtgta gcggaagcgt
     2701 ttgttgcggc cgaagcgcgg atcggtatcg acattttcgg tatagatgaa cttgagcgtg
     2761 tgaaaaacct ggtaggagat ttcattttct ggggtgactt tgatctggcc cgagacatcg
     2821 ccttcaaacc cgatcgaggc cttgacccgg agggtgtcgt ccgaggcatc gtgcatcaga
     2881 atcgaggtcc gtttgcatcc catgacgcgg gatacgattt cgagcgattc ctggaggatg
     2941 cgctcggggc ttccgtggct catcgcaagc tggccgattt cgtacatggc cgagagttct
     3001 tcggctcggg ccttgatccg gtcatagagt tttctgttgg tgatggagat ggcggcctgt
     3061 tcggccagtg ccatcaggag caaaagatcg tcatcattga aaccgtccct gtcgagggca
     3121 ttgatcgcct cgaggactcc gatcacgcgt ttgttggtgg tcatcgggac acagacgatg
     3181 ctgcttgtga ccatattcgt ttttttgtcg gcctctttga aaaaacgggg atcgttttgg
     3241 gcatcgttga tgatgagagg ttccccggac tgggcaacga tcccggcgat tcccttgccg
     3301 atcgggactt ccatcgtccg cagctcctcg cccttcccgg agatgatgtt gaaggtgagg
     3361 acctgctttt cctcgttgac gagcatcaag gagctggcct cggcgttgag cagggtcttg
     3421 cagctttcca tgatcaattc aaggagggca ttgatctcca gcagggagtt gatcttcttg
     3481 ctgatgttga caagcgagta cagcttgtat gcggataatc tgtcctggtc gccgttggtt
     3541 cccatgcctg ccgccttgcc cgagtctgcc cgaaagggct tgaatgcatc cgcatcccga
     3601 atctatatta ctgccatgac aaggatacgg atcattgcat ccataatcat cggaaaaatg
     3661 ctcatccttc ttgcgcggct tctccgccgc gggggatcga gtctccccgg gcgggtagcc
     3721 ctcaggattg ctccggggct cctctgcacg cttgcagcgc gcattgaggg cggggtcgtt
     3781 gccgtgacgg caaccaacgg aaaaacaacg accacacgga tgctggccgg actggtccgg
     3841 catgccgggc gacccgtcgt gaccaatgcg agtggcgcca atatggccgg cggaattgtc
     3901 tcggcaatga tcgaggcagc ctcgctccgg ggccggtttc cccggcaggc cctggggatt
     3961 atcgaggttg atgagggttc gattccggcg gtccttccac tcctccggcc gatgttgctt
     4021 cttgtcggaa attttttccg ggaccagctt gaccgctacg gagaggtcgc gattctggcc
     4081 cggagggtcg gggagacgat tgccaccttg gccaatccgc cccggctcgt gatcaatgcg
     4141 gatgatccga tcgccgcttc cctggccgat actggctccg ggctgcccgt ctggttcggg
     4201 gtggatgccg ttgccgaaac tgtccctgtc agtggcggag agaccgcaga cattcggcat
     4261 tgtcctgtct gtcacgcacc cctcgagtac agtctccggg tgatgggaca cctcggccgg
     4321 tatcattgcc gggggtgcgg gcggcaacgt ccggagccgg attggtccgc gggtcgaacg
     4381 cttgccctgg ggatgatgca gcaggagtat tgtctggcac accgtggcgg ccagtcattc
     4441 acggtcagac tctccttccc cgggatccat gccgtctaca attcccttgg cgcgttggcg
     4501 gttctggatc agctcggttt gctcgatcag ggcgcgattg actggttggc cggtttcatc
     4561 ccgccctatg gccggtttga aaaaatcgac ctcgcgggac ggatggtatt cctcatgctg
     4621 gtcaaaaacc cggcgggagc caatgcatct ctcgatattc tggcgcaaca tgctcccggg
     4681 ggagatgtcc tcatcctgtt gaatgatctg gcggctgatg gacgggatat ttcctggatc
     4741 tacgatgccg ggttcgaatc gatcgccggg gcaggcaatg tcacaaccgg tggacgcagg
     4801 gccgatgcca tggccttgcg catgctccat gccggggttc ccggttcgag aatcgagtcg
     4861 gcggggggag atatccctcg cgcgcttgaa atggcgatga gcaagacaga ccccggcaag
     4921 agcctggcca ttatcgcgac ctacacggcc atgcatgcga tccgtgcggt tcttgttcgc
     4981 agggcaggga tcagggattt ttggaaggac ggtacatgat ggattgctgt ctgcgggtcg
     5041 ggcacctcta tcccgaccac atgaatctct acggtgatcg cgggaacgtg atggcctttg
     5101 tgtaccgggc cggattgcgg ggcatccgtg tcagtgtcga gccccttcag cccggagaca
     5161 ggatcgagcc cggccggtat gacttttttt tctttggtgg cggacaggat gccgagcagg
     5221 agcggatcta cgaggatttt ctctcggtca agggcgggca actcgcgcgg gaactggatt
     5281 ccggtgcggc ctgcctcgcg gtctgcgggg ggtaccaatt gctcgggaag tcgtatgagg
     5341 cgtttggcgg gaagatcatc cccggtctgg ggtggtttga tgtccggacc gaagccggca
     5401 agacacgtgc catcggaaat atcctgatcg agtccgacat caacgggaat cgggtcgagc
     5461 tggccggttt tgaaaaccat ggcggaatga cgtttttggg gcccggcgtt tccccgcttg
     5521 gccgggtgct tgtcggctcc gggaacaatg gacaggacgg gagcgagggg gccctggtgc
     5581 aggctgtcgt gggaacctat ctccatggac cggtccttcc caaaaatccc gtccttgcgg
     5641 actggctggt ggcgaggagc ctggaacaca ggtatccggg tcgtgttctg gaagacgtcg
     5701 aaagccgttt cgaggatgcc gccttcagtg aagcccggcg gatcacgctc gaagggcggg
     5761 gtgtgcagag ggaacagttc ggccgttagc gcctgtcatt ccggcgttgt ctgctggcgc
     5821 cggctgatcc gctgtaaaag ctcccggact tcggagattt cgaagggttt ggcgaggtag
     5881 tcggcccgct ggatttccag ggcatttttc agactgggga ggatggtccg gccgctgatg
     5941 atgatgatcg cccccgagta tccccgggtc cggaggctgg tagccgcttc aatcccgccc
     6001 attccgggca taagataatc caaaaaaatg atatcgtatt ttttgaggtc gttggcccgg
     6061 acggcttctt ccccgctgac ggcactctcg gtccgtacac ccaattcggt caggatgtct
     6121 tcgagaagat tccggtagat ctgctcgtca tcggcgatga ggaccgagag tcctgcgagt
     6181 gtgagcccag gggcagtcag cggtgattcc cggccggtga ttgcttcggg attttttccg
     6241 ttcccgacaa agacaggaat tgtcagcctg aaggtgcttc cctttttctc ttcggactcg
     6301 accagttcca gaaggccgcc gtaggatgag gcgattccgt gggcgacgga aagaccgatt
     6361 ccggtggcac ccatatcgcc cccccccgag cgggcccttt gtcgtgaaaa agggctcaaa
     6421 gatccttgag cgttgattct gggggatgcc gcacccggtg tcccggatgg agatctggac
     6481 cacttcgtcg gtctcccggg tcgacaccag gatctcgcgc tcttcaccac cgttttcgag
     6541 aatggcatgg atggcgtttt gcatcaggct catcaaaagt tgcaggagcc ggttggctgt
     6601 gatggcaagc gtgcgtttgt gggtgaattc ctgcgtcagt gtgatccggt ttttggagag
     6661 ctggctggag aggaggaccg atgtgtgttc catggtactc gccacatccg cctgtccctc
     6721 cggttggtcg ctgtgggtgg agaagagaag catgttttcc gtcagttcct tggcgcggaa
     6781 acagcttttc tcgacaacgg agagggcctt gagggtcatt tcccggttgt ccggggtgag
     6841 catggcaagc tgtgcatatc cgagcattcc gccaatcagg ttgttgaatt catgggcgat
     6901 gccggcggcc agctggccaa ggcccgccag tttctccacc tgggtgatct gctcctggag
     6961 cgaacggaga cggatcaaca cgctgatccg ggccaggagt tcacgggttt tgaccggttt
     7021 gacgaggtag tcatccgccc cgatctcgag cccgagaatc ttctcgtcga ttgacgagcg
     7081 ccctgacagg atgatgacgg gaatggcgcg caagcgggtg tctgtcttga tccgtttgca
     7141 gacttcatac ccgtcgatcc cctcgagcat gatgtcgagg aggatcagat ccggtctgag
     7201 atcgtcctga agtcgttcga gagccttttc acccgaggcg atccgggagc agaggtagcc
     7261 ggcatcctcc agcagcatca tcatgaagga tgcgtggctg atatcgtcat cgatgatcag
     7321 gatatgccgt ttcattccgg actctcttct cccatattgg actggtggtg cgttccggta
     7381 aagatagcac atcccgtcag ttatagtttt tccaacctcg ttgatacgcc tggtagatgc
     7441 gcggtgtgtt gagggtgtta atgatccggt tcttcggtct ggtcgaatcg ggagcgaatc
     7501 cgagtgcatt ggaaaaaact gccggagtga tgtggcgcaa tcgtgcggga agccgggtcc
     7561 agtcgggttg ccagcggatg gcggacgcca tcacgaatcc ccagtcgccg aacgagggaa
     7621 cgttctcatg gtaggggaca gtgtgcggaa agacttccga cagggttgcg tggatcatcc
     7681 agaaaacctc cctggcaaaa aacggagagg ccgcctgggt gacaaagacc ccggcccgtg
     7741 ccagtcggtg gcgcacgagg cgatagattt ccctcgaata gagtttggag aggacttcgt
     7801 tgtgcggatc tggaaagtcg gcgacgatca ggtcgtacaa tccggtcccg gtttcttcga
     7861 gaaatttgaa cgcgtccttg ttgaagatcc gcacccgggg gtctgccagg gcattgctgt
     7921 tggccgcaac cagggccgga tgggtctggg ccaaccgggt gatggccgga tcaagatcca
     7981 cgagatggat ctccttgact tcaggccgct tgaggatttc ccggacggcc atgccatcac
     8041 caccacccat caccagagcc cgctcgatcc cggggagggc cgtcatggca ggctggacca
     8101 gactctcgtg gtagcgatac tcgtccacgg tcgagaattg cagactcccg tccagccaga
     8161 gacgggtgtc gtcgcgccaa ccggtcaaca cgattttttg gaactgcgat tgctcgctgt
     8221 gcatgactgt gtcctggtag agttgctgtt cgtaccaggc cgccagggga tcggacgcaa
     8281 aaaacacgct gcagagggcc agcaatcctc ccagtgttgc cccgatccgg aggggccggg
     8341 caatccgttc acggaagaca ttcagcagga caaggaccac cagaatattg aaaattccgg
     8401 tgacgaaggc ggtccggatc atgccgaaga cgggaaggag aatgaaaggg aagaggaggg
     8461 aagcggccag cccgccaaca tagtcaaagg tcaagaccgt cgagagggaa ctggagagag
     8521 gcccgtaact tcgcatgatg cgtgtgacga gtggaatctc gaagccgatc agtgttccca
     8581 tggaggctga gagaagaatg aagaccggca tgtagagatc gccgagcaga tagacggagt
     8641 agaggatgat cagggagagt cccccgagca ggcccagtcc gagttccaca tccgcaaaga
     8701 tcgcgaccag gtccccggtc agccagcgcg agagccagga accgagaccc atggccgaga
     8761 ggaagatccc gatcgtcagg gaatactgga caacggaatt tccggtgagg tacgaggaga
     8821 cggcgccgat cagaagttca taggtgatcg cgcagacact tgtcaggagg atcgaaaaaa
     8881 gcagggcgcg aacctcgccc tgcttttttc tgtcgagcgg ctctgtcact tgctcttgtc
     8941 atccttggcc ggtgcgggtt tggcagcagg ggcttcggct ttgtagatct tggtttccgt
     9001 gcggatgacc ttgccggctg aatcatagct cttggtttcg atccgctcga tgctcccgct
     9061 gacctggaga tttgactcga ccttgttttc aaaggagaag atggcagcgg cgatgatgat
     9121 cgagatcccg atgatgacgg ccgctatgat cagtcccagg gacgtgttct ggtcgacttc
     9181 gatctctttc ttgatcgaga agggcgttac aacccgcacg atcaggaagc ccaggcccat
     9241 caggatgatg ccgagaatcc cgtaaatggc tgacgacagg atgccttcaa tgattttttc
     9301 catgatttcc tccggttgct gattggttga ttttgtactg catatccact tccgctgacg
     9361 ggtcccgcac cgagctgata cggttcacgt cctgctattt cccaaggcgt ggtccgccgc
     9421 cgtaatgcga gcggtagccc gttgtgcgat gggcggggct cccctttccg ggctggctca
     9481 accccggcgt tttccggata aattccgttc cgctccgggg gatgaagggt ttggcaatgg
     9541 tgccgtagag accgatacca ccgagggcga gcagcatgat ggcagttttt atcacacggg
     9601 tactcctttc tttgggtggt tctcaatccg ggagaagacc acccggtatc gagccggttg
     9661 tctttcgtct ggaacgggca cggagggcga ggaagaggga caggagtccg gcgacaagga
     9721 ggaaggagta actgcgttcc ccgtcacgga tcatgaccgc cacgtctgcg accgtccggt
     9781 tgcttttttc ggaggtgatg ctcaactcga tggtgtaggt cccattcttg tccagaacga
     9841 actcctcact gtcgttccct ccgctctcgc tccaataacc ctcgctgtcc gtccccgagg
     9901 catgccagat ccccgcggtg aaggaataga cgagttcgtt ggacgtgggt gaaacgaccc
     9961 gtgagagaac agcggttgac tcgatccatt cgttgtggat gctcggggct gccagccgga
    10021 gttgatggat cggcccggct tcttcg
//
LOCUS       DAOSHE010000020         9942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5794_length_9942_cov_4.044907, whole genome shotgun sequence.
ACCESSION   DAOSHE010000020 DAOSHE010000000
VERSION     DAOSHE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_9942_cov_4.044907"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLD82_01220"
     CDS             complement(<1..900)
                     /locus_tag="PLD82_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG64039.1"
                     /translation="MEGSARKNRLHIDWMIIFATIALVFIGLLTIYSTGSTHSGGKDV
                     DREFYMQFVWFVVGILLFFGTISISYNKYVEWSLYLYAGGLALLLISLLFPPVKGIRA
                     WINLGFFNIQPAELMKLFTIIALARFLNMLGDDIQDIRNFFITFFILVPPLGVIAIQP
                     DFGTGLVFFAICFAMLFIAGAKIGHILSLFLLGFLGAGIPMFVAYYKEIGDLSGWLIS
                     ILSNNAILIDISIILGIISFVAFAIWFSSRVKPVLKFASAIVVIPIGLIIAASLNSYL
                     QPYQRKRIVIFVDPEKDPWGAGYN"
     gene            complement(919..2772)
                     /gene="mrdA"
                     /locus_tag="PLD82_01225"
     CDS             complement(919..2772)
                     /gene="mrdA"
                     /locus_tag="PLD82_01225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG64040.1"
                     /translation="MQKYTIRPGQQRVFRQRVTTLFLLIAMMLLMLLLRIAYLQIIKV
                     GEYEARSEQNRVRLVPIMAYRGEIYDRNFKQKLIANRKSLCVTIVPAAMPKEKAEWDR
                     VLGRLASVLEMSTNELRRLMNKDKWDQYSPKLLKEGVDYQTITRIAENIDEYPGVYWD
                     NRPIRVYTLTNTLAHILGYTGYISREELKAASGRGYRPGALIGKTGIENVYDVDLRGE
                     EGTLEREVNALNQVTYQMVRKQPVHGRPLVLTIDASMQKVAQAAMGKMRGAVIVTKPA
                     TGEVLAILSNPGYAANLFYKRLDIESYRKIESDPDKPLFNRVIQAQYPASSTFKLITT
                     IAGLETGRIAPKTSYFCDGGYMLGDRYFKCWFGRHGHQALSDGIMNSCNVFFYRAGLE
                     IGPDSILHYARMFGFGKKTGIDLVGEVSGFVPTREWKRKTAGRPWLDGDTLNLAIGQG
                     DLLVTPVQINMMTCAIYNEGIAYQPYLKKEIRSLYNNHAIERNEGRHVLFTTDVSKST
                     FATLKAGMLRVVRGGTGSAAWSPVVQVAGKTGTAETSQDTTHAWFTGFAPADAKNPED
                     VIAVTVVCENAGGGGAMAAPVAACIIRSHFENRSVDETMQRIWKSYEQKPD"
     gene            complement(2776..3270)
                     /gene="mreD"
                     /locus_tag="PLD82_01230"
     CDS             complement(2776..3270)
                     /gene="mreD"
                     /locus_tag="PLD82_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG64041.1"
                     /translation="MRNTLIIALLIVFFGLLQSTVFVDILRIDEVKPDLLLIVIAFVA
                     FKRGGMAGQLTGFAAGMLQHFFSPALFGSYAFVFTVVGFVLGLTQHKVNAENFITSAV
                     LVFIATIIKGLALGILALLFVEIGDFYPSYVSGGLLLELVINPILAGPLFWMMNRFAA
                     PAMR"
     gene            complement(3267..4139)
                     /gene="mreC"
                     /locus_tag="PLD82_01235"
     CDS             complement(3267..4139)
                     /gene="mreC"
                     /locus_tag="PLD82_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG64042.1"
                     /translation="MLSVFFYKNRKVLAFLLLLIVSFTMMGTSSTSFTLNLKSIILTI
                     IYPFEYVINGIFNFFRSLWEGLGEIETLKKELLRTQARLKKYEELDSSIQTLQRRNAH
                     LERLLDERKQVRYEHVLASIVGKDPQNYYQSFIIDKGSSDGVAERMPVIAYENGKIGI
                     VGRVTMTTPFSALVTTIRGSRTWVGAILEASRYYGMVRGNGMSAHCLIEYVDVGAPAK
                     NGDVVVTSGHSDIYPRGLSIGILTNVQRDKGQFFLKAEVAPTINFAKLENVYVVKKLP
                     SPEIEKLEKEQKKL"
     gene            complement(4199..5224)
                     /locus_tag="PLD82_01240"
     CDS             complement(4199..5224)
                     /locus_tag="PLD82_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG64043.1"
                     /translation="MVLDKLYSLFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVEN
                     GTRRILAVGQEAKRMLGRTPGDIIAIRPMKNGVIADFETVQRMIKFFIQKVHSRKTLV
                     KPRIVIGIPSGITEVEKRAVKESAEQAGAREIYLIEESLAAAIGANIPINEPAGNMVI
                     DIGGGTTEVSVISLGGMVLSNSIRIGGDDFDEAIVQYLKKVHNLIIGERTAEEVKIQI
                     GNAYPASKKVETMEIKGRDSITGLPRTLEMDSVEIREALQEVINAVVEVVRSTLDQTP
                     PELAADIVDRGIVMTGGGSLLKGLPEHLSQETGVPVFLAENPLTCVVLGTGRYLEELK
                     YIQRGKY"
     gene            complement(5354..6361)
                     /locus_tag="PLD82_01245"
     CDS             complement(5354..6361)
                     /locus_tag="PLD82_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64044.1"
                     /translation="MKRSTIERLAKGGKDAGSQHILLKGAVIGIVLGLLLTVVLLVTR
                     TPDVYRNIEEMTEESRFEDAEKLARSLYEKDPLDVRALVLMGRNAFRKAFRFDNEKND
                     KTEEGWDLYKISVESLKKAILLDKKGLLTSADYFILGFAYLRKGDQYFEDALQYMKKA
                     EELNTNDHVLVQGNDPIARFDTLQQTIGYLNYHLGRYTNALQYYRDANAKPNVLNWAY
                     IGLCEVALGQYSNAIWSFGIVRDHANQQGLKAFATRQLAWTYFHLGRHDEARTWFEKT
                     VSIDTNYAEGYYWLGKMAELRNDLQAARALWQKSLEADPHFGPSILKLRFSPQHKTNL
                     K"
     gene            6527..7798
                     /locus_tag="PLD82_01250"
     CDS             6527..7798
                     /locus_tag="PLD82_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG64045.1"
                     /translation="MAKHASSAHSKQKVEWDLKRILSAIFVAAISGLLIVFFIASDAI
                     SSCSGSSGQNGRLLLAEYKGGRIYLGDRDLANTVDNMQEQYRSANSQELAREAVNRTG
                     QARIFSSAARRLGTAVTEETLKALLLATFSDPAQFNSADRSYQKEVLSQIETDFLRKA
                     LQTDIMASSRTTRLGLRIDSEIQAMKTAIDVVSLDLFTLIKNKTPSNEELKTWFESQK
                     GTRGEEVWRKATAFEAVTGEARTALIESWKKANAAALMMGAVKEMDDKLKIVDNAVMA
                     GQSLEAAAAAAGLPVLRTDFFAFGETPKAGETMVPVNRDFFRTLSGVATGSTSTVVKI
                     AEKDGIPQAMLLFRVRERRTIPDYDPALIADKTAFDKLPASEQEKIRELIRTQLKRIE
                     EQGKYALFNDFFTYLWMAANFTINEENLKKL"
     gene            7814..9610
                     /locus_tag="PLD82_01255"
     CDS             7814..9610
                     /locus_tag="PLD82_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOG64046.1"
                     /translation="MSNSDPDQGLSAGKTRYDRLFHFSLPETLPAEAVVTPAKTGRPT
                     LVVTKRHIHSAFDPEKEAVRLVTPHAAATAGILFGYGCGHLALAWLQQGLRPLLVVEC
                     RPDLLPAGRATAKDLPVDAPIFFARAWEKNLLEEDIRRFARSQPAAALKSLAWLDTPG
                     IVALDPPLWIQARSAVRGALTRHLTNLFTELEFERLWFSNILSNAALLPASERLADLA
                     GTQAGRTVMIIGAGPGLDRWLPWLAEAREHAVLLAVDTALKPLTAAGVIPHAVISLDG
                     QIHNLHDFTGIDTSEMTLFADISVHPAIPRLPFARRVFFETADIVETDGRPAIISHPL
                     TLWCKQAVGEIGAARSGGNVGTSALELARILGASKILFVGCDHAWPGGLTHAREAPAM
                     QRLRHKETRLMSASGVMRGAISRRAWIPGQDYHGRPVRSDIILSKYADWTVAAYREAV
                     REAQDAGRPMPDWATLSSEGLVLDGIPALCGPAEASAYIGTGRIPTSVFPVTGTGISR
                     QGAADLEARLRLLGEATSQLLTQARPLPPLEMVLDLFTRFPFLRRGYGAQVLHAEKTG
                     NAEWLLGEVRFQLTRIRHILVREGFCNRPVSH"
     gene            complement(9699..>9942)
                     /locus_tag="PLD82_01260"
     CDS             complement(9699..>9942)
                     /locus_tag="PLD82_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64047.1"
                     /translation="IAAMLARISPGGDFVWVCDYYPQIVDVLLLMQPWIASGEFVNAN
                     PGCGYGDGIEGYPRTLYESRWRAMGRRIYYVRCIRR"
BASE COUNT         2195 a         2934 c         2724 g         2089 t
ORIGIN      
        1 gttgtagccg gcaccccagg gatccttttc gggatcgaca aaaatcacga tccgctttcg
       61 ctggtatggc tggagatagg agttgagtga agcggcgata atcagaccga tcggaatcac
      121 gacaatggcc gatgcaaatt tcaataccgg tttgacccga ctggagaacc agatggcaaa
      181 cgcgacaaaa gaaatgatcc cgaggatgat ggagatgtcg atcaatatcg cattattgga
      241 cagaatggaa atcagccagc cggagaggtc accgatctcc ttgtaatacg caacgaacat
      301 cgggataccc gcaccgagaa acccgagcag gaacagggag aggatgtgac cgatttttgc
      361 accggcaatg aagagcatgg caaagcaaat ggcaaaaaaa accaggcccg tcccgaagtc
      421 aggctgaatg gcaatcacgc ccagtggcgg cacaagaatg aaaaaggtga taaagaaatt
      481 cctgatgtcc tggatatcat caccaagcat gttgagaaac cgtgccagag caatgatggt
      541 aaagagcttc atcagttccg caggctggat gttgaagaaa cccagattga tccacgcccg
      601 aatgcccttg acaggaggaa agagcagtga aatcaagagc agggcaagcc cgccggcgta
      661 gagatagagc gaccattcaa cgtacttgtt gtagctgatg gaaatcgttc cgaaaaaaag
      721 caggattccc accacaaacc agacaaactg catataaaat tcccggtcaa catccttccc
      781 tccgctgtgg gtggagccag tactgtagat ggtcaacaag ccgataaaga caagggcaat
      841 cgtcgcaaag atgatcatcc agtcgatgtg cagacggttt tttcgtgccg aaccctccat
      901 caggcaggcc tcccttgttc agtcaggttt ttgctcataa ctcttccaga tacgttgcat
      961 ggtttcatcg acagacctgt tttcaaaatg ggagcggatg atgcaagccg caaccggtgc
     1021 cgccatggca ccgcctccgc ctgcgttttc gcagacaacc gtaacagcta tcacatcctc
     1081 gggatttttg gcatctgccg gagcaaaccc ggtaaaccag gcatgggttg tgtcttgtga
     1141 ggtttcggct gtcccggttt ttcccgcaac ctgaacgacc ggtgaccagg cggcacttcc
     1201 ggttccgccc cgcacaaccc tgagcatgcc tgccttgagc gtggcaaagg tggatttgga
     1261 gacatcggtt gtaaagagaa catgccgccc ctcgttccgc tcgatcgcgt gattgttgta
     1321 gaggctccgg atttcctttt tcaggtaggg ctggtaggcg atgccctcat tatagattgc
     1381 acaggtcatc atgttgatct ggaccggagt gacgaggagg tcaccctgac cgatggcgag
     1441 attgagcgtg tctccatcaa gccatggccg ccctgcggtt ttccgcttcc actccctagt
     1501 cgggacaaac cctgaaactt cacccaccag atcgatcccc gtttttttgc caaacccgaa
     1561 cattctggcg taatgcagaa tgctgtcggg tccgatttca agaccggcac gataaaaaaa
     1621 gacgttgcag gaattcatga tcccgtcgga gagtgcctgg tgcccatgtc ggccgaacca
     1681 gcatttgaaa taccggtccc cgagcatgta cccgccatca cagaaatagg atgttttcgg
     1741 tgcaatccgt ccggtttcga gtccggcgat ggttgtgatc aacttgaacg tcgaactcgc
     1801 gggatactgt gcctggatca cccggttgaa gagcggcttg tccggatcac tctcgatctt
     1861 gcggtaggac tcgatatcga gtcgcttata gaagaggttg gccgcatacc ccggattgga
     1921 aagaattgca agaacctctc cagtcgccgg ttttgtcaca atgaccgcgc cacgcatctt
     1981 tcccatggcc gcctgagcga ctttctgcat ggatgcgtcg atcgtcaaaa ccagcgggcg
     2041 accatggacc ggctgtttcc ggaccatctg gtaggtgacc tggttgaggg cattcacctc
     2101 acgttcgagg gtcccctcct cgccacggag atccacatcg tagacattct cgattccggt
     2161 ttttccgatc agggccccgg gacggtatcc ccttcccgag gcggctttga gttcctctcg
     2221 actgatgtat ccggtgtacc cgagaatatg tgccagggta ttggtcaggg tatagacccg
     2281 aatcggcctg ttatcccagt acacaccggg gtattcgtca atattctccg caatcctggt
     2341 gatggtctgg taatcaacgc cttccttgag gagtttcggg ctgtactggt cccatttatc
     2401 cttgttcatc aaacggcgaa gctcgttggt ggacatctcg aggaccgagg ccagcctgcc
     2461 aagcacgcga tcccactcgg ccttctcctt gggcatggct gccgggacaa tggtcacaca
     2521 gagggacttg cggttggcaa tcagcttttg tttgaaattt cggtcataaa tttctccgcg
     2581 atacgccatg atcggaacaa gccgcacccg gttctgctcg gagcgcgctt catactcacc
     2641 gactttgatg atctggagat aggcgatcct gagaaggagc atcaggagca tcatggcgat
     2701 caggagaaac agcgttgtta cacgctgtcg gaaaacacgt tgctggcccg gccggatcgt
     2761 atacttctgc atggctcacc gcatcgccgg cgctgcaaac cggttcatca tccagaagag
     2821 ggggcccgca agaatcgggt tgatcaccaa ctccagaaga agcccgccgc tgacatagct
     2881 cgggtaaaaa tcaccgattt cgacaaacag cagggcgaga atcccgaggg cgagtccttt
     2941 gatgattgtc gcaatgaaaa ccagaacggc cgaggtgatg aagttctcgg cattcacctt
     3001 gtgctgtgtc aatccgagga caaacccgac aacagtaaaa acgaaggcgt aactcccgaa
     3061 aagggccggc gaaaaaaaat gctggagcat gccggcggcg aagcccgtca actgacccgc
     3121 catgccgccc cgcttgaagg ccacaaaggc aatcacgatc aaaagaagat ccggcttgac
     3181 ctcgtcaatc ctgaggatgt ccacaaagac ggtgctctgc agcagcccga aaaacacgat
     3241 caggagagcg atgatgagag tgtttctcac agttttttct gctccttttc cagtttttcg
     3301 atttcaggcg acggcagctt tttcacgaca tagacgtttt caagcttggc aaaattgatc
     3361 gtcggggcga cttcggcctt gaggaagaac tggcccttgt cccgctggac attcgtcaga
     3421 atcccgatgg agagcccgcg gggatagata tcgctatgac ccgatgttac aacaacatca
     3481 ccgtttttcg caggcgcccc gacatccacg tactcgatca gacagtgggc cgacattccg
     3541 ttcccgcgca ccatcccgta atagcgggaa gcctcaagga tggcgccaac ccaggtccgt
     3601 gacccgcgta tggtcgtgac cagtgccgag aacggcgtcg tcatcgtgac acggccgaca
     3661 ataccgatct tcccgttttc gtatgcgatg accggcatcc gctcggccac accgtcagac
     3721 gagcctttgt cgatgatgaa cgactggtag taattttgcg ggtccttgcc gacgatcgac
     3781 gcaagcacgt gttcgtagcg tacctgcttg cgttcatcaa gaagccgctc aaggtgggca
     3841 ttccgtctct gcagtgtctg gatggaactg tcaagttcct catatttttt cagccgcgcc
     3901 tgggtccgca ggagttcctt tttgagagtc tcgatctcgc ccaaaccttc ccagagggaa
     3961 cggaaaaaat tgaagatccc gttaatcaca tattcgaacg gatagatgat tgtgagaatg
     4021 atgctcttga gattgagtgt gaaactcgtg ctggacgttc ccatcatcgt gaacgagacg
     4081 atcaggagaa gcaggaaggc cagcaccttg cgatttttat agaagaaaac tgaaagcatt
     4141 ccggaatctt tccacccacg ttcatctggt ttggcacgtc agttcgggga aaccctgatc
     4201 agtactttcc acgctggata tacttgagtt cttcgagata gcgcccggtt ccgagaacaa
     4261 cgcaggtcaa cggattttcg gccaggaaca cgggaacccc ggtctcctgc gaaaggtgct
     4321 ccggcaatcc cttgaggagc gaaccaccac ccgtcatcac gattccgcga tcgacaatat
     4381 cggccgccag ctcgggaggg gtttgatcga gtgtactccg gacgacttcg acaacggcat
     4441 tgatcacttc ctggagtgcc tcacggattt cgacggaatc catctcgagc gtccggggca
     4501 ggccggtgat cgaatcacga cccttgatct ccatcgtctc gacttttttg gaggcaggat
     4561 aggcgttccc gatctggatc ttgacctctt cggcagtccg ctctccgatg atcaggttgt
     4621 gcactttttt gaggtactgc acgattgcct catcaaaatc atcgccaccg atccggatcg
     4681 agttggagag caccatgcca cccagcgaga tcaccgaaac ctcagtcgta ccgccaccga
     4741 tatcgatcac catgtttccg gccggctcgt tgatcgggat gttcgctccg attgccgcag
     4801 ccagggactc ttcaatcagg tagatctcgc gggctcccgc ctgttccgct gactccttga
     4861 ccgcccgctt ctccacttcg gtgatccccg aaggaatccc gatcacgatg cggggcttga
     4921 ccagggtctt ccgcgaatgg accttctgga tgaagaactt gatcatgcgc tggaccgtct
     4981 cgaaatcggc gatcacaccg tttttcatcg gacgaatggc gatgatatcg cccggggtcc
     5041 gcccgagcat gcgctttgcc tcctgcccga ccgccagaat ccggcgggtg ccattttcga
     5101 cggcaaccac ggagggctcc gagaggacaa tgccctgccc cttaacgtga acaagcgtgt
     5161 tcgctgttcc gagatcaata cccatgtcat tcgaaaagag gctgtagagc ttatcaagta
     5221 ccatgtccgg tgaacccttt catcgcagaa gagtgagagc aggaattcgc ttcattgata
     5281 atatagatac ggccaccggc tctgcaaaca tttttcagtc cgctccggcc atgtttcggg
     5341 ccagcaggag tggttatttg agattggtct tgtgctgtgg agaaaatctc agtttgagga
     5401 tggacgggcc gaagtgcggg tccgcctcga ggctcttctg ccagagcgcc cgggcagcct
     5461 ggaggtcgtt tcgcagttcg gccatcttcc ccagccagta gtacccttca gcgtaattgg
     5521 tgtcgatgct gacggttttc tcgaaccacg tccgggcctc atcgtgcctt ccgaggtgga
     5581 aataggtcca ggcgagctgc ctcgtggcaa aggccttgag cccctgctgg ttggcatggt
     5641 cccgcacgat accgaaggac cagatcgcgt ttgaatactg gccgagcgcg acctcgcaga
     5701 gaccgatgta ggcccagttc aggacgttcg gctttgcgtt ggcatctcgg taatactgca
     5761 gggcgttggt gtatctgccc aagtggtaat tgagatagcc gatcgtctgc tggagggtgt
     5821 cgaaccgggc gatcggatca tttccctgca caagcacgtg gtcattggta ttgagttcct
     5881 cggccttctt catgtactgc agagcgtctt cgaaatactg gtcgcccttg cggaggtagg
     5941 caaaaccgag aatgaaatag tccgccgatg tcaacaggcc ttttttatcg aggagaatcg
     6001 ccttcttgag ggattccacg gagattttgt agagatccca cccctcctcg gtcttgtcgt
     6061 ttttctcgtt gtcaaaccgg aaggccttgc ggaaagcgtt tcgccccatc aaaaccaggg
     6121 cccggacgtc aagcggatcc ttttcgtaca gtgaacgagc cagtttctcg gcatcttcaa
     6181 accggctttc ctcggtcatc tcctcgatat tccggtagac atccggcgtc ctcgtgacga
     6241 gcaggacaac cgtgagcagg agcccgagaa cgatcccgat gaccgcgccc ttgagcagaa
     6301 tgtgctggga accggcgtct tttccgccct ttgccagtcg ttcgatggtt gatcgcttca
     6361 tcctgccctc cagcgtgcaa tttactcctg ttgaccggcg ttgtgtgcat tttcaacgga
     6421 ttccgaaacg agaacagtct ttatttttcg tttctcccgt gctagattgg tactgcacgt
     6481 ccttccggac gcgactttct acaaaccgca ggagtatccc ccagcgatgg ccaagcacgc
     6541 atcctccgct cactcgaaac aaaaggtcga atgggacctc aaacgcattc tctcggcaat
     6601 ttttgtcgca gcaatcagtg gactcctgat cgtcttcttc atcgcgtcgg acgcgatctc
     6661 cagttgcagc gggagcagtg gccagaatgg ccgactcctt ctcgccgaat acaagggcgg
     6721 acggatttac ctcggagaca gggatctggc gaatacggtc gacaatatgc aggagcagta
     6781 ccgttccgcc aattcccagg agctcgcccg tgaagcagtc aaccggacgg ggcaagccag
     6841 gattttctcc agtgcggccc gccggctggg cacagccgtg acggaggaaa cgctcaaggc
     6901 tctcctcctg gcgaccttct ccgaccctgc ccagttcaat tccgcggatc gcagttacca
     6961 gaaggaagtc ctgagccaga tcgaaaccga ttttcttcga aaagcgctgc agaccgacat
     7021 catggcctcc tcccgcacga cccggctcgg gctgcggatc gacagcgaga tccaggcgat
     7081 gaaaacggcc atcgacgtcg tctccctcga ccttttcacc ctcatcaaga acaagacgcc
     7141 ctcgaacgag gaactcaaga cctggtttga aagccagaag gggacccgcg gcgaagaagt
     7201 ctggcgcaag gccaccgctt ttgaggctgt caccggtgaa gcccggaccg cactgatcga
     7261 gtcgtggaaa aaggccaacg ccgcagctct gatgatgggt gccgtcaaag agatggacga
     7321 caaactgaag atcgtcgaca acgcggtcat ggccggacag tcccttgagg ccgcagccgc
     7381 agcagcggga ctccccgttc tccggacaga ttttttcgcc ttcggcgaga cacccaaagc
     7441 cggcgagacc atggttcccg tcaaccggga tttcttccgc actctgtccg gtgtggcgac
     7501 cgggagcacc tcgacggtgg tcaagatcgc cgagaaggac gggattccac aggccatgct
     7561 cctcttcagg gtccgcgaac gcaggaccat cccggactac gacccggccc tgatcgccga
     7621 caaaaccgcc ttcgacaaac tcccggccag cgagcaggaa aagatccgcg agctgatccg
     7681 gacccagctc aagcgcatcg aggaacaggg gaaatacgcc ctcttcaatg acttcttcac
     7741 ctacctttgg atggctgcga atttcaccat caacgaagaa aacctgaaaa agctgtaaga
     7801 gcagacagga tcaatgagca attccgatcc ggatcagggc ctgtcggcgg ggaagacccg
     7861 ctatgacagg ctcttccatt tttccctccc tgaaacactt ccggccgagg ccgtcgtcac
     7921 accggccaag accggccgcc cgaccctcgt cgtcacaaag cgccacatcc acagcgcctt
     7981 tgatcccgaa aaagaagctg tgcgcttggt caccccccac gccgcggcca cggccggaat
     8041 cctcttcggg tacggctgcg gccacctggc cctggcctgg cttcaacagg gtctgcgccc
     8101 ccttttggtt gtcgaatgtc ggcccgacct tctcccggcc ggccgcgcca ccgccaaaga
     8161 tctcccggtg gatgccccga ttttttttgc ccgggcctgg gagaagaatc ttctcgagga
     8221 agatatccgc cgctttgccc gcagccagcc ggcggctgcc ctgaaatctc tggcctggct
     8281 ggacactcct gggatcgtcg cacttgatcc acctctctgg atacaggcca ggtccgccgt
     8341 ccggggagca ctgacgcggc atctgacaaa cctctttacc gaactggagt tcgagcgact
     8401 ctggttctcc aacatcctct ccaacgcagc gctccttccc gcgagcgaac ggctggctga
     8461 tctggccgga acgcaagcgg gacggaccgt tatgatcatc ggagccggtc ccggtcttga
     8521 ccgctggctc ccctggctgg ccgaagccag ggagcatgcc gtcctgctag ccgtcgatac
     8581 agccctcaaa cccctgacag cggccggggt catcccgcac gccgtcatca gccttgatgg
     8641 acagatccac aacctccacg acttcacggg catcgacacc agtgagatga ccctctttgc
     8701 ggatatcagt gtgcatcccg ccatcccccg tctccccttt gcccgccgtg ttttttttga
     8761 aaccgcggac atcgtcgaga cggatggccg gccggccatc atcagccacc ccctgacact
     8821 ctggtgcaag caggctgtcg gagagatcgg tgctgcccga agcggcggaa atgtcggaac
     8881 aagtgccctt gaactggccc ggattctggg cgcctcgaaa atcctgtttg tcggctgcga
     8941 ccatgcctgg ccgggtggcc tgacccacgc acgagaggcc ccggcaatgc aacgcctgcg
     9001 ccacaaggaa acgagactca tgtccgccag tggggtgatg cggggtgcca tctcgcgtcg
     9061 agcctggatc ccgggacagg attatcacgg acggcctgtc cgcagcgaca tcatcctgtc
     9121 aaagtatgcc gattggaccg tggccgccta ccgggaggcg gtccgtgaag cacaggatgc
     9181 agggagaccc atgccggact gggccaccct ctcgtccgaa ggtctggtcc tggacggaat
     9241 cccggcactg tgcggtccgg ccgaagcatc ggcctacatt ggaaccggac gcatccccac
     9301 ttcggtcttt cctgtgaccg gcaccgggat ttcccggcag ggagcggcgg atctcgaagc
     9361 ccgtctccgc cttttgggag aggccaccag ccagctcctg acccaggcca gaccgctccc
     9421 gcccctggag atggtgcttg atctgtttac gcgcttcccg tttctgcgcc ggggatatgg
     9481 cgcccaggtt cttcatgccg aaaaaaccgg aaacgcggag tggctcctcg gagaggtgcg
     9541 gttccagctg acccgcatca gacacatcct ggtccgcgag ggattttgca acaggccggt
     9601 cagccactga caggcgatgc tcgccgacgg gaacaggtgc atactgggtg tgcccatcgc
     9661 ccctcaacag gcggaagatc gttcctcaaa atgaaccttc agcgacggat acaccggaca
     9721 tagtagatcc gtcgccccat cgcacgccag cgggactcat acagggtacg gggatatcct
     9781 tcaataccgt ctccatagcc acatcccggg ttggcgttga caaactcgcc actggcaatc
     9841 cagggttgca tcaggagaag cacgtccacg atctgggggt agtagtcgca gacccagaca
     9901 aaatcccccc cgggagatat ccgggcgagc atggctgcaa tc
//
LOCUS       DAOSHE010000021         9886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5838_length_9886_cov_3.642559, whole genome shotgun sequence.
ACCESSION   DAOSHE010000021 DAOSHE010000000
VERSION     DAOSHE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_9886_cov_3.642559"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /gene="groL"
                     /locus_tag="PLD82_01265"
     CDS             complement(<1..1168)
                     /gene="groL"
                     /locus_tag="PLD82_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751305.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG64048.1"
                     /translation="MAKQMLFNEEARRAMLSGVNKLADAVRVTLGPKGRNVVIEKKFG
                     APVITNDGVTIAKEIDLEDPYENMGAQLVKEVSTKTNDMAGDGTTTATILAHAIFSEG
                     LKNVTAGANPMKLKRGIEKAVHFCVDAISKLSEPVKANKQKIAQVATVSANNDDEIGN
                     LVADAMEKIQYDKDGVITVEEAKGIETTLQTVEGMQFDRGYLSPYMVTDPESMEAKLE
                     NAFILIHEKKISSMKDLLPLLEQVVKQGAPLLIIAEDVEGEALATLVLNKLRGTLNCV
                     AVKAPGFGDRRKAMLEDIAVLTGGTMICEDLGMKLESATLNDLGRAKIIKINKENTTI
                     IEGHGEEKKIQERIKQIKAQIEETTSDYDREKLQERLAKMSGGVAVLSIGAATEV"
     gene            complement(1731..1803)
                     /locus_tag="PLD82_01270"
     tRNA            complement(1731..1803)
                     /locus_tag="PLD82_01270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1768..1770),aa:Thr,seq:cgt)
     gene            complement(1835..1907)
                     /locus_tag="PLD82_01275"
     tRNA            complement(1835..1907)
                     /locus_tag="PLD82_01275"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1872..1874),aa:Phe,seq:gaa)
     gene            2172..2444
                     /locus_tag="PLD82_01280"
     CDS             2172..2444
                     /locus_tag="PLD82_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOG64049.1"
                     /translation="MPYDITGSVIRIMDTQTFPSGFSKREFVIQTDEKYPQQVKLECI
                     KDRCSLLDGIRENDQVSVRFAVRGSEHEGRYYVNLQALEINRQNPF"
     gene            2444..3862
                     /locus_tag="PLD82_01285"
     CDS             2444..3862
                     /locus_tag="PLD82_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG64050.1"
                     /translation="MEVPRTGRPVLTMLAVMGGVFLTAFLGSSLNIAIPDIENEFSVP
                     ASLIGWVATAYILTNAAFLLIAGWFGDRYGRILIYLVGLGITTGTTALAILSKDVTTL
                     IIIRAVQGVGSALVFANSVAIVSDAFPPERRGRALGLTIAATYLGLSLGPVIGGALIH
                     ESGWRSVFLPPLLIGLLSLFASWRILSAQPAHIPHRTSRVDCAGSLLSILAPVLFVSG
                     ISILGRNPTGGILAILAGILTLFIFILVERARPGWRLMDISLFSESRLFSFSCLAALL
                     NYSATFGGGFLLSVWLQYTRGLAPHEAGLILVIQPAIQALLSPLFGHISDRVDKRLLA
                     SAGMALTALAIFWFAITTLDDGLTGIMAGLSLSGTGFALFSSPNSNAIMSAVSPPQYG
                     AASSILALARTAGQLFSMGLVTAGFTLGFGSVKLGDLHPTELSASISLLFTIMGALTV
                     PGILFSLLRNKGKVQSSPRGNV"
     gene            3866..5596
                     /locus_tag="PLD82_01290"
     CDS             3866..5596
                     /locus_tag="PLD82_01290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG64051.1"
                     /translation="MGIMKSTASGMAGQHFVLTPLGGGREIGGNAWHIEWDDLSFLID
                     CGTHPERPAWLGLPALDRIARLDFVILTHAHSDHSGALPALLRSHPVPVWMTDATSSL
                     MGPMMQDALKLQRRFFDIDRFGRSARPELGPPLFESGDVERVFELQKSLDGGFSHKGL
                     TVTPFCSGHILGGVGLVLEKNSKRVLVTSDISDIPKETSRALFLPQGNFDLVISEGTR
                     GLDCGDTDLPLREDEEEQAIRILSRVLSRKGSVLFPAFVIERAQSVARLVMRARDSGR
                     IPPGVPLYMAGLADSMTALHAQALGLRLDWKPVPSRDWFDDLLSREQAILVAGSGMLN
                     SGSGAARLAEQLLSDRKREHGIIFTGYLTPQSPGGRLMHRERSPLSGHTHACINQRIV
                     DVRTNEIHQVHLSCHASGDSLVDLVRGMEPAGVILVHGDPDALATLSAAMRLAGLTDI
                     RVPENGESISFSGNPAATLIPPLDPSAEPMTVVERNPWNGRALRALAGENAILPDGES
                     ARNRLQSITNLLLERAMRDGNLGRAKSILDESGSFIPGDDREAITLRIDEIAAWCPQN
                     DDRTPNQRRD"
     gene            complement(5691..6866)
                     /locus_tag="PLD82_01295"
     CDS             complement(5691..6866)
                     /locus_tag="PLD82_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64052.1"
                     /translation="MKNLCKGIALAVLLTAVAVPGSAYFEDIGRDARARGMANAMIGI
                     FGNVNSVQYNPAGTARSTSLELNLQWGKPLTMGDLNDGSSFDAMNGTFLMPFTNDVNL
                     SWLIRWLFMGLTLGNEEFMFKDGAMALAFDMRDNAQLVKETMITLNYAKVLDDVLFKG
                     AKLAAGFNIDMYSLAFTKTADWGTLPDNPNDSVFTIGIDLGVIYNFSENIRLAVVFEN
                     LLKPNFSVFDGGEDYSPTNVKLGGSIFFPKLIFFENMTVTFNYVTYGKYDSSDNTATK
                     ASYHFGFESWWFDNIFAFRGGLQLGDEDMSELSLGISAVLPMGEHLVSFDYAFTLPFG
                     VEGTRHLLALTWKWDLPKYVFEYDPKKAAEMKRLHELEQQRLADEENKKNQTTATNK"
     gene            complement(6885..>9886)
                     /locus_tag="PLD82_01300"
     CDS             complement(6885..>9886)
                     /locus_tag="PLD82_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64053.1"
                     /translation="GIGITSARGATITLFSNYFSLFYPTPIAPKGGDQLALTIRDGFA
                     QGNTSTSLGVNVKFDTMAYFTNAAVANGTNRIEFVMPEPLVEFGLPVRDIYTTHTNAT
                     LRVWVTNRGSGSNLLERFVLTVPGVMSNVMTQVLAAVSTNVTHAPSGLVDARYLQGVR
                     PGQGDVFSIALVNPQHEVGSFTLNAIVSNSTLGDPALVKGVSNILRVVTPPQTRISPN
                     AIDTTTGTNTVEVHINNTGSGDTAVLRARVKIPEVFNRIVSVASTRVSGAAITNLGNE
                     YFVEYGPAAIANGSSDTLTFVLEDGLSHGATNVAWQVQVDNGNGYAMTAESTTGAMAQ
                     AFTMPAVAAAGWLNTVWVYAANDPGSVVTNVMTLSFTNRGRGSNNVYRVLLQVPQALS
                     NMSGLMSDRGGVVTVTNSTNVFVSYEGAGFLRPGQKDTLRFTLRHSVNNALSFDYIFK
                     ADNDDGNGYVAVTAPTAIAGAQQRLDVFIKEEDPEAYIVGAKAPYIWETVTNTVIYTM
                     DDAATVRYAIKNKSRRFSVNSVVIPLSNTFTVEGITSTLATGSASVAYTHATNTLILR
                     WVGTNFGQQMSDTLTLALSYDKNAFKAMIEASTKSLTNTVTARVRYGGSEQYILARGL
                     QRDKLVVENARFGRIRGVVLPLGLGPTVTLKDTASESIATNVEGKAFQALVNTNTGAY
                     VVDKIPPSLAYRLVVMPPIGETVTNNGRVIVPVNYDTLQVSGVSAVQNTVLQLTNLIM
                     VYGKLNKQATGDQQVICPEDALTKLVVPAGTLFEGISVNIAIEDMTDDQESYANASER
                     VITRPTDSTTLSVWHFKIVNLADNVTLENGFKKDVEIVLHYGDLSSQGWTDDRLGIWY
                     WKPSTKEWVFVGGVVDTQTKTIRARVSYLHTYYAVMATATATTQAIHSVKASPNPFTP
                     GRGDGQFSNMKLSFAFAKAYSSYEVKIFNMQGQLVQHFTRDGSYAQGEVFWDGKDRNG
                     FKVRGGVYVYQIIAGSSVYSGSVLILR"
BASE COUNT         2025 a         2947 c         2650 g         2264 t
ORIGIN      
        1 cgacctcggt tgcggcacca atactcagga cggccacacc accggacatc ttggccaagc
       61 gctcctggag cttttcgcgg tcataatcgg aggttgtttc ctcgatctgg gccttgatct
      121 gcttgatgcg ttcctggatc ttcttctctt cgccgtgccc ttcgatgatg gtcgtgtttt
      181 ccttgttgat cttgatgatt ttggcacgac cgagatcatt gagcgtcgcg ctctcgagtt
      241 tcatcccgag atcttcgcag atcatcgttc caccggtcag cacggcaata tcctcgagca
      301 tggccttgcg cctgtcgcca aagccggggg ccttgaccgc aacgcagttg agtgttccgc
      361 ggagtttgtt gagcacgagt gtggccagtg cctcaccctc gacatcctcg gcaatgatca
      421 ggaggggcgc accctgcttg acaacctgct cgagaagggg aaggaggtcc ttcatcgaac
      481 tgattttctt ttcatggatc agaatgaacg cgttttcgag cttggcttcc atgctctcag
      541 gatccgttac catgtagggt gaaaggtacc cgcggtcaaa ctgcatgcct tcgacggtct
      601 gcagggtggt ttcgatgccc ttggcctctt cgaccgtgat caccccgtcc ttgtcgtact
      661 ggatcttctc catagcatcg gccacgagat tcccgatctc gtcgtcatta ttggcggaaa
      721 cggtggcaac ctgggcgatc ttctgcttgt tggccttgac cggctcggag agcttggaga
      781 tcgcgtcaac gcagaaatga accgccttct cgatcccgcg cttgagcttc atggggttgg
      841 caccggccgt cacgtttttg agaccttcgg aaaaaatggc gtgtgccagg atggtcgcag
      901 tggttgtccc gtcaccagcc atgtcgttgg tctttgtcga gacttccttg acaagctggg
      961 cgcccatatt ctcgtatgga tcctcgaggt cgatttcctt ggcgatggtg acaccgtcat
     1021 tggtgataac cggtgcgccg aactttttct cgatgacaac attgcgaccc ttgggtccca
     1081 gagtgacccg caccgcgtct gcgagcttgt tgacaccgct caacatggcc ctgcgggctt
     1141 cttcattgaa taacatctgc ttcgccatag tacacctctt tttcgtatgg attgagcatg
     1201 gtaaacattt gtttataaac aaagtacgca aagtgaccgg gagaggcaat gaaaaaatgc
     1261 accaagagtc gcaaggtgga ttttttggag atgtcgaagc tcaaagaacg ccaagctgga
     1321 ttgtttccga aaaccacggc acagttctcg aagaggagtg ggtgtatgcg gggtggacaa
     1381 ctccgtctcc cttcgcgttg ctcccacgat ctgtcaaaga tgcgcctgtg gtgcgttgcc
     1441 gggagccgca acataaaaac gtgatattct catggatgtc ccggagtcgc ataacagtat
     1501 tgcgttttaa ttatacgaat ccccaaaaaa tgtgtcaaaa cgatacaatt ttgttcgatt
     1561 tgcaatcccg gattaccgtg catgggcgta gtcctcgaag aggggacttc gcgtgtatta
     1621 gccccggcaa atacgcaaac atcatgtttg ctgccgggac aatacatccg tgtattagcc
     1681 cctcgctatt gtccatcctg gacgctcggg acaaagcctt ccatggcttt agccctctgc
     1741 cagaatcgaa ctggcgacct acgcattacg agtgcgttgc tctaccggct gagctaagag
     1801 ggcaaatgaa gaaagggaat taaggaagac attgtgccga gagacggaat tgaaccgccg
     1861 acacggggat tttcagtccc ctgctctacc aactgagcta tctcggctcg tttgtgtaca
     1921 tacaaggtat gaaaaacaac gcaacgtggc aagaaaaagt caaaatttct gcacgtcagc
     1981 cagtcttttt ttggtgaaca acgtccactc gatggcatcc gtcggtgcca gaacgggcgg
     2041 actgtaccac gacagaccgc ccatgggatg atcagaggct gttcatcaaa aaaaatgacc
     2101 cgatttcaat tgacatcggg gaaggcgtcg ttgcatactt tctgcatcat tctcgactta
     2161 ggaaaggccc aatgccgtac gatataacag ggtctgtgat tcgcatcatg gatacgcaga
     2221 catttcccag cggtttcagc aagcgtgagt tcgtcatcca gacggacgag aaatacccgc
     2281 agcaagtcaa actggagtgc atcaaggatc gttgcagtct gctcgatggc atccgcgaaa
     2341 atgaccaggt cagtgtccgt tttgccgtcc gcggaagcga gcacgaaggt cgctactacg
     2401 tcaatctcca agccctcgag atcaatcgcc agaatccgtt ctgatggaag tacctcgtac
     2461 aggacgaccc gtcctgacca tgctggccgt catggggggc gtttttttga cggccttcct
     2521 cggttcttcc ctcaatattg ccattcctga catcgagaac gaattttcag ttccggcctc
     2581 cctgattggc tgggtcgcga ccgcctacat cctcacgaat gccgctttcc tgctgatcgc
     2641 cggttggttt ggtgaccggt acggccggat cctgatctac ctggttggcc tgggaatcac
     2701 cacgggaacc accgccctgg ccatcctctc caaggatgtc accaccctga tcatcatccg
     2761 ggccgtccag ggagtcggtt cggctctggt ctttgccaac tcggttgcca ttgtcagtga
     2821 cgcattccct ccagaacggc gcggccgggc cctcggactg acgatcgccg ccacctatct
     2881 cgggctctca cttggcccgg tcatcggggg cgcactgatc cacgaatccg gctggcgttc
     2941 cgtctttctt ccacccctcc tgatcggcct gctttccctg ttcgcatcct ggcggatttt
     3001 gtctgcacaa ccggcccaca tcccgcatcg gaccagccgg gttgactgtg ccggatctct
     3061 cctctcgatt ctggctcccg tcctcttcgt cagcggaatc agcatccttg gccggaatcc
     3121 cacgggggga atcctcgcca ttctggccgg tatcctgacc ctcttcattt tcattctggt
     3181 tgaacgggcc cgccccggct ggcgcctgat ggatatcagc ctcttctcgg agagccggct
     3241 cttctccttc tcctgtctgg cggccctcct caattacagc gcgacctttg gcggcggttt
     3301 tctcctgagt gtctggctgc aatacacccg tggtctcgct ccccatgagg ccggcctgat
     3361 tcttgtgatc cagccggcca tccaggccct cctctctccg cttttcggcc atatctccga
     3421 cagggtggac aaacgactcc tggcttcggc cgggatggcc ctgacagcgc tggccatctt
     3481 ctggtttgcc atcaccacgc ttgatgacgg gctgacgggc atcatggccg gcctgtcgct
     3541 ctccggcacc gggtttgccc tcttctcctc gcccaactcc aacgccatca tgtctgccgt
     3601 cagcccgcct cagtacggcg cggcctcctc gatcctggcc ctcgcccgga ccgccggcca
     3661 gctcttcagc atgggactgg tcacggccgg gtttaccctc ggtttcggca gcgtcaaact
     3721 cggtgatttg catcccaccg aactctccgc aagcatctcc ctgctcttta ccatcatggg
     3781 cgccctgacc gtcccgggaa tcctgttttc cctcctgcga aacaagggaa aagtgcaatc
     3841 cagtccccgc gggaatgtgt agtttatggg tatcatgaaa agcaccgctt caggcatggc
     3901 cggacaacac tttgtgctga ctcccttggg aggtggacgc gagatcggcg gcaacgcctg
     3961 gcatattgaa tgggatgatc tctccttttt gatcgactgc ggcacgcacc cggagcggcc
     4021 ggcctggctg ggtctgccgg cactggacag gatcgcccgg ctggattttg tcatcctgac
     4081 ccatgcccac agcgaccact ccggtgccct ccccgccctg ctccgctccc acccggtccc
     4141 cgtctggatg acggatgcga catccagtct gatgggtccg atgatgcagg acgccctcaa
     4201 actccagcga cgctttttcg atatcgaccg cttcgggaga tccgcccgcc ccgaactcgg
     4261 ccctccgctc ttcgagtccg gcgacgtcga gcgggtcttt gaactgcaga aatccctgga
     4321 cggcggattc tcccacaagg gcctgaccgt gacgccgttc tgctccgggc atattctggg
     4381 cggagtcggc ctggttctcg aaaaaaattc caaacgggtc ctggtcacct ccgatatttc
     4441 tgacatcccg aaggagacat cccgggccct ctttctcccg caggggaatt ttgatctcgt
     4501 gatcagtgaa gggacccggg gacttgactg cggagacacg gacctgccgc ttcgtgaaga
     4561 cgaggaagaa caagccatcc gcattctttc acgggttctc tcccgaaaag gaagcgttct
     4621 cttccctgct tttgtcatcg aacgtgccca atccgtcgcg cggctggtca tgcgtgccag
     4681 ggattcgggc cggataccgc ccggtgtgcc actctacatg gccggacttg ccgacagcat
     4741 gacagcactg catgcccagg cactcggcct gcgccttgac tggaaaccgg ttccctcacg
     4801 cgactggttt gacgatctcc tctcccggga acaggccatc cttgttgccg gctcgggcat
     4861 gctcaattct ggatccggtg cagcccggct tgcggagcag cttctctccg accgcaagcg
     4921 cgaacacgga atcatcttta cgggatacct caccccccag agtcccggcg gacgactgat
     4981 gcaccgggaa cgcagtccct tgagcgggca cacccatgcc tgcatcaatc agcgaatcgt
     5041 cgatgtccgc acaaacgaga tccaccaggt ccatctctcc tgccatgcct cgggggacag
     5101 tctcgtcgat ctggtccgcg gaatggaacc ggcaggcgta atcctggtcc atggtgaccc
     5161 tgatgctctc gccaccctgt cggctgccat gcggcttgcc ggactgaccg atatccgtgt
     5221 tcccgaaaac ggcgagagca tcagcttttc cgggaatccg gccgccaccc tgatcccgcc
     5281 gctggatcca tccgccgaac cgatgacggt tgtcgagcgg aatccctgga atggaagagc
     5341 cctgcgggcc ctcgccggag aaaacgccat cctcccggat ggggagtccg cccgcaatcg
     5401 ccttcagtcc atcacgaacc tgcttctgga gagagccatg cgtgacggaa acctcggtcg
     5461 ggcaaagagc atcctcgacg agtccggctc cttcatcccg ggcgatgatc gtgaagcgat
     5521 caccctcagg atagacgaga tcgccgcctg gtgtccccaa aacgatgacc gcacccctaa
     5581 ccagcggcga gactgagtct tgtttgatct ccggacatgg ctgtttccca aaaaaaagaa
     5641 cccgccggtg aaccgatcac cagcgagtcc cttgcaggtt ctgcacaccc ttacttgttg
     5701 gttgctgtag tctggttctt cttgttctct tcatcagcca gacgctgctg ctcgagctca
     5761 tggagacgct tcatctcggc ggcctttttg ggatcatact cgaacacgta tttggggaga
     5821 tcccatttcc aggtcagagc caaaagatgg cgtgtccctt caacaccgaa gggaagcgta
     5881 aaggcataat cgaaactgac aaggtgttca cccatgggga gcacagccga aatccccagc
     5941 gagagttcgc tcatgtcctc gtccccgagc tgcaatccgc cccggaaagc gaagatgttg
     6001 tcaaaccacc aggattcgaa cccgaaatgg tatgaagcct tggtcgcggt attgtccgat
     6061 gaatcatact tcccgtacgt gacataattg aaggtcaccg tcatgttctc gaaaaagatc
     6121 agctttggga aaaagatcga gccgccaagt ttgacgttgg tcggactgta gtcctctcct
     6181 ccgtcaaaga cgctgaaatt cggcttgaga agattttcga agacaactgc cagtctgatg
     6241 ttttccgaaa aattgtagat cactcccagg tcaattccga tcgtgaacac cgaatcgttg
     6301 ggattgtccg gcaaggtccc ccaatcagcc gtcttggtaa atgcgagaga gtacatgtcg
     6361 atgttgaacc cggccgcaag tttggctccc ttgaacagga catcatccag aaccttggca
     6421 tagttgagcg tgatcatggt ctccttgaca agctgggcgt tgtcccgcat gtcaaaggca
     6481 agtgccatgg caccgtcctt gaacatgaac tcctcgttcc cgagtgtcaa ccccatgaag
     6541 agccagcgaa tcagccagga aaggttcacg tcatttgtga acggcatgag gaaggtcccg
     6601 ttcatggcat cgaacgagga accatcattc agatcgccca tggtgagcgg ctttccccac
     6661 tggagattga gttccagact ggttgaacgg gccgtaccgg ccgggttgta ctggacgctg
     6721 ttgacgttcc cgaaaattcc gatcatggca ttggccatgc cacgggcccg ggcatcgcgc
     6781 ccgatatcct cgaaatacgc actccccggc acggccaccg ctgtcagcag caccgccagc
     6841 gctattccct tgcagagatt cttcatgttc tgtctccttc catcctatcg cagtatgaga
     6901 acactgccgg aatacacact tgagccggct atgatctggt agacgtacac gccgcccctg
     6961 actttgaatc cattcctgtc tttgccatcc cagaagactt ctccctgggc atagcttcca
     7021 tccctggtaa agtgttgcac cagctgtccc tgcatgttga agatcttgac ctcatagctg
     7081 ctgtaggcct tggcaaaggc gaagctgagt ttcatattgg agaactgccc gtcaccacgc
     7141 cccggagtga acggatttgg agaagccttg accgaatgga tcgcctgggt cgttgctgtc
     7201 gccgtggcca tcaccgcgta ataggtgtgg agatagctga cacgagccct gatcgtcttt
     7261 gtctgggtgt caacaacacc accgacaaag acccattcct tggtcgatgg cttccagtac
     7321 cagatcccga gccggtcatc cgtccatccc tgcgaagaga gatcaccgta gtgcaggacg
     7381 atctcgacat cctttttgaa accgttttcg agcgtcacgt tatccgcaag gttgacaatc
     7441 ttgaagtgcc agaccgagag cgtcgtactg tcggtcggtc tggtgattac acgctccgaa
     7501 gcattggcat acgattcctg gtcatcagtc atatcctcga tggcaatatt gacggagatg
     7561 ccttcaaaga gtgtcccggc cggaaccacg agtttggtga gggcatcttc gggacaaatg
     7621 acctgctggt ctcccgtcgc ctgcttgttg agtttgccgt acaccatgat caggttcgtc
     7681 aactgaagca ccgtattctg tacggcactc acaccggaga cctggagggt gtcgtaattc
     7741 accggaacga tgacccgacc gttattggtg acggtttctc cgatcggcgg catgacgacg
     7801 agccggtaag ccagcgacgg cggaatcttg tcaaccacat aggccccggt gttcgtgttg
     7861 acaagggcct ggaatgcctt tccttcaaca ttggttgcga tggattcact cgccgtatcc
     7921 ttgagggtca cggtcggccc aagcccgagg gggagaacca caccacggat ccggccaaac
     7981 cgggcattct cgaccacgag tttgtcccgc tggaggcccc tcgccagaat gtactgctcg
     8041 cttcctccat agcgtacccg agccgtcacg gtattagtca ggctcttcgt tgaagcctcg
     8101 atcatggcct tgaaggcgtt cttgtcgtaa ctcagggcca gcgtcagggt gtccgacatc
     8161 tgctgcccaa aattggttcc gacccagcgc aggatgaggg tgttggtggc atgcgtgtac
     8221 gccacggacg ccgaacctgt cgccagtgtg ctggtgatac cctcaacggt aaaggtattg
     8281 ctcaatggaa tgaccacaga gttcaccgag aaccggcggg acttgttctt gatcgcataa
     8341 cgaacagtgg ccgcatcatc catggtgtag atcacggtat tggtcacagt ctcccagata
     8401 tagggagcct tggccccgac gatataggct tcgggatcct cttccttgat gaagacgtcc
     8461 aagcgctgtt gtgcaccggc aatcgccgtc ggcgccgtca ctgcgacata cccgttcccg
     8521 tcatcattgt ccgccttgaa gatgtaatca aaggagagcg cattgttcac actgtgcctg
     8581 agggtaaacc tcaaggtatc cttctgcccg ggccggagga atccggcacc ttcatagctg
     8641 acaaagacgt tcgtggagtt ggtcacggtc accacaccgc cccgatcact catcagaccg
     8701 ctcatattgg acaatgcttg cggtacctga agaagaacgc gatagacatt gttcgaccct
     8761 ctgccccggt tggtgaagga gagcgtcatc acattggtga caaccgaacc cgggtcattg
     8821 gccgcataga cccaaaccgt attgagccaa ccggcagccg ccacggcagg catggtgaaa
     8881 gcctgcgcca tggcaccggt tgtactctcg gctgtcattg cgtatccgtt cccgttgtcc
     8941 acctggacct gccaagcgac gttggtcgcc ccatgactga gcccgtcttc gagaacaaag
     9001 gtcagggtat cactgcttcc attggcgatt gccgccggac cgtactcgac aaaatactca
     9061 ttcccgagat tggtgatggc cgcaccactg accctggtgc tggcaacgga aacgatcctg
     9121 ttgaacactt ccggaatctt gacacgagcc cgcagaaccg cggtgtctcc gctcccggtg
     9181 ttgttgatat gaacctcgac cgtatttgtc ccggtcgttg tatcgatggc attcggactg
     9241 atgcgggtct gtggcggtgt cacaacccgc aggatgttcg aaaccccttt gaccagagcc
     9301 gggtcaccaa gcgtactgtt actgacaatc gcattgaggg taaaactccc aacctcatgc
     9361 tgcgggttga ccaaagcaat ggagaaaaca tcgccctgcc cggggcgcac accctgaaga
     9421 tagcgtgcat cgaccaaacc gcttggcgca tgcgtgacat ttgttgaaac tgcagccaaa
     9481 acctgtgtca tcacattgct catcacaccg ggtacggtca gcacgaatcg ttccaagagg
     9541 ttgctcccgc tccctcggtt tgtcacccag acccgcagtg tggcgttggt atgtgtggta
     9601 tagatgtccc tgacaggaag gccgaactcg acaaggggtt ccggcatgac aaactcgatc
     9661 cggttggtcc cgtttgcaac ggccgcattg gtaaaatagg ccatcgtgtc gaatttgacg
     9721 ttgacaccaa gactggtgct ggtattcccc tgggcaaacc cgtcacgaat cgtgagcgcg
     9781 agctggtcac cgcccttggg tgcgatcggt gtcggataga agagggagaa gtaattggaa
     9841 aagagtgtga tggttgcacc acgtgcactg gtgattccga tcccgt
//
LOCUS       DAOSHE010000022         9723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5960_length_9723_cov_4.098055, whole genome shotgun sequence.
ACCESSION   DAOSHE010000022 DAOSHE010000000
VERSION     DAOSHE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_9723_cov_4.098055"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLD82_01305"
     CDS             <1..328
                     /locus_tag="PLD82_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG64054.1"
                     /translation="ESHTDFVLAAQAEEIGAFGITLLLLLVLALVWRIFRVASRSTDD
                     FSRLFCFGVAMMIGWQALVNAAMVTGLVPTTGLPFPFFSYGGSSLLMLSMALGIVLNI
                     SRSRTA"
     gene            343..1449
                     /gene="murG"
                     /locus_tag="PLD82_01310"
     CDS             343..1449
                     /gene="murG"
                     /locus_tag="PLD82_01310"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOG64055.1"
                     /translation="MVVFVTAGGTGGHIFPGLALYEELVRRGHEVLFVGSSADRVRFE
                     SIERLGNRFVGFRVQQLYRRKIWKNFSGLLLFIRAVRAARALIKSFSPDVCVGMGGFA
                     SAPLILACKLKGIPYCIAEQNAIPGRANRFLALWASAVFLNMRQAFDGFKGRIRERCH
                     ITGNPVRPGFGAVSQREARQRMGLVPKGFVLGVTGGSQGARAINSAMASFARKHRKIT
                     LLWSSGKAGYAEVTEACAGLENVFVHAFVEDMAAFLSASDLVVSRAGATSLSELASCS
                     VPSVLIPYPWAADDHQTRNARAWEAAEAAIVVPEGADFGERLENTINDLVKKPQRLEM
                     MRKAAGRLHDATSLGRMADLVEGSGRSRRGSEEG"
     gene            1446..2408
                     /locus_tag="PLD82_01315"
     CDS             1446..2408
                     /locus_tag="PLD82_01315"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG64056.1"
                     /translation="MRMERELKAERIGVLCGGMSGEREVSLRSGENVYQACRRLGLDC
                     VKIDVDTDIAASLRDEKITLAYIALHGKWGEDGCVQGLLELLRIPYTGSGVMASAIGM
                     NKLYTKRILEAEGIPVPGCVVIDQADPKGSAEEAIKRFGLPLVAKPVEEGSSIAVTIA
                     HDRETLLGAARDLAQRYHQSIIECFIAGKEITTGVLGTGSAARALPILGLEPVAGKEF
                     YDYEAKYTPGMTRFILPADLPPEITRRAQDLAVQVHRTIGCRGVSRVDAMVQPDGAIS
                     IIEINTLPGMTATSDLPAEAAEAGISFDGVVERILLSALEDCTF"
     gene            2558..3793
                     /gene="ftsA"
                     /locus_tag="PLD82_01320"
     CDS             2558..3793
                     /gene="ftsA"
                     /locus_tag="PLD82_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG64057.1"
                     /translation="MNSTIAVGLDIGTTKVSVVVGERDVNGLIEIKGVGTAPSNGLRK
                     GVVVNIDATVESIRKAVEEAELMAGLSIKEVYVGIAGGHIEGMNSRGVIAVSSKGREI
                     TQPEVERVIDAARAVVIPSDRDVIHVLPQEFIVDEQDGIKDPVGMCGVRLEAEIHIVT
                     GSVTSMQNIVKSVQRAGLDVRDIVLQPLASANAVLSEDEKELGCILIDIGGGTTDVLM
                     YVDGAVWHTGVLSIGGGHLSNDLSVGLRTPTVSAEVIKRKYGCAMRGLVNPAEMIEVP
                     AVGGRPPQRVPRTVLADILQPRMEEIFSLVNREVQMTGLREMIAAGVVITGGACLVEG
                     TADVAEEVFKFPVRVGMPRNVSGLKDMVASPIHATAVGLCQYGIIDQEIKGNNLQPRG
                     GRLFGDFRDRVKGWLKEFF"
     gene            3886..5055
                     /gene="ftsZ"
                     /locus_tag="PLD82_01325"
     CDS             3886..5055
                     /gene="ftsZ"
                     /locus_tag="PLD82_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG64058.1"
                     /translation="MTIEMVEPSSNPTVIKVIGVGGGGCNAVNRMISCRMENIQFIAA
                     NTDAQVLFKSNALEKIQLGQRLTKGLGAGASPDVGEKACEEAQERIAEVLQGADMVFV
                     TAGMGGGTGTGAAPIVAKIARSLGALTVAIVTKPFHFEGKKRMEQAEEGIRRLIEHVD
                     TLITIPNQNLIKISDRKTSLEDGFRMADDVLRQAVQGISDLITKTGHINVDFADVRKI
                     MSNKGQALMGVGIAGGENKGAAAARAAVNNPLLEDSSIEGAESLLVNITSDHNFSITE
                     YQEVVDAVREKVSQDAEIICGNVFDDRYKDEVMVTVIATGFRHARERVVAGGNVEFVS
                     NNDFLGGVRTASQYDKVPPSREAVPEPVFAGGAYSGYSDDNIDIPAFLRNRNQHR"
     gene            5217..6113
                     /locus_tag="PLD82_01330"
     CDS             5217..6113
                     /locus_tag="PLD82_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG64059.1"
                     /translation="MRSTVNSWRVGVWCLVALLVGCGETKIDEHKNIKDGKNVVVPSR
                     KEYPNMVFIPAGEFLMSTSSTDQGRRRVHLKAFYIDIYEVSNAQYEGFISDGGYAREE
                     LWSKEGWAWRSSNGVTMPKWWKTGRYRIGPMHPDYPVGGVSWYEAEAFARWAGKRLPT
                     EAEWEMASRGMDGRLFPWGNDPVDADGVYRANFEPVNDGNMHAGRIDSYPLGKSPWGC
                     FNMLGNVWEWVADSATKNGRWYRTLPDKNPVFTAETGYKLMRGGSWYKGFSVYDYFFR
                     LAIEPGVMAYDDFGFRCARDAD"
     gene            6138..6404
                     /locus_tag="PLD82_01335"
     CDS             6138..6404
                     /locus_tag="PLD82_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64060.1"
                     /translation="MMRLLMLLLVFGLLVVTGLIVHENAAETARMTVFGRVVFEGVPV
                     PVLILVSLVAGMLLMMPFLAVARIRKRPTKKDSVEPSSDRNSPG"
     gene            6401..7435
                     /locus_tag="PLD82_01340"
     CDS             6401..7435
                     /locus_tag="PLD82_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG64061.1"
                     /translation="MRIRLLPVLLLFILASIPVFPAVRTIDDARKAIASGDEPGARAI
                     LQTILRSGGDSPGRAGVLALLISLEHLVGPRIALLEEYVRRYPREKDWRGMVKELAWL
                     NLASGKYADAALLLTRLVAVPEERESALLGLVRIAIAQENWDQAERSCTALEREYPDG
                     VFRPTVLACQARIHLARGRNKEASLAWHAIIEKFGRSDEAPGAWLALGEMAATAKDIR
                     LAQDYLTKLVRAFPASMEAGLARSRLRLLAVENPRTSNLRVWEVQLAVYHAKSQAEAF
                     VAKLRAAGYLSFVQQETVGGRIQYSVRMGYFRTREDAAQVLAGMQHKGYEGFIRTRTV
                     TVTEDVLGRE"
     gene            7422..>9723
                     /gene="mutS"
                     /locus_tag="PLD82_01345"
     CDS             7422..>9723
                     /gene="mutS"
                     /locus_tag="PLD82_01345"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG64062.1"
                     /translation="MGESNQTPMMQQYLAIKKRYGGMLLFFRLGDFYEMFYQDAEEAS
                     ALLGLTLTSRNGMPMCGVPWHAAEGYIARILKAGKRVAICEQVEDPAKTKGLVRREVT
                     RVITPGTVTDEKLLQTDENNFLMAIWLDQHNAALAVADITTGEFGVASCQGDVQKLNA
                     FLEDEIIRFAPSEIVTLASQATKIPVGLIQGISLTTVADHLVHPQTARETLCAQFQTA
                     SLDGFMINEEDGSLQAAAIALASLREIRLGAVESITTIKRMDSGGTVMLPAITQTNLE
                     LVRSLSGGEERTLVSVLDRTATPMGKRRIRQNILAPMLDPVAINKRLDLVSVQTADRV
                     FLDAVIQSLGRIRDLERLASRVALGRANARDLVAIAASLEEVRVLRKRLLEKPEYFEI
                     CQGIDPFDRLIAALREALLDEPAAGIDEGGMIRSGFSPELDELRGLQSGGEEALAAMQ
                     QRERERTGIGSLKIRYNRVIGYFIEVSKSNLASVPSEYIRKQSMVGGERFVTDELREY
                     EERILGAQERIIELERSLFKTLTQETANWVARINAAADRIAEVDLLACFAKNALDYGY
                     MRPVVDGSDRLSIVEGRHPVIERVIPEPFVPNATELDLAENRIKIVTGPNMAGKSTYL
                     RQTALIVLMAQIGSWVPAASAHIGVVDRIFTRVGASDNLARGQSTFLVEMLETAAILN
                     NATARSLIIMDEIGRGTSTYDGLSLARAVAEYLLEHVEVGGRTLFATHYHEMTVLGER
                     EGAGNLNVQVREWNDEVIFLRKVVPGA"
BASE COUNT         1993 a         2528 c         3106 g         2096 t
ORIGIN      
        1 cgaatcacat accgactttg tcctggcagc ccaggccgag gagatcggcg ctttcggaat
       61 cacgctgctc ctgctgctgg ttctggccct ggtctggagg atcttcaggg tcgcttccag
      121 atctacggac gacttttcca gactcttctg tttcggtgtg gccatgatga tcggatggca
      181 ggcattggtg aatgccgcca tggtgacggg tctggttccg acgaccgggt tgccgtttcc
      241 gtttttcagt tatggaggtt cgtcgctcct catgctcagc atggcattgg ggatcgtgct
      301 caatatttcg aggtccagga cagcgtgaag gagaacaagg ccatggttgt gtttgtcacg
      361 gcgggtggca cgggagggca tatttttccc ggactggcac tgtatgaaga actggtgcga
      421 cgggggcacg aggtgctctt tgtcggatcc agtgcggacc gtgtccgttt tgaatccatc
      481 gaaagactcg ggaaccgctt tgtcggtttt cgggttcagc agttgtaccg caggaagatc
      541 tggaaaaatt tttccggcct gcttctcttc atcagggctg tccgcgcagc gcgcgccctg
      601 ataaagtcct tttcgcctga tgtctgcgtc gggatgggcg ggtttgcgag tgcgcccctc
      661 atactcgcct gcaaactcaa gggcatcccg tattgcattg ccgaacaaaa tgccatcccc
      721 gggcgggcca accgttttct ggctctctgg gcgagtgccg tttttttgaa catgcggcag
      781 gcctttgacg gattcaaggg aagaatccgt gaacgctgcc atatcaccgg aaacccggtc
      841 cgtcccggtt ttggcgcggt ttcacaacgc gaagcccgcc agaggatggg actcgttccc
      901 aagggatttg tcctgggcgt aacggggggg agccaggggg cgcgtgcgat caatagcgcc
      961 atggccagct ttgcgcgcaa gcatcgcaag atcacgcttc tctggagttc cggcaaggcc
     1021 gggtatgccg aggtgacgga agcctgtgcc gggctggaaa acgtctttgt ccacgccttt
     1081 gtcgaggata tggccgcctt cctgtcggct tctgatctgg ttgtctccag ggccggggcg
     1141 acgtcccttt cggagcttgc gtcctgttcg gtgccgtccg tcctgatccc gtacccttgg
     1201 gccgcggatg atcaccagac caggaacgcg cgggcctggg aggccgccga ggcagcgatc
     1261 gtcgttccgg agggcgctga ctttggcgag cgtctggaaa acacaatcaa cgatttggtc
     1321 aaaaagccgc aaagactcga aatgatgcgc aaggctgcag gcagactcca tgatgcgaca
     1381 tcccttggca ggatggcgga tctggttgag ggatccggca ggtcgagacg cggttcggag
     1441 gaaggatgag aatggagcgt gaattgaagg ctgagaggat cggcgttctc tgcggtggca
     1501 tgtctggcga gcgcgaggtt tcactgcgga gtggcgaaaa cgtgtatcaa gcctgtcgca
     1561 gacttgggtt ggattgtgtc aagattgatg ttgatacaga tattgctgca agtctgcgcg
     1621 acgagaaaat caccttggcc tatatagccc tgcatggaaa atggggggag gacggctgtg
     1681 tccagggcct cctcgaactt ttgcggatcc cctataccgg ctccggcgtc atggcctccg
     1741 ccatcgggat gaacaagctc tatacaaaaa ggattctcga ggccgaaggg atccccgtcc
     1801 cgggctgcgt tgtgatcgac caagccgatc cgaaagggag tgcggaggag gccatcaaac
     1861 gcttcgggct tcctcttgtg gccaaaccgg tcgaggaggg ttcgtcgatt gcggtcacga
     1921 tcgctcatga ccgggaaaca ctcctgggtg ctgcccgcga tctggcacaa cgctatcatc
     1981 aatcgatcat cgagtgcttc attgccggga aggagatcac gacgggcgtg ctcggtacgg
     2041 gcagtgctgc ccgggccctt cccatcctcg gattggagcc ggtggccggc aaggagttct
     2101 atgactacga ggccaaatat actccgggaa tgacacggtt tatccttccg gccgatcttc
     2161 cacccgagat cacccgtcgt gcgcaggacc tggcggtcca ggtccatcgt acaatcgggt
     2221 gtcggggggt ttcacgcgtc gatgccatgg tccaaccgga tggtgcgatc agcattatcg
     2281 agatcaacac cctcccggga atgacagcga ccagtgatct cccggctgaa gcagccgagg
     2341 cgggcatcag ttttgacggg gttgtcgaga gaatcctgct ttcggcgctc gaggattgta
     2401 ccttttgatc ctcggtgtgg tgggagtgag tcccgaaggc tgtggttggg cctgacggaa
     2461 aaaagatttt ttaaggtttt cataaaaaaa gacttgcgct ggctggaatg aatccgtaca
     2521 atggctaaat tccggcaatg gttttcggga ggggcatttg aacagtacca ttgcagtcgg
     2581 tcttgatatc gggacaacca aggtttcggt tgtcgtcgga gagcgggatg tcaacggact
     2641 gatcgagatc aagggtgtcg ggactgcacc atcaaacgga ttgcgaaagg gcgttgtcgt
     2701 taatatcgac gccacagtcg agtcgatccg caaggcagtt gaagaagcgg aattgatggc
     2761 gggcctgagc atcaaggaag tttacgtcgg aattgccggc ggtcacatcg aagggatgaa
     2821 ttcccgcggg gtgattgccg tctcgagcaa ggggcgcgaa atcacccagc ccgaagtcga
     2881 gcgtgtgatt gatgccgccc gggcggttgt cattccgagt gaccgggatg tgattcacgt
     2941 cctgccccag gagttcattg tcgatgagca ggacgggatc aaggatccgg tcgggatgtg
     3001 cggtgtccgg cttgaggccg agatccatat cgtgaccggt tccgtgacct cgatgcaaaa
     3061 catcgtcaag agtgtgcagc gtgccgggct cgatgtccgg gatatcgtgc tgcaacccct
     3121 ggcttcagcc aatgcggtcc tctccgagga cgaaaaggag ctgggctgca tcctgatcga
     3181 tatcggaggc gggacgacgg atgtcctgat gtatgtggat ggcgccgtct ggcataccgg
     3241 ggttctgtcg atcggtggtg gacacctcag caatgacctc tcggtcggat tgcggacgcc
     3301 gacggtctct gccgaggtga tcaaacggaa gtacggttgt gcgatgcggg gacttgtcaa
     3361 tcccgcggag atgatcgagg ttccggcagt tggtggccgg ccaccccagc gtgttccgag
     3421 gacggttctg gcggacatcc tgcaaccccg catggaggag attttctccc tggtcaaccg
     3481 agaagtccag atgacgggac tcagggagat gattgcggcc ggggttgtga tcacgggagg
     3541 cgcctgtctg gtcgagggga ccgccgatgt tgccgaggag gtcttcaaat tccccgtcag
     3601 agtcggaatg ccaaggaatg tgagcgggct caaagacatg gtcgccagtc cgatccacgc
     3661 gacggctgtc ggtctctgtc agtacgggat catcgatcag gagatcaagg ggaacaacct
     3721 ccagccgagg ggcggacgat tgttcggaga tttccgggat cgcgtgaaag gttggctcaa
     3781 ggaattcttt taaagtatat tgatagagcg ctttcccggc tggatgggac cccggggaag
     3841 ggtttgtata tgatccaggc agtacgtcat tcatgggagg ggaagatgac gatcgagatg
     3901 gtggaaccga gcagcaatcc tacggtgatc aaggtgatcg gagtaggggg tggtggctgc
     3961 aatgcggtca accgcatgat ttcctgcagg atggagaata tccagttcat tgcagccaat
     4021 accgacgcac aggtgctttt caagtccaac gcgcttgaaa agatccagct cgggcagcgc
     4081 ctgacaaagg ggctgggtgc cggagcgagt ccggatgtcg gagaaaaggc ctgcgaggaa
     4141 gcccaggagc ggattgccga ggttctccag ggtgccgaca tggtgtttgt gaccgccggg
     4201 atgggcggtg gtaccgggac gggggcagcg ccaattgttg ccaagatcgc ccggagtctg
     4261 ggtgccttga cggtcgccat cgtgaccaag ccgttccatt tcgagggcaa aaagcgcatg
     4321 gaacaggccg aggagggcat ccgcaggctc atcgagcatg tggacaccct gatcacgatc
     4381 cccaaccaga acctgatcaa gatttccgat cgcaagacct ctctcgaaga cggattccgg
     4441 atggcagatg atgttcttcg ccaggcagtc cagggcatct ccgatctgat caccaaaacc
     4501 gggcatatca atgtcgattt tgccgatgtt cgcaagatca tgtccaacaa ggggcaggcc
     4561 ctgatgggtg tcggaattgc cggaggggag aacaagggcg cggccgctgc ccgggctgcg
     4621 gtcaacaatc ccctcctcga ggattcatcc atcgagggag ccgaatccct cctcgtcaac
     4681 atcacctccg accataattt ctcgataaca gaatatcagg aagttgtgga tgctgtccgc
     4741 gaaaaggttt cccaggacgc cgagatcatc tgcgggaacg tctttgatga ccgttacaag
     4801 gacgaggtga tggttacggt gattgcgacc ggattccgcc acgcacgcga gcgggttgtt
     4861 gccggtggta atgtcgaatt cgtttccaac aatgattttc tgggcggggt ccggactgcg
     4921 tcccagtatg acaaagtccc gccatcccgc gaagctgttc ccgagccggt ttttgcgggc
     4981 ggagcctatt ccgggtacag cgatgacaat atcgatattc cggctttcct gcggaacaga
     5041 aatcagcacc gctgaacaat tcaggggata tcctgagagg gacaccggcg tggtgtctct
     5101 cttttttttg ggaacaatct tcagccatgg tcgggctgct ccatccccgg tttgaaccgc
     5161 aggccggaaa ctggtatact agagagaagc gggaaatcat ccggcggagg aagaccatgc
     5221 ggagtacagt caattcgtgg cgggttggtg tatggtgtct tgtcgctctg cttgtcgggt
     5281 gcggtgaaac caagatcgac gagcacaaga atatcaagga cggaaagaac gtggtagtcc
     5341 cgtcgagaaa agaatatccg aacatggtat ttattcctgc cggggaattc ctgatgagta
     5401 catcctcaac ggatcagggg agacggcgcg tccatctcaa ggctttttac atcgatatct
     5461 atgaagtctc gaacgcacag tatgaaggat tcatcagtga tggcggatat gcccgcgagg
     5521 aactctggag caaggaagga tgggcttggc gttcgtcaaa cggggtcacc atgccgaagt
     5581 ggtggaagac tggccgctac agaatcggtc cgatgcatcc tgactatcca gttggtggtg
     5641 tctcatggta cgaagctgag gcctttgccc gttgggcagg gaaacgtctt ccgacggaag
     5701 cggagtggga gatggcgtcc cgggggatgg atgggcggct cttcccctgg gggaatgatc
     5761 ccgtcgatgc cgatggtgtc tatcgggcca atttcgaacc ggtcaatgac gggaatatgc
     5821 atgccggccg gattgactcg tatccgctgg ggaaaagtcc ctgggggtgt ttcaatatgc
     5881 tcggaaatgt ctgggagtgg gttgcagaca gtgcgaccaa aaatggcaga tggtatcgta
     5941 cccttccgga taaaaatcct gtctttacag cagaaacagg ctacaaactc atgcgcggtg
     6001 gttcatggta taaaggattc agtgtgtatg attatttctt ccgtcttgca atcgagccgg
     6061 gagtcatggc gtatgacgat ttcggttttc gttgtgcccg tgatgcggac tgatccggaa
     6121 tccacggaaa tggaggcatg atgcgcctgt tgatgctgct tttggtgttt ggcctgctgg
     6181 ttgtgacggg tttgatcgtg cacgagaatg cggccgagac ggcccggatg acggttttcg
     6241 ggcgggtcgt ttttgagggg gttcccgtgc cggtcctgat tcttgtttct ctggttgccg
     6301 gcatgctcct catgatgccc ttcctggcag tcgcccgtat acggaagcgt ccaaccaaaa
     6361 aagattctgt tgaaccttca tcggacagga acagtccggg atgagaatcc gtcttctgcc
     6421 cgttctgctt ctgttcatcc ttgcttcgat tccggttttt ccggctgtgc ggaccatcga
     6481 tgatgcccgc aaggccatcg cctccggtga cgagccgggg gctcgtgcca tcctccagac
     6541 gattctcaga tccggcgggg attcgcccgg cagggccggg gtccttgcgc ttctgatttc
     6601 cctcgagcac ctcgtgggcc cgaggatcgc actcctcgaa gaatatgtcc ggcgctatcc
     6661 acgcgagaag gactggaggg gaatggtcaa ggagctggcc tggctcaatc ttgccagcgg
     6721 aaagtatgcc gatgccgcac tcctgttgac gcgactggtc gctgttcccg aggaacggga
     6781 atccgccctc ctggggctgg tgaggattgc catcgcccag gaaaactggg atcaggccga
     6841 acgttcctgt actgcccttg agcgggagta tccggacgga gtgttccgcc cgacggtgct
     6901 ggcatgccag gccaggatcc atctggcgag gggacgcaac aaagaagcat cactggcctg
     6961 gcatgccatc atcgaaaaat tcggtcgatc cgatgaagcc ccgggggcat ggctcgctct
     7021 tggcgaaatg gccgccacgg ccaaggatat ccgcctggcc caggactatc tgaccaagct
     7081 ggttcgggct tttccggctt caatggaagc ggggctggcc aggagccggc tgcgtctcct
     7141 ggccgtggag aatccccgga cgagcaatct gcgtgtctgg gaggtgcaac tggcggtgta
     7201 tcatgccaag tcccaggctg aagcctttgt ggcgaaactg cgtgccgctg ggtatctttc
     7261 ttttgtgcaa caggagaccg tgggcggaag aatccagtac agcgtccgga tggggtattt
     7321 cagaaccaga gaggatgcgg cacaggtcct tgccgggatg caacacaagg gctatgaggg
     7381 attcatccgc acccggacgg tcacagtcac ggaggacgtc cttgggcgag agtaatcaga
     7441 cacccatgat gcaacagtat cttgccatca aaaaacgcta tggcgggatg cttcttttct
     7501 ttcggttggg cgatttttac gagatgttct accaggatgc ggaggaagcg agcgctctcc
     7561 tcggcctgac actgacctcg cggaatggca tgccgatgtg tggcgtcccc tggcatgccg
     7621 ccgaagggta catcgcccgg atcctcaaag ccgggaagcg tgtcgcgatc tgcgaacagg
     7681 tggaagaccc ggccaagacc aagggtctgg tccgccgtga ggtgacccgg gtgatcacac
     7741 ccgggacggt tacggatgag aagcttctcc agaccgacga aaacaatttt ttgatggcca
     7801 tctggctgga tcagcataat gccgctctgg cggttgccga tattactacg ggtgaattcg
     7861 gcgttgccag ttgccagggc gatgtccaga agctgaatgc gtttctcgag gacgagatca
     7921 tccgtttcgc tccgagcgag atcgtgacgt tggcctcgca ggccacaaag attcccgtcg
     7981 gcctgatcca ggggatctct ctgacgaccg tggccgatca tcttgtccac ccccagactg
     8041 cccgggagac gctctgtgcc cagttccaga cggcttccct ggatggtttc atgatcaatg
     8101 aggaggacgg ttccctccag gcggcagcca ttgccctggc cagtctcagg gagatccggc
     8161 tgggagcggt cgaaagcatc acaacgatca agaggatgga ttcgggcggg acggtcatgc
     8221 ttccggccat cacccagacc aacctcgaat tggtccgctc cctctccggt ggtgaagaac
     8281 ggacattggt ctctgttctt gacaggacgg cgactcccat gggaaagaga cggatcaggc
     8341 agaacatcct tgcacccatg ctggacccgg ttgccatcaa caagcgcctg gacctggttt
     8401 cagtccagac agcggacagg gtctttctcg atgccgtcat ccagtcactc ggcaggatcc
     8461 gggatctgga gcggttggca tcccgggtcg ctcttgggcg cgccaatgcc cgcgatcttg
     8521 tcgcgattgc cgcatctctc gaggaggtca gggttcttcg caaacgactt ttggagaaac
     8581 cggagtactt cgagatctgt caggggatcg atccgtttga tcgtctgatt gcggccctcc
     8641 gtgaagcact ccttgatgaa ccggctgccg ggattgatga aggggggatg atccggtccg
     8701 gcttctctcc cgaacttgat gaactgaggg gcttgcagag tgggggagaa gaggctctgg
     8761 ccgcgatgca gcagcgggaa cgtgagcgga ccgggatagg atcgctcaag atccgctata
     8821 atcgtgtgat cgggtatttc atcgaggtct cgaagtcgaa tctggccagc gtgccgtccg
     8881 aatacatccg caaacagagc atggtcggtg gcgaacgctt tgtgacggat gaactccgcg
     8941 agtatgagga aaggattctc ggggcccagg aacggatcat cgagctggaa cgctccctct
     9001 tcaaaaccct cacgcaggag acagcgaact gggttgcgcg gatcaatgcg gccgccgacc
     9061 ggatcgccga ggtggatctt ttggcctgct ttgcaaaaaa cgccctggat tacggataca
     9121 tgcgcccggt tgtggacggg agtgacaggc tgagcattgt cgagggcagg catcccgtga
     9181 tcgaacgtgt cattccggag cccttcgttc cgaatgcgac agaactggat ctggccgaga
     9241 atcgcatcaa gatcgtgacc ggacccaata tggccggaaa atccacttac ctgagacaga
     9301 cggcgctcat cgtcctgatg gcccagatcg gatcgtgggt gccggcagcc agtgcccaca
     9361 tcggtgtcgt ggacagaatc ttcacgcgtg tcggggcgag tgacaatctc gccaggggac
     9421 agagcacctt tcttgtggag atgctcgaaa cggcggccat cctcaacaat gcaacggccc
     9481 gctccctgat catcatggac gagatcggga ggggtacaag tacctatgac ggactctctc
     9541 tggcacgggc ggtcgccgag tatcttctgg agcatgtcga agttggcggc cggaccttgt
     9601 ttgcgacaca ttatcatgaa atgacggttc tcggggagcg ggagggcgcc ggaaacctga
     9661 atgtccaggt ccgggagtgg aatgacgagg tgatcttcct ccgcaaggtg gttcctggtg
     9721 cgg
//
LOCUS       DAOSHE010000023         9679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_5994_length_9679_cov_4.004780, whole genome shotgun sequence.
ACCESSION   DAOSHE010000023 DAOSHE010000000
VERSION     DAOSHE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_9679_cov_4.004780"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2357)
                     /locus_tag="PLD82_01350"
     CDS             complement(<1..2357)
                     /locus_tag="PLD82_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HOG64063.1"
                     /translation="MNLDTALADIFVPTLVIGLGGCGIEAVSQYRDIQRSETGREDWP
                     VHGFLGIDLETGHAPLEGFEWISLDCTCDDARQVPEWLQRDLVLRMRTNETLMQRRVG
                     RWAAWHAMPGIMQTIRNICERLTGESASRRMKSELEGRFGNIDHPRWPYRIKENDLKI
                     ILVASSSGGTGSALVRDLAVAVGSILPDAGVLLVLGVPGEDTAGTMQQQIYNTEATLR
                     EIAWWQTESGKLEFPGMEGGIRSKVPLFDAVHIIQCNAGQPTNPEALAIAIAGLVHMD
                     QARLFDDAQVALYDAETAEHRVFCEWHLSGAGFPLLPLADAAARLAVTRTFQDWGAGF
                     LKDSEGREIAKDAIEFANRLYQTILKQVPAADHSLITSECTIALDQGASQFCQADPAT
                     LLIQMRKCPVEKPVQLLLETVLPEHIQSQAQPLVEEALRLVAGQMVELLMQPLLESTD
                     ACKQNHARYNIPAVRKWMELLQEAVDRRMETIRSFEPDLLKIDTVTPFSAGGFSLLTQ
                     KKKRNQQAMENIWEQYRTMVVRYLASLSDQVLAEVLKKTAAWIVQDGFRILDGVQAWI
                     QKQSLEADVDLPEESVYRHVHVTGSFERDLRLVASKIRDGFETASGASLCRWDVFGHG
                     MHDRLVSYMRGRCESGSEGASLVQALQESAKKFVLRYAFRGLVDGKELTGKTYDSAVP
                     ETPPGLGDSRNLVRIHVGSHSAEDTVFLLPWWRWFSGWILACPYRTVSQITRFHQSWL
                     PSHTMNERLACSGIDPESGRYAMTESTPADDFSIEDETVEFIAAPS"
     gene            complement(2880..3581)
                     /locus_tag="PLD82_01355"
     CDS             complement(2880..3581)
                     /locus_tag="PLD82_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG64064.1"
                     /translation="MMRGRRRESGNFWPSFVDVAISLFFILMVVIIIMIVKREKEIDY
                     VKKFHMCVETSVSNINKTRIKSDENKSSGIKTSRDGFDIMMTFGADILFDKDKKVLKP
                     EGRRILETVINEILLPSYKNRVKEVMPDIQVVGHTDSGTSVNEKIYGNWELSTDRAIS
                     VVKLMIEKGLHPQEDCKTLLSAKGYSQYMSVTNDRRPGRGIIDELAALNRRIEIILHF
                     EQHKIKKHYESATNG"
     gene            complement(3578..5158)
                     /locus_tag="PLD82_01360"
     CDS             complement(3578..5158)
                     /locus_tag="PLD82_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG64065.1"
                     /translation="MIVRISMLQASSVAESSGILENNIDKIKAKSNTVRDVSNKALDG
                     IDSSEVRELVEELCVIHENGGKIEGMDVRTMIDDKFGSDADKAWHYLGLFVLLGLLGT
                     VIGLNGSVFQMSGILESDTAAVGAFSPVFSQMKGAFGTTLAGIAVTIFFGKLLHAVRG
                     RQHKALERLEFVLRSRLLPRYRPADDKSLLLEIKEGFLSAIEGFDGLTREYRAQAQNQ
                     REVQLRFSQSAATFRRTMDDFKTRTDSTADAFNDFTRVVTFMNNTVTEMARSTGDFQT
                     LVRDQFSSQTTDMAKMVESNTALVEQMKASRENEQQLHAAVSTLSSRIEKVFDEMAVT
                     IGERLGGLSDSIVAMQDSFKESLNENMKGLAGELTAQLGTVATRVDESLSGVAGSVRD
                     GFEVVAKNVSEQFAGLGDKFDGIEGTLKDSTTSVIQEFQGRLKEITDGMVSEIQTVSK
                     AVHDGQEVFIGAVGSFEDKMTGWADVAKLESDKVKSFVEVFSKWHKDVAQALEDFKIV
                     LGTRDWVDHIENVKKAHR"
     gene            complement(5332..7560)
                     /locus_tag="PLD82_01365"
     CDS             complement(5332..7560)
                     /locus_tag="PLD82_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64066.1"
                     /translation="MKKNPDKSLHDQKTETDAPVPDTAADTSSETISTQDHDTMGANE
                     SVDNRNPVVEEETPGETSAISGQEDGHTGTERILPDESGHVDENGSALVHDGEPDSGL
                     TVTRDTNGAVVVPVLPETGEQPHATETVTDAQTINQSRASERIPLAEMLDLRVFDTGE
                     DLSKFSRYIQPLLILPDTFQREVSSKTGMSFLLEVFAVGQKDCVVNQVAVSSRKWKKK
                     SDPPVICNTGMPCTIKSGKRVSLVFECRPESGLLSQQDIEISFKYNGSLSDSVEGRLV
                     WNVSERLFSWELSPSTIFLPEEQSFQHELMLRENEIEATPVLYESRNGGLVVSDSAAI
                     IPRKVLENTETGRSLVRYAISERIEAMGWSRVLVTDCEDEQGQYRSLSVNSADPSGVA
                     TEAWIMPGLDGPRLMAPVCNPGRRTVATGDLNLYAVADSSVTLTLRCDGRFMKLHTDS
                     CSIAPGKVARIRFSVLESAMKRDTAKDLAIRVSDGINETSIPVPLQRGASTIRMLEGV
                     RILAPSHIAIGEKVKILVMVPTTGSGELDIEIGYDLFRHRWPSPVVGKGGGGIEWHTL
                     ECEMDTGLMHPVDVVRCIVTAVSKTGNFFEQTVQLDAELVELDFAIVEPRGYGRSHFL
                     RFVEHPDSPGLLSSGEFVMALDREDNLPHGDVVWELLDGVADTPAGSIEEIREQTGVS
                     ISRARDDRNTWSLKIQKSLTGNTVRTAIIRAVSKPTGKGPELVASFTLLVPDTIQAKD
                     QA"
     gene            complement(7569..8342)
                     /gene="grpE"
                     /locus_tag="PLD82_01370"
     CDS             complement(7569..8342)
                     /gene="grpE"
                     /locus_tag="PLD82_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG64067.1"
                     /translation="MKSAFLGCFLVLVLSGIVSGLDGSPVKKTNIGVTDQIKSVPART
                     VPPVQNQEVSMITWLVMGGLVSILMVLIFMDVKVKNSMQEILNLLRYLSRQGDDASDG
                     RNSTFGKSLQEMASLGQVVSALGREMGSQRESMQQELGELRTMMKANAGLLEQMFRTK
                     NTVTKNEAQSLDDLSAQKKSADAGASTDIQTYLARHEIELINPGVGEIYNALRQEAIE
                     YVDEPGVGENRIVRCKKPGLRYRGRVVAKAEVIVARHES"
     gene            complement(8352..9353)
                     /locus_tag="PLD82_01375"
     CDS             complement(8352..9353)
                     /locus_tag="PLD82_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64068.1"
                     /translation="MNENAVLVRRLDVACLSLLPRTLPNTALSEDLSELSRFVRESAS
                     LYPRDPGAWAQRMELSKQWLEEFLLQIAPEGAGSLICIVPSYWSSAARSLLRQAVANT
                     GRFGDCSIMTDIVAGAAVLEYHGIREDALCVSEHATGVMHIRYQREDVAWRLASVDMT
                     RKFKSAGQGLKIVGKPGPNIEGNDLFGMDPLDVKHIADALSAQATNPVKFRYQTELVL
                     GLPGIGEIVWPASGPYPAFHAERVAVVSAPSEFYFPVSARYCPDQKYPAWICSPGLNK
                     DQKVRMIEKNMNVHFDIGLQLHDGMTARLFTEMIEPGNKKDSGTHSVMNIEIPHPVD"
     gene            complement(9350..9562)
                     /locus_tag="PLD82_01380"
     CDS             complement(9350..9562)
                     /locus_tag="PLD82_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64069.1"
                     /translation="MLSESKDFGFFSADLSTVPDAAFSDDGDWLVTRHHLWIEYQDEV
                     EVPRLVIELTNTGEMRELPLQRIQQS"
BASE COUNT         2374 a         2715 c         2158 g         2432 t
ORIGIN      
        1 gaaggagctg caataaattc tactgtttca tcttcaatac tgaaatcatc agcgggagtg
       61 ctttcggtca ttgcataccg cccgctttcg ggatcaatgc ctgagcacgc gagcctttca
      121 ttcattgtgt gagaaggcaa ccatgactga tggaaccgtg ttatctggct gacagtccgg
      181 tacgggcacg caagaatcca gcccgaaaac cagcgccacc acggcagcaa aaagacagtg
      241 tcttctgcag aatggctccc gacatgaatc cgtacaaggt tccgtgaatc acccaatccg
      301 ggaggagttt cgggtaccgc ggagtcatac gtttttccgg tcaattcctt gccatccacc
      361 aaaccccgga aagcatagcg caaaacaaat tttttggctg attcctgcag ggcttggaca
      421 agcgaagcac cctccgaacc ggactcgcag cggccacgca tatatgagac caaccgatca
      481 tgcatcccgt gaccaaacac atcccatcgg cagagtgagg cccctgaggc ggtctcaaat
      541 ccgtccctga tttttgaagc cacaagccgc agatcccgct caaaggagcc agtcacatgc
      601 acatgtcgat agacagattc ttctggaaga tcgacatccg cttcaagaga ttgtttctgt
      661 atccacgcct gtactccatc gagaatgcgg aatccatcct ggacaatcca tgcagcggtt
      721 tttttcagca cttccgcaag aacttgatct gaaagcgatg ccaggtatcg gactaccatg
      781 gttcgatact gctcccagat attttccata gcctgctgat tccgtttctt cttttgggta
      841 agcaaggaaa acccgcctgc agaaaacggt gttacagtat caatcttgag aagatccggt
      901 tcaaagctcc ggattgtttc catcctgcgg tcaaccgctt cctgcagcaa ttccatccat
      961 ttcctgacag cagggatatt gtatctggca tgattctgct tgcaggcatc cgtcgattca
     1021 aggagaggtt gcatcagaag ttcgaccatc tgacctgcaa ccagccggag agcttcttcg
     1081 accaggggct gcgcctggga ttggatatgc tccggcagga ctgtttccaa aagcagctgc
     1141 accggcttct caacaggaca ttttcgcatc tggatcagca aggttgcggg atcagcttga
     1201 cagaattggg aagccccttg atcaagagca atcgtacact cacttgtaat cagggaatgg
     1261 tctgcggcag gcacctgctt caagatcgtt tgatacaacc gattggcaaa ctcgatggcg
     1321 tcttttgcga tttcccgccc ttcggagtcc ttgagaaagc ctgcgcccca atcctggaac
     1381 gttctcgtca cggctagcct cgcggcagca tctgccaatg gaagcaaggg aaagccggct
     1441 ccggacaagt gccactcaca aaaaacacgg tgctcggccg tttccgcatc ataaagagcg
     1501 acttgtgcat cgtcaaagag tcttgcctga tccatgtgca ccagtccggc aatggcgatt
     1561 gcgagcgctt caggattggt cggttgaccg gcattgcact ggatgatgtg cactgcatcg
     1621 aaaagcggga ccttggaacg tatgcccccc tccattccgg gaaattcaag cttgccggat
     1681 tcggtctgcc accaggctat ctcccgcaag gtcgcttcag tattgtagat ctgctgttgc
     1741 atggtgccgg cagtgtcttc gcccggcaca cccaaaacaa gcaacacccc ggcatctgga
     1801 aggattgacc cgaccgcaac agccaaatca cggacaaggg cactccccgt tccccccgag
     1861 gaagatgcca cgagaatgat cttcaagtca ttctctttga tgcgatacgg ccagcgcgga
     1921 tggtcgatat tgccgaaacg tccttcgagc tcactcttca tgcgtcttga tgcactctca
     1981 ccggtcaggc gttcacagat attcctgatg gtctgcataa tcccgggcat ggcatgccac
     2041 gctgcccagc gaccgactct gcgctgcatc aatgtttcgt tggtccgcat gcgcaagacc
     2101 agatcccgtt gcaaccattc aggaacctgc cgcgcatcat cgcaggtaca gtcaagactg
     2161 atccactcga atccttccaa tggtgcatga ccggtctcaa gatcaatgcc gagaaaccca
     2221 tgaaccggcc aatcttcacg gccggtctcg ctccgctgga tatccctgta ctgcgaaaca
     2281 gcctcgatcc cgcagccacc cagacctatg acaagcgtcg gtacaaagat atccgccagt
     2341 gcagtatcaa gattcatatt tgtatccatg aaaaattgac atgcaccaca tccgtaacga
     2401 tccattttga aatttccggt tccagggaaa gaacgtagtc aaacacagcc aaagtgtata
     2461 acagcgaaag cagaaacaag acccatccag cgagattgat ccctgcgaat accctgttct
     2521 gccgtgcacc gactttttcc tgaaccgtgt ctttacgact tttcaattcg gcccgtaaat
     2581 tgaaccgtcc atgtgtcttg taatatgccc tttcaaaaat cctcgatacg taccagtgaa
     2641 acgcgtacac gaatgaagcc aaaaccacaa cgatcaaaaa agaggaaaag aagggatatt
     2701 ccttcctgta cattgcaaga agtacggcac acagcatcga gacagacata caaagcattg
     2761 ctgccacatg tggcatattg catttaaggg catacagaaa actctgtgag catatttttt
     2821 cgtttctccg ggccggcctg atgagcagga gcacatcaat atgccgatgt tcctgttgct
     2881 catccatttg tggcactctc gtaatgtttt ttgattttgt gttgttcaaa atggagtatg
     2941 atctcgatcc gcctgttgag cgcggcaagc tcatcgataa taccacgtcc gggtcttctg
     3001 tcattggtca cggacatgta ctggctgtag cctttggcgg acagaagtgt cttgcaatct
     3061 tcttgcggat gcagcccctt ctcaatcatc agtttgacga cagatatggc cctgtcagtg
     3121 gacagctccc agtttccata gatcttttcg tttacagagg ttccggaatc ggtatgccca
     3181 accacctgaa tatcgggcat gacctctttc acgcggtttt tatagctcgg cagaagaatc
     3241 tcgttgatca cagtttcgag aattcgtctg ccctccggtt tgaggacctt tttatccttg
     3301 tcaaaaagga tatccgcacc aaacgtcatc atgatgtcaa atccatcccg actcgtcttg
     3361 atcccgctgg atttgttttc atctgacttg atccgtgttt tattgatgtt cgagacactt
     3421 gtctcgacac acatgtgaaa cttttttaca taatcgattt ctttttcacg cttgacaatc
     3481 atgatgatga tgacgaccat cagaatgaaa aacagggata tggccacatc gacaaaagat
     3541 ggccagaaat ttccggattc tctgcgcctg cccctcatca tctatgtgcc tttttgacat
     3601 tttcaatatg gtccacccaa tcccttgtcc ccaacacaat cttgaaatcc tccagggcct
     3661 gggccacatc cttgtgccac ttgctgaata cttcaacaaa agacttcacc ttgtcggatt
     3721 caagtttagc gacatctgcc cagcctgtca ttttatcctc aaaacttccg acggcaccga
     3781 taaagacttc ctgtccatcg tggacagctt tgctgacagt ctgtatctcc gaaaccatcc
     3841 cgtccgtgat ttctttcaat cttccctgga attcctggat gacggaagtt gtactgtcct
     3901 tcaaggttcc ttcgatgcca tcgaacttgt caccaagacc ggcaaactgt tcggaaacat
     3961 tttttgcaac aacctcgaac ccgtcacgga cactgcccgc aacgcctgaa agactttcat
     4021 cgaccctggt ggcaactgtc ccaagttgcg cagtcaactc tcccgccagg cctttcatgt
     4081 tttcattgag ggattctttg aacgaatcct gcatggcgac gattgaatcc gaaagcccgc
     4141 ccagtctttc tccaatagtc acagccatct cgtcaaagac tttttcgatc ctgctgctca
     4201 aggttgaaac cgcggcatgc aattgctgtt cgttctctct cgaagctttc atctgctcga
     4261 caagagccgt attggattcc accatttttg ccatatctgt tgtctggctg gaaaactggt
     4321 cccggacaag cgtctggaaa tcgcccgtac tcctggccat ttccgttaca gtgttgttca
     4381 tgaatgtcac aactcgtgtg aaatcgttga aggcatccgc cgtggaatct gttctggtct
     4441 tgaaatcgtc catagtcctt ctgaaagtcg cagccgattg tgaaaaacgc aattgcacct
     4501 cacgctggtt ctgtgcctgg gcgcgatact cgcgtgtcaa cccgtcgaaa ccctcaattg
     4561 ccgagagaaa gccctccttg atttcaagga gcagactctt gtcgtcagcc gggcgatagc
     4621 gtggaagaag cctggaacgc aggacaaatt caagtctctc aagtgctttg tgttgtcgac
     4681 cacgaacggc atgcaacaac tttccgaaaa aaattgttac ggctatgccg gcgagcgttg
     4741 taccaaacgc acccttcatt tggctgaaga caggactgaa tgcacccaca gccgctgtat
     4801 cactttcaag aatgcccgac atttgaaata cggaaccatt caacccaata actgttccca
     4861 aaagtccaag aagaacaaag agtccgagat aatgccacgc cttgtccgcg tcggagccaa
     4921 atttgtcatc gatcatggtg cgtacatcca tgccctcgat cttcccgcca ttttcatgaa
     4981 tcacacaaag ctcttcaaca agctcccgta cctcgctgga atcaatgcca tcaagcgcct
     5041 tgttgctcac atcacgcaca gtgttacttt tcgccttgat tttatcaata ttattttcca
     5101 gtattcccga tgattcggca acgctactgg cttgaagcat tgaaatccga acaatcatgt
     5161 aaatcctgcc agcaaatgca agaataaaga agtaggctac caggaacaat attgattcca
     5221 gatcaaattt ttcgggtatc gcatgcacca tgagccatac gacgacacag acggccacga
     5281 tcgaccaacc aaaccaaatc cataaattgt tgccagtatt catttttttc ttcatgcctg
     5341 atcctttgct tgtatcgtat ccgggaccaa cagggtaaag cttgcaacaa gttcaggccc
     5401 cttcccggtc ggctttgaga ccgcccggat gatggctgta cgtacggtat tgcctgtcaa
     5461 tgacttctga attttcagac tccatgtatt cctgtcatct cttgccctgg agatgcttac
     5521 acctgtctgt tcacggatct cttcgatgga tccggcgggt gtgtccgcaa ccccatcgag
     5581 aagctcccac accacatctc catgcggcaa attgtcttcc ctgtcgagtg ccatgacaaa
     5641 ttccccggaa gacaaaaggc caggcgagtc aggatgttcg acaaaacgta aaaaatgtga
     5701 tcgaccatac cctctcggct caacaatcgc aaaatccaac tcaacaagct ctgcgtcaag
     5761 ctgtacagtc tgctcgaaaa aatttccggt tttggagaca gccgttacaa tgcagcggac
     5821 aacatccaca ggatgcatga gtccagtatc catctcgcat tcaagggtat gccactcaat
     5881 tccgccacca ccctttccga caacaggact tggccaacga tgtctgaaga gatcatatcc
     5941 gatttcaata tccagctccc ctgatcctgt cgtcgggacc atcaccagta ttttgacttt
     6001 ttctccaatt gcgatatgcg aaggagcaag tatcctgacg ccttcgagca tacggatggt
     6061 cgatgcacca cgttgcaatg gaaccggtat gcttgtctca tttatgccat cactgacacg
     6121 gatggccaaa tcctttgccg tgtctcgctt cattgcggac tcaagaacag aaaagcggat
     6181 gcgtgcaacc tttccgggcg ctattgaaca ggaatcggtg tgcagtttca tgaatcgacc
     6241 atcacaccgc aaggtcagcg tgacggatga atccgcaacc gcatagagat tgagatcacc
     6301 cgtagccaca gtccgtcttc ccgggttgca cacaggcgcc atcagccgtg gcccatccag
     6361 accgggcatg atccaggcct ccgttgccac tccggatgga tccgcactgt tgaccgagag
     6421 ggatctgtat tgcccctgtt cgtcttcgca gtctgtaacc aacaccctgc tccaacccat
     6481 tgcctctata cgttccgaaa ttgcatatcg cacaagactg cggccggttt cggtgttctc
     6541 aagtaccttc ctcggaataa tggccgcaga atcggacaca acaagtccgc cgtttctgct
     6601 ttcatataaa acaggcgtcg cttcaatctc gttttcccga agcatgagtt catgctgaaa
     6661 agattgttct tcgggcaaaa agatcgttga tggagaaagt tcccaggaga agagtcgttc
     6721 ggaaacattc cataccagcc ggccttcaac cgagtccgaa agagatccat tatatttgaa
     6781 agaaatctcg atatcctgtt gactcaacaa accggattct gggcggcatt cgaaaacaag
     6841 cgaaacccgc ttcccggatt tgatggtaca tggcattccg gtattgcaga tcacaggcgg
     6901 atccgatttt tttttccatt ttcgggatga taccgccacc tgattgacaa cacagtcctt
     6961 ctgcccgacc gcaaagactt cgagcaaaaa cgacatcccg gttttgctcg acacttctct
     7021 ttgaaaagta tccgggagaa tgagaagcgg ttgaatatac cgggaaaatt ttgaaagatc
     7081 ttcacccgta tcaaaaactc tcaggtccaa catctcagcc agcgggatcc gttcactcgc
     7141 ccggctctgg ttgatggttt gggcatcggt cacggtttcc gtcgcatggg gttgttcacc
     7201 tgtctcgggc agaacaggaa ccactaccgc cccgtttgtg tctctcgtga ccgtcagacc
     7261 ggaatccggt tcaccatcat gaacaagtgc cgatccattt tcatccacat gacccgattc
     7321 atccggaagg attcgttctg ttccggtatg cccatcctct tgaccagaga tcgccgaagt
     7381 ctcccccggt gtctcctctt cgaccaccgg gttcctgttg tccacgcttt cgttcgcgcc
     7441 catcgtatcg tgatcctggg ttgaaatcgt ctcgctggat gtgtccgcag cagtatccgg
     7501 aacgggagca tctgtctccg ttttctgatc atggagactt ttgtccggat tctttttcat
     7561 gatccatctc atgattcatg cctcgctacg atgacttcag ccttggcaac gacacggcct
     7621 ctgtatcgta atcccggttt tttacaacga acaatcctgt tttcgccaac acctggctca
     7681 tcgacatatt ctatcgcttc ctgacgcaaa gcattgtaaa tctcgccaac acccggattg
     7741 atcagttcta tctcatgtct cgcaagatat gtctgaatat cggttgatgc ccccgcatca
     7801 gcacttttct tttgagcact caggtcgtcc aaactctgtg cttcattctt cgtgaccgta
     7861 tttttggtac ggaacatctg ctcaagcaat cctgcattcg ctttcatcat ggtacgcaat
     7921 tctccgagtt cctgttgcat actctcgcgc tgactgccca tctccctgcc gagagcactg
     7981 acaacctggc caagactcgc catttcctgc aatgactttc caaatgtgga gttacgcccg
     8041 tcggatgcat catccccttg acgactgaga tagcgcagca gattgaggat ttcctgcata
     8101 ctgtttttga ctttgacatc catgaagatc aataccatca gtattgaaac cagtccaccc
     8161 atgacgagcc aggtgatcat cgagacttcc tgattttgaa ccggcggaac agtcctggca
     8221 ggaacactct tgatctggtc agtcacacca atgtttgttt tttttaccgg gcttccatca
     8281 aggcctgaca caatccctga caaaaccagt accaggaaac atcccaaaaa ggctgatttc
     8341 atgttctctc ctcaatcgac cggatgcgga atttcgatat tcatgaccga atgcgttcca
     8401 ctgtcctttt tattccctgg ttcaatcatt tcggtaaaga gtctcgcagt catgccatca
     8461 tgcaactgga gaccgatatc aaaatgcaca ttcatgtttt tttcgatcat tctgaccttt
     8521 tggtccttgt tcagccccgg gctgcagatc caggcaggat atttttgatc cgggcaatag
     8581 cgtgctgaaa ccggaaagta aaattccgac ggagccgaca cgaccgccac ccgctcggca
     8641 tgaaaggccg gatatggacc tgacgctggc cagacgattt ctccgattcc cggaagtccc
     8701 aagacaagtt cggtctgata tctgaatttg acaggattgg tcgcctgggc tgagagcgca
     8761 tcagcgatat gtttgacatc gagcggatcc atcccgaaca aatcgtttcc ttcgatattg
     8821 gggcccggct ttccgacaat cttcagacct tggccggcac tcttgaactt gcgcgtcatg
     8881 tcaaccgatg ccagccgcca tgcgacatcc tcacgctgat acctgatatg catgacaccc
     8941 gtggcatgct cggaaacgca cagtgcatcc tcacgaattc catgatattc gagaaccgcg
     9001 gcgccggcca caatatcggt cataatgctg cagtcgccaa atcgtcctgt gttggcgact
     9061 gcctggcgca agagactgcg tgcggcactg ctccaatacg agggaactat gcagatcagg
     9121 gaccctgcac cctccggcgc aatctggagc aggaactcct caagccactg tttgctcagc
     9181 tccatgcgtt gcgcccatgc gcctggatca cgtggataca gggatgcact ctcacgaaca
     9241 aatctcgaaa gctcggacag atcctctgaa agtgccgtat tgggcagggt ccgtggcagt
     9301 agactgagac aggcaacatc cagccgacgg accaagactg cattctcgtt catgattgct
     9361 gtatcctttg caatggcagt tcccgcatct cgcctgtatt cgtcaattcg atcaccaatc
     9421 ttggtacttc aacctcgtcc tgatactcga tccagaggtg gtgccgtgtc acaagccaat
     9481 caccatcatc actgaaagcc gcatcgggaa ccgtcgagag atcggctgaa aaaaaaccaa
     9541 aatctttaga ctcggaaagc acatctcccg ggctgcctgt cccttcagcg gagtgcagat
     9601 caaaacggta tctgttaaga ttatcaaaat tgattggctt atcaaaaaag gtgtattaga
     9661 ttatcaaacc gctctcgaa
//
LOCUS       DAOSHE010000024         9663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6008_length_9663_cov_4.612198, whole genome shotgun sequence.
ACCESSION   DAOSHE010000024 DAOSHE010000000
VERSION     DAOSHE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_9663_cov_4.612198"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLD82_01385"
     CDS             complement(<1..1033)
                     /locus_tag="PLD82_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG64070.1"
                     /translation="MPEGMFRNYRKRLSDLLKITEDLNHVKDIDALLDKILYEARQFT
                     NADAGSIYLREGDQLRFSYVQNDTLAGRDPAANKHLYSTQSITINDASISGYVALTGR
                     PLLISDVYDLGEDVPYRFNRSFDEKSNYRTKSILTVPLVTSRQDVIGVMQIINSRNKN
                     GVIKAFSRNDMMYVNFFANNASVAIERAKMTREIILRMIRMAELRDPKETGNHVNRVS
                     AYAIEIYEKWAQNKGIERTVIKKVKDTLRISAMLHDVGKVAISDTILKKPGKLTEAEY
                     AVMKTHTLSGARLFDEAASDWDALAAEVALTHHERWDGTGYPGNIEDVWSENHVLSKG
                     LAGEDIPISG"
     gene            complement(1149..2024)
                     /gene="lgt"
                     /locus_tag="PLD82_01390"
     CDS             complement(1149..2024)
                     /gene="lgt"
                     /locus_tag="PLD82_01390"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG64071.1"
                     /translation="MERKNWLALLPLAGTMVLATVVALIASGNGPFIHDISPVLLSLG
                     PLQIRYYGLVYMVGFLLVFYLLRRAARNKTLALNEDELERFVLLLIAGVVVGARLFEV
                     VIYNPGHYFSNPLEIFEVWKGGLSFHGGLVGVLFVTWLFSRKKGRPSFFTLTDMLVLP
                     ALAMLAFGRLANFINGELYGGVTDVAWGVRFHGAEGYRHPTQIYESFKNAFSFGILFF
                     IHSFKPRTGVLTSAFLIAYGSFRFVIEFFKDYGEYGYKTISIDALNVAHLLCLTMILL
                     GVVGLVLVRRRGKSR"
     gene            2274..2675
                     /locus_tag="PLD82_01395"
     CDS             2274..2675
                     /locus_tag="PLD82_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG64072.1"
                     /translation="MKALPMYTGLIWIVASLLAGGCSSKSGTGNDNAPLLSYSQLTNR
                     LASTTNAILLDIRRDDEVARGMIPGAKHIPVEELRQRIHEIPTNSTVIVYCASGKRVP
                     KALQLLKAAGCTSLFNFVSVTNWKGKLAMPR"
     gene            2659..3291
                     /locus_tag="PLD82_01400"
     CDS             2659..3291
                     /locus_tag="PLD82_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64073.1"
                     /translation="MQCPAELLDDLAGLYRDIPVLTKELPGDATLWCIGKRKADIIRP
                     IQAVKPDIRPVLIGKERKELFLERDRLAELPSMGFLRDIETPGWTVGLPYPVHAVSLG
                     FRLSSLNDDTRRECLKDLSDLLVMGGLVLWSDFFLPQTLEIHATYLDEYTRIASIDPL
                     DAGRFRETFLEHHPNPVLLETAVNEVYAAGFANAEIVAKRMGIAVLVARK"
     gene            complement(3316..4419)
                     /locus_tag="PLD82_01405"
     CDS             complement(3316..4419)
                     /locus_tag="PLD82_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64074.1"
                     /translation="MAHIACYLSSHGYGHAVRSAAIIDEIARCRPDARVHVITGAPCF
                     LFAQVCRERKNVHLRSVATDFGLVQKDPRFFSLQETAERLESLITDAGSLIEREREFL
                     VRENIAAVWCDLPFLPFESARREGIPAVGLGNFSWDWVYEYYRSVHPAFGHAAELAAA
                     CYQTAGLYLKLPFSPPAPAFPLQEEIPLACRSPRLERAEVRRMLKLGDSQRVVLIAFS
                     GLSLSASARARLESIPETVFLVPAPMQLEISNGISVPAGEPDFASLCLAADVLVTKPG
                     YGIVTDAITCDTPIVYTDRGDFPEVPWLEQLIRHSLGGASLAMTQFEEGDWEGALQAA
                     VDALNKKRQDGQSRMRTDGTPLAAQRFLGFCGA"
     gene            complement(4425..5117)
                     /locus_tag="PLD82_01410"
     CDS             complement(4425..5117)
                     /locus_tag="PLD82_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64075.1"
                     /translation="MFRILLVLFMLLFNIGLLAQAGAASGSETNAKAVGIRYTNESDT
                     WRVSLVRFIMKGETAKYGEVEAAMKQFTAVTTSYIVYKRPGHEMAQELYYRNLENITR
                     IEQLKSLVEMTHRDNPESTKDFFSKYSLTDADFSGFDAFEKAYDRIMGRWRGEFEAAM
                     QKRSVAELKVLREDMKLLQQAVSAFVLLPGKSSNKVPQLQMMENELRHGIVMLQMYAE
                     TIQQAALPAGGR"
     gene            5292..6524
                     /locus_tag="PLD82_01415"
     CDS             5292..6524
                     /locus_tag="PLD82_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64076.1"
                     /translation="MPTRSGNNQTSAGSGSWINLPVRLFLTDLGETAMQDKSRITIVP
                     DAAGNELPSINGERLLASTLKSFIINSRVNQIELSRAELISKRHEITDITKLIVYSLL
                     YRLFPINLVEALDARGIHIMSEDPATDRIWMEKEREKQRALRLEIFAKSREIQSRERL
                     SAEKDPVYQKKSIASITVKLIDMIPIRVWRAIAERDEDRKKIIECILVLLGNYLNRSR
                     ISDHLSAAFLEWVQNAEKINLKHAFSLWSKEYEAREGHPHHFSTVDQALLEDKQYRDV
                     LTKLAEQHHITLKINWKFGRSRHGRGTDPASIADLAVVRIRLVNKGLIGDWVRKNIEI
                     KINTSTREKHLGEFWQPPQDDRQLGSGLGLIYTAYLKNECQRQGIDVFVQTREETHSE
                     ETIMTLTLKINPTGLTPG"
     gene            6638..7165
                     /locus_tag="PLD82_01420"
     CDS             6638..7165
                     /locus_tag="PLD82_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64077.1"
                     /translation="MAKSASVHEPKGESEIVRFHCRMEPSIRAWYCSSDTGINNACHE
                     ALMQFANGIKGGTIRLDVKSRFLERTDKSQKVECILQLSCFEKDWLQKMAFKLHTSQT
                     EVLRMALEWWMEAKNSVGGGSATRRARKKWHHGVITLRPYAVRYNLWGQGIVTMNNFP
                     TQQDAEKGLQAISTA"
     gene            complement(7322..9445)
                     /locus_tag="PLD82_01425"
     CDS             complement(7322..9445)
                     /locus_tag="PLD82_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOG64078.1"
                     /translation="MIEKTYAKPDMTEVFGSHVFNDRVMRERLPKDTLKELRKCLAGE
                     ISLSPELADVIANAMKDWAIERGATHYTHWFHPLTGTTAEKHDSFISPTDDGGVIMEF
                     SGKQLTQGEPDASSFPSGGLRATFEARGYTAWDITSPAFLKEDSAGNLTLTIPTAFCS
                     YNGQALDKKTPLLRSMRAVSEQAMRVLRALGNKTAKKVVPTVGPEQEYFLIDREYFLQ
                     RLDLITSGRTLFGAQPPKGQELEDHYFGSIKDRISEFMVDLDNELWKMGITSKTKHNE
                     VAPGQYEMAPIFATTNIATDHNQLVMETMQKVALRHNLVCLLHEKPFAGINGSGKHNN
                     WSLATDDGMNLLDPGHTPSENQQFLIFLSALIRAIDRHADILRATAASTGNDHRLGAN
                     EAPPAIISIFLGEELSEMFSALAKGVKYESRGQSYLKVGVDTLPSLPKDNSDRNRTSP
                     FAFTGNKFEFRMVGSSASIAGPNVAINTIVAEVLDGIANRLEKAADKAAESQAIIREI
                     ANEHHRVIFNGNGYSREWVLEAEKRGLPNIRNTVDALKAFVSERSLTLFEKYRVLTRE
                     EMHSRYEIYVEQYAKQINIEGRTAVNMCHKQYIPAVLEFVHGLAETVTRIKQAGAYDA
                     SISEALLGKALAKLSSADSNVRDLEAALDKAAAEMDVTRKAAICRDEVVVALFALRMD
                     IDALEAIMPKDLWPVPSYEDMLFKL"
BASE COUNT         2476 a         2697 c         2349 g         2141 t
ORIGIN      
        1 gcccactgat ggggatatcc tcaccggcaa gccccttgct caagacgtgg ttctcgctcc
       61 agacatcctc gatatttccc ggatatcccg taccgtccca tcgttcatga tgcgtcaaag
      121 caacctcggc ggccaacgca tcccaatcag aagccgcttc gtcaaacaga cgtgcgccac
      181 ttagcgtatg tgttttcatg acagcgtatt cggcttccgt cagcttgccc ggctttttga
      241 gaatcgtatc cgaaatggcg accttgccga catcgtgcag cattgctgaa atccgcaagg
      301 tatccttgac cttcttgatg acggtcctct cgatcccttt gttttgcgcc cacttctcgt
      361 aaatctcgat cgcatacgcg ctcacccgat tgacgtggtt tcccgtttcc ttgggatcac
      421 gcagctcggc catccggatc atgcgcaaaa tgatctcgcg ggtcatcttg gcgcgctcga
      481 tagctaccga ggcgttattg gcaaaaaaat tgacatacat catgtcattt ctggaaaacg
      541 ccttgataac cccgttttta ttccgggaat tgatgatctg catcaccccg atcacatcct
      601 gccggctggt gacaaggggg actgtcaaaa tggattttgt ccggtagttc gacttttcat
      661 caaaactccg gttgaatcga tacgggacat cctcgccgag atcatagaca tcactgatga
      721 gaagcggtcg cccggtcagt gcaacatacc cggagatgga cgcatcattg atggtgatgg
      781 actgggtcga gtagaggtgc ttgttggcgg caggatcccg gccggccaga gtgtcattct
      841 ggacataact gaaccggagc tgatctccct cgcgaagata gatggacccc gcatcggcat
      901 tggtgaactg tctggcttca taaagaatct tgtccagaag tgcatcaata tccttgacgt
      961 gattgagatc ctcggtaatt ttgagcaaat cactgaggcg tttgcgatag ttgcggaaca
     1021 tcccttcggg catgacatac gcacctttac tgatctcttc tttccagtat cgtcacctgc
     1081 gaaggcaaaa tcgagaaaaa cccgcgctgg tccccggcgc agcagagaaa gtgcgtaggg
     1141 gctggagctt accgggattt tccgcgccgc cgcacaagaa ccaggccgac cactccgaga
     1201 agaatcatgg tcaaacagag aaggtgggcg acattcaggg catcaatcga gatggtcttg
     1261 tacccgtatt caccatagtc cttgaaaaat tcgatcacaa agcggaatga gccgtaggcg
     1321 atcagaaagg ccgacgtcag aaccccggtg cgcggcttga aggaatgtat aaagaagaga
     1381 atgccgaacg aaaacgcatt tttgaaggat tcatagatct gggtcgggtg acggtagcct
     1441 tctgcaccat ggaaacggac accccaggcc acatctgtga cgccaccata gagttcgcca
     1501 ttgatgaaat tggccaagcg cccaaaggcc agcatggcaa gcgccggaag gaccagcatg
     1561 tcggtcaggg taaaaaagga tggccgcccc ttttttctgg aaaaaagcca ggtcacgaag
     1621 agaacaccga ccaaaccgcc atggaacgag agccctccct tccagacctc gaagatttcg
     1681 agcggattcg agaaataatg cccgggattg tagatcacga cctcaaagag tctggcaccg
     1741 acgaccacac cggcaatcag caagaggaca aaacgctcaa gctcatcctc attgagagcc
     1801 agcgtcttgt tgcgcgccgc ccgtctcaag agatagaaga caaggaggaa cccgaccatg
     1861 tacacgagcc cgtaataccg gatctgcaac ggtccaagcg atagcaggac cggactgata
     1921 tcatggataa aaggcccatt ccctgaagca atcagggcca ccacggtggc gagcaccatg
     1981 gtgccggcca aaggcagaag agctagccag ttttttcgtt ccatctccag aaacctcctt
     2041 gtacgatcga cagagcctgt ctggacaggc ctctgaatac taaccaaaaa accatggata
     2101 tgcgccgttt caaatcctct tccggggggg agattcagtc cggtgtgatg gcacagggag
     2161 atgtggtatg gcatttcccc tgttggagca accagccagc gcccccttgc cagagaggac
     2221 cagacggtct atcatgtcca tcagacaata ttcgatacaa agagaggcat acaatgaaag
     2281 cactgccgat gtacacgggt ctgatctgga tagtcgcaag cctgctggcc ggaggttgtt
     2341 catcaaaaag cgggacaggc aacgacaacg cacccttgtt gagctacagc caactcacaa
     2401 accggttggc ctcgacaacg aatgccatcc ttcttgacat ccgccgcgat gatgaggttg
     2461 cccgcggaat gattcctgga gcaaaacaca tcccggtcga ggaactccgg cagcgaatcc
     2521 acgaaatccc gaccaacagc accgtcattg tgtactgcgc aagcggcaaa cgcgtcccga
     2581 aagcacttca actgctcaag gctgcaggat gcacatccct cttcaatttt gtatccgtca
     2641 cgaactggaa gggcaagctt gcaatgcccc gctgaactcc tggatgatct ggctggtctc
     2701 taccgtgaca tccctgttct gacaaaagaa ctgccgggag atgcgacact atggtgcatt
     2761 ggaaaacgaa aagcggatat catccgaccg atccaggccg tcaaaccgga tatcaggcct
     2821 gtcctcatcg ggaaagaacg gaaagaactc tttcttgagc gggacagact ggccgagctt
     2881 cccagcatgg gatttcttcg ggatatcgaa acacctggat ggactgtcgg actcccgtat
     2941 ccggtccatg ccgtcagtct gggtttcagg ctttcttcgt tgaatgatga cacccggaga
     3001 gagtgcctca aggatctctc ggatcttctg gtgatgggtg gacttgtcct ctggtccgat
     3061 ttttttcttc cgcaaacgct cgaaatccat gcaacctatc tggatgagta cacccggatc
     3121 gcctccattg acccgctcga tgccggccgt ttcagggaaa cattcctcga gcaccatccg
     3181 aatccggttc ttcttgaaac cgcagtcaat gaggtctacg cagccggttt cgccaatgcg
     3241 gaaatcgtcg caaaacggat ggggattgcg gtcctcgtgg cccggaaata atacccggcc
     3301 aaacaatgca gcctatcagg ccccgcaaaa cccgagaaat cgttgtgcgg ccaaaggtgt
     3361 gccatcggtc cgcatccggg attgaccgtc ctggcgcttc ttgttcaaag catccaccgc
     3421 ggcctgcaaa gcgccttccc aatcgccctc ttcaaattgc gtcattgcca atgatgctcc
     3481 accaagagaa tgccggatca attgttcaag ccagggcact tctggaaaat caccgcggtc
     3541 cgtatacaca atcggcgtgt cacaggtgat cgcatcagtc acaatcccgt acccgggttt
     3601 tgtcacaagc acatcggcgg ccaaacacag ggaagcaaaa tccggctctc ctgccggaac
     3661 agaaatgccg ttggaaatct ccagttgcat tggggccggt accaaaaaaa cagtctccgg
     3721 gatggattca agacgggcac gggcggaagc ggacaggctc aacccgctga aagcgatcaa
     3781 caccactctc tgcgaatccc cgagcttgag catccttcgc acttccgctc tttcaaggcg
     3841 cggagacctg caggccaggg gaatctcctc ctggaggggg aaggccgggg caggcggact
     3901 gaacgggagt ttgagataca agcctgcggt ttggtagcac gcagcagcca actccgcagc
     3961 atggccaaac gcaggatgga cagaccggta atactcatag acccagtccc aggaaaaatt
     4021 ccccagacca acagccggga ttccctcccg ccttgcagat tcgaagggaa ggaacgggag
     4081 atcacaccag acagcggcaa tgttttcacg aaccagaaac tctctctccc gttcaatcaa
     4141 gctcccggca tccgtaatca aggactcaag ccgttccgct gtctcttgaa gtgaaaaaaa
     4201 acgggggtct ttttgaacca acccgaaatc cgtggcaaca ctcctcagat ggacattctt
     4261 tcgttcacgg caaacctgtg caaagagaaa acacggggcg cccgtgatga catgaacccg
     4321 tgcatcggga cgacaacggg caatctcgtc aataatcgcg gcagagcgca cggcatgtcc
     4381 gtagccatgg gaggaaagat aacaggcgat atgagccatg agctttatcg gccacctgcc
     4441 ggcaaagccg cctgctggat ggtctcggca tacatctgga gcatgacaat cccgtgtcgg
     4501 agctcgtttt ccatcatctg cagctgagga actttgttgg atgactttcc tggaagaaga
     4561 acaaacgcgc tgacagcctg ctgcaggagt ttcatatcct ccctgagcac cttgagttcg
     4621 gctacggatc ttttctgcat ggccgcttca aattcaccac gccaccttcc catgatgcgg
     4681 tcatacgctt tttcaaaagc atcaaaaccg gaaaaatcgg catccgtgag agaatacttg
     4741 gaaaagaaat ccttggtaga ctcgggattg tcgcggtgcg tcatctcgac aagactcttc
     4801 agctgctcga tccgggtgat attctcgaga ttcctgtagt acaactcctg cgccatctca
     4861 tgcccaggac gcttgtatac gatatacgag gttgtgacag cggtgaattg cttcatcgct
     4921 gcttcgacct ctccgtattt ggcagtctcg cccttcatga tgaagcggac aagagaaacc
     4981 ctccaggtat cactttcatt tgtatatcgt attccaaccg ctttggcgtt ggtttccgat
     5041 cctgaagctg ccccggcctg tgccaataaa ccgatattga acaacagcat gaacagaaca
     5101 agcagtatgc gaaacatttc cacctccatg tatgcaaggg tcatcctctc cttcgcgatc
     5161 catgctccgg acatacacca ggaagacctt tctttctatc ggccaacagg gcggagaatt
     5221 caaatttctt ttgagcgtat aaaaataatg acgataggta tacatacagg gaaaaaccag
     5281 gagagatcat catgccaacc cgctccggaa acaaccagac cagtgcagga agcggttcct
     5341 ggataaacct gcctgttcgc ctgtttttga ccgatctcgg tgaaacagcg atgcaggaca
     5401 agtctcgcat caccatagtg ccggatgctg ccggaaatga acttccgtcc atcaatggag
     5461 aaagactcct ggccagcaca ttgaaatcat tcattatcaa cagtcgtgtc aaccagattg
     5521 aactctcccg ggcagaactc atctcaaagc gccacgaaat caccgacatc accaagctca
     5581 ttgtctactc ccttctctac cggctcttcc cgatcaacct tgtcgaggct ctcgatgcac
     5641 ggggaataca catcatgagc gaggatcccg ccactgaccg gatctggatg gaaaaagagc
     5701 gggaaaagca acgggctttg cggcttgaaa tttttgcgaa gtcccgggag atacaatcac
     5761 gggagcggct ctccgcggaa aaagaccccg tctatcaaaa aaaatcgatc gcatccataa
     5821 ctgtcaaact gattgacatg ataccgatcc gtgtctggag agccatcgca gaaagggatg
     5881 aggatcggaa aaaaatcatc gagtgcatcc tcgtccttct cggaaactat ctcaacagaa
     5941 gcagaatcag cgaccatctc tcggcagctt ttttggaatg ggtccagaat gccgaaaaaa
     6001 tcaacctgaa acacgcgttc tcgctctgga gcaaggagta tgaagcccgg gaaggacatc
     6061 cccaccattt ttcaaccgtt gaccaagcac ttctggaaga caaacaatat cgtgatgtgc
     6121 tgaccaagct cgcagaacag catcacatca cactcaagat caactggaaa ttcggccgat
     6181 cccgccatgg ccgcgggaca gatcctgcaa gcatcgcaga tttggccgtc gtcagaatcc
     6241 gtcttgtcaa caaaggattg attggagact gggtccgaaa gaatattgaa atcaaaatca
     6301 acaccagtac ccgggaaaaa caccttggtg aattctggca gccacctcag gacgaccgac
     6361 aattgggatc cggactcgga ctgatttaca ctgcctacct gaaaaacgag tgccagcgcc
     6421 agggaataga cgtttttgtc cagacacggg aagaaacgca ctccgaggaa accatcatga
     6481 cactcacact gaagattaat ccgacaggtc tcacacccgg gtgaaacttt tcacgcacca
     6541 tagtgcatga accaagggta aaaaattttg aattttcagg gaacctgcgg cacctcccgg
     6601 acctctcctt actgtgagag acacaaggga ggcccgtatg gcgaagtccg cttctgttca
     6661 tgaaccaaaa ggcgaatccg agattgtccg cttccattgt cgaatggaac catcgattcg
     6721 tgcctggtat tgcagctcag ataccggtat caataacgcc tgtcatgagg cgctcatgca
     6781 attcgcaaat ggcatcaaag gaggaacgat ccggcttgat gtcaaaagca gatttttgga
     6841 gcggacggac aagagccaaa aagtcgaatg cattcttcag ctctcgtgtt ttgaaaagga
     6901 ctggctccaa aaaatggctt tcaaacttca tacaagtcag accgaggttc tccgaatggc
     6961 tctggaatgg tggatggaag cgaaaaattc tgttggaggc ggatcagcaa cccgtagagc
     7021 acgaaaaaaa tggcaccatg gtgtgattac actaagacct tacgccgtcc ggtacaacct
     7081 ctggggccaa ggaatagtca caatgaacaa cttccctacc caacaagatg cagaaaaagg
     7141 gcttcaagcc atctcaactg cgtgatcttc atcccccgaa tcacatgcaa acactcggac
     7201 gttgacaaac atctggttca agttttctgc aaaaaactct gctccgcttc cctgagaaaa
     7261 taaaaaatgg cggcaaagac agccgtggct tctttgccgc tttctccata aaagaatcat
     7321 cctagagttt aaacagcata tcctcatacg aaggaacagg ccacaaatcc ttgggcataa
     7381 tcgcttccag agcgtcaata tccatccgca aagcaaaaag cgccacgaca acctcatccc
     7441 ggcagatcgc cgcctttcgg gtgacatcca tctctgcagc agccttgtcc agggcagctt
     7501 cgagatcccg cacgttggaa tccgcagaag agagctttgc caaagctttt cccaacaggg
     7561 cctcgctgat gcttgcatcg tacgccccag cctgctttat tcttgtcaca gtttcagcga
     7621 gtccgtgtac aaattcaaga acggccggaa tgtattgctt gtggcacata tttaccgcag
     7681 ttcttccctc gatattgatc tgctttgcgt actgttcaac atagatctca tagcgcgaat
     7741 gcatctcttc ccgggtcaaa acgcgatatt tctcgaatag cgtcagggac cgttcgctga
     7801 caaatgcctt gagcgcatca actgtattcc tgatgttcgg aagaccgcgc ttctccgctt
     7861 ccagcaccca ttccctggaa tacccgttcc cgttgaaaat caccctatgg tgctcattcg
     7921 ctatctcgcg gatgatcgcc tgtgactcgg cggccttgtc agccgccttt tcgaggcggt
     7981 ttgcaattcc atcaagaact tccgcgacta tggtgtttat ggcgacgttt ggcccagcaa
     8041 tcgaagcaga agacccaacc attctgaact cgaacttgtt ccccgtaaac gcaaagggag
     8101 aagtcctgtt ccggtcggaa ttatccttgg gaagtgaagg aagcgtatcc acgccgactt
     8161 tcagatagga ctgtccacgt gattcatact taactccctt tgccaaagcg gagaacatct
     8221 cgctcagttc ctcgccgagg aagatcgaga tgatcgccgg gggggcctca ttcgcaccaa
     8281 gcctgtggtc attcccggta ctggccgcgg ttgcccgcag aatatccgca tgccggtcaa
     8341 tggcccggat caacgcgctc aaaaaaatca aaaactgctg gttttccgac ggagtatgcc
     8401 cgggatcgag aagattcatc ccgtcatccg tcgccagcga ccaattattg tgctttccgg
     8461 agccattgat cccggcaaac ggcttttcat gaaggagaca aacaaggtta tgtcgaagcg
     8521 caaccttttg catagtctcc atgaccaact gattatgatc cgtcgcaata ttcgtcgtcg
     8581 caaaaatcgg tgccatctca tactggccgg gtgccacctc gttgtgcttt gtcttggagg
     8641 ttatccccat tttccagagc tcgttatcca ggtcgaccat gaactcggag atcctgtcct
     8701 tgatcgatcc aaaatagtgg tcctcaagct cctgcccttt cggtggttgt gccccgaaca
     8761 gtgtccgccc ggaggtaatc aaatcaagcc gctgcaagaa atactctctg tcgatcagga
     8821 agtattcctg ttcgggaccc acggtcggta cgactttctt tgctgtcttg tttcccagtg
     8881 ccctgagcac gcgcatagcc tgctccgaca cggccctcat ggaccgtaat agtggggttt
     8941 tcttgtctag cgcctggccg ttataggaac aaaaagccgt cggtatcgtc aaagtcagat
     9001 tccctgcaga atcctccttc agaaatgccg gagaggtgat atcccaagcg gtataccctc
     9061 tcgcttcaaa ggttgccctg agcccccctg acggaaacga ggacgcatcc ggctctccct
     9121 gggtcaactg ctttcctgaa aattccataa tcacaccgcc gtcatcggtc ggtgaaataa
     9181 aagaatcatg cttttccgca gtcgttccgg tcaggggatg aaaccagtgg gtgtagtgtg
     9241 tcgcaccccg ttcgatagcc cagtccttca tcgcatttgc gatgacatca gccagctccg
     9301 gcgacagcga aatctcgcca gccaggcatt ttctcagctc cttgagcgta tcctttggca
     9361 atcgctcccg catcaccctg tcattgaaca catgtgaccc gaacacttcc gtcatatccg
     9421 gtttcgcata cgttttttca atcattctat cctccatagt gctgtgtcac catacctggt
     9481 tcacgagagc cacgcttcaa atatatctgc cggcaacgaa actgaatacg tctctctccg
     9541 ggcaatccac cctccaggca ttgcattgaa ctagactgca tatggtgtgc catttttgat
     9601 tgtttttgag gaacaccttc tatcttgcac tatggtacgc agatacaccg catattcccg
     9661 ctt
//
LOCUS       DAOSHE010000025         9445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6173_length_9445_cov_4.172311, whole genome shotgun sequence.
ACCESSION   DAOSHE010000025 DAOSHE010000000
VERSION     DAOSHE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6173_length_9445_cov_4.172311"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..779
                     /locus_tag="PLD82_01430"
     CDS             234..779
                     /locus_tag="PLD82_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64079.1"
                     /translation="MALVCGLVLTGCSEDEATPLSSSSQSSLLSSLGSSTSSVSGISS
                     SSLDALSSSSSNTLSSSAAASSLSVTASSVQSSSLAVASSTSSLASTPVFTVSRGIKN
                     ASLVCRDGKAWVAWHSTNDSGFYLSQYDPAGSGWVDAGGKIANGDPSGWVAPAILADA
                     AGNVIIGTPVYIDGGTINNAV"
     gene            798..1493
                     /locus_tag="PLD82_01435"
     CDS             798..1493
                     /locus_tag="PLD82_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64080.1"
                     /translation="MTQLGGNLNTGVSRDIQLGICTNRIFALWSEIPGGMKASLSLYN
                     PGTSSWQAVGVPLFSAGGAYTPSLAFDASFRPWVAFQDYTQTPSYDATVMVFDGGWGI
                     VGGAAVNGGTVQANDTSLAFDGAGVPYLAYKNWTTFQPEIKKRVGETWTGVTIPDGAS
                     DSPQIKSFGGTIYLLAGHFDGISVWRRDGSAWVRVALAHTYAVRYTFSVDPEGSVWLL
                     YSDSSGVWYIRKS"
     gene            1580..3754
                     /locus_tag="PLD82_01440"
     CDS             1580..3754
                     /locus_tag="PLD82_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64081.1"
                     /translation="MILLVEDDPLISTLTSLLLGDFGYGVVTAGNGEDAVRKAITDER
                     VSLVLMDIDLGPGIDGTRAAQEILAKRTLPIVFLTSHAEEEMVRRVRGITRYGYVIKN
                     SGDFVLQSSIEMAYELFDAHRRIELQLEHLESSRERLDFTLAAVSDAIWDYYPEDGRL
                     YWSPRYLKMLGYGDGELTPSVETWERLVHPQDIGTAGLLFRQCVEGEIDEYDKECRFL
                     TASGEWRWVQIRGRVLSRNPDGRAVRMAGIHTDIHERKEAEEQRAVSARTLEDIIAAI
                     PSGLFIYQYDKDRGSLCLIKANKAAVAMTGIDEEGWLGREMDEIWPNQSRYGLREKYL
                     QVMVSGIPLRMDNQEYSDQRISGVYRIHAFPVPGQKLGIAFEDVTVFRRTEDTLRETE
                     TIFSEFLEHSPVYVFFKDHEIRSLRLSRNYEKMLGRPIGELLGKTMDDLFPSFLAKSM
                     IADDKRILSEGREIVVDEELGGRYYTTIKFPICLDGSPRYLAGFTIDITDRKLALDES
                     RASAGRIQKLLDEKELLLREVHHRIKNNMSTISNLLNLQADHCRTSEARNALTDAVGR
                     INTMWAVYDTLFLGKDFRFIEVRYYLEKLLEAARDSMGGQRAIEISTSLDDDQLDSRE
                     VFPLGMIVNELVTNSYKYAFVQGGGRIHLEYRRYDDGGRGIFYRDDGQGLPEAVLEGT
                     SGGFGMRLVRLLVRQLEATMEIKNVAGFTCTIRFSARPPLPV"
     gene            complement(3860..6580)
                     /gene="greA"
                     /locus_tag="PLD82_01445"
     CDS             complement(3860..6580)
                     /gene="greA"
                     /locus_tag="PLD82_01445"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG64082.1"
                     /translation="MSVDVIEKVQELLNEEKWTRATIENYSRKNFIAMDSIIETAMAQ
                     GIAKDVREICLEHMKHSQNSIIALYVIGIINYEIKALDNNYLTKAITLFRENKKWAIV
                     EFLADRILAYEENRKALKALATVYQSMNNEEELWKVWERLVKVDHDNAEIPKKIAQHY
                     QETNPDLAVYYYRMTLRRLVLHGDMDEMREIWNKLIELIPGDLDFFFSLEQKLVDRKL
                     TPEAISDHYYALYTCPCERTPDTKIAILRTVLQHHPKHKDARHALTATLREKYDGHTR
                     LDNAILISGIEMTATPVESAFEIFSKHVALDTGDAVFHRSWGIGKIASNDGQFCVIDF
                     DTKKNHRMDLEMASKSLTRLPKNHLWLMARDQPDELKNMAANDPEGFMRIVLESFGKE
                     MLLKDMKQEVVGKAKVMTESGWTSWWNRNKKIFKTNPVFGTSPEKKNVYFLREKPITL
                     EEDSLARFETEKDFVKKLLIFADLLNHAADFSTIAFQSMLGYFDEKAKSGKNDIETAE
                     AVLFLSRCLKQISARGVSAEVNEPDLKAFYTTLEDPAQTLAGLTDVENRRQLIGLIRK
                     HDSNWKEIYMRILRLPPAAYDVRVMDDLFASDYGDQVLAFFNSVVSHYRDNADRLVWL
                     AKLYFEREKREDAITLNLPDLIAAMLDIMAVANRDIQNKSNVSVNRKRYQTLNDILFK
                     ENRLERFIEEESREMVSKITGVLPVIRDAVDETELLHVESKLHEVHPGLEIGGKKQVE
                     ETRELHIFLVTRESYTQKQGEYKHIVEVEIPANSRDISLATAQGDLSENAEYKAALEH
                     QEQLKARVSALDEELKKARIVDSHDVNTDETGFGCRVTLMNLDSKSEEVLTILGEWES
                     NPQAGIISYKSPLGRSILFRKPGDEFEFQLDSKKTRYKVVNISLASVFGG"
     gene            complement(6643..7653)
                     /locus_tag="PLD82_01450"
     CDS             complement(6643..7653)
                     /locus_tag="PLD82_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64083.1"
                     /translation="MDHDGQLTFDDDPDALRMKSAINLLRLGQFAQAAAAFTAIFEHD
                     PEYPEIDTAIKAARFWENRADSITDGAPGLDNGKFLKDEWNTFEAFIGNDDRFDSLPA
                     INAIRTFVFTEAIHCYTRAWENTIAPDISLVHDIGECYFNIRDYAKAAETFEYAWELK
                     RDSSRILSRLADTCEAMAGETRELRKRALLLFREAFLHNPREIEIDRLRSHLIRHLVS
                     EVRKAGFSGEEVACWVPVHAAFEHSFSVKRELEEHEVNTLQEQSSRLEADLAKNGSNA
                     RILLPLLLNRYIWLLDHYTLQVNDAVKAELVRKRFIEHDSETWARIYTRQETHKTDTQ
                     PV"
     gene            complement(7855..8163)
                     /gene="xseB"
                     /locus_tag="PLD82_01455"
     CDS             complement(7855..8163)
                     /gene="xseB"
                     /locus_tag="PLD82_01455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG64084.1"
                     /translation="MKRDQAHPFEKGLERLEEISARLDDDAIPLEEAISLYEEGVKLS
                     ASLTEYLGKAERRILVAPQRDAEPLTIDECQPLGTEQTVTKKSVRSKKTVSREEDSLF
                     "
     gene            complement(8160..>9445)
                     /gene="xseA"
                     /locus_tag="PLD82_01460"
     CDS             complement(8160..>9445)
                     /gene="xseA"
                     /locus_tag="PLD82_01460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG64085.1"
                     /translation="SYLVPVTPLHQHSPGEAAPVLSVGQLCALIKNTLEEGFSSVLVS
                     GEISNLREYDSGHVYFTIKEAKASISAVLFAGVRRALPAGLRLKDGMKAVFAGRVSFY
                     SDRGQVQIVVSGVTPEAGEGDLYRRFEELKRKLADEGLFSESLKKPLPWYPRRVGVIT
                     STGGSVLHDIIETSAGRAPGIEILIYPSPVQGETAAAELVAALRQADRDGLCDVIIIA
                     RGGGSIEDLWPFNDESLARTIAACRTPIVSGVGHEPDVTICDFVADLRAPTPSTAALR
                     VFPERQSLADSLLSYRRRLHDGFTRRLRRARERLERQTKEALLRGIRSRIEDLTQRTD
                     TLQARISQRTRLILSEAKGRLSTLGAGLGKHDPGLPFKKGYALVRGPEGPVTSADRLV
                     TGDRIEIAFSDGRVDATVTGKKPGRPTHQMNGGQA"
BASE COUNT         1849 a         2450 c         2898 g         2248 t
ORIGIN      
        1 cagaacgggc gtctgtgacc gggaactccc gcaactctcc ggtgatgccg gacaccggac
       61 ggcatgccac tttcctcttg aataaaaaaa tattcaaaaa caccaaatgg aaatttcatt
      121 gaaatgtggt atcctcgatc cagtatccgg cctgttgccg gatgagaaaa tcggcgaatc
      181 agggggaaca gggcatggga cgtttgggca gtggtgtagc gtgcatggca ggtatggcat
      241 tggtttgtgg tctggtgttg acagggtgtt ccgaggatga ggccactccg ctgtcttcat
      301 ccagtcagtc cagtctgctc tcctcccttg gaagctcgac ctcgtcggtg tctggaatct
      361 caagttcttc tcttgatgcg ctctccagtt catcatccaa cactctctcg tcctcggctg
      421 cggcatcttc tttatccgtg actgcatcgt ccgtccagtc gtcgtccctc gctgtggcat
      481 cctcgacttc ctcgctggcc agtaccccgg tctttaccgt ttcccgggga atcaaaaatg
      541 cgtcactggt ttgcagggac ggaaaggcct gggtggcctg gcactccacc aatgacagcg
      601 ggttctacct ttcgcagtat gatcctgccg gatcaggttg ggtcgatgcc ggggggaaga
      661 ttgccaatgg tgacccgtcg ggctgggtcg caccggcaat ccttgccgat gctgcgggga
      721 atgtcattat tggtaccccg gtctatatcg atggtggtac catcaacaat gccgtctagt
      781 cctgggatgg atcatcgttg acccagcttg gagggaatct caacaccggt gtgagccggg
      841 atatccagct cgggatctgc acaaacagga tttttgccct gtggagcgag atccccggcg
      901 ggatgaaggc cagtctttcc ctctacaatc ccggaacctc ctcatggcag gcggtcggtg
      961 tacctctttt cagtgctggc ggggcctata ctccgtcgct cgcctttgat gccagtttca
     1021 ggccctgggt tgcgttccaa gattacaccc aaactccgtc ctatgatgca accgtcatgg
     1081 tgtttgatgg aggctgggga attgtgggtg gtgcggcagt gaacggagga actgtccaag
     1141 ccaatgatac gtcactggct tttgatggcg caggtgtgcc gtatctggcc tataaaaact
     1201 ggacaacatt ccagcctgaa atcaaaaaac gggttgggga aacttggacc ggtgtgacca
     1261 tcccggatgg tgcgtccgat agtccacaga tcaaatcctt tggcgggacg atctatctcc
     1321 tggcgggcca tttcgacggg atctcggtct ggcggcgaga cggatcggcc tgggtcaggg
     1381 tcgctcttgc gcatacctat gccgtgcgct acactttcag tgtggatccg gaaggttcag
     1441 tctggctgct ctattccgat tcatccggag tatggtatat acggaagtcg tgaggatgat
     1501 tgtcaaggta cgataagcgg ggcggatttc caatactaca aggtcaggca cccaatctat
     1561 ggaagaggca gcaaagagga tgattttgct tgtcgaggat gaccccctca tatcgacgct
     1621 gacgtcgctg ttgttgggtg atttcggcta tggcgttgtc acggccggca acggggaaga
     1681 cgctgtccgc aaagcgatta ccgacgagag agtgtcgttg gtcctgatgg atatcgatct
     1741 cgggcccggg attgacggga caagagcggc ccaggagata ctcgccaaac gaacccttcc
     1801 gatagttttt ctcacctccc atgccgagga ggagatggtc cgcagagtcc gggggattac
     1861 ccgatacggc tatgtgatca aaaactcggg tgattttgtg ctgcagagct cgatcgaaat
     1921 ggcgtatgag ctttttgatg cacaccggcg gatcgagttg cagctcgaac accttgaaag
     1981 cagtcgggag cggcttgatt tcacactggc ggctgtcagt gacgccatct gggattatta
     2041 tcccgaagat gggaggctct actggagtcc ccggtacctc aagatgctcg gatatggcga
     2101 tggagagttg acaccctcgg tcgaaacctg ggagagactc gtgcatcccc aagatatcgg
     2161 gacagccgga ttgctcttca ggcaatgtgt cgagggcgag atcgatgaat atgacaagga
     2221 atgccggttc ttgaccgcca gcggggagtg gcgctgggta cagatccggg gccgtgtctt
     2281 gtcaaggaat ccggacggtc gggcggtgcg gatggcaggg atccataccg atatccatga
     2341 gcggaaagag gccgaggaac agcgggccgt ttcggccagg acgttggaag acatcattgc
     2401 agcaatccct tccgggctct tcatctacca gtacgacaag gacagaggga gcctgtgcct
     2461 catcaaggcg aacaaagcgg ctgtggccat gaccgggatt gatgaggagg gatggctggg
     2521 cagggaaatg gatgagatct ggccaaacca gtccaggtac gggttgcgtg aaaaatatct
     2581 gcaggtgatg gtatcgggga ttcctctcag gatggacaac caggagtact cggatcaaag
     2641 gatctccgga gtctacagga tccatgcctt tcccgtcccg ggtcagaaac tcggcattgc
     2701 gtttgaggat gttaccgttt tcaggcggac cgaggatacc ctgcgtgaga ccgagaccat
     2761 tttttcggag tttctcgagc acagcccggt ctatgttttt ttcaaggacc acgagatccg
     2821 ttccctcaga ttgagccgaa attacgaaaa gatgctcggt cgtccgatcg gggaactcct
     2881 tggaaagaca atggatgatc tttttccgtc gtttctcgca aaatccatga ttgcggatga
     2941 caaacgcatc ctctccgaag gccgcgagat tgtggttgac gaggagttgg gcggccgata
     3001 ttatacgact atcaaatttc cgatttgttt ggacggctcg ccccgctatc tggccggctt
     3061 tacaatcgac atcacggacc ggaaactggc cctcgacgag agtcgtgcga gtgccgggag
     3121 gatacaaaaa ctccttgacg agaaggaact ccttttgcgg gaggtgcacc accggatcaa
     3181 gaacaacatg agtacaatct cgaatctcct caatcttcag gccgaccatt gccggaccag
     3241 cgaagcgcgg aatgccctga cggatgccgt cgggcgcatc aatacgatgt gggcagtgta
     3301 tgacacgctc tttttgggga aggatttccg gttcatcgaa gtgcggtatt atcttgagaa
     3361 actcctcgaa gccgcccggg attccatggg tggccaaaga gccatcgaaa tttcaacaag
     3421 tcttgatgat gaccagcttg attcccggga agtttttccg ttgggaatga ttgtcaacga
     3481 gctggtgaca aacagttaca agtatgcctt tgtccaagga ggcggacgga tccacctcga
     3541 gtaccgacgg tatgatgacg gcgggcgagg gattttttat cgtgacgacg gccagggtct
     3601 gccggaggcc gttctcgagg gaacatccgg aggcttcggg atgcggctgg ttcggctcct
     3661 ggtccggcag cttgaggcga caatggaaat aaaaaacgta gccggattta cttgcacgat
     3721 ccggttttcg gcgaggccgc ctttaccggt ttgaggttgt tttatagaac ccggccaccc
     3781 ggcttggttc gacctttcgg tgtgtcgaca aaaagggctg cccttcgggg acagcccttt
     3841 ttggaatcta accggcggtt caaccaccaa aaaccgaggc cagggaaata ttgacgactt
     3901 tgtatctcgt ttttttgctg tcaagctgga attcgaactc gtcacccggc ttgcggaaga
     3961 ggatggatct tcccagcggg gatttgtacg agatgatacc ggcctggggg tttgattccc
     4021 attctccgag gattgtgaga acttcttcgc tcttgctgtc gagattcatc agggtcacgc
     4081 ggcagccaaa accggtctcg tcggtattga catcgtgcga gtcgacgatg cgggcctttt
     4141 tcagttcttc atcgagcgcg gagacccggg ccttgagctg ttcctggtgc tcaagtgccg
     4201 ccttgtactc ggcattctcg gacaggtcgc cttgggccgt ggccagcgag atgtcgcgcg
     4261 agttggccgg gatctccact tcgacgatgt gtttgtactc gccctgcttc tgggtgtaac
     4321 tctcgcgggt gacgaggaag atgtggagtt cgcgggtttc ctcgacctgc tttttcccgc
     4381 cgatctccag gccgggatgg acttcgtgca gcttgctctc gacatggagg agttcggtct
     4441 cgtcgacggc atcgcggatg accggcagca ctccggtgat ctttgagacc atctcgcgtg
     4501 actcttcctc gataaagcgt tcgagccggt tttccttgaa gaggatatcg ttgagggtct
     4561 ggtagcgctt ccggttgaca ctgacattgg acttgttctg gatgtcacgg ttggcgacgg
     4621 ccatgatgtc aagcatcgcg gcaatcaggt caggcagatt gagcgtgatc gcatcctccc
     4681 gcttttcccg ttcgaagtag agcttcgcca gccagaccag ccggtccgca ttgtcgcgat
     4741 agtgcgaaac aacgctgttg aagaaggcaa gaacctggtc accgtaatcg ctggcgaaga
     4801 ggtcatccat gacgcggaca tcataggctg cgggcgggag acggaggatc cgcatgtaga
     4861 tctctttcca gttggaatcg tgcttgcgga tcaggccgat caactggcgt cggttttcga
     4921 cgtcggtcag gccggccagg gtctgcgcgg gatcctcgag ggttgtatag aaggccttga
     4981 ggtccggttc gttgacttcg gcagagactc cgcgtgccga gatctgtttg agacagcggg
     5041 aaaggaagag gacggcttcg gctgtttcaa tgtcattctt gccggacttt gctttttcgt
     5101 cgaagtagcc aagcatgctc tggaaagcga tggtggagaa atcggcagca tggttgagga
     5161 gatcggcaaa gatgaggagc tttttgacga agtccttttc ggtttcaaat cgtgccagcg
     5221 agtcttcctc gagggtgatc ggtttttcac gcaggaagta gacgttcttt ttttcgggag
     5281 aggtcccgaa gaccggattg gttttgaaaa tctttttgtt ccggttccac cagcttgtcc
     5341 agccgctctc ggtcatgacc ttggccttgc cgaccacttc ctgcttcatg tccttgagga
     5401 gcatttcttt tccgaaggat tcgaggacaa tccgcatgaa cccctcggga tcgttggctg
     5461 ccatgttttt cagttcatca ggctgatccc gggccatcaa ccagagatgg tttttgggaa
     5521 ggcgggtgag ggatttgctg gccatctcga gatccatccg atggttcttc ttcgtgtcga
     5581 aatcgatgac gcaaaactgg ccgtcgttgg aagcgatttt tccgattccc cagctccggt
     5641 ggaagaccgc gtctcctgta tccagggcca catgcttcga aaagatctcg aaagccgatt
     5701 ccacaggtgt cgcggtcatt tcgatcccgg aaatcaggat ggcgttgtcg agtctggtat
     5761 gcccgtcgta tttctcgcga agggttgccg tgagggcatg tctggcatcc ttgtgcttcg
     5821 gatgatgctg gaggactgtc ctcaggatgg caatctttgt gtctggagtt ctctcgcagg
     5881 ggcaggtgta gagcgcatag tagtgatcgc tgatggcctc gggcgtgagc ttgcggtcaa
     5941 ccagtttttg ctcgagggag aagaaaaagt cgagatctcc gggaatcagc tcgatcagct
     6001 tgttccagat ctcgcgcatc tcgtccatgt ctccgtggag aaccagacgg cggagtgtca
     6061 tgcggtagta atagacggcg aggtcggggt ttgtctcctg gtagtgctgg gctatctttt
     6121 tcgggatctc ggcattgtcg tggtccacct tgacgaggcg ctcccagacc ttccagagtt
     6181 cttcctcgtt gttcatgctc tggtagaccg tcgccagcgc cttgagggcc ttgcggtttt
     6241 cttcgtaggc gagaatccgg tcggccagga attcgacgat ggcccacttc ttgttttcac
     6301 ggaagagggt gatggctttg gtcaggtagt tattgtccag cgccttgatc tcgtaattga
     6361 tgattccgat gacatacagg gcgatgatgc tgttctggga gtgcttcatg tgctccagac
     6421 agatctcgcg cacgtctttg gcaatcccct gcgccatcgc tgtttcaatg atgctgtcca
     6481 ttgcgatgaa gtttttccgc gaatagtttt cgatggtggc tcgggtccac ttctcctcgt
     6541 tgaggagctc ctgaaccttc tcgatgacat caactgacat gtactgacct tcctttcaag
     6601 tgtgcaaatc cgttgcaccg gagtgcgtgt tttcggtcat gctcagaccg gctgtgtgtc
     6661 ggttttatgt gtttcctgcc tggtgtagat cctggcccag gtttcgctgt cgtgttcgat
     6721 aaaacgtttt cggacaagct cggccttgac ggcgtcattg acttgcaggg tgtaatggtc
     6781 aagcagccag atatagcggt tgaggaggag ggggagcagg atccgggcgt tcgatccgtt
     6841 ttttgcaagg tctgcctcca gccgtgagga ctgctcctgg agcgtgttga cttcatgttc
     6901 ctcgagctca cgcttgacac tgaagctgtg ttcgaaggcc gcatggacag gtacccagca
     6961 ggcgacttcc tcgccactga aaccggcctt gcggacttct gaaaccaggt gccggatcag
     7021 gtgcgaccgg agcctgtcga tctcgatctc ccgcggattg tggagaaacg cctcgcggaa
     7081 gagcaggagc gcgcgtttcc ggagttcgcg ggtttcgccg gccatggctt cgcaggtgtc
     7141 ggccagtcgc gagagaatgc gcgaactgtc ccgtttgagt tcccatgcgt attcgaatgt
     7201 ctccgcggcc tttgcgtaat cccggatgtt gaaatagcat tcgccgatgt cgtggacgag
     7261 cgagatgtcg ggtgcgatcg tgttttccca ggcccgggta tagcagtgga tggcctcggt
     7321 aaagacgaag gtacggatgg cgttgatggc cggcaggctg tcgaaccggt cgtcgttccc
     7381 gatgaatgcc tcaaaggtgt tccactcgtc cttgaggaat tttccattgt caaggcccgg
     7441 agcgccatcg gtgatcgagt cggcccggtt ttcccaaaag cgggctgcct tgatggcggt
     7501 atcgatctcg ggatactcgg ggtcgtgttc gaagattgcc gtgaaggccg cggctgcctg
     7561 tgcgaactgg ccgaggcgga ggagattgat ggcggacttc atgcgcagtg catcggggtc
     7621 atcgtcgaat gtgagctgac catcgtggtc cacggcgaca cctccccggc aatacatggt
     7681 accgaggcag gctcaccccg gagaagcccg cgtcatgcac gacggactct ctgtcaaata
     7741 taccgatgga aggatgctca tgtcaataga agaattgaaa atcaaaatat aagcgcagaa
     7801 aaacaaccca tacacattct accgagttcc tggcgggaag gcaagcggaa aatcctaaaa
     7861 aagactgtct tcctcgcggg ataccgtttt ttttgaacgg acggactttt ttgtcactgt
     7921 ctgttcggtg ccgaggggct ggcattcatc gatggtcagg ggctccgcat cccgctgtgg
     7981 tgcaaccaga atccgtcgtt ccgcctttcc gagatactcc gtcaggcttg ccgagagctt
     8041 gacaccttct tcgtagagcg aaatcgcttc ctcgagcggg atggcgtcgt catcgagtct
     8101 ggccgagatt tcctcgaggc gttcaagtcc cttttcaaag gggtgagcct gatcccgttt
     8161 catgcctgtc ctccattcat ttggtgcgtg ggtctgcccg gttttttccc tgtgacggtt
     8221 gcgtcaaccc ggccgtcact gaaggcaatc tcgattctgt cgccggtgac aagacgatcg
     8281 gcgctggtca ccggtccttc cggaccccgg acgagagcgt atcctttttt gaacggaagg
     8341 cccgggtcgt gtttcccgag tccggcgccg agggtgctga ggcgtccttt ggcctcgctc
     8401 aaaatcagcc gggtccgctg gctgattctg gcctgcaggg tatccgttct ttgtgtcagg
     8461 tcttcaatcc gggagcggat tccgcgcagg agtgcctctt tagtctgtct ctccagccgt
     8521 tcacgggccc ggcgcaggcg gcgtgtgaat ccgtcgtgca ggcggcgccg gtaactgagc
     8581 agggaatccg ccagggattg ccgttcgggg aagacccgga gggctgccgt ggaaggcgtt
     8641 ggcgcccgga ggtctgccac aaaatcacag atcgtgacat cgggttcgtg gccgacgccg
     8701 ctcacaatcg gggtacggca ggcggcgatg gtccgtgcca gggactcgtc gttgaagggc
     8761 cagagatcct caatggaacc gccgccccgg gcgatgatga tgacgtcgca gagcccgtcc
     8821 cggtcggcct ggcgcagggc ggccaccagc tcggcggcag ccgtttctcc ctggaccgga
     8881 gaggggtaga tcaggatctc gattcccggg gcacgcccgg cactggtctc gatgatgtcg
     8941 tggaggacgg agcctccggt cgaggtgatg acaccgaccc ggcgtggata ccaggggagg
     9001 ggttttttga gcgattcgga aaagagtccc tcatcggcga gtttccgctt gagttcctcg
     9061 aagcggcggt agaggtctcc ttcgccggct tcgggggtga ccccgctgac cacgatctgg
     9121 acctggcctc gatcgctgta gaatgagacc cggccggcga agaccgcctt cattccgtcc
     9181 ttgagccgca ggccggccgg gagggcccgc cgcactcccg cgaagaggac ggctgagatg
     9241 gaggccttgg cttccttgat ggtgaaatag acgtgccccg agtcgtattc ccggaggttc
     9301 gaaatctcgc ccgagacgag gacggacgaa aatccctcct cgagggtgtt cttgatcagg
     9361 gcgcagagct gacccacgga gaggacgggg gctgcctccc ctggggaatg ctggtgcagt
     9421 ggtgtcacgg ggacaaggta cgaat
//
LOCUS       DAOSHE010000026         9342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6276_length_9342_cov_4.663939, whole genome shotgun sequence.
ACCESSION   DAOSHE010000026 DAOSHE010000000
VERSION     DAOSHE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6276_length_9342_cov_4.663939"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLD82_01465"
     CDS             <1..431
                     /locus_tag="PLD82_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64086.1"
                     /translation="RRFYAKIAGPILDRIDLQVPMMKAREGDLPEPSNFSRWKKALLI
                     ARQRQTERYQNCGRPWNGCVSDDLIIHRIDLKTGLDSLLTEYRKRYAPSPRAVASLLR
                     TARTIADLDDEDAILDRHLLESMQYRKLEEHIKQDQTLWK"
     gene            592..1347
                     /locus_tag="PLD82_01470"
     CDS             592..1347
                     /locus_tag="PLD82_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG64087.1"
                     /translation="MKKSDIAIYVSAILTLVLTTFVVFSTQKVTYENQADEVLKNNNL
                     ATNQFAMASDPDTSSDANSPLFRNRVADGSSDDIKTEQGSEDGKKAADIRDVSDDTAG
                     KKADETDDTRTRATETDTHKKEAKTPVRPDTKAADESFADTTLPRQTTPATKSTGSQN
                     DLFDDEAPTTTVRKPAPSTTRDVLDVPARTPAKKAAAPSIETSSGKKHVVQYGEMLRS
                     IAARNGTTTTKLIQLNNISNPDLIYPGQIIRLP"
     gene            1355..2302
                     /locus_tag="PLD82_01475"
     CDS             1355..2302
                     /locus_tag="PLD82_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64088.1"
                     /translation="MGPSPAAWIGVVALILCLVPGFMAMSDQSATATTGFGEHSLALS
                     NRLVAGIPPDREGVLLALACFRSNSSWMINTIWRSWPELVLREGTNAKAFVESGGMFP
                     GAVLDALLALRGFSGNAASPLSSGVYWLMGQKRYRDALDRIRDSGLATNLRPLAAWLA
                     LKTGENELALEYLRAHVTAQADPPFDEMKLVFNLALRPGAYQEAVYWGNRMLTKAPGL
                     EKNLQWVNNMAFALTEKARSKRGEEAHMTRLNALRYARSALELSRSAPVLHTMAVVCA
                     ALSNYQDAARHLQLALALDTGNTNYRSFLLEMQRKIRGL"
     gene            2304..3194
                     /locus_tag="PLD82_01480"
     CDS             2304..3194
                     /locus_tag="PLD82_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64089.1"
                     /translation="MTQGQVLLLALLVSVPLVSSVPEPAITVMAKGDYQYQSGDLAAA
                     IISYQEALRLDPSLVETHYKLGRIFQSQQAWNLALYHFEELVKKSKTLTYIHLHLDAL
                     FDLSQTHFKIGESIDPGSIRNPHIKKMQDYLQDVIDALKPGGLIDSLGSQYIDISRDY
                     YLARAWYILARIYEDKNQDHKYPEHYINAIRHLDKFRTQQKRAENIVRVAFRESECLY
                     ALYRHHRRIKEFKKAREWEGRARLIQKDILAMAQTLERDPASSWSPDIAVYKAGARNI
                     DLLFAGKVKIDPLFRFFPVK"
     gene            3480..3812
                     /locus_tag="PLD82_01485"
     CDS             3480..3812
                     /locus_tag="PLD82_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64090.1"
                     /translation="MANVTHFGLHNTYYRSSGRAITGHFLTRNSFVTRTKPRIKSASR
                     EALSPASKQKASPLSANKESRRKEANKGTETFKDYLRIYFKSKYEVNVFFDVSFIMAS
                     NPGVIEAS"
     gene            complement(3829..4443)
                     /locus_tag="PLD82_01490"
     CDS             complement(3829..4443)
                     /locus_tag="PLD82_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64091.1"
                     /translation="MSEERKQEGQGSSGKKKNNRPRWFHKKNKNKNGQPQNQQRGQQD
                     QSARSGQDRGRQHQQRRQQQNQQRDGEIQGMPMQMVDGKLRRRDKLVSVFNIQQVMDK
                     SQADSKKEILRLKQEKKSCALCNQEIEDMLSAVGHSEGDGWCHFECVQKELAKVETLA
                     ENEKIVYVGNGDFCIVQERRNRGKMYYFLRKRIHWRESEKSKKI"
     gene            complement(4458..5567)
                     /locus_tag="PLD82_01495"
     CDS             complement(4458..5567)
                     /locus_tag="PLD82_01495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG64092.1"
                     /translation="MSHISKRMSTIDASGIRKVFDLGAKMKDPVNLSIGQPDFDVPDE
                     LKTIAISAIQSGFNRYTQTQGTPELNEAIRAYLKRTAGFEPDSTMVASGVSGALFLLI
                     LATIDPGDEVVFPDPYFVMYKHLVNFVGGVPVCVDTYPDFQLDPEKLAAAITDKTRMI
                     IINSPSNPTGVVYSLETVQAIAELARKRNILLVSDEIYREFCFTKDFPSLATWYPEKT
                     VVLNGLSKSVAMTGWRVGFAAGPADIISEMIKLQQYTFVCAPSFAQKAAVRAFELPNG
                     HLQSYIQRRDQIYHGLVNAGYDVVKPEGAFYIFPKCPADESLFIDAALENKLLIIPGS
                     VFSEKKGWFRISFAADTTVLEKGIEILTRLRKQFA"
     gene            complement(5660..5842)
                     /locus_tag="PLD82_01500"
     CDS             complement(5660..5842)
                     /locus_tag="PLD82_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64093.1"
                     /translation="MMDSNQSKDMICSEPQSPAVRGVDVDRDGRIRDLREKINDTRYL
                     ENALKKIASDLAREFY"
     gene            complement(5878..6255)
                     /locus_tag="PLD82_01505"
     CDS             complement(5878..6255)
                     /locus_tag="PLD82_01505"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG64094.1"
                     /translation="MKKTSGMGRERQIAGQQGEERAVSYLTEVLGYSLLSRNFRTRDG
                     EIDIILMDGETLVFVEVRTTREKVFGPDSINFKKKQKMCKVALKYLENLKRDYKRMRF
                     DAVFVEREPAEGLEHVENIIMLA"
     gene            complement(6230..7498)
                     /locus_tag="PLD82_01510"
     CDS             complement(6230..7498)
                     /locus_tag="PLD82_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG64095.1"
                     /translation="MGEQEKQGLKRIRTKDLADGMMFTSTVYIDKKNIFVSANVPIKK
                     KDIERLLNWGILELETAGEIVPETIPVQKTSPQDSPHRVEAVARMEAELKKLVEEQLR
                     NRKTPLPLKEPTEKQAPGRDLGARVTLAEYHTSWIEDCEKQITSARNNVRLDRGEAGR
                     VVKEILQLAERDSQGLLGQITHRQKGEYLAVHGTNVAVYSAIIGMRMGIEREALTNLV
                     MGCYFLDIGMVRVPDEITQKEGRLEIEELKKIHAHPVYGYQILVQQNGFPIEVGMVAL
                     EHHEKINGVGYPRKLSGRAITLFGRLAAILDTYEAMTGHRTYRDEYISHEAMKNLIAS
                     GQTLFDNDLLATFLREVGVYPVGSRVLLNNNAVGVVIATDPALPMRPELQIVRDEFGD
                     PVLHREVVRLAQERDLVIMQAIDEKDIRNG"
     gene            complement(7491..7769)
                     /locus_tag="PLD82_01515"
     CDS             complement(7491..7769)
                     /locus_tag="PLD82_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOG64096.1"
                     /translation="MPKPENLQAFAMGYQEGDHAPRLLARGRAERAKRMIELAEEAGI
                     PVQEDKDLAEVLNVLGEGEYIPEKLYEAFAVLLAGIYAANERLGKRNG"
     gene            complement(7756..8559)
                     /locus_tag="PLD82_01520"
     CDS             complement(7756..8559)
                     /locus_tag="PLD82_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64097.1"
                     /translation="MRGRVVASGNGMVFEHPSGKIAMERAGQSETGWKNWRVEQGPSG
                     LVLRAVSKAGQASTGPQDPLTALQRMLTILFPGRRAHKGERTSAQREAASTVETLLLA
                     AHPESEQIVRELCKKIRTHRSPLELGNTLYGQAKEGVADEKGVRLHAMDQGDASLLVC
                     HKVFPDNENVPVLLVRTGQGKDAVIWLCTGLELSELGAVFIGLRMDQHRISADLWCHE
                     QHMETLREELGVRCPWPVRIRGTREVPEVLAWSFASIMESLPGGVDAKA"
     gene            complement(8642..9247)
                     /locus_tag="PLD82_01525"
     CDS             complement(8642..9247)
                     /locus_tag="PLD82_01525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG64098.1"
                     /translation="MIYPDDSREEALAARGYRWIAGVDEAGRGCLAGPVVASAVILGE
                     WRHPRINDSKKLTPKMREFLYDEIMAHALAVGVGMVGEEEIDRINILEASRLAMQIAV
                     LQLKPGPDALLLDAIQIEHPAHQVSLVHGDAVSISIAAASIIAKVTRDRLMPALDQAC
                     PGYDFTIHKGYGTARHLEALTRLGPCRFHRKTFTPVRNLLQ"
     gene            complement(9244..>9342)
                     /locus_tag="PLD82_01530"
     CDS             complement(9244..>9342)
                     /locus_tag="PLD82_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG64099.1"
                     /translation="IVRHGQVRRAKLYYLRDRVGKAAQVKEKITRA"
BASE COUNT         2237 a         2608 c         2287 g         2210 t
ORIGIN      
        1 tcagacgatt ctatgcaaaa atcgccggac cgatactgga ccggatcgat ctgcaagttc
       61 cgatgatgaa agcacgggaa ggggacttgc ccgaaccatc aaatttttcc agatggaaaa
      121 aagcacttct cattgccagg caacgacaga cagaacggta tcaaaactgc ggccggccat
      181 ggaatggctg tgtcagtgac gatctcatca tccacaggat tgacctgaaa accggattgg
      241 atagcttgct gaccgaatac cgaaaacggt acgcgccttc accgagagcc gtcgccagtc
      301 tcttgagaac cgcccggacg atagcagatc ttgatgacga agatgcaatc ctcgacagac
      361 atcttcttga atccatgcaa tacagaaaac ttgaggaaca tatcaagcag gaccagactc
      421 tctggaagtg actcttaaag tatctccacg attctcttta catattgcag aataatgaga
      481 tacaacaaaa aaaaatcagg tttttgcacg aaagttcttg caatcccttc cgatttcgtt
      541 tacatattgt gtcggatttc cgatgatggg aaaaaacggg aggggtttgt catgaagaag
      601 tctgatattg caatttatgt ttccgctatc cttacacttg ttctcacgac atttgtcgtc
      661 ttctcgacgc aaaaggtcac gtacgaaaac caggctgatg aagttttgaa aaacaacaat
      721 ctggcgacaa accagtttgc catggcgagc gatcccgaca catcgagtga cgcgaattct
      781 ccattgttcc gcaaccgtgt cgccgatgga tcgagcgatg atatcaaaac cgaacagggc
      841 tcggaagacg gaaaaaaagc tgcggacatc cgtgatgtga gcgacgacac agccggaaaa
      901 aaagctgatg agaccgacga cacaagaact cgggctaccg aaaccgacac ccacaaaaaa
      961 gaggcaaaaa ctccggttcg tcctgacaca aaggcagccg acgagtcctt cgcagataca
     1021 acgcttccca gacagaccac accggcgacc aagtctaccg gttcgcaaaa tgatctcttt
     1081 gacgacgaag ctccgacgac gacggtccgc aagcctgcac cgtcgacaac ccgcgatgtt
     1141 ttggacgtgc ctgcccggac accggccaaa aaggcggcag caccttcgat cgaaacatcg
     1201 agcggtaaaa aacacgtcgt tcagtacggt gaaatgttgc gcagcatagc cgcacgcaac
     1261 gggacgacaa ccaccaagct gatccagctc aacaacatct ccaacccgga cttgatctat
     1321 cccggccaaa tcatcaggct cccctgagac gagaatggga ccgtctcctg cggcgtggat
     1381 cggtgtcgtt gccttgattc tctgccttgt cccgggattc atggccatga gtgaccagtc
     1441 cgcaacggca accaccggat tcggagagca ttctcttgct ctctccaaca ggctggtggc
     1501 cgggatcccg ccggacaggg agggcgtcct gcttgctctg gcttgtttcc ggagcaacag
     1561 cagctggatg atcaacacga tctggagatc ctggcccgaa cttgttctgc gtgagggaac
     1621 caatgccaaa gccttcgtcg agtcgggagg gatgtttccc ggagctgtcc tggatgcctt
     1681 gcttgccctg cggggctttt cggggaacgc agcaagtcca ctgtcaagcg gtgtctactg
     1741 gttgatgggg cagaaacgct accgtgacgc gcttgatcgc atccgggatt caggtcttgc
     1801 gacaaatctg cgcccccttg cggcttggtt ggctctgaaa accggcgaga atgaactggc
     1861 gttggagtat ctccgggctc atgtgactgc acaggccgat cccccgttcg acgagatgaa
     1921 actggtgttc aatctcgccc tcaggccggg agcatatcag gaagccgtct actggggaaa
     1981 ccggatgctc acaaaggcgc ccgggctgga gaagaatctt caatgggtga acaatatggc
     2041 gtttgcactg acggagaaag cccggtcgaa gcgtggagag gaagcccata tgacaaggct
     2101 caacgccctg cgatatgcaa gatcggccct ggagttgtct cgttctgcac ctgtccttca
     2161 tacgatggct gtggtttgcg cggctctctc gaactatcag gatgcggcac gacatctgca
     2221 attggcactt gccctcgaca cggggaacac gaattatcgc agtttcctgt tggagatgca
     2281 gagaaaaatc agggggttat gaaatgactc agggacaggt cctgcttctg gctctcctgg
     2341 tatcggtacc actcgtatcg tcggttcccg agcctgccat taccgtcatg gcaaaaggtg
     2401 actaccagta ccagtccgga gatcttgcgg cagccatcat ctcgtatcag gaagcgctcc
     2461 gtcttgaccc gagtcttgtc gaaacccatt ataaactcgg caggatcttt caatcacagc
     2521 aagcctggaa tctcgcattg taccatttcg aagagctcgt caagaaatcc aaaacgctga
     2581 catacatcca tctccatctt gatgccttgt ttgatctctc ccagacccat ttcaagatcg
     2641 gagaaagcat tgatccgggt tcaatcagga atccccatat caagaaaatg caggattatc
     2701 tgcaggatgt cattgatgca ctgaaacctg gtggtttaat tgacagtctt ggttcacagt
     2761 acatcgatat ctccagggat tactatcttg cccgggcctg gtacatcctg gcgcggatct
     2821 acgaagacaa gaatcaagac cacaaatacc ctgaacatta catcaatgcg atccgccatc
     2881 tcgataaatt ccgcactcag caaaaaaggg cggagaacat cgtgcgtgtc gcttttcgtg
     2941 aatcggagtg cctgtatgca ctctatcgtc accaccgacg tatcaaggaa ttcaaaaagg
     3001 cacgggagtg ggagggaagg gcacgtctga tacaaaagga catcctcgcg atggctcaaa
     3061 cccttgagcg agatccggct tcgtcgtgga gtcctgatat cgccgtgtac aaggctggag
     3121 ccagaaacat tgatctcctg tttgccggca aggtgaaaat cgatcctctg ttccgcttct
     3181 ttcctgtaaa ataagcactc aaatacgagc gtcatcagcg cggtgtcctt atggtatcgt
     3241 ttcttcctgt cgcaggatgt gagcagcatg gaggacgcca aaatcctgtc gcgcgttcca
     3301 tcccttggca gatcccggac tgaggctcca gctacaaacc atcatacgga gtcttccaca
     3361 cgggaatcgc atcgcctttc ctactacact acacaaattg aaccgacatc gggggagcga
     3421 caaataccca ttcattgatt ggagacaaca aaacaatacg gttgaggcca acccgactct
     3481 tggcaaatgt cacgcatttt ggtttacata atacatatta caggtcttct ggtagagcaa
     3541 taacaggcca ttttcttaca agaaactcct tcgtaacaag aaccaagcca cggataaagt
     3601 cggcaagcag agaagcactt tctcccgcca gcaagcagaa agccagcccc ttgtcagcaa
     3661 acaaagaatc aagaagaaaa gaagcaaaca aaggaaccga gacttttaaa gactacttga
     3721 gaatttactt taaaagcaaa tatgaagtga atgtgttttt tgatgtttct ttcatcatgg
     3781 cttcaaatcc tggagtaatc gaagcaagtt gaatttctgg tggcagaatc agattttctt
     3841 gctcttttcg gactcacgcc aatgaatccg ttttcgaaga aaatagtaca tcttgccgcg
     3901 attccgtctc tcctgaacaa tgcagaaatc gccatttccc acatagacaa ttttctcgtt
     3961 ttcagccaag gtttcaactt tcgccagttc tttctggacg cattcaaaat gacaccagcc
     4021 atccccttcg gaatggccga cagccgagag catgtcttca atttcctgat tacaaagagc
     4081 gcaggatttt ttttcctgct tcaatctgag aatttctttt ttggagtcgg cctggctctt
     4141 atccatgact tgctgaatat tgaaaacgga gacaagcttg tcccgacgtc tcagttttcc
     4201 atcaaccatc tgcatcggca tgccctgaat ctcgccatcg cgctgttgat tctgctgttg
     4261 ccgtcgctgc tgatgctgtc ttccacgatc ctgaccggac cgggcagatt ggtcctgttg
     4321 tccccgttgc tgattctggg gctgtccatt tttgttttta ttttttttat ggaaccatct
     4381 cggacgattg ttcttttttt ttccggagct cccctgcccc tcttgcttcc gctcttcaga
     4441 catgctgtat ctccttgtca ggcaaattgc ttccgcaacc gtgtgaggat ctcgatccct
     4501 ttttcaagga ccgttgtgtc ggccgcaaac gaaatcctga accaaccttt tttttcggag
     4561 aatacggatc ccggaatgat caggagcttg ttttcgagag cggcatcgat aaagaggctc
     4621 tcgtctgccg ggcacttggg gaagatatag aacgcccctt ccggtttgac aacatcgtat
     4681 ccggcattta ccagaccatg atatatctgg tcccgccgtt gaatgtatga ctggaggtgg
     4741 ccattcggca gttcaaaggc ccgtaccgca gccttctgcg caaaggatgg cgcacacaca
     4801 aaggtgtatt gctgaagctt gatcatctcg ctgatgatgt cggccggccc ggcagcaaaa
     4861 ccgacccgcc aaccggtcat cgcaaccgat tttgacaatc cgttcaagac aaccgtcttt
     4921 tcgggatacc atgtggcaag actgggaaaa tctttggtga aacagaactc ccgatagatc
     4981 tcgtcagaaa caaggaggat attccgtttc cgcgccaact cggcgatggc ttgaaccgtt
     5041 tcaagagaat agaccacgcc tgtcggattt gacggactgt tgatgatgat catcctggtt
     5101 ttgtctgtta tggctgcagc aagcttttcg ggatcaagct gaaaatccgg ataggtatca
     5161 acgcagacag gcacaccacc gacaaaattg acaagatgct tgtacatcac aaaatacgga
     5221 tcagggaaaa caacttcatc acccggatcg atggttgcca gaatcaacag gaagagcgcc
     5281 ccggaaacac cggacgcgac catagtgcta tccggctcga aaccggctgt ccgtttgaga
     5341 taggcacgga ttgcttcgtt caactccggg gtaccctgag tctgtgtata ccggttgaaa
     5401 ccggattgaa tggcactgat ggcaattgtc ttgagttcgt cgggaacgtc aaaatcaggc
     5461 tgtccgatac tgagattgac cggatccttc atcttggcac ccaggtcgaa gaccttcctg
     5521 atcccggatg catctatggt ggacattcgt ttcgagatat ggctcatggc cgacccgcct
     5581 tggatttcat gtacccatga aacataggca tggaatcagc ctgcttcaag aggatttctt
     5641 cacccctggc tgccctgtat caatagaact cacgggccag atctgaagca atttttttca
     5701 aagcgttttc aaggtaccgt gtatcattga tcttctcacg cagatcccga atgcggccat
     5761 ccctgtccac atcaacgcct ctaaccgcag gtgactgcgg ttcgctacag atcatgtcct
     5821 tgctctggtt actgtccatc atttgtcaat cctttgacct ggtggccgac atccttgtca
     5881 agccagcatg ataatattct ctacatgctc aagaccctca gcaggctccc tttccacgaa
     5941 aacggcgtca aaccgcattc gcttgtagtc cctcttcaga ttttcgagat atttgagcgc
     6001 aactttacac attttttgct tttttttgaa atttatcgaa tcgggcccaa acaccttttc
     6061 ccgcgtggtc ctgacctcca caaaaacaag tgtctcacca tccatcagaa tgatgtcaat
     6121 ctcgccatct cgtgtccgga aattcctgga caagagggaa tacccaagaa cctctgtcaa
     6181 atagctgacg gcacgttctt caccctgttg accggcaatc tggcgttccc tacccattcc
     6241 ggatgtcttt ttcatcaatg gcctgcatga tcaccaggtc acgttcctgt gccagtctga
     6301 cgacctcccg atggagaacg ggatctccga attcatcccg gacaatctgc agttcaggcc
     6361 gcatcggaag ggcgggatcg gttgcgatga ccaccccgac cgcattgttg ttgagtagga
     6421 cccgcgaacc aacgggatag acaccgacct ctctgagaaa cgttgcaaga aggtcgttgt
     6481 caaacagggt ttggccactg gcaatcagat tcttcatggc ttcgtgggag atatactcat
     6541 cccggtaggt cctgtgtccg gtcatcgcct cataggtatc aagaatggcg gccagacggc
     6601 caaagagtgt aatcgctctg ccggagagtt tccgcggata cccgaccccg ttgatttttt
     6661 cgtgatgctc gagtgccacc atgccgactt cgatcggaaa tccgttttgc tggacaagga
     6721 tctggtaccc atagaccggg tgcgcatgga tctttttcag ttcttcgatc tcgagccggc
     6781 cctctttttg tgttatctcg tccgggacgc gaaccatccc gatatcgagg aaatagcacc
     6841 ccatgacaag attggtgagg gcctcgcgct caattcccat gcgcatcccg ataatggcac
     6901 tgtacacggc cacattggtt ccatgaaccg cgagatattc ccctttttgc ctgtgtgtga
     6961 tctgtcccaa aagcccctga ctgtcacgct ccgcaagttg caggatctcc ttgacaaccc
     7021 gtcctgcctc gccccggtca agccggacat tatttcgggc tgacgtaatc tgtttttcac
     7081 aatcctctat ccaggaggta tgatactcgg cgagtgtgac ccgggccccc aggtccctcc
     7141 cgggagcctg cttctcggtc ggctctttga gcgggagcgg ggttttgcga ttccgaagct
     7201 gctcttcaac aagctttttc agttccgctt ccatccgggc gacggcctcc acccggtgcg
     7261 gggaatcctg gggactggtt ttttgaaccg gaatggtctc cgggacgatc tcacccgccg
     7321 tctccagctc gagaatcccc caattcagaa gacgctcaat atccttcttt ttgatcggaa
     7381 cattggccga aacaaagatg ttttttttat cgatatagac tgtgcttgtg aacatcatcc
     7441 cgtcagccag atctttcgtg cggatccttt tcagtccctg tttttcttgt tcacccattc
     7501 cttttcccca atcgctcatt tgctgcatag atccccgcca ggagcaccgc gaacgcctca
     7561 tacaatttct cgggaatgta ctcgccctcc ccaagcacgt tcaagacttc cgccaaatcc
     7621 ttgtcctcct gaaccggaat accggcttcc tcggccaact cgatcatgcg cttcgcacgt
     7681 tcagccctgc cccgggcgag gagtctgggc gcatgatccc cttcctgata ccccatcgcg
     7741 aatgcctgca agttctcagg ctttggcatc gacgcccccc ggaagactct ccatgatact
     7801 cgcaaaggac caggcaagga cttcgggaac ctcccgagtc ccccgtatcc gcaccggcca
     7861 tggacaacgt acacccagtt cctcgcgcaa cgtctccatg tgctgctcat gacaccagag
     7921 gtcagcactg atccggtgct gatccatgcg aagaccgatg aaaactgccc ccaattcaga
     7981 caactccaag ccggtacaga gccagatcac agcatccttt ccctggccgg tccggacgag
     8041 caggaccggg acattctcat tatcgggaaa gaccttgtgg cagacaagca gactcgcatc
     8101 tccctgatcc atggcgtgca gtctcacgcc cttctcgtcc gccacgcctt ctttagcctg
     8161 cccgtagagg gtattcccga gttcaagcgg actccggtgc gtccggatct tcttgcagag
     8221 ttcacgaacg atctgctccg attcggggtg cgctgccagg agaagcgttt caacggtgga
     8281 tgccgcttcc ctctgtgcag aagtccgttc ccctttatgt gcccttcggc cgggaaacag
     8341 gatcgtcagc atccgctgga gtgccgtaag cggatcctgc ggccctgtgg aggcctgtcc
     8401 agccttggag acggcacgga ggacaagtcc ggacggtccc tgctccacac gccagttctt
     8461 ccatcctgtc tctgactgtc ctgcgcgctc cattgcgatt ttaccggagg gatgctcaaa
     8521 aaccatcccg ttgcccgaag ccacaacacg tccccgcaca agcattccag gcctgaggac
     8581 tgttccccag acagctgttg aagactgagg ctgaagatgt atgacaggaa gcatgacggc
     8641 tttattgtaa gagattccgg acaggtgtaa aggtttttcg atggaaacgg catggaccaa
     8701 gacgtgtcaa ggcctcaagg tggcgggccg ttccatagcc tttgtggatg gtgaaatcat
     8761 atcccgggca ggcttgatcc agagccggca tcagcctgtc cctcgttacc ttggcaatga
     8821 tggatgcggc ggctatggag atcgagacgg catcaccatg gacaagactg acctgatggg
     8881 cgggatgctc gatctggatg gcatcaagga gcaaagcatc cgggccgggt ttcaattgga
     8941 gaacggctat ctgcatggca agccgactcg cctcgagaat attgatgcgg tcgatttcct
     9001 cttcgccaac catgcccacg ccgactgcga gagcatgagc catgatttca tcatacaaaa
     9061 actcacgcat ctttggcgtg agttttttgg aatcattgat gcggggatgg cgccattccc
     9121 ccagaatcac ggccgacgca acaactggac cggccaagca accccgtccg gcctcgtcga
     9181 caccagcaat ccaacgatat ccccgggcag ccagggcttc ctcacgggaa tcatccggat
     9241 agatcaagcg cgtgtgatct tttccttgac ctgggcagcc ttgccgaccc ggtcgcggag
     9301 ataatacaac ttggccctgc gaacctggcc atgccgcacg at
//
LOCUS       DAOSHE010000027         9269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6339_length_9269_cov_4.072064, whole genome shotgun sequence.
ACCESSION   DAOSHE010000027 DAOSHE010000000
VERSION     DAOSHE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6339_length_9269_cov_4.072064"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..672)
                     /locus_tag="PLD82_01535"
     CDS             complement(373..672)
                     /locus_tag="PLD82_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG64100.1"
                     /translation="MATWEEISEARGVLGVGEDATLAEIRRIFRDRIKAAHPDAGGTR
                     GESARVTAAWKILREYCEGYRFRFTEDEVRRNESPEERMKRMFRQDTQWGPGQRH"
     gene            791..1576
                     /locus_tag="PLD82_01540"
     CDS             791..1576
                     /locus_tag="PLD82_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOG64101.1"
                     /translation="MERLREQLAWLHEHHGLVGIKGGTEVEAQTFDEIWLMRRLSDKI
                     VPLTVKIGGPEARNDIEFMLATGVDCILAPMVESPYSMRNFVSTMATLDRTGQAELAV
                     NIETITAWRNLDDIFDSPAFDSIAQITVGRSDLSGSMGLEVDDDEVSRVTRDIISAAR
                     LRGKRTSVGGQVTTENARLVQDTLGAHCINTRHMLVSLSSGDASANIAAALLWEREYY
                     NFCKKHFPTRSSFYRSRIASIEERIAAGEADCPLITGLALTGT"
     assembly_gap    1708..1807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1808..2841)
                     /locus_tag="PLD82_01545"
     CDS             complement(<1808..2841)
                     /locus_tag="PLD82_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG64102.1"
                     /translation="MQEDTRGNLRRRAEEILESRKQDHDGFSDDRDAVIHELLVNQIE
                     LEMQNDELRRIQIELDTAQQRYRQLYEFSPVALVRLTDGLLVEDCNIMTSEMLRCDKA
                     GLERKPLSRWVHEEDQDAYFLLMRKVLASKTPSMQEVRFRIGSDILWCVMRVGLLESG
                     NNRKQYLEVAILDVTDTHRAEESLRITEENLGATWFGYLGRWDWEYASGRVVSNRLKT
                     EVLGYGPGEIAPHVDAFTSLIHPDDFDRTMANMRDHLMGKTSVYEVEYRIRCKDGGWK
                     WFYDRGLVVERDAVGKPRRIAGIVFDIDEKRRQMVELERVAAELRRSNEDKDRLLSII
                     AHDMRSPVTAA"
     gene            3000..3260
                     /locus_tag="PLD82_01550"
     CDS             3000..3260
                     /locus_tag="PLD82_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64103.1"
                     /translation="MERTILLVLVSQRTEHAGQVQRILTENGRIIRTRLGLHDAGDTA
                     CANSGLLVLELTGNASDHVKLNEELSQLPGVTTRLVSLPVTD"
     gene            3306..4034
                     /locus_tag="PLD82_01555"
     CDS             3306..4034
                     /locus_tag="PLD82_01555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG64104.1"
                     /translation="MPVSVCPYFPHQPTGSAPSVFLQGTDLSPEECDELLDQGWRHFG
                     RQYYRNDCPGCQRCMPLRIPVMHLELSHSQRRLLRKSTDLESRFFSPEEFSVRHFFEA
                     LEIHNAFIQTRHGHRAVTPGEFHASFFSSPAPLVIHGLFDPNGILIGCGWLDAGRRSL
                     SSIYYSWHPDSAKHSPGTLSMLREALYSRETGRTYYHLGYWIEGHRSMAYKAGLHPSE
                     ILLFCNADWISLPADSLRLRHSPT"
     gene            complement(4009..5688)
                     /locus_tag="PLD82_01560"
     CDS             complement(4009..5688)
                     /locus_tag="PLD82_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG64105.1"
                     /translation="MATRKQSDRKAKTTEGSILFVAAECRGMAKIGGLGDVVRDLAAT
                     FHGMGRAVSVVMPYYGVLECSSEEIAVFDVPFAGKSWKAGLHRSVLPDGVTVWLISSE
                     EWFGGPYRTVYVDSSQLGHGPFEDDATRFAFFSAAVLERLEAGPEFSGVEVLHCHDWH
                     TGPLLTLLEHEQRHAELRKRLKTVFTIHNLDYQGSRPWQGMDGGRPGSFGSWFPLVAG
                     TLLNSGKAKEYEDPHNPACYDPMRAAVRLADMVTTVSPTYAREITMPDEPESNFVGGR
                     GLEADLGTLATLGRLPGILNGIDYAQHDPKKLPVPYDVDMKDWRQRRIQGREAFVDGI
                     PEWVRELAGRHGERFANMEHVLEAVRHFEPLLWKNRTLFVAVTRTVAQKFGILLTGMK
                     DGRTLLEHILDRPLALIVLGSGDLEHWLDALMQRPNALFLNLFDAGAATRLYGAGDVF
                     LMPSDFEPCGISQMISMRYGCLPLVHDIGGLHDTVIHGETGFTYGGGSLQAQQKTLLS
                     LVDDVVWLRENRAQRWAGMQEKAMRARFAWTTQAERYLEIYSSIRSGNAGA"
     gene            complement(5805..7493)
                     /locus_tag="PLD82_01565"
     CDS             complement(5805..7493)
                     /locus_tag="PLD82_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64106.1"
                     /translation="MSADRRPLSPAGERSLIERLGREGYFLREARLSETASLLAVLAW
                     GLALVFLSGSPVNGMQPLVVNLGPVWWRYSLFGDLAILLAVLRYCNSIEGQGQRLALG
                     LLGLLAVAHGLTSWHDPWGAVFSNTLIEWLLLLSALVTILPFLQKIGTTIDWCMAGAF
                     AVGAILVALSSFGVHALSLAGLAARRLVVKLVAGAGAGSLLPVYRWGLALSAISLIFL
                     ILRLRRRRLALDPGLGALRKRALLEHEGKRKWHHLWVVLVLLCIPSGGGLRARSVSLD
                     RMLTNDGISREDLARAFAGLGRSGNRYRFEAVTNLVLYEEPPAVRGADPLFQETIVQE
                     IRNALLTNGTAWARARLAGQEALPQWMTALLSGEAALKAGERDAAARAFVSCLVLQPG
                     EHRAWDGLAAAGRDPLRFVFPVRARAVSRFPDEVVIQYDAGLMAIQERYLWMSYALAR
                     ALFRFEGLWKDGTGTGDWYEESFEEHLFAWRVVAAVWRGYDKDPLKGAAPFFTRLASL
                     DRDGLLPGLVWFELWHPVSPKVAEAVRIRFRLAIERYCERILLRKYGESGPDGS"
     gene            complement(7537..8811)
                     /gene="serS"
                     /locus_tag="PLD82_01570"
     CDS             complement(7537..8811)
                     /gene="serS"
                     /locus_tag="PLD82_01570"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG64107.1"
                     /translation="MLDIRLVRENPQAVKAALARVGDTTSIDALLELDAKRRTLVGEI
                     ESMRSERNAVSKAIPSIKDPAEKQIKISEMRALGDRLALLEKEGAEIDSLFETVMLSI
                     PNMPSDETPDGADDTANRVTGEYGSKPEFGFQPKTHWEIGESTGLIDFERGTKISGTR
                     FYILKGELARLQRALISFMLDVHTREHGFVEVYPPFMVKEHCLYGTGQLPKFGENLYH
                     DAEEDFWFVPTAEVPVTNMYRDEILDGAGLPLVHVAYTACFRREKMSAGKDVRGIKRG
                     HQFDKVEMVAFVRPEESPAMLIKLMESAEHILAKLGLPWRRLQICAGDLSFTAAAKYD
                     LEVWAAGCGEWLEVSSISNFRDFQARRAGIRFRRNAGDKPEFVHTLNGSGLALPRIMI
                     AILENYQMEDGRVCVPEVLLPWMGGTTILGQG"
BASE COUNT         2052 a         2952 c         2368 g         1797 t
ORIGIN      
        1 cgtatatcaa ggaaaactat tacacgatct tttcggaaaa agaaaaacgg gaggaagccc
       61 gggatctgct ggagactgat gcccttctca aggagctgac ggtataaaaa agtcaccata
      121 gcgctctcac cttgggcacg tccggcacca tggtgcgctt tccgacccgt ctatcaggcc
      181 attattggcc ctccattctg cgcatcatgg tgacatttat atgggcaacc cgccctacta
      241 tggtgatgtc gccagccaga cccgcgccat ggtgcatttt tcagcattcc ggcccccgtt
      301 ttccgacatc atggtaccgt ttttcgcgat tccgggaaaa tgccaccaga gtaggtgtcc
      361 tgaagattcc gatcagtgcc gctgtcccgg cccccattgg gtatcctgac gaaacatccg
      421 cttcatccgt tcctcggggg actcattgcg gcgcacctca tcctcggtaa agcggaatcg
      481 gtagccctca cagtactccc tgagtatttt ccacgccgcg gtcacccggg cactctcgcc
      541 acgagtccct ccagcgtccg gatgcgccgc cttgatccgg tcccggaaaa tacgccggat
      601 ctcggccaat gttgcatcct cgccaacccc caggactccc cgggcctcgc tgatttcttc
      661 ccaagtggcc atgatgctcc tccgcaaacc atccaaggtt cctcaatccc caacagcagt
      721 aatatgctca actcgcgccg tgaaaaggcc gatattttgt gtatatatcc tgtcactcgg
      781 ggagggaccc atggaacgac tcagagaaca gcttgcctgg ctgcacgagc accatggact
      841 tgtcggcatc aagggcggta ccgaggttga agcacagacc tttgacgaga tctggctgat
      901 gcgccggctc tccgacaaga tcgtccccct gaccgtaaaa atcggcggac ccgaggcccg
      961 caacgatatc gagttcatgc tggctaccgg ggtcgattgc attctggccc cgatggtcga
     1021 atcaccatac agcatgcgga attttgtttc cacaatggca acactggatc gaacaggaca
     1081 ggcggaactt gccgtcaata tcgaaaccat caccgcatgg cgcaacctcg acgatatttt
     1141 tgacagcccg gcatttgact cgatcgcgca gatcacggtt ggtcgctcag atctctccgg
     1201 ctcgatggga ctcgaggttg acgatgacga ggtcagtcgg gtcacccggg acatcatcag
     1261 cgcagccagg ctccgcggaa aacgaacctc ggtcggcggg caagtcacaa ctgaaaacgc
     1321 ccgtctcgtc caggacaccc tcggcgcaca ctgcatcaac acacgacaca tgctggtcag
     1381 tctttccagc ggggatgcct cggccaatat cgctgccgcc ctgctctggg agcgtgagta
     1441 ctacaacttc tgtaaaaagc acttccccac ccgatcttcc ttctatcgtt cgcggatcgc
     1501 ttcaattgaa gaacggattg cagcgggtga agcggactgc cccctgatca ccggcttggc
     1561 attgacggga acctgattcc ggcaaattca aggcaggatc acgataaatg aacttcccgc
     1621 accggaagta ctctcgacac ggatccgccc tccggcaatc tgtacaaaat catggcagag
     1681 cgccaagcca agcccgctcc cggcctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnngcg gccgtcaccg ggctgcgcat atcgtgggca atgatggaca aaagccgatc
     1861 cttgtcctca ttggagcgtc gcagttctgc agcaaccctc tcaagctcta ccatctgcct
     1921 gcgcttctcg tcaatgtcaa acacaattcc cgcaatccgt cttggcttgc cgactgcatc
     1981 acgctcgacc acaagccccc tgtcgtaaaa ccatttccac ccgccatcct tgcagcggat
     2041 ccggtattcg acctcataca ccgaagtctt gcccatcaga tgatcccgca tatttgccat
     2101 ggtccggtca aagtcatcgg gatggatcag actggtgaaa gcgtccacat gaggtgctat
     2161 ctctcctggt ccatacccaa gcacctctgt tttcaacctg tttgacacaa cccgaccact
     2221 ggcatactcc caatcccaac ggccaagata cccgaaccag gttgcgccga ggttctcttc
     2281 ggttatccgc aaggactcct cggccctgtg tgtatcggta acatcgagaa tcgccacctc
     2341 gagatactgc ttcctgttat taccgctctc cagcaagcct acccgcatca cacaccagag
     2401 aatatcgctg ccaatccgga acctgacctc ctgcatggac ggggtcttgc tcgcaagaac
     2461 ttttctcatc agcaaaaaat aggcatcctg gtcttcttcg tgaacccagc gtgacaacgg
     2521 cttgcgctcg agcccggctt tgtcacatcg caacatctcg cttgtcatga tgttgcaatc
     2581 ttcgacaagc aacccgtcag tcagccgcac cagtgcaacc ggagagaatt catacaactg
     2641 cctgtatcgt tgttgtgccg tatccagttc gatctgtatc ctgcgcaact cgtcgttctg
     2701 catttccagt tcgatctggt tgaccaagag ttcatgaatg accgcatccc ggtcatccga
     2761 aaagccatcg tgatcctgct tcctgctctc aaggatttct tcggctcttc tgcgcaaatt
     2821 gccccgggta tcttcctgca tgatagccct ccaatacaag cgcccggtta cccaaatagt
     2881 actcctgaat cgccacttcg taaagtgcgg aaaatactat cccgacacag aacagggctt
     2941 gacgaatgaa cactccgacc ggatactcaa tagcagattc gacaacagga gatccatgta
     3001 tggaacgcac cattctactt gttctagttt cacagcgaac cgagcatgcc ggccaggtcc
     3061 aacggatcct caccgagaat gggcgcatca tccgcacccg tctcggcctg cacgatgccg
     3121 gagacacagc ctgcgccaac agcggcttgc tcgtcctcga actgaccgga aacgcctcgg
     3181 atcacgtcaa gctcaatgaa gaactctcac aactccccgg ggtcaccacc cgtctcgtat
     3241 ccctgccagt cactgactaa ggcatggtcg agaagcccct ccaggaccgg tacgggtcca
     3301 tccgcatgcc tgtatctgtc tgtccatatt ttccgcatca gccaacgggt tctgcccctt
     3361 ccgtttttct ccaagggacg gatctctcgc cggaagaatg cgacgaactc cttgaccaag
     3421 gctggcgtca ttttggcaga cagtattatc gcaacgattg tccgggctgt caacgctgca
     3481 tgccgctgcg catccccgtc atgcatctcg agctctccca ctcccagcgc cgtctcctcc
     3541 ggaaatcaac cgaccttgaa tcacgttttt tcagtccgga agaattttcc gtacggcatt
     3601 tctttgaagc tctcgagatc cacaatgcgt tcatccagac cagacatggt catcgcgctg
     3661 tcactccggg cgaattccat gccagcttct tttccagccc ggcgccactg gtcatccatg
     3721 gactctttga tccgaacgga atcctgatcg gctgcggctg gctggatgca ggacgccgct
     3781 cgctttcatc catctactat tcctggcatc ctgattcggc aaaacattca ccgggaacac
     3841 tctccatgct cagagaggca ctctattcgc gtgaaaccgg ccgtacgtat tatcatctcg
     3901 gctattggat cgaggggcat cgttccatgg cctacaaagc aggcctgcat ccctccgaaa
     3961 tcctgttgtt ctgtaatgcc gactggattt ctctcccggc tgacagcctc aggctccggc
     4021 attccccgac ctgatggacg agtagatctc aagataccgc tcggcctgtg ttgtccacgc
     4081 aaaacgcgcc cgcatggcct tctcctgcat accggcccag cgctgcgccc tgttttccct
     4141 gagccagacc acatcatcca caagcgagag caaggttttt tgctgggctt gaagactccc
     4201 gcctccatag gtaaaacccg tctcgccgtg aatgaccgtg tcgtgcaatc caccaatatc
     4261 atgcaccagg gggagacacc cgtagcgcat gctgatcatc tggctgatcc cgcaaggctc
     4321 aaaatccgag ggcatcaaaa aaacatcacc ggctccgtag agccgcgttg ccgcacccgc
     4381 atcaaagaga ttcagaaaga gggcattcgg ccgctgcatc aaagcatcga gccaatgctc
     4441 caggtcaccc gatccgagaa cgatcaacgc cagagggcgg tcgaggatgt gctccaagag
     4501 ggttcgtccg tccttcatcc cggtcaagag gatcccgaat ttctgggcga ctgtccgggt
     4561 cacagcgaca aacagggtcc ggtttttcca gaggagcggt tcaaaatgcc gaaccgcttc
     4621 aagcacatgc tccatgttgg caaaccgctc gccatgccgc ccggccagtt cgcggaccca
     4681 ttccggaatt ccgtccacaa acgcctcccg gccctggatc cggcgctggc gccaatcctt
     4741 catatcgaca tcatagggaa caggaagctt ctttggatcg tgctgggcat agtcaattcc
     4801 gttcagtatc ccgggaagac gcccgagtgt tgccagtgtc cccaggtcgg cttccagacc
     4861 gcgccctccc acaaaattgc tctcgggttc atcgggcatg gtgatctccc gggcataggt
     4921 cggggaaacg gtcgtcacca tgtccgccag acggacagca gcccgcatcg gatcgtaaca
     4981 ggccgggttg tgcggatcct cgtattcctt ggccttgcca ctgttgagaa gcgtccctgc
     5041 gacaagcgga aaccagcttc caaaacttcc cggccggccg ccatccatac cctgccaggg
     5101 tcttgaaccc tgataatcca ggttatggat ggtgaaaacg gtcttgagac gcttccgcaa
     5161 ttccgcatgc cgctgttcat gctcaagaag ggtcaagagc ggtccggtgt gccagtcatg
     5221 acaatgcaaa acctcaacac cggaaaactc ggggccggct tccagtcgct ccagcacggc
     5281 cgccgagaaa aaggcgaagc gggtggcatc atcctcaaag ggaccatgcc cgagttgact
     5341 gctgtcgaca tagacggttc ggtacgggcc gccgaaccac tcctcgctcg agatcagcca
     5401 gaccgtcacg ccatcgggca gaaccgaacg atgcaacccg gctttccagc tttttccggc
     5461 aaacggcaca tcaaaaacgg cgatctcctc ggatgagcac tcaaggacac catagtacgg
     5521 catcacgacg gagacggcgc gccccattcc atgaaaggtt gcggccagat cccggacgac
     5581 atcacccaga ccaccgatct tggccatccc ccggcactcg gccgcaacaa agaggatgga
     5641 gccttccgtt gtctttgcct tgcggtcact ctgcttgcgc gtcgccattt actccacctg
     5701 ccctctctgg atactccagt ctatcccgag tgggcattcc tgtcaacgac acagacagac
     5761 atccataaac aatgcaaaca aaccgatatt ggaattctgt cttgtcagga accatccggt
     5821 ccggactccc cgtacttccg gaggagaatc cgctcgcaat accgttcaat cgccagccgg
     5881 aaccggatgc ggacagcctc ggccactttg ggcgaaaccg ggtgccagag ctcgaaccag
     5941 acgagcccgg gaagaagtcc gtcacgatcg agagaagcca gtctggtgaa aaacggagcc
     6001 gcgcccttga ggggatcctt gtcatatccg cgccaaaccg ccgccacaac ccgccaggca
     6061 aagagatgtt cctcaaaact ctcctcatac cagtcgcccg ttccggtccc gtccttccag
     6121 agaccttcaa aacggaacag ggcccgggcc agggcatagc tcatccagag ataccgttcc
     6181 tggatcgcca tcaggcctgc atcgtactgg atgacaacct catcggggaa tctggaaacg
     6241 gcccgggccc ggactggaaa cacaaaccgg agcgggtcac gaccggcagc ggccaggcca
     6301 tcccaggcac gatgttcacc cggttgcagg accagacacg agacgaaggc ccgggccgca
     6361 gcatcccgtt ccccggcctt gagggctgcc tcccccgata ggagcgccgt catccattgc
     6421 ggaagcgcct cctggcccgc gagtctggcc cgggcccagg ccgtcccgtt ggtgaggagc
     6481 gcattccgga tttcttgaac gattgtttcc tggaacagag gatccgcccc ccggactgca
     6541 ggtggttcct catacagaac aagattcgtc accgcctcaa accggtagcg attgccggat
     6601 cgccccagac cggcgaaggc ccgggccaag tcctcccggc tgatgccatc attcgtcagc
     6661 atgcggtcca gcgaaaccga cctggcgcgc aagccgcccc ctgaaggaat gcagagcagg
     6721 acaaggacga cccagagatg atgccatttc ctttttccct cgtgctccag caaggcacgc
     6781 tttcgcagcg cgcccaatcc cggatcaagg gcgagccgcc gccggcgcag acggagaatc
     6841 aggaagatga gagagatcgc ggacagtgcc aggccccagc ggtagaccgg caagaggctg
     6901 ccggccccgg caccggcgac caacttgaca acgaggcgcc gggcagccag cccggccagg
     6961 gagagcgcat gcaccccgaa ggacgaaagc gcaaccagga tggcgccgac cgcaaacgca
     7021 cccgccatgc accagtcgat ggttgtcccg attttctgca ggaacggaag gatcgtgaca
     7081 agagcagaca ggagcaggag ccattcgatc agggtattcg agaaaacggc accccaagga
     7141 tcgtgccagc ttgtcaaccc atgcgcaacc gccaggagac caagcaggcc gagggcaagc
     7201 cgctgaccct gcccctcgat cgaattgcag tagcggagaa cagccaggag aatggccaga
     7261 tccccgaaca aggagtaacg ccaccagacc gggcccaggt tcacaacgag gggctgcatc
     7321 ccgttgacgg gagaaccact gagaaacacg agggccaagc cccaggcaag gacagcaaga
     7381 agactggcgg tttcggagag cctggcttcg cgcagaaaat acccttcccg cccgagccgt
     7441 tcgatcagcg accgttcgcc agccggggaa agtggccgtc tatccgcaga catggccctg
     7501 cgccctcact ctgaagcccc ggtcacgcac gcacgctcac ccctgtccga gaatcgttgt
     7561 gccacccatc caggggagga gaacctccgg cacacagacc cggccatctt ccatctggta
     7621 gttttccaga atggcgatca tgatccgggg aagtgccagc ccggagccgt tgagcgtgtg
     7681 cacgaattcg ggcttgtctc ccgcattccg tctgaaacgg atccccgcac gacgggcctg
     7741 gaaatcccgg aaattgctga tggacgagac ttcgagccac tccccacaac cggccgccca
     7801 gacttcgaga tcgtacttgg cagcagcggt gaacgaaaga tcgcccgcgc agatctgcag
     7861 tcggcgccag ggcagaccca gcttggccag aatatgttcg gcactctcca tgagcttgat
     7921 cagcatggcc ggggattcct cgggccggac aaaggccacc atctcgacct tgtcaaactg
     7981 gtgcccgcgc ttgatcccgc gcacatcctt gccggccgac atcttttccc gcctgaaaca
     8041 ggccgtatag gctacatgga ccaagggcag tccggccccg tcgagaatct cgtcccggta
     8101 catattggtc accggaacct cggcggtggg aacaaaccag aaatcctcct ccgcgtcgtg
     8161 gtagaggttc tccccgaact tgggaagctg gccggtcccg tagagacaat gctccttgac
     8221 catgaacggc ggatagactt ccacaaagcc atgctcgcgg gtgtggacat ccagcatgaa
     8281 actgatcagc gccctctgca accgggcgag ttcacccttg aggatgtaaa agcgggtccc
     8341 ggaaatcttg gtcccccgct caaaatcgat cagaccggtg ctctcaccga tctcccagtg
     8401 cgtcttcggc tggaacccga actcgggctt ggagccgtat tcgccagtca cgcgattggc
     8461 cgtatcatcc gccccgtccg gtgtctcgtc cgagggcata ttgggaatgg agagcatcac
     8521 cgtctcgaag agggaatcga tctccgcacc ttctttttcg agaagcgcca aacggtcgcc
     8581 caaagcacgc atctcgctga tttttatctg tttttcagcc ggatccttga tcgagggaat
     8641 ggccttggaa acggcattcc gctcactgcg catcgactcg atttcgccga caagggtccg
     8701 gcgtttggca tccagctcaa ggagggcatc gatcgaggtg gtatcaccca ctctcgccag
     8761 cgccgctttg actgcctggg ggttttcacg aacaagacgg atatccaaca tggtctgggt
     8821 acacccttct ctgcgagtct ccatggtttc tgcgtaaggc aaaagccaca ctgataaagt
     8881 agcacggaag ggcaccccgg gagaagtccc ctgactggaa gacgcacgga accggatggt
     8941 atcacatttt ttgagcggga caccgtgggt cccgccaaaa tttcagcata cctccgggat
     9001 tggccaacgc catgattgaa aaagaatcgc ggccgtgcaa tactcagtcg agacaccggt
     9061 cacgaacaac gatgagagaa cttgataaag gtggtccctc catggataca cgggcggtct
     9121 ttgcaaaaac ccaaccatca ctctggccat cccccctgat cgactggctg gccctggcat
     9181 cccccgagga atgcttcagg atcaatctga aaatgctcgg gcggagtctg gaccttccac
     9241 tggaaagcat ccttcctccc tttatccag
//
LOCUS       DAOSHE010000028         9230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6377_length_9230_cov_5.029537, whole genome shotgun sequence.
ACCESSION   DAOSHE010000028 DAOSHE010000000
VERSION     DAOSHE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_9230_cov_5.029537"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLD82_01575"
     CDS             <1..195
                     /locus_tag="PLD82_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG64108.1"
                     /translation="RGGLLDVASIAGGLASQTGDIFRSVIGEVEHRLVQWALERTGGN
                     RSEAARLLGINRNTLNSKIL"
     gene            complement(293..865)
                     /locus_tag="PLD82_01580"
     CDS             complement(293..865)
                     /locus_tag="PLD82_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG64109.1"
                     /translation="MQLKGSKTEKNLLAAFAGESQARNRYTFAADVARREGYEQIANL
                     FLETADNEREHAKRFFKYLEGGMVEITATYPAGVIGSTAENLRAAADGEHEEYVGIYP
                     EMARIALEEGFDEIAQQFEEIAKVEAWHEKRYRTLLANVEGGTVFKKADKVLWKCGVC
                     GYIHEGNEPPVTCPACKNGKKFFEVYSETF"
     gene            complement(896..1036)
                     /locus_tag="PLD82_01585"
     CDS             complement(896..1036)
                     /locus_tag="PLD82_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG64110.1"
                     /translation="MDKWECTVCGYIYDPAENNGVAFEKLPDDYVCPECGATKDMFSK
                     LG"
     gene            complement(1159..2808)
                     /locus_tag="PLD82_01590"
     CDS             complement(1159..2808)
                     /locus_tag="PLD82_01590"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOG64111.1"
                     /translation="MKIALAQINPVVGDVTGNCRMILEIARQAARAGAGLVAFPELSI
                     TGYPPRDLLDRPAFIQGVAVALDRLAGDVPEDLAVIVGAPVLQDGVLSNCAVVCLGGR
                     IVHLARKQLLPYYDVFDETRYFSPGRSVSPWIYRGEKLGIMVCEDAWRMPGLPERPYQ
                     HDPVADLAHQGATLFLNLSASPFQQNKRAVRERIALWHARRHGVPFLLVNQVGGNDEL
                     VFDGCSIAAWPDGSVESCRSFGEDLVLVDTENREAGAIDGTEEKTVLRIRSDMDDLHD
                     ALVLGVRDYAKKCGFRQAVLGLSGGIDSALTAVIAVHALGAENVIGVTMPSRYSSQGS
                     LDDSYALAKNLGIRIETIPIEGLVGAFGESLKDVFAGTSPGLAEENVQARIRGTLLMS
                     LSNKFGWLLLTTGNKSELSTGYCTLYGDMNGGLSVLGDVLKTRVYELSRWINRERIVI
                     PEETITKPPSAELRPDQKDSDSLPEYDLLDRVLSMYLEEQMDGAEIMACGLDGDLVKR
                     ILSLVERNEYKRRQAPPVLRVTGKAFGSGRRMPIAARVRYE"
     gene            complement(2845..3138)
                     /locus_tag="PLD82_01595"
     CDS             complement(2845..3138)
                     /locus_tag="PLD82_01595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG64112.1"
                     /translation="MRRNKQMEWRASHILVKDKELAQEVLKRARAGASFSALAKEFST
                     CPSKSSGGDLGWFGAGKMVKPFEDAVKNLANGGTSGLVQTQFGYHIIRKTGER"
     gene            complement(3188..3571)
                     /locus_tag="PLD82_01600"
     CDS             complement(3188..3571)
                     /locus_tag="PLD82_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG64113.1"
                     /translation="MDSLDIQATKSSPLVVFDPVAGVLRIEGQSYPENPVRFYEPVLA
                     ALEEWFVEKAGCALTVNINLSYMNTSSSKCLMNLLDRLDEASRGGALVTVNWYYDVDN
                     EMAHESAEEFREGLQVPFNVIALES"
     gene            complement(3587..4135)
                     /locus_tag="PLD82_01605"
     CDS             complement(3587..4135)
                     /locus_tag="PLD82_01605"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG64114.1"
                     /translation="MRDALLFDLTNTLKRENLLLTFCGPFSQGIIEEIGTALQGYLAA
                     GENPPSHNSSVFSIFIEQTQNIRNYFRSRRIDEFTDPQHGYGIVVIGNDSGRFSISSG
                     NLISNDDLPALADRIRTINALTREELQDLYKQTLRNKRMNEGGGAGLGFIEMARRASQ
                     PLEFATRAVDAGHSFFTLHVVI"
     gene            4277..5134
                     /locus_tag="PLD82_01610"
     CDS             4277..5134
                     /locus_tag="PLD82_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG64115.1"
                     /translation="MTSEPHLTGEEQVIQDAESLLAGGDIHPSEYAALLEQYRKLARQ
                     TEKLVRISDKQQVQLNRVNEELAEKNSILADREAHLVKLVDEKTQKIERITIALVNAL
                     EDANLLNDADTGNHIKRVSKYSAEIARAYGCAPEFVRKIELYASLHDIGKVGIPDEIL
                     KKRGSFEDDEFERMKQHVLIGFRMLDSEDLDSMAKNIVRYHHEKWDGSGYVEGLTGEA
                     IPIEARMVALADVYDALTSRRAYKPAYSESETEDIIRAGAGKHFDPHIVEVFFTRIQA
                     IIAIRDEFT"
     gene            complement(5145..6455)
                     /locus_tag="PLD82_01615"
     CDS             complement(5145..6455)
                     /locus_tag="PLD82_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG64116.1"
                     /translation="MLLLVVGAWSLGAADSWADSVCDTGRNCSWLTDAEKDIIREHNR
                     LRVDPARYAEEVIKPIIGFYEGILLKRPGEIAIRTHEGVGAAQECYQALKAARPAPAL
                     VPSKGITLAARDHAADQSRTGRTGHDGSDGSQMHTRIQRYGSGNTYGENISYGRYQKE
                     IGKRIISQLLIDDGVPSRGHRVNILRESFFSVGVAINTHPNYGMICVLDYAGQFTDKP
                     EVARADPRIPAGFAVTPVSSAASVTSSSSAPSIGPDADREDDDEGTSVRQGRRWRFDD
                     PRGDVTPGYLDIVKTTAQITSEHLIVQIRLASPIREMDVNAQALAKGALEWAWYTVID
                     VNGDGKDDFGMGIYRNKPNNASPARMSSAGLPKPVAWKIQNGKSIMIRTAVDGSVRGR
                     MITLRIPLGNLSGWSGNAERVVVRSRTLWNNGSQVRKDVSEPAR"
     gene            complement(6572..8098)
                     /locus_tag="PLD82_01620"
     CDS             complement(6572..8098)
                     /locus_tag="PLD82_01620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64117.1"
                     /translation="MKSKKSIVRTVVVGTLLAIFVTTAVANLIMYLVDVGSLRKEIDV
                     LAENVVSRLEYGVQKPLWDFDRQQVETLIRLEMGQRDLLAIVIRETNGNIYAGFDRSG
                     GRMVHYTNDATQKGGLDSAIVTGAREIRYQDVTIGTIEVYLSREHHEARTERLLLRFL
                     VQLLAISAAISFVVGFLLKRLVYQPLYGLITVVRRFGERDLAARVHDLRENELGELGR
                     SFNSMAVIIQTYNQQLEQIVAERTRELTDKNEKILESIRYAMRLQESILPDPEMLRRF
                     FSEHFVVWKPKDIVGGDFYWSHETGGNHFLAVVDCTGHGVPGALMTMTVNALLNHIIE
                     DICCDDPALILKQLNLQLKKTLHQEHHENSIDDGLEIGLCLVRPAAGEMLYAGAKLAL
                     LVQSGDGAITVIRGDRQAIGYKRSRLEYDYAMHRIALVPGMRFYLASDGFLDQNGTGH
                     PFGIGLKMFQRFVEAAAPIPLDEQGPYFEEALRAWMGTEIQRDDITLVGFVVRTGERV
                     "
     gene            complement(8109..8879)
                     /locus_tag="PLD82_01625"
     CDS             complement(8109..8879)
                     /locus_tag="PLD82_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG64118.1"
                     /translation="MKSIGLAVCLLCAWISLGVASEKTLVLATAPFPPFRIIQADGSV
                     AGADADIVREVFALAGYRVRYQVYPLARATDLLIWGGVAGFVSVTSSPERERYCHFSL
                     PINHVRDVFFKRKDRTIAWRTMEDLAPYVVGHSGYNYAPVFLDAIRRQVFRRVETVRA
                     ENPEPLQFRKLLAGRMDLFICEMNVGLWFLRNHAPEFSGIDSIATPVGPVRSFHVAFS
                     KRWAGVDELVRQFNQALVQWKQTQRQGLIFSKYGMVGL"
     gene            9043..>9230
                     /locus_tag="PLD82_01630"
     CDS             9043..>9230
                     /locus_tag="PLD82_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64119.1"
                     /translation="MTRLDMSAAELMPRIRTSSIFRYLSDGECLDLVNRGIVTRYEAS
                     ERFITEGEESPTFYVVVGG"
BASE COUNT         2128 a         2976 c         2171 g         1955 t
ORIGIN      
        1 aggggaggac tgcttgatgt tgccagcatc gcaggcggtc ttgccagcca gaccggagac
       61 atcttccgct ccgtcatcgg ggaggtcgaa caccgtttgg tccagtgggc gctggagcgg
      121 acaggaggca accgctcgga agcggcacgg ctcctcggta tcaaccggaa caccctcaat
      181 tccaagattc tctagccagc ctccggaggg caccatacca aaacagggca ccgcgacctg
      241 cgatgccctg ctctcacata tacagtccgg agactgttcc ccaaacaaag actcagaacg
      301 tttccgaata gacttcgaag aattttttac cgtttttaca ggctggacaa gtcacgggcg
      361 gctcgtttcc ttcatggata tatccgcaca ccccgcactt ccagagaacc ttgtcggctt
      421 tcttgaacac ggtcccgcct tcaacattgg ccagcagggt ccgataccgc ttttcatgcc
      481 aagcctcgac cttggcgatc tcctcgaact gctgggcgat ctcgtcaaag ccttcctcaa
      541 gcgcaatcct tgccatctcg ggataaatcc ccacgtactc ctcatgctcg ccatcggccg
      601 ccgccctgag gttttccgca gtacttccaa tcaccccggc aggatacgtt gcggttatct
      661 caaccattcc gccttcaagg tacttgaaaa aacgttttgc atgctcccgc tcattgtccg
      721 cagtctccag aaaaagattg gcaatctgct catacccttc gcgtcgcgca acatccgccg
      781 caaaggtata cctgttccgc gcctgggact ctcccgcaaa cgcagcgagc aggttttttt
      841 ctgtttttga tcccttgagt tgcataggtc ttctccccgc cattcctcgt tcgcatcaac
      901 cgagtttgct gaacatatcc ttggttgcac cacactccgg gcagacatag tcatccggga
      961 gcttctcgaa ggcaactcca ttattctcgg ccggatcgta gatatatccg cagactgtgc
     1021 actcccactt gtccatgacg cactctcctg aaattttctt tttacacccg catcccccgg
     1081 acacggcact cctacaggat acaacagctc cgcccgaagt ccaaaacttt tctgtccgca
     1141 accggctcat ccgccggatc actcatagcg cacccgggca gcaatcggca tcctccgacc
     1201 ggatccaaag gcctttcccg tcacccgcag caccgggggg gcctggcgcc tcttgtattc
     1261 attccgttcc accagactca agatccgttt taccagatct ccatccagcc cacaggccat
     1321 gatctcggcc ccatccatct gttcttcgag atacatcgag agcaccctgt cgagcaggtc
     1381 atattccggc aggctgtcgc tgtccttctg atcgggtctc agctcggctg aaggcggttt
     1441 ggtgatcgtc tcttcgggaa tcacgatccg ctccctgttg atccatcgag aaagttcata
     1501 cacccgggtc ttcaatacgt ctcctaagac tgagagtccg ccattcatgt caccatagag
     1561 tgtgcaatac cctgtcgaga gttcgctctt gtttcccgtc gtcagaagca gccatccaaa
     1621 cttgttcgaa agggacatca ggagggtccc gcgaatccgc gcttgcacat tctcttccgc
     1681 caagcccggg ctggtccctg caaaaacatc tttcagcgac tctccaaatg cccccaccag
     1741 accctcgatc ggtatggtct cgatccggat tccgagattt ttggcaagcg cgtacgagtc
     1801 atccagactc ccctgcgatg aataccggga tggcattgtc accccgatca cgttttcagc
     1861 cccaagggca tgcaccgcga tcacggctgt caaagcggaa tcgatcccgc ccgacaatcc
     1921 gagaactgcc tggcgaaaac cgcatttctt cgcgtagtcc cgcactccaa gcaccagggc
     1981 atcgtgcaaa tcatccatgt cgctcctgat ccggaggacg gtcttttctt cagtgccgtc
     2041 aatcgcgccg gcctcccggt tctccgtatc gaccagcacc aggtcctctc caaaactccg
     2101 gcacgactca accgacccgt ccggccaggc agcaatgctg cacccgtcaa aaaccagctc
     2161 gtcatttccg ccaacctgat tcaccaggag aaacgggact ccatgtctgc gggcatgcca
     2221 gagggcaatc cgttcacgaa ccgcccgttt gttctgctgg aacggtgaag ccgaaagatt
     2281 gaggaagagg gttgcgccct gatgcgcaag atcagcaacc gggtcatgct gatacggtct
     2341 ctcgggcaaa cccggcatcc gccaggcgtc ctcgcacacc atgattccca gcttttcacc
     2401 acgatatatc cagggactga cagaccttcc cggggaaaaa tacctggtct cgtcaaagac
     2461 atcataatat ggcaataact gtttccttgc gagatggaca atcctgcccc ccagacagac
     2521 cacagcgcag ttcgaaagaa ccccatcctg caggacaggc gctcccacga tcaccgcgag
     2581 atcttccggc acatcccctg ccagacggtc aagcgcgacc gccacgccct gaatgaacgc
     2641 cggcctgtcc agcagatccc ggggcggata gcccgtgatg gagagttccg ggaacgccac
     2701 cagcccggcc cccgccctgg ccgcttgccg ggcaatctcg agtatcatcc ggcaattccc
     2761 ggtcacatct ccgaccaccg ggttaatctg cgcaagcgca atcttcatgg gaaactcttt
     2821 ttcattttcc ggaaagaaac gttttcagcg ttcaccggtc ttgcgaatga tatgatatcc
     2881 gaactgggtc tgcaccagac cgctcgtccc gccgtttgcc aggttcttca cggcatcctc
     2941 aaagggtttg accatctttc ccgcaccgaa ccagcccaaa tcaccgccgg aagacttgct
     3001 gggacacgtg gaaaactcct tggccagagc ggaaaacgat gcacccgccc gggcccgctt
     3061 caagacttcc tgggcaagtt ccttgtcctt gaccagaata tgactggcac gccactccat
     3121 ctgcttgttc ctccgtatat tcccgtcgtt ttgtattgaa tatacgaaga ctcccgacag
     3181 ggggtcatca gctctccagg gcaatcacgt tgaacggaac ctgcaatcct tcccgaaatt
     3241 cctcggccga ctcatgggcc atttcattgt ccacatcgta gtaccagttg accgtcacca
     3301 atgccccgcc ccggcttgcc tcatccagtc tgtccaaaag gttcatcagg cattttgaac
     3361 tgcttgtgtt catatacgag aggttgatat taaccgtcag ggcgcatccg gccttttcaa
     3421 caaaccattc ctcaagggcc gcaagcacag gttcgtaaaa acggaccggg ttttccggat
     3481 aggactggcc ctctatgcgc aacacccctg ccaccggatc aaacacgacg agggggcttg
     3541 attttgtagc ctgaatatca agactgtcca tacatacatc tccttgtcag atcacgacgt
     3601 gcagggtaaa aaaactgtgt ccggcatcaa cagccctggt agcaaactca agaggctgcg
     3661 aggcgcgtct ggccatctcg ataaaaccca atccggcccc accaccctcg ttcattcttt
     3721 tgtttctcaa ggtctgcttg tacagatcct gcaactcttc cctcgtcaat gcattgattg
     3781 tccgaatccg gtcagccaac gccggcaaat catcattgct gatcaggttc cctgacgaga
     3841 tggaaaatct tccgctgtcg ttcccgatga cgactatccc gtagccgtgc tgggggtcgg
     3901 taaactcatc aatgcgacga ctccggaaat aatttctgat gttctgtgtc tgctcgataa
     3961 aaattgaaaa aacagacgaa ttatgggacg gaggattctc tcccgcagcc agatacccct
     4021 gcaatgctgt cccgatctct tcaataatac cctgcgaaaa cggtccgcaa aacgtcaaaa
     4081 gcaggttctc ccgcttgagc gtatttgtca gatcaaacaa cagcgcatcc cgcatactct
     4141 cctccaacag gctcgccgga actccccacc cttgcacttg tgccctgtac gcaaacatga
     4201 taccatgacg ccatgtctgt gtacagtttt tcggattcat ccgcaccaca ccagggaacc
     4261 acaacagggg gagtgtatga ccagcgaacc acatcttacg ggcgaagaac aggtcatcca
     4321 ggacgccgag agcctgctcg cggggggaga tattcacccc tcggaatacg ccgcccttct
     4381 ggagcaatac cgcaaactcg cccgccagac cgaaaaactc gtccgtatca gtgacaaaca
     4441 gcaagtccaa ctcaatcgcg tcaacgagga attggcggaa aagaattcca ttctggctga
     4501 ccgggaagcg catctcgtca aactcgtcga cgaaaaaacc caaaaaatcg aacggatcac
     4561 catcgcactg gtaaatgccc tggaagacgc caacctcctc aatgatgccg acacagggaa
     4621 ccacatcaaa cgcgtcagca aatactctgc cgagattgcc cgggcctacg gctgcgcacc
     4681 ggaattcgtc cgtaaaatag agctctatgc ctcactgcac gacatcggga aagtcggcat
     4741 ccccgacgag atcctcaaaa aacgcggctc cttcgaggat gacgagttcg agaggatgaa
     4801 acagcacgtc ctgatcggat tccgcatgct cgacagcgag gatctggact ccatggccaa
     4861 aaacatcgtc cgctatcacc acgaaaaatg ggacggcagc gggtacgtcg aaggactgac
     4921 gggagaagcc attccgatcg aggcccgcat ggttgccctg gccgatgtct atgacgcact
     4981 gacaagccgg cgtgcctaca agccggccta cagtgaatct gaaaccgaag acatcatccg
     5041 ggccggcgca ggaaagcatt tcgacccaca tattgtcgaa gtctttttta ccagaattca
     5101 ggctatcatc gcaatcaggg atgagtttac ctaaaggccc gcccctacct cgccggttcc
     5161 gacacatcct tgcgcacctg gctcccgttg ttccacaggg tacgggaacg gacaactaca
     5221 cgctccgcat ttccgctcca tcccgaaaga ttacccaggg gaatgcgcag cgttatcatc
     5281 ctgcccctga cagaaccatc caccgctgtc ctgatcatta ttgactttcc attctgaatt
     5341 ttccaggcaa ccggctttgg caaccctgca ctgctcatcc tcgcgggact tgcattgttc
     5401 ggtttatttc tgtagatacc catcccgaaa tcatctttcc cgtcaccatt gacatcaata
     5461 acggtatacc acgcccactc caaagcccct tttgcaagcg cctgcgcatt gacatccatc
     5521 tcgcggatcg gagaggcgag ccgaatctgc acgatcaggt gctcgctcgt gatctgcgcc
     5581 gtagtcttca cgatatcgag ataccctgga gtcacatctc cccggggatc gtcaaagcgc
     5641 cagcgccgtc cctgccgaac cgacgttccc tcgtcatcat cttcacggtc tgcatccgga
     5701 ccaatcgagg gggcagacga ggatgacgtc accgaagcag cactcgaaac cggcgtcaca
     5761 gcaaacccgg ccgggatcct cgggtcggcc cgggccacct ccggcttgtc cgtaaactgc
     5821 ccggcatagt caaggacaca gatcataccg taattcgggt gggtattgat cgctacacca
     5881 accgaaaaaa aactctcccg gagtatgttg acccggtgcc ccctgcttgg aaccccatcg
     5941 tcaatcagca attgcgagat gatccttttt ccaatctctt tctgatatct gccatatgaa
     6001 atattttcac catacgtatt gcctgaccca tatcgctgaa tccgggtatg catctgcgat
     6061 ccgtcggatc cgtcatgccc ggtccgcccg gtccgggact ggtcggcagc gtgatccctg
     6121 gctgcgaggg tgatcccttt ggaaggaaca agcgccggtg ccggtcgcgc cgccttgagg
     6181 gcctgatagc actcctgggc tgcacccacc ccttcgtgcg tccggatggc aatctcgccc
     6241 ggccgtttca gcaggatccc ctcgtagaag ccgatgatgg gcttgatgac ctcttccgca
     6301 taccgggcag gatcgacccg cagtctgttg tgctctcgaa tgatgtcctt ttcggcgtcc
     6361 gtcaaccagg aacaattcct ccctgtatca cagacactgt cggcccagct atccgcagcc
     6421 cccagcgacc aggcgccaac caccagcagc aacacgacca accctgcaca cgcaacccgt
     6481 ttccgacaca gcacacccat acctcaatca gcggatttcc ggtctgcaga agcagccggt
     6541 actgcaagag acaacccgac gaaaaaatcg ttcaaactct ttccccggtc cggacaacaa
     6601 atccgacaag ggtaatgtca tccctctgga tttcggtccc catccaggcc cgcaaggctt
     6661 cttcaaagta cggtccctgc tcatccagag gaatcggcgc agccgcctcg acaaagcgct
     6721 ggaacatctt cagcccgatc ccgaatgggt ggcccgtccc gttctggtcg agaaacccgt
     6781 cactggccag atagaacctc ataccgggaa caagggcaat ccggtgcatc gcgtagtcgt
     6841 attccagcct cgaccgctta tatccgatcg cctgccggtc accccggatg accgtgatcg
     6901 caccatcccc ggactgaacc agaagggcca gctttgcccc ggcatagagc atctcgccag
     6961 cggccggacg caccaggcac aaccctattt cgagaccatc atcaatggaa ttctcatgat
     7021 gctcctggtg gagggtcttc ttgagctgaa gattgagctg cttgagaatc aaggcaggat
     7081 catcacaaca aatatcttct ataatatggt tcaacaaagc attcacggtc atcgtcatca
     7141 gcgcaccggg cacgccatgc ccggtgcagt ccacaacggc caaaaaatga ttccctccgg
     7201 tttcgtgcga ccagtaaaaa tcaccaccca caatatcctt tggcttccag acgacaaaat
     7261 gctcgctgaa gaatcttcgc aacatctccg gatccggaag gatcgactcc tgaaggcgca
     7321 tcgcataccg gatgctctcg agaatcttct cgttcttgtc cgtcaattcc ctggttctct
     7381 cggcgacgat ctgctccaac tgctgattgt atgtctggat gatcactgcc atcgagttga
     7441 aactcctgcc aagctcgccc agctcgtttt cccggagatc atgcacgcgc gccgcaagat
     7501 cccgttcccc gaagcgccgg acaaccgtga tcaaaccata cagtggttga tacaccagtc
     7561 tcttgagaag aaacccgacc acaaacgaga tcgccgcgga gattgcaagc aactgcacca
     7621 aaaatcgcag gagcagccgt tccgtccgcg cctcatgatg ctcgcgggag agatagactt
     7681 cgattgtccc gatggtcaca tcctgatacc gtatctcccg cgccccggtc acgatggccg
     7741 aatccagtcc gcccttttgg gttgcgtcat tggtataatg caccatccga cctccactgc
     7801 ggtcgaaccc ggcgtagata ttcccgtttg tttcccggat gacaatcgcg agaaggtctc
     7861 tctgccccat ctcgagcctg atcaaggtct ccacctgctg cctgtcaaaa tcccagagcg
     7921 gcttttgcac accatattca agccggctga caacattctc ggccagcaca tcgatctcct
     7981 tgcgcagaga accgacatcg acgaggtaca tgatcaggtt agccacagcc gttgtcacaa
     8041 aaatcgccaa taacgtcccg acgacaacgg tccgtacgat ggatttcttg ctcttcactc
     8101 cggcctcctc acaaccccac catgccatat ttcgagaata tcaatccctg tcgctgcgtc
     8161 tgtttccatt gcacaagagc ctggttgaac tggcgaacga gctcgtccac acccgcccag
     8221 cgtttcgaga aggcgacatg gaaactgcgc accggaccga ccggtgtagc gattgagtct
     8281 attcctgaaa actcgggagc atgattccgt aaaaaccaga gcccgacgtt catctcacag
     8341 ataaagagat ccatccttcc ggccaggagt tttctaaact gtaacggctc agggttttca
     8401 gcacgaaccg tctccactct gcgaaaaacc tggcgccgga tcgcatccag gaaaaccggt
     8461 gcatagttat acccggagtg tccgacaacg tacggcgcaa ggtcctccat cgtcctccat
     8521 gcaatcgtcc ggtcttttct tttgaagaaa acgtcccgca catgattgat cgggagcgaa
     8581 aaatgacaat accgttctct ctccgggctg ctcgtcaccg aaacaaaacc ggccacccca
     8641 ccccatatca gaagatccgt tgccctggca aggggataca cctggtaccg caccctgtat
     8701 ccggcgaggg caaagacctc ccggacaatg tccgcatcgg ctcctgccac actcccgtcc
     8761 gcttgaatga tcctgaacgg cggaaaaggc gcggttgcaa gcaccagggt tttttcgctg
     8821 gcaaccccga ggctgatcca tgcacagaga agacacactg ccagaccgat cgacttcata
     8881 ggacacctcc ggctctcctc agtatatcac acctacagac ggcatttgcg taacaggatt
     8941 gaatacgata gttctgtccg gttttcctgt cattccgcca gttctccatt cctgtccggg
     9001 acctgccgac atttgaagga tacccctgca aggagcaccc gcatgacccg actggacatg
     9061 agcgccgccg aactgatgcc gcgcatccgc acctcaagca tcttccgcta tctctcggac
     9121 ggcgagtgcc ttgatcttgt caacaggggc atcgtcaccc gctacgaagc cagcgagcgc
     9181 ttcatcaccg aaggcgagga gagcccgacc ttttacgtgg tcgtcggcgg
//
LOCUS       DAOSHE010000029         9010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6584_length_9010_cov_4.603350, whole genome shotgun sequence.
ACCESSION   DAOSHE010000029 DAOSHE010000000
VERSION     DAOSHE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6584_length_9010_cov_4.603350"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLD82_01635"
     CDS             <1..336
                     /locus_tag="PLD82_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64120.1"
                     /translation="YGKPDGVSPPGDTPVSGLTYWGERGGRITVKLHDGTVKDNVIVI
                     DTMDGRFRFANGIGPGSTVEELQAKFPGGNRERGMDYIEWVYNTPGAGFGINGGKVIL
                     VVITSQAKP"
     gene            complement(449..1423)
                     /locus_tag="PLD82_01640"
     CDS             complement(449..1423)
                     /locus_tag="PLD82_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOG64121.1"
                     /translation="MQTETMTDRVACPQCGHESDVQVMPFIDGSDPDQREKFFTGAVN
                     VFRCDSCGTEMMLSSLFEYVDPNLGIALASMPEAAIGHDEFLADFADDGSLLYSDRND
                     DDLALLPLFLRHRRIVFSYAELLLQAEARILRLVTKADEKQGISLPWKAGDTVSQTVT
                     HTCIACKQPFEQEMIVAADVSAKPELREQLAEQRFNAAPCPHCGQVQLIDSAPILWHD
                     PEHFFSVVYYPFGPDRAAKDGILNAAGMVACERIPGSSSSGFAATLAAFTPHEFYHRF
                     LFHEGLAKRQAEKGPTAPRTVTPLSEEDARKALEARFGPGGEHGPSVN"
     gene            complement(1636..3195)
                     /locus_tag="PLD82_01645"
     CDS             complement(1636..3195)
                     /locus_tag="PLD82_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG64122.1"
                     /translation="MGEFILSVQSLSFQHDSASNPVFSGISFGLGMGWHGLVGANGSG
                     KTTLARCIAGLLPPKSGRIHRPGSILYLEQIPEDVPPQLMELLCTPDKAIQAHLSRLA
                     IGHDWPWRWETLSGGEKKRAQIALALASEPDILILDEPGNHLDESARNILIHGLSRWQ
                     GIGILISHDRALLDALTSSTLIMERGGLTRYACSVSTALDQREHNRHHAADQAEQIRS
                     TISRLSVSHGEKKQKVDGSQKRMSRRGLAPKDHDGREKANRARLTGKDRPDAAAMMRL
                     ASRINQLEEELATLPEHGSRKEGVSVWGQPSDKPFWIRRPAGPLPLGDGRTLNLPPLD
                     IARTSRIMLTGENGSGKTTLLTAIMQEIGPVQDVYFMPQEIDTPSALSALDRLAGMTD
                     FEEGQVWSFVARLGTDPKPLRWMRSTAGEHPESIACSPGELRKILLAMAFTQKASLIV
                     LDEPTNHMDLLSVRCLSAAMKDYSGAILAVSHDRAFLGEICTVEWQIDNQAGNLEIMV
                     PFFPEDSRASD"
     gene            3540..4103
                     /locus_tag="PLD82_01650"
     CDS             3540..4103
                     /locus_tag="PLD82_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64123.1"
                     /translation="MVNLDIGRILLEVLASLVIQCTPLDVYPGGVWTWSDGNACWYTI
                     GVERLGESGMTLDFVKYEGEVRTDARLTLGVRRGADGTWAVDRFKRGEDRQWQTVSGP
                     LQAFPGGVVPQAVYIAPWESRQTVMRLVHKEVSPDGQAVVYSYQHGTVDVSVAVIKGQ
                     IARLSMSPDGKRMADAEHLLTREGGNQ"
     gene            4758..5876
                     /locus_tag="PLD82_01655"
     CDS             4758..5876
                     /locus_tag="PLD82_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64124.1"
                     /translation="MLTNETSPEKRTALARSWMAEREGIPWPPYENGQVHVFLWDEER
                     GNRPLPGIALSAHAWLPRAVPFVRLAGTPLLHAGFEAGPLDDIVYWIADGREWYRDPY
                     NPMTAPDPLLHQASRVDLAGRGRPFWASRLPHMLRGRLEKTALGGNWFVRRSAWLYHS
                     PATATNGRGWQDFSGRPSPVDGLLVLEMPSSSDDSVLLANCLDRAFHGSGARVAILCM
                     ESTGSDAPVGGWGFAEIVAKEAPALVFSSGGTPSGLRIGVAATQWNAAHLLHAILRNG
                     PLVQRLWLVAPRLERHSPQLEYARSQNLPGMNGLSVKIGGGEPDGALLERVGESLRGK
                     GARVERQVKQEDWIGLASENPDTRRSALYAIVQDWLRE"
     gene            complement(6061..7707)
                     /locus_tag="PLD82_01660"
     CDS             complement(6061..7707)
                     /locus_tag="PLD82_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64125.1"
                     /translation="MIVRAFLLFLSLTLLATACSSSPEENLITGYFDSLYGEKRSDNL
                     HARMVDFFGDEIKNKRLPVYSAKTRLSEKIKAHSIENWKTTEKDDRLTVSGTITEEAR
                     SGETSRKGFIARFAKQFAPDGKEFIWMLFDIVFSSQGVNDSTAWTDLVTLSKTSKTLV
                     EGITNKITIMLFGKFSEDPVMHILRELAACIPKQVTFAWLDPDIDRGMAASYGVTRAP
                     HIVIENKSRSSVLQVSDLYQDVTGSDGKISRILMAERKLVAALLRVGGQAVDLKLISG
                     IGSREMTGAKGSSITKFVTALSNSGYRLHNVSLADALGTTSTNTNRLDTAKSILIFSD
                     PRQGIPGDLDALLMKRLESGKERAFFLMDTPLTPWGAHLAARFGFAHVNATVVDPANK
                     DSFRGARWIESILYDHESVAHFYRRPNFRVLIAEAAGFVPMQVKPTNFEAVSIVSSSS
                     NGWGETEFDDEKPDEFEYDAGKDIPGPLQLGFAIRSAGTNARPVSIFIGDCDFVANEL
                     SLSRISNWHFASDLLEWLLWPEVHQIRSKPFSAQQFKHLQ"
     gene            complement(7974..8315)
                     /locus_tag="PLD82_01665"
     CDS             complement(7974..8315)
                     /locus_tag="PLD82_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG64126.1"
                     /translation="MSPQQQQRMTRITVAIPAEYHYKNNKGECIIIDFSEGGIAIEAN
                     QILLEGDLLRIKAAPVRNVNLDIWCVVRNVQGRKIGLQFEEISNEMRKQLQDYVYSLL
                     DTNKKSRFESY"
     gene            8478..8834
                     /locus_tag="PLD82_01670"
     CDS             8478..8834
                     /locus_tag="PLD82_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG64127.1"
                     /translation="MAGGSAMGSLAFTTRLEAGVTVLGIGGVIDTGVAPQFEAEMKKA
                     VGGVGKYVGDCSGLEHITSAGIGVLIAMKNAIQSSGGTFVMAGVSEKIMKVFSMLNLD
                     KLVRIYGTVGEAVKAI"
BASE COUNT         1750 a         2320 c         2893 g         2047 t
ORIGIN      
        1 tatggcaaac ccgacggggt cagcccgccg ggagataccc cggtttcggg attgacgtac
       61 tggggtgaac gcgggggccg gatcaccgtc aaactgcacg atggaacggt taaagacaat
      121 gtcatcgtaa ttgacaccat ggacgggcgt ttccgctttg ccaacggaat tggtccgggc
      181 agtactgttg aagagctgca ggccaaattt cccggcggga accgggagag gggcatggac
      241 tacatcgagt gggtgtataa tactccgggg gctggttttg gtatcaatgg gggcaaggtt
      301 attctcgttg tgatcacgtc gcaggccaag ccgtaactgt gaatggccgt gtatgctcag
      361 gcgggcatgg ccaggatgca cccccccccc ttttttttta gtccccgagg gattgaaagg
      421 gggctgaggt tgttccccaa aaagctgttt aattgaccga tgggccatgt tcgccgccgg
      481 ggccaaacct ggcttcgagc gccttgcggg catcctcttc cgagagcggg gtcacagtgc
      541 gcggagccgt ggggcccttt tccgcttggc gcttggccag cccttcgtgg aagagaaagc
      601 ggtgatagaa ttcatggggc gtgaaagcgg caagggtggc ggcaaaaccg gaggaggaac
      661 tccccgggat gcgctcgcag gctaccatgc ctgccgcatt gaggatcccg tctttggcgg
      721 cccggtcggg tccaaagggg tagtagacga cggaaaaaaa gtgctcgggg tcatgccaga
      781 ggatgggggc cgagtcgatc agttggacct ggccgcaatg ggggcagggc gcggcgttga
      841 agcgctgctc ggcaagctgc tcgcgtaatt ccggcttggc actcacgtca gcggcaacga
      901 tcatctcctg ctcgaagggt tgcttgcagg cgatgcaggt gtgggtgacc gtctgcgaga
      961 cggtgtcgcc cgccttccat ggcaggctga ttccctgttt ttcgtccgcc tttgtcacca
     1021 ggcgcaggat gcgggcctcg gcctggagga gcagttcggc atacgaaaag acgatccgcc
     1081 tgtggcgcaa aaagagcggg agcagggcca gatcgtcgtc gttcctgtcc gaatagagca
     1141 ggcttccgtc atcggcaaaa tccgccaaaa attcatcgtg tccaatggcc gcctcgggca
     1201 tcgaggccag ggcgattccc agattggggt caacatattc gaacaggctg gagagcatca
     1261 tctccgtgcc gcaggaatcg cagcgaaaga cattgacggc tccggtaaaa aatttttcgc
     1321 gctggtcagg atcgctgccg tcgataaagg gcatgacctg gacatcgctt tcgtgtccgc
     1381 attgtgggca ggcgaccctg tctgtcatcg tttctgtctg catcttcacg tcctcacgtt
     1441 gttccatcta gtctacgtag gggcggactg tgatgcaagg tgtatgcgtt cgagtgtttt
     1501 ttggtgaaca acctcgagcc tccggggatg tcttgggcgg cctggttgtg gtgggcagtc
     1561 cgcgccggcc tggtggcggt gtcatggtac cgaatttgcc gggctgggta aaaaaacggt
     1621 accatgatcc cggtttcagt cgctggcgcg cgagtcctcc ggaaaaaacg gtaccatgat
     1681 ctccagattc cccgcctgat tgtcgatctg ccactcgacg gtgcagatct cgccgagaaa
     1741 tgccctgtcg tgactgacag ccagaatcgc tccggaatag tctttcatcg cggcggacag
     1801 gcagcggacg gagagaagat ccatgtggtt ggtcggttcg tccaaaacaa tcaaggaggc
     1861 tttttgggta aacgccattg ccagaaggat tttgcggagt tctccgggtg aacaggcgat
     1921 gctctccgga tgctcgcctg ccgtcgagcg catccatcgc aagggcttgg ggtccgttcc
     1981 gaggcgagcg acaaaggacc agacctggcc ttcctcaaag tcggtcatcc ctgcaagccg
     2041 atcgagggcg gagagtgccg acggagtgtc gatctcctgg ggcatgaagt atacatcctg
     2101 gactgggccg atttcctgca tgatcgcggt cagcagggtc gtttttccgg agccattctc
     2161 cccggtcagc atgatccgtg atgtgcgcgc aatatcaaga ggcggcaggt tcagggtccg
     2221 cccgtcaccc agcggaaggg ggcctgccgg gcgacggatc cagaatggtt tgtcggaggg
     2281 ctgtccccag accgagacgc cttcttttcg ggagccatgt tccggcaggg tggccagttc
     2341 ttcctcaagc tggttgatgc gcgatgcaag gcgcatcatg gcggcggcat ccgggcgatc
     2401 cttgcccgtc aggcgtgccc ggtttgcctt ctccctgccg tcatggtctt tcggtgccag
     2461 tccccgccgg gacatccgtt tctgtgagcc atcgaccttc tgtttttttt caccatggct
     2521 gacggacaat cggctgatgg tgctccggat ctgttctgcc tgatccgcgg catgatgacg
     2581 gttgtgttcc cgctgatcca gcgcggtcga gacggagcac gcgtatcggg tcagacctcc
     2641 ccgttccatg atcagggtgg aagaggtcag cgcatccagg agcgcccggt cgtggctgat
     2701 caggatgccg attccttgcc atcgggagag tccgtggatc aggatgtttc tggctgactc
     2761 gtccaggtgg ttgccgggtt cgtcgaggat cagtatgtca ggttcgctgg ccagggccag
     2821 ggcgatctgg gcccgctttt tctctccgcc tgagagggtt tcccatctcc agggccagtc
     2881 atgaccgatg gcaaggcggg agagatgtgc ctgaatggcc ttgtccggcg tacaaaggag
     2941 ctccatcaac tgagggggca catcctcggg gatctgttcg aggtagagta tgctgccggg
     3001 ccgatggatc cggccgcttt tggggggcag caggccggcg atgcagcgag caagggtcgt
     3061 ttttcccgat ccgttggcgc ccacaaggcc atgccagccc attcccaggc caaaactgat
     3121 acccgaaaag acagggtttg atgctgaatc gtgttgaaac gagagagatt gtacggaaag
     3181 aatgaactcg cccatggcgg cctccgggat tgcttgcagg gaagggcacg ccggacacat
     3241 tcaccgaaag gtcgtctgcg tgcctgtcag tgatggatgc gcattggacg gttcgctcct
     3301 cgaaaaaagt attgtttcaa gatatagggt atctgtgtta caggcaagcg gtatgcagtg
     3361 cccgagtctc atccataatc gtggaagaac catgctgtgt cgaaagcagg gtgcgcaggc
     3421 tgaaccaagg agtgtgtcag gtgtatactg aggttcaggc ttgttgtgta cagccgtgaa
     3481 tggaagtcgg actacggtgt agactggtct gtcgcacgac acatatccgg aggaatggca
     3541 tggtcaatct cgatattggc aggattctgc tcgaagtact ggcttcgctt gtgatccagt
     3601 gtaccccgct tgacgtatat ccgggcgggg tatggacatg gagtgacggg aatgcgtgtt
     3661 ggtatacgat tggtgtcgaa cggcttggcg agagcggaat gacgctggat ttcgtcaagt
     3721 acgaaggaga agtccgcacg gatgcacgcc tgacccttgg tgtcaggcgt ggtgcggacg
     3781 gtacatgggc tgttgacagg ttcaaacgcg gggaagatcg acagtggcaa acggtttccg
     3841 gcccgttgca ggcattcccc gggggagtcg ttccccaagc ggtgtatatc gctccatggg
     3901 aaagccggca gacggtaatg cggttggtac ataaggaagt ctcacccgac gggcaggcag
     3961 tggtctacag ctaccaacac gggactgttg atgtttctgt tgcggtgatc aaggggcaga
     4021 ttgcacggct ctcgatgtct ccggacggaa agcggatggc agatgccgag catcttctga
     4081 cgagggaagg tgggaatcag tgaggttctg caggcggggg tggccggtat tgggaatctt
     4141 gtgcctgttt ggtgcctgtg gcctgacatg tgcaccgtct gcccggatgg ttgtcgcaga
     4201 ggttccggtt acgcaaaggg cggtgcgttc ggatgaacgc tggctggtcg gtgtctggac
     4261 gtgggcaggc gaatctcagg aggcagcccg ctggtcaccg gatgccgtac gattcgcgga
     4321 agatggtacc cataccagag ggaggctgca ggccggccga tatgttgcca agccgcattc
     4381 cggttcgacc ccgggcttcg tgagccgctg ggagttgaaa ggcaacagga tttatttctg
     4441 ccttgacgga gccgaggaag ccgccggcat cgtacgtgtt ggagaaaacg tgttccgtac
     4501 agaatttttt gacggaaatg tgcagtacgt cttttggtat gtcaggcaca caggtggtcg
     4561 atgaccgggc ggagggggct ccgatgtcgt atcctcttga tggtcatggt ggctattttt
     4621 tccgggtcat gattgcatgg aatgcacgta tagcccgttt actcattccg gttgggttcg
     4681 gtctcctttg gttgtcttcc tgcgcgccgc aggtacagcc gtttcgcgag cggccaaccg
     4741 tccggacgtt tatcacaatg ctgacgaacg agacatcgcc tgaaaaacgg acggcgttgg
     4801 cgcgctcctg gatggccgag cgtgagggca tcccttggcc gccgtacgaa aatggtcagg
     4861 tacacgtgtt tctctgggat gaagagcgtg gaaaccggcc gcttccggga atcgccctgt
     4921 cggcgcacgc ctggctcccg agggcggtcc cctttgtgcg tctggccggg accccccttt
     4981 tacatgccgg atttgaagcc ggaccactgg atgatatagt ctactggatt gcggatggcc
     5041 gggaatggta ccgggatccc tacaacccga tgacggcccc cgatcccctt ttgcatcagg
     5101 cctcccgggt ggatctggcc gggaggggcc ggccattttg ggcatcgcgt ctgccgcata
     5161 tgcttcgcgg acggctggaa aaaacggccc tcggaggaaa ctggtttgtg cggcgttcgg
     5221 cctggctcta tcattctccg gcgacagcga ccaatggccg gggctggcag gatttttccg
     5281 ggagaccgtc tccggttgac ggattgcttg ttctcgagat gccttcctcc tctgatgatt
     5341 ccgttctcct ggcaaattgc ctcgatcggg ccttccacgg gtccggggcc cgggttgcca
     5401 ttctctgcat ggagtcgacg ggatccgatg cgccggttgg aggatggggg tttgccgaga
     5461 tcgttgcaaa ggaagccccc gctctggttt tttcttcagg aggaacaccc tccggcctgc
     5521 ggattggcgt ggctgccacg caatggaatg cggcccatct tctgcacgcg attctgcgca
     5581 atggccccct ggtccagcgt ctctggctgg tggccccccg actggagcgg cactcgcccc
     5641 agttggagta tgcccgctcg cagaatctcc cgggaatgaa cgggctttct gtcaaaattg
     5701 gcggcgggga acctgacggg gctctccttg agcgggttgg tgagagtctg cgagggaagg
     5761 gtgcgcgcgt agagcgccag gtcaagcagg aagactggat tggtctggcc tcggagaatc
     5821 cggacaccag gcggtccgct ctctatgcga tagttcaaga ctggctgcgc gaataggaag
     5881 gccggccagg atgctgcgtc gccatcccgg cgggtgtccg ggctgcttgg caaccttgga
     5941 cggcaggaag aggatcccgc agtctgcttc atcagccatt cgccgggcag acacgacccg
     6001 gaagccggtt tcgggaggcc gtcagcatga cgccctcccc cgtgcatggt cctccgtgaa
     6061 ttactggaga tgcttgaatt gctgcgcgga aaacggtttt gaccggattt ggtgcacctc
     6121 cggccagagg agccattcaa ggaggtccga ggcgaaatgc caattactga tccgggacag
     6181 tgagagttcg ttggcaacga aatcgcagtc tccgataaag atggataccg gtcgggcgtt
     6241 tgttcctgct gaacggatgg caaatccgag ctgcaatggg ccgggaatgt cttttccggc
     6301 atcatattcg aattcatccg gcttttcatc gtcaaattcg gtttctcccc agccattgga
     6361 ggaactggag acaatggata cggcttcaaa atttgtgggt ttgacctgca tgggaacaaa
     6421 accggctgct tcggcgatca gcacccggaa attcggtctg cggtaaaagt gggcgaccga
     6481 ttcatggtcg tagagaatcg attcaatcca gcgtgcgcca cggaaagagt ctttgttggc
     6541 cgggtcgaca actgtcgcgt tgacgtgcgc gaacccgaac cgtgcggcaa gatgcgcacc
     6601 ccagggggtg agcggggtgt ccatcaaaaa gaaggcccgt tccttgccgg attccagacg
     6661 tttcatcagg agggcgtcaa gatctccggg gatgccctgt cgaggatccg aaaagatcag
     6721 gatgcttttt gccgtgtcga gccggtttgt attggtgctg gtcgtcccga gcgcatcggc
     6781 cagtgagacg ttgtgcaagc gataccccga attggaaagt gccgtcacga attttgttat
     6841 cgaggagcct ttggccccgg tcatttcacg ggaaccgatt ccgctgatga gcttgaggtc
     6901 caccgcttgc cctccgaccc ggagcagagc cgcaacaagc ttccgttcgg ccatcaggat
     6961 acgcgatatt ttaccgtctg atccggtgac atcctggtag agatccgaga cctggagtac
     7021 ggaactccgg gacttgtttt cgattacaat atggggcgcc ctggtgacac catagctggc
     7081 cgccataccg cggtcgatgt ccggatcaag ccaggcaaag gtgacctgct ttggaatgca
     7141 ggcggccagt tcccgaagga tgtgcatgac cggatcctcg gaaaatttcc cgaacagcat
     7201 gattgtgatc ttgttggtga tgccttcaac gagggttttc gaggttttgg agagggtgac
     7261 gagatcggtc caggccgtgc tgtcattgac cccttgggac gagaatacga tatcgaagag
     7321 catccagatg aattctttcc cgtcaggtgc aaattgtttg gcaaagcggg caataaatcc
     7381 cttgcggctg gtctcgccac ttcgagcctc ttcggtgatt gttccgctga ccgtcaggcg
     7441 atcgtctttt tccgttgttt tccagttctc gatgctgtgt gccttgattt tttcggagag
     7501 tctggttttt gctgaataga cggggagtcg tttgtttttg atttcatcgc cgaaaaagtc
     7561 gaccatgcgg gcatggaggt tgtcgctgcg tttttcgcca tagagggaat caaaatatcc
     7621 ggtgatgaga ttttcttccg ggctggatga gcaggcggtg gccaaaaggg tcagggacag
     7681 gaatagcagg aaagcacgaa cgatcatgag agaatcctcc aacagacttg atccggtttg
     7741 gatacagagt ccccccagta tagcagataa tggggcaaat tgtggaagaa aagcgtgaaa
     7801 ctggtgcaaa gagggtcgcc ctccgggtca ggcggaaggc atggaagcgg tcagagagag
     7861 agagtctgtc aaaggccagt ctctgccatt tcaggaagct gtccctcggt ggttttccat
     7921 cctccttccg gtcattgccg gaccggaggg tttcctggct ggatccgtgg cgatcaatag
     7981 gattcgaaac ggctcttttt gtttgtgtcg aggaggctgt agacgtaatc ctggagctgc
     8041 ttgcgcattt cgtttgaaat ctcttcgaat tgcagaccga ttttccggcc ttgcacgttc
     8101 cggacaacac accagatatc gaggttcaca ttccggacag gggctgcttt gatccggagc
     8161 agatcgcctt caaggaggat ctggttggct tcgatcgcga ttccgccttc ggagaagtcg
     8221 atgatgatgc attcgccctt gttgttcttg tagtggtact cggctggaat ggcaaccgta
     8281 atccgggtca tgcgctgttg ttgttgtggg ctcattttta tccatcccct gacctgaacc
     8341 gcaattcgcg ggtgtgatgc atgttcatga atgatttccc gtgaacatgc atcaaatata
     8401 gcccatttat cccgtcttgc agtcaccttc gcaccgttgt atgatgcttt acagattcgg
     8461 gttcgcagag aaccacgatg gcaggaggca gtgcgatggg gagtctggcg tttacaacga
     8521 ggctggaggc cggggtgacg gttcttggta tcggtggggt gattgatacc ggggttgccc
     8581 cccagttcga ggccgaaatg aaaaaagccg tgggtggagt cggaaaatat gtcggagact
     8641 gttccggtct cgaacatatc acgtcggccg gtatcggcgt cctgatcgcc atgaaaaatg
     8701 cgattcaatc ctctggcggg acctttgtca tggccggcgt gagcgagaaa atcatgaaag
     8761 tcttcagcat gctgaacctc gacaagctgg tccgcatcta tggaaccgtc ggggaagcgg
     8821 tcaaggccat ctgaggtgcc ggacgtcctc agattggtcg tgcccgggac cgtcgattat
     8881 ctggatcgca tccgcgactt tgtcggatct gccgccggtg tgtacggctt tgagggtgcg
     8941 acggttgatg aaatcgttct cgccttggat gaggcggttg ccaacgttgt cgagcatgcc
     9001 tacgaaggaa
//
LOCUS       DAOSHE010000030         8946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6651_length_8946_cov_5.699359, whole genome shotgun sequence.
ACCESSION   DAOSHE010000030 DAOSHE010000000
VERSION     DAOSHE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6651_length_8946_cov_5.699359"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLD82_01675"
     CDS             complement(<1..289)
                     /locus_tag="PLD82_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64128.1"
                     /translation="MRHGAGMISLVCVLVLLSAVPMRADMMPYIPDNFKALGPDLGWF
                     WSDNLSGPAIGFDAVTSLYFFTASAGMRQVFSRDDGRDFRGNDGLLSVYLEG"
     gene            564..1628
                     /gene="rlmN"
                     /locus_tag="PLD82_01680"
     CDS             564..1628
                     /gene="rlmN"
                     /locus_tag="PLD82_01680"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG64129.1"
                     /translation="MEPVILLGKNPQELGELTTSLGLPKFAARQLSDWLYKKHATSFE
                     AMTNLAKTARTALAGQAIIGCSEPDSVAVSMDGTKKYLFRALSGDWIETAWIPDEERA
                     TLCVSTQAGCRMGCRFCATARQGYAGNLDAADILNQYRSLPERDTVSNIVYMGMGEPL
                     DNLDAVLASIRACTEDWGLAMSPRRITVSTCGILAPLRRLLDETEVHVALSLHNPFDK
                     ERADLMPITRSQPLEPIFDLLRSYNWRGQRRLTLEYILFDGYNDSEDHFRQLVRLTRG
                     LGDVRINLLRWNTVPGDDGLRGAPAETLELFRDSLNDEGIIATIRRSRGGDIAAACGL
                     LSTARLRNTDAPGVPDANKE"
     gene            1635..4019
                     /locus_tag="PLD82_01685"
     CDS             1635..4019
                     /locus_tag="PLD82_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64130.1"
                     /translation="MATRKTPDASSQSPKPVRTGNTKPRAKKNPDSVTKPAAPRSAPS
                     GFTSGSAPIASPAPAVAKRKPFPVKIFLLGLAALTAGILVFVLAPGNEPLTFIGFALV
                     LGGLLVPTIVLLRRILRKIFFWMPASLMAFAVIFPLTLSAYVVFFFPNETVRQIMQTE
                     MSKALKRPVSIGSLRISLLEGITLDKLVISDRDGKSPFVSASLVLSYKLLPILTGNLV
                     VNQAVLESPSITVTRRIIAGKAVLSIDDLLAGGGAPEAVEEQTATETQSGAAPAIPFA
                     ISVGEIGLRNGSITIEDQATKGFANRYTLDKLDCLASELTWPMTQPLRVRFAFRMTME
                     ELGAPTNTAKALSIHPGIEGRMVLKKSGGTLVPEGRIEFFANNGLFHGQQFLTKAQDF
                     LDTLKADFFRGIQREAEKRLDQFESDLKAMVSKFSGEATKRIDTIIADAQKKFASVTN
                     ELARAGGDAVREFDTSVTSAVTSIDAEVKKLEDEVNAAFSSITRLYPAARTKLKLEMY
                     TSQARAKAETAKKEYAAFGKGLSEGLAKDIAKIIEGERQRFTDWSKSVKNSLDAQITK
                     YAASVRNEFRKQLANVRSFVDSFELDIPFLRKRMEFDEARTGLDITNGVMVFKGLSIG
                     GKEFLVQADGSYGLLDGSITAHLHLSLDKRHAANPLIGIFAAADGRPELGLSIKTVQG
                     NLVFSLDGPPLFERMKSLAADKAQEYIRNYLAQHVTMDAFLSRIGGGNAVTADSGKVA
                     ITAARNSRNTALQNEKARRARMLADESRAIKKKMEDDAKKSVASVLPGGLKKPF"
     gene            4053..5846
                     /locus_tag="PLD82_01690"
     CDS             4053..5846
                     /locus_tag="PLD82_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG64131.1"
                     /translation="MIRPAAVRYGAHVSFDVILFMPAPFTDHPSFPEALIARSLERAG
                     FSVGVIEQPHWQSADDFARCGRPGLFAGIVPGPVDSVALNFTSTGKRRNEDLYQPEGN
                     AYFPDMPPSVSSRIRPDRTLTVFASRIREALGPVPLVIGGLEASLRRFAHWDFQQQAI
                     RRPILLDTRADLVVSGMGELQIVEIARAAARGEILSDADIPGTVRIAKTCPDNPVLLP
                     DAAAVADTPSLLLTQYLDEHRAIREGRPVVQPAGGRFVVSQPPMVYSREDLDAIYGMP
                     FTRAHGWMRDTRPTEGAPTPALLMNRFSVTTHRGCAGGCAFCSIAAHQGRGVVSRSRA
                     SILNEIGAMQRHPDWRGVIGDVGGASAEMWEADCARCQRASCLSPGLCTHYPAPGAQA
                     WQSLLKEIRSLDGIRQVNLGSGVRYEVLLENPSLLEDLLRHHSGQFLRIAPEHTEDHI
                     LSLMKKPPFAVFERFVHLFETIVRRLPRPIRLAPYLIVGHPGETAGDVVRMRDKLKAL
                     GLFGQADVQIFTPTPGSLATAMYCSGQDEHGHTIAVERNMVELVRRQRMLIDSEKSER
                     KPGAPRLDAGRDAPKHGKRKDAPRGGKRFTR"
     gene            complement(5836..6807)
                     /locus_tag="PLD82_01695"
     CDS             complement(5836..6807)
                     /locus_tag="PLD82_01695"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOG64132.1"
                     /translation="MRDRFRAMVHPRQWLLALSMLWVMGGSLPAAAPTPGFINGSSAA
                     SWEFSSGVQSGMIKEYVFSGNYLLSRLDWDIDLLSSLGTALHFRSGHWIFGFSVVLGL
                     PANTGWMKDYDWADRNTDGLTYNGVLSNYSEHDLFMDHALRLELSGYLQLGGFLGGKV
                     WFGASLGWQRYAMQGMDGFAEYPPGSDPVFLTGKVISYTTDQILPIIGLLSEWSSKTL
                     SASLELRGSIIGYERAYDQHHMRSLDFYDYFIFLPSLEVSASLSFAFSTTARWFLSAT
                     LTVFPETRGASYMTDLSVGKSYELTSEGGASSWFLGFETGLQFLISE"
     gene            complement(6843..8510)
                     /locus_tag="PLD82_01700"
     CDS             complement(6843..8510)
                     /locus_tag="PLD82_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG64133.1"
                     /translation="MFLERFVNRTEFSSYDDFFHNFRIHTPDQFNFAYDVVDAIAAET
                     PNKRAMVWVNDHGEKRVFSFADLKRYSDRAANVLRTHGIGKGDAVMMTLKGRYEFWFC
                     ILAIHKLGAIAIPATHMLTEHDLVYRIESADVKMIISVNEQPLVDAIDAAAAHGHPSL
                     TKRLLLGADRPGWVNYDAEMEIAQEVFDRPTGEAAAKTSDIMLLYFTSGTVGYPKMVN
                     HDFAYPLGHIITAKYWQNVQEDGLHYTVADTGWAKAVWGKIYGQWLAGTAIFVHDYDK
                     FDALTLLERASENGVTTFCAPPTIYRFLIKQDLSKFDLSSLKYCVTAGEPLNPEVYEQ
                     FYKATGLRLMEGFGQTEMVVLLATFPWMTPKPGSMGRPSPGYRVVLLNPEGKPCEVGE
                     EGEVAIDLSQGKPWGLFNGYYRDEQRTASVWHDGYYYTGDIAWRDEDGYFWFVGRADD
                     VIKTSGYRVGPFEVESALITHKAVMECAITGVPDPDRGQVIKATVVLAKGFEPSENLK
                     RELQEHVKKVTAPYKYPRIVEFVEALPKTISGKIRRVQIRAEDSNKD"
     gene            complement(8562..>8946)
                     /locus_tag="PLD82_01705"
     CDS             complement(8562..>8946)
                     /locus_tag="PLD82_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG64134.1"
                     /translation="QRLEMDMGTLLTGENPRMNVFTVTRRDQGVRVERRKEYGYQNIA
                     ANFMHKKGEIFVVTVEPKTGFEPHMNAHPGQECDFVLEGRMKVYIHQNEVILESGDSI
                     YFDSSHPHAMEALDGRPARFLAIVL"
BASE COUNT         1943 a         2550 c         2530 g         1923 t
ORIGIN      
        1 tcccctcaag gtatacggag agcagtccgt cattgccgcg aaaatcccgc ccgtcatccc
       61 ggctgaaaac ctgccgcatg ccggcacttg cggtaaagaa atagagtgat gtcacagcat
      121 caaaaccaat agccggtcct gagagattat cggaccaaaa ccagcccaga tcaggcccga
      181 gcgccttgaa attgtccgga atgtacggca tcatgtcagc ccgcatcggt actgctgaca
      241 gcagcacaag cacacacacc agcgatatca tccctgcccc atgtctcatc ctgtccccct
      301 caagcataac tcctgttttc aacagcaaaa acaccctgca accagtatat cccggaggat
      361 gcacgctcac agatcctccg ggcacacgaa gtctaccatg aatattccaa gaagttcgct
      421 gtcggggcat gcgatgcaga cgcacaaaat cgctgccctg gatgacaatt ccggatagag
      481 actgctcgat ggtgcctgta ttcggacaaa taaaatcacg cccgggtatg cctcttcact
      541 ctcccatgag tagcttcttc agcatggaac ctgtcattct tcttggaaaa aatccgcagg
      601 aattgggcga gctgacgacg tcacttggcc tccccaaatt tgccgcgcgc cagctttcgg
      661 actggctcta caaaaaacat gcgacctcat tcgaggccat gaccaatctt gcaaagacgg
      721 cccggaccgc gctggccgga caagccatca tcggctgcag cgagcccgac tcggttgccg
      781 tttcgatgga cggaacgaaa aagtatctct ttcgcgccct gtctggggac tggatcgaga
      841 ctgcctggat tccggacgag gaacgggcga ccctctgtgt ttcgacccag gccgggtgcc
      901 ggatgggatg tcgcttttgt gcgacggccc gtcaggggta tgccggcaac ctggatgctg
      961 cggatatcct gaaccagtac cgcagtctcc ccgaacggga tacggtttca aacattgtct
     1021 acatggggat gggagaaccg ctcgacaatc tggatgcagt cctggccagt atccgggctt
     1081 gcaccgagga ttggggcctg gccatgagtc cgcgccggat cacggtttcg acctgcggta
     1141 tcctcgcacc tctgcggcgt ctcctcgatg agaccgaggt tcatgtcgct ctctctctcc
     1201 acaatccgtt cgataaggag cgggccgatc tcatgccgat cacgcgcagc cagcccctcg
     1261 aaccgatctt tgacctgctc cgctcctata attggcgtgg acaacgacga ctgacgctgg
     1321 aatatattct ctttgacgga tataatgaca gcgaggatca tttcaggcaa ctcgtccgtc
     1381 tgacacgggg cctgggggat gtccggatca atctcctgcg ctggaacacc gttcctggcg
     1441 atgacgggct gcgtggcgct cctgcggaga cactcgaact cttcagggac agcctcaatg
     1501 acgagggtat tattgcgacc atccgccgct cacggggtgg cgatattgcc gccgcctgcg
     1561 gcctcttatc cacagcacga ctcaggaaca cggacgcacc gggtgttcct gatgcaaaca
     1621 aggagtagac aatcatggct accagaaaaa ccccagacgc ttccagtcag tctccaaaac
     1681 cggtacgcac cggcaatacc aagccacggg caaaaaagaa tccggattcc gtaacaaaac
     1741 ccgcagctcc cagatcggct ccttccgggt tcacttcggg ttcggcaccg attgccagtc
     1801 cggcaccggc agttgcaaag agaaaaccct tcccggtcaa aatctttctt ttgggtctgg
     1861 ccgcgttgac tgccgggatt ctcgtttttg tgctggctcc gggaaatgag ccattgacct
     1921 ttatcggatt tgctcttgtt ctcggtgggc ttttggtgcc aaccattgtc ctcttgcggc
     1981 ggattctccg aaaaatattt ttctggatgc cggcatccct gatggccttt gccgtcatct
     2041 ttcccctgac actctcggcc tatgtcgttt tcttctttcc gaatgaaacc gtccgtcaga
     2101 tcatgcagac cgagatgagc aaggccctca agagaccggt ctccattggc agcctgcgga
     2161 tctcccttct tgagggaatc acccttgaca aattggtgat ttcggacagg gatggaaaat
     2221 ccccctttgt cagtgccagt ctggttctct cgtataaact cttgccgatt ctgacgggaa
     2281 accttgttgt caaccaggct gttctcgaat caccatccat cacggtcacg cgaaggatca
     2341 ttgcagggaa ggctgtattg agcatcgatg atcttctggc tggaggaggt gctcctgaag
     2401 ctgtcgaaga acaaacagca actgaaactc aatccggggc agcaccggcg attccctttg
     2461 ccatcagtgt cggagagatc ggcttgcgca acggttcgat cacgatcgag gaccaggcca
     2521 ccaaggggtt tgccaaccgg tacaccctgg acaaactgga ttgcctggcc agcgagctga
     2581 catggccgat gacacaacct ctgcgcgtcc gcttcgcgtt cagaatgaca atggaagaac
     2641 tcggcgcccc gaccaatacc gcaaaggccc tcagtatcca tcccggcatt gaaggtcgga
     2701 tggtcctgaa aaagtccggc gggacacttg tacccgaggg aaggatcgag ttttttgcca
     2761 acaatggtct cttccatggg cagcaatttt tgaccaaggc ccaggatttt ctcgataccc
     2821 tcaaagcgga ttttttcaga ggtatccagc gcgaagcgga gaagcggctg gaccagtttg
     2881 aatccgatct caaggcgatg gtgtcaaaat tctccggaga agcaaccaag cggattgaca
     2941 ccatcattgc tgatgcgcag aaaaaatttg catccgtgac caacgagctt gcccgcgccg
     3001 gaggggatgc tgtccgcgag tttgatacct cggttacttc tgcggtcacc agtatcgatg
     3061 ccgaggtgaa gaagctcgag gatgaggtga atgcggcgtt cagctccatc acccgcctgt
     3121 acccggcggc acggacgaaa ctcaaactcg agatgtatac ctcacaggca agagccaagg
     3181 ccgagactgc aaaaaaggaa tatgcggcgt ttggcaaagg cctttccgag gggctggcaa
     3241 aggatattgc caaaatcatc gagggagaga gacagcgttt tacggactgg tcgaagagtg
     3301 tcaagaacag tctggatgcc cagatcacga aatacgcggc cagtgtccgg aatgaattcc
     3361 gcaagcagtt ggccaatgta cgctcttttg tggacagctt tgagctggat atccccttcc
     3421 tgcgcaaacg gatggagttt gacgaagcca gaaccggtct ggatatcacc aatggcgtca
     3481 tggttttcaa gggcctttcg atcggaggga aggaatttct ggtgcaggcc gatggaagtt
     3541 atgggctcct ggatggaagc atcacggcac atctccatct ttcactcgac aagcggcatg
     3601 cggccaatcc cctgatcggg atttttgccg ccgccgatgg aagaccggaa ctgggtctct
     3661 ccatcaagac cgtacagggg aacctggtct tttctctgga tggaccccca ctgtttgaac
     3721 gcatgaagtc cctggcggcc gacaaggccc aggaatacat ccggaactat cttgcgcaac
     3781 atgtcaccat ggacgcgttt ctctcccgga tcggcggcgg gaatgccgtg actgccgaca
     3841 gcggaaaggt ggcgattacg gcagccagaa attccagaaa cacggctttg cagaatgaaa
     3901 aggcacgtcg cgccaggatg ctggccgacg agagccgtgc gatcaagaag aagatggagg
     3961 acgatgccaa aaaatccgtc gcatcagttc ttcctggtgg actgaaaaaa ccgttttaag
     4021 cagtccacgg tgcagacccg cggcagggcg ggatgattcg tcctgccgct gtacggtatg
     4081 gagcccatgt gtcgtttgat gtcatcctgt tcatgcctgc cccctttacc gaccatccct
     4141 cctttccgga agcgctgatc gcccggtccc tcgagcgcgc cggattttca gtgggtgtta
     4201 tcgaacaacc gcactggcaa tctgcggatg actttgcccg ctgtggccgt cccggactct
     4261 ttgccgggat tgttcccggt cccgtcgatt ccgtggcgtt gaattttact tccacgggga
     4321 agaggcggaa cgaggatctc taccaaccgg aaggcaatgc gtatttcccc gacatgcctc
     4381 cttctgtttc ctcgcgcatc cggccagacc ggacactgac agtcttcgct tcacgcatcc
     4441 gggaggcgct gggtcctgtt cccctggtca tcggaggact tgaggccagt ctgcggcgct
     4501 ttgcccactg ggactttcag cagcaggcga tccgtcgtcc gatccttctc gatacccgtg
     4561 ccgatctggt tgtcagcggg atgggggaat tgcagatcgt ggagatagcc cgggcggccg
     4621 cacgcggaga gatcctctca gacgcggaca ttcccgggac tgtccggatt gcaaaaacat
     4681 gtcctgacaa cccggttctt cttccggatg ccgctgccgt cgccgatacg ccatctctgc
     4741 tcctgacaca gtatcttgat gaacaccgtg ccatccgtga gggacggcct gtcgtgcaac
     4801 cggcgggcgg tcggtttgtc gtcagccagc caccgatggt ctattcccgt gaggatcttg
     4861 atgcgatcta cggcatgccc tttacgcggg cgcatggctg gatgcgggat acacggccta
     4921 cggagggcgc tccgactccg gccctcctga tgaacaggtt ttccgtgacc acccatcgtg
     4981 gctgtgcggg tgggtgtgcc ttctgttcga tcgcagcaca ccagggccgg ggtgttgttt
     5041 cccggagccg ggcatccatt ctcaacgaga tcggggcgat gcagcggcat ccggactggc
     5101 ggggcgtgat cggagatgtc ggtggggcct cggccgaaat gtgggaggcg gattgtgccc
     5161 gctgtcagcg ggcgtcctgt ctctcaccag gtctttgcac acactatccg gcgcctgggg
     5221 cccaagcatg gcagagtctc ctcaaagaaa tccgttctct cgacgggatt cgacaggtca
     5281 atctcggctc aggggttcgc tatgaggtct tgcttgaaaa cccgtcactc cttgaggatc
     5341 tcttgcgcca ccattcgggg cagtttttgc gaatcgctcc ggaacacacc gaggaccata
     5401 ttctcagcct gatgaaaaaa ccaccctttg cggtcttcga acggtttgtc catctttttg
     5461 agaccatcgt ccgcaggctt cccagaccga tccgcctggc tccctatctg atcgtcggac
     5521 atccagggga aacagccggg gatgtcgtcc ggatgcgtga caaactcaag gcgttgggtc
     5581 tttttggcca ggcggacgtc cagatcttca cgccaacacc gggcagtctg gccacggcca
     5641 tgtactgcag cggccaggac gaacacgggc acacaatcgc tgtggagcgg aatatggtcg
     5701 agcttgtacg gagacagcga atgctgatcg actcggaaaa atcggagcgg aaaccggggg
     5761 cgccccgctt ggatgccggc agggatgccc cgaaacacgg caagcggaaa gacgccccaa
     5821 ggggagggaa gcggtttact cgctgatcag gaattgcagg cccgtttcaa atccgagaaa
     5881 ccaggacgag gcaccaccct cggacgtgag ttcataactt tttccgacgg aaagatccgt
     5941 catatagctg gctcctcttg tctcgggaaa gaccgtcagg gttgctgaca ggaaccagcg
     6001 tgccgttgtg gaaaaggcaa aactcagcga ggcgcttact tccagtgaag gcaggaagat
     6061 gaagtagtcg taaaaatcca gcgaacgcat gtggtgctga tcgtaggctc gctcatatcc
     6121 gatgatcgaa ccacgaagct cgagggatgc gctcagggtt ttcgagctcc actccgaaag
     6181 cagtccgatt atggggagaa tctggtcggt cgtatagctg atgaccttac ctgtcagaaa
     6241 aaccgggtcg gacccaggag ggtattcggc aaagccgtcc atgccctgca tggcgtagcg
     6301 ctgccaaccg aggctggctc cgaaccagac cttccctccg agaaatccac ccaactggag
     6361 atacccgctc agttcaagac ggagagcgtg atccataaag aggtcgtgtt cggagtaatt
     6421 cgagagaact ccgttatagg tcagcccgtc agtatttctg tcagcccagt catagtcttt
     6481 catccagccg gtatttgccg gcaatccaag aacgaccgaa aagccgaaga tccagtgccc
     6541 ggaccggaaa tgcaaggccg tcccgaggct cgacagcagg tcgatatccc agtccagccg
     6601 gctgaggaga tagttgccac tgaaaacgta ttccttgatc atcccggact ggactccgct
     6661 cgagaattcc caggaagcag ctgacgaccc gttgataaat ccgggggtcg gggccgccgc
     6721 ggggagactc ccacccatga cccagagcat gctcagagcc agcagccact gtcggggatg
     6781 caccattgcc cgaaacctgt cacgcatgaa ccgctccttg tccgcctgag gatattcctc
     6841 aatcaatcct tgttgctgtc ttctgcccgt atctggactc gacggatctt gccgctgatc
     6901 gtcttgggaa gggcttcgac aaattcgacg atgcgcgggt atttgtaggg tgcggtgacc
     6961 tttttgacgt gctcctggag ttcccgcttc aaattctcgc ttggctcgaa tcccttggcg
     7021 aggaccacgg tggccttgat gacttgacca cggtcaggat ccgggacacc ggtgatggcg
     7081 cattccatga cggccttgtg ggtgatcagc gcgctctcga cctcgaacgg gccgacacgg
     7141 tacccggagg ttttgatcac gtcgtcagcc cgaccgacaa accagaaata cccgtcttca
     7201 tcccgccagg caatgtctcc ggtatagtaa tacccgtcat gccagaccga ggctgtccgc
     7261 tgttcatccc ggtaatagcc gttgaagaga ccccagggtt tgccctgtga cagatcaatg
     7321 gcaacctcgc cttcctcgcc gacttcacag ggtttgccct cggggttgag gagcacaaca
     7381 cgatagcccg gagatggtct gcccatggag ccgggcttgg gcgtcatcca ggggaatgtc
     7441 gccaaaagaa ccaccatctc ggtctgtccg aagccctcca tcaggcggag tccggtggcc
     7501 ttgtaaaact gctcgtacac ttcggggttg agcggttcac cggctgtgac acagtacttc
     7561 agggatgaga gatcgaactt ggagagatcc tgcttgatca aaaagcgata gatcgtgggc
     7621 ggggcacaaa aggtcgttac cccgttttcg ctggcacgct caagcaatgt caacgcatcg
     7681 aatttgtcgt agtcatgcac aaagatggcc gtgccagcca gccactgacc gtagatcttg
     7741 ccccagaccg ccttggccca accggtatcg gccaccgtgt aatgcagccc gtcttcctgg
     7801 acgttctgcc aatacttggc cgtgatgata tgcccgaggg ggtaggcgaa atcgtggttg
     7861 accatcttgg gatacccgac tgttccggac gtgaaataga ggagcatgat gtcgctggtt
     7921 ttcgcggcag cctcaccggt cggccggtca aagacttcct gggcgatctc catctcggca
     7981 tcataattga cccagcccgg acgatcggca ccgaggagaa gccgctttgt gagggaaggg
     8041 tggccatggg cggctgcggc atcaatggca tccacgaggg gctgttcgtt gacggagatg
     8101 atcatcttga catcggcgct ttcgatccgg tagacgagat cgtgctccgt cagcatgtgg
     8161 gtggccggga tggcaatcgc gcccagcttg tggatggcca gaatgcaaaa ccagaattcg
     8221 tagcggccct tcaaggtcat catcacggca tcacccttgc cgatcccgtg tgtgcggagg
     8281 acgttggccg cccggtcgct atagcgcttg agatcggcaa acgagaagac ccgcttttca
     8341 ccatgatcgt tgacccagac catggcccgc ttgttgggag tctcggcagc gatggcgtcg
     8401 acaacgtcgt aggcaaaatt gaattggtcg ggggtatgaa tgcggaaatt atggaaaaaa
     8461 tcatcgtatg acgaaaattc ggttctgttg acaaaacgct ccagaaacat atgtgtatcc
     8521 ttccggttaa agttgggtgt gttgggtctc tatggcagat gttacagcac gatggcgagg
     8581 aagcgtgccg gtcgtccgtc cagcgcttcc atggcgtgcg ggtgtgaaga gtcaaagtag
     8641 atgctgtcgc ccgattcgag gatgacttcg ttttgatgaa tatagacttt catccgcccc
     8701 tcaagcacaa agtcgcactc ctgcccggga tgggcattca tgtgcggttc aaatcctgtc
     8761 ttgggctcga ctgtgacgac gaagatctcg ccttttttgt gcataaagtt ggctgcaatg
     8821 ttttgatatc cgtattcctt gcgccgttcg acccgcacgc cctggtcacg ccgggtcacg
     8881 gtaaacacgt tcatccgggg attttctcct gtcaggaggg tgcccatatc catctcgagg
     8941 cgctgg
//
LOCUS       DAOSHE010000031         8852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6725_length_8852_cov_4.617483, whole genome shotgun sequence.
ACCESSION   DAOSHE010000031 DAOSHE010000000
VERSION     DAOSHE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_8852_cov_4.617483"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..290
                     /locus_tag="PLD82_01710"
     CDS             87..290
                     /locus_tag="PLD82_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOG64135.1"
                     /translation="MIKVTRLNGSEFWINPHQIEFMEETPDTVIKMFSEKKIVVRESV
                     AEVNARIMEYRRQLGAIGNDSTF"
     gene            321..1100
                     /locus_tag="PLD82_01715"
     CDS             321..1100
                     /locus_tag="PLD82_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG64136.1"
                     /translation="MDLGTVGGIIGGFAAIIISVFWGGGTLDTYIDIPSVFITVVGCF
                     CSVLISYPLKKVFGMVGVLKHAFFTKKEDPAHIIDMLVTFSEKARREGLLALEDDVDE
                     VSDNFLKKGIQLVVDGTDPELVRNLMSTDLNNIEARHMEGINILVFGAAVAPAYGMIG
                     TLIGLIAMLKNIEDKSSLGPNMAVALITTMYGSVMANLIFQPLATKLDGNHRREMIMK
                     EIMIEGTLSIQQGDNPRIVKEKLASYLPPAQREALGGDDRD"
     gene            1104..1826
                     /locus_tag="PLD82_01720"
     CDS             1104..1826
                     /locus_tag="PLD82_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG64137.1"
                     /translation="MGKKSEKKSYAAPEYMLTYGDMVTLLLTFFVAMFTFGEADPREM
                     RLILSSFSGNLGVFEGGMTLSKGQLADMGMSIESLPSTERGSSMARSVKQAQEILKPE
                     IRSKMVRVSEDERGIVISLASDTFFGPGSARLNLERGADLLKKVVQLVERATNQKIRI
                     EGHTDDTPVAEGGAGEERYRDNWQLSTARALSVLGYMTKERVPDERLQVGGYAENKPI
                     ESNDTPEGRAYNRRVDIVLLRD"
     gene            1844..2410
                     /locus_tag="PLD82_01725"
     CDS             1844..2410
                     /locus_tag="PLD82_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOG64138.1"
                     /translation="MPEDMDDFNFGDDEGGEGEGAQAEAASGGGGLKRFLSGTILKVL
                     MYVAAGIAVFIIALFAGKMGGGSAAKDDAYAQDQKALEQKAPPLATFELKEFMSNTAD
                     VDTTHFVRMKLSLGYEKNNLLLQTELSERRMQIFDLIMLTLNGKRKEDLDSAQKKQDL
                     KDELIKKINGLLQNGKIDDIYYAEFSVN"
     gene            2561..3607
                     /gene="fliM"
                     /locus_tag="PLD82_01730"
     CDS             2561..3607
                     /gene="fliM"
                     /locus_tag="PLD82_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOG64139.1"
                     /translation="MTEVLSQEEIDALLSAITTDGETQTASMDLSTRVATEQRKVKIY
                     DFKRPDKFSKDQIRTLQMMYENFARTTTTSLSALLRTMVHVHVATVDQLTYEEFIRSI
                     PSPTTLAVINMDPLKGSAIFEIDPAITFTVIDRLFGGSGESAKITRELTDIEQSVMEN
                     IIVRILSNLREAWANVIDLRPRLGDIETNPQFAQIVPPNDMVVLVTIETKVGTVEGMM
                     NFCIPYITIEPIISKLSAQYWYSSIRKGTTSENLNVIKDRLDTVDIVVSAELGSVDIM
                     TNDLLSLAAGDTILLPNSKINSDMVIKIGDRPKFKCRPGIVGSRMAVQVTGKLEDITE
                     ESLEEIALGGDDNE"
     gene            3600..4715
                     /gene="fliN"
                     /locus_tag="PLD82_01735"
     CDS             3600..4715
                     /gene="fliN"
                     /locus_tag="PLD82_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOG64140.1"
                     /translation="MSDGSLSQDEIDALLRGTEELMGGGSPATAAATGFTAPTASGES
                     LSDVEKNSVLDVLRAATEAAASSMGALLSNKVSVGMMGVEVMRPEEIRSELGGGLVQV
                     TMNYADGVSGENIFVLPDSAALMVANIAMGQNSQELTPMVLSTLAQTMTQAWGAAASN
                     MSARLGRAVRFSEARAEAAHGNTALTLPGGGSMLRIGYQLRVEGQNPAQVSQIMSMKG
                     AREIINAAMGIGADMSFGSMGAGAPMGGGFAGGMSMPGAAQQTAAQAVGISNVQYPQL
                     QQNMGAGQMPHNISVLMDVQMQLSVELGRTRKKISEILSFGEGSIIELDKLAGEPVDV
                     LVNGKLIAKGEVVVIDENFGVRVTEIVSPNSRLEYTT"
     gene            4806..5396
                     /locus_tag="PLD82_01740"
     CDS             4806..5396
                     /locus_tag="PLD82_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOG64141.1"
                     /translation="MRFFVLLMALLVPALLAGAPQQATNQAGLGAINQRMQELAGSGS
                     RGELAAPGGLGTFVETILVLALFIAGIYGVFRFVQRKRNMGDAGEDAIRVLANRSAGG
                     SRAVQVIEVGRRVFVIGIGDSAINLIAEIEDEETLESLRLDHARGNAAQGEGFFERLV
                     GMLGRSKSAVRGQVSREKLEYLRAQKERLARLKKEP"
     gene            5468..6241
                     /gene="fliP"
                     /locus_tag="PLD82_01745"
     CDS             5468..6241
                     /gene="fliP"
                     /locus_tag="PLD82_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729300.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOG64142.1"
                     /translation="MSVVTKRSVLALVVLGIVALQTGSLQAQAGPNFVLPKITFGVEQ
                     AQRPGEVAVAMQVLLLLTVLSLAPAFIMMVTSFIRISIVFSFTRMALATQQMPPTQVL
                     MGLALFLTIFVMAPTIKKVNEDALQPFLAQKIGADQFYEKGVAPIRDFMFRQTRENDI
                     ALFMHLSKEPRPATRNDIPTYVLVPAFIVSEMTTAFKIGVLIFVPFIIIDMVVASVLM
                     SMGMIMLPPVMVSLPFKIILFYLVDGWNLLIRKLVEGFV"
     gene            6251..6520
                     /gene="fliQ"
                     /locus_tag="PLD82_01750"
     CDS             6251..6520
                     /gene="fliQ"
                     /locus_tag="PLD82_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOG64143.1"
                     /translation="MDFDLIIRIVRDSLLQILLLSAPMLGISMFVGLLISIFQATTSI
                     QEQTLTFVPKIISIFLALIVFGNWMLATLIDFSRRLFDMIPLMTR"
     gene            6523..7302
                     /gene="fliR"
                     /locus_tag="PLD82_01755"
     CDS             6523..7302
                     /gene="fliR"
                     /locus_tag="PLD82_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOG64144.1"
                     /translation="MIDTGLVIQFQVFLLVFARIFGMMTLAPFFSSLAIPFRFRAILA
                     LLVTMIMILPVLKLPIRPEISLVHYILQLVGELLVGIAIGFLVGVFFAALSFAAELFS
                     MQMGLSITNVFDPQAQIEVPVLGQLLSLFGILVFIAVGAHHHLLNGIWSSYDSLPMLE
                     ISRHSGPFAQGAAAAFTGMFGVAIKIALPMIGVSFLLSIAMGILAKTAPQLNILMLGF
                     PIQILVGFLVLVASLPFLFRFMGNVISESVAFAVKVMGGGS"
     gene            7299..8462
                     /gene="flhB"
                     /locus_tag="PLD82_01760"
     CDS             7299..8462
                     /gene="flhB"
                     /locus_tag="PLD82_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOG64145.1"
                     /translation="MMEERLFLVNTLLPPEGRFLLDLQFFAAEDEGRTEDPTESRKRK
                     AREEGKVPKSQDMSAALVLLVVFWTLAFFGSHMVTGFREAFTYCIDNMYTLEISTNSL
                     RTLFLHFVLLFFEILAPILIMAIIIGVAANVMQVGFLFTWKPVKPELSRISFTPQKIF
                     QRVLFSKQAMVNLVKSVLKIVVIGIVAWLIIEGELPKMINLMDVGVAGAMDSITNVVF
                     VMVNATCLALLALSVPDYIFQRRQHLESLKMSIPEIKEEHKMEEGDPLIKSRIMQKSR
                     ELAQRNLPAMVKKADVVITNPTHLSVALQFDMNIHDQPVVVAKGADHLAFVIRQIATE
                     AGVPIMENKPLARALYHDVNLGDPIPESLFSAVVTVYQRLEKFRNRDYSGVRA"
     gene            8514..>8852
                     /locus_tag="PLD82_01765"
     CDS             8514..>8852
                     /locus_tag="PLD82_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOG64146.1"
                     /translation="MAEPGRKGLQADIMLAVAVIAVVFMLIIPLPTMMLDMLIAVNLM
                     ISLIVLLTVMYMKNAADFSIFPSLLLLTTVFRLALNVSTTRVILSEGMQFDGAIIRAF
                     GEFVVEGNYVV"
BASE COUNT         1857 a         2451 c         2521 g         2023 t
ORIGIN      
        1 cgtgtcacct tgatcatgcc gcccctcccg ccgatccggt tggctatact gtcaggggta
       61 tcagacggat cggcgggagg ggcggcatga tcaaggtgac acgattgaat ggcagtgaat
      121 tctggattaa tccgcatcag attgagttta tggaagaaac tcccgatact gttattaaaa
      181 tgttctccga aaaaaagatt gtggtcaggg agtcggttgc cgaggtaaac gcgcgtatca
      241 tggaataccg caggcagctg ggagcgatag ggaatgactc gacattttga cgatgtcgtg
      301 acgagggcgg gagaggcaga atggatctcg gaacagtagg tggtatcatt ggcggatttg
      361 ccgccattat tatttcggtt ttttggggcg gaggcacgct tgatacctat attgacattc
      421 catccgtctt tatcacggtt gtgggatgtt tttgttctgt tttgatctcc tatccattga
      481 aaaaagtctt tggcatggtc ggtgttctca agcatgcctt ctttaccaaa aaagaggatc
      541 cggcccacat cattgacatg ctggtcacct tttcggaaaa ggcccgtcga gaggggcttt
      601 tggcgctcga ggatgatgtg gatgaggtgt ccgacaactt cctcaaaaag ggaattcagc
      661 tcgttgttga tggaacggat cccgaacttg tccgcaacct gatgtccacc gatctcaaca
      721 atatcgaggc gcgtcacatg gagggcatca atattcttgt cttcggtgcg gctgtcgccc
      781 cggcgtacgg aatgatcgga accctgatcg gtctgatcgc catgttgaaa aacattgagg
      841 acaagtcgtc actcggtccc aacatggccg tcgctctcat cacgaccatg tacggatcgg
      901 tcatggccaa cctgattttc cagcctttgg caaccaagct ggatggcaat catcgacggg
      961 agatgatcat gaaggagatc atgatcgagg gaaccctctc catccaacag ggagacaacc
     1021 cgcgtatcgt caaggaaaaa ctcgcatcct atctgccacc ggcccagcgc gaggctcttg
     1081 gcggggatga cagggactga gacatgggga aaaagtcgga gaaaaagtca tacgcggcgc
     1141 cggagtacat gctcacctac ggggacatgg tgacgctcct tctgaccttt ttcgtggcca
     1201 tgttcacctt tggcgaggcc gatccccggg agatgcgcct gattctctca tccttttcgg
     1261 ggaacctggg tgtctttgag ggcgggatga ccctctccaa gggacagttg gccgacatgg
     1321 ggatgtccat cgagtcactc ccttccacgg aacgaggaag ctccatggca cgttccgtca
     1381 aacaggcaca ggaaatcctc aaaccggaga tccgctccaa gatggtgcgc gtgagcgagg
     1441 atgagcgggg gatcgtcatc agtctggcct cggatacttt ttttggaccc ggatccgcac
     1501 gactgaatct cgaacgggga gccgatcttc tcaaaaaggt ggttcagctt gtcgaacggg
     1561 ccaccaacca aaaaatccgg atcgaagggc atacggatga tacgcctgtt gccgagggtg
     1621 gtgccggtga ggaacgctat cgggacaact ggcagctttc aaccgcccgt gccctgtccg
     1681 ttctcggata catgaccaaa gagcgcgtac cggacgaacg gctccaggtc gggggctatg
     1741 ccgaaaacaa acccatcgaa agcaatgata cgcccgaggg gcgtgcctat aacagacgcg
     1801 ttgatatcgt actgctgcgt gattaagcac aaggagccga aagatgcctg aagatatgga
     1861 tgattttaat tttggtgacg atgagggcgg agaaggcgag ggggcccagg cggaggctgc
     1921 cagtggtggc ggtggactca agcgtttttt gtccgggacc atcctcaagg tcctcatgta
     1981 tgttgcggcc gggattgccg tatttatcat cgcacttttt gcaggaaaga tgggaggcgg
     2041 atcggcagcc aaggatgatg cctatgccca ggatcagaaa gcccttgagc agaaggctcc
     2101 tccgcttgcg accttcgaac tcaaggagtt catgagcaat actgccgatg tcgataccac
     2161 ccattttgtg cgcatgaagt tgagtctcgg ctacgagaag aacaacctcc tcctgcagac
     2221 cgaactctcc gaacggcgga tgcagatctt tgacctgatc atgctgacct tgaatggcaa
     2281 acgcaaggaa gatcttgatt cggcacagaa aaagcaggac ctcaaggacg agctgatcaa
     2341 aaagatcaac ggactcctgc aaaacggaaa gattgatgac atctattatg ccgagttctc
     2401 ggtcaactga tttgcaggaa agtactcaga ttgtcaaaca aagctggcga caatagcagg
     2461 agaaggcgct tttgccttgc atggtcggtg ccttccttgg tagattttcc gtggatcgtc
     2521 atggtccggc ggggacgtct ttgaggatgg agagcggacg atgaccgaag tcttgtcaca
     2581 ggaagagatc gatgccctgc tttcggccat caccaccgat ggcgagacgc aaacggccag
     2641 catggatctc tccacccgcg tggcgaccga acagcgcaag gtcaagatct acgacttcaa
     2701 gcggccggac aagttctcca aggaccagat ccgtaccctg cagatgatgt acgagaactt
     2761 cgcccggacc acgaccacca gtctctcggc cctcttgcgg accatggtcc atgtgcatgt
     2821 cgccaccgtg gaccagttga cctacgaaga gttcatccgt tcgattccca gcccgaccac
     2881 gctggccgtc atcaatatgg accccctcaa agggagtgcc attttcgaaa tcgatccggc
     2941 catcaccttt accgtcatcg accggctctt tggcgggagt ggagagtccg ccaagatcac
     3001 ccgcgagctg accgacatcg aacagtcggt catggaaaat atcatcgtcc ggattctttc
     3061 caacctgcgc gaggcctggg ccaacgtgat cgacctccgt ccgcgcttgg gtgacatcga
     3121 gaccaacccc cagttcgcac agatcgtgcc tcccaatgac atggtcgtgc tcgtcaccat
     3181 cgagaccaag gtcggaaccg tcgaagggat gatgaacttc tgtatcccgt acatcacgat
     3241 cgagccgatc atctccaagc tctccgcgca gtactggtac tccagtatcc gcaagggaac
     3301 aaccagcgag aacctcaacg tcatcaagga cagactggat acggtggaca tcgttgtcag
     3361 tgccgaactc ggctcggtag acatcatgac caatgatctt ctctcgcttg cagcagggga
     3421 taccattctc ctgccgaatt caaaaatcaa ctcggacatg gtcatcaaga tcggtgaccg
     3481 gccgaaattc aaatgccgtc cgggtattgt gggcagtcgc atggctgtcc aggtcaccgg
     3541 gaaactcgag gatatcaccg aagaatcgct cgaggagatc gcattaggag gggatgacaa
     3601 tgagtgacgg ttcgctgtcc caggatgaaa ttgatgccct gttgcgtggt accgaggagt
     3661 tgatgggcgg aggcagcccg gcgacggcgg ctgctaccgg gtttaccgct ccgaccgcgt
     3721 cgggggaatc cctgagtgat gtcgagaaga acagtgtcct cgatgttctg cgggccgcca
     3781 ccgaggcggc ggcctcgagc atgggggctt tgctctccaa caaggtctct gtgggaatga
     3841 tgggggtcga agtcatgcgc cccgaggaga tccgcagcga gctgggaggc ggcctagtcc
     3901 aggtgaccat gaattacgcg gacggtgtca gtggtgagaa tatctttgtc ctgcccgact
     3961 cggccgcctt gatggtggcc aatatcgcca tgggacaaaa cagccaggag ctgaccccga
     4021 tggttctttc gaccctggcc cagacgatga cccaggcctg gggtgcggcc gcgtccaaca
     4081 tgtcggcccg gctcggacgt gccgtccgtt tcagcgaggc gcgtgccgag gcggcccacg
     4141 ggaacacagc cctgaccctg ccaggcggcg ggagcatgct gcggatcgga taccagcttc
     4201 gggttgaggg gcaaaatccg gcccaggtca gccagatcat gtccatgaag ggtgcccggg
     4261 agatcatcaa tgccgcgatg ggaatcgggg cggacatgtc gttcggttcg atgggtgccg
     4321 gcgccccaat gggtggcggt tttgccggag ggatgagcat gcctggagca gcgcagcaga
     4381 cggcggcaca ggccgtcggg atctccaacg tgcagtatcc ccagctccag cagaatatgg
     4441 gtgcgggaca aatgccgcac aacatcagtg tcctgatgga tgtccagatg cagctctcgg
     4501 tcgagcttgg ccggacccgc aagaagatct cggagattct ctcattcggg gaggggtcca
     4561 tcatcgagct ggacaaactc gccggtgaac cggtggacgt tctggtcaac gggaagctca
     4621 tcgccaaggg agaggtcgtg gtcattgacg aaaactttgg agtgcgcgtg accgagattg
     4681 tctcacccaa cagccgcctc gaatacacaa cctgaggtga ggcagcccgg ctttcttgtg
     4741 ataaaaaaaa gacttgcagt cttcccctca gatgcactat acttcccgtg cattctggag
     4801 gggaaatgcg tttttttgtg ctattaatgg ctctgcttgt tccggccctg cttgcaggcg
     4861 ctccacaaca ggcgacaaac caggcaggtc ttggtgccat caaccagcgt atgcaggaac
     4921 tggcgggatc cggttcacgc ggcgagttgg cggctcctgg cggtcttggg accttcgtcg
     4981 agacaatcct tgtccttgct cttttcatag ccggcattta cggcgtgttt cgctttgtac
     5041 agcgcaagcg gaacatgggt gatgcgggcg aggatgccat ccgggttctt gccaaccgca
     5101 gtgcgggcgg gagccgggct gtgcaggtga tcgaggtcgg acgcagggtg ttcgtcatcg
     5161 gtatcggcga ctctgcgatc aatcttatcg ccgagatcga ggatgaggag acgctggagt
     5221 cgctccggct ggatcatgcc cggggcaacg ctgcgcaggg tgagggcttt tttgagcgtc
     5281 tggtcgggat gctcggtcgc tccaaatccg ctgtccgcgg gcaagtctcg cgtgaaaagc
     5341 tggaatacct gcgagcccag aaagagcgcc tcgcccggct caaaaaagag ccataacccc
     5401 tcccgaagag acaggatgat gaagtcgagc gacggatacg gtgctggccg tatccctggg
     5461 gggatgtatg tcggttgtaa ccaaacgaag cgtactggca ctggttgtgc tggggatcgt
     5521 tgcgctccag accgggagtc tccaagcaca ggcgggccct aatttcgttt tgccaaagat
     5581 caccttcggt gttgaacagg cccagcgccc tggcgaggtg gccgtggcca tgcaggttct
     5641 gcttcttctg accgttcttt cgctggcacc ggccttcatc atgatggtta ccagcttcat
     5701 ccggatctcg atcgtgtttt ccttcacccg gatggctctg gctacccaac agatgccgcc
     5761 cacacaagtg ttgatgggac tggcgctctt tctgaccatc tttgtcatgg ccccgacgat
     5821 caaaaaagtg aatgaagacg ccctgcaacc ctttctggca cagaaaatcg gtgcggacca
     5881 gttttacgaa aagggtgttg ccccgatccg ggatttcatg ttcaggcaga cccgcgagaa
     5941 tgacatcgcc ctcttcatgc acctttccaa ggagccccgg ccagcgacac ggaatgacat
     6001 tccgacctat gttctggttc cggcctttat cgtctccgaa atgacgacag cattcaagat
     6061 cggggtgctg atctttgtcc ccttcatcat cattgacatg gtggttgcgt cggtgctgat
     6121 gtccatgggc atgatcatgt tgccgcccgt catggtctcc ctgccgttca aaatcatcct
     6181 tttctacctc gtcgatggct ggaatctcct gattcgcaag ctggtcgagg gatttgtcta
     6241 ggagggccct gtggattttg acctgatcat ccggatcgtg cgggattcgc tcctccagat
     6301 cctgcttctc tcggcaccca tgctcgggat cagcatgttc gtcggtcttt taatctccat
     6361 ctttcaggcg acaacctcga tccaggagca gacactgacc tttgttccca aaatcatttc
     6421 gatttttctg gcgctgatcg ttttcggcaa ctggatgctt gccaccctca tcgatttctc
     6481 gcgccgtctc ttcgacatga tcccgctcat gacgaggtaa ccatgatcga cacggggctt
     6541 gtcatccagt tccaggtatt tcttctggtc tttgcccgga tcttcgggat gatgaccctt
     6601 gccccgtttt tttcttccct ggcgataccc ttccgcttca gggccatttt ggctctcctt
     6661 gtgaccatga tcatgatcct gcccgttctc aagctcccga tccgacccga gatttcgctg
     6721 gtgcactaca tcctccagct ggtcggggaa ttgcttgtcg ggattgccat cgggtttctt
     6781 gtcggtgttt ttttcgcagc actctcgttt gctgccgagc tcttttcgat gcagatgggt
     6841 ctgagcatca ccaacgtctt cgacccgcag gcgcagatcg aagtccccgt tctcggccag
     6901 ctgctctctc tcttcgggat actcgtcttc atcgccgtcg gtgcgcatca ccatctcctc
     6961 aacgggatct ggagcagcta cgacagtctc ccgatgctgg aaatctcccg tcattccggc
     7021 cccttcgcac aaggtgcggc ggccgctttc actgggatgt tcggggtggc gatcaagatc
     7081 gccctgccga tgatcggagt ctccttcctg ctttcgatcg cgatggggat tcttgccaag
     7141 acggcgcccc agctcaacat cctcatgctc ggttttccga tccagattct cgtcggattt
     7201 ctcgtgttgg tcgcctcgct gccgtttctc ttccggttta tgggaaacgt gatcagcgag
     7261 agtgtggcct tcgcggtcaa agtcatgggg ggcggctcat gatggaggaa agactgtttt
     7321 tggtgaacac cctcctgcct cctgagggga ggtttctcct cgatctgcag ttcttcgcag
     7381 ctgaagacga aggccggaca gaggatccga ccgaatcccg gaaacgcaag gcccgcgagg
     7441 aaggcaaggt cccgaaaagt caggacatgt ccgccgcgct cgtcctcctg gttgtttttt
     7501 ggacactggc gttttttggt tcccacatgg tgaccgggtt ccgggaggcc ttcacctact
     7561 gtatcgacaa tatgtatacc ctcgagatct cgacaaattc cctgcggacg ctcttcctgc
     7621 atttcgttct gttgtttttc gagatcctgg ccccgatcct gatcatggcc atcatcatcg
     7681 gggttgcagc gaatgtcatg caggtcgggt tccttttcac ctggaaaccg gtcaagccag
     7741 agctctcgag gatctcgttc actccccaaa aaatcttcca gcgtgtcctc ttttccaaac
     7801 aggccatggt caatcttgtc aaatccgttc tcaagattgt cgtaatcggc attgtcgcct
     7861 ggctgatcat tgaaggggag ttgcccaaga tgatcaatct gatggacgtc ggggtggccg
     7921 gcgccatgga ttccatcacc aatgtcgtgt ttgtcatggt caacgcgacc tgtctcgctc
     7981 ttttggcgct ctccgtaccg gattacatct tccagcgccg gcagcacctc gaatccttga
     8041 agatgtccat tcccgaaatc aaggaagaac acaagatgga ggagggagat cccctgatca
     8101 agagtcggat catgcaaaag agccgcgaac tcgcccagcg caatctcccg gccatggtca
     8161 aaaaggcgga tgtggtcatc acaaacccga cacacctctc cgttgccctc cagttcgaca
     8221 tgaacatcca tgaccagccg gtcgtggtgg ccaagggggc cgaccacctg gcctttgtca
     8281 tccggcagat cgcgaccgag gccggggttc ccatcatgga aaacaaaccg ttggcccggg
     8341 cgctctatca cgatgtcaat ctcggggacc cgatccccga gagcctcttc agtgccgttg
     8401 tcacggtcta tcaacggctc gagaaattca gaaaccggga ttattcgggg gtgcgcgcat
     8461 gatccatcag gaataccgtc tccggggact gtctccggta aaggaggttg tccatggctg
     8521 aaccaggccg caagggactc caggctgaca tcatgctggc ggtcgccgtg atcgccgtcg
     8581 tgttcatgtt gatcatcccg cttccgacga tgatgctgga catgctgatc gccgtgaacc
     8641 tgatgatctc cctgatcgtc ctcctgaccg tgatgtacat gaagaatgcg gcggattttt
     8701 ccatctttcc ctcgcttctt ctcttgacga ccgtcttccg tctggcactc aacgtctcga
     8761 cgacccgcgt gattctttcc gaggggatgc agttcgatgg agccatcatc cgcgcctttg
     8821 gagaattcgt tgtcgaaggg aattacgttg tc
//
LOCUS       DAOSHE010000032         8802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6787_length_8802_cov_4.146908, whole genome shotgun sequence.
ACCESSION   DAOSHE010000032 DAOSHE010000000
VERSION     DAOSHE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_8802_cov_4.146908"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLD82_01770"
     CDS             complement(<1..883)
                     /locus_tag="PLD82_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64147.1"
                     /translation="MPLARQRCLTPSFPPQARVLTMLDEAGTALVYGRSFDWPCFPED
                     DIGPLLERALPLDACLSPHELLAVAAWCASLGSLTRYHETAASCAVPRSLLDPLFAAI
                     TAHPDLVQAIRKAIDDQGEIKDSASRELNLIRHEIRDLSQRANRILQRMLNDSRLEGI
                     WQERLVSLRDGRHVVPVKAGQNGRLRGIILDRSTSGETLFVEPDAVVGIDNGIKELQI
                     AEDREIRKVLLDLTKRLRQHASAIGMTRNAAADLDCIFARARLADSMNGIRPEFVAHT
                     RPLEILEARHPLLTCTPV"
     gene            1066..2058
                     /locus_tag="PLD82_01775"
     CDS             1066..2058
                     /locus_tag="PLD82_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG64148.1"
                     /translation="MTHLVRTLGILCLVLCTCHLVVADDGGGEITASDSASLVRRAIG
                     AASHAVLERLLQRQPDLVNHIYEDGLSPLHVAVVQRNHAAVRLLLERRAEVNRRLQAA
                     AFHDREMEGVTALHLAVRANSPDIVRLLAAAGSDLNSRDTQGRTPFTLARTLDFREVA
                     YELRRHGAASFEDHLRAVLDGDVRAVEADLAAHPRYLARKDGNGQRPVDHAIRTGKAA
                     IVSAFLKAGLGPGDLDRHGNTLLHIAAWEGQARIIQILLEAGFPVDPETNGLRPIHIA
                     ASRGQTECVLMLLSAGADAHIEVEGRDPAAIARARGFVGTATAIEDFIRRKTRH"
     gene            complement(2033..3622)
                     /locus_tag="PLD82_01780"
     CDS             complement(2033..3622)
                     /locus_tag="PLD82_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG64149.1"
                     /translation="MTSDTKPSQDWAYSFDDFDHFRDLSHLSYMVVQKVLEHVQCERV
                     HFFIHNLSLREYREEDRENIFSITEDNPLITYLALQNRIIDRKNLRDDPIFREYDVNS
                     FILIDQLGVGTLVPFVYRYQLLGFLGITQQEQQTKLTREERSFLHALQRDLLQNLYAA
                     LMIDRRFSELVILSDLGKEISSIKDLPELFDVLFEKISRVFRFDTGVLWLAENEEMSD
                     LPVLGIAASKGLAPEWQTMRLVSGESISGHVYQAGKPLLVKELTRNAFFSQSNREPWL
                     TDALLSIPLRSSDHSIGVLTLHCSGDTTGFTGENLHLISIFANFTAATISNIRLYSKL
                     EKGYIETISALAAALDAKDPYTKGHSERVMHYAVGMATRMGLSKKKIRLIQFAAILHD
                     IGKIGISGDIIRKESGLTDDEYAIIKRHPEIGEQIISAIDFLQDARIFIKYHHERYDG
                     SGYYRMDKTDLPREAMILSIADSFDAMTTDRPYRRALSFEQALSELRSDIGRQFDPES
                     YDALVQFLRETGKISDGFSAG"
     gene            3789..4292
                     /locus_tag="PLD82_01785"
     CDS             3789..4292
                     /locus_tag="PLD82_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64150.1"
                     /translation="MVQQRIIAVLVCLLIAACSGETKPDDGNKGKNEPQGILRIAKFE
                     FRLEDTDPWSESALRTVLGSGERIRLRKEILFSNDDLVNAYLGYDEIFEATNLWLIFS
                     PEAGKRLKEITGKNVGKRLAIMVDGDVWIAPRILQEIPEGKASISGKRVEPYINRLGE
                     RLKQIVQ"
     gene            4414..4653
                     /locus_tag="PLD82_01790"
     CDS             4414..4653
                     /locus_tag="PLD82_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOG64151.1"
                     /translation="MASYVRIEPENCKGCGLCIVVCPTKQLSFSGKFNSHSYNYIQAS
                     NDNCRGCGFCYYTCPEPGAIEVHQDTGKKEQGGAQ"
     gene            4650..5696
                     /gene="vorB"
                     /locus_tag="PLD82_01795"
     CDS             4650..5696
                     /gene="vorB"
                     /locus_tag="PLD82_01795"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG64152.1"
                     /translation="MTRLVKGNEAVVMGSLLAGCQSYYGYPITPASEIAHTAARLFPQ
                     LGRTFLQAESEVAAINMVYGAACSGERTMTASSGPGISLKAEGISYLAGSELPCVIVD
                     VMRAGPGLGNIGPEQSDYNQVVKGGGHGNYRVIALAPNSAQEMCDMAMDAFDLADKYR
                     TPVVVLTDGVIGQMMEAVTFPKPREVGGVKPWAVSGTSETRSNLVTSIFLDFDELEAH
                     NRKLIAKYEAIEAAEQRWQGYLLDDAEVVIVAYGVTSRESRSVCERLRAVGIRAGLFR
                     PQTLWPFPKDGLREVASRVDKIFVAELSMGMLVDDVRLTLNDGRVPGFAANYGGNLLK
                     GDTLFAAVRSFVGK"
     gene            5701..7140
                     /locus_tag="PLD82_01800"
     CDS             5701..7140
                     /locus_tag="PLD82_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG64153.1"
                     /translation="MEKIIKKPSTMYDRFERKPGSDKENTHYCPGCGHGIIHKIIAET
                     LEELGIADRTVLISPVGCSVFAYYYFDCGNIQVAHGRAPAVATGVTRSNPDNFLISYQ
                     GDGDLAAIGTAEIIHAANRGEKMTVFFVNNSIYGMTGGQMAPTTLIGQKTTTTPFGRN
                     ALNEGNPMKMAEMISILDAPVFVARTSVHDIKHIMQTRKAVKKALQAQKDGKGFALVE
                     ILSPCPSGWKINPQQNAEWVEKHVLSVFALGEFKDEISQREGVSRARKAASPEEVLAV
                     LGLDSEESVFAPQNDSFSRMGVVFAGFGGQGIMMLGQMLSLLGMRHGLAVTWLPSYGP
                     EMRGGTANCSVVLSTDPIGSPVVDAPELLVAMNGPSLEKFLPRVAPGGLLLYNSSIIE
                     QKPERQDISVLGVPAADMARAAGTERAANVVMLGVLAGWYGSFGDAALTSVLEEFFPK
                     KDLLLLNKKALEAGMVFGAQARADQKGMP"
     gene            complement(7262..7335)
                     /locus_tag="PLD82_01805"
     tRNA            complement(7262..7335)
                     /locus_tag="PLD82_01805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7299..7301),aa:Arg,seq:cct)
     gene            7487..8242
                     /locus_tag="PLD82_01810"
     CDS             7487..8242
                     /locus_tag="PLD82_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG64154.1"
                     /translation="MRLQTTTLWDYPSQHYGTGMQGDKEYIGATPSWVIWNLLKRYTK
                     RKDLVVDPMCGSGTTIDVARDLERRALGYDLAPSRKDIFRADARKIPLEDEKADFVFV
                     DPPYSDHIRYSGKPECIGELSAEGDAYYLAMEQVISEINRILRPGRYMALYVSDSWEK
                     GKPFMPIGFYLLQMLSQHFGIVDIIAVIRHNGKLMRNHWHTAAVEHNYYLRGFNYLFI
                     MYKPAAGERVPQDRREADEIAEEIEARAVHKDG"
     gene            8247..>8802
                     /locus_tag="PLD82_01815"
     CDS             8247..>8802
                     /locus_tag="PLD82_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOG64155.1"
                     /translation="METETVEIPQGFRLLALEGGIKYAGRKDIGLIISTRPCSYAGAF
                     TTNRVQAAPVRLCRERLGQSIHAVIVNSGSANACTGDEGYRDAVTITRHVEKGFGLPK
                     GSALSLSTGVIGVPLPVAKFEAALADTSHAETGPEAFARSIMTTDTVPKMAARTVALP
                     GGRSVKIAGFAKGAGMISPNMATML"
BASE COUNT         1899 a         2338 c         2644 g         1921 t
ORIGIN      
        1 gtaccggagt gcaggtcagg agagggtggc gtgcttccag aatctcgagt ggccgggtat
       61 gggcgacgaa ttccggccgg ataccgttca tggaatctgc cagtctggcc cgggcaaata
      121 tgcagtcaag atcggccgct gcatttcggg tcatcccgat tgcggatgcg tgttgccgca
      181 gccgtttggt cagatccagg agcaccttcc ggatttcgcg gtcttcagca atctgcaatt
      241 ccttgatgcc gttgtcgatt ccgacgacgg catccggttc cacgaacagg gtttccccgg
      301 aggttgagcg gtccaggatg attcccctga gacggccgtt ttggcctgct ttgacgggca
      361 cgacatggcg cccgtcacgg agcgagacaa ggcgttcctg ccagatccct tccagtcggg
      421 agtcattcag cattcgctgg aggattcggt tggcgcgttg ggagaggtca cggatctcgt
      481 gtctgatgag attcaattcg cgcgaggcac tgtccttgat ctctccctgg tcatcgatgg
      541 ccttgcggat ggcctggacc agatccgggt gtgccgtgat ggcggcgaag aggggatcaa
      601 gcagggaacg ggggaccgca caactggcag ccgtttcgtg atagcgtgtg aggctgccca
      661 gacttgcgca ccaggcagca acggccagga gttcgtgggg agagaggcat gcgtcaaggg
      721 gaagcgctct ttcgagaagg gggccgatgt cgtcttccgg aaagcacggc cagtcgaagg
      781 aacgaccgta taccaaagcc gtgccggctt catcgagcat tgtcaatacc cgggcttgcg
      841 ggggaaagga gggcgtcagg cagcgctgcc tggccagggg catccgtgca aagccggcga
      901 gtttctcgag gatccgggtg tagcccagag cggtcagggt tcgttgatcc agatgatcct
      961 cccggacaaa gtgtgtctcc ttctaaaggt acacagaggg cccgtgacat tctgccagtt
     1021 ttttcccggg atgttatatt tgaaaaaagg aattatgggg ggagcatgac gcatcttgtc
     1081 aggacactcg gtatcctgtg cctggtactc tgtacctgcc acttggtggt cgccgatgat
     1141 ggcggtggag agattaccgc ctccgacagt gccagtctgg tccgccgggc aatcggcgcc
     1201 gcttcccatg ctgttctgga gcggcttctc cagagacagc cggatcttgt caaccacatc
     1261 tatgaagacg ggctttctcc tcttcatgta gccgttgtac agcgcaatca tgcagcggtc
     1321 agactcttgc tggaacgcag ggccgaggtc aaccgccgcc ttcaagccgc agcctttcac
     1381 gaccgtgaaa tggaaggtgt gaccgccctg cacctggcag tccgggcgaa ttctccggac
     1441 atcgtccgcc tcctggccgc tgccggctcg gatttgaact caagggacac ccagggccgg
     1501 accccgttca ccctggcccg cacgctggat ttccgcgagg tcgcgtacga actgagacgg
     1561 cacggggctg cgtccttcga ggaccatctc cgggccgttc ttgacggaga tgtacgggcg
     1621 gtcgaggcgg atcttgcagc ccatccacgc tatctcgcga ggaaggacgg caatggccag
     1681 aggcccgtcg atcacgccat ccggacaggg aaagcggcga tcgtgtcggc attcctcaag
     1741 gccggcctcg gtccgggaga tcttgacaga cacgggaaca cgcttctgca cattgctgca
     1801 tgggaaggcc aggcccggat catccagatc cttctcgagg ccggctttcc ggtcgatccg
     1861 gagaccaatg gtctgcgccc gattcatatt gcggcttcca gaggtcagac ggagtgtgtg
     1921 ctgatgcttc tctcggccgg agccgatgcc catatcgagg ttgaaggcag ggatccggct
     1981 gccattgcca gagcgagagg gtttgtgggg actgcaaccg caattgagga tttcatccgg
     2041 cggaaaaccc gtcactgatt tttcctgttt cccggagaaa ctggacaagc gcgtcatagg
     2101 attcgggatc gaactgtcgc ccgatatcgg agcgcagttc cgaaagggct tgttcaaagc
     2161 tgagggctcg ccggtacggt cggtctgttg tcatggcatc gaatgagtcc gcgatgctca
     2221 ggatcatggc ttcgcgcggg agatcggtct tgtccatccg gtaatagccg gagccgtcat
     2281 accgttcgtg atggtacttg atgaaaatcc tggcgtcctg gaggaagtca atggccgata
     2341 ttatttgttc tccgatttca ggatggcgct tgatgatggc atactcgtcg tcggtcaatc
     2401 cggattcttt ccggatgatg tccccactga tccctatttt cccgatatca tgcaggatgg
     2461 cggcaaattg tatcagacgg attttctttt tggacaggcc catccgggtc gccattccga
     2521 cggcgtagtg catcacccgt tccgaatggc ctttggtgta gggatccttg gcatccagag
     2581 ctgctgccag ggcgctgatc gtctcgatgt agcctttttc cagcttgctg tacagccgga
     2641 tgtttgaaat tgtggcagcg gtaaaattgg cgaagatcga gatgagatgg agattctcgc
     2701 cggtaaaacc tgtggtgtca cctgaacaat gcagggtcag aacccctatc gagtggtcgc
     2761 tcgagcggag gggaatcgag agcagggcgt cggtcagcca aggttcccgg ttggattggc
     2821 tgaaaaaagc gttccgggtc agctccttga ccagaagcgg cttccccgcc tgatagacgt
     2881 gtcccgaaat ggattccccg ctgacaagcc gcatggtctg ccattccggg gccaacccct
     2941 tgctcgccgc aatcccgagc acgggaagat cggacatttc ctcattttcg gccagccaga
     3001 gcactccggt atcaaagcgg aagacccggg atatcttttc aaacaggaca tcgaagagtt
     3061 cagggagatc cttgatgctc gagatctctt ttccgaggtc ggaaagaatg accagctccg
     3121 aaaatctccg gtcgatcatc agggcggcat acaggttctg caggagatcc cgctggagtg
     3181 catgcagaaa actccgttcc tccctggtca attttgtctg ttgctcctgc tgtgtgattc
     3241 ccaaaaaccc caaaagctga taccggtaga caaacgggac cagggtcccg acgccaagct
     3301 ggtcaatcag gatgaaggaa ttgacatcat actcccgaaa aatgggatca tcccgcaggt
     3361 ttttcctgtc aatgatcctg ttttgcaggg cgagataggt gatcagggga ttgtcttcgg
     3421 ttatggagaa aatattttca cggtcctcct cacgatactc tctcagactg aggttgtgaa
     3481 tgaagaaatg cacacgttcg cattggacgt gctcaagaac cttctggaca accatgtacg
     3541 aaagatgcga gagatcccga aaatggtcaa agtcgtcgaa ggagtatgcc cagtcctggg
     3601 agggttttgt atcgctcgtc aatgatgatc gcctgcccat atcaaatatt tttgatattg
     3661 tatgccgcat tgttttttgg tgcaatgaaa aaagacaggc tccgtcttgc gttgaggatt
     3721 gaaaaatccg atggatatga tagaatccca agtacacgaa tgtgatgtcc gggctgagga
     3781 gccactgaat ggtacaacaa agaatcatcg ctgtgcttgt ctgtcttttg attgctgcct
     3841 gttcaggcga gacgaagccg gatgacggca acaaaggcaa aaacgaacca caggggatcc
     3901 tgaggatcgc caaattcgag tttcgcctgg aggacacgga tccctggtcg gagagtgcct
     3961 tgaggacagt tctgggaagc ggtgaacgca tccgtctgcg caaggagatt cttttcagca
     4021 atgatgatct ggtcaatgcc tacctgggat atgacgaaat cttcgaagct accaatctct
     4081 ggctgatttt ctccccggaa gccggaaagc ggctcaagga gatcaccggg aaaaacgtcg
     4141 gaaagcgact ggccatcatg gtcgatgggg atgtctggat tgcgccacgg atcctgcagg
     4201 agattcccga gggaaaggcc tccatatccg gaaagcgtgt cgaaccatac atcaatcggc
     4261 tcggagagcg gctcaaacag atcgtccagt aacccgtcct gtccgctcag gatgaagcca
     4321 tcgcccatcg ggtggtggct tcttgctttt gccttgactc cgtgagtatg ttcattccaa
     4381 gaaactcggt gtgtttcagg aaaggataga gccttggcgt cctacgtccg tatcgaaccc
     4441 gaaaactgca aggggtgcgg cctgtgcatc gtggtctgcc cgaccaagca gctttcattt
     4501 tcaggcaagt tcaattccca cagttacaat tacatccagg catccaacga caattgcaga
     4561 gggtgcggtt tctgttatta cacatgccct gagccggggg cgatcgaggt tcaccaggat
     4621 accggcaaaa aagaacaggg gggtgcacaa tgacccgcct cgtcaagggc aatgaagccg
     4681 tcgtgatggg atctcttctt gccggctgtc agtcctatta cgggtatccc attactccag
     4741 ccagtgagat agcccacaca gcagcccgct tgttcccgca gctcgggaga acgtttctcc
     4801 aggccgagag tgaagtggca gcaatcaata tggtctatgg tgcggcctgt tccggagagc
     4861 ggacgatgac ggcctcctcg ggacccggga tcagcctcaa ggcagagggc atatcgtacc
     4921 tcgccggctc ggagcttccc tgcgtgattg tggacgtcat gcgcgccggc cccggcttgg
     4981 ggaatatcgg cccggagcag agcgattata accaggtggt caagggaggg ggacacggga
     5041 attaccgtgt catcgccctg gccccgaact ccgcccagga gatgtgtgac atggcgatgg
     5101 acgccttcga tctggcagac aaataccgga caccggttgt tgtgctgacg gacggcgtga
     5161 tcgggcagat gatggaagct gtcacctttc cgaagccacg tgaagtcggg ggcgtgaagc
     5221 cttgggctgt ttcaggaact tccgaaaccc ggtcaaacct tgtgacatcc attttcctcg
     5281 atttcgacga gcttgaggcg cacaaccgca agttgattgc caagtatgaa gccatcgaag
     5341 ccgcagaaca gcgttggcag gggtatctcc tggacgatgc cgaagtggtg attgtggcct
     5401 atggtgtgac cagccgtgaa tcgcgctcag tgtgcgaacg cctgcgggcc gtgggtatcc
     5461 gtgccggcct cttcaggccc cagacactct ggccatttcc caaggatggc ttgcgtgagg
     5521 tcgcatcccg ggtcgacaag atctttgttg cggaactctc catgggaatg cttgtggacg
     5581 atgtccgact gaccttgaat gacggacggg tgccaggatt tgcggcaaat tacggtggga
     5641 atcttctcaa gggcgatact ctatttgctg ctgtgcgcag cttcgtgggg aaatgacgcg
     5701 atggaaaaaa tcataaaaaa accgtcaaca atgtacgaca ggtttgagcg gaaacccggg
     5761 tcggacaagg aaaatacgca ttactgtccg gggtgcgggc acgggatcat ccataaaatt
     5821 attgccgaga cactggaaga actgggaatc gccgaccgga cagtcctgat ttcaccggtc
     5881 gggtgcagtg tctttgccta ttactacttc gattgcggca atatccaggt ggcccatggt
     5941 cgggctccgg ctgtcgcgac cggagtgact cgatcgaatc ctgacaattt tctgatttcc
     6001 tatcaggggg atggcgatct ggccgcgatc gggactgccg agatcattca cgccgccaac
     6061 cgtggcgaga agatgacggt cttttttgtc aacaactcca tctacgggat gacaggtggc
     6121 cagatggcac cgacaacctt gatcggccag aagaccacga cgaccccgtt cggccggaat
     6181 gccctcaatg aggggaatcc gatgaaaatg gctgaaatga tttccatact cgatgcgcct
     6241 gtctttgtgg cccggacctc ggttcacgat atcaagcaca tcatgcagac cagaaaagcg
     6301 gtcaaaaaag cccttcaggc ccaaaaggac gggaaggggt ttgcgctggt tgaaatcctg
     6361 tccccctgtc cctcgggctg gaagatcaat ccccagcaga atgccgaatg ggtcgagaaa
     6421 catgtcttgt cagtgttcgc cttgggtgag ttcaaggacg agatctcgca gcgtgagggt
     6481 gtttcccggg cccgcaaggc ggccagtccc gaagaagtcc ttgccgtgtt gggactggac
     6541 agcgaggaga gcgtctttgc tccccaaaat gacagctttt cccgcatggg cgtcgtcttt
     6601 gccgggtttg gtgggcaggg catcatgatg ctcggccaga tgctctccct gctcgggatg
     6661 cggcacgggc ttgcagtgac ctggcttcct tcctacgggc ccgagatgcg tggagggacg
     6721 gcaaattgtt ccgtcgttct ctccaccgat ccgatcgggt cgcccgttgt ggatgctccc
     6781 gaacttttgg tggccatgaa tggaccatcc ctcgagaagt tcctgccacg ggttgcaccg
     6841 ggcggcttgc tcctctacaa ctcttcgatc atcgagcaga agcccgaacg ccaggatatt
     6901 tccgttcttg gtgttcctgc tgccgacatg gcccgggctg ccggcacgga gcgggccgcc
     6961 aatgttgtca tgcttggggt gcttgccggg tggtacggaa gctttggcga tgcagctttg
     7021 acctcggttc ttgaagagtt tttcccgaaa aaggatctct tgctcctgaa caaaaaggcg
     7081 cttgaggccg gcatggtctt cggggcgcaa gccagggcag accaaaaggg gatgccgtga
     7141 accaggcatc tccctggttt ggatgatggt gttttttgat gaacaaaaag ccgcccctgt
     7201 atgggacggc tttttcgagt gggtgtgccg gatgaatcgg tgatgcaatc cggagagtgc
     7261 ttggccctgg cgcgactcga acgcacggca cgcggtttag gaaaccgctg ctctatccac
     7321 ctgagctaca gggccttgta cagacattcc cgcatggccg gttgtgcccg gacgcaaggg
     7381 gtcccaaaaa aatagcatgg accggctccc ttcggcaaac attttacagt ccttcttgct
     7441 tgcctgtgag gcttttgcat agatttgcag catgaagaaa ccgcccatga gactgcagac
     7501 gaccaccctc tgggattatc catcccagca ttatggtacc gggatgcagg gagacaagga
     7561 atacatcgga gccactccct cctgggtgat ctggaatctt ctcaaacggt acacaaagcg
     7621 caaggatctt gttgtggatc cgatgtgcgg gagtggcacg accatcgatg ttgcccgtga
     7681 tctggaacgg cgggcactcg ggtatgatct ggcaccctcg cgaaaagaca tctttcgggc
     7741 tgacgccaga aagattcctc tcgaggatga aaaggccgat ttcgtttttg tcgatccgcc
     7801 ctattcggac catatccgct actcgggcaa acccgaatgc atcggagaac tctcggccga
     7861 gggagatgcc tactatctgg ccatggagca ggtcatctcg gagatcaacc gcattcttcg
     7921 acccggacgg tatatggcgc tctacgtgag cgattcctgg gagaagggaa agcccttcat
     7981 gccgatcggg ttctaccttc tccagatgtt gagtcaacat ttcgggatcg tggacattat
     8041 tgcggtgata cggcataacg gaaagctgat gcggaaccac tggcatacgg cagctgttga
     8101 gcacaattac tatttgcgcg ggttcaacta tcttttcatc atgtacaagc cggctgcggg
     8161 cgaacgggtt ccacaggacc gacgtgaggc cgacgagata gccgaagaga tcgaagcccg
     8221 ggcagtacac aaggatggat aagagcatgg aaacggaaac tgtcgagatc ccgcagggct
     8281 tcagactgct tgcactcgaa ggtggcatca agtatgccgg acgaaaagac atcggtctga
     8341 tcatcagtac acgaccatgc agctacgccg gtgcgtttac aacaaacagg gtccaggccg
     8401 cgcctgtcag actctgcagg gaacgactgg ggcagagcat ccatgccgtc atcgtgaaca
     8461 gcgggagtgc caatgcctgt accggggatg aagggtacag ggatgccgtc accatcacga
     8521 gacatgtcga aaaagggttc ggtttgccga agggaagcgc gctctctctt tcgacgggtg
     8581 tcatcggagt gccgcttccg gttgccaagt tcgaagcggc actggcggat acctcgcatg
     8641 ccgaaaccgg tcccgaagct tttgcgcgat ccatcatgac gaccgatacg gttcccaaga
     8701 tggcggccag gacggtggcc ctgcccggcg gaagatccgt caagatcgcc gggtttgcca
     8761 agggtgcggg catgatctct ccgaacatgg cgaccatgct gg
//
LOCUS       DAOSHE010000033         8623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_6970_length_8623_cov_4.049253, whole genome shotgun sequence.
ACCESSION   DAOSHE010000033 DAOSHE010000000
VERSION     DAOSHE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_8623_cov_4.049253"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLD82_01820"
     CDS             complement(<1..1387)
                     /locus_tag="PLD82_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG64156.1"
                     /translation="MSFVHSMGLNPQQLVGATHRDGPLLILAGAGSGKTKVITTRIAW
                     LIAEQHVSGRSILALTFTNKAAREMKERVFGLLPKKSGKGLTVGTFHAFCVQLLRSKI
                     GLLGYREKFSIFDSSDRMHLITDIMKDLKIDLEILPPDKVAWKISDAKNALIHPGREA
                     EEWFGDALLASIYRHYQSTLKGFNIVDFDDLLMLAVDLFAVAGEEMALHVSRWRYLLV
                     DEYQDTNQAQYKLLRCLTRDHDNICVVGDDDQSIYAWRGADISNILNFEKDFPGTVVI
                     RLEQNYRSTGNILRAANSVIAHNTARKGKNLWTSGVNGDPVRIIPTSDETDEAESIVS
                     EILTIRREDRASFADMAIVFRTNFQTRPFEELFAAQGIPYEVIGGTKFFDRREVKDIV
                     CYLRLMANRHDEIALLRIINRPKRGIGTQTIEKIVQWAEKHTMHLYDVMLDADHVDTM
                     DDNTRYKIGVFC"
     gene            complement(1473..2324)
                     /locus_tag="PLD82_01825"
     CDS             complement(1473..2324)
                     /locus_tag="PLD82_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64157.1"
                     /translation="MGGLDDEAAKQEKIRNKARFNESIKKYKGDIDVIDKRIKLMEKD
                     IRTKKDALNGYRHVIIANEYLKIIGLYCAMNDESVQIMNFKNENYLNEARKLIYQVLI
                     HLEDVVTPHIDIPPNDLEDKLEAIAKLNPQRKLNLLNAIGFRIQQVVDAFGDNTKWKW
                     SFVEIDGRYAVVTKNMIDYRVAIANNDPRKPYYVENLNLMRMVKEQLEKASRRYREKY
                     EQTTKETEDIKKSIAFIAAMRRILILMGQSEEANKYKRIMELATNLMEENIKKKEAEK
                     RAPAKKK"
     gene            complement(2471..3163)
                     /locus_tag="PLD82_01830"
     CDS             complement(2471..3163)
                     /locus_tag="PLD82_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64158.1"
                     /translation="MKTILSLVLLVCLLLVPVNSAKAALPAPEDVYTRFVRNMATSRP
                     ASFSCIVGGDAVLRSLGRIPADARQGRPVVRTWYRRDVGQVIRVENVDPSFKNLFSAY
                     QSYLGMTGAWLAARGRDWREFSAQYSMQILGSSQQSILAKIATRGRENDSWGEFTFSA
                     HDMSVQSVRFFSRNSLIYTVQNLYGRIGGYLLPTSMVITAWNNGTTSSVSRLVFSEYR
                     LNPVVPDAVFSR"
     gene            complement(3205..3912)
                     /locus_tag="PLD82_01835"
     CDS             complement(3205..3912)
                     /locus_tag="PLD82_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG64159.1"
                     /translation="MDTMRDVIARLVELQSVLKEIYDIEQMNKRLPEGVEQMKRNLEP
                     LKREIETSEAELCDVETRQGQLAVESQELQDRLQEISRRMTTISKTRELEAVELEFSS
                     ANERISDCNRQIKETRSRHDALVKRLEEMRPEYDQNLEKIVAEETKVQDEVAKNQLQI
                     DELRARRQTMVVGVDMPTLNRFEKIVNSKNGIGIVPVINDSCDGCHMRIPPQMIGKVK
                     RCQELVTCLNCSRILYL"
     gene            complement(4082..4154)
                     /locus_tag="PLD82_01840"
     tRNA            complement(4082..4154)
                     /locus_tag="PLD82_01840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4119..4121),aa:Ile,seq:cat)
     gene            complement(4213..6060)
                     /gene="rpoD"
                     /locus_tag="PLD82_01845"
     CDS             complement(4213..6060)
                     /gene="rpoD"
                     /locus_tag="PLD82_01845"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOG64160.1"
                     /translation="MLDHNEARVLQRLIELGRKSGGLTYDMINDELPDSLGNPEKMDE
                     LLTSLGDAGVTVVDVREIGGAAPVEQSTHDEVHEDDADTHLEDEEQHDQMEDADEHPA
                     VIEQDVHAVAGKLYRHDDGPASDDPIRHYLRDIGKVSLLSAEQEVVLAKRIEQGEQEI
                     EEVVVHSYAVIPEIKRIIDRITARKASLHDLIETGRVINLTQEEKTSYEERFEILSKE
                     ILKIEKALKKLGVDSEPGDDEDSGDESRTEVQKLINRFSARLRTVRLSRHVVREVAEK
                     LISAGNRIEEQQKVFSEMEKRFGFALTSVRGLRKYLDKGDANGLNTYCTKHGIPADEA
                     ESIHKEYRNGQRRLRRIQMECNAGLDEILETAGRLQAAELQISKAKESLVQANLRLVV
                     SIAKKYVNRGMHFFDLVQEGNIGLMKAVEKFEYQKGYKFSTYATWWIRQAITRSVSDQ
                     ARTIRVPVHMIEQINKVLRESRVLLQQMGREPSPQEIADSLGWAVGRVKNILSVAKEP
                     ISLETPIGEDDDSYLYDFIEDKDADNPVNQTANRLLREHLDAVLRTLPKREQQVLRMR
                     FGLDDGYNHTLEEVGYKFNVTRERIRQIEAKALRRLRHPTRIRILRDFI"
     gene            complement(6060..7913)
                     /gene="dnaG"
                     /locus_tag="PLD82_01850"
     CDS             complement(6060..7913)
                     /gene="dnaG"
                     /locus_tag="PLD82_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG64161.1"
                     /translation="MVSPEVIERIREALPLVEFIGRDVALKRAGSIYKGLCPFHNERT
                     PSFAVYPEKNRYYCFGCGKKGDVFDYVQERNHLGFGEALVFLADASGIELPSDNPAEN
                     AQAAIHRRWMDLYEKATTMYRHALKVLPAGKSALAYLEGRGVRAETIDDFRLGYALPQ
                     WDALSSKLLREQYEAETLVECGLSRASREGRRIYDYFRDRVMFPIADERGRVVAFGGR
                     VLDGSEPKYLNSPESPVFKKREILYGLDRARTAIGDKDLAIIVEGYLDVIGLHQAGFS
                     NVVAPLGTALTSWHLNRLKKLTSHIIFLFDGDKAGRRAAFAAARPAMEAGCLARVVLL
                     PSELDAFDISLKHTREQIEALLRQSIPLADFVINTIYQGSEVDSGDRPMMFLQGVYQF
                     LEGMEDGVQTSLFLQKASLLAGVDSDEVELDFRRQRQKPVMTTAKKMGPAPAPVAKFG
                     QSPAGQDGDFELYLMRLAALHSSAWPDINKEIGKGLRIEDSRARSLLEIISSIAGDKT
                     VWSAADVLARVSPGEIRSILEEDMASGRLEIDWQKQFGDTIDTLWIKTIRRERALLDD
                     EMRRQKLLGDDNAIQTLQQEILVLRRREDDLARRMRTVRRETGNKTEGGQG"
     gene            complement(8069..8281)
                     /gene="rpsU"
                     /locus_tag="PLD82_01855"
     CDS             complement(8069..8281)
                     /gene="rpsU"
                     /locus_tag="PLD82_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG64162.1"
                     /translation="MLGIRVKDSEPIELALKRFKKECEKAGLLTEIKKREHFEKPSMI
                     RKKKDAAARRKREKKMKIARAKAKHV"
     gene            complement(8365..>8623)
                     /locus_tag="PLD82_01860"
     CDS             complement(8365..>8623)
                     /locus_tag="PLD82_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOG64163.1"
                     /translation="FRDIQPQAPMHILIIPRDHVASLDAVQDPVLIGYIMTTAAKLAR
                     EHGVAEGGYRVVVNCNQDGGQTVFHLHAHLLGGRFMTWPPG"
BASE COUNT         1840 a         2475 c         2194 g         2114 t
ORIGIN      
        1 cgcaaaaaac cccgatcttg taccgggtat tgtcgtccat ggtatccaca tggtccgcat
       61 caagcatgac gtcatacaga tgcattgtgt gtttttcggc ccactggacg atcttttcaa
      121 tcgtctgggt cccgattccc cgcttgggcc ggttgatgat ccgcaggagg gcaatctcgt
      181 cgtgcctgtt ggccatcagt ctcagatagc agacaatatc cttgacctca cgcctgtcga
      241 aaaattttgt cccgccgatc acttcgtacg gaatcccctg ggcggcaaac aattcttcaa
      301 atggtctcgt ctggaaattg gtccggaaaa caatcgccat gtccgcgaag gatgcccggt
      361 cctcacgccg gatggtcaat atttccgaca cgatcgactc ggcctcgtca gtctcgtcac
      421 tcgtcggaat gatccggacc gggtctccat tgacaccact cgtccagagg tttttgccct
      481 tcctggccgt gttgtgtgca atcacggagt tcgcagcccg gagaatattc cccgttgatc
      541 gataattctg ttccaggcgg atgacaacag ttccgggaaa gtctttttca aaattcaaaa
      601 tattgctgat atccgcccca cgccatgcat agatggattg gtcatcatcc cccacgacac
      661 agatattgtc atggtctctg gtcagacatc gcagaagttt atactgggcc tgattggtgt
      721 cctggtactc gtcaaccaga agataccgcc agcgcgaaac atggagcgcc atctcctccc
      781 cggcaacggc aaaaagatcg actgccagca tcaggaggtc gtcaaaatcg acaatattga
      841 agcccttgag cgtcgattgg tagtgccggt agatgctggc gagaagggca tcaccgaacc
      901 actcctcggc ctcccgcccg ggatggatca gggcgttttt ggcatccgaa atcttccatg
      961 ctaccttgtc cggggggagg atctccagat cgattttgag atccttcatg atatcggtga
     1021 tgagatgcat ccggtccgat gaatcgaaga tcgaaaactt ttccctgtat cccaacaaac
     1081 cgatcttgct ccgcaacaac tggacacaaa aggcatggaa tgttccgacg gtcaaacctt
     1141 tgccggattt tttcggcagg agtccaaaga cccgctcctt catctctcgg gcggccttgt
     1201 tggtaaatgt cagggcaaga attgaccgcc ctgaaacatg ctgttcggcg atcaaccagg
     1261 caatcctggt tgtgatgacc ttggtcttgc cgctcccggc tccggcaaga atcaggagcg
     1321 ggccgtcacg atgggtggct ccgacaagtt gctggggatt gagtcccatt gaatgaacga
     1381 atgacacatt tccctccgcc agtccataat agcgaaggaa gatgcgttcc ggcaatcttc
     1441 gtcataccag aagagatgcc cgttattcgg ggttatttct ttttggcagg agcccgcttt
     1501 tcggcttctt tctttttgat gttttcttcc atcagattgg ttgccaactc catgatccgt
     1561 ttatacttgt ttgcttcctc gctctgtccc atcaggatca gaatccggcg catcgccgct
     1621 atgaacgcaa tggatttttt gatatcttcg gtttccttgg tcgtctgctc gtacttctcc
     1681 cgataccgcc ggctggcttt ctccagctgt tccttgacca tccgcatcag attcaggttt
     1741 tcaacgtagt atggcttgcg cggatcattg ttggcaatcg cgacccggta atcgatcatg
     1801 ttttttgtca cgacggcata gcgaccgtca atctcgacaa agctccattt ccatttcgta
     1861 ttgtcgccaa aagcatcgac aacctgctgg attctgaacc cgatggcgtt caggagattg
     1921 agcttgcgtt gcggattgag ctttgcgatg gcctccagct tgtcttcaag atcattggga
     1981 ggaatatcga tatgcggagt cacaacatct tccaggtgga tcaggacctg atagatcagc
     2041 ttgcgcgctt cattcagata gttttcattc ttgaaattca tgatctggac agattcgtcg
     2101 ttcatcgcac aatacaggcc gatgatcttg aggtattcat tggcgatgat gacatgccgg
     2161 tacccattca gggcatcttt ttttgtcctg atgtctttct ccatcagctt gatgcgcttg
     2221 tcgatgacat cgatatcgcc tttgtatttc tttatacttt cgttgaatcg tgctttgttg
     2281 cggatcttct cctgctttgc agcttcgtca tccagaccac ccacagactt tcctcctgcc
     2341 ggttacaaga cctgccacct ccttcttcta tcggcattga tccgtgcagg atgaagcatc
     2401 gaaattccgg gaccggcact ggccggcccc ggaaaaagcc aaaaaagagc cggagaatca
     2461 agcagccgga tcagcgcgaa aaaacggcat caggaacaac gggattgagc cggtactccg
     2521 aaaaaacgag ccggctgaca ctggaggtgg taccgttgtt ccaggcagtg atcaccattg
     2581 acgtgggcaa gaggtatcca ccgatccttc cgtacagatt ctgtacggta tagatgaggg
     2641 agttccgtga aaaaaagcgg accgactgga cagacatgtc gtgtgcactg aacgtgaact
     2701 cgccccaaga atcgttttcc cgtccccttg tagcgatttt ggcaagaatc gactgctggc
     2761 ttgatccgag aatctgcata ctgtactggg ctgaaaactc gcgccaatcc cttccgcggg
     2821 cagccagcca ggcgccggtc attccgagat agctctggta cgccgaaaaa agatttttga
     2881 aggacggatc gacattttcc acccggatga cctggccaac gtcgcgtcga taccaggtcc
     2941 ggacgaccgg tcggccctgg cgggcatcgg cgggaatcct gcccagactg cggaggacgg
     3001 catcaccccc gacaatgcag gagaaactcg ccggccggga tgtcgccata ttgcggacaa
     3061 accgggtgta tacatcttcg ggagcaggga gtgcggcttt ggcagaattg acagggacaa
     3121 gcagcaagca caccagcagt acaagagaaa ggattgtctt catgcggaca agtgacacca
     3181 cagccgtcgg atcgtcaaga agtcttacaa gtacaggatg cgggaacagt tcaggcaggt
     3241 tacaagctcc tggcatcgtt tgacttttcc tatcatctgc ggaggaatcc tcatatgaca
     3301 accgtcgcaa ctgtcgttga tgaccgggac aatcccgatc ccgtttttgc tgttgacgat
     3361 cttctcgaaa cggttgagcg tcggcatatc gaccccgaca accatggtct gtcgccgggc
     3421 acgaagctcg tcaatttgca attgattttt tgccacctcg tcctggactt tggtctcttc
     3481 agcaacgatc ttctcgagat tctggtcata ttctggtctc atctcttcga gtcttttgac
     3541 aagggcatcg tgccgggaac gggtctcctt gatctgccgg ttgcagtcgg atatcctctc
     3601 gttggctgat gaaaactcga gttctacggc ctcaagctcc ctggtcttgc tgatcgtggt
     3661 catccggcgc gagatctcct gcagtctgtc ctgcaactcc tgtgactcga cagcaagttg
     3721 cccttggcgg gtctcgacgt cacaaagctc ggcctcggac gtctcgatct cccgcttgag
     3781 gggttcgaga tttcttttca tctgctcgac accttcgggc agacgcttat tcatctgctc
     3841 gatgtcatat atttccttga gaacagactg aagctcgaca agtctggcaa tcacatcccg
     3901 catcgtgtcc atactgaccc ctttttccgc gctgaacggt ctctcaccct cacgggtgaa
     3961 cgtatctgaa ctgacagaaa aaaaagcata aaggtctcaa cccctttatg ctttgaaacc
     4021 gtggacagca tgctgtgaac aaggacctgt ctgcatcatg cccgccacct ttaccgtaat
     4081 ctgggcccac ctggacttga accagggacc atcgagttat gagcacgccg ctctgaccac
     4141 tgagctatgg gcccgtgccc ggtaaaggta ttcccggaaa aattcacggt caagaaaaac
     4201 tctcgcggct tgtcagataa aatcacgcag aatccggatt cttgtcgggt gtcgcagtct
     4261 gcgcaaggcc ttggcctcga tctgccggat tcgctccctg gtgacattga atttgtaccc
     4321 gacctcttcg agggtatggt tgtagccgtc atcgaggcca aaacgcatgc ggagcacctg
     4381 ctgttcccgc ttgggcaggg tccggagcac ggcatccaga tgctcgcgca gaagacggtt
     4441 ggccgtctgg ttgacaggat tgtccgcatc cttgtcctcg ataaaatcat acagatagga
     4501 atcgtcatcc tcgccgatcg gtgtctcgag agaaatcggt tccttggcga cactcaggat
     4561 gttttttacc ctgccgacgg cccacccgag gctgtccgca atctcctgcg gcgatggttc
     4621 acgccccatc tgctggagga gaacccgaga ctcgcgcaag actttgttga tctgttcaat
     4681 catgtggacc ggaacccgga tggtgcgggc ctggtctgaa accgatcggg taatggcttg
     4741 ccggatccac caggtggcat aggtcgagaa cttgtaccct ttttgatact cgaatttctc
     4801 gacggccttc atcagcccga tattcccttc ctggacaagg tcgaaaaagt gcattccacg
     4861 gttgacatat tttttggcga tgctcaccac cagcctgaga ttggcctgga caaggctctc
     4921 cttggctttc gagatctgga gttccgccgc ctgcaatcgt cccgctgtct cgagtatctc
     4981 gtccagacct gcattgcatt ccatctggat tctgcgcagc ctgcgctgcc cgttccggta
     5041 ttccttatga atcgactcgg cctcgtccgc gggaatcccg tgtttggtgc aataggtgtt
     5101 caagccattc gcatcacctt tgtccagata cttgcgcagg ccacggacgg atgtcaaggc
     5161 aaacccgaac cgtttttcca tttctgaaaa aactttttgc tgctcctcga tccggtttcc
     5221 ggcggatatc agtttctccg ccacttctct gaccacatgc cgtgacaacc tgacagtgcg
     5281 aagacgtgcc gaaaaacggt tgatcagctt ctggacctcg gtccgggatt cgtcgccgga
     5341 atcttcatca tcgcccggct cgctatccac cccgagcttt ttcagggcct tctcgatttt
     5401 cagaatctcc ttggaaagaa tttcaaaccg ctcttcatag gaggtctttt cttcctgcgt
     5461 cagattgatc acccggccgg tctcgatcag gtcatggagg gaagccttgc gtgccgttat
     5521 ccggtcgata atccttttga tctcgggaat cacggcatag gaatggacga ccacttcctc
     5581 aatctcctgt tcaccctgtt caatccgctt cgcaagaacg acttcctgtt cggctgaaag
     5641 gagcgaaacc tttccgatat ccctgagata atgcctgatg ggatcatcgg aagccggacc
     5701 atcatcatgg cggtagagtt tgcccgcaac ggcatggaca tcctgctcga tgacagccgg
     5761 gtgttcatcg gcatcctcca tctggtcatg ttgttcttcg tcttcgagat gggtgtctgc
     5821 atcatcttcg tggacttcat cgtgggtact ctgttcgaca ggagcagccc cgccgatctc
     5881 gcggacgtcc accacagtca cgccggcatc accgagagaa gtcagcagct cgtccatttt
     5941 ttcgggattg ccgagtgaat caggaagctc atcgttgatc atgtcatatg tcaggccacc
     6001 gcttttccgc cccagctcta tcaggcgctg caatacccgt gcctcgttat gatccagcat
     6061 tacccttgtc cgccttcagt tttgttcccg gtttcccgcc ggacggtccg catcctgcgg
     6121 gcaagatcat cctcgcgtcg tcggagcacc aggatctcct gctgcagggt ctggatggca
     6181 ttgtcatcac cgagcagttt ttgcctgcgc atctcgtcat ccagcaaagc cctttccctg
     6241 cggatcgttt tgatccagag tgtgtctatc gtgtccccaa actgcttctg ccaatcaatt
     6301 tcaagccggc cactggccat gtcttcctca aggatggagc ggatctctcc cggtgaaacc
     6361 cttgccagaa catcggccgc actccagact gtcttgtccc cggcgataga tgaaatgatt
     6421 tcgaggagtg atcgcgcacg ggaatcctcg attctcagtc cttttccgat ttccttgttg
     6481 atgtcgggcc aggcagacga atgcagtgca gccagccgca tgagatagag ttcaaaatca
     6541 ccatcctgcc cggccggact ctggccaaat ttagctaccg gtgcaggtgc cggccccatt
     6601 tttttcgccg tcgtcatcac aggcttttgg cgttggcgac gaaaatccag ctcaacctca
     6661 tcggaatcga caccggccag gagcgatgct ttttgcagaa agagcgacgt ttggacacca
     6721 tcctccattc cctcgagaaa ctggtagaca ccctgtaaga acatcatcgg cctgtcacca
     6781 ctgtcaactt cactcccttg atagatggta ttaatgacga aatcggccag cgggatggac
     6841 tggcgcagga gtgcctcgat ctgttcgcgg gtatgtttta gcgagatatc gaaggcatcc
     6901 agttcggaag gaagcagtac gacacgcgcc agacaaccag cctccatggc tggtctggct
     6961 gcggcaaagg cggcccgccg gcccgccttg tccccgtcaa aaagaaagat gatgtgcgag
     7021 gtcagcttct tgaggcgatt gaggtgccaa ctggtcagag cagtcccaag gggagccacg
     7081 acattggaaa aacccgcctg gtggagtccg atcacgtcga gatatccctc cacaatgatg
     7141 gccagatcct tgtcgccgat ggccgtccgg gcccggtcca acccgtagag gatctcgcgc
     7201 tttttgaata cgggactttc gggagagttg agatacttcg gctcacttcc gtcaaggacg
     7261 cgcccgccga aggcaacaac ccttccccgc tcgtccgcga tcgggaacat gacacgatcc
     7321 cggaaatagt catagatccg tcgtccctcc cgggatgcac gggaaagtcc gcactcgaca
     7381 agggtttccg cctcgtactg ctcgcggagc aacttcgagg agagcgcatc ccattggggc
     7441 aatgcgtatc cgagcctgaa atcatcgatg gtttcagcgc gcacccctct cccctcgaga
     7501 tacgccagcg ccgatttccc ggcaggcaga accttgaggg catggcgata cattgtggtg
     7561 gctttttcgt aaagatccat ccaccgcctg tgaatggcgg cctgggcatt ctcggccgga
     7621 ttgtccgagg ggagttcaat ccccgaagca tcggcaagaa acacgagggc ctcaccgaac
     7681 cccagatggt tgcgttcctg gacgtaatca aagacgtcac ccttttttcc gcacccgaaa
     7741 cagtagtacc tgtttttctc cgggtagacg gcaaacgaag gcgtccgttc attatggaac
     7801 gggcagagac ccttgtagat gctccctgcc cgtttcagcg caacgtcccg tccgatgaac
     7861 tcgacaaggg gcagggcttc gcgaatccgt tcgatcacct ctggcgaaac catccggcct
     7921 ccgtgcttct catgttgaca aacaaagctc cgccggccgc accaagagac ggcacaacgg
     7981 agctgttcgg cataaccaaa ccgtcctgcc catgcaggac ggggagatac ccgacatcgc
     8041 agatacagga gcgggtcttg ttgttcagtt acacgtgctt ggctttggcg cgagcaatct
     8101 tcatcttctt ctcgcgttta cgacgagcag cggcgtcctt cttcttgcgg atcatgctcg
     8161 gcttctcgaa atgctcgcgc tttttgattt ccgtcaacaa cccggccttc tcacactcct
     8221 ttttgaagcg tttcagtgcg agttcgatag gctcgctgtc cttgaccctg atacccagca
     8281 ttgtgattat cacctcttga tttgttgcgg caccctttgc agcaccggtt tatacatacg
     8341 aaagtctgtc caataatgca agaatcaccc cggaggccag gtcatgaacc tgccacccag
     8401 aagatgcgca tgcagatgga acaccgtctg accgccatcc tgattgcaat tcacaacaac
     8461 ccgatacccg ccctcggcta ctccatgctc ccgggccagt tttgccgctg tcgtcatgat
     8521 atatccgatc agcacaggat cctgcaccgc gtcaagcgaa gcgacatggt ccctggggat
     8581 gatcagaatg tgcatcgggg cctgcggttg aatatcgcgg aag
//
LOCUS       DAOSHE010000034         8393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7250_length_8393_cov_4.553250, whole genome shotgun sequence.
ACCESSION   DAOSHE010000034 DAOSHE010000000
VERSION     DAOSHE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_8393_cov_4.553250"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="mutL"
                     /locus_tag="PLD82_01865"
     CDS             complement(<1..975)
                     /gene="mutL"
                     /locus_tag="PLD82_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG64164.1"
                     /translation="MAGMQDRIMLLDPLVSARIAAGEVVEQPASVVRELVDNAIDAGA
                     TSISIELTDGGLTAIRVVDNGSGMGAADLARSVLKHATSKIRSLEDLDRVTSMGFRGE
                     ALFAIASVSRLRLVSNPGDGGHEIIVDNGKVGEVRAAAAPQGTIVEVTGLFRDIPARR
                     AFMKAPASEARTVRKILAEKMLAWPEIRFVLTSDGSDLVRSAGGDLVSRISDLYDSDL
                     ASSLLPLEHQEGEFGITGYLAPVHRNSASRRDQYCSINGRPVFLPAFSRAVATAYGNQ
                     LAPGRYAIVFMSITIPPSRINVNVHPAKREVRICDEGALFSAIVHAVRH"
     gene            complement(1182..3173)
                     /locus_tag="PLD82_01870"
     CDS             complement(1182..3173)
                     /locus_tag="PLD82_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOG64165.1"
                     /translation="MRNGIRYVVLLVLLCAAWGTLEAQENSALVVQQVIGEAQVKQGE
                     GTWGPVRVGMRLVPGMRVRAGSGSLLKASLGSDMSSLVTLRDGVMEVNELVLAASSRT
                     ARFTLQSGRMAAAVSGKYQNDFSVRTRTAVAGVRGTEYGVEYSDDEQSNVYVFRGQVS
                     VQSLDPLGQPRGESRSVNAGQKVVVRPTGEVTPVAPIAQTDENRFAMKVDKSPDKQDD
                     GNKSDDKKDDGKKDGGKSEDGNKSSGTSRDGSSSPSTGSGWTGSIGPVVIGDKTWTKM
                     LLAPQIQLGKLTLAFYVPVYYDANEPIWETSRWYNADEYDFENFGDFLHDLLLKFRFI
                     QYGAKGEEVFVKVGSIDDFVIGHGFIMNNYNNMLQFPSIRKIGLQLDFDFGAVGFESM
                     MADVHETKIFGGRAYVRPFTGFLDQLAFGLSYVTDIRTAPDINNYATVFGTGIDFEFP
                     LPSLGVLTWMVFADYATLGYKTTGTVGGKTDEDIGFNGGHGWTYGLKGRILFFKYQLA
                     FRNLSGGFIPEYFDSYYDVERQVRAETLIGANRSDFNGWLFETGFDLDTVGYVKLNFQ
                     EYYGMQGGVATTDNKLTFGAELKKGVVPKFHAMFLYERTHVIGSELFDSIFGDNTVTT
                     LSVFYEVSDGVEIVGTYRRFYEKGGGYTGSYSLETQMGL"
     gene            complement(3186..4529)
                     /locus_tag="PLD82_01875"
     CDS             complement(3186..4529)
                     /locus_tag="PLD82_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64166.1"
                     /translation="MMRKTLAVCAMLFLVFAVFASAEENPAATGGAPAATGEQPSGDS
                     ALPGAASGLLFGAFIGAATIDGVTYTQITLNPDLDLGGWGIGLDLNLEFDKDGKLRPG
                     EWDSWQAILNKIRYLRVGQKGETFFFRVGALKDVTIGHGTIMEGFANTLYYPDVRMPG
                     VQLDIDFGDFGFETFTENAMDLDIVATRVFIRPLGGLFKKLHIGFTFAADFDTLNPIK
                     TGKDKYEFEDNDASRVVRVYGIDAGLPLPSLGILEWMWFADYIQIVDKGNGFSTGLIG
                     EMAFIFNWKLEFNRFAPGFAGPFFDYFYMADRGTKYASLDQITDPYNGWKFKIWRNFS
                     IMKTDDLTVALQVSDTDGDAQKPMMIFDLHVDRKLLFDKVEFHLTYTKKDIDSFQKAF
                     TIEGLDTIINWQIGYMIAENVMISVNYTKTFMEDPENPGQIKGQEATSVQTQLKF"
     gene            complement(4666..5373)
                     /locus_tag="PLD82_01880"
     CDS             complement(4666..5373)
                     /locus_tag="PLD82_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64167.1"
                     /translation="MPGVVRIDPWLARLVSKNGFTRGALSIQAPHAVLTAVDFQNTDL
                     DTLPYWFAERRSVLMKNGVRTAGASMVVPVNHGRLVVGDRQHLVLFAGAEDSPDMLTL
                     HVTFIPSDVILKLASRTIRHPLEAEPVLDAIQKEINDPGYRGRVCVITPDTHTALTTA
                     REPDVWTTAFSSAVAADIATGQTKPSALASLAGQWIDLAYQDNRLCTSEIPFLCSMSE
                     AVARHDLYILLYARESV"
     gene            complement(5431..5823)
                     /locus_tag="PLD82_01885"
     CDS             complement(5431..5823)
                     /locus_tag="PLD82_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64168.1"
                     /translation="MKTGLGVILVALLAVAAVAQTTVDTAPAHAIQGTWKKTKAVDGL
                     LGGSVSIDMAAKTFSFAWKNRGQLTYSISDVKEDSETDMPRYTLTLKGNTGTMTVTVL
                     AFSSSTIYIGQTPHAGIAIWGAYTRSQP"
     gene            complement(5962..7302)
                     /locus_tag="PLD82_01890"
     CDS             complement(5962..7302)
                     /locus_tag="PLD82_01890"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOG64169.1"
                     /translation="MDDKIIVPGEYRNLKQLEPALLRVGGREYELPVVEGTEGERAID
                     ITNLRKASGHVTLDAGYQNTGSCTSNITYIDGEKGILRYRGIPIEQLAERASFVETAY
                     LLVHGSLPNRNEMRKFRNLLTQHSMIHEDMLTFFNGLPREAHPMAILSSMVNSLSIYY
                     PEVASTGEGGNGEELDIAVARLISKVRTIAAFSYKKSIGEPFVYPTNYTYCANFLHMM
                     FSSPIQKYKLNHIIIRALNLLLILHADHEQNCSTSTVRLVGSSKVNLYASICAGICAL
                     WGPLHGGANQKVIETLEYIRDNKIPVKKYIELVKDKDKPQRLMGFGHRVYRNYDPRAK
                     IIKKTCSEVLDFMKSDDPLLDIALELEEIALHDQYFIDRKLYPNVDFYSGIIYKAIGI
                     PTNMFTVMFAIGRLPGWIAQWKEMHDSAPFKIGRPRQIYNGPLIRDYVPIDERE"
     gene            complement(7435..>8393)
                     /locus_tag="PLD82_01895"
     CDS             complement(7435..>8393)
                     /locus_tag="PLD82_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG64170.1"
                     /translation="VSGGLVYFVLAGINFDDVARQLGDASVLLAGAASLVMLASHLLG
                     VMQWRVFLRAQRLEMSFSRCVVYYYTGQFFNNFLLSSVGGDIVRMFDVAYNEKVRKGR
                     VLASILVDRVFGLVCLIMIGLIALPFFIAREPSNLVPVMLLYAVFAGVMLLFWLFVMS
                     RYARHLTMHLVQRLPVARLRRAGFHFLRTFYCYRRTPGAFLRALGWGLTNQAVKMGVA
                     FITLAALTGSPGAVEPLWVIVFVPILGIVKVLPISIMGIGPHELAGRNLFGAVGVAAS
                     LTMSFLFLYQIVAILSNLACGVFLPGRRFGPAKDVDRSGLSR"
BASE COUNT         1866 a         2419 c         2424 g         1684 t
ORIGIN      
        1 atgccggaca gcatgtacga tcgccgaaaa gagcgcaccc tcgtcgcaga tccggacctc
       61 acgtttcgcc ggatggacat tcacattgat ccgggacggc gggatcgtga tcgacatgaa
      121 aacgatagcg tagcgccccg gagcgagctg gttcccgtac gccgtcgcaa cggcccggga
      181 aaaggccggc aggaagaccg gccgcccgtt gatgctgcaa tactggtcac gccgggatgc
      241 ggagttcctg tggacgggcg caagataccc tgtgatcccg aactcccctt cctggtgttc
      301 gagtggcagc agggatgacg cgaggtcact gtcgtagaga tcgctgatcc gtgaaaccag
      361 atcaccgccc gcggatcgga ccaggtccga accatcgctt gtcaggacaa accggatctc
      421 cggccaggcc agcatttttt cggccaggat cttccggaca gtccttgcct cgctggccgg
      481 tgccttcatg aaggcccggc gggccgggat gtcccggaag agtccggtca cctcgacaat
      541 cgtgccctgc ggggctgcag cggcccgcac ctcgccgacc ttgccgttgt ccacaatgat
      601 ctcgtggccg ccatctccgg gattcgacac gagtctcagc cgggaaaccg aggcgatggc
      661 aaagagagcc tctcccctga agcccatgct cgtcacccgg tccaggtctt cgagggatct
      721 gatcttggag gtcgcgtgct tgagaaccga gcgggcgagg tcggcagcgc ccattcccga
      781 gccattgtcc acaacccgga tcgccgtcag gccgccatcg gtcagttcga tgctgatact
      841 ggtggcaccc gcatcgatcg cgttgtccac cagttcccgg accaccgaag caggttgctc
      901 gacgacctca ccggcggcga tccgcgcact gaccagggga tcaagcagca tgattctgtc
      961 ctgcatcccg gccatcgaca ccctccgcgt ccttccgctc accgctgacg ggagcctgtt
     1021 caccaataaa agctacgatg gaatgagccc cgttgaaaag aaggatcctg acccgcccag
     1081 atggagcccg gtcctgccgg attgtaggcc cttgcagcgc cacaaaacac acaacccgcc
     1141 ccaagcggag cgggttgttg aaaagtgcgg cacaaagcgg atcagagacc catctgtgtc
     1201 tcgagactgt agctccccgt gtacccgcca cccttctcgt agaaacggcg ataggtaccg
     1261 acgatctcga caccatcgct gacttcgtaa aagacgctca gggtcgtgac ggtgttgtca
     1321 ccgaagatgc tgtcaaagag ctcactgccg atcacgtgtg tccgctcata gaggaacatg
     1381 gcgtggaact tgggaacaac accttttttg agctcggccc cgaaggtcag cttgttgtcg
     1441 gtcgtggcaa caccgccctg catcccgtag tattcctgga aattgagctt gacatacccg
     1501 accgtatcga ggtcgaaacc tgtctcgaag agccagccgt tgaagtcgct ccggttggcc
     1561 ccgatcaggg tttcggcacg gacctggcgc tcgacatcgt agtacgaatc gaagtactcc
     1621 gggatgaacc cgcccgagag gttgcggaac gccaactggt acttgaagaa gaggatccgg
     1681 cctttgagac cgtaggtcca accatggcca ccgttgaagc cgatgtcctc gtcggtcttc
     1741 ccgcccacgg ttcccgtcgt cttgtacccg agtgtcgcgt agtcggcaaa gaccatccag
     1801 gtcaggacgc cgagactggg aagcggaaac tcgaagtcaa tcccggttcc gaatacggtc
     1861 gcatagttgt tgatgtccgg agcagtccgg atatcagtga cgtaggagag accgaaggcc
     1921 aactggtcga ggaagccggt gaacggcctg acataggccc gtccgccaaa gatcttggtc
     1981 tcatggacat ccgccatcat gctctcgaag ccgaccgccc cgaaatcgaa atccagctgg
     2041 agaccgattt tccggatgga ggggaactgg agcatgttgt tgtaattgtt catgatgaaa
     2101 ccgtgaccga tcacgaaatc atcgatgctt ccgaccttga caaagacttc ctcgcccttg
     2161 gctccatact ggatgaagcg gaatttgaga aggaggtcat gcaggaaatc gccaaaattc
     2221 tcgaaatcat actcgtcggc gttataccag cggctggtct cccagatcgg ctcgttggca
     2281 tcgtaataca ccggtacata aaaagcgagg gtcaattttc cgagctggat ctgcggggca
     2341 agaagcattt tcgtccaggt cttgtcgcca atcacgaccg gaccgatgct ccccgtccaa
     2401 ccactcccgg ttgaaggaga ggatgaaccg tcacgtgagg tcccgctact cttgtttcca
     2461 tcctcgctct ttccgccatc cttctttccg tcgtcttttt tgtcatcaga cttgttcccg
     2521 tcatcctgct tgtccggtga tttgtcgacc ttcatcgcaa agcggttttc gtctgtctgg
     2581 gcaatcgggg cgacgggtgt gacttcgcct gtcggtctga cgacgacctt ttgtccggca
     2641 ttgacactcc gggactcacc acggggctgt ccgagggggt cgagactctg gacgctgacc
     2701 tgcccgcgga aaacgtagac attgctctgc tcgtcatcgc tgtactcgac tccatactcc
     2761 gtcccgcgca ctccggccac cgccgtccgt gtccggacag aaaagtcgtt ttgatatttc
     2821 ccggagacgg ccgccgccat ccgtccggat tgaagggtga atcgcgccgt cctcgaagaa
     2881 gccgccaaca ccagttcgtt gacttccatg acgccgtccc ggagcgtcac gagcgaggac
     2941 atgtcggacc cgagagacgc tttgagaagg gaaccagaac cggcacgaac ccgcattccg
     3001 ggaacaagcc gcatcccgac acggacaggc ccccacgttc cctcaccctg cttgacctgg
     3061 gcctcgccga tcacctgctg gacgacaaga gccgaatttt cctgagcttc aagcgtcccc
     3121 catgcagcac acagcagtac gagaagcacc acatatctga taccgttacg catctgtcag
     3181 cctcctcaga atttgagctg tgtctggacg gacgtggctt cctgtccctt gatctggccg
     3241 ggattctccg gatcctccat gaacgtcttt gtgtaattga ccgagatcat cacgttttcg
     3301 gcgatcatgt agccgatctg ccagttgatg atggtatcca gtccttcgat ggtaaacgcc
     3361 ttctggaaac tgtcgatatc ttttttggta taggtcagat ggaactcgac cttgtcaaag
     3421 aggagcttgc ggtcgacatg cagatcaaag atcatcatcg gcttttgagc atccccgtcc
     3481 gtatcgctca cctggagggc taccgtcagg tcgtcggtct tcatgatgct gaaattgcgc
     3541 cagatcttga acttccagcc attgtaggga tcggtgatct ggtcgagcga ggcgtacttg
     3601 gtcccgcggt ccgccatgta gaagtaatcg aagaagggac cggcaaaccc gggagcgaag
     3661 cggttgaatt cgagtttcca gttgaagatg aacgccatct cgccgatcag accggtcgaa
     3721 aaaccgttcc ccttgtcgac gatctggata taatcggcaa accacatcca ctcgagaatg
     3781 cccagggacg gcaggggcaa accggcgtcg atcccgtaga cgcggacaac gcggctggca
     3841 tcgttatcct cgaactcgta cttgtccttg cccgtcttga tcgggttgag ggtgtcgaaa
     3901 tcggcggcaa aggtaaaacc aatgtggagt tttttgaaaa gaccacccaa ggggcgaatg
     3961 aaaacgcggg tcgcgacgat atccaggtcc atggcgtttt cggtgaaggt ctcgaacccg
     4021 aaatcgccaa agtcgatatc aagctgtact ccgggcatgc ggacatccgg gtagtacagg
     4081 gtgttggcaa aaccctccat gatggtcccg tgtccgatgg taacatcctt gagcgcaccg
     4141 acacggaaga agaaggtctc gcccttctgt ccgacacgaa gatagcggat cttgttgaga
     4201 atggcctgcc aactgtccca ttcaccggga cggagcttgc catccttgtc gaactcgagg
     4261 ttgagatcga gtccgatccc ccaccctccg aggtcaagat cgggattgag cgtgatctgg
     4321 gtgtaggtca caccatcgat ggttgcggca ccgataaagg caccaaagag caatccggaa
     4381 gcggctcccg gcaaggctga gtcacctgaa ggctgttcgc ctgtggcggc cggagcgcca
     4441 ccggtcgcag ccggattttc ctcggcagag gcaaatacgg caaagaccag aaagagcatg
     4501 gcgcataccg ccaatgtctt gcgcatcata acgattcccc cgttacggtt ggataccagt
     4561 agctcgctgc cactgtacac cctgagtata gcacttcatc cacccatttc aaacaatgga
     4621 aggatcgcgg gacagacagg cataattcgg aattcagacc tgcgatcaga cggattcccg
     4681 cgcatagagc aggatataga gatcgtgacg ggccacagcc tcactcatgc tgcagagaaa
     4741 ggggatttcg gacgtgcaga gccggttatc ctggtacgcc agatcgatcc attgtccggc
     4801 cagggaggcc aacgccgatg gtttggtctg accggttgca atgtcggcgg ctaccgccga
     4861 agagaaggca gtcgtccaga catccggttc acgggcggtc gtcagagctg tatgcgtatc
     4921 cggggtgatc acgcaaaccc ggccgcggta tccgggatca ttaatctcct tctggatggc
     4981 atcgagaact ggctcggcct ccaaaggatg gcggatcgtc cgcgatgcca gcttgaggat
     5041 gacatccgac gggatgaagg tgacgtgcag ggtcagcatg tcgggcgaat cttcggcgcc
     5101 ggcaaagagg acaagatgtt gccggtctcc aacgacaagt cggccgtgat tgaccggaac
     5161 aaccatggaa gcaccggctg tccgcacccc gtttttcatg agcacggaac gtctctcggc
     5221 aaaccagtat ggcagggtat caagatcggt attctggaag tcgactgcag tcaggacggc
     5281 atgcggcgcc tggatggaga gcgccccgcg ggtaaaccca tttttgctga cgagacgggc
     5341 cagccagggg tcaatgcgaa ccacccccgg catgggcaca ggaacctcaa cagtctcgca
     5401 ataccggccc gtcacgtcgt gaaaccggta ttacggttgc gaacgggtat aggcacccca
     5461 gatggcaatc cctgcatggg gcgtctgccc gatatagatc gtcgaagagc tgaacgccag
     5521 aaccgtgacc gtcatcgttc cggtatttcc cttgagggtc agggtgtagc ggggcatgtc
     5581 ggtctcgctg tcttccttga catcgctgat ggaataggtg agctgcccgc ggtttttcca
     5641 ggcaaaggag aatgttttgg cggccatatc gatggacacc gatcctccga gaagcccgtc
     5701 cacggccttc gtttttttcc atgttccctg gatggcatgc gcaggagcgg tatcaaccgt
     5761 tgtctgcgcg accgcggcaa ccgccaacag tgctaccaga atgactccaa gacctgtctt
     5821 catgatgggt gtgcccccgt ttcgtattca atctaccatg tatatgtata ccaccgaatc
     5881 cgggggcgaa tcaagaccat tcttggacca aggttgttcc cccaaaaaaa gctctgccgg
     5941 aatgcgccgc attttggtga actactcccg ctcgtcaatc ggaacataat cccggatcaa
     6001 aggaccgttg tagatctggc gcggtcgtcc gattttgaag ggtgccgaat catgcatctc
     6061 cttccattgg gcgatccagc caggcaagcg cccgatggcg aacatcacgg taaacatatt
     6121 ggtcgggatt ccgatcgctt tgtagatgat gccagagtaa aaatccacat tgggatagag
     6181 ctttcggtcg ataaaatact ggtcatgcag ggcgatctcc tcgagttcga gggcgatatc
     6241 caaaagggga tcgtccgatt tcatgaaatc aaggacctcg ctgcaggtct ttttgatgat
     6301 cttggcccgc gggtcgtaat tgcggtagac gcggtggcca aagcccatca aacgctgagg
     6361 tttgtccttg tccttgacca gttcgatata ctttttgacc gggatcttgt tgtcccggat
     6421 gtattcgagg gtctcgatga ccttctggtt cgctccacca tggagggggc cccagagggc
     6481 acagatcccg gcgcagatgg aggcgtacag attgaccttg ctcgatccga ccagacggac
     6541 cgtcgaggtc gagcagttct gctcgtgatc ggcatggaga atcaggagga gattgagggc
     6601 ccggatgatg atgtggttga gcttgtattt ctggatcgga ctcgaaaaca tcatgtgcag
     6661 gaaattcgcg caataggtgt agttggtcgg atagacaaag ggctcgccga tcgatttttt
     6721 gtacgagaag gcggcaatgg tccggacctt ggagatcaac cgggcaaccg cgatgtccaa
     6781 ttcctctcca tttccgccct ccccggtact ggccacctcg gggtagtaga tcgagagcga
     6841 attgaccatg gacgagagga tcgccatcgg gtgggcctca cgggggagcc cattgaaaaa
     6901 ggtcagcatg tcctcgtgaa tcatcgaatg ctgcgtgagg aggttgcgga acttgcgcat
     6961 ctcgttacgg ttggggagcg agccgtggac gagaaggtac gccgtctcga caaaggatgc
     7021 ccgctcggcc aattgttcga tgggaatccc gcggtagcgg aggatcccct tttcgccgtc
     7081 gatataggtg atgttggatg tgcaggagcc ggtgttctgg taccccgcat ccagggtgac
     7141 atgcccgctg gccttgcgca gattggtgat gtcgatggcc cgttcacctt cggtcccctc
     7201 gacgacggga agttcatact ccctgccccc gacccgaagg agggccggtt cgagctgctt
     7261 gagattgcgg tattcgccgg gtacgattat cttgtcatcc atgagaactc cttaaggtat
     7321 cgctgtcgtg ttcggaccga cctgcggtat cgtggaatac aggcatcccg tctccgcccg
     7381 tctcggacag tgacacacga caggaatgca cacaaaccag ggtgagagaa tatattagcg
     7441 agacaagccg gatctgtcaa cgtcttttgc tgggccgaat cgccggcccg gaaggaagac
     7501 cccgcaggcc agattggaaa ggatggccac aatctggtac aggaagagga aggacatggt
     7561 cagactggcc gccaccccga ccgcaccaaa gaggttccgg ccggccagct cgtgcggacc
     7621 gatccccatg atgctgatcg ggagaacctt gacgattccg aggatcggga caaagacgat
     7681 cacccagagc ggttcaaccg ccccgggaga accggtcagg gccgccaggg tgatgaaggc
     7741 cacacccatc ttgaccgcct gattcgtcaa tccccacccc aaggcccgga gaaaggcccc
     7801 cggagtccgg cggtaacagt aaaacgtcct caagaagtgg aacccggccc tcctgagacg
     7861 ggcgaccggg aggcgttgta ccagatgcat ggtcaggtga cgggcgtacc gggacatgac
     7921 gaaaagccag aagagcagca tcactccggc aaaaacagca tagagaagca tgaccgggac
     7981 caggttggac ggctcccggg cgatgaagaa cggaagggcg atcaatccga tcatgatcag
     8041 gcagaccagg ccaaaaaccc tgtccaccag aatcgaggcc aggacccgcc ccttgcgcac
     8101 cttttcgtta taggccacat cgaacatccg cacgatatcg ccacccacgc tggagaggag
     8161 gaagttgttg aaaaactggc ccgtgtagta atagacaacg cagcgcgaga aggacatctc
     8221 cagtcgctgg gcccgcagaa agacgcgcca ctgcatgacc cccaaaagat ggctggcaag
     8281 catgaccaga ctggccgccc ctgccagcag gaccgaagcg tctccgagct ggcgagccac
     8341 atcgtcaaaa ttgattccgg cgagaacaaa atagaccagc ccgcccgaaa cag
//
LOCUS       DAOSHE010000035         8385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7262_length_8385_cov_3.792557, whole genome shotgun sequence.
ACCESSION   DAOSHE010000035 DAOSHE010000000
VERSION     DAOSHE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_8385_cov_3.792557"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PLD82_01900"
     CDS             <1..1891
                     /locus_tag="PLD82_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64171.1"
                     /translation="LLILLVLLSGTLSAAYNTSFIPTVVRQFSSPAEQKVVSELAKGN
                     LTSYSLVEAGFIASGATSNESLARYTSRYRDLLAKIRQDLPLMDENPRSRAKKLFDLM
                     FKHAMNAYQSEQTTLLEIFDAGHFNCVSSTLLYNLLCAEFGLKTRVAVVPGHVFSQVL
                     PGGTTWIDAETTSSAGFNPLRERTNPGGEDRVFIEDRGQSGGKSFVDTGRLVALLYYN
                     RGTLAHTQGRSSDAVALLMRALTIFPQHKESLENLLATMLEWAKKEGQTGNLDLAMRI
                     LTECDMALGSKNETQAMREGLVFNSAQAAAARGDFATAVRQIESWIASLPGQPTVQHK
                     TALRGYILQWAQQCMQQGKYSEMVSLIDRAARSGQDSFVDSMRLHLITEAAKNLSRTR
                     GYAQGYGFFNQHFPHSVENASIRQNRLHLYGLWITSEIESGKYEEAYVLNRSLLELYP
                     SNQDVRNNAAWVVQRWMASVSSNTSPAQSLPVMYGMYQKYREPVFLDELVNGTLRESN
                     RLLQIRQFEKAEVLVKKARDLGITGESGNKLAGMHRVILFNWSVFHAKRDEYMESIDI
                     GKRALKLFPGDGALETNLVSFYYNAGLTFINTRQYDRASRLITEGLAEFPGNPNLLKL
                     QQYLR"
     gene            complement(1981..3654)
                     /gene="lnt"
                     /locus_tag="PLD82_01905"
     CDS             complement(1981..3654)
                     /gene="lnt"
                     /locus_tag="PLD82_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG64172.1"
                     /translation="MVRQQVMNFFHTRVYEIGHFRWKKWSLGLLLAVLSGVLLGLAFP
                     LTSFFPLAWVAFVPLLVMQRGVPVWRMWFYAFMAGLSFWLVHASWLKVFHPLSLVTAT
                     PALALYFSLPFVFMAVFRRRMNNRFPLLQAFLFATLWIGVEYFRSTGFLGFSWGVVGY
                     SQQYFLPVLQIADIGGVWSVSCIVLFANAVIANWLVEGYPIVRKRFLVLAGVLVFVLG
                     YGVFRLTEEPSGPVKRVALVQAFVDPAVDWKQARADATMQKIEEMTIAAGQHEPRPDL
                     VVWSETLSSPSALWYYTHLRKAAAGSSQRAIGEWFMGMPARAGRPVLLTAPHKEFVAY
                     TNEKGLLRHEIESYNAAFLVTETTNIVDRYYKINLVPFGEWFPYKKEFPWIAKILEKT
                     IASDFTPGKRYTAFSMPQGRFGVLICFEDIFGEICRQLVLAGAEYLINTTNDYWSKSI
                     QSQEQHAAMAVLRAVENRRFLVRAANTGVTCVVDPWGRMLQRLPNEETGILHSGISLM
                     RGRGPTFYTLHGDWAGIAGAWACALAGVAGMGVWLTASGRRIKKLILKK"
     gene            3745..4713
                     /locus_tag="PLD82_01910"
     CDS             3745..4713
                     /locus_tag="PLD82_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64173.1"
                     /translation="MRTKTHRFLIALIAIILTSIIPPGHHQANAALVFEEEIGPVVLM
                     MEFELFYLPLGLVLPLASDSIPRLETDSELEIYGYLLTRFFLPRTIVIEGSIYPMPLL
                     GAGIRKYAPEFYADCEIFEGFNIIQSITSSFFEEPWAASVFFGNVVAYAPAGRPASAT
                     NSDTIQGKGYSGLVTSYGNWHIRDNEMIRDDWLEIELKLKGEIIDGKKTISWSYKIGT
                     RLHSNDDIKSWFYLGLRRDHTDFDFHDFTLFENSFAELLLRLENETARPQGIRFVFGK
                     KFPLRDSPLLPELNIGFQYNFRSPYTGELVPEDQRFHLVISPNLRF"
     gene            complement(4710..5642)
                     /gene="xerD"
                     /locus_tag="PLD82_01915"
     CDS             complement(4710..5642)
                     /gene="xerD"
                     /locus_tag="PLD82_01915"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOG64174.1"
                     /translation="MTTKNNGGDRGLLEDFLAWAGVEKGLSPKTLEAYASDLHLFSAW
                     CDTAGRAWLDADEDLVVAWLWSLKQAGSAPTSVARRLVTLRQLYKFLLLEERIETNPV
                     AFLDSPRTGRPLPVCLTRAEVERLTTWYPPDTVRGLRDRTILEILYSCGLRISELLNL
                     RMGNIDLANGFLTVEGKGSKERIVPVGQRALGLLRDYLGMARSGLSGKTVTDIVFLNA
                     RGGQLSRMGAWKIVHAAVIGAGIDKPVSPHTLRHSFASHLLENGADLRAVQELLGHAD
                     IATTQIYTHLAREYLVRIHAECHPLEIGGSAGPS"
     gene            complement(5626..6843)
                     /locus_tag="PLD82_01920"
     CDS             complement(5626..6843)
                     /locus_tag="PLD82_01920"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOG64175.1"
                     /translation="MLDRLEDAIEAIRNGRMVVVVDDESRENEGDLVMAAERTTPEAV
                     NFMITEGRGLICVPMDAGIAVGLDLRPMVSDFENSDPKGTAYTVSVDADALVSTGISA
                     ADRSRTIQRLADLGSKGSDFRRPGHIFPLAARKGGVLVRAGHTEASVDLVRMAGLNPV
                     AAICEILNPDGSMMRLTGLREFADRHGLLLISVEQIIRHRRRNENLVRRVADAKLPTD
                     FGEFSIAIYGNEIDALEHAALVYGDITGAEDVIVRVHSECLTGDVFSSLRCDCGQQLH
                     EAMRMIAEAGRGVVLYMRQEGRGIGLGNKIKAYHLQDQGYDTVEANEALGFADDLRDY
                     GIGAQILRDLGLTSIKLVTNNPRKLVALEGYGLSITGRVPLVIKPGEKNRFYLQTKRD
                     RMQHMLPKDDDEK"
     gene            complement(6843..7511)
                     /locus_tag="PLD82_01925"
     CDS             complement(6843..7511)
                     /locus_tag="PLD82_01925"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOG64176.1"
                     /translation="MFSGIISAVGSILASRQTGAGREISVQADPSWLDGVQRGESIAI
                     NGACQTVTEFRTGVFTVFASNETLSCTNLGTLGQGGKVNLERSLVVGGRIDGHLVSGH
                     VDTRARIVFLRRYDNTIELGVELPLSVAPLVAPKGSLAVDGISLTVHTVRGNTAGFVL
                     IPESVGRTTVPDWEPGYEVNLEVDLVARYLARIMECRGMTGDARFAAVLEESGFGPGI
                     RRED"
     gene            complement(7511..7831)
                     /locus_tag="PLD82_01930"
     CDS             complement(7511..7831)
                     /locus_tag="PLD82_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64177.1"
                     /translation="MRIAAYIMLGLSIGGLLTIIIDGFAPGTNFFHLLALGISLLMMG
                     LAVALWWGARKGPWIRSLLIVLMVLVVAVWGFVGYRCYRISVMPAENDQAAGDALNNS
                     GLYQ"
     gene            7934..>8385
                     /locus_tag="PLD82_01935"
     CDS             7934..>8385
                     /locus_tag="PLD82_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG64178.1"
                     /translation="MTIQRFTLLYHQYQPELSRFASWLTGESWIADDILQDALVSAIK
                     HLDALPSGISFRAWMFMNIRFAWSRHYRCERREYRNFCLYHRMTDPLKEDNTPDTDQI
                     LRPWKQHVAKALGTLHPMYRRPLILHEILGMNCASVSEAEQVYEGTIKS"
BASE COUNT         1999 a         2525 c         2160 g         1701 t
ORIGIN      
        1 cttgctgatc ctgctcgtcc tcctgtccgg caccctttca gcggcataca atacttcatt
       61 catcccgacg gtcgtccggc aattctcgtc acctgccgaa caaaaggtcg tgtcggagct
      121 tgccaagggg aacctgacca gttactcgtt ggtggaagcg ggtttcattg cctccggtgc
      181 taccagcaat gaaagccttg cacgctacac gagccggtac cgggatctct tggcaaaaat
      241 ccgccaggac ctgcctctga tggacgaaaa tccccgttca cgggctaaaa aactctttga
      301 cctgatgttc aaacatgcca tgaatgccta ccagtccgag caaaccacgc tcctggagat
      361 ctttgacgcc ggacatttca attgcgtctc ctcgaccctg ctctacaatc tcctctgcgc
      421 tgagttcggc ttgaaaacca gagtggccgt tgtcccggga catgtcttca gccaggtcct
      481 tccgggagga actacatgga tcgatgccga aacgacgtct tcggcggggt tcaatcctct
      541 ccgggaacga accaatcccg gtggcgaaga ccgtgttttc attgaagacc gtggacaatc
      601 tggtggaaag agcttcgtcg acaccggccg tcttgtcgcc ctcctctact acaacagggg
      661 aacactggcg catacccagg gacgctcgtc cgatgccgtc gccctcctga tgcgcgcttt
      721 gaccatcttt ccgcaacaca aggagagtct tgaaaacctt ttggcgacca tgctggagtg
      781 ggccaaaaaa gaaggacaaa ccggaaatct cgatctggcg atgcgcatcc tgacggaatg
      841 cgacatggcc cttggctcca aaaacgaaac gcaggccatg cgcgagggtc tcgtcttcaa
      901 ctcggcccag gctgctgcag ccagggggga ctttgccacc gccgtgcgtc agatcgaatc
      961 ctggatcgcg tccctcccag ggcagcccac agttcagcac aaaacagcct tacggggcta
     1021 catccttcaa tgggcccaac agtgcatgca gcaggggaaa tacagcgaga tggtgtcgct
     1081 gattgaccgg gctgcacgct cggggcagga cagctttgtg gattcgatgc gactccactt
     1141 gattaccgaa gcggccaaaa atctgtcgcg cacccgaggc tacgcccagg gatacggctt
     1201 tttcaaccag cactttcctc actccgtcga aaatgcctcc atccgacaaa acagactgca
     1261 tctgtacgga ctctggatca cctccgaaat cgagtccggc aaatacgagg aagcttacgt
     1321 cctcaaccgt tccctcctcg aactctaccc gtccaaccag gatgtccgta acaatgcggc
     1381 ctgggtagtc caacgctgga tggcctcggt gtcgtccaat acatcacccg cacaatcgct
     1441 tccagtcatg tatggcatgt accagaagta ccgtgagccg gtctttctgg acgaactggt
     1501 caacgggacc ctgcgcgagt caaaccggct cctgcagatc cgacagtttg aaaaagccga
     1561 agtccttgtc aaaaaggccc gtgatttggg aatcaccgga gaatccggaa acaagctggc
     1621 cggcatgcac cgggtgatcc ttttcaactg gagtgtcttc cacgccaaac gggatgaata
     1681 tatggaatcc atcgatatcg ggaaaagggc cctcaagctc ttcccggggg acggcgccct
     1741 tgaaacgaac ctggtcagtt tttactataa cgccgggttg acctttatca ataccaggca
     1801 gtacgatcgc gcttcaagac tgattacaga aggactggcg gagtttcccg gaaaccccaa
     1861 tctcctcaaa ttgcaacaat acctcagata acccggtttg cgcaaggaag ccggaaggaa
     1921 ccctgtactg gaatggccgt ctcctggcat gcccatcccg gccatgtcag gagacggagt
     1981 tcactttttc aggatgagct ttttgatgcg acgtccagac gcagtcaacc agacacccat
     2041 tccggcaact cccgccaacg cacaggccca agcgcccgct atccccgccc agtccccatg
     2101 cagggtatag aatgtcggac cacgaccacg catcagactg atcccggaat gaaggattcc
     2161 ggtttcctca ttgggcaggc gttgcaacat ccgtccccag ggatcaacaa cacaggtcac
     2221 gccggtattg gccgcacgaa ccagaaagcg cctgttttca acggcacgca gaacagccat
     2281 ggcggcgtgc tgctcctgtg actgaatgct cttggaccaa taatcattgg tcgtattgat
     2341 cagatactcc gccccggcca ggaccagttg gcgacaaatc tcgccaaaga tatcctcaaa
     2401 gcaaatcagc acgccaaaac gcccctgtgg catggaaaac gccgtatacc gctttccggg
     2461 tgtaaaatcg ctggcgatcg tcttctcgag gatctttgca atccagggaa attctttctt
     2521 gtagggaaac cattctccaa aaggaaccag attgatcttg taataccgat cgacaatgtt
     2581 ggttgtttcg gtcactaaaa aagcagcatt gtaggactcg atctcgtgcc gcagcaggcc
     2641 cttctcgttt gtgtaggcca caaactcctt gtggggtgcg gtcaggagaa cgggcctccc
     2701 ggcccgggca ggcatcccca tgaaccactc accgatcgcc cgctgactgc tcccggcagc
     2761 cgcttttcgc agatgggtat aataccagag agccgatgga gaactcaagg tctcagacca
     2821 gaccaccaga tctggccggg gttcatgctg ccccgctgcg atggtcatct cttcaatctt
     2881 ctgcatggtc gcgtctgcac gcgcctgctt ccagtcaaca gccggatcga caaaggcttg
     2941 aaccagcgcc acacgtttga ccggcccgct tggctcttcc gtcagacgga acacgccgta
     3001 gcccaaaacg aacaccagga ctccggccag caccagaaac cgtttgcgga caatcgggta
     3061 tccctcaacc agccaattgg caatcacggc attggcaaag agtacgatgc atgaaacact
     3121 ccagacccct ccaatatcgg cgatctggag aactggaaga aagtattgct gggaataccc
     3181 gaccactccc caggagaatc caaggaaccc ggtcgatcgg aagtattcaa cgccaatcca
     3241 gagagtcgca aacaaaaaag cctggagcaa ggggaaccgg ttgttcatcc ttcgacgaaa
     3301 aaccgccatg aagacaaaag gcagggagaa atacagggcc agggccgggg tggccgtgac
     3361 gagcgaaaga gggtgaaaga ccttgagcca ggaggcgtgg accagccaga aggaaagccc
     3421 ggccatgaac gcatagaacc acatccgcca gacggggaca ccccgctgca tgacgaggag
     3481 aggcacgaag gccacccaag ccagcgggaa aaaacttgtc agcggaaagg ccagacccag
     3541 gaggactccg gacagtacgg caagcaacag cccgagactc cacttcttcc agcgaaagtg
     3601 tccgatttca tagacccgtg tgtgaaaaaa attcatgacc tgctgccgaa ccatgtttgc
     3661 atacctcgcc agaaaatgtt agtcaacgga tctctggctg tcaatccgct tgaccatgtc
     3721 cgggctcgat actagcatac tgtcatgaga acaaagactc accgctttct gatcgcgctc
     3781 atcgccatta tcctgacaag catcatccct cctggacacc accaggccaa tgccgccttg
     3841 gtcttcgagg aagagatcgg tccggtggtc ctgatgatgg agttcgagct tttttacttg
     3901 ccgctgggtc tggtgctgcc actggcgagc gattcgattc ccaggctgga aacagacagc
     3961 gaactggaaa tctacggcta tctcctgaca cggttctttc tccctcgaac gatcgtgatc
     4021 gaagggagca tctacccgat gcccctcctc ggtgctggca tccggaagta tgcccccgaa
     4081 ttttatgctg attgcgagat ttttgagggc ttcaacatca tccagtccat cacatcctca
     4141 ttttttgaag aaccttgggc cgcgtcggta tttttcggaa acgtcgtcgc gtacgctcct
     4201 gccggccgtc ccgcctcggc aaccaactct gacacaatcc agggcaaggg ctacagcggt
     4261 ctcgtcacca gctacggaaa ctggcacatc cgcgacaacg agatgatccg ggacgactgg
     4321 ctggagatcg aactcaagct caagggcgag attatcgacg gaaagaagac cattagctgg
     4381 agctataaaa tcggaactcg gctgcattcc aacgacgata tcaagtcctg gttttacctc
     4441 gggctgcgcc gggatcatac cgattttgat ttccacgact tcacactctt cgaaaacagt
     4501 tttgccgaac tcctcttgcg gttagagaat gaaacagcac ggccgcaggg tatccgcttt
     4561 gtcttcggga aaaaattccc gctccgcgat tctccgctcc tccccgagct caatatcggt
     4621 ttccagtata atttccgttc accgtatacc ggagagcttg tccccgagga ccagcgcttc
     4681 catctcgtga tcagcccaaa cttgagattc taggatggcc cggcagaccc gccgatctcg
     4741 agcgggtggc actcggcatg gatccggaca agatactccc gggccaggtg cgtatagatc
     4801 tgggtcgtgg caatgtcggc atggccaagg agttcctgga cggcgcgcag atccgcgcca
     4861 ttctcgagaa gatgactggc gaaggaatgt ctgagcgtat gtggtgaaac cggtttgtcg
     4921 atcccggcac cgatcacagc ggcatggacg atcttccagg cacccatccg cgagagttga
     4981 ccgccacggg cattgagaaa gacgatgtcg gtaacggttt tccccgacaa acccgatctt
     5041 gccatcccga gatagtcgcg caacaaaccg agggcccgtt gcccgaccgg cacgatccgc
     5101 tccttggagc ccttgccctc gacagtcagg aatccgttgg caagatcgat attccccatc
     5161 cgcaggttca agagctcgga gatgcgcaga ccgcaggaat agagaatctc gaggatggtc
     5221 cggtcacgca gtcctcgtac cgtgtcaggt ggataccagg tcgtcaaccg ttcgacctcg
     5281 gcccgtgtca ggcaaaccgg aagcggtcgt ccggtgcgtg gcgagtccaa aaacgcgaca
     5341 ggattggtct cgatacgttc ctcaagcaaa agaaatttgt agagctgacg aagcgtgacc
     5401 agtctccggg caacactcgt cggagcagat ccagcctgct tgagcgacca gagccaggcc
     5461 acgaccagat cttcgtccgc atccagccag gctcgaccgg ccgtatcgca ccaggcggaa
     5521 aacaggtgaa gatcactggc ataggcttcg agggtctttg gggagagtcc tttttcgaca
     5581 cccgcccacg ccaagaaatc ctcgaggagc ccccggtcgc caccattatt tttcgtcgtc
     5641 atcttttgga agcatatgct gcatgcggtc ccgctttgtc tggagataga aacggttctt
     5701 ttcaccgggt ttgatgacca ggggaaccct gcccgtaatg gaaagcccgt agccttcgag
     5761 ggcgaccagt ttcctcggat tgttggtgac aagtttgatc gaagtcaagc cgagatccct
     5821 gaggatctgt gccccgattc cgtaatcacg gagatcatcg gcaaacccga gagcttcgtt
     5881 ggcctcgacc gtatcgtatc cctgatcctg cagatgatag gccttgatct tgttgcccag
     5941 accgattccc cgaccctcct gtcgcatata gaggaccaca ccacggcccg cttccgcaat
     6001 catgcgcatt gcctcgtgga gttgctggcc gcaatcgcag cggagggacg aaaacacatc
     6061 cccagtcaga cactccgagt ggactctgac gatcacgtct tcggcacccg tgatgtctcc
     6121 gtacaccaaa gccgcgtgtt ccagtgcatc aatctcgttt ccgtagatcg cgatactgaa
     6181 ctccccaaaa tccgtcggga gttttgcatc agcaacgcgc cggacaaggt tttcattcct
     6241 gcgtcgatgc cggataatct gctcgaccga gatcaagaga agaccgtgcc ggtcagcaaa
     6301 ctcgcgcaag ccggtcaggc gcatcatgct cccgtcggga ttgagtatct cgcaaatcgc
     6361 tgcaacagga ttgagcccgg ccatcctgac gagatcgacc gaagcctccg tatggcctgc
     6421 cctgaccagc accccgccct tgcgggccgc cagagggaaa atatgcccgg gtcggcgaaa
     6481 atcactcccc ttcgagccaa gatcggccag tctctggatt gtccgggaac ggtcagccgc
     6541 ggagatgccc gtgctgacaa gagcatcggc gtcgaccgaa accgtatacg ccgtaccctt
     6601 cggatcggag ttttcaaaat ccgaaaccat cggcctgaga tcaagcccga cggcaatccc
     6661 cgcatccatg ggcacacaga tcagcccacg accttcggtg atcatgaagt tgacagcttc
     6721 cggggttgtc ctttctgcgg ccatgacgag atcgccttca ttttccctgg attcatcatc
     6781 gacaacaaca accatccggc cattccgtat tgcttcaatg gcatcttcaa gtctgtcaag
     6841 catcagtctt ctctccgtat tcccgggcca aagccacttt cctcaagtac ggctgcaaaa
     6901 cgggcatcac ccgtcatgcc acgacattcc atgatccgtg ccaggtaccg ggcgacaaga
     6961 tcaacctcga ggttgacttc atagccgggt tcccagtccg gaaccgttgt tctcccgaca
     7021 ctctccggga tgagcacaaa ccctgccgta ttcccgcgga cggtatgaac cgtcagcgag
     7081 atgccatcca cggccaacga acccttgggg gcaaccagcg gggccaccga caggggaagc
     7141 tcgacaccca attcaatcgt attgtcatac cgacggagaa aaacgatcct ggcacgcgta
     7201 tcgacatggc cactcaccag atgcccgtcg attctgccgc ccacgacgag ggaacgttcg
     7261 agattgacct tgccgccctg accaagagtt cccaggttcg tacaagaaag ggtttcattt
     7321 gaagcgaaga ccgtaaagac tccggtccgg aattccgtca cggtctggca ggccccgttg
     7381 atcgcaatac tctctccacg ctgaaccccg tccagccagg aaggatcggc ctggacgctg
     7441 atctcgcgtc cggcgcctgt ctgtctcgac gcgagaatgc tccccacggc actgatgatt
     7501 ccactgaaca ctattggtac aatcctgaat tgttgagtgc atctccggcg gcctgatcgt
     7561 tttcggcagg catgacggag atccggtaac accggtatcc gacaaatccc cagactgcga
     7621 caacgaggac catcaggaca atcaggagag aacgaatcca gggccctttt cgggcccccc
     7681 accacagagc cacggcaaga cccatcatca gaagagaaat tcccaaggcc aacaggtgga
     7741 aaaaattggt ccccggtgca aaaccgtcga tgatgatggt cagcagcccc ccgatcgaga
     7801 gaccaagcat gatataggca gctattcgca taggcggctc ctcaaaccgt aaaattacgc
     7861 aaatttttat caaatttttg gcactttttt ggcttcgtcg gcctgtatat atatgaaccc
     7921 gcaaggagtc gtgatgacca tccaacgctt cacgcttttg taccatcagt atcaacccga
     7981 actttcaagg tttgcctcat ggctaaccgg agaatcctgg atagcagatg acattctcca
     8041 ggatgccctc gtcagtgcca tcaagcacct cgatgcactc ccgtccggaa tcagcttccg
     8101 cgcctggatg ttcatgaata tccgctttgc ctggtcaagg cattatcgct gtgaacgcag
     8161 ggaatacaga aatttttgtc tctatcacag gatgaccgac cccttgaagg aggacaatac
     8221 cccggatacc gaccagatcc ttcgcccatg gaagcagcat gttgcaaagg cacttgggac
     8281 acttcacccg atgtacagac gcccgctgat cctgcacgag atcctcggga tgaattgcgc
     8341 gtcagtctcc gaagcagaac aggtctatga gggaaccatc aagtc
//
LOCUS       DAOSHE010000036         8282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7377_length_8282_cov_4.468336, whole genome shotgun sequence.
ACCESSION   DAOSHE010000036 DAOSHE010000000
VERSION     DAOSHE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7377_length_8282_cov_4.468336"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLD82_01940"
     CDS             complement(<1..240)
                     /locus_tag="PLD82_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64179.1"
                     /translation="MNHNRFRISTIIFWGAVWGLAEATLGYVLHLLPGLVSGAVMFPV
                     GLFCMTRAASQSRASDVFGVALIAAAIKSINLLFPQ"
     gene            complement(237..794)
                     /locus_tag="PLD82_01945"
     CDS             complement(237..794)
                     /locus_tag="PLD82_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOG64180.1"
                     /translation="MITIVTGPVNSGKSTTLVSLLFEEQSSGGFVQRKLTRETDGFVT
                     GYDLVSLRDSSVIPFARALDDLPSDWDGGPQLGRFSFSVSGTASAIACIRRDLADGCH
                     PVAIDEIGRLELMGDGLAPVLDQLKTTKAILVVRDCFLTAIMERLNILPAGCRIITCV
                     SSERRDTIPYSHNSTQTFCSERSLV"
     gene            complement(791..>1604)
                     /locus_tag="PLD82_01950"
     CDS             complement(791..>1604)
                     /locus_tag="PLD82_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOG64181.1"
                     /translation="GTTLVMAFPEPVIKQPAEHQGDAPVDSLRKALVAATPSFPIQQI
                     FAGPIRSFALSQHGGVASTVWPIGSCGHGDSAFFSRPGLTTDEAAQKLFSDQPFDASL
                     AMAAINSALPTDGLITENINARDLIIREASGGVLGLIGHFCFLEKFRPHFADLLVFEL
                     EPRPGDIPASEIPKRLREADVVAVTGTTLINGTIETILDSVRPDARIVMLGPSTPLAP
                     VLLEHGIHAICGTVVDDPEVVARNAMLAGSPHDHCGTRQIVLTDSASREQTA"
     assembly_gap    1605..1704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1955..5062)
                     /locus_tag="PLD82_01955"
     CDS             complement(1955..5062)
                     /locus_tag="PLD82_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG64182.1"
                     /translation="MTPTLSAKELAKKKLAVLVREFEELENTAGLKGQSEAQARIWIN
                     KALEAFGWNIANPAEVILEYSIKGRAARRIKTEGFHHNRPDYALLVNGKRILYIDAKR
                     FDIYIQEHSTSAFQARTYGWSAGMSLSYVMDFQELAIYDCRFKPAPQDNAKVARLAYF
                     HYKEYIPRFDELWEYFGHEAIINGSLEHINPQDKLPRGAMTLDTDFETNLSLWRKELA
                     KSVLRYGKIRDAKLLSAVAQRILDRIIFIRFCEEIGLEEYGTLKAIVHDQDGFWPAFM
                     KETETRWRGVYDGVLFPHSEEDDPTGVETVINDIWIKGNVFKTICAGLYMPSPYCFEV
                     IPIDLLGGIYERYLGKRLKVVGSDVEDEFKPEHQRTRGAVYTPDWVIRRILSRVLDPL
                     TTGKTPADILALKIIDPSCGSGSFLLGAFAFLEDKIVDWCRANRDAAGEWAIIDGDEV
                     RLNRATARAIISGCLHGVDIDPEAIEIARMSLALRCVERTALEEAETPKDLLHGIGLN
                     IRHGNALVGIDGNIALDPQAISQVIPFDWTSTTHGFGAILANGGFDAVIGNPPYIEVK
                     HYKKWMPLMYDYLKSGIYETAREGKTDIAMPFIEKAVSILKPSGRMGYIVQNRFFRTD
                     YGAATRNFLAKNRLLEGVDDFRDIQVFSGRTTYTAILVLAKNTAQCRYRTYASIDDAI
                     RDKPSLSCKISLTSLDADIWCLDKPDLSALHKKLAVQFGTIGTHAELSITVGLQSLWN
                     KVYQLQPVSATETTVTATNGLGEEVTLELAAVRPLCRNRGFYPFRRNNTHAWIIFPYT
                     INNGQADEIGWTRFQQAFPMAANYLQEHKKALSQNVELNPEKNRWHLYTRPQNLVSQT
                     RPKVLFPMTIQDTMASLDETGTTYQDNVNVNSLSISTDSLIHLKAVCAIFNSSTFNAL
                     AILKTGLNDNGWHKFNRQYADRVPLPWHKLCDPVHAGPLAQLADAISAAQVTLFAVEG
                     EGQKAAATSALARLWADLDSAVETLYELSDEDRAIIRAAPRQVDRIQLLYRQSDGIAD
                     ETDEG"
     gene            complement(5311..>5733)
                     /locus_tag="PLD82_01960"
     CDS             complement(5311..>5733)
                     /locus_tag="PLD82_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG64183.1"
                     /translation="EALEKKLEAMDPRPDIAPVLLAQGSPLPAGPFDLVHLSMVLHHV
                     EEAGQLIADLARRLAPGGQLAIFDLAPDGGLFHDDNAGVFHNGFDQDQLRQWFAEAGL
                     ANIRIIEAHRMARNRTAADGSACTLEFPILLATGMRVG"
     assembly_gap    5734..5833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5834..6227)
                     /locus_tag="PLD82_01965"
     CDS             complement(<5834..6227)
                     /locus_tag="PLD82_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64184.1"
                     /translation="MHALRIVSLVSLVALFSLVACSENDAVPPVSAADAIKLADSVRT
                     VIKSEVDMAKNTLVTGKAWTNSESKTIVSGSAVDSSQQITIDATVSLTNLALADGIWT
                     VSGSCSYKFEQNVNNDETVVTFNVLAAPF"
     gene            6464..7189
                     /locus_tag="PLD82_01970"
     CDS             6464..7189
                     /locus_tag="PLD82_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64185.1"
                     /translation="MIRPLFTISILCLVVVFAGQAQASMTVLDVYQSLPKEAFALDGT
                     QHRYEIAQLGNKWKTKSIVGYEMDVVVDLENGYIEIVDRGTGGGFINHELSIFDSKKR
                     GRVVALNIFGGDGTRASSAGIKFFVREASSWKDVTAEVLPGIDWTMFYQSNQWNMEDL
                     KKGVQIAAREKIELVRYKIPRIGTTLRASFLRENIIIRGQYTTSGLSPEESRILASMV
                     NTLARSRIMLRWDKENGVFRLPD"
     gene            7288..>8282
                     /locus_tag="PLD82_01975"
     CDS             7288..>8282
                     /locus_tag="PLD82_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG64186.1"
                     /translation="MTSIIKNGILVTETHAQHGDILLVDGKIAAVGDLRNESAETVYD
                     ARGCYVMPGGIDPHVHLSLPVGGGLLSADDFENGTRAALAGGTTSVIDFVTPEHGQDL
                     VTALRARRAEADHHVLCDYGLHMSVIEWRDSIPEEMARSAELGAPSFKTYLAYKKAIG
                     LDDAEFVRVLDAAARGRYLVTAHCEHGDIIDYLQKNFVEEGKTAVRWHPRSRPAAVEE
                     EAVTRAIALAGTLGASLYIVHLSTAGGLEAAAAARAAGQALYVETCPHYLLLNEDAYE
                     GSFDETARYTMSPPLRGPEHSRALWEGIAAGLVDVLATDHCPFCTSGQKDRGRED"
BASE COUNT         1766 a         2068 c         2448 g         1800 t
ORIGIN      
        1 ctgcgggaac agaagattga tcgacttgat ggcggctgca atcagtgcca caccgaagac
       61 gtcgcttgcc cggctctggc tggcagcacg tgtcatgcaa aagagtccaa ctggaaacat
      121 gacggctcct gagacaagac ccggcagaag gtgcagtacg tatccgagtg tggcctcagc
      181 caagccccag acagcgcccc agaaaatgat ggtagaaatg cgaaaacggt tgtgattcat
      241 acaaggctcc tttctgaaca aaaagtttgt gtgctattgt gagagtatgg gatcgtgtcc
      301 ctcctttcgg atgaaacgca tgtgatgatc cggcagccag cgggcagaat attcagacgc
      361 tccatgatgg cagtcaggaa gcagtcacga acaacaagga tggctttcgt ggttttcagt
      421 tgatcaagca caggagccag gccatcaccc atcagttcaa ggcggccaat ctcgtcaatg
      481 gcgacaggat gacacccgtc ggcaagatcg cgtcggatgc aggcaatcgc tgaagccgtt
      541 ccggagacgg aaaacgaaaa ccgtcccagt tgtggacccc catcccagtc actcgggagg
      601 tcatcgaggg cgcgcgcaaa agggatcacg gagctgtccc gcaggctcac caggtcgtat
      661 cccgttacga atccgtcagt ctcccgggtg agcttacgct gcacgaaacc gcctgaggac
      721 tgttcctcaa aaagaagcga aacgagggtc gtcgactttc cggaattgac agggcctgtc
      781 acgatggtga tcatgccgtc tgctcccggc tggccgaatc ggtcaggacg atctggcgcg
      841 ttccgcagtg atcgtgcggc gacccggcca gcatggcgtt gcgtgcgacg acctcggggt
      901 catcgacaac ggttccgcag atggcatgga ttccatgctc aagaaggacc ggtgcaagcg
      961 gggtggaggg cccaagcatg acgatgcgtg catcgggccg gaccgaatcc aggatggttt
     1021 cgatggtccc gttgatcagg gtcgtgcccg tgacggccac cacatcggct tcgcggagtc
     1081 gttttggaat ttcggatgcg gggatgtcgc cggggcgtgg ctcaagttca aagaccaaca
     1141 ggtcggcgaa gtgtgggcgg aatttttcga gaaaacaaaa gtgtccgatc aggccaagga
     1201 caccccccga ggcctcgcgg atgatcagat cgcgggcgtt gatattttct gtgatgagac
     1261 catctgtcgg gagggccgag ttgatggcgg ccatggccag actggcatca aagggctggt
     1321 ccgaaaagag tttttgggcg gcttcgtccg ttgtcagacc gggccgggaa aaaaacgcgg
     1381 aatctccgtg accgcaactc ccgatgggcc agacggtact cgcgacgccg ccatgctgcg
     1441 agagggcaaa cgagcggatt ggtccggcaa agatttgttg aatgggaaag gatggggtcg
     1501 cggcgacgag ggccttgcgc agactgtcca cgggggcatc tccttggtgt tcagccggtt
     1561 gcttgatgac cggttcagga aatgccatga caagggttgt cccannnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnttcagc cggttgcttg atgaccggtt caggaaatgc
     1741 catgacaagg gttgtcccac ggcgagtaaa agtataggac tgtccgcaaa aaaaaggcaa
     1801 gaggaatatg cagtggcgca ggaactccgc attcaataaa aatgtgctga tgtatatcgt
     1861 gacaagatca tttccacggt acgcagaccg tcgacgtatg gcgtattttc caggatccaa
     1921 tcagcccaaa gaggcagtgc ttacgtggcc tatcttaccc ttcgtctgtc tcgtccgcga
     1981 ttccgtcgga ttgccggtat aataattgta tgcggtcaac ctgacgcggg gccgccctga
     2041 tgatggcgcg gtcctcatcc gaaagttcgt acaatgtttc gaccgccgaa tccagatcag
     2101 cccacaagcg ggcaagcgca ctggtggccg ccgctttttg gccctcccct tcgacggcaa
     2161 acagggtcac ctgggccgca ctgatggcat cggcaagctg tgccagtggg ccggcatgga
     2221 cggggtcgca aagcttatgc caggggaggg gcacccggtc ggcgtactgg cggttgaatt
     2281 tatgccaacc gttatcattg agaccggttt tcagtatcgc cagagcgtta aacgtggatg
     2341 aattgaagat ggcacagacg gctttgagat ggataaggga atcagttgaa atcgaaaggg
     2401 agttaacgtt gacattgtct tgatacgttg tcccggtttc gtccaatgaa gccattgtat
     2461 cctggatagt catgggaaac aacaccttgg gccgggtctg ggaaacaaga ttttgtggtc
     2521 gtgtgtacag atgccagcga tttttttcag ggttcagttc gacattctgc gagagggctt
     2581 ttttgtgctc ttgcaggtag ttggccgcca tggggaaggc ctgctgaaag cgggtccagc
     2641 cgatttcgtc ggcctgccca ttgttgatcg tatagggaaa gatgatccag gcatgggtat
     2701 tgttgcggcg aaagggataa aatccccggt tgcggcagag cggtcgtacg gcggccagtt
     2761 cgagggtgac ttcctcgcca aggccattgg tcgcggtaac ggttgtctcc gtggcgctga
     2821 caggttgcaa ctgatagacc ttgttccaga gtgactgcaa cccgacggta atcgaaagct
     2881 cagcatgggt gccaatggtt ccaaactgga cggccagttt tttgtgcagg gccgagaggt
     2941 ccggcttgtc aagacaccag atgtcggcat ccagacttgt caggctgatt ttgcaggaca
     3001 gtgagggctt gtcgcggatg gcgtcatcaa tggaagcgta tgtccggtag cggcattgtg
     3061 ctgtgttttt tgcgaggacc agaatggcgg tataggtcgt cctgccggag aagacctgga
     3121 tgtcacgaaa atcatccacg ccctcaagca gtctgttttt ggccagaaaa ttccgcgtcg
     3181 ctgccccata gtcagtccgg aaaaagcggt tttggacgat gtatcccatc cgtccggatg
     3241 gtttgaggat cgacaccgcc ttctcgataa atggcatggc gatatcggtc tttccttcgc
     3301 gggctgtttc gtagattccg cttttgagat agtcgtacat cagcggcatc cactttttgt
     3361 aatgcttgac ctcgatatag ggtgggttgc caatcacggc gtcaaagccg ccgtttgcga
     3421 ggatggcgcc aaagccgtgg gtcgtgcttg tccagtcaaa ggggatgacc tgggagatgg
     3481 cctgcggatc aagcgcgata ttgccatcga ttccaacaag tgcgttaccg tgacgaatat
     3541 tgaggccgat accatggagc aggtcctttg gcgtttcggc ttcttcgagg gcagtgcgct
     3601 cgacacagcg caaggccagc gacatccggg cgatctcgat ggcctcgggg tcaatgtcga
     3661 caccatgcag gcatccggag atgatcgcgc gggcggttgc acggttgagc cggacttcat
     3721 ctccgtcaat gatggcccat tcgcccgctg catcccgatt ggctcggcac cagtcgacga
     3781 tcttgtcttc gagaaaggca aatgccccaa gcagaaagct gccactgccg caggaggggt
     3841 cgatgatctt gagggcaagg atgtcagcgg gcgttttccc ggttgtcaat gggtcaagca
     3901 cacgggagag gatgcggcga atcacccagt ccggagtata gacggctccc cgggtgcgct
     3961 ggtgttcggg cttgaattcg tcctcgacat cgctgccgac gaccttgaga cgtttcccga
     4021 gatagcgttc gtagatcccg cccaaaagat caatcgggat gacctcaaag cagtagggcg
     4081 agggcatgta cagccctgcg cagatggttt tgaagacgtt acccttgatc cagatatcgt
     4141 tgatgactgt ttcgactccg gtgggatcat cttcttccga atgcgggaac aaaacgccgt
     4201 cgtagacacc acgccatcgc gtttcggttt ctttcataaa tgctggccaa aatccgtctt
     4261 gatcatggac aattgccttg agggttccgt attcctccag cccgatttct tcgcaaaagc
     4321 ggataaagat gatccggtcg aggatgcgtt gggcgacagc cgaaagcagt ttggcgtcac
     4381 ggattttccc atagcgcaac acggatttgg ccagttcctt gcgccagagg gagagattgg
     4441 tttcaaagtc ggtatccagt gtcatggctc cgcgtggcag cttgtcctgc ggattgatgt
     4501 gttcgaggga tccattgata atggcctcgt gcccgaagta ttcccagagt tcatcaaagc
     4561 gggggatgta ttccttgtaa tggaaatagg cgagacgggc aaccttggca ttgtcctgag
     4621 gggccggttt gaaacggcaa tcgtagatgg ccagttcctg aaagtccatg acgtagctta
     4681 atgacatgcc agcagaccag ccataggttc tggcctggaa tgcgctggtc gaatgttcct
     4741 ggatatagat gtcaaaacgt ttggcgtcga tgtagagaat gcgctttcca ttgaccagca
     4801 gggcataatc gggtctgttg tgatgaaagc cctcagtctt gatgcgccgg gctgcgcgac
     4861 ccttgatgct gtattcgagg atgacttcag cgggatttgc gatgttccag ccgaatgctt
     4921 cgagggcttt gttgatccag atacgggctt gcgcctcgct ctgccccttg agtccggcag
     4981 tgttctcgag ttcttcaaac tcgcgtacaa ggaccgcaag cttttttttg gccaattctt
     5041 tggctgacag ggtgggtgtc acgtgggatg ccgtcctcaa gcgggataca gaaaactact
     5101 cactgactat ctgtaattcc agtagacagg aaggtgtttc aagagtgttt tcattaatga
     5161 ttaattcgat gagtgaagtg caaggtagca ggggggtggc gaagggtacg cggataatcc
     5221 gggatacgca acacaaaaac atgaatatat ggtctctatc ccggatagcc ataattgtat
     5281 tacaaaaaac agaactgatg gtttccgctt ttacccaacc cgcatcccgg tcgccagcag
     5341 gatcgggaac tccagtgtgc aggcagatcc gtccgctgca gtccggtttc gcgccatgcg
     5401 atgggcttcg atgatgcgga tattggcgag gccggcttcc gcaaaccact gcctgagctg
     5461 gtcctgatca aaaccgttat ggaagacgcc ggcattgtca tcgtgaaaga gcccaccatc
     5521 cggggccaga tcaaagatgg caagctgacc acccggcgca agccgcctgg ccaggtcagc
     5581 gatcagttgg cctgcctctt cgacgtggtg caataccatg gagaggtgga cgagatcaaa
     5641 ggggccggcg gggagaggcg aaccctgcgc gagcaggacg ggtgcgatgt ccggccgtgg
     5701 atccattgcc tcgagttttt tttcgagcgc ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnggaaagg tgcagccaga acgttgaatg tcacgacagt ttcgtcattg
     5881 ttgacatttt gttcgaattt gtacgagcag gaaccggaga ctgtccaaat cccgtcggcc
     5941 agggccagat tggtcaggct gacggtcgcg tcaatggtga tttgttggct tgagtccacg
     6001 gcgctaccgg agacgatggt tttcgactcg ctgtttgtcc aggcctttcc cgtcacgagg
     6061 gtgtttttgg ccatatcgac ttcggacttg atgacggtcc ggacgctgtc ggccagcttg
     6121 atggcatcgg cggctgagac cggcgggacg gcatcattct cgctgcaggc gacaagcgag
     6181 aacagcgcga caagcgaaac caaagaaacg atacggagag catgcatggt gaagctcctt
     6241 tcagataacc ctaccatatc aatatactgc atattttgca ttgctatccg ggataccgat
     6301 tgcaacatca cggagttatc atggttatcc cggatagcaa tgcaaaaacc gcaatatttg
     6361 tggattcatg ctccgtgcgc cctgtctctg gaggagccgg actttttggt gtatactctt
     6421 tctgaaaccc gacattcctc tgatggctgt aaaggagcct tgcatgatcc gcccactgtt
     6481 caccataagc attctttgtc ttgttgttgt gtttgccggc caggcgcagg cctccatgac
     6541 ggtgctcgat gtctaccaat ccctgcccaa ggaggcgttt gctcttgacg gaacgcagca
     6601 ccgctacgaa atcgcccaac tggggaacaa atggaagacc aaatccattg tcgggtacga
     6661 gatggatgtt gtggtcgatc tcgaaaacgg atacatcgaa atcgtggacc gcggaacggg
     6721 tggcgggttt atcaatcacg agctgtccat ctttgattcc aaaaaacggg gccgggtggt
     6781 cgccctgaac atttttggcg gggacgggac cagggccagc tcggccggga tcaagttttt
     6841 tgtgcgggag gcttcgtcct ggaaggatgt gacggccgag gttttgcctg gcatcgactg
     6901 gaccatgttt tatcaatcaa atcagtggaa catggaagac ctcaaaaaag gcgtccagat
     6961 agcggcacgg gaaaagatcg aactggtccg ttacaagatc ccgcgcatcg ggaccaccct
     7021 gcgcgccagt tttttgcggg aaaacatcat catccgcgga cagtacacga cctcgggttt
     7081 gagtccggag gaatcgcgga ttctggcatc catggtcaat acgctggcgc gctcccggat
     7141 catgctgcgc tgggacaagg aaaacggcgt ttttcgtctg cccgattgaa attggcagcc
     7201 cggtgttcct cacgcagagt ggacaccggc ccaggacagc ctcttttgac ttgcacaaga
     7261 aatatttacc ggatctggag aatacgcatg acaagtatta ttaaaaatgg catccttgtg
     7321 acggagaccc atgcccagca tggagacatc ctcctggttg acgggaagat tgcggctgtg
     7381 ggcgacctga ggaatgagtc ggcggagacg gtctatgatg cccgggggtg ttacgtcatg
     7441 ccgggcggga tcgaccccca tgtccatctc tcgctgcctg tcggaggcgg cctgctctcg
     7501 gccgatgatt ttgaaaacgg aacccgggcc gcgctggccg gcgggaccac cagcgtgatt
     7561 gattttgtca ccccggaaca tgggcaggat ctcgtgaccg ctttgcgcgc ccggcgggct
     7621 gaggcggatc accatgttct ttgcgattat gggctgcaca tgagcgtcat tgagtggcgc
     7681 gattccattc ccgaggaaat ggcccgttct gccgaactcg gagcgcccag ttttaagacc
     7741 tatctggcct ataaaaaggc catcggtctg gatgatgccg aatttgtgcg ggttttagat
     7801 gccgccgccc gcgggcggta tctcgtgaca gcgcactgcg aacacggcga cattatcgac
     7861 tatttgcaga agaactttgt tgaagaaggg aagactgcag tccgctggca ccccaggagc
     7921 cgtccggcgg cggtcgagga ggaggccgtc acccgggcca tcgcgctggc cggaacactt
     7981 ggagcttcgc tctatatcgt gcatctctcg acagcgggcg gacttgaggc ggctgcggca
     8041 gcccgtgcgg ccggacaggc actctacgtc gagacctgcc cgcattacct tcttctcaat
     8101 gaagatgcgt atgaaggcag ttttgatgaa accgcgcgct acaccatgag cccgcccctg
     8161 cgtggtccgg agcacagccg tgctctttgg gaggggattg cagccggtct ggtcgatgtc
     8221 cttgccacgg accattgccc gttttgcacc agtggacaga aggatcgcgg gcgggaggac
     8281 tt
//
LOCUS       DAOSHE010000037         8195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7484_length_8195_cov_4.122113, whole genome shotgun sequence.
ACCESSION   DAOSHE010000037 DAOSHE010000000
VERSION     DAOSHE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_8195_cov_4.122113"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="PLD82_01980"
     CDS             <1..2049
                     /locus_tag="PLD82_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG64187.1"
                     /translation="QGATHLSMAFANGMAFQASSLVGRFSVVSQFENVDLATILPGIL
                     RGQLDGKAEFKMENSIPDASWNLSVRQGSFHGTPFSVVTTGSPRQGGMQIGETRVVLG
                     DAALTVRNGHVIHTQNGFEFLFNGGFAWKGAPFQIQSRLLAQGGSDEVIVAIADIHFG
                     KTRFPDFSSVVTRSGKRILLKKQGPAGIQGWYSPEENKLDISYEDVGGMRLSLHGGFG
                     DRIDIRSEATGIPLSLLDNFPVMFRLVRGNMSASLHIQGSGEDPDISGSASIQDARFD
                     LAMLRTPLTKFSMVASLTQGTVTLGSLAATNGRGALSVRGKAFIRNGEIDDIDVRLLT
                     DRKHGLDLDLVDPEIRSSGGVLADLFISGNINAPTVQGRIALHDNEFFYMEDSAIPSR
                     ENWIKRIRWNMEVAVLDGVSYVNQLVTAMIQPGSSLQLKNSIIDKEFSVNGRVTAARG
                     TFDYINHEFRIEQPTYIEFRRTSYGLDPWLAFRGRLRIKDEELEDVDIYLTFSGPLSA
                     GINPVFSSEPERTQDEIRVLLGIEQPEKDSVDGRRKSENIMLRSTELISLLGLKPLTK
                     EIREWFGLDIFTIRTPIVRNILERESLDALDPKRQLSIFRDTQFSLGKYLTSFMFVEY
                     TLVLKDDTQNYGDVLPTHQVGLELSFDFLNLGYLIKPTEKSGFGEYEQSIELRFRQRF
                     "
     gene            2097..4514
                     /gene="bamA"
                     /locus_tag="PLD82_01985"
     CDS             2097..4514
                     /gene="bamA"
                     /locus_tag="PLD82_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOG64188.1"
                     /translation="MNCLARFRLVLVIFAMTASIPVWSAGYEDYTIIKIDLKGNVNTD
                     ASLIRALLSSKVNSKFSYRNVTRDVKDLYYLGYFSNIQVDSVIVAGGMVLTYIFEEYD
                     KIEEILFKGNDELSESDLAEVMTLRKDSVFSQIQLQKNIENILEAYRKEGFRNAKVTT
                     NVRVDKDLRKVYIRLDIDEGKKIVIQKIRILGTKELPESAVRDAIEDTHESGLFRGGI
                     YDEKVFEKDKSRIVEYYRNRGFIKMQIKKVDLRVEPVKPGSTQYGIFIDVHVEEGKRY
                     RMGRYVFEGNDIFPSDEIVTKFFRLKSGEYFDNSQYEKDQMGLWQAYRERGYIFTEIT
                     PIRQIDEQAQTVNLTYKIREKEIAHVRHIKIKGNTRSEDYVIQREMRIKEGEIFNSSA
                     IRRSQERLFNLRYFKDVNIEIDPVEGNEGLMDLVFSVKEDRTGLFTLGAGYGSVTGFT
                     FYEQISENNLFGMGLTVSQRLELGEKRKSISLGIDTPYTFTYEPVSLGFTISYSATQI
                     ENINTNYLNGPLSEAKRGDDDEYHFMRKAFEIQLRAGRSFSEWWRGYSSYTFAWIDSY
                     DANFVPLSETDATNKSSTTQEYIRRLRDALEKGYSTKSSLRLGLLYDTRDYVGGPSRG
                     IYASQFLTYTGGILGGDSQSIMSESNFSFFIPLFWDFVFAVDTKFEYILDQFDGSSNI
                     YPGDELQFDGMTELRGWRSYNQVGRGKISTMTEIRYPIDKRMLWGVFFYDAGKLWEDY
                     SKVSFNLKDFKHSFGFGFRLQIAVLPIRLYFAKKFEYDDAGHVNWIGGSGAFDGWETV
                     FSVAGVF"
     gene            4538..5077
                     /locus_tag="PLD82_01990"
     CDS             4538..5077
                     /locus_tag="PLD82_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG64189.1"
                     /translation="MKKSVILAAVVLCILPAMLPAASLKDIIKIGVVDMGKLFSEYAS
                     KSEASKSVREKKARFVKEIRDEAAVIRKMESDLKDQMAGASDSERRRRIAEIEFKKDE
                     LASLVARRNAELEKEERDITQPILQEIYEAVRFVANKQGIKIVLDSRSYIAYHDIELD
                     LTQQVILRLRLILSQKQRF"
     gene            5083..6114
                     /gene="lpxD"
                     /locus_tag="PLD82_01995"
     CDS             5083..6114
                     /gene="lpxD"
                     /locus_tag="PLD82_01995"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOG64190.1"
                     /translation="MLRGKTLTLGDLARHLQGDLRSAGDASILVRGVGPLDASGSDTI
                     TYAISPANCRIADAGSCAAVLVSSAHAEHVTKPVILVEDPKSAWIECLKLFTPRLTVP
                     KGFSPQASIDLGAMIHPDAVVMQNAVVMPGVTIGPGTVVWPGVFLGEETVVGSDCVIH
                     PNVVIREGCRLGDRVIIHAGAVIGADGFGYRQDGEGSHQKIPQIGNVVIENDVEIGAN
                     AAIDRSTCGSTVIGAGTKIDNLVQIAHNVVIGKKNILAAQVGIAGSTTTGESCFFAGQ
                     AGVADHCRIGDRVVIGPQAGVQLRRVKPDTVYFGTPAIEMGKMQKILPLFHRLPELFA
                     SRPDETREV"
     gene            6205..6654
                     /gene="fabZ"
                     /locus_tag="PLD82_02000"
     CDS             6205..6654
                     /gene="fabZ"
                     /locus_tag="PLD82_02000"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOG64191.1"
                     /translation="MSKTLDITGIMKLLPHRYPFLMVDKIVDCDEETITGLKNVTINE
                     PFFQGHFPGHPIMPGVLQVEGLAQCAGCLIFQKVENPSSYVVYFMSLNNVKFRKPVLP
                     GDTITYKVKIISFNGTRAKMQGEVMVDGKVASEAEMTAMLVQKKEEA"
     gene            6671..7471
                     /gene="lpxA"
                     /locus_tag="PLD82_02005"
     CDS             6671..7471
                     /gene="lpxA"
                     /locus_tag="PLD82_02005"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOG64192.1"
                     /translation="MIHPTAIIDSLAQIDEGVEIGPYAVLEGPVRIGRNCRIMAHAWI
                     GGDTTLGEGCVVHPFAALGGLPQDFSYNQITMHSGLQIGKNSVFREGVTVHRSAHDGG
                     ITYIGHHVYMMACSHVGHDAVIGNHVTMANSALLAGHVVVGDRAIISGNVSVHQHAHI
                     GCFAMIGASSFISQDIPPFCTVQGVPARVVCVNSIGLKRNGYDQERRFAVKRLFRLLY
                     RQGLALAAVLEKLDAIGGEDAACLAEFIRSSKRGIAAGRAPGRDHEQS"
     gene            7458..7910
                     /locus_tag="PLD82_02010"
     CDS             7458..7910
                     /locus_tag="PLD82_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HOG64193.1"
                     /translation="MSRAEIPASGVLLGIDFGLARVGLARTDALRMLASPLKILHRNG
                     KPDTFVVSTILQEVASSSAVGVAIGWPDEEDLRTEGVREAISGCIGRLEEHGIPVVRV
                     AETFSSREALELLHATGRKKKPGQFVDDWAAAVILQRYLDAADIYCRS"
     gene            7949..>8195
                     /locus_tag="PLD82_02015"
     CDS             7949..>8195
                     /locus_tag="PLD82_02015"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG64194.1"
                     /translation="MKQKTVSDQIVLQGRGLHSGQDVAVRITPAEPGSGYVFFREDLA
                     PGMPIPASWRFVQDTGHNVTLGRESVSIQTVEHLLSAL"
BASE COUNT         1975 a         1884 c         2325 g         2011 t
ORIGIN      
        1 cagggggcca cccatctttc gatggctttt gcgaatggaa tggcatttca ggccagttct
       61 ctcgttggca gattttccgt tgtctcccaa tttgaaaacg tggatctggc cacgattctt
      121 ccggggattt tgcgtgggca acttgacggc aaggccgagt tcaaaatgga aaattccatt
      181 ccggatgcgt cctggaattt atctgtccgg cagggatcat tccatgggac accattctcg
      241 gttgtgacga cgggaagtcc ccgccaaggc gggatgcaga tcggagagac ccgggttgtg
      301 ctgggtgatg ccgcactgac ggttcgcaac gggcatgtaa ttcacaccca aaacggtttc
      361 gagtttttat tcaacggggg attcgcctgg aagggtgctc ccttccagat ccagagccgg
      421 cttctggcac agggcgggag tgatgaagtc attgtggcca ttgccgatat ccattttggg
      481 aagacaaggt ttcccgattt ttcgagtgtt gtgacacggt ccggaaaacg aatccttctc
      541 aagaaacagg ggccagcagg gatccaagga tggtacagtc cggaggagaa caagcttgac
      601 atcagctatg aggatgtcgg agggatgcgg ttgtcccttc acggaggatt tggagacagg
      661 atagacatca ggagtgaagc gacaggaata ccattatccc tcctcgataa ttttccggtc
      721 atgttccgtt tagtccgcgg aaatatgagt gccagtctcc atatccaggg ctccggtgag
      781 gatcccgata tttcaggttc ggcaagcatt caggatgcaa ggtttgatct tgccatgctc
      841 cggaccccac tgacgaaatt ttcaatggtc gccagtctca cacaggggac tgtcaccttg
      901 ggctctcttg cggctaccaa cgggcggggt gctctttcag tacgggggaa agcgtttatc
      961 agaaatgggg agatcgatga cattgatgtc cgtctgctga cggacaggaa gcacggtctc
     1021 gacctcgatc tggtggatcc cgagattcgg tcctctggcg gtgttctggc cgatctgttt
     1081 atcagcggga acatcaatgc gccgacggtc caggggcgga ttgcgctcca cgacaacgaa
     1141 tttttttata tggaagattc ggccatcccc agcagagaga actggataaa gagaattcgc
     1201 tggaacatgg aagttgccgt gttggatggt gtcagctatg tcaaccagct tgtgaccgcg
     1261 atgatccagc ccggatcgtc tttgcaattg aaaaacagca tcatcgacaa ggaattttcc
     1321 gtgaatggga gggtgacggc cgcccgcggg acttttgact atatcaacca tgaattcagg
     1381 atagagcaac cgacgtatat cgaattcaga cggacatctt acggattgga tccatggctg
     1441 gccttcaggg gccgcttgcg catcaaggat gaagagcttg aggatgtgga tatctatctg
     1501 actttttccg gccccttgtc tgcaggaatc aatcccgttt tcagttctga acccgaaagg
     1561 acgcaggacg agatcagggt cctcctgggt atcgagcagc cggagaaaga ttcggttgat
     1621 ggcagacgga aaagcgagaa catcatgctc cgctcgacgg aattgatttc tctcctcgga
     1681 ctcaaaccgc ttacgaaaga aatccgggag tggttcgggt tggacatttt taccatccgc
     1741 acaccgatcg tccgaaatat cctcgaacgg gagagtctgg atgctcttga tccgaaacgg
     1801 cagctctcca tctttcgtga cacccagttt tccctgggaa agtatttgac cagtttcatg
     1861 ttcgttgaat atactttggt actgaaggac gatacacaaa actatggtga tgttttacca
     1921 acgcaccagg ttggccttga gttgagtttc gactttctca atctggggta tctcatcaag
     1981 ccaaccgaga agagcggttt cggtgagtac gaacagagca tcgagcttcg ctttcgccag
     2041 cggttctagg gataccgcgt gagggtatgt cggggtaatg atttggagcg tattcaatga
     2101 actgtctggc ccgctttcgg ctggtactgg taatttttgc catgacggca tccattccgg
     2161 tctggtctgc aggttatgaa gattacacga tcatcaaaat cgacctgaag ggcaatgtca
     2221 ataccgatgc ctcgttgata cgtgctctgc tttcaagcaa ggtcaattcg aaattttcgt
     2281 acaggaatgt gacgcgggac gtcaaggact tgtactatct gggctatttc tccaatattc
     2341 aggtcgattc ggtcattgtt gccggcggaa tggttctgac ctacatcttc gaagagtatg
     2401 ataaaataga ggaaatcctt ttcaagggaa acgacgagct ctcggaaagc gatcttgctg
     2461 aggtgatgac tctgcgcaag gattcggtgt tcagccagat ccagctccaa aaaaatatcg
     2521 agaacattct tgaagcatac cgcaaggaag gcttcaggaa tgccaaagtc acgacgaatg
     2581 tcagagtgga caaggatctg cgcaaggtct atatccggct tgatatcgac gaaggaaaaa
     2641 aaatcgtcat tcaaaaaatt cgtattctcg ggacaaagga attgccggag agtgcggtgc
     2701 gtgatgccat cgaggatacc catgaatccg ggttgttccg cggcggcatc tatgacgaaa
     2761 aagtctttga aaaagacaaa tcgagaatcg tcgagtatta caggaatcgc ggtttcatca
     2821 agatgcagat caaaaaagtc gatctccggg tcgagccggt caagcccgga tccacccaat
     2881 acgggatttt catcgatgtg catgttgaag agggaaagcg gtaccggatg gggcgctatg
     2941 tctttgaagg gaatgacatt ttcccgagtg atgaaattgt cacaaagttc ttccgattga
     3001 agtccgggga gtatttcgac aacagtcagt acgaaaaaga tcagatgggg ctctggcagg
     3061 catatcgaga gcgaggatat attttcaccg aaatcacccc catccggcag attgacgaac
     3121 aggcgcagac tgtcaatttg acctacaaga tccgggagaa agagatcgcc cacgttcgac
     3181 acatcaagat caaggggaac acgcgaagtg aagattacgt gatccagcgc gagatgcgaa
     3241 tcaaggaagg cgagatcttc aacagttccg cgatcaggcg ttcccaggag agattgttca
     3301 acctgcgcta tttcaaggat gtcaatatcg aaatcgatcc cgttgagggc aacgagggtc
     3361 tgatggatct tgtgttctcg gtcaaggaag accggaccgg actttttacg cttggagccg
     3421 ggtatggctc tgtgaccggt ttcacgtttt atgaacagat ctccgagaac aatcttttcg
     3481 gaatggggtt gacggtttcg caaagacttg aactcggtga gaaacgcaag agcatttctt
     3541 tggggatcga tacaccgtat acgttcacct atgaaccggt ctcgctcggg tttaccattt
     3601 cgtacagtgc cacccagatt gaaaatatca atacaaacta tttgaatgga ccgctttctg
     3661 aagccaaacg cggagatgat gacgagtacc atttcatgcg aaaggctttc gagatccagc
     3721 ttcgtgccgg gcggagtttc tccgaatggt ggcgtgggta ttcttcctat acattcgcct
     3781 ggattgacag ttacgacgcc aattttgtcc cgctcagtga aacggatgcg acgaacaaga
     3841 gttcgaccac acaggaatat atccgtcgct tgcgggatgc cctcgaaaag gggtacagca
     3901 ccaagagcag tctgagactc ggtcttctgt atgacacccg tgactatgtc ggcggtcctt
     3961 cgcgcggtat ctatgccagc cagttcctga catatacagg aggcattctc ggtggagaca
     4021 gccagtcaat catgtccgag agcaattttt cgttcttcat ccccctcttc tgggacttcg
     4081 tgtttgcagt ggataccaag ttcgagtaca tccttgacca gttcgatgga tcttcaaaca
     4141 tatatccggg tgatgaactc caatttgacg gtatgaccga attgcgcgga tggcgaagtt
     4201 acaaccaggt cggacggggc aaaatttcga ccatgacgga aattcggtac ccgattgaca
     4261 agaggatgct ctggggtgtg tttttctatg atgccgggaa gctttgggag gattacagca
     4321 aggtttcctt caatctcaag gacttcaagc acagcttcgg attcgggttc cggctccaga
     4381 tcgccgtttt gccgatcaga ctgtattttg cgaagaaatt cgagtatgat gatgctggcc
     4441 atgtgaactg gattggtggt tcaggcgctt ttgatggatg ggaaaccgtc ttttcagtgg
     4501 caggtgtttt ttgaccagat atgttttgga ggataccatg aagaagtctg tcattctcgc
     4561 ggcggttgtg ctctgcattc tgccggcaat gctgcccgct gcctcgctca aggatatcat
     4621 caagatcggt gttgtcgata tgggtaagct cttttcggaa tatgcatcca agagcgaagc
     4681 ttcaaagtcg gttcgggaga aaaaggccag atttgtcaaa gagatccggg atgaggccgc
     4741 tgtgatcagg aagatggaaa gcgatctcaa ggatcagatg gccggtgcgt cggacagcga
     4801 gagacgcaga cggattgcag aaattgaatt caagaaggac gaactggcaa gcctggttgc
     4861 aaggcggaat gccgagctgg aaaaagagga gcgggatatc acgcagccga tccttcagga
     4921 aatctatgag gcggtcaggt ttgtggccaa caagcaggga atcaagatcg ttctcgacag
     4981 tcgttcatac atcgcctatc atgacatcga actggatttg acacagcagg tgattcttcg
     5041 attgcgcttg atactttcgc aaaaacagcg gttttaagaa ccatgttgcg gggtaagacc
     5101 ctgacgctgg gtgatttggc caggcatctc cagggagatt tgcgctcggc cggtgatgcg
     5161 tccattctgg tgcggggtgt cggtccgctg gacgcctcag gatctgatac gattacgtat
     5221 gccatttcgc cggccaattg ccgaattgcg gatgccggat cctgtgccgc cgtccttgtg
     5281 agcagtgccc atgcggaaca cgtcacgaaa cccgtcattc ttgtcgagga tccgaaatcc
     5341 gcctggatag aatgcctgaa attgttcacc ccccgactga cagtgccaaa aggattttca
     5401 ccacaggcat ccattgatct tggtgccatg atccatcctg atgccgtggt catgcaaaat
     5461 gcggttgtca tgccgggagt cacgatcggc ccgggtacgg tcgtctggcc gggtgtcttt
     5521 ttgggtgaag aaaccgttgt tggttccgat tgtgtgattc atcccaatgt tgtcatccgt
     5581 gaaggatgcc gactcggaga cagggtcatc atccatgccg gggccgtcat cggtgcagac
     5641 ggattcgggt acagacagga cggggaaggt tcccaccaaa aaattccgca gatcggaaat
     5701 gttgtcatcg aaaatgatgt cgagattggt gccaatgctg ctatcgacag gtcgacgtgc
     5761 ggttcaaccg tgatcggagc cggcaccaag atcgacaact tggtccagat cgcccacaat
     5821 gttgttattg gcaaaaaaaa tattcttgca gctcaggtcg gtatcgccgg gagtacgact
     5881 actggtgaga gttgtttttt tgccgggcaa gcgggggtgg cagaccattg ccggatcggt
     5941 gatcgtgttg tgattggccc tcaggctgga gtccagctca ggagggtcaa accggacacc
     6001 gtctattttg ggacaccggc tattgaaatg ggcaagatgc agaaaatatt accattgttt
     6061 cacagactcc ccgaactttt tgcaagcagg ccagatgaaa cccgagaggt atgaatagct
     6121 tgtgtgtact ggacgtttca ggagtttctg atgatacttg agagaacata ttcatcgaga
     6181 atgcacctat aacaggagga gaagatgtca aagacgctgg atataaccgg gatcatgaaa
     6241 ctgcttccac accgttatcc ttttttgatg gtcgacaaga tcgtggattg cgacgaagaa
     6301 acgatcacag ggctcaagaa tgtcacaatc aatgagccat tcttccaggg gcatttcccc
     6361 ggacatccga tcatgccggg tgtgctgcag gtcgagggtc tggctcaatg tgctggatgt
     6421 ctgatttttc aaaaggttga aaatccgtca tcctatgttg tgtacttcat gagcctgaac
     6481 aacgtgaaat tcagaaagcc ggttttgccc ggtgatacca ttacctacaa ggtcaagatc
     6541 atcagcttca acgggacaag ggcgaagatg cagggtgaag tcatggttga tggcaaggtt
     6601 gccagtgaag ctgaaatgac ggccatgctg gtacaaaaaa aagaagaagc ttgatactta
     6661 tggagcaggt atgattcatc ccactgcaat aatagacagt ttagcacaga tcgatgaagg
     6721 ggttgagatt ggcccgtacg ctgtactgga agggcctgtg cgaattggcc ggaattgcag
     6781 gatcatggct catgcctgga ttggcggtga cacgactctt ggggaggggt gtgttgtaca
     6841 cccctttgcc gctctcggcg gtctgccaca ggatttttcg tataatcaga tcacaatgca
     6901 ttccggactc cagatcggca agaattcggt tttccgggaa ggtgtgacgg tgcaccgcag
     6961 tgcccatgat ggcgggataa cctatatagg ccatcatgtc tacatgatgg cctgcagcca
     7021 tgtcgggcat gacgctgtaa ttggcaatca cgtcaccatg gcgaattccg cgcttctggc
     7081 agggcatgtc gtcgtcggag accgggcgat tatttccggc aatgtctccg ttcatcagca
     7141 tgcacatatc ggctgttttg ccatgatcgg agcatcgagt ttcatcagtc aggatattcc
     7201 tcctttttgc acggtccaag gtgttccggc ccgggtcgtg tgtgtcaatt cgatcggtct
     7261 caagcggaat ggatatgatc aggaacggcg ctttgcagtc aagagattgt tccgcctgct
     7321 ctatcgacaa gggctggctc ttgcggccgt tttggaaaaa ctcgatgcca tcgggggcga
     7381 ggatgcggcc tgtcttgcgg aatttatccg ttcctccaaa cgcggcattg ctgccggacg
     7441 tgccccggga agagatcatg agcagagctg agattcccgc atcaggtgtt ctgctcggaa
     7501 tcgattttgg tttggcacga gtcggtcttg ccagaaccga tgcgctccgg atgctagcct
     7561 cgccactcaa gattcttcac agaaacggga aacccgatac gtttgtggtg agtacaattc
     7621 tgcaggaggt cgcatcttcc tcggctgttg gcgttgcgat tggatggccg gatgaggagg
     7681 atctccggac agaaggtgtc cgggaagcga tatccggttg tattggccgt ttggaggaac
     7741 acggtatccc ggttgtccgt gttgccgaaa ctttttcaag ccgtgaggcg ctggaattgc
     7801 tgcatgccac cgggaggaaa aaaaaacctg gtcaattcgt ggacgattgg gctgcggccg
     7861 tgattttaca acggtatctt gatgctgcgg atatctattg tagatcgtga tacattcgta
     7921 ctggcgtgaa ggattccggt ggggagcgat gaagcagaag acggtttcag atcagatcgt
     7981 gcttcaaggc agagggttgc atagcggcca ggatgttgct gtgcggatca cgccggccga
     8041 acccgggagc gggtatgttt ttttccgtga ggatctcgca ccgggcatgc ccataccggc
     8101 atcgtggcgg tttgttcagg atacgggcca caatgttacc ttggggagag aaagcgtctc
     8161 catccagaca gtggaacatc tcttgtccgc ccttg
//
LOCUS       DAOSHE010000038         8194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7487_length_8194_cov_3.717041, whole genome shotgun sequence.
ACCESSION   DAOSHE010000038 DAOSHE010000000
VERSION     DAOSHE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7487_length_8194_cov_3.717041"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="nusA"
                     /locus_tag="PLD82_02020"
     CDS             complement(<1..1439)
                     /gene="nusA"
                     /locus_tag="PLD82_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG64195.1"
                     /translation="MGSNFADAVRQLIHEKGIPSELVYGAVEKGLTAAYKRKYQTDEN
                     VVVRFDDETGDILMFARKEIVEEIDNPVFDIDLESARALETEADIGDEILVSLPVDEF
                     SRIAAQSARQIISQELRKIERNIIYNEYIHRKGEIINGYVQREKNGMYFINLGKTEGI
                     MPRSHQSPREKFQIGERIKAYLYSVEEDDRGTRIVLSRGCPEFVRHLFEVEVPEVYDN
                     IVQIKGIVREAGYRTKVAVYSSDGAIDPIGTCVGVKGVRIQTIIREINGEKIDILRYS
                     HDTREFIRNAMSPVKELRVFLLSEARKEALVVVPDREDDTQFSLAIGKNGLNVKLASK
                     LTGWNIDVRKESEMEGVEIVEESMQRAHSLFRNDGEEELDEAAYEEEEIWDEGAVEED
                     EGTPLDEIEDFDNDIIGTLEQNGVRTVEQLVELGRDELLAMEGIDEALADTILRIMQE
                     SVTYLDDGEEENIAEGDVVYECTHCGHKVR"
     gene            complement(1576..2472)
                     /locus_tag="PLD82_02025"
     CDS             complement(1576..2472)
                     /locus_tag="PLD82_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG64196.1"
                     /translation="MQTDSWRLTGGMLAALSLLMLGFTGVYRGESRDDEPRMQPRNQI
                     VRIERENPEERQPARRIVPLHSLDQRSAPLNQETPYIATKENPWSGQTAEQTREWSPA
                     KPDAGLQDDRPRAEKQSEERDGIETRPEESPLFPFEAGMFTRATVQQMMQTEKILSPF
                     QELPQALRPDARLLVHFQSRGQVSLGTMAEKNAEYFHAMVRKISGQWGIHFPRFQHFY
                     GLLKEGEVYLVFELDLDGNVTSARIVQSYGQPSIDDSCLRAIQGAANFGPIPVEYRDK
                     GKMIIPFVFVYHRPDQPMKMFH"
     gene            complement(2528..3121)
                     /locus_tag="PLD82_02030"
     CDS             complement(2528..3121)
                     /locus_tag="PLD82_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOG64197.1"
                     /translation="MGRPRLSRLLAAVLIVILVTGLGLILTSEYVRTTGNAILDGKDR
                     FTEKPVAIVLGAAVWPGDVPSHMLEDRVRTAVRLLKEGRVSCLLMSGNREPGYDEPAA
                     MRTLAQKLGVPASDILEDGRGWTTRHTMQNAAKNFSIRYAIIVTQSYHLPRAVFLARE
                     AGLVAQGVPADLREYRDQSSNEIREFFARVKAWLQEL"
     gene            complement(3114..4418)
                     /gene="hisS"
                     /locus_tag="PLD82_02035"
     CDS             complement(3114..4418)
                     /gene="hisS"
                     /locus_tag="PLD82_02035"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG64198.1"
                     /translation="MGAIQRIKGTNDILPDHLGSEIFRSGLWRKLEETSFRIAGLSGY
                     QEIRTPIFESTELFVRGLGDVTDIVKKEMFSFTDRGERQLTLRPEGTASIVRAWLENG
                     IGVADPVARLSYSGPMFRAERPQKGRYRQFHQFGVECIGGPDVRHDIEVIALFWNILT
                     ALGVRGVKLLVNSVGCPGCIPHYRDVLRAHFMPHLGDLCEDCRERHQSNVLRMLDCKN
                     QSCQEILSTAPAVTDHLCEVCARSWEGLQDGLGMAGIPWDHDPRLVRGLDYYTKTAFE
                     IVHTAIGAQGTIVGGGRYDGLIAASGGPAVPAVGGAFGIERMLLAMQAEGVADPSPVR
                     PRYFLVQIGDGVDEGMERLLHGLRAAGISVLRADGSRQMGKQMRAASKAGSQEAVFIG
                     GDEWDSGKLAIKDLDSGNQEVIEPGSDGPLAALVRRAGTGNG"
     gene            complement(4432..5052)
                     /locus_tag="PLD82_02040"
     CDS             complement(4432..5052)
                     /locus_tag="PLD82_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG64199.1"
                     /translation="MRIERFVVGSIETNAWLIESEGEAFLVDPGAEDQALLTAASQAG
                     LVGVFLTHGHVDHIAGLRPFIGQGVRVFIGGLDAAALYDPRVNLSVWMGQPFSVPECV
                     PQTLTGGETLVFGEMHCQVLHLPGHSPGSIGLLVNGHLFSGDVLFQSSIGRTDLPGGD
                     FQTLQQSIRETLYRLPDGTIVHPGHGDDTTIRAEKNDNQYCRIEKG"
     gene            complement(5052..5504)
                     /gene="dtd"
                     /locus_tag="PLD82_02045"
     CDS             complement(5052..5504)
                     /gene="dtd"
                     /locus_tag="PLD82_02045"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG64200.1"
                     /translation="MRAVVQRVKQASVRVHAEQIAAIGSGALVLLGVGKNDSEDDAQY
                     LAEKITGLRIFPDEHADMNLALSEVSDHAVLVVSQFTLFGDCRKGRRPSFTNAASPER
                     AEELYYSFIQALQAMGVRVACGRFRSMMEVSLVNDGPVTILLDSKKEF"
     gene            complement(5501..6685)
                     /locus_tag="PLD82_02050"
     CDS             complement(5501..6685)
                     /locus_tag="PLD82_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64201.1"
                     /translation="MAEETRQEDAKALMQTGKRLLAARRFADAWEVFDQALRKDKRDP
                     RIHTLLGIASLYQEFLDDAREYFEKAIELDRDNADARCGLSYVMMRQDRVAEATEELC
                     DVLRIHPRHVIAKKRFAELKKAESIDAWIAGLHPSMFVELPKSQREIRLPGWANASKS
                     RQVAIWVMLVCMLVLLGFCAANGLYIQKRRGGTPGPQATDRYYPAAVPLDTDIGQRLQ
                     RAMRAAQKPEEVFLSDSEVSSLLTRAQALLRNGEYNEARHLVNKLLASNADRVSLDMA
                     KRLDAFVKAPQADRLHYNPELDDVIEHGPLFKGVHVKWLTRVVRRTNQVFLQVAPALA
                     HRKDDKSTRVVVLDRTAERYNPGATVEIFGEVVGVDTADQEDRVIFIEGKKMQRLERG
                     IR"
     gene            complement(6678..6908)
                     /locus_tag="PLD82_02055"
     CDS             complement(6678..6908)
                     /locus_tag="PLD82_02055"
                     /inference="COORDINATES: protein motif:HMM:NF021542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOG64202.1"
                     /translation="MAGFGEWIKNVFDESPAQMAGAVAGFVCAILVITIGFWNTMLVV
                     FFTLAGIFGGCLLEHGGDVKACLERFRRRRNG"
     gene            complement(7059..>8194)
                     /locus_tag="PLD82_02060"
     CDS             complement(7059..>8194)
                     /locus_tag="PLD82_02060"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOG64203.1"
                     /translation="ITGADAVHPGYGFLAENSKFAEICADHGIIFIGPKPATIDMMGD
                     KATARRTALAAGVPITPGTDIIHDVEKGLAEAKKIGYPVIIKATAGGGGKGMRVAQDE
                     DEFRRSYEIAKNEAGKAFGNPDVYVEKFITEPHHVEIQVMADAHGNVVHLGERDCSIQ
                     RRHQKLIEESPSPLMTSELRKKMGEAAVKCAKAANYLGAGTVEFLVDDKRNFYFMEMN
                     TRIQVEHPVTEMLTGVDLLQEQIRVAAGEKLSVRQEEIRFVGHVIECRINAENPYKDF
                     IPCPGKITGLHVPGGFGVRVDSHIYEEYEIPPYYDSLVAKLIVHGRDREQAIRRMLRC
                     LDEFIIEGIRTTIPIHKQILSHERFVKGDFTTKFLEEVTFTGE"
BASE COUNT         1786 a         2494 c         2089 g         1825 t
ORIGIN      
        1 cgcaccttgt gcccgcaatg cgtacactca tacacaacat ccccctcggc gatattctct
       61 tcctcgccat catcgagata ggtgacgctc tcctgcatga tgcggaggat cgtatccgcc
      121 agagcttcat caatcccttc catggcaagg agttcatccc gtccaagctc gacaagctgc
      181 tcaaccgtcc gcacaccgtt ttgctccagt gtaccaatga tatcattatc gaagtcttcg
      241 atttcgtcaa ggggtgtgcc ttcatcctct tcgacagcgc cctcatccca gatctcttct
      301 tcctcgtacg ccgcctcgtc aagctcttcc tctccatcat tcctgaagag gctgtgggcc
      361 cgctgcatcg actcctcgac aatctcgacg ccttccatct cgctctcctt gcggacatcg
      421 atattccacc cggtaagttt tgatgccagt ttgacattca aaccgttttt gccgatggca
      481 agcgagaact gggtatcatc ctccctgtcc ggaacaacaa cgagtgcctc ttttctggct
      541 tcgctgagga ggaacaccct caattccttg acggggctca tggcgttgcg gataaattcg
      601 cgggtatcgt ggctgtacct gagaatgtcg atcttttccc cgttgatctc ccggataatt
      661 gtctggatcc tgacgccctt gacgccgacg caggttccga tcggatcaat ggccccgtca
      721 gacgaataga cagcgacctt ggtcctgtaa ccggcttccc ggacaatccc cttgatctgg
      781 acaatattgt cgtagacctc aggaacctcg acttcaaaga ggtggcggac aaactcggga
      841 cacccgcggg acaggacaat cctggttccg cggtcatctt cttcgacact gtagagatac
      901 gccttgatgc gttcaccaat ctggaatttt tcgcggggag actggtgact cctgggcatg
      961 atgccctcgg tcttgccgag attgataaag tacatcccgt ttttctcgcg ctggacatat
     1021 ccattaatga tctcgccctt gcgatggatg tactcgttat agatgatatt gcgctcgatc
     1081 ttccgcaatt cctgcgagat gatctgccgt gcggattggg ctgcaattcg actgaattca
     1141 tcgacgggaa gggaaaccag gatctcgtct ccgatgtccg cttcggtttc aagtgcccgt
     1201 gcagattcca gatcgatatc aaaaaccgga ttgtcgatct cttcaacaat ttccttccgt
     1261 gcgaacatga gaatatcgcc ggtctcatca tcaaacctga ccacaacatt ttcgtcagtc
     1321 tgatacttgc gcttgtaggc agccgtcaaa cccttttcga cagcaccata caccagctcg
     1381 ctggggatac ccttttcgtg tatcagttgc ctgacggcat ccgcaaaatt ggaacccatg
     1441 tgggcactcc ttgaatgttt tgaagcaaaa aaaagagccc ccggcagcgg gatgcccttt
     1501 cgcgtatgca aaattgctct ttcatcaatc taacagagag atgtggccct gtcaagatgt
     1561 ttttctcgta gtgtttcaat gaaacatttt catcggttga tccgggcgat gatagacaaa
     1621 aacaaaggga atgatcatct tgcccttgtc gcgatactcc accggaatgg gaccgaaatt
     1681 ggctgcccct tgaatggccc tcaaacagga atcgtcgatg gaaggctgtc catatgactg
     1741 gacaatccgg gctgaagtca cattcccgtc aagatcgagt tcaaagacca gatagacctc
     1801 cccttctttg agaagcccgt aaaagtgctg aaaccgggga aagtggatcc cccattgtcc
     1861 ggaaatcttg cggaccatgg catggaagta ttcggcattc ttttcggcca tcgtccccag
     1921 actgacctgc ccgcgcgact gaaaatggac cagcaatcgt gcatccggac gcagagcctg
     1981 cggaagttcc tggaacggcg agagaatctt ctcggtctgc atcatctgct ggactgtcgc
     2041 acgcgtgaac atccccgctt cgaatggaaa gagcgggctc tcttccggcc tggtctcgat
     2101 cccgtcacgt tcctcactct gtttctctgc gcggggtcgg tcatcctgca gaccggcatc
     2161 cggtttggca ggagaccact cccgtgtctg ttccgcagtc tgtccggacc agggattctc
     2221 ctttgtggcg atatatggcg tttcctggtt caaaggtgcg ctgcgctgat caagggaatg
     2281 gagcgggacg attcgccttg ccggctgccg ttcctccgga ttctcccgct cgatccggac
     2341 aatctggttt cttggttgca tccggggttc atcatcccgt gattcaccac gatagacacc
     2401 ggtaaagccc agcataagca atgaaagcgc tgcgagcatc ccgcccgtca aacgccagga
     2461 gtctgtctgc acctgcaaac tcctttccgt atcccgtacc caaagtatag cacaggaccg
     2521 gaaccggtca caactcctgc aaccatgctt tgacgcgggc aaaaaactcc cggatctcgt
     2581 tcgaggactg gtctcgatac tccctgagat ccgccggcac cccttgtgca accagtccgg
     2641 cttcacgggc aagaaagacg gccctgggga gatggtagga ctgggtcaca atgatcgcat
     2701 agcggattga aaaattcttt gccgcattct gcatcgtgtg ccgggtcgtc cagcccctgc
     2761 catcctcaag aatgtcggac gcagggacac cgagcttttg ggcaagtgtc cgcatggcag
     2821 ccggctcgtc ataccctggc tcccggttcc cggacatgag gagacaggag accctaccct
     2881 ccttcaaaag ccggacagcc gtccgtacac ggtcttcaag catgtgcgag ggaacatccc
     2941 ccggccagac agccgcaccc agaacaatgg caaccggttt ttcggtaaaa cgatccttcc
     3001 cgtccaggat cgcgttaccc gtcgtacgga cgtattcact ggtcaggatg agcccgagcc
     3061 ccgtgacgag aatcacgata aggacggctg caaggagtct tgacaagcgt ggcctaccca
     3121 ttccccgtcc cggcccggcg gacaagagcg gcgagaggtc catcggaacc gggctcgatc
     3181 acctcctggt ttccgctgtc cagatccttg atggccagct ttccggaatc ccattcatcc
     3241 ccgccgataa acacggcttc ctggctccct gccttggatg ccgcccgcat ctgcttcccc
     3301 atctgtctgc tgccatcggc ccggaggacc gagatcccgg ccgcgcgcaa tccgtgcagc
     3361 aagcgctcca tcccttcatc gacaccgtcg ccgatctgga ccagaaaata acggggcctg
     3421 accggagatg gatccgcaac gccctcggcc tgcatcgcga gcagcatccg ttcaatcccg
     3481 aaagcgccac cgacagcggg aacagccgga cctccactgg ccgcaatcaa gccgtcatag
     3541 cgaccgccac cgacaattgt cccctgcgca ccgatcgctg tatgtacaat ttcaaaagct
     3601 gtttttgtat aataatccag accccggaca agacggggat catggtccca gggaataccg
     3661 gccatcccga gcccgtcttg caagccttcc caggaacggg cacacacttc gcagagatga
     3721 tccgtcaccg cgggggccgt cgagagaatc tcctggcagg actggttctt gcagtccagc
     3781 atccgcagga cattgctctg gtgccgttcc cggcagtctt cgcagagatc cccgaggtgt
     3841 ggcataaagt gggcacgcaa cacatcacga tagtgcggaa tgcagccggg acaaccgaca
     3901 ctgttgacga ggagtttgac accgcgcaca cccaatgccg tgaggatgtt ccaaaaaagc
     3961 gctatcacct cgatgtcatg ccggacatcc ggaccgccaa tgcactcgac tccgaactgg
     4021 tggaactgtc tgtaccgccc cttctgtggc cgttctgccc tgaacatcgg accggagtag
     4081 gaaaggcgcg ccaccggatc ggcgaccccg atcccgtttt cgagccaggc ccggacaatg
     4141 gacgccgtgc cttccggccg aagcgtcaac tgccgttcgc cccggtcggt aaaactgaac
     4201 atttcttttt tgacaatgtc tgtcacatca cccaagccgc gcacaaagag ctcggtcgat
     4261 tcaaaaatcg gtgtccggat ctcttgatag ccggagagac cggcaatcct gaaagatgtt
     4321 tcttccagct ttctccagag acctgacctg aatatttcgc tgccaagatg atccggaaga
     4381 atgtcgtttg tccctttgat tctctgaatc gctcccatac cccgccttcc ttcaaccctt
     4441 ctctattctg caatattgat tatcgttttt ctcggcccgg atcgtcgtgt cgtcaccgtg
     4501 acccggatgg acaatggtcc cgtccggcag gcgatagagg gtctcccgga tgctttgttg
     4561 aagagtctga aaatcgccac cgggcagatc agtccgacct atcgaagact ggaagagaac
     4621 gtccccggaa aaaagatggc cattcacaag gagtccgata cttcccggag aatgtcctgg
     4681 aagatgcaga acctgacaat gcatttcacc gaatacaagt gtctcccccc ctgtcagtgt
     4741 ttgtggaacg cactccggca ccgaaaaggg ctggcccatc cagacagaga gattgacccg
     4801 cggatcgtag agtgccgctg catccaagcc acctatgaaa acgcggacac cttgcccgat
     4861 gaaaggacga agacccgcga tatggtcaac atgcccgtgt gtcaggaaaa caccgaccaa
     4921 tcccgcttgt gaggcagcgg tcagcaaggc ctgatcctcg gcaccgggat cgacaaggaa
     4981 agcctctccc tcactctcaa tgagccaggc attggtctcg atgcttccga cgacaaaacg
     5041 ctcgattctc atcagaattc ctttttgctg tccagcagga ttgtcaccgg cccgtcattg
     5101 acgagggaga cttccatcat cgaacggaac ctcccgcaag cgacgcggac ccccatggcc
     5161 tgcaacgcct gaataaagga gtaatacagt tcttctgccc gctccgggga tgccgcgttg
     5221 gtaaaggacg gtcgccggcc cttgcgacaa tcaccgaaca gggtaaactg ggagaccaca
     5281 agcacggcat ggtcggagac ttcggagagt gcgaggttca tatcggcatg ctcatcggga
     5341 aaaatacgca gacctgtgat cttttcggca aggtactggg catcgtcctc ggaatcgttc
     5401 ttacccacgc ccagcaggac cagtgccccg ctcccgatgg ccgcgatctg ttcggcatgg
     5461 accctgaccg aggcctgctt gacccgttgg acaaccgccc tcaccggatg cctctctcaa
     5521 gccgctgcat tttctttccc tcgataaaaa tcactctgtc ttcctggtcc gctgtatcga
     5581 caccgacgac ctctccaaag atctcgactg tcgccccggg gttgtaccgt tcggcagtcc
     5641 tgtcaagaac gacgacacgg gtactcttgt catctttacg gtgcgccagt gccggggcca
     5701 cctgcaggaa gacctgattg gtccgccgga caacccgggt cagccatttg acatgaacac
     5761 ccttgaagag gggaccatgc tcaatcacgt catccagttc aggattgtaa tgcaaccggt
     5821 ccgcctgcgg agctttgaca aaggcatcca gtcttttggc catgtcaagc gagacccggt
     5881 cggcattgga tgccaagagt ttgttgacca gatgccgggc ctcattgtac tccccgttgc
     5941 gaaggagtgc ctgggcccgg gtcaacaggg aagagacctc ggagtcggag aggaaaacct
     6001 cttcgggctt ctgggcggcc cgcatggccc gttggagccg ctgcccgata tccgtgtcca
     6061 gcggtacggc tgccggataa tagcgatccg ttgcctgtgg cccgggggtc ccacccctgc
     6121 gtttctggat atacaatcca tttgctgcac aaaatcccag caacaccagc atgcagacca
     6181 gcatcaccca gatagcaacc tggcgggatt tggaagcatt ggcccatccc ggaagcctga
     6241 tctcacgctg cgacttggga agttcgacaa acatcgaggg atgaagaccg gcgatccagg
     6301 catcgatcga ctccgccttt tttaattctg caaaacgttt tttggcgatt acatggcggg
     6361 gatggatccg caacacatca cagagttcct cggtggcctc ggcgaccctg tcctgtcgca
     6421 tcatgacata cgaaagacca caccgggcat ccgcgttgtc acggtccaat tcgatcgcct
     6481 tctcgaaata ctcacgggcg tcatccagga actcctggta gaggctggct atccccaaga
     6541 gagtatggat ccggggatcc cttttatcct tgcgcaatgc ctggtcaaag acttcccatg
     6601 cgtcagcgaa ccgacgcgca gccaaaagac gtttccccgt ttgcatcagg gctttcgcat
     6661 cttcctgtct ggtctcttca gccattgcgc cttctcctga atcgttcaag acatgcctta
     6721 acatcccctc catgctcgag gagacaaccg ccaaaaatac cggcaagagt aaagaagacg
     6781 acaagcatgg tattccagaa ccctatggtg atcacgagga tggcacagac aaagccggcc
     6841 acggcccccg ccatctgggc tggtgactca tcgaagacat ttttaatcca ctctccgaac
     6901 cccgccatac ctgcctcact tgttccgtcg cttgtaaaaa aactatcagg cagacctgcc
     6961 ccgggtcaag cccggacctg atacagaaaa acgccatcat cctctccgac gatggcgttt
     7021 ttcatggcac accggaagct ggccgcattt tgagggactc actccccggt aaacgtcact
     7081 tcctcgagaa attttgtcgt gaaatcaccc ttgacaaagc gttcatggga aagaatctgt
     7141 ttgtggatgg gtatcgtggt gcgtatccct tcgataatga actcgtcgag acagcgcagc
     7201 atccgcctga tcgcctgctc acggtcccgg ccatgcacga tcagcttggc aacgagagaa
     7261 tcgtaatacg gtggtatctc gtattcctca tagatgtggg aatcaacgcg caccccgaaa
     7321 ccgccgggaa cgtgcagtcc ggtgatcttt cccgggcatg gaataaaatc cttataggga
     7381 ttttctgcat tgatccggca ttcgatgaca tgtccgacaa acctgatctc ttcctgcctg
     7441 accgagagtt tttctccggc tgccacacgg atctgctcct gaaggaggtc cacacccgtc
     7501 aacatctcgg tgacagggtg ctcgacctgg atccgggtat tcatttccat gaagtaaaaa
     7561 ttgcgcttgt cgtcaaccag gaattcaacg gtccctgcgc caagatagtt ggcggccttg
     7621 gcacacttga cggcggcctc acccatcttc ttgcgcagct cggacgtcat caggggagaa
     7681 ggactctctt cgatcagctt ctggtgccgc ctttggatgg aacaatcccg ttctccgaga
     7741 tggacgacat tgccatgggc atccgccatc acctggatct cgacgtggtg cggttcagtg
     7801 atgaattttt cgacatagac atccggattt ccgaaagcct tgccggcttc gttcttcgca
     7861 atctcgtagg atctgcggaa ttcgtcctcg tcttgcgcga cacgcatccc cttcccgccg
     7921 ccgccagccg tggccttgat gatcacgggg tacccgattt ttttggcttc ggccagaccc
     7981 ttctccacat catgaatgat gtcggtcccg ggtgtgatgg gaaccccggc ggccagggcc
     8041 gtccgtctcg cggttgcctt gtctcccatc atgtcaatcg ttgcgggttt tggaccgata
     8101 aagatgatcc cgtgatcggc gcagatctcg gcgaatttgg aattctccgc caggaatccg
     8161 tagccgggat ggacagcatc cgcacccgtg atct
//
LOCUS       DAOSHE010000039         8102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7617_length_8102_cov_4.328818, whole genome shotgun sequence.
ACCESSION   DAOSHE010000039 DAOSHE010000000
VERSION     DAOSHE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_8102_cov_4.328818"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLD82_02065"
     CDS             <1..406
                     /locus_tag="PLD82_02065"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG64204.1"
                     /translation="TLMFADLRYARLVPVTREVPREHWHRDALKTLVQALVDGPVNND
                     TLPVVPAETRILGCWQDGKTAWIDFDRNIFLGLADAADAEVLCAYGIVNTIIKNLPDI
                     ERVQILVEGAPRQTLRGLTRVVQPLEPRTDLE"
     gene            560..1627
                     /locus_tag="PLD82_02070"
     CDS             560..1627
                     /locus_tag="PLD82_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOG64205.1"
                     /translation="MRKATRIAGIVLLAVCMFLMTLAYAQTVDATGSQSGRLVANRAF
                     VIDFGQYESRMKAQIERNKKLIESRTNASAARGLPTEKFTMEASDWALDKWEVELNSS
                     ASSIMNNVLSYVVKVGSPWWLNNAKRIYSGMAGKLEGYNETPASNVLGVRIHFPTHRQ
                     NCWAKIHPPFEINAYDDKGGLANTNNGIVDNVGQIKSISVWVKGRNYDNGFAIRLKDR
                     EGIEREYYLGSLLFDNWRELTWVNPNYIENPKDRILQRLPLYPRSRPYIKFTSFVVYR
                     QMDSVGGDFVLYIRDVKMAYDKAIPDDIDKDDFQDENIWGILSKAREEEKERELLRLA
                     ERKTLLKQEESKLLIETNKRP"
     gene            1758..2966
                     /locus_tag="PLD82_02075"
     CDS             1758..2966
                     /locus_tag="PLD82_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG64206.1"
                     /translation="MGISESFGQKIRTGIVIVLALLLHSHSLSAGAVPEGVQKIRVET
                     DFEGGNGCLIKSDVRTNSVVLAPEKKPGDTLALNLYCVLTGFDPRRELQIHLKSPAPP
                     RGLFVREGRKVWRQIQSDRAGRYRGRFPTGRVELATMPPYLFSDLQRWLTAQPAREWF
                     RIVYPGKTGQGLALPVVLLTPKNAGKKTPLIVIIARQHAFEHAASWVVQGMLDFLVSN
                     TTDARAGRNKAVFAIVPMVDIDGVRLGSTGKHLGGTDMNRSWDSPVHREQGVILDWID
                     GLAERYPVHGVYDIHAMGYQMPSFRFGLSLFDNTRGNPDRAWRYRELSRRFRNISGYA
                     PARLPLPGWKNLPGMFATRMLNHWPNLREAIIVECTPWRRPDGVCWNAELIRRAGVDL
                     ARAVCRGIYG"
     gene            2959..3813
                     /locus_tag="PLD82_02080"
     CDS             2959..3813
                     /locus_tag="PLD82_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG64207.1"
                     /translation="MASNTIRTIVAVCCILPWVPQVFNAAEDAPHEGVAIMRHMATVY
                     GLPLREDGPGVRLVLPDGKTIPYQDDRILAGHPWAIPDIRGLFRQPYPGTNVRFGIRD
                     RERDPGRYRNYQLLGAVYGASQAEVRRLLTSVDFCGTRVLFSTRNGAAAALARVSDEI
                     RKRPLLLAWIRQLVGRDSQAIGTWCWRTIAGTGNLSPHSYGIAIDIYDRRSPRISYWR
                     FVSPDPQKASRWPELVSHERIWQPPQELVYCFEKHGFVWGGKWYHFDPMHFEYRPEFF
                     GLEAVREP"
     gene            3803..4681
                     /locus_tag="PLD82_02085"
     CDS             3803..4681
                     /locus_tag="PLD82_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOG64208.1"
                     /translation="MSPSIPGPRIVWALLAVCMGGFSLQADPGPLRACIRPGDSLYIQ
                     DIRSGEVLNLDDRKPVPAASLVKLFVMVEAFKAFGDGRLDKNGSVTVSPQDIVGGAGV
                     IRLGGAGCRYTWYRLVQLMIQESDNIAANLLIQKIGMLAINETARDMGFHQTRVVRMM
                     MDTVAMEQGLENMTSARDVGRLLKSIAEGQCLDEAVSREMMKFLSGQKRPGKLGSLLP
                     ASASIAHKTGELVLPGAVRVEGDAGIISGPGGRIVMVVISVGDCASVAHLAAMGKLAW
                     EHFGLADRGVSHGSQL"
     gene            4746..5561
                     /locus_tag="PLD82_02090"
     CDS             4746..5561
                     /locus_tag="PLD82_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOG64209.1"
                     /translation="MMRLRRLVTSVFAFLLLGTSGLVANDAREVVKEAIPGVVLVVRP
                     WHTSWPSRVRATGTGFVIHRDGYVLTAFHNIGSTETGRIDSILYAYFVDKKHPYDPPR
                     RQAWRLRVVRTNFMLDLALLKIDAMEKDGRLVAVPRGTKFPTLEIADSDDVEPTDDVH
                     ALGFPSLARTGDSIFTGITVTSGKVVGLDRENKWLKSGASISPGNSGGPAIDSKGRVI
                     GVNTMVRLEHRTQGRIALVRPVNLARHLAKDTPAWGAFEDGPELVSDDEREPD"
     gene            complement(5678..6412)
                     /locus_tag="PLD82_02095"
     CDS             complement(5678..6412)
                     /locus_tag="PLD82_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG64210.1"
                     /translation="MSANTQLVCEMLEAMVRLSDEVSTLGFGPHAAAVYNPLVYARAG
                     AEAYIRRYARHCRALLIGMNPGPFGMAQTGVPFGEIAAVRDWLGIAAEIGQPEVVHPK
                     RPITGFACTRSEVSGRRLWGFFREVYGNPDGFFDHFHVANYCPLVFMEATGRNLTPDK
                     LPVGERTPLYTACNRHLSRIATILEPEWIIGLGAFATKRSQEVLGTGYRYLTLPHPSP
                     ASPLANRGWNQLARQALAPLGFVPGC"
     gene            6606..>8102
                     /locus_tag="PLD82_02100"
     CDS             6606..>8102
                     /locus_tag="PLD82_02100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG64211.1"
                     /translation="MADGVVRRIFQAVFGKKIAGTRIFPITTKIIIIFTLFMIGSNIT
                     TNYINLVFSREEMSKLLKELLVKDLRELYSYLNNQHEIYRFSNDLKQVVGQIEEKSRR
                     DFSKASSLAIGIKTDGTIFFAATGSTNTDSRESKADSAAVAEMTRSLNKGKMDGFVRL
                     HHGGAEYYTVYRYHPKWDAFLVRGEEYGEFFAQSERIFVNVAIISLLITLACALIGIM
                     LLRFILRFLHRITTDIIAMTGQQQLGLVNLQGASNDDITFLGTAFNSLSSTINSMITI
                     FKKFVNQDVVAQAYREKVVRLEGSTRDLTCLFSDIKRFTFITETLGQDIITLLNLHYG
                     NAIREIMRHDGIIGSIIGDALLSVYGVLDESNDNKSLQSLRSAWAIQSEAALLRERMT
                     EIKRNVEKQQGRLSDAEERVYQAVLIEVGVGIDGGMVFYGNIGSNERMTNTVIGDNVN
                     SASRLEGLTRVYHVPVICSEYVKNDVEANVPGHGYIFVELDTVQVKGKT"
BASE COUNT         1767 a         2042 c         2483 g         1810 t
ORIGIN      
        1 gacgttgatg tttgccgatt tgcgatatgc cagactggtt cccgtaaccc gggaggttcc
       61 gagggagcac tggcatcgtg atgcactcaa aactcttgtg caggccctgg ttgatggtcc
      121 ggtcaataat gatactctgc ccgttgttcc tgccgagacc cggattcttg gttgctggca
      181 ggacgggaag accgcctgga tcgattttga cagaaatatc tttcttggct tggcggatgc
      241 ggctgatgcc gaagtcctct gcgcctacgg aatcgtgaat acgatcataa agaacctccc
      301 ggatatcgaa cgggtgcaga ttctcgtcga gggagctcca cgacagactc tgcggggatt
      361 gaccagggtt gttcaacccc ttgaaccccg tacggatctt gagtaggagt gcgcttccca
      421 aaagggagtc aagttttccc cgatggaagc cgatactcca tctgagacat gaccgcgaat
      481 ttttcgtatt gacaagattt gacggtatgc ctaaaataca aaaaacttcg ggcagtcaac
      541 catggaaagg agtccggtga tgagaaaggc cacgcgaatt gcaggaatag tgctgctcgc
      601 tgtatgcatg ttcctgatga cactggcata tgcacaaaca gtagacgcaa caggcagcca
      661 gagtggacgt cttgtggcca acagggcgtt tgtcattgat tttggacagt atgagtcaag
      721 aatgaaggct cagatcgagc gcaacaaaaa gctgatcgag agtcgtacga atgcttcagc
      781 ggcacggggt ttgccaactg aaaaatttac catggaagct tcagattggg ccctcgacaa
      841 atgggaagtc gagctcaatt cttcggcatc cagcatcatg aacaacgtgc tctcgtacgt
      901 tgtcaaggtc ggaagcccct ggtggctgaa taatgccaaa agaatctatt ccggcatggc
      961 aggcaagctt gaaggataca atgagacacc ggccagcaac gtccttggtg tccgtatcca
     1021 cttcccgacg catcggcaaa actgctgggc caagatccat cctccgttcg agatcaacgc
     1081 ctatgacgac aagggcgggc tcgccaacac caacaacggt atcgtggata acgttggaca
     1141 gatcaagagc atctcggttt gggtgaaggg gcggaattat gataacggct tcgcgattcg
     1201 tctcaaggac cgggaaggga tcgagcgcga gtattatctg ggatcactcc tctttgacaa
     1261 ctggcgcgag cttacctggg tcaaccccaa ctacatcgag aacccgaagg accggattct
     1321 gcagcgcctg ccgctctatc cgcgcagccg gccctacatc aagttcacgt cctttgtggt
     1381 gtaccgccag atggattctg tgggaggcga cttcgttctc tatatccgtg acgtcaagat
     1441 ggcctacgac aaagcaattc cggatgacat tgacaaagac gatttccagg acgagaatat
     1501 ttggggcatc ctctccaagg cacgtgagga agagaaggaa cgtgagctcc tgaggctcgc
     1561 cgagcgcaag accttgctca agcaggaaga atcaaagctc ttgattgaaa ccaacaagag
     1621 accgtaatcg tggcggatga ttgactcgaa tgtacaatag aagaggaagc caccttgagg
     1681 ggtggcttcc tctttcttga cttcagacag cggacatgga tcgggagtag atcggcacta
     1741 tgcaatctgt gcggagtatg ggcatcagtg agtcctttgg gcagaagatt cggaccggaa
     1801 tcgttatcgt gttggcgctc ctcttgcatt cacattcgct ttcagccggg gctgtcccgg
     1861 agggtgttca gaaaatacgg gtcgaaacgg actttgaggg tgggaatggg tgtctcatca
     1921 aaagcgacgt ccggaccaat tccgttgtcc tcgcgcctga aaaaaaaccg ggtgataccc
     1981 tggccttgaa cctctattgt gtcctgacgg gttttgatcc ccggcgggaa ttgcagatcc
     2041 atctgaaaag tccggcacct ccgaggggac tctttgtccg ggaggggcgg aaggtatgga
     2101 gacagattca atctgatcgg gctggacggt atcgcggccg gtttccaact ggtcgggtcg
     2161 aactggcgac catgccaccc tatctgtttt cggaccttca acgctggctg acggctcagc
     2221 cggcgaggga atggttccgg atcgtgtatc cggggaaaac cggacagggc cttgcccttc
     2281 cagtggttct tttgacaccg aaaaatgcgg gaaaaaaaac tccgctgatc gtcatcattg
     2341 ccaggcaaca tgcttttgag catgcggcca gctgggttgt gcaggggatg ctcgattttc
     2401 tcgtctcgaa taccacggat gcccgggctg gaaggaacaa ggctgttttt gcgattgtcc
     2461 cgatggtcga tattgatggt gtccggcttg gctcgaccgg aaagcatctt ggcgggacag
     2521 acatgaaccg gagttgggac agtcctgtcc accgggagca aggggtgatt ctggactgga
     2581 ttgatggttt ggccgaacgc tatccggtcc acggggtcta tgatatccat gccatggggt
     2641 accagatgcc gtcgttccgg ttcggactga gcctcttcga caacacaagg ggaaatccgg
     2701 acagggcgtg gcggtatcgg gaattgtccc ggagattccg gaatatttcg ggttatgccc
     2761 ctgccagact gccgctgccg ggctggaaga atttgccggg catgtttgcg acgaggatgc
     2821 tcaatcactg gccgaacctt cgggaagcca ttattgttga gtgtacacca tggcgtcgac
     2881 cggatggggt ttgctggaat gcggagttga ttaggcgggc cggagtagac ctggcaaggg
     2941 ctgtctgcag ggggatatat ggctagcaac acgattcgga cgatagtggc ggtctgctgc
     3001 atcctgccat gggttcctca agtcttcaac gcggctgagg atgccccgca tgaaggtgtc
     3061 gccatcatgc ggcatatggc cacggtctat ggcttgccgc ttcgggagga tgggcccggg
     3121 gtcaggctgg tgcttcccga cgggaagacg attccgtatc aggatgacag gattctggcc
     3181 gggcatccct gggccatacc ggatatccgg gggctcttcc gtcaaccata ccccgggaca
     3241 aatgtccgct tcgggatccg ggatcgcgag cgtgatccgg gccgatacag gaactatcag
     3301 cttttgggag cggtctacgg ggccagtcag gccgaggtcc ggcgtttgct gacgagtgtg
     3361 gatttctgtg gaacccgggt cctgttttca acccggaacg gggctgcggc tgctctggca
     3421 agagtctcgg acgagatacg aaagagaccg cttttgctgg cgtggatccg gcaacttgtg
     3481 ggtcgggaca gtcaggccat cggaacctgg tgctggcgga cgatcgccgg gacaggaaat
     3541 ctctctccac actcctacgg gattgcgatc gacatctatg atcgcagatc accccggatt
     3601 tcgtattggc gcttcgtctc accggatccg cagaaggcat cccgttggcc cgaactggtt
     3661 tcgcatgagc ggatctggca acctccgcag gaactggttt actgcttcga gaagcatggg
     3721 tttgtctggg gtggaaagtg gtatcacttt gatccgatgc attttgagta ccggccggaa
     3781 tttttcgggc tggaggctgt tcgtgagccc tagtatcccg ggtccccgca tcgtctgggc
     3841 attgcttgcc gtttgcatgg gagggttttc actccaggcc gatccgggcc cgttgcgcgc
     3901 ctgcatccgt cccggggaca gtctctatat ccaggatatc cggagtggcg aggtgctcaa
     3961 cctggatgac cgcaagcctg tgccggctgc cagtctggtc aagctctttg ttatggtgga
     4021 ggcattcaag gctttcgggg acggtcggct cgacaaaaac ggaagcgtga ccgtttcccc
     4081 gcaggatatt gtcggagggg caggggtgat tcgtctgggc ggagcggggt gtcggtacac
     4141 ctggtatcgt ctggtgcaat taatgatcca ggaaagcgac aatattgcgg caaatctgct
     4201 gatacagaaa atcggaatgt tggctatcaa cgagacagca cgggacatgg gcttccatca
     4261 gaccagggtt gtccgcatga tgatggatac cgtcgccatg gagcagggtc tggaaaatat
     4321 gaccagtgcc agggatgtcg gccgtctgct gaaaagcatc gcggaaggac aatgcctgga
     4381 cgaggccgta tcccgggaga tgatgaagtt cctctctggc caaaaacgcc cgggcaaact
     4441 cggaagtttg ctgccggcaa gcgccagtat cgcgcacaag accggggagc ttgttctgcc
     4501 gggcgcggtc cgggttgagg gtgatgcggg cattatcagc gggcccgggg ggcggattgt
     4561 gatggtggtc atctccgtgg gcgattgcgc atcagtggcg catctggcag ccatgggaaa
     4621 gctggcatgg gagcatttcg gtctcgctga ccggggtgtg agccacggga gccagctgta
     4681 acagagcgtt atcctgggtg tatgatgtgg gcatgactgt gatacttata ttccaggaag
     4741 gtgcgatgat gagattgaga cgactggtaa cttccgtatt tgctttcctg ttgttgggga
     4801 catcgggttt ggttgccaat gatgcccggg aggtcgtcaa ggaagcgatc cccggagtag
     4861 tccttgtggt tcgcccctgg catacttcat ggccttccag agtccgggct acagggaccg
     4921 ggttcgtgat tcaccgggac ggctatgtgc tgacagcctt tcacaatatc ggttcgacgg
     4981 agacgggccg gattgactcc attctgtatg cctactttgt cgacaagaaa catccctatg
     5041 atccgccccg caggcaggct tggcgtttgc gggtggtcag gacaaatttc atgctggatc
     5101 ttgcactgct caagattgat gccatggaga aggatggccg attggtcgcg gtcccccggg
     5161 ggacgaagtt cccgaccctc gagatcgccg attcggatga tgtggaaccc accgatgatg
     5221 tgcatgccct cggctttcct tctctggcga ggacgggaga ttccattttt accgggataa
     5281 ctgtcacctc cggcaaggta gtcgggctgg atcgggaaaa caaatggctt aaatcggggg
     5341 cgagcatctc tccggggaat tccggtggtc ccgcgattga cagcaagggg cgcgtcatag
     5401 gggtgaacac gatggtccgc ctggagcaca gaacccaggg ccggatagcc ctcgtgcgac
     5461 cggtcaatct ggcgcgacac ctcgcgaagg acacaccggc ctggggagcg ttcgaggatg
     5521 gtccggagct ggtctccgat gacgagcggg aaccggattg aagggtgatt gtggtcttgt
     5581 ctgctctgtc tgtgaaagaa ccgttgcgtg acaggccgga ttgaagcagg cctgaggatg
     5641 ttcctcaaaa tatcattgca agccggtgag gggcccttca acatccgggg acgaagccga
     5701 gtggtgccag ggcctgccgt gcgagctggt tccacccacg atttgcaagg ggactggcgg
     5761 gactggggtg cggcagggtc agatagcggt acccggttcc gagaacttcc tggctgcgtt
     5821 ttgttgcaaa ggcaccgagc ccgatgatcc actcaggctc gagaattgtt gcgattcgtg
     5881 agaggtggcg gttgcaggcg gtgtagagcg gggtccgttc gccaacgggg agtttgtcgg
     5941 gggtgagatt gcgcccggtg gcttccataa agacaagggg acagtaattt gcgacgtgga
     6001 agtgatcaaa gaatccgtcc gggttgccgt agacctcccg gaaaaacccc cagagccttc
     6061 gtccgctgac ctcgcttcgc gtgcaggcaa agcccgtgat gggccgtttt ggatggacaa
     6121 cttcaggttg tccgatctct gcagctattc cgagccagtc gcggactgct gcgatctctc
     6181 cgaagggtac gccggtctgc gccatgccaa agggtccggg gttcattccg atcaggagcg
     6241 cccgacagtg tcgtgcatag cgtcgtatgt atgcttccgc tccggcccgt gcgtacacca
     6301 ggggattgta gactgcggcc gcgtgtggtc cgaatccgag ggtggaaact tcatcggaaa
     6361 gacggaccat ggcttccagc atctcacaga ccagttgtgt gttggcggac accggatcct
     6421 cctgcaacag ctcgttcgaa ctacggtact cttgaattct acgcattttt ttactgcaat
     6481 tgacggcgga acttgataat atcaagggat gatgccagga tgacagcaat gatcacccgg
     6541 cgacagggtg tcgcttctgg ccgggaccaa gacgtatcag aatgggttgt gcaagggggc
     6601 tggcaatggc cgatggcgtt gttcgacgca tttttcaggc tgtcttcgga aaaaaaattg
     6661 ccgggacacg catttttccc atcacgacca agatcatcat aattttcacg ctttttatga
     6721 tcggctcaaa cataacaacg aattacatca accttgtttt cagccgcgag gagatgtcaa
     6781 agctcctcaa ggagcttctg gtcaaggacc tcagggaact ctattcctac ctcaacaacc
     6841 agcatgagat ctatcgcttc agcaatgacc tcaaacaggt tgtcgggcag atcgaggaaa
     6901 aatcccggcg tgatttcagc aaggccagtt cgctggcgat cggtatcaag acggacggca
     6961 cgatcttttt tgcggcgacc ggttcgacaa ataccgacag ccgcgagagc aaggcagatt
     7021 cggcggcggt cgccgaaatg acacgcagcc tcaataaagg caagatggac gggtttgtcc
     7081 ggttgcacca tgggggggcg gagtattaca cagtctaccg ctatcatccc aagtgggatg
     7141 cctttctggt tcggggcgag gagtatgggg agttctttgc acagtcggag cggatctttg
     7201 tcaatgtggc catcatcagt ctcctgatta ctctggcctg tgcattgatc gggatcatgc
     7261 ttctgcggtt catcctccgt ttcctgcaca ggatcacgac ggacatcatt gccatgacag
     7321 gccaacaaca gctcggactg gtcaatctcc agggtgcgag caatgatgat atcacgtttt
     7381 tggggacggc cttcaactcc ctctcgagta ccatcaacag catgatcacg attttcaaaa
     7441 aatttgtgaa tcaggatgtg gtggcccagg cctaccggga aaaagtcgtc aggcttgaag
     7501 gctctacacg tgatttgacc tgcctttttt cggatatcaa gcgcttcaca ttcatcaccg
     7561 aaacactcgg gcaggacatc ataacgctcc tcaacctgca ttacgggaac gccatccgcg
     7621 agatcatgcg acacgacggg atcatcgggt caatcatcgg ggatgcgctc ctctcggttt
     7681 acggcgttct ggatgagagc aatgacaaca aatccctcca gtcactgcgt tcggcttggg
     7741 cgatccagag cgaggcggca ctcctccgcg agcggatgac cgagatcaag cgcaatgtcg
     7801 aaaagcagca ggggcgtctg agcgatgccg aggaacgtgt ctatcaggct gtcctgatcg
     7861 aggttggcgt cggtatcgat ggcggcatgg tgttctacgg aaatatcgga tccaatgagc
     7921 ggatgacgaa cacggttatc ggggataatg tcaactccgc atcacggctt gaggggttga
     7981 cgcgggtcta ccacgtgccg gtcatctgtt cggaatatgt caaaaacgat gttgaagcga
     8041 atgtacctgg acatggctac atttttgtcg agctggatac ggtccaagtc aaaggaaaga
     8101 ct
//
LOCUS       DAOSHE010000040         8102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7618_length_8102_cov_3.811980, whole genome shotgun sequence.
ACCESSION   DAOSHE010000040 DAOSHE010000000
VERSION     DAOSHE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7618_length_8102_cov_3.811980"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLD82_02105"
     CDS             <1..1047
                     /locus_tag="PLD82_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG64212.1"
                     /translation="VFLSSRRMAMLETVVFPMASLMVNVSQAVVLGYGGLEVIAGRLT
                     AGDILLFNVYMAGLAFPLAFMGGILALLERARSAEGRISEILDLPVEDMVSGLVPGPE
                     KSAALALEVQNLSFSFPSGFALADCSFSVSRGGRIGICGPVGSGKSLLLDLVARFEEP
                     PPATIFVLGEDVLGQKVGELRTRVGYALQTARLFSDTVRNNLVFGLEREVTDEEIHGV
                     LHKAAILDEILSFPDGLETVTGEKGMRLSGGQRQRLALARLLLRNPPLLLLDDVFSAV
                     DQSTEARLVEEMFSGTASILVVSHRHAVLERCDEVLLMQDGRILDRGSYEGLAQRHRR
                     LLQTMAREQIHETG"
     gene            1034..2806
                     /locus_tag="PLD82_02110"
     CDS             1034..2806
                     /locus_tag="PLD82_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG64213.1"
                     /translation="MKPAKPGQQGGLRLLWRLGPWILKHKRLFLTVLALAPLSAGLQM
                     ALPNLIRLVIDKGMLSRDTEALTGFTALFVGAFLADFCVRSLQRYWTAVLVQKTVRDL
                     RQDLTNRLVSLPAAFHDGQISGVLVTRATSDFDALNESLGHGVFGALADMVTIGGSLA
                     ALFLIAGPLALVALCILPLVGLVTGLCARALQRAMLAARARLAELNGFTQECITAMPA
                     IRSLNAGKDAGHRYDRASLAYRDAQMKSVVLDAVLYSVVEGLAAIALGVLLWLVSGDL
                     VTGVSAGVLIASVSSLQQIFEPLKEFTNRIALLQGVFTALERIFGLMDVRIKSGGSIV
                     PTCRKGELEIRELRFSYDPVESGREARMILEEISVSVTPGQSLALVGATGCGKTTLMR
                     LLAGEYDTYHGSVRIDGQEVAQLDPALHRASLGIVPQDPEIFAGSIEFNISLGRRGVT
                     RDAVRRAAESVGAARFIERLPEGYETILEENGANLSQGQRQLVVFARAIVTEPAMLLL
                     DEATSSLDPESEHLVQAAMDALFVGRTVVVIAHRLSTVERCDRIVVLENGRVAESGTH
                     QQLLHSRGVYCAMVRSFVDRKKRT"
     gene            2931..4169
                     /locus_tag="PLD82_02115"
     CDS             2931..4169
                     /locus_tag="PLD82_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOG64214.1"
                     /translation="MKDRTIVLFEDVYTYHLYPFHLVRPVCDMASGMFTLYERMARLY
                     PQSRIEILCRPEIRDLVRESGRFLFDESAFKHAERYVFINARTAFAARHRFDIDTCCI
                     EQDTLVYLHVGPGRISEDWSQRDFLDGECLEKARMLGLDERRPEHKLFQYPWDIVNHV
                     GKAIEHDALIAAPVWTQPGEGMLGDRIALGPDVRVDPGCILDSRQGPVILEAGVSVSQ
                     RSIITGPAWIGAGASIDAARIHSGVSIGQGCRIGGEVECSIVYPWTNKHHDGFLGHAC
                     VGSWVNIGAMTTNSDLRNDYGEITVRMQGREFRTGQIKLGVMIGDFSKLGIGLLLNTG
                     TVLGVGANLWFDGALIKGEVHSFVWGGAAPWSEYRINKFLDTMQTVMQRRGQKPGDAL
                     RKRLLDLHAETGDQRRNGSG"
     gene            4209..4637
                     /locus_tag="PLD82_02120"
     CDS             4209..4637
                     /locus_tag="PLD82_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64215.1"
                     /translation="MRLVGLVLIVALLAGLPLVLSIWKEMTVVPWYLVGVLLFCLGNV
                     VFSYSFREMGASWKLFLKNEKTEPGRHKTLALMHEALSRQALAGGFLVLLAGWIAVFC
                     LAQPPLVQDFGRALSGPFFALILGWGLHRPLANWHRRMAE"
     gene            complement(4747..7194)
                     /locus_tag="PLD82_02125"
     CDS             complement(4747..7194)
                     /locus_tag="PLD82_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOG64216.1"
                     /translation="MGRFSLSLRTLERALFVILLSMAIVFGLSIAYIISTLSSMEAIE
                     KLEYFRNKIPTKVYDVKGRLFAEFFYQKREVIHFDKIPKNLVHALVAIEDNSFFSHGG
                     IDVGGMFRALLVDVFTGSAKQGGSTITQQLAKRLFTESEKTIARKIRELWYALQIEKK
                     YSKQEILELYCNEIYFGHGAYGIEAASQIYFQKSARDINLGECAILAALPSAPNAYSP
                     IAFPEMARQRQRIVLLKMAEMGYITKQEADKAWEGFWEEYSQKNLAPTLSAWNVKIDK
                     APYFTEYIRQQLVDIYGVDRVNEDGLRVYTTLDLDMQQAGERVLQEKLFQMRTNYQRS
                     YQVYVSDVRKKHLDPIDLISSIFDIGSIRISGVKTSTYVNDSLESTWKSPLLMLSFIY
                     GLQGMNSVIDGAYNWDPKSSRGVSPEGALVAIDPRNGYVKTLIGGSEFSPENRFNRAV
                     QAYRQPGSAFKPFVYLAAIESKDLTAGSALEDAPVAYEQANGVLWFPRNYDRHYSGRL
                     VLRDALRWSVNIITVKILDIVGYERITKIASTLMHIPPADIEKRFVRAHSLALGGSEV
                     TPYELCNAFAVLANEGQDVQPISLLKVYDRDGKLIDDFLRKRDYDKDERPKPNQQLVE
                     KEPVYILTTMLEDVTADGTAAKAKEDAGLRRPIAGKTGTASNWKDVWFACYTPQIAST
                     VWIGFDDPGISLGRGSSASDVAAPIVLKFMAYALRNEPERWYKPPAGVHKGSVCRVSG
                     KAPTKACRKVYGEWFLEGTGPDGKCEECERIGSAITNYRGTARVDDGGTKRDPTRDDN
                     GLSITGPRISTDNLFDD"
     gene            7469..7813
                     /locus_tag="PLD82_02130"
     CDS             7469..7813
                     /locus_tag="PLD82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG64217.1"
                     /translation="MIEFRSDMQTLDFENCSKLLKAMAHPVRLRIIFGVARKECNVKS
                     MWQCLNLPQDVVSQHLAILRKKNILKTTKKGNSVFYSINPELDLSCVLDFMANCKGLQ
                     NPKKKSGVQRDQ"
     gene            7803..8009
                     /locus_tag="PLD82_02135"
     CDS             7803..8009
                     /locus_tag="PLD82_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOG64218.1"
                     /translation="MINKRAILLSLSGIVVGGLGGWLYYHFIGCEAGTCPITSRPVPS
                     ILYGALVGFLLSGLGSGWKTRKKP"
BASE COUNT         1621 a         2153 c         2412 g         1916 t
ORIGIN      
        1 gtctttctca gcagccgccg gatggccatg ctggagacgg ttgtcttccc catggccagt
       61 ctgatggtca atgtctcgca ggccgtggtc ctcgggtatg gaggcctgga ggtcattgcc
      121 gggagattga ctgcgggtga tatcctgctc ttcaacgtct atatggcggg actcgcattt
      181 ccgcttgcct tcatgggcgg gatcttggcc ttgctcgaaa gggccagaag tgccgaggga
      241 aggatttcgg agattctgga tcttcccgtt gaggacatgg tatcgggctt ggtcccggga
      301 ccggaaaaat cggctgcgct ggcgcttgag gtgcaaaacc tgtccttttc ctttccatcc
      361 gggtttgccc tggcagactg cagtttctcg gtttcccggg gcgggcggat cgggatctgt
      421 ggccctgtcg gttcaggaaa gagtctcttg ctggatcttg ttgcacgctt tgaggaaccg
      481 ccgcctgcga cgatcttcgt tttgggagag gacgttttgg gacagaaggt cggcgaactc
      541 cggacccggg tcgggtacgc cctgcagacg gcaaggctgt tttcggatac cgtccggaat
      601 aatctggttt tcggtctcga gcgagaggtc acggacgaag agattcacgg agtcctgcac
      661 aaggccgcca tcctggacga gattctttcc ttcccggatg gtctggagac tgtcaccgga
      721 gaaaagggaa tgcggctctc gggagggcag agacaacggc tggctttggc cagattgctg
      781 ctccgcaatc caccgctcct tctgctcgac gacgtctttt ccgccgttga tcagtcgacc
      841 gaggccaggc ttgtcgagga gatgttctcc gggacagcat ccattctggt cgtgtcacac
      901 aggcatgcgg tcctcgaacg gtgtgacgag gtcctcctga tgcaggatgg ccggatcctg
      961 gatcgcggga gctacgaagg actggctcag cgacatcgaa gattgttgca gaccatggcc
     1021 agggagcaga tacatgaaac cggctaaacc ggggcagcag ggaggcttgc gtctcctctg
     1081 gcggcttggt ccatggatcc tgaagcataa acggctgttc ctgacggttc tggccctcgc
     1141 tcctctgtct gccggtttgc agatggcgct tccgaatctg atccggttag tcatcgacaa
     1201 aggtatgctc tcccgggaca cggaagcgct gacgggtttc accgccctct ttgtcggagc
     1261 atttctggcc gatttttgtg tgcgctccct gcaacgatac tggacagccg ttcttgtcca
     1321 gaagacggtc cgcgatcttc gacaggacct gacaaaccgg ctggtttccc tcccggccgc
     1381 gttccatgat gggcagatat ccggtgttct cgtgacccgg gccacgagcg acttcgatgc
     1441 cctcaatgaa tctctcgggc atggcgtgtt tggcgccctg gcggacatgg tgacgatcgg
     1501 gggatccctg gcagcactct ttctgattgc tggaccgctt gcccttgtgg ccctctgtat
     1561 cctgcccctt gtcggacttg tgaccgggct ctgcgccagg gctctccagc gggccatgct
     1621 cgcggcccgc gcccgtctgg ccgagctgaa cgggtttacc caggaatgca tcacggccat
     1681 gccggccatc aggtccttga atgccgggaa ggatgccgga catcgttatg accgggcgag
     1741 tctggcctac cgggatgccc agatgaagag tgttgtgttg gacgctgtcc tctattcggt
     1801 cgtcgaggga ttggcagcga tcgccctcgg tgtcctgctc tggttggtga gcggggatct
     1861 cgtgacggga gtcagtgccg gtgtgctgat tgcttccgtg tccagtctcc agcagatctt
     1921 tgaaccactc aaggaattta caaacaggat tgctcttctc cagggcgtgt ttaccgcact
     1981 ggagcggatt ttcggtctga tggacgtcag gatcaagagc ggagggagta tcgttccgac
     2041 ctgccgaaaa ggggaactcg agattcgcga gctccgcttt tcctatgatc ctgttgaatc
     2101 cgggagagag gcgcggatga ttctggagga gatctcggtc agtgtcaccc cgggtcaatc
     2161 gctggcgctg gtcggtgcaa caggctgtgg aaaaacgaca ttgatgcgat tgcttgccgg
     2221 agaatatgat acatatcacg ggagtgttcg gatcgatggc caagaggttg cgcagctcga
     2281 tcctgccctg caccgggcaa gtctgggaat tgttccccag gacccggaga tttttgccgg
     2341 aagcattgaa ttcaacatat cgcttggccg aagaggcgtc acacgggatg ccgtcaggag
     2401 ggctgcggaa tcagtgggag ccgcgcgctt cattgaacgg cttccggagg gatatgaaac
     2461 cattcttgag gaaaacggag cgaatctttc ccaggggcaa cgccaactcg ttgtgttcgc
     2521 ccgggccatc gtgacagaac cggccatgct cctccttgat gaggccacgt cttcccttga
     2581 tcccgaaagc gagcacctcg tgcaggcggc catggatgcg ctttttgtcg gcaggacagt
     2641 cgtggtgatt gcccatcggc tttccacggt ggagcggtgc gacaggatag tcgtcctgga
     2701 aaacggccgg gtggccgagt cagggacaca ccagcaactt ctgcattccc ggggtgtgta
     2761 ttgtgcgatg gtcagatctt ttgtagacag gaaaaaacgg acgtaatttt tgcggttcgg
     2821 gaattctgtg tcacccgctt ccaccctcgg gcgtcctgtt tttccttgca ggagtccggt
     2881 ctgacaccct atactgcaac ccggatggaa tcatttggag gccgacagtc atgaaagacc
     2941 gaaccatcgt actctttgag gatgtctata cataccatct ctatccgttc catctggtcc
     3001 ggcctgtttg tgacatggcg agcggtatgt tcacgttgta cgagcggatg gccaggctct
     3061 atccccagag ccggatcgag atcctctgca ggcctgaaat ccgggacctt gtccgggaga
     3121 gcggtcgctt cctctttgac gaaagcgcgt tcaaacatgc tgagcgctat gttttcatca
     3181 acgccagaac ggcgtttgcg gctcgtcacc gttttgacat cgatacctgc tgcattgaac
     3241 aggatacact tgtctatctc catgtcggac ccggccggat atcggaggac tggagtcagc
     3301 gggattttct cgatggtgaa tgccttgaaa aggcccggat gctgggactg gatgaacggc
     3361 gccccgaaca caaactcttc cagtacccgt gggacatcgt caatcatgtc ggtaaagcga
     3421 tcgagcatga cgcactgatc gccgccccgg tatggactca gcccggagag gggatgcttg
     3481 gcgaccggat tgcactgggg ccggacgtcc gggtcgatcc gggttgcatc ctcgattccc
     3541 gccaggggcc ggtcatcctc gaagccggtg tctctgtcag tcaacgaagc atcatcaccg
     3601 gaccggcatg gatcggtgcc ggagcgtcga ttgatgcggc acggatccac agtggcgtca
     3661 gtatcggaca ggggtgccgg atcggtggcg aggtggagtg ctcgattgtc tacccgtgga
     3721 ccaacaagca tcatgacggt tttttgggtc atgcctgtgt gggttcatgg gtcaatatcg
     3781 gagccatgac aaccaattcc gacttgcgga atgattacgg ggagatcact gtcaggatgc
     3841 agggacgtga attccggaca ggccagatca aactcggtgt catgattgga gacttcagca
     3901 aactgggaat cgggctgctc ctgaataccg gtaccgtgct cggtgtcgga gccaacctct
     3961 ggtttgacgg tgccctcatc aaaggggaag tccacagttt tgtctggggt ggtgctgcgc
     4021 cctggtccga gtaccggatc aacaaatttc tcgataccat gcagacggtc atgcagaggc
     4081 gggggcaaaa gcccggagat gccctgcgga aacggcttct ggatcttcat gccgagacag
     4141 gtgatcaacg gagaaatggt tccggctaaa atcggccggc gacattgcgt attgaaaagg
     4201 agaaaagtat gcgtttagtt ggactcgtcc tgattgtggc cctgctggca ggcctgcccc
     4261 ttgttttgtc gatctggaaa gaaatgacgg tcgtcccctg gtatctggtc ggagtgcttc
     4321 tcttttgtct cgggaatgtt gtgttttcgt attctttccg cgaaatgggt gcttcctgga
     4381 agctcttcct caagaacgaa aagaccgagc ccggtcggca caagactctc gcgctcatgc
     4441 acgaggcgct ctcgagacag gctctggcgg gcgggtttct cgtgttgctt gctggctgga
     4501 tcgccgtgtt ctgtctggcg caaccacccc ttgtccaaga tttcggtcgg gccttgtccg
     4561 gtccgttttt tgcgctcatc ctgggatggg ggctgcaccg tcccctcgca aactggcacc
     4621 gtcggatggc ggagtaagac gcacccgccc tcccgggcgt catcttcacg gtcgtgatcg
     4681 gaaaagagaa ggggccgtct ttcgggacag ccccttcttg cgttctgtca gtgaccaggc
     4741 ggggtctcaa tcatcaaaaa gattgtcggt cgagattctc ggcccggtga tcgaaagtcc
     4801 gttgtcatcc cgggttggat cacgctttgt accgccgtca tcgactcggg cagtgcccct
     4861 gtagtttgta atggcacttc cgatccgttc acactcttcg cattttccgt cgggaccggt
     4921 cccctcaaga aaccactccc cgtagacttt cctgcacgcc ttggtcggtg cttttcctga
     4981 aacgcggcag accgatccct tgtggacacc tgccggtggt ttataccagc gctctggttc
     5041 gttgcgcagg gcgtatgcca tgaatttcag gacgatcggg gcggccacat ccgaagcgga
     5101 tgaacccctc ccgagggaga tgccgggatc gtcaaatccg atccagactg tggacgcgat
     5161 ctggggtgta tagcatgcga accagacgtc tttccagttg ctggctgtac ctgtttttcc
     5221 ggctatcggg cgtctcaaac cggcatcttc cttggccttg gcggccgtac catcggctgt
     5281 gacatcttca agcatggttg tcaggatgta gaccggctcc ttttcgacga gttgctgatt
     5341 gggcttcggt cgctcatcct tgtcgtagtc ccgcttgcgc aagaaatcgt caatcagttt
     5401 tccgtcgcga tcgtagacct tgaggaggga aatcggctgg acgtcctgac cttcgttcgc
     5461 caacacggca aaggcattgc agagttcgta gggtgtcact tcagaaccgc ccagcgcaag
     5521 ggagtgggcg cggacgaagc gcttctcgat gtcagcgggc gggatatgca tcaaggtcga
     5581 ggcaattttt gtaattcgct cgtatcccac gatatccagg attttgacgg tgatgatatt
     5641 gacggaccag cgcaacgcat cgcgcaggac aagtcttcct gagtagtgac ggtcgtaatt
     5701 tctcgggaac cagaggacac cgttggcctg ctcatacgcc actggtgcgt cttccagagc
     5761 ggagccggcc gttaaatcct ttgattcgat agccgccaga tagacaaaag gcttgaaggc
     5821 cgaaccaggc tggcggtacg cctgcacagc acggttgaag cggttttcgg gagaaaactc
     5881 gcttccgccg atcagggttt tcacatatcc gttccggggg tcaatcgcga ccagagcgcc
     5941 ttcgggcgag acaccccgtg aactcttggg atcccagtta tacgcgccat caatgacgct
     6001 gttcatcccc tgaaggccat agatgaacga gagcatcaag agcggggatt tccaggtcga
     6061 ttcgagggag tcattcacgt aggtggacgt tttcaccccg ctaatcctga tggatccgat
     6121 gtcaaagatg gaactgatca gatcgatcgg atcgagatgt tttttgcgga cgtcagagac
     6181 gtagacctgg tagcttcgct ggtaatttgt ccgcatctgg aagagctttt cctggagaac
     6241 acgctcaccg gcctgctgca tgtccagatc aagggtcgtg tagacccgca gtccgtcctc
     6301 attgaccctg tcgactccat agatgtccac aagctgctgg cggatgtatt cggtaaagta
     6361 gggggccttg tcaatcttga cgttccaggc ggagagtgtc ggtgccaggt tcttctggct
     6421 gtattcctcc caaaaccctt cccacgcctt gtcggcttcc tgtttcgtga tataacccat
     6481 ttcggccatc ttgagcagaa cgatccgctg gcgttggcga gccatctcgg ggaaggcaat
     6541 cggggaatag gcatttgggg cggaggggag ggcagccagg atggcgcact ccccgagatt
     6601 gatgtcccgg gctgatttct ggaaatagat ctggctggct gcctcaattc cgtaggcccc
     6661 gtggccaaag tagatctcgt tgcaatagag ttcgaggatt tcctgcttgg agtatttctt
     6721 ttcgatctgg agtgcgtacc aaagttcacg gattttgcga gcgatggtct tttcgctctc
     6781 cgtaaagagg cgcttggcca gctgttgggt gatggtactc ccgccctgtt tggcacttcc
     6841 ggtaaagaca tcgacaagaa gggcccggaa cattccgccg acatcgatcc cgccgtggga
     6901 aaagaagctg ttgtcttcaa tggcaaccag cgcatggacc agatttttcg ggatcttgtc
     6961 gaaatggatc acttcgcgtt tttggtagaa aaactccgca aagagacggc ccttgacgtc
     7021 atagaccttg gtcggaatct tgttcctgaa gtattcgagt ttttcgatgg cttccatgct
     7081 cgagagggtg ctgatgatgt aggcgatcga gaggccgaaa acaatggcca tcgaaaggag
     7141 gatgacaaac agcgcacgct cgagagtgcg cagcgagaga ctgaaacgac ccatgacttc
     7201 tccagacctg tagggatcaa ttctgatgta aatctaccat aaaacagtgc aaaaaaaaag
     7261 ctgcctctcc gcataacggg agagacagcc ctgcctgaaa gcgttacttc agggaggaga
     7321 cgtatcggtg gatgggaggg gaaccgatac gctccacctt aaatgtatcg aatttttcga
     7381 gaatttcatt gaggaaaaaa tgatggggct tgacgtttca actttatggc gacatattca
     7441 tatatatgat aatcgggggc atcatctcat gattgaattt cgtagcgata tgcagacgct
     7501 ggattttgaa aactgttcga aactgctcaa ggccatggcc catcctgtcc gtctgcgcat
     7561 catcttcggg gtcgcacgca aggaatgcaa tgtcaaatcg atgtggcagt gtctcaattt
     7621 accgcaggat gtggtctcac agcacttggc catcttgcgc aaaaaaaaca tcctcaagac
     7681 cacaaaaaag ggtaattccg tgttttacag catcaatccg gaactggatc tctcctgcgt
     7741 gctggatttt atggccaatt gcaaagggct gcagaacccg aaaaagaagt ccggagtcca
     7801 gcgtgatcaa taaacgggcc atcctgctgt ccctgtccgg catcgttgtg ggagggctcg
     7861 gcgggtggct gtattatcac ttcatcgggt gcgaggctgg cacctgcccg atcacatcca
     7921 ggccggttcc ttcaatcctc tatggcgcgc tggtcggttt tctcctttcg gggctgggaa
     7981 gcggctggaa gacccggaaa aaaccgtgag tccggagcct gaaacctccg tcgagcatgc
     8041 ggttcttgta tccacaggtc ctgtgcccta tctttgcacg gtaaccctcc tcatccaagg
     8101 at
//
LOCUS       DAOSHE010000041         8016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7728_length_8016_cov_4.429469, whole genome shotgun sequence.
ACCESSION   DAOSHE010000041 DAOSHE010000000
VERSION     DAOSHE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7728_length_8016_cov_4.429469"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLD82_02140"
     CDS             <1..991
                     /locus_tag="PLD82_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOG64219.1"
                     /translation="RVSTAQAMDRTLRLGGMDPAAAAQAARAYLDDVARNAGGVRFSI
                     ANSIWHRPNLICLPEFLNLARDTMQAGVHKLDFARPDAADRINDWIADKTENMIKKVL
                     DRIPEDAMMYLVNAIFFHGTWATAFDSDQTKNEAFNKDNGSSQPVPMMRQSGKYRYHA
                     DARGAAVELPYGDGRFAMLAILPAQRGNMRDYLGSLDWAELSRIRQSLRHREGEVRIP
                     RFNLSWGVKDLVDQLKAMGMGLAFSDAADFSGITGKRDLTIDKVLHKAVIEVDEKGTK
                     AAAVTVVGMKATSSMHMDREIPFRFVADRPFVFVIVDTRTGMPVFTGVMHSPA"
     gene            complement(1422..2477)
                     /gene="arsB"
                     /locus_tag="PLD82_02145"
     CDS             complement(1422..2477)
                     /gene="arsB"
                     /locus_tag="PLD82_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017551004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOG64220.1"
                     /translation="MSIQQQGIGFFEKYLTAWVLACMAIGIAIGQFMPEIPSLLSQLE
                     YENVSIPIAVLIWLMIFPMMMKVDFASIKNVGKNPRGLYVTWVTNWLIKPFTMFGMAW
                     LFFFVIFKNLLPPELARDYLAGAVLLGAAPCTAMVFVWSHLSRGNPAYTVVQVATNDL
                     IILVAFTPIVAFLLGVSGLVIPWGTLLFSVGLFVVIPLTAGALTRIHITRRRGLEYFE
                     KTFLPKWNGVTVSGLLLTLILIFSFQGKTILDQPLDILLIAVPLVLQTFLIFYIAWFW
                     ARRWGLPHEIAAPAGMIGASNFFELAVAVAIALYGAKSPVALATIVGVLVEVPVMLTL
                     VRIANAARARSKKRSQA"
     gene            complement(2545..3009)
                     /locus_tag="PLD82_02150"
     CDS             complement(2545..3009)
                     /locus_tag="PLD82_02150"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOG64221.1"
                     /translation="MKRVLFLCTGNSCRSQMAEGWARFFLGNSLEAKSAGIIAKGLDP
                     RAARVMAEAGVDISAQTSKTVSSLIEAHQNQFGQSPDMPPPFDLVITVCGHAHETCPR
                     FPGKVRVIHHGFDDPPHLAEDASSEDEALGHYRRVRDEIRAFVEHKLAALLD"
     gene            complement(3006..3437)
                     /locus_tag="PLD82_02155"
     CDS             complement(3006..3437)
                     /locus_tag="PLD82_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG64222.1"
                     /translation="MNALRTATLCTILILAACGKSDAGNRVPVQSRIVTPPSVGDMLN
                     TIDQFKLTVVELGSVNCRPCKMMTPVLERLSSDYAGTVQVVFFDVWQNQAPAEHYRIQ
                     SIPVQIILDPTGHELYRHVGFIPYDNLKGIIEQLLAKGAKK"
     gene            complement(3434..3664)
                     /locus_tag="PLD82_02160"
     CDS             complement(3434..3664)
                     /locus_tag="PLD82_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG64223.1"
                     /translation="MDIKILGTGCPKCKQLEANAKEAVEKLANGATVEKVGELAKIME
                     YGVMLTPALVVDGVVKSSGKVLSPAEIMEILK"
     gene            complement(3679..4404)
                     /locus_tag="PLD82_02165"
     CDS             complement(3679..4404)
                     /locus_tag="PLD82_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG64224.1"
                     /translation="MEELFIALEKALAGGSLLAALAALAWGMLSIILSPCHLVSIPLV
                     VAFTSNQKTGSAKTGFVFGSLFATGMLITVLCIGVVTAALGRFLGNTGTIGLIGQMVA
                     ALVLLVIGLWLSGLVRLPFLDALTGRGVQARRGKLAAFITGLVFGLSAGPCTFAWMAP
                     ILAITFASAQTDLANAIWLFGWFAIGHGGVLVLAGVFSGAVEKMLHWNNASERTVTII
                     KIILGILIFLGGIAIVIDAIEKF"
     gene            complement(4421..5647)
                     /locus_tag="PLD82_02170"
     CDS             complement(4421..5647)
                     /locus_tag="PLD82_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG64225.1"
                     /translation="MNKQIAIFSAILAVFLAFFFLNLAMPPAEALATDGLAGAFNEAL
                     LMAHDYAREHVLLCLIPAFFIAGAIGNFISAGSVMRYLGTGARRVTAYAVASVSGSVL
                     AVCSCTILPLFAGIYSRGAGLGPASAFLYAGPAINVLAIILTARVLGPELGMARAIGA
                     VGFSIIVGLLMHAFFRKEEAARQQAHAKSFADVAAEIPTRTLAQTAVFFFSLVGILVF
                     INWGPDPASPVWQAIYSAKYWITGYFGLILVMMLWLLFKRNELASWSSATWGFAKQIL
                     PLLLGGVLVAGFLLGRPGHEALIPSDWVASAVGGNGILANLFASIAGALMYFATLTEI
                     PIMQGLMGSGMGNGPALALLLAGPALSLPSMLVIRSILGTKKTLAFVGLVVVLSTLAG
                     LIFGALYPQPFAGPGR"
     gene            complement(5644..5997)
                     /locus_tag="PLD82_02175"
     CDS             complement(5644..5997)
                     /locus_tag="PLD82_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG64226.1"
                     /translation="MQTRTTPLIYEARAEFLKALAHPGRLHLVDLLKDGERCVCELAA
                     ATGYDISTVSRHLAQMKRAGIVSDRRQGTSAYFTLNLCCIADLFTCMEKSLHKRQQSI
                     SAALAACAPETESQA"
     gene            complement(6329..7672)
                     /locus_tag="PLD82_02180"
     CDS             complement(6329..7672)
                     /locus_tag="PLD82_02180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG64227.1"
                     /translation="MPFHQLILAFLTGILFSYSVQSFGKAAIITRSTDKHFWLGLTLL
                     SGSIYITLQLFLTMPSDSETMVSIHRLKILALLFTVTGWIHTFYRISFPSSRYPMIFT
                     ILAGITALFIPFDLFLDLPISSIRTEFLGIPFTHHFATTGPVYLFMSFLILIPFSIIP
                     ILTYILDPNFSRREKAFGCLVFSPGLIGGLNDFAVTNHLYSGIMISEYVFFIFLAGVS
                     IHLFREDASNHRFLKNLKTELEKKIDQRTAELKEANHQLQAMASTDLLTGLCNRREFT
                     HILTQEESRIKRYQGTEPHTFSILFIDLDNFKYYNDTFGHAAGDLVLAGFASLLKGML
                     RTPDTAARYGGDEFVALLPSTGEGGAVALAQRILTELKSLAGFQKEIARLTGQDRTIP
                     VEKQVGCSIGVATYNSTSLNESEAILIAADRALYAAKSAGKHRVKVWRPDDGGHT"
BASE COUNT         1784 a         2015 c         2560 g         1657 t
ORIGIN      
        1 ccgggtttct acggcacagg cgatggaccg caccctgcgt ctgggcggca tggatcctgc
       61 ggccgcggcc caggcggcca gggcctatct ggacgatgtt gcgcgcaatg ccggtggggt
      121 ccggttttcg atagccaact ccatctggca ccggccgaat ctcatctgtc tgccggagtt
      181 cctcaatctc gcaagagata ccatgcaagc cggcgtgcac aagctcgatt ttgcccgccc
      241 tgatgcggct gaccgcatca atgactggat cgcggacaaa accgaaaaca tgatcaaaaa
      301 ggtgctcgac aggatccccg aagatgcgat gatgtacctt gtcaatgcca tcttttttca
      361 tggaacctgg gccaccgcat ttgactcgga ccagaccaaa aatgaggcgt tcaacaaaga
      421 caatggttcg agtcagcctg tccccatgat gcgccagtcg ggcaagtacc gctatcatgc
      481 cgatgccagg ggagctgcgg tcgagctgcc ctatggtgac ggccgctttg ccatgctggc
      541 catcctcccg gcgcagcgcg ggaatatgcg cgactatctt ggctctttgg attgggccga
      601 actgtcacgc atccgccagt ccctgcgtca ccgtgagggc gaggtgcgca tcccccgatt
      661 caatctctcc tggggcgtca aggatctggt tgatcaactc aaggccatgg ggatgggcct
      721 ggcgttttcc gatgcggccg atttttcggg gatcaccggg aagcgtgacc tgaccatcga
      781 taaagtgctg cacaaggccg tgatcgaggt cgacgaaaag gggaccaagg cagccgctgt
      841 gactgtggtt ggcatgaagg ccacgtcctc catgcacatg gatcgtgaga tcccgttccg
      901 ctttgtagct gaccggccct ttgtctttgt catcgtggac acccgcacgg ggatgccggt
      961 ctttactggc gtgatgcaca gccctgcctg atgaataggc aggttgacac cagggtgctc
     1021 tgttttctca aaattccctg cactatggtg tcggagtaca tctgatgtgg caagaaatgc
     1081 accatggtgg gcatgcgctg gccttgaata tgcaccatgg tgttacaatc cataaaaaaa
     1141 catgacacta tggtgccggt ttttctcaaa cctgtaaaat tttgacacca tggtgctgtt
     1201 tacccggccg gttgtgccgg gtatccgact ggcacgtttg gaagacgctg ttttgtgtac
     1261 cctgctgttt tacgctttaa cggtgagatt tttgccagtt ctttagggac ggttcgcgaa
     1321 ccgtccctgc tgcaatccgg atcatgtagg ccaatatgcg caacaggcat ggctttggcg
     1381 gtgcatgctg ctcgaagaac gagattccgg gtgaaacctg gttatgcctg cgatcgtttt
     1441 ttcgatcgcg cccgcgctgc attggcgata cggaccaggg tcagcatgac ggggacttcg
     1501 acgaggacgc ccacgatggt ggccagtgcg acgggtgatt tggctccgta cagggcaatg
     1561 gcgaccgcta cggcgagttc aaaaaaattt gaggcgccga tcatgccggc cggggcggcg
     1621 atttcatggg gcaggcccca gcggcgggcc cagaaccagg cgatgtaaaa gatcaaaaat
     1681 gtctgcagga ccagcgggac ggcaatcagg aggatgtcga ggggctgatc gaggatggtt
     1741 ttgccctgaa agctgaagat caatatcagg gtcaagagga gtccgctgac ggtgacgcca
     1801 ttccatttgg gaagaaaggt tttttcaaaa tactccagac cccgtctccg ggtgatatgg
     1861 atccgggtga gcgcaccggc cgtgagggga atgacgacaa agaggccaac cgaaaaaagc
     1921 agtgtccccc agggtatgac caggccactg acaccgagca gaaaagcaac gatgggcgta
     1981 aaggcgacca ggatgatcag gtcattggtg gcgacctgga cgacggtgta ggccggattt
     2041 cccctggaga gatggctcca gacaaagacc atggcggtac agggtgcggc ccccaagagc
     2101 acggctccgg ccagatagtc ccgggcgagt tcgggcggga gcaggttttt gaagatgacg
     2161 aaaaaaaaga gccaggccat accgaacatg gtgaagggct tgatcagcca gttggtgacc
     2221 caggtcacgt acagcccccg ggggttttta cccacgtttt tgatgctggc aaaatcgacc
     2281 ttcatcatca tggggaagat catcagccag atcaggacgg caatggggat cgagacgttt
     2341 tcatactcaa gctgtgacaa taatgagggg atttcgggca tgaattgccc gatggctatc
     2401 ccgatggcca tgcaggccag gacccaggcc gtcagatatt tttcaaaaaa tcctattcct
     2461 tgttgctgta tactcatgat ttcccccctg ttcggtgtga atggtatgga ttgcggccgc
     2521 ttgtatccgg ggctatgtcc agaatcagtc gagcaaggcg gccagcttgt gttcgacaaa
     2581 ggcgcggatc tcgtcgcgga cacggcgata gtggccaagt gcttcatcct cgctggacgc
     2641 atcttcggcg agatggggtg ggtcgtcgaa gccatgatgg ataacgcgca cttttccggg
     2701 aaatcggggg caggtttcat gcgcatggcc acagacggta atcacaagat caaagggggg
     2761 tggcatatcg ggtgactgcc cgaattggtt ttggtgtgct tcgatcaggc tgctgacagt
     2821 tttggatgtc tgtgcgctga tgtcaacgcc ggcttcagcc atgacccgcg ctgcgcgcgg
     2881 gtcgagtccc ttggcgatga tgcccgccga cttggcctcg agactgttcc ccaaaaaaaa
     2941 tctggcccag ccctcggcca tctggctgcg acaggaattg cccgtgcaga gaaagaggac
     3001 ccgcttcatt ttttggcccc cttggcgagc aattgttcga taatgccctt gaggttgtcg
     3061 tagggaatga agccgacatg gcgatagagc tcgtgcccgg tggggtcaag gatgatctgg
     3121 acggggatgg actggatgcg gtagtgttcg gccggggcct ggttttgcca gacatcgaaa
     3181 aaaacgacct ggactgtgcc ggcgtagtcg ctggagagcc gttcaaggac cggggtcatc
     3241 atcttgcagg ggcggcagtt gaccgagccg agctcgacga cggtcagttt gaattggtcg
     3301 atggtgttga gcatgtcgcc caccgaaggc ggggtaacga tgcgggactg gaccgggacc
     3361 cggttgccgg cgtcgctctt gccacaggcg gcaaggatga ggatggtgca gagtgtggct
     3421 gtgcgcagtg cgttcatttg aggatctcca tgatttctgc gggcgagaga accttgccgc
     3481 ttgatttgac gacgccatcg acgacgaggg ccggggtcag catgacgcca tactccatga
     3541 tctttgccag ttcgccgact ttctcgactg ttgcaccgtt ggccagcttt tcgactgcct
     3601 ctttggcatt ggcctcaagc tgtttgcact tgggacagcc tgtcccgaga atcttgatat
     3661 ccattgcagc tccttttttc agaatttttc gatggcatcg atcactatgg cgatgcctcc
     3721 gaggaagatc aatattccaa gaatgatttt aataatggtt acagtgcgct cgctggcgtt
     3781 gttccagtgc agcatctttt caacagctcc tgagaacact ccggccagga cgaggacgcc
     3841 tccgtggccg atggcaaacc agccaaagag ccagatggcg ttggccagat cggtctgggc
     3901 gctggcaaag gtgatggcca ggatgggtgc catccaggcg aaggtacagg ggccggctga
     3961 gagtccgaag accagtcctg tgataaaggc agccagcttg cctcgtctgg cctgtacgcc
     4021 gcgaccagtc agcgcatcaa gaaagggcaa gcgcacgagt ccgctcagcc agagtccgat
     4081 gacgagcagg acaagggctg caaccatttg accgatcagg ccgatggtac cggtattgcc
     4141 cagaaagcga cccagggcgg ctgtgacgac cccgatgcag aggaccgtta tcagcatgcc
     4201 tgtcgcaaag agactcccga agacaaaccc tgttttggct gagcctgtct tctggttgct
     4261 ggtgaaggcg acgacaaggg ggattgaaac gaggtggcag ggtgagagga tgatcgagag
     4321 catgccccag gcaagcgctg ccagggctgc caggagactt ccaccggcga gggccttttc
     4381 gagggcgatg aaaagttctt ccataaaatt ttggctcctg ttatcggccg gggccggcaa
     4441 acggttgggg atagagtgcg ccgaagatca gaccggcgag ggtggagagg acgacaacga
     4501 gtccgacgaa ggccaaggtt ttttttgttc caaggatcga gcggatgacg agcatcgagg
     4561 ggagggagag tgccgggccg gccaaaagga gggccagggc cgggccattt cccatcccgc
     4621 tccccatcag cccctgcatg atggggattt cggtcagggt ggcgaagtac atcagggcgc
     4681 cggcaatgga agcgaagagg ttggccagga tgccgttgcc accgacagcg ctcgcgaccc
     4741 aatccgacgg aatcagggcc tcgtggcccg gtcgaccaag caggaagccg gccacgagga
     4801 cgccacccag cagaaggggc aggatctgct tggcaaaacc ccaggtggca gatgaccagg
     4861 aggcgagctc gttacgcttg aagaggagcc agagcatcat taccagaatc aatccgaaat
     4921 agcccgtgat ccagtacttg gccgagtaga tggcctgcca gacaggtgag gctggatccg
     4981 gtccccagtt gatgaagacc aggatcccga ccagcgagaa gaaaaagact gcggtttggg
     5041 cgagtgtccg cgtggggatc tcggccgcca catcggcaaa tgacttggcg tgtgcctgct
     5101 ggcgtgcagc ctcttctttt ctgaaaaagg cgtgcatgag caggccgacg atgatggaaa
     5161 aaccgacagc cccgatggcg cgggccatac ccagttcggg tccgaggacg cgggctgtca
     5221 ggatgatggc gaggacgtta atggccgggc cggcgtataa aaaggcgctg gccggcccaa
     5281 gaccggcccc gcgtgagtag atgccggcaa agagcggcag gatggtgcag gagcagacgg
     5341 ccaacacaga acctgaaact gaagccacgg cataggctgt cacccgcctg gctccggtcc
     5401 cgagatagcg catgacactc ccggcgctga tgaagtttcc gatggccccg gcgataaaaa
     5461 aggcggggat caggcagagc aggacatgct cgcgggcata gtcgtgtgcc atcaggaggg
     5521 cttcgttgaa ggcgcctgcc agtccgtctg tggccagtgc ttcagccggg ggcatggcaa
     5581 ggttgagaaa gaaaaaagcc agaaacacag cgaggatggc cgaaaagatg gcaatctgtt
     5641 tgttcatgct tgactctccg tttcgggtgc acaggctgcg agagctgcgc ttatgctttg
     5701 ctgccgcttg tgcaggctct tttccatgca ggtaaagagg tccgcgatac agcagaggtt
     5761 gagcgtgaaa taggccgagg tcccctggcg gcggtcgctg acgataccgg cgcgtttcat
     5821 ctgggcgaga tggcgcgaga cggtcgagat gtcgtatcct gtggcggccg ccagctcgca
     5881 gacgcagcgt tcgccgtcct tgaggaggtc aacgaggtgg aggcgacccg ggtgggccag
     5941 ggccttgaga aactcggccc gggcttcgta gatgagaggt gtggttcgtg tttgcatact
     6001 tgcaaaatac tgcaaatatg caagcaaggc aagcaaggcc cttgcgctca agagcatcgg
     6061 ccatccggag tatccggaaa cgggagcacc atggtgtcga aaacaggtta caaaaagtgt
     6121 atgtatggga tttgagtgac gggtgcctgg atcatgggga tggtccagct ttgccgttca
     6181 tggtgcaggc ttgtactatg atgcagggac agtgcgtttc cggccgatca ggcacgatgg
     6241 tgtcggaaat ggttttctgt gcaccatggt gccgggaatt cttctcctga tacattgtat
     6301 gatacgtcca gtcgcgggat gtccatggtc aggtgtgtcc gccgtcatcc gggcgccaga
     6361 ctttgacgcg gtgttttccg gctgacttgg ccgcatagag agctctgtcg gcggcaatca
     6421 ggatggcctc gctctcgttc aagctggttg agttgtaggt ggcaacgccg atggaacatc
     6481 caacctgttt ttcgacaggg atggtacggt cctggcccgt caaccgggca atctcttttt
     6541 ggaaaccggc aagagacttg agttcggtca ggatgcgttg ggccaatgcc acggcaccac
     6601 cttctccggt gctcgggaga agcgccacga attcatcccc gccgtagcgg gcagccgtgt
     6661 cgggtgtgcg gagcatgcct ttgagaaggg aagcaaatcc ggcaagaacc aggtccccgg
     6721 cagcgtgccc gaaggtgtcg ttgtaatatt tgaaattgtc gagatcgata aagaggatcg
     6781 agaacgtgtg cggctctgtt ccttggtagc gtttgatccg ggactcttcc tgtgtcagga
     6841 tgtgggtgaa ttcccgtctg ttgcagagtc ctgtcaggag gtcagtggac gccatggcct
     6901 gcagttggtg attggcttcc ttgagttccg cggtgcgttg gtcgatcttt ttttcgagtt
     6961 cggttttcag attcttcagg aaacgatggt tggacgcatc ttcccggaag agatggatgg
     7021 agacaccggc aaggaagatg aagaaaacat attctgaaat catgatcccg ctgtagaggt
     7081 ggttggtcac cgcaaagtca ttcaatccgc cgatcagccc cggagagaag acaaggcacc
     7141 cgaaggcttt ttcccggcgt gaaaaattgg gatcaaggat gtatgtcaga atcgggatga
     7201 tggagaaggg tatcagaatg agaaaagaca tgaagagata gactgggccg gtcgtcgcga
     7261 aatgatgggt gaaaggaatt cccaggaatt cggtccggat ggaactgatg ggcagatcaa
     7321 gaaacaggtc gaaagggatg aagagagccg taatgccggc gagaatggta aagatcatgg
     7381 ggtagcggct ggacggaaac gagatgcggt agaaggtatg gatccagccg gtcacagtga
     7441 aaagcagggc gaggatcttg aggcgatgga tggaaaccat ggtctcgctg tcggatggca
     7501 tggtcaaaaa cagctgcaag gtgatataga tcgaaccgct caggagtgtc aaaccgagcc
     7561 agaagtgttt gtccgtgctt ctcgtgatga tcgcggcctt cccgaaggac tggacgctgt
     7621 agctgaacag gattccggtc agaaaggcca gaatgagctg atgaaacggc atgcgaatct
     7681 ccaaagtcga actcgaagag gactggacag ggtggtcgct actcccgata ttttggcaca
     7741 tccacccggt tgacgcaata tgtctgtcgg tgaaccttgc agcaatgtga tactcttcga
     7801 tattccggag ggagagttgg tcggtgcaaa cgtgtccgat cctggcaccc ctcactggcc
     7861 tgaggatgtt catcatgaaa aaccactgat cgacgaagca gtgacaggga atgaaactca
     7921 gtgacaggga gcgcgtctct tggtaggata ccagaggaat ggaggtgtca cgatggcagt
     7981 tcttgaggca ttgaaggaag cgatcctttc ggagcg
//
LOCUS       DAOSHE010000042         7992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7759_length_7992_cov_4.309185, whole genome shotgun sequence.
ACCESSION   DAOSHE010000042 DAOSHE010000000
VERSION     DAOSHE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7759_length_7992_cov_4.309185"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2163)
                     /locus_tag="PLD82_02185"
     CDS             complement(<1..2163)
                     /locus_tag="PLD82_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG64228.1"
                     /translation="MFLKNLKIYGFKTFGRKVVVDFQKGITAIVGPNGAGKSNLIDAL
                     NWVTGETRLSDLRVRTSEQLIFHGSPSLKPLSLAEVSLTIENTDNLLPIEYAEVNITR
                     RIYRDGTSEFFINKNKCILKDITNLFLGTGAGRGAYASMRQGEIDRILKQKPEERREI
                     FEEAAGILKYRNKRRETERDLERAESNLKQIRPTLLEVERLYHAKKKQAEKAERYNKL
                     MDRRIAVEVDIHLVKVFDLKRELESKNEVLQKQLDKKQTLLDKLKTIEMDIDASLAQS
                     QDLQQNQHSTQTEILKHETEISALRQRIASLGDRKMALELNIKEWDAALLQSDQRLAK
                     IDSILEDLAKEKSNLAGMIAERQDNLEQYARDITAIQKIVDDDTANIAAYHKKIADLD
                     IALAESRQRLEEVINRMVEAIDKRKAELKGSAELKQDLKTSIARALDELGVFLKGRKD
                     VLSDLASLDITRHSDKEKVKSTLAALRDSFEQRLASVEHLRQQFSQLDNLISGFDEII
                     FAREGIHAQKESLDKAIGDMQRDEKAHKERIVFLETDIRNQQARIETIKQMMHDSQLA
                     LVQMREKSRAVEESMKTQQGFRRDVEKQREGVAASIRQARETIAGIDTESAGLVKDQV
                     RVVTSQDKLKADLARISKNLSSITQASSGKEKKARECKEQLEEFHARIEEATRSITTI
                     EVEMRTIYENFYENYSIDLKEHEKEIRNRKFDVGELRTE"
     gene            2301..3101
                     /gene="mazG"
                     /locus_tag="PLD82_02190"
     CDS             2301..3101
                     /gene="mazG"
                     /locus_tag="PLD82_02190"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOG64229.1"
                     /translation="MDARLEAFHQVLELVTRLRGPDGCPWDREQTLESITPFILEECH
                     ELLEALARGDKPGICEESGDLLFQIVFLAQLEEEAGHFGMREILERIHAKMVSRHPHV
                     FGDAAVHDVETVLTNWERIKAGEKDKKGRASILDGVPSSLPSLARAMALQQKAARVGF
                     DWTNPEDVLAKIEEEIRELREAESKQDKAAIREEFGDILFALVNLARKSGIDPEHSLT
                     MTTEKFIRRFRHIETRAREESLVLTECSLETMDAWWDEAKNIERGCKA"
     gene            complement(3105..3833)
                     /locus_tag="PLD82_02195"
     CDS             complement(3105..3833)
                     /locus_tag="PLD82_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64230.1"
                     /translation="MKIRFVLLLLILPCAATASATWRGHALMPGTDFATWYYIADTGK
                     PGPRILIFAPHSDEQSAGEYLESFVKTLVPDRGFVIACPWPVAPARRINSRLYIEDIN
                     RQFGSRRDNYTAIDLLSHHVRRWMELYNINFVLNLHEGWKQFRNDWNEYGQSFFIDIP
                     ELIPMAERVVGRVNLRIWAQNRFVVVQQPMPDTLTWYCATRGVPAFGIEIERGLPLQT
                     KIVYQRIVLEEFFRELGIRTVQRR"
     gene            complement(3830..5584)
                     /locus_tag="PLD82_02200"
     CDS             complement(3830..5584)
                     /locus_tag="PLD82_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64231.1"
                     /translation="MKRSARPVCTLILAGMILFLGGCGSSYYAKAVEYRETGRLDEGI
                     AWVREKLRDESGALDMFDAHRVVLQFFEALDRLDEGFALYARFRDPALTNYIAGLKFQ
                     HEGMHVSATNAFNRALKIRPDEYAIWYDLAVSLTRMGKYKEARQILGRVYRLNDSFAP
                     AIYDMGVLYGYGRNEPAAGMSWLVEALDTYYPFEKNAILDARITLGLFSELSGEWARA
                     RDTYEACGPENFARVIRTGDPGEAWARTGNIAHARELWQKALDILGYQNARGRHFFKK
                     FHGSRQGLVDFSEIKFKYATIESRHEDLPRFVFASEQEPVFLTNTWGPMHAVFQKDGM
                     SDLHFGWDTWYVEYVGILSRPQPVQAWIQTRGVVSMTNTRDGLRAYVSLQKSSFDVQV
                     PDRENPGSYKTETFWREGNPKIGLYQGTRLVANFSPVMMHFHSLVVADVDGDGLDDLV
                     SCGFDGAGQLVLDIRVRSGGNWKALAPMTCGLENPANGFVLLDLDNKPGMELVTFSGL
                     ISWADVHVLGQHGYQKNNLVFPEFARDFSRRYSFLDQQKINERLYSDKRTAEEKAFVT
                     AIGQYRSIADGIVRESGQ"
     gene            complement(5645..7036)
                     /gene="murC"
                     /locus_tag="PLD82_02205"
     CDS             complement(5645..7036)
                     /gene="murC"
                     /locus_tag="PLD82_02205"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG64232.1"
                     /translation="MLKNNIKKLHFIGIGGIGMSGIAEIFHNLGFPVSGSDLAESRIT
                     KRLASLGITIHLGHNASNVADADVVVYSAAVPPTNPEIDFARSKKLPLIPRAEMLNEL
                     TRLKTGIAISGTHGKTTTTSMVAEIFAEAGLDPTYVIGGKLNSINSNARLGKGEHIIF
                     EACEAFGSFQYFSPIAMVLLNIDSDHLEYYETMDSLRNAFLSFVNKIPFYGMAIVNND
                     DPNVRMILDRVSKRIVSYGIDSESDYMAKNLQFHDWSSSFDLVANGTNLGRIKLILPG
                     KHNVSNAVAATAVALENGISFDAISRALGRFVNADRRFQIKGEYRGITVVDDYAHHPS
                     EIRATLEGARQHPGKRIIAVFQPHLFSRTLQLYADFATALSIADEVILLPVYPAREKP
                     IPGVTSQLVVDAMGKAGYSSCILHEDPASLSKLLLERTREKDLVLFLGAGSIWQYAEE
                     FVELLAREEARYG"
     gene            complement(7114..>7992)
                     /gene="aroB"
                     /locus_tag="PLD82_02210"
     CDS             complement(7114..>7992)
                     /gene="aroB"
                     /locus_tag="PLD82_02210"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOG64233.1"
                     /translation="KTRETKSMLEDAMFHNQHDRKTLVIALGGGVTGDLAGYLAATYM
                     RGVPFVQIPTSLLAQVDSSVGGKTGVDVPAGKNLVGAFWQPSGVYIDPNLLSTLPERE
                     YLSGLGEIVKHALMFSPEMIHLLEHGQHPIMERDPLMMADLVAKNCTIKAGVVSADET
                     ESGLRRTLNFGHTVAHAIELASGYSVSHGVAVMAGILVEVRAAVLSGLLSEESATAMG
                     TTVMRYLHNPPGIQNFDPDTMVQAMLLDKKNTEGAIHCSLPEAPGIPPDCTWTRAFSE
                     EILKAALRDVFLPTGG"
BASE COUNT         1852 a         2157 c         2083 g         1900 t
ORIGIN      
        1 ctcggtacga agttcgccca cgtcaaactt gcggttgcgg atctcttttt cgtgttcctt
       61 gagatcgatg ctgtaattct cgtaaaagtt ctcgtagatt gtacgcatct ccacctcgat
      121 cgtggtgatg ctccgtgtgg cctcttctat cctggcatgg aactcttcca gttgttcctt
      181 gcactcccgt gcttttttct ctttgccgga ggatgcctgg gtaatcgaag aaaggttttt
      241 ggagatcctt gccagatcag ccttgagctt gtcctgactg gtgacaaccc ggacctgatc
      301 cttgaccagg cctgcactct cggtgtcaat cccagcaatg gtctcccggg cttgacggat
      361 gctggcagcc accccttcac gctgtttttc gacatcccgt ctgaaaccct gctgtgtctt
      421 catgctctct tcaacagccc ggctcttctc gcgcatctgg acaagggcga gctgggagtc
      481 atgcatcatc tgcttgatcg tctcgatcct ggcctgctgg ttccggatgt cggtttcaag
      541 aaaaacgatc cgttccttgt gtgccttctc gtcacgctgc atgtccccga ttgccttgtc
      601 gagggactcc ttctgggcgt gaatcccttc gcgggcaaaa atgatctcgt caaatccaga
      661 aatcagatta tccaattgag aaaattgctg ccgaaggtgt tcaaccgaag caagccgctg
      721 ttcaaaactg tccctaagcg cggccagggt gcttttgact ttttctttgt cactgtgtcg
      781 cgtgatgtca agggatgcaa gatcggagag gacatccttg cgccccttga gaaacacccc
      841 aagctcgtca agagcacggg caatgctcgt cttgagatcc tgtttaagtt ccgcagagcc
      901 tttgagttcg gctttgcgct tatcgatggc ttcaaccatg cggttgatga cctcttcaag
      961 acgctgccgg gattcagcaa gagcaatatc gagatcggca atctttttgt gataggccgc
     1021 gatattggcc gtgtcatcat cgacaatttt ttgaatggct gtgatatccc gggcgtactg
     1081 ctcaagattg tcctgccgct cggcgatcat ccctgcgaga ttcgattttt ccttggcgag
     1141 atcctcgagg atggaatcga tcttggccaa cctctggtcg ctctgcagga gcgcagcatc
     1201 ccattccttg atattcagtt ctagggccat cttccggtcg cccagggagg caatcctttg
     1261 gcggagggca ctgatctcgg tctcgtgctt gagtatctct gtctgcgtcg agtgctggtt
     1321 ttgctgcagg tcctgactct gtgcaagcga ggcatcaatg tccatctcga tggtcttgag
     1381 cttgtcaagc agggtctgct ttttgtcgag ctgtttctga agaacctcgt ttttactctc
     1441 gagctcgcgc ttgagatcga acaccttgac aagatggata tcgacttcga cggcaatccg
     1501 ccggtccatg agcttgttgt agcgctctgc cttctcggct tgctttttct tggcatgata
     1561 gagccgttca acctcaagca gtgttggacg gatctgcttg aggttggact cggccctctc
     1621 gaggtcccgc tcggtctcac gacgtttatt ccggtatttg agaatgcctg cggcttcctc
     1681 aaaaatctcc cggcgttctt caggtttctg cttgaggatt ctgtctatct cgccctgtcg
     1741 catggaagcg taggctccac ggccggcacc tgttcccaga aaaaggtttg tgatgtcctt
     1801 gagaatgcac ttgtttttat tgatgaaaaa ttcactcgtc ccgtcccggt aaatcctgcg
     1861 cgtgatattg acctcggcgt attcaatggg aaggagattg tccgtgtttt cgatggtcag
     1921 agagacttca gccagggaga gcggcttgag ggatggcgat ccatgaaaga tcaattgttc
     1981 tgatgtccgg acacgcagat cagaaagtct ggtctcaccg gtcacccagt tgagggcatc
     2041 aatcagattg gacttccctg ctccgtttgg tccgacaatg gccgtgatcc ccttttggaa
     2101 atcaacaacg accttgcgcc cgaaggtctt gaaaccatag atcttgagat tcttgaggaa
     2161 caagggagca tcctgtcatg gaaaggtggc attactcata tctatataaa gatactcacg
     2221 aaggaagcat caagacaagg gagcacgtgg gcggggaatg atggtatctt tgggtgagtc
     2281 tacaacatat ggagacagat atggacgccc gtcttgaagc attccatcag gttcttgaac
     2341 tcgtcacacg gctacgaggc ccagatggat gtccctggga cagggaacag acgcttgaaa
     2401 gcatcacccc tttcatcctt gaggaatgcc atgagcttct tgaggccttg gcccgtggag
     2461 acaaaccggg gatatgcgaa gagagcggcg acctgctttt tcagatcgtc tttctggctc
     2521 agcttgaaga agaggctggc cacttcggga tgcgggagat cctcgaacga atccatgcca
     2581 aaatggtctc gcgtcaccca catgtattcg gtgatgcagc cgtgcatgat gtcgaaacgg
     2641 tcctcaccaa ctgggagagg atcaaggcag gcgagaagga caaaaagggc cgggcatcca
     2701 ttctggatgg cgtcccgtct tccctgccat cactggcgcg tgccatggct ctccagcaga
     2761 aagccgctcg cgtcggtttt gactggacaa atcccgaaga tgttctggca aagatcgagg
     2821 aggaaatccg ggaactccgt gaggccgaat cgaagcagga caaggcagca atccgcgagg
     2881 aattcggcga cattctcttt gctcttgtca atcttgcccg aaaaagcggg atcgatcccg
     2941 aacactccct gaccatgaca acggaaaaat tcatccgccg cttccggcat atcgagacac
     3001 gagcccggga ggaatccctt gtcctgacgg aatgcagcct ggaaacgatg gatgcatggt
     3061 gggacgaagc gaagaacatc gaacgtggct gcaaggcctg agccctatcg tctctggaca
     3121 gtcctgatcc ccagttcacg gaagaattct tcaagaacga tgcgctggta gacaattttt
     3181 gtctgaagcg ggagaccccg ttctatttcg atgccaaacg cgggcacacc ccttgttgcg
     3241 caataccacg tgagggtatc cggcatgggc tgttgaacga ccacaaaacg gttttgggcc
     3301 cagatccgta aattcacgcg accaacaacc cgttccgcca tcggaatcag ttccggaata
     3361 tcgataaaaa aactctggcc atactcgttc cagtcattcc ggaactgttt ccatccttca
     3421 tgcagattga gaacgaaatt gatgttgtag agttccatcc agcgtctgac atgatgggaa
     3481 agaagatcga ttgcggtata gttgtcccgt cttgatccga actgcctgtt gatatcttca
     3541 atgtagagtc gtgaattgat ccttctggcc ggggccaccg gccagggaca ggcaatcaca
     3601 aaaccacgat ccgggaccag ggttttcaca aaggattcga gatactcacc ggctgactgc
     3661 tcatcactgt ggggagcaaa aatcaggatc cgggggcccg gttttccggt atcggcaata
     3721 taataccagg tcgcaaaatc ggttcccggc atgagcgcat gcccacgcca ggttgcagaa
     3781 gctgttgctg cacatggaag gatcaagagc agcaacacaa accggatctt cactgtccgc
     3841 tctcccggac aatcccgtcg gcgatgctcc ggtattgtcc gattgccgtc acaaaggcct
     3901 tctcttccgc tgtccgtttg tcggaataca gacgctcatt gatcttctgt tggtccagga
     3961 aggaatagcg tctgctgaaa tcccgggcaa attcggggaa gaccagattg ttcttctggt
     4021 atccatgctg gccgagaaca tgcacatccg cccaggaaat caacccggaa aaggtgacaa
     4081 gctccatgcc gggcttattg tcaagatcga gaagaacaaa cccgttggcc ggattctcga
     4141 gtccacaggt catcggggcc agggccttcc aattcccgcc ggatcgcacc cgaatgtcaa
     4201 ggacgagctg accagctccg tcaaaaccgc atgaaacaag gtcatccaac ccatcaccgt
     4261 cgacatccgc cacgaccaga ctgtggaagt gcatcatgac cggagaaaag tttgccacaa
     4321 gacgggttcc ctggtagagt ccgattttcg gattcccctc acgccagaat gtttctgttt
     4381 tataggaacc aggattctcc cggtcaggga cttgcacgtc aaacgatgat ttttgcaagg
     4441 agacataggc ccgcaaaccg tccctggtat tggtcattga aaccacgccc cgtgtttgga
     4501 tccaggcctg taccggttgt ggtcgggaca agattccgac gtattcgaca taccaggtgt
     4561 cccatccgaa atgaagatca ctcatgccat ctttctgaaa aacagcatgc atgggccccc
     4621 aagtatttgt caggaaaacc ggttcctgct cggacgcgaa gacaaaacgg ggaagatcct
     4681 catgccgtga ctcgatggtc gcatatttga acttgatttc ggagaaatcg acgagccctt
     4741 gtcgtgaccc atggaacttt ttgaaaaaat gccgtccacg ggcattctgg tatccgagaa
     4801 tatccaatgc tttttgccag agttcccggg catgagcgat attcccggtg cgggcccatg
     4861 cttcacccgg atctccggtc cggattaccc tggcaaaatt ctcgggaccg caagcttcgt
     4921 aggtatccct ggcccgtgcc cattctccgg agagttccga aaaaaggcca agagtgattc
     4981 gggcatccag aatggcattc ttttcgaatg gataataggt gtcaagagct tcgacaagcc
     5041 aggacatccc ggctgccggt tcattccggc catacccgta aagaacgccc atatcgtaga
     5101 tggccggggc aaaggaatca ttcagcctgt agaccctgcc gaggatctgg cgagcttctt
     5161 tgtactttcc catcctggtc agggaaaccg ccagatcgta ccagatggca tactcgtccg
     5221 gacggatctt gagcgcccgg ttgaaggcat tggtcgcaga gacatgcatc ccttcatgct
     5281 ggaacttcaa tcccgcgata taattcgtca acgccggatc gcggaaccgt gcatagagag
     5341 cgaatccctc gtcaagtctg tccagcgctt cgaagaactg cagaacgaca cggtgtgcat
     5401 caaacatgtc caaagcgccc gattcatctc gcagtttctc ccgaacccag gcgattccct
     5461 catcaagcct gccggtctca cggtactcga cagccttggc ataatacgaa gaaccgcaac
     5521 ctccaagaaa caggatcatg ccagccagaa tcagggtaca cacaggcctt gcggatcgtt
     5581 tcattgtcag actcctttgt atcaggtact ggacaggcag agaccggcct gtccggaaac
     5641 atggtcaacc atagcgggct tcttcccgtg ccaggagttc gacaaactct tcggcatact
     5701 gccagatgct tcccgcaccg aggaacagaa ccagatcttt ttccctggtc cgttcaagga
     5761 ggagtttcga aagtgaagcc gggtcctcgt ggaggataca ggaagaatag ccggcttttc
     5821 ccatggcatc aacaaccaat tgacttgtga cacctggtat cggtttttca cgggccgggt
     5881 agaccggaag gagaatcacc tcatccgcaa tcgacagggc agtggcaaaa tcggcataca
     5941 actgcagggt gcgtgaaaaa aggtggggct gaaagacagc gatgatccgt tttcccgggt
     6001 gttgcctcgc cccttcgagt gtggccctga tttcggatgg gtgatgggcg taatcgtcaa
     6061 caaccgtgat cccgcgatac tctcccttga tctggaagcg tctgtccgca ttgacaaacc
     6121 ggcccagcgc ccgcgatatg gcatcaaagg aaataccgtt ttcaagggca acggcagtag
     6181 cggcaactgc gttgctcaca ttgtgctttc cgggaagaat cagcttgatc cggccaagat
     6241 tggttccatt ggcaacgaga tcgaacgacg aggaccagtc atggaactgg agattcttgg
     6301 ccatataatc gctctcgctg tcgatcccgt aactgacaat ccgctttgaa acacggtcaa
     6361 gaatcatcct gacattggga tcatcgttat tgacaatggc catcccgtaa aacgggatct
     6421 tgttgacaaa ggaaagaaaa gcattccgga gactgtccat cgtttcgtaa tactcgagat
     6481 ggtccgaatc aatattgaga agaaccatgg caatcgggga gaagtattga aacgaaccga
     6541 aggcctcgca ggcctcaaag atgatatgtt cccctttgcc gaggcgggcg ttgctgttga
     6601 tactgttgag tttcccgccg atgacatagg tcggatcgag accagcttcg gcaaatatct
     6661 cggccaccat cgaggttgtc gttgttttgc catgggtccc gctgatggca atcccggtct
     6721 tgagcctggt caactcattg agcatctccg ccctgggaat caaggggagc tttttggacc
     6781 gtgcgaaatc aatttcggga tttgtcggag gaacggcagc ggaatagaca acaacatcag
     6841 catccgcaac attggatgcg ttgtgcccga ggtgaatggt aatcccgagg gaagccaacc
     6901 gctttgtgat gcgggattca gccagatcgg atccggacac gggaaaaccg aggttatgaa
     6961 aaatttcggc tatcccggac atgccaatcc cgccaattcc gataaaatgc aactttttga
     7021 tattgttttt caacattctt caaccctgtc tgcttccagt caatccttca aatataccta
     7081 taggttgctt cgcttcctat tgcttcgaga gattcaaccc cctgtcggga gaaagacatc
     7141 ccgcaaggcc gccttgagga tttcttccga aaaagccctg gtccatgtgc aatcaggagg
     7201 gataccggga gcctccggca gggaacagtg aatcgcccct tccgtatttt ttttgtccag
     7261 gagcatggcc tggaccatcg tatccgggtc aaagttttga atcccgggcg gattgtggag
     7321 ataccgcatc acagtcgtcc ccatggcagt cgcggattct tccgagagca gtccggaaag
     7381 aacagccgcc cggacctcca caagtatccc cgccatgact gccacgccat gggatacaga
     7441 gtaccctgaa gccagttcga tggcatgcgc aaccgtatgc ccaaaattca aggtccttcg
     7501 caaaccgctt tccgtctcgt ctgctgaaac aactccagcc ttgatcgtac agttcttcgc
     7561 caccagatcg gccatcatca ggggatcccg ctccatgatc ggatgctgcc cgtgttcgag
     7621 caggtggatc atttcgggcg aaaacatcag ggcgtgcttg acgatttcac caagacctga
     7681 aaggtattcc ctttcaggaa gagtcgagag gaggtttgga tcgatataga caccagatgg
     7741 ttgccaaaag gccccgacga gattctttcc cgccggaaca tcaaccccgg tctttccgcc
     7801 gacactggag tcaacctggg ccaaaagtga cgtcggaatc tggacaaagg gcactccccg
     7861 catataggtt gcggccagat aacccgcaag atctcctgtg accccaccgc ccagtgcgat
     7921 cacaagtgtt ttccggtcgt gctgattgtg gaacatcgca tcttcaagca tcgacttggt
     7981 ctctctcgtc tt
//
LOCUS       DAOSHE010000043         7909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_7898_length_7909_cov_3.845047, whole genome shotgun sequence.
ACCESSION   DAOSHE010000043 DAOSHE010000000
VERSION     DAOSHE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7898_length_7909_cov_3.845047"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLD82_02215"
     CDS             <1..256
                     /locus_tag="PLD82_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64234.1"
                     /translation="DAGLAMHRLGLLSSGAAATNVPVSPVVRIDDDYARGIQRAVLEN
                     TIHTIILPWFERVPGNDFLAEDLSSRIRQTASHTDPPDPP"
     gene            162..407
                     /locus_tag="PLD82_02220"
     CDS             162..407
                     /locus_tag="PLD82_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG64235.1"
                     /translation="MNGSRETIFSRRISRPGSGKQRPIQILLILLDNSEADTHIANLA
                     LLAHMLADKELTQALIDTDNPAAIRTLIARALSHSPA"
     gene            484..2970
                     /locus_tag="PLD82_02225"
     CDS             484..2970
                     /locus_tag="PLD82_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG64236.1"
                     /translation="MAEEQPDQQRPLETVLEDLGAAVFDISAIARSPSSAVFSLRRHS
                     PTAPREALKILTIHEASALEIPTGKIHPGILPVSASGQSKTPAGRIFWCLMPWLENSR
                     TLREALLARLEATESSQTTSRILVWLTERLDEAAAALTALHTRGLAHLDIKPDNMLIQ
                     DDGRLVIADTASCRALRGKDQCGITLTRYFAHPDLRTLLSDAAAQDRQALILPRNKLS
                     FIHDIYALGRSILDLLTLLDTRHPGSLPYDPVFSWLHLAACRMLDGHNLPASGPDGLS
                     EIWPGLDREELALLRYRSMEAVSSDFAKLTGRSRPEDDIPELASTGGRRIMVSPGRPA
                     PLTDRVRAIIEHPVFSRLASVPQIELLSSIFPTATHTRYEHSLGAFRNAVRYLDSLLH
                     DIHTPLFRQLVDTRHLTILLLGTLVHDLGQYPFGHTIEELGEHFRHETLSRDFLDNPT
                     RDSSGRTIREIIEDPEHGWGIPLADLKRFLSTPSGREDLFDERRPVDEMLHSILDGPI
                     DVDKLDYLVRDSRMCFLNYGDAIDSERLITNLTIVIQHEQDANGRETRRLCLGAYERG
                     EPAAEALSFVRYLLYQTLYWHHASRAGRTMFKAALAPLVTASGKRPARGSRYHPFMAD
                     IARLLGVAEEPGPITLSDVLDHFEKKADEPGRELIALIRARRYYKRICTIHEGSGSRI
                     QIEDFRNATRKPGFDETLRKLLRARFESRLTSRQGPGASLLTPDRSGPALEILSRQRT
                     ILCDAPRPSIGSHSKLHFVPEPQRLERNHSERHLSGERVSAVWNDVHARLMSIAAKGR
                     VFCHPDLREALLAALGPAGVEDCILEAMKK"
     gene            3086..3628
                     /locus_tag="PLD82_02230"
     CDS             3086..3628
                     /locus_tag="PLD82_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG64237.1"
                     /translation="MSPTVKLGPRTLPAESYAKLDTYIDSLGITKQDPRKKGFLIQIL
                     HKAQHIFGHLPEEVQKHVANRLWLHHADVSGVISFYNYFTTIPKGKISISICMGTACY
                     VKGADKILAEFEKQLGIQAGGLTDDGLFSLDVLRCVGACGLAPVVLVNEKVYGRLKPE
                     DVSRVITEARTMHTAPEAAP"
     gene            3625..5538
                     /locus_tag="PLD82_02235"
     CDS             3625..5538
                     /locus_tag="PLD82_02235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG64238.1"
                     /translation="MTRIDSFEALRTMVRESASLLDLRHHAGEIDARQAADAAKESGT
                     RKDLMVCGGTGCQASDSKLLVENLNAVIREKGLEKDVHVVTTGCFGFCEKGPIVKVHP
                     DNVFYVEVKPEDAREIVESHIIGGVRVDRLLYEEPTIHKKVPSHSDMSFYKKQMRIAL
                     RNCGFINPENIREYIANQGYIALAKVLEENKPESVIEQIKRSGLRGRGGGGFLTGLKW
                     EIASKHASDEKFMICNADEGDPGAFMDRSIMEGDPHSIIEAMAIAGFAIGASVGCVYI
                     RAEYPLAIHRLEIAINQAREYGLLGNNIFGSGFSFDIELKYGAGAFICGEETSLIHSI
                     EGKRGEPTTKPPFPAESGLWEKPSCVNNVETLANIPEIIRRGAEWYRGIGTETSKGTK
                     VFALAGKINNVGLVEVPMGTTLREIVFDIGGGIPGGKAFKAVQTGGPSGGCIPAQFLD
                     TPIDYESLNRIGSMMGSGGMIVLSEDDCMVNLAKFYLEFSVDESCGKCTPCRIGTKRL
                     HEILTKITEGQGSLKDLERLQELGETIKDTALCGLGQTAPNPVLSTMKYFPEEYRAHV
                     VDRKCPAGACQKLLSYIINEPKCVGCTACARVCPVSCISGSAKKPHVIDQTKCIKCGA
                     CFAKCKFGAIDRK"
     gene            5553..7292
                     /locus_tag="PLD82_02240"
     CDS             5553..7292
                     /locus_tag="PLD82_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG64239.1"
                     /translation="MGLAIVTINDKQIAVEPGTTILEAANKLNIHIPTLCHLDMHDIK
                     MVNKVASCRVCVVEVAGRRNLAPACATPVTDGMVISTNTKRAIKARRAVLELLLSDHP
                     FNCLTCAKSLDCELQALAKQFGIEDVPYKGKMSTYPLDTSSRAIRRELDKCILCRRCE
                     TVCNEMQTVGVLTGYGRGFECVVSPAEMKPLSDTICTFCGQCINVCPTAALTGISYIR
                     EVWDAINNPAKTVIVQTAPAVRVGIGEEFDMPPGRAATGKLVAGLRALGFDKVFDTDF
                     AADLTIMEEAHELIGRLKDGKNLPILTSCCPGWVNFLEFQFPNLINIPSSCKSPQQMF
                     GAIAKSYYAEKIGVAPEDLIVVSIMPCIAKKYEAGRPEFAHESVPDVDYVLTTRELAK
                     MFREAGINPVELNDEAYDDPLGQASGAGVIFGVTGGVIEAALRTAYEWITGKELKDVN
                     FRDVRGMEGIKEATVNIAGTQVNIAVTSGLGNARKILEKIESGEAEYHAIEIMACPGG
                     CINGGGQPFLHGDISRLEARMQAIYDEDERQVVRKSHENPSIKKLYSDFLGEPGSAKA
                     HEILHTHYYKKGE"
     gene            7417..7791
                     /locus_tag="PLD82_02245"
     CDS             7417..7791
                     /locus_tag="PLD82_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64240.1"
                     /translation="MKHAVKILLVVVLMLSATTAFSFELGGSLGYNLLLDDMDGGGLP
                     AIETRITFGDPADSIRFGGMISYLPIYDYSFDSGFGSYSVSSASLCFMGFGKMTIPSS
                     PLYAIAGAGLHMYTGDGSGSDL"
BASE COUNT         1725 a         2588 c         2114 g         1482 t
ORIGIN      
        1 cgatgccggc ctggccatgc accgactggg actcctctcg tcaggtgccg ccgcaaccaa
       61 tgtcccggtc tctcccgtcg tacgcattga tgacgattac gcccgcggca tccagcgagc
      121 cgttctggaa aacaccatcc atacgatcat cctcccctgg tttgaacggg tcccgggaaa
      181 cgattttctc gcggaggatc tctcgtcccg gatccggcaa acagcgtccc atacagatcc
      241 tcctgatcct ccttgacaac agcgaggcgg acacgcacat tgcaaatctt gccctgctgg
      301 cacacatgct ggcggacaag gaactgactc aggccctgat cgacacggac aacccggccg
      361 ccatccggac cctcattgcc agagcactca gccattcccc ggcctgagca tcccgaaaac
      421 aacttgtctc cccgacactg ccgcaggtag actgtggacg tattccggac aaggaggcgc
      481 acgatggccg aggaacaacc ggaccagcag cgaccgctcg agactgtcct cgaggatctc
      541 ggggcagcgg tatttgacat ctccgcaatc gcacggagcc cctcgtccgc cgtcttcagc
      601 ctgcgtcgcc actcgccaac cgctccccgg gaagcgctca agatcctgac catccatgaa
      661 gcctccgctc tcgaaatccc gacgggaaag attcaccccg ggatcctgcc cgtcagcgcg
      721 agcggccaga gcaagacccc ggccggccgg atcttctggt gcctcatgcc ctggctggag
      781 aattcccgca cgctgcgcga ggccctgctt gcccgcctcg aagcaacgga gagcagccag
      841 accacatcca gaatccttgt ctggttgacc gaacggctcg acgaagccgc ggcagccctg
      901 acggccctgc acacacgggg actggcccac ctcgatatca agccggacaa catgctcatc
      961 caggatgacg gaagactggt gatcgcggat accgcctcct gccgcgcact gcgcgggaag
     1021 gaccagtgcg gcatcacgct gacccgctat tttgcccatc ccgatctgcg cactctcctc
     1081 tccgatgccg ccgcgcaaga cagacaggcc ctgatcctgc cccggaacaa actctccttc
     1141 atccatgaca tctacgcctt gggacgatcg atccttgact tgctcaccct gctcgacacc
     1201 cgccacccgg gaagcctgcc ctacgatccc gtcttctcct ggttgcacct ggccgcctgc
     1261 cggatgctcg acggccacaa tctccccgcc tcgggtcctg acgggctctc cgaaatctgg
     1321 cccggccttg accgggagga actggcactc ctccggtaca ggagcatgga ggccgtctcc
     1381 tccgatttcg caaaactgac gggacggagc aggccggaag atgacatccc cgaactggcc
     1441 agtacgggtg gccgccggat catggtatcc cccggccggc ccgctccgct gaccgaccgg
     1501 gtgcgggcta tcatcgagca ccccgttttt tcgaggctgg cctcggttcc ccaaatagag
     1561 cttttgtcct cgatattccc gacagccacc catacacgct acgaacattc cctgggcgcc
     1621 ttccggaacg cggtccgcta tctcgacagc ctgctccatg acatccacac cccgctcttc
     1681 cgccagcttg ttgacaccag gcacctgacg atcctgttgc tcggaacact tgtccatgac
     1741 ctcggccagt atcccttcgg acataccatc gaggaactgg gcgaacattt ccgccacgaa
     1801 accctcagcc gggatttcct cgacaacccg acacgggaca gcagcggccg gaccatccgc
     1861 gagatcatcg aggaccccga gcatggctgg ggaatcccgc tggccgatct caagcgattc
     1921 ctctcgaccc cgtccgggag ggaagacctg tttgatgaac gtcgacccgt cgacgagatg
     1981 ctgcattcca tcctcgatgg tccgatcgat gtcgacaagc tggactatct ggtccgtgac
     2041 agccggatgt gcttcctcaa ctacggcgat gctatcgaca gcgaacgcct gatcaccaac
     2101 ctgacgatcg tcatccagca cgaacaggac gccaatggcc gtgaaacccg ccgcctctgt
     2161 ctcggggcct acgaacgggg cgagccggcg gccgaagccc tctcctttgt ccgctacctc
     2221 ctctaccaga ccctctactg gcaccatgcc tcgagggcgg gacggacgat gttcaaggcg
     2281 gccctggccc cccttgtcac ggcatccggc aagcgccctg cccggggatc acgctaccac
     2341 cccttcatgg cagacattgc acgcctgctc ggcgtagccg aagaacctgg cccgatcaca
     2401 ctctccgatg tcctggacca ttttgagaaa aaggccgacg agcctggccg agaactgatc
     2461 gcactgatcc gtgccagacg ctattacaaa cggatctgca ccatccatga gggcagcgga
     2521 agccggatac agatcgagga tttccgcaac gccacccgga aaccgggctt tgacgagacc
     2581 ctgcggaaac tccttcgcgc ccgtttcgaa agccggctga cctcacgcca gggacctggg
     2641 gcctccctcc tcactcccga ccggagcgga ccggccctcg aaattctctc cagacaacgc
     2701 accatcctct gcgatgcccc acggcccagc atcgggtcgc acagcaagct ccactttgtt
     2761 cccgagccgc agcgtcttga gcggaatcat tccgaacggc acctctcggg cgagcgggtc
     2821 tcggccgtct ggaacgatgt ccatgccaga ctgatgtcga tcgccgccaa gggccgggtc
     2881 ttttgtcacc ctgacctcag ggaggcgctt ctggccgccc tcggcccggc tggtgtcgag
     2941 gactgcatcc ttgaggccat gaaaaaatga gcctgccatt acggccggcc ggattttgac
     3001 aaacaattaa caatctcgct tgaccatact cttctccacc aagtattttt ctggagaagt
     3061 acgtacccag tcaaggagac cgaccatgag tccgaccgtc aagctcggcc cgcgcaccct
     3121 gccggccgag tcctatgcca agctcgacac atacatcgac agcctcggca tcaccaaaca
     3181 ggatccccgc aagaagggct tcctcatcca gatcctgcac aaggcccagc acatttttgg
     3241 ccacctaccc gaggaagtgc agaaacacgt cgccaaccgg ctctggctgc accatgcaga
     3301 cgtctccgga gtgatcagtt tctacaatta tttcaccaca atccccaagg ggaagatcag
     3361 catcagcatc tgcatgggca ccgcctgtta cgtcaaggga gccgacaaga ttctggccga
     3421 attcgaaaaa cagctcggca tccaggccgg cggcctgacc gatgacggtc tcttctctct
     3481 ggatgttctg cgctgcgtag gcgcctgcgg cctggcccct gtcgtcctgg tcaacgagaa
     3541 ggtctatggc cggctcaagc ccgaggatgt ttcccgggtg atcactgaag cccgtaccat
     3601 gcataccgct ccggaggctg caccatgacc cgcattgact cctttgaagc cctgcgcacc
     3661 atggtgcgtg aatccgccag cctccttgat ctccgccacc acgccgggga gatcgatgcc
     3721 agacaggctg ccgatgccgc gaaagaatcc ggaacccgca aggatctgat ggtctgcggt
     3781 ggaaccgggt gtcaggcatc cgacagcaag ctccttgtcg agaacctcaa cgccgtcatc
     3841 cgcgagaaag gtctcgaaaa agacgtccac gtcgttacaa cgggctgttt cggcttttgc
     3901 gaaaagggtc cgatcgtcaa ggtccatccc gacaacgtct tttacgtcga ggtcaaaccg
     3961 gaagacgccc gcgagatcgt cgagtcccac atcatcgggg gagtccgggt tgaccggctc
     4021 ctgtacgaag aaccgaccat ccacaagaag gtcccgtccc acagtgacat gtccttctac
     4081 aagaagcaga tgcggatcgc cctccggaac tgcggtttca tcaatcctga aaacattcgc
     4141 gaatacatcg ccaaccaggg ctacatcgcc ctggcaaaag tccttgagga aaacaagccg
     4201 gaatccgtca tcgaacagat caagcgctcg gggttgcgcg ggcggggagg cggcggattc
     4261 ctgaccggac tcaagtggga aatcgccagc aaacatgcct ccgacgagaa gttcatgatc
     4321 tgcaatgcgg acgagggcga cccgggcgcc ttcatggacc gctcgatcat ggaaggcgac
     4381 ccgcacagca tcatcgaggc catggcgatt gccgggtttg cgatcggggc ttccgtcggg
     4441 tgtgtctaca tccgggccga gtacccgctg gccatccacc gtctggagat cgccatcaac
     4501 caagcacggg aatacggact cctgggaaac aatatcttcg gaagcggttt ctcgttcgat
     4561 atcgaactca agtacggtgc tggcgccttc atctgcggcg aagagacctc cctgatccat
     4621 tccatcgagg gcaagcgcgg cgaaccaacc accaaaccgc ccttcccggc cgaaagcggc
     4681 ctctgggaaa aaccgagttg cgtgaacaat gtcgagaccc tggcaaacat tccggaaatc
     4741 atccgccggg gcgccgaatg gtaccgtggc atcgggacgg aaacttccaa gggaaccaag
     4801 gtttttgccc tggcgggaaa aatcaacaat gtcgggcttg tcgaagtccc catgggaacg
     4861 acgctccggg aaatcgtctt cgacatcggt ggcggtatcc cgggcgggaa ggcattcaag
     4921 gcggtccaga ccggaggccc ctccggcggt tgcatcccgg cccagttcct cgacaccccg
     4981 attgactacg aatccctcaa caggatcggc tccatgatgg gttctggcgg aatgatcgtt
     5041 ctctccgagg acgactgcat ggtcaatctg gcaaaatttt atctcgaatt ttccgttgac
     5101 gagtcctgcg ggaaatgcac cccctgccgg atcgggacca aacgcctgca cgaaatcctg
     5161 acaaaaatca ccgaaggtca gggatccctc aaggatctcg aacgcctgca ggagctgggc
     5221 gagacgatca aggataccgc actctgcggt ctcggccaga cggcacccaa tccggtcctc
     5281 tccacgatga aatattttcc cgaggaatac cgggctcatg tcgttgacag aaagtgtccc
     5341 gccggcgcct gccagaaact cctgtcctat atcatcaacg aacccaagtg tgtgggctgt
     5401 accgcctgcg cccgtgtctg tccggtcagc tgcatttccg gctcggcaaa gaaaccgcac
     5461 gtcatcgacc agacaaaatg catcaagtgc ggcgcttgct tcgcgaaatg caaattcggc
     5521 gccatcgaca gaaagtaaga ggatactcga ccatgggtct tgccatcgta accatcaatg
     5581 acaaacaaat cgccgtcgaa ccgggaacaa ccatcctcga agcggccaac aagctcaata
     5641 tccacatccc gacactctgc caccttgaca tgcacgacat caaaatggtc aacaaggtgg
     5701 catcctgccg cgtctgtgtt gtcgaagtcg ccgggagacg gaatctggct cccgcctgcg
     5761 ccaccccggt gaccgacgga atggtcattt ccacaaatac caagcgcgcg atcaaggccc
     5821 gccgggccgt cctcgagctg ctgctctccg accacccctt caactgtctg acctgcgcca
     5881 aatccctgga ctgcgagctc caggcgctgg ccaagcaatt cggaatcgaa gatgtcccct
     5941 acaagggaaa gatgtccacc tacccgctgg acacctcaag ccgggccatc cgcagggaac
     6001 tcgacaaatg cattctctgc cgacgctgcg aaacagtctg taacgagatg cagaccgtgg
     6061 gagttttgac cgggtacggt cgcggctttg agtgcgtggt cagcccggcc gagatgaagc
     6121 cgctttcgga taccatctgc accttctgcg gccagtgcat caatgtctgc ccgaccgcgg
     6181 ccctgacagg catcagttat atccgcgaag tctgggatgc aatcaacaat ccggccaaaa
     6241 cggtcatcgt ccagacagcc ccggccgtgc gcgtgggcat cggcgaggaa tttgacatgc
     6301 cgccgggacg ggccgcgacc ggaaagctcg tagccggcct gcgagccctc ggttttgaca
     6361 aggtctttga caccgacttc gccgccgacc tgaccatcat ggaagaggcg cacgaactga
     6421 tcgggcggct caaggacggc aagaatctcc cgatcctgac atcctgctgc ccgggctggg
     6481 tcaactttct cgagttccag ttccccaacc tgatcaatat cccttcttca tgcaaatcac
     6541 cacagcagat gttcggagca atcgccaaga gttattacgc cgaaaagatc ggggttgcac
     6601 ccgaagacct gatcgtcgtc tcgatcatgc cctgtatcgc caagaaatac gaggcaggcc
     6661 gccccgagtt tgcccacgaa agcgtccccg atgtggacta cgtcctcacc acacgcgaac
     6721 tggccaagat gttccgcgaa gccgggatca atccggtcga actgaacgac gaagcctacg
     6781 acgacccgct cgggcaggcc tcgggtgccg gcgtaatctt cggtgtcacc ggcggtgtga
     6841 tcgaggccgc cctgcgtact gcctacgaat ggatcaccgg caaggagctt aaagacgtca
     6901 acttccgtga tgtccgcgga atggaaggta tcaaggaagc aaccgtcaat atcgccggga
     6961 cccaggtcaa catcgcggtg acctccggtc tggggaacgc tcgcaagatc ctcgaaaaaa
     7021 tcgagtcagg cgaggccgag taccatgcca tcgagatcat ggcctgcccc ggtggttgca
     7081 tcaatggtgg aggccagccc ttcctccatg gtgatatctc gcgtcttgag gcccgaatgc
     7141 aggccatcta tgacgaggac gaacgccagg tggtgcgcaa atcccatgaa aacccgtcca
     7201 tcaaaaaact ctactcggat tttctgggcg aaccggggag tgcaaaggca cacgaaatcc
     7261 tgcatacgca ttattacaaa aagggtgaat gaacagaccc tgacaaacag agggaccgga
     7321 ggagggacac tactccggtc tttgttttta tgcagtcttg cagtatactt ctcattcgat
     7381 gccggctttt cttgcaaaac actcaggagg tcttttgtga aacatgctgt gaaaattctt
     7441 ctcgtcgtag tcctcatgct ttctgccaca accgctttca gcttcgaact cggcggatcc
     7501 ctcggctata acctgcttct ggatgacatg gatggcggtg gactgccagc catcgagacc
     7561 cgcatcacgt tcggcgatcc agccgattca atccgctttg gtggtatgat cagctatctg
     7621 ccgatatacg actattcttt cgattcaggg tttggttcgt actctgtcag ctcggcatcc
     7681 ctctgtttca tgggcttcgg aaaaatgacc atcccgagct cacccctgta cgctattgca
     7741 ggcgccggac tccacatgta cacgggtgac ggatccggaa gcgatctctg agtcatgatc
     7801 ggtgccggtt tcgaaacacc catcgccgaa aacatgacgc tggatatcgg cgcccgcttt
     7861 cacatgatcc ttgccagcag ttctgtcaac atgttgacaa tccttggcg
//
LOCUS       DAOSHE010000044         7836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8022_length_7836_cov_3.972240, whole genome shotgun sequence.
ACCESSION   DAOSHE010000044 DAOSHE010000000
VERSION     DAOSHE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_7836_cov_3.972240"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLD82_02250"
     CDS             complement(<1..631)
                     /locus_tag="PLD82_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG64241.1"
                     /translation="MKQTFTLLSLASLLFLATCGSAKITWHETLESAQAEAAKTKTTI
                     LVNFTGSDWCTWCQKLATEVFDAPDFASDAGQHFTFVKLDYPRTIEQPEATKQYNEKL
                     MTQFGIEGFPTILLLDASGKAYARTGYQEGGAKAYLTHLLSLSNQRVLRDTLLKTTIR
                     ETDPAKKIDALVSAISNLSAWGILASYPELKGEVIRLDADDARGLRSKYS"
     gene            778..1254
                     /locus_tag="PLD82_02255"
     CDS             778..1254
                     /locus_tag="PLD82_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64242.1"
                     /translation="MKKFFMAGVLLVLVGVAGCSKSAGGDDANAYISEMIPLLIVKTG
                     LDNAFSERFEKAVNGQAVVSIYSQYMATNQSLGQLVTGIQDKYKGVTREAFMAAYSNR
                     KAEMSDVEMKNEMATMRLQVAFLSNQHRDSVEVKKLFSDLSNQAYAQMQQQPPTVQ"
     gene            complement(1336..1872)
                     /locus_tag="PLD82_02260"
     CDS             complement(1336..1872)
                     /locus_tag="PLD82_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG64243.1"
                     /translation="MIRFLVMTLLVIALLPGCFVTVSERSQNDRGTSGANTVTDEPSS
                     TGGVSDSVRTVERRAFDLINQYRRSIGLPSLIWNEAIARQCRNHSQYMANRGSLSHDN
                     FSQRLNNLRSSMQVMAGAENVAMNYNMPDPALVAKNGWIRSPGHHKNIKGNYTHSGMG
                     VVRTSDGKYYLTQVFVRQ"
     gene            2158..4242
                     /gene="fliD"
                     /locus_tag="PLD82_02265"
     CDS             2158..4242
                     /gene="fliD"
                     /locus_tag="PLD82_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOG64244.1"
                     /translation="MAIPMPGLAVQGLDINKTIEKLVTVKREPLKRMESEIEELNLQK
                     DIWAALRQRLVTLGNLAKRMYDYTNPFGVLIAESGDKESFTATAERKAKKGQRRVQVM
                     QIASAHRIASDSIKKEQKLPGGIFYIEMGSKKVRVDFSSGGTLADLSSRIEVAGREVV
                     RPSLTHDTTETSILVLDAVKGGTGNVLRFSGDVRILKEAGLLTEGRAEEMRVPLDGPS
                     SWTTLSPAGAVWSVRDGMAALAPGARIEYGLPRAVTAAAGWTFGFSLMFETNSRPRTN
                     QTAVGQTVRTNILIGPDDPLKIEDLTIFGPRLVAGIPVDKTNRAVTNAPARAAGTNSS
                     AVVRLVEGGDVRTETAVPAAFRHGVWQDISLPLTQLANVQAPARVLLVNENTAARVTV
                     KDMVFRLSKDGGLVAKNELVAPRDAIVSIDGVRITRPDNAISDAIEGVALRLIKPGKE
                     TMLDIRHDVDLVSKYILELVEGYNQTLLYMNAVTKNLTREERQKLESDQKNKTDLMKA
                     LEDKDKADEEKHKGRLPGDMTISQLKSSLGVIMMSPYKTRLERELTLLMQIGISTGKI
                     GATWADLKNSAGMLELDTSVLKSMIEKDILAVAQIFGNDLNGDFVVDHGACFRLYELT
                     RIWTQPGNSGVIAVKVRSLDRVIVEKRKSIDRVEMSIKAYEDRLRAQFGSMETRVREY
                     QERGRWLNRQGQ"
     gene            4264..5463
                     /locus_tag="PLD82_02270"
     CDS             4264..5463
                     /locus_tag="PLD82_02270"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG64245.1"
                     /translation="MQDEHLVRLPDGRVRIDVRFLVPGTVLAWPTFNDANEMVHLAYR
                     PFTADEVEDLIREGVEYLYYTGQTDVGRQYVKDLKGYLDQEIYQGPRTITVETQKKAV
                     SAMMEIVQFIKSGQVTDFSDAKNIVEYVLNDIHTSKDDFINLLDIQEYDDYTYTHSLN
                     VGVIAMVLGKRMNLSDAVLFNVGMAGFLHDIGKLRIPNEIINKPRKLTDEEYRTIKNH
                     PRFGYEMVKGSPHVNEVVQRLILFHHERADGSGYPLGLTQEKLGNLTFIIAIADFYDA
                     MTSARSYKRALTPKEAMQVIMKQAVTHFPDKVVQQFMADMKDILREGSFYELGMIVLL
                     NTREVGRVVDKDFTATTRPVLEILRNSRGEVLKKPIRVDMNYDGSRYISKILSNDDSA
                     IVDEYFG"
     gene            5529..5843
                     /gene="rplU"
                     /locus_tag="PLD82_02275"
     CDS             5529..5843
                     /gene="rplU"
                     /locus_tag="PLD82_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG64246.1"
                     /translation="MYAVAEIAGKQYVIEEGKTVAVDRLDAEDGASIDIDKVCLVRSD
                     DGQVKVGKPYVGGAVIKAKVERNFRARKILVFFFRQKKDSRKKRGHRQEHSLLKIEKI
                     MV"
     gene            5843..6214
                     /locus_tag="PLD82_02280"
     CDS             5843..6214
                     /locus_tag="PLD82_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOG64247.1"
                     /translation="MLRFVSLYDEAGRPVGFEASGHAGDAAKGGNLACAAASALIQTL
                     VDGLSGEMGLTLSLTTGKDGFLSCLVQGVPDQAVQDKMELLLRVVGRGLESIAEMDRT
                     KSTVVLRERRREVTRSMENGT"
     gene            6204..6467
                     /gene="rpmA"
                     /locus_tag="PLD82_02285"
     CDS             6204..6467
                     /gene="rpmA"
                     /locus_tag="PLD82_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG64248.1"
                     /translation="MAHKKGQGSAKNGRDSIGKRLGVKRFAGEVVTGGSILCRQRGTK
                     FYPGKNVGKGSDDTLFAKISGRVAFETYHGTKRQISVYPEEAK"
     gene            6731..>7836
                     /gene="prfA"
                     /locus_tag="PLD82_02290"
     CDS             6731..>7836
                     /gene="prfA"
                     /locus_tag="PLD82_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG64249.1"
                     /translation="MIDRLEEIDRRAREVAETLSRPETVADSRRFRELAREHARLEQG
                     SRLLAAYRGTTQRLAQAEEMLLSEKDAELVAMAEDEARSARDELAGLEQEIRLFLIPP
                     DSRSGRDIIVELRAGTGGNESALFVADLVRMYTRFAEDAGLKVELLSSHPTELGGYKE
                     FIIAVSGERAWDLFRFESGAHRVQRVPETESGGRTHTSAATVAVMPEAEESEIVIGDK
                     DLRIDVYRSSGPGGQSVNTTDSAVRITHIPTGLIVTCQDEKSQHKNKAKALKVLRARL
                     AERMEAERNSELSAMRRSQIGTGDRSERIRTYNFNENRVTDHRIHETWHKLPEIVAGN
                     LNEVVAALEKDRRETLLAEIARSGFRQRSPGVCA"
BASE COUNT         1742 a         2090 c         2436 g         1568 t
ORIGIN      
        1 cgctgtactt cgagcgcaga ccgcgggcat catcggcgtc caggcggatg acctcccctt
       61 ttagctcagg gtagctggcg aggatccccc aggcggagag attgctgatc gccgagacaa
      121 gggcatcgat cttttttgcc ggatccgtct cccgaatggt tgtcttgaga agggtgtcgc
      181 gcaggaccct ctggttgctc agggagagca ggtgggtgag ataggctttg gctcccccct
      241 cctggtagcc tgtcctggca taggccttgc cgctcgcgtc caacagcagg atggtcggaa
      301 atccctcaat accgaattgg gtcatgagtt tctcgttgta ctgcttggtt gcttcgggtt
      361 gttcgatagt ccggggataa tccagtttga caaaggtgaa gtgttggccg gcatcggatg
      421 caaaatccgg ggcgtcaaag acctcggtag cgagtttttg gcaccaagtg caccagtccg
      481 agccggtgaa attgacaagg atcgtcgttt tggttttggc ggcctctgcc tgggcggatt
      541 cgagggtctc gtgccaggtg atctttgctg aaccacaggt cgcgaggaag agaagagacg
      601 ccagagaaag aagagtgaat gtttgtttca ttgaaaaccc catatatatg gattttataa
      661 aatctaggct gggagagaca ggaatgcaag acaaatccgg cttgttcaaa aatatgccga
      721 ttcccgtcct gttggctagc tttattgctt cggaggaaag aactccgtgg aggaagaatg
      781 aaaaagtttt ttatggccgg ggtgctcttg gtccttgttg gcgtggccgg gtgcagcaag
      841 tcagccggtg gggatgacgc caacgcctac atcagcgaga tgatccccct gcttatcgtc
      901 aagaccggtt tggacaatgc gttttccgag aggtttgaaa aagctgtcaa cgggcaggcg
      961 gtcgtctcga tctactcgca gtacatggcg accaatcaga gtcttggcca gctcgtgacg
     1021 ggcatccagg acaagtacaa aggcgtgacc cgggaggcct tcatggcggc ctacagcaac
     1081 cgcaaagccg aaatgagtga tgtcgagatg aaaaacgaga tggcgaccat gaggctccag
     1141 gttgccttcc tcagcaacca gcaccgggac tccgtcgagg tcaaaaagct gttcagcgat
     1201 ctcagcaatc aggcctatgc ccagatgcag cagcagcccc cgacggtcca gtaaccgttt
     1261 ttttgctggt ttgtcacaaa aaagaggctc tcctgaaact gtcgggagag cctccttgca
     1321 ttccgggtcg tccggtcatt gccgtacgaa gacctgggtc aggtagtact ttccgtccga
     1381 ggttcggacg acgcccatcc ccgagtgagt ataattgccc ttgatattct tgtggtgtcc
     1441 cggactcctg atccagccat ttttggcgac cagggctggg tcgggcatat tgtagttcat
     1501 ggcgacgttt tcggccccgg ccatgacctg catcgaactc cggagattgt tgaggcgctg
     1561 gctgaaattg tcgtgggaga gacttccccg gttggccatg tactggctgt ggttgcggca
     1621 ctggcgggcg atggcctcgt tccagatcag ggacgggaga ccgatcgagc gccggtactg
     1681 gttgatgagg tcaaaggcgc gacgttccac cgtccggacc gagtcggaaa caccgccggt
     1741 cgagcttggc tcgtctgtaa cagtgttggc cccggatgtg cccctgtcat tctgtgatct
     1801 ttcggaaacc gtgacgaagc aaccgggcag cagggcgatg acaaggagtg tcatcacaag
     1861 aaaacggatc atgtcgtcct ccaaatttgt gtgtatggta gagtgtacac cccggttggc
     1921 gtcgtatcac ttcttttttg gggaacagtc tgccgcccga caggctcgag gttgttcatc
     1981 aaaaatcttc ttgcaccggg caggccaagc ttcgtcgttc gttcccggca atgtccgcag
     2041 ggctgcggaa gccgtgaaaa aaaccgggga ggagagaaaa aaggactcaa gcttttgtct
     2101 ggatgaccga ttcttgacgt gagggagtcg cagcgcgcga ctgcaagggg ggaactcatg
     2161 gccattccca tgccggggct tgccgttcag ggcctcgata tcaacaaaac catcgaaaaa
     2221 ctggtcacgg tcaagcgtga accgctcaag cggatggaga gcgagattga agaactcaac
     2281 ctccaaaagg atatctgggc cgccctgcgc cagcgactgg tgacactggg caatcttgcc
     2341 aaacggatgt acgactacac gaatcccttt ggcgtcctga tcgccgaatc cggtgacaag
     2401 gagtccttta ccgccacggc ggaacgcaag gccaaaaagg gacagcgcag ggttcaggtc
     2461 atgcagatcg ccagtgcgca ccgtatcgcg tcggattcca tcaaaaaaga gcagaagctc
     2521 ccgggcggaa tcttttacat cgagatgggg agcaaaaagg tccgggtgga tttctccagt
     2581 ggcgggacac tcgccgatct ttcgtcacgg atcgaggtgg cgggccgcga ggtggtgcgc
     2641 ccctcattga cacacgatac gaccgagact tccattcttg tgctggacgc cgtcaaaggc
     2701 gggaccggga atgtcctgcg gttttccgga gatgtccgga tactcaagga ggccggactc
     2761 ctgaccgagg gccgggccga ggagatgcgt gttccccttg atggaccgtc ttcatggacc
     2821 acgctctctc ctgccggtgc ggtctggagc gtccgggacg gaatggcggc cctggctccc
     2881 ggggcgcgga tcgagtatgg tctgccgcgg gctgtcacgg ccgctgccgg ctggaccttc
     2941 ggcttttccc tgatgttcga aaccaattcc cgcccgcgga ccaaccagac ggcggtgggc
     3001 cagacagtcc ggaccaatat cctgatcggg ccggatgacc cgttgaagat cgaggatctg
     3061 acgattttcg ggcctcgcct ggtggcaggg attcccgtgg acaagacaaa ccgtgcggta
     3121 accaatgccc cggcccgggc agcgggcacc aacagctcgg ccgttgtccg tctcgtcgaa
     3181 ggcggggatg tgcgcaccga aaccgccgta ccggcagcct tccgccacgg tgtctggcag
     3241 gacatttccc ttccgttgac gcagttggcc aatgtccagg ctccggctag ggtccttctg
     3301 gtcaacgaga atacggcagc ccgtgtgacg gtcaaggata tggtgttccg tctctccaag
     3361 gatggcgggc tggtggccaa aaacgaactc gtggctcccc gggatgccat tgtgagtatc
     3421 gacggggtgc gcatcacccg tccggacaac gcgatcagcg atgccatcga aggtgtggcc
     3481 ctcagactga tcaagccggg caaggaaacg atgctggata tccggcatga cgtggatctc
     3541 gtctcgaagt acattctcga gctggttgag gggtacaacc agacgcttct ctatatgaac
     3601 gccgtcacaa aaaacctcac gcgcgaggag aggcaaaagc tcgagagcga ccagaaaaac
     3661 aagaccgatc tgatgaaggc cctcgaggac aaggacaagg ccgacgagga gaagcataaa
     3721 gggcgccttc cgggtgatat gacgatctcc caactgaaga gttccctcgg ggtgatcatg
     3781 atgagcccct acaaaaccag actcgagcgg gagttgaccc tcctgatgca gatcggcatt
     3841 tcgacgggca agatcggcgc gacctgggcc gatctgaaaa attcggccgg catgctcgaa
     3901 cttgatactt ctgtcttgaa aagcatgatc gaaaaggata tactggctgt tgcacagatt
     3961 tttggaaacg atctcaatgg cgattttgtt gtcgatcacg gggcttgttt ccggctgtac
     4021 gaattgacac ggatctggac ccagccgggc aactcgggtg tgattgccgt caaggtccgg
     4081 agtcttgacc gggtaattgt cgaaaaacgc aagtcgatcg accgggtcga gatgtcgatc
     4141 aaggcctacg aggaccgcct gcgtgcccag tttggcagca tggagaccag ggtgcgcgag
     4201 tatcaggagc gggggcgctg gttgaaccgg cagggacagt aggcagggag ataaggggag
     4261 cgtatgcagg acgaacatct tgtccggttg ccggatggcc gcgtgcggat cgacgtgcgt
     4321 tttcttgttc ccggaacggt tctcgcctgg cccaccttca atgatgccaa cgagatggtg
     4381 cacctcgcgt atcgtccgtt caccgccgat gaggtcgagg acctgatccg ggaaggtgtg
     4441 gagtatctct actacacggg acagaccgat gtcggtcggc agtacgtcaa ggatctcaag
     4501 ggctatctcg atcaggagat ctatcagggt ccccggacca tcaccgttga aacccagaaa
     4561 aaagccgttt ccgccatgat ggagatcgtg cagttcatca agagcggaca ggtgacggac
     4621 ttctcggatg ccaaaaacat tgtcgagtac gtactcaatg acatccatac atccaaggac
     4681 gacttcatca acctcctcga tatccaggag tatgatgact acacctatac gcattcactg
     4741 aatgtgggcg tcatcgccat ggtactgggc aagcggatga atctctcgga cgccgtcctc
     4801 ttcaacgtgg ggatggcggg ttttttgcac gacatcggca agctgcggat tcccaacgag
     4861 atcatcaaca agccgcgaaa gctgaccgac gaggagtacc ggacgatcaa gaaccacccg
     4921 cgcttcggat acgagatggt caagggttcg ccccatgtca acgaggtcgt ccagcgcctg
     4981 atcctcttcc atcatgagcg ggcggatgga tcgggctatc ccctcgggtt gacccaggaa
     5041 aaactcggga atctgacctt catcatcgcg atcgccgatt tttacgatgc gatgacctcc
     5101 gcccgctcct acaaacgggc cttgacaccc aaggaggcga tgcaggtcat catgaagcag
     5161 gccgtgacgc atttccccga caaggtggtc cagcagttca tggcggacat gaaggacatc
     5221 ctcagggagg gctcattcta cgagctgggg atgattgtgc tcctcaatac acgggaagtg
     5281 gggcgggttg tggacaagga tttcacggcg acgaccagac cagtgctcga aattctgcgc
     5341 aattcgcgcg gtgaggtctt gaaaaagccg atccgcgtgg acatgaacta cgacggaagc
     5401 cggtatatca gcaagatcct cagcaatgac gattccgcca tcgttgacga gtattttggg
     5461 taaaatcccc ttgaccgccg gcccggcggt tcatactttt gacagacatt gagctggaga
     5521 caaaaagcat gtacgcagta gccgaaattg caggaaaaca gtacgtcatc gaagaaggca
     5581 agacggtggc ggtagaccgc ctcgacgccg aggacggcgc gagcatcgat attgacaagg
     5641 tttgccttgt gcgcagcgat gatggtcaag tcaaggttgg caagccctat gtgggtggcg
     5701 ccgtgatcaa ggccaaggtc gagcggaact tcagggctcg caagattctg gtgtttttct
     5761 tccgccagaa gaaggactcc cggaagaagc gtggtcacag gcaggaacac agcctgctta
     5821 agatcgagaa gatcatggtg tgatgcttcg cttcgtcagt ctgtatgacg aagcggggag
     5881 gcccgtcggt tttgaggcct ccggacacgc cggggatgca gccaagggcg gaaatcttgc
     5941 ctgcgctgcc gcctcggctc tgattcagac cttggtcgac ggcctgtccg gcgagatggg
     6001 tctcacgctt tccctgacaa cgggaaagga cgggttcctc tcctgcctgg tgcagggtgt
     6061 cccggatcag gctgtacagg acaagatgga attattgctc cgtgtcgtcg gtcgtggact
     6121 tgagtcgatt gcggagatgg acaggacaaa gagtaccgtt gttttgagag agagacggcg
     6181 ggaagtgaca aggagcatgg agaatggcac ataaaaaggg tcaaggtagc gcgaaaaacg
     6241 gtcgtgattc catcgggaag cgtctcggtg tcaagagatt cgccggtgag gttgtcacgg
     6301 gtggatccat tctctgccga cagcgcggga cgaaattcta tcccgggaaa aatgttggca
     6361 agggcagcga tgataccctc tttgccaaaa tctcgggtcg ggttgcgttt gagacctacc
     6421 acggaacaaa gcgccagatc agcgtctatc ccgaagaggc gaaataatcc ggaagcggtt
     6481 ggcctgagca gggtcacgtc agaaaatctg gcggagatga tgatcctgaa ggggtgccaa
     6541 tccgatgcag agacgctgtt tatgcgttgg accgggatgg tggattcttc catcccgtcg
     6601 cttcaggaaa cccggttgct ttggtgccgg tgtgattcgg atacgaacca gacgagccct
     6661 ccacctcatg tagcggggag ggctttgtcg tacagggaat ccaggatgtg tgaggaggac
     6721 gggaatggcc atcattgacc ggcttgaaga gatagaccgg cgggcccgcg aggtcgccga
     6781 gacactctcc cggcccgaga cggtggccga ttcccgccgc ttccgcgaac tggcccgcga
     6841 acatgcccgc ctcgaacagg gaagccgtct cttggcggcg tatcggggga ccacgcagcg
     6901 tctggcgcag gccgaggaga tgctcctttc cgaaaaggat gccgaactcg tcgcgatggc
     6961 cgaagatgaa gcccgatccg cgcgggatga actggcagga ctcgagcagg agatcaggct
     7021 cttcctcatc ccgccggatt cccggtcggg gcgcgacatc atcgtggaac tccgggccgg
     7081 aaccggcgga aacgagtccg ccctgtttgt ggccgacctc gtgcggatgt atacccggtt
     7141 tgcagaggac gctggactca aggtcgaact tctttcatcc catccgacag aactcggcgg
     7201 gtataaggag ttcattattg ctgtctcggg cgagcgggcc tgggacctct ttcgcttcga
     7261 atcgggggcg caccgtgtcc agcgggtccc cgagaccgag agtggcgggc ggacccatac
     7321 ctcggcggcg acggtggcgg tcatgcccga ggccgaggag agcgagatcg tgatcggtga
     7381 caaagatctt cgaattgatg tctatcgctc ctcaggcccg ggtggacaga gcgtgaacac
     7441 gacggacagt gccgtacgga ttacccacat cccgaccggc ctgattgtca cctgccagga
     7501 cgaaaagagc caacacaaaa acaaggccaa agccctcaag gtcctccggg cccggctggc
     7561 cgaacggatg gaggccgagc gcaacagcga gctctcggcg atgcgccgga gccagatcgg
     7621 aacgggtgac cgttcggagc ggatccggac ctacaacttc aacgaaaacc gggtgaccga
     7681 ccaccggatc catgaaacct ggcacaaact cccggagatc gttgccggaa acctgaacga
     7741 ggtcgtggcc gcactcgaaa aagacaggcg ggagaccctt ttagccgaga ttgcccgatc
     7801 aggtttccgg caacgatctc cgggagtttg tgccag
//
LOCUS       DAOSHE010000045         7744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8142_length_7744_cov_3.302120, whole genome shotgun sequence.
ACCESSION   DAOSHE010000045 DAOSHE010000000
VERSION     DAOSHE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_7744_cov_3.302120"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1371)
                     /gene="murA"
                     /locus_tag="PLD82_02295"
     CDS             complement(103..1371)
                     /gene="murA"
                     /locus_tag="PLD82_02295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG64250.1"
                     /translation="MDAFSIEGPVRIAGDTTAAGSKNSSLPILAGCLLTDDECIIENV
                     PDLRDVRTMLRLLEMLGRKVERRGGDVIVHAGRVTSTEAPYELVSTMRASISVLGPLL
                     AKEGEARVSFPGGCAIGARPIDLHLKGLEALGAEIEITHGYVHACSPKLKGATMYLAG
                     SAGSSVLATDNVMMAAALADGVTVIQSAAIEPEVVDACHFLVAMGAKIEGIGTTTLTI
                     TGVPRLHGCRYRIIPDRIEARTLLAAAACTGGDVLVRGADPAHLGIVLETFRRAGMDI
                     VADQAGIRLRAGSKIHSVDFETLPYPGYPTDMQAQMMAVLAHAGGTSVITEKIYPERF
                     IHVAELNRMGADIRLEGQTAVVRGVPKLEGAPVMASDLRAGAALVVAALAAEGTSLVR
                     RIYHVDRGYEKLEEKLMALGARITRVKDQS"
     gene            1710..2033
                     /gene="trxA"
                     /locus_tag="PLD82_02300"
     CDS             1710..2033
                     /gene="trxA"
                     /locus_tag="PLD82_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG64251.1"
                     /translation="MSNATPITDATFQSEVINSSIPVLVDFWAPWCGPCRAIGPVIDE
                     IAAERAGKLKVCKMNVDEEQETAGQFQVMSIPTLILFKDGNVAAKIVGAQAKDKLLSQ
                     IDPLL"
     gene            2109..3509
                     /locus_tag="PLD82_02305"
     CDS             2109..3509
                     /locus_tag="PLD82_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG64252.1"
                     /translation="MSRLVLLVSVLVTLATSVQAATNAQAGSSQSQATTSTQASSSQS
                     QTATSTQATNQSGVVSSATNRPPQDLKTVPKKEDLEYWQETMLYGTEQQKDSVLKTMQ
                     SVQAKQVDDILLAALANESEGPIQLKLVQILYDRRITGALGPLVRTLTNAREADTIGP
                     AIAALARFKDPGVLPYILQHLTNETLSVQQAAVRALGEIGDPAPGKRLLEMLDSLPAA
                     SELRYDLVNALGQMKYGPAYESLRTIAINSGNPRYLRAFAITALGSIGNRKIVPDFIK
                     LLSEEKIPDIKVRIIAALGEMPGSESVQAIRIAMNDGDERIRASAIQAAAKSKDEAYL
                     RPLLYKFRYDNEARVMLAAAEALVELKYPELPALILAKFESTRDVHVMSRFVTILKKL
                     PPQKQAIPMLRRKQKENSFAKVKDEIEALLTLWGEKAASDKSPKLDETKKAATPEKKA
                     EDTKSGQWGDRIYLKE"
     gene            complement(3514..4878)
                     /locus_tag="PLD82_02310"
     CDS             complement(3514..4878)
                     /locus_tag="PLD82_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64253.1"
                     /translation="MKPISEASARGRTRAVFFAGALVVLGAVACVLWLLLRQTDFLEV
                     ESVEGGVRWVGRNPETGQAFDLRPEPGKTHDGDVRVLLEPGARLVLSVLERGRLVVEG
                     PSVTTATQSVPSADIRLFKLREDWWRLDEGRAWLVPASREDGDQCHDLRFSLDVDTTN
                     LRAGGFLRLALRLGSTNTVSRVGGMLADASLILVPRPGGREYSALAGLDCMPPAGILR
                     LAVWVHGRDGAWYMRAWQMPVEKFREDREGLRFLARQARPFPGSAYPPAPADWWQRWL
                     QKKESILSGGKPEREYWRLQKIYRASGEQRGEWPVKLRIPIARYDRVSSPFGVVRYLT
                     STNGKAHRGIDFAFPEGVDVVAPMEGRVAFAHETAVHGGLVILDHGDGVFSTFMHLSR
                     VIASSNARVGTGSPVGLVGTTGLSTGPHLHYELRIGNRCVDPTPWFVKSPLAGQPDWI
                     RLPD"
     gene            complement(4886..6103)
                     /locus_tag="PLD82_02315"
     CDS             complement(4886..6103)
                     /locus_tag="PLD82_02315"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOG64254.1"
                     /translation="MPVFSSEFIRAIPKTDLHVHLDGSLRLTTLIELAKEQNVSLPAY
                     TPEGLMETVFKKAYKDLPEYLQGFAYTCAVMQDPDSLERIAYEFALDNAAEGVFYVEV
                     RFAPQLHVNDHMDMETVISSVNRGLERAKKDINKAKADPDMPDFEYGIIVCNLRMFMG
                     ESSPYYKKIMDALSWSPRKEIFAQASLQLARAAIDIRDRKGYPVVGFDLAGAEHGYPA
                     IDHMEAFHYAHRNFMKKTVHAGEAYGAESIFQAITALHADRIGHGYLLFSKEALTNPE
                     ITDKEAYLKKLSEYIADRRITIEVCLTSNRQTNPGLVDISKHSLRDMLKHNLSVTLCT
                     DNRLVSQTTVSDEIELAIKNFDIAPGTLRNIIVYGFKRSFFPGSYVQKRNYVRGVIDY
                     YEKICKKHGVASK"
     gene            complement(6371..7204)
                     /locus_tag="PLD82_02320"
     CDS             complement(6371..7204)
                     /locus_tag="PLD82_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG64255.1"
                     /translation="MNWKIPRGSCALVTGASSGIGRAVALGLAARGVHCVLAGRNRKR
                     LTELARVLSGDYGIETGILVQDLSQPGAGEKLYRAAKRAGYRIDILVNNAGAGLKAAP
                     QHGQDLADVRTLLALNCAAVLELATLCAADMAKNGRGWILNVASTASFQAMPWAALYG
                     ATKAFVLSLSEAMHVELFRSGVAVTAICPGITDTNFFKHGKPRVPGWLYPFLTPERVA
                     RIGLAALSRNAPVAIPAFRHWLFAQFPRVLPRALLLRLMGVIEGRRKGVRTRKTGGHT
                     S"
     gene            complement(7201..>7744)
                     /locus_tag="PLD82_02325"
     CDS             complement(7201..>7744)
                     /locus_tag="PLD82_02325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG64256.1"
                     /translation="QAGSLKEQAAIDALHDAKSRVQSMAVLYDRLYRSEGFVSVPINE
                     YLGQLVDDIVGMFSLRVPVRIHKEIASFVIDTRKLFAVGIIVNELITNCLKYAFVDRT
                     EGEILVSANRENQQAVLVVADNGRGLPEGFSPGRTGGFGLRLVDILVRQIEGSLTIER
                     KGPEGGARFVIAFSEAGACQ"
BASE COUNT         1620 a         2485 c         2200 g         1439 t
ORIGIN      
        1 gcagcaccgg tatagattcc tcgtaccatg ccattctttc ctttccggct gaacaccctc
       61 agcccttgtc atgcccgtcc cctctccgga gacgggaatc gttcacgatt ggtccttgac
      121 cctggtgatt cgtgctccga gcgccatcaa tttctcttcc agcttctcgt agcccctgtc
      181 aacgtggtag atccgtcgca ccagactggt cccctcggcc gccagggccg cgaccacaag
      241 ggccgcaccg gccctgaggt cggacgccat caccggggcg ccttccagtt tcggaacgcc
      301 acgcaccacg gccgtctggc cctcaagccg gatatcggca cccatccggt tgagctccgc
      361 cacgtggatg aaccgttcgg ggtagatctt ctcggtgatg accgaggttc ctccggcatg
      421 cgccagcaca gccatcatct gggcctgcat atcggtcgga tagcccgggt agggcagggt
      481 ttcaaaatcc accgaatgga tcttcgatcc ggcacgcagc cggattccgg cctgatccgc
      541 cacgatatcc atccccgccc gccggaaggt ctccaggacg atccccaggt gcgcaggatc
      601 ggccccgcgg acaaggacat ccccgccggt gcaggcggcc gcagccagga gggtcctggc
      661 ctcgatccgg tccggaatga tccggtagcg gcaaccatgc aaccggggaa cgccggttat
      721 ggtcaaggtg gtcgttccga tcccttctat ttttgcgccc atggccacca aaaaatgaca
      781 ggcatccacg acctcgggtt caatcgcggc cgactggatg acggtcactc cgtccgccag
      841 ggcggccgcc atcatcacgt tgtccgtggc cagcaccgaa gagccggccg aaccggccag
      901 gtacatcgtg gcgcccttga gcttgggaga gcaggcatgc acatatccat gggtgatctc
      961 gatctccgcc cccagggctt caagaccctt gaggtggagg tcgatcggac gggcgccgat
     1021 ggcacatccg ccggggaagg aaacccgggc ctcgccttct ttggcgagaa gtggtcccag
     1081 gaccgaaatg gaagcgcgca tggtgctgac gagctcgtac ggggcttcgg tggaggtcac
     1141 ccgcccggcg tggacgatga catcgccccc cctgcgttcg accttgcgcc caagcatctc
     1201 gagcaaccgg agcatggtcc ggacgtccct cagatcgggg acgttttcga tgatgcactc
     1261 gtcatccgtc aggagacagc ctgcaagaat gggcaatgat gaatttttcg aaccggctgc
     1321 agtggtgtca cccgcaatcc ggactggtcc ctcgatactg aatgcgtcca tggttcccgt
     1381 cgtaccccgt ttgtctgagt ctgccctgtt aacatcggcg ctccggaaaa aaacttgatt
     1441 gccgggcggg gggaatcgca gtgatgatcc tgcagggaga gtgcaggttt tcggaatttg
     1501 tatacgacac cccacctcct gctccggtcg atggtgccat cagaagagtc gtctcccttc
     1561 actccttcaa aatgggcgcc aaccagacag acttttccgt accgcacgca tcctgccggg
     1621 aacaacgctc ttgcctggta ctgtccgccg ggtgtatggt ttttctgccg cagtctaact
     1681 gcgccagatc caagggaggt tgcagtccta tgagcaatgc cacaccgata accgatgcca
     1741 cattccaatc ggaagtcatc aattcttcca ttcccgttct tgtcgatttc tgggccccct
     1801 ggtgcgggcc ctgccgtgcg atcggtccgg tcattgacga gattgcagcc gagcgggccg
     1861 gaaaactcaa agtctgcaag atgaatgtgg acgaagaaca ggaaaccgcc ggacaattcc
     1921 aggtcatgag catccccacc ctgatcctct tcaaggacgg gaacgtcgct gccaagatcg
     1981 tcggagcaca ggccaaggac aagctcctct cccagattga ccctctcttg tagagcaaaa
     2041 ttcgggtttc aagcggtcca aaatcgcctc cggggtgtcg atgataggga tacagccgga
     2101 ggtgtttcat gtcacgtctt gttcttcttg tttccgtcct cgtcacgctt gcaacctctg
     2161 tgcaggcagc caccaatgcg caagccggct cgtcacagtc acaagcaacc acaagtaccc
     2221 aggccagctc gtcacagtca caaacagcca ccagtaccca ggccaccaac cagtccggcg
     2281 ttgtttccag cgccaccaac cgccccccgc aggacctgaa gactgttccc aaaaaagaag
     2341 atcttgaata ctggcaggaa actatgctct acgggaccga gcagcagaag gattccgtcc
     2401 tcaaaaccat gcagtccgtc caggccaaac aggtagatga catccttctg gcggctctcg
     2461 ccaacgagtc cgagggcccg atccagctca agctggtcca gatcctctac gaccggagga
     2521 tcaccggagc cctcggcccc ctggttcgga ccctgaccaa tgcccgggag gcggacacga
     2581 tcggcccggc catcgctgcc ctggcccgtt tcaaggatcc cggcgtcctg ccctacatcc
     2641 tccagcatct gaccaatgag accctctccg tccagcaggc cgcggtccga gcgctcggag
     2701 agatcggcga tcccgcaccg ggcaagcggc tcctcgagat gctggacagc ctgccggccg
     2761 caagcgagct gcgctacgat ctggtcaatg ccctcggaca gatgaaatac ggaccggcct
     2821 acgagagtct gcgcaccatc gccatcaaca gcgggaatcc gcgctacctc cgggcctttg
     2881 ccatcaccgc actcggctcg atcggaaacc ggaagatcgt tcccgacttc atcaaactcc
     2941 tctccgaaga aaaaatcccg gacatcaagg tccggatcat cgccgccctg ggggagatgc
     3001 ccggaagcga atccgtccag gcgatccgga ttgccatgaa tgacggggat gaacggatcc
     3061 gggccagtgc catccaggca gcggccaagt caaaagacga agcctatctt cgccccctgc
     3121 tctataaatt ccgctacgac aatgaggccc gtgtcatgct ggccgctgcc gaagcccttg
     3181 tggagttgaa ataccccgaa ctgccggccc tgattctcgc aaaattcgaa tcaacccggg
     3241 atgtccacgt catgtcccgc tttgtcacca tcctcaaaaa actcccaccg caaaaacagg
     3301 ccattccaat gctccggcgc aaacaaaagg aaaattcctt cgccaaggtc aaggacgaga
     3361 tcgaagccct cttgaccctc tggggcgaga aagccgcaag cgacaaatct cccaagctgg
     3421 acgagaccaa aaaagcggcc actcctgaaa aaaaagccga ggacacgaag tcgggacagt
     3481 ggggagaccg gatctacctc aaggaatgat acctcagtcc ggcaggcgga tccagtcagg
     3541 ctgtccggcc agcggactct tgacaaacca gggtgtcgga tcgacacagc ggttcccgat
     3601 ccgcaattcg taatgcaaat ggggaccggt cgaaagcccg gtcgttccga ccaggcccac
     3661 gggacttccg gtcccgaccc tggcattcga gctggcgatc acccgggaga gatgcatgaa
     3721 ggtggagaac acgccgtccc catgatcgag aatcaccaga cccccatgga ccgctgtctc
     3781 atgggcaaag gcgacacgtc cctccatcgg cgcgacaacg tccacgccct cgggaaaagc
     3841 gaaatcgatc ccgcgatgcg cttttccgtt ggtcgatgtc aggtagcgga caaccccgaa
     3901 cggagaactg accctgtcat agcgggcaat cggaatcctg agcttcacag gccactcgcc
     3961 ccgctgctcc cccgacgccc ggtagatctt ctgcaagcgc cagtactccc gctccggctt
     4021 ccctccggag agaatcgact cttttttttg cagccagcgc tgccaccagt cggccggtgc
     4081 cgggggatag gccgatccgg ggaagggtct ggcctgccgc gcaagaaacc gcaggccctc
     4141 gcggtcctca cgaaatttct ccacaggcat ctgccacgcg cgcatgtacc aggcaccatc
     4201 ccggccatgg acccagacag ccaaccggag gatgccagcc ggcggcatgc agtccaggcc
     4261 ggccaaggcc gaatactccc gtccgcccgg acggggaacg aggataaggg aagcatcggc
     4321 cagcatcccg cccacccggg agaccgtgtt tgtggaacca agccggagtg ccagccgcag
     4381 gaacccaccg gcccgaagat tggtcgtgtc gacatcgagg ctgaagcgga gatcgtgaca
     4441 ctggtccccg tcctcgcgcg acgcaggcac cagccaggcc cgaccctcat caagccgcca
     4501 ccagtcctcc cggagcttga acagccggat gtcggcggac gggacggact gtgtcgccgt
     4561 cgtcacggag ggtccctcga ccacaagacg gccccgctcg agaacggaga ggacgagacg
     4621 ggctccgggt tcgagaagca cccgcacatc tccgtcatgc gtcttccccg gctccggtct
     4681 gaggtcaaag gcctgaccgg tttcggggtt gcgcccgacc caacgcaccc ctccctcgac
     4741 ggactcgact tcgagaaaat ccgtctgtcg aaggaggagc cagaggacac aagccaccgc
     4801 cccgagtacc accagggctc cggcgaaaaa gaccgcccgg gtccgtcccc gggcagatgc
     4861 ttcactgatc ggtttcatgc atcccttact tgctggcgac cccgtgcttc ttgcagatct
     4921 tctcgtaata atcgatcacg ccacggacat agttccgctt ctggacatag ctcccgggga
     4981 agaagctgcg cttgaacccg tagacgatga tgttgcgcaa ggtccccgga gcaatgtcga
     5041 agttcttgat agccagctcg atctcgtcgc tgacggtcgt ctgcgaaaca agccggttgt
     5101 ccgtgcagag ggtaaccgag agattatgct tgagcatgtc ccgcagggag tgcttgctga
     5161 tatccaccag gcccggattg gtctgccggt tggaggtcag gcagacctcg atcgtgatgc
     5221 gccggtcggc gatgtactcc gagagctttt tgagataggc ttccttgtcg gtgatctccg
     5281 gattggtcag agcttccttg ctgaagagga ggtagccatg cccgatccgg tccgcgtgga
     5341 gggccgtgat ggcctggaag atgctttccg caccataggc ttcgccggca tggaccgtct
     5401 ttttcatgaa attccggtgg gcatagtgga aggcttccat gtggtcaatc gccggatagc
     5461 catgttcggc accggccagg tcaaaaccga caaccgggta tcccttgcgg tcccggatat
     5521 cgatcgcagc ccgggccagc tggagtgaag cctgggcaaa gatctccttg cggggtgacc
     5581 aggagagggc atccatgatt tttttgtagt agggtgagga ttcgcccatg aacatccgca
     5641 gattacagac gatgatcccg tattcgaaat cgggcatgtc cggatccgcc ttggccttgt
     5701 tgatgtcctt ttttgcccgc tccagtccgc ggttgacact ggagatgacc gtctccatat
     5761 ccatgtggtc attgacatgg agctggggcg caaagcgtac ctcgacgtag aacacaccct
     5821 cggctgcgtt gtccagggca aactcgtagg caattcgctc cagactgtcc ggatcctgca
     5881 tcacggcgca ggtataggca aacccctgga gatactcggg gaggtccttg taggcctttt
     5941 tgaagacagt ctccatgagc ccctcgggtg tataggccgg aagggaaaca ttctgctcct
     6001 tggccagctc gatcagggtg gtcagtctga gggaaccatc aagatggacg tgcaagtccg
     6061 tcttgggaat cgcgcgtatg aactcgctgc tgaacacggg aatacctctc tttcacgtgg
     6121 aactcgtttc tgtattgcag acaagtgtag cagattccga cccgtcaagc aagaaccgag
     6181 attgcgcgcc gggtccagcc tggcgccccg cgcgaaatgg gcgatccatc gccatcacaa
     6241 tccgttgcca caatccggtt ctgtttttga tgaacaccct cagtcctgca acgcgcgccc
     6301 tggcggcacg tggatggtgc cgggatcccg acactcccgg agtctttgca ccattctgga
     6361 cgtccgggat tcaggacgtg tgaccaccag ttttcctggt ccgtacccct tttcgtcgtc
     6421 cttcaataac ccccatcaac cgcagaagca gggcccgggg caagacccgg gggaactggg
     6481 caaaaagcca gtgccggaag gccgggatcg cgacaggggc attgcgggag agggctgcca
     6541 gtccgatccg tgccacccgc tccggtgtca aaaaggggta gagccatccc ggcaccctcg
     6601 gtttgccgtg cttgaaaaaa ttggtatccg tgatcccggg acagatcgcg gtcacggcaa
     6661 ccccggagcg gaagagttcg acatgcatcg cctccgagag ggagaggaca aaggccttgg
     6721 tcgccccata gagcgcagcc cagggcatgg cctggaacga ggccgtcgag gccacattca
     6781 ggatccagcc cctcccgttt tttgccatat ccgcggcaca aagcgttgcc agctcgagga
     6841 cggctgcgca gttcaatgcc aggagcgtgc gcacgtcggc cagatcctgc ccatgctggg
     6901 gggcggcctt caatccggcg ccggcattgt tgacgaggat atcaatccgg tagcccgcac
     6961 gtttcgcggc ccggtagagc ttctcccccg cacctggctg cgagagatcc tggacaagga
     7021 tcccggtctc gatcccgtag tctccggaca agacccgggc cagttctgtc agtctctttc
     7081 tgttccggcc ggcaaggaca caatggacgc cgcgtgcggc aagtccaagc gccacagccc
     7141 gcccgatacc cgaggatgcc ccggtgacga gcgcacagga tccgcgcgga atcttccagt
     7201 tcactggcaa gcccccgctt cactgaacgc gatcacaaac cgggcaccac cctccggccc
     7261 tttgcgttca atcgtgaggc ttccctcgat ctggcggaca aggatgtcaa ccagacgaag
     7321 accgaagcct cctgtccgtc cgggcgaaaa accctccggc aatcctctgc cattgtcggc
     7381 aacaaccagg acagcctgct ggttctcccg gttcgccgaa acgagaatct cgccctccgt
     7441 ccggtcgaca aaggcgtatt tgaggcaatt cgtgatcaat tcattgacaa taatcccgac
     7501 ggcaaagagt ttgcgggtat cgatcacaaa agatgcgatc tccttgtgga tccggaccgg
     7561 aacccgcagg gagaacatgc cgacaatgtc atccaccagc tggccgaggt actcattgat
     7621 cggaacactg acaaatcctt cggaacgata gagtctgtcg tacagcacag ccatgctctg
     7681 gacccggctc ttggcatcat gcaaggcatc gatggccgcc tgttccttga gactcccggc
     7741 ctgg
//
LOCUS       DAOSHE010000046         7714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8182_length_7714_cov_4.433608, whole genome shotgun sequence.
ACCESSION   DAOSHE010000046 DAOSHE010000000
VERSION     DAOSHE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8182_length_7714_cov_4.433608"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="lon"
                     /locus_tag="PLD82_02330"
     CDS             <1..1323
                     /gene="lon"
                     /locus_tag="PLD82_02330"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG64257.1"
                     /translation="RGSILCLVGPPGTGKTSLAMAMAEALGRKFVRMSLGGMRDEAEI
                     RGHRRTYVGAMPGKIIQHLRKVGTSNPVFLLDEIDKLGNDYRGDPSSALLEVLDPEIN
                     NAFQDHYLEVEYDLSQVFFVTTANSIHGIPGPLADRMEVITIPGYTEAEKRAIAMRYL
                     VPRQFERNGLKTGDLEILPDAIERLIDGYTREAGVRGLEREIQRICRKQALAILEKKS
                     KSHTRIDATALARVLGKPRHKNSEMLRIDRPGLVHGLAWTAVGGQVLDIEARASRGQA
                     RLLLTGKLGSVMQESAQTALSLVRFMLDNKTETNFSETDFHVHVPEAATPKDGPSAGI
                     TIASAIYSAITGRIAPSDIALTGEVSLLGRVLPIGGLKEKVLAAKRLGVQRIIIPDGN
                     RPDLEEIDREIRKGLVFLPVRDFEEVVKELFPQKGKGPAQGTTRKNPG"
     gene            1338..2930
                     /gene="serA"
                     /locus_tag="PLD82_02335"
     CDS             1338..2930
                     /gene="serA"
                     /locus_tag="PLD82_02335"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOG64258.1"
                     /translation="MKHKILISDSFSKEGIEILTREPGFDVIYTPGLNEDQLCEAIRD
                     VDALIIRSASRVTARVLASAEKLALVARAGVGTDNIDKKAATDRGVIVMNAPSGNSVS
                     TAELALAHIFALARHIPQANASMKAGIWEKKKYNGVQLAGKTIGIIGLGRIGSNLARR
                     CRACDMRVIGSDPFLSDEQITMIGVEPVELDVMWKTADVISVHVPMTEKTRGLIGERE
                     IAMMKPTTMLVNCARGGIMDEAAVAAAVSSGKLAGAAFDVFTEEPPKDSPFVAVENIV
                     MTPHLGASTEEAQVEVAIETAQEIVDYFTSGMIKNSVNMPAMDAATLSVMQPWIRLSE
                     QMGSFMAGLANTGVTGVVLTYAGDLADKQVWFLTQSALKGLLDPVLSNEVNFVNAPVM
                     AKERGIQVSERRESIPNESGPVLSLQVTTSNGEVHELRGTVSGSDIRCTSLDGFAFIL
                     EPAGTILVVHNRDVPGVVGLLGTKLGNAGVNIASMRLGRRSKGSEVLTILSIDGDVPE
                     TVLAEIRNEAPIVDARVIVLPQ"
     gene            2939..3391
                     /locus_tag="PLD82_02340"
     CDS             2939..3391
                     /locus_tag="PLD82_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG64259.1"
                     /translation="MDGITFKRISRRGLWEIEPLWQRLNALHADVSPHFGDLHENLTF
                     DRRMGGLFRLEDQDLHILAACSESRMIGYVVAVVYEGRGEIESLFVDEAFRRRGIGRE
                     LCVRSMSWMRERECRTMVVAVSFGNESVIPFYRSLGFCERMVYLQHKE"
     gene            complement(3407..4363)
                     /locus_tag="PLD82_02345"
     CDS             complement(3407..4363)
                     /locus_tag="PLD82_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HOG64260.1"
                     /translation="MKNTVFAALAIILSVSLSAATRQEESRLRLHLAGTFSGQAWIAD
                     LVVRSDAATVTARMANGMEIQLEGRLMDTQIVFTSTNKAILPGSITADIPDGTTAIFR
                     PVWTWRSQRQEFPARVVARERVVGDFRENRHEASCAWPVVGGYNTYANTTITAILSNF
                     AVSNHAAFLKSSADPEERDGNTGLWAHHLGYDLYAATERFVSVRFALYTYTGGAHPGR
                     WTHSFNFAISGESARVLRLGSLLRTGMLEPLLRLVQDKLREKGASWPNEVNGGHLENF
                     CITPTGLDFHFDPYIAGSYAEGDYSVFLAWNELEQFVSAGVR"
     gene            4475..6100
                     /locus_tag="PLD82_02350"
     CDS             4475..6100
                     /locus_tag="PLD82_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG64261.1"
                     /translation="MTHKMHTVFILVSSLIVSLGIFASCNSKDQQAMLRQEREYTEAV
                     RHARAPSMNGDGLAKSKMDGMPAGIEHDREQYEKRTENGFFDAARIPLSTFSIDVDTA
                     SWANIRRFLQSGRLPPKDAVRLEECVNYFPYAYEGPSDGRPFAVHAEVADCPWAEGRQ
                     LVRIGVQGKHISLENLPPANLVFLLDVSGSMFSENKLPLVKNALSMLTRKLRPQDRVS
                     IVVYAGAAGLVLPPTKGSDKETILGALDRLQAGGSTAGGAGIKLAYKVAEENFDRNGN
                     NRVILCTDGDFNVGASSDGEMVRLIEEKRATGVYLTILGFGMGNYQDAKIQKMADAGN
                     GNYSYIDSAMEARKVLINQMGGTLLTIAKDVKIQVEFNPAYVGSYRLLGYEKRMLRAE
                     DFRDDKKDAGELGAGHSVTAIYEIVPKGGANPSGYVEPLRYATNAVSTGVLNNELVTV
                     KLRYKLPGNDTSTGFSIPVRTGSTRIDKASQEMRFAVSVAEAALLLYGSEFKGKADWN
                     TLIDRANSSLGRDEEGYRAEFIRLAKLAAGMQD"
     gene            complement(6398..>7714)
                     /locus_tag="PLD82_02355"
     CDS             complement(6398..>7714)
                     /locus_tag="PLD82_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64262.1"
                     /translation="LTEFSYDCVTQELTLAGTMQDRWVNPLGLSRVFKNREGVVFENA
                     AFEIGWIPGSPTPTKIGFGIETAKIMDLVFGAVVDFSPTDGQLAFQAKVNKLNYSDLL
                     KIITRVFGKRMPNVIPEDLALNDVEILFSPNGGKVGQLEIAQGFAFKGEARIGSALNG
                     KIDFTANFDDGLQFSVEMNNSIREILLKRVRNHKALKVLVNRVTSTLNLRRIAIDLKA
                     MRSEFGGGVACDLTVFGKRINFNLSGSFNPDRLVNSLLDKIVDMAGDEFKAAYRAVGN
                     AAKAAGNKGKQVAMRAAQEAGQMAHDVKTSVKHSGHSKNKCMNDCVPHYANGQRRKMR
                     DGTNEAVREFYQEALREIRGVEGETPEQTRKLREMMLGSEWNDLLRDIDDKWKRVHND
                     DEVKNYFVRDNSEKQAINKYRGLINEYWNNHKNYRTALWNQLMNAR"
BASE COUNT         1741 a         2234 c         2122 g         1617 t
ORIGIN      
        1 cgtggttcca tcctctgcct cgtcggtcca ccgggaaccg ggaaaacgag tctggccatg
       61 gccatggccg aggcactcgg aagaaaattt gtacgcatgt ctctgggtgg aatgcgagat
      121 gaggcagaga tccgaggaca cagacggacc tatgtcggag ccatgccagg aaagatcatc
      181 cagcacctgc gcaaggtcgg gacctccaac ccggtcttcc tgctcgacga gatagacaaa
      241 ctgggcaatg attatcgtgg cgatccttcc tccgctttgc tggaagtcct tgaccccgag
      301 atcaataacg cgtttcagga tcactacctt gaggtggaat acgatctctc ccaggtcttt
      361 tttgtgacaa cggcaaattc catccacgga atcccgggcc ccctcgcaga ccggatggaa
      421 gtgatcacca tcccgggata caccgaggcc gagaaacgcg ccatcgccat gcgctatctt
      481 gtcccacggc agttcgaacg gaacggactc aagaccggag acctcgagat ccttcccgat
      541 gccattgagc gtctcatcga cgggtatacc cgcgaggccg gggtgcgcgg gttggaacgg
      601 gagatccagc ggatctgccg aaaacaggcc ctcgccatcc ttgaaaaaaa gagcaagtcg
      661 cacacccgga tcgacgccac ggcgctggca cgggtcctcg gcaagccacg ccacaagaac
      721 agcgagatgt tgcggatcga caggccgggc ctggtccatg gactggcctg gaccgcagtg
      781 ggcggtcagg tcctggatat cgaagcccgg gcctcccgcg gccaggcccg tctccttctg
      841 acgggaaaac tgggctcagt catgcaggaa tccgcacaaa cggccctctc gcttgtacgc
      901 ttcatgctcg acaacaaaac tgaaaccaat ttctccgaaa ccgattttca tgtccacgtt
      961 cccgaagcgg caaccccgaa ggatgggccc tcggccggca taaccattgc cagcgcgatc
     1021 tattccgcaa tcaccggcag gattgctccg tcggatattg ccctgaccgg cgaggtctcc
     1081 cttcttggac gggtcctccc gatcggcggg ttgaaagaaa aggtcctggc tgccaaacgc
     1141 ctcggtgtcc agaggatcat cattcccgat ggaaaccgtc ccgatcttga agagattgac
     1201 cgcgagatac gcaagggact cgtatttctc cccgttcgtg attttgaaga agtcgtcaag
     1261 gagctgttcc cccaaaaagg caaggggcca gcccaaggaa caacccggaa gaatccgggt
     1321 tgatgaaagg actcatgatg aaacataaaa tactgatcag cgacagtttt tccaaagaag
     1381 gaatcgaaat cctcacgcga gagccgggat ttgacgtgat atacaccccc ggactcaacg
     1441 aagaccagct ctgcgaggcg atccgggatg tcgatgccct catcatccgc agcgcgagcc
     1501 gtgtcacagc gcgcgtgctg gcatcagccg aaaagctcgc cctcgtagcg cgggccggtg
     1561 tcgggacgga caacatcgac aagaaggccg ccacggaccg cggcgtgatc gtcatgaacg
     1621 cacccagcgg gaattcggtt tcgacagccg aactggcctt ggcgcatatt tttgcgctgg
     1681 cacggcacat tccccaggcc aatgcctcca tgaaggccgg catctgggaa aaaaagaagt
     1741 acaacggtgt ccagcttgcc ggcaagacca tcggcatcat cggattgggc cggattggaa
     1801 gcaatctggc acgtcgctgc cgggcctgtg acatgcgcgt catcgggtcg gatccgtttt
     1861 tgagcgatga gcagatcacg atgatcgggg ttgaaccggt cgaacttgat gtaatgtgga
     1921 agaccgcgga tgtcatctcg gttcacgttc cgatgacgga gaaaacacgg ggtttgatcg
     1981 gcgagcggga gattgccatg atgaaaccca caacgatgct cgtcaactgc gccagaggcg
     2041 ggatcatgga cgaggctgcg gtggccgcgg ccgtttcgtc cggaaaactt gccggtgccg
     2101 cctttgatgt ctttaccgaa gaacccccaa aggattctcc ctttgtcgcg gttgaaaata
     2161 tcgtcatgac cccgcatctt ggagcctcaa ccgaagaagc ccaggtcgag gtggcgatag
     2221 aaacagccca ggagatcgtg gattatttta ccagcggcat gatcaagaat tccgtcaaca
     2281 tgcccgccat ggatgcggcc accctgtcgg tcatgcagcc ctggatccgg ctctccgagc
     2341 agatgggaag tttcatggcc ggcctggcca acaccggcgt tacgggggtc gtcctgacct
     2401 atgccggaga tctcgccgac aaacaggtct ggttcctgac ccaatcggct ctcaagggac
     2461 tcctggatcc ggttctttca aacgaggtca atttcgtcaa tgcaccagtc atggccaaag
     2521 aacgcgggat ccaggtctcg gagaggcggg aatccattcc caacgaatca ggcccggttc
     2581 tcagcctgca ggtcacaacc tccaacggag aggttcatga acttcgggga acagtctcag
     2641 gcagcgatat ccgctgtacg agcctggacg gttttgcctt catcctggag ccggccggaa
     2701 caattctggt cgtccataac cgggacgtcc ccggcgtggt cggcctgctc ggaacaaaac
     2761 tgggaaatgc cggcgtgaac attgccagca tgcggctcgg ccggcggtca aaagggagcg
     2821 aggttctgac gattctctcc atcgacggag atgttcccga aacagtcctg gccgaaatca
     2881 ggaacgaagc gccgatcgtc gatgccagag tgatcgtgtt gccgcaataa attctacaat
     2941 ggacggaatc accttcaagc ggatctcccg gaggggcctc tgggagatcg agccgctctg
     3001 gcagcggctg aatgccctgc atgccgacgt ttccccccat ttcggggacc tccacgaaaa
     3061 tctcacgttt gaccggagaa tgggcgggct cttccgcctt gaggatcagg atctccacat
     3121 cctggccgcc tgctcggaat cccgcatgat cggctacgtc gtagccgtgg tctatgaggg
     3181 tcgtggcgaa atcgagtccc tgtttgtcga cgaagcattc cggcgacggg gaatcggccg
     3241 ggaactctgt gtccggtcca tgtcatggat gcgggaacgg gaatgccgca ccatggttgt
     3301 cgctgtctcg ttcgggaatg aatccgtcat tcctttttac cgatcgctcg gtttttgcga
     3361 gcggatggtc tatctccagc acaaggaatg acggattctc cccggattac cggacacccg
     3421 cagaaacaaa ttgctcaagc tcgttccacg ccaggaaaac cgaataatcc ccctcggcat
     3481 acgaccctgc aatataggga tcgaaatgga aatccagacc ggtgggtgtg atgcaaaaat
     3541 tctcgagatg cccgccgtta acctcgttcg gccagcttgc gcccttctcc ctgagtttgt
     3601 cctgcaccag tctgagaagc ggttcaagca ttcccgtccg gagaagactg ccaagcctca
     3661 ggacccgtgc cgattctccg gagatggcaa aattgaacga gtgcgtccat cttcctggat
     3721 gggcacctcc ggtgtaggta tacagggcga aacgcacgct gacaaaccgt tctgtcgccg
     3781 catacaggtc atacccgaga tggtgtgccc aaagtccggt atttccgtcc cgttcctccg
     3841 gatccgctga actcttgagg aaggcagcat gatttgaaac ggcaaaattc gagagaattg
     3901 cggttattgt ggtattggca tacgtgttat acccgcccac aaccggccag gcgcaggagg
     3961 cttcgtgacg attctcccga aaatcgccga ccacccgctc ccgtgcaacc acccgggccg
     4021 gaaactcctg cctttgtgac cgccaggtcc agaccggacg gaagattgcc gttgttccgt
     4081 ccgggatatc tgccgtgatg cttcccggca ggatcgcttt gtttgtcgac gtaaacacga
     4141 tctgcgtgtc cataagccga ccttccaact ggatctccat tccgtttgcc atccttgccg
     4201 tcacagtcgc agcatcgctg cgcacaacca gatcggcaat ccatgcctgt ccggagaacg
     4261 tcccggcgag gtgaagcctg agtcgggatt cttcctgacg ggttgcggca ctcaagctga
     4321 cagaaagaat gatggccaaa gcagcaaaga ctgtgttttt catggtcgtt tctccagatt
     4381 ctcaaggcgg acagggttga ggacaccgtt ttttggtgaa cattctcgtg ccctgtcagg
     4441 taagtggtat atcatccata acaggagcgt acatatgaca cacaaaatgc acaccgtatt
     4501 catcctggtc tccagcctca ttgtcagcct tggaatcttt gcctcatgca actcaaaaga
     4561 ccaacaggcg atgttgcgcc aggagcgtga gtacaccgaa gcggtccggc atgcccgggc
     4621 accatccatg aatggcgacg gccttgccaa atccaagatg gacggcatgc cggccggcat
     4681 cgaacacgac agggagcagt acgagaagcg tactgaaaac ggctttttcg atgctgcccg
     4741 tatcccgctt tcgactttct ccattgatgt ggacacggcg tcctgggcca acatcagacg
     4801 ctttttgcaa tcaggccgac ttccaccaaa agatgccgtc cgtcttgaag aatgtgtaaa
     4861 ttacttccct tatgcctacg aggggccaag cgatggtcgt ccctttgccg tgcatgccga
     4921 agttgccgac tgtccctggg ccgaaggccg tcagctcgtc cgaattggcg tccagggaaa
     4981 gcatatctct ctggaaaacc ttcccccggc gaaccttgtt ttcctcctgg atgtctccgg
     5041 ctccatgttc tcagaaaaca agctgcccct tgtaaaaaat gccctctcga tgctgacacg
     5101 gaaactcaga ccgcaggatc gcgtttcgat tgttgtctat gccggagcag ccggactggt
     5161 tcttcccccg accaaaggga gtgacaaaga gacaattctt ggcgcactgg accggctgca
     5221 ggcgggcgga tcaacggctg gcggagcagg catcaaactc gcctacaagg tcgctgaaga
     5281 gaattttgac cggaacggaa acaaccgggt catcctctgc acggacgggg atttcaacgt
     5341 cggagcttcg agtgacggag aaatggtccg gctcatcgaa gaaaagcggg ccaccggcgt
     5401 ttacttgacg atccttggct tcgggatggg aaattaccaa gatgcaaaaa tccagaagat
     5461 ggctgacgcc ggaaacggaa attattcgta catcgactcc gccatggaag cacgcaaagt
     5521 cttgatcaac cagatgggcg gcacgctctt gacgatcgcc aaagatgtca agatccaggt
     5581 cgaattcaat ccggcctacg tcggctccta cagacttctc gggtacgaga agcgcatgct
     5641 tcgtgccgaa gatttccgtg acgacaaaaa ggatgccgga gaactcggtg ccggacatag
     5701 cgtgacagcc atctatgaaa tcgtccccaa gggcggcgcc aatccttccg gatatgtcga
     5761 gcccctgcgc tatgcgacga acgcggtctc gacaggcgtc ttgaacaatg aacttgtgac
     5821 cgtcaaattg cgctacaaac ttccgggcaa tgacacgagc accggtttct ccattcctgt
     5881 ccggacaggt tccaccagga tcgacaaagc ctcccaggaa atgcgcttcg ctgtttcggt
     5941 ggccgaagca gccctgctcc tgtacggttc ggaattcaaa ggcaaggctg actggaacac
     6001 cctgatcgat cgtgccaata gcagtctcgg ccgggatgag gaagggtatc gcgcagagtt
     6061 tatccgtctg gccaaacttg ccgccgggat gcaggactga gcggaattcc acgcactgcc
     6121 ggatccggtc cggactgagg atgttcacca aaacaacgac tctctggatg ccattccatc
     6181 cggggagtct ctgttccatc caaacacctc gcaatcatcc atcctgcgga cgcaaccctg
     6241 agcgttcagc ctgaccatgc ctggcctgcg gagacgatcg cgtcaggcga tcaagcaagg
     6301 cgaagcaccc ggggccggcc aggcattcgt gggcagacaa aacgcaaccc ctgtgggagc
     6361 aggggctgcg ttcgctacag atatggacag gacaggatca gcgggcattc atcaactggt
     6421 tccagagagc agtgcgataa ttcttgtggt tgttccaata ttcgttgatc aagccgcgat
     6481 atttgttgat ggcctgtttt tcggagttgt cccgcacgaa gtagtttttg acttcatcgt
     6541 cattatggac tctcttccat ttatcatcga tatcacggag caagtcgttc cactcacttc
     6601 cgagcatcat ctccctgagc ttacgcgtct gttctggtgt ttccccctcg accccgcgta
     6661 tttccctcaa tgcctcctgg taaaactccc ggacggcctc gttggtcccg tcccgcatct
     6721 tgcggcgttg accgttggcg tagtggggaa cacaatcatt catgcacttg tttttggagt
     6781 gaccggaatg cttgacactg gttttgacat catgagccat ctgtccggct tcctgggccg
     6841 cgcgcatggc aacctgcttc cctttattgc ccgccgcttt ggcggcattc ccgacggcac
     6901 gataggccgc cttgaactcg tctccggcca tatcgacgat cttgtccaga agcgaattga
     6961 ccaacctgtc cggattgaac gaaccggaca gattgaaatt gatccgcttg ccgaacaccg
     7021 tcagatcaca ggccacacca cctccaaact ccgaacgcat ggccttcaga tcgatcgcga
     7081 tccggcgcag attgagggta gatgtgaccc ggttgaccag gaccttgaga gccttgtggt
     7141 tacgcacccg tttcagcaag atctcgcgaa tggaattgtt catctcgacg gaaaactgga
     7201 gaccatcgtc aaaattggcc gtaaaatcga tcttcccgtt gagcgcactc ccgattctcg
     7261 cttctccctt gaaggcgaac ccctgtgcaa tttcgagctg tccgactttg ccaccgttgg
     7321 gcgagaagag tatctcgaca tcgttaaggg caagatcctc ggggattaca ttcggcatgc
     7381 gtttcccgaa gacccttgtg atgatcttga ggagatccga gtagttgagt ttgttgacct
     7441 tggcctggaa ggccaactgt ccgtccgtcg gtgaaaaatc gacaacagcc ccgaagacaa
     7501 gatccattat cttggcagtt tcgattccga atccgatctt ggtcggtgtc ggagatccgg
     7561 gaatccagcc gatctcgaag gccgcgtttt caaacaccac accttcacga tttttgaaga
     7621 ctctcgacag tccaagcgga ttcacccagc gatcctgcat ggttccggcg agtgtcaact
     7681 cctgggtcac gcaatcatac gagaattcgg tcag
//
LOCUS       DAOSHE010000047         7649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8272_length_7649_cov_3.809982, whole genome shotgun sequence.
ACCESSION   DAOSHE010000047 DAOSHE010000000
VERSION     DAOSHE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_7649_cov_3.809982"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /gene="pnp"
                     /locus_tag="PLD82_02360"
     CDS             complement(<1..1849)
                     /gene="pnp"
                     /locus_tag="PLD82_02360"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG64263.1"
                     /translation="MSIEQVSFRVGDEEIVLETGRMGKQANGFIFARQGGTVVISSAV
                     AVEGKGGLDYFPLQVHYIEKFYAAGKIPGGFIKREGRPKDKEILISRLIDRPMRPLFP
                     KGYMNEVQILPTTVSVDKVNPPDILAMIAASASLSVSDIPFEGPLGSCRVGLIGGRFV
                     VNPTVQQIEESRLNLVVSGTDDAIMMVEGHADNISEDEFLKAMEVAHDAIRQVTAGVR
                     ALVEKVARPKKPFAPVMIAPALEQEVRAYATDAMRVASANPDKLARYAAIDEVVASTV
                     QHYTEAGKSEEDLKQMPMILHDLEQSIVRHEIVEEGKRPDGRAFDEIRQITIETSVLP
                     RTHGSALFTRGHTHALVTATLGTALDAQRLDDIEGDREKRFMLHYNFPPFSVGETGRI
                     GATGRREIGHGMLAERALAGIIPDSVSFPYVVRIVSEILESNGSSSMASVCGGCLALM
                     DAGVPVKDVVAGVAMGLVMGDNGKYSILSDIQGVEDHLGDMDFKVTGTRTGITAFQMD
                     IKVKGITFQIFRDALDQARMSRLFILDKMVEALPAARPDISKYAPRILSMKIDEDKIG
                     AVIGKGGETIRGIQESTETVINIADDGTITVASPDLTNAEKAIAIIKGLT"
     gene            complement(1946..2218)
                     /gene="rpsO"
                     /locus_tag="PLD82_02365"
     CDS             complement(1946..2218)
                     /gene="rpsO"
                     /locus_tag="PLD82_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG64264.1"
                     /translation="MSSTTVESKQAILKEYGIHEKDTGSPEVQVALLSGRISSLTEHF
                     KFHKKDHHSRRGLLKLVGQRRRLLNYLKDKDIERYRSIVSRLGLRK"
     gene            complement(2334..3266)
                     /locus_tag="PLD82_02370"
     CDS             complement(2334..3266)
                     /locus_tag="PLD82_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOG64265.1"
                     /translation="MMRIRDFDEWHRMRPKRVVYTVGNFDGVHLGHQRLIEVLLGMAR
                     ECGAVPVVVTFTGHPQQLEKKMDMPFLLTTSQARAELLSSFGIEVVFEPVFSEHLAAM
                     EPETFLERLAGEGSVGFVVGDDFRFGAARRGGVEDVAGFLERTGNGRLAIVPGLQMDG
                     DRISSTRIRSALARGDVALAARYLGREYRMDGTRKPGDRIGSSIGFPTVNLHGMETML
                     PANGVYLGSLQSAGLRYDAMLYIGVRPSVGGSEQRVEAHVLHAAPEIPPGASVSVSFR
                     DRLRDEAVFADREALRAQLERDREEAKARLVASR"
     gene            complement(3263..4177)
                     /gene="truB"
                     /locus_tag="PLD82_02375"
     CDS             complement(3263..4177)
                     /gene="truB"
                     /locus_tag="PLD82_02375"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG64266.1"
                     /translation="MPFLRAAVLPVDKPVGMTSFDVLRILKKVFRRVRIGHAGTLDPA
                     ASGLLLVCFGAATRLVSWFHTFPKTYRADVLFGVTTDSDDLDGSVTARNPVPAGLEQL
                     LPALYDEFRGEIRQLPPAVSAVKTGGKRAYALVRAGIAPELRERSVTVFELNERERGT
                     DRVALEIICSSGTYIRSIARDMGSRLGCGACLAGLRRTSLVGYRLEEAWKVSENPAQE
                     DILPFIRPLESCFVPKSSVALTPEESNRVAHGVLPWSLLPQLGDRGPGPLVLLNPAGQ
                     ILAVIAKEGERWQFIVVLPPNEAGGVAG"
     gene            complement(4179..4535)
                     /gene="rbfA"
                     /locus_tag="PLD82_02380"
     CDS             complement(4179..4535)
                     /gene="rbfA"
                     /locus_tag="PLD82_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG64267.1"
                     /translation="MAVSHRPERVARIVQQEISRLLHEEVKDPRIAGAVFTIHEVRMA
                     RDLGSADVYFSVLDSSSLGQVVPALERVTGFLRSRLSGVLDLRRVPELRFHVDRTIEE
                     GMRLDRIIEEANKPGE"
     gene            complement(4541..6490)
                     /gene="infB"
                     /locus_tag="PLD82_02385"
     CDS             complement(4541..6490)
                     /gene="infB"
                     /locus_tag="PLD82_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG64268.1"
                     /translation="MGMGFPASSDRRQPHKGGVTRGKEKEKRDRLPDKRWNDDYDVVR
                     KTSFKKKTKRETQLESVPEKIEIMETLTVSELARKMNLPAKEIISKLMTMGLMTTINQ
                     QIDADTATLVASEYGCTVKQVSLYDETVIDEIVDSPDRIVSRPPIVTVMGHVDHGKTK
                     LLDAIRSANVVDTESGGITQHIGAYQVHTPHGAITFIDTPGHEAFATMRARGAKVTDI
                     VILVVAADDGVMPQTIEAAKHAIEAGVPIVVAINKMDKPEANIDRVKQQLADRLDLLP
                     EDWGGKTMCVPVSAIQKKGIPELLEAVLLQAEMLELTADPDKLGMGSVLEARIDPGKG
                     TTVTVLCLGGSMSVGDNFVAGVHYGRIRAMYNDRGEKLEKIGPAMPVEILGSSGLPQA
                     GDPIHIVESEKRARTISGKRLELRRFEDTKTVKKVTMENIFDKIKAGETKELKVIIKA
                     DVQGSAEALKDSLEKIENSEVRLTCIRYSAGAINEDDVMLAATTKAIIIGFHVRPNTR
                     AREMAEREHVEIRPYTIIYDAINDIDSALRGMMTPELHEKVSGSAEVRDTFKVPKAGT
                     IAGCYVIEGSILRNNKVRVIREGEVIFEGKVSSLKRFKDDAREVQKGYECGIGVEGYD
                     DIRVGDILENYSMQEVTPGRGGKTV"
     assembly_gap    6784..6883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6884..7139
                     /locus_tag="PLD82_02390"
     CDS             <6884..7139
                     /locus_tag="PLD82_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64269.1"
                     /translation="RSVSLRAIPVAGAVSLRATSVAGAVSLPLRATPVPGAITLRATS
                     VAGTVSLRALFLLYGSFNRTRRFRGWCGEISNQLGCLRES"
BASE COUNT         1580 a         2292 c         2042 g         1635 t
ORIGIN      
        1 aagtcagacc tttgatgatg gcaatcgcct tttcggcgtt ggtcagatcg ggcgaggcta
       61 cggtgatggt accgtcatcg gcaatattaa tgacagtctc tgtactctcc tggatcccac
      121 ggatggtctc accacccttt ccgataaccg caccgatctt gtcctcatca attttcatgg
      181 agagaatacg tggtgcatac tttgaaatat cgggccttgc cgccggaaga gcctcgacca
      241 ttttgtcgag aatgaacaac cggctcatgc gggcctgatc cagggcatcc ctgaagatct
      301 ggaaggtgat tcccttgacc ttgatatcca tctggaaggc cgtgataccg gttctcgtac
      361 ctgtgacctt gaaatccata tcgcccagat gatcctcaac gccctggata tccgagagaa
      421 tgctgtattt tccgttgtct cccatcacaa gccccatggc gacaccggcc accacatcct
      481 tgacgggaac gccggcatcc atcagggcga gacagccacc acagaccgaa gccatcgagg
      541 aggaaccatt ggactcgagg atttcggaga cgatccgcac aacgtagggg aagctcacac
      601 tgtcggggat gatcccggcc agggcccgtt cggccagcat gccgtgtccg atctcgcgac
      661 gaccggtcgc accgatccgt ccggtctcac cgaccgagaa gggcgggaaa ttgtaatgca
      721 gcatgaaccg tttttcgcgg tcaccttcga tatcatccag acgctgggca tccagcgccg
      781 taccaagtgt cgccgtgacc agagcgtgtg tgtggccgcg ggtaaagagg gcacttccat
      841 gggtgcgggg aaggacagag gtctcgatcg tgatctggcg gatctcgtcg aaggcccgtc
      901 catcggggcg cttcccttct tcgacaatct cgtggcgcac aatcgactgc tcgaggtcat
      961 gcagaatcat gggcatctgc ttgagatcct cttcggactt gcccgcttcg gtgtaatgct
     1021 gcacggtcga agcgacaact tcgtcgatgg ccgcataccg ggcaagtttg tccggattgg
     1081 cactggccac cctcatggca tcggtcgcgt acgcgcgaac ttcctgctcg agagcaggag
     1141 caatcatcac gggggcaaag ggctttttgg gccgtgcaac cttttcgaca agggcacgaa
     1201 cgccggctgt cacctgacgg atggcatcat gcgccacttc catggccttg aggaattcat
     1261 cctcgctgat gttgtcggca tgcccctcca ccatcatgat ggcatcatca gtaccggaga
     1321 ccacaaggtt gagtctggat tcttcgatct gctggacagt cgggttgacg acaaagcggc
     1381 caccgatcag accgacacgg catgacccga gcggaccttc gaagggaatg tccgaaaccg
     1441 agagggatgc cgaagcggca atcatggcca gaatgtccgg cggattgact ttatccaccg
     1501 acacggttgt gggcaggatt tggacctcgt tcatgtagcc tttgggaaag agcggacgca
     1561 tgggccggtc gatgagccgg gagatcagga tctccttgtc ctttggtctg ccctctcgtt
     1621 tgatgaagcc gccggggatc ttgccggctg catagaattt ttcgatgtaa tgaacctgga
     1681 gcgggaaata gtcaagccca cccttgcctt caacagcaac agccgagctg atcacgaccg
     1741 tgccgccctg acgtgcaaag ataaatccgt tggcctgctt gcccattctt cctgtttcga
     1801 gaacgatctc ctcgtcaccg acacggaagc tcacctgctc tatgctcata cccgttcctt
     1861 gtttctgcat gcatggaatc gcgcgccgga aaagcacagc gcgcactgaa tcacagtact
     1921 gataaaaaac acaacccgca ggatgttact tgcgaagacc gagacggctc acgatggaac
     1981 gatagcgctc gatatccttg tccttgaggt agttgagcag gcggcggcgc tggccgacca
     2041 gcttgagcag tccacggcgg gaatgatggt ccttcttgtg gaacttgaag tgctcggtca
     2101 gactggaaat ccggcctgac agaagcgcca cctgaacctc gggtgatcct gtatccttct
     2161 cgtgaatgcc atattccttg agaatggcct gtttgctctc cactgtcgtc gaagacatat
     2221 atcctccata agatcgacgc ttctgtacgt gccgtaatcg ggcacagcta tcatcgtatt
     2281 ttgagaaaaa tactcattca ctgcaaaaag agcaatacga agcgagaaaa aacttatcgg
     2341 ctcgccacca atcgggcttt ggcctcttcc ctgtctctct cgagctgcgc ccgaagggcc
     2401 tccctgtcgg cgaaaaccgc ttcatccctg agacgatccc gaaacgagac ggaaacgctg
     2461 gcaccgggtg gaatctcggg agccgcatga agaacatggg cctcaacccg ttgctcgctt
     2521 ccgccaacac tcggccggac accgatatag agcatggcat cgtagcgcaa acctgctgac
     2581 tgcaaggatc caaggtagac cccgtttgcc gggagcatgg tttccatccc gtggagattg
     2641 actgtcggaa agccgatcga actcccgatc cggtcacccg gctttcgggt accatccatc
     2701 cggtattccc gtcccagata ccgcgcggcc aaggcaacgt ctccacgggc gagggcggaa
     2761 cgaatcctgg tggaactgat ccggtctcca tccatctgca gaccgggaac aatggccagt
     2821 cgaccgtttc ctgtccgttc caggaaaccc gccacatcct cgaccccgcc ccgtctggcc
     2881 gccccgaacc ggaaatcatc cccgacaaca aatccgacag atccttcacc ggccaatcgt
     2941 tcaagaaagg tctcgggttc catcgctgcc aggtgctcgc tgaacacagg ctcaaacaca
     3001 acctcaatcc cgaaggagga cagaagttca gcccttgcct gagacgtcgt caggagaaat
     3061 ggcatatcca tttttttttc caactgctgc gggtgccctg taaaggtcac cacaaccggg
     3121 acagcgccac attcccgggc cattcccaga aggacctcga tcagtctttg atgtccaagg
     3181 tgcacaccat cgaaattccc gacggtatag acaacccgct tgggccgcat cctgtgccat
     3241 tcatcgaaat cccgaattcg catcatccgg caactccacc agcctcgttc ggaggcagca
     3301 cgacgatgaa ttgccagcgt tccccctctt tggcaatgac ggccaggatc tggccagcgg
     3361 gattcaacag cacaagtggc cccggccctc tgtccccgag ttgtggaagc agggaccaag
     3421 gaagaacgcc atgggccacc cgattcgatt cctcgggcgt caacgcgaca ctcgattttg
     3481 gtacaaaaca ggattccaag ggacggataa aaggcaggat atcttcctgg gcgggattct
     3541 cggatacctt ccaggcttct tcgagacgat acccgacaag gcttgtccgg cgcaatcccg
     3601 cgagacaggc tccgcaaccc agccggcttc ccatatcacg ggcaattgag cggatatagg
     3661 ttccggatga gcagatgatc tcgagagcaa cacggtcggt accccgttcc cgttcgttca
     3721 attcgaatac cgtcacactc cgctcgcgca gttcgggagc gatcccggcc cggaccagcg
     3781 cataggcccg ctttcccccg gtcttgaccg ctgagacagc cggaggaagc tggcggatct
     3841 ctccgcggaa ttcatcgtac agcgcaggca ggagttgttc caagccggcg ggaaccggat
     3901 ttcgggctgt aacactcccg tccagatcat cgctgtcggt cgtgacgccg aagagcacat
     3961 cggcacgata ggttttgggg aacgtatgaa accaggaaac gagccgggtt gcggcaccaa
     4021 aacagacgag gagaaggccg gatgctgccg ggtcaagggt gcccgcatgc ccgattcgca
     4081 ctcgccgaaa aacctttttg agaatccgca acacatcgaa ggatgtcatc ccgacaggct
     4141 tgtcaacggg aagcacggca gcccgaagaa agggcaaatc attctccggg tttgttggct
     4201 tcttcgataa tcctgtcaag tctcatgccc tcttcaatcg tccggtcaac atggaaacgc
     4261 aactcgggaa cccggcgcaa atccagaacc ccggagagtc tgctccgcaa aaagccggtg
     4321 acacgctcca gggccggaac aacctgtccg agggacgagg aatccagcac ggaaaaatac
     4381 acgtcagccg agccaagatc gcgggccatc cgtacctcat ggatcgtaaa aacggctccg
     4441 gctatcctgg ggtccttgac ttcctcatgc aggagtcgcg agatctcctg ctggacaatc
     4501 cgggccaccc gctcgggtct gtgcgagacc gccatgatcc ttacaccgtc tttccacccc
     4561 ggcccggggt aacttcctgc atgctgtagt tctcgaggat atcaccaacc cgaatgtcgt
     4621 cgtacccttc gacaccgata ccgcattcgt accccttttg aacctctcgg gcatcgtcct
     4681 tgaaccgctt gagagaagaa acctttcctt caaagatgac ttcaccctcg cggatgacac
     4741 gcaccttgtt gtttctgagg atggaacctt caatgacgta acaaccggcg atggttccgg
     4801 ccttgggaac cttgaaggta tcacgaacct cggcacttcc gctgaccttc tcgtgcaact
     4861 cgggtgtcat catcccgcgg agggcggaat cgatgtcatt gatggcatca tagatgattg
     4921 tgtacggccg gatctcgacg tgctcacgct ctgccatctc gcgagcccgg gtattgggcc
     4981 ggacgtggaa gccgatgatg atcgccttgg ttgttgccgc gagcatgaca tcatcttcgt
     5041 tgatcgcacc cgcagagtag cggatacatg tgagacgaac ctcgctgttc tcgattttct
     5101 cgagagaatc tttaagagcc tcggcagatc cctgtacatc agccttgatg atgaccttga
     5161 gttccttggt ttcaccggcc ttgatcttgt cgaagatgtt ttccatcgta acttttttga
     5221 cggtcttcgt gtcttcgaag cgcctgagtt caagccgctt gcccgaaatc gtccgtgccc
     5281 gtttttcgct ctcgacgata tggatcgggt caccagcctg gggaagtccg gaagatccga
     5341 ggatctcgac gggcatcgcc gggccgatct tttcgagttt ctcgccacga tcattgtaca
     5401 tggcacggat acgtccgtag tgcacacccg caacgaagtt gtcgccgacc gacatggatc
     5461 caccgagaca gagaaccgtg actgtcgttc ccttgccagg atcaattcgt gcttcgagaa
     5521 cggatcccat accgagcttg tcgggatcag ccgtcaactc gagcatctct gcctggagaa
     5581 gaaccgcttc gagaagctcg gggatgccct ttttctggat ggccgaaacc ggcacacaca
     5641 tggtcttgcc gccccagtcc tcgggaagga ggtcaagccg atccgccaac tgctgcttga
     5701 cgcgatcgat atttgcctca ggtttgtcca tcttgttaat ggcgaccacg atcgggaccc
     5761 cggcttcaat ggcgtgcttg gccgcctcga tcgtctgggg catcacgcca tcatcagccg
     5821 ccacaaccag gatcacgata tccgtgacct tggctccacg ggcacgcatg gtcgcaaagg
     5881 cctcgtgacc gggggtatcg atgaaggtga tcgcaccatg gggtgtatgc acctggtagg
     5941 caccgatatg ctgggtaatc ccgccagact cggtatccac aacattggcg gaacggattg
     6001 catccagaag tttggtctta ccgtggtcaa catggcccat cacggtaaca atcgggggtc
     6061 gggagacaat acgatccgga ctgtcgacga tctcgtcaat caccgtttca tcgtagaggg
     6121 atacctgttt gacggtacac ccgtactcgg aagcgacaag agtcgccgta tcggcatcga
     6181 tctgctggtt gatggtcgtc atcaatccca ttgtcatgag cttggaaatg atctccttgg
     6241 caggaagatt catctttcgc gccagttcac tgacagtgag cgtttccatg atctctattt
     6301 tttcggggac tgactcgagc tgtgtctccc gcttggtctt ctttttgaac gaagtcttgc
     6361 ggacaacatc ataatcatcg ttccagcgtt tgtccggcag acgatcgcgt ttttcttttt
     6421 ctttgccacg agtcacacca cctttatggg gctgacgtcg atcggaactg gccggaaatc
     6481 ccatgcccat cccgggcgca cccgatgatg cggccggtcg tcctcctccc gaggctggcc
     6541 gtcccgatcc gaagccgggt ctgccaccac cgccgccacc gtagggcggc cgctgcccac
     6601 caccaccgcc gccaccgtat ggtggtctct gacccccggc accaccaccg taaggcggtc
     6661 gttgaccacc agcatagcca ggtcggtctc cctgcccctg cgggcgactc ccgtagccag
     6721 gtcggtctcc ctgcgggcga ctcccgtagc caggtcggtc tccctgcggg cgactcccgt
     6781 agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaggtcg gtctccctgc
     6901 gggcgatccc cgtagccggg gcggtctccc tgcgggcgac ttccgtagcc ggggcggtct
     6961 ccctgcccct gcgggcgact cccgtacccg gggcgatcac cctgcgggcg acttccgtag
     7021 ccgggacggt ctccctgcgg gcgctgttct tgttgtatgg gtcgttcaac cggactcgcc
     7081 gattcagggg ctggtgtggt gaaatcagca accagctggg ctgtctccgg gagtcctgaa
     7141 ggccgtgccc cggaatcagc cgcctgttcc tcatcaccgg acgaggagcc ctgttccccc
     7201 tcgtgcccgc cagcggaggg tctttttttg atgacgatct ttttacgaac ctgggcaaca
     7261 gcggcatctt cagcctgttt tgtcttcttt atcagcatgg cctgcttgtg tgcgggctta
     7321 tggccatcgg tctggatcat cctctcgaca ctggcgaggg tatccgcagg gatcgggtct
     7381 gtcaccgcaa ggttttgcgc gcctgctctc tggacgtatt tatccacgtc cgtagcttgc
     7441 atattgtact tttttgaaag ctctgaaacc gtcatcaaca ttcccttcat cccgagctga
     7501 ttcttcagac acccaccagc atcgccacac attccatgcg gagtatccaa aatctacgga
     7561 ttttttgtcc aaaaacggac tatttcacgc gtcaaaaggc cagcacactc ccgcaattgg
     7621 gacaggtttt catgcccgag cgcaccttg
//
LOCUS       DAOSHE010000048         7571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8397_length_7571_cov_4.521022, whole genome shotgun sequence.
ACCESSION   DAOSHE010000048 DAOSHE010000000
VERSION     DAOSHE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8397_length_7571_cov_4.521022"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLD82_02395"
     CDS             <1..253
                     /locus_tag="PLD82_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64270.1"
                     /translation="RTLELLIEGGVQGMRFSLRDHGIPFDPGAAVPVDLDTHLASGNQ
                     DGLGIHMIRQLMDRMVWRRLPDGNELILEKDRIPGREGR"
     gene            complement(210..971)
                     /locus_tag="PLD82_02400"
     CDS             complement(210..971)
                     /locus_tag="PLD82_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64271.1"
                     /translation="MDEQQDLNFEYGDPLHPKGNALAYWKLTAHFGDESFEKLIVTNF
                     VISPMYIGKQSVAATFPPQLVDSLDDLVALAHRAEVDLIRVREVDLPAEEFDFESFFK
                     QQLSHFNRLVAQYTEQYGQALGTEDQPEIDAEPHDELTSLQNIIRLAEQARQAFLIQH
                     NAARARETLLKIRRMALALNSPTYKYDVEELLLLLNNPDSRIDDLSHLYFQKFMAICT
                     EQYEEAARLKQSIRRLAKRITVSDLPGRESGPSPE"
     gene            1104..2900
                     /locus_tag="PLD82_02405"
     CDS             1104..2900
                     /locus_tag="PLD82_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64272.1"
                     /translation="MEHDRQSIDAVRKRGGFDSGLDPHILSLDRAEQERLARGFRDAG
                     AAMESSADAAEGNPSERGAVPGKEAEGQPLGLWDRFVLFFLQLFTGITTEEFLKNREL
                     AHLAAHVRTFRPPLYNFRSNQLLGAFGETVYKLSTIMTGMRQLFEACRFTGPEGGGKG
                     FAEYFVRRMMPALPDLDSRYSQEYIRINRNLFGEKQIKATVERDVEQAAQGIGYEQRS
                     EINSLYANFVAFQRLAFFNYYALLRRFGENYAPGRGGFPEFSAAEGNDALFDLTRLEE
                     LLYSVDLSINMDSVFSALSGFADLLAGDGGGSASERPFWTEDDAARLRAAIMALLQDD
                     RLVSIVRLITRNPKRLPAVRRIPVNPVEEMKALQRARLVPRIQATLHQLEADELGKRI
                     SELFGTIELPEFEFYNETTNRRLQAMELPLFLHCRQVQIAKAFQQTMFDTMIRPALNS
                     IVMDGEFLDKGMQAALGDFFYRFNEVCGQVFEFERKVSLSTPEGDKVQNLILRFSGDP
                     PTRKVVTDKIHFLNNMAAKSLRDLAVLVVQAVRPLSAIVRDGGGQNKPEFVRNIRMVG
                     GARNKLVIKAVQRVLEVTNALAGILEPFAKEN"
     gene            3200..4672
                     /locus_tag="PLD82_02410"
     CDS             3200..4672
                     /locus_tag="PLD82_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOG64273.1"
                     /translation="MIRPKKSDFLAQARPGQRFAVVHEMPADRVTPVDAFYATGASYL
                     LESAERGMQIGRYSFLGISPDTKIEIRGTRCCVHAGAERREHEAADPLRIVGEYLRER
                     PYIAPGDLSPLPGGAVGYIGFDMVSRWERLRDIPVRPGLGLPDAMFLCTRYNLVFDNL
                     MHTLKIICNVETGEDLSADFDRAVDGIRDIAEQIEAAARGGLAGGGFSIGEIRSTFPR
                     ERFKNAVAHIQEMIASGEAIQVVLSQRMHAEFSGDSFAVYRALRSINPSPYMFYMDFG
                     DFQLVGASPEVMVRIQDDRVILRPIAGTRKRGADAAEDESLKAELLADPKERAEHMML
                     VDLARNDLGRIAKAGTVKVDRMMEVERYSHVMHIVSEVSAIPDPASDLFDIVRAVFPA
                     GTVSGAPKVRAMEILAQHEPVMRGPYAGMIGYFGYPGGLDSCITIRSLIVQDGKIYLQ
                     AGAGIVHDSVPEKEYEETLAKARAMFTALTKGAETGTDRH"
     gene            4688..6277
                     /gene="trpD"
                     /locus_tag="PLD82_02415"
     CDS             4688..6277
                     /gene="trpD"
                     /locus_tag="PLD82_02415"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG64274.1"
                     /translation="MVLIIDNYDSFTYNLVQYFMQLGQEVRVFRNDAITVAEAEEMDF
                     DYLVISPGPGGPEETGCSMDMLRRFVGVRPVLGVCLGHQTIGAFYGARIVRARKVMHG
                     KTDLIHHDGKSVYSGLPDPLRVMRYHSLAIERDSLPDCLEVTAQSDDGEIMGVRHREL
                     RVEGVQFHPESIGSEDGMRLLANFISGVREIPPVRTLLSRLAAGERLTREEAASLVGQ
                     TAEGRLSQAQIGAMLTAFSLRKPEVAEVSGIASALRARLPEFPLETERPVIDTCGTGG
                     DGSGSFNVSTAAAFVAVGAGAAVAKHGNRSITSRSGSADVLEVLGVPVDLSPDAARTS
                     IDRDGFAFLFAPHYHDAFKHIMGPRRELGFRTVFNMMGPLINPARVERQVVGVYSREL
                     VPLVAEVLGTLGLKHVMVVHGSDGMDEITLTGPTWVSEYRNGWVRHYEIHPAEYGLPL
                     CQAEAIRGGDAAANAGIINRVLEGERGPARDIVLLNAAAAILVADLASDYGEALVIAS
                     RSIDSGSALAALERARAGSLS"
     gene            6274..7191
                     /locus_tag="PLD82_02420"
     CDS             6274..7191
                     /locus_tag="PLD82_02420"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOG64275.1"
                     /translation="MKTILDEIVARRREDVAARKRTIRLPDVRTDAGYAAALADRPGG
                     GLRPLFRKPQGGPTNSPDMPARKGTEDVHQKKQQTGIIIAECKKASPSKGVMLPEYDP
                     VALALRYQAGGASAVSVLTEPEFFSGSDEDLRMVRRSTGLPVLRKDFMVDEWQIDESF
                     ALGADIVLLIASVLDVAELAGMIGRAHRLGMEVLLEARDGDELDTIRLSGLQPDAIGV
                     NSRDLRDFSVDPERLVKLTGAMPSGSVRVAESGIHATDTARMLFRAGFDAFLIGEHFA
                     LAADPEAEVRRFREVLDGEIQDGQGAGRE"
     gene            7188..>7571
                     /locus_tag="PLD82_02425"
     CDS             7188..>7571
                     /locus_tag="PLD82_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64276.1"
                     /translation="MKRIFVKICGLTDYDDAAFATEAGAEYLGFVLADSVRRIGLDDA
                     ATLIGRLRTCFGGEAPRMVAVVVNPSPEEMQTVSGIQCFDLVQVHHPAPELAAYRSVP
                     WYPVLPLAVGGAKGLERAAEQEYRFA"
BASE COUNT         1512 a         1879 c         2433 g         1747 t
ORIGIN      
        1 gcgcacactc gagcttctga tcgagggagg ggtgcaggga atgcgatttt ccctgcggga
       61 tcacgggatt ccgtttgatc ctggcgcggc tgtccccgtc gatctggata cacatctggc
      121 atcgggaaac caggacgggc tcggtattca catgatccgc cagctcatgg acaggatggt
      181 ctggcgaaga cttccggacg ggaatgaact cattctggag aaggaccgga ttcccggccg
      241 ggaaggtcgc tgaccgtaat ccgtttggcc agtctgcgga tggactgctt gagcctggcg
      301 gcttcctcgt attgttctgt gcagatggcc atgaatttct ggaagtagag gtgcgaaaga
      361 tcatcgatcc tgctgtccgg attgttcaac aacaagagca gttcctcgac atcgtacttg
      421 taggttggcg agttgagcgc aagggccatc ctccggattt tgagcaatgt ttcccgggcc
      481 cgggcagcgt tgtgctggat gaggaaggcc tgtcgtgcct gctcggccag gcggatgatg
      541 ttttgcaatg acgtgagttc atcatggggc tcggcgtcaa tctcgggctg gtcttctgtt
      601 cccagagcct gtccgtactg ttccgtgtat tgtgcgacca gacggttgaa atgcgagagt
      661 tgctgcttga aaaatgattc aaaatcaaac tcttcagccg gtaaatccac ctcacggacc
      721 cggatcagat cgacttcagc cctgtgggcc agtgcgacaa gatcgtcgag cgagtcaacc
      781 agttgtggcg ggaaggtggc cgcgacactt tgcttgccga tgtacatggg agagatgaca
      841 aaattggtca cgatcagttt ttcgaagctc tcgtccccga aatgtgcggt cagtttccag
      901 tatgccagcg catttccctt ggggtgtaac ggatctccgt attcgaagtt caggtcttgt
      961 tgttcatcca tggtgttccg tcctttctcc cgtaccatca gtataccaca gaatcacatt
     1021 gcgtgatgga ggggggcgtt gtagaatgcc ctatcccctc tgtatacagg gaaagatgcc
     1081 agaggcgagg gaacgtcacc ggtatggaac acgatcggca atccattgat gctgtgcgca
     1141 agcggggcgg ttttgattcc ggtcttgatc cgcatatcct ttcgctggac agagcagagc
     1201 aggagcgcct ggcacgggga tttcgtgatg ccggggctgc catggagagc agtgctgatg
     1261 ctgcagaagg caatccgtcg gagagggggg ctgtccccgg gaaggaagct gaaggacagc
     1321 cgcttgggct gtgggaccgg tttgtcctgt tttttctgca actctttacc ggcatcacga
     1381 cggaagagtt tctcaaaaat cgcgagctgg cgcatctggc ggcgcatgtt cgcacattcc
     1441 gtcctccgct gtacaatttc cggagcaacc agttgctcgg tgcttttgga gagactgtct
     1501 acaagctgtc gacgatcatg acgggaatgc gacagctctt tgaggcctgc aggtttaccg
     1561 ggccggaggg tgggggaaag gggtttgccg aatactttgt ccggcgcatg atgccggccc
     1621 tgccggatct ggacagccgc tattcgcagg agtatatcag gatcaaccgg aatctcttcg
     1681 gagaaaaaca gatcaaggca acggtggaga gggatgtcga acaggcggcc caagggatcg
     1741 ggtatgagca gcgaagcgag atcaacagtc tgtatgccaa ttttgttgcg ttccagcggt
     1801 tggcgttttt caattattat gccttgttgc ggcggtttgg cgagaattac gcaccgggca
     1861 gggggggctt tcccgagttt tcggctgctg aagggaatga tgccctgttc gatctgacca
     1921 ggctcgaaga gcttctctat tcggttgatc tttccatcaa catggattct gtgttttcgg
     1981 cactctccgg atttgccgat ctcttggccg gggatggagg cgggagtgcg tcggagagac
     2041 cgttctggac agaagatgat gctgcccgtc tgcgagcggc catcatggcg ttgctccagg
     2101 atgacagact ggtttcgatt gtccgtctga ttacccggaa cccgaaacgg ttgcctgccg
     2161 tcaggcgcat tccggtcaat cctgttgaag agatgaaggc tttgcaacgg gcgagacttg
     2221 tgccgcggat tcaggcaacc cttcaccaat tggaagcgga tgagttgggc aagcgtatct
     2281 cggaattgtt cgggaccatc gagctcccgg agttcgaatt ttacaacgaa acgaccaatc
     2341 gcagacttca ggccatggaa ctgccgctct ttcttcattg cagacaggtc cagatcgcca
     2401 aagcatttca acaaacgatg tttgatacca tgatccgacc tgccctgaat tcgatcgtga
     2461 tggacgggga gtttctcgac aagggaatgc aggcagcact cggagatttt ttttaccggt
     2521 tcaatgaagt ctgtggacag gtttttgaat tcgagcggaa ggtttcgctc tccaccccgg
     2581 agggagacaa ggtgcagaat ctgattctcc ggttttccgg ggatcccccg acacgcaagg
     2641 ttgtcaccga caaaatccat tttctgaaca atatggccgc caagtcgctt cgtgatcttg
     2701 ctgtcctggt ggtacaggcg gtccggcctt tgtctgccat cgtccgtgat ggcggagggc
     2761 agaataagcc ggagtttgtg cggaatatcc gcatggttgg gggggcgagg aacaagctgg
     2821 tgatcaaggc ggtccagcgg gtgcttgaag tcaccaatgc ccttgccgga atactcgaac
     2881 cctttgccaa ggagaactga tttttccgga aggcgtgctt cccgaagtag tgcttgacag
     2941 gggagtacgt tttcgttacc atgttactat acatggtaac agaagagggt ggttctgttg
     3001 aaacggattt ctggagaatg gtggcgcggc cctgatgtgg ccgcacggcg caggtaggaa
     3061 gcagggaaac tgctgatctt cctgaaggca ggccgcaggg atgcagggtg tcggaaatac
     3121 ggtacaccct ggtcgcaacg gtttgccttt ttttattcgg tctggagtcg aaaggagcgg
     3181 gagtcgcatg gagacactca tgatacgtcc aaaaaaatcg gattttctgg cgcaggcacg
     3241 accggggcag cggtttgccg tggtccatga aatgcctgcc gaccgggtca ctccggtgga
     3301 tgcgttttat gccaccggtg cttcgtatct gctggaatcc gcggagcggg ggatgcagat
     3361 cgggcggtat tccttcctcg ggatctcgcc ggatacaaaa atcgagatac gggggacccg
     3421 gtgctgtgtg catgcagggg ctgaacggag agagcacgag gccgcggatc ccttgcggat
     3481 tgtcggcgaa tatctccggg agcggcccta tattgcacct ggtgatctct ccccgctgcc
     3541 cggtggtgcg gtcggatata tcggattcga tatggtctct cggtgggagc gattgcgcga
     3601 tattccggta cgtcctgggc tgggcctgcc ggatgcgatg tttctctgta cacggtacaa
     3661 tcttgttttt gacaacctga tgcataccct gaaaatcatt tgtaatgtgg aaactggaga
     3721 agacttgtcg gctgattttg accgggctgt ggacggcatc cgggacattg ccgagcagat
     3781 tgaagcggcg gcccgaggtg gccttgccgg tggcgggttc tcgatcgggg aaattcgcag
     3841 tacgttcccg agggagcggt tcaagaatgc ggttgcacac atccaggaga tgattgcatc
     3901 cggtgaggcg attcaggtcg tcctctccca gcggatgcat gccgagttct cgggggattc
     3961 ctttgcggtg taccgggcgc tgcggtccat caacccctcg ccgtatatgt tctacatgga
     4021 tttcggcgac ttccagttgg tgggggcctc gccggaggtc atggttcgga tccaggatga
     4081 ccgtgtcatc ctccgcccga ttgcgggaac acgcaagcgc ggagcagatg cggcggaaga
     4141 tgaatcactc aaggcggaac ttctggccga tccgaaggag cgtgctgaac acatgatgct
     4201 tgttgatctg gcgcggaatg atcttggaag aatcgcgaag gccgggactg tcaaggtgga
     4261 caggatgatg gaggtggagc gctactccca tgtgatgcat attgtcagtg aggtttcggc
     4321 catacctgat cctgccagtg acctgtttga cattgtccgg gcggtcttcc cggccggaac
     4381 ggtttccggg gcgccgaaag tccgggcgat ggagatcctg gcacagcatg aaccggtcat
     4441 gagaggaccg tatgccggaa tgatcgggta tttcgggtat ccgggcgggc tggactcatg
     4501 catcaccata cgttcattga ttgtgcagga cggaaaaatc tatctccagg ccggggccgg
     4561 catcgtgcat gactctgtcc cggaaaaaga gtatgaagaa acactggcca aggcgagggc
     4621 aatgttcaca gcgctgacga agggtgccga gaccggcact gaccgacact gagacagggg
     4681 cgtcaccatg gtactgataa tcgataatta cgattcattc acatataatc tggtgcaata
     4741 tttcatgcag cttggccaag aggtgcgtgt tttcaggaat gatgccatca ccgtggccga
     4801 ggcggaggaa atggattttg actacctggt catttctcct ggtccgggcg ggcctgaaga
     4861 gacaggttgc tccatggata tgctgcgccg gtttgtcggg gtcagaccgg tcctcggtgt
     4921 ctgcctcggc caccagacga tcggggcgtt ttacggggcc cggatcgtgc gtgcccggaa
     4981 ggtcatgcac gggaaaaccg atttgatcca ccatgacggg aaatccgtct atagtggact
     5041 gccggatccc cttcgagtga tgcgctacca ttcgctggcc attgaacgcg acagccttcc
     5101 ggattgtctc gaggtcactg cccagagtga tgacggcgag atcatgggtg tccggcacag
     5161 ggagttgcgt gtggagggtg tccagtttca tcctgaatcc atcggcagtg aagacgggat
     5221 gcggcttctt gcgaatttca tcagtggcgt ccgggagatt ccgccggtcc ggacgcttct
     5281 ctctcgattg gcagccggtg agcggctcac cagggaggaa gcggcgtcgc tggtcggaca
     5341 aacggcagag ggccgtttgt cccaggccca gattggagcg atgctgacgg ctttttcact
     5401 gaggaagccg gaagttgcgg aagtcagcgg gattgcatcg gccctccggg ccaggctccc
     5461 ggaatttcct ctggaaaccg agcggccagt catcgataca tgcgggaccg gaggggacgg
     5521 aagcgggagt ttcaatgttt caaccgcagc ggcttttgtc gctgttggcg ccggtgccgc
     5581 ggttgccaaa cacgggaatc gctccatcac atcccgttcc ggctcggcgg atgtccttga
     5641 agtgcttggg gtgccggtcg atctttcacc ggatgcagcg cggaccagta ttgaccggga
     5701 cgggtttgcc tttctttttg ctccccatta ccatgacgcg ttcaaacata tcatgggtcc
     5761 gcgccgggaa ttggggttca ggactgtgtt caacatgatg ggaccattga tcaacccggc
     5821 acgggtggag cggcaggttg tcggagtcta ttccagggag cttgttcctt tggtggccga
     5881 agttcttggc acactgggct tgaagcatgt gatggttgtc catggatcgg acggaatgga
     5941 tgaaatcaca ttgacaggtc cgacctgggt cagtgaatac cggaatggct gggtcagaca
     6001 ctacgagatt catcctgcgg aatacggtct tcctctctgc caggccgaag cgattcgtgg
     6061 cggggatgcg gcggcgaatg ccggaatcat caatcgtgtc cttgaagggg aacggggtcc
     6121 ggccagggat atcgtcctgt tgaatgcagc agcagccatt ctcgttgcgg acctggcgtc
     6181 ggattatggt gaggctctgg tcattgcttc ccgttccatt gactcgggga gtgccctggc
     6241 ggcacttgag cgggccagag cggggtctct gtcatgaaaa caatcctgga cgagattgtg
     6301 gccagacggc gtgaggatgt cgctgcgaga aagcgcacga tccgtttgcc ggacgtcagg
     6361 acagacgccg ggtatgcagc cgctctggct gaccggcccg gaggggggct gcgcccgctc
     6421 ttcaggaaac cgcagggtgg tccgacaaat tctccggaca tgccggcccg caaggggact
     6481 gaggatgttc atcaaaaaaa acaacaaacc ggtatcatta ttgccgagtg caagaaagca
     6541 tcgccctcca agggggtcat gctgcccgag tacgatccgg tggctctggc gctgaggtat
     6601 caagccggtg gtgcgtcggc tgtttccgtt ttgacggaac cggaattttt ttccggatct
     6661 gatgaagacc tgcgcatggt ccgcaggtcg acaggattgc ccgttctccg caaggatttc
     6721 atggttgatg aatggcagat cgatgaaagt tttgctttgg gcgcggatat tgtcctcctg
     6781 atcgcatccg tgcttgatgt cgcggaattg gccggcatga tcggtcgcgc gcaccggcta
     6841 ggaatggaag ttttgctgga ggccagggat ggagacgagc tcgatacaat ccgtttgtcc
     6901 ggtctgcagc cggatgcgat tggtgtcaat tcgagagatc tcagggattt ctccgtcgat
     6961 ccggagcgtc tggtcaagtt gacaggggcg atgccgtcag ggtcggtccg ggttgccgaa
     7021 tcgggaatcc atgcaaccga tacggcccgt atgctctttc gtgccgggtt cgatgccttc
     7081 ctgatcggcg aacattttgc gttggcggcc gatcccgaag cggaagtcag gcggtttcgg
     7141 gaagtccttg atggcgagat tcaggacggg caaggggctg gccgggaatg aaacggatct
     7201 tcgtcaaaat ctgtggactg accgactacg atgacgcggc attcgcgacc gaggccgggg
     7261 ccgagtatct cgggtttgtg ttggcagatt ctgtacggag gatcgggttg gacgatgccg
     7321 caacgctgat tggccgcttg agaacttgct ttggaggaga ggcgccgagg atggtcgctg
     7381 tggtcgtcaa tccatcgccc gaagagatgc agactgtgag cgggatccag tgttttgatc
     7441 tggtccaggt ccatcatccc gctccggaat tggcggcata ccgatccgta ccctggtatc
     7501 cggttcttcc gttggctgtt ggtggggcaa agggcctgga gcgtgctgcg gaacaagaat
     7561 acaggttcgc a
//
LOCUS       DAOSHE010000049         7539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8448_length_7539_cov_3.870123, whole genome shotgun sequence.
ACCESSION   DAOSHE010000049 DAOSHE010000000
VERSION     DAOSHE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8448_length_7539_cov_3.870123"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLD82_02430"
     CDS             complement(<1..587)
                     /locus_tag="PLD82_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOG64277.1"
                     /translation="MPDTVPELTEAAQEAAHPLPVGGSLKDEYAGRQMSLNDLSQMTV
                     EKLAMLAQQFAVENISSKRKSDLIFSILKAHTDRSGQIYASGVLEVLQDGYGFLRSPI
                     YNYLPGQDDIYVSPSQIRHFGLKTGDTVSGQVRPPKEGERFFALLKVEQVNFEHPEVA
                     QRRILFDNLTPLYPMKKLRLETESAKVGMRIMDLFT"
     gene            complement(1096..1737)
                     /locus_tag="PLD82_02435"
     CDS             complement(1096..1737)
                     /locus_tag="PLD82_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG64278.1"
                     /translation="MALNEGMTSKFGVTYQPGQIIFLEYEPGDSFFLIQSGRVKITKV
                     VKDKEKLLDILEPGDVFGEMAILEDAPRSASAVAADEVKALLFRKENFEIILKSNPAM
                     ALKLLKIFAKRIYEQKRRLMIFTLEEDDAKVLDVLLMLAEQKGDNPNSIESVELVTNE
                     EQIASWAAIKVENCRKVLTHYDKLGRIQLGQNKVSIRNINEIHRMVATKRKQQ"
     gene            complement(1739..2779)
                     /locus_tag="PLD82_02440"
     CDS             complement(1739..2779)
                     /locus_tag="PLD82_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64279.1"
                     /translation="MSPVSRVYKAGSIIYFEGDKAETVFVLQAGVVLLTYISPEGTGE
                     VRENIKSGEFFGVKSALGRFPREETATVLSDATTMVMNVAEFEQLVLQNHKLVMKMLK
                     VFSNQLRRIGRSIQTRTGTADSEPSGTGLFNIGEYYLKNKKFNQALYAYQKYLQYYPD
                     GEYGTICLQRVEMAQSGAATGYAVASDGSSFVSQPVAKPAHEPVSEADTPAIDGDSGI
                     GIAKRYFDAFSLYSSEKYDEAYKLYKTILDSASGGEDEYVEKTYFDIGRCLFNMNKPQ
                     DAITQLTTMVKTFPTSPNLKAALFHIGKCYAMLGDTEKAKGFYNKVRMMPPNESINKR
                     AQKAIEELGGSV"
     gene            complement(3050..3559)
                     /locus_tag="PLD82_02445"
     CDS             complement(3050..3559)
                     /locus_tag="PLD82_02445"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOG64280.1"
                     /translation="MDYRSLIRDIPDYPKPGIVFKDLTGIWKDPVAHRASARDIADHF
                     RESGITKVAAAEARGFITGSLVAAELGAGFLPVRKPGKLPWETISESYELEYGTDTLE
                     MHVDAVTPGDRVLLVDDLVATGGTLAAIVRMIRRQGGEAVGVGTLVSLDFLKPGDKLD
                     IPVFALVRY"
     gene            complement(3665..4783)
                     /locus_tag="PLD82_02450"
     CDS             complement(3665..4783)
                     /locus_tag="PLD82_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG64281.1"
                     /translation="MSAMKQRPPQSAVMALISRAETRGHITQGEIDRAIERKLLKSED
                     IDWFLEELGTRGIRVDELAPKEETVDESPEIVGRDDPLSLYLAQVGKIPLLTRQQEHV
                     LFERIGMIQAELEKRPDDQDLVRRLGFARSIVIQGNLRLVISIAKRYQKLGLSLLDLV
                     EEGNIGLIEAVNRFEPARNVRFSTYGTWWIRQAIIKGLTEKARIIRIPVHIITRIKEI
                     STAASELTQELGREPTFAEISHRAGIGYSRLMDILHVTQEPGSLDVPVEHGNMLQLGD
                     VVEDERADQPVERMLLEGLNRTVSRVLDVLEPREREVIVLRFGLDGRPARTLKRTGEI
                     LGITRERVRQIQQRALLRIRSCSIAPELRDFFVDGDGQ"
     gene            complement(4838..5647)
                     /locus_tag="PLD82_02455"
     CDS             complement(4838..5647)
                     /locus_tag="PLD82_02455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64282.1"
                     /translation="MMLLLAALAGIFFSGQLLAGGYVVQPGDTLWSISRRTGVPVAVL
                     QRLNGLAEGSIRVGQRLALDAGTSPPRKETSQTRYRVKSGESLWSIAQRHGMTIEALR
                     SLNRLRSDVLHAGQELIVYPEASPRNTGEKREPAQVRPAPAGTGQPVFSWPMRGKIVE
                     RFGTKSTGLVQGISIAAGSENAVRSTAGGEVAYAGTLRGYGRMVIVRHGDGFHSIYAH
                     LSNITVSKGQRVNAGNVLGQAGAIPGSVPAGVHFQLYKKGRAVDPMSFLRP"
     gene            complement(5740..6024)
                     /locus_tag="PLD82_02460"
     CDS             complement(5740..6024)
                     /locus_tag="PLD82_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG64283.1"
                     /translation="MAIHLLMRRYTITGRVQGVGFRYHAAMKARAVGVTGYAHNDPDG
                     SVEVYAEGTVEQLDALHLFLAQGPSFARVDSIRFSEGTIRVRAHRDFSTG"
     gene            complement(6006..>7539)
                     /locus_tag="PLD82_02465"
     CDS             complement(6006..>7539)
                     /locus_tag="PLD82_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64284.1"
                     /translation="AAHILGKTVGFDLEPHTAQFSRVVLDSPGFFRWLALSGDRKSLR
                     GDLTQDEMLEPGIQDSIIEEVRSIVQKEYERAGITHLEDPALDTGSSREVQETIIRDL
                     IARGFWTIPCHTWGGVGLPGYIGYNHQDGYPLFEYLDAEGADQSEHAFGMLTPVVTAR
                     GLSVNTAPTQRTPSEPWQPGIEFLAGFYPAVSARFGFDFLRLDYVDHVFDSVLEGKDD
                     IPLADRMTPAVMRACIQRAREWQPSTGAMAERMGYDLPGYASAGFDLILGADVLRQID
                     IEFLDDMLRFQKEIAEANRQRRTPASIQYAIDTHDTGHPQINALPADSGRQALLLRIL
                     LAGLGSAGKGRRPMYEVMGNQDMTTGLYEANNQPVSLEWRNDREFFEQQEKLRRFLSG
                     LRPVLAVSEIRQVQIEAGWAFWCIDRMDGVRLRLCCLCRPETGSLDADARDIRLYPFT
                     QYWFDEACIEEYNIQTGALSFVPIEVDGTIVAGRMAEGAVRIFVITENSQGSAGIWQS
                     IS"
BASE COUNT         1689 a         2222 c         1923 g         1705 t
ORIGIN      
        1 gtaaagagat ccatgatccg cattccgacc ttggcgctct ccgtctcgag gcgaagcttt
       61 ttcatcggat agagcggggt caggttatcg aagaggatcc tgcgctgtgc gacctcaggg
      121 tgttcaaaat tgacctgctc gactttgagc agggcaaaaa aacgctcccc ctccttgggt
      181 ggtcgaacct ggcccgaaac ggtatccccg gtcttcaaac caaaatgacg gatctggctc
      241 ggagagacat agatatcgtc ctgtcccgga agataattgt agatcgggga ccgcaaaaaa
      301 ccgtatccgt cctggagcac ctcaagcacc ccggatgcat agatctggcc acttctgtcc
      361 gtatgcgcct tgagaattga aaatataaga tcagattttc ttttactcga gatattttcc
      421 actgcaaatt gctgggcaag cattgccagc ttctcgaccg tcatctggga gagatcattg
      481 agactcatct gtctcccggc atactcgtcc ttgagactac cgccgacagg aagaggatgg
      541 gctgcttcct gagccgcttc tgtcaattcg gggactgtgt ccggcatatc aagcaattcc
      601 tgaccggatg actgtggtct tccttttgct ccctgcgact tgccacgatg tggccgtcgt
      661 acggctacct gtcgggccgg tttagcagac tgtgtcggct gctgcggctg tgattcgggt
      721 tctctctgtg ttccatgtgc ttcttttgcg cgtccatcgc tgtcatcgcc gctttttttt
      781 cgcgacaccc tgactgccat atcttcctcc gtatgcatgt cgagcaaacc agatttctgg
      841 tctcgggtct gtgtatcggg attacgtcat gtggtaccat catggccggc tccgagtagg
      901 tcagaaagcc ggtccgcatc gcccggaaga aaccgggaat ctccgggccc ttattatctt
      961 aaccggacag aaaacaagca cacaaaacac cggagaggtt ctccgacgtc acagctcacc
     1021 gtatgcttgg ttgtatccaa aactcaggac tcatgcaatt ctgctacaaa aggagattca
     1081 agtcaataaa aaaaactatt gctgttttcg ctttgtcgcg accatgcgat gaatctcgtt
     1141 gatgttccgg atcgagacct tgttctgccc gagctggatc ctgcccagct tgtcataatg
     1201 cgtcaggacc ttgcggcagt tttcgacctt gatcgccgcc caagacgcga tctgctcctc
     1261 atttgtcaca agctccacgc tctcgatcga gttggggttg tccccctttt gctccgcaag
     1321 catgagaagg acatccagaa ccttggcatc atcctcttcg agagtgaaaa tcatcaaccg
     1381 ccgtttctgt tcataaatac gcttggcaaa gatcttgaga agcttgaggg ccatcgccgg
     1441 attcgacttt aaaatgatct cgaaattttc cttgcggaac agcagggcct tgacttcatc
     1501 ggccgcaacc gccgaagcac tgcgtggcgc atcttcgagg atggccatct caccaaagac
     1561 atcaccgggc tcgagaatgt cgagaagctt ttccttgtcc ttgacaacct tggtaatctt
     1621 gacccttccg gactgaatca gaaaaaaact gtcccccggt tcgtactcaa gaaagatgat
     1681 ctggccgggc tgataggtta caccgaactt gctggtcatc ccttcgttca acgccatctc
     1741 acacgctccc gcccagttct tcgatcgcct tttgcgcccg tttgttgata ctctcgttag
     1801 gcggcatcat ccgtacttta ttgtaaaagc ctttggcctt ttcggtatca ccgagcatgg
     1861 catagcattt tccgatatgg aagagtgctg ccttgagatt cggtgatgtc ggaaatgtct
     1921 tgaccatggt cgtcaactgg gtgatggcat cctgtggctt gttcatgttg aacagacagc
     1981 gaccgatgtc aaaataagtc ttttcgacat actcgtcttc cccgcctgat gccgaatcca
     2041 gaatcgtttt gtaaagtttg tacgcttcgt catacttttc ggaagaatac agggaaaaag
     2101 cgtcaaaata cctcttggcg atcccgatcc cgctgtcacc atcaatcgcg ggggtatccg
     2161 cctcggagac cggttcgtgg gccggtttgg caaccggctg ggaaacaaaa ctgcttccgt
     2221 cgctggcaac agcgtacccg gtggcggcac cactctgcgc catctcgaca cgttgaagac
     2281 agatcgtacc gtactctccg tccggataat actgcaggta tttctggtag gcgtacaggg
     2341 cctgattgaa cttcttgttt ttgaggtaat actcgccaat attgaacagc ccggttccgg
     2401 atggttcact gtccgctgtc cccgtccttg tctggatgct ccggccaatt ctccgcaact
     2461 ggtttgaaaa aaccttgagc atcttcatga cgagcttgtg attttgcaga accagttgct
     2521 cgaactcggc cacattcatg accattgttg ttgcatccga aagaaccgtt gccgtctcct
     2581 cacggggaaa ccgtccgaga gcggatttga caccgaagaa ctcgccgctc ttgatgtttt
     2641 cacgaacctc ccccgtaccc tccggagaga tatacgtcaa aaggacaaca cccgcctgca
     2701 ggacaaacac cgtttcggcc ttgtctcctt cgaagtagat gatggatccg gccttgtata
     2761 cccggctcac tggggacatt ggttcaactc ctcgctggcc cgtctcccgc caactctgcc
     2821 aagacagttc agggagacgc ttgaaaagta tcttacccta cctatcggca aaaatcaatc
     2881 cctcccctcc tgcattggac aaaaaaaaac caccgctaca tgacatgtat cggcggtctt
     2941 ttctgcccgt aaggcaactg agcatcaagt agcgtatcgg gaggagctac tgatcatctg
     3001 taaaaatgtc gtcacccggg aaaaaaattt gaatgaaaac gagagtcatt cagtaacgga
     3061 cgagtgcgaa tacgggaatg tcgagtttgt ctcccggctt gagaaaatcg agcgaaacaa
     3121 gggtcccaac ccccacagcc tcaccgccct gcctgcgaat catccggact atcgcagcca
     3181 gcgtcccgcc cgttgccacc aggtcgtcca caaggaggac acgatctccc ggagtgacag
     3241 catcgacatg catctcgagt gtgtccgtgc cgtactccag ctcataggac tcactgatcg
     3301 tctcccaggg gagtttcccc ggtttgcgga caggcaggaa cccggcgccc agctcggcgg
     3361 ccaccaagga tcctgttatg aacccccgcg cctccgcagc ggccaccttg gtgatgccag
     3421 attcccgaaa atggtctgca atatcccggg ccgacgcccg atgggcgaca ggatccttcc
     3481 aaattccggt gagatccttg aaaacgatgc ccggtttggg ataatcggga atgtcgcgta
     3541 tcagcgatct gtagtccata cctgccccct gcctgcaatc tcgtcttgcg tccggaacct
     3601 catgaaaagc ggacttgaaa atctagtcac gaatgaacaa aaatgtcacg ctggaaatcc
     3661 tgtctcactg accatcccca tcgacaaaaa aatctctcaa ctcgggagca atgctgcaac
     3721 tccggatccg gaggagcgca cgctgctgga tctggcgaac gcgttcacgg gtgattccaa
     3781 gaatctcccc ggtccgcttg agagtccggg ccggtctccc gtcaagtcca aaacgcagga
     3841 cgatcacttc ccgctcacgg ggttcaagta catcaagcac cctggatacc gttcgattca
     3901 acccctcgag gagcatccgc tcaacaggtt gatcggcccg ttcgtcttcg acaacatccc
     3961 ccaattggag catgttgcca tgctcgaccg gaacatcaag cgaaccgggt tcctgtgtta
     4021 catgcagaat atccatcagc ctggagtacc cgatgcctgc ccgatgcgag atctcggcaa
     4081 aggtcggctc cctcccgagt tcctgtgtca attcggaagc ggcggtgctg atctccttga
     4141 tccgtgtgat gatgtggacc ggaatccgga taatccgggc cttttctgtc aaacccttga
     4201 tgatggcctg cctgatccac catgttccgt aggtcgagaa ccggacattt ctggcgggtt
     4261 caaaccggtt cacagcttcg atcagtccga tattcccttc ctcgacaaga tccagcaggc
     4321 tcaagccaag tttttggtag cgtttggcaa tactgatgac cagccgcaga ttcccctgga
     4381 tcacaatact gcgggcaaaa ccgagtcgcc tgacgagatc ctgatcatcg ggtctttttt
     4441 ccagttcggc ctggatcatc ccgatccgtt cgaagagaac atgttcctgc tgtcgtgtca
     4501 gcaaggggat ctttcccact tgggccagat atagactgag gggatcatcg cggcctacaa
     4561 tctcgggaga ttcatcgacc gtttcttcct ttggggcaag ttcgtcaaca cgaatccccc
     4621 tggttcccaa ttcctccaga aaccagtcga tatcctcgct cttcagaagt ttgcgttcaa
     4681 ttgccctgtc gatttcaccc tgggtgatat gcccacgtgt ttcggcccgc gagatcaagg
     4741 ccatgacggc actttgcggg ggacgctgtt tcatcgcgga catacggcac cccccatcag
     4801 tttttcagtc ccatgatggc acaggtgacc cccgaggtca aggccgcaga aaagacatgg
     4861 gatccacggc ccgacccttt ttgtacaatt gaaaatggac tccggccggg accgaacccg
     4921 gaatggcccc agcctgaccg agcacgttcc ccgcattcac cctctgcccc ttggaaacag
     4981 tgatatttga aagatgcgca taaatggaat ggaacccgtc cccgtgacgg acaatgacca
     5041 tccggccata acctcgaagt gttcctgcat aggctacctc gccaccagca gtactgcgga
     5101 cagcgttttc cgaaccggca gcgatcgaaa tcccctggac caatcccgtt gacttggtcc
     5161 cgaagcgctc gacgatcttg ccccgcatcg gccaggaaaa aaccggttgc ccggttcctg
     5221 caggcgccgg cctgacctgc gcaggttccc gcttttcccc ggtattccgc gggcttgcct
     5281 ccggataaac gatcaattcc tgcccggcat gcagaacatc ggagcggaga cggttcaggg
     5341 acctgagcgc ctcaatagtc atcccatgac gttgggcaat actccacagg ctctcgccgc
     5401 tcttgacacg atatctggtt tgcgaagtct cctttcgggg aggactggtc ccggcatcga
     5461 gagccagtct ctgcccgacg cggatgctcc cttcggccaa gccgttcaag cgctggagga
     5521 cggcaaccgg aaccccggtt cttctcgaaa tggaccagag cgtatctccg ggttggacga
     5581 catacccccc ggccagaagt tgaccggaaa agaatatccc tgccagggct gcaagaagca
     5641 gcatcatcca tgatctgtat gcggagtcca ttccaaattc agtatcgacg ggcaccatgc
     5701 tgaaactgaa ccggtcatag gtgtttttcg gaaaaggact cagcctgtgg aaaaatcccg
     5761 gtgggcccgg acccggattg ttccctcact gaaacggata ctgtccacgc gggcaaacga
     5821 tggtccctgg gcgagaaaga gatgcagtgc atcgagctgc tcgaccgtcc cttcggcata
     5881 gacttcgaca ctcccgtcag gatcgttgtg cgcatacccg gtcacaccga ccgctctggc
     5941 cttcatcgcc gcatgatacc ggaaaccgac accctgaaca cggccggtga tcgtgtagcg
     6001 acgcatcaag agatggattg ccatatccct gcacttccct ggctgttctc cgttatgaca
     6061 aagatgcgga cagcaccttc tgccatccgt ccggcgacaa tggtcccgtc gacttcaatc
     6121 ggcacgaacg acaaagcccc agtctgaata ttgtactcct caatacaggc ctcatcgaac
     6181 cagtattgcg tgaagggata gagcctgata tcccgtgcgt ctgcatccag actccccgtt
     6241 tcaggcctgc agagacaaca caagcgcagt ctgaccccat ccatccggtc gatgcaccag
     6301 aaggcccagc cggcttcaat ctgaacctga cggatttcgg aaaccgcaag aaccggacga
     6361 agtccggaca gaaaacgcct gagcttttcc tgttgttcaa aaaactcgcg atcatttctc
     6421 cactcgaggg acaccggttg attgttggcc tcatagagcc cggtcgtcat atcctgattg
     6481 cccatgactt cgtacatcgg ccgtctgcct ttaccggccg atccgagccc ggccaggagg
     6541 atccgcaaca agagggcctg ccttcccgaa tcggccggga gggcgttgat ctggggatgg
     6601 ccggtatcat gggtatcaat cgcatattgg atggatgcgg gagtcctgcg ttgacggttg
     6661 gcctccgcaa tctccttctg gaaacggagc atatcgtcaa gaaattcgat atcgatctga
     6721 cgcaggacat ctgctccgag aatcagatca aaaccggcag aggcatatcc ggggagatca
     6781 taccccatcc gctcggccat ggctccggtt gacggctgcc actcccgggc acgttgaatg
     6841 cacgcacgca tgacggcggg cgtcatccgg tcagccaggg gaatgtcatc cttcccctca
     6901 agaaccgaat caaagacatg gtcgacataa tcgaggcgca ggaagtcgaa gccgaatcgg
     6961 gccgaaacgg caggatagaa cccggccaga aattcgatac ccggttgcca tggttccgag
     7021 ggagtcctct gtgtcggagc cgtattcaca ctcaatccgc gagccgtgac aaccggagtg
     7081 agcatcccga aggcgtgttc tgactggtcc gcaccctccg catcaaggta ttcgaacagt
     7141 ggatatccgt cctgatggtt gtacccgata tacccgggga gtccgacccc gccccaggta
     7201 tggcagggaa tggtccagaa ccctcgggcg atcagatcac ggatgattgt ttcctgaacc
     7261 tcccgtgacg agccggtatc gagtgccgga tcctcaagat gggtgatccc ggcacgttcg
     7321 tactctttct ggacgattga ccggacttct tcgatgatgg agtcctgaat cccgggttcc
     7381 agcatctcgt cctgtgtcag gtcgccacgc aaggatttcc gatctccgga gagtgccaac
     7441 cagcggaaaa aaccggggga atcaagcacg acccgggaga actgggctgt gtgcggttca
     7501 agatcaaacc cgaccgtctt gcccagaatg tgcgccgca
//
LOCUS       DAOSHE010000050         7478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8533_length_7478_cov_4.175805, whole genome shotgun sequence.
ACCESSION   DAOSHE010000050 DAOSHE010000000
VERSION     DAOSHE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_7478_cov_4.175805"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLD82_02470"
     CDS             complement(<1..303)
                     /locus_tag="PLD82_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64285.1"
                     /translation="MILLSSTATPAVPDIDLSLGSFPIYILLFVFAWAGVLFIRRSLK
                     HRMPGQHIPSLVVSLASLVGAVLYGVLLGGLHVFTGAFSWTFLLAAFIFSFLVQTFK"
     gene            complement(452..>7478)
                     /locus_tag="PLD82_02475"
     CDS             complement(452..>7478)
                     /locus_tag="PLD82_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64286.1"
                     /translation="KNVAPDHTKALPLYTAALDAGTLPPDLDFRIRVSLAKSRLALSG
                     HANLEDARKDLEGLLKKTPAPSNVHKAELRYLLGRVFHSLGRRDSAEHYLTIAARSTN
                     TGEIPLLAASDLLNVLGFPSVNRRELARGRLVVRDVTTRYPGDKRSEALLFTTARAEI
                     HAGEREVGIASLEQCITRWPDGEHAAEALFRSAEALFSLNRHADAMQSLRTLLARFPG
                     SSFWQQAQRLMVDVEFGEAERATSGGEYVRAAELWKGFMARYPLDARTPTAALRLGHT
                     FEKRKDWKVAVDSLQDVWRRWPSTEQGQEACLEAGIILADRLDQYEAAIDLLKKPTIW
                     KFKQRAEQKIRELENESLALSTSGVFPEGTPPHVTLTVRNIAKVRVRIYQLDSEAYFR
                     SKAGFTGMEKLDIELISPDLDFWYMVPDYKKHKLGNVKIPVSLTGPGTWVIQCSTEKL
                     QSSTLLVKSDLGLTVRATRGQVVVQTTHLATGAAVPGATILVSNGKDVFATGTTDGDG
                     IFVSSATNEQAASGISVYARSGKNVAAVSPGWFDRYQPAKPRLVTHLAADRQAWRPGD
                     TVHFRLTLRIRDAAGLLSIPREEYLLTVRTPTGLEVMRNTVTPDRLGTIQGEFVIDPA
                     EAPGVISLVLVNADRTQTATHQLRVEQYTPKETSVEFTQSPTVPLVGDRISLTARCAH
                     LWGEPAAGRHILWRVNQEDWQTAVTDPRGEIIIPVDSAERISGQALTVTVKPSWEERV
                     YTHTINFASRDIALKVTPGRSIWLHTEKPVVEVTLAPIPGADPARKVSLTASLLTNGR
                     SLDMHSQSLTFAGKKPFTLAALRAGNYQITARTTSSSGEEVTARTNIFVSGPQDPRRI
                     HILQDTTEVSSGQALKLRIHSRLPRTQDGILLLERDRIVRQQKIRILPGESVITLFPD
                     EALSPAFKAVVLAATTNGFYTSEAEYALKHAIQLAITPPAAATPGKKVTLRFSTKSRS
                     GEAANSSLFVFVIDDAIWSAVNVSRQDIVSTFFPKLPFATTRSASFTPFTYSAKTGER
                     DPDMVSLERDRAAGRADMPSQRTANGNDRAFRQTREERPLDESENKGPGEPRHPAAWA
                     ELAWYSGSLTTGRDGTAEANFVLPDRSASWRVVTVAVSGATLFGDAELVMHAVTTTHA
                     RIQAPEAITSGDRVLVPLHLSTRSDKALQGRLTWQLETTNQQRITGGEIPWSLKPASA
                     SELSIPLTVSTPGSFILTVRDPAFGASVPLVVHPAGEKRVSGASALVRTVWEKRLRHT
                     PAAISRTLRIQAEHPEAILLEPYPEYFGRNQLSGIIASLVRDTAALEYLKAISPSDAL
                     RQNTLRASIESQARHLATRNKRGWESGAPTESEIAWLGFALQRCRAAGVTVDPALSAW
                     TKQRLEQIFSASGENEIKALTLFAMAGGSDSPFAWMNRLYRERARLAPRELALLTLAL
                     ESAGRRDMSGELVRLLQQAFIRKDGQAHIPGKPSRHTWWMEGSTGTTALAALALTAAT
                     SDQSSRTIIGEALAWLDEHRFGRVWSTPAENGIIPLVHARHLIAQASSAAWTAKVFVN
                     GREAGEFSSGRPGSITLTGDQLGGDGTTIKVQVEGRGACRVHSLLSEVLAPDAPPQRD
                     YPGTFEVSLQHPPYAIEGKSIPRGMSILADWTASPTNELARISRGSPFTAEIKVRMGG
                     TRVDELLVVELPVPTGCRTTGLIEGSAVIHRELLLGRVRYLVKAPHALETIRIREGMV
                     AFAEGSTRMTAASCAPLSAPWKRSTTAQRAFVIIPAGSDIFAGYRFSADEMYRIGEWH
                     FNRGEMDRAKALLSECLSRWQLKAGPAREIGLMLLDIAVREKNSREIVRHFESLKEKH
                     QDLSISLEQADAVAGAYARIGEHERSCQVLDGIIEARFLQDSAVSAAWLASNQVKEAA
                     ASLKKLWLEYPDGSEEIASISSFAQQLFQMATELSPEELAKRGVTGDWLKDESLTLFD
                     AVLAAAPDSTLAPESAFSVVSLLFEREHWQAAEARARKALSVYTNSHFVDSFRYLLAS
                     TLYFERKFAEASRECETVANTHFVQQDGSRGPSRYRDYALHMLGKIRHSEQRLADALK
                     LYEQVKARFADAARAIEHIRQKTLSLPEITIVPLEEQAHLEIASRNVSSATLTLYKVD
                     FLMLCLKQKDLRNVKGINLAGIRPSLTTNIALGKAGQVMTATARIPIPVRDTGAWLVV
                     VKADGIEQSGMIIRSNLVLDVMTTSDGSVRASLHDRKTTLPMIGAQLKFIPANGQRAT
                     TATSDPRGMAESQAVQGSMIVAAVHQGQYAFQRIESRVVASGNTDQDYGTDYKDRSME
                     GLKQYRRSIQDTNRARWEQNQKLEKRKLELKNLKF"
BASE COUNT         1352 a         2085 c         2480 g         1561 t
ORIGIN      
        1 tttgaatgtc tggacgagaa aagaaaaaat gaaggcggcc aagagaaagg tccagctgaa
       61 ggctccggta aagacatgca ggccgccaag caagactcca tagaggacag ctccgacaag
      121 ggaggccagg gaaacaacga gcgaaggaat gtgctgcccc ggcatgcggt gcttcagaga
      181 acggcggatg aagaggactc cggcccaggc gaagacgaag agcaggatgt agatcggaaa
      241 gcttcccaga gagaggtcaa tatccggaac cgccggagtc gcggtggatg aaagcaggat
      301 caagagagag tcccccctgt atcagtgtgc atccagtata ccccgggtaa acctgaaaga
      361 aaagcagctt gtcgtttgat ccggatcgtc gcgaaaaaaa ggggctgccc tttccggaca
      421 gccccctctt gtctcacaag attttttcgg ttcagaattt gaggtttttg agttccagtt
      481 tccgtttttc aagcttctgg ttctgttccc agcgcgcccg gttagtgtcc tggatcgatc
      541 tccggtattg cttcagcccc tccatggatc tgtccttgta atccgtacca tagtcctgat
      601 ccgtattccc gctggctacg acacggctct cgatccgctg gaaggcgtat tgcccctggt
      661 ggaccgccgc gacgatcatg cttccctgga cggcctgtga ctctgccatc cctcggggat
      721 cgcttgtggc tgtcgtcgcc cgctgaccgt ttgccggaat gaacttgagc tgggcaccga
      781 tcatggggag tgtcgtcttt ctgtcgtgca gggatgcacg gacgcttccg tcacttgttg
      841 tcatcacatc cagcacgaga ttgctccgga tgatcatgcc cgattgttcg atcccgtccg
      901 ccttgacgac gaccagccag gctccggtat cccggaccgg aatcgggatc cgtgctgtcg
      961 cggtcatgac ctgtcccgct tttcccaagg cgatattcgt ggtgagactg ggtctgatgc
     1021 cggccagatt gattcccttg acgtttctca gatccttctg tttgagacag agcatgagga
     1081 agtcgacttt gtagagagtc agggtggcac ttgacacatt ccgggatgcg atttcgagat
     1141 gcgcctgttc ttcaaggggg acaatcgtga tttcgggcag ggaaagagtt ttttggcgga
     1201 tgtgttcgat tgccctggcc gcgtccgcaa agcgtgcctt gacctgttca tagagcttga
     1261 gggcatcggc gagacgctgt tctgagtggc ggatcttgcc aagcatgtgc agggcataat
     1321 cccggtaccg gctcggtccc cggcttccgt cctgctggac gaagtgggta ttcgcgacag
     1381 tctcgcactc ccggctggcc tcggcgaatt tgcgttcgaa atacagcgtg ctcgccagga
     1441 ggtagcggaa ggaatcgaca aagtgcgaat tcgtatagac ggagagcgcc ttgcgggcac
     1501 gcgcctctgc agcctgccaa tgttcgcgtt cgaagaggag cgagacgacc gagaaggcac
     1561 tctccggtgc gagcgtggag tccggcgcgg ctgcgaggac tgcgtcaaag agggtgagcg
     1621 actcgtcctt gagccagtcc ccggtcacgc cacgcttggc aagttcttcg ggagagagct
     1681 cggttgccat ctggaagagc tgttgtgcga aggaggaaat actcgcgatc tcttccgacc
     1741 cgtccgggta ttccagccag agttttttga gggaggcggc ggcctctttg acctgattgg
     1801 atgccaacca ggcagcactg acggccgagt cctggaggaa tctcgcttca atgatcccgt
     1861 ccagaacctg gcaggaacgc tcgtgttcgc cgatccgtgc ataggctccg gcaacggcat
     1921 cggcctgctc cagcgaaatc gagaggtcct ggtgcttctc cttgaggctc tcgaaatgcc
     1981 ggacgatctc cctggaattc ttctcgcgga cagcgatatc gaggagcatc agcccgatct
     2041 ccctggccgg accggctttg agttgccagc gggagagaca ctcggagagg agagccttgg
     2101 cccggtccat ctcgccacgg ttgaagtgcc actctccgat ccggtacatc tcgtcagctg
     2161 agaaacggta gcctgcaaag atgtcgctgc cggcagggat gatgacaaac gctctctggg
     2221 ctgtcgtgct gcgcttccag ggtgccgaga gtggtgcgca ggatgcggct gtcatgcgcg
     2281 tactgccctc ggcgaaagcg accatgcctt cacggattcg tatcgtctcc agcgcatgtg
     2341 gagccttgac cagatagcgg actctcccca ggaggagttc gcggtggatg acggcagaac
     2401 cctcgatcaa gcccgttgtc ctgcatccgg tcggaacggg aagttcgacg acaagcaatt
     2461 cgtccacccg ggttcccccc atccggacct tgatctctgc cgtgaaagga cttccccggc
     2521 tgatccgggc cagttcgttt gtcggactgg cggtccagtc ggcgagaatc gacatcccgc
     2581 ggggaatgga ttttccctcg atggcatagg gggggtgctg gagagaaacc tcgaaggtcc
     2641 cgggatagtc ccgctggggt ggtgcgtccg gtgcgaggac ctcgctcagg aggctgtgga
     2701 cacggcaggc cccccgccct tcgacctgga ccttgatcgt cgttccatcc cctccaagct
     2761 ggtcaccggt cagggtgatg gatcccggcc ggccactgga gaattcgccg gcttcacggc
     2821 cgttcacaaa aactttcgca gtccaggcgg cggacgaagc ctgtgctatc agatggcgcg
     2881 catggaccag ggggatgatc ccgttttcgg ccggggtgct ccagactcgt ccaaagcgat
     2941 gctcgtccag ccaggccagc gcttcaccga tgatggtgcg gctgctctgg tcactggtcg
     3001 ctgctgtcag tgccagggcg gccagggctg ttgtgccggt ggatccctcc atccaccagg
     3061 tgtggcgtga aggtttgccc gggatgtggg cctggccgtc tttgcggatg aaggcctgct
     3121 gcaggagccg gaccagttcc ccgctcatgt cacggcggcc ggcgctctca agggcgagcg
     3181 tgagaagggc cagttcccgc ggggcgaggc gtgcccgttc acgatagagc cggttcatcc
     3241 atgcgaacgg ggaatcgctg ccacccgcca tggcaaagag tgtcagtgcc ttgatctcgt
     3301 tctctcccga agcactgaag atctgctcaa gccgctgttt ggtccaggcg gagagagccg
     3361 ggtcaaccgt gacccctgcg gccctgcaac gctggagggc aaagccgagc caggcgatct
     3421 cgctttcggt cggtgcaccg gactcccagc cgcgtttgtt ccgggtcgca agatgtctgg
     3481 cctgcgattc gatggaagcc cggagtgtgt tctgccggag ggcatcggag ggggagatgg
     3541 ctttgaggta ttccagggct gccgtatccc ggaccagact ggcgatgatg ccggagagct
     3601 ggtttctccc gaaatactcg gggtatggct cgagaaggat cgcttccggg tgttccgcct
     3661 ggatgcgcag ggtgcgggag atcgcggccg gagtatgacg gagtcgtttc tcccagactg
     3721 tgcggaccag ggccgaggca ccgctgacac gtttttcacc cgccggatgg acaaccaagg
     3781 gtacactggc gccaaaggcc ggatcccgaa ccgtcaggat gaaggatccc ggggtggaaa
     3841 cggtcagggg gatggagagt tcgctggccg aggccggctt gagtgaccag ggaatctctc
     3901 ctcccgtgat gcgttgctga tttgttgttt cgagttgcca ggtcaaccgg ccctgcaaag
     3961 ctttgtcgga acgggtcgag aggtgcaggg ggacaagaac cctgtctcca ctggtgatcg
     4021 cttccggagc ctggatgcgg gcgtgggttg tcgtgacggc gtgcatcacc agttcggcat
     4081 caccgaagag cgtggctccg gagactgcaa cagtgacaac acgccagctg gctgaccggt
     4141 ccgggagaac aaagtttgcc tcggccgttc cgtcccgacc tgtcgtcaga cttcccgagt
     4201 accaggccag ttccgcccaa gcggcggggt gtctgggctc tcccggacct ttgttttcgc
     4261 tctcgtccag cggtcgttct tctctcgtct gacggaaggc acggtcattg ccgttggcgg
     4321 tccgctggga cggcatatcg gccctgccgg cggccctgtc ccgttcaagg ctgaccatgt
     4381 ccgggtctct ttcgcccgtt tttgccgaat aggtgaaggg ggtgaaggag gcggagcgcg
     4441 tggtggcaaa cggcagtttg gggaagaagg tgctgacgat gtcctgtcgc gagacgttga
     4501 ccgcggacca gatggcatcg tcaatcacga aaacgaaaag gctggagttg gctgcttctc
     4561 cggaccggga ttttgtggag aagcggaggg taaccttctt gccgggggtt gctgccgcag
     4621 gtggggtgat ggcgagctgg atggcatgtt tgagggcgta ttcagcctcg gaggtgtaaa
     4681 acccgttggt ggtggcggcc agtacgaccg ctttgaaggc cggagagagt gcctcatccg
     4741 ggaagagtgt gatcaccgat tcacccggga gaatccggat cttttgctgg cggacaatcc
     4801 tgtcccgctc aaggagcagg atgccgtcct gggtccgcgg gagccgggaa tggatcctga
     4861 gcttgagtgc ctggccactg gagacctcgg ttgtgtcctg gaggatgtgg atccggcggg
     4921 gatcctgtgg tccactgaca aagatgtttg tcctggctgt gacctcttcc cccgaggagg
     4981 acgtggtccg cgccgtgatc tggtagttgc cggcacggag ggctgccagg gtgaacggtt
     5041 ttttccctgc aaaggtcaaa gattgggagt gcatgtccaa gcttcttccg ttcgtgagga
     5101 ggctggcggt cagactcacc ttgcgggccg gatcagcccc gggaatgggc gcaagcgtca
     5161 cttcgacaac cggtttttcg gtgtgcagcc agatcgatct cccgggagtc actttcagag
     5221 cgatgtccct gctggcgaaa ttgatggtgt gtgtgtagac acgctcttcc caggacggtt
     5281 tgacagtcac ggtcagggcc tgcccgctga tccgttccgc cgaatcgacc gggatgatga
     5341 tctcgccacg ggggtcggtg acagcggtct gccagtcctc ctggttgaca cgccagagga
     5401 tgtgccgtcc ggccgctggc tcgccccaga gatgggcgca gcgtgcggtc aggctgatcc
     5461 ggtcgccgac cagcggaacc gtcggggact gcgtaaattc gacggatgtt tctttcgggg
     5521 tgtattgctc cacccggagc tggtgcgtgg cagtctgggt acgatccgca ttgacgagaa
     5581 cgaggctgat cactccgggg gcctcggccg gatcgatgac gaattctccc tggatggtgc
     5641 cgagtctgtc gggcgtgacg gtgttcctca taacctcaag accggtggga gtgcggaccg
     5701 tcaggagata ctcttcgcgt ggtatcgaga gcagtccggc tgcatccctg atacggagcg
     5761 tgagccggaa gtggacagtg tcgcccggac gccaggcctg tcggtcggct gcgagatggg
     5821 tgaccaggcg gggttttgcc ggctggtacc ggtcgaacca gcccggggaa accgcagcga
     5881 catttttccc ggaccgggcg tagaccgaaa taccagaggc ggcctgttcg ttggtcgcac
     5941 tggaaacaaa gattccgtcc ccgtcggtgg tcccggtcgc aaagacgtcc ttgccgttgg
     6001 agacgaggat ggtcgctccg gggaccgcag ctcccgttgc gagatgggtg gtctggacga
     6061 cgacttggcc acgggtggcc cggactgtca gaccgagatc gctcttgacg aggagtgtgg
     6121 aagactggag cttttcggtg gaacactgga tgacccaagt acccggtcct gtcagactga
     6181 ccgggatctt cacgtttccg agcttgtgtt ttttgtagtc gggaaccatg taccagaaat
     6241 ccagatcggg gctgatcagc tcgatatcca gcttttccat accggtaaaa ccggccttgc
     6301 tgcggaaata ggcctcgctg tcgagctgat agatgcggac acggaccttg gcgatattgc
     6361 gcaccgtgag agtgacatgg gggggcgtcc cttcgggaaa gactccggac gtggagagtg
     6421 ccagggactc attctccaac tcgcggatct tctgctcggc acgttgcttg aatttccaga
     6481 tcgtcggttt cttcaggagg tcgatggcgg cttcgtactg gtcgagtctg tccgcgagga
     6541 tgatgccggc ctcgagacag gcttcctgtc cttgttcggt tgagggccaa cgtcgccaga
     6601 catcctgcag tgaatcgacg gcaaccttcc agtccttgcg tttttcgaag gtatggccga
     6661 gacggagggc ggccgtcggg gtgcgggcat ccagcgggta gcgtgccatg aatcccttcc
     6721 agagttctgc cgcgcggacg tattcgccgc cgctggtggc ccgctcggcc tcgccgaact
     6781 cgacatccac catcaatcgc tgcgcctgtt gccagaagct gcttcccggg aagcgcgcca
     6841 gaagggtacg cagggattgc atggcatccg catgccggtt gagggagaag agggcttctg
     6901 cggaacggaa gagggcttcc gcggcatgtt ctccatcggg ccagcgggtg atgcattgtt
     6961 caagagacgc gatgcccacc tccctctcgc cggcatggat ttcggcccgg gcggttgtaa
     7021 agagcagggc ctcgctgcgt ttgtctccgg gatagcgtgt ggttacatca cggacgacga
     7081 gccggccgcg cgccagttcc cggcggttga ccgaagggaa cccgaggaca ttgaggaggt
     7141 ccgaagccgc gaggaggggg atctcccccg tgtttgtgct ccgggcagcg atggtcagat
     7201 agtgctcggc actgtcccgg cgaccgagac tgtggaagac ccgtcccaaa agatagcgca
     7261 actcggcctt gtgcacattg gagggtgcgg gtgtcttttt gaggagcccc tccagatcct
     7321 tgcgcgcatc ctcgagattg gcatgtccgg agagtgcgag tcttgatttt gccagcgaga
     7381 cgcggatcct gaaatcaaga tcgggcggga gggttccggc atcgagtgcc gcggtgtaga
     7441 gcgggagggc cttggtatgg tccggtgcga cattcttg
//
LOCUS       DAOSHE010000051         7470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8544_length_7470_cov_4.753068, whole genome shotgun sequence.
ACCESSION   DAOSHE010000051 DAOSHE010000000
VERSION     DAOSHE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8544_length_7470_cov_4.753068"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLD82_02480"
     CDS             complement(<1..322)
                     /locus_tag="PLD82_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64287.1"
                     /translation="MSGPVKDTEDHCFRNRVGESRFYDRNQSADQVCGAASGVSGRKC
                     EAVNVVREVLHKTGHGSEGAETLSDADARLERLMLGLRTREGTSLADVGDAAALARFA
                     GGGFV"
     gene            446..1213
                     /locus_tag="PLD82_02485"
     CDS             446..1213
                     /locus_tag="PLD82_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64288.1"
                     /translation="MESIIGLIEGFSTLPASDIKDIFSAHLAPALEGHIRILLSQNLH
                     APAALPWLKASVLISRARKNSDKTGFTKATLLLLDALDKLDQPPLLDKSTTLHLYNDT
                     RQLGAIILQNLGYCLSELRHKDAAAFTGSTLRKSTTPLERVIALIDHLRVVREYSQTA
                     LVPELRQLVLSIAHQSNLSPEDRRDATWVALSWLIPYAGDDAQAIRDQIRHLGIENPL
                     RLSTFSFGSSYGKRTGLVLYSQVTFQAFSTPETWLAP"
     gene            1413..4325
                     /locus_tag="PLD82_02490"
     CDS             1413..4325
                     /locus_tag="PLD82_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64289.1"
                     /translation="MFTTGNLLVLAISFLMILVFRRFDRRRMELSKIKKYSERIIGEY
                     EELAKVKQREFKDATIELDLLTKKARLTLQTADGSLETISKLVSEIDARRDELSAIAA
                     DMKNTEHAAREVRRLQAAITRDESSIRKLLATAEGSRTVLGEIEKRLPAIEKSFHTRI
                     EDKATAWTKEIEARELPRLEKSLETARIRLGEEFEAACADADSRILTRLSAFEARIEA
                     AEEKLSSTLGQHIQTLSSKASAVLDEFRQTRSQHLEEFSSELESRATAAAGSIEETSL
                     AIEVIRADVLSLNDQAASIDGRIKQFVQESKLFDRADRMMERLQNEIQTLTSQMEKCS
                     SEKKAVLELEKRLASVRDERAAVEGLFARLTERKKELQSIENHAAAITGEIDRIEASL
                     QKQQPLFEKTRLMIERMQEELDLAEAGIKKTEALRVDLDEIEAKGGEVRKMLDGISGD
                     KKSLVTLENRIATMGDSVIRLEQSLAASKDEIDNKAATIGRTIDEFTRKSAIFDKAEN
                     LASRLDSEIEALDKKILEVREERDRLGHLKDDLAAVRKDEDAVRTMLATMEKRRDELV
                     AIEKRVEQAGTAVEQFETLLEKKKLDAEKGLEKDSARIREEISRLEKTLSQVREKSLI
                     ETQNSIGSLKSDIKEMDKRIREFAKETALFERADKLSGKLNEDIAELNTRISQLRVDK
                     KDLLELGKQIESIRKEEAEIARMSSSISNDRKQFADLEKRMGELARTSASVETRMQEL
                     SRASSLLQPVETRLTEVADRQSTLQKSLEELAVKDAKLNQSHNRFKKAESFAEKLENQ
                     LELLKANFEQVQKDKGSLETHVEAMQEKSRKLIENEVRINKLLSHFEQMNGMLSDVER
                     RTAQLTIAQDRLSKMENSIRELSGHADVRIEDLSSLSRKIDSFFGIGASTQRAGTDPA
                     KGKASAKSVKEAPTDERKLNEMIFKMHDQGMDPSQIAHIVRRPISDVELRLHVRKE"
     gene            4331..5437
                     /locus_tag="PLD82_02495"
     CDS             4331..5437
                     /locus_tag="PLD82_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HOG64290.1"
                     /translation="MRWLRLRPLLLTALVLDFILLFLLMLSIKYPESLEEIYTRGVFA
                     WYAKCAGWLSDRIPFSIHEAIGIALALALVVAVSRGCKNFLTKKATLSKLILKGAERL
                     FIALSIFAFLFYLLWGLNYFRPRLIDSAQFDAGLVTTDRATYLSEKCLLVLQALENVS
                     PVPDRDLPLFMQSAISHAMPMIGDRPLATSVRVKYFLGGWLSALPSTGVTLPLLPENH
                     ISLDLFDFEKPFIIAHEKAHLQGRALESEANFLALLACLSAEQPWIRRSGVFFLFRML
                     LRSLPPKQRLLYLSRIPHAAWHDFKAPALRMANKSPFIVALFQMFYGNFLKAQRIQDG
                     LANYSAATQLALGYRFRNGKPLDIDTIGRIIKED"
     assembly_gap    5765..5864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5907..6006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6190..6921
                     /locus_tag="PLD82_02500"
     CDS             6190..6921
                     /locus_tag="PLD82_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64291.1"
                     /translation="MTKHIFIIIALLSILTVSSGIALPAIRVNIIAIDTVPETTASIR
                     PENLRWMLSVLNRDFHSFDNRRFANFILGKIITYTESCQLDPTIAATAHGRARWTALR
                     ATGRNGIPRSPLMDPAALNIFLYHDPDSNARSTGAFYNRWISKNGNRQTLWYPTALLH
                     WRVLKNKYEKLVLHEVGHAFGLSHVRPEEPGSRYPSNIMSGDEHDPTLSDPTRGYFFT
                     QSQSAILLRNISFFEKTASHHETDL"
     gene            complement(7043..7391)
                     /gene="ssrA"
                     /locus_tag="PLD82_02505"
     tmRNA           complement(7043..7391)
                     /gene="ssrA"
                     /locus_tag="PLD82_02505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1822 a         2137 c         1754 g         1557 t
ORIGIN      
        1 ccacgaaccc gccgcctgcg aaccgcgcga gcgccgctgc gtcgccgacg tccgcaagcg
       61 acgtgccctc gcgcgtgcgc aggcccagca tcagccgttc caggcgtgcg tccgcgtccg
      121 acagcgtctc ggcgccctcc gagccatgac cggtcttgtg aagcacctcg cggacgacat
      181 tgacggcctc gcacttcctg cccgataccc ccgaagcggc gccgcacacc tggtcggcag
      241 actggtttcg atcataaaaa cggctctctc ccacccggtt ccggaagcag tgatcctcgg
      301 tgtccttgac gggaccggac atttctctcc gtttggctcc ggcttaccgg agagcttgaa
      361 cagattgaaa accacactcg ccaacctgtt cgctccaaca ccttctgcca cggcaaactt
      421 ggaactcggg aaggtccgac aaggcatgga gtcgatcatc ggtttgatcg agggcttctc
      481 cacccttcct gcatcggata taaaggatat tttttccgcc catctcgcac cggccctgga
      541 gggacacatt cgcatcctgc tcagccagaa tctccatgcc cctgcagccc tgccctggct
      601 caaagcatcc gttctgattt cgcgagcccg gaagaattca gacaaaaccg gttttacgaa
      661 ggccacgctc cttctgcttg atgcactgga caagctggac caaccgccgc ttctcgacaa
      721 atcgacgaca ctccatctct acaacgacac acgtcaattg ggagccatca ttctccagaa
      781 tctcggatat tgtctttcag aattgcggca caaggacgct gcggccttta ccggctcgac
      841 cctgcgaaag agcaccacgc ctcttgagcg agtcatcgcc ctgattgacc atctgcgggt
      901 tgtccgggaa tatagccaga cggccctggt tcccgaactt cgtcaacttg ttttgtccat
      961 cgcacaccag tcaaatctct ctccagagga caggcgtgat gcaacctggg tggcgctctc
     1021 ctggctcatc ccgtacgccg gtgacgacgc gcaagcgatt cgggatcaga ttcgccatct
     1081 cggaatagag aatcccctcc gtttgagcac tttctccttt ggcagcagct atggcaagcg
     1141 gactggactt gttctctact cgcaggtcac gtttcaagcc ttctccactc ccgaaacatg
     1201 gctcgcccct tgacgcgcat tccttccacc tgtctcgctt gatccgcatc ctctgtccac
     1261 gttgccgtgt gcggaggatg ttcttcatca cgcttcagtc tatactttag actaagtctt
     1321 tctgtatctt tactctcggc ccgccttttt gtttgacagc ggaatttgca gacccgtaca
     1381 atggttgtgc gtctgatacc gggaggggta acgtgttcac aactggcaat ctgctcgttc
     1441 ttgccatttc ttttctcatg attctcgtct tcaggcgttt tgaccggaga agaatggaac
     1501 tctcgaagat caagaaatac tctgaacgga ttatcgggga atatgaggaa ttggccaagg
     1561 tcaaacaacg cgaattcaag gatgcgacta tcgaactgga tctcctcacc aaaaaggctc
     1621 gcttgaccct gcaaaccgcg gacggttcgc ttgagacaat cagcaaactc gtcagcgaga
     1681 tcgacgcaag acgggacgag ctttccgcta tcgccgcaga catgaaaaac accgaacatg
     1741 ccgcgagaga agtccgccgc ctgcaagccg ccattacccg ggatgagtct tcaatacgca
     1801 aactcctggc tacggccgaa gggagccgga ccgttcttgg tgaaatcgag aagcgtctcc
     1861 cggccattga aaagagtttc cacacccgga tcgaggacaa ggccacggcc tggacaaaag
     1921 agatcgaagc gagggagttg ccacggcttg aaaaatccct ggagacagcc agaatccgcc
     1981 tcggagagga attcgaggca gcctgtgctg atgccgactc ccggatcctc accagattgt
     2041 cggcttttga agcaagaatc gaagcggccg aagagaagct gtccagtaca ctcggccagc
     2101 acatccagac tctttcctcg aaggcctccg cagtcctcga cgaattccgc cagacccggt
     2161 cacagcatct tgaggaattt tccagcgaac tggaatccag ggcaacggct gctgccggct
     2221 ccattgaaga gacaagcctt gcgatcgaag tgatccgtgc cgatgtcctg tcgctgaacg
     2281 atcaggccgc aagcattgac ggccggatca agcagttcgt ccaggagagc aaacttttcg
     2341 accgtgcaga ccggatgatg gagcgcctcc agaacgagat ccagacactg acatcccaaa
     2401 tggagaaatg ctcctccgaa aaaaaagctg tccttgaact tgaaaaacgt ctcgcctcgg
     2461 tccgggatga gcgtgcggcg gtcgagggcc tctttgcacg tctcaccgaa cggaaaaagg
     2521 aactccagtc gatcgaaaac catgcggccg cgatcactgg cgagatcgac agaatcgaag
     2581 ccagcctgca aaagcagcag cctctgtttg aaaaaaccag actcatgatc gagcggatgc
     2641 aggaagaact tgacctcgcc gaagccggaa tcaagaaaac cgaagccttg cgggtcgatc
     2701 tggacgaaat cgaagcaaag ggcggagaag tccgaaaaat gctcgacggc atcagcggag
     2761 acaagaagag cctggtgacc ctcgaaaaca gaattgcaac catgggtgac tccgtcatcc
     2821 gccttgaaca gtcgctggcc gccagcaaag acgaaattga caacaaagcg gccactattg
     2881 gtcggaccat cgatgaattc acacgaaaat ccgccatctt cgataaggcc gagaatctcg
     2941 cttcacgact cgactccgag atcgaggcgc tcgacaaaaa gatcctcgaa gtccgcgagg
     3001 aacgcgacag actcgggcat ctcaaagatg acctcgccgc tgtccgcaag gatgaggacg
     3061 cggtccggac aatgctcgcc acgatggaga agcgacggga tgaacttgtc gctattgaaa
     3121 agcgtgttga acaggccggc accgctgtcg aacaattcga gaccttgctc gaaaaaaaga
     3181 aacttgacgc tgaaaaagga ctggaaaagg attccgcaag aatccgggag gagatatccc
     3241 ggctggaaaa aacactttca caggtccgtg aaaagtccct gatcgaaaca cagaacagca
     3301 tcggatccct caagtctgac atcaaagaaa tggacaaacg catcagggag tttgccaaag
     3361 agacagctct ttttgaacgt gcggacaagc tctccggcaa gctcaacgaa gatatcgccg
     3421 aactgaacac gcgcatcagc cagctcaggg ttgacaaaaa agaccttctc gaactcggga
     3481 aacaaatcga gagcatacgc aaggaagaag cggaaatcgc acggatgtcg tcaagtatat
     3541 ccaacgaccg gaaacaattt gccgatctcg agaagaggat gggcgaattg gcccgcactt
     3601 cggccagtgt tgaaacccgc atgcaggaac tcagccgcgc atcaagcctc ctccagccgg
     3661 tcgaaacccg tctcaccgag gttgcagatc gccagtccac actccaaaaa tccctcgagg
     3721 aactggccgt caaggatgca aaactcaacc agtcacacaa ccgcttcaag aaggccgaga
     3781 gtttcgccga aaagcttgaa aatcagcttg aactcctcaa ggcaaatttt gaacaagttc
     3841 aaaaagacaa aggttccctg gaaacccatg tcgaagcgat gcaggaaaaa tcccgaaaac
     3901 tcatcgaaaa tgaagtccgc atcaacaagc tcctttccca ctttgagcag atgaacggga
     3961 tgctctccga cgttgaacga agaacggcac aactgacaat tgcacaggac cgcctctcga
     4021 agatggaaaa ctccatccgc gaactctccg ggcatgccga tgtccggatt gaagatcttt
     4081 catccctctc ccgaaaaata gattccttct ttggaatcgg cgcctcgaca cagcgtgccg
     4141 ggaccgaccc ggccaaagga aaggcatcag ccaaatcggt caaggaagcc cccaccgacg
     4201 agcgcaaact caatgaaatg atctttaaaa tgcatgatca gggaatggat ccgtcgcaga
     4261 ttgcccacat tgtgcgtcgt cccatctcgg atgtcgaact gcggctgcac gtccgtaaag
     4321 agtagcctga atgcgctggc tccggcttcg ccccctgctg ttgacggctc tcgtgctcga
     4381 tttcatcctt ctgttcttgc tcatgctttc aatcaagtat ccggagagtc tggaggagat
     4441 ctacacccgt ggcgtgttcg cttggtacgc caaatgcgca gggtggctct ccgacaggat
     4501 ccctttttcg atccacgaag ccatcgggat tgccctggct ctggcgcttg ttgtcgccgt
     4561 ctcccgtgga tgcaagaatt tcctcacaaa aaaagccacc ctgtcaaaac tgatcctcaa
     4621 gggcgcagaa cgtctgttta tcgctctttc gatttttgcg tttttattct atcttctttg
     4681 gggattgaat tatttcagac caagactgat cgattccgct cagttcgatg caggtcttgt
     4741 aacgacagac cgcgcgacgt atctctcgga gaaatgcctt ctggtcctcc aagccctcga
     4801 gaacgtttca ccagtccctg atcgggatct accgctgttc atgcagtctg ccatcagtca
     4861 tgccatgccc atgatcggcg accggccctt ggccacatcc gtccgtgtca aatatttcct
     4921 cggtggctgg ctgtcggctc tcccgtcaac tggcgtcaca ctccccctcc ttccggaaaa
     4981 ccatatctcc ctggatcttt ttgatttcga aaagcccttc atcatcgcac atgagaaggc
     5041 ccatctccag ggacgtgcac tcgagtccga agcaaacttt ctggcgcttc tggcctgtct
     5101 ttctgctgaa caaccctgga tccggcggag tggagttttt ttcctctttc gaatgctcct
     5161 gcgctctctc cctcctaaac agagactcct gtatctttcg agaattcccc atgcagcctg
     5221 gcatgatttc aaggcacctg ccctgagaat ggccaacaag agccctttca tcgtggcact
     5281 cttccaaatg ttctacggga atttcctcaa ggcccaacgc atccaggatg gccttgccaa
     5341 ctactcggca gcaacgcagc ttgccctggg ataccgcttc cggaatggta aaccacttga
     5401 cattgatacg atagggagga taatcaagga ggactgattt cgtctcgtga gcaaccacgg
     5461 gcggagtctg tccccactgg agaatttttt gcacaattcg atgattttgt tgtatagttt
     5521 cgatgttatt ccaacttact actctgaggt ttatctgtca tgtctgattc gaccacaaac
     5581 aagaaggaga ctggatttat gggaatcttt gcaaatctca agaagaagcc ggccaaacct
     5641 gccgccaatg aagctgttgc acctgccagc aaaccagcga ctgcagcacc aaaggccgct
     5701 gctcccaaga aggccgctgc tcccaagaag gccgctgctc ccaagaaggc cgctgctccc
     5761 aagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggctg ctgctcccaa
     5881 gaaaaaataa gcttgatctt tgtctannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnngctg ctcccaagaa ggctgctgct cccaagaaaa aataagcttg atctttgtct
     6061 acttggctga ccgaacgcac ctcgatatgg ggtgcgtttt tttcccccat gaaaaaagta
     6121 tcccgggatc tatactacag agatgtgcgt agcgctgttg caccctgact tcaaatacag
     6181 agcatcccta tgacaaagca catcttcatc atcattgctc ttttaagcat ccttactgtc
     6241 tcgtccggca tcgcactccc tgccatccgt gtcaacatca ttgccattga taccgttccc
     6301 gaaacaactg catccatccg acctgaaaat ctgcgctgga tgctatccgt gctcaaccgg
     6361 gattttcact cgtttgacaa ccgccgcttt gccaatttta tccttgggaa aatcatcacg
     6421 tacacagaat cctgccaact tgatcccacg atagctgcaa ccgcacatgg cagggcacgc
     6481 tggaccgccc tgcgagccac tggtcgaaac ggaatacctc gctcaccact catggatcct
     6541 gcagccttga acatctttct gtaccacgat cctgactcaa atgcacgctc gacgggtgcc
     6601 ttctacaaca gatggatttc aaaaaatggt aacagacaaa cgctctggta tccaaccgct
     6661 ttactgcact ggcgcgtttt gaaaaacaaa tatgaaaaac tggttctcca tgaagtcggg
     6721 catgcctttg ggctctctca tgtccgtccc gaagagcccg gctcgagata tccaagcaat
     6781 atcatgtccg gcgatgaaca tgacccgacc ctgtctgatc caacaagagg gtattttttt
     6841 actcaatccc agtcggcaat tcttttgcgg aatatttctt tttttgaaaa aacagcttcc
     6901 catcatgaaa ccgacttata gaatcatcga atactttgga tgtagtggga ggcagactgg
     6961 agccccggag gggcgcaagc gcccccccgc aacaggatgt cgctgtacag caacaataca
     7021 cattaataaa tcatcgaata ctttggaggt ggcgggagtc gaacccgcgt cctgaaacaa
     7081 gaaataagcc cacgtctacg tgtgtagtct gtggatcagg cagtcggctt tccgcggaag
     7141 ccacagacaa caccgcgaat cgcccaaccc gtgcatctcg tcagacaggc ccggatataa
     7201 cccgcatgac cagcctccgt agcggcgttc ttatccggcc cggaggccta ccgaacagaa
     7261 cggcggccat caggccgcga gagagtaaga attgtcagct aattatttag cttcggcctt
     7321 ttttacgggg acaccgacac cccgacacgc tgcgaactct cctacgatcc agtcgaaacc
     7381 ggtacacccc ctcacaattc ttgaagccag actgatgcaa gcttccttct gtatcccaag
     7441 tatacgacaa aagaggcccc ggttttgctc
//
LOCUS       DAOSHE010000052         7268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_8909_length_7268_cov_3.683350, whole genome shotgun sequence.
ACCESSION   DAOSHE010000052 DAOSHE010000000
VERSION     DAOSHE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8909_length_7268_cov_3.683350"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..2369)
                     /locus_tag="PLD82_02510"
     CDS             complement(315..2369)
                     /locus_tag="PLD82_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HOG64292.1"
                     /translation="MATHDGYLADFLDDDGLKTVLVVQDAGQKLQVIEQFGKQRTIKS
                     AQVLCRYPGGAMRSELGTVIPALHAKTEGLAASMDSELLWSQIETHEKDLPVNDIASL
                     WFGQAGSEEISATFRVLADDNLRFKRNGLTFQARTPAQVESLLLTRQREDEKRQREEA
                     IHLWLDELDRGHLPDETEPEGPEDVPQKEAVAEETRCPPEVAAGFSRALLEFLVDRKQ
                     SDVGKIFSDRHPENPYEAAFEVLDRLNLLPPGSNPVSLIAGINPEFSKTTIDEAARLT
                     AQDPSAEGKRLDLTGLAAFSIDDETTREIDDAFTVEYLDGRIRIGVHIADVTALIEQQ
                     TPLDREAQSRTLTIYLPEETYTMFPERIGCDLASLNEGEPRRALSLLADFDPESGALL
                     SHEFALTIIRNTWRMTYDETDRVLAGADHGMDKAIRDAHRIALELRRTRLENKAFQIS
                     RPELRVHVKDDRIILKVNRGSSKAQVLVQELMVLYNSLAGQLAANQSLPVIYRVQSPP
                     EGRDFPTGREIAYEPHTAGALFKRIRPARLSLEPGAHSGLGVPFYIQASSPIRRYADL
                     VMQRQLTAAALGRRPAYEQGELYRILAAAEGTSREISAIERRSIRFWALEFLAREDRA
                     REYTAIAVQPVGGRTLIELDDYPLRALLEAGGTRPKPGECVPVRIATVDAKRDILTFV
                     RA"
     gene            complement(2582..5527)
                     /locus_tag="PLD82_02515"
     CDS             complement(2582..5527)
                     /locus_tag="PLD82_02515"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOG64293.1"
                     /translation="MKLKLFPRGFFAAKPVHVILLFCLGVFILSRDSTIQEHKLFMSW
                     EDVLKRFPGLPSKPDIIPPKFETGTATNLLANPPVPGTDRRALDRFFTKLEAARHGGS
                     VRALHYGDSILWGDNVTQQIRKRLLTHFPNGGRGLVPVTIEDGPHGDYVLGGLTQSGD
                     GWNVDLLITMQYHIMDVGLSLRKYTALRDGAISTLKLWKSEYATGLGILADPSKTGLT
                     VRFILTNNAVVTQKIPKIRTSYTLLTNRIPFKEVQITAARGAVLYGYDLRRGSGVTFS
                     PVIRKGICSHDFPNIGIPAFRRQIADWKPDLFIWHFGKNEAGWDRYTIKGHLSGIDWV
                     ITNVRAAWPQADILLIGPGPRMNKYGGPVRLFPSVPEMCSNQLAMARKHGLGYFDTFE
                     ALGGPKGFLDLVNRGLAMTDYVHLTVNGGNILGDAIADNLLTAWAGWLKTSDDTSLAG
                     LFTKKEIPEHAAAQQQQASSTISFDTVGYGFFLLIVFFAYWLLYYAKLMRVLLLLLAS
                     WFFYMTWNPVFIILIIASSIIDYIAGFLIYAARQRDRAWLARVWLIVSLVSNLGLLFF
                     FKYFNLFSDFLGQLGIVEGNAGMLSLILPAGISFYTFQTMSYSLDIYKGLLKPIRSFL
                     KFNLFVSFFPQLVAGPIVRAREFIPQLVARPRFDPALITNGFFMIMVGLFKKVVISDF
                     LAVNFADRVFVSPGIYSPLEVLLGVYAYGLQIYCDFSGYSDIAIGSAAMLGFRLPLNF
                     NSPYKAHTLQDFWHRWHITLSTWLRDYLYIPLGGSRKGNSRTYINLAITFLLGGLWHG
                     AAYRFVIWGALHGLGLGVVRWFQRWRERRARRRAEITGLAIRRGPTPTGRVFGWLLTI
                     NFVMFTWIFFRAPDMKTVGDILAKLGDLPSMLPDMIAMFNTGLSSAFAELATHFDKVA
                     PNVTTAIGLALFGGYALHLVPNRIFERTRSFWMVLPAPFQAIVLLLFMGLLFKTATAA
                     MVPFIYFQF"
     gene            complement(5726..6859)
                     /locus_tag="PLD82_02520"
     CDS             complement(5726..6859)
                     /locus_tag="PLD82_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64294.1"
                     /translation="MSGILVSTGELSASNAAAALGPELLRLAPEIQLRALGSRSLAAS
                     GYAVLTDPTATASIGHWEALARARNRRRLLGESLGLVLHNPPDLVVCIDSPDFNIPLA
                     RAMHRQKIPVLWYIPPQEYLWGATGLARRIGRLASHVLCVDNAAARLYGGLGIPCTVS
                     GHCLAHPGTRPAPAPDRPTGPLIALFPGSRKQEMQRIAPAVIHAAALIARRSPGVRFA
                     LCLADERYRRTLEDLATTSHIPLEIVAREQAGELLNTADLVITKAGTTTMDATFAGCP
                     MVVVYRIHPLTYWIATRILRIQRHLAGISLPNIALGQKAIPELIQHACTPESIATAAL
                     SVLADPQPIREALRTAANLMARPAAASETARVILEFLGTTGRP"
BASE COUNT         1433 a         1905 c         2491 g         1439 t
ORIGIN      
        1 tggcgacttg ttcccgttcg ctgctcttgt tcatggtttg ctccgaggtg gcatggacta
       61 cgattgaaca tcggcagaat tttcaggctg gttgaggtcc ggttttctca aatttgagaa
      121 aaatgtgtcc cggattaaca ccgggtaatt tttgcacagg ttttggcata gagatgcgat
      181 tgggattgct ccgaaacgta ctgttgtcat gcgttgccgg atacctatgg caaaatcacc
      241 ttaaactcat cccaatcccg gattcgcatg acaattctgc aatgtgcgcg ggatgtgcag
      301 ttctgtctgc gcactcatgc ccggacaaag gtcaggatat cccgcttggc gtcgacggtg
      361 gcgatccgga ccgggacgca ttcgccgggt ttgggccggg ttccgccagc ctcgagcaaa
      421 gcccggagcg ggtagtcgtc cagttcgatc agggtccggc ccccgacagg ttgcacggcg
      481 atggccgtgt actcccgggc cctgtcctcg cgagcgagga attcgagggc ccagaagcgg
      541 atggagcggc gctcgatggc ggagatctcc cggctggtgc cttccgccgc cgccagaatc
      601 cggtagagtt cgccctgttc gtaggccggg cggcggccca gtgcggcggc cgtcaactgg
      661 cgctgcatga caaggtcggc gtagcgtcgg attggggaag aggcttgtat gtagaatgga
      721 acgcccaggc cggaatgcgc gccgggctcc agcgagaggc gcgccggacg gatgcgcttg
      781 aagagggccc cggcggtgtg gggttcgtag gcgatttcgc gcccggtcgg aaaatcgcgg
      841 ccttcgggcg ggctttggac ccggtagatc accgggagag attgattggc tgcgagctga
      901 ccggcgaggc tgttgtagag caccatcagt tcctggacca aaacctgggc cttcgaactc
      961 ccccggttga ccttgaggat gatccgatca tctttgacgt gtaccctgag ttccggtcgt
     1021 gaaatctgga aggccttgtt ttcgaggcgg gtccggcgca attccagagc gatgcgatgg
     1081 gcgtcccgga tggccttgtc cattccgtga tctgcgccag caagtacgcg gtcggtttcg
     1141 tcataggtca tgcgccaggt gttgcggatg atggtcaggg cgaattcgtg cgaaaggagt
     1201 gcaccgctct ccgggtcgaa gtcggccagg aggctcaggg cgcgccgggg ctccccctcg
     1261 ttgagactcg ccagatcgca gccgatccgt tcgggaaaca tggtataggt ttcttcgggg
     1321 agatagatcg tcagggtgcg gctttgggct tcgcggtcca gcggtgtctg ctgctcgatc
     1381 agggcggtca cgtccgcaat gtggacaccg atccggatcc gtccgtcaag gtattcgacc
     1441 gtgaaggcat cgtcgatttc acgcgtcgtc tcgtcgtcga tgctgaaggc ggccagcccc
     1501 gtcaggtcca gccgttttcc ctcggcggat ggatcctggg cggtcagcct ggcggcctcg
     1561 tcgatggtcg ttttggaaaa ctccggattg atgccggcga tcaggctgac cgggttgctt
     1621 cccggtggca gcaggttgag cctgtccaga acctcgaagg cggcttcgta ggggttctcg
     1681 gggtggcgat cggaaaatat ttttccgaca tcggattgtt tgcgatcgac aaggaactcg
     1741 aggagtgccc gggagaatcc ggccgcgacc tcggggggac agcgcgtctc ttcggcaaca
     1801 gcttcttttt ggggaacatc ctccggccct tccggttccg tctcgtccgg gagatggccg
     1861 cgatcgagtt cgtccagcca gagatggatc gcctcttccc gctggcgctt ttcatcttcg
     1921 cgctgtcgtg tcaggaggag agattcaacc tgcgcgggcg tgcgggcctg gaaggtcagg
     1981 ccgttgcgct tgaagcgcag gttgtcgtcc gctagaaccc tgaatgttgc cgagatctcc
     2041 tcggatccgg cctgaccaaa ccagaggctg gcaatgtcgt tgaccggaag atccttttcg
     2101 tgagtctcga tttgggacca gaggagttcg ctgtccatcg atgcggcgag tccctccgtt
     2161 ttggcatgga gggcggggat gactgtcccc agttcgctgc gcatcgcgcc gcccgggtag
     2221 cggcagagga cctgcgcgga cttgatggtg cgctgtttcc cgaattgctc gatgacctgg
     2281 agtttttgac cggcgtcctg taccacgagg acggtcttga gtccgtcgtc atcaagaaaa
     2341 tcagccagat atccgtcgtg tgtcgccaaa acaggctcct ctgtgagtgt cagataaaag
     2401 tacgaaacat gccctgcctg cggaactccg gaagacgagg gatggcaggg catccccggt
     2461 ggaacaatat tccgggacag ggcgagcccg gtgtgactga ggcggttcac caaaaagaac
     2521 ctgactgccc ggcgccggga cccgaaaaac cggcacccct ccccggaggg agggatgcgc
     2581 cttaaaactg gaagtagatg aaaggaacca tggctgcggt tgcggtcttg aagaggagcc
     2641 ccatgaagag caggaggacg attgcctgga agggggccgg gagaaccatc cagaagctgc
     2701 gggtccgctc gaagatccgg ttggggacca agtgcagggc atatccgcca aagagtgcca
     2761 gtccgatggc ggttgtgaca ttgggggcga ctttgtcaaa gtgggtcgcc agttcggcga
     2821 aggccgatga gagaccggta ttgaacatgg cgatcatgtc ggggagcatc gagggcaggt
     2881 cccccagttt ggccaggatg tctccgacgg tcttcatgtc tggggcgcgg aagaagatcc
     2941 aggtaaacat cacgaagttg attgtcaaca gccagccaaa aacccgtccg gtcggtgttg
     3001 gcccgcgacg gatggcgagt cccgtgattt cggcccggcg ccgggcgcgt cgttcacgcc
     3061 agcgctggaa ccagcggaca accccgagcc cgagtccgtg gagggcgccc cagatcacga
     3121 agcggtaggc cgcgccgtgc cagagtccgc cgaggaggaa tgtgatcgcg agattgatat
     3181 aggtccggga atttcccttg cggcttcccc cgagcgggat gtagaggtag tcccgcagcc
     3241 aggtcgagag ggtgatgtgc cagcggtgcc agaaatcctg gagggtgtgc gccttgtagg
     3301 gggagttgaa gttgagcggg agcctgaagc cgagcatggc cgcggagccg atcgcgatat
     3361 cggaatatcc tgaaaaatcg caatagatct gcagaccgta ggcatagacc ccgagcagaa
     3421 cctcgagcgg ggaatagatg ccgggcgaga cgaagacccg gtcggcgaaa ttgacggcca
     3481 gaaaatcgga gatgaccact tttttgaaga ggccgaccat gatcatgaaa aacccgttgg
     3541 tgatcagggc gggatcaaag cggggccggg cgactaattg cgggatgaat tcccgggcgc
     3601 ggacgatcgg tccggcaacg agttggggga agaaactgac aaagaggttg aacttgagga
     3661 aggaccggat cggtttgagg agccctttgt agatgtccag ggaatagctc atcgtctgga
     3721 aggtgtagaa cgagattccg gccgggagga tcagggagag catccccgca tttccttcga
     3781 cgattccgag ctgacccaaa aagtcgctga agaggttgaa gtacttgaag aagaacagga
     3841 gaccgagatt ggatacaagg ctgacgatca gccagacgcg agccagccag gccctgtcgc
     3901 gctgacgtgc cgcgtagatc aggaaaccgg cgatatagtc gatgatgctg gaagcgatga
     3961 tcaggatgat gaagaccgga ttccaggtca tgtagaagaa ccagctcgcg agcaggagca
     4021 ggaggacccg catcagtttg gcgtaataga gaagccagta ggcgaaaaac acaatcagca
     4081 ggaagaagcc gtagccgacc gtgtcaaagg agatcgtcga actggcctgc tgctgttgcg
     4141 ccgcagcgtg ttcaggaatt tctttttttg tgaagagtcc ggcgagggat gtatcgtcgg
     4201 aggttttgag ccaaccggcc caggcggtca ggagattgtc ggcgatggca tcgccgagga
     4261 tgttcccgcc gttgaccgtc aggtggacat agtccgtcat cgccaggccg cggttgacca
     4321 ggtccaaaaa gcccttgggc ccgcccaggg cttcaaaggt gtcgaaatac cccagaccgt
     4381 gcttgcgcgc catggcgagc tggttggagc acatctccgg aaccgaaggg aagagccgca
     4441 cggggccgcc gtacttgttc attctcgggc ccgggccaat caggaggatg tctgcctgtg
     4501 gccaggcggc ccggacattg gtgatgaccc agtcgatgcc cgagagatgg cccttgatgg
     4561 tatagcggtc ccaaccggct tcgtttttcc cgaaatgcca gatgaagagg tcgggcttcc
     4621 agtcagcgat ctgccggcgg aaagcgggga tcccgatgtt cggaaaatca tgggaacaga
     4681 tgcccttgcg gatgaccggg gagaaggtca cgcccgaacc gcgtcggaga tcgtacccgt
     4741 agagcaccgc cccccgtgct gctgtaatct ggacttcctt gaagggtatg cggttggtga
     4801 ggagggtgta gcttgtgcgg attttcggga ttttttgtgt gacgacggcg ttgttggtca
     4861 ggataaagcg caccgtgagt ccggttttgg aaggatcggc caggattccc aggccggtgg
     4921 cgtattcgga tttccagagt ttgagggtgc tgattgcccc gtcccggagg gcggtgtatt
     4981 tgcgcaggct gagacccaca tccatgatgt ggtattgcat ggtgatcagg agatcgacat
     5041 tccagccatc accggactgg gtcagccctc caagaacgta atcgccatgg gggccgtcct
     5101 caatggtcac aggaaccagt ccccgtcctc cgttgggaaa atgggtcaga aggcgtttcc
     5161 ggatctgttg ggtgacattg tccccccaga gaatggaatc accatagtgc agggcccgga
     5221 cgcttccccc gtggcgggcc gcttcgagct tggtgaaaaa ccggtccagc gcccggcggt
     5281 ccgttccggg gacgggcgga ttggcaagga ggttggtggc ggttcctgtc tcgaattttg
     5341 gcgggatgat gtcaggcttg gagggaaggc ccggaaagcg tttgagaaca tcctcccagg
     5401 acatgaagag tttgtgttcc tgaatcgtcg agtcccgtga gaggatgaag actcccagac
     5461 agaagagcag tatgacgtga acgggttttg cggcaaagaa cccgcgggga aagagtttca
     5521 atttcatgga cagtgttccg gtttgaagta tccaaaaaat accacatttc cggccggccg
     5581 ggcaggctgg tccggtcggc caggtcaggg tgcatgcccg acctggtgaa cggaatgtcg
     5641 tgacgggagg ggctgtttca gtccggggca aggagcaaga ggcgttttgt ggggggcctg
     5701 cgacacaagg cagccctgtc gggcgtcatg gccgtccggt tgtgccgaga aattcgagga
     5761 ttacccgggc tgtctcgctg gcggctgcgg gacgggccat cagattggcg gctgtgcgca
     5821 gggcttcgcg gatcggttgt ggatcggcga ggacggacag agcggctgtg gcgatggatt
     5881 ccggcgtgca ggcatgctgg atcaactccg ggattgcttt ctggccaagg gcgatgttcg
     5941 ggagactgat tccggccagg tggcgctgga tccgcaggat gcgtgtggcg atccagtacg
     6001 tcagggggtg aatgcgatag acgacaacca tcgggcagcc ggcaaaggtc gcatccatgg
     6061 ttgtggtccc ggccttggta atcacaagat ccgcagtatt gagaagctcg cccgcctgtt
     6121 cgcgggcgac gatctcgagg gggatatgac tggtggttgc caaatcctca agagtccggc
     6181 gatagcgttc gtcagccaga cagagggcaa accggacgcc gggggagcgc cgggcgatca
     6241 gggccgcagc atggatgacg gccggggcaa tccgctgcat ctcctgtttg cggcttccgg
     6301 ggaagagcgc gatcagcggg cctgtgggcc ggtcaggagc gggggccggg cgggtcccgg
     6361 ggtgtgcgag acaatggccg gagaccgtgc agggtatgcc cagcccgcca tataagcggg
     6421 cggcggcgtt atccacgcaa aggacgtgcg aggccaggcg ccctatgcgg cgggccagtc
     6481 ccgtggcccc ccagagatat tcctgcggag gaatgtacca gaggacgggg attttctggc
     6541 gatgcatggc ccgggccagg ggaatgttga aatccgggct gtcgatgcag acaaccagat
     6601 cgggagggtt gtgcaagacc aggcccagac tctcacccaa gagacgccgg cggtttctgg
     6661 cccgggccag agcttcccag tgcccgatcg acgcggttgc ggtcgggtct gtgagaacgg
     6721 catagccgct tgctgccaga ctccgggagc ccagggcccg gagctggatt tccggagcga
     6781 ggcggaggag ttccgggccg agggcggcgg ccgcgttgga cgccgagagt tcccctgttg
     6841 agacaagaat cccggacatg caaacctcgt ttgaacctgt caactgattc ggggagtggc
     6901 ggtattcagt ccctctgcgt aagggaaaga aagcatacag gctgggctgt tgcccaaaag
     6961 agaggatgac tcccgtttgg ggatatgcct gatggattct gttgctgttc cagaaagatc
     7021 aggcgttgac aacgtaccat gccagtgtgc tttcgagggg atacgtgcac tgcccggaca
     7081 gggaagatgt aagccgttga cggcatgtat attatacagg aagaaattgc gggagggcgc
     7141 atgtttcgtg actggttggc atggctgtat gttccgtatc gtgtctgggc gaggatcgcc
     7201 acgtcccctc tcgggctccg ccgcgttttc gtccgtgctc tccttccttg cgcgatcctt
     7261 cccttggt
//
LOCUS       DAOSHE010000053         7200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9030_length_7200_cov_4.362071, whole genome shotgun sequence.
ACCESSION   DAOSHE010000053 DAOSHE010000000
VERSION     DAOSHE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_7200_cov_4.362071"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLD82_02525"
     CDS             <1..875
                     /locus_tag="PLD82_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG64295.1"
                     /translation="GSLKTIRNIEADIAIDIGQWSRTSAILARCSGAVERIGFTRKWQ
                     GRRAGFTTTVCHQNTCHEVVNFFNLFSALHPSAGFSVSDCTPSISPPADFTSSFPETN
                     ADHVPDQLLVLHLFPGGTASSMKELNGEQSSTLIKALISAGYRIALTGGPENTLRAAE
                     IIRAASFPEKKLTSACGWRVRETANLIRRARAVISVNTGIMHLAAAIGTPLVAIHGPT
                     NPVRWGPLGDKKTIRVVSSQAGCLACLDLGGEYACHPGREQPACMRAVVTSDILSALE
                     SVISGHTRKGDRPF"
     gene            1006..2592
                     /locus_tag="PLD82_02530"
     CDS             1006..2592
                     /locus_tag="PLD82_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG64296.1"
                     /translation="MLGNLKIGLRLTLSFAIILVMIGVMVFVSILSMNDVQNKLVRIV
                     TVNSAKMALANDLLDLHGIRINAIKNFVIFSDDKAMADEMKTLEHARAESDKCEQKLL
                     AMEMTTNERAILTSYQDQMKTWLGINTRLLEAAKKNDIKACEAIIHNESEPAGGRALK
                     LLHDLINYQTQRTAMRYEESKDAYQSAFTLLLVLSAVVLFLTIVLAFFLTRSITKPLA
                     AMVLRVQDIAEGDGDLTQRVDLSSKDELGVLAGWINKFIENLAKDVRGIAEASGEVAT
                     AAGETNQVSESLAAATEQISQQAQTISAGAAEMNQALQTIASSIEEMSISVGEVARKA
                     AEAAQATGEANTAAAQMDVVVNELGSHAQAIGRVVELIGTIASQVNLLALNAAIEAAS
                     AGDAGRGFAVVAAEVKELAEQASTSTDEIKQTVALIQKSTGGTMENMKVIRSVIDRVD
                     QISGAIASSVEEQAITAKEIAGNVNQTSVASNEVSQNISGIAEASRSGAEGAIKVNGI
                     ATTVVDRANRVASIVSKFKT"
     gene            complement(2753..3616)
                     /locus_tag="PLD82_02535"
     CDS             complement(2753..3616)
                     /locus_tag="PLD82_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64297.1"
                     /translation="MKIFFHLDPDGCCAAAIAARYYNGEGKYIAWRHEMSFPYDEIEK
                     DERVVVLDINLPPERDRLWERTHDIVWVDHHISAIEKNGPWATELGMPGIREVGEAAC
                     VLAWRHFFPEQAMPSIVALIGDQDIWRFEFGDDTRALHYGLDLVDISPKSVFWEGYLV
                     PGSGIDTLEPLLVPGRAIHAYESKRRARMVREYAYETEIDGYKAVACNLRGNSAMFEE
                     LEKTYDVCIAYMYNGERYQISLYTLRDDIDVSKTCEKFGGGGHAKAAGFFSDEIPWPL
                     AGKRLIRKSKP"
     gene            4011..4343
                     /locus_tag="PLD82_02540"
     CDS             4011..4343
                     /locus_tag="PLD82_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64298.1"
                     /translation="MILTQDIKDWMEKISYSERISQNEVLRMALEWWMEAANPLASGR
                     SFRAACRKWYHDRIISRPEEFFFHFWQYARIKYDQYPLEEETLSAIRANFPSRDSPHT
                     IERGKLLY"
     gene            complement(4432..5202)
                     /gene="trpA"
                     /locus_tag="PLD82_02545"
     CDS             complement(4432..5202)
                     /gene="trpA"
                     /locus_tag="PLD82_02545"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG64299.1"
                     /translation="MTHAVGGWPDMETSEAILCAMIDAGADILEIQLPFSDPAADGSM
                     IVAANHQALANGATTKKILAMIGRVRRHADTRAGGGVTSILVMSYLNPVLAYGEHAFV
                     QDASEVGVDGFIIPDCPPDEKDMLPEISSAAGLAFVPLIAPDTDDARMEMICRRFISP
                     LVYAVLRYGVTGRKSVIDAGTKKYLERIQRVTGRALAAGFGIRERDQVEALQGVVDCF
                     VAGSVFISTVTDAIASGKSPAEAVQKHIVALQGQDQNG"
     gene            complement(5412..6758)
                     /locus_tag="PLD82_02550"
     CDS             complement(5412..6758)
                     /locus_tag="PLD82_02550"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012670314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG64300.1"
                     /translation="MESMDKRYYGEYGGRYIPEVLVPAFTQLEEAWSRLGQDKGFLDD
                     LARLQRDFIGRPTPLLFAENLTRVCGGGRIWLKMEALAHTGAHKINNALGQGLLAKKM
                     GKKRIIAETGAGQHGLATAAVAARLGLECEVFMGEVDMARQHPNVYGMRLFGATVTPV
                     TEGSKTLTDAVNAAMKNWTERVSDTHYLLGSALGPYPFPEIVRTFQSVIGREVRLQFP
                     ERYEELVVRQGQSGSGMREGNSMSSESAKPVSVKTLPDVLVACVGGGSNSIGLFHEFI
                     EDGQIRMVGVEAGGQGDGPGEHAVRMSSGQGRPGVVQAYRSIFLQDENGSLLPTSSIS
                     AGLDYAGIGPELAYYGKSGRIEFRSARDEAVLEAVLLLSRTEGILPALESAHGLAAAL
                     DIAKESSPSTDIVVNISGRGDKDLFITAPRLDGEDWLQFLKSEVARIEGGPGKMVP"
     gene            complement(6758..>7200)
                     /locus_tag="PLD82_02555"
     CDS             complement(6758..>7200)
                     /locus_tag="PLD82_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64301.1"
                     /translation="LAAYRSVPWYPVLPLAVGGAKGLERAAEQEYRFAFWGTTDIVLG
                     DAAVAGMAGGSGVRVATDAACAARDFVHRAGKLFFLAGGLRPETVLDVLGHISPDGID
                     VSSGLEESPGRKSKEKIQVFLRHVREWEHQQDQASKPLTGRRER"
BASE COUNT         1760 a         2148 c         1731 g         1561 t
ORIGIN      
        1 tggggagttt aaagaccatc aggaacatcg aagcggatat cgccatcgat atcggacaat
       61 ggtcccggac cagcgccatc ttggccagat gttccggcgc ggtcgaacgg atcggattca
      121 cccgaaaatg gcagggacgc cgtgccggtt tcacaaccac ggtctgccac caaaacacct
      181 gccacgaggt cgtcaatttt ttcaatttat tttccgcact tcacccgtct gccggttttt
      241 cagtcagcga ttgcaccccc agcatcagcc cacctgccga tttcacatcc tccttccctg
      301 aaacgaacgc ggaccatgtc ccggatcagc tacttgtgct gcatcttttt cccggcggca
      361 cggcttcatc gatgaaagaa ttgaatgggg aacagtcctc aaccctgatc aaagccttga
      421 tcagcgcggg gtacaggata gccttgacgg gtggcccgga aaacacgctt cgagccgctg
      481 aaatcatccg ggctgcatcg ttcccagaaa aaaaactgac cagtgcctgc ggatggcgtg
      541 tgcgggaaac ggccaatctg atccggagag cgcgggctgt gatctccgtc aataccggca
      601 tcatgcatct ggctgccgcc atcggcacac cacttgtcgc catccatggc ccgacaaacc
      661 ccgtgcgctg gggtccgctt ggcgataaga aaacgatacg cgttgtttct tcacaggcgg
      721 gctgtcttgc atgtctggat ctcggagggg aatatgcctg ccaccccgga agagaacaac
      781 ccgcctgcat gagagctgtt gtgacaagtg acattctttc cgctctcgag agtgtaattt
      841 ccggtcacac caggaaagga gacaggccgt tttgaccacg ctcatcccgg aaatcaggcc
      901 gaaaaattca gtagaaatac ggattgaaga tccagaaata atacggcaga ataggacgtg
      961 tcaaaattgc ccggaattgg atgggcccac tcaagaggag tgaatatgct cggaaatttg
     1021 aaaatcggtc tacggctcac gctgtccttt gccatcattc ttgttatgat tggagtaatg
     1081 gtttttgtca gcatcctgtc aatgaacgat gtgcagaaca agctggttcg catcgtgact
     1141 gtcaacagtg cgaagatggc actggccaac gatttgttgg atcttcatgg catccgcatc
     1201 aatgccatca aaaatttcgt catcttctcg gatgacaaag ccatggcgga cgagatgaaa
     1261 acgctggaac atgcacgtgc cgagagcgat aaatgcgagc aaaagctctt ggcgatggaa
     1321 atgacgacca acgaacgggc catattgact tcgtaccagg atcagatgaa aacctggctc
     1381 ggtatcaata cccgtctcct cgaggctgcc aaaaaaaatg acatcaaggc ttgtgaagcc
     1441 attatccaca atgagtccga accagccggg ggcagggcac tcaaactcct gcatgacctc
     1501 attaactatc agacgcaacg aacagccatg cggtacgaag agtccaagga cgcataccaa
     1561 tcggctttca ccctgcttct tgttctctcc gctgttgtcc ttttcctgac catcgtcctg
     1621 gcctttttcc tcacccgcag catcacaaaa cccctggccg ccatggtact tcgtgtccag
     1681 gatatcgccg aaggggatgg cgacctgacc cagagagtcg atctctcctc aaaagacgaa
     1741 ctgggtgtct tggccggctg gatcaacaaa ttcatcgaga acctggccaa agatgtccgc
     1801 ggtattgctg aagcatccgg cgaggtcgcc accgcagcag gcgagacaaa ccaggtcagc
     1861 gaaagccttg ctgcagcaac cgagcagatt tcgcagcagg ctcagaccat atcggccggg
     1921 gccgccgaga tgaatcaggc actccagaca attgccagct cgatcgaaga aatgtccatc
     1981 tccgtaggcg aagtcgcccg aaaagcagcc gaagcggcac aggccaccgg ggaagccaac
     2041 acagccgccg cacagatgga tgtcgttgtc aacgagctcg gttcccatgc ccaggccatc
     2101 ggcagggttg tagaactgat cggtaccata gccagccagg tcaatctgct cgccctgaat
     2161 gccgccatcg aagccgcgtc tgcgggagat gccggtcgcg gttttgccgt tgtcgccgct
     2221 gaggtcaaag agctcgccga acaggcctca acctcgaccg atgaaatcaa gcagaccgtc
     2281 gccctgatac agaagagcac cgggggcacc atggaaaaca tgaaagtcat ccggagtgtc
     2341 atcgaccggg ttgatcagat cagcggagcg attgccagct cggtcgagga acaggctatc
     2401 accgccaaag aaatcgccgg aaatgtcaac cagacttctg ttgcatccaa tgaagtctcg
     2461 cagaacatca gcggaatcgc ggaggcctcg cgaagcggcg ccgagggggc gatcaaggtc
     2521 aacggaatcg ccacaacagt cgtcgaccgt gccaatcgtg tggcaagtat tgtctcgaag
     2581 ttcaaaacct gaaattcgaa acatctttct gtcagattcc acccggccac aaactggccg
     2641 ggttttttca agtttcatca tgctgacggg gtatgcgaac ggaacttgca gcgcagactg
     2701 tcgcatccct gtccacgctg tcctcaagca ccgctcagtc aaaaccaaaa acttagggct
     2761 tgctcttcct gatcaaacgc ttgccggcaa ggggccatgg aatttcatcc gaaaaaaatc
     2821 cggcggcttt ggcatgtcca ccgcctccga atttttcgca tgtcttcgag acatcaatat
     2881 catccctcaa cgtatagagc gaaatctgat accgctcgcc gttgtacata taagcgatgc
     2941 agacatcata ggtcttttca agttcctcaa acatggccga gtttccgcga agattgcagg
     3001 ccacggcctt atagccgtca atctcggttt cataagcata ttcacggacc attcgtgctc
     3061 ttcgttttga ttcataggca tggatggcac ggcccgggac aaggagtggt tcaagcgtgt
     3121 cgataccgga tcctggaaca agatatcctt cccaaaacac acttttcggg gagatatcga
     3181 caagatccag tccataatgc agtgcccgtg tgtcatcacc aaattcgaat cgccatatat
     3241 cctgatctcc gatcagggcg acaattgaag gcatcgcctg ttccggaaaa aaatgtcgcc
     3301 aggccaaaac gcaggccgct tcaccgactt cacgaattcc gggcatgccc agttccgtag
     3361 cccatggacc gtttttttca atggccgaaa tatggtgatc gacccagacg atatcatgcg
     3421 tcctctccca caaacgatct ctttcgggcg gaagattgat atccaacacg acaacccgtt
     3481 catccttttc tatctcatcg tacgggaaac tcatctcgtg ccgccatgcg atatacttgc
     3541 cctccccatt gtaataccgt gcagcaatcg ctgccgcaca acatccatcg ggatcaagat
     3601 ggaaaaaaat cttcatgtca gcgtccttgt ttcaagtatg gtgcagatca gcacacatgt
     3661 acccgggaaa gatagtcaaa tcccgcaact gtgcatttca gcaatattca tgagaaacat
     3721 ggcaccatat gcgcagcccg gtcctctcct gtatgaaaac ggaggacaca tacatggatg
     3781 tcaatctgtc ggatgtggca agagtacgca catacggaaa aattgttttc cgctgccgtc
     3841 tgcaaaaatc ggcaaacacg ttctacagcc agcactggat gcacttctca aaagcatgcc
     3901 gtcgggctct cacgcgtttt gcacaggctc tcaaaggagg cacactccgt atcgaagagc
     3961 gaagccggtt tctgacacga cgggatcttt cggacaaggt cacacgctct gtgatcctca
     4021 cacaggacat caaagactgg atggagaaaa tctcgtattc ggagcgaatc agccaaaacg
     4081 aggttcttcg tatggcgctg gagtggtgga tggaggcggc gaatccgctt gcctcgggcc
     4141 ggtccttccg ggctgcttgc agaaaatggt accatgatcg cattatctcc aggcccgaag
     4201 aattcttttt ccacttctgg caatacgcac gtatcaaata cgaccaatat ccattggagg
     4261 aagaaacgct atccgcaata cgagccaact tcccgtctcg tgactcgcca cacaccatcg
     4321 aacgcggaaa attattatac tgacaggcag gccgtctcca tccgaaaatc gctttcagaa
     4381 aaaagctgaa gacatacagc gcttggcata ctctcctgtg cattctgtca ttcagccatt
     4441 ttgatcctgc ccttgcagcg caacaatgtg tttctggact gcctcagctg gcgactttcc
     4501 ggaggcgatg gcatcggtga ctgtcgagat aaaaacactt cctgcgacaa aacaatcgac
     4561 aacaccctgg agcgcctcga cctgatcacg ctcccgaatc ccgaaccccg cggccagcgc
     4621 acgacctgtc accctttgta tccgttcaag gtacttcttc gtcccggcgt caatgaccga
     4681 ttttcttcca gttacaccat accgcaggac agcatataca agcggggaga tgaaccgccg
     4741 acatatcatt tccattcttg cgtcatcggt atctggagca atcagtggta caaatgccaa
     4801 ccctgccgca ctcgatatct ccggcagcat gtctttctca tcagggggac aatcagggat
     4861 aatgaaaccg tcaactccca cttctgatgc atcctggaca aacgcatgct caccataggc
     4921 gagaacagga ttcaggtaac tcattaccaa tatggatgtt acaccgccac cagcgcgtgt
     4981 atcggcatgc cttcgcactc tcccgatcat cgcaaggatt ttttttgtcg tcgcaccgtt
     5041 cgccaaggct tgatgattgg ccgcaacgat catcgaccca tctgcagcag gatcagaaaa
     5101 cggaagctgg atctcaagaa tatccgcacc tgcgtcaatc atcgcgcaca ggatggcctc
     5161 cgatgtttcc atatcaggcc agccaccgac agcatgtgtc atgcaccgca atcctgttgt
     5221 attttcctga aaaatctgtc gtattctatc gattcctgtc acagaatccc ccttgccata
     5281 cttgttcctc aaaaaacggt gccatgacat cggtcacccc gatctgcact accccatcac
     5341 ctcatggcat cagtatctga tgccgtcaca cagaagcaac gatacccggg tgcagtgcca
     5401 aaaatatgcg cttatggcac catttttccc ggaccaccct caatcctggc gacctcgctt
     5461 ttcagaaact gtaaccagtc ctcgccatcg agcctcggag cggtaatgaa cagatctttg
     5521 tcaccccgac ctgagatatt cacgacaatg tccgttgatg gtgacgattc cttcgctatg
     5581 tccagtgccg cagccaggcc atgggcagac tccaacgccg gtaaaatccc ttccgtcctt
     5641 gaaagaagaa ggaccgcctc caacactgct tcatcccggg cggacctgaa ttctatcctc
     5701 ccggattttc cgtaataggc cagttccgga cctatcccgg catagtccag cccggcagag
     5761 atggatgagg tcggaaggag tgacccgttc tcatcctgga gaaaaatgga tcggtatgcc
     5821 tgaacaaccc cgggacggcc ctgcccgctc gacattcgca ccgcatgttc cccgggcccg
     5881 tctccttgcc caccggcctc aacgccgacc atcctgatct gtccgtcctc gatgaattca
     5941 tggaagagcc cgatggaatt ggagccaccg ccgacacagg cgacaaggac atccggaagc
     6001 gtcttcaccg ataccggctt ggcgctctcg gacgacatgc tgtttccctc acgcatgcca
     6061 ctaccacttt gcccctgccg cacgaccaat tcctcatatc gttccggaaa ctgcaaacgg
     6121 acctcccgtc cgatcacaga ctgaaaagtc cggacaatct ccggaaacgg atatggaccc
     6181 agcgctgaac caagaaggta gtgggtatcc gagaccctct ctgtccagtt tttcatcgca
     6241 gcattcaccg catcggtcag tgttttcgaa ccttccgtca caggagtgac cgtcgcacca
     6301 aagagtcgca taccatagac attcggatgc tggcgggcca tatcgacctc gcccatgaat
     6361 acctcgcact ccaacccaag ccgtgccgcg acagcggctg ttgccaatcc gtgttggccg
     6421 gcaccggtct ccgcgataat tcttttcttg cccatttttt tggccagcaa accctggccg
     6481 agcgcattgt tgatcttgtg cgctccggta tgcgccaggg cttccatctt caaccagatc
     6541 cgcccgcctc cgcacacccg tgtcagattc tccgcgaaca ggaggggcgt gggccggccg
     6601 ataaaatccc tttgcagacg tgccaagtca tccagaaagc ctttgtcctg tccaagacgg
     6661 ctccaggctt cttccaattg tgtaaacgct ggaaccagaa cctccggaat atatctgccg
     6721 ccatactctc cgtaatatct cttgtccatg ctttccatca tcgctcccgc cttcccgtca
     6781 atggtttcga tgcctgatcc tgttgatgtt cccattcgcg aacgtgacga agaaacacct
     6841 gtattttttc tttcgacttt cttccgggag attcctcgag cccgctcgac acatcgattc
     6901 cgtcagggct gatgtgtccc agaacatcca gaaccgtctc cggacgaaga ccgcccgcaa
     6961 gaaaaaacag ctttccggcc cggtggacga aatccctggc ggcacaggct gcatccgttg
     7021 cgacacgaac gccgctcccg cctgccatac ccgcgacagc cgcatccccg agcacgatgt
     7081 ccgtggttcc ccaaaatgcg aacctgtatt cttgttccgc agcacgctcc aggccctttg
     7141 ccccaccaac tgccagcggg agaaccggat accagggtac ggatcggtat gccgccaatt
//
LOCUS       DAOSHE010000054         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9037_length_7198_cov_4.443371, whole genome shotgun sequence.
ACCESSION   DAOSHE010000054 DAOSHE010000000
VERSION     DAOSHE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_7198_cov_4.443371"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1297)
                     /locus_tag="PLD82_02560"
     CDS             complement(47..1297)
                     /locus_tag="PLD82_02560"
                     /inference="COORDINATES: protein motif:HMM:NF005187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOG64302.1"
                     /translation="MFRVTTGMLKDTMLYNLGVAHREMNIAQNQIATGKKAEFPHQDP
                     SGTINSMLFKSRITELAQFQENIDDGESRLRFYDTALQSTGDILKRLKELAVQAANGT
                     YNDDDRKIAAAEVDQLLKQMIDIANTRYKDETIFSGFRTGELPFKVVYEKVPNSPHAL
                     INRVEYQGDIGIQKREIEQEQYVGVNLPGNRVFWGDEMRISATTPGTDYRATRDQVFR
                     IDGVPVEVKTGDTLAVIVQKINAHHIPVHASIDNTRGANLLVLETTDPHQVWIEDIEG
                     GSVMQDLGVIGRGSNLPPKNFSPTARVSGGSIFDQVIDFRNALLSNDVDALGSMHMGR
                     LEGAIKQITRYQGEVGALDSRLDSVKKRLAMDQVNMTDVLSRTEDIDMAEAITRLRMM
                     ENIERSSMQIGARVIPQTLLDFLR"
     gene            complement(1326..3233)
                     /gene="flgK"
                     /locus_tag="PLD82_02565"
     CDS             complement(1326..3233)
                     /gene="flgK"
                     /locus_tag="PLD82_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOG64303.1"
                     /translation="MISTFHGIEVGKRGLQTHQQGMHVVEHNIANANTEGYSRQRISF
                     EAMQPLYVPGLSREETPGQVGQGNIVQSITRVRNEFIDDRIMEETSTLGYWKTTHEHL
                     YQIEQLHNESSAQTIRNDLDQFWKSWEKLNTNPDDRATRAVVRETAVTLSRSISKSFD
                     RLYGLQANIDTQVQLKVDEINAAARQIADLNAQILKSQQAGDNPNDLMDKRDLVVEKL
                     SRMVNVNVVRGDPDEFMVYVGSQYLVQGQKMRAMVAEGNPQKDGFADVKWADDRSMVK
                     IEKGEIHAFLELRDVTIANQITQIDNMTINLTDLVNETHRDGFGLNFKTGLNFFQEIK
                     LTQNRNGNYDLDGDGQEDHTILFKIAGTKQVDLDAPVGSEGVLNFGPASPDGKPVTVS
                     YTARDRVRDVVERINKSDVGVVAYVNHRNRIAFKAAIPQDNRNQKFTIRHLEDSGDFL
                     VGITGVLRERGAAGAYDWASINQVNKLVGTEKDYSLTPQYHPSRWVDVDVMIKNDLNN
                     IAASQGVDTNGDDRFDKANGPGDGNNALAIARYRYGNGMVGENATFDEFFTSVIGKLG
                     SDSRDAKTHLEKSELVMKHLENNRKSHSGVSYDEEMASMVMFQHGYNASARLIRTLDS
                     MLDTVINRLHA"
     gene            complement(3243..3740)
                     /gene="flgN"
                     /locus_tag="PLD82_02570"
     CDS             complement(3243..3740)
                     /gene="flgN"
                     /locus_tag="PLD82_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOG64304.1"
                     /translation="MYDKETLNRIVEILRDERRTLEEVANLEEKRCHLLTDNYWPRMG
                     ELDEEVSVLIEKLYALEKERLYAGSRMMHTQNADTLGELIRFVSEEDREELVQLQHDM
                     TAVIQRLQFLKSVTEALLKDKKEMVDVTMSAARGESGVTEYTDKGSTREVNTGASSIL
                     FSRRI"
     gene            complement(3817..4044)
                     /locus_tag="PLD82_02575"
     CDS             complement(3817..4044)
                     /locus_tag="PLD82_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64305.1"
                     /translation="MARFGDRFTYCPYCGKRFRYECECPYGKHVKMKLDFDERLKELY
                     RKRFCPWCGSRSTKIECQLCKKSFDSQDMFY"
     gene            complement(4087..4986)
                     /locus_tag="PLD82_02580"
     CDS             complement(4087..4986)
                     /locus_tag="PLD82_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64306.1"
                     /translation="MRIIFGFMAFSILLTACSRTVTEKDESDSYSLVAARWTNAPWLP
                     EVKAAFTAYDARNVEDAFQGLDSAWKNGCRDVLVGYRYAFVLDRRGESERAKMLYLQV
                     SRSLTNEYPDHPYAREVWHNLGHIEYRAGRFEESLSLYRTADRYAQGTNKEIAFSVGM
                     ALRRLKRFSEAIAWFEKADQKDFRTNFYLADVYYEMKKLDLAMQAMQRAVDADPGSSK
                     ALGSLGHYWYALSEQREEESDFSGAQDAIRSSLKLYERAISAGGTNYLEYRKAAKARI
                     SDLERLKAVAKTNGLTRVPLGQE"
     gene            complement(4977..6851)
                     /locus_tag="PLD82_02585"
     CDS             complement(4977..6851)
                     /locus_tag="PLD82_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG64307.1"
                     /translation="MKIVLDGLKKEIAGRVLVDIPSFEFRDNKRTVLVGPNGCGKSTL
                     FGIMAGLVEADAGRVIVDKSRLALLEQDFPAGLSRGTLFDVCADACSHLARREQELHA
                     LEERFSGLDDGARLRYAELREAFERDGGYDWRREVRLVLEGIGFSREEIMKPATSFSG
                     GQKRRALLARALVSEPEVLLLDEPTNHLDLQAIDWLERWLISRKGTTVIISHDRHLID
                     AVADEIVEFRDAGLERYPVPFAAFREERARRNEARFKQWRQQQEFIEKTEDFIRRNIA
                     GQKTKQAQSRRRLLEKIILLEPPPRDTSWKLTLGKAISEGKIVAEARGLSAGYAEQRV
                     LDAVDLTLYRGEKVAIVGTNGSGKSTLIKAFLGMLQAAEGCVRLGENVTIGYFPQEGG
                     ELDPALTVYDEVAKERPTAAEEEIRTLLGAFLFSGDDIYKRISVLSGGEKSRVLLTKL
                     FLRSPNFLIMDEPTNHLDLESRVMLENALADFDGTLLLVSHDRAFVDTIATAVWKIDQ
                     GGRIERLDGNIQDNLAQILGTVSSGREAKPETGDSRITSAPEDGGRKLSRGANRYRIG
                     RIEEEIAELEEKIKIEESKMLSSEALKDGRVYAEIAAECQRLRMELDQRYSAWEEACG
                     "
     gene            complement(6880..>7198)
                     /locus_tag="PLD82_02590"
     CDS             complement(6880..>7198)
                     /locus_tag="PLD82_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017359688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG64308.1"
                     /translation="RGKKQGYAGTAIQVDQKNGKTVFIYEFFPLVRESQPRVAPVERN
                     HTVEPVSETLVDAVEELELRMIRDALLECNSNISQTAASLDIPRQTLQYKMAKYGIRV
                     LPS"
BASE COUNT         1482 a         2065 c         1843 g         1808 t
ORIGIN      
        1 tatgatgaac aaaatcgata cagccaatca accggttatc ccggaattat cgcaaaaagt
       61 ccagcaaggt ctggggaatg acgcgtgcac cgatctgcat cgacgagcgc tcgatattct
      121 ccatcatccg gagacgggtg atggcttcag ccatatcgat gtcctcggtc cgggagagaa
      181 catcggtcat attgacctgg tccatggcga gacgcttttt gaccgaatcg agccgtgaat
      241 caagtgcgcc gacttcaccc tgatagcggg tgatctgttt gatggcccct tccaggcgtc
      301 ccatgtgcat ggagccaagg gcatccacat cgttcgaaag cagggcattc ctgaaatcga
      361 tgacctggtc aaaaatggag ccacccgaga ctctggctgt cggagaaaaa ttcttcggag
      421 gaagattgct cccccgcccg atcacgccca gatcctgcat gacgcttccg ccctctatat
      481 cctctatcca gacctggtgc ggatccgtag tctcgaggac caggaggttc gccccccgtg
      541 tattgtctat cgatgcgtgg accgggatat gatgcgcatt gatcttttgg acaatcacgg
      601 ccagggtatc acccgtcttg acctcgacgg gaaccccatc gatccggaag acctggtccc
      661 gggtagcgcg atagtcggtc ccgggagtgg tggctgagat cctcatctcg tcgccccaga
      721 aaacgcggtt tccgggaaga ttgacaccaa cgtattgctc ctgctcgatc tcgcgcttct
      781 ggattccgat gtcaccctga tattcgaccc ggttgatcaa ggcgtgggga gagttgggaa
      841 ccttctcgta gacaaccttg aagggaagct cccccgtcct gaatcctgaa aaaattgtct
      901 cgtctttata ccgtgtattc gcgatatcga tcatctgctt gagaagctgg tctacctcgg
      961 cagccgctat cttccggtcg tcatcattgt atgttccgtt ggcagcctgg acagctagtt
     1021 ccttgagccg cttgaggatg tcaccagtgg actggagggc cgtatcgtaa aaccgcaacc
     1081 gggattctcc gtcatcgatg ttttcctgga actgcgcaag ctcggttatc cgcgacttga
     1141 agagcatcga attgatcgtc cctgacggat cctgatgggg aaactcggcc ttctttccgg
     1201 tggcaatctg gttctgggcg atattcattt cccgatgggc gacgcccaga ttgtacagca
     1261 ttgtatcctt caacatccct gtcgttacgc gaaacatggt gtaccctccg tgtcaaccat
     1321 taccttcatg catgcagcct gttgatgacc gtgtccaaca tgctgtccag ggtcctgatc
     1381 agtctggccg atgcattgta gccgtgctga aacatgacca tggaggccat ctcctcgtcg
     1441 tagctcacgc cggaatggga tttcctgttg ttttcgaggt gcttcatgac caactcgctc
     1501 ttttcgaggt gtgtcttggc atcccgcgaa tccgaaccga gctttcctat gaccgaggta
     1561 aaaaactcgt caaaggtggc gttttcaccg accataccgt tcccgtagcg ataacgggct
     1621 atagccaagg cattgttacc gtctccgggt ccgttggctt tgtcgaatct gtcatcacca
     1681 ttcgtatcaa caccctgcga ggctgcgata ttgttgagat cattcttgat catcacatcc
     1741 acatccaccc agcgactggg atggtactgc ggtgtcaacg aatagtcttt ttctgttcca
     1801 accagcttgt tcacctggtt gatcgaagcc cagtcatagg caccggctgc tccacgctcc
     1861 ctgagcacac cggtaatccc gaccagaaaa tcccccgagt cttcaagatg ccggatcgtg
     1921 aatttctggt tccggttgtc ctgtggaata gcggctttga acgcaatccg gttgcggtgg
     1981 ttgacatagg ccacaacacc gacatcgctc ttgttgatcc gctcgacaac atcgcggacc
     2041 ctgtctctcg ctgtatagct gaccgtgacc ggctttccat ccggactggc cgggccgaaa
     2101 ttcaggacac cctcgctccc gactggagcg tccaggtcga cctgtttggt tcccgcaatc
     2161 ttgaagagga tggtatggtc ttcctgaccg tctccgtcaa ggtcgtagtt cccgttcctg
     2221 ttttgcgtca gtttgatttc ctggaaaaaa ttcagtccgg tcttgaagtt cagaccgaag
     2281 ccatcccggt gggtttcatt cacgagatcc gtcaggttga tcgtcatgtt gtcgatttgg
     2341 gtgatctggt tggcgatggt cacgtcccgc aactccagga aggcatgaat ctcgcccttc
     2401 tcgattttga ccatgctcct gtcgtccgcc catttgacat cggcaaagcc gtccttctgg
     2461 ggatttcctt cggcgaccat ggcccgcatc ttctgaccct ggaccagata ctggctcccg
     2521 acgtagacca taaactcgtc cgggtcgccc cgcacgacat tcacattgac catcctggaa
     2581 agtttttcaa ccacaagatc ccgtttgtcc atcaggtcgt tcggattgtc tccggcctgc
     2641 tgcgacttga gaatctgggc attcaggtcg gcaatctgtc gggcggcagc attgatttcg
     2701 tcaaccttga gctggacctg ggtatcgata ttggcctgca gaccatagag acggtcaaaa
     2761 ctcttcgaga tgcttcgtga gagagtgact gcggtttccc ggacaacggc ccgagtggcc
     2821 cggtcatcag ggttggtgtt gagtttttcc caggatttcc aaaactggtc caggtcatta
     2881 cggatcgtct gggctgagga ttcgttatgg agctgctcga tctggtagag atgctcgtga
     2941 gtcgttttcc agtatccgag agtagacgtc tcttccatga tccggtcatc gatgaattca
     3001 tttctgaccc gggttatgga ctggacaatg ttcccctggc cgacctgtcc gggcgtttcc
     3061 tcgcgggaga gaccggggac gtagagcggc tgcatcgctt caaaactgat ccgctggcgg
     3121 gaatatccct cggtgttcgc attggcaata ttgtgctcga caacgtgcat tccctgctgg
     3181 tgggtctgaa ggccacgctt tccgacctct atcccgtgaa atgtggatat catcctgacc
     3241 tcctagatcc gccggctgaa gaggatggaa gaggcaccgg tattgacctc tcgagtcgac
     3301 cccttgtcgg tatactctgt cacaccgctc tcgccccgtg cggccgacat cgtgacatca
     3361 accatttcct tcttgtcctt gagcaaagcc tcggtcacgg acttcaagaa ctggagtcgc
     3421 tggatgactg ctgtcatgtc atgttgaagc tggacaagtt cttcccggtc ttcttcggac
     3481 acaaagcgga tcagttcgcc caaggtgtcc gcgttttgag tatgcatcat cctgcttccg
     3541 gcatacaaac gttctttttc gagagcatag agcttctcga taaggaccga gacctcctcg
     3601 tcgagttcac ccatccgggg ccaataattg tccgtcagca ggtggcagcg tttttcttcg
     3661 aggtttgcca cctcttccag tgtgcgtctt tcatccctga ggatctcgac gatcctgttc
     3721 agtgtttcct tgtcgtacac ggcattcccc ttcgagaagg tatctcatgc cgattgtcgt
     3781 catattctgc aaacgggttt attgaaaaac agagtttcaa taaaacatgt cctggctgtc
     3841 aaaggacttt ttacagagct ggcactcgat ttttgtcgat cgggaaccgc accagggaca
     3901 aaagcgtttt ctgtacagtt ccttcaggcg ttcatcaaaa tcgagtttca tcttgacgtg
     3961 tttcccgtag gggcattcgc actcatagcg gaatcgcttt ccgcaatacg gacagtatgt
     4021 aaaacggtct ccaaaacgtg ccatcctgcc tcccggtcat tcatgtcaaa ttcattgagc
     4081 tcaagatcat tcctgcccga gcgggacacg ggtcaggccg ttggttttgg cgactgcctt
     4141 gagtcgttca agatcactga tccgggcttt tgccgccttg cggtattcaa gatagttcgt
     4201 gccgccagca ctgatcgccc gctcatagag tttcagggaa gaccggatgg catcctgggc
     4261 acctgaaaaa tcgctctcct cttcacgctg ttcggaaagc gcataccagt aatgtcccag
     4321 ggacccgagt gccttggatg accccggatc ggcatccaca gcgcgctgca tggcctgcat
     4381 cgccagatcc agttttttca tttcatagta gacatctgcc agatagaaat tcgtcctgaa
     4441 atccttttgg tcagcctttt cgaaccaggc tatcgcctcg gaaaagcgct tcaacctgcg
     4501 caaggccatc ccgacgctga aagcgatctc cttgttggta ccttgtgcgt atcggtctgc
     4561 cgtacggtac agggagagtg attcctcgaa acgtccggca cggtactcga tatgtccgag
     4621 attgtgccag acttcccgcg cgtagggatg atcgggatac tcgttggtca ggctccggga
     4681 cacttgcaaa tagagcattt tagccctctc cgattcaccc cttctgtcca gaacgaaggc
     4741 ataccggtat cctacaagca catcacggca accatttttc catgccgaat ccagaccctg
     4801 aaaggcgtcc tcgacatttc tggcatcata ggccgtgaat gcggccttga cttcgggaag
     4861 ccacggggca tttgtccagc gtgctgcaac cagtgaatac gaatctgatt cgtccttctc
     4921 cgtcactgtc cggctgcatg ccgtcagcaa gatactgaat gccatgaaac cgaaaattat
     4981 ccgcatgctt cctcccatgc actgtaccgt tggtccagtt ccatcctcaa acgctggcat
     5041 tccgcagcaa tctctgcata caccctgcca tccttgaggg cctcgctgga caacattttg
     5101 ctttcttcga ttttgatttt ttcttcgagt tccgcgattt cttcctcgat ccggccgatc
     5161 ctgtagcgat tggcacctct gctgagtttc cgcccaccgt cttcaggagc gctcgtgatg
     5221 cgggaatcgc cggtctccgg ctttgcttcc ctcccggatg agaccgtacc aaggatctgc
     5281 gccagattgt cctggatatt tccatccagt cgctcgatcc ggccaccctg gtcgattttc
     5341 cagaccgctg tcgcaatcgt gtcgacaaaa gcccggtcgt gcgagaccaa gagcagagtt
     5401 ccgtcaaaat cggcaagcgc attctcgagc atcacacggg attcgagatc cagatggttg
     5461 gtcggttcgt ccatgatcag gaaattcgga ctcctcaaaa acagcttggt cagaagaacc
     5521 ctgctctttt cgccaccgga gaggactgat attcttttgt agatgtcgtc cccggaaaag
     5581 agaaatgccc cgaggagggt ccgtatctct tcttcggctg cggtcggccg ttctttggca
     5641 acctcgtcgt agacagtcag tgccggatcg agttcgccac cctcctgcgg aaagtacccg
     5701 atggtgacat tctcgccaag acggacacac ccttcggcag cctgcaacat ccccaggaag
     5761 gccttgatca gggtcgactt cccggaaccg ttggttccga cgatagccac tttttctccc
     5821 cgatagagcg tgaggtcaac agcatccagg acccgctgct ctgcataacc ggcactcaac
     5881 cccctagcct cagccacaat ctttccctcg gagatggcct tgccgagggt gagtttccag
     5941 gacgtgtccc gtggtggtgg ttcgagcaag atgatctttt cgagcaggcg ccgccgggat
     6001 tgggcctgtt ttgttttttg tccggcaatg ttccggcgga tgaaatcctc ggtcttctcg
     6061 atgaattcct gttgctgccg ccattgtttg aagcgggctt cgtttctacg tgcacgttct
     6121 tccctgaaag cggcaaaggg taccggatag cgctcaagac cggcgtcacg gaactcgacg
     6181 atctcgtcag cgaccgcatc gatcagatgc cggtcgtgcg aaatgatgac cgttgttcct
     6241 tttctggaaa tcagccagcg ttcaagccag tcgatggcct gcagatcgag gtggttggtc
     6301 ggttcatcca gaagcaaaac ctcgggttcg gaaaccaggg cccgcgccag aagtgcacgc
     6361 cgtttttgcc cccccgagaa actcgtggcc ggcttcatga tctcctcgcg tgaaaacccg
     6421 attccctcaa ggaccagccg gacctctctc ctccagtcat acccgccgtc acgctcaaat
     6481 gcctcacgga gttcggcgta ccggaggcgg gctccgtcat caagacccga aaaacgttct
     6541 tcaagcgcgt gcaattcctg ttcacgcctc gcaagatggg aacaggcgtc cgcacagacg
     6601 tcgaagaggg ttccccgtga aagaccagcc gggaagtcct gctcaaggag tgccagacgg
     6661 cttttatcca cgatcacgcg gccggcatca gcttccacca gaccggccat gataccgaag
     6721 agggtggatt tgccgcagcc attcggcccg accaatacgg ttctcttgtt gtcccggaat
     6781 tcaaaggatg gaatatcgac caaaacgcgg ccggcaattt cctttttcag tccatcaagc
     6841 acgatcttca tgattcttca aagatacgac gtgaaatcat caagatggta gaacgcgaat
     6901 gccgtatttt gccattttat attgcagcgt ttgacgggga atgtccaggc tggccgcagt
     6961 ctgggaaata ttgctattgc attcaaggag cgcgtccctg atcatcctca gctcaagttc
     7021 ctcgacggca tcgaccagcg tttccgatac gggctcaacg gtatggttcc gttcgaccgg
     7081 cgccacccgg ggctgggatt ccctgaccaa cggaaaaaac tcgtatataa aaacagtttt
     7141 tccgttcttc tggtcaacct ggattgctgt accggcgtag ccctgttttt ttcctctt
//
LOCUS       DAOSHE010000055         7159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9100_length_7159_cov_3.459037, whole genome shotgun sequence.
ACCESSION   DAOSHE010000055 DAOSHE010000000
VERSION     DAOSHE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9100_length_7159_cov_3.459037"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLD82_02595"
     CDS             complement(<1..262)
                     /locus_tag="PLD82_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HOG64309.1"
                     /translation="MAGPSLRRLSMAGRRAAILERVSGGEELMRKALDASTLESLAEL
                     MVECPVGFFGVPLGVATGFVIDGEAVDVPMATEEPSVVAAASH"
     gene            complement(282..1436)
                     /gene="fni"
                     /locus_tag="PLD82_02600"
     CDS             complement(282..1436)
                     /gene="fni"
                     /locus_tag="PLD82_02600"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOG64310.1"
                     /translation="MNTPLESDGARITRRKLRHLDVCADQGLPIEDPRGAGFENIRFI
                     HECLPESDADEIDTRCEFLGYTCRLPILISCMTGGTGDGSPFNVLLARLAQSWGIPIG
                     LGSMRILFHDDGALPDFSIRRYAPDVPILANIGAAQLVPLGVGRIMEMARRLEVDALV
                     VHLNPGQEIVQPGGDRYYRGIMDAIGKLVDLGELPVIVKETGHGIRPRRVRELLGQGV
                     AWVDLAGAGGTDWLQVEACRAQGLGPEESLTATVAETCVSWGLPTALILGALAAGSGV
                     MSERGLPVWLKTREARPVIASGGIKSGLDVARALALGAGLVGMARPFVLAHASGKEAG
                     LERLILETAESLKAAMFLAGAKNCAALRQRRLMVDGGFLAAVGQLAALED"
     gene            complement(1414..2673)
                     /locus_tag="PLD82_02605"
     CDS             complement(1414..2673)
                     /locus_tag="PLD82_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOG64311.1"
                     /translation="MNTANQLPGISDMALYLPASSIESTTVLAARRQEDPENGERLTR
                     ALDYTGQRSIRFTAGYEDSATMAAEAARRLLRQQPEALDGLRFLATGTETGLDHSKPV
                     AAWVQGMLQKDGLRLPGSIVTSQTQHACAGGTISLLGVAAQLALSGRNDERGMVICTD
                     IARYKRSSTAEITQGAGAVAMLVEPEARLVGLDLTSVGHASHDVDDFFRPFGSDVAVV
                     KGRFSIRCYREALEEAFLDHAVRLGEKPATILQGTDIFVLHVPYYSLPLDSLEWLVGR
                     QLGIGRQEAGSFLRERGYQDSILPAAHIGNLYSGSMYMALAFALEGAARRSGDDLPGK
                     RVLFASYGSGNTMMVFRGQVAAGAREVMRSWNTRALLEEHTPADLETYERWMQHYPGE
                     KEGRPPQGAFHLAAVREDGYREYAIGV"
     gene            complement(2670..3326)
                     /locus_tag="PLD82_02610"
     CDS             complement(2670..3326)
                     /locus_tag="PLD82_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOG64312.1"
                     /translation="MDWYGPQGWWPLGPAVRYHKGKYDQPSTGREVFEVYIGAILTQN
                     STWTGACQAMGRLRALRAMTPRALLALPDEVLEEAVRPARYLRQKARYLRAIASFFLL
                     LKGRIPSREELLTVRGVGKETADSILLYAHHQPVFVVDAYTRRVMAALGVIRGDEEYD
                     TIRALFEKELPADEILYNEYHALFVEHARRYYSKKPYGTGDPLITGFPVKRTRQKDTA
                     "
     gene            3609..3890
                     /gene="rpsF"
                     /locus_tag="PLD82_02615"
     CDS             3609..3890
                     /gene="rpsF"
                     /locus_tag="PLD82_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG64313.1"
                     /translation="MQTYEMMCIFDPKLSAEAETTGMVESILKEGGAADVTREDLGLK
                     RLAYLINKKTEGRYFLFHFSLDQQAVAGIRKELGIKEGLLRHLIVRREA"
     gene            3897..4451
                     /gene="ssb"
                     /locus_tag="PLD82_02620"
     CDS             3897..4451
                     /gene="ssb"
                     /locus_tag="PLD82_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG64314.1"
                     /translation="MAFTINHIVLVGGNLTRDAELKYASTGNARLTFSVAMNRSYKDP
                     NGQMKEVAEFFDVVYWGKIAEAVAPRLKKGQKVAIEGRLSQRRYTGQDGQQRNIVEVS
                     ATSVILIGARSDAGPQHDSAYGPGEYEPAPGYAPRAPAQPQTYGQGGQRQGTPWSESQ
                     EIPIDEFPPSGFDDSPMDENDVPF"
     gene            4496..4843
                     /gene="rpsR"
                     /locus_tag="PLD82_02625"
     CDS             4496..4843
                     /gene="rpsR"
                     /locus_tag="PLD82_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG64315.1"
                     /translation="MEERDTAASTNDSGAPQQRYDRGGDRGGDRGERGGDRGGRGDGK
                     KKFFRKKVCFFCSKKIKEIDYKNVELLRRFITEHGKILPRRITGTCAKHQRLLSTEIN
                     RARVVALLPFTDR"
     gene            4872..5420
                     /gene="rplI"
                     /locus_tag="PLD82_02630"
     CDS             4872..5420
                     /gene="rplI"
                     /locus_tag="PLD82_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG64316.1"
                     /translation="MEVILKADVSGLGEEGDIKNVAPGYARNYLLPKGFALPKTATNL
                     KWLDRQKQAILKRKEEKALAARDMAQKISDLSVTVTGKVSSGNRLYGSIHAHEISVAL
                     KEMGVEIDARKIEVGEPIKHLGDFTVTVKLYEGVQSKLKVHVTSVHGDAEEYTARPVA
                     AQTSAAEPVDEAGPDAETQAEG"
     gene            5432..6781
                     /gene="dnaB"
                     /locus_tag="PLD82_02635"
     CDS             5432..6781
                     /gene="dnaB"
                     /locus_tag="PLD82_02635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG64317.1"
                     /translation="METLEVANRVLPHDTGAEQAFLGALIIDPPALDTAITLLAESDF
                     FDVRHRTIFRAIRRLWEARSAIDTITIANFFKDHGLVKDAGGVEYLNEILDATPTAAN
                     VTYYAKIVRDKALLRSLIKAAGEIANTAMRADQDVSSISNYAENLLFEITNRNLTQSY
                     EGIQNIVHASIKIIQDFRNRNGKIGLTTGFSDFDRLTGGLTGGQLIIIAGRPGSGKTA
                     FCLNMASRIAIESKRPILIFSLEMQKEELGVRLLTSEAKVDNSRIKQSLLSQADMNQL
                     IYAADRLYETQIVIDDKPNITVNEIRAKSRRVMSERGDLALIIIDYMQLVDGPEDIPR
                     YDRYNIISDVSRSLKYLAKELNVPVIALSQLSRKIEDRSDRRPMLSDLRESGAIEQDA
                     DIVAFIHRNYIYTKDESEKNIAELLIRKHRNGPQGEIPLHFRGECMRFDDGVRRAEE"
     gene            6778..>7159
                     /locus_tag="PLD82_02640"
     CDS             6778..>7159
                     /locus_tag="PLD82_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64318.1"
                     /translation="MIRFVGAKTLDFVTQLGEISLLLFETVRNTFRRPFPRRNFFEQI
                     VKIGWNSLPVTFFTAISTGAILALQTGYAMMANVKGTEHFVGGVVALSVTRELGPILC
                     AIMIAGRARSGMTAEIGTIKVTEQI"
BASE COUNT         1589 a         2276 c         2013 g         1281 t
ORIGIN      
        1 catgggaggc ggccgccacc accgagggct cctccgtcgc catggggaca tcgaccgctt
       61 ctccgtcgat cacgaacccg gttgccacgc ccagagggac tccgaagaac ccgacgggac
      121 attcgaccat caattcggcc aaggactcga gagtggacgc atcgagggcc ttgcgcatca
      181 gttcttcgcc accggaaaca cgctccaaaa tggcggcgcg ccgcccggcc atgctcaggc
      241 gacgcaggga tggaccggcc atgctacctc caacacgcgc atcaatcttc gagtgcggca
      301 agttgcccga ctgcagccag gaacccgcca tcgaccatca gtctccgctg ccggagtgcc
      361 gcacaatttt tggctcccgc cagaaacatg gcggccttga gactctcggc ggtttcgaga
      421 atcagacgct ccagtccagc ctctttcccc gaggcatggg ccagcacgaa aggccgtgcc
      481 atgccgacca gtccggcccc gagggccagg gcccgggcca catccagccc gcttttgatc
      541 ccgcccgaag cgatcactgg ccgggcctcc cgcgttttga gccagacagg aagaccacgc
      601 tccgacatga cgccactccc ggccgccaga gcacccagaa tcagggcggt cggcagcccc
      661 caggacacgc aggtctccgc aaccgttgcc gtcagggatt cttccggtcc gagcccctga
      721 gcccggcagg cttcaacctg gagccagtcc gttccgccgg ccccggccag atcgacccag
      781 gccacaccct gtcccagaag ctcccggacg cgcctgggcc ggattccgtg ccccgtctcc
      841 ttgacaatga cggggagttc tcccaggtca accagctttc cgatggcgtc catgatcccg
      901 cggtaatacc ggtccccgcc gggctggacg atttcctgtc cgggattgag atggaccaca
      961 agggcgtcca cctcaaggcg acgggccatc tccatgatcc ggcccacgcc taacggaacg
     1021 agttgggctg ctccgatatt ggccaggatc ggaacatccg gcgcgtatct ccggatcgaa
     1081 aaatccggga gggcaccgtc atcatggaaa agaatccgca tcgaaccgag cccgatcggt
     1141 attccccagg actgggccag ccgggccagg agcacattga agggagaacc gtcgcccgtc
     1201 cctccggtca tgcaggaaat caggatgggc aggcgacagg tgtacccgag aaattcgcaa
     1261 cgggtatcga tctcatccgc atcggactcc gggagacact catggatgaa gcggatgttc
     1321 tcaaaaccgg ccccccgggg gtcctcgatg gggagacctt ggtcggcaca gacatcgaga
     1381 tgccgcagct tgcgcctggt gatccgcgct ccgtcagact ccaatggcgt attcacggta
     1441 gccgtcctcc cgcaccgcag cgagatggaa cgcgccctgt ggcggccggc cttccttctc
     1501 cccgggatag tgctgcatcc agcgctcata cgtctcaaga tcggccggag tatgttcctc
     1561 aagaagcgcc ctggtattcc atgaacgcat gacctcccgc gccccggccg caacctgtcc
     1621 gcggaaaacc atcatcgtgt ttccggaacc gtaggaggca aagaggaccc gcttcccggg
     1681 aagatcgtcc ccggaacgac gggctgcgcc ttccagggca aaggccagtg ccatgtacat
     1741 cgaaccggaa tagagattcc cgatatgcgc agcaggaagg atcgaatcct ggtacccgcg
     1801 ttcgcggagg aagctccccg cctcctggcg tccaatcccg agctggcggc cgacaagcca
     1861 ctccagcgag tccaggggaa ggctgtagta gggaacgtgc aacacgaaga tgtcggtccc
     1921 ctggaggatc gtggccggtt tttcaccaag gcggacggca tgatccaaaa aggcttcctc
     1981 gagagcctcc cggtagcaac ggatcgaaaa gcgccccttg acaacggcca catcgcttcc
     2041 aaacggtctg aaaaaatcgt ccacatcatg cgaagcatgc ccgacgcttg tcagatccag
     2101 cccgaccagc cgggcctcgg gttcaacgag catggcgaca gcgccggcac cctgggtgat
     2161 ttcagcggtc gaggagcgtt tgtagcgggc gatatcggta cagatgacca tccctcgttc
     2221 atcgttccgc ccgctcagcg cgagctgggc agcaacgccc aggagggaga tcgtcccgcc
     2281 cgcgcaggca tgctgggtct gcgaggtgac aatcgaaccc ggcaaacgaa gcccgtcctt
     2341 ctgcagcatt ccctggaccc aggcggcgac gggcttggag tgatccagtc cggtttcggt
     2401 ccccgtggcc agaaagcgca gcccgtcgag cgcctcaggc tgctgacgga ggagccgccg
     2461 ggcagcttcg gccgccatag tcgcactgtc ctcgtacccg gcggtaaaac ggatactccg
     2521 ctgtccggta tagtccagag cccgggtcaa ccgctccccg ttttcgggat cttcctgccg
     2581 ccgtgccgca agaacggttg tcgattcgat ggaagacgcg ggaagataca acgccatatc
     2641 actgatcccg ggaagctggt ttgctgtgtt cacgctgtat ccttctgcct tgtccgtttg
     2701 acaggaaatc cggtgatcag gggatctccg gttccatagg gctttttgct gtaatatctg
     2761 cgggcgtgtt cgacgaaaag ggcgtgatac tcattgtaga ggatctcgtc cgcgggaagc
     2821 tccttctcga acagagcccg gatcgtgtcg tactcctcat ccccccggat cacgcccagg
     2881 gccgccatga cccgccttgt ataggcatcg acgacaaaga ccggctggtg atgcgcgtag
     2941 agcaggatcg aatccgccgt ttccttcccg acaccccgta ccgtcaacag ctcttcccgc
     3001 gagggaatgc ggcccttgag caggaggaaa aaagaagcaa tcgctcgcag gtatcgcgct
     3061 ttctgtctca ggtagcgcgc cggtcggaca gcctcctcga gaacctcgtc cggcagggcc
     3121 aggagcgccc ggggtgtcat ggccctgagg gcacgcaatc ggcccatcgc ctgacaggca
     3181 cccgtccagg tactgttttg ggtgaggatg gcaccgatat acacctcgaa cacctcccgc
     3241 ccggtcgacg gctggtcata cttgcccttg tggtaccgga cagccggacc gaggggccac
     3301 cagccctggg gtccgtacca atccatgagc gcatcgaaaa cccgtcgcaa tctgcccatg
     3361 acctagaatg taacaaattt ggaggaccgg ctgtgcaagc tttcgattgt taattatgga
     3421 agaagccatt cgcgcaggat aaaaaccgag gagggggccc ctctcccaga acccgtacct
     3481 gaatcacccg cgggacaaaa ttgtagaatt tgacttacaa cccctctcct ccgtagttta
     3541 tagggatact ctctatcccc ggcacagggc acggggatga atcctatcaa actccacagg
     3601 gaggaataat gcagacgtac gaaatgatgt gcatcttcga cccgaagctc agcgctgaag
     3661 ccgagacaac cggtatggtc gaatccattc tcaaagaggg cggagccgcc gacgtgaccc
     3721 gtgaggatct cggtctcaag cgactggcct acctcatcaa caagaaaacc gagggacgct
     3781 acttcctctt ccacttctct ctggatcaac aggccgttgc cggtatccgc aaggaactcg
     3841 gcatcaagga gggtctcctc cgtcacctga tcgtccggcg cgaggcctga tccggtatgg
     3901 cgttcacgat caaccacatt gttcttgtcg gcggcaacct gacacgggat gccgagctca
     3961 agtatgcctc gaccgggaac gcccgcctga ctttctctgt tgccatgaac cgttcctaca
     4021 aggatcccaa tggccagatg aaagaggtcg ccgaattttt tgatgttgtc tactggggaa
     4081 agatcgccga agctgtggcc ccccgcctga aaaaagggca gaaggtggcc atcgaaggac
     4141 gactctccca gcgacgctac accggacagg atgggcagca acgcaatatc gtcgaggttt
     4201 cggcgacaag cgtgatcctg atcggagccc ggagtgacgc cggtccccag catgattccg
     4261 cctacggacc gggagagtat gaaccggcac caggctacgc cccgcgtgcg cctgcccaac
     4321 cccagaccta cggtcagggc ggacagcgac agggcacccc gtggtccgaa tcgcaggaga
     4381 tcccgattga tgaatttccg ccaagcgggt tcgatgattc gcccatggac gaaaacgacg
     4441 ttccgttctg accggacgga cagacacgaa cacatcacaa ggagataacc atactatgga
     4501 agaacgcgat acagcagcaa gcacaaatga ttccggagcg ccacagcagc gctatgaccg
     4561 cggcggagac cgcggcggag accgtggaga acgcggtgga gatcgcggcg gccgtggtga
     4621 cggaaagaag aaattcttcc gcaagaaggt ctgcttcttc tgctccaaga agatcaagga
     4681 aatcgattac aaaaacgttg aactcctgcg ccgattcatc accgagcatg gaaagatcct
     4741 gccccgccgg atcaccggga cctgcgccaa gcaccagcgc ctgctctcga cagagatcaa
     4801 ccgcgcacgt gtggttgccc tcctcccctt taccgaccgc taatttccca ctaccgcgaa
     4861 ggagcaatta aatggaagtt attctcaagg ccgatgtcag tggactcggc gaagaaggcg
     4921 atatcaagaa tgtggctccc ggctatgccc ggaactacct tctgcccaag ggttttgccc
     4981 tgcccaagac cgcaaccaac ctcaaatggc tggaccgtca gaagcaggcc atcctcaagc
     5041 gcaaggaaga gaaggctctt gcagcccgtg acatggcaca aaagatctcc gatctctctg
     5101 tcaccgtgac cggaaaggtt tcatccggaa accgcctcta tggctccatc cacgcccatg
     5161 aaatctcggt cgctctcaaa gagatgggcg tcgagatcga tgcacgcaag atcgaagtcg
     5221 gcgagccgat caagcacctc ggggatttta cagtcaccgt caaactctat gaaggtgtcc
     5281 agtccaagct caaggttcac gtgacctcgg tgcacggtga tgccgaagaa tacacggcac
     5341 gccccgttgc tgcccagacg tctgcagccg agccggttga tgaagccggt cctgatgcag
     5401 aaacccaggc cgaaggctga gtacagattc aatggaaaca ctggaagtcg cgaaccgtgt
     5461 tcttcctcat gatacgggcg ccgagcaagc ttttctcggc gccctgatta ttgatccgcc
     5521 ggcgctagat accgccatca ccctcctcgc agaatcagac ttttttgacg tccggcaccg
     5581 gaccatcttc cgcgccatcc gccgtctctg ggaggcacgg agtgcaatcg acaccatcac
     5641 gattgccaat ttcttcaaag accacggtct ggtcaaggat gccggcggtg tcgagtacct
     5701 caacgaaatc ctggacgcga cgccaactgc ggccaatgtc acgtattatg caaagatcgt
     5761 ccgggacaag gcccttctgc ggagcctgat caaggctgcc ggggagatcg ccaataccgc
     5821 gatgcgggcg gatcaggatg tctcgagcat atccaactat gccgaaaacc tcctcttcga
     5881 gatcacaaac cgcaacctga cacagtccta cgaaggtatc cagaatattg tccacgcgag
     5941 catcaagatc atccaggatt tccgcaaccg gaatggcaag atcggcctga cgacaggttt
     6001 ttcggatttt gaccgtctga ccggaggtct gaccggagga cagctgatca tcatcgccgg
     6061 acgcccgggt tccggaaaga cggccttctg cctgaacatg gcaagccgga ttgccatcga
     6121 gtccaaacgt ccgatcctga tcttctccct tgaaatgcag aaggaagaac tcggtgtgcg
     6181 ccttttgacc tcggaggcca aggtcgacaa ctcgaggatc aaacagagcc tgctctccca
     6241 agcggacatg aaccagttga tctatgccgc cgacagactc tacgagaccc agatcgtgat
     6301 cgacgacaag ccgaacatca cggtcaacga aatccgcgcc aaatcccgcc gggtcatgag
     6361 tgagcgcggg gatctggcac tgatcatcat cgactacatg cagctcgtgg acggtccgga
     6421 ggacatcccg cgctatgaca gatacaacat catctcggac gtctcccggt ccctcaagta
     6481 cctggccaag gaattgaacg tcccggtcat cgccctctcc cagctctccc gcaaaatcga
     6541 ggaccgttcc gaccgccggc cgatgctctc cgacctgcgt gaatccggag ccatcgagca
     6601 ggacgccgac atcgtcgcct ttatccaccg gaactacatc tacaccaagg atgaatccga
     6661 aaaaaacatc gccgaactcc tgatccgcaa acaccggaac ggcccacagg gcgagatccc
     6721 cctccacttc cgcggagagt gcatgcgctt tgacgacggc gtgcggaggg ccgaggaatg
     6781 atccggtttg tcggtgccaa gactctcgac tttgtgaccc agctcgggga aatctccctg
     6841 ctcctgttcg aaacggtccg gaacaccttc cgccgtcctt ttccacgccg gaattttttc
     6901 gagcagatcg tcaagatcgg atggaattcc ctcccggtca cattttttac cgccatctcg
     6961 accggtgcga ttctggccct ccagacaggg tacgccatga tggccaacgt caaagggacg
     7021 gaacacttcg tgggcggtgt ggtcgccctc tcggtgaccc gtgaactggg accgatcctc
     7081 tgtgcgatca tgatcgcagg ccgtgctagg agcgggatga ccgccgagat cgggacaata
     7141 aaggtcaccg agcagatcg
//
LOCUS       DAOSHE010000056         7085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9237_length_7085_cov_3.625320, whole genome shotgun sequence.
ACCESSION   DAOSHE010000056 DAOSHE010000000
VERSION     DAOSHE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9237_length_7085_cov_3.625320"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..580
                     /locus_tag="PLD82_02645"
     CDS             50..580
                     /locus_tag="PLD82_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG64319.1"
                     /translation="MRGTIGKRNIKLSLIILAPLLVLGIVLLSLPKPAIRTTGKVQYT
                     ASSAKNAETGSAKAFDGDPKTAWVPAKTSDPRFESIQASWSSPVLVSGVRLINGYAGD
                     KNAYSYGAKLRTARILLSDYSSYYWTLKEDEPGMQDISFSQTHDITWIKIFIHSVYKG
                     TSEPPEEVGIAEIEFF"
     gene            complement(771..1298)
                     /locus_tag="PLD82_02650"
     CDS             complement(771..1298)
                     /locus_tag="PLD82_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64320.1"
                     /translation="MAVRKMIVDENHILFNTHGMYYKEFQSDLRQVRFYTVFIVQRAP
                     EKFKEVNLLEQQLSELIKNAIKHGNKNDPNKKVKVWYDFNKRAKIIIEDEGEGFQQLE
                     EWIKFNEERNRCFEEHDFENMEKFVSYRSKHSTEDDGGNALFAALEYWNGGMVWNAKK
                     NKVVVVRYFSEEGES"
     gene            complement(1398..3503)
                     /gene="fusA"
                     /locus_tag="PLD82_02655"
     CDS             complement(1398..3503)
                     /gene="fusA"
                     /locus_tag="PLD82_02655"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG64321.1"
                     /translation="MKEYGTENIRNVAIIGHTGVGKTMLIENALYQAGQIQTLGSIEQ
                     GNTVCDFDEEAIKRKISVQMALATCEFKGNKINFIDTPGSPDFTGDTRASMRVADGAV
                     VLLDAVAGIEIGTETLWHYADDYQVPRIVCVNKMDKEHANFLGVLASLQEKFHKPCVA
                     IQLPIGEGPAFKGIVDLIHMQAVYPDGTGHGVKREAIPAEMQADAEAWRSKLVDAAAE
                     SDDALIEKVLDGKTLTDEEIIAGIRTCVLTYKFIPVGCCSLSVKVGLQLLMNEIIDSL
                     PSPAYRGEYIGKDPDTGAEVVRHPKADEPFAGFVFKTYTDQYAGRICLVRVRAGVLAK
                     DAEILNPRTGTKFRASHLYSMDGKKQIEINTGWTGDILALVKVEGVMCGDTLSDKDKK
                     YIAPPMVLPSPVYFLAIHAKQKKDEEKLNSMLIKAAEDDPTFHVKFNAETRETVIEAM
                     GSQQVDVVLSRIMSRTKIEVETTVPKVAYRETITKPAQGHHKHKKQSGGHGQYGEVYI
                     EVKPIDPSENFSFEDAIVGGAIPRNFIPGVEKGLHEGMAAGVLAAYPMTGVHVKLYDG
                     TFHDVDSSEMSFKIAGRMALRDAVAKAGPVLLEPVMDLTVFVGEDAYGSVISDLNSRR
                     GRLMGSDEGDDGAAAVKKVRAKVPLAELLKYAADLKSLTSGKATFEMSFSHYDPLTGR
                     AAEQIVASRKAELEALDKE"
     gene            complement(3543..4220)
                     /locus_tag="PLD82_02660"
     CDS             complement(3543..4220)
                     /locus_tag="PLD82_02660"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOG64322.1"
                     /translation="MAQNNQDGRRMGKKGIPEIVIERLPRYYRCFRALRGKREKISSQ
                     ELAAEMHTSASQIRLDLSHFGGFGLQGYGYNVEELHARLGEILGVDQTQYYVIAGAGR
                     LGRAIANFPVFQEVGFTLAGIFDIDDAVIGQDIGGVRVSPVEGLAAFLAEHPVRIGII
                     TTPAEAAPGVADVFRAAGVGGILNFAPVDIAPTGNMPVTNIHLTDRLLSLSWRIMNRE
                     RGQDDTR"
     gene            4309..4647
                     /locus_tag="PLD82_02665"
     CDS             4309..4647
                     /locus_tag="PLD82_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64323.1"
                     /translation="MSKETAAINETHLLTIRDLALGGLVGFIIGFVLKKALKIGLLLL
                     AAFVAWQLFTYGTIAPEHMATAKGLQEGATNLLGKHADDIASVKKLFQLNTELAIGFF
                     AGLAIGLWKG"
     gene            complement(4661..6079)
                     /locus_tag="PLD82_02670"
     CDS             complement(4661..6079)
                     /locus_tag="PLD82_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64324.1"
                     /translation="MKKAMVALCFCLHSLSLFSDQGPAMILDDWQGMNLAPRKKVEYY
                     HHPRIRLVRERVEITLRKNEYSVKVHYWFFNNGPEEVLTVAFPFILPVRNFQAIDTWF
                     PVFDVRTIQQTHHTGLCQFMGNWILNGPIPEGNVSDIGMKLLSDRRGSDDREYMRRYY
                     KNIPNANYKYYTVRFPENKEKYMMNSYSSDYGVIGVGKLRGLTANYILETGRFWVEPV
                     ESCDVEVRFEGKKFADLQPQIADLSDHGIGSHSLRPGFSPEGYSIRGDVVRWHWRNVS
                     LDRKRNIMIVLARRSTEALPSVTSSSFLVPPVFVSGEKNQAGESRMDFVNTLGCQSIK
                     WGAPAHWFCDRYKFGMAYYSPYRLLDTSRSVCWAEGVPGPGIGEWVEVDFGSARWVKR
                     FGIVNGLGINMDQYLANNRVKRAEAQFDNGTRRTIEFTDKMLDRQFFSVGAMVRKMRL
                     KILDIYPGSRDDDTCLQNLVVE"
     gene            complement(6124..>7085)
                     /gene="trpS"
                     /locus_tag="PLD82_02675"
     CDS             complement(6124..>7085)
                     /gene="trpS"
                     /locus_tag="PLD82_02675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG64325.1"
                     /translation="PSGDLHLGNWLGAIKNWVSLQDEYECVYCVVDYHAITIPYESED
                     LKRRTTNAVVDLLACGVDPDKSKLFVQSHVPQHTELAWVLACFTSLGDLERMTQFKDK
                     SSQHSQYVNAGLFTYPILQAADIAIYRADAVPVGEDQLQHLELAREIVRRFNGRMGQG
                     HDILPEAQAIVRAGARVMGLDGDAKMSKSKNNYIGIMEEKDAIWKKLGPAKTDEKRLR
                     KTDPGNPEDCNIWSLHNLFADSEATLDWVRAGCTGASIGCVDCKRRLLENMDAEIAPI
                     RERRKDFAADMPRVHKILADSAAWCREEAEITMREVRQALGVR"
BASE COUNT         1567 a         2123 c         1745 g         1650 t
ORIGIN      
        1 gccttcccca aggcagtata ttttcccaat ctttctgggg agaacatgca tgcgaggaac
       61 catcggaaaa cgtaatatca aattgtcgct cattattctc gccccactgc ttgtcttggg
      121 gattgtcctc ctctcactcc ccaagccggc catccgtaca accggcaagg tccagtacac
      181 ggcatcctcg gcaaagaatg ccgaaacagg ctcggcaaaa gcctttgacg gggatcccaa
      241 gaccgcatgg gtccctgcca aaacaagcga cccccgcttc gaatccatcc aggcgagctg
      301 gtccagcccg gttcttgttt ccggtgtgcg ccttatcaac ggctatgccg gcgacaaaaa
      361 tgcctatagt tacggagcaa agcttcggac tgccaggatc ctcctctcag attactccag
      421 ctattactgg accctgaagg aagacgaacc gggcatgcag gatatttctt tttcacaaac
      481 acacgatatt acatggatca agatcttcat ccactccgtc tacaaaggca cgagcgaacc
      541 accggaagag gtcgggatcg cggagatcga gttcttctga tccggtacgc acagactgct
      601 ccgtttggcc ttcggccgtc atcgttgccc gttgaatgct gtttttggtg aacaacctcg
      661 ggccaaaaca ggttgccgtc atccagcggc agtttcccgt cattcccctg agcagaaaaa
      721 accgcccggc gccgggcggt tttttctgct caggggctct actcgtcctc tcaggactcg
      781 ccctcttcgg aaaaatagcg gacaacaaca accttgttct tcttggcgtt ccacaccatg
      841 ccgccattcc agtactcaag cgccgcgaac agcgcgtttc ctccgtcatc ttcggtcgag
      901 tgttttgacc ggtaggagac aaatttttcc atgttttcga aatcatgttc ctcgaaacag
      961 cggttccgct cctcattgaa cttgatccac tcctcgagct gctggaatcc ttccccctca
     1021 tcctcgatga tgatcttggc ccgcttgttg aaatcatacc agaccttgac ctttttattg
     1081 gggtcattct tgtttccgtg tttgatggcg tttttgatca gctcggacaa ctgctgctcg
     1141 aggagattga cctctttgaa cttttcgggc gccctctgca caatgaagac cgtatagaac
     1201 ctgacctgcc tgaggtcact ctggaactct ttgtaataca tgccgtgggt gttgaacaga
     1261 atatgatttt catccacgat catcttgcga acagccattc ctgtcctcct gtttcgaaca
     1321 tgccctctcg tctgccgggc ctgatgtccc cctaaaaata gcagggccgc aagccgggag
     1381 caattccctt gtcttcccta ttccttgtcg agtgcctcga gttccgcctt gcgcgaggca
     1441 acaatctgct cggcggcccg gcctgtcaag ggatcataat gggaaaacga catctcgaag
     1501 gtggccttgc cgctggtcaa cgacttgaga tcagcagcat acttgagcaa ctcggccagc
     1561 ggaaccttgg cccgcacctt cttgaccgct gcagcgccgt catcaccctc gtcggaaccc
     1621 atcaagcggc cgcggcgtga attgagatcg ctgatcacac tcccgtaggc atcttcaccg
     1681 acaaaaaccg tcagatccat caccggctca agcaggacag gcccggcctt ggccacggca
     1741 tcccgcaaag ccatccgtcc ggcgatcttg aaggacattt cggaagaatc gacatcatgg
     1801 aaggtcccgt catagagctt gacatggacc ccggtcatcg ggtacgccgc aagcacgccg
     1861 gccgccatcc cttcgtgcag tcctttttcg acacccggaa taaagttcct gggaatcgcc
     1921 ccgcccacaa tcgcgtcctc aaacgaaaaa ttctctgacg ggtcgatcgg cttgacttcg
     1981 atatagacct cgccgtactg accatgcccg ccggattgtt ttttgtgctt gtggtgcccc
     2041 tgtgccggct ttgtgatcgt ctcccgatag gccaccttgg gaaccgtcgt ctccacctcg
     2101 atcttcgtcc gggacataat tcgtgaaagg accacgtcca cctgctgcga ccccatcgct
     2161 tcgatcacgg tttccctcgt ctcggcattg aatttgacat ggaaagtcgg atcatcttcg
     2221 gcagccttga tcagcatcga gttgagcttc tcttcgtcct ttttctgctt ggcatgaatg
     2281 gccaggaaat agaccgggct gggcaggacc atcggggggg caatgtactt tttgtcttta
     2341 tcgctgagcg tgtccccgca catcacccct tcaaccttga cgagagccag aatatctccg
     2401 gtccagccgg tattgatttc aatctgtttt ttcccgtcca tggaatagag gtggctggcc
     2461 cggaatttcg tcccggtccg gggattgaga atctcggcat ccttggccag cacgccggcc
     2521 cgcacccgca ccagacagat ccgtccggcg tattgatcgg tgtaggtttt gaacacgaaa
     2581 ccggcaaagg gttcgtcagc cttgggatgg cggaccactt cggcccccgt atccgggtct
     2641 ttcccgatgt actcaccccg ataggccgga ctgggcagcg aatcgatgat ctcgttcatc
     2701 aaaagctgga gcccgacttt gacggagagg gagcagcatc cgacgggaat gaacttgtag
     2761 gtcaaaacgc aggtccggat tccggcaatg atctcctcgt cggtcagggt tttcccgtcc
     2821 aggaccttct cgatcagggc atcatcgctc tccgcggccg catcgaccag cttggaacgc
     2881 caagcctcgg catcggcctg catctcggca ggaatcgcct cgcgcttgac cccgtgcccc
     2941 gttccatcgg gatacacggc ctgcatgtgg atcagatcca cgatcccctt gaacgccggg
     3001 ccctccccga tcgggagctg gattgcgacg cagggcttgt ggaatttctc ctggagagag
     3061 gcaagcacac ccaaaaaatt ggcgtgttcc ttgtccatct tgttgacaca gacgatccgc
     3121 gggacctgat agtcatcggc atagtgccaa agcgtctccg tcccgatctc gatcccggcc
     3181 accgcgtcga gaaggaccac agccccgtcc gccacgcgca tggaggcccg ggtgtctccg
     3241 gtaaagtccg gcgaaccggg ggtatcgata aaattgatct tgttcccttt gaactcgcag
     3301 gttgccagtg ccatctgcac actgatcttg cgcttgattg cttcctcgtc gaaatcacag
     3361 accgtgtttc cctgctctat cgagccaagt gtctgaatct gtcccgcttg atacagcgcg
     3421 ttttcaatca gcattgtctt cccaacaccg gtatgcccga tgatggccac gttgcggatg
     3481 ttttcggtac cgtactcctt cattgtctac tccttcgttt cgttttgtaa ggaacaaaca
     3541 gcttatcggg tatcatcctg cccccgctcg cggttcatga tcctccacga gagggacaga
     3601 agcctgtcgg tcaggtgtat attggttacc ggcatgttac cagtcggtgc gatatccact
     3661 ggtgcgaaat tgagaatgcc gccgactccg gcagcacgaa aaacatcggc cacccctggg
     3721 gcggcctcgg cgggagtcgt gatgatcccg atccgtactg gatgctcggc gagaaaagcc
     3781 gccagaccct ccacgggaga gacccggacg cctccgatat cctgcccgat gacggcatca
     3841 tcaatgtcaa aaatcccggc caaggtaaag cccacttcct ggaagaccgg aaaattggca
     3901 atcgccctgc ccaaacggcc ggccccggcg atcacgtagt actgggtctg atccacgccg
     3961 agtatctctc ccagcctggc atgcagctcc tcgacattat acccatatcc ctggagtcca
     4021 aaaccgccaa aatgcgagag atccagccgg atctgggatg cggaggtatg catctcggca
     4081 gccagttcct gactcgaaat tttttcccgt tttccccgca gggctcgaaa acagcgataa
     4141 tagcgcggaa ggcgctcgat cacaatctcg ggtatgcctt ttttccccat ccgccgtcca
     4201 tcctggttat tctgcgccac acagcatccc gcaggcacag cttcatccta tcagaatcga
     4261 taccggtttg taaagggagt atcgcaccaa gccgatactt ggtgtgtcat gagcaaagaa
     4321 accgcagcca tcaacgaaac ccacctgctc accatccgcg atctggcact gggcggactg
     4381 gtcggtttta tcatcgggtt tgtcctcaaa aaagccctca aaatcggttt gctcctgctg
     4441 gccgcctttg tggcctggca gctcttcacc tatggaacca tcgcccccga gcatatggca
     4501 acggccaaag gactgcagga aggtgcaacc aacttgcttg gcaaacacgc ggatgatatc
     4561 gcctcggtca aaaaactctt ccagctcaac accgaactgg ccatcggatt ttttgccggt
     4621 ctggccatcg gactctggaa gggatgatgc gtgccaaggc tcactcgaca accagatttt
     4681 gcagacaggt atcatcgtcc cgtgacccgg ggtaaatatc caggattttc agtcgcatct
     4741 tgcggaccat ggctccaacc gaaaaaaact gacgatcaag catcttgtcc gtaaactcaa
     4801 tcgttcgtct tgtcccgtta tcaaattgag cttcagcacg cttgaccctg ttattggcca
     4861 gatactgatc catgttgata cccaaaccat tgactatccc gaagcgttta acccacctgg
     4921 ctgatccaaa gtctacttca acccattccc cgataccagg ccctgggacg ccctccgccc
     4981 aacaaaccga acgggaggta tcaagcaatc gatatggaga ataataggcc atcccgaact
     5041 tgtaccggtc acaaaaccaa tgcgcgggag cgccccactt tatgctttga cacccgagag
     5101 tattcacaaa atccatgcgc gattcgccgg cctggttttt ttctccacta acaaagacag
     5161 gcggcaccaa aaatgatgag gatgtcacag atgggagagc ctctgtcgat cgacgcgcaa
     5221 gaacaatcat gatattccgc ttgcgatcca gactgacatt gcgccaatgc catcgaacga
     5281 catccccacg aatgctatat ccttcaggag aaaaacccgg gcggagagaa tggctgccaa
     5341 tcccgtggtc cgacagatcc gcaatctgtg gttgaagatc ggcaaacttc ttgccttcaa
     5401 aacgaacttc cacatcgcac gactcgacag gctctaccca aaagcgacca gtttcgagta
     5461 tgtaattggc agtcaatccc cgcaatttcc cgaccccgat caccccgtag tctgatgaat
     5521 acgagttcat catgtacttc tctttgtttt ccggaaatcg cacagtatag tatttgtagt
     5581 tcgcattagg tatgttctta tagtacctcc tcatgtattc tcggtcgtca gaaccacgtc
     5641 gatccgatag cagcttcatt ccgatatcac tcacgtttcc ttcgggaatt ggcccgttga
     5701 gtatccaatt ccccatgaat tgacacaacc cggtgtgatg cgtttgctgg atggttcgca
     5761 cgtcaaacac cgggaaccag gtatctatcg cctgaaagtt gcggacaggc agaataaacg
     5821 ggaacgcgac agtcaaaacc tcttccggcc cattgttaaa aaaccagtaa tgaactttca
     5881 cgctgtactc attcttgcgg agtgttattt caacacgttc gcggacgaga cgaatccggg
     5941 ggtgatgata atactcaact tttttccggg gtgccaggtt cattccctgc cagtcatcaa
     6001 gaatcatcgc cgggccttga tccgaaaaaa gcgaaagaga atgtaaacaa aagcacagtg
     6061 cgaccattgc ctttttcaaa gacacaacct gccatccttc gcttctcttc gttatcagaa
     6121 atctcaccgc acgcccaggg cctgacgaac ctctcgcatc gtgatctcgg cctcctcgcg
     6181 gcaccaggct gcggaatcgg caagaatctt gtgaacgcgt ggcatgtcgg ccgcaaaatc
     6241 cttgcgacgc tcgcggatgg gggcaatctc ggcatccatg ttttcaagca ggcggcgttt
     6301 acaatccacg caaccgatgg aggcgccggt gcagccggcc cggacccagt ccaatgtcgc
     6361 ttcggaatcc gcaaagaggt tgtggaggga ccagatgttg cagtcctcgg gattgcccgg
     6421 atctgttttg cgcaatcgtt tctcgtcggt cttggccggt ccgagttttt tccagatcgc
     6481 gtccttttct tccataatgc cgatgtaatt gtttttggat ttgctcatct tggcatcgcc
     6541 gtccagcccc atcacccgtg cacctgcccg gacgatggcc tgcgcttcgg gcaggatatc
     6601 gtgcccctgc cccatccgtc cattgaagcg gcgcacgatt tcacgggcca gttcaagatg
     6661 ctggagctga tcctccccga ccgggaccgc atcggccctg tagatggcga tatccgcggc
     6721 ttgcaggatc ggataggtaa aaagtccggc attgacgtac tgcgaatgct ggctggactt
     6781 gtccttgaac tgggtcatcc gttcgaggtc gcccagggaa gtaaaacagg ccaagaccca
     6841 agcaagctct gtatgttggg gaacatggct ctgcacaaag agtttcgatt tgtcaggatc
     6901 gactccgcag gccagaagat ccaccaccgc gttggtcgtc cgtcgtttga gatcctcgga
     6961 ctcatagggt attgtaatgg catgataatc aaccacacaa tagacacact cgtattcgtc
     7021 ctgcagcgag acccagtttt tgatcgcccc taaccagttt ccgagatgga gatctccact
     7081 gggct
//
LOCUS       DAOSHE010000057         7075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9252_length_7075_cov_5.417236, whole genome shotgun sequence.
ACCESSION   DAOSHE010000057 DAOSHE010000000
VERSION     DAOSHE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9252_length_7075_cov_5.417236"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1577
                     /locus_tag="PLD82_02680"
     CDS             159..1577
                     /locus_tag="PLD82_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG64326.1"
                     /translation="MVTQAVMFTSRGDGSALRERVVLGHPLIARAIISSARAGVKEFF
                     VVCDEGADELAAMLDANKVLAKHDCVVQCLSATKCASDAKLAKKLSAGFLLIPDTAVV
                     KQELVARFAARPPAKGSSLLLLDSDTRSIREYDGIWRVRLDEKSGKLAEAGRDLDAWN
                     ASWTGLALCPGQLLGKIVQEFVKRPEGWYEKSFLKFFTPATTGFTYCAQDYAINVCSP
                     QLAERAEKILLNSVRKATDGFVSRNFNRYVSLFLSRWLIRVGASPNFISGANLLLGVM
                     ASILLGMGGHLEAIIAALLFQFTSIVDGSDGEVAKLTWTMSPNGAWIDTICDQLTYVM
                     FFVALPLGLYFDKTVLTEGGNPLFIVLGGVTLLAFGAMFWLMSRYVKRVRGEGSMLQI
                     IKDIEESAKQPGLGGLVDRMVMKISFIFRRDFFAFATMIVLIAGLSAPLMWLISSLCT
                     IQVGYLWLFSQRRFRMLGLRKR"
     gene            complement(1647..2024)
                     /locus_tag="PLD82_02685"
     CDS             complement(1647..2024)
                     /locus_tag="PLD82_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOG64327.1"
                     /translation="MTNEVATRTTNDQETSRLRRVSMPVDIRETPEAFLLTAEIPGAT
                     RDSVTIELEHDELTIEARADLQGTGRAIISEFSPTIWSRSFHVGRLVDRDQIEARVEN
                     GLLHLRLAKAKDARPQRIEIKTA"
     gene            complement(2074..2484)
                     /locus_tag="PLD82_02690"
     CDS             complement(2074..2484)
                     /locus_tag="PLD82_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG64328.1"
                     /translation="MYSWNILDDMFSMRDLMEKAFGELSGGLSGGTPAINVYESETAS
                     EVKALVPGYTRDELEINYEKGLLTISSREQNEDKKPDGKALREECCRTSFRRSVRFAK
                     EIDRNAISARLENGVLTVILPHQAESTPKKIEVQ"
     gene            3079..4122
                     /locus_tag="PLD82_02695"
     CDS             3079..4122
                     /locus_tag="PLD82_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG64329.1"
                     /translation="MLLLGRIMLPLLLVLAACLPDHSSGRKEGAHMVSDKPVRVGLVL
                     SHFGLGDQSFNDMQYNGLIEAYRRFDIHTVFRVPRSEADTDMRPVFDELIHREQCNVL
                     IAGEGFQMGPLVYELAKKYPDVYFIVFEYKAGVLPNVINAEFAQNEGSYVAGFLAARF
                     SVTKKIAFMGGVDIAVIKDFEAGFRQGMQRGEPSCQLVTEFVSRLPDYSGFYDPKKGY
                     EMASRLYKEGADIIYAVAGGTGNGVIQAAREAGKYVIGVDSNQDHMAPGQVLTSMMKR
                     LDVAVLRLIEMRIAGTLRGGVYRFDYKNGGVSLTEMEYTKKMFSEPLLRELRTLEKQM
                     ADGVITVINTLKG"
     gene            4130..5788
                     /locus_tag="PLD82_02700"
     CDS             4130..5788
                     /locus_tag="PLD82_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64330.1"
                     /translation="MSDTSMTASQETGTKVEMDPLRGMDSGEIRFRLFFKFSIVISLI
                     LVLTMTITGWVLTGIQEKSLIAEKEKSADKLIQFVATISAFHIERFSYFVLHENAEML
                     QSGQGMEADVLSVIVRDAPEDKTKEGKKLHFNGREPSRIDVPNKYWMKKTLPCVYHGG
                     GVNKTVGSVEMIFSLESVFRTIANARLTFILIMLLTIVVIGVAVAYLLMKLVVRPLKV
                     LTDSAALIAKGDFDIVVDLKTRDEIGFLGDSITNMSRELKESFSEIASQKEEIQKYSQ
                     NLEGMVEQRTAELNTANKELTTVNKQLLSELEMARRVQQGIIPSAETFPRRAELSIGS
                     SYLSMASVGGDLYDVVRIGRNSYGFLIADVSGHGVPAALITTMAKVSFSTHSLFGVPP
                     DEICTKVNRDIHRIIADLGYYLTAYYGILNLENGIFQFTNAGHHAALLWRKRDGSIEK
                     LDSPGLFIGISEDGNYETRTATIEAGDRILLFTDGIVEARNPAGDFFEYDRLFEYIET
                     HSELAPTDFVDGLIKTVDAFCEGKPADDDRAVLYVEFVQTMVEN"
     gene            5881..6222
                     /locus_tag="PLD82_02705"
     CDS             5881..6222
                     /locus_tag="PLD82_02705"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOG64331.1"
                     /translation="MESIIDRLYAVIEDRRDNPPPKSYVSSLFAGGVTKIAGKVTEEA
                     AEFVEAAGEEDEKHFVYEAADLMFHVLVMLGHRHVHPQQVFDELARRFGISGIDEKEA
                     RKHGGGYADSQ"
     gene            6206..7072
                     /gene="hisG"
                     /locus_tag="PLD82_02710"
     CDS             6206..7072
                     /gene="hisG"
                     /locus_tag="PLD82_02710"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOG64332.1"
                     /translation="MLTANDELRIAIPSKGRLMEQSIDLLKKSGLKFNASGRQLFARC
                     KDTGVMVIFVNAQDIPALVEEGVVDLGITGSDLVREKKASVTEHMPLGFGRCRLSFAT
                     HVDSSVKTAKDFSGRTLGTKFVSTAQQYFSEQSVKDVRILEINGAVEVMVLLGLVDGI
                     LDIVETGSSLREHDLVERETVLTAQAVLIGNPSPRSSEMRDRLLRRIEGVLVASRWSL
                     LEYNCPGDRIDEAKKITPGYSSPTIQSTDSGTWLAVKVMVEKDRVQSVMDQLEAIGCR
                     AIIETDVVHCRL"
BASE COUNT         1493 a         1791 c         2165 g         1626 t
ORIGIN      
        1 gctgctccga aaaaggccgc tgctccgaaa aaggccgctg ctccgaaaaa ggccgctgct
       61 ccgaaaatca cccgcaagaa aagctgacgc atattcttgt gggggatgca taatggaagt
      121 accatcagag aattggtgaa ggaagcagga ggaagagtat ggtaacacag gccgtcatgt
      181 ttacaagtcg tggtgatggg agcgcgcttc gggagcgggt tgttctcggg catcccctga
      241 ttgccagggc catcatctct tccgcccgtg ccggggtcaa ggagtttttc gttgtttgtg
      301 atgagggtgc ggatgaactg gcggcgatgc tcgatgccaa caaggtcctg gcgaaacacg
      361 attgcgtcgt gcagtgtctg agtgcgacca agtgcgcttc ggatgcaaag ctcgcaaaaa
      421 agttgtcagc cggatttctg ctgatcccgg atacggcggt ggtcaagcag gaattggtgg
      481 ctcggtttgc cgccagaccg cccgccaaag ggagcagcct tcttcttttg gacagcgata
      541 cgcgcagcat acgcgagtat gacgggatct ggcgtgtccg tctcgatgaa aaatcaggga
      601 agctggcgga ggccgggcgg gatctggatg cctggaatgc ctcctggacc gggctcgccc
      661 tttgtccggg ccagctctta ggcaagatag tgcaagagtt tgtcaaacgg ccggaggggt
      721 ggtacgaaaa gagtttcctc aagttcttca cgccggcaac gaccggcttt acctattgtg
      781 cccaggatta tgccatcaat gtctgctcgc cgcaattggc cgagcgtgcc gagaagatcc
      841 tcctgaattc cgtccgcaag gcgacggacg gattcgtttc ccggaatttc aaccgctatg
      901 tttcgctctt tctctcacgc tggctgatca gggtcggggc ctctcccaat ttcatcagtg
      961 gtgccaatct ccttctcggg gtgatggctt cgatccttct tggcatgggc gggcatcttg
     1021 aggcgatcat cgcggctctg ctcttccagt ttacgtcgat tgtcgacggg agcgacggag
     1081 aggtggccaa actgacctgg acgatgtcgc ccaacggggc atggatcgat accatttgcg
     1141 accagctgac ctatgtgatg tttttcgttg cccttccttt gggactgtac ttcgataaaa
     1201 ccgttttaac cgagggtgga aaccccctct ttattgtgct gggcggggtg accctgttgg
     1261 cgtttggcgc gatgttctgg ctgatgtcgc ggtacgtcaa gcgcgtccgc ggagagggga
     1321 gcatgctcca gatcatcaag gatatcgaag aatccgcaaa acaaccgggt ctcggtggcc
     1381 tggttgaccg catggtgatg aagatctcct tcatcttccg gcgtgatttt tttgccttcg
     1441 cgaccatgat tgtcctgata gccggccttt cggcgccgtt gatgtggctg atcagctcgc
     1501 tctgcaccat tcaggtcggg tatctctggt tgttttcgca acggcggttc cggatgctcg
     1561 gtttgcgcaa gcgctgagac ccgcagttgt caaaaagaaa aggccttccg gctgatgcgg
     1621 gaaggccttt tttgttgtgc ggggtctcac gcggtcttga tctcgatccg ttgcggtcgg
     1681 gcgtccttgg ctttggccag tctcagatgg agcaggccat tttcgacacg ggcttcgatc
     1741 tggtcccggt cgacaagtcg accgacatgg aaggagcgtg accagatggt cgggctgaac
     1801 tcgctgatga tggcccgccc ggttccctgg agatcggcgc gcgcctcgat cgtcagttcg
     1861 tcatgttcaa gctcgatcgt cacggagtcg cgggtggctc cggggatttc ggccgtcagc
     1921 aagaaggcct cgggggtttc ccggatatcg accggcatgg agactctgcg gagccgggaa
     1981 gtttcttggt cgtttgtggt gcgggttgcg acttcgttgg tcatggtggg cctcctggtt
     2041 tcggtgtttc ggcggaaaat tggggcgaac atgttactgt acctcgattt tcttgggtgt
     2101 gctctcggcc tgatggggca ggatgacggt caggacgcca ttttcgaggc gtgcgctgat
     2161 ggcattgcgg tcaatctcct tcgcgaagcg gacacttcgc cggaacgagg tgcggcagca
     2221 ttcttcgcgc agggccttgc cgtccggttt tttgtcttca ttctgctccc tggaggagat
     2281 tgtcaagaga cctttctcgt agttgatctc gagttcgtcg cgggtatacc cgggaacgag
     2341 ggccttgacc tcgcttgcgg tttccgactc gtagacgttg atggccggtg ttccgccgga
     2401 gagtccgccc gagagctcgc caaaagcctt ttccatcagg tctctcatgg aaaacatgtc
     2461 gtcgaggatg ttccagctat acattgagtg cctccttggc atcaattttt ttcacagata
     2521 tgtattgcaa caggcgtgcc aagcgtaaaa atttttatct tacgaaaaaa caacaagata
     2581 gaggttgcca gaatgatgcc gcacgcatgt taagtaaaaa tgtgtcaagt cttctcactg
     2641 acctccgcaa gctgatggga aaatgtgtca aaacaacaca gtggattggc acagagtggc
     2701 gaaagcgtgt actggtgaac cagtgatccc gaagaggaga ccaaacaggc ctgaaaaaca
     2761 tacgtgctcc cataacctgc tgcagcccgg tcaagaatac cgggccagcc tcgccggatc
     2821 agtgacaggg tgacggaaaa attcaggacg ccataccctg aaagaaatac ttaaagaggg
     2881 aaagggtctc cgcgaccgga tcaggcccgg gtcggtggac aggggggtct ggctgtcagg
     2941 atgccggctt tgtgcgggat gtgtgccatg gtgctgtccg gtgtgccaaa cgtcgagttt
     3001 gtttgcggga gacgcttcca taagatcttg aagcgtgata cactggcgtg aatgccggat
     3061 tgctctggag gaaaacgcgt gttgttgctt ggtcgcatca tgctcccgtt gcttttggtt
     3121 cttgcagcct gcttgcccga tcattcttcc gggaggaagg aaggggcaca catggtttcg
     3181 gacaagccgg tcagggtcgg gcttgtcctc agccacttcg ggctgggcga ccaatcgttc
     3241 aatgacatgc agtataacgg gctgatcgag gcctacaggc ggttcgatat ccataccgtt
     3301 ttccgggtcc cacggagcga ggcggatacg gatatgcgcc ccgtgttcga cgagttgatc
     3361 catcgcgagc agtgcaacgt cctgatagcc ggtgaggggt ttcagatggg gccgctcgtc
     3421 tacgaactgg ccaaaaaata tcccgatgtc tactttatcg tgtttgagta caaggccggg
     3481 gttctgccaa acgtcatcaa tgccgaattt gcccagaatg aaggctccta cgtggcggga
     3541 ttcctggctg ccaggttctc ggtgaccaaa aagatcgctt ttatgggcgg ggttgatatc
     3601 gctgtgatca aggactttga ggccggattc cgccagggga tgcagagagg tgagccgtct
     3661 tgtcaactcg tgactgaatt tgtctccagg ctcccggact actccggctt ttacgacccg
     3721 aagaaggggt acgagatggc cagccggctc tacaaggaag gcgcggacat catctatgcc
     3781 gtggcgggtg gtacgggaaa tggtgtgatc caggcggcgc gcgaggcggg gaaatatgtc
     3841 atcggtgtgg attccaacca ggatcacatg gcgcccggtc aggttctgac gagcatgatg
     3901 aagcggctgg atgttgccgt tctccggttg atcgagatga ggattgcggg aacactccgg
     3961 ggcggggtgt accgctttga ttacaaaaac ggtggcgtga gcttgacgga gatggaatat
     4021 acaaaaaaaa tgttttccga accgctcttg cgggagttgc gcacacttga aaaacagatg
     4081 gccgacggtg tgattaccgt catcaatacc ctgaaaggtt gatcgaccca tgtccgacac
     4141 cagtatgact gcaagccagg agaccggcac aaaggttgag atggatccac ttcgcgggat
     4201 ggactccggc gaaatacgtt tccggctctt tttcaaattt tccatcgtca tttccctgat
     4261 cctggtcctg accatgacca tcacaggatg ggttctgacc ggcatccagg agaaatcgct
     4321 gatagctgaa aaggaaaaga gcgccgacaa gctgatccag tttgtggcga ccatcagcgc
     4381 tttccatatt gagcgctttt cgtattttgt cctgcatgaa aatgccgaga tgctgcagtc
     4441 ggggcagggg atggaagcgg atgttctctc ggtgatcgta cgggatgctc cggaggacaa
     4501 gacaaaagaa ggcaagaagc tgcacttcaa tggccgcgag ccttccagga tcgatgtgcc
     4561 gaacaaatac tggatgaaaa agacacttcc ttgtgtctat catgggggtg gtgtcaacaa
     4621 gacagtcggc tccgtggaga tgattttttc gctcgagtcc gtcttccgga cgatcgccaa
     4681 tgcccggttg accttcatcc tgatcatgct tttgaccatt gtcgtgatcg gagttgcggt
     4741 tgcctacctc ttgatgaaac tggtggtgcg gcccctcaag gtcctgacag acagcgcagc
     4801 cttgattgcc aagggcgatt tcgatatcgt cgtcgatctc aagacgcggg acgagatcgg
     4861 gtttttgggc gattccatca cgaacatgag ccgtgaactc aaggagagct tttccgagat
     4921 tgcatcgcag aaggaagaaa tccagaagta ctcgcagaac ctcgagggaa tggtcgagca
     4981 gcggacggcc gaattgaata cggccaacaa ggagcttacg actgtcaaca agcagcttct
     5041 ctccgaactc gagatggcgc gacgcgtcca gcaggggatc atccccagtg cggagacctt
     5101 cccccgcagg gctgaactct ccatcgggtc gagctatctc tcgatggcgt cggttggtgg
     5161 agacctctat gacgtggtgc gcatcggacg caattcctac ggctttttga tcgccgatgt
     5221 ctcgggacac ggtgttccgg ctgcgctcat cacgacgatg gccaaggtct cctttagtac
     5281 ccactccctc ttcggggtgc cgccggacga gatctgcacc aaggttaacc gggatatcca
     5341 caggattatt gccgatctcg ggtattacct gacggcctac tacgggattc tcaatctcga
     5401 aaacgggata ttccagttca cgaacgcggg ccatcatgcg gcactcctct ggagaaagag
     5461 ggacgggagc atcgaaaagc tggattctcc gggcctcttt atcgggatca gcgaggacgg
     5521 gaactacgaa accaggacgg cgacgatcga ggccggcgac aggatcctcc tctttacgga
     5581 cgggattgtc gaagcaagaa atccggccgg cgactttttc gagtatgacc ggctcttcga
     5641 gtatatcgag acccatagtg agcttgcgcc gaccgatttt gtcgatggcc tgatcaagac
     5701 cgtcgatgcc ttttgtgaag ggaagccggc cgacgatgac cgggcggtcc tttatgtcga
     5761 gtttgtccag accatggtcg agaattaacg gacagggcca ccctcctctt ttgtaccttt
     5821 acgggacaag gatttccgcc cggggcggaa atccgtctgt gaggaaaggg ctctctatca
     5881 gtggaaagca tcattgacag gttgtacgcg gttatcgagg atcgcaggga taatccgcct
     5941 ccaaaaagct atgtctcgag tctctttgcc ggtggtgtca cgaagattgc ggggaaggtg
     6001 accgaggaag cggcagagtt tgtcgaggct gcgggtgaag aagacgagaa acacttcgtc
     6061 tatgaagctg cggatctcat gttccacgtt ctggtcatgc tcggacaccg gcatgtccat
     6121 ccgcagcagg tatttgatga actggcccgt cgtttcggga tcagcgggat cgacgaaaag
     6181 gaagcacgca agcatggagg tggatatgct gacagccaat gatgaattgc ggattgccat
     6241 accctccaaa ggccggttga tggagcagtc catcgatctt ttgaagaaat cgggtctcaa
     6301 attcaatgcg agcggtcgcc agctctttgc gcggtgcaag gataccggtg tcatggtgat
     6361 ttttgtgaat gcccaggata tcccggccct tgtcgaggaa ggcgtcgtcg atctggggat
     6421 caccgggagc gacctggtcc gggaaaagaa agccagcgtg accgaacaca tgcctttggg
     6481 tttcggtcgc tgccggctct cctttgctac ccatgtggat tcttccgtca agacggccaa
     6541 ggacttttca ggacgcacac tggggacgaa atttgtcagt accgctcaac agtatttttc
     6601 ggagcaaagc gtcaaggacg tgcggatcct cgagatcaat ggtgcggtcg aagtcatggt
     6661 gctcttgggt ctcgtggacg ggattctcga tattgttgag accgggagca gcctgagaga
     6721 acacgatctt gtcgagcggg agactgtttt gacggcccag gctgtcctga tcgggaaccc
     6781 ttcgccccgg agcagcgaga tgcgggacag actcctgcgc aggatcgagg gcgtgcttgt
     6841 ggcatcccgc tggtccctgc tggagtacaa ctgccccggt gacaggattg atgaagccaa
     6901 gaagatcacg ccgggatatt cgtccccgac catccaatcg accgacagcg ggacctggct
     6961 ggccgtcaag gtgatggtgg agaaggaccg ggtgcagagc gtcatggatc agcttgaagc
     7021 gatcggttgc cgggccatca tcgagacgga tgtcgtgcac tgccgcctct gagcc
//
LOCUS       DAOSHE010000058         7059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9279_length_7059_cov_4.118218, whole genome shotgun sequence.
ACCESSION   DAOSHE010000058 DAOSHE010000000
VERSION     DAOSHE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_7059_cov_4.118218"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..640
                     /locus_tag="PLD82_02715"
     CDS             110..640
                     /locus_tag="PLD82_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64333.1"
                     /translation="MRRVLTAISVLFAVSTSGYAFATDAGSWSFAGEISLETGYGAAW
                     DSMRISGSPSLSWFFADCFAVKGSLWFERQEIGKETAERGGGYLGVDWYFRGPASPVF
                     PFVGVSVGGGRFAWGETSVGYAGLRVDAGMVFLFNETIGIRLLASWFRDTVDTQGRDA
                     TGGRLLLGAGVHLFLF"
     gene            complement(753..1652)
                     /locus_tag="PLD82_02720"
     CDS             complement(753..1652)
                     /locus_tag="PLD82_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG64334.1"
                     /translation="MTTPCITAIQQKGPAHLGILASGPGLDVYSRLKGSITLHDRTSK
                     GILAQLRNEAYTMVFVLKDDFASSLLAFRCRIRTRIGIAREFSSLLFTQAFKPAMLPR
                     HGAARYFPLLGMKPPENLALRFALSEQELSRAKELLAAVSAPYIVLAPTTTRPEKDWP
                     VEHCRSLIQLLSRNEHPVVIVGSARDTLRHRLITAETCATDLTGLTTTGEAAAIIGNA
                     SGVVACDSGPMHIAAALEVPLVALFGQTDPARSGPCSPHAVILRAKTACAPCLDRHCG
                     PTAPCMASISPEQVYSTIFRILP"
     gene            1966..2493
                     /locus_tag="PLD82_02725"
     CDS             1966..2493
                     /locus_tag="PLD82_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64335.1"
                     /translation="MEDLRKRVKDERSFLEKAGKLIPGYSGYKEKLIRQEGDKLLRDW
                     LAGKMQTEMGYLKDLIDEMTRSMKIKLLDHVDRPLKKLEKLRDRIKFADRGYTGWFAA
                     IDVDKDLLDRMYEFDNSLIDLVTEIEQLIRSAQAAVSDDDKLVSALKSLTDLIAQADV
                     NYNERENLILNKAEG"
     gene            2500..3534
                     /locus_tag="PLD82_02730"
     CDS             2500..3534
                     /locus_tag="PLD82_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG64336.1"
                     /translation="MALTSGVLEYFDESGNVMAQRLPEAGSGEFKMGTYVVVRESQEA
                     VFFRDGQMLDTLGPGRHQITTANLPLITKLFSLVTTGGESPFKAELYFVSKQPFLNEG
                     WGTDQPILFRDKEFGMVRLRAFGICSHRIIDNNLFINKVVGTRGRYARDDAKDFIRNI
                     IVGRLNDALGEVMETILDLPKYYDEVGVAMKSKVRDDLEKYGIEIVDFIIKAITPPEE
                     VQKAIDERTSMGAIGNMQQYMMFKAAKSMQDAANNQGGGAGMGMGMGAGIGMGMMMPG
                     MMAGAFMPGMQQPMQPGMQQPMQPGMQQPVQSAQPEAPAAGGTFCTNCGTQNPAGAKF
                     CGNCGNKMGG"
     gene            3707..5113
                     /locus_tag="PLD82_02735"
     CDS             3707..5113
                     /locus_tag="PLD82_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64337.1"
                     /translation="MVRVSAASSGMRLVCMLALFFFSMLALLGGFIGGVLLSSGTWHE
                     GDMAALVEERGIGSLFTLNDPDLAVFHLAAERLLAQSPVLAVDVLSRDAILYSKAIQS
                     NAGERALPANSALIRQMGRIICHDGRLLYACQPSFAREILVVLEVTQEDRPWRATLSR
                     AIARVGEWPGWGIPVIIAGWSLLSLTLAWCIVWMLYSRPVSRVVAVLEACEQVEDLAT
                     AKLFGGTVSDPLVGCIERLVQRAGGGKKTGFESLLPRRRGYDFPGFDAATVYAGTSEK
                     LAVHVDIRPLGDGRYGIMLLAVRAEDESEEALVRIATVCDACFSLYVNPAQVASSLNR
                     YMHSMSIPFPVDAGFLVWSPSEKKLEFCLNGSVNLFRYDVREGTLRGYDLKQPVLEGG
                     LPEEFDAGIGFARLDIGEGDPVVLAYAPGVEWESLVTDVLKKSTTLAERQQTLNALLG
                     QRLGPGDAAAVLMMSLSE"
     gene            5110..5952
                     /locus_tag="PLD82_02740"
     CDS             5110..5952
                     /locus_tag="PLD82_02740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG64338.1"
                     /translation="MKRFLVPVVLVYSMVLCPVMGLAAQEGSKPVFRVPVLCYHAVSP
                     VAAGRFAVTPEVFSMHMEYLKKNGHTVISVAGLVSFLKAVQKGEKVSLPEKPVVITFD
                     DNYESIQKHALPVLTKYKYPACNFIYLDGISVAGWERYKRLEGSLMELHSHTVNHVDL
                     AKKKQDEDDKSWRKRIFREIADSRRIIGERLGRSPAFIAYPYGTWNVSVIRMMRIAGY
                     SAGFSAFGGYVRETSSLDVLPRFTIFREYDLQKFAEIVSGAWVGGQGEPDFISARDYN
                     FRIE"
     gene            6221..>7059
                     /locus_tag="PLD82_02745"
     CDS             6221..>7059
                     /locus_tag="PLD82_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG64339.1"
                     /translation="MGRKEITGRIVNPASGEIRSGRIVFEDGRILEMLADEDVPERYI
                     VPGFVDSHIHIESSMLMPSRFAAAALRHGTLAAVCDPHEIANVLGLEGVRGFLADAEN
                     APFSFVFGAPSCVPATSLETSGAALTACDIKTLFSEDGLRFLGEVMNVPGVLAGEKEL
                     EAKLDLAKEYGLPIDGHAPGLLGADLRSYVAAGMTTDHECSTVAEAMEKVRQGMFVQI
                     RDGSAARNFDDLLPLFFQVPGRLMLASDDKHPDDLLGGHINRMVSRALKAGVPFGAVL
                     RAA"
BASE COUNT         1496 a         1664 c         2176 g         1723 t
ORIGIN      
        1 gggaatgaga tcacctgatc atggccgaaa aacaggaatc cggatttttc cggcgtatcc
       61 ccatgccagg agattgtttc tttttccatc tggtgctatc atctccggta tgagaagagt
      121 gctgacagca atttctgtct tgttcgcagt ttcgacatcg gggtacgcct ttgcgacaga
      181 tgccggatca tggtcgtttg caggcgagat ttcgctcgaa accgggtatg gtgcggcttg
      241 ggacagtatg cgaatttcag gcagcccatc actttcctgg ttttttgccg actgttttgc
      301 ggtcaagggt tcgctctggt ttgaaaggca ggagattggc aaagaaactg ctgaaagggg
      361 cgggggatac ctgggagtgg attggtattt ccgtgggcct gcatcaccgg tctttccttt
      421 tgttggcgtt tctgtggggg gcgggcgatt tgcctggggc gagacgagcg ttggatatgc
      481 cggactgcgc gtggatgccg gtatggtgtt tttgttcaat gaaaccattg ggatcaggct
      541 gcttgcaagt tggttccggg atacagtcga tacacaaggt cgggatgcga caggtggaag
      601 gcttctgctt ggtgccgggg tccatctttt ccttttttga ccgtgcctga agcgatttat
      661 ctggatgtct ttgttgttgc tgttttttga tgaacagaag acctgccggg cgggaagcgg
      721 attgtcctga tttgatcctt gtaccctgca ggctatggca gaattcggaa aatcgtcgag
      781 tacacctgtt ccggactgat ggatgccatg catggtgcgg ttggaccgca gtgccggtcg
      841 agacagggtg cgcaggccgt ttttgcccga aggatgaccg cgtgtggtga acaggggccg
      901 gaacgggccg gatcggtctg gccaaaaagt gccacgaggg gtacctccag agcggctgcg
      961 atgtgcatcg ggccactgtc acatgcgacc acaccggatg cgttgccgat gatggcggct
     1021 gcttcgcctg tggtcgtcaa tcccgtgaga tccgtggcac aggtttccgc tgtgatcagc
     1081 ctgtgcctga gtgtatcccg tgccgagccc acaatgacaa cggggtgttc attccgggat
     1141 aaaagctgga tcaaggacct gcaatgctca acaggccagt ctttctccgg tcgtgttgtt
     1201 gtcggggcca ggacgatata tggtgcagaa acagcagcga gaagttcttt ggcgcgagac
     1261 agctcttgct cggagagtgc aaagcgcaag gccaggtttt cggggggctt cattcccagc
     1321 aatggaaagt atcgtgctgc accatggcgc ggaagcattg caggtttgaa cgcttgtgtg
     1381 aataataatg aagaaaactc acgggcaatg ccgatccgcg ttcgaatccg gcaccggaat
     1441 gccaacaagg acgatgcaaa atcgtctttc aagacgaaga ccatggtgta ggcttcgttg
     1501 cggagctgtg ccaggatccc cttgcttgtc cggtcatgaa gcgtgatcga tcctttcagg
     1561 cgggaataga catcaagacc gggtccggac gccaggattc caaggtgcgc aggtcctttt
     1621 tgctggatgg cagtaatgca aggcgttgtc atcagggcat cacccaaaaa cgaatggcct
     1681 atgacgagaa tacgctcatt gctgagtggg ccggtttcca aggatctgaa gcgggcatgc
     1741 atatgttgaa tatacatgcg ggatacgagg aatgcacccg tttcgccctg attggttgac
     1801 tggggagggt gcatggccgg tggccccggg tggttttttg gccagatagg gtcccgaacc
     1861 aagataatct tgtgagattt tccatggtgt gctatagtga gcgggaagga aagttgcgga
     1921 ttgtgctgtt tttgtatgaa aagcaaggag gttcgagacc attttatgga agatcttcga
     1981 aagagagtca aggacgaacg ttcattcctt gaaaaagcag gaaaactgat acccggctac
     2041 tccgggtata aagaaaagtt gatccggcag gagggcgaca agcttctccg cgattggctg
     2101 gctggcaaga tgcagaccga gatggggtac ctcaaggacc tgattgatga aatgacccgc
     2161 tccatgaaaa tcaagctgct ggatcatgtc gatcgccccc tgaaaaagct ggagaaactg
     2221 cgtgacagga tcaaatttgc cgatcgtgga tacaccggct ggttcgctgc cattgatgtg
     2281 gacaaagact tgctcgatcg catgtacgaa ttcgacaatt cgttgattga tctcgtgacc
     2341 gaaatcgagc agttgatccg gtctgcccag gccgcggttt ccgatgatga caagctggtc
     2401 tcggcactca agtcgctgac cgatctcatt gcgcaggctg atgtcaatta caacgagcgg
     2461 gaaaatctca tactcaacaa ggcggagggg taaggcgcaa tggcactcac ttctggagta
     2521 cttgaatatt ttgatgaatc cgggaatgtc atggcacaga ggttgccgga agcaggttct
     2581 ggcgagttca agatgggcac atacgttgtt gtcagggaaa gccaggaggc tgtttttttc
     2641 cgtgacgggc aaatgctgga cacactcgga ccgggccggc atcagatcac gacggccaac
     2701 cttccgctga tcacaaagct tttttcgctg gtgacaactg ggggcgaatc cccattcaag
     2761 gccgagctct attttgtcag caagcagcca ttcctcaatg agggatgggg gacagaccag
     2821 cctattctct ttcgtgacaa agaattcggc atggtccgcc tgcgcgcgtt cggaatctgt
     2881 tctcaccgca ttattgacaa caacctgttt atcaacaagg ttgtgggtac ccgtggacgc
     2941 tatgcacgtg atgatgccaa agactttatc cgcaatatca ttgttggccg tttgaatgat
     3001 gccttgggcg aggtgatgga gacgatcctc gacctgccca agtattatga cgaagtcggc
     3061 gtggcgatga agtccaaggt tcgtgacgac ctcgagaagt atggaatcga gattgttgat
     3121 ttcatcatca aggcgatcac gccgcccgaa gaggtgcaga aggccatcga cgagcgcaca
     3181 tcgatgggtg ccatcgggaa tatgcagcag tacatgatgt tcaaagctgc aaaatccatg
     3241 caggatgctg ccaataatca aggcggtggt gccggaatgg gtatgggcat gggcgctggt
     3301 atcggcatgg gtatgatgat gcccggaatg atggcaggtg ctttcatgcc cggcatgcag
     3361 caaccgatgc agcccggtat gcaacaaccg atgcaacccg gcatgcagca gcccgtgcag
     3421 tcggcccagc cagaagcgcc agccgccggt ggaacattct gtaccaattg tggaacgcag
     3481 aatccggcag gcgccaaatt ctgtggcaat tgcggaaaca agatgggcgg ttaaccccgg
     3541 aaacccatgt tgtaaggata tcgccctgtt cgcctgcaga cggacagggt gctttctttt
     3601 ttcagacgct ttattcagtg gtatgagccg ccgataattg acgtgaatct ctctgtcgga
     3661 aatggtgcaa tcagcggcgg gataaaactt tcttggaaag gtcaccatgg tgcgtgtcag
     3721 tgctgcttct tcaggaatgc gtctggtctg tatgctggcg ctctttttct tctcgatgct
     3781 ggccctcctt ggcggtttca tcggcggtgt gttgctttcc tcgggaacct ggcacgaggg
     3841 agacatggcc gccctggttg aagagcgcgg catcggaagc cttttcacgc tcaatgatcc
     3901 agaccttgcc gtttttcatc ttgcggccga acgcctcttg gcgcagagcc cggtccttgc
     3961 cgtggatgtc ctgtcacggg atgccatact gtacagtaaa gcgatccaat ccaatgcagg
     4021 ggagagggca ttgccagcca actcggctct catccggcag atgggccgga tcatctgcca
     4081 cgatggccga ttgttgtacg cttgtcaacc ttcgttcgca agagagattc tggtcgtgct
     4141 tgaggtgaca caggaggacc ggccttggcg ggcgactctt tcccgggcca ttgccagagt
     4201 cggggaatgg ccggggtggg gtattcccgt catcattgcc ggttggtcgc tcctgtcatt
     4261 gactcttgca tggtgcattg tgtggatgtt gtattcaagg ccagtgtccc gggttgttgc
     4321 ggtgcttgaa gcctgtgaac aagtggagga tcttgcgact gcaaagcttt ttgggggaac
     4381 agtctccgat ccgcttgttg gctgtattga gcggctggtg cagcgtgcag gtgggggaaa
     4441 gaagacggga ttcgaaagtc ttctgccaag gcggcggggg tatgatttcc ccgggtttga
     4501 tgccgcgacg gtctatgccg ggacaagcga gaaactcgct gtgcatgtgg atatccggcc
     4561 tctcggtgac gggcgatatg gaatcatgtt gcttgcggtc agggctgaag atgagagcga
     4621 ggaagcgctg gttcggattg cgacggtctg cgacgcctgt ttcagcctgt atgtgaatcc
     4681 ggcgcaggtg gcgtcatctt tgaaccggta tatgcactcc atgtcgatcc cctttcccgt
     4741 ggatgccggt tttctggtct ggagtccatc cgaaaaaaaa ctcgagtttt gtctgaatgg
     4801 gtctgtcaac ctgttccggt atgatgttcg tgaggggacg ctgcgcggat atgatctcaa
     4861 gcagcctgtc ctggagggtg gactcccgga ggaattcgat gctggaatcg gttttgcccg
     4921 gcttgatatc ggagaggggg atcccgttgt gctggcctat gcgcccggcg tggaatggga
     4981 atcactggtg accgatgtcc tgaaaaaatc gacaacactc gcagaacggc aacaaaccct
     5041 caatgcgctt cttgggcaga gactcggacc gggagatgcg gcagccgtcc tcatgatgag
     5101 cctgtcagaa tgaagcgttt ccttgtcccg gtggtacttg tttactcgat ggttctctgt
     5161 ccggtgatgg gacttgctgc acaagagggc agcaaaccgg tttttcgtgt cccggttctc
     5221 tgctatcatg cggtcagtcc tgtggcggcg ggtcgctttg ccgtcacccc cgaagtcttt
     5281 tccatgcata tggaatacct gaagaaaaac gggcacaccg tgatttcggt ggcaggcctc
     5341 gtttcattct tgaaggcggt gcaaaaggga gagaaggtgt ctcttccgga aaagccggtt
     5401 gtgatcacct ttgacgacaa ttatgaatcg atccagaaac atgcgcttcc ggtgctcacg
     5461 aaatacaagt atccagcatg caatttcata tatcttgacg gaatctccgt agctggatgg
     5521 gagagataca aacggcttga aggatcgctg atggaactgc attctcatac agtcaatcat
     5581 gtcgatcttg caaaaaagaa acaggatgag gatgacaaat cctggagaaa gcgcattttt
     5641 cgcgagattg cggacagtcg gcggatcatc ggtgaaagac tggggcgctc tccggctttc
     5701 attgcctatc cctatggaac ctggaatgtc agtgtgatcc ggatgatgag gattgcaggc
     5761 tattctgctg gtttctccgc ctttggcgga tatgtccggg agacatcatc tctggatgtt
     5821 cttccacgat ttacaatatt cagggaatat gatttgcaga agtttgccga gattgtctcc
     5881 ggggcatggg tggggggaca aggtgagcct gattttatca gcgctcgtga ttacaacttc
     5941 agaatcgagt gagtgtcagg acgggccggt atgggcccgg ctttgagtgg ggaatgccgc
     6001 aaaccgacaa ggatgccgtg ttttctggct gcgcttgcac acgctctgct gtgcacaacg
     6061 gcccattccc gggctgaaga ctgttccacg aaaaagaact ggttcgtcgg aagctgattt
     6121 ctggtctgca gacacgggat ctcgtggtgc gcttccggtt gacgtgtttt tccatggagc
     6181 ctagaatggg agaatggcag ttttttcaag ggaggctgag gtgggacgaa aggaaattac
     6241 agggcggatt gtgaatcccg caagcggcga gatccgttcc gggcggattg tcttcgagga
     6301 cgggcgcatc ctggagatgc ttgcggatga ggatgttccc gagcgataca tcgttccggg
     6361 ttttgtcgat tcccatatcc acatcgagag ttccatgctc atgccctcac gctttgcggc
     6421 tgccgcattg cggcatggaa cccttgccgc agtctgcgat ccccacgaga tcgccaatgt
     6481 tctcggtctg gagggtgtac ggggttttct cgctgatgca gagaatgcgc ccttctcgtt
     6541 tgtgtttggt gccccgtctt gcgttccggc aacctctctt gaaacatcgg gtgccgccct
     6601 gacagcttgt gatatcaaaa cacttttttc ggaagacgga ttgaggttct tgggcgaggt
     6661 gatgaatgtc ccgggtgttc tggccgggga gaaagagctg gaagcaaagc tggatctggc
     6721 caaagaatac ggtttgccga tcgacggcca tgcacccggg cttctcggcg cagatctccg
     6781 ttcctacgtg gccgccggga tgacaacgga tcatgaatgc agtacggttg ccgaggccat
     6841 ggagaaggtc agacagggga tgtttgtcca gattcgggac ggatcggcgg ccaggaactt
     6901 tgatgatctg ctcccgctgt ttttccaggt tccggggcgg ttgatgttgg cgagtgatga
     6961 caagcatccg gatgacctgt tgggagggca tatcaatcgg atggtttcca gggccctcaa
     7021 ggcaggggtg ccgtttggtg cggttttgcg tgcggcatg
//
LOCUS       DAOSHE010000059         6891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9577_length_6891_cov_4.656671, whole genome shotgun sequence.
ACCESSION   DAOSHE010000059 DAOSHE010000000
VERSION     DAOSHE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9577_length_6891_cov_4.656671"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PLD82_02750"
     CDS             <1..1261
                     /locus_tag="PLD82_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64340.1"
                     /translation="QFNPATLEIAVPLEPGQYYMYKINASTKVVLWKSANAITVIGAV
                     VPVYNGETYAIYGDSGMSGKATAVKTTDGTSWTTIGTPGFSDGGNGTPALAVSKADGS
                     IWATHNWNYSNWYWKGGLYKFDGSSWSKKQDVLVGTHTEIGPMVMNNEGTAMFMGLGT
                     WSPWAGQMQKRTGTDFATASTFTMTGTGEGIANLLVDGDTLWLLTRKNYLYSDHSNLA
                     THGGLTLRKYNILNASFSEADFVLQGTAHFNMTSRPAFAPALAVYEGVAYVVFIDPEA
                     FGHYYHSSNGWNEQYTNPKLTVMKYDGSWSLVGSAQFSPVVYKTTRAGYDTGNNIGGE
                     IAPNTDTHDLYKGAICVGGGKVFVTATDSSDNGFIMQYNGSSWSTVTGNYGSGIRNPT
                     MFYDNGKLKIMYSKADGTGMVMYLHTL"
     gene            1665..5063
                     /locus_tag="PLD82_02755"
     CDS             1665..5063
                     /locus_tag="PLD82_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG64341.1"
                     /translation="MLEKMIRYFATRHMLANCMTIGVILAAVFAWEKTGKEETPNYEL
                     NFITIRTAYPGAAPEDVEQLVTKRLEEKLKSIDGIYRLQSTSTIGSSTIRIEFIPGYD
                     AMDEALNDIRNQTASVKLPAEVRDPPSVRQFKTTRRAVLDVAVYLTNHHTLDRTSRAA
                     LQKYAYDLSDQLGGLTQVSEVSMSGYYKNELQILLNTDRLREYNISTSEVIAVLKKHN
                     LRVPAGGLDDTLDTKVSVVAELDTVAKLSNLVIRASFDSPAIFLSDLATIRPGFEKNT
                     SIRKVNGHEAVTLNIRKTASSDILQTTDAVRAFIARYERDVLANTPARIQLLDDGSSG
                     VRNRLSLIVGNGILGFILILVFLFLFMDATSAFWVAMGIPFTFFVTMIAFPLMGYTVN
                     NITLAAIIIVMGMIVDDAIVVAENIARLRSAGIRQEDACILGTREMVLPVFGSIATTV
                     VAFVPLFFFSGRYSQMLVFIPPVIAVMLFASFFESIFVLPAHLGMHLPKPFMRFVSRL
                     GFFRRTLSVQQDPANGEKELKAHWFVKVEQWYARILEHLLHHRIIVTAFFIALLVTAG
                     LVFSSQFKFVLFPDEESNELNISGFAPQGSSKEQTEVLVRSIENLVNTERPANVVGYR
                     SVIAQSRRGGAADQSSFAIVIELKERENRTKSTEALMRQWREQVMKIPDLERVTVTAG
                     RFGSGSGSAIEVEVRENDDAIRKKAAEAVRDALDRLPGLRNAEVDEPIRNPEYRIGFN
                     RELVTRLGVNPSELATAMRTVLEGTIVDEINSGDEEVDIRVSVDGNYKQAIDFLLQNP
                     IQNSGDYLIPLSRLVSYERVLAPSSITRIHHKRTTMVYADIDDGKSDTQTRRPRKKPA
                     TNTIPKDHTADKLSVTDRNPGLPGEAAASTNRIRKTRVRPEPMTPIEVASWLEERIFP
                     KIQQQYPGVVFSFSGEIKDTRESSGDLGTATILVLFLIFVILALIFNSALRPAIVMMT
                     IPFGLVGIIFTFWIHGIEQFGLFAAIGALGLAGVVINDSIVMIDKLDRSIREDGWGKS
                     KFERIANISGTRLRAVMLTTLTTVAGLMPTAYGVAGYDAMLAQMMLALSWGLLFGTLI
                     TLILIPTIYTFLKDAAPAPVTGPLPEDTPATSPDQGSKPRKKSR"
     gene            5071..6591
                     /locus_tag="PLD82_02760"
     CDS             5071..6591
                     /locus_tag="PLD82_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG64342.1"
                     /translation="MKKLLLIGSTVCTLFSTPVTALLGAEIIQHKETNAQATAARLSL
                     QTFVERALENHPAMGKLRETWREQRNLAMKTLAIQELLLTASTGLTLTDPVYSGGAIS
                     ANQSTRWTMESGLTKTFPDLGGLTAGVSLGHGTTSTSDTDGSSTTVATPWVGLSLSIP
                     LIRNALGSADRASLKKMQLGLSIIDRSEEEAVELLIQSLAQEYYAWALASEKAAMYQG
                     FFERAGQYYNQVLRRANLGIADQSDIMLARQNWLGYQSSLLSANQTANELGLGILARI
                     GVRPDGNTNIRFLHTPDTSLPALKPDALVLSVSKTGPTNSISGFRIVQIARLSLEQAR
                     EEAGIARDAVLPDLTLSIKGTKTASDSSLDRAFSSMAGNTFFAGLVFSMPLQHSSDEY
                     SAEAAKNALARRTKEYESVVLDAGTALSRYRLAINTWRQLEKIAEDTSAAAAGRVAAV
                     YTRYSQGRATLNNLTDAQDAWARTKLQALETRYTLRRLELEFASLTDRLTRHVLPD"
BASE COUNT         1564 a         2197 c         1757 g         1373 t
ORIGIN      
        1 ccagttcaat ccggcaactc tcgagatagc tgtccccctc gagcccggcc agtactacat
       61 gtacaagatc aacgcctcga ccaaggtcgt tctttggaag agcgccaacg ccatcaccgt
      121 gatcggtgcg gtcgtccccg tctacaacgg cgaaacctat gccatctatg gcgactccgg
      181 catgagcgga aaagccaccg ccgtcaaaac gaccgatgga acgagctgga ccaccatcgg
      241 gactccgggc ttcagcgacg gcggaaatgg cacacccgca ctggcggtca gcaaggccga
      301 cggctcgatc tgggccacac acaactggaa ctattcgaac tggtactgga agggcggcct
      361 ctacaaattc gacggttcga gctggtccaa aaagcaggat gtactggttg ggacacacac
      421 cgaaatcggc ccgatggtga tgaataacga aggcaccgcc atgtttatgg gactggggac
      481 ctggtctccc tgggccggcc agatgcaaaa acgcaccgga accgatttcg ccaccgcatc
      541 gacctttacc atgacaggga ccggtgaggg gatcgccaac ctgcttgttg acggggatac
      601 cctctggctc ctgacccgga aaaactacct gtattcggat cactcaaatc ttgcaacgca
      661 cggcggactg accctgcgca aatacaacat cctcaacgcg agctttagcg aggccgactt
      721 tgtcctccag gggacggccc acttcaacat gacatcccgg ccggcctttg caccagccct
      781 tgcggtctat gaaggtgtcg cctacgtcgt gtttatcgat cccgaagcct ttggtcatta
      841 ctatcacagc tctaacggct ggaacgaaca gtacaccaat cccaagctga ccgtgatgaa
      901 gtacgatggc agttggtccc tcgtcggctc agcacagttc agtccggtcg tctataaaac
      961 aacgcgtgcg ggctatgata ccggaaacaa cattggtggg gagatagcac ccaacacgga
     1021 tacgcatgat ctgtataaag gagcgatctg cgtgggtggc ggcaaggtct ttgtgacggc
     1081 caccgacagc tcggacaacg gttttatcat gcagtacaac ggttcaagct ggtcgactgt
     1141 tacgggcaat tacggcagcg gcatccgcaa cccgaccatg ttctacgaca acggcaagct
     1201 caagatcatg tattcgaagg ccgacggcac gggcatggtg atgtacctgc atactctctg
     1261 agccgactac caaaacgaat ccgttttctg tccaaacaag gggcatccgg cacgggtgcc
     1321 ccgtgatgca tctccagccg gccaaaaaat ggcattacgt ttctgtcatg cgactccggg
     1381 agtatcgata ccatcatcac aatacaatga tgggcccccg gacgaagccc ccgtctcaag
     1441 aggtatacgc atgacggaaa acatgaaaat ttgatgctat tcccgagtcg caccacaaga
     1501 ttgcaaattc tgcggatgaa tcccggattc gcataacaat tttgtaatat ctttcttcac
     1561 tcggcggccg gtgacgacgg ttttttcgca gaaccctcca cagtttctac acatttccat
     1621 gctagattga atgggtgcaa gtccatgatt gagagagtcc acccatgctc gaaaagatga
     1681 tccgttactt tgccacccgg catatgttgg ccaactgcat gactatcggg gtcattctcg
     1741 ccgctgtctt tgcctgggaa aaaaccggca aggaagagac accaaactac gaactcaact
     1801 tcatcaccat caggacggcc taccccggtg cagcccccga agatgtcgag cagctcgtca
     1861 ccaaacgcct cgaagaaaag ctcaagagca tcgacggcat ctaccgcctc cagagtacgt
     1921 ccaccatcgg ctccagtacc atccggatcg aattcattcc cggatacgat gccatggacg
     1981 aagccctcaa cgatatccgc aaccagaccg catccgtcaa actccctgct gaagtccgtg
     2041 acccaccctc ggtccgccag ttcaaaacca cccgcagggc ggtcctcgat gtagccgtct
     2101 atctgacaaa ccatcacact cttgatcgaa cctcccgggc agccctccaa aagtatgcct
     2161 acgatctctc ggaccagttg ggcggcctga cccaggtcag cgaagtctcc atgtccgggt
     2221 attacaaaaa cgaactccag atcctcctca acacggaccg gctccgcgag tacaacatct
     2281 ccaccagcga agtgattgca gtattaaaaa aacacaatct ccgtgtcccg gccggcgggc
     2341 tggatgacac cctcgacacc aaggtcagtg ttgttgcaga actcgatacg gttgccaaac
     2401 tctccaatct cgtcatccgg gccagctttg attccccggc catttttctt tccgatcttg
     2461 caacaatccg gccgggattt gaaaaaaaca catccatccg caaggtcaac ggtcacgagg
     2521 cggtcaccct caatatccgg aaaacggcct cgagcgatat cctgcagacc accgatgccg
     2581 tgcgtgcctt tatcgcacgc tacgaacggg atgtcctcgc caacacaccg gcccggatcc
     2641 agcttctgga tgacgggtcc tccggggtgc gcaacagact ctccctgatc gtcggaaacg
     2701 gcatcctcgg cttcattctg atcctcgtgt ttctcttcct gttcatggac gccacctcgg
     2761 ccttctgggt ggccatggga attccgttca cgtttttcgt caccatgatc gcctttccct
     2821 tgatgggcta caccgtcaac aacatcaccc tggctgccat catcatcgtg atgggcatga
     2881 tcgttgatga cgcgattgtc gtcgccgaaa acatcgcccg cctgcgctcg gcaggtatca
     2941 gacaggagga cgcctgcatc ctcggcacca gggaaatggt ccttcctgtc tttggcagca
     3001 tcgccacgac cgttgtggcc tttgtccccc tgttcttttt ttcgggccgt tacagccaga
     3061 tgctggtctt tatccctccc gtgatcgccg tcatgctttt tgcctcgttt ttcgaatcca
     3121 tctttgtcct gccagcccat ctcgggatgc atctgccaaa gcctttcatg aggtttgttt
     3181 cgcgtcttgg cttttttcgg cgaacactct ccgtccagca ggatccggca aacggcgaga
     3241 aggagttgaa ggcgcactgg tttgtcaagg tcgaacaatg gtacgcccgg atcctcgaac
     3301 atctcctcca ccaccggatc atcgtcaccg cgtttttcat cgccctcctg gtcacggccg
     3361 gcctggtctt ttccagccag ttcaaattcg tcctctttcc cgacgaggaa tccaacgaac
     3421 tcaacatctc cggatttgcg ccccaaggct caagcaagga acaaactgag gttcttgtcc
     3481 ggagtatcga aaacctcgtc aacacggaac gcccggcaaa cgtcgtcggg tatcgctccg
     3541 tcatcgccca gagccgccgg ggtggcgctg ccgaccaatc ttccttcgca atcgtcatcg
     3601 aactcaagga gcgcgaaaac aggacaaaaa gcaccgaggc cctgatgcgc cagtggcggg
     3661 agcaagtcat gaaaattccc gatctcgaac gcgtcacagt cacggcaggc cgtttcggtt
     3721 ccgggtccgg gagcgccatc gaggtggagg tccgcgaaaa cgatgacgcg atccgcaaga
     3781 aggcggccga agccgtcagg gatgccctgg acagactccc cggtctgcgc aatgccgaag
     3841 tcgacgaacc gatccgaaat cccgaatacc ggatcggctt caacagggaa ctcgtcaccc
     3901 gccttggtgt caatccgtca gaactcgcaa ccgcgatgcg cacggtcctt gaggggacta
     3961 ttgtggatga aatcaattcc ggtgacgagg aagtggatat cagagtctcc gtcgacggca
     4021 actacaaaca ggccatcgac ttcctgttac aaaacccgat ccagaacagc ggagactacc
     4081 tgatcccgct ctcgcggctt gtctcgtatg aacgtgtcct cgccccgtcc tcgatcaccc
     4141 ggatccacca caagcgcacc accatggtct acgccgacat cgacgacggc aaatccgata
     4201 cccagacccg ccgaccacgg aaaaagccgg caaccaacac catccccaaa gaccatactg
     4261 ccgacaagtt gtccgtgaca gaccgcaacc cgggacttcc gggagaagca gccgcgtcga
     4321 ccaaccgcat ccggaaaaca cgcgtccgac ccgaaccaat gaccccgatc gaggttgcca
     4381 gctggctcga agagcgtatt tttccaaaaa tccagcagca gtatcctggc gtcgtcttca
     4441 gcttcagcgg cgagatcaag gacacccgtg agtcaagcgg ggatctggga accgccacaa
     4501 tactcgttct cttcctgatc ttcgtgattc tcgcgctgat cttcaattca gccctccggc
     4561 cggccatcgt gatgatgacc atccccttcg gactggtcgg catcatcttc accttctgga
     4621 ttcacggaat cgaacaattc ggactctttg ccgccatcgg ggccctcggt ctggccggcg
     4681 tcgtcatcaa cgattccatc gtcatgatcg acaaactgga ccgttccatc cgcgaagacg
     4741 gttgggggaa gtcgaagttc gagcggattg ccaatatctc gggcacgcgc ctgagggccg
     4801 tcatgctcac caccctgacc acggtggcgg gtctgatgcc gaccgcctat ggtgtcgccg
     4861 gatacgatgc catgctggcc cagatgatgc tcgccctctc ctggggactc ctcttcggga
     4921 cactgatcac cctcatcctg atcccgacca tctacacctt cctcaaggat gccgcaccgg
     4981 cgcccgttac cgggccgctt cctgaggata cacccgccac gtctccggac caaggcagca
     5041 aaccgcgcaa gaaatcgagg taagaccgtc atgaagaaac tcctcctgat cggcagcacc
     5101 gtctgcactc tgttctcgac gcccgtcacg gcgctccttg gtgccgagat tattcagcac
     5161 aaagaaacca atgcacaggc cacggctgca aggctctcgc tgcaaacctt tgtcgaacgt
     5221 gcgcttgaaa accaccccgc gatgggaaaa ctccgggaga cctggcggga gcagcggaat
     5281 cttgccatga agacgctcgc catccaggaa ctcctcctga cggcatcaac cggtttgact
     5341 ctgacggatc ctgtctacag cggcggggcg atatcggcca accaatccac acgctggacc
     5401 atggagagcg gcctgacaaa aacctttccc gatttgggtg gtctcaccgc cggagtcagt
     5461 cttggccacg gaacgacctc caccagcgat acggatggaa gcagtacaac cgtcgccacg
     5521 ccttgggtcg ggctctccct gagcatcccc ctgatcagaa acgccctcgg ttcagccgac
     5581 cgggccagtc tcaaaaaaat gcagctcggg ctttccatca ttgacagaag cgaggaggaa
     5641 gccgtcgaac tcctcatcca gagcctcgcc caggagtact acgcatgggc tcttgccagc
     5701 gaaaaagcgg ccatgtacca gggttttttt gaacgagccg gccagtatta caaccaggtc
     5761 ctgcggcgtg ccaacctggg catcgccgac caatcggaca tcatgctggc ccgtcaaaac
     5821 tggcttggct accagtccag cctgctcagc gccaaccaga cagccaatga actcgggctc
     5881 ggtatcctgg cgcggatcgg ggttcggccg gacggaaata cgaatatccg gtttctccat
     5941 accccggaca ccagcctccc ggccctcaag ccggatgccc tcgtactcag tgtttcaaaa
     6001 acgggcccca caaacagcat atccggcttc cggattgtcc agattgccag gctctccctc
     6061 gaacaggccc gcgaagaagc cggaatcgcc cgggacgcgg tccttcccga tctgaccctc
     6121 tccatcaagg gaaccaagac cgcttcggat tccagcctcg atcgtgcctt ttcttccatg
     6181 gccggaaata ccttctttgc ggggcttgtg ttctcgatgc ctctccagca ttcttccgat
     6241 gagtattccg ccgaagccgc caaaaacgcg cttgcgcgcc ggacaaagga gtatgaaagt
     6301 gttgtcctgg acgccggaac agccctcagt cgctaccggc tggcgatcaa cacctggcgg
     6361 caactcgaga agatcgccga agacacaagc gccgccgcgg ccggccgggt ggccgctgtc
     6421 tacactcgct attcacaggg ccgggcaacc ctcaacaatc tgaccgatgc ccaggacgcc
     6481 tgggccagaa caaagctcca ggccctcgag acccggtaca ccctgcggcg gcttgaactg
     6541 gaattcgcct ccctgaccga ccggctcacc cgtcacgttc tccccgattg agggatgatt
     6601 cctgtttgat atcacaatac tccagaattg tgctggctat ccaggattca cattgcagag
     6661 atgcgtctcc cgacgctttt ccgtcgccgg gagaatttga ctttctccgc cggcggggtg
     6721 tattctggat actgccggct ggtccatagc ggcgggaaaa cgtccctggt ggagaccgca
     6781 tgcaccagca gattaccatc ctccttgtcg aagatgagcc gatcgtcaga cttgccacaa
     6841 aacgcaagct ggagcggttt ggatacgcag ttgagacagc cgaagatggc c
//
LOCUS       DAOSHE010000060         6891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9578_length_6891_cov_3.725424, whole genome shotgun sequence.
ACCESSION   DAOSHE010000060 DAOSHE010000000
VERSION     DAOSHE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_6891_cov_3.725424"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1266)
                     /locus_tag="PLD82_02765"
     CDS             complement(79..1266)
                     /locus_tag="PLD82_02765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64343.1"
                     /translation="MRWTKNRLLKNGDQIHLGPGARLEWRSGNGERHILRGPASALIT
                     GESLALESGRYWRGSRPEPGLELRWLPSPPARGRITRLQAALRGAEGQVSLQTLPERS
                     TSVLHFDGIRGGQRLHSAMIALARTDWRRELRLEVRIATPQGTLTEILAFPVSAATET
                     PLDTRGIFGRRAGIALLWHWRAAPSFFPYQGQVLRPEQCVAWKETQETGKQFPAWTQG
                     GTSQGIWLPPDKQALLADQAGIQRENAIHWQATSWDTPEILWTGPFIIPAWHRYSSPF
                     SESRTFVGLGTGVHLGIDIASFYGTAVAAPNSGIVRFAGKTTLCGNNIIIDHGRRVFT
                     KIMHLDTLFVASGQRVERGQPVGMTGTSGLSTGPHIHWEMLVGGVRVDPEEWVALREG
                     GLQ"
     gene            1671..3155
                     /locus_tag="PLD82_02770"
     CDS             1671..3155
                     /locus_tag="PLD82_02770"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOG64344.1"
                     /translation="MASRIERFGDLWYLLLEGDARTRGRDHAGLVRGITGTETADYYA
                     RLLPTIIRQQRPRWLPRFAVRSAAALISGFFGTMQAGIDAGSLAVEMQAFLIGYRRGG
                     AKRLIRFPDVLHWLAGMAGGQCSAVFFEGGVHSRLARNFDFYGPRVWGQHRAIKVSIP
                     PSGQPWLWAGPLGIPVGGFGVNASGIAVMPFTNFTRDLSLRGLPLFTLMQTILEESHS
                     LDDALAYLERARRHGGISLLVADMQRGETAAIAVGARRLLVDRPRDGVLVRTNHHHEL
                     DRPPQPGMSMWQRHSRSRWQRLDRLARDLPPGGGPDPLALRQLLADRTQPDGRRSLLS
                     DVPGALNVATSIILDGMKLFCADAGFPACDATRWHGFSLDECFSGHLPDVETHELPDL
                     LSQEERAAIALTVEGWSRYFDESQPSAARDVWSQARAHAPREPALLRMLAMAAHDEGD
                     RTACREYLESLLELDPGDEVARLALKRPGQVYLECMTGQLAPRR"
     gene            3378..4769
                     /locus_tag="PLD82_02775"
     CDS             3378..4769
                     /locus_tag="PLD82_02775"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOG64345.1"
                     /translation="MEHRKSPWINFLFFMADACMTMLAALLSIFLRQIIPWGGEAILP
                     AEHYVPFLLLFIPVLLLTMLFAGQYRYNRGVSILLEFMSIVRLVFLAVVAMLALSFFY
                     REYQFSRLHIVYFFTLAVVLIFTGRVVIRLWLRRVRSSGRNLMHIVVLGEGRRLESFV
                     REMRSVAGYGYSVDGVFSLTTGRTGSGLPYSKKALQVWLEQHRMDEAYILLGSGDRHE
                     KRMREALELFEREGVRTRLVPDLDSSLATGARMALIGNIPVLTIAEYPLDFWYNRAIK
                     RAFDILVSLTGLVFSSPLFLLIALAVRLSSPGPVFFRQERLGLDRRKFTIIKFRTMYR
                     QEKQTSDTVWTVKNDPRVTHLGRLLRVSSLDELPQLVNVLKGEMTLVGPRPERQHFVN
                     EFKKRMPLYMERHRVKAGLTGWAQVSGYRGDSSIERRLQADLYYIRNWSFTFDIRIML
                     LTLVRVLSGRNAH"
     gene            4766..6166
                     /locus_tag="PLD82_02780"
     CDS             4766..6166
                     /locus_tag="PLD82_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG64346.1"
                     /translation="MTESAQRTLIVGAGEAGRMIAQGIRADGRVAGRYHLVAFVDDDP
                     AKKTVAGIPVRHSIDALREIVLEDAIDVIFIAVPSAGGALLGRIIDRVGDLPIRLRIL
                     PGLREIIDGDASWRALRDVRPEDLLGREEIQFERERLAAFYEDARVMVTGGGGSIGSE
                     ICRQLLALPVARVVAFGHGENSLYHLQQELGGDPRFVCQIGDVRDAEKIERTIAAWSI
                     SDIFHAAAHKHVPLMEAFPDEAVKNNVYGSFVVARAAGRARIRRFLMVSTDKAVRPSS
                     VMGATKRVAERIVLGMNGDGSGTRYAVTRFGNVLGSRGSVLPLFVEQIRRGGPVTVTH
                     PEVTRYFMSIREAARLVVKAATLDCGEIFVLDMGQPVNIHELARRLVMLSGRHPDEIP
                     IVITGLRPGEKLHEELWTEHEDCAASPFEKLLVARDSGGRMETAEVQRFIESMDPPLS
                     SGDREAVRSILFTAAR"
     gene            6481..6708
                     /locus_tag="PLD82_02785"
     CDS             6481..6708
                     /locus_tag="PLD82_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG64347.1"
                     /translation="MEYRLPLRDAYIRLSDALKLGGLVETGGLAKILVQEGAVRVDGV
                     VVTERGRKLRPGMIVECRGHRLVIGTREEGL"
     gene            6705..>6891
                     /locus_tag="PLD82_02790"
     CDS             6705..>6891
                     /locus_tag="PLD82_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64348.1"
                     /translation="MKKVIAPEATYRILSNGPLLMVVTLGQSGDGLRPEVSTVAWSTP
                     IDTNPPRLAVIIDSTHRT"
BASE COUNT         1176 a         2068 c         2219 g         1428 t
ORIGIN      
        1 accccaccct tcctcgcggc gggagaaaat ctggctgcag tttgagtcca atcctgatgc
       61 ctgcggtgta tccgctgact actgcaaccc gccttcgcgc agggcgaccc attcctcggg
      121 gtcgacgcgc accccgccga ccagcatctc ccagtggatg tgcggacctg tcgagagccc
      181 ggaggtccct gtcatgccca cgggttgtcc gcgttcgacc cgttgcccgc ttgccacaaa
      241 cagcgtgtcg aggtgcatga tcttggtaaa gacgcgccgc ccgtgatcga tgatgatgtt
      301 gttgccacag agggtggtct tgcccgcgaa gcggacgatt cccgaattgg gtgcggcgac
      361 cgcggttcca taaaaagaag caatatcgat gccaagatgc accccggtac ccagaccgac
      421 aaaggtccgc gactccgaaa acggcgagga gtagcggtgc caggccggga tgatgaaggg
      481 ccccgtccag agaatttcgg gagtgtccca ggatgtggcc tgccagtgaa tcgcgttttc
      541 ccgttggatc cccgcctggt ctgccaggag ggcctgcttg tcgggaggca gccagatacc
      601 ctgtgatgtc ccaccctgcg tccatgcggg gaattgtttg ccggtctctt gtgtctcttt
      661 ccaggctacg cactgttccg gccgcagaac ctgcccctgg tacggaaaaa acgatggagc
      721 ggcacgccag tgccagagca aagcgatgcc tgccctgcgc ccgaagattc cccgggtatc
      781 gagcggggtc tcggtagcgg ccgagaccgg gaatgcgagg atctccgtca atgtcccttg
      841 gggcgtagcg atccgcacct cgaggcggag ttcccgcctc cagtccgtcc gcgccagggc
      901 gatcattgcg gaatgcaacc gctgtccgcc ccggattccg tcaaaatgaa gaacggatgt
      961 actccgctcc gggagtgtct ggagggagac ctgcccctct gctccgcgga gcgcggcctg
     1021 tagccgggtg atccgtcccc gggcgggcgg tgatggaagc cagcgcagtt cgagtccggg
     1081 ttcaggtctg ctcccgcgcc agtaccgccc gctctccagc gcaaggcttt cgccggtgat
     1141 gagagcactg gccggtccac ggaggatgtg ccgttctccg tttccgctgc gccattcgag
     1201 tctggcgccc ggaccgaggt ggatctggtc gccatttttg aggagccggt ttttggtcca
     1261 acgcaccggc caggcaccgg agtccggcgc ggctgcgaag ggatccgcga caggcaaaga
     1321 tggcgggctg agccggatat caccttcggc aaccactggc tgcagatcgg catttgccgg
     1381 gggcgccagc acgagcagga gcgcagagac cagccagcct cgccatgcac gccggaaaca
     1441 cgatctttcg gagagatggt cggcggaacc ctgtttgcag taccggaaag aatggagccg
     1501 tcccgttgaa ggatggaagc aggaatccgc gaaaccgtga tctgtttccc ggagtcgtgt
     1561 gacggaatca tgaatatatg tacaatacgc atccgcactt gagatcgggc gggcgcctgt
     1621 gctatcatct cgcatggtta gagtatagcc agaaggacgg ggtgggggaa atggcttcgc
     1681 ggattgaacg ctttggggat ctctggtatc tcctcctcga gggcgatgcc cggaccaggg
     1741 gccgggacca cgccgggctg gtgcgcggga tcacggggac ggagacggcc gactactatg
     1801 cccgcctctt gccgaccatc atccgccagc agcgtccgcg ctggttgccg cgctttgccg
     1861 tccggtcggc tgctgccctg atcagcggtt tttttggaac gatgcaggcc ggaatcgatg
     1921 cggggagtct cgcggtcgag atgcaggcct tcctgatcgg gtaccggagg ggtggagcaa
     1981 aacgtctgat ccgttttccc gatgtgctgc actggctggc cggaatggcg ggagggcagt
     2041 gttcggccgt gtttttcgag gggggtgtgc actcccggtt ggcacgcaat ttcgactttt
     2101 acgggcctcg tgtctgggga cagcatcgcg ccatcaaggt gtccattccg ccatccggcc
     2161 agccctggct ctgggccggc cctctcggga ttcccgtcgg cgggttcggg gtgaatgcct
     2221 cggggatcgc cgtgatgccg tttaccaatt ttacccggga cctttccctg cgtgggcttc
     2281 ccctctttac cctgatgcag acgattcttg aggaatcgca ctcgctggat gatgcgctgg
     2341 catatcttga acgtgcccgg cggcacgggg gaatctccct tctggtggcc gacatgcagc
     2401 gtggggagac ggcagccatt gcggtcggag cacgccgcct tctcgtcgac cgtccccggg
     2461 atggcgtgct ggtgcggacc aaccaccatc atgaacttga cagaccacct cagcccggga
     2521 tgtcgatgtg gcagcgccat agccgctcgc gctggcagcg gttggacaga ctggcccggg
     2581 acctcccgcc gggaggcggg cccgatcccc tggccctgcg gcaactcctg gcggaccgga
     2641 cccagcccga tggacggcgc tcccttctgt ccgatgttcc tggcgccctc aacgtggcga
     2701 cctcgatcat tctggacggg atgaagctct tctgtgccga tgccgggttc ccggcctgcg
     2761 atgcgacccg ctggcacgga ttttcactgg acgaatgttt ttccgggcat ctccccgatg
     2821 tggaaacgca cgaactcccc gacctgctct cacaggaaga gcgggctgcg attgcgctga
     2881 cggtggaggg ctggtcccga tactttgacg agagccaacc aagcgccgcc cgcgatgtct
     2941 ggagccaggc ccgggcgcac gcgccccggg aacccgccct tctgcgcatg ctggccatgg
     3001 cggcccatga cgagggggac cggacggcct gccgggaata ccttgaaagc ctgcttgaac
     3061 ttgatcccgg tgacgaggtg gcccgcctgg ctttaaagcg gccggggcaa gtctacctcg
     3121 aatgcatgac gggccagctt gccccccgca ggtgagtccg gattggagat ccgggaacgc
     3181 gtgtgcatcc gtcgtgacag tccgggagag gggaaaggca ggggacgccg taccccataa
     3241 aaaccggaaa tgctcgacgg cccatcctgc ggaccttgag atctgtctgg gccgaagact
     3301 cgccgtccat gcgtggtggt tgcgggctgg actgccggga cgtgctatat ttggaacggg
     3361 gaaggcaggt acaagcgatg gagcaccgca agagtccgtg gatcaatttc ttgttcttta
     3421 tggccgatgc ctgcatgacg atgctggccg ccctgctctc gatttttttg cggcagatca
     3481 ttccctgggg cggggaagcc atcctgcctg cggaacatta cgtccctttt ttgctcctct
     3541 ttatcccggt cctcctcctg acgatgctct ttgccggaca gtatcgctac aaccgggggg
     3601 tttccatcct cctcgagttc atgtccatcg tgcgccttgt gtttctggcc gttgttgcca
     3661 tgctagccct ctcgtttttt tatcgcgagt accagttttc ccgtctgcac atcgtctact
     3721 ttttcaccct ggcggtcgtg ctgattttta ccgggcgggt cgtgatccgg ctctggctgc
     3781 gccgggtccg ttcgagcggg cgcaatctga tgcatattgt cgtgttgggg gagggacgcc
     3841 ggctggagag ttttgtccgc gagatgcggt ccgttgccgg ctacggctac agtgtagacg
     3901 gcgttttttc cctgacaaca ggccggaccg gttcgggact gccctactca aaaaaggcgc
     3961 ttcaggtctg gctcgagcag caccggatgg acgaggccta catcctcttg gggagcgggg
     4021 accggcatga aaagcgcatg cgcgaggcgc tcgaactctt cgagcgtgaa ggggtcagga
     4081 cccggctcgt ccccgatctg gattcctcac tggccacggg ggcgaggatg gccctgatcg
     4141 ggaacatccc cgtcctgacg attgccgagt atccgctcga tttctggtac aaccgggcca
     4201 tcaaacgcgc cttcgacatc ctggtctccc tgacgggact tgtcttctcc tctccgctct
     4261 ttctcttgat tgccctcgcc gtgcgcctct cttctcccgg accggtcttt ttccgtcagg
     4321 aacggctggg tctggatcgc aggaaattca caatcataaa atttcgcacc atgtaccgac
     4381 aggaaaaaca gacctcggat acggtctgga cggtcaagaa cgatccccgg gtcacgcacc
     4441 ttggccgcct gttacgggtc tcctcgctgg atgagctgcc ccagttggtc aatgtcctca
     4501 agggcgagat gaccctggtc gggccccgtc ccgagcggca gcattttgtc aacgaattta
     4561 aaaagcgcat gccgctctac atggaacgac accgggtcaa ggccgggctg accggttggg
     4621 cccaggtcag tggctatcgg ggagacagtt ccatcgagcg ccggctccag gcggatctgt
     4681 attatattcg aaactggtcg tttacttttg acatccgcat catgctcttg acgctggtcc
     4741 gggtgctctc gggcaggaac gcacattgac ggaatcggca caacgtaccc tgatcgtcgg
     4801 agccggtgag gccggacgga tgattgcgca gggaatccgc gcggacgggc gggtcgccgg
     4861 ccgctatcac cttgtcgctt ttgtggatga cgacccggcc aagaagactg ttgccgggat
     4921 tccggtgcgt cattcgatcg atgccctgcg cgagatcgtg ctggaagatg ccatcgacgt
     4981 gatctttatc gccgtcccct cggccggcgg cgcgcttttg ggccggatca tcgaccgtgt
     5041 cggggatctt cccatccgcc tgcgcatcct tcccggcttg cgcgagatca ttgacgggga
     5101 cgcatcctgg cgggccctgc gtgatgtgcg gcccgaggac ctcctggggc gcgaggagat
     5161 ccagttcgaa cgtgagcggt tggcggcctt ctatgaggat gcccgggtca tggtgacggg
     5221 cgggggcggc tcgatcggat ccgaaatctg ccgccagctt ctggccctcc ccgtggcgcg
     5281 ggtcgtggcc tttgggcacg gggagaacag cctgtatcat ttgcagcagg aattgggcgg
     5341 ggacccgcgc tttgtctgcc agatcgggga tgtccgtgac gccgaaaaga tcgagcgcac
     5401 gatcgcggcc tggtcgatca gcgacatctt ccacgcagcg gcccacaagc atgttcccct
     5461 gatggaagcc tttcccgacg aggcggtcaa gaacaatgtc tacgggagtt ttgtggtggc
     5521 ccgggccgca ggccgcgccc ggatccggcg cttcttgatg gtctcgaccg acaaggctgt
     5581 ccggccgagt tcggtgatgg gagccacaaa acgcgtggcg gaacggatcg tactggggat
     5641 gaacggggac ggctccggga cgcgctatgc cgtgacccgc ttcgggaatg tcctcgggag
     5701 ccggggctcg gtccttccgc tctttgtcga gcagatccgc cgcggcggtc cggtgaccgt
     5761 gacccatccc gaggtgacgc gctacttcat gtcgatccgc gaggcggccc ggctggtcgt
     5821 caaggccgcg acgctcgatt gcggcgagat ctttgtcctc gacatgggac agccggtcaa
     5881 tatccatgag ctggcccgac gactggtcat gctctccggc cgccatcccg acgagatccc
     5941 gatcgtgatt accggactcc ggccgggcga aaaactgcac gaggagctct ggaccgagca
     6001 cgaggattgt gcggcctctc cgtttgaaaa gcttctcgtc gcccgggaca gtggcgggcg
     6061 gatggagacg gccgaggtgc agcgcttcat cgagtcgatg gacccgcccc tgtcgagcgg
     6121 tgaccgggag gccgtgcgca gcatcctctt tacggcggct cgttgatccg gttttttttg
     6181 gtgaacactc tcaggcgact gacgagcagt ctggagcggg agtgggtgcc ggcctatgtg
     6241 gtccttgttg cttaaaattt ggtggaaaaa tgtaatccgg gattccccac tgacgacttt
     6301 tgtatagcct caaaaaccgg aaattgcgaa aattcagtgg ttttttgcag aaacgcggag
     6361 gaagtacagt gtagtgtttg cttaaaatgt aagtaatttg gtatatccct tcgggatctt
     6421 cgccgggatt gtgtgctgtg cacttgcacc tttcagttcc ttgtgactat gttatccggt
     6481 atggaatacc gtctccctct tcgtgatgcc tatatccggc tctctgatgc actcaaattg
     6541 ggtggcctgg tcgaaacggg cggcctcgcc aagatcctcg tccaggaagg ggcggtccgt
     6601 gtggacggcg ttgttgtgac cgagcgcggc cgcaaactcc ggcccgggat gatcgtcgag
     6661 tgccggggac atcggctcgt gatcggtacc cgggaggagg gtctatgaaa aaggtgattg
     6721 ctccagaggc aacgtaccgg atcctctcca atgggcctct cctcatggtt gtgactctgg
     6781 gccagtcggg ggatggactc cgccccgagg tttcgacggt ggcctggtcc acaccgattg
     6841 acaccaatcc accccgcctg gccgtgatca tcgacagtac gcaccgcaca t
//
LOCUS       DAOSHE010000061         6869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9620_length_6869_cov_4.072791, whole genome shotgun sequence.
ACCESSION   DAOSHE010000061 DAOSHE010000000
VERSION     DAOSHE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_6869_cov_4.072791"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLD82_02795"
     CDS             complement(<1..694)
                     /locus_tag="PLD82_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG64349.1"
                     /translation="MPARLILALVLLLSLAVHLPAASTVFTLPNGLETIVFPRPDVPL
                     VSIQFSIRHGYIYANDPLDEGIAHFYEHMLFKANSLTKTQQEFMRRLNEIGAAGWNGT
                     TGSEAVTYYLTVPATEAERALELWSAVLIDTVFDPPEIRREISVVSNEIEGNRRDPGR
                     AFWRLVNAALYGQDQMYRTGHYETAHMGRFDRALLQREQARWYTPDNCMLSLSCAIDE
                     REALQLVSKHFSR"
     gene            829..2199
                     /locus_tag="PLD82_02800"
     CDS             829..2199
                     /locus_tag="PLD82_02800"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOG64350.1"
                     /translation="MAIVLKDCFYVGTLDKGLELAGADIVIEGNRIIAVGKDAGKSVA
                     SPERVIDCSRHVVVPGFVNTHHHFYQTLTRNLKAVQDAKLFDWLIYLYEIWKHLDRDA
                     IYWSTMLAAGELLKTGCTTATDHHYVYPHGFAGDIMETQFEAAGRLGMRFSPTRGSMS
                     RSKKDGGLPPDSVVQTEDQIMADSERVLAKYHDAGEFSMRKIVLAPCSPFSVTKDLMR
                     ESARLARRYGANLHTHLAETQDENDFCVEFYGKRPLALMEECEFIGKDVFYAHGIHFN
                     DEELALLTKTDTKVAHCPSSNMRLGSGIARVKEMLERGMTVGIAVDGSASNDASDFLG
                     EMRNAMLLQRVRYGSDALTARQVFEMAAQHGARVLNYSKIGTIAPGYAADLACFDVHR
                     MDFAGSLSDPLAALLFCGYSHTTDYTIVNGKVVVDKGHLVGVDEDELFKKGHEISTTM
                     VAKAGA"
     gene            complement(2643..2969)
                     /locus_tag="PLD82_02805"
     CDS             complement(2643..2969)
                     /locus_tag="PLD82_02805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG64351.1"
                     /translation="MNTTDDILHDEHGQRFYFEHDGQTAELTYRVLENGTLDYNHTFV
                     PEALRGGGLAGDLVLAACKYARSTGKKILPSCSFVDMWLKRHKEFSGLVDTTLDCNPA
                     CRIDKR"
     gene            complement(3011..3376)
                     /locus_tag="PLD82_02810"
     CDS             complement(3011..3376)
                     /locus_tag="PLD82_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG64352.1"
                     /translation="MDDAELFRNWLENNPPEPSAKSSGEPQHGPRSRPSRTRLDLHGH
                     TAAASEDLVRLFLRQARFAGIRQVEIITGRGLNSGKPGGILKHHIATFLDSGKAGVIR
                     GWRRGNPHEGGDGTMIVDL"
     gene            complement(3771..5699)
                     /locus_tag="PLD82_02815"
     CDS             complement(3771..5699)
                     /locus_tag="PLD82_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64353.1"
                     /translation="MKKLSLFCACTALLILLSGTLLDALPGQQMARLRVTLAAESTVE
                     SAQIFVNGQAKGTVYRGRASEFILPFSQRATVRLVHGASVVERQVNLVPGRWVEISIP
                     VVTQTKGTIRVQLDAGPDNAQVFVDNAFKGTVNRGQSLVLPNIDAGTRQVRVQNGSAQ
                     QTRAVTVTPGQLASVNFSLQVVPVTGEVSVRLDPTSAVATASVLIDGTKRATILKGRA
                     LSFTLAAGNHTIRLEQGNLFKDLTVSLSAGQKMDANFILTEPEGSLRFVLERGRTSRV
                     STARVDINGARKATVVPGRTYLWNLPAGQHTVRVQSGAVEATETVTIIAGQTTPLTMT
                     LRPPMGRLSVTHGSDSSVYTATLFINGASRTTINKGATMAFDLEPGTYTVQLKNGALE
                     SESVPITLASGQNLSASLTVKKPMGKVNLTYSGESAHAWATIYIDGNQSDRIRRGQTK
                     SYELETGFHTFEVRAGNEKAEERIMLSQRRERNLTLKIEPTMGRLAVTLGGDSNVPNA
                     QVTVNGQSRGTVNRGQTLTIENLAPGTVAVVLSGNGITEQGQAQITRGQTASISLSLK
                     QQMGSLLVTLLPGSRAAFARVVIDGVYRFTVNRGESKTLELPAGVHTVILEGGRAPQT
                     IRQVVETGKVSRIVMNLQ"
     gene            complement(5817..6230)
                     /locus_tag="PLD82_02820"
     CDS             complement(5817..6230)
                     /locus_tag="PLD82_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64354.1"
                     /translation="MRPDCAHPVIAILDLILGLTFLVFFLFIVPARYPLITIFFVLLG
                     LTTLASAWGLWIGKQWGRLLARITSAVAITFGTGFTLLCLGAASYLSGVYGGMGKASS
                     SIFFVMMGLALILLVLLPSIQLFHLRNSVRPAGDD"
     gene            6324..>6869
                     /locus_tag="PLD82_02825"
     CDS             6324..>6869
                     /locus_tag="PLD82_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64355.1"
                     /translation="MNIRIRRFLVGLAMLFTAVFVLFSWNAGRPPETPAVSRQQALTV
                     MQAIRETLVRPETRWPLPDLSGLGIRSVFLHLDRVRWFRGSGTDGVIALSNLVQVLRL
                     YASTNTNGHCEIFLSFAGPVKDLVGEGPLFWSLTVQQGLDGIALRHEDGRQAWLLPEE
                     GGNEIWAGGEPFGFIPFRFGLS"
BASE COUNT         1391 a         1887 c         2145 g         1446 t
ORIGIN      
        1 agcgggaaaa atgttttgac accagttgca gggcttcccg ttcatcgatg gcacacgaga
       61 gggagagcat gcagttgtcg ggtgtgtacc agcgggcctg ttcgcgctgg aggagggcgc
      121 ggtcaaagcg tcccatgtgt gcggtttcat aatgccctgt ccggtacatc tgatcctggc
      181 cgtagagggc cgcattgacg agccgccaga aggcccggcc aggatcacgc cggtttccct
      241 cgatttcgtt gctgacgacc gatatttcgc gccggatctc gggcggatca aagacggtat
      301 cgatcaggac agcggaccag agttcgaggg cccgttcggc ctcggtcgcg ggcaccgtca
      361 ggtaataggt gacggcctcg ctgccggtcg ttccattcca ccccgccgca ccgatttcgt
      421 tgaggcggcg catgaactcc tgctgggtct tggtcaggct gttggccttg aagagcatgt
      481 gctcgtaaaa atgggcgatc ccttcgtcca agggatcgtt ggcgtagatg tatccatgac
      541 ggatcgaaaa ctggatcgaa accaggggca catccggacg gggaaagacg atcgtctcca
      601 gcccgttggg aagggtaaag acggtggatg cggccgggag atggacagcc agcgagagga
      661 gaaggacgag cgcgaggatg agacgggccg gcatggggac ctccagagtt gacacagcgg
      721 attcggttaa aatgtagcat ttttttggcc ccggccacct tccctcttga ttgccggagt
      781 gcggcgttgt atcctctaca aacagaatgt tgaccacctg gaggaaccat ggccattgta
      841 ttgaaagact gtttttacgt cggaacgctt gacaagggac tcgaactggc aggcgccgat
      901 atcgtgatag aaggaaaccg gatcatagcc gtcgggaagg atgccggcaa gagcgtcgct
      961 tctcccgagc gggtgatcga ctgctcacgg catgtcgtcg ttccgggttt tgtcaatacc
     1021 catcaccatt tttatcagac actgacgcgc aacctcaagg ccgtgcagga cgccaagctc
     1081 tttgactggc tgatctatct gtacgagatc tggaagcatc ttgaccggga tgccatctac
     1141 tggtcaacca tgctggcggc aggcgagctc ctcaagacgg gttgtacgac ggccaccgac
     1201 caccactacg tctatccgca cgggttcgcc ggggacatca tggagaccca gttcgaggcg
     1261 gccggacgcc tggggatgcg cttctcgccg acccgtggct cgatgtcccg cagcaaaaaa
     1321 gacgggggat tgccgccgga ttcggtggtc cagaccgagg accagatcat ggcggacagc
     1381 gagcgggttc tggccaagta tcatgatgcg ggcgaattct ccatgcgcaa gatcgttttg
     1441 gcaccttgca gtccgttttc ggtgaccaag gatttgatgc gcgaatctgc ccgtctggcc
     1501 cgccggtacg gggccaatct ccatacccat cttgccgaga cccaggacga aaacgatttc
     1561 tgcgtcgagt tctacgggaa gcgtccgctg gcgttgatgg aagaatgcga gttcatcggg
     1621 aaggatgtgt tttatgcaca cggcatccac ttcaacgacg aggagctcgc gctcctgacg
     1681 aagacggata ccaaggtggc ccattgtcca tcgtcgaaca tgcggctcgg atcggggatc
     1741 gcccgggtca aggaaatgct cgagcggggc atgacggtcg gaatcgcggt ggacggctcg
     1801 gcctccaatg acgcgtcgga ttttttgggc gagatgcgca acgccatgct cctccagcgc
     1861 gtgcgctacg gctccgatgc cctgacagcc cgccaggtct tcgagatggc cgctcagcac
     1921 ggggcccggg tcctcaatta ttccaaaatc gggacgattg cgcccgggta tgcggcggac
     1981 ttggcctgct ttgacgtgca ccggatggat tttgccgggt cgctcagtga tcccctggct
     2041 gcgctcctct tctgcggata cagccacacg accgactata ccatcgtcaa cggcaaggtt
     2101 gtcgtggaca aggggcatct ggtcggcgtg gacgaggacg agctcttcaa aaaaggccac
     2161 gaaatatcga ctacgatggt cgcgaaggcc ggagcgtgag cgaaggacca aagcctgatc
     2221 gtccgtggtc gtgaaacgcc tgatacacca ttccggggcg tctgcggttt gcagtcggcc
     2281 cggtttttgt atgctgaaca acctcagtcc ggcaaccggc gagcacaagc gcggcaggtc
     2341 cgccgaagcg ttggtctcgg gcccgtggtg gtgtgagttc tcagacgaaa ggaacgggca
     2401 ggacgaagag cctgccttca tggtatggca gacatcacct gaaaaacggc tggctcttgc
     2461 agaataaggc tgtggggatt gcagcactgg gtgtcccggg ccatggacaa gagcctcagg
     2521 gctcttcatg gtctatgcac ccgagattgc acgccaatgt cgaagaagcc acgcctcttg
     2581 tacgaaactc cctgccctga ttgcttcatg tcggagattg aattctgaaa accctcggca
     2641 gatcagcgtt tgtcgatcct gcaggcaggg ttgcaatcaa gtgtcgtgtc cacaagaccg
     2701 gagaattcct tgtggcgttt gagccacatg tcgacgaagc tgcaagaggg gaggattttt
     2761 ttaccggtgg agcgggcata tttacaggcg gccagcacca gatcgcccgc gagcccccca
     2821 ccgcgcaacg cttccgggac gaaggtgtga ttatagtcca gtgttccgtt ctcgaggacg
     2881 cgataggtca gttcggcggt ctgcccgtcg tgttcaaagt aaaaacgctg tccatgttcg
     2941 tcatgcagga tgtcatcggt cgtgttcatc attcctccag gatcgtcttt ttttaaatat
     3001 atataaatat ctataaatcg acaatcatgg tcccgtcacc gccctcgtgg ggattgccgc
     3061 ggcgccagcc ccggatgaca ccggcctttc cggaatccag aaacgtcgcg atgtggtgtt
     3121 tgaggattcc gccgggtttt ccggaattca gtccccgtcc cgtgatgatc tcgacctggc
     3181 ggatgcccgc gaagcgggcc tggcgcaaaa agagccggac caggtcttcg gaagcggccg
     3241 ctgtgtgccc gtgaagatcc agccgggtcc gtgaggggcg ggaccgtggg ccgtgctgcg
     3301 gttctccgga ggatttggcg gacggttcgg gaggattgtt ttcgagccag ttgcggaaaa
     3361 gttcagcatc atccatgggc gcctcccgtc caggttcttg tgtcaataat agcatacatc
     3421 tgcccggtgc aatcccggat agtcagattg tctgaatgga agcccgcctg agcagtcagc
     3481 gcttgatcgg gaatgcgtac gggccggttt ttgaccctga atgagccact gattgctgct
     3541 ccattcacga tctccggaca ggtattggca agctccgggc ctgttcgacg ggatccatgc
     3601 agcaatccgg gccatcaggg atatcgggtg tgaagcgcgg agtatctggc ctcagacccc
     3661 gaaaatgtcc cttgttccaa aacggaccgt tgattttgtg gacggtgact ttgaaaaaag
     3721 gggctggcca aatcggcagc cccttgtcca tgacggattt gtcaaacaga ttactggagg
     3781 ttcatcacga tccgcgagac cttcccggtt tccacgacct gacggatcgt ttgcggggcg
     3841 cggcctcctt cgaggatgac cgtatgcacc ccggccggaa gttcgagggt tttggattca
     3901 ccacgattga cggtgaagcg atagacaccg tcgatgacaa ccctggcgaa tgccgctcga
     3961 gaaccgggta gaagggtgac caagagactg cccatctgct gtttgaggga gaggctgatc
     4021 gaagcggtct gtccccgtgt gatctgggcc tggccctgtt ccgtgattcc gtttccgctc
     4081 aggacaacag cgaccgttcc gggggcaagg ttctctatgg tcagtgtctg tccgcggttg
     4141 accgtccccc ggctttgtcc gttgaccgtc acctgggcgt ttggaacatt tgaatcccca
     4201 ccgagggtca ccgccaggcg tcccatggtc ggctcgatct tgagggtaag gttgcgctcg
     4261 cgcctttgcg agagcatgat ccgctcttcg gctttctcgt ttcctgcccg gacttcgaaa
     4321 gtgtggaaac cggtttcgag ttcatagctt ttggtctgtc cacggcggat ccggtcggac
     4381 tggttcccgt cgatatagat cgtcgcccag gcatgggcgg attcacccga ataggtcaga
     4441 ttgacctttc ccatgggctt tttcaccgtc agtgaggccg acaggttttg gcccgaggcg
     4501 agcgtgatgg gcacagattc cgattcaaga gcgccatttt tcagttgtac cgtataggtg
     4561 cccggttcaa ggtcaaaggc catggttgca cccttgttga tcgtggtccg ggaagcgcca
     4621 ttgatgaaca gggtggccgt gtagaccgag gagtccgatc cgtgtgtgac cgagaggcgc
     4681 cccatgggtg ggcggagtgt catcgtcagc ggagtcgtct ggccggcaat gattgtgacg
     4741 gtctctgtag cctcaaccgc accactctgg acacggacgg tgtgttgccc cgcaggcaga
     4801 ttccagaggt aggtccgtcc ggggacaacc gtcgccttgc gggcaccatt gatatcgaca
     4861 cgggccgtgc tgacccggct ggtccgtcca cgctcaagaa cgaagcggag ggatccttcc
     4921 ggttcggtca agatgaaatt tgcgtccatc ttttggcctg cgctgaggga aaccgtcaga
     4981 tccttgaaga gattgccctg ctccagacgg atcgtgtggt taccggcagc aagggtgaag
     5041 ctcagggctc ttcctttgag gatcgtcgcc cgcttggtcc cgtcaatcag gacagaggcc
     5101 gttgccactg cggacgtggg atcgagtctg acactgactt ccccggtcac cgggacaacc
     5161 tggagcgaga agttgacact ggcgagctgg cccggggtga ccgtcacggc acgggtttgc
     5221 tgggcgctcc cgttttggac acggacctga cgggttccgg catcgatatt tggcaacacc
     5281 agggattgtc cacggttgac ggttcccttg aaagcattgt cgacaaagac ctgtgcgttg
     5341 tccgggccgg catcaagctg aaccctgatc gtcccctttg tttgggtaac gaccgggatc
     5401 gagatctcca cccagcggcc gggaacaaga ttgacctgtc gttcaacgac gcttgccccg
     5461 tgcacgagcc ggacggttgc ccgctgcgag aaggggagaa tgaattcaga agcccgtccg
     5521 cgatagactg tgcctttggc ttggccgttg acaaagattt gtgccgattc gacggtcgat
     5581 tcagcggcca gtgtcacccg cagtcgggcc atctgttgcc cgggaagggc atcgagcaag
     5641 gttcccgaga gcaggatcag gagtgcggta caagcacaaa acagggaaag ctttttcata
     5701 cactgtttcc tccaaaaaag agtgagtgtg ctgcaatcat gcagaaacgg acgggagacc
     5761 cgtccatcac acgaaagaca tgaaaaatca catagctgat ctgacacccg caggagtcaa
     5821 tcgtcacccg cgggcctgac gctgttcctc aaatgaaaga gctggatcga aggcaggaga
     5881 acgaggagga tcagggcgag acccatcatg acaaagaata tcgaagaaga ggcctttccc
     5941 atgccaccgt acacacccga gagataggac gccgcaccca ggcagagaag ggtgaaccct
     6001 gtcccgaagg tgatggcaac ggcacttgtg atcctggcga ggagccgtcc ccactgcttc
     6061 ccgatccaga gaccccaagc cgaggcgagg gtcgtcagcc cgagcaggac gaaaaagatg
     6121 gtgatcaggg ggtagcgggc cggaacgata aacaggaaga acaccagaaa ggtcagaccg
     6181 aggatgaggt cgagaatggc gatgacgggg tgtgcgcagt ctggtctcat gagaacagta
     6241 tggttccgat ctggacgctt gtcaagtccc atcaagactg atatgctggg gatgcgtaca
     6301 aacggtcttt catgggggag agattgaaca tccggatccg tcggtttctc gttggcctcg
     6361 ccatgctgtt cacggctgtc tttgtccttt tctcgtggaa tgctggccgt ccgccagaga
     6421 ctcccgccgt gagccggcag caggcgctca cggtcatgca agccatccgc gagacacttg
     6481 tcaggccgga gacccgatgg ccccttccgg atctttccgg tctcgggatc cggagtgtct
     6541 ttctccacct ggaccgggtg cgttggtttc gcggatcagg cacggatggg gtgattgccc
     6601 tgtccaacct tgtgcaagtg ctccggctct atgccagtac caataccaac ggtcattgcg
     6661 agatctttct ctcgtttgcc ggaccggtca aggatctggt cggggagggt ccgctctttt
     6721 ggagtctgac ggtccagcag ggtctggacg ggatcgcgct tcgccatgag gacgggcggc
     6781 aggcctggct cctcccggaa gagggtggaa acgagatctg ggccggggga gaaccgttcg
     6841 ggttcattcc cttccgcttc ggtctctcc
//
LOCUS       DAOSHE010000062         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9770_length_6786_cov_3.980984, whole genome shotgun sequence.
ACCESSION   DAOSHE010000062 DAOSHE010000000
VERSION     DAOSHE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9770_length_6786_cov_3.980984"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLD82_02830"
     CDS             complement(<1..542)
                     /locus_tag="PLD82_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG64356.1"
                     /translation="MALTAIPQPYANGFQDPDELCLSYTRFGDGSGSLALTRADFARL
                     SGRAASLLAKHECHHGTRILHCFGDNDWMDPVFRMGAAMLGAIPVAINWQADSLEQIV
                     YKFEKPRASAIVFNESFDTELRANLISRLGTAVCIDTREILEETELPQPEGYRTVPGD
                     TRLIVFTSGTTGNPKGVMLT"
     gene            complement(558..2570)
                     /locus_tag="PLD82_02835"
     CDS             complement(558..2570)
                     /locus_tag="PLD82_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64357.1"
                     /translation="MRLRYALLGILAGLSLNIHGGELLTGTGESMDLRGIDLESAPVP
                     LTGFWQCRPLAPEPGPWRPARLPALFSELDLPGDGLVEFSLVLQLDPKMEFVGFLIPV
                     LHSAHTLLLDREIVLNRGAPGPSADRTVHHWEPGSVIHPVKNGRMEITLRVANWGYYK
                     GGIPAPLLVGSTAVITSRETARSRYEAFLTGSLIMIGLYQLGAFILRRRELAPLFFSL
                     FCLLIALRILVTGEHRIHQILSGMSWDLEYRIFMLTQTLGVLSFLGFITAVYGNAVLK
                     RTIRFTRRFFETASLFCLLAPTALICRSGIVLDAATIGVLGLTLAIIVRALQQKRDGS
                     LIMLSGFLVLAVTIVHDIIVENTVEHDTLLILPLGLFLFVLSQAWLLAFRFSRAFTIV
                     EDLTKNLESKVLDRTAELEKERNRLHQHGERLKKELEMARTIQMQMIPTASPSPAIAF
                     FYQPMEEVGGDFFDFIPLEDGSLGVLVSDVSGHGVPAALITSMIKSHFQQAGCKAGTP
                     GSVLHDLNGQISQRTGGNFVTAFYGILDPGRRKMTWASAGHNQPLIISPRDGVRTLDS
                     GIRRLPLAVLHNSELPSPDGYWMDNTVQLPSNGKILLYTDGLVEAVKIDGSSDGDFET
                     LALSDTLKHLAASPAHECVQGLVRALQDFRGAETFDDDVCIICIDT"
     gene            2876..4549
                     /locus_tag="PLD82_02840"
     CDS             2876..4549
                     /locus_tag="PLD82_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG64358.1"
                     /translation="MRRVLVAAVVLILGAGRLFSLTTVSTNAGEVPPNSLVRKIHIRV
                     KEVFPEDKNFFFDVANAFHARTAEATIRNELLYDEGEPYNQDLVDESERNLRKLNICV
                     VERTRAVPVPGEPGQVDVFVEVRDIWSLYFNIKLESSGGVTRYNFKWGERNFLGRNLS
                     LELELDRDTFFNRWRQHFDEPRLFGSRWRFFERVGFYYDHDGNKVGEGIELYLIRPLY
                     ARSEKWGWQTSFQYDDEYVIKNEGDRIKEVEVAPGVFLPRKYYDKSYVLENALIRSFG
                     YTNKFNIGLYVRNEQNRYRGYDGLPAEYADAFRSQVMNEDSTRHKLGLLLDANNHRFV
                     RIRNLNNYGRIEDLALGTSIRTTLSASRTFWGSDENGWYSTITLSNTAMIGDNHISMV
                     MASFSSDFVAGLGQRDMVTTLRYVHHIRNLPLGTLSLRAETSIGERLENDSLLSLGSD
                     KGLRGYVSNRFEGDRRVVFNVEYRFDPLPFIENPWIAFAAFYDLGACWFNHERRLADV
                     RFYPAIGLGLRLSIPSLNPAMMRFDFGFNLGNGPSTIGSIFSFGYNHAF"
     gene            4738..5322
                     /locus_tag="PLD82_02845"
     CDS             4738..5322
                     /locus_tag="PLD82_02845"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOG64359.1"
                     /translation="MAKLYFRYSAMGAGKSLDLLKVAYNYDERGMKALIFNSELDTRF
                     GRECVASRAGIHKNAIPYGVSSDFRKLYRETREALGAAVHCLLVDEAQFLTKTQVEDI
                     VHIVNDEKIPVICYGLRTDFMGQLFEGSTWLLAWADAIEELKTVCWCGRKATMNARIV
                     DGRIVRQGEQVQIGGNESYIALCREHYLSGELER"
     gene            complement(5295..6566)
                     /locus_tag="PLD82_02850"
     CDS             complement(5295..6566)
                     /locus_tag="PLD82_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64360.1"
                     /translation="MTHTTARRLILESALREKLQPRDATALHDHCAACPACQRFLASI
                     QANGRAILPVPEPRPSFESDLFDSIMHRSAQPTRVSVPPPRRLPGFRSALAVAASIPA
                     ILVSFLLTRGVLSPDALPPAHRTVVSAEQPADPATLTIARETSLPASEITLSIEQGTR
                     QIPIPPVQSRKQEPSRESAKRDIADQDAALGANLPRPSLARREDATTAEAEILQSRTG
                     ITADSLAKAKTDKLQPGAKLSLNMEESKTAETGKGTSERAAAPDRKATAEKKSALRAT
                     TDDATPTLFRYQPTARLHVEVPDQDAARRIQEHRQRLEQLMTGLMPEGTSISIHPVVK
                     TGRAALKREVPLPESAPDPWKTIVLILWLVFEAGVRILNIIAGSARRSPGLGRFDTAL
                     GAILGTLWLPVILVRLVSTPPADQRSSSPER"
     gene            complement(6563..>6786)
                     /locus_tag="PLD82_02855"
     CDS             complement(6563..>6786)
                     /locus_tag="PLD82_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG64361.1"
                     /translation="ESATLDNETVSEVRAAVNALDETYRPVFLLYYFEEFSLAEIART
                     IGIPVGTVKSRLHNGGKKLAELLARRAAP"
BASE COUNT         1393 a         1812 c         2148 g         1433 t
ORIGIN      
        1 caagtcagca tgaccccctt ggggttcccc gttgttccgg aggtaaagac gatcaggcgg
       61 gtgtccccgg gtactgtccg gtacccctcg ggctgtggca gttccgtttc ttcgagaatc
      121 tcccgggtat cgatgcagac cgccgtcccg agccgggaga tcaggttggc gcggagttcg
      181 gtgtcgaaac tctcgttgaa gacgatggcc gaggcccgtg gcttttcgaa cttgtagaca
      241 atctgttcca gcgagtcggc ttgccagttg atggcgacgg ggatcgcccc gagcatggcg
      301 gcacccatgc ggaagaccgg atccatccag tcattgtccc cgaagcagtg aaggatccgt
      361 gtgccatggt ggcactcatg ctttgccagc aggctggccg cgcggccgga gagacgggca
      421 aaatcggcac gggtcagggc gagactcccg ctcccgtcac cgaaacgggt gtacgaaagg
      481 cagagttcat caggatcttg aaaaccgttt gcatagggtt gcgggattgc tgtcagggcc
      541 acgggattct cctcgggtca ggtatcgata cagatgatgc acacatcgtc gtcaaacgtt
      601 tcggccccgc ggaaatcttg cagggcgcga acaaggcctt gaacgcattc atgcgcggga
      661 ctcgctgcca ggtgcttgag ggtatcggag agtgcgaggg tttcgaaatc tccgtccgaa
      721 ctcccgtcga tcttgacggc ttcaacaagc ccgtccgtat agagcaggat tttcccgttt
      781 gaaggaagct ggactgtgtt gtccatccag tacccgtccg gggagggaag ctcgctgttg
      841 tgcaagaccg caaggggcag gcgcctgatc ccgctgtcga gcgtccggac tccgtcccgt
      901 ggactgatga tcaggggttg attgtgtccg gcggaggccc aggtcatttt ccttcgcccg
      961 ggatccagga ttccgtaaaa ggcggtcaca aaatttccgc cggtgcgctg gctgatctgt
     1021 ccgttcaggt catggagaac gctgccgggt gttccggcct tgcagccagc ctgctggaaa
     1081 tggcttttga tcatggaggt aatcagggca gccggcacgc catggccgga gacatcggag
     1141 acgaggacac ccagggatcc atcctcgagg ggaatgaaat cgaaaaagtc tccgccgacc
     1201 tcttccatgg gctgatagaa gaacgcgatc gcaggcgagg ggctggctgt cggaatcatc
     1261 tgcatctgga tcgtcctggc catctccaat tcttttttca gccgctcgcc gtgctggtgc
     1321 aggcggttcc gctccttttc aagctctgcc gtgcgatcca ggaccttgct ctcgagattt
     1381 tttgtcagat cctcgacgat ggtgaacgca cgggaaaacc ggaaggcgag gagccatgcc
     1441 tgcgagagga caaagagaaa gagtccgagc gggaggatca ggagggtgtc atgctcgacg
     1501 gtgttttcaa cgatgatgtc gtggacgatc gtcacggcga gaaccaggaa tcccgagagc
     1561 atgatcagac tgccgtcgcg tttttgttgg agggccctga caataatggc aagggtcagt
     1621 cccaggactc cgatggtggc ggcatccagg acaatcccag agcggcagat cagtgctgtc
     1681 ggtgccagaa ggcagaacaa cgaggcggtt tcgaagaacc gtcttgtgaa ccggattgtc
     1741 cgcttgagga cagcgtttcc gtagactgcg gtgatgaatc cgagaaagga gagcacaccc
     1801 agcgtctggg tcaacatgaa gatccggtat tcgagatccc aggacatccc ggaaaggatt
     1861 tggtggatcc tgtgttcccc cgtgacaaga atcctgagag cgatcaggag gcagaagagg
     1921 gagaaaaaca ggggggccag ttcgcggcga cggaggatga aggcacccag ttgatagagt
     1981 ccgatcatga tcagactccc cgtcaggaag gcttcgtacc ggctgcgtgc cgtctctctg
     2041 gatgtgatga ctgcggttga gcccacgagg agtggtgcgg ggatcccgcc cttgtagtac
     2101 ccccagttgg cgacacggag ggtgatctcc atccggccgt tcttgacggg atggatgaca
     2161 gaacccggct cccaatgatg gacagtcctg tcggctgacg gccctggcgc tccccgattc
     2221 agcacgattt cccgatccag aagcagtgtg tgtgccgaat ggaggacggg aatcaggaaa
     2281 ccgacaaatt ccatttttgg gtccagttgc aagacaaggg aaaattcgac caatccgtca
     2341 ccgggcaggt cgagttcgga aaagagggcc gggagtcggg ccgggcgcca ggggccgggc
     2401 tcgggtgcta gcggacggca ctgccaaaag cccgtcaggg ggaccggcgc ggattcgagg
     2461 tcgatacccc tgagatccat ggattccccg gtcccggtca ggagttcccc gccatggatg
     2521 ttcaggctga gtccggccag gatgccaagc agagcgtatc gcagtctcat ccatcctcca
     2581 tgaccagggt tggcgcgaca gtggccagta tgcgtgacgg gggacaacct gtcaatcctg
     2641 aagatggcgg atgtccggtg aaaacggtaa atatcatgaa taagtaatgg gaatcctgtc
     2701 ctcttgcggg tactgcaccc gggagggaag gccacatcat gaaagaaaaa caagtctgga
     2761 gcagggacga ttttcaggcc accccccgtg aaaaccccgc tttttctttg gaggaatcca
     2821 tgctatactc tccttccagc gaaactcggg agaggacagg gtctgtcagg tgaagatgcg
     2881 gagagtactg gttgcggctg ttgttttgat ccttggcgcg ggcagactgt tttcactcac
     2941 gaccgtatcc accaatgctg gcgaagttcc tccgaacagc cttgtccgga aaatccacat
     3001 ccgggtcaaa gaggtgtttc ccgaagacaa gaattttttt ttcgatgtgg ccaatgcctt
     3061 ccatgcacgg acagccgagg cgacgatccg caacgaactc ctctatgacg agggagagcc
     3121 gtacaaccag gatcttgtgg acgaaagcga gaggaatctg cgcaagctca acatctgcgt
     3181 tgtggagcgg acgcgggcgg tcccggttcc gggagaaccc ggccaggtcg atgttttcgt
     3241 cgaggtcagg gatatctgga gcctgtattt caatatcaaa ctggaatcct ccggtggtgt
     3301 gacgcgctac aacttcaaat ggggtgaacg gaactttttg ggacggaacc tctccctcga
     3361 actggaactg gatcgggata catttttcaa ccgctggcgc cagcatttcg acgagcccag
     3421 gctattcggg agccgctgga ggttcttcga gcgggtcggg ttctattacg atcacgacgg
     3481 aaacaaggtc ggggaaggaa tcgaactcta cctgatccgg ccactttatg cgcgcagtga
     3541 aaaatggggc tggcagacgt ccttccagta cgatgatgaa tatgtcatca agaacgaagg
     3601 cgaccggatc aaggaggtcg aggttgcgcc cggcgtcttt ctgccccgca agtactacga
     3661 caagagctat gtgctggaaa acgccctgat ccgttccttc gggtacacca acaaattcaa
     3721 catcggtctg tatgtacgga atgaacaaaa ccgctaccgt ggctatgacg ggcttccggc
     3781 cgagtatgcg gatgccttcc gcagccaggt gatgaatgag gattcgaccc gccacaagct
     3841 cggtcttctc ctggatgcca acaaccaccg cttcgtccgt atccgcaatc tcaacaatta
     3901 cggccggatc gaggacctgg ccctcgggac ttccatccgc accacgcttt ccgcgtccag
     3961 gacgttttgg ggctctgacg agaacggctg gtattccacg atcacccttt ccaatacggc
     4021 catgatcggc gataatcaca tttcgatggt tatggcgagt ttttcgtccg attttgtggc
     4081 cggcctgggc caacgtgaca tggtgacaac cctgcgctat gtgcatcaca tccgcaatct
     4141 ccctcttggg accctctcct tgcgggctga gacatctatc ggagaacggt tggagaatga
     4201 ttcccttctc agcctcggtt cggacaaggg gctgcggggg tatgtttcaa acaggtttga
     4261 aggcgaccgc agggtcgtat tcaatgtcga gtaccgcttc gatccgcttc cgtttatcga
     4321 gaatccctgg atcgcttttg ctgcgtttta tgacctgggt gcctgctggt tcaatcacga
     4381 acgtcgcctg gccgatgtcc gcttctaccc tgcgattggt ctgggcctgc gcctctcgat
     4441 ccccagcctc aatccggcca tgatgcggtt tgattttggt ttcaacctcg gcaacgggcc
     4501 aagtacaatc ggcagcattt tttcattcgg atataatcat gcattctgac agcatacacg
     4561 gatgcggttt tggatgcggg ggatgtgctg atttttatgg gttccgcaga aaagcaaaaa
     4621 gcagtgcagg cttgtctgga tggcaggaaa gtatgctgac cagcgttgta tggtatacag
     4681 ggacgatcgt gacgagggtc ttgtctctcc cgcggcgccg gaaagggatg gtgtgtaatg
     4741 gccaaactct attttcgata ttctgcaatg ggagcgggaa agtcactgga tctcctcaag
     4801 gttgcctata actacgatga acggggcatg aaggcactga tcttcaattc ggagctggac
     4861 acccgcttcg ggcgggagtg tgttgcctcc cgtgccggta tccacaaaaa tgccattccc
     4921 tatggggtct caagcgattt tcggaaactc tatcgggaga cacgggaggc actgggggcc
     4981 gctgtccact gtctcctcgt ggatgaagcg caattcctca ccaagaccca ggttgaagac
     5041 attgtccaca tcgtgaatga cgagaagatt ccggtcatct gctacgggct gcggaccgac
     5101 tttatgggcc agcttttcga ggggagtacc tggctcctgg catgggcgga tgccatcgag
     5161 gaattgaaga cggtttgctg gtgcgggcgc aaagccacga tgaacgcacg gatcgtggac
     5221 ggccggattg tccgccaggg tgagcaggta cagatcggcg ggaacgagag ctatattgct
     5281 ctctgtcggg aacattatct ctcgggtgaa ctggagcgct gatccgcggg cggggtggag
     5341 acgagacgga caagaatcac cgggagccag agcgttccca aaatggcccc gagggccgtg
     5401 tcaaaccggc caagtccggg cgagcgacgg gccgagcccg cgatgatgtt caggatgcgg
     5461 actccggcct cgaagaccaa ccagagaatg aggacgatgg ttttccaggg atccggcgct
     5521 gactccggga gcggaacctc gcgtttgaga gccgcccgac cggtcttgac gacgggatgg
     5581 atcgagatgc ttgtgccttc cggcatgaga ccggtcatca gttgctcgag gcgctggcgg
     5641 tgttcctgga tccttcgggc cgcatcctga tcgggaacct cgacatgcag gcgggctgtg
     5701 ggctggtacc tgaaaagggt cggtgtcgca tcgtccgtag tcgcgcgcag ggccgatttt
     5761 ttttcggctg ttgccttcct gtccggcgca gcggctcttt cggaggtgcc ctttcctgtc
     5821 tcggctgttt tcgactcttc catgttcaga gagagttttg cgccgggctg aagtttgtcg
     5881 gtctttgctt tcgcgagact gtcagcggtg attcctgtcc gggactggag aatctcggct
     5941 tcagcagtcg ttgcgtcctc cctccgcgcg agagaaggtc gtggcaggtt tgcgccaagg
     6001 gccgcgtcct gatcggcgat atctcttttg gctgattccc gcgagggttc ctgtttgcgg
     6061 gactgcactg gcggaatggg gatctgccgg gttccctgct cgatcgagag ggtaatctcc
     6121 gaagccggca ggctcgtctc ccgggcgatg gtcagggtcg ccgggtcggc cggttgttca
     6181 gcagagacca ccgtccggtg ggccggcggc aaggcgtccg gggagaggac tcccctcgtc
     6241 agcaaaaagg agacgagaat cgccggaatg gatgcagcca ccgccagggc cgaccggaat
     6301 cccggtaagc gacggggcgg aggaacggac acccgggttg gttgcgcact ccggtgcatg
     6361 atcgagtcga agagatcgga ttcgaaggaa ggacggggtt cgggaaccgg aaggatggcc
     6421 cgcccgttgg cctgtatgct ggccaagaag cgctggcagg ccgggcaggc tgcacaatgg
     6481 tcatgcaggg cagtcgcatc cctgggctga agtttttccc ggagtgcgct ctcgaggatc
     6541 aggcgacggg ccgtggtgtg ggtcatggcg cagccctcct tgcgagcagt tcagccagtt
     6601 tctttccgcc attgtgcaga cgggatttga cggttccgac cggaatcccg atggtcctgg
     6661 cgatctcggc cagggagaac tcctcaaaat agtagagaag aaaaaccgga cgataggttt
     6721 cgtcaagggc gttgacggcg gcacggacct cggacaccgt ctcgttgtcg agcgtggcgc
     6781 tttcgg
//
LOCUS       DAOSHE010000063         6748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9843_length_6748_cov_4.539818, whole genome shotgun sequence.
ACCESSION   DAOSHE010000063 DAOSHE010000000
VERSION     DAOSHE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9843_length_6748_cov_4.539818"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLD82_02860"
     CDS             complement(<1..945)
                     /locus_tag="PLD82_02860"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOG64362.1"
                     /translation="MRMKNCFLAWYAVFLVIPSGLVQAQADALQLSESLAVEKNFRSG
                     YYFYNRREYTAAVSFFDQAISVNPHDHRSRIWLGQSFYMAGFQKNAMTEWQVALNLGA
                     GGSLLRNKLKNVYAIETREDAYDEASPWIFLKGFDGYRDKKSLFVRPSGLAIDENRNL
                     YIAGFASGKVSILDPNGNLLNVLMAGLSKPYDLAFAPDGSFYVSDFGNDSVVRYSRSG
                     QKLAIIGRLGYRASELAGPEGIHVDKTGALYVADSGNNRIQKFSSQGKYLMSFGRKGR
                     GEGEFFRPSDVLVLEDGRILVSDSGNGRLQLFDESGNFI"
     gene            complement(979..1275)
                     /locus_tag="PLD82_02865"
     CDS             complement(979..1275)
                     /locus_tag="PLD82_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64363.1"
                     /translation="MDVRLDVTHQQPALQRPYAGQSRKDRAGRVEAVESERVEEERPE
                     TAQKATVAPPQVISREELQQFLLLLGTTRGSEELLSAMVHDASKMRGMLLGGKT"
     gene            complement(1333..2049)
                     /locus_tag="PLD82_02870"
     CDS             complement(1333..2049)
                     /locus_tag="PLD82_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64364.1"
                     /translation="MIRQKDIDGYLAVARKDLDENRVKSSIKELVKIEKLDPENLEAQ
                     YLLGIALAKTGEYGEAVPHLERVVHSEYGYLHVQQACMLLGVIAVRREVWEDAEQYFL
                     RVLRFNFNNDAACSALGHVYFVRGEIEKSLQMLQQALKLNPDNHNARNSLAYVYCEGG
                     GDLDRALMEAMKASKAEPRNYAFLDTMGWIYYKKGKETLARETLKRALELAPENEEIK
                     EHLRVVLGMGQDQVVEQVYR"
     gene            complement(2123..2896)
                     /gene="surE"
                     /locus_tag="PLD82_02875"
     CDS             complement(2123..2896)
                     /gene="surE"
                     /locus_tag="PLD82_02875"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG64365.1"
                     /translation="MKHILLVNDDGINAPGLAELERVFKTQPDFAVSVIAPDRQRSAN
                     SHFVNLWSPLRLSEHGPARYAIDGTPADCVKLGHFFLETPVDLVVSGINSGQNMGVDV
                     YYSGTVAAAREAIINGLQGMAVSLSSKDEAADYRFAAKVALEQARVLLERPLPAGTLL
                     NVNVPDLPETGIRGVKVTRLGKRVYRDGLDRRMTPFGQTYFWLSCDGMSFTPCEDGDL
                     DAVHAGYVSLTPVLLDATDYRSLESLAGRGVGREADPVV"
     gene            2987..3739
                     /gene="map"
                     /locus_tag="PLD82_02880"
     CDS             2987..3739
                     /gene="map"
                     /locus_tag="PLD82_02880"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG64366.1"
                     /translation="MPPHIKNSEQIAAIRTLAGELSELLAWSLGQVRAGITTQAIDMH
                     IETRMREMGVRGPCKGYHGFPTVSCISVNSQVTHGIPDGTILREGDLVDIDIVIERHG
                     YFADMSRTITVGEASPQAQKLIRTTEECLAYGISAARPGGTLGDIGAAIQNHAEKNGY
                     SVVRDYCGHFIGIAMHEDPLVPNFGKKGRGTKLQPGMILCIEPMINEGSWQVITRGWD
                     ARTADGKLSSRCEHMVLITPTGHEVLTRFLPY"
     gene            3790..5736
                     /locus_tag="PLD82_02885"
     CDS             3790..5736
                     /locus_tag="PLD82_02885"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOG64367.1"
                     /translation="MLAQRSLSSRMFLVFSVLVAIILTGLVIAGTFLTSNTVTTSSQR
                     ELVQIAEGIRNTMHAGYTFSIERIAGVLDDFESRSAKQFFVNKQASDMLDVTSNSGAT
                     EGNRTLPRLLYGQNPVLHSHELSDRHARLSGGVFAIYQRAGDDLVQVSTSLRHPDGKR
                     ATGEILPGKGIPAGTVLSGKEFSGRLLVTGRWYISILRPLMDPDGSIIGAVLCGMPAS
                     NLSSIRSIVHGLQIGNTGMVGYAMVIDSDGIAVIHPSHESRSLLDNTDIHGYKYIRDW
                     LSRKNGTARFETEEKNTVEVHIAAFRTFEEMGLLVAAVTSASDYMSPVYHLAGLLAAI
                     GGGSILLMLLTTLVFAKSLTKPMVQIAADLDGASSALLLASSEVTHASQTLSRNASEQ
                     AASMQETTASLHDIVSGGKTVLTAAEAMHSIVDDNRRLGSTSSERVSRLEEEIQRVTE
                     SGSRISQVMTSIDEIAFQTNLLALNAAIEAARAGHNGAGFAVVADEVRRLSQRSTEAA
                     ASSQRELTETFNRITSMGAMVEAIRSSFDALTGSSQILNERLIQVTQAVSEQQIRLNE
                     ISKAAADVDRNTQDIAAISEETAASSFGMAEQARTVSRHAARLRAIVSGLPKSGTPAD
                     PSPQQTAPEIEKTLPPDEPGKPGD"
     gene            5811..6077
                     /locus_tag="PLD82_02890"
     CDS             5811..6077
                     /locus_tag="PLD82_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG64368.1"
                     /translation="MTVKTIYDADYRKILIQLKRAREERGLSQMEAARLLNMTQSTLS
                     KCENGDRRIDIIELTRFAAAYEKDMEYLLPENIRNILLPKIDHR"
     gene            complement(6087..>6748)
                     /locus_tag="PLD82_02895"
     CDS             complement(6087..>6748)
                     /locus_tag="PLD82_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOG64369.1"
                     /translation="ISPNMATMLAFVITDADISTKKLQKYVLDNIEDSFNAVTVDGDM
                     STNDSLVVLANGASQTTLQDKEERRAFKKALFEVMESLALQVVRDGEGATKCIEILVE
                     GAKSRKDAEITARAIANSLLVKTAFFGNDPNWGRIIDVAGYSGADVRENRMELFYLDR
                     VIFSRGKPLPFDKKELSSKLAATKDIKIRLSLGEGRYSRRLYTTDLSYDYVKINAEYT
                     T"
BASE COUNT         1571 a         2007 c         1769 g         1401 t
ORIGIN      
        1 gatgaagttc ccgctctcgt caaaaagctg cagcctcccg ttcccgctgt ccgacacaag
       61 aatccgcccg tcttcgagga cgagaacatc cgacgggcgg aaaaactccc cttctccacg
      121 ccccttgcgt ccgaatgaca tcaggtattt tccctgggaa gagaatttct ggatccgatt
      181 gtttccggaa tcggcaacgt agagcgcacc cgtcttgtcg acatggatgc cttctgggcc
      241 agccaattct gaagcccgat atccgagccg gccgatgatg gccagttttt gtccgcttcg
      301 tgaatagcgg acaacagaat cgtttccgaa atcggaaaca taaaaactcc cgtcgggagc
      361 gaaggccaga tcgtatggct tggagagacc ggccatgaga acattgagaa gattcccgtt
      421 gggatcgaga atcgagactt tcccgcttgc aaagccggca atatagaggt tgcggttttc
      481 gtcaatggcg agtccactcg gccggacaaa caggcttttt ttgtccctgt acccgtcaaa
      541 ccctttcagg aagatccatg gtgacgcctc gtcataggca tcctcacggg tttcgatggc
      601 ataaacattc ttcagcttgt tccgaagaag gctcccgcct gctccaaggt tgagagcgac
      661 ctgccattcc gtcatggcat ttttctggaa accggccatg taaaaagact gaccgagcca
      721 gattcgggaa cgatgatcgt gcgggttgac ggaaatcgcc tgatcgaaaa aagaaaccgc
      781 ggccgtgtat tcccgtcgat tgtagaagta gtacccgctg cggaaattct tctcgactgc
      841 cagactctcg ctgagctgca aggcatcagc ctgggcttga accaaacccg aaggtatcac
      901 aagaaagacc gcgtaccaag ccaaaaaaca attcttcatt cgcataccgg accagccgga
      961 agagagaaac tgcctgcgtc aggtttttcc cccaagaagc ataccgcgca tcttcgaggc
     1021 atcgtgaacc atcgcggaca gaagttcttc ggaaccccgt gtcgttccaa gcaggagcag
     1081 gaactgctgc aattcctcgc ggctaatcac ctgcgggggt gcgaccgttg ccttttgagc
     1141 ggtctccggg cgttcttcct caacccgttc gctctcaacg gcttcgaccc ggcccgcacg
     1201 atccttgcgt gactgccccg catatggtcg ctgcagagca ggttgttgat gggtaacatc
     1261 gagacggaca tccatttccg ccacctcctt gtcccgagag gtatccaccg ataccggacc
     1321 ctctcacggc cattatcggt atacctgctc aacaacttga tcctggccca tccccaagac
     1381 aacacgtaaa tgttccttga tctcctcgtt ttcaggagcc agttcgagcg cccgtttgag
     1441 agtctctctt gccagcgtct ccttcccttt tttgtaatag atccacccca tggtgtcgag
     1501 aaaagcataa ttccgtggtt cggccttgga ggccttcatc gcctccatca gggccctgtc
     1561 cagatcccct ccaccctcgc agtacacgta ggccaaggaa ttccgggcgt tgtggttgtc
     1621 gggattgagt ttgagcgctt gctgcaacat ctggagggat ttttcgatct cgcccctgac
     1681 gaagtagaca tgcccgaggg cggaacaggc cgcatcattg ttgaagttga agcgcagaac
     1741 ccggagaaaa tactgttccg cgtcctccca aacctcacga cgcacagcaa tcaccccgag
     1801 cagcatacag gcctgctgga catggagata gccgtattcg gaatgcacaa cccgttcgag
     1861 atgaggcaca gcctccccgt actcccccgt cttggcaaga gcgatgccca gaagatactg
     1921 ggcctcgaga ttctccggat cgagcttttc aatcttgacg agttccttga tggaactctt
     1981 gaccctgttt tcgtcaagat ccttgcgggc taccgcaaga tagccgtcaa tatccttttg
     2041 ccggatcacg cttcccctcc ccaaggtact ccggttccgt taccgcaaac agtgtagcac
     2101 aacttcgaca ccgcatgcaa ggttatacta caggatcggc ctcacgcccg acaccccgtc
     2161 cggccagaga ttccagactc cggtaatccg tggcatccag aagaacaggg gtcagggaga
     2221 catatccagc atggaccgca tccagatccc cgtcttcgca gggcgtaaaa ctcatcccgt
     2281 cacaactcag ccaaaaatag gtctgcccaa agggcgtcat tctccggtca agaccgtcgc
     2341 gatagacacg ctttcccaga cgagtcacct tgacaccgcg gattccggtt tcaggaagat
     2401 ccgggacgtt gacgttgagc aaggttcccg caggcaatgg cctctcaaga agcacccgag
     2461 cctgttcaag agcgaccttg gcggcaaacc ggtagtcggc agcctcatcc ttgctcgaga
     2521 gcgaaaccgc cataccctgg agcccattga tgatcgcctc acgggcagca gccaccgtgc
     2581 cggagtaata cacgtcgaca cccatgttct gaccgctgtt gatccccgag accacgagat
     2641 cgacgggggt ctcgagaaaa aaatgtccaa gtttgacaca atccgccggt gtcccgtcaa
     2701 tggcgtaccg ggccggaccg tgttcactca accggagagg cgaccagaga ttgacaaaat
     2761 ggctgttggc gctccgctgt ctgtcgggag caatcacact gacggcaaaa tcaggctggg
     2821 tcttgaacac cctctcgagt tcggcaagac cgggtgcatt gattccatca tcgttgacaa
     2881 gaaggatgtg cttcatgcat tataaagtag acagggatac actacaaggc aatcaccgca
     2941 accacctgac ggcgaaagca agaggtttca tccagaggag tacaacgtgc cgccacacat
     3001 aaagaattcc gaacaaattg cagccatccg caccctggcc ggagaactct ccgagcttct
     3061 ggcctggagt ctcgggcagg tccgggcggg catcaccacg caggccatcg atatgcacat
     3121 cgaaaccagg atgcgcgaga tgggggtccg gggaccatgc aaggggtatc acggtttccc
     3181 gaccgtatcc tgcatttcgg tcaacagcca ggtcacacac ggaatcccag acgggaccat
     3241 actccgtgaa ggagatctcg tcgacatcga tatcgttatc gaacgccacg gctattttgc
     3301 cgacatgtca cggaccatca cggttggcga agcctcccca caggcacaaa aattgatccg
     3361 gacaaccgaa gaatgcctgg cctacgggat ttcggccgca cgcccgggcg ggacgctggg
     3421 ggacatcgga gccgccatcc agaatcacgc cgaaaaaaac ggatactccg ttgtcaggga
     3481 ttactgcgga cacttcatcg gaattgcaat gcacgaggat ccgctcgtcc cgaatttcgg
     3541 aaaaaaagga aggggcacaa agctccaacc cgggatgatc ctctgcatcg aaccgatgat
     3601 caacgaggga agctggcagg tcatcacccg tggctgggac gcccgcaccg cggacggaaa
     3661 actctcgtcg cgctgtgaac acatggtcct gattacaccg acaggtcatg aggtgctgac
     3721 gcggtttctc ccctattgac caagtccttc atcaaaccag ttcttgtacg gctcacggag
     3781 gtcaacacca tgcttgcaca gcgcagtctc tcatcccgaa tgttccttgt tttttctgtg
     3841 cttgttgcga tcatcctcac cggactggtc atagcgggaa catttctcac ctccaacacg
     3901 gtcaccactt ccagccagcg ggaacttgtc cagatcgccg agggcatccg taataccatg
     3961 catgccggct atacgttttc gatcgaacgg attgccgggg tactggacga tttcgaatcc
     4021 cgttctgcaa aacagttctt cgtgaacaag caggcctcgg acatgctcga cgtcacctca
     4081 aactcgggag ccaccgaggg gaaccggaca ctcccgagac tgttgtacgg acaaaacccg
     4141 gttttacaca gtcacgagct ttccgatcgg catgcccgtc tgtcgggagg agtttttgcc
     4201 atctaccagc gtgcagggga tgatcttgta caggtctcga ccagtctccg ccatccggac
     4261 ggaaaacggg caaccggaga gatcctcccc gggaaaggga ttccggccgg taccgtcctc
     4321 tccggaaaag agttttcggg caggttgctg gtgaccggtc gctggtacat ctccattctc
     4381 cgcccgctca tggatcctga cggcagcatc atcggggcgg tcctctgtgg catgcccgcc
     4441 agcaatctca gttcgatccg cagcattgtc cacggactgc agatcggaaa cacaggcatg
     4501 gtcggatacg ccatggtgat tgactccgac gggatcgccg tcatccaccc cagtcacgaa
     4561 tcccggtccc tgcttgacaa caccgatatc cacggctaca agtacatccg ggactggctc
     4621 tccagaaaaa acggaaccgc ccgctttgag accgaagaaa aaaacacggt ggaagtccat
     4681 attgccgcat ttcgcacctt tgaggaaatg ggactgcttg tggcagccgt gaccagtgcc
     4741 agcgattata tgagtccggt ctaccatctg gccggcctgc tggccgcgat cgggggcgga
     4801 tccatcctcc tcatgctgct caccacactc gtttttgcca aatcgctcac caaaccgatg
     4861 gtccagattg cggccgatct ggatggcgcg tcctccgccc tcctgcttgc ttcatcggaa
     4921 gtcacccatg ccagccagac cctttcccgg aatgccagcg agcaggcggc gtccatgcag
     4981 gaaacaacag cctcactgca cgatatcgtt tcaggcggga aaaccgtact gacggcggca
     5041 gaagcgatgc actccatcgt tgacgacaac agaagactcg gctccacgag cagcgagcgg
     5101 gtttcccgcc tcgaggagga gatccagcgt gtgacggaat ccgggtcccg catcagtcag
     5161 gtcatgacga gcatcgatga aattgcattc cagaccaatc tcctggccct gaacgcggcc
     5221 attgaagcgg cacgggcagg ccacaatggc gccggttttg cggttgtcgc tgatgaggtc
     5281 cgacggctct cgcagcgttc cacagaggcg gctgcatcaa gtcagcgtga attgaccgaa
     5341 actttcaaca ggattacttc gatgggagca atggtcgagg ctatccgctc aagcttcgac
     5401 gcactgaccg gaagctcgca gatcctcaat gaacggctca tccaggtgac acaagccgtc
     5461 agtgagcaac agatccgttt gaacgagatt tcaaaggctg ccgccgatgt cgaccggaat
     5521 acccaggata tcgcggccat ctccgaggaa acagcggctt cctccttcgg catggccgaa
     5581 caagcacgga ccgtcagccg gcatgcagcc cgactccggg cgattgtctc cggactgccg
     5641 aaatccggca caccagcgga tccgtcaccg caacaaaccg ctccggagat tgaaaaaacg
     5701 cttccccctg atgaacccgg gaagcccgga gactgagcac gtcccagcac gaggaggcaa
     5761 tcgcattatt cgattttggc atataccggc ttgtgcgata tgcaatagcc atgactgtca
     5821 agaccatcta cgatgcggat taccggaaga tcctcattca gctcaagaga gcccgtgagg
     5881 aacgtggcct gtcgcagatg gaagcggccc gcttgctcaa catgacacaa tcaacccttt
     5941 caaaatgtga aaacggtgac cggcggatcg acatcatcga gctgacccgg tttgcggcgg
     6001 cctatgaaaa ggatatggaa taccttcttc cggagaatat ccggaacatc ctcttgccga
     6061 aaatcgatca cagatgatcc cggacatcag gtcgtgtatt cggcgttgat cttgacataa
     6121 tcataggaga gatcggttgt atacagcctg cgggaatacc gcccttctcc cagcgaaagc
     6181 cggattttga tatcctttgt cgcagcgagt ttggaagaga gttccttttt gtcgaaaggc
     6241 aggggttttc ctcgggagaa aatgaccctg tcgaggtaga agagctccat ccggttttcc
     6301 ctgacatccg ccccgctgta ccccgcaaca tcgatgatgc gtccccagtt cggatcgttt
     6361 ccgaaaaacg cggtcttgac aaggagggaa ttggcgatcg ccctggctgt gatctcggca
     6421 tccttgcggg attttgcccc ttcgacaaga atctcgatac acttggtcgc gccctctcca
     6481 tcccggacga cctggagagc cagggactcc atgacttcaa agagggcttt tttgaaagct
     6541 cttcgctctt ccttgtcctg aagcgtcgtc tggctggcac cgtttgccag aacaaccaga
     6601 ctgtcattgg tgctcatatc cccgtccacg gtgaccgcat taaaactgtc ttcaatattg
     6661 tcgagaacat atttctggag ctttttggtc gagatgtcgg catcggtgat gacaaaggcc
     6721 agcatggtcg ccatgttcgg agagatca
//
LOCUS       DAOSHE010000064         6747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9847_length_6747_cov_3.755529, whole genome shotgun sequence.
ACCESSION   DAOSHE010000064 DAOSHE010000000
VERSION     DAOSHE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9847_length_6747_cov_3.755529"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..993)
                     /locus_tag="PLD82_02900"
     CDS             complement(4..993)
                     /locus_tag="PLD82_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOG64370.1"
                     /translation="MGNMARRSIGAGILLVSLCSAMLVGGLLPHDKRDEVIEEAKRYI
                     GVPYLSGGTTPSGFDCSGYIWYIFRQIGGDVPRITFDQYAKLAVRQEPRPGDLVYFHT
                     AGPGASHVGIYIGDGKFLHSPQTGYTIDYANLNDAYWRANFEGIRSAFVHPDDRDFDT
                     RLISARTRVLGRFAFSDAGLFASGSSPNGLDAIFAVHYGLAVEIPLAPSWTLSPRLLL
                     DFAVVNDAAHTWLPASVRVPLMGRYYLVDGASFGLALEAGPVVGFSLGEYTGTDGYKN
                     FTGTTLDLAIGTSFEFGSWGIDLSYELGLTDTYSSDTASGRLGRFTIGIQFAL"
     gene            1143..2117
                     /locus_tag="PLD82_02905"
     CDS             1143..2117
                     /locus_tag="PLD82_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG64371.1"
                     /translation="MPGSSRIRAVTFMALFLFVMAFLTGCGDDADPLSLSVNPESKDS
                     VTQLYKIVYLPDQTLNPGWTGSVAACNPGTITLAYRQATVRRVNYYRALCQLPTVSLD
                     EDQSAKCQHAALIMSANRSLSHTPPDTWLCWTQDGYDAASHANLALGSCGPPAIGLYM
                     DDYGTGNAAVGHRRWILSPPLAKVGTGSVPAASGYQAADALWVVGNAGDRPATPEYVA
                     WPSAGWFPKQHLPTLEGRWSFSVNGADWSQTSISTTRNGVAVTIRMETVNNAGYGDRT
                     IVWCPTDGTANRFVGPEDVWNISVNNVLINGQSKNYNYTIRVFDPETL"
     gene            2178..2906
                     /locus_tag="PLD82_02910"
     CDS             2178..2906
                     /locus_tag="PLD82_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64372.1"
                     /translation="MANDRSSRVPEASPEALAVQQQAVFSAMTEMIVLHQVIFDQEGK
                     PVDYRITDCNSAFSKITGISRDAAIGRLASEVYQVRPAPYLEVYSETGMTGTPTQFET
                     YFPPMDKYFMISVVSHAPNCFATITTDITDIKRLQLQVEGKNRELEQIVYIASHDLRS
                     PLVNVDGYSRELAFALDDLEKTLVEESHPPNESVSTILTTLAEMRTALRYIRNSTTQM
                     DALLKGLLKLSRSGRAALTIAPLI"
     gene            2915..3352
                     /locus_tag="PLD82_02915"
     CDS             2915..3352
                     /locus_tag="PLD82_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG64373.1"
                     /translation="MEKVLASFAWQIKEAGVRVTVNTLPPCKADGVQLTEVFSNLISN
                     AIKYFSPARPGSITISGISENGRSTYIVEDNGIGIAKHHQANIFEMFHRLDPAATPGD
                     GMGLTLVRQILTRLDGNIRVESEPDKGSRFYVELPAAILKQKE"
     gene            3369..3815
                     /locus_tag="PLD82_02920"
     CDS             3369..3815
                     /locus_tag="PLD82_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64374.1"
                     /translation="MNQEVVILIADDDQGHAELIRRNLVRAGIANPLIHFKDGQEVVD
                     FLTRQGSEPHRKPDTAYVLLLDIRMPKLDGVEVLSIIKQDPELKKIPVIMITTTDDPR
                     EVERCHLLGCSNYITKPVEYEGFVNAIRQLGLFLSVVAVPKISGET"
     gene            3833..5011
                     /locus_tag="PLD82_02925"
     CDS             3833..5011
                     /locus_tag="PLD82_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64375.1"
                     /translation="MNAEHHDRTSPSTTRNILVVEDDEALGLLISRRLMRDGFKAESV
                     TTGERALAIWHPEMAILLDQRLPDMTGHELIEKLHNQGHKPFFIVMTGQGDERLAVEM
                     MKLGADDYLVKTIDFMQILPQAFARLFTRRETEARLEKAEEDRRRLEAQLRQEQRLSA
                     IGTLASGVAHEINNPLNVMINYAQLLLDRLSPREHQLLEFCQEILDEGQRIATIVRNL
                     LSFSRVDQAGHRLESPQHLVEGVLSLIGKLLAKDEIALSVEIQPDLPMIECSGPQIQQ
                     IILNLIINGRDAVNERYFGYHPEKKLVVRVAVQPTEVPCVRFSVTDSGSGIPESIREK
                     IFHPFFTTKDPDKGTGLGLSICQNIAHEHHGVLTFETDEGKGTSFHLDIPSAQGGTGP
                     "
     gene            complement(5100..6218)
                     /locus_tag="PLD82_02930"
     CDS             complement(5100..6218)
                     /locus_tag="PLD82_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64376.1"
                     /translation="MLEIREVLTKRDTMRFIKMPWKVYKGDPNWVPPLIFDMKHTLDR
                     KKHPFFEFGEAAFFLALRDGEVVGRITAHINHKHDEYHKTRDGFFGFYECMQDDEAST
                     ALLQTAETWCRERGATKMIGPMNYTIYDEIGLLAEGHDNEPKTPVILEVYTPKYYIDL
                     MAKAGYVKDIDWLSFMIGKDVVVPELFAKVKDRLVSKGFVFRCIDHKNLAAEVERIKG
                     VVNTAWAANWGHVPYSDRQFEAVTEALKMILDPRMIFLVDKDGETVATSITLPDLNPS
                     VKKMNGQLFPFGWWHFLRAKKKAYGLRTFLFGVKEEYRNRGIDAVLVMDTIDAGRKYG
                     YQWSSCSIVVENNLKMIQPILDWGGYEFKRYRIFTKTL"
     gene            6392..>6747
                     /locus_tag="PLD82_02935"
     CDS             6392..>6747
                     /locus_tag="PLD82_02935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOG64377.1"
                     /translation="MTPAILEKYAAVLEWGLETARGKQYKPYETVLIRYDVDALSLTE
                     ALWRRLISRKLNVTARLLTPPVMEKDFYALSDARQRGFIAAGEREFYENLNGNIYLGA
                     PSSLTHLKEIDPKRIA"
BASE COUNT         1607 a         1970 c         1712 g         1458 t
ORIGIN      
        1 tcctcacagc gcaaactgga tcccgatggt aaagcggccc aggcgaccac tggccgtgtc
       61 gctcgaatag gtgtccgtca ggccaagctc gtacgacaga tcgatccccc agcttccaaa
      121 ctcaaacgag gtcccgatgg ccagatcaag cgtcgtgccg gtaaagtttt tgtacccgtc
      181 agtccccgtg tactccccga gactaaagcc gacaacgggc ccggcctcaa gcgcaagacc
      241 aaaggaagct ccgtccacca gataatagcg ccccatgagc ggcacgcgga ccgaggccgg
      301 cagccaggtg tgggccgcat cattgacgac tgcaaaatcg aggagcagcc ggggtgaaag
      361 cgtccagctt ggggccaggg gaatctctac ggccaaaccg taatgcaccg caaagatggc
      421 atccaggcca ttggggctgg accccgaggc aaagagtccg gcatccgaaa aggcaaaccg
      481 acccaaaacg cgtgtccgtg cgctgatcag ccgggtatca aaatcgcggt catcgggatg
      541 gacaaaggcg ctgcggatcc cctcgaagtt ggcccgccag taggcatcat tgagattggc
      601 atagtcgatc gtgtagccag tttgtggtga gtgcagaaat tttccgtcgc caatatagat
      661 cccgacgtgc gaggcacccg gtcccgctgt atggaagtag acaagatctc cggggcgtgg
      721 ctcctgacgg accgccagtt tggcgtactg gtcaaaggtg atccggggca cgtctcctcc
      781 gatctggcgg aagatgtacc agatgtaccc ggagcagtca aacccggagg gcgtggttcc
      841 tcccgaaaga tagggaaccc cgatatagcg cttggcctcc tcgatcacct catcccgttt
      901 gtcatgtggc aggagtcccc cgaccagcat tgctgaacag agagacacca acaggattcc
      961 tgcgccaata ctccgccttg ccatattccc catatttcaa cctccaggac atacgaaaga
     1021 catacacgtc tcctcccatc tatcaagtgc atgcccattt ttccgcgtcc ggtgatattc
     1081 atccgtaccg tgctacactt gagcgtacat tgttttttgc tgaatagcct caaggaggat
     1141 atatgcccgg ttcatccaga atacgtgcgg ttacatttat ggcgctcttc ctgtttgtca
     1201 tggctttcct cactggatgc ggtgacgatg cagatcctct cagtctctcg gtcaatcccg
     1261 aatcaaaaga ctccgtcacg cagctctaca agatcgtgta ccttcccgat caaactctca
     1321 acccgggctg gacgggcagt gtggccgcct gcaatccggg cacgatcacc ctggcctatc
     1381 gtcaagcaac tgtgcgtcgc gtcaattatt accgggcact ctgtcaactg ccgaccgtgt
     1441 cacttgatga agaccagtcg gccaagtgcc agcatgccgc gctgatcatg tcagccaatc
     1501 gctccctcag ccacaccccg cccgatacct ggctctgctg gacgcaggat ggctatgatg
     1561 ccgccagcca cgccaatctc gccctcggat cctgtgggcc tccagcgatc ggactctata
     1621 tggacgatta cgggacaggg aacgccgcag tcggacaccg acgctggatc ctctccccgc
     1681 ctctcgccaa agtcgggaca ggctccgtcc ctgccgcctc cggctatcag gcggccgatg
     1741 ccctctgggt ggtcggtaat gcaggagacc gacctgccac ccccgaatat gtagcctggc
     1801 cttctgcagg gtggttcccc aagcaacatc tccccaccct tgaaggccgc tggtcctttt
     1861 cggtcaatgg cgcggattgg tcacaaacca gtatcagcac aacccgaaat ggtgtagctg
     1921 tcaccattcg catggagacc gttaataatg caggctatgg ggaccggacc atcgtctggt
     1981 gccccactga cggaaccgcc aaccgctttg tcggtcccga agacgtctgg aatatctctg
     2041 tcaacaacgt cctcatcaat ggccaaagca agaactacaa ctacacgatt cgggtctttg
     2101 atcctgagac actctagtca cttcatcatt gaaatatttc gattttagga gatactgttt
     2161 ggagaaagag gtttcgaatg gcaaatgatc gctcatcccg ggtgcccgaa gccagccctg
     2221 aagcccttgc cgtccagcag caggccgtct tcagcgccat gacagagatg atcgtcctcc
     2281 atcaggtcat ctttgatcaa gagggaaaac cggttgatta ccgtatcaca gattgcaata
     2341 gtgcgttttc aaaaatcacc ggaatatccc gcgacgctgc catcggcagg ctggcgagtg
     2401 aggtctacca ggttcgtccg gccccctatc tcgaagtcta ttctgaaacc ggaatgaccg
     2461 ggacaccaac ccagttcgaa acctactttc ccccgatgga caagtatttc atgatctcgg
     2521 tcgtctcgca tgccccaaac tgctttgcaa cgatcacaac agacatcact gacatcaagc
     2581 gtcttcagct ccaagtcgaa ggaaaaaatc gcgaacttga gcaaatcgtc tatatcgcct
     2641 cacacgatct gcgctcaccc ctggtcaatg ttgacgggta ttcacgagag ctggccttcg
     2701 ctcttgatga tctcgaaaag actcttgtcg aagagtctca cccccccaat gaatccgtgt
     2761 ccacaatcct caccacactg gcagagatgc gaacagccct tcgctacatt cgaaacagca
     2821 ccacccagat ggacgccctc ctcaagggcc tcctcaaact ctcccgctct ggtcgtgcag
     2881 cgttgaccat tgcccccttg atatgaatac cttgatggaa aaagtgttgg cctcgtttgc
     2941 ctggcagata aaggaagccg gtgtcagggt cacagtgaac acacttccgc cctgtaaagc
     3001 ggacggtgta caactgacgg aggtgttttc caacctgatt tccaatgcca tcaaatactt
     3061 ttctccggca cgaccgggat cgatcacgat ctccggaata tccgaaaacg gccgttcgac
     3121 ctacatcgtt gaagacaatg gtatcgggat tgccaaacat catcaggcaa acatcttcga
     3181 gatgtttcat cggcttgacc ctgccgcaac accgggtgat ggcatgggcc tgacccttgt
     3241 acgccagatc ctcaccagac tcgacgggaa catcagagtg gagtccgaac ccgacaaggg
     3301 aagccggttt tatgtcgagc ttccggccgc gatactgaag cagaaagaat gacatgaagg
     3361 agcacaccat gaaccaggaa gttgtcatct tgatagcaga cgatgaccag gggcacgccg
     3421 aattgatccg gcgcaatctg gtcagggcag gtatcgccaa ccccttgatc catttcaagg
     3481 acggacagga ggttgtcgat tttctcaccc ggcaggggtc ggagccgcac cgcaaacctg
     3541 ataccgccta tgttctgctc ctcgatatcc gcatgccgaa actggatggt gtcgaggttc
     3601 tttcaatcat caaacaggac cccgaactga aaaagattcc cgtcatcatg atcacgacca
     3661 ccgatgatcc ccgggaagtg gagcgctgcc accttttggg atgcagcaat tacatcacca
     3721 agccggtcga gtatgaggga tttgtcaacg cgatccgtca actcgggctc tttctctcgg
     3781 ttgtggccgt cccaaaaata tccggcgaaa cctgactctg gcggacatct ccatgaatgc
     3841 tgaacaccac gaccgtacat ccccatccac aacccgaaac atcctggttg tcgaagatga
     3901 tgaagccttg ggtctcctga tatcccgcag actcatgagg gatggattca aggccgagtc
     3961 ggtcaccacc ggagaacgtg ctcttgccat ctggcacccg gagatggcca ttcttctcga
     4021 tcagcgattg ccggacatga cagggcacga attgattgaa aaactgcaca accaggggca
     4081 caaaccattt tttatcgtca tgacagggca gggtgatgaa cgtctggctg tcgagatgat
     4141 gaaactcggg gccgatgatt atctggtcaa aacgatcgat ttcatgcaga tcctgccgca
     4201 ggccttcgcc agactcttta cccggagaga gacggaggca cggcttgaaa aagccgagga
     4261 ggaccgcagg cgacttgaag cccaactccg acaggaacag agactctcgg cgatcgggac
     4321 tctggccagc ggggttgccc atgaaatcaa caatcccttg aatgtcatga tcaattacgc
     4381 gcaacttttg ctcgacagac tttcgcccag agaacaccag ctcctcgagt tctgtcagga
     4441 gattcttgat gagggtcagc ggatagccac aatcgtacgc aatcttcttt ccttttccag
     4501 ggtggatcag gcaggccatc gcctggaatc accccaacat cttgttgaag gcgtgctgtc
     4561 cttgatcggt aaactcctgg ccaaggacga gattgcgctt tccgtcgaga tccaaccgga
     4621 cctgcccatg atcgagtgtt ccggaccaca gatccagcag atcatcctca atctcatcat
     4681 caacggaaga gacgctgtca acgagcgcta tttcggctac catcccgaaa agaagctcgt
     4741 cgtccgtgtt gctgtacagc caacggaagt accctgtgtc cggttcagtg tgacggattc
     4801 aggatcggga atcccggaat ccatccggga aaagatcttt cacccgtttt ttaccacaaa
     4861 ggatcccgac aaggggaccg gtcttggcct ttctatctgt caaaacattg ctcatgaaca
     4921 ccatggtgtc cttacattcg aaacggatga agggaagggg acgagtttcc atctcgatat
     4981 accgtcagca cagggcggga caggaccctg acggccagga aaacggtctc cggccgaaaa
     5041 ccgttgatgc agtccagccg ccatctctgt gggcagacag atatttttca gccgatacat
     5101 cagagtgtct tggtaaaaat ccggtagcgt ttgaactcgt atccgcccca gtccagaatc
     5161 ggctggatca tcttgaggtt gttttcaacg acaattgaac aagacgacca ctgatacccg
     5221 tatttccgtc cggcatcaat cgtatccatg accaggactg catcaatccc gcgatttcgg
     5281 tactcttcct tgactccaaa gaggaatgtc ctcagtccat aggccttttt tttggctcgc
     5341 aaaaaatgcc accagccaaa gggaaagagc tggccgttca tcttcttgac cgaaggattc
     5401 agatcgggca gggtgatgga ggtggcgact gtctccccgt ccttgtccac cagaaaaatc
     5461 atccgtggat caaggatcat cttgagggcc tcggtcacgg cttcaaactg tctgtcggag
     5521 taagggacat gcccccaatt ggcggcccag gccgtattga cgacgccctt gatccgctcg
     5581 acctcggccg caaggttctt gtggtcgata caacggaaga caaacccttt ggagacgaga
     5641 cgatccttga ccttggcaaa gagttccggg acaacaacgt ccttcccgat catgaaggag
     5701 agccagtcga tatccttgac gtacccggcc ttggccatca ggtcgatata gtatttgggc
     5761 gtgtacacct caaggatgac gggcgtcttc ggttcgttgt catgcccttc agccagaaga
     5821 ccgatttcat cgtaaatggt atagttcatc ggcccgatca tcttggtcgc accacgctcc
     5881 cggcaccagg tttccgcggt ctggagcagg gccgtcgagg cttcgtcgtc ctgcatgcac
     5941 tcgtaaaacc cgaaaaagcc gtcgcgggtt ttgtgatact catcgtgctt gtggttgatg
     6001 tgcgcggtga tccggccgac aacctcgccg tccctgagag ccagaaaaaa cgccgcctcg
     6061 ccaaactcga aaaatggatg ctttttccgg tcaagagtgt gcttcatgtc gaaaatcaag
     6121 ggtggcaccc aattgggatc gcccttgtat accttccatg gcattttgat aaaccgcatc
     6181 gtgtcacgtt tggtcagtac ttcgcgaatc tcgagcatga tctggctcct cggaaatctc
     6241 gtttgtgtat cccgctcttg caaaggtacc aatgtttttt ggggtcctca agcgcatcca
     6301 gtatatacta tttatggaac aagttccagt ccggagatat ctgccgggac tggatggatt
     6361 tcaacaaacc aatacacccg caggtaccac catgacacca gccattctag aaaaatatgc
     6421 tgccgtcctc gaatggggac tcgaaaccgc acggggaaaa cagtacaagc cctatgaaac
     6481 ggttctcatc cggtacgatg ttgacgcgct ttccctgacg gaggcgctct ggcgacgctt
     6541 gatctcgcgc aaactcaacg tcacagcccg cctgctgacg cctcccgtga tggaaaaaga
     6601 cttttacgca ctctccgatg ccaggcaacg cggattcatt gccgcgggcg agcgtgaatt
     6661 ttatgaaaac ctcaacggga atatctatct cggggcaccg tcatccctga cccatctcaa
     6721 ggagatcgat cccaagcgga ttgccga
//
LOCUS       DAOSHE010000065         6740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9863_length_6740_cov_3.808527, whole genome shotgun sequence.
ACCESSION   DAOSHE010000065 DAOSHE010000000
VERSION     DAOSHE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9863_length_6740_cov_3.808527"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="PLD82_02940"
     CDS             complement(<1..1790)
                     /locus_tag="PLD82_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG64378.1"
                     /translation="MRVALVFPPLCDPTAPYIALPVLAAVLRDRGHEVLMVDANLEAA
                     RAVLSWTFAERARMRIEHRLAKLEKEPVLNHRNAVRYAALWKGRGEGLNLAARLESVV
                     ELLTGKRSGFYTNERYAEAVALAERAMALQSAAWAPLAMDFRQYRTPLAMMDRASIER
                     DAEPARNPFSGFFTGIAERIKEWGAECVSLTVAFPGQLLPSQIMAARLREVMPDVLLL
                     GGGPAISQFFFGMPGDAIREGLGPFDAVVAGDGDEALPRIVEALARGDRAQGFLFEEQ
                     RADMTRQPCPDFEGLPLEDYLSPKLVLPYDLARGCYWGRCAFCHYGTVPGGTAPYRVR
                     DPAVAAAHLVELKARYGAAVFYLSEDALFPAHAREFARNLRGKDVRWSTDLRPEHGFT
                     ASLAREMVAGGLLSVSVGIESASPRTLVRMDKGIDHDVMRTAVHHLAEAGIAVEAMAF
                     EGFPGESARDALLTLRFLAEEYENFSLFIFGQFGLVRGSRIFASPADYGINEIWSVAG
                     DRFASGLFWSGKARGYTDQESAQIESALEDLSAGYRLAQYPWAGSLSTAHTQLRYLAD
                     GPRAFACQADLPSAPARTRESRVFPSRFDVG"
     gene            complement(1907..3562)
                     /gene="pgi"
                     /locus_tag="PLD82_02945"
     CDS             complement(1907..3562)
                     /gene="pgi"
                     /locus_tag="PLD82_02945"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG64379.1"
                     /translation="MLSSVKPVSTGAWKRLEAARQRLETVHLKDLFAQDARRFDRYSL
                     QFGDILIDYSKNILDDEARSALVELACEMDLAGAIEAMFSGKPINVTENRAVLHTALR
                     NRSDRPVMVDGRDVMPDVRAVLAKMKDFSGRVLSGEWKGYSGKRITDIVNIGIGGSDL
                     GPVMVTEALKHYARPGISVHFVSNVDGTHIAETLKRLDPETALFMIASKTFTTQETMA
                     NAHSARDWFVRAAGDVAHVSKHFVAISTNESEVKKFGIDPANMFGFWDWVGGRYSLWS
                     AIGLSIACSIGYDAFEELLTGAYEMDEHFRTTPFESNIPVILALLGIWYNNFFGAHSH
                     AILPYDQYLHRFAAYFQQGDMESNGKSVGRDGEPVTWQTGPIIWGEPGTNGQHAFYQL
                     IHQGTKLIPCDFLAPAVSLNPLGEHHKLLLSNFFAQTEALMNGKTLQEVEQELAAAGM
                     DAAAIAGLAPFRVFAGNKPTNSILFTKMTPRVLGTLVAMYEHKIFVQGILWNIYSFDQ
                     WGVELGKQLAKKILPELDGAGTVSSHDSSTNGLINAWKRMKNT"
     gene            complement(3769..5721)
                     /locus_tag="PLD82_02950"
     CDS             complement(3769..5721)
                     /locus_tag="PLD82_02950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG64380.1"
                     /translation="MSNTTFERFSDLDVRPDIRDALARSGFVTPTPIQKLVLPDALAG
                     RDVFGQAQTGTGKTLAFAIPIAQKVDPAARYVQAVVLVPTRELAKQVANEIEKVTVTT
                     KGLRCVAIYGGVGQQPQVDALSSGAQVVIGTPGRVVDLMRQKELKLDRVSIAVLDEVD
                     EMLDMGFYDEVHTVMTATPKTRQTMFFSATIPPRIQKMGRSYLRREVIHTTSEANLLV
                     DSTEHAFYEVPGRERLEGLMRLIDCEDISKAIIFTNTKIEADRVCVALARVGYPVEGL
                     HGDLPQAKRESVMARFREGKTAIVVATDVAARGLDIRDVTHVIHYHIPLDPETFVHRT
                     GRTGRAGASGKSIMIVSPAEYHDLFKIQEANHLAIQNYPLPSEEAAFAKRMEKMFDKI
                     LRGLASEDISTVYKEMKKHIPLFKRGKALAWLVKKCSEGGFRISESGSAPVRGVAAAV
                     QGPEARLFVNIGRRHGTNESQLREFLAKEAGIKETQVGRIDAKDGYSHVSVESGVAET
                     IISKASGKKMGRFVVKIEIAKSRESERGERGDRGDRGGRDGRRERDGRRDERGPRSSR
                     PARPERAPRQERDSAAEGESTPASDGTQGALPREGSGVRVRRRGGRGGRGREQRQSEA
                     GQTGGSEESSSENRSEVEFRDDTSEN"
     gene            complement(5714..>6740)
                     /locus_tag="PLD82_02955"
     CDS             complement(5714..>6740)
                     /locus_tag="PLD82_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64381.1"
                     /translation="IQSRLLVEDTGYFYLEGLDDWYRQTGFAAERDSLLVRCLDYDEV
                     RYAVVRLEEPVLDGFVGSRMRTSICTWLVSQVKREPGRFPGTLPNGFSISGALRFLLH
                     ARGSFFERYPCNLSFFMSLDERFLVTGNQFILRSESQTEDFVEHYIGQESSLLFEPQD
                     IARFNRALEALFELGDAATARDLFMQLAAEYPQEMLLHKYIYQAAWHLEDFEAVRRHV
                     AIYLERFPRDPDAVCTLAEVAMFEGEYDKSAELLAQAETLVHPNDKTMLADVCIVRMR
                     LLWETHDDEGAARVAQRLLELEPDNEEALALLEDHPLPEGKTKRAGVIKADFARKKTI
                     ETGEQDE"
BASE COUNT         1374 a         2126 c         1949 g         1291 t
ORIGIN      
        1 cccacatcaa accgtgacgg gaagacccgg gactcccgtg tccgggccgg agccgagggc
       61 agatccgcct gacaggcaaa ggcccgcgga ccgtccgcca gatagcggag ctgggtgtgc
      121 gccgtcgaga gggagcccgc ccaagggtat tgtgccagcc gatatccggc tgaaagatcc
      181 tcgagggcgg actcgatctg ggcggattcc tggtcggtgt agccccgggc cttccccgac
      241 caaaacaacc cgctggcgaa ccggtcaccc gctacactcc agatctcgtt gattccgtag
      301 tccgccggag aagcaaagat cctcgatccc ctgaccagtc cgaactggcc gaatatgaag
      361 agcgaaaagt tctcgtactc ctccgcaaga aaccgcagcg tcaggagggc atcccgggcg
      421 gactcgccgg gaaagccttc gaaggccatg gcttcgacgg caattccggc ctctgccagg
      481 tggtgcacgg cggtccgcat gacatcatgg tcgatcccct tgtccatccg aacaagagtc
      541 cgcggtgaag cactctcgat cccgacggag accgagagga gtccgcccgc gaccatctcg
      601 cgcgcaagac tggccgtaaa accgtgctcc ggccgcagat cggtcgacca gcgcacgtcc
      661 ttgccacgaa ggttgcgggc aaactcacgg gcatgcgccg gaaagagcgc gtcctccgac
      721 aggtaaaaga ccgccgcccc atagcgggct ttgagttcca ccagatgggc cgcagccaca
      781 gctggatcac gcacccggta gggtgctgtc cctccgggaa ccgtaccgta atgacagaag
      841 gcgcaccggc cccagtagca gccgcgtgcc aggtcatacg gcaggaccag tttgggcgag
      901 agatagtctt ccagcggaag cccttcaaaa tccgggcaag gctgccgtgt catgtccgca
      961 cgctgttcct caaaaagaaa cccttgcgca cgatcacccc ttgcgagtgc ttccacgatc
     1021 cgtggcaggg cctcatcccc atccccggcc acaacggcat cgaagggacc cagcccttcc
     1081 cggatggcgt ccccgggcat cccgaaaaaa aactggctga tggccggacc accacccaag
     1141 agaaggacat ccggcatgac ttcgcgcaac ctggctgcca tgatctggga agggagcaat
     1201 tgtccgggga atgcgaccgt cagcgaaaca cactccgcac cccactcctt gatccgctcg
     1261 gcaatcccgg taaagaaccc agaaaaggga ttgcgtgccg gctcggcatc ccgttcgatc
     1321 gaggcccggt ccatcatggc cagcggggtt ctgtactgac ggaagtccat ggccagcggg
     1381 gcccaggcgg cactctgcag agccatggcc cgttctgcca gggctacggc ctcggcatag
     1441 cgctcgttgg tatagaaccc gctcctcttg ccggtcagga gttcgacaac cgactcgagt
     1501 ctggcggcca ggttgagtcc ctcaccccgc cccttccaga gggcagcgta acggacggca
     1561 ttccgatggt tgagaacagg ttccttctcg agtttggcca acctgtgttc gatccgcatt
     1621 ctggcgcgtt cggcaaaggt ccaggacaga accgccctgg cggcttcaag attggcatcc
     1681 accatcagca cttcgtgccc ccggtcacgc aggacagctg ccagaacggg aagcgcgata
     1741 tagggcgctg tcggatcgca aagaggggga aaaacgagag ccacgcgcat acgaatccct
     1801 gttgacaacg acaccgcccc cggtggtccg ggaagcggtg tcctgagtag tactgaccat
     1861 tatatgtgaa ttttgttatt tgtacagtcc catgaaggaa caaagttcac gtatttttca
     1921 tcctcttcca cgcattgatc aggccgttgg tggacgaatc gtgggaagaa accgttcccg
     1981 ccccgtccag ttcggggaga atttttttgg cgagctgctt gccaagctcg accccccact
     2041 gatcgaaact gtagatgttc cagagaatac cctggacaaa gatcttgtgc tcgtacatgg
     2101 cgaccagtgt acccagcacg cgcggcgtca ttttggtaaa gagaatcgaa ttggtcggct
     2161 tgttgccggc aaagaccctg aagggggcca gtccggcgat cgccgcggca tccataccgg
     2221 ccgcggccag ctcctgctca acctcctgga gggtcttgcc gttcatcagg gcctcggtct
     2281 gggcgaaaaa gttggaaagg agaagcttgt ggtgttcgcc aagggggttg aggctgacgg
     2341 cgggagccag aaaatcacag ggaatcagtt tggtcccctg atggatcaac tggtaaaagg
     2401 cgtgctggcc attggtcccc ggctcgcccc agatgatcgg ccccgtctgc caggtgaccg
     2461 gctccccgtc gcggcccacc gacttgccgt tgctctccat gtcaccctgc tggaaatagg
     2521 ccgcaaagcg gtgcaggtac tggtcgtagg gcaggatggc gtgcgagtgg gcgccaaaga
     2581 agttgttgta ccatatcccg aggagtgcca ggatgaccgg aatgttggac tcgaagggcg
     2641 tggtgcggaa atgctcgtcc atctcgtagg cccctgtcag gagctcctcg aaggcgtcgt
     2701 atccgatcga acaggcgatg gagagtccga tggccgacca gagtgaatag cggccgccga
     2761 cccagtccca gaacccgaac atgttggccg gatcgatccc gaattttttc acctcgctct
     2821 cgttggtgga gatggccaca aaatgcttcg agacatgcgc cacgtccccg gccgccctga
     2881 caaaccagtc acgggccgag tgggcattgg ccatcgtctc ctgtgtcgtg aaagtcttgg
     2941 aggcgatcat gaagagcgcg gtctcgggat caaggcgctt gagagtctcg gcgatatggg
     3001 tcccgtcaac gttggagaca aagtggacgc tgatgcccgg ccgggcatag tgcttgaggg
     3061 cctcggtcac catgaccggt ccgagatccg aaccgccgat cccgatattg acgatgtccg
     3121 tgatccgttt tccgctgtag cccttccact caccggagag gaccctcccc gaaaaatcct
     3181 tcatctttgc caggacggca cgcacatcgg gcatcacatc ccggccgtcc accatgaccg
     3241 gccggtccga acggttgcgc agggcggtat gcaggactgc ccggttttcg gtgacattga
     3301 tcggcttgcc ggaaaacatg gcttcgatgg caccggccag atccatctcg caggcgagct
     3361 cgaccaatgc cgaacgcgcc tcgtcatcga ggatgttttt ggaataatca atgaggatat
     3421 ccccgaactg gagcgagtag cggtcaaagc ggcgggcatc ttgtgcaaaa agatctttga
     3481 ggtgaacagt ctcaagccgc tggcgggctg cttcaaggcg tttccaggct ccagtggaca
     3541 caggtttgac ggaagaaagc atgtgtgcat actccttgtg agaggtatgc gtcagtatgc
     3601 cccgtccgga gtcggctgtc aaccgccctc aagaagttcc ggatgccggc gcaaggtccc
     3661 gcacgaaagc agcacaggca gcaatggact atgccccgca ggaagaaaaa ccgagagact
     3721 gcccggtggc agcccctcgc tcaagacatg ttcctgtatc cgcaggtttc agttttcgga
     3781 cgtatcgtcc ctgaactcga cctcgctcct gttctcggac gaagactctt cactgccgcc
     3841 ggtctgcccg gcctcggact ggcgctgttc gcgtccgcgt ccgccgcggc caccacggcg
     3901 gcgcacacgc acgccgctac cctcgcgggg aagcgcaccc tgtgtcccgt cggaagccgg
     3961 cgtgctctcg ccctcggcgg ccgaatccct ctcctgcctc ggtgcgcgct cgggccgggc
     4021 cggcctgctg ctccttggtc cgcgctcatc acggcgacca tcccgttcac ggcgtccgtc
     4081 gcgaccgccc ctgtctccgc gatctccgcg ttccccccgc tcggactcac gggacttggc
     4141 gatctctatc ttgaccacaa accggcccat cttctttcct gaagccttgc taatgatcgt
     4201 ctcggccaca cccgactcga cggaaacatg gctgtacccg tccttggcgt cgatgcgtcc
     4261 gacctgggtt tccttgattc cggcctcttt cgcgagaaac tcgcgcagct gggactcatt
     4321 cgttccatga cgccggccaa tgttcacaaa aagcctggcc tcgggacctt ggacagcggc
     4381 tgccacacca cggaccggag ccgaacccga ctcgctgatc cggaacccgc cctcgctgca
     4441 ttttttgacc agccaagcca gggccttgcc ccgcttgaag agcgggatat gctttttcat
     4501 ttctttatag actgtgctga tgtcctcgct ggcgaggccc ctgagaatct tgtcgaacat
     4561 tttttccatc ctttttgcaa atgccgcctc ctctgacggg aggggataat tctgtatggc
     4621 gagatgattc gcctcctgga tcttgaagag gtcatgatac tcggcgggac tgacgatcat
     4681 gatgctcttc ccgctggccc cagcccgccc ggtccgtccg gtgcgatgga caaaggtctc
     4741 ggggtcgagc gggatgtgat aatgaatgac atgcgtcaca tcccggatgt ccagaccgcg
     4801 agcggcgaca tcggtggcca cgacaatcgc cgttttcccc tcacggaagc gggccatcac
     4861 cgactcacgc ttggcctggg gaagatctcc atgcagaccc tcgaccggat acccgacgcg
     4921 ggccagggcc acgcagaccc tgtccgcctc gatcttggta ttggtgaaga tgatcgcctt
     4981 ggagatgtcc tcacagtcaa tcagccgcat caacccctca agccgctccc ggccgggaac
     5041 ttcgtaaaac gcatgttccg tcgaatcaac aaggagattc gcctcggagg tggtgtgaat
     5101 cacctcgcgc cggagataac tccgccccat cttctggatc ctggggggaa tcgtcgccga
     5161 aaagaacatg gtctgcctgg tcttgggcgt ggcggtcatg acggtatgga cttcgtcgta
     5221 gaaccccata tcgagcatct cgtccacttc atcaagaacc gcgatgctca cgcgatcaag
     5281 cttcagctcc ttttggcgca tcaggtcgac aacgcgcccc ggagtgccga tcacaacctg
     5341 tgccccgctc gagagcgcat ccacctgggg ctgctgtccc accccgccat agatggccac
     5401 gcagcgcagg ccctttgtcg taaccgtcac cttttcgatt tcattggcca cctgcttggc
     5461 cagttcccgg gtcggtacaa gaacgacggc ctggacatac cgggcggccg gatcgacttt
     5521 ttgcgcaatc ggaatggcaa aggccagcgt cttgccggta cccgtctggg cctgcccgaa
     5581 aacatcccgc ccggccaggg catcgggcag aaccagtttt tgaatgggtg tcggagtgac
     5641 aaacccgctc cgggccaggg catccctgat atcgggacgg acatcgagat ccgaaaaccg
     5701 ttcgaatgtt gtgttactca tcctgttcac ctgtttctat cgtcttcttc ctggcaaaat
     5761 cggccttgat cacaccggcc cgcttcgttt tcccctccgg caggggatgg tcttcgagaa
     5821 gggccagggc ctcctcgttg tccggttcga gctcaaggag ccgctgcgcc acccgggctg
     5881 caccctcgtc atcatgggtc tcccagagaa gacgcatgcg cacgatacag acatccgcca
     5941 gcatggtctt gtcgtttgga tgcacgagag tctcggcctg tgccaggagt tctgccgact
     6001 tgtcatactc cccttcgaac atcgcgacct cggccaaggt acagacggca tcagggtcgc
     6061 ggggaaaccg ttcgagatag atggccacgt gccgccggac agcttcaaaa tcctcaagat
     6121 gccatgccgc ctggtaaata tacttgtgca ggagcatttc ttgggggtat tcggctgcaa
     6181 gctgcatgaa caaatcccgg gccgtcgctg catcccccag ttcgaacaga gcctcgagag
     6241 cccgattgaa ccgggcaatg tcctgcggtt caaacagaag actgctttcc tggccgatgt
     6301 agtgctcgac aaaatcctcg gtctgtgact cactgcgcag aatgaactgg ttccctgtga
     6361 caagaaagcg ctcgtcaagc gacatgaaga acgagagatt gcagggatag cgctcgaaaa
     6421 aactcccccg ggcatgcagc aggaaccgca gggccccgct gatggaaaac ccgttcggca
     6481 aggtcccggg aaacctcccc ggctctcgct tgacctgcga cacaagccag gtacagatcg
     6541 aggtccgcat ccgggaaccg acgaatccgt ccaacaccgg ttcctcaaga cgcacaacgg
     6601 cataccggac ttcgtcgtag tcgagacatc gtacaagaag ggagtcccgc tcggcggcaa
     6661 aaccggtttg gcgataccag tcgtcaagcc cttcaagata aaaatacccg gtatcctcga
     6721 ccagaagtct ggactggatc
//
LOCUS       DAOSHE010000066         6711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9927_length_6711_cov_3.909856, whole genome shotgun sequence.
ACCESSION   DAOSHE010000066 DAOSHE010000000
VERSION     DAOSHE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_6711_cov_3.909856"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLD82_02960"
     CDS             complement(<1..357)
                     /locus_tag="PLD82_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64382.1"
                     /translation="MRTAVRFRLVLGGALCALLAAGLVWGRLVVQDLLLERLSAGMRD
                     SLIILGRTIFGDKPGSIQSVPDLVRSVAGLEGIRVSLIGPSGFVVVDSTIENTNVMEN
                     HGLRSEVRAARSGGWGE"
     gene            complement(354..1118)
                     /locus_tag="PLD82_02965"
     CDS             complement(354..1118)
                     /locus_tag="PLD82_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG64383.1"
                     /translation="MTNEKKLLDDYLQSMQKNDRPGKILVVDDEPDIIEILSWNLGQE
                     GYQVDSAASGLMALSRIGKNRPDLVVLDRMLPGLDGIGVCDALRKDPALRGIPVLMLT
                     ALGGEDDRVSGLDAGADDYLAKPFSMAELKARVRALLRRSQPEEAGPEILVWKVIRYD
                     PVERTLVSGGPEPVVLTPKEGRLLDFFLRNSGRHIPRERIVDYVWEDPPEDPRALDVL
                     VRRLRSRMGSASGLLSTLPGTGYGLVDAREAGEGTT"
     gene            1152..2111
                     /locus_tag="PLD82_02970"
     CDS             1152..2111
                     /locus_tag="PLD82_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG64384.1"
                     /translation="MNIPRQAAHYESVGVQQKEPAPGSVHAMSRILILTVLAILVTSC
                     HRNRNMIIIKGSDTEVNLVSDFAESFKRIRPASRIAVTGGGSGTGIAALINRTADIAN
                     ASRPMKSHERSLAKKNGVEPRTIVIATDGLAIIVHPENPVRWISLPQLAAVFRGEIRN
                     WGELGGPDRRITCYGRQSNSGTFEFFKTVVLGGGDFDASVLQMNGNAQIVASLGQDPG
                     GIGYVGVGYVQGPGQRLRTLPVARDDRSRACLPVEENITNARYPLTRPLYQYINGAPG
                     PELKRFLEFCISSEAARIVRANGFFPLQPEHHRINRASGLTRT"
     gene            2123..3037
                     /gene="pstC"
                     /locus_tag="PLD82_02975"
     CDS             2123..3037
                     /gene="pstC"
                     /locus_tag="PLD82_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOG64385.1"
                     /translation="MPTTQTRNHRIPDLAEVFFRLVGGSVILILLGILLLLVTNAIPF
                     FAGEHTVDFFFSERWNPSSSQPGYGILSLVAGTLLTTLGAMVLAIPLGILTALWLTEI
                     APPRVSQWIKPAIELLSAIPSVVIGFLGITLLTPLMAGLSGRPNGLNAVSGSVLLAIM
                     ALPTIISMTEDALRSVPSALREASLALGASRWETMTRIILPAARSGVFAAIMLGLGRA
                     IGETMTVLMITGNTPVIPGSIFDPVRTLTATIAIELGEVPVGSEHFHALFAVGLALFA
                     ITFFVNLAADLVMRRQTRMLAPSKEKQS"
     gene            3034..3912
                     /gene="pstA"
                     /locus_tag="PLD82_02980"
     CDS             3034..3912
                     /gene="pstA"
                     /locus_tag="PLD82_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG64386.1"
                     /translation="MTTRCRQQLHPMDRTGRILVTLATGLVLSIMILILTDLLIGGAG
                     SLSWEFVSDMPRDRMTAGGILPCIVGTLAVTLVTTLFSIPFGLATAIYLHEYAKDNRS
                     TRLIRLAIRNLAGVPSIVYGIFGVALFVNGLALGTSILSAGLTLGLLTIPWTITASEE
                     ALRSVPLAWREASLALGATRWTTIRRIVLPRAVPGIITGGILGIARAAGETAPLLFTG
                     ASFSLPGLPESVFEPFMALPYHIYTLATQHESISQVRPIAYGTSLVLIGMILGLSLIA
                     IIIRARLRRQHSGAGA"
     gene            4010..4816
                     /gene="pstB"
                     /locus_tag="PLD82_02985"
     CDS             4010..4816
                     /gene="pstB"
                     /locus_tag="PLD82_02985"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008487144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOG64387.1"
                     /translation="MTTVVTKTGSTESDTHAEKTAISVRNFSFHYGSNKVLHDICLSI
                     PDHQVTALIGPSGCGKSTFLRSINRMNDLVPGARAEGEILVGKTSVNQPGTDIVALRR
                     TVGMVFQRSNPFPKSIADNITYGPKLGGITDRLELERIVEETLTQVGLWSEVRDRLQS
                     SALALSGGQQQRLCIARAIAVQPEILLMDEPASALDPIATRRIEELILELKNRYTIVI
                     VTHNMQQAARISDRTAFFYMGEVVEYDTTRVIFTTPRQSRTEDYVTGRFG"
     gene            4843..5532
                     /gene="phoU"
                     /locus_tag="PLD82_02990"
     CDS             4843..5532
                     /gene="phoU"
                     /locus_tag="PLD82_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG64388.1"
                     /translation="MQIHLDQELEVLRKELSIMRTLATEALVHAIQALETGQNDLALR
                     VIQGDREIDLQENKIDGMLLAIVGLKQPVAIDLRFLMAAMKINNDLERVGDKAASIAK
                     ASIEMASRDRIRPLIETIIAMGEHSSMMIRDAFDCFLGKNADHALEVIGMDRTLNTMN
                     HDFYRTLLECLRSSTTDPGTALALYRTATAIERIGDLAKNISEEAIYYIRGEIIRHGT
                     GNPDVGAHRKS"
     gene            complement(5652..>6711)
                     /locus_tag="PLD82_02995"
     CDS             complement(5652..>6711)
                     /locus_tag="PLD82_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64389.1"
                     /translation="TTTKTTTQSKTFTVNKSGAVTIRFVQSGTTAGQVSIDTLSWTSY
                     GTTSSSSSVSSTSSSVSSISSSVSSSSSSVSSSSSSTAATGTIKVGSFNIEIFGTTKI
                     SRANVLYTLAHIATRYDIIAIQEVGSNGTPSESTATTVMDTYIAKINSIAGAGAYSYV
                     RGHQYAFVYKNATITKKASQLYSGSKTFTYTPLVGNFAVKSGNFDFAMLTIHTSPTVA
                     TTEIPALTTAMADVRTLYSEQDVICLGDYNADGSYYSPGTSGGDLAGFSSSTYVTVIK
                     NGTDTTVSPNNSYTYDRMQLYTASTEDYAGVKGVLKFVDYYTISNLEGTSTTAGTEAA
                     LSDHYPIWAEFYTGRDTN"
BASE COUNT         1371 a         2176 c         1862 g         1302 t
ORIGIN      
        1 ctctccccat cccccgcttc gggcggcccg gacctcgctt ctcagtccgt ggttctccat
       61 gacattggtg ttttcgatcg tgctgtcaac caccacgaat ccggatggac cgatcaggct
      121 gacccggata ccttcgagtc cggccacact ccgtaccaga tccgggacag attgtattga
      181 accgggtttg tccccgaaaa tcgtccgccc gaggatgatc agggagtccc gcatcccggc
      241 cgagagccgc tcgagaagca gatcctggac cacaagccgg ccccagacaa gcccggcagc
      301 caggagggca cagagcgctc caccaagcac cagtctgaaa cggacggctg ttctcatgtc
      361 gtcccctctc ccgcttcacg cgcatccaca agtccatacc cggtcccggg cagggtcgaa
      421 agaagtcccg aagcggaccc catccgggac cgcaagcgac gcacgaggac atccagcgcc
      481 cgggggtcct ctggtggatc ctcccagaca tagtccacaa tccgctccct gggaatatgc
      541 cgtccggaat tccgtaaaaa gaagtcaagg aggcggccct ccttcggcgt cagcacgacc
      601 ggctccggac cgccggaaac gagcgtccgt tccacgggat catagcggat caccttccag
      661 acaaggatct cgggcccggc ctcctccggt tggcttcttc tcaggagtgc ccggacgcgg
      721 gccttgagtt ccgccatgga gaacggcttg gccagatagt catcggcccc ggcatccagg
      781 ccgctgacgc gatcgtcctc tcccccgagg gccgtcagca tcaggacagg aatcccgcgc
      841 agggccggat ccttgcggag cgcgtcgcag accccgatcc cgtccagacc gggaagcatc
      901 cggtcgagaa ccaccagatc cggcctgttt ttcccgatcc gggagagcgc catcagcccg
      961 ctcgctgccg aatccacctg atacccttcc tgtccgagat tccacgagag aatctcgatg
     1021 atatccggtt cgtcatcgac aaccaggatc tttccgggcc ggtcgttctt ctgcatggac
     1081 tgaagatagt catccagaag ttttttttca tttgtcacac gtttgtaaca tctcctgcgg
     1141 aaactctctt catgaacatc ccgcgacagg ccgcccatta cgaatccgtg ggtgttcaac
     1201 aaaaagaacc agccccaggg agcgttcatg ccatgtcccg gatcctgatc ctcacagtcc
     1261 tcgcaatcct tgtcacctcc tgccaccgca acaggaacat gatcatcatc aagggttccg
     1321 acaccgaggt caatctcgtc tcggactttg ctgaatcctt caagcgcatc cggccagcca
     1381 gccggatcgc ggtcacaggc ggaggttcgg gaaccggcat cgccgccctg atcaacagga
     1441 ctgccgatat cgccaatgcc tcccgcccga tgaaatccca cgagcgcagc ctggccaaaa
     1501 aaaacggcgt tgaaccccgg acaatcgtga ttgccaccga cgggctggcc atcatcgtcc
     1561 atccggagaa ccctgtccgg tggatctccc tgccccaact ggccgcggtc ttccgcggcg
     1621 aaatccggaa ctggggagaa ctcggcggcc cggacagacg aatcacgtgc tatggaaggc
     1681 agagtaattc cggcaccttc gagtttttca aaaccgtcgt cctgggaggc ggggactttg
     1741 atgcgagcgt cctccagatg aacgggaacg cccagatcgt cgcctccctc ggacaggatc
     1801 cgggcggaat cgggtatgtc ggtgtcggct acgtccaggg acccgggcaa cgcctgcgca
     1861 ccctccccgt tgcccgggat gaccggtccc gggcctgcct gcctgtcgag gagaacatca
     1921 ccaatgcccg ctaccccctg acccggcctc tctaccagta catcaatggc gccccgggac
     1981 cggaactcaa acgctttctc gaattctgca tttcttccga ggccgcgaga atcgtccggg
     2041 ccaacggatt tttcccgctg caacccgagc accaccggat caaccgtgcc tccggcctga
     2101 cccggacctg aaggagttcc gcatgccgac aacacaaacc cgaaaccacc gcatcccgga
     2161 tctggccgaa gtttttttcc gtcttgtcgg cggttcggtc atcctgatcc tgctgggaat
     2221 tctgctcctc ctggtcacca acgcaatccc ctttttcgcc ggagagcaca cggtcgattt
     2281 cttcttcagc gagcgctgga acccgtcatc ctcgcagccg ggatacggaa tcctctccct
     2341 ggtcgcgggg actctcctga ccaccctcgg ggcgatggtc ctggccatcc ccctggggat
     2401 cctgaccgca ctctggctga ccgagatcgc cccgccgcgg gtcagccagt ggatcaagcc
     2461 ggccatcgaa ctcctctcgg cgattccctc ggtggtcatc ggattcctcg gaatcaccct
     2521 cctcaccccg ctcatggccg ggctcagcgg gcggcccaac gggctgaatg ccgtcagcgg
     2581 ctcggtcctg ttggcgatca tggccctgcc cacgatcatc agcatgacgg aagacgcact
     2641 ccgcagtgtc ccctccgccc tgcgcgaggc atccctggcc ctcggagcga gccgctggga
     2701 aacgatgacc cggatcattc tcccggccgc ccgctcgggg gtctttgcgg ccatcatgct
     2761 cggactgggc cgggccatcg gcgaaaccat gaccgtcctg atgatcaccg gcaatactcc
     2821 ggtcattccc ggctccatct ttgatcccgt ccgcaccttg acggccacaa tcgcgatcga
     2881 actgggcgaa gtccccgtcg gttcggaaca cttccacgcc ctcttcgccg tcggtctggc
     2941 gctcttcgcc atcacctttt ttgtcaatct ggcagcggat ctcgtgatgc gccgccagac
     3001 acggatgctc gcaccgagca aggagaagca atcatgacca ctcgctgcag acaacagcta
     3061 catccgatgg accggacagg ccggatcctg gtcaccctgg caacgggact cgtcctctcc
     3121 atcatgatcc tgattctgac agacctcctg atcggtggcg ccggatcact ctcgtgggaa
     3181 tttgtcagcg atatgccccg cgaccggatg actgccggag ggatcctccc ctgcatcgtc
     3241 gggactctgg ccgtgaccct ggtgacaaca ctcttttcca ttcccttcgg tctggcgacg
     3301 gccatctacc tccacgaata cgccaaagac aaccggtcaa cacgcctgat ccgcctggcg
     3361 atccgcaatc tggccggagt cccgtccatc gtgtacggaa tttttggtgt cgcactcttt
     3421 gtcaacggac tcgctctcgg aacctcgatc ctctccgccg gtctgacact gggactcctg
     3481 accatcccct ggacgatcac cgcgagcgag gaagccctgc gcagcgttcc cctggcctgg
     3541 cgcgaagcct cgctcgccct cggagcaacc cgctggacga caatccgccg catcgtcctc
     3601 ccgcgcgctg tccccgggat catcaccggc ggaatcctgg gaattgcccg ggccgccggc
     3661 gagaccgccc cactcctctt caccggggct tcgttttccc tccccggact tccggagagc
     3721 gtcttcgagc ccttcatggc gctcccctac catatctaca ctctcgcgac acagcacgag
     3781 agcatctcgc aagtccgtcc gatcgcctac gggacctcac tggtcctgat cgggatgatc
     3841 ctcggactct cgctgatcgc catcatcatc cgggccagat tgcgcagaca acacagcgga
     3901 gcgggtgcat gaatcatgca gttttttggt gaacactctt ccgcccaacg atggacggtg
     3961 actttcgtgg atatttcacc cacacgtcaa acttgaaagg agtacccgga tgacaacggt
     4021 tgtaaccaaa acaggcagca cagaatcgga cacccatgca gagaaaaccg ccatctcagt
     4081 acgaaacttt tcgtttcatt atggaagcaa caaggtgctg catgatatct gcctctccat
     4141 ccctgatcac caggtcaccg cgctgatcgg cccgtcgggt tgcgggaagt cgaccttcct
     4201 ccgctcgatc aaccgcatga acgatctggt gccgggagca cgggccgaag gcgagatcct
     4261 cgtcggaaaa accagtgtca accagcccgg gacggatatc gtcgcactcc gtcgcaccgt
     4321 cggaatggtc ttccagcgct ccaacccctt ccccaagagt atcgccgaca acatcaccta
     4381 cgggccaaag ctcgggggaa tcacagaccg gctggagctg gagcggattg tcgaggagac
     4441 cctgacccag gtcggtctct ggagcgaggt ccgcgaccgg ctccagtctt cagcactcgc
     4501 gctctcgggc ggacagcagc agcggctctg tatcgcccgg gcgatcgccg tccaaccgga
     4561 gattctcttg atggacgaac cggcttccgc acttgatccg atcgccacac gccgcatcga
     4621 ggagctgatc ctcgagctga agaaccgcta caccatcgtc atcgtgaccc ataacatgca
     4681 gcaggccgca cggatctccg acaggaccgc ctttttctat atgggtgagg ttgtcgaata
     4741 cgatacgacc cgtgtcatct tcacgacccc ccgccagtca cgaaccgaag actatgtcac
     4801 cggccgcttc ggttaagcgc gccgaaagaa aggacaaata cgatgcagat ccatcttgat
     4861 caggaattgg aggtcctgcg caaggaactc tccatcatgc gcaccctcgc cacggaagcc
     4921 cttgtccatg cgatccaggc tctcgaaacc gggcagaacg atctggccct gcgggtcatc
     4981 cagggcgacc gcgaaatcga tctccaggaa aacaagatcg acggcatgct cctggccatc
     5041 gtcggtctca agcagcccgt cgccatcgac ctgagatttt tgatggcagc catgaagatc
     5101 aacaacgatc tcgagagagt gggcgacaag gcagccagta tcgccaaggc atccatcgaa
     5161 atggcctcgc gggacaggat ccggcccttg atcgagacca tcattgccat gggggaacac
     5221 tcctcgatga tgatccgcga tgccttcgac tgctttctcg gaaaaaacgc ggaccatgcc
     5281 ctggaggtga tcgggatgga ccggaccctc aacacgatga atcatgattt ctatcggacg
     5341 cttctggagt gcctccgctc cagtaccaca gacccaggga cagccctggc gctctaccgg
     5401 accgcaacag ccatcgagcg catcggcgac ctggccaaga acatcagcga ggaagccatc
     5461 tactacatcc ggggcgagat catccgtcac ggaaccggca acccggatgt cggtgcgcac
     5521 aggaaatcgt gacgatacaa agtatctcct gccgggtaga gcgtgttctc ccggcaaacc
     5581 cttggtccaa aacaatcagc ccctgcaggc gacctgcagg ggctggagaa gtaccttgtc
     5641 tggattcaga ctcagtttgt gtcacgtccc gtgtaaaact ccgcccagat cggatagtga
     5701 tccgaaaggg ccgcttccgt tccggcagtg gtggacgtgc cctcgagatt gctgatggtg
     5761 tagtagtcga caaacttgag cacacccttg accccggcat agtcctcagt cgaagcggta
     5821 tagagctgca tgcggtcgta ggtgtagctg ttgttgggcg aaaccgtggt atccgtcccg
     5881 tttttgatga ccgtcacgta ggtcgacgag gagaaaccgg ccaagtcacc accggatgtg
     5941 cccgggctgt agtacgagcc atcggcattg tagtcaccca ggcagatcac gtcctgctcg
     6001 ctgtagagcg tgcgcacatc ggccatagcc gttgtcaggg cgggaatctc ggtggtggca
     6061 accgtcgggc tggtatggat ggtcagcatg gcaaagtcga agtttccgct cttgaccgca
     6121 aaattgccga cgaggggggt ataggtaaag gtcttggatc cgctgtagag ctgcgaggcc
     6181 tttttggtga tcgtggcgtt tttatagacg aaggcgtact ggtggccacg gacgtaggaa
     6241 taggcaccgg caccagcgat cgagttgatc ttggcgatgt aggtatccat cactgttgtg
     6301 gctgttgatt cggacggcgt tccgttggaa ccgacttcct ggatagcaat gatgtcgtag
     6361 cgggtggcaa tgtgagccaa tgtatagagg acgttggcgc gtgatatctt ggtcgttcca
     6421 aaaatctcga tgttgaacga accaactttg atcgttccag ttgcagctgt ggaagagctg
     6481 gagctggaaa cggatgaaga gctagaagaa acggatgacg agatggaaga aacggatgac
     6541 gaagtcgaag agactgagga cgagctggag gtcgtgccgt agctcgtcca ggaaagcgtg
     6601 tcaatcgaga cctgaccggc tgtcgtccct gactggacga agcggatcgt gacggctccg
     6661 gacttgttga ccgtaaaggt cttgctctgg gtcgttgtct tggtcgttgt c
//
LOCUS       DAOSHE010000067         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9948_length_6698_cov_3.695619, whole genome shotgun sequence.
ACCESSION   DAOSHE010000067 DAOSHE010000000
VERSION     DAOSHE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9948_length_6698_cov_3.695619"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLD82_03000"
     CDS             complement(<1..181)
                     /locus_tag="PLD82_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64390.1"
                     /translation="MPDMQILGPIIGVAFAVIMIFVGIIVAKKAGGGGYMKKIHDKYA
                     ALAASLGVPPPQQQNG"
     gene            416..856
                     /locus_tag="PLD82_03005"
     CDS             416..856
                     /locus_tag="PLD82_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG64391.1"
                     /translation="MKGIIRWGCLVCSIVLTAFTLAGYDQKQYNEVKAGNENKTYNFF
                     DLSGADLSGMNLEMACFREANLIRANLAKANLSYANLKGAFLNGANLKDANLAYADMR
                     GANFLEADLSGAIIAGAQVRGARFLAAKLSEAQKKYIEENGGEF"
     gene            complement(920..1378)
                     /locus_tag="PLD82_03010"
     CDS             complement(920..1378)
                     /locus_tag="PLD82_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64392.1"
                     /translation="MKLSGALRTLLEFKKHALDVFLALGNHADPDVALSITDLVEEER
                     EILAGIKTCIDRHGTGTPEANKETLDGLLSMEYFREHASQTRLADRDAALDLAIQFEK
                     DALLLFTEIWTLCPGEECHTELQILITMEKKHLLRLLDMRKSTRVEQYSR"
     gene            complement(1441..2427)
                     /locus_tag="PLD82_03015"
     CDS             complement(1441..2427)
                     /locus_tag="PLD82_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOG64393.1"
                     /translation="MSGQVPAVGFKMFMERHQDVVLQTHDYPDPDAIAAAYGLSVLIS
                     TLGGSAVICHTGAITRAITQEMISILQIPILPAVLAPGRENSIIVVDGRIRNDNVTKF
                     PGKYIGEIDHHPGEIDPDGDSWHDVRPHYGSTSSIVGEYWRDLGLPIPREVATALAIG
                     LNTDTQRLLRGVTGEDIEIYAWLFRHADREYLQYVLNNNIERADFAWFSKAIANLEVQ
                     DRFGMTDLGEMDNPALLAIICDFLLTAREINVMLTLAQSGGRVLLSMRSESPSHAADR
                     IVQQITTGIGSGGGHRSMAAGSCQFSDNSPPEFVKRLIRERFFGAARFTAHP"
     gene            2580..3050
                     /locus_tag="PLD82_03020"
     CDS             2580..3050
                     /locus_tag="PLD82_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64394.1"
                     /translation="MEDLQKRFLEALQLAASGKSTQAEKAFTAVFDSDPQGNLADDAL
                     CNIAILALNQNAIAQAESIFTRLLQDYPTATIAEYQGCSEHGRTGAKALLGLVNCSLA
                     RGNAALAAEQIAKLKDYSDSWVDTASGKKTYGELAAELLKTWEQANKEYQEQMK"
     gene            3050..3538
                     /locus_tag="PLD82_03025"
     CDS             3050..3538
                     /locus_tag="PLD82_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64395.1"
                     /translation="MDTRFTEFGMWRLPERVEKLESTPENRETLSLEAAFCPKGHNLL
                     DRDGNLAGGPGIVLSFRRPNGETGKVLISPRLEDLAKKVIEGDLVRGERVSLFCPVCG
                     EPLPVLAQCDRCHTGDMCVLYCSSALSIDDSVAFCNVVGCPNAMLIRSDRVIRALERD
                     ML"
     gene            3684..3754
                     /locus_tag="PLD82_03030"
     tRNA            3684..3754
                     /locus_tag="PLD82_03030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3716..3718,aa:Gly,seq:ccc)
     gene            complement(3831..4631)
                     /gene="lgt"
                     /locus_tag="PLD82_03035"
     CDS             complement(3831..4631)
                     /gene="lgt"
                     /locus_tag="PLD82_03035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG64396.1"
                     /translation="MHPILFRFVHPLDGRLIEIHSYGVLILAGFVAGYLLLRWNARQR
                     TLTIDKREILWLTGIVLASSMAGAKIFDVLVMLPDIIASGRSLLEAFTGGGFMFYGGV
                     AGALISFVWYCRRQHLDVRVWLDLFAPALALGQVFGRMGCFMAGCCYGAPTTCSLGVV
                     FDHPDNTVTPHGVALHPTQLYESGFALLLFVVLEFLWFKRRPRIPGGLMAVWALGYAI
                     FRFYVEFVRFDPRGSTLFGVLTPSQVVSLVLVSGAAIWLGSHMGRGRT"
     gene            complement(4832..5197)
                     /locus_tag="PLD82_03040"
     CDS             complement(4832..5197)
                     /locus_tag="PLD82_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOG64397.1"
                     /translation="MGVASLVLGIISIVFCFPPASIIGIITGIIAIVLGVMAKKNEEE
                     KGKATAGFVMGIIGLSLSALLWISCAYACNRVAYEMEKHGITSIEQGFKELEKVAKDP
                     KTHKALDQIRKMGESLTNE"
     gene            5386..5865
                     /locus_tag="PLD82_03045"
     CDS             5386..5865
                     /locus_tag="PLD82_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64398.1"
                     /translation="MANRPGGLTALAVLNFVFAGLGILGVLALAALMTAGSAIVAGAQ
                     KAADAMAEQGGEAAVAAAAASSAMGQVSAIGWLVVILSIINIVLLIISGVGFLKQSKG
                     KGQKVAMIYGIFGIATTILNLVIFSTGFGFSAIIGLIYPVLLLVLPMTVFKDAFVNP"
     gene            6209..6349
                     /locus_tag="PLD82_03050"
     CDS             6209..6349
                     /locus_tag="PLD82_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64399.1"
                     /translation="MKPILALSENPAYTKALERALTQFEDLYQILDEKQFKELVLNPG
                     FS"
     gene            6413..>6698
                     /locus_tag="PLD82_03055"
     CDS             6413..>6698
                     /locus_tag="PLD82_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64400.1"
                     /translation="MKASHETRAVPVIAVSSRSDEAHRYKLLAMDVHECFAFPFSRRE
                     FVLRCERAIEAWHEEKNWKDGVHLLRGYRQVAQKTDAEKREMQEILAAQEA"
BASE COUNT         1500 a         1818 c         1876 g         1504 t
ORIGIN      
        1 ccccgttttg ctgttggggc ggtggtactc caaggctggc cgccagtgcg gcgtacttgt
       61 catgaatctt tttcatgtat ccgccgccgc cggctttttt tgcgacgatg attccgacaa
      121 agatcatgat gaccgcaaag gcaacgccaa tgattggccc aagtatctgc atatccggca
      181 tattcaccgc ctcctggctc agttcgtgca acttgcatcc agtatatccc gtccggaggc
      241 attttcaatc cccgaaaggc cggacggcag ggtgattgca tgcgggggca ggcgcaaaat
      301 atttgcaata ttgttatcaa tggccgatga aaaccgggaa ttggagcgtg ttgacacagg
      361 gatgccttcg gggtcatact ccttgtgagg gaaacaagag cgcaaggggg gatacatgaa
      421 aggaatcatt cggtggggct gtcttgtctg cagcatcgtt cttacggctt ttaccctggc
      481 cggctacgat cagaaacagt acaacgaagt caaggctgga aacgagaaca aaacctacaa
      541 tttttttgat ctctcgggcg ccgatctctc aggcatgaat ctcgagatgg cttgtttccg
      601 tgaagccaat ctgatcaggg ccaatctcgc aaaggccaac ctcagctacg ccaatctcaa
      661 aggcgccttt ctcaacggtg caaatcttaa agatgccaat ctggcgtatg cggatatgcg
      721 gggcgccaat tttttggaag ccgacttgag cggcgccatc atcgccggag cacaagtccg
      781 cggggcacga tttttggccg ccaagctctc cgaagcccaa aaaaaataca tcgaggagaa
      841 cgggggcgag ttttaaccgg ttccccggtc ccgtattcct cgcatcgttt cgattcggct
      901 gtttcagccg acacgccggt tagcggctgt actgctccac tcgtgtgctt ttcctcatgt
      961 caagcaggcg cagcaggtgt tttttttcca tggtgatcag tatctggagt tctgtgtgac
     1021 attcctcgcc ggggcagagc gtccagatct cggtaaacag gaggagggca tctttttcga
     1081 actggatggc aagatcgagc gcagcgtccc ggtcagcgag ccttgtttga ctggcatgct
     1141 ccctgaagta ttccatcgaa agaagaccgt ccagtgtttc tttatttgcc tcaggtgttc
     1201 cggttccatg ccggtctatg caggtcttga ttccggccag gatttcacgt tcttcctcga
     1261 caaggtcggt gattgagagg gcgacatccg gatcggcatg gttgcccagt gcaagaaaaa
     1321 cgtcaagcgc gtgtttcttg aactccagca atgtcctcaa ggcgccagat agtttcatga
     1381 tatcccctcc ggtgggccgg aactggtcct gctttgagta tactcccggt cagggatttg
     1441 tcatgggtgg gcggtaaaac gggcagcacc gaaaaaccgt tcacggatca ggcgtttgac
     1501 aaattcgggc ggactgttgt cgctgaattg gcaggatccg gcggccatgc tccggtgtcc
     1561 ccctccgctc ccgatcccgg tggtgatctg ttgcacaatc cggtcggccg catgggatgg
     1621 gctttcactg cgcatcgaga ggaggacgcg tcctcctgac tgggcaagtg tcaacatgac
     1681 gttgatctcg cgagcggtca gcagaaaatc acagatgatg gccaaaagtg ccgggttgtc
     1741 catctcgccc aggtcggtca ttccgaaacg gtcctgcact tcgagattgg cgatggcctt
     1801 gctgaaccag gcgaagtcgg cccgctcgat attgttgttg aggacgtatt ggaggtattc
     1861 acggtcggca tgccggaaga gccaggcata gatctcgatg tcttcgcccg tgaccccccg
     1921 caggagccgc tgggtatccg tgttcaaccc gatggcgagt gctgttgcca cctcacgggg
     1981 gatgggaagt ccaagatcgc gccagtattc tccgactatg gaagaggttg acccgtaatg
     2041 ggggcggacg tcgtgccagg aatcaccgtc agggtcgatt tcgcccggat gatgatcgat
     2101 ttcaccgatg tacttgccgg gaaatttggt cacattgtcg ttccggatcc ggccatcgac
     2161 gacaatgatc gagttttccc ggcccggggc aaggaccgcc ggcaggatgg gaatctggag
     2221 gatgctgatc atttcctggg taatggcccg ggtgatcgcg cccgtatgac aaatcacggc
     2281 agacccgccc agcgtgctga tcaggacgga caagccgtag gccgccgcga tggcgtccgg
     2341 atcgggatag tcgtgcgtct ggagcacaac atcctggtga cgttccataa acatcttgaa
     2401 accgacagca ggcacttggc cggacatgga tacactccga tcccgtttga tctgcctaaa
     2461 tctagccaaa tttcggcgta caagccttgc gaagtcctcg tcattccggg attgctcccg
     2521 gagtcttccc ggggtagaat ggtgtatatg cactacgttc aaggagggat tgcacaatta
     2581 tggaagacct gcaaaaacgt ttcctcgaag ctctgcagtt ggccgcttcg ggaaaatcca
     2641 cccaagccga aaaagctttt accgctgtct ttgacagtga tccccagggg aatctggccg
     2701 atgatgcatt atgcaatatc gcgatcctgg cgctcaatca aaacgccatc gcccaggccg
     2761 agtccatctt tacccgcctg ctccaggatt atcccacagc caccatcgct gagtatcagg
     2821 gatgcagcga acacggacgg accggggcca aggccctgct tggtctggtc aactgctcgc
     2881 ttgcccgggg aaatgccgcg ctggccgccg agcagattgc caagctcaag gactacagcg
     2941 actcctgggt ggacacagcc tcagggaaaa aaacctatgg cgaactggct gccgagctcc
     3001 tcaagacctg ggagcaggcc aacaaggaat atcaggagca gatgaaataa tggatacacg
     3061 atttacggag ttcgggatgt ggcgccttcc ggagcgcgtc gagaaactcg aatcgacacc
     3121 ggaaaaccgg gagacactgt ctctggaggc ggccttttgt cccaagggac acaacctgct
     3181 tgacagggat ggaaacctcg ccggcgggcc cgggatcgtg ctctcgttca ggcgtcccaa
     3241 tggagagacg ggaaaggttc tcatctcgcc gagactcgag gatctggcta aaaaagtcat
     3301 cgagggcgac ttggtgcggg gggagcgtgt cagcctcttc tgcccggtct gtggtgaacc
     3361 actccccgtt ttggcacagt gtgaccgctg tcataccggc gacatgtgcg tgctctattg
     3421 tagcagcgcg ctctccatcg atgattctgt ggccttttgc aatgttgtcg gctgtcccaa
     3481 cgccatgctg atcagatccg accgggtcat ccgggcgctc gagcgcgaca tgttgtaagg
     3541 attttttggt gaacaggatg ctgcccctgg cagggaaggg ttgagcggga tagggggcag
     3601 cgtcctgaac ggtttttgta aaacacaccg gagagttctt gacgcccatt tcccgtctcc
     3661 gtatattgta ggggtcaacc attgcgggtg taattcaatg gtagaatgtg agcttcccaa
     3721 gcttaaaacg tgggttcgat tcccatcacc cgctcagcga ccggccttta tagccggttt
     3781 ttttatattc ccgacaccat ggtgacagga accgggcccg aactccggtg tcaggtgcga
     3841 ccacgtccca tgtgcgaacc gagccagatc gccgcccctg agacaagcac cagactgaca
     3901 acctgagaag gcgtcagcac cccaaaaagc gtgctgcctc gcggatcaaa gcggacaaat
     3961 tccacataga acctgaaaat ggcatagccc agggcccaga ccgccatcag acctcccgga
     4021 atacgcggcc ggcgtttgaa ccagaggaac tccagcacga caaagagcag gagagcaaag
     4081 ccggactcgt agagctgagt cggatgaagg gcaacaccat ggggtgtgac cgtgttgtcc
     4141 ggatggtcaa acacgacacc cagcgaacag gtcgtcggtg ccccatagca acagcctgcc
     4201 ataaagcatc ccatccgtcc aaaaacctga cccaaggcca aggccggcgc aaaaagatca
     4261 agccagaccc gtacgtcaag atgctgcctg cgacagtacc agacaaagct aatcagcgcc
     4321 ccagcaacac caccgtaaaa catgaaacca ccgccggtaa aggcctcgag cagacttcgc
     4381 ccggaggcaa tgatatcagg aagcatgacc aacacatcaa agatcttggc cccggccatg
     4441 ctggaggcca gcacgatccc ggtcagccac aagatttcac gcttgtcgat cgtcagtgtc
     4501 cgttgacggg cattccaacg caggaggaga tagccggcca caaatccggc caggatcagc
     4561 acaccatagc tgtggatctc aataaggcgc ccatccagag gatgcacaaa gcgaaacaaa
     4621 atggggtgca tggggactcc tgaatatgtg agctgtacaa gacatccagt atgtgatgct
     4681 tcggtagccg attggaaggt tcggggtgct tttgcagaga gatctcgggg atcttactga
     4741 atttttgttc ataaaaaaaa gcagactgcc cgttgttggc agtctgctat cgatacggca
     4801 gtataaaacc gggctgcctg actgaacaag atcattcgtt ggtcaacgat tcgcccatct
     4861 tcctgatttg gtcaagagct ttgtgcgttt ttggatcttt ggccactttt tcgagctcct
     4921 tgaagccctg ttcgattgaa gtgattccgt gcttctccat ttcgtaggcg acccggttac
     4981 aggcgtacgc gcaggaaatc cagagcaacg cagaaaggga aagtccgata atgcccatga
     5041 caaatccagc tgtggctttc cctttttctt cttcgttctt tttggccatc acaccgagga
     5101 cgatggctat tataccggtt atgattccga tgatgctggc cggcggaaaa cagaaaacaa
     5161 tggaaataat accgagtaca agggatgcaa cacccatggg ggccctcctg aattcatttc
     5221 ataccaagac aaggtacccg taaacagtgc accatgcaag ggtttctctt cattttgcca
     5281 ccaaggcggc gcgttttttg tggtattgat tttttccgtc agtatggtat cttgtagtcg
     5341 tgccggctgt gtcggcaaac ttacacaaaa aggggttttt acattatggc taacagacct
     5401 ggtggactta ctgctcttgc tgtactcaac ttcgtttttg ccggactcgg cattcttggc
     5461 gttcttgccc tcgcggctct tatgaccgct ggctcggcta ttgttgctgg cgcccagaag
     5521 gccgctgatg ccatggccga acagggtggc gaggctgctg tcgccgctgc cgctgcttcc
     5581 tcagccatgg gacaggtcag cgctattggc tggctcgttg tgatcctttc gatcatcaac
     5641 attgtcctcc tcatcatctc cggcgtcggc ttcctcaagc agtccaaggg caaaggccag
     5701 aaggttgcca tgatctatgg tatcttcggg attgccacca ccatcctcaa cctcgttatc
     5761 ttcagcaccg gtttcggttt ctcggccatc atcggcctga tctaccccgt cctcctcctc
     5821 gtccttccga tgactgtttt caaggatgcg ttcgtcaatc cgtaattcga gtgacagtat
     5881 ttattttcta cagaaaaagc cggccgagag ggccggcttt ttctgtcccc agggagtcct
     5941 ttgcgagcgt cccccgcctt tggatgttca tcaaaaaagc actgccaatg aatgatcact
     6001 attctggcgt tggtccggat gttgggcggg tgtcgtgtgg agtgtgttct caagcgtgac
     6061 acgaagtgcc agccgaaggt ccccttcgga gcaggcatcg gctcgggcgg accgaatgaa
     6121 gactcgggag cgttgtgcaa tcccggcttg cctccggaat ggacggcgtg gtatcatcgg
     6181 tcaagaccgg aacagcgcgg agcggagcat gaaaccaatt ctggcattgt cggagaatcc
     6241 agcctatacc aaggccctcg aacgggctct gacgcagttt gaagacctct accagattct
     6301 tgacgaaaaa caattcaaag aattggttct caatcccggg ttttcctaac aggccagcct
     6361 ggtcctcttt gatccggctt ccggtccggt cccttgggaa atcgtcagga agttgaaagc
     6421 atcgcacgaa acgcgcgccg ttccagtcat cgcggtgtcc tcaaggagtg acgaagcaca
     6481 ccgctataaa ctcctggcaa tggatgtgca tgagtgtttc gcctttccct tttcacggcg
     6541 tgaatttgtg ctccgctgcg agcgggctat cgaggcctgg cacgaggaga agaactggaa
     6601 ggacggcgtc catcttctgc gcgggtatcg gcaggtcgca caaaaaacgg atgctgaaaa
     6661 acgcgagatg caggagatcc tggcggcgca ggaggcgg
//
LOCUS       DAOSHE010000068         6685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_9974_length_6685_cov_4.376320, whole genome shotgun sequence.
ACCESSION   DAOSHE010000068 DAOSHE010000000
VERSION     DAOSHE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9974_length_6685_cov_4.376320"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLD82_03060"
     CDS             complement(<1..355)
                     /locus_tag="PLD82_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOG64401.1"
                     /translation="MPILPTSDEMRRIDTVASTESGIPAAVLMENAGQAAAACISSHN
                     PGASIACLCGTGNNGGDGYVIARHLVRMGHTVRVYSVGDLSHQSELCRFQHEYFSLYS
                     GLAVDAWQPGMDLGKS"
     gene            complement(493..1572)
                     /locus_tag="PLD82_03065"
     CDS             complement(493..1572)
                     /locus_tag="PLD82_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64402.1"
                     /translation="MIPRFAALCSLVLLMVSCSSESTVLTPTFRFFIPFGEGRGALGT
                     ASVTNDRMPLIGSFVVNDDRLGMFDRETGRLLVYSTDGKLLIDRILEKLESKKAWPAP
                     VLALSSSSVWIAKPEGAAEKRVYRVYTVPDDTTEMTTAVVFPATPAAILGKNAEAVEH
                     IILERLIPLDNESLIAVWHGLTSRGSGEVVSAMAISIADLKSRTVRTHLVDIDGVKKH
                     ESGDARFDSIVSVHQYKDTRQFILETQYRKTDLRSPFEKVLFIQDIEKQTLTKVELPW
                     SAWNALFGVGRDGGLFFIEDVLIHNAATRAIISVYRMDSSRETRYAIEADPARPSLGN
                     FVFSLEGSLYGYEAFDRGVNIYSWR"
     gene            complement(1604..2656)
                     /locus_tag="PLD82_03070"
     CDS             complement(1604..2656)
                     /locus_tag="PLD82_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64403.1"
                     /translation="MRIKTGFVLLTLLILFAFPLPSAQKLRVAVMDIANPDPDSATLQ
                     RFSSRVGQTIMRSLGALGEYEIISASDVEAARLDLASEYRNLENAMMRALGRRLKADI
                     VINGTVTAPNLSMLGVEIRILDVGKSQFLDTITVSNRREMEDIIVGEVVNRVATDTGV
                     KFLGRGSNLVETTTTVTGSPQSSSSSSSSSSVGTTSSQSNSTTRSDEATRVAQRDISL
                     NAIFHWGNVLPLFGSESKTLLGIDFVFDVERTYYSFGFGFTMAGAETIDIGVFFETHF
                     FENDYSRNWQVYLRTGPALAYPLQSDVESGFKLNTGLGVRFAIPYFVFDAGFGIDYYS
                     SQSSSLYIRAGGGFHF"
     gene            complement(2728..3492)
                     /gene="hisF"
                     /locus_tag="PLD82_03075"
     CDS             complement(2728..3492)
                     /gene="hisF"
                     /locus_tag="PLD82_03075"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOG64404.1"
                     /translation="MHAKRIIPCLDVNAGRVVKGIQFVDLIDAGDPVENAIRYDQEGA
                     DELVFLDITASHEQRDTMIDVVRRVAESVYIPFTVGGGIRTVDDMRRMLLAGADKISV
                     NSAAVKNPGIISEGARFFGSQCIVLAIDARKRADGGWEVYTHGGRNPAGLDVIDWALR
                     GEESGCGEILLTSMDRDGTKSGYDIDLLKAVSQSVRVPVIASGGAGTLDHFSSALLEG
                     GADAILAASLFHFREIGISQTKRHLRDAGISVRLCE"
     gene            complement(3482..4213)
                     /gene="hisA"
                     /locus_tag="PLD82_03080"
                     /pseudo
     CDS             complement(3482..4213)
                     /gene="hisA"
                     /locus_tag="PLD82_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
     gene            complement(4204..4812)
                     /gene="hisH"
                     /locus_tag="PLD82_03085"
     CDS             complement(4204..4812)
                     /gene="hisH"
                     /locus_tag="PLD82_03085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG64405.1"
                     /translation="MIAIVDYGMGNLRSVAKALERFGYPCTVTADPHCIAHAKGVILP
                     GVGAFGEAAFNLRQSGLVDEVMRAAKDGRPFLGICLGYQLLFEESEEAPGVPGLGIFP
                     GRVKRFAHGLKIPHMGWNSVEFNGKHPITTGQKQPAWFYFVHSYYVEPADQSLVVGRS
                     EYGIPFAAAIASGNLFACQFHPEKSQSAGLSLIKRFGELSCV"
     gene            complement(4809..5393)
                     /gene="hisB"
                     /locus_tag="PLD82_03090"
     CDS             complement(4809..5393)
                     /gene="hisB"
                     /locus_tag="PLD82_03090"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOG64406.1"
                     /translation="MRTASIERSTKETRIRLELALDRPEPVDIKVPGGFFRHMLELFA
                     FHGGFSLACDVTGDTDVDMHHTVEDTAICLGQAFKIALGDKKGIARYGLFSVPMDEAR
                     AVVHLDISGRPFVRMDGKELVGTCGSFDAELVAEFLRAFAFNAGITLHVSVDCGENLH
                     HMIEAIFKALARALRIAIDQDGRGILPSTKGVID"
     gene            complement(5399..>6685)
                     /gene="hisD"
                     /locus_tag="PLD82_03095"
     CDS             complement(5399..>6685)
                     /gene="hisD"
                     /locus_tag="PLD82_03095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG64407.1"
                     /translation="ELRVVEYGEARTLLRQRLLHISRDQNTIETVAAILGDVAKRGDA
                     AVLEYTSRFDGVTLDQAGMQVRPSEIEASVKRIEKTELGQALRHAAARIRNFHERQLE
                     KSWIMHEDGAILGQVVHPLSSAGLYVPGGKAPYPSTVLMNAIPATVAGVRRLALCTPA
                     NHCSDAVLYAASIAGVTEIWKVGGAQAIAALALGTESIQPVDKITGPGNRYVAEAKRQ
                     VFGLVDIDMVAGPSEILVLADETSDPDLVVRDLFSQAEHDQDAGSLLVSTSQDLVARV
                     NERIEECVGGESRAAIITESIRKNGMAILVPDLDSAWEIINLYAPEHLEILATVPLAT
                     VLSSVRNAGAIFYGNFAPEPLGDYIAGPNHTLPTAGTARYASPLGVYDFQKRTSLIAP
                     TRELYDKLVDDAARIAHAEGFMAHEASVLARKKRKD"
BASE COUNT         1581 a         1855 c         1812 g         1437 t
ORIGIN      
        1 cgcttttgcc aagatccatc ccgggctgcc atgcatcgac cgcaagaccg gaatagagtg
       61 aaaaatactc gtgttgaaac cggcagagct cgctctggtg tgagagatcg ccgaccgaat
      121 agacccggac agtgtgtccc atacggacaa gatggcgcgc gatcacatag ccgtccccac
      181 cattgtttcc cgtgccgcag agacaggcga tactcgcccc tggattgtgt gaagagatgc
      241 aagccgctgc agcctgtcct gcgttttcca tgagaactgc agcgggaatc ccggactccg
      301 tactggcaac ggtatcgatc cggcgcattt cgtctgaagt cgggagaatc ggcatacgga
      361 ttcaagcctg ccctgtagtt ggtcttctcc catggtacga cagcatgtca caaaacacaa
      421 gcccgggtcg cgacacgtgc atcccgatat ctctgattac gggagagaaa aacagattca
      481 gtcacgatac tctcatctcc aggaatagat gttgaccccg cgatcaaagg cttcatagcc
      541 atagagcgat ccctccaagg agaaaacaaa attcccgaga ctggggcgtg ccggatcagc
      601 ttcgatggcg taccgtgttt cccgcgagga atccatccgg tagaccgaga taatggcacg
      661 ggtcgcggca ttatggatca aaacatcctc gatgaaaaaa agtcctccgt cacgcccgac
      721 accgaagagt gcattccatg cggaccaggg aagttcgacc ttggtcagag tctgcttttc
      781 aatatcctgg ataaagagga ctttctcgaa aggactgcga agatccgttt tacgatattg
      841 ggtttcaagg atgaactgcc tggtgtcctt gtactgatgc acggaaacaa tggaatcaaa
      901 acgggcatcc ccactctcat gcttcttgac accatcgatg tcgacgaggt gtgtccggac
      961 ggtgcgcgat ttgaggtcgg caatgctgat ggccatggcc gaaacaacct caccgcttcc
     1021 acgcgatgtc aatccatgcc agaccgcaat caaggattca ttgtccaagg ggatcaatcg
     1081 ttcgagaatg atgtgttcga cggcttcggc atttttgccg aggatcgcgg ccggtgttgc
     1141 cggaaagaca acggcggttg tcatctcggt tgtatcgtcc ggaacagtat atactcgata
     1201 gactcttttt tcggcagcac cttcgggttt ggcgatccag actgacgaag acgacaaggc
     1261 aaggaccgga gccggccagg cctttttgga ttcaagtttt tcgaggatgc ggtcgatcag
     1321 aagttttccg tctgtcgaat agaccaggag tctcccggtt tcgcggtcaa acatcccgag
     1381 acgatcgtcg ttgacgacaa aagacccgat caggggcatc cggtcattgg taacggaagc
     1441 agtaccgaga gcgccccggc cttcaccaaa ggggatgaag aatctgaatg tcggcgtcaa
     1501 tacggtactc tcactggaac aggagaccat caacagaaca agagaacaca gcgctgcgaa
     1561 acgaggaatc atggcgacct tcatggacgt gccggagggt acgtcagaaa tggaatcccc
     1621 cgccggctct gatgtagagt gaactgcttt gactcgaata ataatcaatc ccgaaaccgg
     1681 catcaaacac aaaatagggg atggcaaagc ggacaccaag accggtattc agtttgaacc
     1741 cgctttcgac atcactttgc aggggatagg ccagagccgg cccagtcctg agatagacct
     1801 gccagttccg ggagtagtca ttctcgaaga agtgtgtttc aaaaaagact ccgatatcga
     1861 ttgtctcggc acccgccatg gtaaaaccga acccgaacga ataatacgtt cgctcgacat
     1921 caaagacaaa atcgataccc aaaagtgttt ttgactcgct tccgaagaga ggaagaacat
     1981 ttccccagtg aaaaatcgca ttcaagctga tatcacgttg tgccacccgc gttgcttcat
     2041 cactccgggt cgtgctgttt gactggcttg aggttgttcc cactgaagaa cttgaagagc
     2101 tggacgaact tgactggggc gagccggtca ctgttgtcgt ggtctcgaca agattggagc
     2161 ctcttcccaa aaacttgaca ccggtatctg tagcgacccg gttgacgacc tctcccacga
     2221 tgatatcttc catttcgcgg cggtttgaaa cagtgattgt atcgaggaac tggctttttc
     2281 cgacatcgag aatccggatc tccactccga gcatggaaag attgggggct gtgaccgtcc
     2341 cgttgatgac gatatcagcc ttgagccggc gcccaagggc tcgcatcatg gcgttctcga
     2401 gatttctgta ctctgaagca agatccagac gggccgcttc aacatcgctg gcgctgatga
     2461 tctcgtactc tccgagcgca cccaggctgc gcatgatggt ctgtccgaca cgggacgaaa
     2521 agcgctggag tgtggccgaa tcgggatccg gattggcaat atccatgacg gccacccgca
     2581 acttttgggc tgaaggaaga ggaaacgcga agagaatgag cagggtaagc agtacgaaac
     2641 cggtcttgat cctcatgttc ctgactccat cagcgattga tacatgcaca agccaaagta
     2701 tagcacattt ttccatgaag cgcagtatca ctcgcacaga cggacagaaa taccggcatc
     2761 ccggagatgg cgcttggtct gactgattcc gatctcgcgg aaatggaaga gagaggctgc
     2821 caagatggca tctgccccgc cctcgagtag cgcggaactg aaatggtcca atgtccccgc
     2881 cccacccgat gcaatcaccg ggacccggac actctgtgaa acagccttga ggagatcgat
     2941 atcgtatccg gattttgttc catcacgatc catgctcgtc aggaggatct cgccgcatcc
     3001 ggattcttcg ccacggaggg cccagtcgat gacatccagt ccggcagggt tgcggccgcc
     3061 atgcgtgtac acttcccagc ctccatcggc ccgcttcctg gcatcgattg caagaacgat
     3121 gcattgtgat ccgaaaaacc gtgccccttc ggagatgatc ccggggtttt tgacggcagc
     3181 ggaattgacc gagatcttgt cagctccggc gagaagcatc cttcgcatgt catcgactgt
     3241 cctgatcccg ccaccaacgg tgaagggaat atagaccgac tcggctaccc gccggacaac
     3301 atcgatcatc gtgtcgcgtt gttcatggga ggccgtgata tcgagaaaca ccagctcatc
     3361 ggcaccctcc tgatcataac ggatcgcgtt ttccaccggg tctccggcat caatcagatc
     3421 gacgaactgg atgcccttga cgacccggcc cgcattgaca tcgaggcagg gaatgatgcg
     3481 tttagcgtgc atcctgtacc gcctgaatgg cctcggtcag agtgaatcgt ccttcgtaca
     3541 gagctgtgcc gacaatcact ccgtccagat tgcgtattcc ggcttcggcc agtctgtgca
     3601 ggtcctcaag gttggagatg ccgccggaag cgatcaacgg acaggagatg acatcaccca
     3661 tctttcgctg ggccggaata ttcggcccct tgagggtgcc atcggtgtcc acatccgtat
     3721 agatcacgcc agccagtggg agattattga atgatgccgc aaagctttag acattgtccg
     3781 ccgagacact ctgccaacct tcgacagcca gtttgtcatt tttggcatcc gctccgagaa
     3841 tgacccgacc cgggtagaga ttggccagac tttccaccag gagtttgttg cgctgtgctc
     3901 cggtcccgat cacgacccga tcgattccct gctcaaacag ggcgcgggca tcttcgagag
     3961 aacggacccc tcccccgacc tcgatcatgc aggagacagc catacgtatg gcctggatcg
     4021 attccatgtt tctgcggata ccgtctcggg ctccatccag atcgacaaca tggatcagtt
     4081 ctgctccacc ctcttcccac gccttcgccc gcgaggccgg gtcatcatcg tagacttttt
     4141 ccatcgcata atcgcctttg aaaagacgga cacaacggcc gccacgaaga tcaatcgcag
     4201 ggattatacg catgagagtt ctccaaatcg tttgatgagt gaaagcccgg ccgattggct
     4261 tttttcggga tggaactgac aggcaaagag attgccgctg gctatggccg cggcgaaggg
     4321 aattccgtat tcgctccgtc ctacgaccag actctgatca gcaggttcga cgtaatagga
     4381 atggacaaaa taaaaccagg cgggttgttt ttgcccggtt gtgatcggat gttttccatt
     4441 gaactcgacg gaattccatc ccatatgggg aatcttgaga ccatgggcaa aacgtttgac
     4501 acgtccgggg aagatgccca aaccggggac cccgggggcc tcttcactct cttcaaacag
     4561 cagctggtag cccagacaga taccgaggaa aggtcttcca tcctttgcgg cccgcatgac
     4621 ttcgtccaca agaccgcttt gccggaggtt gaaggctgct tcaccgaagg cgcccactcc
     4681 gggaaggatg acaccctttg cgtgtgcgat gcagtgcgga tccgctgtca ccgtacaggg
     4741 atatccaaag cgctcaagag ctttggccac actccgcaga ttccccatcc cgtaatcgac
     4801 aatcgcgatc aatcaatcac tcccttcgtg gaaggaagga taccccggcc gtcctgatcg
     4861 atggcgatcc gcaacgcacg cgccaaagcc ttgaaaatgg cctcgatcat gtgatggagg
     4921 ttctcgccac aatccaccga gacatggaga gtgatcccgg cgttgaaggc aaaggctctc
     4981 aggaattcgg cgacaagttc ggcatcaaaa ctcccgcagg ttccgaccag ttccttgccg
     5041 tccatccgga caaagggacg tcccgagata tcgagatgga caaccgcccg ggcttcgtcc
     5101 atgggaacgg aaaacagccc gtaccgggca atcccttttt tgtcaccgag cgcgattttg
     5161 aaagcctgcc ccagacagat ggctgtatcc tcgacagtgt gatgcatatc gacatccgtg
     5221 tcgccggtga cgtcacaggc gagggaaaac ccaccatgga aggcgaaaag ttcgagcata
     5281 tgcctgaaaa agcctcctgg aaccttgatg tcgacaggct cgggtctgtc cagtgcgagt
     5341 tcaagtctga tcctggtctc tttggtggaa cgttcaatac tggcagtccg cattacactc
     5401 aatccttacg tttttttctc gcaaggacgc tggcctcgtg tgccatgaat ccttcggcat
     5461 gggcgatcct tgccgcatca tcaacgagct tgtcatagag ttcccgggtc ggtgcgatca
     5521 gggaggttct tttctggaag tcatagacgc caagaggaga cgcatagcgg gctgtgccgg
     5581 cagtcgggag ggtgtgattg ggcccggcga tataatctcc gagaggttca ggtgcaaaat
     5641 ttccatagaa aatagccccg gcgttccgga cagaagagag aacagtggcc aatggtacag
     5701 ttgcgagaat ctcgagatgc tcgggagcat agagattgat gatctcccat gcggaatcga
     5761 gatccggaac aagaatggcc attccgtttt tccgtatcga ttcggtgatg atggcagccc
     5821 tgctctcacc gccgacgcat tcctctatcc gctcatttac gcgagcaaca aggtcttggg
     5881 aggtgcttac gagaagagac ccagcatcct gatcatgctc ggcctgggag aagagatcgc
     5941 gtacaaccag atcgggatca ctggtttcgt cagccaggac cagaatctct gatggaccag
     6001 cgaccatatc gatgtcaacg agaccgaaca cctgccgctt ggcttcagca acataacggt
     6061 ttccgggacc ggtgatcttg tccaccggct ggatggattc cgttcccagt gccagtgcgg
     6121 ctattgcttg cgcaccgcca accttccaga tttcagtcac accggcaatg ctggctgcat
     6181 acagaacggc atcggaacaa tgattggccg gggtacagag ggcaagccgt cgtactccgg
     6241 cgacggtcgc cgggatggcg ttcatcaaaa cggttgacgg atatggagct ttcccgccgg
     6301 ggacatagag accggcagag gatagaggat ggacaacctg tccgaggatt gctccgtcct
     6361 cgtgcatgat ccagcttttt tcaagctggc gttcgtggaa attccggatc cgggctgcag
     6421 catgtcgaag agcttgtccc agttccgtct tttcaatccg cttgactgaa gcttcgattt
     6481 cggagggacg aacctgcatc ccggcttggt caagggtaac accatcaaaa cggcttgtat
     6541 attcgaggac cgcagcatct ccgcgcttgg cgacgtcgcc aaggatggcg gcaaccgtct
     6601 cgattgtatt ctggtccctt gaaatatgca ggaggcgctg ccgcaaaagc gtccgcgcct
     6661 ccccgtattc gacgacccgt aattc
//
LOCUS       DAOSHE010000069         6651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10045_length_6651_cov_3.494694, whole genome shotgun sequence.
ACCESSION   DAOSHE010000069 DAOSHE010000000
VERSION     DAOSHE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_6651_cov_3.494694"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="dapB"
                     /locus_tag="PLD82_03100"
     CDS             <1..796
                     /gene="dapB"
                     /locus_tag="PLD82_03100"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG64408.1"
                     /translation="KVGVVGAGGRMGKNLINAIFENPQTVLAAAVDAPQFPGLGTDAA
                     LNAGRPASGILIGSDLAAAFAASDVLIDFSFRDAFPGVLAAARTAKKPLVVGTTGLEA
                     AHFAMLDEAAREIPVFHANNYSIGVAVLKSLVEQAAHRLGPAFDIEVTEVHHKLKKDA
                     PSGTAITLAEAAAAGRGLVPDECFCYGREGMVGERPQDQIAIHAIRGGDVVGEHTVYF
                     FGDAERVTLGHVATSRMNFAHGAVRAAAWVVTKPAGRYGIDHLLVE"
     gene            793..1263
                     /gene="folK"
                     /locus_tag="PLD82_03105"
     CDS             793..1263
                     /gene="folK"
                     /locus_tag="PLD82_03105"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG64409.1"
                     /translation="MMHTYLLSLGANLGRRLITLRLAVRELARRGEILAVSSVWETAP
                     LGMKGEHPAFLNCAVHFDTYLDPRQLLACCKRLEERAGRDLEERYQSRPLDIDIITWS
                     GGEWQDPSLVIPHPRAGKRRFVVIPCNEIIPDTFQEPEEPQDMAFFCGGDALLP"
     gene            1291..2079
                     /gene="panB"
                     /locus_tag="PLD82_03110"
     CDS             1291..2079
                     /gene="panB"
                     /locus_tag="PLD82_03110"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOG64410.1"
                     /translation="MTTTRSIIRKKGHEPIVMVTAWDSITARIADDAGVDMILVGDSL
                     ANTALGYPNTLPVSLDAMYHHASAVSRTSGDALVVFDLPFLGCCGNMAEDVRACGRAL
                     KDCGCHAVKMEGTPLDLVRALTGQGIPVMGHLGLMPQRVHQYGGMFVQGRGNDAGTVL
                     ESARALVDAGIFSLVLECVPEDLGARVAREIPVPVIGIGAGRFVDGQVQVLADLLGLL
                     PGKTPKHARRYADLLDQATKAVSEYASDVRASRFPGEEQTFGDA"
     gene            2079..2927
                     /gene="panC"
                     /locus_tag="PLD82_03115"
     CDS             2079..2927
                     /gene="panC"
                     /locus_tag="PLD82_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOG64411.1"
                     /translation="MLVIHDKSAIRKLVASWQAQGQRVGLVPTMGALHAGHISLVRQS
                     RESCDRTVVTIFVNPTQFGPSEDLALYPRALQADCAMLEDEGVDAVFAPPEGSMYAAD
                     HSTWVTVDGLTSGLCGASRPTHFRGVTTIVSKLFHIIPADEAFFGQKDYQQATVIRRM
                     VRDLDLDLRVTVCPIVREPDGLAMSSRNRFLTAEQRQAALVLSRALGDVSQMVGEGAT
                     PDDCRKFLEHAIGSEPLAELDYAAIVDADTLGEIRDFSRPAVAALAVRFGATRLIDNI
                     LLPPSG"
     gene            3030..3878
                     /locus_tag="PLD82_03120"
     CDS             3030..3878
                     /locus_tag="PLD82_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOG64412.1"
                     /translation="MAEIQQQESREAVYTGYQVKTPLFEGPLDLLLYLVRNYEINIYD
                     IPVTLITEQFLGYIKLLERLDLEVASEFIVMAAHLILIKSRMLVPGDPEHEDDEEDPR
                     TELVAQLIEYQKFKEAAHILEESESLSRAVLERKAGQIVFDFPGDESNWVDVKLFDLV
                     NAFSRIMSRSEKEPPSYPVFDDVEDNYDPEEKITQIRTMLEIHSRIEFNEIITPATRR
                     GEIIIIFLALLHMIKRGYIVVRQHRLFGDITIFARNAAPDGVASGIVEEPEEDTGSQQ
                     GSGDGV"
     gene            3875..4429
                     /gene="scpB"
                     /locus_tag="PLD82_03125"
     CDS             3875..4429
                     /gene="scpB"
                     /locus_tag="PLD82_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG64413.1"
                     /translation="MSREEKKALVEALLFIENEPVEYGKLVKHTGLEQQELSGLVEEL
                     KTEYELRQSGIQIVEVAGGCQMIAHPRFGLVLSQIYGVKNNNKLSQTALEVLAIIAYK
                     QPITKTEIEEIRGVSCERVIKLLMEKDLVVISGRRETPGKPLEFGTTKNFLKVFGLKS
                     INDLPKLREIKEMEFEIRENGDNE"
     gene            4422..5294
                     /locus_tag="PLD82_03130"
     CDS             4422..5294
                     /locus_tag="PLD82_03130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG64414.1"
                     /translation="MNEPMEGARLDIDVLLQDADTLPAFPSIVTEILARIDDPLSVPD
                     DFTEIISRDQVMVSKVLKFANSAFYGFPRTIVTLKEAVIILGLDTLKSIVLAISSYST
                     LNANNKGYGLDKGGLYQHSICCAMIARILAKKYKSENKEKFFIAALLHDVGKLLLDPW
                     LSRFKTEMIDGIRKGNLAPWEAERRVLGFDHAFVGSKLIEGWNLPEVLIHVTRYHHTP
                     EKAPLEHRAIVEMIQAANIISRRIRGGLGGEFIPPEGKAMDELQHVLPLAEDEVRSIV
                     REVRTYLLHINTFI"
     gene            5361..6455
                     /gene="pheA"
                     /locus_tag="PLD82_03135"
     CDS             5361..6455
                     /gene="pheA"
                     /locus_tag="PLD82_03135"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG64415.1"
                     /translation="MSDEQQTLGTLEGLRSKIDEVDRGIAALLEERMDLALQIGQVKA
                     REGLPVYHPEREHSVISRIVEHFHAGHFPLDSAKRIYTEIVGACRDAQRALRVAYLGP
                     HATYSHMALERRFGGSALAIPQETIADVFSAVISQNADCGVVPVENSNEGSVAHTLDL
                     LAEGRVHVIGEVLQPVHHCLLSTQTTMDRIRRVLAHPQALAQCRRWISRNLPHATVVE
                     TSSNGKAAEIAASEDGSAAIAARIAARLYRLNLLAESIEDNADNITRFFIIGAKPTMP
                     TGKDRTSLVIAVKDRPGILHDLLDVFARRGINMSRIESRPSRIKAWEYVFFVDVDGHA
                     QVSPLSDALSELDAHVRTLRVIGSYPRSED"
     gene            6458..>6651
                     /locus_tag="PLD82_03140"
     CDS             6458..>6651
                     /locus_tag="PLD82_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOG64416.1"
                     /translation="MNNHFVIAGLGLLGGSLSLALKRRDPECRIHVLGRDPERLETAR
                     REGFCDSWSVDSKNLPAGIRG"
BASE COUNT         1390 a         1958 c         1912 g         1391 t
ORIGIN      
        1 aaaggtcgga gtggtcggcg ccggcggacg gatgggaaaa aacctcatca acgcgatttt
       61 cgaaaatccc caaaccgtac tcgccgccgc tgtggatgcg ccgcagtttc ccggtctcgg
      121 gaccgatgca gcgctcaatg ccggaagacc ggccagcggt atcctgatcg gctccgatct
      181 ggctgccgct tttgctgcca gcgatgtctt gatcgatttc agcttccggg atgccttccc
      241 gggtgtcctg gccgcggcca ggacggccaa aaaacctctt gttgtcggaa caacaggcct
      301 cgaagccgcc cactttgcca tgctcgacga ggccgcccgg gagattccgg ttttccacgc
      361 caataattac agcatcggtg tggcggttct caaatcgctt gtggaacagg cggcccaccg
      421 tctcgggccg gccttcgata tcgaggtgac cgaggttcat cacaaactga aaaaggatgc
      481 tccatccgga acagcaatta cccttgccga ggcggcagcc gccggtcgtg gtctcgttcc
      541 cgacgagtgt ttctgctacg gtcgtgaggg aatggtgggc gaacgtccgc aggaccagat
      601 cgcgatccat gccatccggg gcggtgatgt cgtcggtgag cataccgtct atttctttgg
      661 tgacgccgaa cgtgtcaccc tggggcatgt tgcgacctca cgcatgaatt ttgcccatgg
      721 tgctgttcgt gcagcagcat gggtggtcac aaagccggcc ggacggtacg gaatcgacca
      781 tctcctggtg gaatgatgca tacctacctc ttgtccctcg gggccaatct cggaagaaga
      841 ctgatcacgt tgcgactggc ggtccgtgag cttgcccgcc gcggggagat cctggcggtc
      901 tcctcagtct gggaaacggc acccttgggg atgaaggggg aacaccccgc cttcctgaat
      961 tgcgctgttc attttgatac ctatctcgac ccccgtcagc tccttgcatg ctgcaagaga
     1021 cttgaagaac gggccgggcg tgacctcgag gaacgatacc agtcccgacc gcttgatatc
     1081 gacatcatca cctggtcggg cggggaatgg caggatccct ccctcgtgat tccgcatccg
     1141 agagcgggca agagacgttt tgtcgtcatt ccctgcaacg aaatcattcc agatacgttt
     1201 caagagcctg aagaaccgca ggacatggca ttcttctgcg gcggtgatgc ccttcttccc
     1261 tgagggcggg accaagagag gagcctgtca gtgacaacca cacgatccat catccggaaa
     1321 aagggccacg aacccatcgt catggtcaca gcctgggaca gcattacggc ccggattgca
     1381 gatgatgccg gagtggacat gatcctggtg ggagactccc tggccaatac ggccttggga
     1441 tatcccaata ccctcccggt cagtctggac gccatgtacc atcatgcttc ggcggtcagc
     1501 cggaccagcg gagatgccct ggtcgtcttt gatctgccct tcctcgggtg ttgcgggaac
     1561 atggccgaag acgtccgggc ctgtggccgg gccctcaaag actgcgggtg tcacgccgtc
     1621 aagatggagg ggacgccgct tgacctggtg cgggccctga ccggtcaggg gatcccggtc
     1681 atgggccacc tcgggttgat gccccagcgt gtccaccaat atggcgggat gtttgtccaa
     1741 gggcggggaa acgacgccgg gaccgtcctc gagtccgccc gggccttggt cgatgccgga
     1801 atcttttctc tcgtgctcga atgcgtcccc gaggacttgg gtgcccgcgt ggcccgggaa
     1861 atccccgttc cggtgatcgg aatcggtgca ggccgctttg tggacgggca ggtgcaggtt
     1921 ctggcggatc ttttgggcct tctcccggga aagacgccca aacatgcccg gcgctatgcc
     1981 gatcttttgg accaggcaac caaggccgtc tcggagtacg cgagcgatgt ccgcgccagc
     2041 cgttttcctg gtgaagaaca gaccttcgga gatgcctgat gcttgtgatc catgacaaga
     2101 gcgcgatccg gaaactggta gcctcctggc aggctcaggg acagcgggtg gggctggttc
     2161 ccaccatggg ggcgctccat gcggggcaca tctccctggt ccggcagtca cgggagtcct
     2221 gtgatcgcac cgtcgtgacg atctttgtca atccgaccca gttcggaccg agcgaggatc
     2281 tggccctcta tccccgggcc ctgcaggccg actgtgccat gctcgaggac gaaggggtgg
     2341 atgccgtctt tgcacccccc gaaggcagca tgtatgccgc ggatcactcg acatgggtca
     2401 cggtggatgg tttgacatcc ggactctgcg gggcctcccg cccgacccat tttcgtggag
     2461 tcacaaccat tgtcagcaag ctctttcaca tcatccccgc ggatgaggcc tttttcggcc
     2521 agaaggacta ccagcaagcc accgtcattc gccgtatggt ccgtgacctc gatctggatc
     2581 tccgggtgac tgtctgcccg attgtccgcg agccggacgg gctagccatg agttcgcgca
     2641 accggtttct taccgctgaa cagcgtcagg ccgccctggt cctctcgcgc gcactcgggg
     2701 atgtcagcca gatggtcggg gagggggcga ctccggatga ttgccgaaaa ttcctcgaac
     2761 acgcgatcgg ctccgaaccg ctggccgaac tggattatgc cgccatcgtg gatgctgaca
     2821 ccctcgggga gatccgggat ttcagtcgtc cggctgtcgc tgccctcgct gtccgcttcg
     2881 gcgcaacccg cctgatcgac aacatcctcc tgccaccctc cggttgacac gccgcccccg
     2941 accggtttga gtctgttcat caaaaaaaac gaacccgaac ggcagtccgc ctttacccgg
     3001 accggaaagt gtccgatact ggtagagata tggcagagat acaacaacag gaaagccgcg
     3061 aagcggtcta tacgggatac caggtcaaga cgcccctctt cgagggtccg cttgacctgc
     3121 tcctgtatct tgtacgtaat tatgaaatca acatttatga cattcctgtg acgctcatca
     3181 ccgagcagtt cctcggctac atcaagctcc tcgaacgtct ggatctcgag gtggcgtccg
     3241 aattcatcgt gatggcggcc cacctgatcc tgatcaagtc ccggatgctg gttcccgggg
     3301 accctgagca cgaggacgat gaagaggatc cccggaccga actggtcgcc cagttgatcg
     3361 agtaccagaa gttcaaggag gcggcccaca tcctcgaaga gagcgagagc ctctcgcggg
     3421 cggtcctcga acgcaaggcc ggacagatcg tgttcgactt tcccggggac gagtccaact
     3481 gggtggatgt caaacttttc gatcttgtca atgccttctc gcggatcatg tcccgcagtg
     3541 aaaaagaacc gccgagttat ccggtctttg acgatgtcga ggacaattat gatcccgagg
     3601 agaagatcac ccagatccgg accatgctcg aaatccattc gcggatcgag ttcaacgaga
     3661 tcatcacgcc ggcaacccgg cgcggcgaga tcattatcat ttttctcgcc ttgctgcaca
     3721 tgatcaaacg tggatatatt gttgtccggc agcaccggct ttttggtgat attaccattt
     3781 ttgcccgtaa tgccgctccg gacggagttg cctcggggat tgtcgaagaa cccgaagagg
     3841 atacgggcag tcaacaggga tcaggagatg gtgtgtgagc agggaagaaa aaaaggcctt
     3901 ggtcgaggcg cttctgttta tcgagaacga gccggttgaa tacgggaaac tggtcaagca
     3961 taccggtctc gaacagcagg agctctcggg cctcgtcgag gaactcaaga ccgagtacga
     4021 actcaggcag agcgggatcc agatcgtcga ggtcgccggc ggctgtcaga tgatcgccca
     4081 tccccggttc ggactggtcc tctcacagat ctatggcgtc aaaaacaaca acaagctctc
     4141 gcaaacggcc ctcgaggtgc tggccatcat cgcctacaaa cagccgatca ccaaaaccga
     4201 gatcgaagag attcgtggcg tttcctgcga gcgtgtgata aaattgctga tggagaagga
     4261 tctggtcgtg atcagtggac ggagggagac ccccggcaaa ccgctcgagt tcgggaccac
     4321 aaagaacttc ctcaaggtat tcggacttaa atccatcaac gatctcccaa aactccgcga
     4381 gatcaaagag atggagttcg agattcggga gaacggcgac aatgaatgag ccaatggaag
     4441 gcgcccgtct ggacatagat gttttgctgc aggatgcgga tacacttccc gcatttccct
     4501 ccatcgtcac cgaaattctc gcccgaattg atgatcccct ctccgttccg gatgatttta
     4561 ccgaaatcat cagccgggat caggtgatgg tcagcaaggt tctcaagttt gccaattccg
     4621 ccttttacgg atttccccgg accattgtta ccctcaaaga ggccgtcatc attcttggtc
     4681 tggataccct taaaagcatt gttttggcga tctccagcta ttctactctc aacgccaaca
     4741 acaaaggcta cgggctcgac aagggcggtc tctaccagca ttcgatctgc tgtgccatga
     4801 ttgcccggat cctggctaaa aagtacaaat ccgaaaacaa ggaaaagttt ttcatcgcgg
     4861 ccctgttgca tgacgtcggg aaactccttc ttgacccctg gctttcgcgc ttcaagaccg
     4921 agatgatcga tggcatccgg aaggggaacc ttgcaccctg ggaagccgaa cgccgggttc
     4981 tcggattcga tcatgccttt gtgggaagca agctgatcga gggctggaat cttcccgaag
     5041 tcctgattca tgtcacgcgc tatcaccata cccccgaaaa agctccgctg gaacatcgcg
     5101 ccatcgtcga gatgatccag gccgcaaaca tcatctcccg tcggatccgt ggtggcctgg
     5161 gtggcgaatt cattcccccc gaaggaaagg cgatggatga actgcaacat gtcctccccc
     5221 tggccgagga tgaggtccgt tccattgtcc gggaagttcg gacctacctc ctgcatatca
     5281 ataccttcat ctgaacccgg ggaaggcgtt catactgaat cccttgaaca gagtgccaat
     5341 cccttgctat acttggccgg atgagtgacg aacagcagac acttggaacg cttgagggac
     5401 ttcgctcgaa gatcgacgaa gtcgatcgtg gtattgctgc cctcctcgaa gagcggatgg
     5461 atcttgcgct ccagatcggg caggtcaagg cccgggaggg gctcccggtc tatcatcccg
     5521 agcgggaaca ctcggtcatt tcccggatcg tcgaacactt ccatgccggc cactttccgc
     5581 tggattccgc caaaaggatc tataccgaaa tagtcggagc ctgtcgtgat gcgcagcgcg
     5641 ccctgcgtgt ggcctatctc gggccacacg ccacgtattc acatatggct ctcgagagac
     5701 ggtttggcgg ttccgcactg gcgatacccc aggaaacgat cgccgatgtg ttttcggctg
     5761 tcatctccca aaacgccgat tgcggggttg ttcctgtcga aaactcgaac gagggaagtg
     5821 tggcccacac tcttgatctc ctggccgagg gcagggtcca tgtcatcggg gaagttctcc
     5881 agcccgtgca ccactgtctc ctctcgacac agaccacgat ggaccggatc cggcgtgtcc
     5941 ttgcacaccc ccaggcgctt gcccaatgcc ggcgctggat cagccggaat cttccgcacg
     6001 ccactgtcgt tgaaacatca agcaacggga aagccgccga gatcgcggcc tccgaagacg
     6061 gttcggccgc catcgccgcc aggattgccg ccaggctgta tcgtctcaac ctccttgccg
     6121 agagcatcga ggacaatgcg gacaatatca cgcgattctt catcatcggt gccaagccga
     6181 ccatgccaac cggaaaagac cggacctcac tggtaatcgc agtcaaggac agacccggca
     6241 tcctgcatga tcttctcgat gtctttgccc ggagaggaat caatatgtcg cggattgaaa
     6301 gccgcccttc tcggatcaag gcatgggaat acgttttttt tgtcgatgtc gacgggcatg
     6361 cccaggtcag ccccctctcg gacgcattga gtgaacttga tgcccatgtc aggaccctgc
     6421 gggtcatcgg ctcgtatcca aggtcggaag actgaatatg aacaatcact tcgtaatagc
     6481 cggtctgggc cttctgggcg gatccttgtc tctggcactc aagcggcgcg accctgaatg
     6541 ccggatacat gttcttgggc gggatcccga gcgactggag acagcacgaa gggaaggttt
     6601 ttgtgattcc tggagtgtcg attcgaagaa tcttccggcc ggtatccggg g
//
LOCUS       DAOSHE010000070         6573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10221_length_6573_cov_4.925437, whole genome shotgun sequence.
ACCESSION   DAOSHE010000070 DAOSHE010000000
VERSION     DAOSHE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10221_length_6573_cov_4.925437"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLD82_03145"
     CDS             complement(<1..641)
                     /locus_tag="PLD82_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG64417.1"
                     /translation="MNTEQTHGDGGDVILQAFHWNLVKTQGTGTVDGRDESWYAILKT
                     SIERIAASGCTVLYLPPPWRDDSAWESNGRHGGGEGYYWHDFSLDSRYGTKAELVALV
                     DAAHRNGLKVIVDIVLNHRDGGRMEADIWPWPGPCWRNGGADSGGAFMDGRFDLALNE
                     PMVQERIRRALEELSVDCGVDGWRWDYVWGYDPQDVVHWLGLTKKKEYISIGE"
     gene            718..1149
                     /locus_tag="PLD82_03150"
     CDS             718..1149
                     /locus_tag="PLD82_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG64418.1"
                     /translation="MSVTSSLPVSIADINYGQHMGNDRFLAFFHEARLNFLASVSASE
                     VDIGEDTGLIMTEANVRYLGQVVRGDLLETTVDVLEVKKSGFVLGYEMYRSQDGKRVA
                     SGTTALCAFKYAEGKIARLPGGFKQALEAHLRQPEGDRAGS"
     gene            complement(1166..2689)
                     /locus_tag="PLD82_03155"
     CDS             complement(1166..2689)
                     /locus_tag="PLD82_03155"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG64419.1"
                     /translation="MKNRVYIFDTTLRDGEQSPGASMSVREKLEIALQLERLGVDVIE
                     AGFPVSSPAQMEGVSLVASELRHVTVAALSRAVKTDLDAAAKALAKAKHGRIHTFIAT
                     SPIHMKHKLGKEPDQVLQMAVDAVRYAKGLAPEVEFSAEDASRSDYGFLSEIVQAVIE
                     AGASIINLPDTTGYAVPGEYMRLFKEVIEKTPNADKAIFSVHCHNDLGMAVANSLAGV
                     LGGARQVEVSVNGIGERAGNAALEEIVMALSTRKDSFQLETGIKTEEIFRTSKLLSST
                     IGFPIPANKAIVGKNAFAHESGIHQDGVLKERSTYEIMTPESIGRKSSDIVLGRHSGK
                     HGFTKKLAELGYAPDKDALEKAWLRFTEVADKKKEVFDDDLVAIIEDALHASVPETWK
                     LEYVNFTGGNTSIPTATVVLVRNGERFQEAATGDGPVDAIYGAINRIVNVPGVVLEQY
                     SLSAVTGGMDALGEASVSIRYQDRLYSGRAAATDVVLASCRAYVNAINRMLVPIRKV"
     gene            complement(2810..3829)
                     /gene="ilvC"
                     /locus_tag="PLD82_03160"
     CDS             complement(2810..3829)
                     /gene="ilvC"
                     /locus_tag="PLD82_03160"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOG64420.1"
                     /translation="MMKIYYEGDASLDAIGKKTIAVIGYGSQGRAQSLNLRDSGFNVV
                     VGLREGGVSWRNAEKDGWKPLTVAKAAEVADIIQILTPDETQGQTYKSEIASSMKSGK
                     TLMFSHGFNIHYNQIVPPKDVNVIMVAPKGPGHTVRSQFEKGSGVPGLIAIHQDVDGK
                     ARDIALAYAKGLGATRAGVIETTFREETETDLFGEQSVLCGGLANLILAGFETLTEAG
                     YAPEMAYFECLHEVKLITDLIFQGGLADMRYSVSNTAEYGDYVSGPRVINDESKKAMR
                     AVLKDIQDGTFARNWMLENMAGKPFFNAQRRTAAAHPIEEVGKKLRSMMKFSNDGKLI
                     TEERE"
     gene            4075..4656
                     /locus_tag="PLD82_03165"
     CDS             4075..4656
                     /locus_tag="PLD82_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG64421.1"
                     /translation="MKRREAAALDLELERRFERLYRQHYARMVSQVHYMVNDIEQAQE
                     IVQDVFVRLHRNLPTIATDELVPGYLFIACRNAIKNEYRRRGRIKSGYGKTVFVDMEK
                     TDFFFRNAETEEDAFIKREDTKAVLRSLAQLKLEDREIILMKEFGGQRYEEIAETLGT
                     TTTVVRGRLHKARQRLRKALLEPGKEIIDETRD"
     gene            4640..5098
                     /locus_tag="PLD82_03170"
     CDS             4640..5098
                     /locus_tag="PLD82_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG64422.1"
                     /translation="MRHATDRQLSALIDNELSEQKRVRIEEHCRTCAQCSKRLEEYKR
                     IQSTLSALPVLKLDPDFALTRVRARVREADQCEPLVVRSWYHPALAIAAAALVAIAVL
                     FAVPGEVREEDGGLDPKATLTQGPLDNCVAEQETDDIIRGYHDMKRLYYF"
     gene            5146..5676
                     /locus_tag="PLD82_03175"
     CDS             5146..5676
                     /locus_tag="PLD82_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64423.1"
                     /translation="MWRMILLTSLLVVLALPVSAQQRDVPANAEELTLLGNPKERMQK
                     INRLLREAGERVLEQKRMLDDAVARIIANERKMAASSLSGEERQKLRTANIAAFREIE
                     IIRERIARIQFDTAQEVYQIEQTAYHSMINDIAQKNAQIGTDPLAYWLAVRRMLLSPS
                     ELSSNLSENDLKAIYR"
     gene            5651..>6573
                     /locus_tag="PLD82_03180"
     CDS             5651..>6573
                     /locus_tag="PLD82_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64424.1"
                     /translation="MISKPSTGKIGLLVFLGCTLLTPAFLAGVSPLTRPVVHSPALGS
                     AIRLWAHLSRQDLWYTEAGPPDRRAAPGAVFFSLWKSSLANPQGGNRIADLGYSFAHP
                     LLDSKRQVVWYAQRVSDSNRDGVIDEQDGLSIIRLDLARNRPETVTDRDEISWPLSLD
                     PNTGRILAVVDGYYAILEADDGDIDERLARFDSRTSRGSRPFFDTHGKPWLMSQDAPA
                     LAITRNTLSPGQPVVLADPGSKVSIQIVGPGGKPHFRINAPDGKTVEVPAEQGLWPLS
                     IIDSTRIIWIDRGRIFAADHHQKRHEPLVLLP"
BASE COUNT         1397 a         2046 c         1781 g         1349 t
ORIGIN      
        1 tactcaccga tggagatgta ctccttcttt tttgtcagtc ccagccaatg gacgacatcc
       61 tggggatcgt agccccagac gtaatcccag cgccacccat cgacaccaca gtcgacggag
      121 agttcctcca acgccctgcg aatccgttcc tgaaccatcg gctcattgag ggccagatca
      181 aaacgcccat ccataaaagc gccaccactg tcggcaccac catttctcca acagggaccc
      241 ggccaaggcc agatatccgc ctccatccgg ccaccgtccc tgtggttgag cacaatatcc
      301 acaatcacct tgagtccgtt ccggtgggcg gcatccacca gcgcaaccaa ctccgccttt
      361 gtcccatacc gggaatccag cgagaagtca tgccagtaat agccttctcc cccgccatgc
      421 ctgccattgc tctcccaggc tgaatcatcc ctccagggtg gaggcagata gaggacggta
      481 cacccgcttg ccgcgatgcg ctcgatcgac gtcttgagaa tcgcatacca ggactcgtca
      541 cgcccgtcaa cggtccccgt accctgtgtc ttgaccaggt tccagtggaa agcctggagg
      601 atgacatctc ctccatcgcc atgtgtctgc tctgtgttca tggtctgagt atacgccctg
      661 ataaaaaaaa tgcaagactc gaatcgggta tgctatgctt cgttgtggag ggagtccatg
      721 tccgtgacaa gcagcctgcc cgtcagcatc gctgacatca attacggtca gcacatgggg
      781 aatgaccggt ttctggcctt cttccatgaa gccagactga actttctcgc cagcgtctcg
      841 gccagtgaag tcgacatcgg tgaagacacc ggcctgatca tgaccgaggc aaacgtccgc
      901 tatctcgggc aagtcgtccg gggagatctc ctcgaaacga cggtcgatgt cctcgaggtc
      961 aaaaaatccg gtttcgtcct gggctacgag atgtaccgca gccaggacgg gaaacgggtc
     1021 gccagcggga caacggcact ctgcgccttc aagtacgccg agggaaaaat cgcccgactc
     1081 ccgggcggat tcaaacaggc gctcgaagcg cacctgcgcc agccggaagg cgatcgggcc
     1141 ggaagctgat cacccccgac gggactcaga ccttgcggat cgggacgagc atccggttga
     1201 tggcattgac ataggcccga cagctcgcca gaacaacatc cgtggccgcc gcccttccgg
     1261 aatagagccg gtcctgatac cggatgctga cggacgcctc acccagggca tccataccac
     1321 cggtgactgc cgacagcgag tactgctcaa ggacaacgcc cgggacattc acgatccggt
     1381 tgatggcacc atagatcgca tccacaggac cgtctcccgt tgccgcctcc tgaaaacgct
     1441 ccccgttacg gacaaggacg acggtggcag tcgggatgga ggtattcccc ccggtgaagt
     1501 tcacatactc cagcttccag gtctcgggaa cactggcgtg gagagcgtcc tcgatgatgg
     1561 cgaccagatc gtcatcaaag acttccttct ttttgtcggc gacctctgta aaccgcagcc
     1621 aggccttctc cagggcatcc ttgtccggcg catacccgag ttcggccagt ttcttggtaa
     1681 acccgtgctt ccctgaatgc cggcccagga caatatcact cgacttgcgc ccgatggact
     1741 cgggggtcat gatctcatag gtactgcgct ccttgagaac accatcctga tggatgcctg
     1801 actcgtgggc aaaggcattc tttcccacaa tcgccttgtt ggccggaatg gggaacccga
     1861 tcgtcgacga gaggagtttg ctcgtgcgga agatctcttc cgtcttgatt ccggtttcca
     1921 gctggaaact gtccttccgc gtggagaggg ccatgacgat ctcctccaat gcagcgttcc
     1981 cggcccgttc cccgatcccg ttgaccgaaa cctcaacctg cctggcaccg cccaggacgc
     2041 cggccagact gttggcgacg gccatcccca gatcgttatg gcaatggacc gaaaaaatcg
     2101 ccttgtccgc attgggggtt ttttcaatca cttctttaaa caacctcata tactccccgg
     2161 ggacggcgta tcccgttgtg tccggaagat tgatgatgct cgcccccgcc tcgatcacgg
     2221 cctgaacgat ctcggagaga aacccgtagt ccgagcggga ggcatcctcg gcggagaatt
     2281 caacctcggg tgccaagcct ttggcgtatc tgaccgcatc cacggccatc tgcagaacct
     2341 gatccggctc cttccccagc ttgtgcttca tgtggatcgg agatgtcgcg atgaaggtgt
     2401 ggatccggcc gtgcttggct ttggccagtg ccttggccgc cgcatccaga tcggttttca
     2461 ccgcccggga gagagccgca accgtcacat ggcgcagttc ggacgccacc agggagaccc
     2521 cctccatctg ggccggactg gagaccggaa aacccgcctc gatgacatcc acacccagcc
     2581 gctcgagttg cagcgcaatc tccagttttt cacggacact catgcttgcg ccgggagact
     2641 gctcaccgtc acgcagggtt gtatcgaaaa tgtacactct gttcttcatg ttgaactcct
     2701 ttcagcttct cgtattggat acgccacagg cggagcttga acaaaaaaag gcagcctccg
     2761 cgctgtggcg ggactgcctc tttgaaggca tgcctccctg ggtgcgcggt tactcgcgtt
     2821 cctcggtgat tagcttgccg tcgttggaga atttcatcat gctgcgcagc tttttcccga
     2881 cttcttcgat gggatgggca gcggctgtcc tgcgctgggc gttgaagaaa ggcttgccag
     2941 ccatgttctc cagcatccag ttgcgggcaa aggtcccgtc ctggatatcc ttgagaacgg
     3001 cacgcatggc ttttttggac tcgtcattga tgacgcgcgg accggagaca tagtcgccgt
     3061 attccgcggt gttggagacg gagtagcgca tatcggccaa tccgccctgg aaaatcaggt
     3121 ccgtaatcag cttgacctcg tggagacatt cgaaataggc catctccggt gcgtatccgg
     3181 cctcggtcag tgtctcgaaa ccggccagga tcagattcgc caaacctcca cagaggaccg
     3241 actgttcacc aaaaagatcc gtctcggtct cctcacggaa tgtcgtttcg atcacaccgg
     3301 ctctggtggc acccagaccc ttggcatacg ccaaagcgat gtcgcgggcc tttccgtcaa
     3361 catcctggtg aatggcgatc agccccggga ctccgctccc cttctcgaac tgggaacgaa
     3421 ccgtgtgtcc gggccccttg ggagcgacca tgatcacgtt gacatccttg ggaggaacga
     3481 tctggttgta atggatattg aagccatgcg agaacatcag ggtcttccct gacttcatgg
     3541 aagaagcgat ctcgctcttg tacgtctggc cctgggtctc atccggcgtc aggatctgga
     3601 tgatatcggc cacctcggca gccttggcca ccgtcagagg cttccagccg tctttttcgg
     3661 cattccgcca ggacacacca ccctcgcgga gaccaacaac aacgttgaaa ccgctgtccc
     3721 gcagattgag ggactgtgcc cgcccctggc ttccatagcc gattaccgct attgtttttt
     3781 tgccaatcgc atccagactg gcgtcaccct cgtaatagat cttcatcaat cggtctccaa
     3841 ttgcatttct ctgtttcgta aacataccct gtccgccgaa aaagtcaagt ttcttgagga
     3901 atggggtgcc ggccaaaggt tcccttgggg cggatctgtt tttatggagt ccacacgatt
     3961 tcgtgttttt cattgagtct cgcagccttg agtgctatca tgtcacgagg gcgcaggtcc
     4021 tccggtgcac tcctccggga gggcagtgaa cataaatcag gaagtggtgt cttgatgaag
     4081 cgacgggaag cggctgcgct cgacctggaa cttgagcgga gatttgagcg gttgtacagg
     4141 cagcactacg ctcgaatggt cagtcaagtg cattacatgg tcaacgatat cgagcaggcc
     4201 caggaaattg ttcaggatgt cttcgtccgc ctgcaccgca acctgccgac cattgcgact
     4261 gacgagcttg ttccggggta ccttttcatc gcctgccgca atgcgatcaa aaacgagtac
     4321 cgccgccgcg gacgcatcaa gagcggctac gggaagacgg tctttgtgga tatggagaag
     4381 acggattttt tcttcaggaa tgccgaaact gaggaagacg ccttcatcaa gcgcgaagac
     4441 accaaggcgg tgctgcgctc tctggcgcaa ctcaagcttg aggatcgtga gatcatcctc
     4501 atgaaggagt tcggagggca gcggtacgaa gaaatagcgg agacactcgg gacaacaaca
     4561 accgttgtcc ggggacgcct ccacaaggcc agacagcgtc ttcgcaaggc gcttctggaa
     4621 ccaggaaagg agatcataga tgagacacgc gactgacaga caactctccg ccctgatcga
     4681 caacgaattg tccgagcaga aaagggtgcg cattgaggaa cactgccgca cctgcgcaca
     4741 gtgcagcaaa cgtcttgagg aatacaaaag gattcagtcc accctgtcgg cactccccgt
     4801 cctcaaactt gatccggatt tcgcactgac cagagtccgc gccagggtcc gcgaagccga
     4861 ccaatgcgag ccactggttg tccgctcctg gtaccatccg gcgctggcga tagcagcggc
     4921 tgcccttgtt gcgatagcag ttctttttgc ggtaccgggc gaggtcagag aggaagacgg
     4981 gggacttgac cccaaagcca ccctcacgca gggtcccctt gacaactgtg tcgccgaaca
     5041 ggaaaccgat gacatcatcc gcggctacca tgacatgaaa cggctctatt acttttgacc
     5101 ggcaccctct acggcatccg gcatgaacag cgaggcaata atcccatgtg gcgaatgata
     5161 ctcttgacca gccttctggt tgtgctggca ctcccggtgt ctgcacaaca gcgtgatgta
     5221 ccggccaacg ccgaggaatt gaccctcctc gggaatccca aagagcggat gcagaagatc
     5281 aaccgtcttt tgcgcgaggc cggcgaacgc gtcctcgagc aaaagcgcat gctggacgat
     5341 gccgtcgccc gaatcatcgc caacgagcgc aagatggccg ccagcagtct ctccggcgag
     5401 gaacgacaga aattgcgcac ggcaaacatt gccgctttca gggaaatcga gatcatccgt
     5461 gaacggatcg cgcgaatcca gtttgatacg gcgcaggagg tgtaccagat cgagcagacg
     5521 gcataccact caatgatcaa cgacattgcg caaaaaaacg cccagatcgg gaccgacccg
     5581 ctcgcctact ggctcgcggt gcgccggatg ctcctctccc cgtccgaact ctcttcaaac
     5641 ctgtccgaaa atgatctcaa agccatctac cggtaagatc ggactcctcg ttttcctcgg
     5701 ttgcaccctc ctgacaccgg ccttcttggc cggtgtcagt cccttgaccc ggccggtcgt
     5761 ccattcaccg gcgctcggtt ccgccatccg tctctgggcc catctctccc gccaggatct
     5821 ctggtacacc gaagccggcc cacccgatcg cagggcagcc cccggtgctg tctttttcag
     5881 cctctggaaa agcagcctgg ccaaccccca aggcgggaat cggattgccg atcttggata
     5941 cagttttgcc caccccctgc ttgacagcaa gcggcaggtc gtctggtatg cccaacgcgt
     6001 gtccgactcc aatcgggatg gcgttattga tgaacaggac gggctctcca tcatccgtct
     6061 tgacctggcc cggaatcgcc cggagacagt gactgaccgg gacgagattt cctggcccct
     6121 ctcgctcgac ccgaataccg gccggatcct ggcagtcgtg gacggatact acgcgattct
     6181 cgaggctgat gacggagaca tcgatgaacg cctggcacga tttgacagcc ggacttcccg
     6241 cggatcccgt ccctttttcg acacccatgg caaaccatgg ctcatgtcgc aggatgcccc
     6301 ggcgttggcg atcacccgga atacactctc cccgggacaa ccggtggtgc tggccgatcc
     6361 cggctcgaaa gtctccatcc agattgtcgg acccggcgga aagccgcatt tcaggatcaa
     6421 cgccccggac ggcaaaaccg tcgaagtccc ggctgaacag ggactctggc ccctctccat
     6481 cattgacagc acacggatca tctggatcga tcgggggcgg atcttcgccg ccgatcacca
     6541 tcaaaagcgc catgaaccgc ttgtgctcct ccc
//
LOCUS       DAOSHE010000071         6523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10339_length_6523_cov_4.127087, whole genome shotgun sequence.
ACCESSION   DAOSHE010000071 DAOSHE010000000
VERSION     DAOSHE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10339_length_6523_cov_4.127087"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..771
                     /locus_tag="PLD82_03185"
     CDS             283..771
                     /locus_tag="PLD82_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOG64425.1"
                     /translation="MIGSKMLDAINEQIHRELYSEYLYLAMAAWFRTEGLDGFGHFFE
                     KQAQEEHAHAMKFYHHVFERGGRVVLKAIEAPPADFTGVEAVIEQSLEHERFVTRSIH
                     DLVDLAAAEKDHASSSFLTWYVDEQVEEEAHMEEILTKIKMIKGNPQGLLMMDGKLGA
                     RS"
     gene            1061..1855
                     /locus_tag="PLD82_03190"
     CDS             1061..1855
                     /locus_tag="PLD82_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64426.1"
                     /translation="MMRGILLPMGLLACFLMVGCRSEGESALAEGNTLLNSTDPADWE
                     RAAEAFERSIETEVKARSQLVRAWSKVGVRKMNEADKMTVKREDEMQIHLIGGTAKRI
                     GMMAAEYELYTAAISNLQLAARALPNDKMTWYYLGLSWGQISRGQPDGPRATEYLEKS
                     DDAFKRAIKLDADFRNALYGRGIVLIIKKDTDEAERVLQHLVSLEPKEARGYFALGRV
                     YYDRKEYQKAENVYHLLREMIPEKSPKRGLIEENLRKLEIMPKVRP"
     gene            1852..2928
                     /locus_tag="PLD82_03195"
     CDS             1852..2928
                     /locus_tag="PLD82_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG64427.1"
                     /translation="MTRCERLALALLFGPGSSSAALLLSADPDWNPFVMEEAGMARAS
                     LPAMRRIMRKIFCEGEAALERARAIEERSARLGLQILLPGDPDWPVQCNALYAPPLAL
                     YLRGDASILTKRSTTMIGTRQPTARALLLACDLAAILAKSGTPVLSGGAFGIDAAAHA
                     GALDAGGKTIAVLAGGLDRLHPREHALLFGRIAERGALVSESPPGTPAQGWRFPVRNR
                     LMAALGSELILVQAPLRSGTHSTVVEALALGREVRVCPWSPECVEGAGCMQLAGMGAE
                     LIVTGRELSDQVDGEMSQIESRGDEVLCAVHAAILQGFATTDDLVALTGLPVETVMTQ
                     CMRLELEDRIRRYGTLGYGPGKAA"
     gene            3032..5146
                     /gene="topA"
                     /locus_tag="PLD82_03200"
     CDS             3032..5146
                     /gene="topA"
                     /locus_tag="PLD82_03200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG64428.1"
                     /translation="MAVRTDKAERKILVIVESPTKANTISKSLGKEYIVEASMGHVID
                     LPKSRMAVNVENGFEPDYITVRGRGKVLNTLKRLAGKSKEVLLAADEDREGEAISFHL
                     MNELTKKYPGLPINRIVFHEITPDVIREAAMHPVGINMPKVMAQKARRVLDRLVGYNI
                     SPILWEKVKKGLSAGRVQSVALRMICEREKTITEFVPAEYWRVKLGLSAGKHAVVAEL
                     SRIRGEKAEVTSRQAVDSILDAVRGKAFSCTNVRTGKSTRRPTPPFTTSKMQQDAANK
                     LGFTSDRTMRIAQQLYEGIELPGGPVGLITYMRTDSTRVSDLAIEQVRRYIDEKYGAD
                     TLPEIPVSYANKKGSQNAHEAIRPTDVFRHPDSIKEHLSRDQYRLYKLVWSKFLASQM
                     RPAELETTTIEITNGEYLFTVSGSRVVVQGYQKVYSEESEEEARTPKVPKIAVGDALT
                     LTDDTLSTQHFTQPPPRYTDASIIKALDETGIGRPSTFASIVTTLIKRYYIVRQTKQL
                     VPSELGTLINGLLVANFPKLIDVDFTASMEEQLDEIEDERLEWKALLGEFYKDFLPML
                     DTARQEIQSMKNVFDEVTDHTCEKCGKPMVKKLGRFGYFLACSGFPECRSTQPIPLAK
                     CPMPDCGGNIVAKKGRRGRMFYGCSNYKEDGTGCGFVSWDKPASKPCPKCGKLLVERR
                     TKESGFVLVCHDEACGYSEKAE"
     gene            5146..6042
                     /locus_tag="PLD82_03205"
     CDS             5146..6042
                     /locus_tag="PLD82_03205"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOG64429.1"
                     /translation="MDSGVADYLVWLEASRNLGHASIEAYRKDLEGFSAFLESEGVGH
                     PVAIDVRFVRAWLHNLHGHGFSRTSVARKLSSLRGYLDWLCRRGVLPSNPAALVSTPK
                     RGTRLPRHLHENEILPLVDELSALEDPSGRQDAALCILLYSTGMRVSELCSLETGDIQ
                     PYDDTIKVTGKGRKERVIPVGMAARKAVSRWLEVRGKAGRNAPLFVNARGTAMQPRSV
                     RYRLARLVIRMAQARHISPHMLRHSFATHLLDHGADLRSVQELLGHASLSTTQIYTHL
                     SKQRLREVYDACHPHAKKDGED"
     gene            6039..>6523
                     /gene="hslV"
                     /locus_tag="PLD82_03210"
     CDS             6039..>6523
                     /gene="hslV"
                     /locus_tag="PLD82_03210"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOG64430.1"
                     /translation="MMESIHATTIICVRKSGRVVMAGDGQVTAGSTIMKAKAKKVRTM
                     YGGRILAGFAGSAADAFTLFELFEKKVEEFHGDLARAAVELARYWRSDKALRTLDAMM
                     LVADSKRTFLLSGNGDVIEPDTGVIGIGSGGSYAYAAARALHDATDLDAPATARKAKG
                     IA"
BASE COUNT         1424 a         1741 c         2025 g         1333 t
ORIGIN      
        1 caaatgtgag gcaacgcatc tgaccttcgg ttaccttttt caatgatgca acgcgattat
       61 gcaagcggta acataacact cgataatgta tagagataaa aatcatggat ccggcaggaa
      121 atattcagca tggcacggaa tatgctatta taacgagtgt gttgcccctc tcttgtgagg
      181 ccgggaccgg gcgttgccct cccggtctct ttttgtcttt atcgaagaat tgcactatct
      241 tgaaaatacc ctgtagggag tacgtgactg gaggtccgca gtatgattgg cagcaagatg
      301 cttgacgcga ttaacgaaca gattcaccgt gagttgtatt cggaatatct ctacctcgcc
      361 atggcagcct ggtttcgcac cgaaggcctg gatggttttg gtcatttttt cgagaaacaa
      421 gcccaggaag agcatgcgca cgccatgaag ttttaccacc atgtttttga gaggggcggg
      481 cgtgtggttc tcaaggccat cgaggctcca ccggcagatt ttacgggtgt cgaagccgtg
      541 attgagcagt ctctcgagca tgagcggttt gtgacccgct ccatccatga cctggtcgac
      601 ctggcagcgg cggaaaagga tcatgccagc tcgagcttcc tgacctggta cgtcgatgaa
      661 caggtcgagg aagaggcgca tatggaggag attctgacca agatcaagat gatcaagggg
      721 aatccccagg gacttctcat gatggacggg aagctcggcg ctcgctcctg attgaagagt
      781 tcgagaccgg tttggatgtt ctggcacagg gagtcattcc tgtgcttttt ttatattcac
      841 cggccgaggc aggagctctg ctcgacggtc ccgcaggcag gatgtattgt agaagacggt
      901 ccacatggaa gtgtgggatg gtcatactcc ggattgtacc gggcatccgg gttggtcccc
      961 ggcgtccctg aaagattttg ctgtttttcg gttcacgctg caaaatttgc ggggggtggg
     1021 ccgttctgat atgataaaga tgtgaaaagg ctggaggaag atgatgcgtg gaatactgct
     1081 cccgatgggg ttgctggcgt gtttcctgat ggtgggttgt cgcagtgagg gggagagtgc
     1141 tctcgccgag gggaataccc tcttgaacag tacggatccg gcggattggg agcgggcagc
     1201 ggaagccttt gaacgatcga tcgagaccga agtcaaggcg cgcagccaac ttgtccgggc
     1261 atggtccaag gtcggtgtcc gcaagatgaa cgaagccgat aaaatgacag tcaaacgcga
     1321 ggatgagatg cagatccatc tgatcggcgg gacagccaag cggatcggca tgatggcggc
     1381 cgaatacgaa ttgtataccg cagcgatcag caatcttcag cttgccgccc gggcactccc
     1441 gaatgacaag atgacctggt attacctcgg actctcctgg gggcagatct cccggggaca
     1501 acccgacggc ccccgggcca cggaatacct cgaaaaatcg gacgatgcct tcaagcgggc
     1561 catcaagctg gacgctgatt tccggaatgc cctctacggg aggggaatcg ttttgatcat
     1621 caaaaaagac acggatgagg ccgaacgggt cttgcagcac cttgtcagtc ttgaaccgaa
     1681 ggaggcgcgc gggtattttg ccctcggtcg ggtctattat gacaggaagg aataccagaa
     1741 ggcggagaat gtctatcatc tcctgcgcga gatgattccc gaaaaatctc ccaagcgtgg
     1801 cctgatcgaa gagaatctcc gcaaactcga aatcatgccg aaggtgcggc catgacgaga
     1861 tgcgagcgtt tggcattggc cctcctcttc ggtccgggct cgtcttccgc ggcgctcctt
     1921 ctgtcggcag atccggactg gaatccgttc gtgatggagg aggccggaat ggcgcgggca
     1981 tctctgccag ccatgcgacg gatcatgcgg aaaatcttct gtgagggaga agcggcactt
     2041 gagcgggccc gggccatcga ggagcgttcg gccaggctcg ggttgcagat tcttctgccg
     2101 ggagatccgg actggccggt tcagtgcaat gcgctctatg ctccgcctct ggcgctctat
     2161 cttcgggggg acgcctcgat tctcacgaaa cgcagtacaa caatgatcgg aacccgccag
     2221 ccgacggcga gggcactctt gctggcctgc gacctggcgg ccattctcgc aaaaagtgga
     2281 acaccggttc tctccggtgg tgcgttcggg atcgatgcgg ccgcacatgc cggcgccctg
     2341 gatgcgggcg gaaagacaat cgccgttctt gctggtgggc tcgacaggct ccatccccgg
     2401 gagcatgccc tcctcttcgg aagaatcgcc gaacgggggg ccctggtctc ggaatcgcca
     2461 cccgggacac cggcacaagg ctggcggttt cccgttcgca acaggctgat ggctgccctg
     2521 ggttccgaac tgatcctcgt ccaggcgccc ctgcggtcgg ggacgcacag taccgtggtc
     2581 gaggcattgg cactggggcg tgaggtccgg gtctgtccct ggtctccgga gtgtgtcgag
     2641 ggcgcagggt gcatgcagct tgccgggatg ggggctgaac tgatcgtgac cgggcgggaa
     2701 ctctcagatc aggtggacgg ggagatgtcg cagattgaat cgcgtggtga tgaagtcttg
     2761 tgcgcggtac acgcggccat cctgcaggga tttgccacga cggacgatct ggttgccttg
     2821 accgggctgc cagtcgagac cgtcatgacg caatgcatgc gccttgaact cgaggaccgg
     2881 atccggcgct atggcaccct cgggtacggt ccgggaaaag ccgcctgaaa aaaaatcttt
     2941 cacgcaacac atataaatgt agattttcac gaaatgaccc ccatcagacc tgtcgatggc
     3001 ggagaggcaa cacggaacaa aggcggacca gatggcagta cgtactgaca aggcggagcg
     3061 gaagattctt gttattgtcg agtcgcccac aaaggccaac acaatcagca agagtctggg
     3121 caaggagtac attgtcgagg ccagcatggg acatgtcatc gatctcccca aaagccggat
     3181 ggcggtcaat gtcgagaatg gattcgagcc ggattacatt acggtgcgcg gtcgggggaa
     3241 ggtcctcaac accctcaagc gtcttgcggg caagtccaaa gaagtcttgc tggcggctga
     3301 cgaagaccgc gagggagagg ccatctcgtt ccatttgatg aacgagttga caaaaaaata
     3361 tccgggtctt ccgatcaaca ggatcgtgtt tcacgagatt accccggatg tgatccggga
     3421 agcggccatg catcccgtcg gaatcaatat gcccaaagtg atggcccaaa aggcgagacg
     3481 tgtgctggac aggctggtcg gatacaatat cagtccgatc ctctgggaaa aggtcaaaaa
     3541 gggtctttcc gccggccgtg tccagagtgt cgcactgcgc atgatctgcg aacgtgaaaa
     3601 aacgatcacc gaatttgttc cggccgaata ctggcgggtc aagctcgggt tgagtgccgg
     3661 gaaacatgct gtggtggcgg aactctcccg gatacgggga gagaaggcgg aagtgaccag
     3721 ccgccaggcg gttgacagta ttctcgatgc cgtccgcgga aaagcctttt cttgtacgaa
     3781 tgtccggaca ggaaagagta cccgccgtcc gactccgccg ttcaccacca gcaagatgca
     3841 gcaggatgcg gccaacaagc tggggtttac ctcggaccgg accatgagga tcgcccagca
     3901 gttgtacgaa ggaatcgagc ttccgggcgg tccggtcggt ctgatcacct atatgcgtac
     3961 cgattccacg cgtgtgtccg accttgcgat cgagcaggtc cggcgctata tcgacgagaa
     4021 atacggtgcg gatacccttc cggaaatccc tgtcagctat gccaataaaa aggggagcca
     4081 aaacgctcac gaggcgatcc ggccgacgga cgttttccgg catccggatt cgatcaagga
     4141 gcatctctcc agggatcagt accgcctgta caagctggtc tggtccaaat ttcttgcatc
     4201 ccagatgcgg ccagccgagc tcgagacgac aaccatcgag atcacgaacg gggagtatct
     4261 ctttaccgtt tccggttccc gtgtggttgt gcaggggtac cagaaggtgt acagcgagga
     4321 gagtgaggag gaggcccgga cgcccaaggt tcccaagatt gcagtcggcg atgccttgac
     4381 gctgacggat gataccctct cgacccagca cttcacccag ccaccaccgc gctacaccga
     4441 tgccagcatc atcaaggctc ttgacgagac cggaatcggc cggccgagta cctttgcttc
     4501 gatcgtgacg acgctcatca aacggtatta catcgtccgt cagaccaagc agctcgttcc
     4561 gtccgaactc gggaccttga tcaacggatt gctggttgcc aatttcccga aactgatcga
     4621 tgtcgatttt acggcatcca tggaagaaca actggacgag atcgaggacg agcggctgga
     4681 atggaaggcg cttttgggcg agttctacaa ggatttcctt ccgatgctcg atacggcccg
     4741 ccaggaaatc cagtcaatga agaatgtctt tgacgaagtg accgaccaca cctgcgagaa
     4801 gtgcggaaag ccgatggtca agaagctcgg gcggttcggg tattttctgg cctgttccgg
     4861 gtttccggag tgccggagta cccagccgat tcccctcgcg aaatgcccca tgccggattg
     4921 cggtggaaat atcgttgcca aaaagggacg caggggccgg atgttctatg ggtgctccaa
     4981 ctataaagaa gatggaacgg gctgcggatt cgtctcctgg gacaagccgg cgtccaagcc
     5041 ctgcccgaag tgcgggaagc ttttggttga gcgccggaca aaagagtccg gatttgtgtt
     5101 ggtctgccat gatgaggcat gcgggtacag cgaaaaggcg gagtgatgga ttcgggcgtc
     5161 gcagactacc tcgtgtggct tgaggcttcg cgcaatcttg gtcatgcgtc cattgaggcc
     5221 tatcggaagg atctggaggg gttctctgcc tttctggaga gcgagggcgt cggccatccg
     5281 gtcgccatcg atgtccggtt tgtccgggcc tggctccaca atctgcatgg acacgggttt
     5341 tcaagaacgt cggtggcgcg aaagctctcc tcactccgcg ggtatctcga ctggctttgc
     5401 cggcgcgggg tcttgccgtc aaatccggct gcgcttgtct cgacccctaa gcgcgggacc
     5461 cgtctgccgc gacatctcca tgaaaacgag atcctgccgc tggttgacga actctccgcg
     5521 ctggaagacc cgtccggacg acaggatgca gccctctgca tccttctcta ttcgacagga
     5581 atgcgtgttt cggaactctg cagccttgag acgggggaca tccagcccta tgacgatacc
     5641 atcaaggtca ccggaaaggg ccgcaaggag cgggtgatcc cggtcgggat ggcggcgcgc
     5701 aaggccgttt cacgctggct ggaggtcagg ggcaaggccg gaaggaatgc accgctcttt
     5761 gtcaatgcac ggggaacagc gatgcaacca cgaagtgtcc gctatcgcct ggcgagactg
     5821 gtcatccgga tggcgcaagc ccgccatatc tcgccccaca tgctgcgcca ttcgtttgcc
     5881 acgcaccttc tggaccatgg agccgacctg cggagtgtgc aggagctttt ggggcatgcc
     5941 tccctctcga caacccagat ctatacacac ctttcaaagc aacggcttcg tgaggtctat
     6001 gatgcctgtc atcctcacgc caaaaaagat ggagaagact gatggaatcg atacacgcga
     6061 caacgatcat ctgtgtccgc aagagcgggc gggttgtgat ggccggggac ggtcaggtga
     6121 ccgccggaag caccatcatg aaggccaagg ccaaaaaggt gcgcacgatg tatggcggac
     6181 ggattctggc cggttttgcc gggagtgctg ccgatgcctt caccctgttt gaactcttcg
     6241 agaagaaggt cgaggagttc cacggcgatc ttgccagagc ggcagtggaa ctggcgaggt
     6301 attggcggtc ggacaaggcc ctgcggaccc tggatgcgat gatgcttgtg gccgacagca
     6361 agcggacttt tttgctttcg ggcaacgggg atgtgatcga accggatacc ggggtgatcg
     6421 gaatcggatc gggtggctcc tatgcctatg ccgcagcccg ggcactgcat gatgccaccg
     6481 atcttgatgc cccggccact gcccggaaag cgaagggaat cgc
//
LOCUS       DAOSHE010000072         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10353_length_6512_cov_4.664705, whole genome shotgun sequence.
ACCESSION   DAOSHE010000072 DAOSHE010000000
VERSION     DAOSHE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10353_length_6512_cov_4.664705"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="tig"
                     /locus_tag="PLD82_03215"
     CDS             complement(<1..950)
                     /gene="tig"
                     /locus_tag="PLD82_03215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG64431.1"
                     /translation="MSITAEMHKQPKSSVRLDIAIGNEEVRAAFDKAYREVAAKAHIK
                     GFRPGKAPVGIIRMKFGHAISHEVVEKLVQEAYSQAIKEHALQPVGQGRVTSEIPELG
                     ETDNLTLTMEVDVYPEFTVPEFKGIAVSKKSYQVTDADVDAELARLSERFTRFEDKAE
                     GTLESEDLAVVDYQVSLDGTPVEKLSREKYSYDLKTGASFPDFQDGLRGKKSGDTFEI
                     PGKIPEAFPDKEIAGKDVIFKGSIVKMQKRVAPVLDDEFAAKISDKKTMSEFRAMLLE
                     NMQDHARHHESEEVQNSILEQLVAKTSVELPEALVEMQLEG"
     gene            1199..2458
                     /locus_tag="PLD82_03220"
     CDS             1199..2458
                     /locus_tag="PLD82_03220"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG64432.1"
                     /translation="MSHNADIQPLEIVSRQIDAVNEILRLPDDEIAILKKPHRTLEVN
                     FPVVMDDGHIRVFTGFRVQHSLVRGPAKGGIRYSPDVTREEVQALATWMTFKCAVVNL
                     PYGGAKGGVIVDVTKISERELERLTRRFTSEIGIIIGPDSDIPAPDMNTNAKIMGWMM
                     DTYSMNKGHSIPGVVTGKPIELDGSLGRAAATGRGVSIVLNEFMKLKSLDLTKMRVAV
                     QGYGNVGRWAAKLIAALGAKVVAISDIKGGIHNPDGLDLADVENWTDTHKFLEGYPKA
                     RAISNAELLATDCDILVPAATEAVINRENAHTVQARYIIEGANGPTTPDAENILVKSG
                     TTIIPDILANAGGVIVSYFEWVQNIMTFFWDEDDINGKLMQALQRAFKDVETIHEQYQ
                     GITYRQAAYVLALSRETKARRMRGIYP"
     gene            2728..3228
                     /locus_tag="PLD82_03225"
     CDS             2728..3228
                     /locus_tag="PLD82_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOG64433.1"
                     /translation="MGKLGKEIVKLDVDILLKKLNSALADEWLAYYQYWIGAKVVKGP
                     MKDAVISELDIHATEELGHATLIAARIVQLGGTPVLSPDEWAKVAGCGYAAPVDPYVA
                     TVLDQNIKGEQCAIRTYSELLDFTRDKDPVTYNMVLGILSDEVEHEEDLASLAEDLEM
                     MLEHRK"
     gene            complement(3272..4030)
                     /locus_tag="PLD82_03230"
     CDS             complement(3272..4030)
                     /locus_tag="PLD82_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG64434.1"
                     /translation="MKLISWNVNGLRAVQDKGFFDFFHEYKPDILCLQETRLGPELAD
                     TSFAVPDGYHAHWVGAKKKGYSGVGILSKEMPLSWQEGLGDPQFDDEGRTLTAEYPEY
                     FVVAAYFPNSQHELLRLDYKMAYNRAFLAFCEKRRKKKPVIFCGDLNVAHEEIDLANP
                     KSNTMNPGFYIDERNWFTGMLEAGYVDTFRAQHPEPDQYSWWSYRFNARKKNIGWRID
                     YVVVSKELAPRVNESFILQSVMGSDHCPVGIVFS"
     gene            4495..>6512
                     /locus_tag="PLD82_03235"
     CDS             4495..>6512
                     /locus_tag="PLD82_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64435.1"
                     /translation="MNLHKAKSLRVKLTLYPALIFIVVGLVGTLVNLQIAGDLFEDVG
                     KAYTQEILKAKLSEFSQTFDRNIDTSQYMSENPILRNWMKNEDDRGLKDMAMENFRKL
                     RILLKDTDTFAAVDTSKSYYVNGNFLNTLSRDIAADLWYWTSSKKRELGIDISYNPQL
                     LTTKLWVYVPVWDEDRLLGVTGTGLDVSKMIKQMVSNVSKGAYVILFNDRGHVKAHIK
                     EEYIDNRTIFYLTGEDGNRIRGIMKRLDEAKDQSIISEFIDYDGKSHMGSFSLINLRS
                     TNWYILVLMDINALLYSIFVPFILLLVISLMLILGSLLILINRIVLHPLGVIDSNLAK
                     IMNKDFDVKISLATGDEFTMVANTINMMTANIKEYTEHLEDMVAQRTRELQQAFEEVN
                     ALKVQQDGDYFLTSLLINPLLVKEVRSETVSVDFHISQKKKFTFKKRNGEIGGDVCIA
                     REIVLMDKRYLAFANGDAMGKSLQGAGGALVMGVVFNAYINRTPLFASNKTPEAWLTE
                     IYEELNRVFVSFDGSMLISAVIGLLDEETGAMYYLNAEHPWTVLYRGGNAAFTEEELT
                     LRKIGTVGFENKVVIHMQQLEQGDVIYLGSDGRDDVLIGHDEESGQRIINEDETRFLR
                     CIEESNGNLEGVVSAVERSAELTDDFTLIRIEWKKAPLLVPPDYEAVRS"
BASE COUNT         1474 a         1696 c         1846 g         1496 t
ORIGIN      
        1 ccttcgagct gcatttccac gagggcctcg gggagttcaa ccgaggtttt tgccacaagt
       61 tgctcgagaa ttgaattctg aacttcctcg gactcgtgat ggcgcgcatg gtcctgcata
      121 ttttcgagca acatggcgcg aaactcggac atggtttttt tgtccgagat cttggcggca
      181 aactcgtcat cgagaaccgg ggcaacacgc ttctgcatct tgacgatgct ccccttgaaa
      241 atcacgtctt tgcccgcaat ctccttgtcc gggaaagcct ccggaatctt gccgggaatc
      301 tcgaatgtat ctccggactt cttgccgcgc agtccatcct ggaagtccgg gaaacttgcg
      361 ccggttttaa gatcgtagct gtatttttcg cgggaaagtt tttcgacagg tgttccatca
      421 agcgagacct gataatccac gacggccagg tcctcggact cgagggtccc ctctgccttg
      481 tcctcaaaac gggtaaagcg ttcggaaaga cgagccagtt cagcatcgac gtcggcgtcg
      541 gttacttggt aacttttttt ggaaactgcg attcctttga attcggggac agtgaattcg
      601 gggtagacat ccacctccat ggtgagtgtg aggttgtcag tttctccaag ctctgggatt
      661 tcacttgtga cgcgaccctg tccgaccggc tggagtgcgt gctccttgat agcctggcta
      721 taggcttcct gcacaagttt ttcgacgact tcatgtgaaa tggcgtggcc gaatttcatg
      781 cggatgatgc ccacgggtgc cttgcccggc ctgaagccct tgatatgggc tttggcggcg
      841 acttcgcgat aggccttgtc aaaggctgca cggacttctt catttccgat ggcgatatcc
      901 agtctgacgc tggatttggg ctgcttgtgc atttctgcag tgatggacac gagcttgact
      961 ccgtatatga tggatgaatt acaaaaaccg ggaatactgg ctttttgcaa agataatacc
     1021 gaccaaccgg gaatacaaat tcaggggctt cggccacgcc ctggcagtcc cgggattgtt
     1081 tccaaaactg tttcttttcc aataaaacgg gtctatcctg agcaaagaag aagcctgcgc
     1141 cattttggtg aggacgcacc cgccggcacc attgcgcagg gaaaggcagg cacaagtaat
     1201 gagtcacaac gcagacatcc aaccactcga gatcgtgtcc cgccagattg atgccgtcaa
     1261 cgagatcctg cgtcttccgg acgacgaaat cgccatcctc aagaaaccgc acaggacgct
     1321 cgaagtcaat ttccccgttg tgatggacga cggacatatc cgtgtgttta ccggattccg
     1381 cgtgcaacac tcccttgtcc ggggaccggc caagggtggt atccgctact cgccggatgt
     1441 cactcgtgaa gaggtccagg ctctggcaac atggatgacg ttcaaatgtg ccgttgtcaa
     1501 tcttccctat ggtggcgcca agggcggcgt gatcgtcgat gtgaccaaga tctccgagcg
     1561 tgaattggaa cgattgaccc ggcgttttac ttccgaaatc gggatcatca tcggaccgga
     1621 cagcgatatc ccggccccgg acatgaatac caacgccaag atcatgggct ggatgatgga
     1681 tacctactcg atgaacaagg gccactccat cccgggggtt gtcaccggaa aaccgattga
     1741 gctcgacggc tccctgggca gggctgccgc gacggggcgc ggggtctcga tcgttctcaa
     1801 cgagttcatg aaactgaaga gtcttgattt gaccaaaatg cgggtcgccg ttcagggata
     1861 cgggaatgtc gggcgctggg ccgcaaaatt gatcgccgca ctgggagcca aagtcgttgc
     1921 catctcggat atcaagggcg ggattcacaa cccggacggg cttgatctgg ccgatgttga
     1981 aaactggacc gacacgcata aattcctcga aggctatccc aaggcccgcg cgatttccaa
     2041 tgcggagctc ttggccaccg attgcgatat cctggttccg gccgcaaccg aggccgtgat
     2101 caaccgcgag aatgcccaca ctgtccaggc ccggtacatc attgaagggg ccaatggccc
     2161 gaccacaccg gatgcggaga atattctggt caaaagcggg acgacgatca ttcccgatat
     2221 cctggcaaat gccggcggtg tcatcgtgtc ctacttcgag tgggtgcaga atatcatgac
     2281 cttcttctgg gacgaggatg acatcaatgg gaaactgatg caggcgctcc agcgggcctt
     2341 caaggatgtt gaaacgatcc acgagcagta tcaggggatc acctatcggc aggcggccta
     2401 tgttctggcg ctgtcccgtg agacaaaggc ccgaagaatg cgcggtatct atccgtaacc
     2461 acaatccatt ccctcctgca ccttcgggtg cgggagggaa tgctgttgtg ccagcatgac
     2521 tgccactgac gggatgggga acgttcttgc ttgtttgtgg gccatagcaa aacagaaagc
     2581 agcaggactc tccaacgtat tcccgttgtg tgtgaggttt gccggagggc cggccgttgt
     2641 ggttcgtacc acccaagcca ttgccttgac aggagaggcc ctgcccgcta cactctgatt
     2701 cggatgttat ataactatgg aggaaatatg ggcaagctcg gaaaagaaat cgtcaaactt
     2761 gacgtcgata ttcttctgaa aaaactcaat tcggcactgg ctgatgaatg gctggcgtac
     2821 taccaatact ggatcggcgc caaggtcgtc aaaggcccga tgaaggacgc tgtcatctcg
     2881 gaactcgata tccacgcaac cgaagaactc ggtcatgcaa ccctgattgc ggcccgcatc
     2941 gtgcagctcg gtggaacacc ggtcctttca cctgacgagt gggccaaggt cgccggttgc
     3001 ggatatgccg ctccggtcga tccctatgtc gccaccgttc tcgatcagaa catcaagggc
     3061 gaacagtgtg caatccggac atacagcgaa ctgctcgatt ttacgcggga caaggatccg
     3121 gtcacctaca atatggttct cggtatcctg tccgatgaag tcgaacacga agaggatctg
     3181 gcctccctgg cggaagacct cgagatgatg cttgaacacc ggaaatgacg tgatcgggtc
     3241 tgtcgggccg ccctgccggg gcggcttttt tttatgagaa aacgattccc acagggcaat
     3301 gatcgctccc catgacagac tggagaatga aggattcgtt gacccggggg gcaagttcct
     3361 tgctgaccac gacgtagtcg atccgccacc cgatgttttt ctttctggcg ttgaaccggt
     3421 aggaccacca ggagtactgg tccggctccg gatgctgtgc gcggaaggta tccacgtagc
     3481 cggcctcgag catcccggta aaccagttgc gctcatcgat gtagaatccc ggattcatcg
     3541 tgttcgattt tgggttggcc aggtcgatct cctcgtgggc gacattcaga tcgccacaga
     3601 aaatcaccgg cttttttttc cggcgtttct cgcaaaacgc caaaaacgcc cgattgtagg
     3661 ccatcttgta gtccagtcgc agcagttcgt gttgcgaatt cgggaaatac gccgcgacaa
     3721 cgaagtattc cggatactct gcggtcagtg tgcgaccttc atcgtcgaat tgcgggtcgc
     3781 ccagaccttc ctgccaggaa aggggcatct ctttgctcag gatgcccacc ccgctatagc
     3841 ccttcttttt ggcaccgacc cagtgtgcgt gatacccgtc cggaaccgca aatgaggtat
     3901 cggctaactc cgggccgagc cgtgtctcct gcaggcagag gatatcgggt ttgtactcgt
     3961 ggaaaaaatc aaaaaagccc ttgtcctgaa cagcgcgcaa accattgacg ttccaggata
     4021 tcagtttcac agatcggcct ccgggtggac agtgtgcttt ctttaagata gcatggtttg
     4081 ggtccggaaa accggtcggc aatgcatggg cagtgcggtg acggtcgacc ttcctgtcga
     4141 aatattcccg gcgtcttctg tggcgggaca ggttgagggc tggttgagag aaatcctctg
     4201 gtgattctgg catgattgcc aacagattgt ccggccgttg cccgggggtg tttgccagga
     4261 ttagtgggga ggtttcggca ggagaggcta aaaaagagtg tcttgtggcc tcgctggaga
     4321 tgaaaaacgc ggtggatagg tgattgacag cgtcggtcac tcgccatatc atgggatacg
     4381 gtgtcgcaaa cgcaattttt gatgcaagca gcgcagcagt ataccgatga tggaagtggg
     4441 acggtttttg tgtgcccgtc gttctttgcc cagcgctgat gaggagaatg cacgatgaat
     4501 ctgcataaag ccaagagttt gagggtcaag ttgacgctct atccggcact gattttcata
     4561 gtagtcggtc ttgttgggac cttggtcaac cttcagattg ccggagacct ttttgaggat
     4621 gtcgggaaag cgtataccca agaaatcctc aaggcaaaac tgagcgaatt cagccagacc
     4681 tttgacagga acatcgacac ctcgcaatac atgtccgaaa acccgattct gcggaactgg
     4741 atgaaaaacg aggatgaccg tggcctcaag gacatggcca tggagaattt ccggaaactg
     4801 cggatcctcc tcaaggatac ggatacgttt gccgcggtcg acacatccaa gagctattac
     4861 gtcaacggga attttctcaa caccctgagc agggacatcg cggctgacct ctggtattgg
     4921 acgtcatcca aaaagcgtga gctggggatc gatatttcct ataacccgca gcttttgacg
     4981 accaaactct gggtctatgt cccggtctgg gatgaggaca ggctcctggg tgtgaccggg
     5041 accggtcttg atgtttccaa gatgatcaag cagatggttt ccaatgtgtc caagggtgcc
     5101 tatgtcattc tcttcaacga cagagggcat gtcaaagcgc acatcaagga agaatacatc
     5161 gacaaccgga cgattttcta tctgacgggt gaggacggga accggattcg cgggatcatg
     5221 aagcggctgg atgaagccaa ggaccaatcc atcatcagcg agttcatcga ttatgacggg
     5281 aagagccata tgggttcgtt ctcgctgatc aatctgcgtt caaccaactg gtacatcctg
     5341 gtgttgatgg acatcaacgc ccttctgtat tcgatcttcg tgccttttat cctcctcctc
     5401 gtgatcagcc tcatgcttat ccttggttcg cttctgatcc tgatcaaccg gatcgtgctc
     5461 catccgctgg gtgtcatcga cagcaatctt gccaaaatca tgaacaagga cttcgatgtc
     5521 aagatcagcc tggccaccgg tgacgaattt acgatggtgg cgaacacgat caacatgatg
     5581 acggccaata tcaaggaata caccgagcac ctcgaagaca tggtcgccca gcggacgagg
     5641 gagttgcagc aggcctttga ggaagtgaat gccctcaagg tgcagcagga cggcgattac
     5701 ttcctgacct ccctgctgat caatcccttg ctggtcaagg aagtccgcag cgagactgtc
     5761 tcggtcgatt tccacatcag ccagaaaaag aagtttacgt tcaaaaagcg caatggtgag
     5821 atcggtggag atgtctgtat cgcccgggag attgtcctga tggacaagcg ttatctggcg
     5881 tttgccaacg gggatgccat gggcaagtcc ttgcagggcg ccggcggtgc gcttgtgatg
     5941 ggggtcgtgt tcaacgcgta tatcaaccgt acgccgctct ttgcgtcgaa caagacgccg
     6001 gaggcatggc tgaccgaaat ctatgaagaa ttgaaccggg tctttgtttc tttcgacggg
     6061 agcatgctga tctccgctgt gatcgggttg cttgatgaag agacgggagc catgtattac
     6121 ctgaatgcgg agcacccctg gacggttttg tatcggggcg gaaatgccgc ctttaccgag
     6181 gaagagctga cactccgcaa aatcggtact gtcggtttcg agaacaaggt tgtcattcac
     6241 atgcagcagc tcgaacaggg ggatgtgatc tacctcggct ctgacggtcg cgatgacgtt
     6301 ttgatcggac acgacgagga gtccgggcag cgcatcatca acgaggatga gacccggttc
     6361 ctgcgctgta tcgaggaatc aaacgggaac cttgagggtg ttgtttcagc cgtcgagagg
     6421 agcgcggagc tgacggatga ctttaccctg atccggatcg agtggaagaa ggccccgctt
     6481 ctcgttcctc cggactacga ggcggtccgc tc
//
LOCUS       DAOSHE010000073         6489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10410_length_6489_cov_4.920267, whole genome shotgun sequence.
ACCESSION   DAOSHE010000073 DAOSHE010000000
VERSION     DAOSHE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_6489_cov_4.920267"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="rpoA"
                     /locus_tag="PLD82_03240"
     CDS             <1..698
                     /gene="rpoA"
                     /locus_tag="PLD82_03240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG64436.1"
                     /translation="IEKKGAGVFKAGDLAVDNGIFVANPDLVIAHLDDDANFTMDLQI
                     EVGRGYMSAEKVGEEIIETVGAIPIDAIYSPIKNVAFTVKNTRVGQRADFDQIALKIE
                     TDGTVKPDDALAQAAKIVKEHLTCFINFDEETEFDTGDGDEAEERLRKILGTPVEELE
                     LSVRSSNCLKMASIQTIGDLVQRSEEDMVKTKNFGKKSLAEIQNKLKSYGLGLGMKDI
                     SLKQMKSQIGKKQ"
     gene            718..1143
                     /gene="rplQ"
                     /locus_tag="PLD82_03245"
     CDS             718..1143
                     /gene="rplQ"
                     /locus_tag="PLD82_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG64437.1"
                     /translation="MRHGKTIAKLGRTSSHRKALMKNLATNLLKREKITTTIDKAKAI
                     RPYVEKLITRAKESSVHNKREAAKKIQDRTVLIKLFDDIGPRFKERNGGYTRIMRLGL
                     RPGDAAEMAIIELVVQSQKEKKKSDKKKKAEKEEAPAAT"
     gene            1204..1857
                     /locus_tag="PLD82_03250"
     CDS             1204..1857
                     /locus_tag="PLD82_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64438.1"
                     /translation="MHGEKNVRRAIERYPKAALILSLLIECLGLALIFSDGFVFFSAA
                     WILLDAFAGGQKPVALAKMICVILPVACMTAIITALSTPDGRLVLDGFVTAEGLELGF
                     LTGLRLVMMALFARTCLLRFGIGPVLVQLSWIMFPLKLAQIGPRLASRTVLRSLVMIP
                     QYVSQAFSILGIQTRRKGLPSVQQIKRLDSRRKILVTIVSLSIGCVLLVFKMSDALQ"
     gene            1992..3584
                     /locus_tag="PLD82_03255"
     CDS             1992..3584
                     /locus_tag="PLD82_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64439.1"
                     /translation="MAKDWQKKLETARKTPGMVQAMLARLPAPEARALDLLYHNGPVQ
                     PRSWLGSKMQISRDELDLLLNRLVALGIVLLSRNRSRLDDSADRIELHSDIRTSIQSE
                     NLVVAVQKKLSILYPQWDGAGPRHLATEYALSCAGVLPLGLDPENLMVPPDTTCFYQS
                     MLLMDQGYVPVHVLREDPESDSDQENRGGRDRLDGIAVLAKIIRHTMRVRMRACEGVM
                     PGKREITAWAESCGYQGETVRMLFSWLAMNGWLMDDQGTLNPARQARTWLASGLKERI
                     DTIREMARARIFKNVPGRKSIKWENLHQAAVQPRQLSSEQDWIEACLATRKALDEVTI
                     FWLLGALSVQLNAGALHKIDLAELDNALGHKPGPLVVTGDMTIVIPEIEASPHQILLF
                     SAFCRVERDSGMIRAKVTEKNFLDGLAAGFDGLEFLRMLEACSRQALPQNIVFSITDW
                     IENTIQAVLHIKPVLHMEENRLDELMHDPAFVKLVDRRVGPNDIILDETDEREIGTIL
                     QRHGIILDCIPDNRHSGDGMRA"
     gene            3490..4803
                     /locus_tag="PLD82_03260"
     CDS             3490..4803
                     /locus_tag="PLD82_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64440.1"
                     /translation="MNEKSEPYFRGTGLSSIVSLTIAILVTGCGLKDVEYYHAAGVKA
                     EMAGKTGDAVTAYGEALKLLPEHYPSLFNLGNMHYLGKNWKEARKNYVKAVIEKTNSA
                     EAWCNLGLTYVQLKERERAYACFLNAWRHDTSNAMPLLAHAASLVDEGKTGEAITILE
                     KGRKSKDSRVFFNLGMLLYDSGEYPKAALAFSSAFEARTNDLKIAVMRVRTSIKLGKE
                     SDAKKHMVAVRALDASGLQSALLQAELDFLGGQLDQADKAVHEHLDLFPDSQAGLLLS
                     GEIAEKRGDLELALTRFTRAAQVDYRSKEAALRAVDVLFRLKRIDLARDRLSILVRSF
                     PEDTRILDGLMRVYFQQGYYDKAAQFGERRLSKGKKSEDAEVITGLSHLMNEDISKRR
                     VDRGIELLWPHRKTQASNRLLLRYLYGALVEKKDTKRAAEIRGML"
     gene            4936..5922
                     /locus_tag="PLD82_03265"
     CDS             4936..5922
                     /locus_tag="PLD82_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64441.1"
                     /translation="MLEIRVNGQPLETQLEEEKTLNDVIRSVETWLLAQDLVITGLRV
                     DGTHVDPADRLFLMQRPVSSAKIMEVTSASLADLEGESSIEIMRYLARLETALGNDDA
                     DIFGNDAREGIRWVCGSLRLLAGIHHLDLGIIPSPEKSILATIQNLEKHVTALDADPE
                     NATIMTAYKDILATSVKTLLVDAYDFLLLVNKRIDDDGNIPAQADHLARAFAEHVQEV
                     STISADLQTGNEARAMMRIQKTVVLIEELMRLFERAGRRYRIGMDSIKLGEESLQFWM
                     GRMMETGNSIINAFHDRDTVLLGDLFEYEISQELEKVPGFLSAVKQSCGSGS"
     gene            5919..>6489
                     /locus_tag="PLD82_03270"
     CDS             5919..>6489
                     /locus_tag="PLD82_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64442.1"
                     /translation="MTGKTGTDGKILEPEELVRLFYRYPINGFPVLDDEYRFSGIVQK
                     RYIINSLHEFRANRMDTRRIIEKHVYCPSEGDIVRMIFGDRKIQPFPVLHRHGRFLGE
                     WQLSRFFQVFDSGDMTGLVEYEQIFEALPGRIVVCDERSYVVMATAAFTHAIAPVIPG
                     RRLRSSLDQGGWQCKEEGESVILERGKKQG"
BASE COUNT         1594 a         1594 c         1800 g         1501 t
ORIGIN      
        1 cgattgagaa aaagggagcc ggtgttttca aggctgggga tcttgctgtt gacaacggta
       61 tctttgtcgc aaaccccgat cttgtgattg cccatcttga tgatgatgcg aatttcacca
      121 tggatctcca aattgaagtc ggtcgcggat atatgagtgc ggagaaggtt ggcgaggaga
      181 tcatcgaaac agttggagcg atcccgatcg atgccatcta ttcaccgatc aagaacgttg
      241 ctttcaccgt gaagaatacc cgtgtcggcc aacgtgctga ttttgaccag attgcactca
      301 agatcgaaac cgatggaacg gtcaaaccgg atgatgcttt ggctcaggct gctaagattg
      361 tcaaagagca tctgacatgc ttcatcaact ttgatgagga gaccgagttt gatacaggtg
      421 acggggacga agccgaagag agactgagga agatcctcgg gactcccgtt gaggaactcg
      481 agctttcggt acgttcttcg aattgcctga agatggcaag cattcagaca atcggtgatc
      541 tcgttcaacg gtccgaagaa gatatggtca agacaaagaa ttttggtaag aagtcactcg
      601 ctgagataca aaacaagctc aagagttatg gacttggtct gggaatgaaa gacatcagtc
      661 tcaagcagat gaaatcccag attggcaaga aacaatgatg aagcaggtaa atacgaaatg
      721 agacacggta agacgatagc caagctcggt cgaacatcat cacaccgcaa ggcactcatg
      781 aagaatcttg cgacaaacct cctcaagagg gaaaagatta cgaccaccat tgacaaggca
      841 aaggcgattc gcccctatgt cgagaagctg attaccaggg cgaaggaaag ctcggttcac
      901 aacaagcgcg aagccgccaa gaagattcag gatcgcactg ttctgatcaa gctctttgac
      961 gacatcggac cgcgattcaa ggagcgtaac ggcggatata ccagaatcat gcgcctcgga
     1021 ttgagacccg gtgatgctgc cgaaatggca attatcgagc tggttgtcca gtcccagaaa
     1081 gaaaagaaga agtcggacaa aaagaagaag gctgaaaagg aagaagctcc agcagctacc
     1141 tgatgattat tcttcttctc gaaaaaaggc tgccgcagtg gcagcctttt tttaggttga
     1201 ttcatgcacg gtgaaaaaaa tgtccggaga gcgatcgagc ggtatccaaa agcggcactg
     1261 atcctttcct tgttgattga atgcctgggg ttggccctga ttttttcgga cgggtttgtt
     1321 tttttctcgg ctgcctggat tcttctggat gcctttgcag gcgggcagaa acctgtggca
     1381 ttggccaaaa tgatttgtgt cattcttcct gttgcctgta tgaccgccat tatcacggca
     1441 ttgagtacgc ccgatggcag actggttttg gacgggtttg tgaccgctga aggccttgag
     1501 cttggttttc tgacagggct tcgtctggtc atgatggcac tttttgcacg gacgtgcctg
     1561 cttcgcttcg gaatcggacc ggttctggtg caactctctt ggattatgtt tccactcaaa
     1621 cttgcccaaa tcggtccgcg actggcctca aggactgtgc ttcgaagtct tgtcatgatt
     1681 ccgcaatatg tctcgcaagc gttttccatc ctggggatac agacaagacg aaaaggtctg
     1741 ccttctgttc aacaaataaa aagactggac agccgacgga agatattggt caccatcgtc
     1801 agtttgtcga taggctgtgt gctgcttgtc ttcaagatgt ccgacgccct ccaatgacaa
     1861 atgttggcag agaatccgtc cagggagaat cttcgtgcag aatgaacaag agcatgctat
     1921 aattgacggc aatgcagaaa aagacagcaa taaaagatcc ttcctgcctt gaattgtggg
     1981 ggattcccct tttggcaaag gattggcaga agaaactgga aacagcacgg aagacgcccg
     2041 ggatggtcca ggccatgctc gccaggcttc cggctcccga agccagggca ttggaccttc
     2101 tgtaccacaa cggtccggtc cagccacgct cctggctcgg gtcaaagatg cagatttcga
     2161 gggacgaact tgaccttctc ctcaaccgtc tcgtcgcatt ggggattgtt ctcctttcaa
     2221 gaaaccggtc gcgtcttgat gattctgcag accggatcga acttcacagc gatatccgca
     2281 cgtccatcca atcggaaaat cttgtcgtgg cagtacaaaa aaaactctcg atcctctatc
     2341 cacagtggga tggtgcaggg ccgcggcatc ttgccacgga gtatgcgcta tcatgcgcgg
     2401 gtgtgctccc actcggtctt gatccggaga atctcatggt cccgccggat acgacatgtt
     2461 tttatcagtc catgctcttg atggatcaag ggtatgttcc tgtccatgtt ttgcgtgagg
     2521 atccggaatc agattctgat caggagaatc gcggtgggcg agaccgcctg gacgggattg
     2581 ccgtccttgc gaaaatcatc cgtcacacga tgcgtgtccg gatgcgggcc tgcgaagggg
     2641 tcatgccggg caagagagag atcactgcct gggccgaatc ctgcgggtat caaggcgaaa
     2701 cagtccgtat gctcttttcg tggttggcca tgaacggttg gctcatggat gatcaaggga
     2761 cgctcaatcc ggccagacag gcccgcacat ggcttgcctc gggattgaag gagcggatcg
     2821 acacgatccg tgagatggcc cgtgcccgga ttttcaaaaa tgttccaggc cgaaagagca
     2881 tcaaatggga gaatctgcac caggcggctg tacaaccccg gcagttatcc tccgagcagg
     2941 actggattga agcctgtctc gcgacgagaa aagcccttga tgaagtcacg atcttctggt
     3001 tgctgggtgc tctttctgta caattgaatg cgggggctct ccacaagatt gatcttgccg
     3061 agctggacaa tgctcttgga cacaagccgg gtcccctggt tgtgacggga gacatgacga
     3121 ttgttattcc cgaaattgaa gcttctccgc accagatcct gctcttctcg gcattctgca
     3181 gggtcgagcg tgacagtggc atgatccggg ccaaggttac cgagaaaaac ttcctggatg
     3241 gtcttgcagc cgggtttgac gggctggaat tcctccgcat gctggaagcc tgctcccggc
     3301 aggctttgcc acaaaatatc gttttttcga tcaccgactg gattgaaaac accatacagg
     3361 cagtattgca tatcaagcct gtcttgcaca tggaagagaa caggctcgat gaattgatgc
     3421 atgatccggc cttcgtcaag ctggttgacc gccgggtcgg gccgaatgat attattctgg
     3481 atgagacaga tgaacgagaa atcggaacca tacttcagag gcacgggatt atcctcgatt
     3541 gtatccctga caatcgccat tctggtgacg ggatgcgggc ttaaggacgt cgagtattat
     3601 catgctgcgg gcgtcaaggc cgagatggcg ggaaagacgg gcgatgctgt cacggcctat
     3661 ggtgaggccc tcaaactctt gccggagcat tatccatcgc tcttcaatct gggaaacatg
     3721 cattatctgg ggaaaaactg gaaagaagcc agaaaaaatt atgtcaaggc agtcatcgaa
     3781 aaaaccaatt cggcggaagc ctggtgcaac cttgggttga catatgtcca actcaaggaa
     3841 cgcgaacgag cctatgcatg tttcctgaac gcatggaggc atgatacgag caatgccatg
     3901 ccgctcttgg cccacgctgc ctctctggtt gatgaaggca agacgggtga ggccatcaca
     3961 attcttgaaa agggaagaaa gagcaaagac tccagggtct ttttcaatct ggggatgctc
     4021 ttgtatgatt ctggcgagta tccaaaagcc gcactggcct tttcttccgc cttcgaggca
     4081 cgcaccaatg atctcaaaat tgccgtgatg cgggtacgca cgagtatcaa gctgggcaaa
     4141 gaatccgatg caaaaaaaca catggttgcc gtccgtgcac tggatgccag cggcttgcag
     4201 agcgcgcttc tccaggctga actggatttt cttggggggc agcttgacca ggcggacaaa
     4261 gctgtccatg agcatcttga tctctttccc gacagtcagg ccggcctcct gctttccgga
     4321 gagattgctg aaaagcgtgg cgacctcgag ctggcgttga cacggtttac cagggccgcc
     4381 caggtcgatt accgcagcaa agaagcggcg cttcgggctg ttgatgtcct tttcagactc
     4441 aaacggatcg accttgcccg ggaccgcctg agcatcctgg tccgttcgtt tcccgaagat
     4501 accagaatcc tcgatggcct gatgcgggtc tatttccagc aaggatatta tgacaaagcg
     4561 gcccaattcg gagagcgacg tttgagcaag ggcaagaagt ccgaggatgc agaggtcatt
     4621 acggggttgt cacacctcat gaatgaggat atctcgaagc gtcgggtgga ccgagggatc
     4681 gaacttcttt ggcctcaccg caagacacag gcttcaaacc ggcttctcct gcgctatttg
     4741 tacggagcgc tggttgaaaa aaaagatacc aagcgcgctg ccgagatccg cgggatgctc
     4801 tgatctggcg aagtgcggat ttcccgcgcg gcaccagtcc cttcagcgag aatcgtgtct
     4861 tccatccttc agcctcttcg atattttgcc gacaagacag gttgtgtggc ttcgatgcat
     4921 gccgggagga tgatgatgct tgaaattcgg gtgaatgggc agccccttga aacgcagctt
     4981 gaagaagaaa agactttgaa tgatgtgatc agatccgtcg agacatggct tcttgcccag
     5041 gatctggtta tcaccggact gagggttgac gggacacatg tcgatccggc cgaccgtctc
     5101 tttctcatgc aacgtcccgt ctcttcagcc aagattatgg aagtgacgtc ggcatccttg
     5161 gccgatctcg agggcgagag cagcatcgaa atcatgcggt acctcgccag actggaaacg
     5221 gctttgggga atgatgatgc cgacatcttc gggaacgacg cccgcgaggg cattcgctgg
     5281 gtctgtggaa gtctcagact ccttgcaggg atccatcatc tggatctggg catcattcca
     5341 tctcccgaaa aatccattct ggcaaccata caaaatctcg aaaaacatgt gacggcactc
     5401 gatgcggatc ccgaaaatgc cacgattatg acggcttaca aagacattct cgccacgtcg
     5461 gtcaagacac tgcttgttga cgcctatgat tttctccttc tggtcaacaa aaggatcgat
     5521 gatgacggca atatcccggc ccaggctgac catctcgccc gggcttttgc cgagcacgtc
     5581 caggaggttt ccaccatttc tgcagatctt cagacgggaa atgaagccag ggccatgatg
     5641 cggatccaga aaacagtcgt cctgatcgaa gagctgatgc gactctttga acgtgccggc
     5701 cggcggtacc ggatcggcat ggattcgatc aagcttgggg aagagtccct tcagttctgg
     5761 atgggcagga tgatggaaac cggaaacagc atcatcaatg ccttccacga cagggataca
     5821 gtcctgcttg gtgacctgtt cgagtatgaa atcagtcagg aacttgaaaa ggtccccggg
     5881 tttctctctg ccgtgaaaca gtcctgtggc agcgggtcat gaccggaaaa accggaactg
     5941 acgggaagat tcttgagccg gaggaactgg tgcggctttt ttaccgatac ccgatcaacg
     6001 ggtttccggt cctggatgac gaatatcggt tcagcgggat tgttcaaaaa cgatatatta
     6061 taaacagtct tcatgaattc cgcgcaaacc ggatggatac ccgccgcatc atcgagaagc
     6121 atgtctattg tccctccgaa ggtgatatcg tccggatgat ttttggtgac cggaagatac
     6181 agccatttcc cgtcctgcac cgacacggac gttttttggg cgagtggcag ctctcacgat
     6241 ttttccaggt ctttgacagt ggtgacatga cgggactggt cgaatacgag cagatttttg
     6301 aagcgcttcc gggccggatc gttgtctgtg atgaacggag ttatgtggtc atggcgacgg
     6361 ccgcttttac gcacgcgatc gcgccggtca ttcccggacg gcgtctgcgc tcttcactgg
     6421 atcagggtgg atggcaatgc aaagaagagg gtgaaagtgt cattctcgaa agaggaaaaa
     6481 aacagggct
//
LOCUS       DAOSHE010000074         6379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10689_length_6379_cov_4.274352, whole genome shotgun sequence.
ACCESSION   DAOSHE010000074 DAOSHE010000000
VERSION     DAOSHE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10689_length_6379_cov_4.274352"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..512
                     /locus_tag="PLD82_03275"
     CDS             108..512
                     /locus_tag="PLD82_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64443.1"
                     /translation="MSMMVTIKSVIKSDTILEECLALLDIELVVHQRDELGRISQAEI
                     LYPAHIRSVTESPVFCTVSRTADGYLELQRDEKLNTKDVRKHLSEILDLREEIIKGCA
                     SLATVQALEQQGYTILRKEAVANTIVIEACIA"
     gene            525..728
                     /locus_tag="PLD82_03280"
     CDS             525..728
                     /locus_tag="PLD82_03280"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOG64444.1"
                     /translation="MEKITVTINSYGVAKVHIEGIHGPRCSETVEHLSSHLGPVSLRT
                     ETSEYYEQEITTQVDQHTRVTPH"
     gene            742..2346
                     /locus_tag="PLD82_03285"
     CDS             742..2346
                     /locus_tag="PLD82_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG64445.1"
                     /translation="MNGIQRLTTLVKARTPAILVVGHEEERYLDLLHGMGVPVLYWTM
                     SLGLGVWQTSDSIFREPQKDAPSNPVQSPMAMPALDVLKHIISPKAPSGIYVLCDLIR
                     LTVSNPPLIRMLRDAIQHLRRHPGRTLVITSTTEVVPPELEKEIATVHMPLPDSRLVS
                     DHLAQVIKRVALERPDMVGDGFDRNAVEAALSGLTLNEMDNALAASIAGQETISADAV
                     LEEKKAQLVSNGLLEIAAIPEEIKGLASAGGLEYLKDWLAVRRPVFLHDGSSHAVIDV
                     RQHRIPVPKGILLLGVQGCGKSHFVRCLAREWGLPLVNLSMSRIFASLVGQSEQQMRQ
                     ALEIVDTMAPVILFVDELEKAFGGVASSSTTDGGTTARVTGTFLSWMQDRTSRVFVTG
                     TANDIASLPLELVRKGRFDEVFFVGLPHARERAAIIDIHLAQHGLDALVGQCPALVEA
                     TEGYSGAEIAQLVIDTLYEHFCSQQPVDEASLIVRARETKPLSETRSQAIEALTEWAE
                     GRTVSASRCDHSASVIRFAPPGGRQT"
     gene            2359..2922
                     /locus_tag="PLD82_03290"
     CDS             2359..2922
                     /locus_tag="PLD82_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOG64446.1"
                     /translation="MLSIHAILTHSRVNGPGDCCVVWLQGCIHDCPGCCNTAACSRTR
                     GEILLAAEHLADDVIAARPDCLSLSGGEPFLQARELVSVARRVHAAGIGVLAWSGYEY
                     EALRDSRNPWQRELLEELDILVCGPYLRNVPQREIWAGSGNQRTLFFGTRFADWEPRI
                     AGKGHIECTIRGDGSGILTGFGSFSNA"
     gene            complement(3015..4151)
                     /locus_tag="PLD82_03295"
     CDS             complement(3015..4151)
                     /locus_tag="PLD82_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HOG64447.1"
                     /translation="MPVTFINHYHDTNPVPLDPARIPLALSLEPVESGFFRRRQAWLV
                     AAGRRLARVRWKRDAYHNLFLDILETAPGYTPAQAALDQEVTIQLVSAAWDVRHEDLE
                     RIAPGSVLCSGQLVTAELVLTIPEWECTVGRAIYAHDANGRKFAQIVRMETGYPLHAD
                     RPARPAGADGTHQVRIIFGRTRLPVRELLSLGNTSSLRLDGLVSHPVRVTVDGEERFE
                     GIVREQRGWIAVQISGSAGETSPELQALQDEIFPGMIEYKIFENSLNSLGESMVKNRQ
                     YPEDIVQRFSALLAEIPDDEMTAMLDEVHPELGVIIASHLPTERFARIIRLMNKEQAI
                     KVGTGFAHKHTILAHFVGRIHDWVCTKRRENSQETSIRRKNALL"
     gene            complement(4164..5096)
                     /locus_tag="PLD82_03300"
     CDS             complement(4164..5096)
                     /locus_tag="PLD82_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64448.1"
                     /translation="MADPVEEYLESIRSERTNDVQTRQPRSRSSENSISQEEIDKLLE
                     IMEERIRQDGIHRGMQDSLVEPLTEYDYANPAILSPYQRRLMELAMGRWSALFQHSCR
                     RLFKSAIYDVVFEPIRIFKQEWFEKHIMKYDYIVFLKTEAGSGEAALLIREAVMRQIF
                     INAMKGSCFNQDNDSYFLLTEINMAIVRDFFTVVVTDLERALYGLDCEIRDGIIIDQI
                     NSQSCTFFSDYPALHIPFMMKFGFYDFQMELLLSQAFLENWKQSNRFPEPVRRPDRKH
                     ERILPVPVPVVAGSLDLVPGCRIILPVRPGDRRH"
     gene            5211..6206
                     /locus_tag="PLD82_03305"
     CDS             5211..6206
                     /locus_tag="PLD82_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64449.1"
                     /translation="MSKPICRGSIPFFTRYGSRADYGVIHVFRTADRYLLVARLGDTT
                     VCSAEYTTRDGTHVPDLGSSHVPQPLLSRLAGLVWHDIAQHLQDSGYSGVFPFALYDW
                     SPDNRWDVLTERLPDKDITLTNAALIRLAEDCARDPYYRWFGAYLMKQTDWINTCSPA
                     ERERLEEVVRRIPLLIPALPSMYLQKIPSSDQLWQTSVMQRIVTGRTDWSLEWAYHEV
                     LYAWNSNIVHDIKYLKKYCTSEEIPENTAGTACVIIHEILVMLDKIFSQPPVKLQSLL
                     AAFDQIHRLRVFGYGKPKEAAVNAMQKRLDAIIANLACRAVSKTPGFTPTCEDPS"
BASE COUNT         1408 a         1905 c         1741 g         1325 t
ORIGIN      
        1 cggtcagccc acgcatcgcc cccggtatcc aacctttttt atccagcctg caatttttct
       61 ggcgtcccgc tcgtatctcc cggtatcacc aacccgggag gcagcacatg agcatgatgg
      121 tcaccatcaa atcggtcatc aagtcggaca ctattctcga ggaatgtctc gcactgctgg
      181 acattgagtt ggtcgtccac caacgggacg aactgggccg gatcagccag gccgagatcc
      241 tctaccccgc ccatatccgg tctgtgaccg agtcacccgt cttttgcact gtcagccgca
      301 cagcagacgg ctatcttgag cttcagcggg acgaaaaact caacacgaaa gatgttcgga
      361 aacacttgtc cgaaattctc gatctcaggg aagaaatcat caagggctgc gccagccttg
      421 cgaccgtgca agccctcgaa caacagggct ataccatcct gcgcaaggaa gccgtcgcca
      481 acacgatcgt catcgaagcg tgcatcgcct gaggaggctt gtctatggaa aaaatcacag
      541 tcaccatcaa cagttatggt gtggccaagg tccacatcga aggcatccac gggccacgct
      601 gcagcgagac tgtcgaacac ttgagcagtc atcttggtcc ggtcagcctt cgcacggaga
      661 cctcggagta ttacgagcag gaaatcacca cccaagtcga tcagcacacc agagtcacac
      721 cacactgaca gggaggttgg catgaacggt atccagcgac tgacaaccct ggtcaaggca
      781 cggacccctg ccatactcgt tgtcgggcat gaggaagaac gctatctcga tctgctgcac
      841 gggatgggtg taccggtcct gtactggacg atgagtctgg ggcttggagt ctggcaaacc
      901 tccgactcca tcttcaggga gcctcagaaa gatgcgccat ccaatcccgt gcaaagcccg
      961 atggccatgc ctgcactgga tgttctcaag cacattattt cgcccaaagc cccctccggc
     1021 atctatgtgc tgtgcgactt gatccggttg accgtttcaa atccgcccct gatccgcatg
     1081 ctgcgcgatg cgatccagca tctgcggcgg caccccggca ggacccttgt catcacttcg
     1141 acgaccgagg ttgttccccc cgaactcgaa aaggaaatcg ccaccgtcca catgcccctg
     1201 cccgactccc gactggtcag cgaccatctg gcacaggtca tcaagcgtgt cgctctcgaa
     1261 cgccccgaca tggtcggcga cggttttgac cggaacgccg tcgaggcggc actctccggt
     1321 ctgaccttga acgagatgga caatgccctc gccgcctcaa tcgccggaca ggagaccatc
     1381 agcgcggacg ccgtcctcga ggaaaaaaag gcccagctcg tctccaatgg cctcctcgag
     1441 attgccgcca tccccgagga aatcaagggg ctggcatcag ccgggggact cgaatacctc
     1501 aaggactggc tggccgtccg ccggcccgtc tttttgcacg acgggtccag ccatgccgtg
     1561 atcgatgtcc gccaacacag gattccggtt cccaaaggga tcctgctcct cggagtccag
     1621 ggctgcggca agagccattt tgtgcgctgt ctggcccggg aatgggggct gcccctcgtc
     1681 aatctctcga tgagccggat cttcgcctcg ctggtcggtc aatccgaaca acagatgcgc
     1741 caggccctcg agatcgtgga taccatggca ccggtgatcc tgtttgtgga cgagctcgaa
     1801 aaagccttcg ggggagtggc cagctcgtcc acgacggatg gaggaaccac ggcccgcgtg
     1861 acaggcacgt ttttaagctg gatgcaggac cggacctcgc gggttttcgt gacggggacc
     1921 gccaatgaca tcgcctccct gcccctcgaa ctggtccgca agggacgctt tgacgaggtc
     1981 ttttttgtcg gtctcccgca cgcccgcgag cgcgccgcga tcatcgacat ccacctggcc
     2041 cagcatggtc tggatgccct tgtcgggcag tgtcccgccc tggtcgaggc caccgaaggc
     2101 tacagtggtg ccgagatcgc ccagcttgtg atcgataccc tctacgagca tttttgcagt
     2161 caacaacccg ttgacgaagc gagcctgatc gtgcgtgccc gcgagaccaa gccgctctcc
     2221 gagacccgct cccaggcgat cgaggccctg accgaatggg ccgaaggccg gaccgtgtcc
     2281 gcctcgcgtt gtgatcacag tgcatccgtc atccgctttg ccccaccggg cgggagacag
     2341 acctgaaagg aggtcatcat gctctcgatc cacgccatcc tcacacactc ccgggtcaac
     2401 ggaccgggag attgctgtgt cgtctggctg caaggctgca tccatgactg tcccgggtgc
     2461 tgcaacacgg ccgcctgcag ccggacgcgg ggagagatcc tcctcgctgc cgaacacctg
     2521 gccgacgacg tgattgccgc ccgccccgac tgtctttcgt tgagcggtgg tgaaccgttt
     2581 ttgcaggccc gggagctggt gagcgtggcc cggcgggtcc atgcggctgg tatcggcgtc
     2641 ctggcctgga gcggatatga atacgaagcc ctgcgggact cgcgcaatcc ctggcagcgc
     2701 gaactccttg aggagctgga catccttgtc tgcggtccct acctccggaa tgttcctcag
     2761 cgcgagatct gggccggctc gggcaaccag cggacactct ttttcggaac ccgctttgcc
     2821 gactgggaac cgcggatcgc cggaaagggg cacatcgagt gcaccatccg cggggacggt
     2881 agcgggatcc tgaccgggtt tggctcgttt tccaatgcct gacaacatac aacagtattt
     2941 tgaaacgctc ttatagcatt cccgatccca gaacattttc gtgatacgcg catgcatccg
     3001 cagagcggtc agtctcagag gagcgcattt ttccttctga tggaggtttc ctggctgttc
     3061 tctctcctct tggtgcacac ccagtcatga atgcgcccca caaaatgggc cagtatcgtg
     3121 tgtttatgtg caaatccggt accgaccttg atagcctgtt ccttgttcat cagtctgatg
     3181 atcctggcaa atcgttctgt cggaagatga ctggcaatga tcacgcccag ctccgggtga
     3241 acctcatcca gcatggccgt catctcgtca tccggaattt cagcaagaag tgctgaaaag
     3301 cgctggacga tatcctcagg atactgccgg tttttaacca tggattcgcc aagagagttg
     3361 agcgaattct caaagatctt gtattcgatc atcccgggga agatctcgtc ctgcagagcc
     3421 tggagttcgg gcgatgtctc tccggccgac ccgctgatct ggacggcaat ccagcccctc
     3481 tgctcgcgga cgatgccctc aaagcgttcc tcgccatcaa ccgtcaccct gaccgggtgc
     3541 gagaccagcc cgtccaggcg cagactgctt gtgttgccaa gggacagaag ttcgcgcacc
     3601 ggcaaacgtg tccgcccgaa gatgatgcgc acctgatgtg tcccgtcagc acccgcaggc
     3661 cgggccggac ggtcagcatg cagcggatag cccgtttcca tccgcacgat ctgggcaaac
     3721 ttgcggccgt ttgcatcatg ggcgtagatg gcccgaccga ccgtacactc ccattcgggg
     3781 atggtgagca cgagctctgc cgtgaccagt tgtccggaac agaggaccga gcccggggcg
     3841 atacgctcga gatcctcatg cctgacgtcc caggcagcgg agacaagctg gatcgtgacc
     3901 tcctgatcaa gtgcggcttg agcgggagta taccccgggg ctgtctcgag gatatccagg
     3961 aaaagattgt ggtacgcatc ccgcttccag cggacccggg ccaggcgccg tccggcagcc
     4021 acaagccagg cctgtcggcg cctgaagaat ccggactcta ccggctcaag ggagagggcc
     4081 agcgggattc gggccggatc gagcgggacc gggtttgtgt cgtgatagtg attgataaag
     4141 gtgacgggca taccagcctc cgattaatgg cgtctgtccc cgggcctgac aggaaggatg
     4201 atccggcagc cgggaacaag atcaagtgaa ccggccacga ccggaacagg aacgggcaag
     4261 atccgttcat gtttccgatc gggcctgcgc accggttcag gaaaccggtt agattgtttc
     4321 caattttcaa gaaaagcctg agacaggagg agctccattt ggaagtcata gaaaccaaat
     4381 ttcatcatga aaggaatatg aagagcaggg tagtcggaaa agaaggtaca agactggctg
     4441 ttgatttggt caatgatgat cccgtccctg atttcacaat caagcccgta caatgccctt
     4501 tcgagatcgg tcacaacaac ggtaaagaag tccctgacaa ttgccatgtt tatttcggtc
     4561 aataaaaaat aactatcgtt atcttggttg aaacaactgc ccttcattgc attgataaag
     4621 atctgtcgca tgacagcctc tctgataagc aaggcagctt ctccactccc ggcttcagtc
     4681 ttcaggaaga ctatgtaatc atatttcatg atatgttttt caaaccattc ctgcttgaag
     4741 atccggattg gttcaaaaac aacatcatag atggccgact tgaataatcg tcggcaacta
     4801 tgttgaaaaa gagctgacca tctacccatg gccaattcca tcagacgccg ctgataggga
     4861 gaaaggatgg cggggttagc gtagtcatac tcggtcaggg gttcgacaag cgaatcttgc
     4921 atccctcgat ggataccatc ttgtcttatt ctctcttcca ttatttcaag gagcttatcg
     4981 atctcttcct gtgaaatgga attttccgac gagcgggacc tgggttgacg ggtctgcacg
     5041 tcattcgttc tttccgacct gatcgactca agatattcct caacgggatc tgccatacgc
     5101 cacctccctt ccgcacaaga gtagtacgta ccggacacga caaaaattgc accccttgat
     5161 tgcaattttt gctggtgtga atccgtatcc tgtgacatgg agggaaaccc atgtccaaac
     5221 caatatgccg gggaagcatt ccgtttttta ccagatatgg cagccgggca gactatggcg
     5281 tcatccatgt cttcaggaca gcggaccgat atctcctcgt ggcaaggctg ggtgatacaa
     5341 cggtgtgcag tgccgagtac acaacccgtg acgggacaca cgtcccggat cttggcagca
     5401 gtcatgtgcc gcagccactg ctctcccggt tggcaggtct tgtttggcac gatatcgcac
     5461 agcatcttca agattccggc tacagcggcg tgtttccgtt cgcactctat gactggtctc
     5521 ccgacaatcg ctgggatgtc ttgacagaac gcctgccgga caaggatatc acgctcacaa
     5581 acgcggcact gatcaggctg gccgaggact gcgcccgtga tccgtattac cgctggtttg
     5641 gtgcctatct catgaagcag acagactgga tcaacacctg cagcccggcc gaacgggaac
     5701 gtcttgaaga agtggtcagg aggatcccgc tcttgatccc agccttgccc agcatgtacc
     5761 tgcaaaaaat cccctcttca gaccaactct ggcagacatc tgtcatgcag aggatcgtca
     5821 caggccggac tgactggagc ctcgaatggg cctaccatga agtactgtac gcatggaatt
     5881 ccaacatagt ccatgatatc aaatatttga agaaatactg cacctcggaa gaaataccgg
     5941 aaaacaccgc agggacagcc tgcgtgatca ttcatgagat actcgtcatg ctcgacaaga
     6001 tcttttcgca accaccggtc aaactgcagt ccctgctggc ggcattcgac cagatccacc
     6061 gtctgcgggt gtttggttac ggcaagccca aggaagcagc ggtcaacgcg atgcaaaaac
     6121 gactggatgc gatcattgcc aatcttgcct gtcgcgctgt ctccaagacc cccggcttca
     6181 ctccaacctg tgaagatcct tcatgaatac tgccgacccg atacgacatt cccccgcccg
     6241 gggaaggccc ccgggcaagg gatgggctga cagacacggc cggaccggca atgcagagta
     6301 tcacgaattt ggcttgagcc cgaacaattg tttgagattc cccttgatga tgcgatagcc
     6361 cagggcaatc gcggccaga
//
LOCUS       DAOSHE010000075         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10725_length_6364_cov_3.950864, whole genome shotgun sequence.
ACCESSION   DAOSHE010000075 DAOSHE010000000
VERSION     DAOSHE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10725_length_6364_cov_3.950864"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..986
                     /locus_tag="PLD82_03310"
     CDS             120..986
                     /locus_tag="PLD82_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG64450.1"
                     /translation="MKSMTGYGVHRQQVGAYSYTVEIKSVNNRYLEIQTRMPHFLSPW
                     DGQIRKSIEAIASRGKVDVWIDVSVQDGAIDAQVDAGLVQAYLKAHELVARMVGDSSN
                     VTAHQIMGYEGVIQIRKTTPPETVWRDLKPCLDAALKKFLAVRVHDGQATRRDLQKLL
                     TVLGRSIQTVRKRAKGLVPDYANELKNRLKAMTGKTIDQNLIMSEAALIASRTDINEE
                     LTRLESHKALFLEHLSGDEPCGKTLDFVAQEMNREINTIGSKQNDLALSNEVIRMKSL
                     LEKIREQARNIE"
     gene            983..1570
                     /gene="gmk"
                     /locus_tag="PLD82_03315"
     CDS             983..1570
                     /gene="gmk"
                     /locus_tag="PLD82_03315"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG64451.1"
                     /translation="MIDQKRLCIISGPSGAGKTSIYKKLLSEFDWLRYSVSVTTRDAR
                     PGEKDGQDYYFLGHERFEQLKNEMAFAEYAQVHGNWYGTLKSELAEKTRPGVLCILDI
                     DVQGAARLRALYPDALKIFVRPPDVATLEKRLMGRSTESPEVRALRVSNAVRELEDEH
                     FFDYSVVNTDFEQAYAQVQEIILQRKGALDNGHSV"
     gene            1554..1781
                     /locus_tag="PLD82_03320"
     CDS             1554..1781
                     /locus_tag="PLD82_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64452.1"
                     /translation="MAIPFEKLLNRNENRYEMCVAAMKISQHIATTTSSEEIGKMRDK
                     LAVLAISKLLDEKIPIKTGVDAASRKVRDDY"
     gene            1797..2699
                     /gene="miaA"
                     /locus_tag="PLD82_03325"
     CDS             1797..2699
                     /gene="miaA"
                     /locus_tag="PLD82_03325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG64453.1"
                     /translation="MTIPMICGPTAAGKTAAACAVFGGKDVLAISLDSMQVYTGMDIG
                     TAKPDPEERSRLEHVMIDCADPRQVWSVGTHLKQTEAWITRARESGRQVVLVGGTGLY
                     VKGLINGLFDGPARDPALRAELEEREKTRPGVLYRELLGRDPETAKRLSQADIRRIIR
                     ALEVLTLTGTGITRLQREQTVRNPLKFLLAGLIVDRDELARRIEERVDRMYGAGLVDE
                     VRDLLDSGCHEALPSMQGVGYRETIAFLRGRISLDEARAQCIVNTRSLAQKQIMLFRR
                     IPELVWFHARDHERLAAYLAGGSG"
     gene            2696..3709
                     /locus_tag="PLD82_03330"
     CDS             2696..3709
                     /locus_tag="PLD82_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64454.1"
                     /translation="MKGGKLFGIIALGYEQRRLWPLDLANRPFACRPFFGGKTLLEAS
                     FERMRVLIPDDRIWVIAPQSLLPLVASLLPGLDSRRLYAGSGTNPGDLYALTETISLI
                     DPDGQVVFSPAGIIAGDDQAFSDLVHDAARLAHATGCIVTGTVPVSGIRAGYPCILPG
                     DACIRSGRVFGRDIRRYFPSVPSADAEAISARGAVWATGIHVWNTATFLVDVLGFLAG
                     QQDMDESDDLSSRFFSQSKNWLMVESALVWDPMRSFEDLARYIRGDGSGNSRSGHCIA
                     GQTRNSIILSDNPGGLVVVHGLHDVLVVQTGETILCCSRGDEEGVNRLLSSLAGIQQE
                     AGS"
     gene            4038..4712
                     /locus_tag="PLD82_03335"
     CDS             4038..4712
                     /locus_tag="PLD82_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOG64455.1"
                     /translation="MFLASRMLGFAALCLCTALPLAGVSLWQDGHLFSSRGKVVPGDI
                     LKIRFSYRNLVRYRTEMKAGDTQKLNLGKPDLKTFSFLPSIDNNTTYNRNNNLEYTSE
                     REFATTIAVMVQTIASNGIISFRGNHSLQLNGQNEEVVIQGQVRSQDVADGNFVASTD
                     IANLNFAWIGPSVIRQNVLRTGDLTVSTNTNQRAGQDAPELTNEVKRRLLMQYLNRIH
                     DILFRP"
     gene            4722..4877
                     /locus_tag="PLD82_03340"
     CDS             4722..4877
                     /locus_tag="PLD82_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64456.1"
                     /translation="MASILIVDDSAFVTTQLTQILVKGGHTVSAALPVETKRSPSSRK
                     TQVPSIL"
     gene            4874..5104
                     /locus_tag="PLD82_03345"
     CDS             4874..5104
                     /locus_tag="PLD82_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64457.1"
                     /translation="MTLDITMPGTSGIDTLRHIRMHWPGILCVVVSGIGKRETVLETR
                     ELGAAGYIVKPLSRERVLSKIGEILSETRVES"
     gene            5170..6126
                     /locus_tag="PLD82_03350"
     CDS             5170..6126
                     /locus_tag="PLD82_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG64458.1"
                     /translation="MRSLFLAFLLSGVFVACSSQKTHDSLDAAIAAAGSNSRDILIIV
                     SKSDECHACSLLEKNILTNPQWKPESLYEVVRIDLTEKLKKEHPELWKSGDTIQTRYG
                     ARWFPAIILTDSRGMPYHIGGYDESSPEEFFKLLEGRTKERRHELAAILAKATNGMKA
                     RAALHKLKEWGVDTAYHKLKALAMGGSRDPGFAIELVEYWTALGDLKKREEWLRVLET
                     GWPQAWRDTLVRLDVEQIEKNEFEKMEWKAARAKLEPLMAKKPLGIAASRLYAALGEV
                     AYQLRERGPCLGYFSQALEHAPEGKLKTMLTNRLAYLKKNSF"
     gene            6140..>6364
                     /locus_tag="PLD82_03355"
     CDS             6140..>6364
                     /locus_tag="PLD82_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG64459.1"
                     /translation="MQSVLAFDIGGTFIKAARVSSTGEILQRVRVPVSPSREEMCAIL
                     RRVAQDLLGDHEPETTGIGVGAPGAIDRSVP"
BASE COUNT         1478 a         1663 c         1817 g         1406 t
ORIGIN      
        1 agcgcttgaa tggcattcaa gaggtcaggg gttcgactcc cctcatctcc acaccggatt
       61 tctttccata atgcagcgcc gctccgaaag ggcggcgttt tgtatttcag ggggcaggga
      121 tgaagagcat gacaggctat ggtgtccatc ggcagcaggt gggtgcgtat tcctatactg
      181 tcgagatcaa gtccgtcaac aacagatacc tcgaaatcca gacaaggatg ccgcactttt
      241 tgtcgccatg ggacgggcag atccggaaat ccatcgaagc gattgccagt cggggcaaag
      301 ttgatgtctg gattgatgtc tccgtccaag atggtgccat cgatgcccag gtcgatgcag
      361 gactggttca ggcctatctc aaggctcacg aactcgtcgc caggatggtc ggggattcaa
      421 gcaacgtcac ggcccaccag atcatgggat acgaaggtgt gatccagatc cggaagacaa
      481 cccctccgga gactgtctgg cgggacctca aaccctgtct tgatgcagcc ctgaaaaaat
      541 ttcttgctgt ccgcgttcat gacgggcagg caacacgccg ggatctgcaa aaactcctga
      601 cagtgctcgg aagatccatc cagacggtcc gaaaacgggc caaagggctt gttcccgact
      661 atgccaacga actcaaaaat cgtctcaagg ccatgacggg aaagacaatc gaccagaacc
      721 tgatcatgag cgaagccgcg ctcattgcaa gccggacgga tatcaatgag gaactgacca
      781 gactggagag tcacaaagcc ttgtttctgg aacacctttc gggagatgag ccctgtggca
      841 agactctgga ttttgtggca caagagatga accgtgaaat caacacgatc ggatccaaac
      901 agaatgatct cgccctctcc aacgaggtca ttcggatgaa gtccctgctc gaaaaaatcc
      961 gggaacaggc gagaaacatc gaatgatcga tcaaaaaagg ctgtgcatca tcagtggtcc
     1021 gtccggtgcc ggaaaaacca gcatctataa aaagctcctt tcggagtttg actggttgcg
     1081 ctattctgtc tcggtgacta cccgggacgc aagaccgggg gagaaggacg gacaggatta
     1141 ttatttcctc gggcacgaac ggtttgagca gctcaaaaac gaaatggcct tcgccgaata
     1201 tgcccaggta cacgggaact ggtacgggac tcttaaaagt gaactggctg aaaaaacgcg
     1261 acccggggtg ctctgtatcc tggatatcga tgtccaggga gcagcccgcc tgagagcgct
     1321 ctatcccgac gccctcaaga tctttgtccg cccacccgac gttgccacac ttgaaaaacg
     1381 attaatgggc aggagtacgg aatcgccgga ggtgcgggct cttcgtgtgt ccaatgctgt
     1441 cagagaactt gaagatgagc atttttttga ttattctgta gtcaacacag attttgaaca
     1501 ggcatatgcc caagtgcagg agatcattct gcagcgcaaa ggagctttgg ataatggcca
     1561 ttccgtttga gaaattgctg aaccgcaatg aaaaccgcta tgagatgtgt gtcgctgcca
     1621 tgaagatttc gcagcatatt gctacaacca catcctccga agagatcgga aagatgcgtg
     1681 acaaacttgc agttttggcc atttcaaaac ttctggacga gaagatcccg atcaagactg
     1741 gagttgatgc tgcatccagg aaggtccggg acgattactg atgtaatcat tctcccatga
     1801 ccattcctat gatttgtgga ccaacagccg cgggaaagac agccgcggct tgtgctgttt
     1861 ttggagggaa ggatgttctc gcgatctctc tggattcgat gcaggtctac acggggatgg
     1921 acatcgggac agccaagccc gatccggagg aacgttcccg actggaacat gtgatgatcg
     1981 actgtgccga tccccgccag gtttggagcg tcgggaccca tctcaaacag acggaagcct
     2041 ggataacccg ggcgagggag tccggtcggc aggtcgttct ggttggagga accggtctct
     2101 atgtcaaggg tctgatcaat ggcctgtttg acggtcctgc ccgggatccg gcactgcggg
     2161 ccgaactcga ggaacgggaa aaaacccgac cgggcgtgct ctaccgggaa ctcctgggcc
     2221 gtgatcccga aacagccaaa cggctctcac aggcagatat ccgaagaatc atccgggcct
     2281 tggaggttct gaccctgact gggacgggga tcactcgtct gcaacgggaa cagactgtcc
     2341 ggaatccgct caagtttctc ctggccggat tgatcgtgga cagggatgaa ctggcccgac
     2401 gaatcgaaga gcgtgtcgac cggatgtatg gagccggtct tgtggatgag gtgcgggacc
     2461 tgctggactc cgggtgtcac gaagcattgc catccatgca gggcgtcggg tacagggaga
     2521 ccatcgcctt tctccgggga cggatttcac tggatgaggc ccgggcacag tgtatcgtca
     2581 atacccgatc gcttgctcaa aaacagatca tgctctttcg ccgcattccg gagctggttt
     2641 ggttccatgc ccgggatcat gagcggctgg cggcctatct tgcgggagga tccggatgaa
     2701 aggcggaaaa ctcttcggga tcatcgctct cggatacgaa cagcggcggc tctggccgct
     2761 tgatttggcc aaccgtccct ttgcctgcag accatttttc ggaggaaaaa cccttcttga
     2821 agcttcattc gaacggatgc gggtcctgat accggatgac cggatctggg tgattgctcc
     2881 gcagtcgctc ctccctctgg tcgcctcact cctgccagga ctggattcca gacggttgta
     2941 tgccggttcc ggcaccaatc cgggagatct gtacgccctg accgaaacca tctccctgat
     3001 cgatccggat gggcaggtcg tcttctctcc ggccgggatc attgccgggg atgaccaggc
     3061 attttccgat ctcgtccatg atgcggccag acttgcccat gcaacgggct gcatcgtcac
     3121 tggaaccgtc ccggtcagtg gaatcagggc cggctatcca tgcattcttc ccggggatgc
     3181 ctgtatccgg tccggacggg tcttcggtcg tgatatccgc aggtattttc cctcggttcc
     3241 gtccgccgat gcagaagcca tctcggcgcg tggcgctgtc tgggcaaccg gaatccatgt
     3301 ctggaataca gccacctttc tggtcgatgt tctcgggttt ctcgccggac agcaggatat
     3361 ggacgagtcc gacgatcttt cttcacggtt tttttcccaa tccaaaaact ggttgatggt
     3421 ggagtccgca ttggtttggg atccgatgcg ttccttcgag gatttggcgc gctatatccg
     3481 gggggacggt tcgggaaatt ccagaagtgg ccattgcatt gccggtcaga cgaggaattc
     3541 catcattctg tcggacaatc ccggtggctt ggtcgtcgtg cacggattgc atgatgtcct
     3601 cgtggtccag accggtgaga ccattctctg ttgttcacgg ggagatgaag agggagtcaa
     3661 ccgcctgttg tccagtcttg ccggaataca gcaggaagcc ggatcatgac agacagcaaa
     3721 aaaaataccc gcacacacgg gtttatgcgt actatttatt gactacgcgc actgttcctg
     3781 attccggggc agtgattcca gcataaggag cgtgcttcca tgccgtccgg aaaaaaacgc
     3841 aagcgcatga agatcgcgac acacaagcgc aagaagaagc gccgtgccaa tcggcacaag
     3901 aacaagaagc ggtaatctgt cgcatcagat gcatgaatga cgggagacgg tgttgctggt
     3961 ctcccgtttt tttctttttg gccgaaccag gtctgccgat actggtacgg gaatgcccta
     4021 gaactggagg atggacgatg ttcctcgcaa gcagaatgct cggttttgcc gcattgtgtc
     4081 tgtgcactgc gctcccgctt gcgggggtct ccctctggca ggatggtcat ctcttttcat
     4141 cccggggcaa ggttgttccg ggtgatattc tcaaaatccg tttttcgtac aggaaccttg
     4201 tccggtatcg aaccgagatg aaagcaggcg atacccaaaa gctcaatctt ggaaagccgg
     4261 atctgaaaac cttttccttt ttgccctcga tcgacaacaa cacgacctat aaccggaaca
     4321 acaatcttga gtacacaagc gagcgtgagt tcgcaacgac aattgccgtc atggtccaga
     4381 ccatcgcttc gaacgggatc atcagtttcc ggggaaatca cagtctccag ttgaacgggc
     4441 agaacgagga agtcgtcatc caagggcagg tccgttcgca ggatgtagcg gacggcaact
     4501 tcgtcgcgtc gacggatatc gcaaacctca attttgcctg gatcggaccg tccgtcatcc
     4561 ggcagaacgt acttcgaacc ggagatctga ctgtctcgac caacaccaac cagcgggccg
     4621 gccaggatgc ccctgaattg accaacgaag tcaagcgacg tttgctgatg cagtatctga
     4681 accggattca tgacattctg ttcaggccgt aggagattcc gatggcttcc atcctgatag
     4741 ttgatgattc cgcgtttgtg acaacccaac tcacccagat tcttgtcaag ggtggacaca
     4801 ctgtgtctgc tgcccttccg gtggagacga agcgatcgcc ttcctcgagg aaaacgcagg
     4861 ttccatcgat cttgtgacgc tggacataac gatgccggga acaagcggta tcgataccct
     4921 gcgccatatc cggatgcatt ggcccgggat actctgtgtg gtggtctcgg gtatcggaaa
     4981 aagggagaca gtgctcgaga cacgggaact gggcgcagcc ggctacattg tcaagccgct
     5041 cagtcgggaa cgggtcctgt caaaaattgg cgagattctc tccgagacca gggttgaatc
     5101 ctgatattcg cttgtgatag actggccaca gggtttcgat ccgtatgatc ctggtggagg
     5161 gtattcccga tgcgttctct gtttttggcg tttcttcttt ccggtgtttt tgtcgcgtgt
     5221 tcatcccaaa aaacacatga ttccctcgat gcggccattg cggcagccgg ttccaactct
     5281 cgggatatcc tcatcattgt ctcaaaatcc gatgagtgcc atgcctgcag tctcctcgaa
     5341 aaaaacatct tgaccaatcc ccagtggaaa cccgagtcgc tttacgaggt ggtcagaatc
     5401 gatttgaccg aaaaactcaa aaaagaacat cccgagttat ggaagagtgg tgataccatc
     5461 cagacgcggt atggcgcgcg ctggtttccg gccatcattc tcacggattc caggggcatg
     5521 ccctaccata tcggcgggta cgatgaatca agtcctgaag agtttttcaa attgctggaa
     5581 gggcggacaa aagaaaggcg gcatgaactg gccgcgattc ttgcaaaagc gactaatggc
     5641 atgaaggcca gggcggcgct tcacaagctc aaggagtggg gcgtggatac ggcataccac
     5701 aaactcaaag cccttgccat gggtggctca agggatcccg ggtttgccat cgagttggtg
     5761 gagtattgga cagccctcgg agacctgaaa aagcgggaag aatggcttcg cgtgctggaa
     5821 acgggatggc cacaagcctg gcgagatacg ctcgtccgat tggacgtcga gcagatcgag
     5881 aaaaacgagt tcgagaagat ggagtggaaa gccgcacgtg ccaagctcga gcctttgatg
     5941 gccaaaaagc cgcttggtat cgccgcatcc agattgtatg ccgctctggg cgaagtggcc
     6001 taccagttgc gcgagagagg accatgtcta ggctattttt cacaggcact tgaacatgca
     6061 ccggagggaa agctgaagac catgctcact aaccggttgg cgtatctgaa aaaaaattcg
     6121 ttctgaaagt cgggtgtcca tgcagagtgt tcttgccttc gatattggtg gaaccttcat
     6181 caaggcggcc agggtttcct cgaccgggga gattctccag cgtgtgcgtg ttccggtgtc
     6241 gccatcccgg gaggagatgt gtgccatcct ccgtcgcgtc gcgcaggatc ttctcggtga
     6301 tcatgagccg gagacaacag ggatcggagt gggtgcgccg ggagcgatcg acaggtcagt
     6361 cccc
//
LOCUS       DAOSHE010000076         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10726_length_6364_cov_3.912030, whole genome shotgun sequence.
ACCESSION   DAOSHE010000076 DAOSHE010000000
VERSION     DAOSHE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_6364_cov_3.912030"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..187
                     /locus_tag="PLD82_03360"
     tRNA            116..187
                     /locus_tag="PLD82_03360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            195..268
                     /locus_tag="PLD82_03365"
     tRNA            195..268
                     /locus_tag="PLD82_03365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:229..231,aa:Arg,seq:tct)
     gene            354..425
                     /locus_tag="PLD82_03370"
     tRNA            354..425
                     /locus_tag="PLD82_03370"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:His,seq:gtg)
     gene            491..564
                     /locus_tag="PLD82_03375"
     tRNA            491..564
                     /locus_tag="PLD82_03375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Arg,seq:tcg)
     gene            591..663
                     /locus_tag="PLD82_03380"
     tRNA            591..663
                     /locus_tag="PLD82_03380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:624..626,aa:Lys,seq:ctt)
     gene            669..748
                     /locus_tag="PLD82_03385"
     tRNA            669..748
                     /locus_tag="PLD82_03385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:702..704,aa:Leu,seq:tag)
     gene            773..844
                     /locus_tag="PLD82_03390"
     tRNA            773..844
                     /locus_tag="PLD82_03390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:805..807,aa:Gly,seq:gcc)
     gene            1504..2520
                     /locus_tag="PLD82_03395"
     CDS             1504..2520
                     /locus_tag="PLD82_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64460.1"
                     /translation="MRIPVVATALLVSSFVFAQNSSYQMGEQITIPEGGSESREVNAL
                     VISESRIVIPNTWRFVRTKKAVERNDSYSQAEFCRFNDQVNNVFGFFEYTTFNIESNI
                     DAEALAKLVASTLTSEVTDKILHKTFIGGRDAYIVSGRQKDKNWDYMTALIPEGKSYN
                     EIRLLANEGYLKSNPAMAHGILGSYRRMPEGWSERIIPNGIKFKSTDARWYWQDDWVS
                     ANFQGYTITDSASHETPIDYIGIARTERTDIEFVQALISGFNEVVIVPEFEVKVAVGD
                     RQMAGSALVSKNNNGLISLYYFFKQNGTSHLVMICYKTTSDDTMPEGIQTEIRNVLSK
                     YFYW"
     gene            2588..3943
                     /locus_tag="PLD82_03400"
     CDS             2588..3943
                     /locus_tag="PLD82_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64461.1"
                     /translation="MKMTWRCKYVLPVILGCLVFLGSASSRHAYSDVQTRGETVRSAT
                     VFNKWISAQAIREDVSFGSLVDDVTPDSTEYFIKQVPPAMRTQLLFMFPRTMRFTDMT
                     SDEIGKVATHLKKMAGFLESNIPGFVDYLAANKFTTAEINQADVQDMENGRLSDVYQK
                     YRRFLESMMFHGDMKKYYSSLNGIANRFLEYCFYPKTHAHFRNILDKSENHAVVRFLY
                     EIIWYYLARSEWRSWHQNTLDNLKREYDQGKEIVYIAGGSDIYQLLKRGIYRIRNIDP
                     LYPTQTKYYSEGWDFLAVGKGAGSGIGDRIVFDQDEFSGRKLVMRRISYKRTGTLLEN
                     VEIAETNVSIPKSETVWSIEDGDGKRLGTYTLERRFCTQNDFVTGPGRALLISFNELY
                     FIVTKNDENWGIKPELFAENFKFHVKQVRAPLSREVIMNMRAIQGSKYPNRFGSSVIG
                     H"
     gene            4168..4317
                     /locus_tag="PLD82_03405"
     CDS             4168..4317
                     /locus_tag="PLD82_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64462.1"
                     /translation="MRKNETVRREQGEKRDYVLIMPPKGSFLPGLSIAGLRDGRNPLP
                     RVRFC"
     gene            4378..5769
                     /locus_tag="PLD82_03410"
     CDS             4378..5769
                     /locus_tag="PLD82_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG64463.1"
                     /translation="MNEWNLLTQRLRRILGENAVLVGQDAEPYAADRWARGGVPPLVV
                     LPATQDDVSRAVKNLYDAGLPFVARGRGTGMTGGALPMGRAAVISFERMNRIIEVRPD
                     AFEMTVEPGALLSEVQAAAKSVGLLYPPDPASADECSIGGNIAENAGGLSCVKYGVTG
                     EYVLSLDVVLPDGRIATFGSHARKDVAGLDLKRIVIGSEGMLALVTRIVLRLVPRPEA
                     DATVCCWFSGAEEAVSSLQAVMQGGVMPARAELIARECLRIMADDGVSVHPGAGALLL
                     LGLDGTLDGVEEERKMAGRILEKRVLAMDSASGGDASHLWKLRSSLSPALKKLAPMKI
                     NEDIAVPLDRQADCIRFTEELAARTGLLIAVFGHAGDGNLHVNFMITPEEREIAEGAV
                     GELFTEVVRLGGTITGEHGIGVAKAPWAGLRLDPVTLELCRSLKLVFDPKNLLNPGKA
                     LYGAVPGEITRLV"
     gene            complement(5813..>6364)
                     /locus_tag="PLD82_03415"
     CDS             complement(5813..>6364)
                     /locus_tag="PLD82_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64464.1"
                     /translation="LEEVSRLGIFAKTEAEMALAHIHTYDLRDGYSGLRYTQALMERY
                     PHNPEFRLQYAENLLATGQSGKAIETVTKGLELTTSGWYRPTHRFRFICLMGKIHHTA
                     VRYHETLDWLNKAIAMKDQANRGNFLAWAYVRRGDALVHLGNRTFGLRDYHTAIELDP
                     AGSAGEAAQQRIKTANSTRDTRY"
BASE COUNT         1415 a         1499 c         1888 g         1562 t
ORIGIN      
        1 atgcgtgctc ctttgaggga ctgtctgaaa atatactgtg gaaaacctgc tggagttttc
       61 cccgaattta ttgaccatct ccgaggccgg gttgtagctt tgtggcaatg cagtacgggg
      121 tgtagcgcag gggtagcgca tctgctttgg gagcagagag tcgctggttc aaatccagtc
      181 accccgaatc tctcgcacca gtagctcagc tggatagagc aacagccttc taagctgtgg
      241 gtcacaggtt cgagtcctgt ctggtgcgcg cggtggccca tggcagcgaa tttgttctct
      301 ccttgtcgct tgccggatcg tgttgcctgt tgtatcttta ttcaagtttc atggtgggtg
      361 tagttcagcg gtagagcacc agaatgtgga tctgggggtc gcgggttcaa accccgtcac
      421 ccacccaaaa atgtcccata aaagtcttgc cttttgcggg ccattgatta gctttacaac
      481 gcttgtatga gcgtccgtag ctcagctgga tagagcgtcg gacttcgaat ccgcaggtcg
      541 caggttcgag tcctgccggg cgcgtacact caagcgcact ttcgcaagac gggccgctag
      601 ctcagctggt agagcagcag actcttaatc tgcgggtcga aggttcgatc ccttcgcggc
      661 tcactcgggc gagtgtggtg gaatggtaga cacgcctgac ttaggatcag gtgcctcagg
      721 catgggggtt caagtccctc cactcgcatc tcgtgcccgg tgtacagtac ttgcgggaat
      781 agctcagtgg tagagcgcca ccttgccaag gtggatgtcg cgggttcaag tcccgtttcc
      841 cgctcacaaa gatcgcccgt cgtggcggtc tttttttgtc ccgggagatc gagcttctga
      901 ctggggcctg aaactgtttt gcagggcata tgtccggtat gttgccaatg agcttgattt
      961 gcagatgatt gcctctccag tatttgaaga tctcagtgtg gtggaaaagg accgttcatc
     1021 agggttgtac cgggggccaa atcggggtcg tggcacatag gaaggcaagt atggccgaaa
     1081 atccttcgga cgaaatcgga acagtttgtg ggcatcggcg agtttctcct gctatggcgt
     1141 ccggaatttt cgtcacatgc ccaggctggt tcggtgaggc tgtcctgtat cctgcttccc
     1201 atctgagggc gttcaagtca agcgatgtgt ttcggacagg atcaccatac ttgatggctt
     1261 gctcaggacg ggctgctcga cgggacatgc gctgccacga tactcgagag ttttggaaat
     1321 acacgatctg cgaatcattt agttgtcgga tttcatgggt atgtcgaaaa ataatggcaa
     1381 attctgttga catcatatcg gaagaggggt agaaagatct gtctggcacg tgaaattttg
     1441 tgatcctgct gatatattgg gaaacgtgcc tgatgtaaaa cgagcactgg agggttgtac
     1501 accatgagaa tccctgtcgt tgccaccgcg cttcttgtga gctctttcgt ttttgcccag
     1561 aatagcagtt accagatggg tgagcagatc acaatccccg agggcgggag cgagtcccga
     1621 gaagtaaacg ctctcgtcat cagcgaatcc cggattgtca tcccgaacac ttggagattt
     1681 gtccggacca aaaaggctgt cgaacggaat gattcctact cgcaagccga gttttgccgg
     1741 ttcaatgatc aggtcaataa tgtgtttggc tttttcgaat acacgacatt caatatagaa
     1801 tcgaacattg atgcggaagc tcttgccaaa ttggtcgcca gcacattgac atccgaagtc
     1861 acggacaaaa ttctgcacaa gactttcatc ggtgggcggg atgcgtacat tgtatcaggg
     1921 cgccaaaagg acaagaactg ggactacatg acggctttga ttccggaggg gaagtcgtac
     1981 aacgagatac gtctcttggc aaacgaggga tatctcaaga gcaatcctgc aatggctcac
     2041 ggaattctgg ggtcataccg gcgaatgccg gaaggttgga gtgagcggat catccccaac
     2101 gggatcaaat tcaaatcgac agacgcccgc tggtattggc aggacgattg ggtcagtgca
     2161 aacttccagg ggtatacgat taccgattcg gcatcgcacg aaaccccgat tgactacatc
     2221 ggaattgccc ggacggaacg gacggatatc gaattcgttc aggcactgat ctccggcttt
     2281 aatgaggttg tcatcgttcc cgagttcgag gtgaaggttg ccgtcggaga tcgacagatg
     2341 gcaggatctg ctctggtcag caagaacaat aacggcctga tttccctgta ttatttcttc
     2401 aaacagaacg ggacttcgca tcttgtcatg atctgctata aaaccacctc ggatgatacc
     2461 atgccggaag gcatccagac cgaaatccgg aacgttttga gcaagtattt ttattggtaa
     2521 aagcgagagg gttcggcgcc attgccggtc gccggaccct gtgtgtccag cttgacaggt
     2581 gatgataatg aaaatgacat ggcgttgcaa atatgttctt cccgttattc ttgggtgtct
     2641 ggtttttctc ggctcggctt cctcaaggca tgcctattct gatgttcaaa cccggggcga
     2701 aacggtcagg agtgcgactg ttttcaataa atggatatcc gcccaggcaa tccgggagga
     2761 tgtcagtttt ggctcgctgg tcgatgatgt gacgccggat tctacggagt acttcatcaa
     2821 acaggttcca cccgccatgc gaacacagct cttgttcatg tttccgcgga cgatgcgttt
     2881 tacggacatg acgagtgacg aaatcggaaa agttgcgacg cacctcaaga aaatggcggg
     2941 attcctcgaa agcaatattc ctggttttgt tgattatctg gcagcaaata aatttacaac
     3001 tgccgaaatc aatcaggcgg atgtccagga tatggaaaat ggacgtctca gtgatgtcta
     3061 ccaaaagtac cggcgtttcc ttgaatcgat gatgtttcac ggcgacatga agaagtatta
     3121 ttcatccttg aatggcatcg ccaaccggtt ccttgaatac tgtttttacc cgaagaccca
     3181 tgcccatttt cggaacattc ttgacaagtc tgaaaaccat gcggttgtgc ggtttttgta
     3241 cgagatcatc tggtattatc tggcacggag cgaatggcgc tcctggcacc agaatacgct
     3301 ggacaatctc aaaagagagt atgatcaggg gaaggaaatt gtctacattg ccggcgggag
     3361 cgatatctat cagctcctca agcgtggaat atacaggata cgcaatatcg acccgctcta
     3421 tccgacgcag acgaaatatt actccgaagg atgggatttt ctggccgtcg gaaagggggc
     3481 cggaagcggg attggcgaca ggatcgtttt tgatcaggac gagttttccg gacgcaagct
     3541 tgtcatgcgc aggatctcgt acaaacgcac tggaactttg ctcgagaatg tggaaattgc
     3601 tgaaaccaat gttagtatcc caaagagcga aacagtatgg agtatcgagg atggagacgg
     3661 gaagaggctc gggacctata cgttggaacg gcgcttttgt acgcaaaatg attttgtgac
     3721 tggtcccggg agagcgcttt tgatttcttt caacgagtta tacttcatag tgacgaaaaa
     3781 cgacgagaac tggggcatca agcccgagtt gtttgcggag aatttcaaat ttcatgtcaa
     3841 acaggtccga gccccgctct cacgggaagt gatcatgaat atgcgagcca tccagggatc
     3901 caaatacccc aaccgttttg gatcgtctgt catcgggcat tgatgattca gtcttggttt
     3961 cccctgctac gtggtttcca cacagcagtc gggagtccgg gcggttttct ttgccctttg
     4021 ggcatggttt ctgccgacca ggatttctgg aaagctcggg acagcacaat cacaaacgga
     4081 atcctgaagc cggtccggcg aagaatggct tcagtcagaa ttgttttcct ccgattgttg
     4141 tagaggagtc tgccgaggag ggatgcgatg agaaagaatg aaacagtgcg tcgtgagcag
     4201 ggcgaaaaga gggattatgt cctgatcatg ccaccaaagg gaagttttct tcctgggttg
     4261 tcgattgccg gattacggga tggcaggaat cctctgcccc gggttcgttt ctgctgattc
     4321 tgcgggtatt tcgggcagat ttggtctctt cctgaatgcg ggggatgaac gggaaacatg
     4381 aatgaatgga atctgctgac gcagaggttg cggaggattc tgggagagaa tgccgttctg
     4441 gttgggcagg atgccgagcc gtatgccgcg gatcgttggg ccaggggagg ggttccacct
     4501 cttgtagtcc tgccggccac gcaagatgat gtttctagag cagtgaaaaa cctgtatgat
     4561 gccggtttgc cctttgttgc gcgtgggcgg ggaactggta tgaccggagg ggcgctcccc
     4621 atgggacggg cagcggtcat ttcctttgag cggatgaacc ggatcatcga ggtgcgtccc
     4681 gacgcgttcg aaatgacggt tgagccgggt gcgctcctct cagaagttca ggcagccgcc
     4741 aaatcggtcg ggttgctgta tccgccagat ccggccagcg ccgatgagtg ctcgattggc
     4801 gggaatatcg ccgaaaatgc cggcggactc tcttgtgtca agtacggtgt gacaggcgaa
     4861 tacgttcttt cactggatgt ggttttgccg gatgggcgga ttgccacatt cgggagtcat
     4921 gcccgcaagg atgtcgccgg attggacctc aagcggattg tgatcgggag cgagggaatg
     4981 ttggctctgg tgacgcggat cgtgctgcgt cttgttccac ggcccgaggc ggatgcaacc
     5041 gtctgttgct ggttttccgg ggcggaggaa gcggtttcct cattgcaggc ggtgatgcaa
     5101 ggcggggtga tgccggcccg tgcagaattg attgcacggg agtgtctgcg gatcatggcg
     5161 gatgacggag tatctgtcca tcccggggcc ggcgcgctgt tgctcttggg tctggatggg
     5221 accttggacg gggtggaaga ggagcggaaa atggcaggcc gcatccttga aaagcgggtc
     5281 cttgccatgg actctgcatc aggtggcgat gcatcccatc tctggaagtt gcggagttcc
     5341 ctctctccgg ccctcaaaaa actcgcacca atgaagatca atgaggatat cgccgttccg
     5401 ctggacaggc aggccgattg tatccgtttt accgaggaac ttgcggcacg aaccggcttg
     5461 ttgattgcag ttttcggtca cgcgggagac gggaatctgc atgtcaattt catgattact
     5521 cctgaggagc gggaaatcgc ggaaggcgct gtcggagaac tctttacaga ggttgtccgt
     5581 ctcggtggaa cgatcaccgg ggaacacggc atcggtgtgg ccaaggcacc atgggcaggc
     5641 ttgcgtcttg acccggtgac gctggaactt tgccgttccc tcaagctggt gtttgatccg
     5701 aaaaacctgt tgaatcccgg gaaggctctg tatggagccg ttcccggaga gatcacccgc
     5761 ttggtgtaag acaggctacg ggcagtgcct gaaaacggga gaatcgctca agtcagtacc
     5821 gcgtgtcacg ggtggaattc gctgttttga tccgttgctg tgcggcttct ccggccgaac
     5881 cggcaggatc aagctcgatg gcagtgtggt agtcccgcaa accaaaggtc cggtttccca
     5941 gatgcaccag tgcatcaccg cgccggacgt aggcccaagc cagaaaattt ccacggtttg
     6001 cctgatcctt catggcgatg gccttgttga gccagtccag tgtctcatgg tagcggactg
     6061 cagtgtgatg gatttttccc atgaggcaga tgaaacggaa acggtgtgtc ggtcgatacc
     6121 agccactggt ggtcagttcg agccctttgg tgacagtctc gatggctttg ccggattgac
     6181 ctgttgcgag aaggttttca gcgtattgca ggcggaactc gggattatgc gggtagcgtt
     6241 ccatgagtgc ttgtgtatag cgcaaaccac tgtatccgtc ccggagatcg taggtatgaa
     6301 tgtgggccaa cgccatttcg gcctcggttt ttgcgaagat accgagacgg ctgacttctt
     6361 cgag
//
LOCUS       DAOSHE010000077         6343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10777_length_6343_cov_4.222169, whole genome shotgun sequence.
ACCESSION   DAOSHE010000077 DAOSHE010000000
VERSION     DAOSHE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10777_length_6343_cov_4.222169"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLD82_03420"
     CDS             <1..344
                     /locus_tag="PLD82_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64465.1"
                     /translation="ELKKAVENLDKLVAMYDRLSRAEALELAAADDTINAQETVQEAT
                     RKELEGVWNTVRAHESAAQLARDELANAYDTIRAHEEVELFASQEVRLLREQLEDEQS
                     ADRKRTDHGTE"
     gene            331..2382
                     /locus_tag="PLD82_03425"
     CDS             331..2382
                     /locus_tag="PLD82_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64466.1"
                     /translation="MEPSEIARREDLSRIIEMYEHVFDLSRDELKNAYNTISAHEAVE
                     GLSRDELLQVKEEIRQIRYRDEVRSAFFSNVSHEIRTPLTLLLAPLESMLQGELGQFS
                     DEQRTYLKLMYENSRRLLRMVNDLLDFSKIESGKAKVWWREDDLVDLVQELLETYLPI
                     IERYELKMTVQMPSHPVMVFMDSEKIEKTINNLVFNAFKYVKHGGHIRIRIWDTTEHV
                     VFSVFNSGEPLPDEVLASVFDRYAQFEQYSDKRNIQGSGLGLALAREFVSMHQGRLRM
                     QNLRGHGIVCSFSLKKGSDHICREELEMGWGEFERRRQRTDRPVPQQGVSSLFSEIDD
                     FYSLDRSDLAEFEERIKDAAPSGLYSGESKRTVLVVEDNAEMRGFLKYILSGYFDVID
                     AVDGEDGVHKAKAFSPNLIISDVMMPRMNGHQMTRALKADEDTCRIPVILLTARANSL
                     NAMVEGLNIGADDYISKPFNIKELIARANALLRMGSLNSQLENTESVIFSLANAVEAK
                     DAYTEGHCFRLAELSAEIARRAGLPETDIRRIKNGAILHDVGKIGIPEAVLTKPGKLT
                     DEERTVIRQHPVIGERICRPLRSTSSIISIVRSHHERWDGHGYPDGLSGETIPVEARI
                     VSIADSFDAMVFDRVYRPALGLDRARSIFREERDRGQWDPHLVDIFLSIHESWLKGNM
                     L"
     gene            2379..3359
                     /locus_tag="PLD82_03430"
     CDS             2379..3359
                     /locus_tag="PLD82_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOG64467.1"
                     /translation="MTPEPMQDQRVFDLLSGVSRTFALSIVVLRGTLASDVATAYLLC
                     RLLDTVEDDANLPATERRHVLSGLADGIGNPAQEAEALELLAGLEGRITATPVELELL
                     AEAPALFARIKRMDAQRGQVLRRWAEEMARGMAEFVSDTPGASIHTLQDLARYCHYVA
                     GTVGGMLTGLFALTGPGVTAQRQRRLETHMEAFGRGLQMVNIIKDCGKDWSMGRCFLP
                     GELAGNEAGFRGAHPHLLGELVKQAEVDLDDAVRYIRNLPRRMWRARLFCVYPLVFAR
                     KTLRILAGNQARTGEQGPKISRKDVKRAMLFSAPAAFSNLWLRLVCRRQT"
     gene            complement(3350..3967)
                     /gene="thrH"
                     /locus_tag="PLD82_03435"
     CDS             complement(3350..3967)
                     /gene="thrH"
                     /locus_tag="PLD82_03435"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOG64468.1"
                     /translation="MHTVCLDMEGVLFPEIWINVAKKTGISELERTTRDEPDYDRLMQ
                     YRMDILRREGVTLGDIQAVIATLTPLEGVPEFMEWLRMHFQVLILSDTFYQFAMPLVS
                     QIGSPTIFCHNLMTDDAGMISGYRLRQPDAKRAVVDALRHDLKMRVIAMGDSYNDTSM
                     LMAADQGVLFCPPQNVIDEFPQFPVAWMYEDARRFFSEANDRFQV"
     gene            4121..>6343
                     /gene="secA"
                     /locus_tag="PLD82_03440"
     CDS             4121..>6343
                     /gene="secA"
                     /locus_tag="PLD82_03440"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG64469.1"
                     /translation="MVQMVIRALFGTKGERDIKRIRPIIERINALEPEMQALDDAALK
                     GKTAEFKGRIREMLGCDPKELVLHGDYALRDRRRLLDALDMILPEAFAVVRETAWRTI
                     KQRPYDVQLVGGIVLHQGKIAEMKTGEGKTLTSTLPVYLNALTGLGVHVVTVNDYLAR
                     RDAEWMSPIFHFLGMSVGFILTNMDPRQRQHSYSCDITYGTNNEFGFDYLRDNMVLHP
                     DLRVQRGFYYCIVDEVDSILIDEARTPLIISGAAEESTKKYYEVQRIMPRLTRGFTHG
                     QLIDRKLDALTDEKTELHTQLDAGEAVGREDQINARLEEIKNEIRELNREKQMAAMNK
                     KLNVPSDEGDYIVEESDRAAWLTPNGVKKVESLLGIDNLYGNTHMDLVHHIEQALKAN
                     TIFHVDVDYVVKDGEVLIVDEFTGRLMPGRRYSDGLHQALEAKEGLTVARENQTLATI
                     TFQNYFRMYVKLSGMTGTAETEAEEFGKIYSLDVVAIPTNRKIQRIDNPDRIYRTENE
                     KLNAIAEEIKRLHEQGQPVLVGTISVEKSERLSRLLKGRNVPHNVLNAKFHELEADIV
                     SQAGQYGRVTIATNMAGRGTDIILGGNPELQGRKLIENTLKNTGRKAETEEVNEFVRL
                     VLLGNAPALDAWCKEHTVFDEDLANKVRTLHRECRENQKSVLEAGGLFILGTERHEAR
                     RIDNQLRGRSGRQGDPGASRFYISMEDDLMRLFGGERLKNIMQRLGMREGEEIEHSLI
                     S"
BASE COUNT         1473 a         1828 c         1819 g         1223 t
ORIGIN      
        1 aagagttgaa aaaggccgtt gaaaatctgg acaaactggt cgccatgtat gaccgtctct
       61 cccgagctga agcgcttgaa cttgcagccg ccgatgatac gatcaacgca caggagaccg
      121 tgcaggaagc gacccgcaag gagctcgaag gcgtctggaa cacggtccgg gcccacgaat
      181 cggcggccca actcgccaga gacgaactgg ccaacgccta cgataccatc cgggcccacg
      241 aagaggtcga gctctttgcc tcgcaggaag tccggcttct ccgtgagcag cttgaggatg
      301 aacagagtgc cgacagaaaa cggacggatc atggaaccga gtgagatcgc ccgacgtgag
      361 gatctatccc ggatcatcga gatgtacgaa cacgtctttg atctctcgcg ggacgagctg
      421 aagaatgcct acaataccat cagcgcccat gaggcggtcg agggactttc ccgtgacgag
      481 ttgctccaag ttaaggaaga gatccgccag atccgctacc gggacgaggt ccgcagcgcg
      541 tttttttcca atgtctccca cgagatccgc accccgctga cactcctgct tgcacccctt
      601 gaatccatgc tccaaggcga gctcggccag ttctcggatg agcagcgcac ctatctcaaa
      661 ctgatgtacg aaaactcccg ccgtctcctg cggatggtca acgatctgct ggatttttca
      721 aaaatcgaat ccggcaaggc caaggtctgg tggcgcgagg acgatcttgt ggacctggtc
      781 caggagcttc tcgaaaccta tcttccgatt atagaacgct acgaattgaa aatgacggtc
      841 cagatgccgt cccatcccgt gatggtcttc atggactccg aaaagatcga gaagaccatc
      901 aacaatctcg tcttcaacgc cttcaagtac gtcaaacatg gcggccatat ccggatccgg
      961 atctgggaca cgaccgaaca tgtcgtcttt tcagtcttca acagcggcga gccgttgccg
     1021 gacgaagtcc tggcctcagt gtttgaccgc tacgcccagt ttgagcagta ctcggacaaa
     1081 cggaacattc aggggagtgg tcttggcctg gccctggcgc gcgaatttgt ttcaatgcat
     1141 cagggacgcc tgcggatgca gaatctgcgg gggcacggta ttgtctgcag cttttccctc
     1201 aaaaagggaa gtgaccacat ctgccgcgaa gagctggaga tgggctgggg cgaatttgag
     1261 cggcggcgtc agcggacgga ccggcctgtc ccccagcagg gtgtctccag cctgttttcg
     1321 gagattgatg acttttattc ccttgaccgg agtgacctgg ccgagtttga ggagcgcatc
     1381 aaggatgctg cgcccagcgg gttgtacagc ggagaatcaa aacgtacggt gcttgttgtt
     1441 gaggacaatg ccgagatgcg cgggtttctc aaatacatcc tgtcgggata ttttgatgtc
     1501 attgacgcag tggacggaga ggacggggtc cacaaggcca aggcgttttc cccgaatctc
     1561 atcatctccg atgtcatgat gccccgcatg aacgggcatc agatgacccg cgccctcaaa
     1621 gccgacgagg acacctgccg catccccgtg attctcctga cggctcgggc caacagcctg
     1681 aacgccatgg tcgaaggact caatattggg gccgatgact atatctccaa accgttcaac
     1741 atcaaagaat tgatcgccag ggccaatgcc ctcctgcgca tgggttcctt gaacagccag
     1801 ctggaaaaca cggagagtgt cattttttcc ctggccaatg ccgtcgaggc caaggacgcc
     1861 tataccgagg gacattgctt cagactggcc gaactctccg cagagatcgc ccgcagggcg
     1921 ggcctgcccg aaacggatat ccggcggatc aagaatggcg cgattcttca cgatgtcggg
     1981 aagatcggga ttccggaagc cgtcctgacc aaaccgggca aactgaccga cgaggaacgg
     2041 accgtcatcc ggcaacaccc cgtgatcggg gaacggatct gccggcccct gcgctcgaca
     2101 agcagcatca tcagcattgt ccgatcccat cacgaacgct gggacggaca cgggtatccc
     2161 gatggcttgt ccggcgagac gattccggtc gaagcacgga ttgtttccat agcggacagc
     2221 ttcgatgcga tggtctttga ccgggtctat cggccggccc tgggactgga tcgggcacgg
     2281 agcatttttc gcgaagaacg ggatcgtggg cagtgggacc cgcatctcgt ggacatcttt
     2341 ttgtccattc atgaaagctg gctcaagggg aacatgctgt gacacctgag ccgatgcagg
     2401 accagcgggt ttttgatctt ctctccgggg tctcccgaac ctttgctctc tctatcgttg
     2461 tcttgcgtgg gaccctggcg agtgacgttg ccacggccta tctcctctgc cgccttctgg
     2521 atacggtcga ggacgatgcg aacctgcccg cgacggagag acggcatgtc ctctccggac
     2581 tggctgacgg aatcgggaac cccgcacagg aagccgaagc gctggaactc ctggctgggc
     2641 tcgagggaag aatcacggcc accccggtcg aacttgaact tttggcagaa gcccccgcac
     2701 tctttgcgcg gatcaagcgg atggatgcac aacgcggaca ggtgctccgg cgctgggcgg
     2761 aggagatggc ccggggaatg gcggaatttg tcagcgatac acccggagct tccattcaca
     2821 ccctccagga tcttgcacga tattgccact atgttgccgg aaccgtgggc gggatgctga
     2881 cagggctgtt tgcgctgacc ggccccggag tgacggcaca acgtcagcgc cggctcgaaa
     2941 cgcacatgga agcgtttggc cgcggcttgc agatggtgaa catcatcaag gattgcggaa
     3001 aggactggtc gatgggacgg tgtttccttc ccggagagct ggccggaaac gaggccggat
     3061 tccggggcgc ccacccacac ctccttggcg aactggtcaa gcaggccgaa gtcgatctgg
     3121 atgacgctgt ccgctatatc cggaacctgc cccgcagaat gtggcgggca cgactctttt
     3181 gcgtctatcc tctcgtgttt gcccggaaaa ccctccggat cctggccgga aaccaggcca
     3241 gaaccgggga gcagggtccc aagatcagcc ggaaggatgt caaacgcgcc atgctcttct
     3301 ccgcccccgc tgccttcagc aatctctggt tgcgtcttgt ctgcaggcgt cagacttgaa
     3361 aacggtcatt cgcctcgctg aaaaaccgtc tggcatcctc atacatccac gcaaccggaa
     3421 attgcggaaa ctcgtcaatg acgttttgcg gcgggcaaaa cagcacaccc tgatcggcgg
     3481 ccatcaacat gctggtgtcg ttgtaggagt cacccatcgc gatcacccgc atcttcaggt
     3541 catgacgcag ggcatccaca acggcccgct ttgcatcggg ttgtctgaga cggtaccccg
     3601 agatcattcc cgcatcatcg gtcatcaggt tgtgacaaaa aatggtgggt gaaccgatct
     3661 gtgacaccag cggcatggca aactggtaaa acgtgtccga gagaatcagc acctgaaaat
     3721 gcatgcggag ccattccatg aattcgggaa caccctcaag cggagtgagc gttgcaatga
     3781 ccgcctgaat atcccccagg gtcacacctt cccggcggag gatatccatc cggtactgca
     3841 tcaatctgtc gtaatcgggt tcatcccggg tcgtccgttc cagctcgctg atcccggttt
     3901 tcttggcaac attgatccat atctcgggga aaaggacgcc ctccatatca agacagacgg
     3961 tatgcacagc tcatcctccg ggcagggatt gttttgctat aaatctatcc ggcaggtgct
     4021 ggccgggcaa gccgagtctg cccgggttga aagacgcctg cccgctgcgt accttattgt
     4081 atgaaatgca cgcatagcct gtgaaacgga gatacaatac atggtacaga tggtcattcg
     4141 cgcactcttt ggtacgaagg gtgagcggga tatcaagcgc atcaggccga ttattgagcg
     4201 catcaacgct cttgagcccg agatgcaagc gctggacgat gcagccctca aaggaaagac
     4261 cgcagaattc aagggacgga tccgcgagat gctgggctgt gatcccaagg aattggttct
     4321 ccacggagat tatgccctgc gcgacagacg gcggttgctg gacgcactgg acatgatcct
     4381 ccccgaggca ttcgcagttg tccgggagac agcctggcgc accatcaagc agcggcccta
     4441 cgatgtccag ctcgttggcg gtattgtcct gcatcagggc aagatcgccg agatgaagac
     4501 cggtgaaggc aagaccctca cctcgacact ccctgtctac ctgaatgcct tgacgggcct
     4561 gggcgtgcac gtcgtaacgg tcaacgacta cctggcccgg cgcgatgccg agtggatgag
     4621 tccgattttc catttcctcg ggatgagtgt cggattcatc ctgacgaata tggatcccag
     4681 gcagagacag cattcctact cctgcgacat cacctacgga acgaacaacg aattcgggtt
     4741 cgattatctg cgcgacaaca tggttctgca ccccgacctg agagtgcagc gcggtttcta
     4801 ttactgcatt gttgacgagg tggactcgat cctgatcgac gaagcacgga cccccttgat
     4861 catctcgggt gccgccgaag aatcgaccaa aaaatattac gaagtgcagc ggatcatgcc
     4921 gcgcctgacc cgtggcttca cgcatggtca gctcatagac cggaaactgg atgccctgac
     4981 ggacgaaaag accgagctgc acacccagct tgatgccgga gaagcggtgg gccgcgagga
     5041 ccagatcaac gccagactgg aagagatcaa aaacgagatc cgggaactca atcgggaaaa
     5101 acagatggcg gccatgaaca agaaattgaa cgtgccgtcg gacgaaggag actacattgt
     5161 cgaggagagt gaccgagccg cctggttgac gcccaacggt gtcaaaaagg tcgaatccct
     5221 cctcggaatc gacaatctct acggcaacac ccacatggat cttgtgcatc acatcgagca
     5281 ggccctcaag gccaacacga tcttccatgt ggacgtggat tacgtcgtca aggatggcga
     5341 agtcctgatc gttgacgagt tcaccggacg cctcatgcca ggccggcgct acagtgacgg
     5401 actgcatcag gccctcgaag ccaaggaagg cctgacggtc gcccgggaaa accagaccct
     5461 ggccacaatc acgttccaga actatttccg gatgtatgtc aaactctccg gtatgaccgg
     5521 tacggccgaa accgaggccg aggagttcgg caagatctac agtctggatg tcgtcgcgat
     5581 tccgaccaac cgcaagatcc agcgtatcga caaccccgac aggatctacc ggaccgaaaa
     5641 cgagaaactg aacgccattg ccgaggagat caaacggctc cacgagcagg gacagcctgt
     5701 tttggttgga acaatatctg tcgagaagtc cgagcgtctc tcccggttgc tcaaggggag
     5761 aaatgttccc cacaatgtcc tgaacgccaa gttccacgaa ctcgaggcgg atatcgtctc
     5821 gcaggccgga caatatggcc gtgtcaccat cgccaccaat atggccggcc gcggaacgga
     5881 catcatcctc ggtggaaacc ccgaactcca gggtcgaaaa ctgatcgaga ataccctcaa
     5941 gaacaccggg agaaaagcag aaaccgaaga ggtgaacgaa ttcgtccggc tcgttcttct
     6001 cgggaatgca ccagcccttg acgcctggtg caaggaacac acggtgtttg acgaggatct
     6061 cgccaacaag gtccggaccc tccatcggga gtgtcgcgaa aaccagaagt cggttcttga
     6121 agccggcggt ctcttcatcc tcggtacgga acggcacgag gcaagacgca tcgataacca
     6181 gttgcgcggc cgaagcggtc gccagggcga ccccggtgcc tccagattct atatctcgat
     6241 ggaagacgac ctgatgcgcc tgttcggagg cgaacgactc aaaaacatca tgcaacgcct
     6301 tggcatgcga gagggtgagg agatcgagca ctccctgatc agc
//
LOCUS       DAOSHE010000078         6297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10890_length_6297_cov_4.365107, whole genome shotgun sequence.
ACCESSION   DAOSHE010000078 DAOSHE010000000
VERSION     DAOSHE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_6297_cov_4.365107"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLD82_03445"
     CDS             <1..415
                     /locus_tag="PLD82_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG64470.1"
                     /translation="IAIHNFPEGLATFMAGLSEPGLGISIAVAIAIHNIPEGVAVSAP
                     VYYATKSRKKAFILSFLSGLAEPLGALIGYFFLSSFFGPVTMGLIFAGVAGVMVYISL
                     DELLPTAEEYGEHHVAIAGVVAGMLVMAVSLLLLR"
     gene            complement(423..1001)
                     /locus_tag="PLD82_03450"
     CDS             complement(423..1001)
                     /locus_tag="PLD82_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG64471.1"
                     /translation="MDTVIRSRLVTENILILPESLYTRPSGDFVPLVKLYPGEDFDGQ
                     HEDRDVPRHKAEIIEWCLSHAAAFSETSEDIRRRIAVSLVAREATLSCGIGNGLALPH
                     CKTALIENPLELVLAVSPGDVEWYSADGTKTRIILIALFKANMPEAIPHRMQVSVVLA
                     RMLRETGLVARIQNMRDPAAIAAACSEALDQL"
     gene            1145..2365
                     /locus_tag="PLD82_03455"
     CDS             1145..2365
                     /locus_tag="PLD82_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG64472.1"
                     /translation="MIILHCADLHLGRRPQGGRGTWSANRYDDYFKAFSGIASFAVQR
                     GVDCLTIAGDIFDSNSMLPATLARTESILEQLKENGIPVVVTWGNHDRPGYGGDSEFW
                     LAYLVEKGLVLMPRAERSTTTSGPVWNFSACRLGEVSFWTPGWWMSQNEAALAALAGY
                     LATVPGPHVVLAHIAISGRDLIDQGAVAAEHILPFKELVVFMGGGHFHSFSHYPREAP
                     FFFVPGSPEYFDIAERASEKAVVLFNTENGEWERVPVKPRPKHELSLDTDTADFETFL
                     HTRLQPLLAEIRAEEGDMIRLEVRCAAGIWLDAGRCESWLEETGCFGKASVILRHSGV
                     DGSGEDSAAGQERPVEEIIREFVMTREIFSDCPDETMRLLNGLATGLRETTRSGDAIM
                     ERIEEFCTATKGKT"
     gene            2365..5205
                     /locus_tag="PLD82_03460"
     CDS             2365..5205
                     /locus_tag="PLD82_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOG64473.1"
                     /translation="MRISRIKLVNFRCHSVFEKELPAGINLLIGKNGSGKSSVLEAIG
                     ILFCDDSGRGGKLEDTVGPKKKSADIEVEFLAKDGSRWLVKRRIGQGSHSNRLFRWQG
                     EKWQPDETSAISSLVGLETSGSKEVFRSMIIAEQNRFVQPFLGTSTERTTVFDRVFGI
                     SQWRELGARADLEEPFKKAVAGLEAKKAMLEEESGLVEERGLDGREDHLKTELSRLHT
                     RMGLLSTANQAVMTCTEALRARDAALEETARLGERRDEIQNELTALMADREWLAGHEA
                     ALAGLAGLERALNGVRACREAALQVEAAGTNIRGTESRLADARLELERVVGEVSRMGA
                     ADKDYEAADRISAPLVVALESAWDELAPLQERSAAARAALEATTGRGEDAAELASRRE
                     RLLAEHSRYQELQALRESLLREKEDVQARHKALQEQRQILGTGRCPLLGETCRNLAGR
                     GSDAEGEIGRLLEELSLGTRQAAERLNEVTAEMARFEKAGGQLGAVTQAIAEAEKRET
                     ERREREVRLREIETVCRQAEERFDAVASSLANIPGFHPLKQAIPERIWSSRGQDRQVF
                     LEHLRKAQTAWQAGWRLEREQLTALVGERASRQKRCEDGESAVRADRADLERARDAYR
                     SVWRRIRDEEGVGQFVQTLPPEPDLAALTVLEDELDARRTPLASLREEAGARRGRLAG
                     EEGLHDRLGQLEARRKEEDGILRERETGFDAAASVLALSCRAIGEPGPAAPAVENLES
                     LSALTREIGLLREDVSNAIGQTSQALEGLRQEQENLRRLRESLASTLEHLGEARRRLD
                     LVQRIRGLLKDLGPWISKYALPLIARDATRHFRRITGRSERILWTHTAKDEYLVSLAE
                     SEEDSGVRSFVQLSGGEQVAVALAVRAALVESLSSSGFVIFDEPTINLDIESRRNLAG
                     AFKAVFGKIEQAFVITHDDSFEAMADNEIRFD"
     gene            5377..>6297
                     /locus_tag="PLD82_03465"
     CDS             5377..>6297
                     /locus_tag="PLD82_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64474.1"
                     /translation="MPGAHAIIHGEGRIPESEYRFFFVTNVAFLFGFVSHAIFLTVFS
                     FLGVPEMVLHNIASCFVFFIAILANQQRRYRLAMSLAYFEVMIHAAMAVVFIGQDSGF
                     QYYVFVVVPLIFYISVVPAWVRFMMAVVAFLVYAFISYFLRGEPVYSVDPWVLRFIRG
                     GNIAGVVAGFSFIGYWYGRINMIAEAALFAERNKLREKNERMQSELEMARRIQLQLVP
                     AVGPVKGLAFEYRPMTIIGGDFYDFVTFVGRPLEIGIFISDVSGHGVPAALIASMLKS
                     TLLQSVDILDDPPALMTRLNDTLVSKTNDNF"
BASE COUNT         1211 a         1767 c         2035 g         1284 t
ORIGIN      
        1 tatcgccatc cacaactttc ccgagggctt ggccaccttc atggccggtc tctctgaacc
       61 aggcttgggg atcagtattg ctgtcgccat tgccatccac aacatccccg agggtgtagc
      121 cgtttcggcc cccgtctatt acgcgacaaa gagccggaaa aaagccttca tcctgtcgtt
      181 tctctccggg ttggccgagc cgctcggcgc cctgatcggg tacttcttcc tgtcgagttt
      241 cttcgggccg gtcaccatgg gtctgatctt tgccggagtc gccggcgtga tggtctatat
      301 ctccctcgat gaactcctgc cgacggcgga ggaatacggg gagcaccatg tggccatcgc
      361 cggagtcgtg gcgggcatgc tcgtgatggc cgtcagcctg ctcctgctcc ggtaggccgg
      421 gttcagagct gatcgagtgc ttcggaacag gcggcggcga tggcagccgg gtcccgcatg
      481 ttctggatcc tggccacgag tccggtctcg cggagcatgc gggccaggac gacggagacc
      541 tgcatgcggt gggggatggc ctcgggcatg tttgccttga agagggcgat caggatgatc
      601 ctggtttttg tcccgtcagc ggagtaccac tcgacatccc cgggcgaaac ggcgaggacc
      661 agttccagcg ggttttcgat gagggcggtc ttgcagtggg gcagggccag cccgtttccg
      721 attccgcagg agagggtcgc ttcgcgggcc acaagcgaga cggcaatccg cctgcggatg
      781 tcttcgcttg tttcggaaaa tgcggccgcg tgggagaggc accattcgat aatctccgct
      841 ttgtggcggg gcacatcccg gtcttcatgc tgtccgtcga aatcctctcc gggatagagc
      901 ttgaccaagg gcacgaaatc tccggagggc cgggtgtaca agctctcggg aagaatgagg
      961 atgttctccg tgacaagacg gctgcggatg acggtgtcca agtgttccat ccggccagta
     1021 ttgaccggag agtacaagat tgcaagaaaa atggaaattt cctgacggtc tgtcccggga
     1081 aaacagtcca ggactgcatc aagccggccg gatcctgcgt atactcaatg cgacggagga
     1141 actgatgatc attctgcact gtgcggatct ccatctgggg cggcgtcccc agggaggacg
     1201 tggaacctgg tctgcaaacc gctatgacga ttacttcaag gcgttttcag ggattgcatc
     1261 cttcgcggtc caacgcggcg tggattgtct gactattgcc ggcgacatat ttgacagcaa
     1321 ttccatgctt ccggccactc tcgcccggac cgagtccatt cttgaacaat tgaaggaaaa
     1381 cgggataccg gtcgtcgtga cctggggaaa tcatgaccgg ccgggctacg gcggggacag
     1441 tgaattctgg ctggcctatc tggtcgagaa aggtctcgtg ctgatgcccc gtgccgagcg
     1501 gagcaccacc acatccgggc cggtctggaa tttttcggcg tgtcgtctgg gagaggtcag
     1561 cttctggact ccgggctggt ggatgtcgca aaacgaggcg gcgctggccg ctctggccgg
     1621 atacctcgcg accgtccccg gcccccatgt cgtgctggcc cacatcgcca tcagcggcag
     1681 ggatctgatc gaccaagggg cggttgctgc cgaacacatc ctccccttca aggaactggt
     1741 agtattcatg gggggtgggc attttcactc cttctcgcat tatccgcgcg aggctccgtt
     1801 cttttttgtt cccggttcgc ctgagtattt tgacatcgcg gagcgggcat ccgagaaagc
     1861 agtcgttctt ttcaatacgg aaaacgggga atgggagcgt gtcccggtca aaccgcgtcc
     1921 caagcacgaa ctctccctcg acacggatac ggcggatttc gagaccttcc tgcacacccg
     1981 actccaacca ctgcttgcgg aaatccgcgc ggaggagggg gacatgatcc gcctcgaagt
     2041 gcgctgtgct gcggggatct ggctggatgc cgggcgctgt gaatcctggc ttgaagaaac
     2101 agggtgtttc gggaaagcct ccgttattct ccggcattcc ggtgtggatg gttccggtga
     2161 ggattccgct gcgggccagg aaaggcccgt ggaagagatc atccgtgaat tcgtcatgac
     2221 ccgcgagatc ttcagcgact gtccggatga gacgatgcga ctcctgaatg gcctggccac
     2281 gggtctccgg gagacaaccc ggtcgggcga tgccatcatg gaacggatcg aggaattctg
     2341 cactgcgaca aaggggaaga cctgatgaga atctcccgga tcaaactcgt caatttccgc
     2401 tgtcattcag tttttgaaaa agaactaccg gccggaatca acctcctgat cgggaaaaac
     2461 ggttccggga aatcctcagt ccttgaggcg atcggcatcc tgttctgtga cgacagcggg
     2521 cggggtggaa agcttgaaga taccgttggt cccaaaaaaa agtcggcgga tatcgaggtg
     2581 gaattcctgg ccaaggatgg aagccgctgg ttggtcaagc gaaggatcgg gcagggctct
     2641 cattccaacc ggctctttcg ctggcagggc gaaaaatggc aacctgacga aaccagcgcc
     2701 atctcctccc tggtcggact cgagacaagc ggatccaaag aagtcttccg gtcgatgatc
     2761 atcgccgagc agaacaggtt tgtgcagccc tttctcggaa cctcgaccga acgaacgact
     2821 gtctttgacc gtgtgttcgg catcagccag tggcgtgaac tcggagcacg tgccgatctg
     2881 gaagaaccct tcaaaaaagc ggtggctggc cttgaagcga aaaaggcaat gcttgaggag
     2941 gagtccggtc ttgtcgagga acggggcctg gacggacggg aggatcatct caagacggag
     3001 ctttcccgtt tgcacacacg gatgggcctc ctctccaccg cgaatcaggc ggtcatgacc
     3061 tgcacggagg ccctccgggc ccgggatgcc gcccttgagg agacggcccg actcggggag
     3121 cggcgggacg agatacagaa tgaactgacc gcgctcatgg ctgacaggga atggctggcc
     3181 ggtcatgaag cagcgcttgc cgggcttgcg gggcttgagc gggccctgaa tggggtccgg
     3241 gcctgtcgcg aggcggcgtt gcaggtcgaa gcggcgggaa caaatatacg gggaacagag
     3301 tccaggcttg cggatgcccg cctcgaattg gaacgggttg ttggcgaggt cagccggatg
     3361 ggagcggcag acaaggacta cgaggcagct gaccggatct cggcccccct cgtggtcgcg
     3421 ctggaatcag cctgggatga attggccccc ctgcaggaaa gaagcgcggc cgcccgggct
     3481 gcgctggagg cgaccaccgg aaggggcgag gacgctgcag aactcgcatc ccggcgggag
     3541 cgccttcttg cggaacacag ccgctaccag gagttgcaag ccctccggga gagcctgctc
     3601 cgcgaaaagg aagatgtgca ggccaggcac aaggccctcc aggaacagcg gcagattctc
     3661 ggaacgggac gctgtcccct tttgggcgag acatgccgga atctcgcagg ccggggatcg
     3721 gatgccgagg gcgagatcgg tcgtcttctg gaggagttgt ccctcgggac caggcaggcc
     3781 gccgagcggc tcaacgaggt gacggccgag atggcccgat tcgagaaggc gggagggcag
     3841 ctcggggccg tcacccaggc catcgccgag gcggagaaac gggaaacgga gcgcagggag
     3901 cgtgaggtga gactgcggga gatcgagacc gtctgcaggc aggccgaaga gcgctttgat
     3961 gcggtcgcct cgtcgcttgc gaacatcccc ggattccacc ccctcaaaca agccattccc
     4021 gaacggatat ggtcctcgcg tgggcaggac agacaggtct ttctggaaca tctccgcaag
     4081 gcacagaccg cctggcaggc cgggtggcgg ctcgagcggg agcagctgac ggcccttgtc
     4141 ggggagcgtg ccagcagaca gaaacggtgt gaggacgggg agtccgccgt acgggcggac
     4201 cgcgcagatc tcgaacgcgc ccgggatgcc taccggagcg tgtggcgcag gatacgggac
     4261 gaggaggggg ttggccagtt cgtccagacg ctgccgccgg aacccgatct cgcagccctc
     4321 acggttcttg aggatgaact tgatgcacgg cgcaccccgc tcgccagcct gcgggaggaa
     4381 gccggggcca gacgcgggcg cctcgccggt gaagagggtt tgcatgaccg tcttggacag
     4441 cttgaagccc ggagaaaaga ggaggacggg atcctccggg aacgggagac cggattcgat
     4501 gcggccgcga gcgtgctggc gctctcctgt cgggccatcg gtgaacctgg tcccgctgct
     4561 cccgctgtcg aaaacctgga gtccctgtct gcactcacca gggagatagg cctcctgcgg
     4621 gaggatgtct cgaatgccat tggtcagacc agccaggcgc tggagggttt gcgtcaggag
     4681 caggaaaatc tgaggcgtct ccgtgaaagt ctggcttcca cgcttgagca tctcggcgaa
     4741 gcccgacgca gacttgatct tgtgcagcgg atcagggggc tgctcaagga tcttggacca
     4801 tggatctcga aatacgcgct gcctctgatt gcccgggatg caacccgtca cttccgccgg
     4861 atcaccggac gcagcgaacg cattctctgg actcatacag ccaaggacga gtatctcgtc
     4921 tccctcgctg aaagcgagga ggacagcggg gtacgcagtt ttgtccagct ctcgggaggc
     4981 gagcaggttg cagtcgctct cgccgtacgg gccgccctgg tcgagtccct ctcgtcatcg
     5041 ggattcgtga tttttgacga gccgacgatc aatctcgata ttgaatcccg tcgcaatctg
     5101 gccggggcct tcaaggccgt cttcggcaag atcgagcagg ccttcgtaat cacgcacgat
     5161 gacagctttg aagccatggc ggacaacgag atccggtttg attgagatcc ggtcggtatg
     5221 gtgacagcgg ccccgggccg gcgtgtgcga ttggaacgcc ggttctcatc gggagatcac
     5281 ggcagagtcc ccgtactccc gatttttgat gagggctctt gattgtcccc gtgctccatg
     5341 gcatactgaa ggggtcagca cggagggagt agtttgatgc cgggagcaca tgcgatcatc
     5401 cacggggagg gaaggattcc cgagagcgag taccgcttct tttttgtcac caatgtcgcc
     5461 tttctcttcg gttttgtcag ccacgccatt tttctgacgg ttttttcctt tctgggtgtc
     5521 ccggagatgg tgcttcacaa tattgccagt tgcttcgttt tttttatcgc catccttgcc
     5581 aaccagcagc ggcggtatcg tttggcgatg tcactcgcct acttcgaggt gatgatccac
     5641 gctgccatgg ccgtcgtctt catcgggcag gacagcggat tccagtatta cgtctttgtg
     5701 gttgttcccc tgatttttta catatcggtt gtgccggcct gggtccgctt catgatggcc
     5761 gtcgtggctt ttcttgtcta cgcgttcatc tcctattttc tccgcggtga gccggtctac
     5821 agtgtggatc cctgggtgct gcggttcatc agggggggca acatcgccgg agtggtcgcg
     5881 ggcttttcgt ttatcgggta ctggtacgga cggatcaata tgatcgccga ggccgcgctc
     5941 tttgccgaac ggaacaagtt gcgtgaaaaa aacgagcgga tgcagagcga gctcgagatg
     6001 gcccggagga tccagctcca gctcgtaccg gctgtcggcc cggtcaaggg tctcgcgttt
     6061 gaataccgtc cgatgacgat cattggtggt gacttttatg attttgtgac gtttgtgggc
     6121 aggcctcttg aaatcggcat ttttatcagt gatgtctccg gacacggtgt tccggccgcc
     6181 ctgattgcct cgatgctcaa gagcacgctc ctgcagtcag tcgatattct cgatgacccg
     6241 ccggcactga tgacgcggct caatgatacc ctcgtctcga agacaaatga caatttt
//
LOCUS       DAOSHE010000079         6296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_10893_length_6296_cov_4.672969, whole genome shotgun sequence.
ACCESSION   DAOSHE010000079 DAOSHE010000000
VERSION     DAOSHE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10893_length_6296_cov_4.672969"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLD82_03470"
     CDS             <1..488
                     /locus_tag="PLD82_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64475.1"
                     /translation="GAFMFPIGLFCMTKAARQGQAGDVFGVALVAAAIKSVNLFFPHL
                     TPVSTLHPMIAILLQSAFGYAFIQGLKKSYSPFLLVPAAVVGWRIAFIGVQVLFAGIQ
                     GQTAGVNLSAMFAGSLIDIALVSLLLVSKPSTQPRQSVSFASLALVLAISVQGIITAI
                     A"
     gene            612..2609
                     /locus_tag="PLD82_03475"
     CDS             612..2609
                     /locus_tag="PLD82_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG64476.1"
                     /translation="MFRRLFLISIISLLFTSSIIGTQPEPVVLLGSIEVTSRPWSDTS
                     IHVIGSNTLQNKANNGLVDVLQLVPGITMSVSGGRNEANFSMRGFDVKHAPVFVDGIQ
                     VTAPYDGYTDVGRHLLWGLASVEVVRGYVPLSLGPGTLAGAINCVSARPEKEYQARME
                     SGLRLDNDSTRSLWWTGANASGKRDAWHFKTDFFTEERDFVPVPGDFTPTATEDGGKR
                     DNSFSGNSLVALELGWTPDQESEFILRVSIQRGDKGIPVYAGSNTSLNKPRYWQMPEW
                     DKDSISMIGRTAITDSILVKTRFWYDSFDNTVKSYDNNAYLAMTKPSSFASIYNDKNF
                     GAACEMAIDLSSNWVLQPFLLYRHDQHQGHNVGEPEVTQEDDTVSIGVEQSYSISRDL
                     VFRAGIAWSQANPGTVEDLVKSGTNYSILPFATSPRVAWDLQAGLWWKAGETATLHTS
                     VAKRTRFPTLKDRYSYKLGKAWANPGLMPEDSVQVEAGASMHLAPWLSFNGTIFVSET
                     EKYIQVVPVTIGTETRLQSCNVGRVRIPGLELAIEGKISSVLNWNLTASWINPDNLDS
                     TADIPDIPKVQCSAIVGVQALDNLSFDLEWQYSGSRTDMNDQSVTLDGYHLAGLATHW
                     TISKLARLSLGVANIFDTLSYASEGYPLAGREIWVKADFTY"
     gene            2740..2970
                     /locus_tag="PLD82_03480"
     CDS             2740..2970
                     /locus_tag="PLD82_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64477.1"
                     /translation="MITRTLTVGTEPISAHRSNDWRIEMEETYFRNNTGDHSLTAQGI
                     TLEAGKTYSIRGVVDAFGDKKVVAIFQASLVN"
     gene            complement(3152..3286)
                     /locus_tag="PLD82_03485"
     CDS             complement(3152..3286)
                     /locus_tag="PLD82_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64478.1"
                     /translation="MEREFGVLETIDDNYPKYVVSLDGINRSQNGILHQNIRDFLLGK
                     "
     gene            3589..3966
                     /locus_tag="PLD82_03490"
     CDS             3589..3966
                     /locus_tag="PLD82_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64479.1"
                     /translation="MENRIAITISSGRISPVFDVARTACVFVGKDDNPVATIELPSSL
                     AQRADVLAKHGITRLVCGAISRQAHILVENTGIRVIGFVAGEASEILAAARSNRLPDR
                     HYAMPGCRNCCRRRHERRGCGQP"
     gene            4019..4369
                     /locus_tag="PLD82_03495"
     CDS             4019..4369
                     /locus_tag="PLD82_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG64480.1"
                     /translation="MPRGDRTGPQGFGSRTGRGLGYCSGYNQPGYVTGGGAGFGGGYG
                     FGGGAGFGGGRRGGRGRGWCAYYAAPAAPVYQPAAERDVLESRLDTLQRETAWIKSRL
                     DSMQNNEGTSGDQQ"
     gene            4366..4728
                     /locus_tag="PLD82_03500"
     CDS             4366..4728
                     /locus_tag="PLD82_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG64481.1"
                     /translation="MKIAISARSASLESEFDSRFGRAPLFVIYDTDTREFASCSNTQN
                     LQAAQGAGIQTAQNVANAGAQAVVSGHCGPKAFTVLTAAGVTVYQAKAATVQDVIRQF
                     EQGGLVPMSSADVDGHWV"
     gene            4725..5561
                     /locus_tag="PLD82_03505"
     CDS             4725..5561
                     /locus_tag="PLD82_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64482.1"
                     /translation="MKLAIASGKGGTGKTTLAVALATLLPGCRLLDADVEEPNARLFV
                     KGEELESRTVTMPIPVVNPDTCTGCGACVRFCRFNALALAGRRIFVFEELCHGCGGCS
                     LVCPTRAIHEKPLPIGALSTILNDTWSLVEGRLNVGRMAAPLVIRRLLAQANKTGETI
                     IDCPPGTSCNMVTAVRDADYTILVTEATAFGLQDLSLAVNTMRKLKKRFGVIINRADL
                     GDDRVRRYCADESVKIHLEIPFSREIAQAIANGKTMLDGAPEFEAQLRSVLAQTGMGG
                     IA"
     gene            5558..>6296
                     /locus_tag="PLD82_03510"
     CDS             5558..>6296
                     /locus_tag="PLD82_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG64483.1"
                     /translation="MKELVILSGKGGTGKTSMAGACIALAHRDTGKVVLADADVDAAD
                     LFLLTQPVPIEEEDFTSGVEAHINPERCTGCGRCFSVCRFDAIRILDNGKAQVTETGC
                     EGCAVCAHVCPVHAIDLVDRHCGVTWTSDTRFGKMFHAKLDPAGENSGKLVTLVRQWA
                     KEYAKSAGAEWLIVDGPPGIGCPVIASITDADAILTIAEPGLSSKHDLIRLLELARHF
                     GLPVMLIVNKWDIDPDITVNIENEARQM"
BASE COUNT         1487 a         1716 c         1681 g         1412 t
ORIGIN      
        1 cgggcgcctt catgttcccc atcggtctct tctgcatgac gaaagcagcc cgccagggcc
       61 aggccggcga cgtcttcggg gttgccctgg tcgccgccgc gatcaaatcg gtcaatctct
      121 tcttccccca tctgaccccg gtttcaacgc ttcacccgat gattgcgatt ctcctccagt
      181 cggccttcgg gtatgccttt atccaggggc tcaagaagag ctactcacca tttctcctcg
      241 ttcctgcggc tgttgtcggt tggagaatcg cgtttatcgg agtacaggtt ctcttcgctg
      301 gaatccaggg acaaacagca ggtgtcaatc tgtcggccat gtttgcgggc agcctgattg
      361 atattgcact tgtctcactc ctgcttgtca gcaaaccgtc aacacaaccc agacagagtg
      421 tcagtttcgc cagtctggcc cttgtgctgg caatcagtgt gcagggcatc atcacggcaa
      481 tcgcttaagc atacccaccg tgtatatcgg gaaggacgga aaaaaggaac tccgaaagcc
      541 atgggacaac ccttgtcatg gcattccttc ccgcacggta tccaatacta caagggaaag
      601 gtagttccag aatgtttcgt cgtctgtttc tcatttcaat tatttcactc ctcttcacat
      661 cgtccatcat tggtacacag cctgaaccag ttgtcctgct cggatcgata gaagtcacct
      721 cccgaccgtg gagcgatact tcgatccacg tcatcggaag caatacactc caaaacaaag
      781 ccaataacgg gctggtcgat gtcctgcagc ttgtaccggg catcacgatg agcgtctccg
      841 gtggacggaa tgaagccaat ttcagtatgc gcggttttga tgtcaaacac gccccggttt
      901 tcgttgacgg aatccaggtt accgcaccct atgacggata caccgatgtc ggccggcatc
      961 ttctctgggg cttggcctcc gtcgaagttg ttcgcggcta tgttccgctc agcctcgggc
     1021 cgggtactct ggccggagcc atcaattgcg tctccgcaag acccgaaaag gaataccagg
     1081 ctcgaatgga aagcgggctt cgccttgaca atgactctac acgaagtctc tggtggacag
     1141 gtgccaatgc ttcaggaaaa cgggacgcgt ggcactttaa aacggatttt tttacggaag
     1201 aacgcgattt cgtccctgta ccaggtgatt ttacaccgac agcgacggag gacggtggaa
     1261 agcgggataa ctcgttctca ggaaatagtc ttgttgcgct tgaacttgga tggacccctg
     1321 accaggagag tgaattcatt cttcgggtct ccatccagcg aggggacaag ggaatcccgg
     1381 tctacgcagg cagcaacaca agtcttaaca aaccccgtta ctggcagatg cccgaatggg
     1441 acaaggacag catctccatg atcggtagga cagccatcac ggattcaatc ctggtcaaaa
     1501 cgcgattctg gtacgacagc tttgacaaca cggtcaagag ttacgataac aatgcatacc
     1561 tcgccatgac gaagccctca tcgtttgcaa gcatctataa cgacaagaat ttcggtgccg
     1621 cctgcgaaat ggcaattgat ctttccagca actgggtttt gcagcccttt cttttgtacc
     1681 gacatgatca gcatcagggc cataatgtcg gagaacctga ggtgacccag gaagacgaca
     1741 ccgtctcaat cggagtggaa cagtcgtact ccatatcacg cgatctcgta ttccgggcgg
     1801 gcatagcctg gagtcaggca aatccgggaa ccgtggagga tcttgtcaaa tccggtacaa
     1861 attactcaat tcttcctttc gctacctcac cccgggttgc atgggacctt caggcaggcc
     1921 tttggtggaa agcgggagag acagcaacac tgcatacctc cgtcgccaaa cggacacgct
     1981 tccccacact caaggaccgc tattcataca aactgggaaa agcctgggcc aatcccggtc
     2041 tgatgcccga agattctgta caggttgaag cgggtgcatc catgcatctc gctccatggc
     2101 tctccttcaa cggaaccatc ttcgtcagtg aaaccgaaaa atacattcaa gttgtcccgg
     2161 tcaccatcgg cactgaaaca cgccttcagt catgcaacgt cggtcgtgtg cgaattccgg
     2221 ggctggagct tgccattgag ggaaaaatct cctccgtcct gaactggaat ttgacagcga
     2281 gctggattaa tccggacaat ctcgattcga ctgcggatat tcctgatatc cccaaggtgc
     2341 aatgttctgc gattgtcggc gtccaggcgt tggataatct ttcgtttgat ctcgaatggc
     2401 agtattctgg ctcccggacc gatatgaacg atcagagcgt gacacttgat ggatatcatc
     2461 ttgcaggctt ggcaacccat tggacaatca gcaagctggc acggctttcg ttgggcgtgg
     2521 caaacatctt tgacaccctg tcctacgcca gtgagggata cccgctcgcc ggacgtgaga
     2581 tttgggtaaa agcggacttt acgtattgat acccaaaccc gggaaagcac tcgcttcccc
     2641 gggtttttat gctctccggg agagcgtaac cggttcgcct ggctatcggg attctatctg
     2701 attgacagcc cgaccatcag tatcgaaaac cagggaaaaa tgatcacccg aaccctgact
     2761 gtgggcactg aaccgatctc tgcccatcgt tccaatgact ggcgcatcga gatggaagag
     2821 acctatttca gaaacaacac gggagatcac tcgttgacgg cccaggggat tacgctcgag
     2881 gccggcaaaa cctacagcat tcggggtgtg gttgatgcct ttggcgacaa aaaggtcgtc
     2941 gccatcttcc aggccagtct ggtgaactga gacagccctt cccattttcg gtgtgtccat
     3001 ccctgcggtc tctcctgatc gaaaccgcag ggtcctgtgc aaccgcgtca aacggatgcc
     3061 gttcaaatcc cggccaattc gacattgtca cagaaatccg cgaatataat ggtgttttcc
     3121 cctgtgaaat cacggactat cgcatcggat gctatttccc cagcagaaaa tcccggatgt
     3181 tttggtgcag tatcccgttt tggctgcgat tgatcccgtc cagcgagaca acatacttcg
     3241 gataattatc gtcgatcgtt tcgagcacgc cgaactcacg ctccatcgtc tcgtcgccac
     3301 aaaccatatc gaaaaaacaa ggctggattc ctatttcctt tcgttatatc gaaaagaacg
     3361 tcctacgccc cggtcagggg tgagcaacac ctcttgcctg cctgaaaacc ccgtacaggg
     3421 gggggggcat tgcaagaatg caacacgcgc attgcgtctt tgcgatcagt atctgtccga
     3481 caggacacca gacaagatcg cttgattgac aaatcacgac attataaaca gttacaagat
     3541 gctgcggaat tggcacgcaa gatgcaatat aaggcagcag gggtcatcat ggaaaaccgt
     3601 atcgccatca ccatctcgtc aggccggatc tcaccggtgt ttgatgtcgc ccgcacagct
     3661 tgtgtttttg tcgggaaaga tgataacccg gttgctacca tcgagctgcc atcaagcctg
     3721 gcacagagag ccgatgttct tgccaaacac gggataaccc ggcttgtctg tggagccata
     3781 tcccgccagg cacacatcct cgtcgagaat accggaatca gggtgattgg ctttgtggcc
     3841 ggcgaggcga gtgaaatcct tgctgcagcc cgaagcaatc ggcttcccga cagacactat
     3901 gccatgccgg gatgcagaaa ttgttgccga agacggcatg aaaggcgcgg ttgtggtcaa
     3961 ccataatgct cccatctcaa caaccatccg gatatccgga tgaggcaagg aggaaaatat
     4021 gccacgaggt gacagaacag ggccacaggg gtttggctcc cggacaggac ggggactggg
     4081 gtattgctca ggctataacc agccaggata cgtaacaggt ggtggtgccg gtttcggcgg
     4141 tggatacgga tttggaggag gagccggctt tggaggggga cgccgtggcg ggcgtggtcg
     4201 cggttggtgc gcatattacg cagcgcctgc agcaccggtg tatcagcccg ctgctgaacg
     4261 cgatgtcctc gagtccagac tcgacacact ccaaagagaa accgcctgga tcaaatcccg
     4321 gctcgattct atgcagaaca atgaaggaac aagcggagac caacaatgaa gattgccatc
     4381 agtgcccgga gcgcatcact tgagagtgaa tttgactccc gttttggaag agccccgctt
     4441 tttgtgatct acgatacgga taccagggaa ttcgcatcat gcagtaacac acaaaatctt
     4501 caagccgcac agggtgccgg gattcagaca gcacagaatg ttgccaatgc cggcgcccaa
     4561 gcggttgtga gcggacattg cggcccaaaa gcctttaccg tgctcactgc agccggagtt
     4621 acggtgtatc aagccaaagc ggcaacagtt caggatgtga ttcgtcagtt tgagcagggg
     4681 ggactcgtcc ccatgagttc cgctgatgtc gatgggcact gggtatgaag ctggcaattg
     4741 cttcaggcaa gggcggcacg ggaaaaacaa cgctcgccgt tgctctcgcc actctcctcc
     4801 cgggatgccg gcttcttgac gctgatgttg aagaaccgaa tgcccgtctc tttgtcaagg
     4861 gtgaggagct tgagagccgg accgtaacca tgcccatccc ggttgtcaac ccggatacct
     4921 gcactggctg tggcgcctgc gtccgcttct gtcggttcaa tgcgctggca ctcgcgggca
     4981 gaaggatttt tgtgtttgag gaactctgcc acggttgcgg aggatgctca ctcgtctgtc
     5041 cgacacgcgc catccacgaa aagccgctgc ccatcggggc actgagcacg atcctgaatg
     5101 acacatggtc gcttgtcgaa ggaagactga atgtcggacg aatggcggct ccactcgtaa
     5161 tccgccgcct gctcgcccag gcaaacaaga ccggtgaaac aatcattgac tgcccacctg
     5221 gaacctcatg caatatggtc acagcagtcc gtgatgctga ctatacaatt cttgttaccg
     5281 aagcaacagc gttcggcttg caggacctct ctctcgcagt caatacaatg cgaaagctga
     5341 aaaagcgttt cggggtaatc attaacaggg cggacctggg ggatgaccgg gttcgacggt
     5401 attgtgctga cgaatcagtc aagattcatc ttgaaatccc tttctcccgg gagatcgccc
     5461 aggccattgc caacgggaaa accatgctcg atggtgctcc tgagtttgaa gcacagctcc
     5521 ggagcgtcct ggcgcaaacc ggaatgggag ggattgcatg aaggaactcg tcattctttc
     5581 aggaaaaggc ggaaccggaa agaccagtat ggccggggcc tgtattgcac tggcacaccg
     5641 ggataccgga aaggttgtgc tggctgatgc agatgtggat gcagccgatc tctttctcct
     5701 gacacagcct gttcctattg aagaggaaga tttcacatcc ggcgtggaag cgcacatcaa
     5761 tccggaacgc tgtaccggct gcggtcgctg tttttcagtg tgtcgtttcg acgccatccg
     5821 tatccttgat aatgggaaag cgcaagtcac tgaaaccgga tgcgaaggat gcgctgtctg
     5881 tgcccatgtc tgtccggttc atgcaattga tcttgtcgat cgacattgcg gagtgacctg
     5941 gacttcggac acccgcttcg gaaaaatgtt ccatgccaaa cttgaccctg ccggtgaaaa
     6001 ttcaggcaag cttgtcacat tggtccgtca atgggccaag gagtacgcaa aaagtgccgg
     6061 agctgaatgg ctgattgtcg acgggccgcc cggaattggc tgccccgtga ttgcatcgat
     6121 aaccgatgct gatgccattc tgacaattgc cgaacccgga ctctcctcaa agcatgatct
     6181 gatccggctg cttgagcttg cccgacattt cgggcttccg gtaatgctca tcgtcaataa
     6241 atgggacatt gatcccgata ttaccgtgaa tattgaaaac gaagcccgcc agatgg
//
LOCUS       DAOSHE010000080         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11057_length_6234_cov_3.375303, whole genome shotgun sequence.
ACCESSION   DAOSHE010000080 DAOSHE010000000
VERSION     DAOSHE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11057_length_6234_cov_3.375303"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLD82_03515"
     CDS             <1..235
                     /locus_tag="PLD82_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOG64484.1"
                     /translation="ERYVELGIDGFEAWHPHVDKRLKRTLKRLARRHGLVLTGGSDSH
                     GSWGNRPRVGGVTVGYSYVYELLDRMVLRRGRL"
     gene            381..848
                     /locus_tag="PLD82_03520"
     CDS             381..848
                     /locus_tag="PLD82_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG64485.1"
                     /translation="MAGKNDQINSVIGEGSVFVGKFYVHGSLQIDGKFEGEIRTEDHL
                     VIGETGKVKTDIVANRVTVGGTLVGNIEAVEEVRLLETGRVLGNIVTPQLLVEGGVVS
                     QGVVTITGGQKKDVRKIILDSYASGPVLPDLKGHKSIEGKEEAPAPTGRRGRS"
     gene            950..2068
                     /gene="alr"
                     /locus_tag="PLD82_03525"
     CDS             950..2068
                     /gene="alr"
                     /locus_tag="PLD82_03525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG64486.1"
                     /translation="MSVASRTSRCWSEVSLSAIGKNIRAIRRLAGGASLYAVIKADAY
                     GHGVLELARLLENAGVDTFCVATLGEAVQLREAGYTRQTILLLGSFYADEVGDLFEYA
                     VTPTIPDITHLGILSRHARRKPNPIDAHLKVDTGMNRYGIPFDELTERAQEIFRTPGV
                     NITALYSHLAASALPGDQGTLDQIAQFTRAVDYLEVSNLWFGRKHICNSGGVLYYPQA
                     HLDAVRPGLILYGYYPGAIDRDRVALSPSLSFHARVTAVKKLFPGQSLGYDRTYTAKK
                     EMTIALLSVGYADGYPIRFSNKGQVLLGRERCSVVGKVCMDTTMIHVPSGRVKPGDSV
                     LLWGGREHPLETVAAGAGTIPYELVCQISRRVPRLHVD"
     gene            2058..3056
                     /locus_tag="PLD82_03530"
     CDS             2058..3056
                     /locus_tag="PLD82_03530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG64487.1"
                     /translation="MSTEKPMISVIIPHRTSETIDATLLALESAARHARESGHPEAAR
                     YEVLAVSGNQPSVQRNTAVRQASGEFVYFLDNDSVPATDCLMRVANFFSIHSDAAILG
                     GPSLTPESDNTSQKAFGHVLGSFAGTAFIRARYSSFGVLRETTDRELILCNLAMRRQT
                     FLDLGGFDERLYPNEENALMDKALQEGWTLWHDPLLRVWRSQRTNFRAFIRQLFGYGR
                     GRAEQMRLAPLVSNIVPFIFMLFPLAMLLLVPSLMILPDLVWCWLTYPLATFLASIPS
                     LAAGPVVAGKAWLAFFLCHLCYGCGLWAGLFLPLRSRTVVASVQCRKIAAPKAGRR"
     gene            3056..4741
                     /locus_tag="PLD82_03535"
     CDS             3056..4741
                     /locus_tag="PLD82_03535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG64488.1"
                     /translation="MSDQRARPLILQSDMTVMLEVAHPEFDGLRSTLGSFADLEKSPE
                     HIHTYRITPLSIWNAASAGVPLDVIREFLVENSKYEIPQNLLFQVEELYSRYGRLVLR
                     LDEDDPARIILESDDPLILTEAVTSKRIAPFITGTVSERAVTINPAWRGFIKKEFIDL
                     GYPVRDEAGYRDGAGFSFSLREQAATGKPFGLREYQIRSVERFWEAGKNTGGCGVIVL
                     PCGAGKTIVGMAVMHRYQCQTLILTTNIVALRQWKNELLDKTDIPMEMIGEYSGDSKE
                     VRPVTIATYNILTWRRDKESDFEHFGLFMQNDWGLIIYDEVHLLPAPVFRVTAELQSR
                     RRLGLTATLVREDGLEKDVFSLIGPKKYDVPWKDLEKKDWIAKAVCHEIRVEMARDLR
                     MTYVASDEREKFRLASENPAKLDVIEKLLVRHADENVLIIGQYLEQLDIVARTFSLPV
                     ITGATPVPQREQLYRDFREGRVPVLVVSKVANFAIDLPDASVAIQISGTFGSRQEEAQ
                     RLGRILRPKGGSNEAVFYTVITRASKEQQFASNRQLFLTEQGYSYTIENWQPT"
     gene            4935..6140
                     /locus_tag="PLD82_03540"
     CDS             4935..6140
                     /locus_tag="PLD82_03540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64489.1"
                     /translation="MQRLIQSSVFFSALVLWATPSVAAGVRVTDGFDRSGQKFQIVAV
                     SQESGPVEIRLTAPTIRNLQAISGIPFTTQLQAGRTHRIQFSIPDTYAQSYITWQLDW
                     RRVGGTSWSRASQHSFAGTFSPASAPTVTDGFDPARRQFRVMVQNRMNSAIEFRVSAD
                     AGMRNLSARGPNPWTITVPANGQGTVTWNVTDPGQQSYITWRAEFRPQGGTQWLAASQ
                     HTFAGTMTPGSSTSNQRDSYFAGGLYLIPYGRNATYTMSNGPNGYGWHAGKNAYDWPL
                     PMNTMVLCARSGTVVSLKKDSNRGGASQSYQNDANYIQIRHDDGTIANYLHFAYNSIL
                     VRVGQRVTVGEELGRSGNTGWTTQPHLHFHVSNAAGNVTYRLRFLTRNGPVDPASGTR
                     MTGYYPWMP"
BASE COUNT         1221 a         1762 c         1898 g         1353 t
ORIGIN      
        1 tgagcgctat gttgaactgg ggatcgacgg attcgaggcc tggcatcccc atgtcgacaa
       61 gagactgaaa cgcacgctca agcggctggc tcgccggcat ggcctggttc tgaccggggg
      121 gagcgattcg cacgggagtt ggggaaaccg gcccagggtg ggaggagtca ccgtgggcta
      181 ctcatacgtg tatgaacttc ttgaccggat ggtccttcgc agggggcgct tgtgaagcgg
      241 atccctccac cggatcgggt ccagtccgga cagtcttgcc atggaagcct tgtgtaaaaa
      301 cgcttgaaaa ttggcagttc ccgggatatc ttggacgggt atgtgtgttt ttgtaacgcg
      361 ccccaaggag aagacaggct atggccggga agaatgacca gattaacagc gtaatcggcg
      421 aaggctcggt attcgtcggt aaattttacg tgcacggatc gttgcagatt gatggaaagt
      481 tcgaagggga gattcgcacc gaggatcacc ttgtgattgg cgagaccggt aaggtcaaga
      541 cggatatcgt ggcaaaccgt gtcacggtcg gtggcacctt ggtcgggaat atcgaggcgg
      601 tcgaggaagt tcgtcttttg gagaccgggc gtgttcttgg aaatattgtt actccccagc
      661 tccttgtcga gggtggtgtg gtgtcgcagg gcgtcgtgac catcacaggc gggcagaaaa
      721 aagacgttcg caagatcatc cttgactcct atgccagcgg tccggttctg ccggatctca
      781 agggccacaa gagtatcgag ggcaaggaag aggctccggc cccgacgggg cgcaggggtc
      841 gctcttgagc gtaccgggga cggtatgctg attgtgttgt accgctaacg ctcccttcga
      901 gggggcgttc tgtattgata ggggagggtg ccccgggagg cgaagcgcag tgagtgtggc
      961 aagccggaca agccgttgct ggagcgaggt cagtctttca gcgatcggaa agaatattcg
     1021 tgccatccgg cgtcttgcgg gtggggcttc cctgtatgcc gtgatcaagg cggatgccta
     1081 tgggcatggt gttctcgagc tggcccggct cctggagaat gccggagtcg ataccttttg
     1141 tgtcgcaacc ctcggcgagg cagtccagtt gcgcgaggcc ggatataccc ggcagaccat
     1201 tctcctcctt ggttcttttt acgctgatga agtcggcgat ctttttgaat atgctgttac
     1261 acccacgatc cccgatatca cccatctcgg gatactctcc cgccatgcca ggcgaaaacc
     1321 gaacccgatt gatgcccatc tcaaggtgga tacggggatg aaccgctatg ggattccgtt
     1381 cgacgaattg actgagcgtg cccaggagat ttttcgcacc ccgggcgtca atatcacggc
     1441 actctattcc caccttgcgg catcggctct tcccggtgat cagggtacac tggaccagat
     1501 tgcgcagttt acccgtgcag tcgattacct tgaagtatcg aatctctggt tcgggcgcaa
     1561 gcatatctgc aacagcggcg gggtcctgta ttacccccag gcgcatctcg atgcggtccg
     1621 tccagggctg atcctctacg ggtattaccc gggggcgatt gaccgggacc gggttgcgct
     1681 ctcgccctca ctgagttttc atgcccgcgt gaccgcggtc aaaaaactct ttcccggtca
     1741 atccctggga tacgacagga cctataccgc aaaaaaagag atgacgatcg cactcctgtc
     1801 ggtcgggtac gctgacggat atccgatccg cttctcaaac aaggggcagg ttcttctggg
     1861 tcgggaacgg tgttcggtgg tgggcaaggt ctgcatggat acgaccatga tccatgttcc
     1921 ttcgggacgg gtaaaacccg gggacagtgt gcttctctgg ggcggccgtg aacatcccct
     1981 ggagacggtt gcggctggtg cgggaaccat cccgtacgaa ctggtttgcc agatctcacg
     2041 aagggtgcca cgcttgcatg tcgactgaaa aacccatgat ctccgtcatc attcctcaca
     2101 ggacaagtga gaccattgat gccaccctgc tggccctgga gtcagccgcc cggcacgcgc
     2161 gtgaatccgg ccatccggag gcggcccgtt acgaggtgct tgccgtgagc gggaatcagc
     2221 catccgtcca gagaaacacc gccgtccgtc aggcctccgg ggaatttgtc tattttctcg
     2281 acaatgattc cgttcccgct acggactgcc tgatgcgggt ggccaatttc ttctcgatcc
     2341 attccgatgc agcgattctt ggcggtccct cgctgacacc cgaatccgac aatacctcgc
     2401 aaaaggcgtt cggtcatgtc ctggggagtt ttgccgggac cgctttcatc cgtgcacggt
     2461 attcctcgtt tggtgtgctg cgcgagacca ccgatcggga gctgatcctc tgcaatctgg
     2521 ccatgcgcag acagaccttt cttgatctgg gcgggtttga cgagcggctc tatcccaatg
     2581 aggagaatgc cctgatggac aaggccctcc aggaaggctg gacactctgg catgatcccc
     2641 tgctcagggt ctggcgcagt caacggacca atttccgcgc cttcatccgg cagctcttcg
     2701 gatatggaag gggacgggcc gaacagatgc ggctggcccc ccttgtgtcc aacatcgttc
     2761 ccttcatctt catgctcttt ccgctagcga tgcttcttct cgtgccaagc ctcatgatcc
     2821 tgccggatct ggtctggtgt tggctgacct atccgttggc aacctttctg gcgagtattc
     2881 cctccctggc ggcagggccg gtggtggcag ggaaggcctg gctggcattt ttcctctgcc
     2941 atctctgtta cgggtgcggt ctctgggcgg gactctttct tccgttgcgg agccggaccg
     3001 tggtggccag tgtccagtgc cgaaagatcg cagcaccgaa agccgggcgc cgctgatgag
     3061 tgatcaacga gcccgccccc tgattctgca gtccgacatg accgtgatgc tcgaggtggc
     3121 gcatcccgag tttgatggtc ttcgttcgac cctgggatcc tttgccgatc ttgaaaaatc
     3181 tccggaacac atccatacct accggatcac tcctctctcg atctggaacg cggcatccgc
     3241 cggtgttccc ctggacgtca tccgggaatt tctggttgaa aacagcaaat acgagattcc
     3301 gcaaaacctg ctcttccagg tcgaggaact ctactcccgg tatggccggc ttgtcctgcg
     3361 gctggacgag gatgatccgg cccggatcat cctcgagagc gatgatcccc tgatcctgac
     3421 agaggctgtc acgagcaaga ggatcgcccc cttcatcacc gggactgtca gtgaacgggc
     3481 ggtcacgatc aaccccgcct ggcgcgggtt catcaaaaag gaattcatcg acctcggata
     3541 cccggtgcgg gacgaggccg ggtacaggga cggggccggg ttctccttca gtctccgcga
     3601 gcaggccgcg accggcaagc ccttcgggtt gcgggagtac cagatccgtt ccgttgagcg
     3661 tttttgggaa gccggaaaaa acacgggcgg gtgcggcgtg atcgtgctcc cctgcggggc
     3721 ggggaagacc atcgtcggga tggccgtgat gcaccgctac cagtgccaga cactgatttt
     3781 gacgaccaat attgtcgcac tccggcaatg gaaaaacgaa ctgctcgaca agaccgacat
     3841 cccgatggag atgatcggcg agtattccgg agacagcaag gaagtccggc cagtgacgat
     3901 tgcaacgtac aacattctga cctggcgccg cgacaaggag agcgatttcg agcattttgg
     3961 gctttttatg cagaatgact ggggcctgat catctacgat gaggtgcatc tccttcctgc
     4021 tcccgtcttc cgggtgacgg ccgagctcca gtcacggcgc cggcttggct tgacggccac
     4081 cctggtccgg gaggatggtc tcgaaaagga tgtcttctcc ctgatcggtc cgaaaaaata
     4141 cgatgttccc tggaaggatc ttgagaagaa ggactggatt gccaaagcgg tctgccacga
     4201 gatccgggtc gagatggcca gggacctcag gatgacctat gttgccagcg atgaacgtga
     4261 aaaattccgg ctggcctccg agaatccggc caaactggac gtgatcgaaa aactcctggt
     4321 ccggcatgcc gacgagaatg tcttgatcat cgggcaatac cttgagcagc tcgatattgt
     4381 ggcaaggacc ttttcgctcc cggtcatcac gggagccact ccggttccac agcgggaaca
     4441 attgtaccgg gattttaggg agggccgggt ccccgtcctg gttgtctcca aggttgccaa
     4501 ttttgcgatc gatttgccgg acgcttcggt tgccatccag atttcgggga ccttcggctc
     4561 ccgccaggag gaggcccagc gcttggggcg gatcctccgg cccaagggcg gctccaatga
     4621 ggcggtgttc tacaccgtga tcacgcgtgc cagcaaggaa cagcagtttg cctccaaccg
     4681 ccagctcttt ttgaccgagc agggatacag ctacacgatc gagaactggc aaccgacctg
     4741 atccatgagt ctgtgcaaga agcccgcact gtattgcagt tctgtgatgc gactccggga
     4801 taccgatgtt ctcatgggac tcccggagtc gcatcacgaa ttcatgattc tgtgccgggt
     4861 ctcccggatt gttttttccg gtggctgtgc taacctttat gggacagtac gtggagatca
     4921 cccgggaggc atccatgcag agactgatac agagctctgt gttttttagc gcacttgtgt
     4981 tgtgggcgac accgtccgtg gcggccgggg tcagagtgac ggacgggttt gaccgttcgg
     5041 ggcagaaatt ccagatcgtt gccgtcagtc aggagagcgg accggtcgag atccggctga
     5101 cggctcccac catcaggaat ctgcaggcca tctccgggat cccgtttacg acccagctcc
     5161 aggctggcag gacacaccgg atccagtttt ccataccgga cacgtacgcc cagtcctaca
     5221 tcacctggca gcttgactgg cgacgggtcg ggggcacttc ttggagccgg gccagtcagc
     5281 attcgtttgc aggcactttt tcacctgcgt ccgctccgac tgtcacggac gggtttgatc
     5341 cagcccggag acagttccgc gtgatggtcc aaaacaggat gaattctgcc atcgagtttc
     5401 gtgtcagtgc tgacgcgggg atgcgcaacc tttcggcgcg ggggcccaac ccctggacca
     5461 ttaccgtgcc tgccaacggg cagggtacgg tgacctggaa tgtgactgat ccgggacagc
     5521 agagctatat cacctggcgg gccgagtttc gtccccaggg cgggacccag tggcttgcgg
     5581 ccagccagca cacctttgcc gggacgatga ccccggggag ttccacgagc aaccagcggg
     5641 acagttattt tgccggtggg ctctacctga ttccgtacgg ccggaatgca acctacacca
     5701 tgagtaacgg gcccaatggc tacggctggc acgccgggaa aaatgcctac gattggccgc
     5761 ttcccatgaa caccatggtg ctctgtgccc gctcggggac cgttgtctcg ctcaaaaagg
     5821 attccaaccg cggtggtgcc tcgcagtcct accagaatga tgccaattac atccagatca
     5881 ggcatgatga tggcacgatc gccaactacc tgcacttcgc ctacaactcg attcttgtgc
     5941 gggtcgggca gagggttacg gtgggggaag agctgggccg gagcgggaat accggctgga
     6001 caacccagcc tcacctgcat ttccatgtct cgaacgcggc gggcaacgtg acctaccggc
     6061 tcaggttttt gacgcgcaat ggtccggtcg atccagcgtc agggacgcgg atgacgggtt
     6121 actatccctg gatgccgtaa gggtcttttt ttggggaaca gtctccagtc ccgcgatgcg
     6181 tgccgtatcg cgggatgaaa acatgttttt gcaatactgt gttgcgactc cggg
//
LOCUS       DAOSHE010000081         6098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11371_length_6098_cov_4.489326, whole genome shotgun sequence.
ACCESSION   DAOSHE010000081 DAOSHE010000000
VERSION     DAOSHE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_6098_cov_4.489326"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PLD82_03545"
     CDS             <1..1360
                     /locus_tag="PLD82_03545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOG64490.1"
                     /translation="HAAAVSLILEGAPRCTVLASDETIAFLSIRYGLPAEARTRKVRH
                     ASSERFGRFTLTALRVEHSIPGALAWLVEHERGSILYTGDFSGMLAETRDILGEKHIG
                     LAFCDCTNAPIPRPGPGEADVVAGIGDWIGRHADGRVLMTTFSSNLGRIKAALAAGRQ
                     AGMLLFVDGEAMLGAIGIIRSLDRDAVRGVAALEGSLPESPALILASGCQGEESSAFM
                     RLLSGASSLTDRDAIVISASPVPGNEEDWRAMLRLAASSRASLAVPPLEPVHASGHAV
                     RPVLGEIIGCIRPRILVPVHGDAFQRRALAGLGTECGIKTVLPGNGDQVRWDEVPGLV
                     FLGNSLQSGKHRRLPEVGAYERSLMARHGVACFVLRLNPPSGFAHVSLTMRGCLRADQ
                     HQKDLESLRDACEVEILDGYQSGTLGMSAIRRKIQAWAELELEERFGLRPLVIVSLVN
                     ES"
     gene            1544..2920
                     /locus_tag="PLD82_03550"
     CDS             1544..2920
                     /locus_tag="PLD82_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG64491.1"
                     /translation="MKPVVRNLLVGLRDFVIVLALLVWLARSDQMPQVVPPQVTDFLV
                     DMTDIVFGSGGGGAGAAATDLADLALYGETDREARRDLELYKARIENWFARVLDNVDG
                     IVTHPLVRRALPDFGQELSLMFAEDYLGGLITRYGDIDEILLHGQGGVFLRTTRENRE
                     PLPPEVVPQKILEKARAARGAILEPLPGQRFMLLASFLSPMRERQGTVVVVLNASTLK
                     KLVAEYPGSGDSRLYLHVRGNDPLIESGQGSWGALPERALRHERFLDTRAGRKRNLAL
                     PVRIAGSESGIIIGLLSPPVDSSKLLLLGVRFLILVLTIFVTLWLVRRILSGIWKIIR
                     THGMREQLLLHALDASLGHGKKILEGAKRITTVRPRVERRIIRTEAPRTESVRPKVAP
                     GAGMPADFQASGEPDLRRYERFHLDPRTPVAEGEFVIIDGPVDLPMDADGLTLPDMEP
                     AAPAPIDD"
     gene            2957..3355
                     /locus_tag="PLD82_03555"
     CDS             2957..3355
                     /locus_tag="PLD82_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOG64492.1"
                     /translation="MIMKVRGVFVCVWLFALISPAILGVGNIGTASPNPLLLDGTTTT
                     TSITVKTPSDLPSFDARVIIQDLSGRRVREYSRGSFGTSDYVVSWDGRNEAGRLVKSG
                     VYIVTVIRRYQDVSRGEETERFRLGLVRNQ"
     gene            3357..3725
                     /locus_tag="PLD82_03560"
     CDS             3357..3725
                     /locus_tag="PLD82_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOG64493.1"
                     /translation="MTSRILVLFFVVTLSIQGFALANETQAWAVPDPWMADAGLLRIN
                     VPSGHERVTIGIYRDDGAFVRELVPSVLEKGEELWQWDGKDDEGMRMPAGVYYIRVLS
                     VVNGYRISQILPLRILRRAR"
     gene            3722..>6098
                     /locus_tag="PLD82_03565"
     CDS             3722..>6098
                     /locus_tag="PLD82_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64494.1"
                     /translation="MNRKTLALSLLCTFLAAGLHGRPGGDTVPVIEMGVGGRALALGG
                     AYTALADDATAIVFNPAGLYAVDGDELTLSWNAMGGFFWNMGFAGYAHRFGEILTMGI
                     GTKFLLSGAQESWDSISEFNSFDENQYMLLLSASLDLPLALKLGASFKFAWRDFSWNS
                     SSGDQARSKTFANADLGVILPVARELQFGLRIENVIPSVDFGLGDAKEPVETRIVTGA
                     ALCLLDEKLVVSTDFDWYPGLGEVDFRLGLGYRVIAGWTLYTGLDSGRFDFGIGVDVS
                     DFSFHSSILLEAESEPSARFAVHYRVGKDKQEYRSIEQQLLALNKGIESFTKGDYAEA
                     RKQFRSVLQENPKNESARRLDQIAETRIRGEAWKTDDDRERIKSYVQKAENLAKNGSW
                     AEARKAATAALDIDPLEWKARDLVEKIDRSVDELVENHWRDGVAAFVRNDLEESRKKI
                     DALLSLRPEHREARDLLERIKRIQKDNWERKTNEERKLNAAELYRKRGMELFKRKIYV
                     DAIDSFKVSLEYGENASTRYYLNLAMQRYQDLKISGQSMSRSGEFLAIARDQIKNKKI
                     REAIASLEKALNYWPQNAEAERELETTRTALEAMVKGPYEEGLKAYKDGKYVEAIKKW
                     REAIAIDPDHEPSKKALADSAREMRERSLYNLQLAETWVRRGSDKDLLEALKYFRIAS
                     ELDPANEDAKKGFARTSARFEDLAKSLFDKGFDALKSGKPERLSEAIDSFERYLVIKP
                     DDKQARDFLDESVKKRSQQQSWLTMRQRRSEGIDFYNNREYERAAVKFREAL"
BASE COUNT         1230 a         1724 c         1874 g         1270 t
ORIGIN      
        1 ccatgccgcc gccgtcagcc tgatcctcga aggggctcct cgctgcacgg ttctggcgtc
       61 ggatgagacg atcgcctttc tctccatccg ctacggactt cccgcggagg cgcggaccag
      121 aaaggttcgg catgcgtcca gtgagcgctt tggccggttc accctgacag ccctccgggt
      181 ggaacactcc attcccgggg ccttggcctg gctcgtcgag catgagcggg ggagcattct
      241 ctatacgggc gatttttcgg gcatgctggc ggagacccgt gacatcctcg gagaaaaaca
      301 catcgggctg gcgttttgtg attgtaccaa tgcacccatc ccgcggcccg gtcccggcga
      361 agcggatgtt gttgcgggaa tcggcgactg gatcggacgg catgcggacg gacgtgtctt
      421 gatgacgacg ttcagcagta atctcgggag gatcaaggcc gctctcgcgg cgggacgcca
      481 agcggggatg ctgctctttg ttgacgggga ggcgatgctt ggcgcgatcg ggatcatccg
      541 cagtctggac cgggacgcgg tccggggcgt tgctgccctc gaagggagtc ttccggaatc
      601 tcctgccctg attttggcga gcgggtgtca gggcgaggag agctcggcct tcatgcggct
      661 tctttcgggt gcctccagct tgacagaccg ggatgccatc gtcatctccg ccagtccggt
      721 tccgggaaac gaggaggatt ggcgggccat gctccgcctg gcggcttcct cacgggccag
      781 cctggccgtt cctcctctcg aaccggtcca tgccagcggg cacgcggttc gacccgtcct
      841 tggggagatc atcgggtgca tccgcccaag gatccttgtt ccggtccacg gtgatgcctt
      901 ccagcgcagg gccttggccg ggttgggtac ggaatgcggg atcaagactg tccttccggg
      961 aaacggggat caggtccgct gggatgaagt tcccggtctc gtctttttgg ggaacagtct
     1021 ccagtccggc aaacacaggc gactccccga agtcggcgcg tatgagcgct ccctgatggc
     1081 ccggcacggg gttgcctgct ttgtgctcag gctcaatccc ccatccggat ttgcccatgt
     1141 ctccctgacc atgcggggct gtctgcgcgc cgaccagcac cagaaggatc tcgagagcct
     1201 gagggatgcc tgcgaggtcg agatattgga cgggtaccag tccgggaccc tgggaatgag
     1261 tgccattcgg cggaagatcc aggcatgggc cgaactggaa ctcgaagaac gcttcgggct
     1321 gcgccccctg gtgattgtca gccttgtgaa cgaatcctga tctggttcct ctccgttccc
     1381 ggcatcaggg cgatttgtcc gactgtatcg cctggtttcg ccagccgccg gatggaattc
     1441 ccgggatgac gagccactca gtatacccga agaatcctcc gatactgaag gagggttcct
     1501 gtccccgcgt ggctggaatc gttagaggag aggggagtct ggcatgaaac cggtggtgcg
     1561 aaaccttctg gtcgggttgc gggattttgt gattgtcctc gccctcctgg tctggctggc
     1621 gaggagcgac cagatgcccc aggttgttcc gccccaggtc acggattttc tggtcgacat
     1681 gacggacatc gtcttcgggt cgggcggagg tggtgccgga gcggcagcga ccgatctggc
     1741 ggatctggcc ctctatggag aaacggacag ggaagcccgc cgtgacctcg aactctacaa
     1801 ggcccggatt gaaaactggt ttgcccgggt gctggacaat gttgacggaa tcgtcacgca
     1861 cccccttgtc cggcgcgctt tgcccgattt tggccaggaa ctctccctga tgtttgccga
     1921 ggactatctg ggtggactca tcacccgcta tggcgacatt gacgagattc tccttcatgg
     1981 tcagggtggc gtcttcctgc ggacaacacg ggaaaaccgc gaacctcttc cgcccgaggt
     2041 tgttcctcaa aaaattcttg aaaaagcacg agccgctcga ggcgccatcc tcgagcccct
     2101 cccgggccag cgcttcatgc tcctggcctc ctttctgtcg ccgatgcggg agcgtcaggg
     2161 gactgtcgtt gttgtcctga atgcttcgac tctgaaaaaa ttggttgccg aatatcccgg
     2221 atccggagac tcgaggctct acctccatgt ccgcgggaat gatcccctta tcgagtccgg
     2281 tcagggttca tggggggctc tcccggaacg ggctttgcgg cacgaacgtt ttcttgatac
     2341 ccgtgccggc cggaagcgga atctggccct ccctgtcagg atagccggtt cagaaagcgg
     2401 gatcatcatc gggcttcttt ctccacccgt cgattcatcc aaactcctgc ttctgggagt
     2461 ccgcttcctg attctcgtcc tgacgatttt tgtgacactc tggctggtcc gccggattct
     2521 ctcagggatc tggaaaatca tcaggacaca cgggatgcgc gaacaattgc tgctccatgc
     2581 ccttgatgca tcccttggcc acgggaaaaa aattctcgaa ggggccaagc ggatcacaac
     2641 cgtcaggcca agggtggagc ggcgaatcat ccggaccgaa gctccacgga ccgaaagtgt
     2701 ccggcccaag gtcgctccgg gtgccggaat gcccgccgat tttcaggcca gcggtgagcc
     2761 ggatctgcgc aggtatgaac ggtttcatct cgatccccgg actccggttg ctgaagggga
     2821 atttgtcatc attgacggcc ctgtggattt gcccatggat gctgatggct tgacacttcc
     2881 cgatatggaa cccgcagcac cggcaccgat tgacgattga tcccccggga tgggatatac
     2941 tcgagagtgg aggacgatga tcatgaaagt acggggtgtg tttgtttgcg tctggctttt
     3001 cgcgctcatt tctccggcca ttctcggtgt cgggaatatc gggacggcca gtcccaatcc
     3061 tctcctcctt gacggaacaa ccacgacaac cagtattacc gtcaagacgc cctcggattt
     3121 gccttccttc gatgcccgtg tgatcattca agacttgagc ggccgcaggg tcagggagta
     3181 ttcccgcggg agtttcggga cgagcgacta tgtcgtttcc tgggatggcc ggaacgaagc
     3241 gggccgcctg gtcaagagcg gagtctacat cgtcacagtc atcagacgct atcaggatgt
     3301 ctcccggggc gaagagacgg aacgcttccg tcttggcctg gtcaggaacc aataatatga
     3361 cgagccggat tcttgtactc ttctttgttg tcacactctc cattcagggc tttgccctgg
     3421 ccaacgagac acaggcctgg gccgtcccgg atccctggat ggctgacgcc gggctcctgc
     3481 ggatcaatgt tccgtccggt catgaacgtg tcaccatcgg gatttaccgg gatgacggcg
     3541 cttttgtccg ggagcttgtt ccttccgtgc tggaaaaagg ggaagaactc tggcagtggg
     3601 acggaaagga tgacgagggc atgcggatgc cggccggggt ctattacatc cgggttctct
     3661 cggttgtgaa cggataccgg ataagccaga ttctgcccct gcgaatcctg cggagggccc
     3721 gatgaaccgg aagaccctgg cactctctct cctctgtacc ttcctggccg ccggtttgca
     3781 tggccggccg ggaggggata ccgttcccgt catcgagatg ggtgtgggcg gaagagccct
     3841 cgcccttggt ggggcctata ccgctctggc cgatgatgcg accgccattg tcttcaatcc
     3901 ggcggggctg tacgctgtgg acggtgacga gctgaccctc tcctggaatg cgatgggcgg
     3961 gttcttctgg aatatggggt tcgccggcta tgcccaccgg tttggagaga tcctgaccat
     4021 gggaatcggg accaaatttc tcctctccgg tgcccaggag agctgggatt cgatcagcga
     4081 gttcaacagc tttgacgaga accagtacat gcttctcctc tcggcctccc tcgatctgcc
     4141 cctggccctg aaactcggcg ccagtttcaa atttgcctgg agggattttt cgtggaactc
     4201 cagttcgggt gaccaggcac gttcaaaaac cttcgccaat gccgatctgg gagtcatcct
     4261 tccggtggct cgcgagttac aattcggact ccggattgaa aacgtcattc cctcggtgga
     4321 cttcggactt ggtgacgcga aagaaccggt cgagacccgg atcgtgaccg gagcggccct
     4381 gtgccttctg gacgagaagc tcgttgtcag taccgatttt gactggtatc cggggttggg
     4441 cgaggttgat ttcaggctcg gtctgggata cagggtcatt gccggctgga ctctctatac
     4501 cgggcttgac agcggacggt ttgatttcgg gatcggtgtc gatgtcagtg atttttcatt
     4561 tcattcaagc atcctcctcg aggccgagag tgaaccatcg gcacggtttg ccgtccacta
     4621 ccgggtcgga aaggacaagc aggagtatcg ctcgatcgaa cagcagctgc tggccctcaa
     4681 caaggggatc gagagtttca ccaaaggcga ctatgccgaa gcccgcaaac agttcagaag
     4741 tgtccttcag gaaaatccga aaaacgagag tgctcggaga ctcgaccaga tcgccgagac
     4801 ccggatccgg ggcgaagcgt ggaagacgga cgatgaccgc gaacggataa aaagttacgt
     4861 ccaaaaagcc gagaatcttg ccaagaacgg atcctgggca gaggcacgca aggctgcgac
     4921 cgccgccctg gacattgatc cgcttgaatg gaaggcccgg gatctcgtcg aaaaaataga
     4981 ccgcagcgtc gatgaattgg tggaaaacca ttggcgggac ggggtcgccg cctttgtccg
     5041 gaatgacctc gaggaatccc ggaaaaaaat tgatgcactc ctgtctctca gaccggaaca
     5101 ccgcgaggcc cgggatcttc tggaacgcat caaacggatc cagaaggaca actgggagcg
     5161 gaagaccaac gaagaacgca agctcaatgc ggcggagctc taccggaaac gggggatgga
     5221 actcttcaag cgcaagatct atgtcgatgc cattgacagc ttcaaggtct cgctcgaata
     5281 cggggagaat gccagcaccc gctattacct caatctggcg atgcagcgct atcaggatct
     5341 caagatcagc ggacagagca tgagccggtc cggggaattt ttggcgattg cgcgcgacca
     5401 gatcaaaaac aaaaaaatcc gcgaggcgat tgcttctctc gagaaagcgc tcaattactg
     5461 gccgcaaaac gccgaggccg agcgcgagct tgagacgacc cggaccgcac tggaagccat
     5521 ggtcaagggc ccctacgagg aaggactgaa agcctacaag gacgggaagt atgtcgaggc
     5581 gatcaagaaa tggcgcgagg ccatcgccat cgatccggac catgaaccgt caaaaaaagc
     5641 attggcggat tcggcccgcg agatgcggga gcgttcgctc tacaatctcc agcttgccga
     5701 aacctgggtc aggcgcggga gtgacaagga tcttttggag gccctcaagt atttccggat
     5761 tgcgagcgaa cttgatccgg ccaacgagga tgcgaaaaaa gggtttgcgc gcaccagtgc
     5821 ccgcttcgaa gatctggcaa aatccctctt tgacaaagga ttcgatgccc tcaagagcgg
     5881 aaaaccggag cgtttgagtg aagcaatcga ctcgtttgaa cgctatcttg tgatcaagcc
     5941 cgatgacaag caggccaggg attttcttga cgagtcagtc aaaaaacggt cccagcagca
     6001 gtcctggctc acgatgcgcc agcgaagaag cgaggggatc gatttttaca acaacaggga
     6061 atacgagcgg gccgcggtca aattccgcga agccctgg
//
LOCUS       DAOSHE010000082         6065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11458_length_6065_cov_3.724126, whole genome shotgun sequence.
ACCESSION   DAOSHE010000082 DAOSHE010000000
VERSION     DAOSHE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_6065_cov_3.724126"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1692)
                     /locus_tag="PLD82_03570"
     CDS             complement(250..1692)
                     /locus_tag="PLD82_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64495.1"
                     /translation="MISQELIIIIGGAVAAAFAVIGILARRGVAKNLDDYIIFRNRTG
                     SLAATCSVVACLTGAWILYSVPEAAAGYGVAAIVGYAIGQGLPMLLLVPVGARVRREY
                     PQGRGLSDYVEARFGRSAALFVLGFMFLYMLVFLCAEMTAVGGAFNTVAGFPVWATAA
                     AVMAFTLAYAVYGGLPSSILTDVVQFLVILPLLLLVLVAVFGGSVDRVDLTSKQALRL
                     FDFGSWSGIQFGIVLIIGVTASNLFHQGFWQRIYSCTTEKVMKRSFGIAGVITIVMIA
                     LAGVFGLLAGGAGMVGGKGQPAAAQSLFVLIKFISPVFLIVVLVLALSLTMSSSDTLL
                     NAMASILMLRKAGQPHDEKKALKLARSITALLGVLAVLVAALNISVLYLFLVVDLLAA
                     ATIVPVIAGLWWKRYGLMTLVASSLSGLVVGGLFFPGPDYVSLITASWWPGATLSSDT
                     LFLLSFGLALTVSTVVTLAGEKGFKKKIAG"
     regulatory      complement(1804..1917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2161..2811)
                     /locus_tag="PLD82_03575"
     CDS             complement(2161..2811)
                     /locus_tag="PLD82_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64496.1"
                     /translation="MKQSVLLAGLVLAFAMLVACGKSGDAPAKGGDEGAKKQETVVSA
                     KKRYAVKSGKIESEFGPKGMKLKRVYWFDDFGTKERVETSLGDKLTEINIYDGSHKYT
                     IRMSNDEKVAWKEAWRYGLMKDVNIDQVKNGKGVTILPSMTILGFTCDAYKAEYQGMV
                     VTMAGYKGVQLLMESTGKMAVTDKATKAEFDIDVPAERFAVPDGYTVKDPAEAGKK"
     gene            3272..4549
                     /locus_tag="PLD82_03580"
     CDS             3272..4549
                     /locus_tag="PLD82_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG64497.1"
                     /translation="MLDSPVFLALVWGIVSSLSMPGGALLGITLTPPARFLSILMAFG
                     AGALLFAVSFEIFAGAAEKAPLMLGLGAISGGILFGVINRQLNDSGAFIRRTSLIRRQ
                     IVREKRRFARQAIAHLSRVTLFRSLPIPLVVRLAPNIRVEDVPEGTLFFREGDQGDRL
                     YCIVAGEVRISRGRRTIATLGDNETFGEMALVTRNPRNATARAMVDSRIFSIPHDDFQ
                     AVLSESPELRSDVESLVSKRFDELVASGTATRAQDWKRSGLSYLRDMAIQVTANEIAS
                     LPANLIKRKNVALAIWAGILMDSIPASLVIGMSSTSTAGISLALIAGVFLADLPESIT
                     CAAHMQRGGMSRKRILLLWTSISLLTGLGAMVGALLPGAETAGGMRFLEGMAGGAMLA
                     SIAEAMLPDAAEGGSGITGLATVTGFMAALALR"
     gene            4759..5985
                     /locus_tag="PLD82_03585"
     CDS             4759..5985
                     /locus_tag="PLD82_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HOG64498.1"
                     /translation="MFLEKSFRHCDYYDLLSPENLYKILVKMAPWLPREGDPVFLVLV
                     FLILTSDNPFTRAASEGRTINPLTLAIAKRELESLEVIARTGAKRVFDIFSMTKACQP
                     LEFDGISVCDEPCERTPLPEFQRMLDLFASPVAWGNHIPELERWHRKWGYGVGIHHLA
                     FNFENDSIYGFDFTPLFNTAIPHPSDFIGYEDERTTIRENTRRFVEGLPAHDMLLYGD
                     RGTGKSSTIKLMLREFSGQGLRMIATDNNVKLLTELFSTVRNHRWKYILFIDDISFES
                     WQSSSALEFRSLIQGDLIDRPKNVIFYVTSNRRTIVRMETDDTESHSSKLSAEDRQEI
                     LALSDRFGVKIWFQTPTQEQYLDIVRGLARQYGIAMAEDELVGKALQWERWNNVRSPR
                     TAHQFIRSLVTTPLPD"
BASE COUNT         1396 a         1861 c         1555 g         1253 t
ORIGIN      
        1 aattcaaggg tcagaaaaac ggcttgggag ttgtcgtcaa aaaggcgacg gcacggacag
       61 gcggcggcgg agatttccgc ggtggagatt cacgtggtgg caaaccgccc tacaagggcg
      121 gtccccgccg gcgctaacca cagcggaggc ttgcacagca aagcctcgcc ggaccagctt
      181 tgtcacaacc catgaaaatt caggacgcca tcacccagaa aaaaataatg gggatggcgt
      241 ccttctgtcc tatcctgcga tctttttttt gaaacccttt tcaccggcca gcgtcacgac
      301 cgtcgagact gtcaaggcca gtccgaagga cagcaaaaag agcgtgtcac ttgagagtgt
      361 tgcaccgggc caccaagaag ccgtgatcaa gctgacatag tccggacccg gaaaaaagag
      421 tccaccgacg accaggcccg aaagactgga tgcgaccagt gtcatgagtc cgtagcgctt
      481 ccaccagagt ccggcaatga cgggaacgat ggttgcagcg gccagaaggt caaccacaag
      541 aaaaagatag agcacggaga tgttgagggc tgcgaccaaa accgcaagga ctccgagaag
      601 cgccgtaatg cttcgcgcga gtttaagcgc ctttttctcg tcatgcggtt ggccggcctt
      661 gcgcagcatg aggatgctgg ccatggcatt taacagggta tcgctcgaac tcatcgtaag
      721 cgagagggcc aggaccagca cgacaatcag gaaaaccgga gagatgaatt tgatgagaac
      781 aaagagtgac tgggctgcag cgggttgacc cttgccaccc accatcccgg caccaccggc
      841 caagaggcca aagacaccgg ccagggcgat catcacgatc gtgatcacgc cggcaatccc
      901 gaaggagcgc ttcatcactt tttctgtcgt gcacgaatag attctctgcc aaaatccttg
      961 atggaaaagg tttgaagccg ttacaccgat gatgaggacg attccgaact ggatcccgga
     1021 ccaggaacca aaatcaaaga gccggagagc ctgtttggaa gtcagatcga ccctatcgac
     1081 actcccaccg aaaaccgcca caaggacaag caacaagagc ggcaggatca ccaaaaactg
     1141 gacaacgtcc gtcaggatgc tggagggcag gccaccgtag actgcatagg ccagggtaaa
     1201 agccatcacg gccgccgccg ttgcccagac gggaaaaccg gccacggtat tgaatgcgcc
     1261 accaaccgcc gtcatctcgg cacacaaaaa gaccagcata tagagaaaca taaatccgag
     1321 aacaaaaagc gctgcactgc gcccgaagcg ggcctcgaca taatccgaaa gcccgcggcc
     1381 ctgcggatac tccctgcgca cgcgcgcccc gaccgggacc aggaggagca tcggcaagcc
     1441 ctgtccgatg gcatatccga caatcgccgc cacaccatag cctgcggctg cttcgggaac
     1501 cgagtagaga atccaggccc cggtcagaca ggccacgacg ctgcaggtcg ccgccagcga
     1561 gcctgtccgg ttgcgaaaga tgatgtagtc atcgagattt tttgcgaccc cgcgacgggc
     1621 cagaataccg atcacggcaa aggccgccgc gacggcaccg ccaatgatga tgatcaattc
     1681 ctgagaaatc atggtaccac ctccgacttt tgttgatgaa caaccccgga cgagcggggc
     1741 tcgacttgcc ctgccggtat tggctgttca ctgtcacgtc tgccggagat gcccacggga
     1801 tatcgcggga aatgatcgcc ttcctccgcc ggtatgatcc ggatcaggtt acaagggttt
     1861 ggcgcgttca accgcgccct ctcagccaca ctccatgcgg cacccctggc gttcgcaacc
     1921 gcttaatagt agccagagaa tcaggatccg tatagaagaa tgtgcgaaaa ctgtccggcg
     1981 atgacaccct gacactgcat gattttgcga tacaagaatc ggactccggg acacacatga
     2041 aaacatcatg attttctgct gtgactccgg gaaacacaac acggttttgt aatggacgca
     2101 agaccagccg cgaaaaaaac cagcccttgc gaggctggcc ttggagttac aaacctgatt
     2161 tcattttttg ccggcttcag cgggatcctt gacggtatac ccgtccggta ccgcaaatcg
     2221 ttcggccgga acatcgatat cgaactcggc cttggtggcc ttgtcggtga cagccatctt
     2281 gccggtggat tccatcaaaa gctgcacacc tttatacccg gccatggtga cgaccatacc
     2341 ttggtactcg gccttgtacg cgtcacaggt aaacccgagg atggtcattg agggcagaat
     2401 agtgaccccc ttgccatttt tcacctgatc gatattgacg tccttcatca atccgtagcg
     2461 ccaggcttcc ttccaggcga ccttttcgtc attgctcatc cggatcgtat acttgtggga
     2521 tccatcgtag atgttgattt cggtcagctt gtcaccgagt gaggtttcaa ccctctcttt
     2581 ggtgccgaag tcgtcaaacc agtagactcg cttgagcttc atacctttcg gaccgaattc
     2641 ggactcgatt ttgcccgact tgaccgcata gcgctttttg gcagaaacaa cggtctcctg
     2701 ctttttcgct ccttcatcac cacccttggc aggcgcatcc ccgctcttgc cacaagcgac
     2761 aagcatggca aacgcgagca ccagtccggc cagcaacaca ctctgcttca tacatcctcc
     2821 gtatacaatg atttcccgct tcagcgggtc ggtcattgaa tgaacattca ctcaacagag
     2881 gacatcgtat cacatggaca cgatctgtca atttgtttgg tccgaaaaaa acaaaaaatc
     2941 cgtatttcta cgaattgtca cggagaccac attcaacgat acttgtccta tgcaagctcc
     3001 cttgggagcg gatcctgcag cactgttgaa caccttgcat aagaattatc aacccgcacc
     3061 ggggccgtgt ctgcagggac acggcccttt ttttgctctc tctgcctccc tctacgggcg
     3121 aagtgctcgt caagcgtacc cttcagccgg ctccctgagt gcgaaatcaa cacatgcgag
     3181 tccttcgctg gcagtggaaa ctttggacgg atttgacccg gctcgcgctt cgtgctatac
     3241 tgcacccata ccggccacaa ggagttcatc catgcttgac agcccggtct ttctggcctt
     3301 ggtctggggc attgtctcgt cgctctcaat gcccggcggc gctctcctgg ggataaccct
     3361 cactccgccg gcccggtttc tctccatcct gatggccttc ggtgccggcg cccttctttt
     3421 tgccgtttcc ttcgagattt ttgccggcgc cgccgaaaaa gcccccctga tgcttggact
     3481 cggtgcgatc tcgggtggca tcctctttgg tgtcatcaac cggcaactca acgacagcgg
     3541 cgccttcatc cgccggacca gcctgatcag gcgccagatt gtgcgtgaaa agcgacgctt
     3601 cgcccggcag gcaatagccc atctctcgcg ggttacgctc ttccgcagcc ttcccatccc
     3661 tcttgttgtt cgcctggctc ccaatatccg ggtcgaagat gtccccgagg gaacgctctt
     3721 ttttcgcgag ggcgatcagg gtgaccgtct ctactgcatc gtcgccggag aagtccgaat
     3781 cagccgtggc cgccgtacca tcgccaccct cggagacaac gaaacattcg gcgaaatggc
     3841 cctggtcacc cgcaatcccc gcaacgccac agcccgcgcc atggtggatt cgcggatctt
     3901 ctcgatcccg catgatgatt ttcaggccgt cctctcggag tcacccgagc tgcgatcgga
     3961 tgtcgaatct ctggtctcaa aacgcttcga tgaacttgtg gcaagtggta cggccacccg
     4021 ggcccaggac tggaaacgca gcgggctctc ctacctgaga gatatggcca ttcaggtgac
     4081 tgccaacgaa atcgccagcc ttcccgcaaa cctcatcaaa cgaaagaacg tagccctggc
     4141 catctgggcc ggaatcctga tggactccat ccccgcatcc ctggtcatcg ggatgtcatc
     4201 gacctcgact gccggaatca gtctggccct gatcgccggt gtctttctgg cggatctccc
     4261 cgaatccatc acctgcgccg cccacatgca gcgcggcggg atgtcccgca aacgcattct
     4321 ccttctctgg acatccatct ccctcctgac cgggctcggt gccatggtgg gtgcgcttct
     4381 tcccggagcc gagactgccg gcggaatgcg ctttcttgag ggaatggccg gcggggccat
     4441 gctggcatca atcgccgagg ccatgctccc cgatgccgcc gaaggcggaa gcgggattac
     4501 cgggctggcc accgtcaccg gtttcatggc cgccttggcc ctgcgctgaa aaaatcctgt
     4561 ttaacaaatt tccgcaatac acgacagggt atgtgcgtat tcctgatcat acacagcaac
     4621 aaacggaggc agcccatgga caaagccgag tacaaactct tgaacaaaaa cacactctcc
     4681 aaacgtaccc tcaacagaca ggttggcatc gtatcccatg ccggacatat gccatactac
     4741 aatttgagga atatatatat gttcctggaa aaaagtttcc gtcattgtga ctattatgat
     4801 ttgctcagcc cggaaaacct gtacaaaata ctcgtcaaaa tggcaccctg gcttccccgg
     4861 gaaggagatc ctgtcttcct tgttctggtc tttctcattc tgacctcgga caatccattt
     4921 accagggccg cgtccgaggg gcgcaccatc aatcctctga ccctggccat cgccaaacgg
     4981 gaacttgaat ctcttgaagt gatcgcacgg acaggcgcga aacgtgtttt tgatatcttc
     5041 tcgatgacaa aagcctgcca gccgctcgag tttgacggaa tttctgtctg tgacgaaccc
     5101 tgcgagcgta ccccgctccc cgaattccag cgcatgcttg atctctttgc ctccccggtt
     5161 gcctggggca accacattcc cgagcttgaa cgctggcatc gcaagtgggg ctacggagtg
     5221 gggatccacc atctggcctt caacttcgag aacgacagta tttatggctt cgacttcacc
     5281 cctctcttca ataccgccat tccccacccg tccgatttca tcggatacga ggatgagcgg
     5341 acaaccatca gggagaacac gcggcgtttt gtggaaggcc ttccagccca cgacatgctt
     5401 ctctacggcg accgtggaac cggcaaatcc tcgaccatca aactcatgct gcgggaattc
     5461 tccgggcaag gattgcgcat gatcgcaacc gacaacaacg tcaaactcct cacagaactt
     5521 ttttcaacag tccgcaacca tcgctggaaa tacatcctct ttatcgatga catctccttt
     5581 gagtcctggc aatccagcag cgccctcgag ttccgctccc tgatccaggg agacctgatc
     5641 gatcggccca aaaacgtgat cttttacgtg accagcaacc gccgcacaat cgtccgcatg
     5701 gaaaccgatg acaccgaatc gcattcttca aagctttcgg ccgaggaccg ccaagagatc
     5761 ctggctctct cggaccgctt cggcgtcaag atctggttcc agaccccgac ccaggagcaa
     5821 tacctggata tcgtccgcgg actggcccgg cagtacggga tcgcgatggc cgaggatgaa
     5881 cttgtgggca aggccctgca atgggaacgc tggaacaacg tacgctcccc tcgcaccgcc
     5941 catcagttca tccgcagtct ggtcaccaca ccactcccgg attgacactg aggtatcaat
     6001 cagccttgcg gatcatgatc aggagcatct tgaacttccg gagggccttg agggcgtgcg
     6061 ggatt
//
LOCUS       DAOSHE010000083         6040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11531_length_6040_cov_3.688889, whole genome shotgun sequence.
ACCESSION   DAOSHE010000083 DAOSHE010000000
VERSION     DAOSHE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11531_length_6040_cov_3.688889"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1136)
                     /locus_tag="PLD82_03590"
     CDS             complement(168..1136)
                     /locus_tag="PLD82_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOG64499.1"
                     /translation="MLLNILLLLAGIAGLVVSADILVRGASSIARRLSVSDLVVGLTV
                     VAFGTSAPELVVNIVASSEGNGQVVFGNIIGSNIFNILGILGVAGLIRVLAVSKSTAW
                     REIPFVVAGGILLLFLVLDGWHGGENQLSRLDGVVLLVAFTGFFAYVFRMALRGRAET
                     NAGTGYIGEMPLVLAVLAVLGGLIGLFFGGRLVVEQATALARSLGVSDRLIALTIVSA
                     GTSFPELATSAVAAWRGKADIAIGNVVGSNIFNVLFILGVSALIKPVTWNGEADMTID
                     LGIMIASSLLLFGAMYTFGRRKLDRIEAGIFLVGMLAYTLWVIERG"
     gene            complement(1149..1700)
                     /locus_tag="PLD82_03595"
     CDS             complement(1149..1700)
                     /locus_tag="PLD82_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64500.1"
                     /translation="MMLKRTGIVVLAMVLFVCAGIVSAAPLATLTVRYNGTLEKVDLF
                     VNAVKRGEIAQGKTVSIQLPANVSYTVTLRSGDLVETKTAWLASGLVRQLVFFGPNAP
                     AEGESSTGVILLQLAGRYDRADVFVNDGAMGSVQRGHMREIKVKSGETYKVTVRRDGR
                     EASLTVYVGVGGSLSRKTVQLSL"
     gene            complement(1902..2375)
                     /gene="ilvN"
                     /locus_tag="PLD82_03600"
     CDS             complement(1902..2375)
                     /gene="ilvN"
                     /locus_tag="PLD82_03600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOG64501.1"
                     /translation="MVHTISALVENHFGVLTRVAGLFASRGYNIQSLTVGPTEDETVS
                     RMTIVVEGDDKIIDQVKKQLNKLIDTIKVIDLTGEDFVDRELLLIKVTASSKTRPDIL
                     QLTEVFKGSVVDIAPAVVTIEVSGNPKKIDAFIAMLKPYGVREMARTGRIALGRG"
     gene            complement(2375..4099)
                     /gene="ilvB"
                     /locus_tag="PLD82_03605"
     CDS             complement(2375..4099)
                     /gene="ilvB"
                     /locus_tag="PLD82_03605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOG64502.1"
                     /translation="MKGAQILVECLKREKVTTIFGYPGGQLIPIFDALYDAREINLVL
                     TRHEQGAAHAADGFARASGRVGVCMATSGPGATNLVTGIATAYMDSVPMVCITGQVPT
                     SLIGNDAFQEADIVGITRPITKHNYLVKDVCDLARTVREAFFIASSGRPGPVLVDIPK
                     DIAVADTEFIYPETVSIRGYNPTLEGNPRQIKRAAELIMASKRPIIYAGGGVIISGAH
                     EELLQFAEKTGIPVTMTLLGLGAFPNAHPLSIDMLGMHGTVYANNAVCDSDLIISVGA
                     RFDDRVTGKLDEFAPHAKIIHIDIDPTSVSKSVEVDVPIVGDAKHVLQELLPQVSKLS
                     IDDWLTRIRDWKTRYPLAYGEEAGIIKPQAVIRKLSELTKGEAIITTEVGQNQMWAAQ
                     FYNFTSPRSFITSGGLGTMGYGLPAAIGAQFAFPDKTVIDIAGDGSIQMNIQELATAV
                     VNNLPVKVLILNNTYLGMVRQWQELFYDKRYAATCLDRREGCPDGCSTPGDHCPSYVP
                     DFVKLAEAYGAYGRRVTNPAEVESALVDMLNHKGTAILEVRIAKEENVYPMVPAGAAL
                     TQMIGGMA"
     gene            4390..5574
                     /locus_tag="PLD82_03610"
     CDS             4390..5574
                     /locus_tag="PLD82_03610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG64503.1"
                     /translation="MAVSRKPPAAKKKPAPLPVREEEIGDLETSLQEWDTGLFDNYEK
                     KILDLKNLVQISGLINTEMNMSEMTQSLLYSCQGMMLVRSVTLFILKSIVSNVYAYGA
                     SVGFANESFAEEFSDEDPLVTALLERKKTLWIREKAADSSIQTSLLRLAALEPDLIIP
                     MLYKDKLTGFLVVGQKLTGETFTSSEVEFMANMANLAAIAIENLRLFEMATRDRMTNL
                     YVHHYFQNRLEEEIARAIRYHNHRLALLMFDIDHFKSVNDTWGHQAGDVIIKEIAGII
                     KENLRKLDLPARYGGEEFAVILPESDVNNARLIAERIRQAVESRVFDIGNGKQIQVTI
                     SAGASDLSMAVAASEQMEPAQMSRESQMREVLIGQADTALYCSKHGGRNKATVFTPGM
                     TH"
     gene            complement(5629..>6040)
                     /locus_tag="PLD82_03615"
     CDS             complement(5629..>6040)
                     /locus_tag="PLD82_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017713829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOG64504.1"
                     /translation="FVGVSAPGIVSREMVQSMQPKPIVFGLSNPVPEVMPEVLDGLDA
                     VIATGRSDYPNQINNVLAFPGIFRGALSCRSRSITPAMYRAAAGALAGLVSVRDREAG
                     RIIPAVFDPRVAGAVSAAVVECAVAEGLARRMPE"
BASE COUNT         1419 a         1840 c         1547 g         1234 t
ORIGIN      
        1 tacgatccag tcgaaaccgg tacaccccct cacaattctt gaagccagac tgatacaagc
       61 ttccttctgt atctcaagta tacgacaaaa gaggccccgg ttttgctcaa aattgcatga
      121 aaacggaatt tcttccggtt gcaaactcga cctgaagaat ccgggagcta ccctctctca
      181 atgacccaga gcgtgtaggc cagcatcccc accaaaaaaa ttccggcttc aatccggtcg
      241 agcttgcgcc ttccgaatgt atacatcgcc ccgaataaaa gaagggatga cgcgatcatg
      301 attccgagat caatggtcat atccgcttcg ccattccagg taaccggctt gatcagggcc
      361 gataccccca atatgaagag aacattgaag atgttgcttc caacaacgtt cccgatggca
      421 atatcggcct tcccgcgcca ggctgcaact gcagaagtcg ccagttcagg aaaagacgtc
      481 ccggccgaca caatggtcag tgcaatcaat ctgtcactga cacccagact cctggccaat
      541 gccgtcgcct gctcgaccac aagccttcca ccaaaaaaca ggccaatcag accaccaagg
      601 acagcgagca cagccagcac aagcggcatc tcaccgatat atcccgtgcc ggcattcgtt
      661 tccgcccttc ctcgaagagc catcctgaag acgtaggcaa aaaatccggt gaacgcgaca
      721 agcaggacaa caccatccag ccggctcagc tggttttcac caccatgcca cccatccaaa
      781 acgaggaaca agagcaggat tccgccggca acaacaaagg gaatctcacg ccaagccgta
      841 ctcttcgaga cagcaagaac ccggatgagc ccggcaactc cgagaatccc gaggatattg
      901 aagatattgc tgccgatgat attcccgaac accacctgtc cgtttccctc ggagctggca
      961 acaatgttga cgacaagttc gggggccgag gttccaaaag caaccactgt caatccgacg
     1021 actaaatcgg aaaccgagag gcgtctcgct atggaagacg cgcccctgac cagaatatcc
     1081 gcagagacaa cgagcccggc gatcccggca agaagcagga ggatgttcaa caacattctg
     1141 cagtcccctt acagactgag ttgtacggtt ttccgggaaa gactcccgcc gacaccaacg
     1201 taaaccgtca gactggcttc ccgcccgtca cggcgcacag tcaccttgta tgtctcaccg
     1261 gatttgacct tgatttcccg catatgcccc ctctggacac tccccatcgc gccatcattg
     1321 acaaagacat ccgcccggtc atatctgcca gcaagttgca aaagaatgac gcccgtgctg
     1381 ctctcacctt ccgccggagc attcgggcca aaaaaaacaa gctgcctgac caaaccactc
     1441 gccagccagg ccgtctttgt ctcaaccagg tcgccgctcc ttagggtgac ggtatacgaa
     1501 acgtttgccg gcaactggat ggacactgtc ttcccctggg cgatctcccc ccgcttgacc
     1561 gcgttgacaa ataaatccac cttttcaagg gttccattgt atcgcactgt gagggttgcc
     1621 aacggcgctg ccgagacaat cccggcgcaa acaaacagca ccatagcgag aaccacaata
     1681 ccagttcgct tcagcatcat gacaccaccc tccatggaat gaaaaagact ttcactagat
     1741 tatacaccga acagccccgg gccgtcatcc gattcaccgc cactacgtga cggaaagccg
     1801 cgtcacgtgg accagatcgg gatcaagacc gcgctgaaga gggccagaga tgaaaaaagc
     1861 cccttcccga ggactgatgc ccgcgagaag ggggatgact gtcaccctct cccgagagca
     1921 atccgtccgg ttcgtgccat ctcccggaca ccatagggct tgagcatggc gatgaaagca
     1981 tcgatttttt tgggatttcc gctgacttcg atcgtcacaa ccgccggggc aatgtcaacg
     2041 acgctcccct tgaagacctc ggtcaactgc aggatatccg gccgggtctt gctggacgcg
     2101 gtgaccttga tcaggagcaa ttcccggtcc acaaagtctt ctccagtcag atcgatcacc
     2161 ttgatcgtat caatcagctt gttgagctgc tttttgacct gatcgatgat cttgtcatct
     2221 ccctcgacga caatggtcat ccttgagact gtctcgtctt ccgttggccc gactgtgaga
     2281 ctctggatgt tgtatccccg gcttgcaaaa agcccggcaa cacgggtcag gacaccgaaa
     2341 tggttttcga caagtgctga aattgtatgc accatcaggc catccctcca atcatctggg
     2401 tcagggcagc accggcggga accatcggat atacgttttc ttctttggcg atccgcactt
     2461 caaggatggc tgtccccttg tggttgagca tatcgacaag agccgactca acctcggccg
     2521 gattggtgac ccggcgaccg tacgcaccat aggcctccgc cagcttgaca aaatccggca
     2581 cataggacgg acaatgatca cccggagtgg agcatccatc cggacatccc tcccgcctgt
     2641 ccaggcaggt cgctgcgtag cgcttgtcat agaacagctc ctgccactgc cggaccattc
     2701 cgagataggt gttgttgagt atcagcacct tgacgggaag attgttgacc acagcggtcg
     2761 ccagttcctg gatgttcatc tggatcgacc cgtctccggc tatgtcgatc actgtcttgt
     2821 cggggaaggc aaactgcgcg ccgatcgccg caggcaatcc gtatcccatg gtaccgagtc
     2881 cacccgaggt gataaaactg cgcggactgg tgaaattata gaactgggca gcccacatct
     2941 ggttctgtcc gacctcggtg gtgatgatcg cctccccctt tgtcaactcg gagagtttgc
     3001 ggatcacagc ctgcggtttg atgattccgg cttcttcgcc ataggccagc gggtaacgtg
     3061 ttttccagtc cctgatccgg gtcaaccagt catcaatgga gagtttcgaa acctgtggca
     3121 agagttcctg caacacatgt tttgcgtcgc ccacgatcgg tacgtcaacc tcaacgctct
     3181 tcgaaaccga ggttgggtcg atatcgatat ggatgatctt tgcatgggga gcaaattcgt
     3241 caagcttgcc ggtcacccgg tcatcaaacc gtgccccgac cgagatgatc agatcgctgt
     3301 cgcacacggc gttgtttgca tagacggtcc cgtgcatccc gagcatgtcg atcgaaagcg
     3361 gatgggcatt gggaaaagcc ccgagaccca gcagggtcat ggtgacggga atacctgtct
     3421 tctcggcaaa ctggaggagt tcttcatgcg cacccgagat gatcacgccc ccgccggcat
     3481 agatgatcgg ccttttggaa gccatgatca gttcggcggc ccgctttatc tgacgcggat
     3541 ttccttcgag cgtcggattg tacccccgga tcgagaccgt ttcggggtag ataaattcgg
     3601 tatcggcaac ggcaatatcc ttggggatgt ccaccaagac aggacccggg cggccactgg
     3661 atgctataaa aaatgcttcg cggacagtcc tggcgagatc acatacgtcc tttaccaaat
     3721 agttgtgctt tgtgatcggt ctggtgatcc cgacaatgtc ggcttcctgg aacgcatcgt
     3781 taccaatgag gcttgtggga acctgtccgg taatgcagac catgggcaca ctgtccatat
     3841 aggccgtggc gatgcctgtt accagattgg ttgcaccggg tccggaggtt gccatgcata
     3901 ccccgacccg tccgctcgcc cttgcaaaac cgtctgcagc atgcgctgcg ccctgttcgt
     3961 gccgcgtcag gacgagattg atctccctcg cgtcatagag ggcatcgaag atcgggatga
     4021 gctgcccacc ggggtatccg aagatcgtcg tgaccttctc ccgcttgagg cactcgacga
     4081 ggatctgtgc acctttcatg cgtcgtcatg tcctttgtta tatgattgaa aaactatcct
     4141 gcaatgatag cacgtcttgg gcccgagaga aaccgctttt tacgtgcgta cctagcagcg
     4201 gggttggcca ctccgggtgc aaaaacccgc ccggaatcac caggcacatc gggcctgagt
     4261 gtgttcatca aaaagatttg tggaaaccac cttggattcc gacaataggg atgtctcgtg
     4321 cttgctttct cgatggtatc gcgatattct gttaccttgg gtgtgatcac tctctggagc
     4381 gtttcagtca tggctgtcag tcgaaaaccg cctgccgcga aaaaaaagcc ggctcccctg
     4441 cccgtccggg aagaggagat cggtgatctc gagacgagct tgcaggagtg ggacaccggc
     4501 ctttttgaca attacgaaaa gaaaatcctc gatctcaaga atctggtgca gatcagcgga
     4561 ctgatcaata cggaaatgaa catgtccgaa atgacccagt ccctcctcta ttcctgccag
     4621 gggatgatgc tcgtccgcag tgtcactctt tttatcctca aaagcattgt ctccaacgtc
     4681 tacgcctacg gcgcttccgt cggatttgcc aacgagagtt ttgccgagga attttcggac
     4741 gaagatccgc tcgtcaccgc actcctcgag cgcaaaaaaa ctctctggat ccgggaaaag
     4801 gctgccgatt cgtcgatcca gacgtcactc ttgcggcttg ccgcgcttga gcccgacctg
     4861 atcatcccaa tgctctacaa ggacaagctg acgggctttc tcgtcgtcgg gcagaaactg
     4921 accggagaga cctttaccag cagtgaggtc gagttcatgg cgaatatggc caacctggca
     4981 gccatcgcca tcgagaatct ccgcctcttc gagatggcaa cgcgtgaccg catgacgaat
     5041 ctctacgtcc accactattt ccagaacagg ctcgaggaag agattgcccg ggcgatccgc
     5101 tatcacaacc atcgcctggc acttctcatg ttcgacatcg accacttcaa gtctgtcaat
     5161 gacacatggg ggcatcaggc gggagatgtc atcatcaagg aaatcgccgg catcatcaag
     5221 gaaaacctgc gcaagctcga ccttccggca cgatatggcg gtgaggagtt cgccgtcatc
     5281 cttcccgaat cggatgtcaa caacgcccga ctgatcgcag aacggatccg ccaggcagtc
     5341 gaaagcaggg tctttgatat cgggaacgga aaacagatcc aggtcaccat ttcggccgga
     5401 gccagcgatc tctcgatggc tgtcgccgcc tccgaacaga tggaaccggc ccagatgtca
     5461 cgcgagagcc agatgcggga agtcctgatc ggccaggccg acaccgcgct ctactgctcc
     5521 aaacacggcg gccgcaacaa ggcgactgtg tttacccccg gcatgaccca ctgagatcgc
     5581 agacaacccc gcccggagca tccgatcctg ccccgacccc cttccggatt attccggcat
     5641 tctcctcgcc aggccttccg ccacggcaca ctcgaccacc gctgcgctga ccgcacccgc
     5701 gacccgcggg tcaaagacgg cgggaatgat ccgcccagct tcccggtccc ggacactgac
     5761 caacccggcc aaagcacccg ccgccgcccg gtacatcgcc ggcgtgatcg accgggacct
     5821 gcaggagaga gcgccacgga atattccggg gaacgccaaa acattgttga tctggttcgg
     5881 ataatccgaa cggccggtgg cgatgacagc atccagcccg tccagcacct cgggcatgac
     5941 ctccgggacc ggattggaga gaccaaagac gatcggtttt ggctgcatgg actggaccat
     6001 ctcccgagac actatccccg gggcactgac accgacaaag
//
LOCUS       DAOSHE010000084         6015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11610_length_6015_cov_4.386409, whole genome shotgun sequence.
ACCESSION   DAOSHE010000084 DAOSHE010000000
VERSION     DAOSHE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11610_length_6015_cov_4.386409"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLD82_03620"
     CDS             <1..939
                     /locus_tag="PLD82_03620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG64505.1"
                     /translation="VEELVRQKYTVRILLRSGSAPKWLKDLPLETVSASYQDGAALRK
                     AIDKVDIIFHVGGVTKALTPAGYLDGNAVPTKALLEAVVASKKKLKRFVLVSSHAMIG
                     PVASPTESSCEDDVPHPVEEYGRSKLTAELVARSYSHLVPITIVRPPTVYGPRDVDCF
                     EIFRMIDKGINLYYGNAKKYTSLIHVEDLVRGMIQAATSKQAVNRAYFLCNDQAVTWK
                     EFQTAVKKVMRKRALTLYVPSFTTRIAVFFGELSMKFTKRPVILNREKAKLGRPSYWI
                     ASNKRARRELRFNPEYSMEEGLAMTWRWYKQNGWLK"
     gene            complement(1102..1734)
                     /locus_tag="PLD82_03625"
     CDS             complement(1102..1734)
                     /locus_tag="PLD82_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64506.1"
                     /translation="MIRYRILLGSLAAILWIAGVLGATSTSPLLRTTVASNTPAAGLA
                     QTNQGSQPAGENFKGFRFGAHRKDVYRILKVRPIRAAHVAEDEEGCEFYSPEKDIQIG
                     DFTVRQGDIYLVFWKDRLMRIRIINTFSGMDDVDIKFFSAMRGALTQKYGNAPSKEQI
                     FTRYDGLYEWKTPTLRVNLQYSMLEYGWIELERELAASLKKSTRINSNDI"
     gene            complement(1785..2666)
                     /locus_tag="PLD82_03630"
     CDS             complement(1785..2666)
                     /locus_tag="PLD82_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOG64507.1"
                     /translation="MRGGFVRWYLVLSMLVSLAGRSVHAEDYSVFLDLHFDIGGVHGS
                     FDRGGSEIDLGGWNTSVGALLYFLSPTGSDNYLGIGVGALYSSDISDWDLSPDVNEIR
                     FTCLKLPVLAGYFYAGEEAYWYLAGGAYARLLNAGSLDPESGSTVYYEDDEFGTGLGL
                     RAEFGFGLIIPGMGAGFGGSLWLEVDLAGPLSESDDSMQAWKFGLGLSLPFFCRGRAS
                     FSGQALAGEPWIPGAFCVSGARVRCWQTGRQAIAGPAVHPLIARSSSKQFLHRCEWPS
                     VRWIFRTDTGIDSSYPF"
     gene            complement(2913..3983)
                     /locus_tag="PLD82_03635"
     CDS             complement(2913..3983)
                     /locus_tag="PLD82_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG64508.1"
                     /translation="MLKKSIFDDPVLKPVRSLVMSSDKFADIAGEHYVLSARLPGMNM
                     FLVSYGPLSDLRGTMVFYMRVLLVLGLAAALVASVVAMFVARGITHGLAGMVAELEGA
                     SGQMRLAANQVALSSASLAENSNNAAASLEEASASLAQVSSQAGLSAENALQTVRLAR
                     MLEDIAQTAGRSMEELSGSMREIAASSSDTFRIISTIDEIAFQTNLLALNAAVEAARA
                     GDAGSGFAVVASEVRALALRSAEAARNTSTLIEATNSRISRGKDLADQTAGAFGDVNA
                     QAIRIVNLVSGIATAAREQADSVLQINRSVASMDQGVQHNAAISEETSSAAKELEQAA
                     DRLSGLVHAINSLTRGGGRDDR"
     gene            complement(3976..4638)
                     /locus_tag="PLD82_03640"
     CDS             complement(3976..4638)
                     /locus_tag="PLD82_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG64509.1"
                     /translation="MSRMSIKSRLVASFTGIIAVLVIALLLLVFVSYKSTLDDELRKN
                     GRVNISELSLELVNWFAPRMQQLETFESLLSSGVCLTPRDLLPAFKMQMTRDAETTSI
                     YYCTRQHYRKGGDFVDGSDWVPDADYDPYSRDWWLQAATNSGTVFVQPYVDAQTKKVV
                     VTLARRVERGGDLLGVIAVDLYLTHLDSIVSNKKHSPNAKTWLVDPRGSLYHPPAAGP
                     CA"
     gene            complement(4930..5691)
                     /locus_tag="PLD82_03645"
     CDS             complement(4930..5691)
                     /locus_tag="PLD82_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64510.1"
                     /translation="MAGISDKDRFIKLVPEDGSTVGNFTLKKSLGWDDDKYWSVRDEL
                     VSDGLISIGRGKGGSVYRLAVSLVGETEVAGKYKSENSLYEPFLKVISTFFIKDKKIK
                     NFVCQKTAYQGKKKTGGKWTRPDIAMVSVDTYSYLPGKFLDITSFELKTWEDFNVAGV
                     FETAAHSKFATKSYYVAYLPDDWDPENVEHVRIKSECERFGVGLMCFSDPEQYETYEI
                     LVEPERRMPDPADMDQFINIQIDDANKRKLSEFLH"
BASE COUNT         1406 a         1806 c         1523 g         1280 t
ORIGIN      
        1 gttgaagaac tcgtccgtca gaaatacacc gtccgcatcc tgcttcgttc cggaagtgcg
       61 ccaaaatggc tcaaggacct tccgctcgaa accgtcagcg cttcctacca ggatggcgcc
      121 gccctccgga aagcgatcga caaggtggat atcatcttcc acgtcggcgg tgtcaccaag
      181 gcactgacac ccgcaggcta tcttgacggg aacgctgttc ccacaaaagc cctcctcgaa
      241 gccgttgtcg cctcgaaaaa aaagctcaag cgttttgttc tcgtgtcctc ccatgccatg
      301 atcggtccgg ttgccagccc caccgagagt tcctgcgagg atgatgtccc gcaccccgtc
      361 gaagagtacg gccgctccaa actgaccgcg gaactcgtcg cccgttccta cagccacctt
      421 gtcccgatca cgattgtccg gccacccacg gtctatgggc cgcgtgacgt ggattgcttc
      481 gagatcttcc gcatgatcga caagggcatc aatctgtatt acggcaacgc gaaaaagtac
      541 acttccctga tccatgtcga agatcttgtc cggggcatga tccaggcggc aacatcgaaa
      601 caggctgtca accgcgccta tttcctctgt aatgaccagg cggtcacgtg gaaagagttc
      661 cagacggccg tcaaaaaggt catgcgaaag cgggccttga ccctctacgt cccgtccttc
      721 accaccagaa ttgccgtctt tttcggggaa ctctccatga aattcaccaa gcgccccgtg
      781 atcctgaacc gtgagaaagc caagctcgga cgccccagct actggatcgc atccaacaaa
      841 cgggccagac gcgaactgcg cttcaatccg gaatattcca tggaagaagg tctggccatg
      901 acctggcgct ggtacaaaca aaacggctgg ttgaaataaa ccctcccctc agtgcccgga
      961 aacgactctt cggcagcgga gttcgtgcaa aaagccacca tggtgcagcc cctgcacgat
     1021 tcatggtgac tttttccatc ttgtcaaaat ttccaagaca attattctgc acaaaccacg
     1081 caattccggc atgggggtat ctcagatatc gttgctgttg atcctggtag acttttttaa
     1141 actggcggcc agctcacgtt ccagctcgat ccagccatac tccagcatgg aatattgcag
     1201 attgaccctg agtgtcggcg tcttccattc gtacaacccg tcgtagcggg taaagatctg
     1261 ttccttgctg ggtgcattcc cgtatttttg tgtcagagcc ccgcgcatcg cagaaaagaa
     1321 tttgatgtca acatcgtcca tgccactgaa ggtattgatg atccggatgc gcatcaggcg
     1381 gtctttccag aacacgagat agatatcccc ctgcctgacc gtaaaatccc ctatttgtat
     1441 atctttttca ggactgtaga actcgcaccc ttcctcatcc tcggcaacat gcgcagcccg
     1501 tattggccgc accttgagaa tacggtacac atccttgcgg tgtgcaccga accggaaccc
     1561 cttgaaattc tccccggcgg gttgcgatcc ctgattggtt tgcgccaacc cggcagccgg
     1621 tgtatttgat gccaccgtcg tccgcagaag gggcgaagtc gatgttgcac caagcactcc
     1681 tgcaatccat aggatggcag ccagactacc aagaagaatc ctgtaccgaa tcattccata
     1741 cctccaatgc gggcatacgc cttgtatcag tgtcggcaat cctgtcaaaa aggataagac
     1801 gagtctatac ccgtatctgt gcggaagatc catcggacag atggccattc gcatcggtgc
     1861 aagaattgtt ttgatgaact ccgtgcgatc agtggatgga cggccgggcc cgctatcgcc
     1921 tgccggcccg tttgccaaca acgcactctc gcaccgctca cacagaaagc ccccggaatc
     1981 cacggttctc ccgcaagtgc ttgcccggaa aaactggccc tgcccctaca aaaaaacggc
     2041 aaggacagac cgaggccgaa cttccaggcc tgcatgctgt catcactctc cgagagcggg
     2101 ccggcaagat ccacctccag ccagagactc ccgccaaaac cggcgcccat cccgggaatg
     2161 atgagaccga acccgaactc ggcccgcaat ccgagtccgg ttccgaattc gtcatcctca
     2221 taatacacgg ttgatccgga ttcgggatcg agactgccgg cgttgaggag acgggcgtat
     2281 gcaccaccgg caaggtacca gtacgcttcc tctccggcat aaaaatagcc ggcaagaaca
     2341 gggagtttga gacaggtaaa gcggatctcg ttgacatcgg gcgaaagatc ccagtcactg
     2401 atatccgagg aatacagcgc gccgactccg atccccaggt agttgtccga cccggtcggt
     2461 gagaggaaat acagcagcgc gcccaccgag gtattccagc cccccagatc gatctcgctt
     2521 ccgcccctgt caaagcttcc atgcaccccg cctatatcga aatgcagatc cagaaaaacg
     2581 gaatagtctt ccgcatggac agaccggccg gccagactga ccagcatgga cagtacaaga
     2641 taccacctga caaaaccacc ccgcatacca gcctcctcta cctgtaccca gtatatcccc
     2701 gcagtgtacc acttgcaaga cagggagctt gcaacagacg gaccacagtc gcgattgata
     2761 cagggagtta caggcttccc gcgagggcaa ggtcaagtgc tttggatact ttttttgatt
     2821 gttttggggt acagcgtccg gccagagggg acgcgcaatg ctcgctgatg ctcatgccat
     2881 gcacagcgca tcacggccgg acgacgccat gttcaacggt catcccgccc gccacctctg
     2941 gtcagggagt tgatggcatg cacaagcccc gagagccgat cggccgcctg ttcgagttcc
     3001 tttgcggccg aggacgtttc ttcgctgatg gccgcattgt gctgcacacc ctggtccatg
     3061 ctggcaacgc tgcgattgat ctgcagtaca ctgtcggcct gttcgcgggc agccgtggca
     3121 attccggaga caagattgac gatgcgtatg gcctgggcgt tgacatcccc gaaggcgccg
     3181 gccgtctggt ccgccagatc cttgccgcgg gaaatacgcg agttggtggc ctcgatcagc
     3241 gtactcgtgt ttctggcggc ctcggccgag cgcagggcca gggcccgcac ctcgctggcc
     3301 acaacggcaa accccgagcc ggcatccccg gcccgcgccg cctcgacagc ggcattgagg
     3361 gccagcaggt ttgtctggaa ggcgatttca tcaatagtac tgatgattct gaaggtgtca
     3421 ctgctggatg ccgcgatctc ccgcatcgag cccgaaagct cttccatcga acgcccggcc
     3481 gtctgcgcga tatcctcgag catgcgggcc agtctgacgg tctgcagcgc gttttcggca
     3541 ctgagaccgg cctgcgaaga aacttgtgcc agtgacgctg acgcttcctc gagtgaggct
     3601 gctgcattgt tggagttttc ggccaaactg gcacttgaca gggcaacctg gtttgccgcc
     3661 agccgcatct gtcccgaagc cccctcgagt tcggcgacca tgccggccag gccatgcgtg
     3721 atcccgcgcg ccacgaacat ggccacaacc gaggcgacga gagctgccgc caggccaagc
     3781 acgaggagca cgcgcatgta aaagaccatc gtgccgcgca ggtcggaaag cggaccatag
     3841 ctgacgagaa acatgttcat gccgggcagg cgggcggaaa gcacgtaatg ctcgccggca
     3901 atgtcggcaa atttgtcgct ggacatgacg agagaacgga caggcttcag cacgggatca
     3961 tcaaaaatgc tttttttaag cacatggtcc tgctgctggt gggtgataaa gtgacccgcg
     4021 cgggtcaacc agccaggtct tcgcgttggg cgagtgcttc ttgttcgaga caatcgaatc
     4081 cagatgggtc agatagagat ccacggcgat gacacccagc aagtcgccac cacgctcgac
     4141 acgtctggca agcgtcacca ctaccttttt ggtctgggcg tccacgtagg gctggacaaa
     4201 gactgtcccg ctgttggtgg cggcctgcag ccaccagtca cgcgaatagg gatcgtagtc
     4261 ggcatcggga acccagtccg agccgtccac aaaatcacca cccttgcggt agtgctgtct
     4321 ggtgcaataa tagatgctgg ttgtctccgc atcccgggtc atctgcattt tgaaggccgg
     4381 caaaagatcc cggggggtca aacaaactcc gcttgaaagc agggattcaa acgtttcgag
     4441 ctgctgcatc cgcggagcaa accagttgac aagttcgagc gaaagctcgg agatattgac
     4501 cctgccgttt ttgcgcagtt cgtcatcaag ggtacttttg tatgacacaa aaacgagcag
     4561 caaaagcgca atgaccagga cagcgatgat gccggtaaag ctcgcgacaa gcctgctctt
     4621 gatcgacata cgtgacatgg cagcctcccc agttcatgac ccaaccgatc cgcagccatg
     4681 atagcactgc aggcgacagt gctcaagagg tttggcctga cgcgcaatcg cttgcacacg
     4741 ttgcctgagt atcagggtgt gccgtgactg tgcaggatgc accgggaagc gctgcaagga
     4801 tgcccacacc ctgtgactgt ttttgatgaa caacgtccgt ccagcggaga gccgacacga
     4861 gcgcgaagga atcgcagatc actgtctgcc ccccgcatgt ggtacagctc atccatgcct
     4921 gaaaaatatt caatgcaaaa actcgctcaa tttccgcttg ttcgcatcgt cgatctggat
     4981 attgataaac tggtccatat cggccggatc aggcatccgt ctttcaggct cgacaaggat
     5041 ttcatacgtc tcgtattgtt cagggtcaga aaaacacatc aggccaacac caaaacgctc
     5101 gcattccgac ttgatacgga catgttcaac attttccgga tcccaatcat cgggcaaata
     5161 cgcgacatag taactttttg tcgcgaattt ggaatgcgcc gctgtctcga aaacaccggc
     5221 aacattgaaa tcctcccatg tcttcaattc aaaactcgtt atatccagaa atttgcccgg
     5281 caaatacgaa tacgtatcaa cggaaaccat ggcgatatcc ggcctcgtcc atttcccgcc
     5341 tgtcttcttt tttccctggt atgctgtttt ctggcaaacg aagtttttta tcttcttgtc
     5401 tttgataaag aatgtgctga tgactttcaa aaatggctcg tacagggaat tctcgctttt
     5461 gtattttccc gcaacttccg tttcgcccac tagcgacaca gcaagcctgt agactgaacc
     5521 acccttgccc cgacctattg aaatcagtcc atcactgaca agctcgtctc gcacactcca
     5581 atacttgtca tcatcccagc caagactctt tttcaacgta aaatttccga cagtgctgcc
     5641 atcttccggc acgagcttga tgaatctatc cttgtcactg attccagcca tattcatcca
     5701 cctccagaat gtgtttcatc gccaacaatc tccgcaaatc aatattctgg ccgcaaagcc
     5761 ggccagagag cggatgccaa tcacgaaggg ctccagaaaa tttcgccctg ttgcaccaga
     5821 gacactcctt ccaatacaat ttcgcgaaca ccgggcacgc ccttgactat gtcattgtac
     5881 gaatagtatg tagcgattgt caacaaggct gaaacagcat atattcgtcc tcgcgttcag
     5941 gataatccac aagccagttc cattccccgc ggctctttgt gaaattgctt gcgtggtact
     6001 ggaacacagg atacc
//
LOCUS       DAOSHE010000085         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11780_length_5947_cov_3.944162, whole genome shotgun sequence.
ACCESSION   DAOSHE010000085 DAOSHE010000000
VERSION     DAOSHE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11780_length_5947_cov_3.944162"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLD82_03650"
     CDS             complement(<1..289)
                     /locus_tag="PLD82_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64511.1"
                     /translation="MERVSCIGYTIGHIASPQPGLVEFEISGAKRYWLVPVPGVRGGS
                     CSGILRTARLPGTGLHPPADVMALRKYIAGGRIARCCNLRHGFLLSVRLAAL"
     gene            398..652
                     /locus_tag="PLD82_03655"
     CDS             398..652
                     /locus_tag="PLD82_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64512.1"
                     /translation="MTDAEREELERLRQENAELRTKVQRGKNLELRVSQKGAVSVYGL
                     NRFPVTLYKEQWERLLDFQEQIREFIRDHENELAVKGKAE"
     gene            complement(735..938)
                     /locus_tag="PLD82_03660"
     CDS             complement(735..938)
                     /locus_tag="PLD82_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64513.1"
                     /translation="MKSTAVRHRRRDDTRFSDHHASAAAYVSFLIDGQKLAQYMIENE
                     LGVSLKQNYKIYVMDTDYFVEEE"
     gene            complement(935..1423)
                     /locus_tag="PLD82_03665"
     CDS             complement(935..1423)
                     /locus_tag="PLD82_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG64514.1"
                     /translation="MKMAESGIDAGSGLVLETDGGHARVRVVSESGACGSGSCASCAG
                     GCHGGLFGETSGRVIQVTNAIGAGVGDLVLLESRPGVRFSAVTLLFVVPLVFFLAGFV
                     LGTEIVPGSDSLAVLLAFGLAGLWYVGLALVHQKKTGRTPVWIAKVIAPGRGDDRGIQ
                     DV"
     gene            complement(1455..2939)
                     /locus_tag="PLD82_03670"
     CDS             complement(1455..2939)
                     /locus_tag="PLD82_03670"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOG64515.1"
                     /translation="MKTRICTSVEAREADLVGFIVHREAGALFGTAWLDRVPGLAGFL
                     GNSLQPTTGAKGRDDVVDGEAGPARRWVDSRTVLYSANWQKHLSPNEAAKTALADLVK
                     TAISLKAASLMIVLDGPGAENVELVEDLARGLGIAAWRFDRYRAKPSPVLENLECVLV
                     TGAPPALADRIAAGLDVAASVNLARGLVNEPGSVMTPEVLAERAASMAREEGLEVEIL
                     DRAGLVAGGYSGILAVGQGSRYEPRMVVLRYRPVGADRHVALVGKGISFDTGGISLKS
                     GKDMHEMKSDMSGAAAVIGAMRTIARKKPGVVVTGILVCAENMIGSAAQRPGDIFTHK
                     NGKSIQVDNTDAEGRLCLIDGLARAGEEGADTIVDIATLTGACIRALGTSLAGVMGND
                     DALTASLVVAGERSGERLWPFPLIEEYAKELETPHADLRNIGTGYAGQITAALFMRPF
                     VPQKAKWAHLDIAGPAYFEKGWKYYAAGATGFGTQLLADWVLGL"
     gene            complement(2936..4336)
                     /locus_tag="PLD82_03675"
     CDS             complement(2936..4336)
                     /locus_tag="PLD82_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64516.1"
                     /translation="MKRHKLQGISTGILISLASILTFSMCAAAGAGGSFPVTGRGPGY
                     TIETRDWPAQTIAGFPEACNRAKAQVYLPQAGPSRARLVLISGWTCGSAWFHHAGAFL
                     AGQCGIEVWAVDRRATLFEDRAGWKADVQAVVNATSNARAALARLQNPQNYLVGKNPA
                     LLEKIGYGVVLEDLDVVVREAGRDGKPVWLGGWSDGVEFVMAYALKEFTELKGGRTRG
                     HQRLAGLVILDENPEWGLFSVVAQRQKLGLALGQVEREQYERRWPAVTVFEALGTGGT
                     TASPFAGLFPAARAARASMTGEALLGWLYDGGGYDSGWSWLVSAGSFDKATPLTGWAH
                     GEKTHLSRVTAIHGAPGGVWEWFYPHMIAADYWEIGSKGFSHDGLRISPDKNNRLPVF
                     MSFSGFNNVAGSIPPQGIQWWLGLTGITNTRMLNFPAYRHADILLSASAESNLWRPMA
                     EWIVAGGRKTERKTQE"
     gene            4432..>5947
                     /gene="malQ"
                     /locus_tag="PLD82_03680"
     CDS             4432..>5947
                     /gene="malQ"
                     /locus_tag="PLD82_03680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG64517.1"
                     /translation="MMNKIHPDLTATRTSGILLHPLSLPGPELCGTLGDEAFALIDWL
                     AAAGQRVWQVLPLGPTGYGDSPYSSFSAFAGNPYMLSLHRLVSLGDLTPDEITPALVS
                     DTRHAGRIDYGALYSEKLPLLRRAAARFFFTVQTDDPRQACFASFCTSEAAWLDDYAL
                     FMAIKEQEGGHSWQDWPEGLRTRRDEAALDNLRASAEYRATCYIQWQFNLQWHALRAH
                     AESKGISILGDAPIFVASDSADVWSNQECFELDADGRSLAVAGVPPDYFSATGQLWGN
                     PLYKWDVMHRNDYRWWKLRLERLLSLVHTVRIDHFRGFAQYWRVPADAPTAITGEWVD
                     GPGAAFFTSLQKNFGTILPLVAEDLGVITPDVVALRLQFSLPGMKIFCFAPWGETEWT
                     DDEGELRPFEEHPYVPTGWEEDCVAYPGTHDNDTFSGWFLSLTTAQQDHVEQWLAPES
                     AGTQSDASRADAVRTAMIRTLIDSQARCVIFQIQDILGLGSEARLNLPGSCSGLN"
BASE COUNT         1217 a         1969 c         1620 g         1141 t
ORIGIN      
        1 acaaagcagc cagccgcaca gacagaagga atccgtgccg cagattgcag catctggcaa
       61 tccttccacc tgcaatatat ttccgcagag ccatcacatc cgcaggaggg tggagacccg
      121 tcccgggcag acgggccgtc ctgaggatac cgctgcacga accaccccgg accccgggaa
      181 caggcacaag ccagtaccgc ttagccccgc tgatttcgaa ttcgaccagc ccgggttgag
      241 ggctggcaat atgaccgatc gtatacccga tacaggaaac ccgttccata gcgcccatcc
      301 gcacgtcata cgtccgacag ggccacgtat ggatttcgtt tgcccgcagg ccagactata
      361 ctataattat agctataacc agagcggagg atacgccatg actgatgccg agcgcgagga
      421 acttgaacga cttcgccagg aaaatgctga actgcggacc aaggtgcagc gaggaaaaaa
      481 tcttgaactc agggtcagcc aaaaaggcgc tgtctcggtc tacggattga accgttttcc
      541 ggtgaccctc tataaagagc agtgggaacg ccttctggat ttccaggagc agatccgtga
      601 atttatccgg gatcatgaaa acgagttggc cgtcaaaggc aaggcggaat aaggactatt
      661 ttttcctaat caaagagctg ctccatacca ggcagctctt tctcttcctg ccaggatccg
      721 cttcctcgtg tgcatcattc ttcttcaaca aaataatccg tatccataac gtatatcttg
      781 tagttctgtt ttagagaaac acccaactcg ttttcaatca tatactgggc cagcttctga
      841 ccatctatca gaaacgaaac gtaagcagca gcggaagcat gatggtctga aaatcgggta
      901 tcatctcgtc tcctgtgcct cacggctgta cttttcacac atcctgtatc cccctatcgt
      961 cgcctcgtcc gggtgcaatc actttggcaa tccagacagg tgtccggcct gttttttttt
     1021 gatgaaccag ggccagtccg acataccaga gtccggccaa gccgaatgcc agcagcacag
     1081 caagggaatc acttcccgga acgatttccg tccccaggac aaatcccgcc aggaaaaaga
     1141 cgagtggcac gacaaagaga agggttaccg cggaaaagcg caccccgggc cggctctcca
     1201 gcaagaccag atcgcccacc ccggcaccaa tggcgttggt cacctggatg acccgccccg
     1261 atgtctcacc aaagagcccg ccatgacacc cgcccgcaca gctcgcacag gaccccgaac
     1321 cgcaggcccc gctctctgaa acaacccgca cgcgcgcgtg cccaccgtcc gtctcaagaa
     1381 caagtcctga cccagcgtcg attcccgact ccgccatttt catcatgcca ccttttttat
     1441 ggttcctttg ttgctcacag gcccaacacc cagtcggcga gcaactgcgt tccgaacccg
     1501 gtcgctccgg ccgcataata cttccagccc ttttcaaaat aggccggccc tgcaatatcc
     1561 agatgcgccc acttggcttt ttggggaaca aagggccgca taaaaagggc cgctgtgatc
     1621 tgtccggcat acccggtccc gatattccgc agatcggcat ggggagtctc caactccttt
     1681 gcgtattcct ctatcaaggg gaaaggccag agccgctcgc ccgatcgctc gccggcgacg
     1741 accagcgagg ccgtcagggc atcgtcattc cccatcacgc cggccaggga agtccccagc
     1801 gcgcggatgc aggcccctgt cagggtggca atatccacga tggtgtccgc accctcctcg
     1861 cccgcacgcg ccagtccgtc gatcagacag aggcggccct cggcgtcagt attgtcaacc
     1921 tgaatggatt ttccgttttt atgcgtaaag atgtctcccg gccgctgggc cgccgatccg
     1981 atcatgtttt cggcgcagac caggattccg gtcaccacca ccccgggctt cttccgggcg
     2041 atcgtccgca tggctccgat cacggcagcc gcaccggaca tgtcgctctt catctcgtgc
     2101 atgtccttgc cggacttgag cgagataccg cccgtatcaa aggagatgcc cttcccgaca
     2161 agggcgacat gccggtctgc accgaccgga cggtagcgca gcaccaccat cctcggttcg
     2221 tagcggctgc cctgaccgac ggccaggatt ccgctatacc cgccagcgac cagcccggcc
     2281 ctgtcgagga tttcgacctc aagcccttct tcgcgcgcca tgctggcggc gcgttctgca
     2341 aggacctcgg gagtcatcac gctccccggc tcgttgacca gtccccgggc gagattcacc
     2401 gaggcagcca catccaggcc ggcagcgatc cggtcagcaa gtgcgggcgg agcaccggtc
     2461 accaaaacgc actcgagatt ttctagaact ggtgagggtt tggcgcggta gcggtcaaac
     2521 cgccaggccg caatccccag cccgcgcgcc aggtcttcga ccaattcgac attttctgca
     2581 ccggggccat ccagtacgat catcaggctc gcggccttga ggctgatcgc ggttttgacc
     2641 agatccgcaa gcgctgtctt ggcggcctcg tttggcgaaa gatgcttctg ccagttcgcg
     2701 gaatacaaga ccgtccggga atccacccaa cgccgggccg gaccggcttc cccgtcaaca
     2761 acatcatcac gccccttcgc gcccgtcgtt ggctggagac tgttccccaa aaaaccggcc
     2821 aagccgggta cccgatccag ccaggcagtc ccgaagagcg ctccggcctc gcgatgcacg
     2881 atgaacccga cgagatcggc ttcccgagct tccacagaag tacaaattcg tgttttcatt
     2941 cttgtgtttt cctttcggtc ttgcgtccgc ccgccacaat ccactcagcc atgggacgcc
     3001 agaggttcga ctcggcgctg gcagagagca ggatatcggc gtgccggtac gccgggaagt
     3061 tgagcatccg ggtattagta atcccggtca agccgagcca ccactggatg ccctgtggcg
     3121 gtatcgatcc ggcaacattg ttgaatccgg aaaaagacat aaaaaccggc agacgattat
     3181 ttttgtccgg agagatcctg agtccgtcat gggagaagcc ctttgaaccg atctcccagt
     3241 agtcggccgc gatcatgtgg ggatagaacc attcccagac tccgccgggc gctccatgga
     3301 tggcggtcac gcgagagaga tgggtctttt ccccatgagc ccaaccggtc aggggcgtgg
     3361 ctttgtcgaa gctcccggcg gaaaccagcc aggaccaacc cgagtcatac ccgcccccgt
     3421 catagagcca gccaagcagg gcctcgcccg tcattgatgc ccgggccgca cgggccgcgg
     3481 gaaagagtcc ggcaaacggg ctggctgtcg tgccccccgt cccgagggct tcaaacacgg
     3541 tcaccgccgg ccagcggcgc tcatattgct cccgctcgac ctgcccgagg gccaagccca
     3601 gtttttgtct ctgggccacg accgaaaaga gcccccattc cgggttctcg tccagaataa
     3661 cgaggccggc caggcgctga tgccctctcg tgcgtccacc cttcagctcg gtaaattcct
     3721 tcaacgcata agccatgacg aattcgacgc cgtccgacca accgcccagc cagaccggct
     3781 ttccgtcccg tcccgcctcg cggaccacga catccagatc ctcgagaaca actccgtacc
     3841 cgatcttttc gaggagggcc ggatttttcc cgacgaggta gttttgcgga ttctgcaagc
     3901 gggccagggc agcccgggca ttgctggttg cattcaccac cgcctgtacg tccgccttcc
     3961 agccggcccg gtcctcgaag agggtcgcac gccggtcaac ggcccagacc tcgatcccgc
     4021 actgtccagc caaaaacgcg ccggcatggt gaaaccaggc cgacccacag gtccagccgg
     4081 aaatcaggac caggcgcgcc cgagagggtc cggcctgtgg caggtagacc tgggctttgg
     4141 cccgattgca tgcctcggga aagccggcga tggtctgtgc cggccaatcc cgcgtctcga
     4201 tcgtgtaccc tgggccacgc cccgtcaccg gaaaagaacc gcccgcaccg gcagcggcac
     4261 acatggaaaa agtcaggatg gaagccagcg atatcaggat ccctgtggag atcccctgaa
     4321 gtttgtgtcg tttcatgata ccaatctact cccgtcattg gtctccggcc acaggggagc
     4381 acacaccttc cctgtcagga gatcttccgt ccattcagac agtatatttt catgatgaac
     4441 aagatacacc ccgatctgac cgcgactcga acaagcggga tactcctcca ccccctctcc
     4501 cttcccggcc cggaactctg cgggaccctg ggtgatgagg cctttgccct gattgactgg
     4561 ctggccgcag ccgggcaacg agtctggcag gtcctgcccc tcggcccgac cggatatggc
     4621 gattcaccat actcatcgtt ttccgctttt gccggcaatc cctacatgct ctccctgcac
     4681 cggctcgtct ctctcggcga tctgactccc gacgagatca ctccggcgct ggtttccgac
     4741 acccgacatg ccgggcgtat cgactacggg gcactctaca gcgaaaagct ccccctcctg
     4801 cgccgggcgg ccgcgcgctt cttttttacg gttcagacgg atgatccacg acaggcctgc
     4861 tttgccagtt tttgcacgtc cgaggccgcc tggctcgacg actacgcact cttcatggcc
     4921 atcaaggaac aggagggtgg ccattcctgg caggactggc ccgagggcct gcgcacccgt
     4981 cgcgatgaag cggcactgga caacctgcgt gcttcggctg aataccgggc gacctgctac
     5041 atccagtggc aattcaacct ccagtggcat gccctgcgcg cccacgccga gtcaaaaggg
     5101 atcagcatcc tcggagacgc gccgattttt gtcgcttcgg actcggccga tgtctggagc
     5161 aatcaggaat gcttcgagct ggacgcggac ggccgctcac tggctgtggc cggggttcct
     5221 cccgactatt tttcggcaac aggccagctc tggggaaatc ccctctacaa atgggatgtg
     5281 atgcaccgga acgactaccg ctggtggaaa ctccgcctcg aacgcctcct ctcgctggtt
     5341 cacaccgtgc gcatcgacca cttccgcggt tttgcccagt attggcgcgt cccggccgat
     5401 gccccgacag ccatcacggg cgagtgggtc gacggtccgg gtgccgcttt cttcacatcc
     5461 ctccaaaaaa acttcgggac gattctcccc cttgtggccg aggatctcgg ggtgattacc
     5521 ccggacgtgg tcgctttacg cctccagttt tccctccccg gaatgaagat cttctgcttt
     5581 gcgccctggg gagaaacgga atggacggac gacgaaggcg agctgcggcc attcgaggag
     5641 cacccctatg tgccgacagg gtgggaggaa gactgcgtgg cctatcctgg cacccatgac
     5701 aacgatacat tctcgggctg gttcctgtcg ctgacgacgg cacagcagga tcatgtggaa
     5761 caatggctgg ccccggaatc cgccggaaca cagagcgatg cttcccgggc cgatgccgtc
     5821 cggacggcga tgatccggac cctgatcgac tcgcaggccc gttgcgtgat cttccagatc
     5881 caggacattc tcgggctggg ttccgaagcc cggctcaacc tgccgggaag ctgcagtggt
     5941 ctcaact
//
LOCUS       DAOSHE010000086         5901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11895_length_5901_cov_4.974512, whole genome shotgun sequence.
ACCESSION   DAOSHE010000086 DAOSHE010000000
VERSION     DAOSHE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_5901_cov_4.974512"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PLD82_03685"
     CDS             complement(<1..1330)
                     /locus_tag="PLD82_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64518.1"
                     /translation="MRVQKEMMRCLLAGLVMVFLAGSVFAETAQDLYRIGKGAFDDGF
                     YDLSRRNLKRLVSEYPDSELADRAQYHIGLGFFRQENYTAAVFEMQTLLQQYPDSGYR
                     LAAGYYIAVSEQKNGRVLESVVLLEKLVAGQKNDPVLPHAWFALGEGCMLLKRYDRAG
                     EAFSHILKTWSIHELVPFARMQYGLSLFRLRDYRGSAREFSLVINTGGRPELVPQAEY
                     WLGESRYWLEEYAEARQRFANILSKWPQSEYAGDSKYRIAFSWLRQDEFETARTNFRE
                     FVAGWPKHAMIPEALLKLGWLAQRGGEYDVALKYYENVIKNFPESGQILAEAAFNAAL
                     CHYKLGNNERAAGMFSDVAKLQSTEEQRILALKSRGDVALVMGKPDLARQAYEEFIKT
                     WPAHELAEEVWYWLGTAYMRTRMFEKARESFLGMQRNFPKSKRLAEVIFLS"
     gene            complement(1327..3807)
                     /locus_tag="PLD82_03690"
     CDS             complement(1327..3807)
                     /locus_tag="PLD82_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG64519.1"
                     /translation="MFEGLTPRAQKIIGLMAQEEAKRLNHDKLVPEHILLGLLKHGQG
                     IAIDTLKNLGIDLDELQRDIENAIKKSGENLMLGEVPPSPRIEKVLRYSAEESKNLGH
                     TYIGTEHLLLGILREDNSKAAMILESRGVKLDKVRNSILKLLGFGTMPKSIGTESVKT
                     PTLDDFSRDLTEIARQGKLDPVIGRQNEIERVVQILSRRKKNNPVLIGEPGVGKTAIV
                     EGLARNIVSGEVPEILLNRRLVTLDLPSLVAGTKYRGEFEERLRNVINEIRKANNIIL
                     FIDELHTLIGAGGAEGAIDAANMLKPALARGELQCIGATTLNEFRKHIERDAALERRF
                     QPILVEEPPVAETVQILHGLKENYETHHKVKYSDEAIDAAANLAHRYIKDRFLPDKAV
                     DLLDEAGAKARLASSTRPATFVELEKEIEILNREKDELVRIQNYEKAADVRDKVKMRK
                     EELKQLTESWRQSINKDVPLIGYEDISRIVSSITGIPLSRLTESESKKLLRMEEDLHK
                     RVIGQEDAIAAVSRAVRRSRTGLKSPKRPTGTFVFLGPTGVGKTELARALAELLFGNE
                     DALIRFDMSEFMEKYSVSRLIGAPPGYVGYDEGGDLTEKVRRRPYSVLLLDEIEKAHP
                     DVFNILLQVMEDGQLTDNLGHTVDFRNTVIILTSNVGAREITKEVSLGFSSFTDDEQD
                     FSDMKEKAMGELRRTFSPEFLNRVDEVVVFHSLTWPHIASILDVMLRELSARLSEKGM
                     ELELSERAREEIIRRGYNRKYGARPLRRTIQREIEDPLAQRFLAGEFKDQSKITIGFQ
                     DGEGFTFMINEAASPKPAIPEEENGSPE"
     gene            3943..>4561
                     /locus_tag="PLD82_03695"
     CDS             3943..>4561
                     /locus_tag="PLD82_03695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG64520.1"
                     /translation="MHNWNPLSWHDFPARQQPDWPDRTKLTRILEILGQRLPLVFSGE
                     VDILRDKIAQAARGKMFLLQGGDCAERFSPRLGEIENLLRVLLQMNMVLMYAAAKPVI
                     KVGRFAGQFAKPRSQPTETINGEEIPNYFGDIVNRLDPDLKSRTPNPKNLLDAYNFSA
                     SILNIVRGLSKGGFADLQQVHSWNEAFILDSPEGQQFRMIAEGISK"
     assembly_gap    4562..4571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4572..5268
                     /locus_tag="PLD82_03700"
     CDS             <4572..5268
                     /locus_tag="PLD82_03700"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG64521.1"
                     /translation="NACGVNSREETATTDFYTSHEALVLDYESGLTRLDPSTGRYYDL
                     SAHMLWLGDRTRQPDGAHVEFLRGIRNPVGIKAGPSMTGDDLCRLLDTLNPANESGRI
                     TLITRFGAPRIGQHLPSLIRTVKTHGYDVLWSCDPMHGNTSTTEHGYKTRDFALVVEE
                     LKAFLEIHKAEGTVAGGVHLELTGENVTECTGGAEGLAAADLSTNYQSACDPRLNARQ
                     ALELAFLIAKEIA"
     gene            complement(5325..>5901)
                     /locus_tag="PLD82_03705"
     CDS             complement(5325..>5901)
                     /locus_tag="PLD82_03705"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOG64522.1"
                     /translation="YSEATNGILRKLDLPDFIYPTAFYVSMRYFSEKYYQYIKRVLNK
                     LIVDGSFKDSLARRTLADEFYKMDDLFNRLIVFSNSVNIRKEKGSLFQSMLQKFKGDL
                     ASKKALGARIATINQELHAVLQDIRDCTVNLQTVLVRIGHDIDSEKPEIVDNLHKIGA
                     AGNVRFLGEMKRSIRESVLFSDIILKVFKDT"
BASE COUNT         1318 a         1727 c         1407 g         1439 t
ORIGIN      
        1 cagaaaggaa aatcacttca gccagccgtt ttgatttcgg aaaattcctc tgcattccta
       61 aaaacgactc ccgggctttc tcgaacatcc gggtgcgcat atacgcggtt cccagccagt
      121 accagacttc ctccgcaagt tcgtgcgccg gccaggtttt gatgaattct tcatatgcct
      181 gcctggccag atccggttta cccatcacca gtgccacatc accccggctt ttcagcgcca
      241 ggatcctctg ttcttcggtg gattgcaatt tggcgacatc cgagaacatt ccggccgctc
      301 tttcgttgtt cccgagttta tagtggcaga gtgcggcatt gaaagctgcc tcggccagga
      361 tctgtccgga ttcgggaaaa ttcttgatga cattttcata gtattttaag gcgacatcgt
      421 attcaccgcc ccgttgtgca agccagccaa gtttgagcag ggcctccgga atcatggcat
      481 gttttggcca cccggcaaca aactccctga aatttgtcct ggccgtctcg aattcatcct
      541 gcctgagcca ggaaaacgcg attctgtatt tagaatctcc ggcatattcg ctttgtggcc
      601 atttggagag tatgttcgcg aatctctgtc tggcttcagc gtactcctcc agccagtatc
      661 tgctttcgcc aagccaatat tcagcctgcg ggacaagttc cggccggcca cccgtattga
      721 tgaccaggga aaattctctc gctgatcccc tatagtcccg caaccggaag agtgaaagac
      781 cgtactgcat gcgcgcaaat ggcaccagtt catggataga ccaggttttc agaatatggg
      841 aaaacgcttc tcccgccctg tcataccgct tgagcagcat gcatccctct cccagtgcaa
      901 accaggcatg cggcaacacc ggatcattct tttgtccggc tacgagtttt tcgagaagga
      961 caacactctc caaaactctt ccatttttct gttcactgac agcgatgtaa tatccggcag
     1021 cgagacggta tcccgaatcc ggatattgct gcaacagcgt ctgcatttca aacacagccg
     1081 ccgtgtaatt ctcctgtctg aaaaacccga gcccgatatg gtactgggca cggtcagcca
     1141 attcactgtc cggatactct gacacaaggc gcttgagatt tcttcttgag agatcataaa
     1201 acccgtcatc aaacgcgccc tttccgatcc ggtagagatc ttgggctgtc tccgcaaaga
     1261 cagatccggc caaaaacacc atgaccaagc ctgcaagcag acacctcatc atctccttct
     1321 ggactctcat tccggactcc cgttctcctc ttccggaata gccggcttcg gggatgcagc
     1381 ttcgtttatc atgaaggtaa agccctcacc atcctggaaa ccgatggtga tctttgactg
     1441 gtccttgaac tccccggcaa ggaatctctg ggccaatgga tcctcgatct cgcgctggat
     1501 tgttcgcctg agcggtcttg ccccatactt cctgttgtag ccccgcctga tgatttcctc
     1561 acgggcccgt tcactcaatt ccagttccat gcctttttca ctcaggcgtg ccgaaagctc
     1621 gcgaagcatc acatccagaa tacttgcgat atgtggccat gtcaacgaat ggaacacaac
     1681 gacctcatcg acacgattca ggaattcggg actgaaggtc cgcctgagtt cccccatggc
     1741 tttttctttc atgtcggaga aatcctgttc atcatccgtg aaggacgaga acccgagtga
     1801 tacttctttt gtgatttcgc gcgcaccgac gttggacgtc agaatgatca cggtattcct
     1861 gaaatcgaca gtgtgtccca gattgtctgt cagctgtccg tcttccatga cctgcaggag
     1921 aatattgaag acatccggat gggctttttc gatctcatcg agcaacaaca cgctgtaggg
     1981 tcttctcctg accttctccg tcaaatcacc gccttcatca tacccgacat acccgggggg
     2041 cgccccgatc aatctgctga ccgaatattt ttccatgaac tcgctcatgt cgaaccggat
     2101 caacgcgtcc tcattcccga agagcagttc ggcaagcgcc ctggccagtt ccgtttttcc
     2161 aacacctgtc ggacccaaaa agacaaaggt tccggtcgga cgtttgggtg acttcaatcc
     2221 cgtgcgcgaa cggcggacag cccggctgac cgccgcaatc gcatcctcct gtccgatgac
     2281 gcgtttgtga agatcctcct ccatgcgcag gagttttttg gattcactct ccgtcagacg
     2341 agaaagcggg atccccgtaa tcgaagagac tatccgcgag atgtcctcgt acccgatcag
     2401 gggaacatcc ttgttgatgg attgacgcca ggattcggtc aactgcttga gctcttcctt
     2461 gcgcatcttg accttgtccc ggacgtcggc ggctttttca taattctgga tccggacaag
     2521 ctcatccttt tccctgttga ggatttctat ctctttttcc agctcgacga atgttgccgg
     2581 acgtgtcgaa cttgccagtc tggctttggc accggcctcg tccagaaggt ctaccgcttt
     2641 gtcggggagg aaccggtcct tgatgtacct gtgcgcaaga ttggcagccg catcaatggc
     2701 ctcatccgaa tatttcacct tgtggtgcgt ctcgtaattc tccttgagtc catgcaggat
     2761 ctggacggtc tcggcgactg gcggttcttc gacaagaatc ggctggaacc ggcgttcaag
     2821 agccgcatcc cgctcgatgt gcttgcggaa ttcattgagg gttgtcgctc cgatacactg
     2881 caactcgccc ctggccaacg ccggcttgag catattggcc gcatcaatgg ccccttcggc
     2941 accgccggcc ccgatcaggg tatgcaattc atcaatgaac aaaatgatat tgttggcctt
     3001 ccgaatttcg ttgatcacgt tccgcaaacg ctcttcaaat tctccacggt atttcgtacc
     3061 ggcaacaagg gagggcaaat ccagggtgac aagccggcgg ttcaacagaa tctcgggaac
     3121 ctcgccactg acaatattcc tcgcaaggcc ctccacgatg gccgttttcc ccacacccgg
     3181 ctctccaatc aggaccggat tgtttttctt ccggcgcgaa aggatctgaa caacgcgctc
     3241 gatctcgttt tgccttccga tcacgggatc cagcttcccc tgacgggcta tctcggtcag
     3301 gtcccggctg aaatcatcca gagtcggtgt cttgacggat tcggttccga tactctttgg
     3361 catggttcca aaaccgagca atttgagaat gctgtttcgg accttgtcga gcttgacgcc
     3421 ccgggattcc aggatcatgg ccgccttgga attgtcttcc cgcaaaattc ccaacagaag
     3481 atgttccgta ccgatgtatg tatgcccgag attcttggac tcctcggcac tgtaccgcaa
     3541 caccttttca atcctcggtg acggaggaac ctcgcccagc atgagatttt cgccgctctt
     3601 cttgatcgca ttctcgatat cacgctgaag ctcatccaga tcaatgccca aatttttgag
     3661 ggtatcgatg gcgatcccct ggccgtgttt cagcaaccca agaagaatgt gctccggaac
     3721 aagtttatcg tgattgagcc tcttggcttc ttcctgagcc atcagaccga tgatcttttg
     3781 tgcccttggt gtcaatccct caaacatcag cggcctcctt tgtatgctat cataaatata
     3841 cttttatcca agactgcaag ccacccaagg taccgcatca gcacttccgg gagtatattc
     3901 ttgtaatatc atctgtcctc aaggatgggt tggaggaagt gcatgcacaa ctggaatcca
     3961 ctctcctggc acgattttcc ggccagacaa cagcccgatt ggccggaccg gaccaaactg
     4021 acccgaatcc tcgagattct cgggcagcgg ctccccctcg tgttttccgg tgaggttgac
     4081 atcctccggg acaaaatcgc acaggccgcc cgcggaaaga tgttcttgct ccagggcgga
     4141 gattgcgcgg agcggttctc cccccgcctt ggcgagatcg agaacctcct gcgcgtcctc
     4201 ctccagatga acatggtctt gatgtacgcc gccgccaaac cggtcatcaa ggtcggacgc
     4261 tttgccggac aatttgccaa accacgctcg caaccaaccg aaaccatcaa cggtgaggag
     4321 attccaaact atttcgggga tatcgtcaac aggcttgatc ccgatctcaa atcaagaacg
     4381 cctaatccga agaatctgct ggatgcgtac aatttcagcg catccatcct caatattgtc
     4441 aggggcctct cgaagggcgg ctttgctgac ctgcaacagg tccattcgtg gaacgaggct
     4501 ttcatccttg acagtcccga ggggcaacag ttccgcatga tcgccgaggg gatttcaaaa
     4561 gnnnnnnnnn ngaatgcctg cggtgtcaac tcgcgtgaag aaaccgcaac aacggatttc
     4621 tatacctccc acgaagccct ggtgcttgat tatgaatccg gactgaccag gctcgatcct
     4681 tcaaccgggc gctactacga cctctcggcc cacatgcttt ggcttggcga tcgcacgcgg
     4741 caacctgacg gtgcccatgt cgaattcctg agaggcatcc gcaatcccgt cggtatcaag
     4801 gcaggtccgt ccatgaccgg agacgatctc tgccgtctcc ttgacaccct caatccggcc
     4861 aatgaatccg gacgaattac gctcataacc agattcggag cacccaggat cggacagcac
     4921 cttccttccc tgatccggac cgtcaaaacg catggctatg atgttctctg gagttgcgat
     4981 cccatgcacg gtaacacaag cacaaccgag catggataca aaacccgtga tttcgcgctc
     5041 gttgtcgagg aactcaaagc gtttctcgaa atccacaagg ctgagggaac agttgccggc
     5101 ggtgtccatc tggaactgac gggagagaat gtgaccgaat gcaccggggg cgccgaagga
     5161 ctggccgctg ccgatctctc gacaaactat cagtcagcct gcgatccccg cctgaatgca
     5221 cgacaggcat tagagctggc tttcctgata gccaaagaaa ttgcctgaaa ccggcaagtc
     5281 ccggcatggg tccggacgga tacttgcagt tccagcgatc tccatcatgt atccttgaag
     5341 actttaagga tgatgtccga gaagaggacc gactccctga tactcctctt catctcaccc
     5401 aaaaatcgga cattcccggc cgcaccgatc ttgtggagat tgtccacaat ctccggcttt
     5461 tcactgtcga tatcatgccc gatccggacc aggacagtct gaagattgac agtgcaatcc
     5521 cggatatcct gcaacacggc atgcaactcc tggttgatgg tcgcaatccg tgcgcccaac
     5581 gcctttttgg acgccagatc acccttgaat ttttgcagca ttgactggaa gaggcttccc
     5641 ttttccttcc ggatgttgac gctgtttgaa aacacaatca atctgttgaa aagatcatcc
     5701 atcttgtaaa actcgtctgc aagtgtccgg cgggccagac tgtccttgaa actcccatcg
     5761 acaatcagtt tgttcaaaac ccgtttgata tactggtaat atttttccga gaagtaccgc
     5821 atgctgacat aaaatgctgt cggatagata aagtccggaa ggtcgagctt tctcaagatg
     5881 ccgtttgtgg cctccgaata c
//
LOCUS       DAOSHE010000087         5870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_11993_length_5870_cov_4.593637, whole genome shotgun sequence.
ACCESSION   DAOSHE010000087 DAOSHE010000000
VERSION     DAOSHE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_5870_cov_4.593637"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..780)
                     /locus_tag="PLD82_03710"
     CDS             complement(43..780)
                     /locus_tag="PLD82_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG64523.1"
                     /translation="MKHVILVVLAALILIPMFLLGYSQEQQEQLGYLKEGPLKWNEWR
                     RDKRPKNIDFNLCNFSNASFNSINLNSVSLKAAIFYKADLSYAKLREANLEAADLQYA
                     NVYNADLFSANLLGANMMGANLSRANLGETDMTYARLNECKLFKADLENAKLYEADLE
                     RVDLRYANLTYSNLARAKLFKADLRFAILNNAILIDAKLTGANLTGADLTRANFSNVD
                     LTAVTFTSNTIFKKATLAGARIQKKRL"
     gene            complement(868..2025)
                     /locus_tag="PLD82_03715"
     CDS             complement(868..2025)
                     /locus_tag="PLD82_03715"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG64524.1"
                     /translation="MEITKRVEALREGDILARSVTSGDGSLVLEKGLRLDSMIIERIR
                     NSGMAGVPVLQILDAQQSQDDSQRKVPQSFTFIGVSQDDVYDSKEQCLKIDSPKALQI
                     HEGMTQFAESILSTISSPQELDMDHIRENIGAIIGLVNTNPAALISLVHLRDLDQYTF
                     MHSVNVSVLSIILGKRLGLESTQLEMLGTGSILHDVGKALVSQEILNKPGKLTDEEFI
                     EMKAHAQRGYEILRKRGADEQVSRIALMHHEKLSGRGYPLGLKAEKVPLLARIVAIAD
                     VYDALTGDRVYKKAMHPSQAFGLMEQDVGQHLDNSLYAIFKSLLGLFPVSSLVLLANG
                     SIARVISQNSSDPARPVVEVVRNRYGQSPKERIVVNTASSAEYAITGIHTE"
     gene            complement(2164..2568)
                     /locus_tag="PLD82_03720"
     CDS             complement(2164..2568)
                     /locus_tag="PLD82_03720"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOG64525.1"
                     /translation="MRRPACIGIAIFLGLLTIMVGCSDKGTKPDGNGTIEDAKNPRID
                     SNGLTFFYTPDAGVTPRSVYLMGSFNGWTPDDAAMQLKAGADGRWSVTVQLDKGRHEY
                     KFVINGKQLPDKISREIENDGFGGIKSYVTVK"
     gene            complement(2634..3179)
                     /locus_tag="PLD82_03725"
     CDS             complement(2634..3179)
                     /locus_tag="PLD82_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64526.1"
                     /translation="MKPVLFIVVFLAGALLSTCASPPSRSGFWGTPSRQEFRRLFREG
                     MQAGKDSEPTWKSIVACIHILEPATVKDLMTHDEPALSVLFLARPDADLHRIISRQLD
                     TASRSFHAVNAAADRLALSGNSFGACLHYAAAACISQQKREQELCLTLAGHYAARLPG
                     PELSGTATAILRSINLRQHHQ"
     gene            3463..4323
                     /locus_tag="PLD82_03730"
     CDS             3463..4323
                     /locus_tag="PLD82_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG64527.1"
                     /translation="MIINHNISSIFANRSVKMNSMSADKNIEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLSRAAKNAEDGISLIQTAEGYLQQSQDVMQRVRELSVQAANGVY
                     SPEDRMMIQVEVSQLIDEVDRIASHAQFNGMNLITGRFGNDTQGQFVGASMWFHLGAN
                     MDQRERVYIGTMTTRGLGVRSLQTNSFVSLSTPNDANRTIGTIDQALMKISKQRADLG
                     AYQNRLEFAVKGILVGYENMQAAESRIRDTDMAQEMSDFVKNQILIQSSTAMLGQANM
                     KPQSVLRLLG"
     gene            4875..5780
                     /locus_tag="PLD82_03735"
     CDS             4875..5780
                     /locus_tag="PLD82_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG64528.1"
                     /translation="MVINHNMSAMFAHRVLQGTMVESDKSIQKLSSGERINRAGDDAS
                     GMAVSEKMRSQIRGLKQAERNVQNGISFLQTAEGYMDETVQILNRVRELAVQSANGIY
                     STDDRMMMQVEVSQLVDEIDRVAKHAQFNGMNLLTGRFGKMQEDYAGIENYAPAKTTA
                     ANKNDAGTYFQVGANMDQREQVFIGDMTAKALKFLDDGNQVTLKLDSPGSANQVIGKI
                     DAAYNIVMKQRADLGAYQNRFEYAVKGIAVAAENIQAAESRIRDTDMAEEMSNFVRGQ
                     ILTQSGAAMLSHANMQGRVALKLLE"
BASE COUNT         1360 a         1500 c         1646 g         1364 t
ORIGIN      
        1 gcgggcggga gcgcgcatcc agaaaaaatg gcagcagttc atctacagcc gttttttctg
       61 gatgcgcgct cccgccagcg tcgctttttt gaagatggta ttcgaggtga atgttacagc
      121 agtcaagtca acattggaga aattggctcg ggtgagatcc gcccctgtca gattggctcc
      181 agtcagcttg gcatcgatca agatggcgtt gttgaggatt gcaaagcgca gatcggcttt
      241 aaaaagcttg gcccgagcaa gattggaata tgtcagattg gcatatcgca gatctacgcg
      301 ttcaagatca gcttcgtaca acttggcatt ttccagatcg gccttgaaaa gtttacactc
      361 gttcaatcgc gcataggtca tatctgtttc gccgaggttg gcccgggaga ggtttgcccc
      421 catcatgttc gctccgagga gattggccga aaagagatcg gcattgtaga cgtttgcgta
      481 ctggagatcg gcagcttcaa gattggcttc acggagcttt gcgtatgaaa ggtctgcctt
      541 gtaaaaaatg gccgctttga gggagacgga attcagattg atgctgttga agctggcatt
      601 tgaaaaattg cagagattga agtctatgtt ttttgggcgc ttgtcccgtc gccactcgtt
      661 ccatttgagc gggccttcct tgagataccc cagttgctcc tgctgttctt gggagtaacc
      721 aagaaggaac atcggaatca ggattagggc tgcaagaaca acgaggatta catgcttcat
      781 ggtggagcct ccagagcggg atctatgcct gcctctcatt gtacaggttt cttgatactt
      841 ctacaataca ctctctgcgc ggcgccttca ctcggtgtga attccggtta tcgcgtattc
      901 ggcagaagat gctgtattga cgacgatccg ttcttttggg ctttgcccgt atctgttccg
      961 gacaacctcg acaaccggtc tggccggatc cgatgaattt tgcgaaatga cccgggcgat
     1021 ggagccattg gccagcagaa ccaggctgga gacgggaaag agaccgagga ggcttttgaa
     1081 gatggcgtag agcgagttgt ccaggtgctg gccaacatcc tgctccatca gaccgaaggc
     1141 ctggcttgga tgcatggctt ttttgtagac tctgtccccc gtcaaagcgt catagacatc
     1201 tgcaatggcc acgattcgtg ccaaaagtgg aactttttca gccttgagtc caagcgggta
     1261 tccccgcccg gataattttt cgtggtgcat cagtgcgata cgtgagactt gttcgtccgc
     1321 accgcgcttt cgcaggattt cgtagcctct ctgggcgtgt gctttcatct cgatgaattc
     1381 ttcatccgtc agctttccgg gcttgttgag gatttcctgg ctcaccaggg cttttccgac
     1441 gtcatgcagg atgcttccgg tccccagcat ttccaactgt gtggattcga gtcccaaccg
     1501 ttttccaaga atgattgaaa gtacggagac attgacggag tgcatgaaag tatattggtc
     1561 cagatcccgg agatggacga ggctgatcag cgctgccggg ttggtgttca ccagaccgat
     1621 gatcgccccg atgttttccc ggatatggtc catgtcgagc tcttgagggc tggagatggt
     1681 agaaagaata ctctcggcga attgtgtcat gccttcatgg atctggagtg cctttgggga
     1741 gtctatcttc aggcactgtt ctttgctgtc gtagacgtca tcctggctga cgccgatgaa
     1801 tgtgaagctt tgaggaacct tgcgttgaga atcgtcttga gattgttgtg catcgagtat
     1861 ctgaagcact ggcacgcccg ccatccccga gttccggatg cgctcaataa tcatgctgtc
     1921 cagtcgcagt cccttttcaa ggaccagact gccatcgccg gaggtgacac ttcgggctaa
     1981 aatatctccc tcccggagtg cttcaacacg cttggtaatt tccatgcttt ctgccatgac
     2041 gtatcaggtc ctgccttgct ggtatggatg ctcccaacca agagtatata tttttttgtt
     2101 gggtttcgcc agcgaaaaaa agcggctgtc tctttggaca accgctgttg aaagatgcga
     2161 aagttatttg acggtcacat aggacttgat gccgccgaag ccgtcatttt caatctcacg
     2221 ggaaatcttg tccggcaatt gttttccatt gatgacgaat ttgtattcgt gacgaccctt
     2281 gtcgagctgg accgtcacac tccagcgacc atcagcccct gctttgagtt gcattgctgc
     2341 atcatccggg gtccaaccgt tgaaactgcc catcagatag acactcctgg gtgtcacacc
     2401 ggcatccgga gtatagaaga atgtcaatcc attgctgtca atcctgggat tcttggcatc
     2461 ctcgatggtg ccattcccgt ccggttttgt ccccttgtct gaacatccaa ccatgatggt
     2521 cagcaatccg agaaaaatgg caattccgat acatgcaggt cttctcatgc agatctcctt
     2581 ggaatttctt ttttttatgg tatcagcaaa tacgggtttt atcaagtatc ggtttactgg
     2641 tgatgttgcc ggaggttgat gctccgcagg atggccgttg cggttccaga gagttcggga
     2701 cccggaaggc gggctgcgta atgcccggcc agggtgagac aaagttcttg ttcccgtttt
     2761 tgctggctga tacaggcagc ggcggcatag tgcaggcagg cgccgaaact gttgccggag
     2821 agtgccagcc gatcggccgc ggcattcacg gcatggaatg atcgggatgc ggtatcgagc
     2881 tgtcgggaga tgatccggtg caagtctgca tcgggacgtg ccagaaagag aacggaaagt
     2941 gctggttcgt catgtgtcat gagatccttg accgttgccg gctcaaggat gtggatgcat
     3001 gcaacaatgg acttccatgt gggctcactg tcctttcctg cttgcatccc ttcccggaac
     3061 agacgccgga attcctgacg ggagggtgtt ccccaaaaac cagaccggga tggtggcgat
     3121 gcgcaagtgg acaagagggc tcctgccagg aacaccacaa tgaagaggac cggtttcatg
     3181 gagtaaaaat gcaaaaaaaa gaggcaggaa agcactcaag tttttttgta atctgccgat
     3241 aatgagtctg gagacagact ggcaagaacg ggagcgggga acatcctctt ctctctagaa
     3301 tgtcggacga ttccaaaaaa acttgagcag tatcgggaaa ttatcacaag aggaggggga
     3361 gattcttcag gcggctcttt tgaacccggc cgactgagca caggaagtac tctcacatcc
     3421 aggcagggag gcctggggag aacattcacg gaggagtgaa acatgatcat caaccacaac
     3481 atcagcagta tttttgccaa ccgcagcgtc aaaatgaaca gcatgagcgc ggacaagaac
     3541 atcgaaaagc tctcttcggg catgcgcatc aatcgcgccg gtgacgatgc ttcgggactt
     3601 gctgtttcag aaaagatgcg cagccagatc cgtggccttt caagggcagc gaaaaacgcc
     3661 gaagatggca tttccctgat ccagacggct gaaggctatc ttcagcagag tcaggatgtc
     3721 atgcagcgtg ttcgcgagct ttctgtccag gccgccaacg gtgtctattc gcccgaagac
     3781 cgcatgatga ttcaggtcga ggtttcgcag ttgatcgatg aagtcgaccg gattgccagc
     3841 catgcccagt tcaacggcat gaacctgatc acgggccgct tcggcaatga cacccagggg
     3901 cagtttgtcg gtgccagcat gtggttccac cttggtgcca acatggacca gcgcgagcgt
     3961 gtctatatcg gtacgatgac gacccgcggt cttggtgtcc gcagtctcca gaccaattcg
     4021 ttcgtctctc tttcaactcc caatgatgcc aaccgcacca tcgggacgat cgatcaagct
     4081 ttgatgaaga tctccaagca gcgcgctgat ctcggtgcgt accagaaccg cctcgagttt
     4141 gccgtcaagg gcatcctcgt cgggtacgaa aacatgcagg ctgccgaaag ccgcatccgc
     4201 gataccgata tggcccagga aatgtctgac ttcgtcaaga accagatcct gatccagtcc
     4261 tccaccgcga tgctcggaca ggccaacatg aagccccagt cggttctgag actgctcggc
     4321 tgatcattga ccagctgatt cccgcctgct cgccgtctca gacggtttgc agcgggagcc
     4381 aggcttgacg tccggagttc ccggcgctgg aaggacaggc acagactgtc cttttcggcg
     4441 tttcgcgaac aatacaggtt ccgtctgaca gagcggaatc gcccggatca cgggagagag
     4501 gaggtggcgg gataccgcaa accacgacgt tctgacaaga acgcggtacc atgttgcgat
     4561 gccaaacgac catgaaaagg cccgacttgg ggtgcatttt cccttccttc agaaacgggg
     4621 attgagtttc ccctgcgtct ggcaggattg gcctccggga attcagtccc ggttgctgaa
     4681 gtcaggagga atatcgccga cggacggaac aagttcgcgc gatacttctg tccattggcc
     4741 agggcccggt caagaggcga tgacctcctc atgatgcacc gtccggcagg gatgccggta
     4801 tctggagacg gtttttcacc ggtgacggcc gaatggaaga ccggaaacca aacggaatca
     4861 aggagggaat ttccatggtt atcaatcaca acatgagcgc tatgtttgcg caccgcgtgc
     4921 ttcagggcac gatggtcgag agcgacaaga gcatccagaa gctctcatcg ggagaacgga
     4981 tcaaccgggc aggggacgac gcctcgggaa tggcagtatc cgagaaaatg cgttcgcaga
     5041 tacgcggcct gaaacaggcg gagagaaacg tccagaacgg gatctccttt ttgcagacgg
     5101 ccgaagggta tatggacgaa accgtccaga ttctcaaccg tgtccgcgaa ctcgcagtcc
     5161 agtctgccaa cggaatctat tcaaccgatg accggatgat gatgcaggtc gaagtgtcgc
     5221 agctcgtcga cgagatcgac agagttgcca aacatgccca gttcaacggc atgaatcttc
     5281 tgaccggtcg ctttggcaaa atgcaggagg actatgccgg gatcgagaat tacgcaccgg
     5341 caaagaccac tgccgccaac aagaatgatg ccgggacgta ttttcaggtc ggcgccaata
     5401 tggatcagcg tgagcaggtc tttatcggtg acatgaccgc caaggcactg aagtttcttg
     5461 atgacggcaa ccaggtcact ctcaagctcg atagtcccgg ttctgcgaac caggtcatcg
     5521 gcaagattga tgccgcctac aacatcgtca tgaagcagcg ggcagacttg ggcgcatatc
     5581 agaaccgctt cgagtacgca gtcaagggta tcgctgtcgc ggccgagaac atccaggctg
     5641 ccgaaagccg catccgcgat accgacatgg ctgaggagat gtccaatttt gtccgtggac
     5701 agattcttac ccagtcgggt gctgcaatgc tctcgcatgc caacatgcag gggcgagtgg
     5761 ctctgaaact gttggagtga cagcagtgta acaggaaccg caccatgaat tgtcagaaac
     5821 gttttggcca gacacagatt catcaccaga ccgcgaggac aataccgctt
//
LOCUS       DAOSHE010000088         5801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12193_length_5801_cov_4.008528, whole genome shotgun sequence.
ACCESSION   DAOSHE010000088 DAOSHE010000000
VERSION     DAOSHE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_5801_cov_4.008528"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLD82_03740"
     CDS             complement(<1..1519)
                     /locus_tag="PLD82_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG64529.1"
                     /translation="MLLTGLSGRPLSGRGIGPAIRARLEKLDIHDMAGLLAHLPRNWQ
                     DRRACTRPEDVRQGDAAWIEGVVTDHIRQRIRGGRSLLKIRLDARGCPLDLVCFNRDY
                     LARTWPVGTRLFVWGSFERELAGLSTAAFEAIRADDPQADLEFGRLVPVYGLTEGVTQ
                     RTMRRIIFDALAGIRGIGHDLPDYLVARRRMRPLDELLLKTHYPGTEAEGLAARDELV
                     YIEFLLFQTGILARRARLGRTHKAHSYSSSDVRETAEAAFGFRLTAGQASAWEDLRRT
                     MSGTEPMQVLLLGDVGSGKTAIAAMAILLAVRSGVQVVMAAPTVILARQLAERMERYL
                     APLGVRTAFLSGGMALDERKRILADLAGSRIDLLSGTHALFHADVIFSRLGLVVIDEQ
                     HRFGVKQRKALRDKGKDADYLAMSATPIPRTLAMALYGDYGSIRIEGIPEGRIPVETH
                     WLQGGQGLEEALSEVEARIRRGEQACFVYPSIGDEDDDSSRAATAAWKRYARTGLA"
     gene            complement(1528..2418)
                     /locus_tag="PLD82_03745"
     CDS             complement(1528..2418)
                     /locus_tag="PLD82_03745"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOG64530.1"
                     /translation="MAEEKNVKVTYFDKKSIACPACSREFQREVLMSGSGRLIAGKLT
                     DELRRLYQPSKKYGRINPLVYPVVVCPDCLYAAFPEDFSRLSEKSTDKMREFTDRRIQ
                     LVMQSVGPVDFSETRDDRAGAASYILATSCYSFFDKSISPTVKRGLCCLRGAWVLNDM
                     VENTEPGEDRDRYAWVSNILYHKAYKFYSDALDLYQKKVETIEGIALGPDTDKNWGYE
                     GFLYTVSQLCLKLGFTEDDLEKRAMSYLGTKRIIAKLFGSGKASKSKPSEILDMSRIL
                     YDKLDAYVKEIEETLGKKFE"
     gene            complement(2435..3412)
                     /locus_tag="PLD82_03750"
     CDS             complement(2435..3412)
                     /locus_tag="PLD82_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOG64531.1"
                     /translation="MHDVVVIGSGPAGLNVAAVASRSGLRCGLVEKHCLAGNITRYPL
                     ALQFRSLATDFELEGMPFVTATAWPQRNEVIRYYQKAARRIENVQFYLRHQATEVIGS
                     DNAFLVKCTNLQVGKEAQIRCRKVVIASGCYDQPRLLQVAGEDLHHVSHYYAEPWACE
                     GANVVIVGGGDSAAEAAIEVAPLARSLVLVYRGVDLHGVRPWVRGSLERLVSAGKLVV
                     HTEARLNEIRDDRVLVQLKTKGEQSIPAEKVYLLTGYRPDDVLLQKAGITIDLDGMVP
                     VYFEETCETNISGMYVAGAILGGTRAGSVGIEKFRSHAQVIVRDIQSKI"
     gene            3564..4187
                     /locus_tag="PLD82_03755"
     CDS             3564..4187
                     /locus_tag="PLD82_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOG64532.1"
                     /translation="MFGRVANARRPLLVLGFMFAVPALVCTILTLIWNHHEGNVLEFS
                     RIDTREKADVILVFGAAIAGHGQPGALLRARINHALALANNGYASNFILTGGVGWGPP
                     AESVVMKRILSEHGIPESRILFETTSHTTREQVEFAVQMMQHHGWKRVLAVSDPFHLY
                     RITRYFEDSGITVLPSPSREIRFTTDEQEEYVRAEVLKLLAWLLLDY"
     gene            complement(4153..5010)
                     /locus_tag="PLD82_03760"
     CDS             complement(4153..5010)
                     /locus_tag="PLD82_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64533.1"
                     /translation="MTTPRRTNFSIHIDNQFVLEMIRRFQSGDLQARASLFKHLWRVI
                     CAWPRFQGFAGREECEWYLDAALERLFPALEHYRDIGRERFDLWFFTVLRNIWVDCLR
                     KQTLVVGTMGDLSQLTDPAVALEEQEADPGVVPLRKLVSPRVWNCLCLRYPELIAEDE
                     IDAFAGRWADGRRKRALVRLIEYARWLARRRRTGLMETLSLIQSRQHRMLKHGREQTT
                     GWENLNREKERTLVRLRSDLVVLQYQTLTRLTGHPVGTIASIIHRARSRIALGQERIN
                     NPGGAKPAA"
     gene            complement(5053..5592)
                     /gene="ruvC"
                     /locus_tag="PLD82_03765"
     CDS             complement(5053..5592)
                     /gene="ruvC"
                     /locus_tag="PLD82_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG64534.1"
                     /translation="MTSKTRRILGIDPGLAIVGYGCIDVSGNRIRPVEYGVLRTGPED
                     ALAVRLSSIHDGCAALFSRLAPDEAAVEELFFGRNVTTGMTVSHARGVILLALHQHGI
                     PHAEYTPMAVKQTVTGDGRADKAGVQGMVQRLLGLDGIPRPDDAADGLAIAICHAQHS
                     PLARLLAQRRGRTPSTKKT"
     gene            complement(5582..>5801)
                     /locus_tag="PLD82_03770"
     CDS             complement(5582..>5801)
                     /locus_tag="PLD82_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG64535.1"
                     /translation="EVLTAPDMFSAVLQAIGEKNIPVENAEVTRIQTSGEPVKVQGDK
                     AKQVMDLLEELEEHDDVTSVSSNFDFDE"
BASE COUNT         1167 a         1887 c         1608 g         1139 t
ORIGIN      
        1 cggccagccc ggtccgtgcg taccgtttcc aggctgccgt cgcggccctg gagctgtcgt
       61 cgtcctcgtc tcctatggaa ggatagacaa agcaggcctg ctcaccgcgc cggatccgtg
      121 cttccacctc ggacaacgcc tcttccaacc cctgcccgcc ctgaagccag tgcgtctcga
      181 cggggatcct gccctccgga atcccttcga tccggatact cccgtaatcc ccataaaggg
      241 ccattgccag agttcggggg atcggggtgg ccgacatggc caggtaatcc gcatccttcc
      301 ccttgtcgcg gagggccttg cgctgcttga ccccaaaacg gtgctgctcg tcgatcacga
      361 ccagtcccag ccgggaaaaa atgacatcgg catggaacaa ggcgtgggtt cctgaaagaa
      421 gatcgatcct cgacccggcc agatccgcca gaatccgttt ccgctcgtcc agcgccatcc
      481 cgccggagag gaaggccgtc cggacaccga gtggtgcgag gtagcgttcc atccgctctg
      541 ccaactggcg ggccaggatg actgtcgggg ccgccatgac gacctggacc ccgctccgga
      601 cagccagcag gatggccatg gcggcgatgg ccgtcttccc ggatccgaca tcgcccagga
      661 gcagaacctg catcggctcg gtaccggaca tcgtgcgccg caaatcctcc catgccgaag
      721 cctggcccgc tgtcagacgg aatccgaatg ccgcctcggc tgtctcgcga acatcgcttg
      781 atgaatagct gtgtgctttg tgcgtccgcc ccagtcgggc ccggcgcgcc aggatcccgg
      841 tctgaaacaa caaaaattcg atatacacga gttcatcccg tgcagccagc ccctcggcct
      901 ccgtcccggg ataatgcgtc ttcaacagca gttcgtccag cggacgcatc cgcctccgcg
      961 cgaccaggta atccggcaga tcatgcccaa tccccctgat gccggcaagc gcatcgaaga
     1021 tgatacggcg catggtccgc tgtgtcacac cctcggtcaa tccatagacg ggcacgagcc
     1081 gcccgaattc aagatccgcc tggggatcat cggcccgaat ggcctcgaag gcagctgtcg
     1141 aaagccccgc cagttcgcgc tcaaagcttc cccagacgaa gagccgggtt ccgactggcc
     1201 acgtccgggc gagataatcc cgattgaaac agacgagatc caggggacag ccccgggcat
     1261 caagcctgat cttgaggaga ctccgtccac ccctgatccg ttgccggatg tggtcggtca
     1321 cgacgccttc gatccaggct gcatccccct gccggacatc ctcgggacgg gtgcaagccc
     1381 gcctgtcctg ccaattccgc gggagatggg caaggagccc cgccatgtca tggatatcga
     1441 gtttttcgag ccgtgcccgg atggccggcc cgataccacg accagaaaga ggacggcccg
     1501 agagtcctgt caggagcatc gggcagtcta ctcgaatttc tttccaaggg tctcttcgat
     1561 ttccttgacg taggcatcca gtttgtcata caggatccgg gacatgtcaa ggatctcgct
     1621 gggtttgctc tttgacgctt ttccgctccc gaagagtttg gcaatgatcc gtttggttcc
     1681 gagataggac atggcccgct tctcgagatc gtcctcggta aacccgagct tgaggcagag
     1741 ctggctaacg gtgtagagaa aaccctcgta cccccagttc ttgtcagtat ccggaccaag
     1801 ggcaatccct tcgatggtct cgaccttctt ctgatagaga tccagcgcgt cggagtaaaa
     1861 tttgtaggcc ttgtgataga gaatattcga aacccatgcg tagcggtcac ggtcctcccc
     1921 gggttcggta ttctcgacca tatcgttcag aacccatgct ccgcgaagac agcagagccc
     1981 gcgcttgacc gtcggcgaga tgctcttgtc aaaaaacgaa taacaactcg tagcgagaat
     2041 atagctcgca gcgcccgcgc ggtcatcccg ggtctcgctg aaatccacgg gaccgacact
     2101 ctgcatcacg agctggattc gcctgtcggt gaattcacgc atcttgtcgg tgctcttttc
     2161 ggaaaggcgc gaaaaatcct cgggaaaggc cgcgtacaga caatccgggc aaacgacgac
     2221 cgggtagacg agcgggttga tccgtccata tttcttgctt ggctggtaga gccggcgcaa
     2281 ctcatcggtc agtttcccgg caatcaaccg gccgctgccg ctcatcagca cctcgcgctg
     2341 gaactcgcgc gaacacgccg gacaggcaat gctctttttg tcaaagtagg ttaccttgac
     2401 gttcttttcc tcggccaccg cttgaactcc cccgtcagat cttggactgg atatcccgta
     2461 caataacttg cgcatgggag cggaattttt caatccccac actcccggct cgggttccac
     2521 caagaatggc ccccgccaca tacatacccg agatattcgt ctcacaggtt tcttcaaaat
     2581 acaccgggac catcccgtcc agatcaatgg tgatcccggc tttttgcaga agaacatcat
     2641 ccgggcggta gcccgtcagg aggtagacct tttcggccgg tatcgactgt tcaccctttg
     2701 tcttcaactg gacaagaaca cggtcatcac ggatctcgtt cagtctcgcc tcggtgtgca
     2761 ccaccagctt gcctgcggaa accagccgct caagactgcc ccggacccag gggcgcaccc
     2821 cgtgcagatc caccccgcga tagacgagca caagactgcg ggcaagagga gcaacctcga
     2881 tggcggcttc ggctgccgaa tctccaccac ccacaatcac gacattcgcc ccctcgcagg
     2941 cccagggctc cgcatagtag tggctgacat ggtgaagatc ctcgccggca acctggagga
     3001 gccggggctg atcatagcat cccgaggcga tcacgacttt ccggcagcgg atctgggctt
     3061 ccttgcccac ctggagattg gtgcatttga ccaaaaacgc gttgtccgac ccgatgacct
     3121 ctgtggcctg gtgcctgaga tagaactgga cgttttcgat ccgtcgggcg gccttctggt
     3181 aatagcggat cacctcattc cgctgtggcc aggcggtcgc ggtcacgaag ggcatccctt
     3241 cgagttcgaa atccgtcgcc agtgaccgga actggagcgc aagcgggtag cgtgtgatgt
     3301 tccccgcgag acaatgcttt tcaaccaggc cacagcgcaa acctgaacgg ctggccacag
     3361 ccgccacatt caaccccgcc gggccgcttc cgatcaccac aacgtcatgc accctctgcc
     3421 tccctgctca ttcctgcagt ctaccctgca gcccgccccc cgtcaacacc cgcccgaaac
     3481 cggattgaaa gacctgaaaa gtggagactc ttctccaaac ggtttggagc caccccggtt
     3541 ctggaacaca ctatatttgg tggatgttcg gtcgcgttgc caatgcccgt cgcccactcc
     3601 tcgtattggg cttcatgttc gcggttcccg ccctggtctg cacgatcctg accctgatct
     3661 ggaatcatca tgagggcaat gtccttgaat tctcgcgcat cgacacacga gagaaagccg
     3721 atgtgatcct cgtttttggt gcggcgatcg ccgggcacgg ccagcccgga gccctcctcc
     3781 gggcccgaat caatcacgcg ctggccctgg ccaacaacgg gtatgcatcc aacttcatcc
     3841 tgaccggagg cgtcggttgg ggtccgccgg cagaatccgt cgtcatgaag agaatcctct
     3901 ccgaacacgg gatccccgag tcccgaatcc tctttgaaac gaccagccat accacacggg
     3961 aacaggtcga attcgccgtg caaatgatgc agcaccacgg ctggaagcgt gtcctcgctg
     4021 tcagtgatcc cttccacctc taccggatca cccggtattt tgaagattcg ggaatcacgg
     4081 tcctcccctc gccctcccgg gaaatccggt tcaccaccga cgaacaggag gagtacgtcc
     4141 gcgccgaggt tctcaagctg ctggcttggc tcctcctgga ttattgatcc gctcctgtcc
     4201 aagggcaatc cgtgaccggg cacgatggat gatgctggcg atcgtcccga ccggatgacc
     4261 ggtcagtcgg gtcagggtct ggtactgcag gacaaccaga tccgaccgca ggcggaccag
     4321 ggtccgctct ttctcccggt tgaggttctc ccaccccgtc gtttgctccc gcccatgctt
     4381 cagcatccgg tgctgccggc tctggatcag ggagagggtc tccatcagac cggtccgccg
     4441 acggcgggcc agccaccggg catattcgat caaccggacc agggcccgct tgcgccggcc
     4501 atcggcccat cgtccggcaa aggcatcaat ctcgtcctcg gcgatcaact ctggataccg
     4561 cagacagaga caattccaga cccggggaga gaccagtttc cgcaggggaa ccacgcccgg
     4621 atcggcctcc tgctcttcta gggccacagc cggatcggtg agttgactga ggtcacccat
     4681 cgtcccgacc acgagtgtct gcttgcgaag gcagtccacc cagatattgc ggagaaccgt
     4741 aaaaaaccag agatcgaacc gttcccggcc gatgtcgcgg tagtgctcca gcgcgggaaa
     4801 aagccgttcg agagcggcat ccaggtacca ctcgcactcc tccctcccgg cgaagccctg
     4861 gaaacggggc caggcacaga tcacccgcca gaggtgttta aagagactgg cccgggcctg
     4921 gagatccccg gactggaaac gccggatcat ctcgaggaca aattggttgt caatgtggat
     4981 cgaaaagttc gttctgcgtg gtgttgtcat atacaggaga gataccagcg ccccgaacgg
     5041 ttccctgttt tttcaggttt tttttgttga aggcgtccgg cccctccgtt gcgccagaag
     5101 ccgggccaac ggagagtgct gggcgtgaca gatagcgatg gccagaccat ccgccgcatc
     5161 gtctggccgg ggaatcccgt ccaggccgag gagtcgttgg accatgccct ggaccccggc
     5221 cttgtcggct cggccatccc cggtcaccgt ctgtttgacg gccatcggcg tgtactcggc
     5281 gtggggaata ccatgttggt ggagagcgag cagaatcacc ccgcgggcat ggctgaccgt
     5341 catccccgtc gtgacattcc tcccgaaaaa aagctcctcc acggccgcct cgtccggagc
     5401 gaggcgtgaa aaaagggcgg cacagccgtc gtggatcgac gaaagccgca ccgccaatgc
     5461 atcctccgga ccggtccgga gaactccgta ttcgaccggt cggatccggt ttccgctgac
     5521 atcgatacag ccatatccga caatggccaa tcccggatca atcccgagaa tccgtctggt
     5581 cttactcgtc aaaatcgaaa ttgctcgaaa ccgacgtcac atcatcatgc tcttcgagtt
     5641 cctcaagaag atccatcacc tgcttggcct tgtctccctg gaccttgacg ggttccccgc
     5701 tggtctggat ccgggtcacc tcggcgtttt cgaccggaat gttcttttca ccaatggcct
     5761 ggagaaccgc tgaaaacatg tccggagcgg tgaggacttc g
//
LOCUS       DAOSHE010000089         5709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12455_length_5709_cov_3.264238, whole genome shotgun sequence.
ACCESSION   DAOSHE010000089 DAOSHE010000000
VERSION     DAOSHE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12455_length_5709_cov_3.264238"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLD82_03775"
     CDS             complement(<1..1013)
                     /locus_tag="PLD82_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG64536.1"
                     /translation="MTHDQKRLLWTLALINFFNYVDRQVVFPLFPLIKDEFSVSDFEL
                     GLLGTVFMLVHSLASLPLGYLADRVHRPRLIAAGVFFWSITTLFCGLAPGFVMLLVLR
                     ALVGIGEASYAPAAAALISDNFGRETRSRAQGVFNIGMFVGGTLGIALGGIIANAAGG
                     WREAFFIVPIPGFILAMLALRMPDKVHHRHEPHVPLKSLLKNHAWLWVLVAGTLVTFA
                     TGAIITWGVEFVRRYLAMDIQTSSLMLGGTLVVSGIIGVVAGSWLADRLATRHSWGRA
                     AVLAGTLTLAGPILFGGIQAGWEGPWFVGLDGTLFADFVSGMGTAGILFFASAFLAGA
                     LMSA"
     gene            complement(1020..2447)
                     /gene="mnmE"
                     /locus_tag="PLD82_03780"
     CDS             complement(1020..2447)
                     /gene="mnmE"
                     /locus_tag="PLD82_03780"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG64537.1"
                     /translation="MLEHPDDTIIAPVTGSGPAAIALIRLSGPDAIRLVAASFRGKGN
                     LAAAASHTLHYGRLVEPDGAGETTLDDVLVSVFRAPHSYTGEETVEISCHGSPVIVRR
                     IMACLVAKGARPAAPGEFTRRAFTNGRMDLAQAEAVADLVSASDEQALLLARRQFEGR
                     LSQEINDLREALIRLSAHAALGLDFADEDTGVEIHEGVRRTLEEVRSAIAAMVASHRA
                     ARLVHDGINLVIAGPPNAGKSSLFNRILRESRAIVSEIPGTTRDVITGNVVIDGRHWI
                     LHDTAGIRSDTDDRIEALGIERTIGMAATADVVVWCDDEQPRAGESTALRDCLRHLAG
                     GTPIVHLWTKADRSPLMQPGSVQQKDNPTRDQYCGTREHPLRVSAVSGEGLDAFFALL
                     GKTADRLAAVEGVSSMISSVRQRDALVRAGHELDAALLALDQGALELAASDIERGARA
                     LAAVTGEVTTDDVLDAVFAGFCIGK"
     gene            2547..5144
                     /locus_tag="PLD82_03785"
     CDS             2547..5144
                     /locus_tag="PLD82_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG64538.1"
                     /translation="MNRMDTDWTSGFPALDKALCGFLPGDNVVWQVEDIADYLPLARR
                     FALASLRQGRRLVYFRFAQHQEVLPLAGQSIICTEPGLPSETAPPRLVELDPSDGFEP
                     FLSSVFSVIEEEGYGVAYIFDCLSELSADWYSDRMLGNFFRLGCPYLFRYDTVAWFAL
                     LGNRHSSHAIDKIHGTAQVILDLYHTADGLFLLPKKAEGRRSPTLFTLHGFTDEACQP
                     VTNSATAARVLSGQPQSWIDFSNRRFGPWTTAFLEAEEFLSRPDKAPVEIRQRFEQLV
                     RMIFTRDQRLLDLARKYLELSHLVSIRRRMIGTGLIGGKSAGFILARAIIQRECLLTL
                     PLEQHDSFFIGSDIYYTYMVDNDCWWVLRKQHDPETVLEGADTARERMLSGTFAPDLV
                     NQFSEMLDYFGQTPLIVRSSSLLEDNYGNAFSGKYESVFCTNQGTPDERLGEFCEAVR
                     RVYASTLSREALLYRADRGLLDSDEQMALLVQRVSGTTTNGCHAPLAAGVAYSFNPYV
                     WKKGIDPAAGFVRLVAGLGTRAVDRSDDDHIRLVALDAPQAFPGDTPQPCQERLDILD
                     LDANRFSCIDFTDISGSIPGVELVASLDQELARRAAYEGRSIARWQLDFEALLEQTEF
                     VPALRTMLRTLEAAYGCPVDTEFALTRDCEGGLHLNLLQCRPFQARRIQTVNMSLDRL
                     NTHNMPILSSGPVVGSGFAGTLARTVLVRPSSYAQLAQSARHEVARSLRRITRDGASG
                     PVLLAGPGRWGSSMASLGVPVSFAEISGVTILVEIVTSTGSAVPEISLGTHFFNDLVE
                     ADMQYLVVTPDDGRARFDEDSFLRYPNALEELFPEYGALAGVIHLIEGVPLVCIVEPH
                     EQRAGVFIS"
     gene            complement(5205..>5709)
                     /locus_tag="PLD82_03790"
     CDS             complement(5205..>5709)
                     /locus_tag="PLD82_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64539.1"
                     /translation="DVCQDVCPLNRAGAVWQGGPAAVRTSRAAGDPGASCPGHGQAGA
                     KSGEARDNPRTAECPDAEVSALFSTANAPGAWIPLTELLELADNEAVRARFAGTALIR
                     AGRRGLVRNAIIVWSNADTGPSRRPASGEACRARVQKLCDDPDPVVAKTARQALRARS
                     SRMYYKK"
BASE COUNT         1150 a         1802 c         1641 g         1116 t
ORIGIN      
        1 gcggacatca gggccccggc cagaaaggcc gatgcaaaaa agaggatacc cgctgtcccc
       61 atcccggaaa caaagtccgc aaagagtgtc ccgtccagtc cgacaaacca cgggccttcc
      121 catccggcct ggattccacc aaagaggatc ggcccggcca gtgtcagggt accagccagg
      181 acagccgccc gtccccacga atgacgcgtg gccagacggt cagccagcca gctcccggca
      241 accacaccga tgatccccga gaccacaagc gtccccccga gcatcaggct tgaggtctgg
      301 atatccatgg ccagatagcg ccggacgaat tcgacgcccc aggtaatgat ggcacccgtc
      361 gcaaaggtga cgagggtccc ggccacgagc acccagagcc aggcatgatt tttgaggagt
      421 gacttgaggg ggacatgggg ctcatgccgg tgatggacct tgtccggcat gcgcagggcg
      481 agcattgcca gaatgaaacc cggaatggga acaataaaaa aggcttcgcg ccagccgccc
      541 gcagcattgg caatgatacc cccaagcgcg atccccagcg ttcctccaac aaacattcca
      601 atattaaaaa ctccctgggc tcgcgagcgt gtctcgcgtc cgaaattgtc cgagatcagg
      661 gccgccgccg ccggagcata gctggcctcc ccgatcccca caagggcccg caggaccaga
      721 agcatgacaa agccaggtgc cagtccgcag aagagggttg taatgctcca gaagaagacc
      781 ccggccgcga tcagtctcgg gcgatggacc ctgtcggcca gatagcccag gggaagactg
      841 gccagggagt gaacaagcat gaagaccgtc cccaagagac cgagttcaaa atcagagact
      901 gaaaactcat ccttgatcag gggaaagagg ggaaagacga cctgccggtc cacatagttg
      961 aaaaagttga tcagggccag ggtccagagg agtcgctttt gatcgtgtgt catgccttct
     1021 cactttccga tgcagaatcc ggcgaagacc gcgtccagta catcgtctgt cgtgacttcg
     1081 cccgtcaccg cggccaaggc tctcgccccg cgctcgatat cactcgcagc cagttcgagc
     1141 gcaccctgat caagggccag gagggctgca tccagctcgt gtccggcgcg gacgagggca
     1201 tcccgctggc ggaccgaact gatcatcgag gaaacaccct caaccgccgc aagccggtcg
     1261 gcagtttttc cgaggagggc aaaaaacgca tccaggccct ccccactgac agccgagacc
     1321 cggagggggt gttcgcgcgt tccgcaatat tgatctcttg ttggattgtc cttttgctga
     1381 acagacccgg gctgcatgag gggcgaccgg tccgccttgg tccagagatg cacaatcggt
     1441 gtgccccccg caagatggcg cagacagtca cgcaaggccg tggattcgcc ggcccgaggc
     1501 tgttcatcat cacaccagac aaccacatcc gccgtcgccg ccatcccgat cgtgcgttcg
     1561 atccccagtg cctcgatccg gtcatcggtg tcactccgga ttccggcggt gtcatgcaga
     1621 atccagtgcc tgccatcaat gacaacgttc cctgtaatga catcgcgtgt tgttccagga
     1681 atctcgctga caatggcacg tgattcgcgc aggatccggt taaagagcga ggactttccc
     1741 gcattgggag gaccggcaat caccagattg atcccgtcat ggaccaggcg ggccgcacgg
     1801 tgactggcga ccatggcggc aatggccgag cggacctctt caagtgttcg gcgcacgccc
     1861 tcgtgaatct cgacccctgt gtcctcatcg gcaaagtcga gacccagcgc agcatgggct
     1921 gaaagccgga tgagtgcttc acgcaggtcg ttgatctcct gcgagaggcg gccctcaaac
     1981 tggcgccggg ccaggaggag tgcctgctca tccgatgccg agacaagatc ggccacagct
     2041 tcggcctggg ccagatccat tcgcccgttg gtaaaagccc ggcgggtaaa ctcgcccggg
     2101 gcagcaggtc gggccccttt agccacgagg caggccatga tcctgcgcac gatcaccgga
     2161 ctcccgtggc aggagatctc gacggtctcc tcacccgtgt acgaatgtgg tgcacggaag
     2221 accgagacaa ggacatcatc aagggtcgtc tcccccgcgc cgtccggctc gacaagccgg
     2281 ccatagtgca gggtatggct ggctgcggcg gcaagattcc ccttgccacg aaagctggct
     2341 gccacaagac ggatcgcgtc cggcccgctg agacggatga gcgcgatggc cgccggcccg
     2401 cttcccgtca ccggggcaat gatggtatca tcggggtgtt cgagcatgcg aaaaatatac
     2461 aacgcgccgg agaagagtta ggcaagactt taatccggga ttctgcggta tactaagcag
     2521 gcagggattc tgctgaggac ggcataatga acaggatgga cacggactgg acaagcgggt
     2581 ttccagccct cgacaaggcg ctctgcggtt ttttgcccgg cgacaatgtc gtctggcagg
     2641 tcgaggacat tgcggattat ctccccctgg cccgcagatt tgcactggcc tcactcaggc
     2701 agggacgcag gctggtctat ttccgttttg cccagcatca ggaggtattg ccgcttgccg
     2761 gacagagtat catctgcaca gaacctgggc tcccgtccga gaccgccccg ccacgcctgg
     2821 tcgaactgga tccctccgac ggatttgaac ccttcctttc atccgtgttt tccgttattg
     2881 aagaagaagg gtacggggtc gcctatatct tcgactgcct ctccgaactc tcagccgact
     2941 ggtacagtga ccggatgctc ggaaattttt tccgcctggg atgcccctac ctcttccgtt
     3001 atgacacggt cgcatggttt gccctcctgg ggaaccggca ctcatcgcac gccatcgaca
     3061 agatccacgg gacggcccag gtcatccttg atctctatca cactgcggat ggcctgtttc
     3121 tgctcccgaa aaaagcggaa ggccgacgtt caccaaccct ctttaccctg catggcttta
     3181 cagacgaggc atgccagcca gtcacaaaca gtgcaaccgc tgcccgggtc ctttcaggcc
     3241 agcctcaatc ctggatcgat ttttccaatc gccggtttgg cccctggaca actgctttcc
     3301 ttgaagccga ggaatttctt tcccggcctg acaaggctcc ggtcgagatc cggcagcgct
     3361 tcgaacagct tgtacggatg atctttaccc gtgaccagcg cctgctggat ctggcacgaa
     3421 aatatcttga gctctcacat cttgtcagca tccgccggcg gatgatcggg accggcctta
     3481 tcgggggaaa gtcggcgggc tttatcctgg cacgggccat cattcagcgc gagtgcctgc
     3541 tgaccctgcc gctggaacag catgactcgt tttttatcgg gtcggatatc tattacacgt
     3601 acatggtcga caatgactgc tggtgggtcc tgcgcaagca acatgacccc gagactgtcc
     3661 ttgagggggc agataccgcc cgggaacgca tgctttcagg aaccttcgcc cctgatcttg
     3721 tcaaccagtt ttctgaaatg cttgactact tcgggcagac tcccctgatt gtgcgctcaa
     3781 gctcgttgct tgaagataat tatggaaatg cgttttctgg taaatatgaa agtgtgtttt
     3841 gcacaaacca ggggacaccg gacgagcggc tgggcgagtt ttgcgaagct gtgcgccggg
     3901 tctatgccag taccctctcg cgtgaggccc tgctctaccg ggcagaccgc gggctccttg
     3961 attctgatga acagatggcc ctcctggtgc aacgcgtctc gggcaccaca accaatggat
     4021 gccatgcccc cctcgccgca ggcgtggcct actcctttaa tccctatgtc tggaaaaaag
     4081 gaattgatcc cgcggccggt tttgtccgtc tcgtggctgg tctgggaacc agggctgtag
     4141 accggagtga cgacgatcac atccgccttg tggccctcga cgcaccacag gcctttcccg
     4201 gcgacacccc ccagccctgc caggaacgcc tggacatcct tgacctggat gcaaacagat
     4261 tttcgtgcat cgattttact gatatctcgg gatccattcc cggagtcgag ctggttgcat
     4321 cccttgacca ggaactggcc cggcgggccg cttacgaggg tcgaagcatc gcccgctggc
     4381 aacttgactt cgaagcactc cttgaacaaa ccgaatttgt cccggcactg cgcaccatgc
     4441 tccggacact tgaagccgcc tacggctgtc ccgtggatac cgaatttgcc cttacgcggg
     4501 actgcgaagg aggactgcac ctgaatctcc tccaatgccg cccattccag gcccggcgga
     4561 tccagactgt caacatgagt cttgaccgtc tcaacacgca caacatgccg atcctctcca
     4621 gcggaccggt cgttggctcg ggattcgcag gaacccttgc gcgaacagtc cttgtccgac
     4681 cttcatcata cgcccagctt gcccagtcag cccgccatga ggtagcgcgt tccctccgtc
     4741 gcatcacccg ggatggcgcc tccgggccag tcctcctcgc gggtccgggc cgctggggaa
     4801 gcagcatggc ctcgctgggg gtccccgtca gctttgccga aatcagcggt gttaccatcc
     4861 tcgttgagat cgtgacgtca accggcagcg cggtccccga aatctccctc gggacgcatt
     4921 ttttcaacga tctggtcgag gctgatatgc agtatctggt tgtcactccg gatgacggcc
     4981 gggcccgctt tgatgaagac tccttcctgc gctatcccaa tgcgcttgaa gaactcttcc
     5041 ccgaatatgg cgcattggcc ggtgtgattc atctcattga gggtgtcccg cttgtctgca
     5101 ttgtcgaacc acacgagcag cgggccggcg tgtttatcag ctgatcctga gagggatcgg
     5161 tatgctgaaa acgtgttttt gtcatggcta tcccggatac gcattcattt tttgtaatac
     5221 atccgggatg agcgtgcccg caatgcctgt cgcgcggtct tggccacaac gggatcggga
     5281 tcgtcacaca atttttggac tcgagcgcgg caggcctcgc cgctggccgg gcggcgggat
     5341 gggcctgtgt ctgcattgct ccatacaata atcgcattgc gcacgagacc acgccgcccg
     5401 gcccggatga gggccgtccc cgcaaagcgc gcccggacgg cctcgttatc ggccagttcc
     5461 aaaagctcag tcaggggaat ccatgctccg ggcgcgtttg ctgtcgaaaa aagcgcgctg
     5521 acctccgcgt cggggcactc tgcggtgcgg gggttgtccc gcgcctcccc ggacttcgca
     5581 cccgcttgcc cgtgtcccgg gcaggatgcg cctggatcgc ccgcggcccg gcttgtccgg
     5641 acagcagccg ggccaccctg ccagaccgcg cccgccctgt tgagcgggca aacgtcctga
     5701 cagacatcg
//
LOCUS       DAOSHE010000090         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12607_length_5661_cov_4.106671, whole genome shotgun sequence.
ACCESSION   DAOSHE010000090 DAOSHE010000000
VERSION     DAOSHE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12607_length_5661_cov_4.106671"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLD82_03795"
     CDS             complement(<1..265)
                     /locus_tag="PLD82_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64540.1"
                     /translation="MKFTAIAASVLAALTLMIGCSSLTRKSPPDIFFGMWRISLTSAD
                     DAAGSDKIVAFEPNGITTQGYMRNGVYTATMTARWTVEGNFMVV"
     gene            complement(400..1032)
                     /locus_tag="PLD82_03800"
     CDS             complement(400..1032)
                     /locus_tag="PLD82_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOG64541.1"
                     /translation="MKKAILLIALLASFSLLTACGEDDATLVVRMVDAPVTVGTYTVE
                     GVYVDISRIDVVKKGSAAATAGVTQIQDGSGVYTVINNANLTNVNLLDLIGGASKLIG
                     SITLDPGDYIQLRLVVGTNSRIKFANDSTFYALKTPSGSTSGIKIKGKGNDPLFSISE
                     GDTSELVFDFDAQASLQASVDKDKFILSPVIKEVRYRSQTKTDAEFDVEK"
     gene            complement(1161..1904)
                     /locus_tag="PLD82_03805"
     CDS             complement(1161..1904)
                     /locus_tag="PLD82_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOG64542.1"
                     /translation="MNDMTGAGLTEKTGYNLPYSLAVGPDNRIYIADTGNNRIVRINS
                     INGSGEVFFGGYGSGINQFNNPQGIAIGYDGKIYIADSGNNRIVRLDDINGTGWTTYG
                     SYGTGTGYFSGPVDIAVNSSGFIMLSDRGNKRVIRINDILGNGWKEYYEGKEVFGVHL
                     FNDSSACFVCRSSPAGSDYLRRLDSNFAPTTFYGAFLNVVGLHINSTGTEFYMAESGN
                     NTIRKTSFSTILATYNTGLNTPMDVFFNE"
     gene            1963..2103
                     /locus_tag="PLD82_03810"
     CDS             1963..2103
                     /locus_tag="PLD82_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64543.1"
                     /translation="MPVLLETMLETEPAEEEGLSEEVSLLAADAELLMVLEAMLMAVE
                     EA"
     gene            2125..2466
                     /locus_tag="PLD82_03815"
     CDS             2125..2466
                     /locus_tag="PLD82_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64544.1"
                     /translation="MALLADGEKETCEDMLKPAWLEVVPHEEESGDAELPGEDGASVS
                     FSMARQPKTRIWTRRPRTAVMAVRRNMGGSREKRFALEYSTFLWMKKNTRQNGRVWKT
                     GREGRVRASGA"
     gene            complement(2447..3622)
                     /locus_tag="PLD82_03820"
     CDS             complement(2447..3622)
                     /locus_tag="PLD82_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOG64545.1"
                     /translation="MHRILFFIIIVFVTIAAACGNENKGSQPEKTVTMVKIDPLLRDT
                     ANLLGGIALPTNSIFAKTASAPAYTAWTNAFNNVWQRTQQPNLDLIKTWASTNLNRAG
                     MPKTLFYPFSGPDILNALAFFPGAEDYILFGMEPAGRIPRPDKDPFQRQMLELGRLQN
                     ALSDILVLNFFQTLHMEVQVTETSYSSVSGILMYFLTRAGMEVIGGRNISIDTNGGIV
                     TNYDPKNARLIPGVEYTFRVRTQNSGQETEASSGTIKRVRYFSLDISDSGLKRNNNFE
                     LFSKSLPSTTTMAKSASFLMHKDFFSRIRTLVLEKSAFILQDDSAVPQRFFLNNTWDV
                     EYFGFYDRPIPLFSNYFQRELLTNMQARSKGRLAFSYGYDHVKGSSNLVLAIKRQKP"
     gene            3787..>5661
                     /locus_tag="PLD82_03825"
     CDS             3787..>5661
                     /locus_tag="PLD82_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG64546.1"
                     /translation="MTKKREFQVGGMHCASCAIRVEEAVRKLSGVEQVAVHLLRGNLW
                     VRGGDELNDEDVMVMVRQLGFSIETEKRENRLADDAKRIEKRARWNAGIAGLCAVPLI
                     GHVMIGHITGNGLGWAPAVETMVQFFLVLPVLWAARDIIRAGFPGIVRPGRAGMDSLV
                     TMGALAAIGYSLAVAGPQWLDGDGWQRMGHSAGHGMADIYFESAAGLLFFILIGRRLE
                     AGARRKTVEAVAELGKSISGSAELVGTDGRTREVAIESILPGDLVLVRAGGKIPADGV
                     IEEGFSGIDESLLTGESLPVERGPGDPVIGGSVNGAGALRVRVRASGEASVLAGIVRL
                     VEEAQGTKAPVQVLADRIARYFVPGVMILALGTGLVWLLAGSAEGVALSRMIAVLVIA
                     CPCALGLAVPAAVMVAGGVGARKGIVVRDAAAWEKASRVDTVVLDKTGTLTLGKAKIF
                     GVACIDGVDEEHVRRMVAALEKASHHPLAGAFEDGSGILCEVQGVQALSGRGLGGRVD
                     GQDLVVGSERVVCADKTEDAGGGSGEEKRFLVNSLKPGKRLLDPRLEEAGRRFARGGA
                     SLVWVVMDGEAVAVYGVRDAIRPEAKMVVGRLQHDGMRVILASGDHREAVRGIAEELG
                     I"
BASE COUNT         1227 a         1233 c         1926 g         1275 t
ORIGIN      
        1 agacgaccat gaagttccct tcgacggtcc agcgtgcggt catggttgca gtgtagacac
       61 cattacgcat atagccctgg gtggtgatgc cgtttggctc aaaggcgacg attttgtccg
      121 atccggcggc atcatccgcc gatgtcaggc tgatgcgcca cattccgaaa aaaatgtctg
      181 gtggggactt ccgggtcagc gatgagcacc caatcatcag ggtgagtgct gccaatacgg
      241 atgcagcgat tgcagtaaat ttcatgggga ctcccgtctg ggcaggaatt gttcacagcc
      301 acccaagtgt agcaccattg tatagagaag tggagagatg atgagaaaaa tgatgaaaaa
      361 gacccgccgg agcgggcctc gagagagcag ggtgttggct catttctcga cgtcaaattc
      421 ggcgtcggtc tttgtctggg agcggtacct gacttccttg atgaccgggc taaggatgaa
      481 cttgtctttg tcgacactcg cctggagtga ggcttgagca tcaaagtcga agaccagttc
      541 gctggtatcg ccttcggaga tcgagaagag cgggtcgttg cccttcccct tgatcttgat
      601 accgctggtt gaaccactgg gcgttttgag agcgtagaag gtgctatcgt tggcgaactt
      661 gatccggctg ttggtgccga ccaccaagcg gagctggatg tagtcgcccg ggtcgagggt
      721 gatgctgccg atcagtttcg atgctcctcc gatgaggtcg agaagattga cattggtcaa
      781 attcgcgttg ttgatcacgg tatagacgcc gctcccgtcc tggatctgtg tgacgcctgc
      841 tgttgcggct gcgctcccct ttttgacaac atcaatccgg gagatatcca catagacacc
      901 ttcgaccgta taggtcccga cagtgactgg cgcatcgacc atgcggacaa ccagagtggc
      961 gtcgtcttca ccgcaggcgg tgagcaggct gaatgatgca agcaaggcta tgaggagaat
     1021 ggcttttttc atgggaaacc tccctgaatg gaatgtggct ggctccgtcc ttcggtgccg
     1081 tacactcagg atatatgccc gaggtgcggg aaggtttcaa aatctccggg cgggagacca
     1141 aacagccgcc caggagggtg tcattcgttg aaaaacacat ccatcggagt attcaagccg
     1201 gtattgtatg ttgcgagaat tgtcgaaaaa ctggtttttc gaatggtatt gtttccgctc
     1261 tcggccatgt agaattccgt gcctgtactg ttgatgtgga gtccgacgac attcaagaac
     1321 gcaccgtaaa acgtggttgg tgcgaagttg gagtcaagcc tgcggagata gtccgatccc
     1381 gcaggggaac ttcggcagac aaagcaggcg gaactgtcgt tgaagagatg gactccgaaa
     1441 acttccttgc cttcataata ttccttccat ccattgccaa ggatgtcatt gatgcggatg
     1501 acccgtttgt tgccccggtc ggatagcatg ataaagccgg atgaattgac ggcaatgtca
     1561 accggcccgg aaaaatatcc cgttccggtc ccgtacgagc cgtaggttgt ccagcctgtt
     1621 ccgttgatat catccagacg gacgatgcga ttgtttccag agtctgcaat atatattttc
     1681 ccgtcatagc cgatagcgat tccctgcggg ttgttgaatt ggttgattcc ggaaccatag
     1741 ccgccgaaga agacctcgcc cgatccgttg atgctgttta tacggacgat acggttgttg
     1801 ccggtgtcag cgatatagat tcggttgtcc ggtccgacag ccaaggagta cggcaggttg
     1861 tatccggttt tttcggtcaa ccccgcgccg gtcatgtcgt tcatgtaaag aatccggttg
     1921 tttgcggtat cagcgatata gatgcctgtt ggcgccgtgc ccgtgcctgt gctgctcgag
     1981 accatgctgg agacggagcc tgccgaggag gaagggcttt cagaggaggt ttcgctgctt
     2041 gctgcagatg ccgagctgct catggtgctg gaggcaatgc ttatggcggt ggaggaagct
     2101 tgagagaagc tggacgagat tagtatggca ctgcttgccg atggggagaa agaaacctgc
     2161 gaggacatgc tcaaacctgc ctggctggag gttgttcccc atgaagaaga gagcggagac
     2221 gcagagcttc cgggagagga cggcgcgtcg gtatcgtttt cgatggcgag gcaaccgaag
     2281 acaagaatct ggacgagaag gcccagaacg gcagtgatgg cagtgcgcag gaacatgggt
     2341 ggctccagag aaaagcggtt tgcacttgag tatagcacat tcctgtggat gaaaaaaaac
     2401 acccgccaga atggacgggt gtggaagacc gggagagaag gacgggtcag ggcttctggc
     2461 gcttgatggc caagacgaga ttagaactcc ccttgacatg atcatagccg tagctgaagg
     2521 cgagccggcc tttggagcgg gcctgcatat tggtcaggag ctcgcgctgg aaatagttgg
     2581 agaagagggg gatcggcctg tcataaaacc cgaaatactc gacatcccat gtgttgttca
     2641 ggaagaagcg ctggggaaca gccgagtcgt cctggaggat gaacgcactt ttttcgagaa
     2701 ccagggtgcg gatgcgggag aagaagtcct tgtgcatcag gaaggatgcc gacttggcca
     2761 tggttgtggt tgacggcaga ctcttggaaa agagttcgaa attgttgttg cgtttgagac
     2821 cggagtcaga gatgtcgagc gagaaatacc ggacacgctt gatggtgccg gaggatgctt
     2881 cggtttcctg cccggagttt tgggtgcgga cgcggaatgt gtattcgaca cccgggatca
     2941 agcgcgcgtt tttcggatca taattggtca cgataccgcc attggtgtcg atgctgatgt
     3001 tgcggccgcc gatgacctcc atgccggccc gggtcaggaa gtacatcagg atgccggaga
     3061 cgctggagta ggatgtctcg gtgacttgga cttccatgtg gagggtctgg aagaaattca
     3121 ggacgagaat gtcggagagg gcgttttgga ggcgtcccag ttcgagcatc tggcgctgga
     3181 agggatcttt gtcgggtcgg gggatccggc cggcaggttc catgccgaag aggatatagt
     3241 cttcagcccc cgggaaaaag gccagcgcat tgagaatgtc cggaccgctg aagggataaa
     3301 agagcgtttt gggcattccg gcgcggttca ggttggttga agcccaggtc ttgatcaggt
     3361 cgagatttgg ctgttgcgtg cgttgccaga cattgttgaa ggcgttggtc caagccgtgt
     3421 aggccggcgc ggaggctgtt ttggcgaaga tggaattggt cggcagagcg atgccaccga
     3481 ggaggttggc tgtgtcacgc aggagggggt caatcttcac catggtgacg gttttttcgg
     3541 gctgactacc cttgttttcg ttcccgcaag cggctgctat ggtgacgaaa acaattatta
     3601 taaagaaaag gatacgatgc atgtgtgttt cttccattta tggcagattt acggggagaa
     3661 cccgcaacgg aaaactagtg agatggggtg gagcggtcaa gggaatgcgg ttcgttccga
     3721 atatggcatg aaaaagcgga aaaccccgac tttaatgcct ataatacctg caggagggat
     3781 atgcaaatga ccaagaaacg ggaatttcag gttggcggaa tgcattgtgc ctcctgtgcg
     3841 attcgggttg aggaggctgt caggaagctc tcaggagtgg agcaggtggc agtgcacctt
     3901 ttgcggggga atctctgggt tcggggcggg gatgagttga atgacgagga tgtgatggtg
     3961 atggttcgcc agctcggctt ttcgatagaa accgaaaaaa gggaaaacag acttgcggac
     4021 gacgccaaaa ggatagagaa gagagccagg tggaatgcag gaatcgccgg attgtgtgcg
     4081 gttcctctga ttggtcatgt gatgatcggt catatcactg ggaacgggtt aggctgggct
     4141 ccggcggtag aaaccatggt gcagtttttt ctagtcctcc cagtgctctg ggcggcgcga
     4201 gacatcatcc gggccgggtt tccggggatt gtgcgaccgg gacgagccgg gatggacagc
     4261 ctggtgacca tgggggcgct ggcggcgatt ggatatagtc ttgcggtcgc cgggccacaa
     4321 tggctggatg gtgacggttg gcagaggatg ggccattccg ccgggcatgg gatggcggac
     4381 atctattttg aatccgctgc cgggctgctg ttcttcattc ttatcggaag gcggctggaa
     4441 gcgggagcaa ggagaaagac ggttgaagcg gtagctgaac tcgggaaaag tatctcgggt
     4501 tcggcggaat tggtcggaac tgatggaaga accagggaag ttgcaatcga gagcattttg
     4561 ccgggggatc tggtcctggt tcgggctggg ggcaaaatcc cggcggatgg cgtgattgag
     4621 gaagggtttt ccggaattga tgaatcgttg ctgactggtg aaagtctgcc tgtggagcga
     4681 ggccctggcg atcctgtcat tggcggaagc gtgaatggtg caggggccct gcgtgtccgt
     4741 gtgcgggcga gcggagaggc cagtgttttg gctgggattg tgcgacttgt tgaagaagca
     4801 caggggacga aagcgccggt acaggtattg gcggaccgga tcgcgaggta ttttgttcca
     4861 ggcgtgatga tcctggcgct tggaacaggt ctggtgtggt tgctcgcagg gagtgccgag
     4921 ggcgtggcct tgtcccggat gatcgcagtg ctcgtgattg cctgtccctg tgctctcggg
     4981 ctggcggttc ctgcggcggt gatggtcgct ggtggcgtcg gagcgcggaa aggcattgtc
     5041 gtgcgggatg ctgccgcatg ggagaaggcg tccagagtgg acaccgttgt gcttgacaag
     5101 acaggaacac tgacactggg gaaggccaag atttttggag tggcgtgtat tgatggagtt
     5161 gatgaagaac acgtgcgcag aatggttgcg gcgctggaaa aagcatcgca tcacccgctc
     5221 gccggagctt ttgaagacgg gtcaggcatt ctatgcgagg ttcaaggggt gcaggcattg
     5281 tcggggagag gccttggcgg tagagtggac ggacaagatc tggttgtggg cagtgagcgg
     5341 gtggtctgtg ctgacaagac cgaggatgcc ggagggggaa gcggggagga aaaaagattt
     5401 ttggtgaaca gcctcaagcc cgggaagcga ctgctggatc cccgattgga agaggccggc
     5461 cggaggtttg cccggggagg ggcgagtctg gtctgggttg tgatggatgg cgaagcggtg
     5521 gctgtatatg gggttcgtga tgccatcagg ccggaggcca aaatggttgt cgggagattg
     5581 cagcatgacg ggatgagagt gattctggcg agcggtgatc atcgtgaagc ggttcggggt
     5641 atagccgaag aattggggat t
//
LOCUS       DAOSHE010000091         5571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12912_length_5571_cov_4.892313, whole genome shotgun sequence.
ACCESSION   DAOSHE010000091 DAOSHE010000000
VERSION     DAOSHE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12912_length_5571_cov_4.892313"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLD82_03830"
     CDS             <1..541
                     /locus_tag="PLD82_03830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64547.1"
                     /translation="DILRASQQSSRMNTLIWSMRRYYRFQFFRHIERDPKTFTPSFWP
                     VDNGGTITSPFGQRFNFFIGQYADHSAVDIAHARGTIIRATAPGVVSRVDNEPGGYGL
                     YVEVKHQDGYITRYAHLDRQEVLTGDLVYQGQIIGRMGHTGLATGDHVHYEVVKDGEY
                     LDPEEFMENKFKPWDLPQP"
     gene            complement(864..2372)
                     /locus_tag="PLD82_03835"
     CDS             complement(864..2372)
                     /locus_tag="PLD82_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG64548.1"
                     /translation="MRAFSSVFRFLALVLTVSVLGGCGSESSFTILHFGDLHGHLHPL
                     KRTEGKTDIESGGIARLIGGLKAAKQAAGPDSLILYNGDTIQGTTFSTFFKGEVSFGL
                     LDGVIDYMVPGVHDFDYGQQNFINLTGKAKFKVLSANIFNKDAKPFTGSDYVIKDFNG
                     IKVGIFGLTVRITEILQPTRNIDGLTFGAVEEAARSMVQMMRSQGVDFIVALTHQGLE
                     EDKKLARAVDGINLIVGGLSHTILTNGFLVFDTWIAHAGYRGEHFGSVTVRYDKGKKQ
                     ILSVTTSVVDVTPEIPADPEVAKVVEKYTKELKSKLDVVVSKTDVLLEGSRNKVRGNE
                     TNLGNLLADVLQRETGAEMCLINSGVIRASINPGPIKIADVLNVLPYPNNVSTVRLSG
                     KTIYRIIEKSAATNPGEGRFLQVSRGLQYKIRGRTLAELKFNGKDIEENRIYTVATSD
                     FLADGGDNYPEFKQERERKNTGITMAEFMIEALKRMPNIDAKVEGRIVRIEG"
     gene            complement(2441..4312)
                     /locus_tag="PLD82_03840"
     CDS             complement(2441..4312)
                     /locus_tag="PLD82_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64549.1"
                     /translation="MKTNSLRTAYSVSVVAAFVVAMALTFVVVYIVTSSLRQDFAVRF
                     GSTYADNVRIRITLRMQRDLNACFQLADSEVVTRWLGDETNPLYRDLAFQTFDTFSRY
                     LENNPGVYAVIESSRNYYLTNVFISKIDRDDPSDTWYFKSLTVPGRFMIDIDYDDVQK
                     KTIFWVNVPVRKQGRLFGIVGTGIDFTAFLDKILYIQQENSFVVLFDEMGMIKGYMDR
                     SLIDKDTVFGLIDQAEERKRLHQEMRSLERTNESGQIRDFPLTKQGTDYMASLAWIPD
                     LGWYALVMIDENQLVQFSQFVPIFVVMMISLVLLLFYIIRMTDRIVLDPLKKLVGIME
                     QVRTGGGHVHAAGTGEYEVARVAEEFDHMISELERQQARIIEQERLAQEFEVVRKMQS
                     AILPKIPYLQDYSLTAVMQPAQEIGGDYYDFYHNRGDRLWYGIGDVSGHGIIPGVIML
                     MTQSAVNTILLMHPDISPRDLSIFVNRALYENIHARLRGNFYMTCYFLTSTRDGQFSF
                     AGSHAEILIYRAGSGECERIKPNGTWLGVFPDISRETVEQEFVLASGDVLVLYTDGIT
                     EAMNEDREEFGMDRLVEVIRRYGREEVETLRERILLDVRAHMAEQQDDMTLFIIRRK"
     gene            4443..5138
                     /locus_tag="PLD82_03845"
     CDS             4443..5138
                     /locus_tag="PLD82_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOG64550.1"
                     /translation="MTEKRPNIIRRLYNWVLHWAETPYGTPALAVNSFAESSFFPIPP
                     DVLLIALDLGKPKRSWWYAFVCTVASVLGGVAGYYIGYGLWASVGKPIVDFYNAQPIF
                     DSLFTTFAEYSFWAVLIAAITPIPYKIFTITAGVVAANAFGSGGFTPDAAGVLAAAQP
                     SATMLGGHTSYFMTFLFASILGRSIRFFGVSALIYFFGERIKGFIDRYFNLLSIAFVV
                     LLIAGFFLISKLS"
     gene            complement(5168..>5571)
                     /locus_tag="PLD82_03850"
     CDS             complement(5168..>5571)
                     /locus_tag="PLD82_03850"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOG64551.1"
                     /translation="LETGEMRFCNAGHNTPWVIARDAVFPLKSGRPGVPLAIFPDEEL
                     VSRNRGYHVERTFLPLDSHILLYTDGLVETRGDDGSEYGDYRLADNLMLHRHEPAARI
                     VSALAEDLVAFHGTEFFEDDVCMVCLRVGQS"
BASE COUNT         1253 a         1624 c         1453 g         1241 t
ORIGIN      
        1 cgatattctc cgggcatcac aacagtcttc gaggatgaac accctgattt ggtcaatgcg
       61 acgctactac cgcttccagt ttttcaggca tattgagcgg gatcccaaaa cctttacacc
      121 atccttctgg cccgttgaca acggtgggac gatcacttcc cccttcgggc aacgctttaa
      181 tttctttatt ggccagtacg cggatcacag tgccgtcgat atcgcgcacg cccgcggaac
      241 gatcatccgg gccaccgcgc cgggtgtcgt cagccgggtg gacaacgagc cgggcggata
      301 cgggttgtat gtcgaggtca agcatcagga cggatatatc acacgctacg cacaccttga
      361 ccgccaggaa gtactgaccg gagatctcgt ctatcaggga cagatcattg gccggatggg
      421 tcacaccggg ttggcgacgg gagaccatgt ccactatgaa gtcgtcaagg acggcgagta
      481 cctggatcct gaggaattta tggagaacaa attcaagccg tgggatctac cccaaccctg
      541 acgggctggc gcgtccaggg gacagcgtcc cggaaatgcg tctggccgat ccggtgtact
      601 ctcccgtata ccgtaaaaaa ggcgctgtcg caatgggcct tcgtccggca gtacatgacg
      661 acactcgacg ggcccgcacc ggcaacggac taccacccaa ggaacgttcc cgacatggaa
      721 caaagccgag gtattcgcag ctgtttgcat gctcgaccgt ggaacctgtc tgtatacggc
      781 ggaacgcgat caatgcttgt tgggcaggcc aaaaaaaccc ggcattgcca tgccgggtcg
      841 cgaattcagt caacctggaa ggatcagcct tcaatcctca ctatccggcc ctcaaccttg
      901 gcgtcgatgt tcggcatgcg cttgagcgcc tcgatcatga actccgccat ggtgataccg
      961 gtgtttttcc gctcccgttc ctgcttgaac tcgggataat tgtccccgcc atcggccaga
     1021 aaatcgctgg tcgcaaccgt atagatccgg ttttcctcga tatccttgcc gttgaacttg
     1081 agttcggcca gggtacggcc ccgaattttg tactgcaagc ccctcgatac ctggaggaag
     1141 cgaccttcac ccggattggt ggcggcactc ttctcgatga tgcgatagat ggtcttcccg
     1201 gagagtcgca ctgtcgaaac attgttcggg taaggtagca cattgaggac atcagcaatc
     1261 ttgatcggac cgggattgat gctggcccgg atcacgccgg aattgatgag acacatctcg
     1321 gccccggtct cccgttgcag gacatcagcc agaagattgc cgagattggt ctcgtttcca
     1381 cggaccttgt tccgggaacc ctcaaggagg acatccgttt tgctgacgac cacgtccaat
     1441 ttgctcttga gttctttcgt gtacttttca acaaccttgg ccacctcggg atcggcgggt
     1501 atctccggcg taacatcgac aacggaggtc gtcactgaca ggatctgctt cttgcccttg
     1561 tcgtaccgga ctgtcaccga gccaaagtgt tcaccccggt acccggcatg ggcaatccat
     1621 gtatcaaaga caaggaatcc gttggtcaga atagtatggg agagtccacc cacaatcaga
     1681 ttgatgccgt cgacggcccg ggcaagtttc ttgtcttctt cgagcccctg atgggtgagt
     1741 gcgacgatga aatcgacccc ctgactacgc atcatctgga ccatgctccg ggccgcctct
     1801 tccacggctc cgaatgtcaa cccgtcaatg ttccgtgtcg gctggaggat ttcggtgata
     1861 cggaccgtca ggccaaagat tccgaccttg atgccattga aatccttgat gacgtagtca
     1921 ctcccggtaa atggtttggc atccttgttg aagatgttgg ccgagagcac cttgaacttg
     1981 gctttgcccg tgagattgat gaagttctgc tgcccgtaat cgaaatcgtg gacaccgggc
     2041 accatgtaat cgatcacgcc atcaagcaat ccaaacgaga cttcgccctt gaaaaatgtg
     2101 gagaaggttg tcccctggat ggtatcaccg ttgtacagga tcaagctgtc aggacccgct
     2161 gcctgcttgg ccgccttgag tccgccgatc aaccgggcaa tcccgccgga ttcgatatcc
     2221 gttttgccct cggtccgctt gagaggatgg agatgaccgt gcaggtctcc aaaatgcaga
     2281 atcgtaaaac tgctttcgga tccacagccg cccaagacgg aaaccgtcag gaccagggca
     2341 agaaatctga aaacagatga aaaagctcgc atgagttacc tccggaaatg ctcatcgtca
     2401 ggagccagta tgccccgaat cagaaaaaaa tggaagaatt ttatttgcga cggatgatga
     2461 agagcgtcat atcatcctgc tgctcagcca tatgggccct gacatcgagg agaatccgct
     2521 cccggagtgt ctcgacctct tccctcccat agcgccggat cacctcgacc agccggtcca
     2581 taccgaactc ctcgcgatct tcattcatcg cctcggtaat cccgtccgtg tagagcacaa
     2641 ggacgtcacc cgatgccaga acgaattcct gctcgaccgt ctcccgcgag atatccggaa
     2701 aaacccccaa ccaggttccg ttcggcttga tccgttcaca ctccccgctc cccgcacggt
     2761 aaatcaggat ctcggcgtgc gagccggcga aactgaactg tccatcgcgg gtcgacgtca
     2821 ggaaatagca ggtcatgtag aaattcccgc gaagacgggc atggatgttc tcgtacaaag
     2881 cacggttgac aaaaatcgag agatcccgcg gcgaaatgtc cgggtgcatg aggaggatcg
     2941 tgttgaccgc tgactgtgtc atcagcatga tcacgccggg gatgatcccg tgaccggaaa
     3001 catccccgat cccgtaccag agccggtccc cccgattatg gtagaaatcg taataatcgc
     3061 ctccgatttc ctgggccggc tgcatgaccg ccgtcagtga atagtcctgg agatagggga
     3121 tcttcggaag gatggcggac tgcatcttgc gcacaacctc gaactcctgg gcaagacgtt
     3181 cctgctcgat gatccgcgct tgctgtcgct caagttcgct gatcatgtgg tcgaactcct
     3241 cggccacccg ggcgacctcg tactccccgg tcccggctgc atggacatgg cctccacccg
     3301 tccggacctg ttccatgatt ccgaccagct ttttgagggg atccaggacg attctgtccg
     3361 tcatgcggat gatgtagaac agaagaagga cgagagaaat catcatcacg acaaaaatcg
     3421 gcacaaattg ggaaaactgg actagctggt tctcgtcaat catgaccagt gcataccagc
     3481 caagatccgg aatccatgcc agggaagcca tataatccgt cccctgtttt gtcaggggaa
     3541 aatcacggat ctggccactc tcgtttgtcc gctcaaggga gcgcatctcc tggtgtaaac
     3601 gcttccgctc ctcagcctgg tcgatcagtc cgaacactgt gtccttgtcg atcagggaac
     3661 ggtccatata ccccttgatc atccccattt cgtcaaaaag aacaacaaat gaattttcct
     3721 gctgtatgta caggatcttg tcgaggaagg ccgtaaaatc gatccccgtc ccgacaatcc
     3781 cgaaaaggcg gccctgtttc cgtaccggca cattgaccca gaagatcgtc tttttctgga
     3841 catcatcgta gtcaatgtcg atcatgaagc ggcccggaac ggtgagtgac ttgaagtacc
     3901 aggtatcgga cggatcatca cggtctatct tgctgatgaa aacattggtc aggtaataat
     3961 tgcgggaact ctcgatgacc gcgtagacgc cgggattgtt ttcgagatag cggctaaagg
     4021 tatcaaaggt ctggaatgcg agatcccggt agagcgggtt ggtctcatca cccagccagc
     4081 gtgtcacgac ttcggaatcc gcaagctgga aacatgcgtt cagatctcgc tgcatccgca
     4141 gggtgatcct gatcctgacg ttgtcagcat aggtggaccc gaaccggacg gcaaaatcct
     4201 gtctgagact ggatgtcacg atgtagacga caacgaatgt cagggccatg gccacaacaa
     4261 aggctgccac aacgctgacg ctgtaagccg ttcgcagact gttggtcttc aagaaactcc
     4321 cgcctctcat ggaagactca tctatcccga ttccaccgct tcattgcacc atggaagtgt
     4381 gcgcgttgtc aagcgttcaa aaagagagta ttcttcagaa acgccaacga tacggagtac
     4441 gtatgaccga aaaacgcccc aacatcatcc gccggctcta caactgggtc ctgcactggg
     4501 ctgaaacacc ctacgggaca ccggctctgg ccgtaaattc cttcgcagaa tcatcatttt
     4561 tccccatccc gccggatgtc ctcttgattg ccctcgatct cgggaaaccg aaacgctcat
     4621 ggtggtatgc cttcgtctgc accgttgcgt ccgtgctggg cggagtcgcc gggtattata
     4681 tcgggtatgg actgtgggcc agtgtcggaa aaccgatcgt cgatttctat aacgcccagc
     4741 cgatctttga cagcctcttc accacctttg ccgagtacag cttctgggct gtcctgattg
     4801 cggccatcac accgattccg tacaagatct tcaccattac ggccggggtg gtcgctgcca
     4861 atgccttcgg cagcggtggt ttcactcccg atgctgccgg cgtcctcgcg gcagcccaac
     4921 cgagtgccac catgctcggg ggacatacct cgtatttcat gacttttttg ttcgcttcca
     4981 tactcggcag gtccatccgc tttttcggag tctccgccct gatctatttt tttggcgaac
     5041 ggatcaaggg ctttatcgac cgctatttca acctgctctc gatcgccttt gtcgtccttt
     5101 tgattgcagg cttctttctt atcagcaaat tgagctgatc cggatccgaa ccgggttccc
     5161 tcccggttca ggactggccg acccgcagac agaccatgca gacatcatcc tcaaaaaatt
     5221 ccgtcccgtg gaatgccacc aaatcctcgg ccagggccga gacaatccgc gccgccggct
     5281 cgtggcggtg cagcatgaga ttgtccgcca aacgataatc gccatactca ctcccatcgt
     5341 cacctctcgt ctcgaccaat ccgtcggtat acagaagaat gtgcgaatcg aggggaagga
     5401 acgttctttc gacgtggtag ccacgattgc gcgaaaccag ctcctcatcg gggaagatcg
     5461 ccaatggcac cccgggccgt cctgacttga gggggaaaac ggcatccctc gcaatcaccc
     5521 acggagtatt gtgaccggca ttgcagaacc gcatctcgcc ggtttcgaga t
//
LOCUS       DAOSHE010000092         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12919_length_5570_cov_3.782049, whole genome shotgun sequence.
ACCESSION   DAOSHE010000092 DAOSHE010000000
VERSION     DAOSHE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12919_length_5570_cov_3.782049"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLD82_03855"
     CDS             complement(<1..662)
                     /locus_tag="PLD82_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64552.1"
                     /translation="MSENKVFKVYDANDVAVSALLLRRGELFFTIGEKHSFSLRGENI
                     IFGAAEVLFGLEHNTLDTRMISVHAREGSDITKIPNANLKKFITLYNIGFNITRVIAR
                     AVQKSNDVIGQLNDRFIKENNLSKRHYITYYNMIKVMGAEAKRRGHPRLEAFIKLKEQ
                     SLAYRKGKLFTQSRKEIQSIEGRRIDEFKTEFPKDAVICKQNDPAEDLFVLNRGQIRV
                     MLG"
     gene            complement(669..1301)
                     /gene="rpe"
                     /locus_tag="PLD82_03860"
     CDS             complement(669..1301)
                     /gene="rpe"
                     /locus_tag="PLD82_03860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG64553.1"
                     /translation="MTVISPSILSADFSRIAEEIRALEAGGADAIHYDVMDNHFVPNL
                     TFGFKFVEDAMKASRLPADVHLMISNPAASLDKWLALKPSALTFHLEAEPDAAPLIRR
                     IKAAGVRAFLSIKPATPVSALSPFLEDLDGVLIMLVEPGFGGQAMLPSTVAKIAELKR
                     LAAHLEIQIDGGIKMDNYKELVSLGANNLVIGSGIFAYSDYAAAIAAFRK"
     gene            complement(1298..2503)
                     /locus_tag="PLD82_03865"
     CDS             complement(1298..2503)
                     /locus_tag="PLD82_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOG64554.1"
                     /translation="MRFPNIPLPGVFRTTLSWVGRRIKRFWKWLTMRKSEVVTERDLW
                     FFHTKRIVVVFAGFLVLSLMTAITFISVWKSGEGMSAVPDVTGMTVIQALSELQDKNL
                     YGTIKTISDISRPSGIILDQEPKGGFFTREKRGVMLFINQPAKSGEVPNLVGKTPTEA
                     KQALLAHASTNYTADLGRIAYGHSETIPAGKIAAQTPSPTSPVVNPLLVDIVVSLGRS
                     HSSVVLPDLKGKDFDEAARWLALNNLSVVPVQVPGGAEGIISSHEPEAGSRVGEGGAV
                     RLSMGGSKKYGVVDFVFPLMLKLSQTTINFTTASATNSSDQDLSIEQLKLKQQRDEER
                     LQRELIREGDEKYQVAITMIRRDGSRGTIFSGEKAPGEHIIQTFSYENEVTIEIAIGG
                     RKFISRKYQ"
     gene            complement(2538..3485)
                     /gene="fmt"
                     /locus_tag="PLD82_03870"
     CDS             complement(2538..3485)
                     /gene="fmt"
                     /locus_tag="PLD82_03870"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG64555.1"
                     /translation="MRFLFFGTPDYTTRYLDCLSGMGAVAGVVTAPDRPSGRGRGLHR
                     PGPAIWAHENGIPCFQPDSPRDPGFPDLLAPLEADLGIVVAYGRILPRPVFALPRLET
                     LNVHFSLLPAFRDASPIEAALLCGASETGVSVQRIVEALDAGDVLDSEMVPIGDGDHF
                     PELFDKLVDAGLLVLRRAVKTLNTGKAVFLPQPEQAATHCGKILPEMRRINWNTPLQD
                     VSNRIRAFAGHRTAWTTLRGSKFLVHRARPGEPTGIPDPAIQTDPGTIVRAAHGDISV
                     VCGDGKVLSLLDVQPENRRRMSATDCINGLRLQTGEQFQ"
     gene            complement(3504..4061)
                     /gene="def"
                     /locus_tag="PLD82_03875"
     CDS             complement(3504..4061)
                     /gene="def"
                     /locus_tag="PLD82_03875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG64556.1"
                     /translation="MLLRSKNMLDIITYGHPDLVTPSAPVQAVDQEIRELVRDMLRTM
                     YRAPGVGLAAPQVDVLKRVCVVGFDEDGQHHELAIINPKITGHFGLETGYDEGCLSVP
                     GIHSIVYRPMGIMVEGIDINEEPLKFHAEGFLARVLQHEIDHLDGILFVDRLGADAKN
                     KVAQSLERLQARTLKKIAGSSRKKS"
     gene            complement(4164..>5570)
                     /gene="priA"
                     /locus_tag="PLD82_03880"
     CDS             complement(4164..>5570)
                     /gene="priA"
                     /locus_tag="PLD82_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG64557.1"
                     /translation="TIGRFASIFPGQIAVLHSQLSPAERIGEWRRLASGAARVAIGAR
                     SAVFAPVGKDSLFIIDEEHESSYKADDCPRYHARQIAFRRTARSGCLILGSATPSLET
                     LWHASKGEITRIPLDERISAHAEQEVVILDMRSQPQGALVAPPLIDAVKEAVSREEQA
                     LFFLNRRGYAPFVTCDDCQTAASCPQCSISLTLHREKGILLCHHCGYQLPFSGHCPAC
                     GGNKIRTGGAGTERVAEFLAKRMGHLRLGRVDSDTIGGSKETLFNLLEDFSHKRLDAL
                     VGTQMVAKGLDFPDLTIVGILAADAELSLPDFRSFERVHTQLVQVIGRTGRAGKKGRA
                     FIQTCLPGEDPIASIVNHDTDGFFKRELERRRECAWPPFSRLIRIVMRSTEPETAYEA
                     INTCTAALSPTLPPEVTLLGPVSCPIEKRANWWRYHFLLKGPSMNVLASVVRKAGLAE
                     GSRGKVYVEVDVDPQSML"
BASE COUNT         1117 a         1462 c         1729 g         1262 t
ORIGIN      
        1 ccgagcatca cccgaatctg tccacggttg agtacaaaga gatcttcggc gggatcgttc
       61 tgtttgcaga tcaccgcatc cttgggaaac tcggtcttga actcgtcgat ccgccgtcct
      121 tcgatggact ggatctcttt acgtgattgg gtaaagagct ttcctttccg gtatgccagg
      181 gattgctcct tgagtttgat gaaggcttca agtcttggat gtccccggcg ctttgcttcg
      241 gcccccatga ccttgatcat gttgtaatac gtgatatagt gccgttttga gaggttgttt
      301 tccttgataa accggtcatt gagctggccg atgacgtcgt tggatttttg gacggcccgg
      361 gcgatcactc tcgtgatgtt gaaaccgatg ttgtagagcg tgatgaattt tttgagattg
      421 gcgttgggga tctttgtgat gtccgaaccc tcacgggcat ggacgctgat catccgggtg
      481 tcgagagtgt tatgttccag gccgaagaga acctcggcag caccgaaaat gatgttttcc
      541 ccacgcagcg agaagctgtg cttttcgcct atggtgaaga agagttcgcc gcgtctgagc
      601 aggagggcgc tgactgcaac atcattggca tcatagacct tgaatacctt gttctcgctc
      661 atggttcctc atttcctgaa ggcagcaatg gcggcggcat agtcactgta ggcaaagatt
      721 ccgcttccga tgacgagatt gtttgcaccc agggatacga gttccttgta gttgtccatc
      781 ttgatcccgc catcgatctg gatctccaga tgtgcagcaa ggcgtttgag ttcggcgatc
      841 ttggcaacag tgctcggaag catggcctgc ccaccgaatc cgggctcgac gagcatgatg
      901 agaaccccgt caagatcctc caaaaaagga ctcaatgcgg agaccggggt ggctggtttg
      961 atggataaaa aagcccggac acctgcggct ttgatccgcc ggatgagggg ggccgcatcc
     1021 ggctcggcct cgaggtgaaa ggtcaaagct gatggtttga gcgccagcca cttgtcgagg
     1081 gatgcggcgg gattcgagat catcagatgg acatcggccg ggagccggga cgccttcatg
     1141 gcatcctcga caaatttgaa accgaaggtc aggttcggga caaagtggtt gtccatcacg
     1201 tcatagtgga tggcatcggc gccgccggct tcaagggcgc ggatctcttc ggcaatccgt
     1261 gaaaaatcgg ctgacaggat ggaaggcgag atgaccgtca ttggtatttc ctcgaaatga
     1321 attttcggcc accgatggcg atctcgatgg tgacctcgtt ttcgtaggag aaggtctgga
     1381 taatgtgttc ccccggtgcc ttttcgccgc tgaaaatcgt tccacgggac ccgtcccggc
     1441 ggatcatggt aatggcgact tgatactttt catcaccttc acggatcaat tcgcgctgga
     1501 ggcgctcttc atcccgttgt tgcttgagtt tgagttgttc gatcgagaga tcctggtcac
     1561 tgctgttggt agcagaagct gtcgtgaaat tgatcgtcgt ctgggagagt ttgagcatca
     1621 gggggaagac aaaatcgaca accccgtatt tcttgcttcc gcccatggaa agacggacgg
     1681 ccccgccttc tccgaccctg ctgccggctt ccggttcgtg cgacgaaatg attccttcgg
     1741 ctccaccggg gacctggaca gggacaacgc tcagattgtt gagagccagc cagcgggcag
     1801 cttcatcgaa atctttgccc ttgagatcgg gaaggacgac cgatgaatgg gatcttccga
     1861 ggctgaccac gatatcgacc agcaaggggt tgacgaccgg tgaggtcgga gagggtgtct
     1921 gggctgcgat cttgccggcc gggatggttt cggagtggcc ataggcgatc cgacccaggt
     1981 cagccgtata gttggtcgag gcatgggcga gaagggcctg ttttgcctcg gtcggagttt
     2041 tgcccacgag gttcgggact tcgccggatt tcgccggctg gttgataaag agcatcactc
     2101 cgcgtttttc gcgggtaaaa aatcctccct tgggttcctg gtcgagaata atccctgaag
     2161 gacgggagat gtcactgatc gtcttgatcg tcccatagag gtttttatcc tggagttccg
     2221 agagagcctg gatgaccgtc atcccggtga catcggggac ggcggacatg ccttctccac
     2281 ttttccagac ggagatgaat gtgatggcag tcatcaggct caggaccaga aagccagcga
     2341 acacgacaac gatgcgcttg gtgtggaaaa accagaggtc tcgttcggtg acaacctcgc
     2401 ttttgcgcat tgtcagccat ttccagaagc gtttgatccg tcggccgacc catgacaggg
     2461 ttgtgcggaa tacgccgggg agggggatat ttgggaatct catgctttct ccttgaatcg
     2521 gggtatgtcg aaatttttta ttgaaattgt tcgcctgttt gcaggcggag accgttgatg
     2581 caatccgtgg cgctcattcg ccgccggttt tccggttgaa catcgagaag cgaaagaact
     2641 ttgccgtcac cgcatacgac actgatgtcc ccgtgtgcag cgcggacaat tgtcccggga
     2701 tctgtctgga tggctggatc aggaattccg gttggttcac cgggccgtgc acggtggacg
     2761 agaaatttgc ttcccctgag ggtcgtccag gctgtccggt gtcccgcgaa agcccgaatc
     2821 cggttggaga catcctgcag gggtgtgttc cagttgatcc gacgcatctc gggaagaatt
     2881 tttccgcaat gtgtggccgc ctgttcgggt tgtggcagaa agaccgcttt cccggtgttg
     2941 agggttttga cggcacgccg cagaaccagg aggccggcgt cgacaagttt gtcaaagagt
     3001 tcggggaagt gatcgccatc cccgatcgga accatttcgc tgtcgagcac atcgccggca
     3061 tccagggctt cgacaattcg ctggacactg accccggtct cgctggcgcc gcagagcagg
     3121 gctgcttcaa tcggtgacgc gtcccggaag gcgggaagca atgaaaaatg gacattgagc
     3181 gtttcaagac ggggaagggc aaaaaccggg cgtggcagaa tccggccata ggccacaaca
     3241 atgcccagat ccgcttccag cggagcaagc aggtcaggaa agccgggatc cctgggtgag
     3301 tccggctgga agcagggaat cccgttttca tgagcccaga tcgcgggtcc gggccggtgc
     3361 aggccgcggc cgcggccact gggacggtcg ggcgctgtga ccacaccggc aacagcgccc
     3421 atcccggaaa gacagtcaag gtagcgtgtt gtgtagtccg gtgttccgaa aaaaagaaaa
     3481 cgcattgtcc ctgtccgcct tgctcaggat ttttttctgg atgaaccggc aatcttcttg
     3541 agggttcggg cctggaggcg ttccagtgat tgtgcgactt tgttttttgc gtctgctccg
     3601 agccggtcaa caaagaggat tccgtcaagg tggtcgattt cgtgttggag aacacgtgcc
     3661 agaaatcctt cggcatggaa tttaagaggc tcttcgttga tatcgattcc ctcgaccatg
     3721 attcccatgg gccggtagac gatactgtga attcccggga ccgagaggca gccctcgtcg
     3781 taccccgttt ccagcccgaa atggcctgtg attttcgggt tgataatggc gagttcgtgg
     3841 tgctgtccgt cttcgtcaaa cccgacaaca cagacccgct tgaggacgtc tacctggggg
     3901 gcagccaatc cgactccggg tgcccggtac atggtacgca acatgtcccg gacgagttcc
     3961 cggatctcct ggtcaaccgc ctgcaccggg gccgaaggag tgaccagatc gggatgcccg
     4021 taagtgatga tgtccagcat gttttttgat cgcagcaaca tatgcgcacc ccttcagacc
     4081 tgcacaagac tatagataaa atatagtaaa tcggagaggc aggggtgtgt gcactgtatt
     4141 cgggaatgcc tggcccgagt tccctagagc atggattgag gatccacgtc gacttcgaca
     4201 tagacctttc cccgactgcc ttcggctaga cccgccttgc gtacaacgga cgcgaggacg
     4261 ttcatggacg gccctttgag cagaaagtgg tagcgccacc agttggcacg tttttcaatg
     4321 ggacaggata cggggccaag caaggtcact tccggtggaa gcgtcgggct gagcgcagca
     4381 gtgcaggtgt tgatggcttc ataggctgtt tcgggttcgg tactgcgcat cacaatccgg
     4441 atcagacggg aaaatggtgg ccaggcacac tcccttcggc gttcgagttc ccttttgaaa
     4501 aagccgtccg tatcatggtt gacgatgctg gcaatcggat cttcgcctgg gaggcaggtc
     4561 tgaatgaagg cccgtccttt tttcccggcc cggccggtcc tcccgatcac ttgtacgagt
     4621 tgggtatgga cccgctcaaa actgcggaag tcgggaaggg agagctccgc atcggcagcc
     4681 aggatcccga cgatggtcag gtcagggaag tccagtccct tggccaccat ctgggttccg
     4741 accagggcgt ccagccgttt gtgcgagaag tcctcgagga ggttgaagag ggtttccttt
     4801 gagcctccga tcgtgtccga gtcgacccgc cccagtcgga gatgccccat ccgctttgcg
     4861 aggaattccg ctacccgctc cgtgccggct cctccggtcc ggattttgtt cccgccgcaa
     4921 gccgggcaat gtccggaaaa gggaagctga tatccgcagt ggtgacagag caggattccc
     4981 ttttcacggt gcagtgtcag cgagatgctg cactgcggac aactggcggc agtctggcag
     5041 tcatcgcagg tgacgaacgg agcgtagccg cgccgattga gaaaaaagag ggcctgctct
     5101 tccctggaaa cggcttcctt gacggcatcg atcaggggtg gcgccaccag ggcgccctgc
     5161 ggctggctcc gcatgtcgag gatgacgact tcctgttcgg catgggcgga gatccgttcg
     5221 tcaagaggga tacgagtgat ctcgcctttg gatgcgtgcc agagggtttc gagggagggg
     5281 gtggcgcttc ccaggatcag gcagccggag cgggccgtcc ttcggaaggc gatctggcgg
     5341 gcgtggtagc gcgggcagtc gtcggccttg tagctcgact cgtgttcctc gtcgatgatg
     5401 aagagactgt cctttccgac tggtgcgaac acggcggagc gggccccgat ggcgacccgt
     5461 gcggcaccgc ttgcgagtcg gcgccattct ccgatccgtt cggccggcga gagttgcgaa
     5521 tgaagaacgg cgatctgtcc ggggaagatg ctggcaaacc ggccgatggt
//
LOCUS       DAOSHE010000093         5553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_12974_length_5553_cov_3.951619, whole genome shotgun sequence.
ACCESSION   DAOSHE010000093 DAOSHE010000000
VERSION     DAOSHE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12974_length_5553_cov_3.951619"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLD82_03885"
     CDS             <1..986
                     /locus_tag="PLD82_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG64558.1"
                     /translation="LSQPVQSIKSWCGIEQIDRCVAVSEDISGLLDRVRDTTGVSHDL
                     TADKFGELRKFVLACPQEEMASRWGLDGNRAVTIRIGLLVIGMLFEQSGAGRIIFPPI
                     HLTSAVVDALVRTEGGFPDLLRDGFEGEILEGAHAIGRKFKYDEAHARECERIAGLLY
                     DQLAQSFQPRERLLVRVAAILHDIGMYIGSKGHHRYSAELVAASDILGLSACEVQEVS
                     WTVRFHRKGLPGGTAIGYKAMDRSARLAVFRMAAILRLADALSEPGLPRIEAISIRFG
                     ESAAILSVGLEGKRYEFLEIMRVLLNNKKQYFEHYFGTDLVLERLRTVDGP"
     gene            983..3079
                     /gene="ppk1"
                     /locus_tag="PLD82_03890"
     CDS             983..3079
                     /gene="ppk1"
                     /locus_tag="PLD82_03890"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOG64559.1"
                     /translation="MSGRFFNREWSWLAFNSRVLDESRRPGTPLLERVRFLSISSSNL
                     EEFFMIRVAALKRLGAMHVPARSPDGLEPREQLAGIRERVLGMVRLQYRVFHEELLPG
                     LASHGIRIMLQKEEIEPWLDELFILYRDSIRPVLTPISVGGTHPFPNLVTGRLYLAVS
                     LGVDEARTDQIEKSRLSFVEIPVRAHGRFLRLEEGCFVPLELVVRTFLPELFAGYSVE
                     GAWLIKVTRDADLDIEQDAAGDLLTLIENKLTRMHHRSVVRLEYEQGMDETMRLLLAR
                     QLGVGEADQYGIEGLFTLHDLAGFVERIPRPGLVWPEAQAVLVRDFAVDGAFAAIATR
                     DRLLLHPWHSYKPVIDFINQAADDPAVLAIKQTLYRTSADSAVIDALERAARAGKYVS
                     VVVEIKARFDERRNIAWARRLEDAGAHVVYGLAGLKTHAKLLLVVRKEPGGIRRYLHL
                     ATGNYNESTARLYTDFSFFTVDRVLGDDVAAVCNMLTGFTWPRSGARLAVAPLDMRQK
                     LLALIAREEDNARRGGKGMIRAMMNSLSDRGIIEALERAASAGVRIDLIVRGICLLVP
                     DRNISVRSIVGRYLQHARVSQFHNGGDEEFYLSSADWMTRNLERRVEFLFPVLDAGLR
                     LFLKNTLDLFFRDTLNAWELQADGAYRRVPGREETDSFTEAGCLIASLENPAPDEVLQ
                     HFVPIRHMYTDQGEQA"
     gene            3076..3459
                     /locus_tag="PLD82_03895"
     CDS             3076..3459
                     /locus_tag="PLD82_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOG64560.1"
                     /translation="MKRHFIKITLFVLVVSVVFGSFLAFAQPAQTTAGQDQKESVQQT
                     GSKNQAPGKSATDNSILYLAAALAVGFSSIAAGWAVSTVGSAALGAISEKPELAGRAL
                     IFVGLAEGIAIYGLIIAIMLIGKAG"
     gene            3463..3780
                     /locus_tag="PLD82_03900"
     CDS             3463..3780
                     /locus_tag="PLD82_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOG64561.1"
                     /translation="MYRCLLIGDPDTAAAFALAGVVVRTPASQSEAGELFRDALADAE
                     TAVLVITENFAAALSGDINLHRQQGRAPLVIEIPENLSDSFGGRSLMESIRSAIGISI
                     QAD"
     gene            3805..4413
                     /locus_tag="PLD82_03905"
     CDS             3805..4413
                     /locus_tag="PLD82_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOG64562.1"
                     /translation="MDELDKGRRLSEEILAEADRKAARILGKAEREAGAILAEAKSQA
                     AGYREKMLAEARVRAENEKQRILRGTELEIRKDSLVRYRDYCDTLIPEAAELARRQGF
                     EMHPDWLADSLARGLAVMGEDAGSITFSQELDDVQVKALVVRLRFSGMIRRDPGLAPV
                     EAVIASGDGDLRYEILVEELAIEHDREMRQAALEIHKGEEDD"
     gene            4406..>5553
                     /locus_tag="PLD82_03910"
     CDS             4406..>5553
                     /locus_tag="PLD82_03910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOG64563.1"
                     /translation="MTEGRIISVNGPIVKAEGLVDVAMMDLVHVGHMGLTGEIIRFDK
                     GLATIQVYEDGTGMQPGEPVTGTGMPMSVALGPGLLGGIYDGIQRPLSVLEEHSGSFL
                     LRGEKIFRLDETRQWEIRPQVRQGARVRGGEVLFEVDETFLVRHRVMLPPGLEGELEY
                     LAPAGMRGIRDPVWRIRDARGVQHEGGLFHWWPARKGRPYKGKRPVDRPLLTGLRIID
                     TFFPISKGGVAAIPGGFGTGKTMTQHALAKWSDADVIVYIGCGERGNEMTDVLNEFPK
                     LRDPKSGKPLMERTILIANTSNMPVAAREISIFTGIAIAEYYRDMGYDVAIMADSTSR
                     WAEALRELSGRLEEMPAEEGFPAYLPSRLAEFYERAGAVETLAGSPGSV"
BASE COUNT         1031 a         1547 c         1807 g         1168 t
ORIGIN      
        1 tgctttccca gccggtccag tccatcaaat cctggtgcgg gatcgaacag atcgaccgct
       61 gtgtcgctgt cagcgaggac atctccgggc ttcttgacag ggtccgggac accactggcg
      121 tctcccatga tttgacggcg gacaagtttg gcgaactccg gaaattcgtt ctggcatgcc
      181 cgcaggagga gatggcctcg cgctgggggc tggacgggaa ccgcgccgtg accatccgga
      241 tcgggctctt ggtgatcggg atgcttttcg aacagtcggg ggccggccgg atcatcttcc
      301 cgcccatcca cctgacatcg gcggtcgtgg atgcccttgt ccggacggag ggcggatttc
      361 ccgatctcct ccgggatggt tttgaagggg agatcctcga aggcgcgcac gccattgggc
      421 ggaaattcaa gtacgatgaa gcgcatgccc gggagtgcga gcggattgcc gggctgctct
      481 acgaccaact ggcgcagagc ttccagccgc gggaacgact actggtccgg gtggccgcca
      541 ttcttcatga tattggaatg tatatcggat ccaagggcca tcaccggtac tcagccgagt
      601 tggtggccgc ctccgatatc ctgggactct cggcctgtga ggtccaggag gtttcgtgga
      661 cggtccgctt tcaccgaaaa ggactgccgg gcgggaccgc catcggctac aaggccatgg
      721 accggtctgc ccgtcttgcg gtcttcagaa tggcggccat tctccggctg gccgatgctc
      781 tcagcgagcc gggtcttccc cggatcgaag cgatctccat caggtttggc gagagcgcgg
      841 cgatcctgtc cgtcgggctc gagggaaagc gctatgagtt tctcgagatc atgcgtgttc
      901 ttctcaacaa caagaaacag tatttcgaac attattttgg aaccgatctg gttcttgaga
      961 gactgcggac ggtggacgga ccatgagcgg gcgcttcttc aatcgtgagt ggagctggct
     1021 ggccttcaac agcagggtcc tcgatgagtc gcgccggccg ggaacgcccc tcctcgagcg
     1081 tgtccgattc ctttccatct cgtcctccaa ccttgaggaa ttcttcatga tccgggtggc
     1141 cgccctcaag cgactgggcg cgatgcacgt tccggcgcgt tcgccggacg gactcgaacc
     1201 acgggagcag ctcgccggga tccgcgagcg cgttctcggg atggttcgtc tgcagtaccg
     1261 ggtgtttcat gaagagctgc tccccgggct ggcctcccac gggatccgca tcatgttgca
     1321 gaaggaggag atcgagccct ggcttgacga actcttcatc ctctaccgcg attccatccg
     1381 gccggttttg acacccatct cggtcggagg gacgcatccc tttcccaatc tggtcacggg
     1441 ccggctctat ctggccgttt cgctcggggt ggacgaggcg cggacggacc agatcgagaa
     1501 aagcaggctt tcgtttgtcg aaatccctgt ccgcgcccac ggccggtttc tccgtctgga
     1561 agagggatgc ttcgttcccc ttgaactggt tgtccggacc ttccttccag agttgttcgc
     1621 tggatacagt gttgaagggg catggctgat caaggtgacc cgcgatgccg acctggacat
     1681 cgagcaggat gccgcgggtg acctgttgac gctgatcgaa aacaaactga cacgcatgca
     1741 ccaccgttcc gtcgtccggc tggagtatga acagggcatg gatgagacca tgcgcctgct
     1801 gcttgcccgg caactgggtg tgggcgaggc cgaccaatac gggatcgagg ggctttttac
     1861 cctgcatgat ctggccgggt ttgtggagcg gattccccgg cccggactcg tctggccgga
     1921 ggcgcaggcc gtccttgtcc gcgattttgc cgtggatggg gcctttgcag ccatcgcgac
     1981 ccgggacaga ctcctcctcc atccttggca ttcctacaaa cccgtgatcg atttcatcaa
     2041 ccaggccgcg gacgatccgg cggtgttggc catcaagcag acgctctacc ggacaagcgc
     2101 cgattcggcg gtgatcgacg cgctcgaacg ggcggcccgg gccgggaagt acgtctcggt
     2161 cgtcgtcgag atcaaggccc gctttgacga gcgacggaat atcgcctggg ccaggcggct
     2221 cgaggatgcc ggtgcgcatg tcgtctacgg tctggccggt ctcaaaaccc atgccaagct
     2281 ccttctggtc gtcaggaagg agccgggcgg gatcagacgc tatctgcacc tcgcgacggg
     2341 gaactataac gagagcacag cccggctcta tacggatttc tcgttcttta ccgtcgatcg
     2401 tgttctcggg gacgatgtcg ctgcggtctg caatatgctc acagggttta cctggcccag
     2461 aagcggtgcc cgtctggcgg ttgctcccct cgatatgcgg caaaaactcc ttgccttgat
     2521 tgcccgggag gaggacaatg cacgcagggg gggaaagggc atgattcggg cgatgatgaa
     2581 ctccctctcc gaccggggga tcatcgaagc acttgaacgg gcggcctccg ccggtgtccg
     2641 gattgatctg attgtgcgcg ggatctgtct tcttgtgccg gaccggaata tctcggtccg
     2701 cagtattgtc ggacgctatc tgcagcatgc ccgcgtctcg caattccata acggagggga
     2761 tgaggagttc tatctctcga gtgccgactg gatgacccgc aatctcgaac ggagggtcga
     2821 gttcctcttt ccggttctcg atgccggttt gcgtttgttc ctgaaaaaca cactggatct
     2881 tttttttcgg gatacgctca atgcctggga attgcaggct gacggggcgt accgccgtgt
     2941 cccgggccgg gaggagacgg attcgtttac ggaagcaggt tgcctgattg cgtcgctcga
     3001 gaatccggcc cctgatgaag tgttgcaaca ttttgtgccg attaggcata tgtatacaga
     3061 tcaaggagaa caagcatgaa aaggcatttc atcaagatta ccctctttgt cctcgtggtt
     3121 tctgttgtct tcggatcgtt tctcgcgttt gcgcaacccg cccagacaac agcggggcag
     3181 gatcagaagg aatccgtcca gcagacaggg agcaagaatc aggcacccgg aaagtcggca
     3241 accgacaaca gcattctgta tctcgctgcg gccctcgctg tcgggttctc ttcgattgcg
     3301 gcgggctggg ctgtcagcac ggtcggttca gcggccctcg gtgcgatcag tgaaaagccg
     3361 gaactggcgg ggcgtgcact gatctttgtc ggtcttgccg agggcatcgc catctatggg
     3421 ttgatcatcg ccatcatgct gatcgggaag gccgggtaat ccgtgtaccg ctgcctgttg
     3481 atcggagatc ctgacacggc agcggccttt gctcttgccg gagtcgttgt ccgtactccg
     3541 gcatcacaga gcgaggccgg tgagcttttt cgggacgcgc ttgccgatgc cgaaaccgct
     3601 gttcttgtga ttaccgaaaa ttttgctgcc gccctctccg gggacatcaa tcttcacagg
     3661 caacagggcc gggctcccct ggtcatcgag attcccgaaa acctttcgga ttcattcggg
     3721 ggacgatccc tgatggagtc gatccgcagt gcgatcggga tcagcattca ggcggactga
     3781 cgcgcggaga aaggagaatc aatcatggat gaactggaca agggtcgccg cctgagcgag
     3841 gagatcttgg ccgaggcgga ccgcaaagcc gcccggattc tggggaaggc cgagcgtgag
     3901 gccggggcga ttctcgccga ggccaaaagt caggcggccg ggtaccgtga aaagatgctc
     3961 gcggaagccc gcgtccgggc agagaacgag aagcagcgga ttctgagggg cacggagctt
     4021 gaaatccgca aggacagtct tgtgcgatac cgggactatt gtgatacgct catccctgaa
     4081 gctgcagaat tggcccgtcg tcagggtttc gagatgcacc cggactggtt ggctgattcg
     4141 ctggcccgtg gattggcggt gatgggcgag gatgccggga gcatcacgtt ctctcaggaa
     4201 ctggatgacg tgcaggtcaa ggcactcgtc gtgcgtctcc gcttctcggg catgattcgc
     4261 cgggatcccg gactcgcacc ggttgaggca gtgatagctt ccggtgacgg ggacctgcgc
     4321 tacgagattc tcgtggaaga attggccatt gaacatgaca gggagatgcg tcaggccgcg
     4381 cttgaaattc acaagggaga agaggatgac tgagggacga atcatctcgg tcaatgggcc
     4441 gatcgtcaag gccgaggggc ttgtggatgt cgcgatgatg gatctggtcc atgtcggcca
     4501 catggggctg acgggcgaga tcatccgctt tgacaaaggt ctggccacga tccaggtcta
     4561 tgaggacgga accgggatgc aacccggtga accggtgacc gggacaggga tgccgatgtc
     4621 ggttgcgctc gggcccggcc ttttgggtgg catctatgac gggattcagc gccctctctc
     4681 ggtgctggaa gaacactccg ggtcgttttt gctccgtggg gagaagatct ttcggctgga
     4741 tgaaaccaga cagtgggaga ttcgtccaca ggttcgccag ggggcccggg tccggggcgg
     4801 agaggtgctg ttcgaggtgg acgagacatt ccttgtgcgg catcgggtca tgcttccgcc
     4861 cggactggag ggtgagctgg aatacctggc ccccgccggg atgcgtggaa tcagggatcc
     4921 tgtctggcgt atccgggatg cgcgcggtgt gcagcacgag ggcggacttt ttcactggtg
     4981 gccggcccgc aaggggcggc cctacaaggg aaaacggccc gttgaccgtc cgctcctgac
     5041 cgggctgcgg atcatcgaca ccttctttcc catatcaaag ggtggtgtcg cggccatccc
     5101 cggcggcttc gggacgggga aaacgatgac gcagcacgcg cttgccaaat ggagtgatgc
     5161 ggatgtgatt gtctacatcg ggtgtggcga acgcgggaac gagatgaccg atgtgctgaa
     5221 cgaatttcca aagctcaggg atccgaaatc cggcaagccc ttgatggaac gcaccatcct
     5281 gattgccaat acatcgaata tgcccgttgc ggcccgcgag atatccattt ttaccgggat
     5341 cgccatagcc gagtactacc gggatatggg ctatgatgtg gcgatcatgg cggattcgac
     5401 atcccgctgg gcggaggcct tgcgggaact ctcgggacgc cttgaagaaa tgccggcgga
     5461 ggaagggttt ccggcctatc ttccttcgag gttggcggaa ttttatgaac gggcgggcgc
     5521 tgtggaaaca ctggccggga gtcccggttc cgt
//
LOCUS       DAOSHE010000094         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13034_length_5531_cov_3.483930, whole genome shotgun sequence.
ACCESSION   DAOSHE010000094 DAOSHE010000000
VERSION     DAOSHE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13034_length_5531_cov_3.483930"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..1097)
                     /locus_tag="PLD82_03915"
     CDS             complement(348..1097)
                     /locus_tag="PLD82_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOG64564.1"
                     /translation="MENERFSRTQALLGESGMQRLHEARIAVFGLGGVGTSTAEYLSR
                     SGAGHLWLFDFDRITPSSCNRLLTAGTSTLGMEKAAAMRARILDINPRAEVSAEALFL
                     GPENAAEVLAAGKFDLVIDAIDSLNPKTSLLEAAIRGGHPVISSMGAAARTDPSRVVT
                     GTIWETRNCPLATKVRQGLRRRGITAAIPVVFSPESPAAPLPPESDNAADANLRYPRG
                     RVRNIQPSCVWIPAVFAAHLMRLALTVLGVK"
     gene            complement(1110..2741)
                     /locus_tag="PLD82_03920"
     CDS             complement(1110..2741)
                     /locus_tag="PLD82_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG64565.1"
                     /translation="MRVLLGYLSQWIAPGEDFMSFLPAGLPILAAVLEREGHEVMLAN
                     YSGMTPKQALRHVREFSPGAAGFSLFTHNRFETFELAGLIRRALPDCRIFVGGPHASP
                     LGDEILKREASIDWVIRGEGEGILPDLVRLVEVNPVRKDRKMIPGQAPAPTDAAMESL
                     LANQLPPRATLSAATRITDLDSLPQGCLYGGKTFGLDPRIQYSLIIGSRGCAWACAFC
                     ASPTLWGRRVSFRSAHSIVDEIRQARDRFGIQYFHIRDDNFTLNKERVLEFASLLQRE
                     GPAVRFNCQARVDSVDDEILEALAESGLEHIQFGVESGSPATLERFAKDVSPDAVIRA
                     CRSARKAGIPASVYLIGAIPGETREDIRATNELLSKAGPNDAVVSPLAIFPGTELCRQ
                     ATDDGDMNDSLWFRSREPAIFAAIGDHAAIARLEELSSLAGRITPPAPEIPPSLEGLS
                     GMRLLSFGEQSAALGMDEQAGRLFAAAMERAPENPWCLYRLAEWHLDQGNPAEATRIA
                     QALTALVPRWEDAASLLEAALAAANPKKKRNNRQN"
     gene            complement(2783..4216)
                     /locus_tag="PLD82_03925"
     CDS             complement(2783..4216)
                     /locus_tag="PLD82_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOG64566.1"
                     /translation="MTLQEALHPECIVELKSRQKNEAIGELISILHTRHPSVNKSLVL
                     ARIQEKEALFSSVIREGVAIPHVRIEMDEPVLIAFGRSERGVDFFGDNRQPVHLIVLL
                     LTRPDRPEAHLEILKDLALILDNDTTLASLITAPTIEEIRRSLVGRTSSRSPGSSKTR
                     AGRIDEQITDTVLKHSAEMAKTLGISTIFVNCDTVPSPERLKPLLGQRNAPNRIIPVF
                     KETTVPEDYRRLSPEFILQLPEMDLTKAGQIKLALFLALTKRWIKRNDVVLYLLPGSA
                     IRGLSNIDIIPIRERFTDIVFVQPERFKSVLEPSVLQQLIKIAFDLAINGREGHKVGT
                     LFVAGDHLSVLPHTQQLIVNPFRTSSEDERYNILDPAIEEMVKELAQIDGAFVVAHDG
                     RILNAGAYIGTGKRKAEITKGLGARHQAAANITAVTRAVAVTVSETNGVVTVFQNGRT
                     IFIIEPVAKKLRVRLGDDGETRPVRKS"
     gene            4361..5335
                     /locus_tag="PLD82_03930"
     CDS             4361..5335
                     /locus_tag="PLD82_03930"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG64567.1"
                     /translation="MRFSEKRPFSVMLPRGTVRGSVVVLHGLNMKPECMLELAAVCAE
                     AGMAVVLGSLRGHGEQSPIGAWATVTSEDWLADAAACRNEAIRLAAGNPVWLIGFSLG
                     AACGFAEYLQRIERERKPATSCRSRHKTEHPVQVSTTTSCRSAAGGPPCPHAACGLAE
                     YLQNIKKKPETDVPRPFRGYAGIILFSPAIRLRPWAHAVRLLFPWPRIRLSSLAGVRY
                     QVHDHTSIAAYRALFALRRLIRTELSRVRRGLETCPLPPLTIIYDARDILTAGRALGR
                     WLPGETVPAFHRVRAKNTWHHLITDRRALGDEAWQKLTSLIREMLDSG"
BASE COUNT         1049 a         1545 c         1839 g         1098 t
ORIGIN      
        1 attcgggcga gggatgccca ggatacctct gcaacaggtt caggatatgc agatgacagg
       61 ccctatggtg attgatcagg ttgcaggctt ccctccggcc gtgaaacgct ttgtccatcg
      121 tgctgcttct gtggcacgat ttttttgtca atactggact ggtcaggagg gtgcatgagc
      181 gccggcctgg agacagacag attgcaggaa agaaccgggc cgcattgttc caagagaaat
      241 tttttggtgg cattgtgcga acgcgaatga tccgcttcca atttctctgc tttgatggca
      301 tcaggaaacg gcaggggaaa agcatgtatc ctcgggagat ttttccctta cttcacgccg
      361 agtaccgtca atgcaagccg catcaggtgg gcggcaaaga cggccgggat ccagacacag
      421 gatggctgga tattccggac ccgcccccgc gggtagcgca ggttcgcatc cgccgcgtta
      481 tcgctctccg gagggagggg agccgcgggg gactcgggtg aaaagacaac cgggatggcg
      541 gccgtgatcc cgcgccggcg caacccctgc cggactttcg ttgccaacgg gcagttccgg
      601 gtttcccaga tggttccggt aacgacccgg gatggatcgg tccgggcagc ggcacccatg
      661 ctggagatga ccggatgccc gccccggatg gcggcttcga ggagactggt ttttggattg
      721 aggctgtcga tggcatcaat cacaagatcg aacttcccgg cggcaagaac ctcggcggcg
      781 ttttcagggc cgaggaagag cgcttcggcc gagacctcgg cccggggatt gatgtccagt
      841 atccgggcac gcatggcggc cgctttttcc attccgagtg tacttgtccc ggcggtcagc
      901 aagcggttgc aactcgatgg cgtgatccgg tcaaaatcga agagccagag atgtcccgct
      961 ccgcttctgg acagatactc ggcagtcgag gtcccgaccc cccccaagcc aaagacggca
     1021 atccgcgctt cgtgcaggcg ctgcatgcct gattctccca aaagggcctg tgtccgggag
     1081 aagcgttcgt tttccatcat gactccctgt cagttctggc ggttgttccg ctttttcttc
     1141 gggttggcgg cggccagggc ggcctcgagc aggctggctg cgtcttccca gcgcggcacg
     1201 agggcggtca gggcttgggc tatccgtgtg gcttcggcgg ggtttccctg atcgaggtgc
     1261 cattcggcca gcctgtagag acaccaggga ttctccgggg cgcgctccat cgcggcggcg
     1321 aacaaccgtc cggcctgttc gtccatcccc agggcggcag actgttcccc aaaagacaac
     1381 agacgcattc cggagagtcc ctcgagtgat ggcggaatct ccggggcggg cggcgtgatc
     1441 ctccctgcca gcgacgagag ttcctcgagc cgggcgatgg ctgcgtggtc accgatggcc
     1501 gcaaagatgg ccggctcccg tgagcggaac cagagggagt cgttcatatc gccgtcgtcg
     1561 gtggcctgac ggcagagttc ggtcccgggg aaaatggcca gaggggagac gacggcatca
     1621 ttcggcccgg ccttagagag gagctcgttc gtggcccgga tgtcctcgcg ggtctcgccc
     1681 gggatggccc cgatcagata gacggatgcc ggtatcccgg ctttacgggc cgagcggcag
     1741 gcccggatca cggcatcggg actgacgtcc tttgcaaacc gttcgagggt ggccggactg
     1801 ccggattcca ccccgaactg gatgtgctcc agtcccgact ccgccaaggc ctcgaggatt
     1861 tcatcatcga ccgagtccac cctggcctga cagttgaagc ggacggctgg accctcccgc
     1921 tgcaggaggg aggcgaactc gaggacgcgc tccttgttga gggtgaagtt gtcatcgcgg
     1981 atgtgaaaat actggattcc gaagcgatcc cgggcttgac ggatttcgtc aacaatggag
     2041 tgtgccgaac ggaaactgac ccgccgtccc cagagcgtgg gtgaggcgca aaaagcgcag
     2101 gcccaggcac agccccgtga gccgatgatc agcgagtact ggatccgggg atcgagaccg
     2161 aaggtctttc cgccgtacag gcatccctgg ggcaggctgt ccagatctgt gatgcgtgtg
     2221 gctgcggaca aggtcgcccg gggagggagt tgattcgcga gcaggctctc catggcggca
     2281 tcggtcggag ccggagcttg ccccgggatc atcttccggt ccttccggac cgggtttact
     2341 tcaaccagac ggaccagatc agggagaatg ccttcccctt cgccgcggat gacccaatcg
     2401 atcgaggctt cacgtttgag gatctcgtcc ccgaggggcg aggcgtgcgg tccgcccacg
     2461 aagatcctgc agtcgggcag ggcacgccgg atcagcccgg ccagttcgaa ggtttcgaaa
     2521 cggttgtggg taaagaggga gaaaccggcc gccccggggg agaactccct gacatgccgg
     2581 agtgcctgtt tgggagtcat gccggagtaa ttggcgagca tgacctcgtg gccctcgcgc
     2641 tcgaggacgg cggcgaggat cgggagaccg gccggcaaaa aggacatgaa gtcctcgccc
     2701 ggtgcgatcc attgcgagag atatcccaac aagacgcgca tctcagatat cccctttgtg
     2761 cagtgtcgta tccggcatgg tgtcagcttt tccggacggg tcgggtctcg ccatcatccc
     2821 cgagacggac acgcagcttc ttggcgacgg gctcgatgat gaagatcgtc cgcccgttct
     2881 ggaaaacggt gacaacacca ttggtttcgg agacggtgac agcgaccgcc cgggtgacag
     2941 ccgtgatgtt cgcggccgcc tggtgacggg caccgagtcc tttggtgatt tccgctttcc
     3001 gttttcccgt cccgatgtag gccccggcat tcaggatcct tccgtcgtgt gccacaacaa
     3061 aggctccgtc gatctgggcc agctccttga ccatctcttc aatggcagga tcgagaatgt
     3121 tgtagcgctc gtcctcgctg gatgtgcgga aggggttgac aatcaattgc tgggtatggg
     3181 gaaggacgga caagtggtca cccgcgacaa acagggtgcc gaccttgtgt ccctcacggc
     3241 cgttgatggc caggtcaaag gcgatcttga tcaattgctg aaggacggat ggttcgagga
     3301 ccgatttgaa acgctcgggc tggacaaaga cgatatccgt aaagcgttcc cggatgggga
     3361 tgatatcgat gtttgaaagc ccgcggatgg ccgagcccgg aaggaggtag aggacgacat
     3421 cattccgttt gatccagcgt ttggtgaggg ccaggaagag ggccagtttg atctgtccgg
     3481 ccttggtcag atccatttca gggagctgga ggatgaattc cggggagagc cggcggtagt
     3541 cttcggggac ggttgtctcc ttgaatacgg ggatgatccg gttgggggcg ttccgctgac
     3601 cgaggagggg tttgagtcgt tccggggacg ggacggtatc gcaattgaca aaaatggtgc
     3661 tgatcccgag ggttttggcc atttcggcac tgtgtttgag cacggtatcg gtgatctgct
     3721 catcgatccg gccggcccgg gtcttggagc tgcccggtga gcgtgaagat gtccgaccga
     3781 cgagcgaccg ccggatctct tcaatcgtcg gtgccgtgat cagggaggcg agagtcgtat
     3841 cgttgtcaag gatcagggcc agatccttga ggatctccag atgcgcctcg gggcgatcgg
     3901 gccgggtgag caacaggacg atcaggtgga ccggttgacg gttgtcaccg aagaaatcga
     3961 caccgcgctc gctccggccg aacgcgatca ggacgggctc atccatctcg atgcggacat
     4021 gggggatggc caccccttcc cggatgaccg agctgaagag ggcctctttt tcctggatcc
     4081 gggcgagcac cagggatttg ttgacggaag ggtgccgggt atggaggatg gagatcaatt
     4141 ccccaatggc ctcgtttttt tggcgtgact tgagttcgac gatgcattcc gggtgaagcg
     4201 cttcctgcag ggtcatatca gaacctcgtt ccgggtttat cgggaactct cccgacgaga
     4261 acagaacact cagtgaaacc gtgatcagga gcaccaggtg caggggctcg cagagagacc
     4321 cgcaccgttg taaaatacac agaaggagag gggactcata atgcgatttt cggaaaaaag
     4381 gccattttcc gtgatgcttc cccgggggac agtccgggga tctgtggttg tcctgcacgg
     4441 attgaacatg aagccggaat gcatgcttga actggctgcg gtctgtgcgg aggcggggat
     4501 ggccgtcgtg cttggttcgc tgcgcgggca tggggaacag tctccgatcg gtgcatgggc
     4561 gacggtgacg agtgaggatt ggctcgcgga tgcggccgcc tgccggaatg aggccatccg
     4621 tctcgcggcg ggcaacccgg tctggctgat cgggttttcc ctcggcgccg cttgcggttt
     4681 cgctgaatat ttgcaacgta ttgaaagaga acgcaagcca gcgacatcct gtcgtagccg
     4741 acacaagact gaacatcctg ttcaggtgtc taccacgaca tcctgtcgta gtgcggcagg
     4801 cggacctccc tgtccgcacg ccgcttgcgg tctcgctgaa tatttgcaga atataaagaa
     4861 aaaaccggaa acggatgtac cacgaccctt ccggggctat gccgggatca tcctcttcag
     4921 cccggccatc aggctccggc cctgggccca tgccgtccgg ctcctcttcc cctggccaag
     4981 gattcgcctg tcaagtctgg ccggggttcg ctatcaggtt catgatcata ccagtatcgc
     5041 ggcctatcgg gccctctttg cactgaggag gctgatccgg acggaactct cgcgcgtgcg
     5101 gcgcgggttg gagacgtgcc cccttccgcc gctgacgatc atctacgatg ccagggacat
     5161 cctgacagcg ggacgggcct tgggtcgctg gctgccgggg gagacagttc cggcattcca
     5221 cagggtacgg gcaaaaaaca cctggcatca tctgatcacc gaccggcggg ctctggggga
     5281 cgaggcctgg cagaaactga cgagcttgat acgggagatg ctggattcgg gttgaagagc
     5341 gggggcggct ttgggggact cttgtcaagg aatcctttgg ccagttcgga ggccggtcct
     5401 ctttgaccag gcacgggaat acccgatccg gacgggagtc ggcactcgct ttatggaaaa
     5461 aacggaaggc gtcacacgtt ccgggtggcc gggttgtgcg acacaaggct gggccggcaa
     5521 gcctcgccgg a
//
LOCUS       DAOSHE010000095         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13086_length_5509_cov_4.609094, whole genome shotgun sequence.
ACCESSION   DAOSHE010000095 DAOSHE010000000
VERSION     DAOSHE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13086_length_5509_cov_4.609094"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1177)
                     /locus_tag="PLD82_03935"
     CDS             complement(167..1177)
                     /locus_tag="PLD82_03935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG64568.1"
                     /translation="MDITIDFFRIEDYRKLGELTKRSFLLNAWHIDAHLPRPLGGEVF
                     FQTVATRALHENSNSCLVARFRQQPVGYIIYGVDAQIGRQLGVSAATIILFCVDESFQ
                     GKAIGERLLDRACILLGSRGVRLLTVGTDANNLPALTVYQRNGFVTRLNWATWRLYPG
                     FHRSPVAAPFRVEPWSGDEAVFDLCRLIDRPLSWFRDKRLSSSGLAEFRRSLAKLLAQ
                     HISDRRSMALVAKAGGFFRERIVGMLVWEEDGSVEKYYNTKEMDKRIYRVTDILVDRP
                     SRGQGVASQLLSAFCDMAASRSHCIEAWVAMDDWASINVVSKCSFRPVHMATVLHRWL
                     QA"
     gene            complement(1263..1799)
                     /locus_tag="PLD82_03940"
     CDS             complement(1263..1799)
                     /locus_tag="PLD82_03940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG64569.1"
                     /translation="MQGRLNNQLAENLERERNLDPFTRLKNRAAFLRDLGEEIERSNR
                     YKYRFSLILLDIDGLKMINEFYGRGAGDLAILTVRDVVNAAVRTVDSVYSLGSGSFAL
                     VLPNTDVSGGFILAERLRSTLESVVVPWEQDRELSLTVSCGLAVSGTNGLENADRVLD
                     RAELCLSRAKHGGRNRVC"
     gene            complement(2780..5476)
                     /gene="acnA"
                     /locus_tag="PLD82_03945"
     CDS             complement(2780..5476)
                     /gene="acnA"
                     /locus_tag="PLD82_03945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOG64570.1"
                     /translation="MEKRMLDQFKVRTQLGEKKDAPRIYSLKKLAETVGFRLEDMPYS
                     VRVLLESLVRNCDGKIIREDDVLAAARWRAESGEPLEVQFMPARVLLQDFTGVPAVVD
                     LAAMRDALAELGGDPAKITPLIPVDLVIDHSVQVDHSGSDDALEKNVTIEYGRNEERY
                     RFLRWGQQAFRNFRVVPPSSGIIHQVNLEHLATVVFEGEDGAGGRVLYPDSVVGTDSH
                     TTMINGLGVVGWGVGGIEAEAVMLGQPVSIVVGGVVGVRVSGKPKPGVTATDIVLTVT
                     DMLRKHKVVGKFVEFFGPGVPTLSLADRATLANMAPEYGATTGFFPVDAETLRYLELT
                     GRDAALTGQYYREAGLFCDENAPAPVFSSVVELDLSAIVPCVAGPKRPQDRVPLDEVA
                     DGFRKSLMAPVAERGFGVDKDKIGTRVALADGSGALGHGSVIIASITSCTNTSNPAVM
                     LAAGLVAKKAVDRGLRVPGYVKTSLAPGSRVVESYLARADLLQPLEKLGFHIVGYGCA
                     TCIGNSGPVSGAVADAVKSGDLVAAAVLSGNRNFEGRISPQARANYLASPPLVVAYAL
                     AGRVDIDFDTESLGSDHQGNRVWLRDIWPTDKELADAQAGSIDRGIFEQCYADVFYGD
                     SRWADISAGKGLRFDWDPASTYVRLPPYFEGLKKEAQAIQDITDARVLAVLGDSVTTD
                     HISPAGSIAKDGPAAKYLESQGVTRENFNSFGSRRGNHEVMMRGTLANIRLKNLMLPG
                     TEGGITKYVPSGETLSIYDAAMRYQKDGVPMVILAGRDYGMGSSRDWAAKGVQLLGVR
                     AVIAVSYERIHRSNLVGMGVLPLEFVDGQDAGSLGFDRSEVFSIPGLDRVTPGCRIEV
                     TAKKPDGRLVCFSTVVRVDAAIEVEYLKHGGILPMMVRHLRG"
BASE COUNT         1499 a         1681 c         1140 g         1189 t
ORIGIN      
        1 aaaaagcggc tttctctcgg gcggagaata ccggaatctg atcggcctgc accggttcaa
       61 ggtcgttgca agcgatttcc ggaaaaactc ttcatcaaaa accgtcgaga taacattcaa
      121 ataggtgcat cgtgacggca caggccactg aaccgacaca tccagcctat gcctgcaacc
      181 agcgatgcag gacagtggcc atatggaccg gcctgaacga gcattttgag acaacattga
      241 tcgacgccca atcatccatg gccacccatg cctcgatgca atgactcctg ctcgccgcca
      301 tgtcgcaaaa cgccgaaagc agctgagagg ccacaccctg cccccgagaa ggccgatcca
      361 ccaaaatgtc cgtcactctg taaatacgct tgtccatttc cttggtgttg taatattttt
      421 cgacagaccc gtcttcctcc caaaccaaca tcccgacaat gcgttccctg aaaaaccctc
      481 ccgcttttgc gaccaaggcc atgctccgtc tgtcagaaat atgctgcgcc agcaattttg
      541 ccaaactacg ccggaactct gccagaccac tggaagacag ccgtttgtcc cgaaaccagg
      601 agagtggtct gtcaatcagt ctgcagagat caaatacggc ctcatccccg gaccagggct
      661 caacacgaaa aggcgcagca accggtgatc gatgaaaacc gggatagagt cgccatgtcg
      721 cccaattcag ccgggttaca aaaccattcc gctgatagac tgtcaaagca ggaagattat
      781 tggcatctgt tcccaccgtc aagagacgga cccccctgct tccgaggaga atgcaggccc
      841 gatccagcaa tctttcacct atcgccttgc cctgaaacga ttcatcaacg caaaaaagaa
      901 tgatcgttgc cgcagaaacg cccaactggc gaccgatctg cgcatcaact ccataaatga
      961 tatacccgac cggctgttga cgaaaacgcg ccaccagaca actgttgctg ttttcatgca
     1021 aggctcttgt cgctacggtt tgaaaaaaga cctcccctcc gagaggccgg ggaagatgtg
     1081 catcgatgtg ccaggcgttc aataaaaacg agcgttttgt aagttcaccc agcttccggt
     1141 aatcctcaat acgaaaaaaa tctatggtaa tatccatgac acaaaccttt gcattggtca
     1201 cactctgccc ggagtcaaat cgccggcatt ttcacataat ttgacagttc cgcccttcag
     1261 cgtcagcaaa cacgatttct tccaccgtgc ttcgctctgg acaggcacaa ctcggcccga
     1321 tccagaaccc tgtccgcatt ttccaaacca ttcgttcccg agactgcaag gccacaggat
     1381 accgtcaaag aaagctctcg atcctgttcc cacggaacca caacgctttc gagcgtggac
     1441 ctcaatctct cggcaaggat gaagcccccg gagacatccg tattcggcaa aacaagcgca
     1501 aaagaaccgc ttccgagaga atacacagaa tcaaccgtcc gcacagctgc attcaccaca
     1561 tcccgcactg tcagaatcgc cagatcgccc gcgcccctgc cgtaaaactc gttaatcatt
     1621 ttcaatccgt cgatatccag cagtatcaat gaaaacctgt acttgtacct gttggatcgt
     1681 tcaatctcct caccaagatc acgaagaaaa gccgcacggt ttttcaatct tgtgaacgga
     1741 tcgagattcc gctcacgctc aaggttctct gccagctggt tgttcaatcg cccctgcaca
     1801 gaaactgtcc gcaagccaaa aaaagttacc aaggcaaaaa gcaatccgat gataaccaat
     1861 gagacaaccc ggccctcttg gcccggaatg tccgccaaaa ccagcgaaag gaacaaaagg
     1921 ctgctgacca agcatgatac cgaaaatgcg gtatagaatg tgcgagtatt tttcatacag
     1981 gcgcattcta tacagagccg attcaagagt caagaaaaag catgttcgtg tttcaacaca
     2041 gacgatattg aagaccagct tcaccccaac tgctcgttat cacgaccaca agcgataacg
     2101 aaacccgtcc ctcaaacgaa cattgtcacc atgatgattt tttttataaa atacatggca
     2161 ccgcaagaca ctcccatgcc tctcctctat aatgaataac ccggaggtta tatgaatttc
     2221 actctttctg acaacaaacg ttgttcaata tacggtgttt ttcgttttcg ttgtcgaatg
     2281 caaaagcggt cctatatcta tgcatcatcc cagacagacc aaatctccaa aacatgccga
     2341 aaaattttgc tgcactttgc atcggctatc aaaaacggta ttctcaactt gcaagccaga
     2401 agccagttcc gatcacgtcc aaatcatgca gacaagcgag aatgtgtcct tgttctgtca
     2461 aaagacatga aagactggat tgaaaaaata tcatttgcag aaagaataag ccaatccgag
     2521 ataatcagaa tggctctcga atggtggatc gaaatctcat cggacaaacc tcgaagcact
     2581 gccctgaaaa aatggcagaa tgaccacata tctcttcgaa tcatggaatt ctacttctca
     2641 ttctggaaat acgcacgaca tctgacaagc acatttccag aaaccggcag atcatccgaa
     2701 tgtttgccag agccgtgcca ataataacaa aacgcatctc catacgcaat tcgagacctt
     2761 cttgaaaaga ctctcaccac taccctcgaa gatgacgaac catcattggc aggatgccac
     2821 catgtttgag atactcaacc tcgatagcag catctacccg gactactgtc gagaaacaga
     2881 ccaggcgacc atcgggtttc ttggcggtaa cctcaatgcg acacccggga gtaacacgat
     2941 caagccccgg tatcgaaaac acctcactgc gatcaaaacc gagggacccg gcatcctggc
     3001 catccacaaa ttccagcggc aaaacgccca tcccgacaag attgcttctg tgaatacgct
     3061 cataactgac tgcaataaca gcccgaaccc ccaaaagctg caccccttta gccgcccaat
     3121 cacgtgaaga gcccatcccg taatccctgc cggccaatat caccataggc accccatctt
     3181 tttggtatct catggctgca tcatatattg acagggtctc tccggatggc acatatttcg
     3241 tgatgccacc ttccgtcccg ggcaacatca agttcttcaa gcgaatattt gccaaggttc
     3301 cccgcatcat gacctcatga ttccctctcc tggaaccaaa agaattgaag ttctcacgag
     3361 ttacaccctg actctccaga tattttgcag ccggaccatc cttcgcaatt gaacctgcag
     3421 gtgaaatatg gtcggttgtc acagaatcgc ccaaaactgc caagacccgg gcatcagtaa
     3481 tgtcttgaat agcttgcgct tcctttttca atccttcaaa atacggagga agacgaacat
     3541 acgtcgaagc cggatcccaa tcgaacctca gcccttttcc tgcggatata tcagcccacc
     3601 ggctgtcacc ataaaacaca tcagcatagc actgctcgaa tatcccccga tctattgacc
     3661 cagcttgagc atctgccaac tccttgtcgg ttggccaaat atctcgcaac caaacgcgat
     3721 ttccctgatg atcgcttccc aggctttctg tatcaaagtc aatatccact ctgcctgcca
     3781 aggcataggc aacgacaagc ggtggggatg caaggtaatt tgccctcgcc tgggggctga
     3841 tcctgccttc aaaatttctg tttccggaca ataccgctgc cgcaacaaga tctccgctct
     3901 taacggcatc agccacagca ccgctcaccg ggccactgtt tccgatacac gtcgcacacc
     3961 cgtatccgac aatatgaaat ccgagtttct cgagcggttg caacaaatca gctcgcgcca
     4021 gatagctttc aacaacccgc gagccaggcg ccagactcgt cttgacatac ccggggacgc
     4081 gcaaccccct gtcaacagcc ttcttggcaa ccagtcctgc agccagcata accgccggat
     4141 tggaagtgtt tgtacagctc gttatcgagg cgatgatgac actgccgtgc cccaacgcac
     4201 ctgagccgtc ggcaagtgct acccgtgtac caatcttgtc tttatccacc ccgaaacctc
     4261 gctctgcaac cggtgccatg agcgatttcc tgaacccatc ggcaacttca tcaagcggaa
     4321 cccggtcctg cggtctcttt ggccccgcca cacagggtac aatggccgaa agatccagct
     4381 cgaccacgct tgaaaatacc ggcgcaggag cgttttcatc acaaaagagc ccggcttcac
     4441 ggtaatactg tcctgtcaag gcagcatctc ttcccgtcaa ttccagatac cggagagtct
     4501 cggcatcaac cgggaaaaag cccgtggttg caccatactc gggtgccata ttggccaggg
     4561 ttgcccgatc cgccaaggaa agggtcggaa caccgggccc gaaaaactcg acaaatttcc
     4621 cgacaacttt gtgcttgcgc aacatatcgg ttacagtcaa cacaatatct gttgctgtca
     4681 ctcccggctt gggctttcca ctgacccgaa ctcccacaac tccacccacg acgatgctca
     4741 cgggttgccc aagcatgaca gcttcagcct caatacctcc gacaccccaa ccaacaaccc
     4801 ccagtccatt gatcattgtt gtatgagaat ccgtccccac gaccgaatcc gggtacagaa
     4861 cccggccacc tgcgccatct tcaccttcaa agacaacggt agccagatgc tcaaggttca
     4921 cttgatgaat aatccctgac gacggtggaa caaccctgaa attcctgaaa gcctgctgtc
     4981 cccagcgcag aaaccggtat ctttcctcgt ttcgcccgta ttctatggtg acattttttt
     5041 cgagcgcatc gtcacttccg gaatgatcga cctgaacgga atggtcaatg accaaatcca
     5101 cggggatcaa gggggtgatc ttcgccgggt ctccaccaag ttcagccaga gcatcccgca
     5161 tcgcagccag atccacaacc gcaggaaccc cggtaaagtc ctgcaaaagc accctggccg
     5221 gcatgaactg gacctcgagt ggctcgccgc tctcggccct ccagcgagcc gccgcgagaa
     5281 catcatcctc cctgataatc ttgccgtcac aattcctgac cagcgactca agaagaaccc
     5341 tcaccgaata cggcatatcc tcaagcctga aaccgactgt ctctgccaat ttcttcaaac
     5401 tgtatattct cggagcgtct ttcttctcgc caagctgcgt tctcaccttg aattggtcca
     5461 gcattctttt ctccatatcc gggggattct tccacttcaa gatacaaaa
//
LOCUS       DAOSHE010000096         5419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13362_length_5419_cov_4.128262, whole genome shotgun sequence.
ACCESSION   DAOSHE010000096 DAOSHE010000000
VERSION     DAOSHE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13362_length_5419_cov_4.128262"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="PLD82_03950"
     CDS             complement(<1..2820)
                     /locus_tag="PLD82_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64571.1"
                     /translation="MLHVQLFYNVDLIFGSIFSLLALFLYGLPAGLFVSITSSAWTWG
                     HPWAMLILIGETVFCGWAFSKKRLNPIHAVLAYWLVLGMPQVLLWYHLALGMEIEPAI
                     LVMLKQCFNAMFSALVASTCIIGLEFFSRTRGRGHYHKTLDLAFIIQVFLLGFLILPS
                     LTLIIIQTRDRVHTIETTITVRLEDSILSLEQFVLSRTGLTQQRLESIARKSLLLGED
                     FARNELTQLAGESAPGELIAGISDAHGTTLWSSDNQFREVPTNACATISCSNHAAPAM
                     ILVQIAANQRRFWATLSTGELVAWLSGQLTRSRIRQAILFRTGHHIEGATNAPTPLAF
                     FAHDVFQHQEIGPNLVMAIPKGKFPSAMMRWGKSCIYTTRPFPGNPGLTLVAEAPLAP
                     AISTIADSIKLVLIILVACVILSTPVAWLIGRRTTRSLIRLAEISKNIPGRLAAGGPE
                     PGWHTSAITEIQALTSNFREMALAISGQIRDLRQTSEAKSRFLANTSHEIRTPLGGII
                     GISRQLLRGRLDQKQREQVLMMRSSAESLLGIINSILDFSRIESGRMEVDPHHFTLSG
                     IIDPLAQEYTMACEEKGLRFRLDARTPLAVVVYGDSGKLRQILVNLLGNALRFTEAGQ
                     ITLGIGRVDSHYRFTVEDTGPGISPEQRQRIFESFMQADVTTTRKHGGTGLGLSIARD
                     LAALMGGLINLDSTPYKGSTFVLQIPLEDGNPKLVEVADQHAVDDTSGLSGMKILVAE
                     DNAINRKYLAALFETLGCSASFAVNGSEAVHKSGVGEYDCILMDIQMPVMDGIEATTL
                     IRQRESRMGEHIPIIALTASAFESDRERFLSCGMDDYLSKPVDENTLIRILLSLRKDV
                     PPETPASTPALDLSGLIQAYNALGNETLSEVLDIFAREYPGRRERIQKAFTDHDTEMI
                     RFEIHSLKGISSNLYVRHYFCPFIRE"
     gene            3046..3747
                     /locus_tag="PLD82_03955"
     CDS             3046..3747
                     /locus_tag="PLD82_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG64572.1"
                     /translation="MAERSVLIIEDEHKIRDVLRAYLVREGLVVFEAADGTSGLEACE
                     QHRPDLVLLDLMLPGMSGERVAAAIRQKGEIPVIMLTARSEEWERLQGFELGADDYIT
                     KPFSPREVVARVRAVLKRSGHAMTQDEPLCIIGGISIEPASRMVSVSGQALSLTATEF
                     DLLHEMARHPGKVYRRSQLAALVLGYDHESMERTIDSHIKNLRRKLGNEARCIETVHG
                     VGYRCSQGENGGERS"
     gene            3744..5102
                     /locus_tag="PLD82_03960"
     CDS             3744..5102
                     /locus_tag="PLD82_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64573.1"
                     /translation="MSQRLRTRLMLVYVLAALGVFGLAAVGIHLAVMDRFERFAAQNR
                     EQRRGDFIRMISTLVSEKNVINQRGLRMAMRRFGVRGVTIRDTTNATLFEMRLDDQSG
                     PGRRHPLAMPDGALQVWREDLIVDGRVAGRIEVQAIGDPPHFAMIEKVFTEEVLRTLV
                     IATGVATLIFGFLSWSFARTIARPLEAASEAAGRIAGGDLAARLTPGGVREVAELADD
                     FNRMADSLARKESVRTRMTSDIAHELRTPVSVLKSHLEGIRDGILPADEDEVLSLLDE
                     TGRLERIINDLRAIWELENAGTRLRMETLVLDPALARLADRFRTLARTRGITIGFEGA
                     AGCRVLADESAFERALSNILSNAIKYGRDHGQVLLTCVRESATIRIGVRDDGPGIPEA
                     DLPHVFERFYRADEARVRSDGGAGLGLAIAAEAIAACQGSIWAENNPEGGCTFFVRLP
                     LA"
BASE COUNT         1100 a         1419 c         1763 g         1137 t
ORIGIN      
        1 ttctcggata aatgggcaaa agtagtgccg tacatagaga ttgctgctaa ttcctttcag
       61 gctgtggatc tcgaatcgga tcatctccgt gtcatggtcc gtaaatgcct tttggatgcg
      121 ttccctcctg ccggggtatt ccctggcaaa gatatcgagg acctcggaca gtgtctcgtt
      181 tccgagggcg ttgtacgcct ggatcaggcc ggagagatcc agtgctggtg tgctggccgg
      241 tgtttccggg ggaacgtctt tcctgagcga gagaaggatg cggatcagcg tattttcgtc
      301 cacgggtttg ctcaggtagt catccatgcc gcaggagaga aaccgttcgc ggtcgctctc
      361 gaaggcggac gccgtcaggg caatgatcgg gatatgttcg cccatcctgc tttcacgctg
      421 cctgatgagg gttgtggcct cgatcccgtc catgaccggc atctggatat ccatcaaaat
      481 acaatcgtat tccccgaccc ccgacttgtg gacggcttcc gagccattga cggcaaagct
      541 ggccgaacac ccgagtgtct cgaacagggc ggcgagatat ttccggttga tggcgttgtc
      601 ttcggcgacg aggattttca tcccgctcaa tcccgaagtg tcatcaacgg catgctgatc
      661 ggccacttca accagttttg ggtttccgtc ctcgagaggg atctgcagga caaatgtgct
      721 ccctttgtac ggagtgctgt ccagattgat caaacctccc atcaacgcgg ccagatcccg
      781 tgcgatggaa aggccgagac cggtacctcc atgcttgcgg gttgtggtca catcggcctg
      841 catgaagctc tcgaagatcc gttggcgctg ttccggagag attccgggac cggtatcctc
      901 cacagtgaat ctgtaatggc tgtcaacccg gccgatcccc agggtgatct gcccggcctc
      961 ggtaaagcgc agggcgttcc cgaggaggtt gaccagaatc tgtctgagtt tgcccgaatc
     1021 tccgtagacc acaacggcaa gtggcgttcg ggcatcgagc ctgaaacgca gacctttttc
     1081 ctcgcaggcc atcgtgtact cctgagccag gggatcaatg atcccggaca gggtgaagtg
     1141 gtgcggatca acctccatgc ggccggattc gatccgcgaa aaatccagaa tcgagttgat
     1201 gatccccagc agggattccg ccgaggagcg catcatcaag acttgctcac gctgtttttg
     1261 atccagcctg cccctgagga gttgccggga gattccgatg atcccgccca ggggtgtgcg
     1321 gatctcgtgg ctcgtgttcg ccaaaaagcg ggacttggcc tcgctggttt gtcgcagatc
     1381 acggatctga cccgagatgg ccagggccat ctcccggaaa ttcgaagtga gtgcctggat
     1441 ttcagtgatg gcacttgtgt gccagccggg ctcaggaccc ccggcggcca gccgcccggg
     1501 gatgtttttg ctgatctcgg ccagccgtat caatgagcgg gtcgtccgcc gtccgatcag
     1561 ccaggcgacc ggggtcgaga ggatgacgca ggcgacgagg atgatcagga ccagctttat
     1621 tgaatcagca atggtggaga tggccggagc cagaggcgct tcggccacca gggtcagtcc
     1681 gggattcccg ggaaaggggc gggttgtgta gatgcaggac ttgccccagc gcatcatggc
     1741 ggaaggaaat tttcccttgg ggatggccat gacgagattg ggcccgatct cctggtgctg
     1801 gaaaacgtca tgagcgaaaa aggccagggg agtgggtgca tttgtggccc cctcgatatg
     1861 gtggcccgtt ctgaagagga tggcctggcg gatgcgggag cgtgtcaatt ggcctgaaag
     1921 ccaggcaaca agttctcctg tggacagggt ggcccagaag cggcgctgat ttgccgcaat
     1981 ctggaccagg atcatggcgg gtgcggcatg attggagcag gagatggttg cgcaggcgtt
     2041 tgtcgggacc tcgcggaatt ggttgtcgct ggaccagagt gttgttccgt gagcatcgct
     2101 gatcccggcg atcaactcac cgggggcgct ttcaccggca agctgggtca gctcgttccg
     2161 ggcaaagtct tcacccagga ggaggctttt gcgggcgatg ctctccagcc gctgttgggt
     2221 cagtccggtg cgcgagagga cgaattgctc caggctgagg atcgaatctt cgaggcggac
     2281 cgtgatggtt gtttcgatgg tgtgaacccg gtcacgggtc tggatgatga tcagggtcag
     2341 ggagggcagg atcagaaaac cgagcaaaaa gacctggata atgaaggcca gatcgagggt
     2401 tttgtgataa tgccctcgtc cacgggtccg ggaaaagaac tcgaggccga tgatgcaggt
     2461 gctggcgacc agggccgaga acatcgcatt gaaacactgt ttgagcatga cgagaatcgc
     2521 cggctcgatc tccatgccga gtgcaagatg gtaccagagg agaacctggg gcatgccgag
     2581 gaccagccag taggccagga ccgcgtggat cggattgagg cgttttttgg aaaacgccca
     2641 tccgcaaaaa acggtttcgc cgatcaggat cagcatcgcc catgggtggc cccaggtcca
     2701 ggccgatgag gtgatactga caaagaggcc ggcggggaga ccgtagagaa agagggccag
     2761 gagggagaag atgctcccga aaatcagatc cacgttgtaa aaaagctgga cgtgaagcat
     2821 gtttcccaaa aaaccaagga tcgcgagcag cagaatgatg ccggtctttc gttgtctcgg
     2881 gcaggttgta tcggatatca tgctctcagt gtatgacaag ccggttgtgt ctgcaatcag
     2941 tgtttctacg gatgtccaca cgggatcttc gagtggcctg catacaatct tcacaatcag
     3001 gcagtactat ttttgatgaa cattctcagt ccgggaggac gcggcatggc cgagcgaagt
     3061 gtgctgatta tcgaggacga acacaagatc cgggatgtgt tgcgggccta tctcgtcagg
     3121 gaggggctcg tcgtgtttga ggccgccgat ggaaccagcg gtcttgaagc ctgcgagcag
     3181 catcgtcctg acctggtcct gctggacctg atgcttcccg ggatgtccgg tgagcgggtg
     3241 gcggccgcca tccggcagaa gggggagatc cccgtcatca tgttgaccgc ccgcagtgag
     3301 gaatgggaaa gactgcaagg atttgaactt ggtgcggatg attacatcac caagccgttc
     3361 tcgccgagag aagtcgtggc ccgggtgcgg gctgtcctca aacggagcgg tcatgccatg
     3421 acgcaggacg agcctctttg tatcatcgga gggatcagta tcgaaccggc ttcgcggatg
     3481 gtcagtgttt ccgggcaggc cctgagcctg acggcgaccg agtttgacct cctccacgag
     3541 atggcgcgtc atcccggcaa ggtataccgc cggtcccagc ttgctgcgct cgtgctggga
     3601 tatgaccatg agagcatgga acggaccatc gacagccata tcaagaatct gcggcggaag
     3661 ctgggaaacg aggcccgttg catcgagacc gtgcatgggg tcggctaccg ttgctcacaa
     3721 ggcgagaacg gcggtgaacg ctcatgagtc agcgcttgcg gacccggttg atgctggtct
     3781 atgttctggc tgcactgggc gtattcggac tggcggcggt cggcatccac ctggccgtaa
     3841 tggatagatt cgagaggttt gccgcacaga acagggaaca gcgtcggggg gatttcatcc
     3901 ggatgatctc gacgctcgtt tcagaaaaga acgtcatcaa tcaacgtgga ctgaggatgg
     3961 ccatgcggcg gttcggagtc cggggtgtca ctatccggga tactacaaac gcgacactct
     4021 tcgaaatgcg gctggatgac cagtcgggtc ccggtcgccg gcatccgctg gcgatgcctg
     4081 atggcgcgct ccaggtttgg cgggaagacc tcattgtcga cggacgcgtt gccggccgga
     4141 tcgaggtcca ggctatcggg gatccgccgc attttgccat gatcgagaag gtttttacgg
     4201 aggaagtgtt gcggaccctg gtgatagcga cgggtgtggc cacgctgatc ttcggattcc
     4261 tgtcatggtc ctttgccagg acgattgccc gaccgctgga agccgcgtcc gaggcggccg
     4321 ggcggatagc cgggggtgac ctcgcagcca gattgacacc cggcggggtg cgggaggtcg
     4381 cggaactggc cgatgacttc aaccggatgg ccgattcgct ggctagaaag gaaagtgtgc
     4441 gcacgaggat gacctcggat attgcgcacg agctccggac tccggtctcg gttctcaagt
     4501 cccatctcga ggggatccgg gacgggatcc tgccggctga cgaggatgag gtgctttccc
     4561 tgctggacga gacggggcgg ttggagcgga tcatcaatga tcttcgggct atctgggaac
     4621 tcgagaatgc cggcacccgg ctcaggatgg agactcttgt gcttgatccg gctctggcca
     4681 gactggccga ccgtttcagg actctggcca gaacccgggg tatcacgatc ggtttcgagg
     4741 gggccgccgg gtgccgggtg ctggccgatg aatcagcctt cgaacgcgcc ctttcgaaca
     4801 tcctctccaa cgccattaag tatggaaggg atcatggcca ggttttgctc acctgtgtcc
     4861 gggagtcggc gacgatccgg attggagtca gggatgacgg gcccggaatt cccgaggccg
     4921 atcttccaca cgtctttgaa cggttttaca gggcggacga agcccgggtg cgcagcgacg
     4981 gtggcgctgg tctggggctg gcgattgccg ccgaagcgat cgccgcctgt cagggttcga
     5041 tttgggccga gaacaatccg gagggtgggt gcaccttctt tgtccgcctc ccgttggcct
     5101 gagcaagccg gctttgcctt ccggaaggcg gttgaagagc atccacatga tatgggaata
     5161 tgactattgt ataaaaaaat ccgataaatg ttgacaggaa tttgcttccg gtcaagtatg
     5221 gtaccaaggg gtccgtccct cgcaaaagag cggccccaca ctacttaaaa aggacggaat
     5281 tcaaaatgaa gagaattatc aggctagtga gtgttgtatc catgcttttg gtcgggctgg
     5341 ccgcctgcag tgttgctccg gatgttgccg gtgtggctga tgacactgct tcccgcgctg
     5401 taacgaccta caccgaaac
//
LOCUS       DAOSHE010000097         5404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13423_length_5404_cov_4.178538, whole genome shotgun sequence.
ACCESSION   DAOSHE010000097 DAOSHE010000000
VERSION     DAOSHE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13423_length_5404_cov_4.178538"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLD82_03965"
     CDS             complement(<1..427)
                     /locus_tag="PLD82_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG64574.1"
                     /translation="MESNPRRTRIVATLGPASRNRETLAAILEAGVNVIRLNLSHGSH
                     EEHRENITFVRETAALLGLSVGILADLQGPKIRTGQIAGGEIQLETGDTIVFTPDQAD
                     GSDGRVSISYASLCEDIKPGNRILIDDGLLDAEVVEVRGR"
     gene            complement(809..>1818)
                     /locus_tag="PLD82_03970"
     CDS             complement(809..>1818)
                     /locus_tag="PLD82_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG64575.1"
                     /translation="VMTRESYLEYSRIGMGRALARAQREAVWSLHEKLVEQALAEGKG
                     VPSLAWWLLLGHLTQAGQSGARWARVFVDECQDLTGTKLAILRRQAEHLVLAGDSEQM
                     IYRGTSPFLDAGMDMRRVTTVSLSQVYRNTIPIFEFARRVREMIPDAEEGGTGHATRM
                     GPVPDVHFESGWNGLLDHLVERIRMLAFRDDALYEPGSIAVLTLGRPEDYAARLEARN
                     IKVRIAKHDDVDFAKTDAVFVSTIHTAKGMDFPVVFVLLDEIEEQRRHYLDDGADPAA
                     CAPRLMRLLYVASTRGMDILEIFCQQSGGREVPEVRALKAAHQEMLALFEGDGETRHP
                     E"
     assembly_gap    1819..1918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1919..3165)
                     /locus_tag="PLD82_03975"
     CDS             complement(<1919..3165)
                     /locus_tag="PLD82_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG64576.1"
                     /translation="MDHDKDATRLEKLYHADPGFFCIATHAWMEAWCQEVMPRVRSRF
                     TRPDGICTISFYDYLDELRRVIGSDPAMSRRWAGSMELLATVKYMHQETNTVRHAFAT
                     IRRERADDYLCTLVTFMQNAGVDPAIRDRLVALRQTGPLDYQNLLRDLVELRAENREI
                     RESLAVAEEKGLASLQTRLDELQADVRDKEREIQRLHDEGARLDSKAGRVDQLRAEMT
                     DHKKKILELQNLIDRVSKREAFYRSMACHSRTRVLLERNLMQLGFDQEEAVRQFHDQP
                     VFLVKGPAGSGKTIVLLKCVARALELAGQGELLEGPDSRPLVMNWGKRVQAYSEYQIK
                     VHEMFEGDQVEMKTVDSWLAGLVKKHVRLDVDWTARLADLVARMGAIEGLAGVEAELE
                     MLWTRGVMTRESYLEYSRIGMGRA"
     gene            complement(3304..3660)
                     /locus_tag="PLD82_03980"
     CDS             complement(3304..3660)
                     /locus_tag="PLD82_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG64577.1"
                     /translation="MTKAEIVESISREVDVQKKDIAFVIDSFFEKVKGSLSEGDPMEL
                     RGFGTFGYKIRKGRKARNPRSGEAVVVDDRVVIFFKPGKEFKQLAKLVPVEKIRSMVE
                     SRKRGPRGSKAPDDEA"
     gene            complement(3706..3978)
                     /gene="rpsT"
                     /locus_tag="PLD82_03985"
     CDS             complement(3706..3978)
                     /gene="rpsT"
                     /locus_tag="PLD82_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG64578.1"
                     /translation="MSKRYHSGLKANRQSITRKMRNKSEMSKIRTQVKKSRVALTEGK
                     KDEAVALMPELFSVLDKGVKHGRLHKNTVARKKARMAKKLASLVKA"
     gene            4238..>5404
                     /gene="uvrC"
                     /locus_tag="PLD82_03990"
     CDS             4238..>5404
                     /gene="uvrC"
                     /locus_tag="PLD82_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG64579.1"
                     /translation="MNETLPLDERVRTLPDLPGVYLMKNSDGTIIYIGKARRLKRRVA
                     SYFNKNHDSAKTQALVRQIASIDYIVAGSESEALLIEANLIKAHTPRYNIQLRDSKRY
                     PWLAVSIGEDFPRFFKTRNPGDTGLRTFGPWPNVSVIRNTISILHRLFGFRYCRKRLV
                     EGTPEPPVCFYYHIKRCPGPCEGHISRADYRAGIERAILFLEGRHDDLRVMLEEQMRA
                     HARALEFEKAAAIRDQLAAIAEAGTVQAVHRVIEEGAPADLDIAAMAEHSGMAAFAVM
                     EIRGGKLLAQHLFLENDADSKPELFFQFLRAWLQTQSSGDDPRPLLLIPDSSIEDEHI
                     EEGSVMKRRLLPAGEADPGLRRLLAQARSNAALALEEQISRRLYADGLTDLQKIL"
BASE COUNT         1085 a         1755 c         1385 g         1079 t
ORIGIN      
        1 cacgcccgcg gacttcaacc acctcggcat caagcagccc gtcatcgatc aggatcctgt
       61 tgccgggttt gatgtcttcg cagagactgg catagctgat cgagaccctc ccgtccgagc
      121 catccgcctg gtcaggagta aagacaatgg tgtccccagt ctcaagctgg atctcgcccc
      181 cggctatctg tcctgtgcgg atcttgggcc cctgcaggtc ggccaggata ccgaccgaga
      241 gccccaggag ggcggccgtc tcacggacaa aggtaatgtt ttcgcggtgt tcctcgtgcg
      301 aaccgtggga gagattgagg cggatgacat tgacaccggc ctcgagaatg gccgccagcg
      361 tctcacggtt gcgggatgcc gggccaagtg tggcgacgat gcgggtacga cggggattgg
      421 attccatggt tcgcgaaaca ctccttgcaa cagatctgcc gtaaagatac gcatgaatcc
      481 gccgcggcac aaacaacggc cgggacaccg gtatggctgt gcacacactc gacgcagcac
      541 aatcgttgca caatccgcca cgatccggac aaatcaatgg aagcgttttt gatggaaaat
      601 atttttggtg aacaacctca ggccaatcca cgcgatgtct tggcgcgacg aatgctgaat
      661 ttgtgatggc actccgggag tcgcatgatt gtattgtgaa tttgtgtttc gaagacgcga
      721 tcaatcgcgt ctctacgaaa gaccatgcca accgccaaat cacgcgaatc aacacgaatg
      781 gctcatgggc gctggcttgg ttcatttttt attccggatg acgtgtttcc ccgtcgcctt
      841 caaacaacgc gagcatctcc tggtgtgccg ccttgagggc ccgcacttcg gggacttccc
      901 gtccgcccga ctgttgacaa aagatctcga gaatgtccat cccccgggtg ctggccacat
      961 acaacaggcg catcagtctg ggagcacagg ccgcagggtc agccccgtca tccagataat
     1021 gcctgcgctg ctcttcaatt tcgtcaagga gcacaaagac gaccggaaaa tccatcccct
     1081 tggcggtatg gatcgtactg acaaacacgg catccgtttt tgcaaagtcg acatcatcgt
     1141 gcttggcgat cctgaccttg atattccggg cctccaacct ggccgcatag tcttcggggc
     1201 gtccgagcgt caagaccgca atgctccccg gctcgtagag ggcatcatcc cggaacgcca
     1261 gcatcctgat cctctcgaca agatgatcca gcaaaccgtt ccagccggac tcgaaatgca
     1321 catcgggcac gggtcccatc cgggtcgcat gtccggtccc gccctcctcg gcatccggga
     1381 tcatctcgcg caccctgcgg gcaaactcga agatggggat cgtattccgg tagacctgtg
     1441 acagggaaac cgtggtgacc cggcgcatgt ccatgcctgc atcgagaaag ggcgatgtcc
     1501 cccggtagat catctgctcc gaatccccgg ccagcacgag atgttcggcc tgacgcctca
     1561 agatggccag cttggttcct gtcagatcct ggcactcatc gacaaagacc cgtgcccagc
     1621 gtgcccccga ctggccagcc tgcgtgaggt gtccgagcag gagccaccag gccaacgagg
     1681 gcacaccctt gccttcggca agagcctgtt ctaccagttt ttcgtgcaga ctccaaaccg
     1741 cttcgcgttg ggcacgggcc aaagccctgc ccatcccgat ccggctgtac tccagatacg
     1801 actcccgtgt catcacccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     1921 cctgcccatc ccgatccggc tgtactccag atacgactcc cgtgtcatca ccccgcgggt
     1981 ccagagcatt tccagttcgg cctccacacc cgccaggccc tcaatcgcgc ccatccttgc
     2041 aaccagatca gccagcctgg ctgtccagtc aacatcaagc cgcacgtgct ttttcaccag
     2101 accagccagc caggaatcca ccgttttcat ctcgacctgg tccccctcga acatctcgtg
     2161 gaccttgatc tggtactcgc tgtaggcctg gacccgtttt ccccagttca tcacaagtgg
     2221 ccgggagtcc ggtccctcca gaagctcacc ctgcccggcc agctcaagtg cccgggccac
     2281 gcacttgagg aggacaatgg tcttgcccga gccggccggg cccttgacca gaaacaccgg
     2341 ctggtcatgg aactgtcgca cagcctcttc ctggtcgaaa ccgagttgca tcaggttccg
     2401 ctcaagcagg accctggtcc gggagtgaca agccatcgaa cgatagaacg cctcccgctt
     2461 gctcacacga tcgatcaggt tttgcagttc aagaatcttt ttcttgtggt ccgtcatctc
     2521 ggccctgagc tgatccacgc gcccggcctt tgaatccagc cgggccccct cgtcatgcaa
     2581 gcgctggatc tcgcgttcct tatcccgcac atccgcctga agttcatcca gtcgggtctg
     2641 gagacttgcc agccctttct cctccgcgac agccaggctc tcccggatct ccctgttctc
     2701 ggccctgagc tctaccagat cccgcagcag gttctggtag tcaagggggc ctgtctgccg
     2761 caaggcgaca agccggtccc tgatggcagg atcgacgccc gcattctgca tgaacgtgac
     2821 gagggtacag agataatcat cagcccgttc tctccggatg gttgcaaagg catgccggac
     2881 cgtattggtc tcctggtgca tgtatttgac cgttgccaga agctccatcg aaccggccca
     2941 acgccgggac atggcgggat cggagccgat cacccgccgc agttcgtcca gataatcata
     3001 aaagctgatc gtacagatcc cgtccggcct ggtgaacctg gatcgtaccc gaggcatcac
     3061 ttcctggcac caggcttcca tccaggcatg ggtcgcaata cagaagaatc ccggatcggc
     3121 atggtacagc ttttccagtc ttgttgcatc cttgtcatga tccatgcagg gctcctcacg
     3181 gcggcggcat gcctgccgcg cacataatat tgccttggaa tatactgaaa acctcaccct
     3241 gcaaccaagg cacaaaaaaa ccgcccagca ccaagcggac ggttgtatat ctgaagactc
     3301 gcgtcaagcc tcgtcatcgg gcgccttgct cccgcgggga ccacgcttcc ggctttcaac
     3361 catggaacgg atcttttcga caggaaccag cttggccagt tgcttgaatt ccttcccggg
     3421 tttgaaaaaa atcaccaccc ggtcatcgac aacaacggcc tcgcccgagc gcggattcct
     3481 ggccttccgg cctttgcgga tcttgtaccc gaacgttcca aacccgcgaa gctccatcgg
     3541 atcgccctcg gagagggaac ccttgacctt ctcgaaaaac gaatcgatca caaaggcaat
     3601 atcctttttt tggacatcaa cctcacggct gatactttcg acgatctccg ccttggtcac
     3661 ctgtccgctc cccgcactcg agatcccgta tcagccacgg acgaatcagg ccttgacaag
     3721 tgaagcaagc ttttttgcca tgcgggcctt tttgcgggcc acggtattct tgtgcagccg
     3781 gccatgcttg actcccttat ccagcaccga aaacagctcg ggcatgaggg cgaccgcttc
     3841 atcctttttg ccctcggtca aggcaacccg ggacttcttg acctgggtgc ggatcttgct
     3901 catctcggac ttgttgcgca tcttgcgtgt gatgctctga cggttggcct tgaggccgga
     3961 atgatatctc ttggacaagg gaacttcctc ctgtatactc acgccggtac cagtcgcatc
     4021 atctcgggac acgacagacc tgcgcctgac gcatatctgc tatcagacac aaaaatagtc
     4081 gcctgcagcc caaaaagcaa aaattttgtc accggaacct gctttccctc cgttggcccg
     4141 aggttgttca ccaaaaaacg ccacaatcgc tgcccagact ccccggtttg gacccgcatc
     4201 tgctggtcgg cgcgcccgag ttccgtatat ttccggcatg aacgaaacac tcccgctgga
     4261 tgaacgcgtc aggaccttgc ccgacctgcc gggtgtctat ctgatgaaaa actccgacgg
     4321 gacgatcatc tacatcggca aggcccggcg cctcaaacgg cgggtggcct cctacttcaa
     4381 caaaaatcat gattcggcca agacgcaggc cctggtccgc cagatcgcga gcatcgacta
     4441 catcgtcgcc ggcagcgaat ccgaggcact cctgatcgaa gccaacctga tcaaggcgca
     4501 caccccgcgc tacaatatcc agctccggga ttccaagcgc tacccctggc tcgccgtcag
     4561 catcggggag gattttcccc gctttttcaa aacccggaac cccggcgata ccggtctccg
     4621 caccttcggc ccctggccca atgtttccgt catccgtaat acgatttcca tcctccacag
     4681 gctcttcggg ttccgctatt gccgcaaacg tctggtcgag gggacgcccg agccaccggt
     4741 ctgcttttat taccacatca aacgctgccc cggcccctgc gagggtcaca tcagccgggc
     4801 ggactaccgg gccggcatcg aacgggcaat cctcttcctc gaagggcggc atgacgatct
     4861 gcgggtgatg ctcgaggaac agatgcgggc ccatgcacgc gcgctcgaat tcgaaaaagc
     4921 ggccgccatc cgcgatcaac tggccgccat cgccgaggcc ggaacggtcc aggccgtcca
     4981 tcgcgtcatc gaggagggtg cgcccgccga tctcgatatt gccgccatgg ccgaacactc
     5041 cgggatggcg gcctttgccg tcatggagat ccgtggcggc aaactccttg cccagcacct
     5101 ctttctcgag aatgatgcgg attccaagcc ggagctcttc ttccaattcc tccgggcctg
     5161 gctccagaca caaagctccg gtgacgatcc ccggccactg ctcctgatcc ctgactcctc
     5221 catcgaagac gagcatatcg aagaaggatc tgtcatgaaa cgccgtcttc ttcccgcagg
     5281 ggaggccgac cctggcctgc gccggctcct ggcccaagcc cgttccaatg ccgccctggc
     5341 cctcgaagaa cagatctcgc ggcggctcta cgcggacggc ctgaccgatc tccagaaaat
     5401 cctg
//
LOCUS       DAOSHE010000098         5400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13435_length_5400_cov_4.405987, whole genome shotgun sequence.
ACCESSION   DAOSHE010000098 DAOSHE010000000
VERSION     DAOSHE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13435_length_5400_cov_4.405987"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..749)
                     /locus_tag="PLD82_03995"
     CDS             complement(180..749)
                     /locus_tag="PLD82_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG64580.1"
                     /translation="MLQKRKALRLAGYNYATPGAYYITICTHGRKSIFGMIEDRTMNL
                     NDYGRIVEKCWFNLPDHYPNIQLGEWIIMPNHIHGIIEIVTVGAGSQTLPNVGAGLQS
                     LPIVRAGLKPAPTTKKHHGIPEIVRAFKTFSSRRINKLRNTPGTPVWQRNYFEHIIRN
                     KKSMQQISNYIRNNPLHWSGDDQCKPDIW"
     gene            955..1410
                     /locus_tag="PLD82_04000"
     CDS             955..1410
                     /locus_tag="PLD82_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG64581.1"
                     /translation="MKSLLSLSEGASLALHGLVLVAKRHPERVSARELALALDASEAH
                     LAKVFQKLHRAGIVDSLRGPAGGFSLARPAGGVSLIEIYQIIDGTVHPGACPLGKTKC
                     LFRECIFTGGINGILAELHDKLASITLADVIGHDGPEAVQQKTRKAVRS"
     gene            1429..2649
                     /locus_tag="PLD82_04005"
     CDS             1429..2649
                     /locus_tag="PLD82_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG64582.1"
                     /translation="MARFESSRTLFDICETHPEVVSVFVSNGFPQMSDPEKRKAFGKQ
                     ITLGQALALKGLDPAVFAGLLEAALARAAGLCESGLKHEAAAEIEVTGLLPCPVRVPL
                     LEEFEGFVATLASEQGTRVNYELKAASQGLDWMGHRLEGPADDLPDVFLSAGFDMFFD
                     RQRFGRFRDAGVFEDLVSYQGVNPLFADVGLRDPRKTYSVIAAVPAVFLVNLDELGER
                     PLPRRWSDVLSPEFEQRVSLPVGDFDLFNAILVNIFKAYGDEGVRRLGRSLLESMHPS
                     QMVRSGKKEGARPIVTIMPNFFTKMVREGSGMQAVWPEDGAITSPVFMLTKKSKAGEL
                     QPLVDFFAGKAAGEIMAHKGLFPSLHPEVDNRMGSGLPFLWPGWDWLDSQDIGGLIRH
                     GMQVFEQALLEVSA"
     gene            2646..3359
                     /locus_tag="PLD82_04010"
     CDS             2646..3359
                     /locus_tag="PLD82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG64583.1"
                     /translation="MNLVTISGPPSSGKTSVILKTAAALMSRGLAVGVVKFDCLYTDD
                     DELYRKAGIPVKKGLSGQLCPDHYFVSNIEAVADWGKKSGLDLLISESAGLCNRCSPY
                     ISGIKAVCVIDNLSGINTPKKIGPMLKMADIVVITKGDIVSQAEREVFASRVNAVNPG
                     AVIMHINGLTGQGSYELSTLLYSVEAGFATLKGMKLRFSMPSALCSYCLGETRIGEEH
                     QLGNVRKIDLHEEEPHAAQ"
     gene            3346..4371
                     /locus_tag="PLD82_04015"
     CDS             3346..4371
                     /locus_tag="PLD82_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG64584.1"
                     /translation="MLHSDDIAGLAVADLLARFPFMEAFFRDNGIVTAGRERIRLADL
                     IGEREAEDRGTDGNRLAAQAAEYINRMLAFLGEGEQVTRITILPGRDKSGQPEGFETL
                     EIRTSEIISIVGPTGSGKSRLLADIEWVAQADTPTGRRVLINGAPPDPKWRFSSADKL
                     VAQLSQNMNFVMDLSVREFVALHAESRMIRDADSVVERIIAQANELAGEPFTPETAIT
                     ALSGGQSRALMIADTAILSKSPIILIDEIENAGIDRQRALALLLSEEKIVLMATHDPV
                     LALMADRRLVIKNGGIASVVETTEQERSILSELRALDAQVLEYRARLRRGERLDTAKE
                     GKEAVHA"
     gene            4364..4606
                     /locus_tag="PLD82_04020"
     CDS             4364..4606
                     /locus_tag="PLD82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64585.1"
                     /translation="MHDGCSGSFGDGKAVVDKIRAMGFAPQFMPIPLQILCGGCGKPF
                     DMVHFEEACPDCGMVHGVTPCHSYDPQAVQAAGIGY"
     gene            4742..5377
                     /locus_tag="PLD82_04025"
     CDS             4742..5377
                     /locus_tag="PLD82_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64586.1"
                     /translation="MGMFCYQCQEALKNTGCTVNGVCGKKGDTANLQDLLVYTAKGVA
                     LAALASGKVSEEAGHYLVKALFTTVTNVSFDNAQLIGFVREGLRLRDSLKSAQSGHDA
                     LVWVPGDDAALLAKAETIGVLATENPDARSLRELLVYGMKGIAAYADHALVLGYEDPA
                     IAQFLAEGLASTTQDLSLDAMIGLVMKAGEVAVTTMALLDKAIPKPTEIPS"
BASE COUNT         1126 a         1455 c         1675 g         1144 t
ORIGIN      
        1 atggagaaac gcggtagggt tgaaacccgc ccctacgaag gccggaccat gtgaacgcgc
       61 cacgacgatg ccggacgttt gcaatcccgc aacgatgaca ttggcccatg tgaacgcgcc
      121 acaacgatat aggacaatca ccttttcccg tcggggcccc acaatggtgg tgtatgcgat
      181 taccatatat ccggtttgca ttgatcgtca ccggaccaat gtaagggatt gtttctgata
      241 taattggaga tctgttgcat ggattttttg ttgcggatga tgtgttcgaa ataattacgt
      301 tgccagacag gggtgccggg tgtgttgcgt aatttattga tgcgacgtga tgaaaatgtt
      361 ttgaacgcac ggacaatttc tggaatacca tgatgttttt tggtcgtagg ggcgggtttc
      421 aaacccgccc ttacgatggg caacgattgt aaacccgccc ctacgttggg caacgtttgt
      481 gaacccgccc ctacggtaac gatttcaata atgccgtgaa tgtgatttgg cataatgatc
      541 cattcgccca attgtatatt cggataatga tcgggcaaat tgaaccagca tttttctacg
      601 atgcggccgt aatcattcaa attcattgtt ctgtcttcaa tcatgccaaa aattgatttt
      661 cggccatgcg tgcagatagt tatataatag gcacctggtg tcgcgtaatt atagccggcc
      721 aatcgcaatg ccttgcgttt ttgaagcatg gtgtgctcct tattgttcaa tgctacaata
      781 ccattaccga tgtgtataga cggatgtatc ccccggcgtg ttcgacctca tccaccagac
      841 cggaggctgt tccccataaa gcgccataac gcagcaaaaa aatcagaaaa aaacttgcat
      901 accctgccat aaaaaggtat aatagtaccg aaatgcaaga atacggaggc ggcgatgaag
      961 agcctgctga gtctgtccga gggtgcctcg cttgccctgc atgggctggt cctcgtggcc
     1021 aagagacacc ctgagcgggt cagtgccagg gagcttgcgc tggccctgga tgcctccgag
     1081 gcccatctgg ccaaggtctt tcaaaaactc caccgggcgg ggattgtgga ttcgctgcgc
     1141 ggcccggctg gcgggttttc gctggccagg cctgccgggg gagtctcact gatcgagatc
     1201 taccagatca ttgacgggac tgtccatccc ggggcctgtc cgctgggcaa gaccaagtgc
     1261 ctctttcgcg agtgcatctt tacaggcggg atcaacggga tcctggccga gctgcacgac
     1321 aagctggcca gcattaccct tgcggatgtc atcggccatg acgggcctga ggctgttcaa
     1381 caaaaaacac gaaaagcggt cagaagctga tcgcggaaag gatatacgat ggcacgattt
     1441 gaatcatccc ggaccctgtt tgatatctgt gaaacgcatc ccgaggtggt cagcgtcttt
     1501 gtctccaacg ggtttccgca gatgagtgac cccgagaagc ggaaggcatt cggcaagcag
     1561 attacccttg gacaggccct ggccctgaag gggctggacc cggccgtgtt tgcgggactg
     1621 ttggaagcgg ccctggcccg ggcagccggt ctctgcgagt ccggcctcaa gcacgaggcg
     1681 gccgccgaaa tcgaagtcac agggctcttg ccctgtcccg tgcgggtacc tttgttggaa
     1741 gagtttgagg gttttgtggc gaccctggcc agcgagcagg ggacacgcgt caactacgaa
     1801 ctcaaggcgg cttcgcaggg gctggactgg atgggccatc gtctcgaggg cccggcggac
     1861 gatctgccgg atgtcttttt gtcggccggg tttgacatgt tttttgaccg ccagcgcttt
     1921 ggccgttttc gggatgcagg tgtttttgag gaccttgttt cgtaccaggg tgtcaatccc
     1981 ctgtttgcgg atgtcggcct gcgtgatccg cgcaagacct actcggtcat agccgctgtc
     2041 ccggccgtct ttctggtcaa tctggacgag ctcggggagc ggcccctgcc acgccgctgg
     2101 tcagatgttt tgtcaccaga atttgaacag cgggtatcat tgccggtcgg tgactttgac
     2161 ctctttaacg ccatccttgt caacattttc aaggcttacg gggatgaggg cgtgcgccgg
     2221 ctcggtcgca gcctgcttga gtccatgcat ccctcccaga tggtcaggag cggcaagaag
     2281 gagggggcac gaccgatcgt caccatcatg ccgaacttct ttacaaaaat ggtgcgcgag
     2341 gggagtggca tgcaggccgt ctggcccgag gacggggcga tcacgagccc ggtctttatg
     2401 ctcaccaaaa aaagcaaggc gggggagttg cagcccctgg tggacttttt tgccggcaag
     2461 gctgccgggg aaatcatggc ccacaaggga ctcttcccca gcctgcaccc cgaggttgac
     2521 aaccggatgg gttcaggctt gcccttcctc tggcccggct gggactggct ggacagccag
     2581 gatatcggcg gcctgatccg ccacgggatg caggtgtttg aacaggcgct cctggaggtg
     2641 tcggcatgaa tctcgtgacc atctccgggc ctccttcgtc gggcaagacc tccgtcatcc
     2701 tcaagaccgc cgccgccctg atgtcccgcg gcctggccgt gggcgttgtc aaattcgact
     2761 gcctgtatac cgatgatgac gagctctacc gcaaggcggg gatcccggtc aaaaagggtc
     2821 tctccggcca gctctgtccg gatcactact ttgtcagcaa tatcgaggcc gtggccgact
     2881 gggggaagaa gagcggactg gatctgttga tcagcgaatc tgccggcctc tgcaaccgct
     2941 gctcgcccta tatcagcggg atcaaggcgg tctgcgtgat cgacaatctc tccgggatca
     3001 acacaccaaa aaagatcgga ccgatgctca agatggcgga catcgtggtg atcaccaagg
     3061 gtgatatcgt cagccaggcc gagcgcgagg tctttgcctc acgggtcaac gccgtcaatc
     3121 cgggcgccgt catcatgcac attaacggct tgaccgggca gggcagctat gaactctcga
     3181 ccctgctcta ttcggtcgag gccgggtttg ccaccctcaa gggcatgaag ctgcgttttt
     3241 cgatgccatc ggcactttgc tcgtactgcc tgggtgagac caggatcggt gaagagcacc
     3301 agttgggtaa tgtgcgcaag atcgatcttc acgaggagga accgcatgct gcacagtgac
     3361 gacattgccg gtcttgcagt ggcagacctg ctggcccggt ttccgtttat ggaagcgttt
     3421 ttccgcgaca atggtattgt gacggctgga cgcgagcgca tcaggttggc ggatctgatc
     3481 ggggagagag aagctgagga ccggggaacc gacggaaaca ggctggcagc gcaggctgcc
     3541 gaatacatca accggatgct ggcatttttg ggcgagggcg aacaggtcac caggatcacg
     3601 atcctgcccg gacgcgacaa gtcgggtcag cccgaggggt ttgaaacgct cgagatccgg
     3661 accagcgaga tcatctcgat tgtcggtccg accggaagcg gaaaaagccg gctgttggcg
     3721 gatatcgagt gggtggccca ggcggatacc ccaaccgggc gcagggtgct gatcaacggg
     3781 gcacctcccg atcccaaatg gcgcttttcg tcggccgaca agctggtggc ccagttgtcg
     3841 caaaacatga attttgtcat ggatctttcg gtgcgcgaat ttgtcgccct gcatgccgaa
     3901 agccgcatga tcagggatgc agactcggtg gttgaacgca tcattgccca ggccaacgag
     3961 ctggcgggcg agccctttac cccggaaacc gccatcacgg ccctctcggg cgggcagtcc
     4021 cgggccctga tgattgccga taccgccatc ctgagcaagt cccccatcat cctgatcgac
     4081 gagatcgaaa atgcggggat cgaccggcag cgtgcgctgg cactcctgct ttcggaggaa
     4141 aagatcgtcc tgatggccac gcacgatccg gtcctggccc tgatggccga caggcgcctg
     4201 gtcatcaaaa acggggggat tgcctcggtt gtggagacaa cggaacagga gcgttcgatc
     4261 ctgagcgaac tcagggccct ggatgcacag gtgttggaat accgggccag actgcgccgc
     4321 ggggagcggc tggatacagc gaaagaaggg aaggaggccg tgcatgcatg atggctgtag
     4381 tggaagtttt ggtgatggca aggcggtggt ggacaagatc agggcgatgg ggtttgcgcc
     4441 ccagttcatg ccgatccccc tgcagatcct ctgcggaggg tgcggcaagc catttgatat
     4501 ggtacacttt gaagaggcct gcccggactg cgggatggtg cacggcgtga cgccctgcca
     4561 ttcgtatgac cctcaggcgg tgcaggccgc cgggataggg tattgatcgg ggtttttgca
     4621 gggacaggct gcgaccagga gcttttatat ggaaagaaca ggtcgcaacc catccataac
     4681 aatacggcgc agtacggtat gggcgggtct gtgacccgtc cggtagaaag gagaatcaat
     4741 catgggaatg ttttgctatc agtgtcagga ggccctcaaa aatacggggt gcacagtcaa
     4801 cggtgtctgc gggaaaaagg gagataccgc caacctgcag gatctcctgg tctacacggc
     4861 caagggtgtg gccctggcgg cgcttgcttc gggcaaggtc tctgaggagg ccgggcacta
     4921 cctggtcaag gccctgttta caaccgtgac caatgtcagc tttgacaatg cccagttgat
     4981 cgggtttgtc cgcgaagggc tgcgcctgag ggacagtctc aagagcgcac agtccgggca
     5041 cgatgccctg gtctgggtgc cgggtgatga cgcggcgctg ctggccaagg ccgaaacaat
     5101 cggggtcctg gcaaccgaaa atcccgatgc ccgttcgttg cgcgagctgc ttgtctatgg
     5161 catgaagggg attgcggcct atgccgatca tgcccttgtg ttaggctacg aagatccggc
     5221 catcgcccag tttcttgctg aaggtctggc ctcaacgaca caggacctct cgctggacgc
     5281 gatgatcggg ctggtgatga aggccggtga ggtggccgtg acaactatgg ccctgctgga
     5341 caaggcaata ccgaaaccta cggaaatccc gagctgaccc aggtcgatat cggggtcgga
//
LOCUS       DAOSHE010000099         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13490_length_5384_cov_4.121223, whole genome shotgun sequence.
ACCESSION   DAOSHE010000099 DAOSHE010000000
VERSION     DAOSHE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13490_length_5384_cov_4.121223"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLD82_04030"
     CDS             <1..506
                     /locus_tag="PLD82_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG64587.1"
                     /translation="GVTMAVLEALKEAILSERRAAAFYETAAKNAGDAGVRSVFSTMA
                     EEERGHARLLAKQWASAVQGGNFSAPSDEPMQPGAQDAIVSHAIRNGLLAAGFEAGAI
                     AAAMALEERAIKFYGARAEVATDPNEKALYCWLATWEKGHLEFLAKMDDQLRETVWHD
                     QNFWPLD"
     gene            complement(579..1853)
                     /locus_tag="PLD82_04035"
     CDS             complement(579..1853)
                     /locus_tag="PLD82_04035"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG64588.1"
                     /translation="MYETIALVLGGGKGTRLYPLTKDRSKPAVPIGGKYRLIDIPISN
                     CLNSNINCIYIVTQFNSASLNKHINQAYRFDNFHEGFIDILAAEQSNESSDWYQGTAD
                     AVRKNLKHLLSFPQLENVLILSGDQIYTMDYSRMMEFHKANKCDMTLAVIPVVEKDTA
                     GFGIMKLDDYRVTDFIEKPKTPEARAGYANINLVKKYFPDIDPKKEYLASMGIYLFSR
                     EALVKLLDNDKTDFGKDLIPQAIRNMNVGGYLFDGYWEDVGTIDSFIKANIDFAADHA
                     SFDFYESKVYTNSRFLPGSRVCDSVVKQSLLTDGIIVGKGTTIERSVIGIRSIIGSNV
                     TIEGSYVMGADWYEIPEHKDYNRKHQILDIGIGDNTVIKNAIIDKNARIGKNCKIVNE
                     KNLQEFSGPNYNIVDGVIIVHKNATLPDGSVI"
     gene            2046..2291
                     /locus_tag="PLD82_04040"
     CDS             2046..2291
                     /locus_tag="PLD82_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64589.1"
                     /translation="MAGMQVYVEFIGPIARGDWAAKQDFSLDVGTVLRDFLVSIGYER
                     EHVEHIIVVRNGKITKDLETVLEDGDHLSLSVLLGGG"
     gene            complement(2363..2764)
                     /locus_tag="PLD82_04045"
     CDS             complement(2363..2764)
                     /locus_tag="PLD82_04045"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG64590.1"
                     /translation="MDQAQKKALVDAAIKAAEKAYAPYSHFRVGAALLDEKGTVYTGC
                     NIENASYGLCNCAERTALFKAISEGVTPKTGIKALAVVTLDAAGFCSPCGACRQVIHE
                     LATQAMILLADRNGTWQETTPDELLPGAFVL"
     gene            complement(2790..3812)
                     /locus_tag="PLD82_04050"
     CDS             complement(2790..3812)
                     /locus_tag="PLD82_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64591.1"
                     /translation="MATKRPIAPKAAPTATQPQKSSRNFILGALCVILLLSGIQFLLL
                     HTVSSPTLKPLDPKNEIRLQVVRILDLDFPFQSDAEFSSFLKLLSETVKKKTGYTVVF
                     EENRKIISDEFTGGNDIFADSKAAEAWFASQLAGSRGWQTNFSWLKPVLQNPVSRKIL
                     ESWYGTNDLSGQIVKDFSRKISSLIALKGVRGKRVFDDPRRHITSSTAWWHYLLGKQA
                     EGDLIVANYPIFYPSALTPVDAITRGGLVTSMLAPSSRPLGATIGLSSWPVYGRRQFH
                     PETAQKIFVELGAQALGRVLLRRGFDLENNNSLLYPVLGEDLLKWYNKGSREPGRASS
                     TPLQRF"
     gene            complement(3842..>5384)
                     /locus_tag="PLD82_04055"
     CDS             complement(3842..>5384)
                     /locus_tag="PLD82_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOG64592.1"
                     /translation="DGVLVTTAAGEVILISSSGKTLWTRRLAAPVIRPPIIWRGSAWV
                     LAGRGILCLDLSDGRTIWSRNESAATQLVAPAVSQGILFHGTRTGIQARIATNGQPVW
                     TNPSISAWGAALVVIEDNLLVQHRNYRTRSSFLACLDTGSGATRWTREIPHEANIFAP
                     LVVGERVFQAAHDTLAVFSLRDGTSITNIRFSAPLASHPAATGPLLILPLTDGTIVTL
                     SSTTFSVQTTFSHLRRQGNILAVSSQYLYCATDSGSIAELSVTNGKVGRSLPLPAAAS
                     HAQPFLRDGLLYVPSQTTLICIGPPHEARPDTDTGRTSVQRILVLLDARTKNPLPGIA
                     RIAWQDGHAGIREAGIGFDGSGSAILPESIPVNDISLTIQRRGYVYTNSTWPRNTGRL
                     VVHLEPIDAQTRLILNDILFDTGSASLLPQALPSLHRLARFMQANPALTVLIEGHTDD
                     VGDRETNLALSLARAGSIREYLVRQGIGGWRIRTTGFGPDRPVAPNTTAEGRMRNRRT
                     EIRIL"
BASE COUNT         1054 a         1320 c         1758 g         1252 t
ORIGIN      
        1 gaggtgtcac gatggcagtt cttgaggcat tgaaggaagc gatcctttcg gagcggcggg
       61 ccgcggcgtt ttatgagacg gcggcaaaga atgccggcga tgcaggggtg cgttcggttt
      121 tttcgacgat ggcagaagag gaacgcgggc atgcccggct tctcgcgaaa cagtgggcca
      181 gtgcggtcca gggcgggaat ttttcggcac cttccgatga gccgatgcaa cccggggccc
      241 aggatgcgat cgtcagccat gccatccgca atggcttgct ggcggccgga ttcgaggcgg
      301 gggcgattgc cgccgcgatg gcactcgagg agcgggcgat caagttctat ggggcccggg
      361 ccgaggtggc cacggatccc aacgaaaagg cgctctattg ctggctggca acctgggaaa
      421 agggacatct tgagtttctg gccaagatgg acgaccagtt gcgcgagact gtctggcatg
      481 accagaattt ctggccgctt gattgaagcg ctctccggcc ggagagaaaa aaagactgcc
      541 cggaagccgg acagtctttt ttgttgtggt gcgcccggtc agatcacgga tccgtccgga
      601 agcgtcgcgt ttttgtggac aatgatcacg ccatccacga tattgtaatt cggtccagaa
      661 aattcctgaa ggttcttctc gttgacgatc ttgcagtttt ttccgatgcg ggcatttttg
      721 tcgatgatgg cgtttttaat gacggtattg tcaccgatcc cgatgtcgag gatctggtgc
      781 ttgcggttgt agtccttgtg ctccggaatt tcgtaccagt cagcgcccat gacgtaggat
      841 ccctcgatgg tgacattgga tccgatgatg ctccgaattc cgatgaccga acgttcgatg
      901 gttgtcccct tgcccacgat gatcccgtct gtcagaaggg attgtttgac gaccgaatca
      961 cagacgcggg atccggggag gaaccgtgaa ttggtgtaga ccttgctttc gtagaagtca
     1021 aaggaagcat gatctgcggc aaaatcaata ttggccttga tgaaggaatc gatggttccg
     1081 acgtcttccc agtagccgtc aaagaggtac ccgcctacat tcatgttgcg gatggcctgg
     1141 gggatgagat cctttccgaa atccgtcttg tcgttgtcca ggagtttgac cagggcttct
     1201 ctgctgaaaa ggtagattcc catggaagcg aggtattcct ttttgggatc gatatccgga
     1261 aagtactttt tgaccagatt gatgttggcg tagccggctc tggcttcggg ggttttcggt
     1321 ttttcgatga agtcggtcac cctgtagtca tccagtttca tgattccgaa tccggctgta
     1381 tccttttcga cgacaggaat gacggccagg gtcatatcgc acttgttggc cttgtggaat
     1441 tccatcatcc tggaataatc cattgtatag atctggtcac cgctcaggat caggacattt
     1501 tcgagttggg gaaacgacaa aaggtgcttg aggtttttcc ggacggcatc agccgtcccc
     1561 tgataccagt cactgctctc gttggactgt tcggcggcaa ggatgtcaat gaagccttcg
     1621 tggaagttgt cgaagcggta ggcctggttg atgtgcttgt tgagtgatgc cgaattgaac
     1681 tgggtcacga tgtagatgca gttgatgttg gaattgagac agttgctgat cgggatgtcg
     1741 atcaggcggt acttgccccc gatcggaacc gcaggctttg aacggtcctt ggtgagagga
     1801 tagagtcggg tgccttttcc gccgcccaga acaagggcta ttgtttcgta catggtacat
     1861 ccctccttgt gattcgcgtt gattgtacag cactggtcgt gccgctgttt tccttcagat
     1921 gcccattcta gcacaggtca gaggaaaaag tgaacaggaa aatcccgaaa ggagccaggg
     1981 gcgtgatctc tgtatctctc cggcagtccg tgtgtatgtt tttgcataga accggaaggg
     2041 gagggatggc tggaatgcag gtgtatgtcg agtttatcgg tccgattgcc cgtggtgact
     2101 gggcggcaaa gcaggatttt tcgttggatg tggggacggt tctgcgcgat tttttggtct
     2161 cgatcgggta tgagcgggaa cacgtggagc acatcatcgt tgtccggaac gggaaaatca
     2221 ccaaggatct ggagactgtt ctcgaggacg gggatcatct ttccctgtcg gttcttttgg
     2281 gcgggggatg atgtccgggt ctggttgaac aagccccgct cttccggttt ctcttgggcg
     2341 agcggggttc ttgtcgttcc ggtcagagca cgaaggcgcc cgggaggagt tcgtcggggg
     2401 ttgtttcctg ccatgtcccg ttacggtccg caagcaggat catggcttgt gttgccagct
     2461 catggatgac ctggcggcag gcaccacagg gcgagcagaa tccggcggca tccagcgtga
     2521 cgacggccaa ggccttgata ccggttttag gggtgacccc ctcgctgatg gccttgaaga
     2581 gagcggtgcg ttcggcgcaa ttgcagagac cgtaggatgc attttcaata ttgcacccgg
     2641 tgtagacggt tcctttttcg tctaacaacg ccgcaccaac ccggaagtgc gagtagggag
     2701 cgtaggcttt ttcggcggcc ttgatggctg catccaccaa tgcttttttc tgggcctgat
     2761 ccatgaggta ctccttgtct gggtgatatt cagaagcgct gcaacggggt gcttgaggcc
     2821 ctacccggct cgcgtgaacc tttattatac catttcagca ggtcttcacc aagcacggga
     2881 tacaacaggg agttgttgtt ttcgaggtcg aacccgcggc ggaggagaac ccgtcccagg
     2941 gcttgtgcgc ccagttcgac aaagatcttt tgggcggttt ccggatggaa ttgccggcga
     3001 ccgtagaccg gccagctcga gaggccgatg gtcgcaccga gggggcgact ggatggtgcc
     3061 agcatcgacg tcaccaaccc gccacgggtg atggcatcga caggggtgag tgcggaggga
     3121 tagaagatcg ggtaattcgc gacgatcagg tctccttcgg cctgcttgcc aaggagatag
     3181 tgccaccagg cggttgaaga agtgatatga cgcctcggat catcgaagac acgttttccc
     3241 cggacgccct tcaaggcgat cagcgacgag atctttcgcg aaaaatcttt gacgatctgt
     3301 cccgacaggt cattcgttcc gtaccatgat tcgaggattt tccgtgagac aggattctgg
     3361 aggacgggct tgagccagga gaaatttgtc tgccaaccac ggcttccggc gagttggctg
     3421 gcgaaccatg cctctgcggc cttgctgtcc gcgaagatgt cattcccgcc ggtgaattcg
     3481 tcgctgatga ttttgcggtt ttcttcaaag acaacggtgt acccggtctt ttttttgacg
     3541 gtctccgaga ggagcttcaa aaatgatgaa aactctgcat cgctttgaaa gggaaagtcg
     3601 aggtcaagga tgcggacgac ctgaaggcgg atttcgtttt ttggatcgag cggtttgagt
     3661 gtcggtgaac tgacagtgtg caggaggaga aactggatgc ctgagagaag gaggatgaca
     3721 catagggcac ccaggatgaa attccggctg ctcttctgcg gttgtgttgc tgtgggtgct
     3781 gcttttgggg caatgggcct ttttgtcgcc ataggaaacc tctgtcgcgg tggaatgcca
     3841 gtcaaagtat acgaatttct gtgcgcctgt ttcgcattcg gccttcggcg gtggtgttgg
     3901 gtgccacagg gcgatccgga ccaaaccctg ttgtgcggat ccgccagccg ccaattccct
     3961 ggcgaacgag gtattccctg atgcttcccg cccgggccag ggagagtgcc agattggttt
     4021 cgcgatcgcc gacatcgtcc gtgtgtcctt cgatcaggac cgtgagtgcg gggttggctt
     4081 gcatgaacct ggccagccgg tgcagggatg gcagcgcctg tggaagcagg ctggcactcc
     4141 cggtatcgaa gaggatatcg ttcaggataa gacgggtctg tgcgtcgatc ggttcaagat
     4201 ggacgacaag acgtccggtg ttgcgcggcc aggtggaatt ggtatagacg tatccccggc
     4261 gctggatcgt caggctgatg tcattgacgg ggatactttc cggcaggatg gccgagccgc
     4321 ttccgtcaaa cccgattccg gcttcccgta tcccggcgtg tccatcctgc caagcgatcc
     4381 gggcaatccc gggtagcggg ttttttgtcc gggcatcgag gagcaccagg atgcgctgga
     4441 cacttgttcg cccggtatcg gtatcgggtc tggcttcatg aggcggaccg atgcagatca
     4501 gggtcgtctg gcttggaacg tagagcagcc cgtcccggag aaagggctgc gcatggcttg
     4561 ctgcagccgg gagaggcagg gaacggccaa ccttgccatt cgttacactg agttcggcga
     4621 tgctcccgga atccgtggcg caatagaggt actgactgct gacagccagg atgtttcctt
     4681 gacgacggag atggctgaac gtggtttgga cggagaaggt cgtgctcgag agggtcacga
     4741 tggtcccgtc cgtcaaggga aggatgagga gggggccggt cgcggccggg tggctggcga
     4801 gcggtgctga aaaccggata tttgtgatgg atgttccgtc ccggagggaa aagacggcca
     4861 gtgtgtcatg ggccgcctgg aacacccgtt ctccgaccac aaggggagcg aagatattgg
     4921 cctcgtgggg gatttcccgg gtccagcgtg ttgcgccgga tccggtatcc agacaggcca
     4981 gaaacgacga gcgggtgcgg taattgcggt gctggacaag gagattgtct tcgatcacga
     5041 ccaaggccgc accccaggcc gagatggagg ggtttgtcca gacgggttgg ccattggtgg
     5101 cgatgcgggc ctggattcca gttcgcgttc catggaagag gatcccttgg gagacggccg
     5161 gggcgaccag ctgggtggcc gcactttcgt tgcgggacca gatggtccgg ccatcggaga
     5221 gatcgaggca gaggatgccg cggccggcga ggacccaggc cgagccgcgc cagatgatgg
     5281 ggggacggat gaccggggcg gccaggcgtc tggtccagag cgtttttccg gatgatgaaa
     5341 tcaggatcac ctcgccggca gcggttgtga cgaggacgcc atcc
//
LOCUS       DAOSHE010000100         5378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13511_length_5378_cov_4.998873, whole genome shotgun sequence.
ACCESSION   DAOSHE010000100 DAOSHE010000000
VERSION     DAOSHE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13511_length_5378_cov_4.998873"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLD82_04060"
     CDS             complement(<1..214)
                     /locus_tag="PLD82_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64593.1"
                     /translation="MTVQKMQTGAAIFLALFALLPLFGHMESNDNHKNYANHNYGKNL
                     MNTLEPNSVFMTEGGDNQVFTSAYNQM"
     gene            complement(214..2310)
                     /locus_tag="PLD82_04065"
     CDS             complement(214..2310)
                     /locus_tag="PLD82_04065"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOG64594.1"
                     /translation="MNLIGNSDFGLKHGMRLPIWRFERIDWMIAGAAFLLAFVVYTLT
                     MTPEICAGDSGELTTAIYNLGAAHPPGYPLYTMLGKVFTYVPAGSVAFRVNLLSVFFG
                     ALTIPFLYLLIMRLMHRGAEAGLALRDRVISFGATMAFAFSSTMWSQAVIAEVYTLNA
                     IFAPMILLAVLVWQEEVYASIANDRPSIGERLFLLIALLLGMSFTNHLLLVGYVPPLV
                     FFFMIVTQHIRGGASGAGNTKVLTPLVYIGTAASLGFGLMLALFADGLPSAVFAVLLV
                     VLSLIPAGVAFVSWIASMKTVSTNKETYHASKIVVCIGIGITSLIAAGLFWSNAWTTS
                     LLDEKHAARTLIAVLLPLIALGVSSIVMLSRLPLARASAPSRANLWTGLALVGWTFLF
                     LLVVAEKFIMSLLGLLLFFLPGATFQGSRIRESLDFIGVGWGFDLFVTLLWLLGVTII
                     VYTWLQSRRKCTADEQFTATLTLLFKGALFFLLPLMLYMTLLIRANAIAKIPDPPLSW
                     GETTNASRVINHFLRKQYPKASMYFSNRIFEITNGWLRMHVHQYIPLVKHETPPKTEI
                     KIENGQTNTIRHPATMMESGSWAPRDLLLALPVFLLLTIVGLVGLYRHNPLWFWLLLS
                     IFATFNVLLLTMLSPRDTARDWFFNEVFFIPSHVIVAVWMAFGMRGIAGWIARLFGKA
                     RPQAVPAGSGSEGGDA"
     gene            complement(2391..2549)
                     /locus_tag="PLD82_04070"
     CDS             complement(2391..2549)
                     /locus_tag="PLD82_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64595.1"
                     /translation="MTRSDGQWRIRSELSTLVRNENSYPSRIHLPVLACESILFSIVS
                     VIQYIRLP"
     gene            complement(3062..4573)
                     /locus_tag="PLD82_04075"
     CDS             complement(3062..4573)
                     /locus_tag="PLD82_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOG64596.1"
                     /translation="MKSCRMNWPSFCALLILVCLLIPAGLRGARVWESGMGDRFIGAS
                     LDNLRLVNDTDGTEALRLADNQAEDDDSTDLLLHGNKPDDFRVKNYGIDSSSVVIDTI
                     LPLFGAGSMRFPGPDTRLLLTPRTGALLGEVTDMGSFTIDFWIWPYSQYDEEDVFSRY
                     GNLLAADGRVLETGMRIFFRNNRLVFLLKNMLHGRGERVHDAELVGQRVSRLRVWQHV
                     AFIYDDASGKLSRQVDGVEDGIVWLTDTGRQGGTPLRPAFLKGFRRQAQLGGGFRGRI
                     EDFRIRRGAWVHSETGRYSTAIGRITSRVVDMGTDNAKMESASWKSETPAGSAILLEY
                     RISGEIFPADDGEIPWIRLRNPTTGFEIPRGRFVQWRAILVGSERGRYTPVLKSVRLV
                     WDPAWRPDTPVQLTARPGDGRIILRWRANMDRIAGYRIYLGTERGEYLHPDSPIDVPL
                     SRLDPANPSYTLRNLENDRLYYIVVTAYTKDGMQSGFSREVHARPDETAGGRE"
     gene            4744..>5378
                     /locus_tag="PLD82_04080"
     CDS             4744..>5378
                     /locus_tag="PLD82_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG64597.1"
                     /translation="MEGIRVLMAAAEAAPFAVLGELAQVMGSLPYYLKNLGCNVRVIL
                     PYYPSVANASPVTPMPIGEIRFTMNGREERAIIRGTERHGVLFYFVERDDLYGREGLY
                     QYRNDDGTMQDYEDNLYRFAFFSRAVLEASAVTGFYPRIIHCNSWHTALVPVYLKTIY
                     ARSSAHSKTSTLLTIPNMAFQGLFPSAQYNTTGLPWEVFNTNALEYYGMINL"
BASE COUNT         1310 a         1552 c         1435 g         1081 t
ORIGIN      
        1 ccatctggtt ataggccgag gtgaacacct gattgtcccc gccctcggtc atgaagaccg
       61 agttgggctc aagggtattc atcaaattct tgccatagtt atggtttgcg taatttttgt
      121 ggttgtcatt ggactccatg tggccaaaga gcggcaacaa ggcaaagaga gccaggaaaa
      181 tggccgctcc cgtctgcatt ttttggaccg tcattatgcg tctcctcctt cactgccact
      241 tccggccggc accgcttggg gacgtgcctt tccgaacaag cgggctatcc agccggcaat
      301 cccccgcatc ccgaaagcca tccagacagc cacgatgaca tgggatggta tgaaaaagac
      361 ttcgttgaaa aaccagtcac gagccgtatc gcgaggagaa agcatggtca gaagcagcac
      421 attgaaggtc gcaaaaattg acaagagcag ccagaaccag agcggattat gccgatacaa
      481 accgaccaac cccacgatgg tcaagagcag aaacaccggg agtgccagaa gcagatccct
      541 gggagcccag gacccgcttt ccatcatggt tgcagggtgt ctgatggtgt ttgtctggcc
      601 attctcgatc ttgatttccg tcttgggcgg tgtctcgtgt ttcaccagcg gaatgtactg
      661 gtgcacatgc atccgcaacc agccgttggt gatctcgaag atacggtttg aaaaatacat
      721 gctggctttg ggatattgct tgcgcagaaa gtggttgatg acccggctgg cgttggtggt
      781 ctctccccag gagagaggcg gatcaggaat cttggcaatg gcattggccc ggatcaggag
      841 tgtcatgtaa agcatcagcg gaagaaggaa aaagagcgcg cccttgaaca acagggtcag
      901 cgttgcggtg aactgctcgt cggcggtaca cttccttctc gactgcagcc aggtgtaaac
      961 gatgatggtc acaccgagaa gccagagaag cgtgacaaaa aggtcaaagc cccaacccac
     1021 cccgatgaaa tcgagcgact ccctgatgcg ggatccctgg aaggtggcgc cggggaggaa
     1081 aaagagcagg agtccgagga gactcatgat gaacttctcg gcgaccacca gcaggaacag
     1141 gaaggtccag ccaacgagag ccaagcccgt ccagagattg gcccgcgaag gggcggaagc
     1201 ccgcgcgagc gggagccggg agagcatcac gatggacgag acacccaaag cgatcaacgg
     1261 aagcaaaacg gcaatcaggg tccgcgccgc atgtttctcg tcgagaagac tggtcgtcca
     1321 ggcattcgac caaaacaggc cggcggcaat cagcgaggtg atcccgatcc cgatgcagac
     1381 cacgattttc gaggcatggt aggtctcctt gttggtggag accgttttca tggaagcgat
     1441 ccaggaaaca aaggccaccc ctgccggaat cagggaaagc acgacaagga gaacagcgaa
     1501 cacagccgac ggaagtccgt ccgcgaacaa tgccaacatc agtccgaaac ccagcgatgc
     1561 cgcggtacca atgtacacca ggggcgttaa tactttggta ttgcccgcgc cggaagcacc
     1621 accccggata tgctgggtta cgatcatgaa aaagaacacg agaggcggaa catacccgac
     1681 aagcagcagg tggtttgtaa aagacatccc caaaagcagc gcaataagca gaaaaagccg
     1741 ctcaccaatg gatggccggt cgttggcaat ggaggcatag acttcctcct gccagaccag
     1801 gaccgcaagc aggatcatgg gagcaaagat cgcgttcagg gtgtagacct ctgcgatcac
     1861 cgcctgggac cacatcgtcg acgaaaaggc aaacgccata gtcgcaccga aagagatgac
     1921 cctgtccctc aaggcaagac cagcctcggc cccccggtgc atcagacgca tgatcaggag
     1981 atacaggaac ggaatcgtca acgctccgaa aaagacagag aggagattca cccgaaaagc
     2041 cacactcccg gccggaacat aggtgaacac cttgccgagc atggtgtaga gcggataccc
     2101 cggtggatgt gccgctccca gattgtaaat tgcggttgtt agttcaccgc tgtcacccgc
     2161 acaaatctcg ggtgtcatcg tgagtgtata gacgacaaag gccaggagaa atgccgcccc
     2221 tgcaatcatc cagtcaatcc gttcgaatcg ccagatgggc agacgcatcc cgtgcttcag
     2281 cccgaaatcc gagtttccaa tgaggttcat gttcattccc ggtgtcttga ttggctcccc
     2341 tcttgcagag agcacagcaa gaataaagta gtccctgaag aggactccgg tcaaggcagc
     2401 cggatgtatt ggatgacgga aacgatggaa aacagaatac tctcgcaggc cagcacaggc
     2461 agatggatcc gggatggata gctgttttcg tttcgaacca gggtggaaag ctcactcctg
     2521 attctccact ggccgtcgga tctcgtcata gactgatcat gaaaaggacc ctttgaaagc
     2581 ggagagccga cagcattgcc ttcgtggtgc agctcaggtg aaaacgagcc cctgcatcat
     2641 tccataacca agcgcacagg agcatcgacg ttcgacaaga gagtgtacag ggctatttgt
     2701 ctctcggaga aagaaagagc ctgccggaat gcggcaggct ctatccgtcg cccttttttc
     2761 gtccgtgagg gcggccatct ggacggggcc cggaactggg gccagcgggg ctcgaacgtc
     2821 ccgccaaacg tgcagggtca ctgtactgtg aaccacccga ccctgatcac atccttattc
     2881 tataccagaa gggtccggat gtcaaggtcc ggcagaaaat ttttcatcct tggtgccgat
     2941 ccggtcggtg tttgtacaca atcatgcttt tgcactatga atcccggata cccatgacaa
     3001 tatcatgacc ctgtaatgag tctcgcctgt atcggtgtgc aacgaacact gtaccacaca
     3061 gctactcccg accgccagcg gtctcgtccg gtctggcatg tacctcccgc gaaaaccccg
     3121 actgcatccc gtccttcgtg taggccgtca caacgatata gtacaacctg tcgttttcca
     3181 gatttctcag ggtataggag ggattggccg gatcgagtct ggacaacggc acatcgatcg
     3241 gactgtccgg atgcaggtac tcaccccgct ccgtccccag atagatgcga tacccggcga
     3301 tgcgatccat attcgcccgc cagcggagga tgatcctccc gtccccggga cgtgcggtca
     3361 gttggaccgg cgtgtcggga cgccaggcgg gatcccagac gagccggacg ctcttcaaga
     3421 ccggcgtata ccggccccgt tcactcccca caaggatggc acgccactgg acaaaccgcc
     3481 cccgcggaat ctcgaaaccg gttgtcggat tccggagccg tatccaggga atttcgccat
     3541 cgtcggccgg gaagatttct cccgaaatcc ggtactccag aagaattgcc gagccggcag
     3601 gtgtctcgga cttccaggag gccgattcca ttttggcgtt gtccgtaccc atatcgacaa
     3661 cacgggatgt gatcctgccg atcgctgtac tgtaccggcc agtttccgaa tgcacccagg
     3721 cccctcgacg gatcctgaaa tcctctatcc gtccgcggaa gccccctccg agctgggcct
     3781 gacgccggaa tcccttgaga aaagccggcc tgaggggtgt ccctccctgg cgacctgtat
     3841 ccgtcagcca gacaatgccg tcctcgacac catcgacctg acgtgacaat tttccgctgg
     3901 catcatcata gataaacgca acatgctgcc agactctgag ccggctgaca cgctgtccga
     3961 cgagttccgc atcatgaacc cgctctcccc ggccatggag catgtttttc aaaaggaaga
     4021 cgagtctgtt gttcctgaaa aagatcctca ttcctgtttc aagaacacgg ccatccgccg
     4081 caagaaggtt gccataccgt gaaaaaacat cctcttcatc gtactgggaa tacggccaga
     4141 tccagaaatc aatggtaaaa ctgcccatat ccgtgacttc accgagcagg gctccggtgc
     4201 gaggtgtcaa tagaagtctg gtgtccggcc cgggaaaccg catgctcccc gcaccaaaga
     4261 gcgggaggat tgtatcaatg acaaccgaac tggaatcgat tccataattt ttcacccgga
     4321 aatcatcagg cttgttgccg tgaaggagga ggtccgtcga atcatcgtct tccgcctgat
     4381 tgtcggccag tctcaacgct tccgtcccgt cggtatcgtt gacaagacgg agattatcga
     4441 ggctggcacc gataaagcgg tctcccatgc cgctctccca gacccttgcc cctctcaacc
     4501 cggctgggat cagcaaacac accaggatca agagcgcgca aaatgacggc cagttcatac
     4561 gacatgactt cataagaaaa cccctcctca gatactttcg gcaaacccga cggattggtt
     4621 tatcgcttcc tttcctttgg aggaaagacc tcttcatggg aagccgctct tttttgccta
     4681 atcgacacga ctggtctata cttatattac agtgtctatg ctattcgtgg cggagtgcac
     4741 ataatggaag gtatccgggt tttaatggct gctgcggaag ccgcgccctt tgccgttttg
     4801 ggtgaactgg cccaggtcat gggctccctt ccgtactacc tcaaaaatct tggctgcaat
     4861 gtcagagtga ttctccccta ctatccctcg gtcgccaacg cttctccggt cacgccgatg
     4921 cccatcggag agatccgttt cacgatgaat ggccgcgagg aacgcgcaat catccgcgga
     4981 accgaacgtc atggtgtcct cttctacttt gtcgagcgtg atgacctcta tggccgcgag
     5041 gggctctatc agtaccggaa tgacgacgga acaatgcagg actacgagga caacctctac
     5101 cgttttgcct ttttttccag agccgtcctc gaggcctcgg ctgtaaccgg attctatccc
     5161 cggatcattc attgcaactc ctggcacact gcgctggttc ccgtctacct caagacgatc
     5221 tacgcccgat cctcggcaca cagcaagaca tcaaccctgc tcaccattcc aaatatggca
     5281 ttccagggac tcttcccctc ggctcagtac aatacaaccg gtcttccctg ggaagtcttc
     5341 aacacaaacg cccttgaata ctacgggatg atcaatct
//
LOCUS       DAOSHE010000101         5362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13567_length_5362_cov_4.086866, whole genome shotgun sequence.
ACCESSION   DAOSHE010000101 DAOSHE010000000
VERSION     DAOSHE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13567_length_5362_cov_4.086866"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLD82_04085"
     CDS             <1..316
                     /locus_tag="PLD82_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Appr-1-p processing protein"
                     /protein_id="HOG64598.1"
                     /translation="ELSLVPGALEDAGHYLETHIPVKEHIERVADLVQGFESSFGLEL
                     LATVHWVVTRQKITPPDEVVTQVHAWNARKKQFTPRQIRLALDTLMRKRWCEGALAVS
                     AF"
     gene            complement(337..546)
                     /locus_tag="PLD82_04090"
     CDS             complement(337..546)
                     /locus_tag="PLD82_04090"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOG64599.1"
                     /translation="MTTKQLEEEALTLKSIDRIHLVEKLLISLNRPDPKMEKEWVLES
                     EARYKAYKHGEIEPVSLQHIRNTHS"
     gene            737..988
                     /locus_tag="PLD82_04095"
     CDS             737..988
                     /locus_tag="PLD82_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HOG64600.1"
                     /translation="MSEPTNRLRNLAISDTGFVFDPSTGNTFLLNETGIFILRKLVQG
                     CARDAVVEALVEEYDVDAEQAGRDLADILIQMQEFGMAS"
     gene            985..2040
                     /locus_tag="PLD82_04100"
     CDS             985..2040
                     /locus_tag="PLD82_04100"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG64601.1"
                     /translation="MKPITIAVTGLNAVDSPGPGVPVLRSLLASSLECRLVGLAYDVL
                     EPGIYMPDLVDATFLLPYPNSGPAALIDRIRTIHAKVPLDFIIPTLDAELDNFILVKE
                     QLAEMGIKVFLPGKEQLHLRDKTRLAESLREKGIDVPETVIMGDLAGIRRAMETIQFP
                     FLIKGLFYEAYLASGYEEAVGYFSKVAARWGLPVIAQERIKGEEYNVIALANHGEVVG
                     AVPMKKMYLTDKGKAWAGVTIDNPELLENATKIIREIGWNSGCELEYIKEEKTGRLYL
                     LEINPRFPAWIWLATAAGQNLPEMLIRLANGETPAPVREYQYGKVFVRHSWDDIVDMS
                     EIAMLSGTGEFVRKVED"
     gene            2042..3388
                     /locus_tag="PLD82_04105"
     CDS             2042..3388
                     /locus_tag="PLD82_04105"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG64602.1"
                     /translation="MSGENTEKKIYVRPTIRRQFMGHANKFGGLAIPEPVVSIDGVAV
                     ADLVREFGSPLFVFSEATLRRKVRELNHAFSSRYPRFQAAWSYKTNYLDAICRVFHDE
                     GSWAEVVSGFEYKKARANGIPGNRILFNGPYKPLPVLREAIESGTRIHVDHLDELSDC
                     LTIARELGRPVSVTLRINMDTGTWPSWDRFGFNLESGQALEAVRRIAASDGKLRLTGL
                     HAHIGTFMMDSAPYKRAAAKLAQFWKKIVSEYAQPLEYLDLGGGFASANRLKGQYLSG
                     SVMLPSFDQYAEAITSALYEGFEPAEPPQLFLETGRALVDDAGFLVTSVVANKTTPDG
                     KRALVIDAGVNILFTAYWYDITAAPSTRHAGSWEEVALFGPLCMNIDKVRESCLLPSL
                     QRGEQIVLHPVGAYNVTQWLQFIEMRPNVVMIDRDGKPKIIRHAESLDDIVRREEV"
     gene            3388..>5362
                     /locus_tag="PLD82_04110"
     CDS             3388..>5362
                     /locus_tag="PLD82_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOG64603.1"
                     /translation="MRMPLVVEAGLSGYSAVLFSDSRILGAGILAATFVNPWTGLTGL
                     IGVLFSNVLAMLFGVHRERIAKGLYGFNGLLVGLSVSLHHQVDWGLIALLLVALVFLL
                     MLTLSLEHFLGYFFGLPPLSLPFVITSMILYFALYNYTAFTPNVPAVFPGDAFFPDLP
                     YHVLLFLKSMGAIFFQTSPWAGLVVALAVLAFSRLAMVLAIIGYAAGVLFHGLMGGNP
                     QDIAPGSIGFNNILTAVAIGGIFVVPGYRSFLLAFFAAMVSSLMGSFAKIFLIHFSMP
                     LLALPFTTVTLLFLHGLRTLGVGRFRLVDFAPGSPEKNIDYYTTRLKRFGESGLEIRL
                     PFHGQWKVTQGYNGEHTHKDLWRESLDFMALTPDGALWQGARASVNDYPTWGLPVLAP
                     ADGRVVRVVNHLDDNPLGQLDTKNNWGNLVLIQHAPFLYSQLSHLKKGSVTVHEGEMV
                     RSGARLAQAGNSGRSSEPHLHIHFQRTPEVGSPTLAVSFSQLVSHGERAEIRFNAIPQ
                     EGAAVSPVVADFTMSTFFGLAPGLELTVRMECAARDGGEKRSRLEAWQISLDFFGNRY
                     IENDAGDRLYYQAGDSWFACLGYRGGRESALFGTFLAVYRIPFAGMETGWQDRMSHSF
                     FHSLPVNIVYDMLLPFTDRFSMQWHGRIVTSDAG"
BASE COUNT         1098 a         1408 c         1543 g         1313 t
ORIGIN      
        1 ggagctctct cttgttcctg gtgcgcttga ggatgctgga cattacctgg aaacgcatat
       61 tccagtcaag gaacatattg agagggtcgc cgatcttgtc cagggctttg aatcttcctt
      121 cgggctggaa ctcctggcca cggtgcattg ggtagtcacc cgacaaaaaa ttactccacc
      181 tgacgaggtt gtcactcagg tccatgcctg gaatgcccgt aagaaacaat ttacgccaag
      241 acaaatccga ctcgccttgg atactctgat gaggaagcgg tggtgtgagg gtgcgcttgc
      301 ggtttctgct ttctgaggat tattgcattg tatttatcat gaatgcgtat ttcggatatg
      361 ctgcaatgag accggttcaa tttctccgtg cttgtaggct ttatatctgg cttcagattc
      421 cagaacccac tccttttcca ttttcggatc aggccggttg aggctgatga gcagtttttc
      481 aaccagatga atcctgtcta tggatttgag cgtcagggct tcttcttcaa gttgctttgt
      541 tgtcatgaga tcctctctct gtggggtatt gtacaatgga ttgagacgaa tttcaaacaa
      601 gaacaggctc gagtgtattg aggatcttgt tcgaggtcct caatacaccg gataaataca
      661 ataaaaaacc ctgaaaccct ggcaggaacc tggtatatag tggtgtatag taaaaatcct
      721 ataccaggag cagcctgtgt ccgaaccaac caatcgtttg cggaatttgg ccatcagtga
      781 taccggattt gtcttcgatc catctacggg aaacaccttt cttctcaatg agactggaat
      841 cttcattctt cgcaagctcg tgcagggatg tgcgagggat gccgttgtcg aagcgcttgt
      901 agaggagtat gatgtcgacg cggaacaggc cggtcgcgat ctggcggata ttttgatcca
      961 gatgcaggaa ttcgggatgg cctcatgaaa ccaatcacga ttgccgttac cggattgaat
     1021 gccgttgaca gcccgggtcc gggcgtgcct gtcctccgct ccctcctggc gtcctcgctg
     1081 gagtgccgcc tggtgggact ggcctatgac gtgcttgaac cggggatcta catgccagat
     1141 ctggtggatg ccacgtttct gcttccgtat cccaactcag gacctgcggc cctcatcgac
     1201 cggatccgga ccatccatgc caaagtcccg ctggatttca tcatccccac cctggatgcc
     1261 gagttggaca atttcatcct tgtcaaggaa caactcgctg aaatggggat caaggttttt
     1321 ttaccgggca aggaacagct ccatctcagg gataagaccc gactggccga gtccctcagg
     1381 gagaagggga tcgatgttcc cgaaacggtg atcatgggag acctggcggg gattcgccgg
     1441 gcgatggaaa ccatccagtt tccctttctg atcaagggac tcttttacga ggcctatctt
     1501 gcttcgggat acgaagaggc ggtcggctat ttttccaagg ttgccgcgcg ctggggattg
     1561 ccggtcattg cccaggaacg gatcaagggt gaggaataca atgtgatcgc cctggccaat
     1621 catggcgagg tagtcggggc tgttcccatg aaaaagatgt acctgacgga caagggcaag
     1681 gcctgggccg gggtgacgat cgacaatcct gaacttttgg agaatgccac aaaaatcatc
     1741 agggagatcg ggtggaatag cggatgtgaa cttgaataca tcaaggaaga aaagacagga
     1801 cggctctacc tcctcgagat caacccgagg tttcccgcct ggatctggct ggcaacagcg
     1861 gccggacaaa accttccgga gatgctcatc cggctggcaa acggcgagac accagctccc
     1921 gtcagagagt accagtacgg gaaggtcttt gttcgccaca gttgggatga catcgttgac
     1981 atgagtgaga ttgcaatgct ttcagggaca ggggaattcg ttcgaaaggt ggaagactga
     2041 tatgtctgga gaaaatactg aaaagaaaat atatgtgcgc ccgacgatcc gcaggcagtt
     2101 tatgggacat gccaataaat ttggcggcct ggccattcct gagccggtgg tcagtattga
     2161 cggcgtcgcg gtggcggatc ttgtgcgcga gtttggttca cccctctttg tgttctccga
     2221 ggcgaccctc cggcgcaagg tgcgtgaact gaatcacgcc ttttcatccc gctatccgcg
     2281 attccaggca gcctggtcct acaagaccaa ttatctggat gccatctgcc gtgtgtttca
     2341 tgatgaaggg agctgggccg aagtcgtttc agggtttgag tacaaaaaag cgcgggccaa
     2401 tggcataccg ggaaacagga ttctcttcaa tggcccgtat aaacccctgc ccgttttgcg
     2461 cgaagcgatc gagagcggga cgcggatcca tgtggatcac ctggatgaac tctcggattg
     2521 tctgacgatc gcccgtgaac tggggcgacc ggtttcagtc acgctgcgga tcaatatgga
     2581 cacgggaacc tggccttcat gggatcgctt cggctttaat ctcgagtccg ggcaggccct
     2641 tgaggcggtg cgcaggatag cagcgagtga cgggaagttg cggctgaccg ggctgcatgc
     2701 ccacattgga accttcatga tggattccgc accgtacaag cgggctgcgg caaaactcgc
     2761 ccagttctgg aagaagattg tctctgagta tgcacaaccc ctcgagtatc tcgatctggg
     2821 tggcgggttt gcctcggcca atcgcctcaa gggacagtat ctctccggat ccgtcatgct
     2881 cccatcgttt gaccagtacg ccgaggcgat cacctccgca ctctatgaag gctttgaacc
     2941 ggccgagccg ccccagctct ttttggagac tgggcgggcc ctggtggacg atgccggctt
     3001 cctcgtgacc tcggttgtgg caaacaaaac cacgccggac gggaagcggg ctctggtaat
     3061 tgatgccgga gtcaatatcc tctttaccgc ctactggtac gacatcacag cggcaccctc
     3121 cacccggcat gccggctcct gggaagaggt cgctctcttt ggaccgctct gcatgaatat
     3181 cgacaaggtc cgggagagtt gtctgcttcc cagtctgcag cgaggagaac agatcgtcct
     3241 gcacccggtc ggggcctata acgtcacgca atggctccag ttcatcgaga tgcggcccaa
     3301 tgtggtcatg attgaccgcg acgggaaacc aaagatcatc aggcatgccg agtctcttga
     3361 cgatatcgtc aggcgggagg aagtctgatg cggatgcctc ttgtcgttga ggccggcctt
     3421 tcggggtatt cggctgtcct cttcagtgac agccggattc ttggggcggg cattcttgcc
     3481 gctacctttg tcaatccctg gaccggattg accggtctga tcggtgtgct tttctccaat
     3541 gttctcgcga tgctctttgg agtacatcgc gagcggattg ccaaggggct ctatggcttc
     3601 aatggtctat tggttggact ctcggtcagt ctccatcacc aggtggattg gggcttgatc
     3661 gccctgctgc ttgtggctct tgtgttttta ctcatgctga cgctttcgct cgagcatttt
     3721 ttggggtatt ttttcggatt gccgccgctt tccctgccct ttgtgatcac cagcatgatc
     3781 ctctattttg ccctgtataa ttacacagcg ttcacaccca atgtgcctgc cgtctttccc
     3841 ggtgatgcct tttttcctga tctgccatac cacgtgctgc tttttcttaa aagtatgggg
     3901 gcgatctttt ttcaaaccag tccctgggcc gggcttgtgg tggcgctggc cgttcttgcc
     3961 tttagccggc tcgccatggt cctggcaatc atcgggtatg ccgcgggtgt cctgtttcat
     4021 ggactgatgg gagggaaccc gcaggacatc gcaccgggtt cgatcgggtt caacaacatc
     4081 ctgacggccg tcgccatcgg gggcatcttt gtcgttcccg gatacaggag ttttctgctg
     4141 gcattttttg cagccatggt cagttccctg atgggatcct ttgcaaaaat tttcctgatc
     4201 cacttcagca tgccactttt ggcactcccg ttcacaaccg tgacgctgct cttcctgcac
     4261 ggattgcgta ccttgggagt gggacggttc cgtctggtcg atttcgcccc gggttcgccc
     4321 gaaaagaaca tcgattatta cacgacacgg ctcaagcggt ttggagagtc cggactggag
     4381 atccggctcc cgtttcacgg gcaatggaag gtcacgcagg gctataacgg agaacatacg
     4441 cacaaggacc tctggcggga gagtcttgat ttcatggcgt tgacgccgga tggcgccctc
     4501 tggcagggag cacgagcgtc cgtcaacgat tatccgacct ggggactccc ggtgctggca
     4561 ccggccgatg gccgggtggt gcgggttgtc aatcatctgg atgacaatcc gcttgggcag
     4621 ttggatacaa aaaacaactg gggaaatctg gtcctcatcc agcatgcacc gtttttgtat
     4681 tcacaactct cccatctcaa aaaagggagt gtgaccgtgc atgaaggcga gatggtccgc
     4741 tccggggcca ggctggcgca ggccgggaac tcgggccgga gcagtgagcc acacctgcat
     4801 atccacttcc agcggacacc cgaggttggg agcccaacac tggccgtttc gttttcgcaa
     4861 ctcgtgtccc atggagagcg tgcagagatc cgcttcaatg ccattccaca ggagggtgct
     4921 gcggtctcac cggttgtggc cgatttcacc atgagcacgt tttttggatt ggctcccggt
     4981 cttgaattga ccgtgcggat ggagtgtgct gcgcgggatg gcggtgagaa gcgcagccgc
     5041 ctcgaggcct ggcagatctc cctcgacttt ttcggaaacc ggtacatcga aaatgatgcg
     5101 ggcgacagac tctactatca ggcaggagat tcctggtttg cctgcctcgg gtacaggggt
     5161 gggcgcgaga gtgcattgtt tggaaccttc cttgcggtgt acaggattcc gtttgcggga
     5221 atggagaccg gctggcagga cagaatgtcg cacagctttt tccattcgtt gcccgttaat
     5281 atcgtctacg acatgctctt gcccttcact gaccggttct cgatgcagtg gcacgggcgg
     5341 atcgtcacaa gcgatgccgg ac
//
LOCUS       DAOSHE010000102         5321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_13725_length_5321_cov_4.857387, whole genome shotgun sequence.
ACCESSION   DAOSHE010000102 DAOSHE010000000
VERSION     DAOSHE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13725_length_5321_cov_4.857387"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1256)
                     /locus_tag="PLD82_04115"
     CDS             complement(135..1256)
                     /locus_tag="PLD82_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64604.1"
                     /translation="MQPMRVITCVVLITAILVTPCALANSKPSPDDLARFLAGLEAPA
                     GSGLETMTATAWYQRHARLATSAWKRYRETTLLPLRDWALRQRLPQLGTLVYPFAGPD
                     LVNALTLYPDVPEYLLFGLEDPGSLPDPATLVPGDLADLRSALATFFAANHFFTREME
                     RRMGGPSTSVSAVFLCFLASSEYKVLDFKRIGLAEDGEVVDGSGTDGAAGRDTGRIHG
                     IEIRFSGNRGLAKTLRYFKVDVSDQSLKSRGAGFLRLLARCGRFATLMKAASYLMHRE
                     VRTDLPPHFSFLRDVLLSRSETVLQDDSGIPLRFFSRLLWNRRFFGKYTQPVPEFVKR
                     MQPDLWNEMCYTSRGSLPFFFGYDRRPGAANIMVLERIR"
     gene            1601..2872
                     /locus_tag="PLD82_04120"
     CDS             1601..2872
                     /locus_tag="PLD82_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64605.1"
                     /translation="MRDLTILSILLLLVLSVRCGSGKDPYFSKDFATGNQISHRSPNS
                     TEKSDRAALLYKAESLLKDGKAFEAVTVFKTVLDAGTDAWTYFRYGDALANAGRYKDA
                     ISAYDCSLVLLPTNRAIVYYNKACAASLGKDRDISFQALKESLIEGYTNFEWMYQDPD
                     IQWLFTQFTGTSSLTNEIEALRPFQTLKDLREVDPKIFCYSWIRTPWLKRMQSGHGVI
                     AGHDRGELIIGLQNANGSTNTNGSTYVVMKTDDYHQMTFYGKLKRCLFQQTTGRYFLD
                     TEQKPLTVVSFNGNSLILRDRDRLLDYTKMPVFLDKQGISRPLIWNLVLAGQYTDNHG
                     HTWDFSPDSRISIDHGERKPFRIRLDPYGSLNIDMFISVDTNYLMYKGMKIDYCGFRK
                     ITNGLDVMFYHWSKDSLPVLSETIRLTKIRK"
     gene            complement(3007..3660)
                     /locus_tag="PLD82_04125"
     CDS             complement(3007..3660)
                     /locus_tag="PLD82_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG64606.1"
                     /translation="MTRPRVKICCISSIVEAQAAIRSGAAAIGLVGQMPSGPGIISDD
                     LICTIAQAVPPPIATFLLTSETTAAGIIAHYQKAHTSTIQLVDEVESSQYALIRAALP
                     GVKLVQVIHVIDKGSVQQAKDISCHVDAILLDSGNPKLAVKELGGTGRTHNWEISRDI
                     VRAVSVPVFLAGGLNSGNVRQAVQTVQPFGIDLCSSVRSDGHLDERKLKELFEALGG"
     gene            complement(3726..>5321)
                     /locus_tag="PLD82_04130"
     CDS             complement(3726..>5321)
                     /locus_tag="PLD82_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOG64607.1"
                     /translation="GQGATCLEISVGGEMAETGRTANPLVGIAERPGFVRIVHAIRRA
                     ALDERVRAVIVHLRANGLGWARSEELMQALLVFRSKKIPVIASIEYADLKALLIASAA
                     DEIHIHPSAQVRTAGPGMVVTLYTGLLEKLGLQARLLYRSEYKTAAQTVTEKSMTPAH
                     RQSAERVLEVIRNRAIRVIAANRVVPVTRVEEWVAAGLVTAKDAVARGMAAGNMSWDD
                     LRRTIAGKGFNLAGPAAWLEPERGFTQNRGIAIVQVEGTIVNGAGGLPQLPFGEMAAG
                     ADSVLGAVSRALADPAIRGILVRVQSGGGDVLASERMRAALAQAAARKPLAVSMGDVA
                     ASGGYYLACGDGRTRIPIFAKAATLTGSIGVITGKLVTSGLRANLGVKTTRLGDTNVH
                     LYGDEEDFTPAQVQLVTKVLDYWYDEFKNSVARGRGLTPAQVENHARGRVWAGSDALE
                     RGLVDHEGGAFEALLSISARAGLTLSETPLYEMNSRVAGLASLVTGGINLQMEGLLDA
                     ESWPGLFFSGRALALHPWSLRVE"
BASE COUNT         1234 a         1551 c         1369 g         1167 t
ORIGIN      
        1 ataaagatgt ttttggcggc cggtgtcacg gcgatctcgc cctttttttc aaccgaagat
       61 caaccctcca acacacagac aacaagcagt cccaacacac cggtctttgc cataccgtct
      121 ctcccgaacg gcccttaacg gatccgttcc aaaaccatga tattagccgc acccggcctg
      181 cggtcatagc cgaaaaagaa cggaagcgaa ccccgtgaag tatagcacat ctcgttccac
      241 aaatccggct gcattctttt gacaaattcc gggacgggtt gcgtgtattt cccgaaaaac
      301 cggcgattcc acagcaggcg ggaaaagaag cgcaggggga tccccgagtc atcctgcaaa
      361 acagtctcgc tccgcgagag gagaacatca cgcaggaagg agaaatgggg ggggagatcg
      421 gtgcggacct cccggtgcat gagatacgaa gccgctttca tcagggttgc gaatcgcccg
      481 caccgcgcaa gaaggcgcaa aaaaccggca cccctgcttt tcagactctg gtccgaaaca
      541 tcgactttga aatagcgcag cgttttggcc agccccctgt tccccgaaaa tcgtatttca
      601 atcccatgga tacgtccagt atcgcgccca gccgctccat cagtgccgga tccatctaca
      661 acctctccat cctccgcaag accgatcctt ttgaaatcga ggactttgta ttcagatgac
      721 gcgaggaagc agaggaagac tgcgctgacg ctggttgacg gcccgcccat gcgccgctcc
      781 atctctcggg taaaaaaatg gttggccgca aaaaacgtgg caagagctga ccgaagatcg
      841 gccagatctc ctggcaccag cgtggctgga tcgggaagcg agcctgggtc ctccaggccg
      901 aagaggaggt actccggaac atcaggatag agtgtcagtg cattcaccag gtccggtccg
      961 gcaaaaggat agaccagtgt cccgagttgg ggcagacgct gtcgaagggc ccaatcccga
     1021 agcggcagga gtgttgtctc gcggtagcgt ttccatgcgg aggttgccaa gcgggcatgc
     1081 cgctgatacc aggctgttgc cgtcatcgtc tccagcccgg agccggcagg ggcctccagc
     1141 ccggcaagaa aacgcgccag atcgtcaggc gagggtttcg aattcgccag cgcgcaggga
     1201 gtaacaagaa tagctgtgat caggaccacg caggttatca ctcgcatcgg ctgcatacgt
     1261 cagcctatgc ttgaagagaa ggtttgtcaa ggaaaagact ccgtgatgaa ggttggtcga
     1321 tcagatgcac agtaaaaata ttctcgctga tctcaagcat gatctcgcac agggctggaa
     1381 agtttttgca gcaagtataa atcttttact taaatttttg aagatgatag cccacagctt
     1441 tcatctctcc attcttaaca cgaatttttt tggtgcctcg cgatcgctga aatcattcta
     1501 ttcgatattg ctttttatat tgtatcatga catcagaatc aatttgaccg tataaccgga
     1561 acatgctatt gtgatcttga ttgctttttg gagaatgata gtgcgtgatc tcacaattct
     1621 gtcgattctg ctcctgcttg tcctttctgt ccgatgtgga tccggaaaag atccatactt
     1681 ttcaaaagat ttcgccacag gaaatcaaat ttcgcaccgc agtccgaact ccaccgaaaa
     1741 atctgaccgg gctgccctgt tatacaaagc cgaatcactt cttaaggacg ggaaagcatt
     1801 cgaagcagta acggtattca agacagttct tgatgcaggg actgacgcct ggacctattt
     1861 tcggtacggt gatgcccttg ctaatgcagg tcggtacaaa gatgcgatat cggcctatga
     1921 ttgttccctc gtactgttgc cgaccaatcg agccatcgtc tattacaaca aagcctgtgc
     1981 cgccagtctg gggaaagata gagatatcag tttccaagca ctgaaagaat ctctcatcga
     2041 aggatatacc aacttcgaat ggatgtatca ggatcccgat atacaatggc tctttactca
     2101 gttcaccggc acttcatcac tgaccaatga gatcgaagca ctccgtccat ttcaaaccct
     2161 caaggatctt cgtgaggtcg atccaaaaat cttttgctat tcctggatcc ggacaccttg
     2221 gctcaaacgg atgcagtctg gacatggagt catagccggg catgacaggg gagagttgat
     2281 cattggactc caaaacgcga atgggtccac aaatacgaat ggatcaacgt acgtcgtgat
     2341 gaaaactgat gattatcatc aaatgacttt ttatggaaag cttaaacgct gcttgtttca
     2401 gcaaacaacc ggtcggtatt ttctcgatac agaacaaaag cccttgacag tcgtttcatt
     2461 caacggtaat tcattgattc tccgagatag agatcgactt ttggattata caaagatgcc
     2521 cgtatttctt gacaaacagg gaatatcgcg cccactcatc tggaatcttg tacttgcagg
     2581 gcaatatacg gacaaccatg gtcatacttg ggatttctca cccgattcac gaatcagcat
     2641 tgaccatgga gaacgcaaac ctttcagaat ccgtcttgat ccgtacggat ctctaaacat
     2701 tgatatgttt atttctgttg atacaaacta tcttatgtat aagggtatga agatcgatta
     2761 ttgcggcttt cgaaaaatta ccaacggatt ggacgtcatg ttttatcact ggtcaaaaga
     2821 cagtctcccg gttttatctg aaactatccg tttgacaaaa atccgcaaat aaaactctca
     2881 ttctgatcca tcccgtccct atacataaaa ggattggctt tctgctcact cgtgatcgtt
     2941 tcaagcccgt agtacctttt attctcgact cataccatat tgaatcaatc atgtgtatgt
     3001 gcccccctac ccgcccagag cctcaaacaa ttccttcagc tttcgttcgt ccagatggcc
     3061 gtcgcttcgg acactgctgc agaggtcaat cccaaagggc tggacggttt gcaccgcctg
     3121 ccgcacattg ccgctgttga ggccaccggc cagaaaaacg gggacggata cagccctgac
     3181 aatgtcacgg ctgatctccc agttatgcgt ccggcctgtc ccgcctaatt ccttgacagc
     3241 gagtttggga ttcccgctgt caagcaggat cgcatcaaca tggcaggaaa tatctttggc
     3301 ctgctgcacg gaacctttat cgataacatg aatcacctga accagtttga cgccgggcaa
     3361 ggcagcacgg atcagcgcgt actggctgct ctcaacttca tccacaagct ggatggttga
     3421 ggtatgggct ttttggtagt gggcaatgat gcctgctgcc gtcgtctcgc tcgtcagcaa
     3481 aaaggtggcg atcggcgggg gcacagcctg cgcaatggta caaatgaggt catcgctgat
     3541 aatgcctggc ccgctgggca tctggccgac caatccgatc gcagcagccc cagagcggat
     3601 ggcggcttgc gcctcaacga tactgctgat gcaacagatc ttgacccgcg gtcgtgtcat
     3661 tggcttctcc ccagtcattg cacagcaatc cgatgatctt caatgcttca cagccccacg
     3721 catccctatt cgaccctgag cgaccaggga tgaagggcca gggcccggcc ggagaagaag
     3781 agtccaggcc aggactcggc atccagcagg ccctccattt gcaggttgat cccgcctgtt
     3841 accaggctgg ccagtccggc cacccgtgaa ttcatttcat acaagggggt ctcgctcagg
     3901 gtcaaaccgg cgcgggcgct gatcgagagc aatgcctcga aggcgccgcc ctcatgatcg
     3961 acaagccccc tttcgagggc atccgagcca gcccagaccc gaccacgggc atggttctcc
     4021 acctgggcgg gcgtcagtcc ccgcccgcgc gccaccgagt tcttgaactc atcgtaccaa
     4081 tagtccaaaa ccttggtcac aagctgcacc tgggccggtg tgaaatcctc ctcatccccg
     4141 tagagatgca cattggtgtc cccgagtctg gtcgtcttca ccccgagatt ggctcgcaaa
     4201 ccgctcgtca cgagtttccc ggtaatcaca ccgatcgagc ctgtcagcgt ggcagccttg
     4261 gcaaaaatcg gaatccgtgt ccttccgtct ccgcaggcca gatagtatcc gcccgaagcc
     4321 gccacgtctc ccatggagac cgcaagcggt ttgcgggccg cggcctgcgc cagggcggcc
     4381 cgcatccgtt cgctggcaag cacatccccg cccccggact ggacccggac gagaatcccc
     4441 cggatggcgg ggtcggcaag ggcccgcgag accgccccga ggaccgagtc cgcaccggca
     4501 gccatctccc cgaacggaag ctgcgggagc ccgccggcac cattcacgat cgttccttcg
     4561 acctggacga tggcgatccc cctgttttgg gtaaagcccc gctccggttc gagccaggcc
     4621 gccggcccgg ccagattgaa gcccttgccg gcaatcgtgc gccggagatc gtcccacgac
     4681 atgttcccgg cggccatccc ccgtgccacg gcgtccttcg ccgtcacaag tccggcagct
     4741 acccattcct cgacccgcgt gaccggaacg acccggtttg cggcaatcac ccggatggcc
     4801 cggttgcgga tgacctcaag cacccgttcg gctgattgtc ggtgggctgg ggtcatgctc
     4861 ttttcggtga cagtctgtgc agccgtcttg tattcgctcc ggtagaggag gcgggcctgc
     4921 aagccgagtt tttccagcaa cccggtatac agagtcacca ccatccccgg tccggccgtc
     4981 cggacctggg ctgatggatg gatatggatt tcgtcggccg cagaggcaat caggagggcc
     5041 ttgagatcgg cgtattcgat actggcgatg accgggatct ttttggaccg gaaaacaagc
     5101 agggcctgca tcaattcctc gctgcgggcc cagcccaggc cattggccct gaggtgcaca
     5161 atcacggccc ggacacgctc atccagcgcg gcccgccgga tggcatgcac gatccggaca
     5221 aatccgggcc gctccgcgat cccgaccagg ggattcgcgg tcctgcccgt ttcggccatc
     5281 tccccgccca cactgatctc gagacaggtc gcaccctgcc c
//
LOCUS       DAOSHE010000103         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14127_length_5213_cov_4.680496, whole genome shotgun sequence.
ACCESSION   DAOSHE010000103 DAOSHE010000000
VERSION     DAOSHE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14127_length_5213_cov_4.680496"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLD82_04135"
     CDS             <1..444
                     /locus_tag="PLD82_04135"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG64608.1"
                     /translation="TDGSCGLAFPDKAPVYKKLHAIVTQSNEMPASFARIPDSFVPDL
                     ASGKYAGVTVHPTQLYESFSMLVCFALVMLLYKKKRFNGEAFLWVLGFYAVVRFLIET
                     VRIDTPHNLFLGTFSLSQSISLIGFPIIVAAIIIGRARAQRHIKS"
     gene            419..1369
                     /locus_tag="PLD82_04140"
     CDS             419..1369
                     /locus_tag="PLD82_04140"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG64609.1"
                     /translation="MHNDTSRVEDEDDGLVRLDLVIPPEMDGWRADQALTVLLGADWS
                     RSRIKRDMQSLTLDSRPVKLSERVVAGARLQPVLTDEPGFADLKPEAIPLDIVYEDAE
                     IIVINKPWGMAVHPSPGTPNGTVVNALLAHCKSLPGTDSFRPGIVHRIDKDTSGLLVC
                     VKTERARIALAEDFQERRVRKQYSAIVLGRMSPRQDVIDAPLGRDPVNRIKQAVVPGG
                     REARTGYHVVEEFSNHSLLGIDLFTGRTHQIRVHLAWKGRPIVGDPLYARSDTLSDRL
                     CLAAVRLGFEHPVSRHELEWTIPLPGHMQTALERLRSREG"
     gene            1406..1783
                     /locus_tag="PLD82_04145"
     CDS             1406..1783
                     /locus_tag="PLD82_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64610.1"
                     /translation="MTLVHAILIIAVPVLIGGIVIWLVFRNINRKYCDLFRKLVLAAR
                     ENPRKMQELVLSTNNRDRTRIVDEIAAIEEVEKVDIIKAGKKRSQLRLQAKSRGQARS
                     WHIVLASDAEKWGIVEFAEIDQG"
     gene            complement(1780..3357)
                     /locus_tag="PLD82_04150"
     CDS             complement(1780..3357)
                     /locus_tag="PLD82_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64611.1"
                     /translation="MAQPRILIVDDDELAGMSLRGHIKRMGYHECGMAANSGDALDLI
                     TRTAPDLVLMDIMIEGELDGVETAKLILQRFDVPIVYLTASADTETVERAKATSPFGY
                     LIKPIEENALRVTIEMALYKHAVENRIKRSQRHLEEINAAFLAFSDEPMDNITVVCEL
                     VSSILRSSCTMFLQLKTDGMSRPVCSTRTDGAQCRHICEKRADWIENLLSCVGDGITI
                     LPAGELGPPWKRVIVFPLKRIRRAPTVILTLQQHDDEISDEDMRLAGILVNAAKAQED
                     RIETLEARARMHQQLAQTQRLESIGTMARGVAHEINNPLMGIINLAQLVYDRSEGLPG
                     TQAWASEIISEGQRIARIVRSLLAFSQQDGSGQALVDIKEIIDDVITLVRRQLEKDEI
                     VLELEIAAHIPVFPCRRQQIEQVILNLVNNARDALLARFQTWNGEKIIKIRCLYEGQQ
                     IVLEVEDHGIGIPPEMHERIFDPFFTTKPRHKASGLGLSVSYGIIKEHGGDLSFESVP
                     GEKTVFRVTLPGRNETG"
     gene            complement(3456..4190)
                     /locus_tag="PLD82_04155"
     CDS             complement(3456..4190)
                     /locus_tag="PLD82_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOG64612.1"
                     /translation="MNGTVWEVLPDAILLPGLRSDGFVFAVSTVAAGDMHIATGDSGR
                     AARESFVRTYGMGLPLVCGNQIHGNRIEILTETKRAIFEGTDGCLVPQGVSVAAGVFT
                     ADCLAICLLDRRRREFVLVHSGWRGTQAGLPLKALRLLESRGTRMADLRVVFAPSIQP
                     CCYEVGPEFREHFPDGPFREQHGQLFFDNQGAAAGMLLSAGVPVSQIDVSPWCTVCDS
                     ERRFWSWRRQGQTAGRMMTIVAPIET"
     gene            complement(4200..4874)
                     /locus_tag="PLD82_04160"
     CDS             complement(4200..4874)
                     /locus_tag="PLD82_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG64613.1"
                     /translation="MITKILNVTKSYGSGSARAEALKGVSLDINAGDYIAIVGPSGSG
                     KTTLLSIIGCLIHPTEGEVYFDKRRVSDISDIELSVLRGREIGFVFQFTDLLSNLTVL
                     ENVLVPILFHDGMVDEKKKYAVQLLEKLGLGYCINARVQALSGGERQRVAIARSLINR
                     PRLLLADEPTGDLDDETSRRIISLFAAVNASGTTIVFVTHNKNFAKAATNVYEMGDGR
                     IQRILK"
     gene            complement(4898..>5213)
                     /locus_tag="PLD82_04165"
     CDS             complement(4898..>5213)
                     /locus_tag="PLD82_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64614.1"
                     /translation="GSLSRDRSSARDWRPAALCLKLLLVETWFIVLSGAVLSVILGFI
                     VVQARGEVLAPFFATAGFGSLIASVGAALGSTLVAATLAALYPAIVICSVEPYTAIRN
                     RE"
BASE COUNT         1141 a         1543 c         1442 g         1087 t
ORIGIN      
        1 accgacggaa gctgcgggct ggcttttcct gataaagccc cggtctacaa aaaactgcat
       61 gcgatcgtca cccagtcgaa cgagatgccg gcgagcttcg cacggattcc ggattccttt
      121 gtcccggatc tggcctcggg caaatacgcc ggtgtgacgg ttcatccgac acaactgtat
      181 gaatccttct ccatgctcgt atgctttgct ctggtcatgc tcctgtacaa aaaaaagaga
      241 ttcaacgggg aagcctttct ctgggtcttg gggttttacg cagtcgtccg tttcctgatt
      301 gaaacggtcc ggatcgatac cccccacaat ctttttttgg gaacattctc cctctcacaa
      361 tccatcagtc tgatcggatt ccccattatt gttgcagcca tcatcatcgg acgcgcccgt
      421 gcacaacgac acatcaagag ttgaggatga agatgacggc ctcgtccgcc tggacttggt
      481 cattcctccc gaaatggacg gatggcgcgc tgaccaggct ctgaccgtcc ttttgggtgc
      541 ggactggtcg cgttcgcgca tcaagcggga tatgcagtcg ttgacgctgg actcccgccc
      601 ggtcaaactc tctgaacggg tcgtggcagg agcccgcctg cagccggtct tgactgatga
      661 acccgggttt gccgatctca agccggaggc aattcctctg gatatcgtgt acgaggatgc
      721 cgagatcatc gtcatcaaca agccctgggg aatggctgtc catccctcac cgggaacgcc
      781 gaacggaacc gtagtgaatg cccttctggc ccactgcaaa tcccttcccg gaacggattc
      841 gttccggccg gggatcgttc acagaatcga caaggacacg agcggacttt tggtctgcgt
      901 caagaccgaa cgggcccgga ttgcgctggc cgaagatttc caggaacgtc gtgtccgtaa
      961 gcagtattcc gccattgtcc tcggaagaat gtccccacgg caggacgtca ttgacgcccc
     1021 tcttggcagg gatccggtca accggatcaa gcaggccgtg gttcccggtg gtcgcgaggc
     1081 acgcacgggc taccacgtcg tcgaggaatt ttcgaaccac tccctcctcg gcatcgatct
     1141 ctttaccggt cgcacgcacc agatccgggt tcatctggca tggaagggac gaccgatcgt
     1201 cggagatccg ctctatgcac ggagtgatac cctctcggac agactctgtc tggcggcggt
     1261 ccgtctcgga ttcgagcacc ccgtcagccg acacgaactc gaatggacga ttcccctccc
     1321 cggccatatg cagaccgctc tcgaacgcct gcgcagcaga gagggataaa cacttccagg
     1381 gtgcaccatc aaaaggagcc tgaccatgac ccttgttcat gccattctca tcatcgccgt
     1441 accggttctc atcgggggaa tcgttatctg gctggtcttc cgcaacatca accggaaata
     1501 ctgtgacctt ttcaggaaac tcgtcctggc cgcccgcgag aatccacgaa aaatgcagga
     1561 attggtcctc tcgacaaaca acagggaccg gacccggatt gtggacgaga tcgccgcgat
     1621 cgaagaggtc gaaaaagtcg acatcatcaa ggccggcaag aaacgttcac aactcagact
     1681 ccaggcgaag agccggggac aggcccgttc ctggcatatc gtcctcgcaa gtgatgccga
     1741 gaaatggggc atcgtcgagt ttgccgagat cgaccaaggt taaccggttt cattccggcc
     1801 cggcagggtc accctgaaga ccgttttctc tcccggcaca ctctcgaacg agaggtctcc
     1861 gccatgttct ttaatgatcc cgtaactgac tgacaggccc aaaccgctgg ccttgtgcct
     1921 gggctttgtc gtaaagaacg ggtcgaaaat cctctcatgc atctcgggag gaatcccgat
     1981 tccatggtct tcaacttcca gaacgatctg ctgcccttcg tacaaacaac ggatcttgat
     2041 gatcttttca ccgttccagg tctggaatcg ggcaagtaaa gcatcacggg cgttgttgac
     2101 aagattgagg atgacctgct cgatctgctg tctgcgacag gggaaaaccg ggatatgggc
     2161 cgcaatctcc aactcgagaa caatctcgtc cttttcgagc tgtcgccgga ccagggtgat
     2221 gacatcatca atgatttcct tgatgtcgac aagagcctga cctgaaccat cctgttgtga
     2281 gaaagccagg aggctgcgca caatccgggc tatccgctgg ccctcactga tgatctccga
     2341 cgcccaagcc tgtgtcccgg gaagtccctc actccggtca tagacgagtt gcgcgagatt
     2401 gatgatcccc atcaaggggt tgttgatctc gtgggccacc ccgcgggcca tggtcccgat
     2461 ggattcgagg cgctgggtct gggcaagctg ttgatgcatc ctggccctcg cctcaagcgt
     2521 ttcgatcctg tcttcctggg ccttggccgc attgaccagg atcccggcca gtcgcatatc
     2581 ctcatcgctg atctcgtcat catgctgctg gagagtcaga atcactgtcg gggcacgccg
     2641 gatccgcttg agcggaaaga caatcactct tttccatggt ggaccgagtt ccccggccgg
     2701 caggatggta atcccgtccc cgacacagga aaggaggttt tcgatccaat ctgcccgttt
     2761 ttcacagata tgccggcatt gcgcgccgtc agtccttgtc gagcagacgg ggcgactcat
     2821 gccatccgtt ttgagctgga gaaacatggt gcaactgctt cggagaatcg aagacacgag
     2881 ttcacagaca accgtaatgt tgtccatcgg ttcatcactg aacgcgagaa aagcggcatt
     2941 gatctcctca agatgccgtt gtgaacgctt gatccggttt tcgacagcgt gtttatacaa
     3001 cgccatctcg attgtcacac ggagtgcgtt ttcttcaatc ggtttgatca gatatccgaa
     3061 agggcttgtc gccttggccc gttcgacagt ctcggtatcg gcactggccg tcaggtagac
     3121 gatcgggaca tcgaagcgct ggagaatcag cttggcggtc tccaccccgt cgagttcgcc
     3181 ttcaatcatg atgtccatca ggacaagatc gggcgcggtc ctggtgatca gatccagtgc
     3241 gtccccgctg tttgcagcca tcccgcactc atgatagccc atgcgcttga tatgcccgcg
     3301 caagctcatt ccggcgagtt cgtcatcatc gacaatcaaa atccgtggtt gtgccatcag
     3361 ccctccccag atgatgatag cacatcttcg gcccttctcc aagacgggaa aaatcgggat
     3421 tggggaaaaa gttgtcagga tcggagtgcg atatctcagg tttcgatagg agcgacaatt
     3481 gtcatcatcc tgccggctgt ctgtccctgc cgccgccagg accagaaccg gcgttcggaa
     3541 tcacagacag tgcaccaggg ggatacgtcg atttgcgaaa ccggcacacc ggcggacagg
     3601 agcattccgg ccgctgcgcc ctgattatcg aagaagagtt gcccatgctg ttcgcgaaat
     3661 ggtccatcgg gaaaatgctc gcggaactcg ggaccgacct cgtagcagca aggctggatc
     3721 gagggggcaa aaaccacccg gagatccgcc atccgtgttc cccgggactc aagcagacgg
     3781 agtgccttga gcggaagtcc ggcctgcgtc ccgcgccaac cggaatggac caaaacaaat
     3841 tcgcggcgtc tccggtcaag gaggcagatg gccaggcaat cagccgtaaa caccccggcc
     3901 gcgacagaca ccccctgcgg aacgagacag ccgtccgttc cttcaaagat ggcgcgcttt
     3961 gtttcagtca gaatttcaat ccggttccca tggatctggt ttccacagac aaggggcaag
     4021 cccatcccgt aggtacggac gaaggactca cgggcggcac gaccgctgtc cccggtggca
     4081 atatgcatgt cacccgcagc caccgtcgag acggcaaaga caaaaccgtc ggacctcagc
     4141 ccggggagaa gaatggcgtc cgggagaacc tcccacacgg ttccgttcat caggttgtcc
     4201 tatttgagaa tgcgctgaat ccgcccgtca cccatctcgt agacattggt cgcggccttg
     4261 gcaaaattct tgttgtgcgt cacaaaaacg atggtcgtcc cgctggcgtt gaccgcggca
     4321 aagagcgaga tgatccgacg tgatgtctcg tcatccagat cccccgtcgg ctcatccgcc
     4381 agaaggagcc gtggccggtt gatcagggac cgggcgatgg ccacccgctg ccgttctcca
     4441 ccggagagtg cctggacccg ggcattgatg caatatccga gacccagctt ttcgagaagc
     4501 tggacagcgt attttttctt ttcatcaacc attccgtcat ggaagagaat ggggacaagg
     4561 acgttttcga ggacggtcag attcgagagc aggtcggtaa actggaaaac gaagccgatc
     4621 tcccttcccc gaagaaccga gagttcaatg tccgagatat cactgacccg ccgcttgtcg
     4681 aaatagacct ctccctcggt cggatgaatc aggcatccga tgatcgagag gagtgtcgtc
     4741 tttccgctgc ccgaaggccc gacgatggcg atgtaatcgc ctgcattgat atcgagcgaa
     4801 acgcccttca aggcctcggc ccgggcactc ccgcttccat acgattttgt cacattgaga
     4861 atcttggtta tcatcccgga ttgtccctct cgtcgtctta ctcgcggttt cggatggctg
     4921 tgtagggttc gacactgcag atcacgatcg ccggatagag tgcggcgagt gtggccgcaa
     4981 ccagcgtcga gccgagtgcc gccccgacgg atgcgattag cgagccgaac ccggccgtgg
     5041 cgaagaacgg ggccagaacc tcaccccggg cttgaacgac gatgaacccc agaatgacgg
     5101 agagaaccgc accggagaga acgatgaacc aggtctcgac aaggaggagc ttaagacaaa
     5161 gagccgccgg gcgccaatcc cgcgcagaag accgatctcg tgatagcgaa ccc
//
LOCUS       DAOSHE010000104         5188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14223_length_5188_cov_3.443600, whole genome shotgun sequence.
ACCESSION   DAOSHE010000104 DAOSHE010000000
VERSION     DAOSHE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14223_length_5188_cov_3.443600"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PLD82_04170"
     CDS             complement(<1..2002)
                     /locus_tag="PLD82_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG64615.1"
                     /translation="MAFSTIKQHDCGYPIFGEAAYCPFCRDRLGEIDVRFQRGETLYS
                     DENRAFVNRAGECTVKSVVLEVRPAGGASSRIQIAGIRIYSDTDDGMCFVQDINQYAE
                     DLSQTVCFDVEKYPCLEFGIDTRDLVVNSGHQVWVNSIELVNSANDVIGVKRVRLETL
                     PVCRASAHLVEYDCNQGKDSIDFDLVLSGIRLSETVSDNHDNAGGLIRSKKIVTTTSG
                     ARLEVTLDRQMAMQMLADNGNQPCIVSIDPEIRLISGTLLDSDPLQIRLRMPPVITIS
                     DWDGNPTPTIEIVPENERIVYVTISNEGRETASIRDLVVDSDRTVEGLECLVSGSSSF
                     DLAPRKSEDDGYKSDRYQIALALRCGSKVPLDQYGFRLCYALRSNNDLEGARRASIPF
                     TVIVKAIASIPTMAIDFGTSNSCVWYEYKAKTLPAPIKKIVDPAAINFFRNLRTLKID
                     KDVMTELDADSHGDGYLRACSIIKTGMLYSMDPSGTTVSTVMDEPDPRSITLFKRDLA
                     SGKTYTISNAAGRQASLTPEQVTTDFLRLLLQSAIKEVGMPERLVVTHPVVFDSHQLK
                     ALKDAVVSVYDGDIRWVDEARAAMFRYALYRKDLLARKIEKGRIRKDQYGETAVVVLF
                     DLGGGTLDVTSARLDMTLSRDGTGGGAEIGKGYFFRGQIQYIG"
     gene            complement(2018..5098)
                     /locus_tag="PLD82_04175"
     CDS             complement(2018..5098)
                     /locus_tag="PLD82_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HOG64616.1"
                     /translation="MAFVNPTIKPAIFIGLGSTGTQVLNYIREFMYEEFGVAGFPVFH
                     FLSISTDGQDETDRQFGIVTQPFERMYEFKIYVPELEGPRAKLDKKNETFFATYSGMH
                     EWFDMDTLKDNRSTVKAGAGGNRQVGRMALWMNWDQFDKHLSQCVDINKDARKDQMRD
                     ILRTYREQKGGNITIDSDLDNAVDVYIVTSLSGGSGSSAFIDVAVAAKRKLSQYFSNL
                     SIYGFFTAMDHTLNDPSHASNCSNTLASLIELDNLHRKGIKIQYPDLPMQTYEDPFSQ
                     VYLMTPSISNEENWSSSVAMHGEKDQDVMRRLMALQVFLSAASGSLIDLGHKINVDIA
                     AMAKGYDLPKAPPDPGFRRFINVMGLYAIWFPKNRILDSASYLLIHDLLRDWLGNEDQ
                     TTIHKADVQKKAADDYTRIESKALGELVADDKRNLDSDLMSRLQDVRVSLMAEDGDAD
                     TLKNKLLTEWGRVEYSNRSLTQEFGSNSHLSEFMNGRAVSMRTSFVTEVKKTVWSKVE
                     EFVKPGEKDLTSRMTLEDTKEWVSSFKTELSQKIEHGYPDTPPRVDIASLDFERVYKV
                     SFWSRITFQKKKAIRKNKEKLYSAFRKKVEDAWRGMKGYHAGRALQQSVKELDESIDK
                     DLTKIKDRLSQLAYDAEKGFQDSCKLEMSENRILIMTMRGGKIKEDAEFIREQVGRIS
                     QELLQKKNADEYLFTRVFLRKMESEKLSVMQQIEQNSMDIKEERSVVLELIRNKLKHI
                     ILREYWKEISGDFSLTKEYLRRHRANILDHYKNSYILAAMSPTWSKLPLKDRPIFAGG
                     GGVHGDDTIKDVLQIVREKDRSAIWEHSFETAEHLDNLIFFARQYPGVAVSDLKLYDE
                     FKPVYESNPSGKVSNHHTSIRPRQFWPTSPWKYDMPALFALIGDAAGLFEQTKDGYAF
                     AYKDGKKEYSILLKNCRFDETTLERAGDMDAWEKSASEAAEVLREKLLAADKEKEFDN
                     RVRSAVIIIKQEQLYEKANQIKSRLIDNGVFDHEQAEEFKNRLYNFVDFMFPKM"
BASE COUNT         1282 a         1361 c         1157 g         1388 t
ORIGIN      
        1 ctccgatgta ctggatctgg ccccggaaaa aatatccctt cccgatctcg gcaccacccc
       61 ccgtgccatc ccgtgaaaga gtcatatcga gccgggccga tgtcacatcc agcgtgccac
      121 cgcccaagtc gaaaagaaca acgactgcag tttccccata ctgatccttg cggatccgac
      181 ccttttctat ttttctggcc aaaaggtctt tccgatagag cgcataccgg aacatggcag
      241 ctcttgcttc atcaacccag cgaatatctc cgtcatagac cgaaaccacc gcatctttga
      301 gcgccttgag ctgatgactg tcaaaaacaa cgggatgtgt cacgaccaga cgctcaggca
      361 tgcccacttc cttgatcgcg gactggagca atagacggag aaaatccgtg gtgacctgtt
      421 cgggagtgag actggcctgg cgtccggcag cattgctgat cgtatatgtc tttccactgg
      481 ccagatcccg cttgaagagc gtgatcgatc gtgggtccgg ttcgtccata acggtagaaa
      541 cagtggtccc cgagggatcc atgctgtaca acatcccggt tttgatgatt gaacaagccc
      601 tgaggtaccc gtcgccatgc gaatccgcgt caagctcagt catgacgtcc ttgtctattt
      661 tgagagtcct gagattccgg aaaaagttga tagcggcagg atcgacgatt tttttgatcg
      721 gtgccgggag tgttttggct ttgtattcgt accagacaca agagttggaa gtgccgaaat
      781 caatggccat tgtcgggatg cttgcaatag ccttgacaat gaccgtaaaa ggaatgctgg
      841 cccgtcgtgc cccttcaaga tcattgtttg acctgagagc atagcacaga cgaaaaccat
      901 attgatcaag aggaaccttc gagccacacc tgagtgcaag tgcgatctgg tatcgatcgc
      961 tcttgtatcc atcatcctca gattttcgag gtgccagatc aaaactggat gatccggaga
     1021 caaggcattc aagcccttca actgtccggt ctgaatcgac aacaagatcc cgaatactgg
     1081 ccgtttccct tccttcgttc gatattgtga catacacaat ccgttcgttc tcgggaacaa
     1141 tctcgatcgt cggtgtcgga ttgccatccc agtccgaaat tgtgatgacg ggaggcatgc
     1201 gaagcctgat ctgaagagga tcggaatcca ggagggtccc gctgatcaac cgaatctcgg
     1261 gatcaataga aacaatacaa ggttgattcc cgttgtcagc aagcatctgc atggccatct
     1321 ggcgatccag tgtgacctca agccgggcac cacttgttgt cgtgacaatt ttttttgacc
     1381 ggatcaaacc acccgcatta tcatgattgt cagaaactgt ttcggacaac cggatcccgg
     1441 acaagaccag atcgaaatca atgctgtctt ttccctggtt gcaatcatac tcgaccaaat
     1501 gcgcactggc tcgacagaca ggcagcgttt ccagccgaac ccttttgaca ccaatcacat
     1561 cattggcact gttgaccagc tcgatagaat tgacccaaac ctgatgaccg ctgttgacaa
     1621 ccaaatcacg tgtatcaatg ccgaattcca gacaagggta tttttctacg tcaaaacaaa
     1681 ccgtttgcga taaatcttca gcgtactgat tgatgtcctg cacaaagcac atcccgtcat
     1741 cagtatcact gtatatcctg atgccggcaa tctgtatacg cgaacttgct cctcctgcag
     1801 gtcttacttc aagaacaaca gacttgacag tacactctcc ggcgcggttg acaaaagccc
     1861 tgttttcatc agaatagagt gtctcacccc gctgaaatcg gacatctatc tctccgagcc
     1921 ggtcccggca aaaagggcaa taggcagcct ctccgaagat gggatagcca cagtcatgct
     1981 gcttgatggt ggaaaacgcc atgtcgtact gccccgctca catttttgga aacatgaaat
     2041 caacgaagtt gtagagtctg tttttgaatt cttcggcctg ttcgtgatca aaaaccccat
     2101 tgtcaatcag gcgcgatttg atctgattgg ccttttcgta cagttgctcc tgcttgataa
     2161 tgatcacggc actgcgcacc cggttgtcga attctttttc tttgtctgct gcaagaagtt
     2221 tctcacgcaa tacttcagct gcttcgctgg cacttttctc ccaagcgtcc atgtcaccgg
     2281 ctcgttcgag agtggtctca tcaaagcgac aattcttcaa caggatagag tattctttct
     2341 tgccgtcctt gtaggcaaaa gcatagccgt cctttgtctg ctcgaacaac ccggcggcat
     2401 cgccaatcag ggcaaaaaga gccggcatgt cgtatttcca tggagatgtg ggccaaaatt
     2461 ggcggggtcg aatacttgtg tgatgatttg acactttgcc ggacggattg ctttcataga
     2521 ccggcttgaa ctcgtcatac aacttgagat cgctgacagc aacacccggg tactgtctcg
     2581 caaagaaaat cagattgtca agatgttctg ctgtttcgaa agaatgctcc cagatagcac
     2641 tgcgatcttt ctctcgtaca atctgaagca catcctttat cgtgtcatca ccgtggactc
     2701 caccaccacc tgcaaaaatc ggtctgtctt tgagaggcag cttgctccac gtcggactca
     2761 tcgccgccaa aatgtatgaa tttttgtaat gatcaagaat attggctcga tgacgacgca
     2821 ggtattcctt tgtcaggctg aaatcccctg aaatttcttt ccagtattca cgcaggataa
     2881 tgtgcttgag tttatttcgg atcaactcga gcacgacact tctttcttcc ttgatatcca
     2941 tggagttttg ctcgatctgc tgcatgacag aaagcttttc actttccatt ttacgcaaga
     3001 aaacccgtgt gaacagatat tcatcagcat ttttcttttg cagcaattcc tggctgatcc
     3061 tgcccacttg ttcacgaata aattccgcgt cctctttgat cttgcctcca cgcatggtca
     3121 tgatcaaaat ccggttttca ctcatctcaa gtttgcatga atcctgaaaa cctttttccg
     3181 catcatacgc caattgtgaa agacgatcct tgatcttggt cagatcctta tcaatacttt
     3241 catcgagttc cttgactgac tgttggagcg cccggccggc gtgatagccc ttcatgccgc
     3301 gccatgcgtc ttcaaccttt ttcctgaatg cagaatacag cttctctttg tttttcctga
     3361 tcgccttttt tttctggaat gtaatcctgg accaaaaact gaccttgtac acccgctcga
     3421 agtcaagaga cgcaatgtca acacggggag gagtatccgg atatccatgc tctatttttt
     3481 gggagagctc ggttttgaat gaactgaccc actcttttgt atcttcaagc gtcatgcggc
     3541 tggtcagatc cttttcgcca ggctttacaa actcttcgac cttgctccaa accgttttct
     3601 tgacttcagt cacaaaactt gtgcgcatgc tcacggcacg accattcata aactctgaca
     3661 ggtgactatt gctgccgaat tcctgtgtca gggaacgatt gctgtattca acccggcccc
     3721 actccgtcaa gagcttgttt tttagcgtgt ctgcatcgcc atcctcagcc atgagactca
     3781 cccgaacatc ctgcaatcgt gacatgagat cactgtcaag attgcgtttg tcatctgcca
     3841 caagctcgcc aagcgccttt gactcgatcc gggtataatc gtctgcagct tttttttgaa
     3901 catcggcctt gtggatggtt gtttgatctt cattgcccaa ccaatcacgg aggagatcat
     3961 gaatcagcag gtaggatgca ctgtccaaaa tcctgttttt gggaaaccag attgcataga
     4021 gacccatcac attgatgaac cgtcggaagc ccggatccgg tggcgccttg gggagatcat
     4081 aacccttggc catagcggca atatcaacat taatcttgtg tcccagatcg attaatgagc
     4141 cagaggcagc agaaagaaac acctggagtg ccatcagtcg ccgcatcaca tcctgatcct
     4201 tttctccatg catggccacc gaactggacc agttttcctc atttgaaata gaaggcgtca
     4261 tcaaatatac ttggctaaaa ggatcctcgt aggtctgcat gggaagatcc gggtactgga
     4321 tcttgatgcc tttgcgatgg agattgtcga gctcaatcaa tgacgccaga gtattgctgc
     4381 agttggatgc atggctggga tcgttgagag tgtgatccat ggccgtaaaa aagccataaa
     4441 tcgacagatt gctgaaatac tgtgaaagct tgcgcttggc ggctaccgca acatcgataa
     4501 aagccgaaga cccggatcca ccagaaagtg atgtgacaat atagacgtca acggcgttat
     4561 caagatcact gtcaatcgtg atattcccgc ccttttgctc acgataggta cgcagtatat
     4621 cccgcatctg atcctttcgt gcgtccttgt ttatatccac gcactgtgac aaatgcttgt
     4681 cgaattgatc ccaattcatc cagagcgcca tccgcccaac ctgacggttt ccaccagccc
     4741 ctgccttgac tgtggaacga ttgtctttga gcgtatccat gtcaaaccac tcatgcatcc
     4801 cgctatacgt ggcaaaaaat gtctcgttct ttttgtcgag cttggctcgc ggaccttcca
     4861 attcaggcac atagattttg aactcgtaca tgcgctcaaa aggttgtgtc acaattccaa
     4921 attgacgatc tgtttcatcc tgcccatccg tactgatgct gagaaaatga aagaccggaa
     4981 atccggccac cccgaattct tcatacatga attcacgaat gtaattcaaa acctgggttc
     5041 cggttgatcc aagcccgata aaaattgccg gtttgattgt aggatttaca aaggccatgg
     5101 gatacgttct ccttagatga gattagtctt gtttgagaat ttctatacga acagggactt
     5161 ccttgccaaa cttgtttttg agcacgac
//
LOCUS       DAOSHE010000105         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14230_length_5186_cov_4.546872, whole genome shotgun sequence.
ACCESSION   DAOSHE010000105 DAOSHE010000000
VERSION     DAOSHE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_5186_cov_4.546872"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..573
                     /gene="tpx"
                     /locus_tag="PLD82_04180"
     CDS             70..573
                     /gene="tpx"
                     /locus_tag="PLD82_04180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOG64617.1"
                     /translation="MAEITLHGNTIHTCGALPAVGTRAPEWVLTDSELADRSSTDFSG
                     KTLVLNIFPSLDTPVCAASVRRFNAEAAARKNVAVLCISLDLPFAHKRFCATEGLDAV
                     TGLSELRQRAFGEAYGVRIIDGPLAGVLARAVVVVDLQGIVRYTELVPEIAQEPDYAA
                     ALAAIGV"
     gene            763..1182
                     /locus_tag="PLD82_04185"
     CDS             763..1182
                     /locus_tag="PLD82_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG64618.1"
                     /translation="MAEDFVVWEDRFNVGIVMIDEQHRKLVDFTNALFDGCRVGGDGA
                     NEAFRKVLRDVVAYVKVHFSSEEQLMQEMGWDGYAEHKKKHEEFVRRVLEDVANFEGG
                     QKFVPNQFVRFLRDWLLEHIAVADHEFSGWALPRGAK"
     gene            complement(1248..1625)
                     /locus_tag="PLD82_04190"
     CDS             complement(1248..1625)
                     /locus_tag="PLD82_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG64619.1"
                     /translation="MPKKQSTSFSAPCQECGGRCCRYIALGIDPPKNRTARENIRWYL
                     LHENVSVFVDHEKGWYVEFTTPCTAQAPDLRCTVYQNRPKICQDYGNAHGDCEYYDNP
                     YALRFEHLEEFDAWIANKQRRRS"
     gene            complement(1907..2203)
                     /locus_tag="PLD82_04195"
     CDS             complement(1907..2203)
                     /locus_tag="PLD82_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64620.1"
                     /translation="MPSPDPIDCREAFDRQLARFKPWLRYCLVWTMVAGLTPLVLLML
                     GRLDTYPVLAPWIYGAILPALWAIWQAGRCPRCRRSLPNSRDPESCPHCGCRLR"
     gene            2501..2926
                     /locus_tag="PLD82_04200"
     CDS             2501..2926
                     /locus_tag="PLD82_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG64621.1"
                     /translation="MIKLSTRSRYGVRFLVDLARHSGTASLREVAEREGISEKYLWSM
                     VPPLKNAGLISTARGAGGGCSLAKPAGRITLREVIEILDGGIRLADCTMGQGGCDRVG
                     RCGMATVWMEIEKQLEAVLEGHSIESIAHGTADVGGYCI"
     gene            2960..4255
                     /locus_tag="PLD82_04205"
     CDS             2960..4255
                     /locus_tag="PLD82_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOG64622.1"
                     /translation="MENKISLDTILVHAGQTPDPVTKSRAVPIYQTSSYVFEDSQDAA
                     DLFALKKFGNIYTRLMNPTTDVFEKRVAALDGGVAALAVASGQSAISLALLNIAQAGD
                     EIAAAENLYGGTWSLFKHTFKRFGIGVRFFDPDDPASLANALTQKTKAVYAETIGNPR
                     LNVSDLSRLAETAHAHGVPLVVDNTVAPYLCRPIQYGADIVVYSATKFLGGHGTSLGG
                     VIVDSGKFDWTNGRFPLIAGPDASYHDLDFVEALAPMGNIAFIIKARVSLLRDLGPAL
                     SPFNAFLLLQGLETLHLRMPRHSANAFLVAKFLQRHPGVAWVNYPGLPESPESARAEK
                     YLSNGAGGIIGFGIKGGLEAGRRFIDALRLFSHLANIGDAKSLAIHPASTTHQQLSEE
                     EQSSVGVTPDFVRLSIGIEGVDDLVLDLDQALQQATSPK"
     gene            4340..>5186
                     /gene="cysK"
                     /locus_tag="PLD82_04210"
     CDS             4340..>5186
                     /gene="cysK"
                     /locus_tag="PLD82_04210"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG64623.1"
                     /translation="MSRIHESVVDTIGHTPLVRINRLGAGLPGSVLVKLESFNPLSSV
                     KDRIGAAMVRAAEESGRLAPGGTIIEPTSGNTGIALAFVAAARGYRLVLTMPETMSVE
                     RRQLLRILGAELVLTEAARGMQGAVDKARELTESTPGAVMLQQFENPANPAAHVSSTA
                     LEIWNDTDGAVDVFVAGVGTGGTITGTARVLKEKKPGVRIVAVEPAESPMLSEGRPAP
                     HKIQGIGANFVPAVLERGLIDEILTVTAEEAGEMTRRLAREEGILAGISSGAALTAAL
                     RVAARP"
BASE COUNT         1044 a         1290 c         1707 g         1145 t
ORIGIN      
        1 gtccgacctt gttgcttgca aaaacacggg tggtcgggag ataatcaggg gtaacagagg
       61 aggacgagaa tggccgagat aaccttgcat ggaaacacga tccacacctg tggggccttg
      121 cccgctgtag gcaccagggc gccggagtgg gttttgacgg acagtgagct ggcggatcgc
      181 agttcgaccg atttttcggg gaagacactg gtgctgaata tctttcccag tctggacacg
      241 ccggtctgtg cggcatcggt gcgccggttc aatgccgaag cggccgcgag aaaaaatgtt
      301 gccgtcctct gcatctccct ggatctgccc tttgcgcata aacggttttg tgctaccgag
      361 ggtctggatg ccgtgacggg attgagcgaa ttgcggcagc gcgcttttgg cgaagcctat
      421 ggtgtccgga tcattgatgg accgttggcc ggagttttgg cgcgggctgt ggtggttgtc
      481 gatctccagg ggatagttcg gtataccgag ctcgtccccg aaattgcgca ggagcccgac
      541 tacgcggccg cgttggcggc catcggggtg tgatggcgtc gtgtttccat gtgcggcgcc
      601 ctgtctgcag tccgggaaca gcagatgtga tccgggccgg tgacagagtc tccgcgcggg
      661 tcagcgggct gatcatagag aaagagtcat atacttcttg catcttctgt cggggcatgc
      721 ctatccttct ggcagggatg gagaaacccg caggagcgtc ctatggctga ggattttgtt
      781 gtctgggaag accggttcaa tgtagggatt gtgatgattg acgagcagca tcgcaagctg
      841 gtcgatttta ccaatgccct ctttgatggc tgtcgagttg gtggcgacgg tgccaatgag
      901 gcttttcgga aggttctgcg ggatgtcgtg gcctatgtca aagtccattt ttcgagtgaa
      961 gaacaattga tgcaggagat gggctgggac gggtacgcgg aacacaaaaa gaaacatgaa
     1021 gagtttgtgc gacgggtgtt ggaagatgtc gcgaatttcg agggcggtca aaaattcgtt
     1081 ccaaaccagt ttgtgcggtt cttgcgggac tggctgcttg agcatatcgc ggtggcggat
     1141 cacgagtttt caggatgggc gcttcccaga ggggcgaagt aaggcttggg gaacattttc
     1201 cggacacaaa atggcgtttc ttgcccgagg ttgttcacca aaaagcatca tgagcgtcga
     1261 cgctgtttgt tggcgatcca ggcgtcgaat tcctcgaggt gctcgaagcg gagtgcgtag
     1321 ggattatcgt agtactcgca atcaccatgg gcattgccgt agtcctggca gatcttgggg
     1381 cggttttggt agacggtgca gcgcaggtcg ggtgcctgag ccgtgcaggg cgtcgtgaat
     1441 tcgacgtacc agcccttttc gtggtcgaca aaaacggaga cgttttcgtg caggagatac
     1501 cagcggatat tttcgcgggc cgtccggttt ttgggcgggt cgatcccgag agcgatgtag
     1561 cggcagcagc ggccaccgca ttcctggcaa ggtgctgaaa aacttgtgga ctgttttttg
     1621 ggcatatctc ccccggatgc atgacggatc aggatgtatg acgtgaagta taccgtttgc
     1681 gcccgagcgt ggtgctgctt aaattttaag caattgtgca atccgggatt gcatcgttgt
     1741 ctgtaaaaaa aagcagaatc atgaaaaacc cctgtaaatt cgcgaatctt tcagggtttg
     1801 gtcaaaaaat gcaggtgagc cactgcttaa attttaagca attgtgcaat ccgggattac
     1861 tttgcgtcgt ggcgtcgtgg cgtcgtggcg tcgtggcagc gaaggatcag cgcaggcggc
     1921 agccgcagtg gggacaggac tccggatcgc gggagttggg aagacttcga cgacagcgtg
     1981 ggcagcgtcc ggcttgccag atggcccaga gagccggcag gatggcacca tagatccagg
     2041 gcgccagaac cggataggtg tcaaggcggc cgagcatgag cagaacaagg ggtgtcaggc
     2101 cggcaaccat ggtccataca aggcagtagc gcagccaggg cttgaagcgg gccagctggc
     2161 ggtcaaaggc ttccctgcaa tcaatggggt ccggcgaagg catctcttct ccttagggtc
     2221 ggcgatttct gcaagagctg ggtaagacac catggtgcat gcatcagtat agcacaggat
     2281 tggccatgtg tacgctgttt tcccgaatat gggcgcatga agtcgtgtgt gaaaggtgct
     2341 tttctgcacc atggtgtacg gggaaggcgc ataccgagag tttttgccgg tgatgtacac
     2401 aggattgaga caaaacacca tggtgcacga attttctcct tgcagttcgt tctaaaatct
     2461 agtaatacta tcgatatgct agtgtataaa ggaggtgcgg gtgatcaaac tttcaacacg
     2521 ttcgcgctat ggtgtccggt ttctggtcga tcttgcccgg cattccggga cagccagtct
     2581 gcgtgaggtt gcggagcgcg aggggatctc ggagaaatat ctctggagca tggttcctcc
     2641 gctcaagaat gccgggttga tcagcacggc acggggggcc ggcggcgggt gttctctggc
     2701 caaaccggca gggcgaatca cccttcggga agtaattgag attctggatg gcggtatccg
     2761 gctggcggat tgcaccatgg gtcagggcgg gtgtgaccgt gtcgggcgtt gcggcatggc
     2821 tacggtctgg atggaaatcg agaagcagtt ggaggccgtg ctcgaggggc atagtatcga
     2881 gtcgatagcc catggtacgg ccgatgttgg tggatattgt atctgatttc gggatcaaga
     2941 aaaaaaggag gaagtgcgaa tggaaaacaa aatctcgttg gacacaattc tggtgcatgc
     3001 cggtcagact cctgatccgg tgacaaagtc gagggctgtg cccatctatc agacctcatc
     3061 gtatgtcttt gaggattcgc aggatgcagc ggatctcttt gcgttgaaga agttcggcaa
     3121 tatctatacc cggttgatga atcctacgac cgatgtattt gaaaaacggg tggcggccct
     3181 tgacggtggt gtggcagcac tggccgttgc cagcgggcag tcggcgatca gtctggccct
     3241 cttgaacatc gcccaggccg gcgatgagat cgcggcggca gagaatctct acggggggac
     3301 ctggtccctc ttcaagcata cattcaagcg tttcgggatc ggggtgcggt tttttgatcc
     3361 ggatgatccc gcctctctgg caaacgcctt gacgcaaaag accaaggcag tctatgcgga
     3421 gacgatcggg aatccgcgcc tgaacgtgag cgatctctcg cggttggccg agacggccca
     3481 tgcacacggg gttcctctgg tcgtcgacaa tacggttgct ccctacctct gccggcccat
     3541 ccagtacggc gccgatatcg tggtctattc cgcgacaaag ttcctcggcg ggcatggcac
     3601 ctctttaggt ggcgtgattg tcgattccgg taaattcgac tggacaaacg gccggtttcc
     3661 cttgatcgcc ggtccggacg cgtcctatca tgatctggat tttgtcgagg cactcgcccc
     3721 gatggggaat atcgccttca tcatcaaggc tcgtgtcagc ctcttgcgtg acctcgggcc
     3781 ggccctctcg cccttcaatg ctttcttgct tctgcagggg ttggagacac tccatctcag
     3841 gatgccgcgc cattccgcca atgcctttct cgtggccaaa tttttgcagc gccatccggg
     3901 cgtggcctgg gtcaattatc ccggcttgcc cgagagtccc gagagtgcgc gggcggaaaa
     3961 atatctctca aacggggccg gcgggattat cggattcggg atcaagggtg gtctcgaggc
     4021 ggggcggcgt tttatcgatg cgctccggct cttctcgcat ttggcgaata tcggagacgc
     4081 caagtcactg gcgatccatc cggcgagtac gacacatcaa cagctttctg aggaggaaca
     4141 gtcttccgtc ggagtaacgc ccgattttgt ccggctttcg atcggtatcg agggggtgga
     4201 tgatctcgtg ctggatctcg accaggcctt gcaacaggca accagtccca agtgatgaag
     4261 acgccgcaag acgcgattgt cgtggtggaa aaaatccacc ggaatggcct gaggctgttc
     4321 acaaaaatgg aggtttgcaa tgtcacgaat acatgaatcc gtagtagata cgatcgggca
     4381 caccccgctg gtgcggatca acaggctggg cgcaggcttg ccgggatcgg tgcttgtcaa
     4441 actggaatcc ttcaatccgc tctccagcgt caaggaccgg atcggggccg ccatggtgcg
     4501 cgctgccgag gagagcgggc ggctggctcc gggtggcacc atcatcgagc cgaccagcgg
     4561 aaacaccgga atcgctctgg cctttgtggc cgcggcccgg gggtatcgcc tggtgttgac
     4621 gatgcccgag acgatgagtg ttgagcgccg gcagttgttg cggattttgg gtgccgagct
     4681 tgtcctgacg gaggcggccc gcgggatgca gggtgcggtg gacaaggccc gtgagctgac
     4741 cgagagcacg cccggggccg tcatgctcca gcagtttgaa aacccggcca atccggccgc
     4801 ccatgtttcc tcgacagcgc ttgagatctg gaacgatacg gatggggctg tggacgtgtt
     4861 tgtggccggc gtggggaccg gagggacgat taccgggaca gcgcgtgtcc tcaaggaaaa
     4921 aaagcccggc gtgcggatcg tggcggtcga gccggccgag tcgcccatgc tgtccgaagg
     4981 caggcccgcg ccgcacaaga tccaggggat tggtgccaat tttgttccgg ccgtactcga
     5041 gcgcgggctg attgacgaga tcctgacggt gacggccgag gaggccgggg agatgacgcg
     5101 gcgcctggcc cgcgaagaag gcatcctggc gggaatttca tcaggggctg cattgactgc
     5161 tgcgctgagg gtcgcagcca ggccgg
//
LOCUS       DAOSHE010000106         5182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14245_length_5182_cov_3.278916, whole genome shotgun sequence.
ACCESSION   DAOSHE010000106 DAOSHE010000000
VERSION     DAOSHE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14245_length_5182_cov_3.278916"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1827
                     /locus_tag="PLD82_04215"
     CDS             553..1827
                     /locus_tag="PLD82_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64624.1"
                     /translation="MKTCRILALAVLLMLLALIPVTAATYTRASQAGSPGGPHDFTGI
                     IYYGHSAESISFRDAANGSIIVEIPANIMPNVRSAVLAYRHRPFNIRIQITSPYNQRT
                     QGCGGSYIGHSLTPSQPGSGTVNLARSVNRTGGSTIRNVTYQSHTATSISFRDTEGNT
                     AVAVILTHQLNDLLNQVLANRNTRYDLTLEVTHPYSAASRECRGTYRNHLVSQGSDGQ
                     QTSSAATRSGTITVNTASAITDTGNWAIRNLTYLRHTDVYFYFRDSANYAVDIAVPQN
                     QRQTLISQIAGNPSRLYTVTMQSRGAYNQNASRIIGTYVSHTAQAADSGNAGQQGNRN
                     IRSLSEATITGEYTIASITYVSVARGGLVYTFRDFDNNTLTLRMADVAQSDRLWDYLN
                     SRNNPRASIRMQVTHPYNAQNRQCQGTYIAHY"
     gene            complement(1935..2420)
                     /locus_tag="PLD82_04220"
     CDS             complement(1935..2420)
                     /locus_tag="PLD82_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64625.1"
                     /translation="MIIGPDTFTNSVTWSGPATARDRIALTAKRIIGWGRGGYVYFGS
                     SNGLGLPIGYGNGVWGYVLRLQVLEPTSGCWQTPYQVTMTNNVTTLSTQSVQSVMIPV
                     LTQGVKALVSYTNTVVRSSIVTLETNGHSSVLWGCTENPARMTENGKRLLFNLVEASS
                     L"
     gene            complement(2417..2641)
                     /locus_tag="PLD82_04225"
     CDS             complement(2417..2641)
                     /locus_tag="PLD82_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64626.1"
                     /translation="MKQGLVVLGVVLLLVVACGDDEDGASAKGTIAYVYNTNISIWPP
                     LEPSFRSTATDTGLYRPPRPLKATFQIVRP"
     gene            2890..3153
                     /locus_tag="PLD82_04230"
     CDS             2890..3153
                     /locus_tag="PLD82_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HOG64627.1"
                     /translation="MGFIFRKSVNLGPIRLNFGKNGIGVSTGIPGLRVGRNSKGKTYG
                     SAGIPGTGMSWRTSQKGAKTGGSGCLVMLVAGLVAASILTNLL"
     gene            complement(3286..4122)
                     /locus_tag="PLD82_04235"
     CDS             complement(3286..4122)
                     /locus_tag="PLD82_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG64628.1"
                     /translation="MGHLLILVCTMLVLLPIPGLGSRELPGVLDDFPATIGEAHSGNN
                     LFWHGAAVGSTVFFVASGADRDWQGWLQENGILGRGFENPVLFAGNFWTIGIALWLYL
                     DGRAGDDPVTAGAGVTAFQAVVGTFLVVTTEKFLTGRRGPAGPNDTPDMPFVKTDDPA
                     DFAFDFWNSSFSDGRFFWPSGHTASAFSLVSALVSYYPDEDWLAWLGYPLAALMGYVM
                     MEGDYHWLSDVVAGACIGHVIGWTAGRRARGRISGVTDTTLEIVPVIGRDSAGLALRI
                     GM"
     gene            4336..4512
                     /locus_tag="PLD82_04240"
     CDS             4336..4512
                     /locus_tag="PLD82_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64629.1"
                     /translation="MMNEDELRSYNKAQIDEILRYKWIESEKQGQDIGEMRAATEWIS
                     KYSADFRKQYCGEG"
     gene            4755..>5182
                     /locus_tag="PLD82_04245"
     CDS             4755..>5182
                     /locus_tag="PLD82_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG64630.1"
                     /translation="MSPFSLRIADRVASVQPSATLTISTKAKELAASGKNVIDMSAGE
                     PDFNTPEFIIETAYSAMKKGKTKYTAVPGTIDLRKAISEKFKKENGLDHPPELISVAN
                     GAKHTLFNIIQAILNPGDEILIPTPDWVSYKEMAHLASAIP"
BASE COUNT         1270 a         1589 c         1303 g         1020 t
ORIGIN      
        1 cagccatgca tagcgccaag acaatcatca cgattgtacg cattttcacc ctccgcacca
       61 tccgtgtatc acatccatag ttttcccgaa ttctgttttc gtcaacaaag aagtgatttc
      121 cagatcatcg cagagacacg atgaggctag aaccatctgg tgtgggcagt atggtgaaca
      181 tgcacaccgt caagaattcg gtcatcccga aacgcgatgg atcccgtctc tgcggttgac
      241 tgaaaacaca agatccccgc cggacccgag cgtcaatctt ggccaataga aaagagcctt
      301 ctgcggatcg tctatccgat actcgatttc aaaatcaaat accatgacga gagtcacatg
      361 acacttttat caaatcggca tcacaaagtc gcgataaatc ccatctgcat gaagacaggc
      421 cagcatgatg aggatgatcg gcctgccatg ccatcattgt cttttgcgcg tgacaaatcg
      481 gggctgaatg tgtttatgat ggaacacact gcatccttgc tgaacccgag attcccattc
      541 aaaggagctt gtatgaaaac atgtcgtatc ctcgcgttgg ctgtcctcct catgctgctc
      601 gcccttatcc cggtgacggc cgcgacctac acgagagcca gtcaggccgg ttcccccggt
      661 ggcccccacg actttactgg tatcatctat tacggtcact ccgccgagag catctcgttc
      721 cgtgatgccg ccaacggctc gatcatcgtc gagattcccg ccaacatcat gcccaatgtc
      781 cgcagtgcgg ttctcgccta tcgccaccgg ccattcaata tccgcatcca gatcacctcg
      841 ccctacaatc aacgaaccca ggggtgcggc ggttcctata ttggtcacag cctcacgcca
      901 agccaaccgg gcagcggcac agtcaatctg gcccgctcgg tgaaccgtac cggaggcagc
      961 accatccgca atgtgaccta ccagagccat acggcaacct cgatttcatt ccgcgatacc
     1021 gaaggcaaca cggctgtggc ggtcatcctc acccaccagc tcaacgacct cctcaaccag
     1081 gtcctggcaa accgcaacac gagatacgac ctgacccttg aagttaccca tccctacagc
     1141 gccgcgtccc gcgaatgccg tggtacctat cgcaaccacc ttgtatcaca gggttctgac
     1201 ggccagcaga caagcagtgc cgcgacccgc agcggaacca tcacggtcaa taccgccagc
     1261 gcaataacgg acacagggaa ctgggcgatc cgcaatctga cctatctccg gcacacggat
     1321 gtgtactttt acttcaggga cagtgcaaat tatgcggtcg atatcgccgt accccaaaac
     1381 caacggcaaa cccttatttc acagattgcg ggcaatcctt cccgactgta taccgtgacc
     1441 atgcaatcgc ggggcgcgta caaccagaat gccagcagga tcattggcac ctatgtgtcg
     1501 catacggcac aggcggccga ttcgggcaat gccggacagc agggaaaccg caatattcga
     1561 tcgctcagtg aagccacgat aacgggtgaa tacacgatcg catccataac ctatgtcagt
     1621 gttgccaggg gcggcctggt ctataccttc cgcgattttg acaacaatac cctgaccctc
     1681 aggatggccg atgtggccca gagcgaccgc ctttgggatt acctcaattc gaggaacaac
     1741 ccgcgcgcct cgatcaggat gcaggtcacg catccgtaca acgcccaaaa caggcagtgc
     1801 caaggcacgt acatcgcaca ttattgattc tcaaagattg ttttgaggaa cagagtccac
     1861 ccccgggatt accgttgttt ccccgggggt ggattgcata cgtgttggat gcggcaggcc
     1921 tggcacgccc gggtctacag tgaggatgct tcgaccagat tgaagaggag ccgcttgccg
     1981 ttttcggtca tccgggccgg gttttcggta catccccaga gcacagagga atgcccgttg
     2041 gtctcgagcg tcacgatcga ggaccggacg acggtattgg tgtacgaaac cagggctttg
     2101 acaccctgcg tcaggaccgg gatcatgacg gactggaccg actgggtaga gagcgtcgtc
     2161 acattgtttg tcatcgtgac ctgatacggt gtctgccaac agcctgacgt cggttcgaga
     2221 acctggagac gcaggacgta cccccagaca ccattcccgt atccgatcgg caggccgagt
     2281 ccgttggaac tcccgaaata aacatagccg ccacgccccc atccgatgat ccgtttggct
     2341 gtcaaggcaa tcctgtcacg agcagtcgcc gggccactcc aggtgaccga attcgtgaag
     2401 gtatccggcc cgatgatcac ggcctgacaa tctgaaaagt cgctttcagc ggcctgggcg
     2461 gccgatacaa accggtgtcg gtagccgtac tcctgaaaga gggttccaaa ggcggccaga
     2521 tcgaaatatt ggtattatac acataggcaa tggttccctt tgccgaagcc ccgtcctcat
     2581 catcaccgca ggccactacc aacagcaaca ccacacccaa cacgaccaga ccctgtttca
     2641 tgccgcacct tcgcatgaaa caagcgtaca accttgcaca gagatttcac gcctgttcaa
     2701 ggacactaca tcaggctaaa ccccgtcgac ccgggcatgg acggctcttc ctgggtaggc
     2761 ccgcacaatt gattcgaacc ggtgaaaccg ggatccaggc agaaactcca ggtttggcgt
     2821 gtctgcaatc aaatcttttc tccaatcgta tcatgtgcta tactggtcaa aatctggagg
     2881 gatttttgta tgggattcat cttccgcaaa tcggtcaatc tcgggccgat cagactcaat
     2941 ttcggaaaaa acgggatcgg agtatccacg ggcattcccg gcctgagagt cggccgcaac
     3001 tccaagggca agacctacgg ctcggccgga atcccgggca cgggcatgtc ctggcgtaca
     3061 tcacaaaagg gagccaaaac cggaggatca ggctgtcttg tgatgcttgt tgccgggctt
     3121 gttgccgcaa gtatcctgac aaacctgctt tgattcgccc ggagttcaaa tcggatacac
     3181 ttccaacgac cgtgcaaacc tctggctcct tctccgctga acaggaaggt ttgccacaca
     3241 cacgcgttcc aacagagtcg cccctgatcg gccctcgccg gaagttcaca tcccgatccg
     3301 gagcgccaga ccggcggaat ccctcccgat caccgggaca atttcaaggg tggtatctgt
     3361 cacgcccgag atccgtcccc gggcacggcg gccggcggtc cagccgatca catgcccgat
     3421 gcaggctccg gctaccacat ccgacagcca gtgataatcc ccctccatca tgacgtaccc
     3481 catcaaggcc gccaggggat accccagcca ggccagccag tcttcatccg gataatagga
     3541 gacaagggcc gagacgaggg aaaaggccga tgccgtgtgt ccggagggcc aaaaaaagcg
     3601 tccgtcgctg aacgatgaat tccaaaaatc gaaagcaaaa tccgcgggat catccgtctt
     3661 gacaaacggc atatcgggcg tatcgtttgg tccggccggg ccccggcgac cagtcaggaa
     3721 cttttcggtc gtcaccacca gaaaggtgcc gacaacagcc tgaaacgctg ttaccccggc
     3781 cccggccgtg acaggatcat ccccggcccg gccgtcgagg tagagccaga gtgctattcc
     3841 aatcgtccag aaattcccgg caaacagaac gggattctcg aatccgcgtc ccaatatgcc
     3901 gttctcctgc aaccagcctt gccaatcacg atccgcgccg gatgcgacaa aaaaaacagt
     3961 cgatccgaca gcggccccat gccaaaacag attgttcccg ctgtgcgctt cgccaatggt
     4021 cgcgggaaaa tcgtccagca ctcccggcaa ctcccgggat ccgagtccag ggatcgggag
     4081 caagaccagc atggtacaca cgagaatcaa caaatgtccc atcatagcct ccccgtttgt
     4141 gcatcttcgt cggtgtcccg tgaaatgtca agcctgtggc tttcaccctg attttggtgt
     4201 atttttatgg aagaaatgca tacccaaaga ggtgattgaa cttatatttt cgttgacttt
     4261 taccgaatgg ataggcagac tactacaacc ggagaaatca cagcccaaaa ccgtggagat
     4321 tgtggagctg tttgtatgat gaacgaagac gaactgcgat cctataacaa ggcccaaatt
     4381 gacgagatcc tgcgctacaa gtggatcgag agtgaaaagc agggtcagga catcggtgag
     4441 atgcgtgccg ccaccgagtg gatctccaag tacagtgccg atttccggaa acagtactgt
     4501 ggcgagggtt gagtcatccc tcctcgcggg atcttccgtc cggtccgtgt cccgtatgca
     4561 cccggcctga gggtgttcac caaaaaaaaa ttgactgatt gccggaattt cggtagatat
     4621 tgcaccgaag cccgagttct ccgcagaccg gcggagactc aacccggata caaggcaggg
     4681 cgacacgacc ctcgtctgcc gctccgggct tcccgaaaca gccgtacaaa ccgaaccttc
     4741 ctggagcccg tacaatgagc ccgttttccc tccgcattgc cgatcgcgtc gcatccgtcc
     4801 agccctcagc aacgctgacc atctcgacca aggccaaaga actggccgcg agtggcaaaa
     4861 atgtcatcga catgtctgcc ggtgaacccg acttcaacac acccgagttc atcatcgaaa
     4921 cagcctattc cgccatgaaa aagggaaaga ccaagtacac tgcggttccc ggtaccatcg
     4981 acctgcgcaa agccatcagc gaaaaattca aaaaagaaaa cggtttggac cacccgcccg
     5041 agctgatctc ggtcgccaat ggtgccaagc ataccctctt caacatcatc caggcgatcc
     5101 tcaaccccgg tgacgaaatt ctgatcccga caccggactg ggtttcgtac aaggagatgg
     5161 cccacctggc cagtgccatc cc
//
LOCUS       DAOSHE010000107         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14266_length_5176_cov_3.843390, whole genome shotgun sequence.
ACCESSION   DAOSHE010000107 DAOSHE010000000
VERSION     DAOSHE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14266_length_5176_cov_3.843390"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="PLD82_04250"
     CDS             <1..2096
                     /locus_tag="PLD82_04250"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOG64631.1"
                     /translation="ERLYTLLTNPAILAGSGLARNAIETWFQALAELGSFRGETGLEG
                     KPDSFTFAKGLTRLRLVRVMGDQPWLDLVPSGACDPELAGRVMVLVEQLESTIRQLEG
                     QRQTGSRWRKTLLALAETFLGLPGQTIMAEAGARKQALQLLRELDWLPECGFDTIAAW
                     AAGRLGSITLPPARGGGVIVAGMQDVRPVPFRLVYLLGMGEGLFPTPWKPASPLDFLA
                     KDNDRFPGDIEDAERDRMLFVETLLAVRQKLVISYIDRDILKDSTRYPSSLISELRQL
                     ADIPDEAFLSIPVTGWAVTPEATALDARAPDIMSDYIRLNAGMARLRVHPEEAEAARI
                     AAVTSEREEQKSIDLSSIKRVIENPSAGILRYAYRVEKTAGEALSLEADEPFIETDAG
                     NESNLWTLASDLTIGWLLAEGEEPFGDFLKACGRERFISGYQRCDRVSAGFFGELIWQ
                     KKEKDLLGQAEGLGQIRTRLRAALRATNPLWSPFVFGPAPTAAREIVRGDLLPGVRLR
                     ENLVLGGILPLVWLEDGGKRICLFVDRKFGAQKVANTSVLNLIPQLVVALPSVRQALG
                     LDGQAEVLLVSYEAPGGIPLFPGTEQEACLQRVLDLLEPGTAEQAPAMLLPAKKILAD
                     DKRIPDAEGLLVALQNAEENSHDRFVLNPAQVLVQPVVPDTDGIKTWCTSMLAPWIRM
                     LKPDWVIPTSEDDDE"
     gene            2116..>4189
                     /locus_tag="PLD82_04255"
     CDS             2116..>4189
                     /locus_tag="PLD82_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG64632.1"
                     /translation="MQCPEDVDLTRHAVIEASAGTGKTWTIEQLVARMVSGDGDSAKG
                     LDLRQILVVTFTERAAGEMRDRIRATLVDLAKDKPELRAWLESCLSAFDEAMITTIHG
                     FCRRVLSEYALECGLPGSPELVDDTAVHSAMLRRIYRDWLADPGQEVLARLAGFSDDP
                     ERYQKNLLRLAGLEDVVPPGEGPVTSYDELPGLLELINKEFVYAMQLLKPFEDEIDRL
                     CQVKTLAGHHGTYYTRIRTSWLVIRECLAVDLERLSRQDLAVCMLQIHSAVVEKGSDF
                     KGYQKQYHDLTGACVLKVKLTIADCAPDACWPGIIPCLDEVIDRLETVRKGLAEIRRS
                     HLYAVAERVRRMARAEKAARDQYSYNDMIRLVRHAVERPGRGLTARLRERYRVAMIDE
                     FQDTDLAQWEIFRQVFAESDEGHTLCLIGDPKQAIYGFRGGDVATYFSARSYLVERKG
                     ARVYTLGTNWRSNTAMVQACNALFASTDDGPVWFLPHPGSGEAPDFLPSDVPKQPRND
                     WAGPASPDPIVVQEIGRGNAAANRFAAAGWIADRIGAILRGALPEVRLAGTKEARPLG
                     AGDIAILVRAKGEAEPVLRVLRARRIPCTFYKAGGLWRSPEALAVVTLFDALALGTPQ
                     AIRLALLGPFFRTSPGQLEAALPAESRERALLERWRELALARDWPGLCMALERESGVH
                     EGLRRAGTG"
     assembly_gap    4190..4199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4200..>5176
                     /locus_tag="PLD82_04260"
     CDS             <4200..>5176
                     /locus_tag="PLD82_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG64633.1"
                     /translation="RDDERRSANLRQILSGVLLAPDAGVHGLEDIAALVRRRYKQAEG
                     LAEDEDIQPLESDGGKVRIMTMHAAKGLQFPVVFIAGGFSEFPKSTKYFEYYNENGKK
                     VFDHLKTGEGKTRAHGYWQGEMQRLFYVALTRAMLRLNIPIVTDKSLSPDITLATLAG
                     LPLRAALESCRERDIPGIVFGDSGTEAVHQKQEQEGGTSSQGDRAALARFERPLPEKN
                     SFKELMVDSFTGLAAVRHTLQTEPDPDATDPVDGETCDGEIAGYKMDDEKMEELPEAE
                     IDPPELDGLICLPRGVESGTLVHGLFEQVDFGMACRDRECRREGVQSIL"
BASE COUNT          990 a         1549 c         1662 g          965 t
ORIGIN      
        1 gtgaacggct ctataccctc ttgacgaatc cggccatcct ggccgggtca ggactggcac
       61 ggaatgctat cgagacctgg ttccaggccc tggccgagct ggggagtttc aggggagaga
      121 ccggtctcga gggcaagcca gactcgttca cctttgccaa gggcctgacc cggctccggt
      181 tggtccgtgt catgggcgat cagccctggc tggatctcgt gccatccggg gcctgcgatc
      241 ccgaactggc tggccgggtg atggtgttag tggaacagct cgagagtacc atccggcagc
      301 ttgaaggaca gagacagacc ggctcccgct ggaggaagac cttgcttgcc ctggccgaaa
      361 cgtttttggg cctgcccggg cagaccatca tggccgaagc gggagcccgt aagcaggccc
      421 tccagcttct gcgtgaactg gactggctgc cggaatgcgg gtttgacacg atcgcggcct
      481 gggccgcggg cagactgggg agcatcaccc tgccgccggc cagggggggc ggggtgatcg
      541 tcgccggtat gcaggatgtc agaccggttc ccttcaggct ggtctatctg ctcggcatgg
      601 gggagggact cttcccgaca ccctggaaac cggcctcgcc cctggacttc ctcgcaaaag
      661 acaatgaccg gtttcccggt gacatcgagg acgccgaacg ggaccggatg ctctttgtcg
      721 agaccctcct cgcggttcgc cagaaactgg tcatcagcta tattgaccgg gacattctca
      781 aggacagtac gcgctatccc tcctccctca tcagcgagtt gcgccagctc gccgacattc
      841 cggacgaggc attcctgtcg attcccgtca ccgggtgggc ggtgacgccc gaggctacgg
      901 cactggatgc gcgtgctccc gacatcatga gcgactacat ccggctcaat gccgggatgg
      961 cccgtctccg ggtgcacccc gaagaggccg aggcggcgag gattgccgcc gtgacttctg
     1021 aacgggaaga acaaaaaagt attgatcttt cttctataaa aagagtgatt gaaaatccgt
     1081 cagccggcat tctcagatat gcctatcggg tcgaaaaaac agctggggaa gccctgtctc
     1141 tcgaggccga cgagccattc attgagacgg atgcaggaaa cgagtccaat ctctggacac
     1201 tggcctccga tctcacgatc ggctggctcc tcgccgaggg ggaggagcct ttcggggact
     1261 tcctcaaggc ctgcgggcgg gagcgtttta tcagcgggta tcaacgctgt gacagggtct
     1321 cggccgggtt tttcggggag ctcatctggc agaaaaagga aaaagacctc cttggacagg
     1381 ccgagggtct gggccaaatc cgcacgcggc tgcgggcggc gctccgtgcc acaaacccgc
     1441 tctggtcgcc cttcgtcttt ggtccggcgc cgacagccgc ccgcgagatc gtccgggggg
     1501 atcttctgcc tggggtccgc ttgcgggaga acctggtcct cggagggata cttcccctgg
     1561 tctggcttga ggatggcggg aaacgcatct gtctgtttgt ggaccggaaa ttcggagcac
     1621 aaaaagtggc taatacgtca gtgctgaatc tgatcccgca gctcgtggtc gccctgcctt
     1681 cggtccggca ggcactcggg ctggacgggc aggccgaggt tcttctggtc tcatacgagg
     1741 cgcctggcgg gattcccctc tttcccggga cagaacagga ggcctgcctg cagcgggtcc
     1801 tcgatcttct tgaacccggg accgcagagc aggcccctgc catgcttcta cccgcgaaaa
     1861 aaattctcgc tgacgacaaa cgcattccgg atgcggaggg cttgctcgtg gcactccaga
     1921 acgctgaaga gaacagccat gacagatttg tcctgaaccc ggcccaagtg ctggtccagc
     1981 ctgtcgtgcc cgatacggac gggatcaaga catggtgtac aagcatgctg gcaccctgga
     2041 tcaggatgct caagccggac tgggtgatcc ccacaagcga ggacgatgat gaatgagaga
     2101 cctgaggcaa aacttatgca atgtcctgag gatgtggacc tcacgcggca tgccgtaatc
     2161 gaggcctcgg ccgggaccgg caagacctgg accattgagc agcttgtcgc ccggatggtg
     2221 agcggggacg gcgattcggc caagggactg gatctcaggc agatcctcgt ggtgaccttt
     2281 accgagcggg ccgccggaga gatgcgtgac cggatccggg ccaccctcgt ggatctggca
     2341 aaagacaagc cggagttgcg ggcctggctc gagtcctgcc tgtcagcctt tgacgaagcg
     2401 atgatcacga ccatccacgg gttctgccgg cgggtgctgt ccgagtatgc cctcgaatgc
     2461 gggctccccg ggtcaccgga actggtcgat gacacggcgg tccactcggc catgctccgt
     2521 cggatctacc gcgactggct ggctgacccc gggcaggagg tcttggcccg cctggccgga
     2581 ttttccgacg atcccgaacg gtaccaaaaa aatctgctca ggcttgccgg gctggaggac
     2641 gtcgttccac ccggcgaagg acccgtcaca agctatgacg aactgccggg tttattggag
     2701 ctgattaaca aagaatttgt gtatgccatg caactgctca agcccttcga ggacgagata
     2761 gaccggcttt gtcaggtgaa aacacttgca ggtcatcatg gaacatatta tacgagaatc
     2821 cgaacaagtt ggctcgtcat ccgcgagtgt ctcgcagtgg atctcgaaag actttctcga
     2881 caggatctcg cagtgtgcat gctgcagatc cactctgcag tggttgaaaa aggcagtgac
     2941 ttcaaagggt atcaaaaaca gtatcatgac ttgaccggag cctgtgttct gaaggtgaaa
     3001 ttaaccattg ccgattgtgc tccggatgcc tgctggccgg gaatcatccc ctgcctggac
     3061 gaggtgatcg accggctcga gacggtccgc aaggggctgg cagagatccg gcgctcgcat
     3121 ctgtatgccg tggccgagcg ggtgcgccgg atggcgcggg ccgaaaaggc cgcccgggac
     3181 cagtacagct acaacgacat gatccgcctg gtccgacacg cggtcgagag gccgggcagg
     3241 ggccttacag ctcgcctcag ggagcgctac cgcgtcgcca tgatcgacga gttccaggat
     3301 acggatctcg cccagtggga aatatttcgt caggtctttg ccgaaagcga cgaaggccat
     3361 accctctgcc tgatcggcga ccccaagcag gccatctacg gattccgggg cggggatgtg
     3421 gcgacctatt tttcggcccg gtcctatctg gtcgagcgca agggggcccg cgtctacacc
     3481 ctcggcacaa actggcgttc gaacacagcc atggtccagg cctgcaatgc cctgtttgcg
     3541 tccacggatg acggcccggt ctggtttttg ccccatcccg ggagtggaga ggcccccgac
     3601 ttcctgccta gtgacgtccc caagcaaccg cgcaacgact gggccgggcc ggcatcgccc
     3661 gacccgatcg tcgtccagga gattggccgc gggaatgcgg ccgccaaccg ctttgccgca
     3721 gccggttgga tcgccgacag gatcggtgcc attcttcgcg gagccctgcc cgaggtccgc
     3781 ctcgcaggaa caaaggaagc ccgtcccctt ggcgccggtg atatcgccat ccttgtccgg
     3841 gccaaaggcg aggccgagcc ggtcctgcgt gtccttcgtg cccggcggat tccctgtacc
     3901 ttctacaagg ccggcggact ctggcgttcg cccgaggccc tggccgtcgt gaccctcttt
     3961 gatgctctgg ccctgggcac gccccaggcc atccgcctgg ccctgctcgg cccgtttttc
     4021 cgcacttcac ccggccagct tgaggctgca ctcccggccg agagccggga gcgcgccctg
     4081 ctcgaacgct ggcgggagct cgccctggcc cgggactggc cgggcctctg tatggccctt
     4141 gagcgtgaga gcggagtcca tgaaggcttg cggcgggcgg gcacaggcgn nnnnnnnnna
     4201 gcgcgacgat gagcgacgga gcgccaacct gcgccagatc ctctccggcg ttctccttgc
     4261 tccggacgcc ggggtacacg gactggagga tatcgccgcg ctggtccgcc ggcgctacaa
     4321 gcaggccgaa ggtttggccg aggacgagga tatccagccc ctcgagagcg acgggggcaa
     4381 ggtgcggatc atgacgatgc atgccgccaa gggactccag ttccctgtgg tctttattgc
     4441 cggcgggttt tcggaattcc cgaagagtac aaagtatttc gaatattaca atgaaaatgg
     4501 caaaaaggtc tttgaccatc tcaaaacggg tgagggcaag acgagggctc atggctactg
     4561 gcagggcgag atgcagcgtt tgttctacgt cgcgctgacc cgggccatgc tccggctcaa
     4621 catcccgatt gtgacggaca agagcctgag tccggatatc acgctggcca ccctggccgg
     4681 cttgccgctc cgtgccgcgc tggaatcttg ccgcgaacgg gacatccccg ggatcgtctt
     4741 cggcgacagc gggactgagg ctgttcatca aaaacaggaa caggaggggg ggacatcctc
     4801 acaaggggac agggctgccc tggcccggtt cgagcgtccc ttgccagaaa aaaactcgtt
     4861 caaggaattg atggtggatt cctttacggg gctggccgcc gtgcgccaca ccctgcagac
     4921 cgagcctgac ccggacgcga ccgatcccgt ggatggagag acgtgtgacg gggagatcgc
     4981 cggatataaa atggacgatg aaaagatgga agagcttccg gaggccgaaa tcgatccccc
     5041 tgaactggat ggtctgatct gtcttccgcg cggagtcgaa agcggaaccc tcgtgcacgg
     5101 gctctttgag caggtcgatt tcgggatggc gtgtcgggac cgggaatgcc ggagggaggg
     5161 tgtgcagtcg atcctg
//
LOCUS       DAOSHE010000108         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14456_length_5126_cov_3.900217, whole genome shotgun sequence.
ACCESSION   DAOSHE010000108 DAOSHE010000000
VERSION     DAOSHE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14456_length_5126_cov_3.900217"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLD82_04265"
     CDS             <1..212
                     /locus_tag="PLD82_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG64634.1"
                     /translation="GVLCGGAVERINDCPDITELVATNSVATPDKAGDKIKVLSCSQV
                     LAKAIQHIHHGDSLTPIFSEYYYNS"
     gene            complement(375..2027)
                     /locus_tag="PLD82_04270"
     CDS             complement(375..2027)
                     /locus_tag="PLD82_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOG64635.1"
                     /translation="MKIRFAGQPPIEMEPGKTVLEIIRESGCHLPEWTVAVRRDNRPC
                     SLSTVLSEDCELVPLTMHSIEGRRIYQKSASLVLLKAFYDVFPGKRIRLLHSLAKGIY
                     CKALLGRALTEEDVARIRERMRELIALDIPFEEESLSREEALSYYESIGLVDKAELLR
                     HLNQDTVRLSRINKVRDLWYFPLVPSTGWLHAWDLVLHKPGLILRYPDTTDPSRLPDL
                     KEQIKLFSVLSEDSEWSKILEVTNVGELNRAISDHSISEVIKIAEALHEKKIARIADE
                     IASRKGQIKFILIAGPSSSGKTTFAKRLAIQLRVNGIKTASISTDDYFLPRDRTPRLP
                     NGDYNFEDIIALDLDLFNTHLSDLFNGKPIMMPKFSFAAGCAIPEKATRLELPRDMPV
                     IIEGIHCLNQELSRSIQRENKYLIYVSALTQLNIDDFNRIPTTDNRLLRRIVRDSLFR
                     NYSALDTLRRWPEVRAGEERNIFPFQEQADIMFNSAQIYELAVLKNFALPRLLQIPPN
                     EPEYGEARRLSNFLTSFLGIEPDEIPPTSILREFIGNSSFHY"
     gene            complement(2336..3193)
                     /locus_tag="PLD82_04275"
     CDS             complement(2336..3193)
                     /locus_tag="PLD82_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG64636.1"
                     /translation="MKSMYIRCTGFCALLALLCSLALSGSPGQITQLTVTVYKAGHGD
                     MIGILSPSGETTLIDCGDSSSTQARAAIRNLPARLARFIATHAHDDHIGNVPLIIGRT
                     TEFYDPGLEHPSRAQEFLYRVVSTGKRLEILSRGAVLDLGGGAVLRVLWPPCPLLRGT
                     AADANNNSLVLMLHFRNFRMLLTGDIETEALEEFMKLDPDLACTAVKIPHHGSRGSLH
                     SGFIRSLKARWAIVSCAKDGDPSGHGLPHEDCLRVWRQAGMAVHATGREGGIRIETDG
                     ETHRLVVLP"
     gene            complement(3255..4226)
                     /locus_tag="PLD82_04280"
     CDS             complement(3255..4226)
                     /locus_tag="PLD82_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64637.1"
                     /translation="MRMLDTRAIDTHDRIMILAQHTARPKLLLCGFFAGVGLDMILGG
                     LLLLLHDLKPGSLPFLLDTPEAILFAVVIALFGATHAWISTTIFALEWSRQRGAGHFR
                     MNFRFVESIFPLVSFALSATLLFLTRLVPEQDCAASIILHIVMAIFMAILMEEFWGYI
                     FRHVLVLLSPVDWRLAVDKERFTQALRTRFDESRRYGIPLSLLVIGIDDFAVLKRSLS
                     RRAITRLQEELIRHIDSAMRTVDSVARLESGQCIGILLHAPEEGTTIAADRLLEQLTG
                     LSWRASRSQSLTPRLVRSIASWSPAMQHERELYELALKGLPESVFRT"
     gene            4539..>5126
                     /locus_tag="PLD82_04285"
     CDS             4539..>5126
                     /locus_tag="PLD82_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64638.1"
                     /translation="MAPRVTKTGKKRRRIESGYLLILSGLVFLGVAALLVIDSRDRVR
                     GPERTAHTRPETVGSGIPAPEEDARAMASRIGTIPGADTRTLDTLVRPVLLEIALRDG
                     QGGFLRDLERVCVDAGWKILSRGDLGDARRLRFSLGRNEPELVLVLHLPARSQSLAHK
                     GPNRVVSEGRSNRRPRIALVLDDAGGEAALQWLFLE"
BASE COUNT         1064 a         1349 c         1668 g         1045 t
ORIGIN      
        1 atggtgtcct ctgcggaggc gctgtcgaac ggatcaatga ttgcccggat atcaccgaac
       61 ttgtcgccac aaactcggtg gccacgcccg acaaggccgg tgacaagatc aaggtgttgt
      121 cttgttcaca ggttctggcc aaagccatcc agcacatcca tcacggcgat tctctcaccc
      181 cgatcttctc cgagtattac tacaacagtt gagggttgtc ctgatcacgt cccgctttcg
      241 cgtgcaggct gcgtcagcca tgcgccacgg acagtcagcc tgtcgcattt tttttactgg
      301 cagcgtggga caccccggtc atcttcaaaa aacattgccg tcttgtgatt gatcagggct
      361 gtgccatgcg ctcttcagta gtggaatgag ctgtttccga tgaactcgcg caggatcgag
      421 gtcggtggga tctcgtccgg ctcaatcccg aggaaactcg tcaaaaaatt cgacagacgt
      481 cgtgcttctc cgtattcggg ttcgtttggt ggaatctgca acagacgagg cagggcgaaa
      541 ttcttcagga cggccagctc gtagatctgg gccgagttga acatgatgtc cgcctgctcc
      601 tggaagggaa agatgttccg ttcttctccg gcacgcacct cgggccagcg ccggagtgtg
      661 tccagggccg agtaattccg gaagagactg tcgcggacaa tccggcgcaa gagcctgttg
      721 tcggttgtcg gaattcggtt gaaatcgtcg atattgagct gggtgagggc actgacatag
      781 atcaggtatt tgttctcacg ctggatcgag cgggagagtt cctggttgag gcagtggatg
      841 ccctcgatga tgacgggcat gtcgcgggga agctcgagcc gggtcgcttt ttctgggatg
      901 gcgcagccgg ccgcaaagga gaacttgggc atcatgatcg gtttgccgtt gaagagatcg
      961 gagaggtgcg tattgaaaag atcaaggtca agcgcgatga tgtcctcgaa attgtaatct
     1021 ccgttgggca ggcgcggggt gcggtccctg ggcagaaaat agtcatctgt cgaaatgctg
     1081 gccgtcttga tcccgttgac ccggagctgg atggcgaggc gtttggcaaa ggttgttttc
     1141 cccgaggaag agggaccggc gatcaggata aatttgatct gacccttgcg tgatgcgatt
     1201 tcgtcggcga tgcgggcgat tttcttttca tgaagggctt cggcgatctt gatcacttcg
     1261 gagatcgagt ggtcgctgat ggcacggttc agctccccca cattggttac ttcgaggatc
     1321 ttggaccatt cgctgtcttc cgagaggacc gaaaagagct tgatctgttc tttgagatcc
     1381 ggcaatcgcg agggatcggt tgtgtccgga tagcgcagaa tcagacccgg tttgtgcagg
     1441 accaggtccc aggcatggag ccagccggtg gatggaacca gaggaaagta ccagagatca
     1501 cgcaccttgt tgatccggga gagtctgacg gtgtcctggt tgagatggcg gaggagttcc
     1561 gccttgtcca cgagaccgat gctctcgtag taagagaggg cttcctcccg ggagagggat
     1621 tcttcctcaa agggaatgtc gagagcgatc agttcccgca tccgttcccg gatccgggcc
     1681 acgtcctctt cggtcaaggc gcggccgagg agtgccttgc aatagatgcc cttggccagg
     1741 gagtgcagga ggcggatccg tttgccggga aagacatcgt aaaaggcctt gagcaggaca
     1801 agggatgcgc tcttttggta gatcctgcgg ccttcgatgg agtgcatggt cagtggaaca
     1861 agctcgcagt cctcactgag gacggtcgag agtgagcagg gacggttgtc gcgacggacg
     1921 gcgaccgtcc attccgggag gtggcatcca gattcccgta tgatctccag aacggttttt
     1981 cccggttcca tctcgatcgg tggctgtcca gcaaaacgga tcttcatgca gatctccatc
     2041 ggtgttgaaa tgagagagac agtctatcag agagtagtgt ccggaagcaa gcagaaagac
     2101 gcaagcacac agatccgctc agagagaacc ggaagcgatg cttcctgagc cgaagcgttc
     2161 aagggaggga ctcagtggag aatccgatca catatatttc ggacgcgcca atgccctgtt
     2221 catccgggta cggacacgaa tatcctgcac aagggtgccg tgtccgtgaa gcaagagcaa
     2281 caggtgaggg aacggctgcc ggccatcgcg gacttcactc cggggacagg cttgttcacg
     2341 ggagcacgac gagtctgtgc gtctctccat ccgtttcgat ccggatgcca ccctccctcc
     2401 cggtggcgtg gacagccatc ccggcttgtc gccagacacg gagacagtct tcgtggggga
     2461 gcccatggcc ggaaggatcc ccgtctttgg cgcagctgac aatcgcccag cgggccttga
     2521 gactccggat aaagccggaa tggagtgaac cccggcttcc atggtgggga atcttgacgg
     2581 ccgtacacgc cagatccgga tccagtttca tgaattcctc cagcgcttcg gtctcaatgt
     2641 cacccgtcaa gagcatcctg aaattcctga agtggagcat caggaccagg gaattgttgt
     2701 tggcatccgc ggctgtcccc cgcaacaggg gacagggtgg ccagagtacc cgcaggacgg
     2761 ccccaccgcc aagatcaagg acggcacctc gggagaggat ttcaagccgc ttccccgtcg
     2821 atacgacacg gtagaggaat tcctgggcac gggagggatg ctccagtccg ggatcgtaga
     2881 actccgtggt ccgaccgatg atcagcggga cgttcccgat gtggtcgtca tgggcgtgtg
     2941 tagcaatgaa tcgtgccagc cttgccggca ggttgcggat ggccgcccgt gcctgggtcg
     3001 aggaggaatc gccgcaatcg atcagggttg tttcaccgct tggagagagg atcccgatca
     3061 tgtcgccgtg ccctgccttg tagacggtga cggtcagctg cgtgatctgt ccgggactcc
     3121 cggagagggc aagagagcag agcagggcaa gcagcgcgca gaaccctgta cagcggatat
     3181 acatggactt cattggtttc ctccttgagg ccgtttacct atgacaacgg gcggcccccg
     3241 ggaggatttt gcattcaagt gcgaaaaacc gattccggta ggcccttgag ggccagttca
     3301 tacagttcgc gttcgtgttg catggccggt gaccatgagg caatgctccg gacaagccgc
     3361 ggggtcagac tctggctccg gctggcccgc caggagagcc cggtcaactg ttcaagaagc
     3421 ctgtccgcgg caatggtcgt accctcttcg ggtgcatgga ggagaatccc gatacactgg
     3481 ccggattcga gtctggccac agagtcgacg gtgcgcatcg cagagtcgat atggcggatc
     3541 aattcctcct ggagccgggt gatggcccgc cgggagaggg accgtttgag gacggcaaaa
     3601 tcgtcgatcc cgatgacaag caaggagagg ggaatcccgt agcggcgtga ttcgtcaaaa
     3661 cgggtccgga gggcctgggt gaagcgctcc ttgtcgacag ccagtctcca gtccacgggc
     3721 gagagcagga ccagaacatg gcggaagatg tatccccaga actcttccat gaggattgcc
     3781 atgaaaatgg ccatcacgat gtggaggatg atggaggcgg cgcagtcctg ctccggaacg
     3841 aggcgcgtca aaaagagcag ggtcgcggag agggcaaaac tgacgagggg aaagatcgat
     3901 tcgacaaagc ggaaattcat tcgaaagtgc ccggccccac gctggcggga ccactccagg
     3961 gcaaagatgg tggtgctgat ccaggcatgg gtggcgccga agagcgcgat caccacagcg
     4021 aagaggatcg cctcgggtgt gtcgagcaaa aacgggaggc ttcccggttt gaggtcgtgc
     4081 aggagcagga gcaagccgcc caggatcatg tccagaccca cgccggcaaa aaagccgcag
     4141 agcaggagtt ttggccgggc ggtgtgttgg gccagaatca tgatccggtc atgggtatca
     4201 atcgcccgtg tgtcgagcat gcgcatggaa gcctccaagg acgtatcgcg gaacgcagtg
     4261 agaagtatag cagatttccg ttgatttcac aatcccggac tcgaaatgcg gaccggatgg
     4321 atcagaaagg tgacgggctt gcgttgccgg tttttttttg atgaacatcc tcaggcccgt
     4381 gatgggcggt ttttccgttg tggacactgg ctgcgggcgg catccggtac gattgctcct
     4441 tgtacgacaa gattgcgccg gactgttccg ggaagagtct ggtgcatttt cggttgaggc
     4501 gacttgacag atgtgccttg cgagccgata ggggagatat ggccccacga gtcacaaaaa
     4561 ccggcaaaaa gcggagacgg atcgaatccg gatacctcct gatcctgtcg ggtctggtct
     4621 tcctgggtgt tgccgctctg ctcgtcatcg acagccgtga tcgtgtccgg ggaccggaac
     4681 ggacagcgca tacgcgtccc gaaaccgtcg ggagtggcat tcccgctccc gaagaagacg
     4741 cccgggcgat ggcctcccgg atcggaacca ttcccggagc ggataccagg acgctggata
     4801 ctctggtccg gccggtcctg ttggagattg cgctgcggga cgggcagggt gggttcctgc
     4861 gggacctgga gcgggtctgt gttgatgcgg ggtggaagat cctttcccgg ggcgatctcg
     4921 gagatgcccg ccggctgcgg ttcagcctgg ggaggaatga gcccgaattg gtcttggtgt
     4981 tgcatctgcc ggcaagatcg cagagtctgg cgcacaaagg accgaacagg gtcgtttccg
     5041 aggggcgttc gaaccggcgc ccgaggatcg cgctcgtcct ggatgatgcc ggcggcgagg
     5101 ccgccctgca atggctcttt ctggaa
//
LOCUS       DAOSHE010000109         5032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14838_length_5032_cov_4.044203, whole genome shotgun sequence.
ACCESSION   DAOSHE010000109 DAOSHE010000000
VERSION     DAOSHE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14838_length_5032_cov_4.044203"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLD82_04290"
     CDS             <1..294
                     /locus_tag="PLD82_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64639.1"
                     /translation="EKFSRRFLGRAHVKGRAEPLAVFELLDAAWPAEIRAARLAAAPH
                     IERAIIARASGDLSEARRRLEEAERCSPKDPAIRRILEELPELSNINSGDNRP"
     gene            complement(269..781)
                     /locus_tag="PLD82_04295"
     CDS             complement(269..781)
                     /locus_tag="PLD82_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG64640.1"
                     /translation="MRGLVVYASHYGTTRQYAVWIATELGFECIEAVKGIPDPSGYDC
                     IIMGGSVYAGGWLLRKWVEQHAGILVRKRVYGFTVSTTPPEKTGEHDAVVLKNLPKNL
                     QEGTPFWHFGGAIDVGKLRFFHRILFRMVMSMSKSPDAGEHKRNGVDYVSRGSILPLL
                     ERVKDDCRQN"
     gene            977..2269
                     /locus_tag="PLD82_04300"
     CDS             977..2269
                     /locus_tag="PLD82_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG64641.1"
                     /translation="MTRTFALAVFCQLALATSLTAATTTLTVKSSQQATSYPPVWIEK
                     GAQIRLDVSGSWSMWHPNWAPVDWRGHTFFNPGSGQHLGRLMASVEGGQPFPVTSGSV
                     WTSTASGYLVLYPHRAKYAHLATTGTLTVRVSGYTHKPLVLSSEAKRVLEQYRNVYLE
                     STITSLGWTGSISGCNAGTLPRSVLDKVLARINYFRSLTGLPPVRFTSERNTKAQHAA
                     LIMLANKQLSHYPDANWRCFSQAGKEGAGSSNLGLTTLDRIITEFIEDPGSNNAPCGH
                     RRWVLYSGASEFGFGAAHDKSHYTFAAEALHVSGNRQAISNLPEFIAWPPKGYIPDPL
                     FFPRGSFSIPKPASAVKLAGARVRMQGSDGSSITVRLNPVSQYGDPGITWEILPANEN
                     LEGFRLKWRNKPIHVFIDGITVDGAPKSWQYTTTMLAP"
     gene            complement(2298..4880)
                     /locus_tag="PLD82_04305"
     CDS             complement(2298..4880)
                     /locus_tag="PLD82_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64642.1"
                     /translation="MRSSSLRRRFIAVIAITCIGALGVSLLGAGILSKRLHREMIAQN
                     SIFARTIAVMVERVLQEPFSVLQQLTIALGIDPAWWSERIETRLRIWSYFESIRVLDQ
                     NGKIIMVVPPDPALFGNDCSQEESFRIPWQSGMPWWSAVTISHPKGSPVMTISIPSGV
                     GVVVGVVHLDGLARYLDTMHTIMGGHVSIVDRTGSYIVHRDAVKVRQRESATRFLHAR
                     QTGGSGPGIVQYKDTENGTNLLVLAQPLSKGGWTVLVSRTEDLVNAPYSVILNLAVPF
                     LVVFVIVVWFVLRRIFKDVLEAIAAFTSEASLLSVGAHSGGRVSVPIEEFSGLAGIFN
                     EMQMRIHQRTGEIQESRERYRILVELLPEAVIVVRQDVFVYANPAAIRVFGAGQTGVL
                     AGMSLDTFLPPEGLGRYSPFTAGTDGVSAPLKASVSLHRLDGSRFEAEVSSITLEYEG
                     QLSVLAIINDISERRRIERELDAMNHRFRNAVESMEDGLWDWNLHDGSHTVSGRYCQI
                     LGFTEEEIPRAHGVLDWKALVHPDDRNMVAETVAGFMAHPEQGYRCEMRMRHRDGRWL
                     EILSRGRVVEKDSIGRATRIMGTIMDMTPVRQAEKERRRLESQLRQEQKLSSVGTLAN
                     GVAHEINNPLMGMINYAQLLQDRLPAGEETWHLWSSEIIHEGMRIAEIVRNLLTFSRQ
                     SDVRRIPVAPGEVVGGILSLSRKLLEKDEIQTDVHFDPDLPAVCCSSAQIQQVILNLL
                     TNARDALNERFPGYDKDKKITMHVTRVCDEEGNWVRIRVADRGCGIPSGILDRVFDPF
                     YTSKPTGKGTGLGLSISYGIVKEHGGRLSYEKNEPDGAAFSIDLPVLADGQSCGEGTA
                     PQPA"
BASE COUNT         1190 a         1589 c         1237 g         1016 t
ORIGIN      
        1 gaaaaatttt cccgacgctt tcttggaagg gcgcacgtca agggccgcgc tgaacctctg
       61 gccgtatttg aattactgga tgctgcctgg cctgcagaga tccgtgcggc ccgtctggct
      121 gcggcacccc atatcgagcg ggcaatcatt gcgagggcaa gcggcgatct cagtgaggct
      181 cgccgcaggc tggaagaggc ggaacgctgt tctccaaaag acccggccat ccgacgcatc
      241 ctggaagaac tccccgaact cagcaatatc aattctggcg acaatcgtcc ttgaccctct
      301 caagaagagg cagaatggat ccccggctga cataatcaac cccgttccgc ttatgctctc
      361 cggcatcggg cgacttggac atgctcatca ccatacggaa caaaatccgg tgaaaaaacc
      421 ggagtttgcc gacgtcaatc gccccgccaa aatgccaaaa cggcgtcccc tcctgaaggt
      481 tttttggcag attcttcagc acaaccgcat catgctcccc tgtcttttcc ggcggagtcg
      541 tcgaaaccgt aaacccgtag acacgcttcc tgacgagaat tccggcatgt tgttcaaccc
      601 atttccgcaa aagccagccc ccggcataga ctgaacctcc catgatgatg caatcatacc
      661 cggacggatc aggaatcccc ttgaccgcct cgatgcattc aaatccaagc tcggttgcaa
      721 tccagacagc gtactgccgt gttgtgccat agtgactggc gtagaccact agtcccctca
      781 tctcgaaacc cctctcgact cctcactgag tctcatcctg cgaagaacag tgattcttgt
      841 acagcaagaa tgataccgtg aagatacttc aattttcgga atatgcctgt caaatcatca
      901 caaaactcgc tggaatctcc cggaaacccg gacagaaaaa agtgtcttac cgggatatca
      961 ctgcatggag ccaccaatga ccaggacttt cgcacttgcc gttttttgcc agcttgccct
     1021 tgcgacaagc ctcaccgcag caacaaccac cctgacggtc aaatcctctc aacaagcgac
     1081 cagctatccc cccgtctgga tcgaaaaagg cgcccaaatc cgtctggatg tcagcggaag
     1141 ctggagcatg tggcacccaa attgggcccc ggttgattgg cgtggtcata cctttttcaa
     1201 tcccggttcc ggacaacacc ttggtcgcct gatggcctcg gtcgagggcg ggcaaccttt
     1261 cccggtcacc agcggatccg tctggaccag cactgccagt ggctacctcg tcttgtatcc
     1321 ccaccgggcc aaatatgcgc accttgcaac aaccggaacg ctcaccgtac gggtcagcgg
     1381 atacacccac aaacctcttg tcctctcaag cgaagcgaag cgcgttctgg aacaatacag
     1441 aaacgtgtat ctcgaaagca ccatcacgag tctgggctgg accggcagca tctcgggatg
     1501 caatgcagga acactccccc ggtcggtgct cgacaaggtt ctggccagaa tcaattattt
     1561 ccggtcgctg acaggccttc caccggtccg gtttaccagt gaacgcaaca ccaaggccca
     1621 gcatgcagca ctgattatgc tggccaacaa gcagctctcg cactatcccg acgccaattg
     1681 gcgctgcttc agtcaagcgg gcaaagaggg cgccggatcc tccaacctcg gcctgacaac
     1741 actggaccgg atcatcaccg agttcattga ggacccgggc agcaacaatg ccccttgtgg
     1801 ccaccgccgc tgggttctct attctggggc atccgaattc gggtttggtg ctgcccacga
     1861 caagtcgcat tacaccttcg ccgcggaggc cctccatgtc tcgggcaatc gccaagcgat
     1921 ttccaacctg ccggaattca tcgcctggcc acccaagggg tacatcccgg atccgctctt
     1981 ctttccccgc ggttcgttct ctatcccgaa accggccagc gccgtcaagc tggctggcgc
     2041 ccgtgtccga atgcagggca gtgacggaag ctccatcacc gtccgcctca atcccgtcag
     2101 tcaatacgga gacccgggca ttacatggga aattcttcca gccaacgaga atctcgaagg
     2161 cttcaggctc aaatggcgaa acaaacccat tcatgtcttc atcgatggca tcaccgtcga
     2221 tggtgctcca aaaagctggc agtacacgac caccatgctc gctccatagc gcctctcctt
     2281 ccgcaacctg cttgaagtca agcaggttgc ggagccgttc cttctccaca cgattgacca
     2341 tccgccaaaa caggaaggtc aatcgagaaa gcagcaccat ccggttcatt tttctcgtac
     2401 gagagccggc cgccgtgttc cttgacaatg ccataactga tggacaaacc cagccccgtc
     2461 cccttgccgg tcggcttcga cgtataaaag ggatcgaaaa cacgatccag aatcccggac
     2521 ggaataccgc acccccgatc cgcaacccgt atcctgaccc agtttccctc ctcgtcacaa
     2581 acccgggtta catgcattgt gatcttttta tccttgtcat acccggggaa tcgctcattg
     2641 agggcatccc gggcattggt caggaggttg agaatgacct gctggatctg cgccgacgag
     2701 caacagacag cgggaagatc cggatcgaaa tgcacatcag tctgaatttc gtccttttca
     2761 agaagttttc tgctgagaga cagaatcccg cccacaacct ctcccggagc gaccggaatc
     2821 cgccggacat cactctgccg ggagaacgtc agaagattcc gcacaatctc cgcaatccgc
     2881 atgccttcat ggatgatctc agaactccaa agatgccatg tttcttcacc agccggaagc
     2941 cgatcctgca gcaattgcgc gtagttgatc attcccatca gaggattgtt gatctcgtgt
     3001 gcaacgccgt tggccaacgt cccgacggag gagagtttct gttcctgacg aagctgtgat
     3061 tccagacgtc ttctttcttt ctcggcctgc cggaccgggg tcatgtccat gatcgtgccc
     3121 atgatgcgtg tcgctctccc gatcgaatcc ttctcgacaa ctcttccgcg ggaaaggatc
     3181 tccaaccagc gtccatccct gtgccgcatc ctcatttcgc agcgatatcc ctgctcggga
     3241 tgggccatga acccggccac cgtctctgca accatgttcc ggtcatcggg gtgcaccaga
     3301 gccttccagt ccagaacacc atgcgcacgc gggatctctt cctcggtaaa tccgagaatc
     3361 tggcaatagc gtccgctgac cgtatgcgag ccatcgtgga gattccagtc ccagagacca
     3421 tcctccatag attcgaccgc attccggaag cggtggttca tggcatccag ctcccgctca
     3481 atccgcctcc gctcactgat gtcattgatg atcgcgagta cagagagttg cccctcatac
     3541 tccagagtaa ttgaagaaac ctctgcttca aaacgcgatc catcgagacg atggagggaa
     3601 acggaggcct tcaacggagc tgaaaccccg tccgtgccag ccgtgaaagg agaataccgc
     3661 cccagtccct ccgggggcag aaaggtatca agagacatgc cggcgagaac cccggtttga
     3721 cctgcaccaa aaacccggat cgcagcagga ttggcataga caaaaacgtc ctgccggaca
     3781 acaatcaccg cttcgggtaa aagctcgacc agaatccggt accgttccct gctctcctga
     3841 atctcaccag tccgctggtg gatccgcatc tgcatctcgt tgaagatccc ggccagaccg
     3901 ctgaattctt cgatcggaac agacaccctc ccaccggaat gcgcgcccac agacaggaga
     3961 ctggcctcgg acgtaaatgc agcgatcgct tcgaggacat ccttgaaaat ccggcgcaaa
     4021 acgaaccaga caacgatgac aaaaacaacg agaaaaggga cggccagatt cagaatgaca
     4081 ctgtacgggg cgttgaccag atcctcggtt cgtgatacca gaacagtcca gcctccttta
     4141 gaaagcggtt gcgccaggac aagaagattc gtcccgttct cggtatcctt gtactgcaca
     4201 atccccggac cgctcccgcc ggtttgcctt gcgtgaagaa aacgggtcgc actctcacgt
     4261 tgcctgacct tgaccgcatc ccgatgcacg atgtacgagc cggtgcgatc cacaatcgaa
     4321 acatggccac ccatgattgt atgcatggta tccagatagc gcgccagtcc gtccagatgg
     4381 acaacgccaa caacaactcc gaccccagag gggattgaaa tggtcatgac cggagaccct
     4441 ttgggatgcg atattgtgac agccgaccac catggcatcc cgctctgcca cggtatccgg
     4501 aatgactcct cctgcgagca atcattcccg aacaaggctg gatccggggg aacaaccatg
     4561 atgatcttcc cgttctggtc gagaacccgg atgctctcga aatagctcca gatccgcaac
     4621 cgggtctcga tccgctcact ccaccaggcg ggatctattc cgagcgcaat ggtcagttgt
     4681 tgcagaacgg aaaaaggttc ctggagcacc ctctcgacca tgaccgcaat cgttctggca
     4741 aaaatgctgt tttgggcaat catctccctg tgcaggcgct tgctgagaat ccctgccccg
     4801 agcagggaga cgccaagggc cccgatgcag gtgatcgcga tcaccgcaat gaaccgtctt
     4861 ctcagactgg aagagcgcat cagattccct ccgcaaggac aaaggtcccc ttttgtacac
     4921 gcaatcgaaa aaacggacgg gagagaacgc catgttcatc aatgagcaac tggctctgga
     4981 ggccctgata cccggaaatc tcctgcatgc tcattgatcc gtcttcgcga cg
//
LOCUS       DAOSHE010000110         5010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_14919_length_5010_cov_4.394753, whole genome shotgun sequence.
ACCESSION   DAOSHE010000110 DAOSHE010000000
VERSION     DAOSHE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14919_length_5010_cov_4.394753"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLD82_04310"
     CDS             complement(<1..596)
                     /locus_tag="PLD82_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG64643.1"
                     /translation="MLKKELRWFFPEKPDEIPDILKKEAKVAFHGGGTSILRIKSDVI
                     TAMVDLSKLGWNKIRKDGSSVVIGATATFNEVVKSSASTACSFRMLQDALSRAASNSI
                     RNRITVGGSLADFPPWSDLIATLIALDAKVTWLEDGKAEKKASVAEFIEKKLGKDKNL
                     VREITVPEKDLSYSVKRLARTSFEYGMFNLAVSCVVKNG"
     gene            complement(774..1607)
                     /gene="folP"
                     /locus_tag="PLD82_04315"
     CDS             complement(774..1607)
                     /gene="folP"
                     /locus_tag="PLD82_04315"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG64644.1"
                     /translation="MSFPLVMGIINVTPDSFWEGSRRPDVAAAVQAARTMQKAGAALI
                     DVGGESTRPGSRPVSIEEEEARVVPVIRALVDEPGLVVSIDTRHPEVAEAALAAGAAI
                     INDVGGLGDPEMRALAADSGARVILMHMQGQPETMQEAPQYRDVLAEIDGFFSTRLDL
                     AVAAGIRRERVWLDPGIGFGKRLEDNLAIIRGLGRFSRHGCPLVMGLSRKSFIGAVTG
                     RPVEERILGTCLYHYAALTAGAAILRVHDVPEAMETISVWRSLQEEQPGKIPTKAVFS
                     P"
     gene            complement(1671..2423)
                     /locus_tag="PLD82_04320"
     CDS             complement(1671..2423)
                     /locus_tag="PLD82_04320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64645.1"
                     /translation="MVRVFVCLSLLSVQSLLVAGMNDARLALRAGRPEEALRLYAECL
                     ADGRDDAALWYGLARARFALDQLVLAAAACDAALFRDPSHQGAWALRGRVRVAQHRFP
                     EAVRDLRQALDLRPDDAWVCGELARVLGRQGRLEEGYRYAILAAESLPADAGRMLGAA
                     WAALALGRWQDAESWCLRAERAGAGRDRVASIMPRIQMALADGERRAGRIREAGTRLL
                     RIISDWPATPQAATARMQLAALGRLAGSGKDP"
     gene            complement(2500..3543)
                     /locus_tag="PLD82_04325"
     CDS             complement(2500..3543)
                     /locus_tag="PLD82_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64646.1"
                     /translation="MMRKAVLSGMLLVSLSLPLSADIAGDLETVGDAGYPGAFLFNLT
                     GAKALSAGNAWTVLGKDPLSVFFNPSTLIGMKGSAAMLSMDLASQNRVQGVAVVALHQ
                     SGETGPLSAEASSGRSSVWALSGAWSSMKDINAYSDNTGTAGADLENASILGQLTFAS
                     AFSRNAGEGGWGVSLRGISESADGTKGQGLALSAGADFNVILGILRCGFSVNNIGFIS
                     HDGRETTWINPLFSAAFLLRLPGLPTPLVIQLDKVLGTDQDVVLRIATEFRLFSLQGD
                     DEASRAIADMNAMQMPAPEVPTELYLRMGLVEGDIYGGMSLRWKLFDLAYGAGMDPVD
                     GLIRHSVSLEVHF"
     gene            complement(3558..3953)
                     /locus_tag="PLD82_04330"
     CDS             complement(3558..3953)
                     /locus_tag="PLD82_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG64647.1"
                     /translation="MLHLLRRGAPLLLALVLVSCAVDEDEVITTFTYPNPCTQESGAK
                     IVVKYQSASAGALSLLVYVMDRAGDAVVVQRKTANVAAGANTIEIPWDVTNDRGAYLA
                     PGPYILKVTTEGLTNSLESHTAWNRILVK"
     gene            complement(3966..>5010)
                     /locus_tag="PLD82_04335"
     CDS             complement(3966..>5010)
                     /locus_tag="PLD82_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG64648.1"
                     /translation="AAVFSLARMTKNNELIAVINSGMSIYRMTASMAIFGLVFSVFLV
                     WFNDEVVFPAERRANTISDKIRHGENPNNKSVSDVRLWGQSGVFWKAKYYDDRKMELR
                     DVLLLKLRQRIGEKLPAEMALNELVNRAVPVKSVTVRSNAEGGADLVVRSVPDINAVR
                     YLDDVRLLTPARYWLYRVEAASARYNKKRQGWDFQKGRIRYYDGPAERVIPFESRFFP
                     LGEIPFDFERADTRVNGMTTGEARAHISKLEKLGAAHRKELVEYYLKFSFPLVNGIII
                     IIGISFGGFSPRSVLVLSFFIAVLIYLLYYTFVALGLSLGKTGVLDPALGAWLGNIGF
                     FLIGAGLLVFRKT"
BASE COUNT         1084 a         1579 c         1397 g          950 t
ORIGIN      
        1 ccgtttttca caacacaaga gaccgcaagg ttgaacatcc cgtactcgaa actggtgcga
       61 gccagtctct tcacggaata ggagagatcc ttttcgggaa cagtgatctc gcggacgaga
      121 ttcttgtcct tgccgagctt tttctcgatg aactccgcaa cgctggcctt cttttcggct
      181 ttcccgtctt caagccaggt caccttggca tccagtgcga tcagtgtcgc gatgagatcc
      241 gaccagggag gaaaatcggc cagggacccg ccgacggtga tccggttccg gatggaattg
      301 gaagcggccc gtgagagtgc gtcctggagc atgcggaagg agcaggccgt cgatgcgctg
      361 gacttgacaa cctcgttgaa cgttgccgtt gcaccgatca caaccgagga accatccttg
      421 cggatcttgt tccatcccag ttttgaaaga tcaaccatgg ctgtgatgac atcggacttg
      481 atccgcagaa tggacgtacc accgccatga aacgcaacct tcgcttcttt tttcaggatg
      541 tccgggattt cgtctggctt ctccgggaag aaccacctga gttccttctt gagcatatcc
      601 taacctcaca ctgttttttt gacactcgat gacgccacat cccgaaacgg cactgattcc
      661 ggactttagg tggggacaga agaatactgt accaacaaaa aggtcgggac atgacaggct
      721 tgactccatg cacacgaagc cgggcatagt ctatcccttt tcaaggctgt ctgtcaagga
      781 gaaaaaacag cttttgtcgg aatcttgccg ggctgctctt cctgaaggga ccgccatacc
      841 gaaatggtct ccatggcttc aggaacatcg tggaccctga ggatggcagc cccggccgtc
      901 agtgcggcat aatggtacaa acaggttccc aggatgcgct cttcaaccgg ccgccccgtc
      961 acagctccga taaacgactt ccgggaaagg cccatcacca aggggcagcc atgccgggag
     1021 aagcgcccga gcccccgaat gatggccaga ttgtcttcaa gacgctttcc gaacccgatc
     1081 cccggatcaa gccagacccg ttcgcgccgg ataccggcgg ccacggcgag gtccagccgg
     1141 gtgctgaaga aaccgtcgat ctcggccagc acatccctgt actgcggtgc ctcctgcatg
     1201 gtttccggct ggccttgcat atgcatcaga atcacccggg caccggaatc ggctgcaagg
     1261 gcacgcattt ccgggtcgcc cagcccgccg acatcgttga tgattgcggc accggcagcg
     1321 agcgcggctt ccgccacctc gggatgccgg gtgtcgatcg agacaaccag tcccggctcg
     1381 tcgaccaagg cacggatcac ggggacaaca cgcgcctcct cctcttcgat ggacaccggc
     1441 ctggaccccg gtcgggtgga ttctccgccc acgtcgatca gagctgcccc cgccttctgc
     1501 atggtccggg ccgcctggac agccgctgcg acatctggcc tgcgactccc ttcccaaaac
     1561 gaatccggcg tcacattgat gatccccatc accaatggaa atgacatttc cggctcaacc
     1621 tttcccttca aggatggcat cctgttcctc ctccaaaaaa ccggtctggg tcagggatct
     1681 ttcccggatc cggccaatct cccgagtgcc gcaagctgca tcctggcagt cgcagcctgg
     1741 ggagtagcag gccaatcact gatgatccgc agaagacggg tcccggcctc ccggatccgg
     1801 ccggcccggc gctctccatc cgccaaggcc atctggatcc gcggcatgat ggacgcgacc
     1861 cgatcacggc cggctccggc ccgttctgcc cggaggcacc aggactcggc atcctgccag
     1921 cggccgagcg ccagtgctgc ccaggccgcc ccgagcatcc ttccggcatc ggccgggagg
     1981 ctttctgccg ccagaatggc ataccggtag ccctcctcca gcctcccctg tctgccgaga
     2041 accctggcca gttccccaca gacccaggca tcatcgggac ggagatccag cgcctgacgc
     2101 aggtcgcgca cagcctcggg aaagcggtgc tgggcgacac gcactcgccc tcgcagagcc
     2161 caagcccctt gatgggacgg atccctgaac aaggcagcat cacaggccgc tgccgccaag
     2221 accaactggt caagggcaaa gcgcgcccgg gccaggccat accagagagc ggcatcatcg
     2281 cgcccgtcag ccaggcactc cgcgtagagc cggagggctt cctcggggcg tccggcccgg
     2341 agcgccaatc gcgcatcgtt cattccggcg acaagcaggc tctggacaga gagcagggag
     2401 agacaaacga atacacgcac catgaaatcc tcctgacggc gaaccttcat ccagatacga
     2461 aggagaggtc cggagccagt ccgtggtgcg ctgttttttt taaaaatgca cctcaagcga
     2521 gacgctgtgc cggatcagtc cgtcgaccgg atccattccg gctccgtatg ccaggtcaaa
     2581 cagcttccag cggagactca tcccgccgta gatatcccct tcaaccaatc ccatccgcag
     2641 atagagttcg gtcgggacct cgggagccgg catctgcatg gcattcatgt ccgcaatcgc
     2701 cctgctggcc tcgtcatctc cctgaaggga gaagagcctg aattccgtgg caatccgcaa
     2761 gacgacatcc tgatccgttc cgaggacttt gtccagctgg atgaccagcg gtgtgggcag
     2821 tccgggaaga cgcaggagaa aggcggcaga gaaaagcgga ttaatccagg tggtctcccg
     2881 tccgtcgtgc gagatgaagc cgatattgtt gaccgagaaa ccacaccgga gaattccgag
     2941 aatgacattg aaatcggcac cggccgagag agccaatccc tgtcccttgg tcccgtcggc
     3001 actctcgctg atcccgcgca ggctgacgcc ccagccaccc tccccggcat tccggctgaa
     3061 tgccgatgca aaggtcagct gtccgaggat ggatgcattc tcgaggtccg cgccggccgt
     3121 accggtgttg tcactgtagg cgttgatgtc cttcatgctt gaccaggcgc cggagagcgc
     3181 ccaaaccgag gatcgtccgg atgaggcctc ggccgagagc ggtccggtct ccccgctctg
     3241 atggagggcg acaacggcca caccctggac ccggttctgt gaagccagat ccatggaaag
     3301 catggcggca cttcccttca tcccgatcag ggtcgaggga ttgaaaaaaa cggagagggg
     3361 atctttcccg agaaccgtcc aggcatttcc ggcagaaaga gccttggccc cggtcaggtt
     3421 gaacaaaaaa gcaccgggat acccggcatc acccaccgtt tcaagatcac ccgcgatatc
     3481 ggccgacaac gggagggaga gggaaaccag caacatcccg gagagaactg ctttgcgcat
     3541 cattcgtccc ttttctttca tttcaccaga atcctgttcc aggctgtgtg cgactcgaga
     3601 ctgtttgtca ggccctcagt cgtgaccttg aggatgtacg gacctggtgc gagatacgcg
     3661 ccccggtcat tggtcacatc ccacgggatc tcgatcgtat tggctccggc ggccacattt
     3721 gccgtcttgc gctggacgac aaccgcatcc ccggctctgt ccattacgta gacgagaagg
     3781 gagagagccc ccgcagaggc agactggtat ttcacaacga tcttcgcccc gctctcttgc
     3841 gtgcaggggt tgggatacgt gaaggtggtg atcacctcgt cttcatccac cgcacaggat
     3901 acaagaacca gagcgagcaa gagtggcgct ccgcgccgca gaagatgcaa catccagaaa
     3961 ctcctttatg tctttctgaa caccagcaat ccggcgccga tcaggaaaaa accgatattt
     4021 cccagccagg cacccagtgc cggatcgagg acgccggtct tccccaacga cagcccaagg
     4081 gcaacaaaag tataatagag caggtagatc agcactgcaa taaaaaacga gaggaccaga
     4141 accgatctcg gagaaaaccc gccaaaggat atcccgatga tgatgatgat cccgttcaca
     4201 agcggaaatg aaaatttgag atagtattca accaattcct tgcggtgggc ggcccccagc
     4261 ttctcgagct tgctgatgtg ggcacgggcc tccccggtcg tcattccgtt gacccgggta
     4321 tccgcccgtt cgaaatcgaa gggaatctcg cccagcggga agaaccggga ttcgaacggg
     4381 atcacccgct cggccggacc gtcgtaatag cggatcctgc ccttctggaa atcccatccc
     4441 tgccgttttt tgttgtagcg ggcggatgcc gcctcaaccc tgtagagcca atatcgggct
     4501 ggtgtcagga ggcggacatc atccagatag cggacagcat tgatgtcggg tacggaacgc
     4561 accacaagat cggccccgcc ctcggcattg gacctgaccg tgacgctctt gacgggtacg
     4621 gcccggttga ccagttcgtt cagcgccatc tccgccggga gtttttcccc gatccgctgc
     4681 cggagcttga ggaggaggac gtcccgaagt tccatcttgc ggtcatcata gtacttcgcc
     4741 ttccaaaaaa ccccgctctg cccccaaagc ctcacatcag aaacactttt gttgttcgga
     4801 ttctctccgt gccggatctt gtcggagatc gtgtttgccc tgcgttcggc ggggaaaacg
     4861 acctcgtcgt tgaaccagac aaggaagaca ctgaagacca ggccgaaaat ggccatggat
     4921 gccgtcatcc ggtagatgct cattcccgaa ttgatgaccg cgatcagttc gttgttcttg
     4981 gtcatccgcg ccagagaaaa aaccgccgca
//
LOCUS       DAOSHE010000111         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15030_length_4981_cov_3.916565, whole genome shotgun sequence.
ACCESSION   DAOSHE010000111 DAOSHE010000000
VERSION     DAOSHE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_4981_cov_3.916565"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLD82_04340"
     CDS             complement(<1..755)
                     /locus_tag="PLD82_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64649.1"
                     /translation="MIRTLFTMVVLSLLMLVSANCSIGPDVVGVTDDTTVDRAVTYTE
                     DFEKSTASGTSYVTGSFVGNNGLTWNYVQTAWSGAVIAGSKSPVLGMNRTPVAEVSTT
                     VADGVGTLSFKFKKAYSTAVSLSVYANDTLLGTVTTTKTTTQSKTFTVNKSGTVKIRF
                     VQSKTTAGQVSIDTLSWTSYGTTSSSSSVSSSSSSVSSISSSVSSSSSSTAATGTIKI
                     ASFNIQIFGSTKVARANVLYTLAHIATRYDIIA"
     gene            complement(1129..2832)
                     /gene="recN"
                     /locus_tag="PLD82_04345"
     CDS             complement(1129..2832)
                     /gene="recN"
                     /locus_tag="PLD82_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG64650.1"
                     /translation="MLESLIIRNFALIEDLSIDFGRGFNVLSGETGAGKSIIIGALGL
                     ILGGKSSSEMVRTGTDMASIEGLFSVSPDSAAVTMLAGAGIDTPSGELVIRRTVSADG
                     KSRCFINGVLSNMTVLSELGALLVDIHGQHENQSLLKISLHLGMLDEFAGLAGSVDGM
                     RGLVRELARLEEQLSALAMDDREKARRLDLNTHAIREIDAAGLRENEEAELEEENRLL
                     SNFEKIHGAVESACETLAPAAGEVRSAAVGLESAGEHDPRLAGLAQRIRDAWYALDDI
                     TDELRAWRGRIDFSPERLEEVNERISLIQTLKRKYGEGIPEILAFRDKAAEENESIQT
                     SDQRRAELSGEIAALQERVSRAALALSQERQKRAREMETRVMEELAFLGMAKTVFKVD
                     IRYVRDPEGFIEVRGERIRLFETGVDHVEFLVSPNPGEEARPLREIASGGEMSRIMLA
                     FKSVLSSRNDCETLVFDEVDAGIGGVTASAVGKKLRQVSGGHQVLCITHLPQIAAMAD
                     QHIMVSKSVADGRTRTQIRHLAHDERLAELARMLGGEVVSETTLQQAREMIEMGQGGP
                     S"
     gene            complement(2906..3766)
                     /locus_tag="PLD82_04350"
     CDS             complement(2906..3766)
                     /locus_tag="PLD82_04350"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG64651.1"
                     /translation="MKREIRSAGIVRHPHKDCRALTEAITSRLEARGVVVTVVDAGRS
                     EDGAVVRPAIDGMALDLAISLGGDGTLLSTARLVYGRSIPVIAVNMGTFGFLTAISHD
                     EVLRALDALFAGSVGFENRMMLEARVERDSRTVVLCRALNEVVVTRRDMASMIGLAVW
                     VNGEFLSGYRADGLIVATPTGSTGYSLSASGPILMPTLENMIITPVCPHSVASRPFVV
                     GAADTVTIQVRALNLRSALTIDGQEEIPLEDGDEIVVSRAAHPLVLVRSGERSYLEVL
                     RTKLAWSGAP"
     gene            complement(3801..4145)
                     /locus_tag="PLD82_04355"
     CDS             complement(3801..4145)
                     /locus_tag="PLD82_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014487599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG64652.1"
                     /translation="MEELLTKQVDDVLIVYLSGRLDVHLSLEIEQAVNELIDKGHINL
                     LFDLEKVVYLSSSGLRIFIAAMRKLKELNGQLKLANLTSSVKKIFKVVELIDLFDIYD
                     SVDEAMASFKKQ"
     gene            complement(4190..4600)
                     /locus_tag="PLD82_04360"
     CDS             complement(4190..4600)
                     /locus_tag="PLD82_04360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64653.1"
                     /translation="MTEFCVLSKTENVRVIGSWLKEFCEARHVGSEELEDIIIATDEA
                     VTNIMMHGYQGRTDGLIEIRCRVLDRKVELRIRDNGMTFSMPGLDTLVNKKREEYLVK
                     GGYGLILMHRFMDGVEFRQDGQSGKNVLLMHKNF"
     gene            complement(4742..>4981)
                     /locus_tag="PLD82_04365"
     CDS             complement(4742..>4981)
                     /locus_tag="PLD82_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG64654.1"
                     /translation="YRYPLRVKRSLKDAIEFLKKNIIEAKPPVARPLHQILGLPAEAF
                     PETEKAFLKTLSIPIYPALLNKEVEYIAKVVTRVT"
BASE COUNT         1110 a         1521 c         1359 g          991 t
ORIGIN      
        1 atagcaatga tgtcgtagcg ggtggcaatg tgagccaatg tgtagaggac gttggcccgg
       61 gcgaccttgg ttgaaccgaa gatctggata ttgaacgatg cgatcttgat ggtaccggtc
      121 gcggccgtcg aggagcttga actggaaaca gaagaagaga tggaggagac agaagacgaa
      181 ctcgatgaaa cagaggagga gcttgatgtc gtcccgtagc tcgtccagga gagtgtatca
      241 atcgagacct gtccggctgt cgtcttggac tgcacaaagc ggatcttgac cgttccggac
      301 ttgttgaccg taaaggtctt gctctgggtc gttgtcttgg tcgttgtcac cgtccccagg
      361 agcgtatcat tggcatagac gctcagactg accgctgtcg aatacgcctt cttgaacttg
      421 aaagagaggg ttccgacacc atcagcgacc gttgtggaca cttcggcaac aggggtgcgg
      481 ttcatgccca aaaccggtga tttggaaccg gcaatcacgg cgccggacca ggcggtctgg
      541 acgtaattcc aggtcagccc attgtttccg acaaagctgc cggtcacata ggatgtccca
      601 ctggcggtgg atttttcaaa atcctcggta tacgtgacgg cacgatccac ggtggtgtca
      661 tcggtcacgc cgacaacgtc tgggccgatg ctgcagttcg cgcttacaag catcagcaga
      721 gaaagaacaa ccatggtgaa caatgtgcgg atcatattca tctccttgta atattgggag
      781 gaagagcgtg gcatcaccca acccgacggt tcgcgctcgt cggatacaac cactcgtcac
      841 ccccgcgtta cagtaaccat aggttgattc tgcccggtcc gcaatagggt aaaaccccta
      901 aaaattccaa aatttgcgga tttttgttga cggcggattc gcattttcgg aattggcacc
      961 aaagattgac gccaacacac ctagagactc ccgggacaca gcttttgcag gaagattcga
     1021 gcggcacagc agccggaaac ggccgcgagc ggctcgctcc aagatccgcc gcctgacagc
     1081 ggactgagga tgttcatcaa aaaaaaccaa tcatgacaga tccaacagtc aggagggacc
     1141 gccctgtccc atctcgatca tctcccgggc ctgttggagc gtcgtctcgg agacgacctc
     1201 gccacccagc atccgcgcca gttctgccag acgctcatca tgggccagat gccggatctg
     1261 ggtccgcgtt cgaccatcgg ccacactctt cgagaccatg atgtgctggt ccgccatggc
     1321 cgcgatctgg ggaagatggg tgatacagag gacctgatgt ccgccggaga cctgccgcag
     1381 ttttttcccg acggccgaag cggtcacgcc gccgatcccc gcatccacct cgtcaaagac
     1441 cagggtctcg cagtcattac ggctcgagag aacggatttg aaagccagca tgatgcgcga
     1501 catctcgccg cccgaggcga tctcgcgcag gggtcgggcc tcctcgccgg gattgggcga
     1561 gaccagaaac tcgacgtgat ccacacccgt ctcgaaaagc cggatacgct cgccccgcac
     1621 ctcgataaag ccctccggat cgcggacata gcggatatcg actttaaaaa cggtctttgc
     1681 cattcccaaa aacgccaact cctccatgac gcgggtctcc atctcccggg cccgcttctg
     1741 ccgctcctgg gaaagcgcca gggcagcccg cgaaacccgc tcctgcaggg ccgcgatctc
     1801 gccggagagt tctgcccgac gctgatcact ggtctggatg gactcgtttt cctcggcggc
     1861 cttgtcgcgg aaggcgagga tctcagggat accctccccg tatttccgct tgagggtctg
     1921 gatcagggag atccgctcat tgacctcctc cagccgttcg ggtgaaaaat cgatccgtcc
     1981 acgccaggcc cggagttcgt ccgtaatgtc atccagtgca taccaggcat cccggatacg
     2041 ctgggcaagc ccggccagac gcggatcatg ttcacccgcg gattccagtc caaccgctgc
     2101 ggaacggacc tccccggcgg ccggagcgag ggtctcgcag gccgactcga cagccccatg
     2161 aatcttttcg aaattggaga ggaggcgatt ttcctcttcg agctcggcct cctcgttttc
     2221 cctgagaccc gcagcatcga tctcccggat ggcgtgggta ttgagatcca gacgacgcgc
     2281 cttctcccgg tcatccatgg ccagggcgga caattgctcc tcgagtctgg ccagctcccg
     2341 gaccaggcca cgcatcccgt ccacagaacc ggcaagaccg gcaaactcgt ccagcatgcc
     2401 aagatgcagg gagatcttga gcagggactg gttctcgtgc tgtccgtgga tatccaccag
     2461 gagagcgccc agttcggaca gaaccgtcat gttcgacaac acaccattga tgaagcagcg
     2521 cgactttccg tcagcactga cggtccggcg gatgacaagt tcaccggagg gggtgtcgat
     2581 cccagccccg gccagcatcg tgacggcggc cgaatccggc gagaccgaaa acaggccctc
     2641 gatactggcc atgtccgtcc cggtccgtac catctcgctg gaggacttcc ccccaaggat
     2701 cagcccgaga gcaccgatga tgatggattt tccggccccg gtctcgcccg aaagaacatt
     2761 gaacccgcgg ccaaagtcga tggaaagatc ttcgatcagg gcgaagttgc gaataatgag
     2821 ggattccagc atctgcctgc tccttgttgc ctctccagaa taataccgcc cgcctccccc
     2881 gagtattgca tccggggaga aaaaatcagg gagcaccact ccaggccagt tttgtccgca
     2941 gaacctcaag atagctccgt tcaccggacc gcaccaggac caggggatgc gcagccctgg
     3001 agacgacaat ctcgtcaccg tcctccaggg gaatctcttc ctgtccgtca atcgtcaggg
     3061 ccgaacgcag gttcaaggcc cggacctgaa tcgtgaccgt atcggccgcc ccgaccacaa
     3121 aggggcgtga agccacagag tggggacaga caggggtgat gatcatgttt tcaagggtcg
     3181 gcatcaaaat cgggccggag gccgagagag agtacccggt cgaaccagtc ggggtcgcca
     3241 cgatcagccc gtccgcccgg tatccgctca aaaattctcc attgacccag accgccagcc
     3301 cgatcatgct tgccatatcc cggcgggtga cgaccacttc gttgagggcc cggcacaaga
     3361 ccaccgtccg ggaatcccgt tcgacacgcg cctcgagcat catcctgttc tcgaatccaa
     3421 ctgaaccggc aaagagggca tccagtgcgc gcaggacttc atcgtgggag atcgccgtca
     3481 aaaaaccgaa ggtccccata ttgacggcaa tgacgggaat cgaccggcca tagacgagcc
     3541 gagccgtcga gaggagtgtc ccgtcccccc cgagggagat ggccagatca agcgccatcc
     3601 cgtcaattgc gggacgaacc accgccccat cctcactccg gcccgcatcg acaaccgtaa
     3661 caacaacacc ccgcgcctca aggcgcgagg tgatggcttc ggtcaacgca cggcaatcct
     3721 tgtgcggatg gcgcacgatc cccgcactgc gaatctctct cttcattccg cttgtccccg
     3781 gggagaatcc cgcggagaga ttactgcttt ttgaacgagg ccatggcctc atcaacggaa
     3841 tcgtagatat caaaaaggtc gatcagttcg acaactttga agatcttctt cacagatgac
     3901 gtcaggttgg ccagcttgag ctgcccattc agttccttga gtttgcgcat cgcggcgatg
     3961 aaaatccgca acccgcttga cgagaggtag acaacctttt cgagatcgaa gagcagattg
     4021 atatgcccct tgtcgatgag ttcgttgaca gcctgctcga tctcgagcga gagatggaca
     4081 tcgagccggc cactgagata cacgatgagt acatcatcga cctgttttgt aagcaattct
     4141 tccattgtga gaagtccccc agacttttta tagataccac tgtactctgc taaaaattct
     4201 tgtgcatcaa caatacattt tttccgctct gtccgtcctg ccgaaattcg accccgtcca
     4261 tgaagcggtg catgaggatc agcccgtatc cgcccttgac cagatactcc tcgcgctttt
     4321 tgttgaccag ggtatcaaga ccaggcatgc tgaacgtcat accgttatcc ctgatccgca
     4381 actcgacctt gcgatccagg acccggcagc gaatctcgat caagccatcc gtccggccct
     4441 gatacccgtg catcatgatg ttggtcacgg cctcgtccgt cgcgatgatg atatcctcca
     4501 gctcctccga accgacatgg cgggcctcgc aaaactcctt gagccaggaa ccgatcaccc
     4561 ggacgttttc cgtcttgctc agaacacaaa actcggtcat ccctctttgg cgccccatgc
     4621 ccctgcctca accaagtcag acatcactca tcattgtatc cattcagaat catccctgtc
     4681 aagcagcaaa tatgaagaag tcgcgtccgg cggagaaatc cacgcccttc tgcgacacag
     4741 ctcaggtgac tctcgtgacg accttggcga tatactccac ctccttgttg agaagggcgg
     4801 ggtaaatcgg tatcgagagc gtcttcaaaa acgctttttc ggtttcggga aacgcctcgg
     4861 caggcaagcc gagaatctgg tgcaagggcc gggcgacggg cggcttggct tcgatgatgt
     4921 tttttttgag gaactcgatc gcatccttca ggctgcgctt gacccgcagg ggatagcggt
     4981 a
//
LOCUS       DAOSHE010000112         4972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15064_length_4972_cov_3.982916, whole genome shotgun sequence.
ACCESSION   DAOSHE010000112 DAOSHE010000000
VERSION     DAOSHE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15064_length_4972_cov_3.982916"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLD82_04370"
     CDS             complement(<1..1350)
                     /locus_tag="PLD82_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG64655.1"
                     /translation="MQLIEKTIGQVFDACVAAHPDRECLIYADRNLRFTWCQFDRRVK
                     DMAKGLLALGLKKGDHLGIWATNVPDWLTFMYATAKIGVVLVTVNTNYRTHELEYLVR
                     QSDISTLCLIDGFRDNDYVAMVNELIPELKTCERGSLVSVKFPRLKNIIFIGPQKHRG
                     MFSTPELLLLGQQMDDAVLDGIAKELDTNDVINMQYTSGTTGFPKGVMLTHRNILNNG
                     YWIGECQKFTGQERVCLPVPLFHCFGLVLGIMAILTHGATAVVVEAFDPLLVLASVQK
                     ERCTALYGVPTMFIMELNHPMFRMFDTSSLRTGIMAGSPCPVEVMKQVIDEMHCRDIT
                     IAYGLTESSPVMTQTRTDDPIEVRVGTVGRELPGIEVSVRDPESGEECPGGVQGELCC
                     RGYNVMKGYYNMPEATARIIDGNGWLHSGDLGVKDADGNYKVTGRIKDMIIRGGENIY
                     "
     gene            complement(1365..1928)
                     /locus_tag="PLD82_04375"
     CDS             complement(1365..1928)
                     /locus_tag="PLD82_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOG64656.1"
                     /translation="MSRQLGEKIRSVRESRNVTLEQLGEKCGMPADVIAKIESGELVT
                     SLTPLIKIARVLGVRLGTFLDDQESLGPVVTRSGAQAKVARVGGPAAGGGMDFFSLAQ
                     GKTGRHMEPFIIELDPASGGGATPSSHEGEEFIHVLEGAVEIIYGRENFRLETGDSIY
                     YDSIVSHQVRSADGRSAKILAVVHAPL"
     gene            complement(2037..2705)
                     /locus_tag="PLD82_04380"
     CDS             complement(2037..2705)
                     /locus_tag="PLD82_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG64657.1"
                     /translation="MSPGSPWEILGIPGDASRETARKAWLALVRIHHPDAGGDVSRMA
                     EINAAWAMISGGEYRVCQPFRRNAVHSGSVGVQQKEPVREIAKSAPEILASRDGAGAR
                     PEMILDGRGWRAEGLPPWSIRIRFAAPAMVESAVAEALPLPYAIKRAHHRAVELHEIV
                     LRLTDGRELRFPFTLTVCADRDLALRFPDGAGPAESLEIVTHEALLPPGWKNLRIHGR
                     LSGT"
     gene            complement(2702..4288)
                     /locus_tag="PLD82_04385"
     CDS             complement(2702..4288)
                     /locus_tag="PLD82_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64658.1"
                     /translation="MRLSNGSKSFSPVPWCIRIVFFLILAVSATLVASNQPQRAIFPD
                     ILAPLYQPWDFRIRSARITGDLVSLEKQHLWLVKTRPFDLGVRWQLVEFYTQCGMTNK
                     IRTAASEYRSLLARSPAWQWNAWNLWFAAARSHDLSGEEAKALELYRTSLRFGATAHA
                     WNRIMELELAAGRHAAAIEACRRWAAISGFPQGDRERVLAVCFERCGNKEYAQAAYLR
                     SLQANPLDLLSLEGLARTGASNSVLVARAVRTVAESGYNLDYLNNGIETCLTAGRPEA
                     ARFAVRELAGWFRARRSVYWSMAAARADEAAGCYHDAAMHFRQARLWRPGAPPDETAY
                     YLERELRLLTRAERIPEAVTLFNSLGHILSGETRSLIEIGMMRATNADASLIEGALVG
                     LAAGDNGDWRPLTAALLGRIDELFPDAACREVRGRLWRLCAARNQDPVTRYAAGKFFL
                     EQGLPAVAIGYLHRAREGMRTLGQISVDISVAARLLGLKEDEDWHDSRARYLLGHEAA
                     NTGKTVLAQRIRKDLHEAVP"
     gene            4499..4569
                     /locus_tag="PLD82_04390"
     tRNA            4499..4569
                     /locus_tag="PLD82_04390"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Gln,seq:ttg)
     gene            complement(4540..4920)
                     /locus_tag="PLD82_04395"
     CDS             complement(4540..4920)
                     /locus_tag="PLD82_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG64659.1"
                     /translation="MKYEFSLESTMFCGIPVILLQGDITAESDSRIKEAWDVALQYPS
                     EVIAFEFSRTKYVNSAGVATLLSLVTRAVSQSRKVLFVGLTAHFRKVVELIGMTDFVQ
                     VADTMAEYVAGFGPDQPGKDSNPQ"
BASE COUNT         1060 a         1479 c         1413 g         1020 t
ORIGIN      
        1 gtagatgttc tcccctccgc ggatgatcat gtccttgatc ctaccggtta ccttgtagtt
       61 gccgtcggca tccttgaccc cgagatcccc ggaatggagc cagccattac cgtcgatgat
      121 cctggccgtc gcttcaggca tgttgtaata gcccttcatg acgttatagc cgcggcagca
      181 gagttcgccc tggactccgc caggacactc ctcgccgctc tcgggatccc ggacggagac
      241 ctcgataccg gggagttccc gtccgaccgt tccgaccctg acctcgatcg ggtcatccgt
      301 cctggtctgg gtcatgaccg gtgaagactc ggtcaatcca taggctatcg taatatcacg
      361 acaatgcatt tcatcaataa cctgtttcat cacctcgacc gggcagggcg atccggccat
      421 gatcccggtc ctcagactcg aggtgtcgaa catcctgaac atgggatggt tcaattccat
      481 gatgaacatc gtcgggacac catagagtgc cgtacaacgt tctttctgga ccgaagccag
      541 caccagaagc gggtcaaagg cttcgacaac gacggccgtc gcaccatgcg tcaggatcgc
      601 catgatcccc agcaccagac cgaaacagtg gaagagcgga acagggagac agacccgttc
      661 ctgtccggtg aacttctggc actccccgat ccaatacccg ttgttcagga tattgcggtg
      721 ggtcagcatg acacccttgg gaaaaccggt tgttcccgag gtgtactgca tattgatcac
      781 gtcatttgtg tcgagttcct tggcgatccc gtccaacacc gcatcatcca tctgctgtcc
      841 gaggaggagc agctccgggg tcgagaacat ccctctgtgc ttctgcggtc cgatgaagat
      901 gatgtttttc agacgcggga atttgacgct cacaagggat ccgcgttcgc aggtcttgag
      961 ttcggggatc agttcgttga ccatggccac ataatcattg tcccggaagc cgtcgatcag
     1021 acagagtgtc gaaatatcgg actgccggac aagatattcc agctcgtggg tccggtaatt
     1081 tgtattgaca gtgaccagca caacgccgat cttggccgtc gcatacatga aggtcaacca
     1141 gtccggaaca ttggttgccc aaatcccgag atgatcccct tttttcaggc cgagtgcgag
     1201 caagcccttg gccatgtctt tgacccgcct gtcgaactgg caccaggtga agcgcagatt
     1261 ccggtcggca tagatcaggc actccctgtc gggatgggcg gccacacagg cgtcgaagac
     1321 ctggccgatt gtcttttcaa tgagttgcat ggatcgggct cccgtcagag tggtgcatgg
     1381 acaacggcca ggatctttgc cgaccgaccg tcagcactcc ggacctggtg ggacacaatc
     1441 gaatcgtagt agatgctgtc tccggtctcg agccggaagt tttcgcgccc atagatgatc
     1501 tcgacagcac cctcgagaac atggatgaat tcctcaccct cgtgcgagga cggagtcgcc
     1561 ccgcctccgg aggccggatc gagctcgatg atgaagggtt ccatgtgccg tcccgtcttg
     1621 ccctgggcaa gcgagaaaaa atccatcccg cctcctgctg caggtcctcc gacacgggcc
     1681 actttggcct gggctccgga ccttgtcacg accggaccga gcgattcctg gtcatcgaga
     1741 aaagttccca gacgcactcc gagaacccgt gcgattttta tcaggggggt cagggaggta
     1801 accagttccc cgctctcgat ttttgcgatc acatcggcgg gcatcccgca cttctcgcca
     1861 agttgttcaa gagtcacgtt ccggctctct ctgacactgc ggatcttttc tccaagctgt
     1921 ctgctcattc tctctctcca ctcgaactgg attctgtgcg ggccagtata ccccatccac
     1981 aaggatttga aaagggaaat acgcggacga atcggcagag gacctcagcc cccgcttcat
     2041 gtaccggaga gtcttccatg gatccggaga ttcttccaac cgggaggaag aagcgcctca
     2101 tgtgtgacga tttcaagaga ctcggcgggc ccggctccat cgggaaagcg gagcgcgagg
     2161 tcccgatcgg cacagacagt cagggtaaaa ggaaatcgca attcccgtcc gtcggtcaga
     2221 cggagaacga tctcgtgcag ttccacagcc cggtggtgtg cccgcttgat tgcatacgga
     2281 agagggagag cctcggcaac ggcgctctcg accatggcag gagcggcaaa gcggatgcgg
     2341 atggaccagg gcggcagacc ttcggcacgc cagccccgtc cgtcaagaat catctccggc
     2401 cgtgcccccg ctccatcccg ggaggccaga atttccggcg ctgattttgc gatctcccgc
     2461 actggttctt tttgctgaac acccacggat ccggaatgga cggcatttcg acggaatggt
     2521 tgacagaccc ggtattcccc gcccgagatc atcgcccagg ccgcattgat ctccgccatc
     2581 ctggagacat ccccgccggc atcgggatga tggatccgga cgagggccag ccaggccttt
     2641 ctggctgttt ccctcgaagc atccccggga atccccaaaa tctcccaggg agaaccggga
     2701 ctcacggaac ggcctcatgc aggtccttgc ggatgcgctg cgcgagcacg gtctttccgg
     2761 tgttggccgc ctcatgtccg aggagatagc gcgcccggga gtcatgccag tcctcgtcct
     2821 ctttcaatcc gagcaagcgg gccgcgaccg agatatcgac agagatctgc cccagtgtcc
     2881 gcatcccttc ccgggcccgg tgcagatagc cgatggcgac cgcgggcagc ccctgttcca
     2941 gaaagaattt tccggcggca tagcgggtga ccgggtcctg attccgggcg gcacagagtc
     3001 gccagagcct gccccggact tcacggcaag ccgcatccgg aaagagctca tcgatccgtc
     3061 cgagcagggc cgccgtgagc gggcgccagt ccccgttgtc tcccgctgcc aaaccgacca
     3121 gtgctccctc tatcagggaa gcatctgcat tggtggcccg catcatgcca atttcgataa
     3181 gtgaccgggt ttcgccgctc aggatatggc caaggctgtt gaacaacgtc acggcctcgg
     3241 gaatccgctc ggcccgcgtc aaaagccgca attcccgctc gagatagtag gctgtttcgt
     3301 ccggtggagc acccgggcgc cagagacggg cctgacgaaa atgcatggca gcatcgtggt
     3361 aacatccggc ggcctcgtcg gcccgggctg cagccatgga ccaatagaca ctgcgtctgg
     3421 cacggaacca gccggccagc tccctgacag caaagcgggc cgcctcgggc cggccagccg
     3481 tcaggcaggt ttcgatacca ttgttgagat aatcgaggtt gtatccgctc tcggccacag
     3541 tccggacagc ccttgcgacc agaacagaat tggaggcccc ggttctggca agaccttcca
     3601 gagaaagcag atcaagcgga ttggcctgaa gggatcgtag ataggcggcc tgggcatatt
     3661 ccttgttccc gcagcgctcg aagcagacag ccagtacccg ttcacgatcg ccctggggaa
     3721 acccggaaat cgctgcccat ctccggcagg cctcgatggc tgcggcatgc cgaccggcag
     3781 cgagttccag ttccatgatc ctgttccagg catgggccgt tgccccgaag cgcagactgg
     3841 tccggtacaa ttcgagggct ttggcctctt cccccgagag atcatggctg cgtgccgccg
     3901 caaaccagag attccaggca ttccactgcc aggcaggcga acgggccaag agggaacggt
     3961 actcgctggc tgccgtacgg atcttgttgg tcatgccgca ctgcgtgtag aattcgacaa
     4021 gctgccaacg gaccccgaga tcgaatggcc gggttttgac aagccagaga tgctgttttt
     4081 cgagcgagac aagatcaccg gtgatacggg cactcctgat ccggaaatcc catggctggt
     4141 agagtggtgc cagtatatcc ggaaagatgg ccctttgtgg ttgattcgat gcaaccagtg
     4201 tcgccgaaac ggcaagaatg aggaagaaca cgatcctgat gcaccaggga accggggaga
     4261 atgattttga accgttcgag agtctcatca aaaaaaaata ctcaatccgt gggcgccagt
     4321 cacgaacgcc aacaggtcac ggaaattaaa gagaaggtgg actggcacca aggtcgcaac
     4381 atctcccgag gttcagcatt gagcatccgt tttgtcggat tttctccgga ggacccagag
     4441 cgcgcttgac agggatgaag ggattccata tcttttgaga gtagtgaagt atcgacacag
     4501 ccgggtggtg taagggtaac acagcgggtt ttggtcccgt tattgagggt tcgaatcctt
     4561 ccccggctga tctggtccaa atcccgcgac atactcggcc atcgtatccg ctacctggac
     4621 aaagtcggtc atcccgatca attccaccac ttttcgaaaa tgggctgtca gaccgacaaa
     4681 aagcaccttg cgggactgac tgacagccct ggtcacaagg gaaagcaggg ttgcgactcc
     4741 tgcggaattg acatactttg tccgggagaa ttcgaaagcg atcacttcgg agggatattg
     4801 gagggcgaca tcccatgctt ccttgatcct ggaatcagac tcggcagtga tgtctccctg
     4861 caggagaatc accgggattc cacaaaacat ggtgctttcg agactgaatt cgtatttcat
     4921 gatatcaaca ttgtattctt tcttcttccg gctgtcaatc gggtttcatc gg
//
LOCUS       DAOSHE010000113         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15341_length_4910_cov_4.140062, whole genome shotgun sequence.
ACCESSION   DAOSHE010000113 DAOSHE010000000
VERSION     DAOSHE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_4910_cov_4.140062"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLD82_04400"
     CDS             complement(<1..424)
                     /locus_tag="PLD82_04400"
                     /inference="COORDINATES: protein motif:HMM:NF022676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HOG64660.1"
                     /translation="MHTILDTICDRELSYQQKVHALARDAEATLDVLPIPDAARDLLE
                     RGVICDLFEGHAPWRPRYIIPDYGRFMQQGSDFLRISPPRTLMEATHALLALYHHVPS
                     ITNFPVWIGDIDTLLEPFVEAEPRAGEIIGHFLDHIDRT"
     gene            596..1507
                     /locus_tag="PLD82_04405"
     CDS             596..1507
                     /locus_tag="PLD82_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG64661.1"
                     /translation="MRPSILFSYATLYIVWGSTYWFIKVAVASMPPFWLVALRFLVGG
                     LGFLGVAWLTGRMKGRITRRQILVSGGLGLLLLVGGNGLVTLAETTVDSYLAALVIAS
                     TPLAVALFDRLILKIRIRALCLVGIIIGLAGVGLLLYNGSSIFESLTPGVLLVIAGLV
                     SWAFATSLGHRAGAVPDLVVNSGVQMLTAGLVTGLATLVLGPSPASVLPLIEPEAWLS
                     MAYLGICGSLAFAAYTHLIHNEPAIRVVSYAFVNPLIAMVFGLVISDETATPYLVPGA
                     LMILGGLALMLWGERLFGRKPDPPAGE"
     gene            1647..1967
                     /locus_tag="PLD82_04410"
     CDS             1647..1967
                     /locus_tag="PLD82_04410"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG64662.1"
                     /translation="MSESDSLQIVISRLVPAYNPRQIYLYGSRVWGDPSEWSDLDILV
                     VVEESVLDMDDRMRIGLSALSGSGLDVDILVLTSAEVQARRTYPSTLTYRILHEGKKL
                     YEAA"
     gene            1954..2343
                     /locus_tag="PLD82_04415"
     CDS             1954..2343
                     /locus_tag="PLD82_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOG64663.1"
                     /translation="MKPHEEWIFKAEQDLRSAELLYAAADPVLDVAIYHAHQCAEKSL
                     KSYLSLKQCTIDKTHNLDLLGRRCEEMYSSFIQIREELVFLMPFATAYRYPEGELLPS
                     REKTLKAVCSARLVFDFVSALVRQEDD"
     gene            complement(2357..2674)
                     /locus_tag="PLD82_04420"
     CDS             complement(2357..2674)
                     /locus_tag="PLD82_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64664.1"
                     /translation="MARYRNTVQSGKAPEALRSEIEAYLVREGFKLQEGSDVWKKGIG
                     IMVGPQFVRFAVTSAGLELEAWIKWALLPGVYVGEFDIHGFVGAIPEKLLRDRIKAIE
                     AMA"
     gene            complement(2705..>4910)
                     /locus_tag="PLD82_04425"
     CDS             complement(2705..>4910)
                     /locus_tag="PLD82_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HOG64665.1"
                     /translation="LLVLLSGLLSLPLQAQTPAIRNADRLNLLQGVSALPWTGSPGRA
                     LAIHDGAVQILSDSAGWAGIVAARFGRGRVLVITHDSFYAKAAEGDPGNARFFQNALG
                     WLGRGRILVIGSRPFATLAKKLALPFETAPRPPADLSGYSLIHLDYGWKQSEQLTDAE
                     IIALQHFVQKGGSLMTCVLPWVWKNYSDPGKAGIPVRDSVPNRLLAPMGIGLADWSLG
                     SARPLTHNPEAEHVRVLLAAGLQALEREGSLPVDWLNAFMERTRSVFDMVPHAGAILT
                     ENEINRIRTVFSTLGPRTVPSEKRPITPADSRAVIAITLMDALARNPPPGFVVETLPH
                     AEIHPGAPAPGSTPVTRTFKIEPDGKGRWQSTGLWARAGKPVRVRILSGNPREVSLVI
                     GIHQDNLFKFNPSITQWKRWPDLVLRASLATGSASITSPHGGLVSIDVPDGSQDELEV
                     RIEGAIPAARFTKGVSPTSWRRNLARTQAPWGEIEGQNIIISAPLAALKNLTDPEGII
                     AWWDRVVLLYPALSGKPLHPYKQRFVPDVQISMGYMHSGYPVMTWMDVAGWVLGVPGD
                     KPRNHVLTNGAWGHFHELGHNYQMGEYTWEGAGEVTVNIFTLHTMEKMGIVPAVSNPR
                     ILKARRDLATKYNGRLPFERWSSEPFIALLFYVDLVKGFGWTTMGKVLAEYAGLAKDD
                     RPQTNAEKRDQWLIRFSKACGRNIAPHMHRWNIPFSAGAEAQLRSLPVWNGP"
BASE COUNT          943 a         1321 c         1543 g         1103 t
ORIGIN      
        1 gcgtgcggtc gatgtgatcg agaaagtgtc cgatgatttc cccggcccgg ggttcggctt
       61 cgacaaaggg ttcgagcagg gtgtcaatgt caccgatcca gaccgggaag ttcgtgatgg
      121 atggtacatg gtggtagagc gccaaaaggg cgtgggtggc ctccatcagg gtgcggggcg
      181 ggctgatgcg caagaaatcg gaaccctgct gcatgaagcg tccgtaatcg ggaatgatgt
      241 agcgcggacg ccagggagcg tgaccctcga agagatcgca gatcacgccc cgctccagaa
      301 gatcgcgggc cgcgtccggg atgggcagga catcgagggt cgcctcggca tcccgggcca
      361 gggcgtgtac cttttgctgg tatgacagtt ctcggtcgca gatcgtatca aggatggtgt
      421 gcatggtgtc ctcccggcct ctcatcccag tatagcagaa tttgaatgtt tcggcaagcg
      481 cgcttgcggt cggtagaaat tacggaattg tgatgggatt ccggaataag catcatggat
      541 ttgtcatgcg aatccgggat tgccttcaga cttgtgtgat gactaccctt accccatgcg
      601 tccatcaatc ctcttttcgt atgcgactct ctacattgtc tggggttcaa cctactggtt
      661 catcaaggtg gccgtcgcga gtatgccgcc gttttggctg gtcgctctcc ggttcctggt
      721 cggggggctc ggttttttgg gggtggcctg gctgacaggg cgcatgaagg gacggatcac
      781 ccggcgccag atccttgtgt ctggtggtct gggcctcctc ttgctggtgg gcgggaacgg
      841 tctcgtgacc ttggcagaga cgaccgtcga cagctatctg gctgcccttg tgatcgcatc
      901 gactccgctg gccgtagccc tctttgaccg cctgatactg aaaatacgaa tccgtgcgct
      961 ctgtctcgtc gggatcatca tcgggctggc cggagtgggc ttgttgctat acaacggatc
     1021 ttcaatattt gaaagtctca cacccggagt gttgttggtg atcgccggtc tggtgagctg
     1081 ggcctttgcc accagcctcg gacaccgggc cggggcagtg cccgatctcg ttgtcaactc
     1141 aggggtacag atgctgacgg ccgggcttgt gaccggtctg gcgactcttg tcctcggacc
     1201 atcgcctgcc agcgtccttc ccctgatcga acccgaggcc tggctctcga tggcctatct
     1261 gggcatctgt ggctcgctgg cctttgccgc ctatacccat ctgatccata acgaaccggc
     1321 catccgggtg gtgagctacg cctttgtcaa tcccctgatc gccatggttt tcggactggt
     1381 catcagcgac gagacggcaa caccctatct cgtgccgggg gcactgatga ttctgggagg
     1441 actggccctt atgctctggg gggagcgcct tttcggccgc aagcccgacc cgcctgccgg
     1501 ggaatgacga aattaccttt acagtgagga aatcattacg gaacaagggc gaatttttct
     1561 ctcgtgttgc atcgttcttg tttcgacatc cctcttcgtg tatagttatg aaagaccggt
     1621 tgacacattt tttatggagg cgggaaatgt cagaatccga ctcactgcaa atcgtgatat
     1681 cccgtcttgt gcctgcctac aatccgagac agatatacct ctacggctcc cgagtttggg
     1741 gtgatccatc cgagtggagc gatcttgaca tcctcgtggt cgttgaagaa tcagtactgg
     1801 atatggatga ccggatgcgg atcgggttga gtgccctgag tgggtcgggg ttggatgtcg
     1861 atattcttgt tcttacgtct gccgaggttc aggctcgtcg tacatatcca tcaaccctga
     1921 cgtacaggat tcttcatgaa ggtaaaaagt tgtatgaagc cgcatgaaga atggattttc
     1981 aaagcggaac aggatcttag atcggcagag ttactgtatg ctgctgctga ccctgtgctg
     2041 gacgttgcca tttatcatgc ccatcagtgc gcggaaaaat cgctcaaatc gtatctctcg
     2101 ctgaagcaat gtacgataga caagacgcat aatcttgatc ttcttggcag aagatgcgaa
     2161 gaaatgtatt cctcgttcat ccagatccgg gaagaacttg tatttctcat gccatttgct
     2221 acagcgtacc ggtatccgga aggtgaactc cttccttcca gggaaaaaac cctcaaagcc
     2281 gtatgcagtg cacgattggt attcgatttt gtcagtgctc ttgtcaggca ggaggatgac
     2341 tgacacggtt tgtccgtcag gccatggcct cgatggcctt gatccggtcc cgcaggagct
     2401 tttcggggat ggccccgaca aagccgtgga tgtcaaactc gcccacatag acgccgggca
     2461 ggagcgccca cttgatccag gcttcgagtt cgagccccgc ggatgtgaca gcaaagcgga
     2521 caaactgcgg accgaccatg atcccgatgc cctttttcca gacatcggac ccctcctgga
     2581 gtttgaaccc ctcgcgcacg aggtaggctt cgatttcgga ccgcaaggcc tccggtgcct
     2641 tgcctgattg tacggtattg cgatagcgtg ccattatggt ctcctcttgt gatgggtcag
     2701 tatatcacgg accattccaa actggaagag agcgtagctg tgcctcggcc ccggcactga
     2761 aggggatgtt ccagcggtgc atgtggggcg cgatgttcct tccgcaggct tttgaaaaac
     2821 ggatcagcca ttggtcgcgt ttttcggcat tggtttgtgg gcggtcatcc ttggccagtc
     2881 cggcatattc cgccagaacc ttgcccatcg tggtccagcc gaagcctttg accagatcga
     2941 cataaaagag aagagcgata aagggctcgc tgctccagcg ctcaaacggg agacgcccat
     3001 tgtattttgt ggccagatca cgcctcgcct tgaggatccg cgggttgctg acagccggga
     3061 cgatccccat tttttccatg gtgtgcaggg tgaagatgtt gaccgtgacc tcgccggcgc
     3121 cttcccaagt gtattcaccc atctggtaat tgtggcccag ttcgtgaaaa tggccccagg
     3181 ccccattggt caggacgtgg ttgcggggct tgtcccccgg aacaccgagg acccagccgg
     3241 cgacgtccat ccaggtcatg accggatagc ccgagtgcat ataccccatg gagatctgga
     3301 catccggtac aaagcgctgt ttgtacgggt ggagcggttt gccggagagt gcgggataga
     3361 gcaggacaac ccggtcccac caagcgatga tgccttccgg atcggtcagg tttttgaggg
     3421 cggcaagtgg tgcgctgata atgatgttct gtccttcgat ttcaccccaa ggggcctgtg
     3481 tccgggcgag gtttcgtcgc catgaagtgg gtgaaacccc tttggtaaac cgggcggctg
     3541 gaatggcacc ttctattcga acctcaagct cgtcctgcga gccgtcaggg acatcgatgg
     3601 agaccagccc tccgtgtggt gatgtgatgg acgccgaacc tgtggccaga ctggcccgga
     3661 ggaccagatc cggccagcgc ttccattggg tgatcgaggg attgaatttg aagagattgt
     3721 cctggtggat accgatgacg agcgaaacct cgcgtggatt gcccgagagg atccggacgc
     3781 ggaccggttt tccggcccgt gcccagagtc cggtgctctg ccagcgtccc tttccgtctg
     3841 gttcgatctt gaaggtccgg gtgaccggtg tgcttccggg tgcgggtgcc ccaggatgga
     3901 tctcggcatg ggggagtgtc tcgacgacaa agcccggtgg cgggttacgg gccagggcgt
     3961 ccataagggt gatggcaatc acggctctcg agtctgcggg cgtgatcggt cttttttcac
     4021 tgggaacagt cctcggtccg agggtcgaaa agacggtccg gatcctgttg atttcgtttt
     4081 cggtgaggat ggcccccgca tgtggaacca tgtcaaagac actgcgggtg cgttccataa
     4141 aggcgttgag ccagtcgacg ggcaggctcc cttcgcgttc cagggcctgc agaccggcgg
     4201 ccaggaggac acggacgtgt tcggcctcgg gattgtgtgt cagcgggcgg gcgctcccca
     4261 gagaccagtc ggccagaccg atgcccatgg gcgcgagcag gcggttggga acggaatcac
     4321 ggacaggaat cccggctttg ccgggatcgc tgtagttctt ccagacccag ggcaggacgc
     4381 aggtcatgag cgagccgccc ttctggacaa agtgctgcag ggcgatgatc tcggcatccg
     4441 tcaattgttc ggactgtttc cagccataat caagatggat cagggaatag cccgagaggt
     4501 cggccggggg acggggggca gtctcgaagg ggagagcgag tttttttgcc agcgtggcaa
     4561 agggccgtga gccgatgacg aggatccgtc cccgaccgag ccatccaagt gcgttctgga
     4621 aaaagcgggc gttgccgggg tcgccttcag ccgcctttgc atagaagctg tcatgcgtga
     4681 tgaccaggac ccgcccgcgc ccgaagcggg ctgcgacgat tccggcccag cctgcgctgt
     4741 cagacaggat ctggaccgcc ccgtcatgga tggccagggc ccggccgggg gagccggtcc
     4801 aagggagggc gctcacgcct tgaagaaggt tcaggcggtc ggcattgcgg atggcagggg
     4861 tttgtgcctg aagcggcagg gagaggagtc ccgacaagag gacaagaagg
//
LOCUS       DAOSHE010000114         4897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15389_length_4897_cov_4.057621, whole genome shotgun sequence.
ACCESSION   DAOSHE010000114 DAOSHE010000000
VERSION     DAOSHE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15389_length_4897_cov_4.057621"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="PLD82_04430"
     CDS             complement(<1..1581)
                     /locus_tag="PLD82_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64666.1"
                     /translation="MTQPTRLLLILVLTLPLSASDTSRPFAEAEQAMASGQTAQALLL
                     YQGLATHPDQRIAGYAAFFSGQTLLRLNRAEEAIPWLARAEETARSLGQPSLLLYARF
                     HLAIADGMLGKTGQALARLDQIPASGHAPEDTPLILRSRIESLRLSALLLQTSPGQIA
                     RQTLLHKRDALLDLVQRSPQTVKGQSVELETALEAVLACGDPAAADGLITLLDALPPE
                     ERNSRSPLKARALALAGKTMEALRLLPADPGAALPPVLARVGILAASGQRDLAAKEAS
                     AAMAGRSVEERARLGGVAGHLWLDLGRFAEAAPLIELAMRSSDPKVRTQALADGASLA
                     LASGDSRTAIRRIRESGLKSEAAIRLEASALLASTSPSQAEALLRNLTEKTNTPALAL
                     LRAQILTMGATNDETHRLAQVQANTALRLARASDDTSRIAWASILLARLSSGTNAAAI
                     LEQILADDGAGSDLRARATAFHDLGLLALREDRPSEALTLLRQAARLFETWRAPWGRQ
                     DRLHSSANWAGTRRHMALA"
     gene            1872..3611
                     /locus_tag="PLD82_04435"
     CDS             1872..3611
                     /locus_tag="PLD82_04435"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG64667.1"
                     /translation="MRISNLVLPSLKEIPAEAVIASHQLMLRAGLIRKMTSGIYSYLP
                     LGLKALRKVERIIREEMDRAGAQEFMLPLLLPREIWEETGRWAVMGDLMMKVHDRAGK
                     DSVLAPTAEEGFTSILRDELKSWRQLPCTVYQIGKKFRDEIRPRFGVMRGREFIMKDA
                     YSFDRDQKDLDASYEAMRTAYYRIFKRLGLEVTGVAADSGAMGGSGSEEFMVPSTVGE
                     NEIVVCRACNRAANVETARCRQDLPAQSGAGSLSLEKVSTPAVRTIEELCTFFSCDAA
                     QFLKSLVYTCEKDGKKAFALAVIRGDLDVNEVKLANALGVPEVALAGEEEVRTVTGAP
                     VGFAGPVGLSGVPVVADLTLKAVVDGITGANERDCHYKGVNHGRDWTATLWADLRTVR
                     EGDPCEHCGQPLAVFRGIEVGHIFKLGDKYTKSMGVTYLDENGQNKTPIMGCYGIGVD
                     RSLASVIEQHHDQDGIIWPASVAPYDVVIVPINWAHEETQAAALKLTAELEGLGLEVL
                     LDDREARAGFKFKDADLFGFPLRITIGEKTLATGEVEWKRRADGEGGRVALAAAAEAV
                     AAMHRELLAACQV"
     gene            3752..4090
                     /gene="yajC"
                     /locus_tag="PLD82_04440"
     CDS             3752..4090
                     /gene="yajC"
                     /locus_tag="PLD82_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG64668.1"
                     /translation="MNALFMLSQAGCTPQGGAQGGQGGSSIMFLVMMVAMIGIMYFVV
                     YRPQAKARKKLEAAINEMKKGDRVLTAGGIYGTVVGTSETVVVLEIDKDKGVKIEVNK
                     AMVSQVLPRE"
     gene            4202..4516
                     /locus_tag="PLD82_04445"
     CDS             4202..4516
                     /locus_tag="PLD82_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64669.1"
                     /translation="MLTVGAFAFPRVLSAADDLNKEFVENFFKLRPDDADVFLKIQKS
                     GAKSGTEAALENPVSNTNAGSGSDENDPSWIGKFLQLSILGLLGLVIYLMSRRSRSRA
                     RY"
     gene            4568..>4897
                     /locus_tag="PLD82_04450"
     CDS             4568..>4897
                     /locus_tag="PLD82_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64670.1"
                     /translation="MKFKLRTVVVILTLLLCWWGLKDTIEWYFVMPESEKDLIGLSLE
                     EVDALSTNQKAKVLALKEKRKRVLNLGLDLQGGLYLVLSVDEKSLTEDLVARYTVDLK
                     TSTNGTFR"
BASE COUNT          900 a         1368 c         1650 g          979 t
ORIGIN      
        1 cgccagagcc atgtgccggc gggtcccggc ccagttcgcg gaagagtgga ggcgatcctg
       61 tctcccccag ggagcccgcc aggtctcgaa gagcctcgcg gcctgcctga ggagggtcaa
      121 tgcctcggac gggcggtcct cgcgcagggc gaggagcccc agatcgtgga aggccgtggc
      181 ccgagcacgg agatcgcttc cggctccgtc gtccgcgaga atttgttcga ggatggcggc
      241 ggcattggtt ccgctcgaca agcgcgcgag gaggatggag gcccaggcga tacggctcgt
      301 gtcatccgat gcgcgggcca ggcggagcgc ggtgttcgcc tgaacctgag ccaggcggtg
      361 tgtttcgtcg ttggtcgcac ccatggtcag gatctgggcc cggaggagtg ccagcgccgg
      421 ggtatttgtt ttttcggtga gattccggag gagggcctct gcctgggagg gggatgtgga
      481 cgccaggagt gcgctggcct cgaggcggat ggcggcctcg ctcttcagtc cgctctcccg
      541 gatccggcgg attgccgttc ggctatcccc ggaggccagg gccagggatg ccccgtcagc
      601 cagggcctgc gtccggacct tcgggtcgct ggagcgcatg gcaagttcga tcaggggggc
      661 ggcctcggca aaacgtccga ggtcgagcca gagatggccg gccacgccac ccagtctggc
      721 ccgctcttcc acggagcggc ccgccatggc ggcactggct tctttggcgg ccaaatcgcg
      781 ctgtcctgaa gccgccagga ttccgactct ggccagaacg gggggcaggg ccgcccccgg
      841 atccgccgga aggagtcgca gcgcctccat ggtttttccg gccagggcca gggcgcgtgc
      901 cttgaggggg ctccggctgt tccgctcctc aggcgggagg gcgtcgagca gggtgatcag
      961 tccgtcggct gctgccgggt ctccgcacgc gaggacggct tcgagggccg tctccagttc
     1021 gacgctctgg cctttgaccg tttggggaga gcgttgcacg agatcaagga gtgcatcccg
     1081 cttgtgcaac agggtctgcc gtgcgatctg tccgggggag gtttgcagca ggagggcgga
     1141 gaggcgcagg ctctcgatcc tggagcggag aatgaggggg gtgtcctccg gagcatgtcc
     1201 gctggccggg atctggtcaa ggcgggcgag ggcctggccg gttttgccga gcatcccgtc
     1261 ggcaatggcc agatggaagc gggcgtagag gaggagtgag ggctgaccaa ggcttctggc
     1321 cgtctcctcg gcccgtgcca gccaggggat ggcctcttcg gcgcggttga gccggagaag
     1381 ggtctgtcct gaaaagaagg cggcgtatcc tgcgatccgc tgatcgggat gggtggccag
     1441 gccctggtag aggaggagag cctgtgcggt ctgtccggag gccatggcct gttccgcctc
     1501 ggcaaaagga cggcttgtgt cactcgcgga cagggggagg gtgaggacga ggatcaagag
     1561 gaggcgtgtg ggttgcgtca tctggtttct ccggacatcg gggttccacc ggggggcggt
     1621 cgctcgatgg ccacaggttt tttgtcactc tggaagacat gacaccgatt gaaggcgggg
     1681 ttcattcttc ggtattgtcc agctttacgt gccggtgatg gttccgcaga agcggcagag
     1741 atacaaaaat cgctcgggtc cagcccgtcg aggcggggga atttgacaaa tcttccctgc
     1801 ccgacctata ttttgcatct gtccctccat aacaatacat ggtgagcgcc cgggcgcaaa
     1861 ggagtcatgc tgtgcgaatt tcgaatcttg tcttgccatc cctcaaggaa atcccggccg
     1921 aggctgtcat cgccagccat cagctcatgc tgcgggccgg cctgatccgg aaaatgacat
     1981 cgggaatcta ttcctacctt ccattgggtc tcaaggccct gcgcaaggtc gaacgcatca
     2041 tccgtgagga gatggaccgg gccggcgccc aggagttcat gctccccctg ctcctgccgc
     2101 gggagatctg ggaggagacc gggcgttggg cggtgatggg tgacctgatg atgaaggtcc
     2161 atgaccgtgc gggaaaggat tccgtgctgg ctcccaccgc agaggagggc tttacctcga
     2221 ttttgcgcga tgaactcaag tcctggcgcc agcttccctg taccgtctac cagatcggaa
     2281 agaaattccg tgacgagatc cgtccgcgtt ttggcgtgat gcgcggacgc gagttcatca
     2341 tgaaggatgc ctattccttt gaccgtgacc aaaaggacct ggatgcttcc tacgaggcga
     2401 tgcgcacggc ctattaccgg atcttcaagc gcctcggcct cgaggtgacc ggggtggccg
     2461 cggattcagg tgcgatgggc gggagcggga gcgaggagtt catggtcccg tcgaccgtcg
     2521 gtgagaacga gatcgtggtc tgtcgtgcct gcaaccgggc ggccaatgtc gagacggccc
     2581 gctgccgcca ggatcttccc gcccaatccg gcgcgggaag tctctccctt gaaaaagtct
     2641 ccacgcctgc ggttcgcacc attgaagagc tctgcacctt cttctcctgt gatgccgccc
     2701 agttcctcaa gtccctggtc tatacctgcg aaaaagacgg aaaaaaagcc ttcgccctgg
     2761 ccgtgatccg gggtgacctg gatgtgaacg aggtcaagct ggccaatgcc ctgggcgtcc
     2821 ccgaggtggc actggccgga gaggaagagg tgcgtaccgt gaccggtgct ccggtcggtt
     2881 ttgccggacc ggtcgggttg tccggagttc ccgtggtggc cgacctgacc ctcaaggctg
     2941 ttgtggacgg gatcaccggt gcgaatgagc gggactgcca ctacaagggc gtcaatcatg
     3001 gcagggactg gacggcgaca ctctgggccg acctgcgaac cgtgcgcgaa ggggatccct
     3061 gcgagcattg tggtcagcct ctcgcggtct tccgcggtat cgaggtcggg cacatcttca
     3121 aactgggtga caaatatacc aagtcgatgg gtgtgaccta tcttgacgag aacggacaaa
     3181 acaagacacc catcatggga tgttacggga tcggggtaga ccgcagtctg gcctccgtga
     3241 tcgagcagca tcatgaccag gacgggatca tctggccggc ctcggtcgcg ccctatgatg
     3301 tcgtgatcgt cccgatcaac tgggctcacg aggagacgca ggccgctgca ctcaagctga
     3361 cagccgaact ggaaggactc gggctcgagg tgcttcttga tgaccgggag gcccgggctg
     3421 gattcaagtt caaggatgcg gatctcttcg ggtttccgct tcgcatcacg atcggcgaaa
     3481 agacgctggc cacgggtgag gtcgagtgga agagacgggc tgacggagag ggtggccggg
     3541 tggcactcgc ggccgctgcc gaagcggttg cggccatgca ccgtgaattg ctggcggcgt
     3601 gtcaggtctg agacggtttt tggggaacaa actcgggcca gtcagctggc ggtgggcccg
     3661 gatcgtgctc cggccttgct tttgggcgag tatttccata cattaaggcg gtatcagcta
     3721 ctttgtgcac ttccatcaag gagaatcaat aatgaacgca ttgttcatgc tttcccaggc
     3781 cggctgcacc ccccagggcg gcgcgcaggg cggtcagggc ggcagcagca tcatgttttt
     3841 ggtgatgatg gttgccatga tcggcatcat gtattttgtc gtctaccggc cgcaagccaa
     3901 ggcccgtaaa aaacttgaag ctgccatcaa cgagatgaaa aagggtgacc gcgtcctgac
     3961 ggccggcggt atctacggca cagttgtcgg gacgagcgag actgttgttg ttctcgaaat
     4021 cgacaaggac aaaggtgtca agatcgaggt caacaaggcc atggtctccc aggttttgcc
     4081 aagagaataa gcgcaggcag tgggttccgc gatccgtcgg gtctgcggga tccgccctga
     4141 catgactgat agaagggaat acggggtgag gaagtctctg acaacgtgga tttcactggc
     4201 agtgctgact gtcggtgctt ttgcgtttcc ccgggtcctg tctgcggccg atgatctgaa
     4261 taaagagttt gtcgaaaact ttttcaaact ccggcccgat gacgcggatg tctttctcaa
     4321 gatccagaag agtggagcca aatccgggac ggaagcggct ctggaaaacc cggtatccaa
     4381 tacgaatgcc ggctccggat ccgacgaaaa cgatccgtcc tggatcggga agttcctgca
     4441 actctccatt ctcgggttgc tcggtctggt catctacctc atgtcacgca ggtcgcgttc
     4501 gcgcgcccgc tattgaagtc cggcgcggca gggttgctcc ccgccgcgta ccacgtttgg
     4561 aggctacatg aaattcaagc tgcgaacggt agtcgtgatc ctcaccctgc ttctctgctg
     4621 gtggggattg aaagatacca ttgaatggta ttttgtcatg cccgaatcgg aaaaggatct
     4681 gatcgggctc tccctcgaag aggtggatgc cctctctacc aaccagaaag ccaaggtcct
     4741 ggctctcaag gagaaacgga aacgggttct caacctgggg ttggatcttc agggcggtct
     4801 ctatctcgtt ctctctgttg atgagaagtc cctgacagag gatctggttg cccgctacac
     4861 cgtggatctc aagacatcca ccaacggaac tttccgg
//
LOCUS       DAOSHE010000115         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15499_length_4870_cov_4.124818, whole genome shotgun sequence.
ACCESSION   DAOSHE010000115 DAOSHE010000000
VERSION     DAOSHE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15499_length_4870_cov_4.124818"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..2498
                     /locus_tag="PLD82_04455"
     CDS             360..2498
                     /locus_tag="PLD82_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64671.1"
                     /translation="MQTAMKICVCGMVCAGLVSACGDDPQSAGFVQSGHTLVRNGYAL
                     TDYTVPAGTSELVVTNLRQQERIVIIPLDSEYATGPAKLRQVGTLRVEYAGPAPRELA
                     PRHRLEAVPHNRISARDKAAAFSGTARLDAFQRLPARHPESKKQPASRATEFSLGQTH
                     DFFVIYDFNDIAKGGVAASSRLLYKGTHCYVWLTAGIATHANAGPVATNAGQAFDATI
                     YQKVTGLFGSEWGGERDDGSGGIDGDKRVHILFQDVTKNPPGGYCSGRDQYYNTQFSK
                     PEDRYSNEKEMFYICQPATYMDTIPNRNDVLAHEFQHLVHHNQVRRKQDASPLSYINE
                     GFSQLAETWCGYGPMVPRFADVQNRPRTSWLVWYMEGRDRVDSYYAAYVLANYLVRRF
                     GLEALTTLRDAPPSSQANLDAALGRLGYDGDWPRLVLDMLTALWLDRDEFSFPAVGLH
                     PGDSGIPMVGTRPYWTQEKNGFSNKTNLLNLQGARTRFLTSFPATIPLELYQYGSDMF
                     ELVSGVGGQITLRFENTSVPLRVRVLRFGGEEAGVSVPFAIDGYGADWNGRTGLVSAP
                     DPAGDAIMVNGKPSQFTCDPGADMTNVLAFCSNDVLYMRIQTVGPMHPTDKTNRLMLK
                     LRLSGSADQFNVYCYDSRFFAYLIGATNEYPLTTYGVRYARRSVIELALPIGIFGITP
                     DASIRVQPLMFNIILTSNVFDWGPAVTVTR"
     gene            complement(2560..3393)
                     /locus_tag="PLD82_04460"
     CDS             complement(2560..3393)
                     /locus_tag="PLD82_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64672.1"
                     /translation="MKRSSRFVRIKFFLLLLAAIPIVLGTGLAIHSSLLSHGVSALQS
                     SLVTTDSAKSQSGRSGPETHPAGRPLTELAAHLERYSTLGLIAGIAGLLLTIILIVTL
                     HRGRLGIYRSFFRKALHTASERTVLDLDALRFPDEDDLGQLGLALNGIVSRLREYDAL
                     RRDELLLADSTVRTVISTHEQALAVFDDQLTLVFFSGIFSDLLGGRSKAGLKTSDLFT
                     DATTLEQLVQSLHKDGKTELPQRPAADTIIPLLCVTLTVQKSSARRLLVQFGKASGDY
                     V"
     gene            complement(3390..4103)
                     /locus_tag="PLD82_04465"
     CDS             complement(3390..4103)
                     /locus_tag="PLD82_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOG64673.1"
                     /translation="MNRSLIIGLGTFGMTIAEELSAKGAEIVAVEVNESVMHQVSGKI
                     PDVRVIDPSDPAWLGAIDPDSVDHAVVCIGNLEQSLLMTLQLVEHGFANIHARASTEA
                     HRRILLSIGIRNVIFPELDAAKALADRITAPSIRSIVRLAGDKIMADFAIPMVFAGQS
                     LVELDLRRRYNVTVIALRRFVPAIDSLTRENIMREQFLDAPKPDDPLEEGDVLVLVGT
                     EENIHSLIEAWVDDDGDTA"
     gene            complement(4108..>4870)
                     /locus_tag="PLD82_04470"
     CDS             complement(4108..>4870)
                     /locus_tag="PLD82_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG64674.1"
                     /translation="MLLIILGGIGFVVIGETRVWLADRLFLKKKTWLSLHSRLALATT
                     FFLILFGAVFFFFMEQGATLKESGTGESVLASLFQSVTTRTAGFNSLDFAAMTPATLF
                     LCIALMFIGASPGSTGGGIKTTTFAVIWFAIGSIIRDRSVVVAGRRAIPREIVNKALA
                     ITGCFLALIFLSTLLILVIQPGIEFLKVLFEVVSASATVGLSTGITAGLEPLAKLVLI
                     GTMFLGRIGPLTLIIAFGSRSPTDLRYPRERIMTG"
BASE COUNT         1037 a         1299 c         1518 g         1016 t
ORIGIN      
        1 cttggctctt ttcttgacat aaagagcgcg taaggggcga tcaggcgaac cttgtcaaac
       61 tggcgccgct cctctgctcg aatcagttta taaatttcag ggtcccgttg tgaaagatga
      121 tccatgggca ggtctccttt tttttgggaa cagtgtacaa gttcccggtc gtgttcgtat
      181 ttcccgaaaa gggggcgctc aggtgacgta tccattggga tgacggcaga gggcggacag
      241 ccttcggatg cctgaaaaat agtcaaattg cccggttttt tgtccgccat tttactgaat
      301 ggaagacggg gtgtggtatc ctgagatcag gatacttggg atcagccgtg gagggtgtga
      361 tgcaaacagc catgaagata tgtgtatgcg gaatggtgtg tgccgggctg gtgtccgcct
      421 gcggagatga tccacagtct gctgggtttg tccagagcgg gcacacgctg gtgcgaaacg
      481 ggtatgcact gacggactat acggtccctg ccgggacgag cgaattggtt gtgaccaacc
      541 tccgccagca ggaacggatc gtgatcatcc cgctggacag cgaatatgcg acagggccgg
      601 ccaagctgcg acaggtcggt accctgcggg tggagtacgc aggccctgcc ccgcgcgagc
      661 tcgcgccgcg gcacaggctg gaggctgttc cccataaccg catttctgcc agggacaagg
      721 ctgcagcgtt ttcgggtacg gcccggcttg atgcttttca acgcctgcct gcccggcacc
      781 ccgagtcgaa gaaacagcct gcaagccggg cgaccgaatt cagcctgggt caaacccatg
      841 atttttttgt gatttatgac ttcaatgata ttgcaaaagg gggagtggcc gcctcgtccc
      901 ggctgctgta caaagggacg cattgttacg tctggctgac ggcggggatc gccacccatg
      961 ccaatgccgg gcccgttgcc accaatgccg gccaggcctt tgatgccacg atctaccaga
     1021 aggtgaccgg cctttttggt tccgaatggg gcggggaaag agatgacgga agtggcggga
     1081 tcgacggtga caagcgggtg cacatcctct ttcaggatgt gaccaaaaac cctccgggcg
     1141 gatactgcag cgggcgtgat cagtattaca atacccagtt ttccaagcca gaggaccgct
     1201 acagtaacga aaaagaaatg ttctacattt gccagcctgc gacctatatg gacacgatcc
     1261 ccaaccggaa tgacgtattg gcgcacgagt ttcagcatct tgtccaccac aaccaggtgc
     1321 ggcgcaagca ggacgccagt cccctgtcgt acatcaatga gggcttctcc cagcttgccg
     1381 agacttggtg cgggtatggt ccgatggtgc cccgttttgc ggatgtgcaa aaccggccgc
     1441 ggacatcctg gctggtctgg tacatggagg ggcgcgatcg ggtggactcg tactacgcgg
     1501 cctatgtgct cgcaaactat ctggtgcgcc gctttgggct cgaggcgctc acgacgctca
     1561 gggatgcgcc cccttcaagc caggccaatc tggatgcggc tcttggccgg ctcggatacg
     1621 atggcgattg gccgcgtctc gtgctggaca tgttgaccgc cctctggctg gatcgtgacg
     1681 aattttcttt tccggcggtc gggctccatc ccggggacag cgggatcccg atggtcggga
     1741 ctcgtccgta ttggacacag gagaaaaacg ggttttccaa caagaccaat cttctcaatc
     1801 tccagggagc gcgcacccgg tttttgacat ccttccccgc gacgattccg cttgagttgt
     1861 accagtacgg atcggacatg ttcgagctgg tcagcggggt cggcggtcag atcaccctgc
     1921 gctttgaaaa cacgagtgtt ccgttacgtg tgcgggtcct gcggtttgga ggggaggagg
     1981 cgggagtctc cgtcccgttt gcgattgatg gatatggagc ggactggaac ggtcggacgg
     2041 gactggtgtc tgcgccggac cctgcgggag atgcgatcat ggtcaatggc aagccctcgc
     2101 aattcacgtg cgatccgggt gccgacatga ccaatgtcct ggctttttgc agcaatgatg
     2161 tgctctacat gcggatccag acggtcgggc ccatgcaccc gactgacaag accaacaggc
     2221 tcatgctcaa gctcaggcta agcgggagcg cagaccagtt caacgtctac tgctatgaca
     2281 gccggttttt tgcctatctg attggagcta caaatgagta tcccctgacg acgtacggtg
     2341 tgcgctacgc ccggcgttcg gttatcgagt tggctttacc catcgggatc ttcggtatca
     2401 cgcctgacgc ctccatccgg gtgcagccct tgatgttcaa tattatcttg acttcgaatg
     2461 tctttgactg ggggccggct gtgacagtga cgcgctgaga gattccggac tccaagtgcg
     2521 caacgggatc cggaatccag gaggactcgt gaaagagggt cacacgtagt cgccgctggc
     2581 ctttccgaat tggacgagga gacgcctggc agaagatttt tgcactgtca gggtgacgca
     2641 gaggagaggt atgatcgtat ccgctgccgg ccgttgcggc aactcggtct tcccgtcctt
     2701 gtgcaagctc tggacaagtt gttcaagagt tgtcgcatcg gtaaagagat cgctggtttt
     2761 gagtccggcc ttggaccggc ctcccagcaa gtccgaaaag atgccggaaa aaaacaccag
     2821 ggtcaactgg tcatcaaaga ccgccagtgc ctgctcgtgc gtactgatca ccgtccgcac
     2881 ggtcgaatca gccagcagca attcatcccg tcgcaacgcg tcatattcgc gcagacggga
     2941 gacgattccg ttcaaggcca ggccgagttg cccaagatca tcttcgtcag ggaagcgcag
     3001 ggcatcaagg tcaaggacgg ttcgttcgga ggcggtatgc aatgctttgc gaaaaaaact
     3061 gcggtagatt cccagccgcc ctctgtggag ggtgacgatc aggatgatgg tcaggaggag
     3121 tccggcaatc ccggcgatca ggccgagggt gctgtagcgt tccagatgtg cggcgagttc
     3181 ggtcaatggc cttccggcag gatgtgtttc cggaccggat cgtccggatt ggcttttggc
     3241 cgaatccgtt gtgacaaggg acgattggag tgcgctcaca ccgtgcgaga ggagtgagga
     3301 atggatggcc aggccggtcc ccaggacgat cgggatggca gccagcaaca gaaggaagaa
     3361 tttgattcgt acaaagcggg aggaacgttt catgctgtat ctccgtcatc gtctacccag
     3421 gcctcgatca ggctgtggat gttttcttcg gttccgacaa gaaccaggac atccccttcc
     3481 tcgagagggt catccggttt gggtgcgtcg agaaactgct cgcgcatgat gttttcccgg
     3541 gtcaggctgt cgatggcagg gacaaagcgc cgcagggcga tcacggtcac gttgtagcgg
     3601 cgccgaagat caagctcgac cagggattga ccggcaaaga ccatggggat ggcaaaatcc
     3661 gccatgattt tgtcaccggc gaggcggacg atggatcgga tgctgggggc ggtgatccgg
     3721 tcggccagtg ccttggcggc gtccagctcc ggaaagatca cattccggat cccgatcgag
     3781 agaaggattc tccggtgggc ctcggtcgat gcccgggcat ggatgttggc aaagccatgt
     3841 tcgaccaatt ggagggtcat caggagggat tgttccagat tgccgatgca gacgacagcg
     3901 tgatcgaccg aatccgggtc gatggcaccg agccaggcgg gatcggacgg gtcaatcacg
     3961 cggacgtcgg ggatttttcc ggagacctgg tgcatcacgg actcgttgac ttcgacggcg
     4021 acgatctcgg cgcctttggc cgagagttcc tcggcaatcg tcatgccaaa agttccaaga
     4081 ccgataatca ggcttctgtt cattctctca tcctgtcatg attcgttcgc ggggatagcg
     4141 cagatcggtc ggggaccggc tgccaaaggc aataataagc gtcaagggtc cgatccggcc
     4201 gaggaacatg gttccgatca gaaccagctt ggcgagcggt tccaggccgg ccgtaattcc
     4261 ggtcgaaagc ccgactgtcg ccgatgccga tacgacctca aagagcactt tgagaaactc
     4321 gatcccgggc tggatgacca ggatcagaag ggttgaaaga aagatcaagg ccagaaagca
     4381 tccggtgatg gccagtgcct tgttgacgat ctcgcgggga attgcccgcc gaccggcaac
     4441 gacaacgctc cggtcccgga tgatgcttcc gatggcgaac cagatcacgg caaaggtcgt
     4501 tgtcttgatt ccgcctccgg ttgaacccgg tgaagcaccg atgaacatca gggcgataca
     4561 caaaaacagg gtggccggag tcatggccgc aaaatcaagg ctgttgaaac cggctgttct
     4621 tgtggtgaca ctctgaaaca gggaagcgag gaccgactcg cccgtcccgc tctccttgag
     4681 ggtggccccc tgttccatga aaaagaaaaa aacggcaccg aagagtatca ggaaaaaagt
     4741 cgtggcgaga gccagacggg aatggaggga gagccaggtc ttttttttga ggaacagtct
     4801 gtcggccaac cagacgcgtg tttccccgat aacgacgaag ccgatgcctc ccaggatgat
     4861 cagcagcata
//
LOCUS       DAOSHE010000116         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15523_length_4864_cov_2.692244, whole genome shotgun sequence.
ACCESSION   DAOSHE010000116 DAOSHE010000000
VERSION     DAOSHE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15523_length_4864_cov_2.692244"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLD82_04475"
     CDS             <1..418
                     /locus_tag="PLD82_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64675.1"
                     /translation="GLVSACDTDDTASKAPVVRIPVKRSTVAELVATLRAAIRHGQHK
                     VVQGLYTQASAAAVQRSIKTGLGTAGSIDETILLEFEHPAAKGKAPIEIIDQQPKSLR
                     LRLPSGNLLQCVLVEEGGLAIDVLASEGGEILLLAE"
     gene            752..2530
                     /locus_tag="PLD82_04480"
     CDS             752..2530
                     /locus_tag="PLD82_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64676.1"
                     /translation="MDELLTCLPGWTRAVVRTTGAKFFSIHPQQAYSVCLVALILGTI
                     EHNGDLLRGHELRVSIAQRSGVITETQESSRKEFNSAFAEAVRSQVERIAARSSITVT
                     VTVGSAFRDPELILADMVTGLTNSAHARLSETVPLQDINVHAYFSVVFGQEAGDLLTL
                     AIEGKKWVEAFALLVDTCQQMDIARWTGLAQTIIPKVVAGDAGDIVSDGLDRIGTVLL
                     DLRGAGMQAMESLFTLCRTVLACAGIHPRTRERALYYAVHAAAHSGRIEEGKTYVARY
                     MELLRTHGPALFPDQFERFRRLIDIRLVAVQALWFNQLDFSAVEPALANDLALFRSLR
                     APLLEAGLGATDDLYARLLGTVGQSHAFAAAMTDDPDMLDLAQLEIEEDLSLLPPGDP
                     FIAQGLSFLHSLLWWRRDLEGARRVLARILDVTEGLDAMLDEAIRPLAGSSDKGFVLL
                     DWLRFAALRQALTGVRPSDGILEILASNPAMTTGYPWNLVAKWTAWLFHATGQLRPAH
                     ALMTSVLATNADTTPLGIMLQTVLEGWDALNRGKNPLEGTYLTNLEHCAGFGPAFAAC
                     IQLRKRVWVAPVDPFEAARFLPYYFS"
     gene            complement(2631..3200)
                     /locus_tag="PLD82_04485"
     CDS             complement(2631..3200)
                     /locus_tag="PLD82_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG64677.1"
                     /translation="MNGPFTRETITTIQTTGKHVYRTPSIRTGLAARQIDLEDFQQEV
                     FRHILAREHNHPGFVAALCATPGLLYDFAKKRALDILRARRTADAAFHEAGPDEYADG
                     TLSHGSLITPHLPGPEEALLRREETGSVRNLLETLPAHERATLALAVFERLKPEAIAR
                     LTGCTIREVYARKSSALRRLGKMVSRRMA"
     gene            3664..3879
                     /locus_tag="PLD82_04490"
     CDS             3664..3879
                     /locus_tag="PLD82_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64678.1"
                     /translation="MAILKKRLWLIIGACMAWVMALLGFSISGCSDSVDDTVMPAYGV
                     PYSTSSTLSSSDSSAGMPGKTAAGNTQ"
     gene            complement(3926..4582)
                     /locus_tag="PLD82_04495"
     CDS             complement(3926..4582)
                     /locus_tag="PLD82_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64679.1"
                     /translation="MKKWSLLLLLSVLVVLPAVSAFSYGYDSYRDYDYTRYAGPNVAL
                     IIVSLGVIALAVLANLFFFMKTGEEKEVWKTRLKLVNKIVWGFLIALISVATVRRMPV
                     LEIGMRGDISILYLIVAILMGLYAIYSIVAHYLFLTTKEDRGCSFVELLDFKAFVIPT
                     IIQVLYYATVFISFLLGLSLIFAEREEGLIVIIAGPVVYRIITELALVLFKIEKNTRK
                     "
BASE COUNT         1001 a         1343 c         1407 g         1113 t
ORIGIN      
        1 cgggctggtt tcggcctgcg ataccgacga taccgcttcc aaggcacccg tggtgcgcat
       61 tcccgtcaag cgctcgacgg tcgccgaact cgtggccacc ctgcgcgcgg ccatccgcca
      121 cgggcagcac aaggtcgtgc agggactcta cacccaggcc agcgccgccg cggtccaacg
      181 ctccatcaag accggactgg gcacggccgg cagcatcgac gagaccattc tcctcgagtt
      241 cgaacacccc gccgccaaag gcaaagcgcc catcgagata atcgaccaac agcccaaatc
      301 gctccggctc cggcttccct ccggaaatct ccttcagtgc gttctcgtgg aagagggagg
      361 gttggccatt gacgtgctgg cctccgaggg cggcgagatt ctccttttgg ccgagtgatt
      421 tcatttcagg ttttttttga tgaacaacct cgggccagtc acacccggcg tgtccgttcg
      481 aaccgttttt gcttgagtcc ctgtggtcat catggcatac tttccgtact tccaatggag
      541 cgcatcccat gacctggcac ctctgtattg acgaaaccgg cgaatttgaa tttctgttaa
      601 aaaaaaacag tacctcccgg attgtgggtg cctgtgtgac caaaaccaga ccgggaggat
      661 ccgcaaggaa ctcaagccgg ttctggacag gcacggtata gaccataacc acttttgcga
      721 gatccgtgac ggacagcgcc gtgaacgatt ggtggatgaa ctgctcacct gtcttccggg
      781 atggaccagg gcagttgtca ggacaaccgg ggccaagttt ttttcgatcc atccccagca
      841 ggcctattct gtctgcctgg ttgcgctcat tctcgggacc atcgagcaca atggtgatct
      901 cctgcgcggt cacgaactcc gcgtctccat cgcacagcgc agtggggtca tcaccgaaac
      961 acaagagagt tcccgcaagg aattcaactc ggcttttgcc gaagccgtca gatcccaggt
     1021 cgagcggatt gccgcgcgct cctcgatcac ggtgactgtg acggtcggga gtgcctttcg
     1081 cgatcccgaa ttgattttgg ccgacatggt cacaggactc accaactcgg cccatgcccg
     1141 cttgtcggaa accgtgcctc tccaggatat caatgtccat gcctattttt ccgttgtctt
     1201 cggtcaggag gccggcgatc tcctgacact ggcgatagag gggaaaaagt gggtcgaagc
     1261 gtttgctctg cttgtcgaca cctgtcagca gatggatatt gcccgatgga ctggtctggc
     1321 ccagaccatt attcccaagg ttgtcgctgg tgatgccggc gatattgtgt cggatgggct
     1381 ggaccggatc ggaaccgttt tgctcgattt gcggggagcg ggcatgcagg ccatggaatc
     1441 cctctttaca ctctgcagga ctgtcctcgc atgtgccggc atacaccccc gtacacgtga
     1501 gcgggctctc tactacgccg tgcatgccgc ggcccatagt gggcggattg aggaaggcaa
     1561 aacatacgtt gccaggtaca tggagttgct gcgcacccat ggcccggcgc tctttcccga
     1621 ccagttcgag cgattcagac ggctgatcga tatccggctt gtggctgttc aggctctctg
     1681 gttcaaccag ctcgattttt ccgccgtcga acctgccctc gccaacgatc tggctttgtt
     1741 ccgttctctg cgtgctcccc tccttgaggc cgggcttggt gcaacggatg atctctatgc
     1801 ccgtctcctc ggaacagtcg ggcagtcgca tgcgtttgct gctgccatga cggatgatcc
     1861 cgacatgctt gatctcgcgc aactcgaaat cgaagaggac ctttcactcc tgccgcccgg
     1921 tgatccattc attgcgcagg gcctgagttt tttgcacagc ctcttgtggt ggcgcaggga
     1981 tcttgaaggt gcccgacgtg tcctggcccg cattcttgat gtgactgaag ggctggatgc
     2041 catgcttgat gaagcgattc gtccgctggc gggcagttcg gacaaaggat ttgtgcttct
     2101 ggactggttg cgttttgccg cactgcgaca ggccctgacc ggagtccgcc cctccgatgg
     2161 aattcttgaa atcctcgcgt ccaatccggc catgacgacg ggctatccct ggaatctggt
     2221 ggccaagtgg accgcctggc tcttccacgc aaccggacag cttcgtccgg ctcatgctct
     2281 gatgacttcc gttctggcaa ccaatgcgga tacaactccg ctggggatca tgctccagac
     2341 tgtgctcgaa ggctgggatg cgctcaaccg gggcaagaat ccgctcgaag gtacgtacct
     2401 gacaaacctt gaacattgcg ccggattcgg cccggctttt gcggcctgca ttcagctgcg
     2461 caagcgggtc tgggtggctc cggttgatcc cttcgaggcg gcccgttttt tgccctacta
     2521 tttttcctga gacacggcag cacttgtccg cctgcgtctg aagcggggag gcttgggctc
     2581 ccgccggacc agtgtcacaa aaaaggaccg gcagcggacg ccatgaccca tcatgccatc
     2641 ctgcgggaaa ccatctttcc cagccggcgc agggcggaac tttttcgggc atagacttcg
     2701 cggattgtgc atccggtcag gcgggcaatg gcttccggtt tgagtctctc aaagaccgcg
     2761 agggcgaggg ttgcgcgttc gtgggcgggc agggtctcga gcaggttgcg cacgctcccg
     2821 gtttcttcgc ggcggagcag ggcttcctct ggaccgggga gatgcggggt gatcaggctc
     2881 ccgtgagaca aggtgccgtc tgcgtattcg tccggtcccg cctcgtggaa ggctgcgtcg
     2941 gcggtgcgcc gtgcgcgcag gatatccagc gcgcgctttt tggcgaaatc gtatagcaaa
     3001 cccggtgtgg cgcagagcgc agccacaaac ccggggtggt tgtgttcccg ggcaagaatg
     3061 tggcggaaga cttcctgctg gaaatcttca agatcgattt gacgtgccgc cagacctgtc
     3121 cggatcgatg gcgttcggta gacatgtttg cctgtggtct ggatggttgt gatggtctct
     3181 ctggtgaatg gtccgttcat tgcgctggct cccctggctg gtttgtgcat gcacagaccg
     3241 ggaccgactt tggtccaggt gcttggatgt acatgatcac cagaattgcc acggggtcgg
     3301 gattacggac tcatgttggc ttccttgttg gtacaatctt ggcgttcttg aacggaacgc
     3361 cctgcttccc ttgtctctat aatacgcgca cattgccaaa attctgctga tttgtccaaa
     3421 aaaatgcttg cgttggccat gcggtcaaaa acggcgaatc cgggagtact gtcgaaggat
     3481 ctgtcatgaa tgaacctccc atcctgccag gcgagcccgg tattgtccag gatgcgcttt
     3541 gatatctgga acagatggct atccggcgac gggcgtccgc acaatccgga aaaattgtcc
     3601 agcgtgagtt gcataaccgg ttgctctgcg gtagtcttga gacagaacgg gggagggaga
     3661 cccatggcaa tcctgaaaaa gagactctgg ctgatcatcg gggcctgtat ggcatgggtg
     3721 atggccttgc tcggtttttc catcagtggc tgcagtgatt cggttgatga tacggtgatg
     3781 ccggcctatg gtgtccctta ttcaaccagc agcacgcttt catcgtccga cagttctgcg
     3841 gggatgcccg gcaagaccgc tgcagggaat acgcaataaa aaagctgtcc cggtatggga
     3901 cagcctggat gaaagaccac acggattatt ttcttgtgtt cttttcgatc ttgaagagga
     3961 ccagggccag ctcggtgatg atccggtaga caaccggacc ggcgatgatc acgatcaaac
     4021 cttcttctct ttcagcaaaa atcagggaaa ggccaagcag gaagctgata aagacggttg
     4081 cgtagtacag gacctggatg atcgtcggga tgacaaaggc cttgaagtcc aggagttcaa
     4141 caaaggagca cccacgatct tcttttgttg tcaggaagag ataatgggca acgattgaat
     4201 agatggcata caaccccatc aggatggcga cgatcagata gaggatgctg atatcaccgc
     4261 gcataccgat ctcaaggacc ggcattcttc tgacggttgc gaccgagatc agggcaatca
     4321 aaaagcccca gacgatttta ttgaccagtt tgagacgggt tttccagact tctttttctt
     4381 cgccggtttt catgaaaaag aagagattgg cgaggacagc cagggcaatg acacccaagg
     4441 acacgatgat caatgcaaca ttcggtccgg catagcgggt atagtcataa tcccggtacg
     4501 agtcgtaccc gtacgagaag gctgataccg caggcaggac gacaagcaca gacaacaaca
     4561 acaacagact ccattttttc atacttcgct ccttgaaaaa tcgtattgca tacactctct
     4621 gcatgctagc atggttatgt cctcaatgca atactcggga gacaatttgg gagaaaaact
     4681 ttttcataca gaaatggttt tggttcgaga caatgatgct ctgtattccg gatcgcggga
     4741 atttccgtgc tgtcgccagc aggaagtaga gtatgaagag gatgccggcc catggatacc
     4801 gtgacttcgc gaggctcacc gaaaacggaa agccgggtcg ctgggccggt tggaggggat
     4861 ttat
//
LOCUS       DAOSHE010000117         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15533_length_4860_cov_4.272425, whole genome shotgun sequence.
ACCESSION   DAOSHE010000117 DAOSHE010000000
VERSION     DAOSHE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15533_length_4860_cov_4.272425"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLD82_04500"
     CDS             complement(<1..1216)
                     /locus_tag="PLD82_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG64680.1"
                     /translation="MKKLSRLPALSNVFYMLSGIVAIVGLAMVPSLVLSLFEDGSMAI
                     VFGGLVVCLGLVAIILRLGNYRPSELTIRDGFFITALSWISLAVLGSIPFVLGGFIPS
                     WTDAFFESMSGFTTTGASILKDIEKLPDSLLLWRSMTHWLGGMGVIALAVAIFPLMGA
                     SGYQLFRAEVPGPTTEKLTPRIRTTAGYLWLVYSGLTLLQIILLLTGGMPLLDSVITA
                     FGTMATGGFSNKNASIAAYDSAYLQYVIIVFMFLAGASFTLHFKALSGRPLSYLRDPE
                     FRFYGLVILGTTMFILLDRFLVQGTPVDEAGVRGTLFSVVSIITTTGFITENYELWPM
                     ASQLVLILLMLIGGSTGSTSGGIKCIRIQISLKYIITELRKLAHQSSIHLVRLGRTAV
                     PESAIPNVIAFVL"
     gene            complement(1213..2559)
                     /gene="trkA"
                     /locus_tag="PLD82_04505"
     CDS             complement(1213..2559)
                     /gene="trkA"
                     /locus_tag="PLD82_04505"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOG64681.1"
                     /translation="MKIIIIGGGAVGTALAKKLAFEKHDLTIIDKDPGVTRVVGETLD
                     VAVITGSGSHINVLERAGIADADMLIAASGTDEVNLVACMLANHAGVRHKIARIRDPE
                     FYTSGSAERTKTEFGINLFIRPESEVADEVARLILRSFASEVFEFENGSLLIACLKVD
                     ESFQQAGVPIRQIGNEDFRRKLRLLAIRRPDKTIIPGGDDSIRHKDLIFLATESRHLS
                     KILSFFGKTDTKLRRIMILGANIIGMEIAERLCAVGLKVKLIDRNRPACLEAAARLPD
                     VDVLHSEKSEIDTMALAGVLETDAFIAVSDDEENNILLNLIARHLGVNRTIALVSRSS
                     YQPFMGSIGIDTSINLQQATANAIVRFVRKGEIVSVASFHGIEAEAIEMALPTGSALV
                     GTMLKDAGIPQGAVLAAIVRDGKARIPVGETVFAEGDKAIVFALPKAIPEIERLFS"
     gene            complement(2556..3452)
                     /locus_tag="PLD82_04510"
     CDS             complement(2556..3452)
                     /locus_tag="PLD82_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOG64682.1"
                     /translation="MRPVFLVILSLLLTTTGSGLAAAGQVKQILLVRQDAALPDRGTI
                     TLYELVQTGWKPTLGPLPALLGKRGTAWGRGPWFAPPRQAPVKREGDGRTPAGLFSLG
                     ILYGYAPNPPVATKLPYTRITRYHYAVNDPRSRHYNKTVDIRKVRRDWQSAGPMARSD
                     RLYPWLLEIAHNQDNPLKDAGTWIYLHPWLSKTTPTEGCTAVSPPDFTRIIQRLDPAR
                     HPRIAVIPADKNGEILFALLGLNGRPAASEASGEITAATINTSLETVALHVLETILPP
                     TSCPVLYPPTARQTPGVKGRSG"
     gene            3772..>4860
                     /locus_tag="PLD82_04515"
     CDS             3772..>4860
                     /locus_tag="PLD82_04515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG64683.1"
                     /translation="MKQGCRLVLCVSIALWGILQPDLHAGHRFSGLVSRIDALRARLG
                     IPGCSLAVGYGDGSIEAVGLGFADLEQNIPAGPETQYHIASLTKGFAGLIALQLEAEG
                     RLDLGMVMPDPQRFPDPETSIARVLSHTADRSDGGFVYRSDLFALLTPVFESAARQSW
                     PALLDERILAPLGMTMTVPGRKYAPLQNRLVSLARPYLVNGRETRMVALPSPECNTAY
                     GLVSTVRDLVVWSQALDSGRLVRPSQAGRLFTEKKDQRGNDNPYGLGWFVSEVAGVRL
                     HWHYGWRVPGYGAILVRVPRYGLTFVILANSDRIVSGATLAWGNPLRSIFVVEFVRRY
                     LLAGRDGDPYPPIDPDWDEKEMEHHLGRR"
BASE COUNT          943 a         1327 c         1583 g         1007 t
ORIGIN      
        1 gcaacacaaa ggcgatgacg ttggggatgg ccgattcagg gacagccgtg cggccgagcc
       61 ggaccagatg gatgctgctc tggtgtgcca gtttccgcaa ttccgtaatg atatatttga
      121 gcgagatctg gatccggata cacttgatgc ctcccgatgt cgagcctgtg gatcccccga
      181 tcagcatgag cagaatcaaa acgagctgtg aagccattgg ccagagttcg tagttttcgg
      241 tgatgaatcc cgttgtcgtg atgatcgaga caacactgaa gagggtgccc ctgactcctg
      301 cttcgtcaac cggggtaccc tgtaccagaa atctgtcgag caggatgaac atcgttgttc
      361 cgaggatgac cagtccgtaa aaccggaatt cgggatcgcg caggtaggac agtggccggc
      421 cggagagggc tttgaagtgc agggtgaagc tggctccggc caggaacatg aagacgatga
      481 tcacgtattg caggtaggcg gagtcgtagg ccgcgatact ggcattcttg ttcgaaaaac
      541 cgccggtggc catcgtgccg aacgccgtga tgaccgaatc caacagcggc attcctccgg
      601 tcaggaggag gatgatctgg aggagggtca gccccgagta gaccagccag aggtagccgg
      661 ccgttgtccg gatcctcggg gtgagttttt ccgttgtcgg accgggaact tcggcccgga
      721 aaagctgata cccgctggca cccatcaggg gaaagatggc cacggccagc gcgatgactc
      781 ccatgccgcc cagccaatgg gtcatgcttc tccagaggag gagactgtcc gggagttttt
      841 cgatgtcctt gaggatggaa gctccggttg tggtaaagcc ggacattgat tcaaaaaacg
      901 catcggtcca ggaggggatg aaaccgccga ggacaaaggg aatggatccc aggacggcca
      961 gggagatcca ggagagcgcg gtgatgaaga atccgtcgcg gatggtgagt tcgcttggtc
     1021 ggtaattccc gagccgcaag atgatggcca cgagtccgag gcagacgacc agccccccga
     1081 agacgatggc catcgatccg tcttcaaaca ggctgaggac cagggaaggt accatggcca
     1141 agccgacaat cgccacgatc cccgagagca tgtagaacac attggaaagg gccgggagtc
     1201 tggagagctt tttcatgaga aaagccgttc gatctcgggg atggccttgg gcagggcaaa
     1261 gacgatggcc ttgtcaccct ccgcgaaaac tgtttcacct accgggatcc tcgcctttcc
     1321 gtcgcggaca atggccgcca ggacggcgcc ctgggggatc ccggcatcct tgagcatggt
     1381 gccgacgagg gcgctccctg tcgggagtgc catttcgatc gcctccgctt cgatgccgtg
     1441 gaaactcgcg accgacacga tctcgccttt acggacaaaa cggacaatcg cattggcggt
     1501 ggcctgctgg agattgatgc ttgtgtcgat gccgatggat cccatgaagg gctggtagga
     1561 ggagcggctg acgagtgcga ttgtccggtt gacaccgaga tgcctggcga tcaggttgag
     1621 gaggatattg ttctcctcgt catccgaaac cgcaatgaag gcatcggtct cgaggacacc
     1681 ggccagggcc atcgtatcga tttcgctttt ttcgctgtgc agcacatcca cgtcgggcag
     1741 gcgcgcagcc gcctcgaggc aggccggccg gttccggtcg atcagcttga ccttgagccc
     1801 gacggcgcag agacgctcag cgatctccat cccgatgatg tttgccccga ggatcatgat
     1861 cctcctgagc ttcgtatcgg tctttccgaa aaacgagaga attttggaga gatgccggct
     1921 ttcggttgca agaaagatca gatccttgtg cctgatgctg tcgtctccgc cagggatgat
     1981 cgtcttgtcc ggtcggcgaa tcgcgagcag ccggagtttg cgccggaagt cttcatttcc
     2041 gatctggcgg atcggaactc cggcctgctg gaaggactca tcaaccttga gacaggcgat
     2101 caggagcgag ccgttctcga attcgaagac ctcggatgcg aaggagcgca ggatcaggcg
     2161 ggcgacctcg tcggcgacct cactttcggg gcggataaag aggttgatcc cgaattcggt
     2221 ttttgtccgc tcggccgaac cgctggtgta aaactccgga tcgcggatcc gtgcgatctt
     2281 gtgccggact ccggcatgat tggccagcat gcaggcgaca agattgactt cgtctgttcc
     2341 gctggccgcg atcagcatgt ccgcgtcagc gataccggca cgctcgagga cattgatatg
     2401 cgagccgctt ccggtgatca ccgcgacatc cagagtctcg ccaacgaccc gggtcacgcc
     2461 cggatccttg tcaatgatcg tcagatcgtg tttttcgaag gcgagttttt tggccagggc
     2521 tgtcccgacc gcccctccgc cgatgatgat gatcttcatc ccgaacgtcc tttcacgccg
     2581 ggagtctggc gggcggtcgg agggtagagg actggacaac tggttggcgg gaggatcgtt
     2641 tccagaacat gcagggccac tgtctcgaga gatgtgttga tggttgcggc cgtgatttct
     2701 ccggatgcct cgcttgccgc cggacgcccg ttcagtccga ggagtgcaaa gagtatttct
     2761 ccgtttttgt cggcggggat gaccgcgatc cggggatgcc tggccggatc gagtcgttgg
     2821 atgatcctgg tgaagtccgg cggggagacg gctgtgcacc cttccgtcgg tgttgtcttc
     2881 gagagccagg gatggaggta gatccaggtc ccggcatctt tgagcgggtt gtcctggttg
     2941 tgggcgatct cgaggagcca gggatagagc cggtcgcttc gggccatcgg acccgcgctc
     3001 tgccagtccc ggcgcacctt gcggatatcg acggtcttgt tgtaatgacg ggaccgggga
     3061 tcgttgacgg cgtaatggta gcgcgtgatc cgggtatagg gcagctttgt tgcgacggga
     3121 gggttcggag catagccgta gagtatcccg agggaaaaga gcccggccgg ggtccgtccg
     3181 tcgccctccc gtttgaccgg agcctggcgg ggcggtgcaa accagggtcc ccgcccccaa
     3241 gccgtcccac gcttcccgag gagggccggg agcggcccca gggtgggttt ccagccagtc
     3301 tggacgagtt catagagggt aatggttccc cgatcgggaa gggcggcatc ctgccggaca
     3361 agcagaatct gttttacctg gccggcggct gccaggcccg agccggttgt cgtcagaagg
     3421 agcgagagta tcacgaggaa tacgggacgc atatcaggta ctatatcgga aaactcgatc
     3481 catgggaaga gggttgaaca cgcgcgctac gcagggcaat gggcgaagtt tccgtacgag
     3541 acatgcaatc ccggattacg caatttgctt aaaatttagg caccgcaccc ggctgggtat
     3601 aggtgaagtc cccgaagggg gctggttcac tctgggctgg gattgggggc cgtttttgta
     3661 catacaaacc ggcctccgca aaaaagcgcg gcatcgccca ggccgcaggg atcaacatgc
     3721 tgtattgtcc ggtaatatcc tgctagactg gcatgagcgt gggaggtgat gatgaagcag
     3781 gggtgccggc ttgtcctgtg tgtcagcatc gccctgtggg ggattttgca gcccgacctg
     3841 catgccggtc accgtttcag tggtctggtc tcccggattg atgctttgcg tgcccgtctc
     3901 ggcattcctg gatgtagtct ggctgtcggg tacggggacg ggagtatcga ggctgtcggg
     3961 ctgggttttg cggatcttga gcaaaacatc cccgccggac cggagacgca ataccatatc
     4021 gcttcgctca caaagggctt tgccggtctg attgccctcc aattggaagc cgaggggcgg
     4081 cttgatctcg gaatggtcat gccggatccc caacgcttcc cggatccgga gacgagcatc
     4141 gcccgggttc tttcccatac cgcggaccgg agtgacggtg gctttgtcta ccggtccgat
     4201 ctgtttgccc tcctgacgcc ggtcttcgaa tcagcggcgc gccaatcctg gccggctctt
     4261 cttgatgaac ggattctcgc tccacttggg atgacgatga ctgtccccgg gcgcaagtat
     4321 gcgccgcttc agaacaggtt ggtctccctg gccaggccat atctggtgaa cggacgggaa
     4381 accaggatgg tggccctccc gtctccggaa tgcaataccg cctacggtct tgtctcgaca
     4441 gtgcgcgatc ttgtggtctg gtcgcaggcg cttgattccg ggcgccttgt ccgcccctct
     4501 caggccggga gactgttcac agaaaaaaag gaccaaagag gaaacgacaa tccatacgga
     4561 ctgggctggt ttgtcagcga ggtggccggt gtccggctgc attggcatta tgggtggcgg
     4621 gtgccgggct atggcgccat cctggtccgt gtcccgcgat acggcctgac attcgtgatc
     4681 ctggccaatt cggaccggat cgtttcgggg gcaacgctgg cctggggaaa cccgctccgc
     4741 tccatttttg tcgtggaatt tgtccggcgc tatctcttgg caggtcggga cggggatccg
     4801 tatccgccca ttgatccgga ctgggacgag aaggagatgg agcaccatct cggacggaga
//
LOCUS       DAOSHE010000118         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15840_length_4795_cov_3.810127, whole genome shotgun sequence.
ACCESSION   DAOSHE010000118 DAOSHE010000000
VERSION     DAOSHE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15840_length_4795_cov_3.810127"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLD82_04520"
     CDS             <1..909
                     /locus_tag="PLD82_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG64684.1"
                     /translation="TAAGWKIDYVEYYVTSSSSSSSSSSVSSSSSSVSSSSSSSATPG
                     SFQEDFSLSQSIRDYYRSAYGLSGSALKTKLQQIITAGYVTKSYSYLYTIYADADRTS
                     DSKVWDIYSDRDGTGLNRPYTYSFGTGTCGTYSGEGDCYNREHMIPQSKFGEKSPMVS
                     DAHHVLPTDGKVNGMRSSYPHGAVSSATWTSQNGSKLGSGTSASGYTGTVFEPINAYK
                     GDTARIYFYFCVRYYNNSAIQAWESMNTGAKLKTWAQTLYRQWASADPVSAKEVARNN
                     GVYLHQKNRNPFVDYPVLCNMIDFTN"
     gene            complement(1301..1852)
                     /locus_tag="PLD82_04525"
     CDS             complement(1301..1852)
                     /locus_tag="PLD82_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG64685.1"
                     /translation="MELKQILDKRRSLRVYDTRIPDRETLLSCIEAARISPSGENVQP
                     WRFCVIDEPGLLGELTEACTGGIYLPTKFIRKAPVIIAVTAKTDIMVNKIGTFLQGTQ
                     FYLLDLGIACQQLVLRAEDLGLGTCYIGWFHVEKARKVLDLPKKEKLVCLISLGYPPP
                     GYKSPEGHKRKALDEIVRFNRED"
     gene            1937..3586
                     /locus_tag="PLD82_04530"
     CDS             1937..3586
                     /locus_tag="PLD82_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG64686.1"
                     /translation="MTASGYLRCGVLGLMASGEFVVEVRDTTLVWLLTGGGLLFGLLC
                     LAGRRFVPKQTWLRDVGIVFTLWAVFMGGWNCLLGGYQERDLAAHHWLVRDCTGHVRE
                     MVVKEKRTRLILEIDQALLINGQKKALSGCLVMFLENGSVAGVTNGSLVRVIGDVLPL
                     PEGGQWAGFWKFLADRDVRGLIRRPEHLELLSGPADLRLALRTLRQHCDSWFESLGDT
                     GMAFMKALVFGDRSGLGQEETKDFVDAGIIHVMAVSGMHIAVASAVLFWLARLVGVGR
                     RFAWPCVMAILVIYAGLCGWTASVMRAVLMLGLLQIWKLVRSRVSLLDLLIVVAGLLW
                     LEDRTVLGQPGYLLSFAATAGIILFAKPLTEFLQWLRLPRWLASTLGVSLAAQLAVIP
                     ILAGFFGRISLVSPLANCIFVPVILGLQAVGLVLSLAAFFGAGAWVASFFGTMIDLLY
                     AAIHQAATLPWASLEIPEFPWWGVLAYTCLITGIIKGSERAVLGRRKNRGLALATQVA
                     SIFAREGDDGPARSGAEFSCQPAHPRAHCRYRSGSTRRRCA"
     gene            3483..4379
                     /gene="rsmA"
                     /locus_tag="PLD82_04535"
     CDS             3483..4379
                     /gene="rsmA"
                     /locus_tag="PLD82_04535"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG64687.1"
                     /translation="MTARHGLGQNFLVSRHILERIVATAAVQPGEDVLEIGPGLGFLT
                     HFLLSAGARVRAYETDAALCDWLAGVFHGVPAFDLVRGDFLQADCSLYGASMDGAGDG
                     NRPEDVPQKKAAVAHARVIANLPYNITTPVFDRLFSLPAITEIIVMVQRQVAERIVAK
                     PATREYGALTLFAGFHSEPELLFHVSPGNFHPAPSVYSSVVRLRIRREKPLAGEAARL
                     FFALGRAVFSSRRKVLRNSLRESPFCGLNGEQADRLLELAGILGGIRGEEMSMDEFVV
                     MARAWESSGLPRPRVAREAERG"
     gene            4372..>4795
                     /locus_tag="PLD82_04540"
     CDS             4372..>4795
                     /locus_tag="PLD82_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG64688.1"
                     /translation="MDDALPEVIVHALAAWFRDHGRPYFFRRHPTAYSVWILEVMSQQ
                     TQIARAEERHRRWLEHFPDIQALAAASESEVLREWEGLGYYSRARNIHKAARQVLDAG
                     GEMPATRDELIRLPGIGAYTAASIASIVHGEPVAALDAN"
BASE COUNT          869 a         1371 c         1459 g         1096 t
ORIGIN      
        1 actgctgccg gctggaagat cgactacgtc gagtactatg tcacatccag ctcaagcagc
       61 tcgtccagct ccagcgtttc ttcttcaagc tcttcggttt cttcaagctc cagctcctca
      121 gcaacacccg gcagcttcca ggaagatttc tcgctttcac agagcatccg cgattattat
      181 cgcagcgcct acggtctctc gggttcggcc ctcaagacca agctccagca gatcatcacg
      241 gccggatatg tgacaaagag ctatagctac ctttacacga tctacgctga tgcagaccgc
      301 acctccgaca gcaaggtttg ggacatctac tcggaccgcg acggaaccgg actcaaccgg
      361 ccctacacct actccttcgg gacagggacc tgcggaacct acagtggaga aggcgattgc
      421 tacaaccgtg agcacatgat tccccagagc aagttcggcg agaagagccc gatggtcagc
      481 gatgcccacc acgtcctccc gacggacggc aaggtcaacg ggatgcgctc cagctatccg
      541 cacggtgcag tttcctcggc cacctggacc tcacaaaacg gctccaagct cggttccggc
      601 acctcggcct ccggctacac cggcacagtc ttcgaaccca tcaacgccta caagggcgac
      661 acagcccgca tctacttcta cttctgcgtg cgctactaca acaactcggc catccaggca
      721 tgggagtcca tgaacactgg tgccaagctc aagacctggg cacagacgct ctatcgccag
      781 tgggcttcgg ctgacccggt cagtgccaaa gaagttgccc gtaacaatgg tgtgtatctg
      841 caccagaaaa accgcaaccc ctttgtggac tacccggttc tctgcaacat gatcgacttc
      901 accaactgat caggcaggat cagcagtcaa agaccgctcc ttcgggggcg gttttttgtt
      961 tgatgcgccc gtgactccag ctggggcact gcaggggcgc gtactctctc gtgatgtggt
     1021 tgaaccgact gatcaggaaa cggggctcca ttgttgtgag acagttgtaa caggtatatc
     1081 gacatcccgc cacaaaccgg gacgctccgg agacagatca ccttgttggg tgtccgggcc
     1141 aaagatacgc aagcaaccaa ccggaacagg atgggaggtc tgactcgggg cacggaagcc
     1201 ggttgagcac aaggttcact tgcacagaac cgggcacatg ccattcggga gaggccattc
     1261 agaaaaagaa accggatgag ggagagatcg tgagcggttt tcagtcttcg cggttgaaac
     1321 ggacgatctc gtccagggct ttccgcttgt gaccctcggg gctcttgtac ccggggggcg
     1381 gatatccgag cgagatcagg caaacgagtt tttctttttt gggaagatcg agtaccttgc
     1441 gtgccttttc cacatggaac cagccgatgt agcacgtacc caacccgagg tcctctgccc
     1501 ggaggacaag ctgttggcag gcgatgccga ggtccaggag gtaaaactgg gttccttgga
     1561 ggaatgtccc gattttattg accatgatgt cggttttggc tgttaccgcg atgatcaccg
     1621 gggctttgcg gatgaatttg gtgggaagat agattccacc ggtacaggcc tcggtcaact
     1681 cgccgagcag cccgggttcg tcgatcacac aaaagcgcca gggttggacg ttttcaccgg
     1741 agggtgagat ccgggctgct tcgatgcagg agaggagcgt ttcccggtcg ggaatccgtg
     1801 tatcatagac ccgcaggctt cggcgtttgt caagaatctg cttcagttcc atgcttgacc
     1861 tcattatttt ggaatttttt gcgcaccatt gaacgtccgg cgactcttct ccctgtagaa
     1921 gatacggagg aagtcaatga cggcaagtgg atatctgcgg tgtggcgtgc ttggtttgat
     1981 ggccagcgga gaatttgtgg ttgaagtcag ggatacgaca ctcgtctggc tcctgacggg
     2041 cgggggcctg cttttcggtc tgctctgcct ggcgggaagg cggtttgtcc caaagcagac
     2101 atggctgcgg gatgtgggta tcgtgttcac cctctgggcc gtgtttatgg gtggctggaa
     2161 ttgcttgctc ggagggtacc aggagcggga tctggcagcc catcactggc ttgtccggga
     2221 ctgcacgggt cacgtccggg agatggttgt caaggagaag cggaccaggc tgattctgga
     2281 gatcgatcag gccctgctca tcaatggaca gaaaaaagcc ttgtccggtt gcctcgtcat
     2341 gtttttggag aacgggtccg tggccggggt gacgaatggc tccctcgtca gggtgatcgg
     2401 ggatgtgctc cctttgcccg aaggtggtca atgggccggg ttttggaaat ttctcgcgga
     2461 ccgggatgtt cggggtctga ttcggcgacc ggagcatttg gaacttcttt caggcccggc
     2521 agatttgcgg ctggcactca ggactctccg gcaacactgc gattcctggt ttgagtcatt
     2581 gggagatacg gggatggcct ttatgaaggc actggtcttt ggtgaccgga gcggtcttgg
     2641 gcaggaagag acaaaggatt ttgtcgatgc cggcatcata cacgtgatgg ccgtctcggg
     2701 catgcatatt gccgttgcca gtgccgttct gttctggctg gcccggcttg tcggcgtggg
     2761 ccggcgattt gcctggccct gtgtgatggc catcctcgtc atctatgccg ggctctgtgg
     2821 ctggacagct tccgtcatgc gggccgtcct catgcttggg ctgctccaga tctggaagct
     2881 cgtccggtcc agggtttccc tgctcgatct tctgattgtc gtggccggac tcctctggct
     2941 ggaggaccgg acagtccttg gacagccggg gtacctcctg tcttttgccg cgacagccgg
     3001 catcatcctc tttgcaaaac ctttgaccga atttctgcaa tggttgcgcc tgccccgctg
     3061 gctggcctcg acccttggcg tctctctggc ggcccagttg gccgtcattc cgatcctggc
     3121 cgggtttttc gggcggatct ccctggtctc acctttggcc aattgcatct ttgtcccagt
     3181 cattctcggt ttgcaggccg tcggtttggt cctgtccttg gccgcgtttt tcggtgccgg
     3241 cgcctgggtt gcttcatttt tcggaaccat gatcgacctt ctctatgcgg cgatccatca
     3301 ggcggccacg ctcccctggg ccagcctcga gattcccgaa tttccctggt ggggggtgct
     3361 ggcgtatact tgtctgatca cgggcatcat caaggggagt gaacgtgcag tcctgggacg
     3421 tcgaaaaaat cgaggccttg ctctcgccac gcaggttgca agcatttttg cgcgagaggg
     3481 ggatgacggc ccggcacggt ctggggcaga attttcttgt cagccggcac atcctcgagc
     3541 gcattgtcgc taccgcagcg gttcaacccg gagaagatgt gcttgagatc ggtcccggtc
     3601 tcggcttttt gacgcacttc ctcctctcgg ccggcgcccg ggtgcgggcc tatgaaacgg
     3661 acgcggcctt gtgtgactgg ctggccgggg tttttcatgg ggttcccgcc ttcgatctgg
     3721 tccgtggtga tttcctccag gcggactgct cactctatgg tgcttccatg gacggcgctg
     3781 gcgatggaaa ccggcctgag gatgttcccc aaaaaaaagc agcggttgcc cacgcgcgcg
     3841 tcatcgccaa cctgccgtac aatatcacga cacccgtttt tgaccggctc ttttccctcc
     3901 cggccattac cgagatcatt gtcatggtcc agcgacaggt ggccgaacgg attgtggcca
     3961 aaccggcgac ccgcgagtat ggggcattaa ccctttttgc cggcttccac tccgagcccg
     4021 agctgctctt tcatgtctct ccgggaaatt tccatccggc tccatccgtc tattcgtccg
     4081 ttgtcagact gcggatccgc cgggaaaaac ccctcgccgg tgaagcggcc cgtctcttct
     4141 ttgccctcgg tcgggcggtc ttttcatccc ggcgcaaggt gttgcggaac agtctgcgtg
     4201 aatccccgtt ttgcggtttg aacggggaac aggccgaccg tctgctggag ctggccggaa
     4261 tcctgggcgg aatccggggt gaagaaatgt cgatggacga atttgtcgtc atggcacgcg
     4321 cctgggaatc aagcgggctt ccccgccccc gggttgcccg ggaggcggag cgtggatgat
     4381 gcgctcccgg aggtgattgt ccacgcactg gctgcctggt tcagggatca cgggcggcca
     4441 tattttttca ggcggcatcc tacagcctat tcggtctgga ttctcgaggt gatgtcccag
     4501 cagacgcaga ttgcccgggc cgaggagcgc caccggcgct ggcttgagca ctttcccgat
     4561 attcaagcgc tggcggccgc gtcggagtcc gaggtcctca gggagtggga gggcttgggc
     4621 tattattccc gcgcccggaa tatccacaag gccgcccggc aggttcttga tgcagggggc
     4681 gagatgccgg cgacgcggga tgaactgatc cgcttgcccg ggatcggggc ctataccgcg
     4741 gcctcgattg cgtcgatcgt gcatggcgaa ccggtggccg cactggatgc caatg
//
LOCUS       DAOSHE010000119         4777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_15935_length_4777_cov_4.316815, whole genome shotgun sequence.
ACCESSION   DAOSHE010000119 DAOSHE010000000
VERSION     DAOSHE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15935_length_4777_cov_4.316815"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..2616
                     /locus_tag="PLD82_04545"
     CDS             214..2616
                     /locus_tag="PLD82_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG64689.1"
                     /translation="MSATHGFSEPLLRAIVESLEDIIFHIDPAGLIRSWHAPSPTDLY
                     TNPAEFEDKHLADVLPAELADRFATAFERAKKTGCETLEYSLEMTGRIHWYLAHITPL
                     DDGGQILVIRNTTRLREYAHALETRGAQMDAQFEILPLATVVFQSFSGGLIFQRYNRA
                     TRRLVGDGIASLTGRPAHEIFAGHPEIVANLGACIREKTSFTLEMEFPWPGMSNPKNV
                     HADFVFVPPDMVMLHLTDADTVRRATQQARIAEFVVESAIEGIVLTDASGNIERVNPA
                     FTTITGFTADEAVGKNPRILKSDRHDEKFYEEMWHTLITTGRWEGEIWNRRKNGETYP
                     ERMVIAAIRDQNGLLRHYVSVFTDISDVKSRDERIAHQAMHDALTDLPNRNLLTDRIR
                     YALSRLQHNSTPTLPLLFIDIDHFKYINDAHGHIVGDYLITSIARRLEELLPEGAVLS
                     RVGGDDFIILLPPARSTTEIMSFVVSVSAAFRESFSVGKEEFFLTPSIGVSVAPGDGA
                     DEISLIKNAELAMYHAKKSGRNTWQFYSSEMNREATRRIELQGLLRKALDDGQYELWF
                     QPVVDSLTNEVVSAEALVRWRDPARGIISPGEFIPVLEENGQIVPLGAWILEQSLLAA
                     AQWQSSLPGVALSVNLAARQFSEPDFIVSVFRTLNKSGLDPALVALEITENTIMSDRS
                     RVSGFMEQIKKMGLRISIDDFGTGYSSLAYLKRFDADYLKIDRSFVIDLPHDRDSSAI
                     AEAVIALARSLGLTTIAEGVENEEQMRFLRERGCTRIQGYLYSKPLPLPEFIEFAKLR
                     SR"
     gene            2623..3405
                     /locus_tag="PLD82_04550"
     CDS             2623..3405
                     /locus_tag="PLD82_04550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG64690.1"
                     /translation="MYADAHAHLHDQRLRTTNRPGTVRSRAGAAGLDLICAASVSPSD
                     WMDLVRFLEAGTGEPCESGLAILPSFGLHPAFDTGDYDALRDGIPSNGPRPRAIGEIG
                     LDQTVDTPMEWQCERLEMMLALASSRNLPVILHARGPWDRPFCVLDRYPGVHGIMHAF
                     GGAPEIARECIRRGYLLSAGPRLLDSGAKKMRASFAEIPLPSIVLESDAPDMCPIDET
                     PPNEPSVIVRVAETLAKIRGESAEKIAVETRRNLLRLFDTMA"
     gene            complement(3587..4681)
                     /locus_tag="PLD82_04555"
     CDS             complement(3587..4681)
                     /locus_tag="PLD82_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG64691.1"
                     /translation="MKSVLSSAILVLVLASHPLWSSGNPATNQIPRSPLHGEFRIIPF
                     AFYTPETSVALGAVPMYVFRLGDEDLRPSTINAILIATVKAQFTGTIVGDFWFPGGDE
                     FRVQLTGLRYPGEFFGIGNDNRPDASESYLQYEARGVFEYNHFFMPSLSVGARAEFSG
                     YETADIESGRLLASGSIPGWSGSFVSGFGLALAWDTRDNVFSAREGVWFQTALTRYDG
                     IFGSGREYSHFVLDLRGYGTLAGRHTFAGQLLAEIQDGDVPFHRLAGLGGSKLLRGAF
                     EGRLRDKALLAAQFEYRLHLSDRWTVNAFGHTGTVASEAGLLSTGDLHFGAGFGARYI
                     FLPRERLAFRLDIAWTAGDFGFYFLSLEAF"
BASE COUNT         1075 a         1568 c         1269 g          865 t
ORIGIN      
        1 acagtggaag ttctgacccc gccatcacaa gtgtacccgg cctcaccccg aaacgggtgg
       61 ggctttgttc acagcctccc ttgccaggcg cctttctctt gacaaaaccg ggtttcatgc
      121 cggatactcg cagtgacgag ttaacgttac cgggcgcaac acgcaaggtc ttccgcttct
      181 atggttgtcc cccgcaatta ccgggaaggt cagatgtctg caacacatgg tttttccgag
      241 cccctcctcc gcgccatcgt cgaatccctg gaagacatca tcttccacat tgatcctgcc
      301 ggcctcatcc ggtcctggca tgcaccctcc ccgaccgatc tgtacaccaa cccggccgaa
      361 ttcgaagaca aacatctcgc ggatgtcctg ccggctgaac ttgcagaccg gtttgccact
      421 gcctttgagc gggccaaaaa aaccggatgt gaaaccctgg agtactctct cgaaatgacc
      481 ggccggatac actggtatct ggcccacatc acccccctgg atgacggcgg acagattctc
      541 gtcatccgga acacgacccg ccttcgggaa tacgcccacg cccttgaaac ccggggcgcg
      601 cagatggatg cccagttcga gatcctcccc ctggcgaccg tcgtgttcca gtccttttcc
      661 ggtggactca tctttcaacg ctataaccgg gcaacccgcc gcttggtcgg cgacgggatc
      721 gccagcctca ccggccgacc ggcacacgag atttttgccg ggcaccccga gatcgtcgcc
      781 aatctggggg cctgcatccg tgagaaaaca tcctttaccc tcgaaatgga gtttccctgg
      841 cccgggatga gcaaccccaa aaatgtccat gccgatttcg tcttcgtccc gccggacatg
      901 gtcatgctgc acctcacgga tgcggacacc gtccgccggg cgacacagca ggcccggata
      961 gccgagtttg tcgtagaatc cgccattgaa ggaatagtcc tgacggatgc ttccggaaac
     1021 atcgaacggg tcaacccggc attcaccacc atcacgggct tcaccgcaga cgaggccgtc
     1081 gggaaaaacc cccggatcct caaatcggac cgtcacgacg aaaaattcta cgaagagatg
     1141 tggcacaccc tcatcacgac aggtcgctgg gaaggtgaaa tctggaaccg gcgaaaaaac
     1201 ggcgagacat acccggagcg gatggtgatc gccgccatca gggaccagaa cggacttctc
     1261 cggcactacg tttcggtctt caccgacatc tccgacgtca aatcccggga cgaacgaatc
     1321 gcccatcaag ccatgcacga tgccctgacg gatcttccca acaggaatct cctcaccgac
     1381 cgcatccgct acgccctctc acgcctgcaa cacaacagca ctccgacact cccgctgctc
     1441 ttcatcgata ttgaccattt caaatacatc aacgacgcac acggccatat cgtcggcgat
     1501 tatctcatca cctcgatcgc ccgccggctt gaggagctcc tcccggaggg ggcggttctc
     1561 tcacgagtcg gtggtgacga cttcatcatc ctgctccccc cggcacggag cacgaccgag
     1621 atcatgtcct ttgtcgtctc agtctccgcg gccttccgcg aatccttttc cgtcgggaag
     1681 gaggagtttt ttctcactcc aagcatcggc gtcagtgttg ccccggggga tggagcagac
     1741 gagatcagcc tgatcaaaaa cgccgaactc gccatgtatc acgccaaaaa atccgggcga
     1801 aacacctggc aattctactc ctccgagatg aacagggagg ccactcgcag gattgaactc
     1861 cagggtctcc tgcgcaaagc cctcgatgat ggccagtacg aactctggtt ccagcctgtt
     1921 gtcgactcac tcacgaacga agtcgtcagt gctgaagccc tggtacgctg gcgcgatccg
     1981 gcccggggca tcatctcacc cggagagttc attccagtcc tcgaagaaaa cggccaaatc
     2041 gtcccgctgg gagcgtggat cctcgaacaa tccctgctgg cggcagcaca atggcaaagc
     2101 tcactcccgg gtgtagcact ctcggtcaat ctcgccgcca gacaattttc tgagcctgac
     2161 tttatcgtct ctgtcttcag gaccctcaac aagagcggac tggatccggc actggtcgcc
     2221 ttggagatca ccgaaaacac gatcatgtcc gaccggagcc gggtcagcgg cttcatggag
     2281 cagatcaaaa aaatgggatt gcggatctcg atcgatgact tcggaacagg ctattcgtcc
     2341 ctggcctatc tcaagcgttt tgatgcggat tatctcaaaa tcgaccggag cttcgtgatc
     2401 gacctccccc atgacaggga cagttcagcg atagcggagg ccgtcatcgc cctcgcccgc
     2461 tccctgggac tgacaacgat cgccgaaggt gttgaaaacg aggagcaaat gcgattcctc
     2521 cgtgaacgcg gttgcacgcg tatccagggg tatctctaca gcaagccact cccgctcccc
     2581 gagttcatcg agtttgcgaa actccgttcc cgttgaacaa ccatgtacgc ggacgcccac
     2641 gcacatctcc atgaccagcg ccttcgtaca accaatcgcc cgggaaccgt ccgttccaga
     2701 gccggagcag ccgggctgga cctgatctgc gccgcgtccg tcagcccctc cgactggatg
     2761 gacctggtcc gctttctgga ggccgggacc ggcgagccgt gcgaatccgg ccttgccatc
     2821 ctgccgtcct tcggcctgca ccccgccttc gacacgggag attacgacgc actccgtgac
     2881 ggcatcccct ccaacggacc gcgcccccgg gcgatcggag agatcggcct ggaccagacc
     2941 gtcgacacac ccatggagtg gcaatgcgaa cgcctcgaaa tgatgcttgc cctggcgtcc
     3001 agccggaatc tcccggtcat cctgcatgcc aggggcccct gggacagacc cttttgcgtc
     3061 ctggaccggt accccggagt ccacggaatc atgcatgcct ttggcggggc ccctgaaatc
     3121 gcccgggagt gtatccgccg gggctatctc ctctcggccg gcccccggct gctcgattcc
     3181 ggtgccaaaa aaatgcgggc cagttttgcg gagatcccgc taccatcgat tgtcctggag
     3241 agtgacgcgc cagacatgtg cccgatcgac gaaactccgc ccaacgagcc ctctgtgatc
     3301 gtccgggttg ccgaaacact ggcaaagatc cgtggagagt ccgcggagaa gattgctgtc
     3361 gaaacacgcc gcaatctgct tcgcctgttt gacacaatgg cataacaatc ttttcgggga
     3421 acaacctcca gccggggaga ggcagtctgt cctgatcagc accgctcacg agcaagtgtc
     3481 aggcggaaga gcattggcaa aaacggacag gaacgcacaa cacggcgcat accactcaac
     3541 gggaggccaa ctcccggaga ggggcacccg acggatgcac catttctcaa aacgcttcga
     3601 gcgacaaaaa atagaacccg aaatccccgg ccgtccaggc aatatcgaga cggaaagcca
     3661 gccgctcccg gggaaggaag atgtagcgcg ccccgaaacc ggctccaaaa tgcagatctc
     3721 ccgtcgagag aagtcctgcc tcgctggcga cggtcccggt gtgtccgaag gcgttgacgg
     3781 tccagcggtc actcaagtgc agccgatact cgaactgggc cgccaggagc gccttatccc
     3841 gcaaccggcc ctcaaaagcc ccccgcagga gctttgaacc acccaacccg gccaagcggt
     3901 gaaacggaac atccccgtcc tggatttccg cgagaagctg tccggcaaac gtgtgccgtc
     3961 cggccagagt accatacccg cgcagatcaa ggacaaagtg ggagtactcc cgcccactcc
     4021 cgaagatccc gtcgtagcgc gtcagggccg tctggaacca gacgccctcc cgcgcggaaa
     4081 agacattgtc cctggtatcc caggccaagg ccagtccgaa gccactcaca aacgaaccgc
     4141 tccaccccgg aatgctccca ctggccaaaa gccttccgga ctcgatgtca gccgtctcat
     4201 acccggaaaa ttccgcccgt gctccgacac tgaggctggg catgaaaaag tgattgtact
     4261 cgaagacccc ccgggcttcg tactgaagat aggattcgct ggcatctggc cggttgtcgt
     4321 tcccgatacc gaagaactct ccggggtagc gcagcccggt aagttggacc cggaattcat
     4381 ccccgccggg gaaccaaaaa tctccgacga tggtcccagt gaactgggcc ttgacggtgg
     4441 cgatcagaat cgcattgatg gttgacggcc gcagatcttc atcgccaaga cggaaaacat
     4501 acatgggaac ggctccgagc gccacggatg tctccggagt ataaaacgca aaaggaataa
     4561 tgcggaactc gccatgcaag ggcgacctcg gaatctggtt tgtcgccgga ttaccgctgg
     4621 accaaagcgg gtggctggcc agaaccagca caaggatggc agaactgagg acactcttca
     4681 tgacggaaat ataggaacaa agcaccagaa aacgtcgcca atccgggccg cccccggaaa
     4741 aatatcgcaa ccacggacac ttttctgtct atacttc
//
LOCUS       DAOSHE010000120         4761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16016_length_4761_cov_4.059499, whole genome shotgun sequence.
ACCESSION   DAOSHE010000120 DAOSHE010000000
VERSION     DAOSHE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16016_length_4761_cov_4.059499"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLD82_04560"
     CDS             complement(<1..243)
                     /locus_tag="PLD82_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG64692.1"
                     /translation="MRIQLVTLFPEIVTPWIQSGILGRAVSKGLVSVSAVNPRDWARD
                     IHHRVDDYVYGGGPGMLLKPDVMQECITALRKKGDRV"
     gene            complement(260..757)
                     /gene="rimM"
                     /locus_tag="PLD82_04565"
     CDS             complement(260..757)
                     /gene="rimM"
                     /locus_tag="PLD82_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG64693.1"
                     /translation="MIVVAKVGGAHGLSGEVRVRFFSDDPEECCARGPLYPADDQGVS
                     PWRVRTLRQGNTMAIVQFEGIRNRAAAEKLNGTLLYQPRECFPDPLDDEYYYADLVGM
                     RVIRADDQQEIGAVTEVFSSGASDILVVRGVDGKCINIPFINQAVPVVDKDTRTIAIL
                     PEFIP"
     gene            complement(761..1018)
                     /locus_tag="PLD82_04570"
     CDS             complement(761..1018)
                     /locus_tag="PLD82_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG64694.1"
                     /translation="MQEKELVEYIVKSLVDSPEDVSINVIKGEKSTIMELKVLPRDVG
                     KVIGKHGRIAQALRTIITAAAMKAGKRIVLEILDNPRDENE"
     gene            complement(1087..1320)
                     /gene="rpsP"
                     /locus_tag="PLD82_04575"
     CDS             complement(1087..1320)
                     /gene="rpsP"
                     /locus_tag="PLD82_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG64695.1"
                     /translation="MRLMRGGTKRKPFYKIVVADINSPRDGKFIEMVGRYHPLNKLEP
                     VVLNDERIKYWLSNGVQPTKIVHDILKKKGVQA"
     gene            complement(1350..2687)
                     /gene="ffh"
                     /locus_tag="PLD82_04580"
     CDS             complement(1350..2687)
                     /gene="ffh"
                     /locus_tag="PLD82_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG64696.1"
                     /translation="MFEQLTARISGILGSFGNKKLTEANVREGMREVRLALLEADVAV
                     RVVDEFVESATTKALGEEILRSVSPTQQFIKVIYDELVRVMGGAGQQFALRKGKLNIL
                     LCGLQGSGKTTTAAKIALMHKKQYRFLLVSADTYRPAAMEQLKILAAQAGVGFFEQGM
                     KLKNPVDIVRKARSYADENGYNGLIIDTAGRMEVDEAMMKEIQQISKLVKFDETFFVA
                     DATAGQSIVQTVSAFHEQLHLTGLVLTKFDSDTRGGAALSVKAVTGKPIAWVGTGEKL
                     EDFEIFYPDRIASRILGKGDILTLIENAQKVIEAEDAKKLEEKFRANDFNLEDFRDQI
                     ARLRKMGPLSKVMGMLPGVDAEKQKDFNERDLIRIEAIINSMTKFERRNVNILGPTRR
                     KRIAKGSGTTLAEVNRVLTRFQDMKRMMKKLSKNPQMFDRFSEADMQGIPPLS"
     gene            complement(2779..3843)
                     /locus_tag="PLD82_04585"
     CDS             complement(2779..3843)
                     /locus_tag="PLD82_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64697.1"
                     /translation="MRSCLLSAIILATGCIGLLALESFEQKTIEVRVGDLVTLPLQPL
                     AGGSWEHRLITPALLTILSNRRLDDRMELLVKALAEGTGRIETVRIVSNRVVLRKYFF
                     FRIAKRSSVAVTNAQTDLTNGTDMPRDGKESAEDRDFSQALRVYEEGEYDEALRAFEL
                     FVRRYPRSSRLGLVKLYAGQSRYALGQYQQALADFRIAGSSEDERVRLLASMWTGHAA
                     EASGNLDEAVEAFMNAMSPQHPDIDIRARTGLAVTYGRRGRLALAGAQFTRIFRLYAA
                     TRRQNPGYLPALYHAARFYDRDARDAESAVRLYREFLELAQDSLRSSSSPVGMPELSR
                     QTAEVRARLAWLRANYTDYR"
     gene            complement(3840..4268)
                     /locus_tag="PLD82_04590"
     CDS             complement(3840..4268)
                     /locus_tag="PLD82_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64698.1"
                     /translation="MKQDFDLLEELKRNKWPLAGMSVAVFILTLLLFSKGSDPPSSRQ
                     VEEKEFPTEESRPIEKKDGVLLDLSEQLFEADRFSLAGPVRFFIRGAGTNLVQFQRKM
                     YELDESILDPRAFPGTEFVRYHKETRTNPKMPGRTNGGKR"
     gene            complement(4309..>4761)
                     /locus_tag="PLD82_04595"
     CDS             complement(4309..>4761)
                     /locus_tag="PLD82_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOG64699.1"
                     /translation="VNFHKIFRRLFDGGKAELNLTNPENVLESGIEILVQPPSKALQN
                     EALLSGGEASMTAIALLFAIFMVKPSPFCLLDEIDAALDGPNVERFKKMLLEFRETTQ
                     FLIISHNINTLKVADALYGISMEEDGVSTALSLDVNELEKLKKKYEVT"
BASE COUNT         1062 a         1365 c         1219 g         1115 t
ORIGIN      
        1 cacccgatca ccctttttcc ggagagccgt gatgcactcc tgcatgacat ccggtttgag
       61 caacatcccc ggaccgccac cgtacacgta atcatcgacc ctgtgatgga tgtcgcgtgc
      121 ccagtcacgg gggttgaccg cactgacgga tacaagtcct ttagaaaccg cccgccccag
      181 aatcccggat tgtatccaag gggtgacgat ctcgggaaag agtgtgacaa gctggatgcg
      241 catggcttct ccggaaagat caggggataa attcgggaag gattgcgatc gtccgggtat
      301 ccttgtcgac aacagggaca gcctggttga tgaatgggat gttgatgcac tttccatcaa
      361 ctccacgaac cacgaggata tcactggcac cactggaaaa aacctcggtc acggcaccaa
      421 tctcctgttg atcatctgcg cggatcacac gcataccgac cagatcggca tagtaatact
      481 catcatcgag ggggtcggga aaacactccc tcggctgata gagcaaggtt ccattgagtt
      541 tttcagcggc agccctgttc cgtattccct cgaactgcac aatcgccatc gtgttgccct
      601 gacggagcgt ccgcacacgc caaggagaga cgccctgatc atccgccgga tagagcgggc
      661 ctcgggcgca acactcctcg ggatcatcgg aaaaaaaccg gacgcgcacc tctccggaga
      721 gtccgtgcgc gccaccaact tttgcaacaa caatcatgaa ttattcgttc tcgtcccggg
      781 gattgtccag aatctcgagg acaatccttt tcccggcttt catcgccgca gcggtgatga
      841 tcgtccgcaa ggcctgagca atccggccat gttttccgat aaccttaccc acatcacgag
      901 gcaaaacctt gagctccatg atggtggatt tttcgccctt aatcacattg atcgacacat
      961 cttcaggact gtccacaagc gacttgacaa tgtactctac cagttccttt tcctgcatac
     1021 cgggctctcc tttcaagggc ttagtgtgct gcactgagac gggcgcgccg gattgaaaaa
     1081 gaacactcag gcctgaacgc cctttttctt gagaatatca tgcacaatct tggtcggctg
     1141 gacaccgttg gagagccagt acttgattct ctcgtcattg agcacaacag gctccagctt
     1201 gttgagcggg tgataccggc caaccatttc gataaacttg ccgtctctgg gcgagttgat
     1261 atcggctacc acaattttgt aaaaaggctt gcgctttgta ccaccacgca tcaatctcat
     1321 gcgaaccaag ggtgtctcct caaattaatc tatgacaggg gcggtatccc ctgcatgtct
     1381 gcctcactaa atctatcaaa catctgcgga tttttcgaca actttttcat catccgcttc
     1441 atgtcctgaa acctcgtcaa aaccctgttg acttcggcca gagtggtccc gcttcctttg
     1501 gcgatccgct tccgtcgcgt tggtccgaga atgttgacgt tccgtctttc gaactttgtc
     1561 atcgaattga tgatggcttc gatccggatc aaatcccgtt cgttgaagtc cttctgcttc
     1621 tcggcatcta cgccgggaag catgcccatg accttggaga gtggtcccat cttgcgcaat
     1681 cgcgctatct ggtcgcgaaa atcctcaaga ttgaaatcat tggcccggaa tttctcttcc
     1741 agctttttgg catcctcggc ctcgatcact ttctgcgcat tttcgatcag ggtcaggatg
     1801 tctcccttgc ccaaaatccg ggatgcaatc ctgtccggat agaatatctc gaaatcctcg
     1861 agtttttcac cggttccgac ccaggcgatc ggtttcccgg tgaccgcctt gaccgagagg
     1921 gcggcaccac cccgggtatc actgtcgaac tttgtcagga ccagtcccgt gaggtgcaac
     1981 tgttcgtgaa aggcggaaac ggtctggaca atggattgac cggctgtcgc atcggccaca
     2041 aagaaggtct cgtcgaattt gacgagtttg gagatctgct ggatctcttt catcatcgct
     2101 tcatcgactt ccatccggcc ggccgtatcg atgatcagcc cattataccc gttctcgtct
     2161 gcatacgatc gtgctttgcg gacaatatca acaggattct tgagcttcat tccctgttca
     2221 aaaaatccta cacccgcctg tgcggcaaga atcttgagct gctccatggc ggccggacga
     2281 taggtatcgg cagagaccag taaaaaccgg tactgctttt tatgcatgag tgcgatcttg
     2341 gcggctgttg ttgtctttcc ggaaccctgg agtccgcaga ggaggatgtt cagctttccc
     2401 ttcctgaggg caaactgctg ccctgcgcca cccatgactc gaaccagctc gtcgtagatc
     2461 accttgatga actgctgcgt cggactgacg gaacggagaa tctcctctcc gagcgccttg
     2521 gttgtcgcac tctcgacaaa ttcgtcaaca accctgaccg cgacatcagc ctccaagagg
     2581 gccaagcgga cctcccgcat gccttccctg acattcgctt ccgtcagttt tttgttcccg
     2641 aaacttccaa gtatcccgct gatgcgggcg gtcaattgct caaacatggc gtgggtctct
     2701 tcccgaacaa gaaatgcact gcaaccataa aggtatcaag atggcggttt cccggcaacc
     2761 ggtgagaaca agggcgtatc accggtaatc ggtataattt gcccgcaacc atgccaaacg
     2821 ggcccgcacc tcagccgttt gcctcgagag ttcgggcata ccgacaggac tggaggagct
     2881 tcgcagggaa tcctgggcca attcgagaaa ctctcgatac agccggactg cggattcagc
     2941 atcccgggca tcccggtcat aaaatctggc cgcgtgatag agggccggga gatagcccgg
     3001 attctggcgt ctggttgcgg catacaatcg gaagatccgg gtgaactggg caccggcaag
     3061 agccagcctt ccccgccgtc cataggtcac tgccaggcct gtcctggccc gaatatcgat
     3121 atcagggtgc tggggactca tggcgttcat gaaggcttcg acagcctcat ccagattgcc
     3181 cgaggcttcg gccgcatgtc cggtccacat actggccaga aggcggaccc gttcatcctc
     3241 actggacccg gcgatcctga aatctgccaa tgcctgctgg tattgtccaa gggcatagcg
     3301 agactgtccg gcatagagct ttaccagccc cagtcttgaa ctgcggggat atcggcggac
     3361 aaagagttca aaggcccgga gggcctcatc atactcccct tcctcataca cacgtagtgc
     3421 ctgtgaaaaa tccctgtctt cggcactctc cttcccgtca cggggcatgt cagtcccgtt
     3481 tgtcaaatcg gtctgcgcat tggtcaccgc gacggaggac cgttttgcaa tcctgaaaaa
     3541 aaagtacttc cgcaagacga cccgatttga tacgatccgc acggtttcaa tcctgcccgt
     3601 tccctcagcc aaggccttga ccaggagttc catccggtca tccaggcgcc tgttgctgag
     3661 gatcgtaagc agggccgggg taatcagacg atgctcccag gaaccacccg ccaatggctg
     3721 gagcgggaga gtgaccagat caccgacccg gacctcgatg gtcttctgct caaaactctc
     3781 aagggccaaa aggccgatac aacctgttgc gagaataatt gcagagagca agcaggatct
     3841 catcttttcc ctccattggt tcggccaggc atcttcggat tggtcctggt ctctttgtga
     3901 taccggacaa actcggtccc gggaaaggcc cgcgggtcga gaatggattc atcaagttcg
     3961 tacatcttgc gctgaaactg gacgagattt gttcctgctc cacggatgaa aaaccggacc
     4021 ggaccagcca gggagaatct gtccgcctca aaaagctgct cagagagatc caggaggact
     4081 ccgtcttttt tctcgatcgg tcgagattcc tctgtcggga attccttctc ttcaacctga
     4141 cgcgacgacg gcgggtcgct ccctttggaa aagagcagga gtgtcagaat gaagacggcg
     4201 acactcatcc cggcgagcgg ccatttgtta cgcttgagtt cttcgaggag atcgaaatcc
     4261 tgtttcatga tagcggatga caccccatct ctctgcccgg caacggaatc aggttacctc
     4321 atacttcttt ttcaactttt caagttcatt gacatccagt gacaatgcgg ttgaaacgcc
     4381 gtcctcttcc atcgagatgc cgtagagtgc gtccgcgacc ttgagggtat tgatgttatg
     4441 cgaaatgatg aggaattgtg tggtctcccg gaattcaaga agcatctttt tgaatcgctc
     4501 gacattcggg ccgtccaagg cagcgtcgat ctcgtccaga aggcagaacg gactgggctt
     4561 gaccatgaag atggcgaaca acagggcgat ggcagtcatt gaagcctctc caccagagag
     4621 aagtgcttcg ttctgcaagg cttttgatgg cggttggaca aggatctcga tcccggactc
     4681 gagaacattt tcgggattcg tcaggttgag ttcggccttg cccccgtcaa acagccgccg
     4741 gaagatcttg tggaaattga c
//
LOCUS       DAOSHE010000121         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16021_length_4760_cov_3.836132, whole genome shotgun sequence.
ACCESSION   DAOSHE010000121 DAOSHE010000000
VERSION     DAOSHE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16021_length_4760_cov_3.836132"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="hydG"
                     /locus_tag="PLD82_04600"
     CDS             <1..1000
                     /gene="hydG"
                     /locus_tag="PLD82_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOG64700.1"
                     /translation="YGEHPQSDYDYIAQTLKVAYSVKSGNGEIRRVNVNAAPLSVDSL
                     RVLRDVGIGTYQVFQETYDRKTYANIHPSGLKANYRWRLYALHRSLEAGADDVGMGAL
                     FGVSDWRYEILGLLAHTIDLENRFGGVGPHTISFPRIEPAVNTPWIDNSAWKIDDETF
                     IRIVAIIRLMVPYTGMILTCREPAAVRERLLDQGVTQLDFGSNIGVGAYSQAREDHAR
                     QQFVLSDTRTLDEGIRWLASRGRITSFCTADYRLGRTGRQFMCIAKKGHIQHMCRPNA
                     VLTFMEYLLDYASPETRSVGAALVERELDGITPPELQRHTRELLARIWNGERDLYI"
     gene            1518..1862
                     /locus_tag="PLD82_04605"
     CDS             1518..1862
                     /locus_tag="PLD82_04605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG64701.1"
                     /translation="MTASTDKRRHPRFELTLPVKYQLFGADGGDHGCGISRSLDVSKG
                     GLKLVGSANAVPGDGISITVHSGADGITLKGRVCWVHQLAYRQCEIGVSFDEIQADEE
                     ERLYRLLEGLST"
     gene            1900..2850
                     /gene="tal"
                     /locus_tag="PLD82_04610"
     CDS             1900..2850
                     /gene="tal"
                     /locus_tag="PLD82_04610"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOG64702.1"
                     /translation="MSLLEQLKAMTKVVADTGDFQSMKQYKPVDATTNPSLIYAAAGD
                     PQYAHLVDEAILWAGKQSGDPAKRLALCLDKLAVNFGIEILKIVPNRVSTEVDARLSF
                     DTGATIAKARDLIALYKEAGIPRERILIKVAATWEGIEAARVLEKEGIHTNLTLLFSL
                     VQAVACAEAGVTLISPFVGRILDWYKKDRGVDAIPAIEDPGVLSVATIYNYYKHHGHK
                     TEVMGASFRNTGEIVELAGCDLLTIAPSLLKELESMDGSLVRKLDPATISPVDRIAVE
                     EKAFRWLLNEDAMATEKLSEGIRKFAADLVKLENSIRARV"
     gene            complement(2906..4099)
                     /locus_tag="PLD82_04615"
     CDS             complement(2906..4099)
                     /locus_tag="PLD82_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG64703.1"
                     /translation="MSKTAIESIVETRDKRAEQTLGQYACHFSEAKRLRTDSGRADDA
                     SIRTPFARDSDRIIHSRAYARYIDKTQVFSFFENDHITHRVLHVQFVSRIGRTIGRSL
                     ALNEDLIEAISLGHDIGHAPFGHFGEKVINTICHDEKIGCFYHNVQGVRFFHEIERNG
                     TGINLTVQTLDGIICHNGEVLQSSYRPDRSKTPEEMLAEYRRSLVEDGVGINLVPMTL
                     EACVVRIADVIAYIGRDIEDAIHIGLIKRSDLPPSVTGILGSDNAAIIDHLVLDLLEQ
                     SYGRDEIRFSEPVFTALKELLSFNYSYIYTNRRIMREHGKVARIIRMLYDKYHAEMQE
                     GFVENCPFAHHCRELGPDFVKRQGPRIIIDFLAGMTDDYLINQFRSIFMPDSYGYKLN
                     EDRAR"
     gene            complement(4469..>4760)
                     /locus_tag="PLD82_04620"
     CDS             complement(4469..>4760)
                     /locus_tag="PLD82_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64704.1"
                     /translation="TVAILPLNDETLAAEETTGTEENADDTTEDCDETLAAEETTGTE
                     EEEIGPEEFADDAELPTEDCATDEETGTPEADESDSPDWADDDKAVEPEATD"
BASE COUNT          942 a         1251 c         1459 g         1108 t
ORIGIN      
        1 ctacggtgag catccgcagt cggattatga ctatattgcg caaaccctca aggttgccta
       61 cagcgtcaag tccgggaacg gagagatccg ccgtgtcaat gtgaatgccg cgccgctctc
      121 ggtcgattca ctgcgggttc tgcgtgatgt cgggattggg acgtatcagg tgttccagga
      181 gacctatgac cggaagacct atgccaacat ccacccgtcc ggtctgaagg ccaattatcg
      241 ctggagactc tatgctttac accgttccct cgaggccggg gctgatgatg tggggatggg
      301 tgccctcttt ggtgtttccg attggcgata tgagattctt ggccttttgg cacatacgat
      361 cgatctcgaa aacaggttcg ggggtgtcgg gccgcacacg atttctttcc ctcggataga
      421 accggccgtc aataccccat ggatcgacaa ctcggcgtgg aagatcgatg atgaaacctt
      481 tatccggatc gtggcgatca ttcgcctgat ggttccctat accgggatga tcctgacctg
      541 tcgcgagccg gccgcagtcc gtgaacggct cctggatcag ggtgttaccc agcttgattt
      601 cggttcgaat atcggggtgg gtgcctacag tcaggcccgt gaagaccatg ctcggcaaca
      661 gtttgtcctg tccgatacac ggacgctgga tgagggaatc cgctggctgg cctcacgcgg
      721 gcggattacg tcgttttgca cggccgacta tcgtttgggg cgcaccggcc gccagttcat
      781 gtgtatcgcc aagaagggac atatccagca tatgtgccgg ccgaatgcgg tgcttacctt
      841 catggaatac cttctcgatt acgcatcacc ggaaacacgt tcggtcgggg cagcgcttgt
      901 ggaacgggaa ctggacggga tcaccccccc ggaactgcag cgccataccc gcgagcttct
      961 cgccaggatc tggaatggtg aacgggatct ctacatatga tgaatgatgg ggaacagcct
     1021 caggccattc ccggatcggc gcgaacgggt gacggaaggg tgattaccga cctggatctg
     1081 gccagggtcg cggagcggta cctgcaagac catcctcagg cgtcttctgg attacccgga
     1141 ggttcgggcg tcgagagtcc tgggtcgccg gaagcccgaa tgcggcgggt ctcccgcttc
     1201 cgtcccggga gcgggcggag tgttgccggg tgtacatggt gcataccccg gatggcggga
     1261 tccccgccgg tgccgtccgc gatgtgttat cggccatgct ggaatcccgg gagtgagagt
     1321 gggcgtccgt tctgcacggc tcggtagatg cttccggagt cgccctgcaa acccgcaact
     1381 gagcagccga aggacctgta gggggcgtgt cgccggtctt cttttccgcc cggggtgcgt
     1441 cagggatctt gcgttccaat atccgattgg gctatactgc actccagcac catggaccag
     1501 cccggggagg agaacggatg acagccagta cggacaagcg acggcatccc cggtttgaac
     1561 tcaccctgcc ggtcaaatat caactctttg gagccgatgg tggcgatcat ggatgcggga
     1621 tttcgcgctc ccttgatgtc agcaaggggg gattgaaact cgtcgggagt gccaatgccg
     1681 ttcccgggga cgggatatcg atcacggtcc attccggggc ggacgggatc accctcaagg
     1741 gcagagtgtg ttgggtccat cagttggcat atcgccagtg tgaaatcggg gtgagcttcg
     1801 acgagattca agccgacgag gaagagcgtt tgtaccgtct tctggagggg ctttccacgt
     1861 gaaacgcgta tggacgcgaa catatcatgg aggttgcgaa tgagtctctt ggaacagttg
     1921 aaggccatga ccaaggtggt tgcggacacc ggagattttc agtccatgaa gcagtacaag
     1981 ccggtcgatg cgacaaccaa cccgtccctg atttacgcgg cagcgggtga tccacagtat
     2041 gcccatcttg tcgatgaagc gattctctgg gccgggaagc agagcgggga tcccgccaag
     2101 cggctcgcac tctgtctgga taaactggcg gtcaatttcg ggatcgagat cctcaagatt
     2161 gtccccaacc gtgtctcgac cgaagtcgat gcacggctct cgtttgatac cggggcaacc
     2221 atcgccaagg cccgggacct gattgccctg tacaaggaag ccgggattcc gcgcgagcgg
     2281 attctgatca aggtcgccgc aacctgggag ggtatcgagg ctgccagggt tctcgaaaag
     2341 gaagggatcc ataccaatct cacactcctg ttcagtctgg tgcaggcggt tgcctgcgcc
     2401 gaggccgggg tcaccctgat ctctccgttt gtcggacgga tcctcgattg gtacaagaag
     2461 gatcgcgggg tggatgccat tccggctatc gaagacccgg gagtgctttc cgttgcgacg
     2521 atctacaatt attacaaaca ccatgggcac aagaccgagg tgatgggggc gtccttccgg
     2581 aatacgggcg agattgtcga actggcaggt tgtgaccttt tgacgattgc gccgtcactg
     2641 ctcaaggagc ttgaatcgat ggatggcagt ctggtccgga agctcgatcc tgcaacaatt
     2701 tcaccggttg acaggatcgc tgtcgaagaa aaagcgttcc gctggctcct gaacgaggat
     2761 gcgatggcaa ccgaaaaact ctccgaaggt atccggaaat ttgcggccga tcttgtcaag
     2821 ctcgaaaact cgatccgcgc cagagtgtaa gccgacgggg caaggggttt ggccgctctc
     2881 tgcctgaaca accgggcctg ccttttcagc gggcccggtc ttcattgagt ttgtagccgt
     2941 aactgtcggg cataaagatg ctgcggaact ggttgatcag gtaatcgtcc gtcatgccgg
     3001 ccagaaaatc gatgatgatg cgcggaccct gccgcttcac aaaatccggt ccgagttcgc
     3061 ggcaatgatg ggcaaagggg cagttctcga caaagccctc ctgcatttca gcatgatatt
     3121 tgtcatagag catgcggatg atccgggcga ccttgccgtg ttcccgcatg atccggcgat
     3181 tggtgtagat gtaggagtaa ttgaaggaga gaagttcctt gagggccgtg aagacaggtt
     3241 cgctgaagcg gatttcgtca cggccatagc tctgctccaa aaggtcgaga accaggtggt
     3301 cgatgatggc tgcattgtcg gagccgagga ttccagtgac cgaaggcggg aggtcgctgc
     3361 gcttgatcag tccgatatga atcgcgtcct cgatatcgcg cccgatatag gcaatcacgt
     3421 cggcaatgcg gacgacacag gcctcgaggg tcatcgggac gagattgatc ccgacaccat
     3481 cttcaacgag gctgcgccgg tattcggcga gcatctcttc cggggtcttg cttctgtcgg
     3541 gacggtagct gctttggagg acctccccat tgtggcagat gatcccgtcc agtgtctgga
     3601 ccgtcagatt gatgccggtc ccgttccgct cgatttcatg aaagaaacgg acaccctgga
     3661 cgttgtgata aaagcatccg attttctcgt cgtgacagat cgtgttgatc actttttcac
     3721 cgaaatggcc aaagggcgca tggccgatgt cgtggccgag ggagatggct tcgatcaggt
     3781 cttcgttgag ggccagggag cgcccgatgg tccgcccgat ccgcgagacg aactggacat
     3841 gcaggactct atgcgtgatg tggtcgtttt caaaaaatga aaagacctgg gtcttgtcta
     3901 tataccgggc ataggcccgg gagtgaatga tgcggtccga gtcacgggca aaaggtgtgc
     3961 ggatgctggc atcgtcggcc cggccggagt cggtgcgcag gcgttttgct tccgaaaagt
     4021 ggcaggcata ttgtccaagg gtctgttcgg cgcgtttgtc cctggtttcg acgatggatt
     4081 cgatggctgt ttttgacatg ggtccctcct gcatccagtg tccattgtac cggacacact
     4141 gtggtgactg caaggggcga aacgcgcgtg ctgtgtccgg gctattctcc ctgcttgttc
     4201 aatttgggcg gcgaccctgt cattagaaga cagacttgca caaaaaacct ggagcaattt
     4261 acattatata taaaaattat catgttgtga atatgttatt gcgctatact tcaataatga
     4321 aacatgtaac ggagcgcctt atggtaaaaa cctttgtttt gtgtgttgtg ttgagtctgt
     4381 ttgtcgtttt ttcggcttgt tcggatgatg cggttgtgac tccgttggat gggtcgagca
     4441 gcagtacggt ttcctggtct gtctcaagtc agtccgtagc ttcgggttcg actgctttgt
     4501 catcatcagc ccagtcaggg ctgtcgcttt catcggcctc cggagtcccg gtttcctcgt
     4561 cagtggcgca gtcttcagtg ggaagctcgg cgtcatccgc gaattcttcc gggccgatct
     4621 cttcctcttc tgtcccggtt gtctcctcag cggccagtgt ttcgtcacag tcttctgtcg
     4681 tgtcgtcggc gttttcctcg gttcctgttg tctcctccgc ggccagcgtt tcgtcgttca
     4741 gtggcaatat tgccacggtt
//
LOCUS       DAOSHE010000122         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16349_length_4688_cov_4.877833, whole genome shotgun sequence.
ACCESSION   DAOSHE010000122 DAOSHE010000000
VERSION     DAOSHE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16349_length_4688_cov_4.877833"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLD82_04625"
     CDS             complement(<1..772)
                     /locus_tag="PLD82_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG64705.1"
                     /translation="MSFKRQTGLWHAFRFFQDLALVYPVYVILLLQHGLGAGSVALIL
                     ALWAGSMLVFELPSGILADRWSRRKTLIAGMLAKAIGFACWLVWTTPAGFAVGFVFWG
                     LQEAFVSGTSDAYLFGLLRRSDRADRFETVSARAAIWSRLAIILSMLGGAWLAARDSA
                     LCIALSALAMLLSGLCLWLLPEIDRNRQPGRHEDETCAGPTIRELATSLRSSLRLPGM
                     LTSLVFGCGVLAGWGMLDEYDTAYGMHRGLPLELVGLWG"
     gene            complement(769..1269)
                     /locus_tag="PLD82_04630"
     CDS             complement(769..1269)
                     /locus_tag="PLD82_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64706.1"
                     /translation="MELFARCTIADHGIACFWGTNFIEVTRGEQPKKVLIPGESVEAL
                     AAAAGPDILWLALTGDTTRPCVWYDPAQDAWHELDLADHRRRFGSMTVLPCILTGETG
                     LPVWWLATGYEAPGTTLVIGLSDPLSIPEVFPLPGLFISARMVDGQPLVRTSAGEQRL
                     AGGITR"
     gene            1460..1618
                     /locus_tag="PLD82_04635"
     CDS             1460..1618
                     /locus_tag="PLD82_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64707.1"
                     /translation="MLTRLLLALLALAVAVFGFTVAGCRGAMYGPPEGYTNTEPSQVS
                     SDTNTQKL"
     gene            1620..2741
                     /locus_tag="PLD82_04640"
     CDS             1620..2741
                     /locus_tag="PLD82_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOG64708.1"
                     /translation="MSGKETPTGGGIPWRKRMAMSAFARWRHAQARLHPLTYLFWECT
                     LRCNLACRHCGSDCHVSSQQPDMPLEDFLAVLDRVATISDPAKTLIAITGGEPLVRDD
                     LAACGRAISERGFPWGMVTNGWALDQKQLDGLLASGLRALTVSLDGLEDSHDWLRGRA
                     GSYRRALAAIRCVSTVPQIAFDIVTCVTPRNIGELPALREVLIDAGVPAWRIFNVFAK
                     GRAATDPELVTDGGLLKKTLDYVCQARGSGGLRVNYACEGYLGPYEYEARDGFYNCRA
                     GTNIASVLADGSISACPSLRGDFIQGNMYRDDFVDVWNTRYQNMRERSWLRTGICREC
                     SSWKWCEGNALHLRDDAGHLARCHLALLEEAEGTGTSCR"
     gene            2810..>4688
                     /locus_tag="PLD82_04645"
     CDS             2810..>4688
                     /locus_tag="PLD82_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG64709.1"
                     /translation="MKVFSCAFGILVALLVPAISRGITLDTVWKATDRYWKVWEKDSD
                     HAHARAILPILEQACREYPDKADFFSTRIFVLTQLKRDDEAIRLGGDSYTRFPKHRWV
                     REHYKNALTSAGWRAVDAGLKDKAAVWFALAWRHWPDDPDVVNAWGFSLYDHHNRPAD
                     AIAILEPALRRHPDHRWLPQTLAWSWYKHGDQLFAAGQTNAASAAWDNFWKTIGPDNQ
                     HAHLNYLWKLHALGRYQDAQPVIADALKRTGMTDELYRCIYWLTSGQIDRLRKARPAM
                     QGREDVFTMYKKLYLHAASRDREWKNGITYRHLTVMKAYHELNQDHDSICPYWRTYAP
                     KERVAAQNILAALERILPPDFEIVVRNFRAQVLYRQERFAETAREMTALSLAAFRLPW
                     AGTQGYAQPLVLPFPVRGICRVTSRHSQRYITHMGLNRECYDIWGTDTAGRWVADGAD
                     RTRNESWYGFGREIIAVVDGVVETAVGQHPDDPPESTVLGPSGNYVVIADSAGRKYNY
                     YHLKQHSVRVRVGQKVRRGEVIALLGNSSSTSAHLHFGVYSPDWLVSLPVFFERYHVI
                     KDGIRSEIVRGQPAGNGSRKTKTRLIPEILHISRLNTLISRLTDDCPCKGMGREVFKT
                     GC"
BASE COUNT          938 a         1379 c         1439 g          932 t
ORIGIN      
        1 agccccagag accgaccagt tcaagcggga gtccccgatg cattccgtag gctgtatcgt
       61 actcgtcgag cattccccag ccggccagca ccccgcagcc gaagacgagc gaggtgagca
      121 tgccggggag ccggagggac gagcgcaggc tggtcgcaag ttcccgaatg gtcggtccgg
      181 cgcaggtctc atcctcatgt ctgcccggct ggcgattcct gtctatttcg ggcaggagcc
      241 agagacagag tcccgagagg agcatggcca gggccgagag cgcgatgcag agcgcgctgt
      301 cccgtgcggc cagccaggcc ccgcccagca tcgagaggat gatggccagc cgtgaccaaa
      361 tcgcagcccg ggccgagacg gtttcaaacc ggtcagccct gtctgaccgc ctgaggagtc
      421 cgaagaggta ggcatcggac gtgcccgaga cgaaggcctc ctgcaggccc cagaaaacaa
      481 aaccgacagc aaaaccggcg ggggttgtcc agacaagcca gcaggcaaag ccgatggcct
      541 tggccagcat gcccgcaatc agggtcttgc gccggctcca gcggtcggcg aggattccgg
      601 acgggagttc aaaaacgagc atgcttccgg cccagagggc gaggatcagg gccacgctcc
      661 ccgcaccgag cccgtgctgc aggaggagga tcacgtagac cgggtagacg agggccagat
      721 cctgaaaaaa acggaaggca tgccagagtc cggtctgtcg cttaaagctc accgggtgat
      781 tccaccagcc agacgctgtt caccggcaga cgtacggacg aggggctgac cgtcaaccat
      841 ccgggcagag atgaagagcc cgggcagggg gaagacttcc gggatcgaga gcggatcgga
      901 gagtccgatg acgagcgtcg tcccgggggc ttcgtagccc gtggccagcc accagacggg
      961 aagtcctgtc tctcccgtca ggatgcaggg gaggaccgtc atgctgccga accgcctgcg
     1021 atggtccgcg aggtccagtt cgtgccaggc atcctgcgcc gggtcgtacc agacacaggg
     1081 ccgtgtggtg tcgcccgtca gggccagcca gaggatgtcc ggtcccgctg cggccgcgag
     1141 ggcctcgacc gactcaccgg gaatgagcac ttttttgggc tgttctccgc gggtcacttc
     1201 gatgaagttt gttccccaaa agcaggcaat cccatggtct gcgatggtac accgtgcaaa
     1261 aagctccatc tcgtatctcc cggtttgaaa tgatgaagag atgatacttc cgggatttcc
     1321 gggagacaaa cggcaaacac accgtatcga ccccgagcgc tctgtacgga aaattcattc
     1381 aagatagacg ggaatgtggt atactgttgg gcaggcgggc gctgctcccg ctgcaatatc
     1441 catcatgaag gagcccgcca tgttgacccg cctgcttctt gccctcctgg cactcgccgt
     1501 cgccgtcttc gggtttaccg ttgcggggtg ccgcggagcc atgtacggac cacccgaggg
     1561 gtataccaac acggagccgt cccaagtgtc gtccgacacg aatacgcaaa agttgtaaga
     1621 tgtcgggaaa agagacgccg accgggggcg ggataccctg gcgcaaacgc atggccatgt
     1681 cggcctttgc ccgctggcga catgcccagg cccgcctcca tcccctgacc tatctgtttt
     1741 gggaatgtac cctgcgctgc aatctggcct gtcgtcattg cgggagtgac tgccatgtct
     1801 cgtcgcagca acccgacatg ccgcttgagg atttcctcgc tgtgctggac cgggttgcca
     1861 ccatctccga tcctgccaaa accctgattg ctattaccgg gggcgaaccc cttgtgcgtg
     1921 acgaccttgc ggcctgcggc cgggcaatca gtgagcgcgg atttccctgg gggatggtca
     1981 ccaacggctg ggctctcgac cagaaacagc ttgacgggct gcttgccagc ggcctgcgtg
     2041 cgctgaccgt cagcctcgac gggcttgagg acagtcatga ctggttgcgc ggtcgggccg
     2101 ggtcataccg gcgggccctt gcggccattc ggtgcgtgag tactgttccc caaatcgctt
     2161 ttgacattgt aacctgcgtc actcctcgca atatcggaga gctgcctgcc ctgcgggagg
     2221 tcctcatcga cgccggtgtt ccggcctggc ggatcttcaa tgtttttgcg aagggcaggg
     2281 ctgcgactga tcccgaactt gtgactgatg gcgggctcct caaaaagacg ctggactatg
     2341 tttgccaggc ccgtggcagc gggggcctcc gggtcaacta cgcctgcgag gggtatctcg
     2401 gaccgtacga gtacgaggcg cgcgatggct tttacaactg ccgtgccggg acaaatatcg
     2461 cttcggtcct ggctgatggc tcgatctcgg cctgcccctc gttgcggggg gattttatcc
     2521 agggaaatat gtatcgtgac gattttgtgg atgtctggaa tacacgatat cagaatatgc
     2581 gggaacgttc ctggctcagg accgggatct gtcgcgagtg ttcaagctgg aagtggtgtg
     2641 agggtaatgc cctccatctc cgtgatgatg caggacatct tgcccgttgt catctcgcac
     2701 tccttgaaga ggctgagggt acagggacaa gttgcagata gaacggcttg tggtgcatgc
     2761 tttcctgttt catggggaac agcctcaggc ccgatgatgg gagactgtca tgaaagtgtt
     2821 ttcatgcgct tttggaatcc tcgttgcatt gctcgttccc gccatctccc gggggatcac
     2881 actcgatacg gtctggaagg ccactgatcg ctactggaag gtctgggaga aggacagcga
     2941 tcatgcgcat gccagagcga tcctcccgat tctcgaacag gcctgtcgtg agtatcctga
     3001 caaggcagat tttttttcaa cacggatctt tgtcctgaca cagctcaagc gggacgatga
     3061 agcgattcgt cttggaggag actcgtatac cagattcccg aaacaccggt gggttcgtga
     3121 gcactataaa aacgccctga cctccgctgg ctggcgtgct gtggatgccg gtctcaagga
     3181 caaggccgct gtctggtttg ccctggcatg gcgacactgg cccgacgatc cggatgttgt
     3241 caatgcgtgg ggtttttccc tgtatgatca tcataatcgt cccgccgatg cgattgcgat
     3301 tcttgaaccg gccttgcgcc gtcatcccga tcatcgctgg ctgccccaga ccctggcctg
     3361 gtcctggtac aagcacggag accagctttt tgcggcagga cagaccaacg ccgcatccgc
     3421 cgcctgggac aatttttgga agacgatcgg tccggacaat cagcatgccc atctcaacta
     3481 tctttggaaa ctccacgcac tgggccgtta tcaggatgcg cagcccgtga ttgcggatgc
     3541 cctcaaacgc acgggcatga ccgatgaact ctaccgctgc atctactggc tgacatcagg
     3601 gcagatcgac cggctgcgca aggccagacc agccatgcag ggacgtgagg atgtgttcac
     3661 catgtacaaa aagctctacc tgcatgccgc cagtcgggat cgggagtgga agaacgggat
     3721 aacctatcgt cacctgaccg tcatgaaggc ctatcacgaa ctcaaccagg atcatgattc
     3781 gatctgtccg tactggagaa cctatgctcc gaaagaacgg gtggctgcgc agaacatcct
     3841 tgctgctctt gaacgtatcc ttccccccga ttttgaaatc gttgtccgaa atttcagagc
     3901 gcaggttctc tatcggcagg aacgttttgc agagactgcc cgcgaaatga cggcgctctc
     3961 acttgcggcc ttccgtttgc catgggctgg cacacaggga tatgcacagc ctcttgtgtt
     4021 gccatttccg gttcgcggga tctgccgggt taccagtcgg cacagccagc ggtacatcac
     4081 gcacatgggc ctcaaccggg agtgctacga tatctgggga acggataccg ccggtcgctg
     4141 ggtggccgat ggcgccgacc ggacccggaa tgaatcctgg tacggcttcg gccgagagat
     4201 catcgccgtg gtggacgggg tggtcgagac cgcagtcggg cagcatcccg atgatccgcc
     4261 cgaatcgacc gttcttggtc cgagcggaaa ttacgtggtg atcgctgaca gcgccgggcg
     4321 caagtataat tactatcacc tcaagcagca ctcggtccgg gtccgggtcg ggcagaaggt
     4381 gcgacgaggc gaggtaatcg ccctgctggg aaattcgtcg tccacctcgg cccatctcca
     4441 tttcggtgtc tattcgccgg actggctggt cagcctgcct gtctttttcg agcgctacca
     4501 cgtgatcaaa gacgggatcc gcagcgagat tgtgcgtggc cagcccgccg gcaacggttc
     4561 cagaaagacc aagacccggc tcatacctga aatccttcac atcagcaggc tgaacaccct
     4621 catctcccgc ctgactgatg attgcccctg caaggggatg ggtcgagagg tgttcaagac
     4681 tggttgca
//
LOCUS       DAOSHE010000123         4673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16414_length_4673_cov_3.643352, whole genome shotgun sequence.
ACCESSION   DAOSHE010000123 DAOSHE010000000
VERSION     DAOSHE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16414_length_4673_cov_3.643352"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLD82_04650"
     CDS             <1..311
                     /locus_tag="PLD82_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG64710.1"
                     /translation="LIRLEPGCATGADELGHPGLELGIITGGKARLTVGMETVPLKTG
                     DAVSFASGSPHRLYNCGTGLLTAYWITTPPGSQPGRNIAIPASDREKTTPRSKGGKHG
                     "
     gene            304..1590
                     /locus_tag="PLD82_04655"
     CDS             304..1590
                     /locus_tag="PLD82_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG64711.1"
                     /translation="MGKTTAEKIFEAHLVDMPDNETSVLRIDTVFCHEITTPIAINDL
                     VARGKDRVVDPSRIKAVIDHVTPAKDTLTAQQGKIIRDWARRHAIKDFFDIGRNGVCH
                     ALFPEQGFVRPGATIIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFKAPRTIR
                     FNITGTLRPGVYAKDVILFVIGQIGVNGATNCVMEFAGPVIDAMSMESRMTLCNMAIE
                     AGGTSGICAPDRTTVEYLWPFIKDEFSSKDAALAEYSRWRADEDAVYEKVITYDVSSL
                     EPMATFGYKPDQVKPVRELAGTKVDQVYIGSCTNGRIEDLRIVAEVLRGKKIADSVRV
                     VLSPATPSIYRMALAEGLVDIFMEAGVCLTNPTCGACLGMSNGVLAPEEVAASTTNRN
                     FNGRMGKGGMVHLMSPATAAATALAGVITNSSLYKG"
     gene            1711..2235
                     /locus_tag="PLD82_04660"
     CDS             1711..2235
                     /locus_tag="PLD82_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOG64712.1"
                     /translation="MKTFGGRVLFLDRSDINTDEIIPAKYLTEVSKEALAPYLLEDLK
                     LEGFEQKRDIANRGVVVTRANFGCGSSREHAPWALEVNGIHVVVAENFARIFRQNMYN
                     CGMLAIELPPASISGLFEKFAGKETSCSVSINPAILSLSAGSITAELPFKLEGFDRAL
                     IEAGGWVEYADKKY"
     gene            complement(2376..3341)
                     /locus_tag="PLD82_04665"
     CDS             complement(2376..3341)
                     /locus_tag="PLD82_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64713.1"
                     /translation="MKKNACLFVILFATVFIFANCSIGPDVVGVSDYETRAVTTINGS
                     GSAAYHGLSDRIRIASFNIEIFGTTKAGRANVLWTLAHIATRYDIIAIQEVGSNGNPS
                     DSTATTTMNTYLAAVNGIAGSGAYSYVRGHQYAFVYRNATITKKASGLYSGTKTFAYT
                     PLRANFAVKSNNFDFAMLTIHTSPSLAKTEIPALTTAMSETRTFYSEQDVICCGDFNA
                     DGSYYSAGTSGGDLAGFASSTYVTVIKNGTDTTVSPNNSYTYDRMQLYTASSQDYTGT
                     KGVLKFASYYKVSMLEGTTTTAGKEDALSDHYPIWAEFYKNKDTN"
     gene            complement(3541..4113)
                     /locus_tag="PLD82_04670"
     CDS             complement(3541..4113)
                     /locus_tag="PLD82_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG64714.1"
                     /translation="MRAGMLVVLLGAMLACTGNMTGGKPAVTQGTGEVGRNESSGNVS
                     LTQIEQDFMRILRADPRQKRTRLTLNPVLQRVARERAKDMAQRNYFSHTNPDGDGPNI
                     LLKRAGYRLPSWWGTARNANNIESISGGRSTARAAYDGWMNSPGHKKHVLAEIGFYAG
                     QTVVAIGHYYDARSTYRHYWVFLSAPPEGD"
     gene            complement(4175..>4673)
                     /locus_tag="PLD82_04675"
     CDS             complement(4175..>4673)
                     /locus_tag="PLD82_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOG64715.1"
                     /translation="GIPFRMLEDLVAGRAVYWDITRAMVQTRTGREDVPQTEPEEAAR
                     HLAEIARDHGLVLYETFMTDMLGHKAQVGEARDFLSVLDRFLTRLAEAVVADGGTLVI
                     ISDHGNIEDCATRAHTTNPVPLIVFGPLAGAFAEARSIMDVPGCIWRCIARGAVRQGH
                     PKTDC"
BASE COUNT         1025 a         1379 c         1293 g          976 t
ORIGIN      
        1 ggttgatccg ccttgagccg ggctgcgcca ccggcgccga tgagctggga catcccggtc
       61 tggaattggg catcatcacc ggagggaaag cccggctgac cgtcgggatg gagacggttc
      121 ccttgaaaac cggtgatgct gtctcgtttg cctcgggaag tccgcaccgt ctgtataatt
      181 gcggcactgg tctactgaca gcctactgga ttaccacccc gcccggctca caaccagggc
      241 gaaacattgc catcccggcc agtgaccggg aaaaaacaac accccgttcc aaaggaggaa
      301 aacatgggta aaactacggc agaaaagatt ttcgaggcac acctcgtcga catgccggac
      361 aacgagacat ccgtgctcag gatcgacacg gtcttctgcc acgaaatcac cactccgatt
      421 gccatcaatg acctggtggc ccgtggcaag gacagggtcg ttgacccctc ccggatcaag
      481 gccgtcatcg accacgtcac cccggccaag gacaccctga ccgcccagca gggaaagatc
      541 atccgcgatt gggcgagacg gcatgccatc aaagacttct tcgacatcgg gcgcaacggc
      601 gtctgtcacg ccctcttccc cgaacagggt tttgtccgcc cgggcgcaac gatcatcatg
      661 ggggactccc atacctgcac gcacggggcc tttggcgcct ttgccgccgg tgtcggcaca
      721 accgatcttg aagtcgggat tctcaagggc gtctgtgcct tcaaggcccc ccggaccatc
      781 cgcttcaaca tcaccgggac actccgcccg ggcgtctatg ccaaggacgt gatcctcttc
      841 gtcatcggcc agatcggtgt caacggggcg accaattgtg tgatggagtt tgccggaccg
      901 gtcatcgacg ccatgtcgat ggagtcgcgg atgaccctct gcaacatggc gatcgaagcc
      961 ggcgggacat ccggaatctg cgcaccggac aggacaaccg tcgagtatct ctggccgttc
     1021 atcaaggacg agttttcatc caaagacgcc gctctcgccg aatactcgcg ctggagggcg
     1081 gacgaggatg ccgtctacga aaaggtgatc acgtacgatg tctcgtcact cgaaccgatg
     1141 gccaccttcg gatacaagcc cgatcaggtc aaacccgtcc gcgaacttgc gggcaccaag
     1201 gtcgaccagg tctacatcgg ttcctgcacc aatggccgga tcgaggacct gagaattgtt
     1261 gccgaagtcc tgcgcggaaa aaagatcgcc gactcggtcc gtgtcgttct ttcgccggca
     1321 actccgtcga tctacaggat ggccctggcc gagggactgg tcgacatctt catggaagcc
     1381 ggggtctgtt tgaccaaccc gacctgcggg gcctgtctcg ggatgagcaa tggcgtcctc
     1441 gcaccggaag aagtcgccgc cagcaccaca aaccgcaatt tcaacggacg gatgggcaag
     1501 ggcggcatgg tccatctgat gagtccggcc acggcagcgg cgaccgctct cgcgggtgtg
     1561 attaccaatt caagtctcta caaagggtga acaacctccg gccccttcgc atgcggcgtt
     1621 tcctgcatgt gccttccggg ccaagcgctg ttcacaaaaa ctatgccgtg gccgatcaag
     1681 gggccgcagg cgggaaagga agcatagctc atgaaaacat ttggcggcag ggtcctcttt
     1741 ctggaccgtt ctgacatcaa taccgacgaa atcattcctg caaaatacct gaccgaggta
     1801 tccaaggaag cactcgcccc ctatctcctc gaagacctca agctggaggg ctttgaacag
     1861 aaacgcgata ttgcaaaccg tggcgttgtc gtcacgcggg ccaatttcgg ctgcggcagc
     1921 tcacgcgagc atgctccctg ggcgcttgag gtcaacggca tccacgtggt tgtagccgaa
     1981 aacttcgcgc gcatcttcag gcaaaacatg tacaactgcg gtatgctggc catcgagctt
     2041 cccccggcat ccatttccgg actctttgaa aaatttgccg gcaaagaaac cagttgcagc
     2101 gtttccatca atccggcgat cttgagcttg tcagcaggat caatcactgc ggagcttccg
     2161 ttcaagctgg aggggtttga ccgcgcactg atcgaagccg gtggctgggt tgaatacgct
     2221 gataaaaagt attgaacaca caacatgatg aagcatcgga cattcccctg cggggacttc
     2281 gctacctgca gggaaactga aaaagaagtg ccaccgacca ctcgaaacaa aaagccgcct
     2341 gcgatccggg cggctttttg tcagcacgga cgttttcagt tcgtgtcttt gttcttgtag
     2401 aattcggccc agatcgggta gtggtcggag agggcatctt ccttgccggc cgtcgtggtt
     2461 gttccctcga gcatgctgac cttgtaatag gaggcaaact tgaggacacc cttggtcccg
     2521 gtataatctt gggacgaggc ggtgtagagc tgcatgcgat cataggtata gctgttgttg
     2581 ggcgagaccg tggtatccgt gccatttttg atgactgtca cgtaggtgct cgaggcaaag
     2641 ccggccagat ccccacccga ggttcccgca ctgtaatagg aaccatccgc gttgaaatcg
     2701 ccacagcaga tgacatcctg ctcgctatag aaggtccggg tctcggacat ggctgttgtc
     2761 aaagccggaa tctcggtctt ggccagactc gggctggtat ggattgtcag catggcaaaa
     2821 tcaaagttgt tgctcttgac ggcaaaattg gcccggaggg gagtgtaggc aaaggtcttt
     2881 gttccgctgt acaaaccgga agcctttttc gttatcgtcg cgttgcggta gacaaaggca
     2941 tactggtggc cgcgaacata ggaatacgcc ccggatcccg cgataccatt gactgccgca
     3001 agataggtgt tcatggttgt tgtggccgtt gaatcagaag gattcccgtt ggaaccgact
     3061 tcctggatgg cgatgatatc gtaccgggtg gcaatatggg caagtgtcca gaggacattg
     3121 gcccgaccgg ccttggtcgt gccaaaaatc tcgatgttga aagaagcgat gcggatccgg
     3181 tccgaaagac cgtgatatgc ggcagatccg cttccgttga tggttgtaac ggcacgcgtt
     3241 tcatagtccg aaacaccgac aacatccgga ccgatactgc agttggcaaa aataaatacg
     3301 gtggcaaata gtatgacaaa caggcatgca ttctttttca ttgcgggcct ccgacaatgg
     3361 actcaggttg gaaggaggag cccctcccca tccccggtac agtgagtata aattccaccg
     3421 gggacggggc ccatggggga aattccctat tttacataga tgtcggatgt accatttttc
     3481 ttgacaaaaa gccgccttct ccggatctgc tccaacctgt gaaattcccg catgcctgaa
     3541 tcaatccccc tccggtggag ccgacaggaa gacccagtag tgacgatacg tcgatcgggc
     3601 gtcataatag tggccgatcg caacgacggt ctggcctgca taaaaaccga tttcggccag
     3661 gacgtgtttt ttatgccccg gggaattcat ccagccatca taggcggccc gggccgtgct
     3721 tctccctccg ctgatggact cgatattgtt ggcattccgc gccgtacccc accaagaggg
     3781 caaacggtat cccgcacgtt tgagcaggat attgggccca tccccgtcgg gattggtatg
     3841 cgaaaaatag ttccgttgtg ccatgtcttt tgcccgttcc cgtgccacac gctgcagaac
     3901 aggattgagt gtcaaccggg tccgcttctg ccgcgggtca gcccgcagga tacgcatgaa
     3961 atcctgctcg atctgcgtca gcgagacatt gccgctgctc tcgttcctcc cgacttcccc
     4021 ggttccctga gtgacagccg gtttcccgcc cgtcatgttc cccgtacacg ccagcatcgc
     4081 gccaagcaaa acaaccaaca ttcctgcacg cattttttga tcccccttgc tgtcaatctt
     4141 tccgatctta ccgacaccac ttctgttgca tacctcaaca gtccgttttt ggatgtccct
     4201 gtcggaccgc cccccgggca atgcagcgcc agatacagcc cgggacatcc atgatggatc
     4261 gtgcttcggc aaaggcaccc gccaacggac caaagacgat caggggaacc ggattggtgg
     4321 tatgggcccg tgtcgcacaa tcctcgatgt tcccgtggtc actgatgatg acaagggtac
     4381 cgccatccgc gaccaccgcc tccgccagcc tcgtgagaaa tctgtcgagg accgataaaa
     4441 aatccctggc ttccccgact tgtgccttgt gaccgagcat gtccgtcatg aaggtttcat
     4501 agaggaccaa cccgtgatcg cgcgcaatct ccgcgagatg ccgcgctgcc tcttccggtt
     4561 ctgtttgagg aacatcctca cgcccagtcc gggtttggac catcgcccgg gtgatgtccc
     4621 agtacacggc acgaccggcc acaagatcct ccagcatcct gaaagggatc ccg
//
LOCUS       DAOSHE010000124         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16441_length_4666_cov_4.480807, whole genome shotgun sequence.
ACCESSION   DAOSHE010000124 DAOSHE010000000
VERSION     DAOSHE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16441_length_4666_cov_4.480807"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..460)
                     /locus_tag="PLD82_04680"
     CDS             complement(17..460)
                     /locus_tag="PLD82_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64716.1"
                     /translation="MLRITCTILLLAFCGLLSADNEELRPLDRAPDISGQWYYGIDSN
                     PEFIVRVSQTPAGVTARWYIRDPEEGSELAYAEAQGRFMRFHEILMHGRYLRWFQGTP
                     PGERFTAHWIIYAGKTRMQEISMSGNNRITSYLVHGFRPWQSDHP"
     gene            585..1325
                     /locus_tag="PLD82_04685"
     CDS             585..1325
                     /locus_tag="PLD82_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64717.1"
                     /translation="MSMRVTMLRLVSSVIVAALSPVVLVAQSSATNRKVVPATNAKPA
                     TVQTNRTAALEARVRYLEAEVAKLWRYAAWVKSQIPGEQTIARIADTRASIQVNRYLG
                     GKSDDPTRMPLTASEGGVVLPHIVVGNDRVVEPMVAAGLRIAIAARGPKVNGNALVTV
                     YALPLADPVLRPAWLVHADGNVPYLDGVTQAHFFWKGHFVDGKKAPAGKYRLFVRVIA
                     SETSGKRIGGAMRWWGGTDAASSVIEIK"
     gene            1378..1854
                     /locus_tag="PLD82_04690"
     CDS             1378..1854
                     /locus_tag="PLD82_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG64718.1"
                     /translation="MNITEVITEEYICPALVSEDKDELFAELVEVLVRARPGLDREAI
                     LKAIREREVKMSTGIGKGIAIPHGKTNVVDDLCGVIGISRKGVEYDALDGEPVHVVFM
                     LLAPMTSAGRHIKALQKIANLMKDRGCYQRLCDAKSSAEVWQIIHADEERLADSGD"
     gene            1857..2174
                     /locus_tag="PLD82_04695"
     CDS             1857..2174
                     /locus_tag="PLD82_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64719.1"
                     /translation="MQEIVQRMLEVEREAASRIATAEAAAAKRLEDARNEADHLLNQA
                     RQEAAAEARELVDAAREEAGQERSERVREAMQDLEDRLKTLQDKRESVVQRMADVLFQ
                     GEQ"
     gene            complement(2227..2877)
                     /locus_tag="PLD82_04700"
     CDS             complement(2227..2877)
                     /locus_tag="PLD82_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64720.1"
                     /translation="MRSCQIPFRGTKSLPANLHEPTTPPRALLVLAHGAANTGKETAL
                     IVDLARELVSINLAVLRFDFPFVADGKKPPNSEPVLVEACLAALDEGRRILPGIPVLV
                     GGKSLGAKIALSSARNNPAGAILLGFPLHAPGHTGTEAATYLQVFQRPVLIIQGTRDA
                     FANPALLKSVTDPLKHVTLLPIEGGDHSLRAAGGHQSAIEQAVRATDLWLRKKLSL"
     gene            2926..3010
                     /locus_tag="PLD82_04705"
     tRNA            2926..3010
                     /locus_tag="PLD82_04705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2960..2962,aa:Ser,seq:cga)
     gene            3028..3112
                     /locus_tag="PLD82_04710"
     tRNA            3028..3112
                     /locus_tag="PLD82_04710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3062..3064,aa:Ser,seq:gga)
     gene            3196..3293
                     /gene="ffs"
                     /locus_tag="PLD82_04715"
     ncRNA           3196..3293
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD82_04715"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3326..>4666
                     /gene="dnaX"
                     /locus_tag="PLD82_04720"
     CDS             3326..>4666
                     /gene="dnaX"
                     /locus_tag="PLD82_04720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG64721.1"
                     /translation="MQETGFRATALRYRPQTFAGLIGQEHVSQTLTNAIKSGRVAHSY
                     LFSGPRGVGKTSAARILAKSLNCEHGPTDTPCGVCTNCIEIAESRSLDVFEIDGASNR
                     GIEQIRDLRESVRFTPARSRYKIYIIDEVHMLTNEAFNALLKTLEEPPPYIVFVFATT
                     EAAKVKITIRSRCQHYRFKRIQTREIQARLRAVCETDGIPAEEEALFLIARAADGSMR
                     DSQSLFDQVASYALDGITAVHAREIIGSIDTAAYFDFLTFLLERDSAELLARIAAMDD
                     EGVDFIAFAQGLVEMLRNLVLLASGADEKTLELTPEDCNDLKSFVDPSSSDGFSQDDA
                     VTLLNILADLMSELHDARNPRRVFEMAVFRLVNFRNLVRPEDVLRRIEDLERRLRGDA
                     AHSGELVRKPGPGMPIEIAVVPPAPATRPFTPVAAPQPKPVPVEEPAPGQMPVPP"
BASE COUNT          964 a         1259 c         1477 g          966 t
ORIGIN      
        1 ggtgcagaaa ttctgttcag ggatggtcgg attgccaggg ccggaatccg tggacaagat
       61 agcttgtgat gcggttgttt ccggacatgc tgatttcctg catccgggtc ttgccggcgt
      121 agatgatcca gtgcgcggta aaccgctcgc cgggtggagt tccctggaac cagcgcaggt
      181 atcggccatg catgaggatc tcgtgaaagc gcatgaacct cccctgtgct tcggcgtagg
      241 ccaactcgct tccctcttcg gggtcacgga tataccagcg agccgtcaca ccagcgggtg
      301 tctgtgagac acggactatg aactccggat tggagtcgat tccatagtac cattggccgc
      361 tgatatcagg agcccgatcc agtggccgga gttcttcgtt gtccgcggag aggagtccgc
      421 agaatgcaag gagcaggatg gtgcaggtga tccggagcat acggaatatc tatcggtcgg
      481 atgtatgaga attttctctt gtttttgcac tcccgtcagt attttacatt gatgagcgca
      541 atcaggcgct tgctcctgga ttccatggag ccatggaggt ctcgatgtcc atgcgcgtca
      601 ccatgcttcg gcttgtgtcg tccgtcattg tcgctgcgct ttctcccgta gttcttgttg
      661 cgcaaagttc cgccaccaat cgcaaggtcg tgccggccac aaacgcaaaa ccggccaccg
      721 tacagacaaa ccggacggca gcacttgagg cccgtgtccg gtatctcgag gcagaggtcg
      781 caaaactctg gcgctacgcc gcctgggtca agtcccagat ccccggagag cagaccattg
      841 ccaggattgc ggacacccgg gcgagcatcc aggtcaacag atacctgggt ggcaagagtg
      901 acgacccgac acggatgcct ctgacagcct ccgagggggg tgttgttctg ccacacattg
      961 ttgtcggaaa cgaccgtgtg gttgagccga tggtcgccgc cggactcagg attgcgattg
     1021 ctgcccgagg gcccaaggtc aacggaaatg cccttgtgac cgtctatgcg ctcccgctgg
     1081 cggatccggt tctcaggcct gcttggcttg tgcatgccga cggtaacgtt ccctatctgg
     1141 atggagtcac ccaggcccat ttcttctgga aggggcattt tgtggacggg aaaaaagcgc
     1201 cggccgggaa gtaccggctt tttgtgcggg tgatcgccag tgagaccagc ggaaagcgga
     1261 ttggtggtgc catgcgctgg tggggcggaa ccgacgctgc aagcagtgtc atcgagatca
     1321 agtagccaat caggggtgtt gccttccctg tcagtcaatc agcataagga agtggatatg
     1381 aatatcacag aagtcatcac agaggagtac atctgcccag ccttggtcag tgaggacaag
     1441 gacgaactct tcgccgaact ggtcgaagtc cttgtccgtg ccagaccggg tctggaccga
     1501 gaagcgatcc tcaaggccat ccgtgagcgt gaagtcaaga tgtcgaccgg aatcggcaaa
     1561 gggattgcca ttcctcacgg aaaaaccaat gttgtggatg acctctgtgg tgtgatcggt
     1621 atctcccgca agggcgtcga atatgacgca ttggatggcg agccggtcca cgtggtgttc
     1681 atgcttctgg ctcccatgac cagtgccgga cggcatatca aggcgttgca gaaaatagcc
     1741 aatctcatga aggatcgggg atgctatcag aggctctgtg acgccaagtc ctcggccgag
     1801 gtgtggcaga tcatccatgc ggacgaagaa cgcctggctg attccggaga ctgagcgtgc
     1861 aggaaatcgt tcagcgcatg cttgaggttg agcgggaggc ggcttcccgg atagccacag
     1921 ccgaggccgc tgcggccaag cgtctcgagg atgcgaggaa tgaggcggat catctgctca
     1981 accaggcccg gcaggaggct gcggccgagg cacgtgagct tgttgatgca gcccgcgaag
     2041 aagcgggaca ggagcggagc gagcgggtcc gcgaagcgat gcaggatctt gaggatcggt
     2101 tgaaaacgct tcaagacaag cgggagagtg ttgtccagcg gatggcggac gtcctcttcc
     2161 agggagaaca gtaagagcca acaggcatga cgcctgtatg cagagcagtc tcttgcctga
     2221 aacatttcac aacgagagct ttttcctgag ccagaggtcg gtggcacgca cggcttgttc
     2281 aatggcggat tggtgcccgc ctgcggcacg cagggagtgg tctcctccct cgatcgggag
     2341 gagtgtgacg tgtttgagcg gatccgtaac gcttttgaga agggccggat tggcaaaggc
     2401 atctcgtgtt ccctggataa tcaggacagg ccgctggaaa acctgcagat atgtggctgc
     2461 ttctgtgccg gtgtgcccgg gagcgtgcag gggaaatccg aggaggatgg caccagccgg
     2521 attgttgcgg gctgatgaaa gcgcgatttt ggccccaagg gacttgccgc cgacaagaac
     2581 cggaattccg ggcaggatgc gacgaccctc gtcgagggct gccaggcagg cttcgacaag
     2641 caccggttcg ctgttcgggg gttttttccc atcggccacg aacggaaaat caaaccgcag
     2701 gacagcgagg ttgatggaga ccaattcccg ggcgagatcc acaatcaggg ccgtctcttt
     2761 gccggtattg gcggcaccat gggccaggac caaaagcgcc cgcgggggag tggtcggttc
     2821 atggaggttt gctggaaggc tttttgtgcc gcgaaagggt atctggcagg atctcatacg
     2881 gagaatctat tgatttcagg ggagctatca agtatattct tcgacggaga ggtgcgagag
     2941 tggttgaatc ggccggtctc gaaaaccgtg agtccgcaag ggcacggggg ttcgaatccc
     3001 cccctctccg cagaagtgtg ttccattgga aaggtgaccg agtggccgaa ggtgcttgct
     3061 tggaaagcaa gtgtagggca acctaccggg ggttcgaatc cccccctttc cgaatttctc
     3121 ccatggtgag tggttctgtt gtctgcggtg cttggtagca ggcgctcatc cacagggctt
     3181 ttttgctgcg ccgtagtcca ggtcctgttc aatcggaccc agcccgaacc cgtcaggtcc
     3241 ggaaggaagc agcggtaggt tggtgctgat gtgtagcagt caacctggac tatggggccg
     3301 caaaagggta catgaggagg gacaggtgca ggagaccggc ttcagggcga cagcactacg
     3361 gtaccgaccg cagacctttg cgggcttgat cggccaggaa cacgtttcac agacgctgac
     3421 caatgccatc aagagcgggc gtgtcgccca cagttacctc ttttccggac cacgtggtgt
     3481 tggcaagacc tctgccgccc gcattctcgc caaatcactc aactgcgaac acggaccgac
     3541 ggatactccc tgcggagtgt gtacaaactg catcgagatc gccgaaagcc gctcgttgga
     3601 tgtattcgag attgacgggg catccaaccg tggtatcgaa cagatccggg acctccgcga
     3661 gagtgtccgg tttacaccgg cccgcagccg gtacaagatc tacatcatcg acgaagtcca
     3721 catgctgacc aacgaagcct tcaacgcctt gttgaagacg ctcgaagagc cacccccgta
     3781 tatcgtgttt gtttttgcga ccacggaggc ggccaaggtc aagatcacga ttcgttcccg
     3841 atgccagcac taccgtttca aacgcatcca gaccagggag atccaggccc ggctgcgggc
     3901 tgtgtgcgag acggacggga tccccgccga ggaagaagct ctctttttga ttgcccgggc
     3961 cgctgatggg tcgatgcgcg attcccaatc gctcttcgac caggtggcga gctacgcgct
     4021 ggacgggatc actgcggtac atgcgcggga gatcatcggt tccattgata cagcggccta
     4081 tttcgatttt ctcaccttcc tgctggagcg ggacagcgcc gagctgttgg ccaggattgc
     4141 ggccatggac gacgagggcg tggactttat cgcttttgcc cagggtctgg tcgagatgtt
     4201 gcggaatctt gtgctgctcg cctcgggggc tgacgaaaaa acactggaat tgacgccaga
     4261 ggactgcaat gacctcaaga gttttgttga tccctcgtct tcggatggct tttcccagga
     4321 tgatgcggtc acactcctga acatcctggc ggatttgatg agcgagctgc atgacgcgcg
     4381 gaacccgaga cgtgttttcg agatggctgt tttccgcctt gtcaatttca ggaacctggt
     4441 caggcccgag gacgtcctgc gccggatcga ggatctcgaa cggagattgc gcggggatgc
     4501 ggctcattcc ggtgagttgg tgcgcaagcc aggtccgggt atgccgatcg agatagctgt
     4561 tgtgccgccc gcgccggcaa cccggccgtt cacaccggta gccgcgcccc aacccaagcc
     4621 tgttcccgtc gaagaaccgg caccagggca gatgcccgta ccgccc
//
LOCUS       DAOSHE010000125         4650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16517_length_4650_cov_4.341458, whole genome shotgun sequence.
ACCESSION   DAOSHE010000125 DAOSHE010000000
VERSION     DAOSHE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16517_length_4650_cov_4.341458"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..2110)
                     /gene="fusA"
                     /locus_tag="PLD82_04725"
     CDS             complement(26..2110)
                     /gene="fusA"
                     /locus_tag="PLD82_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG64722.1"
                     /translation="MAREIELKKMRNIGIMAHIDAGKTTTTERILYYTDKEHKIGEVH
                     DGAATMDWMVQEKERGITITSAATTCAWRDCRINIIDTPGHVDFTVEVERSLRVLDSA
                     VAVFCAVGGVQPQSETVWRQANKYRVPRMVFVNKMDRVGADFNRVVKMIRERLKATPV
                     PLQIPVGQEDKFKGVIDLLKMKAFIWHGEENGAKFEEIEIPADLHEDAVLAREHMIEC
                     VCEADDVLIEKYLEGIEVTNEELMEGIRRATIGMRIFPVVCGTAFKNKGVQQLLDAVV
                     EYLPSPIDIAPAEGINPKTDAEDTRPSSDDAPFAALAFKIQTDPYVGKLCYFRVYSGK
                     ITSGTQVLNSTKGSKERIGRLLQMHSNKREERDEAWAGDIMAAVGLKNVTTGDTLCDD
                     ANPIILESMVFPEPVINIAIEPKTKSDQDKIGVALSKLAEEDPTFRVRVDPETAQTII
                     AGMGELHLEIIVDRLLREFKVEANIGKPLVSYRETIRKSVEIENKYVRQTGGHGQYGH
                     VVMTVEPNETGKGFEFVNKIVGGVIPKEFIPAIEKGVREAVDSGPLAGYPLVDVKVTL
                     TFGSYHDVDSSEMAFKICASQALKEGANKANPALLEPIAKIEVVTPEDYMGDVMGDLN
                     SRRGRIIGMEDQNGAKAISAHVPMSDMFGYATDLRSKTQGRASYTMQFDHYSEVPKNI
                     SEEIIAKVSGGK"
     gene            complement(2245..2715)
                     /gene="rpsG"
                     /locus_tag="PLD82_04730"
     CDS             complement(2245..2715)
                     /gene="rpsG"
                     /locus_tag="PLD82_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG64723.1"
                     /translation="MPRKSYKKHDWLQPDSRFNSLQVAKFINKLMFDGKKSVAEAIFY
                     SAMDLVKEKTGEEPLEVFMKAFENAKPTVEVKSRRVGGANYQVPVEIRPERRDALAFR
                     WIINFSRARGEKGMHVRLANEIIDASKSTGATIKKKEETHRMAEANKAFSHFRW"
     gene            complement(2738..3112)
                     /gene="rpsL"
                     /locus_tag="PLD82_04735"
     CDS             complement(2738..3112)
                     /gene="rpsL"
                     /locus_tag="PLD82_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG64724.1"
                     /translation="MPTVNQLVRKGRKSLHSKTKSPALQSCPQRRGVCTRVMTVTPKK
                     PNSAMRKVARVRLTNGIEVTAYIPGIDHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DTHGVKNRCQGRSKYGAKKPKA"
     gene            complement(3298..>4650)
                     /locus_tag="PLD82_04740"
     CDS             complement(3298..>4650)
                     /locus_tag="PLD82_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG64725.1"
                     /translation="RAPQHANVSVDKRVRLLLTSVADNVLPVKVGSMLFVEEAEEVKA
                     GSPIAKFDPYSEPIIAEVTGKVVYENMKLGQNVKETIDEKTGRKQLVALEARKKNEEQ
                     LKIRISGKSEEREYMMPGGAILVVEDGSQISLGDTIARTPREQTKTKDITGGLPRVAE
                     LFEARRPKDPAIIAKIDGTISIESQISKNKRIVLVDDGDRDENGNIPDDVYHKYPIPV
                     GKHLLVRNGDDVKAGDQLCDGAIDPHDILEVKGEQELMRYLMNEVQEVYRLQGVKIHD
                     KHIGVIIRQMLRKVEITDVGDTDFIIEQKVNRNIFRRKNEETIARGGQPAQAKPILLG
                     LTKASISIESFLSAASFQETTKVLTNAAIQGRSDCLRGLKENVIIGHKIPAGTGMDIY
                     DELEMEWPQEEAYVVHEARQSDEYVLSREIGVYQEQEAEFASRGYTIEDGPGGATDDF
                     "
BASE COUNT         1022 a         1324 c         1111 g         1193 t
ORIGIN      
        1 actaccttat agcatgaaat gctatttatt taccgcctga cactttcgcg atgatttcct
       61 ccgaaatatt ctttggaact tcgctatagt ggtcaaactg catcgtgtaa ctggcgcggc
      121 cctgggtctt cgagcgaaga tctgtcgcat acccgaacat atcgctcatg ggaacatggg
      181 cgctgatcgc cttggcgcca ttctggtctt ccatgccgat gatcctgcca cgtctggagt
      241 tgaggtcgcc catgacatca cccatatagt cctcaggtgt gacaacctca atcttcgcaa
      301 tcggctcgag caaggccgga ttggccttgt tggcgccttc tttgagtgcc tgggaagcgc
      361 agatcttgaa agccatttcg gaagagtcca catcgtgata cgaaccgaaa gtcagagtga
      421 ccttgacatc cacaagcgga taccctgcca acggaccact gtcgacggcc tcgcggacac
      481 ccttttcaat agcggggatg aattccttgg gaataacacc gcccacgatc ttgttgacaa
      541 actcgaagcc cttgccagtc tcgttgggct cgacagtcat cacaacgtga ccgtactgac
      601 catgaccacc tgtctgcctg acatacttgt tttcgatctc gaccgacttc ctgatcgttt
      661 cacgatagga aacaaggggc tttccgatat tggcttcaac tttgaactca cgcaacagac
      721 ggtcgacgat gatttccaag tgcagctcac ccatacccgc gatgatcgtc tgtgctgttt
      781 ccggatcgac cctgaccctg aatgtcgggt cttcttcggc aagcttcgaa agagccacac
      841 caatcttgtc ttgatcgctc tttgtcttgg gctcgatcgc gatattgata accggctcgg
      901 ggaagaccat cgattcaaga atgatcgggt tggcatcatc gcagagagta tcgccggttg
      961 tgacattttt gagcccgaca gcggccatga tatcgcccgc ccaggcctca tcccgctcct
     1021 cacgcttgtt ggagtgcatc tgaagcagac gaccgatgcg ctccttgctc cccttggtgg
     1081 agttaagcac ctgggtgccg gatgtgattt ttccggaata gaccctgaaa taacagagtt
     1141 ttccgacata aggatcggtc tggatcttga aggccagagc cgcaaacgga gcatcatccg
     1201 aggagggccg ggtatcttcg gcatccgtct tggggttgat cccctcggct ggagcaatat
     1261 cgattggaga aggaagatac tcgacaacag catcaagcaa ttgctgaaca cccttgttct
     1321 tgaaagccgt tccgcacacg acgggaaata tccgcattcc gatcgtcgcg cgcctgatgc
     1381 cttccataag ttcctcgttg gtaacctcaa ttccctcgag atacttttcg ataagcacat
     1441 catccgcttc acagacacat tcaatcatgt gctcacgtgc aaggacagca tcctcgtgca
     1501 gatcagccgg aatctcgatc tcctcgaatt tggctccatt ttcttcacca tgccagataa
     1561 acgccttcat cttgagcagg tcaataacgc ctttgaactt atcttcctgc ccgacgggaa
     1621 tctgtaatgg aaccggtgtc gccttgaggc gctcacggat catcttgaca acacggttga
     1681 agtcggcacc cactcggtcc atcttgttga caaaaaccat acgcggaacg cgatacttgt
     1741 ttgcctgacg ccagaccgtt tcgctctggg gttggacacc gccaacggca caaaaaaccg
     1801 caacagccga atcaagcaca cgaagagaac gttcgacttc aactgtgaag tccacgtggc
     1861 cgggggtatc gatgatattg atccggcaat cacgccaggc gcatgtcgta gccgcactgg
     1921 taattgtaat accacgttcc ttctcctgaa ccatccagtc catcgtggct gcaccatcgt
     1981 ggacttcgcc gatcttgtgc tctttgtctg tataatacaa gatacgttca gtcgtcgtcg
     2041 tcttaccggc gtcaatatgg gccataatcc cgatattgcg catctttttc aattcaatct
     2101 ctctagccaa accgatcctc cagaatcttc aagccaaaaa acatccgtac atgcgtacac
     2161 aaaacactgc acccattgcc attgcatgga tgcagtgccg gcgaaatctc caaacccagg
     2221 caagcgccag aaacaaaaaa cagcctacca cctgaagtga gagaacgcct tattggcctc
     2281 tgccatacga tgcgtttctt ccttcttttt gatcgtagca cctgtgctct ttgaggcatc
     2341 tatgatctcg ttggcaagac gaacatgcat gcccttttca ccgcgcgcac gcgagaaatt
     2401 aatgatccaa cggaatgcga gagcatcccg cctttcaggg cgaatctcga caggaacctg
     2461 gtagttggca ccaccaacac gccttgactt gacttcaacg gtcggcttgg cgttttcaaa
     2521 agccttcatg aagacctcga ggggttcctc ccccgtcttc tctttgacaa gatccattgc
     2581 cgaataaaaa attgcttcgg caacactttt cttgccatcg aacatcagtt tattgatgaa
     2641 tttggctacc tgcagcgagt tgaatcgcga atcgggctgc agccaatcat gctttttata
     2701 cgactttctg ggcacggtat ctactccttg ctctttctca ggcttttggc tttttagcgc
     2761 cgtacttcga acgaccctgg cagcggtttt tcacgccgtg ggtatcgaga gtaccgcgga
     2821 caatgtgata acggacaccg ggaaggtcct tgacacgacc accacggatc agcacaacgg
     2881 agtgttcctg gaggttgtga tcaatacccg ggatgtaggc tgtcacttca attccgtttg
     2941 tcaagcggac acgggctact tttctcatgg ccgagttcgg ctttttcggg gtgactgtca
     3001 tcacccttgt gcaaacgcca cgacgctgcg ggcatgattg cagagccggc gatttggtct
     3061 tggaatgcag ggactttcgg cccttgcgaa caagctggtt tactgtaggc atttcctgat
     3121 ccttgtgcgt tctcttttac aagttatgtg aatttacgta ctggagaaag aaaggtcaag
     3181 aaacaaccct ccgtttctcc gcacttttct tactcgtcct ggttttcctc gtcgatatcg
     3241 tcgtcaatct cgtcgatttc atcatcgata tcgtcgtcgt catcgtcgtc atcgtcctta
     3301 aaaatcgtcg gtcgctccgc ccgggccatc ctcgatcgta tagccccgac ttgcaaattc
     3361 ggcttcctgc tcctgataga ctccgatctc ccggctaagg acatactcat cggactgacg
     3421 tgcctcgtgg acaacatagg cctcctcctg tggccattcc atctcgagct cgtcgtagat
     3481 atccatgccg gtaccggccg gaatcttgtg cccgataatg acgttttcct tgagtccgcg
     3541 aaggcaatcc gaccttccct gaattgccgc gtttgtcagc acctttgtcg tttcctggaa
     3601 ggaagcagcc gaaaggaagc tttcgatcga gatggatgcc ttggtcaaac caaggagaat
     3661 cggtttggcc tgtgcaggtt gtccgccccg ggcgatcgtc tcttcgtttt tacgcctgaa
     3721 aatattccgg ttgaccttct gttcgatgat gaagtccgta tcaccgacat ccgtgatctc
     3781 gaccttgcgc aacatctgac gaataatgac accgatgtgc ttgtcatgaa tcttcacacc
     3841 ctggaggcga tacacctcct ggacctcatt catgagataa cgcatcaact cctgctcgcc
     3901 cttgacctcg agaatgtcat ggggatcaat ggcgccatca cagagctgat cgcccgcctt
     3961 aacatcgtct ccgttgcgaa cgaggaggtg ttttccgaca ggaatcgggt atttgtgata
     4021 gacgtcatcg ggaatgttcc cgttttcatc ccggtccccg tcatcaacaa gaacgatacg
     4081 cttgttcttg gaaatctggc tctcgatgct gatcgttccg tcaattttgg caatgatggc
     4141 cggatccttc ggtctccgtg cctcaaagag ctcggcaacg cgtggcagac ctccggtgat
     4201 gtcctttgtc ttggtctgtt cgcgcggagt acgggcaatg gtgtcaccaa gactgatctg
     4261 gctgccatct tcaacaacga ggatggcccc accaggcatc atgtactcgc gctcctcgga
     4321 cttgccggag atgcggatct tgagctgttc ctcattcttt ttgcgtgctt ccagggcgac
     4381 caactgtttg cgtcccgtct tctcgtcgat cgtctctttg acgttctgcc ccagcttcat
     4441 attctcgtag acaaccttgc cggttacctc ggcaatgatg ggctcggagt atggatcgaa
     4501 cttggcaatc ggagaaccgg ccttgacctc ttccgcttct tccacaaaca gcatggaacc
     4561 aaccttgacc ggcagaacgt tgtcggccac gctcgtcaac agcagccgga ccctcttgtc
     4621 cacggaaaca ttggcatgct ggggagcacg
//
LOCUS       DAOSHE010000126         4648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16534_length_4648_cov_4.338559, whole genome shotgun sequence.
ACCESSION   DAOSHE010000126 DAOSHE010000000
VERSION     DAOSHE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16534_length_4648_cov_4.338559"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLD82_04745"
     CDS             complement(<1..489)
                     /locus_tag="PLD82_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64726.1"
                     /translation="MRLVSFFPAVCLVLLACPLGAVLDPVTPRLPVIPNGSGDGQETN
                     RTTIVTNPQAPATGTRYRVRVWFVNGSSLEGSAVFPFSRYTVEQTIGGRRYYRTLTLD
                     ETGSIRVRRWVPVIMSSQGKELYYFLPGLYRILPKTGTELVLDKRMKMLDSFDIATPA
                     GVT"
     gene            complement(486..1154)
                     /locus_tag="PLD82_04750"
     CDS             complement(486..1154)
                     /locus_tag="PLD82_04750"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG64727.1"
                     /translation="MQSAEENELEVVLEEMDEDAVLEFLLNADGPVFESVLIRLAWPP
                     ADDPSFVYTRHFALYHRLASWQARFHREGKYLHMDCMRIRLLPYPHPGTCRYYDDRNG
                     SFCASLALPGVNACSRHQGMVDGIFLAPLAGYYLEKEHHAADRSAVLARYQGAGPLTE
                     LLAAYNDLGVSPDSEWQTVRKQYHDMVFLCHPDTGSGDAERLARIQAAWRRISKAHEA
                     RRVE"
     gene            1262..2458
                     /gene="tyrS"
                     /locus_tag="PLD82_04755"
     CDS             1262..2458
                     /gene="tyrS"
                     /locus_tag="PLD82_04755"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG64728.1"
                     /translation="MTDFLNVLKERGFIAQISDEPGITKYLGTPRTAYCGFDPTADSL
                     HVGHLVPIMALSWFEKTGHRPIAIVGGGTAMIGDPSGRTELRKMLTVDQIMANAESIK
                     MQLSRYMTFSDTGAILVNNADWLRNFNYIDFLRDIGRHFSVNRMIAAETYKARLEHGL
                     SFIEFNYQILQAYDFLHLCQNHGCRLQMGGNDQWGNIVAGIDLIRRIDEKESYGLTFP
                     LITTASGAKMGKTSAGAVWLDAGRTTPYDYYQFWRNTEDADVGRFLALFTCLPMDEVR
                     RLAALQGEECNEAKKILALEATAVTHGRPAAETAADWKANMPETVLPASALNDQLPLL
                     ELLVTVGACASKGEGRRLVQQGGIMINDSAVTAIDRVVTESDFQARQLVIRVGKKKFY
                     RVVLNS"
     gene            2455..3186
                     /locus_tag="PLD82_04760"
     CDS             2455..3186
                     /locus_tag="PLD82_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG64729.1"
                     /translation="MKKKSRILVIIPTYNESMNVKVITPLVLAVDPRIEILFIDDNSP
                     DGTAAILHAMIKRNKRIHILERPGKLGLGTAYVTGFQYALEHGYDYVFEMDADLSHDP
                     KYLAAMIHTVEHEADVVVGSRYIHGVSVAHWPLKRLLLSKLANVYASFFTGVKVRDLT
                     AGFVGYRNTVLADIDVASVRSSGYAFQIELKYRCHKQGYSIKEIPIIFADRERGESKI
                     SKGIIFEALHRCISLRWTYRPRRKK"
     gene            complement(3197..3895)
                     /locus_tag="PLD82_04765"
     CDS             complement(3197..3895)
                     /locus_tag="PLD82_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64730.1"
                     /translation="MTGIVLAGLLIQGVLMLFSRTAAVFPGAFASLVLMLISAGKMSR
                     GFPLEFLGMEVPAWAALLVGLAWFGFSVWCLFRLEKAFIPATFKGLVLGLGKAALLFS
                     IVIVLMAVIVFPMVLLATSLPGIFSGLVLGGGLVLMVWILVRKYRTMIRLDIADTRVR
                     SREDWPSLGLRWLFHTAVRPFLILAALVAYVYGLALFGVAPFFGGLILVVLTLACMWL
                     TKAVRALPWMGGSE"
     gene            4019..4507
                     /locus_tag="PLD82_04770"
     CDS             4019..4507
                     /locus_tag="PLD82_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG64731.1"
                     /translation="MAPSVTIKDRIIQYMNQTLLSTLVTVVHGRPVSRMMLFGNDKRG
                     TVWLCTRADSSILDEIRQFPHVALSIFREETLLDEIVSLSVEGEARIVEDPMDRLSLE
                     GFDILGRKSPRIGDIPHAGEADRYRIVIVSPTLFRFHSWADHVKGVPPVTLRRKSGAS
                     TI"
BASE COUNT         1148 a         1392 c         1218 g          890 t
ORIGIN      
        1 cgtcacgccc gctggtgtgg caatatcgaa actatccagc attttcatgc gtttgtccag
       61 aaccaattcg gtacctgttt ttggaaggat tcgatacagc cctggcaaga aatagtagag
      121 ttccttgcct tgcgaagaca tgatgacagg aacccagcgc ctgacacgga tgctgccggt
      181 ttcatcaagc gtcaatgtcc ggtaatagcg tctccccccg atggtctgtt cgaccgtgta
      241 tcgggaaaaa ggaaaaaccg cacttccctc aagactgctt ccgttcacaa accagacgcg
      301 aacccgatag cgtgtccccg tggccggcgc ctgcggattc gtaacgatcg tggtcctgtt
      361 tgtctcctgc ccgtccccag aaccattcgg aatcacggga agccgcgggg tgacaggatc
      421 aaggacagca ccgagcggac atgccaggag caccaggcag accgccggaa aaaaagaaac
      481 aagcctcact ctaccctccg cgcttcatgt gccttgctta tccggcgcca ggcagcctga
      541 atccgtgcca gtcgctccgc atccccggaa cccgtatcag ggtggcacaa aaacaccata
      601 tcgtgatact gtttccgtac cgtttgccat tcagaatccg gtgaaacccc cagatcattg
      661 taggccgcaa gaagttccgt gagcggaccc gcaccttgat agcgggcaag cacggcactc
      721 ctgtcggcgg catgatgctc cttctcaaga taatacccgg ccaggggtgc cagaaatatc
      781 ccgtctacca tgccctgatg ccgtgagcag gcgttcaccc cgggcagcgc cagtgatgca
      841 caaaaagaac cattccggtc atcgtagtat cgacaggtcc ctggatgcgg gtatgggaga
      901 agcctgatgc gcatgcaatc catgtggaga tattttccct cgcgatggaa ccgggcctgc
      961 caggatgcca accggtgata caaggcaaaa tgcctggtgt acacaaagga tgggtcgtca
     1021 gcgggtggcc aggccagccg gatcaagaca ctctcgaaga ccggaccgtc cgcattcagc
     1081 agaaattcga ggacggcatc ttcgtccatc tcctccaaca caacctcgag ttcattttct
     1141 tcggcagatt gcaccctgtc tccccctggc gatcttcttg acccggatcc tcaatcggct
     1201 gtagtttaaa gtatacccgt gatttttcgt cccgggacaa ataccggtat gcaggtacac
     1261 catgaccgat tttctcaacg tgctgaagga gcgcgggttt attgcgcaga tctccgatga
     1321 acccggaatc acgaaatatc tcgggactcc ccggacggct tactgcggtt ttgacccgac
     1381 ggcggacagt ctccatgtcg gacaccttgt accgatcatg gcgctctcct ggtttgaaaa
     1441 gaccggtcac cggccgattg ccattgttgg aggcgggaca gccatgatcg gtgatcccag
     1501 cggaagaacc gaacttcgca agatgctgac tgtcgaccag atcatggcaa atgccgaaag
     1561 catcaaaatg cagctctcgc ggtatatgac gttttcagac accggggcaa tcctcgtcaa
     1621 caacgcggac tggttgcgga atttcaacta catcgatttc ctccgtgata tcggccggca
     1681 cttcagcgtg aatcgtatga tcgcagcaga gacctacaaa gcacgccttg aacacggtct
     1741 ctcgtttatc gaattcaatt accagatctt acaagcctac gacttcctcc acctctgcca
     1801 gaaccacggg tgccggttgc agatgggcgg aaatgaccaa tgggggaaca tcgttgccgg
     1861 aatcgacctg atcaggcgga ttgacgaaaa ggagtcctat ggtttgacct tccccctgat
     1921 cacaaccgct tccggagcaa agatgggcaa gacctcagcc ggggcagtct ggcttgatgc
     1981 gggtcggacg acgccctacg actattacca gttttggcga aatacggaag acgcggatgt
     2041 cggtcgtttt ctggccctct ttacctgtct cccgatggac gaggtcagac gtctggccgc
     2101 cttgcagggt gaggaatgca acgaggccaa aaagatcctg gcccttgagg caaccgccgt
     2161 cacccatggc aggcccgccg ccgaaaccgc ggccgactgg aaggcaaaca tgccggaaac
     2221 cgttctgcct gcctccgctc tcaatgacca gcttccgctt ctcgaactcc ttgtgaccgt
     2281 cggtgcctgt gcttccaagg gagagggccg gagacttgtc cagcagggtg gcatcatgat
     2341 caacgacagc gctgtcacgg ccatcgatcg agtggtgacc gaatcggatt ttcaggcgag
     2401 gcaacttgtg atacgtgtcg gaaagaaaaa attctatcgc gtggtactca attcatgaaa
     2461 aagaaaagcc ggatcctggt catcattcca acatacaacg agagcatgaa tgtcaaggtg
     2521 atcacccccc tcgtccttgc ggtcgatcca agaatcgaga ttctcttcat cgacgacaat
     2581 tcaccggacg ggaccgccgc catcctgcac gccatgatca agcgcaacaa gcggatccat
     2641 atcctggaac gacccggaaa actggggttg ggaacagcct atgtcaccgg attccagtac
     2701 gcactcgagc acgggtacga ctacgtcttc gagatggacg ccgatctctc gcacgatccc
     2761 aaatacctgg cggccatgat ccacactgtc gagcatgagg cggatgtcgt cgttggctca
     2821 cggtacatcc acggggtgag cgtggcccat tggccactca agcgtcttct gctcagcaaa
     2881 ctggccaacg tctatgccag ctttttcaca ggggtcaagg tcagggacct gacagccgga
     2941 tttgtcggat atcgcaatac cgtactggcg gatatcgatg tagcctcggt ccggtccagc
     3001 ggctacgcct tccagattga actcaaatac cgctgccaca agcaggggta ctccatcaag
     3061 gagattccca tcatctttgc cgaccgcgaa cggggtgaat caaaaatctc aaagggaatc
     3121 atctttgagg cactgcatcg ctgcatctcc ctgcgctgga catatcgccc ccgcaggaaa
     3181 aaataggact ctatcactac tcgctaccac ccatccaagg cagtgcgcgc acagccttgg
     3241 taagccacat gcaggccagg gtcagaacca ccagaatcag acctccgaaa aacggtgcga
     3301 ccccgaacaa agccagaccg tagacgtagg cgaccagagc tgccagaatc agaaatggcc
     3361 gcacagccgt atggaagagc cagcgtagtc ccagggaagg ccagtcctca cgggatcgga
     3421 cccgtgtgtc ggcaatatcc agccggatca tcgtgcgata cttgcggacg agaatccaga
     3481 ccatcaaaac cagcccccct ccgaggacca gtccagaaaa aatccccgga agactggtag
     3541 ccagaaggac catggggaag acaatgacag ccatcaggac aatcactatc gaaaagagaa
     3601 gggccgcttt gcccagtccg aggaccaatc ccttgaaggt ggccggaatg aacgcttttt
     3661 ccagacggaa caggcaccag acggaaaaac cgaaccacgc aaggccgacg agcagcgctg
     3721 cccaggcagg aacctccatg cccaaaaatt ccagcgggaa ccctctggac attttcccgg
     3781 ccgagatgag catgaggacc aaggaagcaa aagcacccgg aaacaccgcc gcggtccgcg
     3841 aaaaaagcat caagacaccc tgaatgagca accctgccag tacaattccg gtcatcgtgg
     3901 gaacccgtct ttcatcaatt cacaaaaaac atacatcatg cacgcctcac acgcaaggca
     3961 aatccgtcct cttgcatccg ggaccaatcc gcgttagagt ggttgaatgg aggaagacat
     4021 ggcaccatcg gttaccatca aggaccggat tatccagtac atgaatcaga cgcttctctc
     4081 gacccttgtc acggtcgtcc atggacggcc tgtctcgcga atgatgctct tcggaaatga
     4141 caaacggggg actgtctggc tctgcacccg tgccgattca tcgatccttg acgagatccg
     4201 gcaatttccc catgtcgctc tctcgatctt ccgcgaggaa acacttctcg acgaaatcgt
     4261 ctccctttcc gtcgaaggtg aagccagaat cgtcgaggat cccatggaca gactctccct
     4321 tgaaggattt gacatcctcg gaagaaaatc cccccgcatc ggggacatcc cccacgccgg
     4381 agaagccgac cgctatcgaa tcgtgattgt cagcccgaca ctcttccggt tccacagctg
     4441 ggccgaccac gtcaagggcg tgccgcctgt caccctgaga cgaaagagcg gtgcttcgac
     4501 catctgaatc cagagaggac cacaaggagc aggcaggaga agccggcaca gagcagcaaa
     4561 ggcgtcgtga tagccgccag cgaccggctg acgagggcat cggcgatcgt ggcccattcc
     4621 cccccgccac tcttgagacg gacaatcg
//
LOCUS       DAOSHE010000127         4604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16755_length_4604_cov_4.302704, whole genome shotgun sequence.
ACCESSION   DAOSHE010000127 DAOSHE010000000
VERSION     DAOSHE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16755_length_4604_cov_4.302704"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLD82_04775"
     CDS             complement(<1..231)
                     /locus_tag="PLD82_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOG64732.1"
                     /translation="MIKKVLIANRGEIALRVIRACREMGISTVAVYSTADENCLHVRF
                     ADEQVCIGPPVSGESYLNIPSIISTRDLRGAYD"
     gene            complement(228..686)
                     /locus_tag="PLD82_04780"
     CDS             complement(228..686)
                     /locus_tag="PLD82_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG64733.1"
                     /translation="MEKDTLHAIMDAIGHTSIAECEIVAKNGSIRILRKPGLQVPRLR
                     DDVKPDGNGGAESGSPNLDADDDDLVDVTSSWVGFFYRGTKKDDKPCVKLRDTVKEGQ
                     QIGSVVTMNVVQKLSSPVTGKLVEVLVEDGQPVEYGQPLLRMRIEAQDTP"
     gene            complement(790..1584)
                     /locus_tag="PLD82_04785"
     CDS             complement(790..1584)
                     /locus_tag="PLD82_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64734.1"
                     /translation="MEHTIELRIPSMLRILADLGRHQVTRLVAQTLFDLRQSGEDEND
                     RNWFDAEKINNQMFWDDLMGIVTRDDPEGTERVVRHIYYYRQELSRVVAIPGAPLASA
                     VVLVEPEKGQKLVAGSRIVCDVGLELRPVTINLGLETSRSIPAPAEFHAAALDVHLTG
                     LSDMAYDAIAPETLKAGWEQSPVHYDEMDVRIQNGPSETGSGARKIFLEAGSCGPGTY
                     LVHFSTDMTLTTTILPVLEYPRIDDGVARTEERRVTSNHAARIRVV"
     gene            complement(1666..>2081)
                     /locus_tag="PLD82_04790"
     CDS             complement(1666..>2081)
                     /locus_tag="PLD82_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64735.1"
                     /translation="ATDSVDDLLGGSALPSDEIVSGGTSASANFVPESVEQEESVSEI
                     PVFNTKGQVGSYVYLLSVKSGNQLYFGIKGKPQSTPLDFVLSLGFDNGEVGTEMVTID
                     GLDQRGMGYTRIDLSRYARDLGRTAVSKADVALAS"
     assembly_gap    2082..2181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2182..>4604)
                     /locus_tag="PLD82_04795"
     CDS             complement(<2182..>4604)
                     /locus_tag="PLD82_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64736.1"
                     /translation="GTLLLAVPQIGSLLYTIVLVPLVAVSIFAVACLILIAFGLAVFP
                     SHVLFALTPLQGSFIRRLFRESAQLLGIVRKNFFRYVFVSIPAGLAVAVLVAVPVAII
                     AGGYLLSLLVTDSFGSMTGVMGSVTQQLLAWSQNVVSGVQDPMAALSAVFAAISLAHI
                     FAAMLAPALSFLSCMYFRLYIATHERGRAWQLLVERMRALWGLRPDLADDGQTVIDEV
                     TIPGVDLLSTEDMRSLRDDQEREDATWTDWERDAGNAAEDEAAWLLPAAENDFSDELQ
                     VVDEDEDVLSGYGKGPAPAVAGTALVAGAAAMLASDEFTESPMRSGTGFNDGSVAGDD
                     EERLTLGDVDSPEIDGSTLLDSIESSMPLSDEIVPMDGVEPDEDDRLLLDDLETGVTP
                     VSGNEDDILGSITSSMDIAAEPVPMSGIEPDEDDRLLLDDLETGVTPVSDDEDDILGS
                     ITSSMDIAAEPVPMSGIEPDDDDRLLLNDLDTGSDEDDVLRSITETMSLPDEPVPMDG
                     LKPDEDDVLPELNVEDAGRVSQEVDDLIASLDRDLALNGSVPILDPAVLERENEEIAN
                     LDVDQALGLTDGLPAEQIIPAGEDDQENALRLDDLDSMEGLSSAALDGAMDAIMGKEP
                     SGSDAGLSELTGPAGVQATADTGSIDDLLAEANAVFEGGLSDLDLGTGNNIPAGLEMD
                     TGFLGDEQLDDILDGPASFGTSLEDQVSNDGSVPPEEMGLMSRAGESSMDELLASMDE
                     PDSTSDDLLGGLDTPATDSVDDLLGGSALPSDDLLGGLDTPATDSVDDLLGGSALPSD
                     DLLGGLDTP"
BASE COUNT         1013 a         1396 c         1171 g          924 t
ORIGIN      
        1 atcataagcg ccgcggagat cacgggtgct gatgattgaa ggaatattga gataggattc
       61 accggaaacc ggtggtccta tacagacctg ttcatccgca aagcggacat ggagacagtt
      121 ttcatcggct gtggaataga cagcaaccgt cgagataccc atctcccgac aggcccgaat
      181 gaccctgagg gcgatttcac ccctgtttgc gataagtacc tttttgatca tggcgtatcc
      241 tgcgcttcaa ttcgcattct caagaggggc tggccgtatt caaccggctg cccgtcctca
      301 acgaggactt ccaccagctt cccggtcacg gggctggaaa gtttctggac aacattcatg
      361 gtcacgaccg acccgatttg ctgtccctct ttgacagtgt cccgcaactt gacacagggt
      421 ttgtcgtcct ttttcgtacc gcgatagaaa aacccgaccc aggacgacgt cacatccaca
      481 agatcgtcat catcggcatc aagattcggg cttccggatt cagcaccacc gtttccgtcg
      541 ggtttgacat cgtcccgcag acggggaacc tgcaaaccgg gttttctgag aatcctgata
      601 ctcccgtttt ttgcaacaat ctcgcactcg gcaatcgatg tgtgtccgat tgcgtccatg
      661 attgcatgca atgtgtcctt ttccatgcca atcctccgcc aatttcttta tggccatttt
      721 cacagcattc gggaaatcac gtacgaaacc ggattcccgt gaatcattct ctagcagaga
      781 gactctctgc tagaccaccc tgattcgtgc tgcgtgattg ctcgtcaccc gtctctcttc
      841 ggtgcgtgcc acgccgtcat cgatccgggg atattcaagc accgggagaa tcgttgtcgt
      901 cagggtcata tccgtggaaa aatggacaag ataggtcccg gggccgcagg agccggcttc
      961 aaggaagatc tttcgggcac cgctgcctgt ttctgacggt ccattctgaa tccggacatc
     1021 catttcatca tagtgaaccg gagactgctc ccatcctgcc ttgagggtct cgggtgctat
     1081 ggcatcatag gccatatcac tcagcccggt caaatgcaca tccaaagccg cggcatgaaa
     1141 ctccgccggg gctggaatgc tccggcttgt ttccagcccg agattgatcg tgacaggacg
     1201 aagttcaaga ccgacatcac acacgatccg ggaaccggcc acaagcttct ggcctttctc
     1261 cggctcaacc agaacgacag ccgatgcaag cggtgctccc ggtatggcca caaccctcga
     1321 gagttcctgc ctgtaataat aaatgtgtcg aacaaccctt tcggttccct caggatcatc
     1381 ccgtgtcaca attcccatga gatcatccca gaacatctgg ttattgattt tttcggcatc
     1441 aaaccagttg cggtcgttct cgtcctcccc cgattgtcgt agatcgaaca gagtttgcgc
     1501 gaccaatctg gtcacctggt gcctgccaag atccgccaga atgcgcaaca tcgaaggaat
     1561 gcggagttca atcgtatgct ccacgcctta tccccttggt agaatcgtct tgttcaagac
     1621 gcgtgaagta tacttccggt ttttgaggtc aacaataaat ttgcgtcagg acgcgagtgc
     1681 cacatcggcc ttggaaacag ccgtcctgcc gagatcccgg gcgtagcgcg agaggtcaat
     1741 ccgtgtatag cccatccctc gctgatcaag cccatcgatc gtgaccatct ccgttcccac
     1801 ctcgccatta tcgaaaccga gcgagagaac aaaatcaagg ggggtcgact gcggttttcc
     1861 cttgataccg aagtagagct ggttcccgct cttgacggaa agcaggtaga cgtaggaccc
     1921 tacttggcct ttggtattga aaacgggaat ctcggaaacg ctttcctctt gttcgaccga
     1981 ctcggggaca aagttcgcag aagcactcgt cccaccagat acaatctcgt cagacgggag
     2041 ggcgctcccg cccaagaggt cgtccaccga atccgtggcc gnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nccggtgtgt ccaagccacc caggaggtcg tcagacggga
     2221 gggcgctccc gcccaagagg tcgtccaccg aatccgtggc cggtgtgtcc aagccaccca
     2281 ggaggtcgtc agacgggagg gcgctcccgc ccaaaaggtc atccaccgaa tccgtggccg
     2341 gtgtgtccaa gccacccagg aggtcgtcag acgtcgaatc aggttcatcc atactcgcca
     2401 gcagttcgtc catcgagctt tccccggccc tgctcatcaa tcccatttct tcgggaggaa
     2461 cggaaccatc attcgaaact tgatcctcaa gcgatgtccc gaacgatgcc ggtccatcca
     2521 gaatatcatc aagttgttca tcaccgagga agcctgtatc catctcaaga ccggccggga
     2581 tgttgtttcc cgtcccgaga tcgagatcgg acagcccgcc ctcaaaaacc gcattggcct
     2641 ccgccagaag atcatcaatg ctgccggtgt ccgcagtcgc ctgtaccccg gccgggccgg
     2701 tcaactccga gagtcctgca tcggaaccgg acggttcctt gcccatgatc gcatccatcg
     2761 ctccgtccag tgccgcggac gagagccctt ccattgaatc cagatcatcg aggcggaggg
     2821 cattttcctg atcatcctcc cccgcgggaa tgatctgttc tgcaggcaaa ccgtccgtga
     2881 gtccgagggc ctggtcgacg tccagattgg caatctcttc gttctcccgc tccaggacgg
     2941 ccggatcaag aatgggaaca cttccgttga gagccagatc acgatccagc gatgcgatca
     3001 ggtcatcaac ctcctggctg acccgtccgg catcctcgac gttcagctcg gggagcacat
     3061 catcctcgtc gggtttcagc ccatccatcg ggacaggctc gtcaggcaga ctcatcgtct
     3121 cggtgatcga gcgcaggaca tcatcttcat cactcccggt atccagatca ttcagcagga
     3181 ggcggtcatc atcgtccggt tcaatcccgg acatcggcac cggttcagcg gcaatatcca
     3241 tcgaggaggt gatcgaaccg aggatgtcgt cctcatcatc cgaaaccggt gtcacacctg
     3301 tctcgagatc atccaaaagg agcctgtcat cttcatccgg ttcaatcccg gacatcggga
     3361 ccggttccgc cgcgatgtcc atcgacgatg taatcgagcc gagaatgtca tcctcgttac
     3421 ccgaaaccgg tgttacgcct gtctcaagat cgtccaaaag aagtctgtca tcctcgtcgg
     3481 gctcgacacc gtccatgggg acaatttcgt cggacaacgg catcgacgac tcgattgaat
     3541 ccaggagcgt gcttccgtca atctccggcg aatcaacgtc acccagtgtc aggcgttcct
     3601 catcatcacc ggccacagat ccatcattga acccggtccc ggaccgcatg ggggactccg
     3661 taaactcgtc agaagcaagc atggcggcag cgccggccac gagtgccgtc ccggccacag
     3721 ctggagccgg ccccttgccg tacccggaga ggacatcttc atcctcatct accacctgca
     3781 actcgtcact gaagtcgttt tcggcggccg gtaaaagcca ggcagcctca tcctcggccg
     3841 cattcccggc atcgcgttcc cagtccgtcc aggttgcatc ctcgcgctct tggtcgtccc
     3901 gcaggctgcg catatcctcg gtgctcagga ggtccacacc tggaatggta acctcgtcga
     3961 tgaccgtctg cccatcatcg gcaagatccg gccgaagccc ccacaacgcc cgcatccgct
     4021 ccacaagaag ttgccatgcc ctgcccctct cgtgggtggc gatgtagagc ctgaaataca
     4081 tgcaagacag gaacgagagc gccggtgcca gcatggcggc aaagatatgc gccagcgaga
     4141 tcgcggcaaa caccgccgag agggccgcca tcggatcctg gacccctgaa acgacattct
     4201 ggctccaggc aaggagttgc tgggtgacac tgcccatcac gccggtcatg ctcccgaagc
     4261 tgtccgtgac aaggagcgaa aggagatacc cgcccgcaat gatcgccacg ggaacagcaa
     4321 ccagaaccgc aaccgcgaga ccggccggta tcgaaacgaa gacatatctg aaaaagtttt
     4381 tcctgacgat gccgagaagc tgggcggatt ccctgaacaa cctgcggatg aaactgccct
     4441 ggagcggtgt caacgcgaaa agcacatgcg agggaaagac agccaacccg aaggcaatca
     4501 ggatcagaca ggcaacggca aaaatcgaaa cggccacaag cggaaccagg acaatggtgt
     4561 acagcagcga tccgatctgg ggaacggcaa ggaggagcgt ccca
//
LOCUS       DAOSHE010000128         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16889_length_4581_cov_4.550155, whole genome shotgun sequence.
ACCESSION   DAOSHE010000128 DAOSHE010000000
VERSION     DAOSHE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16889_length_4581_cov_4.550155"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLD82_04800"
     CDS             <1..319
                     /locus_tag="PLD82_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64737.1"
                     /translation="TALLACGLATLGILEFIARDQALLQDSGVIANIFAALARIALLA
                     GTFLAILSLAGYGAEALRQAGNPPRKTLLFGLALVLGTLLIILVLRASEIPSIESLPG
                     PWL"
     gene            333..1067
                     /locus_tag="PLD82_04805"
     CDS             333..1067
                     /locus_tag="PLD82_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG64738.1"
                     /translation="MRLAIISDIHGNLEALRSVLGRIDESRCDEIWCLGDIVGYGADP
                     SACIELVRERCSLVVAGNHDTTATRDQIPPGYNSLARQALLWTKARLSSDEVTWLRSL
                     PVTALRFSCLACHGSLRQRDAYVDSDREARINLELMQAQYPEASIFLCGHTHVKAWAA
                     RGTRWKSFSSGTAFDWPEQGPILVNPGSTGQPRDGNPRASWALLDLETRRCELKTASY
                     EIGLAQEKIVKGGLPIWLAERLDTGT"
     gene            1170..1310
                     /locus_tag="PLD82_04810"
     CDS             1170..1310
                     /locus_tag="PLD82_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64739.1"
                     /translation="MPTFKIDGAKFASLEELKSSLWEMYKDKMSKDEFEKYVKENVVE
                     TP"
     gene            complement(1548..2450)
                     /locus_tag="PLD82_04815"
     CDS             complement(1548..2450)
                     /locus_tag="PLD82_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG64740.1"
                     /translation="MEAMLDNGLDKVSSYIKESNRDEFSSGPLSIYLNEIDKIPLLTR
                     EQEVSLAKRMAGGDMKAKEQLIKSNLRFVVNIAKKYRNNGLPLIDLINEGNLGLIIAA
                     ERFDYTKGYHFISYAVWWIRQSILKALSEKARLIRLPLNRANELLQIEKIKREFSQKN
                     GDNPTAIEIAEILDMKKNDVKDILNASKQYVSIESPLGTSDRDGKLSDLIEDANGHEP
                     EQDLIMKDLKETIRKTLETLSENEREVIKYRFGLEGVRPMSLKELGKMFGLTKERIRQ
                     IEKKAIKRLKHPSRRRRLEDFVHG"
     gene            complement(2702..4057)
                     /locus_tag="PLD82_04820"
     CDS             complement(2702..4057)
                     /locus_tag="PLD82_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG64741.1"
                     /translation="MPPRILVVDDEQHIREGIGEFFRGEGYAVSLAEDGNQGLNSVRG
                     GDIDVVITDLRMPGMNGETLIEEALRINAGMPIIVLTAHGSVENAVECMRKGAYEYRT
                     KPVDLKELALLVQRALSVRALEKENENLHIQLQDRFGLTNIVGHSPEMERVFDQVRTV
                     APTKANVLITGESGTGKELIANAVHLLSERARHPFVKVHCSALAPGLLESELFGHEKG
                     AFTGANRLHRGRFELANQGTLFLDEIGEIDAAIQVKLLRVLQEKKFERVGGEETISVD
                     VRLVTATNRDLYRMVQEGTFREDLYYRLKVVHIHVPPIRERRSDIPLLVTSFLKEFCR
                     ENGTMEKKIDRRAMAALEQYRWGGNVREMKNLMENLVVMSKGPVIQYADLPEYIRDEK
                     QPDTITLPAGKKLEEYEKEIILANLAWVNGNKSRAAEVLGIGRKTLHRKLEEYGYSVS
                     E"
     gene            complement(4230..>4581)
                     /locus_tag="PLD82_04825"
     CDS             complement(4230..>4581)
                     /locus_tag="PLD82_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64742.1"
                     /translation="QEATKPGGRITISVRREEEMVAIAVEDTGSGIEKDRLGAIFEPY
                     HTSKEFGTGLGLTIVWRIVREHGGEIHVDSLPGEGSCFTVLLPAGKRPPGLLTAPEAG
                     GLSTERDGDLRGPS"
BASE COUNT          920 a         1372 c         1193 g         1096 t
ORIGIN      
        1 gactgccttg ttggcctgcg gtctggccac actgggcatc cttgagttca tcgcccggga
       61 ccaagccctg cttcaagaca gcggcgtcat cgccaacatt ttcgcggctc ttgcacgcat
      121 cgccctcctc gcaggaacct tcctcgcgat tctctccctg gcaggatatg gcgctgaagc
      181 cttgcgtcag gccggaaatc ctccccggaa aaccctcctc tttggcttgg cccttgtcct
      241 tggtaccctg ctcatcatcc tggtccttcg ggccagcgag attccctcga tcgaatctct
      301 gcccggcccc tggctctgag cggagaaggc atatgcgact ggccatcatc agcgatatcc
      361 acggaaacct tgaggccttg cgatccgtcc tgggccggat tgacgagtcc cgttgtgacg
      421 agatctggtg cctcggggat attgtcggat atggcgctga cccttccgcc tgcatcgagc
      481 tcgtccggga acggtgcagc ctggtggtcg ccgggaacca tgatactacg gcaacccgtg
      541 accaaattcc acccggctac aactccctgg cccgtcaggc cctgctctgg acaaaggcca
      601 ggctctcgtc cgacgaggtc acttggctgc gttccctccc ggtgaccgca ctgcggttct
      661 cctgcctggc ctgtcacggt tctcttcgac agcgggacgc ctatgtcgac tccgaccggg
      721 aagcccggat caacctcgaa ctgatgcagg cccagtatcc cgaagcatcc atcttcctgt
      781 gcggccatac ccacgtcaaa gcatgggcgg cacggggaac ccgctggaag agtttttcgt
      841 ccggaaccgc tttcgactgg ccggaacaag gccctatctt ggtcaatccc ggttcgaccg
      901 gccagccgag agatggaaac ccccgggcca gttgggccct gcttgacctc gaaacgcggc
      961 gctgcgagct gaaaacggca tcctatgaga tcggcctcgc ccaggaaaaa atcgtcaagg
     1021 gcggactgcc aatctggctc gcggagagac tggacaccgg gacttgacgc gttcgctcct
     1081 ggccagtctc ccgattttca aagattcttt ttgactgcca atccccacag tggtatatat
     1141 tgagtataga atcaggaagg tgaaacgcta tgcccacgtt taaaattgac ggtgccaagt
     1201 tcgccagcct tgaggagctg aagtcctctc tctgggagat gtacaaggac aaaatgtcca
     1261 aagacgagtt cgaaaagtat gtcaaggaga atgtcgtcga aactccgtga tgtccatgtg
     1321 cctgatggtc tgcttcgcag gcctttatat atatgccgtc gtccttcggg gcggcggttt
     1381 tttattcgcc cggtactcat ccataggatc cggcaacctt ctgacacaaa agatcttgcg
     1441 gaatccgggc gtacagacaa caacccgatc agggacgtcc aaagcaagag gccgcccttc
     1501 cgggacggcc tcttgtcgat catacttgaa ttctgctccg tcaaacctca gccgtgcacg
     1561 aaatcctcga ggcggcgccg gcgggagggg tgcttgaggc gcttgatggc cttcttctcg
     1621 atctggcgga tgcgctcctt tgtcagaccg aacatcttgc caagctcctt gagcgacatc
     1681 gggcgaaccc cttccaagcc aaagcgatac ttgatgacct cgcgctcatt ctccgacagc
     1741 gtctccagcg tcttgcggat ggtctccttg aggtccttca tgatcagatc ctgctccggc
     1801 tcatgtccgt tggcatcctc gattagatcc gagagctttc cgtcacggtc gcttgtcccg
     1861 aggggggatt cgatcgaaac gtactgcttc gaggcattga ggatatcctt gacatcgttt
     1921 ttcttcatgt cgaggatctc ggcgatctcg atggccgtcg gattgtcccc gtttttctgg
     1981 gagaactcac gcttgatctt ttcgatctga aggagttcat tcgccctgtt gagcggaagt
     2041 cggatcaggc gagccttctc cgaaagtgcc ttgagaattg actggcgaat ccaccacaca
     2101 gcgtacgaaa tgaagtggta acccttcgta tagtcaaagc gctctgccgc gatgatcaaa
     2161 cccaggttcc cttcgttgat caggtcgatc aaggggaggc cgttgttgcg atactttttg
     2221 gcgatattga ccacaaagcg caggtttgac ttgataagct gctccttggc cttcatgtca
     2281 ccgccagcca tcctcttggc aaggctgact tcctgttcgc gggtcaaaag cgggatcttg
     2341 tcaatctcgt tgaggtagat cgagagaggc ccggaggaaa attcatccct gttgctttct
     2401 ttgatgtatg agctgacttt atcgagtccg ttgtccagca tggcttccat gatctctctc
     2461 cttacgatgt tgttgccttt tgtctgcttt tccgtatctt ttatgcccac tagtgattgc
     2521 aggttttatg ccattatcca atcggcaaca aaaaaaataa atattttatt actgtttaca
     2581 atacaatcag ttacagatta aagtttttct tcggagttgt tcgtttttcc ctaacgacac
     2641 agtcctcagc ttcttgctgt gtcaatttga cgcaccatgt ctcagacgcc tgaattctgt
     2701 gtcactctga cacagaatac ccgtattcct cgagtttcct gtggagcgtt ttgcgcccaa
     2761 tcccgagaac ctcggcggca cggctcttgt tcccgttgac ccaagccagg tttgccaaaa
     2821 taatctcttt ttcgtactct tcgagtttct tgccggccgg gagggtgatt gtatccggct
     2881 gcttctcatc acggatgtat tcaggcagat cggcatactg gatgaccggc cccttgctca
     2941 tgacgaccag attctccatc aggttcttca tctcgcggac gttcccaccc cagcggtact
     3001 gctccagcgc cgccatggcc ctccggtcga tttttttctc catggtgcca ttctcccggc
     3061 aaaactcttt gagaaaggag gtcacgagga gggggatatc actgcggcgc tcccggatgg
     3121 gcgggacatg gatgtggacg accttgagac ggtagtacaa atcctcgcga aaagtccctt
     3181 cctggaccat ccggtagaga tcccggttgg tcgcggtcac cagacggaca tcgaccgaga
     3241 tggtctcctc cccgccaaca cgctcaaact ttttttcctg gagcaccctc aggagtttga
     3301 cctggatggc tgcgtcgatt tcgccgatct catccagaaa cagggtcccc tgattggcca
     3361 gctcgaaccg tccacgatgc aggcggttgg ctccggtaaa agcccccttt tcgtgtccga
     3421 acaactcgct ctcaagcaaa ccgggcgcaa gggctgaaca atggaccttg acaaagggat
     3481 gtcgcgcccg ctcggaaaga aggtggaccg cgttggcgat caattccttg cccgttccgg
     3541 attcgccggt aatcagaaca ttggccttgg tcggtgcgac ggtccggacc tgatcaaaaa
     3601 cccgctccat ctccggacta tgcccgacga tattggtcag accgaatcgg tcctggagct
     3661 gaatatggag attctcgttt tctttttcga gcgcacgcac cgagagggcg cgctggacga
     3721 gaagggccag ttccttgaga tcgaccggct tggtccgata ctcgtaggcc cccttgcgca
     3781 tgcattcgac ggcattttca accgacccat gggcggtcag gacgataatg ggcatgccgg
     3841 cgttgatcct gagggcctct tcaatcagag tctcaccgtt catgccgggc atacgaagat
     3901 ccgtgatgac gacatcgata tcaccacccc ggaccgagtt caacccctga tttccgtctt
     3961 cagccaatga aaccgcatag ccctcccccc gaaagaactc cccgatcccc tcgcggatat
     4021 gctgttcatc gtccacaacc aggattcgtg gcggcatacg gttttcctca ataaagaatg
     4081 cttttctctc aattttgact tgtgtcattt taacacaacg cccaggacct gacaagacag
     4141 tttgacacag tttttggatt ttttgaggaa caccctcgcg cccggactgg gtcatttcgt
     4201 ccgaaagatg acgaccgccc gtcagcggat caagacgggc cccggagatc cccgtcccgc
     4261 tccgtggaga gaccacctgc ttcgggagcc gtcaacaagc ctggaggcct ctttcccgcc
     4321 gggaggagga ccgtaaaaca cgagccttct ccggggaggc tatcgacatg gatctccccg
     4381 ccatgttcgc gaacgatccg ccagacaatg gtcaacccca gcccggtgcc gaactccttg
     4441 ctggtgtgat acggctcaaa tatcgccccg agccggtcct tctcgatccc gctcccggta
     4501 tcctcgacgg caatcgcgac catctcttct tcacgacgga ccgagatggt gatcctcccg
     4561 ccgggtttgg tcgcctcttg g
//
LOCUS       DAOSHE010000129         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_16928_length_4576_cov_4.246406, whole genome shotgun sequence.
ACCESSION   DAOSHE010000129 DAOSHE010000000
VERSION     DAOSHE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16928_length_4576_cov_4.246406"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..689
                     /locus_tag="PLD82_04830"
     CDS             78..689
                     /locus_tag="PLD82_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOG64743.1"
                     /translation="MQGKLQELTEKLYAEGVAKANDEAGRITAEARQKADSILAAARA
                     EADSIKSQAEREVADLKRNVESELKMAAQQAVSALKDRISRLVTAGLADAPVGQAMSD
                     VEFVKQLITAIVGNAGSDLASLKLELPGAMEGKVQDFLKTGSLAKLGKGLEVVFDRTL
                     SGGFRVGPADQGFVVSFTDEGFAEFFREYLRPRTRQFLYGDEG"
     gene            696..1538
                     /locus_tag="PLD82_04835"
     CDS             696..1538
                     /locus_tag="PLD82_04835"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOG64744.1"
                     /translation="MARAYYCLVAGFPDFALDEAKLPLTTASLRADLEEQLAPEDRAY
                     LAMLFWPVDCRNLLSVLTGKPEHDASGNFARDVLEEEVRTPVLLPLYMREFLDLYRAG
                     LSAGVHPENVLADLMYREKAGCSNAFIRGWSAFDRDLRNIVAALGCRRTGAEPEREIV
                     GEGLVAEAVRRASGSDFGLARELVWLEPLIRSWGQTLLDRELAIDRIKMDVLDELTVS
                     SGFSIDKVLAFVLRLGIAERWLALDAATGREMLRRILTDMESGLVFPEEFSIRGGSKH
                     GDNR"
     gene            1522..3288
                     /locus_tag="PLD82_04840"
     CDS             1522..3288
                     /locus_tag="PLD82_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOG64745.1"
                     /translation="MATTGKVAGIIANLVMVEVDGPVSQNEICYIDVGGTKLMSETIK
                     VVGNTAYVQVFESTRRMRVGNPVEFAGHMLEVTLGPGILSRNYDGLQNDLDKMKDVFL
                     QRGEYTYPLDESAKWDFVPLAKVGEGVSAGSWLGEVKENWIGHKIMVPFSFEGKYTVA
                     SIVSAGSYGINDTIAVLKDEKGGEHTVTMVQKWPVKLAVRAYKDKKRPFKIMETGTRT
                     IDTLNPIAEGGTGFIPGPFGCGKTVLQHSLAKFAEADMIIVAACGERANEVVEIFTEF
                     PELDDPRTGRKLMERTTIICNTSNMPVAAREASVYTAMTIAEYYRSMGLRVLLLADST
                     SRWAQALREMSNRLEELPGPDAFPMDLSAIVANFYARAGYVILNNDQPGSITFIGTVS
                     PAGGNLKEPVTESTKKAARCFYALAQPRADSKRYPAIDPIDSYSKYLEYDEVQQYLNE
                     KISPEWVSNVYLAKDILLRGREAFEQINILGDDGVPIEYHERYWKSEIIDFVILQQDG
                     FDKIDQSTPIERQRFMLDRVLGICKGAFKFESFEEVNPYFKRIINTFKQMNYSEYNSD
                     KFKTLDADLGKILAEREVVHAN"
     gene            3278..>4576
                     /locus_tag="PLD82_04845"
     CDS             3278..>4576
                     /locus_tag="PLD82_04845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOG64746.1"
                     /translation="MQTKAGQKIYSKITQITKATCSLQATGVGYEEMAMVHGRLAQVV
                     KIVGDRVTLQIFSGTEGIPTNAEVVFLGHPPTVKVSEELAGRFFDAYGKPIDGKPEIN
                     GEEREIGGPSVNPVRRKQPSELIASGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAM
                     VAMRAKADRIILGGMGLSNDDYLFFKSVFENAGALDRIIAFVNTTENPPVERLLVPQT
                     ALTAAEYFAVDRNEKVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLASIY
                     EKAVQFPSGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTRNDHPQVMNAAVRLYADAANARTKMENGFDLSDYDERTLRFAKE
                     YSNSLLAIDVNVDVDTMLDTGWKLFSRHFTQAEVGIKQELM"
BASE COUNT          949 a         1311 c         1348 g          968 t
ORIGIN      
        1 cgcaaaaaaa gcgtttcggg tattgcattg acgcgcctga tacggtaacg tataggccac
       61 tatccgtgga ggttgctatg cagggcaaac tgcaggagtt gacagaaaag ctgtatgccg
      121 agggtgtcgc caaggccaat gacgaggccg gtcgcatcac ggcggaagcc agacaaaagg
      181 cagattcgat tctcgctgcg gcacgtgccg aggcggactc gatcaagtca caggcggagc
      241 gcgaggtggc agacctcaaa cgcaacgtcg aatccgaact caagatggcg gcacagcagg
      301 ccgtcagtgc actcaaggac cggatctccc gtctcgtgac ggccggtctc gcggacgcgc
      361 ctgtcggaca ggccatgtcg gatgttgagt ttgtcaaaca gctcattacg gccatcgtcg
      421 ggaatgccgg ttccgatctg gcttccctca agctcgaatt gcctggggcg atggagggca
      481 aggtccagga tttcctcaag accgggtctc tcgccaagct ggggaagggt ctggaagttg
      541 tctttgaccg gaccttgtcg ggcggattcc gtgtcggccc ggccgatcag ggctttgttg
      601 tttcgttcac ggatgagggc ttcgccgagt ttttccggga atacctcagg ccccgcaccc
      661 gccagtttct ctacggcgat gaggggtgag gtcagatggc acgtgcctac tactgtctgg
      721 tagccggctt tcccgatttc gcactggacg aggccaagtt gccgttgacg acggcatcgc
      781 ttcgggcgga tcttgaggaa cagctggccc ctgaggacag ggcgtatctg gccatgctct
      841 tctggccggt cgattgccgc aatctcttgt cggtcctgac cggaaagccg gagcatgatg
      901 ccagcgggaa ttttgcccgt gatgtcctcg aagaggaagt gcgcaccccg gttctcctgc
      961 ccctctatat gcgcgagttt cttgatctgt atcgtgcggg gctgtctgca ggtgtccacc
     1021 ccgaaaacgt tctggccgat ctcatgtatc gcgagaaggc cgggtgttcc aacgccttta
     1081 tccgtggctg gtcggcgttt gaccgggatc tgcgtaatat cgtcgcggct ctcgggtgcc
     1141 ggcggaccgg tgcggaaccg gagcgggaga tcgtcggtga agggctggtt gccgaagctg
     1201 tccggcgcgc cagcggctcc gatttcggtc ttgcccgtga gctggtctgg cttgagcccc
     1261 tgatccgttc ttgggggcag accctcctgg accgcgagct ggccattgac cgcatcaaga
     1321 tggatgtact ggacgagttg accgtttcaa gcggcttctc gattgacaag gtgctggcct
     1381 ttgtcctgcg tttgggtatt gccgaacgct ggctggcact ggatgccgcg acgggccggg
     1441 agatgctgcg gcgcatcctc accgatatgg agtccggcct ggttttccct gaagagttca
     1501 gcatacgcgg aggaagcaag catggcgaca acaggtaagg tggcgggtat catcgccaac
     1561 ctcgtgatgg tcgaagtgga cgggccggtc tcgcaaaacg agatctgcta cattgatgtg
     1621 ggcggcacca agctcatgtc cgagacgatc aaggttgtgg gcaacacggc ctatgttcag
     1681 gtcttcgaga gtacccggcg gatgcgcgtc gggaatccgg tcgaattcgc agggcacatg
     1741 cttgaagtga ccctcggtcc cggtattctt tccagaaact atgacggtct gcaaaacgac
     1801 cttgacaaga tgaaggatgt gtttctgcag cggggcgagt acacctatcc gctggacgag
     1861 agcgcaaagt gggacttcgt tcccctggcc aaggtcgggg agggggtttc tgccggatcc
     1921 tggttgggcg aggtcaagga gaactggatc ggtcacaaga tcatggtgcc attttccttc
     1981 gaggggaagt acacggtcgc ctcgatagtt agcgccggga gttacggtat caacgatacg
     2041 atagctgtcc tcaaggatga aaagggcggg gaacacacag tcaccatggt gcaaaagtgg
     2101 cccgtcaagc tggctgtccg tgcctacaag gacaagaagc gccccttcaa gatcatggag
     2161 accgggacgc gcaccatcga cacgctcaac ccgattgccg agggtgggac gggtttcatc
     2221 cccggtcctt ttggttgcgg caagaccgtg ttgcagcact ccctggcgaa atttgccgaa
     2281 gccgacatga tcatcgtggc agcctgcggc gaacgcgcca acgaggtcgt cgaaatcttt
     2341 accgagttcc ccgaactgga tgacccccgg accggccgca agctgatgga gcgtaccacg
     2401 atcatctgca acacctccaa tatgcctgtt gccgcgcgtg aagcctcggt ctatacggcc
     2461 atgaccattg ccgagtacta ccgctcgatg ggcttgcggg tccttctcct ggctgactcc
     2521 acctcccgct gggcacaggc cctccgcgag atgtccaacc gtcttgagga acttcccggt
     2581 ccggatgcct tcccgatgga cctttcggcg attgtagcca acttttacgc ccgggcggga
     2641 tacgtcattc tcaacaatga tcagcccggt tcgatcacct tcatcggaac tgtgtcccct
     2701 gccggtggaa acctcaagga gccggtcacc gagtcgacca aaaaggccgc ccgctgcttc
     2761 tatgccttgg cccagccccg cgcggattcc aagcgctatc cggccatcga tccgatcgat
     2821 agctactcca agtatctcga atacgacgag gtccagcagt acctcaacga gaagatctcg
     2881 ccggaatggg tctccaatgt ctacctggcc aaggatattc tcctgcgcgg tcgggaggct
     2941 ttcgagcaga tcaacatcct cggagacgat ggtgttccga tcgagtacca cgaacgctac
     3001 tggaagtcgg agatcatcga ctttgtcatc ctgcaacagg acgggttcga caagatcgac
     3061 cagtctactc cgatcgagcg gcagcgcttc atgctcgacc gtgttctcgg gatttgcaaa
     3121 ggcgcgttca aattcgagag cttcgaagag gtcaacccgt atttcaaacg catcatcaat
     3181 acattcaagc agatgaatta ttccgaatac aactcagaca agttcaagac actcgacgct
     3241 gatcttggaa agatcctcgc cgagcgggag gttgttcatg caaactaagg ccgggcagaa
     3301 gatatattca aaaatcaccc agatcaccaa ggccacatgc agtctgcagg ctaccggcgt
     3361 gggatacgag gaaatggcca tggtgcatgg ccggcttgcc caggtcgtca agatcgtcgg
     3421 tgaccgtgtc acgctacaga tcttttcagg aacagagggg attcccacaa acgccgaggt
     3481 tgttttcctg ggtcatccgc ccacggtcaa ggtcagcgag gaactggcgg gccgcttctt
     3541 cgatgcctac ggcaagccga tcgacgggaa gcccgagatc aatggtgaag agcgcgagat
     3601 cggtgggcct tcggtcaacc cggtccgccg caagcagcct tccgagttga ttgcgagcgg
     3661 tatcgccggt attgacctca acaacacgct tgtgaccggg cagaagattc cgttttttgc
     3721 cgaccccgac cagccctata accaggtcat ggccatggtg gccatgcgcg ccaaggctga
     3781 ccggatcatt ctgggaggca tgggtctctc caatgacgac tacctcttct tcaagagcgt
     3841 ctttgaaaat gccggagcgc tggatcgcat catcgccttt gtcaacacga ccgagaatcc
     3901 tccggtcgaa cggctcctgg ttccacagac agcgctgacg gccgccgagt atttcgccgt
     3961 ggaccgcaac gaaaaggttc ttgtccttct gaccgacatg acgctctatt gcgatgcact
     4021 ctccattgtt tccaaccgca tggaccagat tccttccaag gacagcatgc cgggttctct
     4081 ctacagtgat ctggccagca tttacgaaaa ggccgtccag ttcccgtccg gtggctcgat
     4141 caccattatc gcggtcacca ccctctccgg tggcgatatt acccacgcta tcccggacaa
     4201 taccgggtat atcaccgagg gccagctctt cctcaggcgc gatacggata tcgggaaggt
     4261 cattgttgac cccttccgct cactctctcg tctcaagcag ctcgtgatcg ggaagaaaac
     4321 ccggaacgat cacccccagg tgatgaatgc tgcggtccgt ctctacgccg atgcggccaa
     4381 tgcccgtacc aagatggaaa acggttttga cctttcggac tatgacgagc gtacgctccg
     4441 gtttgccaag gaatactcca actcgctctt ggcgatcgac gtgaatgtcg acgtcgatac
     4501 gatgctcgat accggttgga aactcttttc ccggcacttc actcaggccg aggttggtat
     4561 caagcaggag ctgatg
//
LOCUS       DAOSHE010000130         4561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17006_length_4561_cov_3.947182, whole genome shotgun sequence.
ACCESSION   DAOSHE010000130 DAOSHE010000000
VERSION     DAOSHE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17006_length_4561_cov_3.947182"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLD82_04850"
     CDS             <1..385
                     /locus_tag="PLD82_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOG64747.1"
                     /translation="HTPEIAQELALAASFATPVIFTPHLLPIERGILSTIYFKSAKTA
                     EECRAHLAHFYEGEPFVRVLDNGLPEIAHVAHTNLCVCTVVPSGIPGTLIAVSTIDNL
                     VKGAAGQAIQNMNVRFGFPEETALV"
     gene            398..1630
                     /gene="argJ"
                     /locus_tag="PLD82_04855"
     CDS             398..1630
                     /gene="argJ"
                     /locus_tag="PLD82_04855"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOG64748.1"
                     /translation="MAVKKIFESVKLHHGEGGVTAPVGFVANGISCGIKTNGAKDLCI
                     IFSRDACNAAGIFTSNRIKGAPLKVSKTNLGNKVHAIVANSGNANACTGREGVQNVRE
                     IGKQIGDEFEIPSNSILYASTGVIGVPLPMESINYGIAKLKRKLIAENNVRNAADAIM
                     MTDTKRKMAKVTCIIGGKLITIGAIAKGSGMINPNMATVLTLITTDANIAPKMMQRAL
                     RSAAKHTFNKLTIDGQMSPNDCVLMMANGRQNNQIINRTGVNYSIFEHALTMLCDCIA
                     MEIINDGEGVTKVITLTVKGAATKAEAELAAREIANSVLFKTAMYGQSANWGNIIQAI
                     GATEVAINPDKINVFMNGVQVCENSMFNGFKPADGRTILNKRNIQVTVDLGVGKASDY
                     IITTDLSYDYVKINSQYI"
     gene            1651..2517
                     /gene="argB"
                     /locus_tag="PLD82_04860"
     CDS             1651..2517
                     /gene="argB"
                     /locus_tag="PLD82_04860"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG64749.1"
                     /translation="MEAKIKRAEILIEALPYIRDFSGKPFVVKYGGSAMLNDELREKV
                     ARDLVLLKLVGINLVVVHGGGKHINEMLGKLNIKGEFRNGLRVTDEETMRVVEMVLSG
                     RSNKDIVTLINKHGGNAVGLSGKDGPLLHAVPYVDSDGGTYGQVGVVQSVSTPLIDRM
                     LDGDFIPVISPVAFGQDGKSYNVNADMVASRVAQSIHASKLIYLTDVPGVLRDPKDPA
                     SLVSTLTIQESRALITEGVISGGMLPKVNSIIECLENGVEKVHIVNGMTPHALLLEVF
                     TDGGIGTEFRAV"
     gene            2576..3514
                     /gene="argF"
                     /locus_tag="PLD82_04865"
     CDS             2576..3514
                     /gene="argF"
                     /locus_tag="PLD82_04865"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG64750.1"
                     /translation="MKSFRTKDLVSMESLTTADIIQIFETTARQKKERAKKGTNKPVL
                     QGKTLGMIFEKASLRTRISFEVGMLHLGGRAIYLDQTNFKLGERESVYDVAKNMERFM
                     DILMLRTFSHQAILDMARHASIPVINGLSDDEHPCQALADLYTIMEHRGELKGRKLVF
                     VGDGNNVATSLAYAAALMGMHFVCASPEGYTLKQEIVDKARSMAVFGATVEATTDVRE
                     AVKNADCLYTDVWASMGQEKEKEIRAAAFREYQINESLLALARPDCLIEHCLPAHRGE
                     EITDGVIDGPNSVVFDEAENRMHVQKAVMTLLLGRG"
     gene            complement(3650..>4561)
                     /locus_tag="PLD82_04870"
     CDS             complement(3650..>4561)
                     /locus_tag="PLD82_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64751.1"
                     /translation="QGALNRYSITPGQTMAFHTKIKTGRVQATALLYQTNLVFIVDNM
                     DKNIELDFPTRPVIFILPDSDSSISIQNKTIRKITSRRSIAYVPTSTKDLDFSWHTRR
                     GLSNILEKEYTIRLEKGNEPHTVDINALKSYMYSGLGLGYGLGFYVNLHPENSGSITF
                     SALGIFLEKPLEENTGLWGSFTINSYNNNTSALEEGTLFIFDIGLYHGIRLYNDNFLK
                     LRAGLGILAKATIDQDDSSFPDGATPRPNRDSTVFEFGIGWWYRSFGFHAGRSGSVLF
                     ELWTVIPKEMYGGALAPMCGLRLIFGF"
BASE COUNT         1114 a         1168 c         1221 g         1058 t
ORIGIN      
        1 gcataccccc gagattgccc aggaacttgc actggcagcc agctttgcaa cgccggtgat
       61 ctttacgccc catcttttgc caattgaacg gggcattctt tcaacgatct atttcaagag
      121 tgcgaagact gccgaggagt gtcgtgcgca tcttgcacat ttttacgagg gggaaccttt
      181 tgtccgggtt ctggataacg ggcttcccga gattgcacac gtggcacaca cgaatctctg
      241 tgtgtgtacg gttgtcccct cggggatacc gggaaccctg atcgcggtca gtaccatcga
      301 caatcttgtc aagggcgcgg ccggtcaagc catacaaaat atgaatgtgc gcttcgggtt
      361 tcccgaagaa acagcacttg tctgaagagg ggaatgcatg gcagtcaaaa agattttcga
      421 gagtgtcaaa ctccatcacg gggagggcgg agtcacggct ccggtcggtt ttgtggccaa
      481 cggcatctcc tgcgggatca agaccaatgg tgccaaggat ctctgtatca tcttttcacg
      541 tgacgcctgc aatgctgcgg gcatctttac cagtaaccgc atcaaaggcg ctccgctcaa
      601 agtctcgaaa acaaatctcg gcaacaaggt ccatgcgatt gtggccaaca gcgggaatgc
      661 caacgcctgt accggtcgtg agggtgttca aaatgtcagg gagatcggga agcagatcgg
      721 ggacgaattc gagatcccgt ccaattcgat cctctacgca tcgaccggag tgatcggcgt
      781 tccgcttccg atggaatcca tcaattacgg tattgcaaaa ctcaaacgaa aactgattgc
      841 cgaaaacaac gtccggaatg ctgccgatgc gatcatgatg accgatacca agcgcaagat
      901 ggccaaagtg acctgcatca tcggcgggaa actgatcacg atcggagcga ttgccaaggg
      961 ttccgggatg atcaatccca acatggctac agtcctgaca ctgatcacga cggatgccaa
     1021 tattgccccc aagatgatgc agcgggccct gcgctctgcg gccaagcata ccttcaacaa
     1081 attgacgatt gacgggcaga tgagtcccaa tgactgtgtc ctgatgatgg ctaatgggag
     1141 acagaacaac cagatcatca acaggaccgg agtgaattat tcgatcttcg aacacgccct
     1201 gaccatgctc tgcgattgca tcgccatgga gatcatcaac gatggcgagg gtgtgaccaa
     1261 ggtgatcacg ctcacggtca agggtgccgc aacaaaggct gaggccgaac tcgcagccag
     1321 ggagattgcc aactccgtac tcttcaagac agccatgtac ggccagtccg ccaattgggg
     1381 caacatcatc caggccatcg gggcgaccga agttgcgatc aaccccgaca agatcaatgt
     1441 tttcatgaac ggtgtccagg tttgcgagaa cagcatgttc aacggattca agcctgccga
     1501 tggcaggaca attttaaata aacgcaatat tcaggttact gttgacctcg gtgtcggcaa
     1561 ggccagtgat tacatcatca cgactgacct ctcctacgat tacgtgaaaa tcaattccca
     1621 gtatatctga agcgtttcgg gaccctcgct atggaagcca agatcaagag ggcggagatt
     1681 ctcattgagg ctctgcccta tatccgagat ttttcaggca agccctttgt cgtcaaatac
     1741 ggcgggagtg ccatgctcaa tgacgaactc cgggagaagg tcgcccgtga tctggtcctc
     1801 ctcaaactgg tgggcatcaa tctcgtcgtg gtgcacggtg gcggaaagca catcaacgag
     1861 atgctcggaa agctcaacat caagggcgag ttccgcaatg gtctgcgggt gacggacgag
     1921 gaaacgatgc gtgttgtcga gatggtcctc tcgggccgtt ccaacaagga catcgtgacc
     1981 ctgatcaaca agcacggcgg gaatgcggtc gggctttcag gaaaggacgg tcctctcctg
     2041 catgccgttc cctatgtcga ttcagacggc ggaacctatg gccaggtcgg tgttgtccag
     2101 tcggtttcga cccccctgat tgaccggatg ctggatgggg acttcatccc ggttatctct
     2161 ccggtcgcct tcggccagga cggaaagagc tataatgtca atgccgacat ggttgccagc
     2221 cgggtggcgc agtccatcca tgccagcaag ctgatctatc tgaccgatgt cccgggtgtt
     2281 ctccgtgatc caaaggatcc cgcgagtctg gtctcaaccc tgaccatcca ggaatcccgt
     2341 gcgttgatca cggagggtgt catctcgggc gggatgcttc ccaaggtcaa ctcgatcatt
     2401 gagtgtctcg aaaacggagt tgaaaaggta cacattgtca acggcatgac cccccatgcg
     2461 cttcttcttg aagtgtttac ggatggcgga atcgggacgg agttccgggc tgtttgatct
     2521 tgaatatccg gttttttaca ggactgtgct ctgaccggca atgaggagtt ttatgatgaa
     2581 aagtttcagg acaaaagatc tggtctccat ggagtcgctc acgacggccg atatcatcca
     2641 gatcttcgaa acaacagcac gtcaaaaaaa agaacgtgcc aaaaaaggta cgaacaaacc
     2701 ggtcctgcag gggaagaccc tcgggatgat ttttgaaaag gcctctctcc ggaccagaat
     2761 ctcgttcgag gtcggcatgc tccatcttgg tggacgggcg atctaccttg accagaccaa
     2821 tttcaagctc ggtgaacgtg aatccgtgta cgatgtcgcc aaaaacatgg agcgcttcat
     2881 ggacatcctc atgctgcgca ccttctcgca ccaggccatt ctcgacatgg cccgccatgc
     2941 aagcattccg gtcatcaacg ggctctccga tgacgaacat ccctgtcagg ctctggccga
     3001 cctctacaca atcatggagc accgtggcga actcaagggc aggaagctgg tctttgtcgg
     3061 tgacgggaac aatgtcgcca cctcattggc ctatgcagca gctctgatgg ggatgcattt
     3121 tgtctgtgct tcgcccgagg gctacacgct caagcaggag atagtcgaca aggcccggtc
     3181 catggctgtc ttcggtgcga ctgtcgaagc gacaaccgat gtccgtgaag ccgtcaaaaa
     3241 tgccgattgc ctctataccg atgtatgggc cagtatgggc caggaaaaag aaaaagagat
     3301 ccgtgcggcc gctttcaggg agtaccagat caacgagagt cttttggcac tggccaggcc
     3361 ggactgtctg atcgagcatt gtctgcctgc ccatcgcggg gaggagatca cggacggtgt
     3421 catcgacgga ccgaactccg tggtttttga cgaggccgaa aacaggatgc atgtccaaaa
     3481 agcggtcatg accctcctgt tgggccgggg gtaaaacagg catttgcttc gcatgagtga
     3541 ctgtttttga tggtggtgtg catgcgatgc atcaggcatg cacgggcggg gtatcttgtt
     3601 tcagacaaga cgatacgttc atttgcagtt caggaattcc agtacactgt cagaatccga
     3661 atatcaacct gagtccacac atgggtgcga gggctccacc gtacatctct tttggaatga
     3721 cggtccagag ttcgaataga accgagcctg agcgcccggc atgaaatcca aaactgcgat
     3781 accaccagcc gattccaaat tcgaagacag tcgaatcacg gtttgggcgc ggcgttgcac
     3841 catcaggaaa tgatgaatca tcctgatcga ttgtggcctt ggccagtatc cccaatccgg
     3901 ctcgcaattt caaaaaattg tcattataca acctgatacc gtgatagaga ccgatatcaa
     3961 atatgaaaag cgttccctct tcaagagcgg aagtgttgtt gttgtacgaa ttaatcgtga
     4021 aacttcccca cagtccggta ttttcttcaa gtggtttttc aagaaagatc cccagagccg
     4081 agaatgtgat tgatccactg ttttcggggt gcaagttcac atagaatccg agtccatatc
     4141 ctagccccaa tcccgaatac atgtagctct tcaatgcatt gatatcgact gtatgtggtt
     4201 cgtttccttt ctcgagccgg atggtatatt ctttttccaa tatgttcgag agaccccgtc
     4261 ttgtgtgcca actgaagtcc agatccttgg tgctggttgg cacatatgcg atactgcgtc
     4321 tgcttgtgat ttttcgaatc gttttgtttt ggatggaaat cgaagaatcg gagtccggca
     4381 gtatgaaaat gactggtcgg gttggaaagt ccaattcgat attcttgtcc atattatcaa
     4441 cgataaagac caggtttgtt tgatagagca gagctgtcgc ctgaactcta cccgtcttga
     4501 tttttgtgtg aaaggccatc gtttgtcccg gggtaatgga atatctgttc aaagcacctt
     4561 g
//
LOCUS       DAOSHE010000131         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17067_length_4550_cov_3.827364, whole genome shotgun sequence.
ACCESSION   DAOSHE010000131 DAOSHE010000000
VERSION     DAOSHE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17067_length_4550_cov_3.827364"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2407)
                     /locus_tag="PLD82_04875"
     CDS             complement(<1..2407)
                     /locus_tag="PLD82_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOG64752.1"
                     /translation="MRVWSRIACAAITATALAACSIAPEVAGVDDRTSQDDAVKARTV
                     TSYTETFDSCTATGTSYVTGSFAGVGGWSWAYTKTAWGGVTLTGKTAVLGKGQSPTSA
                     LSVTVNNGVGTLSFKYKKAYSTATSLLVKVNGTTIATLVTSGTTVLTAGPYTVNRSGS
                     VTIQLIQSSTSAGQVAVDDISWTSYDGGTSSSSSVSSSSSSSSSSSSSVSSSVSSSSS
                     APSGWVSVAKSLTSPNYPSNYPASQDVTSTLTQAGASKVRVHFSAFNTENNYDKVYIM
                     NGLGTVLATYTGNKGAFTSIEVAGDTVKIRFTSDSSVAAAGWKIDALEYTTGGTSSSS
                     SSVSSSSSSSSSSSQSATVKNWTVMVYLDADNNLEAAGLEDMNEMEKGLANAGVSISD
                     NINVIVLLDRATGSSTADGYTSAKTESTGSDWSDTRLYRVRPDSSSMTYFNSERLDDN
                     GAGLGHIANCGEKNMGDPATLSWFVNYGKTYFPANNYMVVLWNHGGGARDWTPAERPA
                     KAVCWDDASGDDCLYLDELQQALAQRFTSSSKLGILGFDACLMSTVETAYEFRNLASY
                     MVGSMQTEQGDGWDYEYIFGNLGSASITPVNFATLVVDAYKVQIQANPVGDGETLAAI
                     DLSQISALKTAVDSMAVAMYTENKKSSIESTRNSAVKYYDNSDTYSRQEYPYFDLGSL
                     CSKIAASSTCSTALKTAANNVLTALGNAVVKAYGETATVASGVSGYGTSYYWGTGSSV
                     KRGLSIFFSYSSSDYAAQDWYTSRDQRNGTSSQVYGNMDFCTFNSDGSVTSWLELMEA
                     WYDP"
     gene            2594..4030
                     /gene="gatB"
                     /locus_tag="PLD82_04880"
     CDS             2594..4030
                     /gene="gatB"
                     /locus_tag="PLD82_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG64753.1"
                     /translation="MEFEAVIGLEVHAQLNTQTKIFCSCANRFGAEPNTQTCPVCQGH
                     PGVLPVLNGEALTKAIKAGLALDGSIAHYSRFDRKNYFYPDLPKAYQISQYEFPIVVG
                     GGVTVVREDGSEHTFPLTRIHMEEDAGKLVHPGDDAAHSLVDLNRAGTPLIEIVSEPE
                     LHTPEDAAMYLTELRAILRYIDVSDCNMEEGSLRCDANVSIRPKGTTTLGTKVEIKNL
                     NSIKGVAKAIEYEIRRQTEALLSGTPIIQETRLFDADREITLSMRSKEEAHDYRYFPD
                     PDLVPVLVPEERIRTLKDTLPELPRARKTRFITEYCLTPKDVGTLCATRELADWFETA
                     ARAGKNPKKIANWIQAEVLRILNNSGQGITAFPVSPENTAALMDLVEDGVISGKIAKD
                     VFEKMISTGKSAESIIEADGLRQVSDTGAIEQVIDEVLAANPAQVEQFKSGKTAVKGF
                     LVGQIMKKSGGKVNPAMVNQILEQKLQS"
     gene            complement(4346..>4550)
                     /locus_tag="PLD82_04885"
     CDS             complement(4346..>4550)
                     /locus_tag="PLD82_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64754.1"
                     /translation="FSDDAKAFARSRQMSLIERDEIRTLLGMGFVPGLTAGAPEKAGG
                     KPVVVGYEKAATNARLRQSAGVA"
BASE COUNT         1073 a         1354 c         1166 g          957 t
ORIGIN      
        1 acggatcgta ccaggcttcc atcaattcca gccagcttgt caccgaacca tcgctgttga
       61 atgtgcagaa gtccatgttt ccatatacct gggagcttgt tccgttgcgc tgatcccgac
      121 tggtatacca gtcctgggca gcatagtctg aagagctgta ggagaagaag atcgagaggc
      181 ccctcttgac cgaactgccc gtcccccaat aatagctcgt cccgtatccc gacacacccg
      241 aggcaactgt ggctgtctcg ccataagcct tgaccaccgc atttcccaag gccgtcaaca
      301 cattgttggc ggctgtcttg agtgctgtcg aacaggtgga gctggctgca atcttcgagc
      361 acagggaccc aagatcgaag tacgggtact cctggcggga atacgtgtca ctgttatcgt
      421 aatacttgac tgccgaattg cgtgttgatt cgatggaact ctttttgttc tcggtgtaca
      481 tcgcgacagc catgctgtcg acagcggtct tgagtgcgga gatctgcgag agatcgatcg
      541 cggccagagt ctccccatcg ccgacgggat tcgcctggat ctggaccttg taggcatcca
      601 cgaccaaggt cgcgaaattc accggagtga tggaagccga gccgagattc ccgaaaatgt
      661 attcgtaatc ccagccatcc ccctgttcgg tctgcatcga cccgaccata tagctggcaa
      721 gattcctgaa ctcataggcc gtctcaactg tgctcatcag acaggcatcg aatccgagga
      781 tgccgagttt ggaagaggat gtaaaccgct gagcaagagc ttgctgaagc tcgtcgagat
      841 acaggcaatc atcaccactg gcatcatccc agcaaacggc cttggccgga cgctcggccg
      901 gggtccagtc ccttgcccca ccaccatggt tccataacac aaccatgtag ttgttggccg
      961 ggaagtaggt ctttccataa ttcacaaacc aggagagcgt ggcaggatca cccatgttct
     1021 tttcaccaca gttcgcgata tgccccaaac ctgcaccgtt atcgtcaagc cgctcggaat
     1081 tgaaataggt catcgaggat gaatccggtc gcacacgata gagacgtgta tcagaccagt
     1141 cgctccctgt tgactccgtt tttgccgaag tatagccatc cgccgttgat gaaccggttg
     1201 ccctgtcaag gaggacgatc acattgatgt tgtcggagat ggagacaccg gcattggcca
     1261 ggcccttttc catctcgttc atgtcttcaa gcccggccgc ttctagattg ttgtccgcat
     1321 caagatagac catcaccgtc cagttcttga ccgtcgcact ctgtgaagag gacgaagaag
     1381 aggaggatga agaagaaacg ctggatgatg atgaactcgt tccaccagtc gtgtactcaa
     1441 gtgcgtcgat cttccatccg gccgctgcaa cgctggaatc ggaggtgaag cggatcttga
     1501 cggtatcccc ggcaacctcg atggatgtaa aagctccctt gttgcctgtg tacgtggcca
     1561 agaccgtccc gagtccgttc atgatgtaga ctttgtcgta attgttctcg gtgttgaacg
     1621 ctgagaagtg cacccgaacc ttcgatgcac cagcctgtgt cagcgtcgag gtgacatcct
     1681 gactggcagg atagttggat ggatagttcg gagatgtcag gcttttcgcg acactgaccc
     1741 agcccgacgg agcagaactg ctcgaagaaa cactggacga aaccgagctg ctcgatgaag
     1801 aagaggaaga cgaagatgaa gagacactcg acgaagaact tgtcccaccg tcatacgaag
     1861 tccaggagat atcgtccaca gccacctgtc cggccgatgt cgaagactgg atcaactgga
     1921 tggtcacact cccgctccgg ttgaccgtat aagggccagc tgtcagcaca gttgtcccgg
     1981 aggtgaccaa tgtggcaatg gtcgttccat tgaccttgac aaggagactg gtcgctgtgc
     2041 tgtaggcttt cttgtacttg aaagacaagg ttccgacgcc gttattgact gtcacactca
     2101 aagccgaggt cggggactgt cccttgccga ggacagctgt cttgcccgtc agagtcactc
     2161 cgccccaagc cgtctttgta taggcccacg accagccacc gacaccggca aagctgccgg
     2221 taacataact cgtccctgtt gccgtgcaag aatcgaatgt ttcggtgtaa ctcgtaacag
     2281 ttcttgcctt gacggcatca tcctgcgacg tccgatcgtc gactccagcg acttccgggg
     2341 caatgctgca cgctgcaagt gcagttgctg tgatggcagc gcaagcaata cggctccata
     2401 ctctcataga gaactccatt ctgtcatatc aagtttgaac agcttggagc acattctctc
     2461 acacccccga tttccgtcaa ataaattccg gtttttttct gtccagcgac cagatgccac
     2521 ccaaagcgtc tttttatact atttttaagg ggagccgtcc aaaaagcggc aaccatttct
     2581 ccggggaccc acaatggaat tcgaagccgt catcggactt gaagtacacg cacagctcaa
     2641 tacccagacc aagatctttt gttcctgcgc caaccgcttt ggagccgagc caaacaccca
     2701 gacctgcccc gtctgccagg ggcatccggg tgttctcccc gtcctcaacg gcgaagccct
     2761 gaccaaggct atcaaggccg gtctggccct tgatggatcc attgcccact actcgcgctt
     2821 tgaccgcaag aattactttt acccagacct tcccaaggcc taccagattt cacagtatga
     2881 gtttcccata gttgttggcg gtggcgtgac cgtggttcgc gaagacggga gcgaacacac
     2941 cttccccctg acccggattc atatggaaga agacgctggc aaactcgtcc atcccggaga
     3001 tgacgcagcc cattccctcg tggacctcaa tcgggccgga acgcccctga tcgaaatcgt
     3061 cagcgagccc gaactgcaca cccctgaaga tgccgccatg tatctcaccg agctccgggc
     3121 catcctgcgt tacatcgatg tctccgattg caacatggag gaaggctccc tgcgctgcga
     3181 cgccaatgtc tccatccggc ccaaaggaac cacaacactc ggcaccaagg tggaaatcaa
     3241 aaacctgaac tccatcaagg gagttgccaa ggccattgaa tacgaaatcc gccgccagac
     3301 cgaagcgctt ctttccggaa cgccgatcat ccaggaaaca cggctctttg acgctgacag
     3361 ggagatcacc ctctcgatgc gcagcaagga agaggcccac gactatcgct acttcccgga
     3421 cccggatctg gttcctgtcc tcgtcccgga agaacggatc cggacactga aagacacact
     3481 ccccgaactg cctcgcgccc gaaaaacccg gtttatcacc gagtattgcc tgaccccgaa
     3541 ggatgtcggt acactctgtg cgacccgcga actggccgat tggtttgaaa cggcggctcg
     3601 ggcgggaaag aatcccaaaa aaattgccaa ctggatccag gccgaggtgc tccgcatcct
     3661 gaacaattcc ggccagggca tcaccgcgtt ccccgtctcg cccgaaaaca ccgcagccct
     3721 gatggatctt gtcgaagatg gtgtcatcag cgggaaaatc gccaaagatg tctttgaaaa
     3781 gatgatcagc accggaaaat ccgccgagag catcatcgaa gcggatggcc tgcgtcaggt
     3841 ctccgataca ggtgccattg aacaggtgat cgacgaggtc ctggcggcaa acccggccca
     3901 ggtcgaacag ttcaagagcg gaaaaaccgc tgtcaaggga ttccttgtcg gccagatcat
     3961 gaaaaaaagc ggcgggaagg tcaatccagc catggtcaac cagattctcg agcaaaaact
     4021 ccagtcctga ccgtacccga aaaagggctg cccaataagg ttgacacacg caatccgccg
     4081 acacgcccgg gcctccgacg tgtcggcgga tcaaacgctt tcgcagagtg ccgaaataca
     4141 gttcaggata tcatggtttc tgcgaatgtc ccccggaatc gcatcacaac atcatgaaac
     4201 gcgtataagg ccgcgcgttc aggcttgcga agcgtcggcg aaacctgttt ttcataagat
     4261 accagaacac ttcctttgtc acacagaccc cgaagcccct caacgcaaca aatcctgtcg
     4321 atcccgtgac agatcttcgg gtgccttatg ccaccccggc actctgcctg agccgggcgt
     4381 ttgtggcggc cttctcgtat ccgaccacca ccggtttccc tccggccttt tccggcgctc
     4441 ccgctgtcaa gccgggcaca aatcccatcc ccaggagcgt ccggatctcg tccctctcga
     4501 tcagggacat ctggcggctc cgggcaaaag cttttgcgtc atcggaaaaa
//
LOCUS       DAOSHE010000132         4541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17108_length_4541_cov_4.296478, whole genome shotgun sequence.
ACCESSION   DAOSHE010000132 DAOSHE010000000
VERSION     DAOSHE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17108_length_4541_cov_4.296478"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLD82_04890"
     CDS             <1..925
                     /locus_tag="PLD82_04890"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOG64755.1"
                     /translation="AFRPPAVSLHTRATLKVQDGCDNHCTYCIVPAVRGSARSRPAGD
                     CLTAARELIALGYHEIVITGVNLQQYTRDGLDLPWLVRELLAIPGEVRWRLSSIEPDD
                     TTPAIVGLFDNPRLCPHLHLCLQSGSDRTLKRMGRRYTAGMFLDIVRELRRIDPLFNV
                     TGDVIVGYPGETENDFDETMATLEEARLTHIHTFQFSPREGTPAAVMDDRVPKPEMQR
                     RSEAVRNLTARTRTSYLETLLGQRETILVEKVDTGERTIRGYGEHYIPITMTFPPQVA
                     LPEANTFVPCLVSGTAPGKLQSLAGSLPGEK"
     gene            986..2359
                     /locus_tag="PLD82_04895"
     CDS             986..2359
                     /locus_tag="PLD82_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64756.1"
                     /translation="MTASGLHPMPTAVYHDLMEERMTQQSRRNTRLAIEILVLALGSC
                     CIWITSWALGFFSWLSSATRAAGIFEQVAFLLAICLVAILIFSVRRYRDLRLEMTRRR
                     EAEALMQDRDLLFRRYFELGPIGKGISAPDKTWVEINDHLCAMFGYSRDELMQKTWAD
                     LTWPDDLAKDVAHFESMMQGQSEGYIMDKRFLRSDGSVLYANMAVSCIRSPEGEVNHI
                     IATFLDVSERKRAESQVRALSQALLQSQERERQRISRDLHDNVAQDLSSLKMSLETLL
                     ADSPEATAGGQRLPGIIRVLQTTINNVRDISYNLRPPGLEQLGLIRTMAQFCDDFTRL
                     HRIDVDFDSAGLDFALLDPETEINLYRILQEALGNVRRHARATKVIVRLVASWPNIIL
                     RIQDNGCGFNAGDVLGGGGESERHMGLRNMRERVTLLGGKCTLESLPGEGTRILIEIP
                     QKEPDHG"
     gene            2352..3026
                     /locus_tag="PLD82_04900"
     CDS             2352..3026
                     /locus_tag="PLD82_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG64757.1"
                     /translation="MAEPVRVLIIDDHPLYREGLKTLLGRSERFGICAEAGTASEGLE
                     AALACKPQIILTDLSLPDRGGLDLVEELIQRVPDAKILVVSMHSKIEYIAGAFQRGAL
                     GYLVKDKAAENLVTGIEAILAGTRFLEAPHSMEEVETVISGTHAGVNRQVAYSLLSPR
                     EQEVLRLLAEGLSNKEVADRLFISTKTAEHHRANIMAKLDVHNMVELVRYAARLGLVD
                     MQSWRD"
     gene            complement(3415..4404)
                     /locus_tag="PLD82_04905"
     CDS             complement(3415..4404)
                     /locus_tag="PLD82_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG64758.1"
                     /translation="MQIFRRIKRIFDSRPVACHLYVTEQCNLHCGYCTEFDNTIPHPD
                     LATVKRWVDKLADLGCMRIGIQGGEPLKHPDIVEIVRYIKSKKMGCSMSSNAFLLTPE
                     LVAGLEDAGLDSIHVSIDSMDPTESTKKCIRLVEPKLMMLRASKIPLHLTSVLFKESL
                     PQLPEVFAWAREKGLSIKAHLIHAGTAGEYTVEQGDRKALGDFIDWQIREKKNGRNIR
                     TMFNVLRYQKNLTEKNNYRWKCLAGYKFLFVSARGEFWLCSMQRRPGTDILALTKKDL
                     KANNCKKPCQKGCGVYCVVSESLVNNKPLSFGLRELGSILGGLFGRPFRRKSA"
BASE COUNT          907 a         1479 c         1323 g          832 t
ORIGIN      
        1 cgccttccgg ccaccagcgg tctccctgca tacccgggcc accctcaagg tccaggacgg
       61 ctgcgacaac cactgtacct actgcatcgt gccggctgtc cgcggttccg cccgaagccg
      121 tccggccgga gactgcctga ctgctgcccg ggaactgatc gcgctcgggt atcacgaaat
      181 cgtgatcacg ggtgtcaatc tccaacagta cactcgggac gggctggatc tgccctggct
      241 ggtccgggag ctccttgcca ttccaggcga ggtccgctgg agactttctt caatcgaacc
      301 cgatgacacg accccggcca ttgtcgggct cttcgacaac ccccggctct gcccccatct
      361 ccatctctgc ctccagagcg gctctgaccg gaccctgaag cggatgggcc ggcggtacac
      421 ggccggaatg ttcctcgata ttgtccgtga gctgcgccgg atcgatcccc tcttcaatgt
      481 cacgggcgat gtgatcgtcg gctatcccgg ggagacggaa aacgatttcg atgaaactat
      541 ggccactctg gaagaggccc ggctgaccca tatccatacc ttccagttct ccccccgcga
      601 agggactccg gcggccgtca tggacgatcg tgtccccaag cctgaaatgc agcgcaggag
      661 cgaggcagtc cgcaacctga cagcacgcac acggacaagc tatctcgaaa cactcctcgg
      721 gcagcgggaa accatcctgg tcgaaaaggt cgacaccggg gaaaggacaa tccgtgggta
      781 tggagaacat tatattccga tcactatgac gtttcccccg caggtcgcac tccctgaagc
      841 caataccttc gttccctgcc tggtcagcgg aaccgcaccc ggcaaactcc agtcactggc
      901 gggcagcctc cccggagaaa aataggggga atcccccatc ctcttccccg tcgaatacct
      961 gtattatcac agaattcgga gaaacatgac tgcgtcaggc ttgcacccca tgccgactgc
     1021 ggtctatcat gatctgatgg aagaacggat gacacagcaa tcccgcagga acacaaggct
     1081 cgccatcgag atcctggttc tcgccctggg atcgtgttgc atctggatca cctcctgggc
     1141 cctcggtttc ttctcctggc tctcgagtgc cacacgggcc gccggaatct tcgagcaggt
     1201 cgccttcctc ctcgcgatct gtctggtcgc catcctgatc ttctccgtcc gccgctatcg
     1261 cgacctcaga ctggagatga cacgccgcag ggaagccgaa gcattgatgc aggatcggga
     1321 cctgctcttc cgccgctatt tcgaactcgg accgatcggg aaaggaatct ccgcaccgga
     1381 caagacctgg gtcgagatca atgaccatct ctgtgcgatg ttcggctact cgcgggatga
     1441 actgatgcaa aagacctggg cagacctgac ctggccggat gatctcgcca aggatgtcgc
     1501 ccacttcgaa agcatgatgc aggggcagag tgagggctac atcatggaca aacgcttcct
     1561 gcgcagtgat ggctcggtcc tctacgcgaa catggctgtc tcctgcatcc gcagtcccga
     1621 aggcgaggtc aaccatatca tcgcaacctt tctggatgtc agcgaacgaa aacgggccga
     1681 gtcccaggtc cgggcgctct cgcaggccct cctccagtcc caagagaggg agcgacagcg
     1741 gatctcgcgg gatctccatg acaatgttgc ccaagatctc tcctccctca agatgtccct
     1801 cgagaccctg ctggccgact cgccggaagc caccgcagga ggacaacggc ttcccggaat
     1861 catccgcgtc ctgcagacaa cgatcaacaa tgtccgggat atctcctaca atctccgccc
     1921 gcccgggctg gaacagctcg gcctgatccg gaccatggcc cagttctgcg acgacttcac
     1981 gcggctgcac cgcatcgacg tcgatttcga ttcggccgga ctcgacttcg cgctgctcga
     2041 tccggaaacc gagatcaacc tctaccgcat cctgcaggaa gccctcggca acgtccggcg
     2101 gcacgcccgg gcgacgaagg tcatcgtccg gctcgtcgcc tcgtggccga acatcatcct
     2161 gcgcatccag gacaacggct gcggcttcaa tgccggcgac gtgctgggcg ggggcggcga
     2221 aagcgaacgg cacatgggcc tgcgcaacat gcgcgaacgc gtgaccctgc tcggcggaaa
     2281 atgcaccctg gaatccctgc ccggagaggg cacccgcatc ctgattgaga taccgcagaa
     2341 ggagcccgac catggctgaa ccggtccgcg tgctgatcat cgacgaccat ccgctctacc
     2401 gcgaaggact gaagacgctg ctcggccgca gcgagcgctt cgggatttgc gccgaagccg
     2461 gcacggccag cgaagggctc gaggccgccc tggcctgcaa gccgcagatc atcctgaccg
     2521 atctctcgct gcccgaccgg ggcggcctcg acctggtcga ggagctgatc cagcgcgtgc
     2581 ccgacgcgaa aatcctcgtc gtcagcatgc actccaagat cgaatacatc gccggcgcct
     2641 tccagcgcgg cgccctcggc tacctcgtca aggacaaggc cgcggagaat ctcgtgaccg
     2701 gcatcgaggc gatcctcgcc ggaacgcggt tcctcgaggc cccgcactcg atggaagagg
     2761 tggaaacggt gatcagcggc acccatgccg gcgtcaaccg ccaggtcgcc tacagcctgc
     2821 tctcgccgcg cgaacaggag gtgctccggc ttctcgccga ggggctctcc aacaaggagg
     2881 tcgccgaccg cctgttcatc agcacgaaga ccgccgaaca ccaccgggcg aacatcatgg
     2941 ccaagctcga tgtccacaac atggtcgaac tggtccgcta cgcggcccgt ctcggcctcg
     3001 tcgacatgca aagctggcgc gactgaatgg ttgttcctgt aaggtgtgtt cctcctgctc
     3061 gtcggaacgg ttcaatctgt cggaacggtt caatctgaac cgcgccgcga ggcgcagacc
     3121 acttccacca cccgggaaat ggttgttcct gcaaggtgtg ttcctcctgc tcgtcggaac
     3181 ggttcaatcc gtcggaacgg ttcaatctaa cccgcgccgc gaagcgcagg acattttagc
     3241 cgtaaaagca acaaccgcca gcccggtcag gtttgaaaaa acccgacggc agggcgcaaa
     3301 ccgccacgtc gtcgggtaaa tggtctttgc cggagaacgg ttgttccccc agccgccgga
     3361 acgatccgaa cggaagcgcg tcgcgtggcg cgaaccggac cgtccctcgc cgggtcaggc
     3421 gctcttgcgg cggaagggcc ggccgaacag gccgcccagg atgctgccca gttcgcgcag
     3481 gccgaaggaa agcggcttgt tgttgaccag ggattcggag acgacgcaat agacgccgca
     3541 gcctttctgg cagggctttt tgcagttgtt ggccttgaga tccttcttcg tcagcgcgag
     3601 aatgtcggtc cccggccggc gctgcatgct gcagagccag aactcgccgc gggccgaaac
     3661 aaagaggaac ttgtacccgg ccaggcattt ccatcggtag ttgttcttct ccgtcaggtt
     3721 cttctggtag cgcaggacat tgaacatcgt acggatgttg cggccgttct tcttctcgcg
     3781 gatctgccag tcgatgaaat cgccgagggc cttccggtca ccctgttcga ccgtgtattc
     3841 gccggcggtg ccggcgtgga tcaggtgggc cttgatcgag agccccttct cccgggccca
     3901 ggcgaacacc tcgggcagct ggggcagtga ttccttgaac aggacgctgg tcaggtgcag
     3961 cggaatcttc gaggcccgca gcatcatgag cttcggttcg accagccgga tacacttctt
     4021 cgtcgactcg gtcggatcca tgctgtcgat cgagacgtgg atcgaatcga gcccggcatc
     4081 ctcgagcccg gcgaccagtt ccggcgtcag caggaaggcg ttcgaggaca tgctgcaacc
     4141 catcttcttc gacttgatgt accggacgat ctcgacgatg tccgggtgct tgagcggctc
     4201 gccgccctgg atgccgatcc gcatgcagcc caggtcggcc agcttgtcga cccagcgctt
     4261 gaccgtggcc aggtccggat gcggaatcgt attgtcgaat tcggtgcaat agccgcagtg
     4321 aagattgcac tgttcggtga cataaagatg gcaggcaacg ggacggctgt cgaaaatccg
     4381 tttgatgcgg cggaatatct gcacgggtga acctctcacg atgggctttt cttttccaaa
     4441 aataacgccg gtccgccggt ccgtatcaaa caaaatgccc gggacagacc ggaaaaaact
     4501 gaccggcccg cgaccggaag cgataccttt acggaacaaa g
//
LOCUS       DAOSHE010000133         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17308_length_4510_cov_3.684400, whole genome shotgun sequence.
ACCESSION   DAOSHE010000133 DAOSHE010000000
VERSION     DAOSHE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17308_length_4510_cov_3.684400"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLD82_04910"
     CDS             complement(<1..245)
                     /locus_tag="PLD82_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64759.1"
                     /translation="MNWKKSLRVMFSIWTLGTFLVICLPAIFWISSEMLDLRRSFADS
                     LSGQKASDIKNRTIQRMKSDLSSCFQLAESPAVRSWL"
     gene            complement(268..2199)
                     /locus_tag="PLD82_04915"
     CDS             complement(268..2199)
                     /locus_tag="PLD82_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG64760.1"
                     /translation="MEWKPGLRVSFSLWTLAFFLVFFLPAIFWTGNELLDLRQHFAER
                     YSRQRAIDIRERTEQYMKGNISSILQLADSPLVSQWMANEQDEQARSMAFASFDLFSN
                     TMENNPGVSAAVKKSLRYYFRNRFQTILSPDNPVDDWFFKSLDRQERFALDIDFDPTL
                     KLTRFWINATVRDQSGNKTLGIISAGIDITVFLKTMLTTGIDGAFIAFFDAQGIITGY
                     RDIRFITRSSINTILDVPEERETLAQAMETLRKRGDTFKELSLDHRGTRYHASLVYIP
                     VIKWYALVFIDTTRVIRLERFIPILATVLVSIFLLFGSMLLYTTLRLIRPIRRVTDVM
                     KRIENGEEDARVPVTHDDETGDLERACNAMANEISAYTSRLEEMVRHKTDELVRKNHE
                     LVDSLTYARLIQSGILPKRTHIAKRIPQHCLIWRPQNIVSGDFYWYKEIGESLLIAVV
                     DCAGHGIPGAFMSMTANSILDHIVWYIHDNPAAIILELDRILASSLDNDSGEPFPEEH
                     KGIEIALVMLTPSEQTVTVACTGIPVYLCDSVSVEIVSPSGGAVGLVSRNRSATPVTH
                     TRKMVSGERIFISTDGLFSQTGVDGSLFGRPRFQEILTTSLSMTLEEQRNHILLSFDR
                     FRQSLPQDDDITVFGLEIP"
     gene            complement(2372..3748)
                     /locus_tag="PLD82_04920"
     CDS             complement(2372..3748)
                     /locus_tag="PLD82_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64761.1"
                     /translation="MKTAIRTVQIFALVGVLFLGNLSAAAQEAREPSWVCLTLPNGIV
                     AWHRQLPAARTISIRVCVPSASGLNPAAPANDRLLFSLMETAGEEYPRARLDRIRDEG
                     ALDISGTSGLIFSSLSVSAIADRGEEAAMILSSLLYKPARDRQIFSELRTRAVNELHW
                     RDGNAWSALDPHLQLAVHGKTVDGAFYGLSDSALATTQLEDVLDHHRKILAFEGTFLV
                     SCGPQSAAGMAAVLARSFTRPTLRPSPRPLARAPFPAATNAVHVFPHEAASSAWAKGI
                     FAAPRPGSEGYAAFVLTSRIWSEMLMDSVRSRNGLVYSVWASSPSREQHASAEFVLYR
                     CQDIQRALDLAAESLRKLREGIAVLPGPESPARSIADVLPATRNQILASIALQNITPA
                     ERTSSFASWLLASGDPKAFDRLPDAIRAVSPEDIRKTAQRFYDRMHWGVTLSPKAVTN
                     FCGLRWNP"
     gene            complement(3745..>4510)
                     /locus_tag="PLD82_04925"
     CDS             complement(3745..>4510)
                     /locus_tag="PLD82_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64762.1"
                     /translation="CMLSLSGAIDEREALQLVSKHFSRWKRGTPRPVTRPLQFKPRTD
                     RSRNLVLGGFTNPGMLRVLLLWPGPSVLSNRNETLTADVLSYYLSEKSGGFLASLTTN
                     TALFSTDTAGFTFSTGRYTSEFMFQGEIPATNIPGLLARASLLQNTVRQALARIAEGK
                     ARISPESIRRIASRIINDRIFRHEKPPALLQDVSWWWCTADLAYWQHYDRGIATVTPR
                     ALQRLARDWLAKPPLPVLWAHDSLRAALEQQGGPNP"
BASE COUNT          899 a         1213 c         1478 g          920 t
ORIGIN      
        1 ttcagccagg aacggacagc cggggattcg gcaagctgga agcaggatga gaggtcgctc
       61 ttcatgcgtt ggatcgtgcg gttcttgatg tcggaggctt tctgtccgct caggctgtcg
      121 gcgaaggaac gccgcagatc gagcatctcg ctgctgatcc agaagatggc cgggagacag
      181 atgaccaaaa acgttcccag tgtccagatg ctgaacatga ccctcaggct ttttttccag
      241 ttcatcgtat ggtcctccgg ctgtcgctca tggaatctcc agtccaaaaa ccgtgatatc
      301 atcatcctgt ggaagcgact gacggaagcg gtcaaacgag agcaggatgt gattgcgttg
      361 ttcctcgagt gtcatcgaga gggatgttgt caatatctcc tggaagcgcg gcctgccgaa
      421 gagggaaccg tctaccccgg tctgggagaa gagtccgtcc gtcgagatga agatcctctc
      481 gccggagacc atcttccggg tgtgcgtgac aggggtcgcg gaacggttgc gggacacaag
      541 gccgactgcg cccccggagg gcgagacgat ctcgacgctc acgctgtcac agagatagac
      601 ggggattccg gtacaggcga cagtgaccgt ctgctcggaa ggagtgagca tgaccaaggc
      661 gatctcgatt cccttgtgtt cttcggggaa gggttccccg gaatcattgt caagggagct
      721 cgcgagtatc ctgtcaagct cgagaatgat ggcagcgggg ttgtcgtgga tgtaccagac
      781 gatatggtca aggatcgaat tggctgtcat cgacatgaag gctcccggga tcccgtgtcc
      841 ggcgcagtct acaaccgcga tcaggagact ctctccgatt tccttgtacc agtaaaaatc
      901 accgctcacg atattctgtg gccgccagat caggcaatgc tgcgggatcc gcttcgcgat
      961 gtgcgtccgt ttggggagga tcccggactg gatcaagcgg gcgtaggtca ggctgtcgac
     1021 aagctcatgg tttttccgga cgagttcgtc cgtcttgtgc cggaccatct cctcgaggcg
     1081 ggatgtgtag gcgctgatct cgttggccat cgcgttgcag gcccgttcga gatcacctgt
     1141 ttcgtcgtca tgggtcacgg gaacccgagc atcctcttcg ccgttttcaa tccgtttcat
     1201 gacgtccgtg acgcgccgga tgggccggat cagacggagc gtcgtataga ggagcatgct
     1261 gccgaagagg aggaagatcg aaaccagcac agttgccaaa atcgggatga agcgttcgag
     1321 acggatcacc ctggttgtgt caataaagac cagggcgtac cacttgatga ccgggatgta
     1381 gaccaggctg gcgtggtagc gtgtgcctct gtggtcgagg gagagttcct tgaatgtgtc
     1441 tccgcgtttc cggagggtct ccatggcctg tgcaagggtc tctcgctcct cggggacatc
     1501 cagaatcgtg ttgatgctgc tcctggtaat gaagcggatg tcccggtatc cggtgatgat
     1561 tccctgtgcg tcgaaaaaag cgatgaaggc cccgtcgatt cccgtcgtca gcattgtttt
     1621 gagaaaaacc gtgatgtcga tgcctgcact gatgatgccg agcgtcttgt tcccgctctg
     1681 gtcgcggacg gtggcgttga tccagaaccg ggtcagcttg agggtcgggt caaaatcgat
     1741 gtccagggca aagcgctcct ggcggtcgag ggacttgaag aaccagtcgt cgacgggatt
     1801 gtcaggcgag aggatggttt ggaacctgtt ccggaaataa tagcgcaacg attttttgac
     1861 ggccgcactg acaccgggat tgttttccat ggtgttgctg aagagatcga aactggcaaa
     1921 ggccatgctc cgggcctgtt catcctgttc gttggccatc cactggctga caagcgggga
     1981 gtcggccagt tggaggatgg aagagatatt ccccttcatg tactgctcgg tccgctcccg
     2041 gatgtcgatg gcccgttgtc tgctgtagcg ctcggcaaag tgctgtcgca gatcgaggag
     2101 ctcgttcccc gtccagaaga tggcgggaag aaagaacacg agaaagaaag caagggtcca
     2161 gagactgaag gagactcgaa gtcccggttt ccattccata catcccgcct tttcgacagt
     2221 atagcagaaa acccggccgg tgcaagaagg agtttttgga aacggaaaaa gccggatgtt
     2281 cccgggatct tcggtatgca tccggcgttg ttttttggtg aacaacctcg ggccggtttg
     2341 gcctgagact gtccacattg agaagagccg gtcagggatt ccagcgcagc ccgcaaaaat
     2401 tcgtgacggc cttgggcgag agcgttactc cccagtgcat cctgtcataa aacctctgtg
     2461 ccgttttccg gatgtcttcg ggggagacgg cccggatcgc gtcaggaagc cggtcaaagg
     2521 ctttgggatc gccactggcc aggagccaac tggcaaaact ggatgtccgt tcggccgggg
     2581 tgatgttttg cagggcaatg ctggcgagaa tctggtttct ggtcgccggc aggacatcgg
     2641 cgatcgagcg ggccgggctc tccgggccgg gaaggacggc gatcccctca cgcagtttgc
     2701 gcagactctc cgcggcgaga tccagggccc gctggatgtc ctgacagcgg tagaggacga
     2761 actcggcact ggcatgctgc tcgcgactgg gtgacgaggc ccagacactg tagaccaggc
     2821 cattccgcga gcggacgctg tccatcagca tctcagacca gatccggctg gtcaggacaa
     2881 aggcggcata cccttctgag cccggtctgg gagcggcaaa gatccccttt gcccaggccg
     2941 agctggccgc ctcgtgcggg aagacatgga ccgcattggt cgccgccgga aagggggctc
     3001 gcgcgagggg gcggggtgag ggtcgcaggg ttggacgggt aaagctgcgc gccaggacgg
     3061 ccgccatccc ggcggcggac tggggtccgc aactgaccag aaatgtcccc tcaaacgcca
     3121 ggattttccg gtggtggtcg aggacgtctt cgagttgtgt cgtagcgagt gccgagtcac
     3181 tcaggccgta aaacgcgcca tccacggtct tgccatggac agccagttga aggtgcggat
     3241 cgagggcgga ccaggcattc ccgtcccgcc agtgaagttc attgacagcc cgggtccgga
     3301 gttcggaaaa aatctgcctg tccctggccg gtttgtagag gagggaagac agaatcatgg
     3361 cggcctcttc tcccctgtcg gcgatggcac tgaccgagag gctcgaaaag atcagcccac
     3421 tcgtgcctga gatgtccaga gccccctcgt cccggatccg gtccaatctc gcgcgcggat
     3481 actcctcgcc cgcggtctcc atcagggaga agagcaggcg gtcgttggcg ggtgcggctg
     3541 gattgagtcc cgatgcggag gggacacaga cccggatgga gatggtccgg gccgccggaa
     3601 gctggcggtg ccaggccacg atcccgttgg gaagggtcag acagacccat gagggctctc
     3661 ttgcctcttg ggcggccgca ctcaggttcc ccaaaaaaag gaccccgacc agtgcgaaaa
     3721 tttgaacggt tcgaattgcg gttttcacgg gtttggtcct ccctgttgtt ccagcgcagc
     3781 ccgcaaggaa tcatgggccc agaggacggg cagtggcggt ttcgcgagcc agtcccgggc
     3841 caggcgttgc aatgcccggg gcgtgacggt tgcgatcccc cggtcgtagt gttgccagta
     3901 ggccaggtcg gccgtacacc accaccagct cacatcctgg aggagggccg gtggtttttc
     3961 gtgcctgaag atgcggtcgt tgatgatccg cgaggcgatc cggcggatac tctccgggga
     4021 aatcctcgcc ttcccctccg cgatccgggc cagagcctgg cggacggtat tctggaggag
     4081 ggaggcccgt gccaggagtc ctggtatatt ggttgccggg atttccccct ggaacatgaa
     4141 ttccgaggtg tatcgtccgg tcgagaaggt aaacccggca gtatcggtcg aaaagagggc
     4201 ggtatttgtt gtaagcgagg ccaaaaatcc cccggatttt tcagagagat aataggacag
     4261 tacatcggcc gtcagggttt cgttcctgtt cgagagaacc gaaggacctg gccagaggag
     4321 gaggacgcgg agcatgccgg gattggtgaa tccgcccaga accaggtttc tgctccggtc
     4381 cgtgcgcggt ttgaactgca gtggtcgggt gaccggtcgg ggcgtcccgc gcttccagcg
     4441 ggaaaaatgt tttgacacca gttgcagggc ttcccgttca tcgatggcac ccgagaggga
     4501 gagcatgcag
//
LOCUS       DAOSHE010000134         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17474_length_4483_cov_4.814589, whole genome shotgun sequence.
ACCESSION   DAOSHE010000134 DAOSHE010000000
VERSION     DAOSHE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17474_length_4483_cov_4.814589"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1353
                     /locus_tag="PLD82_04930"
                     /pseudo
     CDS             133..1353
                     /locus_tag="PLD82_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
     gene            1438..3012
                     /gene="cimA"
                     /locus_tag="PLD82_04935"
     CDS             1438..3012
                     /gene="cimA"
                     /locus_tag="PLD82_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOG64763.1"
                     /translation="MARIAVYDTTLRDGRQGAGISFSLVDLVAVAHRLDELGVDYIEA
                     GWPGSNPKDIEFFEALKHEHFRHARIVAFGSTRHARSRVAEDQVLRCLVDSGAPVLTV
                     FGKSWDLHVREVFNITEAQNIDMIRESVAWLKEQGRTVIYDAEHFFDGFRDNPDFALA
                     TVRAAAEAGADNISLCETNGGMLPFDIDEITRIVAAEVGTPLGIHCHNDSGCGVANTL
                     AAVHAGAVLVQGTINGLGERCGNANLCTIIPDLAVKMQHDVLAGPACLERLTDTSRII
                     AELANLPHDPSFPWVGANAFAHKAGVHVNAVQKNPRTYEHVEPECVGNRRHILISDYS
                     GKSSVVAKAREFNLDLEDKDLPGQVIDLVKRLENEGFQFEGADASFELLLRRAMGLYK
                     PGFELKGFRIINHKVENKASVNEATIKVVVDGVEEMAVAEGDGPVNALDCALRKVLEK
                     FYPILKNMSLIDYKVRILNGGEATAARTRVLIESRYGDEVWGTVGVSENIIEASWQAL
                     VDSIEYLLMKHFDEEG"
     gene            3027..3857
                     /gene="murI"
                     /locus_tag="PLD82_04940"
     CDS             3027..3857
                     /gene="murI"
                     /locus_tag="PLD82_04940"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG64764.1"
                     /translation="MDQRAIGVFDSGVGGLTVAQSIIELLPDERIVYFGDTARVPYGS
                     KSKNTIIRFAEENTHFLLSKDVKCIVVACNTATAIALPHLQEMFRIPIIGVVTPGAAR
                     AVGTTRNHRIGVIGTMRTVASGAYSQAITGMDPDAVVFARACPLFVPLIEEDWIGHAA
                     TRLVVREYLDELVSREIDTLVLGCTHYPLLADVIGACYPDLALVDSAVSTAETVRTIL
                     TESGMLAEKAEGERIHIWLSDYSESFHELAGRILHRDIDNVLFTVRLEELVQGMAGKL
                     "
     gene            3869..>4483
                     /locus_tag="PLD82_04945"
     CDS             3869..>4483
                     /locus_tag="PLD82_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG64765.1"
                     /translation="MRHVRYTLLAVMAGILVAVHLQAVIVEKIAIKVNDEIITQAEII
                     VREQEILAEFARAQKQPPPNLRQFVTDQLVNDRLITQLAKKEGVIVGHAEVEERIKRI
                     LKGQSTDLETFKANLVREGISYDAFYEQTRKKIVMQKLFFKSPGASAITPDDREIEDF
                     YKKNAPQEMHLMHCYVSVPVGASFAVRNAKERQIKAIEAALAANP"
BASE COUNT          991 a         1222 c         1339 g          931 t
ORIGIN      
        1 gtggcggaga gggtggggat accggacgcc atcacccaaa aaaaacagat cgccggcaga
       61 tgtaaattct tgcatgccat tgattgtcag gatatatttt cccaaatcgg caggaaggtc
      121 tgcaagcagg ggatttgtgt ggacatactc gtacagaaat atggcgggac ctcggtgggc
      181 tcggtggagc gcatcaaaaa tgtggcccgg cgcatcatcc gcagcaagga aaaaggctat
      241 tgtgttgtcg tcgtcgtttc ggcgatgagt ggtgagacca acagactgat cgatctggcc
      301 cagcaggtga cagccgaacc ggccaaacgc gagatggaca tgctcgtctc gaccggtgag
      361 caggtctcga ttgccctcct ggccatcgcg ttgcaggata tgggacatcc cgccatctcg
      421 atgacggcgg cccaggtcgg gatccggacc gatgccaatt tcatgaatgc acggatccag
      481 catatcgacg acaagccgat gaaggccgag ctcaaaaaag gccggatcgt gattgtcgcc
      541 ggtttccagg ggatcgatgt cggaaacaat attacaaccc tgggacgcgg tggttcggat
      601 acctcggcgg tggcccttgc ggcggcgctc aaggcaaagt attgcgagat ttacacggac
      661 gtggatggtg tctatacagc tgaccccaac aaggtgcccg atgccaggat gctgcgcgag
      721 atttcctacg aggaaatgct cgagctggcc agtcttggtt caaaggtgct ccattcgcgg
      781 tctgtcgaga tcgccatgaa ttacaatgtc gtactccatg tgagatcatc tcttaatgac
      841 aatgagggga ccctggtcgt gaaagaaacc aaagaaatgg aaaagatccg catcagcggg
      901 gtgacatcga agacggatga ggcacgggtc acgatctatg atctggatga caaacccggt
      961 attgctgccg ctgttttcag cgagctggct gccagcagga tcaatgtcga catgattgtc
     1021 cagtccagcg ccctcgaaaa gacgcatacc aacaggattt cctttaccgt caaaaagtcg
     1081 gacatcaagg ccgtcgaggc tgttgtcgag aagctgcgca agtccgggac ggtcaacgga
     1141 aagcatctgg ttgacacgaa aatcgggatc gtctcgattg tcggtgtcgg aatgaagagc
     1201 cataccggtg tcgcctcgca gctcttcgcg atcctggcaa aaaacaaaat caatatcgaa
     1261 atgatttcta cttccgagat caagatctcc tgtgtgatcg ccgaggatcg ggttcttgac
     1321 gccgtccggg cgatccacaa ggattttggc ctgggtgagg gatccgggcg gtcggctgcc
     1381 aagcccgcag ccaaaaagac ggcggcacaa cctgccgcaa agaaaaaggg gtaagccatg
     1441 gcgcgcattg ccgtctacga cacaacgctt cgcgacgggc ggcagggcgc gggcatctct
     1501 ttcagccttg ttgacttggt cgccgttgcc catcgcctgg atgaactcgg ggtcgattac
     1561 atcgaggcgg gctggcctgg ttccaacccc aaggatatcg agttttttga ggcgctcaag
     1621 catgagcatt tccgccatgc ccggatcgtg gctttcggga gtacgcgcca tgcccggagc
     1681 agggtggccg aggatcaggt cttgcgctgc ctggtggaca gcggcgctcc cgttctcacc
     1741 gtgttcggca aatcctggga cctccatgtc cgtgaggtct tcaatatcac tgaagcacag
     1801 aatatcgaca tgatccggga gtcggtggcc tggctcaaag agcagggccg gaccgtcatc
     1861 tacgatgccg agcatttttt cgacggattt cgtgacaatc ccgactttgc cctggccact
     1921 gtccgcgcgg ccgccgaagc cggtgccgac aacatctccc tctgtgagac caacgggggg
     1981 atgcttccct tcgatatcga cgagataacc cggattgtcg ctgccgaggt gggcacgcct
     2041 ctcgggattc attgccacaa tgactccggg tgcggggtcg ccaataccct ggcggccgtc
     2101 catgccgggg ccgtgctcgt ccaggggacg atcaatgggc tcggagagcg gtgcggaaat
     2161 gccaatctct gcacgatcat tcccgacctg gctgtcaaga tgcagcatga tgttctcgcc
     2221 ggtccggcct gtctcgagcg tctgaccgat acctcccgca tcatcgccga actggccaat
     2281 cttccccacg atccgagctt tccctgggtg ggcgccaacg cctttgccca caaggccggg
     2341 gtccatgtca atgccgtcca gaaaaatccc cggacctacg agcacgtcga accggaatgt
     2401 gtcggaaacc gccgccacat cctgatctcg gattattccg ggaagagctc tgtcgtggcc
     2461 aaggcgcggg agttcaatct tgatctcgag gacaaggatc tccccggtca ggtcatcgat
     2521 ctcgtcaagc gtctcgagaa tgagggcttc cagtttgaag gggctgatgc cagttttgag
     2581 ctcctcctgc gacgggccat gggactctac aagccggggt ttgaactcaa gggcttcagg
     2641 atcatcaatc acaaggtcga aaacaaggcc tcggtcaacg aggcaaccat caaggtcgtt
     2701 gtggatggcg ttgaagaaat ggctgtagca gagggggatg gtcctgtcaa cgccctcgac
     2761 tgtgccctgc gcaaggtcct cgaaaaattc tatcccattc tcaagaatat gagtctgatc
     2821 gactacaagg tgcgtatttt aaatgggggc gaggctacgg ccgcccggac ccgggtcctt
     2881 atcgagagca ggtatggcga cgaggtttgg gggacggtcg gtgtctccga aaacatcatc
     2941 gaagcatcct ggcaggcact cgtcgattcg atcgagtacc tcctcatgaa acacttcgac
     3001 gaggaaggct gaaaagcggc tctggaatgg atcagcgagc gataggggtc tttgattccg
     3061 gcgtgggcgg gctgacggtg gcccagtcca tcatcgagct tttgccggac gagcggatag
     3121 tctacttcgg ggacactgcg cgggtgccct atggctcgaa gtcgaaaaac accatcatcc
     3181 gctttgccga ggagaatacg cacttcctcc tctccaagga tgtcaagtgc atcgtcgttg
     3241 cctgtaatac ggcgaccgcg atcgcgcttc cccatctcca ggagatgttc cggattccca
     3301 tcatcggtgt ggtgacaccg ggagccgccc gtgccgtcgg gacgacccgc aaccaccgga
     3361 tcggtgtgat cggtacgatg cgcacggttg catccggggc ctacagtcag gcgattaccg
     3421 ggatggatcc ggatgcggtc gtcttcgccc gggcctgccc gctcttcgtg cctcttattg
     3481 aagaggactg gatcggacat gccgcgaccc ggctggtggt gcgggaatat ctggacgagc
     3541 ttgtttcccg ggagattgat acactggttc tgggttgcac ccactacccg ctccttgcgg
     3601 atgtgatagg cgcgtgttat cctgatctgg ccctggtgga ttcggctgtt tcaacggcgg
     3661 agaccgtgcg cacaattctc accgagtcgg gcatgctggc ggagaaagcg gaaggggagc
     3721 gcatccatat ctggctctcc gactattccg agagttttca cgaacttgca gggaggatcc
     3781 tgcatcgtga catcgacaac gtcttgttta cggtccggct tgaagaactt gtccaaggga
     3841 tggcagggaa actatgaatg ggggtcgcat gcgacacgta cggtacactc tcctggctgt
     3901 gatggccgga attctggttg cggttcatct acaggccgtg atcgtcgaga agattgccat
     3961 caaggtcaat gacgagatca tcacccaggc cgagatcatt gtccgggagc aagagatcct
     4021 ggcggaattt gcccgtgcac aaaaacagcc tccgcccaat ctcaggcagt ttgtcactga
     4081 tcagctcgtc aatgaccggc tgattaccca gcttgccaaa aaagagggcg tgatcgtcgg
     4141 acatgccgag gtcgaggaac gcatcaaacg catcctcaag gggcagagta cggatctcga
     4201 gacattcaag gcgaacctgg tccgtgaggg gatcagctat gatgcgttct acgagcagac
     4261 ccgcaaaaaa attgtcatgc aaaagctttt tttcaagagt cccggtgcct cggcgatcac
     4321 cccggatgac agggagatcg aagatttcta caaaaaaaac gcccctcagg aaatgcatct
     4381 catgcattgc tatgtttccg ttccggtggg ggccagtttc gccgtacgga atgccaagga
     4441 gcggcagatc aaggccatcg aggcagccct ggctgccaat ccc
//
LOCUS       DAOSHE010000135         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17557_length_4470_cov_3.615855, whole genome shotgun sequence.
ACCESSION   DAOSHE010000135 DAOSHE010000000
VERSION     DAOSHE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17557_length_4470_cov_3.615855"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="metK"
                     /locus_tag="PLD82_04950"
     CDS             <1..898
                     /gene="metK"
                     /locus_tag="PLD82_04950"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG64766.1"
                     /translation="SCAVMSSLHGQSPDIARGVDSSKDKEQGAGDQGMMFGYACNETP
                     ELMPLTITLAHKLVQELARMRKSGEIEHIRPDSKSQVTVEYVDGKPKRIDAIVLSTQH
                     DPFTGGYSYKKLRADIEEKLIKKVVPKELIDSKTKIYVNPTGMFVVGGPHGDSGLTGR
                     KIIVDTYGGFGAHGGGAFSGKDPSKVDRSAAYMGRYIAKNIVASGLAERCLVQLAYAI
                     GVADPVSVFVDTYGTGKVSDEQLTEAVRSVFKLKPAEIIRSLDLLKPVYRQTAAYGHF
                     GRKGFSWEKTDKAAELKKCFKK"
     gene            976..2289
                     /locus_tag="PLD82_04955"
     CDS             976..2289
                     /locus_tag="PLD82_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64767.1"
                     /translation="MRGTRYLALALLVGMFFGCNEPIPDADVQAAKQAIAQAESAKAD
                     KYDPDNLSKAKDAYRDSLKRIADSDTGNAREAALDAKRIADLATDNARKKVAEDTTAS
                     AERMIQEAIVKKAEYLAADDLARARAKLEDAKKGIADGKWLEAYTDAENSLEAAGRAK
                     DNAVRKMSELAKAVADAEDAVRRADANEVVREFAKTELDAAKLSLQKTGEEKKKVDDP
                     TTIQADSADTRNRLAATAYADAMKLAHETVNKANDAVRIALEREREFYKKKAEQKMDE
                     AKKLLEDIRKLKEQGLIKQQVIPAPVVPEGETVSQTNIGMTNLTSLSNMSNLDKYNAA
                     LEALKKAEETYTNENFVTSVRNSEEAIRIAKLVKETGKTPPDELKYYTVLIPEARDCL
                     WRIAGYPFVYGDPRLWPKIWKANKHLIVDPDLIYPDQKFVIPAKK"
     gene            2745..3290
                     /locus_tag="PLD82_04960"
     CDS             2745..3290
                     /locus_tag="PLD82_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64768.1"
                     /translation="MRFLVLYTLIALALRAPAVLAAADPLSPQVSSSIDRDRPALGET
                     FSASIRVILPGSETNACVIFPSNVESRGILSLQREAQTTRFLDGSNGQRRIESMVYVF
                     KTEHAGSLSLEWPDVVVFDPVAGITNAVPVPASKIVIAEPNGIPVWAILLVAGGLGTA
                     GFLAVSGVRKKLSERRSASCQ"
     gene            3281..4270
                     /locus_tag="PLD82_04965"
     CDS             3281..4270
                     /locus_tag="PLD82_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG64769.1"
                     /translation="MPVDGHLKELGLKIEKEQALFESCFAEFSRRVVGQRHMAERLMI
                     GLLSGGHILLEGVPGLAKTLAMRTLADIANIEFKRIQFTPDLLPSDLVGTLVFNQKTG
                     HFDTKKGPVFTNLVLADEINRAPAKVQSALLEAMQEHQVTIGETTYPLDEPFLVVATQ
                     NPIEQEGTYPLPEAQVDRFMMKVIVGYPDKDEERMIVRMHGQGSLETVRPVLGKNHIE
                     RLRKLAREVYMDDKVLDYIVGIVFASRTPAEYGLDASLFGFGASPRASLCLAAVSRAH
                     AMLQGRAFVMPDDVRAIAADVLRHRIIPSYEAEAEGITSHDLVQQVLEHVPVP"
     gene            4254..>4470
                     /locus_tag="PLD82_04970"
     CDS             4254..>4470
                     /locus_tag="PLD82_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOG64770.1"
                     /translation="MSPSHDNEDLRELLSRVREIEIRSRRMADSAMAGQYRTAFRGTG
                     IEFEEVREYASGDDVRAIDWNVTARSGK"
BASE COUNT         1054 a         1156 c         1288 g          972 t
ORIGIN      
        1 gagctgtgct gtcatgtcct ccctgcatgg tcagtcaccc gatattgcac ggggtgtcga
       61 cagttccaag gacaaagagc agggtgccgg tgaccagggc atgatgttcg ggtatgcctg
      121 caatgagaca ccagaactca tgccgctcac gatcacgctt gcccacaagc ttgtgcagga
      181 actggcgcgg atgcgcaagt ccggcgagat cgaacatatc cgtccggatt ccaagtccca
      241 ggtgaccgtc gagtatgttg acggcaagcc gaagcggatc gacgccatcg ttctttcaac
      301 gcagcatgat cccttcacgg gcgggtattc ctacaagaaa cttcgcgccg acatcgaaga
      361 gaagctcatc aaaaaagtgg ttcccaaaga attgatcgat tccaaaacga agatctatgt
      421 gaatccgacc gggatgttcg ttgttggtgg accgcatggt gattccggat tgaccggacg
      481 caagatcatc gtggatacct acggtggatt cggtgcccat ggcggtggag ctttctcggg
      541 caaggatccg tccaaggtgg accgcagtgc cgcctatatg gggcgctaca ttgcaaaaaa
      601 tatcgtcgcc tcaggtttgg ctgagcgctg tcttgtccag ttggcctacg ccattggtgt
      661 tgcagaccct gtatctgttt ttgttgatac ctatggaacc ggtaaggtca gcgatgagca
      721 gctgacggaa gccgtccggt ccgtgttcaa actcaagcca gccgagatca tccgttcgct
      781 ggatcttctc aaaccggtct acaggcagac tgcggcttat ggccatttcg ggcgtaaagg
      841 tttcagctgg gaaaaaacag acaaggctgc cgagctcaaa aagtgtttca agaagtgatg
      901 gtgtgtatgt gagcgattgc cgtggatgcg gtcctgttga ccggattccg gcagtcttgc
      961 gtgaggggga atatgatgag aggcacaaga tacctggcac tggctctcct ggtgggtatg
     1021 ttttttggtt gtaacgagcc cataccggac gcggatgtac aggcggcaaa acaggcgatc
     1081 gctcaggctg aatcggccaa ggccgataaa tacgatcccg ataatctctc aaaggcgaag
     1141 gacgcctatc gtgattctct caagcggatt gcggacagtg ataccggaaa cgcccgcgaa
     1201 gcggcccttg atgcgaagcg gatcgccgat ctggctacgg acaacgcgag gaaaaaggtt
     1261 gccgaagata ccacggccag tgccgagagg atgatccagg aggccattgt caagaaggcg
     1321 gagtatcttg ccgctgatga tcttgccaga gccagggcca agctggagga tgccaaaaaa
     1381 ggaatagccg acggaaaatg gcttgaagcc tataccgatg cggaaaactc ccttgaggct
     1441 gccggaagag ccaaggacaa tgccgtccgc aagatgtccg aacttgctaa ggccgttgcc
     1501 gatgctgaag atgctgtccg ccgtgctgat gccaacgagg ttgtcagaga gtttgcaaaa
     1561 accgagctcg acgccgccaa gcttagcttg cagaaaaccg gtgaagaaaa gaagaaggtc
     1621 gatgatccaa cgacgatcca ggctgattct gccgatacaa ggaacagact ggctgcaacc
     1681 gcctatgcgg atgccatgaa gttggcccat gaaaccgtga acaaggccaa cgatgctgtc
     1741 aggatcgcac tggagcggga acgcgaattc tacaagaaga aggccgaaca gaagatggat
     1801 gaggccaaaa aacttcttga ggatatccgc aagctcaaag aacaaggtct gatcaagcag
     1861 caggttattc cggcacccgt tgttcccgag ggagagactg tttctcagac caacattgga
     1921 atgacgaatc ttaccagtct cagtaacatg tcgaacctcg acaagtacaa cgcggctctc
     1981 gaagctctta aaaaggctga agagacctac accaacgaga attttgtcac atcggtccgg
     2041 aattcagagg aagccatccg gattgcgaaa ctggtgaagg agacggggaa gacgcctccg
     2101 gatgaactca agtactacac ggtcctcatt cctgaagcac gggattgtct atggaggatt
     2161 gccgggtatc ccttcgtcta tggcgatccc cgcctttggc caaaaatctg gaaggcgaac
     2221 aagcatctga ttgtcgatcc ggacttgatc tacccggacc agaaatttgt gataccggct
     2281 aaaaagtgat tgccgtcagg cgtgcagagg ggctgccccg aaagggcggc cccttttttt
     2341 gaaggtgcgc ccggtacccc aacgggggca gagatggggc gcatactctt gggggcggaa
     2401 ccgttactga acggcaagtc tgcccatcgt cataaaaacc gatgaattcg ctccgggttg
     2461 tctttggcaa caggaggaat atgggacgtc cggtctctgt ccacaacgtt cgggtgtatc
     2521 aactttatat ttcgggctga tggctaacag gtgcgttccg ggcattgaac gcctcctaag
     2581 gattggagat tgttcccaaa aatgaccaca aacgaattga catgcttgcc aggctgtatt
     2641 ttgcttgcta cattttttta cgggtggagt gggtgaatgc tggatgccgg ctgacagacg
     2701 ggttttggtc aggctccgca aggtacactt gtcatggaga agccatgcgt ttcctcgtgt
     2761 tgtacacact gattgccctc gcgctccgtg ctcccgctgt ccttgctgcc gcggatcccc
     2821 tttcgccgca ggtttcgtcc agtatcgacc gggatcgacc cgcactcggt gagacgttca
     2881 gcgccagtat cagggttatc ctgcctggat cggaaacgaa tgcctgcgtc atcttccctt
     2941 ccaacgttga atcacgggga atcctctccc tccagaggga agcacagact acccggtttc
     3001 tcgatggctc gaacggacag cgacgtatcg agtcaatggt ctatgtattc aagaccgaac
     3061 atgccggaag cctgtccctc gaatggccgg atgtcgtggt tttcgatccg gttgccggga
     3121 ttacaaatgc cgtaccggtt ccagcctcga aaatcgtgat tgccgaacca aacgggattc
     3181 cggtgtgggc catcctgctt gtggccggtg gtctcgggac ggccggtttt ttggcagttt
     3241 ctggagtacg caaaaaactt tccgaacgac ggagtgcatc atgccagtag acggacatct
     3301 gaaagagttg ggcttgaaaa tcgaaaagga acaagcgctg tttgaatcct gttttgccga
     3361 attttcgcgt cgggttgtcg ggcagcgtca catggccgag agactgatga ttggtctttt
     3421 gagcgggggg catattcttc tcgagggtgt gcccggtctg gcgaaaaccc tggccatgcg
     3481 aaccctggcc gatatcgcga atatcgagtt caaacgcatc cagttcacac ccgacctcct
     3541 cccttcggat cttgtcggga ctctggtttt caaccagaag acagggcatt tcgataccaa
     3601 aaagggtccg gtcttcacaa atcttgtcct ggctgacgag atcaaccgtg ccccggccaa
     3661 ggtccagtcc gccctccttg aagccatgca ggaacatcag gtcaccatcg gagaaacgac
     3721 ctatcccctt gacgaaccgt ttctcgttgt tgccacacaa aacccgatcg agcaggaagg
     3781 aacctatcct ctgcccgagg cacaggtcga tcgtttcatg atgaaggtta ttgtcgggta
     3841 tccggacaag gatgaggaac ggatgattgt ccggatgcat gggcagggca gtctcgaaac
     3901 tgtccggcct gtgctcggca agaaccatat cgagcgactc aggaaattgg cgagagaagt
     3961 ctacatggac gacaaggttc tcgactacat cgtgggaatt gtcttcgcct cgcggacgcc
     4021 tgccgaatat gggcttgatg ccagtctctt cggctttggt gcctcaccca gggcttcgct
     4081 ttgtctggcc gcggtatcca gagcccatgc catgcttcag ggcagggcct ttgtcatgcc
     4141 ggatgatgtc agggcgatcg ctgccgatgt cctcagacat cgcattatcc cttcgtacga
     4201 agctgaagcg gaagggatca ccagccatga tctcgtacaa caggtccttg aacatgtccc
     4261 cgtcccatga caacgaggat ttgcgcgagc ttctttcacg ggtacgcgag atcgagatcc
     4321 ggtcgcggcg tatggcggac agtgcgatgg ctggccagta ccggacagct tttcgcggta
     4381 ctggcatcga gttcgaggaa gtccgggagt acgcttcagg cgacgatgtc cgtgccatag
     4441 actggaatgt caccgctcgc agcggaaaac
//
LOCUS       DAOSHE010000136         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17652_length_4453_cov_3.897226, whole genome shotgun sequence.
ACCESSION   DAOSHE010000136 DAOSHE010000000
VERSION     DAOSHE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17652_length_4453_cov_3.897226"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLD82_04975"
     CDS             <1..823
                     /locus_tag="PLD82_04975"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64771.1"
                     /translation="VHAVRHALGATVRFSGVVRSGRETEIGVEQPLLHEAGGVAETGL
                     EWTRPVAGSQPDERQANREWPEVVHQKNEDAEIASSGMFAGVRIIGCYADNYWLYEQA
                     GQLYMIDQHAAHERVLYERFAAEYRSRGITRQGLLVPVQFVPGAGERDVLEGHADEIA
                     AAGFSVAPFGPKAWILDEQPADVRDGLEALRSLLAALADPAGSAARGDARLEKMLATL
                     ACHAAVRRGDRISPELVGSILQALARVDRPFSCPHGRPSIIAIKLEEIETWFARR"
     gene            805..2028
                     /gene="coaBC"
                     /locus_tag="PLD82_04980"
     CDS             805..2028
                     /gene="coaBC"
                     /locus_tag="PLD82_04980"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOG64772.1"
                     /translation="MVRTPLNVLLIATGGVAVVKAPALVRLFRKKGAVVRVVLTENAA
                     RFVTPLAFEAVSDQPVYEEMFARRDSFTHISLRQWADVVLIAPATANIMGKIAGGIAD
                     DLASTVAMGAAGAGVPRYLCPAMNDAMWAAPATQRNLAQLEKDGWEILGPASGDLACG
                     ESGPGRMLEPEEIVVAVFRKAPAVPCPGKIIVTAGATRQPIDAVRFLSNRSTGKMGVA
                     IAEAAKKVFSEVVLIHAALSVPPPVGVRVREALTAEAMLQAVTEELADAKALVMCAAV
                     SDFTPETVFSGKLKKAGRASLDIHCVPAPDILAETKALRAGNSVSTIGFAMETADLEQ
                     EAKGKLERKGLDGIVANPLDVPGAGFGTGTNLAVYFSRSGEREEWPMMSKEQMADLIL
                     TQMETLCRQKETGRE"
     gene            2036..2851
                     /gene="amrB"
                     /locus_tag="PLD82_04985"
     CDS             2036..2851
                     /gene="amrB"
                     /locus_tag="PLD82_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG64773.1"
                     /translation="MVRQPAVAGMFYPSDPAKLRSGIEGFLDRGGAVQGGLPLGIIVP
                     HAGYPYSGAVAGQAWARVRGQAPSLVVLFGPSHRVPFVGFALSESRTWETPLGKILVD
                     EESCLELEDAPCILWEAAHLEEHSIEVQLPFLQVALAGSPTILPVVCGRARPAEIQTM
                     AKAMEGLYHRRDGKVLFVCSTDLSHDYPYEQARSMDGRLASALAFCDADLLDALLARG
                     EAEACGMVGLQVMMRLARILGRARAEVLAVTNSGEVIGDTRSRIVGYLSASIP"
     gene            2871..3410
                     /gene="amrA"
                     /locus_tag="PLD82_04990"
     CDS             2871..3410
                     /gene="amrA"
                     /locus_tag="PLD82_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG64774.1"
                     /translation="MTETQRTALLQLARSSVMERLGTGELTELFPDPLWSEARGVFVT
                     LHALGRLRGCIGYIEAVEPLGQAVRTMACQAAFHDPRFRPVTADELAGIDVEISVLSP
                     LKPARAEAIVPGVHGVVIEARGRRAVFLPQVATEQGWDRETLLEELCHKAGLEGHIWR
                     EEDARLFVFTAEVFGERES"
     gene            3407..4357
                     /locus_tag="PLD82_04995"
     CDS             3407..4357
                     /locus_tag="PLD82_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOG64775.1"
                     /translation="MIYTVTLNPAIDETIELESFNAGGVSRLAETSRAVGGKGINVAR
                     ALGILGDKAEALAVVGEDSRAFFEEGARAEGIALQCFPCPGETRVSQTIVFRQECVNT
                     HIRRPHGAEGAGAALAGIKEWLASRLAQGDYLVLSGSLQTSMPPDAWSGLTELAHEKG
                     ALPVLDTSGDALEAGLAARPFMLKINLDEFMDMSEASGEEKADFKETIRSLHASGVAL
                     IVITMGAGGAVLSDGEDFFHARLEMPENRERQVWATGSGDAFLAGFLSGLLAAHPLTD
                     CLRFGVACGSVSTGYPGCARFEKEAVMETLPRVELHRELP"
BASE COUNT          794 a         1275 c         1491 g          893 t
ORIGIN      
        1 cgtacatgct gtccggcatg ccctcggggc cactgtccgc ttttccgggg tcgtccgctc
       61 cggtcgggaa acagagatcg gggtcgaaca gcctctcctg cacgaggccg gcggggtggc
      121 cgagactgga ctcgaatgga cccgaccggt cgctggttcg caacctgacg agcgacaggc
      181 gaatcgggaa tggcccgagg ttgttcatca aaaaaatgag gatgcagaaa ttgcatcttc
      241 cgggatgttc gcgggtgtcc gcatcatcgg ctgctatgcc gacaattact ggctctacga
      301 acaggccggc cagttgtaca tgatcgatca acatgccgcc catgaacggg ttctctacga
      361 gcgcttcgcg gcggagtacc gctcacgcgg gattacccgc cagggcctgt tggttccggt
      421 ccagttcgtc ccgggggctg gtgagcggga tgtccttgag ggacatgccg acgagattgc
      481 cgcggccggt ttttcggtgg cgcccttcgg tcccaaggcc tggattcttg atgaacagcc
      541 ggccgacgtc cgtgacgggc ttgaggccct gcgatccctg ctcgcggctt tggccgatcc
      601 ggccggttct gcggccaggg gcgatgcccg tctggagaag atgctggcca ccctggcctg
      661 ccatgccgcc gtgcgtcgcg gtgaccggat cagcccagaa ctcgtgggct cgattctcca
      721 ggcactggcg cgggttgacc ggcccttttc ctgcccgcat ggcaggccat ccattatcgc
      781 catcaaactt gaggagatcg agacatggtt cgcacgccgc tgaatgttct gctgattgcg
      841 acggggggag tagcggtggt caaggcgccg gcccttgtcc ggctcttccg gaaaaagggg
      901 gccgtcgtcc gtgtcgtcct gaccgaaaac gcggcccgct ttgtgacacc gctcgcgttt
      961 gaggcggtca gtgaccaacc cgtctatgaa gagatgttcg cccggcggga ctcttttacc
     1021 catatcagtc tccggcaatg ggccgatgtt gtcctgatcg caccggccac ggccaacatc
     1081 atggggaaga tcgccggggg gattgcggat gatcttgcct cgacggttgc catgggggcg
     1141 gccggtgccg gagtcccgcg gtacctctgt ccggcgatga acgatgccat gtgggctgct
     1201 ccggccacac agcgcaacct ggcgcagctt gagaaagatg gttgggagat tcttggtccg
     1261 gccagcgggg atctggcctg tggcgagagc gggccggggc ggatgctcga accggaggag
     1321 atcgtggtcg ccgtattccg caaggcgccg gccgtgccgt gtcccggaaa gatcattgtc
     1381 acagccggtg cgacccgtca gccgatcgat gctgtccggt ttttgtccaa tcgctccacc
     1441 ggaaagatgg gtgtcgccat cgcggaggcc gccaaaaagg ttttttccga agtcgtcctg
     1501 atccatgccg ccctgagtgt cccgcctccg gttggggtgc gggtccgcga agcgctcacc
     1561 gcggaagcga tgctccaggc cgtcactgag gagttggccg acgccaaggc tctcgtcatg
     1621 tgtgcggcag tttccgattt cactccggag acggtgttca gcggaaagct caaaaaagcg
     1681 ggcagggcaa gtctcgatat tcattgcgtt cctgctcccg atattctcgc cgagacgaag
     1741 gcccttcgtg ccgggaattc cgtcagcact atcgggtttg ccatggagac agcggacctc
     1801 gaacaggagg ccaagggcaa gctcgagcgg aaggggctgg acgggatagt ggctaatccc
     1861 ctggatgttc ccggggccgg ctttggaacg gggaccaatc tggcagtata cttcagtcgg
     1921 tccggagagc gcgaggaatg gcccatgatg agcaaggagc agatggcgga tttgatcctc
     1981 acacagatgg aaacgctttg tcgacagaag gaaacgggac gggagtaggg acgacatggt
     2041 acgacagccg gctgtagcag gcatgtttta tccttccgat cctgcgaaac tgcggtccgg
     2101 gattgaaggc tttctggaca gagggggtgc tgtgcagggc gggctcccgc tagggatcat
     2161 cgtcccccat gccgggtatc cctattccgg tgcggttgcc ggacaggcct gggccagggt
     2221 tcggggacag gctccgtccc ttgtggtttt gtttgggcca tcgcaccggg tgccctttgt
     2281 gggctttgcc ctttccgaaa gccgtacttg ggagacgccg cttgggaaaa tcctggtgga
     2341 cgaggagtcc tgcctggaat tggaggatgc cccctgcatt ctctgggagg cggcccatct
     2401 cgaggaacac tcgatcgagg tccagttgcc cttcctccag gtcgcgctcg caggcagccc
     2461 gaccatcctc ccggtggtct gtggtcgtgc tcgccccgca gagatacaga ctatggccaa
     2521 agccatggaa ggactctatc atcgcaggga cggaaaagtc ctctttgtct gttcgaccga
     2581 tctttcccac gattatccat acgagcaggc ccgcagcatg gacggacggc tggcctcggc
     2641 gctggcgttc tgtgacgcgg atcttctgga tgcactcctg gccagagggg aggccgaggc
     2701 ctgcgggatg gtcgggctgc aggtcatgat gcgtctggcc cggatcctcg gacgggcgcg
     2761 ggccgaggtc cttgcggtga caaattcggg ggaggtgatc ggtgataccc gctcccggat
     2821 cgtcggatac ctctcggcca gcattccgtg aacagggata ggagaacgcg atgactgaaa
     2881 cgcaacgaac ggcactcctg caactggcgc gctcatccgt gatggagcga ctcggtacgg
     2941 gggaactcac ggaacttttt ccggatccgc tctggagtga ggcgaggggt gtctttgtca
     3001 ctctgcatgc cttgggccgt ttgcgggggt gtatcggata catcgaagcg gtcgaaccac
     3061 tgggacaagc ggtcaggacg atggcctgtc aggcggcctt ccacgatccg agattccgac
     3121 cagtgaccgc ggacgagctt gccggaatcg atgttgagat atcggttctt tcacctctta
     3181 aaccggcccg tgcggaggcg attgttccag gagtccatgg cgtggtgatc gaggcccgtg
     3241 gacgccgggc ggtctttttg ccccaggtcg ccacggagca gggttgggac cgggagacat
     3301 tgctggagga actctgccac aaagccgggc tggagggcca tatctggcgt gaggaagatg
     3361 caagactctt cgtgtttacc gcggaagtct ttggagaaag ggaatcatga tctatacagt
     3421 gacactcaat ccggcgatcg acgagacaat cgagctggaa tcgttcaatg cgggcggggt
     3481 ttcccggctg gcggaaacct cgcgtgcggt cgggggaaaa ggcatcaatg tggcccgggc
     3541 tctcggtatc cttggagaca aggccgaggc tctcgctgtt gtcggagagg actccagggc
     3601 gttttttgag gaaggcgcac gtgccgaggg aatcgctctc cagtgttttc cctgtccggg
     3661 cgaaaccagg gtctcccaga ccatcgtctt cagacaggaa tgtgtcaata cccatatccg
     3721 tcgcccccat ggggccgagg gtgccggggc tgcgctcgcc gggatcaagg agtggcttgc
     3781 atccaggttg gcccagggcg attacctggt cctttcggga tcactccaga cgagcatgcc
     3841 gccggatgct tggtccgggt tgacggagct ggcccacgag aagggcgcgc ttccggtgct
     3901 cgataccagc ggagatgcgc tcgaagcagg tctggccgcc cgtcccttca tgctcaagat
     3961 caatctcgac gagtttatgg acatgagtga ggcctccgga gaggaaaagg ccgatttcaa
     4021 ggaaacgatc cggagcctgc atgcttccgg ggttgccctg atcgtcatca cgatgggggc
     4081 gggcggcgct gttctctccg atggagagga ttttttccat gcccggctcg agatgccgga
     4141 aaaccgtgaa cgacaggtct gggcgaccgg aagcggggat gcctttctgg ccggattcct
     4201 gtccggcctc ctcgccgcgc atcccctgac ggactgcctc cggttcggcg ttgcctgcgg
     4261 gagtgtcagc accgggtatc cggggtgcgc tcgcttcgag aaggaagccg tcatggaaac
     4321 ccttccccgg gttgaactgc accgcgagct gccctgatca ggcacccgcc gcgaaagctg
     4381 gatctccgga cgggacccgc gcgttgcatc attgaaaaag gtaaccgaag gtcagatgcg
     4441 ttgcctcaca ttt
//
LOCUS       DAOSHE010000137         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17883_length_4411_cov_3.738292, whole genome shotgun sequence.
ACCESSION   DAOSHE010000137 DAOSHE010000000
VERSION     DAOSHE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17883_length_4411_cov_3.738292"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLD82_05000"
     CDS             complement(<1..423)
                     /locus_tag="PLD82_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64776.1"
                     /translation="MELPGSRENYFLGGILGVATFCAIFLIGLLSGVSLGLLLLRGIL
                     FGLLVTGLGTGAGFLLERLVPGLWDDGAGLSGMGPAMDTGEGGGIDRDDQSQPSFEYT
                     VSDDVGTSEPLPDGTEDIGRTIPPEPRASRMVTGESKVV"
     gene            complement(430..1356)
                     /locus_tag="PLD82_05005"
     CDS             complement(430..1356)
                     /locus_tag="PLD82_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOG64777.1"
                     /translation="MHEEGSGGPVMDQAANLRKLASKARSTRTQPELVEVPKPAKATR
                     FIAIASGKGGVGKTNFAINLGILFAREGKRVIVMDADMGLANVNVILGVIPKFNLYHV
                     IKGQKRLPEIIIDTPQGIRIIAGASGFSKLANLTEEERLRFVDSMMELEEADIVIIDT
                     GAGVSRNVLSFVLPADEAIIVTTPEPTAITDAYGIIKAISAETGQVTIKLVVNRVSSI
                     HEGKQVAERVINIAGQFLNVRVENLGMIFQDETVPESVMKQKPFVIAAPKGRATECVD
                     HIAKRLLGMQITPKSRGFGGFIKNFLSVYKED"
     gene            complement(1349..2572)
                     /gene="flhF"
                     /locus_tag="PLD82_05010"
     CDS             complement(1349..2572)
                     /gene="flhF"
                     /locus_tag="PLD82_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOG64778.1"
                     /translation="MAYKTFTGESIKEARDAARLEFGDDFKEVDHKITVHRTGLFGLF
                     GRKEFYQITVSYDEGAPARRGGVTREMAERMELLERIKKIGDERRRAQQMQAVPVQEA
                     VSSGRPVGEDIAELKEMLATMMNTAPDAGKRKTPHHPGFSRVREFLLENDFGREYIER
                     TMEHLESSLTFRQSSDDELVRATLEKWILSSFKTGLPAINSEDSPRVITLVGPTGVGK
                     TTTLAKIGAELALGQRKRVAFITMDYYRIGAQEQLEKYAKIMNIPIYMVHSREQLEQV
                     IDEGKADFYLLDTAGRNQKNEMQIGEIRSVLQGLALPVDVHLVISATTKYADLAEIMK
                     NFATLRYEKIIATKVDETNTFGSLISSLSLCGKELVWLCMGQGVPDDIKLAEARDLAG
                     RVMVQYRIEGALSHA"
     gene            complement(2630..>4411)
                     /locus_tag="PLD82_05015"
     CDS             complement(2630..>4411)
                     /locus_tag="PLD82_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOG64779.1"
                     /translation="VIKAFGTFVVGGNYVVGLVIFMIITAVQFLVITKGATRISEVAA
                     RFRLDAMPNKYMAIDGELASGLITEEEAKNRRKVVQGETDFYGSMDGASKFVKGDVTV
                     GLLITFINIIGGIIIGTTMRGEPLSAAAGTYLLLTVGDGLVSQIPALLFSTATGIIVT
                     RSVSEGGLGEDMLNQLSSQPKVFMISVGFLAFLGFLPGFPKIPLFGLAAMLFYFGWKM
                     QQTARADERAEEEAKAAPQTHEPEVLAPVQPELFELEIGFALVPLVDESQGGDLLDRI
                     RNLRKRSANEMGIVVPPLRIRDNPRLGPNQYSIRIRSVEVGKGELQVEQFLVINPRGA
                     PDGVPGMDTRDPTFGQAARWIDREMRSTAEDLGYTVVDCPSVLATHIGEVLKSYAPEL
                     VTMQQTQIFLDSIEKTSPAIVREVKDRGARLGDIQKVLQNLLRERVSISNMERILELI
                     AEHFSAGIKHEMLTERVRQGLAMQISSTLASNGVLKVVEVDGETEELLSDAVDVDMEG
                     NMTASMDPDILNALIQNAMQTLGSVDDPEYNNIVLCTAPNRLFLQQVFEKSLPGVRVV
                     SYNELSRNIRLEILGQLGVSQKAPAWS"
BASE COUNT          921 a         1364 c         1164 g          962 t
ORIGIN      
        1 gacgaccttg ctctccccgg tgaccatcct ggatgctctc ggctcaggtg gaatcgtacg
       61 gccaatatcc tcagtcccgt ctggaagggg ttcactcgtc cccacatcat cgctcacggt
      121 atactcaaaa ctgggttgtg actgatcatc cctgtcaatc ccgccaccct caccggtgtc
      181 catcgccggg cccatcccgg acagaccggc cccatcgtcc cagagtccgg gaaccagtcg
      241 ctcaagcagg aatccggcgc ccgttcccaa acccgttaca agaagcccga agagaatccc
      301 ccgaagcagc aggagaccaa gcgagacccc tgaaagaagc ccgatcagga agatggcgca
      361 gaaagtcgcc acaccgagga tcccgcccag aaaatagttt tccctgctgc ccggtagttc
      421 cattcagcct caatcctcct tgtatacgga gaggaagttt tttatgaacc cgccaaaccc
      481 tcgcgacttg ggggtgatct gcattccgag cagacgtttg gcgatatggt cgacacactc
      541 ggttgccctg cccttgggtg ctgcaatcac aaagggtttt tgcttcatca cagactccgg
      601 aacggtttca tcctgaaaaa tcattccaag attttccacc cgtacattca agaactggcc
      661 ggcaatattg atgacgcgct cggctacctg cttcccttca tgaatggaag aaacccggtt
      721 gaccacaagc ttgatcgtca cctgtccggt ctcggccgag atggccttga tgattccgta
      781 agcatccgtg atggctgtcg gctcgggcgt tgtcacgatg atcgcctcat cggccggcag
      841 gacaaaggaa aggacattgc gcgagactcc ggccccagtg tcaatgatca cgatgtccgc
      901 ttcttcgagc tccatcatgc tgtcgacaaa acgaagacgc tcttcctcgg tcagattggc
      961 gagtttggaa aaacccgaag cgccggcaat gatccggatc ccctgcggtg tatcaatgat
     1021 gatctccggc aggcgcttct gtcccttgat gacgtgatac agattgaatt tcggaatgac
     1081 gcccaggatc acgttgacgt tggccaagcc catatcggca tccatcacga tcacacgttt
     1141 tccctcgcgg gcaaacagga ttccgagatt gatggcgaaa ttggtcttcc ccaccccgcc
     1201 ctttccgctg gcaatggcaa taaagcgggt cgcttttgcc ggcttcggaa cttcgaccag
     1261 ctcgggctgt gtccgggtac tgcgagcctt gctcgccagc tttctcagat tggctgcctg
     1321 gtccatcacc ggccctccac ttccttcctc atgcatggct gagcgccccc tcgattctgt
     1381 actggaccat gacccggccc gcaagatctc tcgcctcggc cagcttgata tcgtccggaa
     1441 caccctggcc catgcagagc cagacaagtt cctttccgca caatgagaga ctgctgatca
     1501 gggatccaaa ggtatttgtc tcgtcaacct tggtggcaat gatcttttca taccgcaagg
     1561 tggcgaaatt tttcatgatc tcggccagat cggcatactt ggtcgtagcc gaaatcacca
     1621 gatgcacatc aacgggcagg gccagaccct ggagaacgga gcggatctcg ccaatctgca
     1681 tctcgttttt ctggttcctg ccggcggtat caagaaggta aaaatcggct ttcccttcat
     1741 cgatgacctg ttccaattgc tcccggctat gaaccatgta gatggggata ttcatgatct
     1801 ttgcatactt ctcgagttgt tcctgtgcgc cgatcctgta ataatccatc gttatgaagg
     1861 ccacccgctt gcgctgaccg agagccagtt cagccccgat cttcgccagg gtcgtcgtct
     1921 tccccacgcc ggtcgggcca accagggtga tcacccgggg gctgtcttcg gaattgattg
     1981 ccggcagccc tgtcttgaac gaggagagaa tccatttttc gagagtggcc cggacaagct
     2041 cgtcgtcact gctctggcgg aaggtcagac ttgactcgag atgctccatc gtccgctcga
     2101 tatactctcg cccaaaatcg ttttcaagca ggaattcgcg cacgcgggag aatccgggat
     2161 gatggggagt ctttctcttt cccgcgtcgg gtgcggtatt catcatcgtg gccagcattt
     2221 ccttgagctc ggcaatgtcc tccccgaccg gcctgccgct tgacacggct tcctggaccg
     2281 gaaccgcctg catctgctgg gcacgcctcc tctcgtcgcc gattttcttg atccgttcga
     2341 ggagttccat cctctcggcc atctcgcgcg tcacaccacc ccgccgggcc ggagcacctt
     2401 catcatagct gaccgtgatc tggtagaact ccttgcgacc gaacaaaccg aagagtccgg
     2461 tgcgatgcac cgtgatcttg tgatccactt ccttgaaatc atcaccgaac tcgagtcggg
     2521 cagcatccct tgcctccttg atcgactcgc cggtaaatgt cttgtatgcc atgctttccc
     2581 cccatattga atatcgaaac aaacagccat cccgatgaat actcttttct tacgaccagg
     2641 ccggggcttt ttgcgagacc ccgagctgcc cgagaatctc cagccggatg ttccgggaaa
     2701 gctcgttgta cgagacaacc cggaccccgg gcagcgattt ttcgaacacc tgctgcaaaa
     2761 agagcctgtt cggcgccgta cagagaacaa tattgttgta ttcgggatca tcgaccgaac
     2821 cgagggtctg catcgcgttc tggatcaggg cgttgagtat gtccggatcc atgctggccg
     2881 tcatattccc ctccatgtcg acatcgaccg catccgaaag gagttcctcg gtctcgccat
     2941 ccacctccac gaccttgaga acgccattgc tggccaaagt actggagatc tgcattgcca
     3001 gaccctgtct gacacgctct gtcagcatct catgcttgat tcccgcactg aaatgctcgg
     3061 cgatcagctc gaggatccgt tccatgttgg agatcgacac ccgctcacgc aggagattct
     3121 gcaagacctt ctggatatcc ccgaggcggg ctccgcggtc cttgacctcg cgcacaatgg
     3181 ccggactggt cttttcgatc gaatcgagga agatctgggt ctgctgcatc gtcaccagtt
     3241 cgggggcata ggacttgagg acctccccga tatgggtcgc aaggacactc gggcaatcca
     3301 ccacggtgta cccgagatct tcggccgttg aacgcatctc cctgtcgatc cagcgggcag
     3361 cctgaccgaa ggtgggatcc cgggtatcca tcccgggcac gccatccggc gccccgcgcg
     3421 ggttgatcac gagaaactgt tcgacctgga gttcgccctt gcccacctcg acactgcgga
     3481 tacggatcga gtactggttc ggcccaagcc tgggattgtc ccggatccgc aggggcggga
     3541 caacgatccc catctcgttg gcgctccgct tgcgaagatt ccggatccgg tcaaggagat
     3601 ccccaccctg ggactcgtcc accaggggca ccagggcaaa tccgatttcc agttcaaaca
     3661 gttcgggctg gaccggcgcc aggacctccg gctcatgtgt ctggggtgct gccttggcct
     3721 cctcttcggc ccgctcatcc gcacgggccg tctgctgcat cttccatcca aaatagaaga
     3781 gcatggctgc caagccgaag agcggaatct tggggaaccc gggaaggaat ccgagaaaag
     3841 ccaaaaaacc gacggaaatc atgaagacct tgggttggct cgagagctga ttcagcatgt
     3901 cttccccgag accaccctcc gagaccgaac gggtcacaat gataccggtc gccgtcgaga
     3961 agaggagtgc cggaatctgg gagaccaggc cgtcaccgac cgtcaggagg agataggtcc
     4021 cggcggcagc cgagagtggc tctccgcgca tggtcgtccc gatgatgatc ccgccaatga
     4081 tgttgatgaa ggtgatcaaa agaccgacag tcacatcccc tttgacgaat ttggaagcac
     4141 cgtccatgga gccgtaaaag tcggtctcgc cctggacgac cttgcgccgg tttttcgctt
     4201 cttcctcggt aatcagtccg ctggccaatt cgccgtcgat cgccatgtat ttgttgggca
     4261 tggcatccag acggaaccgg gccgccactt cggagatacg ggtggcaccc ttggtgatca
     4321 caagaaactg gacggccgtg atgatcatga agatcacgag accgacaacg taattccccc
     4381 cgaccacgaa ggtcccgaag gctttgatca c
//
LOCUS       DAOSHE010000138         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_17887_length_4410_cov_4.097130, whole genome shotgun sequence.
ACCESSION   DAOSHE010000138 DAOSHE010000000
VERSION     DAOSHE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17887_length_4410_cov_4.097130"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1402)
                     /locus_tag="PLD82_05020"
     CDS             complement(140..1402)
                     /locus_tag="PLD82_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64780.1"
                     /translation="MRAAWFEVLLLLILGSAALLDADPLQNYNIYTHTPRIRIVETER
                     QIDSLWESDEQYRVVSFFSNDFRMKGSSFFIAEQSSNRMVASRGSLTRMYTNMSQVAR
                     IDLSFSRRGLNLVNLEFLHPIELPLWAESIEFWGHGHGIRHGYRVIYRDEAGQHYTLD
                     FGTTRHYGWRRFSATIPLRADTSRPFSERYKKFFITALVVLDDNASRQKPTLLHLANL
                     AVSRIRRTLFNQKEFWQFRRLVTFDAFTLPALATRFHGYTNVSVLTTRVTPSGLSKAA
                     HPLFSGLEIRADWVPEGSHRAIVRFQTPLKANVCRKIILTVKGTRRHERLWVLVQNLT
                     GSYYLLRAGQVDFDGWQKLSLDIPYWIIQQTGNITESRGLLIREILVEPAEPMMARDG
                     LSLMFGSISVVEDMGDFQAPALEILEKW"
     gene            complement(1399..3132)
                     /locus_tag="PLD82_05025"
     CDS             complement(1399..3132)
                     /locus_tag="PLD82_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64781.1"
                     /translation="MNKIPLILAILLSGSVLFSCGSKSHDQLATAGLLEYLPTKTDAL
                     VLLPSHEQAARHLFTIFVHFFGDEALRELKTKLFHNLMNETGLDLLSPGSLAENGFAI
                     DQPMAFAGTRGGGIYASIPLNDPEKAERFATGMAGAFTNSASVRMAATGQVRCILSRD
                     RLHILAGEVVAGTNQTAWTNLEGRVRSSSSTLVAVISADNSLISGGMLPVYAQPGRSL
                     ITLTRESDRILAEVTGPSIALDPIAPLRSALPFALPFGWAQIGQGIAPYFAQTDLAGL
                     SFIMNLRSLWDQTLRPFVLAQIKRSTRNLDNPLTRLLSGGKPLEERIIGMLDVENAIL
                     GNLTGRIGVVLRSLKNFSILSISPSRLQESLDGVLVFETPRAASAQAILKHLAGFSAL
                     PTGNQLEITQSDDSGVSIVTFSAPRFRMPDIHVAACGRYVLVGLSKPALSSLAAQGEV
                     RGNVNTVFGANAANLLNTTAHDVSLSIAPTTLFASITGTLDEGSRRNLEPFAAQIRKA
                     RSLALIQFRTPTGTRLLGQCILSATPEEQDPFEDTSHGSDPLLWILGSILIAVFAGPL
                     VFMIVRRFRNR"
     gene            complement(3129..>4410)
                     /locus_tag="PLD82_05030"
     CDS             complement(3129..>4410)
                     /locus_tag="PLD82_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG64782.1"
                     /translation="ATNLRRNFLRSFFSSIGIIIGIGSLYFFVTIGSGINDFIVGREM
                     QKLPMNMMRVRTTELSLGVFRFGQPDFMRSATITEKALDRFHSINGVRTIYPVMNVLF
                     PISAVLSFSSILPDQSFRRGYRTDLIMSGAPDELIREDIALTNAAFDGKAWPIPVLLS
                     RHILDIYNSGFAAGQNLPKLSEKALIGFQFDLILGQSTLLRRAGGPVTSVPCRVVGFT
                     EKAEMLGLVMPLDRVRAFNRQFVDGWTENKYSSVYILASSSDKVATIADEIEKTGFTV
                     FAEKKLSNLITLVTFILGLFSFIIIMVAAISIFNAFTVIVNQRRMEIGLFRSFGATRN
                     IIRSLFMSEAAIVGSATGLIGLVLGVFLVRGAWSLARSFLPAFLQSMESIFPVSPSLL
                     FLLFAGSVLTSLVAAWVPALVASRIEISDAVRRT"
BASE COUNT         1062 a         1107 c         1361 g          880 t
ORIGIN      
        1 actcccccac tctactatga ttccagaaac tcggcaagca cactgaaagt acgcctacgt
       61 ggatatacgt ggttcacggg gaaaatatgg cctacgatca ctgcaggggg actatggaac
      121 gcatcgggat gtcgtctaat caccactttt caaggatctc gagggccggc gcctggaaat
      181 cgcccatatc ttcgacgaca gatatggaac cgaacatcag tgacaggcca tccctggcca
      241 tcatgggttc agccggttca accagaatct cacggatcag cagaccacgg ctttccgtga
      301 tattcccggt ctgttgaatg atccagtacg gaatatccag ggagagtttt tgccagccat
      361 caaaatccac ctggccagcc cggagcaagt agtatgaccc ggtgagattc tgcaccagta
      421 cccagagtcg ctcgtgtctg cgggtacctt tgacagtcag gatgattttt cggcacacat
      481 tggctttcaa aggtgtctgg aaccggacga tggcacggtg agagccttcc gggacccaat
      541 cggcccgaat ctccaggccc gagaaaagag gatgggctgc tttcgaaagc ccagaagggg
      601 tgacccgggt agtgagcacg gagacatttg tgtatccgtg aaaccgggtg gcaagcgcag
      661 ggagtgtgaa tgcatcaaat gtgaccagtc tccggaattg ccagaattct ttctggttaa
      721 agagagttct ccggattctg ctgaccgcca gatttgccag atgcaacaat gtcggttttt
      781 gcctggatgc gttgtcatcg aggacgacga gcgctgtgat gaagaatttt ttgtatcgct
      841 ccgaaaatgg tcgactggta tctgcccgga gcgggatggt ggctgaaaac cgtctccagc
      901 cgtagtggcg tgttgtacca aagtccaggg tgtaatgctg ccccgcttca tcccggtaga
      961 tgacacggta cccatggcgg atcccgtgcc cgtgtcccca aaattcgatg ctctcggccc
     1021 agagcggcaa ctcgatggga tgaagaaact cgagattgac cagattgagc cctctccggg
     1081 aaaaggagag atcgatccgg gcgacctggg acatgttggt atacatccgt gtcagggaac
     1141 ccctgctggc caccatccga ttggaggatt gttcagcaat gaagaaagaa cttcctttca
     1201 tgcggaaatc attgctaaaa aaggagacaa cccggtactg ttcgtccgac tcccagagcg
     1261 aatcaatctg acgttcggtc tcgacgatcc ggatccgggg ggtatgtgtg tagatgttgt
     1321 aattctgcaa ggggtctgca tccagcaagg ctgcagaccc gagtatcaag agaagcagta
     1381 cctcgaacca ggctgccctc atcggtttcg gaaacgcctg acaatcatga agacgagggg
     1441 tcctgcaaaa accgcaatca ggatcgaacc gaggatccag agcaatggat cgcttccatg
     1501 ggacgtgtct tcaaacgggt cctgctcctc gggtgtggcc gagaggatgc attgcccgag
     1561 aagccgggtt cccgtcggcg tgcggaactg gatcagcgcc aacgaacgtg ccttgcgaat
     1621 ctgggctgca aagggttcga gattccggcg tgatccctca tccagagttc ctgtgatcga
     1681 agcgaacagg gttgtcgggg caatggagag actgacatcg tgtgccgtgg tattcaggag
     1741 attcgccgca ttggcgccaa agacggtatt gacgttcccc cggacttcac cctgggcagc
     1801 cagggatgag agtgccggtt ttgaaagacc gaccaggaca tagcgtccgc aggcagccac
     1861 atgaatgtcc ggcatcctga aacggggtgc ggagaatgtc acgatggaga cgccggaatc
     1921 atccgattgt gtaatctcca gctggtttcc cgtgggaagt gccgaaaaac cggcaaggtg
     1981 tttgagaatg gcctgggcgg aagctgcgcg tggtgtttca aagacgagca ccccatccag
     2041 tgattcctgg agccgggatg gcgaaatcga cagaatggaa aaattcttga gtgaacgaag
     2101 gacaactccg atgcgacctg tcaggtttcc caggatggca ttctcgacat ccagcatgcc
     2161 gatgattcgt tcctcaaggg gtttgcctcc ggagaggagc cgtgtcagcg gattgtcaag
     2221 attcctggta ctgcgcttga tctgtgcgag aacaaagggg cggagcgtct ggtcccagag
     2281 actgcgaaga ttcatgatga aggagagtcc ggccagatcg gtctgggcaa aatacggagc
     2341 gatcccctgc ccgatctggg cccatccgaa cgggagcgca aagggaagag cggagcgcag
     2401 gggggcgatc ggatcaagcg cgatggacgg gccggtcact tcggcgagta tccggtccga
     2461 ttcacgggtc agcgtgatca gggaacggcc aggttgggcg tataccggga gcataccccc
     2521 ggagatcagg gagttgtcgg ccgagatgac agcaacgagc gtggatgagg atgaacggac
     2581 acgcccttca agatttgtcc aggcagtctg attcgttccg gcaacgactt cgcctgccag
     2641 gatgtgcagc cgatcgcgac tgaggataca acggacctgc ccggtggcgg ccattcggac
     2701 ggaagcggag ttcgtaaaag ctcctgccat gcctgtggca aaacgttcag ccttttcggg
     2761 atcgttgagg ggaatggatg catagatccc cccaccccgt gttccggcaa aagccatggg
     2821 ttgatcaatg gcaaacccgt tttcggcgag tgatcccggg gagaggagat ccagaccggt
     2881 ttcgttcatc aggttgtgga agagttttgt cttgagctca cgcagggctt catcaccgaa
     2941 aaaatggaca aaaatcgtaa acagatgacg ggccgcttgt tcatgggaag gaagaagcac
     3001 gagggcatct gtctttgtcg ggagatactc gagaagcccg gcggttgcga gctggtcgtg
     3061 gcttttggac ccgcacgaga aaaggacaga tccggacagg agaatggcga ggatcaatgg
     3121 aatcttgttc atgtgcgcct cacagcatcg ctgatttcaa tccggcttgc aaccagggcg
     3181 ggaacccagg ccgcgacgag ggatgtcaga acggagccgg caaacaggag gaagaggagc
     3241 gagggagaga cagggaagat ggattccatg gattgcagga aggccggcaa aaaactccgt
     3301 gccagggacc aggccccacg gaccaaaaag acaccgagga caagcccgat caggcccgtt
     3361 gcgcttccga caatggccgc ttcactcatg aagagggacc ggatgatgtt gcgcgttgct
     3421 ccgaaggaac ggaagagtcc gatttccatc cggcgttggt tgacgatgac ggtaaaggcg
     3481 ttgaagatgc tgatggccgc aaccatgatg atgatgaacg aaaagaggcc gagaataaag
     3541 gtgacgaggg tgatcaggtt ggagagtttt ttctcggcaa agacggtaaa gccggttttt
     3601 tcgatttcgt cggcaatggt tgcgaccttg tccgaactcg aggcaaggat gtagacgctg
     3661 gagtatttgt tttcggtcca gccatcgacg aactggcggt tgaaggcgcg tacccggtca
     3721 aggggcataa ccagcccgag catctccgct ttttcggtaa acccgacaac ccggcaggga
     3781 acggatgtca ccggtccccc tgccctgcgc agcagggtcg attgtcccaa aatcaggtcg
     3841 aactggaatc cgatcaaggc cttttcggag agtttgggga ggttttgccc ggcggcgaac
     3901 cccgagttgt agatatccag gatgtggcgc gacagcagga ccgggatggg ccaggccttg
     3961 ccgtcgaaag ccgcgttggt cagtgcgata tcctcccgga tcagttcatc cggagcaccg
     4021 gacatgatca ggtccgtcct gtatccccgc cgaaagctct ggtcgggcaa gatggacgaa
     4081 aaggacagga cggccgagat cgggaagagg acgttcatca ccggatagat ggtgcggaca
     4141 ccgttgatcg aatggaaacg gtcgagggct ttttcggtga tcgtcgcgga acgcatgaaa
     4201 tccggctggc cgaagcggaa gacaccgagg gagagttcgg tggtcctgac ccgcatcatg
     4261 ttcatgggca gcttttgcat ttcacgacct acaataaaat cgttgatacc gcttccgatg
     4321 gtcacaaaaa agtacagtga cccgattccg atgatgatcc cgatgctgga aaagaaactg
     4381 cgcaaaaaat tcctgcgaag attggtggca
//
LOCUS       DAOSHE010000139         4363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18154_length_4363_cov_4.651346, whole genome shotgun sequence.
ACCESSION   DAOSHE010000139 DAOSHE010000000
VERSION     DAOSHE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18154_length_4363_cov_4.651346"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..271)
                     /locus_tag="PLD82_05035"
     CDS             complement(26..271)
                     /locus_tag="PLD82_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005384838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG64783.1"
                     /translation="MKNAPANYSRPFITGRSQAVRLPKEFRFEEDEVYLTKIDGVVMI
                     VPRDKVWAVAEGAVDRFTDDYMRSRGDDIPDARDTGA"
     gene            complement(341..1183)
                     /locus_tag="PLD82_05040"
     CDS             complement(341..1183)
                     /locus_tag="PLD82_05040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG64784.1"
                     /translation="MSGRFPRVSVCMPAFNAERTIREALLSVLGPEAVPLEVLVCDDG
                     SRDGTLTIVEAMAQADARIRVFKNEANSGISRTRNRLVREARGEYIAWLDADDLALPG
                     RLAAQMAFLDAHPEVVLLGGGIEFFADSDGQIIERRSYAGDDASLRRQIFRFSPVSQG
                     AAMVRRQALLVAGGYDEGLAQAEDLDLSFRLGRTGQFANLPQMVLRCRVHAGSVSHGR
                     MRENIRCTLRVRAKAVREYGYRMRMPDRMALVATRLALVLPTGLVRRVFVVLRRMLSG
                     GRRG"
     gene            complement(1180..2262)
                     /locus_tag="PLD82_05045"
     CDS             complement(1180..2262)
                     /locus_tag="PLD82_05045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG64785.1"
                     /translation="MSGDENRQHWDGLGRGYRKAWTTASRKRISRNETEYIRAWLPGG
                     KVHCLDIGCGTGRILEVVDAALAAGASVDAIDIAPAMLDSCRLRPWRHEARFALCDVS
                     KEAIPFVGTEWDCVTSIRVVKYNKNWEEIIEKVAGRLAPGGVFVFTMPNRRSLNIFGR
                     YAIPHYRTTRGEINRLAVRLGLEVADIRGFSRIPDVFYQVPGRLADWCLAGAETLLGW
                     ILGKRIFQREFFIVLRKPLSVGGEPAVADETSSLQKPARKKRSTPAEGRVVGKDSARK
                     TTTKTPRAETPAKKGVPAAKPAAKAVPGKQGGTGRVAQAGTKGVKGTGRPSNTKKTPD
                     TPERKTVSPKAAPKKSSGKKSGGSPR"
     gene            complement(2273..2995)
                     /locus_tag="PLD82_05050"
     CDS             complement(2273..2995)
                     /locus_tag="PLD82_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG64786.1"
                     /translation="MRYRLREISANAMPYDWPSWVYCRIGSFLLWLAQPLGLSPNAVS
                     YLGPLAGALAALSLVLWGGGDALVGKTLFILLAALAYMADEMDGIWARTTGRMSRFGG
                     WLDWKCGEAKDWLLNVGMILHWGRELLERWSPGREPFFTFEMAGLLPLFALVVLAFFA
                     GKGIFLSVRWFPEREDRDYTKKEFRLPVVCLVADAFKVSVVYSAAVFFFPVFAGWMLL
                     YTGMYWMGILVHLGKQKRSMKG"
     gene            complement(3111..3935)
                     /locus_tag="PLD82_05055"
     CDS             complement(3111..3935)
                     /locus_tag="PLD82_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOG64787.1"
                     /translation="MEAGIVAAVAFIALVLVLKGVRIVPQAENWIVERFGRYLVTLNP
                     GLNLINPLFSRVAQKVDIREGVLDLPKQGIITKDNASLQVDGIVYFKIMDPYKSYYGI
                     TNLRWAIQNIAQTTLRSIMGRMTLDESLSSREKINAELLIVLDEATDAWGTKITRVEI
                     KDIQPPEDITLAMTLQMKAEREKRARILEAEAKLEAAQREADARERLAKAEANAIQFV
                     AESLKSTGGDPLVYLLGQEYVKGLTKMSESTNGKIVVLPADILETLKSMFSSRGKG"
     gene            complement(4021..>4363)
                     /locus_tag="PLD82_05060"
     CDS             complement(4021..>4363)
                     /locus_tag="PLD82_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG64788.1"
                     /translation="LGVYADQAWQWASFFLSSLVFLGIWHAILRPRFGRKTVDETRDP
                     TVSGQTGSVVREIRPGVPGEVELSVPLYGIRRWQAMSDGILAEGTRVTVIQAEGVRLS
                     VRPVRDDQPPG"
BASE COUNT          880 a         1413 c         1259 g          811 t
ORIGIN      
        1 atattggtat cgagcaggta tccgctcatg cgccggtatc acgcgcatcc gggatgtcgt
       61 caccccgtga gcgcatgtaa tcatcggtaa agcggtcaac agcaccctcg gctacagccc
      121 agaccttgtc ccgggggacg atcatcacca ctccgtcgat cttggtcaga tagacttcgt
      181 cttcctcaaa gcgaaattcc ttgggcaggc gcacagcctg gctgcgaccg gtgataaagg
      241 gacgggaata gtttgcgggc gcgtttttca tatatcgtag tatataccag attttgctga
      301 atgtcaaggg atgtcatgac actcccggag tacaccactg tcacccccga cgcccgcccg
      361 agagcatccg ccgcaggacc acaaaaaccc ggcgcaccag tccggtcggc agcaccaggg
      421 ccagccgggt cgccacaagg gccatccggt cgggcattcg catccggtag ccgtattccc
      481 gcacggcctt tgcgcgcaca cgcagggtgc aacggatgtt ctcccgcatc cggccatgcg
      541 aaacggagcc tgcatgcacc cggcagcgca ggaccatctg cgggagattg gcaaattgtc
      601 cggtccgccc gagcctgaag gagaggtcga gatcctcggc ctgggccagc ccctcgtcat
      661 acccgcccgc aacgagcagg gcctgccgac ggaccatggc cgctccctgc gagaccggcg
      721 aaaagcggaa gatctgccgg cgcagggatg cgtcatcccc ggcatagctg cggcgttcga
      781 tgatctgtcc gtcgctgtcg gcaaaaaact cgatcccgcc gcccaagagc accacctcgg
      841 gatgtgcatc cagaaaggcc atctgcgctg ccagcctccc cggcagggcc aggtcatccg
      901 cgtccagcca ggcgatgtac tccccccgcg cctcgcgcac aagccggttg cgcgtgcgcg
      961 aaatcccgct attggcctca ttcttaaaca cacggatccg tgcatcagcc tgtgccatcg
     1021 cctcgacaat cgttagggtt ccgtcacggc tgccgtcatc acagacaagg acttcaaggg
     1081 gaacagcctc cggcccgagg acgctcaaaa gagcctcgcg gatggttcgt tccgcattga
     1141 aggccggcat gcagaccgag actcggggga accttccgct caccgcggac tcccgcccga
     1201 ttttttaccg gaagactttt tcggggcagc cttgggagac accgtctttc tttctggcgt
     1261 gtcaggcgtc tttttggtgt tcgatggacg ccccgtaccc ttgacgccct ttgttccggc
     1321 ctgtgccact cttccagtcc caccctgctt gccgggcacg gctttggcag ccggcttggc
     1381 ggcaggtacg ccctttttcg ccggcgtttc agcacgaggt gtttttgtcg tcgtttttcg
     1441 ggcggagtcc ttaccaacaa cacgcccttc cgccggggtg gagcgcttct tcctggcggg
     1501 cttttggaga gacgatgttt cgtccgccac agccggttca ccgcctaccg aaagcggttt
     1561 ccggaggacg ataaagaact cccgctgaaa aatccgtttt cccaagatcc agcccaagag
     1621 ggtctccgcc ccggcgagac accagtcggc cagccggccc ggaacctggt agaacacatc
     1681 ggggatgcgc gagaaccccc ggatgtcagc cacttccagt ccaagccgta cggccaggcg
     1741 gttgatctcc ccgcgggtgg tccggtaatg gggaatggcg tagcgcccga agatgttgag
     1801 cgagcgcctg ttgggcatgg taaacacaaa gacgccaccc ggcgcgagtc gcccggccac
     1861 cttttcaata atttcttccc agtttttgtt atacttgacg acacgaatcg aggttacgca
     1921 gtcccactcg gtccccacaa aaggtatcgc ctctttggag acatcacaga gcgcaaaccg
     1981 ggcctcatgc cgccagggtc gcagtcggca ggagtcgagc atggccggcg cgatatcgat
     2041 cgcatccacg gatgctcccg cagccagggc cgcgtcgacc acctcaagaa tccggcccgt
     2101 cccgcacccg atgtcgaggc agtggacctt ccctccggga agccaagccc ggatatactc
     2161 cgtttcgttg cgggagatcc gcttgcgcga agccgttgtc caggccttgc ggtagccccg
     2221 ccccagtccg tcccaatgct gcctgttttc gtcgccgctc atgctcacac cctcaccctt
     2281 tcatgctccg cttctgcttg ccaaggtgga cgagaatccc catccagtac atccccgtat
     2341 agaggagcat ccagcctgca aaaaccggaa agaaaaacac tgctgccgag tacacaacac
     2401 tcaccttgaa ggcgtccgct accagacaga cgaccggcag acggaattcc tttttcgtat
     2461 agtcacggtc ctcccgctcg ggaaaccagc gcaccgacag aaagatgccc tttccggcaa
     2521 aaaaggccag caccacgagg gcgaagagcg ggagcaggcc cgccatctcg aacgtgaaaa
     2581 acggctcccg tccgggtgac catcgttcca acaactcccg cccccagtgc aggatcatcc
     2641 ccacgttcaa aagccagtcc ttggcctcac cgcacttcca gtccagccag ccgccaaagc
     2701 ggctcatccg ccccgtcgtc cgggcccaga tcccgtccat ctcgtcggcc atataggcca
     2761 gggccgccag gaggataaag agggttttgc cgacaagggc gtctccgccc ccccagagga
     2821 cgagggagag cgcggccagc gcgcccgcaa ggggcccgag gtatgagaca gcgttgggag
     2881 agagcccgag tggctgggcc agccagagca aaaatgagcc gatacggcaa tagacccagg
     2941 agggccagtc gtagggcatg gcattggcag aaatctcgcg cagtctgtac cgcatgaaga
     3001 tcctcacgct tgacgttggc ggggacaagg gaagctccct gaccccataa aaaggaccgc
     3061 ccccggatag aagcggtctc attgaatata ctatccctga gcgatgccgg tcagcccttg
     3121 cctcttgacg agaacatgga cttgagggtc tcgaggatat cggccgggag tacaacgatc
     3181 ttgccgttgg ttgactcgct catcttggtc aggcccttga cgtattcctg cccgaggaga
     3241 tagaccaggg gatcgccccc ggttgacttg agggattcgg ccacaaactg gatggcgttg
     3301 gcctcggcct tggccaggcg ttcacgggca tccgcttcac gctgggcagc ctcgagcttg
     3361 gcctcggcct cgaggatccg ggcgcgcttc tcgcgctcgg ccttcatctg gagggtcatc
     3421 gccagggtga tgtcctcggg cggctggata tccttgatct cgacacgggt gatcttggtc
     3481 ccccatgcat cggtcgcctc gtccaggacg atcaggagtt cggcattgat cttctcgcgc
     3541 gaggagagcg actcgtccag ggtcatccgg cccatgatcg aacggagcgt ggtctgggcg
     3601 atattctgga tggcccagcg caggttcgtg ataccgtaat atgacttgta cggatccatg
     3661 attttgaaat agacaatccc gtccacctgg agtgaggcgt tgtccttggt gatgatcccc
     3721 tgtttgggca ggtcaagcac cccttcgcgg atgtccacct tctgcgccac acgggaaaag
     3781 agcgggttga tcaggttgag ccccggattg agggtgacga gatagcggcc aaagcgttcg
     3841 acaatccagt tttcggcttg ggggacaatc ctgacaccct tcaagaccag gaccagggcg
     3901 ataaaggcca ctgctgcgac aattccagct tccatatata ttcctccttc tatcgggata
     3961 ccggtttttc cggatatcac aatttttgct gaacaacctc aggcaggatg caaacggcag
     4021 tcacccgggg ggttggtcat cccgcacagg ccggaccgag agacggaccc cctcggcttg
     4081 aatcacggtc acacgggttc cctccgccag aatcccgtca gacatggcct gccagcgccg
     4141 gatcccgtac agcgggacgg aaagctcgac ctccccgggg acccccggcc ggatctcgcg
     4201 gacaacactc ccggtctgcc cgctgactgt aggatcccgg gtctcgtcaa ccgtcttgcg
     4261 gccaaagcgc gggcgaagga tggcatgcca gatccccaga aagaccagcg acgagaggaa
     4321 gaaggaggcc cactgccagg cctgatcggc atacacaccc aag
//
LOCUS       DAOSHE010000140         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18416_length_4317_cov_3.893477, whole genome shotgun sequence.
ACCESSION   DAOSHE010000140 DAOSHE010000000
VERSION     DAOSHE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18416_length_4317_cov_3.893477"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLD82_05065"
     CDS             <1..653
                     /locus_tag="PLD82_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOG64789.1"
                     /translation="VMIFAVKYPQGAEKQLIKAATGLEVPSGGLPMDVGVVVSNAGSL
                     IALKEAIVDDKPLFERVVTVTGDAVQKPGNYKIVVGARVADILAEVGLKKKPARIILG
                     GPMMGLAQYTDQVPVTKGTSGIMVLSRRRSAIYRHRPCVRCGKCIDVCPMGLMPLKLV
                     TQIELVQDEAAKKLGMLDCIECGCCSAVCPSERHLAQTIKLGKLRHIAKKKEAAGD"
     gene            646..1698
                     /locus_tag="PLD82_05070"
     CDS             646..1698
                     /locus_tag="PLD82_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG64790.1"
                     /translation="MIKYSSEDLVVSSGPHLRTKLRVPVVMWLVSTTLIPAIAWAGYA
                     FGPWVLIVTAVSVLAAVLTEFVITLLLGKPQTVTDGSAFLTGLLVACNMPATEWGVLP
                     LYVPIVGSIFAIAVAKHAFGGLGQNWINPALAGRIFVFFAWLPAMTGGWAGSPHFDAV
                     SSATPLNMLKFRTGILPGDIQTTSDAVLGMHLSPTHGDLFFGMVPGCIGEISALALLV
                     GGLILIVLKIVQWEIPLFYIASTALFSWIFGGLAVAGQPQAFFSGDALYHILSGGVIL
                     GAFYMATDWVTTPVSFGGRIIFGLGCGALTVLIRLTGSNVEGVSFAIVLMNITVPLID
                     RLFSPQRFGLPEGRGV"
     gene            1695..2747
                     /locus_tag="PLD82_05075"
     CDS             1695..2747
                     /locus_tag="PLD82_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG64791.1"
                     /translation="MKQFLKTAGILAGVCFVAALLLSVTDRLTQKAIAETGQKKKDAA
                     RQTVLQAARYTTYAIPDSSEVTNFLLRYSERTGIRFVPCRSLVQSNYSEARDASGKLV
                     GYVFDVRAPGGYGGNLDIIVGVRNSATGKLAVSDYLVIASQETPGLGKAVEISMHSAF
                     TNPGRPQGLGLLDPRDRRSDCVSGATITSVAIKDATYAALRMAVVLEERNFVRLDIPE
                     GEMAIVPYSQTYEPVNLPFNETVRECLDVRWYGQTSGYLLKIVLKDAETRKKYLALAG
                     FTAIDGKLHGTRLYTMPLSTNGVFTRVPDFNEALFSATEAASLATNAGLGRLEKELAG
                     SIAAGRAMLGKAGKLP"
     gene            2744..3343
                     /locus_tag="PLD82_05080"
                     /pseudo
     CDS             2744..3343
                     /locus_tag="PLD82_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
     gene            3442..4029
                     /gene="rsxA"
                     /locus_tag="PLD82_05085"
     CDS             3442..4029
                     /gene="rsxA"
                     /locus_tag="PLD82_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG64792.1"
                     /translation="MSVEAVILLVMSAIFINNFVLAKFLGICPFIGVSRQLDSALGMG
                     MAVTFVLTISSVATWCVYQYLLVPYGLEFLKTIAFILVIAALVQFVELVLKKTSPVLY
                     KTLGIYLPLITTNCAVLGAASINVQKGYNLLMSTVFGLGAGLGFTLALILMAGIRERL
                     EECNIPRVLRGAPIAFITAGLISLAFMGFAGMIKE"
     gene            4097..>4317
                     /locus_tag="PLD82_05090"
     CDS             4097..>4317
                     /locus_tag="PLD82_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG64793.1"
                     /translation="METIIAAVLVISGLGFLLGYGLAFAAKKLETPRDERAAAVEQCL
                     PGVNCGACGYAGCSGYAQAIVTQGVPLHL"
BASE COUNT          850 a         1161 c         1223 g         1083 t
ORIGIN      
        1 aggtgatgat atttgcggtc aagtatcccc aaggtgccga aaagcagctc atcaaggcag
       61 cgaccggtct cgaggttccg tccggtggtc tgcccatgga cgtcggggtt gttgtctcga
      121 atgccggttc cctgattgcc cttaaagaag caatcgttga cgacaaaccc ctgtttgaga
      181 gagtggtcac cgtgacaggg gatgcggtcc agaaaccggg aaattacaag attgttgtgg
      241 gcgccagggt tgccgacatt ctggccgagg tcggcctcaa aaagaaacca gccaggatca
      301 ttcttggcgg tccgatgatg ggacttgccc agtatacgga ccaggttccg gttaccaagg
      361 gaaccagtgg aatcatggtt ctctccaggc gcagatccgc catataccgg caccgcccct
      421 gtgtccggtg cgggaaatgc atcgatgtct gccccatggg tctgatgcct ctcaagcttg
      481 taacccagat tgaactcgtc caggacgaag ctgcgaaaaa gctcggtatg ctggattgta
      541 tcgaatgcgg gtgttgttcg gccgtctgtc cttccgaacg gcacttggcc cagacgatca
      601 agcttgggaa gctgcgccat atcgcaaaaa agaaggaggc ggcaggtgat taagtactcg
      661 agcgaggacc ttgtcgtctc ttcgggaccg catctcagga cgaaactgcg tgttcccgtc
      721 gtgatgtggc tggtctcgac aaccctgatt ccggccatcg cctgggccgg gtatgctttt
      781 ggtccctggg tcctgattgt gaccgcagtc tccgtccttg cggccgtttt gaccgagttt
      841 gtgatcacgc tccttctggg caagccccaa acagtcacgg acggttccgc ctttctgacg
      901 ggactcctgg ttgcatgcaa tatgcccgcg acggaatggg gtgttctccc tctttatgtc
      961 ccaatcgttg gcagcatttt cgcgattgcc gtcgccaaac atgcctttgg cggactaggg
     1021 cagaactgga tcaatccggc attggcggga cgaattttcg tcttctttgc ctggctcccc
     1081 gcaatgacag gtggctgggc gggatcgccg cactttgatg cggtgtcgtc tgcgacaccg
     1141 ctcaatatgc tcaagttccg gaccgggatt ctccccggag acatccagac caccagtgat
     1201 gccgttcttg gaatgcatct ttcgccgacc catggggacc tttttttcgg gatggttccg
     1261 gggtgtatcg gagagatttc cgcgctggcc ctgctggtcg gtggactgat cctgattgtt
     1321 ctcaagatcg tccaatggga gatccccctg ttctacattg cctctacagc gctcttctca
     1381 tggatttttg gcggcttggc tgtagcagga cagccccagg ctttctttag cggagacgcg
     1441 ctctaccata tcctctcggg cggcgtaata cttggagctt tttacatggc gaccgattgg
     1501 gtgacgacgc cggtttcctt cggaggacgg atcatctttg gtctggggtg tggtgccttg
     1561 acagtcttga tacgcttgac cggatcgaat gtggaaggtg tatcctttgc cattgttttg
     1621 atgaacatca ctgttcccct gatagaccgg ttgttttcac cccaaaggtt tggccttcct
     1681 gaggggaggg gtgtatgaaa caatttctca agactgccgg aatcctggct ggcgtctgtt
     1741 ttgtggcagc cctcctgctt tcggtaacgg accggttgac acaaaaggcg attgccgaga
     1801 ccggccaaaa gaaaaaggat gcggccaggc agaccgtgtt gcaggcggcc cggtacacga
     1861 cgtacgccat cccggacagc agcgaagtca ccaatttcct cttgcgatac agcgaacgga
     1921 ccggaatccg gtttgtgcct tgtcgttctc tggttcaaag caattattcc gaagccagag
     1981 acgcttcagg gaaacttgtc gggtatgttt tcgatgtccg tgctcctggc gggtatggcg
     2041 ggaatctgga tatcatcgtc ggagtccgaa actctgcaac cggaaagctt gccgtcagtg
     2101 actatcttgt gatcgccagt caggaaacac cgggtctggg caaggctgtc gagatatcca
     2161 tgcactctgc tttcaccaat ccgggacgac cacagggact gggcctcctg gatccacgtg
     2221 atcgtcggtc agattgtgtc agtggcgcga ccatcacatc ggttgccatc aaggatgcaa
     2281 cctatgctgc actccgcatg gctgtcgttc ttgaagagcg caattttgtc aggctggaca
     2341 ttcccgaagg cgaaatggcc atcgtaccgt actcccaaac ctacgagccg gtcaatctgc
     2401 ctttcaacga gaccgtccgt gagtgtctgg atgtgcgctg gtacggtcag acgagcgggt
     2461 acctactcaa gatcgtcctg aaggatgcgg agacacgaaa aaaatatctg gctctggccg
     2521 gttttaccgc gattgacgga aaactgcacg ggacgaggct ctacaccatg ccgctctcga
     2581 ccaatggcgt ctttacccgg gtgccggatt tcaacgaagc gttgttttcc gcaacagagg
     2641 cagccagtct tgcgaccaat gccgggctgg gccggcttga aaaagaactg gctggttcca
     2701 tcgctgccgg aagggccatg ctcggaaagg cggggaaact cccatgaacc agattcgaaa
     2761 aggaatcttc aaggaaaatc cggttttcgt catcatgctc gggctctgtc cctcactggc
     2821 ggtcagtacc caggtgatca acggaatcgg gatgggtgtc gcgattcttg tcgtgttgct
     2881 cggttccaat cttgtcatct ccctgatgcg ctccatggtt cccgatacga tccggattcc
     2941 ggttttcatc gtggtcattg cctcctttgt caccatcgtc gacctggcga tgcaggcctg
     3001 gctgcccagc ctcgcggacg cactgggaat cttcattccg ctgattgtcg tcaactgcat
     3061 catcctggga agggctgagg cctttgcctc caaaaattcg ccgtttccgt ccctgctgga
     3121 cgcgatcgga atgtcaacgg gtttcatgct ctccctgctt ctgctggcaa ccatccgcga
     3181 gcttctcggg aatggaacga tgaccctgga gattctcggt atcgggacga caatccatgt
     3241 tccttttctt gaggagcaca aggctgtgat catgatcctt cctccgggcg cgttttttac
     3301 gatgggactc ctgatgggtc tctttaatct gatccggaag aaatcgacgg gaaccgcaaa
     3361 taccctgacc tggcgtgatg cgcatatttc acaccgacgc agggatatct gttgtggtgg
     3421 actggtcggg aggaaaaaca catgagtgtc gaagccgtca ttctgctcgt gatgagcgca
     3481 atcttcatca acaattttgt cctggccaaa tttctcggga tctgcccctt tatcggggta
     3541 tcccgccagc tggattccgc gctggggatg gggatggccg taacctttgt cctgacgatt
     3601 tcctcggtgg caacctggtg cgtctaccaa tacctgctcg ttccctatgg ccttgaattc
     3661 ctcaagacga tcgccttcat tcttgtgatt gcggctctgg tccaatttgt cgaactggtt
     3721 ctcaaaaaga caagcccggt tctatacaaa acattgggaa tctacctgcc cttgatcacc
     3781 acaaattgcg ctgtgttggg tgctgcatcc atcaatgtcc aaaagggcta taacctcctg
     3841 atgagtacgg tttttggact tggtgccggt ctcgggttta cccttgccct gatcctgatg
     3901 gccgggattc gtgaacggct tgaggaatgc aatattccca gggttctcag gggtgccccg
     3961 atcgccttca tcacggccgg tctgatttct ctggcgttca tgggatttgc agggatgatc
     4021 aaggaatgag gacggatttg ccaggcagac cctgcctgtc agcgtatggg tcgcatcctt
     4081 gcaaggagtg taactgatgg aaacgattat tgcggcggtt cttgtgattt ctggactcgg
     4141 gtttcttctc ggatatgggc ttgcttttgc cgcaaaaaaa cttgaaacac cgcgtgatga
     4201 acgcgctgct gccgtcgaac aatgccttcc cggagtcaat tgcggtgcct gcgggtatgc
     4261 cggctgttcg ggctatgcgc aggccattgt cacccagggc gttcccctgc atttgtg
//
LOCUS       DAOSHE010000141         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18568_length_4289_cov_3.581719, whole genome shotgun sequence.
ACCESSION   DAOSHE010000141 DAOSHE010000000
VERSION     DAOSHE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18568_length_4289_cov_3.581719"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PLD82_05095"
     CDS             complement(<1..1721)
                     /locus_tag="PLD82_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64794.1"
                     /translation="MRFCLNSFCFLFLLSACASSTGLTREGDHLSDLLFQADAGKAYR
                     LAVNRNQENPHALTGLASWYLRRGEAGKAAFLLSSACAFPDAPAKSWELNARLLQRTG
                     RTDAANLALSRGLGLYPDDPGLLRARTELARTRTIAAARSRLATQPGDKNARQELCYA
                     LLDSGWTTLALNEATILVQQGQADAQFLMAIVRTLEHQGKHDYLTVWLEHLLAADYRN
                     AHALERLSRIAIDRDDLWRANSLISTYLMHYPDDGMMLERGGHLSIRLGKHDEGKRWL
                     EKAWSSGRKTHTIAETLGHIALNHSKDQAGAEAWFERARQLQPDTSSATLSIRRRRLE
                     NQRLQHFADFYGQDAQRQATILLRMAENSLEIGEYRTALDLATRVTETQPNQADGFII
                     KARAQMALTRFERSRTSLKRALQLEPANPRAWWYLAELHANQALPDPDARLDALRQAV
                     RLAPGEPVYTEAMAAFWLSRNNLDEALNWYRKASSLNRSSTNRETLRSLERRLIERDL
                     KADESSAQGNRDALIETGRRYFALLGPAHPGYHRCLEAASLLDPEHAGLHVLTADAAI
                     KLFLSDM"
     gene            complement(1773..2888)
                     /locus_tag="PLD82_05100"
     CDS             complement(1773..2888)
                     /locus_tag="PLD82_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG64795.1"
                     /translation="MTLIGFLGILGGVAVAGIVAYQIIIALKYKGMRYNWFTFKHEGF
                     SYGLEKPDILLLRDIAFENRVPDFMMMYSSQRTLDTSVIRSVERVRNSDTTDEEKHTS
                     IERIFLLRNKMDGIIASRKQTISHSNQIRANQPVELTFERIGTYQTYVLESTPKFFAA
                     AMPIEALDVQDFTWEGKKVRVKFHVSNDAEYAFISKVLDQTMGSGITGHINIEHSNRI
                     TRTQKRIFRRNSVSIAVSMFSLRVTGEGASRKVSIANNIPFSGTILNLSAGGVAVRAG
                     GLLRENTLVKLDFSLDFENTEVAIGRVLACTSIPNSTERMLHIRFERISRKTRNNIFE
                     YIYREADEKKNFAPKTIIPTANGILLSPDGTSTGNGE"
     gene            3101..>4289
                     /locus_tag="PLD82_05105"
     CDS             3101..>4289
                     /locus_tag="PLD82_05105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG64796.1"
                     /translation="MFTVLHVESSHFFTKLYRRIVEGQKARYLEAGSADEALQILEKE
                     HVDIIISASTVDGGLDSFVKRLGQSRFRILPVVVSSATDTAEERTRMFALGVVDYIVK
                     EDSDEHLARFIEKMVQADSRMDALKSLSIAVLDDSETVHEVMRRTFAIYGIVSMKSYY
                     SAQEILAAPDHDIYLVDINLPDMSGTQIVLEVRKRAPDAVIIAVSAIDHYKVISNTLI
                     AGADDYIIKPFNAMILMARLQASARMLLLYRELEEKSCRLEELVVRDSLTGLFNHKHI
                     IDRLAEEVEKATRYHHKLSVVMFDIDHFKAVNDTWGHQIGDQVLVEFARLLMETARNC
                     DIVGRYGGEEFFVILPETDLDAAMVFGNRLRERLEETRFPGMPELVVTVSGGAAEWKG
                     GGHL"
BASE COUNT          814 a         1129 c         1389 g          957 t
ORIGIN      
        1 tgcatgtcgg agaggaagag tttgatggcc gcgtcggccg tcaggacgtg caatccggcg
       61 tgttccggat cgagcaggga tgccgcctcg agacagcgat gatatccggg gtgagccggg
      121 ccgaggaggg cgaagtagcg ccggccggtt tcgatgaggg catcccggtt tccttgtgct
      181 gacgactcgt ccgctttcag atcccgctcg atcaggcgtc gttcaagact gcgcagggtt
      241 tcgcgatttg tgctggagcg gttcagactg gaagccttgc ggtaccagtt gagtgcctcg
      301 tcaagattgt tccgtgagag ccagaaggcg gccatggcct cggtgtagac cggctccccg
      361 ggtgcaagac ggacggcctg cctgagcgca tccagccgcg catccggatc cggcagggct
      421 tgattggcat ggagttcggc gagataccac caggccctcg ggtttgccgg ttcaagttga
      481 agagcgcgtt tgagcgaggt ccggctgcgt tcaaagcgcg tcagtgccat ttgggcgcgg
      541 gccttgatga taaatccgtc cgcctgattg ggctgcgtct cggtgacgcg tgtggccaga
      601 tcgagggccg tccggtattc cccgatttcg agagagttct cggccatgcg caggaggatg
      661 gttgcttgcc gctgggcatc ctgtccataa aaatcggcaa agtgttggag tcgctgattc
      721 tcgagccgtc tccggcggat ggagagggtg gccgaactcg tgtcgggctg gagttgtctt
      781 gcccgttcga accaggcctc ggccccggcc tggtccttgc tgtggttgag ggcgatgtgg
      841 ccaagggttt cagcgatggt atgcgttttg cggccgctgg accaggcctt ctccagccag
      901 cgcttgccct cgtcgtgttt gcccagacgg atggagaggt ggccgccccg ttcaagcatc
      961 atgccgtcat cgggatagtg catgaggtag gtgctgatca gggagttcgc tctccagaga
     1021 tcatcccgat cgattgcaat ccgggagagc cgttcgaggg catgggcatt ccggtaatcg
     1081 gccgcgagaa ggtgttcgag ccagactgtc aggtagtcgt gtttcccctg gtgttccagt
     1141 gtgcggacga tggccatcag gaattgcgcg tcggcctgcc cctgctggac gaggatggtt
     1201 gcctcgttca gggcgagcgt tgtccagccg gagtccaaca gggcgtagca caattcttgg
     1261 cgggcgtttt tgtcacctgg ttgtgtcgcc aggcgggagc gtgctgccgc gatggtccgg
     1321 gtgcgggcca gttcggtccg ggcccggagg aggcccggat catccggata caggccgaga
     1381 ccgcgggaga gtgcgaggtt tgcggcatct gtccggccgg tccgttgcag gagtctggca
     1441 ttgagttccc agcttttggc cggagcatcg ggaaaggcgc aggctgacga gagaagaaat
     1501 gcggcttttc ccgcttctcc ccggcggaga taccaggagg ccagcccggt cagagcatgg
     1561 ggattctcct ggttccggtt gacggcgagg cggtaggcct tgcctgcgtc agcctggaag
     1621 agcaggtcgc tcaggtggtc gccctctcgt gtgaggcccg tgctggatgc gcaggccgag
     1681 agaagaaaca gaaaacagaa agagttcaaa caaaagcgca tgccggatcc cgctcagata
     1741 agtatgcaac aataatactc aaggggaggg ggttattctc cgttgccggt gcttgttccg
     1801 tcaggactga ggagaatccc gttggcggtg gggatgatcg ttttcggggc aaaattcttt
     1861 ttttcatcgg cctcgcggta gatgtattcg aagatattgt tccgggtctt ccggctgatg
     1921 cgttcaaacc ggatatggag catccgctcg gtgctgttgg gaatcgatgt gcaggccaga
     1981 acccgaccga tggcgacctc cgtgttctcg aaatcgaggc tgaagtccag cttgaccaag
     2041 gtgttttccc tgaggagtcc gccggcccta acggccacac cgcccgcgct caggttgaga
     2101 atcgttccgc tgaaggggat gttgttggcg atggaaacct tgcgggatgc accctctccg
     2161 gtcacccgga gggagaacat gctgaccgcg atagaaaccg aattgcggcg gaagatgcgt
     2221 ttttgggtcc gggtgatacg gttgctgtgc tcgatattga tgtgtccggt tatcccgctt
     2281 cccatggtct ggtcgagaac cttgctgatg aaggcgtatt cggcgtcgtt gctgacgtga
     2341 aacttgaccc tgaccttctt gccctcccat gtgaaatcct ggacatccag ggcttcgatc
     2401 ggcatggccg cggcgaagaa tttgggcgtg gactcgagga cgtaggtctg gtaggtgccg
     2461 atccgttcaa acgtcagttc gaccggctgg tttgcccgga tctggtttga atggctgatg
     2521 gtctgcttgc ggctggcgat gatcccgtcc atcttgttgc gaaggaggaa gatccgctcg
     2581 atggaggtgt gtttctcctc gtcggtggtg tccgaattcc ggacgcgctc tacgctccgg
     2641 atgaccgagg tgtcgagggt gcgttgcgag gagtacatca tcatgaagtc aggaacgcgg
     2701 ttttcaaagg caatatctcg aaggagaagg atgtccggct tctcgagtcc gtaggaaaaa
     2761 ccctcatgtt tgaaggtgaa ccagttgtag cgcatccctt tgtatttgag ggcaatgatg
     2821 atctggtagg caacaatgcc cgcgacggcc actcctccca gaatccccag aaaaccgatg
     2881 agtgtcattg ccatctccat tcagggtact tcgcctgatg cgaagctgtg tcctttatag
     2941 ggacactctt ccgtatatcg gcgcttgccc gggaaaaatt gatactccgg aggataatcg
     3001 tcgcccaaat tggatacgct gcatcattga accgggagat attgacgaaa gccggaaatt
     3061 gtgtgataca ctcaggagtg aagccgaacg gaggtccccc atgtttacgg tgttgcacgt
     3121 ggaatcgagt catttcttca ccaagctcta ccgcaggatt gttgaggggc agaaggcccg
     3181 ctatctcgag gccggttcgg cggatgaggc actgcagatt ctcgagaagg agcatgttga
     3241 tatcatcatc tccgccagta cggtcgatgg cgggctggat tccttcgtca agcgccttgg
     3301 ccagagccgc ttcaggatcc tccccgttgt tgtttccagc gcaaccgata ctgccgagga
     3361 gcggaccagg atgttcgcct tgggggtggt cgactacatc gtcaaagagg attcggatga
     3421 gcacctcgcg cgttttatcg agaagatggt ccaggccgac agccggatgg atgccctcaa
     3481 gtcgctctcc atcgcggttc tggatgattc cgaaaccgtg cacgaagtga tgcgccgaac
     3541 ctttgcaatc tacgggatcg tttccatgaa gtcctattac tcggcacagg agatcctcgc
     3601 tgcgcctgac catgacatct atctggtgga tatcaatctt cccgacatgt ccggaaccca
     3661 gatcgtcctc gaggtccgca agcgtgctcc ggatgcggtc atcatcgccg tctccgccat
     3721 cgaccactat aaggtgattt cgaacaccct gattgccggg gcggatgatt acatcatcaa
     3781 accctttaat gcgatgatcc tgatggcccg tctccaggcc agcgcacgga tgcttctcct
     3841 ctaccgtgaa ctcgaggaga aaagctgcag gcttgaggag ctcgtcgtgc gggacagctt
     3901 gacggggctg ttcaaccaca agcacatcat cgaccggctg gccgaggaag tcgagaaggc
     3961 gacccgctac catcacaagc tttcagtggt catgttcgat atcgatcact tcaaggcggt
     4021 caacgacacg tggggtcacc agatcggaga ccaggtgctc gtggagttcg ccaggctgct
     4081 gatggagacg gcacggaatt gtgatatcgt gggccggtat ggcggagagg aattttttgt
     4141 catcctcccg gagaccgatc tcgatgcggc catggttttc gggaaccggc tccgggagcg
     4201 tctcgaggaa acgcgtttcc ctgggatgcc cgaactggtg gtaacggtct cgggtggtgc
     4261 ggccgaatgg aagggcggcg ggcatctga
//
LOCUS       DAOSHE010000142         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18618_length_4280_cov_3.598580, whole genome shotgun sequence.
ACCESSION   DAOSHE010000142 DAOSHE010000000
VERSION     DAOSHE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18618_length_4280_cov_3.598580"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLD82_05110"
     CDS             complement(<1..460)
                     /locus_tag="PLD82_05110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOG64797.1"
                     /translation="MQNYRYDLPMMIMMLEEEELLDDFVRDAFTFDILCSQNFEITDI
                     LFNPLKSIGEKLEWVDEMLIIYFSDAYLSFLRQLIKNEDFIYYERLRAKFLATLSRMR
                     NCLYAKVTTVIPMTEEQSKRVAAKLRELFGKQVFVYNKVSRYFSGGLLIEC"
     gene            complement(480..974)
                     /gene="atpF"
                     /locus_tag="PLD82_05115"
     CDS             complement(480..974)
                     /gene="atpF"
                     /locus_tag="PLD82_05115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOG64798.1"
                     /translation="MFTFEPGVAIWTLISFVIVLIVLSKFVLPPITKAIDERKNKISS
                     DLEAADEAARKAERTSSDLTRRLAAIDREREELLAEARERARSRFEELEKETHAAMNE
                     MRKQREAELAAESEQFLALTGHRINQLIISGCERVLRTGLTSEQQAQVLENRIREFEK
                     MTRV"
     gene            complement(1020..1316)
                     /gene="atpE"
                     /locus_tag="PLD82_05120"
     CDS             complement(1020..1316)
                     /gene="atpE"
                     /locus_tag="PLD82_05120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG64799.1"
                     /translation="MDSLGAAIAISIAALGGTLGIGLMMSRAFESIARQPEITSRIRP
                     LIFVGIAFIEAAVLYALVIAIMLASKTPQPTAQAAAHGARPAVTSTVSNQTPQP"
     gene            complement(1329..2150)
                     /gene="atpB"
                     /locus_tag="PLD82_05125"
     CDS             complement(1329..2150)
                     /gene="atpB"
                     /locus_tag="PLD82_05125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG64800.1"
                     /translation="MKKDSPSHRRPRSGPDLTGSDTTASAEEAGGYSTMHHTPTVLVP
                     IHIGGIDLSITAAVLWTFISVALVLVFGFLARRRVTRIPHRLSQNLFEITIEFVRSQI
                     LEPNELDTRRWTPLFLSVFLFIFFNDLANVIPGATSGSGNLNETFALAFVFFTLAVAL
                     RFKVHGPFGFWKSLIPSGVSGPVVALMFPIELVSQLFKPISLAVRLFANMSGGHALFL
                     TIIAFAASATNAVVPVFSVFGGIVILLFELFVSFIQAYVFAFLSALMVAEAEQKH"
     gene            2337..>4280
                     /locus_tag="PLD82_05130"
     CDS             2337..>4280
                     /locus_tag="PLD82_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOG64801.1"
                     /translation="MAAKDRPSFKLGYTCVTPGVAALLPPALIMELFTRHEARDWGAI
                     QPEDALENDRNADSGGRVRSVYLVSPDRIRQAGGVPAPASENDAMPDDAVEVWIITEP
                     DRFDSILMLPGEYIAWESGEKRVYRERLHAALQATPGHVSASAAPGDDKSQTGQRPLP
                     GEDFFSWLEEFFTAVYWTRYREQRFERRFAKGMLVPGLDRMTDDGLEKNIDEFRRELV
                     KTIRDGTFRFRFLRRIDLDEEGKRRTVALPVMRDRLLDNILARFLSNRLGLRPQAHVL
                     VTSRHVQKLITGGHASWFIRTDIEGFFDSIDHTKIRAWLEKQGLQQEVGRLVSELAIT
                     PRLTPEEMTLFRTENTRTGWEQAVRSAMTPVRGLPQGLYTSNILAEAWLADWIARHPL
                     PKRTALVRYVDDFLFLAERWSGLRALKRYMKRMAKDLSLGFSAGKTVSGRVRDGFDFL
                     GFFHRPGYARRSDRSVRRFKMRWIEALENPMRFLESRMDPWYLHGLIDTTDDAMLRES
                     VIFGLVVYYYNLSIIGWGRTWEPANLLEYDNLQQVLPQIVEGQVEIEEGEADTLRERF
                     AELGRDLFEITISGSALYNSFCTDFRQIDDLNRWAGRLFSGYAARKGLTKPVLEDHLK
                     WYFYFRKHRTGYNRKVLREYIRLY"
BASE COUNT          908 a         1147 c         1311 g          914 t
ORIGIN      
        1 cgcactcgat caggagcccc ccggagaaat agcgggagac cttgttatag acgaagacct
       61 gtttgccaaa gagttccctg agctttgcgg caacccgttt gctctgttcc tcagtcatgg
      121 gaatcacggt tgtgactttg gcgtagagac agttacgcat ccggctgagc gttgccagga
      181 atttggcacg caggcgttcg tagtagatga agtcctcgtt tttaatgagt tgcctgagaa
      241 aggagagata ggcgtcactg aaatagatga tcagcatctc atcgacccac tccagttttt
      301 ctccgatgct cttcaagggg ttgaagagga tgtccgtgat ctcgaaattc tggctgcaga
      361 gaatatcgaa ggtaaaggcg tcccgcacaa aatcatcaag aagctcttcc tcctcgagca
      421 tcatgatcat cattggaagg tcgtagcggt aattctgcac ctgtcgtctc ctgcttccct
      481 tacacccgtg tcatcttttc aaactcgcgt atccggtttt cgagcacctg tgcctgttgc
      541 tcactggtca agcctgttcg caagacccgc tcacagccag agatgatcaa ctggttgatg
      601 cggtgaccgg tcagcgcaag gaactgttcg gattcggcgg ccagctccgc ttcacgctgc
      661 ttgcgcattt cgttcatcgc agcatgtgtt tccttctcga gttcctcaaa ccggctacgg
      721 gcccgttcgc gggcttcggc caggagttct tcacgttcgc ggtcgattgc agccagtctg
      781 cgggtaaggt ccgaagaagt tcgttcagcc ttgcgggcag cctcatcggc cgcctcaagg
      841 tcgctggaga ttttgttttt tcgctcatca atggccttgg tgatgggcgg gaggacaaat
      901 ttggacagga caatcagtac gataacaaag gagatgagag tccagatggc aacaccgggt
      961 tcaaacgtaa acacggaaga tcctccttga acgaaagcgt ctgcacatca cccgagggat
     1021 cagggctgtg gcgtttgatt gctgactgtg cttgtgacag caggtcgtgc accgtgagca
     1081 gctgcctgtg ctgtcggctg gggtgtcttg ctggccagca tgatcgctat gacgagtgca
     1141 tagaggacgg ccgcttcgat aaaggcaatc ccgacaaaga tcaatggcct gattcgactg
     1201 gtgatttcgg gctggcgggc gatgctttca aaggcacggc tcatcatgag tccgataccg
     1261 agggttccgc ccaatgcggc gatgctgatg gcgatggctg ctccgagtga gtccatgtga
     1321 aatgctcctt aatgtttttg ctccgcctcg gcgaccatca gggcgcttag aaaggcgaag
     1381 acgtaagcct ggatgaaact gacaaagagt tcaaagagga ggataacgat gcctccgaaa
     1441 acggaaaaga ccgggacaac cgcgtttgtt gcgcttgcgg cgaaagcgat aatcgtcagg
     1501 aagagggcgt gtccgccgga catattggca aagagacgaa cagccaggga aatcggtttg
     1561 aagagttggc tgacgagttc gatcggaaac atcagggcga cgaccggacc actgacgcca
     1621 gagggaatca ggctcttcca gaagccgaag ggcccatgaa ccttgaagcg caaggcaacg
     1681 gcgagggtga aaaacacgaa ggccagggca aatgtctcat tgaggttacc gctccccgag
     1741 gtggcacccg ggatgacgtt tgccagatcg ttgaaaaaaa tgaagaggaa gacggaaagg
     1801 aagaggggtg tccagcgccg ggtgtccagt tcatttggtt cgagaatctg ggaacgaaca
     1861 aactcgatgg tgatctcgaa gagattttgc gagagccggt ggggaatgcg agtcacccgg
     1921 cgccgggcca ggaaaccaaa cacaaggacc agtgcgaccg agatgaatgt ccagaggacg
     1981 gctgccgtga tcgagaggtc gattcctcca atgtggatcg ggacgagaac tgtcggtgtg
     2041 tgatgcatgg tgctgtaccc gcctgcctct tccgcgctgg cggtcgtgtc cgaaccggtc
     2101 aaatcgggtc cggaacgtgg ccgtcggtgg gacggggagt ccttcttcac atccggacgg
     2161 tcctgaccgt gccggtatag cgaagggtat gcaaatcgac ggaggaccgg caagaaaatt
     2221 cttgtgattc ggggttcctc gggcgggaat ggtccgcaat ccgtgttctc actgtcgaaa
     2281 agcgctgcaa acgtggttac atttaaggat ggaacgtgac gggcaagggg gcggatatgg
     2341 cagcaaaaga cagaccctca ttcaagcttg ggtacacctg tgttaccccg ggcgtggccg
     2401 ccctcctgcc tccagccctg atcatggaac tctttacccg gcacgaggcc cgcgactggg
     2461 gcgcgatcca gcccgaggac gccctcgaga atgaccggaa cgccgacagt ggcggccggg
     2521 tccggagcgt ctacctggtc tctcctgatc gcatccgtca ggctggcggg gttcccgcgc
     2581 cggcttccga aaatgatgcc atgccggatg acgcggtcga ggtctggatc atcaccgaac
     2641 cggaccggtt tgacagcatc ctcatgctcc ccggggagta cattgcctgg gagtccggtg
     2701 aaaagcgggt ctatcgcgaa cggctgcatg ccgctctcca ggccactccg gggcatgttt
     2761 cggccagtgc cgcccccggt gacgacaagt ctcaaaccgg acaacggccc ctgcccggcg
     2821 aagatttttt ttcgtggctt gaagagttct tcaccgccgt atactggaca cgctaccgtg
     2881 agcagcgctt cgagcggcgt tttgccaagg gcatgcttgt tcccggtctg gaccggatga
     2941 cggatgacgg actcgaaaag aacatcgacg aattccgcag ggaactggtc aaaacgatcc
     3001 gggacggtac cttccgcttc cgttttctcc ggcggattga tctggatgag gaagggaagc
     3061 gccggaccgt cgcccttccg gtgatgcggg accggcttct cgacaacatt ctcgcacgct
     3121 ttctttcgaa ccgtctcggg ctccggccac aggcccatgt ccttgtgacg agtcgccatg
     3181 tgcagaaact gatcacgggg gggcatgcct cctggttcat ccggacggac attgaaggat
     3241 tttttgacag cattgaccac accaagatca gggcatggct cgagaagcag ggtctgcagc
     3301 aggaggtcgg ccgtcttgtc tcggaactgg ccatcacccc gcggctgact ccggaagaga
     3361 tgacgctctt ccggacggag aatacccgga ccgggtggga gcaggccgtg cgtagtgcca
     3421 tgactccggt ccgggggctc ccgcagggtc tctatacctc caacatcctg gcagaggcct
     3481 ggcttgccga ctggattgca cgtcacccgc ttcccaagag gacggctctt gtgcggtacg
     3541 tcgatgattt tctgtttctc gccgaacgct ggtccggcct gcgggcactc aaacgctaca
     3601 tgaagcggat ggcaaaggat ctttctctcg ggttttccgc cgggaagacc gtttcgggcc
     3661 gggtcaggga tggcttcgat tttctcgggt tcttccaccg ccccgggtat gcccggagga
     3721 gtgaccgctc tgtccggcgt ttcaagatgc gctggatcga ggccctggag aacccgatgc
     3781 ggtttctgga gagccggatg gatccctggt acctgcacgg gttgatcgat accacggatg
     3841 acgccatgct ccgggagagt gtgatcttcg ggttggttgt gtactattac aatctctcaa
     3901 taattggctg gggccggacc tgggaaccgg ccaaccttct ggaatacgac aatctccagc
     3961 aggtgctccc gcagatcgtc gagggacagg tggagatcga ggagggcgag gccgacacgc
     4021 tccgcgagcg gtttgcggag cttggcaggg atcttttcga gatcaccatc tcggggagtg
     4081 cgctctacaa ttctttctgt acggatttca ggcagattga tgacctgaat cgctgggcag
     4141 gccggctctt ttcgggatat gcggcccgca agggtttgac aaaacccgtg cttgaagacc
     4201 atctcaagtg gtacttctat ttcagaaaac atcgcaccgg gtataaccgc aaggttctca
     4261 gggagtacat ccgtctttac
//
LOCUS       DAOSHE010000143         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18668_length_4274_cov_3.942166, whole genome shotgun sequence.
ACCESSION   DAOSHE010000143 DAOSHE010000000
VERSION     DAOSHE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18668_length_4274_cov_3.942166"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLD82_05135"
     CDS             complement(<1..581)
                     /locus_tag="PLD82_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG64802.1"
                     /translation="MEEIHENKAALVRLRGLEVSFSLGRASWFTAVDRIDLDIGQGEA
                     VGLVGESGCGKTVTALALMGLLPEVACRAGADLAEFDGAPFSIKPGYGPNGLARGRDV
                     SMVFQDALAALDPVFTIGAQLVEAIRVYAECTKEEARERAVTALAHVGIPDSESRMRA
                     YPHQLSGGMRQRVMIAMALLHRPRLLIADEPTTA"
     gene            complement(690..1088)
                     /locus_tag="PLD82_05140"
     CDS             complement(690..1088)
                     /locus_tag="PLD82_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG64803.1"
                     /translation="MILVDSNVLLDLFTNDPAWYDWSARALSQCAETDTLAVNPVIYS
                     EVSVGFERIEELDLCLPETLFKRLPLPWEAGFLAGKVFVRYRRAGGLRHSPLPDFFIG
                     AHAAVSGLRLITRDPKPYRTYFSTLELIAP"
     gene            complement(1085..1318)
                     /locus_tag="PLD82_05145"
     CDS             complement(1085..1318)
                     /locus_tag="PLD82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG64804.1"
                     /translation="MRITTKGQVTIPIDIRERMGLHPDSEVVFEVRENQVILRKSEQG
                     EGRLDAYLERVRGRASSRLSTDEIMALTRGSEP"
     gene            complement(1484..2248)
                     /locus_tag="PLD82_05150"
     CDS             complement(1484..2248)
                     /locus_tag="PLD82_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG64805.1"
                     /translation="MIEKIHALKKQLDALRPLLPEKVQALASVFQAEETEYIFESNAI
                     EGNTLTLAETELVLSKGITVSGKPLKDHLEATSHQKALARLKELVGERVPMDERTLLD
                     LHDLVLRGINDTYAGRYRDVPVRISGSRHIPPNPLKVPVLMEGLFAWFAGYAESAHPV
                     HAAADLHLRLSQIHPFVDGNGRTCRLMMNLHLLKHGYPLTIIRSENNRRVEYYNALAE
                     SDRTETPDAFRGFVEQRVLEALERYLAVMEEVAEFE"
     gene            complement(3221..>4274)
                     /gene="hcp"
                     /locus_tag="PLD82_05155"
                     /gene_synonym="priS"
     CDS             complement(3221..>4274)
                     /gene="hcp"
                     /locus_tag="PLD82_05155"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOG64806.1"
                     /translation="MKAGEVAVTTMALLDKANTETYGNPELTQVDIGVGNNPGILISG
                     HDLKDMEELLKQTEGTGVDVYTHGEMLPANYYPAFKKYSHFHGNYGSSWWLQDKEFES
                     FNGPILMTTNCLVPPKDSYKQRVFTTGNVAFPGVQHIADRPAGGVKDFSGLITLAKTC
                     QPPVELEKGTITGGFAHAQVMALADKVVEAVKSGAIRKFVVMGGCDGRQNGRNYYTEF
                     AEKLPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLDAGQCNDSYSLAVIALKLKEVFG
                     LADINELPIVYNIAWYEQKAVTVLLALLSLGVKNIHLGPTLPAFLSPAVVDVLVRTFG
                     ISPVGSVDDDLAKLVG"
BASE COUNT          899 a         1319 c         1194 g          862 t
ORIGIN      
        1 gcggttgtcg gttcatcggc gattaaaagc cgtggccggt gcaacaacgc catggcgatc
       61 atgacccgct ggcgcatgcc gcccgagagt tggtgaggat aggcccgcat ccggctttcg
      121 gaatcgggga tcccgacatg ggcaagagcc gtcaccgccc gttcccgggc ctcctctttt
      181 gtgcactccg catatacccg gatggcttcg accaattggg cgccaatggt gaagacgggg
      241 tccagtgccg cgagcgcatc ctgaaagacc atcgagacat cccgcccgcg ggccaggcca
      301 ttcggtccat aaccgggctt gatcgaaaac ggagccccgt cgaactcggc gagatccgca
      361 cctgcccggc aggcgacttc gggcaaaagc cccatcaggg caagcgccgt caccgtcttg
      421 ccgcaaccgg attcgccaac cagtccgacc gcctcgccct gcccgatatc caggtcaatc
      481 cggtcaaccg cggtaaacca cgacgcacgc ccgagggaaa acgaaacctc cagcccgcgc
      541 aatcgaacga gtgccgcctt gttctcgtga atctcttcca tacgtatcag tctatcatgg
      601 cgaacaatct caggcaagag agatgcggat tggcatgggg aggaagtctt tccccagagc
      661 aaggccgacc accggaggat ctgtcatttt tacggagcaa tcagttccag cgtggaaaaa
      721 tatgtacgat atggcttggg atcacgggtg atgagccgca gccccgaaac tgccgcatgg
      781 gcaccgatga aaaaatccgg caagggtgaa tgacgcaggc cccccgcccg ccggtagcgg
      841 acaaagacct tgcctgccaa aaagccggcc tcccagggaa ggggcaggcg cttgaacaag
      901 gtctcgggga ggcaaaggtc cagttcctcg atccgctcaa agccgacgga gacctcgctg
      961 tagatgaccg gattgaccgc cagggtatcc gtttcagcgc actgactgag tgccctggcc
     1021 gaccagtcat accaggcggg atcgttggta aagaggtcaa gcaggacatt gctgtccaca
     1081 agaatcatgg ctcgctgccc cgggtcaggg ccatgatctc gtcggtggag agccggctgg
     1141 aagcccgtcc ccgaaccctt tcgagatagg catccagacg cccctcaccc tgctcactct
     1201 tgcgcaggat gacctggttt tcgcggactt caaaaacaac ctcgctgtca ggatgcagcc
     1261 ccatccgctc acggatgtca atcggaatgg tcacctgccc cttggtcgtt attctcatgg
     1321 tacacctcct acaagtaaga gtaatacttt gcagggtatc tgtcaaggta tcatcacact
     1381 gaaacggcaa acgcaataat cagttgcgtg gcaagaatgg ccggatgata cacaaaagcc
     1441 agcgggtata caacgcagga aacgactttt cgagtttttc caattactcg aattccgcca
     1501 cctcttccat caccgccaga tacctttcca acgcctcgag cacccgctgc tcgacaaatc
     1561 cccggaaggc gtcgggcgtc tccgttcggt cactctcggc gagcgcgttg tagtactcga
     1621 cacgccggtt gttctcgctg cggatgatgg taagcggata cccgtgcttc aggagatgca
     1681 ggttcatcat gagccggcag gtgcggccat tgccgtccac aaacgggtgg atctggctca
     1741 agcgcagatg gagatcggca gcggcatgaa cggggtgtgc ggactccgcg tatccggcaa
     1801 accacgcgaa gagcccctcc atcagtacgg gcaccttcaa gggattgggc ggaatatgcc
     1861 gtgatccgga aatacgcacc ggaacatcgc ggtagcgtcc ggcataggtg tcattgatgc
     1921 cgcgcagcac aaggtcgtgc agatcaagga gggtacgttc gtccatcggc acccgctcgc
     1981 cgaccagttc cttgagccgc gccagcgcct tctggtggct ggtggcttcc aggtgatcct
     2041 tgaggggctt gccggagacg gtaatccctt tgctgaggac cagctcggtc tccgccaggg
     2101 tcagcgtgtt gccctcaatg gcgttcgact cgaagatata ctccgtctcc tcagcctgga
     2161 acaccgaggc cagagcctgc accttctccg gtaacagcgg acgcaaggcg tcgagctgtt
     2221 tcttcaacgc atgtatcttt tcaatcataa tcccagctcc ttcagatagc cggcaacttc
     2281 cacctcagac atcaatccgc aagcaaatta atttccgcga cagccgtata gggtttgccg
     2341 tcaatttccg acaacgcaac cagtttgaca tacctcgcag gagcggtgag ctcaagtgca
     2401 ctatctgttg ctgcttgatc cttcggaacg tcccgaacgc aacccgtgaa acagcttgcc
     2461 gtcctgactg gccagaatct cgtacctgcc gatatcccca ttgtagcagt actcgccatt
     2521 cgcataacgg ttttgacgcg gcagataccc gatacccctg acccaggtcg atcatgatcc
     2581 agtgcggatg catgggtgct ttgcccgtcc actgcgtatg ccaacaggta tccccgtctc
     2641 cgtcaatgac attcccgtcg acaccattct ccccgacacg ctctttgctg tcacaattga
     2701 tgactcgcca acccgccttg aattcgccca tcatcattgg acaggttttg ctataggcga
     2761 gatgaaccca cccccggtca tccccgtgct tcatgaaaaa gatctttttc aggtcccaat
     2821 cggaagcatc tctccaacgc cttgtccagt atacccgccg tgggcagtga ccgcaatatt
     2881 tttcccttgt gcatggcatc ccagcggccg gcatccgcgc cagggatcgg aagcggaaat
     2941 ggcggcccac aggtgcgcca ttcggagcgg gcaggtgcgg gtttgaaacc cgcatgtaca
     3001 atcgccagca ggccgggagg cgcgcccccc cccaaaaaaa agccggcccc ttgcggaacc
     3061 ggccggtcac tgcatctgtc catgttgtac gcaagaatct gtcccaaaag gcattgtgcg
     3121 aaaaagcatg attcgcaacc gggcacctcg ccagcacgcc aagggcctca gcgtgcggcg
     3181 gagcatgatt ttttgacctc ttgggacagt ccctgcagca tcacccgacc agtttggcca
     3241 gatcgtcgtc caccgaacca accgggctga tcccgaacgt cctgaccagc acatccacca
     3301 ccgccggaga gaggaaggcc ggcagggttg gccccaggtg gatgtttttg acaccgagcg
     3361 agaggagcgc gagaaggaca gtgacggcct tctgctcgta ccaggcaatg ttgtagacga
     3421 tcgggagctc gttgatgtcg gccaggccga agacttcctt gagcttgagg gcgatcacgg
     3481 ccagactgta gctgtcattg cactgcccgg cgtcgagcac gcgcggaatc ccgccgatgt
     3541 ccccgagggg cagcttgttg tagcggtact tggcgcagcc cgcggtcagg atgacggtgt
     3601 ccttgggcag cttctcggca aattcggtgt agtagtttct cccgttttgg cgcccgtcgc
     3661 agccacccat gaccacgaat ttgcggatgg cgcccgactt gacggcctca acgaccttgt
     3721 cagccagggc catcacctgg gcgtgggcaa atcctccggt gatcgttccc ttttcgagtt
     3781 cgaccggtgg ctgacaggtc ttggccagcg tgatcaggcc tgaaaagtcc ttgacgccgc
     3841 ccgcgggccg gtcagcgatg tgctgcactc ccgggaaggc cacgtttccg gtggtaaaga
     3901 cccgctgttt gtacgagtcc ttggggggga ccagacaatt ggtcgtcatc aaaatcgggc
     3961 cgttgaagga ttcgaactcc ttgtcctgca gccaccatga actcccgtaa ttcccgtgaa
     4021 aatgggaata ctttttgaaa gccggatagt aattggccgg aagcatctcg ccgtgggtgt
     4081 agacgtcgac gcccgtccct tccgtctgct tgaggagttc ttccatatcc ttgaggtcgt
     4141 gtcccgaaat caggatcccc ggattgtttc cgaccccgat atcgacctgg gtcagctcgg
     4201 gatttccgta ggtttcggta ttcgccttgt ccagcagggc catagttgtc accgccacct
     4261 caccggcctt catc
//
LOCUS       DAOSHE010000144         4255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18770_length_4255_cov_3.867381, whole genome shotgun sequence.
ACCESSION   DAOSHE010000144 DAOSHE010000000
VERSION     DAOSHE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18770_length_4255_cov_3.867381"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLD82_05160"
     CDS             complement(<1..344)
                     /locus_tag="PLD82_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG64807.1"
                     /translation="MNRHVYLDNNATTPLDPDVLAAFSDALARFGNPSSMHGFGREAA
                     NLLSDARAEAAAILGAEPGEITFTSGGTESNNTVLASFAPGIPVTDGVTPPPSGKNEI
                     IVSAIEHPAILET"
     gene            complement(341..784)
                     /locus_tag="PLD82_05165"
     CDS             complement(341..784)
                     /locus_tag="PLD82_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG64808.1"
                     /translation="MFSVPTRIRYALRALIRLRSHNPASGPMALHQIAKDESISIKYL
                     ESIFTQLRRAGIVQSVRGADGGYHLAQPASEISLLRILEAVEGPLVTVDCLQHPERCP
                     QKDVCRTYSLWDELQIQLETFFREKTLETLSTCQVGEISTKGRRQ"
     gene            complement(1192..2475)
                     /locus_tag="PLD82_05170"
     CDS             complement(1192..2475)
                     /locus_tag="PLD82_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64809.1"
                     /translation="MRKTFFILFAFSLLFVFAACGDDETSSTTPTTTTGTLPSRYAVE
                     IPKTLKGGSTTPTAPRRVKGLAPESMALQQIKSDVAQFEMMQKQVPMQFVLVDAVMPQ
                     LLTLIGDKTEVTATNLTMTITQSVLDKIKDLMGEELYNTEFAPMASTMLNQPFPIDPI
                     TYRKLSGDPLYDHLIVIASTNEISGDSMTIRWNNAKTKVSILNTFAFSGVETSQLCTY
                     DDTKKTSFMSFKDNMGMSMVMALAQTNMTKNGVLIKSTTKMTNGGEYTYESVGMADDD
                     GGYVEMFSTSGTDKWGYRESFDGLGNQTGYAFKGPTDSDWVVQNQFDDSTYYTEDTSS
                     YAVAEVKVTVSGLSAECDFFLVPPGTTTIDETTTMLGSGHYDGASMTNDTSWVMYWGD
                     ESAASTALVFKSEFNGTDMTFSQIVGAAVSVVPAP"
     gene            2674..3537
                     /gene="nadC"
                     /locus_tag="PLD82_05175"
     CDS             2674..3537
                     /gene="nadC"
                     /locus_tag="PLD82_05175"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG64810.1"
                     /translation="MMRSLVEYMSLDEISRMVEQALVEDVGPGDVTTEACIPFDAMDA
                     ALIRAKGAGVIAGLDFAREVFVQLDRQAVFEPVVEDGTRVVPGDPVCRISGRARALLT
                     GERTALNFLGHLSGIATAAARVSALTEGTPLTVLDTRKTTPLLRQAEKYAVQAGGCRN
                     HRTGLYDMVLIKENHIRAAGGITQAVRRARSVAPGLKIEVETTCEAEVREALAAGADR
                     IMLDNMDDAMVCSMLSIINGQAETEASGNMTLERITALASSGLDYISMGSLTHSVTVL
                     DLSMLFERGGC"
     gene            complement(3679..>4255)
                     /locus_tag="PLD82_05180"
     CDS             complement(3679..>4255)
                     /locus_tag="PLD82_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG64811.1"
                     /translation="HLGAGMIETSLALHISLYNIVLFSLGLFILEHSKGSHGGSLRIL
                     RSVGTNPLLLSVVAGVALAVFDIPLPKAVEDVIALLSNSATAVVLFGLGLFLPDVRFE
                     RKTLGHVVALTTISLVAVPALFLAAYALFQPGREFLVSILEGAMPLAVSPFVLGASYP
                     LDRPVIAGSILVSTALSVVTIPIMLGLAQGL"
BASE COUNT          882 a         1076 c         1353 g          944 t
ORIGIN      
        1 gtctcaagga tggccggatg ctcgatggcg ctgacaatga tctcgttttt tccggagggc
       61 ggtggagtga cgccgtctgt tacgggaata cccggggcga aggatgccag aacagtattg
      121 ttggattccg ttccgcccga ggtaaaggta atttcacccg gttcggcgcc gaggatggcc
      181 gccgcttcgg ctcgggcatc ggataacagg ttggccgctt cccggccaaa gccatgcatg
      241 ctggatggat tgccgaatct ggccagggca tccgagaatg ccgcgagtac atcggggtcg
      301 agaggggttg ttgcgttgtt gtcgaggtag acgtgtctgt tcactggcgg cgtcctttcg
      361 tggagatctc gccaacctgg caggtcgaga gggtctcaag tgttttctct ctgaaaaagg
      421 tttcaagttg gatctgcagt tcatcccaga gggaataggt tcggcagaca tctttttgcg
      481 ggcagcgctc ggggtgttgc aggcagtcaa cggtgacgag agggccttcg acggcttcga
      541 ggatcctgag gagggagatc tccgaggccg gctgtgcaag atggtatcca ccgtccgctc
      601 cgcggacact ctggacgatt ccggcccggc ggagctgggt aaaaatgctc tcgagatatt
      661 tgatggagat gctttcgtct ttcgcaatct ggtgcagggc catcggtcca gatgccgggt
      721 tgtggcttcg caggcggatc agggcccgca gggcgtaacg aatccgtgtc ggtacactga
      781 acatcttgcc ctccaatccc tacagtcata taggaattat atactcaccg tgtgtgtcag
      841 gcaaggaaaa agacagtaat ggacgaagta cccacgatga ttttgggttc ggcaaagtca
      901 gtatagagtg aatatgcaat ttgcaatacc gtttcttgtg agaggcaaga gttttcaggg
      961 cagcaggttc gactgtcatc atcatgctgg cttgagcgtg ctcatgcttg tcctgaaggc
     1021 ggaaaaaacc gccccttgcg ggacggtttt tgatgatcgg cgggatcccg atccgcatca
     1081 gttggccttg gttacagcag ccgctgaatc gattccggga ttgtcccatt tgacaaaaaa
     1141 ggtgcgcccc ttgcggagcg cacccggttg atcgaacaag agacaggaag attatggcgc
     1201 tggaacaacg ctgactgccg cacccacgat ctggctgaag gtcatgtctg taccattgaa
     1261 ctctgacttg aagacgagag ctgttgatgc agcggattca tcaccccagt acatcaccca
     1321 ggatgtgtcg ttggtcatgc tggcgccatc ataatgaccg gagccaagca tggtggtggt
     1381 ttcgtcgatt gttgttgtgc cagggggaac aaggaagaaa tcacattcag ccgacaggcc
     1441 gctgacggtg actttgactt cggccaccgc atacgagctg gtgtcttcgg tatagtaggt
     1501 tgaatcgtcg aattgattct ggacgaccca gtcggagtct gtcggccctt tgaatgcgta
     1561 cccggtttgg ttgcccagtc cgtcaaagct ctcgcgatag ccccacttgt ccgtgccgga
     1621 ggttgagaac atctcgacgt agccgccgtc atcatccgcc ataccgacgc tttcataggt
     1681 atattcacca ccgttggtca tcttggtggt gctcttgatc agaaccccgt ttttggtcat
     1741 attcgtctgt gccaaggcca tgaccatcga catgcccata ttgtccttga aactcatgaa
     1801 actggttttt ttggtatcgt cgtaggtgca gagctgggag gtttcgactc cgctgaaggc
     1861 gaaagtattg aggatgctga ccttggtttt cgcgttgttc cagcggatgg tcatgctgtc
     1921 accactgatt tcgttggtgc ttgcgatgac gatcaggtga tcatagagtg ggtcgccgga
     1981 aagttttctg taggtgatcg gatcgatcgg gaaaggttgg ttcagcatgg tgctagccat
     2041 cggggcgaac tcggtgttgt agagctcttc tcccatcaga tccttgatct tgtcaagcac
     2101 gctttgtgtg atggtcatgg tcagattggt tgctgtcacc tcggtcttgt cgccaatcag
     2161 cgtgagcagt tggggcatga cggcatccac aaggacaaac tgcatcggga cctgcttttg
     2221 catcatctcg aactgggcga catcgctctt gatctgctgc agtgccatcg actcgggagc
     2281 caatcccttg acgcgccggg gtgctgtcgg tgttgtcgaa ccacccttga gggtcttggg
     2341 aatttcgaca gcatagcgtg aggggagtgt gcctgtcgtc gtcgttggcg tcgtgctgct
     2401 tgtttcgtcg tcaccgcagg cggcaaagac aaagagcagg gagaaggcaa aaagaataaa
     2461 gaacgttttt ctcatacaga gtgttcctcc gaatctggat tggcccggct tctccgggcc
     2521 gattcggata gtatggtata cccgaagctg gagtttatca ataaagttcc agtgatatcc
     2581 tgaaattcaa ggaatgcccg atatttgtcc cggagaaaag cccttggcta tacttgaacc
     2641 cgggtgaacc cgtaatcgat ggcggagggt ggcatgatgc ggagtcttgt tgaatacatg
     2701 tctttggacg agatcagtcg gatggttgag caggcattgg tcgaggatgt cgggccgggt
     2761 gacgtgacga cggaggcgtg cattccgttc gatgccatgg atgccgcact gattcgcgcc
     2821 aagggtgcag gcgtgatcgc cggtctggac tttgccaggg aggtttttgt tcagctcgac
     2881 cggcaggctg tcttcgaacc cgttgtcgaa gacgggacgc gggttgttcc gggagatccg
     2941 gtctgccgga tctccgggcg tgccagggcc ctcctgaccg gtgagcggac ggcactcaat
     3001 tttcttggac atctctcggg catcgccacg gccgcggccc gtgtatccgc gctgactgag
     3061 gggacaccct tgacggttct cgacacccgc aaaacgacgc cgcttctgcg gcaggctgaa
     3121 aaatacgccg ttcaggccgg tggctgccgc aatcaccgta ccggactgta tgacatggtg
     3181 ttgatcaagg aaaaccacat ccgggccgcg ggtggaatca cccaggccgt ccggcgtgca
     3241 cgaagtgtgg cacccggact caagatcgaa gtcgagacga cctgcgaagc cgaggtgcgg
     3301 gaggcactcg ctgccggtgc ggaccggatc atgctggaca atatggacga cgccatggta
     3361 tgcagcatgc tttccataat aaacgggcag gcagagacgg aagcatcggg caatatgacg
     3421 ctcgagcgga ttacggcgct ggcctcaagc gggctggact acatctccat gggctctctg
     3481 acccactcgg tgacggtcct tgatctctcg atgctgtttg agcggggggg ctgctgatgt
     3541 cggggcataa acagttcggg cgagccggct cctcgcgggt ctgactgtgt tcatcatact
     3601 ctgtagcccc gcacaggagg cccataccca aaccgagggg tacccgtcca taccaggtcg
     3661 gaccggttcc accggccatc agagtccttg ggccagaccg agcatgatcg ggatggtgac
     3721 aacggagagg gctgtcgaga ccaggatcga gccggcgatg acgggccggt ccaagggata
     3781 gctggcacca aggacaaagg gcgagacggc caggggcatg gcgccttcga ggatcgagac
     3841 gaggaattcc ctgccgggct ggaaaagggc gtacgctgcg agaaagaggg cggggacggc
     3901 gaccaggctg atggtggtca gggcgacgac gtgtcccagt gttttgcgct cgaagcggac
     3961 atccggcaaa aagaggccga gcccgaagag gaccacggcg gtggcactgt tgctcaggag
     4021 ggcgatcaca tcctcgactg ccttcgggag aggaatgtcg aagacagcca gggcaacacc
     4081 tgcgaccacc gagaggagga gcgggtttgt gcccaccgag cgcaggatgc gcagacttcc
     4141 gccgtgactg cctttggaat gctcgagaat gaagagtccg agcgagaaga gaacgatgtt
     4201 atagagcgag atgtgcaggg cgagggatgt ttcgatcatg ccggcaccca gatgc
//
LOCUS       DAOSHE010000145         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18822_length_4246_cov_3.632785, whole genome shotgun sequence.
ACCESSION   DAOSHE010000145 DAOSHE010000000
VERSION     DAOSHE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18822_length_4246_cov_3.632785"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1470)
                     /locus_tag="PLD82_05185"
     CDS             complement(4..1470)
                     /locus_tag="PLD82_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64812.1"
                     /translation="MKAWMQGVVAVVLFFVLSNGMVGAEQDVKPRRIYVTMDASGSML
                     NQLDARIIRAIRQTVSQILFEGRYEAAAGIRIVTDNSGILKDRPLFRGGDDRWSFWSY
                     GTTLDRKIDESTSDQTGALLDLLPQDRDGFAAWFKQPDTLLYLPEMALLEKFDSEHEV
                     FWIDISDNVFDGGVNAIPQALLEKWDRLHEGVNTQFVLFRARAANGIFVTMRKVADDS
                     SFKLRFMTGDEQRVVLVYDKTAESLWNLESLPAQIVADKGSLPTMRIRGIELELRDPE
                     SKKTFRVKARIKSDDLSSLKDVVFEDKTDTAGMELVKRQKQAFVRVIYQDVHNREYRY
                     VFRNLPLLLQEKNATSFFVTLLLILAVLAGLALLFALTRLILGQFKPRWPVELSLALA
                     GSFGKEEKFVLNEDVHKIVFDPELRGESTDCHLFRGIKMEQARLDITPDRKGIRCLVT
                     PGDGSPAMPRFIEDSGEVMLKNKFGKEVAIRIEILKQD"
     gene            complement(1485..3002)
                     /locus_tag="PLD82_05190"
     CDS             complement(1485..3002)
                     /locus_tag="PLD82_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64813.1"
                     /translation="MKCPYCNHADDAANSLDPDEYTGCICPVCRRVLLLCPHCRQLNQ
                     GSAVYCRQCSQRLRSRQHIGGVSSINNYFPSEENISTGRIDLPIVHKWSDRFQYDDSE
                     LVDMLFFRDVLVLRNKIAGKIEIRQSFRPENAPWLYDYAPAPDEECQLAVIEDLVVFA
                     ERDKTGKLLVKTVDVSALGIAEHPSPAVTLYEVDFGCEGFAVFPFGTADVKDQILCLI
                     TPHKYYFWMYSKIIQSRMNGSERVEPSLVVTTEGLESDEVFRAPLVHDRASSLFCTTS
                     KGRVFSLPLSSIRSVLTGMGQHDLVATCSGGLPAGWLASAPAPLDENFAIEAVRREDG
                     ARVLATWSPEYDEFRFFDAICTEFEGEFHSLAEKPVLLASALRRPPPYMRNEHAECVV
                     MIGCFELDTRTEETRRYHVVNSSGFRQRFGRLSLSRGRTLEGSEIWTDGSRIGIFSGA
                     SSMDIGDILRDGELNVTHSVIPEFVPSDGKSVVSPVRRYARFGASAGLDMVVIHF"
     gene            complement(3532..>4246)
                     /locus_tag="PLD82_05195"
     CDS             complement(3532..>4246)
                     /locus_tag="PLD82_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64814.1"
                     /translation="ACIVLLAKNKAIRSVWRIDTKEEGRMEIPLTLGVYALTDLVPGS
                     GYEFAVHMEDGSWQRLCCTVVEDVPGMAGREWARALTRALAAGRVRAFDAMIGSSDPA
                     DAAVKGLQYMRTLLSGLFSKLWLTIQQQINQRGPGSDRGSTPEAVGQAELATETGIAA
                     YAGARAGAWLLWKSGDTDGARSLMEKALRLADPSVVSPETIRQMETELHTMQSPETAP
                     DLDAPFAVFDALWPDGTVE"
BASE COUNT         1096 a         1197 c         1020 g          933 t
ORIGIN      
        1 agttcagtct tgtttcagaa tttctatgcg gatggcaact tccttgccaa acttgttttt
       61 aagcatgacc tcgccactgt cctcgatgaa tcgaggcatg gcaggtgagc catcgccggg
      121 tgtcacgaga cagcggatcc cttttcgatc aggggtgata tcaagcctgg cctgttccat
      181 cttgatcccg cggaagagat ggcaatccgt actctcaccg cgcaactcgg gatcaaagac
      241 aatcttgtgg acatcctcat tgaggacaaa tttttcttct ttgccaaaac tgcccgccag
      301 agccagagac agttcaacag gccaacgggg cttgaactgt ccgagaatca atcgtgtgag
      361 agcaaacagc agcgccaacc cggccaggac agcaagaatg agcaggagcg tcacaaaaaa
      421 tgaggtcgcg tttttttcct gcaacaggag aggaagattg cggaagacat agcggtattc
      481 ccggttatgg acatcctgat agatgacccg cacaaatgcc tgtttctgcc tcttgaccag
      541 ttccattccg gccgtatccg tcttgtcctc aaagacaaca tctttgaggg atgaaagatc
      601 atcgctcttg atcctggcct tgacgcggaa ggtttttttg ctctccggat cacgcaactc
      661 caattcgatc ccccggatgc gcatcgttgg cagggagcct ttatcggcaa cgatctgtgc
      721 cggaagactc tccagattcc acaaagattc agccgtcttg tcgtagacaa gaaccaccct
      781 ctgctcatcc cccgtcatga aacgaagttt gaacgaagaa tcatctgcga cttttcgcat
      841 tgtcacaaaa atgccattgg ccgcccgggc ccggaagagg acaaactggg tgttgacacc
      901 ctcatgcagc cggtcccatt tttcgagcag cgcctgagga atagcattca ccccgccgtc
      961 aaacacgttg tcactgatgt cgatccagaa cacctcgtgc tcactgtcaa atttttcaag
     1021 aagggccatc tcgggcaaat agagcaaggt gtctggctgc ttgaaccagg ctgcaaagcc
     1081 atcccggtcc tgtggcagaa gatcaaggag cgcaccggtc tggtccgagg tactctcatc
     1141 gattttccgg tcaagggttg tcccataact ccagaatgac cagcgatcat ccccccccct
     1201 gaagaggggc ctgtctttga gaataccgga attgtccgtg acaatcctga tacctgccgc
     1261 tgcctcgtag cggccctcaa aaagaatctg tgacacggtc tgacggatcg cacggatgat
     1321 ccttgcatca agctggttaa gcatgctccc cgaggcatcc atggtcacat agatcctccg
     1381 cggctttaca tcctgctctg ccccaaccat accatttgaa agcacaaaaa aaagtacaac
     1441 ggcgactaca ccctgcatcc aggccttcat atttcctccg acattcaaaa atggatgaca
     1501 accatatcaa gaccggccga tgcaccaaag cgggcgtagc gacggaccgg cgaaacaacc
     1561 gactttccat ccgaaggtac aaattcagga atcactgaat gcgtgacatt cagttccccg
     1621 tctctcagga tatcaccaat atccatcgaa gatgccccgg aaaagatccc gatccgggat
     1681 ccatccgtcc agatctcgct gccttcaagg gttcgccctc gggaaaggct aagacgacca
     1741 aagcgttgcc tgaaaccact gctgttgaca acatggtacc tgcgcgtttc ttcggtcctt
     1801 gtatccaact caaaacaccc gatcatgaca acgcactcag catgttcatt gcgcatatag
     1861 ggcggcgggc gtctcagagc agatgccagc aaaaccggtt tttcagcgag agagtgaaat
     1921 tcgccttcaa attcggtaca gatggcatca aaaaaccgga actcgtcgta ctcgggagac
     1981 catgtcgcaa gaacacgggc accatcttct ctgcgaacag cttctattgc aaaattctca
     2041 tccaggggcg ccggtgccga tgccaaccaa ccagcgggca atccacccga acacgtggca
     2101 acaagatcat gttgtcccat gccagtcaac accgagcgga tactgctgag gggcagtgag
     2161 aagacccgtc ccttggatgt cgtgcaaaat aaactggagg cccggtcatg gaccaacggt
     2221 gcccggaaca cctcgtcaga ttcaagtcct tcggtcgtaa ccacaagaga cggttcaacc
     2281 ctctcggatc cattcattcg tgattgtata atttttgaat acatccagaa ataatatttg
     2341 tgtggcgtga tcaggcagag gatctgatcc ttgacgtccg ctgtcccgaa tggaaagact
     2401 gcaaatcctt cacacccgaa atcgacttca tacagagtga ctgctggaga tggatgctcg
     2461 gcgatcccaa gtgccgagac atcgacggtc ttgacaagca attttccagt tttgtctctc
     2521 tcggcaaaca caacaagatc ttcgatgacg gcaagctgac actcctcatc gggagcagga
     2581 gcataatcat agagccatgg ggcattttcg gggcggaagg actgtctgat ctcgatcttc
     2641 ccggctattt tattgcgcaa aacaagcaca tcccggaaaa acagcatatc gaccaactcg
     2701 ctatcatcgt actgaaagcg gtcactccat ttatggacga tgggcaaatc aatccggcca
     2761 gtggagatat tctcttccga agggaaatag ttattaatag aagaaacgcc acctatatgc
     2821 tgccgacttc tcaggcgctg gctgcattga cggcaataga ctgccgaacc ttggttgagc
     2881 tgcctgcaat gcgggcaaag cagcaagacg cgccgacaga cgggacagat acatccggtg
     2941 tactcatcag gatcgagtga atttgccgca tcatcggcat ggttgcagta gggacatttc
     3001 attaatacac tctccatggc gcaggcagta taaaccagtc acctgtattt ttcaagagcc
     3061 gtctttgaat ttatccaaat cacttgcgct acacccagga tccggataca ttccataata
     3121 cgtaccagtc acgcatactg ttgcatgagg ctgtctcacc acatatcgga caggtgtatc
     3181 ttgccaaacc gtccggtgaa ttgaatttac ttgaaagtcc agtgatattc agaaaaatga
     3241 tagcaaaccg ctcagacgtt cttgaaatca aaacagcggg gtattcgaga gcactccgac
     3301 aagatgtgtc tcgggcaact acaatctacc tgcaaaacgg gctccagggt aattacaatc
     3361 ccaacctgca gaccatttgg gtaaacacga ttccccagcc agggtactca gaatgatccc
     3421 aagtccgcaa cctgtacaaa aggctggctg acgaaaaacc aatcggattc ttctctatcg
     3481 atgcgctcaa ccacaatgca tggcttgcgc atcaaacaga cacggtcagt cttattcaac
     3541 tgtcccatcg ggccagaggg catcgaacac ggcaaagggc gcatccagat ccggggcagt
     3601 ctccggtgat tgcatggtgt gtaattccgt ctccatctgt ctgatcgttt caggggagac
     3661 aaccgatgga tcggcaaggc gcaaggcttt ttccattaac gagcgggcac cgtcagtatc
     3721 accggatttc cagagaagcc aggcgccagc cctcgcgcca gcatacgcgg caatacctgt
     3781 ttctgttgcc aattccgctt ggcccacggc ttccggcgtt gacccgcgat cgcttcccgg
     3841 accacgctga ttgatctgtt gttggattgt cagccagagt tttgaaaaaa gcccggaaag
     3901 aagcgtccgc atgtattgca agcccttgac ggccgcatct gccggatcgg aagagccgat
     3961 catcgcatcg aatgcgcgaa cacgaccggc ggcaagcgca cgtgtcagcg cccgtgccca
     4021 ttcccgcccg gccattccgg gaacatcctc aaccacggta cagcagagtc tttgccagga
     4081 cccgtcttcc atgtggacag caaattcata tcctgaacct ggcacaagat ccgtcagagc
     4141 gtacacacca agggtcaggg gaatctccat ccgaccctct tcctttgtat cgatacgcca
     4201 gactgaccgg atggccttgt ttttggcaag cagcacaatg caggca
//
LOCUS       DAOSHE010000146         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_18847_length_4243_cov_4.027459, whole genome shotgun sequence.
ACCESSION   DAOSHE010000146 DAOSHE010000000
VERSION     DAOSHE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18847_length_4243_cov_4.027459"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLD82_05200"
     CDS             complement(<1..619)
                     /locus_tag="PLD82_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64815.1"
                     /translation="MKRILIPLACLFVAVASVPALAQEGSTNTRSKPDGLVRSTAWSV
                     YFNNYLYRFSNGDLRKTDNTSIQTVENTDDEFFIGYLELGAIFEANLAHGINFYVDLY
                     RVGFWGNDSPEYVAANPVYFRTLNFSLGLFEGASLVVGRQRYSVNPDRAHHNYVLTDN
                     IDAVLFSLDQKSWGVDVFADLFSMNSPVDAVYELHADRHSGTVDYF"
     gene            complement(658..2376)
                     /locus_tag="PLD82_05205"
     CDS             complement(658..2376)
                     /locus_tag="PLD82_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOG64816.1"
                     /translation="MKLASSMRLVTAAACALLAACSAADISEPALSPWPRGLQVWFSE
                     PGISPSTGHDQQLPEKVGKLILSAKKSVDINIYELSVPAIYQALIEVHKKGIKVRMVG
                     DIDNTFYEGYKALMAARVPMRLGNPEKIMHNKFVIVDDHIVTMGSMNYTGSGALLNNE
                     NVVFITSSNVAAYYKKEMDNMFVNGTFGLEKKPFDGFADNRFVVNPGQPDETKIEVYF
                     TPYVGAFAPNYNANTRILEIISNARHTIHFAMFAFTSTEIANAIIWKATNQGVRVYGV
                     FDKGWHESNEYSVHQRMMDAGLNIVMDGNENFDPHNPYHGSKVHDKIMVIDAGYDSAL
                     TLTGSFNFSPSAAVDGNDENCIIISNRSISLRYRDELVRLYTQGRHPSRSLGGDKAGW
                     LDVIINEINWAGSQSDSGTKNYNDKFIELRNRTGRAINISGWQLWGTCSKSYRIYGHI
                     FPANTVIAAGGYHVLGYSPYSSAFAWDSTWNDYMLLKDFHNASDQNYVMLTLKDAWQN
                     HVDRAGHPSQVPYAGLQPSSGAFASMQRTALDGTNAASWATTTTAGAGVRTGWKTRTL
                     ATPGSD"
     gene            complement(2391..3041)
                     /locus_tag="PLD82_05210"
     CDS             complement(2391..3041)
                     /locus_tag="PLD82_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG64817.1"
                     /translation="MKLKFARYAVFSLVLAGCAIGEPSETRYNVSPYDIRGEIYVSEV
                     HWAGSVDNTGAKNNPDDDFIEIYNNSSAPYDISGWCLVFSGTTSQLIVFPTNTVIPSG
                     RYYTVGNSTNGAFNNLDLVIPGFSIPSSRFMIQVQDAGGRTADSVDFMQKDYLPAGAA
                     LPRIRRSAIRKTDFFGGEEGSSYDSWGEYLGHETAWAMQYIRGNYQGAVFCSPGNR"
     gene            complement(3054..>4243)
                     /locus_tag="PLD82_05215"
     CDS             complement(3054..>4243)
                     /locus_tag="PLD82_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HOG64818.1"
                     /translation="SGELPDQYERKFLPDPFLSGLSTSLSTALVPAFGIERTNYMIGV
                     PAGTASFTITPVSRSSTSRITVNGETVLSGMASQPIVPATGTTRVPVRVTDRDGLSVF
                     TYTVSAVLLTGSENGDSSLSSLVVGPTNILSFTPATTTYSCAVNFDITSASVTPAASH
                     AAARIFVNNQQVASGSSLAIPLAVGVNTVSIRVVAENFTETTYTVTITRTTPALFGLL
                     ISEWADAGTNVDYIELWNSGSQSLSITRDFRILIGTTEISLSKYGTSSNVSVFASIPS
                     LGGVTIAPDEVILIVDSDVNATNLNTIINWGVPAGTKFFTSSESTLVGTGDRLNQNRA
                     FLKSGSYEWSHTPDPAAFTAALMTSTYMKLRATFNPATDAYTNIAVWQNGSTAIRSPG
                     TRN"
BASE COUNT          938 a         1072 c         1229 g         1004 t
ORIGIN      
        1 caaaataatc gactgttccc gaatggcggt cagcatgcag ttcatagacc gcatcaaccg
       61 gagaattcat ggaaaagaga tcggcaaaaa cgtctactcc ccatgacttt tggtcaagcg
      121 agaagaggac ggcgtcgatg ttgtcggtca ggacatagtt gtgatgtgcg cggtccggat
      181 tgaccgaata gcgctggcgt ccgacaacaa gactggcgcc ctcaaagaga ccgagagaaa
      241 aattgagcgt cctgaagtag acaggatttg ccgcaacata ctcgggcgaa tcgtttcccc
      301 aaaaccctac cctgtagaga tcaacataaa aattgatccc gtgggcaagg ttggcttcaa
      361 aaattgcgcc cagctcaaga tacccgataa aaaactcgtc atccgtgttt tcaaccgtct
      421 gaatgctggt attgtcagtc ttgcgcaggt caccattgct gaagcgatac agataattgt
      481 tgaagtagac agaccaggct gtagagcgca caagtccatc aggcttgctt ctggtgttgg
      541 tgcttccttc ctgggccaga gccggaactg aagcaacagc tacaaacagg caggcgagcg
      601 gaatcaggat acgtttcatg atttctcctt gttgagtatc aggctggcag cctgttgtca
      661 gtcacttccc ggggtggcca gggtccttgt cttccagccg gtgcgtaccc ctgcaccagc
      721 cgtggtcgtc gtcgcccagc ttgcggcgtt tgttccgtca agggctgttc gctgcatcga
      781 agcaaatgct ccgcttgagg gctgaagacc ggcatacggt acctgactcg ggtgcccggc
      841 acgatcgaca tggttttgcc aggcgtcctt gagggtcagc atgacgtagt tttggtcact
      901 ggcgttgtga aaatccttga ggagcatgta gtcattccag gtactgtccc aggcaaaggc
      961 gcttgagtat ggagagtatc cgaggacatg gtaccctccc gcagcaatga cagtgtttgc
     1021 ggggaagata tgcccataga tccggtagct cttgctgcag gtcccccaga gttgccaacc
     1081 cgaaatattg atcgcccgtc ctgtccggtt gcgcaattca atgaacttgt cgttgtagtt
     1141 ttttgttccg ctgtcggact ggctgcccgc ccagttgatc tcattgatga tgacatccag
     1201 ccagcctgcc ttgtccccgc cgaggctgcg ggacggatgc ctgccctgtg tgtacaaccg
     1261 gacgagctcg tcacggtagc ggagggagat ggaccggttt gagatgatga tgcagttttc
     1321 gtcgtttccg tcaacggcgg cagatgggga aaagttgaac gaaccggtca gggtcagggc
     1381 tgaatcgtac cccgcatcaa tgaccatgat cttgtcatgc acctttgagc cgtgataggg
     1441 attgtgcggg tcgaaatttt cgtttccgtc catgacaata ttgaggccgg catccatcat
     1501 ccgctgatgg acagaatact cattggactc gtgccagccc ttgtcgaata caccgtagac
     1561 acgcacgccc tggttggtcg ccttccagat gatggcgttg gcgatttcag tgctggtaaa
     1621 ggcaaacatg gcaaagtgga tggtatgccg ggcattgctg ataatctcga gaattcgtgt
     1681 attggcgttg tagttggggg caaaggcccc gacatagggc gtaaagtaga cttcgatttt
     1741 tgtttcatcc ggctgtccgg gattgacaac aaaccggtta tcggcaaacc catcaaaggg
     1801 ctttttttcg agaccgaagg tcccattgac aaacatgtta tccatctctt ttttgtaata
     1861 cgccgccaca ttgctgctgg taataaaaac cacattttcg ttgttgagga gagctcccga
     1921 gccggtatag ttcatcgaac ccatggtcac gatgtggtcg tcgacgatca cgaatttgtt
     1981 gtgcatgatc ttttcggggt tgccaagcct catcgggacc cgtgcggcca tgagagcctt
     2041 gtatccctcg taaaatgtgt tgtcgatatc tcctaccatg cgcaccttga tgcccttttt
     2101 atgcacttcg atcaaagcct ggtaaattgc cggaaccgag agctcgtaga tattgatgtc
     2161 cacacttttt ttggcgctca ggatcagctt gcccaccttt tcgggcagtt gctggtcatg
     2221 cccggttgag ggtgaaatgc ccggctccga aaaccagacc tgcaggcccc tgggccaggg
     2281 agagagggct ggttcggaaa tatcggccgc cgagcaggcc gcgagcagtg cgcaggccgc
     2341 cgcagtcaca aggcgcattg aagatgccag tttcatggtc ctcccctttt ttaccggtta
     2401 ccgggtgaac agaagacggc accctgatag tttccgcgga tgtactgcat ggcccaggca
     2461 gtttcatggc ccagatactc gccccagctg tcgtaactcg acccttcttc accgccaaaa
     2521 aaatcggtct tgcggatggc actgcgcctg atcctgggga gcgcggctcc ggccgggagg
     2581 tagtcctttt gcatgaaatc aacgctgtct gcagtccggc cacccgcgtc ctggacctga
     2641 atcatgaagc gtgacgaagg gatggaaaaa cccgggatga caagatcaag gttgttgaag
     2701 gccccgtttg tgctgttgcc gactgtgtaa tagcggcctg acggaatgac ggtgttggtc
     2761 ggaaagacga tcagctgcga tgttgttccg gaaaacacaa ggcaccagcc cgagatgtcg
     2821 tagggggcac tcgaattatt gtaaatctcg ataaaatcgt catccgggtt gttttttgcc
     2881 ccggtattgt caacgcttcc ggcccagtgt acttcggaga cgtagatctc gccacgaata
     2941 tcgtagggcg atacgttgta gcgggtctca gacggctcgc cgatcgcaca ccctgcgagc
     3001 acaagggaga acactgcata gcgggcgaat ttcagtttca tcaatcactc tccctaattc
     3061 cgggttccgg gactgcggat ggctgtcgag ccgttttgcc agactgcgat attggtatag
     3121 gcgtccgtcg cagggttgaa ggttgccctg agtttcatat atgttgaggt catcaatgcc
     3181 gcggtaaagg ccgccgggtc aggggtgtgg ctccactcgt acgagccgga cttgagaaag
     3241 gccctgtttt ggttgagtct gtcacctgtt ccgaccaggg ttgactcgga agaggtaaag
     3301 aactttgttc cggcaggcac tccccaattg atgatggtat tgagattggt ggcgttgaca
     3361 tccgaatcca caatcaggat cacctcgtcg ggggcaatcg tcaccccgcc caaacttggt
     3421 atcgaggcaa aaacgctcac gttgctgctt gtaccatatt ttgacagact gatttcggtt
     3481 gtcccgatca ggattctgaa gtcccgggtg atggacagac tctgtgagcc ggaattccag
     3541 agctcgatat aatcgacgtt tgtcccggca tcagcccact cgctgatgag aaggccaaag
     3601 agggccggtg ttgtccgggt gatggtgacg gtataggtgg tttcggtaaa attttcggcc
     3661 acaacccgga tggagaccgt gttgacaccg actgcaagtg gaattgccag gcttgaacct
     3721 gaagccacct gctgattgtt gacgaagatg cgggcagcag catggctggc ggcgggtgtc
     3781 acgctggctg atgtaatatc gaaattgacg gcgcatgaat aggttgttgt cgctggtgtg
     3841 aacgagagga tattggttgg tccgaccacg agggaggaga gactggagtc accattctca
     3901 ctccccgtca ggagaaccgc cgagactgtg taggtaaaca cggacaagcc atcacggtcc
     3961 gtcacccgta ccgggacccg ggttgttccc gttgcgggga cgatgggttg tgacgccatt
     4021 ccggagagga cggtctcgcc gttaaccgta atcctggaag tgctgctgcg tgaaacgggg
     4081 gtaatggtga acgaagccgt tcccgccggg actccgatca tataattggt acgttcgatt
     4141 ccaaaagccg ggacgagtgc ggtggagagt gacgtcgaaa ggcctgagag aaatggatca
     4201 ggtaaaaatt tccgttcgta ttggtccggc agctcgccgc tgt
//
LOCUS       DAOSHE010000147         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19098_length_4200_cov_3.323522, whole genome shotgun sequence.
ACCESSION   DAOSHE010000147 DAOSHE010000000
VERSION     DAOSHE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19098_length_4200_cov_3.323522"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLD82_05220"
     CDS             complement(<1..247)
                     /locus_tag="PLD82_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG64819.1"
                     /translation="MNSIVKLDTVSKIYRRGREEVQAIKNVSFEIFEGDQIAICGPSG
                     SGKTTLLNLAGCMDHPTTGTISVEGKEVGQASESVLAD"
     gene            complement(240..1415)
                     /locus_tag="PLD82_05225"
     CDS             complement(240..1415)
                     /locus_tag="PLD82_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64820.1"
                     /translation="MKLAKLAGNNLRRTPARSVLTAVSVLIATATLTVVLSVDRGYSK
                     AVERDLVEKTGVHLYITKEGCPIEAAAVIAQGGLSPLYVEENLVDKVRAIPEVAAVLP
                     FQLFTATTKDGSRTDIFMGITEEIRAIRPDWIITNGGWFSAENSVILGSQIAQIENVA
                     PGDKLYSEKYDKEFVVAGILAQNWGQDDGTIFLPLKTAQEMIERSGKLSAIAIKLKDI
                     SRLQDVTLRLRGDLPKEYYVLGSKELSDGILGFFSATRVIMFVMVVVAFGVSVFGILN
                     TMLMAVMERRREMAYLKCVGAKRRDILGLVTLESLILCSAGAIAGAGIGLALMPAADH
                     LLRGLLIAYIPAGSITDPTLPLISLSILLTVAVGVVCALYPAWKAASIVPMEVLRNE"
     gene            complement(1390..>1876)
                     /locus_tag="PLD82_05230"
     CDS             complement(1390..>1876)
                     /locus_tag="PLD82_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64821.1"
                     /translation="DRSTLTPESGGTRKDIIMSRYHSISNLSRLFTALLTGALLLLAA
                     CSSGEVIGTPAPAETVPTALSEILERPADFEGKTVVLRGQLSSQCKSLCEFTYTEKSR
                     SVTVFMGDLKAPRIRQGQQLRVTAKVHKGDEQVVLTATGLELVEGKVEGDSNETRKTG
                     R"
     assembly_gap    1877..1976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1977..2182)
                     /locus_tag="PLD82_05235"
     CDS             complement(<1977..2182)
                     /locus_tag="PLD82_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64822.1"
                     /translation="MCFLALAVFAAALPSPVCAGRLTQDAHSLPCVETPLFGSTTPCP
                     CPDCATDSHCPDCMSDETHSRQVP"
     gene            2427..2876
                     /locus_tag="PLD82_05240"
     CDS             2427..2876
                     /locus_tag="PLD82_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64823.1"
                     /translation="METRVYYVHAGAKTAYTVVGVLVSLLVITIPLGIWIIVASRRAR
                     VTLDERGFRVKMFGTISLPWDQIARVGLLRISVQGGGVAGILVKKKVGGDEAINLCLQ
                     SRKGRTRHCIISMYQNWEDIVEHVAQRTGRQCETLSRGFSGPKWPEG"
     assembly_gap    3042..3141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3142..3321
                     /locus_tag="PLD82_05245"
     CDS             <3142..3321
                     /locus_tag="PLD82_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64824.1"
                     /translation="IPKKEADRIFDAVAEAAQVSLPGKGFIYMLDCIKAMTYFPKPEK
                     KRAEKADKKTVTKGR"
     gene            3324..>4200
                     /locus_tag="PLD82_05250"
     CDS             3324..>4200
                     /locus_tag="PLD82_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOG64825.1"
                     /translation="MREDRIRVGFVNGVRILAPWASAKFMEQVRAVIPLVALMFVFQL
                     LVLQSGIDHALTISGGILAVIIGLAFFMEGLRLGLMPIGEVLGSRLPVKAPFVVVLVI
                     LFVLGIGVTLAEPAIGTLQSVGASVDPAKAPFLYSLLKDHAAVLALAIGLGVGIAAVL
                     GTLRFVYGFSLSRYLFPVVGLTTVLTLIAAVTPGVREIIGLAWDSGAITTGPVTVPLV
                     LALGIGIARVLAKKHDSGMGGFGIVTLASLFPIIGVLLLGFVLHLTGAIDPAQVRAVT
                     QTPLSGADLVTAIAIA"
BASE COUNT          798 a         1077 c         1238 g          887 t
ORIGIN      
        1 tgtctgccag aacagactcg ctggcctgtc caacctcctt gccctcgacc gagatggttc
       61 cggtcgtcgg gtggtccatg cagcccgcca gattgaggag ggttgtcttg cctgaaccgg
      121 agggaccgca gatcgcgatc tggtctccct cgaaaatctc gaagctgacg ttcttgatag
      181 cctggacttc ctcgcgtccc cggcggtaga tcttgctgac agtatccagt ttgacgatgc
      241 tattcatttc ggagtacctc catggggacg attgaggcag ccttccaggc ggggtagagg
      301 gcgcagacga cacccacggc caccgtcagg aggatcgaga gggagatgag ggggagtgtc
      361 ggatcggtga tcgaaccggc agggatgtag gcgatcaaaa gcccacgcaa caagtggtcc
      421 gccgccggca tcagggctaa accgatccct gctccggcga tggcgccagc actgcagagg
      481 atcaggcttt cgagtgtgac cagaccaagg atgtcgcgcc gtttggcacc gacgcacttg
      541 aggtaggcca tctcgcgcct gcgctccatg acggccatca gcatggtgtt gaggatcccg
      601 aagacggaga caccgaaggc cacaacgacc atgacaaaca tgatcacgcg tgttgccgaa
      661 aagaacccga ggatgccgtc gctcagttcc ttcgagccga ggacatagta ctccttggga
      721 agatcgccgc gcagccggag ggtcacatcc tgcaggcgcg agatgtcctt gagcttgatg
      781 gcgatggccg agagttttcc gctgcgttcg atcatctctt gggcagtctt gaggggcaaa
      841 aagatcgtcc cgtcatcctg tccccagttt tgggccagga taccggccac aacaaactcc
      901 ttgtcgtatt tttcagaata gagtttgtcg cccggtgcga cgttttcgat ctgggcaatc
      961 tgggagccca ggatgaccga gttttcagcg ctaaaccagc ccccgtttgt gatgatccag
     1021 tccggtcgga tcgcgcggat ttcctcagtg atgcccatga agatgtctgt ccggcttccg
     1081 tcctttgtag ttgcggtaaa gagctggaag gggaggacgg ccgccacttc gggaatggcg
     1141 cgcaccttgt caaccaggtt ctcctcgaca tacagcggac tcagtccgcc ctgggcaatg
     1201 accgcggccg cctcgatcgg gcagccttcc tttgtgatat agagatgcac gccggtcttt
     1261 tcgaccaggt cgcgttcgac cgccttgctg tagccgcggt ctaccgagag gaccaccgtc
     1321 agggtggccg tggcgatcag gacactgaca gcagtcagga ccgagcgggc cggggtccgg
     1381 cgcaggttgt taccggccag ttttgcgagt ttcattgctg tctccttcca cttttccctc
     1441 gaccagttca agtccggtgg cggtcaggac gacctgctcg tcacccttgt ggactttggc
     1501 ggtcacgcgc aactgctgtc cctggcggat acggggggcc ttgaggtctc ccatgaagac
     1561 tgtgacagag cgggactttt cggtataggt aaactcgcaa agcgatttgc actgggacga
     1621 gagctggccg cgcaatacga cggtcttgcc ctcaaagtcg gctggacgct cgaggatctc
     1681 tgacagggct gtgggaacag tctccgccgg ggcaggggtg ccgatgactt cgccggacga
     1741 acaggccgcg aggaggagga gggctcccgt caggagcgcc gtgaagagac gtgacaggtt
     1801 tgatatagaa tggtatcgtg acatgataat gtcctttcgt gttcccccgg attcgggggt
     1861 gagtgtgctt cggtctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg
     1981 ggacctgacg ggaatgcgtt tcgtcactca tgcagtccgg gcaatgcgag tcggtggcgc
     2041 agtccgggca ggggcagggt gttgtgctgc caaagagagg tgtttctacg caggggaggg
     2101 agtgagcgtc ctgggtcagt ctgccagcgc agacaggtga gggaagggcg gcagcaaaga
     2161 ccgcaagtgc gaggaagcac atgcttagcg atatcatcct gctttgcccg tttctattca
     2221 taccatattt ataggaaaaa agaaggcagg atgcaagtct ttttggaaaa aatgctgtgt
     2281 attgcgcgtt caatcgggcg ctattcgggc taaccaccac attttcatga aaatgtcatg
     2341 gcactccggg ccacgtatta cacatctgta ctgacactcc cggagtgttg tatactggaa
     2401 ccacaacatc aatccaggag gtgtgcgtgg aaactcgagt ctattacgtt catgccgggg
     2461 caaaaacggc gtataccgta gtcggcgtcc ttgtatctct gcttgtcatt accattccgc
     2521 ttgggatatg gattatcgtt gcctcccgtc gggcccgtgt cacgctggat gagcggggct
     2581 ttcgtgtcaa aatgttcggg accatttccc tcccctggga ccagatcgcc cgggtcggtc
     2641 ttctgcgcat cagtgttcag ggtggggggg tggccggtat cctggtcaag aaaaaggtgg
     2701 gaggggatga ggcgatcaac ctctgcctgc aaagcaggaa aggcaggacg cggcactgca
     2761 ttatctcaat gtaccagaac tgggaagaca tcgtggaaca tgttgcacag cggaccggcc
     2821 ggcagtgcga aaccctctcc cgtggttttt ccgggccaaa atggcctgag ggctgaggcc
     2881 cgcagcagga tcgtcatcag cagtatcagg tgtcccgggt actcatgaca caattatgat
     2941 gcgtccctgg gatagtgtgc tgaactgatc tacaggattt cgtaccataa atatggaaga
     3001 aaggacgctg gattatccgg aaaagatgcc gataatcggg annnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn natccccaaa aaagaggcgg acaggatctt tgatgccgtg
     3181 gccgaagcgg cccaggtcag tcttccgggc aagggcttta tctacatgct ggactgcatc
     3241 aaggcgatga cgtattttcc caaacccgaa aaaaagcgtg ctgaaaaagc ggacaaaaaa
     3301 accgtgacca aaggacgctg atgatgcggg aagacaggat cagagtcgga tttgtcaacg
     3361 gtgtgcgtat cttggcacca tgggcctcag caaaattcat ggagcaggtc agggcggtga
     3421 ttccccttgt cgcgctgatg ttcgtgtttc agctccttgt cctccagtca gggatcgacc
     3481 acgcgctgac catctccgga gggattctcg ctgtcatcat cggcctggcg ttttttatgg
     3541 aaggactccg gctggggctg atgccgatcg gtgaagtcct cggctcgcgc ctgccggtca
     3601 aggcgccctt tgtcgttgtg ctggtcatcc tgtttgtttt gggaattggc gtcaccctgg
     3661 ctgagccggc tatcggaaca ttgcagagtg tgggtgcctc ggttgatccg gccaaggcgc
     3721 catttttgta ttccctgctc aaagatcatg cggccgtgct ggcacttgcc atcggactgg
     3781 gggttggcat tgccgcggtg cttggaacct tgcgttttgt gtacggtttt tccctcagcc
     3841 gctatctttt tcccgttgtc ggattgacga cggtcctgac gctcattgcg gcagtgacac
     3901 ccggggttcg ggagatcatc ggcctggcct gggattcggg agccatcacg accgggccgg
     3961 tcacggttcc ccttgtcctg gccctgggga tcggaatcgc ccgcgtgctg gccaaaaagc
     4021 atgactcggg aatgggcgga tttggaatcg ttaccctggc atccctcttc cctataatcg
     4081 gggtgctcct cctgggcttt gtcctgcacc tgactggagc gattgatccg gcccaggtca
     4141 gggccgtcac gcagacacca ctcagcggtg cggaccttgt gaccgccatt gccatcgcct
//
LOCUS       DAOSHE010000148         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19133_length_4196_cov_5.229172, whole genome shotgun sequence.
ACCESSION   DAOSHE010000148 DAOSHE010000000
VERSION     DAOSHE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19133_length_4196_cov_5.229172"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..540
                     /locus_tag="PLD82_05255"
     CDS             325..540
                     /locus_tag="PLD82_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64826.1"
                     /translation="MGETPEVDALVEDLVLALEDARRDLEAALTDREHAQNDDMAALV
                     NEEIDAISEEISLLESELETARGREPG"
     gene            complement(543..1268)
                     /locus_tag="PLD82_05260"
     CDS             complement(543..1268)
                     /locus_tag="PLD82_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64827.1"
                     /translation="MEDGIRILVAEDEEINRKLLGKLLERHGFVQMTVESGRVALTEW
                     RNGGWDLVILDVNMPDMGGMATAKAIREEEAVGGSRRTPILALTAAVDEEERTACLAA
                     GMDAILAKPLRIETLIDLVNAFFEGRKYSLETLVKAENDDHEFAIELVRAYLKNEPAY
                     VSTMLDAVARGDAEELKKAAHKLRGALCIIRAGTPLVEIVRRLEDCGSGNKMDEAEEL
                     VPRARLALGAFSASLAAIPGASD"
     gene            complement(1284..3080)
                     /locus_tag="PLD82_05265"
     CDS             complement(1284..3080)
                     /locus_tag="PLD82_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG64828.1"
                     /translation="MQIQIVRNRLGRKITALVAIPLVAYLVVSIIEIRLTVTESETTS
                     VMRDNMRIMAGVSPVIHHLQRERGRSSMFLTGTITAEQLAAQRRDTDAARSDIGKVLA
                     DVELDKVYRNKLDACLALVDGIRSDVDGRKIDTITAQKRYSEVIAVLLEIEGRIPSMK
                     TARGLGKMMSSLLMFELAKENGGQLRAFVSGIIGLDKPIDVDMLRRMLRLHISVDEYL
                     GSPATLYTEKSRKIIDEARTSSAWLHVEEAVTRIVTRYERGKYELDAAKFFTEITAKL
                     DAINAALENDMRAIVEDIRKIDGEAQTALVITASVIALILLSVVVFAVFFIRSTTRPI
                     LTVVAEISEGTYQVKSASVELAGSSNSLAAGASQQAAAIEETSASLEEMSAITKQNSE
                     NAARSEGLMKETGRVVDDANSSMEQVVGAMREIATTGESIAAIVRTIDEIAFQTNLLA
                     LNAAVEAARAGDAGAGFAVVAQEVRHLAVRSAEAAKNTSSMLEQSIERIKVGEGLVDT
                     SQQAFVRMTDSFRKVSTMVNEISSASKEQAIGIEQITRAVTEMDKVVQQSAATSEETA
                     SAAEELSSQSAEMNASVTRLLDIVGARRTDQA"
     gene            3192..3587
                     /locus_tag="PLD82_05270"
     CDS             3192..3587
                     /locus_tag="PLD82_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG64829.1"
                     /translation="MATLRIENREQHELTVDASVSLLNLLIREGVEILNRCGGHARCG
                     FCRVTFLEGLDHTSPRTPHETRLAREAGFPPEVRLACQTRLAGNALIRIGHSATQGTR
                     QAPPPESTETPLPSGEGLPGGKIPGPLSE"
     gene            complement(3516..>4196)
                     /locus_tag="PLD82_05275"
     CDS             complement(3516..>4196)
                     /locus_tag="PLD82_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64830.1"
                     /translation="PRPIQGRLVVNDIEDFIRNEKIDFDRPEDCFRLQARFCGECGES
                     CSILQALQKFVHVIPRLPEVAGKRTVPVPAIDLSTEMDRETILRILGENRSVNETVDL
                     AFHAARLPGDWFEPYWHAAFRRNPVSLELSSSLGDLEAVVAELRSMDESSIYEEERIA
                     QPDEVWNFRTGDGFEKAVSLVNIALARGEGPLVLRVEGEMVRVLLGERTWDFATGKTL
                     SRGEWRFG"
BASE COUNT          878 a         1365 c         1063 g          890 t
ORIGIN      
        1 ccatggtgca tccattccgc gacctgacgg ccccagctcg cctgagggtg ttcatcaaaa
       61 aaaaaaccac ccaatctgag cggttttgtt ggcaactcag tgcaccatgg gtgcctccat
      121 gtccttactg aacgctttcc gcactatgga gcgcggcgat ggcgcccttg aagtcatcgc
      181 aatttctcct ggaccgcacc atgacagcga accacggctt ggcaaatcga tacatcgaat
      241 acgcttgacc ccttgccgtc cacaaaaaaa aatgctattc tgcaaagaag gaatccggct
      301 gatgtcccgc caaggagcga caggatgggc gaaacacctg aagtggatgc acttgttgaa
      361 gatcttgtac tggcccttga agatgcccga cgcgatctcg aagcagctct gaccgaccgg
      421 gagcatgccc aaaacgacga catggcggcc cttgtcaatg aagaaatcga cgccatcagc
      481 gaagagatct ccctgctgga atccgaactc gagaccgccc ggggaaggga accgggttga
      541 tcctagtcac tcgcaccggg gatggccgcg agactcgctg aaaaagcacc cagagccagt
      601 ctcgctctcg gcaccagttc ttccgcttca tccattttat tccccgaccc acagtcttcc
      661 agcctgcgca caatctccac cagcggcgtt ccagcccgga tgatgcaaag cgctcctctg
      721 agtttatggg cagctttttt cagttcttcc gcatccccgc gtgccacggc atccagcatg
      781 gtcgagacat aggcaggttc gtttttcaga taagcgcgca ccagctcaat cgcaaattcg
      841 tgatcgtcat tttcagcctt gaccaaggtc tcgagggaat acttccgccc ttcaaaaaaa
      901 gcattcacca gatcgatcag tgtctcaatc ctcagcggct tggccagaat cgcatccatc
      961 ccggcggcca gacaggcagt ccgctcttcc tcgtcaaccg cagccgtcag ggccagaatc
     1021 ggcgtgcgcc tgctcccccc cacagcttcc tcttcccgga tggccttcgc agtggccatc
     1081 ccgcccatat cgggcatatt cacgtccaga attacgagat cccaaccgcc attccgccac
     1141 tccgtcagcg cgacacgccc cgactcgacc gtcatctgca caaacccgtg ccgttccagg
     1201 agtttcccta aaagcttgcg gttgatctcc tcgtcctcgg ccaccaaaat ccgtattcca
     1261 tcttccatga aacctccagc ccctcaggct tgatcggtac gccgtgctcc gacgatgtcc
     1321 aggagtcgtg tcacggatgc attcatctcg gcggactgac tggaaagctc ctcggcagca
     1381 ctggctgttt cttccgaggt cgcggcgctc tgctgtacca ccttgtccat ctcggtaacg
     1441 gcacgcgtaa tctgctcgat cccgatggcc tgctcctttg atgccgagga gatctcgttg
     1501 accatcgtcg agaccttgcg gaaactgtcc gtcatccgca caaaagcctg ctgggacgta
     1561 tccacaagcc cttccccgac cttgatccgt tcgatagact gctcaagcat gctggatgtg
     1621 ttttttgcgg cttctgccga gcgcacggcg agatgccgga cttcctgtgc gactacggca
     1681 aaccccgcac cggcatcccc ggcccgggct gcctcgaccg cggcattcaa agccaaaaga
     1741 tttgtctgga aggcaatctc gtcgatcgtc cggacaatgg cggcaatgct ctccccggtt
     1801 gtcgcaatct cgcgcatcgc tcccacaacc tgctccatcg agctgttggc gtcatccacc
     1861 acccgacccg tctctttcat gagcccctcg gatctggccg cgttttcact gttttgcttt
     1921 gtaatggccg acatctcctc gagggaagcc gaagtctcct cgatcgccgc ggcctgctgt
     1981 gaagccccgg cagcaagaga attgctcgag ccggccagtt caaccgaggc actcttgacc
     2041 tggtaggttc cctcgctgat ctcggccaca accgtgagga tcggccgcgt tgttgaacgg
     2101 atgaaaaaca ccgcgaacac gacgaccgac aacagaatca gggcaatgac ggaagcagtg
     2161 atcaccaggg ccgtttgtgc ctcaccatca atcttgcgta tgtcttcgac tattgcgcgc
     2221 atgtcgtttt caagcgcggc attgatcgca tccagcttgg ccgtaatctc ggtaaaaaac
     2281 tttgcggcat ccagttcgta tttcccgcgt tcataccgtg tgacaatcct cgtcaccgct
     2341 tcttccacat ggagccaggc tgaagaggta cgggcctcgt cgatgatctt cctgcttttt
     2401 tcggtataga gcgtcgcggg agaaccaaga tactcatcca cgctgatatg gagacgaagc
     2461 atcctgcgca acatgtcaac atcgatcggc ttgtccagtc cgatgatccc ggagacaaag
     2521 gcccggagct ggcctccgtt ttccttggcc agttcgaaca tcaggaggga actcatcatc
     2581 tttccgagcc cgcgggccgt cttcatggag gggatccggc cttcgatctc aagcagcaca
     2641 gctatgactt cggaatagcg cttttgcgcc gttatcgtgt cgatcttccg cccatcgaca
     2701 tcactccgga ttccatccac cagtgcgaga caggcatcca gtttgttgcg atacaccttg
     2761 tccagctcaa catcggccaa cactttgccg atgtcgctcc gggcagcatc cgtatcccgt
     2821 cgctgggccg caagctgctc cgcagtgatt gtccccgtca aaaacatcga ggaccgtcca
     2881 cgctcccgct ggagatggtg gatcacgggc gagacaccgg ccatgatgcg catattgtcc
     2941 cgcatcacac tggtcgtttc ggactctgtc actgtcaagc gaatttctat tattgatacc
     3001 accaggtacg cgaccagggg gatcgcaaca agtgccgtga tttttctgcc aagccggtta
     3061 cgcacaatct ggatctgcat gctgttctcc gtcgggattc aaatactctg tcttaccact
     3121 ccagagagag tataccgtgt tttccatatg ctgtatactg cattcgcagt acgatccaga
     3181 aaggacccca catggccaca ctccgaatcg aaaaccggga acagcatgaa ctgaccgtcg
     3241 acgcttccgt ctccctcctg aacctcctca tccgggaggg cgtcgagatt ctcaatcgct
     3301 gcggagggca cgcccggtgc gggttttgcc gggtcacatt tctggaagga ctcgaccaca
     3361 ccagtcctcg caccccgcat gagacacgcc tggcgcggga agccggattt cccccggaag
     3421 tccgcctggc ctgccagacc cgcctggccg gcaatgccct gatccggatc ggccacagcg
     3481 ccacacaagg gaccagacag gcccccccgc ccgaatcaac cgaaacgcca ctcccctcgg
     3541 gagagggtct tcccggtggc aaaatcccag gtcctctctc cgagtaagac cctgaccatt
     3601 tcgccctcga ctcggagtac gagcggtccc tccccccggg ccagagcaat gttcaccaga
     3661 gagacagcct tctcgaaccc gtcacccgtc ctgaagttcc agacctcatc gggttgggcg
     3721 atgcgctctt cttcgtagat cgagctttca tccatcgaac ggagttccgc gacaacagcc
     3781 tccagatcac ccagactgga agagagttcg agactgacag ggttgcgccg gaatgctgca
     3841 tgccagtacg gttcgaacca gtctccggga aggcgtgccg cgtggaaggc gagatcgacc
     3901 gtctcgttca cactcctgtt ctcgccgagt atccgcagga tcgtctcacg gtccatttca
     3961 gtcgagagat cgattgcggg aaccggtacg gtccgtttgc cggcgacttc gggcaggcga
     4021 gggatgacat gcacaaattt ctgcaaggcc tgcaggatac tgcaactctc cccacactcc
     4081 ccgcagaacc tcgcctgcag cctgaagcaa tcctcgggcc ggtcgaaatc gatcttctcg
     4141 ttccggataa agtcctcgat atcattgacc acaagccgtc cctggattgg ccgggg
//
LOCUS       DAOSHE010000149         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19305_length_4170_cov_4.046173, whole genome shotgun sequence.
ACCESSION   DAOSHE010000149 DAOSHE010000000
VERSION     DAOSHE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19305_length_4170_cov_4.046173"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLD82_05280"
     CDS             <1..666
                     /locus_tag="PLD82_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG64831.1"
                     /translation="AAMGVRRAASLADLAKASDVVSVHVAATAETKNIIDAKFFDAMK
                     KGAIFVNTSRGSVVDYDALKQAIQEKGIRAGLDVFPGEPAGSEADFTSEVANLDCVAS
                     APHIGASTEQAQMAIAMETVRIIECMLKTGEIPNVVNLCGETSARTILEIRHENKPGV
                     LAHVFNLLSQSSINVEQMENLIYTGEAAACARIHCSTGIDDSLAAKIRDKAEIISLVV
                     KNP"
     gene            932..2875
                     /locus_tag="PLD82_05285"
     CDS             932..2875
                     /locus_tag="PLD82_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG64832.1"
                     /translation="MSVFSNMSVKNKILWPVQTILILSIFVIAFFAYQQLDAIIDDLM
                     QHDGESGLEQVMSGIKRTDEGIAAMKNALNKEYLRQTKMIRQIIEMDPGVLAPAQMKR
                     LQETTGVSELHVTDGRGVLSWGSVPVFYGFDFTTQDQTRPFLPGLTNTSFEMAQDPQP
                     RGTTKEIFQYITVARRDRPGLIQVGVQPRELQDMMEKNDLQSLIRTAKFGMGGWVFVM
                     DEKGVCLAHPQAKRVGSKLAETDWGKTILAKKTGVIETVLDGVPVLATIGVSGTRIVA
                     AILPIEPYQEPLSDFRLKLIIAILLSLFISSYIVYIIAKRFIMLPVFAALDSLREAAL
                     NVESASDELAASSANLAKGSSQQAAALQETTSSLAEMASMSKQAAELTCGANELMNEN
                     LEKSGQALKKLVELNKSMSQIEQDNGQIAKIIKTIDEIAFQTNLLALNAAIEAARAGT
                     AGAGFAVVADEVRNLAMNTTEAAKNTQTLLNNTVQRVSHAAQSIKLMNADFEGIVESA
                     TLIGEKTSAITDASRQQARGIEQLSTTSHEIENVTQQIAASSEETAATAKELEGNSKT
                     VRARITELSHIVAGVHNSELFEHEYYTQRGVSHDTPAARAPEAEVEAPKQIPASPTPQ
                     HARIGSVSPEQVIPLDDGDIRDF"
     gene            2887..3963
                     /locus_tag="PLD82_05290"
     CDS             2887..3963
                     /locus_tag="PLD82_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64833.1"
                     /translation="MQSLDGLSDFIHGPPVRHIPAGDGFSLVSLVPAALPGLNVFVTP
                     CGGQDRSPSKILSACLLGPVDQAVARLACTTALHLLLSVREALPCTALLRALELLKES
                     GLGPPDLRIDAGVAVLDCRQSLLRLAHTTSCTITALRSGVQVPLQSLNARHCAGNADQ
                     TLLSGWFRFEAGDRIHWRFPDASKTGVVTLEQSIEKATPDVSKEIKPGSDTDISAWIS
                     SLADELSASLDAAGFEAAESRVRLIIEEAVLNAWKHGNRKNPSKTIRVSWRIGVDCVL
                     SVEDEGPGFDHQKIGDPRSTDNLLKLCGRGIFIIKNYAGDISWSDDGRVAMISIERAM
                     PGQQTGKDSLQCSSNLIECWKALS"
BASE COUNT          967 a         1301 c         1094 g          808 t
ORIGIN      
        1 gccgccatgg gcgtgagacg ggccgccagc ctggccgatc tcgccaaggc gtccgatgtc
       61 gtcagtgtcc acgttgccgc gaccgccgaa acaaaaaaca tcatcgacgc caagttcttt
      121 gacgccatga agaagggcgc gatcttcgtc aacacatccc gcgggtccgt cgtagactac
      181 gatgccctca aacaagccat ccaggaaaag gggattcggg ccgggctgga tgtcttcccc
      241 ggcgaaccgg ccgggagcga ggctgacttc acctccgagg tcgccaatct tgactgcgtg
      301 gcctccgcgc cccacatcgg cgcctcgacc gaacaggccc agatggcgat cgcgatggag
      361 accgtgcgca tcatcgagtg catgctcaag accggcgaga ttcccaatgt cgtcaacctc
      421 tgcggtgaga cctcagcgcg caccatcctc gagatccgcc acgaaaacaa gccgggtgtc
      481 ctcgcccacg tcttcaacct cctctcccag tcctcgatca atgtcgagca gatggaaaac
      541 ctgatctaca ccggcgaagc agcggcctgc gcccggatcc attgttccac cgggattgat
      601 gattccctgg ctgccaaaat tcgtgacaag gccgagatca tctccctcgt cgtgaagaat
      661 ccctgacaca ggggccgttt caaagccata cggggctgtc ccacaaggac ggcctctttt
      721 ttttgctgaa catcctcagg aggagagcgg atctgcctgt tccgtgtcac gcctgtaccc
      781 caagagaggg cagacacccc gtcacgcaag gctgtgtccc cgtgcgggtt cacacctccg
      841 cgttgagacc tttcaaaaat tcaaaaatgg gtatacactc tccgataatc gatggggttt
      901 gtttttttga ggactgcgca aggagtgcaa catgagcgtg ttttccaata tgagtgtcaa
      961 aaacaagatc ctctggccgg tccagacgat cctgatcctt tcgatctttg tcattgcgtt
     1021 ttttgcctat caacagctcg atgcgattat cgatgatctc atgcagcatg acggcgaatc
     1081 cgggctggag caggtcatgt cgggcatcaa gcgtacagac gaaggcattg ctgccatgaa
     1141 aaacgccctc aacaaggaat acctccgcca gaccaagatg atccgccaga tcatcgagat
     1201 ggatcccggt gtccttgctc cggcacagat gaaacgcctc caggagacca ccggtgtctc
     1261 tgaactccat gtcaccgatg ggcggggtgt gctctcatgg ggaagcgtcc cggtatttta
     1321 cggttttgac tttaccaccc aagaccagac cagaccgttt ttgcccggct tgacaaatac
     1381 cagctttgaa atggcccaag atccccaacc ccgtgggacg accaaagaga tcttccagta
     1441 catcacggtc gcccggcgtg accgtcccgg cctgatccag gttggagtcc agccgaggga
     1501 actccaggac atgatggaaa aaaatgacct ccagagcctg atccggaccg ccaagttcgg
     1561 catggggggc tgggtctttg tgatggatga aaaaggtgtc tgtctcgcac atccccaagc
     1621 caaacgggtt gggtccaagc tcgccgaaac cgattggggc aagacaatcc tcgcaaaaaa
     1681 aacaggtgtg atcgaaaccg tactggacgg ggtcccggtt ttagcaacca ttggcgtgag
     1741 tggcacacgt atcgtggctg ccatcctccc gattgaaccc taccaggagc cactctccga
     1801 tttccgtctt aaactgatca ttgccatcct gctctccctc ttcatctcct cgtacatcgt
     1861 ctatatcatc gccaagcgct tcatcatgct tccggtcttc gccgcgctcg acagtctccg
     1921 cgaagcggcc ctcaatgtcg aaagcgcttc ggacgaactg gccgcatcca gtgcgaatct
     1981 ggccaagggt tcatcccagc aggccgccgc tctccaggaa acaaccagct ctttagccga
     2041 gatggcctcc atgtccaagc aggcggccga gctgacatgt ggcgccaacg agttgatgaa
     2101 cgagaacctc gaaaaatccg gacaggccct caaaaaactg gtcgagctca ataagagcat
     2161 gtcccagatc gaacaggaca acggacagat agccaaaatc atcaaaacga tcgatgaaat
     2221 cgccttccag accaatctcc tcgccctgaa tgccgcgatc gaggccgcca gagccggaac
     2281 cgccggcgcc ggatttgccg ttgtcgccga cgaggtccgc aatcttgcga tgaacaccac
     2341 cgaagccgcc aagaacacgc agaccctcct caacaatacc gtgcagcgtg tctcacacgc
     2401 cgcgcagtcc atcaagctga tgaatgcgga tttcgaagga atcgtcgaat cggcgactct
     2461 gatcggagaa aaaacatccg ccatcaccga tgccagcagg cagcaggccc gggggattga
     2521 acagctctcg accacctccc acgagatcga aaatgtcacg caacagattg cggcaagttc
     2581 cgaggaaaca gcagccaccg ccaaggagct ggaagggaat tccaaaacag tccgtgcacg
     2641 catcacggaa ctctcccaca tcgtggccgg tgtccataat tccgaactct tcgagcacga
     2701 gtactatacc cagcgtggag tcagccatga cacgccggca gcacgggccc cagaagcgga
     2761 agtggaagct cccaaacaga ttcctgccag ccctacaccc caacacgccc ggatcggaag
     2821 tgtttccccg gagcaagtga tccccctgga tgatggcgac atccgggact tctaggagca
     2881 ggccagatgc agtcactcga tggcctctcg gacttcatcc atggccctcc agtacgccat
     2941 atcccggccg gagacgggtt ctctctggtt tccctcgtcc cggccgccct gcccggtttg
     3001 aatgtcttcg tcacaccctg cgggggccag gatcgcagcc cgtccaaaat cctgtcggcc
     3061 tgtcttctcg gcccggttga ccaggccgtc gccaggctgg cgtgtacaac cgccctgcat
     3121 ctcctcctgt cggtccggga agcccttccc tgcacagccc tgctccgtgc actcgaactc
     3181 ctcaaggaat caggactcgg cccgcccgat ctccggatcg atgccggcgt tgcggttctg
     3241 gactgccggc aaagcctgct gcgactggcc cataccactt cctgcaccat cacagccctg
     3301 cgttcgggag tacaagtgcc gctccagtcc ctcaacgccc gtcactgtgc cggcaatgcc
     3361 gaccagaccc tgctctccgg ctggttccgg ttcgaagcgg gagaccggat tcactggcgt
     3421 tttcctgatg catcgaaaac cggcgtcgtc acgcttgaac aatcaatcga gaaagccact
     3481 ccggatgtca gcaaagagat caaacccgga agtgacacgg acatcagcgc atggatctcc
     3541 agcctggccg acgaactctc cgccagcttg gatgccgccg gctttgaagc ggccgagagc
     3601 cgtgtacgac tgatcatcga ggaagcggtc ctcaacgcat ggaagcacgg aaaccggaag
     3661 aacccgtcca aaaccatccg ggtcagctgg agaatcggcg tggattgtgt cctctctgtc
     3721 gaagatgaag gtccggggtt tgaccatcaa aaaatcggcg atccacgcag tacggacaac
     3781 cttctcaaac tctgcggtcg gggcatcttc atcataaaaa attatgccgg agatatttcg
     3841 tggtcagatg acgggcgtgt ggccatgatt tccatcgaaa gagccatgcc cggccaacag
     3901 acaggcaaag attccttgca atgttcttcg aatctgatag aatgttggaa ggcactctca
     3961 tgaaatgcat gccaaactac ttcacccaag gagcaccaac tgtatggaga tgagtgtaca
     4021 ccagtccgga gaccaggtcc ggttcgtcct tgctggacgt atagatgaaa aagcggccga
     4081 gcaactcaag gagcgttttc atgctctgtc ccttgttggc acccgcgaaa tcgtctttga
     4141 cctcaaagat gttccccata ttggcagcgc
//
LOCUS       DAOSHE010000150         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19390_length_4157_cov_4.960751, whole genome shotgun sequence.
ACCESSION   DAOSHE010000150 DAOSHE010000000
VERSION     DAOSHE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19390_length_4157_cov_4.960751"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..3416
                     /gene="ygfK"
                     /locus_tag="PLD82_05295"
     CDS             129..3416
                     /gene="ygfK"
                     /locus_tag="PLD82_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOG64834.1"
                     /translation="MSDKFSVVPLERLLTMILNEYWETGSIFGISRENFFRPARKDAF
                     RMTRYGKTLESPIGVAAGPQTQMAQNVIVAWLTGSRYIELKTVQTLDELEISKPCIDM
                     EDAGYNCEWSQELLLRESIDEYINAWIILHILRYELGLDTTEDLGGIFNISVGYNLEG
                     ILKPNVQNFFDKMTDCSAELEEKFAKIKRIYRDVKKLGIPSRITDNITLSTMHGCPPD
                     EIEKIGRYLITEKKLNTTIKLNPTLLGPEMLRDILNNKLGFETFVPDEAFGHDLKYPD
                     ALNLIRSLTEAAKENGVAFNLKLTNTLESVNHRTVFPEKEKMMYMSGRALHPISINLA
                     ARLQTDFDGQLDISFSAGADCFNLAEIIACGIKPVTVSSDMLKPGGYLRGVQYLENLS
                     ATMKEAGAKTIDEFILAKAGGGTDVKKAALANLKKYAAEVVNNRNYKKSFIPDKSIKT
                     PRPLNKFDCIKAPCVTTCPASQEIPEYLYRASRGQYKKAFEVIMKTNPFPTITGMVCD
                     HLCMFKCTRQNYDMPLLIRDLKRFIVEQQKGDIKLKPGKKNGKRVAVVGAGPSGLSCA
                     FYLALEGFAVTVYETKTLAGGMAADAIPGFRLTDEQIAKDIGRITKLGVKIEYGQKID
                     AARFAKLREENDFVFVGIGAQVNKKMGVSGENSEGVIEALSFLSKVRRGEKLALGSSV
                     AVIGGGNSAMDAARTSFRMVGPEGKVVILYRRTLAEMPADHEEIKAVQDEKIEIIELV
                     APVSIARENGKTRVTCQKMQLGEPDASGRRRPVVIQGSEFTMDFDTVIPAIGQDTVYD
                     FVNADDLKTDPETLETKLPKVYAGGDAVHGPLNLITAIADGRKAAAHMSLLSGGKPDF
                     SATHADKGLFEYEMQKRSATRVFGAAAELSDPSDRKNFNIVISSLDEKTAREEASRCL
                     YCNDVCNVCVTVCPNRANFSYEPYSVNWRLSKIVDENGKAVAKEERAFVVAQDVQTAN
                     IADFCNECGNCETFCPTAGAPYRDKPRMCLTEESFAAEAAGFIIGKRDGKNMIRSNVN
                     GTVESLVLDGKKFIYESPALKAVLKVKDFSVISAELQNGAKEAELRQAATMAVLLKSL
                     ADRYLYR"
     gene            3551..>4157
                     /locus_tag="PLD82_05300"
     CDS             3551..>4157
                     /locus_tag="PLD82_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG64835.1"
                     /translation="MKCEYSFNPYQKEEPDWSDPFFDFLKSTDMPEFHRSIDGYAETP
                     LVPMPKLAAEIGVGQILVKDESHRYGINAFKGLGASYAIYRFLKGQWEKKFGEGFSPA
                     SFKDPTTMQKLGSYTFCAATDGNHGKAVAWTANKLKQRAIIYMPDNSAQARIDHITKE
                     NAEVVLVPGTFDDCVAKCASDAEVNGWQVIGDTAYPGYMEIP"
BASE COUNT          986 a         1194 c         1149 g          828 t
ORIGIN      
        1 gaggatgttc accaaaaaaa gcaccataac gatgccgacc cgtgaaacgg ggcaaaaatg
       61 tgtacatttg acgggcggta tgataggatg acgccatccg catcaatcca acgattccgg
      121 gagtagacat gtccgataaa ttttccgtag ttccgcttga acgcctcttg accatgattc
      181 tcaacgaata ctgggagaca ggcagcattt ttggcatttc cagagaaaat tttttccgtc
      241 cggcgcgcaa agatgccttc cgcatgacgc gctacggcaa aacgcttgaa tcccccatcg
      301 gagtggcggc cggtccccag acccagatgg cccaaaacgt gattgtggcc tggctgaccg
      361 gatcacgcta tatcgaactc aagaccgtcc agaccctcga cgagttggag atctccaaac
      421 cctgcatcga catggaggat gctggctata actgcgagtg gtcacaggag ctccttctca
      481 gggaatcgat tgacgaatac atcaatgcct ggatcattct ccatatcctc cgctacgagc
      541 ttggccttga caccaccgag gaccttggcg ggattttcaa catcagtgtc gggtacaatc
      601 tcgaggggat cctcaagccc aatgtccaga acttctttga caagatgacg gattgttctg
      661 ccgagctcga ggaaaagttt gccaaaatca agaggatcta ccgggatgtc aaaaagctgg
      721 gcatcccctc acggatcacc gacaatatca cgctttccac catgcacggc tgcccgccgg
      781 acgagatcga gaagatcggc cgctacctga ttaccgagaa aaaactcaac acgaccatca
      841 agctcaatcc gaccctgctc ggcccggaga tgttgcgcga catcctcaac aacaagctcg
      901 gttttgagac ctttgttccg gacgaggcct ttggccacga cctcaagtat ccggatgccc
      961 tcaacctgat ccggtccctg accgaggcag ccaaggaaaa cggtgtcgcg ttcaacctca
     1021 agctgaccaa taccctcgag agtgtcaacc accgtacggt cttccccgaa aaggagaaga
     1081 tgatgtacat gagcgggcgg gccctccatc cgatctccat caatctggcg gccagactcc
     1141 agaccgattt tgacggacaa ctcgacatct ccttttcggc tggggcggac tgcttcaacc
     1201 tggcggagat cattgcctgc ggtatcaagc cggtcaccgt cagttccgac atgctcaagc
     1261 cgggtgggta tctccggggc gtccagtatc ttgaaaacct gtccgccacc atgaaggagg
     1321 ctggcgccaa aacgattgac gagtttattc tggcgaaggc tggtggcggg acggatgtca
     1381 aaaaggccgc cctggccaat ctcaaaaaat acgcggcaga ggtcgtcaac aaccgcaatt
     1441 acaaaaaaag ctttattccg gacaagtcga tcaagacacc ccgtccgctc aacaaattcg
     1501 actgtatcaa ggcaccctgt gtcactacct gtcccgccag ccaggagatt cccgagtatc
     1561 tctatcgggc ctcccgagga cagtacaaaa aggcctttga agtcatcatg aagaccaatc
     1621 ccttccccac gattaccggt atggtctgcg atcacctctg catgttcaaa tgcacgcgcc
     1681 agaattacga tatgcctctt ctgatccgcg atctcaagcg ctttatcgtc gaacagcaga
     1741 agggcgacat caagctcaaa cccggcaaga aaaacggaaa aagggtggcc gtcgtcgggg
     1801 ccggcccgtc aggcctgtcc tgcgccttct acctcgctct cgaagggttt gccgtcaccg
     1861 tctacgagac caagacactc gcgggcggca tggcggccga cgccattccc ggattccggc
     1921 tgaccgacga gcagattgcc aaggacatcg ggcgcatcac aaagcttggc gtcaagatcg
     1981 agtacgggca gaagatcgat gccgcacggt ttgcgaaact ccgcgaggaa aacgattttg
     2041 tctttgtcgg gatcggcgcc caggtcaaca agaagatggg agtctccggc gagaacagcg
     2101 agggtgtcat cgaggcgctg tccttcctct ccaaggttcg ccgcggggag aagctggccc
     2161 ttggttcatc ggtggccgtc atcggtggtg gcaacagcgc catggatgct gcccgcactt
     2221 ccttccgcat ggtcggtccc gagggcaagg tcgtgatcct ctatcggcgc accctggccg
     2281 agatgccggc cgatcacgag gagatcaagg ctgtccagga cgagaagatc gagatcatcg
     2341 aactggttgc ccctgtttcg atcgcccgcg aaaacggaaa gacccgtgtc acctgccaga
     2401 agatgcagct tggcgagccg gatgcctcgg gccggcgccg ccccgtggtg atccagggga
     2461 gcgagtttac catggacttc gatacggtca ttccggccat cggtcaggac acggtctacg
     2521 attttgtcaa tgcggacgat ctcaagaccg atcccgagac actcgagaca aaacttccca
     2581 aggtctacgc cggcggtgat gcagtccacg ggccgctcaa cctgattacg gcgattgccg
     2641 acggacgcaa ggctgcggct cacatgtccc ttctctctgg cgggaagccg gatttctcgg
     2701 ccacccatgc cgacaaggga ctctttgagt acgagatgca aaaaaggagt gcgaccaggg
     2761 tctttggtgc cgcagcggaa ctgagtgatc catcggaccg caagaatttc aacatcgtga
     2821 tctcgtccct cgacgagaag acggcccgcg aggaagcatc ccgctgtctc tattgcaacg
     2881 atgtctgcaa cgtctgtgtg acggtctgtc ccaaccgggc caatttcagc tatgagccat
     2941 attcggtcaa ctggcgtctc tcgaagatcg ttgacgaaaa cggaaaagcc gtggccaaag
     3001 aagagcgggc ctttgtcgtt gcccaggacg tccagacggc caatattgcc gatttttgca
     3061 acgaatgcgg aaactgtgag accttctgtc cgacggccgg tgcgccctat cgggacaagc
     3121 ccaggatgtg tctgaccgaa gagagctttg ccgccgaggc tgccgggttc atcatcggga
     3181 agagggacgg gaagaacatg atccggtcca atgtcaatgg aacggtcgag tcactcgtgc
     3241 tggacgggaa gaagttcatc tacgagtccc cggcgctcaa ggccgttctc aaggtcaagg
     3301 acttttcggt catctctgcc gagttgcaga atggtgccaa ggaagcggag ttgcggcagg
     3361 cggccaccat ggcggtcctc ctcaagtccc ttgcggacag gtatctctat cgctgatgct
     3421 gatcgagccg gcgaagcccg tcatccgtaa ccggtgacgg gcttcgttcg tagactgtgt
     3481 tgatggtgta cagacagccg ggtgttccat cggggatgcg cggttcaaaa aaaaggaaga
     3541 gtgacacata atgaagtgcg aatacagttt caacccgtac cagaaagaag aaccggactg
     3601 gagtgatccg ttttttgatt ttttgaagag tactgatatg cccgagttcc accgctccat
     3661 cgacggatat gccgagaccc cgctcgtgcc catgcccaaa ctcgcggccg agatcggcgt
     3721 cggccagatc cttgtcaagg acgaatcaca ccgctacggg atcaatgcct tcaagggtct
     3781 gggcgcctcc tatgccatct accggttcct caaggggcag tgggaaaaga aatttggcga
     3841 agggttttca ccggccagct tcaaggatcc cacaaccatg cagaagctcg gatcctatac
     3901 attttgcgct gcgacggacg gcaatcacgg aaaggctgtt gcctggaccg ccaacaaact
     3961 caagcagcgc gccatcatct acatgccgga caactcggcc caggcccgga ttgaccacat
     4021 caccaaggaa aatgccgagg tcgttctcgt tcccgggacc tttgatgatt gcgtggcaaa
     4081 gtgcgccagt gatgccgagg ttaatggctg gcaggtcatc ggggataccg cctatccggg
     4141 atacatggag ataccgc
//
LOCUS       DAOSHE010000151         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19541_length_4135_cov_4.419608, whole genome shotgun sequence.
ACCESSION   DAOSHE010000151 DAOSHE010000000
VERSION     DAOSHE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19541_length_4135_cov_4.419608"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..577
                     /gene="aroQ"
                     /locus_tag="PLD82_05305"
     CDS             128..577
                     /gene="aroQ"
                     /locus_tag="PLD82_05305"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011934874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOG64836.1"
                     /translation="MRTILLVNGPNLNLLGERELGIYGRATLPEIVTGLRAQALDNGY
                     TLLDRQSNHEGVLVDFIQEHGAGAAGLIINAGALTHTSIAIRDAILARRLRFVEVHLS
                     NIHAREEFRHHSWLSDKAEGVIVGLGPDGYRYALDWLIQHPAEEAKQ"
     gene            574..1422
                     /locus_tag="PLD82_05310"
     CDS             574..1422
                     /locus_tag="PLD82_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64837.1"
                     /translation="MKRRKAILAGVLLAAVCVSMLAGIASGDARTVRDVSALRQVPDE
                     GGARIEEAMRNSPTVMQGVAALKAGALPAAVAALGESIAEDGNTGAVARYLYAEALVR
                     MGRPANALYYYNEFMSRWPGHPLADDVLVGIALVYLESGRDGDAESWLRRVITDCPQG
                     DRLPLARLLLVGNGSGRGASSDRESSDPSMNSSRSADKLDTGELSLRDRAKALDERES
                     RIEARERLLADAEKKLEIDRKRVLDIENTLLGRKQQLDTREKALDARATALDKREKEL
                     KDFENR"
     gene            1409..3130
                     /locus_tag="PLD82_05315"
     CDS             1409..3130
                     /locus_tag="PLD82_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64838.1"
                     /translation="MKTASLALGMLLVCTVVLSAAGQVSLKNEYIEVLVEKETGRFTI
                     RSTGGSPFTSADDNRFLLYNATPSTSYTTIALGGGSDIYQFGSDDGKFVTRPVVTNDA
                     AVAVWTVDDCEVSQIITLVKSPASEVADSASIAYRVRNLSSSQKTVGIRILLDPSLGD
                     SDGMPYQIPGHGLVESEYEFRDAAVPQYWYSYDDLEKPMVRAMGTLRGPGVLPPDRIV
                     FAAWRRLSRNPWRFLTERGESFRQSLFGGRDSAVALFYDSRPVEPSGSWTIATLYGMY
                     GVQQYKGSIWSMVLGGSASADDRLPFGITADIQNIGPAVLSNCRFSVSIPSNFSLVQG
                     VPGVATNLAVVLGRLDKNASRQMRWNLQAERGAKGTFDLYVTVAGMAGVQLHTASMKR
                     QVTIQPATSDELRSLSTNFFGPLVIEIPRTNTPVVVQTNPVFRPINLVDTRPVTNIVV
                     GRPVVEVFTIVKKPTIRTNVIGRIVDLPAMNVSLPSVRTNQTERTTGQADWRAAMRSR
                     IAVAIGKADALVARMLADERRRIDRMIDEALVALSGADSVREQVGQRVSAIEAFLDGI
                     AVRGGGR"
     gene            3130..>4135
                     /locus_tag="PLD82_05320"
     CDS             3130..>4135
                     /locus_tag="PLD82_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG64839.1"
                     /translation="MGQKLRTAHAVRAARLCRLQSVLEHPLLVVEQPHLFWLSGFSGT
                     SGAAFVTKDHAWFVTDPRYEEQARDEVAPEFTLVCKLERPEKILAGLRLHLAHPVIVL
                     EEETTTIAALKTWSKALEPARLVADPSPISGLRAIKEREEIIAVREALALTEEAMAWV
                     APMVRPGVTERELAANLELYCRTKGASGPSFDLIVASGPNSARPHGVAGSRRVQANEP
                     VLFDIGFFVDGYASDFTRVVYCGSRPPATLLALHARVLEAQNRGKAAIRAGSPAARPD
                     QIVREWFREQRVLGLYGHSLGHGVGLEIHEAPRLSWKSTERLEAGMLVTVEPGLYRTG
                     R"
BASE COUNT          798 a         1179 c         1307 g          851 t
ORIGIN      
        1 gtttttcgcg ttcgagccat gttttcgtat gcgctttctt gtcaaaaggg gtcagtctgc
       61 cgatactcac caagtacgac tgtctgtatc ttgtatcgga aaacgcgaac ggagtggata
      121 ggggaaaatg cgaaccatac ttcttgtcaa cggtccgaat ctcaatctcc ttggagaaag
      181 agagctcggc atctacggcc gtgcgaccct tccggagatt gtcaccgggt tgcgggctca
      241 agccctcgac aatgggtata cgctgctgga ccggcaatcc aatcacgaag gcgtattagt
      301 cgacttcatc caggaacatg gagcgggtgc cgccgggctg atcatcaacg ccggggcatt
      361 gacccataca tcgatagcca tccgggatgc gattcttgcc cgccggctgc ggtttgtcga
      421 ggtccatcta tccaatatcc acgcccgtga ggaatttcgg caccattcct ggctctcgga
      481 caaagccgag ggggtcatcg tcggcctggg gccggacgga tacagatatg cgcttgactg
      541 gttgatacaa catcccgcgg aggaagcaaa acagtgaaaa gacggaaagc aattctggcc
      601 ggtgtccttc ttgcggcggt ctgtgtttcc atgctggccg gtatcgccag tggcgatgcc
      661 cggacggtca gggatgtctc ggccctgaga caggttcccg acgaaggcgg tgcccgtatc
      721 gaagaggcga tgcggaattc ccctacggtg atgcaggggg tggcggcact caaggcgggg
      781 gcccttccgg cggcagttgc cgctttgggg gagtccatcg ccgaggacgg gaataccggg
      841 gccgttgccc ggtatttgta cgccgaggct cttgtgcgga tggggcggcc ggccaacgct
      901 ctctattact acaatgaatt catgagccgc tggccgggac acccgcttgc ggatgatgtt
      961 ctggtgggaa ttgccctcgt ctatctggag tctggacgtg atggggatgc cgaaagctgg
     1021 ctccgtcggg tcatcactga ttgtccacag ggggaccgcc ttccgttggc acgactcctt
     1081 ttggtcggga acggttcggg caggggcgcg tcgtcggata gggaaagctc tgatccgtcc
     1141 atgaattctt cccggtctgc ggataaactg gatacggggg aattgtcgct ccgggatcgg
     1201 gccaaggctc ttgacgaacg ggagagccgg atcgaggccc gtgagagact ccttgcggat
     1261 gccgagaaaa aactcgaaat tgaccggaag cgtgttctcg acattgagaa taccctgctc
     1321 ggccggaagc aacagcttga tacgcgcgag aaagccctgg atgcgagggc gacagcgctc
     1381 gataaacggg agaaggagct taaggacttt gaaaaccgct agtcttgctc ttggaatgct
     1441 gctcgtgtgt acggttgttc tttccgctgc cggccaagtc agcctcaaaa acgaatatat
     1501 cgaggttctt gtcgaaaagg aaacgggtcg tttcaccatc cgttcgacgg gcgggagtcc
     1561 ctttacgtct gcggatgaca accgattcct cctttacaat gccaccccgt ccaccagtta
     1621 caccacgatc gcgctgggtg gcggaagcga tatctaccag ttcgggagtg atgacgggaa
     1681 attcgtcacc cggccggtcg tgaccaatga tgccgccgtt gccgtctgga ctgtggacga
     1741 ctgcgaggtg agccagatca tcactctggt caagagtccg gccagcgaag tggccgattc
     1801 agccagcatt gcttaccgcg tacggaatct ttcgtccagc cagaagaccg tcgggatccg
     1861 catccttctg gatccctcgc tcggggacag tgacgggatg ccctaccaga ttcccggcca
     1921 tggtttggtc gagagcgagt acgaattccg ggatgcggct gttcctcaat actggtattc
     1981 ctatgatgat ctcgaaaaac ccatggtcag ggccatgggc accctgcgcg ggccgggtgt
     2041 actgccgccc gacaggatcg tcttcgccgc ctggcgccgg ctctccagaa atccctggcg
     2101 gtttttgacc gaacggggtg agagtttccg ccaatccctg tttggcggcc gggacagtgc
     2161 ggtggctctg ttctacgact cgcggcctgt cgagccgtcc ggctcatgga cgattgcgac
     2221 actctacggg atgtacgggg tccagcaata caaggggagc atttggagca tggtccttgg
     2281 cgggagtgct tccgctgatg accggttgcc cttcggcata acggccgata tccagaatat
     2341 cggaccggcg gtcctctcca actgcaggtt ctccgtttcc ataccgtcca acttcagcct
     2401 cgtgcaaggt gtccccggtg tggctactaa cttggcggtc gttctcggcc ggctggacaa
     2461 gaatgcgagc cggcagatgc gctggaatct ccaggcggag cggggcgcga aggggacatt
     2521 cgacctctat gtgacggtcg ccgggatggc gggcgtacaa ctccataccg ccagcatgaa
     2581 gcggcaggtg acgatccaac cggcgacgag tgacgaactc cgcagtctga gtaccaattt
     2641 ttttggtcca ctcgtgattg aaatacctcg gacaaatacg ccggttgtcg tgcagaccaa
     2701 tccggtcttc aggccaatca acctggtcga tacccgaccg gtcacgaata tcgtggtggg
     2761 acgtcccgtg gttgaagtct tcacgatcgt caaaaagccc acgatccgca ccaacgtcat
     2821 cggtcggatt gtcgatcttc cggcgatgaa tgtttctctc ccctccgttc gcaccaacca
     2881 gacagagcgg acgaccgggc aggccgattg gcgagcggcg atgcgcagcc ggattgccgt
     2941 cgccatcgga aaggcggatg cccttgtggc caggatgctg gccgatgagc ggagacggat
     3001 tgacaggatg atcgacgagg cgctggtggc tctctcaggg gcggattccg tccgtgaaca
     3061 ggtcggacag agagtcagtg ccatcgaggc attccttgat ggaattgcgg tacgtggagg
     3121 cggacgctga tgggacagaa gttgcggact gcccatgctg ttcgtgctgc gagactctgt
     3181 cgcctgcagt ccgtgctgga acatcccctg cttgtcgtcg aacaacccca tcttttctgg
     3241 ctctccggat tcagcggaac aagcggcgcg gcgtttgtga cgaaagatca cgcctggttt
     3301 gtgacggatc cccgctatga ggagcaggcc cgtgacgagg tcgcaccgga attcacgttg
     3361 gtctgcaagc tcgaacggcc ggaaaagatc ctcgccggat tgcgattgca tctggcccac
     3421 ccggtgatcg tcctcgaaga ggagacaacc accattgcag ccctcaagac ctggagcaag
     3481 gctctggaac cggcacggct cgtcgccgat ccgtctccga tctccgggct gcgggccatc
     3541 aaggaacgcg aggagatcat cgccgtccgt gaagcgttgg cattgaccga ggaggccatg
     3601 gcctgggtag cccccatggt gcgacccggg gtgacggagc gtgagctggc ggcaaacctc
     3661 gagctgtact gccggaccaa gggcgcatcc ggaccatcct tcgacctgat cgttgcatcc
     3721 gggcccaatt ccgcgcgtcc gcatggcgtt gccgggtcac ggagggtgca agccaatgaa
     3781 ccggtcttgt tcgacatcgg gttttttgtc gacggctatg cctccgactt cactcgtgtc
     3841 gtgtactgtg gttcccgtcc gccggccaca cttctggcgc tgcatgcccg ggtcctcgaa
     3901 gcacagaacc ggggcaaggc agcgatcagg gcggggagcc ctgccgcccg gcccgatcag
     3961 atcgtgcggg aatggttccg ggagcaacgg gtccttggtc tctatggcca ttccctggga
     4021 cacggcgtcg ggctcgagat ccacgaggct ccacgcctgt cctggaagtc gaccgagcgg
     4081 cttgaggccg ggatgctggt cacggttgaa cccgggctct accggacagg ccggt
//
LOCUS       DAOSHE010000152         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19623_length_4125_cov_3.321622, whole genome shotgun sequence.
ACCESSION   DAOSHE010000152 DAOSHE010000000
VERSION     DAOSHE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19623_length_4125_cov_3.321622"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLD82_05325"
     CDS             <1..206
                     /locus_tag="PLD82_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64840.1"
                     /translation="YTDGLTECTHPDNPDHMFEEVLVGNGFFNDPDSSVKSFAERIHA
                     EMVSFREDDSFDDDVCIICMDTG"
     gene            323..2536
                     /locus_tag="PLD82_05330"
     CDS             323..2536
                     /locus_tag="PLD82_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64841.1"
                     /translation="MKSLRIPLVLMSWFLLLACSPDARDSAEASTGLAIVGGGHLSLP
                     DLDESSLAVVPAKDGRNILYYCAVRNGKPGAWRATRLKDDLYEVPVALTNESLVNAVS
                     VTATMLPDGELAAIGLTGGGIRLYRHGHEAVTISSEGTVLSGQPIAILPPDATLGPVT
                     VGSNEIGLVVNPAKTRNLVLARLSRKAIDLWQETIPALEVRSLTTLPSDIAPGGSYVQ
                     HRFLGQNGGLLLYARTSARGDHDIHAGNCAGFEIRLERISSDRDDVSPCLVPQDQSLY
                     MASRAFAPQHTSVSFNLFRWSLEDTGIGPVGLPSSSHSATSLSASVAPSSASQSSSAL
                     ASSSATNSQSSSSEGSSSSTGPQFFTVTYVAVSAESGFAPVDPNTYAPGATVTVTNDM
                     SGELYRSGFVLIGWYDETTQKLYQPEDGTLDSTFTMPARNVTLVSKWMRLHLVGSDPM
                     NSYVINDANQEMNNGAGWNAFLYDAGIPYLAGAGSTSEAEVIFRINLNTVYHNATFIS
                     HSPTGMSLYGGTLALATYKWQNADQTRTVGYYEIDSSAVPNSETSFTNYISLASNNTP
                     GNTLTNTGIWRDASGTWVAGRSAAGIACYWDAARTMNELPSGNTGLARAIVRDGSDTY
                     IAGSGVNADYIWKNGSILYTYPGATINAITVANGIVYAVGKAGSEPAYWKNNTQYLLD
                     GTVGEATAICVKNGVMYIGGHLEQEGYYSVYWIDGGSYKAPSIKVGRVNGIHVEE"
     gene            2909..3883
                     /locus_tag="PLD82_05335"
     CDS             2909..3883
                     /locus_tag="PLD82_05335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG64842.1"
                     /translation="MLAHSACVTGSTGPEVVPRHEAVPKSGRITPKNNEAPTPEKMVK
                     ISFYYDANINGKRDAGETGLTNLRIELPACCRSLGPGSMLSPANTEVMVRIHGRVPAG
                     AGNAAGKTLTVVTVDEPRSIKMLPRCILPIGNDDLEVGLADGFLTSPLAPETVSMDGY
                     AAALRDPDAWHRRCVELYPRKWRYTPNYFYYGYRIPHGDLAGKPHHALDIGAAADTPV
                     YAAVPGVIVQPLYDWSFGIAGPHGTLYYAHIRPVVPVGTTVTRAALVGYIAKNQGEHV
                     HLELRPWPAGILRAFPGLSERDLLRSPLRGEAVVLPSFFHNRTDKPGR"
BASE COUNT          955 a         1209 c         1068 g          893 t
ORIGIN      
        1 tctataccga cgggttgacg gaatgcaccc accctgacaa tccggatcac atgttcgaag
       61 aggtccttgt cgggaacggt tttttcaacg atcccgacag ttcggtcaag tcctttgccg
      121 aacggatcca tgccgagatg gtcagcttca gagaagatga ctcatttgac gatgacgtct
      181 gcatcatctg catggatacc ggttgaaggc atggtctgga acacgggaag tgaggcatca
      241 gatattcgtt tcattcaata ggagaataca catatatttc attcttccgc cattccgcaa
      301 gacaaattac aggagtcacc cgatgaaatc actccgcata cccctggtcc tcatgagctg
      361 gtttttgctt ttggcctgtt ctcccgatgc cagagattca gccgaagcca gcaccggact
      421 ggcaatcgtc ggcggcggac atctctcgct tccggacctc gacgaaagtt cgctggccgt
      481 cgttccggca aaggatggcc gaaacatcct gtattattgc gctgtcagaa acggaaagcc
      541 gggtgcctgg cgggcaacca ggttgaaaga cgatctgtat gaagttcccg tcgcgctgac
      601 caatgagtct ctggtcaatg cggtctctgt gactgccacc atgctccctg atggtgaact
      661 tgcagcgatc ggactgactg gtggcggaat ccgtctctac cggcatggcc acgaagccgt
      721 cacgatcagc tcggaaggca cggtgctttc gggccaaccc attgccatcc tgcctcccga
      781 tgcgacactc ggtccggtca cggtcggcag caacgaaatc ggcctggtgg tcaacccggc
      841 aaaaacccgg aatctcgtac ttgctcgcct gtcacgcaag gccattgacc tctggcagga
      901 aaccatcccc gcgcttgaag tacgctcatt gacgaccctt ccgtccgata tcgcccccgg
      961 tggctcgtat gtccagcacc gtttcttggg tcagaatggc ggccttctgc tctacgcacg
     1021 cacatccgcg cgcggcgacc atgacatcca tgccggaaat tgcgcaggct ttgaaatccg
     1081 gctcgaacgc atttcaagcg atcgtgatga tgtttcaccc tgccttgtcc ctcaagacca
     1141 gagtctctac atggccagcc gggcttttgc accccagcac acatccgtct ccttcaatct
     1201 cttccgctgg agtctggaag atacaggcat cggtccggtc ggcctcccct cttcgtcgca
     1261 cagtgcgacg agcctgtcag cgtccgttgc accctcgtca gccagtcaat cttcctccgc
     1321 cctggcgtca tcgtcggcca caaacagcca gtcctcttcc tccgaaggtt catcctcatc
     1381 aaccggccca cagttcttca cagtcaccta tgtcgcggtc agtgccgaat ccggattcgc
     1441 accggttgat cccaatacct atgctcccgg cgctaccgtg acagtcacca atgatatgtc
     1501 cggcgaactg taccgaagcg gttttgtcct gatcggctgg tacgacgaga ccacacaaaa
     1561 actctaccaa cctgaagatg gcacactcga cagcacgttt accatgccgg cccgcaatgt
     1621 cacgctggtc tccaagtgga tgcggctgca ccttgtcggc agcgacccga tgaactccta
     1681 tgtcatcaat gacgcgaatc aggaaatgaa caacggagca ggctggaatg ccttcctgta
     1741 cgatgccgga ataccgtatt tggccggagc cggatcaaca tcggaagcgg aggtcatatt
     1801 caggatcaac ttgaatacag tatatcacaa tgccaccttt atctcccatt ccccaaccgg
     1861 tatgtcgctc tacggtggga cactcgcgct ggccacatat aaatggcaaa atgctgacca
     1921 aacgagaacc gtgggatatt acgaaatcga ctcgagtgcc gtaccaaact cggagacatc
     1981 ctttaccaac tacatctctc ttgcgagcaa caacaccccg ggcaataccc tgaccaatac
     2041 gggaatctgg cgggatgcgt ccgggacctg ggtcgccgga cggagtgctg ccgggatcgc
     2101 ctgctactgg gatgcagcaa ggacgatgaa cgaactgccc tcgggaaaca ccggtctggc
     2161 ccgcgccatt gtccgtgacg gcagcgacac ctatatcgcc gggagcggtg tcaacgcgga
     2221 ctatatctgg aaaaacggca gcatactcta tacatatccg ggagcgacca tcaatgccat
     2281 cacggtggcc aacggcatcg tctatgcggt cggtaaagcc ggctccgaac ccgcgtactg
     2341 gaaaaacaat acccagtatc tgcttgatgg tactgttggc gaagcaaccg ccatctgtgt
     2401 aaaaaacggg gtcatgtaca tcggcggaca cctcgagcag gaaggctact acagcgtcta
     2461 ttggattgac ggtgggtcgt acaaagcccc atcaatcaaa gtcggacgcg tcaacgggat
     2521 ccatgtcgaa gaatgaaaga tcgtcgtcct gacaaatgcc gggatccagc gatattatgt
     2581 cgaaatctta tcataccggg gcatagatct gaaaaatcct tacagccttt cactgagtgg
     2641 cacggcaaca tactgacggg cttgcctgaa acaggaacgg gtttcggttc cggtgtgatc
     2701 gtactttcgg tctgtcacac aaaccggcat gaatcggaca ttcaggaaac gaacgaatat
     2761 gtgtattcac cgccgttgtg tgatacactc tcagtccaag taccatgaaa tcgttgcaac
     2821 cagcgaggag gacagggagg tcggaatgac tcggacacga atatggaatc cacggaagag
     2881 tattttcacc ggattgctgc tgtacctcat gcttgctcat tctgcgtgtg taaccgggag
     2941 tacggggccg gaggttgttc cccgacacga agcagtgcca aaaagcgggc ggatcacccc
     3001 caaaaacaat gaagcaccca ccccggaaaa aatggtcaaa atatcgtttt attatgatgc
     3061 caacataaac ggcaagcggg atgccggcga gaccggattg acaaatctcc ggatcgaatt
     3121 gcctgcctgt tgtcgctcac ttggtcccgg ctccatgctt tctcccgcaa atactgaagt
     3181 gatggtccgg atccatggtc gcgttcctgc aggcgcagga aatgccgcgg gcaaaaccct
     3241 gacggttgtg acggtggatg agccccgcag catcaagatg ctgccacggt gcattctccc
     3301 catcgggaat gacgatcttg aagtcgggct ggctgacgga tttttgacat ctcccctcgc
     3361 acctgaaacc gtcagcatgg atggatatgc tgcagcattg cgtgacccgg atgcctggca
     3421 caggcgatgc gtggaactct atcctcgcaa atggcgctat accccaaact atttttatta
     3481 tggatatcga atcccccatg gagatcttgc ggggaagccc caccatgctc tggatatcgg
     3541 ggcggctgcg gataccccgg tgtatgccgc tgtccccggc gtcatcgttc aacctttgta
     3601 cgattggtcg ttcggaatcg ccggtccaca cggaacactc tattatgcgc atatccggcc
     3661 cgttgtccct gtcgggacca cagtaacgcg tgcggccctt gtcgggtata tcgcgaagaa
     3721 tcagggagaa catgtccatc tcgagctccg gccctggcct gccggaatcc tcagagcctt
     3781 tccgggattg tccgagcggg atctcctccg gtcccccctg cgaggtgaag cggttgtact
     3841 gccatcgttt tttcacaata gaacagacaa accgggaaga tgagcaccct ccaggtcagc
     3901 cagtctgtgt ttcactctgg cctgtttcac caaccagtcc ctgctctcag catctcacac
     3961 gatccatact ctctggctct cctcgcaaag gctggactgt ttcataaagc agatcgcata
     4021 tcagaatccc gatcggggcc aaaggcatgg agaaattgca taggcaatat tggcaaaccc
     4081 ggagactggt cagcgccgaa cgcaccaata tttgacggtt ccgtc
//
LOCUS       DAOSHE010000153         4114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19686_length_4114_cov_3.392461, whole genome shotgun sequence.
ACCESSION   DAOSHE010000153 DAOSHE010000000
VERSION     DAOSHE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19686_length_4114_cov_3.392461"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..2074
                     /gene="recD"
                     /locus_tag="PLD82_05340"
     CDS             137..2074
                     /gene="recD"
                     /locus_tag="PLD82_05340"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOG64843.1"
                     /translation="MIQVCDRDEWARSLFEPYLEPSDELPWRVLTAVQEALDSGDAQL
                     ARDLVVLGKPQDPGAALLVVLALLVAGHEGSLCLGLEVTRLTALLTRAGCEPGDAAAA
                     AKRIHAWLKKKNGLGALVTLVGSMEETGTTPMVVLKQDGSFVRVWWQRLFRAERRLEQ
                     AFLERRQLAPVTITASLKKAALARGTAFAGMTLNERQSEAVGMALAARTLVISGGPGT
                     GKTTTVAAVLRALFTAGLCAVGRVALAAPTGRAAQRMKDSLITQTDQMDGLSTEERGF
                     FDALEGKTIHRLLEYHHSRQRFGRCAAYPLEADVVVIDEVSMVDIELMVSLIEAVRTD
                     ARLILIGDRDQLPSVNAGAILADILPAVQRTDGKNPGMAAVILEQNRRSCTEIIEAAR
                     ACNAGDAGSFVNGIPLLEMTQLAEAVRLPLGRIDPGEGVSGKVWKERIQAWLDLVLGG
                     TEAGGYAGMAASSAQQHGADDERIAGEMLARLDSARLLALVRRGRTGVESLNRVAQVW
                     LKERLETLGVSIGRLKPKGEGGAFHGLPIMITANDHERRLFNGDVGVVLGRAAWFREN
                     SGDGMRAWPVLDLGLWEPAFAITVHKSQGSEYGRVLLVFPRETGSRLLVRQIIYTGIT
                     RAKNGVLLWGSEDVIRTVSAG"
     gene            complement(2086..2745)
                     /locus_tag="PLD82_05345"
     CDS             complement(2086..2745)
                     /locus_tag="PLD82_05345"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOG64844.1"
                     /translation="MTRYDPATRCARCHAPLPLCYCHLLPKLACPTFVTLIVHRNEIL
                     RSTATASTTHLCLANSRILPYGRPNEPLDPANLLAPRGMPLYLFPDEDAEVLDADWVA
                     AHPGPWHLVVPDGSWGQAVRMRRRVPILATMRAVCLADPGPSHYQLRTARGPGRLCTL
                     EAVAHALALLEGPAVRDALLQGLAIQVERMKGMRPRFRNQSPRTDAVRTGPDSVVEPE
                     S"
     gene            complement(2753..3181)
                     /locus_tag="PLD82_05350"
     CDS             complement(2753..3181)
                     /locus_tag="PLD82_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64845.1"
                     /translation="MRPLIRPLILAGLLTAGSLHANPVYRLTLSQSPWSAAWQRHSLV
                     FESADALHCEAVLSLDLGIGPQARMPFDWERVHQVRLRGGLDQESGHFQLQGRFQAGQ
                     QTQTILIDAYLLTTHANGRVIVGRYRLGSRAWHGLYGTLE"
     gene            complement(3242..3559)
                     /locus_tag="PLD82_05355"
     CDS             complement(3242..3559)
                     /locus_tag="PLD82_05355"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HOG64846.1"
                     /translation="MQARTSSGRHFPLTKFIAAPILKSMRIPCRFRYPLQSLLISLSM
                     SSVLSLVFTLVHGPAGQDFLPAWLESFAIGIAVGFPVSRLVVPRIIRLCERLSQSSER
                     EDL"
     gene            complement(3638..3943)
                     /locus_tag="PLD82_05360"
     CDS             complement(3638..3943)
                     /locus_tag="PLD82_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOG64847.1"
                     /translation="MLKHIVLWKLKDEATGHTKKENALRIKNLLEDLNGRIDGLIRLE
                     VGFDIGADPAAVDIALYSEFVDRAALDRYQTHPLHEAVKPLVLAVRESRTVVDYEVS"
BASE COUNT          757 a         1187 c         1468 g          702 t
ORIGIN      
        1 tctcccgggg cgggtacgcc gaggtccggg atgggggtgc gccacgggcc gcggatggcg
       61 ccggggtgct ctttttctcc ggtgcggcac tggaggagag ggcagaccgg tacggtatga
      121 acagggaggg cggagcatga tacaggtctg tgacagggat gaatgggcga ggtccctctt
      181 tgaaccgtat ctggaaccgt cggatgagct tccctggcgg gttctgaccg cggtccagga
      241 agcgctcgac tcgggggatg cccagttggc gcgggatctt gtcgtgttgg gaaagccgca
      301 ggacccgggt gcggccctcc ttgtggttct cgccctgctt gtggccggcc atgaggggag
      361 cctctgtctc gggctcgaag tcacgcgcct gactgcgctc ttgacccgtg ccggctgcga
      421 accgggggac gccgcggctg cggccaagcg gatccatgcc tggctcaaaa agaaaaacgg
      481 attgggtgct ctggtgaccc tggtcgggag catggaagag acgggtacga cgcccatggt
      541 cgtcctcaaa caggacggga gttttgtccg ggtctggtgg cagcgtcttt ttcgcgccga
      601 gcgacggctg gagcaggcct tcctcgagcg gcggcaactg gcccccgtca caatcacggc
      661 cagtctcaaa aaagcggccc tggcccgggg gactgccttt gccgggatga cgctcaacga
      721 gcggcagagc gaggctgtcg ggatggcgct tgccgcccgg accctggtca tctcgggagg
      781 tccgggaacc ggcaagacga cgacggtggc cgcggtcctc cgggccctct tcacggccgg
      841 actctgtgcc gttggccgtg tggccctggc cgcgcccacc ggccgggcgg cccagcggat
      901 gaaggactcc ctgatcaccc agacggacca gatggatggc ttgagcacgg aggaacgcgg
      961 atttttcgat gcccttgaag gaaagaccat ccacagactg ctggagtacc accactcacg
     1021 gcaacgcttc ggacgctgcg ccgcctatcc gctggaggcc gatgtcgtgg tcatcgacga
     1081 ggtctcgatg gtagacatcg aactgatggt ctccctgatc gaagccgtcc ggactgacgc
     1141 gagattgatt ctgatcggtg atcgggacca gttgccctcg gtcaatgccg gcgcgattct
     1201 ggcggacatc ctgcccgcgg tgcagcgtac cgacggcaag aaccccggga tggcggcggt
     1261 gatcctcgag cagaacagac gctcctgcac cgaaatcatc gaggccgccc gggcctgcaa
     1321 tgccggtgat gccgggagtt ttgtcaacgg gattcccctt ttggaaatga cgcaactcgc
     1381 cgaggccgtc cggctccccc tgggccggat tgatcccggg gagggtgtct cgggcaaggt
     1441 ctggaaggag cggatccagg cgtggctcga tcttgttctt ggcgggacgg aagcgggcgg
     1501 gtatgccggg atggcggcct cgtcggcgca gcagcacggt gccgatgacg agcggattgc
     1561 cggggagatg ctcgcccggc tggactccgc gagactcttg gcccttgtcc gccgcggccg
     1621 gaccggcgtc gaatcgctca acagagtggc ccaggtctgg ctcaaggaga ggctggagac
     1681 actcggtgtc tccataggcc gcctcaagcc caaaggcgag ggtggggcct tccacggact
     1741 ccccatcatg atcacggcca atgaccacga acgccggctc ttcaacgggg atgtgggcgt
     1801 tgtcctcgga cgggcggcct ggttcaggga gaactcgggt gacgggatgc gggcctggcc
     1861 ggtcctggat cttggtttgt gggaacccgc cttcgcgatt acggtccaca agagtcaggg
     1921 ctccgagtac ggtcgggtcc tcctcgtgtt cccccgggag accggctcgc gacttctggt
     1981 gcggcagatc atctataccg ggatcacccg ggccaaaaac ggggtgctcc tctgggggag
     2041 cgaggacgtc atccggacgg tgtcagccgg gtgatcgaac gcgaatcagg actccggctc
     2101 tacgactgaa tccgggccgg tccggaccgc atcggtccgg ggagactggt tccggaagcg
     2161 ggggcgcatg cccttcatgc gctcgacctg gatggccagg ccttgcagga gcgcatcccg
     2221 gacggccgga ccttcgagca gggccagggc atgggcgacc gcctcaaggg tgcagagccg
     2281 gcccggacca cgggccgtgc ggagttggta gtgcgagggc ccgggatcgg ccaggcaaac
     2341 ggcgcgcatc gtggccagaa tcggcacgcg gcggcgcatg cggaccgcct gcccccagga
     2401 accatcggga acgacgaggt gccagggccc gggatgggca gctacccaat cggcatcgag
     2461 gacctccgca tcctcgtcgg gaaagaggta gagcggcatg ccccggggag ccaaaagatt
     2521 cgccggatcg agcggctcgt tcggcctgcc atagggcaag atccggctgt tggccaggca
     2581 gaggtgcgtc gtcgaagcgg tcgccgtcga gcgcaggatc tcgttacggt ggacgatcag
     2641 ggtgacaaag gtcgggcagg ccagcttcgg caggaggtgg caatagcaga gcggcagggg
     2701 cgcatggcag cgggcacagc gggttgccgg atcatagcgg gtcacgggcc gtttactcca
     2761 gtgtaccgta gagaccgtgc caggcccggc tcccgaggcg gtaacgaccg acgatgaccc
     2821 ggccattggc gtgggtcgtg agcagatagg catcgatcag gatggtctgc gtctgctggc
     2881 cggcctggaa gcgaccctgg agctggaagt gtccgctctc ctggtccaga ccaccccgca
     2941 gccggacctg atgaacccgc tcccagtcaa aaggcattcg ggcctgtgga ccgatcccga
     3001 gatcaagaga caggaccgcc tcgcaatgta aagcatcggc ggactcaaag acaaggctat
     3061 ggcgctgcca ggcagccgac cacggggatt gggagagtgt cagacggtag accgggttgg
     3121 catggagact gcctgctgtc aacagaccgg cgagaatcag gggacggatc agggggcgca
     3181 tgcttccttc cagtgcggaa caagatttct gcccgcgcct gaaatatagc acgccggacc
     3241 gtcagaggtc ttccctctcg gaagactgac tgagccgctc gcacaagcgg atgatgcgcg
     3301 ggacaaccag gcgcgaaacg ggaaagccga cagcgatccc gatggcaaag gattcgagcc
     3361 aggccgggag aaagtcctgc cccgctggtc catggaccag cgtgaagacc agtgagagaa
     3421 cggaggacat ggagagcgag atgagcaggc tctgcagcgg atagcggaag cggcagggga
     3481 ttctcatgga cttgagtatg ggggctgcga tgaattttgt caaggggaaa tggcgtccgc
     3541 tactggtccg tgcctgcatc gctggtccgg cggatgcaca cgtctttgtg gcttggctgg
     3601 atgcgaatcc aatgagaggg tacaagcccg ggtgtgttca gctgacctcg tagtcaacga
     3661 cggtacgcga ttcgcggacg gccagcacga gcggcttgac ggcttcgtgc aaggggtgtg
     3721 tctgatacct gtcgagggca gcccggtcaa cgaactcgct gtagagggca atatcaactg
     3781 cagccggatc cgcaccgatg tcaaacccga cctcgagacg gatcaatccg tcgatccgtc
     3841 cgttcaggtc ttccagcagg tttttgatgc ggagggcgtt ttcctttttt gtgtgtcctg
     3901 tcgcttcatc cttgagtttc cagagtacga tatgcttgag catgggatca cctgtcaggg
     3961 ggggggtggc gcagcagggc tgcggtatga gcatcatgcc ctaaaggcgt aacgatgtca
     4021 agcacaacgc gcacgcgcag cgggcaacgc ggcgcgaaag gggtgggatg ggcgagcgtg
     4081 ttattcaatc cgatgatgga gggacgtgat ggtt
//
LOCUS       DAOSHE010000154         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19720_length_4110_cov_3.619975, whole genome shotgun sequence.
ACCESSION   DAOSHE010000154 DAOSHE010000000
VERSION     DAOSHE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19720_length_4110_cov_3.619975"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..797
                     /locus_tag="PLD82_05365"
     CDS             84..797
                     /locus_tag="PLD82_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64848.1"
                     /translation="MRRKDFVRKASKTFPVARERMHLLREPQPDSNSLFVAGTKHPVS
                     EVLYGEFEYLQDFEYLDRATQDRGSAILAVFGRERSEKENLACHDKLKNLLCERGLRY
                     HEVYGMYQQVPCDAEAHDFDSGLVHEDLLLCHVPCNPDSVSDFESFSAWMLEIVTHKL
                     ETAALLLVAPYTRSAMYREHETSINFDMARCDVQASLELFIKSRMQGPEETAIVSRWA
                     IRKPASFNDALLMQKKGIL"
     gene            794..1273
                     /locus_tag="PLD82_05370"
     CDS             794..1273
                     /locus_tag="PLD82_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64849.1"
                     /translation="MNTGEIYRGVGIRLSQDLVDGFCRRSADPTHDERSWRETILAMT
                     RETLDALIDLDNESEKILAYSTSMCFGLVLYNDFKAKTINLATILPVRKDYPVRTAED
                     VFRMIEQDCLATVDPSEHRELEQEGMCHIVRLYDASLIAVLYEGKYYDGDYVVIVVG"
     gene            1497..1994
                     /locus_tag="PLD82_05375"
     CDS             1497..1994
                     /locus_tag="PLD82_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64850.1"
                     /translation="MMKVKNLFVGISLVCIASTPAFSDGVLPPGFDMQRFMQSRAYAV
                     HGEYLRDTETRRMMPVLIQTMSAVAKRCGREPNLLSRICFLAQSIDETMRCHGVELGC
                     VQAYWQGLDSVTNPNATWKPDLGQRDILIRYWKNHEAFFQMVYINRFKEHFVYDFAAD
                     FLSLK"
     gene            2096..2479
                     /locus_tag="PLD82_05380"
     CDS             2096..2479
                     /locus_tag="PLD82_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64851.1"
                     /translation="MKIVALAGMMFVLAGCHLIFGDDGGSEIRQKVLELAQKHGAQQL
                     DIPAGTFKALELQNIKSIDVGRLPHGQRYFLFGEGMGNDWKACNAYLKAEDRGEIGTV
                     PLVYVWKHNKETDMYLVKVYLGIRM"
     gene            2497..3144
                     /locus_tag="PLD82_05385"
     CDS             2497..3144
                     /locus_tag="PLD82_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64852.1"
                     /translation="MTEQISMTNEQHERKYAFFKNRSFAEKMCDSRVLVNRKRFFDKK
                     PLLEHVDETECVGLVTILDNLEERGMLADWAIGGATALSNYTIAIPTVGIDIFGHFPM
                     ADGFQAVAAWLKQTYDAVEYDAMLKIGSLYLRFFPLESSTPVDREAAAKAVRIAGGFV
                     IFPVEYLICSMLLANQKRQYPYLLLMKSEQPYDPATLQAVLEKFALRERWDALPV"
     gene            3352..>4110
                     /locus_tag="PLD82_05390"
     CDS             3352..>4110
                     /locus_tag="PLD82_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG64853.1"
                     /translation="MLQESLKEFVVYASNLKGDEKGEAQLFCDRFFRAFGHGGLIEAN
                     GTLEARIKFQSGKTKFADCLWSPPDRPGVLIEMKKKSETNLEKHFPQARDYWIEMNPE
                     VVIGSGAQKPEYIILCNFEKFLIYRQLSLVDEVLLSDLLDRSSAFNFMLPVIKEPIFK
                     NNVEAISQEVAGTIGELYKYLVYEKRENTEAAQHFLLQCVLALFSEDFGLIPNNFFSE
                     LIRDCQNGESSYDLFGALFKQMASEAPAKAGKFKD"
BASE COUNT         1102 a          871 c         1097 g         1040 t
ORIGIN      
        1 tttgccagct ttcaggaaat cgatattgcc tgatttgtgt cgcaacaggc aggaatgctc
       61 tacgtcatat aaggaggaag tggatgcgca ggaaggattt tgtcagaaaa gcttcaaaga
      121 cctttccggt tgccagagag cggatgcacc ttctgaggga gccccagcct gattcgaaca
      181 gcctgttcgt cgctgggaca aaacatcctg tttcagaagt cctgtatgga gagtttgaat
      241 atctgcagga tttcgaatat cttgaccggg caacacagga caggggaagc gcgattcttg
      301 cggtctttgg acgggaacga tccgaaaaag aaaacctcgc ctgtcacgat aaattgaaaa
      361 acctgctctg cgaacggggg ctccggtacc atgaagtgta cgggatgtac cagcaagttc
      421 cgtgcgatgc cgaggcgcac gatttcgatt ccgggcttgt gcatgaagat cttttgctct
      481 gccatgtccc gtgcaacccg gacagtgtgt cagactttga atcattttcc gcctggatgc
      541 tggagattgt gacgcacaag ctggaaacgg ctgccctcct gctggtcgcc ccctatacac
      601 gatcggccat gtaccgggaa cacgaaacca gcatcaattt cgatatggcc cgctgtgatg
      661 tccaggcgag tcttgaactc tttatcaagt cacggatgca ggggcccgag gaaacagcca
      721 tcgtatcccg ttgggccatc cgcaaaccgg cctccttcaa tgacgccctg ctgatgcaga
      781 aaaaggggat cttatgaata ctggcgagat ctatcgaggg gtcgggatca ggctgagtca
      841 ggatctcgtt gacgggttct gtcgtcgcag tgctgacccg acgcatgatg agaggtcatg
      901 gcgtgagacg atcctggcaa tgacgagaga gactctcgat gccctgattg atttggacaa
      961 tgaatccgaa aaaatcctgg catactcgac aagtatgtgt tttgggcttg tgttgtataa
     1021 tgattttaaa gccaaaacga tcaatcttgc aaccatcctg ccggtcagga aagattatcc
     1081 agtgcggact gccgaggatg tgtttcgcat gatcgaacag gactgtcttg ccacggttga
     1141 cccttcggaa cacagggagc ttgaacagga agggatgtgc catattgttc gcttgtatga
     1201 cgcatccctg attgctgttc tatatgaagg gaaatactat gacggtgatt atgttgtaat
     1261 cgtcgtcggg taaaaaacat cccgtaataa aaaatcaaaa aaacagtgaa catttgaacg
     1321 ataaaccgcc tatatatcat gacggcgcaa catgcctgaa aatcatatag gcggacactg
     1381 tcaaaacgtc cgaatcgggc gtctctataa aaaccgatac aattcgtatg aattccggat
     1441 gcgtataagg gcgacgtctg ggcatttaag aatattacaa gaagaaaaga ggaaacatga
     1501 tgaaagtcaa aaatttgttt gtcgggatca gtctggtttg cattgcttcg acaccggcat
     1561 tttcggatgg ggttttgcca cccggatttg acatgcagcg gttcatgcag agcagggcct
     1621 atgccgtcca tggagagtat cttcgcgata ccgagacacg ccgcatgatg ccggtcttga
     1681 tccagaccat gagtgcggtt gccaaacgat gcggtcgcga gccgaatctg ctctcgagaa
     1741 tctgttttct tgcccagagt attgatgaaa cgatgcgatg ccatggtgtc gagcttggct
     1801 gtgtccaggc atactggcag ggtctcgatt ccgtcacgaa tccgaatgca acctggaaac
     1861 ccgatcttgg ccagcgggat atcctgatcc ggtattggaa aaaccacgaa gccttcttcc
     1921 agatggtcta catcaaccgc ttcaaggagc actttgtgta tgactttgcc gccgattttc
     1981 tctcgctcaa gtgaggttga aaggcgtgga aagaggttga ggagtgtttc cccctgcgac
     2041 ctcggaaatc cgggttgcag gattgaataa aaaacaaata cagcaggagg aagtcatgaa
     2101 aatagtcgca cttgcaggaa tgatgtttgt attggccgga tgccatctga ttttcggaga
     2161 tgacgggggt tcggaaattc ggcagaaagt gctggaactc gcacaaaaac atggagcgca
     2221 gcagttggat ataccagccg ggacattcaa ggcattggag ctgcagaata tcaaatccat
     2281 cgatgtgggc aggctgccac atgggcaacg ctactttttg tttggagagg gcatgggaaa
     2341 cgactggaag gcctgtaatg catatctcaa ggctgaagac cggggagaga tcgggactgt
     2401 ccccttggtc tatgtatgga agcataataa agaaacggat atgtaccttg tcaaggtcta
     2461 tcttggaatc agaatgtgac ggaagcgtag cgcacagtga cggaacaaat atccatgacc
     2521 aatgagcagc acgaaaggaa atatgcattc ttcaaaaaca gatcctttgc cgaaaagatg
     2581 tgcgactcgc gggtactggt aaacaggaag cggttttttg acaaaaagcc gttgcttgag
     2641 cacgtcgatg aaaccgagtg tgtcgggctt gtcacgattc tggacaatct tgaggagagg
     2701 gggatgctgg cggattgggc gatcggcggg gccacggcct tgtccaatta caccatcgcc
     2761 atcccgacag tcggcatcga catcttcggg cactttccga tggcagacgg attccaggct
     2821 gttgctgcct ggctgaaaca gacatacgat gcggttgagt atgatgccat gttgaaaatc
     2881 ggcagcctct atctgcgttt tttcccgctt gagtcgtcca ctcccgtgga tcgtgaggcc
     2941 gcagccaaag cagtccgtat cgctggcggc ttcgtgatct ttccggtcga gtatctgatt
     3001 tgctcaatgc tgctggcaaa ccagaaacga cagtatccct acctgttgtt gatgaagtcc
     3061 gaacaaccct atgatcccgc cacgcttcag gctgtactgg aaaaatttgc ccttcgcgag
     3121 agatgggatg ccctgcctgt gtagggatgt ggggccgatg atggtaatga cttgaaaatg
     3181 ctttagaaat tgagtgaaga attgactttt gattggatgt gcaggataat gattcggcct
     3241 gatcggttgc atttgacggg atggtttgtg tgcttctaat ttgttttgtt ggcattcgga
     3301 gaaggatcga cttttgccgt attatcatga attacaacac aggagtagtc aatgctacag
     3361 gagagtttga aagaatttgt agtgtatgcc tcgaatctaa aaggcgatga aaaaggcgaa
     3421 gcgcaattgt tttgcgatag atttttcaga gcttttggcc atggaggctt aatcgaggcc
     3481 aatgggactc ttgaagcccg aataaaattt caatccggca aaacaaaatt tgcagactgc
     3541 ttgtggtcgc caccggatcg gccgggtgtt ttaattgaga tgaagaagaa atccgagaca
     3601 aatcttgaaa aacattttcc acaggcaaga gactactgga tagagatgaa tccagaagtc
     3661 gttattggaa gtggtgctca gaaaccggaa tatataatct tgtgtaattt tgaaaaattt
     3721 ttaatctatc gtcaactatc tttggttgat gaagtactgt tgagtgattt gttggacaga
     3781 tcaagcgctt tcaatttcat gctacctgtc attaaagaac cgattttcaa gaataatgtc
     3841 gaggctatat cacaggaagt agctggaaca atcggagagc tttataaata tctggtttat
     3901 gaaaaacgag aaaacacgga agcagcacaa cattttttat tacaatgtgt tttagccctt
     3961 ttttctgaag actttgggct tataccgaat aatttttttt cggagttgat ccgggattgt
     4021 caaaacggtg aaagctcata tgatttgttt ggcgcacttt ttaaacaaat ggcttcagag
     4081 gcgcctgcaa aagcgggaaa attcaaagac
//
LOCUS       DAOSHE010000155         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19806_length_4096_cov_3.841376, whole genome shotgun sequence.
ACCESSION   DAOSHE010000155 DAOSHE010000000
VERSION     DAOSHE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19806_length_4096_cov_3.841376"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLD82_05395"
     CDS             complement(<1..657)
                     /locus_tag="PLD82_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG64854.1"
                     /translation="MDRLQEIARRRTFAIVSHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKRAATSDFMEIEKQRGISVATAVMTFEYDDIIINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVVDSVKGVEEQTEKLMNVCRMRATPVTIFVNKMDREGKDPFDILDELEYKLD
                     IKVSPLSWPINKGSTFKGVYNIFRGSLDLFRPNKTKIEEEVISISSLDDSLLEEYLYA
                     D"
     gene            complement(824..1531)
                     /locus_tag="PLD82_05400"
     CDS             complement(824..1531)
                     /locus_tag="PLD82_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG64855.1"
                     /translation="MEHNIFEAAMHGNIAALERCLQSGADISAASREREAWTPLVLAA
                     GNGHLEMVKCILAKGAGVNQTIPRGEYAGWTGLMRASWGGHPEVARFLIEQGADVNLA
                     NEYGGTALMHASIEGHPSVVRLLLANGAVVDAEDNNGLTAMMLAAIAGTEETVRILLE
                     HGAAVDHRAGSMAGGGTALMLAAHEKHARIVHILLDYGADAHVRNAYGLDALTIAQRA
                     GSQTIIRMLEKAGRRQV"
     gene            1680..2858
                     /locus_tag="PLD82_05405"
     CDS             1680..2858
                     /locus_tag="PLD82_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64856.1"
                     /translation="MKIVRRRIARSRIRKAGLNPLEACLLSRLAESSADLTTLTALAA
                     EKDFENSLVSISLREHTASALARLEQAGLVLDDGTHYALTQKGHQLVAKLANNDAFHD
                     VPSPVLASHLTVILYFSAAGLSILGFILTGSAELIGWGSIILAWIGSFLLAWHGIRRK
                     KERTAARIIGFGILFLSAGLLFLGVFSLFSPFIVRRLLWITITLAIPGITALALLVLA
                     QMHMQIARKHASFSFAALAHAARRHALLSMAAILACMASYVSLHFVVSVFLILTGVRL
                     LFSAARMLEALRKRRTASAALVHWFEHMARHSREHFFLAWLEQIISERGYTKSEILGI
                     YRQNFVSQTTPFFDEINVNLARDAWFEEHVDVLLGHLLFDDRARIEDDRFIHASRRPM
                     "
     gene            2910..3629
                     /locus_tag="PLD82_05410"
     CDS             2910..3629
                     /locus_tag="PLD82_05410"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOG64857.1"
                     /translation="MNTVWSINEQRPRERLFRLGPGALPDLELLTAIIGSGTANDPAC
                     AIAARLLETVNGSLLGLFRTHPGELVNVPGIGSARCAAILGGMELGKRMLLRSEQKGN
                     CIISPNDAWAVFRERMPLDSCESILALYLDNRSHVLLCDEVAAKRRPAGCDLDMRRLV
                     RSSLMVNAAGVILAHNHPSGECTPSEQDVYLSLQVEAMLGCIGVELIDHLVTGQERFT
                     RVEWRGRTPPPVMQILRNRQA"
     gene            complement(3626..>4096)
                     /locus_tag="PLD82_05415"
     CDS             complement(3626..>4096)
                     /locus_tag="PLD82_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG64858.1"
                     /translation="RVLIVVDGSAAGVRAAKFSIMVARRTGAELFAISVVDTATIREL
                     ELSRIFVEEEGREYERSLEEDAKKHLHYIASLAESKKCHVETVLERGSVYGRIVEFAM
                     EKEIDLIVIGGEIGQDRGGARDRASLEYLRVLREARQSVLFVKNPDVDVDFKKL"
BASE COUNT          836 a         1340 c         1006 g          914 t
ORIGIN      
        1 atcagcataa agatattctt ccaaaagact gtcatcaagc gatgaaattg aaattacctc
       61 ttcttcaatc ttagttttgt tcggacgaaa taaatccagg ctgcctctaa aaatattata
      121 aacaccttta aatgtacttc ctttattaat cggccagctt agaggtgata cttttatatc
      181 gagtttgtat tccagttcat caagaatatc gaaaggatct ttcccttccc ggtccatttt
      241 atttacaaaa atagtaaccg gtgtggcgcg catacggcaa acattcatca gtttttcagt
      301 ctgttcctct acacctttca cagaatcaac aacaagaatt acactgtcaa ctgcagtaag
      361 tgtgcggtag gtatcctcgg caaaatcttt gtgtccggga gtatcgagca aattgataat
      421 aatatcatca tattcaaatg tcattacagc agttgctacc gaaatacctc tctgcttttc
      481 gatctccata aagtcggacg tcgcagcccg cttgatcttg ttggacttga cagcaccggc
      541 gacctggatc gcgcctccaa acaggaggag cttttcggtc aacgtggtct tccccgcgtc
      601 cgggtgactg acaatggcaa aggtcctccg gcgtgcaatc tcctgcagtc tgtccatccg
      661 cttcctccac aaatacccgg gccggaaaat tcccgctggc acccggcact ttgaggaaaa
      721 aatctacagg caggacaggc gggaaacccg tttttttttc aggttcatga ggaaaggcca
      781 ttgcgcactg cccgacacgc cagctctttc gccggaacat ctctcagacc tggcgccgac
      841 cggccttttc aagcatcctg atgatggtct ggctcccggc ccgctgcgca atcgtcagcg
      901 catccagtcc gtaggcattc cgcacatggg catccgctcc gtaatcgagc aggatgtgta
      961 caatccgggc atgtttttcg tgagcggcca gcatcaacgc ggtcccgcct ccggccatgc
     1021 tccccgcgcg atggtcaaca gcggccccgt gttccagcag gatccggacc gtctcctcgg
     1081 ttccggcgat ggctgccagc atcatcgcgg tcaacccgtt attgtcctcc gcatcgacca
     1141 cagccccgtt ggccagtagc agccggacaa ccgagggatg cccctcgatc gaggcatgca
     1201 tcagggctgt tccgccgtat tcattggcca gattgacatc cgcaccctgt tcgatcaaaa
     1261 accgggccac ctcgggatgg cctccccagg atgcccgcat caaaccggtc cagcccgcat
     1321 actccccacg ggggatggtc tgattgacac ccgcaccctt tgccaggatg cacttgacca
     1381 tctccagatg cccgttcccc gccgccagga caaggggggt ccaggcctcc cgctcacggc
     1441 tggcagcact gatgtcggct ccggactgaa gacaacgctc cagcgctgca atattcccgt
     1501 gcatggccgc ttcgaagata ttgtgctcca tcccgccttc ctccccgttc cgtctgcgcg
     1561 cagtataccc gcccgggcaa gggaatacaa gcatactgtc aggacacagt gtttttccgg
     1621 cccggcttcc cgacagagcg gccttgcctg tctccggcac aaatctgtat ttttgtacca
     1681 tgaaaatcgt acgccgccgg attgcccgct cccgtatccg caaagccggg ctgaatcctc
     1741 ttgaagcctg tctcctcagc cgtctggccg agtcatccgc cgatctgaca accctgacgg
     1801 cgctggcggc cgaaaaagat ttcgaaaaca gccttgtttc catcagcctg cgcgaacaca
     1861 ccgccagtgc tctggcacga ctcgaacaag ctggcctggt tctggatgac ggcacacact
     1921 atgccctcac gcagaagggg caccagcttg tggcaaagct tgccaacaat gacgccttcc
     1981 acgacgtgcc ctccccggtc ctcgcatccc acctgacggt catcctctat ttctctgctg
     2041 ccgggctctc catcctgggt ttcatcctga ccgggagtgc cgaactcatc ggctggggga
     2101 gcatcatcct ggcatggatc gggtcctttc tcctcgcctg gcacggaatc cgccggaaga
     2161 aggaacggac tgccgcccgc atcatcgggt tcggcatcct ctttctgtcg gcagggctcc
     2221 tcttcctcgg agtcttcagt ctcttctctc ccttcatcgt ccgccggctc ctctggatca
     2281 cgatcaccct ggccattccc ggcatcaccg cgcttgccct tctcgtcctg gcacagatgc
     2341 acatgcagat tgcgcgaaaa catgcctcgt tctccttcgc ggcactggca catgccgccc
     2401 ggcgacatgc cctcctctcg atggcagcca ttctggcctg catggcatcc tacgtctcac
     2461 tgcattttgt cgtctcggtt ttcctgatcc tgacaggggt acgacttctt ttttcggcag
     2521 cccgcatgct cgaagccctg cgcaaacgac gcacggcttc agccgccctc gtccactggt
     2581 tcgaacacat ggcgagacac agccgggaac acttctttct ggcctggctc gagcagatca
     2641 tcagtgaacg cggctatacc aagtcggaga tcctcggcat ctaccgccag aacttcgtct
     2701 cccagacaac gccctttttt gacgagatca atgtcaacct cgcccgggac gcctggtttg
     2761 aggaacacgt cgatgtcctc ctcgggcacc tgctctttga cgaccgggcg agaatcgagg
     2821 acgaccgctt catccacgca tcacggcgac cgatgtaatt tctttgaaaa aagtgggcac
     2881 cgtcaccgtc tgccggactg tactcaggta tgaacacagt ctggtccatc aatgaacaac
     2941 gtccccgtga gcggctcttc cgccttggcc cgggagcact ccccgatctt gaactcctga
     3001 ccgccatcat cggaagcggc acggccaatg atcccgcctg tgccatcgcg gcacgcctgc
     3061 tcgaaactgt caacggttcc ctcctcgggc tcttccgcac acaccccggg gagctggtca
     3121 atgtcccggg tatcgggagt gcccgctgtg ccgccattct tggcgggatg gaactgggaa
     3181 aacgcatgct cctccggtcc gaacaaaaag ggaactgcat catctcgccc aacgatgcct
     3241 gggccgtctt ccgcgaacgc atgccccttg acagttgcga gtcaatcctc gctctctacc
     3301 tcgacaatcg cagtcacgtc cttctctgtg atgaagtggc agccaaacgc cgcccggccg
     3361 gatgcgatct tgacatgaga cggcttgtcc gttccagcct gatggtcaat gccgccggtg
     3421 tcattctcgc acacaaccat ccatccggtg aatgtacccc gagcgagcag gacgtctatc
     3481 tctccctcca ggtcgaagcg atgctgggct gcatcggtgt ggaactgatc gaccaccttg
     3541 tcaccggcca ggaacggttt accagagtcg aatggcgggg acggacgcca cctccggtca
     3601 tgcagatcct gcgaaaccgc caggcctaga gcttcttgaa atccacgtcg acatcgggat
     3661 tcttcacaaa caggaccgac tgccgggctt cccgcaagac ccggagatac tcgagggatg
     3721 cccggtcacg cgcccccccc cggtcctggc cgatctctcc cccgatcacg atcaggtcga
     3781 tctctttttc catggcaaac tcgacgatcc ggccatacac cgaaccacgt tcaaggacgg
     3841 tttcgacgtg acattttttc gattccgcga ggctggcaat ataatgcagg tgctttttgg
     3901 catcctcttc caggctgcgt tcgtattcac ggccctcttc ctccacaaag attcttgaaa
     3961 gctcgagctc acggatggtt gccgtatcca ccaccgagat ggcaaagagt tccgcccccg
     4021 ttctgcgggc aaccatgatg gagaatttgg ccgccctgac tccggctgca gatccgtcga
     4081 caacaatcag aacccg
//
LOCUS       DAOSHE010000156         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19807_length_4096_cov_3.331849, whole genome shotgun sequence.
ACCESSION   DAOSHE010000156 DAOSHE010000000
VERSION     DAOSHE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_4096_cov_3.331849"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLD82_05420"
     CDS             complement(<1..996)
                     /locus_tag="PLD82_05420"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOG64859.1"
                     /translation="MSIQERYRPEKKRTPLTMTLRLVLSALIISAIIRLFFFETVTID
                     GPEMLPGVSRGESILVNRFATGFRVPLFNSLRIFGFYTPEVGDLVLIKHPWQGWSGIA
                     EFFDICTASLFGLTGDGKMRLVRVMARGGQHIRIDASGTVHVDGKVIQKKRIKGNLTL
                     RNRADKNGNVTRSVILNRMVLDQSIVQGDPLPDLTLSLYAEGSWKTVESEDGSGALPA
                     RIFPVPVTNDALAMTWATRWLALTVLREENAKIAVLKAGQKGQSPAVFRDDTGNLWYQ
                     LENEDPKKLVDVQSGEAWLIVPEDMLFVLNDLRDAMYDSRIWGPVFEDSITGIPML"
     gene            1272..2210
                     /locus_tag="PLD82_05425"
     CDS             1272..2210
                     /locus_tag="PLD82_05425"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOG64860.1"
                     /translation="MTNVDIEKLVETSDEWIRERTGIRQRHICAPGESASDLAVAACK
                     RMQAEFPAAFEGIDFILVATTTPDHPLFPSVASLVGNRLGLGFVPCVDLSAACTGFVY
                     SLSAATAWIRSGMYKKILLVCVDTLSHFLDWTDRSTCILFGDGAGAMILEAGPEDSPS
                     DALGFELHADGGGSNMLIVPEGGSRKPFSAETLANRGQFLRMDGPAVYKFAINAITES
                     VNAVLEKSSLRVSDITHFIPHQANIRIIDSAAKKIGLAPDVIRTNLDRCGNTSSASIP
                     IVLDELNRQGKLTRGDILVTVGFGAGLTWGASVLRW"
     gene            complement(2193..2597)
                     /locus_tag="PLD82_05430"
     CDS             complement(2193..2597)
                     /locus_tag="PLD82_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64861.1"
                     /translation="MKFVLIATLLVAGCSPSREVRIQQDGQKAGIVLAMGPRVMRNGV
                     FFAGEFDEGTKSVTVAGSFNAWNPVQFFLTNTLGTRVWSGYVPMSTPGEIHFCYVIDG
                     RWRKPDPLTEKKEDDEGFEYSVFPGLSLPAKN"
     gene            complement(2594..3055)
                     /locus_tag="PLD82_05435"
     CDS             complement(2594..3055)
                     /locus_tag="PLD82_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG64862.1"
                     /translation="MKSFSVVLLFALTVGAWVLFAEDRQEPKIRIGMGNSQLTVEVAA
                     TPGERARGLMYRDSLDDDTGMLFVFEYPGRWGFWMKNTLIPLSIAFLDHEGNVVNIED
                     MQPHDTTVTMPKGEILYALEVNLGWFARHGIRPGSRIALPEQVTKIVGNQR"
     gene            complement(3052..3948)
                     /gene="lpxC"
                     /locus_tag="PLD82_05440"
     CDS             complement(3052..3948)
                     /gene="lpxC"
                     /locus_tag="PLD82_05440"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG64863.1"
                     /translation="MKQKTVSGQIVLQGKGLHSGQDVAVRITPAEPGSGHVFFREDLA
                     PGMPIPASWRFVLDTGHNVTIGRESVSIQTVEHLLSSLSATGIDNCHIHVHGPEIPLC
                     DGGAKTFFEAINQTGLQEQEADREYCRIAIPVWVEDGDRYLLALPANDLRISCSIHFK
                     HKSIRYQACHIRMDTATFADEIAPARTFGFTGDIEMLRKQGLARGGDYESVLVFDEAG
                     PISPDLHFEDECVRHKVLDIMGDLALLGRPLRAHIIGHKTGHALDVEFVRKLAREMIP
                     VRDTDALERDREFVNFCERMNL"
BASE COUNT          977 a         1214 c          950 g          955 t
ORIGIN      
        1 cagcatggga atcccggtaa tggagtcttc aaaaaccggg ccccagatcc ggctgtcata
       61 catcgcatcc cgcagatcgt tgaggacaaa caacatgtcc tccggaacga tgagccaggc
      121 ttcaccggat tggacatcaa ccaatttttt cgggtcttcg ttttccaact gataccagag
      181 atttcctgtg tcgtcccgga aaacagccgg cgattgtcct ttctgacctg ccttcaacac
      241 agcaatcttg gcattctctt cacgaaggac ggtcaaggct agccagcgtg tggcccaagt
      301 catggccaga gcatcgttgg tgaccggtac gggaaagatc cgcgcaggaa gagcaccgct
      361 cccgtcctcg ctttccaccg ttttccagct tccttcagca tagagtgaaa gtgtcaagtc
      421 ggggagaggg tctccctgaa caatgctttg gtccaacacc atccggttga ggatgacgct
      481 ccgtgtcaca ttcccgtttt tatcagcccg atttctcaag gtcaggtttc ccttgatccg
      541 ctttttctgg ataacctttc cgtctacatg caccgtcccg gaagcatcaa tccggatgtg
      601 ttgtcccccc ctggccatca cccgcacaag ccgcatcttc ccgtctccgg tcaagccgaa
      661 gagcgatgcg gtacagatat caaaaaactc ggcgatacca ctccacccct gccacggatg
      721 cttgatcagg acaaggtctc caacctccgg agtgtagaaa ccgaaaatcc tgagcgaatt
      781 gaacaacggc accctgaatc cggttgcgaa cctgttgacg agaatgctct ctccccggga
      841 aacaccagga agcatctcgg gtccatcaat cgtcacggtt tcaaaaaaaa agagccggat
      901 gatggctgaa atgatcagtg ctgacaggac gagccgcagg gtcatggtca atggcgttcg
      961 tttcttttcc ggtctgtatc gttcctggat gctcattact gtactctccc aacctcatct
     1021 tgaattccac acaacagagg cactgacaaa aacactcctg aaaacgctcg tgtcgacaac
     1081 aagctgtccc gccaaaaccc tcataaaaga tatactcttc catgattcaa gataacgtct
     1141 tgtttttggc gcaaactgcg ctacatttat tgataacaca aatccgccat agcctgcggt
     1201 gatgacgcaa ggagtctgcc atccaaaccg tacgcatatc cgccaccgga agcgctctcc
     1261 cctctcgagt cttgaccaat gttgatatcg agaaacttgt cgagacctcc gacgaatgga
     1321 tccgggaacg aaccggcatc cgccaacgcc atatctgcgc acccggtgaa tccgcttcgg
     1381 atcttgctgt cgccgcctgc aagcggatgc aagccgaatt ccccgcggct tttgaaggaa
     1441 ttgacttcat tctcgtcgca acaacaacac ccgatcaccc gctcttcccg tccgttgcat
     1501 ctcttgtcgg gaaccgtctg ggactgggat ttgtcccttg cgtcgatctt tcggccgcat
     1561 gcacgggctt tgtctattct ctctctgccg cgaccgcctg gatacggagt ggcatgtaca
     1621 aaaaaatcct tctggtctgt gtggatacac tgtcgcattt tctcgactgg accgatcgat
     1681 cgacctgcat tctcttcggt gatggtgccg gtgccatgat tcttgaagct ggtcctgaag
     1741 acagcccgag cgatgcgctg ggatttgaat tgcacgcgga cggaggtggc tcaaacatgt
     1801 tgatcgtccc ggagggcggc tcccgtaagc ctttttcggc agagacactg gccaatcgcg
     1861 gccagttctt gcggatggac ggccctgccg tctataaatt cgccatcaac gccatcaccg
     1921 aatcggtgaa tgccgtcctt gagaaaagca gtctgagagt ttcggatatc acccacttta
     1981 taccgcacca ggcgaatatc cgcatcatcg attctgccgc aaaaaaaatc gggctcgccc
     2041 ccgatgtcat ccggacaaac ctcgaccgat gcggaaacac atcatcggcg tccatcccga
     2101 ttgtactcga cgaactcaat cgccagggga aactcacccg gggcgatatc ctggtaaccg
     2161 tcggctttgg tgccggattg acgtggggag cttcagttct tcgctggtag ggacagtccc
     2221 gggaagaccg agtactcaaa gccttcgtca tcttcttttt tttctgtcaa ggggtccggt
     2281 tttcgccatc ttccatcaat gacgtagcaa aagtggattt ctccgggagt actcatcgga
     2341 acataaccgc tccaaaccct ggttccgagt gtgtttgtca aaaaaaactg taccggattc
     2401 catgcgttaa aggaacccgc taccgtcaca ctttttgttc cttcgtcaaa ctcgccagca
     2461 aaaaaaacac cattccgcat cactctggga cccatagcga gcacgatccc ggccttctgt
     2521 ccgtcttgtt gtatacggac ctctctcgaa ggagaacacc cggccacaag aagagtggca
     2581 atcaggacaa acttcatctt tggttaccga cgatctttgt gacctgctcg ggaagcgcta
     2641 tccggctgcc gggccgtatc ccatgccttg caaaccagcc caggttgact tcgagcgcat
     2701 agagtatctc cccttttggc atggtcactg tcgtatcatg gggttgcata tcttcaatat
     2761 ttaccacatt tccttcatga tcaagaaacg cgatagaaag cggaatcaac gtattcttca
     2821 tccaaaaacc ccaccgtccc ggatactcga agacaaacag catgcctgtg tcgtcatcaa
     2881 gggagtcccg atacatcaag ccacgagccc gttccccggg tgttgccgcc acctcgacgg
     2941 tcaactgcga atttcccata ccaatccgga tttttggctc ttgcctgtct tcggcaaaaa
     3001 ggacccacgc gccaactgtc agcgcgaaca gcaacaccac actgaaactc ttcaaagatt
     3061 catcctctca cagaaattga caaattcacg gtcccgctca agcgcatctg tatcccggac
     3121 cggaatcatc tcccgggcaa gtttgcggac aaattccaca tcaagggcat gaccggtctt
     3181 gtgcccaata atatgggcac gcagtggacg accaagcaat gcgagatcac ccatgatatc
     3241 caataccttg tgacggacac attcatcctc aaaatgcaga tccggactga tcggaccggc
     3301 ttcatcaaac acaagcacac tctcatagtc tccgcctcgg gccagcccct gtttgcggag
     3361 catttcgata tcaccagtaa aaccgaaggt tcttgccggt gctatctcgt ctgcaaacgt
     3421 ggcagtatcc attctgatat ggcaagcctg ataccggatc gatttgtgct tgaaatggat
     3481 ggagcaggat atgcgcaaat cgtttgccgg gagtgccagg aggtagcggt caccatcttc
     3541 aacccagacc ggtatggcta tccggcaata ctctcggtca gcctcttgct cctgcaatcc
     3601 agtctgattg atggcctcaa aaaatgtttt ggcgccacca tcacacagcg gtatttcggg
     3661 accatgcaca tgaatatggc aattatcgat acctgtcgcc gaaagggagg acaaaagatg
     3721 ctccaccgtc tggatggaaa cgctctccct gcctattgtg acattgtgcc ccgtatccag
     3781 cacaaaacgc caggaagccg gtatgggcat accaggcgcc agatcctccc gaaaaaaaac
     3841 atgcccgctc ccgggttcag ccggcgtaat gcgcactgca acatcctgtc cgctgtgcag
     3901 acccttgcct tgaagcacta tctgccctga aaccgttttc tgcttcatcg ttctccaccg
     3961 gaatccttca agcctatcca gaaatgtatc acaattgcct atagatatcc gctgcatcaa
     4021 gataccgttg caaaatcacg gccgctgccc aatcgtccat gaattgaccg ggtttttttt
     4081 tcctcccggt ggcatg
//
LOCUS       DAOSHE010000157         4082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_19902_length_4082_cov_2.734045, whole genome shotgun sequence.
ACCESSION   DAOSHE010000157 DAOSHE010000000
VERSION     DAOSHE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19902_length_4082_cov_2.734045"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLD82_05445"
     CDS             <1..478
                     /locus_tag="PLD82_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG64864.1"
                     /translation="HMIPQSKFAEAAPMVSDAHHVTATDGKVNGMRSNYPHGNVASAT
                     WTSKNGGKLGAGTSAQGYTGTCFEPRADAKGDIARIYFYFSVRYYNNSRVVAWESMNA
                     GAKLKTWAITLYKSWASADPVSSKERTRNNAIHAIQKNRNPFIDYPSLINLIDFAN"
     gene            819..2693
                     /locus_tag="PLD82_05450"
     CDS             819..2693
                     /locus_tag="PLD82_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64865.1"
                     /translation="MIAARELKNLRLFAMVIDVIPDMIGILDTAYTVVCYNKAVYAML
                     GEQPETVIGKKCYANVGRQEICAVCAVKECYKSKKVEIVERYDPDLRMWIELRAYPVF
                     DSDGQLMNVIEHFRDITQRKISETALIEQKQLLQSITDNVFEMIALTDMQGNFLFLGK
                     SHAILGYDLDWLIGRNVLEYVHPEDVERITREFAAFMGQSDAIPRVEYRYRCADGRYV
                     WLETVGTHISPSSGGECRLLFSSRDVTARRQIEQDLAESEERFKALHNASFGGIAIHD
                     KGVILECNLGLSHMSGFTMEELVGMDGLLLIAEDWRPLVRSNIQAVYERPYEAYGLRK
                     DGSRYPIRLEARNIPYRGKQVRVVEFRDITEQKQAEEHRRMLEAQLRQEQKLSAVGTL
                     ASGVAHEINNPLTGIINFAQLILDRLPGEVETIREWAGEILAEGDRIRTIVSNLLLFS
                     RQESGHRQRVAPESLVGSILSLCGNLLKKHEVQVEVHLDDDLPDLVCVQGQIQQVLLN
                     LIINARDALDDRHPGFHPDKKVYITGHGLHDGEGCWIRFIVEDHGGGMSAEVAARCLE
                     PFYTTKTGERGTGLGLSVSYGIVREHGGRLSFETEEGRGTRFFLDLPMGDAGSVRDGI
                     "
     gene            2794..3501
                     /locus_tag="PLD82_05455"
     CDS             2794..3501
                     /locus_tag="PLD82_05455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG64866.1"
                     /translation="MIDRSLNYGRKQIKKFLLRIPDVRHSLDLGAGRGDDLALVRAVF
                     PEADCHAVECRQEAALALAREGIDAAMLDIERERLPWADNSMDLVIINQVLEHVKDHF
                     WLLHETSRVLGPGGYLIVGVPNLASLHNRILLFFGRQPTPIANTSAHIRGWTTGDLGR
                     FLAIGGCYKKVGRRGANFYPFPALLARPLAWLFPGLAWGSMTLWQKRPDAEYGDAFVR
                     FPAEHRLETNFYTGGEV"
     gene            3651..>4082
                     /locus_tag="PLD82_05460"
     CDS             3651..>4082
                     /locus_tag="PLD82_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG64867.1"
                     /translation="MELKRQLGLFTAVCVIVGDMIGTGIFATTGFALADVGSAALVLG
                     LWIVGGLVAIAGALSYAELSSIWPEVGGEYVYLKNIFGKLPAFMTGWVSLFVGFSAAV
                     AASSLVMTGYLKEFLRNILGPEAGVVVLFSDPAFQKVFAIAV"
BASE COUNT          893 a          993 c         1234 g          962 t
ORIGIN      
        1 gcacatgatt ccccagagca agtttgccga agccgccccg atggtcagcg atgcccacca
       61 tgtgacagct actgacggca aggtcaacgg gatgcgctcg aattatccgc acggcaatgt
      121 tgcctcggct acttggactt cgaaaaacgg cggcaagctc ggcgctggca cctcggccca
      181 agggtatacc ggaacctgct tcgagcccag agcggatgcc aagggtgata tcgcccgcat
      241 ctacttctac ttctcggtcc gctattacaa caacagccgt gttgtagcat gggagtccat
      301 gaacgccggt gccaagctca agacttgggc gattaccctg tacaagagct gggcttcggc
      361 tgatccggtc agctcaaaag agcggacccg caacaatgcg atccatgcca tccagaaaaa
      421 ccgcaacccc tttatcgact atccttccct catcaacctg atcgactttg ccaactgatc
      481 aactgtgagg tacaaaaggc cgctccgaag ggggcggctt tttttttact gggacgtccg
      541 tggcggggca agagggcgca gtgattggaa aaatctttca cacttctcgt gcattgaaag
      601 acagggcagg atatcccgga tccggatact cattctcgct tgtgacagga tgggaaaatt
      661 gaagacgatg tcgcgtcaat attgaccgga gccacggtgg atctggccta tcttcaaacg
      721 gtcatcagtg ccaggatccg gaaatggata aatccttggc tctggtgtaa aatcaggatg
      781 ccatggacaa atattcgcct gaagtgccag agcaggtcat gattgctgca agagaactca
      841 aaaacctgag actctttgct atggttatcg atgtcattcc cgacatgatc gggattctgg
      901 ataccgcgta taccgttgtg tgctataaca aagccgtgta cgcgatgttg ggcgaacagc
      961 cggaaacggt catagggaaa aaatgctacg ccaatgtggg tcggcaggag atttgtgcgg
     1021 tttgtgccgt caaggaatgc tacaaaagca aaaaggttga aattgtggag cgctatgatc
     1081 cggatctccg gatgtggatc gagctccggg cctatccggt ttttgacagc gacgggcagt
     1141 tgatgaatgt gattgagcat tttcgtgata ttacacaacg taaaatcagt gaaacagccc
     1201 tgatcgagca aaaacagctt ttgcagagca tcactgacaa cgtctttgag atgatagcct
     1261 tgacggacat gcagggaaat tttctgttcc tcggaaagtc acatgccatc ctggggtatg
     1321 atcttgactg gttgatcggg cggaatgtgc tcgagtatgt ccaccccgag gatgttgaaa
     1381 ggataaccag ggaatttgct gcatttatgg gtcaaagcga tgccataccg agggttgagt
     1441 atcgctaccg ctgtgccgac ggacggtatg tctggttgga gaccgtggga acgcatattt
     1501 ctccatcgtc cggaggagag tgtcgtttgc ttttcagttc ccgggatgtg accgcccgca
     1561 ggcagatcga gcaggatttg gcagagagtg aggagcggtt taaggcgctc cacaacgcat
     1621 cctttggggg gatcgcgatt catgacaagg gggttatcct ggaatgcaat cttggtctct
     1681 cacatatgtc tggtttcact atggaagaac tggtgggaat ggatgggctg ctgctgattg
     1741 ccgaggattg gcggcctctg gtgcggagca atattcaggc tgtctacgaa cggccgtatg
     1801 aggcatatgg gcttcgcaag gacggatcgc gctatcccat acgattggaa gcccgcaata
     1861 ttccctatcg tggcaaacag gtgcgtgtgg tcgaattcag ggatattacc gaacaaaagc
     1921 aggctgaaga acacaggcgg atgcttgaag cccagctccg ccaggagcaa aagctctcag
     1981 cagtcgggac actggccagc ggggtcgcgc acgagatcaa taatccattg acggggatta
     2041 tcaattttgc ccaattgatt ctggatcgct tgcctggtga ggttgagacg atccgggagt
     2101 gggccggcga gattctggct gaaggcgacc ggatccgcac gattgtcagc aatctcctgc
     2161 tcttttcaag acaggagtcc gggcacaggc agagggtggc cccggaatcg ctggtcggct
     2221 ccattctctc gctctgcggg aaccttctca aaaagcatga ggtccaagtc gaagtccatc
     2281 tggacgacga tttgccggat ctggtttgtg tgcaaggaca gatccagcag gtcctgctca
     2341 acctgatcat caatgcgcgc gatgcattgg atgaccgtca tcccggattc catcctgaca
     2401 aaaaagtcta tattaccgga catgggttgc atgacggaga aggctgctgg atccgcttta
     2461 tcgtcgagga ccatggcggg ggaatgtcgg ccgaggttgc ggcccgttgc ctggagccgt
     2521 tctatactac gaagaccggt gaacggggga cgggcctcgg cctgtcggtc agttacggta
     2581 tcgtacggga acacggaggc cgtttgagtt tcgagaccga ggaaggtcgg gggacgcgtt
     2641 tctttctcga tctcccaatg ggtgatgcgg gatcggtacg ggacgggata tgaccatgcc
     2701 gtcggctgtg ggccgatgat tccggggcgt gtacgctatc ctgctgcagg cttgagggtg
     2761 ttcatcaaaa caaacatgaa ggagtcacgg cgcatgatcg atcggtccct gaattatgga
     2821 cggaaacaaa taaaaaaatt tcttctacgt atacccgatg tccggcattc ccttgatctc
     2881 ggggcggggc gaggtgacga tctggcgctt gtccgggccg tttttcctga agcggattgc
     2941 cacgcggtcg agtgtcggca ggaggcggcc ttggcgctgg ccagggaggg cattgatgca
     3001 gccatgctcg atatcgagcg ggaacggctt ccctgggccg acaactcgat ggatctggtg
     3061 atcatcaacc aggttctcga gcatgtcaaa gaccacttct ggctcttgca tgagaccagc
     3121 agagttctcg ggccgggcgg atatctcatc gtcggggtgc ccaatctggc ttcgttgcat
     3181 aacagaatat tgttgttttt tggtcgccag ccgacgccca tagccaatac cagtgcccat
     3241 atccggggtt ggacgaccgg ggacctgggc cggtttttgg ccatcggggg gtgctacaaa
     3301 aaggtcgggc ggaggggtgc gaatttttat ccgtttccgg cattgcttgc cagaccgttg
     3361 gcgtggctct ttcccggatt ggcctggggc agcatgacgc tttggcagaa acgacctgac
     3421 gcagagtatg gggatgcctt tgtccggttt ccggccgagc acaggcttga aaccaacttt
     3481 tataccggcg gggaggtgta agacgcacat ggtgtttgtg tgatattaca gggatgtaac
     3541 atactgcaga tggatccagt gcctctcttt aagttgtgga tggcggtcgt tgaccgattg
     3601 gttgtccagc gttagtttga gagaacgata taattcaagg aacgtactga atggaactca
     3661 aacgacagtt gggactgttt accgcggttt gtgtcatcgt tggcgacatg atcggaacgg
     3721 gtatctttgc gacgaccggg tttgctttag cggatgtcgg ttcggcggcg ctcgtcctcg
     3781 gactctggat cgtcggtggc cttgtggcca tcgccggcgc tctctcctac gccgagcttt
     3841 cctcgatctg gccggaggtg ggcggtgagt atgtctatct caaaaacatc ttcgggaaac
     3901 tcccggcctt catgacgggt tgggtctcgc tcttcgtcgg cttttcggcg gcagtggctg
     3961 catcatcgtt ggtcatgacc ggctatctga aagaattttt gaggaacatc ctcgggccgg
     4021 aagcgggtgt ggtggttctc ttttcggatc cggccttcca aaaggttttt gccatcgcgg
     4081 tg
//
LOCUS       DAOSHE010000158         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20020_length_4065_cov_4.532419, whole genome shotgun sequence.
ACCESSION   DAOSHE010000158 DAOSHE010000000
VERSION     DAOSHE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20020_length_4065_cov_4.532419"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLD82_05465"
     CDS             <1..485
                     /locus_tag="PLD82_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG64868.1"
                     /translation="LVDSITRLARAYNQVVPTSGKILSGGVDSNALHRPKRFFGAARN
                     IEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNMEIVLDRHLADRRIFPAIDINRSG
                     TRKEDLLLTESELAKIWVLRKVFASMNDIEIMELLIDKMRTSKTNEVFLRMMNSPGES
                     "
     gene            602..814
                     /gene="rpmE"
                     /locus_tag="PLD82_05470"
     CDS             602..814
                     /gene="rpmE"
                     /locus_tag="PLD82_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG64869.1"
                     /translation="MKQGIHPEYYQATVKCACGNTFVTGSTQKEIHVEICSACHPFFT
                     GKQKLVDSTGRVDRFKKRYNIANKKD"
     gene            825..2021
                     /locus_tag="PLD82_05475"
     CDS             825..2021
                     /locus_tag="PLD82_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOG64870.1"
                     /translation="MREPVAPIGGQALIEGVMMRSKPGYSVALLRKSGRVDVSWFDAV
                     SFAKKHPVFAKPFLRGMTALFESMVIGVRALMFSASEQEIEEEDEKQVKARDKAKKKA
                     EKLQLSGFTAGLMVAVSLVFGLGLFVALPNIIIELLGIKEVSDPFLFNLVSGVVRIVM
                     FVGYIALIALMKDVRRVFQFHGAEHKSVNCYEAGLPVTEANAKKFTTLHPRCGTSFMF
                     FVLMVAILLFAFVPVVLNATWPWFKSLAAGATLEIVIQKAIIIGLHILLLPVVSGIAY
                     EFIRFSWKFRSNASCRMLMAPGLALQKLTTREPDDLQIKTAVKALLEALKRQEMAVHG
                     VEVPGVTVRTKVASGSKKTASASGRGTSAPKKKAVVSTETGRTRKTPAKVVASVTKSP
                     GRAKKA"
     gene            2288..3142
                     /locus_tag="PLD82_05480"
     CDS             2288..3142
                     /locus_tag="PLD82_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG64871.1"
                     /translation="MTIGIREKRDLRNEDEDFIKLYFEEINRTPLLSFEEEKALSKKI
                     LKGDKQALENLIKANLRLVVKIAKQYVTKDYQIMDIIQDGNLGLIKAAEKYDYRRAVR
                     FSTYASWWIKQSIVRSLSLKKRMIRIPHRKEEKLRHIRKVWNSLNQSLRRIPTVDEIA
                     LELDMDIHEVDALLGMANPVVSFENTLASDDCALFNILEDYTFSPDHLVLQETIRRET
                     NEVLRSLVPKERMVLACRYGFNNDRKCTLKRMGDHFGISAETVRQIEMKALRKIREKH
                     AYLREYLD"
     gene            complement(3308..3502)
                     /gene="rpmB"
                     /locus_tag="PLD82_05485"
     CDS             complement(3308..3502)
                     /gene="rpmB"
                     /locus_tag="PLD82_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG64872.1"
                     /translation="MSRKCEICGKGSIVGNTISHSHRKARRSWLPNIRKIKVREENGT
                     VCTKAVCTRCLRSNKVTKAL"
     gene            3638..>4065
                     /locus_tag="PLD82_05490"
     CDS             3638..>4065
                     /locus_tag="PLD82_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64873.1"
                     /translation="MPSIDAIADKPVQTALRDAIRDADGHEVYFRGRFDASKKLVEVE
                     VLARGTVSSVAAGMPIKPPWNAVLHNHPGGVLHPSEADVSISAWLSIKEVAFLIINND
                     ATQYCAVTEPVIVHVPADIKAESVKKLGDHPLAREFLLDG"
BASE COUNT          990 a         1024 c         1096 g          955 t
ORIGIN      
        1 ttctggttga ttctattaca cgacttgccc gtgcctacaa ccaggtggtg cccacttcgg
       61 ggaaaatcct ctcgggcggt gtcgattcca atgcgcttca tcgccccaag aggttttttg
      121 gagcggcccg aaacatcgaa gaaggtggct cgctcacaat cattgccaca gccctgatcg
      181 agacgggcag caggatggat gaggtcattt ttgaggagtt caagggaacc ggaaatatgg
      241 aaatcgtcct tgaccggcat ctggccgatc ggaggatctt cccggccatc gacatcaacc
      301 gttccggaac gcgcaaggaa gacctgctct tgaccgagag cgagctggcc aagatctggg
      361 tcctgcgcaa ggtttttgcg tcgatgaacg atatcgagat catggaactc ctgatcgaca
      421 agatgcggac atcaaaaacc aacgaggttt tcctgcggat gatgaattca ccgggggagt
      481 cgtgacaggt tgtacaacgc gcatcttgac ttgaagggat gccgtatcta tcattacagc
      541 atgccttggg cacggagtcc atatcaatcc agggctgaca tattgaccag gagttcacca
      601 catgaagcag ggtatccatc ccgaatatta tcaggccacc gtcaagtgcg cctgcggcaa
      661 caccttcgtc actggctcaa cccaaaagga gatccatgtt gaaatctgct cggcctgtca
      721 ccccttcttc acggggaagc aaaagctcgt cgacagcacg ggacgtgtcg accgcttcaa
      781 aaagcgctac aatatcgcaa acaaaaaaga ctgattcacc cgaaatgcgg gagcctgtcg
      841 caccgattgg cgggcaggct ctcattgaag gggtgatgat gcgcagcaaa ccggggtact
      901 cggtcgccct cttgcgaaag agtggtcgag tcgatgtttc ctggtttgac gccgtttcct
      961 ttgccaaaaa gcatccggtt tttgccaagc cctttctccg cggaatgacc gctcttttcg
     1021 agagcatggt gatcggggta cgggcactta tgttttcggc ctccgagcag gagatagagg
     1081 aagaggacga aaagcaggtc aaggcaaggg acaaggcgaa aaaaaaggcc gaaaaacttc
     1141 agctttccgg ttttactgcc ggtctgatgg tggcagtctc cctcgtattt ggtctgggtc
     1201 tctttgttgc tttgccaaac atcatcatcg agcttctcgg tatcaaggaa gtaagtgatc
     1261 cttttctctt caacctcgtc agcggtgtcg tgcggatcgt catgtttgtc gggtatatcg
     1321 ccctgattgc cttgatgaag gatgttcggc gcgtgttcca gttccatgga gccgagcaca
     1381 agtccgtcaa ttgttacgag gccggtctcc cggtgacaga ggccaacgca aaaaagttca
     1441 cgacgctgca ccccagatgc ggaacaagct tcatgttttt cgttcttatg gtcgccatcc
     1501 tgctctttgc cttcgtgcct gtcgtgctca atgcgacttg gccctggttc aagagtctgg
     1561 cagccggagc gacgctcgag attgtgattc aaaaggccat catcatcggg ctccatattc
     1621 ttttgcttcc ggttgtgagc gggattgcgt atgagttcat caggttctcg tggaaatttc
     1681 gctccaatgc cagctgccgg atgttgatgg cgcccgggct ggctctccag aagttgacga
     1741 ccagagaacc ggatgacctg cagatcaaga ccgctgtcaa agccctgctg gaagccttga
     1801 aaaggcaaga aatggccgtc catggtgtcg aggttccggg cgtcacagtt cggaccaagg
     1861 ttgcgagcgg gtcaaaaaag actgcttcag cttcgggaag gggcacatcg gctcccaaaa
     1921 agaaagccgt tgtttcaacc gagacgggaa gaacacggaa aacccctgcc aaggtggttg
     1981 cctccgtgac caagagcccc ggaagagcca aaaaagcata ggaattctgt gtcgattatg
     2041 caacagtggc aggtgaaact ggcatgaatt cgacacaagt ttccacaaaa aaaacacggc
     2101 cagaccgtgt tttttttctg attggtgcta gtcttataaa tgttcacgtt tatggaattt
     2161 tcctttcggt gttaactttc aacaaaacaa taagatgtat gttcaggcaa tcatgtcctc
     2221 tccgcttggc acgtcaattg cagtttattc tcctgttgtg tgtgtagacc gcaccaaagg
     2281 agatcgcatg acaatcggga tacgggaaaa acgggacctg cgcaatgagg acgaagattt
     2341 catcaaattg tactttgagg agatcaacag aactccgctc ctttcgtttg aagaggagaa
     2401 ggctctttca aaaaagattc tgaagggtga caaacaggcg ctggaaaacc tcatcaaggc
     2461 caatttgaga ctcgtggtca agattgcaaa acaatacgtg acaaaagatt accagatcat
     2521 ggacattatt caggacggca atcttggtct gatcaaggcg gcggagaagt atgattatcg
     2581 cagggcggtc cggttttcga cctatgcctc ctggtggatc aaacagtcca tcgtacgcag
     2641 tctctccctc aaaaagcgca tgatccgcat tcctcatcgc aaggaagaaa agctcaggca
     2701 tattcgcaag gtgtggaaca gcctcaatca gagtttgcgc aggattccga cggttgatga
     2761 aatcgcactc gagctcgaca tggatatcca tgaagtggat gcgctcctgg gtatggccaa
     2821 tcccgttgta tcgtttgaaa acacgcttgc cagcgatgat tgtgcattgt tcaacattct
     2881 tgaggactat accttctcac ccgaccatct tgtgttgcag gagacgatcc gacgggagac
     2941 gaatgaagtc ctgcgatccc tggttccgaa ggaacggatg gtcctggcct gccgttacgg
     3001 gttcaacaat gaccggaagt gcaccctcaa acggatgggg gaccattttg ggatctcggc
     3061 cgaaacagtc cggcagatcg aaatgaaggc ccttcgcaaa atccgcgaga aacacgccta
     3121 tctcagggag tatctcgatt gagtgctctt tcatgtacag gctgacagga gaccgttgcc
     3181 gtgaggcagc ggttttttct ggaaaggctc cgatccatac ctggtattga tgtgggcagg
     3241 caagcgtgaa cctgctgata cataaaaaaa agaggccaag ggcctctttt tcgcggtact
     3301 cgaaaactta cagcgctttt gtgaccttgt tggaacgcaa acagcgggtg cagactgcct
     3361 tggtgcagac ggtgccgttt tcttcgcgga ccttgatctt gcggatgttt ggcaaccaac
     3421 tgcggcgagc cttgcgatgc gaatggctga tcgtatttcc gacgatgctg ccttttccgc
     3481 agatttcaca ctttctcgac atggcgaacc tcgtagctct tcattgccgg agaaggaaga
     3541 cacccacgac cggcacgaat tgaaaagtac acctggaatg ggtgaaaagt caatgaaaag
     3601 aacagaatcc ggcgtctttg ctccgtacat ttagggtatg ccttccatcg acgccattgc
     3661 cgacaaaccg gttcagactg ctctccgcga tgcgattcgc gatgcggacg gacacgaagt
     3721 gtatttccgg ggtcgctttg atgcttcaaa gaaactggtt gaagttgaag tcctggcgag
     3781 ggggaccgtt tcttccgtag cggccgggat gccgatcaag ccaccctgga atgctgtcct
     3841 gcacaatcac ccgggtggtg ttctgcaccc ctccgaagcc gatgtgtcca tttctgcatg
     3901 gctctcgata aaggaagtgg cttttttgat catcaacaac gatgccactc aatactgtgc
     3961 cgtgaccgag ccggtgatcg tgcatgtccc ggccgacatc aaggccgaat ccgtcaagaa
     4021 gctgggcgat catcccctcg ctcgagaatt cctgctggac ggatt
//
LOCUS       DAOSHE010000159         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20030_length_4064_cov_3.313046, whole genome shotgun sequence.
ACCESSION   DAOSHE010000159 DAOSHE010000000
VERSION     DAOSHE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20030_length_4064_cov_3.313046"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLD82_05495"
     CDS             complement(<1..812)
                     /locus_tag="PLD82_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG64874.1"
                     /translation="MELRQILSTKSACVESWLHGWMDGLGKAERYPRRLLEAAAWSLF
                     AGGKRLRPALCLLVNDACGSPEGRVLPLAGALEMIHTYSLIHDDLPPMDDDELRRGIP
                     TCHIKFDEATAILAGDALLTEAFACLATLDDRDVIPDIVSGLARAAGFGGMVAGQMAD
                     IGAERGYLPGDSATLEFIHANKTGALLEASVLLPGLVNHLDSDRIAELAAYGRSIGLA
                     FQIADDVLNETADAATLGKPTGTDREAGKLTWTSIHGIEKAREMANREVENA"
     gene            complement(784..1599)
                     /locus_tag="PLD82_05500"
     CDS             complement(784..1599)
                     /locus_tag="PLD82_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOG64875.1"
                     /translation="MKIIAVGDVVGRPGRQALLMTLPGLVKEHAPCCVIVNGENAAGG
                     FGISARIAEAFLTWGVDVITTGNHVWRNKDVFKIIETERRLLRPLNYPEGAPGKGSVL
                     VEKNGVQVGVINIMGRVFMDPLDCPFKAARREVHALKRAGAKIVVVDFHAEATSEKAA
                     LGHYLDGEASLVFGTHTHVQTNDPRILPGGTGFVTDVGMTGATDSVIGVMKEKAMRKF
                     VSGMPAKFDLAEGGVEINYAIAEIDQETGRTVSIGVVRRKVVLDDGAPADTLD"
     gene            complement(1669..3198)
                     /gene="rny"
                     /locus_tag="PLD82_05505"
     CDS             complement(1669..3198)
                     /gene="rny"
                     /locus_tag="PLD82_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOG64876.1"
                     /translation="MSDIVWIIISLSALAGGFLLRVILARLGLSSAEDKAKQVIENAR
                     KEADATRREAIIETKDAILRERNKFDNETRERRMEIKKMEDRLLSKEENLDRRLAGLE
                     KREKTVQQREQLAEDKIAELEVERGKVLKTLETMSGMTVEQAREMLVNDLREDARLEG
                     AKFVNKIESEARQNAEKKAREIIITAIQHCVPEVVGEATVSSVSLPSDEMKGRIIGRE
                     GRNIRALEVLTGVDVIIDDTPEAVVLSCFNPVNREIARLSLERLITDGRIHPAMIEEI
                     VTKVRRDIDKVIVEEGERTVFDLGLHSMHPELIKAVGRLKFRTSYGQNILNHSRAVAN
                     VCGMLAGEVGADVQKAIRGGLLHDIGKGIEMEGGGGHALIGADLARMYGEDDDVVNMI
                     AAHHGDKEPSCLETVLVSAADAISAARPGARKESLEGYIKRLENLEKIANDFKGVEKS
                     FAIQAGRELRIMVQNDLIDDEGARQLARDVAKRIEREIKHPGVVKVTVIRETRIVEHA
                     Y"
     gene            complement(3246..4064)
                     /locus_tag="PLD82_05510"
                     /pseudo
     CDS             complement(3246..4064)
                     /locus_tag="PLD82_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251425.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
BASE COUNT          822 a         1282 c         1102 g          858 t
ORIGIN      
        1 caggcattct cgacctcgcg gtttgccatc tcacgggctt tctcgatccc gtggatcgat
       61 gtccaggtca attttccggc ctcacgatcc gtccccgtgg gtttccccag agtggccgca
      121 tccgctgtct cgttcaagac atcgtcggca atctggaacg ccaacccgat cgaacggcca
      181 taggcggcca gttcggcgat cctgtcagaa tcgagatggt tgaccagacc tggcaaaaga
      241 acggatgcct caagcaaggc tcccgtcttg ttggcgtgga taaattccag cgtagcagag
      301 tcgcccggaa gatacccgcg ttcggctccg atatcggcca tctgtcccgc gaccatcccc
      361 ccgaatccgg ccgcccgggc cagtccggag acgatgtcgg gaatgacatc ccggtcgtcg
      421 agcgtcgcca gacaagcaaa cgcctccgta agcagtgcat ccccggccag aatggcggtg
      481 gcctcgtcaa acttgatatg gcaggtgggt atccccctcc ggagttcatc gtcatccatc
      541 ggaggcagat cgtcgtggat cagggagtag gtatggatca tctcaagtgc cccggctaga
      601 ggcaggaccc gtccttctgg agaaccgcag gcgtcgttga caaggagaca gagtgccggc
      661 cgaaggcgct ttccaccggc gaagagtgac catgccgcag cctcaaggag acggcgcgga
      721 tagcgctctg cttttcccag accgtccatc cagccatgaa gccagctctc cacacatgcg
      781 gatttagtcg agagtatctg ccggagctcc atcgtccaga accacctttc gtctgacaac
      841 tcctatcgag accgtcctgc cggtctcctg gtcgatttca gctatcgcgt agttgatttc
      901 gacaccaccc tcggccaaat cgaacttggc cggcatcccc gagacaaatt tccgcatcgc
      961 tttctctttc atcacaccga tcacggaatc cgtggcaccg gtcatgccga catctgtcac
     1021 gaacccggtt ccacccggaa gaatccgggg atcattggtc tggacatggg tgtgcgtccc
     1081 gaaaaccagg gaggcctccc cgtccagata gtgcccgaga gcggccttct ccgaagtggc
     1141 ttcagcgtga aaatcaacga ccacgatttt ggcaccggcc cgcttgaggg catggacctc
     1201 ccgccgggcc gccttgaacg gacagtcgag cggatccatg aagacccggc ccatgatgtt
     1261 gatgacgccc acctgaaccc cgtttttctc gacaagtacg ctccccttgc cgggagcccc
     1321 ttcggggtag ttcagcggac ggaggagacg cctttcggtc tcgatgatct tgaacacatc
     1381 cttgttccgc cagacatgat tcccggtcgt aatcacatcg acgccccagg tcaaaaaggc
     1441 ctcggctatc cttgcggaga ttccgaaacc accggccgca ttctcgccgt tgacgatgac
     1501 acagcagggt gcatgctctt tgaccagccc ggggagtgtc atcaacagag cctgacgacc
     1561 aggtcgaccg acaacatccc caaccgcgat gattttcatg aaaaactcca gatccggggg
     1621 atagggaacc cgcgccgtct tcaaatcgag tcgggcgcgg gtatccggtc aatatgcgtg
     1681 ttcgacaatc ctggtttcgc ggatgacagt caccttgacg acaccggggt gcttgatctc
     1741 ccgctcgatt ctcttggcga catcccgtgc caactgccgt gcgccctcat catcgatcag
     1801 atcattctgg accatgatgc gcaattcgcg tccggcctgg atggcaaagg acttttcgac
     1861 acccttgaaa tcgttggcga ttttctcgag attctccaga cgcttgatat agccttcgag
     1921 actctccttg cgggcaccgg gccgggccgc actgatggca tcggctgccg aaaccaggac
     1981 ggtctcgaga cagctcggtt ccttgtcacc atggtgggcg gcaatcatgt tgacgacatc
     2041 atcatcctcg ccgtacatcc tggccagatc ggcaccgatc agggcatgac cgccgccgcc
     2101 ctccatctcg atacccttgc cgatatcatg gagcagaccg ccacggatcg ccttctggac
     2161 atcggcaccg acctcgccgg cgagcattcc gcagacattg gcaaccgccc gggaatggtt
     2221 gaggatgttt tgcccgtagc ttgtgcggaa cttgagccgc ccgacagcct tgatcagttc
     2281 ggggtgcatg ctgtgcagac cgagatcaaa gacggtccgt tcgccctctt cgacgatcac
     2341 cttgtcgata tcacggcgga ccttggtcac gatctcctcg atcatggccg ggtgaatccg
     2401 cccatccgtg atcaggcgct caagggagag ccgggcaatc tcgcgattga ccgggttgaa
     2461 gcaggagagg acaacggcct cgggggtatc gtcgatgatc acgtcgacac cggtcaggac
     2521 ttcgagagca cggatattcc tgccctcacg tccgatgatc cggcccttca tctcgtcact
     2581 gggaaggctg accgaactga ctgtcgcctc gccgaccacc tcggggacac agtgctgaat
     2641 ggcggtgatg atgatctccc gggccttctt ctcggcattc tgcctggcct cactctcgat
     2701 cttgttgaca aatttggcgc cctcaagccg ggcatcctcg cgaagatcgt tcaccagcat
     2761 ctcccgggcc tgctcaacgg tcatgccgga catggtctca agggtcttga gcacctttcc
     2821 ccgctcgacc tcaagctcgg cgatcttgtc ctcggcaagc tgctcacgct gctggaccgt
     2881 cttttcacgt ttttcaagac cggccagacg acgatctaga ttttcttctt tggagagaag
     2941 ccggtcctcc atcttcttga tttccatgcg gcgttcgcga gtctcgttat cgaacttgtt
     3001 gcgctcacgc agtatggcat ctttggtttc aatgatcgcc tcgcgacggg tggcatccgc
     3061 ctccttgcga gcgttttcaa taacctgctt tgccttgtct tcggccgaag aaagccccag
     3121 acgggcaaga atgacgcgaa gcagaaaacc gccggccaga gccgacaggg aaatgatgat
     3181 ccagacaata tcactcatgt ttcactcctt tctcaatatt ttcttgtgct cgatcagcag
     3241 catattcatt ccacagtcac gctctttgcg agattgcggg gcatatcggg atcaaggccc
     3301 ttggccaccg tgatgtaata ggccaaaagc tgaagcggaa caacggtcag gaggggcgaa
     3361 agcggttcga tcacgtccgg aacatggaag acaacatcgg catgccgccg gatactctca
     3421 tctccctcgg tggcaatggc gatcacccgt cccttgcggg cccgaatctc ttcaatattc
     3481 gagatgacct tttcgtagac accatagcgg ggggccacta ccaccaccgg catccctttt
     3541 tcgatcaggg cgatcggccc gtgcttcatt tcggcagccg gatacccctc ggcgtgaatg
     3601 taggaaacct ctttgagttt gagagcgccc tcgagagcca ccgggaaatg gaacccacgc
     3661 cccagataag gcagttactc tgtcccttga actcgtccgc caaggctcgg atggccgaag
     3721 cctgttcgag cacgcgttcc atcttttcgg gaagccgtaa caactcctgc agaaaggcca
     3781 tcccctgact gccggacatc ttggacatcc gggccagtct gagggcaatc aaggccagag
     3841 ccgtcacctg ggaggtaaaa gccttggtcg aggcgacgct tttttcgggc ccggcgtgga
     3901 tgtagatccc gcatcccgac tcgcgagcga tcgtgctgcc cgtaacattg acgataccgg
     3961 caaccgttgc cccccgcatc ttggcttcac gcagtgccgc cagggcatcc gctgtctcac
     4021 cggactggga gatcacgaga accagtgtct ccggaccaat cacc
//
LOCUS       DAOSHE010000160         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20226_length_4037_cov_3.953541, whole genome shotgun sequence.
ACCESSION   DAOSHE010000160 DAOSHE010000000
VERSION     DAOSHE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20226_length_4037_cov_3.953541"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..877
                     /locus_tag="PLD82_05515"
     CDS             155..877
                     /locus_tag="PLD82_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG64877.1"
                     /translation="MNFLLVDTKTSLRDILIRKLFANGINLYYAETLEAAHKIISSTK
                     ITILLIDIDVYPTKAFAFLKMLGLLPSKPVRIVSSSLTDKKTILPFVNIGIAGWLVKP
                     FTEDKSLPKLMDIIRGITPADEQRTFFRVTPGKNDDRKVFFRLPSSAKLYSATLLNIS
                     AGGLALNTPEEIPAPELDLGVFISKIQIKLASQDMDLSGEVVYKSGTVFALRLKDCHE
                     RDMYILSKYIFDHISMTVHPVE"
     gene            complement(1137..2513)
                     /gene="secF"
                     /locus_tag="PLD82_05520"
     CDS             complement(1137..2513)
                     /gene="secF"
                     /locus_tag="PLD82_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOG64878.1"
                     /translation="MIKTNINFIKGRKYAYFFSAVVILVCLLSIFVFRGFNQGIDFTG
                     GLTANLTINFEKSDIGMMRSLFSAVTLHQVVGEETSSDRIRSAMERKPEAGAALSNAG
                     ATNAVVSNRAALPAGKTREIVENLGKNITRLEKKAGESTNRQSETYLIRIKNIPGVDK
                     DEIERALTGVLAKKFKQRTVVSVSIASKDANDDGTALSALLKPFGMDGELKGLVMNVS
                     EQGKTNRVKIFEASQTFETAVNETEIRNGLTKLFLAQPQYDISKAVEFSSTIEWGSFN
                     SVSSNIGKELQDSAIWLSLLCVLIMLVYIAIRFDFRFGVGAIVALIHDMIIMLGVVSL
                     TGNEMNVPVIAAVLTIFGYSINDTIVVFDRIRESLKFSRKEDLPGIVNRSINETLSRT
                     IITSATTLFAAVSIYLFAGEVLRDFAFSLIVGIVFGTYSSIFIASPMYLLMEKLTDKK
                     VSESASKA"
     gene            complement(2534..>4037)
                     /gene="secD"
                     /locus_tag="PLD82_05525"
     CDS             complement(2534..>4037)
                     /gene="secD"
                     /locus_tag="PLD82_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG64879.1"
                     /translation="KRKKVLNLGLDLQGGLYLVLAVDDKSLTEDLVSRYSADLKASTN
                     SAFRTMSATNPAFVREVAKRVDENFSREMDLAITSAYEKIKNRVDQFGVSEPLVEMGN
                     DQRIYVSLAGIKDPQAAEEIVSKAGRLTFQIVDEDLMKSFIDKYAQTRPELFSLVEHR
                     SRSGKSVEYLINVDGILPADFEVPEGTAVYWIWERDRFGTSKKKGGIFLKKEIVLDGK
                     YIDSAQPSFGQYSSEVHVSFTLKNTKLDEDKETGVEQLARVTGENVNKRMAIVLDGKV
                     QSYPVIKGRIPGGSGQITGNFTAEEAKNLSAILNAGSFKASLKVDEKRSVGPSLGRDS
                     IMAGVQAAMIGFVLIVVFMAVYYKYAGFLSDIALLLNFVIALAVMTQLQATLTLPGIG
                     GLILMLGMAVDANVLIFERIREEMRRGDATMRVAIDKGFGRAFMTIFDSNLTTLLSAL
                     VMMQMGSGMVKGFAVTLFIGILTSMFTALFVTRYLVDGSMSLFKIKRLPI"
BASE COUNT          916 a         1201 c         1056 g          864 t
ORIGIN      
        1 gggatgtttt ccgaattgat cccgctcccc cggctggcca agcggatccg cgagatcgtc
       61 aatatctccg aagcgaaaaa aaaggtggcg aaacccgtca agcaaccgca atgacttcgt
      121 tgcgccaacc gagattccga ccgaggtgta ccccatgaat tttcttctgg tcgacacaaa
      181 aacaagcctc cgggacatcc tgatccggaa gctcttcgcc aacgggatca atctctacta
      241 cgccgagacg ctggaagcgg cccacaaaat catctcctcg acaaaaatca ccatcctcct
      301 gatcgatatc gatgtctatc ccaccaaggc ctttgccttc ctcaaaatgc tcggactcct
      361 cccgagcaag cccgtccgga tcgtctcttc cagcctgacg gacaaaaaga cgattctccc
      421 cttcgtcaat atcgggatcg cgggctggct ggtcaaaccc ttcaccgagg acaaaagcct
      481 tccaaaatta atggacatca tccggggcat cacacccgcg gatgaacaac ggacattctt
      541 ccgtgtcacc cccggcaaaa acgacgaccg caaggttttt ttccggctac cctcgagcgc
      601 caagctctac agcgcgaccc tgctcaacat cagtgcgggc ggactggcgc tcaacacacc
      661 cgaagagatc cccgcccccg agctcgatct cggtgttttc atctccaaga tccagatcaa
      721 actggcatcg caggacatgg atctctcggg cgaagtcgtc tataagagcg gcactgtctt
      781 cgccctgcgg ctcaaggact gtcacgaacg cgacatgtac atcctttcaa aatacatctt
      841 cgaccatatc tccatgaccg tccatcccgt ggagtaatcc ccctctccgg tgcggaacct
      901 ccgggactcg cgtctcttcg cccgggatac cctgtattcc gggagcattg ttcatgatga
      961 acagcctcag cccgaccgga cgcggcacca gcccagaacg catcttcttc cggggccacc
     1021 gtcctgtacc gttcatttcc tggccatccc tgacattccg gatgacccat aaaaaaacag
     1081 gggtgccccg cttgagctgg agcacccctg ttgtctgtca ggaatgagga aagggatcag
     1141 gccttgctgg ctgactcact taccttcttg tccgtcaact tctccatgag gagatacatc
     1201 ggcgatgcaa tgaagatcga ggagtaggtt ccaaacacga taccgacgat cagcgagaat
     1261 gcaaagtcac gcaggacttc gccggcaaag aggtagatcg aaaccgccgc aaagagggtc
     1321 gtggccgatg tgatgatcgt ccgtgagagt gtttcgttga tactgcggtt gacaatcccg
     1381 ggaaggtctt ccttgcggga gaacttgaga ctctcgcgga tgcggtcaaa aacgacgatc
     1441 gtgtcattga tcgaatatcc gaagatggtc aacacagccg ctatcaccgg gacattcatc
     1501 tcgttccccg tcagggagac gacacccaac atgatgatca tgtcgtggat cagggccaca
     1561 atggccccaa ctccgaagcg gaagtcaaag cggatcgcga tatacacgag catgatcagt
     1621 acgcagagaa gtgagagcca gatcgccgaa tcctggagtt cttttccgat gtttgaagaa
     1681 accgagttga acgatcccca ttcgatcgtg gacgagaact cgaccgcctt gctgatgtcg
     1741 tactggggct gggccaaaaa cagcttggtc aatccgttcc ggatctccgt ttcgttgaca
     1801 gccgtctcga aggtctggct tgcctcgaag atcttgaccc ggttggtctt tccctgctcg
     1861 ctgacattca tcaccaggcc cttgagctcg ccatccattc caaaaggctt caggagtgca
     1921 gacaaggccg tgccatcatc gttggcatcc tttgaagcga tgctgacgga cacgaccgtg
     1981 cgctgtttga acttcttggc gagcacaccg gtcagggcac gctcgatctc gtccttgtcg
     2041 actccgggga tgtttttgat gcggatcaga taggtctcgc tctggcgatt ggtggattcg
     2101 cccgccttct tttcaagacg ggtgatgttc ttgcccagat tctcgacaat ctcgcgtgtc
     2161 tttccggccg gaagagccgc ccggttgctg acaacagcgt ttgtcgctcc ggcattcgag
     2221 agagcggccc cggcttcggg cttccgctcc atagccgagc ggatccggtc actgctcgtc
     2281 tcctcaccca ccacctggtg gagcgtgaca gccgagaaca aactccgcat catcccgata
     2341 tccgacttct cgaaattgat cgtcagattg gccgtcagac cgccggtaaa gtcgatcccc
     2401 tgattgaatc ccctgaagac aaagatggac aggagacaga caaggatgac aacagccgag
     2461 aagaaatagg cgtatttcct gcccttgata aaattgatgt tcgtcttgat catttcgctt
     2521 cgtccatcct ctctcaaatg gggagacgct tgatcttgaa gagactcatc gagccgtcaa
     2581 ccagatagcg tgtcacaaag agtgcggtaa acatcgaggt gaggataccg atgaagaggg
     2641 tcacggcaaa gcccttgacc attccgcttc ccatctgcat catcaccagt gccgagagca
     2701 gcgtcgtcag gttggagtcg aagatcgtca tgaaggcccg tccgaatccc ttgtcgatcg
     2761 caacccgcat ggtcgcgtca ccacgccgca tctcttcacg gattctctcg aaaatcagca
     2821 cattggcgtc cacagccata cccagcatca ggatcagacc accgataccc gggagcgtga
     2881 gggtcgcctg gagctgtgtc atcacggcca gggcgatcac gaagttgagg aggagtgcga
     2941 tgtcggaaag gaaaccggca tacttgtaat agacggccat gaagaccacg atcaggacga
     3001 agccgatcat ggcagcctgg acaccggcca tgatggagtc gcgacccagg gaaggtccga
     3061 cggatcgctt ctcgtcaacc ttgaggctgg ccttgaagct tccggcattg aggatggcgg
     3121 agagattctt ggcctcttcg gcggtaaaat tccccgtaat ctgtcctgac ccgcccggaa
     3181 tcctgccctt gatgaccgga taggactgga ccttgccgtc aaggacaatc gccatccgct
     3241 tgttcacgtt ttcaccggtc acccgggcca actgctcgac accggtctcc ttgtcctcgt
     3301 caagcttggt attcttgagg gtaaaagaaa cgtggacttc gctcgaatac tggccgaagc
     3361 tcggttgcgc cgagtcgatg tacttcccgt ccaggacaat ctcctttttc aggaagatcc
     3421 cgcccttttt ctttgacgtt ccgaaccggt cccgctccca gatccagtag accgcggtac
     3481 cctcgggaac ctcgaaatcg gccgggagaa ttccgtcgac gttgatgaga tactcgacag
     3541 acttcccgct ccgggacctg tgctcaacca gcgagaagag ctcgggccgg gtctgggcat
     3601 acttgtcgat gaaggacttc atcagatcct catccacaat ctggaaggtg aggcgccccg
     3661 ccttggaaac gatctcttcc gcagcttggg gatccttgat accggccaga ctgacgtaga
     3721 tccgctggtc attgcccatc tcgaccagtg gctcggaaac gccaaactgg tcaacccggt
     3781 ttttgatttt ctcgtacgcc gaggtaatgg cgagatccat ctcgcgggag aagttttcat
     3841 ccacccgctt ggcaacctcg cgcacgaagg cgggattggt cgccgacatg gtgcgaaatg
     3901 ccgaattggt cgaggccttg aggtcggcag aatagcggga cacaagatcc tcggtcagcg
     3961 acttgtcgtc aacagcgagg acgagataga gtccgccctg caggtcaagt cccaggttga
     4021 gcaccttctt gcgtttt
//
LOCUS       DAOSHE010000161         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20287_length_4030_cov_2.587673, whole genome shotgun sequence.
ACCESSION   DAOSHE010000161 DAOSHE010000000
VERSION     DAOSHE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20287_length_4030_cov_2.587673"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLD82_05530"
     CDS             <1..222
                     /locus_tag="PLD82_05530"
                     /inference="COORDINATES: protein motif:HMM:NF037860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64880.1"
                     /translation="PGRVIAIRGLEEALALRGVRHVFLNVDIGDVVVTPRSNVEKAGH
                     IIAVGETREDAIRIIEAAQKAIQIETGPL"
     gene            complement(232..747)
                     /locus_tag="PLD82_05535"
     CDS             complement(232..747)
                     /locus_tag="PLD82_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64881.1"
                     /translation="MIKSVLAILLVGLVLAACGGSSFSIVKEPKQDTEGFLSPDMLQI
                     VAVGYPAWKETDPVKRRQQATAAAETMARLRAVEYLMNELQNESAEMYQTVVMKIGSR
                     LTTERYDPVYGNELADGGRKADEYFSLLSISGYPHTNWYTSEDGRCLMVYRVVKAGLV
                     AFGQRGFGMYP"
     gene            complement(870..2132)
                     /locus_tag="PLD82_05540"
     CDS             complement(870..2132)
                     /locus_tag="PLD82_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64882.1"
                     /translation="MKKSLLAGFCLLILAAAPLASLTPGYGASEDVSLFMSSHENNNY
                     LLNNFKLWLRLDVSEEFSLYVRGKFQYARLLNAADGTASADTKQSVLDLDLGYLSWGG
                     EDLGIIAGRTLITLGSGLLFDGAADGLDITLSAGPVDFELFGAYTGFIVGDTNPYNFS
                     RQDISDGAKRVFAGLSCGLDIGSGWAVTLNGLVQSDRGDIEASRYDTAHLSLAVEAQF
                     SPAVTMLLEGAVEGGEGPESTGTSMRDISAWAVTFRMQFALLDESESAFAIDAAMATG
                     SDNRYESTPGGIDGDGKDTQFSTFGTYSTGFAYEPDLSNLVYGALSFTTLPFSRGSLF
                     GRSSFTLKSAVYFKADKAAPTSELATTDTLNNPDSAFLGFAVDATWAWRVVSDLSLVF
                     AAGIFKPGTAYTDRSLQSLIQTTLVFAF"
     gene            complement(2165..>2831)
                     /locus_tag="PLD82_05545"
     CDS             complement(2165..>2831)
                     /locus_tag="PLD82_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG64883.1"
                     /translation="VQGRVSIRSGASGPWVAARSGMTLPQGASITTGYQSAVTLKIAP
                     TGSVVRLNQFTTVSVASLGQSGNTVNTTLKLKMGTVRAVVKNTVNERSRFVVSTPVAT
                     ASVRGTIPEVSHFPDTGTRITYLQGSGYVLNKKGRLQLLARNQRSTVSTKGATSTALQ
                     ESGKRRAAAAIQLNLTPAEQDALFRPGRGRPGDPGMGGRDFIRDLQKWRYIRENLSDP
                     VML"
     assembly_gap    2832..2841
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2938..3417)
                     /locus_tag="PLD82_05550"
     CDS             complement(2938..3417)
                     /locus_tag="PLD82_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64884.1"
                     /translation="MKVLFGFMLCVFVFLSGTLAAQNAETNTRPEGMDGLMALSEKQT
                     VRIGEALEQIALLAGSEPRDARASLSFLAGKGIVSSDIALDAVLTRGCLAAMIMKIKG
                     WGGGVMYSLFGGGRYAWRELVYRNVMQAGGSEHSPVSGGELLAVIGRAAGDVELKME"
     gene            complement(3530..>4030)
                     /locus_tag="PLD82_05555"
     CDS             complement(3530..>4030)
                     /locus_tag="PLD82_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64885.1"
                     /translation="RSTMRWSVVFWLLPAVLAGCSFEFPFDQSIDLTTGRADLAVARA
                     VTNVNGEDVQKAVDGLSKFGEVESLALDGAWIQTNPGDDLSAVEYAEIILSNSSRMQR
                     YDVLNPSGTNASITLDGSVLLWFYQNPPFDVHAVLYRDEAGSVIPTHYRVRLKGRMLV
                     EALVKP"
BASE COUNT          940 a         1184 c         1132 g          764 t
ORIGIN      
        1 ccaggacgcg tcattgccat tcgtggactc gaggaagccc tcgccctgcg cggggtccgg
       61 cacgtctttt tgaatgtcga catcggggat gtggttgtca cacccaggag caatgtcgaa
      121 aaagccggtc acatcatcgc ggtcggagag acacgggagg acgcgatcag gatcatcgaa
      181 gcggcacaga aggcgatcca gatcgagaca ggcccactgt gagggatgtc gtcagggata
      241 catcccgaaa ccgcgctgcc cgaaggcgac cagtccggcc ttgacgaccc tgtagaccat
      301 caggcaccgc ccgtcctcgg acgtgtacca attggtgtgt ggatagcccg aaatcgagag
      361 cagggaaaaa tattcgtcgg ccttgcggcc accgtcagcc agttcattcc cgtagacggg
      421 gtcatagcgt tcggtcgtca agcggctccc gatcttcatg acaacggtct ggtacatctc
      481 ggcgctctca ttttggagtt cattcatcag gtattcaacg gcacgcagcc gtgccatggt
      541 ttcggcagcg gcagtagcct gttggcgacg tttgacaggg tccgtctcct tccaggcagg
      601 atacccgaca gccacgatct ggagcatgtc cggcgaaagg aacccctccg tatcctgctt
      661 gggttctttg acgatggaaa aagaacttcc accacaggcc gcaagaacga ggcccaccaa
      721 aagaatcgca agaactgatt taatcatgtc acccccggta ttcctgcggg ccagtgacgc
      781 cgcagagtgt ctccacaaga gaagcagaca cgcgacatcg gttcatcaag ccgatgaatc
      841 gtgcgcgcgt catcaaaaca tactccggat caaaaggcaa atacaagggt cgtctgaatc
      901 agggactgga gggatctgtc agtataggcg gtacccggct tgaagatgcc agcagcaaaa
      961 acaagggaga gatcactcac gacacgccag gcccatgtag catcaacagc gaaaccgaga
     1021 aaggcggagt ccggattgtt gagcgtatcc gttgtcgcca gttccgaagt cggagcggcc
     1081 ttgtcagcct tgaagtagac agcactcttg agggtgaaac tggagcgtcc gaacagtgag
     1141 cccctcgaga agggcaaggt cgtaaacgag agtgctccgt acaccaggtt cgaaagatcc
     1201 ggctcatacg cgaatccggt tgaatacgtg ccgaaggtcg aaaattgggt atccttgccg
     1261 tccccgtcaa tcccgcccgg ggtactctcg tacctgttgt cacttccggt cgccatggca
     1321 gcatcgatcg cgaacgcact ctcgctttca tcgagcaagg cgaactgcat cctgaaggtc
     1381 accgcccagg cactgatgtc gcgcattgag gtcccggttg attccggtcc ctcccctccc
     1441 tcgacagcac cttccagcag catggtgaca gccggagaga actgggcttc aacggccagt
     1501 gaaagatggg ctgtgtcata gcgggaggcc tcgatgtccc cccgatcgct ctggactagc
     1561 ccgttgagcg tcaccgccca gcctgaaccg atgtcaagcc cgcacgaaag acccgcaaag
     1621 actctcttgg cgccatcact gatgtcctga cgactgaagt tataggggtt ggtgtcaccg
     1681 acaatgaagc cggtatacgc accaaacagc tcgaaatcaa ccggcccggc ggaaagggtg
     1741 atatccagac catcagcagc cccatcaaag aggagaccgc tccccagcgt gatcagagtc
     1801 cgaccggcaa tgatcccgag atcttccccg ccccagctca gatagcccag atcgagatcg
     1861 agaaccgatt gttttgtatc cgcactggcc gtgccgtctg ctgcattcag aagtcgtgca
     1921 tactgaaact tcccgcggac gtacaaagag aattcctcgc tgacatccag gcggagccag
     1981 agcttgaaat tgttgaggag gtaattgttg ttttcatgcg acgacatgaa gaggctgaca
     2041 tcctcggaag caccataccc tggtgtcagg gacgcgaggg gagcggcagc cagaatgagg
     2101 aggcaaaagc ctgcaaggag tgattttttc ataaggagtc ccaaagtatc tgagttgcgc
     2161 ggtgtcacag catgactgga tcggaaagat tttcacggat atagcgccac ttctgtaaat
     2221 cacggatgaa atcgcgcccg cccatgccag gatcacccgg acgtccacgg cccggcctga
     2281 agagggcatc ctgctcggcc ggtgtcagat tgagctggat tgccgctgca gctctccgtt
     2341 ttcccgactc ctggagggca gtcgatgtgg cccccttggt cgagacagta ctgcgctgat
     2401 tgcgggccaa aagctgcagt ctgccttttt tattgaggac gtagcccgag ccctggaggt
     2461 aggtaatcct cgttcccgta tcagggaagt gcgagacctc gggaatggtc ccacggacgg
     2521 aagccgtagc caccggggtc gaaacgacaa agcgggaacg ctcgttgacc gtatttttaa
     2581 caacggccct cacggttccc atcttgagtt tgagcgtcgt attgacggta tttcccgact
     2641 ggcccagcga ggccaccgat acggttgtaa actggttgag ccgcacgaca ctcccggtcg
     2701 gagcgatctt gagggtcacc gccgactggt accccgtggt gatggatgcc ccctggggca
     2761 gggtcatacc gcttcgggca gcgacccagg gcccactggc gcctgaacgg atggaaaccc
     2821 tgccctggac cnnnnnnnnn nacggtcgcc gttgcggcgg cttcaagaga aagagcggcc
     2881 aatgcaaaaa aacacatcga aaaaacgaat attcttttca tatgggagcc tcccgaccta
     2941 ttccattttc aattcaacat caccggcggc ccgaccgatc acggccagga gttctcctcc
     3001 ggaaaccggg ctgtgttccg aaccgccggc ctgcatgaca ttccgataca cgagttcccg
     3061 ccaggcgtag cggccacccc cgaagagcga gtacatgaca ccgccacccc agcccttgat
     3121 cttcatgatc atcgcagcaa ggcagcccct ggtcaaaaca gcatcaaggg caatgtcgga
     3181 actgacaatc cccttcccgg cgagaaagga cagggatgcc cgtgcatccc ggggctcact
     3241 ccccgcaagg agggcaatct gctcgagcgc ttccccgata cggactgtct gtttttcgga
     3301 gagagccatc aggccatcca tcccctccgg ccgggtattc gtctccgcat tctgggcggc
     3361 gagggttccc gaaagaaaca caaaaacgca cagcatgaaa ccgaacaaca ccttcatacc
     3421 gtttgacctc tttttcattt gcgatatatg aatactactc tcccggcttc ctgaccgcaa
     3481 gccgaaatcg attcaagcag agcctgcacg accggcggct ccagcaggcc tagggtttga
     3541 caagagcctc gacaagcatc cgtcccttga gcctgacgcg atagtgggtg ggaatgacgc
     3601 tccccgcctc atcacgatac agaacggcgt ggacatcgaa aggaggattt tgatagaacc
     3661 agaggaggac ggatccgtcg agcgtgatgg atgcgttggt ccccgagggg ttgagaacat
     3721 cgtagcgttg catcctgctc gaattggaaa gaatgatttc ggcgtactcg acagcggaga
     3781 gatcatcgcc gggattggtc tggatccagg ccccatccag ggcaagcgac tccacctcac
     3841 cgaacttgga gagtccgtcg acggcctttt ggacatcctc gccgttgaca ttggtcacgg
     3901 cccgggcgac ggcaagatcc gccctgccgg tcgtgagatc gatggactgg tcaaagggaa
     3961 actcgaaact gcaccctgcc aacacagccg gaagaagcca gaaaaccacg ctccacctca
     4021 ttgtactcct
//
LOCUS       DAOSHE010000162         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20299_length_4028_cov_3.390637, whole genome shotgun sequence.
ACCESSION   DAOSHE010000162 DAOSHE010000000
VERSION     DAOSHE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20299_length_4028_cov_3.390637"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLD82_05560"
     CDS             complement(<1..423)
                     /locus_tag="PLD82_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG64886.1"
                     /translation="MTLLENIPLAPWTTFKLGGPARWYCPAEKPDDILEALAFAREHT
                     IPWFILGRGSNVLVSDHGYNGLVIHLAGLESVDWDCSGGTDLESPARQRVRCGAGLAI
                     AALAASAADRGLSGLEFAGGLPGSVGGAVYMNARAYGSE"
     gene            complement(584..3364)
                     /locus_tag="PLD82_05565"
     CDS             complement(584..3364)
                     /locus_tag="PLD82_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64887.1"
                     /translation="MNEYDEQSVLSRFTELTPSLLCLVSRTGAILYHNKTWTQRTGKA
                     VASPGEDLITDFMPQTHRDRFREALAAACISLGGDNRCNIVSDLLCADGNTIPVEWHI
                     SPDRDHAYLIGHDISRQRSADAERIRNQERLASSERNFRTFFETVNDIIVVATPQGEI
                     LYANPALKKKLQYDDATLGKMHVLEMHPGEHRQEAEEIFAAMFRGERDFCPVPLVAST
                     GQRLPVETRVWFGEWDGQVCIFGISKDLSAQHAALDRFHKLFNSNPALMAVSSLPQRR
                     FIEVNEAFLAHLGYSRQDVIGQTAADLQIFAEPEKQEMIAGILASGKHVRNIELLVRT
                     KTGKLLSGLFSGEIINNQVEDAFLTVMVDITELKQTENRLRESTRQLEIANKQARELA
                     EKAEAANKAKSSFLANMSHEIRTPMNGIIGLTDLLLDSDLNEKQLATLRSIQASGHAL
                     LEIINDILDFSKIESGKLDFENIVFSIHELFESCDEILAPRARDRSIGWEIVVAPDVP
                     RWAQGDPGRLRQIVLNLAGNAIKFTEKGSVTITVGLGRSSDPSRIRLFIAVEDTGIGI
                     PRDKIDLLFSSFQQVDASTSRRFGGTGLGLAISKRLVSMMDGEIGVESTVGKGSRFWC
                     TLCLGQTSGEGLTERLSKPALVTPELIEQRSRYRLLLAEDNGTNRQVALGILDKLGYR
                     ANSAANGHEVLYALSLLPYDAILMDIQMPEMDGFEATRAIRQHPDPTIAAIPIIAMTA
                     HAMRGDQDRCLAVGMNGYVSKPVAPQALADALDGLLLSPGNNAPVQSFAETPSSPPQG
                     PETVQDPPGEDPGQPPVFDMPALMARLFDDEELLTAIGEKFLAEMPQQIGELALAAEN
                     HDCTLCTKLAHRIKGASASVGGMRLAALMARIEEMAAHSATPDFSWISAPLSNEFARL
                     KEAISQAIRPRA"
     gene            3560..>4028
                     /locus_tag="PLD82_05570"
     CDS             3560..>4028
                     /locus_tag="PLD82_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG64888.1"
                     /translation="MTETQKHAAAFKQIIKENKQFLLLTHLDPDGDALGSACGLAYIL
                     KKMKKQVAIGLSEPFPARYEFLNRDESLRFVQHERVNPAKFDAVIVLDASTPSRLDKF
                     EGQIRNLAPESVLVNLDHHGDNSRYGTLNIVDTDAASASQMVIELLGARALDVN"
BASE COUNT          806 a         1118 c         1201 g          903 t
ORIGIN      
        1 ttcgctgcca taggcccggg cgttcatgta gactgccccg ccgacggagc cgggcagtcc
       61 tccggcaaat tccaggccgg aaagtccccg gtcggctgcg gatgccgcca gggcagcaat
      121 ggccagcccg gcgccacagc gaaccctttg ccgggccggt gactcgagat ccgtcccccc
      181 tgagcagtcc cagtcgacag actcgagtcc cgcgagatgg atcaccaggc cattgtatcc
      241 gtgatcggag accaggacat tcgaacctcg tcccagaatg aaccagggta tggtgtgttc
      301 gcgggcaaag gccaaggcct cgagtatgtc atcgggcttt tctgccgggc agtaccagcg
      361 ggccggaccg cccagtttaa aggttgtcca tggggccagt gggatgtttt cgaggagcgt
      421 catgctcttc aagatactgc cggactgcca gtgcggcgtg aaaagtcacc atggtgggtg
      481 gattccggcc ggtggtgctg gccaaatacc ggcaggctgg ctaaaaatcg gcaccatggt
      541 gctgcatatt gccaaaaagt caccatggtg ggcggcctgc tcctcaggcg cgcgggcgga
      601 tcgcctgact gatggcttct ttcaatcggg caaactcgtt cgacagtggg gcagagatcc
      661 aggagaaatc cggtgttgcg ctgtgtgccg ccatctcctc gatgcgggcc atcagggctg
      721 cgagtctcat cccgccaaca ctggcggatg cccccttgat gcggtgtgcc agctttgtgc
      781 agagtgtgca gtcatggttc tctgcagcca gggcgagttc cccgatctgc tgcggcatct
      841 cggcgaggaa tttttctcca atagctgtaa gaagttcttc atcatcaaag agtcgggcca
      901 tcagcgcggg catgtcaaag acgggtggct ggccgggatc ctctccggga ggatcctgca
      961 cggtctcagg accctgaggg ggtgaagacg gtgtttccgc aaagctttgt acaggcgcat
     1021 tgttccccgg ggacaggagg aggccatcca gggcatctgc cagcgcctga ggggcgaccg
     1081 gtttcgagac atacccgttc atcccgacgg ccagacagcg gtcctggtcg ccacgcatgg
     1141 catgtgccgt catcgcgata atgggaatgg cggcaatcgt ggggtcagga tgctgccgga
     1201 tggcccgggt tgcttcaaac ccgtccattt caggcatctg aatgtccatc aggatggcat
     1261 catagggcag aagacttagc gcgtagagaa cctcatgccc gttggccgca gagtttgccc
     1321 tgtacccaag cttgtcaagg atacccaggg caacctgccg gttggtgccg ttgtcttcgg
     1381 ccaggaggag acggtagcgg ctccgctgtt cgatgagttc cggtgtgacc agtgccggtt
     1441 tcgagagacg ctctgtcagg ccctcgcccg aagtctgccc gaggcagagc gtacaccaaa
     1501 agcgggagcc ctttcccacg gtactctcaa ccccgatctc gccgtccatc atggagacaa
     1561 ggcgcttcga aatggcgagg cccagtccgg tcccgccaaa gcgccgggat gtcgaggcgt
     1621 cgacctgctg gaaggatgaa aagaggaggt cgatcttgtc cctgggtatt ccgatcccgg
     1681 tgtcctcaac ggcgataaac aggcgtatcc gggaggggtc actggatctc ccgagcccga
     1741 cggtaattgt aaccgagcct ttttcggtaa atttgatggc attgccggca aggttcagga
     1801 cgatctggcg taatcgtcca ggatcaccct gtgcccagcg ggggacatcc ggcgctacca
     1861 cgatctccca gccgatggac cggtcccgtg ctctcggtgc caggatttcg tcgcagcttt
     1921 caaaaagttc gtggatcgaa aacacgatgt tttcgaaatc cagcttgccg gactcaatct
     1981 tggaaaagtc caggatgtca ttgatgattt ctaaaagggc gtgcccgctt gcctggatgg
     2041 aacgcagggt tgcgagctgt ttctcgttca ggtcactgtc cagcaaaagg tcagtcagtc
     2101 cgatgattcc gttcatcggg gtgcggatct cgtgactcat gtttgccagg aaactgctct
     2161 tggccttgtt tgccgcctct gctttttcgg ccagttcacg ggcctgcttg tttgcgatct
     2221 caagctggcg tgttgattcg cgcaacctgt tctccgtctg cttgagttcg gtgatgtcca
     2281 ccatgacagt caggaatgca tcttcaacct gattgttgat gatttctcct gaaaagagtc
     2341 cgcttaacag tttccccgtt tttgtccgga caagcagttc gatattcctg acgtgttttc
     2401 ccgacgccag aatcccggcg atcatctcct gtttttccgg ctcggcaaag atttgcaaat
     2461 cggccgcggt ctgcccgatg acatcctgtc gggagtatcc aagatgtgcc aaaaaggctt
     2521 cgttgacttc aataaagcgg cgttgtggaa gactgctgac cgccatcagt gccggattgc
     2581 tgttaaagag tttgtggaat cggtccaggg cggcatgctg ggctgagagg tctttggaga
     2641 ttccgaagat gcagacctgc ccgtcccatt cgccaaacca gacccgggtt tcaaccggaa
     2701 gacgttggcc ggtggatgca acaagtggga cgggacagaa atcccgttcc ccgcggaaca
     2761 tggcagcaaa gatctcctcg gcttcctgcc tgtgttcgcc ggggtgcatt tcgaggacat
     2821 gcatcttgcc cagtgtggcg tcatcgtatt gcaacttttt cttgagggcc ggattggcat
     2881 acaggatttc tccctgcggg gttgcgacca caatgatgtc attgacggtt tcgaaaaacg
     2941 ttctgaaatt ccgttcgctg gatgccagtc gttcctggtt tctgatacgt tcggcatccg
     3001 cgctgcgttg ccgggaaata tcatgcccga tgagataggc gtgatccctg tccggtgaga
     3061 tatgccattc gaccggaatg gtgttgccat cggcacaaag aagatcgctg acgatgttgc
     3121 accggttgtc acccccgagg gagatgcagg ctgcggcaag agcctcacga aacctgtccc
     3181 ggtgagtctg cggcatgaaa tccgtgatca gatcctcgcc gggactggcg actgccttgc
     3241 ctgttcgctg cgtccaggtt ttgttatggt acaggatggc gccagtgcgt gataccaggc
     3301 agagcaggct cggtgtcagt tcggtaaaac gggacaggac ggattgttca tcatattcgt
     3361 tcatgaccgg cacccctgtt ccctgataat ccatctatct tggcacactt tctccggacc
     3421 ggcaaggagc ttcaagggga tgtcgaatgc gcccggaaag aaggattgtc cacggcaacc
     3481 tgcagtccac cgggtttctc gctgaaaacc gccctgaggg gggtggacag aagggtcgat
     3541 tcaggatagc atcaagggta tgaccgagac acaaaaacat gccgccgcat tcaagcagat
     3601 catcaaggag aacaagcagt ttctcctgct gacccatctc gatccggatg gcgatgctct
     3661 cggctcggcc tgcggcttgg cctacattct caaaaagatg aaaaagcagg ttgcgatcgg
     3721 gctctctgag ccttttccgg cccgctatga gtttctcaat cgggacgaga gcctcaggtt
     3781 tgtgcagcat gagcgggtca atccggccaa gttcgatgcc gtcatcgtcc tcgatgcctc
     3841 gactccgtca cgtcttgaca agttcgaagg ccagatcagg aatcttgcgc cggagagcgt
     3901 tctcgtcaat ctcgatcatc atggtgacaa ttcccgctat ggaactttga atatcgtgga
     3961 cacggacgcg gccagtgcct cccagatggt cattgaactt ctgggggcac gtgccctgga
     4021 tgtcaatt
//
LOCUS       DAOSHE010000163         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20397_length_4014_cov_3.736297, whole genome shotgun sequence.
ACCESSION   DAOSHE010000163 DAOSHE010000000
VERSION     DAOSHE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20397_length_4014_cov_3.736297"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..358)
                     /locus_tag="PLD82_05575"
     CDS             complement(149..358)
                     /locus_tag="PLD82_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64889.1"
                     /translation="MADEQDRTAMSRRNWLLVLAGLGLFVLGMIVMVLVGKNHHGLSG
                     FLAPFFLVSGLVVCAVGMLIPPKQA"
     gene            complement(358..1317)
                     /locus_tag="PLD82_05580"
     CDS             complement(358..1317)
                     /locus_tag="PLD82_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64890.1"
                     /translation="MTGCRIVTGLSACCAGLCVAGAGEWFSPGVVGAGELFATLIPGA
                     LAAFLRPRLDMFSGLVGGYAGGLALLHPNLAVLPLAVWFVSAVVGVTGLFWILRTLIC
                     SGGWASVTEAGKVVFAVTGLFFASPLLVFLFSGTELQAGLPWTVRPILPGVDGLLVAI
                     LLPVLLGVPAGMGIMGLRRGRLLVLGALAFLAFFPLGEALPQTERSWRVLVASTAMMM
                     LAGSLLVGRFSPRMLDAARLAGASRLDIMSRLYLRASGWKLLWLFLAATVFAFDLREL
                     PAANPWASFFRILFSGLLLLYFGGLLPVHPVAGRGTKPDRRSE"
     gene            complement(1314..3146)
                     /locus_tag="PLD82_05585"
     CDS             complement(1314..3146)
                     /locus_tag="PLD82_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64891.1"
                     /translation="MRSPTMPGNRRFGPAFFFVGIVLVLFPAIFVLHQWETGSVRVDA
                     EIRAGWLARAVWLRLEDARREIPGVLVKTNWQGKPAAAWVPMPWFDGRVSLIRDDKPV
                     LPLKETGTTRAPVWVLPEKESGFFFARTDRSSVSPRLPVIGLDAFLLGQSPVKGLQLS
                     PDTGKREQGPVDGRIFFERQRRLDALAREVDALAGLGDRDAASALQAFSDGVAMYVQS
                     RGLPLPTVLYGEYSALSAAGHPDGQVRIVCGHPDTTGAVVVDRAIETIPAWAERILLV
                     APVGILALALFLTLRPRMSERSRVLILAVTVLVSSWMLWDDVRGRQDARIRGQVEYRE
                     LLLTKHAESGAVIARAAPSEWKAFPLLASSNLGHSGDTGLPLLILSSLLPGAILVIFL
                     RRLENPKVCAVHFMSLVPLVLVSGIFLHSLVDAFPAWTTSADGGGTGFLLLMFAALSL
                     QTVLAIPVVIAWTHMGKAWRSVLLALGALPLAVPLAFQDLLASGERLPWNLAEVFLLV
                     EGYPLVVVAMLMARTRIPFTVRQALALECGSVWRRFWSLDLWVCLLGAVPAWAISAWL
                     VLLHVEAAERPPMPLLIAFSLLLLGVALRVAPHWIRRGGTMEDS"
     gene            complement(3153..3689)
                     /locus_tag="PLD82_05590"
     CDS             complement(3153..3689)
                     /locus_tag="PLD82_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64892.1"
                     /translation="MSRGVLGVVLVGVAVLGLVAAGGSEWSNAQYKCAFRLPDDQWKN
                     TTEEKMGILKDGIVEFMSLSDFSVVSLTPYAFATGEQWKSEMTLKTVETTESMIRALS
                     DRVWLIREDPRYVVNGVSGYKREIGFQMNGIDYTAIIVVLSKNNTHYRLLFTSLEKFY
                     GSSFQNFLRIVGTFKITG"
     gene            complement(3814..>4014)
                     /locus_tag="PLD82_05595"
     CDS             complement(3814..>4014)
                     /locus_tag="PLD82_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64893.1"
                     /translation="DPPYDSVGLYVNLTTSGLARLVRGQLVLEIRAGGVPPAFPGLSV
                     TENRRYGDASLVFYSGEQTTDG"
BASE COUNT         1048 a         1240 c         1100 g          626 t
ORIGIN      
        1 gcgtcatcgg ccagagcggt aaaggcacca cccaacgaga gcgccttgac acccggcgag
       61 aacggatcgg atggatacca gtcagcggca agagggaggg aaacaacgag cagaatcccg
      121 agggcaagaa tccccctccc tggagccctc atgcctgttt tggcgggatg agcatcccca
      181 cagcacagac cacaagccct gatacgagga agaaaggcgc caagaagcct gagagcccat
      241 ggtgattttt tccgaccagc accatgacaa tcattccgag aacaaaaagt cccaatccgg
      301 cgagaaccag caaccagttt ctccggctca tggcggtcct gtcctgttcg tctgccatca
      361 ctccgacctc ctgtcaggtt ttgttccgcg gccggctaca ggatgcaccg ggaggagacc
      421 cccaaaatat agcaaaagaa ggccggagaa cagtattctg aagaaactgg cccagggatt
      481 ggctgcgggc aactccctga ggtcgaacgc aaacactgtg gcagcgagga acaaccagag
      541 cagcttccag cccgaagccc taagatacag ccgggacatg atatccagcc gggatgcgcc
      601 tgccaggcgg gctgcatcga gcatgcgcgg agaaaaccgt cccaccagga gactgcccgc
      661 caacatcatc atcgcagtgg atgcaaccag cactctccag gagcgctctg tctgcgggag
      721 ggcctctccc agggggaaga aggccaggaa ggcgagagcg ccaagcacaa ggagcctgcc
      781 acgcctgagt cccatgatcc ccatcccggc aggcacaccg agcaggaccg gaagcaggat
      841 cgcaaccagc aaaccgtcaa cccccggcag gatcggtcgg acggtccagg gcaggccagc
      901 ctgcaactcc gtcccggaaa acagaaaaac caggagggga gaagcaaaaa acaagccggt
      961 tacggcaaag acaaccttgc cggcttcggt gacgcttgcc cagccaccgg aacagatcaa
     1021 ggttctcaga atccagaaga gaccggtcac gccaaccaca gccgatacga accagaccgc
     1081 cagagggagc acggcaaggt tcgggtgaag cagcgccaat ccgccggcat atccgccaac
     1141 cagtcccgaa aacatgtcca ggcgaggacg aaggaaggcg gccagggccc ctggaatgag
     1201 tgtcgcaaag agttcccccg ctccgaccac tcccggcgag aaccactccc cggccccggc
     1261 cacacacaat ccggcacaac aggctgaaag ccccgtaaca atccggcaac cggtcatgaa
     1321 tcctccatgg ttccgcctct tctgatccag tgcggagcga cacgcagcgc cactcccaga
     1381 aggagcaggg aaaaagcgat caggagtggc atgggtggtc gctccgctgc ttcaacatga
     1441 agcagaacaa gccaggcact gatggcccag gcaggaacgg ccccgagcag acagacccaa
     1501 agatcgaggg accagaacct gcgccagacg ctcccgcact ccaaagccaa tgcctggcgc
     1561 acggtaaacg ggatgcgagt ccgtgccatg agcatggcca caacaaccag cgggtacccc
     1621 tcaacgagca aaaatacttc ggcaaggttc catggtagcc tctcgcctga agcaagcagg
     1681 tcctgaaaag ccaatgggac agccagaggc agtgcgccca gcgccagcaa tacactgcgc
     1741 caggcttttc ccatgtgcgt ccaggcgatc accaccggaa tggcaagaac cgtctggaga
     1801 gacaaggcgg caaacatcag cagaagaaag ccagtcccgc ctccgtcagc cgaagttgtc
     1861 caggcaggga acgcatccac gagggaatgc agaaagatgc ccgacacaag gacaagagga
     1921 acaagagaca tgaaatggac cgcacagacc ttggggttct cgagtctgcg caaaaaaatg
     1981 acgagaatgg cacccggaag cagggatgac aaaatcagca agggaaggcc cgtatcccct
     2041 gaatgaccga gattggagga tgcaagcagg ggaaaggctt tccattcgga cggagcagcc
     2101 cgggcaatga cagcaccgga ttcggcatgc tttgtcaaga ggagttcccg atactcgacc
     2161 tgcccccgga tcctcgcatc ctggcggccc cggacatcgt cccagagcat ccaggaagag
     2221 acgagaacag tcactgcgag gatcaggacc ctcgatcgtt cactcatcct cgggcgcagt
     2281 gtgagaaaaa gcgccagcgc caggataccg acgggagcaa ccagcagaat cctttcggcc
     2341 caggccggta ttgtttcaat tgccctgtcc acaacgaccg caccggtcgt atccggatga
     2401 ccgcaaacaa tccgtacctg accgtccgga tgaccggcag cagagagggc cgagtactcg
     2461 ccatagagta ccgtcgggag aggcaggccc cggctctgga catacatggc aaccccatcc
     2521 gaaaaggcct gcagagcgga ggctgcatca cgatccccca aaccggccag tgcgtctact
     2581 tccctggcaa gagcatccag acgacgctgc cgctcgaaaa agatacggcc gtcaacggga
     2641 ccttgctccc gcttgccggt atccggactc agttgcagcc ctttgaccgg tgattgaccc
     2701 aggagaaacg catccaggcc gataacggga agtctcggag aaacggaaga tctgtcggtc
     2761 ctcgcaaaaa agaacccgct ctctttttct ggaagcaccc agacaggtgc gcgggtggtg
     2821 ccggtctcct tgagcgggag aaccggcttg tcatcccgga tcagagaaac tcgaccgtcg
     2881 aaccatggca tgggaaccca ggccgcggcc ggtttcccct gccaattcgt tttgacaagc
     2941 acacccggaa tctcccggcg cgcatcctcg agccggagcc agaccgcacg ggccagccaa
     3001 ccggcccgga tttccgcatc tacccggacg gatccggtct cccattggtg cagaacgaaa
     3061 atcgccggaa aaaggacaag aacgattccc acgaaaaaaa aggcgggacc gaaacgccgg
     3121 ttgcccggca tcgtgggtga acgcactgca ccttacccgg ttatcttgaa agttccgacg
     3181 attctcagaa aattctggaa actcgatcca taaaattttt cgaggctggt gaagaggagc
     3241 ctgtaatgcg tattgttttt gctgagtacg acaatgatgg cggtataatc gattccattc
     3301 atttgaaacc cgatttcccg tttgtatccg gaaacaccgt tgacaacgta tctggggtct
     3361 tcccgtatca gccagaccct gtcggaaagg gccctgatca tgctctcggt tgtctcgacc
     3421 gtcttgagcg tcatttcgct cttccactgc tctccggttg cgaatgcgta tggagtcagg
     3481 gataccaccg aaaaatccga aagcgacatg aattcgacga ttccgtcttt caggatgccc
     3541 atcttctctt cggtcgtgtt tttccactgg tcgtccggga gtctgaatgc acatttatac
     3601 tgggcattgc tccactcgct tcctccggca gcaaccagcc caaggacggc aactccgacc
     3661 aacactaccc caagtactcc tcgtgacata catcctcccg tacaacttca ttggtcgccc
     3721 caaaaaacca cacgtgtact ctaacaccgg ttgacaaggg aaatcaaccg gaacaacacc
     3781 ggagaacaca cttctcgcga aaatccggcc gagtcacccg tcagtcgtct gttccccgga
     3841 ataaaacaca agggatgcat ccccgtaccg gcggttttcg gtcacagaga gaccgggaaa
     3901 cgccggagga acaccaccgg ccctgatttc aagcacaagc tgaccgcgaa ccagtcgagc
     3961 caatcccgaa gttgtaagat tgacatacag cccgacggaa tcgtatgggg gatc
//
LOCUS       DAOSHE010000164         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20616_length_3986_cov_4.448232, whole genome shotgun sequence.
ACCESSION   DAOSHE010000164 DAOSHE010000000
VERSION     DAOSHE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20616_length_3986_cov_4.448232"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLD82_05600"
     CDS             complement(<1..181)
                     /locus_tag="PLD82_05600"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG64894.1"
                     /translation="MDERLELRVVEYGEARTLLRQRLLHISRDQNTIETVAAILSDVA
                     ARGDAAVLEYTNRFDG"
     gene            complement(185..898)
                     /locus_tag="PLD82_05605"
     CDS             complement(185..898)
                     /locus_tag="PLD82_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64895.1"
                     /translation="MGEYHHGHMSKEELQDNALLSILVKIRHSLFKNKKLVYGVGAGV
                     LVILAVIIIMAINSSSAEKQASIEIDKVLVLLQAENARENVGDIDKKLDEIVKKWPDT
                     LAGAKAALHLANLQFKLDNFTGAIENFLKVVAYSKSGYLYPAALIGIGNCHEQSGDLV
                     KAIEYYDVVGQLVEHYGFRDMALLGKARILGAQGKIDEARKALEGLRSDVSSFADEAM
                     RLSIWLDSKKTVQVIPAGK"
     gene            complement(928..2607)
                     /locus_tag="PLD82_05610"
     CDS             complement(928..2607)
                     /locus_tag="PLD82_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG64896.1"
                     /translation="MSAQPEVFDQLAEMEKAQPQELHRGQIVDGLVVQIDDSFVWVDV
                     DSKSEGRIPRDEFETLPEEGSTIAVRLERSEDGIPVFSYKKAASEMGRRKLKTAFEEG
                     AIIRGKVVREVKSGVRVDFGGIEGYVPMRLLDIRSVQSAEYYIGQEFDFKIIEFAQGQ
                     RGRNSLSIVASRRELLEAEREHKTDEVFTKYRVGDEVEGVVRNITKSGVFVDIGGFDG
                     LIRLDDLAWKRGVNPRDIVSPQQTIKVRIVQMDPETKRVGLSLKEMTADPFVQFCDEV
                     REGDVIPGTVVKLENFGAFVRLRDGIEGLLHISELSWTRRIGHPKEVLTVGSTIDVKI
                     LGIDYDERRISLGFRQLQENPYDSLERDYAINSRHTGRISRLTEFGIFVELPNGVEGL
                     VRKEDIGWERGASNDLESRFVPGEMVEVRVINLDVRKRKIGLGIKQLQENPLEVFRFS
                     HPVGSRMSGTVTRLTDFGAFVDVGNSLEGLIHISNLAEKRVERVADVVKVGDTVEVKV
                     IEIDSEKNKIGLSIKDLERDTERDAINQHRGGEESGTFRLGDVLDLSKLSD"
     gene            complement(2631..3296)
                     /gene="cmk"
                     /locus_tag="PLD82_05615"
     CDS             complement(2631..3296)
                     /gene="cmk"
                     /locus_tag="PLD82_05615"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG64897.1"
                     /translation="MRTKPIIAIDGPAGAGKSTIARKIAAALNFVYIDTGAMYRALTL
                     RLLRSRTDPADLAGVRSILETVRIGFDPSGKIFLDGEDVSGTLRTAEVNSMVSTVAGL
                     PAVRESMQKLQRETGSEGGVVLDGRDIGSAVFPDAEYKFYLDASLEERARRRMNDSKE
                     KVFADLEAIQQDIARRDAADSQREHSPLVRAEDAQYIDTTGMGPEMVVSAMLEYIDRR
                     SVR"
     gene            complement(3278..>3986)
                     /locus_tag="PLD82_05620"
     CDS             complement(3278..>3986)
                     /locus_tag="PLD82_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG64898.1"
                     /translation="VDSKSLPAGIRGAFVCTTVEKIPEIALELLSVLPQECFVTDVGS
                     TKAGIVQRLARDQLAAGRFVGSHPMAGSEKSGYRNASPDLFQDATVVVTPAGRTRPDL
                     LERVRMLWTGLGARVVMMDAELHDRIVGMTSHLPHILSFTFAATLGWGKTAIGDYHRV
                     WGNGLRDMIRLAGSDPALWSGILMENRSAILEALNGYRKELETFSRLLKAGEQNACTG
                     YLEKCRTEMEAICEPNR"
BASE COUNT          916 a         1136 c          983 g          951 t
ORIGIN      
        1 caccatcaaa gcggtttgta tattcgagaa ctgcagcatc accacgagct gccacatctg
       61 agagaatagc ggcaaccgtc tcgattgtat tttggtcccg tgaaatatgc aagaggcgct
      121 gccgcaaaag cgtccgcgcc tccccgtatt cgacgacccg taattcaagt ctttcgtcca
      181 tgacttactt tcctgcgggg atcacctgca cggttttttt cgaatcgagc cagatagaaa
      241 gacgcatcgc ttcatcggca aacgatgaga catcggagcg aaggccttca agagccttgc
      301 gagcctcgtc gattttaccc tgggcaccca ggattctggc cttgcccaaa agtgccatgt
      361 cacggaatcc ataatgctcg accaattggc caacgacatc gtaatattcg attgccttga
      421 cgagatcacc ggattgctca tgacagtttc cgatgccgat aagagctgcc ggatagagat
      481 agccggattt gctgtacgcg actactttca agaaattttc gatggcacct gtgaaattgt
      541 cgagtttgaa ctggagattg gccaagtgca aggctgcctt ggccccagcc agggtatccg
      601 gccatttttt gacaatttcg tccagcttct tgtcgatatc gccgacgttt tcgcgggcat
      661 tttctgcctg tagcagcacg aggaccttgt caatctcgat tgaagcttgt ttttcggcgc
      721 ttgatgaatt gatggccatg atgataatga cagccaagat cacgagtact cctgcgccga
      781 ctccgtacac caacttcttg tttttgaaca gggaatgcct gattttgaca aggatagaga
      841 gaagcgcatt atcctgaagt tcttccttgc tcatatgacc gtgatgatac tcacccatct
      901 tcttctcccg tccctgtcgt gttcgtttca gtcagaaagc tttgaaagat cgagaacatc
      961 acctagcctg aaggttccgc tctcctctcc accacgatgc tggttgatgg catcccgctc
     1021 agtatcacgt tcgagatcct tgatggaaag accgatcttg tttttctcgg aatcgatttc
     1081 gataaccttg acctcgactg tatccccgac tttgacaaca tcggccacac gttcaacccg
     1141 cttttcggca agattggaga tatggatcag gccttcaaga gaattcccga cgtcgacaaa
     1201 tgctccgaaa tcggtcaagc gtgtcacagt cccactcatc cgggaaccga ccggatggct
     1261 gaaccggaaa acttcaagcg gattctcctg gagctgcttg attccaaggc cgatcttgcg
     1321 tttccggaca tcaagattga ttacacgaac ctcgaccatc tcgccgggaa caaaacggga
     1381 ctcgaggtca ttggatgcac cccgctccca gccgatatct tccttgcgga ccaagccttc
     1441 gacaccattg gggagctcga cgaaaattcc gaactcggtc aatctgctga tgcgaccggt
     1501 atgccggcta ttgatggcgt agtcccgttc aagactgtcg taggggtttt cttgaagctg
     1561 cctgaaaccg agagaaatcc ggcgttcatc gtaatcgatt ccgagaatct tgacgtcgat
     1621 cgtggaacca acggtcagaa cttccttggg atgcccgatc cgtctggtcc aggagagctc
     1681 ggagatatgg agaagcccct cgatcccgtc tcggagacgg acaaaggcac caaaattctc
     1741 gagcttgacg actgttccgg ggatgacatc cccttcgcgg acttcatcgc agaattggac
     1801 aaagggatct gcagtcatct ctttgaggga gagaccgacc cgttttgtct cgggatccat
     1861 ctggacaatc cggaccttga tcgtctgctg gggactgacg atgtcgcggg gattgacacc
     1921 gcgcttccag gcaagatcat cgagccggat caggccatca aaaccaccga tatcgacgaa
     1981 aacaccgctt ttggtgatgt tgcgtacaac accctcaacc tcgtcaccga cccggtactt
     2041 tgtaaaaacc tcatcggttt tgtgctcacg ctcggcctcg agaagttcac ggcgcgatgc
     2101 gacaatcgag agcgaattcc tgccgcgctg accctgagcg aactcgataa tcttgaaatc
     2161 gaattcctgg ccgatgtaat actccgcgga ttgcacggac ctgatatcca gcaaccgcat
     2221 ggggacatag ccttcaatcc cgccaaaatc gacacgaacg ccgctcttga cttcgcggac
     2281 aaccttgcca cgaataatgg caccttcttc gaaggcggtc ttgagtttgc gtcgacccat
     2341 ctcggaagcg gcttttttgt acgagaagac agggataccg tcttcgctcc gttcgagacg
     2401 gaccgcaatc gtagaaccct cttcggggag ggtctcgaac tcatccctgg gaatccttcc
     2461 ttcggacttg gaatcaacat cgacccaaac aaaggaatca tcaatctgca ccacgagtcc
     2521 atcgacgatt tgacccctgt gcagctcttg cggttgagct ttttccatct ccgcaagttg
     2581 gtcgaagact tccggctgcg ctgacatgtt ttccaaagga ggctccttgt ttaccttacg
     2641 gaacgacggt cgatgtattc gagcatggct gaaacaacca tctcgggacc cattccggtt
     2701 gtgtctatat actgtgcgtc ttctgcccgc acaagaggac tatgctcgcg ctgcgaatct
     2761 gctgcgtcgc gtcgggcaat atcctgttga atcgcttcaa gatccgcaaa taccttttcc
     2821 ttgctgtcat tcattctgcg gcgggcacgc tcttcgaggg aggcatcaag gtagaatttg
     2881 tactctgcgt caggaaaaac agcactcccg atatcgcgtc catccagtac aactccgcct
     2941 tcactccccg tctctcgctg aagcttttgc atggactcac ggacggccgg caggccggct
     3001 accgtcgaga ccatgctgtt cacctcggcc gttctcagtg taccagatac atcctcaccg
     3061 tcaagaaaaa tcttaccgga agggtcgaaa ccgatacgca ctgtctcgag aatcgaacgg
     3121 acccccgcaa gatcagcggg gtctgttctg ctccggagaa gtctgagcgt aagagcacga
     3181 tacatggcac ccgtgtcaat atacacaaaa ttcagggctg cggcaatttt ccgtgctatt
     3241 gtgctttttc ccgcacctgc tggcccatca attgcaatta tcggtttggt tcgcatatgg
     3301 cctccatttc ggtccggcac ttctcgaggt aacctgtgca ggcattctgt tctcccgctt
     3361 taagcaaacg ggagaatgtc tccagttctt tccggtatcc attcaatgct tcgaggatgg
     3421 cgctacggtt ctccatgagt attccgctcc agagtgcagg atcactcccg gccaaccgga
     3481 tcatgtcccg caaaccgttt ccccagacac gatggtagtc accgatcgct gttttccccc
     3541 atcccagggt tgccgcaaag gtaaacgaga gaatatgcgg aagatggctt gtcatcccga
     3601 cgatccgatc atggagctcc gcgtccatca tgacaacgcg agctcccagc ccggtccaga
     3661 gcatgcggac tctttcgaga agatctggcc gtgtcctgcc agcgggtgtg accacaaccg
     3721 tcgcatcctg aaacagatca ggggacgcat tcctgtatcc cgacttttcg gaaccggcca
     3781 taggatggga tccgacaaat cggccggccg caagctgatc cctggccagt cgttggacga
     3841 taccggcctt ggtgctgccg acatcggtaa caaaacactc ttgaggaaga accgacagga
     3901 gttcgagagc gatttcagga atcttctcaa ccgttgtaca gacaaaggct ccccggatac
     3961 cggccgggag actctttgaa tcgaca
//
LOCUS       DAOSHE010000165         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20617_length_3986_cov_4.431951, whole genome shotgun sequence.
ACCESSION   DAOSHE010000165 DAOSHE010000000
VERSION     DAOSHE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20617_length_3986_cov_4.431951"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..468)
                     /locus_tag="PLD82_05625"
     CDS             complement(268..468)
                     /locus_tag="PLD82_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG64899.1"
                     /translation="MELEVLSRRELEVLQHLANGGTSRSIADILGISVRTVESHRAHI
                     MEKLGLKNTASMVRFAIDHKLL"
     gene            complement(937..1299)
                     /locus_tag="PLD82_05630"
     CDS             complement(937..1299)
                     /locus_tag="PLD82_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64900.1"
                     /translation="MARILIVDDEKNIRFTLNEFLKDDNHDVHTADDAFSALELLKKQ
                     PVDLIICDIMLPRIKGTDLLFTVRAVYPDVKLLLITGQPSQETEEIGKKAGAFAYLPK
                     PVTKEMIRSVVKDALASP"
     gene            complement(1369..2457)
                     /locus_tag="PLD82_05635"
     CDS             complement(1369..2457)
                     /locus_tag="PLD82_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64901.1"
                     /translation="MESILVVDDEKNIRLTISEFLRDRKYTVHLAEDAMDAVEVLRTT
                     PVDLVITDIMMPRMKGTDLLDHIREEYPGIKTILITGQASLENATASLRGGAFDYLQK
                     PVTKNTLLSVVENACRILTLDRLNQRLMEENRQYREHLEDLVHRKSAHLTKLSQKIVT
                     VQDDERREVAHEIERTIGQSLIALKLDLQSVLKGIGSTESDALLDHLDAIITRTRSMA
                     STLDPVSIASTDLILVARRIREELGQSMGCRIEIEGDEPTTVDTMEQAGTVGLIIREA
                     VTNAIRHGRAGLITIQVRKDEDSVTLEIRDDGCGFGADTLGDDRGIGILLMQERTGVL
                     HGALTIHTTPGEGSVVSLTFPQKGPKTR"
     gene            complement(2562..3662)
                     /locus_tag="PLD82_05640"
     CDS             complement(2562..3662)
                     /locus_tag="PLD82_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOG64902.1"
                     /translation="MAQILLLHSSRKFRDRWTQIAEGEGHLVREAESVQEAKKILEKH
                     ASPCPVVVAGSDEIAEYNGETTAGIIELKKSAPCLCLVLVAPDPSIDEIASCIRAGAI
                     DYLAESVEPSEIRRSLNAAVNRAATEDLNRKLEAEHRLYQEHLESLVDRHTADLRSII
                     TRLIRIRDAEREEISVELHDQIGQDLLALKLQIQSLESAGDGSIRASTIIDRIDELAS
                     RVRLISHRLSPLFVQSAGIMTTIQILCNQCAASAGLVLQTKIDALENLPCETQVQSAI
                     HRFLEEALSNVAKHSRANTASLEIQVFPHEVDIILRDNGCGFDTTKETGGLGILLLGE
                     YASRLEGVYTLESAPGKGTIVTLHIPRKKFGV"
BASE COUNT          809 a         1003 c         1171 g         1003 t
ORIGIN      
        1 tgtatgtccg tcgagcctga ggaattcggc caggacattg cggatattct cctcgtcgtc
       61 aacaacaaga atccgccagg gcaggtcagc aggccgggcc tcgatcgtct cgggcagggt
      121 ataggttgtc ataatgttgt ctctccggta tgcgggccgt cctggttaaa tatccggaaa
      181 gcatgcaatt ctgtcaatcc ggacaaatac tgtcttcctc ggtatttata ctgagaaatg
      241 gcggtgtttt gtggattggt atgcggctta caggagtttg tggtcaatcg caaaacggac
      301 catggaggcc gtgttcttca atccgagctt ttccatgatg tgggcccggt ggctctcgac
      361 tgtccggaca ctgatcccga gtatgtccgc aatcgagcgg cttgtcccgc cgttggccag
      421 gtgctgcagg acttcgagtt ctctccgtga aagaacctcc agttccatcg acactccctg
      481 aggagtagag gtctccgaca accagagagg caagcttggg agaataggct ttttggccgg
      541 cctggatggc attcagggcc cagagaaggc tctcgtacac atcttctttg agaatgtatc
      601 cgtcaactcc ccggctgacc gcttgacgga agtactcatg actgttgtgc attgtcagaa
      661 gaaggacgcg gatccgagga tagtcttttt tgatgacttc cagagcctgg aagccgttca
      721 tgaccggcat cgagaggtcc atgagaatga tgtcgcaggg gatttgcttg aggaggtcga
      781 gcaattcctg tccgtctccg gcttcaccaa caacatggaa tcccggatca tccgagatca
      841 gctttcgcaa tccgagacgg aggatggcat gatcatccgc aaggaggatc ctgcatggct
      901 gatgctgcgt ttgtggcgaa ggcataggca aatgaatcag ggagaagcca gagcgtcttt
      961 gacgacactg cggatcattt ccttggttac tggcttggga agataggcaa aggcgccggc
     1021 ttttttgccg atttcctcgg tttcctggct gggttgtccg gtaatcagga ggagtttgac
     1081 atcgggatag acggcacgaa cggtaaagag gagatccgta cccttgattc tggggagcat
     1141 gatgtcgcag atgatcagat cgacaggctg ttttttcagc aattcgagag cggagaatgc
     1201 atcatcagcc gtatggacat catgattgtc gtccttgaga aattcattga gcgtgaagcg
     1261 gatatttttt tcatcatcaa caatgagaat acgtgccatg tctgcctctt cattcggtga
     1321 agaatacagc aactatacac ctgataatca ttgaaccgca atacatattt accgggtttt
     1381 cggtcctttt tggggaaagg tgaggctgac gacactgcct tcacccggtg tggtgtggat
     1441 cgtcagggca ccatgaagga cgcctgtccg ttcctgcatc agaaggatcc cgattccgcg
     1501 gtcatctccg agggtatccg ctccgaagcc acaaccatca tcgcggattt cgagcgtgac
     1561 actgtcctcg tctttccgta cctggattgt gatcaggccg gctctcccgt gtcggatggc
     1621 gtttgtcacc gcctcccgga tgatcagtcc cacggtgccg gcctgttcca tcgtgtcgac
     1681 cgtggtgggt tcatcacctt caatctcgat gcggcagccc atggattgcc cgagttcttc
     1741 ccggattctt cgtgcgacga ggatcaggtc ggtactggcg atggaaacag gatcaagggt
     1801 gcttgccatc gaccgtgtgc gcgtgatgat tgcgtcgaga tggtcaagga gtgcgtcgct
     1861 ttcggtggaa ccgattccct tgagaaccga ctgcaagtcg agtttgaggg cgatcaggga
     1921 ttgtcctata gtgcgttcga tctcatgagc cacttcgcgc cgttcgtcat cctggacggt
     1981 cacgattttc tgcgaaagct tggtcagatg tgccgatttt cggtgcacca aatcctcgag
     2041 gtgttcccga tactgcctgt tttcttccat taaacgttga ttcaaccggt caagggtcag
     2101 gatgcggcat gcgttttcga cgacggaaag cagcgtgttc ttggtgacag gtttttgcag
     2161 gtagtcaaag gcacctccac ggagggaagc ggtcgcgttc tccagcgatg cctggcctgt
     2221 gatcaggatg gttttgatgc cggggtattc ctcccggata tgatccagca agtcggtccc
     2281 tttcatgcgg ggcatcatga tatcggtaat cacaagatcg acgggggttg tccgcagaac
     2341 ctcgactgcg tccatggcat cctcggccag gtgcacagtg tattttcggt cgcgaagaaa
     2401 ctcgctgatg gtgagccgga tgttcttttc atcatcgacc acaagaatgg attccatgct
     2461 ctcccctgtt ctttgccgga acgcgcatat atgtcgatga tagctcctgg aatattcgct
     2521 gtccatccgt agaagtacgg aaaacactga aaatccttgt gttacacgcc gaattttttg
     2581 cgggggatgt gcagggtgac gattgtccct ttaccgggcg cgctctcgag cgtgtagacg
     2641 ccttccagcc gggaggcgta ttcgcccagc aggaggatcc cgagtccccc ggtttctttc
     2701 gtcgtgtcga atccgcatcc gttgtcccgc aggataatat ccacctcatg cggaaagacc
     2761 tggatttcaa gagatgctgt attcgcccgg ctgtgttttg ccacattgga caaggcttcc
     2821 tcgagaaaac ggtgaatcgc gctttgaacc tgggtttcgc atggaagatt ctccagtgca
     2881 tcgattttcg tttggagaac caatccggct gaagccgcac attgattgca gagaatctgg
     2941 atggtggtca tgataccagc agactggaca aagagcggag agagtcggtg cgagatgagg
     3001 cggacacggg atgccagttc gtcgatcctg tcgatgatgg tacttgcccg gatcgagccg
     3061 tctcccgcag attcaagaga ctggatttgg agtttgagtg ccagaagatc ctgcccgatc
     3121 tggtcatgga gttccacaga gatttcttcc cgctctgcat ccctgatgcg gatcagacgg
     3181 gtgatgatcg aacggagatc tgcggtatgt ctgtccacca ggctttcgag gtgttcctgg
     3241 tagagtctgt gttctgcttc gagcttccgg ttgaggtctt ccgtggctgc cctgttgaca
     3301 gcggcattca ggctgcgccg gatttcgctc ggttcgaccg attcggccag atagtcaatg
     3361 gcgcctgccc tgatgcagga ggcgatttcg tcgatggatg gatcgggtgc gaccaggacc
     3421 aggcagaggc atggtgcgct ttttttcaac tcgatgatgc cggccgtggt ttcgccattg
     3481 tactccgcga tctcatccga tcccgcgacg acaacagggc agggagatgc atgcttttcg
     3541 aggatctttt tggcctcttg gacggattcc gcttcgcgga caaggtggcc ctcgccttcc
     3601 gcaatctgtg tccagcgatc ccgaaacttc cgggaggaat gcaacaggag aatttgtgcc
     3661 atggaactcc gctccgtcag tcttgaacga gtgtatcacg gagtgggccg cagtgcaaat
     3721 ttcataatct ggagggtgtg tcgggttgat ccggcttgcg ggcaggcttt ggagacaggg
     3781 tttgatattc gggaacaaga tacgctccgg ccacgtcggt acacaggtcg aggacaatca
     3841 tctcgtgcgg gtatcgcgaa ccggtagcct ttagcgtcgc gggctgccat ccggatttcg
     3901 ccaagggttc cggctgtttt tcaaccagcg ttggtagaac gagcgtgccg actgggtgct
     3961 ctcgggtggt ctgaaggtgg ctgccg
//
LOCUS       DAOSHE010000166         3955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20866_length_3955_cov_3.990769, whole genome shotgun sequence.
ACCESSION   DAOSHE010000166 DAOSHE010000000
VERSION     DAOSHE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20866_length_3955_cov_3.990769"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLD82_05645"
     CDS             <1..508
                     /locus_tag="PLD82_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64903.1"
                     /translation="MLDEYDTAYGMLRGVPLELVGLWGCLRFAAEGLGCALLPWLQRR
                     GILTSLRSMGFWSAIAALILAAGIAGPDLLLPAYFAFFAMLAPVEIAFGGQIQRRIPD
                     TGRASFTSLVSLLTAGAAILIGLVLGAAASRGGLHLLFATGAGLVLLASLAGLFGRPV
                     NQDSTEGR"
     gene            complement(625..1464)
                     /locus_tag="PLD82_05650"
     CDS             complement(625..1464)
                     /locus_tag="PLD82_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOG64904.1"
                     /translation="MAKDVQKQLVVLVTGASAGIGRETVRLLLEAGHAVWASARNPKK
                     VADLVSVGARAVALDVENESSVKKALATILKRDGRIDVLINNAGYGSYGTVEDVPLDE
                     ARRQFEVNVFGLARLTQLVLPGMRERGSGRIVNIASIAGHVSVPGGAWYHASKHALVA
                     ITDALRMETRPFGIRVVMVKPGLVKTDWSRIALESMERYSGRGAYARLVSAMKDFFGK
                     GGTSVHKVARVVVRAALVRRPPVSWAVPFEAKAILFFRRIFPDRMVDAVMQHRLKFRK
                     EKK"
     gene            complement(1532..3226)
                     /locus_tag="PLD82_05655"
     CDS             complement(1532..3226)
                     /locus_tag="PLD82_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG64905.1"
                     /translation="MAVANETGTNAGGETPEAVDFIRKAVAEDLASGRFSGVHTRFPP
                     EPNGYLHIGHAKALCIDFGMAREFGGKCNLRFDDTNPVKEDTEYVDAIMEDIHWLGFD
                     WEDRLFYASDYFGQLCEYAVQLIRAGKAYVCDLTPDQIREYRGTLTVPGRPSPWRERS
                     VDENLDLFARMKAGEFPDGSRTLRAKIDMASPNINLRDPVMYRILHAPHHRTGDTWKI
                     YPMYDFAHGQSDSIEGITHSLCSLEYEIHRPLYDWFCEALGIFHPRQIEFARLNLTYT
                     VMSKRKLLELVKGGSVAGWDDPRMPTLCGLRRRGYTPESIKDFCDRIGVNKMDSTVDI
                     ALLEHCLREDLNKRALRRMAVLDPVKLVITNWPAGKVEMMEADNNPEDEAAGKRMVPF
                     SGELWIERDDVMENPPPKYFRLAPGREVRLKHGYYVTCTDLVKDADGRIAEVRCTHDP
                     ASRGGWTDDGRKVMGTLHWVSCSESVDLEIRLYDRLFAVEKPESVEDGVDYKTNLNPD
                     SLGVIRQAKGEPSLAEAPAGSQYQFLRQGYFAADPDSRPEKPVFNLAVRLKDSWKK"
     gene            complement(3301..3837)
                     /locus_tag="PLD82_05660"
     CDS             complement(3301..3837)
                     /locus_tag="PLD82_05660"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HOG64906.1"
                     /translation="MSFGDGTALFHEEQKFRNPWILLPLGLMACAALGFAIWALVRQL
                     GLGLPAGSKPVDNTTLILITSVQVVVWGSVFALIWFARLITEVHPDGVYLRYRPFHLK
                     PRFIPLGETDRVEVRDYHPVREYGGWGLRRGWKKRAYSVSGRQGLEILRDDGRRIMIG
                     TQRPAEMAAAVARIHQNR"
BASE COUNT          784 a         1237 c         1158 g          776 t
ORIGIN      
        1 catgctcgac gagtacgata ctgcctacgg gatgctgcga ggggttccgc tcgaactggt
       61 cgggctctgg ggttgcctgc gctttgccgc cgagggcctg ggctgcgccc tcctcccctg
      121 gctgcagcga cgcggcatcc tcaccagtct gcgctcgatg gggttctggt ccgccatcgc
      181 tgccctcatc cttgctgcgg gaatcgccgg accggatctg ctgttaccgg cctacttcgc
      241 gttttttgcc atgctggccc cggtcgaaat cgccttcggc ggacagatcc agcgccggat
      301 ccccgatacc ggtcgggcca gcttcacctc gctggtctcc ctcctgacgg ccggggcagc
      361 gatcctgatc gggctggtcc tgggtgcggc tgcctcccgg ggaggcctgc atctcctgtt
      421 tgcgaccggc gcaggcctgg tcctgctggc ttcactggcc ggcctgttcg gccggccagt
      481 caaccaagac agcacggaag gacggtgaaa cagggtacgg gcagagccgg gctgacaaag
      541 agccggagga tccggccacg aaaaggggca accactttgg caaatccctc gggggtgtga
      601 caccgggcca accaagttcg ggagtcattt cttttctttc cggaatttca gccggtgctg
      661 catgaccgca tcgaccatcc ggtccgggaa aatccgtctg aaaaaaagga tggctttggc
      721 ctcaaaagga acggcccagg agacaggcgg cctccggacc agggcagccc ggacgaccac
      781 gcgcgcaacc ttgtggacac tcgttccacc cttgccaaaa aaatccttca ttgcggagac
      841 aagccgtgca taggcgcccc gacccgaata gcgttccatg ctctcgagcg caatccggct
      901 ccagtcggtc ttgaccaggc cgggcttgac catgaccaca cggatcccga aggggcgagt
      961 ctccatccgc agggcatccg tgattgccac aagcgcatgc ttgctggcgt gataccaggc
     1021 gcccccgggg acagaaacat gcccggcaat ggatgcaata ttcacaatcc ttccgctccc
     1081 gcgttcacgc ataccgggca ggacaagctg tgtcaacctg gccaatccaa agacgttgac
     1141 ctcaaactgc ctgcgggctt cgtcaagggg aacatcctcg accgttccgt agctgccata
     1201 gcccgcatta ttgatgagga catcgatccg gccatcacgc ttgaggatgg ttgcgagtgc
     1261 cttttttacg gaggactcgt tttcaacatc cagggcaacg gcacgagccc cgacactgac
     1321 cagatcggca accttcttcg gattgcgggc cgaggcccag accgcatgcc cggcctcaag
     1381 caaaagccgg acggtctccc ggccgatacc ggccgaggcc ccggtgacga gaacgaccag
     1441 ttgtttttgc acatctttcg ccatttcgtc ctccgcggca ttcagactca cacccgtccc
     1501 gtgaccggtt gtgagcactg ttcaccagac attatttttt ccatgaatcc ttgagcctga
     1561 cagcaaggtt gaagacaggt ttttccggcc ggctgtccgg atcggctgca aaatatccct
     1621 gtcgtaaaaa ttgatattga cttcccgcgg gagcctccgc cagcgagggt tcgcccttgg
     1681 cctggcggat cactccgagc gaatcgggat tgaggttggt cttgtaatcc acgccatctt
     1741 ccacggactc cggtttttcg acggcaaaga gccggtcata caggcggatc tcgagatcga
     1801 ctgactcgct gcaggagacc cagtgcaggg tccccatcac cttgcgcccg tcatcggtcc
     1861 agcccccgcg actggcggga tcgtgggtac agcgcacctc ggcaattcgg ccgtcggcat
     1921 ccttgacgag gtcggtgcag gtcacgtaat acccgtgttt gagacggacc tcgcggccgg
     1981 gcgcaagcct gaaatacttg ggtggcggat tttccatcac atcgtcgcgt tcgatccaga
     2041 gctcgcccga aaagggaacc atccgttttc ccgcggcttc gtcctcggga ttgttgtcgg
     2101 cctccatcat ctcgaccttg ccggccggcc agttggtgat gacgagcttg accggatcga
     2161 gaacggccat ccggcgcagg gcccgcttgt tgagatcctc gcgcaggcag tgttccagga
     2221 gggcaatatc gaccgtactg tccatcttgt tgaccccgat ccggtcacaa aaatccttga
     2281 tcgattcggg tgtatagccg cgccgtctga gaccgcagag ggtcggcatg cgcggatcgt
     2341 cccaacccgc cacggacccg cccttgacga gttcgaggag cttgcgcttg gacatgacgg
     2401 tataggtcag gttgagtcgg gcaaactcga tctggcgcgg gtggaaaatc ccgagagcct
     2461 cgcagaacca gtcatagagc ggccggtgga tctcgtattc gagcgaacag agggagtgtg
     2521 tgatcccctc aatcgagtca ctctgcccgt gggcaaaatc gtacatcgga tagatcttcc
     2581 aggtatcgcc ggtccggtga tggggtgcat ggaggatccg gtacatgacc gggtcgcgca
     2641 gattgatgtt gggcgaagcc atgtcgatct tggcgcgcag ggtgcgtgat ccgtccggaa
     2701 actcgccggc cttcatccgt gcaaaaaggt cgagattctc gtcaacgctc cgttcgcgcc
     2761 agggactggg ccggccgggg accgtcaggg tcccgcggta ctcgcggatc tggtcaggcg
     2821 tcagatcgca gacgtaggcc ttgccggccc tgatgagttg gaccgcatac tcgcagagct
     2881 ggccgaaata atcagacgca taaaagagcc ggtcctccca gtcaaaaccg agccaatgga
     2941 tgtcctccat gatggcatcc acgtattccg tgtcttcctt gaccggattg gtatcgtcaa
     3001 agcgcagatt gcacttcccg ccgaattcgc gggccatccc gaaatcgatg cagagggcct
     3061 tggcatggcc gatatgcaag tagccattgg gttcaggggg gaatcgggtg tggacgcctg
     3121 aaaagcgccc gctggccaga tcctcggcca ccgccttgcg gataaaatca actgcctcgg
     3181 gggtctcacc gcccgcgttt gtaccggtct cgtttgccac agccatccct caccctgctc
     3241 agtgtttgca gtccaaaaag tacgaaattc atgggggaaa tgggagcggg acacccatcg
     3301 tcaacgattc tggtggattc gcgccaccgc tgccgccatc tcggcgggtc gctgtgtccc
     3361 gatcatgatc cgtcggccat cgtcccgcag gatctcgagc ccctgcctgc ctgagacgga
     3421 ataggcccgc ttcttccaac cacgcctgag cccccatcca ccgtattccc gtaccggatg
     3481 atagtcccgg acctcgaccc gatccgtttc tcccagaggg atgaacctcg gcttgagatg
     3541 gaagggcctg tagcggagat agaccccatc aggatggacc tcggtgatca accgggcaaa
     3601 ccagatcagg gcaaaaacag aaccccagac cacaacctgg accgaagtga tcaggatgag
     3661 agtcgtattg tcaaccggct tcgagccggc aggcagacca agaccgagct gccggaccaa
     3721 ggcccagatc gcgaagccaa gggcagcaca ggccatcagg cccaaaggca agagaatcca
     3781 gggattgcgg aatttttgtt cttcatgaaa caaggctgta ccgtcaccga agctcatcgt
     3841 gtcctcctgt ttctgcggtc caatctatca catcaccgga catgattcca gtctgaccgc
     3901 acccctcagc ggatgtagcg cacgatgccg atcagatgtg cggcactccc gccca
//
LOCUS       DAOSHE010000167         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20879_length_3954_cov_3.669659, whole genome shotgun sequence.
ACCESSION   DAOSHE010000167 DAOSHE010000000
VERSION     DAOSHE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20879_length_3954_cov_3.669659"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLD82_05665"
     CDS             complement(<1..405)
                     /locus_tag="PLD82_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG64907.1"
                     /translation="MKYETGKIGRVIVVRLEDGEIIHEQIERIARDEGIRCATVSGLG
                     GIDVGSRLVVGPREPRNLPPEPQEIVLDNVYEATGNGTIAPGENGQPVLHMHLACGRM
                     NSVVAGCIRRGVRVWHVFEVVITELEGVKARRV"
     gene            complement(484..1482)
                     /locus_tag="PLD82_05670"
     CDS             complement(484..1482)
                     /locus_tag="PLD82_05670"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG64908.1"
                     /translation="MGKRVAIVGATGAVGTEMLETLEKRNYPVDELFLFASSRSVGKK
                     LTFKGNEIPVEELNAECFKGKRIDVALFSAGASRSKEFAPPAVESGAVVVDNSSAFRM
                     DEGIPLVVPEVNPEDIRTHKGIIANPNCSTIQMVVALKPIHDAATITRVEVSTYQAVS
                     GAGQAAMDECVQQVRDYLAGNPVKIEKFLHQIAFNLIPQIDVFTDNGYTKEEMKMVKE
                     TQKIMHLPNLKVTATTVRVPVLRAHSESITISTEKKLTAAQARAIFEKAPGIVVQDDP
                     AKSIYPMPFFISGSYPVYVGRIREDISQDKGLAFFCVADQLLKGAALNAVQIAELL"
     gene            1507..2682
                     /locus_tag="PLD82_05675"
     CDS             1507..2682
                     /locus_tag="PLD82_05675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG64909.1"
                     /translation="MNDGRNIALFLFRMTRIGYTRDTYRLLKTTKERAMADTTTRDYE
                     LGRRIRSLKILRNLSEFLKILDVNEGDILKSMLVKFAIEAIEADRGALLVFDPVHRQL
                     RYHVCYIYRDHRCDDADYAAGLNEIRFELSGSMAGFALREREVIVCGSTTNDPRYNPA
                     ADALLPIRAESAIYLPISVGNEPVAVLEIANGPDNRKLDEADAETLIIITNLASAAME
                     NAQLFLWAISDPLTRLYNIHYYRRTLSSELRRAERFRSHFSVVMIDLDNFKGINDTWG
                     HPMGDQVLVEFAAILGRNLRKDVDIPSRYGGDEFILLLPNTNETGAAVVVNRILKVVR
                     ETRLVTEAGEEFGFAISVGISTFPAHGTTEDRLIENADRALYTAKEGGKNCLAVYTP"
     gene            2679..3272
                     /locus_tag="PLD82_05680"
     CDS             2679..3272
                     /locus_tag="PLD82_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64910.1"
                     /translation="MTRGWASCSLLFFLLAFSTRLDGNPAARTEPPQIADSLGIIPRP
                     LAGLEGSAIRWGMELTCTRVFLPLESVSPWLEAGIRYFERLDTYPDDKKAFLLRAGVL
                     VPFGDVHLAPGLGLVSGGGQASLELTFGLWRFFGVTARPRPRLEDYPDYGTWLDADRT
                     WERARTEETPTALGFGLSLTPWLAPQAVLSFSIAVFL"
     gene            complement(3373..>3954)
                     /locus_tag="PLD82_05685"
     CDS             complement(3373..>3954)
                     /locus_tag="PLD82_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG64911.1"
                     /translation="PLISYTGSGKFRLHSVGQCVDTLEVRIDSPDPQRVIGEILVKGE
                     NVMQGYYKNPKATREVIDKAGWLHTGDLGIVDNDGFIFIRGRSKSMLLGPSGENVYPE
                     EIEARLNNMACVQESLVVERSGQLVALVYPDPEAVDAHGMDEAGLKDLMEKNRRALNA
                     ALPSAWAIARIEMFPEEFEKTPTRKIKRFIYSV"
BASE COUNT          798 a         1263 c         1064 g          829 t
ORIGIN      
        1 gacccgccgg gccttgaccc cctcaagctc cgtgatcacc acttcaaaca cgtgccagac
       61 ccgcactccc ctgcgtatgc atcccgccac aacggagttc atccgtccgc aggccaggtg
      121 catgtgcagc accggctgcc cgttctcgcc cggcgcgatc gttccgttgc cggttgcctc
      181 atagacattg tccagcacga tctcctgggg ttccggcggg aggttgcggg gctcacgcgg
      241 tccaacgacc agccggctcc cgacatcgat tccacccagt ccgctcaccg tcgcacagcg
      301 gataccctca tcacgtgcga tccgctcaat ctgttcgtgg atgatctcgc catcctcaag
      361 ccggacgacg atcacccgcc cgatcttccc tgtctcgtac ttcatctgtt cctcctgaaa
      421 aacctgacaa aaaagccccg tctcgaatgg acggggcgca tcatcagtat cggcacacca
      481 ctgtcagagc agttcggcga tctggacagc attgagcgca gcacccttga gcaactggtc
      541 ggccacgcaa aaaaacgcca gacccttgtc ctgcgagata tcctcgcgga tccggccgac
      601 gtagaccgga taactcccgc tgataaaaaa cggcatggga tagatgctct tggccggatc
      661 gtcctggaca acgatcccgg gagccttttc aaaaatggca cgagcctggg ccgctgtcag
      721 cttcttctcg gtgctgatgg tgatcgactc ggaatgggcc cgcaaaaccg gaacccggac
      781 cgttgtcgct gtcaccttga gattgggaag gtgcatgatc ttctgggtct ccttgaccat
      841 cttcatctct tccttggtgt acccgttgtc ggtaaagaca tcgatctggg gaataaggtt
      901 gaaagcgatc tgatgaagga acttttcgat cttgacagga ttcccggcca gatagtcacg
      961 aacctgctgg acacactcgt ccatcgcggc ctgcccggca ccggagacag cctgataggt
     1021 cgaaacctcg acacgggtga tggtcgccgc gtcatggatc ggtttgagcg ccacaaccat
     1081 ctggatggtc gagcagttcg gattggcgat gatccctttg tgcgtccgga tatcctcggg
     1141 attgacttcg ggcacgacca agggaatccc ctcgtccatg cggaatgcgc tcgaattgtc
     1201 gaccacaaca gcccctgact cgacagccgg tggtgcaaac tccttgctcc gggatgcccc
     1261 ggcggagaag agcgcgacat cgatccgctt gcccttgaaa cactccgcgt tcagctcttc
     1321 aacgggaatc tcgttcccct tgaacgtgag tttctttccg accgaacggg acgaagcaaa
     1381 caggaagagc tcgtcaaccg gataattcct cttttcgagt gtctcaagca tctcggtgcc
     1441 gacagcaccg gtggctccaa caatggctac tctttttccc atggcgtctc tccttggatc
     1501 gagggtatga atgatggcag aaatatagcg ctttttcttt tcaggatgac gcgaatcggc
     1561 tatactcgtg atacgtacag actcctgaaa acaacaaaag agcgggccat ggcagacacc
     1621 acaaccaggg attacgaact cgggagacgt atccgcagtc tcaagatcct gcggaatctc
     1681 tccgaatttc tcaagatcct cgatgtcaac gaaggcgaca tcctcaagag catgcttgtg
     1741 aaatttgcca tcgaggcgat cgaggccgat cgtggcgcgc tcctggtatt cgacccggtc
     1801 caccgccagt tgcgatatca tgtctgctat atctaccgcg atcatcgctg cgatgatgcg
     1861 gactacgcgg ccggcttgaa cgagatccgt tttgagctct ccggcagcat ggccgggttt
     1921 gccctccgcg aacgcgaggt catcgtctgc ggttccacca ccaatgaccc gcgctacaat
     1981 ccggccgccg atgccctgct ccccatccgg gccgagtcgg ccatctatct tccgatctcc
     2041 gtcgggaacg aaccggtcgc tgtcctcgag attgccaacg gaccggacaa ccgcaagctt
     2101 gacgaggcgg atgccgaaac cctgatcatc atcaccaacc tggcatcggc tgcgatggaa
     2161 aacgcccagc tcttcctctg ggcgatctcc gaccccctga cgcgcctcta caacatccac
     2221 tattaccggc ggactctctc gtccgaattg cgtcgtgccg aacggttccg ctcccacttt
     2281 tccgtggtga tgatcgacct ggacaatttc aaaggaatca acgacacttg ggggcatccc
     2341 atgggcgatc aggtccttgt cgaatttgcg gccatcctcg ggcggaatct gcgcaaggac
     2401 gtggatatcc cgagccgcta cggcggtgat gaattcatcc tcctcctgcc caacacaaac
     2461 gagacaggtg ccgcagtcgt cgtcaaccgg atcctcaagg tcgtccggga gaccagactg
     2521 gtcaccgaag ccggagagga attcgggttt gccatctccg tcgggatctc caccttcccc
     2581 gcgcatggca caaccgaaga ccgtctgatc gaaaacgccg accgcgctct ctatacggcc
     2641 aaagagggcg gaaaaaactg tcttgcggtc tacacaccgt gaccagagga tgggcgtcct
     2701 gctcgctcct ctttttcctg ctggccttct ccacccgtct tgacgggaac ccggctgccc
     2761 ggacggagcc cccacagatc gccgacagtc tcggcatcat tcccaggcca ctcgccgggc
     2821 ttgaaggttc ggcgatccgc tggggaatgg aactaacatg cacccgggtc ttcctcccgc
     2881 ttgagtccgt ctcaccctgg ctggaagccg gaatccgcta ttttgaacgg ctcgatacct
     2941 acccggatga caaaaaagcc ttccttcttc gggccggtgt cctcgttccc ttcggggatg
     3001 tccatctggc gcccggcctg ggtctcgtga gcgggggtgg tcaggcctcc cttgaactga
     3061 ccttcggtct ctggcgattc tttggcgtca ccgcccgccc ccgcccccgt ctcgaggact
     3121 atccggatta cgggacctgg ctggatgccg accggacatg ggaacgcgcc cgcacggagg
     3181 agacaccgac agccctcggg ttcggcctga gcctgacccc ctggctggcc ccgcaggccg
     3241 tcctctcgtt ttcgattgct gtttttttgt aggcggacca ggcacgccac aacctggtcg
     3301 gagaaaattg cgcaactggc aggtctgttt gttgatgaac agcctcaggc cggatcgagc
     3361 cggtcaacag gatcaaacac tgtagataaa gcgtttgatc ttgcgcgtcg gagttttttc
     3421 gaactcctcc gggaacatct cgatcctggc gatagcccac gccgaaggca atgccgcgtt
     3481 cagggcacgc cggttcttct ccatcagatc cttgagcccc gcctcgtcca taccgtgtgc
     3541 atccacggcc tcgggatccg ggtagacgag cgcaacgagc tgacctgagc gctccacaac
     3601 gagggattcc tggacacagg ccatattgtt aagtctcgcc tcgatctctt ccgggtagac
     3661 attctctccg gacggcccga gaagcatcga cttggagcgg ccccggatga agatgaaccc
     3721 gtcattgtcc acaataccga gatccccggt atggagccaa cccgccttgt cgatcacctc
     3781 acgggtggct ttgggatttt tgtaatagcc ctgcatgaca ttctcgccct tgacgagtat
     3841 ctcgccaatg acacgctggg ggtccggaga atcaatcctg acctcaaggg tatcgacaca
     3901 ctgcccgacc gagtggagac ggaatttacc gctcccggta tagctgatca gggg
//
LOCUS       DAOSHE010000168         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_20934_length_3946_cov_3.814958, whole genome shotgun sequence.
ACCESSION   DAOSHE010000168 DAOSHE010000000
VERSION     DAOSHE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_3946_cov_3.814958"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLD82_05690"
     CDS             complement(<1..512)
                     /locus_tag="PLD82_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG64912.1"
                     /translation="METRAFEPGSTLSLTNDGRLELFFLGVGSAFAKRHYQTNLLIIK
                     GEDHVLIDCGTKCPQALYDVGLPVTAIKNFFITHSHSDHIGGLEEVALLGRYMAKIKP
                     VMIIAEAYQHILWDMSLRGGCAHNEEQAGDILKFSDFFEVKRPRHMADIPRLTYDTEV
                     GGIGLKMMRT"
     gene            complement(656..1219)
                     /gene="folE"
                     /locus_tag="PLD82_05695"
     CDS             complement(656..1219)
                     /gene="folE"
                     /locus_tag="PLD82_05695"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOG64913.1"
                     /translation="MRIKVAEMKDAVLAMIKHIGEDPAREGLVKTPERVEKAWAYMMS
                     GYGQKLEDVINGAVFEAPGDEMVVVRDIEIYSMCEHHLLPFFGKCHIGYIPNGQVLGV
                     SKLARIADMYARRLQIQERLSHEIAAAVMDAIEPRGVAVIIEAKHLCMMMRGVEKQNS
                     EMRTSAMLGVFRHRPETRAEFMALISR"
     gene            complement(1367..2653)
                     /locus_tag="PLD82_05700"
     CDS             complement(1367..2653)
                     /locus_tag="PLD82_05700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG64914.1"
                     /translation="MNTNELRIGIASLAQDGRGVGRVDGRVCFVEGGLPGETVLARVV
                     REKPSMLEAVTIAVESASPARCQPVCPETRCGGCALRAMEYQAQLAEKRRIVEEQLAR
                     IGKIPEPKVLPMVGMEDPFGYRNRVRLVWDGACLVFRARQSHESGGRIAFCRIASPEV
                     VRLACAVEDCLREGLIRIDRECEVLVRFGVEGYVGFEGARPARVSAEAAGRLQDAGAA
                     VLDVGGQRADLMRQDPDGEYLPDVEYRVGGMTFAVKGRSFFQTNIRMAERLFRDVLDI
                     LGSDSRGTVIDLYGGVGAPGLLCAREGRTVLSVEIDPDAVRAATFNATRAGVAYQAIG
                     GDAARVLPGLLTRFPRADLVVDPPRAGLSGEVVAALVAHPPERIIMISCDPATLARDA
                     GRLTADGTYVLEYLRPYDMFPWTHHSEALAVFVRRP"
     gene            2729..3118
                     /locus_tag="PLD82_05705"
     CDS             2729..3118
                     /locus_tag="PLD82_05705"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOG64915.1"
                     /translation="MKKTLGPVCSLLLLSGLFLTGCAGSGTGRVFPPSSVRLTWRPGI
                     TEKRPQNVFISGTFNEWHMNNPAFKCAWNPLENGFSITLTLPPGRYEYKFVVDGHWIH
                     DRDARESAPDPLGGRLGVFYVKEPAAQ"
     gene            3093..>3946
                     /locus_tag="PLD82_05710"
     CDS             3093..>3946
                     /locus_tag="PLD82_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOG64916.1"
                     /translation="MSKNRPLSDYRSARRRWLIPLTILGLIAVYIPVRLVLEAVDRNL
                     GKPIRFEYVHVPEGAHAKPLPQRVYLSGRFCDWAIAEEHFRMNFDGTNRFSLKLELEP
                     GRHPYRFVLHYPPMAPGWTNSVEFVTDPEAGSSEIFDDGSRRSVHIVRTVQGLREISN
                     FVFVAILAGLFLFALLRRALVSLMYLRISLRSKLISSFLFFLVAANIFFIRLTTVQRA
                     DFARTMQIDIINTIHTMLMSEGVSFADIARRWDMQYVIRERLERFFRSSSLREDYNTY
                     ANNKIQLTR"
BASE COUNT          838 a         1338 c          994 g          776 t
ORIGIN      
        1 ttggtgcgca tcatcttgag cccgatcccg ccgacctcgg tatcataggt caaccgcggg
       61 atatccgcca tgtgcctcgg ccgcttgacc tcaaagaaat ccgaaaactt gaggatgtcg
      121 ccggcctgct cctcgttgtg ggcacacccg cctctgaggg acatatccca gaggatgtgc
      181 tggtaggcct cggcaatgat catgaccggt tttatcttgg ccatatagcg tccaaggagc
      241 gcgacttcct cgagcccgcc gatatggtcg gagtgcgaat gcgtgataaa aaaattcttg
      301 atcgccgtca ccggaagccc gacatcgtag agggcctgcg gacacttggt tccgcagtca
      361 atcaggacgt ggtcctcacc cttgataatg aggagattgg tctggtaatg ccgcttggca
      421 aaagcactcc cgactcccag aaaaaagagt tccagccggc catcattggt cagggacagt
      481 gtcgaaccag gttcaaacgc tcgtgtttcc atcacgcacc ctcattggtt tgtcgaatac
      541 aggcaccagt atggcataca caaacgcaaa ccacaagcgg ggcgcgccgg gccatgcaaa
      601 cgggacaaga ccaaaaaaag ggattcccgg gccattcccc actatcggca ttccgctatc
      661 tcgatatgag cgccataaat tctgcccgcg tttcgggacg gtgacgaaaa acaccaagca
      721 tggccgaggt ccgcatctcg ctgttctgtt tttcgacccc acgcatcatc atgcagaggt
      781 gctttgcctc gatgatcacc gccaccccgc gcggctcgat cgcatccatt accgccgcgg
      841 cgatctcgtg cgaaaggcgc tcctggatct gcagccggcg ggcatacatg tccgcaatcc
      901 tggccagctt ggagaccccg aggacctgcc cgttcgggat atacccgata tgacacttcc
      961 caaaaaacgg caacaaatga tgttcacaca ttgaatatat ttcgatatca cggacaacca
     1021 ccatctcgtc acccggagcc tcgaagacgg ccccgttgat cacatcctcg agcttttgcc
     1081 cgtaccccga catcatgtag gcccaggcct tctccacccg ctccggggtt ttgacaaggc
     1141 cctccctcgc agggtcttcg ccgatgtgct tgatcatcgc caggactgca tctttcattt
     1201 ccgccacctt gatgcgcatt gctttcttcc ctcaaaatat agtgcgcatt caggttcttt
     1261 tgcgggtgat ggcgtccgga acctcacccc ttcttccggc ttttccggcg ggagcccaag
     1321 ctgccgagcc tcagtccgca ccccgcttga cgcccggtcg cccgcttcaa ggccgacgga
     1381 caaacaccgc cagagcctcg ctatgatgcg tccatggaaa catgtcgtac ggccggagat
     1441 attccaggac ataggttccg tcggctgtca accgccctgc atcccgcgcc agcgtcgccg
     1501 ggtcacagga gatcatgata atccgttcgg gtggatgggc tacgagcgca gccaccactt
     1561 cgcccgacaa gccggcccgg ggtggatcga ccacaaggtc ggcccgcgga aagcgggtga
     1621 ggagtccggg caaaacccgg gccgcatccc ctccaatcgc ctgatacgcc accccggcac
     1681 gggttgcatt gaaggtggcc gcccggaccg cgtccggatc gatctccacc gagagaaccg
     1741 tccgtccctc tcgggcacag aggagtcccg gggcaccgac accaccatag agatcgatga
     1801 ctgttccccg tgaatcactg ccaagaatat ccaaaacatc ccggaacagg cgctccgcca
     1861 tccggatgtt cgtctgaaaa aacgagcgcc ccttgaccgc aaaggtcatg ccacccacac
     1921 gatactccac atccggaaga tattctccgt cgggatcctg ccgcatcagg tcggcccgct
     1981 gaccgcccac gtcaagaacc gccgcgcccg cgtcctgcaa ccggccagcc gcttccgcgg
     2041 aaacccgtgc cgggcgcgcg ccctcgaatc cgacataccc ctcaacgcca aagcgcacca
     2101 ggacctcgca ctcccggtcg attctgatca gcccttcccg gagacagtcc tcgaccgcac
     2161 aggccagcct gacgacctcg ggactggcaa tccggcagaa ggcgatccgc ccgccgctct
     2221 cgtgactctg tcgggcacga aagacgagac aggcaccatc ccacaccagc cgcacgcggt
     2281 tccggtatcc gaacggatcc tccatcccga ccatgggcaa aaccttgggc tccggaatct
     2341 taccgatccg agccagttgc tcctccacga tgcgcctttt ttccgcaagc tgggcctgat
     2401 actccatggc acgcagggcg catccgccac acctcgtctc gggacagacc ggctggcagc
     2461 gggccggcga agccgattca acagcaatcg tcaccgcctc gagcatgctg ggcttttccc
     2521 ggacaacccg cgccaacaca gtttctccgg gcagcccgcc ctccacaaaa caaacgcgcc
     2581 catccacacg gcccacaccc cgaccatcct gtgccagact cgcaatcccg atccgcaact
     2641 cgttcgtgtt cattccacaa aggtaccaca gagaagaacc gctgagagca tggcgacaaa
     2701 caccccgaaa actgtgtatt ttaacggtat gaaaaagaca ctcggtcctg tctgtagcct
     2761 gcttcttctt tcaggcctgt tcctcaccgg ctgtgccggg agcgggaccg gccgtgtctt
     2821 tccaccatca tccgtccgtc tcacctggcg gccaggcatc acggaaaaaa gaccgcaaaa
     2881 cgtcttcatc tccggaacct tcaatgaatg gcacatgaac aaccccgcct tcaaatgcgc
     2941 ctggaatccg cttgaaaacg gattcagcat caccctcacc ctccctccgg gaaggtatga
     3001 gtacaaattc gttgtcgatg gccactggat acatgatcgc gatgcccgcg agagcgctcc
     3061 agatccattg ggcggaagac tgggagtgtt ttatgtcaaa gaaccggccg ctcagtgact
     3121 accgcagcgc ccggcgacgc tggctcatcc ccctgaccat cctgggactg atcgctgtct
     3181 acattcctgt ccgcctggtc ctcgaggctg tcgaccgcaa tctcggcaaa ccgatccgct
     3241 tcgagtatgt ccacgtaccc gagggagcac atgccaaacc cctcccgcaa cgcgtctatc
     3301 tcagtggccg gttctgtgac tgggccatcg cagaagaaca cttccggatg aatttcgacg
     3361 ggaccaaccg cttttcactc aaactcgaac tcgaaccggg ccggcatccc tatcgttttg
     3421 tcctgcacta tccaccaatg gctcccggct ggaccaatag cgtcgagttc gtcacggatc
     3481 cggaggcggg cagcagcgag atctttgacg acggaagccg ccggtcggtc cacatcgtcc
     3541 gcacggttca aggcctgcgg gagatcagca attttgtctt cgtcgccatc ctcgccggtc
     3601 tttttctctt cgcgctcctc cggcgtgctc tcgtcagcct gatgtatctg cgcatctcgc
     3661 tccgctccaa actgatttcg agctttctct ttttccttgt tgccgccaac atctttttta
     3721 tccgcctgac gacagtccag cgcgccgatt ttgccaggac catgcagata gacatcatca
     3781 ataccatcca tacgatgctc atgagcgagg gtgtctcgtt tgccgatatt gcccgacgct
     3841 gggacatgca gtacgtcatc cgggaacgtc ttgaacggtt cttccgctcc tccagcctca
     3901 gggaagatta caatacctac gccaacaaca aaatccagct cacccg
//
LOCUS       DAOSHE010000169         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21068_length_3929_cov_4.800981, whole genome shotgun sequence.
ACCESSION   DAOSHE010000169 DAOSHE010000000
VERSION     DAOSHE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21068_length_3929_cov_4.800981"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLD82_05715"
     CDS             complement(<1..1061)
                     /locus_tag="PLD82_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOG64917.1"
                     /translation="MIARLFIVLIVVLSSGILSAMPPHPDVYNRMGETQRRDLSIRLQ
                     AQTKKGMDRPMRAFPTTGNRKIPVLVVAYSDRAMSAISTKTFYENLLNGATSSDMSMR
                     KYYQDMSNGLLNLTIDVHGPYTAPNTLAYYGANTGSGDSDAHPGELVGYAIDQAEAAG
                     VNFAQYDNDGDGAVDIVMVIHAGQGEESSSSENDVWSHAWDLASAKYYGDGTGNRVYD
                     GKTISKYTIQPEYNDAAGDSTIGVFCHEFGHVLGLPDMYDTSYETYGAGKFTLMAGGS
                     WLGATSGSRPAPLLAWEKRKLGWITTKLPDGTTVADASGVQDVMLASRTSAGQSRPLP
                     LVPILALGILGLGGFFSWRR"
     gene            1587..3533
                     /locus_tag="PLD82_05720"
     CDS             1587..3533
                     /locus_tag="PLD82_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64918.1"
                     /translation="MQPLTVTLRGWRGTVIPLLAGFLALAGASASFDISAPPLTVSVV
                     WSMLFPFIVAFAYGTRGAFLASLPTAAIPFLLWSNNGWACLPASLIYMTIIVGTGWLS
                     GLRRKKSPLISLLTFTLPAMALLEILFFPPMMLLNPPGWAEHALTSIDHSILVGIAVK
                     DMLTLFIYSLTAAHLVRIPFVRRLLGLNPLKEYRAAGGIFAVSFVTGFAILSVYTILA
                     DILVHQSLTIHAFPDLSKPPIGLAFLVNLAASLLAGLHISDFLARQRASEQELAATEK
                     RKSAILAAIPDLLFILSRDGRFVDYVSSQPEKLLIRPDHFIGQSIHDVLPPSIAIPTA
                     NAMEHVDEGEQRFTYTLDDGSVSRDYEARVIAWSTSEILIIIRDMTEDRHREHQIARL
                     EAQLLQEQKLSAIGTLSGSVAHEINNPLMGIINLAQLILDNSEKARSNDPEIASLLGR
                     VKEFSCEIISEGERIASIVRALLSVARQEESGMVPIQPVELIESVHSLVSKLFAKGDI
                     TMDVIIDSDAPAVFCNPAQIRQILLNLLTNARDAIEASDRHSPATSRITLHAQRHTDD
                     QTWLRISITDTGDGIDASIVQQIYDPFFSTKARGKGTGLGLSVSKEIIEKHHGRISCE
                     TIQDEGTTFHVDLLAGPNTAPLQA"
     gene            complement(3524..>3929)
                     /locus_tag="PLD82_05725"
     CDS             complement(3524..>3929)
                     /locus_tag="PLD82_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG64919.1"
                     /translation="REDFTRIPNGVSGIEERLKLLWTFGVARGRITPQRFVELTSTRA
                     ARIFGMYPQKGSLAVGSDADILVWDPAQRTRFGASTQVSRCDESIWEGFEGVGAPRFV
                     FSRGTEAVREGQVMAEAGHGHYLERRLLEGHA"
BASE COUNT          853 a         1229 c          985 g          862 t
ORIGIN      
        1 cggcgccatg agaagaaccc gcccagaccg aggataccga gtgcgagtat tggcacaagc
       61 gggagcggcc ggctttgccc ggcagaggtg cgagatgcaa gcatcacatc ctgcacaccg
      121 ctggcatccg cgaccgtcgt tccatcgggc aacttggtcg tgatccagcc aagcttgcgt
      181 ttttcccagg ctaaaagcgg agccggtcgt gatccggagg ttgcacccag ccaggaacca
      241 cccgccatca gggtaaactt gccggccccg taagtttcgt aggatgtatc gtacatgtcg
      301 ggtaggccca ggacgtgtcc gaactcgtga cagaacacgc cgatcgtcga atcgccggcc
      361 gcatcgttgt actcgggctg gatcgtgtac ttgctgatcg tcttgccgtc atacacacgg
      421 ttacctgttc cgtcaccata gtacttggca ctggccagat cccaggcatg cgaccagaca
      481 tcgttttccg agctggaact ctcttcgccc tggccggcat ggatgaccat cacgatatcg
      541 acggccccgt cgccatcatt gtcatactgg gcaaaattga caccggccgc ctcggcctgg
      601 tcgatcgcat acccgacaag ctcacccgga tgggcgtcac tgtcaccgga accggtattg
      661 gcgccgtaat acgccagcgt attgggtgcc gtgtacggtc catgcacgtc aatggtcagg
      721 ttgagcagcc cgttggacat gtcctggtag tacttgcgca tcgacatgtc ggaggatgtc
      781 gccccattga ggaggttttc gtaaaacgtc tttgtcgaga tggcactcat ggcgcggtca
      841 ctgtaggcca cgacgaggac agggattttc cggttgcccg tggtgggaaa ggccctcatc
      901 ggccggtcca tccccttttt ggtctgggcc tgcaggcgga tcgagagatc ccgacgctga
      961 gtctcgccca tacggttata aacatcggga tggggcggca tggcagagag tattccagac
     1021 gacaggacaa cgataagcac tatgaacaga cgagctatca tacaaaggac tcctttgcga
     1081 agagacgtat tcacgggacg atggttcttc tattataaac caaattctgt atttgagaac
     1141 tcaaaaaatc tcttcaagaa aatccgtgac ccgaagggag aacctgcagg gttatgcgcg
     1201 agtatcagct aatcccggcg aagatccctc aaggatattg ccccaattgc ctgaattgta
     1261 cgcactgttt tttcagaaat tccaagaggg ctcggtcaaa tatccctgaa tttactggct
     1321 tattacaaaa tcgtcgtggt ttaagcgtga ccatcaggta atcccggatt actctattgc
     1381 gtaaatttca agcaccgtac ctgcctccgg cggatctatc ccggaaaccc atgacagtat
     1441 cgtgaacctt tgatctgacg ctcccggcca gggaccgact cgcccaaaag gttccaaaaa
     1501 ccagtctctc ggcatcttcc attactgttg tagaattgcg tccggaatga taaactctag
     1561 gagttttgga ggattccttg tcacagatgc aaccactcac agtcaccctc cggggctggc
     1621 gtggtacagt catcccgctt ttggccggtt tccttgcact cgcaggggct tcggcctcct
     1681 ttgacatcag tgccccaccc ctcacggttt cggttgtctg gtcaatgctc ttccccttca
     1741 tcgtcgcctt tgcctacggc acccgggggg cttttctggc ctccctcccg acagccgcca
     1801 tccccttcct cctctggtcg aataatggct gggcctgtct cccggcttca cttatctaca
     1861 tgacgatcat cgttggaaca ggctggctct cagggcttcg taggaaaaaa tcgcccctca
     1921 tcagtctgct cacctttaca ctcccggcca tggcgctcct cgagatcctc ttctttccgc
     1981 caatgatgct cctcaacccg ccaggctggg ccgaacacgc actgacctcc attgatcact
     2041 caatccttgt cggaattgcc gtcaaggata tgttgacctt gttcatctac agcctgacgg
     2101 ctgcgcacct tgtacgaata ccctttgtcc ggcgcctgct cggcctgaat ccccttaagg
     2161 aataccgagc tgccggcgga atttttgccg tatccttcgt aaccggattt gccatcctct
     2221 cggtctacac cattctcgca gatattcttg tacaccaatc cctgaccatc catgcctttc
     2281 ctgatctgag caagccgccg atcggtcttg catttttggt caacctggca gcctcgctct
     2341 tggccggcct gcacatcagc gatttcctcg cccgccaacg ggcaagtgaa caggaattgg
     2401 ccgctaccga aaaacgcaag tccgccatcc tggccgccat cccggacctg cttttcatcc
     2461 tctcgcggga cggacgcttt gtggattatg tctcctccca acccgaaaaa ctcctgattc
     2521 ggccggacca ctttatcgga cagagtatcc acgatgtcct ccctccttcg atcgccatcc
     2581 cgaccgccaa tgccatggaa cacgtcgatg agggcgaaca gcgttttacc tacacactgg
     2641 acgacggctc tgtttcccgt gactacgaag cgcgagtgat cgcctggagt accagtgaga
     2701 tcctgatcat catccgcgac atgacagaag accgtcaccg cgagcaccag atcgcccggc
     2761 tcgaggcgca acttctccag gaacaaaaac tctcggccat cggaaccttg tcgggaagcg
     2821 tggcgcacga gatcaacaac cccttgatgg gaatcatcaa cctggcgcag ctgattctcg
     2881 acaacagcga aaaagcccgc agcaacgacc cggaaatcgc gtcgctgctt ggacgtgtca
     2941 aggagttttc ctgcgaaatc atctccgaag gggaacgcat cgcttccatt gtgcgtgccc
     3001 tgctctccgt cgcccgtcag gaagaatcgg gaatggtgcc catacaaccc gtcgaactga
     3061 tcgagagcgt ccactccctg gtctcgaaac tctttgccaa aggtgacatc acgatggacg
     3121 tgatcattga cagtgatgcc ccggccgttt tctgcaaccc ggcccagatc cgccagatcc
     3181 tcttgaatct cctgaccaat gccagggatg ccatcgaggc atcggacaga cactccccgg
     3241 ccaccagccg gatcaccttg cacgctcagc gccataccga tgatcaaaca tggctgcgca
     3301 tctcgataac ggataccggt gacggtattg acgcctccat agtgcaacag atctacgacc
     3361 ccttcttttc aaccaaagcg cgaggaaagg gaaccggact gggcctctca gtcagcaagg
     3421 aaatcatcga aaaacaccat ggacgaatct cctgcgaaac catccaggac gagggcacga
     3481 ccttccatgt cgatctttta gccggtccaa acactgcccc gcttcaggca tgaccctcga
     3541 ggagcctgcg ttccagataa tgcccatggc ccgcctcggc catgacctgt ccttcccgga
     3601 cggcttctgt tccccgtgaa aaaacaaagc gtggtgcccc gactccctcg aatccttccc
     3661 agatcgattc gtcgcaccgt gagacctgtg tcgaagctcc aaaccgggtc cgttgcgccg
     3721 gatcccagac aagaatgtcc gcatccgacc cgaccgcaag actgcctttt tgtggataca
     3781 ttccgaaaat cctggcggca cgcgtgctgg tcagttcgac aaaacgctgg ggcgtgatcc
     3841 tcccgcgagc cacaccaaac gtccagagaa gtttgagccg ctcttcgatt ccggagaccc
     3901 cgttgggaat ccgggtaaag tcctcccgc
//
LOCUS       DAOSHE010000170         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21279_length_3901_cov_4.451638, whole genome shotgun sequence.
ACCESSION   DAOSHE010000170 DAOSHE010000000
VERSION     DAOSHE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21279_length_3901_cov_4.451638"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLD82_05730"
     CDS             complement(<1..497)
                     /locus_tag="PLD82_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG64920.1"
                     /translation="MAQERTFHHGGMHPRDIGLDTMSSPIRNAFLPHFLYIPLLQHIG
                     SPAEAVVSPGDIVSRGDVIGKASGYVSVDIHTPLPGRVVAIEDHVTATADKVPTVVIE
                     VEGAFSLKGQRQENQQGWKSLSRDEILKRMKEKGLVGLGGATFPMHVKYAVPPGKKVT
                     TLIVNA"
     gene            651..2090
                     /locus_tag="PLD82_05735"
     CDS             651..2090
                     /locus_tag="PLD82_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64921.1"
                     /translation="MKKPGYCVFGLVMALHACQGPVATTIAPPSGNIESATNCTLTLA
                     TPLGNTNLPMARMTDGWFETTFWVTTGLRILLVPSVNNGSRFLLWSGHGQSLTPWPTI
                     TGGPRPFPLAFTATGDAVCTVRINPETLACTIQPETLVANLNTRSTFSLVSDSALPDS
                     LDPALPHTRMNRIDGSTWQIILTNLIPGRPVQFAFSASMAPQELLLASSGNSGPFILD
                     AHIPGQNSIWLRHIPLSEEAVITLSPAVPSWSLSANGKPLFGDGYDDPKYLAWRMSAT
                     NSAWGLRSVAARVTLQAWYFLVDASFSSGKATLDGKQGYACVLVFDDPALSSGYTDSL
                     PSINGLPGISLPTGISVEYMLIMQATDPQSDLEGVPAVSTVLYGPLLAGLPFPSSGHI
                     LGALRTDTPDGYLAWGNRYEIVLPKKRSDGTPLLPSTALRISAFAWNAGTAWGDLSNI
                     SLQAPIAEAIPWGSSNSSLGEASTWLTVP"
     gene            complement(2095..2556)
                     /locus_tag="PLD82_05740"
     CDS             complement(2095..2556)
                     /locus_tag="PLD82_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG64922.1"
                     /translation="MEITLFCVGKLDKVWAPLANEYIKRIEYFSKIKVIEIPEPAGRY
                     RNSDESVAVTSRQLAEKIGPAPWILLDRTGASCTSEEFSKMMMRELENHPGRLNLVIG
                     ASDGVSPDLQQGAKHRISFSALTFPHQLFRVILLEQIYRAFAIARNFPYHK"
     gene            complement(2825..>3901)
                     /locus_tag="PLD82_05745"
     CDS             complement(2825..>3901)
                     /locus_tag="PLD82_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOG64923.1"
                     /translation="IVWLGKPEIGSKKNLPLVTGSLDLAWADGQVETIIDDEGYSSWV
                     ALDDVPVGLALAVVGVEDFRYFRHPGLDINQIYYAIIENIRNLGFTYGASTITQQLVK
                     NVWLANTRSFTRKLAEAIMALELEGKLSKQEILEWYLNMCEMAPGIYGIRAGSRFYFG
                     LEPHELRYGQQVFLAAVIHAPAWYAGHPERAIPRIRQLAVNLVEQKRITPSQASALSN
                     VPFEFAKMRPKPNDTGLRKALLRLWPASSRTGKNLVLQSRLDGAVQAELEQLMHDLRA
                     DGGDVFVVDGGGRSLVLASRDGAERMRSIDPELRTLSFKDGLQALPSVIISRIRTNSI
                     AFSTNHVFRMPVDWSLLPGYKHRL"
BASE COUNT          863 a         1121 c         1007 g          910 t
ORIGIN      
        1 gcattcacaa tcagagttgt gacttttttc ccgggcggga ctgcgtattt gacgtgcatg
       61 ggaaacgtgg ccccacccag accaacgagg cctttctctt tcatccgttt gagaatctcg
      121 tcgcgggaca gcgacttcca gccctgctgg ttttcctgtc gctggccctt gagggaaaaa
      181 gcgccttcga cttcaatcac gaccgtcgga accttgtcgg ccgtcgctgt cacatgatct
      241 tcgatggcga caactcttcc cggcagggga gtatgaatgt cgacggacac gtatccggac
      301 gctttcccga tcacatcgcc gcgactgacg atatcgccgg gagaaacaac cgcctcggct
      361 gggctcccaa tatgttgaag gagtggaata tacaggaaat gcggcagaaa tgcgttccgt
      421 atcggagagg acatggtgtc cagtccgata tcacggggat gcatgccacc atgatgaaac
      481 gtccgttctt gagccatact gacccctgat tgttgtggcg ttgagcaggt atgcccgaaa
      541 agccggcatc acggatacaa agaatcctgc tcaagtaatc taacttccct tctttggtcc
      601 cggcaaggct ggccttgatt tattccggag tcagggatag actcagcggt atgaagaaac
      661 ccggatattg tgtctttggc ttggtcatgg cgctccatgc atgtcaggga cctgttgcga
      721 cgaccattgc accaccgtcc ggaaacatcg agtctgcaac aaattgcacc ctgactctcg
      781 ccacgccatt gggaaacacg aacctgccca tggcccgaat gacggatggc tggttcgaga
      841 ccacgttttg ggtgacaacc ggtttacgga tccttctcgt cccgtcggtc aacaatggtt
      901 cacgcttcct gctctggtcc ggccatgggc agtcgctgac tccatggccg acaatcaccg
      961 gggggccgcg cccatttccg ttggccttca cagccacggg cgatgccgtc tgtaccgtga
     1021 gaatcaaccc ggagacactc gcctgcacca ttcagcctga aaccttggtt gcaaacctca
     1081 atacacggag cactttcagc ctggtctcgg attcggcact gcccgactct cttgacccgg
     1141 cactccccca caccaggatg aatcggatcg acggcagtac atggcagatc atcctcacca
     1201 acctcatccc gggtcgcccc gtccaattcg cattttctgc aagtatggct ccgcaggaac
     1261 tccttctcgc ttcctcggga aactccggac cattcatcct cgatgcgcac atacccgggc
     1321 agaattccat ctggctgcgc cacatcccct tgtcggaaga agcggtcatc acgctttctc
     1381 ctgccgtccc ctcctggagt ctttcggcca atggcaaacc actctttggc gacgggtacg
     1441 atgacccgaa atacctcgca tggagaatga gtgcaaccaa cagtgcatgg ggactgcgtt
     1501 ccgtggcagc cagagtcacc ctccaggctt ggtattttct tgtcgatgcg agtttttctt
     1561 ctgggaaagc gactcttgac gggaaacagg ggtatgcctg tgtcctcgtt tttgatgatc
     1621 cggctctctc cagcggatat actgactcac ttccgtccat caacggactg cccggaatct
     1681 cgcttccaac cggaatctcc gtcgaataca tgttgatcat gcaagcaacc gaccctcagt
     1741 ccgaccttga gggtgtcccg gctgtttcta ctgtcttgta cggtcccctc ctggcgggtc
     1801 tgccatttcc gtcaagcggg catatcctcg gagcactgcg gacggataca cccgacgggt
     1861 atctcgcctg gggcaaccgc tatgaaattg ttcttcccaa aaaacggtct gacggcaccc
     1921 cccttctccc gtccacagca ctccgtattt ctgcatttgc ctggaatgcc gggacagcct
     1981 ggggggatct ttcgaacatc agcctccagg caccaatcgc cgaagcgatt ccatggggat
     2041 cgtccaattc aagccttgga gaagcatcaa cctggttgac ggttccctga tcggctattt
     2101 gtggtagggg aaatttcggg ctatggcaaa agccctgtag atctgctcca gaagaatcac
     2161 ccgaaagagt tgatggggaa aggtcaaggc tgaaaaggat atcctgtgtt ttgcaccctg
     2221 ttgcaaatca ggggagacac catcggaagc accgatcaca agattcaacc gtccgggatg
     2281 gttctcgagt tcacgcatca tcatcttcga gaattcctca ctggtacagg atgcacccgt
     2341 tctgtcgagg agtatccagg gggccggtcc aattttttcg gcaagttgcc tgcttgtgac
     2401 ggccactgat tcgtcactgt tgcggtatct tccggccggc tccggaattt cgatgacctt
     2461 gatttttgaa aaatattcga tcctcttgat gtattcattc gcaagaggtg cccagacctt
     2521 gtcgagtttt ccgacacaaa acagcgtgat ttccataccc ataaaaaaaa cagggccaca
     2581 cccgtcgggc aagccctgtt ttttgggaca atgagtcctg ggtattactc tgctcccgaa
     2641 gtaacatcgg cctttgtgcc tgagccggcg caaccggcaa cgttgatggc catgacgacc
     2701 gaaaggacga gcagagcaat aacgacgact ttcttgaaca tgacttcctc ctgattggtg
     2761 aacttcaccc attcagtgta gcccgttttg gaaaaaactc aagttttttt caaaaccgcg
     2821 aggattacaa tcggtgcttg taccccggga gaagcgacca gtcaacaggc attctgaaca
     2881 cgtgattcgt cgaaaaagca atggaatttg tccgtatcct ggagatgatc acggaaggca
     2941 gggcctgaag accgtccttg aatgaaagag tcctcagttc cggatcaatg gaccgcattc
     3001 gttcggcacc atccctgctc gccaggacca gggatcggcc tccaccgtcg accacgaaca
     3061 catcacctcc gtcagccctg aggtcatgca tcaattgttc gagttccgcc tgtactgccc
     3121 catccagtct cgactggaga accagattct ttccggttct ggaagatgcc ggccagagtc
     3181 gcaagagcgc ttttcgcaag ccggtatcat tgggctttgg tctcattttg gcgaattcaa
     3241 acgggacatt gctgagggcc gatgcctggg acggcgtgat ccgtttttgt tcgacaagat
     3301 tgaccgcaag ctgacggatc cggggaatgg cccgttcggg atgaccggca taccaggccg
     3361 gagcatggat cacggcagcc agaaagacct gttgcccata gcggagttca tgcggttcga
     3421 gaccgaaata gaatcggctc ccggcgcgga ttccgtatat tccgggggcc atttcgcaca
     3481 tattgagata ccattcgagg atctcctgct tgctcaattt cccttcgagc tcgagagcca
     3541 tgatggcctc tgccaacttt ctggtaaaac tccgggtgtt ggccagccag acatttttga
     3601 caagctgttg cgtaatcgta ctggccccgt acgtgaatcc caggttccgg atgttttcga
     3661 tgatggcata atagatctgg ttgatgtcca gcccggggtg acggaaatag cggaaatcct
     3721 cgacaccgac tacggccagg gccagcccga caggaacatc atccagggca acccatgaag
     3781 aataaccctc atcatcgatg atggtttcca cctgaccatc tgcccaggca aggtcgagcg
     3841 accctgtaac cagaggaaga ttttttttgg atccaatctc gggtttgccc agccagacaa
     3901 t
//
LOCUS       DAOSHE010000171         3892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21357_length_3892_cov_3.004431, whole genome shotgun sequence.
ACCESSION   DAOSHE010000171 DAOSHE010000000
VERSION     DAOSHE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21357_length_3892_cov_3.004431"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..3451)
                     /locus_tag="PLD82_05750"
     CDS             complement(380..3451)
                     /locus_tag="PLD82_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG64924.1"
                     /translation="MDHADLQSRIEEISGAIVLVEPGDGKALNSLHGMLREVAQASEA
                     AGFSTLAGDARKAGKIIEKLVFDAPANAGEMLAQAGTIVSSMQSAWRDANAPAGRKPA
                     GSSADDSRDGSVAGPGQDVQDGETPEDGALEEETPGRQPDEEPESAGRPVELDQKSGD
                     TLTGEDFTSELTDISIVDSPSVDDDLVIEDDVTEDIDPDSFLSPGLDEEIVFSSLAGS
                     DFIESQPAQGELASPEGSPVGETPVESGESTPDSGPVVEPVASGVESGRPGFLPENAD
                     MEIFTEFLSRQPGVLEEMEEYILALDRTRDEGSINKLKRLIHTMKGESGLLGLNEVQK
                     VCHATESLLEEGSSHAHLVDILLAVKDWLTRAFAAYAGNGIEPGPAAVLIELLFETDW
                     VDPEEEMRGEVILQESPDAENGSGSELPVAEAETVRPEGGVLLQDGDAELLNDFINEA
                     SEHLESVESHMLTLESTPEDETSLNAVFRAFHTIKGVAGYLALDQIGYLAHDVENLLD
                     RARKGDLLLKGKSIDALFEAVDMMKQLVANVRDAMANGTPLVHEPGLNSIVEMINQVA
                     RGGKYVPSKPAAPEAPAPAVETKPQAARVQQAQPAPEVVHNEKDHDDGEAQQRTAGDG
                     QFVQVRESIKVDADRLDRLIDTIGELVITESMVSQMMRDKYSDAHMMSNLSQLDKITR
                     QLQEMSTSLRMVPVRATFQKMARLVRDLAKKSGKRIDFIMTGEDTELDKSVVDKIGDP
                     LVHMIRNAVDHGIESGLDRVKSGKPEVGRVELRAFHKGGSIFIEIADDGKGLVREKIL
                     AKAIENRLISEGSTLSEKEIFSLIFKPGFSTATVVTDVSGRGIGMDVVRRNIESLRGR
                     IDIQSEPGRGSIFSIRLPLTLAIIDGMMVGVGDERYIIPTLSIVRSLRPEKSDFSSVQ
                     GRGEMYSLDGVMIPVFYLNRLFGVENSRESVEDGLLVVVENDGLYTGLVVDELFGQHQ
                     IVIKTLGRLLQGIPGISGSTIMTDGHVALILDIAGLVKLANREQISAGVQG"
     gene            complement(3555..>3892)
                     /locus_tag="PLD82_05755"
     CDS             complement(3555..>3892)
                     /locus_tag="PLD82_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64925.1"
                     /translation="PDGMILASGGSDSCVRIWHEVNGIWREKTTLRGYAEGVFSLVFD
                     SQGRYLATGSKDHWVKIWERVDEDFSLENTLSGHTQCVFSLAFEPHGLMLATESSDRQ
                     IKIWPVRQG"
BASE COUNT          804 a         1269 c          980 g          839 t
ORIGIN      
        1 gggcggtttt tttatttcga cacagccccc cccaaaaaaa aaggactttc tcggtgtttt
       61 tcaaagaata cgattcgcaa ctggctcact ctccgattct tcgtgagcat aggtattcac
      121 tgcagcgtgc tgctcaccct cttcgcgcag gcccagagac cccgtgggta tcctgcgggt
      181 agccgtggcc gtctatcggg tgtatgcgca cagccacacc acgaaagatc cgtcccggtc
      241 acacggagcc ctcattccgg aagacgggca gaagtccgcc cagacaaaca caccgggagc
      301 gcttccagcc catgtcatgc ctgaggctgt tcctcataaa aaaccattcc tcaaaccgga
      361 aaacacaccc ggctggaacc tacccttgca cacctgccga gatctgctcc ctgttcgcca
      421 gcttcacaag tccggctatg tccagaatca gtgccacatg gccatccgtc atgatcgtcg
      481 acccggatat cccgggaatc ccctgcaaca aacgcccgag tgtcttgatg acaatctggt
      541 gctgaccaaa gagttcatcc acgaccaacc cggtatagag tccgtcgttt tcgaccacga
      601 ccagaaggcc atcctcgaca ctctccctgg aattctccac cccgaagagc cggttgagat
      661 agaaaaccgg gatcatcaca ccgtcgagtg aatacatctc tccccgcccc tgtaccgagg
      721 aaaagtccga tttttccggg cgcaaggagc ggacaatcga aagcgtcggg atgatgtacc
      781 gttcatctcc gaccccgacc atcattccat cgatgatcgc cagcgtcagc ggcagccgga
      841 tcgagaaaat gctcccccgg cccggttcac tctggatgtc aatccgtccc cgcagggatt
      901 cgatattccg gcgcacgaca tccatcccaa tcccgcgccc cgagacgtct gtcaccactg
      961 tcgccgtcga gaatcccggc ttgaagatca gactgaagat ctccttttcg ctgagtgtac
     1021 ttccttccga aatcagcctg ttctcgatcg ccttggccag gatcttctcc cggacaaggc
     1081 ccttcccgtc atcggcaatc tcgataaaaa tactcccgcc cttgtggaag gcccgcagct
     1141 cgacccggcc gacctcgggc tttccggact tgacacgatc cagcccactc tcgattccgt
     1201 gatccacggc gttgcggatc atgtgcacca ggggatctcc gatcttgtcg acaaccgact
     1261 tgtcgagttc tgtatcctcc cccgtcatga tgaagtcgat gcgcttgccc gactttttcg
     1321 cgagatcgcg cacaagccgg gccatcttct ggaaggtcgc ccggacggga accatccgca
     1381 gcgaggtcga catctcctgc aattgtctgg tgatcttgtc cagctgcgag aggttggaca
     1441 tcatgtgggc atcgctgtac ttgtcccgca tcatctggct gaccatcgac tcggtgatca
     1501 cgagctcacc gatcgtatcg atcagcctgt ccagccggtc ggcatccacc ttgatggatt
     1561 cacgaacctg gacaaactgg ccatcgccgg ccgtgcgctg ttgcgcttcg ccatcatcat
     1621 ggtctttttc attgtgaaca acctcaggcg caggctgggc ttgctgcaca cgcgccgcct
     1681 ggggtttcgt ttcgacagca ggggcaggcg cctcgggggc tgccggtttc gacggaacgt
     1741 atttcccgcc acgggccacc tgattgatca tttcgacgat cgaattcaag cccggttcat
     1801 gtaccagagg tgtcccgttc gccatcgcat cacgcacatt ggctacaagc tgcttcatca
     1861 tatccacggc ttcaaagagg gcatcgatcg attttccctt gagcaggaga tcccctttgc
     1921 gcgcacggtc caggagattc tccacatcat gggcgagata cccgatctgg tccagcgcaa
     1981 ggtatccggc tacccctttg atcgtgtgga aagccctgaa gacggcattg agcgatgttt
     2041 catcctcggg agtactctcc agcgtcaaca tatggctctc cacgctctca agatgttcgg
     2101 aagcctcatt gatgaagtca ttcaggagct cggcatcacc atcctgcaac aggaccccgc
     2161 cctcgggtct gacggtttcc gcttcggcca cgggaagctc acttccggat ccgttctctg
     2221 cgtccggaga ttcctgcagg atcacctcac cccgcatctc ctcttccgga tcaacccagt
     2281 cggtttcaaa aagaagctcg atcaggacag cagccggacc cggttcgatt ccattgccgg
     2341 cataggcggc aaacgctctc gtgagccagt ccttgactgc caggaggatg tcaaccagat
     2401 gggcatggga ggatccttct tcgagaagac tctcggtcgc atggcagacc ttctgcacct
     2461 cattcaaccc gagaaggccg gattccccct tcatggtgtg tatcagccgc ttaagcttgt
     2521 tgatacttcc ctcatcccgg gttctgtcga gggcgaggat atactcctcc atctcctcga
     2581 gaacacccgg ctgacgggac agaaattccg taaagatttc catatcggcg ttttcgggaa
     2641 gaaagccggg gcggccggac tcaacaccgg atgcaactgg ctccacgacc ggcccggaat
     2701 ccggagtact ctctcctgac tccaccggtg tctccccgac cggcgaaccc tccggagaag
     2761 ccaattcacc ttgcgccggt tggctctcaa tgaaatcgct tccggccagc gaggaaaaca
     2821 caatctcctc atcgagcccg ggagacagga aagaatcagg atctatatct tcggtcacat
     2881 cgtcttcgat cacgagatca tcatccacgg aggggctgtc cacgatactg atatcggtga
     2941 gttcactggt gaaatcctca cccgtcaggg tatccccgga tttttgatcc agctcaaccg
     3001 gcctgccagc ggattccggc tcttcatccg gttgccggcc cggagtttct tcttcaagcg
     3061 caccatcttc gggagtctcc ccgtcctgca cgtcttgacc ggggcctgca acacttccgt
     3121 cacggctgtc atccgcagag gacccggccg gtttgcgacc ggccggagca tttgcatcgc
     3181 gccaggcaga ctgcatgctg gagacgattg tcccggcttg cgcaagcatc tcccccgcat
     3241 ttgccggagc atcaaaaacc agtttctcga taatcttgcc ggccttgcgt gcatccccgg
     3301 caagcgtcga aaagccggcc gcctcggatg cctgagccac ctcacgcaac atcccgtgca
     3361 ggctgttgag ggccttgccg tcacccggtt ccacgaggac gattgccccc gagatttctt
     3421 ctatcctgct ttgcaagtct gcatgatcca tgggcaccaa tcctctgatg gttatcatcc
     3481 ccgtcccgga tcatccccgg tacaggtagt cctcagttca ttgtcggcag gcacgcccct
     3541 ggaaaacagg gccatcaccc ctggcgaacc ggccagattt tgatctgcct gtcactgctc
     3601 tccgtcgcaa gcatcagccc gtgcggctca aaagcgaggc tgaagacaca ttgggtatgc
     3661 ccggacaagg tgttttcgag agaaaaatcc tcgtcaacac gttcccagat cttgacccag
     3721 tggtccttgc tccctgttgc cagatatcgc ccctgcgaat caaacacgag ggaaaaaacg
     3781 ccctcggcat agccacgcag ggtggttttc tcgcgccaga tcccgttgac ctcatgccag
     3841 atccggacac aactgtccga gccacccgaa gccaggatca tgccatccgg gg
//
LOCUS       DAOSHE010000172         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21427_length_3884_cov_3.288065, whole genome shotgun sequence.
ACCESSION   DAOSHE010000172 DAOSHE010000000
VERSION     DAOSHE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_3884_cov_3.288065"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLD82_05760"
     CDS             <1..422
                     /locus_tag="PLD82_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOG64926.1"
                     /translation="ADWRTLVFFTAMFIVMQSVWESGFFQDLMQRTGLDLGSVEGLMG
                     GGILISQLVSNVPFVALILPALGQAGAGTAQYLALAAGSTIAGNLLILGAASNIIVLQ
                     SAERHGAGFSFWEFARVGIPLTLLQAAVYWVWCSLVL"
     gene            577..846
                     /locus_tag="PLD82_05765"
                     /pseudo
     CDS             577..846
                     /locus_tag="PLD82_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            843..2951
                     /locus_tag="PLD82_05770"
     CDS             843..2951
                     /locus_tag="PLD82_05770"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG64927.1"
                     /translation="MSTITKARISGLSCAVCAAGIEKTLQAREGLAACRINFATGTIR
                     YPAEREAEVQDVICEIEPDVRIERDSGKHSGQPGPWHWRESWRLVLSGMLFVLVLFGE
                     QSWNEDGLQVVAWGLAFAGWLLAGWGVLRAAVRNLLHGRVFDENFLMTLATLGAFAIG
                     HLPEALGVMLFYHVGELAQDSAVQSSRSSIQALAGGIAEESRVVTDSGTHIRPAQEIP
                     PGVLLEVWPGERISHDGVLEGGPAWLDTSALTGESRPRMFDAGSDIQAGYLVVETRVR
                     LRVTRMLADSAVSRIMHLVEEATQRKAQTERFMTRFAAVYTPVVVLAAVLLATIPPLV
                     SAQAGFEDWFFRALVFLVISCPCALVVSIPLGYYAGIGAASRNGILVKGGIVLDALAR
                     VRSVVFDKTGTVTDGKYRVRRIIPGPGWTEDGVVQVAAAMEAHSNHPVARAVREAYSR
                     AGLPSLPEMAEIREVRGRGLTGRSRDGKMCVFGSAEFVCEACGCAAPQGQAGLYLAID
                     GVVVGAIEIDDGLRPSSRDAVTELRTLGVQHVALFSGDSSLRASQVGTLLGMDAAIGG
                     LLPEGKLKAMENLMQDRSMRTTVFVGDGINDAPVIKRADVGIAMGGLGSDAAIEAADA
                     VLVEDDPLLVSRAVRIARATRRVVWNNILFALSFKVVFLLLGGLGLASLWEAVIADVG
                     VALVALANSMRLVWGKRFSQ"
     gene            3159..>3884
                     /locus_tag="PLD82_05775"
     CDS             3159..>3884
                     /locus_tag="PLD82_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HOG64928.1"
                     /translation="MAFDTMRKDYEQRKAVAKPLTAGKFVKKEDAVRLLETVIRPGDR
                     VCLEGDNQKQADFLAKALVSVDPAKVNRLHMVQSSIVLPEHIQVFEKGIATDLDFAFS
                     GPQMKELYELVVSRKAKIGAIHTYLEIYGRYFLDLYPQVALIVAEEADEAGNLFTGFT
                     TEDTPIIAEATHFRSGIVIAQVKKKVASVPRVDIPGDWVDYVIETGEDFWMQALFTRD
                     PAKITDQQVLMAMMTIKGIYSEYN"
BASE COUNT          745 a         1003 c         1238 g          898 t
ORIGIN      
        1 atgcggactg gcggactctg gtgtttttta cggccatgtt tattgtcatg cagtccgtct
       61 gggaaagcgg attttttcag gacctgatgc agcggaccgg gctggatctc ggaagcgttg
      121 aaggcttgat gggcggcgga atcctgatca gccagcttgt atcgaacgtc ccgtttgtcg
      181 cgctcatcct tcctgcgttg ggacaggccg gcgccggtac tgcccaatat ctggcacttg
      241 ccgccgggag tacgattgcg gggaatctcc tgatcctcgg tgcagccagc aatatcatcg
      301 ttttgcaaag cgccgagcgg catggcgccg gcttcagttt ttgggaattt gcccgtgtcg
      361 ggattccctt gacactcctt caggcagcgg tctactgggt ctggtgttcg ctggtcctct
      421 gagtactttc ggggcctgag gatgttcatc aaaaagagcc ttgacaacaa ttaaaagatt
      481 gcttaattat atggcatgaa cagtcaagtg aacacacatg cttcctgtca gccggatgat
      541 cgtcgtctga cagaaaccga gtggcaggat tgcacgggga tggcagagct tttcaaggcc
      601 ctggcggacg agacacgaac caggattctg agcctcttgt tgaacaggga actgtgcgtt
      661 tgcgagctgg cagccagtct ggcgatgagt ctgccagctg tcagccatca cctgcgcttg
      721 ctcaagatgt tgcgactggt ggcaacacgt cgcgagggga agcaggtcta ctactcgctg
      781 ctggatcagc atgtggcgga tctgatcagg atcgcacgcg accacctgca cgagggggac
      841 cgatgagcac gatcacaaag gccaggatca gcgggttgag ctgcgcagtt tgtgccgccg
      901 gaattgaaaa gacgctccag gctcgtgagg gtctcgcagc gtgccgcatc aattttgcca
      961 ccgggacgat ccgctatccc gcagaacggg aggctgaggt tcaagacgtc atttgtgaga
     1021 ttgagccgga tgtccggatc gagcgggact ccgggaagca ctccggccag ccgggtccct
     1081 ggcattggag ggagagttgg cgtcttgtcc tgtcagggat gctctttgtg ctggtgcttt
     1141 ttggggaaca atcctggaac gaagacgggt tgcaggttgt agcctggggg ctggcttttg
     1201 cgggctggtt gctggccggc tggggggtgc ttcgggcggc cgtcaggaat ctcttgcatg
     1261 gtcgggtctt tgacgaaaac tttttgatga ctttggcgac gcttggggcc tttgccatcg
     1321 gacatctccc ggaagccctc ggtgtcatgc tcttttacca tgttggcgag ctggctcagg
     1381 attccgcggt ccagtcgagc aggagctcga tccaggctct ggccggaggg atcgccgagg
     1441 agagcagggt ggtgacggac tccgggacac acatccggcc cgcacaggaa attcctccgg
     1501 gagtactcct tgaagtgtgg cccggtgagc ggatctcaca cgatggtgtc ctcgagggcg
     1561 gaccggcctg gctggataca tcagccctga ccggcgaatc cagaccgcgc atgtttgacg
     1621 ccgggagtga catccaggcc gggtatcttg ttgtggagac gagagtccgg ctgcgcgtga
     1681 cacggatgct tgcggattct gccgtatcac ggatcatgca tcttgtggag gaagcaacac
     1741 agcgcaaggc ccaaacggaa cgtttcatga cgaggttcgc agcggtctac acacccgtcg
     1801 ttgtcctggc ggcagtcctg cttgcaacga tcccgcccct tgtgagcgca caggccggat
     1861 ttgaggactg gttctttcgc gcgcttgtct ttctcgtgat ttcctgtccc tgtgccctgg
     1921 ttgtctccat tcccctcggg tattacgcgg ggatcggggc ggcatcccgc aacgggatat
     1981 tggtcaaggg cgggatcgtc ctggatgcac tggcccgggt ccgttcggtc gtctttgaca
     2041 aaaccgggac ggttacagac gggaagtacc gggttcgaag gatcataccg ggtccggggt
     2101 ggactgagga tggtgttgtg caggtggccg ccgcgatgga agcacattcc aaccatcccg
     2161 tggcgcgggc tgtccgggag gcatactccc gggccggctt gccctccctg ccggagatgg
     2221 cagagattcg cgaggtgcgc gggcgtggtc tgacaggtcg atcccgggac gggaagatgt
     2281 gtgttttcgg atcagcagag tttgtctgcg aagcatgcgg atgtgctgct ccgcaaggac
     2341 aggccggatt gtatttggcc atcgatggag tcgttgtcgg agcaatcgag attgatgatg
     2401 gcctgcgtcc ttcatcccgg gatgctgtca ccgaactccg gacactcggt gtgcaacacg
     2461 tggcactctt cagtggcgat tcaagtttgc gagccagcca ggtcggaaca cttctcggca
     2521 tggatgcggc cattggcggg ttgttgccgg aaggcaagct caaggccatg gaaaatctca
     2581 tgcaggaccg gtccatgcga acgacggtct ttgtcgggga cggcatcaat gatgcacctg
     2641 tcatcaaacg ggcggatgtc ggaattgcca tgggcggttt ggggagtgat gccgccattg
     2701 aggctgcgga tgccgtcctc gtggaagacg atcccctcct ggtctcccgt gcggtgcgga
     2761 tcgcgagagc cacccgacgc gttgtctgga acaacattct gtttgccctc tcattcaagg
     2821 tggtgttctt gctgcttggt ggacttgggc ttgccagtct ttgggaggcc gtgattgcgg
     2881 atgtcggtgt ggctctggtt gcgttggcaa actcgatgcg gcttgtctgg ggaaaacgat
     2941 tttcacaatg aatcttttgt tcaactggta cagaacagac aggttggcat catctcagcc
     3001 aatctgtcac gacggccatc ttcggacaat gggctggaat ccccggatcc ggattgcaaa
     3061 tcgtcctctg atccagctca gtcttgcttt ttaccgggcg ttcctgctat tcttgaacgg
     3121 cagtctctga ttgtcattcc aaacctaagg cggattccat ggcatttgat acgatgcgga
     3181 aggattatga acagcggaag gcggtggcaa agccgttgac cgcagggaag tttgtcaaaa
     3241 aggaagatgc tgtccggctt cttgaaacgg tgatcaggcc gggtgaccgg gtctgtctcg
     3301 aaggggataa ccagaaacag gcggattttt tggccaaggc gctcgtgtcg gtcgatccgg
     3361 ccaaggtcaa cagattgcac atggtccagt cctccatagt cttgcccgag catatccagg
     3421 tttttgaaaa agggattgcc acggatctcg attttgcctt ttccggtccg cagatgaagg
     3481 aactctacga actggtggtt tcccgcaaag caaagatcgg ggccatccat acctatcttg
     3541 aaatctatgg acgctacttt ctcgacctct atccccaggt ggcactgatt gttgccgaag
     3601 aagcggatga ggccggcaat ctctttacgg gattcacgac cgaggatacc ccgatcattg
     3661 cagaggccac gcacttccgg tccgggatcg tgattgccca ggtcaaaaag aaggtggcga
     3721 gtgtgccgcg tgtcgatatc ccgggtgact gggtggatta cgtgatcgag acgggtgagg
     3781 atttctggat gcaggctctc ttcacccggg atccggccaa gatcaccgat cagcaggtcc
     3841 tgatggccat gatgaccatc aaggggatct actccgagta caat
//
LOCUS       DAOSHE010000173         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21443_length_3883_cov_3.194357, whole genome shotgun sequence.
ACCESSION   DAOSHE010000173 DAOSHE010000000
VERSION     DAOSHE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21443_length_3883_cov_3.194357"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PLD82_05780"
     CDS             complement(<1..157)
                     /locus_tag="PLD82_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64929.1"
                     /translation="MKLIVGLGNPGEAYRTTRHNLGFLALDRFAADAGISFDSKKKKA
                     LWGRGQYL"
     gene            complement(191..850)
                     /locus_tag="PLD82_05785"
     CDS             complement(191..850)
                     /locus_tag="PLD82_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG64930.1"
                     /translation="MEKIALAVEKRDQSGKCPNRRLRATGKIPAIMYGGKEQLMLAVD
                     RQEFERKFKRISENVLIDLAIKGGDSREVLIKDYQKDKVRGTLIHLDFYEVLKGHRVR
                     VKVPVKFEGTPVGVRVDKGMLETHLYQMEIDCEPKDIPESIVVDISGIAVNHSMHVRD
                     LQLPAGVVSHESPDTVVALVLSSNRVEGSGEADAAAADVSAVPAASAQKTEARAQDKK
                     K"
     gene            complement(958..1518)
                     /locus_tag="PLD82_05790"
     CDS             complement(958..1518)
                     /locus_tag="PLD82_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG64931.1"
                     /translation="MPGVAGHGHAPGDDTRGMLAVFSDSHGNLALLNKAVKSALGEGA
                     TVLVHLGDEYSDLAEVDTRSASVWRVPGLRCPEMRNPFIDNVLSFEFAGWRILAVHDC
                     SMAPGAEGARELVLYGHTHIPDCEKRPDGRWYCNPGHLKAVRDRGADAGYALVRALAD
                     GRLEVRFRTPEGKQAKSVDPQATLLD"
     gene            complement(1518..2396)
                     /gene="dapF"
                     /locus_tag="PLD82_05795"
     CDS             complement(1518..2396)
                     /gene="dapF"
                     /locus_tag="PLD82_05795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG64932.1"
                     /translation="MKFVKMQGAGNDYVYIDCFRNPVPEDPRSLAIKVSDRHFGIGSD
                     GLILILPSDKADARMRMFNSDGSESAMCGNGIRCVAKYVLDEGLAAGPGLDIETGRGV
                     LRLESEIGAAGLVERVCVDMGEAILDPDRIPASGFAGNPVVRETLRITDSGLDQAGLA
                     ELLEDGLACTLVSMGNPHCVIFVEDVATFPVGQVGPVIEHDAHFPERTNVEFVSIEGP
                     GEVRIRTWERGAGETLACGTGASAVTVALALTTGWRKPVRAHLLGGDLDLEWREDGHV
                     RMSGPAVLVFRGEMEL"
     gene            complement(2453..2737)
                     /gene="spoVG"
                     /locus_tag="PLD82_05800"
     CDS             complement(2453..2737)
                     /gene="spoVG"
                     /locus_tag="PLD82_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOG64933.1"
                     /translation="MKITDIRVKKVQGEGKLKAYVSVTFDECFVVHNMKVIDGKSGVF
                     VAMPSRKTNKGEFKDVAHPITVDFRTALQDSILEAYAVAPDSSPDDEDDE"
     gene            complement(2872..3540)
                     /locus_tag="PLD82_05805"
     CDS             complement(2872..3540)
                     /locus_tag="PLD82_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64934.1"
                     /translation="MGEWPVFKATAHFSYKDRDLDAGFFLVIRRDSTQNFTRVSIEGS
                     LDQNLWADLVAMGDSLSLYLPLDKRVLTGSRSRGSGFRMEGGVRFSLHELLLLAELRP
                     VLDTNQSARAESGPDGSSVLVLHAKDGNSATMVHFSPDGWITGIRSMQQGRERARIVY
                     EKRDVRTGIARKQTIISRDFGMEAVVWLSTWDPAWRYVPADGRLVLPEGVRRVSMENF
                     GRGR"
BASE COUNT          851 a         1253 c          980 g          799 t
ORIGIN      
        1 cgagatactg tccccgtccc cagagcgcct tctttttttt ggaatcaaaa gaaatccccg
       61 cgtcggccgc aaaacggtcg agcgcgagga atccgagatt atgacgtgtc gtgcgatagg
      121 cttcgccggg attcccgaga ccaacaatca atttcatgta ttgatctccg cccggccggt
      181 cacgccgtca ttatttcttt ttgtcctgtg cacgggcttc ggtcttctgt gcggaggcag
      241 cgggaacagc ggatacatcc gctgctgcag catcggcctc gccggaaccc tcgacacggt
      301 ttgacgaaag aaccagggcc acaacggtgt ccggggactc gtgcgaaaca actcctgccg
      361 gaagctggag atcgcgtacg tgcatcgaat ggttgacagc aatcccggag atgtcgacaa
      421 caatgctctc cggaatatcc ttgggctcgc agtcgatctc catctgatac agatgtgtct
      481 ccagcatacc cttgtccaca cgaacaccaa cgggtgtccc ctcgaacttg accggaacct
      541 tgacgcgcac cctgtgtccc ttgaggactt cgtagaaatc gagatggatc agtgtcccgc
      601 gaaccttgtc cttctgataa tccttgatca gaacctcacg ggaatcccca cccttgatgg
      661 caaggtcgat caaaacgttt tcggagatgc gtttgaactt gcgttcgaac tcctggcggt
      721 caacagccag catgagctgt tccttgcctc cgtacatgat tgcaggaatc ttccctgtgg
      781 cgcgcagcct gcgattggga cattttccgc tctggtctct tttctcgact gcgagagcaa
      841 tcttttccat atcgtccatt ctcctcaaac tgatacagac cgccgcgcgc acatccaggc
      901 cacatgcagg gtcatcgttt catagattgt atggaaatct atccatcgga ctggtcatca
      961 gtcaagcaga gttgcttgcg gatcgacgct ttttgcctgt tttccctcag gtgtgcgaaa
     1021 ccggacctcg agtcgtccgt ccgccaaagc gcgtaccagg gcatacccgg catcagcccc
     1081 cctgtcgcgg accgctttga gatgcccggg attgcagtac caacgcccgt ccggacgttt
     1141 ttcgcagtcc gggatatggg tatgcccata gagcacaagc tcacgcgccc cctcggcccc
     1201 gggtgccata ctgcagtcat gcacagccag aatccgccac cccgcgaatt cgaaggaaag
     1261 cacgttgtcg ataaaaggat tgcgcatctc gggacagcgc agacccggca cacgccagac
     1321 cgatgccgag cgggtatcaa cctccgccag atccgaatac tcgtctccaa gatgcacgag
     1381 gacggtggca ccctccccca acgcgctctt gacagccttg ttcagaaggg ccaggtttcc
     1441 gtgggaatcc gagaaaacgg ccagcatgcc ccgggtatcg tctcccggtg catggccatg
     1501 tcccgcgaca cccggcatca gagctccatc tccccacgaa agacgagaac ggccggaccg
     1561 ctcatgcgta catgcccatc ctcccgccat tcaagatcga ggtccccgcc caaaagatgc
     1621 gcacgaaccg gtttgcgcca accggtcgtc agggccaggg ccaccgtaac cgccgaagct
     1681 ccggtcccgc aggccagtgt ctcgcctgct ccccgctccc aggtgcggat acgcacctcg
     1741 cctggacctt cgatggacac aaattcgaca tttgtccgtt ccgggaaatg ggcgtcatgt
     1801 tcgatcaccg gcccgacctg cccgacagga aatgtcgcga catcttcgac aaagattaca
     1861 caatggggat ttcccatcga gaccagggta caggccaagc cgtcttcaag aagttcggcc
     1921 agccccgctt gatcaagacc ggaatccgtg attctgagcg tctcgcgcac caccgggttc
     1981 ccggcaaagc cactggcagg aatccggtcc gggtcgagga tggcctcacc catatcgaca
     2041 cagacccgct ccaccagacc ggccgcaccg atctcgctct ccagccggag aacgccccgg
     2101 cccgtttcga tatccagtcc gggtccggcg gcaagtccct catccagaac gtatttggcg
     2161 acacaacgga ttccgtttcc gcacatcgcg gactcgctcc cgtcgctgtt gaacatccgc
     2221 atgcgtgcat cggccttgtc cgatggaaga atcaggatca ggccgtcgga tccgatcccg
     2281 aaatgccggt cactcacctt gatggccaac gaccggggat cctcgggcac cggattgcgg
     2341 aagcagtcga tatagacgta atcgtttccg gcaccctgca ttttgacaaa cttcatacat
     2401 caccccgtac ggaaagactc aacccccgtc acggggattc ccggaaaata tatcattcat
     2461 cgtcttcgtc gtccggagac gaatcgggcg caacggcgta ggcttcgaga atcgaatcct
     2521 ggagtgcggt ccggaaatcc acagtgatcg ggtgcgctac atccttgaac tcgcccttat
     2581 tggtcttgcg gctgggcatc gcaacgaaaa ctccgctctt cccgtcaatc accttcatgt
     2641 tgtgaacgac aaagcactca tcaaatgtta ccgaaacata ggccttgagc ttgccctctc
     2701 cctgcacctt cttgacgcgg atatccgtaa ttttcattgc tgtaacccat cctcttatcc
     2761 gtttgcacgc ctcgtattga tcttctgtgt accaacagaa gccctcgcac gtatgatatc
     2821 gcaggaccga agtttctgca ataccgttct tccattattt ccggttcggc cctatctccc
     2881 tcgcccgaaa ttctccatgc taaccctgcg gacaccctcc ggaagcacca gtctcccgtc
     2941 tgcagggaca tagcgccagg ccggatccca ggtcgagagc cagacaacgg cctccatccc
     3001 gaaatcccgg gaaatgatcg tctgcttgcg ggcaattccc gtccgcacat cccgtttttc
     3061 gtagacaatc cgggcccgct cccgcccctg ctgcatggag cgaataccgg tgatccatcc
     3121 atccggagaa aaatgcacca tggtggcgga attcccgtcc ttggcatgca aaaccagcac
     3181 ggatgagcca tccggaccgg attcggcacg ggcggactgg tttgtgtcaa gtacgggacg
     3241 caattcggcc agcagaagca gttcatgcag actgaagcgc acgccaccct ccatgcgaaa
     3301 cccgcttccc cgggaacggc ttccggtcaa cacccgttta tccagcggga gatagaggga
     3361 gagactatcc cccatggcca cgagatcggc ccagagattc tggtccagcg atccctcgat
     3421 cgagaccctg gtgaaatttt gtgtgctgtc acgccggatc acgagaaaaa agccggcatc
     3481 gagatcacga tccttgtatg aaaaatgcgc tgtcgccttg aagacaggcc attcacccat
     3541 cctgcgggac tcagcatcca ccaaacgcag gagatcagcc acagattttt tttgaggaac
     3601 agaagacttc tccggtacgc ctttacagcc tgcacataaa atcgtaataa gtgcaagaac
     3661 tacactcatt actgaaatca gtcgggatac tttcagacag cagatcaccg ccatcggccg
     3721 cttccccgat cgacagcttc atgctccttg agcatccgca gactgcgctg ctctccgggc
     3781 acaagaacct gcacccgtct ccaggcagca agcgcgctcc tcttcctgcc ggtagcgaga
     3841 aaggcctgcc cttgacagag gagcagttcg acctgccgcc ccg
//
LOCUS       DAOSHE010000174         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21449_length_3882_cov_4.846616, whole genome shotgun sequence.
ACCESSION   DAOSHE010000174 DAOSHE010000000
VERSION     DAOSHE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21449_length_3882_cov_4.846616"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLD82_05810"
     CDS             complement(<1..268)
                     /locus_tag="PLD82_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019961811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOG64935.1"
                     /translation="MIAKILSKNAPPVEYGRRFAFFQIVDHPEKGSTPTIPDETFLIS
                     RRESDPWTAVIRWARHYGVRLACAESCTGGLVSAGLTAIAGASRV"
     gene            complement(286..636)
                     /locus_tag="PLD82_05815"
     CDS             complement(286..636)
                     /locus_tag="PLD82_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64936.1"
                     /translation="MTVTEICLIIIASSLVLGLGILLVAVLAVSRAYKKKVLPLIQKT
                     NGVLDNLSAVTLAIRDQVDELRTVMDDITYRTRVMAINVQEKIIPTVSDVVSSLSGVF
                     RFFSGIFGRNQNSR"
     gene            complement(663..878)
                     /locus_tag="PLD82_05820"
     CDS             complement(663..878)
                     /locus_tag="PLD82_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOG64937.1"
                     /translation="MNENGKTAVAFLLGVIAGGVLGIMFAPKSGKETRESLHKYIKSA
                     EEKLARLRHRRDQMDDDEQANITDEQA"
     gene            complement(965..2377)
                     /locus_tag="PLD82_05825"
     CDS             complement(965..2377)
                     /locus_tag="PLD82_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG64938.1"
                     /translation="MGKRKLLAGTVGMILLAVCLGFFVNGQVIGRDSVYENFELFSKA
                     FQNIRNYYYDTDKIKSKDLIYGGIRGMLEALEDEHTSFLPPAELKSLSEETSGEFGGL
                     GIMIGIREKVLTVISPIYGTPAWEKHIQPGDMIMSIDGVTTKDIPLVDAVRKLRGPVG
                     STVTITILRPNQDESFDVVIKRALIRIETVKTAYLEREGLGYIKITQFSENTEELLRK
                     AWAEFSQKQIKGVILDLRNNPGGLLSSATEVANLFISEGLLVYTKGREAEPVRRFEAS
                     RARAIFDTRIPLIVMVNKGSASASEIVAGALQDTGRAILLGENTFGKGSVQTIRPMMD
                     GSAIKLTVALYYTPAGRQIHKKGLVPDIVVPVPEYSKAEQKAVLRLMNEDHLARFLKE
                     HPEYTAKHLADFVEALRKKDIVLPEEEVERWIYWEKNKVGEPELVNLRFDIQLRKAVE
                     LFGNATIKAPGKIRVFTEVK"
     gene            complement(2493..3641)
                     /locus_tag="PLD82_05830"
     CDS             complement(2493..3641)
                     /locus_tag="PLD82_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG64939.1"
                     /translation="MSVFEFNIPARVIVGLNVVNRLPEIIQDYGERVMIVTSDTMVQQ
                     GILRRVQSLAESKSYGVIVFDKCSADAGSDVVDEGVLLARESRTNVVIGLGGANTLNV
                     AKAIACIAGNDGYIADYFAGKTAKRKKIAYIEIPSTHGICHGLLDSFFIRDVDDNIRK
                     EYRSRFNYAEVVLVDPKLTASLAPKFVVAIGIEVMAHACEAYISKAASIMTESYIGNA
                     IELVYANLKQAVIEPENMVARQALSTAGVFASIALNSSRPGTTWALALALSTRLGLYR
                     GIGVSIILPHVMEFNLTASPNKYVRIARSFGEDVNDISVVEAAIKAIEAVRKLLFDLN
                     VPQRLKNFEIEPDGFDEVVRDARTYDFLSVLPRSVTYDDLLNILNAAY"
BASE COUNT          912 a         1124 c          923 g          923 t
ORIGIN      
        1 caacccgcga agccccggcg atggccgtca aaccggcgga gacaagtccc ccggtacagg
       61 actcggcaca ggccagcctg actccgtaat gacgggccca acggatgacc gctgtccaag
      121 gatcactctc ccgacgggag atcaagaaag tctcatccgg aatcgtgggt gttgatccct
      181 tctccggatg atcgacgatc tgaaaaaagg caaaccgcct gccgtactcg acaggcggag
      241 cgtttttgga caagattttg gcaatcatcg tgaagaagtt ttcatctagc gcgagttctg
      301 gttgcgcccg aaaattccgc tgaaaaacct gaacacgccc gaaagggacg aaacaacatc
      361 actcacggtc gggatgatct tttcctgtac gttgattgcc atcaccctgg tccgataggt
      421 gatgtcatcc atcacagtcc ggagttcatc gacctggtca cgaatggcaa gggtcaccgc
      481 tgacagatta tccagcacac cgtttgtctt ttgaataagc ggaagaacct ttttcttgta
      541 ggccctggat accgccagca ccgcaaccag aagaataccc agccccagta caagggaaga
      601 ggcaatgatg atgaggcaaa tctcagtcac tgtcatggtg tttcctgtct cttgccaggc
      661 tctcaggcct gctcgtctgt tatattcgcc tgctcgtcat cgtccatctg atcacgcctg
      721 tgcctcaatc gggccaactt ttcctcggca gacttgatgt acttgtgcag actctcacgg
      781 gtttctttgc cggactttgg agcaaacatg attcccaaca cccctccggc aataactccg
      841 agaagaaagg ctactgctgt ttttccgttt tcattcattc cggcctcctc gcctgatcac
      901 aggcgtatac tcacatcctg caccctgatc aggcccttgt caaggccggc attccgggca
      961 tgtatcattt tacttcggta aaaacccgaa tctttccggg agccttgatc gtcgcgtttc
     1021 caaagagttc gaccgctttc cgaagctgga tatcaaaccg gagattgacc agttccggct
     1081 caccaacctt gttcttctcc cagtagatcc agcgctcaac ctcttcctcg ggcaatacaa
     1141 tgtctttttt ccgcaacgct tcgacaaaat ctgccaagtg tttcgccgta tactccggat
     1201 gctctttcaa aaaacgggca aggtgatcct cattcatcag acgaagcaca gccttttgct
     1261 ctgctttgga atactccggc acgggaacca caatatccgg gacaagtcct tttttatgga
     1321 tctgccggcc agccggtgtg taatacaggg ccaccgtcag ttttatcgcg gacccgtcca
     1381 tcatgggacg gattgtctgg actgaaccct ttccgaaggt attctcaccc agcaatatgg
     1441 cacgtcccgt atcctggagg gctccagcaa cgatctcgga tgctgaagcc gaccctttat
     1501 tgaccatcac aatcagggga atcctggtat caaagatggc ccgggcacgg gaagcttcga
     1561 acctccggac aggttctgct tcgcgtccct tcgtgtaaac aagcagacct tcggagatga
     1621 acagattggc gacctcggtt gcggaactga gcaacccgcc gggattgttc cgcaagtcaa
     1681 gaatcacacc cttgatctgt ttttgcgaga actctgccca cgccttgcgc aacagctctt
     1741 cagtgttttc ggaaaattgg gtgatcttga tatagccgag accttcccgc tcgagatacg
     1801 cggttttgac agtctcgatc ctgatcaatg cccttttgat gacaacatca aaagactcat
     1861 cctggttggg ccggagtatg gtgatggtca cagtgcttcc gacaggtccc ctgagtttcc
     1921 ggacagcatc cacaagcggg atgtccttgg tcgtgacccc gtcgatcgac atgatcatgt
     1981 cgccgggctg aatatgcttt tcccaggcgg gtgtcccgta gatcgggctg atgaccgtca
     2041 ggactttttc ccggattccg atcatgattc ccagcccacc aaactcgccg cttgtctcct
     2101 cactgagact cttcagttcg gcaggtggca gaaacgaggt atgctcgtct tcaagggcct
     2161 cgagcatgcc acggatcccg ccataaatca ggtcctttga cttgatttta tcggtatcat
     2221 aatagtagtt gcggatattc tggaaggcct tgctgaagag ctcgaaattt tcatacaccg
     2281 aatcgcggcc gatcacctgt ccgttcacaa agaaaccgag acagacagcc agcaatatca
     2341 tgcccacagt cccggcgagc aattttctct ttcccatcct gtcctccaca acgccatgcc
     2401 cccgacacaa accatacggg acaatctcag tataaggtag tctgccggaa gttttttttc
     2461 cagaagaaag catgcatcag agccatacgg aatcaatacg ctgcgttcag gatgttcaac
     2521 agatcgtcat aggtcacaga ccgcggaaga acacttaaaa aatcatatgt tctggcatca
     2581 cggacaacct catcaaatcc atccggttcg atttcaaagt ttttgagccg ttgcgggaca
     2641 ttcaggtcaa aaagcagctt gcggacagcc tcgatcgcct tgatggccgc ttccacaacg
     2701 ctgatatcgt tcacgtcttc cccgaatgag cgtgcaattc ttacatattt gttgggggat
     2761 gccgtcaggt tgaattccat cacgtgcgga aggatgatgg agacgccgat tccccgatac
     2821 agccccaacc gtgtcgacaa agccagggcc agggcccacg tcgttcctgg ccggctggaa
     2881 ttgagcgcaa tggacgcaaa gactccggcc gtggagaggg cctgccgggc caccatgttt
     2941 tcaggttcga tcacggcctg tttgagattg gcgtacacca gctcgatggc gttcccgatg
     3001 taggattcgg tcatgattga agcggctttt gaaatatagg cctcacaggc atgagccatg
     3061 acttcgatac cgatcgcaac gacaaatttg ggagcaagac tggctgtgag tttcggatcc
     3121 accaggacaa cctcggcgta attgaagcgg ctccggtact ccttcctgat attgtcatcc
     3181 acatctctta taaaaaagga atccagaagg ccatggcaaa ttccatgcgt cgagggaatc
     3241 tcgatatagg ctattttctt tcgttttgcc gttttcccgg caaaataatc cgcaatatat
     3301 ccatcgttcc cggcaataca ggcaatcgct ttggcgacat tcagggtatt ggcgccacca
     3361 agcccgataa ccacattggt ccgactctcc ctcgccaaga gaacgccctc atccaccaca
     3421 tcgcttccgg cgtcagcact gcacttgtcg aagacaatca ctccgtacga cttgctttcg
     3481 gcaagactct ggacccgacg caggataccc tgctgcacca tggtatcact ggtgacgatc
     3541 atcacccgtt cgccataatc ctgaatgatt tcgggcagcc tgttgaccac gttgaggccg
     3601 acaatcacac gtgccgggat attgaactcg aaaacactca tgatcgcccg ccctgtcagt
     3661 gcattctaca cctgttgttc ttcatcgcag caaacaaaga aaaggagcac gcatgaccgt
     3721 agctcctttg tctccgcttt ttgtcctcat ggcatcgcca caaagatttc cggatatcag
     3781 ccgacaccga catcacgcca gccggcacct gtcagttctg accgccgaga ctctccgcaa
     3841 tccgtgatgc caactcacgc agaacactct cgttgtacag gg
//
LOCUS       DAOSHE010000175         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21510_length_3874_cov_3.292747, whole genome shotgun sequence.
ACCESSION   DAOSHE010000175 DAOSHE010000000
VERSION     DAOSHE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21510_length_3874_cov_3.292747"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="PLD82_05835"
     CDS             complement(<1..1532)
                     /locus_tag="PLD82_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOG64940.1"
                     /translation="MHLLPLNRLKKRVLRNNTTAWLLSSVLGYLIINAILRLWILGGF
                     LSYLPDRILNPVYAFGFHVIPGAFGEILSLSLLLLVLAIPGVLGAKKISSLLMVSSVI
                     LTIAGLHYFMYAVDWFTPQDLRLTGGVSFESSLDLISGSFSIPIIAIQLFSGATGFLL
                     LQSLFFRRLTNVPHSLRTRNNSFRNVTYLLATLLTLSVLYLILPVSWYHKCAINPVIH
                     FAERIIPDNTPPLNIAEVQFAAQEAGFPDCLSSSDQYSWTRSNDPLLRKPNMRPHAQS
                     HEYRHPNIFIFVLESLAFQILEHKSLTRTSFQKFRSESVEFSYFFANGFPTADGHEAL
                     LYSVFPPLIDGYNFRQHQMISLAHILKKNGFATQAYLCSQHESAKQYRFFEKAGFGTI
                     YDTHFFNIPEKGDTAAETILLSAMVSNASAWPNSRPVMMFFSSAIGHSPFKYYPKNSS
                     VEKVIKNATTLEERYLNQINFILESVATTILSIRRTEAGANAIFIITGDHAPHAKLAY
                     EK"
     gene            complement(1617..2495)
                     /gene="rfbD"
                     /locus_tag="PLD82_05840"
     CDS             complement(1617..2495)
                     /gene="rfbD"
                     /locus_tag="PLD82_05840"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG64941.1"
                     /translation="MIWIIGSNGMLGSRLCGMLSATGMAFTGTDVETSILDPQVLSRF
                     AHEHDSIRCMINCSAYTNVDKAESDADTAYAINERGVRNLAEIAARLDIPFIHISTDY
                     VFDGKGTLPYREDDPTCPISVYGASKLAGERAAIAACSKTVVIRTSWLYDSHGKNFFL
                     TMLRLFREKGAVNVVSDQQGNPTWAADLARAILSVAHILQAAPLENMYGVFHFSNEGI
                     TTWYDFAREIASQACASHILATNPTVIPVTSDAWPTPVKRPAWSALDKTKIKNTFGIT
                     VPRWELSLASCLILDQ"
     gene            complement(2492..3043)
                     /gene="rfbC"
                     /locus_tag="PLD82_05845"
     CDS             complement(2492..3043)
                     /gene="rfbC"
                     /locus_tag="PLD82_05845"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG64942.1"
                     /translation="MTLEQTPLSGLIIIHPKVFGDERGFFMETWSQPVFSSLGIDTVF
                     LQDNHSRSCRGTVRGLHFQSTPGQAKLVRCVKGAVWDVAVDLRTDSPTFGRWHAVRLD
                     EDNRAMLYIPIGFAHGFCVLSESADFVYKVSSIYNPETECGIAWNDPDIGIQWPTETP
                     LLSDRDKHNCGLRDWLASKDQMV"
     gene            complement(3040..>3874)
                     /gene="rfbA"
                     /locus_tag="PLD82_05850"
     CDS             complement(3040..>3874)
                     /gene="rfbA"
                     /locus_tag="PLD82_05850"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOG64943.1"
                     /translation="QILPVYNKPMIYYPLSTLMQASIRDILIISTPEHLGFYQSLFGD
                     GSRLGLSISYREQPEANGLAQAFVIGEDFIGADPVALVLGDNIFYGTDFYGFLDKFDG
                     TPDGGLIFGYRVADPERYGVVEFDQNDNVISIEEKPTHPKSHYVVPGLYFYDNSVVAI
                     SKELKPSPRGEYEITDVNNEYLRRGKLKVALINRGNAWLDTGTHRSLLEASSFVQTIE
                     DRKGQMISCLEEIALQKGFIDSDKFKSAMSFYPSGNEYHDYLKIVLVEYLEKTPKRAH
                     P"
BASE COUNT          971 a          865 c         1077 g          961 t
ORIGIN      
        1 attttttcat atgcgagttt agcatgtggt gcatgatctc cggtgatgat aaatatggcg
       61 ttggcacctg cctcggttcg acgaatgctg agtatcgttg tcgccactga ttcaagaatg
      121 aaatttatct ggtttaggta tcgttcttcc agtgtggtgg cgtttttgat cactttttcc
      181 actgagctgt ttttcggata gtatttgaac ggagagtggc cgatggcgct ggagaaaaac
      241 atcatgacgg gccttgaatt tggccaggcc gaagcattgg agaccatggc ggacagaaga
      301 atcgtctctg cagccgtgtc gcccttttcc gggatattaa aaaagtgtgt gtcatagatt
      361 gtgccaaaac cggccttctc aaaaaatctg tattgcttgg cagattcgtg ctggctgcac
      421 aggtatgcct gggttgcgaa tccgtttttt ttgaggatat gggccaagga tatcatctga
      481 tgctggcgaa aattgtaccc atcgataagc ggaggaaaaa ccgagtagag caaggcttca
      541 tggccatccg ctgtcggaaa tccgttcgca aaaaaatagc tgaactcgac cgactcacta
      601 cgaaatttct ggaagcttgt gcgggtcagg gatttgtgct caaggatttg aaaggccagc
      661 gactcgagaa cgaatataaa aatatttggg tgcctgtatt catgactctg tgcatgagga
      721 cgcatattcg gcttgcgcaa gagaggatca ttgctgcggg tccatgaata ctggtcagaa
      781 gaagacaggc agtccggaaa tcctgcttcc tgcgctgcaa attgtacttc agcaatattc
      841 aggggaggtg tgttgtccgg gatgattctt tctgcaaagt gtattaccgg gttgatggcg
      901 catttgtgat accaggatac cggcagaatc agatacaaga cagacagagt cagcaatgtg
      961 gccagaagat atgtgacgtt acgaaaagag ttgtttcggg tccgaagtga atgcgggaca
     1021 ttggttagcc ggcggaaaaa cagcgattgt agcaggagga agcctgtggc ccctgagaag
     1081 agctgtatag cgataatcgg gatggaaaag ctgccactga tcagatcaag gctgctttca
     1141 aaacttaccc ccccggtcag gcggaggtct tgcggggtaa accagtcaac ggcatacatg
     1201 aaatagtgca ggccagcaat agtcaaaatg accgatgaaa ccataagtag cgaggatatt
     1261 tttttggccc ccaatactcc tggtattgcc aggacaagga ggagcaagct tagggacagg
     1321 atttcgccaa aggcaccggg gatgacatga aacccgaacg cataaacggg gttgagaatc
     1381 cggtcgggaa gataggataa aaatccaccc aaaatccaga ggcggagtat ggcattaata
     1441 attaggtatc caagtaccga cgagagcagc caagctgtcg tgttgtttcg caataccctt
     1501 tttttcagtc tgtttaatgg gagcaggtgc atacatactc ctccgaatga ttgtatggcc
     1561 tgcatgcggc ttgtggacaa tttaaccacc ttgtccccca aaaagggcca gttttgttac
     1621 tgatctaaga tcaggcagga tgcaagggat agttcccagc gtgggacggt tataccaaat
     1681 gtgtttttga tttttgtctt gtccagggca gaccaggcag gtcgtttgac cggagttggc
     1741 catgcgtcag aagtgaccgg aatgaccgtc gggtttgtgg caaggatgtg agatgcacat
     1801 gcctgggaag caatttcccg ggcaaaatcg taccaggtcg taatgccttc gttgctgaag
     1861 tgaaacactc cgtacatgtt ttcgagcggg gctgcttgca ggatgtgtgc gacagacaat
     1921 atagcgcggg ccagatctgc tgcccatgtc gggttgcctt gctgatccga cacgacgttg
     1981 actgcaccct tttcgcgaaa gagacggagc atggtcagga aaaagttttt tccgtgagag
     2041 tcatagagcc atgacgtccg aatgacgacg gtttttgaac atgctgcaat agcggcacgc
     2101 tcgccagcca gttttgaagc accatacacc gagatagggc aggttggatc gtcttcgcga
     2161 tacgggagcg ttccttttcc gtcgaaaaca tagtctgtgg aaatatgaat gaaggggata
     2221 tcaagccgtg ccgcgatttc ggcaaggttg cgcacccctc tctcgttaat ggcgtaagcg
     2281 gtatcagcat cgctctcggc tttgtctacg ttggtgtacg cactgcagtt aatcatgcag
     2341 cgaatggaat catgctcatg tgcaaaacgg gacagaactt gcgggtcgag gatgctggtc
     2401 tcgacatccg ttccggtaaa tgccattcct gttgctgaaa gcatgccaca gagtctggat
     2461 ccaagcattc cgtttgagcc gataatccat atcataccat ttggtccttt gaggcaagcc
     2521 aatcacggag tccgcagttg tgcttgtccc gatcggaaag aagtggtgtc tcggttggcc
     2581 attgaatacc gatatcagga tcattccagg caatcccgca ttctgtttca ggattataga
     2641 tgctcgagac tttatatacg aaatcggcgc tttcggagag gacacaaaat ccgtgggcaa
     2701 acccgatcgg gatataaagc atggccctgt tatcctcgtc aagtcggaca gcatgccatc
     2761 tgccaaaggt cggggaatcg gtgcgaagat caacagcaac gtcccagact gcgcctttga
     2821 cgcagcggac gagctttgcc tgccccggtg ttgactggaa atgaagccca cgcactgtcc
     2881 ctctgcacga acgggagtga ttgtcctgaa gaaacacagt gtcaatccca aggctgctga
     2941 aaaccggttg actccaggtt tccataaaaa agcccctctc gtcaccgaac actttggggt
     3001 gaataatgat caggccggac aggggcgttt gttcaagggt catggatggg cccttttagg
     3061 tgttttttca agatactcga ccaggacaat cttgagatag tcatggtatt cgttccccga
     3121 aggataaaag gacatggcgc ttttgaattt atcgctgtca atgaatcctt tttgcagtgc
     3181 gatttcttca aggcaggaga tcatctggcc ctttcggtct tcgatggtct ggacaaagct
     3241 tgacgcttcg agcagactgc ggtgagtgcc ggtgtcaagc caggcgttac cgcggttgat
     3301 caaggccacc ttgagttttc cccggcgaag gtattcattg ttgacatccg tgatctcgta
     3361 ttcgccgcgt ggcgacggct tgagttcctt cgaaattgca actacggaat tatcataaaa
     3421 gtacagaccc gggacgacat agtgggattt tgggtgggtc ggtttttctt caatggatat
     3481 gacgttgtcg ttttgatcaa actcgacaac gccatagcgt tcggggtcgg ctacccggta
     3541 tccgaagatg agcccgccat caggggttcc gtcaaatttg tcgaggaagc cgtaaaaatc
     3601 agtcccatag aagatgttgt ctccgaggac aagtgcaacc gggtcagcgc cgataaaatc
     3661 ctcacctatg acgaatgctt gagccaggcc gttggcctct ggctgttcgc gatacgagat
     3721 tgacaggccc agccgggagc cgtcaccgaa gagtgactgg taaaagccaa gatgttccgg
     3781 tgtggaaatg atcaggatat cccggattga tgcctgcatc agggtcgaga gcgggtaata
     3841 gatcatgggt ttgttataga ccggaagaat ctgt
//
LOCUS       DAOSHE010000176         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21519_length_3873_cov_4.593504, whole genome shotgun sequence.
ACCESSION   DAOSHE010000176 DAOSHE010000000
VERSION     DAOSHE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21519_length_3873_cov_4.593504"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLD82_05855"
     CDS             complement(<1..289)
                     /locus_tag="PLD82_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOG64944.1"
                     /translation="MSVFDILKVRVAEGYQAIRDPRKQPLDPKFRGIPLLDGAACGKC
                     DTPGECAAVCPASAITFEPLSIDLGRCVLCGDCAAACSHGGIRFSSEHRMST"
     gene            complement(308..1873)
                     /locus_tag="PLD82_05860"
     CDS             complement(308..1873)
                     /locus_tag="PLD82_05860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG64945.1"
                     /translation="MNSGWSRTRNLRPVSIHDIPRFPLSRFREDIISLCSVSDRDRAR
                     PVTLFGVRRWEGIELVCVLARDSRAELLVASCMVQPGSEYPTMVREVPALHIFERELY
                     EEFGIVPRHHPWLKPVRARGGGWFVDGQGITRKAGDPQAGACSPKRRSAVERYPFFPV
                     QGDSVHQVGVGPVHAGIIEPGHFRFHCRGEEILHLEIALGYQHRGVEDLLVRKADQAV
                     RVSESIAGDSVIAHATATANAMEVLGNCVPSLRALTIRSIALEMERAAIHIGDLGALA
                     GDVAFLPGMSFYGATRTLVINGLLSFSGSRFGRGLIRPGGVAHDINDEQRSRLDVALS
                     RTCKDTERLSEAMFASPSVLSRFERTGIVSLEEAQSMGFSGPTARASGVPCDVRFDHP
                     FGLYRLMPVHPVVLDGGDVFSRAWIRYAEAITSLEYVRERLAALPEGSLVRRLAPAAP
                     SRLVVSMVEGWRGEIVHAVVTDEDGRVCRHRVQDPSLANWYALALAVRGNGISDFPIC
                     NKSFNLSYCGNDL"
     gene            complement(1870..3300)
                     /locus_tag="PLD82_05865"
     CDS             complement(1870..3300)
                     /locus_tag="PLD82_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG64946.1"
                     /translation="MVIPVLFFLPLTLAVLSFLMRRPALDRYLVLLHAMGHLAGVAWM
                     ALEPCSWAGYFETDPPAVLVLALLAIVYLAVAVKRFLDERGMHHDGHEAWHSLQTTAL
                     MIFIGAMTAAALTNHAAMLWVFVEGTTLASAPLIYSSRTKTALEATWKYIFICSIGIA
                     LAFIGIILLMMGMEPGASLHFRDLEANAGKIDPFWLKFAFPFILVGFGTKAGFAPVHA
                     WLPDAHSEAPSGISALLSATLLNTALLAVLRFGNILDAAGYGVFVRDALVVTGFLSLF
                     VAAVYMLRVENYKRMLAYSSIENMGIMAIGFGLGGAGAVAAVLHMVAHSLVKGAFFLT
                     AGDILHAWKRRDIAGAGGVLATWPRTGWVWLTAFVAISGLPPFAAFFSEIMLMSALLE
                     AGYPVLLALFLLLLTVIIWGMGRGVLAVAMGPARPDLAGHRSSAIWPQVLLLGLAIAI
                     VVAMPQPVETFFRDVAAILGVNGGQR"
     gene            complement(3303..>3873)
                     /locus_tag="PLD82_05870"
     CDS             complement(3303..>3873)
                     /locus_tag="PLD82_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64947.1"
                     /translation="HAVIRALSIQGLLLFVLVLDDAHAFDTINLVFLIIETLVVKAVV
                     IPLFLQRIIVRNHIYREIEPYIPNFYSLLVASIILGGGFLLEHLSRGVTSGTKPLALG
                     VSLAVIVTALFLIISRKKLITHVLGFLAMENGVFMLTMAVAREHTFAVNVGLLLDVFL
                     AVFLLGLFMNRIASSYEEMSVDELSDLKD"
BASE COUNT          919 a         1200 c         1134 g          620 t
ORIGIN      
        1 acgtcgacat ccggtgctca ctggaaaacc ggatcccgcc atgggagcag gcagccgcgc
       61 aatccccgca caacacacat cgacccagat cgatcgaaag gggctcaaac gtgatggcgg
      121 atgccgggca gaccgcggca cattccccgg gagtatcgca cttcccgcag gccgcgccat
      181 ccagcagggg aatcccgcgg aacttgggat ccagcggctg tttgcgcgga tccctgatgg
      241 cctggtaccc ttcggccacc ctgaccttga gtatgtcaaa cacgctcatg acacaagcct
      301 ccgcacatta caagtcgttt ccgcaataac tcaggttgaa gctcttgttg caaatcggaa
      361 aatccgaaat cccattgccg cgaacggcca gcgccaaggc ataccagttg gccagtgacg
      421 ggtcctggac cctgtgccgg cagacccgcc catcctcgtc cgtcaccacg gcgtgcacga
      481 tttccccgcg ccagccttcg accatcgaaa ccaccagccg ggatggtgcc gccggagcaa
      541 gccgccggac cagactcccc tccggcaaag cagccagccg ttccctgacg tactccaaag
      601 atgtgatcgc ctcggcatag cggatccagg cccgggagaa gacatcaccc ccgtccagca
      661 ccacgggatg gaccggcatc aatcgataaa gaccgaaggg atggtcgaag cggacatcgc
      721 agggcacacc gctcgcccgg gctgtcggtc cgctgaatcc catgctttgc gcctcctcga
      781 gggagacaat tcccgtccgt tcaaagcgcg agagtaccga gggagaggca aacatcgctt
      841 cggagagacg ctcggtgtcc ttgcaggtcc gggagagcgc cacatccagc ctggagcgtt
      901 gctcgtcatt gatgtcgtgg gccaccccac cgggccggat caggccgcgg ccaaacctgc
      961 tccccgaaaa cgagaggagc ccgttgatca ccagggtcct cgttgcaccg tagaacgaca
     1021 tccccggaag gaaggcaaca tccccggcca gggcgccgag atcgccgatg tggatggcag
     1081 cccgttccat ctccagcgca atcgaacgga tcgtcagtgc tcgcagggac gggacacagt
     1141 tccccaaaac ctccatggcg tttgccgttg ccgtcgcatg ggcgatgaca ctgtctccgg
     1201 cgatcgattc cgaaacacga accgcctggt ccgccttccg cacaaggagg tcctcgacac
     1261 cccgatgctg atacccgagg gcgatttcaa gatgcaatat ctcctcaccc cggcaatgaa
     1321 aacggaagtg tcccggctcg atgatcccgg catggaccgg cccaacgcca acctggtgga
     1381 cgctgtcgcc ctggaccgga aaaaacgggt agcgttcgac ggcactgcgt cttttcggag
     1441 aacacgctcc agcctgcgga tctccggctt ttctcgtgat gccctgtccg tccacaaacc
     1501 agccgccgcc gcgggcgcgc accggcttga gccaggggtg atggcgggga acgatcccga
     1561 actcctcata cagctcgcgc tcaaaaatgt gcagagccgg cacctcgcgg accatggtcg
     1621 gatactccga accaggctgc accatgcagc ttgcgaccag gagctcggcc cggctgtccc
     1681 gtgcgaggac gcaaaccagc tcgatgcctt cccatcgccg tacgccgaac aaggtgaccg
     1741 gtctcgcccg gtcccggtcg ctcacggagc agagtgaaat gatgtcttcc ctgaatctgg
     1801 aaagcgggaa tcgtgggatg tcatggatgg aaaccggtcg caggttccgg gtacgggacc
     1861 aaccgctgtt catcgctgcc ctccgttcac accgaggatg gccgccacat cgcggaaaaa
     1921 ggtttcaacc ggctgcggca tggccacaac gatggcaatc gccagtccca ggaggagtac
     1981 ctgcggccag atggccgaac tcctgtgtcc ggccagatcc ggccgggccg gccccatggc
     2041 caccgcgagg acgccccggc ccattcccca gatgatgact gtcaggagga gcaaaaagag
     2101 cgcgaggagg acaggatacc ccgcctcaag cagagccgac atgagcatga tctcgctgaa
     2161 aaaagccgcg aagggcggca aacccgagat tgccacaaac gcggtcaacc agacccatcc
     2221 ggttcggggc caggtcgcca gcacgccgcc ggcaccggcg atatcacgcc gcttccaggc
     2281 atggaggatg tctcccgcgg ttaaaaagaa ggcgcccttg acgagagaat gcgccaccat
     2341 gtggagtacg gccgcgacgg cgccggctcc acccaatccg aaaccgatcg ccatgatccc
     2401 catgttttcg atggaggaat aggccagcat ccgtttgtag ttttccaccc ggagcatata
     2461 gaccgccgcg acaaacaggg agaggaagcc cgtcacgacc agggcatccc ggacaaagac
     2521 gccgtatccg gccgcgtcga ggatattccc gaacctgagt acggccagga gtgccgtatt
     2581 gaggagggtc gccgaaagga gtgccgagat tcccgaaggg gcctcggagt gggcatcagg
     2641 aagccaggca tggaccggag cgaacccggc cttggttcca aacccgacaa gaatgaaagg
     2701 aaaagcgaat ttgagccaga aaggatcgat cttccccgcg ttggcctcaa gatccctgaa
     2761 atgcagagat gcccccggct ccatccccat catcaggagg atgatcccga taaaggccag
     2821 agcaatcccg atggaacaga tgaagatgta tttccaggtc gcctcaagcg ctgtctttgt
     2881 ccgtgaggag tagatcaggg gagccgaagc gagggtcgtt ccttcgacaa aaacccagag
     2941 catggccgcg tggttcgtga gagccgctgc ggtcatcgca ccaatgaaaa tcatcaatgc
     3001 cgtggtctgg agtgagtgcc atgcctcatg gccgtcgtgg tgcatccccc gttcatccag
     3061 aaaccgtttg acggccaccg cgagatagac gatggccaga agtgcgagga caagaacggc
     3121 tggcgggtcc gtctcgaaat atccggccca ggaacagggc tcaagggcca tccaggccac
     3181 cccggccaga tgccccatgg catgcagaag gaccagatac ctgtcaaggg ccggacggcg
     3241 catcagaaac gagaggacag cgagagttaa tggaagaaaa aaaagaacag gaattaccat
     3301 gatcagtcct tcaggtccga gagttcatcc acactcatct cctcgtagct cgatgcgatc
     3361 ctgttcatga agagcccaag aagaaaaact gccagaaaaa catcaagcag caatccgacg
     3421 ttgacggcga aggtatgctc gcgggcgaca gccattgtca gcatgaatac tccgttttcc
     3481 atcgccagga atccaagaac atgagtaatg agttttttgc gggagatgat gagaaagagg
     3541 gccgtcacaa tcaccgccag cgaaaccccg agtgccagcg gttttgtccc gctcgtcact
     3601 ccgcgagaga gatgttcgag gagaaacccg ccaccgagaa taatgctggc caccagcagg
     3661 gaataaaaat tcgggatata tggctcgatc tcgcggtaga tatggttgcg gacaatgatc
     3721 cgttgcagga agagggggat caccacggct ttgacgacca gggtctcgat gatcaggaaa
     3781 acaagattga ttgtgtcaaa cgcgtgggcg tcatcgagga ccaggacaaa gagcagcaag
     3841 ccctgtatcg agagggccct gatcaccgca tgg
//
LOCUS       DAOSHE010000177         3867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21560_length_3867_cov_5.741868, whole genome shotgun sequence.
ACCESSION   DAOSHE010000177 DAOSHE010000000
VERSION     DAOSHE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21560_length_3867_cov_5.741868"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="PLD82_05875"
     CDS             complement(<1..2029)
                     /locus_tag="PLD82_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64948.1"
                     /translation="MMRFGLRWLSGLFVLLALFLPFAASAEDVQVRSFGKIRITGSGQ
                     NWTADLGKLGTHRFTGTISPLKLKKNLVATDYEKLPGKAVLKRLGLQEAQLTISSEGL
                     GISARVDTKRNLNGMAKLFKIEAPFIDISARIAPAGLSLRGDLNFSRKSIGLVLIPGL
                     GTRLELDSLSLIFDIGTGLPDMDAPLVQKMTTIDLEIAPVISVRAEARLQPSKWDPLL
                     KTVTQFSYNLVTQEMTLAGSIMDRWVNPLGMSLIFKNREVFVFENAAMEIGWIPGSPV
                     PTKIGYGVEHAKVFGLRFGSVVSIAPTEGQIALKYHLDRLSFNDLANVITKGFGKKMP
                     NLIPGDLGLRNVQVLFSPNGGSVGELQIPQGFILRGEANIGQVLSGTIQFSADFEDGI
                     DLTIELKDSLREHILKKLKNRKVLKAIVGKLTSTLDIRRIAISFKAKRSELGGGIACD
                     MMIFGRNLSFNYSGSINPEALAKEIVDRIVHFCQHEVKEAFVKVGKAFVKAGDVARQW
                     AVKAGARVRAFAVNLGKRMKHSGHSYNKCMGTCVPDYAGGVRRDILDGSNDAVNEFYR
                     LALENITEVEGDTEEETRKLRQDLLADEWKKLCDSIDSEWNSVRGDNEVRKFFTKAKS
                     KDEGVLKYQLLIDETWGAHKTMRQAAYERLMTVDDSVNVSVTVFRKGTGNVT"
     gene            2323..2964
                     /gene="udk"
                     /locus_tag="PLD82_05880"
     CDS             2323..2964
                     /gene="udk"
                     /locus_tag="PLD82_05880"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG64949.1"
                     /translation="MIIIGIAGGTASGKTTVVRSIYENLPSDRVALIPQDAYYKDMGH
                     LPMEERLKVNYDHPEAFEFDLLLEHICALKNGIPVEVPVYDYVTCTRTTESRTVNPQE
                     VIIIEGIMIMTDPKLRDQMDIKIFVDADADDRLMRRIKRDVRERGRTVESVLDAYETR
                     VKPMHNLFIEPSKRYADIIVPQGGSNHVAIDVLTSMIHAKLIVSPAVQGTSTF"
     gene            complement(3063..>3867)
                     /locus_tag="PLD82_05885"
     CDS             complement(3063..>3867)
                     /locus_tag="PLD82_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG64950.1"
                     /translation="RTMSVSLRPRELEMVEIFALHASVALHNARLYQTIRKRNDELLR
                     LINLKETLAQCVTEEIQGPVVAVDKALADLEARFPMREELGRIRQSSSRAVRLVGRLC
                     DMKALEEEAAQIFADSINFSELVRTILDRHADKVEERNLRVRVSISENCNGYRANGHI
                     MRSIFDEVISNAIYYNTNSGMVTIQGRRTREQLVFVITDTGVGIDAKDLERIFEQFAR
                     TENSMELNPAGAGLGLFMVRIFLKYYGGDIQVKSTPNEGTRFTLTMLRH"
BASE COUNT          937 a         1142 c          892 g          896 t
ORIGIN      
        1 ccgtcacatt gcccgtccct ttgcggaaaa ccgtcacgga gacattgacc gagtcatcaa
       61 cggtcatcag cctctcatag gccgcctggc gcatggtctt gtgcgcaccc caggtctcat
      121 cgatcaacaa ttggtatttc agaacacctt cgtccttgga cttcgccttc gtgaaaaatt
      181 tccggacttc attgtcgcca cggacggaat tccattcact gtcgatactg tcacacagtt
      241 ttttccattc gtctgcaagc aaatcctggc gcaacttcct cgtttcctct tcggtatcac
      301 cctcgacctc ggtaatgttt tcaagcgcaa gacgatagaa ttcgttgaca gcatcattgg
      361 atccgtcaag aatgtccctg cggacgccac cagcgtaatc aggaacgcag gtccccatac
      421 atttgttgta cgaatgcccg gaatgcttca tccgtttccc gagattgacg gcaaaagccc
      481 gaacacgagc ccctgccttg accgcccact gacgtgcaac atcccccgcc ttgacaaaag
      541 ccttcccgac cttgacaaag gcctccttga cttcatgctg gcagaaatga acgatccggt
      601 cgacaatctc cttggcaagg gcttcggggt tgatggaacc ggagtaattg aagctgagat
      661 tccgtccaaa aatcatcata tcgcaggcaa tcccgccccc aagctccgaa cgcttggctt
      721 tgaagctgat ggcgatccga cggatatcga gtgtggatgt gagtttgcca acaatggctt
      781 tgagcacctt cctgtttttg agctttttaa gaatatgctc ccgcaatgag tctttcagct
      841 cgatcgtcag atcaatcccg tcctcaaaat cggctgaaaa ctggatcgtc ccggacagga
      901 cctgtccgat attggcttca ccacgaagaa tgaacccctg cggaatctgc aattccccca
      961 cgctgccccc gttgggggaa aacaaaacct ggacattccg caacccgagg tcgcccggaa
     1021 tcaggttcgg catttttttc ccgaaaccct tggtgatgac gttggccaga tcattgaaac
     1081 tcaacctgtc cagatgatat ttcagggcaa tctggccctc tgtgggtgcg atactgacca
     1141 cggagccgaa ccgcaacccg aataccttcg catgctctac gccatatccg atctttgtcg
     1201 gcaccggcga gccaggaatc cagccgatct ccatggcggc attttcaaaa acaaaaactt
     1261 ccctgttttt gaaaatcagg gacattccca gcgggttgac ccagcggtcc atgatcgaac
     1321 cggccagtgt catctcctgt gtgaccaagt tgtacgaaaa ctgcgtaacg gttttgagaa
     1381 gcggatccca cttactcggc tgcaggcggg cttcagccct gacactgatg accggagcaa
     1441 tctccagatc gatcgttgtc attttttgca caagaggcgc atccatgtcc ggcaggccgg
     1501 tcccgatgtc aaaaatcaga ctcagggaat ccagttccag ccgtgtcccc aatccgggaa
     1561 tcaggacaag cccgatcgac tttctcgaaa aattcagatc tccccgcagg gaaagccctg
     1621 ccggagcgat ccgtgcggaa atatcgataa atggcgcctc aatcttgaac aactttgcca
     1681 tcccgttgag gttccgtttt gtgtcgactc gtgccgaaat gcccaatcct tcggaagaga
     1741 tggtaagttg tgcttcctgc agcccaagtc gcttgagaac agccttgccc ggaagcttct
     1801 cgtaatccgt tgctaccaga ttctttttca gtttgagcgg cgaaatcgtc ccggtgaatc
     1861 tgtgggttcc caacttgccg agatcagcgg tccagttctg cccgctccct gtaatcctga
     1921 ttttcccgaa actccggacc tgaacatcct cggccgaagc cgcaaacggc agaaacagcg
     1981 ccaataatac aaacaaacct gacaaccatc gaagtccaaa acgcatcatt ctcctccagc
     2041 ttgtgctatc cagattgtat gccgcctgat gattcatcat attctgttat cgttgtgcaa
     2101 gcggccaaca ggatacaaca gcactcatca gaagagcaca tgaagacagt tacacttgca
     2161 ttccggaata ctcacggaaa catgtccctt tttgaagaaa attattgctc ctatacacat
     2221 atatttgtac gaaattacgc ttctaccttc ttctccggat tccccgctgg acaggtctag
     2281 ccgtatatta ttcccatccc atcgtatcca gggaggatgg ccatgatcat catcggaatc
     2341 gcgggcggaa ccgcatcggg caaaacaaca gttgttcgca gcatctacga aaacctgccg
     2401 tcagaccggg ttgccttgat cccccaggac gcctactaca aggacatggg acaccttcca
     2461 atggaagaac gcctcaaggt caattacgat catcccgagg ctttcgagtt tgacctcctc
     2521 ctggagcata tctgcgcact caaaaacggc atccccgtgg aagtcccggt ctacgactat
     2581 gttacttgta ccagaacaac cgaatcccgg accgtcaatc cccaggaagt gatcatcatc
     2641 gaaggaatca tgatcatgac agacccgaaa ctccgggacc agatggatat caagatattc
     2701 gtggatgccg acgcggacga ccgcctgatg cgccggatca aacgtgatgt ccgggaacgg
     2761 ggccgaactg tcgaatccgt cctggatgcc tatgagaccc gtgtcaaacc gatgcacaac
     2821 ctctttattg aaccgtcaaa gcgctatgcc gacatcatcg ttccccaagg tggcagcaac
     2881 catgttgcta tcgatgtcct gacctcgatg atccatgcca agctgatcgt ctcaccggca
     2941 gttcaaggca ccagcacctt ctgatttgtt tccgtcatcg tgtgatattt tttgctgaac
     3001 agcctcaggc cagtcactcg cgattcggat ccaggccaag ctccgctggc cggaatacac
     3061 gattaatgcc tgagcatcgt caacgtaaaa cgcgtcccct cgttgggtgt gcttttcacc
     3121 tggatatccc cgccataata cttgagaaaa attcgcacca taaagagtcc aagaccagca
     3181 ccggccggat tgagctccat ggaattttca gtccgtgcaa actgttcgaa tattctttcg
     3241 agatcctttg catcaatccc gaccccggta tccgttatca caaaaacaag ctgctcacgg
     3301 gtccgccggc cctggatggt gaccatccca ctattcgtgt tgtagtagat ggcattgctg
     3361 atgacttcat caaaaatcga acgcatgatg tgcccgtttg cccggtatcc gttgcagttt
     3421 tccgagatgg atacacgcac cctgagattc cgctcctcca ccttgtcagc atgccggtca
     3481 agaatcgtcc tgacaagttc ggagaaattg atcgaatcgg caaaaatctg ggcggcctcc
     3541 tcctcgagcg ccttcatgtc acacaacctg cccacaagac ggaccgcgcg cgagcttgat
     3601 tgccggatcc ggccaagctc ctcccgcatc ggaaaccggg cctcaagatc ggccaaagcc
     3661 ttgtcaacgg caacaactgg cccctgaatc tcctcggtga cacactgcgc aagggtttcc
     3721 ttgagattga tcaggcgcaa cagctcgtca ttcctctttc gaatagtctg gtacagccgt
     3781 gcattatgga gagcaacact ggcatgcagg gcgaatatct cgaccatctc gagctcacgg
     3841 ggcctgagag agacagacat cgtccgg
//
LOCUS       DAOSHE010000178         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21594_length_3864_cov_4.123917, whole genome shotgun sequence.
ACCESSION   DAOSHE010000178 DAOSHE010000000
VERSION     DAOSHE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21594_length_3864_cov_4.123917"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="PLD82_05890"
     CDS             <1..2190
                     /locus_tag="PLD82_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64951.1"
                     /translation="AGGREVSAVAKKKTETAAVSRKRGTGVTQPEVSTTKPVEERPKI
                     VDTKRSLLALGFLVLAALLALLWRQGNADVNALIDAAREDMKNGRYEQAIGRLDDAAR
                     ALADLRKQLEEKSSGPDDEVRLRGMDELSRRLDEMRREAQQGKDDSAKGGDPAARVRR
                     LMRRAEDDEKIGLSQRALDKLDLALDILDAPGGSGLRDADAERFRQDIAARRTRLADR
                     IKADDSSKRTDLRGRDDRPDRDEVRLSPRDRELLRRLEADTRKDDKKATSDDPRDAAR
                     QLVREGRQQLLQNRNNPARDTFSKALDKDERNPDAWSGRSTANLRLSRMGDALSDAER
                     ALALSPTDAGAHMTKAEVAFSRDQLDSALDSYRKVVQYDPENMMAWYRLGYIYYRRGA
                     YQLARDAFARTVAIDRNFAKAWFNLGLSHEQLGNAASAITAYTRAMQSQSGYDKPAFN
                     LGRLYYKAGNYPAAETQFLAVLKNQPDNGDALVYLGLVSDGRGRYQAAAGYYRRALDR
                     DPKNYVAAYNLGRMHEKLGEWTPAVDAYKKALDIRPGSAEASHNLGTTFIKANRLREA
                     LDYFGKAHSANPGDIETNIALSKVYRLLGQPEKALQHVQKAIASDGNNARAHASKGLI
                     LAGQEMYDEAIAAYRTALGIQPADGDARFNLGEALRLSKRYDDAISEFGKLLQFEPGN
                     GLARQRIGEIQMLQGKNAEAKATFTRLLSEQPGYAQRAVVEGHLKKL"
     gene            2202..3371
                     /gene="dinB"
                     /locus_tag="PLD82_05895"
     CDS             2202..3371
                     /gene="dinB"
                     /locus_tag="PLD82_05895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOG64952.1"
                     /translation="MEPEETRGQTRIVIHVDMDAFFAAVEQRDDPALRGKPVIVGGPP
                     DSRGVVCTCSYEARAFGVHSAMPCSLAWRLCPQAIFVKPRFAAYELVSRHIRRIFFRY
                     TDCIEPLSLDEAFLDLGEVHCMPAARVLFPESGAVEDVFVWGTMIGQKIRGEILRETG
                     LTASAGIAANKFLAKLASDMDKPDGLTVLSPERVAGVLAALPIERFWGVGRVTASRLR
                     ARGITDGASLLARSQEELVRQYGKGGLVLWEMARGIDRRPVEAERERKSYGRETTFQR
                     DIENLEEIQDVLASLAGEVSAGMIAAGVSGTTITVKVRTADFVTVTRSHTMPRPTADA
                     GTIWHEALALLSKTRAGLDPLRLVGVSVSGLDHKDQESLQPDLPFPPPWKPLRES"
     gene            3422..>3864
                     /locus_tag="PLD82_05900"
     CDS             3422..>3864
                     /locus_tag="PLD82_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG64953.1"
                     /translation="MVDQEALVRDAVALVRTPSLSGQERAAARLFGSQLEALGFDEVR
                     TDASGNVIGIIRGGRPGGRIMFEGHIDTVPEGDVSLWTREPYGGEVSGGRIWGRGTSD
                     MKGALVAMAHGLANVIPHKTGLSGELVLTAVVCEEIFEGIAFGQV"
BASE COUNT          775 a         1110 c         1255 g          724 t
ORIGIN      
        1 gctggggggc gagaagtatc cgccgtggcg aaaaagaaga cagagacggc tgctgtttcc
       61 cgaaaacggg ggacaggggt gacgcagcca gaggtatcta cgacaaaacc tgtggaggag
      121 aggccgaaaa tcgttgatac aaaacgcagc ctccttgcgc tgggatttct cgtcctggcc
      181 gcccttttgg ctctgctttg gcggcaggga aatgcggatg tcaacgctct gatcgatgcc
      241 gcccgggagg acatgaagaa cggtcgctat gaacaggcca tcggtcggtt ggacgatgcg
      301 gcccgggcgc tggctgatct tcggaagcag cttgaggaga aatcgtccgg accggatgac
      361 gaggtccgac tcagggggat ggacgagctg tcccgccggt tggacgagat gcgtcgcgag
      421 gcccaacaag gcaaggatga ttccgccaaa ggcggcgatc cggctgcaag ggtccgccgg
      481 ttgatgcgca gggcggagga tgatgaaaag atcgggctgt cccagcgtgc tctcgacaag
      541 ctggatctgg ccctggatat tctggatgct cctggcggga gcggtcttcg cgatgcggat
      601 gccgaaagat tccggcagga tatcgccgcg cgccggaccc ggttggccga ccggatcaag
      661 gccgacgatt catcaaagcg gaccgatctt cgcggacggg atgaccggcc tgaccgtgac
      721 gaagtccggc tttctccccg tgacagggaa ctcctccgtc gccttgaagc cgatacccgc
      781 aaggacgata aaaaggccac aagtgatgat cctcgtgatg cagcccgaca actggtacgc
      841 gaagggcggc agcaactctt gcaaaaccgg aacaatcctg cccgggatac gttttcgaaa
      901 gcgctggaca aggacgagag gaatcccgat gcctggtccg gaagaagcac ggccaatctc
      961 cgcctgagcc ggatgggcga tgccttgtcc gatgccgaac gggcgctggc actctcgcca
     1021 acggatgccg gggcccacat gacaaaggcc gaggtggcct tctccaggga ccagttggac
     1081 agtgccctcg actcgtaccg caaggttgtc cagtacgacc ccgaaaacat gatggcttgg
     1141 taccgtctcg gatacatcta ctaccgcagg ggggcctacc agttggcccg tgatgctttt
     1201 gcccggaccg ttgccatcga ccggaatttt gccaaggcct ggttcaacct cggattgtcc
     1261 cacgagcaat tgggaaatgc tgcgtcggcc attacggcgt acactcgggc catgcagtcg
     1321 caaagcgggt acgacaaacc cgccttcaat ctcggtcgtc tctattacaa ggccgggaat
     1381 tatccggctg cggagaccca gttcctggcc gtgctcaaaa accagcctga caacggtgac
     1441 gcccttgtct acctcgggct ggtctctgat ggacgcgggc gctaccaggc tgcagccggc
     1501 tactatcgtc gagccctgga ccgtgatccg aaaaactatg ttgcggcata caacctcggc
     1561 cggatgcatg agaagctcgg cgagtggact cctgccgtag atgcctataa aaaggcgctg
     1621 gacatccggc cggggagcgc cgaggccagc cataatctcg ggacaacctt tatcaaggcc
     1681 aatcggctcc gggaggctct cgattatttc ggcaaggcac actcggccaa tccgggggac
     1741 atcgagacca atatcgcgct ctccaaagtc taccgcttgc tcggtcagcc tgaaaaggcc
     1801 ctccagcatg tccaaaaagc gattgccagt gacgggaaca atgcccgggc ccatgccagc
     1861 aaagggctga tcctggccgg gcaggagatg tatgacgaag cgatcgccgc ctaccggacg
     1921 gcactcggga tccagcccgc cgacggggat gccaggttca atcttggcga ggcgctccgt
     1981 ctttcgaagc ggtatgatga cgcgatcagc gagttcggca aactcctcca gtttgagccg
     2041 gggaacggcc tggccagaca gcggatcggg gagatccaga tgctccaggg gaaaaacgcc
     2101 gaagccaagg ccaccttcac ccgattgctg agcgaacagc ctggatacgc ccaacgggcg
     2161 gttgttgagg gacacctcaa gaaactctga ggttgtgcag gatggagccg gaagagacac
     2221 gcgggcagac ccggatcgtg atccatgtgg atatggacgc cttttttgcc gcggtcgagc
     2281 aacgggatga tccggctctc agggggaagc ccgtcattgt cggcgggcct cctgattccc
     2341 ggggcgttgt ctgtacctgt tcgtatgaag cccgggcctt cggggtgcat tcggccatgc
     2401 cctgcagcct ggcttggcgg ctttgtccgc aggccatttt tgtcaaaccg cgttttgccg
     2461 cctacgagct ggtttcgcgc cacatccggc gcattttttt ccgctatacc gactgcatcg
     2521 agccactgtc cctggatgaa gcctttctcg atctggggga ggtgcattgc atgccggcag
     2581 cgcgggttct gttcccggaa tccggggctg tcgaagatgt ttttgtatgg ggaacgatga
     2641 tcggccaaaa aattcgcggc gagatcctcc gtgaaaccgg gctgaccgcc tccgccggaa
     2701 ttgccgccaa taaatttctg gcaaagctcg cctccgatat ggacaagccc gacggattaa
     2761 ccgtcctctc cccggaaagg gtggccggcg tgctggcagc cctcccgatt gaacggtttt
     2821 ggggcgtggg acgggtcacg gccagtcgct tgcgggcccg aggcatcacg gatggcgcca
     2881 gtcttctcgc gcgcagccag gaggaactcg ttcgccagta cggcaagggc ggattggttc
     2941 tgtgggagat ggcgcgcggg atcgaccgcc ggccggttga agcggagcgg gagcgcaagt
     3001 cctatggacg cgagacgaca tttcaacggg atatcgaaaa tctcgaagag atccaggacg
     3061 tgctggcgtc gctggccggc gaggtctcgg ccggaatgat cgcggccgga gtgtctggaa
     3121 caacgatcac cgtcaaggtc aggacggcgg attttgtcac ggtgacccgg tcgcatacca
     3181 tgccccggcc gacggctgat gcagggacca tctggcatga agccctggcc cttctctcaa
     3241 agacccgggc cggactggat cccctccgcc tggtcggcgt ctcggtctca gggcttgatc
     3301 acaaggacca ggagagtctt cagcccgacc tcccattccc gcccccatgg aagcctctcc
     3361 gcgaatcctg aaaatggcta ccttgttggg agaaggacgt tttcaggttc tggaggttgt
     3421 catggttgat caggaggctt tggtccgcga tgctgtcgca ctcgtgcgga cccccagtct
     3481 gtccgggcag gaaagggcag cggcccggct ttttggcagc cagctcgaag ccctcgggtt
     3541 tgacgaagtg cgcacggatg catcagggaa tgtgatcgga atcatccggg gcgggcgtcc
     3601 gggcgggcgc atcatgtttg aggggcatat cgacacggtc cccgaggggg atgtgtccct
     3661 ctggaccagg gagccctatg ggggcgaggt ttctggaggc cggatctggg gccgggggac
     3721 gagcgacatg aagggggccc ttgtcgccat ggcccatggc ttggcgaacg tcattcctca
     3781 taaaaccggt ctctccgggg aactcgtcct gacagcggtt gtctgtgagg aaatttttga
     3841 agggatcgct ttcggacagg tcat
//
LOCUS       DAOSHE010000179         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21663_length_3858_cov_3.596371, whole genome shotgun sequence.
ACCESSION   DAOSHE010000179 DAOSHE010000000
VERSION     DAOSHE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21663_length_3858_cov_3.596371"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..986)
                     /locus_tag="PLD82_05905"
     CDS             complement(117..986)
                     /locus_tag="PLD82_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG64954.1"
                     /translation="MPLWLAASLLAMLVFTIYGLFQKALVMRHPEHPVAIAAMTKFLG
                     TFFIALSLPLEPARLPAEPGPWLLLALNILVWTCANILDIRSFRLTEVSDIYLIRSLS
                     PALSFCSGIILFGEPFALTKLAGLVLILGGLVVLSYRKGSLVFDQGQRLQLVTTILLP
                     IGVGLDMYLLRHFSLLFYITVNLGMLPLPMILLAGSERQRIGREFLAMFRRPVLMTGP
                     AFLGFCLAANALLLMLAMKHGELSRIMPLMTGSTLLIVAGGWLFLGERDRLARKLTTT
                     IMGIAGVLLLTLS"
     gene            complement(1549..3000)
                     /gene="gnd"
                     /locus_tag="PLD82_05910"
     CDS             complement(1549..3000)
                     /gene="gnd"
                     /locus_tag="PLD82_05910"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOG64955.1"
                     /translation="MDKADIGLIGLAVMGENLALNLESRGYRVAVYNRSADKVTRFVQ
                     GRANGKNFLGATKIEELVFSLKTPRKIIMMVKAGDVVDELIGSLVPHLEQGDILMDGG
                     NSHYNDTERRWKALSEKGIRYIGTGISGGELGALTGPSIMPGGDPAAWPEVQPLLQSI
                     AARAGKDEACCEWIGQGGAGHFVKMVHNGIEYGDMQLITEAYQIMHQGLGMNHAEMAT
                     VFGQWNDGELGSYLIEITRDIMGYLDEDGTPLVEKILDVSGQKGTGKWTGITALDLGV
                     PLTLIGEAVFARCLSSVKDERVAASQVLDGPDKRITNPGENFISALRDAVYAAKIISY
                     AQGYALMREAAADHGWDLNYGGIAAIWRGGCIIRSVFLDRIKEAYETTPGLTNLMLAP
                     FFRETLARAQAGWREVVAQAARVGIPVPAISSALAYYDGYRCANLPANLLQAQRDYFG
                     AHTYERVDKPRGEYFHTNWTGKGGTTASTTYQR"
     gene            complement(3260..>3858)
                     /locus_tag="PLD82_05915"
     CDS             complement(3260..>3858)
                     /locus_tag="PLD82_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HOG64956.1"
                     /translation="NFGLSFGLVPLGDQLGGLMPAAFSTITGGMPPVEMGPLYGSLGG
                     KLVAIAFAFVLGFGATIAEPALNAMGIQVENLTQGAFKRSGLIITVAAGVGFGIAIGV
                     ATFVFSVNFLYILLPAYCVILFMTKMSSEIMVNIGWDSAGVTTGPITVPLVIALGLGI
                     GGNIPTVVDGFGILALASAFPVLSVLGMGLIVKRRAAG"
BASE COUNT          841 a         1090 c         1160 g          767 t
ORIGIN      
        1 ccgttctccg cagcccgcgc gaggggagag acctctcccc tgccaaaaaa tttttgttag
       61 attgcgatgc ctgagacgcg atgaatcgca tcgcttcaca agacagaacg tagttgtcag
      121 gagagggtta acaggaggac accggcgatg cccatgatcg tggtcgtcag ttttcgggcc
      181 agtcggtccc gttcaccgag aaagagccag cctccggcca caatcaggag ggtactgccg
      241 gtcatcagcg gcatgatccg tgagagctct ccgtgtttca tggccagcat caggagcaac
      301 gcgttggcgg ccaggcagaa gccgagaaag gcgggtcctg tcatgaggac cggtctgcgg
      361 aacatggcca aaaattccct cccgatccgc tgtcgctcgc tgccggccaa gaggatcatc
      421 ggcagcggca gcatcccgag gttgacggtt atatagaaga ggagtgagaa gtggcgcagg
      481 aggtacatgt cgaggccgac tccgatcggc aggaggatgg tcgtcacaag ctggagtcgt
      541 tggccctggt caaacacgag gctccccttg cggtacgaga gaacaaccag tccgcccagg
      601 atcaggacga ggccggccag cttggtcagg gcgaagggtt ccccgaagag gatgatcccc
      661 gaacagaagc tgagggccgg tgagaggctc cggatcaggt agatgtcgga gacttcggtc
      721 aggcggaagc tgcggatgtc gaggatgttg gcgcaggtcc agaccaggat gttcagcgca
      781 aggagaagcc aggggccggg ttccgcgggc agtctggccg gttcgagtgg cagggagagg
      841 gcgataaaga aggtcccgag gaattttgtc atggccgcaa tcgccaccgg gtgttcgggg
      901 tggcgcatga ccagggcctt ttggaaaaga ccgtagatgg tgaagacgag catggcgagg
      961 agagacgccg cgagccagag aggcatattg acaaccttgt caccacagga tgtccggtaa
     1021 aggtacttct ccgacacccc gttcggacgg aattggcatg gcgcttttgg gacaatgcga
     1081 tccgggccat cccgatccga tgcgccgcct tgtgtacgtt atcgtgattg tgttatgcga
     1141 atcgggatac gcatgacagt attgtggatt ggcgtaagca atcccggata cgcatgacga
     1201 tattgtggtt ttgtacggtg taaacggcaa gcgtgatggg gatggttggc ccgagacgcg
     1261 atgaatcgcg tctctacaaa ggacggcata cgtgatagat gcggcttcat attgcgcctg
     1321 caccagagag gggagcgcgg gatacgggag tcgcgtttct ccaataatat aatacaggcc
     1381 gggcccataa gaggattccg ccctccgcag cccgcgcgag gggagagacc tctcccctgc
     1441 caaaaaatct ccctgaatgc gatacctgag acgcgatgaa tcgcgtctct acgaagacgg
     1501 catatcgtga ttgttgcgcc atgacgaaga cgggcaatct ggatcggatc aacgttggta
     1561 ggtcgtgctg gcggttgtcc cgcccttgcc ggtccagttg gtatggaagt actcgccccg
     1621 tggtttgtcc acccgttcgt acgtatgcgc gccgaagtaa tcacgctgtg cctggaggag
     1681 gtttgcgggg aggtttgcac agcggtagcc gtcgtagtag gccagcgcgg aactgatggc
     1741 gggaacgggg atgccgacac gtgccgcttg cgccacgact tcgcgccagc cagcttgcgc
     1801 ccgggccagg gtctcgcgga aaaacggcgc cagcatcagg ttggtcaggc cgggcgtggt
     1861 ctcataggct tccttgatcc ggtccaaaaa cacggatcgg atgatgcagc caccacgcca
     1921 gatcgccgcg atcccaccgt agttcaggtc ccagccgtga tcggccgcag cctcacgcat
     1981 cagggcatac ccttgggcgt acgaaatgat cttggcggca tagaccgcat ccctgagtgc
     2041 gctgatgaag ttctcccctg gattcgtgat ccgtttgtcc ggtccgtcga ggacttgtga
     2101 tgcggcgacc cgttcgtcct tgacggacga gagacagcga gcgaagacgg cttctccaat
     2161 cagggtcagg ggcacgccga gatcgagagc cgtgatccct gtccattttc ccgttccttt
     2221 ttgtccggag acgtccagga tcttctcgac cagcggggtt ccatcctcat cgaggtagcc
     2281 catgatgtca cgggtaatct cgatcaggta cgagccgagt tccccgtcgt tccattgtcc
     2341 aaatacggtg gccatttcgg catggttcat ccccagccct tggtgcatga tctggtaggc
     2401 ttcggtaatg agctgcatat caccgtactc gatcccgttg tgcaccatct tcacaaaatg
     2461 gccggcgccc ccctgcccga tccactcgca gcaggcttca tccttgccgg cccgggccgc
     2521 aatcgactgg aggagaggct ggacttcggg ccaggcagcg ggatccccgc cgggcatgat
     2581 cgagggtccg gtcagcgctc cgagttcccc acctgaaatc cctgttccga tataccggat
     2641 ccctttttcc gagagagcct tccagcgtcg ctcggtatca ttgtaatggg aatttccgcc
     2701 gtccatcagg atgtcgccct gttccagatg cggcacgagg ctcccgatca gctcatccac
     2761 cacatcgccg gccttgacca tcatgatgat cttgcgcggg gtcttgaggg agaagacaag
     2821 ttcttctatc ttcgttgcgc cgagaaagtt ctttccgttg gcccggcctt ggacaaaacg
     2881 ggtcaccttg tccgcgctcc ggttgtacac cgccacccga tacccgcggc tctcgagatt
     2941 gagggccaga ttctccccca tgactgccag tccgatcaat ccgatatcgg ccttgtccat
     3001 cgtttgcctc cccttgttgc acagcatttc cgttctccat gcgcctcttg tatcatagat
     3061 atatttatat gttatgtcca gagaaaaaaa acggggggat ttgcgtcggg aatcccgtat
     3121 tcgcatgact ttttcaaggt ttgtacccca agacgcgatg aatcgcgtct ctacaaaggc
     3181 ggcaagcgtt gatggttttg tatggcgaaa tcggcaagcg tgatggggat gattatccaa
     3241 aataaaaagc aaaaccacat cagccagcag cacgccgttt gacgatcagg cccatcccca
     3301 ggaccgagag gaccgggaag gcgcttgcca gggccaatat gccaaagcca tcaacgacag
     3361 tcggaatatt gccgccgatc ccgaggccga gcgcaatgac caagggcaca gtaatcggtc
     3421 ccgtcgtaac acccgccgag tcccacccga tattgaccat gatctcgctg gacattttgg
     3481 tcataaaaag aatcacacag tatgccggca ggaggatgta caggaaattg actgaaaaca
     3541 caaaggttgc cacaccgatg gcgattccaa agccgactcc ggcggcaacg gtgatgatca
     3601 gaccggagcg tttgaacgcg ccctgtgtca ggttttcgac ctggattccc atcgcgttga
     3661 gcgcaggctc ggcaatggtc gcgccaaacc cgagaacaaa ggcaaaggca atcgcgacaa
     3721 gcttgccccc gagggatcca tacaaaggcc ccatctcgac aggaggcatc cctcccgtga
     3781 tggtcgaaaa agcagccggc atgagcccgc ccaactggtc acccagggga accagcccga
     3841 aggaaagccc gaagttga
//
LOCUS       DAOSHE010000180         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21678_length_3856_cov_4.003420, whole genome shotgun sequence.
ACCESSION   DAOSHE010000180 DAOSHE010000000
VERSION     DAOSHE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21678_length_3856_cov_4.003420"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLD82_05920"
     CDS             complement(<1..636)
                     /locus_tag="PLD82_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64957.1"
                     /translation="MKIVLISLSILVFLFLPEILSLSATEIDLPNGNQAPSLAHPFGQ
                     DLKGRDLFARVIHGTRLSLMIGLAATLVSCTIGTLWGAVAGFRGGKVDTVMMRIVDVL
                     FGLPFMFVVIILMAFVGRSILLLFLVLGLIHWLTMARVLRAEVTLIKNREFVQAARSL
                     GCSTWRILSRHILPNISDTIIVYMTFTIPGVVLEEAFLSFLGLGVPAPEASL"
     gene            complement(633..1562)
                     /locus_tag="PLD82_05925"
     CDS             complement(633..1562)
                     /locus_tag="PLD82_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64958.1"
                     /translation="MKKRILLRLGSTFLVLACIITASFFLIRLAPGGPFDAERAISPE
                     IRAQLMRQYGLDQPLHVQYLHYIENLLHFDLGMSMKYPGKSVSGILSEGLPWSAGLGA
                     FALLTALFFGISMAWLGVQMPGRNLDRWLTRISLVCLSLPGIIIAPLSVLIFSLWLGL
                     LPAGLLEGPEHLVLPVFALAIPLAARIFLLMRDSIQTQTASTWFKAALGRGITSSTIF
                     LRHVLRHAMAPVAAFLAPASAALLTGSIVIETVFILPGIGRHFIQAALNRDLTLITGT
                     VIVYSAILLFFNLLADLLLMLLDPAKTDLEAGP"
     gene            complement(1559..3166)
                     /locus_tag="PLD82_05930"
     CDS             complement(1559..3166)
                     /locus_tag="PLD82_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG64959.1"
                     /translation="MSRGSRILSAILLLAWMACAPKDEWFGVTEPPRDDTLIVNNGTE
                     PETLDPALMNGNIEGNLAFSLFEGLTRFDPKTLDPLPGVATNWHVSADGRTWTFHLRS
                     DAVWSDGSPITSLDFAWSWLRLLAPTTAARYASFLYLVENAEAFSMGTMTNATRVGIR
                     CPDSQTLVVTLVRPAPYFPFMTAFYVTMPVHRKSVERHGQDWTNPENLVSNGPFVLKT
                     WQMHRKIELIKNERYHGRNEVHLKRVIFLPIEDTGTVLNLYRRGDIDMLNDVPLELFS
                     WLKTRRDYSGHPGWGVYYYKFNINRKPFDDRRVRLALSLAINRSRLTDEFLAQGQIPA
                     TAFTPPIPDRYFPPGKAIYDPQAARKLLADAGYPEGRGFPAVRLLYNTIATDLHRHVA
                     EVLQAMWKQELGITIQLEVQEWKTYLKSVENREFDLARAGWVGDFPDPGTFLDLFLQA
                     DGNNNTGWTDPEYTRHVEAALFDKNPATRMQSFHKAESILLDEMPVIPVFYYAFTMLK
                     KPWVDGVFGNFQDIHPLRSVRIRKAGS"
     gene            complement(3183..>3856)
                     /locus_tag="PLD82_05935"
     CDS             complement(3183..>3856)
                     /locus_tag="PLD82_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64960.1"
                     /translation="QYFDRTAFYHGMPTTAPRYDANKTFIPTKDEREELALVFWRGSM
                     FLFCTQNGNRGWIVNYESLPSRGDAVCIDDDDEVLFLATRHGEILAIDPTTGLLLNMT
                     IESSGTSFRYPAIPVDHGNGQLTLALDARHNILWWTGSGSRYLTRLSWSRSGGQIFAR
                     YDSRIDAHTIPLSDTPFPPTTSMDRLLSPVVLHANGTFNILTSGVKPRYLRFDHQGST
                     SPGPG"
BASE COUNT          893 a          975 c         1204 g          784 t
ORIGIN      
        1 caggctggct tcaggcgcag gaactccgag gccgagaaat gacaggaaag cttcttcaag
       61 gacaaccccg ggtatggtga aagtcatata gacgatgatg gtatcactga tattcggcag
      121 gatatggcgt gacaggattc tccaggtgct acacccaaga gatctggctg cctggacaaa
      181 ttcccggttt ttgatgaggg tcacttcagc cctgaggacc cgggccattg tcaaccagtg
      241 gatcagcccc aggacgagaa ataaaagaag aatcgaccgt ccgacaaatg ccatgaggat
      301 gatgacgaca aacatgaagg gcagaccaaa gagaacgtcc acaatccgca tcatcaccgt
      361 atccactttt cctccgcgaa acccggcaac ggcaccccag agtgttccga ttgtacagct
      421 caccagtgtt gcggccagtc cgatcatcag ggagagtctg gtgccatgga tcactcgggc
      481 aaacagatcc ctgcctttga gatcctgtcc gaaggggtga gccagacttg gcgcttgatt
      541 cccgttcggg aggtctatct cggttgccga gagggagaga atctcgggca aaaacaggaa
      601 cacgagaatg gagagactga tcaggacgat tttcacgggc cagcctccag atccgttttt
      661 gccggatcga ggagcatcag gagcagatcc gccagaaggt tgaagaagag aaggatcgcg
      721 ctgtacacga tgactgttcc ggtgatcagg gtcaaatccc tgttgagtgc cgcctggatg
      781 aaatggcggc cgattccggg aaggatgaaa acggtttcga tgacgatgct gcctgtcaag
      841 agggcggcac tggccggtgc caggaaggcc gcgacaggcg ccatggcatg cctcaggaca
      901 tgacgcagaa aaatggttga cgatgtgatc cctcgaccga gtgccgcttt gaaccaggta
      961 ctggcagtct gggtctggat gctgtcccgc atcagaagga aaatccgtgc tgccagggga
     1021 atggccaggg cgaataccgg aaggacaaga tgttcaggtc cctcgagcag cccggccggg
     1081 aggagtccaa gccagagaga gaagatcagg acggagagtg gagcgatgat gatgcccggc
     1141 agggagaggc agaccagaga gatccgggtc agccagcgat caagatttcg cccgggcatt
     1201 tgcacaccca gccaggccat gctgattccg aaaaacaggg ctgtcagcag tgcgaaggct
     1261 cccagacctg ccgaccaggg gagtccctcc gataaaatac ctgaaacaga ttttccggga
     1321 tatttcatgg acatccccag atcgaaatgc agcaggtttt cgatgtagtg cagatactgg
     1381 acatgcaggg gctggtccag tccgtactgc cgcatgagtt gggcacggat ctccggcgag
     1441 atggcgcgtt cggcatcgaa cggccctccg ggggccagac ggatgaggaa gaaacttgcg
     1501 gtgatgatgc aggccaggac caggaacgtg gaaccgagtc gtaacagaat ccgttttttc
     1561 atgagccggc ctttctgatc cggaccgagc ggagcggatg aatatcctgg aagtttccga
     1621 agacaccgtc tacccagggc ttcttcagca tggtaaaggc gtagtagaaa accggaatga
     1681 ccggcatctc gtcaagaaga atcgattcag ccttgtgaaa ggattgcatc cgtgtcgccg
     1741 ggtttttatc gaaaagggct gcctccacat gccgtgtgta ctccgggtcg gtccagccgg
     1801 tattgttgtt gccgtcagcc tgtaagaaaa gatcgagaaa tgtccccggg tcgggaaagt
     1861 ccccgaccca accggctctt gccagatcga attcccggtt ttcgaccgat ttgagatagg
     1921 ttttccactc ctggacctcg agctggatcg taatcccgag ctcctgtttc cacatggcct
     1981 ggaggacctc cgcgacatgt ctgtggagat ccgtggcgat ggtgttgtag agaagccgga
     2041 ctgccggaaa tcccctgcct tccggatagc cagcatcagc caggagtttg cgggccgctt
     2101 gcggatcgta gatggccttg cccggtggaa aataccggtc agggatcggt ggcgtgaatg
     2161 ccgtcgcagg gatctggcct tgagccagga actcgtcggt cagtctggaa cggttgatgg
     2221 cgagcgagag ggccagtcgt acccggcgat cgtcaaaggg cttgcgattg atattgaatt
     2281 tgtaataata gactccccag ccgggatgac cggaatagtc gcgtctggtc ttgagccagg
     2341 agaagagttc cagggggaca tcattcaaca tgtcgatatc gccccgtctg tagaggttga
     2401 gaaccgtacc ggtatcttcg atgggcagga agatgacccg cttgaggtga acctcgttcc
     2461 ggccatgata gcgttcgttt ttgatcagtt cgatttttcg gtgcatctgc caggtcttga
     2521 ggacgaaagg tccgttcgag acgagatttt ccggattggt ccagtcctga ccatggcgct
     2581 cgacagattt tcggtgaaca ggcattgtga cataaaaggc agtcatgaac gggaaatagg
     2641 gtgccggacg gacgagggtt acgacaagtg tctgcgaatc ggggcatctg atcccgaccc
     2701 gggtggcgtt tgtcattgtt cccatggaaa aggcttcggc gttttccacg aggtagagaa
     2761 aagacgcgta gcgggccgct gttgtcggcg cgagaagccg gagccagctc cacgcgaaat
     2821 caaggcttgt gatcgggctt ccgtcggacc agacggcatc agacctgagg tggaaggtcc
     2881 aggtcctgcc gtcagcggag acatgccagt tcgttgccac accgggcagc gggtcaagag
     2941 tttttggatc gaagcgagtc agcccttcaa agagggaaaa ggccagattc ccctcgatat
     3001 tgccgttcat cagggccgga tccagtgtct cgggctcggt gccgttgttg acaatcagcg
     3061 tatcatcacg aggtggctct gtcacgccaa accattcgtc cttcggggca caggccatcc
     3121 aggcaagaag aagtatggca gacaggatcc tcgatccgcg ggacatggct cctccttgtt
     3181 ggtcagcccg gtccgggtga ggttgaaccc tgatgatcaa agcggagata gcgcggcttg
     3241 acaccggagg tgagaatgtt gaaagtcccg ttggcatgca ggacaacagg ggagagaagg
     3301 cgatccatgc tcgttgtcgg tggaaacgga gtgtccgaga gcgggatggt atgcgcatca
     3361 attctggaat cgtagcgggc aaagatctga cccccgctgc gggaccagga gagtcttgtc
     3421 agatagcggc tcccgcttcc ggtccaccag aggatattgt gccgggcatc aagcgccaat
     3481 gtgagctggc cgtttccgtg atcgacggga atggccgggt agcggaaact cgtaccggag
     3541 gattcgattg tcatattgag gaggagtccg gttgtcggat caatcgccag aatttcgccg
     3601 tgacgggttg ccaaaaagag gacttcgtca tcgtcgtcga tacagaccgc gtccccccgg
     3661 gacggcaagc tttcatagtt gacaatccag ccccggttgc cgttttgtgt acagaacagg
     3721 aacatgcttc cacgccagaa gaccagggcc agttcttccc gttcgtcctt tgtcggaatg
     3781 aaggtcttgt tggcatcgta gcgtggggcg gtggtgggca tgccgtgata gaaggccgtc
     3841 ctgtcaaaat actggt
//
LOCUS       DAOSHE010000181         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21700_length_3854_cov_3.950777, whole genome shotgun sequence.
ACCESSION   DAOSHE010000181 DAOSHE010000000
VERSION     DAOSHE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21700_length_3854_cov_3.950777"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PLD82_05940"
     CDS             complement(<1..2026)
                     /locus_tag="PLD82_05940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOG64961.1"
                     /translation="MQRLNRAGMLAILLCVGFFPNFLQAVDKVVLQLKWRHQFQFAGY
                     YTARELGYYADAGLDVEIRPATPGLDPVDEVASGRAQFGIGSSELIINRYKGKDVVVL
                     AAICQHSPYAVLVPESSPIKTVHELTGKRIMFEPQAAEIEAFLRNEGVNNFFKITHSH
                     NPLDIVTGRADAMTAYVTTEPFFLRHAGVETRRFLPEQGGVDFYNDCLFADGKIVDGN
                     PEMVRRFREASLKGWRYAFERPQQIIDLIKSRYDRGISRAFLEFEMKEMRKLVLPELV
                     DIGHINPGRWRHIADTYAELGLLPKNYSLDGFIWTSDEGDGSSRLQWVFAAGLTALLL
                     ILGIVLPIVRINRRLREGEARRQALFDSSPLPIAWAYPDGRFEFLNRRFITFFGYDLA
                     ETPTIASWAGLVIADASGQEALRLLWEHAKSGSAQSGKQGHSTDLTVRCRDGVVREVV
                     VLTQLAGGRVIIMFDDITERSRAEEALRMRITMLTGDGGSGGVDLASLFDMDELQAIQ
                     DAFSEATGVASLITTPDGIPVTRPGNFCELCQEVIRSTEIGRERCRLSDALVGRYNPD
                     GPNVQPCLSGGLWDAGASITVGGRHIANWLVGQVRCGDLDEGRMMEYAREIGADPERY
                     REAMKRVPIMTEERFRKIADFLFKLAKELSIKAYRNVQQARFIVDLEKARE"
     gene            2310..3665
                     /locus_tag="PLD82_05945"
     CDS             2310..3665
                     /locus_tag="PLD82_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64962.1"
                     /translation="MENHENQPETRATTAGNPHPDTPSGKGGHPAGTGKRPGQPGTQR
                     RFLAIIGAALVSFAAILSLLFFVVLPPSSSQKERTFNTELALSTALTFSSTAKHSPTE
                     AFSRFATSGATEHFRIIGERVWQTVSPELGWRWLFHGALMALSPPQPGGLTNQTIIGW
                     YHPWSDTTLLTLWTISDEAARIADCEFVMGDILRKRNLDKPSTTRAWARRRQYFPAAI
                     GITTAESLRSIELLTFHSNLPAEARSNIAALTNPPGSAGPASSRFWRHRLPYVLSASN
                     LEANHTGTGVMLLASISDITRFSAPTNVFAIRVVPTLQQLHSPSNLLTIGPEAAATIG
                     NLPKDFFSRFVPALYANNRQRAFLMLQSKTDPKLCLAFLFKLEPPDGKELTPEDEKKL
                     DEARTRQITALTNLDQVGTNETPEQMYPEAHYPIKLIRADLINLDAVYTNRYPETKGK
                     K"
     gene            3662..>3854
                     /locus_tag="PLD82_05950"
     CDS             3662..>3854
                     /locus_tag="PLD82_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64963.1"
                     /translation="MNRFRWFLLVCIAGSLTGAEMVYQMVTTLPPLFSGVEANSTATL
                     QMLIDEQTTAVTNRADANRI"
BASE COUNT          852 a         1285 c         1015 g          702 t
ORIGIN      
        1 gctcgcgtgc cttttcgaga tcgacgatga accgggcctg ctggacattc cggtatgctt
       61 tgatggaaag ctccttggcc agcttgaaaa ggaaatccgc aatcttccgg aaacgctcct
      121 ccgtcatgat cggaacccgc ttcatcgcct cccggtaccg ctcgggatcg gccccgatct
      181 cccgggcgta ctccatcatc cggccctcat ccagatcacc gcaacggacc tgcccgacca
      241 gccagttggc gatatgccgg ccaccgaccg taatgctcgc gcccgcatcc caaagccccc
      301 cagacaggca aggttggaca ttcggcccgt cgggattgta ccgcccaacc agggcatcag
      361 agagcctgca gcgctccctg ccaatttccg tcgaccggat gacttcctgg cagagttcac
      421 aaaaattgcc tggccgggtg accgggatcc cgtcaggcgt cgtgatcagc gaagcaacgc
      481 cggtggcttc agaaaaagca tcctggatgg cctgcagttc gtccatgtcg aagagtgacg
      541 caagatctac cccccccgat ccgccatcac ccgtcagcat ggtgatgcgc atccgcaacg
      601 cttcctcggc ccgcgaccgc tccgtgatat catcaaacat gatgatcacc cggccgcccg
      661 caagctgggt caggacgaca acctcgcgca caactccgtc ccgacagcgc acggtcagat
      721 ccgtggaatg accctgcttc ccgctctgtg ccgatccgga cttggcatgt tcccaaagaa
      781 ggcgcaacgc ctcctgcccg gaggcatcgg caatcaccag tcccgcccag gatgcgattg
      841 tcggtgtttc cgccagatcg tatccgaaga aggtgatgaa tcgccggttg agaaattcga
      901 accggccgtc cggataggcc caggcgatcg gtaaaggaga actgtcgaag agagcctgac
      961 gccgcgcttc cccttcgcgc aaccggcgat tgatcctgac gatcgggagc acaatcccca
     1021 gaataagaag aagggccgtg agccctgcgg caaaaaccca ttgcaggcgg gaagaaccat
     1081 ccccttcatc gctggtccag ataaagccgt caagcgagta atttttcggg aggagtccca
     1141 gttcggcgta ggtgtcggcg atatgtctcc agcgcccggg gttgatgtgc ccgatatcga
     1201 ccagttcggg caggacgagt ttgcgcatct ccttcatctc gaattcgagg aaggcccggg
     1261 agatccccct gtcataacgg ctcttgatga gatcgatgat ctgctgcggt ctctcgaagg
     1321 catagcgcca acccttgagg ctggcctcac ggaaccgtcg gaccatctcc gggttgccat
     1381 ccacgatctt tccgtccgca aagaggcagt cgttgtaaaa atccacccct ccctgctcgg
     1441 gtaaaaaccg gcgggtctcg actccggcat gcctgagaaa aaacggttcc gtcgtaacat
     1501 acgccgtcat ggcatcagcc ctgcccgtga cgatatcgag cgggttgtgg gagtgcgtga
     1561 ttttgaaaaa gttgttgacg ccttcattcc gtaaaaaagc ttcaatctcg gcggcctgtg
     1621 gttcgaacat gatccgcttc ccggtcaact catggaccgt cttgatcggg gaagactccg
     1681 gcacaagaac cgcgtacggt gaatgctgac agattgccgc caggacaaca acatccttcc
     1741 ctttgtacct gttgatgatc aattcggagg atccgatacc gaactgcgcc cgaccggagg
     1801 cgacctcgtc caccggatcg agcccgggcg ttgcaggccg gatctcgaca tccaggccgg
     1861 catcggcgta ataccccaac tccctggccg tatagtaccc ggcgaactgg aactggtgcc
     1921 gccacttgag ctggagcaca accttgtcga cggcctgcaa aaaattcggg aagaagccca
     1981 cacacagaag aatggccagc ataccggccc ggttcagtcg ctgcatatcg gctccgcaat
     2041 ccctttttga ccaagtatag cacatacggc aagccaatgc gacacagtgc cgggaaagac
     2101 ccgcatgaaa cagccttccc cgttcccgga cacatccttc ccgggcatca gggcgttcat
     2161 caaaaaaaag agaccggacc gcttgccgcg gaccaatcct gacaggaagc cgtcccaacg
     2221 cgacccggat tgggcgattg cggacacgaa cggccaatcc ggacacaagg ggccttgcct
     2281 ttccatgcgg cttcagatag aatcgtgaca tggaaaacca cgagaatcag cccgaaacca
     2341 gggccaccac tgccggaaat ccccatcctg acacgccgtc aggaaagggc gggcaccctg
     2401 ccgggactgg caagcgcccc ggacaacctg gcacacagcg ccggttcctc gcgattatcg
     2461 gcgcagcctt ggtgtcgttc gcagccatcc tgtccctgct tttctttgtg gttctcccgc
     2521 catcctcgtc ccagaaggaa cggacgttca acaccgaact cgctctctcc acggcactca
     2581 ccttctcctc aaccgcgaaa cacagcccga ccgaggcctt ctcccggttt gccaccagcg
     2641 gagcgacaga gcatttcagg atcatcggag aacgggtctg gcagaccgtc tcccccgaac
     2701 tgggctggcg ctggcttttt catggcgccc tgatggccct cagcccgccc cagccggggg
     2761 gattgaccaa ccagacgatt atcgggtggt atcatccctg gagcgatacc acactcctca
     2821 cgctctggac aatcagcgat gaagccgccc ggatcgcgga ttgcgaattc gtcatgggtg
     2881 acatcctccg gaaacggaat ctggacaagc cgtccaccac ccgggcatgg gccagacgac
     2941 gccagtactt cccggccgcc atcgggatca cgacagccga atccctgcgg agcatcgaac
     3001 ttctcacatt ccactccaat ctcccggcag aagcccgcag caatatcgct gccctgacca
     3061 accctcccgg gagcgccgga ccggccagtt cccgattctg gcgtcaccgt ttgccctatg
     3121 tcctctcggc ctccaacctc gaagccaacc acacggggac cggagtcatg ctcctggcct
     3181 cgatcagcga tattacccgc ttttccgctc cgaccaacgt cttcgccatc cgagtcgtcc
     3241 cgaccctcca gcaattgcac tccccgtcca atcttctgac gatcggaccg gaggcagccg
     3301 ctacgatcgg gaatctcccg aaagactttt tcagccgctt tgtcccggcc ctctacgcca
     3361 acaaccgcca gcgggccttc ctcatgctgc aatcaaagac ggatccaaaa ctctgcctgg
     3421 ccttcctctt caaactggag ccacccgacg gaaaggaact gacccccgag gacgagaaga
     3481 agctggatga agcccgcacc agacagatca cggcactgac caatcttgac caagttggca
     3541 caaacgagac cccggagcag atgtaccccg aggcccacta tccgatcaaa ctgatccggg
     3601 ccgatctgat caatctggat gcggtctata ccaaccgcta cccggagacg aagggcaaaa
     3661 aatgaacaga ttccgctggt tcctgcttgt gtgtatcgcc ggctccctga caggtgccga
     3721 gatggtctat cagatggtca caacgctccc gccactcttc tccggtgtcg aagcgaacag
     3781 taccgcaacc ctgcagatgc tgattgatga acagacgacc gccgtgacaa accgggcgga
     3841 cgccaacagg atcg
//
LOCUS       DAOSHE010000182         3853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21704_length_3853_cov_4.963665, whole genome shotgun sequence.
ACCESSION   DAOSHE010000182 DAOSHE010000000
VERSION     DAOSHE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21704_length_3853_cov_4.963665"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLD82_05955"
     CDS             <1..253
                     /locus_tag="PLD82_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64964.1"
                     /translation="ARLIEAAIPGVRKSRQDAMPWVIGTILLMKERLYHEALVYVSRA
                     ISVNDLAPFPFTLRGRIYEEMNLPNLALQEYQVASRLGQ"
     gene            complement(350..988)
                     /gene="pgsA"
                     /locus_tag="PLD82_05960"
     CDS             complement(350..988)
                     /gene="pgsA"
                     /locus_tag="PLD82_05960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOG64965.1"
                     /translation="MIWNIPNILTITRILLVPVFLVFILGPDFPSRLASFFVFVTAAI
                     TDFLDGRIARLFNQRSEFGKFADPLADKLLVAAALLAFTGIPETGIPLWMVLLLLGRE
                     IGITLLRLWAIHRKTSMETSRFGKCKTASQMATIIVVLLLLILRSALAESLGLSLSQE
                     DFWKIALNDAFWGTVLTWLPLALASITTTLTIFSGLAYLWVNRALFARRRSS"
     gene            complement(985..2349)
                     /locus_tag="PLD82_05965"
     CDS             complement(985..2349)
                     /locus_tag="PLD82_05965"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOG64966.1"
                     /translation="MQRFFLATLGCAKNVVDSADARRALLAAGWVETDDASSADLLVV
                     NTCGFITPAKQESIDTILDLAQHKKDGARLVMAGCFVQRNAQELAASLPEVDGFFGIS
                     FADALASHPDHSGVFPLPVPVGTEPYSPRLGASFDAGPGSAWLKISDGCDNRCSYCAI
                     PDIRGPYRARPAADILSEARILAESGIQEINIISQDTSRYTDPDQPGMDLIRLLDEIE
                     RLDGPRWIRLLYLHPATTSPALIQRMLSGGKVLPYFDLPVQSLVDSTLARMKRRTTWA
                     DVSRLVETIRSKNSQATIRSTLITGYPGETPKDHALTIERLQQLSFDKLGAFVWSPEE
                     GTPAASERPRVTEATANRRLDEIMRTQQEISSRLLARYIGKTLPILVTGSTLDPESIP
                     PEGLPLDPSAPSCWGRGPADAPDVDGLACATIPAGKPVPKPGSFAEIRIESSTEYDLY
                     GALA"
     gene            complement(2358..3404)
                     /locus_tag="PLD82_05970"
     CDS             complement(2358..3404)
                     /locus_tag="PLD82_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG64967.1"
                     /translation="MDTVGQILKAAREKRGDTINEIAIHTKIGSRYLSALEEDRYEAF
                     PSETHITGFIRSYAKYLELDPDHLIGIYKRILLQEAPTPIEELTAPRKQHVNPAFLIA
                     VFVGILIIAIPLIFILGHKKSGETVLDDDSQSSRQQDTPSPERSFSQGDFIPVMIAGE
                     EKRLVFDALTPETVTVSYAGQRYPLAVGETHSLDFNKDGNFDVRIQIKNVVDQKMHGS
                     VTLGKQPVPEETNETNKPAEHGTSGAGKAILKADEQVEIRLTITANGMATVNTLRDNN
                     ERSDFFLKKGDKITILAKDTVQLTASNPNNLLLNLNNVNLSVDTKSPAAGFLFKWRRN
                     PADGLFYLEYEQLR"
     gene            complement(3410..>3853)
                     /locus_tag="PLD82_05975"
     CDS             complement(3410..>3853)
                     /locus_tag="PLD82_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOG64968.1"
                     /translation="SNGKLLWVYLPYFGIVAEQDLVKNENEYTALLGRSKKSLDQLKQ
                     DYSFRFADGGRDNPESYILSLEPRVSKIGFKSIRLWVNKESGLVTRVEAWTVNSKPLT
                     ITFSNIKTDTELQPTIFWFGMPDANVQVIRNTILPVDIFDKARRQ"
BASE COUNT          792 a          921 c         1311 g          829 t
ORIGIN      
        1 ggcccgtctg atcgaggcgg cgattcccgg ggtgcgcaag agccggcagg atgcgatgcc
       61 ctgggtgatt ggaacgattc tcctgatgaa ggaacgcctt tatcacgagg ctctcgtgta
      121 cgtatcgcgg gccatcagtg tcaacgatct ggcgccattt cccttcacgc tcagaggccg
      181 gatctacgaa gagatgaatc tccccaatct ggctttgcag gagtatcagg tcgcatccag
      241 actcggccag taaacctccg gatgcaggat ctgtccgagc tgacagcagc acagttgatg
      301 gccgattgtt ccccaaaaag atgttggaag atgtgctgaa agaccttttt tatgatgaac
      361 gacgtctggc gaagagcgcg cgattgaccc agagataggc aaggcccgag aagatggtga
      421 gagtcgtggt gatggatgcc agggctaagg gaagccatgt gaggacagtt ccccagaaag
      481 catcattgag ggcgatcttc cagaagtcct cctgggagag tgacagtcca agggactcgg
      541 cgagggctga acgaaggatg agaagaagaa ggacgacgat gatggtggcc atctgggaag
      601 cggttttgca tttgccgaaa cgcgaggttt ccatgctggt cttgcggtga atggcccaca
      661 gtcgcagaag ggtgatgccg atctcgcgac cgagcaggag gaggaccatc cagagcggga
      721 tgccggtttc gggtatgccg gtaaaggcca ggagagcggc ggcaaccaag agcttgtcgg
      781 ccaaggggtc ggcgaatttg ccgaattcgc tgcgttgatt gaagagtcgg gcgatccggc
      841 catcaagaaa atccgtgatg gcggccgtga caaagacaaa aaagcttgcg agccgggacg
      901 ggaaatccgg accgaggatg aagacgagga agacagggac gagcaggatg cgggtgatcg
      961 tgaggatatt gggaatgttc cagatcatgc cagcgctccg tagagatcgt actcggttga
     1021 agactctatg cgaatttcgg caaaactgcc cggtttcggg accggtttcc cggcggggat
     1081 cgtggcgcag gcaagaccat ccacgtccgg agcgtcggcc ggaccgcggc cccagcagga
     1141 cggagcggac ggatcgagag ggagtccctc gggggggatg gattccggat caagggttga
     1201 tcctgtgacc aggatgggca gggtttttcc gatgtatcgg gccagaagcc gggaagagat
     1261 ctcttgctgg gtgcgcatga tttcatcaag gcgacggttg gccgttgctt cggtgacgcg
     1321 ggggcgttca ctggctgccg gggttccttc ttcgggggac cagacaaagg cgccgagttt
     1381 gtcgaaggag agttgttgca ggcgttcaat ggtcagggcg tggtccttgg gtgtttcccc
     1441 cgggtagccc gtgatcaggg ttgagcggat cgtggcctgc gaatttttgg atcggattgt
     1501 ctccacgagc cggctgacat cggcccaggt tgtgcgacgt ttcatcctgg caagcgtcga
     1561 atcgacgagg gactgaacag gaaggtcaaa gtaggggagg acctttccgc cggaaagcat
     1621 gcgctgaatc aatgccggcg aggttgtggc cggatggagg tagaggagac ggatccagcg
     1681 ggggccgtcc aggcgttcga tctcgtccag caggcggatc aaatccatcc cgggttggtc
     1741 ggggtctgtg taacgggagg tatcctgcga gatgatgttg atctcctgga tacccgattc
     1801 ggcaagaatc cgggcctcgg aaaggatgtc cgcagcaggc cgggctcggt aggggccccg
     1861 gatgtcggga atggcgcaat agctgcaccg attgtcacac ccgtcggaga tcttgagcca
     1921 ggcggacccg gggccggcat cgaaggatgc gccgagtcgc ggggaatatg gctcggtacc
     1981 aaccggaacc gggagaggaa aaacgcctga atgatcagga tgcgaggcca gggcatcagc
     2041 gaaagagatc ccgaaaaatc cgtcaacctc cggcagggat gcggccagct cttgggcatt
     2101 gcgctggacg aaacagcctg ccatgacaag ccgtgcgcca tcttttttgt gctgggccag
     2161 gtcgaggatg gtgtcgatcg actcctgttt ggctggcgtg ataaagccac aggtgttgac
     2221 gacgagcaga tcggccgagg acgcgtcgtc cgtctcgacc cagcccgctg ccagaagggc
     2281 gcgccgggca tcggctgagt cgacgacatt ttttgcgcag cccagggtgg cgagaaaaaa
     2341 acgctgcacg gggcggatta ccgcaactgc tcgtattcga ggtaaaagag cccgtcggcc
     2401 ggattgcggc gccacttgaa gaggaatccg gcagcggggc tttttgtgtc aaccgagagg
     2461 ttgacgttgt tgaggttgag gagcaagttg ttgggattgc tggccgtcaa ctggaccgtg
     2521 tctttggcaa ggatcgtgat tttatccccc tttttgagaa agaaatcact gcgctcgttg
     2581 ttgtcacgga gtgtattgac ggtagccatg ccgttggcgg tgatggtcag gcggatctcg
     2641 acctgttcgt ccgctttgag gatggctttg ccggcaccag acgtgccgtg ttcggcaggt
     2701 ttgttggttt cgttggtttc ttcggggacg ggctgtttcc cgagagtgac cgaaccatgc
     2761 atcttctggt cgacgacgtt cttgatctgg atgcgaacat cgaagttgcc gtctttgttg
     2821 aaatcaagag agtgcgtttc gccgactgcg agaggatagc gctgtccggc gtagctgacg
     2881 gtgaccgttt cgggtgtgag agcgtcaaag acaaggcgtt tctcctcgcc ggcgatcatg
     2941 acagggatga aatcaccctg ggaaaaagac cgttccgggc tgggtgtgtc ctgttggcgt
     3001 gagctctggc tgtcatcatc aagcactgtt tctccgctct tcttgtggcc gaggatgaaa
     3061 atcaggggta tggcgatgat gagaatcccg acaaagacgg caatgaggaa ggccggattg
     3121 acgtgctgct tgcggggggc tgtcagctcc tcgatcgggg tcggagcttc ctggaggagg
     3181 atgcgtttgt agatcccgat caggtgatcg ggatcgagtt cgaggtattt ggcgtagctg
     3241 cggatgaagc cagtgatatg ggtttcactg gggaatgctt cgtagcggtc ttcttccagc
     3301 gccgagaggt agcgcgaccc gattttggta tggatcgcaa tttcattgat agtgtcgccg
     3361 cgcttttcac gcgcggcttt gagaatctgt ccgactgtat ccatgctgct cactgtctcc
     3421 ttgccttgtc aaaaatatca acggggagga tggtgttgcg gatgacctgg acgttggcgt
     3481 cgggcatgcc gaaccagaag atggttggct gcaactcggt gtccgtcttg atattgctga
     3541 aggtaatggt cagcggcttg ctgttgacgg tccaggcttc gacacgggtg acgagaccgc
     3601 tctccttgtt gacccagagg cggatcgatt tgaaaccgat tttgctcaca cggggttcga
     3661 gggagaggat gtagctctcc gggttgtcgc gtccaccatc cgcaaaacgg aagctgtagt
     3721 cctgcttgag ctggtcgagg ctctttttcg aacgcccgag gagtgcggtg tattcgtttt
     3781 cgtttttgac gagatcctgc tcggcaacga tgccgaagta cggaagatag acccagagga
     3841 gttttccgtt gga
//
LOCUS       DAOSHE010000183         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_21867_length_3834_cov_2.882244, whole genome shotgun sequence.
ACCESSION   DAOSHE010000183 DAOSHE010000000
VERSION     DAOSHE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21867_length_3834_cov_2.882244"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3510)
                     /locus_tag="PLD82_05980"
     CDS             complement(<1..3510)
                     /locus_tag="PLD82_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64969.1"
                     /translation="MPLEAGILPDSGYDFLLPADLDVLTAEATRILSREERLIEILKR
                     RLSNPGLSLDAPPRLFSFLGPDSLLAELDEGMRIRVLRGRILDRLEDPFGDLGSGYRD
                     DLFQGWRLARERARDADQKLIDTRIGGRDGGWRWDISSDVRLVLRGQQEITALWGFDS
                     VDQNLQEGKYVKRVEVARDFRLAQNLDINLVGSIGPRISVNINHNTMRRENEYDIRYT
                     GAKEEILKEIAAGTIDVSLPGSKYVVFSGGSKAAFGVKAALENDFFKMQAVLSLTKGV
                     SETKEFRGQRSEQKVLLRDTSWVKHRYFRIPDTGLDKNSFRLYLTTTLTNGSLLIAGG
                     LYTVLVEGKDYVLNQSSGQLDLAKPVTRDQNLLAVRTVGGSDTPGLVHPAPPWAGHER
                     VQDLERSFVYLKRVGYYSRFERKGIYLTGYRNIELSRGFELAVVTTADGVRSSEVQFP
                     TDAWRLNPETGMLEFQALEPFDGKPGVPEILYHSQNDPESSDSVYSISMAFLHRVDSF
                     QLRYNVMPGSERVYLNGAIQTRDKEYRIDYTTGELVFLVALGESDTIRVSYEYKPYGN
                     ALQRTLFGTRADIRPVDGLEFGTSLFYSGGQKPTGAPQPVATPDSQMILAQDARIDLM
                     KLFGIKRPEWRVRIEAEAAYSVHDRNRVDRAIFADMEADVTGYAITRSEEKWWPGSPS
                     RAVNGGLAQAGRGRLLYRNYRDYGLSGDYSLESYNWNLPSSQIYGYARKPGPYTAGGG
                     RNESTSKISENSLVMEYDLSGGRTWVNAVSSIAGAGGLDLSRISQLLLSLRHQGVRGF
                     DGNGNPLYGDVAGSPVTIHFEIGRLDEDSDGDGVFDREETVGSGGWAFTPAGAESTWP
                     GGGRRNAGNGVIDGEDLDGDGVLSTVEESVLFPAAGFTEPAVASIPQGGGWQELTFRI
                     ENLSQSNLAILSRANAVRITITGAGGERGRIMIDRAWFKGSTWDVLRIDDTVVPDRTL
                     PAFSASVISTRENSEYRDNRIFREYRDDFEDLHGTMSSTEESEFNEKALQIQWSLSNM
                     PRDSGGTGSFGSVTRVYNTTLDFSFYRKMVLYMFVKEKAGDTNEAFVFRLGNSDDRYY
                     QWRIPLTSLEKAGDAVSAGRKKWHKLEFDLDPGTTGERFVVRKDGVVLARATTSAKKP
                     NLREVTRATVGVDTTGSLLPDNRG"
     gene            complement(3644..>3834)
                     /locus_tag="PLD82_05985"
     CDS             complement(3644..>3834)
                     /locus_tag="PLD82_05985"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOG64970.1"
                     /translation="VKQMITVMRKSIEKSGFIDGGNAEEIFTDMLYDQYADSMSRNGD
                     FGLSKTIYAQLTGGKKWV"
BASE COUNT          815 a         1206 c         1017 g          796 t
ORIGIN      
        1 ccccctgttg tccggcaaga gactccccgt tgtatcgacc ccgacggtgg cccgtgtcac
       61 ttcgcgcaaa ttgggcttct tggcactggt tgtcgcccgt gccagaacca ccccgtcttt
      121 tcggacaaca aagcgctccc cggtagtccc cggatcgagg tcaaattcca gcttgtgcca
      181 ttttttgcga cccgcactga cggcatcccc ggctttttcc agcgaggtga gcggaatacg
      241 ccattggtaa tagcggtcat cgctgtttcc caggcggaaa acaaaggctt cattggtatc
      301 cccggccttt tctttgacaa acatgtagag gaccattttc cggtagaacg aaaaatcaag
      361 agtcgtatta tagacccggg tcaccgaacc aaaactgccc gtcccgccgg aatcacgggg
      421 catattggaa agactccact ggatctggag agccttttcg ttgaattcac tctcctccgt
      481 actggacatc gtcccgtgca ggtcttcaaa atcatcgcga tactcacgga agattctgtt
      541 gtcacggtac tcgctgtttt cccttgtcga aatcacgctc gccgaaaagg ccggaagtgt
      601 ccggtccggt acaaccgtat cgtcgattct cagcacatcc catgtggacc ccttgaacca
      661 ggcccgatcg atcatgatcc tgcctcgctc gccaccggca ccggtgatcg tgatcctgac
      721 cgcattggcc cgtgaaagta tcgccagatt ggattgcgag aggttttcga tcctgaaggt
      781 caattcctgc cagccgccac cctggggaat gctggccact gcaggttccg taaagccggc
      841 cgccggaaag agcaccgact cttcgacagt cgagagcacc ccgtcaccat ccaggtcctc
      901 gccgtcgata acgccatttc cggcattccg cctcccgccg ccgggccagg ttgactcagc
      961 cccggccgga gtgaaggccc agccgccact cccgacggtt tcctcgcggt caaaaactcc
     1021 atcgccatcg gaatcctcat ccagtcgtcc tatctcgaag tggatggtga ccggcgagcc
     1081 tgccacatcc ccatagaggg gattcccgtt cccgtcaaat ccccgcacgc cctgatgccg
     1141 cagggagagc agaagctgac tgatccggct caagtcaagg cctccggcac cggctatcga
     1201 ggaaacggca ttgacccagg tccgcccacc ggagaggtcg tactccatga ccagggaatt
     1261 ctcgctgatc ttcgaggtgc tctcgttccg gccaccacca gccgtatacg ggccgggctt
     1321 gcgggcatac ccatagatct gtgatgacgg aagattccag ttatagctct cgagggagta
     1381 gtcacccgaa aggccatagt cccgataatt ccggtagagc aaccggcccc gcccggcctg
     1441 tgccaacccg ccattgaccg cccgggaggg ggagccgggc caccacttct cctcggagcg
     1501 ggtgatggcg tagcccgtca catcggcctc catatctgcg aagatggccc ggtcaaccct
     1561 attccggtca tggacggaat aggccgcctc tgcctcgatc cggacccgcc actccggtcg
     1621 cttgataccg aacagtttca tcagatcgat ccgcgcatcc tgtgccagaa tcatctgcga
     1681 gtcgggagta gccaccggct gtggcgctcc ggtgggcttc tgccctcccg aatagaacaa
     1741 ggatgtcccg aattcgaggc cgtccacagg ccggatatcg gccctggtcc cgaagagtgt
     1801 ccgctggagg gcattgccgt agggtttgta ctcgtaactg acccggatcg tatcactctc
     1861 gcccagggca acgagaaaga cgagttcgcc cgtcgtatag tcgatacgat attccttgtc
     1921 ccgtgtctgg atcgctccgt tgagatagac ccgctcactc cccggcatga cattgtagcg
     1981 caactggaag gaatccaccc gatgcaagaa tgccatcgag atggagtaga cagaatcgct
     2041 cgactcggga tcgttttgcg agtgatagag gatctcggga actccgggct tcccgtcaaa
     2101 gggctcaagc gcctgaaact cgagcatccc ggtttccgga ttgagccgcc aggcatccgt
     2161 cggaaattga acctcactgc tccgaactcc gtcggccgtt gtgaccaccg ccagttcgaa
     2221 tcccctcgag agttcaatat tcctgtaccc ggtcagatag atgccctttc gctcgaaccg
     2281 ggagtaatag ccaacgcgct tgaggtagac aaaactccgt tcgagatcct gcacacgttc
     2341 gtgcccagcc caggggggag ccggatggac cagccccgga gtgtcgctcc cgccaactgt
     2401 ccggaccgca agaagattct ggtcgcgggt aaccggcttg gcgagatcaa gctgcccgct
     2461 tgactgattg agtacataat ccttgccctc gacaagaacc gtatacagac cgcctgcaat
     2521 caggaggctg ccgttggtga gggttgttgt caggtagagc cggaaggaat ttttgtccaa
     2581 ccccgtatcc ggaatccgga aatagcggtg cttgacccag gatgtatccc ggagaaggac
     2641 cttctgctca ctgcgctggc ccctgaactc cttggtctca ctgacaccct tggtcaggga
     2701 gaggacggcc tgcatcttga aaaaatcgtt ttccagagca gctttgaccc cgaaggcggc
     2761 cttggaacca ccggaaaaca caacgtattt gcttcccgga agggagacat cgatcgttcc
     2821 ggccgcaatt tccttgagga tttcttcctt ggcacctgtg tagcggatgt cgtactcgtt
     2881 ttcacgacgc atggtattgt gattgatatt gacactgatg cgcggtccga tggaacccac
     2941 caggttgatg tcaagattct gtgccagcct gaaatcccgg gcaacctcga cacgtttgac
     3001 gtatttcccc tcctggaggt tctggtcgac actgtcaaac ccccagaggg ccgtaatctc
     3061 ctgctggccc cggagcacca gccggacatc actgctgata tcccaacgcc aaccgccatc
     3121 acggccgcca atcctggtgt cgatgagttt ttggtcagca tccctggccc gttcgcgggc
     3181 gagccgccaa ccctgaaaca ggtcatcacg atagccgctt ccgagatccc cgaacgggtc
     3241 ctcgagccgg tccagaatcc tcccgcgcag gacacggatc cgcatgcctt catccagctc
     3301 cgccagcagg gaatccggac caagaaacga aaacaaccgt ggcggagcat cgaggctcaa
     3361 acccgggttg gagagtctcc gtttgaggat ctcaatgagc ctctcctcac gtgacaggat
     3421 ccgggttgcc tcggctgtca atacatccag atcggctgga agcaaaaagt cgtaccctga
     3481 atcgggcagg atccctgctt cgagcggcac gaaacaaagg cacagcaaaa gaaccgcagc
     3541 caaaaagccg cgtccggaaa tgccaggtct ttccgggtac acgaacatgc cgtcagaaat
     3601 cctccctgct gcccgcgtga accggtgtcc ctcgctgacg aatttacacc cacttcttcc
     3661 cgccggtaag ctgggcatag atcgttttcg agaggccgaa atcaccgttg cggctcatgc
     3721 tgtccgcata ttgatcatac agcatatcgg taaagatctc ctccgcattc cctccatcga
     3781 taaaaccgct tttctcgatg gacttgcgca tcacggtgat catctgtttg acca
//
LOCUS       DAOSHE010000184         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22131_length_3804_cov_4.291278, whole genome shotgun sequence.
ACCESSION   DAOSHE010000184 DAOSHE010000000
VERSION     DAOSHE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22131_length_3804_cov_4.291278"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLD82_05990"
     CDS             complement(<1..439)
                     /locus_tag="PLD82_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG64971.1"
                     /translation="MNDDRALHTTAGDDPGDILSAVFSTSPDGIAIASLEGTVIAASP
                     RALEMWGYTDSSEVVGRNMLEFIDERDRERAIFLIGGLLQGRPTGAAEYLMLRKDGNR
                     FYCESNANVIKNNAGQPIAIMIVERDMTERHNAAEALRQSEEKY"
     gene            470..1171
                     /locus_tag="PLD82_05995"
     CDS             470..1171
                     /locus_tag="PLD82_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64972.1"
                     /translation="MSRRRPVRRAERFHEKLVLYGNVSIKGDEMRSMLCLVVCVASLP
                     MLSFAQAQPPLPVISPDIQLAADFFAEEALGMWYGRLGPVTASANLPAASGISYGPAN
                     LHDFSPLTAWAVPDGGVGQSVTWRFAQAWSDEVYAFNGRIVIVNGYAKSEALFKENNR
                     VRSLVVYYNGRLFCRLALEDSRELQVFDLSTLRSRLPARKVNDTFCFRIESIYKGTKY
                     NDTCISEMLFGSNGN"
     gene            1174..2415
                     /locus_tag="PLD82_06000"
     CDS             1174..2415
                     /locus_tag="PLD82_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG64973.1"
                     /translation="MKTARIRFFFQHIRAGWMAGCVFVLMVSSACASSPAAQAARAGS
                     TSQGSADDWFKKAVELDGQGRVAEALIWYQRAAEGKPGNGFYPARLGQHLLTKMLDWP
                     NAVRAYQRAIGLGFTQSWMWSQYAHALRWLGETAFSRGDWKDARGIFDEAVRVSQTAG
                     VRTNVIPYLQHMSVLSRQAPFAATRTAEVRHRSLMILLEDTGRSGEPRRRIKAKEAEL
                     ARMSFDFMRHWVRTATSGLVFIEGEVAVFREPVNALVPLEDGSLQLDAALASRHLGSI
                     LDSRAGEVDSIILIWASDKHQTAHGGATRYSLDAGRRSVWRGHVQIPSLRMAHNGPYL
                     LCHEFFHVVENMCGIRPRHGFEAANRGSFPGWTGSDEFSYYGWSFSDNIPRILASPEW
                     RALGWKHFRFTHKYPVKNERQ"
     gene            complement(2356..3096)
                     /locus_tag="PLD82_06005"
     CDS             complement(2356..3096)
                     /locus_tag="PLD82_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64974.1"
                     /translation="MEHANLAWLTLALCASISLLVCGARAAASARLRRAFCFFHGSFG
                     LLLATHLVNRYLALNLPGSDPNLLAFVRYLENPALLLVLAVAAPVLVHELTDCPQRAL
                     RNLLSAGLALAMLFLNYSISLFHTRGDATRILIKDGLFILIVAYCMLLLVRAWQRERD
                     TNVRRRRRRTALIMSALFPAIIADTFFDASLEFKFFPIVHLVFGIDLFGYLDVPAPAQ
                     SGRPTPPARLLALVFDRIFVRKTEVLPA"
     gene            complement(3083..3247)
                     /locus_tag="PLD82_06010"
     CDS             complement(3083..3247)
                     /locus_tag="PLD82_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64975.1"
                     /translation="MPKQHNTHEDAAKKAIDVLLKRDWKDIHASEHGKRPGVAPVRSA
                     HKALHTHGTR"
     gene            complement(3289..>3804)
                     /locus_tag="PLD82_06015"
     CDS             complement(3289..>3804)
                     /locus_tag="PLD82_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64976.1"
                     /translation="SVRPVCQFSTPYRPYTLFAGNGLAFLAEYSEDSEGGAKPEGHII
                     TLSSGQRRAIPVDYVIYGAAFSPDGSRLYAASSMNGTLSEYDTASGVMRSRIPIGTHG
                     HVLGYAKPDTLFWIRNDGIVSIATTPAPRMTDYLGFTSIQQGFWHTDGSFITGNTAWV
                     KNGDELWKMNF"
BASE COUNT          721 a         1050 c         1226 g          807 t
ORIGIN      
        1 ggtacttttc ctcgctctgg cggagtgctt cagcggcatt gtggcgttcg gtcatgtcgc
       61 gttcgacgat catgatggca atgggttgac ccgcattgtt tttgatgaca ttggcgttgg
      121 attcgcagta aaaccggttg ccgtccttgc ggagcatgag gtattcggca gcacctgtcg
      181 gtcggccctg gaggagtcct ccgatcagaa agatggcacg ttcccggtcc ctttcgtcga
      241 tgaactccag catgttccgg ccgacgactt cgctgctgtc agtatacccc cacatttcga
      301 gggcccgggg cgatgctgcg atcacggtcc cttccaggga ggcgatcgcg ataccgtcag
      361 gagaggtcga aaagacagcg gagaggatgt ctcccggatc atctccggcc gtggtgtgca
      421 gtgcccgatc gtcattcatg tgcaccacta tgcatgcgga ggcgttgttt tgtcaaggcg
      481 tcggcctgtc cgccgtgcgg aacgatttca tgaaaaactt gtcctctatg gcaacgtttc
      541 catcaagggg gatgagatgc gatccatgct ctgcctcgtt gtctgtgttg ccagcctgcc
      601 gatgctttcc tttgcacagg cgcagccgcc gctgcctgtg atcagtcccg atatccagct
      661 cgcggccgat ttttttgctg aagaggccct cggtatgtgg tatggccggc ttgggcctgt
      721 gacggcctcg gcgaacctcc cggcggcctc cgggatcagc tacggaccgg ccaacctcca
      781 tgatttttcg cccctgacgg cctgggctgt tccggatggc ggtgtcggcc agagtgtcac
      841 ctggcgcttt gcccaggctt ggtcggatga ggtgtacgcc ttcaatggac ggattgtcat
      901 cgtgaacggc tatgcgaaaa gcgaagccct gttcaaggaa aacaaccgcg ttcgctccct
      961 tgtcgtgtat tacaatggcc gcttgttctg tcgactggcc ctggaggata gccgggagtt
     1021 gcaggttttc gatctctcca cgttgcgcag ccggctgccg gcccggaaag tcaatgacac
     1081 gttctgtttc cgtatcgagt ccatctacaa ggggacaaag tacaacgaca cctgcatcag
     1141 cgagatgctt ttcggcagca acgggaactg acgatgaaga cagcaagaat ccgttttttc
     1201 tttcagcata tacgtgccgg ctggatggct ggctgcgtct ttgtcttgat ggtctccagt
     1261 gcctgtgcct ccagtccggc tgcgcaggca gcccgtgccg ggtcaacgtc acagggctcg
     1321 gcggacgact ggttcaaaaa agccgtcgaa ctggatggac agggccgtgt tgccgaagcc
     1381 ctgatctggt atcagcgggc agccgaaggg aagccgggaa acgggtttta cccggcccgt
     1441 ctggggcagc atttgctgac aaagatgctg gactggccaa acgccgtccg ggcgtatcag
     1501 cgggcgatcg gcctcggctt cacacagtcc tggatgtggt cgcagtatgc ccacgccctg
     1561 cgctggcttg gggagaccgc gttttcacgg ggggattgga aggatgcccg cggtattttt
     1621 gacgaagccg tccgggtctc acaaaccgcc ggcgtgcgga ccaatgtgat tccctatctc
     1681 caacacatgt ccgttctcag ccgtcaggct ccgttcgcgg ctacacgcac agccgaggtc
     1741 cgccaccggt cactcatgat actgcttgaa gacaccgggc ggagcggaga accccggagg
     1801 cggatcaagg ccaaagaggc cgaactggcc cggatgtcct ttgatttcat gcgccactgg
     1861 gtgcggactg ccacgagcgg gttggtcttt atcgaaggag aagtcgcggt ttttcgggaa
     1921 ccggtgaacg cccttgtgcc gctcgaggat ggttctctcc agctcgatgc ggctttggcc
     1981 agccggcacc tgggttccat tcttgacagt cgcgccggag aggtggacag catcatcctg
     2041 atctgggctt ccgacaagca tcagaccgcc catggcgggg caacgaggta ttctctcgat
     2101 gctggccggc gctcggtctg gcgggggcac gtgcagattc cctcgctgcg gatggcgcac
     2161 aacgggccct acctcctctg tcacgaattt tttcacgttg tcgaaaacat gtgcgggatc
     2221 aggccgcgcc acgggtttga ggcggccaat cgcggcagct tccccgggtg gacgggcagt
     2281 gacgaatttt cgtactacgg ctggagtttc tcggacaaca ttccgcggat tctcgcatcc
     2341 cccgagtggc gcgctttagg ctggaagcac ttccgtttta cgcacaaata tccggtcaaa
     2401 aacgagcgcc aatagtcggg cagggggcgt gggtctgccg ctctgcgcgg gagccggcac
     2461 gtcaaggtat ccgaacaggt cgattccgaa gacgaggtgg acaatgggaa agaacttgaa
     2521 ttcgagcgag gcatcgaaaa atgtgtccgc aatgatggct ggaaaaaggg cactcatgat
     2581 cagggctgta cgtcgtcggc gccggcgcac gtttgtgtcc cgctcgcgct gccaggcacg
     2641 gacaagcagg agcatgcagt aggccacgat caggatgaag agtccgtcct tgatcaggat
     2701 gcgggtcgcg tctccccggg tgtgaaagag gctgatactg taattgagaa agagcatggc
     2761 aagggccagt cccgcggaca gcaggttgcg gagggccctt tgcgggcagt ccgtcagctc
     2821 gtggaccagc acaggggccg cgacggcaag aaccagcagg agggccggat tttcgagata
     2881 gcggacaaag gccagcaggt tgggatcgct cccgggaaga ttgagggcca gatagcggtt
     2941 gacaagatgg gtagccagca gcaatccgaa gctgccatgg aaaaagcaaa acgcccggcg
     3001 caggcgtgcg gaggcggcag ccctggctcc acagaccagc aggctgatgc tggcgcagag
     3061 ggccagtgtc agccaggcga gattagcgtg ttccatgggt gtgcagggcc ttatgggcag
     3121 accggacggg ggcgacgccc ggtctcttgc cgtgctcact ggcgtggatg tctttccagt
     3181 cgcgcttcaa taaaacgtcg atggcttttt ttgcagcatc ttcgtgggta ttgtgttgct
     3241 tcggcattgt cactcccagg gttagtgcca ctacgtccgg aatgtctgtc agaaattcat
     3301 cttccagagt tcgtcgccgt ttttgaccca ggcggtattt ccggtgatga aggagccgtc
     3361 ggtgtgccag aacccctgct ggatggatgt gaatcccagg tagtcggtca tgcgtggtgc
     3421 cggggtcgtg gcgatcgaga cgattccgtc attgcggatc cagaagaggg tgtccggctt
     3481 ggcgtatccc agaacatgcc catgggtgcc gatcgggatc cgtgagcgca tgacgccgct
     3541 cgccgtgtca tattcgctca gggtcccgtt catggaggaa gcggcgtaga gtcgggatcc
     3601 gtcgggtgaa aaagcggcac catagatgac gtagtcgacg ggaatcgcac gcctttgccc
     3661 gctggaaagt gtgatgatgt gtccctccgg cttggcgcca ccctcgctgt cttcggagta
     3721 ttcagcaaga aaagccaggc cattcccggc aaacagggtg tacggacggt atggagtgga
     3781 gaactggcag accggacgga cgct
//
LOCUS       DAOSHE010000185         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22173_length_3799_cov_4.013355, whole genome shotgun sequence.
ACCESSION   DAOSHE010000185 DAOSHE010000000
VERSION     DAOSHE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_3799_cov_4.013355"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLD82_06020"
     CDS             complement(<1..345)
                     /locus_tag="PLD82_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG64977.1"
                     /translation="MATTMIHATIDGRPVEVEAGTTIMQAAETIGIRIPRLCYHPKLS
                     LEGACRICIVDVKGMRFFPASCATKVTEGMEITTGSPEIRQARRDIVELLLDNHPMDC
                     QTCERDGNCELQN"
     gene            complement(345..2468)
                     /gene="nuoF"
                     /locus_tag="PLD82_06025"
     CDS             complement(345..2468)
                     /gene="nuoF"
                     /locus_tag="PLD82_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG64978.1"
                     /translation="MHQRMKAHQIEIHIGDATCENAAGSLDVRDEFEKLIEASGRSDI
                     VIKKTGCTGRCSAEPIVGVMQHGKPPFKYQSVTPEKVRRIFTEHILNGQIVLDYLLDK
                     KTKNEYRYQVAFYKNSTFCGAEPFDAMEVAKAGLHKAGLDETAVKLSLCLNRGLFISH
                     CDVQYYMIVFPQIVRYRIRSQADLDRVLTGHLVGGQIVTELVEEEDALFDRYLTTYAD
                     IEFFSKQTRLTLRNSGIVDPESLDDYVFYCGFGAAARVLDGMTPEEVVAEVKKSGLRG
                     RGGAGFLTGLKWEFAQKAKNPKRYIICNADEGDPGAFMDRSTLEGDPFSIIEGMIIGA
                     YATGANQGFCYIRAEYPLAIQRVELAIEKAREAGLLGKNILGSGLDFDLEIRLGAGAF
                     VCGEETALMHSIEGRRGQPRIRPPFPAESGLWGCPTTINNVETWANIPVVFQYGADWF
                     ARIGTEKSKGTKVFALAGKVKNTGLVEVPMGTTLRNVIFDIGGGIPGDKTIKAVQTGG
                     PSGGCIPEEMLDTEVDYDSLAAIGSIMGSGGMIVLDEDSCMVDVAKFFLEFTMSESCG
                     KCTPCREGTLRMYEILERITRGESREDDLDRLERLANLVKKTALCGLGQTAPNPVLST
                     LKHFRDEYEIHVKEKRCPAKVCKKLIVYEIDKDKCIGCTACARVCPVNCIAGKPKTPH
                     EIDKNKCIRCGQCWTTCKFDAIRKI"
     gene            2760..>3799
                     /locus_tag="PLD82_06030"
     CDS             2760..>3799
                     /locus_tag="PLD82_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64979.1"
                     /translation="MTRSARISLWSACLVASTLLLSCGTDFLFEELEPPRDITIPAPT
                     GLTATDGTLMARIRLTWLATPEAVTYQVFRSNLPEGGAFTMVASNLPSATWDDTNTVA
                     NYPFYYKVAFLTATSNQSELSTTARGMSLPWQAPAPLDVQARAGSSEDKVRISFSAGS
                     NTTAVSWVIYTAPGEDAPYSPIATIPATNTTSMETGSLDRGMTHFFRVVGLDAGGTAS
                     QVSRAASWAIPPALALPAPTALTASSGTDNTGITLAWTGAPGADGYRIYRTSATNTGY
                     QLVAGVPTSPWKDTNAIAPDRFLYRISATNSSEESLADQPVEGWRGYGPWNGATILSA
                     SDGSTSSGILLT"
BASE COUNT          773 a         1246 c          959 g          821 t
ORIGIN      
        1 attttgcaac tcgcaattcc cgtcgcgttc gcaggtctgg caatccatcg gatggttgtc
       61 cagcaggagc tcgacgatat cccgtctggc ctgacggatc tcgggcgacc cggtcgtaat
      121 ctccatcccc tcggtcacct tggtcgcaca ggatgccgga aaaaaccgca tccctttgac
      181 atcaacaatg cagatcctgc aggcgccctc gagggagagt ttggggtgat agcagagtct
      241 cggtatccgg atgccgatcg tctcggcagc ctgcatgatc gtcgttcccg cttcgacctc
      301 cacgggtcga ccgtcgatcg ttgcatgaat catagttgta gccatcagat cttccttatc
      361 gcgtcgaatt tgcacgtcgt ccagcactgc ccgcaccgga tgcatttgtt tttgtctatc
      421 tcgtgcggag tcttgggttt cccggcaatg cagttgaccg ggcagacacg ggcacaggcc
      481 gtacacccga tacacttgtc cttgtcgatc tcgtacacga tcaatttttt gcacaccttg
      541 gccgggcaac gcttttcctt gacatggatc tcgtattcat cccgaaagtg cttgagcgtc
      601 gagaggaccg ggtttggtgc cgtctgcccc agaccgcaga gtgccgtctt tttgaccaga
      661 tttgccaagc gctcaagccg atcaaggtca tcctcgcggg attccccgcg cgtgatccgc
      721 tcgaggatct cgtacatcct gagcgttccc tcgcgacagg gcgtacattt tccgcaggac
      781 tcgctcatcg tgaactcgag gaagaacttg gcaacatcca ccatacagct gtcctcgtcc
      841 agcacgatca ttccgccaga acccatgatc gaaccgatgg ccgccagact gtcataatcc
      901 acctcggtat ccagcatttc ctcggggata caaccacccg agggccctcc ggtctggacc
      961 gccttgatcg tcttgtcccc gggaatcccg cctccgatgt cgaagatcac gttccgcagg
     1021 gtcgttccca tgggcacttc aaccagtccg gtgttcttga ctttcccggc cagtgcaaag
     1081 accttggttc ccttgctctt ttctgtcccg atccgggcaa accagtccgc cccgtactgg
     1141 aacacgaccg ggatattggc ccacgtctcg acattgttga tcgtcgtcgg acacccccat
     1201 aacccgcttt cggccggaaa cggggggcga atcctcggct ggccacgccg tccctcgatt
     1261 gaatgcatca gcgctgtttc ctcaccgcag acaaaggcgc cggccccaag ccggatctcg
     1321 agatcaaaat ctaacccgga acccaggatg tttttcccga gcaatccggc ctcgcgtgct
     1381 ttttcgatcg ccaattccac ccgttggatc gccaatggat actctgcacg gatatagcaa
     1441 aagccctggt tggccccggt tgcgtaggcc ccgatgatca tgccttcaat aatggaaaaa
     1501 ggatcccctt ccagtgtgct ccgatccatg aacgcacccg ggtccccctc gtccgcattg
     1561 cagatgatgt accgcttggg atttttggcc ttttgcgcaa attcccattt caggccggtg
     1621 agaaaaccgg caccaccacg acctcgcagt ccggacttct tgacttcggc cacgacctct
     1681 tccggtgtca tgccatccag caccctggcc gctgcaccaa acccgcaata gaagacatag
     1741 tcatccaggc tctccggatc gacaatcccc gaattcctca gggtcaacct cgtctgcttg
     1801 ctgaaaaact cgatatcggc gtatgttgtc agatagcggt caaagagggc atcttcctcc
     1861 tcgacaagct cagtgacaat ctgccctcca accaaatgac cggtcaagac gcggtcgaga
     1921 tcggcctgac tccggatccg gtagcggacg atctggggaa agacgatcat gtaatactgc
     1981 acgtcgcagt gtgagataaa gagtccccga ttgagacaga gcgagagctt gaccgctgtc
     2041 tcgtccaagc cggccttgtg caacccggct ttggcgacct ccatggcatc aaacggctcg
     2101 gcaccgcaga acgtgctgtt tttatagaac gcgacctggt agcgatactc gttttttgtt
     2161 tttttgtcga gcaggtagtc cagcacgatc tgcccgttga ggatgtgctc ggtaaaaatc
     2221 cggcgcacct tttccggtgt caccgactga tacttgaacg ggggcttccc gtgctgcatc
     2281 acgccgacaa tcggttcggc actgcaccgc ccggtacacc cggtcttttt tatgacaatg
     2341 tcgctccgcc cggacgcttc gatgagcttt tcaaattcgt cccgtacatc cagggagcca
     2401 gccgcattct cgcatgtcgc atcaccgata tggatctcga tctggtgcgc cttcatacgt
     2461 tgatgcatgc gctcctttgt ctccgcctgc agcttggcat actgctcaag gattgtaccc
     2521 acagtccgca cccctttacg ttcgttgccg taccgttgat acggcgcact tcgccaacca
     2581 gtatcccgta tttgacaaat gcttgtcaag ccaaaacacg ctttgaatct ccagagacgg
     2641 aagacttggc agatctgttc gcaacagatc cgggacaata atccggcaac ccggcgtcga
     2701 caggctcagt attatgcagt atcatcatat tgtgcatacc atgccccccg gaggtcgaaa
     2761 tgacacgatc cgctcgcatc agcctgtggt cagcctgcct tgtcgcttcg acactcctcc
     2821 tctcctgcgg gacggatttc ctttttgagg agcttgaacc tcccagggat atcaccatac
     2881 cggcaccgac aggactgacc gcgaccgacg ggaccctgat ggcccgcatt cgtctgacgt
     2941 ggcttgcgac tcctgaagca gtgacatacc aggtcttcag gagcaatctc cccgagggcg
     3001 gcgcctttac catggtcgct tcgaatctcc cctcggcgac ctgggacgac accaacacgg
     3061 tcgccaatta tccgttttac tacaaagtgg cctttttgac ggcaaccagc aaccagagcg
     3121 agctttcaac cactgccaga ggcatgtccc tgccctggca agccccggcc cctctcgacg
     3181 tccaagcccg agccgggtcg tccgaagaca aggtgcgcat cagtttttcc gcaggcagca
     3241 atacaaccgc cgtatcctgg gtgatctaca cggcccccgg cgaggacgcc ccctactcac
     3301 ccatcgcaac cattcccgcc accaacacaa ccagcatgga aacagggtct cttgatcgag
     3361 gtatgaccca tttcttccgt gtcgtcgggc tggatgccgg aggcacagcc agccaggtca
     3421 gccgcgccgc atcctgggcc atccccccgg ccctcgccct tcccgcacca accgccctga
     3481 cagcttcgtc cggaaccgac aacacgggga tcaccctggc ctggacggga gccccaggtg
     3541 cggacgggta ccggatctac cgtacatccg caaccaacac cggctatcag cttgtggccg
     3601 gagtcccgac atcgccatgg aaagacacca acgcaatcgc ccccgatcgc ttcctctaca
     3661 ggatcagtgc aaccaacagc agcgaggaga gcctcgccga ccaacccgtc gagggttggc
     3721 gcggctatgg cccatggaac ggcgccacca tcctcagtgc ctccgacggg agcacaagca
     3781 gcgggattct gctcacctg
//
LOCUS       DAOSHE010000186         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22265_length_3788_cov_3.271631, whole genome shotgun sequence.
ACCESSION   DAOSHE010000186 DAOSHE010000000
VERSION     DAOSHE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22265_length_3788_cov_3.271631"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3443)
                     /locus_tag="PLD82_06035"
     CDS             complement(<1..3443)
                     /locus_tag="PLD82_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOG64980.1"
                     /translation="MRLVRILVGVFLCIPLAGFTDVHQTPASQYLWNGYGPRVSALGF
                     TGISMLGDPDAGGANPAVLGDLRRLANGLSVTGLGADSPLWSGSLAVPTDIGVLSAHL
                     LYGTSSQTNAALDTAGLALRIAKPITEDLFWGFGLSFLYADYTTGLDRGLWADMGILW
                     RIPHKGRGIGLFDPSVGLVLRGMGIRLSNEGDDRYPPLALGLGFSVTAVDIGLWSLRL
                     QADSMLAIDPLNPVLNFGFENTVFNFLKIRLGYMTGDTRHGMSGLGSMTFGIGLAGKL
                     RLSEKADPTDFDLSWALVNQHFHGKAERMHSFGLTVAWGTYDSQAPDARVKPDWARFS
                     PNYDGRQDEVRFGLSISDDTLVTGWVLDILDQTGKTNRSFRSIDRFELRSLNFEKFVK
                     AIFSSKQQVDIPRHIDWNGQDEAGKMVADGVYAWKLTSWDENRNVSEKRGTVRVDTRI
                     PQVRAISTNSIFSPNGDGARETYSFGNILIAAESDDVLEVKILAPDGTPVRAWTYSGA
                     FPDVHTWDGKTSAGSEAPEGVYSISFEAYDPAGNRTEDRIAGVRLVRRYETVRIEAVK
                     EAFSPTGDGSNDLLRIRSSVSDSNGLQHWTLVVRDSEQRAVRSIRGTGAVPPELVWDG
                     KSDSGLRQPDGFYTAELGLEYDSGNHPVSRRIPLEVDTTPAKVEIRPEYLEFSPNGDG
                     NRDGLPVALKGSGQTQDAIRLQVLDENGIAVLSRDWTLASLPPVWNWDGNDAGGKPVH
                     EGWYTIRIETRDRVGNTGTSLVENVRLKTGLEKVSLAGSRSAISPGAAGKNGQVIFTP
                     AVSSKDSIERFDVVIKDSDGRTVRTLTNARHVPEIPWDGRDEQGNTLKDGRYTYLLKV
                     KYSYGDAPVSVERTVLIDTRPPVIRVKAGDSIFSPNADSRKDTITIEQEVQGGDDDVY
                     HGEIVDDTGRVLRSHKYVGSVPAQFVWDGKDGNGRDLPEGRYTWRLRGMDAADNTASS
                     TTTPVTLVRRMGSIGLTARPMAFSPNGDGQEDQLGLAIAPSTKEGIEKAELVIWDLAR
                     RAVYRQRWTGSLPEIHTWNGLGSDGKPQLDGWYEAEVTVDWESGNQARAVVEKILLDA
                     RPPEIGLTVQPRIFTPDGDGEDDVLHINPRMQDVSGVAEWEIVISKHESDGKTAKPFK
                     T"
     gene            3563..>3788
                     /locus_tag="PLD82_06040"
     CDS             3563..>3788
                     /locus_tag="PLD82_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG64981.1"
                     /translation="MKSVLIDQKTCTICQRCIRICPTSVLRSIDGKILAKYPERCIAC
                     GQCFAFCPTGSISLNGCDPREALPCAAGTLP"
BASE COUNT          770 a         1201 c         1022 g          795 t
ORIGIN      
        1 aatgtcttga acggtttcgc agtcttgccg tccgattcgt gcttcgaaat cacgatctcc
       61 cattcggcaa caccggagac atcctgcatc ctgggattga tgtgcagaac atcatcctcg
      121 ccgtcaccat ccggtgtaaa gatcctcggt tgaacggtca aaccgatttc cggcggcctg
      181 gcatcgagga ggatcttttc gacaaccgcc cgggcctgat tcccgctttc ccagtcgacc
      241 gtcacctcgg cctcgtacca tccgtcaagt tgcggcttgc catcactccc caacccgttc
      301 caggtatgga tctcgggcag actgcccgtc cagcgttggc ggtagacggc gcgccttgca
      361 agatcccaga tgaccagttc ggccttctcg attccttcct tggttgaagg ggcaatcgcc
      421 aatccgagct ggtcttcctg cccgtccccg ttgggagaaa aggccatcgg tctggctgtc
      481 aagccgatac tgcccattct ccgcacaagg gtgaccggtg tcgtcgtcga actggcggtg
      541 ttgtctgcgg catccatacc ccgcagacgc catgtatacc gcccctcggg aagatcccgg
      601 ccgttcccgt ctttcccgtc ccagacaaac tgggccggta cactgccgac atacttgtgg
      661 ctgcggagca cccttccggt atcatcgaca atctcgccat gatacacgtc atcatcccca
      721 ccctgtacct cttgctcgat ggtgatcgta tccttccggg aatccgcatt cggcgagaag
      781 atgctgtcgc ccgccttgac ccgaatcaca ggaggccgtg tatcgatcag gacggtccgc
      841 tcgaccgata ccggggcatc cccgtaactg tacttgacct tgaggagata ggtatagcgt
      901 ccgtccttga gggtatttcc ttgttcatca cggccgtccc agggaatctc gggaacatgc
      961 cgggcattgg tcagggtacg gactgtccgg ccatcgctgt ccttgatgac aacatcaaaa
     1021 cgttcgatgc tgtccttcga ggaaaccgcc ggagtaaata tgacttgtcc gttttttcct
     1081 gccgctcccg gggatatggc actccggctc cctgccaggg agactttttc cagtccggtc
     1141 ttcaagcgga cattctcgac cagacttgtt ccggtattcc cgacccgatc ccgggtctcg
     1201 atccggatcg tataccagcc ctcgtgaacc ggcttcccgc ccgcatcatt cccgtcccag
     1261 ttccataccg gtggcagact tgcgagcgtc cagtcccggg acaagacggc aattccgttc
     1321 tcgtcgagca cctgcagcct gatggcatcc tgtgtctggc cgctcccctt gagggcgacc
     1381 ggaagaccgt cacgattccc gtcaccattg ggcgagaatt ccagatactc cggccggatc
     1441 tcgaccttgg cgggggtcgt atccacctca agcgggatcc ttctcgaaac cgggtgattg
     1501 ccgctgtcat actcaagccc gagttccgcc gtataaaacc cgtcgggctg tctgagcccg
     1561 gaatcagact tcccgtccca gacgagttcc ggtggcacgg cacccgttcc ccggatgctc
     1621 cgcacggcgc gttgttcact gtcacgaacc accagagtcc agtgttgcag gccatttgaa
     1681 tccgaaaccg aagaccggat ccgcaaaaga tcattggagc catccccggt cggtgaaaag
     1741 gcttccttta cggcctcgat ccggacggtc tcataacggc gcaccagccg gacaccggca
     1801 atcctgtcct cagtccggtt gccggcggga tcataggcct caaagctgat ggagtatacg
     1861 ccttccggtg cttccgagcc ggcggatgtc ttgccgtccc atgtgtgcac atccggaaac
     1921 gctccgctgt acgtccaggc acggacgggt gtcccatccg gagcgagaat cttcacttcc
     1981 aggacgtcat cgctctcggc cgcgatcagg atgttcccaa aactgtaggt ctccctggca
     2041 ccatcgccat tgggagaaaa aattgaattt gtcgagatcg cacggacctg ggggatcctg
     2101 gtatccaccc ggacagtacc ccgtttctcg ctgacattcc tgttctcgtc ccaggatgtc
     2161 aacttccagg catacacacc gtctgccacc attttcccgg cttcatcctg tccgttccag
     2221 tcgatatgcc ggggtatatc cacctgttgc tttgatgaaa atatcgcttt gacgaatttt
     2281 tcgaaattca gactgcgcag ctcgaagcgg tcgatactgc gaaaactgcg attggtcttc
     2341 cctgtctggt caagaatatc cagaacccat cctgtcacaa gggtatcgtc gctgatcgag
     2401 agcccgaaac ggacctcgtc ctgccgtccg tcgtaattcg gtgaaaaacg ggcccagtcc
     2461 ggtttgaccc tggcatccgg agcctgcgaa tcataggtcc cccaggccac tgtcaggcca
     2521 aagctgtgca tccgttcggc cttgccgtgg aaatgctggt tgacgagggc ccaggagaga
     2581 tcaaaatctg taggatcggc cttctcgctc aaacggagtt tcccggccag accgatcccg
     2641 aaggtcatcg agcccaggcc actcatcccg tggcgggtat cgccggtcat gtatcccaga
     2701 cggatcttca ggaagttgaa gaccgtattt tcgaatccga agttgagtac cgggttgagc
     2761 ggatcgatcg ccagcatgct gtcagcctgg agtcgcagac tccagagtcc gatgtcgacc
     2821 gccgtcaccg aaaagcccag gccaagggcc aatggcggat agcggtcatc tccctcgttc
     2881 gagaggcgta ttcccatccc ccggaggaca agtccgacag acggatcaaa aagaccgatt
     2941 ccgcggccct tgtgcggaat ccgccagagg atgcccatat cggcccagag tccgcgatca
     3001 aggccggtgg tgtaatcggc atagaggaag gagagcccga acccccagaa aagatcctca
     3061 gtgatcggct tggcgatccg cagggcgagc ccggccgtat cgagggccgc attggtctgc
     3121 gaggatgtcc cgtacaaaag gtgcgccgag agaacaccga tgtccgtggg aacagcgagc
     3181 gaacccgacc agagcgggga atccgcgccc agtccggtca ccgagagccc gttggccaac
     3241 cgcctcagat cacccaagac cgccggattg gcgcctcccg catcggggtc cccaagcatg
     3301 ctgatgccgg tgaacccgag ggccgagacg cggggaccat acccgttcca gagatactgg
     3361 ctggccggag tctgatggac atcggtaaac cccgccagcg gtatgcagag aaaaacacca
     3421 accaggatac gtaccagtct cataatactc ccctcctcgt gcaatgccgg gaacaaccgc
     3481 catacttctc aacgtcaaat atacgaatat cctcaccggg ctacatcctg agtatgcttg
     3541 atggatatcc ggggaggttg gaatgaaatc tgtgctcatc gaccaaaaga catgcaccat
     3601 ctgtcaacgc tgcatccgga tctgtccgac ctccgttctg cgaagcatcg acggaaaaat
     3661 tcttgcgaaa tatccggagc gctgcatcgc ctgtggccag tgctttgcct tttgtccgac
     3721 gggcagcatc tcgctgaatg gctgtgatcc acgagaggct cttccctgcg ctgccgggac
     3781 cctgccgg
//
LOCUS       DAOSHE010000187         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22454_length_3768_cov_4.395906, whole genome shotgun sequence.
ACCESSION   DAOSHE010000187 DAOSHE010000000
VERSION     DAOSHE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22454_length_3768_cov_4.395906"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLD82_06045"
     CDS             <1..369
                     /locus_tag="PLD82_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64982.1"
                     /translation="PVHYLIVPRVLAGVIVLPLLTLFTDLVAIFGGMLVAFLEMGLNE
                     TQYIETTLRMLTISDINNGLIKSVVFGLLITLIASFEGFRTKGGAEGVGKSTTRSVVM
                     ICMSILFFDYVLTSLLTTSF"
     gene            380..1207
                     /locus_tag="PLD82_06050"
     CDS             380..1207
                     /locus_tag="PLD82_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG64983.1"
                     /translation="MTPEHNNAIAVRRLKKSLGGKEVLRGVDLEIRRGETMVIIGQSG
                     CGKSVLLKHIVGLHFPDQGEVFIDGQPLNGVSTSERLKLLSEFGMLFQGAALFDSLTI
                     AENVAFRHIQSRKYSQKEMDRIVAEKLEMVGLSGIAHLKPAELSGGMQKRVGLARAIA
                     ADPKFILYDEPTTGLDPIMADQINDLILHMQEVLNVTSIAVTHDMVSAYKIADRISML
                     YQGQVIETDTPDRIQRSKNPYVRQFIRGEAYGPITTVRPFGYTGPSGTKGGKHEAPQ"
     gene            1191..2072
                     /locus_tag="PLD82_06055"
     CDS             1191..2072
                     /locus_tag="PLD82_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOG64984.1"
                     /translation="MKRPNEFKTGILVLGGFAIFIFANIVIRGISFSQEGFTVFVRFP
                     TISGINAGSDVRLADGIKIGKVVAIEMKDGQAIVETRIKTGTKLTTGSRGSITSSTFL
                     SELFVSISSGTGLGRTLKGGETIEGEAPMSMVDGIREFGLLMKNLNSMISSGTDGSMV
                     RDIAAIVRNMASRIEQMLIASGTDVSQSFRNLNEATRKLNLLADEFTGSGTKLQTLAN
                     TVQGDLPAILANLKQSTASLVRLSEMAVSGQGSVAALFSSRKFYDDLNLILENLKVLT
                     VKLKNDPSIILWRNDNR"
     gene            2075..3526
                     /gene="radA"
                     /locus_tag="PLD82_06060"
     CDS             2075..3526
                     /gene="radA"
                     /locus_tag="PLD82_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG64985.1"
                     /translation="MAKSSSVFLCAACGFESSRWLGRCPECGEWNSFSEEHREPPAKH
                     AKQPARGPIQPVALSSILPVDHPRITTGSRELDQVLGGGLVPGAAVLAGGEPGIGKST
                     LLLQTAGYLAGQDQLVLYVSAEESSSQLAMRARRLGLGSVVSGSGTANGRNEQLTQMG
                     KILILESGSLPSIESAISTLRPTLVIIDSIQAVHSEGLSSTPGSVSQVRECAWFLSEE
                     CRRLGSALILAGHVTKEGALAGPKTLEHMVDTVLYFEGQRDRELRLIRCVKNRFGNVN
                     EIAVFMMTDHGLSEVTDPSGIFIDGITPKGPGSIMTATLEGNRVLLAEIQALVTDRVA
                     GNLARTTDGVDTNRVMRIRAILENRIAIDLADSDIFVNVTGGLVIDEPSAELAIAAAI
                     AGSLSNRIPDCPVVVMGEIGLLGDLRRIPHADKRVAQAAKLGVRAVVLPEANRRDLEA
                     VRSRGMASGQPQTGPDLVFAPTLATALDAVFGK"
BASE COUNT          794 a         1132 c         1073 g          769 t
ORIGIN      
        1 ccggtccact acctgatcgt cccccgcgtc ctcgccggtg tgatcgtcct tcccctcctg
       61 accctgttta ccgatctggt cgccatcttt ggaggcatgc tggtggcctt cctcgaaatg
      121 ggtctcaacg aaacgcagta catcgagaca accctccgca tgctcaccat cagcgacatc
      181 aacaacgggt tgatcaagtc cgtggtcttt ggccttctga tcacactgat cgccagcttt
      241 gaaggcttcc gcaccaaggg tggtgccgaa ggtgtcggaa aatcgacgac ccgttcggtc
      301 gtgatgatct gcatgtccat cctctttttt gactacgtcc tgacctctct tttgacgacc
      361 agtttctaag gagaatcgca tgacaccgga acataacaat gccatcgcgg tccgccgtct
      421 caaaaaatcc ctcggcggaa aagaggtcct gcgcggagtc gatctggaga tcaggcgcgg
      481 ggaaacgatg gtgatcatcg gacaatccgg ttgcggaaaa tcggtccttc tcaagcatat
      541 tgtcggactg cattttcccg atcaaggaga agtctttatc gatggccaac cgttgaacgg
      601 cgtgagtacg agcgagcggc tgaaactcct gtccgaattc ggaatgctct tccagggcgc
      661 cgccctcttt gattccctga cgatcgcgga gaatgtcgcc ttcaggcata tccagtcgcg
      721 gaagtactcg caaaaagaga tggaccggat cgtggccgaa aaactcgaga tggtcggcct
      781 ctccggaata gcacacctca aaccggccga actctcggga gggatgcaaa aacgggtcgg
      841 tctggcacgg gccattgccg ctgacccgaa attcatcctc tatgatgaac cgaccacggg
      901 actggatccg atcatggcag accagatcaa tgacctgatc ctgcacatgc aggaggtcct
      961 taacgtgacc tcgattgccg tcacccatga catggtcagc gcgtacaaga tcgccgaccg
     1021 gatctccatg ctctaccagg gccaggtgat cgaaaccgac acaccggacc gcatccagcg
     1081 ttccaaaaat ccgtatgtcc gccagttcat ccgcggagag gcgtacggtc cgataacaac
     1141 cgtccggccc ttcggctata caggaccatc aggtacaaag ggagggaagc atgaagcgcc
     1201 ccaatgagtt caagaccggg attctggtcc tgggtggatt tgccatcttc atctttgcca
     1261 atatcgtcat caggggaatc agtttttccc aggaaggatt tacggtcttt gtccgttttc
     1321 cgaccatttc tggcatcaac gcgggaagcg atgtccgcct ggctgacgga atcaagatcg
     1381 gaaaagtggt ggccatcgag atgaaggacg ggcaggccat cgtcgagaca cggatcaaga
     1441 ccggaacaaa actgacaacg ggaagccggg gatcgatcac cagctccacc tttttgagtg
     1501 aactctttgt ctcgatcagt tccggtaccg ggctggggcg gaccctcaag ggaggggaaa
     1561 ccatcgaggg ggaggcaccg atgtccatgg tcgacgggat ccgcgaattc ggcctcttga
     1621 tgaaaaatct caactcgatg atctccagcg gaaccgacgg ttccatggtc cgtgacatcg
     1681 cggccatcgt gcgcaatatg gccagccgga tcgagcagat gttgattgcc agcggaaccg
     1741 atgtctcgca atccttccgc aacctgaatg aggcgacgcg caagctgaat cttttggcgg
     1801 atgaattcac ggggagcggg acaaaactgc aaaccctggc caacaccgtc cagggggatc
     1861 tcccagccat tctggccaat ctcaagcagt ccaccgcaag cctggtccgg ctttcggaga
     1921 tggcggtcag cggtcaggga agcgtcgcgg ccctcttctc aagccggaag ttttacgatg
     1981 atctcaacct gatcctggag aacctcaagg tcctgacggt caaactgaaa aacgatccct
     2041 cgatcattct ttggcgtaac gacaatcgct gaccatggcc aaatcatcgt ccgtctttct
     2101 ctgtgcggct tgcgggttcg agagttcccg ctggctcggg cgctgtcccg agtgcggcga
     2161 atggaacagc ttttcggagg aacaccgtga gccccctgcc aaacacgcca agcagccggc
     2221 ccgtggtccg atccagcctg tcgctctttc aagcatcctc cctgttgacc acccgcggat
     2281 cacgaccggc tcacgggaac tggaccaggt cctgggtggt ggtctcgtcc ccggggcggc
     2341 cgtcctggcc ggcggggaac cgggcatcgg taaatcgaca cttctcctgc agaccgccgg
     2401 gtacctcgcc ggccaggacc agcttgtcct gtacgtgagt gccgaggaat cctcttccca
     2461 attggccatg cgggccagac gcctcggtct gggctcggtg gtatccggaa gcgggaccgc
     2521 gaacggtcgt aacgaacaac tcacgcagat gggaaagatc ctgatcctcg agagcggaag
     2581 cctcccctcc atcgagagcg cgatcagcac actccgtccg accttggtca tcatcgactc
     2641 gatccaggcg gtccacagcg agggattgtc gtccaccccg ggctccgtca gccaggtccg
     2701 ggagtgcgcc tggtttctct ccgaagaatg ccggcgtctc ggatcggcac tgattttggc
     2761 gggccatgtc accaaggagg gcgcgctggc agggcccaag acactcgaac acatggtaga
     2821 taccgtgctg tatttcgagg gacagcgcga tcgggaactc cgcctgatcc gctgtgtcaa
     2881 aaaccggttc ggaaatgtta acgagatcgc tgtcttcatg atgaccgatc acggactctc
     2941 ggaggtgacc gacccctcgg ggatttttat cgacgggatc actcccaagg gccctggctc
     3001 gatcatgacc gcgaccctcg agggaaatcg tgtcctcctg gccgaaatcc aggcccttgt
     3061 gacggatcgt gtggccggca atcttgcgcg aacgacggat ggtgtcgata ccaaccgggt
     3121 gatgcggatc cgcgccatcc tcgagaaccg gatagccatc gatctcgcgg acagtgatat
     3181 cttcgtgaac gtgaccggcg gtcttgtcat cgacgagcct tcagccgaac tggcgatcgc
     3241 cgctgccatt gccgggagtc tctccaaccg cattccggat tgtccggtcg tcgtgatggg
     3301 cgagatcggc ctcttggggg atctccgtcg gattccacac gcggacaaac gggtggccca
     3361 ggccgccaaa ctcggggtgc gggccgtggt cctgcccgaa gccaaccggc gggatcttga
     3421 agccgtccgc tcacggggga tggcctccgg ccaaccccag acgggaccgg atctcgtgtt
     3481 tgcacccacc ctggccacag ccctcgacgc ggtcttcggg aaataacatt cgtgatattc
     3541 tgattttcta cagtcccgat gtgcagattc atacctgccc gtcatggaaa taactgttca
     3601 gaggagttct cttggcaggg ggggaaagtc tctccctcgc gaagaggtca tcctgacaga
     3661 cgaagagtgc gaacatcctt gttcgccgtc tgttcgtccg catcctgctc ccagacagac
     3721 gaagagtgcg aacatccttg ttcgccgtct gttcgtccgc atcctgct
//
LOCUS       DAOSHE010000188         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22477_length_3766_cov_5.493398, whole genome shotgun sequence.
ACCESSION   DAOSHE010000188 DAOSHE010000000
VERSION     DAOSHE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22477_length_3766_cov_5.493398"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..920
                     /locus_tag="PLD82_06065"
     CDS             81..920
                     /locus_tag="PLD82_06065"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOG64986.1"
                     /translation="MILPVWLGEDLAFPDPALSHHQGLVALGGDLTPERLRLAYSMGI
                     FPWFSEDDPVMWWSPDPRTIFIPQAFHLSGSMRKVLRRHDFIIRINSAFSDIVQLCAS
                     IPRREQDGTWITPAMQAAYCRLHEAGDAHSIEVWQASPVFIRGTSSGRIISDQSGYTL
                     AGGVYGVLTGSVFCGESMFSLVPNASKIALAALMYLCIRSGILAVDCQFTTSHLESLG
                     AFEIPRQTYLDLLETDDDAHSPEHRFFPSSGSLFASPDWAMDLIDMLGESNPNGQDET
                     FPI"
     gene            complement(1029..2669)
                     /locus_tag="PLD82_06070"
     CDS             complement(1029..2669)
                     /locus_tag="PLD82_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG64987.1"
                     /translation="MSRMSVSKKLVLGFSIVAFLGCLSSALSIIYLNILDRDIEILSK
                     EAMPNITDLGEMRREVLTMRMALREMRNPLYDRTELESFVREYQRSQQEYLVAKGRYE
                     SVPMGSEEKALWERVIKAETDLFALNRAYVDYAVKHAGEKDMHGELLKLARQSGQAKA
                     YDEFIAFFNSQVAHIVKENRNINEQALRDSTNGRIGALIAGGASLILSLLIGLSLAKA
                     ITGRVRNATGVLETSADYIRSSSEQLASASQNLAAGSSEQAASIEQISSSLEEISSMT
                     KQNADYAAQANKLTVDAISSIDAAHKAMQRSLTASQEIARASGETQKIIKTIDEIAFQ
                     TNLLSLNAAVEAARAGDAGAGFAVVADEVRSLSMRSAEASRRTAQLIEETITKVNEGV
                     ATFAETGKSIDEVVERSARVQHLVGEIAAASNEQSKGIEQINRGVAEMEKVVQQNAAN
                     SEETAASTEELNAQAEEMRASVQALAAYVFGGGEKQSVQAEKKQPARLAPAQKVDRPV
                     VPPAPAKLVPPVPTQHVRTREFTPEQIIPLDEKDMQDF"
     gene            complement(2901..3713)
                     /locus_tag="PLD82_06075"
     CDS             complement(2901..3713)
                     /locus_tag="PLD82_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG64988.1"
                     /translation="MIINHNMSAHFVQRQLAGAGARMGDSIEKLSSGHRINKAGDDAS
                     GLAVSEKLRSQIRGIAQATRNAQNGISFLQTAEGSMQEMHSILHRMRELSIQGANQIY
                     STEDRLQIQVEVSQLKQEIDRIVDVTSFNQLKILDGSLKDMRFHVGPDKDQSIAVSID
                     SMDTKTLKVDAVDITTAQAANNSLGSIDGAVNAVSKQRANVGAWQNRMEHVVNALSVA
                     EENLTAAESRIRDTDMAREIIEFSRNEILTHTGTAMLVQANIQSKTVLKLLG"
BASE COUNT          758 a         1147 c          982 g          879 t
ORIGIN      
        1 ttcggccgac tgacacacgg tggccacgtc cttgttcacg ccaatcccga gggactcagc
       61 ttcgagattc ccgagctcgt atgatcctgc ccgtctggct tggagaggat ctcgcttttc
      121 ccgaccccgc gctctcgcac caccaggggc tggtggccct gggaggagac ctgacgccgg
      181 aacgtctccg tctcgcctac agcatgggga tctttccctg gttcagcgag gatgatccgg
      241 tcatgtggtg gtctcccgat ccccgaacga tcttcatccc gcaggccttc catctttccg
      301 ggagcatgcg aaaagtcttg cggcgtcatg atttcatcat tcgtatcaat tccgcttttt
      361 ccgatattgt ccaactctgt gcatcaatcc cgcgcaggga acaagacggg acgtggatca
      421 ctcccgcaat gcaggcagcc tattgcagat tgcacgaagc gggcgatgcc cattcgatcg
      481 aggtctggca agcctctccg gtttttattc ggggaacatc ctctggccgg atcatcagcg
      541 accaatccgg atatacgctc gcaggcggcg tctacggcgt cctgacaggg tcggtcttct
      601 gcggggaatc catgttctcc cttgtgccaa acgcctcaaa aatcgccctg gccgcgttga
      661 tgtacctttg catccgctcc gggatcctgg cagttgattg ccagttcaca acatcacacc
      721 ttgaatccct gggcgctttc gagatccccc gccagacata cctcgatctc ctcgagaccg
      781 atgatgatgc acactctccc gaacacaggt tcttcccatc atccggatcc ctctttgcct
      841 ccccggattg ggccatggat ctcatcgaca tgctcggaga gagcaatcca aacggacagg
      901 atgagacatt ccccatttga ctctgcgtct tccgggacac gccttgagca gaaggacaaa
      961 aaacacgggg tacaaccgcc acccagtgtc atcatcctga atccgggccg cccaacccct
     1021 caatggaatc aaaaatcctg catatccttc tcgtcaagag gaataatctg ctcgggtgtg
     1081 aattccctcg tccggacatg ttgcgttgga acgggtggga caagctttgc gggcgcaggc
     1141 ggcacaaccg gtctgtcaac tttttgggcc ggagccaggc gggcgggctg cttcttttcc
     1201 gcctggacgg actgtttctc gcctccgcca aacacatagg ctgccagtgc ctgaacgctg
     1261 gcccgcatct cttcggcctg ggcattcagc tcttcggtcg aagctgctgt ctcttcggaa
     1321 ttggccgcat tttgctggac aaccttttcc atttcggcaa ccccgcggtt gatctgttcg
     1381 atccctttgg actgttcatt ggatgccgct gcaatttcgc caacaagatg ctggaccctg
     1441 gcagaccgct ccaccacttc gtcaatcgat tttccggttt cggcaaaggt ggcgacacct
     1501 tcgttcacct tggtgatggt ctcttcgatc agttgggcgg tccgccggga tgcctcggca
     1561 gaccgcatgg agagagaacg cacctcgtcc gcaacaacgg caaacccggc accggcatcc
     1621 ccggccctgg ctgcttcgac agcggcattc aatgaaagaa gattcgtctg gaaggcaatc
     1681 tcgtcaatcg tcttgatgat cttctgggtc tctccgctgg ctctggcgat ctcctggctg
     1741 gctgtcaagg agcgctgcat ggccttgtgc gccgcatcaa tcgaactgat cgcatcaaca
     1801 gtcagtttgt tggcctgtgc cgcatagtcg gcgttctgtt tggtcatcga agaaatctcc
     1861 tccagcgagg acgagatctg ctcgatggaa gcagcctgct ccgaggaacc ggccgccagg
     1921 ttttgactgg ctgacgccag ttgttccgaa ctcgaccgga tgtaatccgc agaggtttcg
     1981 aggacaccgg tcgcattccg cacccgtccg gtaatagctt tggccagaga caggccgatg
     2041 agcaacgaca ggatcagcga ggcgcccccg gcaatcagtg caccgatccg gccgttagtg
     2101 ctgtcccgga gagcctgctc gttgatgttg cggttctcct tgacgatatg tgcgacctgg
     2161 ctgttgaaga aagcgataaa ttcgtcatag gctttggcct gtccggactg ccgggcaagt
     2221 ttgagcagct ctccatgcat atccttctcg cccgcgtgct tgacggcgta gtcgacatac
     2281 gcgcggttca gtgcaaaaag atccgtctcc gccttgatca cccgttccca gagagccttt
     2341 tcctcgcttc ccatcgggac ggactcatag cggcccttgg cgacaaggta ctcctgctga
     2401 ctcctctggt attccctcac aaaagattcc agctccgtgc gatcatacaa gggattccgc
     2461 atctcgcgaa gtgccatgcg catggtcaac acttcgcgcc gcatctcgcc caagtcggta
     2521 atattgggca tggcttcttt cgacaaaatc tcgatatccc tgtccaggat attgagatag
     2581 atgatcgaga gagcactgga caggcagcca agaaaagcca ctattgaaaa accgaggaca
     2641 agttttttac tgactgacat tcgcgacata ttgcctcctg cgacgcatta ctatcccttg
     2701 tggaaaatat acccgggaca cccgcatggc cgcaagggat taatcagcca tccgtgccga
     2761 tgcatgccat tcccgactct ctccctgttg cgaacgaacc acaaaaaaaa ttacacccgc
     2821 ctccgccgcc atccgggtag aatggtgtgg caggagcggg tgcaaccggt ccgtggactc
     2881 tatcaaaaca acagaactgt ctagcccaga agcttgagga cggtcttgga ctggatattg
     2941 gcctggacca gcatcgcagt cccagtgtgc gtcaggatct cgtttctcga aaactcgatg
     3001 atctcccgag ccatatcagt gtccctgatc ctggattccg cagctgtcag gttttcctcg
     3061 gcaacggaca gggcattgac gacatgctcc atcctgttct gccaggcacc cacattggca
     3121 cgctgctttg aaacagcatt cactgcgcca tcaatggagc cgagactgtt gttggccgct
     3181 tgcgctgtcg tgatgtcgac agcatcgacc ttgagcgtct tggtgtccat cgaatcgatc
     3241 gaaacggcaa tggactgatc cttgtccggt ccgacgtgaa agcgcatgtc cttgagcgag
     3301 ccatcaagaa tcttcagctg gttgaacgac gtaacgtcga caatcctgtc gatttcctgc
     3361 ttcagttgcg atacttccac ctgaatctga agcctgtcct cggtgctgta gatctggttg
     3421 gccccctgga tggacaactc ccgcatccgg tgcagaatcg aatgcatctc ctgcatcgat
     3481 ccttccgccg tctgcaggaa cgaaatgccg ttttgggcat tgcgtgtcgc ctgggcgatc
     3541 cccctgatct gcgaacggag tttttcggat accgcaagtc ctgacgcgtc atccccggcc
     3601 ttgttgattc tgtgccccga cgaaagcttt tcgatggagt cgcccatacg ggctcccgca
     3661 ccggcaagct gccgttgcac aaaatgcgca gacatattat ggttgatgat catactattc
     3721 cctccctcct ggaaatagcg cccgacatcc ctgccggtcg gctttg
//
LOCUS       DAOSHE010000189         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22488_length_3765_cov_3.335040, whole genome shotgun sequence.
ACCESSION   DAOSHE010000189 DAOSHE010000000
VERSION     DAOSHE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22488_length_3765_cov_3.335040"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLD82_06080"
     CDS             complement(<1..294)
                     /locus_tag="PLD82_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG64989.1"
                     /translation="MPDILVVDDNEANRYYLESLLGGHGYTVASAADGQAALECARRE
                     RPRLVVSDILMPVMDGYALCREWRLDPGLNAVPFVFYTATYTDDKDAEFALKLG"
     gene            414..1340
                     /locus_tag="PLD82_06085"
     CDS             414..1340
                     /locus_tag="PLD82_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG64990.1"
                     /translation="MNTDPVIKAYAITKRYGTLTAVDGLSFQVPPGTCLGLLGPNGAG
                     KTTMMKIIYGKGRRSGGDLQVFGYDPGRHALDVKACTGVVPQEDSLDAELDVRSNMRL
                     YARFYGINRQVADKRIEELLSFMELGDKNTARIRELSGGMKRRLVIARALLGNPRLLI
                     LDEPTTGLDPQVRHLIWDRLRKLRSSGTTILLTTHYMDEAYQLCDSLIIMDHGKAVLE
                     GNPRELVEKGIERYALQVFDQETGPDLRTRATDRAIRFEEAGGMLYAYADRLGDLEDL
                     AGILTARDYVLRQTNLEDLFLRATGKGLNDAQ"
     gene            1330..2199
                     /locus_tag="PLD82_06090"
     CDS             1330..2199
                     /locus_tag="PLD82_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG64991.1"
                     /translation="MHSDGFHAQDTPGDTALPPHPAGPSHTATGRLAPSWRQRIFAVW
                     FRHMRVYTKNLIANGFPPFFEPLLFLVAMGVGFSGAFSGKPVEGLPFLDFLASGLILT
                     SAMYTSAFECSYGTFIRLNYDKTYDSMLAAPLLPRDILIGEILWAGTKGAFFSSAVLS
                     VISVFGMIGTAGSLLSPLVGFITGLVFASASLLVTSFVRYNIDHFNFYFTGFLSPLFF
                     FCGILFPVSQLPAPLSSIAEALPLTHLVRLERAVCFGNWGPEHVWALVYALVFVLAVG
                     WLAIRRLEKQLID"
     gene            2274..3092
                     /locus_tag="PLD82_06095"
     CDS             2274..3092
                     /locus_tag="PLD82_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG64992.1"
                     /translation="MKRSTAVLVPGLITILTLAVFGYNHTAMSVLSGGVSEWNLWRNR
                     DPGREIDLSDADLKGRNLRSANFEQAILEQANLSEADLSYAKMGFAKLRRANFSKAKL
                     YRAILNDSDLSGASLNDAILTLAQLNNANLELCNLTGALLSEASLENSDATDANMSGA
                     QLPKANLSASKMIGANFKGANLAGAVFSQADLRNANFAGANLKGANFSGANLQNANLA
                     GAQVDDANFSWAKLQGAQIDRKNEAILRARGALQLAQVKWLQADDGVLRSGNGQ"
     gene            3287..>3765
                     /locus_tag="PLD82_06100"
     CDS             3287..>3765
                     /locus_tag="PLD82_06100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG64993.1"
                     /translation="MPENEYLAVLRALADSTRLRMLRTLGSCPSSAEDLATLLGLAPS
                     TVSFHLKKLEQAGLVSGKREQYYRIFSRDEQALTPRLLDIVDREPARSGSGADRIAQW
                     RMDVLGQYMRRGRLVKLPRQHAKRWVILEAIAESFSDTRPYTEQEVNTILSAINEDH"
BASE COUNT          765 a         1225 c         1009 g          766 t
ORIGIN      
        1 acccaatttg agcgcgaatt cggcatcctt gtcatcggtg taggtcgccg tgtagaacac
       61 gaagggaacg gcattcaaac ccgggtcaag ccgccactcc cggcagagcg cgtacccgtc
      121 catgaccggc atgaggatat ccgaaacaac cagacgtggc cgctcacgtc gcgcacactc
      181 cagggcggcc tgcccatcag cggcactggc aaccgtatac ccgtgcccgc ccaaaagact
      241 ctcgagataa taccggttgg cctcattgtc atcaaccaca agaatgtcag gcatgcagaa
      301 tcctcaccga acggagtcag gatatcacca aacccgggcg cccgccacga ttttctgata
      361 ttcccggact ttcgagacgt ttcatgcccc gctttcctgc gtacatttgc aggatgaata
      421 cagatccagt catcaaggca tacgccatca caaaacgcta cgggacactg accgctgttg
      481 acggtctctc gttccaggtg ccgccgggaa cctgcctcgg actcctcgga ccgaacggag
      541 cgggaaaaac cacgatgatg aaaatcatct acgggaaggg aagacggagc gggggtgacc
      601 tgcaggtttt cggatacgac ccgggccggc atgccctgga cgtcaaggcc tgcaccggag
      661 tcgttcccca ggaggacagc ctggacgccg agctggacgt ccgctccaat atgcgtctct
      721 atgcccgctt ctacggcatc aaccgccagg tggccgacaa acggatcgag gagcttctct
      781 ccttcatgga actcggggat aaaaacacgg cccggatccg ggaactctcc ggcgggatga
      841 agcgtcgact ggtgatcgcc cgggccctcc tcgggaatcc ccggctcctg atcctggacg
      901 aaccgaccac agggctggat ccccaggtca ggcacctgat ctgggacaga cttcgcaagc
      961 tcaggagcag cggaacaacc atcctgctga caacccatta catggacgag gcctatcaac
     1021 tctgcgactc cctgatcatc atggatcacg gaaaggccgt tctcgaaggc aacccgcggg
     1081 agctggtcga gaaaggcatc gagcgctacg cactccaggt ctttgaccag gagaccggtc
     1141 cggacctgcg cacgcgggcc acagacaggg ccatccgctt cgaggaggcc ggcgggatgc
     1201 tctacgccta tgccgaccgc cttggcgatc tcgaggatct ggctggaatc ctcacggccc
     1261 gcgattatgt tctccggcaa accaatctcg aggatctctt tctccgggca acaggcaagg
     1321 ggttgaacga tgcacagtga cggattccac gcgcaggaca caccgggcga cacggccctc
     1381 ccgccacacc cggccggacc atcacacacg gcaaccggaa ggctggcacc ctcctggcgg
     1441 cagcgaattt ttgccgtctg gttccgccat atgcgggtct acaccaaaaa cctgatcgcc
     1501 aacggctttc ctccgttttt cgagccactc ctctttttgg tcgcgatggg cgtaggattt
     1561 tcgggtgcgt tttcaggaaa gcccgtcgag ggactgcctt ttctggattt tttggccagc
     1621 ggtctcatcc tgacgtcggc catgtacaca tccgccttcg aatgctccta cgggaccttt
     1681 atccggctga attatgataa aacctatgac agcatgctgg ccgcccccct cctcccgcgg
     1741 gacatactga tcggagaaat cctctgggcc ggcaccaagg gcgcgttctt ctcatccgcg
     1801 gtcctctcgg tcatctccgt cttcgggatg atcgggaccg ccggaagtct gctctctccc
     1861 ctcgtgggat tcatcacagg gctggtcttt gcctcggctt cgctcctggt cacctccttt
     1921 gtccggtaca acatcgacca tttcaacttt tattttaccg gatttctttc tcccctcttc
     1981 tttttttgcg ggattctctt ccctgtctcc cagcttcccg ctcccttgag cagcatcgcc
     2041 gaggcactgc ccctgaccca cctcgtccgt ctggaacggg cggtctgctt cggaaactgg
     2101 ggcccggagc acgtctgggc gcttgtctac gccctggtct tcgtcctggc tgtaggctgg
     2161 ctggccatcc gtcgcctgga aaaacaactg atcgactgac agacgacgct tgtcggtgtc
     2221 tctctttcat agtatttatt caatgcagtg cttctgcaca caaggggatg ttcatgaaac
     2281 gttcgaccgc tgttcttgtc ccgggcttga tcaccatcct gaccctggcc gtcttcggat
     2341 acaaccacac cgccatgagt gtcctttcgg gcggagtctc cgagtggaac ctctggcgga
     2401 accgcgaccc gggtcgcgag atcgacctgt ccgacgccga cctcaagggc cggaacctcc
     2461 ggagtgccaa tttcgagcag gcaatccttg agcaggccaa tctttcggaa gccgatctct
     2521 cgtacgctaa aatgggtttt gccaagttgc gccgggccaa tttcagcaag gccaagcttt
     2581 atcgggccat cctcaatgat tccgatctct ccggggcatc cctgaatgat gcgatcctga
     2641 ccctggccca gctcaacaac gccaatctcg agctctgcaa cctgaccggc gcgctcctca
     2701 gcgaagcctc cctcgagaac agcgacgcaa ccgatgccaa tatgagcggg gcccaactcc
     2761 ccaaggccaa cctgtccgcc tccaaaatga tcggagcgaa cttcaaggga gccaatctcg
     2821 cgggagcggt cttctcccag gccgatctcc gcaacgccaa tttcgcgggc gccaacctca
     2881 agggtgccaa tttcagcgga gccaatctcc agaatgccaa tctggccggc gcccaggtgg
     2941 atgacgcaaa cttcagctgg gcaaaactcc agggtgccca gatcgaccgg aaaaacgaag
     3001 ccatcctccg tgcacgcggt gccctgcagc ttgcccaggt caaatggctc caggccgatg
     3061 acggtgtctt gcgttccggc aacgggcaat aatccggatc aaaaggccgt ttgagacacc
     3121 ccgcagggct tcctctgcgg ggtctttttt ttgatgaaca tcctcaggcc acatccagca
     3181 gttctttcct ctatattgta tttctgccgt tatcgaatta aattgacgtc tccggcatcc
     3241 tctgctatgt ttcgatatat gtcgaatccg aatcaggagg ctgatcatgc ccgaaaacga
     3301 atatctggcg gtcctgcgcg ccctggccga cagcacccgt ctccggatgc tgcggactct
     3361 cggctcctgc ccatccagcg ccgaggacct ggccaccctg ctcgggctgg cgccatccac
     3421 ggtctccttc catctcaaaa aactcgaaca ggccggtctt gtgagcggga agcgggaaca
     3481 gtactaccgg atcttttccc gggatgagca agccctcacc ccccggctcc tcgacattgt
     3541 cgacagagag cctgcccgaa gcggatcggg ggcggacagg atcgcccaat ggcgaatgga
     3601 tgttctcggc cagtacatgc gtcgcggccg gcttgtcaaa ctcccgcggc agcacgccaa
     3661 gcgctgggtg attctcgaag ccatcgccga gtcattctcc gacacccggc cctatacgga
     3721 gcaggaggtc aacacgatcc tctcggcgat caacgaggat cactg
//
LOCUS       DAOSHE010000190         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22574_length_3755_cov_3.367838, whole genome shotgun sequence.
ACCESSION   DAOSHE010000190 DAOSHE010000000
VERSION     DAOSHE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22574_length_3755_cov_3.367838"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLD82_06105"
     CDS             complement(<1..682)
                     /locus_tag="PLD82_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64994.1"
                     /translation="MAEKTAGAKSVNKGLAGCMVWLLLGPEKVTKRERVREILAAHGI
                     EDSQGNPARCMFFASDSKPDEIFAEMETYPFFGGKKAVIIQESEKLPRQDLERYLAAP
                     LETTLLVLMSEKPKGKFSAPLERAVDKVGRIEVFWDLFEDRLGPWAENKATREYNLGI
                     PPGFGEFLVDSCGRNMALIEQTIQILANRFPGKGFTLEDAIAVTGEQKGADVFDCIAA
                     CFTGNQVVV"
     gene            complement(755..1453)
                     /locus_tag="PLD82_06110"
     CDS             complement(755..1453)
                     /locus_tag="PLD82_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG64995.1"
                     /translation="MAGCRGRVVRAANKVALALLTVVLAGCAAAPSRHPDFVPDRIKA
                     VYVFSVMAPRDADRVAKRMTGEMSRLLLGQRKVYPVDQISLADGLIEVRIDRLFLEPV
                     SRNADGKIDKARLICDAVFSFRDRSSRAALLENCRLTGVRKVQLVSQPVSDLQAASDQ
                     LADELSTWIIDWCMSGKPPRYNPLFGYEDMPDLDTDGILIGRPRNNRDRNNDGIDDAL
                     QGLTNSSASSQGRW"
     gene            complement(1480..2088)
                     /gene="lexA"
                     /locus_tag="PLD82_06115"
     CDS             complement(1480..2088)
                     /gene="lexA"
                     /locus_tag="PLD82_06115"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOG64996.1"
                     /translation="MKQMTKKQQEVYEFIVARIERQGFPPTVREIGENFEISVKGAYD
                     HLKAIERKGFIKCSSSKSRAIEIMDRIPMASDNMLRVPLMGTTSAGLPILADQNVEEE
                     FYLPKSLLGEQSEVFALRVRGDSMVGAGIHNGDLALVRMQASADDGDIVVALIENEAT
                     IKRFFRQPGGVLLKPENPEYSPMRYPGIDIVGKVVWVFRRIQ"
     gene            complement(2146..3543)
                     /locus_tag="PLD82_06120"
     CDS             complement(2146..3543)
                     /locus_tag="PLD82_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG64997.1"
                     /translation="MADVVQVLVVDDEVHIRETLTGVLEDEGYAAVEAVDGESALQLL
                     ASRRVDIMLLDIWLPGMGGLDVLARVKEQFPAVTVIMISGHGTVNIAVDAIKQGAFDF
                     IEKPLSIDRILMVLEKARKIRTLEEENRSLKGYAVREHALIGASQGMARIRELIVSTG
                     ASNGRVLVTGENGTGKEIIARSIHLASERKDLSFIEVNCAAIPENLIESELFGHEKGA
                     FTGAIEMRKGKFELADRGTIFLDEIADMSLQTQAKVLRVLQEMEFERVGGSKTIHVDV
                     RVIAATNKDLREEIAKGNFREDLFYRLNVIPIHLPALRERLEDMADLVRYFAEQFCRV
                     NGREIVGFSPRAIALLQNHDWPGNIRELRNVVERAIIMLPGKTIEREGLERFFLERNV
                     GPEDEGQAVETGLLEGAYASLKAARDAFEKEYILKKLRENGMNISRTAKILGIERSNL
                     HRKINALGIECDNEG"
     gene            complement(3617..>3755)
                     /locus_tag="PLD82_06125"
     CDS             complement(3617..>3755)
                     /locus_tag="PLD82_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG64998.1"
                     /translation="KTSGGTGLGLSIVEKIVVDHGGKIWFESSAEETVFFMCLPVETM
                     A"
BASE COUNT          773 a         1205 c          940 g          837 t
ORIGIN      
        1 gaacgacaac ctggttcccg gtaaaacagg ccgcgataca gtcaaagaca tccgctccct
       61 tttgttctcc ggtcacagcg atggcatcct cgagcgtaaa cccttttccg ggaaatctgt
      121 tggccagaat ctggatcgtc tgctcgatca gcgccatatt ccgcccgcag gaatccacca
      181 gaaactcccc gaacccgggc ggtatcccca gattgtattc acgtgtggcc ttgttttcgg
      241 cccagggccc gagcctgtcc tcaaaaagat cccagaaaac ttcgatccgg ccgaccttgt
      301 cgacggcccg ctccaggggc gccgagaact ttcccttggg cttttcgctc atcaggacaa
      361 gcagggttgt ctcgagcggt gccgcgagat agcgttccag atcctggcgc ggcaattttt
      421 cggactcctg gatgatgaca gcctttttcc cgccaaaaaa cggataggtc tccatctcgg
      481 caaagatctc gtcgggcttg gagtcactgg caaaaaacat gcaccgtgcg ggattcccct
      541 ggctgtcctc gatcccgtgc gctgcaagga tctccctgac ccgctcgcgc ttggtgactt
      601 tttccggacc gaggagcaac cagaccatgc agcccgcaag gcccttgttg acgcttttcg
      661 ctccggcagt tttttcggcc atcttgctct cccgttgttg tctgcttttt gaggaacacg
      721 cccggatccg caaggtgcgc gcgaacccgc cgggctacca ccgtccctgg ctggacgccg
      781 aggaattggt caacccctgc aacgcgtcat caatcccgtc attgttccgg tcccggttgt
      841 ttctcggcct tccgatcagg attccgtcgg tgtccagatc cggcatatcc tcatatccga
      901 acaacggatt atatctgggt ggtttcccgg acatgcacca atcgatgatc caggtcgaga
      961 gttcatccgc caactgatcg gacgctgcct gaagatcgga gaccggctgg gagaccagct
     1021 ggaccttgcg cacccccgtc aacctgcaat tctcaaggag agcggcccgg ctcgagcggt
     1081 ccctgaaact gaacacggca tcgcaaatca gtcttgcctt gtcaatcttg ccgtcggcat
     1141 tccggctgac cggttcgaga aagaggcggt cgatcctgac ctcgatcagc ccgtcggcca
     1201 gggagatctg atccacggga tagaccttgc gctgcccaag aaggagccgg gacatctcgc
     1261 cagtcatccg cttggccacg cgatccgcat cccggggagc catcacggaa aaaacataga
     1321 cggccttgat ccggtcaggc acaaaatccg gatgccggga gggggcggcc gcacagccgg
     1381 ccagaacaac cgtgagcaga gccagtgcga ccttgttggc cgcacgcaca acacgccccc
     1441 tgcatcccgc cacgatgggc ctcccgtccg tccggaccct cattgaatcc gtcgaaacac
     1501 ccagacaacc ttgccgacaa tatcgatccc cggataccgc atcggggaat actccgggtt
     1561 ttcgggcttg agaagaacgc caccgggttg ccgaaaaaag cgcttgatcg ttgcttcgtt
     1621 ttcgatgagg gcgaccacga tgtctccatc atccgcactg gcctgcatcc ggacgagagc
     1681 caggtcccca ttatggatcc cggcgccgac catgctatcc ccccggacac gcagggcaaa
     1741 gacctcgctt tgctcaccca ggagggattt gggaaggtaa aactcctctt ccacattttg
     1801 gtcggccagg atcggcaacc ccgccgacgt tgtccccatt agcggaaccc ggagcatatt
     1861 gtcactggcc atggggatcc ggtccatgat ctcgatggcg cgggacttgg acgacgaaca
     1921 cttgataaag cccttgcgtt cgatcgcctt gagatggtca taggcaccct tgaccgagat
     1981 ctcgaaattc tcgccgatct ccctgaccgt cggaggaaat ccctgacgct cgatccgggc
     2041 gacaatgaat tcgtagactt cctgctgctt ctttgtcatt tgcttcataa caaatacctc
     2101 taccgctgat tatactacac tcctgtatgc cattcaagcg ctacgttacc cctcgttatc
     2161 gcactcgatc ccgagggcat tgatcttgcg gtgcaaattg ctccgctcga tgcccaaaat
     2221 tttcgctgtc cgactgatat tcatcccgtt ttcacgcagc tttttaagga tgtattcctt
     2281 ctcgaaagca tcccgggccg ccttgagact ggcgtaggcc ccctccagaa gacctgtctc
     2341 gaccgcctga ccctcgtctt ccggccccac gttccgctcc aaaaagaacc gctcgaggcc
     2401 ctcacgctcg atcgtttttc ccggcaacat gatgatcgcg cgttcgacaa cgttccgcag
     2461 ctcccggata tttcccggcc agtcatgatt ctgcaagaga gcaatggccc ggggagaaaa
     2521 cccgacgatc tcgcgcccgt tgaccctgca gaactgttca gcaaaatagc ggacaagatc
     2581 ggccatgtcc tccaaccgtt cccgcagagc aggaagatgg atcgggatca cgttcagtct
     2641 atagaagaga tcctctctga aatttccttt tgcgatctct tcacggagat ccttgttggt
     2701 cgccgcgatc acccggacat cgacgtggat cgtttttgag cccccgacac gctcaaactc
     2761 catctcctgc aggacacgca gcaccttggc ctgcgtctga agcgacatgt ccgcaatctc
     2821 gtcgagaaaa atcgtccccc ggtcggccag ttcgaatttc cccttgcgca tctcgatggc
     2881 accggtaaag gcgccttttt catgcccgaa aagttcactc tcgatcaggt tttcggggat
     2941 cgcggcacaa ttgacttcga taaaagagag atccttccgt tcgctggcaa gatggatcga
     3001 gcgggcgatg atctcctttc ccgtgccgtt ctctcccgta acaagcaccc tgccattgct
     3061 ggcgccggta ctcacgatca attcacggat ccgtgccatg ccctgactgg ctccgatcaa
     3121 ggcatgttcc ctgaccgcgt accccttgag cgatctgttt tcttcttcaa gtgtcctgat
     3181 cttgcgtgcc ttttcaagga ccatcaggat tctgtcgatc gagagtggtt tttcgatgaa
     3241 atcgaaggcc ccctgtttga tcgcatcaac cgcaatattg accgttccat ggccggagat
     3301 catgatgact gtcactgcgg gaaactgctc cttgacccgg gccaagacat ccaggccacc
     3361 catccccggg agccagatat cgagcaacat gatatccacc cgacgggatg ccagaagctg
     3421 gagcgccgat tctccgtcga cggcttccac ggcggcatag ccttcgtctt cgagaacacc
     3481 ggtcagtgtt tccctgatat ggacctcatc gtctacgaca agtacctgta cgacatccgc
     3541 catctctttt gcctccctgc actgcgcctg ttccggtcaa cacgactgcc cgcttgtgct
     3601 ttcgcgcaat ccggtctcaa gccatggttt caaccggcag acacataaag aatacggttt
     3661 cctccgctga actctcaaac cagatctttc cgccatgatc gacgacgatt ttctccacaa
     3721 tcgagagtcc aagaccggtc ccgccgcttg tcttt
//
LOCUS       DAOSHE010000191         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22708_length_3738_cov_4.398860, whole genome shotgun sequence.
ACCESSION   DAOSHE010000191 DAOSHE010000000
VERSION     DAOSHE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22708_length_3738_cov_4.398860"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLD82_06130"
     CDS             complement(<1..252)
                     /locus_tag="PLD82_06130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG64999.1"
                     /translation="MTLNRIALAAIFITAALGGCGNSGFLPEVPADRAPQPADPALSK
                     FTHAVIETSMGDIEVELYWDAAPKTCENFVKLAQKKFYDN"
     gene            468..1538
                     /locus_tag="PLD82_06135"
     CDS             468..1538
                     /locus_tag="PLD82_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG65000.1"
                     /translation="MPGKNDTNASRLIKFRNLELFELYASAAKEVYVRLFDQRETLME
                     TKRALEGYIDKLIDGIDENNRAVFEILATVEFGNRFVSHSLKTTIMSIIIGKQLRFPE
                     GKLFAVGSAAFLHDIGKAKSDNPFLNSYFGAIDDPQDLENNHPIIGASIVAEYLGYGS
                     DIAQGVRNHHEQLDGSGFPRGLNAGSISLMDRVVFTANFIDNLLYRTDYSGIETVSMA
                     IRHAFERFPAKFDPDIKAVLLAFTEKPSISKRRFERTVITLAANYRRWDSGGNYSCRI
                     LDISGSGARIRCKDTMVTGTMLYLNFALSNVMTFNDIVCKVVRRIPDESGYVYGLDFE
                     DKSGAIQQKIDRYVQRYVINQR"
     gene            1603..1676
                     /locus_tag="PLD82_06140"
     tRNA            1603..1676
                     /locus_tag="PLD82_06140"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1637..1639,aa:Asp,seq:gtc)
     gene            complement(2071..2919)
                     /locus_tag="PLD82_06145"
     CDS             complement(2071..2919)
                     /locus_tag="PLD82_06145"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG65001.1"
                     /translation="MQETPSIKAWQVLVLGIGAVSLIQILGRSGVGLVNLLAWAPGTV
                     ESGEFWRLVTHAFVMIDPLSLIFELLFIWFVVPTVEEDWGPGPVLLFFLAASAASAGS
                     AMLLGAIGIRVPFLFGPGAALLGFMYAFSKRNPDQTFLLFFVVPVQAKWFVAVYVAMR
                     AVFSVSQPAMLSLLLAELAGALVSLGLRSLIVSAGKRSETRRRVAAENPDKALAERNA
                     RIEKLVRTKPESDEASRILEAEKNRTFDFTVCPPADFSENDRYCQRCEAFGHCLARSE
                     RHTRQA"
     gene            3145..>3738
                     /locus_tag="PLD82_06150"
     CDS             3145..>3738
                     /locus_tag="PLD82_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG65002.1"
                     /translation="MSGHSKWASIKHKKGANDAKRGQVFTKIIKEISVAARMGGGDLT
                     SNARLRAAVERAKASNMPRDNVERAIKKGTGELEGVSYEELTYEGYGPEGVAIIMDLT
                     TDNKNRTAAEIRKILSRRNGSLGSSNCVAWMFEKKGVINIDCKAIGEDELMELVLEAG
                     AEDMSREGDSYEVLTAPDMFSAVLEALGKKNIPVENAE"
BASE COUNT          802 a         1043 c         1144 g          749 t
ORIGIN      
        1 gttgtcgtaa aacttttttt gggccagctt gacgaaattc tcgcaggtct tgggcgcggc
       61 gtcccagtag agctcgacct cgatgtcgcc catcgaggtc tcgatgacgg cgtgggtgaa
      121 ttttgaaagc gccgggtcgg cgggctgcgg cgccctgtcc gcgggaactt cgggcaaaaa
      181 gccggaattg ccgcagccgc ccagggctgc cgtgatgaag atcgccgcca gggcgatacg
      241 gttgagtgtc atggccaaga tatcctccag atcgagtgtg ggaatctgtt ccccgctaaa
      301 atatacggac ggcggatctc acggaggaat ccgggaattt ttcccctccc tacgggcgaa
      361 gtgctcgcca agagtaacct tcggccggca cccccgccgg actacgcagg ctcgtcggaa
      421 tttgttatac tgacgggaga ggcgattgcc agatcggaga gggagtcatg cccgggaaaa
      481 acgataccaa tgccagtcgc ctgatcaagt tccggaatct tgagcttttt gagctctacg
      541 ccagcgcggc caaagaggtc tatgtccggc tttttgacca gcgcgagacg ctgatggaga
      601 ccaaacgggc tcttgaaggg tatattgaca agctgatcga cggtatcgac gagaacaacc
      661 gggccgtttt cgagattctc gcgacggtcg agttcgggaa ccggttcgta tcgcatagcc
      721 tcaagaccac gatcatgtcg atcatcatcg ggaagcagct ccgcttcccc gagggcaagc
      781 tgtttgccgt gggatccgcc gccttcctgc acgacatcgg caaggccaag agcgacaatc
      841 cctttctcaa ttcctacttc ggggcgattg atgatcccca ggatctcgag aacaaccatc
      901 cgatcattgg agcgagcatc gttgcggagt acctcggcta cggctcggat attgcccaag
      961 gcgtgcgcaa ccaccatgag cagctcgacg gcagcgggtt tccccgcggc ttgaatgcgg
     1021 gttcgatctc gctgatggac cgtgtcgtgt ttaccgccaa cttcatcgac aacctgctct
     1081 accggaccga ctattccggg atagagacgg tctccatggc catccggcac gcgttcgagc
     1141 ggttcccggc caaattcgac ccggacatca aggccgtcct tctggccttc accgaaaagc
     1201 cttcgatctc caagcggcgt ttcgagcgga cggtgatcac ccttgccgcc aattatcgtc
     1261 gctgggacag cggagggaat tattcctgcc gtattctgga tatctcgggg agcggggccc
     1321 ggatccgctg caaggacacc atggtgacgg gaaccatgct ctatctcaat ttcgcgcttt
     1381 ccaacgtgat gaccttcaac gacattgtct gtaaggttgt acgccggatt cccgacgaga
     1441 gcggctatgt gtatggactc gattttgagg acaagtccgg ggcgatccag cagaagatcg
     1501 accgctacgt ccagcgctac gtcatcaatc agcgctgaac aaaaaaaacg taaaaatctt
     1561 ttgaccgggc ggacggcaat ttgtagatta ctggagtcgg ttggaggtgt agctcagctg
     1621 gttagagcgc tggcctgtca agccagaggc cgcgggttca agtcccgtca tctccgatag
     1681 ttcaatcgcc tgcaaaggcg atttttttta tctgccagct tcgcatgcgg gggctgggtt
     1741 tttttgtcct gtcatctgtg tgcagggtat ctgcttttgt actcccgaag tcttcccgaa
     1801 ccgggggagg ggcttgcctt tgttgtggtt tttctggtga acagtctcag gcttggtgtg
     1861 ggcgacgttc cgggcgtgcg aggcaaggct cagtgcgtgc cagacattga acgtgcagca
     1921 gaggatccaa ctgcgtaaaa atttaggcaa aaccgcaatc cgggattgcg gtcgtcatgc
     1981 acgagcgcca gtctcgccca tgtccacaag tctgtggggc cgtctcatgt ttccgcgaag
     2041 cggctgtcag gtttttcctg gtgaacagcc tcaggcctgg cgtgtgtgac gttccgagcg
     2101 tgcgaggcag tggccgaagg cctcacagcg ctggcagtag cggtcgtttt ccgaaaagtc
     2161 cgcaggcggg cagacggtaa aatcaaaagt ccggtttttt tctgcttcga ggatccggct
     2221 ggcctcatcg ctctccggtt ttgtccggac cagtttttcg atccgggcat tccgttctgc
     2281 gagggctttg tccggattct ctgccgcaac ccggcgccgg gtctcggagc gctttccggc
     2341 cgagacaatc agcgaccgga gtccgagcga cacgagggcc cctgcgagtt ccgcaaggag
     2401 gagggagagc atggccggct gggagaccga gaaaacggcc cgcatggcca catagacggc
     2461 gacaaaccat tttgcctgga ccgggaccac aaaaaacaga aggaaggtct ggtccggatt
     2521 gcgcttggag aaggcgtaca tgaatccgag gagggcagcg cccggtccaa aaaggaaggg
     2581 cacccggatg ccgatggctc ccagcaacat cgcacttccg gccgaggcgg cgcttgcagc
     2641 cagaaagaag agcaggaccg gacccggccc ccagtcctct tcgacggtcg gaacgacaaa
     2701 ccagatgaag aggagttcga aaatcaggga gagcggatcg atcatgacga aggcatgggt
     2761 cacaagccgc caaaattcgc cgctttcgac cgtgccgggg gcccaggcca ggaggttgac
     2821 cagcccgaca ccgctccgcc cgaggatctg gatgagggag acggcgccga tccccaaaac
     2881 gaggacctgc caggctttga tggacggcgt ctcttgcatg cgtttcgctc ccctgcaggc
     2941 gtttctggaa taatactgcc ggatggcgcg attctggcag gacacgcgct ttgaaagggc
     3001 ctgaggatgt tcaccaaaaa gacgctgtgt ttgacttgtc tggactgacc cgataccagt
     3061 aaatttccga gcgcgccgcg gatgatggta catgccggcg tccatgcatg cagttgcaag
     3121 cacacttctc aaggagacgt cggtatgtca ggtcactcca aatgggccag tatcaagcac
     3181 aaaaagggcg ccaatgatgc caagcgcggc caggtcttta caaagatcat caaggaaatt
     3241 tcggtcgctg ccaggatggg tggaggggac ctcacctcca atgcgcggct gcgcgccgct
     3301 gtcgaacggg ccaaggcctc caacatgccc cgggacaatg tcgagcgggc catcaaaaag
     3361 gggactggcg agcttgaggg tgtcagctat gaggagctga cctacgaggg ctacgggccc
     3421 gagggtgtcg ccatcatcat ggacctcaca accgacaata aaaaccggac ggccgccgag
     3481 atccgcaaga tcctttcccg ccggaacggg agccttggct cctccaactg cgtggcctgg
     3541 atgttcgaga agaagggcgt gatcaatatt gactgcaagg ccatcggtga ggacgaactg
     3601 atggagctgg ttctcgaagc gggcgccgag gacatgagcc gagaaggcga cagctacgag
     3661 gtcctcaccg ctcccgacat gttttcggcc gtcctcgagg cgctcgggaa aaagaatatc
     3721 ccggtcgaaa atgccgag
//
LOCUS       DAOSHE010000192         3734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_22741_length_3734_cov_4.009242, whole genome shotgun sequence.
ACCESSION   DAOSHE010000192 DAOSHE010000000
VERSION     DAOSHE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22741_length_3734_cov_4.009242"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLD82_06155"
     CDS             complement(<1..654)
                     /locus_tag="PLD82_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG65003.1"
                     /translation="MDWESFFFGRDNYESRRDLAYTSETFNLDKAAELFRLAGNPADP
                     IPKIHVAGTNGKGSTCLYLEQIALASGHTTGVFLSPHVEHPGERIRINGINADEAQLE
                     QAATFLKNCIPDSTRPTTFELLFLAALFLFEEAGCDLVILETGVGGRLDTTNIIRPVV
                     SVIAPIDLDHMALLGPDLASIAAEKAGIIKPGVPLVQAVQQPEADRILKATCTRLGVP
                     "
     gene            complement(654..1286)
                     /gene="udk"
                     /locus_tag="PLD82_06160"
     CDS             complement(654..1286)
                     /gene="udk"
                     /locus_tag="PLD82_06160"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG65004.1"
                     /translation="MPILILIAGGTCSGKTTIARQLGKRLPEGESLIFSHDNYYHDLG
                     HLSQDEIDGYNFDIPDAINADQLVSDVEALLAGRPVTLPRYDFIAHRRTDGITITSPQ
                     TLIILEGIFTLHFSRLMALADLKIFVETASDIRLVRRIQRDTHERNFTLDGVLRQYLA
                     TVKPMHETFIEPTRANADIIIPGDRSFEIALRMIDGFLERELRLASEDDA"
     gene            1515..2243
                     /locus_tag="PLD82_06165"
     CDS             1515..2243
                     /locus_tag="PLD82_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG65005.1"
                     /translation="MPLHLGGTPEELAPVAVLPGDPDRAHLIAHEFLESARCYSQVRG
                     LVGYSGIWKGCPLTVQASGMGGPSAAIICEELAMLGVKTIIRIGTCGTLQAGVSAGSL
                     VIANAACACDGASSQLASVAGYAPAASWGVTAALVAAAQASGTAFRVGTVASMDLFYD
                     PRQAVTAGLRSLGVLALEMEAAAVFTVAALRGLQAGAVFAVTDTIEGQVRAERALIDR
                     TVRSMIEVALDAALAHATGAGGQC"
     gene            2237..>3734
                     /locus_tag="PLD82_06170"
     CDS             2237..>3734
                     /locus_tag="PLD82_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65006.1"
                     /translation="MLIPLILAGFFLGTAVILLIVCLRQRGRIRQLVLDTTILSRICD
                     LSTDGASSLVLTPEGKFSRDWARAPILDTLGIDFASINDFESFSKIIFPEDLPRFRGE
                     LAKLMSGQQVSCIFRVRAADGGIRWLRDTVSPERDSASGRIVRLISVVQDVTEEQETL
                     DVLRRVERRYDEMFRNSSDSIFIVRLDQDGNFVYENFNPRHRLVTGIPEDRLVGRTPH
                     ECLPPEIADHVVEHYSRCITADGPVSYEEALDLPSGYQVALTLLVPIRGEDGRIESIV
                     GFSRVITQRKQAEEELRKGREKYRNIFEESPMGIFQSTLDGRFLSVNPEMCRIFGYPD
                     PESMIKEITDIPTQVYASAQDREGLLRRVLESPVSLAEELVFLKRDGTRFPVRLIIKA
                     VRGPDGTVHHFFGHIEDMTEIRRIEDERNRLEEQLRHAQRVEAIGRLAGAVAHDFNNL
                     LSPILGYAELLLATPETVPWAAELTMIRDTASRAAGITRQLLTFSRKQP"
BASE COUNT          700 a         1030 c         1238 g          766 t
ORIGIN      
        1 cgggacgccg aggcgcgtgc aggtggcctt gaggatccgg tcggcttcgg gctgctgaac
       61 ggcctggacg agggggacac ccggcttgat gatccccgcc ttttcggccg cgatcgaagc
      121 caggtccggc ccgagcaggg ccatgtggtc gagatcgatg ggcgcgatga cggagacgac
      181 gggcctgatg atgttggtcg tatcgaggcg gccgcccacc ccggtctcga ggattacgag
      241 gtcacaaccg gcctcttcaa acaaaaagag cgccgccaaa aagagcagct cgaaggtcgt
      301 cgggcgggtg ctgtcaggga tacagttttt gaggaacgtc gcagcctgct caagttgcgc
      361 ctcgtcggcg ttgatgccgt tgatgcggat ccgctcgccg gggtgttcga cgtgggggga
      421 caaaaacacg cccgtcgtgt ggccggaggc gagggcgatc tgctcgagat agagacaggt
      481 cgagcccttc ccgttggttc cggccacgtg gatcttgggg atcgggtctg ccggatttcc
      541 ggcaaggcgg aagagttcgg cggccttgtc cagattgaag gtttcgctgg tgtaggccag
      601 gtcgcggcgg gattcgtaat tgtcgcgccc gaaaaaaaag gactcccagt ccattatgcg
      661 tcgtcctcgc tggccaggcg cagctcgcgt tccaaaaatc cgtcgatcat ccgcagggcg
      721 atctcgaacg agcggtcacc gggaatgatg atgtcggcat tggcccgcgt cggttcgata
      781 aaggtttcgt gcatcggctt gacggtggcc aggtattgtc gcaggacccc gtcgagagta
      841 aagttgcgct cgtgtgtgtc gcgctggatg cggcgcacca ggcggatgtc cgaggcggtt
      901 tcgacaaaga tcttgagatc ggccagggcc atcagccggc taaagtgcag ggtaaagatc
      961 ccctcgagga tgatcagggt ctgcggggat gtgatcgtga tcccgtcagt ccgccggtgg
     1021 gcgataaaat cgtagcgtgg cagggtgacc gggcggcccg ccagcagggc ctcgacatcc
     1081 gagacgagct ggtccgcatt gatggcgtct ggaatgtcga aattgtagcc gtcgatctcg
     1141 tcctgcgaga ggtgcccgag gtcatggtag taattgtcat gcgaaaagat cagggattcg
     1201 ccctcgggca ggcgcttgcc aagctggcgg gcgatggtcg tcttgcccga acaggtgccg
     1261 ccggcgatca ggatcaggat gggcatgcgc tactccccgc ttgacggata ccacaagtat
     1321 agtcaggctc ccggcgttca ggccaatcct cccgtcggac cgcgtgcttg caaggactcc
     1381 cggagtcgca tgacagtatt gcaaaaaact ggtgtgactc cgggagtgtg cctgaagatt
     1441 cgcggtgcat aaagaccggt cctgtgctat agtcaaaatc ggttgtgcca gatgattgac
     1501 cggttggaga aggtatgccg ctacaccttg ggggaacccc cgaagaactt gcaccagttg
     1561 ccgtcctgcc cggagatccc gaccgggccc acctgatagc gcatgagttc cttgagtcgg
     1621 ccaggtgtta cagccaggtt cgcggacttg tgggctatag cgggatatgg aagggttgcc
     1681 cccttaccgt gcaggccagc gggatgggcg gaccgtcggc cgcgatcatc tgtgaagagc
     1741 tggccatgct gggcgtcaag accatcatca ggatcgggac ctgtgggacc ctgcaggcag
     1801 gggtctccgc ggggtccctt gtcattgcca acgctgcctg cgcctgtgac ggggcctcga
     1861 gccagctggc ctccgtggcc gggtacgcgc cagccgcatc ctggggagtg acagccgccc
     1921 tggtggcggc tgcgcaggcc tccgggactg cattcagggt gggaacggtc gcctcaatgg
     1981 atctttttta tgacccgagg caggctgtca cggcgggctt gcgcagcctt ggtgtcctgg
     2041 ctctcgaaat ggaggcggcg gctgtcttta ccgtggccgc cctgcgcggg ctccaggccg
     2101 gggcagtctt tgccgttacc gataccatcg agggccaggt cagggctgag cgggccctga
     2161 ttgaccggac agtacgcagc atgatcgaag tcgcgcttga tgcagcgctg gcccatgcca
     2221 cgggggcggg cggacaatgc tgattccgtt gatcctggcc ggtttttttc tgggaacagc
     2281 ggtcatcctg ctgattgtct gtctgcgcca gcgggggcgg atcagacagc ttgtgcttga
     2341 tacaaccatc ctctcccgga tctgcgacct ttcaaccgac ggggcttcaa gcctggtgct
     2401 gacgccggag ggcaagtttt cccgggactg ggccagggca cccattctcg acaccctcgg
     2461 gattgatttt gcgagtatca acgacttcga atccttttca aaaatcattt tccccgaaga
     2521 tcttccccgg tttcgtggtg agctggcaaa gctcatgtcc ggacaacagg tctcctgcat
     2581 cttccgtgtc cgggctgcgg atggcgggat ccgctggctg agagacactg tttcgcccga
     2641 gcgggattcg gccagtggcc ggattgtccg gctgatcagt gtggtgcagg acgtgaccga
     2701 agagcaggaa acccttgacg ttttgcgccg ggttgagcgc cgctatgacg agatgttccg
     2761 caacagctcg gacagcattt tcattgtccg tctggaccag gacgggaatt ttgtgtacga
     2821 gaatttcaat ccccggcatc ggttggtgac cggtatcccg gaagacaggc tggttggccg
     2881 gactccacac gaatgccttc cgccggagat cgccgatcat gtggtcgaac actacagccg
     2941 ttgcatcacg gcagatggcc cggtcagcta cgaggaggcg ctcgaccttc cttccgggta
     3001 tcaagtcgct ttgaccctgc tggttcccat ccgtggagag gatggacgga tcgagagtat
     3061 tgtcgggttc tcccgggtga ttacccagcg caagcaggcc gaggaggaat tgcgcaaggg
     3121 aagggaaaaa taccggaata tcttcgagga atccccgatg gggattttcc agtcgacttt
     3181 ggacgggcgc tttctctctg tcaatccgga aatgtgccgg attttcgggt atcctgaccc
     3241 ggagtcgatg atcaaggaga tcaccgacat cccgacgcag gtctatgcga gtgcgcaaga
     3301 ccgtgagggg cttttacgcc gggttctcga gagtccggtt tcacttgcgg aggaactggt
     3361 cttcctgaaa agggacggga cccgttttcc ggtccgactg atcatcaagg ccgtgcgtgg
     3421 tccggacgga acggtccacc atttcttcgg gcatatagaa gatatgacgg agatccggag
     3481 gatcgaagac gaacggaaca ggctcgagga gcaattacgg cacgcccaga gggtcgaagc
     3541 catcgggagg ctagccggtg ccgtcgcaca tgatttcaac aatctcctct cgccgattct
     3601 cgggtatgcc gagttgcttt tggctactcc ggagactgtt ccctgggccg ctgaattgac
     3661 gatgatcagg gatacggcat ccagggcggc cggcataaca cgccagttgt tgactttcag
     3721 caggaaacaa ccgg
//
LOCUS       DAOSHE010000193         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23004_length_3702_cov_4.694269, whole genome shotgun sequence.
ACCESSION   DAOSHE010000193 DAOSHE010000000
VERSION     DAOSHE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23004_length_3702_cov_4.694269"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..463
                     /locus_tag="PLD82_06175"
     CDS             149..463
                     /locus_tag="PLD82_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOG65007.1"
                     /translation="MFVAICIAEVSIPESGSLKDKRRVVRSLKDQIRRDFNVSIAEVE
                     KNEIHSLAVLGITSVCNDPLYLEGQIKHLEEKIDLLSGGMLAWFEHTIERHEPREDWT
                     AG"
     gene            625..2538
                     /locus_tag="PLD82_06180"
     CDS             625..2538
                     /locus_tag="PLD82_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOG65008.1"
                     /translation="MHNDFPAGETEVLEHPSLSHQLIAYIGNKRRLLPLIARALQACK
                     KHHQNQEKTGTPADPVFVDFFAGSGAVSRLAKLLGYHVIANDWEEYSWILNEAFLSIG
                     EEELGGLFAKYGGIENLLSMLNSLETPAPEDCLISRWYAPASTENADPERERMFYTTE
                     NARRIDAIRGWIEHEYPGEILDETATKEKRLLLGLLLYEAATHANTSGVFKAYHRGFG
                     GRGGDALSRILGRIELERPCLSQGSAEACREDAQSLASRLCREGRTARIAYLDPPYNQ
                     HQYGSNYHLLNTIARFDRPKISTLPRTEDGQRRDKAGIRRDWTRTRSPFCSRAAAETA
                     FERLVSTIRAEHILVSYSTEGMIPIHRMVEILGREGRLEIVLAEYTRYRGGKQALTTT
                     TSNIEFVLMAHTGEANTEEDTRRVLQTIGISRLQTLIKRSVSPAAMLDAGYEVRGFAR
                     LDHGLVFERGLACGLRVQVQVDDFKHIAACRFFSDGMEIQPGDMDEARFSAILAEVTA
                     LTSRTREEELEVTMHWVRYLLDTGGIERLVQPMSEIPLLLKKFNDRKAYQASLEYLGK
                     ILELAGEMRRSARPGDLRFARRYQRFAGQLAMIAHRKLAVASRNESAVVRHLRECVRE
                     RSRLMDALASGEC"
     gene            2779..2964
                     /locus_tag="PLD82_06185"
     CDS             2779..2964
                     /locus_tag="PLD82_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65009.1"
                     /translation="MQTRMIMGIVLAVCAVIFIAQNMSDATFKILFITIQMPALIFYV
                     LLLVIGFGLGIVVKSKK"
     gene            2961..>3702
                     /locus_tag="PLD82_06190"
     CDS             2961..>3702
                     /locus_tag="PLD82_06190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG65010.1"
                     /translation="MMRIPLLFALVVWTLALLGILGLSQWLPPRWSMPAAQLLLLSGA
                     LLGARFLLPGRGELRNRLALRPQPAGEVVLAGATAILLLPGLQMLFDRLLRQFSGYAD
                     YVSKQQAVLGGDSHGLGYGFLVVAIVLLPPICEELFFRGYLSSALRSSGYTRAAIILT
                     GGLLFGLFHLDPWRLVPVSILGMIMVWLVETGGSILAGMAFHLVNNGIVFLGSVLAAS
                     TPNAGLDNADLSIGQVFFAAGLFLLGVLT"
BASE COUNT          753 a         1107 c         1052 g          790 t
ORIGIN      
        1 ggtgagccat aacgtgtaaa gacgatgggc atgggtcctc tccgggaaga actgggtatc
       61 ctgtcaagtg tactaccgtg ctctcgcggg ggacaatccg gggagttctc cggatgcacc
      121 cggaccacgc cggccgcgga ggtggatgat gtttgtagcg atctgcattg ccgaggtttc
      181 gattcccgaa tccgggagtc tcaaagacaa acgcagggtt gtccgcagtc tcaaagacca
      241 gatccggcgg gatttcaatg tctcgatcgc cgaggtcgag aaaaacgaga ttcactcgct
      301 ggcggtcctg ggaatcacct cggtctgcaa tgacccgctc tatcttgaag gacagatcaa
      361 gcaccttgaa gagaagatcg atctgctcag cgggggaatg ctcgcctggt tcgagcatac
      421 catcgaacga catgaacccc gcgaggactg gacggctggt tgattcatta cgaaaaccaa
      481 agataaatcc cggattcgca tgacaaatcc gcaatacgac gggagtaccc ggatcgagag
      541 tgtcaagtca cttgcatgaa tgccgatagt caaacagggt ttcccgaaaa cacaagacca
      601 tcccgaggaa caggcgcgtc attcatgcac aacgatttcc ccgcaggaga aactgaggtt
      661 cttgagcatc cctctctctc gcaccaattg attgcctaca tcggcaacaa acggcgcttg
      721 ttgcctttga ttgccagggc gctccaggct tgtaaaaaac accatcagaa ccaggagaaa
      781 acgggcactc ctgccgatcc ggtctttgtg gatttctttg ccgggagcgg cgccgtctca
      841 cgcctggcaa aacttctggg ctaccatgtc atcgccaacg actgggaaga gtattcctgg
      901 atcctgaatg aggccttcct ctcgatcggt gaagaggaac tcggcggcct gtttgccaaa
      961 tatggcggca tcgagaatct cctttccatg ttgaacagcc ttgaaactcc cgcgcccgag
     1021 gactgcctga tcagccgctg gtacgcgccc gcctcgacgg aaaacgcgga tcccgagcgg
     1081 gaacgcatgt tttacacaac ggaaaatgca cgcaggatcg atgcgatccg tggctggata
     1141 gagcacgagt atccggggga gattctggac gagacggcca caaaggaaaa acgtctcttg
     1201 cttggactcc tcttgtatga agccgcaacc cacgccaata catccggcgt ctttaaagcc
     1261 tatcatcgcg ggtttggcgg ccgtggcggg gatgccctct ctcggatcct cggccggatc
     1321 gaactggagc gaccgtgtct gtcacaaggg tcagccgaag cctgccggga ggatgcccag
     1381 tccctcgcca gccgattgtg ccgggaaggc agaaccgcca ggatcgcgta tctggatccc
     1441 ccgtacaatc agcaccagta cggttccaat taccacctgc tgaacacgat cgcccgcttt
     1501 gacaggccca aaatatcgac tcttccccgg acggaagacg gccagcgccg ggacaaagcc
     1561 ggcatccggc gggactggac caggacacgc tccccgttct gcagccgggc ggctgctgaa
     1621 acggccttcg aacggctcgt ctcgacgatc cgggccgaac acatcctcgt ttcctacagc
     1681 acggaaggca tgattcccat ccatcgcatg gtcgagatcc tcggacggga gggacgtctc
     1741 gagatcgtcc tggccgagta cacacgctat cgtggtggaa agcaggcgct gacgacaacg
     1801 accagcaata tcgagttcgt cttgatggcc cataccggcg aagccaatac cgaagaagac
     1861 acccgccggg ttctccaaac catcgggatc tcgcgcctgc aaaccctcat caaacgctcg
     1921 gtcagtccgg ccgccatgct cgatgcgggc tacgaggtgc gcggattcgc ccgtcttgac
     1981 cacggcctgg tctttgaacg cggattggcc tgcgggttgc gcgtccaggt ccaggtcgat
     2041 gacttcaagc acattgccgc ctgccggttt ttttccgacg gaatggagat ccagcccggg
     2101 gatatggatg aagcacgttt ttccgcaatc ctggccgagg tcacagcctt gacctcgcgc
     2161 acccgggaag aagagctcga agtgaccatg cactgggtgc gctacctcct ggataccggc
     2221 gggatcgagc ggctagtcca accgatgagc gagattcccc tcttgctcaa aaaattcaac
     2281 gaccggaaag cctatcaagc ctccctcgag tacctgggca agattctcga actggcgggc
     2341 gagatgcggc gttcggcccg ccccggcgat ctgcggtttg cgcgccggta ccagcgcttt
     2401 gccgggcagc tcgccatgat cgcccaccgc aagctggcag tcgcctcccg aaacgagagt
     2461 gccgtagtgc gccatctccg ggaatgtgtc cgcgagcgca gtcgcttgat ggacgcactg
     2521 gccagtgggg aatgttgaca gaatagtgtt ttctctggtg aacagtctcc agtcccgtca
     2581 tacgccgtcg gatccggaca gaattcgccc atcaacgcca tcctcaggcg ggatgccctg
     2641 cccaaatcgg cgcggatccc gaccggtcca acccgggact cccgtgttgt gacaaacggg
     2701 tgcgtgatcc tcgcaatttt tatggctata ctttgggtgg gctcaggccc tcaatcctct
     2761 tgagtaggga gcagaatcat gcagacacga atgatcatgg gcattgtcct ggctgtgtgt
     2821 gcggtcattt ttatcgcaca aaacatgtcc gacgccacct ttaaaatcct tttcatcacc
     2881 atccagatgc ctgctttgat tttctatgtt ctgttgcttg tcatcggctt cggcttgggt
     2941 attgtcgtca agagcaaaaa atgatgcgca ttcccctgct gtttgccctc gttgtctgga
     3001 cactggcgct cctgggcatc ctggggcttt cccagtggct gccaccccgc tggtccatgc
     3061 cagccgccca gcttctgttg ctgtcagggg cactcttggg cgcccgcttc cttctcccgg
     3121 gaagaggaga actgcgaaac aggcttgccc ttcgtccgca gccagcaggc gaagtggtgc
     3181 tggctggagc aacggccatt cttctgcttc ctggcctgca gatgctcttt gacaggctcc
     3241 tcagacagtt ttcggggtat gcagactatg tcagcaaaca acaggccgtc ctcgggggcg
     3301 acagtcacgg actgggctac ggttttcttg ttgttgccat tgtcctcttg cccccgatct
     3361 gcgaagagtt gttttttcgc ggctacctct cctcggcgct ccggtcctca ggatacacca
     3421 gagctgccat catcctgacc ggcggccttc tcttcgggct gttccacctt gatccctggc
     3481 gtctggtccc cgtctccatt ctgggcatga tcatggtctg gctggtcgag accggcggaa
     3541 gcatcctggc agggatggcc tttcacctgg tcaataacgg gatcgtcttt ttgggctcgg
     3601 tcctcgcggc gagcacgccc aatgccgggc tggacaatgc ggatctctcg atcggacagg
     3661 tttttttcgc tgccgggctg tttttgctgg gagtcctgac gc
//
LOCUS       DAOSHE010000194         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23164_length_3684_cov_3.372279, whole genome shotgun sequence.
ACCESSION   DAOSHE010000194 DAOSHE010000000
VERSION     DAOSHE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23164_length_3684_cov_3.372279"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLD82_06195"
     CDS             <1..592
                     /locus_tag="PLD82_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65011.1"
                     /translation="SIQDDLNPNASLYFRLRATWEINKDHALSVLIAPLRFDASGIAP
                     KAIDYYGKTFAAGEMLEAKYRFDSYRLTYRYTLVDEPTFQFKLGLTAKIRDAAISVRG
                     ETAESERVNTGFVPLVNFSLQWFLGGGLSLLLDGDALAAPQGRAEDILLGIQYDLDGK
                     YFFRGGWRVVEGGADNDKVYNFTWVNYLSLAAGIRL"
     gene            717..2030
                     /gene="miaB"
                     /locus_tag="PLD82_06200"
     CDS             717..2030
                     /gene="miaB"
                     /locus_tag="PLD82_06200"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG65012.1"
                     /translation="MKYFLVTYGCQMNISDSERVAAVLEELGFEKTEHEEEANILGVV
                     ACSVRQTSINRVYGRIVAWNERRDLEQVITFVSGCILERDSKKFAKLFDLQFSIEDLP
                     KLPDLLSTYGVPFVPHEGLASGPDRTGYWKIAPHCESTIQAWIPIQNGCNRFCAYCAV
                     PYTRGREVSRDSQEILDEISGLLERGYKRFTLLGQNVNSYGRDREGSLPVFARLLDDA
                     ASIIEKSGHEALIHFTSPHPSDMSREVFEVMRDRKAVARQVHLPLQSGDDAVLARMRR
                     SYTLARYREIVGWIRDLVPDATLFTDIIVGFPGETEAEFENTRRAMEEFAFDMAFVAM
                     YSPRPGTTGSRMKDDVPQAEKKRRLHVLAELLKEIAGQRKAGMVGTTQRVLVDGLVSP
                     GLWSARTSGLLPVHVAGPEGALATGQFADVRITGSGSMSLAAASI"
     gene            complement(2176..2334)
                     /locus_tag="PLD82_06205"
     CDS             complement(2176..2334)
                     /locus_tag="PLD82_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG65013.1"
                     /translation="MRWICTNCGYIYDPASGDSLGGILPGTAFEDLPEDWVCPVCYAN
                     KDRFDPLD"
     gene            complement(2355..>3684)
                     /locus_tag="PLD82_06210"
     CDS             complement(2355..>3684)
                     /locus_tag="PLD82_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG65014.1"
                     /translation="IRFPDEYLTLGEVPMIRLLLASLCVISLVWAGCSDDDDSGNPSS
                     SGYSLSSSMTSAASVASVSSGASSTVSSLSSSAAPASLFISEYVEGYGNNKYVELYNA
                     TSTALSLSGWSLRTYINGAASPTATLLLSGSVPAGGTFVIRREPTGTWSGTADLTDSG
                     DTMNFNGNDAVGLFNGDTLVDIVGTPGNTSEHIKDMTLVRKPGKAASTTWIADNWYQL
                     PPDTVANLGSHDPVNPMSSSSASSALPVEYPLGSAGNEVYISEYFNGNSSDKFVELFN
                     AGTGQVSLANYRLVRVDVNSDGTTNFANSWCVQLSGSLYKNAAYLFVNGGCNTNTMQT
                     VAGIEEDTGTSGDVRKKTEHPAAYPYGICFFGGNDPIYLVKDGIVVDALGQAQAANWG
                     DFVSIRKKSGRTGNPVWTSDDWVSEIVYIDGPGGGYKEAWPDYTAGWHTP"
BASE COUNT          769 a         1025 c         1074 g          816 t
ORIGIN      
        1 ttccatccag gacgatctca accccaacgc atccctgtat ttccggttgc gagcaacctg
       61 ggagatcaac aaggatcatg cgctttccgt tctgattgca ccgttgcgat tcgatgccag
      121 cgggattgcg cccaaggcga ttgattatta cgggaagact tttgccgcgg gcgagatgct
      181 ggaagcgaag taccggtttg attcgtatcg cctgacgtac cgctacaccc tggtggatga
      241 accgaccttc cagttcaaac tggggttgac ggccaagatc cgggacgcgg ccatctccgt
      301 gcgcggggag acggccgaga gcgagcgtgt gaataccggg tttgttccgc tcgtcaattt
      361 ctccctgcag tggttcctgg gcggcggact ctccctgttg cttgacgggg acgccctggc
      421 cgcaccccaa ggccgggccg aggacattct tctcgggatc cagtacgatc tcgacggaaa
      481 gtatttcttc cgcggaggct ggcgtgttgt cgagggcggc gcggacaatg acaaggtgta
      541 caacttcaca tgggtcaact atctcagtct ggcggccggg atccggctct gaaagaggga
      601 tcggccacca tccagtaccc gtgtggtttc acaggacact ccgatgcggg gtgtcttttt
      661 ttgtcgtgca acggtgctgc gctcacaaat ccgtccgcaa tgcgtatgtt tgagggatga
      721 agtactttct ggtgacctat ggctgtcaaa tgaacatttc ggattccgag cgtgtggccg
      781 cggttctcga agaactcggc ttcgagaaaa cggagcatga agaggaagcc aatattctcg
      841 gtgtcgtcgc ctgctccgtc cggcagacct cgatcaatcg tgtctacggc cggattgtgg
      901 cctggaacga acgtcgcgac ctggaacagg tcatcacatt tgtctcggga tgcatactcg
      961 aacgggacag caagaaattt gcaaaattgt ttgacctgca gttttccatc gaggatctgc
     1021 cgaaactccc ggacctcctc tccacctatg gtgtgccctt tgttccccat gaaggtcttg
     1081 catccggtcc ggatcgcaca ggctactgga aaatagcccc ccattgcgaa tcgaccatcc
     1141 aggcctggat cccgatccag aatggctgca accggttttg tgcctactgc gctgttccct
     1201 acacgcgcgg gcgcgaggtc tcgcgggatt cgcaagaaat tctggacgaa atatccggac
     1261 tccttgagcg ggggtataaa cggttcaccc ttctcgggca aaacgtcaat tcctacgggc
     1321 gtgaccggga ggggagcctg ccggtctttg cacgactcct ggatgacgcc gcctcgatta
     1381 ttgaaaagag cgggcatgaa gcgctgatcc actttacctc gccgcatccg tccgatatga
     1441 gccgcgaggt ctttgaggtc atgcgggacc ggaaagccgt tgccaggcag gtccatctcc
     1501 cgctccagtc cggggacgat gctgttcttg ccagaatgcg ccggtcctac acactggctc
     1561 gctatcgcga gattgtcggc tggatccgcg acctggttcc cgacgcgacc ctctttaccg
     1621 atatcatcgt cgggtttccc ggcgagaccg aggccgagtt cgagaacacg cgccgcgcga
     1681 tggaggagtt cgccttcgac atggcctttg tggcgatgta ttcaccccga cccggaacca
     1741 ccgggagccg gatgaaagac gatgtccccc aggcggaaaa aaagcggcgc ctgcacgttc
     1801 tggcggagct cctcaaggag atcgccgggc aacgcaaggc cgggatggtc gggacaacac
     1861 agcgtgtcct ggttgacgga ctcgtctcgc ccggactctg gtcggcacgg acaagcgggt
     1921 tgttgcctgt tcacgtggcc ggtcctgagg gtgcgctcgc caccggtcag tttgccgacg
     1981 tgcggattac cggctccggt tccatgtcgc ttgccgctgc cagcatctga tttttttgat
     2041 gaacaacctc gggccggaga gtggccggtc gttccgtaac aaaaacgtat tattgtgaaa
     2101 gttgaatatc aggaagatgc ggtccgttaa cgacagtgcg tgttggcatg gcggcaccgg
     2161 tccgggtgga ccggatcaat ccaggggatc aaaccggtcc ttgttggcgt aacagaccgg
     2221 gcagacccag tcttccggca ggtcttcaaa ggcggtcccc ggcaggattc cacccaggga
     2281 gtcgcctgaa gcgggatcgt agatatagcc gcaattggtg cagatccagc gcatcttgtt
     2341 cctcctcaat cggttcaggg tgtatgccag ccggctgtat agtcaggcca agcttcttta
     2401 tacccaccac cggggccgtc gatatacacg atttcactca cccagtcgtc tgaagtccag
     2461 acggggttgc cagtgcggcc tgattttttc ctgatgctga caaaatcacc ccaatttgca
     2521 gcctgtgcct gaccgagagc atcaacaacg ataccatcct tgaccagata gatcgggtcg
     2581 ttcccgccaa aaaagcaaat accatacggg taggctgccg gatgttcggt ttttttcctg
     2641 acatcacctg acgtaccagt gtcttcttca attcctgcca ctgtctgcat cgtgttggta
     2701 ttgcatccgc cattgacaaa gagatatgct gcatttttgt acagactccc gctcaattgg
     2761 acacaccagc tatttgcaaa atttgttgta ccatccgaat taacatcgac ccggaccaaa
     2821 cggtagttgg cgagactgac ttggcctgtt cctgcgttga atagttcgac aaatttatct
     2881 gagctgtttc cgttgaaata ctcactgata tagacctcgt tccctgcaga tccgagcggg
     2941 tactcgaccg gaagcgcgga ggaggcggag ctgctggaca tgggattgac cggatcgtgc
     3001 gatcccagat tggcgaccgt gtcgggaggg agctggtacc agttgtccgc gatccaggtt
     3061 gtggaggcgg ccttgccggg tttccggaca agggtcatat ccttgatgtg ttcgctggtg
     3121 ttgcccgggg tgccgacaat gtcgaccagg gtatcaccgt tgaacagtcc gacggcatca
     3181 ttgccgttga aattcatcgt atccccgctg tcagtcagat cagcagtacc agaccatgtc
     3241 ccggtcggct cgcgtctgat gacaaaggtt ccgcctgcgg ggacggagcc ggagagcagg
     3301 agcgttgctg tcggggaggc tgcgccatta atatacgtcc tgaggctcca gccactgagg
     3361 ctgagtgcag ttgaggtcgc gttgtacagt tcgacatact tgttgtttcc atacccctcg
     3421 acatattccg agatgaagag ggaggcagga gccgcgctgg aggacagcga ggaaaccgtg
     3481 cttgacgctc ctgaagatac tgacgcgaca gaggcggccg aagtcattga cgacgacagc
     3541 gaatatccgg acgaagaagg atttcctgaa tcatcatcat ccgagcagcc cgcccagacc
     3601 aaggatatga cacagagact tgcaagaagc agacgaatca tgggaacctc accaagagtc
     3661 aggtattcat ccgggaaccg gatg
//
LOCUS       DAOSHE010000195         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23362_length_3662_cov_5.153867, whole genome shotgun sequence.
ACCESSION   DAOSHE010000195 DAOSHE010000000
VERSION     DAOSHE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23362_length_3662_cov_5.153867"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1482
                     /locus_tag="PLD82_06215"
     CDS             40..1482
                     /locus_tag="PLD82_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG65015.1"
                     /translation="MLTLGYTKCHGAEEPEDGITVVIGHTNSDLDCFGSMILARLLYP
                     DAILVRSALVHPVARNLHHLYGHRMGMVHVDELDPAKITRIVTVDTRTRNRIDEYFSN
                     LEDFAGEIIVYDHHAGDDGSIPATEIHEHECGSNTTLLGLVVKAQGIVPDEDEATIAL
                     TGIYADTGNFLHNNVTEEDFSVAAWLRACGASMRLVRNFLRPLSDQAQITLFHEVLNS
                     LAYHEIQGHFVVLAELTIEGQLGGLAAIVEKVHEVESPDATFVVFGLKREKSVLVVGR
                     SHRESILVNKVLAPFGGGGHEFAASAYIKGAEASSIHTLLVNALTSDLIPACSAQDVM
                     LFPAHSIQHDWTLLEASMFLERINQTGAAVVDGDGHLAGMLTLRDIMKGRKHAQMHAP
                     VSAYMSRKIVSVGLSTPMRRIEELVHEHNIAHLPVTEDGRVIGIITRDEVIEFIREQS
                     KRERIIAENARAAALELDEKGDEHAARPDP"
     gene            1460..2881
                     /locus_tag="PLD82_06220"
     CDS             1460..2881
                     /locus_tag="PLD82_06220"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOG65016.1"
                     /translation="MPHVLIHDSLTPEDSAMLQALYSRSAASVTSHLDKVRESGSGKF
                     MERFYIGYGHRSIGDCGSTTIFIEGISELAAKAIQDWPLYSGQQTSTRYIDFTRQTLL
                     DPAGTPASRAILDRWMAFYTGRMDAVRDHLAACSPWNPADGDQASWERAIRARSFDVM
                     RAFLPAGVTTQLSWHTNLRQAADKLDLLRYHPLDEVRKLADILREKLAASYPHSFGGK
                     EYPDQEAWREALVAAHTYLDAPWPPASPRQSGLLPAGCASCDWTSLSGNTVQPESGFE
                     LREDGGGLVFASDAQFDRDEIARWIPLLSSRPVKTDLPQTLDSAGLIRTRFVLDYGSF
                     RDLQRHRHGVVRMPLLTARHGFEPWYLAMLPERIRDEALVLCEEQSRAVEALGLSPEK
                     AQYYLAFGYRVVCECAWGLPSAVYVAEMRSGQTVHPTLRRIAQALGTALQAAIPEMAL
                     HIDNKPEAFSLKRGGQDIVEKKQ"
     gene            complement(3061..>3662)
                     /locus_tag="PLD82_06225"
     CDS             complement(3061..>3662)
                     /locus_tag="PLD82_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG65017.1"
                     /translation="LSEYHGMSEEEHAKFLRSLKGKLDRIDTTVSRILTITSLEEEAR
                     ELFTESTDFAAITTEVLERHQQHVAEMGLSITAGFDPAFANYRANHSIIRTVLDELLS
                     NAIYYNKPGGTIDVRGHVKGEYLVLDIIDTGRGIRNEEQEMIFQQFYRTEESDSMNEA
                     GAGLGLFMARSFVERYQGKLSVQSVFGQGSTFTATFLMH"
BASE COUNT          765 a         1126 c         1044 g          727 t
ORIGIN      
        1 tgcttgcatt gcccgccttc atcctctaac attatgcaaa tgctgacact cggatacaca
       61 aagtgccacg gggccgaaga accggaagac ggaatcaccg tcgtcatcgg acataccaac
      121 agcgatctgg attgctttgg ctcgatgatc ctggcccgcc tgctctaccc ggatgcgatc
      181 ctcgtccgga gcgcactggt ccatcccgtg gcacgcaatc tgcaccacct ctacggccac
      241 cggatgggga tggtccatgt cgatgaactg gatcccgcaa aaatcacacg aatcgtcacg
      301 gtcgacacac ggacccggaa ccgcatcgat gagtatttca gcaatctcga ggatttcgcc
      361 ggagagatca tcgtctacga tcaccacgcc ggtgatgatg gttccatccc ggccaccgaa
      421 atccatgagc atgaatgcgg atcgaacacc acactcctcg gactggtggt caaggcgcag
      481 gggatcgttc ccgacgagga cgaagcgacc atcgccctga ccggcatcta cgccgatacc
      541 ggaaattttt tgcacaacaa cgtcaccgaa gaggacttct cggtggccgc ctggctccgg
      601 gcctgcgggg cctcgatgcg gctggtacgg aatttcctgc ggcccctgtc cgaccaggcc
      661 cagatcaccc tcttccacga agtcttgaac agcctggcct accatgagat tcagggacat
      721 tttgtggtcc ttgccgaact cacaattgaa ggccagttgg gtggactcgc ggcgatcgtc
      781 gagaaggtcc acgaagtcga gagccccgat gccaccttcg ttgtattcgg cctcaaacgg
      841 gaaaaatcgg tgcttgtggt cggacgaagc caccgggaat ccattctcgt caacaaggtc
      901 ctggcaccct ttggcggcgg cggccatgaa tttgccgcat cggcctatat caagggcgcg
      961 gaggcctcca gcattcacac gcttctggtc aatgccttga ccagcgatct gattccggcc
     1021 tgttcggccc aggatgtgat gctctttccg gcacactcga tccagcacga ctggaccctg
     1081 ctggaagcct ccatgttcct cgaacggatc aaccagaccg gcgctgccgt ggtggatgga
     1141 gacgggcact tggcgggaat gctcaccttg cgggacatca tgaagggccg aaaacacgcc
     1201 cagatgcacg ccccggtcag tgcctacatg tcccgcaaga tcgtctctgt cggcctctcg
     1261 actccgatgc gacggattga ggagctggtg catgaacaca acatcgccca cctcccggtc
     1321 accgaagacg gccgggtaat cggcatcatc acccgtgacg aggtgatcga gtttatccgg
     1381 gaacagagca aacgggagag aatcatcgcc gagaacgcac gggccgcagc cctcgaactt
     1441 gatgaaaagg gagacgaaca tgccgcacgt cctgatccat gacagcctga cacccgaaga
     1501 cagcgccatg ctgcaagccc tctacagccg gtctgccgcc agtgtgacca gccatctcga
     1561 caaggtccgg gagagcggtt ccgggaaatt catggagcgg ttttatatcg gctatgggca
     1621 tcgctccatc ggagattgcg ggtcaacgac gattttcatc gaaggcattt cggagctggc
     1681 cgccaaggcg atccaggact ggcccctcta cagcggccaa cagacgagca cgcgctacat
     1741 cgactttacc cgacagaccc tgcttgatcc ggccggaacc ccggcctcgc gggcgattct
     1801 cgatcgctgg atggcgttct ataccggccg gatggatgca gtacgtgacc atcttgccgc
     1861 gtgctctccg tggaacccgg ccgacgggga tcaggcctcc tgggaacgcg cgatccgtgc
     1921 ccggagtttt gacgtgatgc gggccttttt acccgcgggc gtgacaaccc agctctcgtg
     1981 gcataccaat ctccgccagg cagccgacaa actggatctc ctgcgctacc accctctgga
     2041 tgaagtccgc aagctggcgg acatcctgcg tgaaaaactc gcggcatcct atccgcattc
     2101 ctttgggggt aaggagtacc ccgaccagga agcctggcgt gaagcactcg tggctgcgca
     2161 tacctatctg gatgcaccat ggccgccggc atcccccagg cagagcgggc ttcttccggc
     2221 cggctgtgcc tcttgtgact ggacctcctt gtccgggaac acggtacaac cggaatctgg
     2281 attcgaactt cgtgaagatg gtggcgggct ggtctttgca tcggatgcac agtttgaccg
     2341 ggatgagatt gcccgctgga tacccctcct ctcttcccgg cctgtcaaaa cggatctacc
     2401 ccagaccctg gattcggccg ggctgatccg gacacgcttt gtgctcgatt acggatcctt
     2461 ccgcgatctg caacgccatc gccacggagt ggtccggatg ccgctcttga cggcacggca
     2521 tgggtttgaa ccctggtatc tggcgatgct gcccgagcgg attcgggacg aagctctggt
     2581 gctgtgtgag gaacagtctc gggccgtgga ggcgctcggt ctgtcacccg agaaagcgca
     2641 gtactatctg gccttcgggt atcgtgtggt ctgcgagtgt gcctggggat taccctcggc
     2701 cgtgtacgtc gccgagatgc gcagcggaca aaccgtccac cccaccctgc gccggatcgc
     2761 acaagcactg gggacagcac tccaggccgc gattccggaa atggcgctcc acatcgacaa
     2821 caaacccgaa gcgttttctc tcaaacgtgg cggacaggac attgtcgaaa aaaagcagtg
     2881 aagcccggaa ccgttcgcac gcgtcccggc gaacacactc cgggattgtt cacaccacaa
     2941 tgacacactg tgtctcgttg cgcgccatcg tccgtcaaac cgccaacgaa gcgacgaacc
     3001 acctgacagt ggcctgagga tgttcatcaa aaaaaagacc tggccgatga gcaaaccgtc
     3061 tcagtgcatc aggaatgttg cggtaaaggt gctgccctgg ccaaagacac tctggacaga
     3121 gagtttccct tggtagcgtt cgacaaaact ccgggccata aagagcccca gcccggcgcc
     3181 agcttcgttc atgctgtccg actcttccgt ccggtagaat tgctggaaga tcatttcctg
     3241 ctcttcgttg cggatgccgc gtccggtgtc gatgatgtcc aggacgagat actcgccctt
     3301 gacatgtccg cggacatcga tcgtgccacc cggcttgttg taataaatcg cattggacag
     3361 aagctcgtcc aggacggtgc ggatgatgct gtgattggcc cggtaattgg cgaaggccgg
     3421 atcaaacccg gcggtgatgc tcaaacccat ctccgcgaca tgctgctgat gccgttcgag
     3481 aacctccgtt gtgatggccg caaagtccgt actttcggta aagagttcgc gggcctcttc
     3541 ttcaagcgag gtaatcgtca aaatgcggga aaccgttgtg tcgatccggt ccagcttccc
     3601 tttgagcgaa cgcaggaatt tggcgtgctc ctcttcggac atcccgtggt attcggagag
     3661 ct
//
LOCUS       DAOSHE010000196         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23367_length_3662_cov_3.418630, whole genome shotgun sequence.
ACCESSION   DAOSHE010000196 DAOSHE010000000
VERSION     DAOSHE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23367_length_3662_cov_3.418630"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLD82_06230"
     CDS             complement(<1..1014)
                     /locus_tag="PLD82_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65018.1"
                     /translation="MPGDSYFTGIHVYSWGSIAAGGSASHLVGIYGLIFICYLLLLLV
                     SRLKREDNRLKQLKIRYMLLSIIVIAVMQIMNLPAMYGVDIYPVGSFIFIPLAILAYG
                     ILQHRLLDIRTVLHLTLLWALVTSLSLVLNIAIYTGVRDILVKGSGLESSTILIGWFV
                     INYLYFSRIQPKIDFLFNRRKFDLKKAGIETTGNMLRLRTVDELGRVLAESMQTVLFF
                     RRAALYVRHPASGILSSRNEADVVCPQALAKTIAQERILQMDLVLTDPGFAESRDDLV
                     LLFARLDCEYIVPVARDTALVALLVLKDRKNLKQLNPDEKNFINTILSAVQISLANAR
                     LYQD"
     gene            complement(915..1343)
                     /locus_tag="PLD82_06235"
     CDS             complement(915..1343)
                     /locus_tag="PLD82_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65019.1"
                     /translation="MFNPAAIPPTLSVIIGLGLAMLSVIRGRFRFQNILFALVILWWS
                     LISAAFVLHQICAGDTQTILEIERIIHVVYVFVPVINLLFFHSLLGIKRTWLVAVFSL
                     PALHSVSRCPVTRTSPESMSTVGARSPLAGAPRIWSASTD"
     gene            complement(1569..2699)
                     /locus_tag="PLD82_06240"
     CDS             complement(1569..2699)
                     /locus_tag="PLD82_06240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG65020.1"
                     /translation="MEIPRKRILVIRLSSYGDILLTTPALEYIRKMHPHASIDYVVYS
                     HFSEMVAHHPLVDHVFAFPKKTILGLIRNRRFAQACRLLKDFFHNIRDTYYDSVIDLH
                     SVTDSAIIALACKTRLRACNRRQLLGLLFSTKFDFPLNNEKAELHASAMALASTSKAL
                     RHSGTVPPLPRLSLTIPAFFLHEAKTILDTIRTGSGSIIGINPDASRAFKSWGHANFA
                     RLCDMLYTTYRYPIVLLGTDRALLASIRQAMVAEVHVISGTNPLTAFALVSLCDFFVS
                     NDSGLMHVAAAFEVPQIAIFGHSNFMKFFPLSRNALSVTKNLPCSPCSEKTAKSCPDR
                     VCINTITPEDVFARFAVLHEMLKPEHEGRCACCTHSFVGHQS"
     gene            2913..>3662
                     /locus_tag="PLD82_06245"
     CDS             2913..>3662
                     /locus_tag="PLD82_06245"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOG65021.1"
                     /translation="MGWNTKANGSGTTYTAGQPLVMSQDTILYAKWGKSVSVNFDPNS
                     PTSGSAPSGTVTGNTTLSVPGNPGSLTKTGYTFGGWNTKKDGTGTTYAEGSTLLLLNN
                     MTLFAKWNSAAVAGNGVRITAANAIGGTTISSTCKLYWYITTDKYLANPAAVVESGSF
                     SSVSETKNVDLPAGTYYVWGFIDKDNSGTKSAGDYGAVTMITVEGFEASSMFSMKMYR
                     EALLLACAVTSSSGYIPAKINFPADVTARYVI"
BASE COUNT          894 a          868 c         1117 g          783 t
ORIGIN      
        1 atcctggtag agtcttgcat tggcgagtga aatctggaca gccgagagga tcgtgttgat
       61 gaaatttttt tcatcgggat tgagttgctt caggttcttc ctgtccttga ggacgagcag
      121 ggccacaaga gcagtgtctc gggcaaccgg aacaatgtac tcgcagtcaa gccgggcaaa
      181 gagcaggaca agatcgtccc gcgattcagc aaatccagga tctgtcagaa caagatccat
      241 ctgcagtatc ctctcctgcg cgattgtctt ggccaaagcc tgcgggcaga caacatccgc
      301 ctcgttgcgg ctggagagga tgccggaggc agggtgccgg acgtaaagtg cggccctccg
      361 gaagaagagc acagtctgca tgctttcggc caggacacgt cccaactcgt caacagtccg
      421 caggcgaagc atgtttccgg tcgtctcgat gccggctttt ttcaaatcaa attttcgccg
      481 gttgaacaga aagtcgattt ttggctggat tctgctgaaa tacagataat tgatgacaaa
      541 ccagccaatc aggatcgtgc tcgattccaa tccggaaccc ttgaccagga tgtcgcggac
      601 tcccgtatag atcgcgatgt ttaacacgag ggagagagat gtgaccaggg cccagagcag
      661 ggtcagatgg aggactgtcc tgatgtcgag cagacggtgt tgcaggatgc cgtaggccag
      721 gatcgcgagc ggaatgaaga tgaagcttcc gacaggatag atatcgactc cgtacattgc
      781 gggcagattc atgatctgca tgacggcaat cacgatgatg gagaggagca tgtaacggat
      841 cttgagctgt ttcaaccggt tgtcttcccg cttcagcctg gaaacaagca agaggagcag
      901 ataacagata aagatcagtc cgtagatgcc gaccagatgc gaggcgctcc cgccagcggc
      961 gatcgagccc caactgtaga catggattcc ggtgaagtac gagtcaccgg gcacctggag
     1021 actgagtgca atgctggcag cgaaaaaacc gcaacaagcc aggtgcgttt gatgccaagg
     1081 agcgagtgga aaaaaagcag attgatgacc gggacaaaaa catagacgac atggataatg
     1141 cgctcgatct cgaggatggt ttgggtgtcg ccggcacaga tctggtgcag cacaaaggct
     1201 gcggagatca gggaccacca gagaatgacg agggcaaaga gaatgttttg gaaccggaac
     1261 cggccacgga tgaccgagag catggccagt cccagtccga tgatgaccga gagagtcggt
     1321 gggatggcgg cagggttgaa catcggcgca cgacctcgtc tgacggtatg gtatccgggg
     1381 tgtgttgcct gatggtctgg ttgcgggatc cccaaacaag cctatgcggt gaggacatgc
     1441 ctgtcaagag tctttcagag atgtctatgc ggtgtctgac catctggttc tggatgatga
     1501 acacatccag gccaatcccg tgcgcaactt gccacttcaa tcgctttctg caggagacgg
     1561 tgcagccctc aggactgatg tccgacaaaa gaatgcgtgc aacaggcgca tcgtccctcg
     1621 tgctccggtt tgagcatctc gtggaggacg gcaaaccggg caaagacatc ctcgggggtg
     1681 atcgtgttga tgcagacacg gtcgggacag ctttttgcgg tcttctcgct gcagggggag
     1741 cagggcaggt tctttgtgac ggacagcgca ttccgtgaca gaggaaaaaa tttcatgaaa
     1801 ttggaatgac caaagatggc aatctgtgga acttcgaagg ctgcggcgac atgcatcagc
     1861 ccggaatcgt tggagacaaa aaagtcgcag agggagacca gggcgaaagc ggtcagcgga
     1921 ttggttccgg aaataacatg cacttctgcg accattgcct ggcggatgct ggcgaggagg
     1981 gctctgtccg ttcccaggag aacgatgggg tatcggtacg tggtatacag catgtcgcag
     2041 agccgggcaa agtttgcgtg gccccaggat ttgaatgcac gcgaggcatc tggattgatg
     2101 ccgatgatgg atcccgaccc tgtccggatt gtatccagaa ttgttttcgc ctcgtgaaga
     2161 aagaatgccg ggatggtcag tgagagccgg ggcagggggg ggactgttcc ggaatggcgg
     2221 agtgccttgg acgtagatgc cagggccatg gcagaggcat gaagttccgc tttttcgtta
     2281 ttcaggggga aatcaaattt tgttgaaaac aagaggccga gaagttggcg ccggttacag
     2341 gctcgtaaac gggttttgca tgcaagcgcg atgatggcac tgtcggtgac ggaatgcaga
     2401 tcgatgacgg agtcgtagta tgtgtcccgt atgttatgga agaaatcctt gagaagtctg
     2461 caggcctgcg cgaacctgcg attccggatc agcccgagga ttgttttttt tgggaacgca
     2521 aagacatggt cgaccaatgg atggtgggcc accatctccg aaaaatggga gtagacaacg
     2581 tagtcgatgg atgcgtgggg atgcatcttc ctgatgtact cgagtgccgg tgtcgtcaac
     2641 aggatgtcac cgtacgaact gaggcggatg acgaggattc ttttccgtgg aatctccatg
     2701 ctgacaaaga tacatgtcca ggccaggcaa accgagattc tttcatgatc gccgggaggc
     2761 agggactgtt cagatgtgac tggattgccc ttctacaagg ttgagacaga cagtcaaatc
     2821 ctgtgacaga caatggtgcg cgtttccgtt ttcgtgtttt ggttcgtttc aggggttgtc
     2881 gaatattaca aaaccgatga gcatatctct ttatgggttg gaacaccaag gccaatggat
     2941 cggggacgac ctacactgca gggcagcctc tggtcatgag ccaggatacc atcctttacg
     3001 ccaagtgggg caagagtgtc tccgtcaatt ttgaccccaa ttctccgact tcgggttcgg
     3061 ctccgtccgg gactgttacc ggaaacacaa ctttgtctgt tccgggcaat cccgggagcc
     3121 tgacaaagac gggctatacg tttggtggct ggaacaccaa aaaagacggc accgggacga
     3181 catacgcaga aggcagtacc ttgctgcttt taaacaatat gacgctcttt gccaagtgga
     3241 attcggcagc cgttgccgga aacggtgtcc ggatcacggc ggccaacgcc atcggcggga
     3301 caacaatcag ttcaacctgc aagctctact ggtatatcac gacggacaag tacctggcca
     3361 acccggcagc ggtcgtcgaa tccgggagtt tttcgtcagt cagtgagacg aaaaacgtcg
     3421 acctgcccgc tggtacgtac tacgtctggg gctttatcga caaggacaac agcgggacaa
     3481 agtcagcggg tgactatggt gccgtgacca tgatcactgt cgaagggttt gaggcttcaa
     3541 gcatgttttc gatgaagatg taccgtgagg cgttgttgct ggcatgcgcg gtgacatcca
     3601 gctcgggata cataccggcc aagatcaact ttcccgcaga tgtcactgca cggtacgtga
     3661 tt
//
LOCUS       DAOSHE010000197         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23493_length_3647_cov_3.572105, whole genome shotgun sequence.
ACCESSION   DAOSHE010000197 DAOSHE010000000
VERSION     DAOSHE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23493_length_3647_cov_3.572105"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1254)
                     /locus_tag="PLD82_06250"
     CDS             complement(253..1254)
                     /locus_tag="PLD82_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65022.1"
                     /translation="MRRFRGVLVGLGLVVFGISLAGADARTRSLNILQAHAMDGFRII
                     SEYNALPLEFTSPSGSSIRFSRGTDFMEYIRDTSESGILKSLSTAVHEICHGLTSRKG
                     AQLRMASGTTVSRGGAYLLNGRYLFVPYTGQIIPSHKVKSMIPKSLRTSRFETYVEDD
                     DDDMGTQQEGVYGQLDEWHAYYHGMLTSVDLWTYYWAKPRLTAEECADFFADIDGVLY
                     AHGEFKLYLLACLKYARERAPRAYRDYLANTAFRTVVLELDQAFSSLIARYFAMKPTV
                     IAHMRAQGHDVRDEGDTIWIGETGRGTFRKEWKLLETALSSQPWVREFAAVRSGVWR"
     gene            complement(1312..1632)
                     /locus_tag="PLD82_06255"
     CDS             complement(1312..1632)
                     /locus_tag="PLD82_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65023.1"
                     /translation="MRLMLVQRYVADKRVDEAQARVALQGFLDGLGGTLDQLKKAMES
                     EEYDLLVRLSGVLRSQAAEHCVTEIADAAAALEMAAEAKSGVACSVHFDALARNAADI
                     RQND"
     gene            complement(1801..1876)
                     /locus_tag="PLD82_06260"
     tRNA            complement(1801..1876)
                     /locus_tag="PLD82_06260"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1841..1843),aa:Asn,seq:gtt)
     gene            complement(1928..2527)
                     /gene="recR"
                     /locus_tag="PLD82_06265"
     CDS             complement(1928..2527)
                     /gene="recR"
                     /locus_tag="PLD82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG65024.1"
                     /translation="MDSFSPAFNRLVGLITRMPGIGARTAERLAWYILKSPPQYARQL
                     AAELAGLHEKIIFCSRCGLMTEQDPCVICTSARRDQGLICVVENPGDALRIEATKEFR
                     GVYHVLMGAISPLNRIHPEDLRITELLDRIREGGVREIILATNPTVEGDTTAYYIAEQ
                     CKDLPVQVSRIARGLPVGSDLEYADSLTLTSALSGREKI"
     gene            complement(2530..2847)
                     /locus_tag="PLD82_06270"
     CDS             complement(2530..2847)
                     /locus_tag="PLD82_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOG65025.1"
                     /translation="MFGDIGAMMKMAKEMQGKMQELQTSLQTLRVTGESGAGMVRVTM
                     NGKHEVMSVVIAEEAMSDREMLQGLVHAATNDAVRRVAEEIKTRMNEMTGGLPLPGMP
                     GMF"
     gene            complement(2869..>3647)
                     /locus_tag="PLD82_06275"
     CDS             complement(2869..>3647)
                     /locus_tag="PLD82_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65026.1"
                     /translation="PELEQPAPGAIPVVAPEHRLAPVVRQDVQTVDGLALVQELASQQ
                     SGPNPFLARVLGEIVRAEIVGSLLVFWLLPRSRFHRSSIESSYAETILAFFTARLGVS
                     LRLECRFTDEAVMPPRPAAPQVPEAVVPPRPAAPQVPEAAMPPRPAAPQAPEAAMPPR
                     PAAPQAHEAAMPPRPAAPHAPQVTTQAAAPHAPQVTTQAAAPARPVGMQTQPHSDIVE
                     SVRSSFGGTVLFDEAGVGEDAATTSEWTEDEGLDESFPPD"
BASE COUNT          840 a         1126 c          966 g          715 t
ORIGIN      
        1 aagaccaacg aaccggatac ctgatcagcc tgaaggccaa agaccgccag catcagcaga
       61 acaaacacaa tcatagctgt ccacagattt ctcaacacac acccccgaat ggctcaaagt
      121 acagtcaagc ataccgcaaa taatcgcgga ttgcaattat tgcaagataa tcagagttcg
      181 gtttctacca gctatacgta aaaccatcat gcaatttgag agcacaccga gagtgatata
      241 atggcgtatc gcctaccgcc agacaccgga ccgcacggcg gcaaattcgc gcacccaggg
      301 ctgtgacgag agcgcagtct cgagaagctt ccattccttg cggaaggtcc cccgcccggt
      361 ttcaccgatc cagatcgtat caccctcatc acgaacatca tgtccctggg ccctcatatg
      421 ggcaatgacc gtcggcttca tggcaaaata cctggcaatc agtgacgaaa acgcctgatc
      481 cagttcaagc acaaccgtgc ggaaggccgt atttgcaaga taatcacggt aagctcgggg
      541 agcgcgctca cgggcatact tgagacaggc caggagatag agcttgaatt cgccatgggc
      601 atacagcacc ccgtcaatgt ctgcaaaaaa atcggcgcac tcttcagccg tcagccgggg
      661 ctttgcccaa taatatgtcc agagatccac cgaagtcagc atcccgtggt aataggcatg
      721 ccactcgtcg agctgaccgt agacgccttc ctgctgtgtc cccatgtcgt catcgtcgtc
      781 ctcgacgtag gtctcgaacc gggatgtccg gagcgacttg ggaatcatcg acttgacctt
      841 gtgcgaagga atgatttgtc cggtatacgg gacaaaaaga tagcggccat tgaggagata
      901 ggccccacct cgagaaaccg ttgtccccga tgccattcgc aactgggccc ctttgcgtga
      961 tgtcaaccca tggcagatct catggacggc agtcgaaagc gacttgagga ttccggactc
     1021 gcttgtatcc cggatatact ccatgaaatc ggtcccgcgg gaaaagcgga tgctcgaacc
     1081 cgacggggaa gtaaactcga gcggaagtgc attgtactcg ctgatgatcc ggaaaccgtc
     1141 catggcatgc gcctgcagga tattgagcga tcttgtacgc gcatcagcac ccgcgaggga
     1201 gatcccgaaa acgacaagcc ccagaccgac cagcacaccc ctgaatctcc gcataccgaa
     1261 cctgcctttc cccctcacac cgggaaccac tctgaagtct gtacaacccg atcaatcatt
     1321 ctgccggatg tcggcggcat ttctggccaa agcatcaaaa tggaccgaac aggcaacgcc
     1381 gctctttgcc tcagccgcca tctccaatgc ggccgcagcg tctgcaatct cggtcacaca
     1441 gtgctcagca gcctggctcc ggagcacacc agaaagccgt acgagaagat catactcttc
     1501 ggactccata gcctttttga gctggtcgag cgtgcccccg agtccatcca gaaacccctg
     1561 cagagccact cgcgcctgcg cttcatccac acgtttgtca gcaacatagc gttgcaccag
     1621 catcagccgc atttccctct ccctgcccag tagtgtcgta ttgctacagt gcaacacatg
     1681 ggcatgccct gtcaagcatg aaccaccggg aaaaaagaag cgtatacccg tgcacgatat
     1741 ggaagaggcc agaggatgtt caccaaaaaa aagaaaaccc gaccctgatg cggatcgggt
     1801 tggctccccc ggcaagactc gaacttgcga cccagtggtt aacagccact tgctctaccg
     1861 gctgagctac aggggaatat cgacgcaatg aatctacaca gagtgtggca aagcggcaag
     1921 tgtttcgtca gattttttcc cgcccggaga gggcgctcgt caaagtcaga gaatcggcgt
     1981 actccagatc gcttcccacc ggcaatcccc gggcaatccg ggagacctga accggcagat
     2041 ctttacattg ctcggcaatg tagtaggctg tcgtgtcccc ttccaccgta gggtttgtgg
     2101 caagaatgat ctcccggaca ccgccttcac ggatacggtc aagcagctcg gttatccgca
     2161 gatcttcggg atgaatccgg ttgagcggag agatcgctcc catcaggaca tggtacacgc
     2221 cccggaactc ttttgtggct tcgatccgca gggcatcccc cggattctcg acgacacaaa
     2281 tcaatccctg gtcccgcctg gcagaggtac aaatcacaca gggatcctgt tcggtcatca
     2341 gcccgcaccg gctgcaaaaa ataatttttt catgcaggcc cgcaagctcg gcagcaagct
     2401 gacgcgcgta ctggggtggg cttttcagga tgtaccaagc caaccgttcg gcggtccgag
     2461 ccccgatgcc gggcatcctc gtgatcaatc cgaccagtct gttgaatgcg ggagaaaaag
     2521 aatccatact cagaacatcc ccggcatacc gggcaggggg aggccgccgg tcatctcgtt
     2581 cattcgagtt ttgatctctt cggccacgcg gcgcaccgca tcattcgttg cagcatggac
     2641 cagaccctgg agcatttcgc gatcactcat ggcttcctcg gcaatcacga cagacatgac
     2701 ctcatgcttc ccgttcatcg tcacgcgaac catgccggcc ccggattctc cggtcacacg
     2761 gagggtctgc aggctggtct gcaattcctg catcttgccc tgcatctctt tggccatctt
     2821 catcattgcg ccaatatcac cgaacatccg ttctccttgc catggtactc agtccggggg
     2881 aaaggactca tcgagccctt catcttcagt ccactcactg gtggtcgcag catcctcacc
     2941 tacccccgct tcgtcgaaca aaacggtgcc tccgaacgag gatcgcacag attcaacaat
     3001 atcggaatgg ggttgcgtct gcataccgac cggacgagcc ggtgcagcag cctgtgttgt
     3061 cacctgggga gcgtgcggtg cagcagcctg tgttgtcacc tggggagcgt gcggtgcggc
     3121 gggccgtggc ggcatcgccg cctcatgtgc ttgcggtgca gcaggtcgtg gcggcatcgc
     3181 ggcctcaggc gcttgcggcg cggcgggtcg tggcggcatc gcggcctcag gtacttgcgg
     3241 tgcagcgggt cgcggcggca ccacggcctc aggtacttgc ggtgcagcgg gtcgcggcgg
     3301 cattaccgct tcatcggtaa accggcattc aagccggagt gaaacaccaa gccgtgccgt
     3361 aaaaaaggcc aggatggttt cggcgtagct gctctcgatg ctcgaacgat ggaaccggct
     3421 cctcggaagc aaccagaaca ccaataatga tccgacaatc tccgcccgga cgatctcgcc
     3481 cagcacccgg gccaaaaatg gattcgggcc gctttgttga ctggccaact cttgcaccaa
     3541 ggcaagccca tcgacagtct gcacatcctg tctcaccaca ggcgctagtc gatgttccgg
     3601 cgcaacgacc gggatcgctc ccggagccgg ttgttccaac tcgggcg
//
LOCUS       DAOSHE010000198         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23502_length_3646_cov_4.418825, whole genome shotgun sequence.
ACCESSION   DAOSHE010000198 DAOSHE010000000
VERSION     DAOSHE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23502_length_3646_cov_4.418825"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="raiA"
                     /locus_tag="PLD82_06280"
     CDS             <1..727
                     /gene="raiA"
                     /locus_tag="PLD82_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG65027.1"
                     /translation="MNVIITGRHFNVSNSLKNAIEEKLGKIRFFYDHVLQFHVFLEKE
                     KLNYKADVTFIADGKKFYLQMKAPTISEAIDKLVDKLERQVRKHKERLQCHSREKIAH
                     GEQGGVPSLQYIEVSNKPKAELEVLIQLANSEDESVAFYPSEESSTAVVVFKESDELF
                     TLYGCDPDDKRWYCKHVYLVDEQVDQTRIEDYQPDSLNDVQAVGRLAQGESAILVYQN
                     ADAKKVQAVLRGNGGYEVFVLEK"
     gene            734..1204
                     /locus_tag="PLD82_06285"
     CDS             734..1204
                     /locus_tag="PLD82_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG65028.1"
                     /translation="MRLAERIHPAAIACGFLAGGKQEAIAGLVNLLDRAYGPLDQRAI
                     VRAVLNREELISTGVGGGVALPHARADGVRDVLVALGIAASAVDFDAQDGQAVRIFFM
                     IVGPLDRVFQRNQLEVEAKISRLLKSSGFSEALLGCADAEQVLACIRTYEAGIG"
     gene            1250..1525
                     /locus_tag="PLD82_06290"
     CDS             1250..1525
                     /locus_tag="PLD82_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG65029.1"
                     /translation="MVERTVVVRNYAGVHARPASMLAELASRFQSRVWLVKDEQQVEC
                     DSVLSVMQLAAVCDSTLIIRADGVDEEEAAHAFEQLISDGFGELEKV"
     gene            1473..2123
                     /locus_tag="PLD82_06295"
     CDS             1473..2123
                     /locus_tag="PLD82_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65030.1"
                     /translation="MHSNSLSATGLANSRRYKLPLLVFVILALRVLPVMGYSGSGVSG
                     LSAGLLFDSRDGSLDSRLDACLAVQPGLPPPAFEDVWPERKDPFFAAALSWFIPGLGQ
                     LYVGKPLKGGLYWLIDNTLFWTAILNVAHVDLGLERDIGFRFAIRARENLSSARIWTS
                     VGVGLCYLAFHIYNVLDAADDAEHHNQEILLREMRRDGLSLIVTPESGSLQWSGSF"
     gene            2146..2673
                     /locus_tag="PLD82_06300"
     CDS             2146..2673
                     /locus_tag="PLD82_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65031.1"
                     /translation="MKTLVALLVSMLLVCQTLPLAADEGLTNGITVQLTPETLARLRQ
                     QYRDPFLAGLLSGLYWGLGHFYTKDYTSGSLFMFGDLVFKGLAVGLVLKLKNKYTGDG
                     DDQIRWREMNGTDRSLVIGAIAVWAGVTILSVADAASSANEYNMKNDPLSRFDLGFSA
                     REGAPFVSLGLKMPF"
     gene            2687..3244
                     /locus_tag="PLD82_06305"
     CDS             2687..3244
                     /locus_tag="PLD82_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOG65032.1"
                     /translation="MRGRIVGYVMAGMLLMAGYHTAAASAYLPSRVSLAEVDGKYVLS
                     VSYQGVFPLKVLSAIEDGMRVQVVCEVVIRRQADFALSRDEEVYLAEYVRSIQYNLMD
                     GRYLVVNLNTGARQRIARRSQLLAMLRSPEDLPVIGNAVFSRRGQYYAEARIAIRFGK
                     LYPPFSFLSIMTYESPWVRSGVLQR"
     gene            3298..>3646
                     /locus_tag="PLD82_06310"
     CDS             3298..>3646
                     /locus_tag="PLD82_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65033.1"
                     /translation="MMRSVLQLAGIGCAGLLVSRFLLEGSGIEQTREVRLAGFLASTL
                     LVILLVIAGLRIFRFVRERKRGVPGAMIRIRLASFFVLAGLVPLVLSILFVATMFNTG
                     MDVVYNPDIENSLD"
BASE COUNT          695 a          952 c         1113 g          886 t
ORIGIN      
        1 catgaatgtg atcatcaccg ggagacattt taacgtttca aacagtttga aaaacgccat
       61 tgaggaaaag ctcgggaaaa tccgcttttt ctacgaccac gttctgcagt ttcatgtctt
      121 cctcgaaaag gaaaagctga attacaaggc ggatgtcacc ttcatcgcgg acgggaagaa
      181 attttatctc cagatgaagg ctccgaccat cagcgaagcg atcgacaaac tggtcgacaa
      241 gctggagcgt caggtccgca agcacaagga acggctccag tgtcacagtc gcgagaagat
      301 cgcccatggc gagcagggcg gggttccttc ccttcagtac atcgaagtct cgaacaagcc
      361 caaggccgaa ctcgaggtgc tcatccagct cgccaactcc gaggacgaga gtgtcgcctt
      421 ttatccgagc gaggagagtt cgaccgccgt ggtcgtcttc aaggagagtg atgagctctt
      481 tacgctctac ggatgtgacc ccgatgacaa gcgctggtat tgcaagcatg tctatctggt
      541 ggatgagcag gttgaccaga ccaggatcga ggattatcag ccggattcct tgaatgatgt
      601 ccaggccgtg gggcgtcttg ctcagggcga gtcggcgatc ctggtgtacc agaatgcgga
      661 cgccaaaaaa gtccaggccg tgctccgtgg gaatggcggg tacgaagtct ttgttctgga
      721 gaaataatcg ctcatgaggc ttgcagaacg gattcatcct gcagcgattg cctgcggttt
      781 tctggccggt ggcaagcagg aagcgatcgc cggtttggtc aatctgcttg atcgtgccta
      841 tggcccgctg gatcagcggg ccatagtgcg tgccgttctc aaccgggagg aattgatatc
      901 aacgggtgtt ggcggcggcg ttgccctgcc gcacgcgagg gcggacgggg ttcgggacgt
      961 ccttgtggct ctcgggatag ccgcctcggc tgttgatttt gatgcgcagg atgggcaggc
     1021 ggtacgtata tttttcatga ttgtaggtcc cctggatcgg gtgtttcagc gcaaccagct
     1081 ggaggtcgag gcaaagatct cgaggctttt gaagagcagc gggttcagcg aggcacttct
     1141 gggttgtgcc gatgcggagc aggttctggc ttgcatccgc acctacgaag ccggtatcgg
     1201 gtaggacgac gattggtact tgcagcctgt gcgaatcaag gggtttgaaa tggtcgagag
     1261 gacggtggtt gtccgcaact atgcgggagt gcatgcgcga ccggcctcca tgctggccga
     1321 gctcgcatcc agatttcaat cccgggtatg gcttgtcaag gacgaacagc aggtcgaatg
     1381 tgacagcgtc ttgagcgtga tgcaacttgc tgccgtctgt gattccacac tgatcattcg
     1441 tgccgatggc gtggatgagg aagaggctgc acatgcattc gaacagctta tcagcgacgg
     1501 gtttggcgaa ctcgagaagg tataaacttc cgctccttgt gtttgtgatc cttgccctcc
     1561 gggttctgcc tgtgatgggc tattcgggct cgggtgtctc cggtctctcc gccgggctgc
     1621 tcttcgattc ccgggatgga tcccttgaca gccggttgga tgcctgtctc gctgtccagc
     1681 ccggattgcc tcctcccgcc tttgaggacg tctggcccga acggaaggat cccttttttg
     1741 cggccgcact ttcctggttc attcccgggt tgggccagct ctatgtcggg aaaccgctca
     1801 agggcggact ctactggttg attgacaaca ccctcttctg gacggcgatc ctcaacgtcg
     1861 cacatgtgga tctcggatta gaacgcgaca tcgggttccg gtttgccatc agggcgcgcg
     1921 agaacctcag ctcggccagg atctggacat cggtcggggt cgggctctgc tatctcgctt
     1981 tccatatcta caatgttctt gatgcggccg acgatgcgga acaccacaac caggaaatcc
     2041 ttctgcggga gatgcggcgt gacggcctct ccctgatcgt tacaccggaa tcgggcagtc
     2101 tccaatggtc cggatctttt tgattctctg ctcactggag gacgtatgaa aacactggtt
     2161 gcactccttg tcagcatgct tctcgtctgc cagaccctgc cgttggctgc cgatgaagga
     2221 ttgaccaacg ggataacggt ccagttgact ccggaaaccc ttgccaggct gaggcagcag
     2281 tacagggatc cgttcctcgc cgggcttctg tccggactct attgggggct cggccatttt
     2341 tataccaagg attacacaag cggttcactc tttatgttcg gggatctggt tttcaagggg
     2401 ttggccgtcg gactggtgct caaactgaag aacaagtata ccggagacgg ggacgaccag
     2461 atccgttggc gggagatgaa cgggacggac aggtctctcg tgatcggggc gatcgccgtg
     2521 tgggcgggtg tgacgattct ctccgtggcc gatgccgcgt caagcgccaa tgaatacaac
     2581 atgaaaaatg atccactctc gaggttcgat cttggctttt cggcccgaga gggtgcgccg
     2641 tttgtctcac tcggcctgaa aatgccattc tagcagggag cgtcggatgc gggggcggat
     2701 tgtcggatat gtcatggctg gcatgcttct gatggccggg taccacacgg ccgccgcctc
     2761 ggcgtatctt ccatcacggg tctcgctggc ggaggttgac gggaaatacg tcctctcggt
     2821 ctcctatcag ggggtgtttc ctctcaaggt cctttcggct atcgaagacg ggatgcgggt
     2881 ccaggtcgtc tgcgaagtcg tgatccggcg gcaggcggat tttgcgctct cgcgggatga
     2941 ggaagtctat ctggccgaat atgttcggag tatccagtac aacctgatgg atggccgcta
     3001 cctggttgtc aatctgaata ccggtgccag acagcgcatc gcaagaagga gccaactttt
     3061 ggcgatgctt cgctcaccgg aggatcttcc tgtcatcggg aatgccgttt tttcccgccg
     3121 ggggcagtat tatgccgaag ccaggatcgc catccgtttc gggaaactct atcccccctt
     3181 cagctttctt tccatcatga cctatgagag tccatgggtg cgctccgggg tgctgcagag
     3241 atgaaacatt ttttccgtcg actcttcaga catccggcac cgccgttggt gtatcgtgtg
     3301 atgcgttcag ttctccagct tgccggaatc gggtgtgcag gtttgcttgt ctcccgtttc
     3361 ctgctggagg gctcggggat cgagcagacc cgtgaggtca ggctggccgg ctttctggct
     3421 tcgacgcttc tggtcatcct gctggtgatt gccggcctga ggatcttccg ctttgtccgg
     3481 gaacggaagc ggggcgtccc gggtgccatg atcaggatcc gtctggcttc ttttttcgtt
     3541 ctggccggtc ttgtgccctt ggtcctttca atattatttg tggcaacgat gttcaatacg
     3601 ggtatggatg tagtgtacaa tccggatata gaaaacagtc ttgatc
//
LOCUS       DAOSHE010000199         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23576_length_3637_cov_3.611949, whole genome shotgun sequence.
ACCESSION   DAOSHE010000199 DAOSHE010000000
VERSION     DAOSHE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23576_length_3637_cov_3.611949"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLD82_06315"
     CDS             complement(<1..274)
                     /locus_tag="PLD82_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG65034.1"
                     /translation="MTHEIERWIADWFHAWTGGWIEAGDRIVRRNLVMKQEHTGRVVS
                     ILGDLARAEKLDCTMARLCRISGWLHDVGRFPQFFRYRTFADDVSCS"
     gene            complement(271..987)
                     /locus_tag="PLD82_06320"
     CDS             complement(271..987)
                     /locus_tag="PLD82_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65035.1"
                     /translation="MRVLVWNCSMSFRKKLDAVMRLAPDIAVIGNSEFPDKLYPGSGP
                     KISGSLWFGNDRKSGLGIYSFGKWHIQPHPVHDASVKTLVPVECTNDNTTFLLLAFQG
                     APASGRQGYGKAFHDYQQALTYIEHIRGRTAIFAGEMGEAALSLWNRESVEGGYGDLE
                     QVLAQADLRSCYHAFTRETPGRERTPTLYLGRDMRQGIHTTCCFASTDLLLGLEDVQV
                     GRPETWLDYSEHMPLVVDFA"
     gene            complement(987..1325)
                     /locus_tag="PLD82_06325"
     CDS             complement(987..1325)
                     /locus_tag="PLD82_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65036.1"
                     /translation="MTQSGEGPRLFVHRCAVCGCEWRTPFPPKKGIRVHCTTCHARLQ
                     AGEAAADIRKEARLKGVRDARQEYVGKKQVLYDSVCISCLQPCRVPFPPEEDRVVLCR
                     ACYAKRQEHH"
     gene            complement(1322..1672)
                     /locus_tag="PLD82_06330"
     CDS             complement(1322..1672)
                     /locus_tag="PLD82_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG65037.1"
                     /translation="MTEQRRTSRLGFLKHLIIINGETVGYLEDLTMGGAGLVLTTVPT
                     QGDRLSFTIRFHACLKIPEIALSGYVRWVSVPLADSVRVGVQFGLLDQPSQLVIRRYM
                     GHASRVQHGKDLRS"
     gene            complement(1686..1922)
                     /locus_tag="PLD82_06335"
     CDS             complement(1686..1922)
                     /locus_tag="PLD82_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65038.1"
                     /translation="MTCTVPDEVVVWLAQRLESGIRGFLAQRLVNETPDLHLFLRLGF
                     EERVKELLHQQGQLSDSEIDESWYEIFLASIRRR"
     gene            complement(2091..2510)
                     /locus_tag="PLD82_06340"
     CDS             complement(2091..2510)
                     /locus_tag="PLD82_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65039.1"
                     /translation="MRIDQVKEVLEGLAKGVFVENGSRFWFARDLVEVAGAGEWCRMR
                     SWIRNAMFLCSSGNEAVESHFRAVGRIVFEDGGQLLCVDDVQMTGYACYLAGVAGAGI
                     SSRAALVAMHFYISSEQDSHLVFEPLPEPGCILIPAA"
     gene            complement(2580..3095)
                     /locus_tag="PLD82_06345"
     CDS             complement(2580..3095)
                     /locus_tag="PLD82_06345"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG65040.1"
                     /translation="MKRHLALIGYRGCGKTSVGRRLSKRLGIPWCDTDVRIQETTGKT
                     IPEIFALQGESGFRRIESAVLAEALSGPDLVLATGGGIILAEGNRLLLREHCFVVWLK
                     ASVEVIHQRINGDAGRPALTDLDPLEEIRTVMRIREPLYAGLADLDLDTGIQPFDRVV
                     DTIADFFEKNN"
     gene            complement(3092..>3637)
                     /locus_tag="PLD82_06350"
     CDS             complement(3092..>3637)
                     /locus_tag="PLD82_06350"
                     /inference="COORDINATES: protein motif:HMM:NF027852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HOG65041.1"
                     /translation="RLIRLEGNNHAVRDLVIARQRARVAGAEYFVHGRYVLGENSVRL
                     EVSLRHAPSGEILTNLVSSRRGNHALYEAACDLGERLAGVVPLIGQIVRIQGNEVQIN
                     LGRVHGVEKGTVFQVYSAASFRERWLASLGRTKPGNPVGEITVHQSDEVVSFGEITDK
                     ETFGGITTYQYLLIKPKETGT"
BASE COUNT          847 a         1102 c          925 g          763 t
ORIGIN      
        1 gggaacagct tacgtcatcc gcaaatgtcc tgtaccggaa aaactggggg aaccggccga
       61 catcatggag ccagcccgaa atccggcaga gcctggccat ggtacaatca agtttttcag
      121 cccgtgccag atccccgagg atcgaaacaa cccggccggt atgttcctgc ttcatgacaa
      181 gattccgtcg cacaattcgg tccccggcct cgatccaccc tccggtccat gcatggaacc
      241 agtccgcaat ccagcgttcg atctcgtgcg tcatgcaaaa tcaaccacga gcggcatgtg
      301 ttcactgtaa tcaagccagg tctcagggcg gccaacctgg acatcctcga gccctaaaag
      361 gagatcggtc gatgcgaagc agcaggttgt atggatccct tgtctcatgt cacggcccag
      421 atacagggtc ggagttcttt cccgtccggg tgtctcacgg gtaaacgcgt ggtaacagct
      481 tctcaagtct gcctgcgcca aaacctgttc cagatcaccg taaccaccct cgacagactc
      541 acggttccag agtgacagag cagcctctcc catttctcct gcaaaaatcg ccgtgcggcc
      601 acggatgtgt tcgatgtatg tcagtgcctg ctgatagtca tgaaaagcct ttccgtatcc
      661 ctgccttccg gatgcaggtg caccctggaa agccagaagc aggaacgtcg tgttatcatt
      721 ggtacattcg accggaacaa gggttttgac agatgcatca tgcacgggat gcggttgaat
      781 atgccatttc ccgaacgaat agatccccag tccgcttttt cgatcattgc cgaaccagag
      841 cgatcccgat atcttcggtc cggacccggg atagagcttg tccggaaact cggaattgcc
      901 aatgacagca atgtccgggg caagtcgcat gacggcatcc agttttttcc gaaaggacat
      961 cgagcagttc cagacaagta cacgcatcag tgatgctcct gacgttttgc atagcaggcc
     1021 cgacagagaa ccaccctgtc ttcttccggt ggaaacggaa cacggcaagg ctgtaaacag
     1081 gagatgcaga cggaatcgta caacacctgt tttttcccga catattcctg tctggcatcc
     1141 cgcacgccct tgagacgggc ttccttgcgg atgtccgccg cggcctcgcc cgcctgaagt
     1201 ctggcatggc aggttgtgca atggacacga atgccttttt tggggggaaa cggtgtccgc
     1261 cactcacagc cgcagacggc gcagcgatgg acaaaaagcc ttggaccctc tccggactgt
     1321 gtcatgatct gagatccttt ccgtgctgga cacgggaggc atggcccata tatcgccgga
     1381 tgacaagctg gctcggttga tccagaagtc cgaattgcac accgacccgg acggaatcgg
     1441 ccaaaggaac cgagacccag cgaacatatc ccgaaagagc gatctcgggg attttcagac
     1501 aggcatggaa ccgtatcgtg aaagagagtc ggtccccttg cgtgggaaca gttgtcaaaa
     1561 ccaggcccgc cccgcccatg gtcagatctt cgagataacc aaccgtctcc ccgttgatga
     1621 tgatcaggtg tttgagaaat ccgagccgac ttgtacgacg ctgctctgtc acgaaagtgc
     1681 cttgcctatc tgcgcctgat tgaagccaga aaaatctcgt accaggattc gtcaatctcg
     1741 ctgtcactga gctggccctg ctgatgcaac aattctttga cccgttcttc gaatccaagg
     1801 cgcaaaaaca ggtggaggtc cggagtttcg ttgacaagtc gctgggccag gaaccccctg
     1861 atcccgctct caagacgctg tgcaagccag acgacaacct catcgggaac tgtgcatgtc
     1921 attatccgcc ccttgcaact cgaacaacca gcattctatt ccggattgtc gtcactcttt
     1981 cgcgcaataa taacattgaa cgcgccgcga tgtacaggat tttccacacg gaacattctg
     2041 tcaatgggac cggactggag cgaccggtcc atccggctct ccatccggaa tcatgcggcc
     2101 ggaatgagta tacaacccgg ttcgggcaaa ggctcgaata caaggtggga atcctgttcg
     2161 gaggaaatat aaaaatgcat cgcaaccagt gccgcgcgac tcgaaattcc agcacccgcg
     2221 acaccggcca gataacaggc gtagcccgtc atctggacat catcgacaca aaggagttgt
     2281 ccaccgtctt caaacacgat ccttccaacc gcccggaaat gactctccac cgcctcgttc
     2341 cccgacgagc agaggaacat ggcatttctg atccaggacc gcatccggca ccattccccg
     2401 gcgccagcga cctccaccag atcccgtgca aaccagaaac gggatccgtt ttcgacaaac
     2461 acaccctttg ccaagccttc gagcacttcc ttgacctgat cgatccgcat cggcaagacc
     2521 tcctgacgtt tgtatcctta tcctatccaa acgtccggac cctcccggga ttttcatttt
     2581 cagttatttt tttcaaaaaa atcggcaatc gtgtcaacaa cccgatcgaa aggctggatc
     2641 cctgtgtcca gatccaggtc ggcaagaccg gcatacaacg gctcccgtat acgcatgacg
     2701 gtgcgaatct cttccagcgg atcaagatcg gtcaaagcag gacgacccgc atccccgttg
     2761 atccgttgat gaatgacctc gaccgaagcc ttgagccaga caacgaagca gtgttcccgt
     2821 aaaaggagcc tgtttccttc cgcgagaatg attcccccac cggtggcaag aaccaggtcc
     2881 ggaccggaga gcgcttcggc cagtacagca gactcgatcc ggcgaaaccc cgattcccct
     2941 tgaagggcga agatttcagg aatggttttc ccggtcgtct cctggatcct gacatcggta
     3001 tcacaccacg gtatccccag ccgtttggaa agcctccgtc caaccgaggt cttcccgcaa
     3061 ccacgatatc cgatcaaggc aagatggcgc ttcatgttcc cgtctctttg ggtttgatca
     3121 gaaggtattg gtacgtcgta attccgccaa aggtctcctt gtcggtgatt tctccgaagg
     3181 aaacaacttc gtcgctttga tgaacagtga tctccccgac gggattgccc ggtttggtcc
     3241 tcccgagact ggccaaccaa cgctcccgga atgaggccgc gctgtagacc tgaaagaccg
     3301 tgcccttctc caccccgtgg actcttccca gattgatctg gacttcgttc ccctggatcc
     3361 ggacgatctg cccgatcagg ggaacgactc cggccaggcg ctctccgaga tcacatgccg
     3421 cttcatagag tgcatgattg ccccgccggg aggagacaag attggtcagg atctcaccgc
     3481 tgggtgcatg tctgagactc acttcgagcc ggacggaatt ttcaccgagg acatagcgtc
     3541 cgtggacaaa gtattcggca ccggccaccc gggcccgctg gcgtgcaatc accagatccc
     3601 ggacggcgtg attgtttccc tccagacgga tcagccg
//
LOCUS       DAOSHE010000200         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23663_length_3628_cov_3.891408, whole genome shotgun sequence.
ACCESSION   DAOSHE010000200 DAOSHE010000000
VERSION     DAOSHE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23663_length_3628_cov_3.891408"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLD82_06355"
     CDS             complement(<1..425)
                     /locus_tag="PLD82_06355"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOG65042.1"
                     /translation="MLLEFFLVSLGINAVFFACAALLRTDKVTDLSYSLTFIVLALWS
                     ILRLPAEPTLWQVLLSGMVILWAVRLGGYLVYRIHAMGVDHRFDRMRSKPLVFARFWI
                     LQALTVVAVMLPFALGYDTARADSPWVWLGGAVWLAGLWL"
     gene            complement(444..983)
                     /locus_tag="PLD82_06360"
     CDS             complement(444..983)
                     /locus_tag="PLD82_06360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG65043.1"
                     /translation="MKTWVFALPILLVCSACGSVTQGSVQTSETRNTATSDSMRNLFG
                     TQLKTSSGSSASSSLLAGKVVGIYFSAHWCGPCRQFTPVLIDTYKRLKSQGKPFEIVF
                     VSSDNSEADMYNYMNETGMPWYAVPWKGSIANNLKSKYGVRGIPTLIVVDQNGKTVST
                     SARSEVASMRDQVWSRWVR"
     gene            complement(1130..1486)
                     /locus_tag="PLD82_06365"
     CDS             complement(1130..1486)
                     /locus_tag="PLD82_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG65044.1"
                     /translation="MKDCFTCGDDEHELSRNMAEVLNLYLDTYPENKIIPAPGKKKAG
                     MVEVGVEPHIAFSILLKNARLAHGLSQVETARRLGIENVFSYQRLEKRTPPTFKNLAR
                     VKKLFPEISLDAIFNT"
     gene            complement(1491..1730)
                     /locus_tag="PLD82_06370"
     CDS             complement(1491..1730)
                     /locus_tag="PLD82_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOG65045.1"
                     /translation="MDISGKEMLKLFLNAGWEKKRQAGSHVIVCKGNRTIPVPVHNNA
                     LPEGLCKHLLKILRGLNDEISLQGSPGKAGFYCLV"
     gene            complement(1868..2869)
                     /gene="mvaD"
                     /locus_tag="PLD82_06375"
     CDS             complement(1868..2869)
                     /gene="mvaD"
                     /locus_tag="PLD82_06375"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOG65046.1"
                     /translation="MTEHVWRAAPSLAMIKYWGKAVPAARGAEDSVLESNIPASFSLA
                     VGLEGLHSETRVRLSTDGADRVMLAGREMDPGRYAGFFEGLRRILGVTDHWECRSENN
                     FPTAAGLASSSSGFAALAAACSAASLCDRPEGVHHEQLSEAARIGSASAARAIWPGFV
                     ALQAGARRAEQVHGPDWWPELRVIIVRISDLPKEASSRDAMDATRETSPYYRAWLEDS
                     PRIYARALEALEERDLGRLGPLIRASTMRMFGSMLGADPPVLYWLPQSVAVLQTVARL
                     RREGAQVWETMDAGPQVKLFCLEQEVAGISAALLGALPELEERLLVVRVGGGLTRVG"
     gene            complement(2907..>3628)
                     /locus_tag="PLD82_06380"
     CDS             complement(2907..>3628)
                     /locus_tag="PLD82_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HOG65047.1"
                     /translation="NTAAEAAAPLLEEVSGGRALARILTNESEGRMATASFRLPVRLL
                     RRAGIAGAEMARRIAVFSAVAAADRARAVTHNKGVMNGVSALALATGNDTRAIEAAVH
                     AHAALTGRYLPLTVYRIEGETLVGEIRLPVPLGTASSTGALHPGARLSLAILGAPSAA
                     RLGRIAAALGLAQNLAAMAALVAEGIQAGHMRLHARRTAYLAGARGDLIDEVAGMISR
                     EGMVSLDAARRVIESLRRGEP"
BASE COUNT          774 a         1142 c         1031 g          681 t
ORIGIN      
        1 agccagagtc cggccagcca gacggccccg cccaaccaga cccagggaga atcggccctg
       61 gccgtgtcgt acccgagcgc aaaggggagc atgaccgcga caaccgtgag cgcctggaga
      121 atccagaaac gggcaaagac gagcggtttg cttctcatcc ggtcaaagcg gtggtcaact
      181 cccatcgcat ggatccggta gaccagatag ccgccgagcc gcacggccca gaggatgacc
      241 attccggaaa gcaggacttg ccagagtgtc ggctcggccg gaagacgcag gatggaccag
      301 agcgccagca cgataaaagt cagactgtag gagagatccg tcactttgtc cgtgcgcagg
      361 agtgccgcac acgcaaaaaa gacagcattg attcccagag aaaccagaaa gaattcaagc
      421 agcatacggc accctttccc ggatcagcgg acccagcggc tccagacctg gtcccgcatg
      481 gaagcgacct cgctccgggc cgatgtcgaa accgtcttgc cgttttggtc aacaacgatc
      541 agggtgggga tcccgcgcac accatacttc gacttgagat tgttggcgat gctgcccttc
      601 cagggaacgg cataccaggg cattccggtt tcgttcatat aattgtacat gtctgcttcg
      661 ctgttgtcgg aagaaacgaa gacgatctcg aaaggtttgc cctgggattt gaggcgcttg
      721 taggtatcga tcaggaccgg agtgaactgc ctgcatggac cacaccagtg ggcggaaaaa
      781 tatattccga cgactttgcc agcaagcaat gaagaagaag cggaggagcc ggacgaggtc
      841 ttgagctggg ttccaaagag attccgcatc gagtcggaag tagccgtgtt gcgggtttct
      901 gaagtctgga cgctgccctg cgttaccgaa ccgcaggcag aacacacaag gagaatcggc
      961 aatgcaaata cccatgtttt catcaagaca cactcctgta ttggtatgat accacccatg
     1021 gtaccacaca cttcctgtca aagtatagag tcatttcaac tttttatata taccaagcag
     1081 ggatgacgtc tctcggtatg atctcttttg ggacaactct cattcgttgt caagtgttga
     1141 aaatcgcgtc cagggaaatc tccggaaaca gttttttgac ccttgccaga tttttgaaag
     1201 tcgggggtgt ccttttttcc aaccgctgat aggaaaacac gttttcaatg ccaagacgtc
     1261 tggctgtctc cacctgggag aggccgtgtg caagacgggc attcttcagg agaatactga
     1321 aagctatatg gggttcaacc ccgacctcga ccataccggc ttttttcttt cccggggcag
     1381 ggatgatttt attctcggga taggtatcaa gatacaggtt gaggacttct gccatatttc
     1441 gggagagttc gtgctcgtca tcaccacagg tgaaacagtc tttcaattca tcacaccaag
     1501 cagtaaaacc ctgcttttcc cggactacct tgaagtgata tttcatcatt cagcccccgc
     1561 agaattttaa gaagatgttt gcacaatcct tccggcagtg cgttattgtg tactggtacg
     1621 ggaatggtcc ggtttccctt gcaaacaata acatgagagc cagcttgcct tttcttttcc
     1681 cagccggcat tcaggaagag cttgagcatc tcctttccgg atatgtccac gcaacctcct
     1741 cgtccaaccc ataccacaat actataccaa tacttttggt atgtcaagcc ggaagccttc
     1801 catcatcacc cctgtgatcg gggcgaaagg ctgcggtgtc atacgcgagt cagcgtgcag
     1861 accgtcatca gccgaccctc gtcagcccgc ccccgacccg gaccaccagc aaacgttcct
     1921 ccagttcggg cagggcacca agcagggcgg cagagatccc cgccacttcc tgttcgagac
     1981 aaaagagttt gacctggggc ccggcatcca ttgtctccca gacctgggcc ccctcccgcc
     2041 tgagacgggc gaccgtctgg aggacggcaa ccgactgggg aagccagtag aggaccgggg
     2101 gatccgctcc gagcatggaa ccgaacatgc gcatggtact ggccctgatc aggggaccga
     2161 gccgtcccag gtcacgctcc tccagcgctt caagcgcccg ggcatagatc cggggactgt
     2221 cctccaacca tgcccggtaa tagggactgg tctcgcgcgt cgcatccatg gcatcccggg
     2281 aggaagcctc cttgggcagg tcactgatgc gcacgatgat cacccgcagt tcgggccacc
     2341 agtcgggacc atgcacctgt tccgccctgc gggccccggc ctgcagagcg acaaagcccg
     2401 gccagatggc acgcgctgcc gaagccgatc cgatccgcgc tgcctcggag agttgttcat
     2461 gatgaacacc ctcgggcctg tcacagaggc tggcggcgga acaggccgca gcgagggccg
     2521 caaacccgga cgacgaggag gccagaccgg ctgcggtggg aaaattattt tcgctgcggc
     2581 actcccagtg gtctgtcacc ccgaggatcc tccgcaggcc ttcaaagaat cccgcatagc
     2641 gtccggggtc catctcccgc ccggcaagca tgacccgatc cgcaccatcc gtgctcaatc
     2701 tgacacgggt ttcgctgtga aggccttcca gcccgacggc cagactgaaa ctggccggga
     2761 tattggattc gaggaccgaa tcttccgcac cccgggcggc cggaacggcc ttgccccagt
     2821 acttgatcat cgccagactg ggcgctgccc gccagacatg ttccgtcatg ctccaacctc
     2881 gttccagtgc gtacttggtg ccgggatcat ggctcacccc ggcgcaggga ttcgatgacc
     2941 cggcgcgcgg cgtccagact caccatgccc tcccgggaga tcatgcccgc cacctcgtcg
     3001 atcagatcgc cgcgcgcccc cgcaagatag gcggtgcgcc tggcatgcaa gcgcatatgc
     3061 ccggcctgga tcccctcggc gaccagggcc gccatggccg caaggttctg cgccaacccg
     3121 agagcggccg caatccgtcc caggcgtgct gcggacggcg caccgaggat cgccagagag
     3181 agtctcgcgc cggggtgcag ggcccccgta ctgctggccg tccccagcgg aaccggcaac
     3241 ctgatctccc cgacgagtgt ctccccctcg atccggtaca ccgtcaaggg gagatagcga
     3301 ccggtcagag cggcatgggc gtgcaccgcc gcctcgatcg cccgggtatc gttgcccgtc
     3361 gccagggcca gggcactcac cccgttcatg acacccttgt tgtgtgtcac ggccctggcc
     3421 cggtccgccg cagcgaccgc ggaaaacacc gcgatccggc gggccatctc ggccccggca
     3481 atcccggccc gcctgagaag ccgcaccgga agacggaagg aggccgtcgc catcctcccc
     3541 tcgctctcgt tggtcagaat ccgggcaagg gcccgtccgc ccgagacctc ctcaaggagt
     3601 ggggcggcgg cctcagccgc tgtattgg
//
LOCUS       DAOSHE010000201         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23679_length_3627_cov_3.356943, whole genome shotgun sequence.
ACCESSION   DAOSHE010000201 DAOSHE010000000
VERSION     DAOSHE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23679_length_3627_cov_3.356943"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..459
                     /locus_tag="PLD82_06385"
     CDS             16..459
                     /locus_tag="PLD82_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65048.1"
                     /translation="MFRITYTILLLAFCGFLSADNEEIRPLERAPDISGQWYYGIDSN
                     PEFIVRVTQTPAGVTAHWYIRDPEEGSELAYAEAQGRFMRFHEILMHGRYLRWFQGTP
                     PGERFTAHWIIYAGKTRMQEISMSGNNRITSYLVHGFRPWQSDHP"
     gene            553..1023
                     /locus_tag="PLD82_06390"
     CDS             553..1023
                     /locus_tag="PLD82_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65049.1"
                     /translation="MRTLTLTLFLTILTACAGAVTLEHRMDYSRKGTRSESRHGFLLV
                     NGQAIPDVFSFVQQGDQAWEFFSRRHLWGDDGYHATHVRVTNLHASEPCTSRIMERGW
                     YPGSDRLDGTPGDWLFVRWGTNTAWIAPTGISALVTAKTPPKLKRTSQEEMFRD"
     gene            1027..1806
                     /locus_tag="PLD82_06395"
     CDS             1027..1806
                     /locus_tag="PLD82_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65050.1"
                     /translation="MWRQVLTLLTLLLPVALSAQATNPGKPIPDTPGKPSTNSSPTNS
                     SAGFELDRLSLSGSLKKDNWGLYYNMNLGLSWKAGEDNLFTRLRIDNNEVFDTVSLLG
                     KYSFVSLTGPRVHPYAIFVSEIYDGYPDGAGHTAARIQWGGGFNFNLVNTARILLRIE
                     SGIDITHEFSTGLPDLVFWWRSRIKGEMEIIKDIRLGCVFVLSEPFRSGLDHWYDYEF
                     SLAYRISSTLSIVGAVRFYDHRVIESNSDRFIHQISFVYSM"
     gene            1872..>3627
                     /locus_tag="PLD82_06400"
     CDS             1872..>3627
                     /locus_tag="PLD82_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65051.1"
                     /translation="MLRTACITILILLAMASHLAAGKGAVDFCETRVFLENTGKADVA
                     WTVRYRVLSGELHGFYLEGLDRLRVERMFTDKAWALDAAGRRYGLEITSLANNRWDII
                     LANGKGVSQGHVTYMFYFRTDFSSAGYLAPTTAADGRRLAVFNWAPVQWDEADAMDHY
                     TLTLHTPHVLPDVHNIPAYVSNNRFVLTEPWVNTQYLIDYRRDEKGRLSILFHKNKPG
                     NRYHMRVQIYLPLEWFPTLAAVADGEREPDEGSAASNTAAQSKPFTNQDAQDYFARLY
                     RSPEFLIGISLLAIVSFVLAWAKWRSLLAARRGLKDILWQDIEWTSPRLVVSDLTKKG
                     KIAKDLTPIEAAFYLGIPVKRIVSALLAAWQAEGRLRILSESPLKVKPLTCAPAGDPH
                     GATLFNAFVDDGELSQTELDGLAGDVVRAIREKAWDADVEATRAWYEARIDKTLKPYI
                     GTAQEEQSSGPRAASEHDGMLPLANRWFLWNRIRTWQDEHDGDWEDDWADDEGFDGDA
                     DDGESPESPEVLPDQALAEHLETTAVSEPFALENDIQLNAENMVNAATDASLASDLSF
                     CHSACHAACHSACHSACHS"
BASE COUNT          762 a         1127 c          965 g          773 t
ORIGIN      
        1 cgatagatat tccgtatgtt ccggatcacg tataccatcc tgctccttgc attctgcgga
       61 ttcctctccg cggacaatga agagatccgc ccgctggagc gtgctcctga catcagcggc
      121 cagtggtact atggaatcga ctccaatccg gagttcattg tccgcgtcac gcagacaccc
      181 gcgggtgtca cggcccactg gtatatccgt gatcccgaag aaggaagcga actcgcctac
      241 gccgaagcac aagggagatt catgcgcttt cacgagatcc tgatgcatgg ccgctacctg
      301 cgctggtttc aagggactcc tcctggcgaa cggtttaccg cccactggat catctatgcg
      361 ggcaagaccc ggatgcagga aatcagcatg tccggcaaca accggatcac cagctatctc
      421 gtccacggat tccggccctg gcaatccgac catccctgaa cagaatttct gcaccaacgc
      481 acggaagatg ccgacgtccc cgagtctgtg tactatagta tgtactgaga gatcaattcc
      541 ggaggccgta ccatgagaac gctgacgctg acccttttcc tgaccatttt gactgcctgt
      601 gccggcgccg tcacactgga gcaccgcatg gactactccc gcaagggcac ccgctcggag
      661 tcacggcacg gttttcttct cgtcaacggt caggctatcc ccgatgtctt ctcctttgtc
      721 cagcagggtg accaggcgtg ggagtttttc tcgcgccgac atctctgggg cgatgacggc
      781 taccatgcga cacatgtccg cgtgaccaat ctgcacgcct ccgagccctg cacaagcagg
      841 atcatggagc gcggctggta tccgggctcg gaccgactgg acgggacccc gggggattgg
      901 ctctttgtcc gctggggaac caataccgcc tggatcgctc cgaccgggat ctccgccctg
      961 gtgaccgcca aaacaccccc gaaactcaag cgcacttcac aggaagagat gttcagggat
     1021 tgaaccgtgt ggcgacaggt cctgaccctt ctgacactgc tgctgcctgt cgcactctcc
     1081 gcgcaggcga ccaatcccgg gaagccgatc cccgacaccc cggggaaacc gtccacaaac
     1141 agctcgccga caaattcttc cgcaggattc gagcttgaca gactctcgct ctccgggagt
     1201 ctgaagaagg acaactgggg tttgtattac aacatgaacc tcggcctctc ctggaaagca
     1261 ggggaagaca atctctttac ccgcctccgg atcgacaaca acgaggtctt tgacaccgtc
     1321 tctcttttgg gaaagtacag ctttgtcagt ttgaccggcc cccgggttca cccctatgca
     1381 atttttgtca gcgaaatcta tgatggctac ccggacggcg caggtcacac ggccgcgcgg
     1441 atccagtggg gcggcggttt caatttcaat ctcgtcaaca cagccagaat cctgctccgg
     1501 atcgaaagcg gcatcgatat cacgcacgaa ttttccacag gactgcccga tctggtgttc
     1561 tggtggcgct cgcgcatcaa gggcgagatg gagattatca aggacatccg cctgggctgc
     1621 gtctttgtcc tctcggaacc atttcgcagc ggtctcgacc attggtacga ctacgaattc
     1681 tcactggcct atcgcatctc cagtaccctc tcgatcgtcg gggccgttcg cttctacgac
     1741 cacagggtca tcgaatcaaa cagtgaccgt tttatccatc agatcagctt tgtctattcg
     1801 atgtaggact actcgatatc aaacacgggt ttgtgctata ctctctctga gtccatcagg
     1861 gagaatatcc tatgctgcga acagcctgca tcaccatcct gatcctgctt gccatggcaa
     1921 gccatcttgc cgccggcaag ggtgctgttg atttttgtga gacacgagtc tttctggaaa
     1981 acaccggaaa ggcagatgtt gcctggacag tccggtaccg tgtgctctcc ggcgaactgc
     2041 acggattcta tcttgaagga ctggaccgcc ttcgcgtcga gcgaatgttc acagacaagg
     2101 cttgggctct cgatgccgcc gggcgcaggt atggtttgga gattaccagc ctggccaaca
     2161 accgctggga catcatcctg gccaacggga aaggtgtctc acagggtcat gtcacctaca
     2221 tgttttattt ccggacggat ttttcgtcgg ccggctatct tgcaccaacc acggccgctg
     2281 atggacgcag actggccgtc tttaactggg caccggtgca atgggatgaa gccgatgcga
     2341 tggatcacta taccctgacc ctgcacacac cgcatgtctt gccggatgtt cacaacatcc
     2401 cggcctacgt ttcgaacaat cgttttgtgc tcacagagcc ctgggtcaac acacagtacc
     2461 tgatcgacta ccgcagggat gaaaaaggtc gcctctccat tctcttccat aagaacaagc
     2521 cgggaaatcg ctatcacatg cgagtccaga tctatctccc tctggaatgg tttccaacac
     2581 tcgccgctgt cgctgatggt gaacgggaac cggatgaggg gagtgctgca tccaatactg
     2641 cggctcagtc aaaaccgttt accaatcagg atgcacagga ctactttgcc cgtctctacc
     2701 gatcaccgga atttctgatc ggtatttctc tcctcgccat tgtgagtttt gtgcttgcct
     2761 gggccaaatg gcgttctctg cttgcggcac gacgcggtct caaggacatc ctctggcagg
     2821 acatcgagtg gaccagcccc cgcctggttg tctccgacct gaccaaaaaa ggcaagattg
     2881 ccaaggacct gaccccgatc gaggccgcct tttatctcgg gatacctgtc aaacggatcg
     2941 tcagcgccct gctcgccgcc tggcaggctg aaggccgcct gcgcatcctc tccgaatccc
     3001 ctctcaaagt caagcccctg acctgtgccc cggccggtga tccgcacggg gccacgctct
     3061 tcaacgcctt tgtcgatgac ggggaacttt cgcaaaccga gctggacggt ctggccggtg
     3121 atgtcgtgcg tgccatccgc gaaaaggcct gggatgccga tgtcgaggcc acccgtgcct
     3181 ggtacgaggc caggatcgac aaaaccctca agccctatat cggtactgcg caggaggaac
     3241 agtcctcagg cccgcgtgct gccagcgaac acgacgggat gctcccgctg gccaatcgct
     3301 ggttcctctg gaacaggatc cgcacctggc aggatgaaca cgatggcgac tgggaggacg
     3361 actgggccga tgacgaaggg tttgacggag atgcggatga cggcgaaagt cccgaatcac
     3421 cagaggtcct ccccgatcag gcgctggccg aacatcttga gaccacagcc gtcagcgaac
     3481 cgtttgccct cgaaaatgac atccagctca atgcggaaaa catggtcaac gccgccaccg
     3541 acgcctccct ggccagcgat ctctccttct gtcactcggc ctgccatgcc gcctgtcact
     3601 ctgcgtgtca ttcggcctgc cactcgg
//
LOCUS       DAOSHE010000202         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23697_length_3625_cov_4.502521, whole genome shotgun sequence.
ACCESSION   DAOSHE010000202 DAOSHE010000000
VERSION     DAOSHE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23697_length_3625_cov_4.502521"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLD82_06405"
     CDS             complement(<1..219)
                     /locus_tag="PLD82_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOG65052.1"
                     /translation="MTNIQELLQASLAVKESLLADTVVHAGMEKAVSLMTTALRSGGR
                     VLFCGNGGSAADAQHFAAELVNRFQRERQ"
     gene            complement(421..849)
                     /locus_tag="PLD82_06410"
     CDS             complement(421..849)
                     /locus_tag="PLD82_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65053.1"
                     /translation="MKRLTMALAGLALLAIVPQKNLSANPDSGALAAVMQVVDRWQPV
                     LDGGFGALAAATDAGVATRALEALYGYGLAIHGEAAALRRAWPQLRRIYALPGLVPFF
                     TRIAPAVGRYTAILDQVEKRFAGQQGVQAALGRVRGLAGR"
     gene            complement(846..3392)
                     /locus_tag="PLD82_06415"
     CDS             complement(846..3392)
                     /locus_tag="PLD82_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65054.1"
                     /translation="MIEFYVNVGWSFILSDTKKFVYSILRMVNLSRRKIGPSRELGVS
                     LGSYASLCMAIPLFGRALHYHEKALGLRGIIGDEWGVAQSLQFLGFCHEWRGEYERGI
                     ECFRRSAETFRRIGDSRELGMALVGLVHCQYYLSRYPQAMKDDREYREMVTRSHDRYG
                     ISDSHVNALKYHIEMGNFEEAAAQAEACSTHSSADGIWFIACVNAIYRGILAIETGDP
                     KTARTRLEEARNLYEKGNFLKQYTVHLYAWRAKAGLDLARRMSQGKERRKALSTLYAE
                     LRQARSRMKAWPTHRSELARFFGEYHELAGHAKRARRWYDEAVKLARKTRRRYEEGKA
                     LHVWGLFERSAGAGPRSTELLMEAWRVYSGIGAAAGVRQVGDLLEIRPEQDQAGRTKI
                     LDRHRLSTIIRLGQDVSSLLDPADLLESIIEKSVEVTGAQRGFLMLYGNDGQKLEVRA
                     ARGFSRKDLGEISSGIVQEVIRQGETIVSGDAALDERYTRFRSVAEHGLKSVLCAPIR
                     LYEKIAGACYLDNPLTSGVFDEEDAQLLGLFMTQAAIALENAELYRNLERKVEERTAE
                     LETALEIQSELNRKLVEASEELKVYTDALEKRDRIIREDLLMARRIQTSILPAQTEAR
                     NGFRVSVSYLPMEEVGGDFYDYCILPDGRLRLLIADATGHGVQAALVTMLVKSVWDGI
                     RQTVGGPGDVLEMLNRETIERFGSLTMFFTCAVVDIDRARGRIDWASAGHPAQYLRTG
                     TEVQLLGHTGMIIGLNADAVYETREAAWKTGSLLLLCTDGILEQCNTMGEEFGEERLR
                     NLLILSGKMSSVGMLDLVGTELEKFMGGSLREDDITMLSVTDTGGAETCL"
     gene            complement(3389..>3625)
                     /locus_tag="PLD82_06420"
     CDS             complement(3389..>3625)
                     /locus_tag="PLD82_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65055.1"
                     /translation="VRIEAQLALCRAWFRKGDWARCETMARKGLALLGEHLPARNFSV
                     GLGIVSLLSIFCIWPFPVFLSEKRRIRTENMPER"
BASE COUNT          779 a         1195 c          935 g          716 t
ORIGIN      
        1 ttggcgttcg cgttggaagc ggttgaccag ctccgccgca aaatgctggg catcggccgc
       61 actcccgccg tttccgcaaa acaggacccg accacccgag cgaagggccg tcgtcatcag
      121 cgaaaccgct ttttccatcc cggcatgtac caccgtatcc gcaagcaggc tttctttgac
      181 tgccagcgat gcctgcaata attcctgtat gtttgtcatt tccgatcctc caaacccgaa
      241 gcacaccgaa agaaaatacc gcttttcggg tacatcaagc aaggaaggag acgcttcagc
      301 acgccagctc acgcccccgc caaggacctc tccccgaaac cagatcgtca aacagaatca
      361 tgaaaaaggc gaagcagaat cccatgccgc ttccggttca tcaccgggac aggccccgtc
      421 tcaccgtccc gccagccccc gcaccctgcc gagagcggcc tgaactccct gttgtccggc
      481 aaaccgcttc tcgacttgat cgagaatggc tgtatagcgg ccgacagccg gggcaatccg
      541 ggtaaaaaac ggaacgagcc cggggagagc atagatccgt cgcaactgcg gccaggcccg
      601 ccgcagggcg gccgcctcgc catggatggc caagccgtag ccatagagcg cctcaagcgc
      661 gcgtgttgcc actccggcgt ccgtagccgc ggccagagca ccaaacccgc catcgaggac
      721 aggttgccag cggtccacaa cctgcatcac tgccgcgagc gccccgctat ccggattggc
      781 tgaaagattc ttttggggaa caatcgccaa caaagccagc cctgccagcg ccattgtcaa
      841 acgtttcaca ggcatgtttc cgcaccccct gtatctgtga cactcagcat ggtaatatcg
      901 tcctcacgaa ggctcccgcc catgaatttc tccaattcgg tcccgaccag atcgagcatc
      961 ccgacggaag acatttttcc ggacagaatc agcaaattcc tcagacgttc ctcgccaaat
     1021 tcttcgccca tagtgttgca ttgttccagg ataccatcgg tgcacagaag gagcagactc
     1081 ccggtcttcc atgcagcctc tcgggtttca tagacggcat cggcgttcaa cccgatgatc
     1141 atcccggtat gccccaaaag ttgcacttcc gttcccgtcc ggaggtattg cgcaggatgg
     1201 cctgcggacg cccagtcaat ccgcccccgg gcgcggtcaa tatccacaac ggcacaggta
     1261 aaaaacatcg tcagcgagcc aaagcgctcg atcgtctccc ggttcaacat ctcgagcaca
     1321 tcccccgggc cgcccactgt ttgccgaatc ccgtcccaga cactcttgac cagcatggtg
     1381 accaacgcag cctggacacc gtgaccggtt gcatcggcga tcaggagcct gagccggcca
     1441 tccggaagaa tacagtaatc gtagaaatcc cctccgactt cctccatcgg gagatagctc
     1501 accgaaacac ggaacccgtt ccgggcctcc gtctgggccg ggagtatgct ggtctggatc
     1561 cggcgcgcca tcaagagatc ttcgcgaatg atcctgtccc gtttttcaag ggcatcggta
     1621 tacaccttga gctcttcgga tgcctcgacc agcttccggt tcagctcgga ctggatctcg
     1681 agagccgtct cgagttcggc ggtcctttct tcgaccttgc gctccaggtt ccggtagagt
     1741 tcggcattct cgagcgcaat cgccgcctgg gtcatgaaga gccccaagag ctgggcatcc
     1801 tcttcatcaa aaacaccact ggtcagggga ttgtccagat agcaggcccc ggcgatcttc
     1861 tcgtacagcc tgatgggtgc gcacaaaacc gatttgagtc cgtgttcggc cacactgcgg
     1921 aaccgtgtat agcgctcgtc cagggccgca tcaccggaga caatggtttc accctgacga
     1981 atgacctcct ggacgatccc cgacgagatc tcgccaagat ccttgcggga aaaaccccgg
     2041 gctgcccgca cctcgagctt ttgcccgtca ttcccgtaga gcatcagaaa cccgcgttgc
     2101 gcccccgtca cctcgaccga cttttcgatg atgctctcga gaagatcggc cggatcgaga
     2161 agcgaactga catcctgtcc cagcctgatg atcgtcgaga gccggtgccg gtccaggatc
     2221 tttgtccgtc cggcctgatc ctgctccggt ctgatttcaa gcaagtcccc cacctgacgc
     2281 acaccggcgg cggccccgat cccggaatag acccgccagg cctccatcag gagttcggtt
     2341 gaccgcggcc ccgcaccggc agatcgctcg aagagccccc agacatggag ggctttgcct
     2401 tcctcataac gacgcctcgt ttttcgtgcc agtttgactg cttcgtcata ccagcgcctg
     2461 gcccgtttgg cgtgcccggc caattcatgg tattccccga aaaagcgggc caattcactc
     2521 cgatgtgtcg gccaagcctt catccgtgag cgggcttgac gcaactcggc atagagggtg
     2581 gagagggcct tccggcgttc cttcccctgc gacatcctcc gggccagatc cagtccggct
     2641 ttggcccgcc aggcgtagag atgaaccgtg tactgtttga gaaaatttcc tttttcgtac
     2701 agattccggg cttcctccag ccgggtccgg gctgtcttgg ggtctccggt ttcaatggcc
     2761 agaatccccc tgtagatcgc attcacacag gcgatgaacc agatcccgtc ggcgctggaa
     2821 tgcgtactgc acgcttccgc ctgggcggcc gcctcctcaa agttccccat ctcgatgtga
     2881 tacttgaggg cgttgacatg ggagtcgctg attccatagc gatcgtggga acgggtcacc
     2941 atctcccggt actcccggtc gtccttcatg gcctggggat accgggagag gtagtactgg
     3001 caatggacaa ggcccaccaa ggccatcccc agttcccgtg aatccccgat ccggcggaat
     3061 gtctcggcac tcctcctgaa acactcgatt ccccgttcat actcgccacg ccactcatga
     3121 caaaacccga ggaactggag cgactgggcc acgccccatt catctccgat gatcccccgc
     3181 aacccaaggg ctttttcgtg ataatgcaat gccctcccga aaagaggaat ggccatgcag
     3241 aggctggcat atgatccgag gctgacaccc aactcccggg aaggcccgat cttgcgccgg
     3301 gacagattga ccattcgtaa aatggagtat acaaattttt ttgtatccga aaggataaaa
     3361 gaccagccca cgttgacgta aaactcgatc atctttcggg catattttcc gtcctgattc
     3421 tccgtttttc ggacaaaaac acagggaaag gccagatgca aaaaatggac agcaaactca
     3481 cgatacccag cccaaccgaa aaatttctgg ccggaagatg ttcaccaaga agagccagac
     3541 ccttgcgcgc cattgtctcg cagcgggccc aatccccctt gcggaaccag gcccgacaga
     3601 gtgccagctg tgcctcgatc cgcac
//
LOCUS       DAOSHE010000203         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_23858_length_3607_cov_4.664133, whole genome shotgun sequence.
ACCESSION   DAOSHE010000203 DAOSHE010000000
VERSION     DAOSHE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23858_length_3607_cov_4.664133"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLD82_06425"
     CDS             <1..988
                     /locus_tag="PLD82_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOG65056.1"
                     /translation="RLKEAGALVLGKTVTTEFAYFYPGPTRNPVNPEHTPGGSSSGSA
                     AAVAAGYCSLAVGTQTIGSINRPAAFCGIIGVKPSYNRIPNDGVIPFSKSADHIGFFT
                     QDMCGARIVSSVLCTGWNDALAQKGIGRKPRIAVVSGKFIEQASGESLRVFREDAQAL
                     RELGYDVGELACLEEIDEINALHRDLISFEMAEAHAGWFDTYRHLYRPATVTFIEEGR
                     AVPQARIDLARRRQLEVRQQLQHALDKAGFDLFISPTTPGEAPEGLQSTGSPIMNLPW
                     TFAGMPTITIPSGNRSDKGLPFGLQFSGIFNEDERLIYWVHEIVHRNAGSKS"
     gene            1190..1879
                     /locus_tag="PLD82_06430"
     CDS             1190..1879
                     /locus_tag="PLD82_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65057.1"
                     /translation="MILDEMEKKLRPPELDLKPYPPKVITLADGQKMVVRECQREEIG
                     VLLETVFPLMQVESDYYDIVAARMFAELLGWYRYRVANEFVMVGAIDGEIAGIVNSRL
                     VDGKIGMSLHTMTIRRGLRIGAQLFAAKMEHHLEILGQEEVWIVAESPNGFKRWMIEY
                     ELEDRSAQFPEVRHELGGVPTFVLTRKLYDAVRDQKVTGTRPADPALLKTAEILKAPD
                     KYPQIPGFRRE"
     gene            1997..3202
                     /locus_tag="PLD82_06435"
     CDS             1997..3202
                     /locus_tag="PLD82_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOG65058.1"
                     /translation="MRNVAIVGIGHTGFGNLSSMDLVDVLGFASCSALEDAGILDKRR
                     DLVEQVFVANMGAGRVNSASAVASALVSRIGLEPAMAETIENGPASGASAVKLGMMAI
                     KSGMVDCVLVTGGELMRAVSGWEGTDFVATMLHTEAEYAYGLTLPSFAAMFTRLYMQR
                     YGLTERELAMVAVKDHENGSKNPYAHVKVPCTLEAIHDGENANVVNNIVADPLRLYGM
                     CPVSDGAASLLLCATDSPKMKFFKKQPIRISGVGSATDTQCVHLRKDPLVLDAVRLAA
                     ERAYAMAGLGPKDISFAELHDAFVILELAISEEVGFFERGKSREAVVKGETRIDGRLP
                     INTSGGLKAKGHPLGATGVSQIFELVKQLRGEAEEGRRVTNPKHGLAVNFGGFGNNVV
                     ATICSREDV"
     gene            3199..3594
                     /locus_tag="PLD82_06440"
     CDS             3199..3594
                     /locus_tag="PLD82_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG65059.1"
                     /translation="MNFNFNKYGPKKHEVCAYKCPGCGKLYYPAVMVCDQCHTRRDPS
                     NFYFKDWEKVPLEGDCTLLTWTRVYALPEGYMKKYQDFGIVEFANGLRASGQLDVATP
                     EIGMKLKSSVGVVKERVGKDNYGFIFISP"
BASE COUNT          809 a         1037 c          978 g          783 t
ORIGIN      
        1 ccggttgaag gaagccggcg cgctggtcct cggaaaaacg gtcaccaccg agtttgccta
       61 tttttatccg ggtccgaccc ggaatccggt caacccggag catacacccg gtggttcctc
      121 ctccggttca gcggcagccg ttgctgccgg ttattgctcg ttggccgtcg gaacacagac
      181 catcggatcg atcaacagac cggctgcctt ctgcggcatc atcggggtta aacccagtta
      241 caatcgcatc ccgaatgatg gggtcattcc gttttcaaaa tcagccgacc acatcggatt
      301 ttttacccag gatatgtgcg gagccaggat tgtctccagc gtcctgtgta ccggctggaa
      361 tgatgccctg gcccaaaaag gaatcggacg caagccacgc attgcggtcg tttcaggaaa
      421 attcatcgag caagccagcg gagaatccct ccgggtcttc cgcgaggatg cccaggcctt
      481 gcgcgagctg ggatacgatg ttggcgagct cgcctgtctc gaagaaattg acgaaatcaa
      541 tgccctgcat cgcgacttga tttctttcga aatggccgaa gcccatgctg gctggtttga
      601 cacctatcgt catctctacc gtccggccac cgtgactttt atcgaagaag gacgtgccgt
      661 cccccaggca cgaatcgatc tggcgcgcag acgccagctt gaggtgcgtc aacagctcca
      721 acacgctctc gacaaagccg ggtttgacct ctttatcagt ccgaccaccc cgggtgaggc
      781 tcctgaaggg ctgcagtcaa ccggaagtcc gatcatgaat cttccatgga cctttgccgg
      841 tatgccaacg atcaccattc cgtccggcaa cagatccgac aagggcctgc ccttcggact
      901 gcaattctcc gggatcttca acgaagatga gcgcctgatc tactgggtcc atgaaatcgt
      961 ccaccggaat gccggctcaa agagctgacc tggcttcagg ctggtttcct gaccgctccc
     1021 gtacgggagc ggttttttgt tgacggattt tcatgcggat ggatcaactg aatcgtcttc
     1081 agacttgcaa ttcattctca agaggtacac aatacccttt gagcagacgg accatccgct
     1141 atgccttttc acagtcggat aatcccggcc gtcccacgga gcgatgacca tgatactgga
     1201 tgaaatggaa aaaaaactga gaccacccga gcttgatttg aaaccctacc ctcccaaagt
     1261 catcaccctc gccgacggac aaaaaatggt tgtgcgtgaa tgccagcgcg aggaaatcgg
     1321 cgtcctgctt gagaccgtct tcccactgat gcaggtcgag tcagattatt atgatattgt
     1381 ggcagcacgc atgtttgctg agcttctggg ttggtatcgc tatcgagtgg ccaacgagtt
     1441 cgtcatggtc ggggccattg atggcgagat tgccggcatc gtcaatagcc gtctggtcga
     1501 tggcaagatc ggcatgagcc tgcacaccat gacgatccgc cgaggactcc gcatcggagc
     1561 gcaactcttt gccgcaaaaa tggagcatca cctcgaaatc ctcggacagg aagaggtctg
     1621 gatcgtcgcc gaaagtccca acggcttcaa gcgctggatg atcgagtacg aactcgagga
     1681 ccgctcggct cagttccctg aagtgcgcca tgaattgggt ggcgtcccga cctttgtcct
     1741 gacacgcaag ctctatgatg cggtccgcga tcaaaaggtt accggcaccc gccccgccga
     1801 cccggccctt ctgaaaactg ccgagattct caaagcgccc gacaagtatc cccagattcc
     1861 gggattccgc agggaataac aacttttttg aggaacagcc tcatgcctcc cgcacaaggg
     1921 ctcccgcgac gcaccgtgtt tcttcggctt gagtgtgttc acaaaaaatt accgccaaga
     1981 tgcagaaagg tctctcatgc gaaatgtagc aatcgttggt atcgggcaca ccgggttcgg
     2041 caacctctcg tccatggatc tggtcgatgt cctgggtttt gcttcctgca gtgccctgga
     2101 agatgcaggc attcttgata aacgccgcga cctggtcgaa caggtctttg tggccaatat
     2161 gggtgctggc cgtgtgaatt cggccagtgc cgtcgcctcg gctctcgtca gccggattgg
     2221 tctcgaaccg gccatggccg aaaccattga aaacggccca gcttcgggag ccagtgccgt
     2281 caagctgggg atgatggcca tcaagagcgg catggtcgac tgcgtacttg tcaccggtgg
     2341 cgaactgatg cgggccgtct cgggctggga aggaacggac tttgtcgcca cgatgctgca
     2401 caccgaagcc gaatatgcct acggattgac gcttccctcc tttgccgcca tgtttaccag
     2461 gctctacatg caacgctatg ggctgacgga gcgtgagctg gccatggtcg ccgtcaagga
     2521 ccatgaaaac ggatccaaaa atccctatgc ccacgtcaag gtaccctgca cgcttgaggc
     2581 gatccacgat ggcgagaacg ccaacgtcgt caacaacatc gtcgctgatc cactccgtct
     2641 gtatggaatg tgtccggtct ccgatggagc tgcctccctt ctcctttgtg cgactgattc
     2701 tccaaagatg aagtttttca aaaaacagcc catccgaatc agcggggtcg ggtctgccac
     2761 cgatacccag tgcgttcact tacgcaagga tccgctcgtt ctggatgcag tccgcctggc
     2821 cgccgaacgg gcctatgcca tggccggtct cggacccaag gatatctcct ttgccgaact
     2881 gcatgatgcc tttgtgattc tcgaattggc catctccgaa gaagtcggat ttttcgaacg
     2941 cggcaaatcc cgtgaagcgg ttgtcaaggg cgagacacgg atcgacggaa gactccccat
     3001 caatacctcg ggcggactca aggccaaggg ccatccgctg ggcgcgaccg gagtctccca
     3061 gattttcgag ctcgtcaaac agcttcgcgg tgaagccgaa gaaggccgtc gggtcaccaa
     3121 tccgaaacac ggtttggccg tcaactttgg cggatttgga aacaatgtcg ttgcaacgat
     3181 ctgttccagg gaggacgtat gaacttcaat ttcaacaagt acggcccaaa aaaacacgag
     3241 gtctgtgcct acaagtgtcc gggttgcgga aaactctact atccggcggt catggtctgc
     3301 gaccagtgcc atacacgccg ggatccatcc aatttttatt tcaaggactg ggaaaaggta
     3361 ccccttgaag gtgactgcac cctcctgacc tggaccaggg tctatgcact ccccgagggg
     3421 tatatgaaaa aataccagga tttcggaatc gtcgagtttg ccaacggact ccgggccagc
     3481 ggccagcttg atgttgccac gccggagatc gggatgaagc taaaaagctc ggtcggagtt
     3541 gtcaaagaac gcgtcggaaa agacaattac gggtttatct ttatcagccc ctgagcgaat
     3601 caccttg
//
LOCUS       DAOSHE010000204         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24041_length_3589_cov_3.094793, whole genome shotgun sequence.
ACCESSION   DAOSHE010000204 DAOSHE010000000
VERSION     DAOSHE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24041_length_3589_cov_3.094793"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..526
                     /gene="ribE"
                     /locus_tag="PLD82_06445"
     CDS             53..526
                     /gene="ribE"
                     /locus_tag="PLD82_06445"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOG65060.1"
                     /translation="MKSINGSLDAQGKRFALVVGRFNSFISERLIEGALDCIVRHGGI
                     EDAVTVIRVPGSFEITAAVRAAVDSGRYDAVITLGALIRGSTPHFDYVARSVTTGVSA
                     IAAEAKTVVTFGVLTTDTIEQAIERAGTKSGNKGFDAAMAAIEMVNLLAAVRNGG"
     gene            532..954
                     /gene="nusB"
                     /locus_tag="PLD82_06450"
     CDS             532..954
                     /gene="nusB"
                     /locus_tag="PLD82_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG65061.1"
                     /translation="MGVREKARDIAVQALYQLSLRGMEDLEGIAAFEWIDEDSYEGIT
                     SQAGTWFRGAAAALASIDAIIDRHLENWAPDRVGRMERAILRLGTYELTGSEQIAPEI
                     VINECVNLAKRYCDFDSYKFVNGVLDAVAGSLGRKEIS"
     gene            914..>2464
                     /locus_tag="PLD82_06455"
     CDS             914..>2464
                     /locus_tag="PLD82_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65062.1"
                     /translation="MRLPDLSVGKKYLERVAAGVLTRIESRTGMRRNQILVAALLLAL
                     MLTSLLLFFSLKNRPHDPGGFGVPDDFGVLAAETVVHPQSPLAPAVGAWRRGSPWVAL
                     DLLRQAQTNHLTTEEHLAALNYAGHIRIWIGDLAAAGAAFQAGAGLKKNAGSAYGLGR
                     VAELKNDYPLAVRQYRTAVEYRPDFALAWRRMGDVLMRQKDFEQAVLAYTRSLEISSL
                     DITRYRLGEALVSQGQGEAARQHFQYLLEHAHHSLLVGYAAARLGDLEAVRGDLKRAA
                     ELYQQAVRVCPETAAFRHNLGGILVRAGRLEEAMSVYLALRGAILKKKNPTISDTELR
                     QSLARVIGETWYDRNDTAAALRFLSEAGREDSEVLAMLGDLFFLRGEYDDALGYYARV
                     VANLPGQRLGLVALVNTGTIWLERMKPERALHAWEKALQIEPENPRVLCNIGLAHLAL
                     RDNKAAETAFRKAWTLDQSLTNALALLAATMPVGEIITLLREGEDKASGWFALHTLGR
                     LYHRKREYG"
     assembly_gap    2465..2474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2475..2935
                     /locus_tag="PLD82_06460"
     CDS             <2475..2935
                     /locus_tag="PLD82_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65063.1"
                     /translation="VLERAIKAAARPSEADASRLLLARCRMARDEHDLAARELQPIGE
                     RYAQDPLWLYQMGLLSWKTGSLTNARRYFSSAEQAQASPRLAAAIRYWLGNMEWKEGH
                     IAGALAGYRAALTLHPGHTAAAVNAAWCVNRLAEGRKQGKRSRLDEGTLR"
     gene            2916..>3589
                     /locus_tag="PLD82_06465"
     CDS             2916..>3589
                     /locus_tag="PLD82_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG65064.1"
                     /translation="MKEPCADLHTHSTCSDGTQTPEEVVAAADFSGVAWLALTDHDTI
                     DGVPRVRAAIAARELPIELITGAELSCHSETDGEVHILAYGFDEEAPSMLEHMRDFRD
                     QRHIRLGRMLDLLRDLGCPLEKEDVMAFCKGESVSRVHVASALARHGYVANPSEAFRL
                     YLSNGGPAYVPRKKYSPEQMIPMIHEWGGVAVMAHPNYFDSVEADDAGIESRLERYIA
                     MGIDGF"
BASE COUNT          668 a         1016 c         1148 g          747 t
ORIGIN      
        1 caagggtgcg accatcatca ctatgccctg acgggttccg ctggaggaag atatgaaatc
       61 gatcaatggt tcgctggatg cacagggcaa gcggtttgcc ctggttgtcg gccgtttcaa
      121 cagtttcatc tccgagcgct tgattgaggg tgccctggac tgtattgtcc gtcacggggg
      181 gatcgaggac gccgtcacgg tcatccgggt tccgggttcc ttcgagatca ccgcggccgt
      241 ccgggctgcc gtcgactccg gacgatacga cgcggtgatt accctcgggg ccctgatccg
      301 cggatcgacc ccccattttg attacgtggc ccgttccgtg acgacagggg tttcggccat
      361 cgccgccgag gcgaagacgg ttgtcacctt tggcgtcctc accacggata cgatcgagca
      421 ggccatcgaa cgggccggaa ccaagagcgg caacaaaggc tttgatgccg cgatggcggc
      481 catcgagatg gtcaatcttc tggccgcagt ccggaacggt ggctgacagg gatgggcgtc
      541 agggaaaaag ccagggatat cgccgtgcag gctctctatc agctctcgct gcgggggatg
      601 gaagatctcg aagggattgc cgccttcgaa tggattgatg aagattccta cgagggcatt
      661 acgtcacagg cagggacctg gttccggggg gctgccgcgg cgctggcttc cattgatgcc
      721 atcattgacc ggcatctcga gaactgggct ccggaccgcg tcggccggat ggagcgggca
      781 atccttcgtc tggggacata tgaattgacg gggagcgagc agatcgctcc ggagattgtg
      841 atcaacgaat gtgtcaatct cgccaagcgg tattgcgatt ttgattccta caaatttgtc
      901 aacggggtac tggatgcggt tgccggatct ctcggtcgga aagaaatatc ttgagcgggt
      961 tgcagccgga gtattgaccc gaatcgagtc ccggaccggg atgcgacgga accagattct
     1021 ggtggccgcc cttctcctgg ccctgatgtt gacatccctt ctgctgtttt tttccctcaa
     1081 aaacaggcca catgatccgg gcgggttcgg tgttccggac gatttcggtg ttctggctgc
     1141 cgagacggtt gtccatcctc aatcccccct ggctcctgca gtcggggcct ggagacgggg
     1201 ttctccctgg gtggcccttg acctcctcag gcaggcccag acaaaccacc tgacaaccga
     1261 agaacacctg gctgccctca actatgccgg acatatccgg atctggatcg gggacctcgc
     1321 ggctgcgggg gcagcgttcc aggctggcgc cggtttgaaa aaaaatgccg ggtctgccta
     1381 cggattgggc agggtggccg aactcaaaaa tgattatccc ctggccgtca ggcagtatcg
     1441 gactgccgtt gaataccggc cggactttgc cctggcctgg cgacgcatgg gtgatgtcct
     1501 gatgcggcaa aaggattttg aacaagcggt gctggcctat actcggtccc tggagatctc
     1561 gtccctcgac ataacccgct atcgtttggg tgaggccttg gtctcgcagg ggcagggtga
     1621 ggccgcccgg cagcatttcc agtacctcct ggagcacgcc catcactccc tcctcgtggg
     1681 atatgcggcc gccaggctcg gtgatctcga ggccgtccgc ggtgatctca agcgtgcggc
     1741 ggagttgtac cagcaggccg tacgtgtctg tccggaaacc gcggcttttc gccataatct
     1801 gggcgggatt cttgtccgtg ccggcaggct tgaggaggcc atgtccgtct atctggcttt
     1861 gaggggggcg atcctcaaaa agaagaaccc gacaatctcc gatacggaac tccggcaatc
     1921 gctggcccgc gtcatcggag agacctggta cgaccggaat gataccgcgg cggccctgcg
     1981 gtttctgtcc gaagcggggc gggaggattc ggaggtcctg gccatgctcg gggatctttt
     2041 tttccttcgt ggagagtatg atgatgcgct cgggtattac gcccgggtcg ttgccaacct
     2101 gccggggcag agactcggtc tggtggccct tgtcaataca ggaacgatct ggctcgagcg
     2161 gatgaagccc gagcgggcac tgcatgcctg ggaaaaagct ctccagattg aacctgagaa
     2221 tccccgggtc ctctgcaata tcggtctggc ccacctggcg ctccgtgaca acaaagcggc
     2281 ggagaccgct tttcgcaagg cctggacgct ggatcagagt ttgacgaatg ccctggccct
     2341 gctggccgca accatgccgg tcggcgagat aatcaccctg ctcagggagg gggaggacaa
     2401 agcatccggc tggtttgccc tgcatacgct cggccgtctc taccaccgga agcgggaata
     2461 cgggnnnnnn nnnnaggttc tggaacgggc catcaaggcg gctgcccggc catccgaagc
     2521 ggatgccagc cggcttttgt tggcgcgttg ccggatggct cgggacgagc acgatttggc
     2581 tgcccgggaa ctccagccga tcggcgaacg gtatgcccag gatcccctct ggctctacca
     2641 gatgggactg ttgagctgga agaccgggtc gttgaccaat gcccggcggt atttttcttc
     2701 tgctgaacag gcgcaagcct caccgcgcct tgcggcggcc atacggtatt ggctgggtaa
     2761 tatggagtgg aaggaaggcc atatcgccgg agcactggcc gggtatcggg ctgcgctgac
     2821 actccaccca gggcacacgg cagcggccgt caatgcggcc tggtgcgtca accgtctggc
     2881 tgaggggcgg aaacaaggca agaggagtcg attggatgaa ggaaccctgc gctgatttgc
     2941 acacgcacag cacctgttcg gatggcacac agacacccga ggaagtcgtg gccgccgccg
     3001 atttctcggg agtagcctgg ctggctttga ccgatcatga tacgatcgac ggggttcccc
     3061 gggtacgcgc cgcgattgcc gcccgtgaat tgccgattga gctgattacc ggtgctgaac
     3121 tctcttgcca cagtgaaacg gatggagagg tgcatattct cgcatacgga tttgacgaag
     3181 aggctccttc catgctcgag catatgcggg atttccgcga tcaacggcat atccggctcg
     3241 gccggatgtt ggacctcctg cgggatctgg gttgccccct ggaaaaggaa gacgtcatgg
     3301 cattttgcaa gggcgagagt gtttcccgtg tccatgttgc ttctgctttg gcgcggcacg
     3361 gctatgttgc caatccttcc gaagcgttcc ggctctatct ctccaacggc gggccggcct
     3421 acgttccgcg gaaaaaatac tcgcccgagc agatgatccc catgatccac gagtggggcg
     3481 gggtcgccgt catggcgcat cccaattatt tcgattcagt cgaggccgat gatgccggta
     3541 tcgagtcccg tctggaacgc tatattgcga tggggattga cgggttcga
//
LOCUS       DAOSHE010000205         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24086_length_3585_cov_4.186119, whole genome shotgun sequence.
ACCESSION   DAOSHE010000205 DAOSHE010000000
VERSION     DAOSHE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24086_length_3585_cov_4.186119"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLD82_06470"
     CDS             <1..663
                     /locus_tag="PLD82_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG65065.1"
                     /translation="KEERERNVLIAIDISASQFFGSHDKTKKMLSAELAAVFSLSALS
                     SKDKIGCLLFSDRIEEMVMPKRGRTHVLRIIRDVLGHQPKGRGTDIALALDHINRILP
                     RKTIVILISDFYGEGYEKAMQRTALRHDLVPIVLRDPAELAMTGNGLFVVEDAETGKI
                     EPCWMGSKKLGREFAETSSLREAGLTRFFRKQQIDFLTVRSGDAYSEALMEFFRRRVA
                     RQ"
     gene            660..1583
                     /locus_tag="PLD82_06475"
     CDS             660..1583
                     /locus_tag="PLD82_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65066.1"
                     /translation="MTRRIVSALLFSLLPISSLLATGVTVEWATSRVETGSHVGFVVH
                     LPKNAVLDQVSVSIRQTNADWELAGEKESALTVSGKSITGRIQCFAVEDGMFPGLRIG
                     WVENGRKQFFSSPPVWFFVAAPVTDLSAQPPVRGLRGPVSAGLPWLLIFGIFLVLVLL
                     GWTWFIVRKRKKGFVLPDANQIADPWELASRRIEILRENLPEDELAIKEHIFLLTETL
                     KQYLSGRSGLHLVEQTSREVVASVREFAWAGNDMTDEIREWLERGDMAKFARQIPTPG
                     ELTGYLESLAAWLERIESQWKTMEQAREGEP"
     gene            1580..2626
                     /locus_tag="PLD82_06480"
     CDS             1580..2626
                     /locus_tag="PLD82_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG65067.1"
                     /translation="MNFRDPLLLLLLLPGVVALSLLMIKTRRTLPVVRWSALVVIPES
                     VRRAGRWRLLMVQGFVILSWILAVIGFAGPQIIHSYEKRYSEGIDIMIALDVSGSMRS
                     VDDPAAIRASHSQGYYHDTAGILTNRLQHARRLTADFLRKRMDDRLGLTVFAGYAITK
                     CPLTFDHELLQRILSEVDFTDVQQQSTAIGMAMANSLQRLVSSKAKSRVLILVTDGVN
                     NAGTIDPETATEMAKALGVKVYTVGVGSDEPLLPTQKPDYYVRSQTVIDEDSLKGIAE
                     KTGGAYFRAADARMLENIYARIDSLEKSMIERKYFFEREEFYQPFILAALASLVISLI
                     LRHCVFRILPGGAV"
     gene            2623..>3585
                     /locus_tag="PLD82_06485"
     CDS             2623..>3585
                     /locus_tag="PLD82_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG65068.1"
                     /translation="MIFQNPEWHYLLLLIPASVILYLLGRMHWKRLCTRLKGGSALVR
                     QIDASPARRYLRFALVLGAIFFFVVALMRPRWGARDGGETELGIDIAIVLDISRSMSV
                     RDVMPSRLERTVAELSALLKELDGNRFALVLFAGAAYIQCPLTADAGAIREFLENASP
                     ALIVLQGTNIEDGLEKASRALKSQFKRNRIVLLVSDGENHEGDAQASARRLYRDQSIR
                     LFTLGVGTSGGAGLASEDGDGGDIVSKLDEETLKSVAKEGGGEYYRIGNERFDTARLA
                     SSINSIEKSGVLMQRPDTLVDRYPLFLGAGRCIKTPLFSIEFMLE"
BASE COUNT          778 a          938 c         1015 g          854 t
ORIGIN      
        1 aaggaagagc gcgagcggaa cgtcctgatt gccatcgata tcagtgcgtc ccagtttttc
       61 ggaagtcatg acaagacaaa aaaaatgctc tcggcggaac tggctgccgt tttctccctc
      121 tccgcgcttt ccagcaagga caagatcggg tgtcttcttt tcagtgaccg aatcgaggag
      181 atggtcatgc cgaagcgggg acgcacccat gttttgcgaa tcatccggga tgtcctcggt
      241 catcaaccca agggtagggg gacggatatc gctctggccc tcgatcacat caacagaatc
      301 cttccacgaa agaccattgt catcctcatc tcggattttt acggtgaggg gtatgaaaaa
      361 gccatgcagc gcacagccct gcggcatgat cttgttccga ttgttctccg tgacccggcc
      421 gagcttgcca tgaccgggaa cgggctcttt gtcgtcgagg atgccgagac aggaaaaatt
      481 gaaccttgct ggatggggag caaaaaactt ggacgggaat tcgccgaaac atcatccctc
      541 cgggaggccg gactcaccag gtttttcagg aaacagcaga tcgatttttt gaccgtgcgt
      601 tccggggatg cctacagcga agcattgatg gagtttttca gaaggagggt tgccagacaa
      661 tgacgcgtcg catcgtctcc gctctcctct tttctcttct tccaatctcg tcacttttgg
      721 caaccggtgt cacagtggaa tgggcgacat ccagggtcga gacaggttcc catgtcggtt
      781 tcgttgttca tcttccaaag aatgctgtcc ttgatcaggt ctccgtttcc atcaggcaga
      841 cgaacgccga ctgggagctt gccggtgaaa aagaatccgc tttgacggtt tcaggaaaat
      901 cgatcaccgg gcggatccag tgttttgccg tggaagatgg catgtttccg ggactgagaa
      961 tcggctgggt ggaaaacgga aggaaacagt ttttttccag tccccccgtc tggttctttg
     1021 ttgcagctcc tgtcacggat ctctcggctc aaccgccggt tcgcggactg aggggaccgg
     1081 tcagtgcggg cctgccctgg cttctgatct tcgggatatt tctcgtcctg gtcctcctgg
     1141 gatggacttg gttcatcgta cgtaaaagga aaaagggatt tgttcttccc gatgcaaacc
     1201 agattgccga tccctgggaa ttggcctcca ggaggatcga gattctcaga gaaaatcttc
     1261 ccgaggatga acttgccatc aaggagcaca tcttcctcct gaccgagacc ctgaagcagt
     1321 acctcagtgg ccgcagcggt ttgcatcttg tcgaacagac cagccgggag gttgttgctt
     1381 ctgtcaggga atttgcctgg gcaggaaatg acatgacgga tgaaatcagg gaatggcttg
     1441 aacgcggaga tatggcaaag tttgcacggc agatacccac gcccggtgag ttgaccgggt
     1501 atctcgaaag tcttgctgcc tggctggaac gtatcgagtc ccagtggaaa accatggagc
     1561 aggcacggga gggcgaacca tgaattttcg tgatcccctt ctgctcttgc ttctgctccc
     1621 cggtgttgtt gccctttcgt tgctcatgat caaaacacgt cggactctcc cggttgtacg
     1681 ctggagtgcc cttgtggtca ttcccgaaag tgtacgcagg gcaggacgct ggcgattgct
     1741 catggtgcag gggtttgtca tcctgtcatg gatcctcgct gtgatcgggt ttgccggtcc
     1801 acagatcatc cacagctacg agaagagata cagcgaaggt atcgacatca tgatcgccct
     1861 ggatgtttcg ggcagtatgc gctcggtgga tgatccggcg gcaatccgcg catcccacag
     1921 ccaagggtat tatcatgata cagccggtat cctcacaaac agattgcagc atgcgcggcg
     1981 actgaccgcg gatttcctca ggaagagaat ggatgacagg cttggtctca cagtgtttgc
     2041 cgggtatgcc atcacgaagt gtccgctcac gttcgatcat gaactgctcc agaggattct
     2101 gtcggaagtc gactttacgg acgtccaaca gcaatcgacg gccatcggca tggccatggc
     2161 taattcactc cagcgtctcg tttcgtccaa agcgaagagc cgggtcctga tcctggtcac
     2221 ggacggggtc aacaatgccg gtacaatcga tcccgaaacc gcgacagaga tggctaaggc
     2281 cctcggtgtc aaggtctaca cagtcggtgt cgggagtgat gaacccctct tgccgacaca
     2341 aaaacccgac tactatgtcc ggagccagac tgtgatcgac gaagattccc tcaaggggat
     2401 cgccgaaaaa acaggtggag cgtatttcag ggcagccgat gccagaatgc tcgagaatat
     2461 ctatgcccgg atcgattcgc tcgaaaagtc aatgatcgag cggaaatact ttttcgagcg
     2521 tgaggagttt tatcaaccgt tcattctggc ggctctggca tcccttgtca tctccctgat
     2581 tctgcggcat tgtgtgttca ggatcctccc cggaggtgct gtatgatatt ccagaatccg
     2641 gaatggcact accttttgtt gctgattccc gcatccgtga tcctctatct tctgggacgg
     2701 atgcattgga agagactctg taccaggctg aaaggtggct cggctcttgt gcggcagatc
     2761 gatgcctctc cggcccgccg ctatctccgt ttcgcactcg tcctcggggc catcttcttt
     2821 ttcgtagtcg ccttgatgcg gccccgctgg ggtgcacggg atggcggaga gacggaactc
     2881 ggcatcgata ttgccattgt tctcgacatc tcgaggagca tgagcgtccg tgatgtcatg
     2941 ccgagcaggc ttgaacggac ggtcgccgaa ctctcggcac tcctcaagga acttgacggg
     3001 aacaggtttg cgctcgtctt gtttgccgga gctgcctata tccagtgtcc cctgaccgca
     3061 gatgccggtg cgatccgtga atttctcgaa aacgcatcac ccgccctgat cgtgctccag
     3121 ggaaccaata tcgaagacgg actggaaaag gcctcccggg cactcaagag ccagttcaag
     3181 cgcaacagga tagtccttct tgtcagtgat ggtgaaaacc acgaagggga tgctcaggct
     3241 tcggcccgga gactgtaccg ggatcaatca atccggttgt ttaccctggg tgttggcaca
     3301 tccggaggtg caggccttgc ttctgaagat ggcgatggtg gagatattgt ttcgaagctt
     3361 gatgaagaga cgttgaagtc tgtggccaag gaaggtggtg gcgagtatta ccggatcgga
     3421 aacgaacggt ttgatactgc ccgactggcc tctagcataa actctataga aaagagcggc
     3481 gtcttgatgc aacgtcccga tacacttgtg gaccgctatc ccttgtttct cggtgcggga
     3541 cgttgcatca agacgccgct cttttctata gagtttatgc tagag
//
LOCUS       DAOSHE010000206         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24185_length_3574_cov_3.892583, whole genome shotgun sequence.
ACCESSION   DAOSHE010000206 DAOSHE010000000
VERSION     DAOSHE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24185_length_3574_cov_3.892583"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLD82_06490"
     CDS             complement(<1..861)
                     /locus_tag="PLD82_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOG65069.1"
                     /translation="MAIMILFASIMLIIALPGLRRMKSVEGYVTGNRESGGLLVGASL
                     VATIIGASSTLGMADLARTFGGAAFWWLGLGAAGLLLMAWLTVRPLWNLKAISIADAV
                     SRLAGRGAGRLTGVVIVLAWTGIIAAQLVAVGSVLSMAAEADPAVVILITGLAVTLYT
                     TIGGQRSVIRTDLVQLVILVVGVLAVFLMLAFPGSEADSGLVLQPLLHAGFDLEKLVF
                     MAVPLLGAYAAGPDMFSRINSARSVKAARRGLVWAACVVVCFGALITSLALISLQISD
                     SGGKKPLLGAV"
     gene            923..1714
                     /locus_tag="PLD82_06495"
     CDS             923..1714
                     /locus_tag="PLD82_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65070.1"
                     /translation="MTESITHNDESRRMFLENACAIMLHLDTSGNLVWVNDWTLEVFG
                     FNREELVGHPIVGTLVPYTESTGRDLEAMFRRFYEFPMDFITNINENTKKNGERIWVA
                     WTNRALFDSEGWINGFLSVGVEITNEQHDLLRLRNENTLLRSTLNAFDDAIFITDGNG
                     QPKGYNKPLMELFPSTVEAFASRNVMQLLEIIAGEVRLEDREVFRQTAGTALASAGDM
                     GPGDFVQGKIKLIHGRQGANIAWQARPRTINGVFEGLLWIFRNSG"
     gene            complement(1788..3155)
                     /locus_tag="PLD82_06500"
     CDS             complement(1788..3155)
                     /locus_tag="PLD82_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65071.1"
                     /translation="MNNARGVFFRFITMMLLLLVVLLAGTGILLISLQEISLYREKEQ
                     HADQMTSFVATISALYIERFNYSALHQIAEKLQPKTNASPGNGEDDTGILSVVIRNTK
                     HGAKTAGDVLHFNGVNQDKITVDRSLWLVREFPCIPTGKPKESQLGTVTIIFSLEEIN
                     RTVVTMRLVFSLVMVGSILILGVFAILLLRKLVGRPLFEIAGQLDQASDQISQASMQI
                     ADGSSALARGANEQAERLRDTDESIGDLRTMGEEMRQLADEASNLMNVNLENSGQALR
                     NLTALNAQMGSIRNDSGKMAKIIKAIDEIAFQTNLLAINAAIEAARAGTAGEGFAVVA
                     DEVRILAQDTAQAAQDTSGLLEGTIDQVASAAASIDGMNREFEGIVESATMIGEKTAR
                     VAEASAQSAERIVRVFDASKRIEEVTQNVAASSEESAATAQELAAQSRMLANLVGTLR
                     RLLGR"
     gene            complement(3162..>3574)
                     /locus_tag="PLD82_06505"
     CDS             complement(3162..>3574)
                     /locus_tag="PLD82_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG65072.1"
                     /translation="NNPARGHDLALELYGQGAEVVYAVAGGTGNGIIQAAKEKNRLVL
                     GVDSNQDHLAPGYVLTSMMKRADVAVTNIIGRYLAGTLEGRRVYRFDLKNHGVSLTDF
                     EYTEDRVSAEMRRELDEASKKIADGRLAVPTVLR"
BASE COUNT          798 a         1124 c          950 g          702 t
ORIGIN      
        1 aacggcaccg aggaggggct tcttaccgcc cgaatcggaa atctggagcg agatcagtgc
       61 cagactggta atcaacgcgc cgaaacagac caccacacat gccgcccaga caagcccgcg
      121 gcgggccgct ttgactgatc gggcggaatt gatccgcgaa aacatgtccg gtccggctgc
      181 ataggccccc aggaggggga ccgccatgaa gaccagtttt tcaagatcga agccagcgtg
      241 caaaaggggc tggagcacga gaccggaatc ggcctcactt ccgggaaagg ccagcatgag
      301 aaaaacggcc agtacgccta caacaagaat cacaagctgg acaaggtctg tccggatcac
      361 actgcgctgt cctccgatcg tcgtgtagag cgtcacagcc agcccggtaa tcaggatcac
      421 caccgcaggg tccgcctccg ctgccatcga gagaacactc cccacggcca cgagttgcgc
      481 cgcgatgatc ccggtccagg caaggacgat gaccacccct gtcaatcttc cggccccccg
      541 acccgcgagc cggctgacgg catccgcaat cgagatggcc ttgagattcc agagaggccg
      601 gacggtgagc caggccatca ggaggaggcc ggccgcaccc aggccgagcc accaaaaagc
      661 ggcacccccg aaggtccgcg ccagatcggc cattccgagc gtcgaagacg ccccgataat
      721 cgttgccacc aaggacgcac cgacgagaag accgccggac tccctgttcc ccgtcacata
      781 gccttcgaca ctcttcatcc tcctcagccc gggcagggca ataatgagca tgatcgaggc
      841 gaagagaatc atgattgcca tacgcatccc ctctcgcata tactggaacc ggccggaaac
      901 ccgggagtac gaggtacaac caatgactga gtccatcaca cacaacgacg aatcccgccg
      961 gatgttcctc gagaacgcct gtgccatcat gctccatctg gacacttccg gaaacctggt
     1021 ctgggtcaac gactggaccc tggaggtttt cggattcaac cgggaagaac tcgtcggaca
     1081 tcccatcgtc ggcaccctgg tcccgtatac cgaatcaacc ggccgggatc ttgaggccat
     1141 gttccgccgg ttttacgaat tcccgatgga cttcatcaca aacatcaacg aaaatacgaa
     1201 aaagaacggc gaacggatat gggtggcctg gaccaaccgg gccctctttg acagcgaggg
     1261 ctggatcaac ggttttctct cggtcggtgt cgagatcaca aacgagcaac acgacctctt
     1321 gcgcttgcgc aacgaaaaca ccctcctccg cagtaccctg aatgcctttg atgacgcgat
     1381 cttcatcacg gacggcaacg ggcagcccaa gggatacaac aagcctttga tggaactctt
     1441 cccgtccacc gtcgaagcct ttgcttcccg gaatgtgatg cagctcctcg agatcatcgc
     1501 cggagaagtc cggttggagg accgggaagt cttccgtcaa accgcaggaa cggccctcgc
     1561 gtcggccggg gatatgggac caggagattt tgtccaagga aagatcaagc tcatccatgg
     1621 caggcagggg gccaatatcg cctggcaggc ccgacctcgc accatcaacg gtgtttttga
     1681 aggactgctc tggatcttcc gcaactcggg ttgagacaag tctcttttgc tgaacacagt
     1741 acccccggaa agggctgcat gtgggcgggg agacttcgtc ccgcagatca ccgacccaac
     1801 agtctccgca acgttccgac cagattggcc agcatccggg actgggccgc gagttcctgt
     1861 gcggttgccg cagattcctc cgaactcgcg gcaacgttct gtgtgacctc ttcaatccgt
     1921 tttgaagcat caaagacccg cacaatcctt tcagctgact gggcactggc ctcggccacc
     1981 cgggcggtct tctctccgat cattgtcgcg gattcgacga taccttcgaa ttcccggttc
     2041 attccgtcaa ttgatgcggc cgccgaggca acctgatcga tcgtcccctc gagcagaccg
     2101 ctcgtatcct gggcagcctg ggcggtatcc tgtgcaagaa tccggacctc gtccgccaca
     2161 acggcaaaac cctctcccgc tgtaccggcc cgggcggctt cgatggccgc attgatcgcc
     2221 agaaggtttg tctggaaggc aatctcgtca atcgccttga tgatcttggc catcttcccg
     2281 ctgtcgttgc ggatacttcc catctgggcg ttcaaggccg tcagattccg caaagcctgc
     2341 ccggaattct cgaggttgac gttcatcagg ttcgatgctt catccgcaag ctgacgcatc
     2401 tcctcaccca tcgtccgcag atcgccgatg gattcgtctg tatccctgag gcgttccgct
     2461 tgctcattcg ctccccgcgc cagcgcggaa ctcccgtcgg caatctgcat cgaggcctgg
     2521 ctgatctggt cggaagcctg atcaagctgc cccgcgatct cgaagagggg ccgcccgacg
     2581 agtttccgga ggaggagaat ggcgaacaca cccagaatga ggatgctccc gaccatcacg
     2641 agggagaaca ccagacgcat cgtcacaacc gtcctgttga tttcctccag ggagaaaatg
     2701 atcgtcacgg tgcccagttg actctctttg ggttttcccg tcggaataca ggggaattcc
     2761 ctgaccagcc agagactgcg gtcaacagtg attttatcct ggttgacccc gttgaaatgg
     2821 aggacatcac ctgcagtctt cgcaccgtgc ttggtgtttc ggatcacgac cgaaagaatc
     2881 cctgtatcat cctctccgtt tccgggcgag gcattcgtct ttggctggag tttttcagca
     2941 atctgatgaa gggctgaata gttgaatcgc tcaatataga gggcactgat cgtcgcgaca
     3001 aacgaagtca tctggtccgc atgctgctct ttttcccggt acagggagat ctcctgcaga
     3061 ctgatcaaaa gaatccccgt gccggccaga agaacgacaa ggagaagcag catcatcgtg
     3121 ataaaacgga aaaaaacccc gcgggcattg ttcatggttt tctacctcaa cactgtcgga
     3181 acagccagcc tgccatcggc aatctttttc gatgcctcat ccaattccct gcgcatctct
     3241 gccgaaaccc tgtcctctgt atattcgaaa tcggtcaaac tgacaccatg attcttcaaa
     3301 tcgaaccggt agacgcgccg accctcaagt gtccctgcca gatagcgacc gatgatattg
     3361 gtcactgcaa cgtccgcccg cttcatcatg ctcgtcagga cataccccgg ggcaagatga
     3421 tcctgattgg agtctacccc gagaaccaag cggttcttct ccttggccgc ctggatgatc
     3481 ccgtttcccg tgcccccggc gacagcatag acgacctcgg ctccctgtcc atacagttcg
     3541 agcgcgagat catggccccg cgccggattg ttga
//
LOCUS       DAOSHE010000207         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24228_length_3570_cov_3.247511, whole genome shotgun sequence.
ACCESSION   DAOSHE010000207 DAOSHE010000000
VERSION     DAOSHE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24228_length_3570_cov_3.247511"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLD82_06510"
     CDS             complement(<1..161)
                     /locus_tag="PLD82_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG65073.1"
                     /translation="MAARMTDRTRIRNFSIIAHIDHGKSTLADRMLEETGILASGKHD
                     AQVLDNMEL"
     gene            complement(352..1149)
                     /locus_tag="PLD82_06515"
     CDS             complement(352..1149)
                     /locus_tag="PLD82_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOG65074.1"
                     /translation="MDFPVFLVTAFIAALVSAFGSGLLSTFVVLRRASFTSEAFAHIA
                     FAGMAFALLAGLPYGLVTFAFVAAVAFTVGLASSKMAVAEVNLTTIFLAVSMATGVLL
                     LSLNKGYTPDIADYLFGNILLVGEDDLFVLSILAVVDLVWLAVFFRAMYYTAYNETAA
                     AVYRIPVRLVTTGFLVLLACNIVVAVKIAGVVMITAGLVLPGTIALLITRRILMAHAI
                     AVLVSLGATIGGFLLSYTGDLPTGPLVVLTQFGAFILVLAVRGLVRR"
     gene            complement(1159..1887)
                     /locus_tag="PLD82_06520"
     CDS             complement(1159..1887)
                     /locus_tag="PLD82_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOG65075.1"
                     /translation="MIEVRSVGLKIGTSTVLENVSFTLSDGDFTAILGPNGAGKSTLI
                     KILLGLVPEHTGQVLYDGVPAREWLRKNHVGYLPQREMVPRLFPGTAADIVRLGLLAC
                     RGAFARLPANAAQQVHEALEMTGAAHLEHRAIDALSGGELQRVLLARAIVGGSRYLFL
                     DEPEAGVDSQQIAGFYSLLSKLNADGRTVLLVSHDINVILKTVSSVLCLNRTLHCHTS
                     ADLVSAEVVQKTWGDVLRLVDKHF"
     gene            complement(1884..2786)
                     /locus_tag="PLD82_06525"
     CDS             complement(1884..2786)
                     /locus_tag="PLD82_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOG65076.1"
                     /translation="MRKLVWIVTLVWFGVLACGRSGDGIDKRLVLATIRPYALIAAEI
                     AGARLRVETLLPVSASPHTWSPRPSDVQRISRAGLIIANGLGLEEKLGRFFHDQSART
                     ILAADILGTPGREEKHAEGHEDDGHGHGRNTDPHLWTDPVLMVRFAGVLAKRFGQHDP
                     EGRDLFANNLISFSNRVMALDARIRDEAAGYARRAVVTFHDAFPRLFARYGITRVAAL
                     MATPGKEPSAAELAGLGRKIRDAGVRALYREPQMDPRSVNILAKEYGLPVYSVDPIGQ
                     DPGVTNYAALIRYNWEMFAKGFAR"
     gene            complement(3101..>3570)
                     /locus_tag="PLD82_06530"
     CDS             complement(3101..>3570)
                     /locus_tag="PLD82_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOG65077.1"
                     /translation="IEDWPERLEPAGREILKVLGRSLKLRSVSAGGCNGCELELNASH
                     NVNFDLNRYGVEVVASPRHADALFLTGPVSRNMAPAVRDVWEMMPEPKLLIVAGACAI
                     SGGVFADSPDLDRTFLQEHVPDLYIPGCPAHPLTIVNGILGLLGRKKAGLFSL"
BASE COUNT          852 a         1105 c          977 g          636 t
ORIGIN      
        1 tcgagctcca tgttgtcgag cacctgggca tcatgcttgc cggatgcaag gatcccggtc
       61 tcctccagca tacggtcagc cagtgtggat tttccgtgat caatatgggc tataatagaa
      121 aaattgcgta tgcgtgtgcg gtccgtcatg cgggcagcca tccattctgg ttcgttcttg
      181 gggaacagtc cttgtatcgt tacagggaaa gatactactc gccggggaaa aggagagaag
      241 ggtttgaacc ccctctccgt gaacactcca ccggccgaac agaaacacgg gtattaggaa
      301 agtcgggatt tcaatactgg tcagggcgga gggatttcaa acctctcgct actacctgcg
      361 caccagcccc cgcacagcga gcaccaatat gaaggctccg aactgggtca ggacaaccag
      421 gggcccggta ggcagatccc cggtgtagga caacaggaag ccgccgatgg tcgctccaag
      481 ggagaccaga acggcaatcg catgcgccat cagtatccgt cgtgtaatga ggagggcaat
      541 cgtaccgggg agaacaaggc ccgcggtgat cattaccacc ccggcaatct tgacagccac
      601 aacaatattg cacgccagga gaacgagaaa gccggtcgtc accagccgaa cggggattcg
      661 atagacggcg gctgccgtct cgttataggc cgtgtaatac atcgcccgga aaaacacggc
      721 cagccagacc agatccacca cggccagaat cgacaggaca aaaagatcgt cctccccgac
      781 cagaagtatg tttccaaaga ggtagtctgc aatatccgga gtatatccct tgttgaggga
      841 gagcaggaga acgccggttg ccatggaaac agcaagaaaa atcgttgtca gattgacttc
      901 agcgaccgcc atttttgacg acgcgagtcc gacggtaaaa gccactgctg cgacaaaggc
      961 aaaggtcaca agcccgtagg gtaatcccgc caacagggcg aaggccattc cggcaaaagc
     1021 aatgtgggca aaggcttcag aggtaaagga ggcccgacgc aacaccacaa aagtcgagag
     1081 cagaccgctc ccgaacgcgg aaaccaaggc ggcaatgaat gctgtcacaa gaaaaaccgg
     1141 aaaatccaca ctgccctcct aaaaatgctt gtcaacgagc cgcaacacat caccccaggt
     1201 cttttgcaca acctcggcgg agacaagatc ggcagatgtg tgacagtgca gcgtccggtt
     1261 gaggcagagc acactggaca ccgtcttgag gatcacattg atgtcgtgcg aaaccagcag
     1321 taccgtccgt ccgtccgcgt tcagcttcga gaggagagag tagaaaccgg ctatctgctg
     1381 tgaatctact ccggcctccg gctcatccag aaacagatag cggcttccgc caacgatggc
     1441 ccgggccagc aagacgcgct gcaactcacc ccccgagagt gcgtcaatcg cccgatgctc
     1501 aagatgggcc gcaccggtca tctcaagggc ctcgtgcacc tgttgtgcag cattggccgg
     1561 taaccgggca aaggcgcccc ggcaggccag cagaccaaga cggacaatat ctgcggccgt
     1621 cccgggaaag aggcggggaa ccatctcccg ctggggaaga tacccgacat ggtttttcct
     1681 caaccattcc ctggccggga cgccgtcgta caaaacctgg cccgtgtgct ccggaacaag
     1741 tccgagcaag atcttgatca aggtggattt tccggcgccg ttcggcccga ggatagcggt
     1801 aaaatcaccg tccgaaagcg taaaactcac attctcaagg accgtcgatg tcccgatctt
     1861 cagaccgaca ctgcgcacct cgatcatcgt gcaaaccctt tggcaaacat ctcccagtta
     1921 taccggatca atgcagcata attcgtcaca ccgggatcct gtccgatcgg gtcgacgctg
     1981 tagaccggca acccgtattc ctttgccaga atattgacac tccgcggatc catctgtggc
     2041 tcacggtaga gcgcacgaac cccggcatcc cggatttttc tcccgagacc ggccagttca
     2101 gcagcagacg gctccttccc aggagtcgcc atcagggcag cgacccgtgt tattccatac
     2161 cgtgcaaaca atctggggaa ggcatcatga aaggtgacga ccgcccgtct cgcatatccg
     2221 gccgcctcgt cccggatccg tgcatcaagc gccatcacgc ggtttgaaaa ggagatcagg
     2281 ttattggcaa acaggtcccg gccttctgga tcatgctgtc cgaaccgttt tgccagcacc
     2341 ccggcaaacc gcaccatcag aaccggatcg gtccagagat ggggatccgt gttcctgcca
     2401 tgcccgtgac catcatcttc atgcccctcc gcatgcttct cctcccggcc cggagtgccg
     2461 aggatgtccg cggcaagaat cgtccgggcc gactggtcat ggaaaaaccg gcccagtttt
     2521 tcttcgagac ccaggccgtt ggcgatgatc aacccggccc ggctgatccg ctggacatcg
     2581 ctcggcctgg gcgaccaggt atgtggagag gctgaaacag ggagcaacgt ctcgacccgc
     2641 agtcgtgcac cggcaatctc ggccgcgatc aaggcatagg gacggatcgt ggccagcacg
     2701 agtctcttgt caatcccgtc accggaccgg ccgcaggcaa gcaccccgaa ccagaccaat
     2761 gtcacgatcc acactagctt tcgcatcctg ctgcctcccc atccatcgct cgaaaacctg
     2821 cacactcctg ccggtcgctg cattggcaca taagctgtgg gcctgccggc aagaactgtc
     2881 tgcaggagaa tatcgcaccg caggatggaa tcgtccagcg ttcccgccga cgtggcagca
     2941 tctgcaatgg atcattatca aacttgctgt caactttttg cgtcgtccga cgctcgttgt
     3001 gacgggcctg cacccccgac agcgccgacg ccatcaggtt ccaaacactc cctcgggcaa
     3061 aacatttcct cccgtcccga ggctgttcac cataaaaaag tcagaggctg aaaagaccgg
     3121 ccttcttccg gccaagcaaa cccagaatcc cgttgacaat ggtcagagga tgcgcgggac
     3181 atcccgggat atagagatcc ggtacatgct cctggaggaa ggtccggtcg agatcggggc
     3241 tgtcggcaaa gaccccgccc gaaatcgcac aggcaccggc cacgatcagg agtttcggct
     3301 cgggcatcat ctcccagaca tcccggacag ccggcgccat attccggctg accggaccgg
     3361 tcagaaaaag agcatcggca tgccggggac tggccaccac ctcgacgccg tatcgattca
     3421 aatcgaagtt gacattgtgc gacgcgttca gctccagttc gcacccgttg cagccccccg
     3481 cggagacgga ccgcagtttg agtgaccggc cgagaacttt gaggatctcc cggcccgcgg
     3541 gctccagccg ctccggccag tcttcaattc
//
LOCUS       DAOSHE010000208         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24343_length_3558_cov_4.232087, whole genome shotgun sequence.
ACCESSION   DAOSHE010000208 DAOSHE010000000
VERSION     DAOSHE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24343_length_3558_cov_4.232087"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..595)
                     /locus_tag="PLD82_06535"
     CDS             complement(131..595)
                     /locus_tag="PLD82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOG65078.1"
                     /translation="MEISTTLDPAQLAATRRQVEMHNAELKAKGINTEKDLGQDKYLK
                     LLVTQLRYQDPQNPLQNHEFAAQMAQFSALEQMTKMNSSMEKVIASARAGESYNMLGK
                     EVAWLDADTKEMQRGVVKAIVLADNRPMLRVGNIDVDPSKIASVSLPKAAGQ"
     gene            complement(671..2227)
                     /locus_tag="PLD82_06540"
     CDS             complement(671..2227)
                     /locus_tag="PLD82_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOG65079.1"
                     /translation="MQGYAEGVLLTGPLARSQTLLPPGFESRISPSQPAPQNSAFVGL
                     LADSLKPPDPVREERPPAPESFNAAVEPFEDEYEADLDGCAQREPEQVDAREERAIED
                     ARRFEEEERKRQRVPIFGEQAPVRDALAPARKEPVKEATGEIPPFLRDVARLVRTLKK
                     IVEESGDGTQAGPAAKRADEAIKGMQALLDLLRGVLPKQKMEAMERAPVALETALKEG
                     RTGKGLTQPGVVAKQVEEFLGKLPALQDLVAEASKQVKDARKPEGPPEKKPKGVLQAG
                     GADLRSIEDGIRMRLDVKDQRVNDPHSLREPVRIVKRQSVEGEAVQKSGEKKAPIPPP
                     ETGGLPPVKAPSSGREASTFQQDLASRMTQTVKKAAATETLTNPRQVVEQIVSRARVT
                     VKSGMTEMHLQLNPRNMGRVGIHFMVNQEGELTAKLMASNESVRQYLQENLSTFSRDL
                     ADAGVVVTRIEVSADAPGRQFPQEGRSGRDWAQDRDGAVPLTVRQEPRTITGGYSLQT
                     GSEGNLDVLA"
     gene            complement(2409..3023)
                     /locus_tag="PLD82_06545"
     CDS             complement(2409..3023)
                     /locus_tag="PLD82_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65080.1"
                     /translation="MKRDTRKIIMAVLLNLFLGAVLLYVLDMTGAMTFESIMTSLGLS
                     EGEAIKIEDRFLLEREELNKQWLVLSLKEKDYGEKLHQLTTNDQRLKDKENQLKGLEK
                     QLREREKKASEAEMEKIKRSERVRIVADQLMNMPPKDAVPRLEAQTDDLLVIDILRAM
                     DAVAADQGKRSIVPYLLSLMKGERAAAIQRKMANISTLSNEPEF"
     gene            complement(3201..>3558)
                     /locus_tag="PLD82_06550"
     CDS             complement(3201..>3558)
                     /locus_tag="PLD82_06550"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG65081.1"
                     /translation="RNTSQPARPGFAVIPPANTGIRFEPVRFTVGGRNCFGVRARVED
                     RHHNDPVLAGLHFARVLLRLYPGRFVMTRGFANEVGDPAIIERLKQGETPESIVASWK
                     PALDRFLAIRARYLLY"
BASE COUNT          648 a         1113 c         1001 g          796 t
ORIGIN      
        1 acggcccctc atgcaccgcc tgcgatctcg ctggcccgtg ggccagtccg gactcccgtg
       61 cctgcacagc tggtcgtgca gcgaactgtc tggcgatatg actggcatca agcatgcccg
      121 gcctcccccg tcattggccc gctgccttgg gcagggagac actggcgatc tttgacggat
      181 caacatcgat gtttccgaca cgcaacatgg ggcgattgtc ggcaagaacg atggccttga
      241 cgacacctct ctgcatctct tttgtgtcag catcaagcca ggcgacctct tttcccagca
      301 tgttgtagct ctcgccggca cgggccgaag caatcacttt ttccattgag ctgttcatct
      361 tggtcatctg ctcgagagcc gagaattggg ccatctgggc agcaaactcg tggttctgga
      421 gcggattctg cggatcctga tagcggagct gggtgaccag gagtttgaga tacttgtcct
      481 gcccgagatc cttctcggta ttgattccct ttgctttcaa ttcagcattg tgcatctcga
      541 cctggcggcg tgtggcagcc agctgggccg gatcaagtgt tgtgcttatt tccatatcgg
      601 tttttcctca ctttccggat ttttttggtg aacaacctca ggccagaaaa tcgcccaggg
      661 ttctcccgat tcatgcgagc acatcaaggt ttccctcgct tcccgtttga agcgaatatc
      721 caccggtgat ggtgcgcggt tcctgccgca ccgtcagggg aacggccccg tccctgtcct
      781 gtgcccagtc gcggccactc cgtccttcct gggggaactg gcgaccggga gcatcggcgc
      841 tgacctcgat ccgtgtcacg acgacaccgg catccgccag atcccgggag aaggtggaca
      901 agttttcctg gaggtactgg cgcaccgact cgttggaggc catcaacttg gccgtcaatt
      961 caccttcctg attgaccata aaatgtatcc cgacccgccc catatttcgt ggattcaact
     1021 ggaggtgcat ctcggtcatc ccgctcttga ccgtcacccg cgcgcgggaa acaatctgct
     1081 cgaccacctg cctgggattg gtcagggtct ccgtggctgc cgctttcttg acagtctgcg
     1141 tcatcctcga agcgagatcc tgctggaatg tcgaggcttc ccgaccggat gacggggcct
     1201 tgaccggggg aagtcctccg gtttccggtg gcgggatcgg cgccttcttt tctccggact
     1261 tctggactgc ctcgccctcg accgactggc gtttgacgat gcggacgggc tcacggagag
     1321 aatggggatc gttgacacgc tgatccttca catcaagacg catccggatg ccatcctcga
     1381 tggaccgcag atccgctcca cccgcttgaa gcacgccctt gggctttttc tccggcgggc
     1441 cttccggttt cctggcatcc ttgacctgtt tcgatgcctc ggcgaccaga tcctgcagag
     1501 ccggaagttt tccgagaaat tcctcgacct gtttggcaac aacccccggt tgcgtcagtc
     1561 ccttgcccgt ccgcccttct ttcagggcgg tctcgagtgc gacgggagcc ctttccatgg
     1621 cttccatctt ctgtttgggc aaaacaccac gcaacaggtc cagcagtgcc tgcatcccct
     1681 tgatggcttc atcggcgcgc tttgccgcgg gtccggcctg cgtcccgtct ccgctctcct
     1741 ccacgatctt tttgagcgtg cgcaccaggc gcgccacatc ccgcaggaat ggcggaatct
     1801 ccccggttgc ttccttgacc ggctccttgc gcgccggagc cagcgcgtca cggacgggag
     1861 cctgttcacc aaaaatcggc acccgctgac gcttgcgctc ctcctcttcg aagcgccgcg
     1921 catcctcgat ggcccgctcc tcccgtgcat ccacctgttc cggttcacgc tgcgcacacc
     1981 cgtcgagatc cgcttcatac tcatcctcaa acggctcaac ggctgcattg aacgattccg
     2041 gcgccggtgg acgctcttcg cggaccggat ctggcggctt gagcgaatct gccagcaaac
     2101 cgacaaatgc cgagttctgc ggtgccggtt gcgatggact gatccggctc tcgaaacccg
     2161 gcggcaagag cgtttggctg cgagcgagag ggcctgtcag caagacacct tctgcatacc
     2221 cctgcattcc ttcctccttt caagagtctt ttcccaagca tcggaagaaa tcgctgactc
     2281 attgacgagg attattcagg agacccgcta gacaggcgtc ccgggtcaaa accggcagaa
     2341 cggactgata acgggatgga tacgggaaac ctgccccggg caacttcaat ccagaggaca
     2401 aaccgggatc agaactcggg ttcgttggag agtgtcgaga tattggccat cttgcgctgg
     2461 atcgcagcag cacgctcgcc cttcatcagg gagaggaggt agggcacgat ggagcgtttg
     2521 ccctggtcgg cggcgacggc atccatggcc cgcaggatgt caatcacaag gaggtcgtcc
     2581 gtctgggcct cgagacgggg gacggcatcc ttgggaggca tattcatcaa ctggtcggcg
     2641 acaatccgga cccgctcgct gcgcttgatt ttttccatct cggcctcgct ggccttcttt
     2701 tcgcgctccc gcaactgctt ttcaaggccc ttgagctggt tttccttgtc cttcagtcgc
     2761 tggtcattgg tcgtaagctg gtgcagcttc tcgccgtaat ccttttcttt gagggagagc
     2821 acaagccatt gtttgttgag ctcttcccgt tcgagaagaa acctgtcttc gatcttgatg
     2881 gcctctccct cggacaagcc cagggatgtc atgatgctct cgaaggtcat cgcccccgtc
     2941 atgtcgagga cgtagagcag gacggccccc aggaagagat tgaggagtac cgccatgatg
     3001 atcttgcgtg tgtcgcgctt cattccggtc cccgttcgct ggtctttcca cataataata
     3061 gcattgctgc gccatgacac gcaagcggga tgtgtctggc acggtatcaa ccgggaatgc
     3121 caaaccctct tcggtgacac tccggttcac gcaggcctga ggctgttcac caaaaaaaac
     3181 cgtggccggt accaggctga tcaatagaga agatagcggg cacggatcgc gagaaacctg
     3241 tcaagggcag gcttccagga tgccacgatc gactcgggtg tctcgccttg tttgagtctc
     3301 tcgataatcg ccgggtcccc gacctcgttg gcaaaccccc gggtcatgac aaaccgcccg
     3361 ggatagagcc gcagcaggac acgggcaaaa tgcaaaccgg ccaggacggg gtcattatgg
     3421 tgacgatcct caacccgggc ccggacaccg aaacaattcc gcccgcccac ggtaaaccgg
     3481 actggctcaa accgtatccc ggtgtttgcc ggaggtatca cggcgaatcc gggacgggcc
     3541 ggttgcgaag tattccgg
//
LOCUS       DAOSHE010000209         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24353_length_3557_cov_3.753284, whole genome shotgun sequence.
ACCESSION   DAOSHE010000209 DAOSHE010000000
VERSION     DAOSHE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24353_length_3557_cov_3.753284"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1721
                     /gene="atpA"
                     /locus_tag="PLD82_06555"
     CDS             195..1721
                     /gene="atpA"
                     /locus_tag="PLD82_06555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG65082.1"
                     /translation="MGMKDFSFESIQQKIERFDIEASSDESGQVIESADGIARVRGLP
                     HVENGEMVEFPNDIYGLVFNLEKDEVICAILERHTAVREGSWVKRTGRVMEIKVGEGL
                     LGRVIDPIGNPMDEKGPVATNAAYPMERSAPGVVERQPVRVPLATGYKIVDSLVPIGR
                     GQRELIIGDRQTGKTTLAVDAIINQKDTGVISVYVAIGQKQATVVKLVADLARHGALE
                     NTVIVNAPASSPAITQYLAPYAGCAIAEYFMYEKKRDTLVIYDDLSKHAAAYRHISLL
                     MRRPPGREAFPGDVFYLHSRLLERAAKLSDDLGAGSMTALPIIETQGNDISAYIPTNV
                     ISITDGQIFLEADLFNAGVRPAVNAGISVSRVGGSAQQKMMRQVSGTLRIDLAQFREK
                     EAFSMFSSDLDAETIAQLKKGKLMVELLKQNKGGPMATVDQVISLYAGTRNLISDVPS
                     ESVDEFEKGLLARFQEKHGDVRRSLVEKGSIDKDLEETLRTIILEYKKEFLDGSASRN
                     "
     gene            1699..2619
                     /locus_tag="PLD82_06560"
     CDS             1699..2619
                     /locus_tag="PLD82_06560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HOG65083.1"
                     /translation="MAQLLAIKRRIRAIEGLRKVTRAMELVTHTRIGRVRRGAENARR
                     YHESFARLMKRVALAMDEDPASGEKTGPGGSSAPRHELVIFLSQKGFCGAFNDRLLAR
                     VARDLPRMGAIGITVVGRLSPKWNSIMRRKPNSLLEAPDKSWQRDTASLAAGWARDIA
                     NGSLGRVVFAFNRFVSILEQTPQFVQVWPPEETVGESVSAEAADDPDGISSWPLVEPD
                     AATLFTSLTGPWIEACLARAWWETAAGENCSRLISMKSANENAATILDDLRLRYNKTR
                     QLVITQELAEISSAFNVLNILKQKREQSED"
     gene            2623..>3557
                     /gene="atpD"
                     /locus_tag="PLD82_06565"
     CDS             2623..>3557
                     /gene="atpD"
                     /locus_tag="PLD82_06565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG65084.1"
                     /translation="MANNRGKILQVVGPIIDIKFSKEFIPSIRNAIEIHHTASNGTTR
                     TVIAEVSMQMEGGIVRCVSMAPTEGLARGMEAIDTGKPILVPVGQKVLGRLFNALGQP
                     LDGLGDFGEESPTLPIRNNPPSFASIEPRDTVFETGIKVIDLMSPYVKGGKTGLFGGA
                     GVGKTVIIMELIHNMATKHGGISVFAGVGERTREGNDLWLEMKESGVIDKTALVFGQM
                     GELPGARMITPLTALTMAEHFRDNEHQDVLFFMDNVFRFVQAGSEVSTLLGRMPSAVG
                     YQPTLALEMGRLEERIVSTRNGFITSIQAVYVPAD"
BASE COUNT          795 a         1081 c         1031 g          650 t
ORIGIN      
        1 gggtcgcagc caaactccgc gaactcttcg gcaagcaggc cttcgtctat aacaaggtct
       61 cccgctattt ctcggggggg ctcctgatcg agtgcgacaa tacaatgatc gatctcgggg
      121 ttcgcaccag tctttaccag ttaaaacagg cgctcggggc gtcttgaggg aaaagtgagg
      181 tttgaacagg caccatggga atgaaagact tttcgtttga gagcattcaa cagaaaatcg
      241 agcgctttga catcgaggcg agttcggacg aatcgggaca ggtcatcgaa tctgccgacg
      301 gcatcgcccg ggtccgcggg ttgccccacg tcgaaaatgg cgagatggtc gagtttccca
      361 atgacatcta cggactcgtc ttcaacctcg aaaaagatga agtgatctgc gccattctcg
      421 agcggcatac cgctgtccgc gaagggagct gggtcaagcg caccggccgg gtgatggaga
      481 tcaaggtcgg cgagggcctc ttgggtcgcg tgatcgaccc gatcggcaac ccgatggacg
      541 aaaaaggacc ggtggccacc aacgcagcct acccgatgga gcgttccgct cccggtgtgg
      601 ttgaacgcca gccggtccgc gttcccctcg caaccggata caagatcgtc gacagtctgg
      661 tgccgatcgg acggggacag cgcgaactga tcatcggcga ccgacagacc gggaaaacga
      721 ccctggccgt cgatgcgatc atcaaccaga aagatacggg tgtcatctcg gtctacgtgg
      781 ccatcggaca gaagcaggca acagtcgtca agctggtcgc cgatctggca cgccacggag
      841 cccttgagaa cacggtcatt gtcaacgccc cggcctccag cccggcgatt acccagtacc
      901 tcgcacccta cgccggttgc gccatcgccg aatacttcat gtacgaaaaa aaacgggaca
      961 ccctcgtgat ctacgacgat ctgtcgaagc acgctgcggc ctaccgccat atctccctcc
     1021 tgatgcgccg cccccccggg cgggaagcct tccccggtga tgtcttttac ctgcactccc
     1081 ggcttctcga acgggcggca aagctctcag acgatttggg ggccggttcg atgaccgcgc
     1141 ttccgatcat cgagacccag gggaacgaca tctcggccta catcccgacc aacgtgattt
     1201 ccatcaccga cggacagatc ttccttgagg cggatctctt caatgccgga gtccgcccgg
     1261 ctgtcaacgc cggtatctcc gtctcccgtg tcggcggcag tgcccagcaa aagatgatgc
     1321 gtcaggtttc ggggaccttg cggattgacc tcgcccagtt ccgtgaaaaa gaagccttct
     1381 cgatgttctc ctccgacctg gatgccgaga caatcgccca gctcaaaaaa gggaaactga
     1441 tggtcgagct tctcaaacag aacaagggtg gcccgatggc gaccgtcgat caggtcatct
     1501 cgctctacgc cggaacccgc aacctgatca gcgacgtacc ctccgagtcc gttgacgaat
     1561 tcgagaaggg actcttggcc cgtttccagg agaagcatgg tgacgtccgc aggagcctgg
     1621 tagaaaaggg cagcattgac aaggacctcg aagagaccct gcgcacgata atccttgaat
     1681 acaaaaagga attccttgat ggctcagctt ctcgcaatta aacggcgcat ccgggccatc
     1741 gagggactgc gcaaggtcac ccgcgccatg gagctggtaa cccatacccg gatcggccgg
     1801 gtccggcgcg gggccgaaaa cgcccggcgc taccatgagt cctttgcccg cctgatgaaa
     1861 cgggtggcac tcgccatgga cgaggatccg gcatccgggg agaagaccgg ccccggagga
     1921 tcgtccgccc cccgccatga actggtgatc tttctgtccc aaaagggatt ttgtggcgca
     1981 ttcaacgacc gtctcctggc acgcgttgcg cgtgatctcc ccagaatggg cgcaatcggg
     2041 attaccgtgg tgggacggct ttcgccgaaa tggaactcga tcatgcgccg caaacctaac
     2101 tcgctccttg aggctcccga caagagctgg cagcgcgaca ccgcgagcct ggccgcaggc
     2161 tgggcccgtg acatcgccaa cggatcgttg gggcgggtcg tcttcgcctt caaccgcttc
     2221 gtgtccatcc tcgaacagac accgcagttt gtccaggtct ggccaccgga ggagacggtg
     2281 ggcgaatccg tttccgccga agccgcggat gatcccgacg ggatctcgtc ctggcccctc
     2341 gtggagccgg acgcggccac cctcttcaca agcctgaccg gaccttggat cgaagcctgc
     2401 ctggcccggg cctggtggga gacagccgcc ggagaaaact gctcccggtt gatcagcatg
     2461 aagagtgcca atgaaaacgc ggccacaatc ctggatgatc tcaggctgcg atacaacaaa
     2521 acacgccagc tcgtcatcac gcaggaactg gccgaaatct cgagcgcgtt caacgtgctc
     2581 aacatactca aacagaaacg cgaacagagc gaggactgag cgatggcaaa caaccgcgga
     2641 aaaattttgc aggtcgtcgg accgatcatc gatatcaaat tcagcaaaga attcattccg
     2701 tcaatccgga acgcgatcga gatccaccac acagcatcaa acggaacaac ccgcacggtg
     2761 atcgccgaag tctccatgca gatggaggga ggaatcgtgc gttgtgtctc gatggcaccg
     2821 accgagggct tggcccgcgg gatggaggcc atcgataccg ggaaaccgat cctggtccct
     2881 gtcggacaaa aagtcctcgg ccggctcttc aacgcgctgg gacagccgct ggacgggctg
     2941 ggcgattttg gtgaagagag cccgacgctt ccgatccgca acaatccccc ctccttcgcc
     3001 agcatcgagc cacgcgatac ggtgttcgag accgggatca aggtgattga cctgatgtcc
     3061 ccctatgtca agggaggaaa gacgggactc tttggtggtg ccggtgtcgg gaaaacggtc
     3121 atcatcatgg aactgatcca caatatggcg accaaacatg gcggaatttc ggttttcgcc
     3181 ggcgtcggag aacggacccg cgagggaaac gacctctggc tggaaatgaa ggaatcgggg
     3241 gttattgaca aaaccgctct tgtcttcgga cagatgggag aactccccgg agcccgcatg
     3301 atcaccccgt tgaccgcgct gacaatggcc gaacatttcc gcgacaacga acaccaggac
     3361 gtgcttttct tcatggacaa cgtcttccgc tttgttcagg ccggcagcga ggtctcgacg
     3421 cttcttgggc ggatgccctc cgcagttggc taccaaccaa ccctggccct cgagatgggc
     3481 cgccttgagg aacggattgt ttcaacacga aacgggttca tcacgtccat tcaagccgtc
     3541 tatgtcccgg ccgatga
//
LOCUS       DAOSHE010000210         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24466_length_3544_cov_3.681857, whole genome shotgun sequence.
ACCESSION   DAOSHE010000210 DAOSHE010000000
VERSION     DAOSHE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24466_length_3544_cov_3.681857"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="lepB"
                     /locus_tag="PLD82_06570"
     CDS             <1..989
                     /gene="lepB"
                     /locus_tag="PLD82_06570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG65085.1"
                     /translation="LIRVFLVDNYQIPSGSMIPTMLEGDRLFVSKFNYGVRLPILPNW
                     KLPRFDSPDRGDIVIFQYPNYRSPGWLAELADLFTFSIFGLDPMEKNFVKRAIGLPGD
                     FIALEENGTLWLNGKQVPRRKLYKRIVRGKEESGVFEVFASTNESVTNKLYEYSPGRN
                     VYPVGDFEVWEERLGGNTYQVQYSMHAKREKFPPRHQEGWHFFLIEVQKVWLEKNMLQ
                     SSQNETNELVKVFTKQQYDALPAGKVANCLVHAKDGQVWYRVHGMTNIAPLISMYSNR
                     LCIVVPKDHYFVMGDNRDNSSDSRVWGFLHKDYLMGSPLIRYLPFSRFGTID"
     gene            1134..1568
                     /gene="lepB"
                     /locus_tag="PLD82_06575"
                     /pseudo
     CDS             1134..1568
                     /gene="lepB"
                     /locus_tag="PLD82_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            2187..2339
                     /gene="lepB"
                     /locus_tag="PLD82_06580"
                     /pseudo
     CDS             2187..2339
                     /gene="lepB"
                     /locus_tag="PLD82_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            complement(2491..3051)
                     /locus_tag="PLD82_06585"
     CDS             complement(2491..3051)
                     /locus_tag="PLD82_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOG65086.1"
                     /translation="MIDSPNAHRLHAVTCPDDIFPAYRDTPISLLLEYHNLERQFDTW
                     DKAQVLIGMCMDNRKQLRIPDNFAYIIRSGGGNLRPSEFKVSYAIGVGGVTSIALLAH
                     DNCGMVNVQTKRELFVSGLVENAGWTREAAEAHFNSFSPLFEIGESIDFVLSEAKRLR
                     ERYPKILVAPLFYNIADRRLALIHEE"
     gene            3301..>3544
                     /locus_tag="PLD82_06590"
     CDS             3301..>3544
                     /locus_tag="PLD82_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65087.1"
                     /translation="MMFACAKVDYAYRHDVAGRVVDGQTGRAVNNAIVERIEEGVPTD
                     AVRDLYRRVTGPDGSFRFEYSGLGGKPEQCQIWMLQV"
BASE COUNT          810 a          867 c         1010 g          857 t
ORIGIN      
        1 tcctgatcag ggtcttcctg gtggacaact accagattcc ctccggttcg atgatcccga
       61 ccatgctgga gggggacagg ctctttgtct ccaagttcaa ttacggggtc cggcttccga
      121 ttctccccaa ctggaagctc ccgcgctttg attccccgga tcggggggat attgtgattt
      181 tccagtatcc caattaccgt tcgccgggct ggctggccga gctggccgac ctcttcactt
      241 tttccatctt cggactcgat ccgatggaaa agaactttgt caaacgggcg attggcttgc
      301 ctggtgactt catcgcactc gaggagaacg ggactctctg gctcaacggg aagcaggtgc
      361 cacggcggaa gctgtacaaa cgaatcgttc ggggaaaaga ggagagcgga gtgttcgagg
      421 tttttgcatc gacgaacgaa tccgtcacga acaagctcta tgagtattca cccggacgga
      481 atgtctatcc ggtgggggat tttgaggtct gggaagaaag actgggcggg aatacatacc
      541 aggtccagta ttcgatgcat gcaaagcggg agaagtttcc accacggcac caggagggct
      601 ggcatttttt tctcatcgag gtgcaaaaag tctggctcga gaaaaacatg ctccagtctt
      661 cgcaaaacga aacgaacgaa ctggtcaagg tcttcacaaa gcagcagtat gatgccttgc
      721 ctgcaggcaa ggtcgcaaac tgtctcgtcc atgcaaaaga tggacaggtt tggtaccgag
      781 tgcacggaat gacaaatatt gcgccactga tttccatgta ttccaacagg ctctgcattg
      841 ttgttcccaa ggatcattat tttgtgatgg gcgacaatcg ggacaacagt tccgacagcc
      901 gggtttgggg ttttttgcac aaggattacc tgatgggcag tcccctgatc cgttatctcc
      961 cgttttcacg tttcgggacg atcgactgag gctggttgta caggctggca aagacccggt
     1021 cgtcctatac cggaatgaac aaacgggatt tcaggggaga atacatgcag ttcagcatct
     1081 ggaaacgcaa ggagcagaaa ccggacctga ccgagggtga atccggcggc agaatgaaaa
     1141 aaaagggcag gattcgggaa aatatcgagg tgatcctctc cgcaattctg attgccgtcc
     1201 tgatcagggt ttttttggtg gataactacg agatcccttc gggttcgatg attccaacca
     1261 tgcttgaggg ggacagactc tttgtttcca aattcaatta tggcgtccgg cttccgattc
     1321 ttcccaattg gaagctcccg cgctttgatt ctcctgatca tggggatatt gtgatcttcc
     1381 agtatcccaa ttatcgctcg ccgggctggt tgtccgagtt ggcggatctt ttcaccttct
     1441 ccatttttgg tctcgatccg atgccgaaga attatgtcaa gcgggtcatg ggcttgccgg
     1501 gtgaccatgt acggatcgat ccggacggca cggtctggag aaacgggaaa cctgtcggga
     1561 gacggttttt tgaggaacgg gctcttgtcc gcctgcctgc cgatggtgcc atgaacaagc
     1621 atattgtccg atatctgagt ggtaccaatc tggtctacga gaatactgtc gccggctcga
     1681 ttccctcgtg gtacgatccc cgcaatccgg aaatgtacag gatctatcgc gaaggggatg
     1741 ccggaagtga gtaccttgtc cagtatttga cggacggttt tttgcaggaa gagcgcaaag
     1801 agacgatccg gaccatgctc agaccatttc ccctgggccc gaaggatttc gaggccaata
     1861 tccatgcccg gtccatgtat accaacgaca tcaaaaattt ctacaagaag attgcgaata
     1921 tctggcttga acagaacatg ctgcaatcat cctactatat ggatgtcctg gacagggaaa
     1981 cctaccgccc caaaaaaatt ctcctgggtc ccgacgacag gatggcaact gtggatctct
     2041 attcgatgga acagtatgca cagatcacgg cgaagacctc cgatctggat tgtcttgtgt
     2101 acgcaagctc gggtgaagcc tggtacagag tcaaggggat gaccaatctc cagcctctct
     2161 ttgccatcta ttccaacagg ctctgtgtgg tggtcccgaa ggaacattat ttcgtgatgg
     2221 gggacaaccg tgacaacagc tccgacagca gggactgggg gtttctgcac aaggactacc
     2281 tgatggggag tcccttgatt cgctatcttc cgttctcccg cttcgggacg atggactaga
     2341 gcgggtaatt tttgcgcact ttctggcgta gatctgcaga attgccgtgg tattcccgga
     2401 atccgataat aaaaacgtga tactgtcatg tgactccggg agtattctga tacaaagcga
     2461 tgtgaccgtg gtattcccga cttacgggtc tcattcctcg tggatcaggg ccagcctgcg
     2521 gtcggcaata ttgtaaaata gtggagcaac cagaattttc gggtagcgct cccgcaggcg
     2581 tttggcctca ctcaggacaa agtcgatgga ttccccgatc tcgaacaggg ggctgaagct
     2641 gttgaagtgg gcctctgcgg cttcccgtgt ccagccggcg ttttcgacca gccctgatac
     2701 aaagagttcc cgtttggtct gcacattgac catcccgcaa ttgtcgtggg ccagcagggc
     2761 aatggaagtg acaccgccga ctccgatcgc gtaggagacc ttgaattcgc tcgggcggag
     2821 attgccgcca ccgctgcgga tgatgtaggc gaaattgtcc gggatgcgga gctgtttccg
     2881 gttgtccatg cacatcccga tcaggacctg cgccttgtcc caagtgtcaa actggcgctc
     2941 gaggttatgg tattccagaa gcaggctgat gggggtatcc cggtacgcgg gaaaaatgtc
     3001 gtccgggcag gtgacggcat gcagacggtg tgcgtttggc gagtcgatca tggcaaaaac
     3061 ttactgagac ccccgaaatt gtcaagcggt atccccgcgt gtgtcaacgc gtgaggatag
     3121 tcaaaggaca atccgggaca gatattacag ttatgtaatc tctatcccgg attcgcataa
     3181 ttcttttgca atattcaggt gtgttgcggc acaaatcggg ctattatggg tgtgtactct
     3241 ctgaaagcct ggaggtgaat cgtgtcatat aggactggaa tgctgggaat agcgtgcttg
     3301 atgatgtttg cctgtgccaa agtggattac gcctaccggc acgatgtggc cggccgggtt
     3361 gttgacggac aaactggccg ggctgtcaac aatgcgattg ttgagcgaat tgaggaaggt
     3421 gtgccaacgg atgcagttcg ggatctctac cgtcgagtga cgggcccgga tggcagcttc
     3481 cggtttgagt acagtggtct gggtggaaag cccgaacagt gccagatctg gatgcttcag
     3541 gtcc
//
LOCUS       DAOSHE010000211         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24707_length_3522_cov_3.428324, whole genome shotgun sequence.
ACCESSION   DAOSHE010000211 DAOSHE010000000
VERSION     DAOSHE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24707_length_3522_cov_3.428324"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLD82_06595"
     CDS             complement(<1..322)
                     /locus_tag="PLD82_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG65088.1"
                     /translation="MFKPVDTDVSFPKLEEEVLAFWREHHTFEKSLEWRKGAPEYSFY
                     DGPPFATGLPHYGHILPGTMKDIIPRYQTMKGRYVPRRFGWDTHGLPVENEIEKKLDL
                     KTKRD"
     gene            complement(506..1384)
                     /locus_tag="PLD82_06600"
     CDS             complement(506..1384)
                     /locus_tag="PLD82_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG65089.1"
                     /translation="MKKPFFQRNPFRRAAHSAGLALASGGAKGVAHIGVIESLEEMGI
                     TIECVAGSSIGAVVGAVYVCGSLKRFREAMLAMTRRKMFQLVDPVFPKSGFIRGDKLI
                     EFLGDFIPPGVRFEDLPIPLAVVATDFGTGQRAVFTQGLVLPAVRASMSIPGVFEPVE
                     IGGRIYVDGGAADVLPADLPRRLGARRVIAVGLHPPPEGSLGTVMSEERFLEVPPKGY
                     TRAKKSPGVVSVVMRSIDIMDHATEAASIAADKPDVLILPEIHQIGTLEFDQSTAAIG
                     AGRAAAAARSREIARL"
     gene            1467..1874
                     /locus_tag="PLD82_06605"
     CDS             1467..1874
                     /locus_tag="PLD82_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65090.1"
                     /translation="MKRIVLSLTLVAALLAACGGPSNETPQDLVQTLLKTIAKNDMET
                     YSTYAVYYDRAKVLDAFLKARESGARAGVNWDDIKIRNIEMESDDDYVATFESAGKMF
                     SVKFYSIEKSEKGFVKKTSVIGSFRKVRTSLDN"
     gene            1880..2413
                     /locus_tag="PLD82_06610"
     CDS             1880..2413
                     /locus_tag="PLD82_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOG65091.1"
                     /translation="MISPETDFNQETLEQIARQMALAARTAPKAKGHDQLEILIVTGT
                     DKDALANRMMEIAERQDAPSFLRDSKNIRVCGVILLIGTKTRQIGLKYCGLCGYANCA
                     AMAAGPGGVCVFAANDLGIATGSAVALAADHRIDTRIMYTAGIAALEQGLFSPEVRIA
                     WAIPLSASGKSPFFDRG"
     gene            complement(2544..3356)
                     /locus_tag="PLD82_06615"
     CDS             complement(2544..3356)
                     /locus_tag="PLD82_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG65092.1"
                     /translation="MKHITDFHTHAFADRIYERAMQALKEGSGIEPFHNGSLAGLLES
                     MDQAGIWRSVVLSIATKPEHADVILDWSLLIASERIIPFGSVMPTDRNAARDVRRMAG
                     AGIKGIKIHPYYQNFYMDDTAMDPFYEAVAESGLILVSHTGYDVAYPGRDDLATPERT
                     ARVIARHPDLCLVAAHFGGWEDWDAVEKHLLGKPVWLETSFLTGVCDRERARRMFLSH
                     DPARLVFGSDSPWVGQAEEIQRVEGLDLPPDLLEAVMEKNAQALLDAAVHRA"
BASE COUNT          815 a         1034 c          938 g          735 t
ORIGIN      
        1 tatcacgttt tgtcttgagg tcgagcttct tttcgatctc gttttcaacc ggaagcccgt
       61 gcgtatccca gccaaagcgc cggggaacat agcggccctt catggtctgg tagcgcggaa
      121 tgatgtcctt catcgtcccg ggaagaatat gcccatagtg cggcagtccc gtcgcaaacg
      181 gagggccgtc ataaaaagag tattcaggtg ctcccttgcg ccattcgagg gatttttcaa
      241 aagtgtggtg ctcacgccag aaggcgagga cctcttcttc gagtttgggg aaggaaacat
      301 ccgtatcaac cggtttgaac atgtctgcct ctgtctgtct tccagccccc tgacaggaac
      361 aaacagggga cattgcgggt atcatctcta atttaggcag gcatggcagt tcaagacaag
      421 acagcaagac aggtttgagg aaccgcatcc ggcccattgg atccggctgg gacaaaaaaa
      481 ggaagccggc cggaggggtt ccccgtcaga gtctggcaat ttcccgcgac cgggccgcgg
      541 cggcggcacg accggccccg atggcagcag tcgattggtc aaactcgagg gttccgatct
      601 ggtgaatctc cgggagaatc aggacatccg gtttgtcggc ggcaatcgac gcggcctcgg
      661 tcgcatgatc catgatatcg atggagcgca tgaccaccga gacgaccccc ggggattttt
      721 tggccctggt gtatcctttg ggcggaacct cgagaaagcg ttcctcgctc atgaccgttc
      781 ccagggaacc ttccggcgga ggatggagac cgacagcaat cacccggcgg gctccgaggc
      841 gcctcggaag atccgccgga aggacatccg ccgcaccgcc atcaacatag atccgaccac
      901 cgatctcgac cggttcaaac actccaggaa tggacatgga ggcgcgtacg gccggaagga
      961 ccaggccctg cgtaaagacc gctcgctgcc ccgtcccgaa atccgttgca acgacagcca
     1021 ggggaatcgg gagatcctca aagcggactc ccggcggaat gaagtctccc aggaattcga
     1081 tgagtttgtc cccgcgaatg aagccgctct tcggaaaaac aggatcgaca agctggaaca
     1141 tctttcgccg ggtcatggcc agcatggcct cccggaatcg tttgagggaa ccacaaacat
     1201 agacggcgcc gacaacggcc ccgatactcg aaccggcaac gcattcgatg gtgatcccca
     1261 tctcttcaag agattcgatg acaccgatgt gggccacacc tttggcacca cccgaggcca
     1321 gtgctaatcc ggccgagtgt gcggcacgcc ggaacggatt tcgttgaaaa aacggtttct
     1381 tcatcccttc aatttattga caaatccgga taccggcata tacttgcaga atccgatttt
     1441 catccactac cctcaaagga gagaacatga aacgaattgt gttgtcgctg acactggttg
     1501 ctgcactgct ggctgcttgc ggtggcccaa gcaacgaaac accccaggac ttggtgcaaa
     1561 cgcttctcaa aaccattgca aaaaatgaca tggaaacata cagcacatat gccgtgtatt
     1621 atgacagggc caaagtcctg gacgcctttc ttaaagcccg cgagtccgga gcacgtgccg
     1681 gcgtcaattg ggatgatatc aaaatccgca atatcgagat ggaaagtgat gatgactatg
     1741 tcgccacatt cgaatccgcc ggaaagatgt tcagcgtcaa attctactcg atagaaaaat
     1801 ccgaaaaagg ttttgtcaaa aagacttccg tcatcggctc attccgcaag gttcgtacat
     1861 ctttggacaa ttgacctcca tgatcagccc ggaaaccgat ttcaaccagg agactctcga
     1921 gcagatcgcc cgtcagatgg ctcttgcggc gcgaactgct cccaaggcca aaggtcacga
     1981 ccagctcgag atcctgatcg tgacaggaac cgacaaggac gcccttgcga accggatgat
     2041 ggagatcgcc gagcggcaag atgcacccag ctttttgcgt gacagcaaaa acatccgcgt
     2101 ctgtggtgtc atcctcctga tcgggacaaa aacccgccag atcggtctga agtattgcgg
     2161 gctctgcggc tatgccaact gtgcagccat ggccgccgga ccgggcgggg tttgcgtctt
     2221 tgccgccaat gacctgggga ttgccacagg ctccgccgtt gctctggccg ccgatcaccg
     2281 gattgacacc cggatcatgt acaccgccgg gatagccgcc ctcgaacagg ggctcttttc
     2341 gcccgaggtc aggattgcct gggccatccc gctttcggcg agcggcaagt ccccgttttt
     2401 tgatcgcggg tagactatgc tgttgtactg aaccggtcgt acaagcctgt atttgtaccc
     2461 gggggggaca ctgcacatca agacctggct ggagatcatt cccggccagg tcgttacttt
     2521 gtgactcgtg aagctcccgg acttcacgcc cggtgaaccg cagcatcaag caaggcttgt
     2581 gcattctttt ccatgaccgc ttccagaaga tcagggggaa gatcgagtcc ttcaacccgt
     2641 tgaatctcct cagcttgccc gacccagggt gaatcggatc caaaaaccag gcgtgccggg
     2701 tcgtgtgaca gaaacatgcg tcgcgctctc tcccggtcac acaccccggt caaaaacgat
     2761 gtctccagcc agaccggttt accgagcaga tgcttttcca ctgcgtccca atcttcccat
     2821 cccccgaagt gtgccgcaac cagacacaag tccggatggc gcgcaatgac cctggctgtt
     2881 ctctcgggag ttgccagatc atcccgtccg ggataggcca catcgtatcc ggtatgggag
     2941 acgagaatga gtcccgactc ggccacagct tcataaaacg gatccatggc cgtgtcgtcc
     3001 atgtaaaaat tctggtagta gggatggatc ttgataccct tgatccctgc ccccgccatc
     3061 cttcggacat ctctggccgc attccggtcc gttggcataa ccgacccgaa ggggatgatc
     3121 cgctcacttg cgatcaagag agaccagtcc agaatgacat ccgcatgttc cggctttgtc
     3181 gcaatggaca aaactacaga gcgccagatc ccggcctggt ccatcgattc cagcaaaccg
     3241 gcaagtgatc cgttatggaa aggctcgatg cctgaaccct ctttgagtgc ctgcatggcc
     3301 cgctcgtaaa tccggtccgc aaaagcatgt gtatggaaat ccgtgatgtg tttcatgatg
     3361 atctgctcca gtcccttctc caacttcctc aaaacaggcg acgccaaaaa attcccgcag
     3421 cctctcaagt tcccggcaac tccggacgat atgtattcta tcggtggttg cggacttccc
     3481 tccctttgat ccgcattcac catcaggttt ctttaccgta ca
//
LOCUS       DAOSHE010000212         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24791_length_3513_cov_4.717178, whole genome shotgun sequence.
ACCESSION   DAOSHE010000212 DAOSHE010000000
VERSION     DAOSHE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24791_length_3513_cov_4.717178"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLD82_06620"
     CDS             complement(<1..1003)
                     /locus_tag="PLD82_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG65093.1"
                     /translation="MQEEQTVLIIGAGRLQVPVIKKAKEMGMHTVATDYSTSAPGLAL
                     SDDRIIISTRDIDGSVREARRYHREIRHIDGVLTVGTDASMTVAAVANALGLPGIRFI
                     NAEAASNKLKMRSRLHEHAVPCPRFGECWTLDDALDHFNRLGRGPVVLKPSDNMGARG
                     VSRADDEAGIRSAFAFAKEATPSGEVIIEEYMEGPELSIDALVYRGEIHITGVADRLI
                     ARPPWFVETGHIMPSNLPPAMQEDAIRVFVAGIKALGIDMGAAKGDIKVTPTGAMVGE
                     IAARLSGGFMSAYTYPYSSGVDLMANAIRIALGQPPRGLEEKLGRVSMESAIIPPPGR
                     "
     gene            complement(1096..2142)
                     /locus_tag="PLD82_06625"
     CDS             complement(1096..2142)
                     /locus_tag="PLD82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG65094.1"
                     /translation="MKRSSIWILVAVTSLSLVTILSGYEASHLNNLKRGVEYWNTWRK
                     HTRFANTDLSGAVLTNIGLWRVEMAYTDLSRSVLRNADIRFSNLTEAKLNDSDLERIV
                     LDGTILLYVDLRRSNLSRARLVDANLQRADLQQVNLTGASLTNTDLRLANIQFANLSN
                     AVFSACDFRGANLMGSRIVQTSFADSDLSQVKLNFARFDQANLRKANMIRSDFAGSEF
                     IKCDFRNARLENSDLSQMRFTACKFIEAKIESSLLMHSMLSNCDLTSSDFAKCNLERA
                     SLINCDLSRANFSGANLRYSDLTGISIRDTMFHNADLRGARVDNHWKHYLFQQRVLGY
                     NTIRWITPESTRTP"
     gene            2344..2724
                     /locus_tag="PLD82_06630"
     CDS             2344..2724
                     /locus_tag="PLD82_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65095.1"
                     /translation="MKSLAGLVFVMDDDDAVRNLVVLMLKRFGLEVESFACGEDLLVR
                     YNAGMPLPDLMLLDIQVGAGMGGEECLQKLHDLGSRIPAIAMTGMAGQGGSCPEACGF
                     SGYLGKPFTITDLHAVVDRQFNRE"
     gene            2729..>3513
                     /locus_tag="PLD82_06635"
     CDS             2729..>3513
                     /locus_tag="PLD82_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG65096.1"
                     /translation="MSEDVRSREDLLRENEMLRRWVSELGAVNTRERQIRSQGGDETC
                     LELYREILSLKTSLREVKERYHAMLGAMPDLLFIQNPDGTYIDYNAFSTVRYYVPPHE
                     FLGKKMHDILPKEVADNGLACVRTVLASGQARCIEYHLSNDGEDHYYEGRVVPCGSDR
                     VLTIVRDITERKRFEHCLREMATHDSLTELPNRLLFQDRLSHAIDRAKRSGNTVALLF
                     LDLDGFKEVNDTCGHAVGDQLLVLMARRLLDTVRDNDTVARLGG"
BASE COUNT          742 a          912 c         1073 g          786 t
ORIGIN      
        1 cgcgtcctgg gggagggatg atcgcggatt ccatcgaaac ccgtcccaac ttctcttcca
       61 gaccacgggg tggctggccg agggcgatcc tgatggcgtt cgccatcagg tcgacaccgc
      121 tcgagtaggg gtaggtgtag gctgacatga atccgcccga gagacgggca gcgatctctc
      181 cgaccatggc gccggtgggc gtgaccttga tgtctccctt ggcagcaccc atgtcgatcc
      241 cgagcgcttt gatgccggcg acaaagacac ggatcgcgtc ttcctgcatg gctggcggaa
      301 gattggatgg catgatgtgt ccggtttcga caaaccaggg cggacgggcg atcagcctgt
      361 ccgcaacccc ggtgatgtgg atttcacccc gatagacaag ggcatcaatc gaaagctcgg
      421 gcccttccat atattcttcg atgatgactt caccggaggg cgtcgcctcc ttggcaaagg
      481 cgaatgcgga ccggattccg gcttcatcgt cggccctgga gacaccgcgg gcacccatgt
      541 tgtcgcttgg tttgagaacg accgggcccc gtccgagacg gttgaaatgg tcgagggcgt
      601 catccagggt ccagcattca ccgaaccggg ggcagggaac ggcatgttca tgaagacgac
      661 tgcgcatctt caatttattt gaggcggctt ccgcgttgat gaagcggatt cccgggagcc
      721 cgagggcgtt ggcaaccgca gcaactgtca tggatgcgtc ggttcccacg gtcaggaccc
      781 cgtcgatatg ccggatctcc ctgtgatagc ggcgggcctc gcggaccgag ccgtcgatgt
      841 cccgcgtgct gatgatgatc cggtcatcgg agagggccag tccgggtgcg gaggtgctgt
      901 agtcggtggc gacagtgtgc atccccattt ccttggcttt cttgatcacc gggacctgca
      961 gtctgccggc gccgataatg aggactgtct gctcttcttg catgctcact gtcctttcaa
     1021 ggtgtgattc cgaacgatgt gttaaaaaag cctcccgtcc gtgactgacg ggaggcctgg
     1081 ggaccgcttg acagatcagg gtgtccgggt ggattccggc gtgatccagc ggatggtgtt
     1141 gtatccgagc acgcgttgct ggaaaaggta gtgcttccag tggttgtcga ctcgtgcacc
     1201 ccggagatcg gcattgtgga acatcgtgtc acggatggaa attcccgtca ggtccgagta
     1261 gcgcagattg gccccggaaa aattggcacg ggagagatcg cagtttatca gactggcacg
     1321 ctcgaggttg catttggcga aatccgatga ggtcagatcg cagttcgaga gcatggagtg
     1381 catgaggagt gagctctcga tcttggcctc gatgaatttg caggcggtaa aacgcatctg
     1441 gctgagatcc gaattctcca gtctggcgtt tctgaaatca catttgatga attcggatcc
     1501 ggcgaaatcg gaacggatca tattggcctt cctcaagtta gcctggtcaa agcgggcaaa
     1561 gttgagtttc acctgcgaaa gatcggagtc ggcaaagctg gtttggacga tccgggatcc
     1621 catcaaattc gctcccctga aatcgcaggc cgagaaaacg gcgttggaaa ggttggcaaa
     1681 ctggatattt gccagtcgga gatccgtatt tgtcaggctg gcaccggtca gattgacctg
     1741 ctggaggtcc gcccgctgga gattggcatc cacgaggcgg gcccgtgaga ggttgctccg
     1801 tcggagatcg acatacagga ggatagtccc gtcaagaaca attctttcaa gatcggaatc
     1861 attgagcttg gcttccgtga gattcgaaaa ccggatatcc gcattgcgga gaacggagcg
     1921 ggagagatcg gtataggcca tctccactcg ccagagtccg atattggtca ggactgctcc
     1981 ggagagatcg gtgttcgcaa agcgtgtatg cttgcgccag gtattccagt attccacacc
     2041 acgtttgaga ttgttgagat gggaagcctc ataaccagag agaattgtca cgagtgaaag
     2101 gcttgtgacg gcaacgagta tccaaatgga gcttcgtttc atgctgtctc cttgatgtca
     2161 ctccggtgga tccagtcaat ggacgcagga taccggtctt ctcccctact atcggtcagc
     2221 aaccctcaaa tctgcagtct catgtgaaac cgggagcgaa accggaatct cccgagcggt
     2281 cggaattttg tggaaatctc cacagctatt caggatcata tgactatggg gacttttcag
     2341 gaaatgaaat cgcttgcggg cctggttttt gtcatggatg atgacgatgc cgtcagaaat
     2401 ctcgtcgtcc tgatgctcaa acgtttcggt ctcgaggtcg aatcttttgc gtgcggggaa
     2461 gacctgcttg tcaggtacaa tgcgggaatg cccctcccgg atctcatgct gctggatatc
     2521 caggtcggag cgggcatggg cggagaggaa tgtctgcaaa aactgcatga tcttgggtcg
     2581 aggataccgg ccatcgccat gaccggaatg gccgggcagg gcggttcctg ccctgaagcc
     2641 tgcggtttca gcggttatct cggcaaaccc ttcaccataa cagacctgca tgctgtcgtt
     2701 gaccgccagt tcaatcggga gtgataccat gagtgaggat gtccgcagtc gcgaagactt
     2761 gctgcgcgag aatgagatgc tcaggcgctg ggtcagcgaa ttgggcgcgg tgaatacccg
     2821 agaacggcag atccggtcgc agggcgggga tgagacctgt cttgagcttt acagggagat
     2881 cctcagtctc aaaaccagtc tgcgcgaggt caaggaacgc taccatgcca tgcttggtgc
     2941 catgccggat cttcttttta tccagaaccc cgacggaacc tatatcgatt acaacgcgtt
     3001 ctctacagtc cgctattacg tgccaccgca tgaatttctg ggcaagaaga tgcacgatat
     3061 ccttcccaag gaggtcgcgg acaacggatt ggcctgtgtc cggacagttt tggcgagcgg
     3121 gcaagcacgg tgtatcgaat accatctttc caatgatggc gaagaccact attacgaggg
     3181 acgggtcgtc ccctgtggat cggaccgggt tttgacgatt gtccgggata tcaccgagcg
     3241 caagcggttc gagcactgtc tcagggagat ggcgacccat gactcgctga ccgaacttcc
     3301 caacaggctc ctgttccagg accggctttc ccatgccatt gatcgggcca aacgttcggg
     3361 gaacaccgta gccctgcttt ttctcgatct tgacggattc aaggaagtga atgatacctg
     3421 tgggcatgcc gtcggggatc agcttctggt gctgatggca agaaggcttc ttgatactgt
     3481 gcgggacaat gataccgtgg cccgtctcgg tgg
//
LOCUS       DAOSHE010000213         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24820_length_3510_cov_3.371925, whole genome shotgun sequence.
ACCESSION   DAOSHE010000213 DAOSHE010000000
VERSION     DAOSHE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24820_length_3510_cov_3.371925"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLD82_06640"
     CDS             <1..269
                     /locus_tag="PLD82_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65097.1"
                     /translation="NGGQMKMEDYLGRPQKEVQQLMTDNQTKGEELYNVGQYRESLPW
                     LDRGIFFSQVLIKQAELRKRAIDLLNEAQREVDEAKKRLDRSNG"
     gene            complement(266..1723)
                     /locus_tag="PLD82_06645"
     CDS             complement(266..1723)
                     /locus_tag="PLD82_06645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG65098.1"
                     /translation="MVVRYLAFFVLLTAALCFGACEEIHTVLAYRTPIFIEGTPEALP
                     VDAKTVISAAILTNSGWVPMQPYSELPEKFAVQTGIKDRVQVIMEHRGVLDVYPGSLV
                     VINDCTLSNVTVLQGKCAFMPLVKPLADAYNALKTDAERVEFIRQIKRRYLEEKNNLF
                     FVKNPFVIETTFSSQAAPKGKSFSIIVDSNHVLDFGDFKLDKLKPSFALIRSNAASGE
                     GYRYMAVTGLDIYENADKLHFTAAAKDVAGNWVKMRTYIPTEAWRASLLNGTSVKAGT
                     QPGAGQSDVFVRRYRERLNLKMQNPAFKRSYLASLEQQNRQIGFYWKSQQVLRQSALI
                     ASKERDFTRIFATVSPVKYWNGNFAVPATGVITSGFGHYRWYYGGFSSVHRAIDIANV
                     VGTSIHAPNAGKVVYVGNTPDRGNNIVIDHGLGVYTCLFHLAEMFVENGDVVRKGDVV
                     ASLGNTGLSSGPHVHWEMVVNGRRVNPLDWTSRKY"
     gene            complement(1815..2282)
                     /locus_tag="PLD82_06650"
     CDS             complement(1815..2282)
                     /locus_tag="PLD82_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65099.1"
                     /translation="MRIRLWIVLLGMLGMTGCTDIETVYASTVKGFGRFLEAVSAVHL
                     QPGLERPHLDMVTFLEGDGDTLLDGLDALARLRSQDSPRFWQLFEVNRAGIIAVFRKM
                     DGIDRRERLLLGAFTRVFAPREEPLEMVRYAWDRQMLETWITLLLRESRSVLR"
     gene            complement(2828..>3510)
                     /locus_tag="PLD82_06655"
     CDS             complement(2828..>3510)
                     /locus_tag="PLD82_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65100.1"
                     /translation="HLCPVCGVALPLVDGGTDWERRRELGLASAFVRTVGGVFRSPST
                     VFASLRGGVSHLGAVLLAVILGFFSSLIQAIWTMALVPGETKAAFAELLKNFTVEPIP
                     QAALDDIFRVIMLGQVALSPIFSILALYLEAGLIWLALSIARVQGLRFSMVLLLVALS
                     EISGLAVVIPQIGGMLAMVLGVVFLTTGLGVRFRLTTGKALACALAPPVVLMLFFVLG
                     TGALSAIG"
BASE COUNT          882 a         1018 c          928 g          682 t
ORIGIN      
        1 tcaatggcgg tcaaatgaaa atggaagact acctcggtcg accgcaaaag gaagtccagc
       61 agctgatgac ggacaaccag acaaagggtg aagaactcta caacgtcggc cagtatcggg
      121 aaagcctccc ctggctggat cgcggaatct ttttctccca ggtcctgatc aagcaggccg
      181 agcttcgcaa acgtgcgatc gaccttctca acgaggccca gagagaagtt gatgaagcca
      241 aaaagcgcct cgatcgcagc aatggctagt acttgcggct ggtccagtcc agaggattga
      301 ccctccggcc attgaccacc atctcccagt gaacgtgagg cccgctcgac agtcccgtat
      361 tgccgagcga ggctacaacg tcacccttgc ggactacgtc tccgttctct acaaacatct
      421 cggcaagatg gaagaggcag gtgtagacac ccagcccgtg gtcgatcaca atattgtttc
      481 ccctgtccgg cgtattcccg acatagacga ccttgcccgc attgggtgca tggatcgagg
      541 ttcccacaac gttggcaatg tcgattgccc tatggaccga ggaaaagcca ccatagtacc
      601 agcggtaatg cccgaatccg gaggtgatca ctccggttgc cgggacggca aagtttccgt
      661 tccagtactt gaccgggctg accgtcgcaa aaatccgtgt aaagtcccgc tccttgctgg
      721 caatcagggc gctctgtcgc aggacctgct gtgacttcca gtagaacccg atctgcctgt
      781 tctgctgctc caacgacgca agataggagc gcttgaacgc cgggttctgc atcttgaggt
      841 tgagccgttc ccggtagcgc cggacaaaaa catcactttg cccagccccc ggctgcgttc
      901 cggccttgac gctggtgcca ttcagcagcg aggctcgcca ggcctcggtc gggatatagg
      961 tccgcatctt gacccagttt cccgcaacat ccttggccgc agccgtaaaa tgcaacttgt
     1021 ccgcgttttc atagatatcc agaccggtca cggccatata gcgatagcct tcgcccgagg
     1081 ccgcattcga cctgatcagg gcaaaggacg gcttgagttt atccagcttg aaatctccaa
     1141 aatcgaggac atggttcgaa tcaacaatga tggaaaaact cttccccttg ggtgcggcct
     1201 gggacgaaaa cgtggtctcg atgacaaagg ggttcttgac aaagaacaag ttgttctttt
     1261 cttcgagata gcgccgcttg atctgccgga taaattccac ccgttcggca tcggtcttca
     1321 aggcgttgta cgcatccgcc aacggcttga ccagaggcat gaaggcgcat ttgccctgga
     1381 gaacggttac gttggaaagc gtgcaatcgt tgatgacaac cagactcccg ggatagacat
     1441 ccagcacacc ccggtgttcc atgatgacct gcaccctgtc cttgatgccg gtctgaacgg
     1501 cgaatttctc gggaagctcg ctgtagggtt gcatcggcac ccagccggaa ttggtcagga
     1561 tggcagcgct gatcaccgtc tttgcatcaa ccggcagggc ttccggtgtt ccttcaataa
     1621 aaatcggggt ccggtaggca agaaccgtat gaatctcctc acaggctccg aaacacaatg
     1681 ccgcagtcag caggacgaag aacgccagat atcgaacaac catgttctct ccctcaagat
     1741 agagactcat ccgccactgt gcaggcgcat tgttttctct atcggcgttt tcattccgtt
     1801 ccataaactg caggtcagcg aagcacactg cgcgactccc gcagaaggag cgtgatccag
     1861 gtctccagca tctgtctgtc ccacgcatag cggaccatct cgagcggctc ttccctcgga
     1921 gcaaagaccc gggtaaaggc acccaggaga agccgttccc tcctgtcaat cccgtccatc
     1981 tttctgaaga cggcgatgat cccggccctg ttgacctcaa aaagctgcca gaatcgcgga
     2041 gaatcctgac tccggagccg tgccagcgcg tccaggccat ccagcagtgt atccccgtca
     2101 ccctcgagga aggtgaccat gtcgaggtgc ggccgttcca gtccaggctg cagatgcacc
     2161 gcactcactg cttcaaggaa acgcccgaag cccttgacgg tggaagcgta gacagtctcg
     2221 atgtccgtgc agcctgtcat gcccagcatg cccaaaagca caatccacaa ccggatcctc
     2281 atgtccgccc cctgaaaaaa cgtcctgcaa gactactcaa aaaaagagac ccgagcaacg
     2341 cagcgggtct ctctagataa aaggaggtac attgagaaaa tctggtgttg tgctgccgaa
     2401 cctcaagggg agtcaaccgt tgcaaagcat gctgtctgac tggtgaaaag cagttgtgcg
     2461 acatgtttgg ataagctctg ttgtcctggt tgaaaagctg tgttactccc ctcttccgtt
     2521 caagcctggt gtaataccgt gttgcctgac gtgttgtctg accagctgca ctacaacccc
     2581 atttatagca attcccatgc cagctcgcaa atattttgag attttacaaa tttttgagga
     2641 aaaaacgcga attcgagcga tttttcagga attcaaccag cctatcgcag gaaaggagac
     2701 tgttcccagt ccaaaaaagc tgtgtgcatc ccaggaagca ccatggtaca gactcaaaaa
     2761 ttctcaaatt tgagaaaatg aattctcaaa aatgagaatt ctcaaatttg agagaaaagt
     2821 cccaggctca accgatggcc gaaagcgctc ccgtcccaag gacaaagaac agcatgagaa
     2881 caacgggcgg agccagtgca caggccaatg ccttgcccgt cgtcaaccgg aagcgaaccc
     2941 cgaggccggt tgtcaggaag acaacaccca gcaccatggc gagcatcccc ccaatctggg
     3001 gaatgacgac cgcaagcccg gaaatttcgg agagggcaac aagaagtagc accatcgaaa
     3061 accggagccc ctggacccgg gcgatcgaga gtgccaacca gatcaatccg gcttcaagat
     3121 agagggccag gatcgagaaa atgggcgaga gcgcgacttg gcccagcatg atcacacgga
     3181 aaatatcatc caacgcagcc tgcggtatgg gttccacggt gaagttcttg agaagttcgg
     3241 caaaggcagc cttggtctcg cccggaacga gggccatggt ccagatggcc tggatcagac
     3301 tgctgaagaa cccgagaatg accgccagga ggacagcgcc aagatgcgag acaccgccac
     3361 gtagagaggc aaacactgtt gacggtgacc ggaagacgcc cccgacagtc cgtacaaagg
     3421 cggaggccag accaagctcc cgccggcgct cccagtccgt gcccccgtca accaagggga
     3481 gggccacacc acagacgggg cagagatgct
//
LOCUS       DAOSHE010000214         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_24975_length_3493_cov_2.796102, whole genome shotgun sequence.
ACCESSION   DAOSHE010000214 DAOSHE010000000
VERSION     DAOSHE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24975_length_3493_cov_2.796102"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLD82_06660"
     CDS             <1..379
                     /locus_tag="PLD82_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG65101.1"
                     /translation="NPERVKEAVEVRRRFDRDALLARMAGDDEMYQRLLTEVPRVMPA
                     YIADLHTAIAGEDEALIRKRAHRIKGAALGMSFGILARLGQELEMLDPWDMSRARLLG
                     ADLEKEFSAILSLVQRESGNPSA"
     gene            419..2116
                     /locus_tag="PLD82_06665"
                     /pseudo
     CDS             419..2116
                     /locus_tag="PLD82_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
     gene            2437..>3493
                     /locus_tag="PLD82_06670"
     CDS             2437..>3493
                     /locus_tag="PLD82_06670"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOG65102.1"
                     /translation="MALSFYETLPFWSAFWNAMGYDIVTSGPTTRAIATVGIDRSTAE
                     TCFPVKVFHGHASSLLDAGVDYLFVPSVINMALDGSSSEHNFNCTLVQSAPFLLRSAF
                     RGREIPLLDPVVHMQYGYRRVIRELEPLAKSLGIPRQRLARALARAWEAQRLWREELA
                     REALAFVERHRDRPLAVIVSRPYNGCDPGLNLDIPRRLAENGIPALPLESLPLTHVAS
                     GGADALSRQDGDELWGNMYWRYGQRIMRGLEWIAGRPGTLPVYITNFSCGPDSFLLHY
                     AQETMAGRPLLVLELDEHSADAGIVTRLEAFLDSAVPRAWNEVSRVTPVQVKTQKTLR
                     GKKVWVPNMSDVAIPIAA"
BASE COUNT          633 a         1077 c         1085 g          698 t
ORIGIN      
        1 aaatccggaa agggtcaaag aagcagtcga ggtccggaga cgctttgacc gggatgctct
       61 cctggcacgg atggccgggg acgacgagat gtaccagcgc ctcctgaccg aggtcccgcg
      121 tgtcatgccc gcctacatcg ccgacctcca cacggctatc gccggggagg atgaagccct
      181 catccggaaa cgggcacaca ggatcaaggg ggcagccctg gggatgagtt ttggcatcct
      241 ggcacgcctc ggtcaggaac tggagatgct ggatccctgg gatatgtccc gggccagact
      301 gcttggagcc gatcttgaaa aggaattttc cgcgattctt tccctggtcc agcgcgaatc
      361 gggaaatcca tcggcataaa attttcacta tttttgattg atgaacacgc tctcagtcat
      421 gacatccccc gttctcggcg tcgatatcgg ttcggttgcc gtcaaggccg ccttggtatc
      481 agcaaccggg atcacccatc ccgtctatcg ccggattgac ggttccgcac tcgatgtttt
      541 ccgttccctc ctggcctcgt accgtgaaac cctgggcaac gccatgcccg ggcggctggc
      601 cctgaccggg cgtggtgtcg agagtctggc ggcggccggc ggatggcctg tcgtcaacga
      661 gattttcgcc caagggaccg gtgcactcgt gctggaaccg gcggcgcgga gcgtgatcga
      721 tatcggcgga aatgacgcgg cctggctggc gctctccgaa aacgccagag tggatgcctt
      781 ctccctggcc tcctttgcca cgaattcccg ctgcgccgcc gggaccggct cctttcttga
      841 ccaacaggct gcccggttgg gactctccat cgaagacgag ttctcccgga tggcactctc
      901 cgccgaaaac atcccccaca tcgccggacg ctgttcggtc ttcgccaagt cggacatgat
      961 ccatctccag cagatcgcga ctccggaccg cgagatcgtg gccggactgt gccaggcgat
     1021 ggccgaatcc ttccggagtg acatcatgaa gggccggatt cccgatctcc ccgtcgcgtt
     1081 ccaggggggc gtttcacgga accaggccat ggcccatttt ctgcgcaagg ttttccagct
     1141 cgaagaggaa cagctcctgg tgaacgagct gggacatctg gcaggagcga tcggagccgc
     1201 agtgcgcggg cattgtgacg ggaaatgcat tccccttgcg gatgcgctgg cgctcgcgag
     1261 cattccggtg attgaggagg gggggcttct cccgctcaag gatcctctgg aggcctatcc
     1321 cgcgcaggaa gagaaattcg tttttccgga agacggtgca acggtcgatg tgtttttggg
     1381 catcgacatc gggtcggtca gtaccaatct ggtactcctc gattcggttg gccgccttgt
     1441 gaccaaacgc tatctcccca cggccggtcg tcccattgat gcggtcctcg aaggattgaa
     1501 ggatatccag gaggaatgcg gagcccgggt gcgtgtcctg ggatgcggaa cgaccggctc
     1561 gggacgctat ctgatcgcgg atctcttcgg ggccgacatc gtcaaaaacg agatcaccgc
     1621 ccaggcccgc ggtgccctcc acctctgccc cgacaccgat acgatcttcg agatcggcgg
     1681 gcaggattcc aagtacgtgg cgatcgacaa cggggttgtc accgatttca cgatgaacaa
     1741 ggcctgcgct gccgggaccg gatcgttcat cgaggagcag gccaacctga tgggaatccc
     1801 gattcgccgg gtttttgccg aacgggcttt tgcctcatcg aggccagccc cgctcggaca
     1861 gcgctgcacc gtgttcatcg agagcgatgt cgtgcgccgg atggccgagg gtgacagtat
     1921 cgaaaatctc tcggccggaa ttgcctattc ggttgtcaag aattatctgc accgggtcgt
     1981 cgaaaacagg cggatcggga ctcgggtcct ttttcaggga ggaacagcct cgaaccatgc
     2041 agtcacggcg gccttccgga gcgtgacagg agtggatgtc cgggtgccgc cccacaatga
     2101 cgtgaccggt gccatcggga tggcggccat cgtgcgcgag gcctggatgg aggcgcgttt
     2161 cacgagtggc ttcatcggtc tggcctgtgg cgatctcgac tactcgatcc ggaccttcgt
     2221 ctgcaacgcc tgcgccaaca agtgccagat ccgggaggtg cgcagggagg gtggggtgcc
     2281 gttcttttac gggcatcgct gtgagcgcta cgagcactca cggaccaggg cgcccgacgc
     2341 ggaccagctt gattttttca aggaacgcac caggattctt gaacgctaca ccaacgccag
     2401 tccaccggcg gagtcccgcg gaaaatcggg atcccgatgg cactctcctt ttatgagacc
     2461 ctcccgttct ggtcggcctt ctggaatgcg atggggtacg atatcgtcac ctccggcccg
     2521 accacgcgag ccattgcgac cgtcgggatt gaccggagta ctgccgagac ctgtttcccg
     2581 gtcaaagtct tccacgggca tgccagttcc ctgctcgatg ccggtgtgga ctatctcttc
     2641 gtaccctccg tcatcaatat ggcactcgac ggttccagtt ccgaacacaa cttcaactgt
     2701 accctggtcc agtcggcgcc tttcctcctg cgctccgcct tcagggggcg cgagattccc
     2761 ctccttgacc cggtggtaca catgcagtac gggtaccggc gggtgattcg cgaacttgaa
     2821 ccgttggcaa aatccctcgg gataccgagg caacgtctgg cccgggcact ggcccgggca
     2881 tgggaggccc agcggctctg gcgggaggaa cttgccaggg aagccctcgc ctttgtcgag
     2941 cgccatcggg acaggccgct ggccgtgatc gtgagccggc cctataacgg atgcgacccg
     3001 gggctcaatc tcgacatccc ccgccggttg gccgaaaacg ggattcccgc cctgccgctg
     3061 gagtccctcc cgttgacgca tgttgcctcc ggcggcgcgg atgccttgtc gcgtcaggac
     3121 ggggatgaac tctggggaaa catgtactgg cgctacggcc agcgcatcat gcgcggtctt
     3181 gagtggatcg ccggccgtcc cggaaccctg ccggtctata tcacgaattt cagttgcggg
     3241 cccgactcct tcctcctgca ttacgcccag gagaccatgg ccggccgacc gcttcttgtc
     3301 ctggagctgg atgaacattc ggcggatgcc ggtatcgtga cccgactcga agcgtttttg
     3361 gacagtgcgg ttccgcgggc ctggaacgag gtctcacggg tgacaccggt ccaggtcaaa
     3421 acccaaaaga cccttcgtgg gaagaaggtc tgggtgccca atatgagcga tgtggccatc
     3481 cccatcgcgg cgg
//
LOCUS       DAOSHE010000215         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25269_length_3466_cov_4.420111, whole genome shotgun sequence.
ACCESSION   DAOSHE010000215 DAOSHE010000000
VERSION     DAOSHE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25269_length_3466_cov_4.420111"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..872)
                     /locus_tag="PLD82_06675"
     CDS             complement(84..872)
                     /locus_tag="PLD82_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65103.1"
                     /translation="MKTPVTFIGRILGRIPHLLLHGLVLPIVLLVLYLVNLAMLTVSP
                     VVSLAWLHEGAAGYLGLLPADVERLAAGSGAPEVQIPEGVLLVLGILAFLVLVWLAIF
                     VWIFIQGISRFWFSRVLKQTYRRTALIWRHILIVFFPLAWFVAAGLILGADGLREEIT
                     EVAAMTTISHLGVILFLFGSSEARDWKKSGAGEHAVGCISAWLLAPLLTVAAVHAAWR
                     SSDSVPWFLAWLGTTAVEVFFRWRAYKRYYTSIMRHVVEDEKTD"
     gene            1011..>3466
                     /locus_tag="PLD82_06680"
     CDS             1011..>3466
                     /locus_tag="PLD82_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOG65104.1"
                     /translation="MYCKRLQIVFLVLLLFVPTGCQSQSPKTETLDAAKLVQARTSRP
                     DALASSPQASRYRTGVPDPVAINVPASLENPVFSSPTAETLGAIVHHLTASTPDPYLK
                     TKRIHDWITLHIAYDNDAFLHGKEGSQDPMVFLKTKRTTCGGFARLFKMMADQAGLEA
                     LTVTGVSRNYIGLARNTAAGHAWNAVRIDGIWHIIDATADNRGGFENDEFGEQKTYRD
                     GSLFLSPAAKRISNLAHKPEEQFVTPALSVSEFLSRPLLYDSILRYGMEPLPASLSKI
                     RKIPEPVNGGRYLLNRDELLASGDTWTIDFTVPPDVDVDLLLEDQNKARHPLHTWTER
                     VGGVTRCHFRPPRAGTWQASIRARYSDKQWPLQTIYVFNIRAERGGAILGPAVRILDA
                     GQRLGIRPDKETAPTSEGGVSLEVRHPESHTLTCFLYDSANKLVSSRVVTSHFPGVKR
                     FFVSLPDDGTYWLGVWARDIRTPGQSTEQVLTIPLPDRKKSRDNALSPAGFWIGNQTP
                     LTNGFRMGGSIVTRPDASGTWEVTCTAPPGEFVTSRLLDASGKVLYGMSSDTRQDNRW
                     TSRFIASGPGRYTARLYRLLPGGSMSSIAQAVLEADRASPAYARAALMPLAQMERSGI
                     RVLSHSISAASGQHTLVLSHPAWSAITASLKNASGGSVQGGVLFDHGETSTRIYCAAP
                     TAGNWRLDVSAKTDRNASGSSMVLSAHLPAQPGKTVQPPPGSFLATARVFVERGLSLQ
                     HAGWNGKTWTIVLKNPRGADIFWYHFDKAKKLVRGGVNETQQNGKYTASCTPGPGSIG
                     RLYLREGGKTYVIGQVRLAD"
BASE COUNT          805 a         1060 c          914 g          687 t
ORIGIN      
        1 aacgcaggat cacccggcct ccatttctac actctcaacc gggtcgagcc agttctctca
       61 atcgtgcgaa aactcggact tcattaatct gtcttctcat cctcaacaac gtgccgcatg
      121 atggatgtgt aatatcgttt ataggctctc caacggaaaa agacctcgac tgcggtcgta
      181 ccgagccagg ccagaaacca gggaaccgag tccgacgacc tccatgcggc atggacagcg
      241 gccaccgtca acaagggggc caggagccag gcggagatac agccgaccgc atgctcccct
      301 gccccgctct ttttccagtc tctggcttca ctgctcccga acagaaagag aatcaccccg
      361 agatgcgaga tggttgtcat tgcagcaact tcggtgattt cttcccgtaa cccgtccgct
      421 ccaagaatca gtccggcggc aacaaaccat gccaagggga aaaagacgat caggatatgt
      481 cgccaaatga gcgccgtcct gcggtaggtc tgtttcagaa ccctggaaaa ccagaaccgg
      541 ctgataccct ggatgaagat ccagacaaaa attgccagcc agacgaggac aaggaatgcc
      601 agtatgccga gaaccaggag cacgccttcc gggatctgga cttccggcgc gcccgatccg
      661 gcagccaacc gctcgacatc cgctggaagc agtccgagat agcctgccgc tccttcatgc
      721 aaccaggcaa gactgacgac cggtgaaacg gtcagcatgg ctaaattgac taggtagagg
      781 accagcaaga cgatgggcag gaccagtccg tgcaggagga gatgggggat cctgcccaag
      841 attcggccaa tgaacgtcac gggtgttttc attactccac tatacgtcag gatttcaagc
      901 tcatgcaatg catttcaaca aattccgcct catgccggtt cgaagaaaaa gactgtcgat
      961 gacgcggttc aggtgtacaa tggtgtcact tcctgacaaa ggtatcaaca atgtactgta
     1021 aacggcttca gatcgttttt cttgtacttc tgctttttgt tccgacaggc tgtcaaagcc
     1081 agtctccaaa aacagagacc ctcgatgcag ccaaacttgt ccaggcacgg acatcacgcc
     1141 cggacgcgct cgcctccagc cctcaagctt cgcgttaccg gaccggtgtc cccgatcccg
     1201 tcgcgatcaa cgtcccggcc agtctcgaaa accctgtctt ttcctcacca acggccgaaa
     1261 cgctgggggc tattgtccac cacctgacag cttcgacacc ggacccctac ctcaagacaa
     1321 agcggattca cgactggatc acgctccata tcgcctatga caacgacgcg tttctccacg
     1381 ggaaggaagg aagccaggat cccatggttt ttttgaagac caaacggaca acctgcggcg
     1441 gattcgcacg gctcttcaaa atgatggcgg atcaggccgg acttgaggct ctcaccgtaa
     1501 ccggagtctc ccgcaattat atcggcctgg cccgaaacac cgctgccggc catgcctgga
     1561 atgccgtccg cattgacgga atctggcaca tcatcgatgc aacggccgac aaccgtggcg
     1621 gattcgagaa tgacgaattc ggggaacaaa agacataccg tgacggctcc ctgttcctct
     1681 ccccggccgc gaaacgtatc tccaatctgg cacacaagcc ggaggaacaa ttcgtcaccc
     1741 ccgcgctctc ggtttccgaa ttcttgtcac gcccgctgtt gtatgattcc atcctgcgat
     1801 acggaatgga accgcttccc gcttccctgt caaagattcg caagatacct gagccggtca
     1861 atggcggacg ctatcttctc aaccgggacg agcttctcgc cagcggtgat acctggacga
     1921 tcgattttac ggttccaccc gatgttgacg tcgatctctt gcttgaagac cagaacaagg
     1981 cacgccatcc cctccataca tggaccgagc gtgttggcgg agtgacccgt tgccatttca
     2041 gaccgcctcg tgccggaacc tggcaggcaa gcatccgggc acgctatagc gacaaacaat
     2101 ggcccctcca gaccatctat gtcttcaata tccgcgccga gcggggtggt gccatccttg
     2161 gcccggcagt ccggatactg gatgccggac aaagactggg aatacgccct gacaaggaaa
     2221 cggcgccgac aagtgaaggc ggagtgagtc tcgaagtccg tcatcccgaa agtcacacct
     2281 tgacgtgctt tctgtacgat tctgccaaca aacttgtttc aagccgggtt gtgaccagtc
     2341 attttcccgg tgtcaaacgc ttttttgtct cccttcccga cgacggaacc tattggcttg
     2401 gggtctgggc ccgggatatc cggacacccg gacagtcaac agaacaagtc ctgaccattc
     2461 ctcttccaga caggaaaaaa agtcgggaca acgctctttc acctgcaggg ttctggatcg
     2521 ggaaccagac gccactcacc aatggcttcc ggatgggggg ctccatcgtc acaagaccgg
     2581 atgcaagcgg aacctgggaa gtgacatgta cagcacctcc gggagaattc gttacctcaa
     2641 ggctcctgga tgcctccgga aaagtcttat acgggatgag ttcggataca cggcaggata
     2701 atcgctggac aagccgcttc atcgcctcgg gtcccggccg gtatacagct cgactctacc
     2761 ggcttcttcc cggcgggtcc atgtcatcca tcgcccaggc cgtcctcgag gctgatcggg
     2821 ccagcccggc atacgcccgt gccgctctga tgccactggc acagatggaa cgaagcggaa
     2881 tccgggtcct ctcacacagt atctctgcag catccggcca acacacgctg gttctttccc
     2941 acccggcctg gagcgccatc acggcttcgc tgaaaaatgc ctcgggagga tcggttcagg
     3001 gtggcgtgct gtttgatcat ggagaaacct cgacccggat ctactgtgca gccccgacgg
     3061 ccgggaactg gcgtctggat gtatcggcca aaaccgaccg gaatgcctcc ggctccagca
     3121 tggttctttc ggcccatctc ccggcacaac ccggaaaaac cgtacaaccg cctccaggga
     3181 gcttccttgc cactgcccgg gtgtttgtgg agcggggact ctccctccag cacgccggat
     3241 ggaacggaaa gacatggacg attgtcctga agaatccgcg tggcgccgat attttctggt
     3301 atcatttcga caaggcgaaa aaattggttc gtggcggtgt caacgaaaca cagcagaacg
     3361 ggaagtatac ggcgagctgc accccgggtc ccggttccat cgggcgactc tatctccgcg
     3421 aaggcgggaa gacttatgtc atcgggcaag tcaggctggc cgatga
//
LOCUS       DAOSHE010000216         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25641_length_3430_cov_4.826667, whole genome shotgun sequence.
ACCESSION   DAOSHE010000216 DAOSHE010000000
VERSION     DAOSHE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25641_length_3430_cov_4.826667"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLD82_06685"
     CDS             complement(<1..254)
                     /locus_tag="PLD82_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65105.1"
                     /translation="MNQFQRVVWLARRNFVRGTFSNLAAIFLVSINCLVFFMWYLHRF
                     SPNPAFSGQQSPMMGLSELLLVVLLAMNFFSLLVIFWLIN"
     gene            complement(349..1551)
                     /locus_tag="PLD82_06690"
     CDS             complement(349..1551)
                     /locus_tag="PLD82_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG65106.1"
                     /translation="MSEPLYTTGQLADRAFLLRTGEIEYWITDKDTVTLSGSNIIVGA
                     SELLIAINRKEAVPRHASLVLPGPATQLSPIPAAKLASYITNWTIGFSVAHHIAETIT
                     KLHPLLAEKMNRLAETERFSRDLARSFVETLQLLEESSGNRQFPWLVSIMDKGKVTEA
                     YNFGLSIRDVVEEKKIDVTSEKLAQYKQVYLKETVICKEGEKAEDMFILMEGKIEVRI
                     KNNPIDIISRKGTIIGEMGLILAQPRTATLRALEKTHVIRINAKDMASIFKNDPGTFF
                     NMISSLAFRERINCEKIREYNEKIEMAAQGRGEYTVAQVDGYAREYAALVTDIHSAMQ
                     AHPEMDWLGQVSELVQRKATSLLSQVGTLTGEVYTIQERARSEERVVTRTSNRPVRDE
                     SMPHIDWF"
     gene            complement(1677..2138)
                     /locus_tag="PLD82_06695"
     CDS             complement(1677..2138)
                     /locus_tag="PLD82_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65107.1"
                     /translation="MNPDSPTGDEVRLREVLQRLVRTASGLDQTLSLDKAHPGTSCGD
                     RLQIRCDADRNGLAIAWNGEGCSLMQAGAALLVEVLSGKEPHAARLIVDGALAMASGE
                     TASPVEGLGDEPALVLGAFSTRPNRRECLLFAWRAMRDWFDSNPEETSGAG"
     gene            complement(2135..3325)
                     /locus_tag="PLD82_06700"
     CDS             complement(2135..3325)
                     /locus_tag="PLD82_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG65108.1"
                     /translation="MSFPGIGAVRAGFPCLATPGRIWLDSAATSLKLKTVIETVSGYY
                     SGWCGTVGRSSHAAAEEASHAVQEVRRVMARYLGVEDPAGIIFVPGTTGGINLLARAW
                     NSGRIVTSRLEHHSNLLPWHDAARRNAAEIESVGLDSRGGIDMEALERALRRPAGLVA
                     LSMRSNVTGAVLDCRRAADLAHRAGAVLFVDAAQEAARGPMNLLNDGVDAASFSSHKM
                     HGPTGSGVLWVRPDLLERLDRVESGGGMALQVDDDEMVVRIGHARFEAGTPHIAGILG
                     MGSAARWLMAIGRETITEYEDGLGAYLHQELARLDGVRIFSCGGGILSCTITGWHPHD
                     AADQLDQRGFAVRAGTLCAQPCLRALGVDSLLRASLAIHSTCEECQAFADAVRDLILQ
                     GGFR"
BASE COUNT          715 a         1061 c          969 g          685 t
ORIGIN      
        1 ctgttgatca gccagaagat gaccaggagg gagaagaaat tcatggcaag gaggacaacg
       61 agcaggagct cggaaagtcc catcatcggg gactgctgcc ccgagaatgc cggattggga
      121 gaaaaccggt ggagatacca catgaaaaac accagacaat tgatggagac aagaaaaatg
      181 gctgcgagat tcgaaaatgt cccacgcaca aaattccgtc gcgccaacca gacgacgcgc
      241 tgaaactgat tcactggatg taccaaccct tccgcaatct gacagcccta aatatagtca
      301 tgaatcacct gtctgcggca gcgagtctcg cggagcacga agaacggatc aaaaccagtc
      361 aatatggggc atgctctcgt cccggacggg ccggttgctc gtgcgtgtca caacccgttc
      421 ctcgctccgc gcccgctcct ggatggtata aacctccccg gtcagtgtac cgacctggct
      481 gaggagcgag gtggctttgc gctggacaag ttcgctgacc tggcccaacc aatccatctc
      541 cggatgggcc tgcatcgccg agtggatgtc cgtcaccagt gcggcatact cacgggcata
      601 gccgtccacc tgggcgacgg tgtattcccc cctgccctgg gctgccatct cgattttttc
      661 gttgtactcg cggattttct cgcaattgat cctctcccgg aatgccagcg aggagatcat
      721 gttgaaaaag gtccccggat catttttgaa gatactggcc atgtctttgg cgttgatgcg
      781 gatgacatgg gttttctcga gagcccggag ggtggccgtt cggggctggg ccagaatcag
      841 gcccatctcc ccgatgatcg tacccttgcg gctgatgatg tcgatcggat tattcttgat
      901 ccgtacttcg attttccctt ccatcaggat gaacatgtct tccgcttttt cgccctcttt
      961 gcagatcacc gtctccttga gatagacctg tttatactgg gcgagctttt cggaagtcac
     1021 atcgatcttc ttttcttcga cgacatccct gatggacaaa ccgaaattgt aggcctcggt
     1081 gacctttccc ttgtccatga tgctgaccag ccaggggaac tgtctgtttc cggaagactc
     1141 ttcgaggagc tggagggttt cgacaaagga gcgtgccaga tcgcgggaaa accgctcggt
     1201 ctctgccagc ctgttcatct tttcggccag aagggggtgg agcttggtga tcgtctccgc
     1261 tatgtggtgc gccactgaaa acccgatggt ccaattggtg atatacgatg caagttttgc
     1321 agcgggaatc ggactcaact gggtcgccgg accgggcaga accaggctgg cgtggcgggg
     1381 aaccgcttcc ttccggttga tggcgatgag gagttcgctc gctccgacaa tgatattgct
     1441 cccggagagg gtcaccgtat ccttgtcggt tatccagtat tcgatttcac ccgttcgcaa
     1501 caaaaaggcc cggtcggcaa gctgtccagt cgtatacagg ggctctgaca cgttttctct
     1561 cctgcgggat ggattgtagt gtacaccagg cggtccatcc ctggcaagaa aaaacatgcc
     1621 accttgaaac ctttgcattt cccttcaacg gttttaacaa gacaggaaac agcggttcag
     1681 ccggccccgg aagtctcctc ggggttgctg tcgaaccagt cccgcatggc acgccacgca
     1741 aacaagaggc actccctccg gttcggtctt gttgaaaacg ccccgaggac cagtgccggc
     1801 tcgtcaccca gaccctcgac aggtgatgcg gtttccccgc ttgccatggc aagcgctccg
     1861 tcgacgatca gcctggccgc gtgaggttcc tttccggaaa gaacctccac caggagagcg
     1921 gcaccggcct gcatcaggga acaaccttcc ccgttccagg cgatggcgag cccgttccgg
     1981 tctgcatcgc aacgtatctg gagccggtct ccgcagcttg tgccgggatg cgccttgtcc
     2041 agagaaagcg tctggtccag tccgctggcg gtccggacca gacgctgtag cacttccctg
     2101 aggcggacct cgtcacctgt cgggctgtcc gggttcatcg aaaacctccc tgcaggatca
     2161 gatcacgaac cgcatcggca aaggcctgac actcctcgca ggtactgtgg atcgcgagac
     2221 tggcccggag cagggaatcc acacccaggg cccgcaggca gggttgagcg cagagtgtgc
     2281 cggcccggac ggcaaagccc ctttggtcaa gctgatccgc tgcgtcatgg ggatgccagc
     2341 ctgtgatggt gcaactgaga atcccccctc cgcaggagaa aatccgtaca ccatccagtc
     2401 gtgctagctc ttgatggaga tacgcgccca gaccatcctc gtattccgtg atcgtttcgc
     2461 ggccgatcgc catcagccag cgggccgcac tccccatccc gaggattccc gcaatatgcg
     2521 gtgttcccgc ttcaaaccgc gcatgaccga tccgcacgac catctcgtca tcatcaacct
     2581 ggagcgccat cccgccaccg ctctcgacac gatccagtcg ctccagaagg tcggggcgaa
     2641 cccagagcac accactcccc gtcggtccat gcatcttgtg cgaggaaaaa ctggcggcat
     2701 ccacgccgtc attgaggagg ttcatgggac cacgggctgc ctcctgggcc gcatcgacaa
     2761 aaagcacagc accggcccga tgcgccagat cggccgcacg gcggcaatcc agaactgctc
     2821 cggtcacatt ggaccgcatg gagagagcaa ccagtccggc cggtcgccgc aaggcccgtt
     2881 caagggcctc catatcgatc ccgccccgag agtccagacc gactgactcg atctccgcgg
     2941 cattgcgcct ggccgcatca tgccagggaa ggagattgct gtgatgctcg agcctgctgg
     3001 tcacaatccg cccgctgttc catgcgcggg caaggagatt gatccctccc gttgtgcccg
     3061 ggacaaaaat gattccggca ggatcctcaa cacccagata gcgggccatc acccgcctga
     3121 cctcctgaac ggcgtgggaa gcttcttcgg cggcagcgtg ggacgagcgg ccgacggttc
     3181 cgcaccaccc gctgtaatac ccgctcacgg tttcgatgac ggttttgagc ttgagcgagg
     3241 tggccgcact gtcgagccag atccgccccg gcgttgccag gcaggggaag ccggcccgga
     3301 ctgccccaat cccgggaaag ctcaccggat accgagcttc tttttgatgg tacgcagccg
     3361 gaacgcgctg acaagaatca cgaccagcat atcaatcgtc accagcatca gggccgcaga
     3421 cgagaaccgc
//
LOCUS       DAOSHE010000217         3427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25678_length_3427_cov_5.906287, whole genome shotgun sequence.
ACCESSION   DAOSHE010000217 DAOSHE010000000
VERSION     DAOSHE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25678_length_3427_cov_5.906287"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="PLD82_06705"
     CDS             complement(<1..1878)
                     /locus_tag="PLD82_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65109.1"
                     /translation="MRTRRVKRNYSTNAASIVVHDNPGFDTRTMALIMFARLFPQLNL
                     VSTVLDTFVSLLTPGEFETLVGKIRPYWEKIPNSKRMKANVEESELSAPDQHGTAVES
                     LIECGAIHQRTVRELVGQVLKEAVSNRSDPRTVYAKQLQELCEVYRIDQYELECLLFL
                     YTVEEQSDFSNLFDRLNMTGMLGTLGKAIGAGAGKARQLLSSRSRLVTMGLIDDARNS
                     LRHSSFPDLDAGVHELLSGLVETGLRERFVKRDTGKHYPLAGFQIPAMSLSIMRDLLA
                     LEAPAQILLYGRPGTGKTELARSLAAKCNKLVYLFACDEENLPRRGTQRSALIAAADR
                     VARDDGILIVDEADNLLQTVQQGFSFFGQSPKEDTGKEWINNFLDTTRTRIIWITNAT
                     RGIDESVKRRFGFSLKFGDFSRTQRLAMWRMQCREQKISLPQRELERLAAEYPVNAGT
                     IATALKGIATVDSNGTQARLERIGEILSRQLELQGLSRQQDRTSTCRGYDADALRLDT
                     DRKALETSLAGFAEALHTQPRGSMLSMNCLFWGPSGTGKTEYARHLAGLLGRELIFKR
                     ASDLFDPYVGMTEHKIREAFQSASQQDAILFIDEADSFLRTQDKASRSWELSFVNEFL
                     TN"
     gene            2004..3017
                     /locus_tag="PLD82_06710"
     CDS             2004..3017
                     /locus_tag="PLD82_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HOG65110.1"
                     /translation="MNTAAATRAVEIYNRLCDALHSDTRITKQSLARALNCSEKTIQR
                     DLETLGEHFPGVQVDYDERLKTFFLAQDGVTGDEFKKSDIKIRLQNHDLFILLMSTHI
                     LSQYRDSPAFVQMQESLKRIAAQIPGNIAVITHQALARSLTTLAKPLRPVDPAIFDIV
                     KQGIESKAVLAITYRAPDGKTTDRRIDPLYLDNMDGDLYTIAWCHLRKEVRIFALSRM
                     TIARKTATSFTPDDHPEIRHYLENRFGIFMNCQQREYTIRLRFTADQTPYVRERTWHP
                     KQKLVERATGEVDLSFPATHLFEVTRWVLSWGPGVRALAPPALVSAVRDQLTATLSQY
                     EGK"
     gene            3107..3325
                     /locus_tag="PLD82_06715"
     CDS             3107..3325
                     /locus_tag="PLD82_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG65111.1"
                     /translation="MKAELAAIIEKAPESGYWAFCPEIPGANGQGETAEEARENLREA
                     VELVILDRREDMLRGVSEDSIEEKICIG"
BASE COUNT          766 a          970 c          944 g          747 t
ORIGIN      
        1 atttgtcaaa aactcgttga cgaaggagag ctcccaactg cgggatgcct tgtcttgcgt
       61 gcgcaaaaag ctgtcggcct catcaataaa gaggatggca tcctgctgcg atgcgctctg
      121 aaaggcttcg cgtattttgt gctcggtcat gccaacatag gggtcaaaga gatcactggc
      181 ccgcttgaaa atgagctcgc gcccaagcag cccggccaga tggcgcgcgt attcggtctt
      241 tcccgtcccc gatgggcccc agaagagaca gttcatagag agcatcgaac ctcgaggttg
      301 tgtgtgtaag gcctcggcaa aacctgccaa cgaagtttcg agtgccttgc ggtccgtatc
      361 gagcctgagg gcgtccgcat cgtaccctcg acaggtcgat gtcctatcct gttgccgcga
      421 gagtccctgc aattcaagct gtcgcgagag aatctcgccg atgcgctcga gcctggcctg
      481 cgtcccgttg ctgtccaccg tcgcgatacc cttgagggcc gtggcaatcg tgcctgcatt
      541 gacagggtac tccgcagcca atcgttcaag ctcgcgttgg ggcagagaaa tcttctgttc
      601 gcggcactgc atgcgccaca tcgccagacg ctgagtccgt gaaaaatcac cgaacttgag
      661 actgaagcca aagcgccgct tgaccgattc gtcgataccg cgggtcgcat tggtgatcca
      721 gatgatacgg gtccgggtgg tatccaaaaa attgttgatc cattccttgc ccgtatcttc
      781 ctttggactc tggccaaaaa agctgaagcc ctgctggacg gtctgcaaaa gattgtcggc
      841 ttcgtcaacg atcaggattc cgtcatcccg ggcaacacgg tcggcggccg cgatcagggc
      901 cgagcgctgt gtgccgcgtc gtggcaggtt ttcttcatcg cacgcgaaca ggtagacaag
      961 cttgttgcac ttggccgcca gtgaccgtgc cagctcggtc ttgcccgtgc ccggtcgccc
     1021 gtagagcagg atctgcgcgg gtgcctccag tgcaagaaga tcccgcatga ttgagagtga
     1081 catcgcggga atctggaatc cggccaaagg atagtgcttg cctgtgtcgc gtttgacaaa
     1141 gcgctcgcgc aggccggtct cgaccagccc gctcaataat tcatggacgc ccgcatcgag
     1201 atcgggaaag ctggaatgtc ggagactgtt tcgtgcatcg tcgatcaagc ccatggtgac
     1261 aagccggctt cggctggaaa gcagttgccg tgccttgccg gctcccgcac caatagcctt
     1321 gcccaatgtt ccgagcatcc cggtcatatt cagcctgtca aagaggttgg aaaaatccga
     1381 ctgctcctcg actgtgtaga gaaagagcag gcactcgagt tcgtactggt cgatccggta
     1441 gacttcgcac agttcctgca attgctttgc atatactgtg cgcgggtcgc tgcggttgga
     1501 gacggcttct ttgagaacct ggcccacaag ttccctgact gtgcgctggt ggatggcacc
     1561 acactcgatc aggctctcga ctgcagtgcc atgttgatcg ggcgcagaca attcggactc
     1621 ttcgacattg gccttcatcc gcttgctgtt gggaattttt tcccagtagg gccggatttt
     1681 cccgacgaga gtttcaaatt ccccgggtgt gagcaaggag acaaaggtat ccaggaccgt
     1741 ggataccaga ttcagttggg ggaaaagacg ggcaaacatg atgagggcca ttgtcctggt
     1801 gtcgaatcct ggattgtcat ggacaacaat gctggctgca ttggtactgt aatttctttt
     1861 tactctgcgc gttctcatgc tcgatatcct ccgtcgttcc gatacggaca gggtacgtcc
     1921 ccaaccagga caaactatgt ccccctttgt gcttgcgttt tattgccggg ggaggtatcc
     1981 tgacgaagag tatggaggtc gtgatgaaca ctgctgccgc gacacgcgcc gtcgaaatct
     2041 acaacagact ctgtgatgcc ctgcatagtg atacccgcat caccaagcaa tccctggccc
     2101 gggccctgaa ctgttcggaa aaaacgatcc agcgtgatct cgagacactt ggagaacatt
     2161 tccccggcgt ccaggtggac tacgacgagc ggctcaagac gttttttctg gcccaggatg
     2221 gggtgaccgg agacgaattc aaaaagagtg acatcaaaat ccgcctgcaa aaccatgacc
     2281 tctttatcct cttgatgagc acccacatcc tctcgcagta ccgtgattcg ccggcctttg
     2341 tgcagatgca ggaatcgctc aaacggattg cggcacagat ccccggcaat atcgcggtga
     2401 tcacccacca ggcgctcgcg cgcagtctga ccacgctggc caaaccgctc agaccggtag
     2461 acccggcaat ctttgacatc gtcaagcagg ggatcgagtc aaaggccgtg cttgccatca
     2521 cctaccgcgc acccgacggg aagacgacag accgccggat cgacccgctc tatctcgaca
     2581 acatggacgg tgatttgtac acgatcgcct ggtgccactt gcgcaaggaa gtccggatct
     2641 ttgccctctc acggatgacg atcgccagga aaaccgcaac ctcatttacg ccggacgacc
     2701 accccgagat ccggcactat ctcgaaaacc gcttcggcat attcatgaac tgccagcagc
     2761 gtgagtacac gatccgcctg cgctttacag cggatcaaac cccctatgtg cgcgagcgca
     2821 cctggcatcc caaacaaaaa cttgttgaac gtgctaccgg tgaggtcgac ctgtcttttc
     2881 cggccacgca tctgtttgaa gtgacgcgct gggtcctctc ctggggcccc ggtgttaggg
     2941 ccctcgcccc gcccgctctc gtcagtgctg tccgtgatca gctcacggcg actttgtcac
     3001 agtacgaagg aaaatgaaaa cactccttcc tgagcctgtt ccgttttcga cttgatcaag
     3061 tttgcataca gcagcatact gtatataaga agccaggagg aaatccatga aggctgagtt
     3121 ggctgctatt atcgaaaaag cacctgaaag tgggtactgg gcattttgtc ctgaaatacc
     3181 cggcgccaat ggccaaggcg aaacagcaga agaggccaga gaaaatctcc gcgaggctgt
     3241 cgagctggtc attcttgaca gacgtgaaga catgctgcga ggtgtatctg aagactcaat
     3301 cgaagaaaag atctgtatcg gatgaaacgg agagatccca agcagcggct cagaatggcc
     3361 gggtgctatt tgaaacgtga aggtgcttct cattcactct gggtacaacc aaaatccggg
     3421 gctatag
//
LOCUS       DAOSHE010000218         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25709_length_3424_cov_4.363016, whole genome shotgun sequence.
ACCESSION   DAOSHE010000218 DAOSHE010000000
VERSION     DAOSHE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25709_length_3424_cov_4.363016"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1315
                     /locus_tag="PLD82_06720"
     CDS             35..1315
                     /locus_tag="PLD82_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65112.1"
                     /translation="MIKHLRLSNVGPAATMELEFGRRLNLLTGDNGLGKSFLLDIAWW
                     AMTRRWPAEVNCKLTAGKVALPTDKKMGAEIAFSFSAASKTMDDASSFDPRMQGWAVR
                     PGRPANPGLVFYALADGSFAVWDPARNYWRQGNDEGRERPPAYVFSPNEVWDGLQRED
                     GVWLCNGLIRDWAGWQKENGRAFESLTAVLDVLSPSADERIVPGALTRISLDDVRDMP
                     TLKMPYGQEVPVVHASSGMRRIISLAYFLVWCWEEHVKAKALLGEEPDSKTVFLVDEV
                     ESHLHPKWQRTVIPALMRVMNTLLSTSDVQLLAVTHSPLIMASVEPFFDRTQDAWFDF
                     DLIDSRVCLEQRQFFRHGDVRSWLVSEAFDMKSGYSLEAERVLEEASVALSDEKFSNA
                     DARALDERLRGVLSETDPFWMRWRFVAEKKGWLP"
     gene            1312..1911
                     /locus_tag="PLD82_06725"
     CDS             1312..1911
                     /locus_tag="PLD82_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65113.1"
                     /translation="MIHVAPQPEPASFDGNVRRRGLAWLDRNNIPLNRSLQKGTVLEP
                     YWRNCLDDLYSLYSGTCAYLAIFFERVTGAGSTDHFVAKSKRVDLAYEWANFRLACTR
                     MNSRKACHDDILDPFEIETGWFRLELVSGRIFPDPGALPALRDTIQATIDRLGLDDRD
                     HREMRARHYQEYREGWYPSEFLCRRSPFVWAEAVRQNLL"
     gene            complement(2317..2391)
                     /locus_tag="PLD82_06730"
     tRNA            complement(2317..2391)
                     /locus_tag="PLD82_06730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2354..2356),aa:Val,seq:tac)
     gene            complement(2460..>3424)
                     /locus_tag="PLD82_06735"
     CDS             complement(2460..>3424)
                     /locus_tag="PLD82_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65114.1"
                     /translation="NPSNYEVIFKIAKIFMDRNFMDQALAYFMKVVALNSKYAEAYFN
                     IGQILFQQKKENEALIHFNNAVKYDSQMHKANYFLGVINKGNNNYHQAVACLDDAQKD
                     ADFRQKARLAKGQCYFEIGDFSKAVVEFERALKDVSEEDAVSLAIRYSLAATYEQMRD
                     LPSAIEQWEAIASVKPGYNDVLEKLSTYQELRTDDRLKDFLTASNTGFEQLCRELVMA
                     MGFDIIKFASENGNHAVVLANEPESKWRNTKVSNKLIHIFRDSDAISEQIVRAIQEEM
                     KRVNANKAICISTSKFSPGAREFASARPIDLIDRTGLATILKKV"
BASE COUNT          697 a          857 c         1024 g          846 t
ORIGIN      
        1 caagccggaa gacggggcgg aggtttgctg agtcatgatc aagcatttga gactgtcaaa
       61 tgtcggcccg gcggcgacga tggaactgga attcggaaga cggctgaatc ttttgacggg
      121 cgataatggt cttggcaaga gctttttgct ggatatcgcc tggtgggcga tgacccgccg
      181 gtggcctgcc gaagtcaatt gcaaactgac tgccggcaag gtggctttgc cgaccgacaa
      241 gaagatgggt gcggaaatcg cattttcgtt ttctgccgct tcgaagacga tggacgatgc
      301 cagcagtttc gatccccgga tgcagggttg ggccgtacgg cccgggcgac cggccaaccc
      361 cgggctggtt ttttatgcac tggcggatgg cagctttgcc gtctgggatc ccgcccgtaa
      421 ctactggagg cagggcaatg acgaggggcg tgaacgtccg ccagcatatg tcttcagtcc
      481 aaacgaagtt tgggacgggt tgcagcgaga ggatggcgtt tggctgtgca acggactgat
      541 ccgtgattgg gctggctggc aaaaggaaaa cgggcgagcc tttgaatcat tgaccgcagt
      601 cctggacgtg ctttctccct ccgcggatga gcggattgtc cctggtgcat tgacccgcat
      661 cagtcttgat gacgttcggg acatgccgac cctcaagatg ccgtatgggc aggaggtacc
      721 ggtagtgcat gcgtcctccg ggatgcgtcg catcatatcg ttggcgtatt ttctggtatg
      781 gtgttgggag gagcacgtga aggccaaggc cttgctggga gaggagcccg actcaaagac
      841 ggtctttctc gtcgatgagg tcgaatcaca cttgcatccg aagtggcagc ggaccgtcat
      901 cccggccttg atgcgcgtga tgaatacatt gctgtcaacg tcggatgtcc aactgcttgc
      961 agtgacgcat tctccgctga tcatggcttc ggtggagcct tttttcgaca ggacacagga
     1021 tgcctggttt gatttcgatc tgatcgacag tcgtgtgtgt cttgagcaac ggcaattctt
     1081 tcgccatggg gatgttcgca gttggctggt cagcgaggct tttgacatga agtccggcta
     1141 ttcgcttgag gcggaacggg ttctcgaaga agcgtccgtg gctttgagcg atgaaaaatt
     1201 cagcaatgcc gacgccagag cgttggacga gcgcttgcga ggcgtccttt cggagacgga
     1261 tccattttgg atgcgatggc ggtttgtcgc ggaaaagaag gggtggctgc cgtgattcat
     1321 gtcgcgccgc aacctgaacc cgcatctttt gacgggaacg ttcgccggcg cgggctcgcc
     1381 tggcttgaca ggaacaatat tccgctcaat cgaagcctcc agaaaggcac ggttctcgaa
     1441 ccgtattggc ggaattgtct tgatgattta tattcgctct acagcgggac ctgtgcatat
     1501 ctggccattt ttttcgaaag agtgaccggc gctggctcaa ccgaccactt cgttgccaaa
     1561 tccaaacggg ttgatcttgc gtatgaatgg gccaattttc ggcttgcttg cactcggatg
     1621 aacagtcgca aggcttgcca cgacgatatc ctcgatcctt ttgaaattga aactggctgg
     1681 ttccgtcttg agcttgtcag tggtcgcata tttcccgatc cgggtgcatt gccggctctg
     1741 agggacacca ttcaggcgac aatcgatcga cttggtcttg atgatcgtga tcatcgggag
     1801 atgcgtgccc ggcattatca ggaataccgc gaaggttggt atccgagtga gtttctttgt
     1861 cggcgctcac cgttcgtttg ggctgaggct gttcggcaga atttgctctg acagcaagca
     1921 ttgccatttg ttgcgtcctt gccattggtg caggtcatgt gctgcgcggt gaggactcca
     1981 agctttgcat gaacatcagt gacaccccat gaaaaagcgt cactggtcaa ggtacaagca
     2041 gagaggggga atatttcgat aattcattgc aagacaggaa cttcgatgtg gcccatgcgc
     2101 attcgctgcg ctcatgtcgg acattaccct gcgggcaaag tccctgcaaa ggggcacttc
     2161 gccttgtcct cgcaaggaca ccctccgcac tggcgtgctt cgggcaaagt ccctgcaaag
     2221 gggcacttcg cagaaacatc ctgttttggc ccttgcgcat tcgctgcgct catgtcggac
     2281 atccttgtcc tcgcaaggac agaaacatcc tgtttctggg cgatgcagga tttgaacctg
     2341 cgaccccctg cttgtaaggc aggtgctcta aaccagctga gctaatcgcc cgaagtcgaa
     2401 aaaagatagc acgggagggt tgcgggggca agtcttcccg gatttttctc ccgcagggat
     2461 cagacttttt tgaggatggt cgccagcccg gtccggtcga tcaggtcaat cggtcgggcc
     2521 gaggcgaact cgcgggcgcc aggagagaac ttgcttgtag aaatgcaaat tgccttgttg
     2581 gcgttgaccc gcttcatctc ttcctggatg gcgcgcacga tctgctcgga gatggcgtcg
     2641 ctgtccctga agatgtggat cagcttgttg gagaccttgg tgttgcgcca tttgctctcg
     2701 ggctcgttgg ccagcacgac ggcatggttc ccgttttctg aagcaaattt gatgatgtca
     2761 aatcccatcg ccatcacgag ttcgcggcag agttgctcga aaccggtatt tgaggcggtc
     2821 aaaaagtcct tgagccggtc atcggttcgc agttcctgat aggtcgagag tttttcgagg
     2881 acatcattgt atcccggctt gacgctggcg atggcttccc actgttcgat agctgagggc
     2941 aggtcccgca tctgttcgta cgtggcggca agggagtagc gtatggcgag agagacagca
     3001 tcctcctcgg aaacgtcctt gagtgcccgc tcaaactcga ccacagcctt ggaaaaatcc
     3061 ccgatttcga aatagcattg tcccttggcc aggcgcgcct tttgacgaaa atccgcatcc
     3121 ttttgggcat catcaaggca ggcaacggcc tgatggtagt tgttgttccc cttgttgatg
     3181 acgccgagga aataattggc cttgtgcatc tgtgaatcgt acttgaccgc attgttgaaa
     3241 tggatcaggg cttcgttttc ctttttctgc tggaacagga tctggccaat gttgaagtag
     3301 gcctcggcgt atttcgaatt gagggccacg actttcatga agtaggccag ggcctgatcc
     3361 atgaaattcc ggtccatgaa gatcttggca atcttgaaaa tgacctcata gtttgaggga
     3421 ttca
//
LOCUS       DAOSHE010000219         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25886_length_3408_cov_3.992544, whole genome shotgun sequence.
ACCESSION   DAOSHE010000219 DAOSHE010000000
VERSION     DAOSHE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25886_length_3408_cov_3.992544"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLD82_06740"
     CDS             <1..1219
                     /locus_tag="PLD82_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65115.1"
                     /translation="GVMPPQARLGRFRTSSWYARIDGNGLEDSLTALYRNTVLPDAFP
                     QPGDVTNAMRAAGEWFLRHQNPEGSFSYRYEPFSGRIDASTYSMPRHAGSMLALYRIF
                     RHVPDVRFARAADRALEYVRQRLVAAPRGKAVAEDGTVKLGTVAITALALTERRVAGD
                     RREDPLLRDLLDFIAFMQKKDGSFCHMFFPPKNRDEVYHGQNFSGQALWALSRGMKIF
                     GGYTNEFVRGMRVQAEGFWDFFLSRYYMFPFTWEMQAIAEAEGVVDPGLLERWRGFAY
                     DCADSMIDFLYDSDWPFPDYRGGYGAPEWFVPLAHAAGFQCEGINGAYAIALRDDPER
                     AALYRHALRETARFVLSQQVTPAMAMHYADSLMPLGGFRAGPADHRQQIDFTQHIIAF
                     LITALEQGVFDGQ"
     gene            1209..2195
                     /locus_tag="PLD82_06745"
     CDS             1209..2195
                     /locus_tag="PLD82_06745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG65116.1"
                     /translation="MANNPDRPGLKTLWAYIKEIGRELKRPAAWFFIIIALVLATKSL
                     EVIRTHRGQPPLFGEIFQPVLRAIGGFVADPDAIDRYLRRIPAFIYQIVIPFSALWFW
                     FILTGWKKSGGHLVRALREGFSVAWNGFGLSLRCIRDWWKTLLLIFCVMLPFIYAFAT
                     TESFRRHYPYLQRLRFERTELAKIDKGISEQTRLIASSASGIQTRQDDNARVELDKLE
                     ARARSVFWGSLGLLLLFELLRMLYMVSWEFFFRGFMLQGLLRLRLGWHAVWIQMLPYV
                     MLHSTKPGIELYYTIPSGVLLGVLAWKTGSIWPGFLLHFIGAVVFDVFAMFA"
     gene            complement(2147..3163)
                     /locus_tag="PLD82_06750"
     CDS             complement(2147..3163)
                     /locus_tag="PLD82_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65117.1"
                     /translation="MRNILLFSDESASILAGLKRWTEREPRNPLWATLRKTVPARPLT
                     LLTSGADLPARLPCFPAIEGMAWSLRTNRFSMEIAGGLLFSTATNEGRGAYRLLRSNQ
                     YAPTTASLIPIDTDRLTSLLFDDLEELLSVLSPCFRAGTQPGSRLINDWAANELSVFI
                     RRGLRYVHIRVVDPARALKARHTPGQPPLVITNHAGWSIRRMEMPAFLEPLTRLFIPG
                     QNPVCLAQKGEHMLFGPSPAALLSLITAGSRTNLFQSQPKDAREILGKKCNFLLVDTH
                     GVIPLIPSGIQGILRDPHLRQGTVYANLKSADGMILVSLLVDNGRSRQANMANTSKTT
                     APMK"
     gene            3206..3361
                     /locus_tag="PLD82_06755"
     CDS             3206..3361
                     /locus_tag="PLD82_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65118.1"
                     /translation="MVDGDIQFSDGVFALGKGDGFIGIEDTAGRKNTGPDKERPPACI
                     GDAEDAA"
BASE COUNT          644 a          923 c         1064 g          777 t
ORIGIN      
        1 gggtgtcatg ccaccccagg cacggcttgg acgcttcagg acatcctcct ggtacgccag
       61 gattgacggg aatgggctgg aggactcgct gacggcactc taccgcaata ccgtcctgcc
      121 ggacgccttt ccgcagccag gggatgtgac aaatgccatg cgtgccgcgg gtgaatggtt
      181 cctccggcac cagaatcccg aaggttcgtt ctcgtaccgc tatgagccgt tctcaggaag
      241 gatcgatgcg tccacgtaca gcatgccgag acacgcaggc agcatgcttg ccctctaccg
      301 gatcttccgt catgtcccgg atgtgcgatt tgcccgggct gccgatcgtg ccctggaata
      361 tgtccggcag cgcctggttg cggcaccacg ggggaaggcc gttgccgagg acgggactgt
      421 caagttggga accgtcgcca tcaccgccct ggccttgacg gaacggaggg tggccggtga
      481 caggcgggag gatccgctcc tgcgggacct cctcgatttc attgcgttca tgcaaaaaaa
      541 agatggcagt ttctgtcaca tgtttttccc gccgaaaaac agggacgagg tgtaccatgg
      601 gcagaatttc agcgggcagg cgctctgggc cctgtcccgt ggaatgaaga tttttggtgg
      661 atataccaac gaatttgtcc gcgggatgcg tgtgcaggcg gagggtttct gggacttctt
      721 cctttcgaga tactacatgt ttccttttac ctgggagatg caggccattg ccgaggcgga
      781 gggcgtggtc gatccgggct tgctcgaacg ctggcggggg tttgcctacg actgcgccga
      841 ctcgatgatc gattttctgt atgattcgga ctggcctttc cccgattatc gtggcggata
      901 cggtgcgccc gagtggttcg ttcccctggc ccatgcggcg gggttccagt gtgagggcat
      961 caacggggcg tacgcgattg ccctgcggga tgaccccgag cgtgctgctc tctatcgcca
     1021 tgccctgcgg gaaacggcgc ggtttgtcct ctcacagcag gtgactccgg ccatggcgat
     1081 gcactatgcc gattccctca tgcccctggg cggattcagg gccggacctg ccgaccaccg
     1141 ccagcaaatc gatttcaccc agcatatcat cgcttttttg atcaccgctc tcgaacaagg
     1201 ggtctttgat ggccaataat ccggacaggc ccggattgaa gacactttgg gcctatatca
     1261 aggagattgg ccgggaactg aagcgaccag cggcctggtt cttcatcatc atcgccctgg
     1321 tcctcgcgac caagtctctc gaggtcattc ggacccatcg cggccaacca ccgcttttcg
     1381 gcgagatctt tcagcctgtc ttgcgggcga ttggcggatt tgtggccgac ccggatgcga
     1441 tcgaccgcta cctccggaga atccccgctt ttatctacca aatcgtcatc ccgttttcgg
     1501 ctctctggtt ttggttcatc ctgaccgggt ggaagaagtc gggcggccat cttgtccggg
     1561 ctctccggga ggggttttcc gtcgcatgga acggttttgg tctctccctg cggtgcatca
     1621 gggactggtg gaagactctc cttctcatct tttgcgtgat gcttcccttt atctatgctt
     1681 tcgcaacgac ggagagtttc cgtcgccact acccgtatct gcagcgtttg cggtttgaac
     1741 ggacggaact ggcaaaaatt gacaagggga tttcggagca gacccggttg atagcctcca
     1801 gtgcatcggg gatacagacc cggcaggacg acaacgcaag agtcgaattg gacaagctcg
     1861 aagcacgtgc ccgatctgtt ttttggggaa gccttgggct tctcctcctc tttgaactct
     1921 tgcggatgct ctacatggtt tcctgggagt tctttttccg cgggttcatg ctccaaggcc
     1981 tgcttcggtt gcgactgggc tggcatgctg tctggatcca gatgctgccg tacgtgatgt
     2041 tgcacagtac caagccgggg attgaactct attacacgat accgagcgga gtcctgctcg
     2101 gagtcctggc ctggaaaacg ggttccatct ggccgggttt tttgcttcac ttcatcgggg
     2161 ccgttgtctt tgacgtgttc gccatgtttg cctgacggga tctcccgtta tcaacaagga
     2221 gcgagacgag aatcattccg tcagcactct tgaggttggc gtagacggtt ccttgcctga
     2281 gatgcgggtc gcgtagaatc ccctggatgc ccgaggggat gaggggaatc accccgtgtg
     2341 tgtcaaccag aaggaagttg catttttttc cgaggatctc acgtgcgtct tttggctgag
     2401 actggaaaag attggttcgc gaccccgcag tgatcagcga gaggagtgcg gccggagagg
     2461 gtccgaagag catgtgctca cctttttgtg ccaggcagac cggattttga ccggggatga
     2521 agagtctggt cagtggttca agaaaggccg gcatttccat tcgacggata ctccagccgg
     2581 catggtttgt gatgaccagc gggggctggc cgggtgtatg gcgggctttg agtgcacggg
     2641 ccgggtcgac aacccggata tggacatagc gcagtcctct ccggatgaag acggagagtt
     2701 cgttggcggc ccagtcattg atcaggcgtg atccgggctg ggttcccgct cggaaacagg
     2761 gactgaggac cgagaggagt tcttcaagat cgtcgaaaag caggcttgtc aggcgatcgg
     2821 tatcaatcgg aatgagggaa gccgttgtcg gtgcgtattg gtttgaccgc aggaggcggt
     2881 aggcaccacg accttcgttc gtcgcggtgc tgaaaagcag acctcctgca atttccatcg
     2941 aaaaccggtt tgtccggaga ctccaggcca tgccttcaat cgccggaaaa caggggagcc
     3001 gtgcggggag atccgccccc gatgtgagca gtgtcaaggg acgggcaggg acggtcttgc
     3061 ggagtgttgc ccagagtgga ttgcgtggtt cccgctccgt ccagcgtttc agaccggcaa
     3121 ggatcgaggc actctcgtca gaaaacagca ggatgttacg cacgagtgcc agggacaggc
     3181 gattccgccg accgcggagt ccgatatggt agatggtgac atccagtttt ccgacggtgt
     3241 gttcgctttg ggaaaaggtg atggtttcat cgggattgaa gacactgcgg gccgcaagaa
     3301 taccggaccc gacaaagagc gcccgccagc ctgcatcggc gatgcagagg acgctgcctg
     3361 atgcaatccg taatcaggga aattatcgct cccggttttc gagaaaac
//
LOCUS       DAOSHE010000220         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25955_length_3401_cov_4.124925, whole genome shotgun sequence.
ACCESSION   DAOSHE010000220 DAOSHE010000000
VERSION     DAOSHE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25955_length_3401_cov_4.124925"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLD82_06760"
     CDS             <1..724
                     /locus_tag="PLD82_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65119.1"
                     /translation="LAAEQMQEMLHALDMMGESITGLEGLANHQHTIVGEMTAMRRDL
                     SAIGETIADFSNRIRLANDDLRLKAFNALIMTTHLGEHGRGFGVLAREVQTISGDTKA
                     FVENVTTTIEHIIRDSTSAGSSGLAMAEPPQCEQPDRILTRLREMVSGGVNDFLDGTA
                     ALSLAISTAISGSSVLESLAHDLESDCRSLSELLPDAPLVETHSLEEDIYTMDSERAI
                     HRDYFGETGPPSGASDGSVELF"
     gene            758..1048
                     /locus_tag="PLD82_06765"
     CDS             758..1048
                     /locus_tag="PLD82_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG65120.1"
                     /translation="MKSSESITLAGGDLTLYGVGETWKKIRSFLSNGTLRCLDISEVT
                     ECDTAGVQLLLVSISALNESGSRGAVQGSSRAVTEALERCGFPGAVLTGTTV"
     gene            1066..1431
                     /locus_tag="PLD82_06770"
     CDS             1066..1431
                     /locus_tag="PLD82_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65121.1"
                     /translation="MGKTIMTCDDSASIRQMVRFTLSQAGYDIVEAVDGQDALAKLGT
                     QQVHLLITDLNMPNMDGVTLIKSVRADGKLRFMPILMLTTESQESKKQEGRAAGATGW
                     IVKPFNPEQLLAVVRKVLG"
     gene            1463..>3401
                     /locus_tag="PLD82_06775"
     CDS             1463..>3401
                     /locus_tag="PLD82_06775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOG65122.1"
                     /translation="MATSENYVETYRQEVEEHLVVIEEAVLDLEDNPGNREALNRLFR
                     SMHTIKGSGAMFGFEEIAGFTHHVESVLDRVRAGEVTITPNLINLVLASRDLIRTMLV
                     TEGPLPAAEALQCEQVVAALQLLEAGDVVHVSAPAPSAAPPLQGKESTIWRIQFRPSK
                     ETFSSGLDPARLIDELRHLGNCTVTPDKGNLPDFGHFDPEICYLGWECLLETTASLDS
                     IKDVFIFVDEASEVSITQIHETLDPENTEKPPRLGDILVERGDVTPAAIQDVLSKQKR
                     IGELLVAEGIVSETEVEAALSEQKLIERHKAGTGVSSVRVPSAKLDSLINLVGELVIV
                     QARLTQVSGELGIPILERPVEEIEQLTAELRDCVFNIRMLPIGTSFSKFRRLVRDLSV
                     ELGKEIQLETAGENTELDKTVIEKLDDPLMHLVRNSIDHGIEKPDLREKQGKARIGTV
                     RLEAAQHGAHVVISISDDGAGLDRDAILSRAVERGLLERDIEYTDKEVFAQIFHPGFS
                     TAAKVTSVSGRGVGMDVVKREVEALRGSINISSERGKGSRITLSLPLTLAIIDGLMVV
                     VGDSHYVLPMAYVEECVEYGQDDMARTHGRNMIAVRGDLVPFVRLREFFSAPGDLPPA
                     EQIVIVRVEEFRVGIACDRIVG"
BASE COUNT          773 a          994 c          981 g          653 t
ORIGIN      
        1 gcttgccgcc gaacagatgc aggagatgct ccatgccctt gacatgatgg gagagagcat
       61 taccggcctt gaaggtctgg caaaccatca gcacaccatc gttggcgaaa tgacggccat
      121 gcgccgtgat ctctcggcca tcggagagac aatcgcggac ttctcgaacc ggatccgctt
      181 ggccaatgac gatctgcgac tcaaggcctt caacgccctg atcatgacaa cacacctggg
      241 cgaacacggc cgggggtttg gtgtcctggc gcgggaagtc cagaccatct cgggagacac
      301 aaaagcattt gtggaaaatg tcacgacaac gatcgagcac atcatcaggg actccacctc
      361 ggctggcagt tccggactgg ccatggcaga gccgccgcag tgcgagcagc ctgaccggat
      421 cctgacccgc ctcagggaga tggtctccgg tggcgtgaac gactttctgg atggaactgc
      481 ggccctctct ctagccatct cgacggcgat ctccggatca tcggttctcg agtcccttgc
      541 ccatgacctc gaatctgact gcaggagcct gtccgaactc ctgcccgatg caccccttgt
      601 cgaaacccac tcgctggaag aagacatata tacaatggac agcgagcggg ccatccacag
      661 ggactatttc ggtgaaaccg gtcccccgtc cggagcctct gacggaagtg tagaattgtt
      721 ctgacgtggt atacaataac ccggaggaat catggcaatg aagagcagtg agagcatcac
      781 cctggctgga ggagacttga ctctctatgg agtcggtgag acttggaaaa agatccgcag
      841 tttcctctcg aacgggaccc tgcgctgtct cgatatcagc gaagtcaccg agtgtgatac
      901 cgccggggtc cagctccttc tggtttcgat ctctgccctc aacgagagcg gatcacgggg
      961 tgctgtccag ggaagttccc gggctgttac cgaagccctc gagcgttgcg gttttcccgg
     1021 tgcagtgctg accggtacaa cagtctgaaa cggaggaatc ccgggatggg caaaacaatc
     1081 atgacatgcg acgactcggc aagcatccgc caaatggtcc gctttaccct gtcgcaggcc
     1141 ggctatgaca ttgtcgaggc cgtcgacggg caggatgcac tcgcaaaact gggcacgcaa
     1201 caagtccacc ttttgatcac ggacctgaac atgccgaata tggacggggt gaccctgatc
     1261 aaaagtgtcc gggcggacgg caaattgcgt ttcatgccga tcctcatgct gaccacggag
     1321 tcccaggaga gcaagaaaca ggaaggtcgg gctgccggcg cgaccggctg gatcgtcaaa
     1381 cccttcaacc ccgagcagct tctggcagtc gtccgcaaag tcctcggcta gactgccggc
     1441 agttcaaaga acgggaaaaa caatggcaac ctcagaaaac tacgttgaga cctaccgcca
     1501 ggaagtcgag gagcacctgg tcgtgatcga agaagccgtc ctcgatcttg aggacaatcc
     1561 cggaaaccgg gaagccctca acagactctt ccgctccatg cataccatca agggctccgg
     1621 agcgatgttc ggatttgaag agatcgctgg attcacccac cacgtcgaga gcgtccttga
     1681 ccgcgtccgt gccggggaag taacgatcac gccgaatctg atcaatcttg tccttgcatc
     1741 cagggatttg atccggacaa tgctcgtgac cgagggtccc cttcctgctg cggaggctct
     1801 tcagtgcgag caggtcgttg ccgccctcca gcttctcgaa gccggtgatg tcgtccacgt
     1861 aagcgcccct gccccatcgg cagcgccccc gttacagggc aaagagagta cgatctggcg
     1921 gatccagttc cgtccatcga aggaaacatt ctccagcgga ctggatccgg cgcgcttgat
     1981 cgacgaactc cgccatctgg ggaattgcac cgttacaccg gacaagggca acctccccga
     2041 cttcgggcat tttgatcccg aaatctgcta tctcggctgg gagtgtcttc tggagacgac
     2101 ggcctccctg gacagcatca aggatgtctt catcttcgtt gacgaagcga gcgaggtctc
     2161 catcacacag atccatgaaa ctctggatcc ggagaatacc gaaaaaccac cgcgtctggg
     2221 ggatattctg gtcgaacggg gcgatgtcac cccggccgcc atccaggacg ttctctccaa
     2281 acaaaaacgg atcggagaac tccttgtcgc cgaagggatt gtcagcgaaa ccgaagtgga
     2341 agccgcactg agcgagcaaa aactgattga gcgtcacaag gccggaaccg gcgtctcgag
     2401 cgtccgtgtc ccgtcagcca agctggacag tctgatcaat cttgtcggag agctggtgat
     2461 cgtccaggcc agactgaccc aggtttcggg tgaactcggc atcccgatcc tggaacgtcc
     2521 ggttgaagag atcgaacagc tgacagcgga gcttcgcgat tgcgttttca acatccggat
     2581 gcttccgatc ggaacatcct tttcgaagtt cagacgactg gtccgggatc tctccgtcga
     2641 actcggaaaa gagatccagc tcgaaacggc tggagaaaac acggaactcg acaagacagt
     2701 catcgagaag ctggacgatc cgctcatgca tctcgtccgg aacagcatcg accacgggat
     2761 cgagaagccg gatctgagag agaaacaggg gaaagcgcgg atcggaacgg ttcggctcga
     2821 agcggcccag catggcgccc atgtcgtcat ctcgatctcg gacgacggag ccggtcttga
     2881 ccgggatgcc atcctgtcac gggccgtcga gcgcggattg ctcgaacgcg acatcgagta
     2941 cactgacaaa gaagtctttg cccagatctt ccaccccggt ttttcgaccg cggccaaggt
     3001 cacgagtgtt tctgggcgtg gcgtgggaat ggatgtcgtc aaacgggagg ttgaagcgct
     3061 tcgtggatcg atcaacatct cgagcgaacg tggcaaggga agccggatca cactctccct
     3121 tccgctgaca ctcgccatca ttgacggact gatggtcgtg gtcggggact ctcattacgt
     3181 cctccccatg gcctacgtcg aagagtgtgt cgaatacggc caggatgaca tggctcgcac
     3241 ccatggacgg aacatgatcg ccgtcagggg agatctcgtc ccctttgtcc ggctcaggga
     3301 attcttctcg gcacccggag acctgcctcc ggccgaacag atcgtgatcg tccgggtcga
     3361 agaattccgt gtcggaattg cctgtgaccg gatcgtcgga a
//
LOCUS       DAOSHE010000221         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_25972_length_3400_cov_3.822123, whole genome shotgun sequence.
ACCESSION   DAOSHE010000221 DAOSHE010000000
VERSION     DAOSHE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25972_length_3400_cov_3.822123"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLD82_06780"
     CDS             complement(<1..501)
                     /locus_tag="PLD82_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOG65123.1"
                     /translation="MDGAISLAGGKVRALVGDITRVEVDAIVNAANSGLMGGGGVDGA
                     IHAAGGPVVTDACRTLRMTGYPDGLPVGSAVSTPAGRLPAKHIIHTVGPAWKGGSNGE
                     DDLLASAYLGCLVLAESLKCTSIAFPAISTGIYGFPAGRAAGIVRRVLEEFFSTGERS
                     LQDVRLI"
     gene            complement(513..2147)
                     /locus_tag="PLD82_06785"
     CDS             complement(513..2147)
                     /locus_tag="PLD82_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG65124.1"
                     /translation="MLVASDISLSFGKRTLFKDVNISFTPGNCYGLIGANGSGKSTFL
                     KILSGEIEPSSGTVSKDHRERIAVLRQDHFAFDDVPVLETVIRGHAVLWNLMRERDEI
                     YAKETFSDEDGLRAGEIEAEFAEMNGYEAETEAASLLMKLGIDEGLQSKLMRDLEDSQ
                     KVRVLLAQALFGNPDILLLDEPTNHLDMRSVMWLESFLANFQNTVIVVSHDRHFLDRV
                     CSHIADIDFGKIQIYSGNYSFWQQASQLAMQQKKDLNKKNADKIKELQEFVQRFSANA
                     SKSKQATSRKKLIEKLELEELPSSSRRFPYVAFSPEREVGNVILNVTGLSMKIEGEQV
                     LDKFDLTVNKGDRIAFVGNMTLAKTTLFRVLAGEIKPDAGEYHWGQTITLSYFPKENT
                     EYFNSDNNLVEWLRQYSTNQEEAFVRGFLGRMLFSGEEGLKKARVLSGGERVRCMLAR
                     MMLSGANAMILDEPTSHLDLEAISSLNTGLENFQGVLLFASHDHQLVESVANRIIEFT
                     PKGVIDRMMDFDEYIESEEIAALRNQMYGHAQDLML"
     gene            complement(2248..2832)
                     /locus_tag="PLD82_06790"
     CDS             complement(2248..2832)
                     /locus_tag="PLD82_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOG65125.1"
                     /translation="MKSVCVYCSSSSRVLPAFIEEAHALSRALSRAGLELVYGGADVG
                     LMGIVAAGVKDGGGRVTGVIPEKLARLGWKGCDELVVTPDMRSRKQVMEERSDGFVTL
                     PGGFGTLEEALEIITLKQLQYHAKPVVLVNTAGFYDPLAAMFERIYTDGFARREFSSL
                     YHLAHSSDEAVDYLMAYSPPPPIQKWGMERTAGG"
     gene            complement(2959..>3400)
                     /locus_tag="PLD82_06795"
     CDS             complement(2959..>3400)
                     /locus_tag="PLD82_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG65126.1"
                     /translation="LLFVLGLVSAMVSSADTCLLTAGSILGCELAGVTGLARVRIFVG
                     LTGIITTLLALFFRDIIGLMISSYAVYTAGIASPLVVALVAGKHDRQPRRSWFIAGMI
                     VGGLCGMASAIFAIPVLVVWGTASSAIMAGISLRRKGTEAAQEA"
BASE COUNT          778 a         1036 c          883 g          703 t
ORIGIN      
        1 tatgagccgg acatcctgga gactgcgttc cccggtcgaa aaaaattctt caagaacacg
       61 gcggacgatt ccggccgctc tgcccgccgg gaaaccatag atcccggtcg atatggccgg
      121 gaaggcaatg cttgtacact tgagagattc agccaggaca agacaaccga gataggccga
      181 tgccagaaga tcgtcctccc cgttggaccc gcccttccat gcaggaccga ccgtatggat
      241 gatatgcttt gcgggaagcc tgcctgcagg agtggaaaca gcgctcccca cgggaaggcc
      301 atcgggatac ccggtcatcc gcagggtccg acaggcgtca gtcacgaccg ggccacctgc
      361 cgcatggatc gcaccatcga caccaccacc acccatcaac ccggaattag ccgcattgac
      421 gatggcatcc acttcgaccc gcgtaatgtc tccgacaagt gcccgtactt tcccgccagc
      481 caggctgatc gccccgtcca tagctgtccc ctttagagca tcagatcctg ggcatggcca
      541 tacatctggt tgcgcaatgc cgcgatctct tcgctttcga tgtactcgtc aaaatccatc
      601 atccggtcaa tcacgccctt gggtgtgaac tcgatgatcc ggttggctac cgattcgaca
      661 agctggtggt catgggatgc aaaaagcagt acaccttgga aattctcgag tcctgtgttg
      721 agggaagaaa tcgcttcgag gtcaagatgc gaagtcggct cgtcaagaat catcgcgttg
      781 gcgcctgaga gcatcatccg ggccagcatg cagcggactc tttcaccccc ggaaaggaca
      841 cgggccttct tcaagccctc ctcgccggaa aagagcatcc gccccaggaa accgcggaca
      901 aaggcctctt cctgattggt cgagtattgc cggagccact ccacaagatt gttgtcactg
      961 ttgaaatact cggtattttc tttgggaaaa tagctgagag tgatcgtctg tccccaatga
     1021 tattcaccgg catccggttt gatctcgccc gcgagaacgc ggaagagggt tgttttggcc
     1081 agagtcatat tcccgacaaa cgcgattctg tcccctttgt tgacggtcag gtcgaacttg
     1141 tcgagtacct gctccccctc gatcttcatg gagagaccgg tgacattgag aatcacgttc
     1201 ccgacttcac gctccggaga gaaggccaca tagggaaaac gacgggaact ggacggaagc
     1261 tcctcgagtt ccagtttttc gatgagtttt ttccgcgagg ttgcctgctt cgatttggag
     1321 gcattggcac tgaaacgctg gacgaactcc tggagttcct tgatcttgtc cgcatttttc
     1381 ttgttcagat cctttttctg ttgcatcgcc aactggctgg cctgctgcca gaaggaataa
     1441 tttcccgaat agatctggat cttgccaaaa tcgatatcgg caatatggga acagaccctg
     1501 tcgagaaaat gccggtcgtg ggaaacaaca atcacggtgt tctggaaatt cgccaaaaac
     1561 gactccagcc acatcacgga ccgcatgtcc aggtggttgg tcggttcatc cagaagcaga
     1621 atgtccggat tcccgaacag cgcctgggcc agcaggacac ggactttttg cgagtcctcg
     1681 agatcgcgca tcagtttcga ctgcaggccc tcatcgatcc ccagtttcat cagcagggag
     1741 gccgcctcgg tttccgcctc gtagccgttc atctcggcaa actcggcctc tatctcgccg
     1801 gcccgcagcc cgtcctcgtc cgaaaaagtc tccttggcgt agatctcgtc acgttcgcgc
     1861 atcaggttcc agagaacagc atgcccgcga atgactgtct cgagaacggg aacatcatca
     1921 aaggcgaaat gatcctgccg gagtacggca attcgttccc tgtggtcttt ggagaccgtt
     1981 cccgacgacg gttcgatctc tcccgagagg atcttgagaa aggtcgactt tcccgacccg
     2041 ttcgctccga tcagaccgta gcaatttccg ggagtaaagg atatgttcac atccttgaac
     2101 agggtccgtt ttccaaaaga caagctgata tcactggcaa caagcattcg actgcacttc
     2161 cttgagagta atcgttccgg ggtacggcga aaaaaacggc ccgcctggtg caagcggaac
     2221 cgcctctcgc aaatatacgc aattctctca gcccccggct gtccgttcca tgccccactt
     2281 ctggatggga ggtggcggac tgtacgccat gaggtagtcg acagcctcgt ccgatgagtg
     2341 cgccaggtgg tacagggatg aaaactcgcg gcgggcaaac ccgtccgtgt agatccgctc
     2401 gaacatcgcg gccaagggat cataaaaacc ggcggtattg accagaacaa cgggtttggc
     2461 atgatactgc aactgcttga gggtgatgat ctcgagggct tcttcaagcg tcccgaaacc
     2521 gcccgggagg gtaacgaacc catccgaccg ttcttccatc acctgcttgc gactgcgcat
     2581 atccggagtc accaccagtt cgtcacaacc cttccagcca agccgggcga gcttttcggg
     2641 gatcaccccc gtgacccgtc cgccgccatc cttgacaccg gccgctacta tccccatcaa
     2701 gccaacgtcg gcaccgccat agaccagttc aagtccggcc cgggaaagtg cccgtgacag
     2761 agcatgcgcc tcttcgataa atgcaggcag gacccggctg cttgaggagc aatacacaca
     2821 tacgcttttc atccttcaag cctatacgct ctttcctcca cgcgcaagaa aatcttcagc
     2881 cccggcaccc tctccctacg ggcgaagtat acgcttgcga gtacttcgct cgtcaagagt
     2941 acccttcgcc cagcaccttt acgcttcttg cgcagcctcg gtgccctttc gcctgaggga
     3001 aatcccggcc atgatcgcgc ttgaggctgt tccccagaca accaataccg gtatggcaaa
     3061 aattgcggaa gccatgccac agagtccccc gacaatcatc ccggcgatga accatgaacg
     3121 ccggggctgt ctgtcgtgtt tccccgcaac caacgccact accaagggag atgctatccc
     3181 ggcggtatag accgcatatg aggaaatcat caagccgata atatccctga aaaagagcgc
     3241 gagcagggtg gtaatgatcc cggtcagacc cacaaagatc cggacccggg ccagtccggt
     3301 cacacccgcc agctcgcagc caagaatgct ccccgctgtc aggagacagg tatctgccga
     3361 tgaaaccatc gcggacacca gcccgaggac aaagagcagg
//
LOCUS       DAOSHE010000222         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_26487_length_3354_cov_3.911791, whole genome shotgun sequence.
ACCESSION   DAOSHE010000222 DAOSHE010000000
VERSION     DAOSHE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26487_length_3354_cov_3.911791"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /locus_tag="PLD82_06800"
     CDS             <1..2150
                     /locus_tag="PLD82_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOG65127.1"
                     /translation="SVQQEFSSEGMIAQLLDGYEPRPSQQEMAGHVARICNTGIHGVI
                     EAPTGTGKTLAYLVPLVSWAQANGKRVLVSTHTINLQHQLFDKDIPLVRKLLKDDFRA
                     VLVKGRSNYVCRRRVVEGLDGSGQELFPREDLAQLESLAGWIDGTKTGDRAELTGVPD
                     ELWDRVASDGDFCPGPRCPFMQECFFRQARRQAIEARILVSNHALFCTDLAMQREAGQ
                     SAGILPPVDRIVIDEAHNLEDVATDCFADSVSASSILRQLGRLSHRGAKPSGILHHLV
                     RQVEILGSDSDPFVQVLQEEIVPQVQNLHRLVEPCFESLSRVFSERTGSEAGHSFRIQ
                     QTDQAIRREITPHLERLSLPLAGLAKALQETGAQIQNLLEKLPEDKDEETADCVFACQ
                     QVVQFSARIDQYANLLADIPLLSRRDMVYWVEQRLQRGKLRLTFFRSPLSVREILAEV
                     LHSGNKGVIYTSATLANSTGRFDFFSNRIGLDLLPAARQEYRQLPSEFEYPDRVLLAV
                     NTGFPEKPEADFPVRFARFARDCIRGTRGRIFFLFTSWRMLNECWDATMEILPEEMAS
                     TCLKQGDTDRIHLLQTFKNLQHAVLFGTSSFWEGVDVKGDRLIAVVIVRLPFMVPDDP
                     LVSARMEEIEKSGRSSFFEYSLPHAVIRFRQGFGRLMRTRDDYGVVIVLDPRIVSKRY
                     GQTFFRALPETRYTDGDDGAIVLAVREHLARFE"
     gene            2147..>3354
                     /locus_tag="PLD82_06805"
     CDS             2147..>3354
                     /locus_tag="PLD82_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65128.1"
                     /translation="MSVRTGWIFLLLLFFLLPGFAGQAAGEERIRFEVRSGIRYLVRE
                     SAQKPEVGPLAGRGQLTPGGPAAVTGSDRAGVQPQAWEASKVWAYSGSATQGSLLITV
                     PVSIEHRFDTIHPDDEAESVRVRNGLREYLWTVPRNERPEYAPRPALAVWGTPSPPGD
                     VAAWLLRIYLDRKPSRLALGLTRTAGASRSVVLERAAWIHQSLVQGADSLDPALLPEE
                     TPHGITTTAGYACDFFSIGRALGWKVSLVIPLSSRYALPLGMPGIRIDDGTSVRWYFP
                     ASRVFTPGRVPWFLSGLQALCIAPDGKHETVQLSAVTEGETSYTVRVTADRSARLKAG
                     ISGEYTGTDAAIVRAGILRSGRQAFISGLTGNLPASTLAVRHILQLLSPVVLDCALAG
                     THELGVLEGVL"
BASE COUNT          648 a          937 c         1025 g          744 t
ORIGIN      
        1 aatccgtcca gcaggaattc tcgagcgagg ggatgatcgc ccagcttctt gacggctacg
       61 aaccacggcc gtcgcagcag gaaatggccg gccatgtcgc acgaatctgc aataccggga
      121 tccatggcgt gattgaagct ccgaccggaa ccgggaagac gctggcctat cttgtccccc
      181 ttgtttcctg ggcccaggcc aacgggaagc gagttctggt ttcaacacat acaatcaatc
      241 ttcagcatca gctctttgac aaggatattc ctctggtccg gaaactcctc aaagacgact
      301 tcagggccgt tctggtcaag ggtcggagca attatgtctg ccgccggagg gttgtcgaag
      361 ggctggacgg cagcgggcag gaactctttc cgcgcgagga tctggcccag ctggagtcat
      421 tggccggctg gattgatgga acgaaaaccg gtgacagggc tgaattgaca ggggtacccg
      481 acgagctttg ggaccgggtt gccagcgatg gagatttttg tcccgggccg cgctgtccct
      541 tcatgcagga gtgttttttt cgtcaagcac gacggcaggc aatcgaggcg agaatcctgg
      601 tctcgaatca tgcgctgttc tgtaccgatc ttgccatgca gcgcgaggcg gggcagagtg
      661 cgggaatcct tcctccggtt gaccggatcg tgattgacga ggcccataat ctcgaggatg
      721 tggcgacgga ctgttttgcc gattctgtct ccgcaagttc gatcctgaga cagcttggac
      781 ggctttccca caggggggcc aaaccctccg ggattctgca ccatcttgtg cgccaggtgg
      841 agattctggg gagcgattcg gatccctttg tgcaggtgct gcaagaagaa attgttcccc
      901 aggtgcaaaa cctgcacagg ctggtcgaac cctgtttcga gagcctctcc agggtgttca
      961 gtgagcggac cggatcagaa gcggggcaca gtttccgcat acaacaaacg gatcaagcca
     1021 tccggcggga gatcacaccg catctcgaaa ggctctctct tcccctggcc ggattggcca
     1081 aggcactcca ggagaccgga gcgcaaatac agaatcttct ggaaaaattg cctgaagaca
     1141 aggacgagga gacagcggac tgtgtctttg cctgccagca ggtggtccag ttctcggccc
     1201 ggatcgatca gtatgccaat cttctggccg atataccgct tctttcccgc cgcgacatgg
     1261 tgtactgggt cgagcaacgc ctgcagcgtg gaaaactccg gttgaccttt ttccgctcgc
     1321 cactctcggt ccgcgagatt ctggcggaag tgctccattc ggggaacaag ggtgtgatct
     1381 atacatccgc gacgctggca aacagtaccg gccggtttga tttcttctcc aaccggatcg
     1441 gtctggatct cctgccggct gcccgccagg aataccggca gttgccgtcc gaattcgagt
     1501 atccggaccg ggtgcttctg gccgtgaaca ccggatttcc cgaaaagccg gaggcagatt
     1561 tccccgtcag gtttgcccgt tttgccaggg actgcatccg gggaacgcgt ggccggatct
     1621 tttttctctt cacatcctgg cgcatgctca atgaatgctg ggacgcgacg atggaaatcc
     1681 ttcccgaaga aatggccagt acctgcctga aacagggcga tacggaccgg attcatctcc
     1741 tgcagacgtt caagaatctc cagcatgcgg ttcttttcgg aacgagctcc ttctgggagg
     1801 gtgtggatgt caaaggcgac agattgatcg ccgtcgtgat agtgcgcttg cccttcatgg
     1861 taccggatga cccgctcgtg agtgcgcgga tggaagagat cgaaaaatca ggccggtctt
     1921 cgtttttcga gtactcgctt ccccacgcgg tcatccgttt tcgacaggga ttcggccgtt
     1981 tgatgcggac ccgggatgac tatggcgtgg tgattgttct cgatccacga atcgtcagca
     2041 agcgttatgg gcagacgttt ttccgggctc ttcccgaaac ccgatatact gacggagatg
     2101 atggagcgat tgtcctcgct gtccgtgagc atctggcgag gtttgaatga gcgtgcggac
     2161 cggatggata ttcctgctgc tcctgttttt tctcctgccg gggtttgccg gacaggcagc
     2221 aggggaggag cgtatccggt ttgaagtccg gtcagggatc cggtatctcg tgcgggagtc
     2281 ggcacagaaa ccggaggtcg ggcctctcgc cggccgtggc cagctgacac ctggtggtcc
     2341 ggcggccgtg acaggttcgg acagggcagg ggtgcagcct caggcgtggg aagcaagcaa
     2401 ggtctgggcc tatagcggtt cggcgacaca aggttccctg ctgatcacag tacccgtctc
     2461 gatcgaacac cgcttcgaca ccatccatcc ggatgatgaa gccgagagtg tccgtgtccg
     2521 caatggtctg cgcgaatatc tctggactgt gccacggaac gaaagaccgg agtatgcacc
     2581 acgacctgct cttgctgttt ggggaacacc atcacctccc ggggatgtgg cagcctggct
     2641 cctccggatc tatttggaca gaaagccctc ccgcctggcc ctggggttga ccagaaccgc
     2701 cggtgcaagc cggtcagtcg tccttgagcg tgctgcctgg atccaccaga gtctggtgca
     2761 gggcgctgac tcgctcgatc cggcgctgct tccggaagag accccgcatg gcattacaac
     2821 gacagcgggg tacgcctgcg actttttctc gattggaagg gccctgggat ggaaggtgag
     2881 tcttgtgatc cccctttctt cacgatacgc tcttcctctc gggatgccgg ggatccggat
     2941 tgatgacgga acctctgtcc gctggtattt tcccgcttca cgggtcttta ctccgggcag
     3001 ggtcccctgg ttcctttccg gcctgcaagc cttgtgcatt gccccagacg gaaaacatga
     3061 gacggtccag ttgtccgctg ttacggaagg ggaaacgagc tatacggtca gggtgacggc
     3121 agacaggagt gcccgcctga aagccggcat ttcgggagag tatacgggaa cggatgccgc
     3181 cattgttcgt gccgggattc tccggtcagg aaggcaggcc tttatatcgg gcctgaccgg
     3241 caatctcccc gcatctacac tggcggtccg ccatattttg caattgctct ctccggttgt
     3301 cctggattgt gcacttgcgg ggacccatga actcggagtg ctggaaggtg ttct
//
LOCUS       DAOSHE010000223         3350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_26537_length_3350_cov_4.106222, whole genome shotgun sequence.
ACCESSION   DAOSHE010000223 DAOSHE010000000
VERSION     DAOSHE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26537_length_3350_cov_4.106222"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLD82_06810"
     CDS             <1..794
                     /locus_tag="PLD82_06810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG65129.1"
                     /translation="ASSWIKARTKHAPVVGLILGSGLGAVGDIIEHAEYFPYKDIPGF
                     PVSTVEGHAGRLVVGKLEGKDVVAMQGRFHFYEGYSMAEVTFPVRVMKRLGVKILVVT
                     NAAGAVNKGYTPGDLVLITDHINFQGTNALIGHNLDSFGPRFPDMSTPYDSELGRLAK
                     ETAFSLGFELREGVYAAMSGPSYETPAEVRMVRTLGGDAVGMSTVPEVVVANHCGMRV
                     LGISCMTNMAAGVLDQPLNHEEVIETSNQVRDRFIKLMLGIVKNA"
     gene            complement(1155..1880)
                     /locus_tag="PLD82_06815"
     CDS             complement(1155..1880)
                     /locus_tag="PLD82_06815"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG65130.1"
                     /translation="MKQQISLRLDRILSNLGYATRSTARALLSSGRVSIQGKPATNGA
                     CKARPEEVLLDGQPLDHPAGIFIMLHKPAGYVCSHEAGEGALIYDLLPDHWRRRTPVP
                     TSIGRLDKDTTGIVLVTDMMPLVHQLASPKHDIEKLYHVSLDPVGPALEDRLVEKFAA
                     GTLRLDPKDEKPCLPARLVLTGIHTADLTLSEGRFHQVKRMFAACGYTVVALHRSRFG
                     AYDLGDIPEGQWRDCPLPDGRIT"
     gene            2182..2400
                     /locus_tag="PLD82_06820"
     CDS             2182..2400
                     /locus_tag="PLD82_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017011991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HOG65131.1"
                     /translation="MEDRVNGEAILRRLLEMKMPFGKYAGRPIMDLPEDYLVWFSKKG
                     FPDGELGGLMALALEIRINGLEGMLGRR"
     gene            complement(2486..>3350)
                     /locus_tag="PLD82_06825"
     CDS             complement(2486..>3350)
                     /locus_tag="PLD82_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65132.1"
                     /translation="WRCSACSGVKADKELETAIKDAAANANLDMKYAFVRGPQEKIIS
                     DMILKKGVVASLPTLSLALISEDPKVRVVANRYLYREIKDYISEFEKNPEATPKAAIK
                     NLIKGIEMSKDYVTFYAVASTTMLATMYGMEDGLIKAVKAHPEKALKIEMVQNIMRYG
                     RLRTFPVVKEWAASSEKYALTHALSALRNMYRWTDEEKKTIGEWARQYLTVEDPETRV
                     RVIYALYQAGGSYYDTALEKVEREIEGGSVPKLFIDWLKNMAGNETDAQKVRRLSIIT
                     NGSKVAADPKK"
BASE COUNT          645 a          858 c         1029 g          818 t
ORIGIN      
        1 aagcctcgtc ttggatcaag gcccggacca agcatgcccc cgttgtcggg ttgattcttg
       61 gatccggtct gggtgcggtc ggggatatca tcgaacacgc cgaatatttc ccctacaaag
      121 acattcccgg gttcccggtt tcaaccgtag aaggccatgc cggccgactg gtggtaggga
      181 aacttgaggg caaggatgtt gttgccatgc agggccgttt ccatttttac gaagggtaca
      241 gcatggccga ggtgaccttc ccggtccgtg tcatgaagcg gctcggtgtc aagattcttg
      301 ttgtgacgaa cgccgccggt gcggtgaaca aaggctatac gcccggggat cttgttctca
      361 tcacggacca tatcaatttc cagggaacca atgccttgat cgggcataat cttgattcct
      421 tcgggccccg ttttccggat atgtcgacac cctatgacag cgagcttggc cgccttgcca
      481 aggaaacggc attttcattg ggcttcgagt tgcgagaggg tgtctatgcg gccatgtccg
      541 gtccgagtta tgaaacaccg gccgaggttc gcatggtgcg gaccctgggc ggggatgccg
      601 tcgggatgtc cactgttccc gaggttgttg tcgccaacca ctgcgggatg cgggttctcg
      661 gaatttcctg catgacgaac atggccgccg gagttcttga ccagccgctc aatcacgaag
      721 aggtgatcga gacatccaac caggttcgtg accgttttat caagttgatg ctcggtattg
      781 tgaagaacgc ctgaagcatt ccgttgatcc ggccttccag agggaccctc cgtcaaggat
      841 ggtccctctt tttttctgga tgaagatgga caccgggttg ttcttccatg gaacaccatg
      901 agtatgcgct ggccgttttc atcatgcaga tccggatgct tgcattctgt tgtatgtttt
      961 ttatgctcta catatatgta gaaacgcatt tatgtattgc tggtgatgct gtcgcgggag
     1021 gttgtcccat ggaacaggaa aaaaatcaga cacagcttcg ggatcggctc acttcttgaa
     1081 gggcaggagc tccgataatc cgggtacgga tgttgcagaa acaggcgcga ttgtctgaag
     1141 ctgtggcagg ggcatcaggt tatgcgcccg tcagggagcg gacagtcccg ccactggccc
     1201 tcggggatgt cgcccaaatc atatgctcca aaacgggagc ggtggagtgc gacgaccgtg
     1261 tagccacagg cggcgaacat ccgcttgacc tggtggaatc gtccttcgga gagggtcagg
     1321 tctgccgtat ggatgcctgt caggacaagc cgggccggga gacagggctt ttcgtctttc
     1381 gggtcaaggc gcagagttcc ggcggcgaat ttttcaacca gcctgtcttc gagcgccggt
     1441 ccgaccggat cgaggctgac atgatagagc ttttcaatat catgttttgg tgaagcaagt
     1501 tgatggacga gcggcatcat gtccgtgacc agcacgatcc ctgtggtatc cttgtccagc
     1561 cggccgatac tggttgggac gggtgtgcgc cgtcgccagt ggtccggcag gagatcgtag
     1621 atcagggcgc cttcgccggc ctcgtgcgag cagacgtacc cggccggttt atggagcatg
     1681 atgaagatac ccgccggatg atcgaggggc tgtccgtcga gcaggacttc ctcggggcgg
     1741 gccttgcagg cgccattggt tgcaggtttt ccctggatgc tgacccggcc gctggagaga
     1801 agggcacggg cggtactgcg ggtggcatag ccgaggttgg agagaatccg gtcaagtcga
     1861 agagagatct gttgtttcat cgaaaaaaca tagtacccgg aggacgcgga gtatattcct
     1921 ttatcgggac gggcagcaga acgtgtcggt attttttgat gaacacactc cggcctgatc
     1981 aggttgcatc gtgcgcgctg gagtttgttt gccggagcct tgccggcctg gcatggatcg
     2041 tgcggtccgt ttttgcgact cgtcggatcg agactggcct gaggatgttc atcaggaaaa
     2101 cctggatgtt gcgcaggcaa ttggctggcg atctgaatgt gccggtccgg gacaaggcag
     2161 cctgctgaca aggagagggg aatggaagac agggtgaatg gggaagccat cctccggcgc
     2221 ttgttggaga tgaagatgcc gttcggaaaa tatgcgggca gacccatcat ggatctgccc
     2281 gaagactatc ttgtctggtt ttcaaaaaaa ggctttcccg atggcgagct gggcggcctg
     2341 atggccctgg ctctcgagat caggatcaac gggcttgagg ggatgctcgg ccggcgttga
     2401 caatccttcg gcaaggaccg ggcctcccgt caaaacaccc ttcgttccga cgggagatgc
     2461 tggccggtcc cggctcttca aacggtcatt tttttggatc agccgcgact tttgacccgt
     2521 tggtgatgat cgagaggcgg cgcactttct gggcatccgt ctcgtttccg gccatgtttt
     2581 tgagccaatc aataaagagt ttcggaaccg atccaccttc gatttcccgt tcgacttttt
     2641 cgagggcggt gtcgtaatag ctgccaccgg cctgatacag ggcatatatc acacggaccc
     2701 tggtttctgg atcttcaacc gtcaggtatt gtctggccca ttcgccgatc gtcttttttt
     2761 cttcgtccgt ccagcgatac atgttcctca gggcggagag cgcatgggtc agggcgtatt
     2821 tttcactgct tgcggcccat tccttgacca cggggaaggt gcgcagccga ccatagcgca
     2881 tgatgttttg gaccatctcg atcttcaggg ctttttcggg atgggccttg acggccttga
     2941 tcagtccgtc ctccatgccg tacattgtgg ccagcatggt ggttgacgct acggcgtaaa
     3001 acgtgacgta gtccttgctc atctcgatcc ctttgatcag gtttttgatg gcagcttttg
     3061 gcgtggcttc gggatttttt tcaaactcgg agatgtagtc cttgatctcg cggtaaaggt
     3121 agcggttggc aacaacccgg accttggggt cttcgctgat cagtgccagg ctcaaggtgg
     3181 ggagcgaagc gacaaccccc tttttcagga tcatgtccga gatgatcttt tcctgtgggc
     3241 cgcggacgaa ggcgtacttc atgtccaggt tcgcattcgc tgcagcgtcc ttgatggctg
     3301 tttcaagctc cttgtcggct ttgacgccag aacaggccga gcatcgccag
//
LOCUS       DAOSHE010000224         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_26566_length_3347_cov_5.630316, whole genome shotgun sequence.
ACCESSION   DAOSHE010000224 DAOSHE010000000
VERSION     DAOSHE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26566_length_3347_cov_5.630316"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLD82_06830"
     CDS             complement(<1..1013)
                     /locus_tag="PLD82_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOG65133.1"
                     /translation="MDDEMDYLPLLPLREGVLLPGGATRIYVGRPQSLEALRKAGEGN
                     MRIFCIAQRDGTVENPTDPADFAEYGTLAKIDSIRNGTRGIRIVILSGLQGGRLVAMD
                     PLSGLVQVEPVREIADLDPETIHKSISALRSFKPQGDTLEPGLKAKMERGDFGSILQR
                     IVEHFAVVPIIAAELVREPSQKARLEKLVTILEIQRDTGSIRDEMHARVKQRIRDTQR
                     EYVLRELLRDVKGELGADGVHEEDDFASTLAQVPLPREARQRVDRELKRLEVVPPMSP
                     EYGVIRGYLEWLRDLPWSQTSKEEMDIRKVRKSLDASHYGLEDVKRGILEFVAARWLA
                     GEKG"
     gene            complement(1023..2357)
                     /gene="clpX"
                     /locus_tag="PLD82_06835"
     CDS             complement(1023..2357)
                     /gene="clpX"
                     /locus_tag="PLD82_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG65134.1"
                     /translation="MATKRTQNTGGNHEDMACSFCGKTQSEVNRLIAGQRVFICDRCI
                     AYCNQLLGEQPASLRDVQAEVVLPKPQEIKKVLDDYVVGQDTAKKVLSVAVYNHYKRI
                     NFNALSRDNRNDIELEKSNILLIGPTGSGKTLLARILARTLNVPFAIADATTLTEAGY
                     VGEDVENILLRLIQNAESLLKEQGNTHPTEKDVIGYAQRGIIYLDEIDKIARKSENVS
                     ITRDVSGEGVQQALLKIVEGTRASVPPQGGRKHPHQELLQIETKDILFICGGAFVGLE
                     NIIGKRIGQSVIGFNGDSGSKQASRDIFGLLSQATPDDLTRFGLIPEFVGRIPVVSAL
                     APLDEDDLVHILVNTKNSIVAQYRKSFEIEGVDLNFEDTAIRAIASLAITRNTGARGL
                     RAIIEDIMLDLMYEIPGMENVSGCVVTEGVVRKHEKPTLIYASGKKSAEKPA"
     gene            complement(2372..3016)
                     /gene="clpP"
                     /locus_tag="PLD82_06840"
     CDS             complement(2372..3016)
                     /gene="clpP"
                     /locus_tag="PLD82_06840"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG65135.1"
                     /translation="MSEEAQGMIEQMNLVPMVIEQSGRGERAYDIFSRLLKDRIVFIG
                     DPIHDVTANLAIAQMLFLVAEDPEKDISLYINSPGGSVTAGLAIYDTMQFIKCDVSTI
                     CIGQAASMAAVLLAAGTPGKRFALPNSRILLHQPMGGAGGQASEVDIQAREILRIREV
                     LNGILVRHTGKDLDTIRQDTDRDFFMAAEEAKAYGVIDSVIVKKPVESRPTDGI"
     gene            complement(3013..>3347)
                     /locus_tag="PLD82_06845"
     CDS             complement(3013..>3347)
                     /locus_tag="PLD82_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65136.1"
                     /translation="AYLEKTGKTVEKMREDFLPSAEKAAVTYLLVREIAKTENVKAED
                     ADIDEALDSYARYYGHDRDALRKHFIENGEIDSLVWRIVRRKTMDVLEKAATVSIEKT
                     VPFSEIKE"
BASE COUNT          644 a          945 c          948 g          810 t
ORIGIN      
        1 cctttttctc cggccagcca gcgggctgcg acaaattcca ggattccccg tttgacatcc
       61 tcgagtccgt aatggctcgc atcaagactc ttgcggactt tgcggatatc catttcttct
      121 tttgaagtct ggctccaggg gaggtccctc agccactcaa gataaccgcg gatcacccca
      181 tactccggag acatgggcgg aacaacctca aggcgtttca attcgcgatc aactctctgt
      241 ctggcttccc tcgggagggg gacctgtgcc agggtcgaag cgaaatcgtc ttcctcatgg
      301 acgccgtcgg caccgagctc tcccttgacg tcccggagaa gctcgcggag gacatattcg
      361 cgctgggtat cacggatcct ctgtttgacc cgggcatgca tctcgtcccg gatcgaaccg
      421 gtatcccgct gaatctcgag aatcgtgacc agcttttcga gacgggcctt ttgggaaggc
      481 tccctgacca gttcggcggc gatgatcggg acgacggcaa agtgttccac gatccgctgg
      541 aggatgctcc cgaaatcacc ccgttccatc tttgctttca gaccaggctc gagggtatcg
      601 ccctgtggtt taaagctgcg caaggcggag atgctcttgt ggatggtttc gggatcaaga
      661 tcggcgatct ctcggaccgg ttcgacctgt accagtccgc tcagcggatc catcgcgaca
      721 agtcgtccac cctgcagtcc cgagaggatc acgatccgga tcccgcgggt tccgttcctg
      781 atggaatcga tttttgcgag ggtgccgtat tcggcaaagt cggcaggatc ggtcgggttt
      841 tcgacggttc catcccgctg ggcgatgcag aagatccgca tatttccttc gccggccttg
      901 cgcagagcct caagagactg cggtctgccc acgtagatcc gtgttgcgcc accgggcaaa
      961 agtaccccct ccctcaaggg aaggaggggc aggtaatcca tctcgtcatc catgctgagt
     1021 cctcaggcag gtttctccgc actctttttc ccggatgcgt agatcagagt cggcttttca
     1081 tgtttgcgca cgacaccttc ggtcacaaca catccgctga cattctccat gcccgggatc
     1141 tcgtacatca aatcgagcat gatgtcttca atgatggccc gcagtccacg ggcgccggtg
     1201 ttccgggtga tcgccaggct ggcgatggcg cgaatcgcgg tgtcttcaaa attgagatcg
     1261 actccctcga tttcgaatga tttgcgatat tgggcgacaa tcgaattttt tgtattgacg
     1321 aggatgtgca caagatcatc ctcgtccaga ggagcaagcg cactgaccac ggggatccgg
     1381 ccgacaaatt ctgggatcaa tccgaagcgg gtcaggtcat ccggggtcgc ctgggacagg
     1441 agtccgaaga tgtcccgtga ggcctgcttg cttccactgt ctccgttgaa cccgatcacc
     1501 gattggccga tgcgttttcc gatgatgttt tccagtccga caaaggcgcc accacaaatg
     1561 aagaggatgt ccttggtttc gatctggagc agttcctgat ggggatgctt gcggcctccc
     1621 tgaggcggga ccgatgcgcg cgttccttcc acgatcttga ggagtgcctg ctggacacct
     1681 tcgccagaga catcgcgggt gatcgagaca ttttcgctct ttcgggcgat tttgtcgatt
     1741 tcatcgagat agatgatccc gcgctgtgca tatccgatga catccttttc ggtcggatgg
     1801 gtattccctt gttccttcag cagcgattcc gcattctgga tcaagcgcag gaggatgttt
     1861 tccacgtcct cgccgacata gccggcctcg gtcagggtgg tggcatctgc aatggcgaag
     1921 ggaacgttga gggtgcgcgc gaggattctg gcaagaaggg tttttccgct cccggttggt
     1981 ccgatcagga ggatattgct cttttcaagc tcgatatcgt tccggttgtc ccgggagagg
     2041 gcgttgaaat tgattctctt gtagtggttg tagacggcca ccgaaagaac ctttttggcg
     2101 gtgtcctgtc cgacgacgta atcgtcgagg acttttttga tctcctgggg tttggggagc
     2161 acaacctcgg cttgcacatc acggagggag gccggttgtt caccaagaag ctggttgcag
     2221 tacgcaatgc agcgatcaca gatgaagaca cgctgtcctg ctatcagacg attgacctcg
     2281 ctctgtgttt ttccgcaaaa cgagcaggcc atatcctcgt ggttgcctcc ggtattctgt
     2341 gtgcgcttgg ttgccattgc tcctccgatc ctcaaatccc gtccgtcggc ctggattcaa
     2401 cgggtttttt gacgatgaca ctgtcaatca caccgtatgc cttggcttcc tcggctgcca
     2461 tgaaaaaatc cctgtcggta tcctggcgga tcgtatccag atccttgcct gtatgccgga
     2521 cgaggatccc gttcagtact tctcggatcc gcaaaatctc ccgggcctgg atgtcgacct
     2581 cgctggcctg gccaccggca ccacccatgg gctggtgcaa gaggatacgg gagttgggca
     2641 gggcaaaacg ctttccggga gtccctgccg ccagcagaac ggcagccatg ctggccgcct
     2701 gtccgataca gatcgtcgaa acgtcacatt tgatgaactg catggtatcg tagatggcca
     2761 gacctgcggt cacggagcca cccggggagt tgatgtagag cgagatgtct ttttcgggat
     2821 cctcggccac gagaaaaagc atctgggcga tcgccagatt ggcggtgaca tcatggatcg
     2881 ggtcaccgat aaacacgatc ctgtccttga ggagtcgtga aaaaatatcg taggcccgtt
     2941 ctccacggcc cgactgctcg atgaccatgg ggacaagatt catctgctcg atcattccct
     3001 gtgcttcctc gctcattcct taatttccga aaacggtacg gtcttctcga tgctgacggt
     3061 ggcggccttc tcgaggacat ccatcgtctt gcgacggacg atgcgccaga ccagtgaatc
     3121 gatctcgccg ttctcgatga aatgcttgcg aagggcatcc cggtcatgac cgtagtagcg
     3181 ggcgtagctg tcaagggcct cgtcaatatc ggcatcctcg gctttgacgt tttctgtttt
     3241 ggcaatctcg cgcacgagca gataggtgac cgcggctttc tcggccgacg gaaggaagtc
     3301 ttcacgcatc ttctcgacgg tcttgccggt tttttccaga tacgcat
//
LOCUS       DAOSHE010000225         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_26915_length_3317_cov_4.023912, whole genome shotgun sequence.
ACCESSION   DAOSHE010000225 DAOSHE010000000
VERSION     DAOSHE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26915_length_3317_cov_4.023912"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1180
                     /locus_tag="PLD82_06850"
     CDS             <1..>1180
                     /locus_tag="PLD82_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65137.1"
                     /translation="AQEFDVCLVDYQLPDGTGTLLIREINKNPLAPPCIMLTSQEGYQ
                     TDLEAMQAGAVDYLTKDRLEPETLERSIRYALKNRRVEAALRESQERYALVVRGANDG
                     VWDWNPQSGRLYLSTRWKDILGYRDEEFEESMEHWFSRVHPDDLFEARTSLDDYLSSR
                     LRQLSNEYRMRHKDGHWVWVRVKGQAVFDAEGKAVRVAGSMGDITERRQALETLRIRE
                     QERELRLQRMEEELRMARTIQKQFLPRIVPAGPGFRFSAIYQPAEMVGGDLYDFYMEE
                     TACIGFICDVSGHGVPSALISGLVKATFEDAASMDGDPGSILTRMNGLLHDKMGTYFL
                     TAACFRYDQSTRDVTLASAGHNPLLLVRDGRVEELVAHGRVVGPFPEYVYATETWRLE
                     P"
     assembly_gap    1181..1280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1281..1558
                     /locus_tag="PLD82_06855"
     CDS             <1281..1558
                     /locus_tag="PLD82_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65138.1"
                     /translation="VGPFPEYVYATETWRLEPGDRILAYTDGINECVRFSPVEEMYGD
                     ERLMDFLAEKTSLDGEQFLQVLLDTLGTFSGNAPFEDDVTAIVLDIE"
     gene            1628..2044
                     /locus_tag="PLD82_06860"
     CDS             1628..2044
                     /locus_tag="PLD82_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG65139.1"
                     /translation="MKSYRKELWFEAKQRRELFNITGQVEDCLGESGIREGLALVNAM
                     HITASVFINDDETGLHHDFEVWLEGLAPEKPYNRYRHNGYEDNGDAHLKRTIMGREVV
                     VAVTNGKLDLGPWEQIFYGEFDGMRRKRVLVKIIGE"
     gene            2120..2851
                     /locus_tag="PLD82_06865"
     CDS             2120..2851
                     /locus_tag="PLD82_06865"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG65140.1"
                     /translation="MKKMLRMTGVVVSLVAMILPLTAYQSSQAELARRKSAVDRYRAA
                     GVFSEKVYQAVMSTPREYFMPEGQNHMTYEEVPVAIGWGQTITNQWFVAYMTHLLDVQ
                     ASDKVLEIGSGSGYQASILWQITKKVYTIEIVSQLAAIAKQRWQNLGYSSIVGKAADG
                     YYGWAEYGPFDKIVVTCAADHVPVYLMQQLKPGGVMVIPVGNPFDRQTLLKITKNMDG
                     TVRTERLQSVKFVPFTGKMLEKYGK"
     gene            2859..>3317
                     /locus_tag="PLD82_06870"
     CDS             2859..>3317
                     /locus_tag="PLD82_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65141.1"
                     /translation="MEKRGLVSVFLVSMAVLGAELGVIRYMSVASWSNFAGLIISVAL
                     LGFGLSGTILTVMRERIERRLQDWLFWSAAAFVVTSGYVLQFARAIPFIPQSLHQDGT
                     QVLYLALYYFLFFLPFFCGALFIKLSFIRMKTGINRMYLYNLMGSGVGALV"
BASE COUNT          675 a          846 c          964 g          732 t
ORIGIN      
        1 tggcgcagga gtttgatgtc tgtcttgtcg attaccagtt gcccgacggg accggaactt
       61 tgctgatccg cgagatcaac aagaacccgc ttgctccacc ctgcatcatg ctcacttccc
      121 aggagggcta tcagaccgac cttgaggcca tgcaggccgg ggcggtggat tatctgacca
      181 aagaccggct cgaaccggag accctcgaac gctcgatccg ctacgcgctt aaaaaccgca
      241 gggtagaggc ggcgctccgc gaaagccagg aacggtacgc cctggtcgtt cggggtgcca
      301 acgatggtgt ctgggactgg aatccccagt ccggacgact ctacctctcg acccgctgga
      361 aggacatcct cgggtaccgg gacgaggagt tcgaggagag catggagcac tggttctccc
      421 gcgtccatcc cgatgatctc tttgaggccc ggacctcatt ggatgattat ctctcctcac
      481 ggcttcggca gctttccaat gaatatagaa tgcggcacaa ggacggccac tgggtctggg
      541 tgcgtgtcaa gggacaggct gtctttgacg cggagggaaa agcggtccgg gtggccggct
      601 cgatgggtga catcaccgaa cgccggcagg cgctcgagac cttgcggatc cgcgagcagg
      661 agcgtgagct cagactccag cggatggaag aagaactccg catggcccgg accatccaaa
      721 agcagtttct tcccagaatt gttccggcag gacccggctt ccgcttttcg gccatctatc
      781 agccggccga gatggttggc ggcgatctgt atgattttta tatggaagaa acagcgtgta
      841 ttggattcat ctgcgatgtc tcgggtcacg gtgttccctc ggccctgatt tccggtctgg
      901 tcaaggcgac cttcgaagat gctgcctcga tggacggtga tccggggagt attctcacgc
      961 gaatgaatgg cttgctgcat gacaagatgg ggacgtattt tttaacggca gcctgttttc
     1021 gctatgacca gagcacacgg gatgtaacgc tggcatccgc ggggcacaat ccgcttctcc
     1081 tggtcaggga tggcagggtt gaggaattgg tggcacatgg tcgggtggtt gggccatttc
     1141 cggagtatgt ctatgcgacc gagacctgga gacttgagcc nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn tggttgggcc atttccggag tatgtctatg cgaccgagac
     1321 ctggagactt gagcccggtg atcgcatcct ggcctatacc gacggaatca acgaatgtgt
     1381 ccggttttca cctgtggaag aaatgtacgg tgacgagcgg ctgatggatt ttttggcgga
     1441 gaagacctca ttggatggcg agcaattttt gcaggtcttg ttggacacgc tcggcacttt
     1501 ctccgggaat gccccttttg aggatgacgt gaccgccatt gttctcgaca tcgaataaac
     1561 cgacgatact tcgggtttca cggtgttttg tattctgcat atcaacccca ttatggatac
     1621 tggatacatg aagagctaca ggaaagaact ctggtttgaa gcgaaacaga ggcgggaatt
     1681 gttcaatatc acgggacagg ttgaggactg cctcggggag agcgggatcc gggagggtct
     1741 ggctctcgtc aatgcgatgc acatcaccgc cagcgtgttc atcaacgacg acgagactgg
     1801 cttgcatcac gattttgagg tctggctcga aggccttgcc ccagaaaaac cctataatcg
     1861 gtaccggcac aacgggtacg aggacaacgg ggatgcgcac ctcaagcgga caatcatggg
     1921 gcgcgaagtc gtcgttgctg tcaccaacgg gaagctcgat cttggtccct gggaacagat
     1981 tttttacgga gagtttgacg ggatgcgccg caagcgcgtt cttgtcaaga tcatcggcga
     2041 ataaaccgtg ccatgcccgg ttcgggcatg acggatgatt cgatacacag caaccccgat
     2101 ggggtaaagg agtgaaacaa tgaaaaagat gttgagaatg accggggtgg tcgtgtccct
     2161 cgtggccatg attctcccac tgacggccta tcagtcttcg caggctgaac tggcgagacg
     2221 caagtcggct gttgaccgct atcgtgcggc aggcgtcttc agcgaaaagg tgtaccaggc
     2281 cgtcatgagt actccccgcg agtacttcat gccggagggg caaaaccaca tgacctacga
     2341 agaagttcct gtggcaatcg gctggggaca gaccatcacc aaccagtggt tcgtcgccta
     2401 tatgacccat ctcttggacg ttcaggcaag tgacaaggtg ctcgagatcg gatccggttc
     2461 cggctaccag gcctccatcc tctggcagat tacgaaaaag gtctacacca tcgagatcgt
     2521 cagccagttg gctgccattg ccaagcagcg ctggcagaat ctcgggtaca gctcgatcgt
     2581 tggcaaggcc gctgacggct attatggctg ggccgagtac ggtccgtttg acaagatcgt
     2641 cgtgacctgc gccgccgatc acgttccggt ctacctgatg cagcagctca agccgggcgg
     2701 ggtaatggtc attcccgtcg gcaatccctt tgaccgccag acactgctca agatcaccaa
     2761 aaacatggat ggaacggtcc gtaccgagcg cctgcagtcc gtaaaatttg ttccgttcac
     2821 gggcaagatg cttgagaaat acgggaagtg atgccttgat ggaaaaacgt ggtcttgtca
     2881 gtgttttcct ggtatcgatg gcggtactgg gtgccgagct gggtgtcatt cgctacatga
     2941 gtgtggcgag ctggtccaac tttgccggac tgatcatcag cgtggccctg ctcgggtttg
     3001 gtttgtcggg caccatcctg actgtcatgc gcgagcggat cgaacgccgg ctccaggact
     3061 ggcttttttg gtcggcagca gcctttgttg tgacgtccgg ctatgtcctg cagtttgcga
     3121 gagcgattcc ctttatcccg cagagtcttc atcaggacgg aacgcaggtg ctctatctgg
     3181 ctctctatta cttcctgttc ttcctgccgt ttttctgcgg tgcgctcttc atcaagcttt
     3241 cgttcatccg catgaaaacc ggcatcaaca ggatgtatct ctacaacctg atgggttccg
     3301 gagtcggggc gctggtt
//
LOCUS       DAOSHE010000226         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27044_length_3305_cov_3.116000, whole genome shotgun sequence.
ACCESSION   DAOSHE010000226 DAOSHE010000000
VERSION     DAOSHE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27044_length_3305_cov_3.116000"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1349)
                     /locus_tag="PLD82_06875"
     CDS             complement(144..1349)
                     /locus_tag="PLD82_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOG65142.1"
                     /translation="MDDIAKKIMELAEKYRDYTAQNLSKLVKIPSESMQEKEVQAELK
                     RQMEEAGFDEVRIDGLGNVIGRIGNGKKILAIDGHMDTVGVGNRDNWTFDPFSGEIKD
                     GFVHGRGTVDQEGGPAAAVTAGRILKELGFDRDLTFYVVGSVMEEDCDGLCWNWIIEE
                     EKIKPDFVISTEPTNLNIYRGHRGRMEIAVDFRGISCHGSAPERGKNAVYMASKVALE
                     IEKLNERLKDDEFLGKGTVTISVFESGSPSMCAVADFVHIHLDRRLTWGETKESAVKE
                     VEDVVTACGLKLGEEVKVYVMNYEETAYTGKRYGMEKYFPTWKIEPTEHVVTTGVDVF
                     KTLFNKEPKVDKWTFSTNGVTIRGKHGVPLIGFGPGNEVNAHAPNEKCPVDDLVKASA
                     FYAYYAYKI"
     gene            complement(1456..>3305)
                     /locus_tag="PLD82_06880"
     CDS             complement(1456..>3305)
                     /locus_tag="PLD82_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG65143.1"
                     /translation="FALGDTDKAWADCDLVIEGTAENGGQEHFYLETQIAVAVPQENG
                     EMKLISSTQSPTVVQKAAARVLGVPQHAVEVELKRLGGAFGGKEDQATAWAVMAALAA
                     RVTGKPAKICLDRREDITFTGKRHPYSTDFKIGLKKDGTIVAFQAMYYQNSGAVADLS
                     TSILERTLCHATNSYNIPHVRVTAAACYTNLPPFTAFRGFGGPQGMYVIEAAIHLAAE
                     KLGLPAHVIQRKNLLKEGDLFPYGQYTERCQAQRTWDALAARQDLPALQKRIDEYNAA
                     HTDSKKGFALMPICFGISFTTLFLNQAGALVHIYADGSVTVSTGVVEMGQGVHMKIMQ
                     VVAKTLSIDVRRVHTQTTNTFKVANTSPTAASSGADMNGKAAEIACSDILGRLKVAAA
                     AILGHNNPGDIQIRDEVVWCEGRETQLKWKELVQKAYLMKTNLSAYGHYAVPNLTFDK
                     SKEKGRPFAYHVYGTALIEATVDCIRGTYRFDAAHVVHDIGRSINELIDEGQVYGAMM
                     QGIGWVTLEELMYSKEGKPLVDTLSKYKVPDMRFTPETLSLQFLENADNPYAVMNSKA
                     VGEPPLMYGIGAYFAVLNALAAARPGRELFFSAPLTPKKVLSFLYDGAK"
BASE COUNT          666 a          978 c          899 g          762 t
ORIGIN      
        1 tagcccttga tctccttgat caatgcactg aaatctgcca tgtggtacct tcctgatttt
       61 tttcgtgatt cacaacaagc attgtatatc cacaaacgat cccggcaggg ataccgggat
      121 cgttcgcttc actcttttgt gtttcagatc ttgtacgcgt aatacgcgta gaatgctgat
      181 gccttgacca gatcgtccac cgggcacttc tcgttgggag catgggcatt gacttcgtta
      241 ccaggtccga acccgatcag cggaacgcca tgcttgccac gaatggtcac gccgtttgtg
      301 gagaatgtcc acttgtcgac cttgggttcc ttgttgaaga gggttttgaa gacgtccaca
      361 ccggtggtga caacatgctc ggtcggctcg atcttccagg tggggaagta tttttccatc
      421 ccgtagcgct tcccggtata ggcggtctct tcgtaattca tcacgtagac cttgacctct
      481 tcgcccagct tgagtccgca ggccgtcacg acgtcttcca cttccttgac ggccgattcc
      541 ttggtctcac cccaggtcaa gcggcggtca agatggatat gcacaaagtc ggccacagcg
      601 cacatggagg ggctgcccga ttcgaagacc gagatggtca cggtaccctt gccgaggaac
      661 tcatcatcct tgaggcgctc gttgagcttc tcgatctcga gagccacttt ggaggccatg
      721 tagacagcgt ttttcccgcg ctcgggagcg gaaccgtggc aggagatccc gcggaagtcg
      781 accgcgatct ccatgcggcc gcggtgtccg cggtagatgt tgaggttggt cggctcggtg
      841 ctgatcacga agtccggctt gatcttctct tcttcgatga tccagttcca gcagagacca
      901 tcgcaatcct cttccatgac ggaaccgaca acgtagaagg tcaggtcacg gtcaaagccg
      961 agttccttga gaatgcggcc agcggtcaca gcggcggccg ggccaccctc ctggtcaacc
     1021 gttccacgac catgcacgaa accgtccttg atctcccccg aaaagggatc aaaggtccag
     1081 ttgtcgcggt tgccgacacc gactgtatcc atgtgcccgt cgatcgccag aatttttttt
     1141 ccgtttccga tccggccgat cacgttcccg agtccatcga tgcggacttc gtcaaagccg
     1201 gcctcttcca tctggcgctt gagctcggcc tggacttcct tttcctgcat gctctcggaa
     1261 ggaatcttga caagcttgga aaggttttgc gcggtgtaat cgcgatactt ctcggcaagc
     1321 tccataatct tctttgcaat atcgtccata gttcgtcgct tctcctctga taagttctag
     1381 ccgctattgt aacacgagtc gacaagaaaa gcaaacgtcc tccggcggca cggaaaacat
     1441 tcccgttgaa aggtactact tggctccatc atacaggaag gaaagcactt ttttgggggt
     1501 aagcggggcg ctgaaaaaca gctccctgcc gggtcgggca gccgcaagcg cattcagcac
     1561 ggcaaaatag gcgccaatcc cgtacatcag cggtggttcc cccacggcct tggaattcat
     1621 caccgcgtac ggattgtccg cattctcgag gaactgcagg ctgagcgtct ccggggtaaa
     1681 ccgcatatcg ggcactttgt acttgctcaa ggtatccacc agcggctttc cttccttcga
     1741 gtacatcagc tcctcaaggg tgacccagcc gattccctgc atcatggccc catagacctg
     1801 gccttcgtcg atcagctcgt tgatactgcg accgatatcg tggaccacat gggccgcatc
     1861 aaaccggtag gttccgcgga tgcagtccac ggttgcctcg atcagggccg ttccatagac
     1921 gtggtaagca aaaggacggc ccttttcctt cgatttgtca aaggtcagat tcggaacggc
     1981 ataatgcccg taggccgaca gattggtctt cataaggtag gctttttgaa ccagctcttt
     2041 ccacttgagc tgtgtctccc ggccttcgca ccagacgacc tcatcccgga tctggatatc
     2101 cccgggattg ttgtgcccga ggatggccgc agctgcaacc ttgagccggc caagaatgtc
     2161 ggagcaggcg atttcggcgg ccttgccgtt catgtcggcc ccggagctgg ccgcggttgg
     2221 cgaggtattg gccaccttga aggtattggt cgtctgggta tgcaccctgc gcacatcgat
     2281 ggaaagcgtt ttggcgacaa cctgcatgat cttcatgtgc acgccctgcc ccatctcgac
     2341 caccccggtg gataccgtca cactcccgtc ggcataaatg tggaccaagg cgccggcctg
     2401 gttcaaaaag agcgtggtaa acgaaatccc gaagcaaatc ggcatcaggg caaagccctt
     2461 tttgctgtcc gtatgggcgg cattgtactc gtcgatccgc ttttgcaggg caggcagatc
     2521 ctgccgagcc gccaatgcat cccaggtccg ttgagcctga caacgctccg tatactgccc
     2581 gtagggaaag agatcaccct ccttgagcag gtttttccgc tgaatgacat gagccggcag
     2641 accaagcttt tcggccgcca ggtggatggc cgcctcgatg acatacatgc cctgtggtcc
     2701 gccaaatccc ctgaacgctg taaacggcgg aagattggta taacaggcgg cagccgtcac
     2761 cctgacatgg ggaatgttgt agctattggt cgcatggcag agtgtgcgct caagaatcga
     2821 ggtcgaaagg tccgccacgg cccccgaatt ttgatagtac atggcctgaa aggccacaat
     2881 cgtgccgtcc tttttgagcc cgatcttgaa gtccgttgaa tagggatggc gcttcccggt
     2941 aaacgtgatg tcctcgcgtc gatccagaca gatcttggcc ggcttgcccg taacccgggc
     3001 agcaagagcc gccatgaccg cccaggccgt cgcctgatcc tccttgcccc cgaaggcgcc
     3061 ccccaggcgt ttgagttcaa cttcaacggc atgctgggga acaccgagca cccgggcggc
     3121 ggccttctgg accactgtgg gtgactgggt cgaggagatg agcttcatct cgccattttc
     3181 ctggggtacc gccaccgcga tctgggtttc gaggtaaaaa tgctcctgtc ccccgttttc
     3241 ggccgtccct tcaataacaa ggtcacagtc ggcccaggcc ttgtccgtgt cgcccagtgc
     3301 aaaag
//
LOCUS       DAOSHE010000227         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27148_length_3296_cov_4.630052, whole genome shotgun sequence.
ACCESSION   DAOSHE010000227 DAOSHE010000000
VERSION     DAOSHE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27148_length_3296_cov_4.630052"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2176)
                     /gene="clpA"
                     /locus_tag="PLD82_06885"
     CDS             complement(<1..2176)
                     /gene="clpA"
                     /locus_tag="PLD82_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOG65144.1"
                     /translation="MLSKDLQLSIFAAFREAKRRRHEFLTVEHITSALLFNELAQYIL
                     ESLGIDVEGLRTTMDTFLERTTEKLPEGIKRDPIETPAFQRVIQRAMAHMQSAGKESV
                     DAGDMLVSIMHEEDSHSFYYLTQAGLTRLELMQFVSHGPSPDHQPPQTDDDSDREQGN
                     LPAKSALEQFCTNLNAKAARGDVDPLIGRNPEMRRVQQILCRRQKNNPILVGDPGTGK
                     TALAEGLALAISEGRVPAILREFTVFSLDLGSLVAGTKFRGQFEERLKGVMNELVRHE
                     NAILYIDEIHTIIGAGSTSGGNLDASNILKPALASGKMRCMGSTTHEDYRQIFDKDRA
                     LSRRFQTIHIEEPSQSEALQILRGLKSRYEQHHHIRYTDAALKTAVELSAKYITDRRL
                     PDKAVDVIDEAAAALHLLPPSAQRRRVVPTDIEAVVSLIARVPVQTDDTNMTAAMASL
                     PAALGGIVFGQDEAVLTLTRALRRSRAGLGHPDHPLGSFLFTGPTGVGKTEVCRQLAK
                     QLGIELLRFDMSEYMEKHAVARLIGSPPGYVGFEQGGLLTEAVRRTPHAVLLLDEIEK
                     AHEDIFNILLQIMDHAALTDNTGRKSDFHNIILVMTSNAGAREMSQSAIGFGDPHGEA
                     LNKGRSAIEKLFSPEFRNRLDETIVFKPLTAEVMLLVVDKFVNLMRPGLRARGITIDL
                     DDKAREFLATRGYDPVYGARPLDRLIQTSIKDPLSEEILFGRL"
     gene            complement(2179..2484)
                     /locus_tag="PLD82_06890"
     CDS             complement(2179..2484)
                     /locus_tag="PLD82_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOG65145.1"
                     /translation="MTEGKQESEQAVSTVNREPPRFRVLLHNDHFTTMDFVVEILETV
                     FHKRPHEAMAIMRHVHERGIGVCGIYTREVAEARVALVTRLAHENEFPLLCTMEREE"
     gene            2732..>3296
                     /locus_tag="PLD82_06895"
     CDS             2732..>3296
                     /locus_tag="PLD82_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG65146.1"
                     /translation="MSLRAISGGLLLGFLCLSCASGDAGRDGSVLPEAQVKREKKVGL
                     ALGVSGLGDQSFNDMQYSGLVQSYERMPIQAAFRVPASNEEKDLVTLFETLITKDQCN
                     LVIVAEAYAMGEVVKKFAEKHPDVMFVLMESAAVDRPNIAATEFAQNEGSFLAGMLAA
                     RVSKAKRFGFIGGVDLLPVKDFLTGYQA"
BASE COUNT          620 a          871 c         1029 g          776 t
ORIGIN      
        1 atagccggcc gaagaggatc tcttcggaga gggggtcctt gatgctggtc tggatcagcc
       61 ggtcgagggg acgggcgcca tagaccgggt cgtacccgcg ggttgccaga aactcgcgtg
      121 ctttatcatc cagatcgatg gtgatccccc tggcacgcag gccgggtcgc atcagattga
      181 cgaatttgtc gacgacgaga agcatgacct ccgctgtcag cggcttgaag acgattgtct
      241 cgtcgagccg gttgcggaac tcgggggaga agagcttttc gatggcgctc cttcccttgt
      301 tcagggcttc gccatgtgga tctccgaatc cgattgcgga ctggctcatc tcccgggcgc
      361 cggcattgct tgtcatgaca agtatgatat tgtggaaatc ggatttacgt ccggtgttgt
      421 cggtcagggc ggcatgatcc atgatctgga ggaggatgtt gaagatgtcc tcgtgggctt
      481 tttcgatctc gtccaggagc agcacggcat gcggggtccg ccggacggcc tcggtcagga
      541 gtccgccctg ttcaaagccg acataccccg gcggagatcc gatgagtctt gccacggcat
      601 gtttttccat gtactcgctc atgtcgaagc ggaggagttc aatcccgagc tgtttggcaa
      661 gctggcgaca gacctcggtt ttgccgaccc cggtcggacc ggtgaagagg aagctcccca
      721 ggggatgatc gggatgtccc agcccggccc gtgaacggcg gagggcacgg gtgagagtca
      781 aaacagcttc gtcctgcccg aagacaatgc cacccagggc tgccggaaga ctggccatgg
      841 ccgccgtcat gttggtatcg tccgtctgga caggtacccg ggctatcagg ctgacgaccg
      901 cttcgatgtc ggtgggcaca accctgcggc gttgggctga tggtggcagg aggtggaggg
      961 ccgcggcggc ctcgtcgatc acatcgacgg ccttgtccgg gagtctgcga tcggtgatgt
     1021 atttggccga gagttccacg gcggttttga gggctgcgtc cgtatagcgg atgtgatgat
     1081 gttgttcata gcggctcttc agcccgcgga ggatctgcaa ggcttcgctc tggctgggtt
     1141 cctcgatgtg gatggtttga aagcggcggg agagtgcccg gtctttgtcg aagatctgcc
     1201 ggtaatcctc atgtgtggtc gagcccatgc accgcatttt tccgctggcc agagccggtt
     1261 tgaggatgtt ggatgcatcc agattgccgc cgctggttga acctgcaccg atgatggtgt
     1321 ggatttcatc aatgtagagg atggcatttt catgcctgac cagttcgttc atgacaccct
     1381 tgagacgctc ctcaaactgg ccccggaatt ttgttccggc taccagcgat cccaggtcga
     1441 gtgaaaaaac cgtgaattcg cgaaggatgg ccgggacccg gccttcactg atcgccagcg
     1501 ccagcccttc ggccagagcc gttttgccgg ttccgggatc tccgaccagg atcgggttgt
     1561 ttttttggcg tcgacagagg atctgctgga cgcggcgcat ctccgggttt ctcccgataa
     1621 gcgggtccac atccccccgg gcagccttgg cgttgagatt ggtgcagaat tgctcaaggg
     1681 ccgacttggc cgggagattt ccctgttccc tgtcggaatc atcgtctgtc tgcgggggct
     1741 gatggtccgg agacggtccg tgcgagacaa attgcatcag ctcgagtctg gtcagtcctg
     1801 cctgggtcag atagtagaag gaatgggagt cctcttcgtg catgatggag acgagcatgt
     1861 caccggcatc cacactctcc ttcccggctg attgcatatg ggccatggca cgctggatga
     1921 cccgttggaa ggccggggtc tcgatcgggt cacgcttgat tccttcgggg agcttttccg
     1981 ttgtccgttc gaggaaggta tccatggttg tccgcaaccc ctcgacgtca ataccaagag
     2041 attcgaggat gtactgcgcc agttcgttga agagcagggc ggaggtgatg tgctcgactg
     2101 tcagaaactc gtgacgccgg cgcttggcct cacgaaaggc ggcgaagatg gagagttgca
     2161 gatccttgct gagcatgctt attcctcccg ttccatcgtg cagagcaacg ggaactcgtt
     2221 ttcgtgtgcc aaccgggtga ccagtgccac cctggcttcg gcgacttccc gggtatagat
     2281 gccgcagacg ccgatgccac gttcgtggac atggcgcatg atggccattg cttcatgcgg
     2341 ccgcttgtga aagacggtct caagaatctc gacgacaaag tccatggtgg taaagtggtc
     2401 attgtggaga aggacgcgaa agcggggtgg ttcgcgattg acggtcgaaa ctgcctgttc
     2461 gctctcctgc ttgccctcgg tcatcctgtt ttcctccact cacatccggt cttgatcggc
     2521 tggaagccgt acacagcagt acaacggcgt ttccatacag ggatggcacc ggagaaaact
     2581 ccgttcggaa ataccgtaaa gcatccattt caaaatctag ccccttttgg gcatcttgtc
     2641 aaattgtcat ggttgtacat ggaattgtat gccgttccgc catgaaaaga ctatggcaat
     2701 ccctgacata tgccgaaaaa gaaatatgaa catgtctttg cgggccattt cgggcggtct
     2761 tctcctggga ttcctgtgtc tctcctgtgc ttcaggtgat gcggggcggg acgggtccgt
     2821 gcttcccgaa gctcaggtca aacgtgaaaa aaaagttggt ctggcccttg gtgtctccgg
     2881 tcttggcgac cagtctttca acgatatgca gtactcgggt cttgtccagt cgtatgaacg
     2941 gatgccgatc caggctgcct tccgtgttcc tgccagcaat gaggaaaaag acctcgtcac
     3001 tctgtttgag accttgatca caaaggatca gtgcaatctg gtcattgttg ccgaagccta
     3061 tgccatggga gaggtcgtca aaaaatttgc ggagaagcac ccggatgtga tgtttgtcct
     3121 gatggagtct gcggcggttg accgcccgaa tattgcggcc accgaattcg cccagaacga
     3181 ggggtccttt ttggccggga tgcttgctgc ccgggtctcg aaagcgaagc ggttcggctt
     3241 cattggcggg gttgatctct tgccggtcaa ggattttttg acggggtacc aggccg
//
LOCUS       DAOSHE010000228         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27237_length_3290_cov_3.370634, whole genome shotgun sequence.
ACCESSION   DAOSHE010000228 DAOSHE010000000
VERSION     DAOSHE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27237_length_3290_cov_3.370634"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLD82_06900"
     CDS             <1..1116
                     /locus_tag="PLD82_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65147.1"
                     /translation="RAAVSGTDEIMLLASAFNMMAGRAEDLVGRLRSEKVLLEQILAT
                     MTDSIVVLDPAGGLILWNEAFCRMFPGGYIRAGMRYYEVMDASRVLASIEGVLATGEA
                     LSFSAELPRRMETRVEVQCLPLGGNAGLLVLLRDSTERYQYEKMKAEFAANASHELKT
                     PLAIMDGYVQTLLASPQPDEVTRIRFTQRIASSLDRLRELVSDIILLNRLEEGGHEPK
                     MEQVDLVKTLERILSSLQPRLEASGVRVVPVRIPVRAIVRGNPALLDSLFFNLMENAL
                     RYSQTGGRVEIVVEILTGSVETRIRDEGCGFFPGEEERIFERFYRGEGSRARETGGSG
                     LGLAIAKYIVRYHAGTITASSPGPGLGAEFVVVLPFG"
     gene            1243..>2011
                     /locus_tag="PLD82_06905"
     CDS             1243..>2011
                     /locus_tag="PLD82_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65148.1"
                     /translation="MIGWAKYRLEGGWKMRASIGQRLRYAVENFMSKGGKAVFLSLTL
                     LFLLALVLVLVIRLFAMHGGTDAGAALDDTWKVMTQLIDTGTVTAEQESVEKIIGMVA
                     TILGVVIFSMLIAFITTRLDLILYNLRRGKSRVLETNHTLILGWSDRVMDILRELIIA
                     NESESRAVVVILADADKELMDETIHAQLPDTKTTRVITRTGCPSSLRDLARVNASGAR
                     SAIVLATCSEYASDGDKEISDTLSIKVILALQAMWRGG"
     assembly_gap    2012..2021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2022..3219
                     /locus_tag="PLD82_06910"
     CDS             <2022..3219
                     /locus_tag="PLD82_06910"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65149.1"
                     /translation="FPIIAEIYDAAKRDLVNADESGKVLSIDSWEIMGRLFAQTSLTS
                     GLEVVYNEMLSFDGCEVYFYKADWNGVRFGDLPGHFKDGVPLAIHDPASGAITTKPPR
                     DHVMTDGEEIIILAQDDSSIKFSKKKLYSPKPCPGLGNHQKRNAKRVLILGWHPIGNV
                     VVREYANYLGAGSTVHIVHRSPDQGLEDFVRGGVGEALALSLRTGDPRQLEVLQELKP
                     FEYDTILILSQDAGQFDAEKVDGDTLMILLILRRLAAYSEDGFKTTTILTQVFNSENQ
                     ELLARTDADDFIISNRLITMMLAQLSEQPGIKRFYDDLFSEEGSEIYVKPATLYFDSF
                     PVTRTFIDLMACADARDEICLGWRIGALRRESTQNFGVTLDPPKTASVTLNADDFLVV
                     LADDAG"
BASE COUNT          693 a          844 c         1018 g          725 t
ORIGIN      
        1 cgggcagcgg tctctgggac ggacgagatc atgcttttgg catcggcctt caatatgatg
       61 gccggccggg cggaggacct ggttggaagg ttgcgttcgg agaaggtttt actggaacag
      121 atccttgcca ccatgaccga ctcgatcgtc gtgctggatc ctgccggcgg gctgattctc
      181 tggaacgagg ctttttgccg gatgtttccc ggaggataca tccgggccgg gatgcgatac
      241 tatgaagtga tggatgcgtc ccgggtgctt gcatccattg aaggcgttct tgcaacaggg
      301 gaggcgctct ccttcagcgc ggaactcccc cgcaggatgg agactcgggt cgaggtccaa
      361 tgtctgcccc tcggcgggaa tgccggcctg ttggtgcttc tccgggacag tacggaacga
      421 taccaatatg aaaaaatgaa agccgaattt gcggccaatg cgtcgcacga actcaagacg
      481 ccgctggcca tcatggatgg ctatgtccag acccttcttg cgagtcctca gccggatgaa
      541 gtgacccgga tccggtttac acaacggatc gcctccagcc tcgaccgttt gcgggagctt
      601 gtgtccgaca tcatcctgct gaacaggctg gaggagggcg ggcatgaacc aaagatggag
      661 caggttgatc tggtcaaaac tctggagagg atcctctcct ccctccagcc acggctggaa
      721 gcctctgggg tccgggttgt accggtgcgc ataccggtcc gggccattgt ccggggcaat
      781 ccggccctcc tggattccct ctttttcaac ttgatggaaa atgccctccg ctacagccag
      841 accggaggcc gggtggagat tgtggtcgag atcctgaccg gaagtgtcga gaccaggatc
      901 cgtgatgagg gatgcggatt ctttcccggt gaggaagaaa ggatttttga gcgcttctac
      961 cggggggaag ggagccgggc cagggagaca ggggggagcg gactggggct ggcaattgcc
     1021 aagtacattg tgcgctacca tgccgggaca atcaccgcca gcagtcccgg acccgggctg
     1081 ggagcggaat ttgtggtcgt cctgcctttc ggctgagtgc tgatgaaaac gagctttgta
     1141 tacgatgcat gagtctgtgt atacggtgtg ggttcggact catggagagt cgttcatctg
     1201 ccggcaagac atgtcgaagc ggttgccatc gggcgaagaa atatgatagg atgggccaag
     1261 tatcgtctgg aggggggctg gaaaatgcgt gcatccatcg gacaacgtct gcgctacgct
     1321 gtcgagaatt tcatgtcaaa gggcggcaag gctgtttttc tcagtctgac cctgttgttt
     1381 ttgttggcct tggttcttgt tctcgtgatc cggctctttg ccatgcatgg cggaaccgat
     1441 gccggggcgg ccttggatga tacatggaaa gtcatgacac aattgatcga taccgggact
     1501 gtgacggccg agcaggaatc ggtcgaaaag atcatcggga tggtggcgac gatcctgggt
     1561 gtggtgattt tttccatgct gatcgccttc atcacaacgc gtctggatct gatcctctat
     1621 aatttgcggc gaggaaagag ccgggtcctt gaaacaaacc atactctgat cctcggatgg
     1681 agtgaccggg tgatggatat cctcagggaa ctgatcatcg ccaacgagag cgagtcgagg
     1741 gccgtcgtcg tgattttggc cgatgccgac aaggaattga tggacgagac catccacgcc
     1801 cagttgccgg acacaaagac gaccagggtg atcacccgga ccggctgtcc ttcttccctg
     1861 cgggacctgg cacgggtcaa tgcttccggt gccagatcgg cgatcgtgtt ggcgacttgt
     1921 tcggagtacg ccagtgacgg ggacaaggag atctcggata ccttgtccat caaggtcatc
     1981 ctcgccctgc aggcgatgtg gcgaggaggg gnnnnnnnnn ngttcccgat cattgccgag
     2041 atctatgatg ccgccaaacg ggatctggtc aatgccgacg agagcgggaa ggtcctgtcg
     2101 atcgacagtt gggaaatcat gggacgactc tttgcacaga cctcgctgac gagcgggctc
     2161 gaggttgtgt acaacgagat gctttcgttc gacggatgcg aggtctattt ttacaaggcc
     2221 gactggaacg gagtccgttt cggggatctg ccgggtcact tcaaggatgg cgtcccgctg
     2281 gcgattcatg acccggccag tggcgccatc accaccaagc ctccgcggga ccacgtcatg
     2341 acggatggtg aggagatcat cattctggct caggatgatt cgtcgatcaa attctcaaaa
     2401 aagaagctct attcccccaa gccctgtccc ggactgggga atcaccagaa acggaatgca
     2461 aaacgtgtcc tgattcttgg ttggcatccg atcgggaatg tggtcgtgcg tgagtatgca
     2521 aattatctcg gggcgggttc gactgtccac atcgtccatc gttcaccgga tcagggattg
     2581 gaagattttg tccgtggagg cgtcggcgaa gcacttgccc tctcgctccg gaccggagat
     2641 ccccggcaac tcgaagtgct gcaggaattg aaaccctttg aatatgacac gattctgatt
     2701 ctttcacaag acgccgggca gtttgatgcc gaaaaagtcg acggggatac cttgatgatc
     2761 ctcctgattc tgcggaggct ggcagcgtat tcggaggacg gcttcaagac gacaaccatt
     2821 ctcacccagg tcttcaactc cgaaaaccag gaactcttgg cacggacgga tgcggacgat
     2881 ttcatcatca gcaaccgtct gatcacgatg atgctggccc agttgagtga acaacccggg
     2941 atcaagcgct tctatgatga ccttttcagc gaagagggga gcgagatcta tgtcaaaccg
     3001 gcgacgctct atttcgacag cttcccggtc accaggacat ttattgatct gatggcctgt
     3061 gccgatgcgc gggacgagat ctgtctcggg tggcggattg gcgcattgcg acgggagagt
     3121 acacagaact ttggtgtgac ccttgacccg ccaaaaaccg caagcgtcac gctgaatgcc
     3181 gatgattttc tcgtcgtcct cgccgacgac gcgggctaaa acaggatgtt ttgtcctgag
     3241 cgggtccatg gcgaagtacc cggaacgcag tgtagggtaa tggaccctta
//
LOCUS       DAOSHE010000229         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27307_length_3284_cov_3.441623, whole genome shotgun sequence.
ACCESSION   DAOSHE010000229 DAOSHE010000000
VERSION     DAOSHE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27307_length_3284_cov_3.441623"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2236)
                     /gene="alaS"
                     /locus_tag="PLD82_06915"
     CDS             complement(<1..2236)
                     /gene="alaS"
                     /locus_tag="PLD82_06915"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG65150.1"
                     /translation="MKRSQDIRNDFLEFFRSKGHTVVPSASLLPVGDKTLLFTNAGMN
                     QFKDIFLGQGRREYTRAADTQKCMRVSGKHNDLDDVGRDTYHHTFFEMLGNWSFGDYY
                     KKEAIGWAWELLTSVWKLHKDKLYATVYREDGEAYELWKSETDIAHDHIMYFGEKDNF
                     WEMGETGPCGPCSEIHIDQGEGTCNMQHVAGHVCGVNAGCSRYIELWNLVFIQYNRQT
                     DGSLEPLPSKHVDTGMGFERVTAVLQGEKSNYDSDIFRPLLETIGSMTGRSYGKSTSI
                     EDIAMRVIADHIRSLSFSIADGIMPSNDGRGYVIRRILRRALRFGQKLGLEKPFLAQL
                     VPILEQTMGGIFPELGRNSSRIVEVLEHEETRYFTTLDAGMRELESMLQAAKKDGSLL
                     LAGDKIFHLYDSLGFPADIAEEVAKDEGVQADMEGFAALMEEQKNRGRQSWKGAHAEA
                     LTSIPRDMAPTDYRGDREFESRTSVLCLATDEAAVTRLDAGTEGWLIADATPFYAEGG
                     GQIADTGTLTGGSGTALVLDVTRRDAIYLHRVRVESGCLSVGDGITLVVEEERKRAIA
                     RNHTATHLLQQALVEVLGDHVAQAGSHVSAERLRFDFSHFRAIEESDLVRVEEIVNRI
                     VRQNIDVSKEIMDRETAVSRGAKAFFGEKYGETVRVVTVGGFSMELCGGTHVDRTGDI
                     GAIRILSETSVSSGVRRIEAVTGQAALRKGQEDARTIALLSSMLNVPTEKLVERIESL
                     VEQGR"
     gene            complement(2352..3005)
                     /locus_tag="PLD82_06920"
     CDS             complement(2352..3005)
                     /locus_tag="PLD82_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG65151.1"
                     /translation="MSGSSLEIMICDLHPLDGGEYDVTDSDGFVWLFHTRVVEVFNLD
                     EGRLVASETLMHAAVASSLLRARMLVRTILGRTRKPVPRLETDLRSRGIGEVDADCAL
                     RLALLTGLVDDLGWARDLVEIRHERRGEGPAAIRFWLEQRGFDRDVIRQALEHLAELA
                     DPLETACALLAERFPAGSADPALRQKAWGFLARRGHSFEVIGKALRRHFSGSDPDDW"
     gene            complement(3005..>3284)
                     /locus_tag="PLD82_06925"
     CDS             complement(3005..>3284)
                     /locus_tag="PLD82_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65152.1"
                     /translation="QQYRIGITISNYGIISEDTRMKLAKKMNADVEGISLDSFYTEKG
                     EDKLGAGLGLLYNSYLEEICHKEGLLYRCNIFPEPEKEKTTVRLDITL"
BASE COUNT          666 a          999 c          921 g          698 t
ORIGIN      
        1 ttcttccctg ttcgacgaga gattcaatcc gctcaacgag tttttccgtc gggacattga
       61 gcatggacga gaggagtgcg atggttcgtg catcctcctg acccttgcgc agggcagcct
      121 ggccggtgac ggcctcgatc cgtcggactc ctgaagaaac cgatgtctcc gagaggatcc
      181 ggatcgctcc gatgtctccc gtccggtcga catgggttcc accacacaat tccatggaaa
      241 acccgcccac ggtgaccaca cggactgtct cgccatactt ctccccgaaa aaagccttgg
      301 cgccccgtga aaccgcggtc tcccggtcca tgatctcttt tgaaacatcg atattctgcc
      361 ggacaatccg gttgacgatt tcctcgacgc gtaccagatc gctttcctca atcgccctga
      421 aatgggagaa gtcgaagcgc aggcgctcgg ccgaaacatg tgatccggcc tgggcgacat
      481 ggtcacccaa aacctcgacg agcgcctgtt gcagaagatg ggtggccgta tggttccggg
      541 caatggcccg cttgcgttct tcctcgacga ccagcgtgat cccgtcaccg accgagaggc
      601 acccgctttc aacccggacc ctgtggagat agatcgcgtc acgtcgtgtg acatccagca
      661 ccagagccgt tccggacccg ccggtcagag tcccggtatc ggctatctgg ccgccaccct
      721 cggcataaaa gggcgtggca tcggcgatca gccagccctc ggtaccggca tccagccggg
      781 tcactgcggc ctcatcggtc gcgagacaga ggacactggt ccggctctca aactcccggt
      841 caccccggta gtcggtgggt gccatatccc tcgggatcga agtcaaggcc tcggcatggg
      901 cacccttcca gctctgccga ccacggtttt tctgttcttc catgagggcg gcaaagccct
      961 ccatgtctgc ttggacgccc tcgtccttcg cgacctcttc agcgatgtcg gccgggaacc
     1021 cgagggagtc atagaggtgg aaaatcttgt ctccggcaag aagaagcgac ccgtcctttt
     1081 tggcggcctg gagcatcgat tcgagttcac gcatgcccgc atcaagcgtc gtgaaatatc
     1141 tggtttcctc atgctcgaga acctcgacaa tccggctgga gttccggccg agttccggga
     1201 aaatcccgcc catcgtctgt tcgagaatcg gaacgagctg ggcgaggaag ggtttttcaa
     1261 gaccgagctt ctgcccgaaa cgcaaagccc ggcggaggat gcgcctgatg acatagccgc
     1321 ggccgtcatt tgacggcatg atcccgtcag cgatggagaa cgagagtgac cggatatggt
     1381 cggcgatgac acgcattgcg atatcctcga tcgatgtgct cttcccgtac gaccgaccgg
     1441 tcatggaacc gatcgtttcg agaagtggcc tgaagatatc cgaatcgtaa ttcgatttct
     1501 cgccctggag gaccgcagtc acgcgttcaa agcccatccc ggtatccacg tgttttgacg
     1561 ggagcggttc gagactcccg tctgtctggc ggttgtactg gatgaagacg aggttccaga
     1621 gttcgatata gcggctgcag ccggcattca ctccgcagac atggcctgca acatgctgca
     1681 tgttgcaggt gccctcaccc tggtcgatat ggatctccga gcacgggcca caaggcccgg
     1741 tctcgcccat ctcccaaaaa ttgtccttct cgccaaaata catgatgtgg tcatgggcta
     1801 tatcggtttc ggatttccag agttcatacg cctcgccatc ctcgcgatag actgttgcgt
     1861 agagtttgtc cttgtgcagt ttccagaccg atgtgaggag ttcccaagcc cagccgatgg
     1921 cttccttctt gtaataatcc ccgaaggacc agttgcccag catctcgaaa aatgtatggt
     1981 ggtaggtatc ccgtcccaca tcgtccaggt cattgtgctt gccggagacc cgcatgcatt
     2041 tttgggtatc ggcagcccgg gtgtattccc ggcggccctg cccgagaaaa atatccttga
     2101 actggttcat cccggcattg gtgaagagca gggtcttgtc cccgaccgga aggagcgagg
     2161 ccgagggaac aacagtgtgc cccttgctgc ggaagaattc gagaaaatcg ttgcgtatat
     2221 cctgggagcg tttcatgtcc tgtccatttt cctgttcatc ggcattgcac gagcgtctgg
     2281 ctcctgcgta cctgaaagta tcgtattttt ccattttccg gtcaattccc gcatgcgcaa
     2341 cgcgtgcggg atcaccagtc atccggatcg gatccggaga agtgccgccg gagagccttg
     2401 ccgatcactt cgaaagaatg cccccgccgg gccagaaagc cccaggcttt ctggcgtaag
     2461 gccggatcag cactcccggc cgggaaccgt tcagccagga gcgcacaggc cgtctcgagc
     2521 ggatcggcca gttctgcaag atgctcgagt gcctgacgga tcacatcccg gtcaaagccc
     2581 cgctgttcaa gccagaagcg gatggcggcc ggcccctccc cccgccgctc atgccggatc
     2641 tcgacgagat ccctggccca gcccaagtca tcaaccagac cggttaaaag cgccagacgg
     2701 agggcacagt cagcgtcgac ctctccgatc ccccgtgaac ggagatcggt ctccagccgg
     2761 ggaaccggtt ttcgggtcct gcccagaatg gtccggacga gcatcctcgc cctgaggagg
     2821 gaggatgcca cagccgcgtg catcagggtt tcggacgcaa ccagcctgcc ctcatcgagg
     2881 ttaaagacct cgaccacccg ggtatggaaa agccagacaa acccatcact gtcggtcaca
     2941 tcgtattcac caccatccag cggatgcagg tcgcaaatca tgatttcaag ggaactcccg
     3001 gacatcagag agtgatatcc agacggacgg tcgtcttttc cttttcgggt tcgggaaaga
     3061 tgttgcagcg gtacagcaag ccttctttgt gacagatctc ttcaaggtag gagttgtaga
     3121 gcaggccgag accggcgccc agtttgtcct cccccttttc ggtataaaag gagtccagcg
     3181 agatgccctc gacatcggcg ttcatctttt tggccagctt catcctggta tcttcggaga
     3241 tgatcccgta gttggagatg gtgatcccga tccggtactg ttgg
//
LOCUS       DAOSHE010000230         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27512_length_3267_cov_4.679950, whole genome shotgun sequence.
ACCESSION   DAOSHE010000230 DAOSHE010000000
VERSION     DAOSHE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27512_length_3267_cov_4.679950"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLD82_06930"
     CDS             complement(<1..618)
                     /locus_tag="PLD82_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65153.1"
                     /translation="MTINELARTLADRGHALWYIRTTALDSLHGIRAPRAVLELGTDA
                     ELVELARALDQIEYPGFPMESARFTTDSGQRISISCHLEDAPPPPYMELDARVPVVGD
                     KPQGLFFDEQSPGQALPAGTRPGSNCPPARAACEAARLIARYGYRPPPAIPVSGAAGL
                     PKTVLRELLTDILSARAVGLALDWLHKTGFLTDAFPELSGLHAVSH"
     gene            complement(635..2110)
                     /locus_tag="PLD82_06935"
     CDS             complement(635..2110)
                     /locus_tag="PLD82_06935"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG65154.1"
                     /translation="MPSIELVMVIASFLLLLSIVASKISARVGVPALLLFILIGMLTG
                     SDGSGGLWFDNYKLASNISVIALALILFSGGLDSSLNEIRMVFWPGFILSTVGVLATT
                     GTIAIGAHLITGFDLMHSILIGAIISSTDAPAVFGILRSRQIGIPQRLRSLLEFESGS
                     NDPMAICLTLGMVTILSIPSAGIDTMVGMLLMQLAIGAVIGWLAGKSFVFVIHRFRLE
                     YEGLYPILTSACAIGTYGLTAVAGGSGYLAVYLMGIVIGNGSFTHKRSILRFHNAISW
                     LMQIILFFTLGLLAFPAEIPSIALPAIGLSLFIMFVARPIVVALCLRPFGESWRNIAF
                     SGWVGLKGAVAIVLATLPLSTGITGAHEIFNLVFVVVILSLLFQGSTIPWVAKKLGLA
                     LPFKEKKNSPISFETSSEISSDMVELELHDCSTVIGMRLRDLSLPEDTLVALIGRAGQ
                     YIVPRGDTVLEKGDTIFVLAAKGHIESIRDAVSKRTKTSCT"
     gene            complement(2379..>3267)
                     /locus_tag="PLD82_06940"
     CDS             complement(2379..>3267)
                     /locus_tag="PLD82_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOG65155.1"
                     /translation="VLAATGKYEDGETRNDQMAPIPDTVFGLIEQAVKEKRTISEKNY
                     YIGFFRSKNGVENIIYLERTEDFDQWEKNLIDIFCSNVAIAFDNIYLNQEIEETQKEI
                     IFTLGEIAEGRSPETQHHVQRVSEYSVWLGRKLGLPEKDLETLRLASAMHDIGKLAIP
                     DAVLLKPDRLSVTERAEIQKHAPAGEEMLRFSKRSLIRVAAQIAAQHHENWDGSGYPH
                     GLAGESIDLFARITAVADVFDALSSDRVYRKALPPGEVEAYMREQSGRQFDPRIASLL
                     LDNIGEVRKLGENLAFIEG"
BASE COUNT          687 a          840 c         1061 g          679 t
ORIGIN      
        1 gtgagacacc gcatgcagtc ccgagagttc cgggaaggca tccgtcagaa accctgtttt
       61 gtggagccaa tccagtgcga gcccgactgc gcgggcggag aggatgtcgg tcaggagttc
      121 ccgaaggacg gtcttgggga gtccggctgc gccactcacg gggatggctg gtggtgggcg
      181 gtatccgtag cgggcgatca ggcgtgcagc ctcgcaggcg gcccgggccg gaggacagtt
      241 acttccgggg cgtgttccgg ccgggagtgc ttggcctgga gactgttcat cgaaaaaaag
      301 accctggggc ttgtcgccga caaccgggac acgggcgtcc aattccatgt agggcggagg
      361 cggggcatcc tccagatggc aggaaatgga aatcctttgg ccggagtccg tcgtgaatcg
      421 ggcgctctcc atcggaaaac ccgggtattc aatctggtcc agtgcccggg caagctcgac
      481 cagttcggca tcggtcccca gttcgaggac agcgcgagga gcacggattc cgtgcaggct
      541 gtccagggcc gtggtccgga tgtaccagag ggcatgaccg cggtccgcaa gtgtgcgagc
      601 caattcgttg atggtcatct cggctgttct ccggtcaggt acagcttgtc tttgtccgct
      661 tcgagacggc atcccggatg ctttcgatgt gccccttggc ggccaggaca aagattgtgt
      721 cgcctttctc gaggaccgtg tctccccggg ggacgatgta ctgtcctgca cggccgatca
      781 gcgcgaccag ggtatcctcc ggaagcgaga gatcccggag ccgcatgcca atgaccgtgg
      841 aacagtcatg cagttcgagc tcgaccatat cgctggaaat ctcgcttgag gtctcgaatg
      901 aaatcgggct gtttttcttc tctttgaatg ggagggccag gccaagtttt tttgccaccc
      961 aggggatggt ggagccctgg aagaggagag acaggattac gacaacgaag accaggttga
     1021 agatttcatg tgcaccggtg attccggttg agagcggtag ggtggcgagc acgatggcga
     1081 ctgcgccctt cagtccgacc caaccggaaa aagcaatatt tctccaagat tctccaaaag
     1141 gccggagaca gagtgcaacg actatcggtc gcgcgacaaa catgataaag agggacaagc
     1201 cgatcgccgg gagcgcgatg gagggaattt ccgccgggaa ggcgagcagg ccgagcgtga
     1261 agaagagaat gatttgcatc agccaggaga tggcgttgtg aaagcgcagg atggatcgtt
     1321 tgtgggtgaa ggatccgttt ccgatcacta ttcccatcag gtagacggca agatagccac
     1381 ttcctccggc cacggccgtc aggccatagg ttccgattgc gcaggctgat gtcaggatcg
     1441 gatagagccc ttcgtattcg agccggaaac ggtgaatgac gaagacaaag gacttcccgg
     1501 ctagccagcc aatgactgcg ccgatggcga gctgcatcag aagcattccg accatggtgt
     1561 ctatgccggc ggacggaatc gagaggatgg tgaccatgcc cagtgtcagg cagatggcca
     1621 tcgggtcgtt gctgccggac tcgaattcaa gcagagagcg cagtctttgg gggatgccga
     1681 tttgtcttga acggaggata ccgaagacgg ccggggcatc agtcgaggaa atgatggcac
     1741 cgatcaggat gctgtgcatc agatcgaatc cggtgatcag atgggcgccg atggcgattg
     1801 tgcccgtggt tgcgagcact ccgacggtcg agaggatgaa tccaggccag aagaccatcc
     1861 ggatttcgtt gagggaagag tctaggccgc cggagaagag gatcaaagcc agggcgatca
     1921 cgctgatgtt gcttgccagt ttgtaattat caaaccagag tccgcccgaa ccgtccgagc
     1981 cggtgagcat cccgatcaga atgaagagga gcagggccgg gacaccgaca cgggcggaga
     2041 ttttgctggc aacgatcgag aggagcagta ggaacgaagc aatcaccatg acaagctcaa
     2101 ttgacggcat tcattcctcc gacgcatatt ggctttttac cataatgaga atggttttgc
     2161 ccggaaaagg gcactatggt ttaaacataa ccacaaaaaa ggatggccgg aagggtcttc
     2221 atgtttcagg acggcgactc ggaccgggag agtgctgatt gcgaacttga aaaggggtgt
     2281 gggacatgct gccaggagcc gggcaaggcg ctttatccgg cggcatgcaa aaggccggat
     2341 gcgtgccggc catgacggaa tgcggtggag gtccgggttt atccctcaat gaaagccaga
     2401 ttctcgccga gtttcctgac ttccccgata ttgtcgagga ggaggctggc aatcctgggg
     2461 tcaaactggc ggccgctctg ctcccgcata taggcttcga cttctcccgg gggaagcgcc
     2521 ttgcggtaga cccggtcact ggagagggca tcaaagacat cggcgacggc cgtgatccgg
     2581 gcgaagaggt cgatggactc gccggccagg ccatgggggt aaccggatcc gtcccagttt
     2641 tcgtggtgct gggccgcgat ctgggcagcg acccggatca ggctccgttt ggaaaagcgc
     2701 aacatctctt cacccgccgg cgcgtgtttc tggatctcgg cgcgttccgt tacggagagc
     2761 ctgtccggct tgaggagaac ggcgtccggg atggcgagtt taccgatgtc gtgcatcgcc
     2821 gaggccaggc gcagggtttc gagatctttt tcgggcaggc ccagttttcg tcctagccag
     2881 acgctgtatt cactgacgcg ctggacatgg tgctgcgttt ccggtgaacg accctcggcg
     2941 atttccccca aggtgaagat gatctctttt tgtgtttctt cgatttcctg gttgaggtag
     3001 atattgtcaa aagcgattgc cacgttggag cagaagatgt cgatcaggtt cttttcccat
     3061 tggtcgaagt cctcggtccg ctcgagatag atgatgttct ctaccccgtt tttgcttctg
     3121 aaaaaaccga tgtagtaatt tttttcggag atggtccgct tttccttgac ggcctgttcg
     3181 atcagaccga agaccgtgtc cgggatgggg gccatttggt cgtttcgggt ttccccgtcc
     3241 tcgtatttgc cggttgcggc caggacc
//
LOCUS       DAOSHE010000231         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_27684_length_3253_cov_3.833646, whole genome shotgun sequence.
ACCESSION   DAOSHE010000231 DAOSHE010000000
VERSION     DAOSHE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27684_length_3253_cov_3.833646"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="PLD82_06945"
     CDS             <1..1808
                     /locus_tag="PLD82_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65156.1"
                     /translation="SLRIEAAAVLPAGMDAAAMERLLRNYCTMGDPELVLGMRMILSA
                     DRGRNSEPNQALGLLLHLLQTASVEAGDTMAIAIALAHAKLYASVNETIRGEIRRDVT
                     RQYALSRRLAAWQDQRFEFRYNISRLPLVPKIYWADRMVYPPEDIRYPVRTLEDYRDY
                     VESIETLEYFHALAERSALYKGATPGDVAKAVYRFVHAKDKNGTKRHLYDPSLEKVRE
                     LFRFYPHDASQGEVGADKRIRYRGISRTASGFIWINYQRTLLERTGHTIGLCEVSSLF
                     ELALFKGMGMASGLMIRFPPKDRPIPGHVFAMYYDPFFQRWNNLEMIKSLKRTFEMEA
                     VIHKPVWHHRLRGLREKVSEKAAAPRFFAVLSHGISRPVFDRMFFSNTEVFEDTMFSE
                     DTLPEKPQDSDGDGIADFEERRLGLDHKRADTARDGVSDLWKLYQGYDPKKSLAAGSL
                     AAPPLDGLGGGYAWPSQTKTVTVRRHTGRKPAEAANEINTISAVRIGERVYLRVTYHN
                     DIQKTKRRSHNLHIENTGGRGRIPGFDILVTPRAAAPANDKTRALSGDGFADLRGSAL
                     REVECMIPLRYCDGARSLRIRVFTALVRGELVLDL"
     gene            2098..>3253
                     /locus_tag="PLD82_06950"
     CDS             2098..>3253
                     /locus_tag="PLD82_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65157.1"
                     /translation="MTNLRAEERGGYLPLAYSFRRYFPETAREKILSLRQEMDALPGD
                     ELEAIGPLLGDLRLDMAQRLFAKLMKAAAEPQDGQDWNAMSRSLEKLFEQYPALLDKK
                     DNASRAEKLLARYAGKTLEVGIIESSTNTDHRTGYSRPLKLQTHGVDSNAPVSVRLSS
                     SNTLRFDGGKVTVELRGSGLFRIEKLEIDPDMNRINVSAHWQAEQFLAILSKAFSLPK
                     AGVLAVSPQVIALRTRIHAQNRFEGVLKEIWAVQTGNEQAVFALAGSLEQIMNDWPEL
                     NSVTRNQAEVERLGSRFPRVRISSVIREPAETLYVSTGFSTPIEVEMHIDNPERVTVY
                     DPVISVRSAGGLVFAGSRKEWQTRITNQQRTISTKLPVTAASSDPGKNRII"
BASE COUNT          739 a          865 c          916 g          733 t
ORIGIN      
        1 ccagtctgcg gatagaggcc gcggctgtcc tgcctgcggg gatggatgct gccgccatgg
       61 aacgtctcct gcggaactat tgcacaatgg gggatcccga gctggtcctt ggcatgcgca
      121 tgatcctgtc ggctgacagg ggcagaaaca gcgaacccaa tcaggctctt ggtcttttgc
      181 tccatctcct gcagacagcc tctgtcgaag ccggggatac catggcgatc gccattgccc
      241 tggcccatgc caaattgtat gcctccgtca acgagacgat ccgcggcgag attcgccggg
      301 acgtgacacg tcagtatgcg ctttcccgca ggctggccgc atggcaggac cagcgtttcg
      361 agtttcgcta taacatttcg cgtctgcccc ttgttcccaa gatctactgg gccgacagga
      421 tggtctatcc tcccgaggac atccgctatc cggtccgcac ccttgaggac tatcgtgact
      481 atgtcgagtc aatcgagacc ctcgagtatt ttcatgctct ggccgagcgc agtgccttgt
      541 acaagggcgc gacacctgga gatgtcgcca aagcggtcta caggtttgtc cacgcaaagg
      601 acaaaaacgg aaccaagcgc cacctgtatg atccctccct cgagaaggtg cgtgagctgt
      661 tccgcttcta tccgcatgat gcttcgcaag gcgaggtcgg ggccgacaag cgcatccgct
      721 accgcgggat cagtcgcacg gccagcgggt ttatctggat caactaccag cgcaccctcc
      781 tcgagcggac aggacatacc atcggattgt gcgaggtatc ctcccttttc gagctggccc
      841 tgttcaaggg gatgggcatg gcctcgggcc tgatgatccg ctttcctccc aaggacaggc
      901 cgatacccgg gcatgtcttt gccatgtatt acgatccatt tttccagcgc tggaacaatc
      961 tggagatgat caagtcactc aaacggacgt tcgaaatgga ggccgtcatc cacaagcccg
     1021 tctggcatca ccggctgcgt ggcctgcgtg aaaaggtcag cgaaaaagca gcggcaccgc
     1081 gcttctttgc cgtcctgagc cacggtattt cgcgcccggt ttttgatcgc atgttttttt
     1141 ccaataccga ggtcttcgag gacaccatgt tcagcgagga cactctgccg gaaaaaccgc
     1201 aggacagcga tggtgacgga atcgccgatt ttgaggagcg caggctgggg cttgatcaca
     1261 aacgggcgga tacggcccgg gacggggtat ccgatctctg gaagctctat caggggtacg
     1321 accccaaaaa gtcccttgcc gccgggtcgc ttgcggcacc cccgcttgac ggattgggtg
     1381 gcggatatgc ctggccgtcg cagacaaaaa cagtgacggt gcgccggcat acgggccgca
     1441 agccggccga agcggccaac gaaatcaata ccatcagtgc cgtccggatt ggtgagcggg
     1501 tatacctgcg cgtgacgtac cacaacgaca tccagaagac aaagcgccgc agccacaatc
     1561 tgcacattga aaatacaggc gggcggggga gaatcccggg gtttgacatt cttgtgacac
     1621 cccgcgctgc ggcaccggca aacgacaaga cgcgggcatt gtccggggac ggctttgctg
     1681 acctgcgcgg gtctgccctg cgtgaggttg agtgcatgat cccgctacgc tattgcgatg
     1741 gcgcacgctc gctgcgtatc cgtgtcttta ccgccctggt tcgcggagaa ctcgtgctgg
     1801 atttgtaata caacagtttc aaatattttt ttgggaacag ttctcaggcc cgcccgcgtc
     1861 ccgcgggaat gggtaaaaat tatattttgc tttttgttca tttgtatgca atatttggac
     1921 gtctgtatcg tataaaacgg aacaggcgtt tcttgaaata tgataaatta tccccgtgag
     1981 gtgctgtata tgcaaatccg gattatgatt gtatggatgc tgttaacgat tcttgctgtt
     2041 tcatgccagt cagtgactga aacgagaagt cagagaattg cgggaataga gagtgacttg
     2101 acgaatctgc gagctgaaga acgtggtgga tatcttcccc tggcgtacag ttttcgtcgg
     2161 tattttcccg aaacggctcg tgaaaagatc ctttcactca gacaggagat ggatgcatta
     2221 ccgggtgatg agcttgaagc catcggaccg cttctcgggg acttgcgact tgatatggcc
     2281 cagcggttgt tcgccaagct gatgaaggct gctgctgaac cgcaggatgg tcaggactgg
     2341 aatgcaatgt cacggagtct tgaaaagttg tttgagcaat atcctgcact gcttgataaa
     2401 aaagacaacg catcacgagc agaaaagctg ctcgcccggt atgcgggcaa gacactggaa
     2461 gtggggatca tcgaatcatc cactaatacc gatcacagga ccgggtattc tcgcccactc
     2521 aagctgcaga cccatggtgt tgattcaaat gcgccagtgt cagtacggct ttcatcgtcc
     2581 aatacattga gatttgatgg gggaaaggta acagtcgagc tgcgtggttc tggactattc
     2641 aggatcgaga aacttgaaat cgacccggac atgaaccgta tcaatgtttc ggcacactgg
     2701 caggccgagc aatttctcgc gatcctctcc aaagcgttct cacttcccaa agcgggagtt
     2761 ctggcagttt cacctcaagt cattgcgctc aggacaagga ttcatgccca gaatcgcttt
     2821 gagggggtac tcaaggaaat ctgggcagtg caaactggaa acgaacaggc ggtgttcgca
     2881 cttgccgggt cactcgaaca gatcatgaat gattggccag agttgaattc cgttacacgg
     2941 aatcaggctg aagtggagcg attgggaagt cgtttcccca gggtccgcat ttcctcagtc
     3001 atcagagagc cggctgagac tctctatgtc agtaccggtt tttcaacacc gattgaggtt
     3061 gaaatgcata ttgacaatcc ggaaagagtg actgtctatg atcccgttat ctccgttcgt
     3121 tcggcgggtg gactcgtgtt tgccggctca cgcaaggagt ggcagacaag gatcaccaat
     3181 cagcagcgaa ccatatcgac aaagctgcct gttacagctg cctcaagcga tcctggaaaa
     3241 aatcgcatca ttg
//
LOCUS       DAOSHE010000232         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_28081_length_3222_cov_2.925797, whole genome shotgun sequence.
ACCESSION   DAOSHE010000232 DAOSHE010000000
VERSION     DAOSHE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28081_length_3222_cov_2.925797"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLD82_06955"
     CDS             complement(<1..448)
                     /locus_tag="PLD82_06955"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG65158.1"
                     /translation="MEDTISMEKAGLRRILRTRRARRTPDELGRAAESIIQTLKATFQ
                     REEPVLLYRALPDEIDLMALAEAWLAEGRTIAFPAVLPNGKMLFREVRNPATDFIPGY
                     RDILQPDTQCSPVPVNGALVLVPGLGFSRQGERLGRGKGYYDRALAD"
     gene            572..1465
                     /locus_tag="PLD82_06960"
     CDS             572..1465
                     /locus_tag="PLD82_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG65159.1"
                     /translation="MYHEDFRIRLHECDAWGHLNHVQYFSLMQQTALAACSAAGWSRL
                     RFQEEGMLWVVRRSEIAYKKEIPFDAVIRIETSITAGERSRCLREYRFFSPEGVVHAE
                     ASSEWVLLDRETKRPRPIPAEMLALLSGGLPLAAMPRTRHEEGDPPSGAWSVSHRVGW
                     RDLDMDWRVNNAVYAAWTEDCGLGILDAYGWPFPRLLENSFAIVARRYGIEYIQPAVP
                     GDEVETATWVSAVRGVSAERSYRVIRKGDGTVLARARALWAWVSLKTGRPVPIPSEFY
                     ADFLKNFAPGVDDGRLERNEP"
     gene            complement(1584..3143)
                     /gene="nadB"
                     /locus_tag="PLD82_06965"
     CDS             complement(1584..3143)
                     /gene="nadB"
                     /locus_tag="PLD82_06965"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOG65160.1"
                     /translation="MREVDVLVIGCGTAGLSAAIYLARAGRQILVLGKSTDPDESNSR
                     YAQGGIIYRSNDDTPEDLAADIMTAGGGMCYPPAVNLVATEGPSVVDDLLIGIAGVPF
                     TRDEAGDLHLTAEGGHHKRRILHREDSTGRQIVFSLLAYASTLPNITIETGKMAIDLI
                     TRDHHSLDPLSRYYRPECLGAYLLDVTTRKIEPVIARAVVLASGGLGQTYLHTTNPAV
                     ATGDGFAMAIRAGAILINMEYTQFHPTTLWHPRANSFLISEAVRGEGAVLVNQAGREF
                     MQRYHEMGSLAPRDVVTRGILDELIESGENCVYLDLSPIPKHEIAVRFPAIHARCLEY
                     GIDITTTPIPVVPAFHFACGGVRINEHGRTNLKRLYAVGEVACSGLHGANRLASSSLL
                     ECCLYGKRAALHIVSHWQDLCSGTLPDIRPWEDAGLETEHDPLLIAQDWATLKHIMWN
                     YVGPVRTTRRLRRAFSELRSLQENIEDFYRNTRLARSIVELRNAVTTGLKIAESAWSN
                     RSSRGCHFRKE"
BASE COUNT          568 a          913 c         1092 g          649 t
ORIGIN      
        1 cgtcggccag tgccctgtcg tagtagccct ttccccggcc gagccgttct ccctgccggg
       61 agaagccgag tcccgggacg aggacgagcg ctccgttgac cggtaccggg gaacactgtg
      121 tgtccggttg aagaatatcg cgatatcctg gaatgaagtc ggtagccgga ttgcggactt
      181 ctctgaagag cattttcccg ttcggaagga cagcgggaaa ggcgatggtc cgtccttccg
      241 caagccatgc ttccgccagt gccatcaggt caatctcgtc gggaagtgcc cggtagagaa
      301 gcacgggttc ctcccgttga aaggttgctt tcagggtctg gatgatgctt tccgcggccc
      361 ggccgagttc atcgggcgtg cgccgggccc gtcttgtgcg caggatccgg cgcagtccgg
      421 ctttctccat gctgatcgtg tcttccatcc gttcaatcta tggaaggaac ggtcgggggg
      481 caaaacccgc gcgagagacc tgattccggt gtggatggct gggccatcct tctcccggtc
      541 ttgctatcat tgaggggagg agtgacctgc catgtaccat gaagacttcc ggatccggct
      601 ccacgagtgc gatgcctggg gacatctcaa ccatgtccag tatttttcgc tgatgcagca
      661 gacggctctt gccgcctgtt cggctgcggg ctggtcccgt ctgcgcttcc aggaagaggg
      721 catgctctgg gtggtccgcc gctccgagat tgcgtacaaa aaggaaatcc cgtttgatgc
      781 cgtgatcagg attgaaacct cgatcaccgc aggtgaacgg tcccgctgcc tgcgggagta
      841 ccggtttttc tcccccgagg gtgtggtcca tgccgaggct tccagcgaat gggtgcttct
      901 ggacagggag acgaagcgtc cccgtccgat tccggcggag atgctggcgc tcctttccgg
      961 tggccttccc ctggcggcca tgcccaggac ccgtcacgag gagggtgacc cgccgtcggg
     1021 ggcatggtct gtctctcacc gggtcgggtg gcgggacctc gacatggatt ggcgggtgaa
     1081 caacgcggtc tatgcagcct ggacggagga ctgtggattg ggtatcctgg atgcgtatgg
     1141 ctggcctttc ccccgcctgc tcgaaaactc gtttgcgatt gtcgcccggc ggtacgggat
     1201 cgagtacatc caaccggcag ttcccgggga cgaggtggag acagcgacct gggtcagtgc
     1261 cgtccgtgga gtttccgccg agaggagcta ccgtgtcatc cggaagggtg acgggaccgt
     1321 tctggcgagg gccagggccc tttgggcatg ggtttccctg aaaacggggc ggccggtccc
     1381 cattccctcc gaattttatg cggattttct caaaaacttc gccccaggtg tggatgatgg
     1441 ccggttggag cggaacgaac cctgacgggg cgggaggtgc tttcgggagc ctgatgtgca
     1501 acaaccggaa ccttggcgga tccttcggga gcagcagcat actgtggcgg atggttggtt
     1561 gaccattcgg ggcaagcccg ggttcactct ttgcggaagt gacagccgcg cgaagagcgg
     1621 tttgaccagg cggactcggc gattttcagt ccggtggtga cggcgttgcg cagctcgaca
     1681 atcgagcggg ccaaccgggt gttcctgtaa aaatcttcga tgttctcctg caggctgcgc
     1741 aactcgctga aggccctccg caggcgacgg gtcgtccgga ccggcccgac ataattccac
     1801 atgatatgtt tgagggtcgc ccagtcctgg gcgatcagaa gcggatcgtg ctcggtctcc
     1861 agcccggcat cctcccaggg acggatgtcg ggcagggtcc cggaacagag atcctgccag
     1921 tgggagacaa tgtgcagggc ggctcgcttt ccgtacagac agcactcaag cagagaggag
     1981 gaagcaagcc ggttggctcc gtgcaggccg ctgcaggcca cctctccgac ggcgtacagg
     2041 cgtttaagat tggtccggcc gtgttcgttg atcctgacgc caccacaggc aaaatggaag
     2101 gcggggacga cggggatcgg agtcgtggtg atgtcgatgc cgtattccag gcaacgggca
     2161 tggatcgcgg gaaaccggac tgcaatctcg tgcttcggga tgggggacag atcgagatag
     2221 acacagtttt cgccgctctc gatcagctcg tcaaggatcc cgcgggtgac aacatcccgc
     2281 ggggcgaggg agcccatctc gtggtagcgc tgcatgaatt cccgaccggc ctggttgacg
     2341 agcacggcac cctcgccacg gacggcctcg gaaatcagaa aactgttggc acgggggtgc
     2401 cagagcgtgg tcgggtggaa ctgggtatac tccatattga tcaggatggc ccctgcacgg
     2461 atggccatcg cgaatccgtc accggtcgcc acggccggat tggtggtgtg gagataggtt
     2521 tgccccagac ctccgctggc aagaacaacg gcccgtgcga tgaccggttc gatcttgcgc
     2581 gtggtcacat ccagaagata ggcaccgagg cactcgggcc ggtaatagcg ggagagcgga
     2641 tcgagggagt ggtgatcccg ggtgatcaga tcaatggcca ttttcccggt ctcgatcgtg
     2701 atgttcggga gagtggaggc gtatgcgagg agggagaaga cgatctgccg gccggtgctg
     2761 tcctctctgt ggagaatcct ccgcttgtga tgcccgcctt cggctgtcag gtgaagatct
     2821 ccggcctcgt ccctggtaaa ggggacaccg gctattccga tcaggagatc atcgacgacc
     2881 gaggggcctt cggtggcgac gagattgacg gccggggggt agcacatccc gccaccggcg
     2941 gtcatgatgt ctgccgcgag atcctccggc gtgtcatcgt tgctgcggta gatgatcccg
     3001 ccttgggcat agcgggaatt gctctcgtcg ggatcggtgc tcttgccgag aaccaggatc
     3061 tgacgtccgg ccctggccag gtagatggcc gctgagagtc cggccgttcc gcagccgatg
     3121 acaaggacat cgacctctct catgggttgg ggccggcgtc ttcggtccgg gcaaagcgtt
     3181 cgagacaggt cagtgcctcc cggatctcgc cgggagtgtc ct
//
LOCUS       DAOSHE010000233         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_28102_length_3220_cov_4.100158, whole genome shotgun sequence.
ACCESSION   DAOSHE010000233 DAOSHE010000000
VERSION     DAOSHE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28102_length_3220_cov_4.100158"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLD82_06970"
     CDS             complement(<1..1206)
                     /locus_tag="PLD82_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65161.1"
                     /translation="MDKSRIFDVVNQRIVIGTISAENLVGKYGSPLFVYLASSIRQRI
                     NILKDAIKYPRLQVSFALRANSSLAILRLLYKEGAVVTAASSGEVMTAFKAGFSPHHI
                     LAIADLLTDSEIEFCHDRNVRLSFGSVDAISRFGARHRGSEVSIRVSPGRVTGGARAW
                     NDVFGFVPERMDAAAQVIDEFSLRVTGVHASDSVETISSSRIIERLDAVFGAAQRFPE
                     VTYINVGGGLGLPVSTSEEELDIYHFGDVLTKRMQAAGNKIPLTLAVEPGRYLVSDAG
                     YLLMRVIAIRMQDGIPVAVTDASTHLLPDIRLQGRPYLIANGSNPDGAEVPVQVAGCA
                     TDSGDFFSAEPVILGEVRVGDILVVSSAGAYAMALAGVFNMRPRPAEVLIEEGRDRLV
                     RKRESIDDIL"
     gene            complement(1199..2755)
                     /locus_tag="PLD82_06975"
     CDS             complement(1199..2755)
                     /locus_tag="PLD82_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65162.1"
                     /translation="MPCPIGVRPLSDDWTISKIMVCKGSVPSGFYLLHARYSHNKHLA
                     KFCGFMYTQSTPRGQIVAEFSIENNYIREQNQIIERLRTDLKRRDDKIAELDRILLMY
                     ERVSELSRSELIEADSTIRAQETVQELAREELLHAHETLEAHERVEELARDERLDADS
                     VIQAHERVEELAREELRSAQETLVAREKVEDLARNERLEADSTIRAQEVVQELARSEL
                     IAARATVNAHETVEDLARHERLDAENTIRAHEAVQELSRNELLEAKKALTAHEEVGNL
                     ARRERMDADSTIRAHEAVEELAREEQLRMEADRDNVMHRLEMVLGELSDANRTIKALE
                     DLDEHQRQSRIQLDRILRAYDAVEELARKERVEAENTIHALESLDEYQRSTRMELDRT
                     IAAHEAVQELSRQERLDSDQGVNGKVRDLMLKLDTAEQTIDALERLDEYQRKNRIAVE
                     KMLEAYDTIGELHRKHRIELDQFIKAHEAVGDMARRERLDAENMLKAYELLNEFAIEE
                     RKKLIEGKDG"
BASE COUNT          665 a          907 c          898 g          750 t
ORIGIN      
        1 caggatatcg tcgatgctct cgcgcttccg gacgagacgg tcacggcctt cctcaatcag
       61 aacttcagca ggcctgggcc gcatattgaa gacccctgcg agcgccatgg cgtaggcacc
      121 ggctgatgag accacaagaa tgtcaccgac ccgcacctcc ccgagaatga ccggttcagc
      181 agaaaaaaaa tccccactgt ctgtggcaca tcccgcgacc tggaccggaa cctcggcgcc
      241 atccggattg ctcccgtttg cgataagata tggacgcccc tgaagccgga tatcgggcag
      301 aagatgggtc gatgcgtccg tgacggcaac cggaataccg tcctgcatcc ggatggcgat
      361 gacccgcatc agaagatagc cagcatccga gacaagatac cggcccggct ctactgccag
      421 cgtgagcgga atcttgtttc cggcagcttg catgcgcttg gtcaaaacat ctccaaaatg
      481 gtagatatcg agttcctctt cggatgtgct gaccggaagc ccgaggccgc cccccacatt
      541 gatgtaggtt acttcaggaa acctctgggc tgccccgaag acggcatcca agcgctcgat
      601 gatccggctt gagctgatcg tctcgacgga atcactggcg tggaccccgg tcaccctcag
      661 gctgaactcg tcgatcacct gggcggctgc atccatccgc tcaggaacaa atccgaaaac
      721 gtcattccat gcccgggctc cgccagttac ccgtcccgga ctgacccgga tcgagacctc
      781 cgagccccgg tggcgcgccc caaaacggga aatcgcatcg acagacccga aggaaagccg
      841 aacattccgg tcatggcaga actcgatttc cgaatctgtc aagagatcgg caatggcgag
      901 gatgtgatgg ggggaaaatc cggccttgaa ggccgtcata acctcaccgg atgaagctgc
      961 tgtgacgaca gcgccctctt tatagaggag acggaggatc gccagtgaag aattggcacg
     1021 aagggcaaaa ctcacctgga gtctgggata tttgatggcg tccttgagga tattgatccg
     1081 ctggcggatg gagctggcga gatagacaaa gagtggggat ccgtattttc cgaccaagtt
     1141 ctcggccgag attgtgccga tcacaatccg ctggttgaca acatcgaaaa tacgggactt
     1201 atccatcctt tccctcaatc agctttttac gctcctcgat ggcaaactcg ttcaagagct
     1261 cgtaggcctt gagcatgttt tcggcatcca gccgttcccg gcgggccata tcacccactg
     1321 cctcatgggc cttgatgaat tggtcgagtt caatcctgtg cttgcgatgc agttcaccga
     1381 tggtatcgta ggcttcaagc atcttctcga cagcgatcct gtttttcctc tggtattcat
     1441 ccagccgttc gagggcatca atcgtctgtt cagcggtatc cagtttgagc atcagatcgc
     1501 ggaccttgcc gttgacaccc tggtcactgt ccagccgttc ctgtcgcgag agctcctgga
     1561 cagcctcatg ggccgcaatc gtccggtcca gctccatccg ggtcgagcgt tggtactcgt
     1621 cgagggattc aagggcatga attgtgtttt ccgcttcgac ccgttccttg cgggccagct
     1681 cttcgacggc gtcgtaggcc cgcaggattc tgtcaagctg gatccgggac tggcgctggt
     1741 gttcatccag gtcctcaagt gccttgatcg tccggttggc atccgagagt tccccgagca
     1801 ccatctcgag tctgtgcatg acattgtccc ggtccgcttc catccggagc tgttcctcgc
     1861 gggccagttc ctcgacagcc tcatgggcgc ggatcgtact gtccgcatcc atccgttcgc
     1921 gacgcgcgag gttgccgacc tcttcatggg ccgtcagggc ctttttggct tcgaggagct
     1981 cgttgcggga gagctcctgg acggcttcgt gggctcggat cgtgttttcg gcatccagtc
     2041 gttcatggcg agccaggtcc tcgaccgttt cgtgggcatt gacggtcgcc cgtgcagcga
     2101 tcagttcgct gcgagccagc tcctgaacga cctcctgggc acgaatggtg gaatcggctt
     2161 cgagccgttc atttcgggcc aggtcttcga ccttctcgcg ggcaaccagg gtttcctggg
     2221 ccgagcggag ttcctcgcga gccagttctt caacccgctc atgggcctgg atcacactgt
     2281 cggcatccag tcgttcatcg cgggccagtt cctcgacccg ttcatgggcc tccagtgtct
     2341 catgggcatg caggagttcc tcacgagcca attcctgaac cgtctcctgg gcgcggatcg
     2401 tcgagtccgc ttcaatcagc tcggagcggg agagttcgga gacacgctca tacatgagga
     2461 ggatgcggtc aagctcggct atcttgtcat cacggcgttt gagatcggtc cgcaagcgtt
     2521 caatgatctg gttctgctcg cggatgtagt tgttttcgat ggaaaattcg gccacgatct
     2581 gcccccttgg cgtgctttga gtgtacatga agccgcagaa cttggcaaga tgcttgttgt
     2641 gggaataccg ggcatgcaag agataaaaac ctgacggaac agatcccttg cagaccataa
     2701 tcttcgagat tgtccagtca tcggagagtg gacggacacc gattggacaa ggcacaggat
     2761 gtcctcacag acatggcgtc tttccggata caggaacaga tactgttttg tttctttttt
     2821 ttgatgaaca gcctcaggcc ctggagtggg ctgtattgga ccaattatgt tttggtattt
     2881 cggatacccc aggatttgag ccatcgacct gattaacggg tatggcaatc tttgtcatcc
     2941 gcggaaggcg cggatatttg gcgcggatat tcgtaggaac agaaacaaaa aacgtttaat
     3001 gcatttttaa ccgccaatac acgcgaatca acacgaatgt ctcttacttg ttttattcgt
     3061 gtttgtaggc gtttattcgc ggtttactat actttgtcct gtttttgatt ttctgctcga
     3121 ctatagtggt ccccttctcg ttcggaccag gtataatgaa cgaggagaga cgaaatggca
     3181 gagcaaagat ggacggcaaa gcgcaagact gagatcatcc
//
LOCUS       DAOSHE010000234         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_28184_length_3215_cov_3.098418, whole genome shotgun sequence.
ACCESSION   DAOSHE010000234 DAOSHE010000000
VERSION     DAOSHE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28184_length_3215_cov_3.098418"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLD82_06980"
     CDS             <1..274
                     /locus_tag="PLD82_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65163.1"
                     /translation="DYSEKLAEAIDIEIKEIVQGCHDQARDLLMKYSKGLAAIANGLL
                     QKEVLDSADLEAIMKAHGIEKMSKAKAVKPVKKAGPGVKKKTGKTR"
     gene            385..1791
                     /locus_tag="PLD82_06985"
     CDS             385..1791
                     /locus_tag="PLD82_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG65164.1"
                     /translation="MKHGIKASVSGIRGVLGESLTPPVVLGYVNAYAGWLGKGTVAVG
                     RDTRSHGTAIQEFVESVLMACGINVVDLGIVPTPTLLHYVRENGLDGGILVTASHNPI
                     EWNALKLVKKGGLFLNQADFESLSKLFSDTVPKGLYKPAAGIGNKQVDDAAPVGHIEA
                     FVLKCNIQNVRLRKFKVAIDPGNGAGARMDREFLVRMGCSVQMVHGEMTGGFERPPEP
                     RPDALGDLCKLVVREHCQIGFAQDPDADRLCVVDETGTPLSEEMTLALSVWSWLMPKS
                     RKEQAERADQAKAPVVANISTSRMVADVARYYGHPFVTAKVGEANVLQTMKACGASIG
                     GEGNGGVILPAINACRDSFVGMFLIMDLVTVMREPLSRVVEIFHEKIAPRYAMLKDKI
                     SFSGDLASGLRRFRDACSKSGKPYEAMDIDGLRIDFEDGWVHLRESNTEPIVRVIAEA
                     GTEARARQLVALARGALE"
     gene            complement(1796..>3215)
                     /locus_tag="PLD82_06990"
     CDS             complement(1796..>3215)
                     /locus_tag="PLD82_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65165.1"
                     /translation="MITIHHRALAVTNEVLLVSSSPVERGIESCVMAASLSAPLYSTT
                     AAPVVLSRVTIQADSTIIRAISIWLDNDDAVFSRESDTLLGMATTSGTETAIQLTRTL
                     TITNRQRRIWVTAVPHSNATVGASITLSITASPLSLSGQVSAVIPLTPVTSPAGLIVQ
                     DTTPPASPADAQRFPGYGTILLRWLVPDSSDISSFRIILSSGALPGGPLDTGAGQVID
                     TAAISQGAYQEQSLKPLNPVTSYTATIWSLDSAGLWSDGVKLAACIPDWYTNSLPPPS
                     GVRVITSPDSWSVSWQALSGVPVTGYRIRIADEESGIVFPLTDLVAGSSLTIKPAHLG
                     TGSLPRRARLSVIAVDANAATSPNDFTTLNEALSRGLAVRTVDAITSGTISVWNTVFR
                     AGDDRARIVLRLTREDQAIVQVLAVHGERLATLVNRRLPPGTHEFFWDGRTGGSPVNP
                     GLYLIHVRAGDSQQVTRVIVRR"
BASE COUNT          686 a          795 c         1114 g          620 t
ORIGIN      
        1 ggattatagt gagaagctgg cagaggcgat cgacatcgag atcaaagaga tcgtgcaggg
       61 ctgccatgat caggcccgtg atctcttgat gaagtacagc aagggtctgg cggcgattgc
      121 caatgggttg ttgcagaagg aagtgctgga cagtgccgac cttgaggcga tcatgaaggc
      181 acacgggatc gagaaaatgt ccaaggcaaa ggcagtcaag cctgtcaaaa aagccggtcc
      241 aggagtcaag aaaaagacgg ggaagacccg ctgaattgcc ggaaaaacgg tgacaaaaag
      301 gctgtcctga catataaggg gcagcctttt caggtttttg cgctacattg aatggcaaag
      361 agcagctatc aagagaggga aaagatgaaa catgggatca aggcatctgt ctcgggtatc
      421 cggggggtgc tgggtgagtc actgacacct ccggttgttt tgggatacgt aaatgcctat
      481 gccggttggc ttggcaaggg gactgtcgcc gtcgggcggg atacccgttc gcacgggaca
      541 gccatccagg aatttgtgga aagcgtgctg atggcgtgcg ggatcaatgt cgtcgatctg
      601 gggatcgtgc cgacaccgac ccttctgcat tacgtgcgcg agaacggtct ggatggcggg
      661 atcctcgtga cggccagcca caacccgatc gagtggaatg cgctcaaact ggtcaaaaaa
      721 ggtgggctct tcctcaacca ggcggatttt gagtcgctga gcaaactctt ttccgatacg
      781 gtgccgaagg gtctctataa accggcggcg gggatcggaa acaagcaggt tgacgatgcg
      841 gccccggttg gtcatatcga agcctttgtc ctcaagtgca atatccagaa tgttcgtttg
      901 cggaagttca aggttgcgat cgatccgggg aacggggccg gcgccagaat ggatcgcgag
      961 tttctggtcc ggatggggtg cagtgtgcag atggtgcacg gcgagatgac gggtggtttt
     1021 gaacgccccc cggagccccg tccggatgcc ttgggcgatc tgtgcaaact cgtggtgcgt
     1081 gaacactgcc agatcgggtt cgcccaggat cccgacgccg accggctctg cgtggtggac
     1141 gaaacgggga caccgctttc tgaggagatg acgctggcac tctctgtctg gtcctggttg
     1201 atgccgaaga gccgcaagga acaagcagag cgggcggacc aggcgaaggc accggttgtg
     1261 gcaaacatct cgacatccag gatggtggca gatgtggctc ggtattacgg gcatcccttt
     1321 gtgacagcca aggtcgggga ggccaatgtc ctgcagacga tgaaggcctg cggggcatcg
     1381 atcgggggtg aaggaaatgg cggggtcatc ctgccggcga tcaatgcctg tcgtgattcc
     1441 tttgtgggga tgtttctcat catggatctg gtgacggtga tgcgcgagcc cctttccagg
     1501 gtcgtcgaga tattccatga gaaaatcgcg ccacgctacg ccatgctcaa ggacaagatt
     1561 tcgttttccg gggatctggc gtccgggttg aggagattcc gggatgcctg ttcaaagagc
     1621 gggaaacctt atgaggcaat ggatatcgac ggtctccgaa tcgattttga ggatggctgg
     1681 gtgcacctgc gcgagtcgaa caccgagccg attgtgcgcg tgattgccga ggcgggaacg
     1741 gaagctcgcg cccggcaatt ggttgcgctt gccaggggtg ctctcgaata gcagactagc
     1801 gccggacgat gacgcgggta acctgttgcg aatccccggc ccggacatgg atgaggtaga
     1861 ggccgggatt gacgggtgat ccgccagtcc ggccgtccca aaagaactcg tgggttcccg
     1921 gggggaggcg gcggttgacc agggtggcga ggcgttcgcc atgaacggcg agaacctgga
     1981 cgatggcctg gtcttctcgt gtgaggcgga ggacgatccg cgcccggtcg tctccggccc
     2041 gaaaaaccgt attccagacg gagatggttc cactggtgat cgcgtcgacg gtccggacgg
     2101 ccagcccccg ggagagggcc tcgttgaggg ttgtaaaatc attggggctt gtcgcagcgt
     2161 tggcatccac ggcgatgacg gagagccgtg cccgccgggg gaggctgcct gttcccaggt
     2221 gtgccggttt gatcgtcaga ctggagccgg cgacaaggtc cgtcaacggg aagacgattc
     2281 cgctctcttc atcagcaata cggatgcgat agcccgtcac aggaacaccg gagagtgcct
     2341 gccagctcac actccaggaa tccggagacg tgatgacacg gacaccggac ggtgggggca
     2401 ggctattggt gtaccagtcc ggaatgcatg ccgctagctt gacaccgtcc gaccagagcc
     2461 cggcactgtc caggctccag atggtcgcgg tatagctcgt tacggggttg agcggcttca
     2521 ggctctgttc ctgatacgca ccctgggaga tcgccgctgt atcgataacc tgtccagccc
     2581 ccgtgtcgag ggggccaccg ggaagtgcgc cacttgagag aatgattctg aaagaagaga
     2641 tgtcactgga gtcgggaacg agccagcgga gaaggatggt gccataaccc ggaaagcgtt
     2701 gcgcgtcagc ggggctggcc gggggggtgg tgtcttgaac gatcagacca gccgggctgg
     2761 tgacgggggt gagcgggata accgcggaaa cctgacccga gagcgagagc ggactggctg
     2821 tgatcgaaag cgtgatgctt gcaccaactg tggcgttgga atggggaaca gccgtcaccc
     2881 agattcggcg ctgtcggttc gtgatggtga gagtgcgggt gagctggatg gcggtttccg
     2941 ttccagaggt cgttgccatg ccgaggagtg tatcgctctc gcgtgaaaaa acggcatcat
     3001 cgttgtcgag ccagatcgag atcgcacgaa tgatggttga atccgcctgg atggtgacac
     3061 gggagagtac aaccggtgcg gccgttgtgg aatagagcgg agcggagaga ctggcggcca
     3121 tgacgcagga ctcgatgccg cgctcgacgg gtgaagagga aaccagaagg acttcgtttg
     3181 tgacagccag tgcccgatga tggattgtga tcatc
//
LOCUS       DAOSHE010000235         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_28838_length_3162_cov_3.029611, whole genome shotgun sequence.
ACCESSION   DAOSHE010000235 DAOSHE010000000
VERSION     DAOSHE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28838_length_3162_cov_3.029611"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..>3162
                     /gene="rpoC"
                     /locus_tag="PLD82_06995"
     CDS             135..>3162
                     /gene="rpoC"
                     /locus_tag="PLD82_06995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG65166.1"
                     /translation="MKNIRDFDRIMIQLASPERILEWSYGEVKKPETINYRSLRPERD
                     GLFCEKIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVSHFKVRRERMGHIDLASPVS
                     HIWYYKSVPSRIGLLLDMSMNDLRSVIYFEKYIVTDPGESSLEPNQLISEEEYLEMKE
                     KHGNNFEAGIGADVVLKLLAAIDLKTLRAELHERMIEKGAKTDPRTLKRLQIVDDFLN
                     SQNKPEWMILTRIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLQELFA
                     PEIIIRNEKRMLQEAVDALFDNSKRKRMVKGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIMKKLNDKGHVYNIKSAKKMVER
                     ESEEVWAVLEEVVREHPVLLNRAPTLHRLGIQAFEPVLVDSKAIKLHPLVCHAFNADF
                     DGDQMAVHVPLSPEAQIECWFLMLSSKNLLNPANGQPIVGPTQDMVLGICYLTKEDSR
                     LRSKTRKLLFSDFHEVMMALEFREIHLQEPIKVRFKGANIVTTPGRIIFNRVLPEDYP
                     FVNYELRKKEISSLIADVFKKYGASSLVDILDSIKNLGFRYATLFGVTIGVDDLLVPD
                     EKFTILDEARKKVENVHEMYRSGHLTNAERYNMIINEWTQANNKISDVLFRRMKEDKN
                     GFNSIYIMADSGARGSQQQIKQLTGMRGLMAKPSGEIIEYPIVSNFKEGLNVLEYFIS
                     THGARKGLADTALKTADAGYLTRRLVDIAQDVMISEMDCGTINGMEMRAIEEGDTVIE
                     TLAERIVGRTATDDVLHPKSGEVIVKANDEITDEQAEFIEKCGIDAVRIRTVLTCESI
                     HGICGKCYGRNLATNCQIELGEAVGTIAAQSIGQPGTQLTMRTFHVGGIAAGQIKDDT
                     LTLKYDAIIKTLPENRIPLENPLNKRLRGITPRKGEMVVQKIVGSYAIAKWQSIKVKD
                     GDMVIAGDPVGIDEDGKEVRAPQHANVSVDKRVRLLL"
BASE COUNT          800 a          790 c          871 g          701 t
ORIGIN      
        1 gatatgcgca tttatgaccg tgaaggtctt gagattgccc tcaccgaaaa ggacgagaag
       61 atcatcgagg ccaaaaaggg cgagaaggct gtaggcgcca actgaccatg cggaattggg
      121 acaaggagta attgatgaaa aatattcgtg attttgacag gatcatgata cagctggcct
      181 caccggagcg cattctcgaa tggtcgtatg gcgaggtgaa aaagcccgag accatcaact
      241 atcgctctct caggccagag cgggatggtc ttttttgtga aaagatcttt ggcactacca
      301 aggagtggga gtgttactgc ggcaaattta aatccatccg ctacaagggt gtgatttgcg
      361 atcggtgcgg tgtcgaggtt tcgcatttca aggttcgtcg cgagcggatg ggccacattg
      421 acctggccag ccccgtttcc catatctggt attacaagtc cgttcccagc aggatcggac
      481 tgctcctcga tatgtccatg aatgacctcc ggtccgtgat ttatttcgaa aaatacattg
      541 tgacggatcc aggtgaatcg agcctcgagc cgaatcaact catctccgag gaagagtacc
      601 tcgagatgaa ggaaaaacac gggaacaact tcgaagccgg gatcggtgcc gatgttgtcc
      661 tcaaactcct tgcggccatc gatctgaaaa cccttcgcgc cgagcttcat gaaagaatga
      721 tcgagaaggg tgccaagacg gacccgagaa cgctcaagcg cttgcagatt gttgatgatt
      781 tcttgaattc gcagaacaag ccggagtgga tgattctgac ccggattccg gtcattcctc
      841 cggagttgcg cccgatggtg cagctggatg gcggccggtt tgcgacttcc gacctgaacg
      901 acctctaccg acgtgtcatc aaccgcaata atcggctcaa gaggttgcag gaactctttg
      961 ctcccgaaat catcatccgc aacgaaaagc ggatgttgca ggaagctgtg gatgcgctct
     1021 ttgacaattc caagcgcaag agaatggtga aggggccggg caatcgcccg ctcaagtcac
     1081 tctcggacat gctcaaggga aagcaggggc gtttcagaca gaacctgctc gggaagcgtg
     1141 tcgattattc aggtcgctcc gtgattgtgg tcgggcctga actcaaactg catcagtgcg
     1201 gtctcccgaa gaaaatggca cttgagctct tcaagccgtt catcatgaaa aagctcaacg
     1261 acaagggcca tgtctacaat atcaaaagtg ccaaaaaaat ggtcgaacgg gagtccgaag
     1321 aggtttgggc ggtcctggaa gaagttgtcc gcgagcatcc agttcttctc aaccgcgcac
     1381 cgaccctgca cagactcggt atccaggcgt tcgagccggt tcttgtcgat tccaaagcca
     1441 tcaagttgca tccgctggtc tgtcatgcct tcaacgcgga ctttgacggc gaccagatgg
     1501 ctgtccacgt tccgctttca cctgaagcgc agatcgagtg ctggttcctg atgctttcat
     1561 ccaaaaacct gctcaacccg gccaacggac agccgatcgt gggaccgacg caggacatgg
     1621 tgcttggtat ctgttacctg acaaaagaag actcgcgatt gcgcagcaag acccgcaagc
     1681 ttctcttctc ggattttcat gaagtgatga tggcgctcga gttcagggag atccaccttc
     1741 aggagccgat caaggtccgc ttcaagggtg ccaatatcgt gactaccccg ggtcggatca
     1801 ttttcaaccg ggtgctgccc gaagactatc cttttgtcaa ttatgaactt cgcaaaaaag
     1861 agatttcttc cttgattgcg gatgtattca agaagtatgg cgcatccagc ctggtggata
     1921 ttcttgacag tatcaagaat ctcggttttc gctatgcgac gctctttgga gtgacgattg
     1981 gcgtggacga ccttcttgtt ccggacgaaa aattcaccat tcttgatgaa gcccggaaaa
     2041 aggtcgagaa cgttcacgaa atgtatcgca gcggccattt gaccaatgct gagcgataca
     2101 acatgatcat caacgaatgg acccaggcca ataacaagat ttcggatgtg ctcttccggc
     2161 ggatgaagga agataaaaac ggattcaact ccatctatat tatggccgac tccggagctc
     2221 gtggcagcca gcaacagatc aagcagttga caggcatgcg cggcttgatg gcaaaacctt
     2281 ccggtgagat catcgagtat ccgatcgtct ccaacttcaa ggaagggctc aatgttctcg
     2341 agtacttcat ctcgacacac ggtgcccgca agggtcttgc cgatacagcc ctcaaaacag
     2401 ccgatgccgg gtatctgacg cgtcgtctcg tggatattgc acaggatgtc atgatcagcg
     2461 agatggattg tgggaccatc aacggcatgg agatgcgggc catcgaagaa ggcgatacag
     2521 tcatcgagac gctggccgag aggattgttg gccggaccgc cactgatgat gtgctccatc
     2581 cgaagtcggg cgaagtgatc gtcaaggcaa acgacgagat cactgatgag caggccgagt
     2641 tcatcgaaaa gtgcggtatt gacgctgtca ggatcaggac agtcttgacc tgtgaatcga
     2701 ttcatggtat ttgtggcaaa tgctacggtc ggaatctcgc gacgaactgc cagattgagc
     2761 taggcgaggc cgtcggaacg attgcggccc agtccatcgg tcagccggga acccagttga
     2821 cgatgcgtac cttccacgtt ggaggtattg cggccggtca gatcaaggat gacacgctca
     2881 cactcaagta cgatgcgatt atcaaaaccc tgcccgagaa taggattccc ctcgagaatc
     2941 ccctcaacaa gcgtttgcgc gggatcacgc cccgcaaggg tgagatggtt gttcaaaaaa
     3001 tcgtcggttc ctatgctatt gcaaaatggc aaagcataaa ggtcaaagat ggtgacatgg
     3061 tcatcgcagg agatccggtc gggattgatg aggacggaaa agaagtccgt gctccccagc
     3121 atgccaatgt ttccgtggac aagagggtcc ggctgctgtt ga
//
LOCUS       DAOSHE010000236         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_29006_length_3149_cov_3.180026, whole genome shotgun sequence.
ACCESSION   DAOSHE010000236 DAOSHE010000000
VERSION     DAOSHE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29006_length_3149_cov_3.180026"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..445
                     /locus_tag="PLD82_07000"
     CDS             23..445
                     /locus_tag="PLD82_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65167.1"
                     /translation="MPVKPAGTVKALLVEDEFSGRMLLQQHLAKFGFEPHIAVNGYEA
                     VGAFGMALESGEPYSLVCLDIMMPDMDGLTALQKIRELEEKRGVISSRGARIIMTTSL
                     SDPANVRTAYGSLCDGYLVKPISRDSIEAELRRLGLVP"
     assembly_gap    720..729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <730..2824
                     /locus_tag="PLD82_07005"
     CDS             <730..2824
                     /locus_tag="PLD82_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65168.1"
                     /translation="IQDALVRTGRSFTTYKYYADAVLKWASNPNNLRSMPGFPRSEDT
                     LKTNFEPLLELLVEKGVLIVGQDPQSDDGRRTISFAFENSIKSNAEAQEHEKIAALLE
                     GEYAKIKAGALLPLPSRRQIEKVLEHEIGPKIRIQPLAPQKYTKRDLAALNAAQTDFF
                     EIDFGNQYLALVPPRFLQDLREIGANRLVGMMQDVALVRRLDAVLAAAVGGEKPMTVT
                     ALFSEKNGRLPAFEKHLAPWLVVIKKILDDVREKEGATPVGRAKFYPVFLLYNWLIQE
                     QREEQRAAVEIRVFETLYAHLVAQKRAVSADEMRYLDAAQAYINEHSLEEYDGLFERF
                     VQGWSLQTNPGKPETIIQIKGSPLGTVYIQSKNGFEIFAAWPVREERHIAEMVESVWV
                     RPLVDRRASPELNQSMNADESFETHFARALHDNFGQDSSFQLFFSLLAVFTRSPAALT
                     SLAGRSGIAAGRFLMQGGDGKLQMRPVSALCGWNRQRILEEVERRIPFMRKLIAAIKG
                     LFGGGGKKETRAGQPKKKGPASHGSESKGQTAEQHKATKDGGAHKKAAKSGDGGTGPD
                     AAAKAKAAQKALPKNLKPVTPDEIQALFSKGKTKSQHLAVWNRQIGQAADENKRIVDE
                     IVAEKLGPIKRKLKAGEKTVEVGLHAYYILNSPRLDGLQNKEALYAYVGLSMIEALEE
                     FRKANRLNFTYLAKK"
     gene            complement(2835..>3149)
                     /locus_tag="PLD82_07010"
     CDS             complement(2835..>3149)
                     /locus_tag="PLD82_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65169.1"
                     /translation="DIRNSCRIQAPDTPLRVGVPTGLTLEVPEAISAQLRRMAWSLIP
                     TNTARIQWPTNSRGDRTSTNDRQATLTALAPGRLEISVFAFYGPQTSPQRIAVRTLDV
                     LP"
BASE COUNT          695 a          889 c         1001 g          554 t
ORIGIN      
        1 caaggccccg gctccaacgg atctgcctgt caagcctgcc gggacggtca aagcgcttct
       61 cgtcgaagac gagttttcgg ggaggatgct cctccagcag caccttgcaa aattcggctt
      121 tgagccccat atcgcggtca acggctacga ggccgtcggt gccttcggca tggcgcttga
      181 gtccggagag ccgtacagcc tggtctgtct ggacatcatg atgccggata tggacggatt
      241 gacggccctc cagaagatcc gggaactcga agagaaacgg ggcgtgatct cctcgcgtgg
      301 agcgcggatc atcatgacaa cctcgctcag tgatccggcc aatgtgcgga cagcctacgg
      361 gagtctctgt gacgggtatc tggtcaaacc gatcagccgg gactcgatcg aggcagagct
      421 gcggcggttg gggctggtgc cctgagagga gagccgggaa cggatgcggc ctgcgcgtat
      481 tgcccggctt gcactcctgc ctgatcctgc atatacttga aaccggaatg cctcaagcgc
      541 aggacaacgg gaagaggtgg caggcgtggc ccatcaggaa acagcggcgg agcaactcga
      601 acgatacaag acgatgccag cctttgcccg ggatttcagg aacaaagcgc ttgaccagca
      661 gacagaggtc tatcaggaat tcgggatcaa acatgcggtc caccgcaaga tgttcgagtn
      721 nnnnnnnnng atccaggatg cccttgtccg gacgggccgt tcctttacga cgtataaata
      781 ttatgcggat gcggtcctca aatgggcctc caaccccaac aacctgagga gcatgcccgg
      841 tttcccccgg agcgaggaca ccctcaagac caatttcgag cccctgcttg aactccttgt
      901 cgagaagggc gtgttgatcg tcgggcagga cccccagtcg gatgacggac ggcggacgat
      961 ctccttcgcg ttcgaaaaca gcatcaagtc caatgccgag gcacaggagc acgaaaagat
     1021 tgcggccctg ctcgaggggg agtatgccaa gatcaaggcc ggggccctgc tgcccctccc
     1081 gtcgcggagg cagatcgaaa aagtccttga gcacgagatc ggtcccaaga tccgtatcca
     1141 gccgttggct ccgcaaaagt ataccaaacg ggatctggcg gcactcaatg ccgcgcagac
     1201 ggattttttc gagatcgatt tcgggaacca gtatctggca ctggtgcctc cgcgctttct
     1261 ccaggatctg cgggagatcg gggccaaccg actggtgggg atgatgcagg atgtcgcgct
     1321 cgtgcgcagg ctggatgcgg tcctggccgc agcggtcggg ggcgaaaagc cgatgacggt
     1381 gaccgccctc ttttcggaaa aaaacggacg gctgccggcg tttgaaaaac accttgcccc
     1441 ttggctggtg gtgatcaaaa aaatcctcga tgacgtccgg gagaaggaag gagcgacccc
     1501 tgtcggcagg gccaagttct atccggtctt tctgctgtac aactggctga tccaggagca
     1561 acgtgaggaa caacgggctg ccgtcgagat ccgggttttc gaaacactct atgcccacct
     1621 tgtcgcccaa aagcgcgcgg tatcagcgga cgagatgcgc tatctggatg ctgcgcaggc
     1681 ctatatcaac gagcactctc tcgaggaata cgacggactc ttcgagcgct ttgtccaggg
     1741 ctggtcgctt caaaccaatc ccggcaagcc ggagaccatc atccagatca aggggagccc
     1801 gctggggacg gtgtacatcc agtcgaaaaa tggattcgaa attttcgccg cctggccggt
     1861 gcgtgaggaa cgccatattg ccgagatggt cgaatcggtt tgggtgcgcc ccctggtgga
     1921 ccggcgcgcc tccccggagc tcaaccagtc gatgaatgcg gatgaatcct tcgagacgca
     1981 ctttgccaga gccctgcacg acaacttcgg gcaggacagc tcgttccagc tctttttctc
     2041 gctgctggcg gtgtttacca ggagtccggc cgcactgacc tcgcttgccg gtcggagcgg
     2101 gattgcggcc ggccggtttt tgatgcaggg cggggacggc aagctccaga tgcggccggt
     2161 ctcggcgctc tgcggatgga acaggcagcg aatcctcgag gaagtcgaac ggcggattcc
     2221 cttcatgcgc aagctgatcg cggccatcaa gggcctcttt ggcgggggcg gaaaaaaaga
     2281 gacgcgcgcc ggacaaccga aaaaaaaggg tccggccagc cacggaagcg agagcaaggg
     2341 ccagacagcg gagcaacaca aggccacgaa ggacgggggc gcgcacaaga aggctgccaa
     2401 gagcggagat ggcgggactg gcccggacgc ggccgccaag gccaaggccg cacaaaaggc
     2461 tcttcccaaa aacctcaagc cggtcacacc ggatgaaatc caagcgcttt tttcaaaggg
     2521 caagaccaag tcacagcatc ttgccgtctg gaaccgacag atcggtcagg cggccgacga
     2581 aaacaagcgg atagtggacg agatcgtcgc ggaaaaactc ggtccgatca agcgcaagct
     2641 caaggccggg gagaagaccg tcgaggtcgg actgcatgcc tattatatcc tcaacagtcc
     2701 gagactggac ggcctgcaaa acaaggaagc cctctacgcc tatgtcggtc tctcgatgat
     2761 cgaggcgctt gaggagttcc gcaaagcaaa ccggctcaat tttacctatc tggcaaagaa
     2821 atagggctgc ctggtcaggg gagcacatcc agggtgcgga ccgcaatccg ctggggactg
     2881 gtctgtggac catagaacgc aaacacagag atttcgagac ggccgggagc cagggcggtc
     2941 agggtagcct gccggtcgtt ggtgcttgtc cgatctccgc gggaattggt tggccactgg
     3001 atgcgggccg tgtttgtggg gatgagactc cacgccatcc ggcggagttg tgccgagatg
     3061 gcctccggga cctcgagggt cagtccggtc gggacaccga ccctgagcgg ggtgtccggt
     3121 gcctggattc ggcaggaatt gcggatatc
//
LOCUS       DAOSHE010000237         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_29013_length_3148_cov_4.928225, whole genome shotgun sequence.
ACCESSION   DAOSHE010000237 DAOSHE010000000
VERSION     DAOSHE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29013_length_3148_cov_4.928225"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLD82_07015"
     CDS             complement(<1..874)
                     /locus_tag="PLD82_07015"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65170.1"
                     /translation="MFEQHIERLTRHQAMDEVVARASSASSVRCGALWGASRALVAFA
                     LARRTGRPLLLVTATSHEAEDLHGDLLAFSGEGAGVHLFPHTEILPYEDVSPFSDIVH
                     HRISVLHALVDGSAKVVVAPVRAFLARLIPRASFTGAQLLLRKGDLYELQDLVRYLVN
                     LGYEQSSRVERAGEFAVKGGILDIFPSNLQDPCRIEFFDIEIESLRSFDAGSQRSLGP
                     VESLLILPQREIMLHDENRTRALSEVVRIFPPSEDRDGLGDLLAAGVDFPGMENYLPL
                     FFEETSTLEAFFPER"
     gene            complement(915..1460)
                     /locus_tag="PLD82_07020"
     CDS             complement(915..1460)
                     /locus_tag="PLD82_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65171.1"
                     /translation="MAEVNSRIVEFFVSQLGGNASLEKYRKQGEWASKDVIPAFTKAL
                     LAKGNRMQLKHAGRAFSAIVMEDLDSMYEYATVQEAIEFLPKIFTHYLHGEGSGIWKT
                     DNISPGFVCLRENTPFDCFFTEGLLLGLLQNLGAAGAVVHQTDCREENPRAKFCVYEL
                     KWMRSNLARPAGMQRDPKRPR"
     gene            complement(1453..>3148)
                     /locus_tag="PLD82_07025"
     CDS             complement(1453..>3148)
                     /locus_tag="PLD82_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOG65172.1"
                     /translation="LAAMISENDARPRFRLANYGQYLEKHPPQGEVRLKDGNAGLGTA
                     WSCAHGVGRWMDDCGCGGGGPGGWHQRWRAPLRAAFDVLRDQIWKVTMETVSGMIADI
                     EAARNDYIQVIRSGEQVAAGKISEELHHGVFDAFVKRHALRELTEPEKRQLRALFEAQ
                     RYALYMYTSCGWFFAEISGLEGVQCMRYAARAIDLAEEWLPEDIEEQVLAVLETAPSN
                     VGHFGNGRRVWNELVRKHVLDNERVINQFVLRHLFAGGPESGKMFYYSIALDGVEETR
                     SADGAFLFSGRARLEDGMSWSESSWFWFVRFMPPAGITFWTRSSADARLGSWLREVFR
                     TSLPAELQRHMKEWIPRALGIADLKFEERALLVEALFRDRIEGLERPDAASRLDSILE
                     MAAVFGFHGVPLPEDVRPAIEMLCNSIIRRELVRLSAGDESCDYALVARIFESARNTG
                     LSINKKAVAVIYARQLVERYEAFASRPDDDPVWAALVTVLEFANRAGLEYDRRWIENR
                     AWKDLHEIVGPAISRAKAEESTMVQRYLAAAEQVNLQIAPWRQRAREAFEQEDGNG"
BASE COUNT          763 a          983 c          807 g          595 t
ORIGIN      
        1 gccgttcggg gaaaaacgcc tccagcgtcg atgtctcctc aaaaaacagc ggaagatagt
       61 tttccatccc gggaaaatcg acgccggcag caagcagatc tccaagcccg tcccggtcct
      121 cggaaggagg gaaaatgcgg acgacttcgg agagtgcccg cgtcctgttt tcatcatgaa
      181 gcatgatctc ccgctgggga agtatcagga gtgactcaac gggcccgagg gaccgctggc
      241 tgccggcatc aaaactccgc aaagactcga tctcgatatc gaaaaactcg atccggcagg
      301 gatcctggag attggacggg aagatgtcga ggatcccgcc cttgacggcg aattcgcccg
      361 cacgttccac tctcgaggat tgttcatacc cgagattcac aagataccgc acaagatcct
      421 gaagttcgta caggtcccct ttgcgcagga gaagttgggc gccggtgaaa ctggctcggg
      481 gaatcaagcg agccagaaag gcccggacgg gggcgacaac caccttggcc gaaccatcca
      541 ccagtgcatg caggacagaa atccggtgat gcacaatatc ggaaaatggt gatacatcct
      601 cgtacggaag aatctccgta tggggaaaaa gatggacgcc ggctccctct ccggagaacg
      661 ccagaagatc accatggaga tcttcggctt catgggacgt cgccgtcacc aacagaaggg
      721 gccgtcccgt acggcgcgcc aaagcaaacg cgacaagcgc ccgcgacgct ccccagagag
      781 ccccgcagcg gacggaggaa gcactgcttg cccgcgctac aacctcgtcc atcgcctgat
      841 gccgggtcag gcgctcgata tgctgttcga acatcccaca aatatagcgg aggcggaaat
      901 aaaaaagacc cgcatcaacg cgggcgcttt gggtctctct gcatcccggc tgggcgggcg
      961 agatttgatc gcatccattt cagttcatag acacagaact ttgcgcgggg attctcttcc
     1021 cggcaatccg tctgatgcac gacagcccct gcagctccaa gattctgcaa caaccccaga
     1081 agcaggcctt ccgtgaagaa acagtcaaaa ggagtgttct cccgcaggca gacaaagccc
     1141 ggggagatat tgtcggtctt ccatatgccg gacccttcac catgcaggta gtgggtgaaa
     1201 atctttggca aaaactcgat cgcctcctgg accgtggcat attcatacat cgaatccagg
     1261 tcctccatga cgatggccga aaacgccctt ccggcatgtt tgagttgcat gcgattccct
     1321 ttggcgagaa gcgctttggt gaacgcggga atgacgtcct tggatgccca ttcaccctgc
     1381 ttgcggtatt tctcgagcga ggcattgcca ccaagctgac taacaaaaaa ttcgacgatc
     1441 ctcgaattga cctcagccat tcccgtcctc ctgttcaaac gcttcccggg cccgctgccg
     1501 ccatggtgca atctgcagat tgacctgttc ggctgctgca agatagcgct gcaccatggt
     1561 gctctcctct gccttggcgc gactgatggc cggccccaca atctcgtgca ggtctttcca
     1621 agcccgattc tcgatccagc gccggtcata ctccagcccg gcccgattgg caaattccag
     1681 taccgtgaca agtgcggccc agacgggatc atcatccggc cgggatgcaa aagcctcata
     1741 tctctcgaca agctggcgtg catagatgac agcaaccgcc ttcttgttga tgctcaaccc
     1801 cgtattccgg gccgactcga aaatacgggc gacaagagca taatcacagg actcgtcacc
     1861 ggcagaaagc ctgacaagct cccgtcgaat gatggaatta cagagcattt caatcgcggg
     1921 acgaacatcc tcggggagcg gaacaccatg gaagccaaag acagccgcca tctcgagaat
     1981 gctgtcgagt ctcgatgccg catcaggtcg ctcaagtccc tctatccggt cccgaaagag
     2041 cgcctccaca agaagtgccc gttcttcaaa cttcagatcg gcgatgccca gggcccgggg
     2101 aatccattct ttcatgtgac gctgcaactc tgccggcaag gaagtgcgaa aaacttcgcg
     2161 aagccagctt cccagccgcg catccgctga agaccgggtc cagaacgtga tcccggccgg
     2221 tggcatgaac cggacgaacc agaaccaact cgattcggac cagctcatcc cgtcctcaag
     2281 acgggctctc ccgctgaaaa gaaatgctcc gtcagcagat ctggtctctt caacaccatc
     2341 caaggcaatg ctgtaataaa acatctttcc tgactcgggc cctccggcaa aaagatgccg
     2401 caaaacgaac tggttgatca cacgctcatt atcaagaaca tgtttacgaa caagctcatt
     2461 ccagacacgg cgaccattcc cgaagtgacc aacattggaa ggtgctgtct ccaaaactgc
     2521 caggacctgc tcctcaatat cctcgggcaa ccattcctcg gcaagatcaa tggcccgggc
     2581 cgcatagcgc atgcattgca caccctccag cccggaaatt tctgcaaaaa accatccaca
     2641 actcgtatac atgtacagag cataccgttg cgcctcaaag agcgcccgca actgacgctt
     2701 ctccggctcc gtgagctcac ggagcgcatg acgcttgaca aacgcgtcaa agacgccatg
     2761 gtgcagttct tcactgattt tcccggctgc aacctgctca ccggaccgaa tgacctggat
     2821 atagtcattt ctcgccgcct caatgtcggc aatcatcccg gagaccgtct ccatggtgac
     2881 tttccagatc tggtcacgca gaacatcaaa agcagccctg aggggggcgc gccaacgctg
     2941 atgccatcca ccgggcccac caccaccaca cccacagtca tccatccagc ggccgacacc
     3001 atgggcacag ctccaggccg tccccaatcc agcattccca tccttgaggc gtacctctcc
     3061 ctgcgggggg tgcttttcaa ggtattgtcc ataattcgca agcctgaacc gggggcgggc
     3121 atcattttcc gagatcatgg ctgccagg
//
LOCUS       DAOSHE010000238         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_29275_length_3129_cov_3.661028, whole genome shotgun sequence.
ACCESSION   DAOSHE010000238 DAOSHE010000000
VERSION     DAOSHE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29275_length_3129_cov_3.661028"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..869)
                     /gene="rsmI"
                     /locus_tag="PLD82_07030"
     CDS             complement(168..869)
                     /gene="rsmI"
                     /locus_tag="PLD82_07030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG65173.1"
                     /translation="MKNGCLYIAATPIGNLEDLTLRTIRLLKEADLVICEDTRRTLAL
                     LNHLQIKKELDSCHAHTPQGRILKMASRIEDEDLTAVLVTDGGTPGISDPGAALVRAC
                     RERSIPVFPLPGPSALASALSVSGFPSNNLVFTGFLPLKPGKRRKALLAALDNYATVV
                     CYESPWRVDALLDDIAAVAPGVQVLVAREMTKLHEEYILWIAGTDKPELTRKGEFTLV
                     IHAGERIPDQDENIQ"
     gene            complement(866..1717)
                     /gene="folD"
                     /locus_tag="PLD82_07035"
     CDS             complement(866..1717)
                     /gene="folD"
                     /locus_tag="PLD82_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG65174.1"
                     /translation="MTARILDGKIPASRIFDQIRARVSASSITPGLAVIQVGSDPAST
                     VYVNMKEKRCSECGILSRVFRLPDSTAQEDLLDLVRQLNADPAIHGILVQSPLPGHID
                     EFAVIETVSPLKDADCFHPCNIGRLVAGAPLVHPCTPAGALHILRYYGIDPAGKRALV
                     VGRSNIVGKPLGLMLLAENASVGWAHSKTRNLASLCREAEILCVAVGKPGCITADMVS
                     EGAIVVDVGINRVARGESSVLVGDVDFEGVGQKAAWITPVPGGVGAMTIAMLLSNCLR
                     LAGIPLP"
     gene            complement(1734..2945)
                     /locus_tag="PLD82_07040"
     CDS             complement(1734..2945)
                     /locus_tag="PLD82_07040"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG65175.1"
                     /translation="MYILVLNSGSSSVKYQLVSITDESIVTRGCVEKIGSKEAIHTYS
                     AGEQGKLKEVHPIVNHEEALSVVFRALTNPAHPVIHDLKQIAAVGHRVVHGGAKFTET
                     VLVTEEVIKDIRECIELGPLHNPHNLKGIDVCMRLLPGTPQLAVFDTAFHQTMPEHAY
                     LYALPYVFYTRHRVRRYGFHGTSHRFITERASVLLNKAQKNVNLVSCHLGNGSSICAI
                     RGGKSIDTSMGFTPLEGLIMGTRSGDMDPAIIQYIMHKENYTIQEVDSLLNKQSGLLG
                     ISGATNDLRELFSLADKKDQRAQLAIQMMVYRLKKYIASYMAVLNGEADAVIFTAGIG
                     ENNAWVREQAVEGLEFMGIKIDKKKNKALDGEGDVAASDSRVRILVIPTNEELLIAKD
                     AAAFVRKRRGS"
BASE COUNT          712 a          893 c          823 g          701 t
ORIGIN      
        1 tcaccggaaa tgcgagtcgg ctcgaccgaa gcacttccgg ccttccgcac gctctgtgga
       61 ccggatatcc tcttgggtcc atgaagatca ccaattccgt ctatactcat ggcgaactcc
      121 tttctctctc cagagtatcg gagcaactgg ggaaaaagtt aagcaattta ttggatattt
      181 tcatcttgat cgggaatcct ttccccggca tggatgacca gcgtgaactc cccttttcgt
      241 gtcagttccg gcttgtctgt ccccgcaatc cagaggatgt actcttcgtg cagctttgtc
      301 atctcgcggg ccacaaggac ctggaccccg ggggcgacgg ctgcaatatc atcgagcagg
      361 gcatccacac gccatggcga ctcatagcag accacggttg catagttatc cagagccgca
      421 agcagtgctt tgcgccgctt ccccggtttc aatggtaaaa aaccggtaaa cacaagatta
      481 ttggaaggaa aacctgaaac cgagagagcc gaggccagcg ccgacggtcc gggaagcgga
      541 aaaacaggga tcgaccgttc gcgacaggcc cgcaccagcg cggcacccgg atcggagatg
      601 cccggcgtcc cgccatccgt caccaaaacg gcggtcaagt cctcatcttc aatgcgcgaa
      661 gccattttga ggatcctgcc ctgcggcgta tgtgcgtgac aggaatcgag ctcctttttg
      721 atttggagat ggttcaggag cgcaagcgtc cttcgggtat cctcgcagat cacaagatca
      781 gcctccttga ggagccgaat cgttcgcagg gtcagatcct caagatttcc gatgggtgtg
      841 gccgcgatgt aaagacaacc gtttttcatg gcagagggat cccggcaaga cgcaggcaat
      901 tcgacagaag catggcaatg gtcatcgcac cgacaccacc cgggaccggt gtgatccagg
      961 cggctttttg cccaacgccc tcaaagtcga catcaccaac caggacggaa ctctctcccc
     1021 gggcaacacg gttgatcccg acatcaacca caatggcacc ttccgaaacc atatccgcgg
     1081 tgatgcatcc gggtttgcca acagcgacgc agaggatttc ggcttcccgg cagagtgagg
     1141 ccagatttct cgttttggaa tgcgcccatc ccacactggc attttcggcc aagagcatca
     1201 gcccaagggg tttcccgaca atattgctcc ggccgacgac caaggctctt ttcccggccg
     1261 gatcgatccc gtagtacctg agaatatgga gcgccccggc cggagtgcag ggatgcacca
     1321 agggggcccc tgccaccagt cgcccgatat tgcagggatg aaaacaatca gcatccttga
     1381 gcggactgac ggtttcaatc accgcaaact cgtcgatgtg tccgggaaga ggtgattgca
     1441 caaggattcc gtgaatggca ggatcagcat tcaattggcg gaccaagtcg aggagatcct
     1501 cctgcgctgt cgaatcgggc agcctgaaca ccctcgagag aatcccgcac tcggaacaac
     1561 gcttttcctt catgttgacg tagaccgtcg aagcaggatc actcccgacc tgaataacgg
     1621 ccagaccggg cgtgatggag gaggcactga ctcgtgcccg gatctgatcg aagattcggg
     1681 aagctggaat cttcccgtcc agtattctgg ctgtcatgga ctctcccgga atctcaggat
     1741 ccgcgacgtt tgcggacaaa ggcggctgca tcttttgcta tcaagagctc ttcattggtg
     1801 gggatgacaa gaatccgcac acgcgagtcg gatgcagcaa catcaccctc tccgtcgagc
     1861 gccttgtttt tctttttatc gatcttgatt cccatgaatt cgagaccttc gacagcctgc
     1921 tcacggaccc aggcattgtt ttccccgatc cctgcggtaa agatgacggc atcagcctct
     1981 ccattcagca cggccatgta cgaagcgatg tattttttca accggtagac catcatctgg
     2041 atcgccaact gcgcacgctg gtcttttttg tcggccaggc tgaacagttc ccggaggtca
     2101 ttggttgcac ccgatatccc caacaatccg ctctgtttgt tcaaaaggga atcaacttcc
     2161 tggatggtat agttttcctt gtgcatgata tactgaatga tcgccggatc catatcgccc
     2221 gaacgtgttc ccatgatcaa accttcgagg ggtgtaaacc ccatcgaagt atcgatgctc
     2281 tttcctcccc ggatggcaca gatgctggat ccgttcccca gatggcaact gacgaggttg
     2341 acgttctttt gggccttgtt gagaaggacg gatgcgcgct ccgtaatgaa cctgtgcgag
     2401 gtcccgtgaa aaccatatcg tctgacacga tgcctggtat agaagacata gggcaacgcg
     2461 tagaggtagg catgttccgg cattgtctgg tgaaaagccg tgtcaaagac ggccaactgc
     2521 ggtgttcccg ggagcagccg catgcagaca tcgatgccct tgaggttgtg gggattgtgc
     2581 aaaggtccaa gctcgataca ttcccggatg tccttgatca cttcctcggt cacaagcacg
     2641 gtctcggtga atttggcacc accatgcacc acacggtgcc cgacagcagc aatttgtttg
     2701 agatcgtgaa tcacgggatg cgcaggattg gtcaatgccc tgaagacgac ggaaagagcc
     2761 tcctcgtgat tcacgatcgg atgcacttcc ttgagcttcc cttgctcccc ggcactgtag
     2821 gtatgaatcg cttccttgct cccgattttt tcgacacatc cccgggtcac aatcgattca
     2881 tccgtgatgg atacgagttg atacttgacc gatgaagaac cgctgttcaa aacaagaatg
     2941 tacatatccg acctctttcg tctcgtgcag gatctcttcc cgcgaaatga cattcgaaac
     3001 tctccgccga ttctgcacgg tcctacttga tgggatgtgc aaaaatcata cggccggccg
     3061 gggtctggaa tacgctcgta accatgacag aaatccgctg gttgatgtac ccgcggcctt
     3121 tatcgacaa
//
LOCUS       DAOSHE010000239         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_29382_length_3121_cov_3.469341, whole genome shotgun sequence.
ACCESSION   DAOSHE010000239 DAOSHE010000000
VERSION     DAOSHE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29382_length_3121_cov_3.469341"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="zwf"
                     /locus_tag="PLD82_07045"
     CDS             <1..1011
                     /gene="zwf"
                     /locus_tag="PLD82_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOG65176.1"
                     /translation="CREDQIYRIDHYLGKETVQNILAFRFSNAIFESVWNRNYIDWVE
                     ITAAEELGVGKRGGYYDTAGALRDMVQNHMMQLLALVAMEPPVNFNPESLRNETQKVF
                     QSLRPFEHSDLVDPLPSSEEARGPGRPACIRARYTGYTAESGIAPDSMTETFVAARIF
                     IDNWRWGGVPFIVRTGKKLPVRVTEIVVHFKKTPHILFRNAGTPRVSNSLIIRIQPDE
                     GLLLTLGLKSPGGGFDLRQVPLHFHYRELEGVPLSEAYERLILDCINGDPSLFIRGDA
                     VLACWRFVQPVLEAWRAHAAPLAEYAQGSWGPELADLLLPPGERWREPCRILSEDGVC
                     HL"
     gene            1086..1826
                     /gene="pgl"
                     /locus_tag="PLD82_07050"
     CDS             1086..1826
                     /gene="pgl"
                     /locus_tag="PLD82_07050"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOG65177.1"
                     /translation="MDDERRVFASADDLAISVAALIGERLAGAGARDVHLAISGGKTP
                     GIIFECLADQLPVVLSCRLHVWWVDERLVPPDDPESNYGLFARTFLVRHPLPAGQVHR
                     IHGELDPDRALAGLREELRILAEEECRDAWGCQPVFDLVLLGVGADGHTASVFPGDEA
                     AFEADESCLAVCHPASGQGRITLSPKTIMAAREVVYCLTGTDKTGIAARILDRGAPQD
                     LPAGRIRSRTGVTRWYLDRDAARLLSAP"
     gene            1893..2777
                     /gene="nudC"
                     /locus_tag="PLD82_07055"
     CDS             1893..2777
                     /gene="nudC"
                     /locus_tag="PLD82_07055"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOG65178.1"
                     /translation="MAFQPLARMPDCRNGVTGPAYWICFRGDSVVMKEDGSLPFVEDG
                     AAFLSRLERPVFPCGSLDGIPCYAVDLGEAAPGEAPGDRTAPLLDSLPDEGAPVFLSL
                     RQSLTLTGGGPVWQALGTARHLVEWRRTSRFCGVCGTPLEMNPDEQVFACRACGYQAW
                     PRVTPAVIVAVRDGDRLLLGHNAGFPDGLYAPFAGFVEPGESLEEACSREVREESAVE
                     IGELRYFSSQHWPFPGSLMIAFTAEYRGGEARADGVELTDVRWFTRDDPPPAIPRNGG
                     MGRALYDWFSLGGSVPNG"
     gene            2770..>3121
                     /locus_tag="PLD82_07060"
     CDS             2770..>3121
                     /locus_tag="PLD82_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG65179.1"
                     /translation="MDEKGIGRDGGRWRASPLVRLAAGGLLIGLSAVFVRLVRDDDPG
                     TGPVAVAFWRMLFGGLVLIPLVLKSGRLRPAGLREGSLLFGAALLFACDLTLWHASIH
                     RVGPGLATILGNLQA"
BASE COUNT          502 a          908 c         1060 g          651 t
ORIGIN      
        1 tgccgcgagg accagatcta ccgcatcgat cactaccttg ggaaggaaac cgtgcagaac
       61 atcctcgcat tccggttttc gaatgccatt ttcgaatctg tctggaacag gaattacatc
      121 gactgggtgg agatcacggc ggccgaagag ctcggggtgg gcaagcgggg agggtattac
      181 gacacggccg gtgccctgcg ggacatggtg cagaaccaca tgatgcagct cttggccctg
      241 gtcgcgatgg agccaccggt caatttcaat ccggagtccc tgcggaacga gacccaaaaa
      301 gtctttcaga gcctgcgtcc ttttgagcac tcggatctgg tggatcctct cccctcatcg
      361 gaggaagccc gtgggccggg acgtccggcc tgcatccggg cgcgctatac cggatatacg
      421 gcggagtcgg ggattgctcc cgattcgatg accgagacct ttgtcgccgc acggatcttc
      481 atcgacaact ggcgctgggg aggggtgccc ttcatcgtca ggaccgggaa aaagcttccg
      541 gtccgtgtca ccgagatcgt ggtgcacttc aagaagacgc cgcacatcct cttccgcaat
      601 gcggggacgc cccgtgtttc caacagtctg atcatcagga tccagcccga cgaaggcctg
      661 ctgctcaccc tcgggctcaa gtctcccggt ggcgggttcg atctccggca ggttccgctg
      721 cacttccact acagggaact ggagggtgta ccgctctccg aagcctatga gcggctgatc
      781 ctggactgca tcaacgggga tccctcgctc ttcatccgcg gagatgccgt tctggcctgc
      841 tggcggtttg tccaaccggt ccttgaggcg tggagggcgc atgcagcccc gttggcggag
      901 tatgcccaag ggagttgggg cccggaactt gcggatcttc ttctccctcc cggggagcgt
      961 tggcgcgagc catgccggat cctctcggag gatggggtct gccatctgta gtcctgtttt
     1021 ggtgaacaac ctcgggccgg atatggatgc cgggttctcg gggaaaatgg cagggaggtg
     1081 tggtgatgga tgacgagcgg cgggtttttg catcagcgga tgatctggca atctcggtgg
     1141 cggcgctgat cggggaacgt cttgcagggg ccggggcgcg ggatgtccac ctggcgatct
     1201 ccgggggaaa gacacccggc atcatcttcg aatgcctggc cgaccaactt cccgttgttc
     1261 tgtcctgccg gctccacgtc tggtgggtgg atgagcgtct ggtgccaccc gacgatcccg
     1321 agagcaatta cggactcttt gcacggacct tcctcgtgcg ccatcctctt ccggcaggac
     1381 aggtgcaccg gatccacggt gagctggatc ccgaccgggc cttggccggc ctgcgggaag
     1441 aactccgtat cctggccgag gaggagtgcc gggatgcctg gggctgccag cccgtcttcg
     1501 atcttgtact gctcggagtc ggcgcggatg gacacaccgc ctcggtcttt cccggggacg
     1561 aagcggcctt cgaggcagac gagtcatgcc tggcggtgtg ccatccggca agcggacagg
     1621 gacggatcac gctttctcca aagaccatca tggcggcccg tgaggtcgtc tattgcctga
     1681 cagggaccga caagaccggg attgcggccc ggattctgga tcggggcgcg ccgcaggatc
     1741 tgccggccgg acggattcgt tcccggacag gggtgacccg ttggtatctt gacagggatg
     1801 ctgctcgcct gctttccgcg ccatgagacg ataatccatt tgaggcggtt ttccggatgc
     1861 ggaggggaat gcagcgggcg ggaggagatg tgatggcgtt tcaacctctg gcgcgaatgc
     1921 ctgactgccg gaatggcgtg accgggcccg cgtattggat ctgtttccgc ggcgattctg
     1981 tcgtgatgaa ggaggacggc agccttccat tcgtggagga tggggcggcc tttctgtccc
     2041 gtctggaacg ccccgtgttc ccgtgtggtt ctcttgacgg gatcccctgc tacgccgtcg
     2101 atctcggaga ggctgcgccg ggcgaggctc ctggggatcg aaccgctccg ctgcttgaca
     2161 gcctcccgga tgagggcgca ccggtcttcc tctctctgag acagagcttg acactgacgg
     2221 ggggtgggcc agtctggcag gctctcggga cggcccgtca tttggtcgaa tggcgtcgca
     2281 ccagccggtt ttgcggggtc tgcggaacgc ccctcgagat gaacccggat gagcaagtct
     2341 ttgcctgtcg ggcctgtggt taccaggcct ggcccagagt cactccggct gtgatcgtcg
     2401 cggtcaggga tggcgaccgc ctgctcctcg ggcataatgc cggttttccg gatggtctct
     2461 atgcaccctt tgccggtttt gtggagcccg gtgaaagtct tgaggaggcc tgctcccggg
     2521 aggtccggga ggaaagcgct gtcgagatcg gcgaacttcg gtatttttcc agccagcact
     2581 ggccattccc gggttccttg atgatcgctt ttacagccga gtatcgcggc ggggaagcgc
     2641 gagccgacgg ggtggaactg acggatgtcc gctggtttac ccgggacgac ccgccgcccg
     2701 ccataccccg gaatggcggg atggggcggg cattgtacga ctggttctcc ctgggaggaa
     2761 gtgtgccgaa tggatgaaaa aggaatcgga agggatggcg ggaggtggcg tgcctctccc
     2821 cttgtccggc ttgccgctgg cggactcctg atcggactgt ccgcggtctt tgtccgcttg
     2881 gtgcgggacg atgacccggg aaccgggccg gtggccgtgg ccttctggcg gatgctcttc
     2941 ggggggctgg tcctgattcc cctcgtcctg aaaagcggac gattgagacc cgccggtctc
     3001 cgggagggtt ccctgctctt cggtgcggcg cttctgtttg cctgcgacct gacactctgg
     3061 catgccagca tccacagagt tggacctggt ttggcaacca tcttggggaa tctgcaggcg
     3121 g
//
LOCUS       DAOSHE010000240         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_29465_length_3114_cov_5.217391, whole genome shotgun sequence.
ACCESSION   DAOSHE010000240 DAOSHE010000000
VERSION     DAOSHE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29465_length_3114_cov_5.217391"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            525..2444
                     /locus_tag="PLD82_07065"
     CDS             525..2444
                     /locus_tag="PLD82_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65180.1"
                     /translation="MKFRGTIRVKFAVLALLVILASSVLTSAIGYNISRSAVSRKIEE
                     FDLVNLVTARALEIETRLGRGIESSFIFAHDAALTGWFRSAEQNATLGEMALANMRTL
                     ARKGNYANVFAASATTHAFWVDGKNPPADILSQNDPDDTWFFRTMREKRPFNLNIDYN
                     RQLDNTFIFVNVLMGDPGNPDGVAGVGIELSAFAREFSIGDRHGGKSWLIATDGRVQI
                     ARDLELIGRQAGVVFGTNAAARFSALMSGAIVVRAPAGQGMLREDSYLACARVPSAGW
                     LVVYQIPESSLSGVLETMRISSLVGAAISGLLVGLLFLYGFGMIMNPLKQLARAMRSF
                     GGGDLGCRVSITSDDELGQIGTRFNEMAETLSKYNTELESIIRQRTAQLVRAEKMASL
                     GELVAGVAHEINTPVGIGVTAASDAQRRLAVLKQGFEDGTLTRSDMADYMDAAGSALE
                     MTRSNLERAAELVQSFKRVAVDRAAADMREIILDEYIDDVLRSLKPRFKKTSYQVETD
                     CPPGILMRTLPGAISQIITNLVINSLVHGFADRPEGKIRIQVAREDPMVRIVYGDDGI
                     GIPHANIGKIFEPFFTTKRGAGGSGLGLNIVWNLVTHSLGGSIECTAGVAGGVVFTLR
                     LPIALRERNGTQDEQ"
     gene            2422..>3114
                     /locus_tag="PLD82_07070"
     CDS             2422..>3114
                     /locus_tag="PLD82_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOG65181.1"
                     /translation="MVHRMSNDLMVFADDRRKDEILRRPLDAWHILVVDDEDDVHRVT
                     RLVLKDVSFLGKPIKIHSAHSRSEAEAFLASHPDISIVLLDVVMEEEDAGLKLVRYIR
                     ETLGNTVIRIILRTGQPGQAPETEVVVQYDINDYKCKTELTSEKLFTTVISALRSWRD
                     LITIDRSKKGLERIIQASAGIFEAQSLREFVSGVLMQLVSMLGLENDALYCHTSGFSA
                     TNENGELKVLAAT"
BASE COUNT          678 a          952 c          863 g          621 t
ORIGIN      
        1 aacggtctca actggtcctg gcgcctggac cggttgcctt cgcatgatga atcgctgacg
       61 cgctggttgc gccaatgcgt cgtcgagggg aagcgggcct gaggttcgat agcactccgg
      121 gagtagcatg aaaatattgc gttcgtgtca taacaatccc ggattcgcat gacgttttca
      181 cgaagatgtg ttgcgactcc gggagtccca taccaatttt atgtttcgga gacgcggtca
      241 cccgtgtttc tacgccaaag actgtgtaaa aaatgacaca ctctgtaatc cggaacactt
      301 ttttctcaaa tttgagaatt tctgccaaaa tggcctgcat ccaaaaacgc gcccaatcgc
      361 gtctctccaa aagaggcaca cccactacgc tccgcaaggg cgccctctga cgggccttga
      421 cactgttcat cataaaaaat atactgggat atctgtcgcg ccgcgatccg tgcagatccc
      481 tgttggggag atgtcccgtt tcgctgccgt caacggaggc agggatgaaa ttccgcggga
      541 caatccgggt caaattcgcc gttctggcac tccttgtcat cttggcaagt tctgtcttga
      601 ccagcgccat cggatacaac atctcccgaa gcgccgtcag ccgtaaaatc gaagaatttg
      661 atctcgtcaa cctggtcaca gctcgcgccc ttgaaatcga aacccgcctc ggacgcggaa
      721 tagaatcttc ctttatattt gcccatgacg ccgccctgac aggctggttc aggtcggccg
      781 aacagaatgc caccctgggg gagatggctc tggccaatat gcgcacattg gcccgcaagg
      841 gaaattacgc aaatgtcttt gcggccagcg ccacaacgca tgccttttgg gtggacggca
      901 aaaaccctcc ggccgacatc ctctcgcaaa acgatccgga tgatacctgg tttttcagga
      961 ccatgcgtga aaaacgtccc ttcaacctga acatcgacta caaccgccag ctcgacaaca
     1021 ccttcatctt tgtcaacgtc ctgatgggcg atccgggcaa tccggacggc gtggccggtg
     1081 tcgggattga actctccgcc ttcgcccggg agttttccat cggagacaga cacggcggca
     1141 agagctggct gatcgccacg gacggacgtg tccagatcgc ccgggacctg gaactgatcg
     1201 gtcggcaggc cggtgtcgtt ttcggcacca atgccgccgc gcggttctcc gcactcatga
     1261 gtggcgcgat cgttgtccgc gcaccggccg ggcagggaat gctgcgcgag gactcctacc
     1321 tggcctgcgc ccgcgttccc tctgccggct ggctggttgt ctaccagatt cccgagagca
     1381 gtctctccgg agtcctcgaa accatgcgca tcagctcact ggtcggggca gccatttccg
     1441 gcctcctcgt cggcctgctc ttcctctacg gcttcggcat gatcatgaat ccgctcaagc
     1501 aactcgcccg cgccatgcgc tcctttggcg gtggcgatct cggctgccgg gtctcgatta
     1561 ccagcgatga cgagctgggt cagatcggga cacgcttcaa cgaaatggcg gagacgctct
     1621 cgaaatacaa cacggaactc gaatcgatca tccgccagcg cacagcccag ctggtcaggg
     1681 ccgagaagat ggcatcactc ggagaacttg tcgccggagt tgcccacgag atcaacactc
     1741 cggtcggcat cggcgtcacc gccgcaagtg atgcccagcg ccgcctcgcg gtactcaagc
     1801 agggattcga agatggcaca ctcacccgaa gtgacatggc cgactacatg gacgccgctg
     1861 gaagcgcgct cgaaatgacc cgcagcaacc tcgagcgggc agccgaactg gtgcagagtt
     1921 tcaagcgggt tgctgtcgac cgggcggcag ccgatatgcg cgagatcatc ctcgacgaat
     1981 acattgacga tgtcctccgc agtctcaagc cacgcttcaa aaaaacctcc tatcaggtcg
     2041 aaacggactg cccgccaggt atcctgatgc gaaccctgcc gggtgcgatc tcccagatca
     2101 tcaccaatct cgtcatcaac tccctggtcc acggctttgc cgaccgtccc gagggaaaga
     2161 tccgcatcca ggtggcgagg gaagacccga tggtccgcat cgtttacggt gatgacggaa
     2221 tcggcatccc gcacgccaac atcggaaaga tttttgaacc gtttttcacc acaaaacgcg
     2281 gcgcgggcgg aagcggactg ggcctgaata tcgtctggaa tcttgtcaca cacagcttgg
     2341 gcggcagcat cgaatgcaca gccggcgtgg cggggggtgt ggtttttacc ctccgtctgc
     2401 cgatagctct acgagagagg aatggtacac aggatgagca atgacctgat ggtatttgcc
     2461 gatgaccgga ggaaggacga gatcctgcgt cgccccctgg atgcctggca tatcctggtt
     2521 gtggatgacg aagacgacgt ccatcgggta acccgtcttg tcctcaagga tgtcagtttc
     2581 ctgggaaagc cgatcaagat ccacagcgcc cactcccgga gcgaggccga ggccttcctg
     2641 gcaagccatc ccgacatctc catcgtcctg ctcgatgtcg tcatggagga ggaggatgcc
     2701 ggactcaagc tggtccgcta catccgtgaa accctgggga acaccgtcat ccgcatcatc
     2761 ctgcgcaccg ggcaaccggg ccaggctccc gaaaccgaag tggttgtcca gtacgacatc
     2821 aacgactaca aatgcaaaac cgagctgact tccgaaaaac tttttacgac cgtcatttcc
     2881 gccctgcggt cctggcgtga tctgatcacg attgaccgga gcaaaaaagg gctggagcgg
     2941 atcatccagg cctcggccgg aatcttcgag gcacaatccc tgcgcgagtt cgtgagcggc
     3001 gtcctgatgc aacttgtgtc gatgctcgga ctggagaacg acgcgctcta ctgccatacc
     3061 tccggattct ccgcgacaaa cgaaaacggg gaactcaagg tcctggccgc aacc
//
LOCUS       DAOSHE010000241         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_30335_length_3051_cov_4.345794, whole genome shotgun sequence.
ACCESSION   DAOSHE010000241 DAOSHE010000000
VERSION     DAOSHE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30335_length_3051_cov_4.345794"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLD82_07075"
     CDS             complement(<1..582)
                     /locus_tag="PLD82_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOG65182.1"
                     /translation="MKERSGPTIVIFGASGDLARRKLVPALWHLAGRAALPPGLRVIG
                     TGRTGFVDQAYQEHIRDAVRATVAGVDPDALDAFSRAFSFVSIPEGGSFAGLKAALTA
                     DGGCGDCIFYLAVPPALYAGIAAGLAETGMMDERDGYRRLIVEKPFGYDLESARALQD
                     ALLGCCREDQIYRIDHYLGKETVQKILAFRFSNA"
     gene            complement(677..1432)
                     /gene="truA"
                     /locus_tag="PLD82_07080"
     CDS             complement(677..1432)
                     /gene="truA"
                     /locus_tag="PLD82_07080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG65183.1"
                     /translation="MRNLEMVIQYDGGRYEGWQRLGGGKAGRTIQGVLEEALSGITGA
                     PVEVIGSGRTDAGVHALGQVANFHTRSGLSCGEIVSALERTLPPDLAVRTVKEADPRF
                     HARFHARAKRYAYRLDTGAVHDVFMRRYALHIPRPLDWDEMRRAADCLCGRHDFTSCT
                     NARLKEKSAERVIHSIEFVRDGQFVDLEFFGDGFLHNMVRILAGTLVEVSSGRLAASD
                     IPGILEARSRQAAGPMLPAHALVLVEVLYGQES"
     gene            1678..2634
                     /gene="arcC"
                     /locus_tag="PLD82_07085"
     CDS             1678..2634
                     /gene="arcC"
                     /locus_tag="PLD82_07085"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG65184.1"
                     /translation="MSKRVVVLALGGNGIQPSNSKGSYEDQFKNIDITAQQIAHLIES
                     RYKVVITHGNGPQVGTIMTQMAAAADSVPEQPMRICGAMTQGQIGYLLQSRLQYHLLK
                     LRGAPVPVCTVVTQVVVDKDDPAFQNPTKPVGSFYSKEEADAIAKANGYTMKEVAPEK
                     PKPWRRVVCSPKPQDIVEIDSITQLLDNHVVVISCGGGGIPVVRKTDGECEGIDAVID
                     KDYTAALLAKKVDADYLMILTDVEKVALNYGKPDQKWLDTLSSDEAEKMHKSGEFPGG
                     SMGPKVQAAIDFVRWGGEGAIITSLEKAREALSGKTGTHFKK"
     gene            2819..>3051
                     /locus_tag="PLD82_07090"
     CDS             2819..>3051
                     /locus_tag="PLD82_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65185.1"
                     /translation="MRGYILTTIFSALLLAAGATTCGRGDGMSILFLHPPGVETTPVQ
                     QGLTFAVEELNSRDGLGGRTLVLETLAVSLHTN"
BASE COUNT          643 a          979 c          867 g          562 t
ORIGIN      
        1 ggcattcgaa aaccggaatg cgaggatttt ctgtactgtt tccttcccga ggtagtgatc
       61 gatgcggtag atctggtcct cgcggcagca tccgaggagg gcatcctgga gcgcacgggc
      121 cgattcgaga tcatacccga aaggtttttc gacgatcagt ctcctgtacc cgtcacgctc
      181 gtccatcatg cccgtctcgg ccagtccggc agcgattccc gcatagagtg ccggcgggac
      241 cgccaggtaa aaaatgcagt ctccacaccc cccgtcagcc gtcagggctg ccttgaggcc
      301 ggcaaaggat cctccttcag gaatggagac gaaggaaaaa gcgcgcgaga atgcatcaag
      361 tgcatccgga tccaccccgg ccaccgtcgc acggacggcg tcccggatgt gttcctggta
      421 cgcctgatcc acaaacccgg tccgcccggt cccgatcacc ctcaggcccg gcggcagcgc
      481 ggcccggcct gcgagatgcc agagtgcggg aaccagtttg cgacgggcga gatctcccga
      541 tgccccgaat atgacgatcg tcggcccgga ccgttctttc atggagccct cctctgcggg
      601 tcataccaga ttgcatgccc ataccggtaa tatatgccat tttttatatt atggatacca
      661 tggaaagagt cgggcatcat gactcttgtc cgtagagaac ttccaccagg accagtgcat
      721 gagccgggag catcgggccg gccgcctgcc ttgagcgcgc ctcaaggatg cccgggatgt
      781 ccgaagcggc gagtctgccg gaactcacct cgacgagagt cccggcaagg atccggacca
      841 tgttgtgcag gaacccgtcg ccaaaaaact ccagatcgac gaactgcccg tcacggacaa
      901 actcgatgga atggatcacc cgctcggcgg acttctcctt gagccgggca ttggtgcaac
      961 tggtgaaatc gtgccgcccg cacagacaat cagcagcccg gcgcatctcg tcccaatcca
     1021 acggacgggg gatgtggagc gcatagcgtc tcatgaagac atcatgaacc gccccggtat
     1081 ccaggcgata ggcgtagcgt ttggcacggg catggaaccg ggcgtggaac ctgggatcgg
     1141 cctccttgac cgtccggacc gccaaatcgg gagggagggt ccgttcgagc gcggacacaa
     1201 tctccccgca ggagagaccg gaccgggtat ggaaattggc gacctggccc agggcatgca
     1261 ccccggcatc cgtccggccg gacccgatca cctcgaccgg ggcaccggtg atccctgaga
     1321 gggcctcctc cagcacaccc tggatggtcc tcccggcctt cccgcccccg agcctctgcc
     1381 agccttcgta gcggcctccg tcatactgga tcaccatttc aagattgcgc atagcccccc
     1441 ctggaatccc ttgagtatac agcgttttgg cctcatgcga catccgcgaa acgctctgcc
     1501 ctcccgtgag agaatcccgc gcgggccgga agatccgggg aaaggattcg ggtgtcagag
     1561 cggtcccgga ctccacaaac cggtaatgtt tatttctctt gctgacaaac aggcagaaca
     1621 gctatactgt gcctgctgtt cacaaaaaga ccagttcacg tatgaggtaa ccagacaatg
     1681 agtaaacgag tcgtcgttct tgccctcggt ggcaacggaa ttcagccctc caattcgaag
     1741 ggttcctacg aagatcagtt caaaaacatc gatatcacag cccagcagat cgcgcatctc
     1801 atcgagagcc gctacaaggt tgtcatcacc cacggaaacg gcccccaggt cggaacgatc
     1861 atgacacaga tggccgctgc cgccgactcg gttcccgaac aacccatgcg gatctgcggt
     1921 gcgatgaccc agggccagat cggctatctt ctccagagcc ggctccagta ccaccttctc
     1981 aaactccgtg gtgcgcccgt tccggtctgc accgtagtca cccaggtcgt cgtcgacaag
     2041 gacgatccgg ccttccagaa tccgaccaaa ccggtcggct cgttttactc caaagaggaa
     2101 gcggacgcca tcgccaaggc caatggctac acgatgaagg aagtcgctcc cgaaaagccg
     2161 aaaccctggc gccgggttgt ctgttcgccg aaaccgcagg atatcgtcga aatcgactcg
     2221 atcacccagc tcctggacaa tcacgtcgtt gtcatctcct gcggcggtgg cggcattccg
     2281 gtcgtccgca agaccgacgg agagtgtgag ggcatcgatg ccgtcatcga caaggactac
     2341 accgccgccc tcctcgccaa gaaagtggat gcggactatc tgatgatcct gaccgatgtc
     2401 gagaaggtcg ccctcaatta cggcaagccg gaccagaagt ggctggacac cctttccagt
     2461 gacgaggctg aaaaaatgca caagtccggc gagttccccg gtggctcgat gggccccaag
     2521 gtccaggcag cgatcgactt cgtccgctgg ggtggcgaag gtgcgatcat cacctcgctg
     2581 gagaaggccc gcgaagccct ctccggaaaa accggaaccc atttcaaaaa atgagatcct
     2641 cccgctgttc caaagagctg tcccgaaggg ggcagctttt tttcggatcg gcctgcccct
     2701 gattcctgtt ctgccagtgg caccatgccg ttcccgcggg aggcccggtg ccacgccgcg
     2761 agagcggcag tcgcgtttcc cccttgttgt ccgcgggctg ccatggcata ctgcccccat
     2821 gcgcggatac atcctgacca cgattttctc agccctgctc cttgctgccg gagcgacgac
     2881 ctgcggccgg ggcgacggta tgtccatcct gttcctgcat ccgccgggtg tcgaaacgac
     2941 accagtgcaa cagggcttga cctttgccgt cgaggagttg aattcccggg atggactcgg
     3001 aggaagaacc ctggtgcttg aaacacttgc cgtctccctg cacaccaatg a
//
LOCUS       DAOSHE010000242         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_30385_length_3047_cov_3.514706, whole genome shotgun sequence.
ACCESSION   DAOSHE010000242 DAOSHE010000000
VERSION     DAOSHE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30385_length_3047_cov_3.514706"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..373)
                     /locus_tag="PLD82_07095"
     CDS             complement(149..373)
                     /locus_tag="PLD82_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOG65186.1"
                     /translation="MEVIGEQGSLESSGKTDTCILVLLGSHPDMTLEEVAAELGITVR
                     AVEKQVSKLRQVGRLRRIGPAKGGHWEVVE"
     gene            complement(762..2171)
                     /locus_tag="PLD82_07100"
     CDS             complement(762..2171)
                     /locus_tag="PLD82_07100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG65187.1"
                     /translation="MHIDDRYSDPVLIGSGGMAEVYRAHDRRLDRDVAIKIIRQETSD
                     KPQFRQRFKREIRMLTSLQHPNIIPVYDCSAEESSTYYVMALLEGKTLAEYVPGGHIS
                     GLFYKTVMEGMLAALSCAHGKGVIHRDIKPENIFISTNGDPVLMDFGIAGAVDEEATC
                     LTQAGSFIGTCLYASPEQLNGERLTPATDIYSLGLVLQAMLCGGLPYSGTMSQIIQQK
                     TMQPTPEPPSTHPVYEMLPALKSVLPRMLAREPEQRYGSCEEILRDLRRATSSVSSGS
                     AAESQPGVKPRVREEASGGPVTKRITSGFKWVAVATVVAAILILGLKGKSRIAITEFD
                     ASESISGVVVDGVSYSFHSGNLFDDNPATSWQPKDIVGGWFTVRLPGKNSYEIRIING
                     FASRQGLYDWYYRNNRVRSVDVISSEGVKAITLQDGNQEYQSLGTYETDFFQIRITGI
                     YPGQKWNDTAISDAIVFRR"
     gene            complement(2359..2832)
                     /locus_tag="PLD82_07105"
     CDS             complement(2359..2832)
                     /locus_tag="PLD82_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG65188.1"
                     /translation="MKAGMILCVMAMVCTMGCGNDTGMITANLVNLRSGPSTDSMVVA
                     RLYANQKVRIIEKQVMTTKQEGQGVIGKSGILNKQAYIYIGRQPYLMNQAKAVRIIGL
                     RGSAYMVEVEVDTMQKAIGYVKMVDVEILNSNVWYRVRTENGQEGWVFERFIVRT"
BASE COUNT          692 a          917 c          706 g          732 t
ORIGIN      
        1 ccaggacaaa acttctccct tcgcgttgct ccgggacttc gcatgtttta gccccaaggg
       61 gaaggagtat gaatctcttg ttattgagtg tctgaccagg ttgtacaggt tcacccttct
      121 ctgccgcgag tgatccggga aacactgcct actcgaccac ttcccaatgc ccgcccttgg
      181 ccggaccgat tcggcgaagg cgtcccactt gccgtaattt ggagacctgt ttttcaactg
      241 ctctgactgt aatacccagt tccgcagcta cttcttccag cgtcatgtca ggatgactac
      301 ccagcagcac aaggatacaa gtgtctgttt tccccgaact ttccagtgaa ccttgttcac
      361 caatcacttc catcccttgt ttccttcgta tgcaggccgt gaagagacag ccatcccgat
      421 cgtcctcaaa ctcaatgtcc gtccagagct ccaacgcccg cttgatcccg gagccaagac
      481 catgatatgg cagcaggccc ttggccacat aggaaacgag gatcgggttg tgtgtattcg
      541 agttgccgac cctgatttac aaagtgaatt tagtcatgca aggtctgaat gcccgttttc
      601 tgaacaatac ccagaaaatt gttttagcct tgccaatccg cccgcccatc aacattgcaa
      661 atcaaagcca tagacaaagg gcactcgaaa gcaattcatt gccagttcag cccacagaca
      721 aaaccgttct tgctatgagc attgagtatg tcaaacctgc actacctcct gaatactatc
      781 gcatcggaga tagccgtatc gttccacttc tggccaggat aaattcccgt aatacgaatc
      841 tgaaaaaaat ccgtctcata cgttccaaga ctttggtact cctgattacc atcctgtaac
      901 gttatggctt tcactccttc actcgaaatg acgtcgacgc tgcgtacacg attgttgcga
      961 taataccagt cgtacaaccc ttgtctggat gcgaatccgt tgataatccg tatttcgtat
     1021 gaattcttgc ccggcaaacg aactgtaaac cagcctccga cgatatcttt cggctgccaa
     1081 gatgttgccg gattatcatc gaacaagttt ccggaatgga aactgtagga aaccccgtcg
     1141 accacaaccc cggatatcga ctcgctggca tcaaattcgg taatggcaat ccggctcttt
     1201 cctttcaatc ccagaatcag aatcgcggcc accactgtag ctacggcgac ccatttgaaa
     1261 ccactcgtaa tccgcttggt aaccggcccg ccggatgctt cttcccgcac ccggggcttc
     1321 acgccgggct ggctctccgc cgccgaaccg gatgataccg atgaagttgc cctgcggaga
     1381 tcccgcagaa tttcctcgca acttccgtat ctctgctccg gttccctggc cagcatccgc
     1441 ggcaagacgc ttttcagggc aggaagcatc tcgtaaaccg gatgtgtcga cggcggttcc
     1501 ggcgtcggct gcattgtctt ctgttgaatg atctgggaca tcgtcccgga gtagggcagt
     1561 ccgccgcaga gcatggcctg gagtaccagt cccaaagaat agatgtctgt cgctggagtc
     1621 agcctttccc cgttgagctg ttcgggagac gcatacaaac aagtgccgat gaaactcccg
     1681 gcctgcgtca gacaagtcgc ttcctcgtca acggcgcccg ctatcccgaa atccatcagg
     1741 acaggatcac cattggtgct gataaaaatg ttttctggct ttatatcccg atgaatgaca
     1801 cctttgccat gggcacagga caaggcagcc agcattcctt ccatgacagt cttgtaaaac
     1861 aggccagaaa tatgtccacc ggggacatat tcagccaacg tctttccttc gagcagtgcc
     1921 atgacatagt acgtcgagga ttcttcggca gagcagtcat ataccggtat gatattggga
     1981 tgctgtagac tggtcagcat ccggatttcg cgcttgaacc gctggcgaaa ctgcggcttg
     2041 tcacttgtct cctgacggat gatcttgatc gccacgtcac gatccagacg ccggtcatga
     2101 gcgcgataca cttcggccat gcctcccgaa ccgatcagga ccggatcgga atatcgatca
     2161 tcaatatgca tcacaagtcg cccccaaaac tatttttctt gtctgttgaa ccggttgagc
     2221 cagcctgttc aaaaccgctg cccacctgga cagtcactgg tcagccattc caggcagtgc
     2281 gaaggacact cccccaaaaa cagcaatata ccatccgcct taaccgcgga gacgcaccac
     2341 cccgttcggg atgaccggtc aggtccggac gatgaatcgt tcaaaaaccc agccctcttg
     2401 cccgttttcg gttcggactc tgtaccagac attcgagttg aggatctcga catccaccat
     2461 tttgacataa ccgatggctt tctgcatagt atcgacttcg acttccacca tataggcact
     2521 gcctctcaag ccaatgatcc ggacagcctt ggcttggttc atcagatacg gctgtcgccc
     2581 gatatagata taggcctgct tgttgaggat gcccgacttc ccgataacgc cctggccctc
     2641 ctgtttggta gtcataacct gtttctcgat gatccggacc ttttggttgg cgtacagccg
     2701 ggcgacgacc atgctgtccg tcgagggtcc cgaccgaaga ttgaccagat tcgcggtaat
     2761 catccccgta tcgttgccgc agcccatggt gcacaccatg gccatgacac ataggatcat
     2821 cccagccttc attctcctgt cctccgcttc gttttcttac cggccccggc ggtcaccgga
     2881 ccgtcagtcc ccggctcctt cgccgtcatt ccggtttgcc gacgccgacg gggcattgta
     2941 ccgctgcaac agccggacaa ttctgtactg cccattccga cgggcaatat cgatataata
     3001 cgattcgcga ttctctccgg gcatccggat ccggacggaa agatccg
//
LOCUS       DAOSHE010000243         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_30616_length_3031_cov_3.317204, whole genome shotgun sequence.
ACCESSION   DAOSHE010000243 DAOSHE010000000
VERSION     DAOSHE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30616_length_3031_cov_3.317204"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLD82_07110"
     CDS             <1..469
                     /locus_tag="PLD82_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65189.1"
                     /translation="WFFSDNFSVSLLLAFYETSIESYRLDTGNYESDMRTEASMATIG
                     IDFWFNPQRDLGLLTGFKPFGEFDICIGLSFGYFSASPDGTGFFMRLQNDPSAIMDNS
                     FFLTMLHTGFSFNLISTFFMRLDWYTGRLENILSDGIGGDFVNAVSISFVFRY"
     gene            complement(536..982)
                     /locus_tag="PLD82_07115"
     CDS             complement(536..982)
                     /locus_tag="PLD82_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG65190.1"
                     /translation="MSAETILDFAISQEEEASAFYLELASRVKNPGMKTVFEDFAREE
                     QGHKARLETIKSGGTLHAAGQKVLDLKISEYLVEIDPDKDPLDYQNALIIAMKKEKAA
                     YRMYLDMADGVSDAGLRELFLSLAQEEAKHKLRFEVEYDERILSEN"
     gene            complement(1032..2999)
                     /locus_tag="PLD82_07120"
     CDS             complement(1032..2999)
                     /locus_tag="PLD82_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65191.1"
                     /translation="MHERIPVALLFAMNQDLVPGCDLATDVCYLPLLEVFEAHPGIRF
                     NLEISGTLFDWAAWHRPRLLDTIRRMHGSGQLELVASTFSRNILYCSQAATVADSIRF
                     HMDLLAKNLGAHPRGFLNPGKVWSHEYIPQIAGAGLEWTLVDERVLRGSGIHKKVNCP
                     RRGVSDGQEITILTDSLAGTAGFHDAVAHFSLSRYEALVQYLAELRNESPDGLFTYCE
                     HAERSGLWQYLEQDGDPKTIIKHWDRMLTQLERDERLETVCITTWLHRTKVHERLETS
                     VDGEPEWIAEVFAIPGTRWNEGGFRDWFDFAEHSSEMRYFREFYAELAGRIANAASAL
                     ATTRLPAELRMACERLIDDARFGLVLHQYELGFSEQDVRGFSRRELARVISVRLALVD
                     AILADRTGFSISDVNDDGLPEILWLDAGNFYVFSKMGGRLLYWFDLLSAREMIGCEHV
                     SHYEELFRDDNHVVPEVGIGDGLWTNLEQRPQESVETGRYLLRRRGLLDTVVHRVSGE
                     SDGTVVNLAHHEMPFALKQERIEFQYEAEGLALLKILAIREDGLDVTWHVALPGDDSA
                     EVAIVSETAFSPQHEDVLREGLPRDWYQCSGRSVTTPMFEVGLIADGAKNVSSVEQAF
                     AVGFIAEYSGQTEDVFTAECRLFKKRLTAGA"
BASE COUNT          702 a          906 c          726 g          697 t
ORIGIN      
        1 ctggtttttt tcggacaatt tctcggtctc gctcctcctt gccttctacg aaacatccat
       61 cgaaagctac cgtctggata ccggaaatta cgagtcggat atgcgcaccg aagcgagcat
      121 ggcaacgatc ggcatcgatt tctggttcaa cccccagcgt gatcttggtt tgctcaccgg
      181 attcaagccc tttggcgagt tcgatatatg catcgggctc tcgtttggct acttttcggc
      241 atcacctgac ggaacggggt tcttcatgag actccagaat gacccatccg ccatcatgga
      301 caactcgttt ttcctcacca tgctccatac cgggttttcg ttcaacctca tcagcacatt
      361 tttcatgcgt cttgactggt acaccggacg tctagagaat atcctgtcag acggaatcgg
      421 cggtgatttt gtgaatgccg tttcgatctc gttcgttttc cggtactgag cgggaaaaag
      481 aaaccgccct cggtcgggcg gttgtcacca tcaatggctc tgtcaacagg tgtaatcaat
      541 tctcgctgag aatccgttcg tcatactcca cttcaaagcg gagtttgtgc ttggcttctt
      601 cctgcgccag cgagagaaag agttcccgga gtccagcatc ggaaacacca tcggccatgt
      661 cgagatacat ccggtaggcc gccttttcct ttttcatggc aatgatcagg gcattctggt
      721 aatccagggg atccttgtcc ggatcgatct cgaccaggta ttcactgatc ttgagatcca
      781 gcaccttttg cccggcggca tgaagcgtgc cgcccgactt gatcgtctcg agcctggcct
      841 tgtgcccctg ttcttccctg gcgaaatcct caaacacggt cttcatgccc ggattcttga
      901 cccgtgaggc caactcaaga taaaacgcac tggcctcttc ttcctgtgat attgcaaaat
      961 caagaatggt ttccgcactc atctgtttct cctttaatac agaatatact gggtgaaata
     1021 agccttcgca atcaggcgcc tgcggtcagc cgcttcttga agagacggca ttccgcagta
     1081 aacacatcct ctgtctgtcc ggagtattca gcgatgaacc cgactgcaaa agcctgctcg
     1141 acactcgata catttttggc gccatcggca atcaaaccaa cctcgaacat gggcgttgtg
     1201 acactccgcc cggaacactg ataccagtcc cggggaagcc cctcccgaag gacatcctca
     1261 tgctgtggag aaaaagcggt ttccgatacg attgccactt ctgcactgtc atccccgggc
     1321 agggccacgt gccaggtgac atccagaccg tcctcgcgga ttgcgagtat cttgaggagg
     1381 gccagacctt ccgcttcgta ctggaactcg atcctctcct gcttgagagc aaagggcatc
     1441 tcgtgatgcg caaggttgac aacagtcccg tcactctcac ccgaaacacg atggacaacc
     1501 gtgtccaaga gccctcttct gcgcaacagg tatctccctg tttcaacgct ctcctgtggc
     1561 cgttgctcaa gatttgtcca gagaccgtcg ccgattccca cctcggggac cacatgattg
     1621 tcatccctga aaagttcctc atagtgggaa acatgttcgc agccaatcat ttcccgcgca
     1681 gaaagaagat cgaaccagta gagcagccgg ccgcccattt tcgaaaaaac ataaaaattc
     1741 cccgcatcaa gccagagaat ctccgggagg ccatcgtcat tcacatcgct gattgaaaac
     1801 ccggtcctgt cagcaaggat agcatccacc aaagcgagac gcacagaaat caccctggcc
     1861 agctcacgtc tgctgaaccc cctgacatcc tgttccgaaa atcccagttc atactggtga
     1921 agcacgagac cgaaccgggc atcatcgatc agccgttcac aggccatccg cagttcggcg
     1981 ggaagccgtg tagtcgcaag cgcggaagcg gcattcgcaa tcctgccggc aagctcggca
     2041 taaaattccc ggaaataccg catctccgaa gaatgttcgg caaaatcaaa ccagtcccgg
     2101 aagcctccct cgttccagcg tgtcccgggg atagcaaaca cttcggcaat ccactccggt
     2161 tcaccgtcaa cactggtctc cagccgttca tgaacttttg ttctgtgaag ccaggtggtt
     2221 atacacacag tctcgagcct ttcgtcccgc tcaagttgcg tcagcatcct gtcccagtgt
     2281 ttgatgatgg tcttcgggtc accatcctgc tccaggtact gccagagacc ggaacgttcc
     2341 gcatgctcgc aatacgtgaa gagaccatca ggactttcgt tccgcaattc agccaggtac
     2401 tgcacgaggg cttcataacg gctgagagaa aaatgggcaa cagcatcatg gaaccctgca
     2461 gtacccgcaa gactgtctgt cagaatcgtg atctcctgcc cgtcggacac accccgccgg
     2521 gggcagttga ctttcttgtg gatcccgctc cctcgaagaa cacgctcatc caccaaagtc
     2581 cattcaagac ctgcgccggc tatctgcgga atgtactcat gcgaccagac ttttccgggg
     2641 ttgagaaatc cgcggggatg cgccccgaga tttttggcca gcaaatccat gtgaaaacgg
     2701 atggaatcgg ccaccgtcgc agcctgactg cagtagagaa tattcctcga aaaagtactt
     2761 gccacaagct cgagttgccc ggagccatgc atgcgccgga tggtatccaa gagccttggt
     2821 ctgtgccagg ctgcccagtc aaacagggtc cccgagatct cgaggttgaa cctgattccg
     2881 ggatgtgcct caaatacttc aagcaatgga agatagcaaa catctgttgc gagatcacac
     2941 cccgggacca ggtcctggtt catcgcaaac agcaaagcga cgggtattct ctcgtgcatt
     3001 gaccttcctc cacaacaatc tcttcccggc a
//
LOCUS       DAOSHE010000244         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_30748_length_3021_cov_4.479434, whole genome shotgun sequence.
ACCESSION   DAOSHE010000244 DAOSHE010000000
VERSION     DAOSHE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30748_length_3021_cov_4.479434"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLD82_07125"
     CDS             complement(<1..511)
                     /locus_tag="PLD82_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG65192.1"
                     /translation="MSERIALEVRDDGIAIMRMQDVERKNVFADDFLNEMVETFEKAE
                     KLRPKVMIFCGLPEVFSAGAEKKNLMDLCDGKVHVKDLVISEKLVHSPFPVIAAMEGH
                     AVGGGLVMAACCDMVIGARESRYGVVFMGLGFTPGMGCTKLLQGLVGNQIANEMMFTA
                     KRFRGSELAG"
     gene            complement(504..1346)
                     /locus_tag="PLD82_07130"
     CDS             complement(504..1346)
                     /locus_tag="PLD82_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG65193.1"
                     /translation="MSGAENEDVRVHYAEDAGLALVTLADSAHGNRINAARLAALHEA
                     FGKAEASESVRAIVLRSDGTRFCLGMDFSFFLGTDSGGLTAEEAVKAYSGLLTRMRVS
                     SKPVIALVDGDVKAGGIGLVAAADIVIASERSQFQLSEVLLGLIPANVLPFLVERVGQ
                     RRASWLTMTARTLGAEGAREIGLVDEVFATDALEKETRAFMRTIMRADPHALAATKSF
                     ISEIAGLERDEATQAAARSLLELAARPEVRYAVAAFEDGGLPAWSLRFKPEHNLWMGE
                     KTDE"
     gene            complement(1333..2571)
                     /locus_tag="PLD82_07135"
     CDS             complement(1333..2571)
                     /locus_tag="PLD82_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOG65194.1"
                     /translation="MNRVGIEKINIYGSSLCLDQQELALARGRDPERVLRDFHINTRS
                     LNPPYEDTVTMGANAARAMLTEEDKKSIGMIIVGTEGSVDFGKPISTDIQSALGLPNT
                     IRSYETKHACYSGVAALDTAVNWIASGLNKGKKALVIAADFSRKHLGKDHEFVLGGSA
                     AAILVSENPKILEYETMKKGSYSNDVYDTFRPTAKAEMGNNEVSLYSYQEAVEGAYNE
                     YLAQIDGAIDSAEKFDAYFNWNIYHMPFPGMALLGHRVVCKVSGLRKKSEIEESFERK
                     VLPSLHFAKRVGSTYGGSNFVGLCGLLASAPVKDGDRIGFFSYGSGFMGEFYSGIVLP
                     GAKEAILKMGIGEAMDARRQVSVAEYERIENLRESYIENPDFVPDFSVADNWYKQHYE
                     GKGYLVLKEVKDYYRRYEWS"
     gene            complement(2619..>3021)
                     /locus_tag="PLD82_07140"
     CDS             complement(2619..>3021)
                     /locus_tag="PLD82_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG65195.1"
                     /translation="GPFLLMRAFTMHFMSNRFGRIVNISSLAEGGCSGQANYAASKSG
                     LMGLTQTFAKEYGPKGITANIVTVGYVPTDMTKDNMAEQLHQFWMKHCPLKRPGKGEE
                     IADLVHFLCSERGAFINGERIRVSGGLTYAP"
BASE COUNT          618 a          931 c          832 g          640 t
ORIGIN      
        1 tcccggccag ctcgctcccg cgaaagcgct tggcggtaaa catcatctcg ttggcaatct
       61 ggtttccgac cagtccctgc aggagtttgg tgcagcccat cccgggtgta aacccgagtc
      121 ccataaatac gacgccatac cggctctcgc gggcgccgat gaccatgtcg cagcaggccg
      181 ccatcacaag gccacctccg acggcatgtc cttccatggc cgcaatcacc ggaaagggtg
      241 aatgcaccag cttttcggaa atcacgagat ccttgacgtg cactttaccg tcacagagat
      301 ccatcagatt ctttttctcg gccccggctg aaaaaacctc tggcaagcca caaaaaatca
      361 tgaccttggg ccggagtttt tcggcttttt cgaaggtttc aaccatttcg ttgagaaaat
      421 cgtcggcgaa gacattcttc cgttcgacat cctgcatccg catgatggca atcccatcat
      481 cacgcacttc aagggcgata cgctcactca tcggtctttt ctcccatcca gagattgtgt
      541 tcgggcttga agcgcaggct ccaggccggc agtccgccat cttcgaaggc ggccacggcg
      601 tagcgcacct cggggcgggc agccagctcg agcaggctcc gggccgcggc ctgggtggcc
      661 tcgtcccgct cgagtccggc aatttcgctg ataaagctct tggttgccgc cagcgcatgg
      721 gggtcagcac gcatgatggt gcgcatgaac gcccgggttt ccttttcaag ggcatccgtc
      781 gcaaagacct catccaccag gccgatctca cgtgcacctt ccgcgcccag tgtccgggcc
      841 gtcatcgtca gccaggatgc gcgacgttgg ccaacccgct cgaccaaaaa gggcaggaca
      901 ttggccggaa tcaacccgag caggacttcc gagagctgaa actgggagcg ctctgacgcg
      961 atcacgatat ccgcagcggc caccaagccg atcccgccgg ccttgacatc cccgtcgacc
     1021 agcgcgatca ccggtttgga cgaaacccgc atccgggtga gcaggcccga gtacgccttg
     1081 acggcctcct cggctgtcaa tccgcccgag tctgttccca gaaaaaaaga aaaatccatg
     1141 ccgaggcaaa aacgtgtccc gtccgaacgc agcacaatgg cgcgcacgga ctcggacgcc
     1201 tcggcttttc cgaaggcttc gtgcagggcg gccaaccggg ccgcattgat cctgttgccg
     1261 tgtgcactgt cggccaaggt caccagagcc agcccggcat cctcggcgta gtgcacgcgt
     1321 acatcttcgt tttcagctcc actcatagcg gcgataatag tcctttacct ctttgaggac
     1381 gaggtaaccc ttgccctcat agtgttgttt ataccagttg tccgcaaccg aaaaatccgg
     1441 gacaaaatcg ggattttcga tatagctttc gcgcaggttt tcgatgcgct cgtactcggc
     1501 caccgaaacc tggcgccggg catccatcgc ctcgccaatc cccatcttga ggatggcctc
     1561 tttggcgcca ggcaaaacaa tcccgctgta gaactcgccc atgaacccgg atccgtagga
     1621 gaagaaacca atccggtcgc catccttgac tggagcgctg gccaagaggc cgcagagccc
     1681 gacaaaattg ctcccgccat aggttgaacc aacccgtttg gcaaagtgca gggaggggag
     1741 caccttgcgc tcaaagctct cctcaatttc ggactttttc cgcaggccgc tcaccttgca
     1801 gaccacgcgg tgcccgagaa gagccatccc agggaagggc atgtgataga tgttccagtt
     1861 aaaatacgcg tcgaactttt cggctgaatc gatcgccccg tcaatctggg cgaggtactc
     1921 gttataggcg ccttcgacgg cttcctgata gctgtagagg ctgacctcgt tgttgcccat
     1981 ctcggccttg gccgtcgggc ggaaggtgtc atagacatcg ttggaatagg atcccttttt
     2041 catcgtctcg tattcgagga tcttgggatt ctcgctgacg aggatcgccg cggcagatcc
     2101 acccaggaca aactcgtgat ccttgccgag gtgcttgcgg gaaaagtcgg ccgcgatcac
     2161 cagggccttt ttccccttgt tgagtccgga cgcgatccag ttgacggccg tatcaagcgc
     2221 ggccaccccg ctgtagcagg cgtgcttggt ttcgtagctg cggatggtgt tgggcagacc
     2281 cagggcactc tggatatcgg tggagatggg cttgccaaaa tcgacggagc cctcggttcc
     2341 gacaatgatc atcccgatgg actttttgtc ctcttcggtc agcatcgctc tggcggcatt
     2401 ggcccccatg gtgacggtat cctcgtaggg aggattgagc gagcgcgtat tgatgtggaa
     2461 atcacgcaac acccgctcag gatcgcgccc cctggccaga gccagctcct gctggtcaag
     2521 acagagcgag ctgccgtaaa tgttgatctt ttcgatacct accctgttca ttggttcccc
     2581 cgattatggt tttgggatag tctggacaac ccgcgccctt acggggcgta ggtcaatcct
     2641 ccggagacac gaatccgttc tccattgata aaggcgcccc gctcggagca gaggaaatgc
     2701 accagatcgg caatctcctc acccttgccg ggccgcttta acgggcagtg cttcatccag
     2761 aactggtgaa gctgttctgc catattgtct ttggtcatgt cggtcggaac atagccgact
     2821 gtcacgatat tggctgtgat tcccttgggt ccgtattcct tggcaaaggt ctgcgtcaac
     2881 cccatcaggc cggacttgct ggcggcgtaa ttggcctgcc cgctgcagcc tccctcggcg
     2941 agcgaggaga tattgacgat ccgcccaaag cggttggaca taaaatgcat ggtaaaggcc
     3001 cgcatcagca aaaacggccc g
//
LOCUS       DAOSHE010000245         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_30787_length_3019_cov_3.419366, whole genome shotgun sequence.
ACCESSION   DAOSHE010000245 DAOSHE010000000
VERSION     DAOSHE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30787_length_3019_cov_3.419366"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1112)
                     /locus_tag="PLD82_07145"
     CDS             complement(174..1112)
                     /locus_tag="PLD82_07145"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG65196.1"
                     /translation="MSDFDKNALLAAVEHIPALPQLVSSINAIDETSDSAGYELQKLL
                     ASNEDLDAKLLKVVNTIYYAYPDPIASISEAAALLGYTLTRNLLIATAFIDSFRQPGK
                     VKFDRARFWKHSLATARTAKEFAEAEGGFSEEETRDLYLAALFHDFGIIILESFFPEM
                     FQRVLDGVASGKSYVEAEAASLGEWGHPAIGRVVATQWGMPSSLIEAVACHHAPAACS
                     DPNLKRFVSLIHLAHYGTELAGFGIFAGGAPAPFDATIYSTLSLTKETVKQLTIRQLD
                     AKDEINEIATILIAQSGAAAMAKKAARPKQAPGKKK"
     gene            1257..>3019
                     /gene="lon"
                     /locus_tag="PLD82_07150"
     CDS             1257..>3019
                     /gene="lon"
                     /locus_tag="PLD82_07150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG65197.1"
                     /translation="MGNPVRGEDASLVVVDQVLPIKLPLVPLFSRPLFPEMLSPMMIT
                     DKHSIETIDYALAHGGYVGFILVRDPDKEPGDKGNLHDVGSVARILKRVHLPDGGINI
                     FVNAVKRFRLRKLLADEGSMLLAAVTYEHEEESEDDETKALTRAIFSLVREITENNPF
                     FTEEVRMTLVNVKNPARLADFIASILNISREEQQTVLESCVLKERLSLVLKLLTREKD
                     LLELQHKIQDQINEKISKQQREFFLKEQLKAIRQELGMEVDEKTGDIARFRERLTALA
                     LEGEAGEKLDAEINKLSATDQHSPEYTVIRNYLETALVLPWKELTDDRIDLEKAGKLL
                     SAEHFGLDEVKKRILEFLAVQVLKKDAAGSILCLVGPPGVGKTSLGKAIAASMNRKFF
                     RFSLGGMRDEAEIKGHRRTYIGAMPGKIIEALKITKTANPVLMLDEIDKLGQSYQGDP
                     SSALLEVLDPEQNASFRDHYLDLPFDLSRVFFITTANTLDTIPGPLLDRMEVIRLSGY
                     ISEDKAQIASRYLIPRALEANGLPKGHVSIGKSVLVEIANGWAREAGVRNLEKRINRI
                     MRKLAWEYVSEGKIDPVTITSA"
BASE COUNT          654 a          799 c          891 g          675 t
ORIGIN      
        1 ttcgtgcgga tggttttttc ggggacgatg cttgttccgg catggtgttc tcctcgggta
       61 tacatatttt caagacggga tactcttctg attccgtccg gtccgtctgc atatccggac
      121 tcaatccggc aagagaaatt cggacagaga gcggaagggt ggacggccgg acatcacttt
      181 ttctttcccg gggcttgctt cggacgtgcc gctttttttg ccatggcggc ggccccgctc
      241 tgggctatca ggatcgtggc gatctcgttg atctcgtcct ttgcgtcgag ctggcggatg
      301 gtcagttgct tgacggtttc tttggtcagg gagagggtgc tgtagatcgt tgcgtcaaac
      361 ggggcgggag cgcctcctgc aaagatgccg aacccggcca gctccgtgcc gtaatgtgcg
      421 aggtggatca gggagacgaa acgcttcaaa ttcgggtcgg aacaggcggc gggtgcatga
      481 tgacaggcga ctgcttcgat caggctggac ggcatgcccc actgggttgc gacaacacgg
      541 ccgatcgccg gatgtcccca ttcacccagg gatgccgctt cggcctcgac ataggatttt
      601 ccggaagcga caccatcaag gacccgctga aacatctcgg gaaagaaaga ttcgagaatg
      661 atgattccga aatcgtgaaa gagtgctgcc agatagaggt cccgtgtttc ctcctcggaa
      721 aacccgccct cggcttcggc aaattccttg gcggtcctgg ctgtagccag ggagtgtttc
      781 caaaaacggg cgcggtcaaa tttgactttt cctggctgac ggaatgagtc gataaacgcc
      841 gttgcgatca ggaggttgcg tgtcagggta tatcccaaca gggctgccgc ttcggagatg
      901 ctcgcgatcg gatcagggta cgcatagtag atcgtattga cgaccttaag gagtttggcg
      961 tcgagatctt cgttggaggc caggagcttt tgaagttcat atcctgccga atcggatgtt
     1021 tcgtcgatcg cgtttatgct cgagacaagc tgggggagtg cgggaatgtg ctcaaccgca
     1081 gccaagagtg cgtttttatc aaaatcggac atagtctttc cttgccaggg tacaggggaa
     1141 acagtcccac cgggactgtt tcgtatcata gcattccgcg agtgtatccg caaggcgcat
     1201 cgcgccggat tgacagtata ttttccggga tattgaaggc tctgaaggag gcagtgatgg
     1261 ggaatcctgt acgtggtgaa gatgccagtc tggttgttgt ggaccaggtt cttccgataa
     1321 aactccccct ggtgcccttg ttctcccggc cgctttttcc cgagatgctc agtccgatga
     1381 tgatcactga caagcactcg atcgaaacga tcgattacgc gctggcgcat ggcgggtatg
     1441 tcggtttcat tcttgtccgc gatccggaca aggaaccggg tgacaagggg aatttgcatg
     1501 atgtcggttc cgtggccaga atcctcaagc gggtccatct tccggatggc gggatcaata
     1561 ttttcgtaaa tgctgtcaag cgatttcgac ttcggaaatt gctagctgat gaaggttcga
     1621 tgctccttgc ggccgtgacc tacgagcacg aggaagagtc cgaggatgat gagaccaagg
     1681 ccttgaccag ggcgatcttc tccctcgtca gggagataac cgaaaacaat ccctttttta
     1741 ccgaagaagt ccgtatgaca ctggtcaacg tcaaaaaccc ggccagactc gccgatttca
     1801 tcgcatccat tctgaacatc tcccgggagg agcagcagac cgttctcgaa tcctgtgtcc
     1861 tcaaggagcg gctctccctg gtcctcaagc tcctgacccg tgaaaaagac ctcctcgagt
     1921 tgcagcacaa aatccaggac cagatcaacg agaagatttc caaacaacag cgggaattct
     1981 ttctcaagga gcagctcaag gccatccggc aggaactggg gatggaggtc gacgagaaga
     2041 ccggggatat tgcccgtttc cgggagcggc tgacagcgct tgcgctcgag ggtgaagcgg
     2101 gcgagaagct cgatgccgag atcaataaac tctcggcgac cgaccagcat tccccggaat
     2161 acacggttat ccgcaactat ctcgaaacgg cgcttgtcct tccctggaaa gaactgacgg
     2221 atgacagaat tgacctggag aaggccggga aactcctgtc ggcggagcac ttcggtctgg
     2281 atgaggtgaa aaagcggatt ctcgaattcc ttgcggtcca ggtcctgaaa aaggatgcgg
     2341 ccggttcgat tctctgtctt gtcggccccc ccggtgtggg aaagacgtca ttggggaaag
     2401 cgatcgccgc ctcgatgaac cgcaaattct tcagattctc gcttggcggg atgcgggatg
     2461 aggccgagat caagggccat cgccggacct atatcggggc catgcccggc aagatcatcg
     2521 aggccctcaa gatcaccaag acggccaacc cggttcttat gctggacgag atcgacaagc
     2581 tggggcagtc ctatcagggc gatccctctt cggccctgct tgaggtcctg gatcccgagc
     2641 agaatgccag tttcagggat cactatctcg atcttccttt tgatctctcg cgggtctttt
     2701 tcatcacgac ggccaatacg ctcgacacga ttcccgggcc actcctggac cggatggagg
     2761 tgatccgtct ttcggggtac atctccgagg acaaggcgca gatcgcctcg cgctatctga
     2821 ttccgcgggc gcttgaggcc aacggccttc ccaaaggaca tgtttccatc ggaaaatccg
     2881 ttctggtcga aatcgccaat ggctgggcgc gcgaggcagg ggtgcgcaat ctcgaaaaac
     2941 gcatcaaccg gatcatgcgc aagctcgcct gggagtacgt ctccgaagga aagatcgatc
     3001 cggtcacgat cacgagtgc
//
LOCUS       DAOSHE010000246         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31032_length_3002_cov_5.171361, whole genome shotgun sequence.
ACCESSION   DAOSHE010000246 DAOSHE010000000
VERSION     DAOSHE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31032_length_3002_cov_5.171361"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1371
                     /locus_tag="PLD82_07155"
     CDS             115..1371
                     /locus_tag="PLD82_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65198.1"
                     /translation="MYENISDLLQQIVLGEDSVLELKTVEFKGDKIIGPHKDGMADEL
                     AAFANTATGIIVLGVDDKTKEIQGLPSDKLDIVESWLRSICNDSIEPPLDCVIRKIRV
                     SDQNTSEKTIIRIDVPRSLFVHKSPNGYFRRIGSSRREMTPDLLARVFQQRSQVRLIR
                     FDEQPVPGSSLEDLDPTLWNKFRTVISDKGDLEFLQKMKLLVKTENGSLQATVSGILM
                     ASDAPESFLPSAFIQAVCYRGSERNAAYQLDAKDITGPLDVQIREACKFVERNMRVFA
                     IKAPNRIETPQFSMNAVFEAVVNAAAHRDYSIYGSKIRLHLFSDRLELFSPGAIPNTM
                     TIESISERQSARNELISSLLARCPMNVDATGSQRNYIMDKRGEGVPIIIIESEKLSGR
                     KPQYRLIDDSELLLTIFAAEPPPDEA"
     gene            complement(1735..2490)
                     /locus_tag="PLD82_07160"
     CDS             complement(1735..2490)
                     /locus_tag="PLD82_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOG65199.1"
                     /translation="MFKSVDHAVDGIIQNQLRSGKPLAVILAGHNGSGKSTLWYNKLA
                     DKLQIPLINADRMMLSILPEIKDRQPLPRWAVKLRDKNEAWMKVAQRGVLSFVDNAMV
                     SMVPFAMETVFSEWRIRPDGSIGSKIDLIEKLQVNGYYIVLLFVGLVNKELSILRVQS
                     RVLGGGHNVEVSKLRDRFPRTQHAIRKAVEIADASILVDNSGPPDDAQVVRVQERTDI
                     IFDIRQGATPAPSAAIREWLSVVCPEKEPEDAQ"
     gene            complement(2504..2737)
                     /locus_tag="PLD82_07165"
     CDS             complement(2504..2737)
                     /locus_tag="PLD82_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65200.1"
                     /translation="MRNIKAGQEALLRGRVVLKKKGIRLENTASRPIYYCDPSDVKLI
                     IREENGQIEKGRIVRGKFIPLTSSGHSQHLLTQ"
BASE COUNT          759 a          779 c          719 g          745 t
ORIGIN      
        1 tgggttggga accggtcgtc ccgctcgagg agggacttgt ttctacacta aaatattttt
       61 ccgcgctgat acagagtacg tgataccgtg atgacaaggc taacgggggc gccgatgtac
      121 gaaaatattt ccgacctgct gcaacagatt gtcttgggcg aagactctgt cctggaactc
      181 aaaaccgtag agttcaaagg cgacaagatc atcggaccac acaaagatgg aatggctgac
      241 gaactcgctg cctttgcgaa tacagcaaca ggtatcatag ttttaggagt tgatgacaag
      301 acaaaagaga ttcaggggtt accatcggac aaacttgaca tcgttgaaag ctggcttcgc
      361 tccatctgca acgattccat tgaacctcct ttggattgtg tgattcgaaa aattcgtgtc
      421 tccgatcaga ataccagcga aaaaaccata atacgcattg atgtgccccg cagcctcttt
      481 gtccacaaaa gccccaatgg gtatttccgt cgcatcggaa gctccaggcg agagatgaca
      541 ccggatcttt tagcaagagt tttccaacaa cgcagtcagg tacgactcat tcgcttcgac
      601 gaacaaccgg ttccagggtc ttctcttgaa gatcttgacc cgactttatg gaataaattc
      661 agaacagtca tctcggacaa gggagatctt gaatttctcc agaagatgaa actgctggtc
      721 aaaactgaaa acggatccct gcaggcaacc gtgagcggta ttctgatggc ttcggatgca
      781 ccggaatctt tcctccccag cgcctttatc caggctgtgt gctatcgtgg cagcgagcgg
      841 aacgccgcct atcaactgga tgcaaaggac atcactggcc ccctggatgt ccagatccgg
      901 gaggcctgca agtttgttga gcgaaatatg cgtgttttcg cgatcaaagc acccaatcgg
      961 atcgaaacac cacaattctc catgaatgcg gtctttgagg cagtggtcaa tgccgcagcc
     1021 catcgggatt actcgatcta cggctcaaag atccgtttgc acctcttctc tgaccggctg
     1081 gaactcttct caccgggcgc aatcccgaat acaatgacta ttgagagcat atcggaacgg
     1141 caatcagcac gaaacgaatt gatcagctca ttactggctc gttgcccaat gaatgtggat
     1201 gccactggca gtcagcgcaa ctatatcatg gacaaacggg gtgagggtgt gcccatcatc
     1261 atcatcgaaa gcgagaagct ctctggacga aaaccacagt atcgcttgat tgatgattcg
     1321 gagctgctct tgaccatatt tgcagccgaa ccgcccccag acgaagcgtg attgcatgac
     1381 agcgttcttc atcgcctgtc cagaatttct cccgatcgag gcgagatcgc catggcaaca
     1441 gccctaaaac tccaggaagg gggtcgtttg cgtattccga tgagcaacgg ggcgatcgtg
     1501 atgacgtcga atgatttttc gccggtgttg acatcggcag gggcgggttt gaaacctccc
     1561 tgcgtgttgc attgattcat tgcggttgat gcgttcgtaa tcattaccgg gcattcagtc
     1621 ggaaaaccga aggtcaggaa aagacgcttg acagcaacac agcgcgtcag cgcatactca
     1681 aaacgttcat ggcgtgtcgt tttgggtaaa ggatgacatc ctcaacaggg ttcattattg
     1741 agcatcctca ggttcttttt ccggacacac aactgataac cattcgcgga ttgctgctga
     1801 aggtgccggt gttgctccct gacggatatc gaatataata tcagtccgtt cctgcactct
     1861 gacaacctgt gcatcatccg gaggaccgct attgtcaaca aggatggacg catcggcaat
     1921 ttcgacagcc ttgcgtattg catgctgcgt ccgtggaaat cggtctcgca attttgatac
     1981 ttcaacattg tgcccaccac ccaaaacccg gctctgaaca cggaggatgg aaagctcctt
     2041 gttgaccaag ccaacaaaga gcagtacaat ataatacccg tttacttgga gcttctcaat
     2101 caggtcgatt ttgcttccaa tactgccgtc aggccttatc ctccattctg aaaaaaccgt
     2161 ctccatggca aatggcacca tactgaccat agcattatct acgaaagaca aaacacctct
     2221 ctgagcgacc ttcatccagg cctcgttttt gtcacgcagc ttgacagccc agcgtggcag
     2281 aggttgtctg tcttttattt ccggcagaat ggacaacatc atccggtcgg cattgatcaa
     2341 cggtatttgc aatttatcgg caagtttatt ataccaaagt gttgatttac ctgaaccatt
     2401 atgaccggca aggatcaccg ccaacggctt gccagaccga agctgattct ggataattcc
     2461 gtcgactgca tgatcaacac ttttgaacat accgaacaca gtttcattgg gtcagcagat
     2521 gttgactgtg accggacgac gtcagcggga tgaactttcc ccgaacaatc ctgccctttt
     2581 cgatttggcc gttttcctca cgaatgatca gcttgacatc ggaagggtcg caataataga
     2641 tcggtcggga agcggtgttt tcaagccgaa tccccttttt tttcaggaca actcgccccc
     2701 tgagcaaggc ctcctgcccg gctttaatat tccgcatgat gtcttccggc gcaggcgtat
     2761 aaatgccgat gcttgtcaaa ccgctcgctt ccctattcgg gaaaagacga ctcaaaagag
     2821 atcgcttctt tttcaatctg gtggctttgt ttttcatcat ggcatctctc ctgtcctatt
     2881 atacgcaaag ctgatatcca gcgcaacttt tttcgcgcaa cgtacgataa ccttgcccgc
     2941 catcatgtgc agcgtaaggg aaggtggaaa tgccgtggcg ttttatgccc gcgcatgcag
     3001 cc
//
LOCUS       DAOSHE010000247         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31094_length_2998_cov_3.556575, whole genome shotgun sequence.
ACCESSION   DAOSHE010000247 DAOSHE010000000
VERSION     DAOSHE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31094_length_2998_cov_3.556575"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLD82_07170"
     CDS             complement(<1..547)
                     /locus_tag="PLD82_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65201.1"
                     /translation="MRLPILTLLLLAGVVSSHPLIPVPGASRSLQHERWGDADGARPL
                     SYSAIRRALRLAAPDDAGGQRVMLVVDLARAEIIAASGLRPAFRQIHSPGSLLKLFTT
                     WYLLEAGRLAPDFRWRCQGTFILEQNDYPQDALDEQLHIGGGRYLYQAGCSLKNGHGK
                     IGISQAIGFSCNHFFRAVSGRI"
     gene            complement(563..1525)
                     /locus_tag="PLD82_07175"
     CDS             complement(563..1525)
                     /locus_tag="PLD82_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG65202.1"
                     /translation="MKRLVLAAAVIIAIIAVTGVGDPQPQSIINLIRSNASDEFIEAA
                     IRRRANVNERFVAFSRIYQGGYGWQGGSSSYLLYGGLTPLHMALFNNRSLALVNLLIN
                     SGADLKARDFDGNTVLHYAVANCTNQAIVTRLIQAGLSVQAVNRFGQTPVAYALNLSS
                     GYSRFMESTPANMDLRSRYGFWGSQEGVMTVAQHVKAIENNVVFLIPYGVANTQDKQG
                     RTLVMLAAMRGNNELLRRLIPAGPQGSALVNKKDYQGANALHLAVQNNGDSETITLLL
                     AAGADAKSSANGGTPFQLAIERYKSRGDHQLTPEILGRLSDLQY"
     gene            complement(1675..1911)
                     /locus_tag="PLD82_07180"
     CDS             complement(1675..1911)
                     /locus_tag="PLD82_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65203.1"
                     /translation="MRILLTVLMILAVLVTGCGYTEEDAVKEILAVPVVSNEQQQQAL
                     YNAVEEKCKKNNIDYVKALDKALRILKSQEKTGE"
     gene            complement(2036..2431)
                     /locus_tag="PLD82_07185"
     CDS             complement(2036..2431)
                     /locus_tag="PLD82_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HOG65204.1"
                     /translation="MRILFILALLMVGSVLTAYSSVAVYESNAGLIVGWSYNCDSQAD
                     ADATASQACREHGGRNPKIDLQSDGGWGALASGHDADGEPVWGYCLGASSREEAIQTA
                     IEQVKKAGAVSWKVEDAFLDVPSDAGDDE"
     gene            complement(2537..>2998)
                     /locus_tag="PLD82_07190"
     CDS             complement(2537..>2998)
                     /locus_tag="PLD82_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65205.1"
                     /translation="SANGQYSNPIADALLTKSWAEALGADNAISIPKLAFDKIPKANE
                     FVTLIVKSADTISALEQSGASTEFLAAVKKQFGDGALAFALVFEDKAQYENGKKVHLN
                     MGLFDVKMMTWKWITKTVYEAGMVPKPYEMVVQDLVDESFKALKEKAGSLR"
BASE COUNT          712 a          855 c          780 g          651 t
ORIGIN      
        1 cgatccgtcc cgacaccgcc cgaaaaaaat ggttgcagga aaatccgata gcctggctga
       61 tcccgatctt tccatgccca tttttgagcg aacacccggc ctgatagaga tagcgccctc
      121 ccccgatatg cagctgctcg tccagggcat cctgtggata gtcgttttgc tcgaggatga
      181 aggtgccctg acagcgccag cggaaatccg gcgcaagccg cccggcttcg agaagatacc
      241 aggtcgtgaa gagtttcaag agcgatcccg gggaatggat ctgacggaaa gcgggacgca
      301 agccactggc cgcgatgatc tcggcacggg ccagatcgac caccaacatg acacgctgac
      361 caccggcatc atccggagcc gccaggcgga gtgcccggcg gatggcactg tacgatagcg
      421 gccttgctcc atcagcgtca ccccaacgtt cgtgctgcag gctgcggctc gccccgggga
      481 caggaatcaa gggatgggaa gatacgacgc ccgccaaaag caataatgtc agaattggca
      541 ggcgcatctt catccataag agttaatact gcaggtccga caagcggcca agaatttcgg
      601 gggtaagctg atggtcgccg cgagatttat aacgctcaat ggcaagttga aatggcgtcc
      661 ctccgtttgc cgatgatttt gcatccgcac cggccgccag gagtaaggtt attgtttcac
      721 tatctccatt attctgcact gcaagatgga gcgcgtttgc accctggtaa tcctttttat
      781 tcaccaacgc tgatccctgc ggaccggcag ggatcagacg acgcaggagc tcgttattgc
      841 cccgcatggc agccagcatg acaagggtcc gcccctgctt gtcctgcgta ttggcgacgc
      901 cgtacggaat caaaaaaacg acattgtttt cgatggcctt gacgtgctgg gcgaccgtca
      961 tcacgccctc ctgcgatccc cagaatccgt agcgcgagcg cagatccata ttggccggcg
     1021 tcgactccat gaagcgggaa tacccgctgg agagattgag tgcgtacgcg accggagtct
     1081 gcccgaagcg gttaacggcc tgtaccgaga gcccggcctg gatcaggcgg gtgacgatgg
     1141 cctgattggt gcagttggcc acggcatagt gcaggaccgt attaccgtca aaatcgcgcg
     1201 ccttcaagtc ggcacccgaa ttgatcagga gattgaccag ggccaggctg cggttgttga
     1261 agagtgccat gtgcagcggg gtcagtccgc cgtacaaaag ataactggaa ctgccaccct
     1321 gccaaccata tccgccctgg tatatccggc tgaaagccac aaagcgttcg ttaacattgg
     1381 cccggcgccg aatggccgcc tcgataaatt cgtcactcgc attggaacgg atcaggttga
     1441 tgatactctg gggctgggga tcacccaccc ccgtaacggc aatgatggca ataatgacag
     1501 ccgcagcaag aacaagacgc ttcatacaga ctcctttggt ggacagtata aaaaagctag
     1561 cacaacaagc tcgtccagag aaacaatagc aggccgaatc ttcaagatga gctaatgttc
     1621 attttgtacc tcttaaatca cttctgtcca tatcattcac taaaaatctt ccaatcactc
     1681 cccggtcttc tcctgtgatt tgagaatgcg caaggccttg tccagcgcct tcacatagtc
     1741 gatattgttt tttttacact tttcttcaac tgcattgtac aacgcttgtt gttgctgttc
     1801 gttggagaca accggcacag ccaggatctc cttgaccgca tcctcctcgg tatagccaca
     1861 gcctgtcacg agtaccgcca aaatcatcaa aactgtcaat agaatacgca tactactctc
     1921 cttgtttata ttgggcgatg cacacatcgc catgaaaaat caccggccat aagcctgagg
     1981 tgcttcatca aaaaaaccat caggccaggc ttgcactcat gcatttacac aaaactcatt
     2041 catcatcacc ggcatcggac ggaacatcca aaaatgcatc ctcgaccttc caactgacgg
     2101 caccggcctt tttgacctgc tcgatggcag tttgtatggc ttcttccctc gatgatgctc
     2161 cgagacaata gccccagaca ggctcgccat ccgcatcatg tccactggcc agggcacccc
     2221 aaccaccatc actctgcaaa tcgatttttg gattccggcc accatgctcg cggcaagcct
     2281 ggctggcagt ggcatcggcg tcggcttgcg agtcgcagtt atacgaccag ccaacaatca
     2341 aaccggcgtt cgattcgtaa acggcaaccg acgaatacgc cgtgaggacc gatccgacca
     2401 taagcaatgc aagtatgaaa agaatacgca tgttgatgct cctttgattt gtacaattcc
     2461 gatggtgggt acatactcaa gcagggccga aagaatcccg aaatcctcga acaggactcc
     2521 gggacagatt cgaaccttac ctcaggctgc cggccttttc cttgagggcc ttgaaggact
     2581 cgtccacgag gtcctggacg accatttcgt agggtttggg aaccatgcct gcttcgtaga
     2641 cggtcttggt aatccacttc caggtcatca ttttgacatc aaaaagaccc atattgaggt
     2701 ggaccttttt cccgttttcg tactgggcct tgtcctcaaa gacgagggcg aaggccaggg
     2761 ctccgtcacc gaactgcttt ttgaccgcgg ccaaaaactc cgtcgaagca cccgactgct
     2821 cgagggcgga aatcgtgtcc gccgatttga cgatcagggt cacgaattcg ttggccttgg
     2881 gaatcttgtc gaaggccagc ttggggatgg agatggcgtt gtcggcaccc agggcctcgg
     2941 cccagctttt ggtcaagagg gcatcggcga tcgggttgga atactgtccg ttggctga
//
LOCUS       DAOSHE010000248         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31298_length_2984_cov_3.763400, whole genome shotgun sequence.
ACCESSION   DAOSHE010000248 DAOSHE010000000
VERSION     DAOSHE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31298_length_2984_cov_3.763400"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLD82_07195"
     CDS             <1..804
                     /locus_tag="PLD82_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG65206.1"
                     /translation="LPFHLRATYGGNTSCVYLNIDGTHIALDMGSGLRLLGLDLLTKT
                     FGRNSDEIHIFLSHTHWDHIQGLPFFVPAYLEGNRVHIYSPSENIADRLEMQQYAQFF
                     PISLREMGAEFNYERMELGKPYSIANFVVSSIKLNHPNNSYAYRFDHGGKSVVYATDT
                     EFNEQSIDFIKGCIEFFKNADLLIFDSQYTSKESFEKLHWGHSSANTGIDVAIKSGVK
                     RLVFFHHEPGYPDGQLSDIYKDIFRYRNMVARSRDLELIPGYEGLEITL"
     gene            complement(794..1840)
                     /locus_tag="PLD82_07200"
     CDS             complement(794..1840)
                     /locus_tag="PLD82_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65207.1"
                     /translation="MEILVSNRALMRCGMLRDGQACVLQEPVQVLKLMPGLVPDPGDL
                     FRVAGAQEKTEMLTGLLKGNDLTDDMLRELVLEGIIGASLLPDRVRVRALGPLKARPH
                     KQNSAWGRVVLSLIRAAWCRQIDSGSRLPAALKPAGDAFARVRSRAWYLWLVKGGAGH
                     LTRAVLKGYLPSWFGTVRLWAGIRPLVEGDVFGSFLEHRSRKWREEYAGFAGDAQDLA
                     HALAWLVQHGNELSRLLAVSGNHQSTYEFDELVARLADHGLLPSLLAETGFAGTVWIL
                     DSCSRETKRKIEEALAPGLKAACEGIWTGRLQGDFVNSDAHQKTARNRFFERGLLFLL
                     LAEDERVLQEIVRG"
     gene            complement(1929..2840)
                     /locus_tag="PLD82_07205"
     CDS             complement(1929..2840)
                     /locus_tag="PLD82_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65208.1"
                     /translation="MKVRHRLAVLGLACCLAVMTGCRTRETTRGRPASGAGGLKEGDG
                     YRLAGRITCVVKAESPLTVKVEATVRLAASGKIEKVVGSVASLKVTLDTFEIQYDKRF
                     ATILAPMIIRLKIPPTGTSLRFWIDRAQGDILSVQKTTEATKLTRAEAMLLGLLGFSG
                     RDEFQPVRISVEKPWPFELVYSGRTKETRDAYTTLTSRIRIKSPETPHPLGESVWVQY
                     FLSDLVPVRTTIQAEFTTRTRFPGGGIRVVVPLSWQIEGVLEYVKLRTLDSALTPPAW
                     FSRYRGSWFCWYPSVRKRCVAVCSDSQ"
BASE COUNT          700 a          879 c          778 g          627 t
ORIGIN      
        1 ctccccttcc atctccgggc aacctatggt ggaaacacat cctgtgtcta tctcaatatt
       61 gacggaacac acatcgccct cgacatggga agcggactcc ggcttctcgg gctggatctc
      121 ctgaccaaga cctttggccg caacagtgac gagatccaca tcttcctcag ccatacccat
      181 tgggaccata tccagggact cccttttttc gttccggcct atcttgaggg gaacagggta
      241 catatctatt ctccttccga aaatattgcc gaccggctcg agatgcagca gtacgcccag
      301 ttcttcccca tctcactccg ggagatgggg gccgaattca actacgaacg aatggaactc
      361 ggcaaaccct attcgattgc caattttgtt gtgagcagca tcaaacttaa tcaccccaac
      421 aactcgtacg cctaccggtt tgaccacggc ggaaagagcg tcgtctatgc gacggacact
      481 gagttcaacg aacagagtat cgatttcatc aagggttgta tcgaattttt caagaatgcc
      541 gatttgctga tttttgattc ccagtatacc tccaaggaat ctttcgaaaa actccactgg
      601 ggacactctt ctgcgaatac gggaatcgat gtcgcgatca agagcggcgt caagcggctt
      661 gtcttttttc accatgaacc gggttatccg gacggtcagc tttcggacat ctacaaagac
      721 atcttcagat accggaatat ggtcgcacgg agccgtgatc tggagctgat cccggggtac
      781 gagggactcg agattaccct ctgacaatct cctgaagaac ccgttcatcc tcagcgagca
      841 acaggaagag caggcccctc tcgaaaaaac ggtttctggc ggtcttctga tgggcatcgg
      901 aattgacgaa atccccttgg agacgtcccg tccaaattcc ctcacaggcg gctttgagcc
      961 cgggtgcaag cgcttcttcg atcttccgct tcgtctcgcg ggaacacgag tcgagaatcc
     1021 ataccgttcc ggcgaatccg gtttctgcca atagcgatgg cagcaggcca tgatcggcca
     1081 gacgcgctac cagttcatca aattcgtatg tgctctggtg gttgcctgag acagcaagca
     1141 ggcgggatag ctcattcccg tgctgtacaa gccaggccag tgcatgggcc aggtcttgtg
     1201 catcaccggc aaaaccggca tattcctccc gccatttccg tgaacgatgt tcgagaaagg
     1261 aaccgaagac atctccttcc actagaggac gaatcccggc ccaaaggcga accgtgccga
     1321 accaggaagg aaggtacccc ttgagaacag ctcgagtcag atgcccggca ccacccttca
     1381 ccagccagag ataccatgcc cgtgaacgca ctctggcaaa agcatccccg gccggtttga
     1441 gtgcagccgg aagacggcta cccgaatcga tctgtctgca ccaggcggcc cggatcaggg
     1501 agaggaccac gcgtccccat gcactgtttt gcttgtgcgg ccttgctttg agcggtccca
     1561 gggcccgcac cctgacacgg tcaggcaaaa gggatgcccc gatgatgcct tcaagaacaa
     1621 gttcacgaag catgtcgtcc gtcaggtcgt ttcccttgag gagtcctgtc agcatctccg
     1681 ttttttcctg tgctccggcc acacggaaaa gatctcccgg atcaggaacc aatcctggca
     1741 tcaacttgag tacctggacc ggttcctgaa gcacacaggc ctgcccatcc ctgagcatcc
     1801 cgcaacgcat gagggcccta ttggatacaa ggatctccat gctggcatga gtgatccgtg
     1861 cccccggatg gtcggactcg gggcagagtc tttggatatg atattcgaga tgatcaatat
     1921 cacttcggct actgggagtc agaacagacg gcaacacacc gttttcggac ggatggatac
     1981 caacagaacc aggatccccg atatcgggaa aaccaggcag gaggagtgag agcggaatcc
     2041 agggttctga gtttgacata ttccagaact ccctcgatct gccaggagag agggacaaca
     2101 acccggattc caccacccgg aaacctggtc cgggttgtaa attcggcctg aatcgtcgtc
     2161 cggacgggaa ccaagtcgga aaggaaatac tggacccaga cagactctcc aagcggatgg
     2221 ggcgtctcag gactcttgat cctgatccgg ctggtcaagg tcgtgtacgc atcccgtgtc
     2281 tccttggtcc tcccggaata gacgagttca aaaggccatg gtttttcgac agatatcctg
     2341 acaggctgga attcgtccct gccggaaaac cccagcaaac ccagcagcat tgcttctgcc
     2401 ctcgtgagct ttgtcgcttc ggtggttttt tgaaccgaga gaatgtcgcc ctgggctctg
     2461 tcaatccaga accggagact ggtcccggtc ggagggattt tgagacgaat gatcatgggg
     2521 gcaaggatcg tcgcaaaacg tttgtcgtat tgaatctcga aagtatcgag cgtgaccttg
     2581 agactggcca cgctgccgac aactttttct atcttcccgg aagcagccag tcgtactgtc
     2641 gcttcgactt tgaccgtcag aggcgattct gctttgacaa cacacgtgat ccggccggcc
     2701 agccggtacc cgtcgccctc cttcaggcca ccggcacccg aagcgggacg acctctggtt
     2761 gtctcccgag tccggcatcc cgtcatcacg gcgagacagc acgcaagacc gagtaccgcc
     2821 agtcgatgcc tgaccttcaa gcggaaacct catcaaacac gagactggca gcccccatca
     2881 ggccggcatc atttccgagg gaggcaggca gaatccggca gaccgtccct gcggccggca
     2941 agaccctaag acgaaaactc tcttccagcg gggtaaaaag aacc
//
LOCUS       DAOSHE010000249         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31348_length_2981_cov_3.241627, whole genome shotgun sequence.
ACCESSION   DAOSHE010000249 DAOSHE010000000
VERSION     DAOSHE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31348_length_2981_cov_3.241627"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..368)
                     /locus_tag="PLD82_07210"
     CDS             complement(165..368)
                     /locus_tag="PLD82_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65209.1"
                     /translation="MITDTIMVRVDVESIRTRISARNDCPAQDTDIILERVLLALQRQ
                     IEQEIREFEDGNGCFESCLFEAI"
     gene            complement(358..2028)
                     /locus_tag="PLD82_07215"
     CDS             complement(358..2028)
                     /locus_tag="PLD82_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65210.1"
                     /translation="MSDNEPIHETNDHGAMLNILEEKDKIIVSLKESSTQRDKINENQ
                     IRLLSEDNERKLGRINELTNSLQRLERTVSQRDAELAIYRERYSQYNERLEELRKAVG
                     EYKDSIAHALTEIRDMVTHELRDRDERGGQILEKLRDNQQKLEGKLSEVERYYKDIIH
                     QIAEKQVKAKLFIRKALVGLQEALNQLDLNPTQPMLDSRMIGEFSDLAARSDELHKEY
                     GNTSRDASTIIHNIENVHLQLSSTPKLDRFFREFGMATSESSRPGTEKPAEGSDTQAV
                     SGQEDGTQPGEPPANDVDRSGNGNTEDLPAEDLPGDGATNGIQADDLPGDLDSLLDSA
                     AREAGDGRAPAQGGNRAERGAPRLAQQPAPPAQPRSTGPQASQQHGSSQSIIQGGASF
                     MAPPPGIGGFAPVSGTGPYPGGPASQPFGSMPSGPMTAGTAALTVNSVPQGQQSFKTE
                     EDLILRESPNLAPYDWNMLPIASPLLHFRRMLNNAKKAEAQHNYIKAINIYRTVMEQK
                     TVQENDLARNILKDQLEALDRLARSQVSLNPKKHDIERYFGQEGRDDY"
     gene            complement(2131..2910)
                     /locus_tag="PLD82_07220"
     CDS             complement(2131..2910)
                     /locus_tag="PLD82_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65211.1"
                     /translation="MHATTRLAGFLLFLILAGCDTASLFERIETDDGRDWLCFAPGQT
                     RVYKMETNGALSPQTLTNHLVTTSVFGDTSHVVLSNITWLNRSFSQVFAWYPSARGWR
                     QHFNLLLALPPGNMEITASPARLDLPFLEKPWVAGNTVREERVQMISNFPLPPENIPV
                     TLMGVVSVTSRLEGTDLVMDAAGRRYADCLALRTITEIRLETTQANGPWSSGASVQSS
                     REELLIWLAPDTGIIRFELFSEERDIWNRRNRTSVKGELAN"
BASE COUNT          500 a          803 c          964 g          714 t
ORIGIN      
        1 gtccctgaac gacggcccct ccggaggcca gcgcggatgc gccgaagact gtcatgtgcc
       61 aggctgcacc cggaagcggg tccgcagcga tgtatcctga caggacaaac aggccgatga
      121 tgctgatgat tccacgtttc atgcgtaggg tgctccgctt gttttcagat ggcctcgaag
      181 aggcaggatt cgaaacatcc gttgccatct tcgaattccc gtatttcctg ctcgatctgg
      241 cgttgcaggg ccaggagaac tctctcaagg atgatgtctg tatcctgtgc cgggcaatca
      301 ttccgtgcag aaattcttgt gcggattgac tcgacatcca cccggaccat gatcgtgtca
      361 gtaatcatcc cggccctcct gtccgaagta gcgctcgata tcgtgtttct tcggattgag
      421 actgacctgg gatctggcca gacggtcgag ggcctcaagc tggtccttga ggatgtttcg
      481 tgccagatcg ttttcctgga cggttttttg ctccatgacc gttcggtaga tattgatggc
      541 cttgatatag ttgtgctggg cctccgcctt cttggcattg ttgagcatcc tgcggaaatg
      601 caggaggggg gaggcgatgg ggagcatgtt ccagtcgtag ggtgcgagat tgggactctc
      661 ccggaggatc aggtcttcct cggttttgaa ggactgttgt ccctgtggca cggagttgac
      721 cgtcagggcg gcggttccgg ccgtcatcgg tcctgacggc atcgaaccaa agggttgcga
      781 agcgggtccg cctggatacg ggccggttcc actcacgggg gcaaagccgc caattccggg
      841 agggggtgcc ataaaggacg ctccgccctg gatgatggac tggctcgagc cgtgttgctg
      901 gctggcctgt ggccccgtgc tgcgtggctg ggcagggggg gctggctgtt gcgccaggcg
      961 aggcgctccc cgctcggccc tgttgccacc ctgggcgggg gcgcgcccat caccggcttc
     1021 gcgggcggct gaatccagca aggaatccag atctcccgga agatcatctg cctgtatccc
     1081 gttcgtggct ccgtcaccgg ggaggtcttc tgccggaagg tcttcggtat tcccgtttcc
     1141 gctgcggtcg acatcgttgg ccgggggctc tccaggttgc gtaccatctt cctgtccgga
     1201 gaccgcctgg gtatcggacc cttctgccgg tttttccgtt ccgggacggc tggactcgga
     1261 tgtcgccatg ccgaattccc ggaaaaaccg gtccagcttg ggagttgaag aaagttgaag
     1321 atgaacattc tcgatattgt ggataatggt tgacgcgtcc cgggatgtgt tgccgtactc
     1381 tttgtgcaat tcgtcggaac gcgccgccag gtcggaaaat tccccaatca tgcgtgagtc
     1441 cagcatgggc tgtgtcgggt tcaggtcgag ctggttgagg gcctcctgca gcccgaccag
     1501 cgctttgcgg atgaagagct tggccttgac ctgcttttcc gcgatctggt ggatgatgtc
     1561 cttgtaatag cgttcaacct cgctcagctt cccttcaagt ttctgctggt tgtcacggag
     1621 tttttcgagg atttggccgc cgcgttcatc ccgatcccgg agttcatggg tgaccatgtc
     1681 acggatctcg gtcagggcgt gggcgatgct gtccttgtat tccccgacgg ccttgcggag
     1741 ttcttccagc cgttcgttgt actgggagta gcgttcccgg tagatggcca gttcggcatc
     1801 acgctgggag accgtacgct ccaggcgctg gagcgaattg gtgagttcgt tgatccttcc
     1861 gagtttgcgc tcattgtcct cggagaggag ccggatctgg ttctcgttga tcttgtcacg
     1921 ctgggtggat gattccttga ggctgacaat gatcttgtcc ttctcttcga ggatgttgag
     1981 catggccccg tgatcatttg tctcatggat cggttcgttg tcggacatgg cattccttcg
     2041 ggatcgtgta cccggaaatc cggccgggtg caccatggag gtattcactt catgtatcgg
     2101 cagacctggg cggtggctgt acgtttgcgc ttagttggcg agttcgccct tgacggaggt
     2161 ccggttgcgt ctgttccaga tgtcgcgttc ctcgctgaac agctcgaagc ggatgatccc
     2221 ggtgtcggga gcgagccaga tcagcagctc ttcccggctc gactggacac tcgcgccgct
     2281 gctccatggg ccattggcct gtgtggtttc cagcctgatt tcggtgatgg tccgcagtgc
     2341 gagacagtcc gcatagcgtc tgccggccgc atccatgacg agatccgttc cttcgagcct
     2401 gctggtcacg ctcacgacac ccatgagagt gacggggatg ttctccggcg ggaggggaaa
     2461 gttgctgatc atttggacgc gttcctctct taccgtgttg ccggcgaccc agggcttctc
     2521 aaggaagggg agatccagcc tggccggact ggcggtgatc tccatgttcc cggggggaag
     2581 ggccaacagg aggttgaagt gctggcgcca tccccgggcg ctgggatacc aggcaaagac
     2641 ttgtgagaag gaacggttca gccaagtaat gttggagagg acgacgtggg atgtatctcc
     2701 gaagacggat gtggtcacaa ggtggttggt gagcgtttgt ggggagagcg cgccgtttgt
     2761 ttccatcttg tagacccggg tctgcccggg ggcaaagcag agccagtcac gtccgtcatc
     2821 cgtctcaatc cgctcaaaga gactggcggt atcacagccg gcaagaatca ggaaaagcag
     2881 gaatcctgcc agacgggtgg tggcgtgcat gtgttttccc cttccggcta gagttggacg
     2941 gcggcaaaaa agccgattgt gatgaaggag ccgtcggcct c
//
LOCUS       DAOSHE010000250         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31558_length_2968_cov_4.399245, whole genome shotgun sequence.
ACCESSION   DAOSHE010000250 DAOSHE010000000
VERSION     DAOSHE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31558_length_2968_cov_4.399245"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLD82_07225"
     CDS             <1..1084
                     /locus_tag="PLD82_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65212.1"
                     /translation="DHSLLSLIPEQRPGVGREVLRRFGSRLALTADSILSLPESERNL
                     LLAAFVNSLHTLVPGQMTSLMPAGLQVSSLLSALLLDFWPVHAKACQFNIQAIHAGLQ
                     KKQPEFLVSGLNHLASGLRTLSRLPDDVGIFLRVKYAPVAALRPRDLAALTDKHDAPL
                     ALLTRLDSLHPLRDEPSPGLLAGLIAVLRLEHLKHLVTEEDREHTLNFSLLERFADCE
                     HVRKRFFSKPLPDPAPVLSEESIDSLRLKKRSLPEEPASAGADSEAVYFNRYDVLLAS
                     ERLLRLAIPKDRHCFGQLEILLARHGTGDGAALVSHAMRCERCSAYLSVSPLLEGGRE
                     RRLSGQTQRRMRSALSEQERVVTSSL"
     gene            complement(1106..2752)
                     /locus_tag="PLD82_07230"
     CDS             complement(1106..2752)
                     /locus_tag="PLD82_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65213.1"
                     /translation="MKRYLGHIFLTILSCACFVFVYYTASRGLLFFEDSRLSDQVAGI
                     ETILKVVEEDGKEILRRLNLLASDDVIIHTLAQYELGESLEKGALALRRLANGISGCE
                     SLRLIGIDDTIVIDSASDALEGKPLKDDEKQRMRVLLRKPGSFEFFADGRAEAVVRCL
                     SRAGMDVGILRVVLAPSFLTSRFYGGHCRASVVDGVVFLAEDGLLPKRDALTGLASEI
                     RRNMQEIRTSDAILVPVRSGFSSQVFAGISSRDGQGLPGIAGQILVLNGLVIVALVLL
                     LILRVINHWEHGRLELVRERIDAHESSMQEIQATAEDIIKATDTMYVTEEVEYHPEPE
                     THVPEPVDEPSDEMPTLSAVLARSRQTPVDQGEFETRGHDDELQTIIDRVAFQQDVIP
                     HEGENPEEDSVLAQDDSSGSGLDVWFEKIVAMLERSLDVKDVLLLENAGDGIYRVTQR
                     SGFSAACDSACTLAASEKLFTMFLARHKVLFIRESALSNPNMKKRFPDVDPDEISQLA
                     YIPVLKDGQILAILVFGCTGNGRGSLDQPALQEIQFLSML"
     gene            complement(2754..>2968)
                     /locus_tag="PLD82_07235"
     CDS             complement(2754..>2968)
                     /locus_tag="PLD82_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65214.1"
                     /translation="FSGALAGRGGQAIAGENGMFLAPGNVPVLLHTGDWAGGVERIRR
                     KRLALGVRSGYIVLFQGNWAQFIRVE"
BASE COUNT          636 a          918 c          803 g          611 t
ORIGIN      
        1 cgatcacagt ctgctttcgc tgattcccga gcaaagaccg ggggtcggca gggaggtctt
       61 gcggcgtttc ggttcccgcc tggcccttac ggccgattcg attctctctc ttcccgaatc
      121 cgagcgcaat cttttgctgg cggcctttgt caattcgctg cacacccttg tccccggcca
      181 gatgacgagc ctcatgccgg ccggcctcca ggtttccagc ctgctctcag cccttctgtt
      241 ggatttttgg cccgttcatg ccaaggcctg ccagttcaac atccaggcca tccatgccgg
      301 gctgcaaaaa aagcagccgg agtttctggt ttccgggctc aaccatctcg cctcgggact
      361 gcgcacgctc tcccgtcttc ccgatgatgt gggcatcttc ctgcgggtca agtacgctcc
      421 cgtcgcagcc ctccgtccgc gggatctggc ggctctgacc gacaagcatg atgcccccct
      481 cgcactcctg acacgactgg acagcttgca cccgctgcgt gatgagccat ccccgggact
      541 cctggccggt ctgattgccg tccttcgtct ggaacacctc aagcatcttg tcacggaaga
      601 ggaccgggaa cacacgctca atttctccct gctcgagcgt tttgcggact gtgaacatgt
      661 caggaagcgc tttttctcga aacccctgcc cgatccggca ccggttcttt cggaggagag
      721 catcgacagt ctcaggctga aaaaaaggtc tttgccggaa gagccagcct cggccggagc
      781 ggacagtgaa gccgtctact tcaaccggta cgatgtcctt ctcgcgtcgg agcgactctt
      841 gcgccttgcc atccccaaag acaggcactg ctttggccaa cttgagattc tcctcgcccg
      901 gcatggaaca ggagacggag cggccctggt ttcccatgcc atgcgctgcg agcgctgctc
      961 ggcgtatctc tcggtttccc ctctgctcga agggggaagg gagagacgtc tttcgggaca
     1021 gacacagcgc cgcatgcggt ccgctctcag cgaacaggaa cgagtggtca cctcatccct
     1081 ctgagggacg cggacatcca ccttctcaca acatggacaa aaactggatt tcctgcaggg
     1141 ccggttgatc caatgagccg cggccgtttc cggtgcatcc aaataccaga atggccagga
     1201 tctgtccgtc tttcaggacc ggtatatagg ccaattgact gatctcgtcg ggatcaacat
     1261 cgggaaagcg ctttttcata ttggggttgc tcaaggcgga ctcacggatg aagagaacct
     1321 tgtgcctggc cagaaacatc gtaaaaagct tttcggaggc tgccagggtg caggccgaat
     1381 cacaggcggc ggaaaacccg ctgcgttgtg tgacccggta gatcccatcc ccggcgttct
     1441 ccagcaggag gacatccttg acgtcgagag aacgctcaag catggcgacg attttttcaa
     1501 accatacatc aagcccgctc ccggaagagt catcttgggc caaaacggaa tcttcctcgg
     1561 gattttctcc ttcatgcgga atcacatcct gctgaaaagc gaccctgtca atgatcgtct
     1621 gcaactcatc atcatgtccc cgggtctcga actcgccctg atcgaccggg gtctggcggg
     1681 agcgagccag aacagcactg agggtcggca tctcgtcgga gggttcatcc acaggttcgg
     1741 gtacatgtgt ttcgggctcc ggatgatact cgacctcttc ggtgacgtac atagtatccg
     1801 tcgccttgat gatgtcctcg gccgtcgcct ggatctcctg catggaggat tcgtgagcat
     1861 caatccgctc gcggacgagt tcgagccggc catgctccca atggttgatc acccggagaa
     1921 tcaggaggag gaccagtgcc acgatgacga gtccattgag aaccaggatc tggccggcga
     1981 taccgggcag tccctgcccg tcccgggaag agattccggc aaagacctgc gaagagaagc
     2041 cgctccggac cggaaccaga atggcatccg aagtccggat ttcctgcata ttcctgcgaa
     2101 tctccgacgc cagaccggtc agtgcatcac gtttggggag caagccgtct tcggcgagga
     2161 aaacaacgcc gtcgacaacg gaggcacggc aatggccacc ataaaaacgg cttgtcaaaa
     2221 atgaaggagc gagcaccacc cggagaatac cgacatccat gcctgccctg gaaagacaac
     2281 ggacaacggc ttccgcccga ccatcggcaa aaaactcgaa actccccggc ttgcgcagga
     2341 gaacacgcat cctctgtttt tcgtcatcct tgagaggttt tccctcaaga gcatcactgg
     2401 cggaatcaat cacgatcgta tcgtcgatgc caatcagacg gagtgattca caaccggaga
     2461 tgccgtttgc cagtcggcgc aaggccaggg cgcccttttc gagagactcg cccaactcgt
     2521 actgggccag ggtatgaatg atcacatcat cgcttgccag aagattgaga cggcgcagga
     2581 tctctttccc gtcctcttcg acgaccttga gtattgtctc aatcccggcc acctgatcgg
     2641 aaagtctcga gtcctcaaag aagagaagac ccctgctggc cgtgtaatag acgaatacaa
     2701 agcaggcgca ggacaggatg gtcaagaaaa tgtgtcccag atatcgtttc acgtcattcc
     2761 accctgatga attgcgccca gtttccctgg aaaagaacaa tatatcccga tcgcaccccg
     2821 agtgcaagcc tcttgcgccg gatccgttca acaccaccgg cccagtctcc ggtgtgcagg
     2881 agaaccggaa cattcccggg tgcgaggaac atcccgttct cgccggcaat ggcctgccct
     2941 cctcttccgg ccaaggctcc agaaaagc
//
LOCUS       DAOSHE010000251         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_31606_length_2965_cov_4.690722, whole genome shotgun sequence.
ACCESSION   DAOSHE010000251 DAOSHE010000000
VERSION     DAOSHE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31606_length_2965_cov_4.690722"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PLD82_07240"
     CDS             <1..1405
                     /locus_tag="PLD82_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG65215.1"
                     /translation="PGGLCTAWKRKGYTLDYCIHWLMGTKKEGGFDRIWDELGALVNE
                     DGSPTGIVNFDEFMRVQLSDGETFKLYGKIDRLRDEMLRLAPEDAKMIARLCRDMRTL
                     SRLPFPGVIKEEWSLWRYLGFALANLGKIRIMLGYMNKRIGDFTAKWKSPRMREFFTA
                     IVPQDWSTLAFIFGLALQENQSAGYPLGGSLRFARNIERRALALGARIQYGKRVNSIL
                     VEQRSAVGIRLESGEIVRADHVVSAADGHATLFEMLDGKYLTPVQKKAYAGWPLFPSS
                     VFIGLGIGRDLTHLPRNCTLRLAQPFALPDGTEVTHIGVTVYHYDPEMAPAGKTAVTV
                     IINTWKGEFWKDFVRDNPAEYAAWKTAIGQEVVKTLDTTFGNIQDAVEVIDVSSPHTV
                     MRYTGNWQGSYEGFAPTPQAMKTRLPMQIKGLDHLYLIGQWSSAGGGLPPAGKGGRDV
                     AIAICRRDKKRFSGKMG"
     gene            1699..1941
                     /locus_tag="PLD82_07245"
     CDS             1699..1941
                     /locus_tag="PLD82_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOG65216.1"
                     /translation="MPREHKVIVYSTPTCSWCTKLKSWLREKRIRFVEVDVSKDARAA
                     EELVRKTGQMGVPVTLVDGRPVIGFNQPMLERLLAS"
     gene            complement(2041..2469)
                     /locus_tag="PLD82_07250"
     CDS             complement(2041..2469)
                     /locus_tag="PLD82_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65217.1"
                     /translation="MQTLSKLILSSILVFLVACHSEKPDTSGPVDIEQAALRAGINLT
                     NTPAASVKPEDLKDGERMTVVGMLREVGTARFSRLVITPLANYDIHLGIKKKQMEDYP
                     SLQHKFIEVTGVVTVQKVQFGTNQRNWYSMTVESARRVGK"
     gene            complement(2534..>2965)
                     /locus_tag="PLD82_07255"
     CDS             complement(2534..>2965)
                     /locus_tag="PLD82_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65218.1"
                     /translation="IRNGMPLLVWFGDDANYGLSYTFVQYCRLQTGDWNIMTELINHD
                     YGDYRALEAVVGQKVADLDSFDKLVKGYHLARLVNGSGIQGFKDENSTFRFTLRPPTD
                     SLDSFKLMPGGAIFLPVTEANLDGFVASGNGPQISFIKHKP"
BASE COUNT          680 a          819 c          828 g          638 t
ORIGIN      
        1 ccccggcgga ctctgcacgg cctggaaacg caagggctat accctggact actgcatcca
       61 ctggctgatg ggcacaaaaa aagagggtgg ctttgaccgg atctgggacg agctgggtgc
      121 gctcgtcaat gaagacggaa gcccgacagg gattgtcaat tttgacgagt tcatgcgcgt
      181 gcagctctcc gatggcgaga ccttcaaatt gtatggcaag atcgaccgcc tgcgtgacga
      241 gatgctgcgc ctggcccccg aggacgcgaa aatgatcgcc cgcctctgcc gcgatatgcg
      301 cacactttcg cggctgccct ttccgggtgt gatcaaggaa gaatggagtc tctggcgcta
      361 tctcggcttt gcccttgcaa acctgggaaa aattcgcatc atgctgggct atatgaacaa
      421 acggatcggt gattttaccg ccaaatggaa gagcccccgc atgcgcgaat tttttacggc
      481 cattgttccg caggactggt caaccctggc cttcattttt ggcctggccc tgcaggaaaa
      541 tcagagcgcc ggctatcctc tgggcgggtc actgcgcttt gcccgcaata tcgagcgccg
      601 ggcactcgcg cttggagccc ggattcagta tggaaaacgg gtcaacagca ttcttgtgga
      661 acagcgttcg gccgtcggga tccggcttga aagtggagag attgtgcggg ccgatcacgt
      721 cgtctcggcg gcagatggcc atgccaccct gtttgagatg ctcgacggta agtacctcac
      781 cccggtgcaa aaaaaggcct acgcgggctg gcccctcttc ccctcctccg tctttatcgg
      841 cctggggatc ggcagggacc tcacacacct gccgcgcaat tgcaccctgc gactggcaca
      901 gccgtttgcc ctgcccgacg gaacagaggt gacccacatc ggtgtcacgg tctaccacta
      961 cgatcccgaa atggcccctg cgggaaagac ggccgtcacg gtcatcatca acacatggaa
     1021 gggcgagttt tggaaagact ttgtgcgtga caatcccgcc gaatatgctg cctggaaaac
     1081 cgccatcggg caggaagtgg tcaaaaccct tgatacaacc tttgggaata tacaggatgc
     1141 cgtcgaagtg atcgacgtct cctcgccaca taccgtcatg cgctacaccg gcaactggca
     1201 gggaagttac gagggttttg cccccacgcc gcaggccatg aagacccggc tccccatgca
     1261 gatcaagggc ctcgaccatc tctacctgat cgggcagtgg agctcggccg gcggcggtct
     1321 cccgcccgcg ggcaagggcg ggcgcgatgt cgccatcgcc atctgccgcc gcgacaaaaa
     1381 acggtttagc gggaagatgg ggtgaagctc tctataaggc atgtgggcgc ttcactttcc
     1441 ctttggatac aacccaagag cggaaccatt gaagcagttc cccgacacaa tgagatcaaa
     1501 aagcgcctct tgccaaaaaa gatcatgaaa aatctttcct gtgaagaatt cccgaactgg
     1561 tattcacgtc gtatcccgtc ttgcactgtg gccttgtcac caggagcaca atggtggaca
     1621 cagccctgtc gggcaggaaa cctcttgatt ttgctgttgt tgtatgtata tttttatata
     1681 tacagaagga gccaatctat gccgcgagaa cacaaagtga ttgtctattc gacacccacg
     1741 tgcagttggt gcaccaagct caaatcctgg ctgcgggaaa aacggatccg ctttgtcgag
     1801 gttgatgtct caaaggacgc ccgggctgcc gaggaattgg tgcgaaaaac cggacaaatg
     1861 ggcgtcccgg tcaccctcgt tgatggccga ccggtcatcg gtttcaatca gccaatgctg
     1921 gaacgactcc tggcatcttg aagccggcat gagaccccgg cttcattgca tttgtacgca
     1981 ccacccacag ggattcgggc agaagtatca accgtccaga cactccctgg aaataccggg
     2041 ttattttccg acacgtcgcg cagattcgac ggtcatcgaa taccagttcc gctggtttgt
     2101 cccgaactgg actttttgga ccgtcactac tccggtaact tcgatgaatt tgtgctgcag
     2161 actcggatag tcctccatct gctttttttt gatgccgaga tggatatcat aattggccag
     2221 cggtgtgatc accagcctcg aaaagcgggc cgtccccact tcacgaagca tcccgacaac
     2281 agtcattcgc tcgccatcct tgaggtcttc gggcttgacg gaggccgcag gagtatttgt
     2341 gagattgatg ccggccctga gggccgcctg ttcgatatcg accggaccgc ttgtgtccgg
     2401 tttttcactg tgacaggcaa caaggaaaac aaggattgag gagagtatca gttttgacaa
     2461 ggtttgcata ggttctccag agagggtaca aggaaatcaa accgcctgcc ggatgagggc
     2521 aggcgggacc ggattatggt ttatgcttga taaaactgat ctggggtcca ttgccactgg
     2581 caacaaatcc atccagattc gcttcggtca caggcaggaa gatggcacca ccgggcatga
     2641 gcttgaagga atcaagcgaa tcggttggag ggcgcaaagt gaaacggaaa gtgctgtttt
     2701 cgtccttgaa gccctgaatc cctgagccgt tgaccagacg ggcgagatga taacccttga
     2761 cgagtttgtc aaaggaatca agatcggcta ccttttgccc gacaacggcc tcgagtgccc
     2821 ggtaatcacc atagtcatgg ttaatgagct cggtcatgat gttccagtct cccgtttgca
     2881 gacggcaata ctgcacaaag gtgtacgaga gtccgtaatt ggcatcatcg ccaaaccaga
     2941 caaggagggg catcccgttg cggat
//
LOCUS       DAOSHE010000252         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32177_length_2929_cov_3.914057, whole genome shotgun sequence.
ACCESSION   DAOSHE010000252 DAOSHE010000000
VERSION     DAOSHE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32177_length_2929_cov_3.914057"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1494
                     /locus_tag="PLD82_07260"
     CDS             77..>1494
                     /locus_tag="PLD82_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG65219.1"
                     /translation="MLESVRNQSIDIMFAMRTSEREKIYLFGTPFLEISVRIFVREDV
                     FGISGLPDLENRTVAVVRGFACQDALIESGLDFRIVAVETVAQALDLLANGDVFAFVG
                     QYHLAKYALQQKARQVKTIGDPLQTTPFGIAMHREQQNGISRLDRALEELKKTGEYAK
                     IHEKWYGTSSGPDTQTVLRYSLPILIGLLAIFGILVTWSLTLRHQVRVRTSALYENLE
                     KFRKLTDRAPFPVAIIHDNSIEYINPQFTMTFGYTLDDIPDCEAWFSTVFPDPAYRAT
                     VIADWQQHVTEKIQINDTPRTYDVTTRDGRIVTVDFRMIHLDDGRIIIILEDVTLKHR
                     MEEERLRQQQIESISLLAGGIAHDFNNILMHLIGHINLLQISEGMAEEQMESLSSMER
                     SIQRARGITGQLLTFSRGGAPVKKLQSLAPIIRESIDFVFHGSNCTAEVAVPDNLPAL
                     DIDSGQISQTLNNILINAIQAMP"
     assembly_gap    1495..1594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1595..1821
                     /locus_tag="PLD82_07265"
     CDS             <1595..1821
                     /locus_tag="PLD82_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65220.1"
                     /translation="VITDLTVPGGMGGREAISILRNAVPSIKAIVSSGFSCDPVLADC
                     TAWGFVAALVKPYTFSQLVQILASVLNQPS"
     gene            1879..>2929
                     /locus_tag="PLD82_07270"
     CDS             1879..>2929
                     /locus_tag="PLD82_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65221.1"
                     /translation="MRILVMDDSDSLGPALEAALSTAFRSVTIDTIRADSLGFSGCSA
                     CDVCSWKTPGICAHTGPARDVAKLFVRADICIITTRLLHGTPRASAKALWEHILPAVH
                     PGFVRYRGELHHRLRYPRLPQLACLAEGNHSRHDPLSQIESAIFAGWAERAACNTMSY
                     AAALCIPRGQSADPGLIATLLLDCANQSTMPARGGTLPPLAVPGLPAPSDPGAISPLL
                     ILAASGRKNSNSLAIAHWLAQRTRQTGLETLVIDAMTVVPGQLVRSMEQTGHLFVVVP
                     TWHDALPAATIRLFGQLASDGNLAGRGRPLSALVHSGYPEPAQTATALASLRLLALEN
                     GWLWAGGLTAGATSVI"
BASE COUNT          596 a          919 c          735 g          579 t
ORIGIN      
        1 caagcacatt cgtccattcc aggaatcgga actcgggtcg gtccccggcg agttccgatt
       61 cctggaatgg acgaatgtgc ttgaatccgt ccgcaaccag agtatcgaca tcatgtttgc
      121 catgagaacg tccgaacggg aaaaaattta cctcttcgga actccctttc tcgaaatctc
      181 agtccggatt ttcgtcaggg aagatgtctt cggaatctcc ggcctgcccg atctcgagaa
      241 ccggacagtg gcggtcgtac gcggatttgc ctgccaggat gccctgatcg aaagcggact
      301 cgactttcgg attgtcgctg tcgaaaccgt cgcccaggca ctcgacctcc tggccaacgg
      361 agatgtattc gcctttgtcg gtcagtatca tcttgcaaaa tacgccttgc agcaaaaagc
      421 ccggcaggtc aagacgatcg gagacccgct ccagacaacg cccttcggga tcgccatgca
      481 tcgggaacag caaaacggca tcagccggct cgaccgggca cttgaggaac tgaaaaaaac
      541 cggagaatac gccaagatcc atgaaaaatg gtatggaacc agttccgggc cggacacaca
      601 gacagtcttg cgctactccc tgcccatcct gatcggcctg cttgccatct tcggcatcct
      661 tgtcacttgg agtctcaccc tgcgccacca ggtccgcgtc cggacaagcg ccctctacga
      721 gaatctggag aagttccgca aattgacaga ccgtgcgccc tttcccgtcg ccattatcca
      781 cgacaacagt atcgaataca tcaatcctca gtttaccatg acctttggat acaccctcga
      841 tgacatcccg gattgcgagg cttggttcag caccgtcttc cctgatccgg cgtaccgtgc
      901 cacggttatc gcggactggc aacagcatgt cacggaaaaa atccagatca atgacacccc
      961 gagaacctac gacgtaacca cacgcgacgg ccggattgtc acggttgatt tccgcatgat
     1021 ccatcttgat gacggccgga tcatcatcat ccttgaagac gtaaccctca aacaccgcat
     1081 ggaggaggaa cgtctccgac agcagcagat cgagtccatc tccctgcttg cggggggcat
     1141 tgcgcatgac ttcaacaata tcctcatgca cctcatcggt cacatcaacc ttctgcagat
     1201 cagtgaaggc atggcggaag agcagatgga gagcctctcc tccatggaac ggtccatcca
     1261 gcgggcacgg gggatcaccg gacaacttct gactttttcc cggggaggcg ctccggtcaa
     1321 aaaattgcag tctctcgccc ccatcatcag ggaaagcatc gactttgtct tccacggctc
     1381 taactgcacg gctgaagttg ctgttcccga caacctgccc gctctcgaca tcgacagcgg
     1441 ccagatctcg caaaccctca acaacatcct gatcaacgcg atccaggcca tgccnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgtca tcacggattt gaccgtcccg
     1621 ggaggcatgg gcggacgcga agccatctcc atccttcgga atgcggtccc ttccatcaag
     1681 gccatcgttt ccagcggttt ttcctgcgac cccgtcctcg cagactgcac agcatggggc
     1741 tttgtcgcag ccctggtcaa gccgtatacg ttttcacaac ttgtgcaaat tctggcatcg
     1801 gttttgaatc agccatcctg aattccgttc ccggtaattc tttcgtactt ttgacggcag
     1861 cgggaggtga cgacagcaat gcgcatactg gtaatggacg attcagattc tctcggaccg
     1921 gcgctggaag cagctctctc aacagctttc cggtccgtaa ccatcgatac catccgggcg
     1981 gactccctcg gcttcagcgg ttgctcggcc tgtgacgtct gctcctggaa gacacccggc
     2041 atctgtgccc acaccggacc ggcgagggat gtcgccaagc tctttgtccg ggccgatatc
     2101 tgcatcatca cgacgcggct gttgcatgga acgccgcggg ccagtgcgaa agcactctgg
     2161 gaacacatcc tgccggctgt gcatccaggt tttgtccgct atcgcggcga actgcaccac
     2221 cgcctgcgct acccgcggct cccgcaactc gcatgtcttg ccgaaggcaa ccactcccgg
     2281 cacgatccac tctcgcagat tgaatccgct atttttgcgg gctgggccga acgggccgcc
     2341 tgcaatacca tgtcatacgc ggccgccctt tgcatccccc gcggacagtc cgcggatccc
     2401 ggactgattg caaccctgct gctggattgc gccaaccaga gcaccatgcc tgccaggggt
     2461 ggcacgctgc cgccgttggc agtccccggt ctgcccgcac cctccgatcc gggagcaata
     2521 tctccgctcc tgattctggc agccagcggc cgaaaaaaca gcaacagcct ggcaatcgca
     2581 cactggcttg cacaacggac caggcagacc ggtcttgaga ctctcgtcat cgatgccatg
     2641 accgtcgtac caggacaatt ggtccggagc atggagcaaa ccggccatct gtttgtggtt
     2701 gttccgacct ggcacgatgc attgccggcc gccaccatcc ggcttttcgg acagcttgcg
     2761 tcggacggaa acctcgccgg acgtggtcgc ccgctgtcgg ctctcgtcca ctcgggatac
     2821 cccgaacctg cccagacagc gaccgccctc gcatcactca gactgctcgc gctcgagaac
     2881 ggctggctct gggccggtgg attgacagcc ggggcgacgt cggtcatcg
//
LOCUS       DAOSHE010000253         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32181_length_2929_cov_3.704941, whole genome shotgun sequence.
ACCESSION   DAOSHE010000253 DAOSHE010000000
VERSION     DAOSHE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32181_length_2929_cov_3.704941"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLD82_07275"
     CDS             <1..670
                     /locus_tag="PLD82_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65222.1"
                     /translation="GDFGTTARDLNGNESGYFPLTRRDITRYAFKRVILGHIHKPSSL
                     DDEVLVPGSPWPIDSSERGPRRVLVLETLDGSVTSLGLGFSPCWLDWTIHLLPDGNEP
                     ARIKEALDHNFLHWERSQADGGQTGPVHVRVSVRGCTSRRSEPDRIVQQWFAGTPLQL
                     AECTSDDLWFVETDSSLKLLTAEFEQALAELKLDPRQFPGDHNEMLDMIREYGLSYIY
                     GLEE"
     gene            674..2746
                     /locus_tag="PLD82_07280"
     CDS             674..2746
                     /locus_tag="PLD82_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG65223.1"
                     /translation="MGMMIRTIDLRNAGPIQRFTHTLAPLTLIYGHNESGKTTILENL
                     VRAIFGGKKSRLKNLAVRGDDFEGSIKLTLERDGQQIALTNGLEAVLQKEETSLPADL
                     FRLLYVRAGDSALGDGKKGLSRELVKGLVSNQQTWDRIRQAIPGEIGYTRFADGVIEG
                     DRRGKMKQLVASRERLAEIRQLTDEFYATLSRAGIDRLRREEKILLDRLAEQEIARRH
                     EAWLLAKKKAKEEGLVRLIDKNRLARLERLLDEYRTLAARSGELAKRIRQVQPAEAGL
                     AWLKSAREHWLEFVRTGQSRSPAWPGYLAIGALVLSLAGMGLSRIAGVIFGIVAAAAL
                     VVKLVMDRRSEKTAGPVAELSALSAECHKRFGFEPKTVADLDAHGERLRDDAALFRTM
                     KQEQDGVEQRLAEVTAELKQAFPDVASQNWGESLDALRARLAKHEETCRKYELDLARL
                     GVDELDWLEEGNGIPYSGAQVHKLETGLDAIRDELKKEEEVLRTVQLHAVRLLQQQLP
                     TNHEQAALLLEEARREAERDVRKTFAKLAAGHLVEQVLQRFLDEEDRRVESWMNDAGV
                     GKLVHAVTGRYNRLAFEENELVVANKTERFALSSLSSGATEQVLLGVRMGIAQAVTGE
                     ETLFLLLDDAFQLSDWKRRGMLVTALGRMLDQGWQVVYFTMDDDIRQRMAALGKTRKT
                     GEYCEIVL"
BASE COUNT          635 a          872 c          915 g          507 t
ORIGIN      
        1 tggggatttc gggacgacgg cccgtgacct caacggcaac gagtccggct attttcccct
       61 gaccagacgc gacatcaccc gatacgcctt caagcgtgtc atcctcggtc acattcacaa
      121 gccgtcctcc ctcgatgacg aggtccttgt gcccggttct ccctggccga tcgattccag
      181 cgaacgcggg ccgcgccggg tcttggtact cgagaccctg gacggctccg tcacctccct
      241 tgggctcggg ttttcaccct gctggctgga ctggaccatc catctcctcc ccgacggaaa
      301 cgaacctgcc cggatcaaag aggcgctcga tcacaatttt cttcactggg aacgcagtca
      361 ggccgacgga gggcagacgg gcccggttca tgtgcgtgtc agcgtgcggg gctgcacctc
      421 ccgacgaagt gaaccggacc gcatcgtgca acaatggttt gccggtacgc ccctccagct
      481 tgcggaatgt acaagtgatg acctctggtt tgtcgagacg gacagcagcc tcaagctcct
      541 gacggccgag ttcgaacaag ctctggcgga actcaagctc gacccccgcc agtttcctgg
      601 tgatcacaac gagatgctgg acatgatcag ggaatacggg ttgtcctata tctacgggct
      661 cgaggagtaa aacatgggaa tgatgatccg cacaatcgac ctgcgcaatg cgggcccgat
      721 ccagagattc acgcacaccc ttgcgcccct gaccctgatc tacgggcaca acgaaagcgg
      781 gaaaacgacc atcctcgaaa accttgtccg ggccatcttc ggggggaaaa aaagccggtt
      841 gaaaaatctc gccgtgcgag gcgacgattt cgagggaagc atcaaactga ccctcgaacg
      901 ggacggccaa cagatcgcgc tcacaaacgg gctggaggct gttctccaaa aagaagaaac
      961 aagcctgccc gcagacttgt tccggctcct ctatgtccgc gccggggatt ccgctttggg
     1021 cgacgggaaa aaggggctca gtcgtgaact ggtcaagggc ctggtctcga accagcagac
     1081 ctgggaccgt atccgtcaag cgattcccgg cgagatcggg tatacccggt tcgcggatgg
     1141 cgtgatcgag ggagatcgcc ggggcaagat gaaacaactc gtcgcaagcc gggaacgact
     1201 ggcagaaatc aggcaattga ccgacgaatt ttatgcaacc ctctcccgcg ccgggattga
     1261 ccggctgcgt cgcgaagaaa agattctgct tgaccggctt gcggaacagg agatcgcccg
     1321 ccggcatgag gcctggctcc tggcgaagaa aaaggccaag gaggaagggc ttgtccgttt
     1381 gatcgacaaa aaccggctcg cccgactcga gcgcctcctc gacgaatacc ggacactcgc
     1441 ggcccgctcc ggtgagttgg ccaaacggat ccgtcaggtc cagccggccg aggcgggact
     1501 ggcctggctc aaaagcgcac gcgaacactg gctcgaattt gtccggacgg ggcagagccg
     1561 gagcccggcc tggccgggat acctcgccat cggagcgctg gtcctgtcat tggccgggat
     1621 gggactctcc cggattgccg gggtcatctt cgggatcgtg gccgccgccg cccttgtcgt
     1681 caaactggtc atggacaggc ggtccgaaaa gaccgcgggt ccggtcgctg aactctccgc
     1741 cctgagtgcg gaatgccaca agcgcttcgg cttcgagcca aagacggttg ccgatcttga
     1801 tgcacacggg gagcgcctgc gcgacgatgc ggccctcttc cggaccatga agcaggagca
     1861 ggatggcgtg gaacagcggt tggcggaggt gacagccgaa ctcaagcagg cattccctga
     1921 tgtggccagt cagaactggg gggaatccct cgatgccctg cgggcgaggc tggcaaaaca
     1981 cgaagagacc tgtcgcaagt acgaactgga cctggcccgg cttggcgttg acgaactgga
     2041 ctggctcgaa gagggcaatg gaatccccta ttcaggcgcc caggtgcaca agctcgagac
     2101 cggactggat gcgatccggg acgagctcaa aaaggaagaa gaagtcctgc ggactgtcca
     2161 gctgcatgcc gtcagactcc tgcagcaaca gctcccgacc aatcatgagc aggcggcctt
     2221 gttgctggaa gaggcccggc gcgaggccga gcgggacgta cgcaaaacct tcgcaaaact
     2281 ggctgccggg catcttgtcg aacaggtcct gcagcggttt ctggacgaag aggacaggcg
     2341 ggtcgaatcc tggatgaacg atgccggcgt ggggaagctg gtccacgccg tgaccggacg
     2401 ctacaaccgg ctcgccttcg aggagaacga actcgttgtg gccaacaaaa ccgagcgctt
     2461 tgcgctttcg tcactctcaa gcggggcgac cgaacaggtc ctgctcgggg tgcggatggg
     2521 aatagcccag gccgtcaccg gcgaagagac cctctttctc ctgctggatg atgccttcca
     2581 gttgtcggac tggaagcggc gcgggatgct ggtgacggca ctggggcgga tgctcgacca
     2641 aggctggcag gtggtctatt ttacgatgga cgacgacatc cgccagcgga tggccgccct
     2701 cgggaagacg cgcaagaccg gagaatattg cgaaatcgtg ctgtagcact tgagcatggg
     2761 gcagaaaagt attacaaaat cagcatggga ttccgggata cgcatgacaa acacgcaatg
     2821 ttgtcatggg ataacgggtg agaaacgaaa agcgtttccc tctcccaacc tccccctccg
     2881 gctcaggatc gggcccactc cagggagtcc cgcttcgccc gatagtggt
//
LOCUS       DAOSHE010000254         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32227_length_2927_cov_3.274373, whole genome shotgun sequence.
ACCESSION   DAOSHE010000254 DAOSHE010000000
VERSION     DAOSHE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32227_length_2927_cov_3.274373"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLD82_07285"
     CDS             complement(<1..325)
                     /locus_tag="PLD82_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65224.1"
                     /translation="MKAAIIKEEDRIDKILHRVKSDSRRITVRSGEKETHAAITTIDH
                     RTLQLSPNGVFVPESTQLTISFMIGGDSYVGHVTILSSAGGYLIVDKPDSLENRVVRR
                     TARIFV"
     gene            complement(322..624)
                     /locus_tag="PLD82_07290"
     CDS             complement(322..624)
                     /locus_tag="PLD82_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOG65225.1"
                     /translation="MREIARILSSLKSDEIEIFLEQLLTDTEQDMIRRRWELVRLLHQ
                     GVPQRDIASRLSMSLCKVTRGAKELKRPDSVFRKILEEKQVVPETGDTQNSGGVPP"
     gene            773..2260
                     /locus_tag="PLD82_07295"
     CDS             773..2260
                     /locus_tag="PLD82_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65226.1"
                     /translation="MAVPQNRISLEKVLVALYDRIIAREITQISQVVDQTKSIPLDAQ
                     QRETMMQKAFFLIKTLNWGVFADLNVREYTVLWHELVLSHPSYKFAGDLKRSMTISNV
                     FISMLDIHGYTAFCRQSKNNLSQLHELDNFLNTTIRDVAKNNGCIGNRERGDEIVLVG
                     GRASDILKATFEIIQIFARENFFKQSTNADPSDKYNRILPAFNISAGISGGNTNSPMI
                     ITRKGEISGFLLNTAARLQTRANKLAPKHTKIIIAKTVHTYLEKENRLQDFMKYIDFF
                     DCGSIEFKGMSIANMEVLFREADKKKLKYQKVFLEMLSSLRQNLWKDRVMLDCLSTMD
                     AVITILPPMRMEVALPGYKTLAISNDLLHRKITIIQNSFSIQDDYITAIIKFDELMVI
                     LQQVPDFEPIVLEYGQEINDRYKMILTHYEPELEREIEQKKGMIFSPQELRVYDYFKS
                     NANSLELLAEKARKSPQLSQRKILWNSMIEKKKSELDFSLYSGKK"
     gene            complement(2332..>2927)
                     /locus_tag="PLD82_07300"
     CDS             complement(2332..>2927)
                     /locus_tag="PLD82_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG65227.1"
                     /translation="GDSRAELEKSPYLEAVRKKSYEVLFFTDPVDEWILPQMPEYKGK
                     KFLSLAKGDLPEGTEEEKKTQEKQREDLKGLVAWMGKRFEDKIKEVRVSSRLVTSPSC
                     LVADEYDMGAHMEQLMKAMRQDVPKAKRILEINPGHALVMKLNQLQADESRSDLAGEI
                     ADALYANAVIAEGGKIEDPASFGEFFSKLLLEHAERS"
BASE COUNT          723 a          780 c          702 g          722 t
ORIGIN      
        1 gaacaaaaat ccgggcggtc cgtcgcacaa cgcggttttc gagagaatcc ggcttgtcaa
       61 cgatcagata accgccggca ctggagagaa tcgtgacatg gccgacgtat gaatctcccc
      121 cgatcatgaa acttattgtc aactgtgtac tctcgggaac aaagacgcca ttcggcgaga
      181 gctggagcgt gcgatggtca atggtggtga tcgcggcgtg ggtttccttc tcacctgacc
      241 tgacggtgat ccgtcgggag tcggatttga ccctgtggag gatcttgtcg atccggtctt
      301 cttccttgat gatggcagcc ttcacggggg tactccacct gaattttgtg tatcacctgt
      361 ctccggaaca acctgttttt cttcaaggat tttccggaat accgaatcgg ggcgtttgag
      421 ctctttagcg cctctggtga ccttgcagag actcatggaa agtctggatg cgatatcccg
      481 ctgagggaca ccctgatgga ggagccggac cagttcccag cgtctccgga tcatgtcctg
      541 ttcggtatcg gttaaaagct gttcaagaaa aatttcgatc tcatccgact tgagggaaga
      601 aaggatccgg gcaatctcgc gcatgtctgg tctctcactc cacttgctgg attcaacatt
      661 gtgtatagta tcacagaaca gagccaagtc ggaaattttt tcaatcgaca aagttgacgg
      721 tttattctat actcttgtta ccatctgcat tgacggaaca agagaggcaa tcatggcggt
      781 tccccagaac agaatttcac tggagaaggt tcttgttgcc ctgtatgaca ggatcattgc
      841 ccgtgagatc acccagatct cccaggttgt cgatcagacc aagagtattc ccctcgatgc
      901 ccagcaacgc gaaaccatga tgcaaaaggc attttttctc atcaaaaccc tgaattgggg
      961 tgtttttgcc gatctgaatg tccgtgaata tacagtcctt tggcacgaac tggtcctctc
     1021 ccacccttcc tacaagtttg ccggtgatct caaacggagc atgaccatct ccaacgtttt
     1081 catttcgatg ctggatatcc acggctacac ggccttttgc cggcaatcca aaaacaacct
     1141 ctcgcagctg catgagctgg acaatttcct caatacgacc atcagggatg tcgccaaaaa
     1201 caacgggtgt atcggcaatc gtgaacgcgg ggacgagatt gtcctggttg gaggtcgggc
     1261 cagtgacatc ctcaaagcca cttttgaaat catccagatc tttgccaggg agaatttctt
     1321 caaacagagc accaatgccg atccctcgga caaatacaac cggatactcc cggcattcaa
     1381 tatctccgcg gggatctctg gcgggaatac caattctccg atgatcatca ctcgtaaagg
     1441 cgaaatctcc ggtttcttgc tcaacacagc ggcccggctc cagaccaggg ccaacaaact
     1501 cgctccgaag catacaaaaa tcatcattgc caagacggtc catacctatc tcgaaaagga
     1561 aaaccggctg caggatttca tgaaatacat cgattttttc gattgtggca gcatcgagtt
     1621 caagggaatg agcatcgcta acatggaggt gctcttccgc gaggccgata aaaaaaagct
     1681 caaataccag aaagtgtttc tcgagatgct ctcctctctc cggcagaacc tctggaaaga
     1741 ccgggtcatg ctggactgcc tctccaccat ggatgctgtc atcactattc ttccgccgat
     1801 gaggatggaa gttgcacttc ccggatacaa gaccctggcc atatcaaacg atcttctgca
     1861 ccgcaagatt actattatcc agaacagctt cagcatccag gatgactaca tcaccgccat
     1921 catcaaattc gacgaattga tggtcattct ccagcaggtt cccgatttcg aaccgatcgt
     1981 tctcgagtat ggacaggaga tcaatgaccg ctacaagatg attctcaccc attatgaacc
     2041 cgagcttgaa cgggagatcg aacagaaaaa aggaatgatc ttttccccgc aggagttgcg
     2101 tgtctacgat tatttcaaga gcaacgccaa ttccctcgaa ctcctggccg agaaggcccg
     2161 caagagtccc cagctttcgc agcgcaagat cctctggaat tcgatgatcg aaaaaaagaa
     2221 gtctgaactg gatttcagcc tgtattccgg gaaaaaataa aggctccatg ccatgcacgt
     2281 acaaaagact gtcctgatct accaggacag ccttttgtac gcgtccgatg ttcagcttcg
     2341 ttctgcgtgt tcgagaagga gtttgctgaa aaactcgcca aatgaagccg gatcctcgat
     2401 tttaccacct tcggcgatga cggcatttgc atagagggcg tccgcaatct caccagccag
     2461 atcggatctg gattcgtcag cctggagctg attgagcttc atgaccaggg catgccctgg
     2521 gttgatctcg agaatccgtt tggccttagg cacatcctgc cgcatggcct tcattagctg
     2581 ctccatgtgg gcgcccatat catattcgtc cgcgacaagg caggacgggc ttgtgacaag
     2641 ccggctcgag acacggactt ccttgatctt gtcctcaaag cgtttgccca tccaggcaac
     2701 gagtcccttc aaatcctcac gctgcttttc ctgtgtcttt ttttcttctt cggttccttc
     2761 gggcagatct cccttggcca gtgacaggaa tttctttccc ttgtattcgg gcatctgagg
     2821 aagaatccac tcgtcaacag ggtccgtgaa gaagagaacc tcgtagctct ttttgcggac
     2881 agcttcaaga tagggagatt tttcgagttc ggcccgggaa tcaccgg
//
LOCUS       DAOSHE010000255         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32295_length_2922_cov_3.689571, whole genome shotgun sequence.
ACCESSION   DAOSHE010000255 DAOSHE010000000
VERSION     DAOSHE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32295_length_2922_cov_3.689571"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1353
                     /locus_tag="PLD82_07305"
     CDS             196..1353
                     /locus_tag="PLD82_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65228.1"
                     /translation="MNAKPLLVSLVLLGISVSSLAADEIRNDLIRFETDIVGNFRLWT
                     TGGDPTVDTDDNQALLNNEAFGSSYVLVSVDGSAARYGSSAGKNLAFSKRVGNEIVSA
                     WEYRGVRFEQKLSLHRSIYSTQSNLMRVEVRVINSDSRQHAVGIRVVLDTIAGNMDQN
                     GFCLPGSGLVQKGTKLETRVPSLLFVCDKQVKGPANLEINLYGQELVTPDLLLVSHER
                     YTKSDTRLVPEIDDSFDDEFTGKSSVSMVWNEKTFPAGANDAVAIALGVSTKKVREGQ
                     PLNMKLALPLSVIGKENVWMGVVLENENKFWDVTKIAATIEYDSTRLALDGTPATAVI
                     DRIDRAGKGFCSWNLRVLREGETKVTVKVSAMYRSMPVRLEYIHTVTVGNR"
     gene            1376..2707
                     /locus_tag="PLD82_07310"
     CDS             1376..2707
                     /locus_tag="PLD82_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65229.1"
                     /translation="MENSFKKRVVRTIVFVSASFAVLALLAFVGINSWLPDYLLKKYI
                     NKSRELAQVLSRSIDGQSIDTPHERLVRRIVQLNFPEERNFVHIVIRDKSGTPMYVER
                     GEHDEQPLSEMLDKKREGVEELSEDGPSQIVQYKERNIVYYTITTPIIQSGKKTGDVV
                     VGFSKKYLTDRIELNRSQVTQLVFLGSISLIVVLLLSFLFIMHLFIKFKKSEETADSF
                     KRMAYVGEISSGLAHEIRNPLNLMSINLQLMREGLEKGETERVLKKIEILEKAKDHAA
                     RILSEFLGFAKMRSKEREIFGMSQIIDEIRNLFSAEVQARQAELEIISTPDDLTIRAD
                     RAEIRQILINLLINALDAVGTREVRTVHIDIQSKRHDLLMTISDSGSGMDEFTLANIY
                     TPFFSKKHGGTGLGLAVVKRIVEEYDGKIECQSIPGQGTTFVVTLKGILAQ"
     gene            2704..>2922
                     /locus_tag="PLD82_07315"
     CDS             2704..>2922
                     /locus_tag="PLD82_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65230.1"
                     /translation="MKILIVDDDPAIAESLAEALSDGNTVCETANDGKAALSLLMSGV
                     WDIAVIDLVMPEKNGIEVLAEARESGIRT"
BASE COUNT          733 a          693 c          812 g          684 t
ORIGIN      
        1 gccgcacgac cctcgacaga ggctgttcac caaaaaaaac aggatgcggg tacgcaaatt
       61 tcctggtaag gtgagcccat ctcacgaaac gacgaaaaca tggcaacgtc gatagcctga
      121 atacgtgatc caacccaatg gcaacatgga tgaatttgtt tgaccttgaa atcaatccgg
      181 tgcatgatgg aggagatgaa cgcgaaacct ctgcttgtca gtctagttct cctcgggatc
      241 tcggtgtcct ctctggctgc cgacgagatc cggaatgatc tgatccggtt tgaaacggat
      301 attgtcggaa attttcggct ctggacaacg ggcggtgacc cgacggtaga taccgatgac
      361 aatcaggcac ttctgaacaa tgaggccttc ggctcgagct atgttcttgt cagtgtcgat
      421 gggtccgcag cccggtatgg ttccagtgcg ggaaaaaatt tggccttctc caaaagggtc
      481 ggtaacgaga tcgtttcggc ctgggaatac cgtggcgttc gatttgaaca gaagctcagc
      541 ttgcaccgaa gtatctactc gacccagtcc aatctgatgc gggtcgaggt tcgtgtcatc
      601 aattccgatt cgaggcagca tgcggtcggg atacgcgttg ttttggacac gattgccggg
      661 aacatggatc agaatggttt ttgcctgcct ggttcaggtt tggtccaaaa gggaacaaaa
      721 ctcgagacga gggttcccag tcttctcttt gtctgcgaca agcaagtcaa aggtccggct
      781 aatctggaga taaatctgta tgggcaggaa ctggtgactc cggatcttct tctggtttcc
      841 catgagcggt ataccaaaag cgatacccgt cttgtgccgg aaatcgatga ttcgttcgat
      901 gatgaattca cgggaaagag ttctgtcagc atggtctgga acgaaaagac ctttcccgcc
      961 ggtgccaatg atgctgttgc catcgctttg ggtgtgtcta ccaagaaggt cagggagggg
     1021 caacccctca atatgaagct tgcgcttccc ctgtctgtta ttggaaaaga gaatgtctgg
     1081 atgggtgtcg tgctcgagaa cgaaaacaag ttttgggatg tcactaagat cgcggcgacc
     1141 atcgagtacg acagtaccag gctggctctg gacgggacac ctgcgacagc ggtgatagac
     1201 aggattgaca gggcaggcaa ggggttttgc tcctggaatt tgcgcgttct cagggaaggc
     1261 gagaccaagg ttaccgtaaa ggtttcagca atgtacaggt cgatgccggt tcgtctggag
     1321 tatattcata ctgtaacagt cgggaacagg taggggacgg gaagcgtccg ctggtatgga
     1381 aaacagtttc aaaaaacgtg ttgtcaggac catcgtcttc gtctcggctt cctttgccgt
     1441 gctggctttg ctggcctttg tcggaatcaa ctcatggctc ccggactacc tgctcaagaa
     1501 atacatcaac aagagccgtg aattggcaca agtcctgagc aggagcattg acgggcagag
     1561 tattgatact ccccatgagc ggctcgtccg gcggatcgtg cagctcaatt ttcccgagga
     1621 acggaatttt gtccatatcg tcatccggga caagagcggg actccgatgt atgtcgagag
     1681 gggtgagcat gatgaacaac ctctctccga aatgctggac aaaaaacggg aaggcgtcga
     1741 ggaactcagt gaagacggac cgtcacagat cgtccagtac aaagaacgga atattgtcta
     1801 ctatacgatt acaacaccga tcattcaaag cgggaagaag accggtgacg ttgtggtcgg
     1861 gttttcaaaa aagtatctga ccgacaggat cgaactcaac cgctcccagg ttacccaact
     1921 ggtttttctc ggatccatct ctttgatcgt tgtccttctt ctgtcgtttt tgttcatcat
     1981 gcatttattc atcaagttca aaaaatcgga agaaactgcg gattccttca agcggatggc
     2041 ctacgttggc gagatttcca gtggcctggc ccatgaaatc aggaatccgt tgaatctgat
     2101 gtcgatcaat ctgcaactga tgcgggaagg ccttgaaaag ggtgagacag agcgggttct
     2161 gaaaaagatc gagattctcg aaaaggcgaa agaccatgcc gcccggattc tcagcgagtt
     2221 tctcggtttt gcgaagatgc gcagtaagga gcgcgaaatc ttcggaatgt cgcagatcat
     2281 cgacgagatc cggaatctgt tcagcgccga ggtccaagcc cgacaggccg aactcgagat
     2341 catcagtacg cccgatgatc tcaccatccg tgccgaccgg gccgagatcc gacagatctt
     2401 gatcaatctc ctcatcaacg ctctcgatgc ggtcggtacg agagaggttc ggacggtcca
     2461 cattgatatc cagtcaaagc ggcatgatct cctgatgacg atttccgatt caggatccgg
     2521 aatggacgag tttaccctcg ccaatatcta cacgccgttt ttttccaaaa agcatggtgg
     2581 aaccggtttg ggacttgccg tagtcaaacg gattgtggaa gaatacgacg gcaagataga
     2641 atgtcagtca attcccgggc aaggtactac ctttgtggta accctgaaag gaattctggc
     2701 ccaatgaaaa tcctgatcgt tgatgatgat ccagccatag cagagtcttt ggcggaagcg
     2761 ttaagcgacg gaaataccgt ctgcgagacg gccaatgacg ggaaagcggc gctctctttg
     2821 ctgatgtccg gtgtctggga cattgccgtc atcgacttgg tcatgccgga gaaaaacggg
     2881 attgaggtcc tcgccgaagc acgtgagtcc ggcatcagaa cg
//
LOCUS       DAOSHE010000256         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32353_length_2918_cov_5.209221, whole genome shotgun sequence.
ACCESSION   DAOSHE010000256 DAOSHE010000000
VERSION     DAOSHE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32353_length_2918_cov_5.209221"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLD82_07320"
     CDS             complement(<1..162)
                     /locus_tag="PLD82_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG65231.1"
                     /translation="MFDPLYLIMTAPVLLLSLWASWRVKSNFEKYSRVRSYSGITGAE
                     AARRILQANG"
     gene            complement(249..917)
                     /locus_tag="PLD82_07325"
     CDS             complement(249..917)
                     /locus_tag="PLD82_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOG65232.1"
                     /translation="MLSKPRYAFFDMDHTITRVDTGLRFLRWWCRRHPLSAWRLLLAP
                     APLLLWKIRILRLRSVKQFLFAAIKGRSVEDVDRAAKEFVRQSLSVVVKPEALVMIAA
                     LAETHTMVLASASPDLYVRHFAAAFGFPYWVSTRYEVCDGRYTGRMLGEDCRDEEKVR
                     RISELVPLDDYDIYESIAFSDNVLADGPMLALAGQQFRVHPKKWQFVPWGGIRPRRAG
                     PAFD"
     gene            1176..2132
                     /locus_tag="PLD82_07330"
     CDS             1176..2132
                     /locus_tag="PLD82_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65233.1"
                     /translation="MKKIAVLLFVLTLALAACGSKTSGDSAAAVGLAGNLIPENASFV
                     GTADVPGLLKIEFIKKMSKDRAEFEKQLGIKTEDLGVMTFWATVSGTKEADAAFFTKG
                     LTGKIIEKLGGSSQEYQGIKVYTIGDKESAVAEVAGYVVFGTLKSVKATIDASKGRNL
                     ASSGRDKEFAAMLSKTQGLVTFAFIPDAKMKREIQAGAKAMKGFEKFIEGFNGLALGI
                     GHASKKLELRIAAGSSDDGAKAAAEMLSGLKEQVGSQIDGFAMMLGKEAAGAAKDALA
                     SFKAKANGSFLNLDLDVPEVLIDAVASQLAGMFGGMAGGLMK"
     gene            2296..>2918
                     /locus_tag="PLD82_07335"
     CDS             2296..>2918
                     /locus_tag="PLD82_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65234.1"
                     /translation="MKRSVHIISLCSILFLFVAAIPAMASVPASLIRAKATIVGTINV
                     PRVLALPVFANLYNDIRQAEAQAGFEFASLGVLPWFAIAHGSQDPAAAAFVRNLPDSV
                     FSRGTQSVHNGTTLYKFTEGDTSVAARIDGWVVLGNLKGVKAVIDAKRSGQTLASSGR
                     TAAFNELAAKSSGLVTISYVPTDRDLRKLKKDFASMSELTPFISGFTG"
BASE COUNT          622 a          799 c          863 g          634 t
ORIGIN      
        1 gccattggcc tgcaggatgc gccgtgccgc ttcggcgccg gtgatgccgc tgtacgaacg
       61 cacgcgcgaa tatttctcga aattgctctt gacccgccat gaggcccaga gcgacaggag
      121 cagcaccggt gcggtcatga tgagatagag tggatcaaac atggtttgtt cctccatata
      181 cgggtatccc gatattggta aaatacccat tttttgtgaa ctgtgcgagt cccgtgatgg
      241 gcggattgtc agtcaaaggc cggcccggcg cggcggggac ggattccgcc ccagggaacg
      301 aattgccatt ttttgggatg gacgcggaac tgctggccgg ccagcgccag catcgggccg
      361 tcggcaagca cgttgtcgct gaaggcaatg gattcgtaga tgtcatagtc atcgagcggg
      421 acaagttcgc tgatccggcg caccttctcc tcgtcccggc agtcttcgcc gagcatccgg
      481 ccggtatatc gtccgtcgca gacctcgtag cgggtggaga cccagtacgg aaaaccgaaa
      541 gcggccgcga aatgtcggac gtacaaatcg ggagaggcgg atgccagcac catggtgtga
      601 gtctcggcca gtgccgcgat catcacaagg gcctcgggtt tgaccaccac agacagggat
      661 tgacggacga attccttggc ggcccgatcc acatcttcga cagaccggcc tttgatggcg
      721 gcgaacagga actgtttgac actccgcagg cgcaggatcc ggatcttcca gagaagaagc
      781 ggggccggtg ccaaaaggag gcgccaggcc gagaggggat ggcgccggca ccaccagcgc
      841 agaaaacgca gaccggtatc cacccgggta atggtatggt ccatatcgaa aaaggcgtag
      901 cggggtttcg acaacatttt ctcgctccgg ctgaagatgc cccaaatata cgcatccgcg
      961 ccggtttctc ccgtcttgcc gaaccgggaa tcccggaatg ggaacggaga atcatgattc
     1021 tgttgtgaga ttccggggaa cccatgttgg ttttgtgata tcacgattat gtgtaccggt
     1081 ccggcttttt accctggatg cttgtctgca caggatttga cggctacttt ttgaaagctt
     1141 tgtacaagcc atcatactct ttggaggaag cacacatgaa gaaaatcgca gtactcttgt
     1201 ttgtcctgac gttggcactt gcggcttgcg gctcgaagac ctcgggtgac agcgctgctg
     1261 cagtcggcct tgccggcaat ctcattcctg aaaatgccag ttttgtcgga acagcggatg
     1321 tccccggtct gctcaagatc gagtttatca aaaagatgtc caaggaccgg gccgagttcg
     1381 agaagcaact cggcatcaag accgaggatc tgggcgtcat gacgttttgg gcaactgtct
     1441 ccggcaccaa ggaagccgat gccgctttct ttaccaaggg actgaccgga aaaatcattg
     1501 agaagctggg tggttcaagc caggagtacc aagggatcaa ggtgtacacc atcggtgaca
     1561 aagaatccgc tgtcgctgaa gtcgccggat atgtcgtttt tggtacactc aagtctgtca
     1621 aggcgaccat cgatgcctcc aagggcagga atctggcttc ctccggacgg gacaaggaat
     1681 ttgcggccat gctttccaag acacagggac tcgtcacctt tgccttcatt cccgatgcga
     1741 agatgaagcg ggaaatccag gctggcgcca aggctatgaa gggctttgag aagttcatcg
     1801 aaggcttcaa cggtctggcc ctcgggatcg gccatgcttc gaaaaagctc gaattgcgca
     1861 ttgcggccgg atcctcggat gacggcgcga aagccgcggc cgagatgctt tccggtctca
     1921 aggaacaggt tggcagccag attgacgggt ttgccatgat gctcggcaaa gaagctgccg
     1981 gggctgccaa agacgcgctt gcttcgttca aagccaaagc caacggatcc ttcctcaatc
     2041 tggacctcga cgttccggaa gtcctgatcg atgccgttgc ctcgcagttg gctggcatgt
     2101 tcggcgggat ggccggcggt ctgatgaagt gatgtgatac ggtcttccgg tcgtgacggg
     2161 gctgtccggt tttcgggcag ccttttttgt tgtgcggaaa tgtgacgatt tctgatcgtc
     2221 cggattcggg gaacacttcg gactgtgcgg tgtccagaag tggtaccggt cagtacaaca
     2281 acaggagaac cacccatgaa acgttctgtg cacattatca gtctctgcag catccttttc
     2341 ctttttgtcg ccgccattcc ggccatggct tccgtcccgg ccagcctgat tcgtgccaag
     2401 gcgaccattg tcggaaccat caatgtcccg cgggttctgg ccctgcccgt atttgcaaat
     2461 ttgtacaatg acatccgcca ggccgaggct caggccggct tcgagtttgc cagcctcggc
     2521 gtcttgcctt ggttcgccat cgcccacggt tcacaggacc cggccgctgc cgcgtttgtc
     2581 cgcaacctgc cggacagcgt tttcagcagg ggaacgcagt cagtccataa cgggacgact
     2641 ctctacaaat tcacggaagg ggacaccagt gttgcggccc gcatcgacgg gtgggttgtt
     2701 ctcggcaatc tcaaaggcgt caaagccgtc attgacgcaa aacgaagcgg acagaccctg
     2761 gcatccagtg gccggacggc tgccttcaat gagcttgctg ccaagagcag tggtcttgtg
     2821 accatttcct atgttccgac agaccgtgac ctgcgcaagc tcaagaaaga ttttgcctcg
     2881 atgagcgagc tgaccccttt tatcagcggc tttacagg
//
LOCUS       DAOSHE010000257         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_32399_length_2916_cov_4.077595, whole genome shotgun sequence.
ACCESSION   DAOSHE010000257 DAOSHE010000000
VERSION     DAOSHE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32399_length_2916_cov_4.077595"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..827
                     /locus_tag="PLD82_07340"
     CDS             342..827
                     /locus_tag="PLD82_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65235.1"
                     /translation="MRFIVLVIVGLAGFLAACSSTGAIRSQWEHRVLASGERTSREYR
                     EEGLLLYRERLVPPAFTSILCPAGSFVYHRPHALVAASSGWLREDANLDSLEVRSGAV
                     SLADKDRQRGWYLAAWDGKRAGTPSDWVWVRIGTNGYWLKPQGLHYYSELLAPPRDGQ
                     R"
     gene            complement(844..1974)
                     /locus_tag="PLD82_07345"
     CDS             complement(844..1974)
                     /locus_tag="PLD82_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG65236.1"
                     /translation="MSGETRESPGRQAATAIIPLIVWLTVDTVWGSLAGLLAGSGVVL
                     LLALHAYLAYRRFDRGLLLDLLLMSAISLPDIPGAIPLPAGLVSPGVELFCAALMLPV
                     FFNRLPRTVTAGLEIPQAIKPLLARVMQAAAILLVLHALLLFGAWASAVEPAGILPSN
                     LPGAIPGTREQWHAVLESASGPLFWILPGLLAAGWIIRTRRRLRTPDPDDASEWLAVV
                     DTRGEEIARARRGLLHTRPDLLQHVVHLVIRDPSNESILLQKRRADRAAAPGLWDTAA
                     AGHVRAGETAQAALVREAREELGITLAGKERWIGAYILRDRQESEYTDLWLLEESGPF
                     RPDRSEMDALVWHTADQIARLADQETITRGALQDIELVNRKA"
     gene            complement(1971..2768)
                     /locus_tag="PLD82_07350"
     CDS             complement(1971..2768)
                     /locus_tag="PLD82_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOG65237.1"
                     /translation="MKSPKEKQIENRILELVTGRALTLAERLFADKEIETLQEYANTV
                     SINRMNFNDHGPVHMKTVARNALVIAGILHRAGIAYTLEREDDGDFEDVQMILLLASL
                     LHDIGMTVGREEHERMGVILSLPILDRILSAIEPDLPKKIAIRSITLECIAGHMASRR
                     IHTLEAGTVLIADGCDMKKGRARIPMILNNEPKPGDIHQYSAAAIEDVSLEAGTRKPL
                     RITVSMTSSVGFFQVEEVLFPKINSSTVKPHVELLAGLPDEEPKCYL"
BASE COUNT          537 a          795 c          964 g          620 t
ORIGIN      
        1 gacggggcgg agaaattcgg aaacaatgtg ttttgatact gcgcccgggc catcctccgc
       61 gccagtggac catctcccga ccacaatttt acccggagga ctaaggctgt tcaccaaaaa
      121 gaacaggatt gggatacggg atgccttcgg gacaggatca tttgcaaacc tgctgacaga
      181 agtaccgggc aggaataaga gacagggcgc gttgttcgac aggaacctgg cagatggccg
      241 gacaggagga gcctccatcc ggaaatcctt gactggatcg ttcttccggt ctatcatttg
      301 aagacagata ttggttcgaa tttgtttttt cgggaaggtg tatgagattt atcgttcttg
      361 tgattgtcgg attggccggg tttctggcgg cctgcagttc gacgggcgcc atccgttcgc
      421 aatgggagca ccgcgttctg gccagtggtg agcgcacatc ccgcgagtat cgcgaggaag
      481 gcctgctcct ctaccgggaa cggctcgtcc ctccagcctt cacttccata ctctgtccgg
      541 ccggatcctt tgtctatcat cgtccccatg ccctggtcgc tgcttcgagt ggctggcttc
      601 gcgaggacgc caacctggat tccctcgagg tccgttccgg tgcagtctcg ctggctgaca
      661 aggatcggca gaggggctgg tacctggcgg catgggacgg gaaacgggcc ggaacaccct
      721 ctgactgggt ctgggtccgg atcgggacaa acgggtattg gctcaaaccg caggggctgc
      781 actattattc cgaacttctc gctccgccca gggatggcca gcgctgaggg ggttgaacca
      841 cgttcatgct ttccggttga cgagttcgat gtcctggagc gcaccccggg tgatggtttc
      901 ctgatctgca agccgggcaa tctggtcggc ggtatgccag accaaggcat ccatctcgct
      961 tctgtccgga cggaaagggc cgctctcctc gagcagccag agatccgtgt actcgctttc
     1021 ctgtctgtcg cgcaggatgt aggcgccaat ccatcgttcc ttgcccgcta gggtgatccc
     1081 gagttcttcc cgtgcttcgc gaaccagggc cgcttgtgcc gtttctcctg cgcggacatg
     1141 gcccgcggct gccgtgtccc agagtccggg ggctgcggcc ctgtcggcgc ggcgtttctg
     1201 gaggaggatg ctctcgttcg agggatcgcg gatcacgaga tggacaacat gttgcaggag
     1261 atccggtctg gtatggagca ggccccgtct ggcgcgtgcg atctcctctc cccgggtgtc
     1321 cacgacggcc agccattcgg aggcatcgtc cgggtcgggg gtgcgtaacc ggcgccgggt
     1381 gcggatgatc cagcccgccg cgagcaggcc cgggagaatc cagaagaggg ggccactggc
     1441 cgattcgagg acggcgtgcc attgctcgcg ggtacccggt atagcgccgg gaaggttgga
     1501 tggcaggatt ccggcaggct cgacagcgct ggcccaggct ccaaacagga gcagggcgtg
     1561 caggacgaga aggatggcgg ctgcctgcat caccctggca agaaggggct tgatcgcttg
     1621 tggaatctcg agacctgccg tgaccgttct ggggagtctg ttgaaaaaga ccgggagcat
     1681 gagagcggca cagaacagtt ccactccggg agaaacgagt ccggccggga gggggatcgc
     1741 acccggaata tcgggcaagc tgatcgcgga catcaggaga agatcaagga ggaggccccg
     1801 gtcgaatctc cggtatgcaa ggtacgcgtg cagggccagg agcaggacga caccgctgcc
     1861 tgccaacaga ccggcaaggc ttccccagac ggtgtcgacc gtgagccaga cgatcagggg
     1921 aatgatggct gtggcggcct gtcttcccgg gctttcccgt gtttcgccgc tcacagatag
     1981 cacttcggtt cttcgtccgg aaggccggcc aagagttcaa catggggttt gaccgtggag
     2041 gagttgattt tcgggaagag gacttcctca acctggaaga acccgacgga gcttgtcatg
     2101 gatacggtga tcctgagggg cttgcgtgtt ccggcttcca gtgaaacatc ctcgatcgct
     2161 gcagccgaat actggtggat gtccccgggc ttgggttcat tattgaggat catcgggatc
     2221 cgtgcgcggc cctttttcat gtcacagccg tcggcgatca ggacggtgcc tgcttcgagg
     2281 gtatggatcc tgcggctggc catgtggccg gcgatgcact cgagggtgat cgagcggatg
     2341 gcgatttttt tggggaggtc gggttcgatg gcgctcagta tccggtcgag gatcggcagc
     2401 gagaggatga cgcccatccg ttcgtgttct tcccggccga cggtcatccc gatatcgtgc
     2461 aagagggacg ccaggaggag gatcatctgg acgtcttcga aatccccgtc atcctcccgt
     2521 tcgagggtgt aggcgatccc ggcacgatgc aggattcctg cgatgaccag ggcgtttcgg
     2581 gccacggtct tcatgtgaac cggtccatgg tcgttgaaat tcatgcgatt gatggaaacc
     2641 gtattggcat actcctgcag ggtttcgatt tccttgtcgg caaagagcct ctccgcgagt
     2701 gtcagtgccc tgcctgttac aagttcgaga atcctgtttt caatctgctt ttctttgggt
     2761 gatttcatcg agcagccccc tcacagggag agtctaggca tgaagtatcc tccggggcaa
     2821 gggtgaggac ggggaatccg gtttgttggc agggagtgac gggttccctg aagtttgaga
     2881 agccagcatc gaggggggcg gttgactatg gctgca
//
LOCUS       DAOSHE010000258         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33025_length_2879_cov_3.184490, whole genome shotgun sequence.
ACCESSION   DAOSHE010000258 DAOSHE010000000
VERSION     DAOSHE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33025_length_2879_cov_3.184490"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLD82_07355"
     CDS             complement(<1..598)
                     /locus_tag="PLD82_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65238.1"
                     /translation="MPQTEAGIRQEVERSLDKNDIITFRTYYLSDYGEMVLRVIASSI
                     LERFGRSDLMDIVYSAAKELIINATKANLKRVVFTELGLDLLSPSEYEKGMHIFKERL
                     VEDKVKEYEKVFKEMDYPVVSTFYYWPNVLNIKVKNSFMLLPEEEKKIRSKFERAHSF
                     ANLLDFYMEYGDDTEGAGLGLAMVGILLDESGIDKHAFT"
     gene            758..>2879
                     /locus_tag="PLD82_07360"
     CDS             758..>2879
                     /locus_tag="PLD82_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG65239.1"
                     /translation="MTLRERLAAGELLLLDGAMGTEIQKKEIRDEDWGGHHGCNEYLN
                     ISAPHLISEVHRLYYAAGSDIVETNTFGASSYVLAEYSLSDQTREINLAAARLARSVA
                     DGFGDGKPRYVAGSIGPGTRLASLGQIEHDAVYAAYLPQIQALADGGVDIFMIETCQD
                     PLHFKTVIEAAQDVIVASGRDILILLSVTIETTGTMLVGSDISAVISVASAMGVDILG
                     INCATGPDLMAPYIREISQTFPGFTMVMPNAGMPQNINGKMVYSLPAQDFAQRVARYI
                     AEDGVQIAGGCCGTSPEYIAALSVATRDIKPAIRQRERAWTLASLYTAQNMQQEPRPL
                     MIGERANTNGSRRFKELLTSENWDGILEVIADQETGGAHALDLCVAYTGRNEVRDMTL
                     AVSKAVTRTRLPLVIDSTSIEVLEASLKLYGGRPLVNSVNLESGEAYAERVCRLAKRF
                     GAALVALTIDEIDGMAKTAEKKLEVARRIHAIAVDRVGMMPQDLVFDVLTFTLGSGDS
                     DSRNAGVETLEGIRRVKATLPGVWTVLGLSNISFGLSPQPRKILNSVFLSEAVDAGLD
                     MAIVHASSIVPMAAIDDGDREAALALIYNKTDNALFDFIKRFEDRSDGSAVTSENQVS
                     LPVEKRLEQAIIRGNRAGLDVMLDEARASHDALSIINEILIPAMKIVGDLFGSGKMQL
                     PFVLQSAETMRAAVEILQPWIEKKD"
BASE COUNT          635 a          758 c          815 g          671 t
ORIGIN      
        1 gcgtgaaggc gtgcttgtcg atcccgctct cgtcgagaag gattccgacc atggccagtc
       61 ccagcccggc gccttcggtg tcatctccgt actccatgta aaaatccagg agattcgcga
      121 acgaatgcgc ccgttcgaat ttggaacgga tctttttttc ttcctccggc aggagcatga
      181 aactgttttt gaccttgatg ttgaggacat tgggccagta gtagaatgtc gacacaacgg
      241 gatagtccat ctccttgaaa accttttcgt actctttgac cttgtcttcg acgagacgtt
      301 ccttgaagat gtgcatcccc ttttcgtact ccgacgggct gaggagatcc agaccgagtt
      361 cggtaaagac gacgcgtttg agattggcct ttgtcgcgtt gatgatcagc tccttggcgg
      421 cgctgtagac aatatccatc aagtcggatc ggccgaatcg ttcgaggatt gaggaggcga
      481 tgacacgcaa caccatttct ccgtagtcgc tcaggtagta ggtccgaaac gtgatgatat
      541 cgtttttatc gagggaacgc tcgacttcct ggcgtatccc tgcttcagtc tgtggcatac
      601 aacatccatt tctgcgggtc ttggtgcccg cgaacagaat cccgcaggtc cacaaacaga
      661 atatgtgatt tggacttcga ttgtcaagag ggatatcgtt tgagacggac cgtgaacgga
      721 gtatattttg tatgcaaacc acaacaggcg ggacaggatg acacttcgcg agcggctggc
      781 agccggagag ctgcttcttc tggatggtgc catgggcaca gagatccaga aaaaggaaat
      841 ccgggatgaa gactggggtg gccatcatgg ctgcaacgag tatctcaata tctccgcacc
      901 acatttgatc agcgaggtgc accggcttta ttatgcggcc ggttccgaca tcgtggagac
      961 gaataccttt ggtgccagca gctacgtcct tgccgaatac agtctctccg atcagaccag
     1021 ggagatcaat ctggcggcgg cccggctggc gcgaagcgta gcggacgggt ttggtgatgg
     1081 caagccgcgc tatgtcgccg gatcgatcgg gccgggaaca cgccttgcca gcctcggaca
     1141 gattgagcat gatgccgtct atgccgccta tctccctcag atccaggccc tcgcggatgg
     1201 tggcgtcgac attttcatga tcgagacctg ccaggacccc ctgcacttca agacagtgat
     1261 tgaagcggcg caggatgtga tcgttgcctc gggacgggat attctgatcc tgctttctgt
     1321 gaccatcgag accacaggaa cgatgctcgt ggggagcgat atctcggccg ttatctctgt
     1381 tgcatccgcc atgggcgtgg acatcctcgg gatcaactgt gcgaccggtc cggatctgat
     1441 ggccccgtat atcagggaga tcagtcagac cttccccgga ttcacgatgg tgatgcctaa
     1501 tgccggaatg ccgcaaaaca ttaacgggaa aatggtctat tcccttcccg cgcaagactt
     1561 tgcccagcgt gtggcgcgct atattgcaga agacggagtc cagattgcgg gcggctgttg
     1621 tgggaccagt cctgaataca ttgctgctct ctctgtcgca acccgagata tcaagccggc
     1681 catccggcaa cgggaacggg cctggacact ggccagcctg tatacggcgc aaaacatgca
     1741 gcaggagcca cgcccgctga tgatcgggga acgtgccaat accaacggga gccgacggtt
     1801 caaggaactc ctcacctccg aaaattggga tggaattctc gaagtgattg ctgaccagga
     1861 gacgggtggc gcgcacgctc ttgatctctg tgttgcctat acggggcgga acgaagtcag
     1921 ggacatgaca ctggccgtct ccaaggcggt cacccgcacc cgacttcccc ttgtgattga
     1981 ttcgaccagt atcgaggttc tcgaggcctc actcaaactc tacggtggcc gccctcttgt
     2041 caactccgtc aatcttgaat ccggagaggc gtatgccgaa cgtgtttgcc ggttggccaa
     2101 acgttttggt gctgcattgg tcgctttgac aatcgatgaa attgatggaa tggccaagac
     2161 agccgaaaaa aaacttgagg ttgcccggag aatccatgcc attgccgtgg atcgcgtcgg
     2221 gatgatgcca caggatcttg tctttgatgt cctgaccttc acgctgggaa gtggtgattc
     2281 tgactcaaga aatgccgggg ttgaaaccct cgaagggata cgaagggtca aagcgactct
     2341 tccgggtgtc tggacggttt tgggtctgtc caatatctcg tttggtctgt caccccagcc
     2401 gagaaaaatc ctcaattccg tttttctgtc cgaagcggtg gatgccggac ttgatatggc
     2461 gatcgtgcat gcttcttcca ttgttccgat ggccgccatc gatgacgggg atcgggaagc
     2521 ggcactggcg ctgatctata ataaaacaga taacgctctt tttgacttca tcaagcggtt
     2581 tgaggatcgc tcggatgggt cagccgtaac ttcagagaat caggtctcgc tcccggttga
     2641 aaagcgactt gaacaggcca tcatccgcgg caaccgggcc ggactcgacg tgatgcttga
     2701 tgaagcgcgt gcgtcgcatg atgccctgtc catcatcaac gagattctta ttcccgcaat
     2761 gaaaatcgtg ggagatctct tcggatcagg aaaaatgcag cttccctttg tgctccagtc
     2821 cgccgagaca atgcgtgctg ctgtcgagat actacagccc tggatcgaaa aaaaggatg
//
LOCUS       DAOSHE010000259         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33046_length_2877_cov_3.962084, whole genome shotgun sequence.
ACCESSION   DAOSHE010000259 DAOSHE010000000
VERSION     DAOSHE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33046_length_2877_cov_3.962084"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLD82_07365"
     CDS             <1..1638
                     /locus_tag="PLD82_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65240.1"
                     /translation="IERVLAVPDDATQNQRHMYDLLWRTLGRLAPGDPRRMQTTLRHE
                     ANWLARVPFQAPEHDASMYWLARIHFEEATRLARRGTGDGQEIHDNFTRGLALQQRAG
                     RYAKDLDPTLVARALLEKKDFMTAIVLLDKLAPEQFPSALGWTRASALMGLGRTNEAA
                     RALEDCLTRPIQIGWHAALYSSWIQTHTALVRINAEKPAVLAGEIPGLDAEVLRAATV
                     LASRERELFDWAQGTHWEYVPWARFVIAWAWSRAAEVALARGDAQEAARWYGRAETLA
                     SRGLGQYEESLNRKTLSHFRRSAEFWHAKLNNVHAETVRYRMLAWVFAGQEDGHPVTP
                     GRQAALARFLTRGFAQLSAFFFYASNGRLLVEYEVRIRPEGIPAYRSGVWQPVDRDPT
                     GKPFAAVNYIEPDLESLGPVKNELFSPANRDRDAFVFLYPGTSSEPSSEKLAGATAGF
                     FHPFGNGTAGGKRARINARFAMANVMATGMIHEMFHHIEDLYRDRSNFVKHVYKREYR
                     HLWPAWYAGTGEIDYYFEVFKRILNPVGFAPMQMREP"
     gene            complement(1643..>2877)
                     /locus_tag="PLD82_07370"
     CDS             complement(1643..>2877)
                     /locus_tag="PLD82_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65241.1"
                     /translation="GTASIRTEGSARSGLTALETLARAFERRPATHQKIDIVFIVETG
                     GSMHRVLNDASETISRMGITLQNGLPVPDVHWGLMTFSGSERLSFHDLSPSSERIAQI
                     IRDEKPGFGDGLLPIDGILQRLVGSVTWRGGSRRYAVLYANGRAPQHGFDAGRLEAPG
                     IRLITIPADDASTGTHQVLQSLARRSGGRSIIPEYLVRYRDQANRDRAFVLKNRTLLR
                     MTHDIPERNWKRYYSGIREPAGGSRRFRAGTIDESIEILGRSGQRGIRIREVQSSLGD
                     AIIAAINSMQTPAGRIRTAHPDALCTALHQGRRISMPVVMDRIHRVTPQAGTWLGINI
                     IPDYYAEGRFRVHPQSLQIFPAHIPPPVLAQQSWSGISRNPGLAVGFALFDPGRIFLN
                     VRDLKIIPIVKKPYILEP"
BASE COUNT          549 a          830 c          903 g          595 t
ORIGIN      
        1 atcgagcgtg tgctcgcggt tcccgatgat gcaacacaaa accagcgaca catgtatgat
       61 ctgctctggc gtaccctggg gcggcttgcg cccggcgatc cgcgccgcat gcagaccacg
      121 ctgcgccatg aggctaactg gctggcccga gttccgtttc aggcccccga acacgatgcc
      181 tccatgtact ggcttgccag gatccatttt gaagaagcga cgcgccttgc gcgaagggga
      241 acaggagacg gacaggagat tcatgacaat ttcacgcgtg ggcttgcgct gcaacagcgt
      301 gccggacggt atgccaaaga cctcgatccg actcttgttg cccgggcctt gcttgaaaaa
      361 aaagacttca tgacggcgat tgtccttctc gacaaacttg cgccggaaca atttccctcg
      421 gccctgggtt ggacacgggc atccgccctc atgggtttgg gcaggaccaa tgaggccgcc
      481 cgtgcgcttg aggactgcct gacccgcccg atccaaatcg ggtggcacgc ggccctctat
      541 tcaagctgga tccagaccca taccgcattg gtccggatca atgccgaaaa accggcagtt
      601 ttggccggag agatcccggg tctggatgca gaagtcctgc gggccgcgac tgttttggct
      661 tcgcgcgaac gggagctgtt tgattgggcc cagggtacac actgggagta tgtaccctgg
      721 gcccgctttg tcatcgcctg ggcctggagc cgggcggccg aggtggcgtt ggcccgcggg
      781 gatgcacagg aggcggcccg ctggtatggt cgggccgaga cgctggcctc ccgcggactc
      841 ggtcagtacg aggagagtct caaccggaag acactgtccc attttcgccg gtcggccgag
      901 ttctggcatg ccaaactgaa caacgtgcat gcagagaccg tccgctatcg catgctggcc
      961 tgggtctttg ccggtcagga ggatggtcat cctgtgacac ccgggcgaca ggcagctctg
     1021 gcccgctttc tcaccagagg ttttgcgcag ttgtcagcct tcttttttta tgccagtaat
     1081 ggacgcttgc ttgtcgaata cgaggtgcgc atccggccag aggggatacc cgcctaccgg
     1141 agcggggtct ggcagccggt ggaccgcgat cccactggca aaccctttgc tgccgtcaac
     1201 tatatcgaac cggatctgga gagtctgggc ccggttaaaa acgaactctt ttcgccagca
     1261 aaccgtgacc gggatgcctt tgtctttttg tatcccggaa cgagcagcga accatcttcg
     1321 gagaaactag ccggagccac ggcgggcttc tttcatccgt tcggaaacgg gactgctggc
     1381 ggaaaacggg ccaggatcaa tgcccgcttt gccatggcca acgtgatggc gaccggtatg
     1441 atccacgaga tgttccatca catcgaggat ctctaccggg acagatcgaa ttttgtcaaa
     1501 catgtctaca agcgcgagta cagacacctc tggccggcct ggtatgccgg aacgggggag
     1561 atcgactatt acttcgaggt gttcaaacgg attctcaatc cggtcgggtt tgcccccatg
     1621 cagatgcggg agccctgagg ttttatggtt cgaggatgta cggttttttc acgatgggga
     1681 tgatcttgag atcccgcacg ttgagaaaga tccggcccgg gtcaaacaga gcgaacccga
     1741 ctgcgaggcc cggatttcgt gatatgccgg accaggactg ttgcgccagg accgggggcg
     1801 gaatatgggc cggaaagatc tgcaggctct ggggatggac cctgaagcgc ccctcggcgt
     1861 agtaatcggg gatgatgttg ataccgagcc aggttccggc ctgcggggtg acacgatgaa
     1921 tccggtccat gaccacaggc atcgaaatcc gccgtccctg atggagggct gtgcagagtg
     1981 cgtccgggtg ggcggtcctg attctgccag ccggagtctg catgctgttg atcgcggcaa
     2041 tgatggcgtc gcccagactg ctttggactt cgcggatccg gattccgcgc tggccgcttc
     2101 gtccgaggat ctcgatcgat tcatcaatgg ttcctgcacg gaagcggcgg ctccctcctg
     2161 ccggttcccg gatcccggag taatagcgtt tccaattccg ttcgggaata tcgtgggtca
     2221 tgcgcaggag ggtgcgattc ttgagaacga aggcccggtc ccggttggcc tgatcccggt
     2281 agcggacaag atactcgggg atgatgctcc ggccacctga ccgtcgggcc aggctctgca
     2341 agacctgatg ggttcctgtg gaggcatcat cggccggtat ggtgatcagg cggatccccg
     2401 gagcctcgag ccttcccgca tcgaagccgt gctgtggcgc gcgaccattg gcatagagca
     2461 cggcgtagcg ccgggatcct ccccgccagg tgaccgagcc aaccagtctc tggagaattc
     2521 cgtcaatcgg gagaaggcca tctccgaacc ccggcttttc gtcccggatg atctgcgcaa
     2581 tccgttcact ggaaggtgac agatcgtgga aggagagcct ttccgagcct gaaaaggtca
     2641 tcaggcccca gtgcacatcc gggaccggaa gcccgttttg cagggtgatc cccattctgg
     2701 agatggtctc cgacgcatca ttcagcacac gatgcatcga tcctccggtt tcgacaatga
     2761 agacgatatc gattttttgg tgcgttgccg gcctgcgttc aaaggctctg gccagggttt
     2821 cgagggcggt cagaccggaa cgggccgagc cttcggtcct gatgcttgcg gttccct
//
LOCUS       DAOSHE010000260         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33095_length_2873_cov_4.379702, whole genome shotgun sequence.
ACCESSION   DAOSHE010000260 DAOSHE010000000
VERSION     DAOSHE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33095_length_2873_cov_4.379702"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..399)
                     /locus_tag="PLD82_07375"
                     /pseudo
     CDS             complement(37..399)
                     /locus_tag="PLD82_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            complement(418..735)
                     /locus_tag="PLD82_07380"
     CDS             complement(418..735)
                     /locus_tag="PLD82_07380"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG65242.1"
                     /translation="MNERDELKILISAITEYSVPKKIVLFGSRAQGRARLDSDFDLCI
                     LYDRIPKRKLEVLQDLYRTVFYLGGHPVDLVVYQECQFEERASRKGTFESVIQAEGEV
                     VYG"
     gene            complement(919..1902)
                     /locus_tag="PLD82_07385"
     CDS             complement(919..1902)
                     /locus_tag="PLD82_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65243.1"
                     /translation="METGTKEPRSPEGCCGVFETRDAHPVVVSDGRECVLPGPIWVGW
                     LGWKGREVCWLPAEKVFPPCPVILPRDQAGFSASDERVSILFMHRLRGQIVSLFGHIA
                     IIVRGRVWNFSAILEENECPRADEFFWRPCLSPFVPEGGTDLPVSLHRAHPIGSSWGI
                     RGGQREAAGSPERFANDTSANKSGPRADRYGRRFMRSMDVLEIAGLGSEAIAALEDAL
                     TQLLGRVNEAAPRSRRGESPYFSPWKMNCAGFVREAFRLAGLAHFHSHLPRDLFVEIG
                     WHFTRQARLPESSVRIERRRMAQVAVPGIRPSSSAVPLLNPASWWRVLRMR"
     gene            complement(1868..>2873)
                     /locus_tag="PLD82_07390"
     CDS             complement(1868..>2873)
                     /locus_tag="PLD82_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65244.1"
                     /translation="FELDQNCNSMSNTYEWCAPGIEAMKERLQKVPCSAFPWWMAQIR
                     EGRDIVISTISDLPPDATAEREMLASQRICSLVVVPLSVREGRCAFLGLDSTKGERNL
                     SEHYVSLLHVAGRIMMNAVLRRDTEEALKESEARARMLLENAADALFLVASDGSIADA
                     NTNACQQTGYARDEIIGLPIQEIDDSFDFDHLRQRMKMAPGAAVFTVEGRIRRRDGSQ
                     YPVETRVCRFPGRGSPMAVAVARDISQRREMEAEQQKLILDLQEALANIKALSGLLPI
                     CSRCKKIRDDKGYWHKVEKYIAEHSTASFTHGLCPDCLEQQYGEDDVHGDRDEGTAES
                     "
BASE COUNT          692 a          885 c          702 g          594 t
ORIGIN      
        1 ccatgccact atgcccgtaa aaaacagaca caaaaatcag ccggttttca aattaacgta
       61 tttcatacaa cgagcaaata tcacatccgt cgaggatatc gccttctcat catccccagg
      121 cagtaattcc atttcgtacg gatatcgcac tgaaacagca aaaagctgaa gctgcgccag
      181 ttcagacatc agatccgaaa catcgcacac catcttgata cctgccacaa gcgccccaaa
      241 gtcatgcgtc cttggaatct caacaccttc aagcgcatac aaccccttta atgccttctc
      301 agcacactgc tgacaatggt aacagatgat ttcgagcgat ctcttttcca tcccaagaag
      361 atatttggca gcatcgaggt catcctttgc gaaatcaagc caagcctcag cgtcttttta
      421 cccatacacc acctctcctt cggcctgaat gacggactca aacgtccctt tacgagatgc
      481 cctttcctca aactggcatt cctgatacac gaccaaatca acaggatgcc ctccaagata
      541 aaagaccgtt cgatagagat cctgcaggac ttccagcttt cgctttggaa tcctgtcata
      601 gagaatgcaa agatcaaaat cactgtccaa cctggcacgt ccctgcgctc gcgaaccaaa
      661 taaaacgatt ttctttggca ccgagtattc agtgatcgct gaaatcagga tcttcaattc
      721 atccctttca ttcatacccg gaccctctgt ctctgccgtc tttcagcatg atttcgcaaa
      781 tggctatatt ccaagtatac aacaggtggc ggattcacgc caccgaaggt gctcatccgg
      841 gcgagggccc cgcctgaggc ggtatacgca taacagcatc acgtttttat catgcgtatc
      901 ccggattgcc cccacccctc agcgcatccg caacacgcgc caccagcttg ccgggttgag
      961 cagcggcacg gcggaagagg agggccggat cccgggcacg gccacctgcg ccatgcgccg
     1021 gcgttcgatc cggacagagc tttcaggcag gcgggcctgg cgggtaaaat gccagccgat
     1081 ttcgacaaag agatcacgcg ggaggtggct atgaaagtgc gccaggcccg ccagacgaaa
     1141 ggcctctcgt acaaagccgg cgcaattcat tttccatggc gaaaagtatg gtgactcgcc
     1201 ccgacgcgag cggggcgcgg cctcgttgac acggccgagt aattgcgtga gcgcatcctc
     1261 aagggcggca atggcttcgg aacccaaccc ggcaatttcc agaacatcca tactgcgcat
     1321 gaaacgccgc ccgtaacggt ctgcccgcgg cccgctcttg ttggctgacg tatcgttggc
     1381 gaagcgttcg ggcgaaccag ccgcttctcg ttggccgccc cgtattcccc atgaagaacc
     1441 gattggatga gcgcgatgta acgagactgg aagatccgtc ccgccctcag gcacaaaggg
     1501 ggaaaggcag ggccgccaga aaaactcatc cgctcgcggg cattcattct cttcaagaat
     1561 cgcagaaaaa ttccagaccc gtccgcgaac aattattgca atatgaccaa agagtgaaac
     1621 gatctgtcca cgaagacggt gcatgaagag gatggagaca cgctcatcgg atgcgctgaa
     1681 tccggcctga tcccggggaa gaatcacggg gcagggtggg aagacttttt cagccgggag
     1741 ccagcaaacc tcgcgtccct tccaaccaag ccaccctacc cagatcgggc ccggaagaac
     1801 acactccctc ccatcggaaa caacaaccgg gtgggcgtcc cttgtctcaa acaccccgca
     1861 gcagccctca ggactccgcg gttccttcgt ccctgtctcc atgcacgtca tcttctccat
     1921 actgttgctc caggcaatcc gggcagagcc catgggtaaa actggccgtc gaatgctcgg
     1981 cgatatattt ctcgaccttg tgccagtagc ctttgtcatc acggattttt ttacaacggg
     2041 aacagatcgg gagcagaccg gagagcgcct tgatattggc cagggcttcc tgcaaatcca
     2101 ggatgagttt ctgttgttct gcttccattt ccctgcgctg actgatatcc cgtgcgacag
     2161 caaccgccat gggtgaaccc ctccccggga aacgacagac ccgagtctca accggatact
     2221 gacttccgtc tcgccggcga atccggcctt caactgtaaa gactgcggcc cccggcgcca
     2281 tcttcatccg ttgccggaga tgatcaaaat caaaactgtc atcgatttcc tgaataggca
     2341 gtccaatgat ttcgtcccgg gcatagccgg tctgctggca ggcattggta ttggcatccg
     2401 ctatacttcc gtcagaagca acaaggaaga gggcatccgc agcattctcc aaaagcatcc
     2461 tggcccgggc ctccgattcc ttcaaggcct cctcggtgtc ccgcctgaga accgcgttca
     2521 tcataatccg cccggcaaca tgcaggagcg aaacatagtg ctcactcagg tttcgttcac
     2581 cctttgttga gtccaacccg aggaaggcac agcgcccttc tctgacggag agcgggacga
     2641 ccaccaggga acagatccgc tgggaggcaa gcatctccct ctcggcggtt gcatcgggag
     2701 gaagatcgct gatcgtactg atgacgatat cccggccctc tcgtatctgc gccatccacc
     2761 aggggaaggc actacaggga accttctgca aacgctcctt catggcttca atcccggggg
     2821 cacaccactc gtaggtattg ctcatgctgt tgcagttctg atcgagctca aag
//
LOCUS       DAOSHE010000261         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33415_length_2854_cov_4.247588, whole genome shotgun sequence.
ACCESSION   DAOSHE010000261 DAOSHE010000000
VERSION     DAOSHE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33415_length_2854_cov_4.247588"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLD82_07395"
     CDS             <1..549
                     /locus_tag="PLD82_07395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOG65245.1"
                     /translation="NSIFANGGTMTGSIGVIMSSPDMHGLYEWARINWNVIKSGRFKD
                     ILSPWRGMTDEERQLLSDTVLDAFSQFFEVVHTARTIEKAKLEGLAQGQVFSGNQARE
                     QGLVDEVGDFEAALLAAGKLAGLKGRPHVIRQKEQAGIMELLGLAVRNLATPRTITIA
                     GLGVTGGAGTPVIAYLMPGVGR"
     gene            546..1145
                     /locus_tag="PLD82_07400"
     CDS             546..1145
                     /locus_tag="PLD82_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65246.1"
                     /translation="MKAAGILAGSIMAPARTADLVAARREPGLAAPAFFAMAGGVVSA
                     LLGMLVVQGTDGATGRSLFSTLGILLPVTGLFVLLCKVAMIHVFAGLWGQRGDVRALW
                     TGISISWAPFLLLLPASLLLRAAGLEGFFALTVLAAMGMGWYLEVRVIRAVYRLETGR
                     AVALFVLPVVLTVVLVLLVLLALSALVSSLAVAAFGLVL"
     gene            1159..2580
                     /locus_tag="PLD82_07405"
     CDS             1159..2580
                     /locus_tag="PLD82_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG65247.1"
                     /translation="MKILTRYMLRSLIPNYFTGLAFFTVILLVNEVFRMVKYVVERNI
                     PFGRVSQLFIFTVPYVLALTIPMAVVIASILTFGRMSSESEVVALRGSGISLMRILWP
                     NLFFGIILFGVAMLFYDTVLPWGNTRYTEMRSSIFIRDPMADFEAGQVVRIGNQTLRY
                     EREDEGTKLMHNVYITSPDGGVVFAKRGEFIEKRLLKDKMFISFRLHDVTIDENDKKE
                     PEQLLRTHAPTVIQTFVEPFQQVQEVARNARTMSISELWAKMQRDDGARIKLLDEAKK
                     RLGVLQKDLTGSRRRLEEIYNQDPSIPRAHREALQNTSAQAQVSKKGAIVPDTQDRKT
                     APGSVGSPALPGEQANLERRVSELGAQARNQEKRVRELETSKNYVLADDVYEFHKKFA
                     IPFACFVFTIVGAPLGMFSRRSGRGMGFGWAIIVLIVYYVLLTLGQGWAVSNKLAPAL
                     SAWLPDIFLGIIGAFLVLKKLRE"
     gene            2583..>2854
                     /locus_tag="PLD82_07410"
     CDS             2583..>2854
                     /locus_tag="PLD82_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65248.1"
                     /translation="MSGALKALLSPIMRWPGRVWRAFGAVIDLFFRRLLLPVLRLAGR
                     ILKAILKLLHLDTIFRPVVRVVVRGAHGLVAGWRWVGAHIKYINPF"
BASE COUNT          500 a          811 c          875 g          668 t
ORIGIN      
        1 aacagcatct tcgcgaatgg cggaacgatg accggatcga tcggggtgat catgtcctct
       61 cccgacatgc atggcctgta tgagtgggcc cgcatcaact ggaatgtgat caagagcggg
      121 cgtttcaagg acatcctctc accctggcgc gggatgacgg atgaggaacg ccagcttctt
      181 tcggatactg tcctggacgc cttctcccag tttttcgagg tggtgcatac ggcccggacg
      241 atcgagaaag caaaacttga gggcttggcc cagggccagg tgttttccgg gaaccaggcc
      301 cgggagcagg gtctggtaga cgaggtcgga gatttcgaag ccgccctctt ggcagcgggg
      361 aaactggctg gtctcaaggg ccgcccgcat gtcatccgtc aaaaggagca ggccgggatc
      421 atggaactcc tgggactggc tgtgcgcaat ctggcaaccc cccgaacgat caccatcgcc
      481 ggacttggcg tgacaggcgg tgcggggact cccgtgatcg cctacctcat gcccggggtc
      541 ggccgatgaa ggcggccgga atcctcgccg ggagtatcat ggcccctgcc aggacagcgg
      601 atctggttgc ggccagacgc gaacccgggc tggccgcccc ggcgtttttc gccatggctg
      661 gcggcgtcgt ctcggcactc ctcggaatgc tggtggtcca ggggacggac ggggcgaccg
      721 ggcgatccct cttttcgacg ttggggattc ttcttcctgt cacggggctc ttcgtccttc
      781 tctgcaaggt ggcgatgatc catgtttttg ccggactctg ggggcagcgt ggagatgttc
      841 gcgcactttg gaccgggatc tccatctcct gggctccctt tttgctcctt ctgccggcct
      901 ccctgctcct gcgtgccgcg ggtcttgaag ggttttttgc cctgaccgtt ctggccgcca
      961 tgggcatggg ttggtatctc gaagtccggg tcatccgggc cgtgtaccgt ctggagaccg
     1021 gtcgggctgt tgcactcttc gtcctgcctg tcgtcctgac cgtcgtgctt gttcttctgg
     1081 ttcttctcgc cctctccgcc ctggtgtctt ccctcgcggt tgccgccttc gggctggtcc
     1141 tctagagggc ggccggagat gaagatcctc acccgctaca tgctgcgttc actcatcccg
     1201 aattatttta ccgggctggc gttttttacc gtgatcctcc tcgtgaacga ggtctttcgg
     1261 atggtgaaat acgttgtcga gaggaatatt cccttcggcc gggtttcaca gctcttcatc
     1321 ttcaccgttc cctacgtgct cgccttgacc attcccatgg cggttgtgat cgcttccatc
     1381 ctgacattcg gaaggatgtc gagcgagagc gaggtggtgg ccctgcgcgg gagcggaatc
     1441 tccctgatgc gaattctttg gcccaatctc tttttcggga tcatcctttt cggggtggcc
     1501 atgctgtttt acgataccgt attgccctgg ggaaacaccc ggtatacgga gatgcggagt
     1561 tcgattttta tccgtgatcc gatggccgat ttcgaggcgg gtcaggttgt ccggatcggg
     1621 aaccagacac tgcgctacga gcgtgaggac gaaggcacca agctgatgca caatgtctac
     1681 atcaccagcc ccgatggcgg cgtggtgttt gccaagcgtg gcgaattcat cgaaaaacgc
     1741 ctccttaagg acaaaatgtt catcagtttc cgtctgcatg atgtgacgat tgacgagaac
     1801 gacaagaagg agcctgagca attgctgcgg acacacgcgc ccacggtgat ccagactttt
     1861 gtcgagccat tccagcaggt tcaggaggtt gcccggaatg cccggaccat gagtatcagt
     1921 gagctctggg ccaagatgca gcgggatgac ggtgccagga tcaagcttct cgatgaggcc
     1981 aaaaaaaggc tgggggttct ccagaaggat ctgaccgggt ccagacgccg gctcgaggag
     2041 atctacaacc aggatccatc cataccgcgg gcacatcggg aagctctcca gaacacttcc
     2101 gcccaggcgc aggtctccaa aaagggggcc atcgtaccgg atacacagga caggaagacg
     2161 gcccccggct ctgtcggttc tcccgctctt ccgggggagc aggccaatct tgaacgtcgg
     2221 gtgagcgagc tgggtgccca ggccaggaat caggagaaac gggttcgtga actcgagaca
     2281 agcaaaaact atgttctggc cgatgatgtg tatgaattcc acaagaaatt tgccataccg
     2341 ttcgcctgct tcgtcttcac gattgtcggt gccccgctcg ggatgttctc gcggagatcg
     2401 ggcaggggaa tggggtttgg ctgggccatc attgtcctga tcgtctatta tgtccttctg
     2461 acgctcggac aggggtgggc agtctccaat aaactggcac cggcactctc ggcatggctt
     2521 ccggacatct ttttggggat catcggggcc tttctcgtgc tgaagaaatt gagggagtga
     2581 cgatgtccgg agcactgaaa gcgttgcttt ccccgatcat gcgctggcca ggtcgtgtct
     2641 ggcgtgcctt tggtgcggtg attgatcttt ttttcaggag gctgctcctc ccggtcctgc
     2701 gcctggctgg ccgtatcctg aaggccatcc tcaagctcct ccatctcgat acaatttttc
     2761 gtcccgttgt gcgcgtggtt gtccgtggtg cccatggcct ggttgccggc tggcgctggg
     2821 tcggagccca tatcaagtac atcaatccct tctg
//
LOCUS       DAOSHE010000262         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33532_length_2847_cov_3.996777, whole genome shotgun sequence.
ACCESSION   DAOSHE010000262 DAOSHE010000000
VERSION     DAOSHE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33532_length_2847_cov_3.996777"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1365)
                     /locus_tag="PLD82_07415"
     CDS             complement(103..1365)
                     /locus_tag="PLD82_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOG65249.1"
                     /translation="MELVAPAGNAERMEMAFAWGADAVYAGADRYSLRARAENFSDRE
                     LQDSIARAHLLGKPFYLAVNAFFDDDLLTGLAEFLTGLGEPGPDALIMSDPGAMAVAS
                     RVRPDLPIHVSTQANTMNAETVRVLRDRFGIKRVVLARELTLDQISRICHALPDIEFE
                     VFVHGAMCVAYSGRCIVSSYLTSRAFLAPGEKMPGAIDRIRSASLGDCSQTCRWSYGL
                     VEEKREGVVLDIVEDDGKTMLLSSRDLCMVDHLGALRDAGVHALKIEGRMKSLQYAAV
                     TSGIYRDALNHLGSGQEVTPERRGLWHDELDALSRREYSTGFYFLDNEAFVPSQCDRQ
                     PPWLMAGVVLGAVGQGVWRCQATNPVREDAVLSAFMPDLEKSRLIGFRLFGPDGDARS
                     LVRPGEEFLLRTRARLGPMSILRLDSGR"
     gene            complement(1365..2831)
                     /gene="gltX"
                     /locus_tag="PLD82_07420"
     CDS             complement(1365..2831)
                     /gene="gltX"
                     /locus_tag="PLD82_07420"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG65250.1"
                     /translation="MSQIRVRFAPSPTGYLHIGGLRTALFNYLFARKTGGSFILRLED
                     TDRTRLVPDALDDIMRCMRWLGIDWDEGPDKGGAYGPYTQSERLALYQKHAQDLLDSG
                     HAYRCFCTPERLEAMREAQKSDTRYDGHCRSLPQAEVQRLMDAGLPSVVRLKVPTAGE
                     TVVHDFLRGEMRFDNRLQDDLVLLKTDGFPTYHLANIVDDHHMKISHVLRGEEWISSA
                     PKHVMLYDAFGWTPPVFVHLPVILSPNGGKLSKRDGATNVREFIEIGYLPEAMTNFLA
                     LLGWSYDGERSMFTMEELEKEFVLEKIGMASPVFDRLKLDDYNGKYIRLCDDAKLADL
                     CRPRLEAAGFIVPGDPEDERILGEIMPLFRERLVLLSDIVDQAAYFFTDDLDWGDGSL
                     LVPKKTEPGDVLRILDLAHKRLSLLDEYTHAGLEAALRSLVEELAMKAGQVFMPLRVA
                     VTSRAVSPGLFETMAVLGKERCLERIVRAREVLAAHRL"
BASE COUNT          664 a          877 c          783 g          523 t
ORIGIN      
        1 gttgacaata ccgcaaaaaa tgcaatcctt cacgcagact ccctcgacag cagtatgaat
       61 aaaaatactg gaagaatgga gaaaaaagca aggattttgc ctttatctcc cggaatcaag
      121 ccgtaaaatc gacatcgggc ccaaccgggc tcgggtccga agaaggaact cctccccggg
      181 acggaccagg cttctcgcat ccccgtcagg accaaaaagc cggaatccga tcagccggga
      241 tttctccaga tcgggcatga aagccgaaag gacagcgtct tccctgaccg gattggtggc
      301 ctggcaacgc cagactccct gacccaccgc acccaacacc actccggcca tcagccaggg
      361 aggctgccgg tcgcactggc tcggaacaaa ggcctcgtta tccagaaaat aaaaccctgt
      421 cgaatactcg cgccggctga gcgcatccag ttcgtcatgc cacagcccgc gcctttccgg
      481 agtgacctcc tgcccgctcc cgagatgatt caaggcgtcc cggtagatgc cgctggtcac
      541 ggcggcatac tggagggatt tcatccggcc ctcgattttg agcgcgtgca cgccggcatc
      601 ccgcaacgct ccgagatgat cgaccatgca gaggtccctg ctcgagagaa gcatggtctt
      661 gccgtcatct tccacaatgt ccagcacgac gccctcccgc ttttcctcga caaggccata
      721 ggaccagcgg caggtctgac tgcaatcccc gagactggca ctccggattc tgtcgatcgc
      781 tccgggcatt ttctcgcccg gggcaaggaa ggccctgctg gtcagataac tggagacgat
      841 gcaccgccct gaatacgcca cgcacatcgc cccatggaca aagacctcga actcgatgtc
      901 cgggagggcg tggcagattc gcgaaatctg gtcaagcgtc agttcccggg caagcacgac
      961 ccgtttgatc ccgaaccggt cccgcaggac acgaaccgtc tcggcattca ttgtattggc
     1021 ctgtgtcgag acatggatcg gaagatcggg gcggacacga gaagcgacag ccatggcccc
     1081 cggatcactc atgatcaagg catcaggacc gggttctccc agtcccgtca gaaactctgc
     1141 caaaccggtc agaagatcgt catcaaaaaa cgcattgaca gcgagataga agggttttcc
     1201 cagcaggtgg gccctcgcta tcgaatcctg caactcccgg tcgctgaagt tctcggcccg
     1261 ggcccggagc gagtagcggt cggctccggc ataaaccgca tccgcacccc aggcaaaggc
     1321 catctccatc cgttcggcat tcccggccgg agcgacgagt tccatcacaa gcggtgtgcg
     1381 gccagaactt cccgggcccg tacaatcctc tccagacaac gttccttccc gagaacagcc
     1441 attgtttcaa agagtccggg agaaacggcc cgggaggtga ccgcaacccg caagggcatg
     1501 aagacctgac cggctttcat ggccagttct tccaccaggc tccgcaaggc cgcctccaga
     1561 ccggcatgag tgtattcatc aagaagactc agcctcttgt gcgccagatc caggatacgg
     1621 agcacatccc cgggttcggt ctttttggga accaggagac tcccgtcgcc ccagtccagg
     1681 tcatccgtaa agaagtacgc ggcctgatcg acgatatcag aaagcaggac caaccgttcg
     1741 cggaagaggg gcatgatttc tccgagaatc cgctcgtcct caggatcacc ggggacgatg
     1801 aaaccggccg cctcgagccg gggacggcag agatcggcca gtttcgcgtc atcacagaga
     1861 cggatatact tgccattgta gtcatccagc ttgagccggt caaaaaccgg actggccatg
     1921 ccgatttttt caagaacaaa ttctttttcg agttcttcca tggtaaacat ggaccgctca
     1981 ccatcatagg accatccgag cagggccaga aaattggtca tcgcctcggg gagatacccg
     2041 atttcgatga attcacggac attggtcgcc ccgtcacgtt tggaaagctt ccctccattt
     2101 ggagaaagaa tcaccggaag atggacaaag acgggcggag tccagccgaa ggcgtcatag
     2161 agcatgacat gcttgggagc actggagatc cactcctccc cccgcaagac atgggagatc
     2221 ttcatgtggt gatcatcgac aatattggcc aggtgatagg tcggaaagcc gtccgtcttg
     2281 aggagaacga gatcatcctg gagacgattg tcaaaccgca tctctccccg caaaaaatcg
     2341 tggaccacgg tctcgcctgc agtggggact ttgagacgga cgacactggg cagaccggca
     2401 tccatcaggc gctgcacctc ggcctgcggc agggaccggc aatgaccgtc atagcgcgta
     2461 tcgctcttct gcgcctcccg catcgcttcc agccgctccg gggtacaaaa acagcgatag
     2521 gcatggccgg agtccagcaa gtcctgggca tgtttctggt acagtgcgag gcgctcactc
     2581 tgcgtgtagg gtccataggc cccacccttg tccggacctt cgtcccagtc aatccccagc
     2641 cagcgcatgc agcgcatgat gtcgtccagc gcatcgggaa ccagtctggt ccggtcggta
     2701 tcttcgaggc ggaggataaa tgaccccccg gttttacggg caaaaagata gttgaagagt
     2761 gcagtccgca gcccaccgat atggagatac ccggtcggac tgggggcaaa acgaacgcga
     2821 atctggctca tgtacggtat ccttgga
//
LOCUS       DAOSHE010000263         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33780_length_2834_cov_3.529687, whole genome shotgun sequence.
ACCESSION   DAOSHE010000263 DAOSHE010000000
VERSION     DAOSHE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33780_length_2834_cov_3.529687"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2636)
                     /locus_tag="PLD82_07425"
     CDS             complement(<1..2636)
                     /locus_tag="PLD82_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG65251.1"
                     /translation="MKLEHGFEILRTLKIPELGSEARIYRHVKSGAELVSISNNDDNK
                     VFGISFGTPPRDSTGVAHILEHSVLCGSKKYPLKEPFVELIKGSLNTFLNAFTYPDKT
                     CYPVASQNTQDLYNLADVYMDAVFHPLIDEWTFMQEGWHYETTDRKSPLTRKGVVYNE
                     MKGVYSSPDSLLHEFSQRILFPDSIYGNDSGGDPRVIPSLDYASFSAFRERYYHPSNA
                     RIWFWGDDDPGTRLVWLDERLSEFDAKPCEAVIKPQPALTAPRTEEMFYAASPEEPNP
                     KAMFTLNWLLPEKHDASIALAFHILEEILIGQAASPVKRALIESGLGEALAGIGLESE
                     LRQMYFSVGLRGIGTADIPAAEAIIFETLEALAAGGVPQEIVQAALNTVEFALRERNT
                     GRFPQGLSMMLTALTTWLYGRDPLMLLPFERPLEEIKSRIASREPVFETLIREHFINN
                     PHRVGLRLLPDTGLAERQAHEEATELAEIRAGLVPAEVDRIIATTLALEERQQREDDP
                     AALAGLPSLGVQDLDRETKKLPRGTSDRPDLLINIPETSGIVYVDLAFDCAGMPAGLL
                     PWARTWAHALTEIGTESEDYAAFATRIASETGGIRTSDIIAPTVSGGTTSSLVVRARV
                     LAQNVDKLAAILEAMLLTPRLGDVERLARIVLSRRARIEESLVSSGHLIVDSRVRAML
                     HPSGAISEAIRGLGEFAALSAAESTPDNTLLKEIHANLAVRERVTLSITASPGLAESA
                     AKTVTSVLDRLPAQGSWPREGGLPATGAAALALTGPLKVHYVGRGLNMAAAGYKPHGA
                     AILACRAISTGWLWNSVRVQGGAYGAFCRYSPYSGILTMASYRDPNLENTLKRYGETA
                     DWLREQAFTGRESERAIIGAIG"
BASE COUNT          510 a          794 c          940 g          590 t
ORIGIN      
        1 tcgccgatgg ccccgatgat ggcgcgttcg ctctcccggc cggtgaaggc ctgctcgcgc
       61 agccagtcgg cggtttcccc gtaccgtttg agcgtgtttt ccagattcgg gtcgcggtag
      121 gaggccatgg tcaggattcc cgagtacggt gagtaccggc agaaggcccc gtaggcgccg
      181 ccttgtacac ggacactgtt ccagagccag cccgtcgaga tggcccggca cgcaaggatc
      241 gctgcgccat gcggtttgta tcctgcggcg gccatgttca gaccccgtcc gacatagtgc
      301 accttgaggg ggccggtcaa ggccagagcg gctgcaccgg tggctggaag gccgccctcg
      361 cggggccagc ttccctgtgc cggaagcctg tcgagaacgg acgtgaccgt ttttgccgcg
      421 ctctccgcca gtcccggtga agccgtgatg gagagggtca ctcgctcccg gacggccagg
      481 ttggcgtgaa tctctttgag aagcgtgttg tccggggtgc tttccgcagc ggagagcgcc
      541 gcgaactcgc cgagcccacg gatcgcttcg ctgattgcgc ctgacgggtg gagcatggcg
      601 cgcacccggc tgtcgacaat aagatggccg gacgagacga gggattcctc gatccgcgcc
      661 cgccgggaga gcacgatccg ggcgaggcgt tcgacatccc cgagtctcgg agtgaggagc
      721 atcgcttcga ggatggccgc cagcttgtcg acgttctgcg caaggacccg tgcgcggacg
      781 accaaggatg aggttgttcc tcctgaaact gtcggtgcaa taatgtccga tgtccgtatt
      841 ccgcctgttt cggaagcgat ccgggtggca aaggcggcgt agtcctcgga ttccgttccg
      901 atctccgtca aggcatgggc ccaggtccgt gcccagggga gcagcccggc cggcatgccc
      961 gcgcagtcaa aggccagatc gacatagacg atgcccgacg tctcgggaat gttgatgagg
     1021 agatccggtc tgtcgcttgt cccccggggg agctttttgg tctcgcggtc cagatcctgt
     1081 actccgagtg acggcaggcc ggcaagagcg gccgggtcat cttcacgctg ttgacgctct
     1141 tcgagggcca gggtggtcgc gatgatccgg tccacctcgg ccgggaccag gccggcacgg
     1201 atctctgcca gctcggtggc ttcctcgtga gcctgccgtt cggcaagacc ggtatcaggg
     1261 aggaggcgca agccgacgcg atgcggattg ttgatgaagt gttcccggat gagggtttca
     1321 aagacgggtt ctctggatgc gatccgcgac ttgatttctt ccagtgggcg ctcgaaggga
     1381 aggagcatca gcgggtcccg gccatagagc caggttgtca gggctgtcag catcatcgag
     1441 agtccctggg gaaagcgccc ggtgttgcgt tccctgaggg cgaactcgac ggtattgagt
     1501 gccgcctgga cgatctcctg cgggacaccg cctgcagcga gtgcttcgag ggtttcgaag
     1561 atgatcgcct cggccgcggg aatgtcggcc gtaccaattc cgcgcaggcc aaccgaaaaa
     1621 tacatctggc ggagttcgct ctccaggccg atcccggcca gggcctcacc gagtccgctc
     1681 tcgatcagtg cgcgcttgac cggcgacgcg gcctgtccga tcaggatctc ttcgaggata
     1741 tggaaggcga gggcaatcga ggcatcatgt ttttcgggga ggagccagtt cagggtgaac
     1801 atcgccttcg ggttgggttc ctccggggat gcggcataaa acatctcctc ggtccgcggg
     1861 gccgtcaagg cgggttgcgg cttgatgaca gcttcgcagg gcttggcgtc aaattcggag
     1921 agtcgctcat ccagccagac gagccgggtc ccgggatcgt catctcccca aaaccagatc
     1981 cgggcgttgg agggatggta gtagcgttcc ctgaaggcgc tgaaggatgc gtaatccagg
     2041 gaggggatca cgcgcggatc gccgccggaa tcatttccgt agatcgaatc cgggaagagg
     2101 atgcgctgcg agaattcgtg aagcagactg tcgggcgagg aatagacacc cttcatttcg
     2161 ttgtagacta cccccttgcg cgtcagcgga ctcttgcggt cggttgtctc gtaatgccag
     2221 ccttcctgca tgaaggtcca ctcgtcgatc aggggatgaa agacggcatc catgtagaca
     2281 tcggccaggt tgtagaggtc ctgcgtgttc tggctggcta ccgggtagca ggtcttgtcg
     2341 gggtaggtaa aggcgttcag gaaggtattc agtgaaccct tgatcagttc cacaaagggc
     2401 tctttgagcg gatatttttt cgagccgcag aggacggagt gttcgaggat gtgggccacg
     2461 ccggtcgagt cgcggggcgg ggtcccgaag ctgatcccga agaccttgtt gtcgtcattg
     2521 ttggagatgg acaccagctc ggctccgctc ttgacgtggc ggtagatccg tgcctcggat
     2581 cccaattctg gaatcttcaa ggttctcaat atttcaaatc cgtgttcgag cttcatgagt
     2641 ttgcgttacc cgtcctaatt ctgtttttgg ggaacagtcc tccggccccg gcgggcttcg
     2701 gtccttcccc ggatgcattg tacctgctcc ggctccgtgc aatccagtaa aaaagtattt
     2761 caatcgggat gggggaagtg aatggaaagt tatatgctca aatagatata tttgcggagc
     2821 gaatatgcag aatt
//
LOCUS       DAOSHE010000264         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33919_length_2825_cov_3.658484, whole genome shotgun sequence.
ACCESSION   DAOSHE010000264 DAOSHE010000000
VERSION     DAOSHE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33919_length_2825_cov_3.658484"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PLD82_07430"
     CDS             <1..96
                     /locus_tag="PLD82_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOG65252.1"
                     /translation="DNAVTVRERDSMTQTRVPVDSLVSWLAERLD"
     gene            316..1056
                     /locus_tag="PLD82_07435"
     CDS             316..1056
                     /locus_tag="PLD82_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65253.1"
                     /translation="MLRSSVFLVLLFVLASCNAFSWAASVDDSEDYGLLMAEARAQMA
                     AGCPDKAVEYFTRALSLRTNDILALDGRVEARLLLLTSGRSVYTAFPSLFITNGSSDT
                     SLFPHTPTRLADIFPTALSAVRDVDLLDTLLSPTNAIRTARLTADAAVATGLAALLTL
                     ADSNSNGIPCESADTFLPTAAFTVSQSGPAPNPGIVATAQRYALTARVWLDRLESATL
                     PAMRPGTPLSAFRFNLVRFVGHLSGVTP"
     gene            1053..2222
                     /locus_tag="PLD82_07440"
     CDS             1053..2222
                     /locus_tag="PLD82_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65254.1"
                     /translation="MRVPRQMRTTMVLAFLACAFPMTLTARERPFSPGSSRAQAMGMT
                     WIACTASEDAAFLNPAWLATEDERFTIIRLRTAANTDGTDLLFGQTGDDLMTFGSSST
                     RGLPPAGFMDTMTGFDAVYDTSGPLFLSYKGEGFGIGLYSSSRGSFAVHGTTLPTVNL
                     EHDFDIGTIAGLAIPITLNEDTGLQLLIGGTIKYLIRIRHISSDVPLTEASRLVDPFA
                     FSGDFRIGQAIGSDLGITWRSPYFSVGIVWYDWFGTTLSWTEYSGEFKTKGTSLVDTH
                     ITPMIGAGVAWTPPDFLGLPSWVFSNTQIAIDVRGFLEEEDSFFKMLHVGFESTFLHF
                     ATIRLGLNAGYPTCGLAFLLFGSLHMEYTIATEERGRLPGQDPVTLHTLSFTFML"
     gene            complement(2238..2573)
                     /locus_tag="PLD82_07445"
     CDS             complement(2238..2573)
                     /locus_tag="PLD82_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65255.1"
                     /translation="MNDGWKCLEEAPRFEIDRYTRVKVCNETHVAFIGVPNKHRDSEE
                     RMVLACGVDSGAPTFLDFALRDVDRMEQVETMVTVDGKAVPLMRVWVRKGALAFRYLP
                     FIVEEVLLP"
BASE COUNT          539 a          981 c          677 g          628 t
ORIGIN      
        1 gacaacgccg tcacagtccg ggaacgcgac agcatgaccc agacccgcgt ccctgtcgac
       61 agcctggtct cctggcttgc ggagcgtctt gactgaaccg aaggcaatcg ccaatcagga
      121 acgcccttcc ggtttcggcc gggggcgttc ctttttttct gctgcacctt ccacgacacg
      181 ccgtccatca ctgatccgtg gttgttcccc ataaaaaaac cggaacaaaa accgtttttc
      241 cccgtttgat accatgcatt cggcgaattg cgcgctatat ttgatacaaa cttcctttcg
      301 aggagaggac accgaatgct tcgctcatcc gtctttcttg tcctcctctt cgttcttgca
      361 tcctgtaacg cgttttcctg ggctgcctcg gttgacgaca gcgaggatta cggacttttg
      421 atggctgagg ccagagccca aatggccgcc ggctgtccgg acaaagccgt cgagtatttc
      481 acacgtgccc tctccctgcg caccaacgac attctggccc ttgacggaag ggtggaggcc
      541 cggctcctcc tcctgacctc gggtcgctcc gtctatacgg cctttccatc cctcttcatc
      601 accaatggat cttccgacac atcgctgttc ccccacacgc ccacccgatt ggcagacatc
      661 ttcccgacgg cactctcggc cgtccgggat gttgacctcc tggacacact cctctccccg
      721 acaaacgcca tccgaacagc ccgtctgacc gccgatgcgg ccgtggcaac gggcctggca
      781 gcactcctga ccctggccga cagcaatagc aacgggatcc cctgcgaatc agccgacaca
      841 ttcctcccga cagcggcctt taccgtttca caatccggcc cggcacccaa cccgggcatc
      901 gtggccacag cacaacgata cgcattgacc gcaagggtct ggcttgaccg gcttgagtcg
      961 gcaaccctgc ccgccatgcg tccgggtact ccgctctctg ccttcaggtt caacctggtc
     1021 cgcttcgtcg gtcatctgtc aggagtcact ccatgagagt cccccgacag atgcgcacca
     1081 ccatggttct tgcattcctc gcctgtgcct tcccgatgac actcaccgcc cgcgaacgcc
     1141 ccttctcccc gggatcctcg cgagcccagg cgatgggtat gacctggatc gcgtgtacgg
     1201 cttccgagga tgccgctttt ctcaatccgg cctggcttgc taccgaggat gaacgcttca
     1261 ccatcatccg tctccgcacc gcagccaata ccgatggcac cgatctcctc ttcggccaga
     1321 ccggcgacga tctgatgacc ttcggttcct catccacccg gggacttccc ccggccggtt
     1381 tcatggacac catgaccggg tttgacgcgg tctacgatac gagcggcccc ctctttctct
     1441 cctacaaggg ggaggggttc ggtatcgggc tctattcgag ctcccgaggc agttttgcgg
     1501 ttcatggaac gaccctgccc actgtcaatc ttgaacacga ctttgatatc ggcaccatcg
     1561 ccggtttggc catccctata accttaaacg aagacacggg actccagctc ctgatcgggg
     1621 ggaccatcaa atacctgatc cggatccgtc acatttccag cgacgttccc ctgacggagg
     1681 cttcccgtct ggtcgatccc tttgcatttt cgggagactt ccggatcggg caggccatcg
     1741 gttccgatct gggtatcacc tggcgctcgc cctatttttc tgtcgggatt gtctggtacg
     1801 actggttcgg cacaaccctg tcctggaccg aatacagcgg ggaattcaaa accaagggga
     1861 catccctggt cgacacacac atcaccccca tgatcggggc cggcgtcgcc tggaccccgc
     1921 ccgactttct cggcctgccc tcttgggtct tttccaacac ccagatcgcg attgatgtca
     1981 ggggatttct cgaggaggag gactcgttct tcaaaatgct ccacgtcgga ttcgagagca
     2041 cattcctcca ctttgccaca atccgtctcg gtctgaacgc cggatacccg acctgcgggc
     2101 tagcctttct cctcttcggc agtctccata tggagtacac aatcgccacc gaggaacgcg
     2161 gccgtctccc cgggcaggac ccggtcaccc tgcataccct gtcgtttacc ttcatgctct
     2221 gaccgggcaa tcgcccatca gggcaagagc acttcttcaa caataaacgg aagataccgg
     2281 aaggccagtg cgcccttgcg gacccagacc ctcatcaatg gcaccgcttt tccgtcaacg
     2341 gtcaccatgg tctcgacctg ttccatcctg tcgacatccc tcagcgcgaa atccagaaaa
     2401 gtcggcgcac ccgagtccac tccgcaggcc agcaccatcc gctcttccga atccctgtgc
     2461 ttgttcggca ccccgatgaa ggcaacatgg gtttcgttgc acaccttgac ccgtgtatag
     2521 cggtctatct cgaaccgcgg tgcctcttcc agacatttcc acccgtcatt cactcccggc
     2581 ctccttgctc ccattcgaca tcacgtatcc gcagaataaa atataattcg gatccggtca
     2641 acaccccgga caacccgata cctgcttcaa aacgcactgc cattccggca gccaagcctg
     2701 tcttctccaa ccacccttct tgtctctcac caataaaaag gactccccgt ttttggggag
     2761 tccgctgctt aacaatatcc ggtcagatca gaatgttgac ggtgtcccgg tgttcgacgg
     2821 atcgt
//
LOCUS       DAOSHE010000265         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33943_length_2824_cov_3.068256, whole genome shotgun sequence.
ACCESSION   DAOSHE010000265 DAOSHE010000000
VERSION     DAOSHE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33943_length_2824_cov_3.068256"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PLD82_07450"
     CDS             complement(<1..1482)
                     /locus_tag="PLD82_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65256.1"
                     /translation="MEDYSVCHQTFGEGDGNDGLLHAAHALCSLFMPFASLLADADRL
                     SLPEALRRLTTHLREYFACGLVRILVSTDSGANIEDMRELASFAARRIYDTAMPLNHE
                     LFPAITGSRSPFQSPAKLLPEAGPREGDILSRISNEDCRLIILSLDENAVGLLVLANL
                     PASVHSRLSAEEGWLASRLSELIARFISQQRRRLLSAIEARLLHAFSDELPYGACILS
                     GDPATAPLLITEVNPAACHLLGYSKDELLHQPVSKIQDPEATRDTLERISKLTDSHPH
                     IWEGRLSRHNTGVFPAEMTASRMRISGEDLILLVCRDITQRHRTEQALRDSEERYALA
                     ARATNDGLWDWDLASGVVYYGQRWYEMLGYTSRDIRPDLDTWYDLVHNEDLPRLKSAI
                     AAHLQGESECIEVEYRIRHKDGGWRWMICHGLAVRDQAGAVTRVAGSQSDITQRKHAE
                     FTLLHDAFHDPLTNLPNRSLFMDRLKLAMARSRRSNDSGFAILY"
     gene            1752..>2824
                     /locus_tag="PLD82_07455"
     CDS             1752..>2824
                     /locus_tag="PLD82_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG65257.1"
                     /translation="MSVDFEGLFESFVAESSEMLDRAEGSVLELEKGFNKEVVNDIFR
                     AFHTIKGDSGIFGLQEITDTAHALENMLDLMRAEKLEVSMERIDLLLTGIDRLRQMIA
                     AARVGPGGVQLSSDGDVKTLLDTLHKAIAGDRQVPVKKPQTIVRQNSVGEGGSFSLRV
                     PKRYRLDAEARGHYLSLVYVDLYAQGISSLLEIRQILDNLEDQGSLLMQGVLENMLPG
                     RGPGADGSLLPYYMVLATQGPVEPFLEHHGIGGKVLRVLHTPKGQNAVPVGGAAVPVD
                     LVEDDAGVSGEAGTGGATPAVPAPLLAGENAIRDDRNAEEGVLLDEGRGTAGMIVKMP
                     AKKTKQSAVEAPPSAMPPAEPKE"
BASE COUNT          600 a          712 c          948 g          564 t
ORIGIN      
        1 gtagagaatg gcaaatccgg agtcgttgct cctgcgggag cgggccatcg cgagcttgag
       61 acggtccata aagagcgagc gattggggag attcgtgagc ggatcgtgga aggcatcgtg
      121 caggagggta aattcagcgt gtttgcgttg ggtaatgtcg gactgcgagc cggcaacccg
      181 ggtgaccgca ccggcctggt cacggaccgc cagcccgtgg cagatcatcc agcgccagcc
      241 gccatccttg tgcctgatgc ggtattccac ttcgatgcat tcgctttcgc cctggagatg
      301 ggccgcgatg gcggacttca accgggggag gtcttcgttg tggacgagat cgtaccacgt
      361 gtcgagatcg gggcggatat cgcgggatgt gtagccgagc atctcgtacc agcgctggcc
      421 gtaatagacg acgccactgg caagatccca atcccagagc ccgtcattgg tggcccgcgc
      481 cgccagtgca tagcgttctt cgctgtcccg cagggcctgc tccgtccggt ggcgctgtgt
      541 gatgtcacgg cagaccagga gaatcaggtc ttcgccggag atccgcattc ggctggccgt
      601 catctcggcc ggaaagaccc cggtattgtg gcggctcagc cgcccttccc agatgtgcgg
      661 gtgggaatcg gtcagcttgg agatccgttc gagggtatcg cgggtcgctt cgggatcctg
      721 gattttcgat acaggctgat ggaggagctc gtctttggag tatccgagga ggtggcaggc
      781 cgccggattg acctccgtga tgagaagagg ggcggtcgcc ggatccccgc tcaggatgca
      841 ggcgccatag ggcaattcgt ccgaaaaagc gtgcaggagc cgggcttcga tagccgagag
      901 gaggcgccgg cgttgctggc tgataaagcg ggcaatcagt tcactcaagc gggaggccag
      961 ccagccttct tcggccgaga ggcgggaatg cacggatgcc gggagattgg ccaggacaag
     1021 aagaccgaca gcgttctcgt ccagggagag aatgatcagg cggcagtctt cgttcgagat
     1081 ccgggagagg atatcccctt cccgggggcc ggcttccggg aggagtttgg ccggggattg
     1141 aaaaggagag cgagatcccg tgatggccgg aaagagttcg tggttgaggg gcatggctgt
     1201 atcgtaaatc cgccgggcag caaaggatgc cagctcccgc atgtcttcaa tattcgctcc
     1261 cgagtccgtg ctgacaagga tcctgaccag cccgcaggca aaatactccc ggaggtgggt
     1321 ggtcagcctg cggagggcct caggcaggct gagcctgtcc gcatcggcga gaagggaggc
     1381 aaaaggcatg aagagggagc agagagcatg ggctgcatgg agcagtccat cattcccgtc
     1441 tccctcgccg aacgtctggt gacatacgct gtagtcttcc acgaaagttg ctccgccggc
     1501 atatcaagta gttggtatag agtaccctat ggtatgcgaa actgcaagta ctagtctacg
     1561 aaaatgaaat attcgggaaa atcggagcgg actccaaatc agtatttata cgtatttaca
     1621 gcagcggcgg aatagcgcaa gctggaggag gttcggctga tgaacaggtt ttcgtagggc
     1681 aaagactggt aacatcaacc agatatgata tactgactcc ggattgacgg tgtggggcag
     1741 gaggagatgc aatgtcggtg gatttcgagg gactcttcga gagttttgtg gctgagtcgt
     1801 ccgagatgct ggacagagcc gagggctccg tcctcgagct tgagaagggc ttcaataaag
     1861 aagtcgtgaa tgacattttc cgggcatttc atacgatcaa aggggattcc ggaatcttcg
     1921 gccttcagga aatcacggat acggcccatg cactggaaaa catgctggat ctgatgcggg
     1981 ctgaaaaact cgaagtttcc atggagcgga ttgatctcct gctgacaggg attgaccggc
     2041 tgagacagat gattgccgcc gcacgggttg gtccgggcgg tgtgcagctt tccagcgatg
     2101 gtgatgtcaa gacattgctg gataccctgc acaaggcgat tgccggggat cgtcaggttc
     2161 cggtgaaaaa gccccagacg attgtcaggc agaattccgt cggagaaggc gggagttttt
     2221 ccctcagggt tcccaagcgc tatcgtctcg atgccgaggc gagagggcac tatctcagcc
     2281 ttgtctacgt tgatctgtac gcgcagggaa tttcttcgct tctcgagatc aggcagattc
     2341 tcgacaacct cgaggatcag gggagcctgt tgatgcaggg tgtgctcgaa aacatgctgc
     2401 cgggacgtgg gccgggagcc gatggttcgc tcctcccgta ttacatggtg ttggcaacac
     2461 aaggtcctgt tgaaccattt ttggaacacc acgggattgg cgggaaagtc cttcgggtgc
     2521 tgcacacccc gaagggacag aatgccgttc cggtgggggg ggcggccgtt ccggtcgatc
     2581 tggtcgagga tgacgcgggt gtttccggag aagcggggac cggcggggcc acaccggctg
     2641 ttccggctcc gttgctggct ggggagaatg cgatccgtga cgacaggaat gccgaggagg
     2701 gtgttcttct cgatgaggga cggggaacgg caggcatgat tgtcaagatg cctgccaaaa
     2761 aaacaaaaca atccgcagtc gaagcgcctc cgtccgcgat gcctcccgct gaaccgaaag
     2821 aaag
//
LOCUS       DAOSHE010000266         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_33980_length_2822_cov_3.410553, whole genome shotgun sequence.
ACCESSION   DAOSHE010000266 DAOSHE010000000
VERSION     DAOSHE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33980_length_2822_cov_3.410553"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLD82_07460"
     CDS             complement(<1..918)
                     /locus_tag="PLD82_07460"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOG65258.1"
                     /translation="MAKKVRVLVIDDSAVVRQTLSDVLSADPEIEVIGTAADPFVAAE
                     RLKTEIPDVITLDVEMPRMDGLTFLKKLMTQHPLPVVMCSSLTDKGCETAIRALEYGA
                     VEIITKPKMGVRQFLEEARVQICDAVKAAASVKVKPVSEPKKPAAKLTADAMLSLPAT
                     KAMVKTTERIIAVGASTGGTEALRIYLEAMPKDCPGIVIVQHMPEHFTRAFSQRLNEI
                     CTIEIKEAADGDTVHPGRALIAPGNHHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLF
                     RSAARYAGPNAVGVIMTGMGDDGARGLLEM"
     gene            complement(918..1829)
                     /locus_tag="PLD82_07465"
     CDS             complement(918..1829)
                     /locus_tag="PLD82_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HOG65259.1"
                     /translation="MSVEAHKAGGPHGPSASSHPGPMRLTDREFKEFAALIYDRCGIN
                     LAPIKLIMLSSRLSKRLRVLSMDSFERYLDYVRTPEGLAQELVHLIDVVTTNKTEFFR
                     EPKHYEILTGKVLPELAARGRTRMIRVWSAGCSTGEEPYTLSMVFDRWCNNNGMSWAI
                     TASDISTKVLETAQKAVYQTRHIESIPDTYRREYLMRGKGRMAGMFRIVPELRKRIDF
                     MRVNLVDDAGFGLSQKFDIIFCRNVVIYFDRETQKKLFERFWRQLVPGGFLFIGLSET
                     LHRINDDFVSLSSSVYRKPALLPTEAP"
     gene            complement(1826..2326)
                     /locus_tag="PLD82_07470"
     CDS             complement(1826..2326)
                     /locus_tag="PLD82_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG65260.1"
                     /translation="MDENSMMQYLTFTLGAEDFALSIAQVREVLNYTTITTVPRMPEF
                     LRGVINLRGNVVPVIDLRLKLGMEATQRTADTCIVIVEMQVEGDLVQMGALADSVKEV
                     IDLDRSDIMPPPKLGTSLKSEFIQGMARQDEKFLILLNIDRILSDDEMSTVKTAGHAQ
                     AVKEKA"
     gene            complement(2338..>2822)
                     /locus_tag="PLD82_07475"
     CDS             complement(2338..>2822)
                     /locus_tag="PLD82_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65261.1"
                     /translation="AAKEISSLSVHSVEIAERAGKLIENIVPGIQKTAELVQEINASS
                     AEQSEGIKQVTKAVQQLDQVIQQNASATEEMASTSEELSSQAQQLKEAAGFFKVENSG
                     RSQVSAAGPSHARVSAHTTHQTQHGFTSSKKFETKTAEKKGGVDLDMSDPDDAEFERY
                     "
BASE COUNT          523 a          801 c          852 g          646 t
ORIGIN      
        1 catttcgagg agcccccggg caccatcgtc acccatcccg gtcatgatca ccccgacggc
       61 attcgggccg gcatagcgcg ccgcggaacg gaagaggaca tcgacggacg gccggtggcg
      121 ggagacgagt ggcccgtcct tgatctcgac ataatatcgg gcaccgcttc gcttgagaag
      181 catgtgatgg ttgcccggtg cgatcagggc ccgtcccggg tggacggtgt ctccgtcggc
      241 ggcttccttg atttcgatgg tgcagatctc gttcaggcgc tgtgaaaagg ccctcgtaaa
      301 atgctcgggc atgtgctgga cgatcacgat cccggggcag tccttgggca tcgcctcgag
      361 gtagatccgg agggcctccg ttccaccggt cgatgcgccg acggcgatga tccgctccgt
      421 tgttttgacc atggccttgg tggccgggag gctgagcatg gcatcggccg tgagtttcgc
      481 ggcaggcttt tttggttcgg agaccggctt gactttgacg gaggccgcgg ccttgacagc
      541 gtcgcagatc tggacacgtg cttcctcgag gaactggcgg acgcccatct tcggtttggt
      601 gatgatttcc accgcgccgt attcaagggc gcggatggcg gtttcgcatc ctttgtccgt
      661 caggctggaa cacattacga ccggcagggg atgctgcgtc atcagctttt tgaggaaggt
      721 caggccatcc atgcggggca tctcgacatc cagagtgatc acatcgggga tttccgtttt
      781 gagccgttcg gcggcgacaa agggatccgc cgcagtcccg atgacttcga tttcgggatc
      841 cgccgagaga acgtccgaaa gcgtctgtcg gacgaccgcg gaatcatcaa tcacgagtac
      901 acggaccttc tttgccatca gggtgcctcc gtcggaagta gtgccggttt gcgatagacc
      961 gagctggaca acgagacaaa gtcgtcattg atccggtgca gggtctcgga gagaccgata
     1021 aagagaaacc cgcccggcac gagttgccgc cagaaacgct caaagagttt tttctgggtc
     1081 tccctgtcga aatagataac gacattccgg caaaaaataa tatcgaattt ttgcgataat
     1141 ccgaatcccg cgtcatcgac gagattgacg cgcatgaagt cgatccgctt gcggagttcg
     1201 ggaacaatgc ggaacattcc ggccatgcgg cctttccccc gcatgagata ttcccgcctg
     1261 taggtgtccg ggatgctttc aatatggcgt gtctggtaga cggctttttg ggcggtctcc
     1321 aggaccttgg tggagatgtc gctggcggtg atcgcccagg acatgccgtt gttgttgcac
     1381 cagcggtcga agaccatgga gagggtgtag ggttcctcgc cggtggaaca tcccgcactc
     1441 cagacccgga tcatcctggt gcgtccccgg gcagcgagtt cgggaaggac cttgcccgtc
     1501 aaaatctcgt aatgcttcgg ctccctgaaa aactccgtct tgttggtggt gacaacatcg
     1561 atcaggtgga ccaattcctg tgccagccct tcaggagtgc ggacgtagtc gagatagcgt
     1621 tcgaaggaat ccatcgagag gacccgcagc cgcttggaga ggcgggagga gagcatgatc
     1681 agcttgattg gcgccagatt gatcccgcaa cgatcgtaga tcagtgcggc aaattccttg
     1741 aactcccggt cagtcaggcg catcgggccc ggatggctgg aagcggacgg gccgtgtggc
     1801 ccgcccgctt tgtgcgcttc cacgctcatg ccttttcctt gacggcctgg gcatgacccg
     1861 ctgttttgac cgtggacatc tcgtcatccg acaggatccg gtcaatgttg aggaggatca
     1921 ggaatttctc gtcctggcgg gccatccctt ggatgaactc gcttttaagg cttgtcccga
     1981 gtttcggtgg tggcatgatg tccgaacgat caagatcgat cacctccttg accgagtctg
     2041 ccaaggcccc catctggaca agatcccctt caacctgcat ctcgacaatc acgatacatg
     2101 tgtcggccgt gcgttgcgtg gcctccatcc ccagcttgag ccggagatcg atcaccggga
     2161 cgacatttcc ccggagattg atcactcccc gcaaaaattc cggcatgcgg ggaaccgtcg
     2221 tgatcgtcgt gtaattcagg acctcacgga cctgggcgat agagagggca aagtcctccg
     2281 cgcccagcgt aaaggtcagg tactgcatca tgctgttttc gtccatcgcc atccccctca
     2341 ataccgttcg aattcggcat cgtcgggatc gctcatgtca agatcaacgc cgcctttttt
     2401 ttcagccgtt ttcgtctcga atttttttga agatgtgaaa ccatgctgtg tctggtgcgt
     2461 tgtgtgtgcc gagaccctgg cgtgggacgg gccggcagcg gatacctggc tcctgccact
     2521 gttctcgact ttgaagaaac cggccgcctc cttgagttgc tgggcctgac tggagagctc
     2581 ttcgctggtc gaggccatct cttcggtcgc gctcgcattc tgctggatga cctggtcgag
     2641 ctgttgcacg gccttggtca cctgcttgat tccttcggac tgctcggcac tggatgcgtt
     2701 gatctcctgg acgagctcgg ctgttttctg gattcccgga acgatgttct cgatcagctt
     2761 gccggcgcgt tcggcgattt cgacactgtg caccgaaagg ctggatatct ccttggctgc
     2821 ag
//
LOCUS       DAOSHE010000267         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34011_length_2820_cov_3.569620, whole genome shotgun sequence.
ACCESSION   DAOSHE010000267 DAOSHE010000000
VERSION     DAOSHE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34011_length_2820_cov_3.569620"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLD82_07480"
     CDS             complement(<1..493)
                     /locus_tag="PLD82_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65262.1"
                     /translation="MLHRLQNRIPLIISILGALYIVSPILYYVYYASKTGPGVSFTDV
                     GAVLRYAGDAYFIQFVLVMLVTPIVGVGLVLKRKWGLIAFFVYGIALIAQSVVNYVSR
                     PESFSAPAVIGNIVVICVLLLFIRLELRAPFYRPDWQIRGRRFKCALPVAWKKAGEQA
                     SVRS"
     gene            720..1985
                     /locus_tag="PLD82_07485"
     CDS             720..1985
                     /locus_tag="PLD82_07485"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOG65263.1"
                     /translation="MPQSVSPSPFTWFENPLSGGCDTLDVARELYPEGSDRLPWDFHR
                     HLPGYRMSPLIGLHNLATMLGLGGIWVKDESVRMNLGSFKVLGGSYAIYRFIKKHLGL
                     EDREFTFEELTSAAMKARYGNLVFATATDGNHGRGVAWAATQLGLRSIVYVHKGTSQA
                     RIDAIAKNGAETRIIDGTYDDAVRQVNLDAQKNGWQVISDTSWEGYEDIPRWVMQGYS
                     TLFNEAQLQLSAQGISAPTHVFVQAGVGALAASAIGFYHQRCPDNPPKSVVVEPDKAA
                     CLWLSAREGQGKPLSFKGELDTIMAGLACGDPSPLAWRILSRCTDVFAVCPDYVAAKG
                     MRVYGVPLAGDPFVVSGESGAVTLGSIMFLMAMPEAAGLREKLGLDRSSQILLINTEG
                     NTDPDDFRRIVWEGGDPVPEEFKFYKQDP"
     gene            2235..>2820
                     /locus_tag="PLD82_07490"
     CDS             2235..>2820
                     /locus_tag="PLD82_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG65264.1"
                     /translation="MKVLLADKFEAAGIEKLKSLGCDVISEPDLSAETLAEGLVKHDP
                     DVLIVRSTKVQKPALEAAKTLSLIIRAGAGYDTIDINTASARGIFVANCPGKNAIAVA
                     ELAFGFVLSCDRRIMDATRDIRAGKWKKGLYSKAQGLFGRTMGVIGRGQIAQALIDRA
                     KGFGMEVMVWSRSLSEADAEAMGVARAASLVDLAK"
BASE COUNT          571 a          929 c          816 g          504 t
ORIGIN      
        1 tggaccgcac ggacgcttgc tcccctgcct tcttccatgc caccggaaga gcgcacttga
       61 agcgccgtcc acggatctgc cagtccggcc ggtagaaggg tgcccgcaac tcgagccgga
      121 tgaagaggag caggacacag atgaccacaa tattgccgat gactgccgga gcgctgaaac
      181 tctcgggacg ggacacgtaa ttgacaacac tctgggcaat cagggcgatt ccgtagacaa
      241 aaaacgcgat caacccccat ttccgcttga gcaccagtcc gactccgacg atcggggtga
      301 ccagcatgac gaggacgaac tggatgaagt aggcgtcgcc ggcataacgc aagacggccc
      361 cgacgtcggt gaacgacacg cccggcccgg tcttgctggc gtagtagacg tagtacagaa
      421 tgggagaaac gatgtacaga gcacccaaaa tcgaaatgat caggggaatg cggttttgca
      481 gacgatgcaa catggcggga acctccgatg gttgactctc cccgtacagt accagtcaat
      541 cggctcctcc gcaagacaaa tcatgaattc ggcatcagtc gccgcatccg ttctcccggc
      601 cggaacacag cgtgcattgg caggcaactc gcatctcgcc ccggccttga cccggccgga
      661 ttccgggtgt agactggtgt cttcgagtct cttttaccgt ctgcaacaag gagtcaccca
      721 tgccccaatc cgtctcgcct tcccccttca cctggttcga aaaccccctt tccggcggtt
      781 gcgataccct cgacgttgcc cgtgaacttt accccgaggg cagcgaccgg ctcccctggg
      841 atttccatcg ccatctcccg ggctatcgga tgagtcccct gatcggcctg cacaacctgg
      901 ccaccatgct cggtctgggc gggatctggg tcaaggacga gtcggtccgg atgaacctcg
      961 ggtccttcaa ggtcctcggt ggctcctacg ccatctaccg cttcatcaaa aagcacctcg
     1021 gccttgaaga ccgcgaattc acgtttgagg aactgacatc cgccgccatg aaggcccgct
     1081 acgggaacct tgtctttgcg acggccacgg acgggaacca tggccgcggt gtggcctggg
     1141 ccgcaacaca actcggactg cgctccatcg tctacgtcca caagggaacc tcccaagccc
     1201 ggattgacgc catcgccaaa aacggcgccg agacgcgcat tatcgacggg acctatgacg
     1261 acgcggtccg ccaggtcaac ctggacgcgc aaaagaacgg ctggcaggtc atctccgata
     1321 cctcatggga gggatacgaa gatattcccc gctgggtgat gcagggctat tccacgctct
     1381 tcaacgaagc gcaactccag ctctcggcac aggggatcag cgcaccgacc catgtcttcg
     1441 tccaggccgg tgtcggcgcc ctggcggcct cggccatcgg cttttaccac cagcgctgcc
     1501 cggacaatcc ccccaaaagc gttgttgtcg aaccggacaa ggccgcctgc ctctggctct
     1561 cggcccgcga aggacaggga aagccgctct ccttcaaagg ggaactagac acgatcatgg
     1621 ccggactggc ctgtggcgac cccagcccgc tggcctggcg catcctctcc cgctgtaccg
     1681 atgtttttgc ggtctgtccc gactacgtgg ccgccaaggg catgcgggtc tacggtgttc
     1741 ccctggccgg cgaccccttt gtcgtctccg gtgaatccgg tgcggtcacc ctcgggtcga
     1801 tcatgttcct gatggccatg cccgaagccg ccggcctgcg agagaagctg ggcctcgacc
     1861 gctccagcca gatcctcctg atcaacaccg aggggaatac cgaccccgat gacttcaggc
     1921 gcatcgtctg ggagggcggg gatccggttc ccgaggagtt caagttctac aagcaggatc
     1981 cgtaagcgga tgcccttcat gccggtcagg ccctgccttg accggcctgc acctggccgg
     2041 gagacttgcg ctcccgccgg gaaggctccg tcgggcggac cagtaccgga cgccattaac
     2101 cagagctgac cagagcagcc tcgcgccgcg tttcgcttgc gtaccgaccc tgtccatgct
     2161 atactcctgt cagttgcact cgccccccgc aaccgggagc acaaccaata cactacctat
     2221 tccaaggaga aaccatgaaa gtccttctgg cagacaaatt tgaagccgca ggcatcgaga
     2281 aactcaagag cctcggctgt gacgtcatct ccgagccgga tctctcggcc gaaacgcttg
     2341 ctgaaggtct cgtcaagcat gacccggacg tcctgatcgt ccgctcgaca aaggtgcaaa
     2401 agccggccct cgaagcggcc aagacactca gcctgatcat ccgggctggc gccgggtacg
     2461 atacgatcga catcaacacc gccagcgccc gggggatctt cgtggccaac tgtcccggga
     2521 aaaacgcgat cgccgtcgcc gagctggcct tcggcttcgt cctttcctgc gaccggcgca
     2581 tcatggatgc cacccgtgac attcgggccg gcaaatggaa aaagggactg tattccaagg
     2641 cccagggtct ctttggccgg acgatgggtg tgatcggtcg cggacagatc gcccaggccc
     2701 tgatcgaccg ggccaagggt ttcggcatgg aggtcatggt ctggagccgc tccctgagcg
     2761 aggcggatgc cgaagccatg ggtgtcgcac gggccgccag tctggtcgat ctcgccaagg
//
LOCUS       DAOSHE010000268         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34075_length_2817_cov_2.874004, whole genome shotgun sequence.
ACCESSION   DAOSHE010000268 DAOSHE010000000
VERSION     DAOSHE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34075_length_2817_cov_2.874004"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLD82_07495"
     CDS             complement(<1..855)
                     /locus_tag="PLD82_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG65265.1"
                     /translation="MPPEKDPPVAPSSKEAMNALISKRIDEAFTRNALAVNATLDNLG
                     AISEQKNINLDAMDRHERADAVKILNMVEQARLETERILNGPQLAKEPLAGRMQQRAS
                     TVDTVYGGLLSGRQEAVKILETMVSGFIDDTGPNQDASLLLKDIISQEQRFLANHCLN
                     VAVISLAAAIELNKMMTRKLDDPGIQKDINLLRAIRLKTFTREELIKLGFAAFVHDIY
                     YKKVFPHLKHTDVLNALADRSNVERHASESYHLMRQFEMDYHVNKAVLQHHERIDGTG
                     SPDGISERL"
     gene            1137..>2817
                     /locus_tag="PLD82_07500"
     CDS             1137..>2817
                     /locus_tag="PLD82_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65266.1"
                     /translation="MWPFSKRSIQKPGEDDQDGGRSLRYDQVREAENKRYLTELVNAR
                     RPPEIQKRLLEVLKYEPSLTKKIDIVERLDRLGVDTLGWYEQRITLVKAGAQRAQTEK
                     ARKSAYFFQSPEYRALSVESKELLRGLEPPSFFRFATREFRKISAYGRRTGLLKASLF
                     PLRHELAGSLLTWCNDEMRMYAAALEHSLARVVERGWATLDIHSYNLLVRFEALVRGW
                     LAESPRLSHKNTSRLVWLKLGPLARIWFSITRVEGQASLALDGLAQFLKANGADGAQI
                     RRQLSAAEAILDAERVGVSFGRAVLAVWMLLSRRFFTLPALMEKLKPEPVSRETWVCP
                     AETLEMIQAFIQARRSERDEFSRTILLLDEIGILEPDLMDERCMHEIWCLYAEALHVL
                     PEAGRTAASQARQTLAVDITALFERATSNLSYCITILMRDRLLAEHTLAGERDLLDRA
                     LSAMRSIRQVRERLVIPMATYDAWFRKNTAPGDLSDAERGFAQAIADAGAAAAHIGLK
                     VQQLVTQAAGLDHLAGARARDASGILADLSEDLREPQVFAGVSLRESLARVQ"
BASE COUNT          565 a          753 c          856 g          643 t
ORIGIN      
        1 gaggcgttcg gaaatcccgt ccggcgaacc tgttccgtca atccgttcgt gatgctggag
       61 gaccgctttg ttgacgtggt agtccatctc gaactggcgc ataaggtggt agctttcgga
      121 ggcgtggcgc tcgacattgc tgcggtcagc cagcgcattg agcacatcgg tgtgtttgag
      181 atggggaaag acttttttat agtagatatc atggacgaaa gcagcgaagc cgagtttgat
      241 gagctcttcc cgggtaaagg tcttgagacg gatggctcgc agaaggttga tgtctttttg
      301 aatccccgga tcatcgagct tgcgggtcat cattttgttg agttcgatgg ctgcggccag
      361 tgagatcacg gcaacattca ggcagtggtt ggccaaaaag cgctgttcct ggctgatgat
      421 gtccttgagc aggagagagg catcctgatt gggtccggta tcgtcaataa aaccagagac
      481 catcgtttcg aggatcttga cggcttcctg tcgaccgctt aacaagcctc catagacggt
      541 gtcgaccgta ctggcacgtt gctgcatccg accggccagt ggttccttgg cgagctgcgg
      601 gccgtttaaa atgcgttctg tctcgagacg ggcctgttcg accatattga ggatcttgac
      661 ggcatccgcc cgctcgtggc ggtccatcgc atccagattg atattttttt gttcgctgat
      721 ggctccgaga ttgtcaaggg ttgcattgac ggcaagagca ttccgtgtaa aggcctcatc
      781 aatgcgcttg gaaatcaggg cattcatggc ctcctttgag gatggggcaa cgggcggatc
      841 cttttcgggg ggcatctcgg gcacagaaca ctccttgtat ggtgtctcta cacactgatc
      901 cggaaatctc taaaacattg tcggtatttc aggcaggtgt cttccgtggc tgggtgaaaa
      961 gcgtattggt tgggagagca ccgtacaaca atatgtatct taatagaagt ctccctataa
     1021 atgcaacagt tttcaatttt ggccgggcaa catgcctttg tcagtttctt ttgtatggcg
     1081 aaaattgcag ccgtcatgta gactgtgaag acgacaaggg agggagcagg acagctatgt
     1141 ggccgttctc gaaacgcagc attcaaaaac ccggggaaga tgaccaggat ggtggtcgca
     1201 gtctgcgcta tgatcaggtc cgtgaagccg aaaacaagcg ctacctcacc gaacttgtca
     1261 atgcccggag accacccgaa atccagaaac gccttctcga ggttctcaag tacgagccca
     1321 gcttgaccaa aaagatcgat attgtcgagc ggcttgaccg gttgggtgtg gatacccttg
     1381 gctggtatga acagcggatc accctggtca aagcgggagc gcagcgggca cagaccgaaa
     1441 aggccagaaa gtctgcctat tttttccaaa gtccggagta tcgtgcactt tcggtcgaat
     1501 cgaaggaact tctgcggggg cttgaaccac cgtctttttt ccgttttgcc acccgggaat
     1561 tcaggaagat ttcggcgtac gggagacgga ccggactgct caaggcaagc ctctttccat
     1621 tgcggcatga actggccggg agtcttctga cgtggtgcaa tgacgaaatg cggatgtatg
     1681 cagcggccct cgagcactct ctggcacggg tagtcgaacg gggttgggcc acgcttgata
     1741 tccattccta caatcttctc gtgcggtttg aggcactggt gcggggttgg ctggccgaat
     1801 cccccagact ctcgcacaaa aacacctcgc gtctggtctg gctcaagctc ggcccgctcg
     1861 cgaggatctg gttttccatt acacgggtcg agggacaggc cagtctcgcg ctggacggtt
     1921 tggcccagtt cctcaaggcc aatggagcgg atggtgcaca gatccggcgg cagctctcgg
     1981 cagccgaagc cattcttgat gcagagcggg tcggggtctc attcgggcgt gccgttttgg
     2041 ccgtctggat gcttctctcc cggcggttct tcaccctgcc ggccctgatg gaaaagctca
     2101 aacccgaacc ggtctcccgg gagacctggg tctgcccggc ggagaccctc gagatgatcc
     2161 aggccttcat tcaggcccgg cgttccgaga gagatgaatt ctcgcgcacc atccttttgc
     2221 ttgacgagat cgggattctg gaaccggacc tgatggatga gcggtgcatg cacgagatct
     2281 ggtgcctcta tgccgaagcc ctgcatgtac tgcccgaggc tggccgcacg gctgcgtcgc
     2341 aggcccggca gacactggcc gtggatatca cggccctctt tgagcgggca acctccaatc
     2401 tttcgtattg catcacgatt ctgatgcggg acaggttgct tgccgaacat acgttggccg
     2461 gtgagcggga cttgttggac agggcgctct cggccatgcg ttccatccgg caggtacgtg
     2521 aacgcctcgt gattcccatg gcgacctacg atgcctggtt ccggaagaac accgcccccg
     2581 gagacctctc ggatgccgaa cgcgggtttg cccaggcgat cgccgacgca ggcgcggcag
     2641 cggcacatat cggactcaag gtccagcaat tggtgacgca ggccgccgga ctggaccatc
     2701 tggccggtgc cagagcccgg gatgcctcgg gcatcctcgc ggatctgtct gaggatctgc
     2761 gtgagcccca ggtgtttgcg ggagtcagtc tgcgcgagtc gctcgcccgg gtccagg
//
LOCUS       DAOSHE010000269         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34254_length_2807_cov_2.850654, whole genome shotgun sequence.
ACCESSION   DAOSHE010000269 DAOSHE010000000
VERSION     DAOSHE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34254_length_2807_cov_2.850654"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..609)
                     /locus_tag="PLD82_07505"
     CDS             complement(334..609)
                     /locus_tag="PLD82_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOG65267.1"
                     /translation="MKIVFTTFLLVFFAELGDKTQIAALSMAASGGKMLEVFIGASLA
                     LVAATLMAVFLGRWVSRHVRPEILKRVSAILFVATGGWLFLKAFGAL"
     gene            complement(612..1370)
                     /locus_tag="PLD82_07510"
     CDS             complement(612..1370)
                     /locus_tag="PLD82_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOG65268.1"
                     /translation="MAEVDCATIASVFVLVFLAELGDKTQLVAFSMTATARSPWPIFF
                     GASGALVLSSLLAALLGGAAGNAFGESMVIKIVSASLFVIFGLLMLFEREDPPVKKAF
                     LDALLLESQGARLMTKILARYPHPDAVRISREISADEASHEALFRLLLKKKRFFADDI
                     NHDCGLPGLSAGLHIAKGLKRMPAREALAELIRIEQACGRFYAFLARHIAESHHDEAA
                     LGADLEGMVQEEARHASLLSTLLATDGSGKGVGK"
     gene            1469..1864
                     /locus_tag="PLD82_07515"
     CDS             1469..1864
                     /locus_tag="PLD82_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65269.1"
                     /translation="MKILIVEDEASSRHLLKSLLSGYGTCELAEDGPVAVEMFKAALT
                     AGEQFDLVTLDIKLPTMDGMEVLRNIRRIESEQGIQIGDGTKIIMTTALSDHKKVIEA
                     FNEQCEVYLVKPIEKAKLVEHMRKLKLID"
     gene            complement(2155..>2807)
                     /locus_tag="PLD82_07520"
     CDS             complement(2155..>2807)
                     /locus_tag="PLD82_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOG65270.1"
                     /translation="RRESGGVGQPGREQNAAPQRLSMTQTTNKVLAIGTSTGGTEALR
                     KILPRLPGNAPGIVIVQHMPEHFTRSFAQHLNEMCAIDVKEAEDGDTVAPGKALIAPG
                     NYHMLLQRSGAMYYVQVKQGPLVNRHRPSVDVLFKSVARYAGANAVGVIMTGMGADGA
                     AGLKEMREAGARTVAQDEASCVVFGMPKEAIAMGGAEFVEPLARIPERMLELAGQG"
BASE COUNT          723 a          837 c          698 g          549 t
ORIGIN      
        1 cctacggcga gacgaaaaac tacatcaaac tcgttctctc aaacctgatg atctaccggt
       61 atctctatca ataagggttt tcgggttctt tttttggtga acagactcag gccatgaatg
      121 ccctcttcag cacattccga acaacatggc aacacagtcg gtgctcgctc tgactcttcc
      181 acttgctgac agacaaagtc tacgcttgcg agtacttcgc tcgccaagag taacctacgg
      241 ccggggcgct catacagcat ggagtctgag gcatgaggtt gttcatcaaa aaaaactaca
      301 agccagggca cgcgattgta acgaacaaag ggattacagc gcgccaaagg ccttgagaaa
      361 gagccagccg cccgtggcga caaacaggat cgctgaaacg cgtttgagga tttctggcct
      421 gacgtgcctg gagacccaac ggccaaggaa taccgccatc agggttgcag caacaagggc
      481 cagggaggct ccgataaaaa cctccagcat ctttcctcct gaagcggcca tggagagcgc
      541 ggcaatctgt gtcttgtcac ccagctcggc aaaaaaaaca agaagaaaag ttgtgaaaac
      601 gatcttcatg ctcattttcc cacccctttg ccggatccgt ctgttgccag cagcgtcgag
      661 agaagagacg cgtgtcttgc ttcctcttga accatcccct caagatccgc gccaagcgcc
      721 gcttcatcgt ggtgggattc cgcgatatgc cgggcaagaa aggcataaaa ccgcccacag
      781 gcctgctcga tcctgatcag ctcggccaaa gcctcccgtg ctggcatccg tttcaaaccc
      841 tttgcgatat gcaaaccggc agaaagcccg ggcaagccac aatcgtggtt gatgtcatcg
      901 gcaaaaaacc gttttttttt gagcagcaaa cgaaacaggg cctcgtgcga agcctcgtct
      961 gcggaaatct cccgactgat acgaacggca tcgggatgcg gataccgcgc aagaatcttc
     1021 gtcatcagcc ttgctccctg gctttcaagc aggagcgcgt cgagaaaggc ttttttgaca
     1081 ggaggatctt cacgttcaaa cagcatcaag aggccgaaga tgacaaacag ggaggccgag
     1141 acaatcttga tgaccatgct ttcaccaaag gcgttccccg cggctcctcc aagcagggca
     1201 gccagcaggg aagagagcac aagcgccccg gacgctccaa aaaaaatggg ccagggggag
     1261 cgggcggtcg cagtcatcga aaacgcgacg agttgtgtct tgtcccccaa ttcagccaaa
     1321 aaaacgagaa caaaaacaga tgctatcgtc gcacaatcca cttctgccac ctgcctttcc
     1381 ggaatcaaga atgaaaatat agtattgtca tgtgttcttt ttcgggataa catgatacaa
     1441 caacttgtgc gaccggaggg tataccatat gaagatcctg atcgtcgaag acgaggcatc
     1501 gagccgacat ctcctcaaat cgctgctgtc cggctacgga acatgcgaac tcgccgaaga
     1561 tggccccgtg gcagtcgaga tgttcaaagc ggctctgacc gccggagaac agtttgacct
     1621 cgtcacactg gacatcaaac tcccgacgat ggacggcatg gaagtcttgc ggaacatccg
     1681 gcgcatcgaa tcggaacagg ggatccagat cggtgacggc acgaagatca tcatgacgac
     1741 cgccctctcc gaccataaaa aagtcattga agctttcaac gagcaatgcg aagtctatct
     1801 tgtcaaaccg atagaaaagg ccaaactggt cgagcacatg cgcaagctca agctgatcga
     1861 ttaacatccc tttttcaagc ttgcagatcc aaaaacacga tccacgggta tgacaaaact
     1921 tttcactttc ataccctgtg gcctcgctag acaagcctgg ccactactcg ctaaaaacgc
     1981 ctctgcgcga cagtaccgca aagccgatac ccaccccgac acagcacacc acccatgccg
     2041 ggagaacaat ccatcaggaa tactctccga aagacttcag cggttaaacc cgggcaagat
     2101 gttccgtgga accttcaaaa ataccatgat gaaaaaaaac accatccagg agaatcaccc
     2161 ctgaccggcc agctccagca tccgttcagg gatcctggcc agaggctcca cgaactccgc
     2221 accacccatg gcaatggcct ccttgggcat cccgaaaaca acacagctcg cctcgtcctg
     2281 cgccacagtc cttgcgccag cctcccgcat ctccttgagc ccggccgccc cgtcagcgcc
     2341 cattcccgtc atgatgacac cgacagcatt ggccccggcg tagcgcgcca cggacttgaa
     2401 gagcacatcc acagatggcc tgtgcctgtt gacaaggggc ccctgcttga cttggacgta
     2461 gtacatggcc cctgaccgct gcaaaagcat gtgatagttt ccgggcgcaa tcaaggcttt
     2521 cccgggggcg accgtatccc cgtcctcggc ttccttgaca tcaatggcac acatctcgtt
     2581 gaggtgctgg gcaaaagaac gggtaaaatg ctcgggcatg tgctggacaa tcacaatccc
     2641 cggagcattc cctggaagcc gcggcaaaat cttgcgcaag gcttcggtcc ccccggtcga
     2701 tgttccgatt gccaggactt tattggtcgt ctgtgtcatc gagagtcgtt ggggtgcggc
     2761 attctgttcg cggcccggct gacccacgcc cccgctttcc ctgcgct
//
LOCUS       DAOSHE010000270         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34318_length_2803_cov_3.975619, whole genome shotgun sequence.
ACCESSION   DAOSHE010000270 DAOSHE010000000
VERSION     DAOSHE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34318_length_2803_cov_3.975619"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1402
                     /locus_tag="PLD82_07525"
     CDS             263..1402
                     /locus_tag="PLD82_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG65271.1"
                     /translation="MKFRPGRLVLFNIVLASLLVGSLVTTMVWSGCDGNSHTLSAKTA
                     NETVLGDKEALNAALAMQRAFRNIAKSVGPAVVSVNVVQTVAQGNPYNELFGDDLFRY
                     FFGDRGQGQQRPQQRRKSVSAGSGFIIDKKGYILSNFHVVKQASEITVVLIDGREFKA
                     TVVGTDSATDIALLKIDPSGEIPVVALGDSDKLEVGDWTIAIGNPFNLPGTFTVGVVS
                     AKSRGDQIGAPYQNFIQTDTAINPGNSGGPLVNISGEVVGINTMIYTRTGGSLGIGFA
                     IPVNIAKNVVSSLIKDGKFERGYIGIYPGPIDDGMRTALKLPKESGVIVNSVIADGPA
                     ARAGMKDGDVILAIDGQDIISVPQLMRIVAEVPAGKTVPFVIQRQ"
     gene            1505..1777
                     /locus_tag="PLD82_07530"
     CDS             1505..1777
                     /locus_tag="PLD82_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG65272.1"
                     /translation="MQIAPVSSINPLQLRRLGIDPNETGVVITGFSENAPESELAPGD
                     LIKEINYVAINSIDDYRRFVEANKGKKSFIFKVKRQGQMLWVGMNA"
     gene            1959..>2803
                     /locus_tag="PLD82_07535"
     CDS             1959..>2803
                     /locus_tag="PLD82_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65273.1"
                     /translation="MAETPTFQRQNPYVALPTRMFLTTEGLRLFAAKPDQLKRVKNRM
                     GIVKEAIESDRYNALLVQKLVLNSCIEEVYVRLPNLLQYRQDLISTNNLILYAILYKK
                     LSPSLADMLCQSPVVQAYNRKNPKYAIVGLNMISRQKIDAIRMEKPQLFEAMESEIME
                     EVKARIKRQPISDEDKQIRERSLEKFIAWIDKRIWYIYLIIYQTQLKRELVRSFTDML
                     VTYLGRTQIATHLSSLLMEFVQNAEKAHFERIVIRKRFAAEKMADSFIRDRKNREAVI
                     AEAVR"
BASE COUNT          681 a          734 c          753 g          635 t
ORIGIN      
        1 caggcctgct gaagttctga tcgaggaagg ccgtgaccgt ctcgtccgga agcgcgagag
       61 catcgacgat atcctgagac tccagacctg gtgatgctgt gccaaaatgg cacgagcgtg
      121 atccgcgtgg ccttttcggc acatccagta cttgacattt gtgtcggtaa ctttctatat
      181 tctcataata aacagtgttg gcacgttttt tgattatctc gtggtgaagt ttgtttgata
      241 ctccaaggag caacctatgc gaatgaaatt tcgacccggt agacttgtcc tcttcaacat
      301 tgttctcgcc tcgcttctgg tggggagtct tgtcacaacg atggtctggt ccggttgtga
      361 tggaaacagc cacactcttt cggcaaagac ggccaatgaa acagtcttgg gtgacaaaga
      421 agcgctcaat gcggcactgg ccatgcagcg ggctttccgg aatattgcca aatcagtcgg
      481 gccggccgtt gtcagcgtga atgttgtcca gacggtggcc caggggaatc cctacaacga
      541 actttttggt gatgacctgt tcaggtattt cttcggtgat cgtggtcagg gacagcaacg
      601 accacagcaa cgccgcaaga gtgtttccgc cggttccggt ttcatcattg ataaaaaggg
      661 gtacatcctg tccaatttcc acgttgtcaa gcaggccagc gagatcacgg tcgtcctgat
      721 cgacgggagg gagttcaagg ctacggttgt cggaacggac agtgctaccg atatcgcgct
      781 cctcaagatc gatccctccg gcgagatacc cgttgttgct cttggtgact cggacaagct
      841 cgaggtcggt gactggacga ttgccatcgg gaaccccttc aatctcccgg ggacctttac
      901 cgtcggcgtc gtgagcgcaa aatcccgcgg ggatcagatc ggtgccccct accagaattt
      961 catccagacg gataccgcca tcaaccccgg gaattccggg ggaccgcttg tcaatatcag
     1021 cggtgaggtc gtcggtatca ataccatgat ctatacccgg acaggcggtt ccctcggaat
     1081 cgggtttgcg attccggtca atattgccaa aaatgtcgtg tcctctctca tcaaggacgg
     1141 gaaatttgaa cgcggatata tcgggatcta tcccggtccg atcgatgacg ggatgcggac
     1201 tgcgctcaag cttcccaagg agagcggggt tatcgtcaat tccgtgattg cggacggccc
     1261 agcagccagg gccggaatga aggacgggga cgtgattctt gcgatcgacg gccaggatat
     1321 catatcggta ccccaactca tgcggattgt ggccgaggtt ccggccggaa aaaccgttcc
     1381 gtttgtgatc caacgccaat gacagaagat gacactgcag atcactgtcg tcaaacgacc
     1441 ggatgacgag aagctttccc acaaggaaga tactccccag agtgagactg ccgcctgact
     1501 cgggatgcag attgcaccgg tctcgtcgat caacccgctg caactgcgtc gcctcgggat
     1561 cgatccgaac gagactggcg tggtcattac cggattcagc gagaacgcac ccgagagtga
     1621 actggcaccc ggtgatctta tcaaggaaat aaactacgtt gccatcaatt ccatcgatga
     1681 ctacaggcgc tttgtggaag caaacaaagg aaaaaagagc ttcatcttca aggtcaaacg
     1741 ccaggggcag atgctctggg tcgggatgaa tgcctgacgg gatttcccgt tgtaccgttg
     1801 aaccatgacg caaataacgg tgacctgaca cggttgccgt tgtttgcatg gtacggctct
     1861 cctgttcggg acaaggcatt tatctgttga caatcgattt tgcgccggta tagactgggc
     1921 atgacgcgca gaggcgagtt tccaaccggg caggtatcat ggctgagaca ccgacttttc
     1981 agagacaaaa cccctatgtg gcactcccga cgcgcatgtt tttgacgacc gaagggttac
     2041 gcctttttgc cgccaagccg gaccagttga agcgggtcaa aaaccggatg ggaatcgtca
     2101 aggaagcgat cgaaagcgac cgctataatg cacttctggt tcaaaagctt gtcctcaact
     2161 cctgcatcga agaggtctac gtccggcttc ccaatctcct gcagtaccgg caggatctga
     2221 tcagtaccaa caacctgatt ctgtatgcca ttctctacaa aaaactcagt ccttccctgg
     2281 cggatatgct ttgtcagtca cctgttgtcc aggcctacaa ccggaagaat ccgaaatatg
     2341 ccatcgttgg cctgaacatg atcagtcgtc aaaaaatcga cgctatccgc atggaaaagc
     2401 cccaactttt tgaggccatg gagagcgaga tcatggaaga ggtcaaggcg agaatcaaac
     2461 gccagccgat ttccgatgaa gacaaacaga tccgcgaacg gagcctcgaa aaattcattg
     2521 cctggatcga caagcggatc tggtacatct atctcatcat ctaccagacg cagttgaaac
     2581 gggagctcgt gcgcagtttt accgatatgc tggtcaccta tcttggaagg acccagatag
     2641 cgacccacct ttcgagtctc ttgatggaat ttgtccagaa tgccgagaag gcccattttg
     2701 aacgaatcgt catccgcaag cggtttgccg ccgaaaaaat ggctgattcc ttcatccgtg
     2761 acaggaaaaa cagggaagcc gtgattgccg aagctgttcg tat
//
LOCUS       DAOSHE010000271         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34377_length_2800_cov_2.969035, whole genome shotgun sequence.
ACCESSION   DAOSHE010000271 DAOSHE010000000
VERSION     DAOSHE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34377_length_2800_cov_2.969035"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLD82_07540"
     CDS             complement(<1..314)
                     /locus_tag="PLD82_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65274.1"
                     /translation="MLRSILFFCLSTLAASVSCGDSESISIPVQKKAYLHSMKTLPVP
                     IQFDGLRFDYTLETTSTQMSVFAEPLYDETILINGIKAASRVLSLSNGTNLILVQVSK
                     PGV"
     gene            complement(316..1344)
                     /locus_tag="PLD82_07545"
     CDS             complement(316..1344)
                     /locus_tag="PLD82_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG65275.1"
                     /translation="MKLLPLLLVFLLAGCSLSDDIDTPSISTLTIMTYNVEDMDTGGR
                     NVTNLKAYSGIASLLKQEQVDIVLFQEIQAGSSRMDQYADGSAASWGDTRLFNEALTA
                     QAYRMPWYGFTSRGGSRRDFLAVWSRYPLASITSIDPDRFLDPCNHVWYTPSRPVLRC
                     LTSVSGQKIWLYTAHLKSNAGGVVEMNAGMRRAQAFHLMSYIMRNHDPERDNVVIAGD
                     MNSMPSDYDGLGNSTMDYLCLRYDNPWNTKNDFIPINLTEIGAVTNVPEQRWDNQAPG
                     TTHPGHVSRTGYPDATFDHIILSPALYRKHYVKNSLRIVQVADGYNGGFSDHFPLVLT
                     LQFTNEGL"
     gene            complement(1460..2644)
                     /locus_tag="PLD82_07550"
     CDS             complement(1460..2644)
                     /locus_tag="PLD82_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOG65276.1"
                     /translation="MKKISIILGLLLMAGLAMAADFQPWVGVRGSYQAVFHTDLLDST
                     AAFEVDRARIGVEGEWIKDIYFQVEADAKNTKKGKGTELKVAFIRWSFLDMDTFKGAV
                     EFGALTTTFARGLSGTEYAFINYDITTVPESYQYGVQVKGNLMNNFVEVIASVSDGEG
                     LSTINTAKGLLYVGRVEITPLGSEMKDLREGNTGKTLASGAPMLSIGLAAALDNKAKT
                     DDLGYTSETYNSSHLLADVTFKMLGFSIFGQFVMNSYDALSDGTYWGGVNKNVKESSG
                     GFVQVGYNLKDVVGFELEPMVKFEMWNDVLNEGYGDVDTKKQDFAVGFNWYAHGHDFK
                     MNVEYRHMLKDENFYFGKPPAEDYFGFRVTHKFGAKLSLASKTESKPASTVNTVDKPV
                     EE"
BASE COUNT          725 a          677 c          721 g          677 t
ORIGIN      
        1 acaccgggct tgctgacctg caccaggatc agatttgttc cgttggacag ggagagtacc
       61 cgggaggcag ccttgatgcc gttgatcagg attgtttcat cgtacagcgg ttcagcaaag
      121 accgacatct gagtgcttgt tgtctcgagc gtatagtcaa accgcagccc atcaaactgg
      181 atcggtacgg gaagggtttt catcgaatgg aggtatgcct ttttttgaac agggattgaa
      241 atgctctcgc tatccccgca cgaaaccgaa gcggcaagag tgctcaggca gaagaagagg
      301 atggatcgca gcatgtcaca gtccttcgtt ggtaaactgg agcgtgagta caagcgggaa
      361 atgatcggaa aatccgccat tgtatccgtc tgcaacctga acaatccgca gggagttttt
      421 gacatagtgc tttctgtaga gtgcaggtga cagaatgatg tggtcaaacg tggcatcggg
      481 ataaccggtc cgggaaacat gtccgggatg ggttgttccg ggagcctgat tgtcccagcg
      541 ctgctcaggg acatttgtga cggcaccgat ttcagtcagg ttgattggaa taaagtcgtt
      601 ttttgtattc cacgggttgt cataccgcag gcagagataa tccatggtgc tgttccccag
      661 tccgtcatag tcggagggca tgctgttcat gtcaccggca atgacgacat tgtcccgttc
      721 gggatcgtga ttccgcatga tgtaggacat gagatggaag gcctgggcac gtctcatgcc
      781 ggcattcatt tccacaacac cacccgcatt ggatttcagg tgtgccgtgt agagccagat
      841 tttctggccg gaaacgcttg tcaaacagcg aagaacgggc cgggacgggg tgtaccagac
      901 atgattgcac ggatcaagaa acctgtcagg atcaatggag gtgatggaag cgagaggata
      961 acggctccag acggccaaaa aatcccgccg ggaaccacca cgtgaggtaa atccatacca
     1021 cggcatccgg taggcctggg cagtcagggc ttcattaaag agacgggtat caccccagga
     1081 ggctgcagag ccatccgcat actgatccat ccgtgaagaa ccggcctgaa tctcctgaaa
     1141 aaggacgata tcaacctgct cctgcttaag gagggaggcg atgcccgaat aggctttgag
     1201 attggtgaca ttgcggccgc cagtatccat gtcttcgaca ttataggtca tgattgtcag
     1261 tgtcgagatg gatggtgtat caatatcgtc actgagactg cacccagcga gcagaaaaac
     1321 cagcaggagc ggaaggagtt tcatgatacc gcctcatatc aacttggata atcagcattg
     1381 tactccgtca ggttgatatt gcaataaaaa aacgcctccc tgacaggagg cgttgtgaac
     1441 aagttacaac aatacagact tattcttcaa ccggtttgtc aacagtgttt acagtgctgg
     1501 cgggctttga ttcggttttg gaagccagag acaacttggc tccgaactta tgggtaacgc
     1561 ggaagccgaa ataatcttct gctggcggct tgccaaagta gaaattctca tccttgagca
     1621 tgtgtcggta ttcgacgttc atcttgaaat catgcccatg tgcgtaccag ttgaagccaa
     1681 cagcaaaatc ctgttttttc gtgtcaacat cgccgtagcc ctcgttaagg acatcattcc
     1741 acatttcgaa cttgaccatg ggctcgagtt cgaagccaac gacgtccttc aggttatatc
     1801 caacctggac aaagccaccg gaggactctt taacgttctt gttgacgcca ccccagtatg
     1861 ttccgtcgga aagtgcatca taggaattca tgacgaattg accaaaaatt gagaaaccaa
     1921 gcatcttgaa tgttacatca gcaagaaggt gtgagctgtt atatgtttct gaagtatatc
     1981 cgagatcatc cgtcttggct ttgttatcaa gagctgcagc caatccgatg gaaagcattg
     2041 gggcgccgga tgcgagtgtt ttaccggtat ttccctctcg aagatctttc atctcgcttc
     2101 caagaggtgt gatttccaca cgtccaacat agagcagacc cttggcagtg ttgattgtgg
     2161 acaagccttc accatcagaa accgaagcga tcacttcgac aaaattgttc atgagattcc
     2221 ctttgacctg aacaccgtac tgatacgact cgggaactgt agtgatatca taattgataa
     2281 acgcgtattc agttccagag agacctcggg caaatgttgt tgtcaaagca ccaaactcaa
     2341 cagcaccctt gaacgtgtcc atatcaagga acgaccagcg gataaaggca accttgagct
     2401 cagttccctt gccctttttt gtgtttttgg catccgcctc aacctggaag taaatgtcct
     2461 tgatccactc cccttcgaca ccgatccggg cgcgatcaac ttcaaaagcg gcggttgaat
     2521 cgagaaggtc ggtatgaaaa acagcctgat agctgccgcg cacgcccacc cagggctgga
     2581 aatcggctgc catggcgagg cccgccatga ggagcagacc aaggatgatc gaaatctttt
     2641 tcattgaatt cctccgaatt aatgtgtgca attttcgtat gtatgttttt caggtcaaac
     2701 ctgctgtaca agagtatccc cccggaaaaa aacttccggg gtgtaaaccg ggttacggaa
     2761 cgatcatgta ctccttgcct ttcatcatga tcgaggcacc
//
LOCUS       DAOSHE010000272         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34388_length_2799_cov_3.721210, whole genome shotgun sequence.
ACCESSION   DAOSHE010000272 DAOSHE010000000
VERSION     DAOSHE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34388_length_2799_cov_3.721210"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLD82_07555"
     CDS             complement(<1..1184)
                     /locus_tag="PLD82_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG65277.1"
                     /translation="MHTIKSRIIILMTVSAVFFALLYIYQEAKGDMVLAVKDGEMATL
                     FKHSVEQLDRQTTFMKQSALRLAAGGSALHAARSGMSPDTAHAVVKRFIMESMRSLPE
                     ALGGGLWFEPYAFFPDRRWYGPYAYHEDGELVYTQEYDTPEYDYHQRDWYTVALPIGW
                     DRGQVRDGRVWWSAPYEDRVGKERYLITTSAVIQDAEGRYIGVTSTDWGVRGMADFIT
                     SLGTAKGVETWLIHQPSGRVLLSTGRGEHSLPILSDWRKTGLASLVGEPEAGKTIIRN
                     GLKISGKTWHSWSGLSRDGFLLVQLVPDEDRLAEIRSVNTGGRILFLTLCAIVSLIVW
                     LGTRTFKREMDRMLAGLERITLETPESWSTQVWQYQEIRQIAHALTRMAERIHHLSDE
                     RLA"
     gene            complement(1259..2593)
                     /gene="rseP"
                     /locus_tag="PLD82_07560"
     CDS             complement(1259..2593)
                     /gene="rseP"
                     /locus_tag="PLD82_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG65278.1"
                     /translation="MDTVLSIVYGLITLGILVFVHELGHFLAGKAVGIRVTAFSIGFG
                     RGIVSFERGGTLYKIGWIPIGGYCRFAGEGEDLGDDKKGEPDEFYERPAWARLITVSA
                     GVVFNFVLAILIFIVLGLTGYSYTSPDNRITVIDQTIDPKAGVWPAATAGLRTGDKIR
                     SVDGIATPDFRKLQEEIAVRPDRTIRLEILRSTNAGQIAVDSIIKDTGMGYIGILPYY
                     GTTVSGVESGTPAKKLGLLPGDRILAWNSLPVQSLHELKARIAETGAREVEIRWERKG
                     KILQGRASAIQRDGAWVLGFTPTPPVLQEYSLDGLSIGESLAGAFSLFGQNIGRIVDG
                     IALLFRKEVDAGKAVAGPLRIVHFSGQVMKESTTAGYLMFLAMISIALGFFNLLPIPA
                     ADGGHVVLTLIEMIRRKRFSFAVLRRIQMTGIIILMSLFIFVMFFDIMNIIR"
     gene            complement(2636..>2799)
                     /locus_tag="PLD82_07565"
     CDS             complement(2636..>2799)
                     /locus_tag="PLD82_07565"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG65279.1"
                     /translation="VGDLAESVLKRSLGTKDSGSLVPGHGGLLDVFDAQMVVSPLVLG
                     LTILLAGAG"
BASE COUNT          678 a          849 c          717 g          555 t
ORIGIN      
        1 gccagtctct cgtcggaaag atgatgaatc cgttctgcca tccgggtcaa ggcatgggcg
       61 atctgccgga tctcctgata ctgccaaacc tgggtcgacc agctctccgg tgtctcgaga
      121 gtaatccgtt caagaccggc caacatccgg tccatctcgc gcttgaatgt ccgtgttcca
      181 agccagacaa tcagggaaac aatggcgcag agcgtcagaa agaggatgcg cccgcccgta
      241 ttgaccgacc gaatctccgc cagacggtcc tcgtccggaa caagctggac caacaaaaac
      301 ccgtcccgac tcaatccgct ccaggagtgc caggtttttc cggaaatctt caatccgttc
      361 cggataatgg tcttcccagc ctcgggttcg cccaccaggg aggccagccc ggtttttctc
      421 caatcggaca gaatcggaag agagtgctct cctcttccgg tactcagcag gacccggccc
      481 gagggctggt gaatcagcca tgtctcgaca cccttggctg tccccagact tgtgatgaaa
      541 tcggccattc cgcgaacacc ccagtccgtc gaggtgacac cgatataccg tccctccgca
      601 tcctggatca cggcactggt cgtgatcaag tagcgttctt ttccaacccg gtcttcatag
      661 ggtgcactcc accagactct gccgtcacgg acctgaccgc gatcccatcc gatgggcagg
      721 gctacggtgt accagtccct ctggtgataa tcatattcgg gagtatcgta ctcttgggta
      781 tagacgagtt ccccgtcctc atgataggcg tagggcccgt accagcgccg atccgggaag
      841 aaagcgtacg gctcgaacca aagcccgcca cccagtgcct cgggcaagga acgcatactc
      901 tccatgataa accgtttgac aacggcatga gccgtatcgg gtgacatccc gctccgcgca
      961 gcatgcaagg ccgatccacc cgcagcgaga cggagtgcac tctgtttcat gaaggtggtt
     1021 tgcctgtcaa gctgctcaac ggagtgcttg aagagcgttg ccatctcgcc gtccttgacg
     1081 gcaagcacca tatctccctt ggcttcctgg tagatataga gcaaggcaaa aaaaacggca
     1141 gaaaccgtca tcaggataat gatgcggctc ttgatggtat gcatcactcc tcctgttcca
     1201 atccccgtaa acgagtatac acgtttgcgg ggattgcaca agagtgaaag gaaaatcact
     1261 atctgataat attcataata tcaaaaaaca tgacaaagat aaaaagcgac atgaggatga
     1321 tgatcccggt catttggatc cgccggagaa ccgcgaaaga gaaccgcttg cgccggatca
     1381 tctcgatcaa ggtcaggacc acatgtccac cgtcggccgc cggaatcggg agaaggttga
     1441 aaaacccgag cgctatggaa atcatcgcca ggaacatcag atagccagcg gtcgtgctct
     1501 ccttcatgac ctgcccggaa aaatgcacaa tcctcaaggg tccagccacg gccttgcctg
     1561 catcaacttc cttgcgaaag aggagggcaa tcccgtcaac aatcctgccg atattttgcc
     1621 cgaacaggga gaatgctccg gccaacgact cgccaatgga gagaccgtcc aatgagtact
     1681 cctgcagcac gggtggtgtg ggtgtgaacc cgaggaccca tgccccgtca cgctggatgg
     1741 cagaggcgcg tccctggagg atcttcccct tgcgttccca acggatctcg acctcacggg
     1801 caccggtttc ggctatcctg gccttgagtt catgcaggga ttggacagga agggaattcc
     1861 atgcaagaat ccggtccccg ggaagcaggc ccagcttctt ggccggtgtg cccgattcga
     1921 caccactgac agttgttccg taatatggaa ggataccgat atatcccatc ccggtatcct
     1981 tgatgatgga gtcgacagca atttgtcccg catttgtaga acggagaatc tccaaccgga
     2041 tcgtccggtc cggtcggacg gcgatctcct cctgcaactt cctgaaatca ggagtggcaa
     2101 taccatcaac ggatcggatt ttatccccgg tcctgagacc ggcagttgct gcaggccaga
     2161 cacccgcctt gggatcaatc gtctggtcga tcaccgtgat cctgttgtcc ggagaggtat
     2221 agctgtaccc ggtcaatccg aggacaatga aaatcaggat cgcaagaacg aagttgaaaa
     2281 cgacaccggc gctgaccgtg atcagtctgg cccaggccgg gcgttcgtag aactcgtccg
     2341 gttccccttt tttgtcgtca ccaagatcct cgccctcacc tgcgaaacgg caatagccgc
     2401 cgatcggtat ccagccgatc ttgtagaggg taccaccccg ttcaaaggaa acgatccccc
     2461 tcccgaatcc gatggagaag gccgtcaccc ggatcccgac ggccttaccc gccagaaagt
     2521 ggccaagctc gtgtacaaag acgagtatcc ccagcgtgat caaaccatac actatcgaaa
     2581 gtactgtatc catcgcccca tccaatcctc atcccgcaaa gtacgccaat gtgcttcaac
     2641 ccgctccggc caggagaatc gtgagtccca acacaagggg agaaacaacc atctgggcat
     2701 caaaaacatc cagaagacca ccatgaccgg gaacaagact ccccgaatcc ttggttccga
     2761 gcgagcgttt caaaacactc tcggccaagt caccgacct
//
LOCUS       DAOSHE010000273         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34392_length_2799_cov_3.285350, whole genome shotgun sequence.
ACCESSION   DAOSHE010000273 DAOSHE010000000
VERSION     DAOSHE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34392_length_2799_cov_3.285350"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="PLD82_07570"
     CDS             <1..1787
                     /locus_tag="PLD82_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65280.1"
                     /translation="NLVETLADVLKKQYSESAGVTLGGRLPAGFTWKWLASDSLFISD
                     SSRQTNGVAGSWYTNAATTATATWNRKESLSLIWGADNFLGINRSQAQVSLTTFAERE
                     RYDSTRSRDGMRDLIRTYDMMGPMEAWAERTASLLSGMGRIGDSVGGTNIGAIGEGMN
                     YTAAFRNLGFLSLTASFSRSARDANFVRMLLPAGQTNYVHANNDTTTAKSQYGLVLAF
                     PEFPLRSLSLDLSRDLSYSQTAGQMGESQIWRELDGVFMSQPFSGSDVLGLGSRHAAL
                     DDVSRLKDEGNSYLSISHRIALSLRLDYGETLFADFLPADWQLTETQNTARNLMSYVQ
                     NRQRSLSFNKNFPLKKLGFWLFDEASSPGKSVQDLALKMQIGRSWDYNTKQVSDTLAA
                     SLGFNVNWNRSTTMALAWEVTRSRTEQFLVAGDAGYEYMGLGADSGDGRGDSFGDLEE
                     LAGGKAPAITEVPGLTKWDHGIRFDIGFQTKHSGTLEIFRLKIPIDTEWKHMNEVLIR
                     FSTSEYDRNREGRYNFSEIFERTFYLRLKHSIDYDFTSNWNGVLHLAFVFERWRLVLP
                     SSDGFALNEDDFGLSFGFEVGLKMQIRF"
     gene            1861..1933
                     /locus_tag="PLD82_07575"
     tRNA            1861..1933
                     /locus_tag="PLD82_07575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1894..1896,aa:Thr,seq:ggt)
     gene            1999..2160
                     /gene="rpmG"
                     /locus_tag="PLD82_07580"
     CDS             1999..2160
                     /gene="rpmG"
                     /locus_tag="PLD82_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG65281.1"
                     /translation="MAKSVREKIQLACQECKRRNYSLTKNKKLHSEKLELSKFCPWDR
                     KHTVHKEVK"
     gene            2189..2261
                     /locus_tag="PLD82_07585"
     tRNA            2189..2261
                     /locus_tag="PLD82_07585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Trp,seq:cca)
     gene            2401..2574
                     /gene="secE"
                     /locus_tag="PLD82_07590"
     CDS             2401..2574
                     /gene="secE"
                     /locus_tag="PLD82_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG65282.1"
                     /translation="MFEFFRESKAELKKVEWPEKQEVVNSTVVVLVSLVVVSLGLGGM
                     DALMTFLIKLIGE"
     gene            2576..>2799
                     /locus_tag="PLD82_07595"
     CDS             2576..>2799
                     /locus_tag="PLD82_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOG65283.1"
                     /translation="MARHWYILHTYSGYENKVKQGIDRLTRLELADVVHDVKIPVESV
                     IEMRSGKKRETKKKFFPGYILVEMDIPEDE"
BASE COUNT          613 a          698 c          834 g          654 t
ORIGIN      
        1 ccaatcttgt cgagacgctg gccgatgttc tcaaaaaaca gtacagcgag agtgccggcg
       61 tcacgcttgg cgggagactt ccggccgggt tcacatggaa gtggttggcc agtgattccc
      121 tctttatctc ggattcgtca cggcagacaa acggggtggc cgggtcatgg tataccaatg
      181 ccgccacgac tgcgacggcc acctggaacc gaaaggagag tctcagcctg atctggggtg
      241 ccgacaattt tctcgggatc aatcgttccc aggcccaggt ctcgctcacg acctttgccg
      301 agcgggagcg ctatgattcg acccgttcgc gggacgggat gcgggacctg atccgtacct
      361 atgacatgat gggaccgatg gaagcctggg cagagagaac ggcctccctg ctctccggaa
      421 tgggccggat cggggatagc gtcgggggta ccaatatcgg ggcgatcggg gaagggatga
      481 attatacggc agccttcagg aatctcggat tcctctcgct gacggcatct ttttcacgga
      541 gcgcccggga tgccaatttt gtccggatgc tcttgccggc cgggcagaca aattatgtcc
      601 atgccaacaa cgacacgaca acggccaaga gccagtacgg gcttgtcctc gcgtttccgg
      661 agtttccctt gcggagtctc tccctggatc tttcccggga tctctcgtat tcccagaccg
      721 ccgggcagat gggagaatcg cagatctggc gcgaactgga tggtgtcttc atgagccagc
      781 cattttccgg aagcgatgtc ctcgggttgg ggagccggca tgcggcgctg gacgatgttt
      841 cgcgactgaa agacgaggga aactcgtatc tctcgatttc ccatcggatt gcgctttccc
      901 tgcgactcga ttatggggag acgctcttcg ccgattttct gcctgccgac tggcagttga
      961 cggagacgca gaatacggcc cgcaacctga tgagttatgt gcaaaacagg caacgctccc
     1021 tctcgttcaa caagaacttt ccgttgaaaa aactcggatt ctggctgttt gatgaagcat
     1081 cctctcctgg aaagagcgtt caggaccttg cgctcaagat gcagatcggc cggtcatggg
     1141 attacaacac aaagcaggtc agtgacacac tcgcggcatc gcttgggttc aatgtcaact
     1201 ggaacaggtc caccacgatg gccctcgcgt gggaagtgac gcggagcagg acggagcagt
     1261 ttctggtggc gggtgatgcc ggctacgagt atatgggcct tggagccgac agcggggacg
     1321 ggcgggggga ttccttcggt gatctcgaag aactggcggg cgggaaggcc ccggccatca
     1381 ccgaggttcc cggtctgacc aagtgggatc acgggatccg cttcgatatc gggttccaga
     1441 caaagcactc cggaacactg gagattttcc gcctcaagat tccgattgat accgaatgga
     1501 agcacatgaa cgaggtcctg atccggttct cgacgagcga gtatgaccgg aaccgggagg
     1561 ggcggtacaa cttcagcgaa atttttgaac ggacattcta tctgcgtctc aagcactcga
     1621 tcgattatga ctttaccagt aactggaatg gggtcttgca cctggccttt gttttcgagc
     1681 gctggcggct tgtcctgccg tcttcggacg gttttgcact gaacgaggat gatttcgggc
     1741 tctcgttcgg gtttgaggtt gggctcaaga tgcagatccg gttctgaggg aagaaaaaag
     1801 tttgaaaaat ttgccgtatc gcccgggctt gactatattt ttgcagcgtt acctgcgcat
     1861 gccctcgtag ctcagtcggt agagcacttg catggtaagc aagtggtcac cagttcaatt
     1921 ctggtcgagg gctcattttt ttgagtatcg tgaccgtctc cggttttctg gcggtcaaag
     1981 attcagggaa ggtatccaat ggcaaagtca gtacgcgaga aaatccagct tgcctgtcag
     2041 gaatgcaagc ggcggaatta ctctctcaca aagaacaaga agttgcattc cgaaaaactt
     2101 gagctctcca agttttgccc gtgggatcgc aaacacacgg tacacaaaga agtgaaataa
     2161 tcccggacac ttttgtggcc gggtgtctgg gacagtagct ccaatggtag agcgggtgac
     2221 tccaaatcac taggctgggg gttcgagtcc tccctgtccc gtttcttttt ttatgggcat
     2281 atctgtttcc ttgtgagtag cttactgggg aacagtctcc agcccggtgg ttgcgatctt
     2341 gtcgatgcag ccgaactggt tggtgcagta ccatcatgga aggtgataac cgtgaaacgc
     2401 atgttcgaat ttttccggga gtccaaggca gagctgaaaa aagtcgagtg gcccgaaaag
     2461 caggaagtgg tcaattcgac ggttgttgtc cttgtttctc tcgtcgtcgt ctcgctcggg
     2521 cttggcggca tggatgccct gatgacgttt ttgatcaagc tgattggtga atgatatggc
     2581 gcggcattgg tacatcctcc atacctattc ggggtatgag aacaaggtca agcagggtat
     2641 tgaccgcttg actcgtcttg agctggccga cgttgtgcac gatgtgaaaa tccccgtgga
     2701 gagcgtgatc gagatgcgct cgggaaaaaa gcgggaaaca aaaaagaagt tttttcccgg
     2761 atatattctt gtcgagatgg atatccccga agacgagca
//
LOCUS       DAOSHE010000274         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34465_length_2794_cov_4.297189, whole genome shotgun sequence.
ACCESSION   DAOSHE010000274 DAOSHE010000000
VERSION     DAOSHE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34465_length_2794_cov_4.297189"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLD82_07600"
     CDS             <1..268
                     /locus_tag="PLD82_07600"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65284.1"
                     /translation="LAARIQARQLKISFDPHVRSMLAREGYDPQFGARPLARLIQKKI
                     VAPLSVLLLEESIAEGSSIDCRLEGEAVRFAIRSPEETAETPAP"
     gene            602..1864
                     /locus_tag="PLD82_07605"
     CDS             602..1864
                     /locus_tag="PLD82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG65285.1"
                     /translation="MQTDPSQLSLAAYKQGSYILVEDKVDNPFFYIVKSGQVRVQTKS
                     DLVTSDVVKEDSDSVLNPGDFFGVISAMSGHPSIETVIALTDVVLIVVKRDQFGMLIQ
                     KNGPIAMKIIRSFSRKLRYYDTALTRLSFSNSVEEDPMHLLSLGEYYFKQKSWVTAAY
                     AYRKYLEYNPGSTHVMQARQRLAQLAPFLKAQPQQPQQQAQGAGFARVYEEGDVIFLE
                     HEIGNELYIIQEGGVKIKKVVANNEITLAILQKGDIFGEMAILDDKPRSATAVAYGRA
                     VLLAVNKTNFQGMVQQQPQLATKIITLLSERIWTSYRQFANAVIDDPAGKIFDMLLTQ
                     VVKNHIPLDKGTAHTFDFGANELLGMIGLSPDQGKTVLKDFFANNKKFLLVNNKMHVV
                     DLEELHKQANYYKNQWLRKRKMEESRGI"
     gene            complement(2339..2776)
                     /locus_tag="PLD82_07610"
     CDS             complement(2339..2776)
                     /locus_tag="PLD82_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG65286.1"
                     /translation="MEEGIVPGGGMSLLSCAKSLESFKINDEEEAIGIQIVRRALEAP
                     IKQIAINAGIDGAVVAERAKTEKPGFGFNAATNTWENLIDAGIVDSAKVARVALQKAA
                     SIAALMLTTETLVSDKPEPKKASMGGDPGMGGMGGMGGMGMGY"
BASE COUNT          646 a          814 c          722 g          612 t
ORIGIN      
        1 actggctgcc aggattcagg cccgccagct caagatcagt tttgatcctc acgtccgcag
       61 catgctggct cgggagggct acgatcccca gttcggggcc agacccttgg cacgcctcat
      121 ccagaaaaaa atcgtcgcac cactctctgt tcttcttctt gaagagagca tcgcggaagg
      181 aagcagtatc gattgccgac tggaggggga ggccgtccgc ttcgccatcc ggtcaccgga
      241 ggagacagca gagacgccag ccccctgatc ccggtgtcgg aacgtgggac agaagaacgg
      301 gcacactcca gggagcatca cgacccggga ccggccaggt cccgggttct tgttcttcaa
      361 gcagggactc atccccgttc accgcgatga ccctgctttt gagagaatct ctccacaacc
      421 gctccgtgaa ggtgtggatg ccaccactga atgcaagtca tattgcagaa ttcgcccctc
      481 cctgccgata cataaaagaa gactgcccag tttccatctt ccggtacagg gcaattgaca
      541 cagaacccgg acgggcgtat tctggtacca ggccaaaccg gttggtcatg acggagaaga
      601 catgcagact gatccttccc agctttcact ggcggcctac aagcaggggt cgtacattct
      661 cgtcgaagac aaggtcgata accccttctt ttatattgtc aaatccgggc aggttcgggt
      721 ccagaccaaa tccgatctcg tcacatcgga tgtcgtcaag gaagacagcg acagtgtttt
      781 gaatcctggc gatttttttg gcgtcatctc ggccatgagt ggccacccca gcatcgaaac
      841 agtgatcgct ttgactgatg tcgttttgat cgtcgtcaag cgcgaccaat tcgggatgct
      901 gatccagaaa aacgggccga ttgccatgaa aatcatccgc tcgttttcac gaaaactccg
      961 ctattacgat accgccctga ccagactttc attttccaac tcggtcgaag aggatccgat
     1021 gcacctgctc tccctggggg agtactactt caagcagaag agctgggtga cagcggccta
     1081 tgcctatcgg aagtatctcg aatacaatcc gggcagcacc catgtcatgc aggcccgcca
     1141 acgcctggcc cagctcgccc cctttctcaa ggcacaaccc cagcagcccc agcaacaggc
     1201 acagggagcc ggttttgccc gtgtctacga ggagggcgat gtcatcttcc tcgaacacga
     1261 gatcgggaat gaactctaca tcatccagga gggtggcgtc aaaatcaaaa aggtggtcgc
     1321 caataacgag atcaccctcg cgattttaca aaagggtgac atcttcgggg aaatggcgat
     1381 cctggatgac aagccacgtt cggcaacggc tgttgcctac gggcgagccg ttttgctcgc
     1441 cgtcaacaaa acgaatttcc aggggatggt ccagcaacag ccgcaactcg cgaccaagat
     1501 catcaccctc ctctccgaac ggatctggac ctcctaccgc cagttcgcca acgccgtgat
     1561 cgatgatccg gctggcaaga ttttcgatat gctcctcacc caggtcgtca agaatcacat
     1621 cccgttggac aagggaacag cgcatacctt cgactttggt gccaatgagc tcctcgggat
     1681 gatcggactc tccccggatc agggaaagac ggtattgaag gacttttttg ccaacaacaa
     1741 gaaatttctc ctggtgaaca acaagatgca tgttgttgac ctcgaggaat tgcacaaaca
     1801 ggccaattac tacaagaacc aatggctccg caaacgcaaa atggaagagt cccgcggaat
     1861 ctgagacgat caacttgtcc cggtggctgt ccatcggccc gttgaacgat gtccacgccc
     1921 ggaattgctt gagcctgcat tgcaatccgg gatagcccga ttgcctgaat tgtacgaaga
     1981 ttccatactg tactttcgcc acgagttttc aaaaccgcct gtgtttttga catacaccgt
     2041 tctttggggc gtccaacaga ggagtactcc gggagtgttt cgtgtgtcaa ttgtacacaa
     2101 tgctcattat ctctcttgca accatccgta gattgcctgg ttgtggtcaa aatgacccac
     2161 tttgtctctt tttgcaatcc cggattgcac aatagccctt tgaggcgaat cgatcctgca
     2221 atcgagcagg aggcgggctt gcgcccgccg tcctcgaatg tacgccctgc cgggcgtata
     2281 gcacacaaaa aaccggcctt gtggccggtt ttttgaacat cagatgacag atcttggatc
     2341 agtagcccat tcccatgcca cccatgccgc ccatcccgcc catgccgggg tcaccgccca
     2401 tggaagcctt tttgggctcg ggtttgtcgg aaacaagggt ctctgtggtc aacatcaggg
     2461 cagctatgga agctgccttc tgcagggcaa cccgggcgac tttggccgaa tccacgatac
     2521 cggcgtcaat caggttttcc catgtattgg tggctgcatt gaaaccaaaa cccggttttt
     2581 cggtcttggc acgctcggca acaacggctc cgtcaatccc ggcattgatg gcaatctgct
     2641 tgatcggagc ttcgagtgca cgccggacga tctggatacc gatcgcctct tcctcatcgt
     2701 tgatcttgaa ggactcgagc gacttggcac aggagaggag ggacattcca ccaccgggga
     2761 cgatgccctc ttcgatggca gcgcgggtcc ttca
//
LOCUS       DAOSHE010000275         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34664_length_2783_cov_4.013196, whole genome shotgun sequence.
ACCESSION   DAOSHE010000275 DAOSHE010000000
VERSION     DAOSHE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34664_length_2783_cov_4.013196"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(494..799)
                     /locus_tag="PLD82_07615"
     CDS             complement(494..799)
                     /locus_tag="PLD82_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOG65287.1"
                     /translation="MIKHIVLWKLKDEAAGRSKKENALRIKTLLEDLNGRIDGLIRLE
                     IGLDIGADPAAVDVALYSEFVDRAALDFYQTHPMHEAVKPLVLAVRESRTVVDFETD"
     gene            complement(888..1559)
                     /locus_tag="PLD82_07620"
     CDS             complement(888..1559)
                     /locus_tag="PLD82_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65288.1"
                     /translation="MNAITRLALVCLALLVMLPMGLPAQSGPGWELIRHPNNIDKIRE
                     QVTEYANQGKTPMGMSIVGDQVYILYITGNKFKLDRWEINWYDSVDELSTGVTAKMNA
                     GFMPVGLHYSGSQFFIIYIKTAFKATHWIIVPSERTLESLQQEIADLTSDYYVPMDLA
                     ARGDNFYTLLVRIPTTTIKQWHIVTYQANNTAITSGINGMVNQGWNPWGMDFDGNAVR
                     ILFVK"
     gene            2018..>2783
                     /locus_tag="PLD82_07625"
     CDS             2018..>2783
                     /locus_tag="PLD82_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG65289.1"
                     /translation="MKMRVCSLCVGLAMLGLVVTVHADRDVKAHETGSATMVLVPGGT
                     FRMGNVRADNEGSDSERPVHQVTLSPYWIGKYEVTYPLWVRVRDWAKANGYEFENEGR
                     EGYGQSQTTATPQANSIDEDAPAALASNTPTFSEQPVVMINWYDMVKWCNAYSQMQGL
                     TPCYYTGSAFRTVYKTGRKDLTPQLVNWNANGYRLPTEAEWELAAKGGAAGAANPFTY
                     AGGDNPDEVAWYKKNSGVATHFVGTKRPNQLGIHDMS"
BASE COUNT          601 a          731 c          854 g          597 t
ORIGIN      
        1 agggcctcgg cgacggccag ggtcaggatc gtgtcgtcag tgaaccgcgc ctgcggcgga
       61 aagagcggaa agtccgcgtc atatgtcgga tggaattcat aggccgaacc gatgatgtct
      121 ccggcgattg cgccaatcat tggttccctc ccgggctgag gccgtctttg ccgcccgatc
      181 ccgacatatg gtatacaggc acaggccggg gggctgcaag aaaaaaatct tcctgcaaaa
      241 ttctttctgc cggagattcg cgaaccctgc agaccgctgc caagcgccgt ccgtctcgac
      301 ctcgaaagac gtcgttgacc tgaaatgggt cgagaagtgg aacggcatcc tgccacagat
      361 gatgggcggc aacgccatga tcccgatggt caacttcggg aaataattat gggaagctgg
      421 ggctgtcgcc cccggacccc cgtttaaaga cagaacgaac acctgaccgg gagtgtgtcc
      481 tctgcagccc ggctcagtct gtctcgaaat cgacgacggt gcgcgactcg cggaccgcga
      541 gcaccaaggg tttgaccgct tcatgcatcg ggtgggtctg gtagaaatcc aaggccgccc
      601 ggtcgacgaa ctcgctgtag agtgccacgt caacggcggc cggatcggcg ccgatgtcga
      661 gtccgatctc aaggcgaatc agcccgtcga tgcgtccgtt caagtcttcg agcagggtct
      721 tgatgcgcag ggcgttctct tttttggaac gccccgccgc ctcatctttg agcttccaga
      781 gaacgatgtg tttgatcaca atgggtacct gtcagatcag agtatgcaac agggctgcgt
      841 actctgatca tgactgcttg cgcacaagat gtcaagtgga tccggactta tttgacaaac
      901 aggatacgga cggcattgcc gtcaaaatcc attccccagg ggttccagcc ctggttgacc
      961 atgccgttga tgccggatgt aatggcggtg ttattggcct gataggtcac aatgtgccat
     1021 tgcttgatgg ttgtggtcgg tatgcgcacc agaagggtgt agaaattgtc gccccgggca
     1081 gcaaggtcca tggggacgta gtagtcagag gtcagatcgg caatctcctg ctggaggctt
     1141 tcgagcgttc gctcgctggg gacaatgatc cagtgggtgg ccttgaaagc ggtcttgatg
     1201 tagatgatga aaaactggct tccgctgtaa tgcaggccga cgggcatgaa gccggcgttc
     1261 atctttgcgg taactccggt gctcagttcg tcaacgctgt cgtaccagtt gatttcccag
     1321 cggtcgagct tgaatttgtt gccggtgatg tagaggatgt agacttgatc cccgacgatc
     1381 gacatgccca tcggggtttt cccctggtta gcgtactcgg tcacttgttc gcggatcttg
     1441 tcgatgttgt tggggtggcg aatcagttcc cagccgggac cgctctgggc cgggaggccc
     1501 atggggagca tcacgagcag ggcaagacag acaagggcaa ggcgggttat ggcgttcatg
     1561 actttctcct ttatggtgcg cgaacctgat tttggtgtca gtttcaacct ccagaaacac
     1621 gcaccgcact gaaaatacac caccccgcct gtttcttcaa aaaaaattgc ctacggtgtg
     1681 gtctgatgtt caatatccct gcgggatcga caccgggatt gtgtataccg tggttgattc
     1741 gcgaaccggt cctgcgaata cgatgcatgc acgacggtgg ggtgggagac acaagatttt
     1801 gcgtctctac ggatgccgtg gtgatgtgcc gaaatgaccc gaatatgcac gtatgcgaca
     1861 acggctccca ttattgtgtg gcgtgttttt gtaggggtcg gtttgaaacc gacccctacg
     1921 gcggatgccg tggtattgag gcgcgaaaat ccgggcagga tgacaaatgc gtgtagaaaa
     1981 cgtatatata cagtagaaga aaatccaagg agtgtccatg aaaatgagag tgtgcagtct
     2041 gtgtgttggc ctggccatgc tggggctggt cgtgacggtc catgcggacc gggatgtcaa
     2101 ggcacacgag acggggagtg ctaccatggt ccttgtccct ggcgggacct tccgtatggg
     2161 caatgttcgg gcggacaatg aaggaagtga ttcagaacgg cccgtccatc aggtgacgct
     2221 ctcgccctac tggatcggca agtacgaggt gacgtacccc ttgtgggtac gtgtgcgcga
     2281 ctgggccaag gccaatggct acgagttcga aaacgaaggc cgggaaggct atggtcagtc
     2341 acagacgaca gcgacccctc aggccaatag catcgacgag gatgcgccgg cggctttggc
     2401 ctccaacacg cctacctttt cggaacaacc agttgtcatg atcaactggt acgacatggt
     2461 caagtggtgc aatgcctatt cacagatgca ggggctgacc ccctgctact ataccggcag
     2521 tgcctttcgg accgtctaca agacgggccg caaagacctg acaccccagc tcgttaactg
     2581 gaacgccaac ggctatcgcc tgccaaccga ggccgagtgg gagttggccg ccaagggtgg
     2641 ggcggccggg gctgccaacc ccttcacgta cgccggcggg gacaatccgg atgaggtggc
     2701 ctggtacaaa aaaaacagtg gcgttgctac gcatttcgtt ggtaccaagc gtcccaacca
     2761 gttggggatc cacgacatga gcg
//
LOCUS       DAOSHE010000276         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34665_length_2783_cov_3.907625, whole genome shotgun sequence.
ACCESSION   DAOSHE010000276 DAOSHE010000000
VERSION     DAOSHE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34665_length_2783_cov_3.907625"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLD82_07630"
     CDS             complement(<1..274)
                     /locus_tag="PLD82_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG65290.1"
                     /translation="METNFVGTAFDPAQSVAYAEGSVVSKTILDKKEAGNLTLFSFDA
                     GQGLSEHTSPYDAVVHILDGEAEITIDGDAQTVRAGEMLIMPANKPH"
     gene            complement(292..858)
                     /locus_tag="PLD82_07635"
     CDS             complement(292..858)
                     /locus_tag="PLD82_07635"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOG65291.1"
                     /translation="MFPKNTLLRYISIVMFVAVILPLHGWQLGGKNMVLAGTGEREKF
                     FVTIYFASLYVPANLKGRSGEAIVAADEPMSVILKVDSSKLTRDRFMSATREGFQDVA
                     ALGYPAPERERFLAFFTGINIQVGDLIYLTYVPGSGLTASYQVKGTQSQRTLGTIPGL
                     AFKKALFAIWLGPDPVQSGLKRGMLGQN"
     gene            complement(1123..1638)
                     /locus_tag="PLD82_07640"
     CDS             complement(1123..1638)
                     /locus_tag="PLD82_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG65292.1"
                     /translation="MQDGNLDQLRSRRSVRRFLPKSVSRETVTSLLDVAMNAPSAGNE
                     QAWQFVVLDDRTLLEKYAALNPNVAFVSDAPVAILVCGDLDSERYAGYHVQDCAAATT
                     NLLHAAHALGLGAVWGTVFPDKREPVRTLLGLPEHVIPFSIVPLGQPNEDAKPWKSRL
                     DTSRIHYNGWN"
     gene            1745..2593
                     /locus_tag="PLD82_07645"
     CDS             1745..2593
                     /locus_tag="PLD82_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOG65293.1"
                     /translation="MAEPLSPSRLYTCIDNRYGYAIHFLEGQKLIEDLALLRERTAPA
                     FAYLRQAVLSVLPMIVFLKRDEEFGFYVDSRDPNSILKIETRFDGRIRAMQSGEAPSN
                     EMALLSGICRIVKFLPGETTPYTSLVDFTDMPFDGIINITLARSFQFASRIILARESD
                     QSVLLSALPPLGSETPAKPAMMPDEYLKRVLPKLEGIIEPGLTREDTIIEAFSNIDCK
                     FLVARDVAFSCSCSKQRFVEVLRSLEASGQDPFENESVIETVCDYCGLRWEIARSDLA
                     TRSNIG"
BASE COUNT          604 a          844 c          718 g          617 t
ORIGIN      
        1 catgcggctt gttggccggc atgatcagca tctctcctgc ccggaccgtc tgcgcgtcac
       61 catcaatggt gatctccgcc tcgccgtcca gaatatgcac caccgcgtcg tagggcgagg
      121 tatgttcgga cagcccctgc ccggcgtcaa acgaaaagag ggtcaggttc ccggcttcct
      181 ttttgtcgag gatcgttttt gaaaccacgg agccctcggc gtaggccacg gattgtgccg
      241 ggtcgaaggc tgtcccaaca aagtttgttt ccataatgtg tgcccttgtt ttcagttttg
      301 tcccagcatc ccgcgcttga gaccggactg gacgggatcc ggcccaagcc agatggcaaa
      361 gagggccttt ttgaaggcaa gaccgggtat tgttccgagc gtcctctgtg actgcgttcc
      421 cttgacctgg taggatgccg tcagcccgct tcccggaaca taggtcaaat agatcagatc
      481 tccgacctgg atattgatcc ctgtaaaaaa tgcaagaaac cgctcccgct cgggggctgg
      541 atatcccagt gcggccacgt cctgaaaacc ctcacgggtc gccgacatga atcggtcacg
      601 tgtcagcttg gacgaatcaa ccttgaggat gacggacatc ggctcgtcgg cggccacaat
      661 cgcttcaccg gatctccctt tgagattggc gggaacatag aggctggcaa agtagatggt
      721 gacaaaaaat ttctcccgct cgccggttcc ggccaggacc atgtttttgc cgccaagctg
      781 ccagccatgg agcggaagaa tgaccgccac gaacatcaca atactgatat accgcaaaag
      841 tgtatttttg gggaacatac tcgcctccgt tcatgtgcgc gggtactcac gggtcgcgct
      901 cgagaaacgg tagcacatcc gcgtaccggc cgcaagggcc atggcacgaa gtgtcaacgc
      961 ccgctcaaat caggaactat tgctcaagta tcgccagaga ggctctgcca gaaaaggcac
     1021 aatccaccgc aggcggcggt tcttcaagca tcgatgcggc acgaggtgca aatcatcccg
     1081 gtctgtacaa taccggacaa ttcccggatt gtggacagtc cgtcagttcc acccgttata
     1141 atggatcctt gaggtatcca gccggctctt ccagggtttc gcatcctcat tgggctggcc
     1201 aaggggcacg atcgaaaagg gaatcacgtg ttcgggcagc ccgagaagcg tgcgcactgg
     1261 ctccctttta tcaggaaaaa ccgtacccca gacagcgccc agtccaagag catgcgccgc
     1321 atgcaaaaga ttggtggttg cggcagcaca atcctggacg tgatagccgg catagcgctc
     1381 gctgtccaga tcaccacaaa caagaattgc cacgggcgca tcggaaacaa acgcgacatt
     1441 gggattgagg gccgcgtact tttccagcag agtccggtca tcaaggacca caaactgcca
     1501 ggcctgctcg ttgccggccg aaggcgcatt catggccacg tccagcagac tggtcaccgt
     1561 ctcacggctg acagattttg gcagaaaacg ccggacactg cgccgactcc tgagttggtc
     1621 caggtttccg tcctgcatgg cattcctcct gatcgatccc agctacgagc ttcagctccc
     1681 gcgctgaatg ggaaaatcgt atctttatct tctgtttcat aaatatactg tcaaggaggt
     1741 tgtcatggca gaacctctct cccccagccg actctacacc tgtatcgata accgctacgg
     1801 atatgccatc catttccttg agggtcaaaa gctgatcgaa gacctggccc tcttgcgtga
     1861 gcggacggcc cccgcgtttg cctatctccg acaggctgtc ctttccgtcc tcccgatgat
     1921 cgtcttcctc aaacgggacg aagaattcgg cttttacgtc gacagccgcg acccgaacag
     1981 catcctcaag atagaaacac gttttgatgg acgtattcgc gccatgcaga gtggagaagc
     2041 cccttccaac gaaatggctc tcctctcggg catctgccgg attgtcaaat ttctacccgg
     2101 ggaaacaacc ccctatacct cgctggttga tttcacagac atgccctttg atggaatcat
     2161 caacataacc ctcgcccgct cgttccagtt tgccagccgg atcatcctgg cccgggagag
     2221 tgaccagagc gtccttctct ccgccctccc tccgctcggt tcggagacac ccgccaaacc
     2281 ggccatgatg ccggacgagt atctcaaacg cgttctcccc aaactcgaag gcatcatcga
     2341 gcctggcctg acgcgggaag acacgatcat cgaggccttc tccaatatcg actgcaaatt
     2401 ccttgtcgca cgtgatgtag ccttctcctg ttcctgttca aaacagcgct tcgtcgaagt
     2461 cctgcgttcc ctcgaagcca gcggacagga tccctttgaa aacgaatccg tcatcgagac
     2521 ggtctgcgac tactgtggac tgcgctggga gatcgcgcgc agtgatcttg ccacccggtc
     2581 caacatcggc tgaggtccct gtcaggctcg tcctgttctt tttggtgatg agcatgttgt
     2641 cacttggttt tgccttgcaa gaatccccgc ttccgggtac caaagacatg ccatcccgtc
     2701 acccggattg atatcagagg tgtatcggtg tagtctgctt tcatgaaacc accccggata
     2761 gtctctcccc ttcttgtcct ctt
//
LOCUS       DAOSHE010000277         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34778_length_2777_cov_3.710507, whole genome shotgun sequence.
ACCESSION   DAOSHE010000277 DAOSHE010000000
VERSION     DAOSHE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34778_length_2777_cov_3.710507"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2577)
                     /locus_tag="PLD82_07650"
     CDS             complement(<1..2577)
                     /locus_tag="PLD82_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65294.1"
                     /translation="MADSKQQNADSSEDSSFINALEQLAPPPPEGFHSLNAASGERDV
                     TDISEIPDITAFLDASSPDDDYLPAETAPLVETWVDGRRIDPRRTMFRLLGILASLVV
                     AVFLLLALVSLLGPSVPSSQGTALEQELAAGRQIIIETGPLGEIAPFLHPERLIPGVD
                     LDLLDEGQRVAPANYRLEFEWQVPQIIILLDLKNPATPLVRSRLKDLLQQITALITKK
                     RENEPDTLCSLWTWDQGRPVQRLAATPVQDDLLTGFADARGTWSAGESMLHTVLSTCT
                     NQRDSNQIVIHIADYPATISGKFSSEKLYTILKKHPRTAIAGLRLNPAAERDTRYEDP
                     VRYMAENARDFILPDWAFLSENVLTNTPPSLAFLLERKAPVRSIRFIYKPDQSILDAA
                     LEKPGMMRRISLSLRRYPRDLKLSALSSASFAPDVYKTHLTAQVHRSQSLQPETNSRF
                     RIIPREWIFGLTPGTRNIQYTAGRLAAASRSVLTAALPGGIFLAKSMESNATIPAAVD
                     GIGFTAPHLVFPELLEEASSVKSNLASRYYHTNRSAVLDVRTPLLLALTGLEGLSHED
                     FDDPASIPFGLNVSLSNNKTLQPATSILDALSQPSGLRFRNSSFLQLTPYPDQSEGKG
                     RIMVSGFEMLAVLDKLTRDPACSNGILFAGSAAVFPSSWKGLSAVQGQIADLLKKLAQ
                     RGIHVIIPWEGPTGEFSLPLPENLLAVHPVHTVDRIHSPIRAESFLQAPGSFALAPAL
                     LPDGKYAGPAVAAAWAAGMVARIRQSIPGIPVAGISDILLRSSEMRGDVLASRSFFNH
                     GILSPKILPEVISNRMEISGIPLRFSRMAQDPYHDAASLFCDGFLNSLAPGTLILPLP
                     GSL"
BASE COUNT          626 a          677 c          916 g          558 t
ORIGIN      
        1 caacgaaccc gggaggggaa ggatcagggt tcccggggca agactgttca aaaaaccgtc
       61 acaaaagagg gatgccgcgt catgataggg atcctgtgcc atccgggaga agcggagcgg
      121 aatgcccgag atctccatac ggtttgagat gacttccggg aggatctttg gagacaggat
      181 tccgtggttg aaaaaactgc gggaggcgag aacatcaccg cgcatctcgc tggagcgcag
      241 gaggatatct gaaattcctg caaccggtat ccccggtatg gattgacgga tcctggcgac
      301 catcccggct gcccaggccg cagccaccgc cggaccggca tatttcccgt cggggagaag
      361 ggccggtgcc agtgcaaagc tgccgggagc ctgaaggaag gactcggcgc ggatcgggga
      421 atgaatccgg tcaacagtat gaacgggatg gaccgcaaga agattctccg ggagcggaag
      481 gctgaactct ccggtcgggc cctcccaagg gatgatcaca tggattccgc gctgggcaag
      541 tttcttgagg agatcggcaa tctgtccctg aactgcactc agacccttcc atgaggaggg
      601 gaagactgcg gccgaaccgg caaataggat gccgttggag caggcgggat cacgggtcag
      661 tttgtccaac accgccaaca tctcgaaacc gctgaccatg atccggcctt tcccttcgct
      721 ctggtccgga tagggggtca gttgcaggaa agaagaattg cggaaccgga gtccggatgg
      781 ctggctgagc gcgtccagga tggaggttgc cggctggaga gtcttgttgt tcgagagaga
      841 aacattcaaa ccgaaaggaa tggatgctgg atcatcgaag tcttcgtggg agagtccctc
      901 cagtccggtc agagcgagaa ggagcggtgt ccggacgtca aggacggcag accggtttgt
      961 gtggtaatac cgtgaggcaa gattgctttt gacagacgag gcttcttcaa ggagttcggg
     1021 aaaaacaaga tgcggagcgg taaacccgat cccgtcaacc gcggccggga tggtggcatt
     1081 ggattccatg ctcttggcca aaaaaattcc gccgggaagt gcggcggtca ggaccgaacg
     1141 cgatgccgct gccagacgcc cggccgtgta ttggatattc cgtgttcccg gggtgaggcc
     1201 gaagatccat tcacggggga tgatccggaa ccgggaattg gtttcaggtt gaagactctg
     1261 gctcctgtgg acctgcgcgg tgagatgggt tttgtagacg tccggagcaa aggatgcgga
     1321 agagagcgca gaaagtttga gatcacgggg gtatcttcgc agggagaggc tgatccgacg
     1381 catcatcccg ggcttttcga gcgcggcatc cagtatggac tgatcgggtt tatagatgaa
     1441 gcggatcgag cggactggcg ccttgcgttc gagaagaaag gccaaggagg gcggtgtatt
     1501 cgtcaaaacg ttttcggaga ggaaggccca gtcgggaaga atgaaatctc tggcattctc
     1561 ggccatgtag cggacaggat cctcatagcg tgtatcccgt tcggctgcgg gattgaggcg
     1621 gagaccggca atggcggtcc gtgggtgctt tttcaggatg gtgtagagtt tttcggaact
     1681 gaatttgccg gaaattgtcg ccgggtaatc ggcaatatgg atgacaatct ggttcgaatc
     1741 acgctggttg gtacaggttg aaagaacggt gtgcagcatg gattccccgg ccgaccaggt
     1801 tccgcgtgca tcggcaaacc cggtcaggag gtcatcctgg accggcgtcg ctgcgagtct
     1861 ctggacaggt cggccttggt cccaggtcca gagggaacac agggtgtcgg gctcattctc
     1921 ccgttttttc gtgatcaagg ctgtgatctg ttgcaagaga tccttgagcc tggaacggac
     1981 caagggcgta gcgggatttt tcagatcgag gaggatgatg atctgcggga cttgccattc
     2041 gaattccagt cgatagttcg cgggggcaac acgttggccc tcgtccagaa gatcaagatc
     2101 gacaccggga atcaggcgct ccgggtggag aaatggcgca atctccccga ggggaccggt
     2161 ttcgatgatg atctgacgac ctgcggccag ttcctgttcg agggcggtcc cctgtgacga
     2221 ggggacagat ggaccgagaa gactgacgag ggccagaagc agaaagacgg ccacgaccag
     2281 gctggcaagg ataccgagaa gccggaacat tgtccgcctt ggatcgatcc gtcgtccatc
     2341 gacccaggtt tcgacaaggg gcgcggtctc ggccgggagg tagtcgtcat ccggtgagga
     2401 tgcgtcaaga aaggccgtga tgtcggggat ctcgctgata tccgtcacgt cccgctcgcc
     2461 cgaagcagca ttcagtgaat gaaagccttc cggcggggga ggcgcgagtt gttccagcgc
     2521 gtttataaag cttgaatctt cgctggagtc cgcattctgt tgtttgctgt ctgccatgcc
     2581 accctcacaa cggacattct atcgatgcat ccggtccctg tcaatggaag atgcagtttc
     2641 tgaatgaagt tctgttcaac agtgcacggg aaatggggtg tcacaatcac ccttgggctc
     2701 ttgtcaacgg gttcggaact ggtcgatgaa acccgattga cagccggaag aagaaagaat
     2761 acaatgttga tatcatg
//
LOCUS       DAOSHE010000278         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_34797_length_2776_cov_4.103271, whole genome shotgun sequence.
ACCESSION   DAOSHE010000278 DAOSHE010000000
VERSION     DAOSHE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34797_length_2776_cov_4.103271"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..553
                     /locus_tag="PLD82_07655"
     CDS             188..553
                     /locus_tag="PLD82_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65295.1"
                     /translation="MAEKVLIVDDSAIMRNLVRQTLSSGGYEVVLANDGQQGLDAWRS
                     SAGSVDLVITDINMPVMDGITLIGEIRKIDEDVPILALTSESDVEMRNKGVDAGANGW
                     IVKPFHGAQFLDIVRQILS"
     gene            complement(1189..1659)
                     /locus_tag="PLD82_07660"
                     /pseudo
     CDS             complement(1189..1659)
                     /locus_tag="PLD82_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(1777..2049)
                     /locus_tag="PLD82_07665"
                     /pseudo
     CDS             complement(1777..2049)
                     /locus_tag="PLD82_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(2086..>2776)
                     /locus_tag="PLD82_07670"
     CDS             complement(2086..>2776)
                     /locus_tag="PLD82_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65296.1"
                     /translation="PTFQAISDRLGEMNVAMQNVQRTSAETAKVLKTIDEISFQTNLL
                     ALNAAVEAARAGEAGAGFAVVASEVRALAQRAATATTDTNRLVEDSVNAARTSSEAFA
                     EVSKALSANQTLSQKVVQLVADITTMNREQAQGIEQVNKAVSEMEIIVQQNAANAEET
                     AASTEELSGQAGDLKSMAGDLAGLIGQTEHLELAAPVRGPVRQGVQHRPAPDPGTETV
                     RQLDQDDLDSF"
BASE COUNT          510 a          720 c          840 g          706 t
ORIGIN      
        1 cgagtcactt tctggctacc tcgagaattt tgtgatccgg gagttcgggc tggcgagata
       61 ctatgaggtc aatgcgtcag gtgtatacat attcaaacgg aagagcaccg tttgaccagg
      121 atttgacagg cgtattggaa atttggcggt ttcggatggg cggccgcatg gcaaggtgga
      181 gggaacaatg gcagagaagg tactgattgt cgatgattct gcgatcatgc ggaatctggt
      241 gcggcagaca ctctcttcag gcggatatga ggttgtcttg gcgaatgacg gacagcaggg
      301 attggatgca tggcgttctt cggccggatc cgtggatctg gttattaccg acatcaatat
      361 gcccgtgatg gacgggataa cattgatcgg cgagatccgg aagatcgatg aggacgtgcc
      421 gattctggcg ttgacatcgg aatcggacgt agaaatgcgg aacaaaggtg tagatgccgg
      481 agcgaatggg tggattgtga agccgttcca tggtgcccag tttttggata ttgtcagaca
      541 gattctttcc tgaagcagca ggagtgcgta aaaaatgggc atttttccct attccgtttt
      601 tgacaaaagg gtgcgatact gacagggata tatgactcct tgtgggtttt gcatcaagac
      661 gcgtccgttg gaccgtctcc gtccttcagt gaccgtcctg tcggtttttc cggccgggcg
      721 gtcatttttt cggtctgcgc tgtggggcgc ccggctggct ccggagcgat atcttgccgc
      781 cgctatggtg cagtttttcg tccatacact catgaaatgc ctgttttctc gtagacggcg
      841 actttccggg ctggttttgt tctggtgaac tccctcatgc ctgtgggagg acgagtttct
      901 ggtcagaatg agccttcgat gaggggtttt cgcctccgcc atggtgcatt ttcctgttct
      961 tgcgctcttt tggccttcgc catggtgcat ttttcgttca gctaaggcta ctgacatgca
     1021 actatggtga cattttcgag tgctgccgtt tttcggaccc tcgctatggt gcttttctct
     1081 tcacagcaac gaatccgacg gcggactatg gtgcattgtt gagtgcagtt gcccgccaca
     1141 gggcggcatg attggttcca ctggccgcat ccagagtacc ccgcctcttc agcatgtatc
     1201 agtccggagt tcccggattt tggcgaggac ggccgtggcg tgtccggcga ctttgacgtt
     1261 gcgccagaca tggacgatta cgccttccgg attgaccaaa aaagtcgaac ggacggttcc
     1321 catatactcc ttcccgtaat tgaccttttt ttgccagacc ccccagcgtt cctggacgag
     1381 gtgttccctg tccgaaagaa gtcgcacggt gatttccttt gcagaacgga accggcagtg
     1441 gctggccggg gaatcgctgc tgattcccag aattgtggca ccatggcgtg caaaatcatc
     1501 gaccagtctg ctgaactcct gcccctcaag agtgcacccg ggcgtattgt ctttggggta
     1561 gaaatagaca acgagccagc gccccttcag gtccaggagg tgcaccatgg tgccgtcttg
     1621 atccggaatc tccagagcag gggccggttg tcccggttca agacggggtg tcccgccctt
     1681 ggcctgtttt tttgcatccg gctcggcgga attttttggc gagggttttc gtgaagacgg
     1741 cttttttgtt gtactgagtt catcccggct cgcttgcagg agacgccgga gaaaatcctg
     1801 gtcatccaac tggtcttcga tcacaaaggc gtatttagct cccggatagg gtggggcttc
     1861 agtcggtctg tcaaggagaa cacgaccggc ttcggttgat ttgacaaaaa atctgttgtc
     1921 gcagatcagc ccgacaagca cgccatccag gtagagggcg tattcgccaa acatctttcg
     1981 cgtcatgatc tcgccggcgg atttgcaggc atcctgtatc agcgcaacaa aatcgggtgt
     2041 ggtcgccatg cattctcctt tgaatatctg ccgggactga ctcgatcaga agctgtcgag
     2101 atcgtcctgg tccagttggc gaacggtttc cgtccccggg tcgggcgcag gccggtgctg
     2161 cactccctgc cggaccgggc cccggacagg ggcggccagc tcgaggtgtt cggtctgtcc
     2221 gatcagtccg gccagatcac cggccatcga cttgaggtca ccggcttggc cggagagctc
     2281 ctcggtcgag gctgctgtct cctcggcatt ggcggcattc tgctggacaa tgatctccat
     2341 ctcggagact gctttgttga cctgctcgat cccctgggcc tgctcgcggt tcatggtcgt
     2401 gatgtcggcc acaagctgca caaccttttg tgagagagtt tggttggccg aaagggcctt
     2461 gctgacttcg gcaaaggctt cggaggatgt ccgtgccgcg ttgaccgaat cctcgaccag
     2521 ccggtttgta tccgttgtag cggtggcggc ccgctgggcc agggcacgga cttcggaggc
     2581 caccacggca aagccggcac cggcctcgcc ggctctcgct gcctcgactg cggcattgag
     2641 ggcgaggaga ttggtctgga atgatatttc gtcaatcgtt ttcaggacct ttgcggtttc
     2701 ggcactggtg cgctgaacat tttgcatcgc gacattcatt tcgccgaggc ggtcgctgat
     2761 ggcctgaaag gtcggc
//
LOCUS       DAOSHE010000279         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35106_length_2760_cov_3.077264, whole genome shotgun sequence.
ACCESSION   DAOSHE010000279 DAOSHE010000000
VERSION     DAOSHE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35106_length_2760_cov_3.077264"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLD82_07675"
     CDS             <1..504
                     /locus_tag="PLD82_07675"
                     /inference="COORDINATES: protein motif:HMM:NF023958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOG65297.1"
                     /translation="ERVSQGLCRRLAAHGPVYVNVHFNHTDELTEEARLACRRMNAAG
                     LITGNQTVLLAGVNDSVSQLRELFFGLLSCGVRPYYLHTMDHVGGTAHFRVPLSRAAA
                     LWEDLALTTSGMAVPRMMIDLPGGGGKIQYMRGMLAEDHGTGRLVFRNVDGGVTPYRD
                     LPDGGPQ"
     gene            501..1715
                     /locus_tag="PLD82_07680"
     CDS             501..1715
                     /locus_tag="PLD82_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65298.1"
                     /translation="MKRGAQSGAIVGVVAFHSRVRRGIAEQGGLTLALASAIMEQLER
                     VHAWCAQHGRRIDSLPPMTRRAVEDLTRIIMEAGSGKGEIREEASPVERGWSVPGLQR
                     KVTGWLDRLAAARNPAARTLYREDALEWMASLRSLMEYKGCRQDDLPVNEQRLLARFS
                     AWCETPWHEVIPAAAEVFEGTLMEGMPPAFFGMKTAQRPGLRYLPVDGRLWQVTRRGR
                     LPEFSISEMFLLDGSLEVPRRLARLLSGQAGRLAARERRFLRDWIRSGTVVDYILRIG
                     RDVCPGEGGARGMVRDLVGYWERINERDFAGRLGRPDLGWTRRVAHCRTGYYDAMTDR
                     IVISAALDDAAVPEYVLDFVLYHEMLHQASRLDILTGRGRVHDRDFRIEEQRHPRYHE
                     AEAFLRGLAHKR"
     gene            1800..>2760
                     /locus_tag="PLD82_07685"
     CDS             1800..>2760
                     /locus_tag="PLD82_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65299.1"
                     /translation="MKRTFRGMSLVMMVLVTACGGGVRPEGSQPEVPQAGILVQHAHR
                     TAVLLTAMDQAGHGMASMDETGEVCLWDGDGRIQARIPGQGGEGSALAFSPDGSLLAI
                     AWRDVLSFTQRVAVYRIGGRQDNASVWIRTHPVSMLQAQVPQAVTAMDWNPAGTTLAL
                     VARDGVRTWNVGTQELVALKIPGWNERNCVALAFAGPGSFLVADEAGTVTVIPATGAI
                     RERVVSSGLGPVTALAGGTDGEILIGHASGRIRVIGAGDGQMRDLFLGGTIAGFVRQG
                     LARDALVFDGRRALLLTIAGAKRMERILPGQVRGERAVTIRADG"
BASE COUNT          472 a          773 c         1002 g          513 t
ORIGIN      
        1 gagcgggtca gtcagggtct ctgcaggcgg ctggccgccc acggacccgt ctatgtgaat
       61 gtccacttca atcacacgga cgaattgacg gaggaggccc ggctggcttg ccggcggatg
      121 aacgcggccg gcctgatcac gggaaaccag acggtcctcc tggccggggt gaatgactcg
      181 gtcagccagt tgcgggaact ctttttcggt ctcctctcct gcggggttcg tccctactac
      241 ctgcacacca tggatcacgt cgggggcacg gcgcactttc gggtgccctt gtcccgggcc
      301 gccgcacttt gggaggatct ggcactcacg accagcggga tggccgtccc gcgcatgatg
      361 atcgatctcc ccggaggggg cggaaagatc cagtacatgc gcgggatgct ggccgaggat
      421 cacggaacag ggagactcgt gttccggaat gtggatggtg gcgtgacacc ataccgggat
      481 ctgcctgacg gaggtccaca gtgaagcggg gggctcagtc gggggcaatc gtcggcgtgg
      541 tggcgttcca ttcgcgggtg cggcgcggga ttgcggaaca gggcggtttg accctcgccc
      601 tggcgtcggc catcatggaa cagctcgagc gggtccatgc ctggtgcgcg cagcacgggc
      661 gacggatcga cagcctgcct ccgatgaccc ggcgtgcggt tgaggatctg acacggatca
      721 tcatggaagc ggggagcggg aagggtgaga tccgggagga agcatcaccg gttgagcgcg
      781 gctggtcggt cccgggattg cagcgaaagg tgaccggttg gcttgaccgt ctggccgctg
      841 cccggaatcc cgctgccagg acactctatc gggaggatgc gctggaatgg atggcttcct
      901 tgcgttcctt gatggagtac aagggctgcc ggcaggatga tctgcctgtc aacgaacagc
      961 gtctgttggc gcgtttttcc gcctggtgcg aaaccccatg gcacgaggtg attccggcgg
     1021 ctgccgaggt gtttgaagga accctgatgg agggcatgcc gccggcgttt ttcggcatga
     1081 agacggccca gcggcccggc ttgcgctatc ttccggtcga tggccggctc tggcaggtga
     1141 cgcgccgtgg gcggcttccc gagttttcga tatcggaaat gtttttgctg gacgggagcc
     1201 ttgaggtgcc acggcgcctg gctcgtctcc tctcggggca ggccggccgg ttggccgccc
     1261 gggaacggcg ttttttgcgg gactggatcc ggagcgggac ggttgtggac tacatcctcc
     1321 gtatcggcag ggatgtctgt cccggcgagg gaggggcgcg ggggatggtg cgtgacctgg
     1381 tcgggtactg ggagcggatc aacgagcggg attttgcggg taggctgggc cggcccgatc
     1441 tgggctggac gagacgggtg gcgcattgcc ggaccgggta ctacgatgcg atgaccgaca
     1501 ggatcgtcat ctcggccgct ctcgacgatg ccgcggttcc ggaatacgtg cttgattttg
     1561 tgctgtacca cgagatgctg caccaggcat cccgcctgga tattctgaca ggccggggcc
     1621 gggtgcatga cagggatttc aggattgagg aacagcgtca tccccgctac cacgaggctg
     1681 aagcgttttt acgcgggctg gcccataaac gctgatcggc ccggtgacta tatttccgga
     1741 gtgcccgcaa caacgggaat ccagactgtg cagaatacaa cgatgatcag aggtgtttga
     1801 tgaaacggac cttccgggga atgagtcttg tcatgatggt gctggtgacg gcctgtggag
     1861 gcggggtccg gcccgagggg agtcaaccgg aggtgccgca ggccggaatc ctggtccagc
     1921 acgcacaccg gacagccgtt ctcctgaccg ccatggacca ggccggacac ggaatggcca
     1981 gcatggatga aacgggcgag gtctgtctct gggacggcga cggacggatc caggccagga
     2041 ttcccggtca aggcggcgag ggaagcgcgc tcgcgttttc tccggacgga agcctgctgg
     2101 ccattgcctg gagggacgtc ctttccttca cgcagagagt cgccgtctac cggatcggcg
     2161 ggagacagga caacgcttct gtctggatcc ggacgcatcc ggtgagcatg ctgcaggccc
     2221 aggttcccca ggccgtgacc gccatggact ggaatccggc gggaacgacc ctggcactcg
     2281 tggccagaga cggtgtccgg acatggaatg tcggtacaca ggaacttgtt gccctgaaga
     2341 tccccggctg gaacgagagg aattgtgtgg cgctggcatt tgccgggccc ggttcgtttc
     2401 ttgtggccga tgaagccggg acggtcaccg tgattcccgc gactggagcg atacgggaac
     2461 gggtcgtgag cagcgggttg ggaccggtga cagcgcttgc cgggggaacg gacggggaga
     2521 tcctgatcgg tcatgcctcc gggcggatcc gggtcatcgg tgcaggggac ggacagatgc
     2581 gagacctctt tttgggagga acgattgccg gatttgtccg acagggcctc gcccgggatg
     2641 cccttgtctt cgacggaagg cgggccttac tcctgaccat cgccggagca aaacggatgg
     2701 agcgcatcct cccggggcag gtccgcggag aacgcgctgt caccatccgg gccgacggac
//
LOCUS       DAOSHE010000280         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35341_length_2746_cov_4.899666, whole genome shotgun sequence.
ACCESSION   DAOSHE010000280 DAOSHE010000000
VERSION     DAOSHE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35341_length_2746_cov_4.899666"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLD82_07690"
     CDS             <1..448
                     /locus_tag="PLD82_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65300.1"
                     /translation="PYGDSFNIYPIMGEILKMYSHAALPSGGIERIISGGVSRWTSNG
                     INLTTILNSLLTYADHKAREYVSFEQERAFSLQYQVAEKTATTITIKGQAWPGVEVAS
                     GVTIRGVVRTIKYRIADKALLSDILDINARNSNGTGWYFSANLRML"
     gene            929..1825
                     /locus_tag="PLD82_07695"
     CDS             929..1825
                     /locus_tag="PLD82_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65301.1"
                     /translation="MSGSLAINTGDRRSSGAWLPFFGPVLPGFRRPIAGLRLFTKKAV
                     LILLLLSSLTHLHAAPGATHDWLGELSLAGTLNSAGLALELELVRRFDISDPSAWLTS
                     EANIGLGTFISLYPVGYHTALRLVVSPLLFLDAGVHAGVHGSWEMYRYTSLPVRYGED
                     ERDGLPFSTENMAWLSPFLVLKAKFGPWILYNSFWFERYFGPDGWWLNWYHEAVMRDG
                     WLINVNNYLMYEFSPRWRLAAFSRYWYVRDSRETKWLVGMAPSFLLGGGIRCTIGFGW
                     HVREVNYDGIKVMGALSLPLEW"
     gene            2005..>2746
                     /locus_tag="PLD82_07700"
     CDS             2005..>2746
                     /locus_tag="PLD82_07700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG65302.1"
                     /translation="MNQTLLGEKIAGRYRLSSLIGEGGMSRVFEAEDLHDESRRVAVK
                     ILKTGTVSARLEDVIRFRNEAEIVSRLDHPGIVHIYQVGQHRGMHYLVTEYLEGACLA
                     ALLGSGKVEIRTTITLMACLAEALRHIHDKGIIHRDIKPANIIVHEEGRDGYDASLTL
                     IDFGLARIREFSRMEDSDEIVGTFAYMSPEQSGVLKRKVDERSDLYSLGIIMYQMLTG
                     VLPFSGDSISTFEVEILDEKQLRKIGMRA"
BASE COUNT          543 a          752 c          787 g          664 t
ORIGIN      
        1 gccgtatggt gacagcttca acatctaccc gatcatggga gagattctca aaatgtattc
       61 ccatgcggca cttccgtcag gcggcattga gcgcatcatc agtggcgggg tttcccgttg
      121 gacgtccaat ggcatcaact tgacaactat cctcaactct ctcctgacgt atgcggatca
      181 caaggcccgc gaatatgtga gctttgaaca ggaacgggca ttcagtctgc agtatcaggt
      241 cgccgaaaaa accgccacga cgattaccat caaagggcag gcttggcccg gtgtggaagt
      301 cgcttcgggt gttaccattc gcggcgtggt tcgcaccatc aagtatcgga ttgcggacaa
      361 agcgcttctt tcggacatcc tcgacatcaa tgccagaaac agcaatggca cgggctggta
      421 tttcagtgcc aatctgcgca tgctctgagt cgttccttgc agcctgtcgc gagcaaccgc
      481 tgcttccaag ggaggcgcgg ttgttttttt ctcgattccg tgaattcatc gttgatacca
      541 tggtgccagc gaagtcaatt gcatgcaatt caaacaactg cgtactgatc ctgcggggac
      601 ttcgccggga ttatgggcgt attgcgtgaa ggtggcagcg ggggtacact gcgtaaaatt
      661 tacgcaattt gacaatccgg gatcgcgtca aaaaacacag agcagaaaac acatgcaaaa
      721 aaccacagtg aatttgaggt ctttgtgaat aacagcagaa attggttcga acccgttgcg
      781 taaaaaattt aagcaaaata cgtactccgg gattgcacga ggtctgtgct tgtttttgat
      841 ttcccttcac acaccgtcgc cggtgcatgc cgggcttgca ctttatggtg catccggtat
      901 actcggaggt acgacagatg ggaggcctat gtccggatcg ctcgcaatca acactgggga
      961 ccgtcgcagc tccggcgcgt ggctgccgtt ttttggaccg gttcttccgg gttttcgccg
     1021 gcccattgcc ggactgaggc tgttcaccaa aaaagccgtg ctcatcctcc tgctcctctc
     1081 ttctctgacg catctgcacg cggcccccgg ggcgacgcat gactggctgg gagagctgag
     1141 cctggccgga accctcaaca gtgccgggtt ggcgcttgaa ctcgaactcg tgcggcgatt
     1201 tgacatttcc gatccctcgg cctggctcac cagcgaggcc aatatcggtc tcgggacctt
     1261 catctcgctc tatccggtcg gctatcacac ggcgttgcgc cttgttgtca gccccctgct
     1321 gtttctggat gcgggcgttc acgccggagt ccacgggagt tgggagatgt atcgctacac
     1381 cagcctgccc gtccgctatg gcgaggatga acgtgatgga ttgccctttt ccactgaaaa
     1441 catggcctgg ctctcgccat ttctggttct caaggcgaag ttcggaccgt ggattctcta
     1501 caactcgttc tggtttgagc gctatttcgg tccggatggc tggtggttga actggtatca
     1561 cgaggcggtc atgcgcgatg gctggctgat caatgtcaac aattacctga tgtacgagtt
     1621 ttctccccgc tggcgtttgg ccgccttttc ccgctactgg tacgtccggg attcccggga
     1681 gacaaagtgg ctggtcggaa tggctccttc tttccttttg ggcggcggta tccgctgtac
     1741 gatcgggttt ggctggcatg tccgcgaggt caattatgac ggaatcaagg tcatgggcgc
     1801 cctctccctg ccgcttgagt ggtagcctgt ccgtttctct cattttcgtg gccttctggc
     1861 cggaaacccg atctgcctgc agtgttcatc ggctggcgat accgggatgg gccggatgcc
     1921 gcatccgtcc aggctggtga aattcatttt tccggttatt tgttcctgtg gtacactgtt
     1981 ctccgaccat accgggagtt gcgtgtgaat cagactttgc tgggagaaaa gattgccggg
     2041 cgttatcgct tgtcgtccct gatcggagag ggcgggatga gccgtgtctt cgaggccgaa
     2101 gacctgcatg atgagtcccg ccgggtggcc gtcaaaatcc tcaagacggg gacggtttca
     2161 gccagacttg aggatgtcat ccgcttccgg aatgaagccg aaattgtctc ccgccttgac
     2221 catcccggta tcgtccatat ctaccaggtc ggccagcaca ggggcatgca ctacctggtc
     2281 accgagtatc ttgagggggc ctgtcttgcg gcccttctgg gttccgggaa ggttgaaatc
     2341 cgcacaacca tcaccctgat ggcctgtctc gccgaggctt tacggcatat tcacgacaag
     2401 ggaatcatcc accgcgatat caagccggcc aatatcattg tccacgagga gggcagggac
     2461 gggtacgacg ccagtctgac cttgattgat ttcggtctgg cccggattcg cgagttttcg
     2521 cggatggagg acagcgacga gatcgtgggc acgtttgcct atatgtcgcc cgagcagagc
     2581 ggggttctca aacggaaggt cgacgagaga agcgatctct attccctcgg gatcatcatg
     2641 taccagatgc tgaccggggt tcttccgttt tccggcgaca gcatcagcac cttcgaggtc
     2701 gagatcctcg acgagaaaca actccggaag atcggcatgc gggcgc
//
LOCUS       DAOSHE010000281         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35370_length_2745_cov_3.643123, whole genome shotgun sequence.
ACCESSION   DAOSHE010000281 DAOSHE010000000
VERSION     DAOSHE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35370_length_2745_cov_3.643123"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..774
                     /locus_tag="PLD82_07705"
     CDS             265..774
                     /locus_tag="PLD82_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG65303.1"
                     /translation="MSELDEALAVFSSRIDTSCPPGCITCCQYPDVQATVLEFLPLAC
                     RLLEEGRAESILDRIEHQPETLPGCVFADETGCPVYQDRGLICRLFSASVRVDRLGRL
                     EYVACSILHTRHGRHLPAETLPPVMSDWGLRLEAIDPVLGSHWYPVNEAIRRALEMVL
                     YSNHLDQAG"
     gene            complement(891..1316)
                     /locus_tag="PLD82_07710"
     CDS             complement(891..1316)
                     /locus_tag="PLD82_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65304.1"
                     /translation="MKSGRVQTWVMLVVLGLISCGPDQAIVKGDTQQGAADSAQSSSA
                     GTKPASAEVTSLAGRWKLNNKNERAIWFFDTTGRCAIRPSGLVEQKGRYQYDGKHLVI
                     NFDSGTTRYTVLEKTEPNLVIMMRWGDLYNITNVLTRIP"
     gene            complement(1497..>2745)
                     /locus_tag="PLD82_07715"
     CDS             complement(1497..>2745)
                     /locus_tag="PLD82_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOG65305.1"
                     /translation="EELDIIDAGYGVYREAFKTGKWSVILHACYHGDNSQMFYSEGVI
                     VTSDTSLHTFEEAAKRPVYAVSPSSATGWQLQQAMFERAGVHPGKITFVKKHTDVPAM
                     VAAHPGSIGFCGAFADYARHGLRVLARTPRVPSALVIARQSVPGSLQTEIAAVLKDFF
                     LEEYRADPVFIRRFYVFPLEQDYSTYFSAFAKPEPVSPVPILFLVLLTVLTGVVVFLT
                     MLLRRTNKKRIIQACGYEEARLTLERSGFGSSWKKNCLHIFDKSVLALGEEAWDTVLV
                     TITRDIERILPALVHRVNALPVSDTWKQVLSERMDTWALILSFNKRVVEHVRALLAKS
                     RVQTDLALAYTDILTPEEYPLSHFACLDGLLISLYARRNLVVHAHARFISREEARYTL
                     DSWLKLQATLAESGLFSESGESG"
BASE COUNT          587 a          769 c          826 g          563 t
ORIGIN      
        1 ggatattggt catcagggca ttttcccgga tgaccaggag gggaaacgag agctcctcgc
       61 ggagaagatt caacgagagg gcgggtatcc gcgccacggg aaggccatcc gcgatcggcg
      121 gaagtccctt ggccggagtt gacaggacgg attgtcggat tgaagaaaga tccagcatat
      181 ttgtttgctc cgaaatgaaa ttcgtgagta gacttcatca ccatgagacg gatagcgtat
      241 ttatcgggcg aagtgcgttg cttgttgagt gaactcgacg aagcattggc cgtgttttcc
      301 agccggatcg acacgagttg tccgcccggc tgcatcacgt gttgccagta tcccgatgtc
      361 caggcaacgg ttctcgaatt cctgccactc gcctgccgcc tgcttgaaga aggccgggcc
      421 gaatcgatcc ttgaccggat cgaacaccag ccggagactc tcccgggatg cgtctttgcg
      481 gatgagaccg ggtgtccggt ctatcaggat cgaggcttga tctgccgtct gttttcggcg
      541 agtgtccggg tcgatcggct cggtcggctg gaatacgttg cctgttccat cctccacacc
      601 cgccatggac gacatctgcc tgcagagact ctcccgcccg tgatgtcgga ctggggcttg
      661 cggctggaag cgattgatcc cgtcctcggt tcgcactggt accctgtcaa tgaagcgatt
      721 cgccgggctc tggagatggt cctctactca aaccacctgg atcaggccgg ataaggcgga
      781 gcaagggcgt cagcatccgg ttctgtttga tgaacaccct cccgccacac gggatccggc
      841 tggcagggag gatgtgcggt cgaacggggt ggcagccccc cccggggtga tcacgggatc
      901 ctggtcaaca cattggtaat attgtagaga tcaccccagc gcatcatgat gaccagattg
      961 ggttccgttt tttcgaggac cgtgtagcgt gtcgttcccg aatcaaaatt gattacaagg
     1021 tgctttccgt catactggta gcgacccttt tgctcgacca acccgctcgg ccggatggca
     1081 cagcgtccgg tggtatcaaa gaaccagata gcccgctcgt ttttattgtt gagcttccag
     1141 cggccggcga gggatgtcac ctcggccgag gccggttttg tccccgctga gctggactgg
     1201 gcgctgtcgg cagccccttg ctgtgtgtca cccttgacga tggcttgatc cggtccgcat
     1261 gaaatcaatc cgaggaccac aagcatcacc cacgtctgca ctcttccact cttcatgtcg
     1321 cctcctgaaa cgtgcctcca gtatactccg gaacatggtc cctgtaaagg agagaatgcc
     1381 gtcggggcag aacaggcccg gacaggtccc tgcccgggga aaatcgcgca cccggcccac
     1441 gtgaaaacct ttgtctgtcg cggagacagg aacaacaggc cgggcgttcg ccagattcag
     1501 ccgctctccc cggattccga gaagagtccg ctttcggcca gggtggcctg cagcttgagc
     1561 caggaatcca gggtgtagcg tgcctcctcc cgactgatga atctggcgtg cgcatgaacg
     1621 accagattgc ggcgggcgta gagcgagatc aagaggccat cgagacaggc aaagtgcgag
     1681 agcgggtatt cctccggagt caggatatcc gtataggcca gagccaaatc ggtctggacc
     1741 cgactctttg cgaggagtgc ccggacatgc tcgacaacac gtttgttgaa cgagaggatc
     1801 agcgcccagg tgtccattcg ttccgacaga acctgcttcc aggtatcgct gaccggaaga
     1861 gcattgaccc ggtggacaag cgccgggagg atccgctcga tgtcgcgggt gatggtgaca
     1921 agcactgtgt cccaggcctc ttccccgaga gcaaggacgg atttgtcaaa aatatgcagg
     1981 caattttttt tccaggatga accaaagccc gatcgttcca gcgtgagccg tgcctcttcg
     2041 tatccacacg cctggatgat ccgcttcttg tttgtccggc gcagaagcat ggtcaagaag
     2101 acgacgactc ctgtcaggac ggtgagcagc acgagaaaga ggatcggcac aggagaaaca
     2161 ggctccggct tggcaaaagc gctgaaatag gtgctgtaat cctgctccag cgggaagaca
     2221 taaaagcggc ggatgaagac aggatcggcc cggtattctt cgagaaaaaa atccttgagg
     2281 actgcggcaa tctcggtctg gagagaaccg ggaacggatt ggcgggcgat gaccagggcg
     2341 ctcggcaccc ggggtgtccg tgccaggaca cgcaacccgt gccgggcgta gtccgcaaac
     2401 gcgccacaaa agccgatgct tccgggatgg gctgcaacca tggccgggac atctgtgtgt
     2461 tttttgacaa aagtgatctt ccccggatgg acaccggccc gctcgaacat ggcctgttgc
     2521 aattgccagc ccgtggccga agagggactg acggcataga ccggtcgttt ggcagcctct
     2581 tcaaaagtgt gcagagatgt gtcggaggtg acaatgaccc cttcactgta aaacatctgg
     2641 gaattgtcgc catgataaca ggcatgcaaa atcacggacc acttcccggt cttgaatgcc
     2701 tcgcggtaga cgccataccc cgcatcgatg atgtccagtt cttca
//
LOCUS       DAOSHE010000282         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35591_length_2733_cov_3.403286, whole genome shotgun sequence.
ACCESSION   DAOSHE010000282 DAOSHE010000000
VERSION     DAOSHE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35591_length_2733_cov_3.403286"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLD82_07720"
     CDS             complement(<1..475)
                     /locus_tag="PLD82_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65306.1"
                     /translation="MDNAGKDLHLWAMRMNWLRWLARPLVSVWYGLVFAFQYFWHLVR
                     VVTRAVRDSFSLRYWTFCLFGLAIAIFFAFSFWFLSVKLGQPWLAWLGVLFAILPVGW
                     AMAEISSRERRRLAMDDEYHEDSLYLGNGLKAVRILLKYVILSLFLVVLLILGTLL"
     gene            complement(658..>2733)
                     /locus_tag="PLD82_07725"
     CDS             complement(658..>2733)
                     /locus_tag="PLD82_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOG65307.1"
                     /translation="DDHTVASILVRLRSFKQSVSETALEYVKKEVTAAGFADADVFIA
                     GEPVVTTLLGKFMVRDLEILFPFVLIVVLTSLVVSFRRLGATILPLIGVLYATVITIG
                     LMAMTGSSITVISVVLPVLMIAVGSAYAIHVVNAYLLHGGSDMHKAVRESVTETFVPV
                     LMAAFTTVGGFASLASNSIIPIRDFGIFTAIGVLAAFILAVLFVPALAILMRMKPMQE
                     THEESADGKILGMLSGVSKRHVWPVFTVGVLFVVISGIFAGSIRVDTNAVRMFQSDTE
                     IRKADAFINTHFKGTSTAEILIHTDTENGAFEPKLLRIVDGLEDWLMTNAAVPAEVRS
                     NFGGALHLADYIKKANRSLHGEKKDWYRIPDSRRAVMDYMLILRGATDDLVSRDGKTL
                     RVVVTMKDGSTVLAGELKASIGKYMAANNLPDFEFLGVMAKYLQVDRLIVLGQIVSIL
                     LSIIAVFLLNAVVFRSIFWAAVSLVPLSMAIAVNFGIMGAFGLLLDVGTSMIASIAIG
                     IGVDYSIHFINGMLVRVRRGMSLDEAMHRTGMQTGRAILFNMVAVMAGFFVLAFSSFV
                     PLINFGILIGITMITTGLASLLVLPAFIRILAKTACFTKVSGISAGSSRGTSSGLKAA
                     KTPAVDSSRPERKTARSAGAGSSTKKSASRMTRMATRGNEVVPQKKAKTAPAPKKKAV
                     SRGKPKTDG"
BASE COUNT          683 a          761 c          768 g          521 t
ORIGIN      
        1 ggaggagcgt cccaaggatc aggagcacga ccagaaagag cgagagaatg acgtacttga
       61 gaaggatccg gaccgccttg agcccgtttc cgagatagag cgagtcttca tggtattcat
      121 catccatggc cagtctgcgt cgttcacggg acgagatctc ggccatggcc cagccaaccg
      181 gaagaatcgc aaaaaggaca ccgagccagg ccagccaggg ctgaccgagt ttgacggaga
      241 ggaaccagaa ggaaaaggcg aaaaagatgg caattgccag tccaaagagg cagaaggtcc
      301 agtagcggag ggaaaaggaa tcacgaactg cacgggtcac aacgcggacc agatgccaaa
      361 aatattggaa ggcaaagacg agaccatacc agacggaaac aagaggtctg gccagccaac
      421 gcagccagtt cattctcatt gcccagaggt gcaggtcttt cccggcattg tccatcgtcg
      481 tttcctcgcc ccttctttgt atcgcaccct gtgccaactg tcaatccgta ccgcaaggca
      541 cagaaaagga cctcttcagt atcggcaggg cgccccttga atcttgaatc cggaccacag
      601 gcaaaaaaaa cacccgcccg gagtcgggac aggtgtttga aggtcaatac gggatgctta
      661 cccgtcggtt ttcggcttcc cccgggagac tgctttcttt ttgggggctg gcgctgtctt
      721 tgcctttttt tggggaacaa cctcattccc gcgggtagcc attcgggtca tacgggaagc
      781 ggactttttt gtggaagaac ctgctcccgc gctgcgtgcg gtctttcgct ccggtcggga
      841 ggaatccaca gcgggcgtct tcgccgcttt caggcctgag gatgttcccc gtgaagaacc
      901 ggctgaaatc ccggagactt tggtgaagca ggcggttttt gcgagaatcc ggatgaaggc
      961 cggcaggacc aggagtgagg ccagcccggt cgtgatcatc gtgatcccga tcaggatccc
     1021 gaaattgatc aggggaacaa aactggagaa cgccaggaca aagaatccgg ccatcacggc
     1081 aaccatgttg aagagaatcg cccttcccgt ctgcatcccg gtcctgtgca tcgcctcgtc
     1141 aagactcatc ccgcgccgga cccggaccag catcccgttg atgaaatgaa tcgaataatc
     1201 gacaccaata ccgatggcga tactggcaat catggaagtc ccgacatcca gcaggagtcc
     1261 gaacgccccc atgattccga aattgacggc aatcgccatc gagaggggaa ccagactgac
     1321 agcagcccag aagatggacc ggaacacaac ggcattcagg aggaatacgg caatgatcga
     1381 gagaaggatg ctcacaatct gcccgaggac gatcagtctg tcaacctgga gatacttggc
     1441 catcacaccc aggaattcga aatccggcag attgttcgcg gccatatact taccgatgga
     1501 tgctttgagc tccccggcca gcacggtcga gccatccttc attgtgacaa caacccgcag
     1561 tgtcttcccg tcacggctga caagatcgtc tgttgcaccg cgaaggatga gcatgtaatc
     1621 catcacggcc ctgcgggaat cagggatccg gtaccagtcc tttttttctc catgcaggga
     1681 gcggttggcc ttcttgatgt aatccgccaa gtggagggca ccgccaaaat tggatcgcac
     1741 ctctgccgga accgccgcat tggtcatcag ccagtcttca aggccatcca caatccggag
     1801 gagtttcggt tcaaaggcac cgttttctgt gtccgtatgg atcaggatct cggctgtcga
     1861 tgtgcccttg aagtgggtat tgatgaaagc gtcagccttg cggatttcgg tatcactctg
     1921 gaacatccgc acggcattgg tatccaccct gatcgatccc gcaaaaattc cggagatgac
     1981 aacgaagagg acccctacgg taaagaccgg ccagacatgg cgttttgaga caccggagag
     2041 catccccaga atcttcccgt cggcactctc ttcatgggtc tcctgcatcg gtttcatcct
     2101 catcaggatg gccagtgccg gaacgaagag gactgccaga atgaaggctg ccaaaacgcc
     2161 gatggccgtg aagatcccga agtcccggat gggaatgatg gaattgctcg ccaatgaggc
     2221 aaaccccccg acggttgtaa aggcggccat cagaacgggg acgaacgtct cagtcacgga
     2281 ttcccggaca gctttgtgca tgtcgctgcc gccatgcagg agataggcgt tgaccacatg
     2341 gatggcgtag gcactgccca cggcaatcat cagtaccggg agaacaacgg agatcaccgt
     2401 gatagacgaa ccggtcatcg ccatcaatcc gatggtgatc acggttgcat acaggacgcc
     2461 gatcagggga aggatcgtgg cgcccaacct gcggaaggaa accacaagac tggtgagcac
     2521 cacgatcagg acgaagggaa agaggatctc cagatcacgg accatgaatt tcccgaggag
     2581 tgtggtcacc accggttcgc cggcgatgaa gacatccgca tcggcaaacc cggcagccgt
     2641 aacctctttt ttcacatact cgagcgctgt ttcggaaaca ctctgtttga agctgcgcaa
     2701 tctgacgaga atcgaagcca cggtatggtc atc
//
LOCUS       DAOSHE010000283         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35696_length_2729_cov_2.964099, whole genome shotgun sequence.
ACCESSION   DAOSHE010000283 DAOSHE010000000
VERSION     DAOSHE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35696_length_2729_cov_2.964099"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLD82_07730"
     CDS             <1..348
                     /locus_tag="PLD82_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65308.1"
                     /translation="RTADCMIHLGLFDEAEKILSEILSHSWDRYAVYGMARIQRSTNR
                     TEMAIQNYKALLIEDPSDIRTIINFAELLAATEGYERAMAFLEGKRHELHGLRDLEPA
                     IKRFSSNQLGAQA"
     gene            complement(455..1738)
                     /locus_tag="PLD82_07735"
     CDS             complement(455..1738)
                     /locus_tag="PLD82_07735"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG65309.1"
                     /translation="MDIRIGIAGLGTIGSGVVELVRANSTVIREKTGISLRVAAIADP
                     AIDSRPDFDRSGIRICKDGMDLVTDPEIDIIVELVGGVAFPKKLVLAALENGKHVVTA
                     NKALLAKEGQEIYKTAAANRLALGIEASVAGGIPVIKTIRESLVGNRILEIRGIINGT
                     CNYILTRMAEHGLGFADALAEAQSRGFAEADPTLDITGGDAAHKIAILAALAFNTPVD
                     MADVHIEGIDRIDTNDIRYARELGYVVKLIGIARDRGAEGIEARVHPTLVPVDDQLAS
                     IRNEFNAVFLRCDFLGDGMLTGKGAGSRPTASAVVGDIVDIALAMRDHESMPPFFASE
                     RSRPVVPFGRTENRYYMHFSTVDKPGIMSRITGILGENNISIASMIQKEAGSEEAVPV
                     VMISHTAVEENMARAIKAIDALPEVRKPTVVIRAL"
     gene            complement(1766..>2729)
                     /locus_tag="PLD82_07740"
     CDS             complement(1766..>2729)
                     /locus_tag="PLD82_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65310.1"
                     /translation="SLPLAADWYPSDPFSPGVKALSVGGAFTALADDASAVHANPAGL
                     VQMDKAVFSWQLLSQIQMQRLVDTSIKLDWEYFPLVAACFPIEDGLVHGALSWTTQWR
                     SISGRYSVHVLGASLSWKISSWLSWGNTLGLAVGLTDDEAASGFFWQTGFLVPLNEDI
                     KLGLTMRMPVTLEWDVLRGDTGVRERLPLSMQAGMAWRIDRHAILSFDLEFVDVAGLR
                     YSSDAGTMNPVQPTGLFRNLHPHIGFQTLIPSIGAEFRCGFLTFTHNANNASDAQPAL
                     TLGIGAWTSQYFRIDFALQDTLIFDIFTRTNRYERISVSFEYVM"
BASE COUNT          620 a          815 c          762 g          532 t
ORIGIN      
        1 aggacggcag actgcatgat ccatctcggg ctcttcgacg aagccgagaa gattctttcg
       61 gagattctca gccactcatg ggaccgctat gccgtctatg gcatggcccg gatccagcga
      121 tcgaccaacc ggacggaaat ggccatccag aattacaagg ctctcctgat cgaggatccg
      181 tccgacatcc ggaccatcat caattttgcc gaactcctcg ccgcaaccga gggctacgaa
      241 cgagccatgg cgtttcttga aggcaaacgc cacgagctgc acggcctgcg ggatcttgaa
      301 ccggccatca aacgcttctc ttcgaatcaa ctcggcgctc aagcctgatc ctccggatat
      361 gacgaagcgc agatctggcc aaagtgcggc tcgcgttctc agatgcaaaa agacctgcgt
      421 gtgaccgcag gtctttttgt ggaacaggaa cccgttacag tgcccggatg accaccgtcg
      481 gcttgcggac ctcgggcagg gcgtcgatcg ccttgatcgc ccgcgccata ttctcctcga
      541 cagcggtgtg actgatcatc acaacgggaa cagcttcctc gcttcccgct tctttctgga
      601 tcatgctggc aatactgata ttgttctcgc ccaagattcc ggtgatcctg gacatgatcc
      661 ccggtttgtc aaccgtggaa aaatgcatgt agtaccggtt ttcggtccgt ccgaagggga
      721 caacgggacg ggaacgctcg gaggcgaaaa agggcggcat gctctcatgg tcccgcatgg
      781 ccagggcaat atcgacaata tcccccacaa ccgctgaagc ggtcgggcgg gagccggcac
      841 ccttcccggt cagcatcccg tcaccaagaa aatcacagcg gagaaaaacg gcattgaact
      901 cgttgcggat cgaggcgagc tggtcgtcaa ccggaacgag ggtcggatgg acacgggcct
      961 cgatcccctc ggcaccacga tcccgggcaa tcccgatcag tttgaccaca tacccgagct
     1021 ctctggcata gcggatgtca ttggtatcga tccggtcgat cccctcgatg tgtacatccg
     1081 ccatgtccac cggggtattg aaggcaagcg cagccaggat ggcaatcttg tgggcggcat
     1141 caccacccgt gatgtccaga gtcggatcgg cttcggcaaa cccgcgggac tgggcctctg
     1201 ccagagcatc agcaaacccc aatccgtgtt cggccatccg ggtcagaatg taattgcacg
     1261 tcccgttgat gattccgcgg atctcgagga tcctgtttcc gacaagagat tcgcggatgg
     1321 tcttgatgac cggaatcccg ccggccacac tggcctcgat cccgagcgcc agcctgttcg
     1381 cggccgcggt cttgtagatc tcctgccctt ccttggccag aagagccttg ttggcggtca
     1441 ccacatgctt gccgttttcg agagcggcca gaacgagttt tttggggaaa gcgacaccgc
     1501 ccaccagttc gacgatgatg tcgatctccg gatcggtcac aagatccatc ccgtccttgc
     1561 agatccgaat cccgctccgg tcaaaatccg gccgggaatc aatggccgga tcggcaatcg
     1621 ctgcgactcg cagggagatc cccgtctttt cgcggatcac tgtggaattg gcccgtacaa
     1681 gttcaacaac accggagcct atggtgccaa gtccggcaat gccgattctg atatccatgc
     1741 tgtttctccc tgatctcgtg tcgtgctaca tgacatattc aaacgaaacg gaaatccgct
     1801 cgtatctgtt ggttctggta aaaatatcaa aaatcagcgt atcctgcagt gcaaaatcga
     1861 tcctgaagta ttggctggtc catgcaccga ttccgagggt cagggccggt tgcgcatcgg
     1921 atgcgttgtt ggcattatgg gtaaaggtca aaaacccgca ccggaactcc gcaccgatgg
     1981 acgggatcaa ggtctgaaac ccgatgtgcg gatgaagatt gcggaagagt ccggtcggtt
     2041 ggaccgggtt catggttccg gcatcgctcg aataccggag ccctgcaaca tcgacgaatt
     2101 cgagatcaaa cgagagaatg gcatgccggt cgatccgcca ggccatgccg gcctgcatcg
     2161 ataagggcag ccgctcgcgc acaccggtat cccctcggag cacatcccat tcgagagtga
     2221 cgggcatcct catggtaagg cccagcttga tatcctcatt gagcggaacg aggaaacccg
     2281 tctgccagaa aaacccggac gcggcttcgt catccgtcaa accgaccgca agaccaagcg
     2341 tattccccca ggaaagccag gacgaaatct tccaggaaag actggccccg aggacgtgga
     2401 cgctgtagcg accggagatg ctccgccact gagtcgtcca ggagagtgct ccatgcacca
     2461 agccatcctc gatagggaag caggccgcta caagcggaaa atactcccag tcgagtttga
     2521 tgcttgtatc aaccagacgc tgcatctgga tctgtgaaag caactgccac gaaaagacgg
     2581 ccttgtccat ctgtaccagc ccggcgggat tggcatggac cgcactggcg tcatcggcca
     2641 gagccgtaaa ggcaccaccc accgagagcg cctttacacc cggcgagaac ggatcggatg
     2701 gataccagtc agcggcaaga gggagggaa
//
LOCUS       DAOSHE010000284         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35944_length_2715_cov_4.340977, whole genome shotgun sequence.
ACCESSION   DAOSHE010000284 DAOSHE010000000
VERSION     DAOSHE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35944_length_2715_cov_4.340977"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLD82_07745"
     CDS             <1..1148
                     /locus_tag="PLD82_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65311.1"
                     /translation="ASRNRVLENFNTGIQAFKKREYDKAIETWKQIDGMPDATESDRQ
                     MVQGYIKTAEEVRVYNQNRYFILGEESAKAGRLLQAREALEEALKINKYHEKARTLLA
                     DVKDRITAYAFAQLRRGESLYETGNYTNAVTVLESAKLHNESDDRIQNKLDEAGDNLK
                     LLQEAITLEQQGKPADAIVLYQKIVQNNPKDQNTRTRINGLSRRLSEETDQMIKKAEK
                     QIAEKMYNDALLGLELVLQIVQYKQSSQARETTTRYARQLQGQARDLMAKALADAWAQ
                     GMRLYQAGNFEGSLPFFEQVWIYDRNYREIRTVRRVAMNKVAEARAAVAKRNEAQIQQ
                     LLYSGMSFYQQGNYQGAINAWSQILAISPGHSAAVSYIARARFKLGQ"
     gene            1172..>2715
                     /locus_tag="PLD82_07750"
     CDS             1172..>2715
                     /locus_tag="PLD82_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65312.1"
                     /translation="MKGYGVRRTVLIMLGVTLLGCLGAARALFAAPPVDLVRNFPRAE
                     FFQRSGYVKDIAVLGDVVWYATANGLVSYDVGRDAWGVFHDGNSPLPANYVSKLAVDQ
                     GEGSLWMATAQGVARYRPAEQTPGLRWKVYTKRDGLADEETTSVAVDEEFVWVGTRYW
                     GVIRFDKRQQIWRSAPYTPIDGLGANRIHALAVDGDQIWAGTDDGLSVYDRYADLWTT
                     YDASQGLTAKQVTCVDSDGKNVWVGTYGGGLLRFDKDSMRFQAYRVRDGLADDTVFAV
                     RSDGEHVWAGTFGGVSRLDRSAGGSEWTTITKASHGLLDNSVSAIALYGSQLFLGTDG
                     EGGMMLDKKTPQVWVHPKTGYEKPGSILIQAGLRSTQPVVTAGASWRPSIYVTQPYST
                     AGLVLLGQEKIRGARSLGALDVARLDAGNLKEGLTYDLVFEITDASGFKNRAVWPLVI
                     DATKPVITVDDAPETVKTREYTLRGRYLELYPREFEYAQNGGSFTRLQEVDRKMRTWK
                     QTLNLVP"
BASE COUNT          620 a          753 c          839 g          503 t
ORIGIN      
        1 cggcttcccg caaccgtgtt ctcgagaact tcaataccgg gatccaggct ttcaaaaaac
       61 gggagtacga caaggcgatc gaaacctgga aacagatcga cggcatgccg gatgcaaccg
      121 agagcgaccg gcagatggtc caggggtaca tcaagacggc cgaggaagtc cgcgtgtaca
      181 accagaaccg gtacttcatc cttggcgaag agtccgccaa agccggtcgt ctcctccagg
      241 cacgcgaggc cctcgaagag gccctcaaga tcaacaagta tcacgagaag gcccggacac
      301 tcctggcgga tgtcaaggac aggatcacag cctatgcctt cgcccagttg cgtcgtggag
      361 agtcactcta cgagaccggg aattatacaa atgcggtcac cgttctcgaa agtgcaaaac
      421 tgcacaacga gagtgacgac aggatccaga acaaactgga cgaagccggc gacaacctca
      481 agctcctgca agaggcgatc accctcgagc aacaggggaa accggccgat gcgattgtcc
      541 tctaccagaa aatcgtgcaa aacaatccca aggaccagaa taccaggacc aggatcaatg
      601 gcctctcgag gagactctcg gaagagacgg accagatgat caaaaaggca gagaaacaga
      661 ttgccgaaaa gatgtacaac gacgccctgc tcggactcga actcgtgttg cagattgtcc
      721 agtacaaaca gagttcccag gcacgggaaa cgacaacacg ctatgccaga cagcttcaag
      781 gtcaggccag ggacttgatg gccaaggccc tggctgatgc ctgggcgcag gggatgcggt
      841 tgtatcaggc cggaaatttt gaaggatcac tcccgttttt cgaacaggtc tggatctacg
      901 acaggaatta ccgcgagatc aggacagtcc ggcgcgtggc gatgaacaag gtggccgagg
      961 cccgggcggc cgtggccaaa cggaatgaag cccagatcca gcagcttctg tacagcggca
     1021 tgagcttcta ccagcagggc aattatcagg gggccatcaa tgcctggtcc cagattctgg
     1081 cgatctcccc ggggcattca gctgccgtga gctatatcgc ccgggcccgt ttcaagttgg
     1141 gccagtgagg gatgcgggga gaaaggattc catgaagggg tatggcgtgc gcaggacggt
     1201 tttgatcatg ctgggagtga cgctgctggg atgccttggt gccgcccggg cgctttttgc
     1261 cgccccaccg gtggatctcg tcaggaattt tccccgggcg gaattttttc aacgttccgg
     1321 ctacgtcaag gatattgccg ttcttggcga tgtcgtctgg tatgcgacgg ccaacggact
     1381 cgtctcctat gatgtcggtc gtgacgcctg gggtgtgttt cacgatggaa actccccgct
     1441 tcccgccaac tatgtctcaa aactggctgt cgatcagggt gaggggagtc tctggatggc
     1501 cacagcccag ggtgtggcca ggtatcgtcc agctgaacag actcctggcc tgcgctggaa
     1561 ggtctataca aaacgggatg gcctggctga cgaggagaca acctcggtcg ccgtcgatga
     1621 ggagtttgtc tgggtcggaa cccgctactg gggtgtgata cgttttgaca agcgacaaca
     1681 gatctggaga agcgccccct acacaccgat tgacggcttg ggggccaaca ggatccatgc
     1741 tcttgccgtt gacggtgacc agatctgggc cgggacggac gacggcctct cggtctatga
     1801 ccgctatgcc gatctctgga cgacctatga tgcatcccag ggcttgacgg ccaaacaggt
     1861 gacctgtgtg gacagtgacg ggaagaatgt ctgggtaggg acctacggcg gcggactgct
     1921 ccgttttgac aaggacagca tgcgtttcca ggcctaccgg gtccgggatg gtctggccga
     1981 cgatacggtg tttgccgtgc gatcggacgg agagcatgtc tgggccggga cctttggcgg
     2041 ggtctcccgt ctcgaccgca gtgccggtgg aagcgagtgg accaccatca ccaaggccag
     2101 ccatgggttg ctggacaatt ccgtcagtgc gattgccctg tacggaagcc agctgttttt
     2161 ggggacggac ggcgagggcg ggatgatgct cgacaagaaa accccccagg tctgggtcca
     2221 tccaaaaacc ggctacgaaa agcctgggag catccttatc caggccggtc tgcgcagtac
     2281 ccagccggtg gtgacggccg gtgcctcctg gcgaccatcc atctacgtga cgcaacccta
     2341 ttcgacagcc ggactggtct tgctcggtca ggagaaaatc cgcggggcca ggagtctggg
     2401 ggctctcgac gtggcgcggc tggatgcggg aaacctgaag gaaggcttga catacgatct
     2461 tgtcttcgaa attaccgacg cctccggttt caaaaacagg gctgtctggc ccctggtgat
     2521 cgacgcaacc aaaccggtca tcacggtcga cgatgctccc gaaaccgtca agacacggga
     2581 gtataccctg cggggacgct atcttgaact ctatccccgg gagttcgagt atgcacagaa
     2641 cgggggaagt ttcacccggc ttcaggaggt cgaccgcaag atgcggacct ggaagcagac
     2701 cctgaacctc gttcc
//
LOCUS       DAOSHE010000285         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_35990_length_2713_cov_3.019940, whole genome shotgun sequence.
ACCESSION   DAOSHE010000285 DAOSHE010000000
VERSION     DAOSHE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35990_length_2713_cov_3.019940"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLD82_07755"
     CDS             complement(<1..269)
                     /locus_tag="PLD82_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65313.1"
                     /translation="MMDRLCTRITESARINGRHFIWRTLAASVILLASVAAGLGLVAM
                     SEMAIPFEGNLHIAGIFALIGALIAVVMVILMRAMILRRLIGGLDR"
     gene            complement(266..1234)
                     /locus_tag="PLD82_07760"
     CDS             complement(266..1234)
                     /locus_tag="PLD82_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65314.1"
                     /translation="MRSDSPGSVRRSHGTARQTLVLLLAPLVALVAWWAVLCFSLNAI
                     VFPPAWLLVLFLVPPLALACVLLVVSRAVVFGFYGGVTRRIRQTAGLEGDEGDATPFF
                     LFHSGQELEMLTGVLASWKQEVAVSRDRLAGVAGLWSLEPQARFPWAGRSRVYQLKSN
                     ELKKAKGYTQVRRLACLAVFIAPPGEETGIRKRFEWMAEVSGSIAGMAEENGGFVALV
                     MGRVLLLVFSLGSDERTLAIRAVKTARNIQELLAAEFPGSTVRMAGDIFQAVESMVQV
                     PAGLRYHIEAPEIAALRLAAGNAGFEGGLRLTSRLVASIKTGEAGA"
     gene            1419..>2713
                     /gene="aspS"
                     /locus_tag="PLD82_07765"
     CDS             1419..>2713
                     /gene="aspS"
                     /locus_tag="PLD82_07765"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG65315.1"
                     /translation="MYRNEYCGTLTKKDVGREVELAGWVLRRRDHGGVIFVDLRDRSG
                     YSQIVFNPDASSDAHAQAEHLHNEYVIRVRGKVVDRQENINPKIPTGEIEVIASSLEI
                     LSTSETPPFQLDGHASVNDELRLKYRFLDLRQPEMLENMVRRHQLAQAAREVLNTEGF
                     LEIETPILNKSTPEGARDFLVPCRLQPGSFYALPQSPQIFKQILMVAGMDRYYQIVKC
                     FRDEDLRADRQPEFTQIDIEMSFIHQEDILALVEKLIAHVIGKVYGKTVPTPFPRLDY
                     RDAMARYGSDKPDTRFGLELVEITDIGKASSFKVFQSLAESGGIIKGFNAKGGAKFSR
                     KDIEEEFTGYAGVFGAKGLAWMKVSEAGLESNIVKFFSEEQQAELKKRMQAEPGDLLL
                     FVADKPKVTNDALANLRIKLGHKLGLIDPEAMNLLWVVD"
BASE COUNT          606 a          898 c          737 g          472 t
ORIGIN      
        1 cggtccaacc cgccgatcaa cctcctgagg atcatggccc gcatgaggat gaccatcacg
       61 acggcgatca gggctccgat caaggcaaaa atcccggcga tgtgcagatt cccttcgaaa
      121 ggaatcgcca tttcggacat cgccaccagc ccgagtccag cggccacact ggcaagcagg
      181 atcaccgagg ctgccagcgt gcgccagata aaatgccgac cgttgatcct ggccgattca
      241 gtaatgcggg tacacaatct gtccatcatg ctcccgcctc cccggtcttg atactggcca
      301 cgagtctcga ggtgagcctg agccctccct cgaacccggc atttcccgca gcaagccgca
      361 gagccgcaat ctcgggagcc tcgatatggt agcgaagccc ggcgggaacc tgaaccatgc
      421 tctcgaccgc ctgaaagatg tcccccgcca tccggaccgt gctgcccgga aactcggcag
      481 caagcagctc ctggatgttg cgcgccgtct tgacggcgcg gatggccaga gtccgttcat
      541 cactccccag ggaaaacacg agcaggagaa cccgccccat gacgagggca acgaatccac
      601 cgttctcctc ggccatcccg gcaatactcc cggagacctc ggccatccac tcaaaccgct
      661 tccggatgcc tgtttcctct ccggggggtg caatgaagac ggcaagacac gcaagccgcc
      721 ggacttgagt gtaccctttg gcttttttca gttcgttcga tttgagctgg tagacccgtg
      781 accgaccagc ccagggaaac cgggcctgcg gctcaagcga ccagagcccc gcaacacccg
      841 cgagccggtc acgggaaaca gcgacctcct gcttccagga tgcaagcacc cccgtcaaca
      901 tctcgagctc ctgcccggag tggaagagga aaaatggagt cgcgtctccc tcgtctcctt
      961 cgagtcccgc tgtctgccgg attctccggg tcaccccgcc ataaaacccg aatacaaccg
     1021 cacgggaaac gaccagcaaa acacaggcca gcgcaagggg cggcacaaga aagagcacga
     1081 gaagccaggc gggtggaaaa acaatcgcgt tcagggaaaa acagaggaca gcccaccacg
     1141 ccaccagcgc gacgaggggc gccaacagga gcaccagggt ctgacgggcc gtaccatggg
     1201 atcgcctgac gctccccggg gaatcactcc tcatgctcgc tctccactca accggactga
     1261 tgcgggtccc tgcaatccgg atggcccgct ctccccagtc tatccccgca tggaccagcc
     1321 tgtcaaagga tacataaatt ggtattcccc gctctgcgag tgctattttt tcccgacaag
     1381 actccgagaa atcaacaatt tggaaggaat cccccacgat gtacaggaat gaatactgtg
     1441 gtacgctcac aaaaaaagac gttggccgcg aggtcgaact tgccggctgg gttctgcgcc
     1501 gacgtgatca cggtggtgtg atctttgtcg acctcaggga ccgctctgga tactcccaaa
     1561 ttgtcttcaa cccggatgca tccagcgacg cccatgcgca ggccgagcat ctgcacaacg
     1621 agtatgtgat ccgcgtccgc ggaaaagtcg tcgaccgcca ggagaatatc aatccgaaga
     1681 ttccgaccgg cgagatcgag gtgatcgcct ccagtctgga aatcctctcc accagcgaaa
     1741 ccccgccctt ccagcttgac ggacacgcct cggtcaacga tgagctccgc ctcaagtacc
     1801 gcttcctcga cctgcgtcag cccgagatgc tcgagaacat ggtccgccgg caccagttgg
     1861 cccaggccgc cagagaggtt ctcaacaccg agggcttcct cgaaatcgag acgccgatcc
     1921 tcaacaagtc cactcccgaa ggcgcacgcg acttcctcgt cccctgccgc ctccagccgg
     1981 gctccttcta tgccctcccc cagtcacccc agatcttcaa gcagatcctg atggtggccg
     2041 gcatggaccg ctactaccag atcgtcaaat gcttccgcga tgaggatctg cgggccgatc
     2101 gccagcccga gttcacccag atcgatatcg aaatgtcttt cattcaccaa gaggacatct
     2161 tggcgctggt tgagaagctg atcgcccatg ttatcggcaa ggtctacggc aagaccgtcc
     2221 cgaccccttt cccgcggctg gactaccgcg atgcgatggc ccgctatggt tcggacaaac
     2281 cggacacccg gttcggcctt gagctggtcg agatcaccga catcggcaaa gcttcgtcgt
     2341 tcaaggtctt ccagtccctg gcggagtccg gcggcatcat caagggcttc aacgctaaag
     2401 gcggtgccaa attctcgcgc aaggacattg aagaagagtt taccgggtac gccggggtct
     2461 ttggcgccaa gggcctggcc tggatgaagg tcagcgaagc gggtctcgaa tccaatattg
     2521 tcaagttctt ctcggaggag cagcaggccg agctcaaaaa gcggatgcag gccgagccgg
     2581 gcgacctcct gctctttgtc gccgacaaac cgaaggtcac caacgacgcg ctggccaacc
     2641 tgcgcatcaa actcggacac aagctcggcc tgatcgaccc ggaggcgatg aacctcctct
     2701 gggtggtcga ttt
//
LOCUS       DAOSHE010000286         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36288_length_2697_cov_4.305450, whole genome shotgun sequence.
ACCESSION   DAOSHE010000286 DAOSHE010000000
VERSION     DAOSHE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36288_length_2697_cov_4.305450"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..1371
                     /locus_tag="PLD82_07770"
     CDS             340..1371
                     /locus_tag="PLD82_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG65316.1"
                     /translation="MLTSIHFEQFTAFSKLDLKLSSGINIFIGENGTGKTHILKALYA
                     ACDVSKSRKGFADKINDIFSPAGKQIGRLVKRSAVSSSGSVEVRRRPNGKEIALKLTI
                     SNHTLTPDKARVSGSTVQWAEQPVESVYIPVKDMMANAPGFRSLYATRNIHFEEVYAD
                     VIDRAFLPLRKGPMDQDRARILKILQKSMDGKVIAKNEEFYLKSTSGMLEFTLLAEGF
                     RKLGLLWLLIQNGTLLSGSVLFWDEPETNLNPRLMQTVVTIMVELQRLGVQICIATHD
                     YVLLKEFDLAVQSDDRIRYHSLYRDAKTGTIVCAGSDSLQELAPNVIDDTFGYLVDRQ
                     VSRDMGGAG"
     gene            1372..1890
                     /locus_tag="PLD82_07775"
     CDS             1372..1890
                     /locus_tag="PLD82_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65317.1"
                     /translation="MRVVSDGFQIDFRGAIEAFKFDDTDPASPHFHKAPMKAVDIIAE
                     FKNEYVFVEIKTQYFPEDAEEIDRLTSAGQLPGQPAYFKWLKEYLKYKFRDSLLYRLA
                     EGKLDKPVRYICLLNVEDGLILKIKSDLKKELPVGLAGPRWKTGLAISCHVHNLASWR
                     RNHPGWPVRICR"
     gene            complement(1880..2092)
                     /locus_tag="PLD82_07780"
                     /pseudo
     CDS             complement(1880..2092)
                     /locus_tag="PLD82_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
BASE COUNT          702 a          601 c          730 g          664 t
ORIGIN      
        1 ccaaagcgga aaaaaattcc acatgccact gcacagagcg tacaccctgg ctcgtctacc
       61 tcaaagagaa ttattcctgg gacaacgggg gaggggtgca attgcgtttt tgtgatattg
      121 ttatgcgaat ccgagatacg tatgatggga ttataaaacg tcattagtat cccggataca
      181 ggagtgttgc ggtacaatgg acaaaatggt ttcatcgcga aatgcgttgt acacagtttc
      241 aaaaatcagg tatactacag gcagcaaacc cgccatgcct ctgcgactgc acgcacactc
      301 tggtgggtct ttcttgtggt actgcacggg gagatcgaaa tgctgaccag catacatttt
      361 gagcagttta ccgctttttc caagctcgac ctgaaacttt cttcggggat taatattttt
      421 attggagaaa acggaactgg gaaaacccat attctcaagg ctttgtatgc cgcctgcgat
      481 gtttcaaaaa gccgcaaggg gtttgctgac aaaatcaacg atattttttc acctgccgga
      541 aagcagatag ggcggttggt caaacgttct gctgtcagta gttcggggag tgttgaagtt
      601 cggcgccggc caaatggtaa agagatcgca cttaagctga ccatcagtaa tcatacactg
      661 acacctgaca aggcaagagt gtcaggttca acggtccaat gggctgagca acccgtcgaa
      721 tcggtctata ttccggtcaa ggacatgatg gccaatgcac ctggcttccg atcgttgtac
      781 gcaacgcgaa atattcattt cgaagaagtg tatgctgatg tcattgatcg tgccttcctc
      841 cccctgagaa aagggccgat ggaccaggat cgggcaagga ttctcaagat tttgcaaaag
      901 tcaatggatg gaaaggtcat tgcaaagaat gaagagtttt acctgaagag cacgtcaggg
      961 atgctcgagt ttaccttgtt ggctgaaggc tttcgcaaac tgggcctgct ttggttgctg
     1021 atacaaaacg ggacactttt atcagggagc gtgctctttt gggatgagcc tgaaacaaac
     1081 ctcaatccca gattaatgca aactgtggtc accatcatgg ttgagctgca gcggcttgga
     1141 gtgcagatat gtattgcaac ccatgattat gtcctcctca aagaatttga tctcgcggtt
     1201 cagtccgatg atcgtatccg ctatcatagc ctgtatcgtg atgcaaagac cgggacgatt
     1261 gtctgtgcag ggagtgattc cttgcaggaa cttgcaccca atgtgataga cgataccttt
     1321 ggttatcttg ttgaccgtca ggtttcaagg gatatggggg gggctgggta aatgagggtt
     1381 gtgtctgacg ggtttcagat tgatttcagg ggtgcaattg aggcattcaa gtttgatgat
     1441 acagatccgg caagtcctca tttccacaag gcaccgatga aggctgtgga tatcatcgcc
     1501 gagttcaaaa atgagtatgt ctttgtcgaa atcaagacgc agtactttcc cgaagatgca
     1561 gaggagatcg accgattgac cagtgctggt caactgccgg gacagcctgc atatttcaaa
     1621 tggttgaagg agtatctgaa atacaaattt cgtgattctt tgctgtaccg gcttgcagaa
     1681 ggcaaacttg acaaacccgt cagatacatt tgtctgctca atgtggagga tggcctgatt
     1741 ttgaaaatca aaagtgatct caaaaaggaa ctgcctgtcg gtttggctgg acctcgttgg
     1801 aagaccggtc tcgccataag ttgccacgtg cacaatctgg cttcatggag gcgcaaccat
     1861 cctggttggc cagtacgtat atgtaggtga tcgtctcgat gcccctgtgc gggtgcatgg
     1921 gaaaaccgcg ttcgtacagg gcaggatcat ccccgcgaaa gtcatcaagc agcaaaaagg
     1981 ggtcaaagcg ggacagttcg gcgtggccga agacccgcat caggtgcacg cccgcgcctt
     2041 cggtgactgg ctgggccttg aggacatcgc tgactggtcg atacgaggac attggcgcca
     2101 tcctgtgtct gtttgatacc gggttgtatg gtgatgcccg gcaatgcttc caaagcggaa
     2161 aaaaattcca catgccactg cacagagcgt acaccctggc tcgtatcacg tacagtgaag
     2221 gatacctcga ttatgtgagc aatcagaaaa aaagtgaaaa aggagtgaac aaggacaccc
     2281 cgaaaaatcc tatatcatga tgcaggctca ggttgttttc ggaacgggga tccagcttta
     2341 gagtgcctgt tgatggaacc attggaaacg cctatgtccg cagtatccgg gattgcctgc
     2401 gtatcaatgc aaacaggaga tcgtatggcg gatgtgtgca tctattgtgg atcgtcagca
     2461 tatggaccct gcgaaaagag tccgcatgga gtccatgagc atacgggggg cgtgtatagc
     2521 tgtgtctttt gtggctcccg ggcctatgga gtctgtctgc tcagtcccca tgggcatcac
     2581 cagcacgggc atgagtatca tcgctgcatc tattgcgaat cggaacatca ggggccgtgt
     2641 cgctacagcc cttcaggata tcacgaacaa tgaagacaag ggatacagca atgcaag
//
LOCUS       DAOSHE010000287         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36340_length_2695_cov_3.002652, whole genome shotgun sequence.
ACCESSION   DAOSHE010000287 DAOSHE010000000
VERSION     DAOSHE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36340_length_2695_cov_3.002652"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /gene="gpmI"
                     /locus_tag="PLD82_07785"
     CDS             <1..1415
                     /gene="gpmI"
                     /locus_tag="PLD82_07785"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG65318.1"
                     /translation="PSNQGSSEVGHLNMGAGRVVYQSLVRISREIDLGTFYKNAVLID
                     AMQRVKGTGKKLHLWGLVQDQGVHAHSEHLIALLRLAGQLGLAREQVLVHVFTDGRDT
                     PPQSAGKYVAVVEAAMRELGMGVFASICGRYYAMDRDNRWERVKLAYDLLVSGTADGK
                     AETIQSAIEAAYTAGENDEFIKPRILPGFAPVSDGDSVIFFNYRLDRTRELTKAFVQD
                     GFAEFPVKAIRDLSYVCFTEYYEGVVNSTRAAVSVAFGPTDFANLFGEFIAKQGLRQL
                     RIAETEKYAHVTFFFNGQSDVVFENEDRVVIPSPQVATYDLKPEMSAYEVRDKVLELL
                     DKDIYDVVILNFANPDMVGHTGIFEAAVKACEAVDECVGAVVDKILELDGVALLTADH
                     GNAEQMRDYITGEPMTAHTSNLVWCTLISNRPGLQNGVIRLKPSGGRLADTTPTLLDI
                     MGYQKPAGMDGESLIERIGK"
     gene            1412..1741
                     /locus_tag="PLD82_07790"
     CDS             1412..1741
                     /locus_tag="PLD82_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65319.1"
                     /translation="MSVRGRYGWILLTGLAMAGGLWIRVSGAFEAWFEPWGWGLALFL
                     TGHGAGRILRSLRKDPLPLWLAILVLGALPAVLAAPDRGGLALAGAGLAWACGLGLVG
                     FPAGFDV"
     gene            1872..>2695
                     /locus_tag="PLD82_07795"
     CDS             1872..>2695
                     /locus_tag="PLD82_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOG65320.1"
                     /translation="MGNSIAFSRDGRHLASGSSEHHIRIHEIIDRSAQLCGRYGDHAD
                     QVRSLCFSHGGDVLASGSLDTSIRIWDFANGNLKPVQVLEGHTDTVFCLAFSPDDEYL
                     ASGGLDNTLRLWRRTGDGYMPVQVLSEHTSWVNDVVFHPEEQMLFSCGSDNVIRVWEL
                     DENRFVERQAIKAHSAWVNALAVSADGTILASGGSDSCVRIWHEVNGIWREKTTLRGY
                     AEGVFSLAFDPQGRYLATGSKDHWVKIWERVDEDFSLENTLSGHTQCVFSLAFEPHG"
BASE COUNT          494 a          718 c          888 g          595 t
ORIGIN      
        1 ccccgtccaa tcagggttca agcgaggtgg ggcacctgaa tatgggggcc ggacgcgtgg
       61 tgtaccagtc gctcgtccgc atcagccggg aaatagatct tggtacgttc tataaaaatg
      121 cggtcttgat cgatgccatg cagcgtgtca agggaaccgg aaaaaagctc cacctctggg
      181 ggcttgtgca ggaccagggc gtacatgccc acagcgaaca tctgatcgcc ctgctccggt
      241 tggcggggca gcttgggctg gcccgtgaac aggtcctggt gcacgtcttc acggatggac
      301 gggatactcc gccccagtcg gccggcaaat atgtggcggt tgtcgaggcg gcgatgcggg
      361 aactcgggat gggcgtcttt gcctcgatct gcggccggta ttacgccatg gaccgggaca
      421 accgctggga gcgcgtcaag ctggcctatg acctgctcgt gtcggggaca gccgatggca
      481 aagccgagac gatccagtca gccatcgagg cggcgtatac agccggcgag aatgacgagt
      541 tcatcaagcc gcgtatcctg cccggatttg cgccggtctc cgacggggac agcgtgatct
      601 ttttcaacta cagactcgac aggacgcgcg agctgaccaa ggcttttgtg caggacgggt
      661 ttgccgagtt tcctgtcaag gccatcaggg atctctcgta cgtctgcttt acagagtatt
      721 acgaaggtgt tgtgaacagt acgcgggctg cggtatcggt ggctttcggc ccgacggatt
      781 ttgccaacct ctttggcgag ttcatcgcca agcagggact ccggcaactc cggatcgccg
      841 aaaccgagaa gtatgcccac gtcacgttct tcttcaatgg ccagtcggat gttgttttcg
      901 aaaacgagga tcgcgtggtg attccctcgc cccaggtcgc gacttacgat ctcaagcccg
      961 agatgtctgc ctatgaggtg cgtgacaagg tgctggaact cctcgacaag gacatctacg
     1021 atgtcgtcat cctcaacttc gccaacccgg acatggtcgg gcataccgga atcttcgagg
     1081 cggcggtcaa ggcctgcgag gccgtcgacg agtgtgtcgg tgcggttgtg gacaagattc
     1141 tcgaactgga tggtgtggct ctcctgaccg ccgaccacgg gaatgccgaa cagatgcggg
     1201 attacatcac gggcgagccg atgacggccc ataccagcaa tctggtctgg tgcaccctca
     1261 tctccaaccg acccgggctg caaaacgggg tgatccgcct caaaccctcc ggcgggcggc
     1321 tggcggacac gacgccgacc ctgctggata tcatggggta tcagaaaccg gccggcatgg
     1381 acggagagtc cctgatcgag cggatcggga aatgagtgtc cgtggccggt atggctggat
     1441 cctgctgacg ggcctggcca tggccggggg gctctggatt cgggtttccg gggcttttga
     1501 agcctggttt gaaccatggg gctgggggct ggcgctcttc ctgacaggcc acggtgccgg
     1561 gcggattctg cgcagtctgc ggaaagatcc tctccctctc tggctggcca ttctggttct
     1621 gggcgccttg cctgccgtcc tcgctgcacc ggaccgtggg gggctggctc ttgccggcgc
     1681 cggtctggcg tgggcttgcg gattgggttt ggtgggtttt ccggccggct ttgacgtgta
     1741 gactgcttca ggggttttct gtcgcgatgt gaccgtggac agggcgtgga ggctggtgta
     1801 tactctgcct tggtactgtg agtaccggca ggtcttggtg tgaactcgca aaacgggaaa
     1861 ggtaacgacc ggtgggaaac agcattgcat tcagcaggga tggcagacat ctcgcgtcgg
     1921 gctcaagcga gcaccatatc cgtattcatg aaatcatcga caggtcggcc cagctttgtg
     1981 gccgatacgg ggatcatgcc gatcaggtgc gatcgctctg tttttcgcat ggtggagacg
     2041 ttctggcttc gggcagtctt gataccagta tccggatctg ggattttgcc aacggcaatc
     2101 tcaagccggt ccaggtcctt gaagggcata cggatacggt tttttgcctg gcattcagtc
     2161 cggacgacga gtacttggca tctggtgggc tggacaatac cctccgtctc tggcgcagga
     2221 ctggtgatgg gtacatgccg gtgcaggttc tctccgagca tacgtcctgg gtgaatgatg
     2281 tggtttttca tcctgaggag cagatgctct tttcctgcgg ctcggacaat gtgatccggg
     2341 tctgggagct ggacgagaac aggtttgtcg agcggcaggc catcaaggcg cattcggcct
     2401 gggtcaatgc cttggcagtt tcggcggatg gcaccatact ggcgtcgggc ggttcggaca
     2461 gttgtgtccg gatctggcat gaggtcaacg ggatctggcg cgaaaaaaca accttgcgag
     2521 gctatgcgga gggtgtcttt tcattggcgt tcgatccgca gggcagatac ctggcgaccg
     2581 ggagcaagga ccattgggtg aaaatctggg agcgtgttga cgaggatttt tccctcgaaa
     2641 ataccctgtc cgggcatacc cagtgtgtgt tcagtctggc ttttgaaccg cacgg
//
LOCUS       DAOSHE010000288         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36437_length_2689_cov_3.299165, whole genome shotgun sequence.
ACCESSION   DAOSHE010000288 DAOSHE010000000
VERSION     DAOSHE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36437_length_2689_cov_3.299165"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLD82_07800"
     CDS             complement(<1..886)
                     /locus_tag="PLD82_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOG65321.1"
                     /translation="MSTDKILVPFNRPDIDEADIARVVATLRSGWITTGPAVREFESG
                     LRAYLGVSHVICLNSATAAMEMALLALGIGPGDEVVTTVYTYAATANIVRHTGARLVL
                     VDIGAEGFAIDPAAVEAAVNARTKAVISVDFAGIPCNYRALRTAIDSHKREFVASNEF
                     QASIGGAAILADAAHALGASFSGKRVPEWADMAAYSFHAVKNLTTAEGGALAFADLGR
                     GGGNHFGEQSSGPVSEGFERRVRLLALHGQSKDALAKMAAGSWEYDIEVSGYKHNMTD
                     IQAALGIGQLARYGARLER"
     gene            956..1528
                     /locus_tag="PLD82_07805"
     CDS             956..1528
                     /locus_tag="PLD82_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOG65322.1"
                     /translation="MSRYAIDARETPEPLSLPLVAIMAGSDILIQAVQERLVETYGPV
                     LAASEPFLFDNFTGYYKKEFGEGMHKQVCIFRTLREQRLLPDDKHASCAIERDMTPPD
                     GESRPVNIDPGFITLSKLVLASTKDHAHRLYLGQGIYGEITLAWKGHSFVSLPWSYPD
                     YVSLIPFLGEVRNRLQTMIRETGTDPALIR"
     gene            1587..1961
                     /locus_tag="PLD82_07810"
     CDS             1587..1961
                     /locus_tag="PLD82_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65323.1"
                     /translation="MNRRIMAALILAFALTGCAIFSSNKSDISSFDRDYLENKYVGKT
                     GWAKTDLGGIKTGEQLTVSAIDFDVRTFVRVKSGSTDHFFIAYNNKDMVPNEDQKVRV
                     SYDIATIEREINDRISFLKPGN"
     gene            complement(2001..2225)
                     /locus_tag="PLD82_07815"
     CDS             complement(2001..2225)
                     /locus_tag="PLD82_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65324.1"
                     /translation="MGRSSFLERTPLGGSEVERWASEYNLALFPEERRGSLLAEKLET
                     ACRMLRAEDPVSEISQATGLTPEEIDTLLY"
BASE COUNT          634 a          838 c          669 g          548 t
ORIGIN      
        1 gtcgctcaag ccgggctccg tagcgggcga gctgaccgat ccccagggcg gcctgaatat
       61 ccgtcatgtt atgtttatat cctgatactt caatatcgta ttcccagctt ccggccgcca
      121 tcttggccag ggcgtctttg gactggccat gcaaagccaa aagtcgaacc cgacgctcaa
      181 agccttccga taccgggcct gaggactgtt ccccaaaatg attaccaccc cctcgaccca
      241 aatccgcaaa ggccagggcg ccaccctccg cagtagtcag attcttgacg gcatgaaagg
      301 agtatgcagc catgtcggcc cactcgggga cacgcttccc ggaaaagctc gcgcccagtg
      361 cgtgggccgc atccgccaaa atggccgccc ccccgatgct ggcctgaaac tcattactcg
      421 ctacaaactc ccgtttgtgt gagtcgatgg cagtcctgag tgcccggtag ttacagggaa
      481 tcccggcaaa atccacggag atcacggcct tggtgcgcgc attgaccgcc gcttcgaccg
      541 cagccggatc gatggcaaac ccctcggcac caatatcgac cagcacaagc cgtgccccgg
      601 tatgcctcac gatattggca gtcgctgcat aggtgtagac cgtagtaacc acctcatccc
      661 caggaccgat tcccaaggcg aggagggcca tctccatggc agcagtcgcg gagttgaggc
      721 agatcacgtg cgacactccg agatacgcgc gcaatccgga ctcaaactcc ctcacggccg
      781 gcccggtcgt aatccaaccg gaacgcagcg tggccacaac acgtgcgatg tcagcctcat
      841 caatgtcggg ccggttaaac ggaacaagta tcttgtcggt gctcattcac gaaatctatt
      901 gcaaaacacg tcaacaatgg gcagtcttga aacgagaatc agaccggagg aattcatgtc
      961 acgctacgcc atcgatgccc gggaaacacc ggagccgctt tccctcccgc tggttgccat
     1021 catggctggc agtgatatcc tgatccaagc tgttcaggaa cgacttgttg aaacgtacgg
     1081 accagttttg gccgcaagtg aaccgtttct ttttgacaat ttcacaggat attacaaaaa
     1141 agaattcgga gaaggcatgc acaaacaggt ttgcatcttc cggacccttc gcgaacagcg
     1201 tctcctgccg gacgacaagc acgccagctg tgccatcgaa cgggatatga cgcccccgga
     1261 cggggaatcc cggcccgtca atatcgatcc cggctttatc acgctttcca aactggtcct
     1321 ggccagcacc aaggaccacg ctcatcgcct ctatcttgga cagggaatct atggagagat
     1381 caccctggcc tggaaagggc attcctttgt ctccctgccc tggtcctatc cggattacgt
     1441 ctccctgatc cccttccttg gtgaagtgcg caaccgcctt cagaccatga tccgggaaac
     1501 cggaaccgat ccggcattga ttcgttgata ctgcgcgaat gcggttgatg cagtatactt
     1561 tgagaacaat ctgcacggag gttctaatga acagacgcat catggcagca ctcatcctcg
     1621 cgtttgccct gaccgggtgc gcgatatttt cgtcaaacaa gagcgacatc tcgagctttg
     1681 accgcgacta tctcgaaaac aaatatgtcg gaaaaaccgg ctgggccaaa acagatctgg
     1741 gtggcatcaa gaccggcgag cagttgacgg tttcagccat tgattttgac gtccgcacct
     1801 ttgtccgcgt caagagtggc tcaacggacc atttctttat cgcctacaac aacaaggata
     1861 tggtacccaa cgaggaccag aaggtccgtg tctcgtacga catcgctaca atcgaacgcg
     1921 agatcaacga ccggatcagc tttctcaagc caggaaactg atccaccacc cgccctgcgg
     1981 ggcgggtacc gcaccagtca tcaatagaga agggtgtcga tttcttccgg tgtcaatccg
     2041 gtcgcttggc tgatctcgct gaccggatcc tcagccctga gcatccggca ggccgtctcc
     2101 agcttttccg ccagaagact gccccgcctc tcttccggga ataatgccag attgtactcg
     2161 ctcgcccagc gttccacctc ggaaccaccc aagggtgttc tctcgagaaa gctgctcctg
     2221 cccatgatgt tgccctgtcc tctcgcaccg aatggcgcac aaaaatgcca atcccggaac
     2281 caaggatagc aaaatgctag aaataattca agcggggtcg tgtcgcgaaa aacgagccgg
     2341 gcaggggcaa gcaaaaaacg tatctctctt ctgaaaatgg caagcaatga acggcttgct
     2401 gacctgtcaa tacccctgtc tggcccgctc atcataagat acgcaaccac tccccatgga
     2461 gcaatccccc acgacccgtg aattggcccg tccccctcgg tatgcaatcc gggacaagat
     2521 acaggcacgg ctgcacgctg cctgatgcgc cagaatcccg aaaaaatcga attgctccgc
     2581 gcccaccgga ttttcgtcca acctcgttgc tgatgcttca aacaatcgtg tctctccctg
     2641 tggttcttgc ccgggagcat ttcataccgc acacgccata cttcagagc
//
LOCUS       DAOSHE010000289         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36441_length_2689_cov_2.862946, whole genome shotgun sequence.
ACCESSION   DAOSHE010000289 DAOSHE010000000
VERSION     DAOSHE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36441_length_2689_cov_2.862946"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLD82_07820"
     CDS             complement(<1..1126)
                     /locus_tag="PLD82_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG65325.1"
                     /translation="MLKENLVQTLEEAIRNHWYAPALADYGSAACSYGQVAERILRLH
                     GLFDRFGVGKGDKIALIGKNSVNWCLVYLAAVTCGAVIVPILPDFSGEDVQHIVNHSD
                     AVLLFASEGVSEKLDEGSMKKLDAILSLTDFSLIYAKRSSWMGYPEKAARNFADKYGR
                     FLSPDNFRFPRVKNAETAAIVYTSGTTGFSKGVMLAHNSLIANIRFARTHMPLRAGDR
                     IVSFLPLAHAYGCAFEFLFPFCLGCDITLLGKTPSPKIILQAFSEIRPRLVLSVPLVI
                     EKIYRSKIRSKIEKTPVRLLRKVPGIGGMIEKKVLRALNSAFGGNFHEIIIGGAPFNA
                     EVAEFLEKIGFRFTVGYGMTECGPLISYTGSGKFRLHSVGQ"
     gene            complement(1157..1753)
                     /locus_tag="PLD82_07825"
     CDS             complement(1157..1753)
                     /locus_tag="PLD82_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65326.1"
                     /translation="MRDSDQRAQFVRGVMRRTGSAAAPEWAPGSLARLSGFLAVWLLA
                     LAATLVYRVFAIALSGKPLPATVTDVLVAAGIAWVLCFVFWRLGRIALASGFSFPRMV
                     MGGALFAPCLAFFVRAIQPGATGKGWLILGAIVLFSIGMLPTILRIVQGLTRRRRGYD
                     EGHAHWRSLPLMVFECILLIGGLFAGAAWFDVLIRSSS"
     gene            complement(1851..>2689)
                     /locus_tag="PLD82_07830"
     CDS             complement(1851..>2689)
                     /locus_tag="PLD82_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65327.1"
                     /translation="KSCLGRHCSEWQADICNTEKCGIRSLRSGKPVTNYMQEYPDRPS
                     TLMQVDTSYIHDEMGRVIGHIEFVTDIDATNRLRLTTERIAAATEEASASLEQMSAGM
                     QQTAANSAEIDKLMDDVRKVANASLVSMKDVSEAMSAVSASGTETSKIIRGIDDISFQ
                     TNLLALNAAVEAARAGSAGAGFAVVADEVRNLAQRAAGAAKSTSSLLEEMKERIDAGS
                     RHVGSASGTLGTLGEATSRGSELIKEIATAIQQQSQGITQITASVADLNDVAQNALAS
                     KN"
BASE COUNT          656 a          803 c          697 g          533 t
ORIGIN      
        1 actgcccgac cgagtggaga cggaatttac cgctcccggt atagctgatc agggggccgc
       61 attccgtcat cccgtacccg accgtaaaac ggaacccaat tttttcgaga aattcggcca
      121 cctcggcatt gaagggagcc ccaccaataa tgatctcgtg aaagttcccg ccaaaggcgg
      181 aattgagcgc acgcagaacc tttttctcga tcatcccccc tattccaggc accttgcgca
      241 gaagccggac cggggtcttc tcgattttgg atctgatctt cgagcggtaa atcttttcga
      301 tgacaagagg aacggagagg accagcctgg gccggatctc cgaaaatgcc tgaaggatga
      361 tcttgggcga aggggtcttg ccaagcaggg taatatcaca accaagacaa aacggaaaca
      421 gaaattcaaa agcacatcca taggcgtgtg ccagcgggag gaaggaaaca atccggtcac
      481 cggcacgaag cggcatatgg gtccgggcaa agcggatatt ggcgatcaga ctgttgtgcg
      541 ccagcatgac acccttggaa aaccccgtcg ttcccgaggt atagacgatc gctgcagtct
      601 cggcattctt gactcgcgga aaccggaaat tgtccggcga aagaaaccgc ccgtacttgt
      661 ccgcaaaatt cctggctgcc ttctcgggat accccatcca agaagagcgc ttggcataga
      721 tcagggaaaa atccgttagc gagaggatgg catccagttt tttcatgctc ccctcgtcca
      781 gcttttcgct gaccccttcg gaagcaaaga gcaggaccgc atcggagtgg ttgactatgt
      841 gctgcacgtc ctcgccggaa aaatccggaa ggatggggac aatcactgct ccgcaggtca
      901 ccgcagcgag gtagaccaga caccaattga ccgagttctt cccgatcagg gcgatcttgt
      961 ctcccttgcc gacgccaaag cggtcgaaga gtccgtgcag cctgaggatc cgctcggcaa
     1021 cctggccgta cgaacaggcc gcagagccat agtccgccag tgccggggca taccagtggt
     1081 tgcggatcgc ctcctcaagt gtctggacca ggttttcctt gagcatgagc gtgtactcct
     1141 tgcagtcttc tccccgtcaa gaggacgagc gtatcaacac atcaaaccag gctgcaccgg
     1201 caaagaggcc cccgatcagc agaatgcatt caaaaaccat caatggaagg gatcgccaat
     1261 gggcatgtcc ctcatcatat ccccgccgcc gtcgtgtcaa tccctgaaca atacggagaa
     1321 tcgtcggcag catgccaatc gaaaagagaa cgatcgcccc cagaatgagc caaccctttc
     1381 ccgtcgcacc cggctgaatc gcccgcacaa agaacgccag gcagggggca aagagtgcgc
     1441 cacccatgac catccgggga aaggagaaac cgctggcaag cgcaatccgt ccaagccgcc
     1501 aaaacacgaa gcacaggacc caagcgatcc ctgcagcgac gagaacatcc gtgactgttg
     1561 cgggaagcgg cttaccggaa agcgcgatgg caaacacacg atagaccagg gtcgccgcca
     1621 gagcaaggag ccagacggcc aaaaagccgg agagacgtgc caaagagcca ggtgcccatt
     1681 cgggagcagc cgcactcccc gtcctcctca ttacaccacg cacaaattgt gcacgctgat
     1741 cactgtcccg catgctcatc cccctgatac cgcagtgtac aacgacttga ccggaaaaat
     1801 caagcaagct tgcgtggcaa gacgaacaga aaaacctgtt tccatggcaa tcagtttttt
     1861 gacgccagag cgttctgtgc aacatcattc agatcagcga ccgaggctgt aatctgggtg
     1921 attccctgag actgctgttg aatggcagtg gcgatctcct tgatcagttc actccctcgg
     1981 gaagttgcct ccccgagagt ccccagtgtt ccggaagcac ttccgacatg tcgggaacct
     2041 gcatcgatcc gttccttcat ttcttcaaga aggcttgatg tactcttggc agcccctgct
     2101 gcacgttgtg ccagattcct gacttcatcg gctaccacgg caaacccggc tccggctgat
     2161 ccggcccggg cggcctcaac agccgcattc aaggcaagca gattggtctg aaaagagata
     2221 tcatcgatcc cccggataat tttcgaggtt tctgtcccgg atgcactgac tgcactcatg
     2281 gcctcactga catccttcat ggatacaagt gacgcgttcg caactttccg gacatcatcc
     2341 atcaacttgt cgatttctgc agaattggct gccgtctgct gcatcccggc cgacatctgt
     2401 tcgagtgatg cggatgcctc ctcggtcgca gccgcaatcc gctctgttgt caaccgcaga
     2461 cggttggtcg catcgatatc cgtgacaaac tcgatatgac cgatcacgcg acccatctcg
     2521 tcatgaatgt acgaggtatc aacctgcatc agggtggaag gacggtccgg gtattcctgc
     2581 atataattcg tcaccggctt tccacttcta agactccgta tcccgcattt ctcggtattg
     2641 cagatatcgg cctgccattc ggaacaatgc cgtccaaggc aggacttct
//
LOCUS       DAOSHE010000290         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36471_length_2687_cov_4.756459, whole genome shotgun sequence.
ACCESSION   DAOSHE010000290 DAOSHE010000000
VERSION     DAOSHE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36471_length_2687_cov_4.756459"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLD82_07835"
     CDS             complement(<1..592)
                     /locus_tag="PLD82_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65328.1"
                     /translation="MTRTLITILVFLATSPGLMALAPLSKTSVSIASDFEGRPTFSRA
                     NEFILLDNGRALFLGTSARFETRHTVYILKTDSMVRVPLLLSESLTGQLGKGHVHSLV
                     HYNSDDGRMAIIWERERQGRKELVAVSGRLVQGEISRSVVLAKQEGRELLAVRPTIGH
                     SGSGPFPLAISRQNEIGRYKYEDRGRVAISLIHEDGS"
     gene            778..1623
                     /locus_tag="PLD82_07840"
     CDS             778..1623
                     /locus_tag="PLD82_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65329.1"
                     /translation="MKALFAWLAKWTAKLDAEALGFDMATAMAMVLVQQIAGSDRQFL
                     GMLVGPVAHLVIIAAIVIILVRLKYLVVTVRETGSRGLAMVVSGALGLLSIVQPIFLT
                     GVAAKLGIVTPTAPDMLSFALGLSFIFGAVIGFDEEKKFDPLYSGLSTLGSILFFADL
                     PLQFGSSFLAVALVDVAVILAWVVLKFRGKPVRGLDSFPRETEPRHWGLRLLVSTGVG
                     LCLWAWWQAVLLPILKPEFDLLTRILLLYMTGPLLYRIALVIKPPVNPINAILGLAAI
                     VWALV"
     gene            complement(1898..>2687)
                     /locus_tag="PLD82_07845"
     CDS             complement(1898..>2687)
                     /locus_tag="PLD82_07845"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG65330.1"
                     /translation="IPRIYTNKPRTTGEGYKGMMHQPKANEAPDVVEGLKALRRLHVK
                     AIAQTGLPAADEMLYPENYPYVEDLLTYVAIGARSVENQLHRLTVSGLDIPVGMKNPT
                     SGDLGVMLNSIQAAQGRHQFIYNRWQVETNGNPWAHAILRGAVDHHGRAVPNFHYEDI
                     METIALYGQRGLKHPSILIDTNHANSNKQYKEQIRIAKEVMTNRRLSPEIRQTVKGLM
                     IESYLVEGAQSPTSTPHTRGQSVTDPCLGWEDSERLVLDLAEMA"
BASE COUNT          456 a          726 c          854 g          651 t
ORIGIN      
        1 cgctgccgtc ttcatggatc agggagatgg ctacccggcc ccggtcttcg tacttgtagc
       61 gtccgatttc attctgtctg gagatggcaa gagggaatgg accgctcccg gaatggccga
      121 tggtcggtcg gacggccagg agttcacggc cttcttgttt tgccagaacg acggagcgac
      181 tgatctcgcc ctggacgagg cgtccgctga ctgcaaccag ttcttttcgc ccttgccgct
      241 cgcgctccca gatgatggcc attctgccgt catcgctgtt gtagtgaacc agggaatgca
      301 catggccttt tccgagttgt ccggtgaggg attcactcag gagcagggga acgcgcacca
      361 tgctgtctgt cttgaggatg tagacggtat gtcgggtctc gaagcgtgcg cttgtaccga
      421 gaaacagcgc ccggccattg tccagaagga tgaactcgtt tgcccgtgaa aacgtcggcc
      481 ttccttcaaa atcgctggcg atggagacag aggtcttcga gagcggggct agcgccatca
      541 ggcccgggct tgttgccaga aataccagta tcgtgatcag ggtgcgggtc atgtcaatcc
      601 tccatagcgg gacgttccat tcgagtatag agcaaaaagg aatttgtctc aacccgggac
      661 gcccattttt ggtcaacgca cgcagcggtg gacgaagcgg tcgggctgga tgtatactgc
      721 ccggagtgga ttccgggatt ggaaaaacac gaagtgtggt tgtgtggagg agagcgtatg
      781 aaggccttgt ttgcctggtt ggcgaagtgg acagcaaaac tcgatgccga ggcgctcggg
      841 tttgacatgg cgacggccat ggccatggtt cttgtgcagc agatcgctgg gagcgaccgc
      901 cagttcctgg ggatgctggt cggaccggtt gcgcatctgg tgatcatcgc cgccatcgtg
      961 atcatccttg ttcgactcaa ataccttgtg gtgacggtgc gcgagaccgg ctcccgggga
     1021 ctggccatgg tcgtgagtgg tgcactcggg cttctctcca tcgtacagcc gatctttttg
     1081 accggggtgg ccgccaaact ggggatcgtg acgccgacgg cccccgacat gctctccttt
     1141 gcactcggtc tctcctttat cttcggggcc gtgatcggtt tcgacgagga gaagaaattc
     1201 gatccgctct actccggtct ttcgaccttg gggagtatcc tcttttttgc agatctgccc
     1261 ctccagttcg ggtcatcctt ccttgccgtc gctctggttg atgtggcggt gatcctcgcc
     1321 tgggttgtgc tcaaattccg cgggaagccg gtgcgtggtc tggacagttt tcccagggag
     1381 acggagccga ggcactgggg gttgcgtctc ttggtctcga caggcgtggg tctctgcctc
     1441 tgggcctggt ggcaggccgt tctgctcccg atcctcaagc cggaattcga tcttttgacg
     1501 cggatcctgt tgttgtacat gaccgggccg cttctctaca ggattgccct tgtgatcaaa
     1561 cccccggtca atcccatcaa tgccatcctc gggttggcgg ctatcgtctg ggcgctggtg
     1621 taggactgga cgagtctgta tttccgacga tgccggtttt ttgggtagac ggggatgcgc
     1681 aacatcgtgt ggcagagtca atcgcctgca ccgggcggtc cgccattcac caaaaaaaac
     1741 gcaacgggtc cggtcccgca tgggcataac cgtccgattg ccggtgctgt ccgctttctc
     1801 tggcgggatg cgcgagtgca tcccgcaaaa tgatacgttt gagtccgggg agtctccggc
     1861 tcatgacagt gcgaatgcag acagccagaa aaccgtgtta cgccatctcc gcaagatcca
     1921 gcacgagacg ttcgctgtct tcccagccga ggcaggggtc tgtgactgat tgcccacggg
     1981 tatgcggtgt gctggtggga ctctgggcgc cctcgacgag atagctttcg atcatcaggc
     2041 ccttgacggt ttgccggatc tccggagaga gtctgcggtt ggtcatcact tcttttgcaa
     2101 tgcggatctg ttctttgtac tgcttgttgg agttggcgtg gtttgtatcg atcaggatcg
     2161 acgggtgttt gagtccgcgc tggccgtaga gggcgattgt ctccatgatg tcttcgtaat
     2221 ggaaattcgg aacagcccgt ccgtgatggt cgacggcccc cctgaggatg gcatgggccc
     2281 aggggttccc gttggtttcg acctgccagc ggttatagat gaactggtgt cgaccctgtg
     2341 cggcctggat cgagttgagc atcactccga ggtcacccga ggtcgggttt ttcattccca
     2401 ccgggatatc gagaccgctg accgtcagcc ggtgaagctg gttttcaacc gaccgggcgc
     2461 cgatggccac gtaggtcagg aggtcctcga cgtaggggta gttttcgggg tagagcatct
     2521 cgtcggcagc gggcaagccg gtctgggcga tggccttgac gtggagacgg cgcagggcct
     2581 tgagtccctc gaccacatcc ggggcttcgt tggcctttgg ctggtgcatc atccctttat
     2641 agccttcacc cgttgtccgt ggtttgttgg tatagatccg cggtatc
//
LOCUS       DAOSHE010000291         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36870_length_2668_cov_4.080367, whole genome shotgun sequence.
ACCESSION   DAOSHE010000291 DAOSHE010000000
VERSION     DAOSHE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36870_length_2668_cov_4.080367"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLD82_07850"
     CDS             <1..1019
                     /locus_tag="PLD82_07850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65331.1"
                     /translation="TETLGIVARTVDISLALVLRAVILSILLAGLFVRNIRKLARAAA
                     RVGQGDLETTIDIRSRDELGRLGHEFNSMVSRIRESRAALVEKSRMEEELRIAETIQK
                     GLLPVRFPEIPGYAFAGYYSAQSEAGGDYYDVLESSLVGGGRVGLISADVSGHGVGAG
                     LVMTMARTLFHAQAADCPDPAQVLSRMNPQLFRDTPPAIFVTAFYAILDTGTHGITWA
                     SAGHNPALCARAGGRVDRCTSDGLPLGMADGPTFDSIIAQGSSSLAIGDVFLLYTDGV
                     TEAMNAEREEYGEERLADALKRASGLEPSVILDAIVADLAVFTGDLPQEDDISMLVVK
                     RQVR"
     gene            1016..2665
                     /locus_tag="PLD82_07855"
     CDS             1016..2665
                     /locus_tag="PLD82_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOG65332.1"
                     /translation="MIPSAADLLKDLTAPRTGNTIEVVQAKNGQPVARRNGRYVDSSH
                     DPEAAARRRLTGLALEGRRLAVVFGDGFGYLARQLVAAGLDVLLIEADPDLPLAGYRA
                     LGHAAWAGFALSFCADSQEAGFWEARIRPDLQAPDVLVVPPAAGADPCLVQAAKTLVE
                     SLRRRMIDFETTAWFGPQWVINILANLAVLQADSGRVLSPASLPMGGDWVIAVPGPGL
                     DEVLPELAGMRKGCTLLALAPAVKALRAAGLEPDLVCSSDGGWANRLHLAGENLPGVP
                     LVFPLMLAAGIAHAWAGPLVPVSTGTALERQVVSGLPPMGETPSVALFALGIAIACRA
                     GRIVLAGQDFASYSCKTHARGYRFDEDLLHSACRAAPVEKHLDMTWRRRDRMVSGWRA
                     DPKMFLYRERLLELGRTMRLPLFRLGSSPFLDAIPAFPGDWRAGLVGRSVRAEWQIQD
                     TVLRDWLARLAGVVGTEVWGTVSGPGDGKDRHMEEEEALLALIRDGSLKPLFELGQAP
                     ILRQVLRGRPVDGLPDPRPWLRRVVRRLGILLNNPAGDADR"
BASE COUNT          424 a          793 c          911 g          540 t
ORIGIN      
        1 gcaccgagac cctggggatt gtcgcccgga ccgtcgacat ctcgctggcg ctggtcctgc
       61 gggcggtgat tctctcgatc ctcctggccg gactctttgt ccgcaacata cggaaactcg
      121 cccgggccgc cgcccgggtc gggcagggtg atcttgaaac gacaatagac atccgcagcc
      181 gggacgaact ggggcggctc ggacatgagt tcaacagtat ggtgagccgc atccgtgaat
      241 cacgggcggc cttggtcgag aagagccgga tggaagagga gttgcggatc gcggagacca
      301 tccagaaagg tctcctcccc gtccgttttc cggagatccc cgggtatgcg tttgcgggat
      361 attacagcgc ccagagcgag gcgggtggcg attattatga tgtccttgaa tcatcgctgg
      421 ttggtggcgg acgggtgggg ctgatcagtg ccgatgtttc cggacacggg gtcggggccg
      481 gactggtgat gaccatggcc cgcaccctct tccatgctca ggctgcggat tgtccggatc
      541 cggcccaggt cctctcccgg atgaatcccc aactcttcag ggatacaccg ccggccattt
      601 ttgtgaccgc gttctacgcc attcttgata ccgggacgca cgggatcacc tgggcctcgg
      661 cggggcataa tccggcactc tgtgcccggg ccggcgggag ggtggaccgt tgtacctcgg
      721 acggattgcc actcggcatg gctgatggcc cgacttttga cagcatcatc gcccaaggga
      781 gttccagtct cgccatcggg gatgtcttcc tgctttacac ggacggcgtg accgaggcga
      841 tgaatgccga gcgggaggag tatggagagg aacgcctggc cgacgcgctc aagcgggcct
      901 cggggttgga accttccgtt attcttgatg ccatcgttgc ggacctggca gtctttaccg
      961 gcgacctccc ccaggaggat gacatctcca tgctggtcgt caaaaggcag gtccggtgat
     1021 tccgtctgct gccgatcttc tcaaagacct gacagcaccc cgaacgggga ataccatcga
     1081 ggtggtgcag gccaaaaacg gccaaccggt cgcgcggcgt aacggccgct atgtggacag
     1141 cagccatgat cctgaggccg cggcgcgcag gcgtctgaca gggcttgcgc tggaaggcag
     1201 aaggctggct gtcgtgtttg gcgatggatt cggctatctt gcccgtcaat tggtcgccgc
     1261 cgggctggac gttctcctga tcgaggcgga tccggatctg cccctggccg gctaccgggc
     1321 cctgggacat gccgcctggg ccgggtttgc gctgtcattc tgtgcggact ctcaggaagc
     1381 cggattctgg gaggcccgga tccggccgga tctgcaggca cccgatgttc ttgttgtacc
     1441 acccgcagcc ggtgcggatc cgtgtcttgt ccaggcggcg aagaccctgg ttgaatcact
     1501 ccggagacgg atgatcgatt ttgagacgac ggcctggttc gggccgcaat gggtcatcaa
     1561 tatccttgcc aacctcgcgg tgctgcaggc cgattccgga cgggtgctct cgccggcatc
     1621 cttgcccatg ggcggggatt gggtgatcgc tgtccccggg ccaggcttgg acgaggttct
     1681 ccctgaactc gcgggcatgc ggaaggggtg cacgcttctg gccctggccc ctgcggtcaa
     1741 ggcgctccgg gccgccggac ttgagccgga ccttgtctgc agcagtgacg gtggctgggc
     1801 caacaggctc catcttgccg gtgagaatct tccaggagtc ccccttgtgt ttcccctgat
     1861 gctggctgcg gggattgcgc atgcctgggc tggccccctg gtcccggtct ccacagggac
     1921 ggcgctggag aggcaagtgg tgagcgggct tcccccgatg ggcgagacgc cgagtgtggc
     1981 cctctttgcg ctggggattg cgatcgcatg ccgggccgga aggattgttc ttgccgggca
     2041 ggactttgcc tcgtattcct gtaaaaccca tgcccgtggc taccggtttg acgaggatct
     2101 gctccattca gcatgccggg ccgcccctgt cgaaaaacat ctggatatga cctggcgccg
     2161 tcgtgaccgg atggtgtccg gatggcgggc cgatccgaag atgtttctct accgggagcg
     2221 gcttcttgaa ctggggcgga cgatgcgtct gccgctcttc cgcctcgggt ccagtccctt
     2281 cctcgatgcg ataccggcgt ttcccgggga ctggagagcc ggccttgttg gacgcagcgt
     2341 ccgggccgaa tggcagattc aggatacggt gctccgggac tggttggcga ggctcgccgg
     2401 ggtggtcgga acagaggttt ggggaacagt ctccggtccc ggggacggga aggataggca
     2461 catggaggaa gaggaagccc tcttggcgct gatccgggat ggctcgctca agcccctgtt
     2521 tgaactgggc caggccccca tcctgcgcca ggtattgcgc gggagaccgg ttgacggctt
     2581 acctgatccc cgaccatggc tgcgccgggt cgttcggcga ctgggtattc tcctcaataa
     2641 cccggccggg gatgccgata ggtagggc
//
LOCUS       DAOSHE010000292         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_36894_length_2667_cov_3.565084, whole genome shotgun sequence.
ACCESSION   DAOSHE010000292 DAOSHE010000000
VERSION     DAOSHE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36894_length_2667_cov_3.565084"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLD82_07860"
     CDS             complement(<1..637)
                     /locus_tag="PLD82_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG65333.1"
                     /translation="MYVKFWGVRGSIPTPGPKTVRYGGNTSCIEIRLESGQLLIIDAG
                     SGIRELGNWLLANDQKKGPLKTDIFLTHTHWDHIHGYPFFTPTYIKGNEFRIHGPVNY
                     SGKLEKIFSAQMDYTYYPVKLEDAGAKVSFNELKEGTIYVGGAKVTTCYLNHPILCLG
                     YRIEADDRVVCTVYDHEPYVNIFSTDGRLDTDEAREAQESVDMANAKIRKHI"
     gene            complement(652..1518)
                     /locus_tag="PLD82_07865"
     CDS             complement(652..1518)
                     /locus_tag="PLD82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG65334.1"
                     /translation="MSLEKNIEKIIDSMPSLPITVAKIIEITKNPQSTPSDLNKVITL
                     DPVLTAKVLKLVNSAYYSLSNQITSIVKAIILLGINTIKNLSISTAVLGTMRKKTGTT
                     ALNMDGFWRHCLAVGVTARDIAARIGVDPKLRDEYFIAGMLHDIGKIVINEHFSDRYL
                     QTIHGADSARVSLWKKELELFETDHARAGRLVAEKWQLSPPLAEAIGLHHSPLEASPE
                     NKRLVAAIAAADAFCIENEVGFSGNRIPDSIPDEVWQTLGLKEDDLFGIEDTINEELE
                     RASVFLRISEKA"
     gene            complement(1757..2383)
                     /locus_tag="PLD82_07870"
     CDS             complement(1757..2383)
                     /locus_tag="PLD82_07870"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG65335.1"
                     /translation="MPDRNSIIQSASADRLAKLIRARMEPGADKDAIDRRIWDLFGET
                     WCIMFTDLSGFSRNVARFGIIHFLQTIQESERLLVPVISEHDGILLKTEGDSMLVIFR
                     TAQRGLECALAMQHLLEPYNAAKAPEEQVLLCIGLGSGPVLKIGDSDVFGTEVNAASK
                     LGEDTARAREILVTGSVEEACRNTPGCSFEPIDEIPPGAERAFRVLYS"
BASE COUNT          529 a          676 c          846 g          616 t
ORIGIN      
        1 cgatgtgttt ccggatcttg gcattggcca tatcgacgga ttcctgggcc tcccgggcct
       61 cgtccgtatc gagccggccg tctgtgctga agatgttgac atagggctca tggtcgtaga
      121 cggtgcagac gacgcggtca tccgcttcga tccggtagcc gagacagagg atcgggtggt
      181 tgaggtagca ggttgtgacc ttggcgccgc cgacgtagat ggtgccttcc ttgagttcgt
      241 tgaaactgac cttggcgccc gcatcctcga gtttgaccgg gtagtaggtg tagtccatct
      301 gggccgagaa tattttttcg agtttcccgg agtagttcac gggaccgtgg atgcggaatt
      361 cgtttccctt gatataggtc ggggtgaaaa agggatagcc gtggatatga tcccagtggg
      421 tatgggtcaa aaagatgtcg gttttgaggg gacccttttt ttggtcattg gcaaggagcc
      481 aattcccaag ctcccggatt ccggaaccgg catcgatgat caaaagctgc ccgctctcga
      541 ggcggatctc gatgcaggat gtgttgccac catagcggac cgtcttggga cccggtgtcg
      601 ggatggatcc acgtactccc cagaatttga cgtacatgcg tatcctcccg gtcaggcttt
      661 ttcagagatg cggagaaaga ccgaggcccg ctcgagctcc tcgttgatgg tgtcttcaat
      721 accgaacaaa tcgtcttcct tgagcccgag ggtctgccag acttcgtcag gaatgctgtc
      781 cgggatccgg ttccccgaaa agccgacctc gttctcgatg caaaaagcat cagcggcagc
      841 gatggcagct acgagccgtt tgttttcggg agaggcctcg agcggagagt ggtgcagccc
      901 gatagcctcg gccaagggcg gggagagttg ccatttttcg gcgacaagac ggcccgcccg
      961 ggcgtgatcc gtctcgaaga gctccagttc ctttttccag agggagaccc gggcactgtc
     1021 ggccccatgg atcgtctgga gatagcggtc ggaaaaatgc tcgttgatga cgatcttgcc
     1081 gatgtcatgc agcatgccgg cgataaagta ttcgtcgcgg agcttgggat cgacaccgat
     1141 ccgggcagcg atatcccggg cggtgacgcc gacggccaga caatgccgcc agaagccatc
     1201 catattaaga gccgtcgttc cggttttttt gcgcatcgtt cccagaacgg ccgtgctgat
     1261 cgaaagattt ttgatcgtat tgatcccaag gaggatgatt gccttgacga tgctcgtgat
     1321 ctggttggag agactgtaat aggccgagtt gacgagtttg aggactttgg cagtgagaac
     1381 cggatcgagg gtgatcacct tgttgagatc gctgggggtg gattgcggat ttttggtgat
     1441 ctcgatgatc ttggccaccg tgatcggcag gctgggcatc gaatcgatga ttttttcgat
     1501 atttttttcg agactcacga cccgctcccc tggtatgtcc tgcgacttga attgtacaca
     1561 gtccgggatg gaaaaatcaa gagagccaat gcgtccgggg aacgtgagtt ccgggaagat
     1621 ccggccgttt ttccggtttt tcagggtacg gcgtccggaa cccttttcgc cgggcgggcc
     1681 tggttcggcg ggagcacgag cgtctccggc gacggagatg ctagtcaggg ggagaaagga
     1741 tcccgggagg gcgtgttcat gagtagagaa cgcggaaagc ccgttcggcg cccgggggaa
     1801 tttcgtcgat cggctcgaag gagcaccccg gggtgttccg gcaggcttcc tcgacggaac
     1861 cggtgacgag gatctcgcgg gcacgggcgg tatcctcgcc gagtttactg gcggcattca
     1921 cctcggtccc gaagacatca ctgtccccga tcttgaggac gggcccagac ccaaggccga
     1981 tgcagaggag gacctgctcc tcgggtgcct ttgcggcgtt atagggttcg agaaggtgct
     2041 gcatggcgag tgcgcattcc agtccgcgct gtgcggtgcg gaagatgaca agcatgctgt
     2101 ccccttcggt tttgaggagg atcccgtcat gctcggagat gaccggaacg aggaggcgtt
     2161 cggactcctg gatcgtctgt aaaaaatgga tgatgccgaa acgggcgacg ttgcgggaga
     2221 aaccggagag gtcggtaaac atgatgcacc aggtttcccc gaagaggtcc cagatccgcc
     2281 ggtcaatcgc atccttgtcc gcaccgggct ccatgcgcgc cctgatcagc ttggccagac
     2341 ggtcagccga ggcactttga atgatggaat tgcggtcagg catgggtaac ctccctggag
     2401 taaccggagt acgataccgt gttgcccccc gtgtgtcaag ggaggcgttg cttaaatttt
     2461 aagcaatttt gcagtccggg attaccgagt gatgtccgtt tttgccttca attcttgtaa
     2521 aagtgcaaaa aataaggggt tttctgaaat ttggccttgt gcggcggata cgttcgttgc
     2581 ttaaatttta agcaactgcg tactctccct gcgggatctt cgccggaatt atcgcgcgga
     2641 atccaccagt gcttgcaggc tgaccag
//
LOCUS       DAOSHE010000293         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37015_length_2661_cov_3.864160, whole genome shotgun sequence.
ACCESSION   DAOSHE010000293 DAOSHE010000000
VERSION     DAOSHE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37015_length_2661_cov_3.864160"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLD82_07875"
     CDS             <1..816
                     /locus_tag="PLD82_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65336.1"
                     /translation="ILLALTSLCAIYATGKELFSPEFGIGAVIIMALLPSFAAHASGL
                     FAITFGYAYTGIALWLLARSRRHDGLPSLILAGSCIALALHAEPLFLYLPVFLVLFWI
                     EAGAGMRQILPVVGGLVAGEVLLMLLSLAITGLPPFAGLAALFTPDKTLDPAGYTGLA
                     DLFGQLFTNPQVLPFTLLAVFGIGYTLRASSSSFPWQPMLLLAAAYFTLEFLPKSFSP
                     WTTLPKDGRMLAVLAPPLALVIGALLAGLGNRLVLRWLAIGATALAIPLLFLY"
     gene            836..1651
                     /locus_tag="PLD82_07880"
     CDS             836..1651
                     /locus_tag="PLD82_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65337.1"
                     /translation="MPSFDHLAKKETIVPGLIVLVLFSLVLANLVPSAPLAPGTVLYE
                     KNNFGYQPVMTTKASNVVRIHKQAGNFIQVQIDTPPARLWVPAQALPGRHSLPAQLFL
                     RTQAGQLGPTLPPFVEKHLTIRYGNDEKPGQPSSQHSSVHVLFSLFLLAVLMLSMKSQ
                     AVKNSLAVLIGCFVLFYSVYASGLVKSSLESPQAGIVRILDLVRTGPGMQIYCQSEDL
                     RTIVHSRHGNNITASPVSAMPAASRGYLLTRSPAGAGWKLIATDGTVQLYYRD"
     gene            complement(1639..2079)
                     /locus_tag="PLD82_07885"
     CDS             complement(1639..2079)
                     /locus_tag="PLD82_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65338.1"
                     /translation="MKVWISGIVHGSRLDGGQHGQEYRERIRRALAARYHDVVLSSPQ
                     ELNSGLPGDEETVFFRQLGDVISADVLVAYLPEASMGASIEMWEAWKNSRPILTISPL
                     SSVWTVRFLSSIVVPDLETFEARTADGCLDRFLLDRYERPRQSR"
     gene            complement(2122..>2661)
                     /locus_tag="PLD82_07890"
     CDS             complement(2122..>2661)
                     /locus_tag="PLD82_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65339.1"
                     /translation="EDGGRHLYGEELMALGTLEAQRGDWKNALRLMRAAVTRFPSSPG
                     LYDKGTVQTLHWQRQEFFLREAFRLAQVLDRRDPGDAQVMQELSLLARETGAGPEMVG
                     RYDKRLLALPWASRGDRESAFLRLAAGSLDQPLRLWSLVSRFGAVLSPAGRKKIAAEL
                     DTLARHDFWLRPMSGRTNW"
BASE COUNT          489 a          944 c          687 g          541 t
ORIGIN      
        1 atccttcttg cgctgacaag tctctgtgcg atctacgcga ccggcaagga actcttctcc
       61 cccgaattcg gtatcggagc cgtcatcatc atggcgctcc tgccctcctt tgccgcacac
      121 gcctcgggac tcttcgccat caccttcggg tacgcctata ccggcatcgc cctctggctt
      181 ttggcccgct cacgccgaca cgatggcctg ccctcactga tcctggccgg atcctgcatc
      241 gccctggcat tgcacgccga gcccctcttc ctctatctcc cggtctttct ggtccttttc
      301 tggattgaag cgggtgccgg gatgcggcag atcctccctg tcgtcggcgg acttgtcgcc
      361 ggagaggtcc tgctcatgct cctctcgctg gccatcaccg gcctgccccc ctttgccggt
      421 ctggccgccc tcttcacacc ggacaaaacc ctggacccgg ccggctacac ggggctggcg
      481 gatctctttg gccagctctt caccaatccc caggttctcc ccttcaccct gctggcagtc
      541 ttcgggatcg gctacaccct cagggccagc agttccagct ttccctggca accgatgctc
      601 ctgctggctg ccgcctattt cacactcgaa tttcttccga aatccttctc gccctggaca
      661 acccttccca aggacggacg gatgctggct gttctcgcac caccgctggc cctcgtgatc
      721 ggagcgcttt tggcgggact gggaaaccgg ctcgtcctgc gctggctcgc aatcggagcc
      781 acagccctcg ccattcccct tctcttcctc tactgaatcc acgaaaggaa cagccatgcc
      841 atcctttgat catctggcca aaaaagagac gattgttccg gggctgatcg ttctcgtcct
      901 tttttccctt gtcctcgcca acctggtacc atcagccccc ctcgccccgg gaaccgttct
      961 ctacgaaaaa aacaattttg gctaccagcc cgtcatgacc accaaggcct caaacgtggt
     1021 gaggatccac aaacaggccg ggaatttcat ccaggtccag atcgacacgc ccccggccag
     1081 actctgggtc cccgcccagg cccttcccgg ccgacattcc ctgccggccc agctcttcct
     1141 ccggacgcag gccggacaac tcggaccgac actcccgccc ttcgtggaaa agcacctcac
     1201 catccgttat ggaaacgatg aaaaaccggg tcagccatcc agtcaacact ccagtgtcca
     1261 cgtcctcttc tcgctcttcc tgctggccgt cctgatgctc tcgatgaaat cccaggctgt
     1321 caaaaactcg ctggctgtcc tgatcgggtg ttttgtgctc ttttactcgg tctatgcctc
     1381 gggcctggtc aaatcgtccc tcgaatcccc ccaggccggt atcgtccgga tcctcgatct
     1441 ggtccggacc ggaccgggga tgcagatcta ctgccaatcc gaagacctgc gcaccatcgt
     1501 gcacagccgt cacggaaaca acatcaccgc cagtccggtc tcggccatgc cggccgcatc
     1561 ccggggctat ctgttgaccc gcagcccggc tggagcagga tggaagctga tcgcgaccga
     1621 tggcacggtc cagctctact accgcgactg acggggacgt tcgtagcgat ccagcaaaaa
     1681 ccggtcaaga cacccgtcag cggtccgcgc ttcgaaggtc tcgagatccg gaaccacgat
     1741 cgaggagaga aatcggactg tccaaacact ggagagcggc gagatcgtaa ggatcggccg
     1801 gctgtttttc caggcctccc acatctcgat ggatgccccc atgctggcct cggggagata
     1861 ggccacgaga acatcggcgc tgatcacatc gccgagctgt ctgaaaaaga ccgtctcctc
     1921 gtccccggga agtccgctgt tgagttcctg cggagaggag agcacaacat catgataccg
     1981 tgcggccaag gcgcgccgga tcctctctcg gtactcctga ccatgttgtc cgccgtcaag
     2041 ccgtgagcca tgaactatgc cgcttatcca aaccttcatc cagaccctcc tttcagccag
     2101 tatactccga cgacacccgg cttaccagtt cgtccttccc gacattggtc gcagccagaa
     2161 gtcatggcgc gcgagggtat ccagctccgc ggcgattttt ttgcggccag ccggtgaaag
     2221 caccgcacca aaacgggata caagagacca gagccggaga ggctgatcca gggatcctgc
     2281 agcgagtctg aggaaggcac tctcccggtc cccccgcgag gcccaaggca gggcgagcaa
     2341 acgcttgtca taccgcccga ccatctccgg accggctccg gtctctcgtg ccagcaggga
     2401 gagttcctgc ataacctggg cgtctcccgg atctctccgg tccagaacct gcgccaggcg
     2461 aaaggcttcc ctgaggaaga actcctgccg ttgccagtgc agggtctgga ccgttccctt
     2521 gtcgtacaac cccggactgg acggaaaccg tgtcacggcg gcacgcatca accggagggc
     2581 gtttttccag tccccacgtt gcgcctcaag ggtgcccagg gccatcagct cctcgccata
     2641 gagatgccga cctccgtcct c
//
LOCUS       DAOSHE010000294         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37102_length_2657_cov_3.657571, whole genome shotgun sequence.
ACCESSION   DAOSHE010000294 DAOSHE010000000
VERSION     DAOSHE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37102_length_2657_cov_3.657571"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PLD82_07895"
     CDS             <1..1593
                     /locus_tag="PLD82_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG65340.1"
                     /translation="YGMVLREMKAVANRVGEEALFDRERFLQLPEIEQLQREFGDGTP
                     LAGPLAIRPEDRDSVAIVEEVWSRLVVATLLQKMLDENPRLSLADFCRQTGLEDNPGV
                     REFFFGMKYAGILMRDSRLVGSALATTDARRLGIEDSKLFEEYRLLIGNERPDSGERP
                     YFFYPAPIVESLKGPLYGTWLHGKICLYGLTASGTHDLNPVPMASRYPMVGVHATVAD
                     NILSSNPMTRVPRSVNLAIVLGLAILSGLLFLRTRSAVMAVSAFLMVCALVAVNWFFF
                     STQDLWLEIVPPLVLVVGFFVSAIVHNYLGEEREKKRIRGAFSRYVAPALVDQIILDP
                     GMLRLGGEKRVCTVFFSDIAGFTTLSEGLSPEQLVEFLNTYLTEVTDIILAHGGMLDK
                     YEGDAVMAVFGAPLLQPDHAERACRAALAVQARLSELRAAWLERGLPAFITRIGLNSG
                     EMVVGNMGSRERFDYTVIGDAVNLGSRLEGANKQYGTYLMISQATRDLAGPERFVTRE
                     LDMIRVKGKAEPVRVFELIDTV"
     gene            2058..>2657
                     /locus_tag="PLD82_07900"
     CDS             2058..>2657
                     /locus_tag="PLD82_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65341.1"
                     /translation="MKNTPSRRKILALIGIVAALSLFAFSLVQGYWYYNDFVTETDNW
                     TLGYRYARNGFFDNGNNASTISRAGGTYLRFTGVVNTNDANMDRWTGRAAYYIGAEYS
                     ASRTAPFGVEFLRGTTQLDPHGSTSNSESDRHSVAQTFWLFNGTPPTTDGGSFANSVF
                     MYEMMRMSANVSGNPVQPRSTWGRYVASEQRFNLKNALRP"
BASE COUNT          507 a          729 c          793 g          628 t
ORIGIN      
        1 tacgggatgg tcctccgtga aatgaaggcc gtggccaaca gggtcggtga ggaagcgctt
       61 tttgaccggg aacgcttcct gcagcttccc gagatcgaac agctccagcg cgagttcggt
      121 gatggaacgc ctctggcggg accgctggcc atccgtccgg aggatcggga ttcggtcgcc
      181 atcgtcgagg aggtctggtc acggcttgtc gtggcaaccc tcctgcaaaa gatgctcgac
      241 gaaaacccca ggctgagtct tgcggacttc tgccgacaga ccggccttga ggacaatcca
      301 ggagtgcgcg agttcttctt cgggatgaag tatgccggga tcctgatgcg ggattccagg
      361 ttggtaggga gtgcgttggc cacgacggac gccaggcggc tcggtatcga ggacagcaag
      421 ctctttgagg agtaccgcct cctgatcggg aatgaacggc ccgattccgg cgagcgcccg
      481 tatttctttt atccggcacc cattgtggaa agtttaaaag ggccgctcta cgggacatgg
      541 ttgcacggga agatctgtct gtacgggctg accgcctccg ggacgcatga cctcaatcct
      601 gtcccgatgg cttcgcgcta ccccatggtg ggtgtgcacg ccacggttgc cgataacatc
      661 ctttcatcca acccgatgac gagggtgcct cgctccgtca acttggcgat tgttctcggg
      721 ttggcgattc tttccggtct gctctttctc cggacacgaa gtgccgtcat ggccgtgtcc
      781 gccttcctca tggtctgtgc cctcgtcgcc gtcaattggt tctttttctc gacgcaggat
      841 ctctggttgg agatcgttcc tcctcttgtc cttgttgtgg ggttctttgt ctcggccatc
      901 gtgcacaatt acctcgggga agaacgggag aaaaaacgca tcaggggggc tttctcacgc
      961 tacgttgccc cggccctggt cgaccagatc attcttgacc ccggaatgct ccgtcttggc
     1021 ggtgaaaagc gggtctgcac cgtgttcttt tccgatattg ccggatttac gaccctctcc
     1081 gagggcctct ctccggaaca gcttgtcgag tttttgaaca cctacctgac cgaggtgacc
     1141 gacatcatcc tggcgcacgg ggggatgctc gacaagtacg agggagacgc cgtgatggcg
     1201 gtctttggcg cccccctcct gcaacccgac cacgccgagc gcgcctgccg ggcggccctt
     1261 gctgtccagg ccaggctctc cgagttgcga gctgcgtggc tggagcgcgg tctgcccgcc
     1321 ttcatcacga ggatcgggct caattcagga gagatggttg tcgggaatat gggttcgcgc
     1381 gagcgttttg actataccgt catcggggat gcggtcaatc tcgggtcgcg gcttgagggt
     1441 gccaacaagc agtacgggac ctacctcatg atctcccagg cgacccgtga tctggccgga
     1501 ccggagcgtt ttgtcacccg tgaactggac atgatccgtg tcaagggcaa ggccgagccg
     1561 gtccgggtct tcgaattgat cgacaccgtc tgagtttcct ccggggcccg tgacggtgcg
     1621 tctcctctcc cgggtttttt tgatgaacag cctcaggcca gctgcgggat ttcggttcgc
     1681 gcgacggtgc ggatccgggt cccgcgagga atcttgtaaa atagtgatga catcatgaag
     1741 gaacgggata caccctgccg atactcggaa gggtgtgtcc cgttgtatcc tcgcgggacg
     1801 aggtggtggg taaaatttac tcacatgtgt ttcaaattta ccacatgccg actcggactt
     1861 ccctggacga gggcgtctct ggtctccggt agcgtgcttt cgtgactgtc ggcggtgtga
     1921 attttattaa ggaaggttcc cgattgcatt ttgggcccga gtcattcagg attgcctgtg
     1981 tttccgggaa tatttcatcc aaacggtatg aaatttgcag tacaaaagaa tggtaaaatc
     2041 tgcacacgga gatagctatg aagaacacac cttcccgcag gaagattctc gcgctgattg
     2101 gcatcgttgc cgcgctcagc ctgttcgcgt tctcgcttgt gcaggggtat tggtattaca
     2161 acgattttgt tacagagacc gacaactgga cgctgggata cagatatgcc cgcaacgggt
     2221 ttttcgacaa cgggaacaac gccagcacga tttcgagggc tggaggaacg tatttacgat
     2281 ttaccggtgt tgtcaacacc aacgatgcaa atatggacag atggactggc cgggctgcgt
     2341 actacattgg tgccgagtac tcggcttccc ggactgcccc gttcggggtg gaatttttac
     2401 gcggtacgac acaactcgat ccacatggga gtacgtccaa ttcggaaagt gatcgacatt
     2461 cggtcgccca gaccttttgg ttgttcaatg gcacaccacc gactacggat ggtggatcgt
     2521 ttgccaattc cgtcttcatg tatgaaatga tgaggatgtc ggccaacgtt tcagggaacc
     2581 ccgtccagcc ccgcagtact tgggggcggt atgtcgcatc cgaacaacgc tttaatctga
     2641 aaaacgcgct tcggccg
//
LOCUS       DAOSHE010000295         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37125_length_2656_cov_3.405998, whole genome shotgun sequence.
ACCESSION   DAOSHE010000295 DAOSHE010000000
VERSION     DAOSHE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37125_length_2656_cov_3.405998"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..773)
                     /gene="fabG"
                     /locus_tag="PLD82_07905"
     CDS             complement(27..773)
                     /gene="fabG"
                     /locus_tag="PLD82_07905"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOG65342.1"
                     /translation="MLLKDEVAIITGAARGIGRSIAEKFAAQGSHLAIIDVNLEAAEQ
                     TAQELAKQYGVKTMAARTDVSNAEDVDAMVKKVLDTFGRIDILVNNAGVTRDNLMLRM
                     KDEEWDLVININLKGVFVCTRSVVRHMAKARKGNIINIASVVGQMGNAGQANYTASKG
                     GVIALTKTTAREFAGRNVRVNAVAPGFINTAMTEVLSEQVKTEMLRQVPLDRMGEPED
                     IANGCLFLASGMSAYITGHVLAINGGMLMQ"
     gene            complement(780..1703)
                     /gene="fabD"
                     /locus_tag="PLD82_07910"
     CDS             complement(780..1703)
                     /gene="fabD"
                     /locus_tag="PLD82_07910"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG65343.1"
                     /translation="MVAFLFPGQGAQSVGMCKDIHDTYPEAREAFETASRVLGYSMED
                     LCFSGPEEKLKDTRYTQPALLAASIAVLRVLEANEIMPDACAGHSLGEYSALVAAGMI
                     PYEDALRLVARRGELMAESDPEGRGGMAAVMGLSPEQISEVIKAAAKGRSLEAANFNC
                     PGQIVISGDKDAVADAESFANDAGATNFVTLQVSGPFHSSLMKPAAESFAADLAAASF
                     VEGHARVVSNVTASWHEPKQTVDLLKKQIYSSVRWEDSIRFLVANGVDAFIEVGPGKV
                     LRGLMRRIDKSLACQGSGDLESLEKILSQGR"
     gene            complement(1728..>2656)
                     /locus_tag="PLD82_07915"
     CDS             complement(1728..>2656)
                     /locus_tag="PLD82_07915"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOG65344.1"
                     /translation="NEDLSKRVDTSDEWIRTRTGIEERRIAPPGMNNSDLAIEASRKA
                     LDAAGITAEDLDMIIVATITPDMVFPATACLVQHALGAKHAATFDLEAACSGFIYGLS
                     VARQYIATGEYKNVLVVASEVLSRITDWTDRSTCVLFGDGAGAAILSAAEEGESDFLS
                     FFLGGDGKYSEILNMPAGGTRKPASHETVDAQEHSMKMNGPEVFKAAVMKMNHSMRAV
                     LKKAGYESQAIDLVIPHQANLRIIESLRKYLDLSEDKVYVNLQKYGNTSAASIAIALA
                     EVIESGRLKRGQLVGLCAFGGGLTWAACILRY"
BASE COUNT          607 a          728 c          687 g          634 t
ORIGIN      
        1 tcacgtagta tactaagctt ccatctttat tgcatgagca tgccaccatt gattgccagc
       61 acatggccgg taatgtaggc ggacatccct gatgccagga agagacagcc atttgcaata
      121 tcttccggtt cacccattct gtcaagtgga acctgacgaa gcatctctgt ttttacctgc
      181 tcggagagaa cctccgtcat tgcagtattg atgaaaccgg gagcaacagc attgactcgg
      241 acgtttcgac cggcgaactc cctggccgtt gtctttgtca atgcgatcac accacccttg
      301 gatgccgtgt aattggcttg accggcattc cccatctgcc ccacaacgga ggcgatgttg
      361 atgatattcc ccttgcgtgc tttggccatg tgccggacga cgctccgggt acagacaaaa
      421 acacccttga gattgatatt aatgacaaga tcccattctt catccttcat gcgaagcatg
      481 agattgtcac gggtaacgcc cgcattgttt acgagtatat cgatccggcc aaaggtgtca
      541 agaactttct tgaccatggc atcgacgtcc tcggcatttg aaacatctgt ccgtgcagcc
      601 atcgtcttga ctccgtactg ctttgccaac tcttgagcgg tctgctcggc tgcttcaaga
      661 ttgacatcga tgattgccag atgcgaccct tgggcggcga atttttcggc aatggatcgg
      721 ccaattcccc gggctgcacc cgtgatgatg gccacttcgt ccttgagaag cattgcgtct
      781 tacctgcctt gagaaagaat tttttcgagg ctctcgaggt ccccgctccc ctggcatgcc
      841 agggacttgt cgatgcgccg catcaaacca cgtaacacct tcccgggtcc gacttcgata
      901 aaggcatcga ccccgttggc gaccaggaag cggatgctgt cctcccaccg aacggaagaa
      961 tatatctgct ttttcaaaag gtcaactgtc tgttttggtt catgccagga tgccgtaaca
     1021 tttgaaacga cacgggcatg gccttcaaca aagctggcgg ccgccagatc cgctgcaaaa
     1081 ctctccgcgg ccggcttcat cagactggaa tgaaaaggac cgctgacctg gagggtgaca
     1141 aaattggttg caccggcgtc attggcaaaa gactcggcgt cagctacggc atctttatct
     1201 ccagagatga caatctgtcc gggacaattg aagttcgctg cttcgaggga tcggcctttg
     1261 gcagctgcct tgatgacttc cgagatttgt tcaggactca aacccattac ggcggccatt
     1321 ccaccccggc cctcggggtc ggactcagcc atcagttcac cacgccttgc gacaaggcgg
     1381 agggcatcct cgtaggggat catccctgcg gccaccaggg ccgaatattc acccagagaa
     1441 tggccggcgc aggcatcggg catgatttca ttggcttcga gaacgcgcag aacagcgata
     1501 cttgccgcca aaagtgctgg ttgtgtgtag cgtgtatcct tgagcttttc ttcaggtccg
     1561 gaaaagcaca gatcttccat tgagtatcct aaaacacggg aagcggtttc aaaggcttct
     1621 ctggcttcag gataggtatc atggatgtcc ttgcacatcc cgacactttg ggcaccctgg
     1681 cccgggaaaa gaaatgcaac catcaggaat ctcctttgga gaaaaaatca atagcgcaag
     1741 atgcaggcag cccatgtcaa accgccacca aaggcacaca atccgaccag ttggcctctc
     1801 ttcaagcgtc cactttcaat cacctccgcc aaagcaatgg cgattgaagc agcggatgtg
     1861 ttcccgtatt tttgcaagtt aacatagacc ttatcctcgg agaggtcaag gtatttacgg
     1921 agggactcga tgatccggag attggcctgg tgcggtatga caagatcgat cgcttgtgat
     1981 tcatacccgg cttttttgag cacggcacgc atgctgtggt tcattttcat gactgcggct
     2041 ttgaagacct cgggaccatt catcttcatg gagtgttcct gggcatcgac ggtttcatga
     2101 ctggctggtt tgcgtgtgcc acccgcaggc atgttgagaa tctcgctgta ttttccgtct
     2161 ccaccaagga agaaagaaag gaaatcgctc tccccttctt cagcggcgga aagaatggcc
     2221 gctccggcgc catcaccgaa caggacacag gtcgagcggt cagtccagtc cgtgattcgt
     2281 gagagaacct cggaggccac aaccaggaca tttttgtatt caccggttgc gatgtactgc
     2341 cgtgcaacgg agagaccgta gataaatccc gagcaggccg cttcgagatc aaaagtcgcg
     2401 gcgtgttttg cacccagcgc atgctgtacc agacaggctg tcgcgggaaa aaccatgtcg
     2461 ggagtgattg tggcaacgat aatcatatcg agatcttctg ccgtaatacc tgcagcatcc
     2521 agggccttgc gggaagcttc gatagccaga tccgaattgt tcatcccggg aggggcgatc
     2581 ctgcgttctt cgataccggt tcgggtccgg atccattcat ccgacgtatc aacccttttg
     2641 gagaggtctt cattgg
//
LOCUS       DAOSHE010000296         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37134_length_2656_cov_2.163783, whole genome shotgun sequence.
ACCESSION   DAOSHE010000296 DAOSHE010000000
VERSION     DAOSHE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37134_length_2656_cov_2.163783"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..415
                     /locus_tag="PLD82_07920"
     CDS             35..415
                     /locus_tag="PLD82_07920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG65345.1"
                     /translation="MKLDYRGHGGTIDQRKHRRATGDLMAVVGTAQGRAVQGRLKDLS
                     LSGARMEASFLLERESEVWLLFRVPHAEAVEHVLAPAKVVRLHHANEETIVYGMRFTE
                     VPERSRAVIDEYVARATGAECVCG"
     gene            406..813
                     /locus_tag="PLD82_07925"
     CDS             406..813
                     /locus_tag="PLD82_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65346.1"
                     /translation="MRINPAVVIGAVLVVVVLVVFSCSTSKSGAAPALTQEQLAEKIT
                     TLETDLKKEIAEAKSQSLILFVVGIGLGALGFLFVQHQIKRVRLEFGRRTHTVPPRAG
                     DGGSAGSTVVSKKEQPSVKKQPAAKKKAAPPKE"
     gene            complement(1092..1307)
                     /locus_tag="PLD82_07930"
     CDS             complement(1092..1307)
                     /locus_tag="PLD82_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65347.1"
                     /translation="MSLKNPHIAMATMRDLARLGLGPVLRYIPLSDGNFWSVIHCDIS
                     TDRARTQQRVLDLTRRSRWKPILSYFP"
     gene            complement(1320..>2656)
                     /locus_tag="PLD82_07935"
     CDS             complement(1320..>2656)
                     /locus_tag="PLD82_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65348.1"
                     /translation="RPDGWTSVSLHWQKRSNGGTLAPVIGWTRTTNLTIPPGKTREEF
                     TRNMAILYNPAKGMWVPLYERAQDGLRFVDICPEGTLLPITRKSESEMTVLLADGREG
                     VVSASNVRMLADNRSHADFAASLLFHAQKYIGRPVISGGLGPDGIDGSGLVHLTSRIA
                     GRLLPRDEAGISDALRAKDKTALVTGDVIMLQHAFSGQPGTALFAGPGEGIGATGTNI
                     MKFSLDDRTWLTTTWRGFPDARSPDIITVTRVTTNVTTIVATNAVTHQTNTGAGSATD
                     PGLAFATNTRPGDTTDAGSGHTNGAIIIDRSSTRTDTKLDPRTDDPDGHTSITNSDIW
                     IDRSDRADLRTTTPSTNGPAMYSIHLGSMLVAENAVRLLQAIKHTSLPVFVTVRETSQ
                     GQFLPVHAGWFADFASARQAHAANLLIQRVAPQAAVRAVPGSVLPWSAGMRL"
BASE COUNT          492 a          666 c          922 g          576 t
ORIGIN      
        1 gcataattgg tgaggagtca gtttgggagg gggcatgaag cttgactaca gggggcatgg
       61 tggaaccatt gatcagcgga agcacagacg tgcgacgggt gatctgatgg cggttgtcgg
      121 gacagcgcag gggcgtgcag tgcagggacg tctcaaggat ctcagtcttt ccggtgcgcg
      181 catggaagcc agttttttgc tggaacggga gagtgaggtc tggctactct tcagggtgcc
      241 gcacgccgag gcagttgagc acgtgcttgc gccggccaag gttgtccggc tgcaccatgc
      301 aaacgaagaa accattgtat acgggatgcg ctttaccgag gttccggagc gcagccgggc
      361 agtcatagat gaatatgtgg ctcgggccac gggagcggag tgtgtatgcg gataaatccg
      421 gctgttgtca ttggagcggt tctggttgtt gttgttttgg tcgtgttttc ctgttcgacc
      481 tccaaaagcg gtgctgctcc tgcgttgacg caggaacagc tcgccgaaaa aatcacgacg
      541 cttgaaaccg atctgaaaaa ggagattgcc gaggcaaaaa gccaaagttt gattcttttt
      601 gttgtgggaa tcggcctcgg ggccttgggt tttctgtttg tccagcacca gatcaagcgg
      661 gtgaggcttg aattcgggag aaggacgcat accgttcctc cccgtgccgg ggatggtgga
      721 tctgccgggt caaccgtcgt ttcaaagaag gagcagccgt ctgtcaaaaa gcagccagct
      781 gcgaagaaaa aggcggcacc gcccaaagag tgacggagcc gttccggtac cggcccgcgg
      841 attgtgtcag ggtaagcgat ggtctttcat ccggcgctgt ccgcggtttt tttgttttcg
      901 gatccgggaa cggtgtggtg ttcctgatgt gtattaagcg cacaccaagc tgtagagaag
      961 ctatttggca gggggcaaag agaagcgcgg tccttggctg tcgcccaggg cgtttggtct
     1021 gacgggctgg ctctccgggc agctgcgtgg ccaccgggag agccccgttg ccttgggatg
     1081 aatatccttg ctcacgggaa gtacgagagg atcggtttcc agcgtgagcg gcgtgtcagg
     1141 tcgagtaccc gttgctgtgt gcgggctctg tccgtactga tgtcgcagtg gatcaccgac
     1201 cagaagtttc cgtccgagag cgggatgtag cgcaggacgg gccccagacc gagacgggcc
     1261 agatcgcgca tggtcgccat tgcgatatgg ggattcttga gcgacatgac ctggacactt
     1321 tagagacgca ttccggccga ccagggaaga acactgcccg gaacagcccg gacagcggcc
     1381 tgaggcgcga cgcgctggat caggaggttg gctgcatgcg cctggcgggc cgaggcgaag
     1441 tccgcaaacc agccagcgtg gaccggaaga aattgtccct gagaagtttc ccgaacggtg
     1501 acaaagaccg gcagtgaggt gtgtttgatg gcctgcagaa ggcggacggc attctccgcg
     1561 acgagcatgg atccaagatg gatggaatac atggccgggc cgtttgtgga gggtgtggtc
     1621 gtcctcaaat cggcgcgatc gctgcgatcg atccagatgt cggaattggt tattgaggtg
     1681 tgcccatccg gatcatcagt ccggggatcc agtttggtgt cagtgcgggt gctgctgcgg
     1741 tcaatgatga tggcgccgtt cgtgtgtccg gaaccggcat ctgtcgtatc acccgggcgt
     1801 gtattggtcg cgaaggcgag tcccgggtct gttgctgatc ccgcaccggt attcgtctgg
     1861 tgtgtgacgg catttgttgc aacgatcgtt gtgacgttgg ttgtgacacg ggtcaccgtg
     1921 atgatgtcgg ggctgcgtgc gtccgggaat ccccgccatg tggtcgtcag ccatgtgcga
     1981 tcgtccaatg aaaatttcat gatgtttgtt ccggtggccc cgattccctc gccgggtccg
     2041 gcgaagaggg cggtcccggg ttggccggag aaggcgtgtt gcagcatgat gacgtcgccc
     2101 gtcacaagtg cggtcttgtc cttggcacgc agggcgtcgg agatgccggc ttcatcgcgg
     2161 gggaggagtc gtccggcgat acggcttgtc aggtggacca gtccgcttcc gtcgatgccg
     2221 tccggcccga gtccgccact gatcacaggc cggccgatgt atttctgcgc gtgaaagagg
     2281 agggatgcgg caaaatcggc atggcttcgg ttgtcggcga gcatccggac attggaagcg
     2341 ctgacaacgc cctcgcggcc gtcggcaaga agaacagtca tttcgctctc ggatttcctg
     2401 gtgatcggga ggagggtccc ttcggggcag atatccacaa agcgcaggcc gtcctgggcc
     2461 cgttcataga ggggaaccca catgcccttg gcggggttgt agaggatcgc catattcctg
     2521 gtgaattcct cccgtgtctt cccggggggg atcgtcaggt tggtggttct ggtccagccg
     2581 atgaccgggg ccagggttcc gccattggaa cgtttttgcc agtggaggga gacgctcgtc
     2641 cagccatcgg ggcggt
//
LOCUS       DAOSHE010000297         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37348_length_2645_cov_3.037838, whole genome shotgun sequence.
ACCESSION   DAOSHE010000297 DAOSHE010000000
VERSION     DAOSHE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37348_length_2645_cov_3.037838"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLD82_07940"
     CDS             <1..1243
                     /locus_tag="PLD82_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG65349.1"
                     /translation="DGLDAIEHIQKIEYPLILLDLRMPRMTGIEVLQYLHDSQSRAVP
                     IVITANNDVESAVQSMRLGAYDYIVKPFDNERLLVTVRNALEKKRLENEVHHLREEAG
                     TTGIDGIIAESSAMKKVFSLVHRVLDNDVTLLITGESGTGKEVFARAVHNNSTRKSGP
                     FIALDCASIPETLIENEFFGHEKGAFTGAMTAVPGKFELADGGTLFLDEIGNLRLDVQ
                     AKLLRVLQEREFSRIGGTRRIQVDVRIICATNADLELAMRENRFREDLYYRIHVVPIK
                     LPALREREGDVPRLIRHFLQSFNAKMGKSIVVSERAMLKLCQYSWPGNVRELENMIHR
                     LVVIKDSDVIEADDLPHEIRHPEKALVSGFSSSMTLEEVERQHIKKILHEEGNNISRA
                     AQVLGITRKTLHAKLAKLHLE"
     gene            complement(1260..1949)
                     /locus_tag="PLD82_07945"
     CDS             complement(1260..1949)
                     /locus_tag="PLD82_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG65350.1"
                     /translation="MNTRLRTSAWLETEAGSILIDCGPDFRQQALQHGISEISAIVLT
                     HHHADHIGGLSELRPLSRLHPVRVYASGHTCTIVQNTFSYIFSEKPTGGGLPRIDLQT
                     FNGSWNINGLCITPLPVLHGEDRIHGFRIGDMAYITDASFIPESTFELLSGIRYLVIN
                     GLREKPHATHFSIAQACHAAARTSAQHVWITHICHLVDHAAISTSLPPPVSPGYDGLR
                     FEFDENAGARQ"
     gene            2154..>2645
                     /locus_tag="PLD82_07950"
     CDS             2154..>2645
                     /locus_tag="PLD82_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG65351.1"
                     /translation="MDKHTAVTSEGLEFLAGKARQAALQLQDIAHDHRVEALRSMARA
                     IRVHSAEILRENALDCLDAKASGLGMSLYKRLELDEAKIEQMAVGLESVTALPDPVGV
                     LQEKIELDSGLVLEKVSCPLGVLAIIFESRPDVVPQVVGLSVKSGNAVIFKGGSEALR
                     SNRV"
BASE COUNT          632 a          650 c          756 g          607 t
ORIGIN      
        1 agatggtctt gatgcgattg agcatatcca gaaaatcgag tatcccctga ttctccttga
       61 tctccggatg ccccgcatga cagggatcga ggtccttcag tatcttcatg attcgcagtc
      121 cagggccgtc ccgatcgtga tcaccgcaaa caatgatgtc gagagtgcag tccagagcat
      181 gcgactcggt gcgtatgact atatcgtcaa accattcgac aacgagcggc tcctggtgac
      241 tgtccggaac gcactcgaaa aaaaacgtct cgaaaacgag gttcatcatc tcagggaaga
      301 ggccgggacg accggtattg acggaatcat tgccgagagt tcggcaatga aaaaagtctt
      361 cagcctggtc catcgcgtcc ttgacaatga cgtgacactc ctgatcacgg gcgagagtgg
      421 aaccggaaaa gaagtcttcg cgagggctgt tcacaacaac agcacccgca agtccggtcc
      481 cttcatagcc cttgattgtg cgtccattcc cgaaaccctg atcgaaaacg aattctttgg
      541 acatgagaag ggcgccttta caggtgcgat gaccgctgtt cccggcaagt tcgaactggc
      601 tgatggcgga acactttttc ttgacgagat cgggaatttg cgactggatg tccaggccaa
      661 gctgttgcgt gttttgcagg aaagggaatt ctcccggatt ggaggcaccc gacggattca
      721 ggtggatgtc cggatcatct gtgccaccaa tgcggatctc gaactggcca tgagagaaaa
      781 ccggttcagg gaggatctct attacagaat ccatgttgta ccgatcaagc ttccggcact
      841 gcgggagcgg gagggcgatg ttccacgctt gatcagacat tttcttcaaa gcttcaatgc
      901 caagatggga aaatcaatcg ttgtttcgga gcgtgccatg ctcaagctct gccagtattc
      961 ctggccgggg aatgtccgtg aacttgaaaa catgattcat cggctggttg tcatcaagga
     1021 cagcgatgtc atcgaagccg atgatcttcc ccatgagatc cgtcatccgg agaaggcact
     1081 tgtcagtgga ttttcctcca gcatgactct ggaagaggtt gagcgacagc atattaaaaa
     1141 aatccttcac gaagagggta ataatatcag ccgggccgcc caagtgctcg ggattaccag
     1201 aaaaacactt catgccaagc ttgccaagtt gcatctcgaa tgaggaccgg tttctccgat
     1261 tactgccttg cgccggcatt ctcgtcaaat tcgaaacgaa gaccgtcata acccggagag
     1321 accggtggcg ggagcgaggt cgagatcgcg gcatgatcca ccaggtggca gatgtgtgtt
     1381 atccagacat gctgagcgga ggtacgggct gcggcatgac aggcctgggc gatcgagaag
     1441 tgggttgcat gtggtttctc gcgcagtccg ttgatcacga ggtaccggat gccggagagc
     1501 agttcgaatg tggattcagg aatgaaggat gcatcggtga tgtatgccat atccccgatc
     1561 ctgaaaccgt ggatccggtc ttctccatgc aggacaggaa ggggtgtgat gcaaaggcca
     1621 ttgatgttcc atgatccgtt gaaggtctgc agatcgattc ttggaagtcc gcctcccgtc
     1681 ggtttttcgg aaaaaatata cgaaaacgta ttttgaacaa tcgtgcaggt atgaccggat
     1741 gcatagactc ggacgggatg caaacgtgag agtggcctca gttcggaaag tcctccgata
     1801 tggtcggcat gatggtgcgt gagcacgatt gccgagattt cggatatccc gtgttgcaac
     1861 gcctgctgac ggaagtccgg gccgcaatcg atcaggatcg aaccagcttc ggtctcgagc
     1921 caggcacttg tgcgcaatct tgtgttcatg ggatctcgtg aagtacagac aggacaggaa
     1981 catcccagca tggggattcc gtccgatgtt ccggtaccga gcagggtgag acgtgccatt
     2041 caccaaaaat acgggtattt gcgcagatgg acgtgattct ttgcgggcgg gaatgcgtcc
     2101 tggtcgcgga cgaaaagtgg tactttgctg tcgaaagaga gtaatttatc gttatggaca
     2161 aacacactgc ggttacatcc gaaggacttg aatttctggc cggcaaagcc aggcaagctg
     2221 ctttacagct tcaggatatt gctcatgatc atcgggtaga ggcacttcgc tcgatggccc
     2281 gggcgatccg agtgcattct gccgagatcc ttcgcgaaaa tgcgctcgat tgtcttgatg
     2341 caaaagcttc aggtctcgga atgagtttgt acaagcggct tgagctcgac gaagccaaaa
     2401 tcgaacagat ggcggtcgga cttgaaagtg tgaccgcctt gcccgatccg gtcggggttc
     2461 tccaggagaa gatcgagctg gattccggat tggttctcga aaaagtctcc tgcccccttg
     2521 gcgtcctggc catcattttc gagtcacgac cggacgttgt tccccaggtt gtcggactct
     2581 ccgtcaaatc cgggaatgcc gtcattttca aaggcggcag tgaggccctg cgttcaaacc
     2641 gtgtg
//
LOCUS       DAOSHE010000298         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37399_length_2643_cov_2.810665, whole genome shotgun sequence.
ACCESSION   DAOSHE010000298 DAOSHE010000000
VERSION     DAOSHE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37399_length_2643_cov_2.810665"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2522
                     /gene="uvrA"
                     /locus_tag="PLD82_07955"
     CDS             <1..2522
                     /gene="uvrA"
                     /locus_tag="PLD82_07955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG65352.1"
                     /translation="LLYARIGVPYCPSCGKEITSQSIDQIVSEILLRESGKIMVLAPI
                     ARGAKGEYKKEFAKMIQEGFVRARVDGEVVEISEGMSLKKTFKHTIEIVVDRIVLRDT
                     VKSRLSESVETALKYGDGLVVIHDVDKGEDTTYSEKHACPDCGFTIDEIEPRLFSFNN
                     PFGACPECTGLGSRMEFDPELVIPDRSLSLNQDAIRTIGSMQGSWITTQLRALSEAFG
                     FSLDEPVSSMSKEALDVLLFGSKGREFHYVYSGKQGKSRYEFTGRFEGLIPNLERRYH
                     ETKSEGMREWMEKFMRQQPCNTCHGQRLKPAALAVRVGGKNIIELTSLDVGASLAFFD
                     ALELDPRRAAIAKQIIKEIRERLGFMLDVGLDYLSLDRMAGTLSGGEAQRIRLATQIG
                     SSLMGVLYVLDEPTIGLHQRDNDRLLSTLRRLQKLGNTLVVVEHDEQTIRQADWLIDL
                     GPGAGEHGGNVIFSGPPDGILDNPHSLTGQYLSGRLSIGVPATRRPGNGLFLVVRGAR
                     ENNLKSIDVSFPLGCLVAVTGVSGSGKSSLVSEVLSKGVAHALGLEREIPGKHDRIEG
                     LEHIDKLINIDQSPIGRTPRSNPATYTGLFTPIRDLFASLPEAKARGFAPGRFSFNVK
                     GGRCETCEGAGVLKIEMHFLPDVYVTCDVCHGRRYNSETLEVRYKGKNIHEVLDMTIE
                     EALDFFNAFPAMNSKLQTLVDVGLGYVRLGQPATTLSGGEAQRVKLATWLSRRSTGKT
                     LYVLDEPTTGLHFDDVRRLLAVLERFADAGDSVIVIEHNLDVIKRADWIIDMGPEGGR
                     RGGTVVVSGTPEDVAACPGSHTGRYLAPLLSGPQRRTRKKN"
BASE COUNT          532 a          778 c          815 g          518 t
ORIGIN      
        1 gactcctcta tgcacggatc ggggttccct attgtccctc ctgcgggaag gagatcacca
       61 gccagtccat cgaccagatc gtgagcgaga tcctcctcag ggagtccgga aagatcatgg
      121 tcctggcccc gattgcccgt ggggccaagg gtgaatacaa aaaagaattt gcaaaaatga
      181 tccaggaagg atttgtccgg gcccgggtgg acggggaagt cgtggagatc agcgagggga
      241 tgtccctcaa aaagacgttc aaacatacga tcgagattgt cgtcgaccgg atagtccttc
      301 gcgatacggt caagagccgg ctttccgaat cggtcgagac ggctctcaag tacggggacg
      361 ggctggttgt tatccacgat gtcgacaagg gcgaggacac gacctattcc gaaaaacacg
      421 cctgtccgga ctgcggattc accattgacg aaatcgaacc ccggcttttt tcgttcaaca
      481 atcccttcgg agcttgcccc gagtgtacgg gactcgggag ccggatggag ttcgacccgg
      541 aactcgtcat cccggacagg agtctcagtc tcaaccaaga cgcgatccgc accatcgggt
      601 cgatgcaagg ctcctggatc acgacccagt tgcgtgcgct ttcggaggct ttcgggtttt
      661 ccttggacga acctgtctcc agcatgtcca aagaggccct ggatgtcctc ctcttcggat
      721 ccaaggggcg cgagttccac tacgtctaca gcgggaagca gggcaagtcg cggtacgagt
      781 ttaccggacg cttcgagggg ctgatcccca atctcgaacg gcgctatcac gagaccaagt
      841 ccgagggcat gcgcgaatgg atggaaaaat tcatgcgcca gcaaccctgc aatacctgcc
      901 atggccaacg cctcaaaccg gcggccctgg cggtcagggt cggcgggaaa aatatcatcg
      961 agctgacttc tctggatgtc ggtgcgagcc tggccttctt tgatgcgctc gaactcgatc
     1021 cgagacgtgc tgcaatcgca aaacagatca tcaaggaaat ccgcgagcgg ctcggattca
     1081 tgctcgatgt cggactggac tacctctcgc ttgaccggat ggcgggcacc ctctccggag
     1141 gtgaggcgca gcggatccgg ctggccaccc agatcggctc gagcctgatg ggggtcctct
     1201 acgtccttga tgagccgacc atcggcctgc atcagcggga taatgaccgg ctcctttcca
     1261 cgctgcgccg tttgcagaag ctgggcaaca ccctggtggt ggtcgaacac gacgaacaga
     1321 caatccgcca ggcggactgg ttgatcgatc tcggaccggg ggccggcgaa cacggcggga
     1381 acgtgatctt ttccggtccg cccgacggga tcctcgacaa tccgcactcc ctgaccgggc
     1441 aatatctttc gggccggctc tcgatcggtg ttccggcaac gcgcaggccg gggaacgggc
     1501 tcttcctggt ggttcgcggc gcccgggaga acaatctcaa atcgatcgat gtcagcttcc
     1561 ccctgggctg tctggtcgcc gtgacgggcg tctccggttc gggcaagtcc agtcttgtga
     1621 gcgaggtcct ctccaaaggg gtggcccacg ctctcgggct ggaacgtgaa attccgggga
     1681 agcatgaccg gatcgagggc ctcgagcata tcgacaagct gatcaatatc gaccagtccc
     1741 ccatcggccg gacgccacgg tccaatccgg ccacctatac cgggctcttc acgccgatca
     1801 gggatctctt tgccagcctg cctgaagcca aggcccgtgg atttgctccg ggacggttct
     1861 ccttcaatgt caagggcggg cgctgcgaaa cctgcgaggg ggccggcgtt ctcaagatcg
     1921 agatgcattt cctaccggat gtctatgtga cctgcgatgt ctgccacggc cgccgctaca
     1981 acagcgagac cctcgaggtg cgctacaagg gcaagaatat ccacgaagtc ctggacatga
     2041 cgatcgagga ggcgctcgat ttcttcaatg ccttcccggc gatgaactct aaactccaga
     2101 cactggtgga tgtcggattg ggctatgtcc ggctgggcca gccggcaacg accctctccg
     2161 ggggagaggc ccagcgcgtc aagctggcaa cctggctttc cagacggtcc acagggaaga
     2221 cgctttatgt gctggacgag ccgacgaccg ggctgcactt cgatgatgtc aggcgcctgt
     2281 tggccgtcct tgagcgcttt gccgatgccg gtgattcggt gatcgtgatc gagcacaatc
     2341 tcgacgtgat caagcgtgcc gactggatca tcgacatggg gccggagggc ggccggcgcg
     2401 gggggacggt tgttgtttcg ggaacacccg aggacgtggc cgcctgtccc gggtcgcata
     2461 ccgggcgcta cctggccccg ctcctgtccg gtccgcagcg caggacccgc aaaaaaaact
     2521 gacctgggat gtaccccgtt ttgggcttgt gcgagaatgt ggacggcaga aactgattct
     2581 ggcgatattt tgtatacata cggatacgga ctgtgatcgg gcgttgcgga taccagtcca
     2641 tct
//
LOCUS       DAOSHE010000299         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37462_length_2640_cov_3.789942, whole genome shotgun sequence.
ACCESSION   DAOSHE010000299 DAOSHE010000000
VERSION     DAOSHE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37462_length_2640_cov_3.789942"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..537)
                     /locus_tag="PLD82_07960"
     CDS             complement(70..537)
                     /locus_tag="PLD82_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65353.1"
                     /translation="MERSQLPEVIFERAKTLPVPLIVWDRDSSLLIVSLTEENEYSGE
                     FINTGRVCIALGDLMQAVDIEVNYSPADVTVDPDLAMPEAFAASVRFTDSYLIVDTIS
                     QIKVLPDFSLMHLWYTDAPPSEMLHLRIAEHVIFDVTIDNRLAGIWVGNIVQR"
     gene            885..>2640
                     /locus_tag="PLD82_07965"
     CDS             885..>2640
                     /locus_tag="PLD82_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG65354.1"
                     /translation="MPESTAPNQAYVRQRERWMALRNKRGGHPPKVRLGLRKKLFFLI
                     VALISGLLAVVYTMTLSDEERVLRRELKIRGGTVARNLADSIYNSYANLVQDRMAKAD
                     DPFVFNPDEPSGFEPLAFSDAMKKAGEQQDFVAGYAIDPWNRAFVHSARKGYLYPLPT
                     PDGIFDFKAIYPVKLALMARIEREGLPVQDVTGPDLQQKLCSSIPVGTNAGLLLEPVR
                     EKLKVYLVSVSNTLFLQTVSETGAARRIPLAIATSNLPALAGLALTILREEYDESVLL
                     LMNTANELRRIRRQVTAMADKDRLAAVFSGLERLVERGTLFRNALSGGLISQYSNAIS
                     EISDGAARTNAQRILSQAGTAERRFEPFVQVYQPSASGETLLYINYPMCETDKYFSTY
                     RGEVHLLLSQTGITYTINYAKEKLQLAALLAIAAGTFFTLVMAFFITSPIKKLMGAMR
                     RVGVGDLEQIVLVRVKDEIGVLSEHFNEMTEGLREKEKIRSAMNKAVSKEIAEVMLSG
                     ELKLGGDKKIVTVLFSDIRGFTTISEAHTPEEVVELLNEYLTLMTNIVEKHRGNVDKF
                     VGDEIMALWGAPKSYGNDA"
BASE COUNT          591 a          812 c          710 g          527 t
ORIGIN      
        1 agaagaagcc cgatcgatcg gatacggtga tgcacctatt ccccctgtct tgcaacgaca
       61 agccgcctgt caacgctgga cgatatttcc gacccagatt ccggcgagcc tgttgtcgat
      121 agtcacatca aaaatgacat gttcggcgat ccggagatgc agcatctcgc tgggtggtgc
      181 atcggtgtac caaagatgca tcagcgagaa atccggcagc accttgatct ggctgatcgt
      241 gtccacaatc aggtagctgt cggtgaaccg taccgaagca gcaaaggcct ccggcatggc
      301 gagatctgga tcgacagtga catcggccgg agagtaattg acctcgatgt cgaccgcctg
      361 catcaaatca ccaagtgcga tgcagacccg gccggtattg atgaattcgc ccgagtattc
      421 gttttcttcg gtgagactga cgatcaggag actactgtcc ctgtcccaga cgatcaacgg
      481 gaccggaagg gtctttgccc gttcaaaaat gacctcgggc agttgcgagc gttccatgca
      541 tccccctgtc ggtgttccgc aaaaaggtag ccaaaagttc gtaccacggc aagcaggcgc
      601 cgacggcttt tctgtttgat gaacaccctc gcgcccacgc aaagcgtatc gatcgacggg
      661 ttctctgcag gccgagtcct ccgtccaagc tggcttcccc gcagagatgg ttgcgttggc
      721 gatggagtgg ccctgtctct ttcagcccgt tgatcttcgc agatggtttg cctgccatcg
      781 gccggtgaga caaccgtttt tgtgcatccc ggattccggc ctcttggcaa acggccagta
      841 acggtgtagg atggaagaac cttgacaaga aggggctcct tcccatgccc gagagcaccg
      901 cacccaacca ggcctatgtc cgtcagcgcg aacgctggat ggccctgcgc aacaaacggg
      961 gtggccatcc cccgaaggtc cgcctgggcc tgcgaaaaaa gctctttttc ctgatcgttg
     1021 ccctgatctc cggactcctg gccgttgtct acacgatgac gctctccgac gaagaacggg
     1081 tgctgcgccg ggaactgaag attcgtggag gcaccgtggc ccgcaatctc gcggactcca
     1141 tctacaacag ttacgccaac ctcgtccagg accggatggc caaagccgac gaccccttcg
     1201 tgttcaatcc cgatgaaccc tccggctttg agcccttggc cttctcggac gccatgaaga
     1261 aggccggtga acaacaggac ttcgttgccg ggtacgccat cgatccctgg aaccgggcct
     1321 tcgtccattc ggcccgcaaa ggctatctct atcccctccc gacaccggac ggaatcttcg
     1381 atttcaaggc gatctacccc gtcaaactgg cccttatggc ccggatcgag cgggagggac
     1441 ttccggtaca agacgtcacc ggtccggatc tccagcagaa actctgttca tccatcccag
     1501 tcgggaccaa tgccggcctg cttctcgagc cggtcaggga aaaactcaag gtctatttgg
     1561 tttccgtcag caataccctc tttctccaga cggtgtcgga gacaggagcc gcaagacgca
     1621 tccccctcgc catcgcgacc agcaacctcc ccgcgctggc cggcctggcg ctgaccatcc
     1681 tccgcgagga atatgatgaa tccgtcctgc tcctgatgaa taccgccaat gaactccggc
     1741 gcatcagacg gcaggtcacg gccatggccg acaaggatcg acttgccgcc gtcttttcgg
     1801 gactggagag attggtcgaa cggggaaccc tcttcaggaa cgccctgagc ggaggactca
     1861 tctcgcaata ctccaacgcc atcagtgaaa tctcggacgg agcggcccgc accaacgccc
     1921 aacgcatcct gagccaggcg ggaaccgcgg agcgccgctt tgaacccttt gtccaggtct
     1981 accaaccctc cgcgagtggc gagaccctgc tctacatcaa ctatccgatg tgcgaaaccg
     2041 acaagtattt ttcaacctac cgtggtgaag tccatctcct cctctcgcag acaggaatca
     2101 cctataccat caattacgcc aaggaaaaac tccagctggc ggccctcctc gccattgcgg
     2161 caggaacctt tttcacactc gtcatggcct ttttcatcac aagcccgatc aaaaagttga
     2221 tgggcgccat gcgcagggtc ggggtgggcg atctcgagca gatcgttctc gtccgtgtca
     2281 aagacgagat cggggttctc tccgaacact tcaacgagat gaccgaaggc ttgcgcgaaa
     2341 aggagaagat ccgcagcgcg atgaacaagg ccgtttccaa ggaaatcgcc gaagtcatgc
     2401 tctccggaga actcaaactg ggcggtgaca aaaaaatagt cacggtcctg ttctctgaca
     2461 tccgggggtt tacaacaatc tcggaagcgc acacaccgga agaggtggtc gagcttctca
     2521 acgagtatct gaccctgatg accaatattg tcgaaaagca ccgggggaat gtggacaaat
     2581 tcgtcggtga cgagatcatg gccctctggg gtgctcccaa gtcctacggc aatgacgcca
//
LOCUS       DAOSHE010000300         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37586_length_2635_cov_2.201550, whole genome shotgun sequence.
ACCESSION   DAOSHE010000300 DAOSHE010000000
VERSION     DAOSHE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37586_length_2635_cov_2.201550"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1501)
                     /locus_tag="PLD82_07970"
     CDS             complement(77..1501)
                     /locus_tag="PLD82_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG65355.1"
                     /translation="MASTMPGLTTYTEPFEDYNGASYATGTFALTTGSWYVVAAFKDS
                     ATHGGSVSVRLNDDVAGSCIVSPVMNGLGSVSFWYRALNAESADSTFKVQTSVNGGTT
                     WSDAGSGTFKSQTWAQYSCTINSTAGSIQFRVLNDNQAGHLILGDLVFTSYNVTWGSV
                     SSSASAVSSATSSVSSAGFSSSSSSSSLGTTSSAGGGTTFAEDFTLPQTVRDYYRSAY
                     GKSGSALKTALKTIITHTYTSYDGLKTLYATSDVRADGKIWDMYSTHADGTSHDGEWG
                     VLGNATYFTTTKTYYSYTTSDAGASSSTEGTGGWNREHTWPKSNFGGSTSNQPGTDAH
                     HITPTDVKVNGWRSNYPYGEVDSSAPYSVNGSRLGSARSGLGYSGTVFEPIDAYKGDH
                     ARMHFYMSVRYYNDSLFQDGDWSYAGAKLKPWYDAMMRNWNASDPVSAKEIARNNAIY
                     NGNQKNRNPFIDYPALVTLIDFQN"
     gene            1491..1820
                     /locus_tag="PLD82_07975"
     CDS             1491..1820
                     /locus_tag="PLD82_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65356.1"
                     /translation="MLATLLDTAGLLDTAGLLDTAGLLDTAGLLEMNPVDEALPVETA
                     EVLAEDIEAEDTVSVEEAADAERLLEPDDTPTTGTTTSSSPQPTPKERAATTANPVTT
                     RTPKCMH"
     gene            complement(2047..2466)
                     /locus_tag="PLD82_07980"
     CDS             complement(2047..2466)
                     /locus_tag="PLD82_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65357.1"
                     /translation="MKKIVLFVGVCVLSLAMLAGCGKKDDKDAMFGTTDQDGKKAAIS
                     KEKFMEYLTKKLAIVGTPEYADAMTKYKAEAKDVKEILDRQKIFDKYFGDYKKKEADL
                     LNSCGLTLGDVVTASTSFKSDKEVMDLVYKLMKYVVY"
BASE COUNT          638 a          738 c          660 g          599 t
ORIGIN      
        1 tgccggttca tgcaaaagcc ccgtcctctg ccgtgcggca cgagacgggg ctgtacagat
       61 gctgtcttcg atactatcag ttctgaaaat cgatcaaagt gaccagtgcc ggatagtcaa
      121 taaagggatt gcgatttttc tggttcccgt tatagatcgc attattcctg gcgatttcct
      181 tggcactgac cggatcactg gcgttccagt tccgcatcat ggcatcatac cagggcttga
      241 gtttggcccc ggcataggac caatcgccat cctggaaaag agaatcattg tagtaccgga
      301 ctgacatgta aaaatgcatt cgggcatggt cgcccttgta ggcatcaatc ggttcaaaaa
      361 cggtcccgct atacccaagc ccgctccggg ctgacccgag acggctccca ttgacactgt
      421 atggcgctga cgaatcaacc tcgccgtacg ggtaattgct gcgccaccca ttcaccttga
      481 catcggtggg agtgatatgg tgggcatcgg ttcccggctg attgctggtg ctgccaccga
      541 aattgctctt gggccaggtg tgctcacggt tccagcctcc ggtcccctct gtcgaggaag
      601 atgcgccagc atccgaagtc gtgtagctgt aatacgtttt tgttgtcgta aaatacgtcg
      661 cgttcccgag gacaccccac tcaccgtcat ggctcgttcc atcggcatgc gtcgaataca
      721 tgtcccagat cttgccgtca gcccggacat ccgaggtggc atacaaggtc ttgagcccgt
      781 cgtaactggt ataggtatgc gtaatgatgg tcttgagcgc cgtcttgagg gccgatccgc
      841 tcttgccata tgcactgcga tagtagtcac gcactgtttg cggcagcgta aaatcttcgg
      901 caaaggtcgt cccgccacca gcgctggagg ttgttcccag tgaagaagaa cttgaagaag
      961 aggagaagcc cgcagacgac acggaggagg ttgccgagga aaccgcgctg gctgacgatg
     1021 agacagatcc ccaggtaaca ttgtagctgg tgaacacaag atcgcccaag atcaaatggc
     1081 ctgcctgatt gtcattgagc accctgaact ggatgcttcc cgccgtcgag ttgatggtac
     1141 aggaatattg ggcccatgtc tgggatttga acgttccgct gccggcatcg gaccaggtgg
     1201 tgccaccatt gaccgaggtc tggactttga acgtcgaatc cgctgactcg gcattgagcg
     1261 cccggtacca gaaagagacc gatccaagcc cgttcatcac cggagaaaca atgcagctcc
     1321 cggcaacatc atcgttcaag cggacagaaa cggatccgcc atgggttgct gaatccttaa
     1381 acgcagccac cacgtaccag ctcccggttg tgagagcaaa ggtcccggtc gcataggaag
     1441 caccattata gtcttcgaaa ggctcagtat aggtcgtcag tccgggcata gtgctggcca
     1501 cactgctcga caccgcaggg ctgctcgaca ccgcagggct gctcgacacc gcagggctgc
     1561 tcgacaccgc gggactactg gagatgaacc ctgttgatga agcgctcccg gtggaaacgg
     1621 ctgaagtact ggctgaagat atcgaagcgg aagacacagt atctgttgag gaggcagcag
     1681 atgcggaaag gctgcttgaa ccggatgaca cacccactac cggtacaaca acatcatcgt
     1741 caccacaacc gacaccgaaa gaaagggcgg ccacgacggc caacccggtt acaacacgta
     1801 cgcccaaatg catgcattga ctcctgtttg tatctctccg ccggaacatg gcgcggagga
     1861 cctctcctga tccttcacac caaggatgta tccagaaccg ggaacaagtc aaattcaatc
     1921 gtttccccac gtctcttcga gcaacacaca gatcggccca tacacgggga tcacgcaacg
     1981 ataccccaaa aatacaacaa gcgaccggat ggccgcttgt tcggataccg gctgtttcga
     2041 tccggatcaa tacacaacgt acttcataag tttgtacacg aggtccatca cttccttgtc
     2101 acttttgaac gatgtactcg cagtcacaac atccccaaga gtcaggccac aagaattaag
     2161 gagatctgcc tctttctttt tgtaatctcc aaaatatttg tcaaagatct tttggcgatc
     2221 cagaatttct ttgacatcct tggcctcagc cttgtacttg gtcatggcat ccgcatactc
     2281 gggtgtcccg acgatggcca gtttttttgt gagatactcc ataaattttt ctttggagat
     2341 cgccgccttt ttaccatcct gatcggttgt cccaaacatc gcatccttgt catccttctt
     2401 tccacatccg gccagcatgg ccaaagaaag cacacagact ccaacaaaca gcacaatctt
     2461 tttcatcatg gcacccctct gcgcaagttt ttgaatacaa catccccaaa gtatcacgtt
     2521 tcaaattttt ttctgcttgg tgtggcaatc tctcgcaccg gatcgatgca cctaagtgat
     2581 agcagacaag tttttataga agaaaacgag aggtcagtca ggcgtgcaat cccgg
//
LOCUS       DAOSHE010000301         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_37960_length_2617_cov_3.822795, whole genome shotgun sequence.
ACCESSION   DAOSHE010000301 DAOSHE010000000
VERSION     DAOSHE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37960_length_2617_cov_3.822795"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..699
                     /locus_tag="PLD82_07985"
     CDS             256..699
                     /locus_tag="PLD82_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65358.1"
                     /translation="MALDDAAVELDTALDEAAAELDTALDEAAAELETALDEAAAELE
                     TALDEAAAELDTALDEAAAELEVAFVAELDETFVVDDVDVAWDEASVGWLDAGRTGTA
                     SSSLQAGKAIKSVRSMAAKASHASGCRFMRSVPPSGSCCVARIQF"
     gene            complement(804..1100)
                     /locus_tag="PLD82_07990"
     CDS             complement(804..1100)
                     /locus_tag="PLD82_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG65359.1"
                     /translation="MRDQSINLAMDEQRGWIESQVGLNQNLTDRVEQLPISKREKEVV
                     NLVLAGKNRSDIAEALFISDETVKQHLTNIYRKLEIRGKSGLFSLVYGPHEKTP"
     gene            complement(1131..1952)
                     /locus_tag="PLD82_07995"
     CDS             complement(1131..1952)
                     /locus_tag="PLD82_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65360.1"
                     /translation="MPNDRHLQSITIGIFVFMSLVSLAMIRNDLTDISDPTLCSIIIV
                     FRILFSGFILASCVIYIKSGRSRLFSNLVQLIISMTALISGVITWTRPPDSSVSFYIT
                     TLVIIAAWAVPTRGILSRAMPLLALLANDVTALLVQRSLESRTTLTIIVTLVSSDLIG
                     LWVAWRLGSLRALEQKLIDTHRREAEFRRMIADVLLDGVIVSRNRIIIDCNQTLCQML
                     DRKKNDLCGEEVQSILTSHPDDGEAVFDNMENRPRMMTLRSSIGDLPVEVSHRSV"
     gene            complement(2385..>2617)
                     /locus_tag="PLD82_08000"
     CDS             complement(2385..>2617)
                     /locus_tag="PLD82_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOG65361.1"
                     /translation="AFLAAGFSMLRSRAGLAAGFGAILLYASDAVIGIGRFTPVLPHG
                     PLFEVLVLAPYFGGLALFVTGSIRLTRESAAE"
BASE COUNT          553 a          646 c          868 g          550 t
ORIGIN      
        1 gcgctcttcc aaaggacttc cttggttgtc gcgttgatct tgtacatata atagtcgccc
       61 ggatcgagag gaacggcgat ctccagcgtg gccgggtcga attggcgggt catggtgctg
      121 ccttcggtaa aggatgcagt tgcgccgacg gatgtcccgt ccggggccag ccatatttct
      181 tcacctgcgc tgaaaggagg tgtcgtgatg gtcgttccgg gctgagcctc ctggcttgag
      241 gcgattggtt cggagatggc gctggatgat gctgcggtcg aactggacac cgcgctggat
      301 gaagcggccg ccgaactgga caccgcgctg gatgaagcgg ctgccgagct tgagacggcg
      361 ctggatgaag cggccgccga gcttgagacg gcgctggatg aagcggccgc cgaactggac
      421 accgcgctgg atgaagcggc cgccgaactg gaggttgcgt ttgtcgcaga actggatgag
      481 acgttcgttg tcgacgatgt ggatgtagcc tgggatgagg ccagtgtggg ttggctggat
      541 gccggcagga cgggcacggc ttcatcgtca ctgcaggcag gcaaggccat aaagagcgtg
      601 aggagcatgg cggccaaggc cagccatgca agcgggtgtc gtttcatgag gagtgttcct
      661 ccgtctggaa gttgttgtgt cgcccggatc cagttttgac gttagggcat aaaaaaacaa
      721 tcccccggac gggggatttt tagcatcatg cagccatgat gggaaactca tgttgtacgt
      781 gcccccactc tcttgtgatt ggattacggg gttttttcgt ggggaccata gacgagggag
      841 aagagtccgg attttccgcg gatttcgagc ttgcggtaga tattggtcag gtgttgtttg
      901 accgtctcgt ccgagataaa gagagcctca gcgatgtccg agcgattctt tccggccagg
      961 accaggttga caacctcttt ttcccgcttg gagatgggga gttgttcgac gcgatcggtg
     1021 agattctggt tcaggccgac ctgactctcg atccagccgc gttgttcatc catggccagg
     1081 ttgatgcttt ggtcccgcac tattgtgatc aggtgcggtt tcccctctat ctagacagac
     1141 cggtgagaga cctcgacggg gaggtccccg atcgaactac ggagcgtcat catccgggga
     1201 cgattctcca tattgtcaaa cacagcctcc ccgtcgtcgg gatggcttgt gaggatggac
     1261 tgcacctctt caccacaaag atcatttttc ttcctgtcaa gcatctggca gagtgtctgg
     1321 ttgcaatcaa tgatgatccg gttgcggctg acgattacgc catcgagcag gacatcggcg
     1381 atcatccgcc ggaattccgc ctcgcgccga tgtgtgtcga tgagtttttg ctcaagggcg
     1441 cgcagggaac cgaggcgcca ggcaacccag agcccgatca gatcgctgga aaccagggtg
     1501 acaatgatgg tgagggtggt cctgctttcg aggctgcgct ggacgaggag ggccgtcacg
     1561 tcgttggcga gaagggccag gaggggcatc gctcgtgaga gtatcccgcg ggtgggcacg
     1621 gcccaagcgg cgatgatgac gagtgtggtt atatagaagg aaacgctcga atcggggggg
     1681 cgagtccagg tgatcacccc actgatgagg gcagtcatgc tgatgatgag ttgcacaaga
     1741 ttcgagaaga gacgtgagcg tccactcttg atatagatga cacagcttgc cagaataaag
     1801 cctgagaaga ggatgcgaaa gacgataatg atgctgcaga gcgtgggatc actgatatcg
     1861 gtaagatcgt tacggatcat ggccagggag accagggaca taaagacaaa gatgccaatg
     1921 gtgatgctct ggaggtgacg gtcgttgggc atggttctct ctttgcgtac ggtgtcggac
     1981 tggtgttgtg tcgggaaaac gcacgagcag actgccggga accaggctca tgagcatctt
     2041 ccaatcgcat cctcaataat tatagcacat cgaacgagcg gggacgacag ggcctgcggg
     2101 cgacagacct gtaaaatttt ctgtcggagg agccgagtat tgcgtggcca gcgcatccgg
     2161 ttcttttttt gggtgaacag cctcaggccc gagatgggcg cccttgcggg gcgtgggatg
     2221 gccgaattcc ggcaagaatc ggttctgcga gatgtccccg ccaaagtatt acaaaattgt
     2281 catgcgactc cgggagtccc atcattgtat cgcaatatcc ctgcggggat ctccgcaggg
     2341 attgcacgga agatcgatca gacggggtga agcccggatt ggtgctactc cgccgctgat
     2401 tcccgtgtca gacggatgga gcctgtgaca aaaagcgcaa gtccgccgaa gtagggtgcc
     2461 aggaccagga cttcaaagag gggcccgtgg ggcaggaccg gagtaaatcg gccgatacca
     2521 atgaccgcat cggaggcgta gaggaggatg gcgccgaaac cggcggccag gccagccctg
     2581 ctgcggagca tgctgaatcc cgcggccagg aaggcag
//
LOCUS       DAOSHE010000302         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38026_length_2615_cov_2.730859, whole genome shotgun sequence.
ACCESSION   DAOSHE010000302 DAOSHE010000000
VERSION     DAOSHE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38026_length_2615_cov_2.730859"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLD82_08005"
     CDS             <1..885
                     /locus_tag="PLD82_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG65362.1"
                     /translation="RVWTSVTGETLSATIQRLRLEKSADLLLKYPDRSILEIALECGF
                     SGQAVFARSFKDAYGMSASAWRQGGAKVWVSDKGSTRRPLRKDCKTHGKECKGSSGGN
                     TYPHDVQATTGQSGQQPAETISWSDAMTAIKQAEEVRVSDDPAMTLAYVRHVGPYAGD
                     GELFGRLFGRLCAWAGPRGFLGPDATMLTIYHDNPEIVDDEKLRISVCCSVPEGTKPD
                     GDIGIMEMAAGRNVHARFELSTDEFGSAWNWLFSKWLPESGYQPDDRPCYEHYLNDPS
                     THPEGKHLVEIVTSIKPL"
     gene            complement(1116..2105)
                     /locus_tag="PLD82_08010"
     CDS             complement(1116..2105)
                     /locus_tag="PLD82_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65363.1"
                     /translation="MYAIVIFRDNCSSAPGVARYFPDGTDLPIEGTVSMCREVLGFAK
                     QFFRQTSCGWGCRVAKDISYPASKVIIPGSVFRKHQSTDNPGPLCPFGRADFVVVLDV
                     PAFAVLVIEQIQTVSEGFRIKGKARAVIEVIGSRYPTCFFGFTGRESHNEPVSQRTAL
                     LDPLADVQVRFHGGEVFDITVVLDREICSFADNSYPECGFALRMVEKVFLSQFSFSIV
                     GVDDPIIPVIVERTNFKFFISSIDISGQGMPAMLKSVPFGELACFPGFSSILVYGSEE
                     TCDRLCLGCGIKNNLCQKHAADKYKPCDFQLSVLLAMCRTAQDRRCMSGLRET"
BASE COUNT          674 a          743 c          649 g          549 t
ORIGIN      
        1 cgtgtctgga cttccgtcac gggagaaacg ctctcagcca caatccagcg cctccgcctc
       61 gaaaaatccg ccgacctgct cctgaaatat ccggatcggt caatcctcga gattgccctc
      121 gagtgcggct tctccggtca ggctgtcttt gcccgctcct tcaaggatgc ctacggcatg
      181 tcggcaagtg cctggcgcca gggtggcgcc aaggtatggg tcagcgacaa gggaagcacc
      241 cgccgtccac ttcgcaagga ttgcaaaacg catggcaagg aatgcaaagg atcatccggc
      301 ggaaacacgt atcctcatga tgtgcaggca acaaccggcc agagcggcca gcaacctgca
      361 gaaaccatct catggagtga tgcaatgaca gccatcaaac aagcggaaga agtacgagtt
      421 tcagacgatc cggccatgac cctggcctat gtccgccatg tcggcccata cgccggcgac
      481 ggagaacttt ttggcagact cttcggcagg ctctgcgcat gggcaggacc acgggggttt
      541 ttggggcccg acgcaaccat gctgacgatc taccacgaca atcccgaaat cgttgatgac
      601 gaaaagctcc ggatcagtgt ctgctgctcg gttcccgaag gaacaaagcc cgacggagat
      661 atcgggatca tggagatggc tgccggacgg aatgtgcatg cccgcttcga gctgtcaacc
      721 gacgaattcg ggagtgcctg gaactggctg ttttcgaaat ggctccccga aagcgggtac
      781 caacccgatg atcgcccctg ctacgagcac tatctcaacg atccgtccac gcaccccgag
      841 ggcaaacacc ttgtcgaaat cgtgacctcg atcaagccat tgtaagtatt ccaacagact
      901 atcccgacag accgggcaac aggcgaccaa tcagcggatc ggtcgcttgt tccggcttcg
      961 gtaaatctct tcaatgcaag tgcacacatg aacaatcgcg cgtggcgcta ccatcctccc
     1021 caataggatc cggattcaac aaattctgta agatttccga ctgaaacatg cctgtgatga
     1081 aatttgcaac tggtgtcaat caaccctgca gtgtatcatg tctcccgcag accggacatg
     1141 cacctccggt cctgcgctgt tctacacatc gcgaggagga cactcaattg aaaatcacaa
     1201 ggcttgtact tgtctgctgc gtgtttctga cacaagttgt tcttgatgcc gcaccccagg
     1261 cacaacctgt cacacgtctc ctcactgcca taaacgagta tggaggaaaa acctgggaaa
     1321 caagccagtt ccccaaaggg tacagatttc agcatggccg gcatgccctg cccagatata
     1381 tcaatactgc tgatgaaaaa tttgaaattt gtacgttcaa cgatcacagg tatgatcgga
     1441 tcatccacgc ctacgatgct gaaggaaaac tgcgacagga agactttttc caccatcctc
     1501 aaagcgaaac cacactccgg ataggaattg tcagcgaaac tgcatatttc cctgtctaag
     1561 acaacagtga tgtcaaatac ttccccccca tggaagcgta cttgtacgtc cgcaagagga
     1621 tcgagaaggg ccgtccgctg gctgaccggc tcgttgtgcg attctcgtcc agtgaagcca
     1681 aaaaaacagg tcgggtatcg ggaacctatc acttcgatta cagcacgcgc tttgcccttg
     1741 atccggaatc cctcactcac ggtttgtatc tgctctataa ccagtacggc gaaggctgga
     1801 acatcaagca ctacaacgaa atctgcacgc ccgaatgggc agagcggacc ggggttgtcc
     1861 gtcgactggt gtttccggaa gacggagcca ggaataatca ctttggaagc cggatacgat
     1921 atgtctttcg caacccggca cccccagccg caggacgttt gacggaaaaa ctgtttcgca
     1981 aacccgagca cttcccgaca catggagacc gttccttcta tcgggaggtc ggttccgtca
     2041 gggaaatacc gggcaacccc tggggcggac gaacaattat ctctgaatat cacgatggcg
     2101 tacaagacgc atcattcagg ggaatccgcc gaattgagtc agaacttgat gtacgcggta
     2161 tccgtaggcg agtggtaatc gatgggaacg gcattcatga caaatactgg ctgagcttca
     2221 gatcaaggga gaccagttgt tatcgtgtta taacactctt tgattcagcc gggtctgtcc
     2281 tgagcgaaga aacttttcac gattccaaaa aaaacagtct atacttcaat cgcacaatca
     2341 agcacttttc ccctgatggt tctgttcgtg gtaatacatt ctatcctgac tcgtcctgcg
     2401 gatctgaaga acgcctcctc tcatacacaa taaacaggta cggaatagtg accgcacgca
     2461 gctggagacc acctttcttc gagtatcaat cagcaggata cgacagactt gaaaactcgc
     2521 tctcaccagc cttgccggac cggatccagt ttgcagtcct tcccccctgg aaacaggcta
     2581 cggcaacgta cgaacaagca gggaccctga catta
//
LOCUS       DAOSHE010000303         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38092_length_2612_cov_4.157607, whole genome shotgun sequence.
ACCESSION   DAOSHE010000303 DAOSHE010000000
VERSION     DAOSHE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38092_length_2612_cov_4.157607"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLD82_08015"
     CDS             complement(<1..1454)
                     /locus_tag="PLD82_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65364.1"
                     /translation="MKWLSKAIVSDNDPFDKKELKIQLERHISIALCSPKKEIRQTGF
                     FYVLAWKRLDLIAGYGQGDGQALLRSALERLYEKHDFHYFSLKLLVRNDWAGSLASVA
                     ALLQKKIGEHEKKAYAAGLQSGDEILVARTLRTIDECVHVLGIPVVEFETLLVDFWRK
                     RQVVTGDLIAIGDIRRSRQYFAMVEPVRELLQAVGADHSRILSSYLDLAKGEDFFAGL
                     RWCLRWGCRDGMAKALDKIWPEHLGLVARLVRVHIESPALDQKRCEALVNLAGRNREG
                     VRELLELCMDPSALAPNLASAIEEGLCVSERRIDVFVLTGAMVEGMRVKSPEIISVRA
                     RFLSKIWDMVEGSEDVDLWDGALQILYELVDASRDTLIDGEFFLPLLREALFSESLTI
                     SRMANGYLKKRPDLKKVVLSWLSEPQLQTELASGLSSLRLQDQVKTLNRLVTLYCRYP
                     VPSAYLSKRVARYVEHLEKTRIEGGLKEKMRHAA"
     gene            complement(1736..>2612)
                     /locus_tag="PLD82_08020"
     CDS             complement(1736..>2612)
                     /locus_tag="PLD82_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG65365.1"
                     /translation="GIASITIAAGVETVFVLSNLQPGQAQDLWCILRGANGVLQTAPV
                     KLTVFTRSLATVSVPASSYQMGSANMDADMLPVHTVAISGFRAGTYEITWSQWTNVRT
                     WAATNGYDFGQSGSLGTGVSGRHPVVDLTWYDAIAWCNALSEMEGRTPVYYTTSAKVN
                     PYRDSRLTDLVYGSDWVRWDVNGYRLPTEAEWECMARYINGTVWHHGNLASGAGDGLH
                     GGYAVYGQNSGGVSAEVGTKLPNALGLYDMSGNVAEWCHDVYRSEGYDSSVQQDPKGP
                     APLEDGSSYRVFRGG"
BASE COUNT          593 a          829 c          592 g          598 t
ORIGIN      
        1 tcggccgcgt gtcgcatctt ttctttaaga ccaccttcga tacgggtctt ttcgaggtgc
       61 tcgacatacc tggcgacccg ttttgaaaga tacgcggatg gcacgggata gcgacaatat
      121 aatgtcacca gcctgttcag tgtcttcacc tgatcctgca gtctcagtga tgaaaggccg
      181 ctagccaatt ccgtttgcaa ctgcggttcg ctcaaccagc tcaagacgac tttcttcaga
      241 tccggtcttt ttttgagata tccgttcgcc attctgctga tcgtcaggct ctcggaaaag
      301 agcgcttccc gcaaaagtgg caaaaaaaac tcgccatcga tcagcgtatc cctggatgca
      361 tccaccagct catagaggat ctggagcgca ccatcccaga ggtccacatc ctccgatcct
      421 tcgaccatat cccatatctt tgacagaaat cttgcccgga cggaaataat ctccgggctt
      481 ttgacccgca tcccttcgac catcgcaccg gtcagcacaa agacatcaat tcgccgttcg
      541 gatacacaca agccctcttc aatcgcgctt gccagatttg gcgccaatgc agaaggatcc
      601 atgcacagtt ccaataattc cctgacaccc tcccggtttc tcccggcaag gttgaccagg
      661 gcttcacatc tcttctggtc aagtgcggga ctctcgatat gaactcggac caatcgtgcc
      721 acgagaccca agtgctcggg ccagatcttg tccaatgcct tcgccattcc gtcgcggcac
      781 ccccagcgca aacaccaacg gagtcccgca aaaaaatcct ctccttttgc caggtcaagg
      841 tagctggaaa gaattcggct atggtcggca ccaacggcct gcaacaactc tcgcaccggc
      901 tcaaccatcg caaaatactg ccggcttctc ctgatgtctc cgattgctat caggtcaccc
      961 gtcaccacct gccgctttct ccaaaaatca accagcaggg tttcaaattc aacgacaggg
     1021 attccgagaa catggacgca ttcgtcgatg gtccgaaggg ttcgcgcaac caggatctcg
     1081 tctcccgact ggagccccgc ggcataggcc ttcttttcgt gctcgccgat ttttttttgc
     1141 agcagggccg caaccgatgc aagactcccg gcccagtcat tcctcaccag gagtttgagt
     1201 gaaaaatagt ggaaatcatg tttttcatag agtctctcga gagccgaccg caaaagggcc
     1261 tggccatcac cctgcccgta tccagcaatc agatcgagac gtttccatgc aaggacataa
     1321 aagaacccgg tctgacgtat ttcttttttt ggagaacaca gtgcgatcga gatatgacgc
     1381 tcgagctgga tcttgagctc ttttttatcg aaagggtcat tatcagaaac aatggccttt
     1441 gacagccatt tcattccgtt ctcccagcag accggatctg cacagtccgc aacccatccg
     1501 tccgccgcta catcaatgct ctgcgctatc tcttcatcca ttgtggcaag agtataccag
     1561 acaccgcggg ggcagtcaaa ttttcttggt cattactcgc cagaccggct tagttcccgt
     1621 cccgaaccac aactctgaac ccgcgatccg cgtcatatcc agcaggatca tcggcgttcc
     1681 tgaacgagca ggtcatgacg ttgaaatcgg tcacaggatc ggtcaccgtc ctccatcagc
     1741 ctccgcgaaa aactctgtag ctgctcccgt cctcgagtgg tgccgggcct ttggggtcct
     1801 gctggacaga cgaatcatag ccctcggatc tgtagacatc atggcaccat tcggcaacgt
     1861 tccccgacat atcgtacaac cccaaggcat tcggaagttt tgtcccgacc tcggccgaga
     1921 cacctccgga gttttgcccg tacaccgcgt atccgccatg caatccgtca ccggccccgc
     1981 tggccaggtt tccatgatgc cacacagttc cgtttatgta tcgagccatg cactcccact
     2041 cggcttctgt tggcaaacgg tatccattga catcccaccg tacccagtcc gagccataga
     2101 ccagatccgt cagccggctg tcccgatagg gattcacctt ggccgaagtt gtgtaataca
     2161 ccggagtccg gccctccatc tctgagagtg cattgcacca tgcaatcgca tcataccatg
     2221 tcagatccac cactggatgt cgcccactga ctccggttcc caatgagcca ctctgtccga
     2281 aatcatagcc gttcgtcgct gcccacgtcc ggacattggt ccactgtgac catgtaatct
     2341 cgtatgtacc cgcccggaat ccggagatcg caacggtatg cacgggcaac atgtctgcat
     2401 ccatattggc cgatcccatt tggtaactgg atgccggcac actcactgtc gccaggcttc
     2461 tcgtaaacac agtcaacttg acgggagcgg tctgcaatac gccattcgca ccccgcagaa
     2521 tgcaccacag atcctgtgcc tgtccaggct ggagattgct caaaacaaac acggtctcca
     2581 cccctgccgc gatcgtgatc gatgcaatcc cg
//
LOCUS       DAOSHE010000304         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38170_length_2609_cov_3.409945, whole genome shotgun sequence.
ACCESSION   DAOSHE010000304 DAOSHE010000000
VERSION     DAOSHE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38170_length_2609_cov_3.409945"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1597)
                     /locus_tag="PLD82_08025"
     CDS             complement(35..1597)
                     /locus_tag="PLD82_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65366.1"
                     /translation="MEPDRALQGQALTPETFLQMLDCKAADLVLLCRHILKLQNVESV
                     LQRPLLGRLFIEASKIEEVLDAYGAKNSKHWCRFRSLVAALKTFSNATYVFLHIRNAA
                     PRYRLLPIEGDFFSDTGRCTTRLGAMLVSIVRTFVSEARRTGMAVRGRFRQASPLYDM
                     PLTGILPADRQLQHIASPAKVVVYLATAFLSESGQSLLQDLAGKLDRCRYAECVPDMF
                     SERSLRVAEREFHNLQAMYDTHISDTDAEQQDRNLPVIRGHVSIIYHLLEIATLLSHH
                     YERHIAGHGSGRSRTRPIVNVEELLEILIEYSLAYAGKFMKSAQHLCRAMIRRYAEPA
                     RIEVPVPVYRGFHVRPSTLVAKIVLHYGSEVYLETTDGSRYDASSPLELFRVNEKINA
                     EKRRMLAQHMAALPEIKQAAEESSAKQELMRRIFFRLLQKKDIVIYEGHLPFDDIKPE
                     DGEILPEFLKRALAYFLAHGLIDIRIDMQVAFCGDRRVLDDIRVLAEHGYGEDRYGND
                     VMLPPPLAYLKR"
     gene            1789..2241
                     /locus_tag="PLD82_08030"
     CDS             1789..2241
                     /locus_tag="PLD82_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG65367.1"
                     /translation="MMLQKYVSRERIVFFDAETHKQQALHRLMECITGSDLIADHDKF
                     RNGILERENIMSTGIGLGIAVPHTYSDAVRDIVIAIGVSREGISDYGSMDDKPVHFAL
                     MVAAGSHQHREYLQILARIAMLAKNERVKTALLAAKNAEEIISALESM"
     gene            2281..>2609
                     /locus_tag="PLD82_08035"
     CDS             2281..>2609
                     /locus_tag="PLD82_08035"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOG65368.1"
                     /translation="MAISYKSLGFVNTKNMFAKAVSGGFAVPAYNFNNMEQLQAIVLA
                     CLESQSPVILQVSKGARDYADQTLLTYLGQGAIEMMRRKAKEMGVGEIPIALHLDHGD
                     SFETCVS"
BASE COUNT          584 a          687 c          729 g          609 t
ORIGIN      
        1 tcaagcacat gcggtttgaa taattgccga atcttcagcg tttgagatag gccaagggcg
       61 gcgggagcat gacgtcgttg ccgtagcgat cttctccata tccgtgttcg gccagcacac
      121 ggatgtcgtc cagcacccgg cggtcgccgc aaaaggccac ctgcatgtcg atcctgatat
      181 cgatcagtcc gtgggccaaa aaatatgcca gagcccgttt gagaaattcc gggaggatct
      241 cgccatcctc gggcttgatg tcgtcaaagg ggagatggcc ttcgtagatg acaatgtctt
      301 ttttttgcag gagcctgaaa aagatcctcc gcatcagttc ctgtttggca gaggattctt
      361 cggcggcctg tttgatctcc ggcagggcag ccatgtgctg ggccagcatc cggcgttttt
      421 cggcattgat cttttcgttg acacgaaaca gttccagcgg ggatgaagca tcatagcggg
      481 atccatccgt ggtctcgaga tagacctcac tcccgtagtg gagaacaatt ttggcaacca
      541 gggtcgaggg cctgacatgg aatccccggt acacaggaac cggcacctcg atccgggccg
      601 gttcggcata gcgtctgatc atggccctgc agaggtgctg tgccgatttc atgaactttc
      661 cggcataggc cagggagtat tcgatcagga tctccagaag ttcttcgaca ttgacaatcg
      721 gccgggtccg ggatctgccg ctcccgtgcc cggcaatatg ccgttcataa tgatgcgaga
      781 gcaaggttgc aatctcgagc aggtggtaga tgatggagac atgtccacgg atgaccggga
      841 gattccggtc ctgctgctcg gcgtccgtgt ccgagatgtg ggtatcatac atggcctgaa
      901 gattgtggaa ctcccgttcg gcaacgcgca gagagcgttc gctgaacatg tccggcacac
      961 actccgcata ccggcaacga tccagcttcc cggccagatc ttgcagaagt gattgacccg
     1021 attcggacag aaaggccgtg gcaagataga caacgacttt ggccggcgag gcgatatgtt
     1081 ggagctgtct gtctgccggg agaatccctg tcagaggcat atcatataaa ggactcgcct
     1141 gtcgaaaccg cccgcgtacg gccattccgg ttcgacgggc ttcggagaca aaggtccgta
     1201 cgatggaaac gagcatcgct ccaagcctgg tggtacaacg tcccgtgtca ctgaaaaaat
     1261 cgccctcgat cggaagcagt ctgtagcgcg gagcggcatt cctgatatgg agaaacacat
     1321 aggttgcgtt tgaaaaggtt ttgagtgccg cgaccagact tctgaatcgg caccagtgtt
     1381 tgctgttttt ggcaccatag gcgtccagga cctcttcgat cttggaggct tcgataaaaa
     1441 ggcgtccgag aagcggtcgt tgcaagacag attcgacatt ctgcagtttg aggatatgcc
     1501 ggcagagaag aaccagatct gcagctttgc agtcgagcat ctgcaggaat gtttcgggag
     1561 tcaatgcctg tccctgaagc gcccggtccg gctccatacc taccctctgt gtcccgaatt
     1621 tctttgatgc tcaccagtct atcccggctc tttgggcatt gccagagaat tttcagctct
     1681 ggaaagacgg tacatcggtg gttggggaac cacggacccg aaagccgttt cttttttttt
     1741 cctgtctttt tgtgtattgt ttctttctca gaaccactcc cgggaggaat gatgctccaa
     1801 aaatatgttt cccgtgaacg aatcgttttt tttgatgccg agacacacaa gcagcaggcg
     1861 ctccatcgtc tgatggagtg cattaccggg tctgatttga ttgcggatca cgacaagttc
     1921 cggaacggaa tcctcgaacg tgaaaacatc atgagcaccg gcatcgggct gggtatcgcc
     1981 gtgccacata cctacagtga tgccgtccgg gatattgtca ttgcgatagg ggtttcccgc
     2041 gagggaatca gtgactacgg ctcgatggac gacaagccgg tccattttgc cctgatggtc
     2101 gcggcgggaa gccaccagca tcgtgaatac ctgcagattc ttgccaggat tgccatgctt
     2161 gccaaaaacg agcgggtcaa aaccgctctc cttgcagcca aaaacgcgga ggagatcatc
     2221 tccgcactcg agtcaatgta agcaacagca caatcttttg tatccataaa ggagcgtatc
     2281 atggccatca gctacaaatc actgggcttc gtcaacacaa agaacatgtt tgccaaggca
     2341 gtgtcgggcg gctttgccgt gccggcgtac aacttcaaca acatggaaca gcttcaggcg
     2401 atcgtcctcg cttgccttga gtcacaatct cccgtgatcc tccaggtttc caagggcgcc
     2461 cgcgactatg ccgatcagac cttgttgacc tatcttggtc agggcgcgat cgagatgatg
     2521 cgccgcaagg caaaggagat gggtgtcggc gagatcccga ttgcgttgca tctcgatcac
     2581 ggagattctt ttgaaacctg tgtttcctg
//
LOCUS       DAOSHE010000305         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38334_length_2601_cov_3.581304, whole genome shotgun sequence.
ACCESSION   DAOSHE010000305 DAOSHE010000000
VERSION     DAOSHE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38334_length_2601_cov_3.581304"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="fliW"
                     /locus_tag="PLD82_08040"
     CDS             <1..412
                     /gene="fliW"
                     /locus_tag="PLD82_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOG65369.1"
                     /translation="SEKQCISFPDGILGFEFIKEYYLLDIDNSPFYSLQASGEGEIAF
                     ILIRPQTFVREYRLEIGTADMEALGVRSVDELLDFAIVTVPENPAQMTANLQGPIVIN
                     KKTRIARQTISLNEDYCTKHPILAALQEKNREGG"
     gene            412..675
                     /gene="csrA"
                     /locus_tag="PLD82_08045"
     CDS             412..675
                     /gene="csrA"
                     /locus_tag="PLD82_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOG65370.1"
                     /translation="MLVLSRKTNQSIMIGDGIEIRIVDIKGDQVKIGINAPKQVAVYR
                     KEVYDDIKSQNIAAAQAGSLDLSGFPVLGSKKSGLGLDDPDGK"
     gene            857..>1938
                     /locus_tag="PLD82_08050"
     CDS             857..>1938
                     /locus_tag="PLD82_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG65371.1"
                     /translation="MLTRSGRFSIMLVVFTALFCFGLYSTARSILLQPISEDFGVSYT
                     GSGLMFLVSFLSVLVTNLAAGRLMQRYGMRVVYVASIAGISLVLLVQSLSADYLLFLL
                     STFVLTLFTSGQNLAGNSIVATAFAGDGGSRLNLMHVFYGLGSLAAPLFTGFLGADRL
                     EGVHQTIPAAIADVLFPVSGLDWRGIFAVLALLFGLVLFWYPFYRFRMQEAATIAPGR
                     AGFRHALGDRLLVKITVAVLFAAGYEVAVSSWLVYTLEQGAGVSPGGAGLAIAVFFAG
                     LAAGRASGSWLARTGRLNTILFPLAIAQAVLALVSLVAMDWMPGLFALAGLTSSIFFP
                     TLFYKVAQECHASASGAVYLTGAGLGV"
     assembly_gap    1939..1948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2082..>2601
                     /locus_tag="PLD82_08055"
     CDS             2082..>2601
                     /locus_tag="PLD82_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG65372.1"
                     /translation="MMRIRVVGNNAFAEELAGAWAEMHDCEWSAEMRPGRDLRIVQIL
                     CPPECDPRMLMDDLRAVLARELSQEEMPRLALRPVRRDWELAWQRFFRGRMVAERFWI
                     GPPWEETEIPDGLIPLVIDAGPAFGTGHHPTTELVLRLMTELDLAGARCLDAGTGSGV
                     LAIAACRLGAEEV"
BASE COUNT          443 a          752 c          783 g          613 t
ORIGIN      
        1 ttcagaaaaa cagtgcattt cctttccaga cgggattctc ggattcgagt tcatcaagga
       61 gtactacctg ctcgatatcg acaacagtcc cttctactct ttgcaggcat cgggtgaggg
      121 cgagatcgct tttatcctga tccggcccca gaccttcgtc cgtgagtacc gcctcgagat
      181 cggtaccgcc gacatggagg ccctgggtgt ccgctcggtg gacgagcttc ttgattttgc
      241 catcgtgaca gtccccgaaa atccggcaca gatgacagcc aacctccagg gcccgatcgt
      301 gatcaataaa aagacgcgga tcgcccgaca gaccatcagt ctcaatgaag actactgcac
      361 gaagcacccc attcttgcag ccctgcagga gaagaacagg gagggcggct gatgctcgtt
      421 ctctcacgca agaccaatca gtcgatcatg atcggtgacg ggatcgagat ccggattgtc
      481 gacatcaagg gcgaccaggt caagatcggt atcaatgcac cgaagcaggt ggctgtctac
      541 cgcaaagaag tctatgacga catcaaatcc cagaacatcg ccgctgccca ggccggcagt
      601 cttgatctga gcggtttccc ggtgctcggg agcaagaaat ccggactggg tctggatgat
      661 ccggacggaa agtaattccg gagcgcatca ggacccggca tatcctgaat ctgctcccgg
      721 gatctcgctg atcctccagt gatgcggtca gcgggatcaa ggatcggggt gttcctgaac
      781 acaaacggcc cggggtactg gcttttcttc ccgggaaagc ctatttttga atgatatact
      841 ccagtggtgc actgacatgc tgacccgatc cggccgtttc tcgatcatgc tcgttgtttt
      901 taccgccctc ttttgttttg gactctacag tacggcgcgt tccatcctgt tgcaaccgat
      961 ctccgaggat ttcggagtca gctatacggg cagcggcctg atgtttctcg tttccttcct
     1021 cagtgttctc gtgacaaacc tcgctgccgg ccgtctgatg cagcgctacg ggatgcgggt
     1081 cgtctatgtg gcgagtattg ccgggatctc cctggttctc cttgtccagt ccctctccgc
     1141 cgactatctg ctctttctcc tgagcacctt tgtcctgaca ctctttacct ccggtcagaa
     1201 cctggcgggc aactcgattg tcgcgactgc ttttgccggt gatggcggct caagactgaa
     1261 tctgatgcat gttttttatg gcctgggttc gttggcggct cccctcttta cgggatttct
     1321 cggcgctgac aggcttgagg gtgttcatca gacaatacct gcagcaatcg ctgacgttct
     1381 ctttcctgta tccggcctgg actggcgggg gatctttgcc gtgcttgccc tcctcttcgg
     1441 actcgtgctt ttctggtatc cattttaccg tttcaggatg caggaagcag caacaatcgc
     1501 acccggtcgc gccggcttcc ggcatgccct cggggaccgg ctccttgtca agatcaccgt
     1561 ggcggtcctc tttgccgccg ggtacgaggt cgcggtctcg agctggctgg tctataccct
     1621 cgagcagggc gccggtgtca gcccgggcgg tgccggtctg gcgatcgcgg tcttctttgc
     1681 cggtctggcg gcaggacggg cgagcgggag ctggctggcc cggacgggaa gactcaatac
     1741 gatacttttc ccattagcga ttgcccaggc tgtcctggcg ctggtttcgc ttgtggccat
     1801 ggactggatg ccgggactct ttgccctggc cggactgacc tcgtcgattt ttttcccgac
     1861 cctgttttac aaggttgccc aggagtgtca cgcttcggca tccggcgccg tctatctgac
     1921 tggagccggt ctcggtgtnn nnnnnnnncg tttcttgtgg gtgtggccgg tgacctgatc
     1981 acgatccgct gggccttggc tgtggctgtt ctctgtgcac tcctgcttgt tcccgcaata
     2041 cggtccgtga gcgtggaaga agcaaagagg aggctcacgc catgatgcgc atccgggttg
     2101 tgggaaacaa tgcctttgcc gaggaactcg ccggggcctg ggcggagatg catgactgcg
     2161 agtggtccgc cgagatgcga cccggccggg acctccggat agtgcagatt ctctgtccgc
     2221 ccgagtgtga tccccggatg ctcatggacg atttgagggc tgtcctcgca cgggaactct
     2281 cacaggagga gatgccccgt ctcgctttga gaccggtccg ccgggactgg gaactggcct
     2341 ggcaacgctt ctttcggggg cggatggtgg cagagcgatt ctggatcggc ccaccctggg
     2401 aggagaccga aattcccgat ggactgatcc ccctggtcat cgatgcgggt ccggccttcg
     2461 ggaccgggca tcatccgacg accgagcttg tgctccgctt gatgacggag ctggatctgg
     2521 ccggggcccg ctgtctcgat gcggggacgg ggagcggagt cctcgcgatc gcggcctgtc
     2581 gtctgggtgc ggaggaggtg a
//
LOCUS       DAOSHE010000306         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38358_length_2600_cov_4.047151, whole genome shotgun sequence.
ACCESSION   DAOSHE010000306 DAOSHE010000000
VERSION     DAOSHE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38358_length_2600_cov_4.047151"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLD82_08060"
     CDS             <1..1588
                     /locus_tag="PLD82_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HOG65373.1"
                     /translation="SGCGEDEEETAPLQGTSEASVSSTASSAVSLPGGATAFDETVRR
                     LAALDQPEALSEPVRIGQAVTNSEGETYTVVEHFRAAPEYSEVLALDPSSDVIWPGAL
                     LKGESVLAGDCVPVSGQRAPLVISISLENIQGNRSATVENPRLSAVRQALGEILAQNL
                     TGATPARVSFSIETVHDANQLELAIGASIGAGGNQIKAQFDFGDSRMRSRVLVRFVQV
                     YYTVDMDLPDRPSQIFAANADLPMIAGQLGNVAPLYVSTISYGRMAFFSFESEADGET
                     LKAAVSASCRGAVADGGASVSLADKTILSNARINATIIGGNGATAVNAVRGFEGLKLH
                     LLAGGNYDKNSPGAPVMYKLRYLSDNTIGRMVLLSEYEVRNVYRLFDWYRVYGMGLTC
                     DAEDDPGSEAEFFGDCSVTLTTNGAGVQGYENGAPRPAKIWNVMLMDAEDWALTPGAS
                     VNFPGEIRFRVSRFDLANSVMRVSARLKEQDSPGYDDMGEQESLLDLRNLPGGTAWLP
                     AFVDEGTRARIFFKVERVAE"
     gene            1613..2458
                     /locus_tag="PLD82_08065"
     CDS             1613..2458
                     /locus_tag="PLD82_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65374.1"
                     /translation="MKYYGMLCIGCCAVVLGLLAACSDDDETAKEGIDASSSIESGGT
                     GSSLEEAISSGGGMPSVSSLAAGSRSSGASLPPAGASTSSASAGGISSQGGTVSQSGG
                     ASQPAVSSSSLSSTTASSQSSSSSSQSTGGLVTLRVFSGRIVCDTEDDAGAEAEFYGT
                     CEVGAVCQGVSLQAMDGGYPVSGYLWEVESIRAEEWALQPGQSKSFSRQRSYRLDADE
                     LAACTLTIKAHVLENDTFGSDDLGWRELAVSGSAIPAGVAWLPAFSDGGTTARIGVMI
                     ELLTE"
BASE COUNT          499 a          687 c          868 g          546 t
ORIGIN      
        1 ctccggctgt ggggaggatg aggaggagac ggcgccacta cagggtacat cagaggcgag
       61 tgtctcatcg accgcttcgt ccgctgtcag tctgcccgga ggagcaacgg cattcgacga
      121 gacggtgcgc cggctggctg ctctcgatca gcctgaggct ctctccgagc cggtccggat
      181 cggacaggct gtcaccaata gcgagggtga aacctatacc gtggtcgaac acttccgggc
      241 ggcgccggag tacagtgagg ttttggcgct cgatccttcg tcggatgtga tctggccggg
      301 cgccttgctc aaaggggaga gtgttctggc tggggattgt gttccggtct ccggacagcg
      361 ggccccgctg gttatttcca tttcactgga aaatatccag gggaaccgat cggccacagt
      421 ggaaaatccg cggctttccg ctgtccggca ggcgctcggc gagattctgg cacaaaatct
      481 gacaggggcg acaccggcca gagtcagctt ctcgatcgag acggtgcatg atgccaatca
      541 gctcgaactg gcgatcggtg cgagtatcgg tgcgggtgga aaccagatca aggcccagtt
      601 cgattttggt gacagcagga tgcgttcccg ggttctggtg cgctttgtcc aagtctatta
      661 taccgttgac atggatcttc cggatcggcc ctcgcagatt tttgctgcca atgccgatct
      721 gcccatgatt gccggccagc tcgggaatgt ggcgcctctc tatgtctcca ccatcagtta
      781 tgggcggatg gcgtttttct ccttcgagtc cgaagcggac ggggagaccc tcaaggcggc
      841 tgtctcggca tcctgccggg gggccgtggc tgatggcggg gcgagtgtct ccctggccga
      901 caagaccatt ttaagcaatg ccaggatcaa tgcgaccatc atcgggggaa acggggctac
      961 ggccgtcaat gcagtcaggg ggtttgaagg gctcaagctc caccttctgg cgggcggcaa
     1021 ttatgacaaa aactcgcccg gggcaccggt gatgtacaag ctgcgctatc tctcggacaa
     1081 cacgatcgga aggatggtgc tcctgagcga gtacgaggtg cggaatgtct accggctctt
     1141 cgattggtat cgcgtatacg ggatgggttt gacctgcgat gcggaagatg atcccgggag
     1201 cgaggccgag tttttcggtg actgtagtgt gaccctgaca accaacgggg caggagtcca
     1261 ggggtacgag aacggggctc cccgtccagc gaagatctgg aacgtgatgc tgatggatgc
     1321 cgaggactgg gcgctcaccc cgggagcatc cgtcaatttt ccgggcgaga tacggttcag
     1381 ggtctcccgc ttcgatttgg ccaattccgt gatgcgtgtt tcggcccggc tcaaggagca
     1441 ggactcgccg ggatatgacg acatgggcga acaggaatcg ctgcttgatc tgcgcaacct
     1501 tccgggaggg acggcctggc ttccggcctt tgtcgacgag ggtacccggg cgcgcatctt
     1561 tttcaaagtc gaacgggttg cagagtaagc agaaaccgga aaggagtaca gaatgaaata
     1621 ttatggaatg ctttgtatag gctgctgtgc tgtcgtgctg gggcttctgg ccgcgtgcag
     1681 cgatgacgac gagaccgcaa aggagggaat tgatgcaagc tcatccattg aatcaggagg
     1741 aacaggctca agcctcgagg aagccatcag ttccggcggg ggaatgccgt cggtgtcatc
     1801 cctggcagcg gggtcacgat cctcgggggc gagtctgccc ccggccggcg cgagtacttc
     1861 atccgcgtcc gcgggcggga tatcttccca gggtggaacg gtctcacagt cggggggggc
     1921 gagccagccg gcagtgagtt cctcgtctct ttcttcaacg acggcatctt cccagtcctc
     1981 gtcttcatct tcgcaatcca cgggcgggct cgttaccctg cgcgtttttt caggccggat
     2041 tgtctgcgat accgaggacg atgccggggc ggaagccgaa ttctacggga catgcgaggt
     2101 tggtgcggtc tgtcaagggg tcagcctgca agccatggat ggtggatacc cggtctcagg
     2161 gtatctctgg gaggttgaat ccatcagggc cgaggagtgg gctttgcagc ccgggcaatc
     2221 aaaaagtttc agccggcagc gttcgtaccg gctggatgcc gacgagcttg ccgcgtgtac
     2281 cctcaccatc aaggcgcacg tcctggagaa cgacaccttt ggttcggatg atcttggctg
     2341 gcgggagctg gcagtttcgg gcagtgccat tccggccggg gtggcctggt tgcccgcttt
     2401 ctccgacgga gggacgacgg ccaggatcgg ggtgatgatc gaattgctga cggaatagca
     2461 gaagcagggg ggggacagaa aaaggccccg tctggggcct ttttctgatg ccggcacgat
     2521 gtttcttaaa gcttgtaatt gataccgcca aggattgtca acatgttgac agaactgctg
     2581 gcaaggatca tgtgaaagcg
//
LOCUS       DAOSHE010000307         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38472_length_2595_cov_3.192913, whole genome shotgun sequence.
ACCESSION   DAOSHE010000307 DAOSHE010000000
VERSION     DAOSHE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38472_length_2595_cov_3.192913"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1466)
                     /locus_tag="PLD82_08070"
     CDS             complement(192..1466)
                     /locus_tag="PLD82_08070"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF006964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HOG65375.1"
                     /translation="MIRLTKFGDKLCRESALYEMMDDLGAGSDAGEQRVALMGGGNPA
                     AIPGIEVVWRTRMQEIMNDGERFERMLGAYDSPQGNREFLEILAAYFNRECGWNLTYR
                     NIVITNGSQNAFFFLFNMLAGECPDGKKRRVLFPLCPEYIGYADQGLEPDLFTARLPL
                     VRETGPHAFRYEPDFAGLNIGDDIGLVCLSRPTNPTGLVLSRAEVARLDRMAAARGIP
                     LLVDAAYGAPFPGIVFADGGIDWSENLILTWSLSKIGLPAVRTGIVVASEEMIHKLAT
                     VNAVISLATSGIGQTLVAPLFADGRITRMADETVRPFYRKKRDEFVRELERAFGAAGV
                     DWRLHECEGGFFVWLWFPGLREPATAVYRAAKARGVIVTPGEAFFTGPDQAELAAWPH
                     QRQCLRLNYADTEGDIPRGLAILAEEIARRQG"
     gene            complement(1708..>2595)
                     /locus_tag="PLD82_08075"
     CDS             complement(1708..>2595)
                     /locus_tag="PLD82_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG65376.1"
                     /translation="FEALHAPGGVLMYGIPEFRLPKALVQREIDSIRKLGVEIVCNVI
                     VGRSITIDELFEEEGFAAVFVGSGAGLPSFLRIEGENLNGVYSANEFLTRSNLMKAYE
                     FPKSPTPIRVGRRVAVVGGGNVAMDAARTAKRLGAEEVYIVYRRGEDELPARAEEVHH
                     AKEEGIIFKLLNNPVKVHGENGWVTGLECMEMTLGEPDASGRRRPVAKEGSNFVIGLD
                     TVVVAIGQSPNPLIRQTTPGLDTERWGGIIVNEETMKTSKDGVYAGGDVVTGAATVIL
                     AMGAGKTAARAIHEALEKR"
BASE COUNT          517 a          908 c          698 g          472 t
ORIGIN      
        1 cttccggcga tgcacacatt gatatcacgt ttttcttatg cgtatcccgg atacccataa
       61 cattttcgca atatccttat acgtatctcc ccacacacac aacggggact tcgcggattc
      121 acataacaaa tccatgtttt tgtggtctga atcccggatt cgcatgtcat acccataatt
      181 ttgtgaccgg ctcacccttg ccgccgggcg atctcttcgg ccaggatcgc cagtccccgg
      241 gggatatccc cctcggtgtc cgcgtagttc aggcgcaggc actgccgctg atgcggccag
      301 gcggcaagct ctgcctgatc cggcccggta aaaaacgcct cgcccggtgt gacgatcacg
      361 ccccgcgcct ttgccgcccg atagaccgcc gtcgccggtt cacgcagacc cgggaaccag
      421 agccagacaa aaaatccgcc ctcacactcg tgcaaccgcc agtcaacccc ggctgccccg
      481 aatgcccgct ccagctcgcg cacaaattcg tcacgctttt tccggtaaaa cgggcgcacg
      541 gtttcatccg ccatccgcgt aatccgcccg tcggcaaaga gcggcgccac cagggtctgc
      601 ccgatcccgc tcgtcgccag actgatgacc gcattgaccg tggccagctt gtggatcatc
      661 tcttcggacg cgaccacgat cccggtccgc accgccggca gcccgatctt ggagaggctc
      721 caggtcagga tcaggttctc gctccagtcg atgccgccat cggcaaagac gatccccgga
      781 aaaggcgccc cataggcggc atcgaccaaa agcgggatgc cgcgggctgc cgccatccgg
      841 tcaagccgcg ccacctcggc ccgtgacagc accagcccgg tcgggttggt cggccgggag
      901 aggcagacca acccgatatc gtcgccaata ttcaagcccg caaaatccgg ctcgtagcga
      961 aaggcgtgcg gcccggtctc gcgcaccagg ggcaggcggg ccgtaaagag atcgggctcg
     1021 agcccctgat ccgcataccc gatatactca ggacagagcg gaaagaggac acgccgcttt
     1081 ttcccgtccg gacactcgcc tgccagcatg ttgaacaaaa agaaaaacgc gttctggctg
     1141 ccattggtaa tcacgatatt tcgatatgtg agattccagc cgcactcacg attaaaatag
     1201 gcggccaaga tttcgaggaa ctcccggttt ccctgggggg aatcgtaggc gcccagcatg
     1261 cgttcaaagc gctcgccgtc attcatgatc tcctgcatcc gcgtccgcca gactacctcg
     1321 atcccgggga tggccgccgg gttcccgccc cccatcagcg ccacccgctg ctcgcccgca
     1381 tcactccccg cgccaaggtc atccatcatc tcgtagagcg cactctcgcg acagagcttg
     1441 tcaccaaatt ttgtcagcct gatcatgacg gcatccccct tgcacgcatc cggccgcaaa
     1501 ccgctatcgg ccactcctca ccgggcgaca caagcccccg gacccagtat agacccaagc
     1561 gcccgcttcc ggccagaggc tgttccccaa aaagaaacag acccgcaccc acgcacacct
     1621 cggcaacatc gtgcattcac gagcataacc ccggaatgaa aaaagccgcc cttggcggag
     1681 cggctttgcg gcagcgcacg ggcttgttca gcgcttttcg agggcctcgt ggatggcgcg
     1741 ggcggcggtc ttgccggcac ccatggccag gatcacggtc gcggccccgg tcacgacatc
     1801 accaccggcg tagaccccgt ccttggaggt cttcatcgtc tcctcgttga caatgatccc
     1861 gccccagcgt tccgtgtcaa gtccgggtgt cgtctgacga atgagcgggt tgggcgactg
     1921 cccaatggcc acaaccacgg tatccaatcc aatgacgaaa ttgctgccct ctttggcaac
     1981 cggccggcgg cgtccggagg catccggctc gccaagggtc atctccatgc actccagccc
     2041 ggtcacccag ccgttctcgc cgtgcacctt gaccggattg ttgaggagct tgaaaatgat
     2101 cccctcctct ttggcgtgat gcacttcctc ggcacgggcc ggaagttcgt cctcgccgcg
     2161 gcggtagacg atgtagacct cttcggcccc gagacgcttg gccgtcctgg ccgcatccat
     2221 ggccacattg cctcccccga caacagccac ccggcgtccg acccggatgg gcgtcgggct
     2281 cttgggaaac tcataggcct tcatcaggtt ggagcgtgtc aaaaattcgt tggccgaata
     2341 aaccccgttg agattttcgc cctcgatccg caggaaggac ggcaggccgg ccccgctccc
     2401 cacaaagacg gccgcgaaac cctcttcctc gaagagctcg tcaatcgtga tcgagcgccc
     2461 gacgatgacg ttacagacga tctcgacgcc gagcttgcgg atggaatcga tctcgcgctg
     2521 caccagcgcc ttgggcagac ggaactcggg tattccgtac atcaatactc cacccggggc
     2581 atgcagggcc tcaaa
//
LOCUS       DAOSHE010000308         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38572_length_2590_cov_3.085207, whole genome shotgun sequence.
ACCESSION   DAOSHE010000308 DAOSHE010000000
VERSION     DAOSHE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38572_length_2590_cov_3.085207"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="PLD82_08080"
     CDS             complement(<1..1828)
                     /locus_tag="PLD82_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG65377.1"
                     /translation="MNGADADSIKEKASAGSIHWIHILLALALPFVFVTGMHPAFIPV
                     KTAVLVFGAAALSLAMVFWIRGRTETVLNIRREHLYLAGFVVWMLASIFWSPDLFGAL
                     AEWLPLASAAMIWFSWSLYYGNEDQDNRKQFELVQTVVLVGLLESLVVLVQYFGFGLR
                     FNEDVVGKWRVVGTFGNPNYVAEFLVPVFFLALNLLHHETRTVHRKFYLASIFFLVLA
                     IAATWSRTGWLLLAGGLVLYAWIFTRQSRQRISSMLYLVMARARWYLLSLFALLTVVL
                     VLFPVIRIDRTLMRHAANTASISGRFFIWEHSLDAMVDHVPFGSGIGGFAKAWEDAQY
                     RHFSTGDTSGVNHAWINDRAHNDFIQYAVEGGIGVILFILFWARILRKGAGGFRHNDL
                     SIGVFVALVLLLVSSLGNFPFRVPPNLWLVMFLAALVSQHRHLDNEQTIFRLDLSRAK
                     NRMLGPLLVFALVGVVWGTGHILSAAHLRAGEDALGKKDEENAREHFRLAVHFDPGNA
                     RAHIRYARLLADYGDLRGADRHARQSLDILPFPEAWAVSALAAASSGSNELAVSLLER
                     RSTTYRKIPGFAADTIRALADAGKVDAAFLEVQRLNAILGEID"
     gene            complement(1825..2130)
                     /locus_tag="PLD82_08085"
     CDS             complement(1825..2130)
                     /locus_tag="PLD82_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65378.1"
                     /translation="MWCRACSKEVSIAGRVPFRERCPHCDAALHSCVNCRFYDSAAYN
                     SCREPKADRQVEKQRENRCEYFETMTVPPGMTAKKPQTSKDAFEALFAKQDKSEEQE"
     gene            complement(2130..>2590)
                     /locus_tag="PLD82_08090"
     CDS             complement(2130..>2590)
                     /locus_tag="PLD82_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG65379.1"
                     /translation="PLAGGVFAPVFNKLFLAERGQGAVCNQQKIRVSSVDTLEEALFV
                     TGFPYEREGRIPMLQRMIGNALGHVQGIRRMGSAALDLCHVASGLFDVYIEFEIKPWD
                     MAAGVLVLEEAGGKVSRCDGSPLLLDGRQIVASNGILHDPALRNILDGVL"
BASE COUNT          660 a          738 c          709 g          483 t
ORIGIN      
        1 gatcaatctc gccaaggatc gcattcaacc gctgaacctc gagaaaagcg gcatccactt
       61 ttccggcatc agccagggcg cggatggtgt cggctgcaaa accgggaatc ttccggtatg
      121 tcgtactccg ccgttcgagg agagagaccg ccagttcgtt cgaaccggat gatgcggccg
      181 cgagtgcgga gactgcccag gcttcgggaa acggcaggat atcgagtgac tgtctggcat
      241 gtctgtcagc gccgcgtaga tccccgtaat cggcaagaag tcgggcatac cggatatggg
      301 ctcgggcgtt ccccggatcg aaatggaccg ccaatctgaa gtgttcccgg gcgttttctt
      361 cgtctttttt cccgagcgcg tcttccccgg cacgaagatg ggcggccgag aggatgtgtc
      421 ccgttcccca gacaacgccc accagtgcga aaaccagcaa cggtcccagc atccggtttt
      481 tggcgcggga gagatccaga cggaaaatgg tctgctcatt gtccaggtgc ctgtgttgac
      541 tgaccagcgc agcaagaaac atgaccaacc agagattggg tggaaccctg aacggaaaat
      601 ttccaaggga agaaaccaga agcaaaacca gcgcaacaaa cacaccgatg gaaagatcat
      661 tgtgccggaa gccgcccgcg cccttgcgca ggattctcgc ccagaaaaga atgaacagaa
      721 tgacgccgat ccctccctcg accgcgtatt ggatgaaatc gttgtgggcc ctgtcgttga
      781 tccatgcatg gttgacgcca gaggtatccc cggtcgagaa gtggcggtat tgggcatctt
      841 cccatgcttt ggcaaatccg ccgatgccgg aaccgaacgg aacgtgatcg accatggcat
      901 ccagggagtg ctcccagatg aaaaagcgtc ccgagatgga agccgtattc gccgcatgcc
      961 gcatcaaggt cctgtcgatc ctgatgaccg gaaagagaac caggacaacg gtcagaaggg
     1021 caaagaggga aaggagatac cagcgagccc gggccatgac gagatagagc atgctcgaga
     1081 tccgttgacg gctctgtcgc gtaaaaatcc aggcgtagag caccaggcct ccggccagca
     1141 agagccagcc tgtccgcgac caggttgccg caatggccag gacaaggaaa aaaatcgaag
     1201 cgagatagaa tttccggtga acagtcctcg tttcatggtg gagaaggttc aatgcgagaa
     1261 aaaagactgg caccaaaaac tccgcaacat aattcgggtt gccaaacgtc cccacaacac
     1321 gccattttcc gacaacatcc tcgttgaaac gcagaccgaa accaaaatac tggacgagaa
     1381 caaccagtga ttcgagaagg cccacgagaa caaccgtctg gacgagttca aactgtttgc
     1441 ggttgtcctg atcctcgttc ccgtaataca gactccagga aaaccagatc atggcggccg
     1501 aagccagcgg aagccattcg gccaaagccc cgaagaggtc cggcgaccag aaaatggatg
     1561 ccagcatcca gacaacaaaa ccggcgagat agaggtgttc ccgccggata ttcaatacag
     1621 tttcggttcg cccacggatc cagaagacca tggccagaga caaggccgct gcaccaaaca
     1681 caaggacagc ggttttgacc gggatgaacg cgggatgcat cccggtcaca aatacaaaag
     1741 gaagtgcgag agcaagcagt atatggatcc agtgaattga tccggctgaa gctttttcct
     1801 tgattgaatc cgcatctgct ccgttcattc ttgctcctca ctcttgtcct gttttgcgaa
     1861 aagtgcttca aaagcatcct tggacgtctg cggttttttg gccgtcattc ccggtggaac
     1921 agtcatcgtt tcaaaatact cgcagcggtt ctcgcgctgt ttttcaacct gccgatcggc
     1981 ctttggttcc cgacaactgt tgtaggccgc gctatcataa aaccggcagt tgacacacga
     2041 atgcagggcg gcatcacaat ggggacagcg ttccctgaac gggaccctcc cggcaatgct
     2101 cacctctttg gaacatgccc gacaccacat cagagtactc cgtccaggat attgcgaaga
     2161 gccggatcat gcagaatccc gttgctggcg acgatctggc ggccgtccag aagaagcgga
     2221 cttccatcac aacggctcac ttttccgcca gcctcttcca gaaccaggac accggccgcc
     2281 atatcccagg gcttgatctc gaattcgatg taaacatcaa aaagaccgct ggccacatgg
     2341 cagagatcca gggcggccga ccccatcctg cggattccct gaacatgacc aagggcattc
     2401 ccgatcatcc gttgcagcat gggaatccgg ccctcccgtt cgtaggggaa cccggtcacg
     2461 aagagggctt cttcgagggt atcgaccgac gagacccgga ttttttgctg gttgcagacc
     2521 gcaccctgtc cccgctcagc gagaaagagt ttattgaaga caggagcgaa gacaccgccc
     2581 gcaagcggga
//
LOCUS       DAOSHE010000309         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38760_length_2581_cov_2.932304, whole genome shotgun sequence.
ACCESSION   DAOSHE010000309 DAOSHE010000000
VERSION     DAOSHE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38760_length_2581_cov_2.932304"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..976
                     /locus_tag="PLD82_08095"
     CDS             32..976
                     /locus_tag="PLD82_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65380.1"
                     /translation="MIVSRRTSILLVLLAAALTACGGDAWKDLTKVGNTWEYEGETTK
                     AKVTAKVLGTWSIGETVYAEIAPDFGRYTNVTELCSWNKDSKTFLHAGESSRFNDKTR
                     QFFVKETVIGAVEGKANTAFTNSDATVLRYLGEETIKVPAGEYTAYKFSRINTRRKIN
                     EIWWYADKVGIVRVKNSKDTFGLVKYTEGKEQDPKAMGATVEGVAFVKKFFAAAKKGE
                     MQGVKSLFAGGAGQQDFASQAPEITLLVDRIKSGVRDDQATWSYLGAKTYGLRFLKYD
                     ESGAEPNVWKVDAVMELTNQGGQLMIKKIGIANNKLIN"
     gene            1333..1659
                     /locus_tag="PLD82_08100"
     CDS             1333..1659
                     /locus_tag="PLD82_08100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG65381.1"
                     /translation="MGGAERRRETRYTIKQMVAVSYAHEEFIAAHGLDLSSGGISCRT
                     TSPLDVGQEIFLLLGLGKDETISCEGVVLHVSPDGSGFKAGIQFVNLSSEDSARLAAH
                     LGTLKG"
     gene            complement(1813..>2581)
                     /locus_tag="PLD82_08105"
     CDS             complement(1813..>2581)
                     /locus_tag="PLD82_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOG65382.1"
                     /translation="WWEEMTDEPPASLTDWNGNPWTADNGKKAAHPNARFTAPAAQCP
                     VIDSAWQDPRGVPISAILFGGRRPSVVPLVHESFDWNHGTFLGSIMASEMTAAAAGTV
                     GKLRRDPFAMLPFCGYHMGDYFAHWLSMGTKSTPDKLPKIYYVNWFRKGSDGKFLWPG
                     YGENSRVLKWIIERVAGTAEAITTPIGNIPTRDGLDLSGLDLPAGALDELLKVDIAEW
                     KNEIPGIREHFATFGEHLPQELAAQVDALEKRLQRQG"
BASE COUNT          562 a          692 c          798 g          529 t
ORIGIN      
        1 ctaccgcgtg tccaacgagg gaggcatgac aatgattgta tcgcggcgga catcgatcct
       61 gctcgtactg cttgcagcag ctttgacggc gtgtggtggc gacgcatgga aggatctgac
      121 gaaggtcggc aatacctggg aatacgaagg cgagacaacc aaggccaagg tgacagccaa
      181 ggtgctggga acatggtcca tcggggagac cgtctacgcc gagatcgctc ccgattttgg
      241 caggtacacc aatgtgaccg agctgtgcag ttggaacaag gacagcaaga ccttcctgca
      301 tgcgggtgaa agctcccggt tcaacgacaa gacccgccag ttctttgtca aagagacggt
      361 tattggagcg gttgaaggca aggccaatac ggcatttacc aacagtgacg ctacggtcct
      421 gcgctatctt ggtgaagaaa caatcaaggt gccggccgga gaatacacgg cctataaatt
      481 cagccggatc aacacccggc gcaagatcaa cgagatctgg tggtacgctg acaaggtggg
      541 catcgtccgg gtcaaaaaca gcaaggatac cttcggtctt gtcaagtata ccgagggcaa
      601 ggagcaggac cccaaggcga tgggcgccac ggtcgaggga gttgcctttg tcaaaaaatt
      661 ctttgcagct gccaaaaagg gtgagatgca gggcgtcaag tcgctctttg ccggcggcgc
      721 tggtcagcag gattttgcca gccaggcccc ggagatcacc ctgcttgtcg acaggatcaa
      781 gagcggtgtc cgcgacgatc aggcgacctg gtcctatctc ggggccaaaa cctacggcct
      841 gcggttcctc aagtatgacg agtcaggggc agagccaaac gtctggaagg tggacgccgt
      901 gatggagctg actaaccagg gcgggcagtt gatgatcaag aagatcggga ttgccaacaa
      961 caagttgatc aactgacagc aagcacagtg ctgatgcgac cgtcgggctt tggtccggcg
     1021 gttttttttt atatccatgc acgccacagg cgtctctgat tgagatgccg gccgcgaagg
     1081 tccccttgga cgggagtgca tcagtccgta taatgattct gtaatgtgac tccggagttc
     1141 catcacaaga atagaatatc tcttgtgccg aacgagtctg cggatgtgct tctgtctctc
     1201 tgcgttgttt atgataatcc gggacgattt tttgcgtgaa cgcatggccg gcatggctca
     1261 accatgaaaa atctgtgcat tcaggcgatg atatgctata ctggtccgaa caaatcaggg
     1321 ttggagacgc gcatgggcgg agcagagcgg cggcgtgaaa cgcgctatac gatcaagcaa
     1381 atggtggccg tgtcctatgc ccacgaggag ttcatcgcgg cgcacgggct ggatctctca
     1441 agtggcggga tctcctgccg caccaccagc ccgctcgatg tcggacaaga aatatttttg
     1501 ctgctcgggc tgggtaagga tgagaccatc agttgtgaag gtgttgtcct gcacgtcagc
     1561 cccgatggca gtgggttcaa agccgggatc cagttcgtca atctctcgtc cgaggacagt
     1621 gcccggctgg cggcccatct ggggacactc aaaggctgag ccatgtccgg agaaagacgt
     1681 caagagaaga gtcatttttc ttgatgaaca acctcaggcg ggggatgcgt cagcctttcg
     1741 caaagggttc tcatgaaggc gctgtttgaa aaaaagccgg ggcggaatgg acccgacacc
     1801 cggcgctcac aatcaacctt gcctctggag acgcttttcc aatgcatcca cctgggcggc
     1861 gagttcctgc gggaggtgct cgccaaaggt ggcgaagtgc tcgcggattc ccgggatctc
     1921 gtttttccat tcggcgatat caaccttgag gagttcgtca agggcccccg ccggaaggtc
     1981 caatccgctc agatccagcc catccctggt cgggatgttt ccgatcgggg tcgtgatcgc
     2041 ctcggccgtt cccgcaaccc gttcgatgat ccacttgagg acccggctgt tttcaccgta
     2101 gccgggccag aggaacttcc cgtcagatcc cttgcggaac cagttgacgt aataaatctt
     2161 cggcaatttg tccggggtgc tctttgttcc catggaaagc cagtgggcaa aatagtcccc
     2221 catgtggtat ccgcagaagg ggagcatggc aaagggatca cggcgcaatt tccccacggt
     2281 cccggcggca gcggccgtca tctcgctggc catgatcgag cccaaaaagg ttccgtggtt
     2341 ccagtcgaag gactcgtgga ccaggggcac gacggagggc ctgcgcccgc cgaagaggat
     2401 ggccgagatc gggacgcccc tggggtcctg ccaggcgctg tcgatcaccg ggcactgggc
     2461 cgcgggtgcg gtaaagcggg cgttgggatg tgcggccttt tttccgttgt ccgctgtcca
     2521 gggattgccg ttccagtccg tcaggctggc gggcggctcg tcagtcatct cttcccacca
     2581 g
//
LOCUS       DAOSHE010000310         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_38853_length_2576_cov_4.380801, whole genome shotgun sequence.
ACCESSION   DAOSHE010000310 DAOSHE010000000
VERSION     DAOSHE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38853_length_2576_cov_4.380801"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLD82_08110"
     CDS             <1..611
                     /locus_tag="PLD82_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOG65383.1"
                     /translation="WLFLEIPVRLNFAVIPGLPNSHSFAKRARAAGHQVLIHLPMQPR
                     AGEKTETGSGVVQAGMSSRDIERILDRAVRSVPGAVAVNNHMGSLATADRSLMRLLMP
                     AIRQRGLLFLDSKTHASSVAASEADRAGLPILVRDIFLDHVQKRTYIEDALRSLVREA
                     AGKGFAIGIGHVTCMETWQVLRDELKKIAEIGVDFVGITELE"
     gene            611..1714
                     /gene="tsaD"
                     /locus_tag="PLD82_08115"
     CDS             611..1714
                     /gene="tsaD"
                     /locus_tag="PLD82_08115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG65384.1"
                     /translation="MLILGIETSCDDTAVAVVRDGHEILSNCISSQDEFHKAFSGVVP
                     EIASRRHLEVIHLLIDKALRDSGVSPGQIDRIAVSNRPGLIGSLLVGVAAAKACALAW
                     RKPLVPVNHLAAHLYAATFTEPMPYPHIGLVISGGHTLLVEAQSPLDVIIHGSTIDDA
                     VGEVYDKIAKHFGLGYPGGPVLDRLAAGGDPGSWNFPRSLLDPVTQRYHFSFSGIKSA
                     VIHQRERFASHPPEAVHQKTDHEEVKNTSGALAAHERIEDIAAAFQAAVADILITKTA
                     RLVVDLGLQTVAVTGGVAANGYLRQRFADEAGFHAVFPPRPLCTDNAVMVAGAAWHDS
                     VPLEGEAILRLDALSRVVLRGKRRQTVSGSSLS"
     gene            1711..>2576
                     /locus_tag="PLD82_08120"
     CDS             1711..>2576
                     /locus_tag="PLD82_08120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG65385.1"
                     /translation="MTEDGRRRPILAILLCMAFFPFVLSAAGNSGTHWESQDGYLPLS
                     TLVDDMGVEVHWHPVLGKLDLVRAGGRLTLLTGSPLVTSASGRVDRLSSPLKRTSGAI
                     LVPLDTMRHLYRELSLPGEIWSLTVGKTPTAIRRLTNTAARPDPGQTAERPGGQGIDL
                     VVLDAGHGGQDPGAVGYRGAHESVLVLDITRRVERRLKAAGIRVFLTRKGNETVSLER
                     RADMANDLLSKGNKGIFVSIHANASLSTARNGFETYFLSPIASSADARSTAAIENGAL
                     VADLPSEGKGAM"
BASE COUNT          459 a          780 c          804 g          533 t
ORIGIN      
        1 aatggctctt tctggaaatc ccggtcaggc tcaattttgc cgtcataccc ggcttgccga
       61 attcccacag ttttgcaaaa cgggcccggg ctgccggcca tcaggttctg atccacctcc
      121 cgatgcagcc ccgtgccggg gagaaaacgg agaccgggtc cggggtcgtg caggccggga
      181 tgtcctcccg tgacatcgag cgaatcctgg atcgggcggt ccggtccgtt cccggggccg
      241 ttgccgtcaa caaccatatg ggctccctgg ccaccgcgga tcgaagtctg atgcgacttc
      301 tcatgccggc tatccgtcaa cggggactgc tcttcctgga cagcaaaacc catgcgagct
      361 cggtcgccgc ctcggaagcc gaccgtgccg ggctccccat tcttgtcagg gacatttttc
      421 tcgaccacgt ccagaaacga acctatattg aagatgccct tcgctccctg gtccgggagg
      481 ccgccggaaa gggttttgcc atcggtatcg gccatgttac ctgcatggaa acctggcagg
      541 ttctgaggga cgaactcaag aagattgcgg agatcggtgt ggatttcgtc gggataacgg
      601 agcttgagtg atgctgattc tcggtattga gacatcctgt gatgatacgg cggtggcagt
      661 cgtccgggac gggcacgaga tcctttccaa ttgcatttcc tcccaggacg agtttcacaa
      721 ggcgttttcc ggagtcgtgc ccgagatcgc ctcgcggcga catcttgaag tcatccacct
      781 tctgatcgac aaggccttgc gtgattcggg ggtgagtccc gggcagatcg accggatcgc
      841 cgtcagcaac aggccaggac tgatcggatc cctcctggtc ggggttgccg ctgccaaggc
      901 ctgcgccctg gcctggcgca aacccctggt ccccgtcaac catcttgcgg cccatctcta
      961 tgccgcgacc tttaccgagc cgatgcccta tccccatatc ggactggtca tctccggggg
     1021 gcataccctc ctggtcgagg cacagtctcc tctggatgtg atcattcacg gatcgacgat
     1081 cgacgatgct gtcggggagg tctatgacaa gattgccaag catttcggcc tcggctatcc
     1141 gggtgggcct gtccttgacc ggctggctgc cggaggggat cctgggtcct ggaacttccc
     1201 gcgctccctt ctcgatcctg tgacccagcg ctatcatttc tcgttttccg gaatcaagag
     1261 tgcggtcatc catcaacggg aacgcttcgc gagtcacccg cctgaggctg ttcatcaaaa
     1321 aacagaccat gaagaagtga aaaatacctc cggggcgctt gcggcccacg agaggattga
     1381 agatatcgcg gcagcattcc aggccgcggt ggccgacatc ctcatcacca agaccgcccg
     1441 gcttgtggtc gatcttggct tgcagactgt ggccgtgacc ggcggggtgg cggccaatgg
     1501 ctatctgcgc cagcgttttg cggacgaggc ggggtttcac gcagtctttc ctccgagacc
     1561 gctctgcact gacaatgcgg tcatggtggc gggggcggcc tggcatgatt ccgtcccgct
     1621 tgagggagag gcgatcctcc ggctggatgc actctcccgg gttgtcctgc ggggaaaacg
     1681 ccggcagacg gtctccggga gcagtctctc gtgacggagg acgggcggag acgacccatt
     1741 ctggccatcc tgctctgtat ggcttttttc ccgtttgtcc tttctgctgc cgggaactcc
     1801 ggaacccatt gggagagcca ggacggatac cttccactgt cgacgctggt ggatgacatg
     1861 ggggtcgaag tgcactggca tccggttttg ggaaaactcg acctggtcag ggcagggggg
     1921 cggctgaccc tgttgaccgg atccccgctt gtaacgagtg cctcgggtcg ggtggaccgg
     1981 ctctcctctc ccctcaaacg gacatcgggt gccatccttg ttcccctcga tactatgcgg
     2041 cacctctatc gggaactctc ccttccgggc gagatctggt ccttgacggt cgggaagacc
     2101 ccgacggcca tccgtcgctt gacaaacaca gctgcccggc ccgatccagg gcagactgcg
     2161 gagcgtccgg gagggcaggg cattgacctg gttgtcctgg atgccgggca tggggggcag
     2221 gatccggggg ccgtcggcta ccggggggcg catgaatcgg tcctggtact ggatattacc
     2281 cggagggtcg agcgccggtt gaaagccgcc ggaatccggg tgtttttgac ccgcaaggga
     2341 aatgaaacag tcagcctcga gcgcagggcg gatatggcca atgatctcct ctcgaaaggg
     2401 aataaaggga tctttgtcag catccacgcc aacgcatccc tctcgacagc ccggaacgga
     2461 ttcgagacgt atttcctgtc cccgatcgcg tccagtgcgg atgcccggtc cacggcggcc
     2521 atcgaaaacg gtgccctggt cgcggacctt ccgagcgagg ggaagggtgc catgga
//
LOCUS       DAOSHE010000311         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39108_length_2565_cov_3.367331, whole genome shotgun sequence.
ACCESSION   DAOSHE010000311 DAOSHE010000000
VERSION     DAOSHE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39108_length_2565_cov_3.367331"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLD82_08125"
     CDS             complement(<1..1123)
                     /locus_tag="PLD82_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOG65386.1"
                     /translation="MNQVLHETDPQQGLSSQEARARAKAGLGNDWEPEPTASLLWIFV
                     SNIFIVFNLMIFPLLAVLLALGQYKDVISVGGIALLNTLINILQEVRAKLALDRIRLE
                     HPALIRVIRDGREQEIPSRDVVSGEILVLSAGERIVADGPVVRAEHVELDESMLTGES
                     EYVGKEPGQIVLTGSFVIAGRGLYRAEKLGQDAWIQTVVRDSRKHTDRRTELEKRVDL
                     LVRVCMILCFLVAGLLLLSHDPFASGLVDPVRFIVASVTSLIPQGLVLIVTVAFALGV
                     IAFARRGIIFRRMNAVEGLAGIDLICMDKTGTLTHNDITLDSIVSLESAGPRLDEHGV
                     RELLGAFAWSVTARNKTVEAIQRALPDDGGWTRLAEIPFK"
     gene            complement(1120..1311)
                     /locus_tag="PLD82_08130"
     CDS             complement(1120..1311)
                     /locus_tag="PLD82_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG65387.1"
                     /translation="MSISVRGERCPQDHPCPAIRFCPVRAISQDGFKAPVVDQAVCID
                     CGRCMQVCPHGVFVSEQPV"
     gene            complement(1346..2149)
                     /locus_tag="PLD82_08135"
     CDS             complement(1346..2149)
                     /locus_tag="PLD82_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG65388.1"
                     /translation="MRVTILVDNLAERGGLLAEHGFALHIETSAKRILFDTGASGTAL
                     VHNAQRLGIDLSLVDELVLSHGHSDHTGGLGAFLALNSRASIRMSPAGWVPKYKGREA
                     YIGLPLAREAYEERVVPVEQPLWIAADVRVMTAAPILDQTDTHFEGMYVKEGQLFKQD
                     EFQDELSLLIENAEYYGVVSACSHRGITNILEEATRLAGEAPAVVIAGMHLRQSSPEQ
                     IDPIVEGLASRGLVQFHSAHCSGPLATAMLRERMPGRVEWTSAGKVIEF"
     gene            complement(2305..>2565)
                     /locus_tag="PLD82_08140"
     CDS             complement(2305..>2565)
                     /locus_tag="PLD82_08140"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOG65389.1"
                     /translation="EIHAIINAVDGCHGAHEIRTRMIGSTLAVDMHAQVDGGMTVRDG
                     HAIATRIEKVLRKKYGRDTIISVHIEPVTDRGRRRHAARYKE"
BASE COUNT          539 a          818 c          705 g          503 t
ORIGIN      
        1 atttgaacgg aatctccgcc agacgagtcc agcctccgtc atccggcaat gcccgctgga
       61 tggcctcaac agttttattc cgtgccgtca cactccaggc aaaggcccca aggagttcac
      121 gcacaccgtg ttcatcaagc ctcggaccgg cggactcaag ggaaacgatg ctgtccaatg
      181 tgatatcatt atgtgtcagt gtcccggtct tgtccatgca gatcaggtcg atcccggcca
      241 gtccttccac ggcattcatc cgccggaaga tgattccccg gcgggcaaag gcgatgacac
      301 ccagggcaaa ggcaaccgtc acgatcaaga ccaggccctg gggaatcagg gaggtcacgg
      361 aggccacaat gaaccggacc ggatccacca gcccgctcgc aaagggatca tggctcagga
      421 ggaggagtcc ggcaacgaga aagcagagga tcatacagac ccgaacgagc agatccacac
      481 gcttttcgag ctctgtcctc cggtcggtgt gtttccggga atcccggacg actgtctgga
      541 tccacgcgtc ctgtcccagt ttttccgccc tgtagagccc ccggccggcg atgacaaaac
      601 tccccgtcaa tacaatctgg cccggttcct tcccgacgta ctccgactcc ccggtcagca
      661 tggactcatc cagttcgacg tgttccgccc ggaccaccgg cccgtcggcc acaatccgtt
      721 cgccggcgga gagaaccagg atctcgcccg acacaacatc ccgggacgga atttcctgct
      781 ccctgccgtc acggatcacc cggatcagcg ccggatgctc aaggcgtatc cggtccaggg
      841 ccagcttggc ccgaacctct tgaaggatgt tgatgagggt attcaaaagg gcgatccccc
      901 cgacggagat gacatccttg tactggccca gagccaaaag gacggcgagg aggggaaaaa
      961 tcatcaggtt gaaaacgatg aaaatgttgg atacaaagat ccagagaagg ctggccgtcg
     1021 gttccggctc ccagtcattc cccaaaccgg ccttggctcg tgcccgggcc tcctggctgc
     1081 tcaggccttg ctgcggatca gtctcgtgca ggacctgatt catacgggtt gttcgctgac
     1141 aaacacacca tgcgggcaga cctgcatgca gcgtccacaa tcgatacaga cggcctgatc
     1201 aacaacgggg gccttgaacc cgtcctggct gatggcccgc accggacaaa accggatggc
     1261 cgggcaggga tggtcctggg ggcaacgctc ccctctcacg gaaatcgaca tggttctctg
     1321 cttcctttcc tctgtgtacc aagactcaga actcgatcac cttgcccgca ctcgtccatt
     1381 cgacccggcc cggcatacgt tccctgagca tggccgttgc cagaggcccg ctgcaatgcg
     1441 cggaatggaa ctgcacaagt cccctggatg ccagtccttc gacgatcggg tcgatctgtt
     1501 cgggtgaaga ctgccggaga tgcatgccgg caatcacaac cgccggggct tctcccgcca
     1561 gacgggttgc ttcctcgaga atattggtga tcccccggtg cgagcaggcg gaaacaaccc
     1621 cgtagtactc ggcgttttca atcagcaggg agagttcatc ctgaaactcg tcctgcttga
     1681 acaattggcc ttccttgaca tacattcctt caaaatgggt atcggtctgg tcaagaatgg
     1741 gggctgccgt catcacccgg acatcggccg cgatccagag gggctgctcg accggtacca
     1801 ccctctcctc gtaagcctca cgcgccaacg gcaacccgat ataggcttcc cgccctttat
     1861 acttgggcac ccagccagca ggagacatcc ggatggatgc cctggaattg agggccaaaa
     1921 aggcgcccaa ccctccggta tggtcgctgt gcccatggct caggacaagc tcgtccacga
     1981 gggagagatc gatcccgagg cgctgggcat tgtggaccag ggccgtcccg ctggccccgg
     2041 tatcaaagag gatccgtttg gcggaggtct cgatatgcag ggcaaagcca tgctctgcca
     2101 gcagtccgcc cctctcggca agattgtcca caagaatcgt tactctcata cctgacccgc
     2161 cttccttttt gccagtttac ctgtcctctg cggttgtttc aagcatcatg acgtaccacg
     2221 tgccttggca acagatattc cgcagattcg acaagtgctg ttcatccgga aagacgggca
     2281 gccggtcaag cagcaaagac tggatcattc cttatagcgc gcggcatgcc ggcgccttcc
     2341 ccgatcagtg accggttcga tatggacgga gatgatcgta tcccgcccgt atttcttccg
     2401 gagcaccttt tcgatccggg tagcgatggc atgcccgtca cggacggtca tcccgccatc
     2461 cacctgggca tgcatgtcca ccgcaagcgt gcttccgatc atccgtgtcc ggatctcgtg
     2521 ggcgccatgg catccatcca ccgcattgat gatggcatgg atctc
//
LOCUS       DAOSHE010000312         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39237_length_2560_cov_4.066667, whole genome shotgun sequence.
ACCESSION   DAOSHE010000312 DAOSHE010000000
VERSION     DAOSHE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39237_length_2560_cov_4.066667"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLD82_08145"
     CDS             complement(<1..762)
                     /locus_tag="PLD82_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HOG65390.1"
                     /translation="MDEQSQSPCVVAEALIAAVLPSAAAWGIVPARRDDAARARYEDW
                     LAEGLHAGMPCLTDYAHVKFDPSRVLPAAASLILVALPYGGAGKDSAAGGHGADAAAE
                     TGEAGLVARVSRGRDYHKVLQTMLRRICAALQARFPDGHFRPVCDTVPLSERFYAARA
                     GLGTVVHSCLLGNMEMGTRFVLGAVVSSNEFMVRDAGLGLAENPCTDCGLCLEACPTR
                     ALVGPGRLDVSRCISWLTIEYKGLIARDLWPAIGRR"
     gene            complement(771..1889)
                     /locus_tag="PLD82_08150"
     CDS             complement(771..1889)
                     /locus_tag="PLD82_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65391.1"
                     /translation="MRLVSSIRAGLAAALLALCFPLVLPAQNAGVDSPTLTALSRYFA
                     GLEQPAGSPFTRQTQVGGYPWFKSEMNRYWNNFRTTTFQHIRSWQKTEATDLSRQTVL
                     YPFSGPDFLNVYAVYPEADTYIMIGLEKGGLVPDLEGMSVANIQKGLNMMVEGFRIYI
                     GFNFYRTLGMEVDLNLSPFTGTLPHVLTQIAWLGYTPLSVSSVSFTAQGQIVTKPLAI
                     GQVTTSWQLECRSSAGKTMKIIFLSQDVSNSGLAAVPGLKRYLESLPRVSGLFKAASY
                     LPPREFFTEMTKICLSKMNSIVQDDSAIPYRMLANDYDVTVYGFYDRPHGLFPSYGQP
                     DLAQLYRQRPRKALAFNFQYDRPDGSRNLMVARRKAGR"
     gene            2156..>2560
                     /locus_tag="PLD82_08155"
     CDS             2156..>2560
                     /locus_tag="PLD82_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG65392.1"
                     /translation="MCHDCPRRCGVDRAHGIEGWCRLPGYEPWIASICRHTGEEPALG
                     GTGGVANVFYTHCNLACVYCQNADISQRSLPLPDPVPVSEAVRRIEDLLARGARAVGF
                     VSPSHQPRATAAIIDALRARGVRAPMIWNSSAY"
BASE COUNT          529 a          766 c          807 g          458 t
ORIGIN      
        1 acgtcgcccg atggcgggcc agagatcacg cgcaatcaat cctttgtact caatggtcaa
       61 ccaggagatg cagcgcgaga catccagccg gcccgggccg acgagggccc gtgttgggca
      121 ggcctcaagg cacagcccgc aatccgtaca cggattttcc gccaggccga gcccggcatc
      181 acgaaccata aattcgttgg aggaaacaac ggccccgagg acaaagcgtg tccccatctc
      241 catgttcccc aagaggcagc tatgcacgac ggtccccaga ccggcccggg cagcgtagaa
      301 gcgttcggaa agtggaaccg tatcgcagac cggccgaaaa tggccgtccg gaaaccgtgc
      361 ctgcaaggct gcgcagatcc ggcgcaacat ggtctgcagc accttgtggt agtcgcgccc
      421 acgggagacc cgggccacca gcccggcctc ccctgtctct gctgctgcgt ccgctccgtg
      481 tcctcctgcg gcggaatctt tacctgcacc cccataaggc agggccacca ggatcaggct
      541 ggcggccgcg ggcaggaccc gggatggatc gaatttgaca tgtgcgtaat ccgtcaggca
      601 gggcatcccc gcgtgcaggc cttcggccag ccagtcttcg tagcgtgcgc gggccgcatc
      661 atcccgccgg gcagggacga tcccccaggc ggcggccgag ggtaacacgg ccgcgatcag
      721 ggcctccgcc acaacacagg gggactgaga ctgttcatcc aaaaaaagtc ttacctgcca
      781 gccttgcgcc tggccaccat gagattgcgt gagccatcgg ggcggtcgta ctggaagttg
      841 aaggccagtg ccttgcgcgg gcgctggcgg tacaactggg ccaggtcggg ctgtccatag
      901 gaggggaaga gcccgtgcgg ccggtcataa aaaccgtaca cggtcacgtc atagtcattg
      961 gccagcatgc ggtagggaat ggccgagtcg tcctggacga tgctgttcat tttcgaaaga
     1021 cagattttag tcatctcggt aaagaactcg cggggcggaa ggtaggatgc cgccttgaag
     1081 agacccgaga cccggggcag gctttcgaga tagcgcttga gtccgggaac cgcagccagg
     1141 ccgctattgg aaacatcctg actgaggaag atgatcttca tggtcttgcc tgctgaagag
     1201 cggcactcca actgccagga ggtcgtcacc tgtccaatag ccagaggctt ggtcacaatc
     1261 tgtccctgtg cggtaaagct gaccgatgaa accgacagcg gggtataccc aagccaggcg
     1321 atctgggtca ggacgtgcgg cagggttccg gtaaagggtg agaggttgag atcgacctcc
     1381 atccccagcg tgcggtagaa gttaaacccg atgtagatcc tgaagccttc gaccatcatg
     1441 ttgagccctt tttgaatatt ggccacggac atgccctcca gatcggggac gagcccgccc
     1501 ttttcgaggc cgatcatgat ataggtatcc gcctcgggat agaccgcata aacgttgagg
     1561 aagtcgggac ccgaaaacgg ataaagcacc gtctggcggg acagatcggt tgcctcggtt
     1621 ttttgccagc tgcggatgtg ctggaaggtt gtggtgcgga aattattcca gtagcggttc
     1681 atctcgctct tgaaccaggg gtacccgccg acctgggtct gccgggtaaa agggctgccg
     1741 gcgggttgct caagtccggc aaaatagcgc gaaagcgcgg tcagggtcgg gctgtcgacg
     1801 cctgcatttt gtgcgggaag gacaagggga aagcagaggg caagcagggc cgcggccagg
     1861 cctgcacgaa tggaagaaac gagtctcacg aaggaagctc cttgatggtt gaagtactgt
     1921 gtaccgccgg tacgctgtca ttagacacgc atttgtcaaa tatcgcactc cccgatctgc
     1981 ctggggaaaa gaaagcagcg cgaggccggc tgtgaaaccg ggagccaaat catggtatca
     2041 tgaacaagtg gtgcgaatcc gggagtcaca taccattttt gtaatactgc actggctatc
     2101 ccggattgct gccatcccct ccttttggcc gtacattttg tggatgcaaa gcgacatgtg
     2161 ccatgactgt ccacgccggt gcggggttga ccgggcccac ggaatcgagg gctggtgccg
     2221 cctccccggc tacgagccat ggatcgcctc gatctgccgg cacacgggcg aggagccggc
     2281 cctcggtggc acgggtggcg tggccaacgt gttttacacg cactgcaacc tggcctgtgt
     2341 ctactgccaa aatgccgaca tcagccagcg cagcctgccc ctgcccgatc ccgtcccggt
     2401 cagcgaagcc gtacggcgga tcgaggacct gctcgcacgc ggggcccggg ccgtcggctt
     2461 tgtctcgccc tcgcaccagc cgcgcgccac ggcggccatc attgacgcac tccgcgcccg
     2521 cggggtcagg gctcccatga tctggaacag ctcggcctat
//
LOCUS       DAOSHE010000313         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39270_length_2559_cov_2.992412, whole genome shotgun sequence.
ACCESSION   DAOSHE010000313 DAOSHE010000000
VERSION     DAOSHE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39270_length_2559_cov_2.992412"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="PLD82_08160"
     CDS             <1..1896
                     /locus_tag="PLD82_08160"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOG65393.1"
                     /translation="ARHCRIAICELKSLLELPAGTTYYFCDIHGQGDKFFHIINNKAG
                     TLKRKIDDILDDLTPDARIRLLKICYYPREELEWEKENCRREYTAPVEGYLFSIGRMI
                     RHVGSKTTRREFYQQIESSIFHAAILELVTASNDDKIIRNNSLTYQKSLIAEYREAGL
                     QEDLVCEMVRILKNLMIHKYVINGDIPDRGPDTARILEYLMTDPRVEINWGNHDLMWM
                     GAAAGSQELVAELIRVQLRYDNYDILEREYGIPLQPLAQFAESTYSSQPVKGFTPSVF
                     SGNGHYSHQLVAKMQKAVAVILWKLEAARNASLGVTPYLSLLCTNDSGNLGVTIGDTI
                     HELLDQDFPTLDLSHPSRLTREEKTVLDTLVSSFKKSTRLRKHMTMLAERGSLYHIQD
                     GILSFHATVPVNPDGSLRSIPVLGDMFSGKALFDRLNQLYKSAFATKRPKQDVLDLFY
                     LGWKGPHSWTFGKSGMETFTRALVADKTTHTEEKSPYYILLANPKTAEALSRTIMDDF
                     ERDGSPSKIEKIFNGHIPVKIEKNESAVRAGGRVICGDGGFSEAYGDIGFVLVTTSSG
                     IYLNKLGRSVTATEILANNADILPVILWEEKFPERKQLSACNIAPEILAKISALETIL
                     AELTGKA"
     gene            complement(1914..>2559)
                     /locus_tag="PLD82_08165"
     CDS             complement(1914..>2559)
                     /locus_tag="PLD82_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG65394.1"
                     /translation="AVRERVEGSGRTDLGEYSENIERMMDVVEVRTREMLARFRMPGR
                     WVDLDAAGIREGLLQVLDAIALNSHGRFGFVYDSGGSDSRYGFSLQIEGALAGQVRMP
                     PVFVDCLRDLVANARKYTQPGGTIEAILNQTGDGFSIKVKDTGLGIPADEIQQVVEFG
                     VRGSNVRDIPTRGGGFGLTKVWYATRQYGGRLWIASGQGQGTLVHVWVPRPVQT"
BASE COUNT          663 a          791 c          578 g          527 t
ORIGIN      
        1 gcccgacact gccgcatcgc catctgtgag ctcaaaagcc ttctggagct accggctggc
       61 acaacctatt acttttgtga tatccatggt cagggagaca aattcttcca cattatcaac
      121 aacaaagccg gaacactgaa acgaaaaatt gacgacatcc tcgatgatct gactccggat
      181 gcacgaatcc ggctcctcaa aatttgctat taccctcggg aagaattgga gtgggaaaaa
      241 gagaattgcc gccgggagta taccgcgcct gtcgaaggct atctcttttc aatcggacgc
      301 atgatccgtc acgtcggctc aaaaacaacc cgcagggagt tctatcagca aatcgaatcc
      361 agcatcttcc atgctgccat cctcgaactc gtgactgctt ccaatgatga caaaatcatc
      421 cggaacaact ctttgacgta ccaaaaatcc ctgattgccg agtatcgcga ggcagggctc
      481 caggaagacc tcgtctgcga aatggttcgc atcctgaaaa acctgatgat ccacaaatat
      541 gtcatcaacg gcgacattcc ggacagagga ccggacaccg cacggatcct cgaatatctc
      601 atgaccgacc ccagagttga aatcaactgg ggaaaccatg acttgatgtg gatgggcgcc
      661 gcagccggaa gccaggaact cgtggcagaa ctgatccgcg tccagctccg ctatgacaac
      721 tatgacattc ttgaacggga gtacggaatc cccctgcagc cgctggccca atttgccgaa
      781 tcaacctatt cctcccaacc ggtcaagggt tttactccgt ccgttttttc cggaaacgga
      841 cactatagtc accagctcgt cgcaaaaatg caaaaagccg ttgcagtcat actctggaaa
      901 ctcgaggctg cacgaaacgc cagcttgggc gtcactccat atctttcgct cctgtgcacc
      961 aacgattctg gcaacctggg ggtaacaatc ggtgacacaa tccacgagct tcttgaccag
     1021 gatttcccga cactggatct ctcccacccc tcgcgactga cccgagaaga aaaaaccgtc
     1081 ctggacacac tggtctcctc cttcaaaaaa agcacccgtc tccgaaaaca catgaccatg
     1141 ttggctgaac gtggttcact ctatcatatc caggacggaa tcctcagttt ccacgcaacc
     1201 gttcctgtca acccggacgg cagtctcagg tcaatcccgg tacttggcga tatgttctcc
     1261 ggaaaagccc tttttgaccg attgaaccaa ctctacaaat ccgcatttgc gacaaaacgc
     1321 ccaaaacagg acgtcctgga tcttttttac ctgggctgga agggcccgca ctcctggact
     1381 tttggaaaat ccgggatgga aacgtttacc agagccctgg ttgccgacaa gacaacgcac
     1441 acagaggaaa agtcgccgta ttacatcctc cttgccaacc caaagactgc cgaggccctt
     1501 tcccggacca tcatggatga tttcgaacgc gacggctcgc catcaaaaat tgaaaagatc
     1561 ttcaatggcc acattcccgt gaaaatcgaa aaaaacgaaa gtgccgtccg tgccggaggc
     1621 cgggtcatct gtggcgatgg aggattctcc gaagcctacg gggatatcgg cttcgtcctg
     1681 gtaacaacct caagcgggat ctatctcaac aagctcggac gttcggtcac cgccacggag
     1741 atcctggcga acaatgcaga catcctgcct gtcatcctct gggaagaaaa atttcccgaa
     1801 cgaaaacaac tttctgcctg caatatcgcc cctgaaatcc tggcaaaaat ctccgctctc
     1861 gaaactatcc tggcggaact gaccggaaaa gcatgacctg acacaccaca ccatcacgtt
     1921 tgcactggcc gaggcaccca tacatgaacc aaggtcccct gcccctgtcc ggaggcaatc
     1981 cagaggcgcc cgccatactg tctggtcgca taccatacct tggtcaagcc gaatccgcca
     2041 ccccgggtcg ggatatcgcg gacatttgaa ccacgcactc caaactcgac aacctgctga
     2101 atctcgtccg caggaatacc caggcctgta tccttgacct tgatggaaaa cccgtcccct
     2161 gtctggttga gaatcgcttc gatcgtcccg cccggttgtg tgtactttct cgcattggcg
     2221 acaagatccc tgagacaatc gacaaaaacc ggcggcatcc tgacctgacc ggcgagggct
     2281 ccttctatct gcagggaaaa gccgtaccgg gagtcacttc caccggaatc atagacaaac
     2341 ccgaatctgc catgggaatt gagcgcaata gcatcaagaa cctgcagcaa accttcccgg
     2401 ataccggcag catccagatc gacccatctc cccggcatcc tgaatctggc cagcatctct
     2461 ctggtccgga cctcgactac gtccatcatc cgctcgatgt tctcgctgta ttcgccaaga
     2521 tccgtccggc cactgccttc gacccgctcg cgaaccgca
//
LOCUS       DAOSHE010000314         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39339_length_2556_cov_2.481407, whole genome shotgun sequence.
ACCESSION   DAOSHE010000314 DAOSHE010000000
VERSION     DAOSHE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39339_length_2556_cov_2.481407"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLD82_08170"
     CDS             <1..370
                     /locus_tag="PLD82_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG65395.1"
                     /translation="PWPWPGRPDQVRLETALRPLLGRHDFTSYTLVDQSAKTRERTIH
                     SIGVEMHDRNLDLVFTADGFLRRMIRMITGQLVEAWAAEDPAAVIQSVLDARNNAFCA
                     APAPPDGLYLEEIRYPGDET"
     gene            428..1216
                     /gene="cdaA"
                     /locus_tag="PLD82_08175"
     CDS             428..1216
                     /gene="cdaA"
                     /locus_tag="PLD82_08175"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG65396.1"
                     /translation="MEFLKDIYFFREILLPAIDIFVVYYLVYRVYLIIAETRAIAALK
                     GIILIVAVTLAANFLGLETLGWLLERFLGVAAIALIVLFQPELRRVLMKVGQGGLLTM
                     YMDDRREYLKDIIEAVEFMSSRRLGALIVFERGVGLKNFVETGTQLEASVSFELLISV
                     FSKESPLHDGAVIIQHKRISAAGCYLPLTQRRDIRKEYGTRHRAAIGLSEETDAAILV
                     VSEETGEISLAVDGKIRSDIRQEKLAENLLKVLGERERARKKKK"
     gene            1213..2145
                     /locus_tag="PLD82_08180"
     CDS             1213..2145
                     /locus_tag="PLD82_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOG65397.1"
                     /translation="MSIRDLFLRNWLQKTISMTIAVALFVFVRSMLTHELRVPVQLRI
                     KNPPSYLMSVGKMEESITVTVRGIKGKIDSIEGSRITASVDMADAVPGLNEFPVILHY
                     PGYSEDIQLTTSVRSLRLQMDYAMERMIPLTPVIKGSPADGFAIHETNIQPAMLLVRG
                     PAMILSNLASLPTMPFSVSGLKSAISSPVQVDLGNMNLEIRQSGQIVLRLGILPERKT
                     RVFQALVPVVEGLGPGLKMVQPEELPKVRLILSGDAARIDQIKEPELRLVLSLTNLQS
                     GIHEVKITPVLPEGIRVRSAEPGSVAVELAEGIP"
     gene            2142..>2556
                     /locus_tag="PLD82_08185"
     CDS             2142..>2556
                     /locus_tag="PLD82_08185"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG65398.1"
                     /translation="MKSILGVNIDHVATVREARKGPMPEIVTAAGIAEQSGAHGITIH
                     LRQDRRHIQDRDVAILKQVVHSKLNLEMSITEEIVLIALATVPDQATLVPENRQEVTT
                     EGGLDVAGQIGRVREVTQRLASAGTLVSHFIDPELR"
BASE COUNT          538 a          707 c          747 g          564 t
ORIGIN      
        1 accctggccg tggccggggc gcccggatca ggttcgcctc gaaacagcgc tccggccctt
       61 gttgggacgg cacgacttta cgagctatac ccttgtggac cagtcggcca aaacacggga
      121 acgcaccatc cactccatcg gagtcgagat gcacgaccgg aacctggatc tggtctttac
      181 cgcagacgga tttttgcgga ggatgatccg catgatcacc ggacaacttg tcgaggcatg
      241 ggcggcagag gatccggcag ctgtgatcca atccgtcctg gatgcccgga ataacgcgtt
      301 ctgtgccgct ccggcaccgc cggacggcct ctatctcgaa gagatccgct atcccgggga
      361 tgaaacatga ccggcgacgg tccggctgag tatatttagt gttcatgacg ggagaaagac
      421 ggcggcaatg gaattcctca aggatatcta ttttttcagg gagattctcc tgccggccat
      481 cgacatcttt gtcgtctact accttgtcta tcgtgtctat ctgatcattg ccgagacccg
      541 tgccattgcc gccctcaagg ggatcatcct gattgtggct gtcaccctgg ctgccaattt
      601 tctcggtctc gagaccctgg gctggctcct cgagcgcttt ttgggtgtgg cggcgattgc
      661 cctgattgtc ctttttcagc cggagttgcg tcgagtgctg atgaaggtcg ggcagggtgg
      721 actcctgacc atgtacatgg atgaccggcg cgagtacctc aaggacatta ttgaggctgt
      781 cgagttcatg tcgagccgga ggctcggggc cttgatcgtt tttgaacgcg gcgtcggtct
      841 gaagaatttt gtcgagaccg ggacccagct cgaggcaagt gtttctttcg agcttttgat
      901 atccgtattt tcgaaggaat cgccgctcca tgacggtgcg gtcatcattc agcataaaag
      961 aatcagtgct gccgggtgct atcttccgtt gacgcaacgc cgcgacatcc gcaaggaata
     1021 cggaacgaga catcgcgcgg ccatcggact ctccgaggaa acggatgcgg cgattcttgt
     1081 ggtttcagaa gaaaccgggg agatctctct ggccgtggac ggtaaaattc gttccgatat
     1141 ccggcaggag aaacttgctg aaaacctgct caaggttctt ggtgaacgag agcgtgcaag
     1201 gaagaaaaag aaatgagcat cagggatctc ttcctccgga actggctgca aaagactatt
     1261 tccatgacca tcgcggtggc cctttttgtg tttgtccgca gcatgttgac ccacgaactc
     1321 cgggtacccg tccaactccg gatcaaaaac ccgccgagct atctcatgtc cgtcggaaag
     1381 atggaagaat ccatcacggt cactgtccgt ggcatcaagg ggaagatcga ctcgatcgag
     1441 ggttcgagga ttactgcctc tgtcgatatg gcggatgccg tgccggggct caacgagttt
     1501 ccggtcattc tgcattatcc gggatacagc gaggatatcc agttgaccac ctcggtgaga
     1561 agtctcagac tccagatgga ttacgccatg gaacggatga ttccattgac tccagtcatc
     1621 aagggcagtc cggctgacgg ttttgccatc catgagacaa acatccagcc ggccatgctt
     1681 ctcgttcgtg gaccggccat gatcctttcc aatctggcat cgctgcccac catgccgttt
     1741 tccgtctccg gactcaaatc cgcgatttca tctccggtcc aggtggatct ggggaacatg
     1801 aatctcgaga tcaggcagag cgggcagatt gtcctccgtt tggggattct ccccgaacgg
     1861 aagacgcggg tcttccaggc cctcgtgcct gttgttgaag gcttggggcc cgggctcaag
     1921 atggtccagc ccgaagaact tccaaaggtc aggctcatcc tttccgggga tgccgccaga
     1981 atagaccaga tcaaggagcc cgagctccgt cttgttctct cattgacaaa cctccagtcc
     2041 gggattcacg aggtcaaaat aactccggtc cttccggagg gaatccgggt caggagtgcc
     2101 gaaccgggga gcgtcgccgt tgaacttgcg gagggtatac catgaaaagc atactgggag
     2161 tcaatattga ccatgtggcc acggtccgtg aggcccgcaa gggcccgatg ccggagatcg
     2221 tgacggccgc cgggattgcg gaacagtccg gggcgcacgg gatcacaatc cacctcaggc
     2281 aggacaggcg gcatatccag gacagggatg tcgccattct caaacaggtc gtccattcca
     2341 agcttaacct cgagatgtcg atcaccgagg aaattgtcct gattgctctg gctacggttc
     2401 cggaccaggc gaccttggtt cccgaaaaca ggcaggaggt cacgaccgag ggcggtctgg
     2461 atgtcgcggg acagatcggg agggtccgcg aggtcacgca gcgcctcgcc agcgcaggga
     2521 ctctggtttc ccattttatt gatcctgaac tccggc
//
LOCUS       DAOSHE010000315         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39402_length_2552_cov_4.815378, whole genome shotgun sequence.
ACCESSION   DAOSHE010000315 DAOSHE010000000
VERSION     DAOSHE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39402_length_2552_cov_4.815378"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLD82_08190"
     CDS             <1..781
                     /locus_tag="PLD82_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG65399.1"
                     /translation="ALLFSLLYSILPPGISTLHVYRRWIDGIKPKYPVRFIALEKLNP
                     NLTEMLIAAEDGNFPVHRGIDIESMKQAWQINRQLGYYAAGASTLTQQLARTLFLFPK
                     KWMVRKYAEIWLALSMELVMPKRRIVELYVNVVEWGPGIYGIDAAARHWFKKPATALG
                     VDELARLVAILPNPLDYTPHSFWRHPDLRWRWQYLWQRFGHNQVQHRGPVQTVGQVRT
                     AHAIREPVKQRVVDETQEVKTNPAGAPGEKPPVTNLAATNR"
     gene            1148..1570
                     /gene="flgB"
                     /locus_tag="PLD82_08195"
     CDS             1148..1570
                     /gene="flgB"
                     /locus_tag="PLD82_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOG65400.1"
                     /translation="MFAGTDSFSKTSFLLERAMDASLVRRSVIADNIANVDTPYFKRG
                     EVTFESQLKRALDSEARDSMPAKLTNERHIPFRDPIDVRGVRARIQIEHESNYRNDKN
                     NVDIEKEIIDATKNMLMYNAYAQRIGGGFRMIGMLTRG"
     gene            1593..2051
                     /gene="flgC"
                     /locus_tag="PLD82_08200"
     CDS             1593..2051
                     /gene="flgC"
                     /locus_tag="PLD82_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOG65401.1"
                     /translation="MPGMFHALNVAASGMTAQRMRMDVISNNIANVNTSRTTEGGPFQ
                     RSRVILRPRNDSLRFISAVLPNRLQPTTGDGVNVVKVEKDERNPRLVYDPTHPDAIKY
                     GPKQGYVQMPNVNVVEEMVDMISASRSYEANMTMVNASKQMFTKALEIGR"
     gene            2078..2407
                     /gene="fliE"
                     /locus_tag="PLD82_08205"
     CDS             2078..2407
                     /gene="fliE"
                     /locus_tag="PLD82_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOG65402.1"
                     /translation="MKLYGNDEVSGNRVLMKAVHPDHYRIEKSKEGDDDVAKSFNSLL
                     SDSFGKVNNLLVQSDRTTQQMIVEPESVSIHTVMIAAQKAEMALSLAKAVTDRVIRAY
                     SEITNLR"
BASE COUNT          596 a          666 c          743 g          547 t
ORIGIN      
        1 ggccctgctg ttctcccttc tctacagcat ccttcctccg ggaatcagca cccttcatgt
       61 ctacagacgc tggattgacg gcatcaagcc gaagtatccg gtgcgtttta ttgccctcga
      121 aaaactcaac ccgaatctga ctgagatgtt gattgcggcc gaggatggaa attttccggt
      181 tcaccgcggg atcgacatcg agtccatgaa gcaggcctgg cagatcaacc ggcagctcgg
      241 atactatgct gccggagcca gtaccctgac ccagcaattg gcccggaccc tctttctgtt
      301 tccgaaaaaa tggatggtgc gcaagtatgc cgagatctgg ctggcactca gcatggaact
      361 ggtcatgccc aaaaggcgca ttgtggaact ctacgtcaat gttgtcgaat gggggccggg
      421 catctacggg atcgatgcgg cggcccgaca ctggttcaaa aaaccggcta cggctcttgg
      481 cgtcgatgag ctggcccgtc tggtggcgat tctgcccaac cctctcgact atacgccgca
      541 ttctttctgg cggcatcccg atcttcgttg gcgttggcaa tatctttggc agcgtttcgg
      601 ccataatcag gttcagcaca ggggacccgt ccagactgtt ggacaggtcc ggacggcaca
      661 cgccatccgt gaaccggtca aacagagggt cgttgacgaa acgcaagagg tgaagaccaa
      721 tccggcgggc gcacccggcg agaagccacc ggtgacgaac ttggccgcaa cgaaccgctg
      781 actctggccc ggattcatca cccgtcacga accgctccgg agccggtctg ttgtgtcctc
      841 tcaggagaca tgttccttct gtggtgacac ttgcataaaa aaaggcttga caaagattca
      901 gacagtgtca atacttgtac ggtgtcgacc aatggggggg gaccggaggt cggccggttg
      961 tgcctgaacg agtactttgg ggtgcctgcc aaaagcggtc gcagtctgtc gttctgacgg
     1021 atccgctcgt cttccgcatt cacctcaagc tgtccgccct ttcttccgat ggaggaggag
     1081 gggtggtgct ctccgttgga aaagcccccc tgtgttgtga ttctcagata agggaggtct
     1141 tccagcaatg tttgccggta ccgacagttt ttcaaagacc tcgtttctcc tcgagagagc
     1201 catggatgct tcattggtca gacggtcggt cattgccgac aatatcgcca atgtggacac
     1261 accgtatttc aaacggggtg aggtgacttt cgagagccag ctcaaacggg cgctggacag
     1321 cgaggccagg gattccatgc ctgccaaact gaccaacgag cggcatatcc ctttccgtga
     1381 tccgatcgat gtgcgcgggg ttcgtgcccg gatccagatc gagcacgaat cgaattaccg
     1441 gaatgacaaa aacaatgtgg atatcgaaaa agagatcatc gatgccacca aaaacatgct
     1501 gatgtacaac gcctatgccc aaaggattgg cggcgggttc cgcatgatcg ggatgctgac
     1561 cagaggataa tcgatgaaag gaggcccgga taatgccagg catgtttcac gcactgaatg
     1621 tggcagcttc aggaatgaca gcacagcgga tgcgcatgga cgtgatatcc aataatatcg
     1681 cgaatgtgaa tacctcccga acgacagagg ggggaccgtt ccagagaagc cgggtgatac
     1741 tgcgcccgcg aaatgacagt ctccgcttca tttcggcggt gttgcccaac cggctgcaac
     1801 ccacgaccgg ggacggcgtc aacgtcgtca aggtggagaa ggatgaacgg aatccccgcc
     1861 tggtctatga cccgacacat ccggatgcca tcaagtatgg tccgaagcag gggtacgtcc
     1921 agatgcccaa tgtcaatgtt gtcgaggaaa tggtcgacat gatttcggca tcccgaagtt
     1981 atgaggccaa tatgaccatg gtcaatgcat ccaagcagat gtttaccaag gcattggaga
     2041 tcgggcgctg agggcctgat ccgggagggg agttctgatg aaactgtatg gaaatgacga
     2101 ggtctccgga aaccgtgtcc tgatgaaggc ggtgcatccg gatcattaca ggatagagaa
     2161 gtccaaggag ggggatgatg atgtggcaaa gtccttcaat tccctgcttt cggactcctt
     2221 cgggaaggtc aataatctcc tggtccagtc ggacaggacg acacagcaga tgatcgtcga
     2281 gcctgaaagc gtttcgatcc acaccgtgat gatcgcggcg cagaaggcgg agatggcgct
     2341 ctccctggcc aaggctgtca cggatcgtgt gatccgtgcc tattcggaga tcaccaactt
     2401 gagataacaa tattgcggtc atgcgcggga tttgcggggg gacctgcatc gtttgcccgc
     2461 gtatgatcgt atatcaaagc agtgcctgta ttcttgggag ggaacgtatg ggcgagatgt
     2521 tgaaaaaaat cattacccag ttgaagggta tc
//
LOCUS       DAOSHE010000316         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39579_length_2545_cov_3.350602, whole genome shotgun sequence.
ACCESSION   DAOSHE010000316 DAOSHE010000000
VERSION     DAOSHE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39579_length_2545_cov_3.350602"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..931)
                     /locus_tag="PLD82_08210"
     CDS             complement(158..931)
                     /locus_tag="PLD82_08210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG65403.1"
                     /translation="MSLFLVQSSWISDMIVTFPEDQSHHLVHVLRKSCGDMLRCRQMD
                     GPVLRTRIEVADARSCTARILEVIEASEDIHPVTIVIPLLKSQRSEWIVQKVTELGCD
                     RIIPYFFERSVVRPNREKHSTRLSRIAYEACKQSERSTPPEILEEVTSFAGLGELLAS
                     EGPALRLLPWESARPSLMSVLAVHRTTAVSRVILAIGPEGGLSPDEVRNFSEAGFLPL
                     SLGQNILRSETAAITALANVVFACRDDWQSIEPVNCSHP"
     gene            1057..1581
                     /locus_tag="PLD82_08215"
     CDS             1057..1581
                     /locus_tag="PLD82_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOG65404.1"
                     /translation="MQFAPGDKVVYPLHGVGCVERLEKRDVLGNEQMYYIIRIACDDM
                     TVMIPVERSEELGLRAIVGKKDVELALDILVKKCNAGMDDDWKARFNMNKEKIRTGSI
                     FEVSEVVRNLFQRSKDKELSSSEKKLYENAYQLLVDEISLRLESERSEVEDMIAQKLE
                     EGQKRAARRKKKTV"
     gene            1578..>2545
                     /locus_tag="PLD82_08220"
     CDS             1578..>2545
                     /locus_tag="PLD82_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010284959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOG65405.1"
                     /translation="MNKRQIVRDLLLFLSVQAGVVAFSLLVEMKAFKGIPLLKSRLDV
                     VLFFCSITVVAWILVLVLRKIGGKRIFFALIGLLSGRFASEIIQIVFVPKTVQNLDLI
                     FDVFCIAVALFLSLRFARVLVSRPNFPFLGLENGKKGDIQARPKVVDTSVVIDGRIAD
                     VIDAGFIEGTFIVPEFVLTELQQIADSPDAIKRSRGRRGLDILNRMKTSANCSVVITD
                     QDFPGISEVDAKLVELALRNNAMLLTNDFNLNKIAVLKGLRVLNINELANALKSSVLP
                     GEEMTVTVIKEGKDPNQGIAYLDDGTMIVVDKGRGYINQRISVMVT"
BASE COUNT          590 a          639 c          723 g          593 t
ORIGIN      
        1 aggagttcgt ccattgaacc ggcaaaaatg cgaaacccga agaggtgtga gtatgtgcgc
       61 gacatatgag tgacgtccct tactcatgga aggcggccga gcctgtcagg cggactcccg
      121 agagtttcca aagattggct gaaatatact cacagactca tgggtgtgag caattgaccg
      181 gttcgatgga ctgccaatca tcacgacatg cgaaaacgac attggcgagc gccgtgatcg
      241 cggcggtttc gctgcggaga atgttctgtc cgagcgaaag agggaggaat cctgcctcgg
      301 agaaattccg gacttcgtcc ggcgagaggc caccttccgg tccgatggcg aggatgaccc
      361 gggatacggc cgtcgtccga tggactgcga ggacgctcat cagggagggc ctggcactct
      421 cccatggcag gagcctgagg gccggtccct ccgaagcgag aagttcaccc aagcctgcaa
      481 aacttgtcac ttcttcaaga atctcgggag gtgtgctgcg ttcagactgc ttgcaggctt
      541 cgtaggcaat cctggaaagc cgggttgaat gcttttccct gtttggccgg acgaccgagc
      601 gctcaaagaa ataggggatg atcctgtcgc atccgagttc ggtgactttt tggacaatcc
      661 actcgcttcg ctgggatttg aggaggggga tgactattgt gacggggtgg atgtcctcgg
      721 atgcttcgat cacctcaagt atccgggcgg tacaggagcg ggcatcagcc acttcgatcc
      781 tggtccgcag gaccggtccg tccatctgtc ggcaacggag catgtctccg caggattttc
      841 tgagaacgtg cacgagatgg tgactctgat cctccggaaa ggtgacgatc atgtcggaga
      901 tccagctgga ttggacaaga aaaagactca tggagaacat cctcaggcgg gaaaatgcta
      961 caatttacga taaatttatg gtatatttta ttcaagcgca agtatttcgc gcttccgccc
     1021 tgaaacggcg gcaggtcagt tcaaacggag agtacaatgc agtttgcccc tggtgacaag
     1081 gtcgtctatc cccttcacgg cgtaggttgc gtcgaacgac ttgaaaaacg ggatgtcttg
     1141 ggaaacgagc agatgtatta catcatccgc atcgcctgtg acgatatgac tgtcatgatc
     1201 cctgtcgagc ggtctgaaga gctcggactg cgtgccattg tcggcaaaaa ggatgtcgag
     1261 ctggctctcg atatcctcgt caaaaagtgc aatgccggga tggatgatga ctggaaagcc
     1321 cggttcaaca tgaacaaaga gaagatccgc accggatcca tttttgaagt ttccgaagtc
     1381 gtgcggaacc tcttccagcg aagcaaggac aaggaactct ccagttccga aaagaaattg
     1441 tacgagaacg cctaccagct tctggtggac gagatttcct tgcgactcga atccgagcgg
     1501 tccgaggttg aggacatgat tgcccaaaag cttgaagagg ggcaaaagcg cgccgcccgc
     1561 cgcaagaaga agaccgtatg aacaaacggc agattgtccg cgaccttttg cttttcctgt
     1621 ctgtccaggc gggagtggtt gctttcagtc tgctggtaga aatgaaggcc ttcaagggga
     1681 ttccccttct caaaagcaga cttgatgttg tccttttttt ctgctcgatc accgtggtgg
     1741 cttggatcct tgtcctcgtt ctgcgcaaga ttggcgggaa aaggattttc ttcgcgctga
     1801 tcggtctctt gagtgggcgc ttcgcttcgg aaatcatcca gatcgtcttt gtgcccaaga
     1861 ctgtccagaa tctcgatctc atcttcgatg ttttctgcat cgctgttgcc ctgtttcttt
     1921 ccctgcgttt tgcacgtgtt ctcgtctcac ggcccaactt cccttttctc gggcttgaaa
     1981 acggaaaaaa gggggacatc caggccagac ccaaggtcgt cgacacgagt gttgtgattg
     2041 atggccggat tgcggatgtc atcgatgcag gattcatcga ggggaccttt atcgtccccg
     2101 aattcgtgct gacggaactg cagcagatcg ctgattcccc cgatgcgatc aagcgcagtc
     2161 gcgggagacg cgggcttgat atactcaacc gaatgaagac ttccgccaat tgctcggttg
     2221 tgatcacgga ccaggatttc ccgggtatct ccgaggttga tgccaaactt gtcgagcttg
     2281 cgttgagaaa caacgccatg ctcttgacca acgatttcaa tctcaacaag attgccgtac
     2341 tcaaggggtt gcgggtcctc aacatcaatg agctggccaa tgcgctcaaa tcgagtgttt
     2401 tgcccggcga agaaatgacg gttacggtaa tcaaggaagg caaggacccc aaccagggta
     2461 ttgcctatct cgatgacggc acaatgatcg ttgtcgataa aggccgcggg tacatcaacc
     2521 agcggatttc tgtcatggtt acgag
//
LOCUS       DAOSHE010000317         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39580_length_2545_cov_3.169478, whole genome shotgun sequence.
ACCESSION   DAOSHE010000317 DAOSHE010000000
VERSION     DAOSHE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39580_length_2545_cov_3.169478"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLD82_08225"
     CDS             complement(<1..968)
                     /locus_tag="PLD82_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOG65406.1"
                     /translation="MNTDSLLARLSLSPDVAGVLSGCRSLTIAHTVEDLRTLSVCGDA
                     LSCEVAYDVPDKGRVVEAVVCRVKNGIAANYVEPYMRRRDPDSMLIGDDLPTDKERYS
                     DKYGKPFSELRNETLAWLKGQDLALFAFRAGQQDIGMNAIAIAPANAGFFALGLGLLQ
                     GVVDLRAVEGDFPVSTIVYTAPPFRHTHFNGKQIVVHNRSQKMHEIFSYNLYPGPSAK
                     KGIYGALIERGEREGWITAHASCVQVVTPYDLKLTLMHEGASGGGKSEMLEHVHREFD
                     GSILFGENTETGEKDYITLSRGCDLKPVTDDMALCHPSIQKNDGKLV"
     gene            complement(1164..>2545)
                     /locus_tag="PLD82_08230"
     CDS             complement(1164..>2545)
                     /locus_tag="PLD82_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG65407.1"
                     /translation="TASVAGGTITNVFYRFGSGSFAPVNDGGSGFATWSKSVTPSVYG
                     TQTLTVRAYDNMGQFSETTRSFVWDPVRPLAGFVSPLDGSSVTVNEPFTVTITNSDVG
                     TGVKTLKLSVNGTVIKSWSNPVGTVTHSCTLATAATHKLEVWAEDSGLTSVTNVSMIT
                     AKPELISGISSEWYKGDGTSNGTLAVSFNLAEMADVYIVVVDRATPPTAAQIIDGIDY
                     TNVNVYKAHTDTFDTGSQSWSDFVSGLTTDVANHIYLMADDGTRQSKVMYGGSFRVED
                     ANGPTAQPMTSLIFSEYIEGGGDNKVLEIYNGTGYPVQLDQYTIEVYNGGQSTPWGTP
                     WAAGTYGAVLPSGQSYVFVNSGADPAILAKKNKDVGSLDFNGDDAVVLRDPSGTIVDV
                     IGQIGFDPGDFWGDATVKTQGKILRRKKTVTGGWAPGTAAFDPTVQWNGYATINGATM
                     AAGLGTWQE"
BASE COUNT          533 a          703 c          730 g          579 t
ORIGIN      
        1 accagcttgc cgtcgttttt ctggattgag ggatggcaga gtgccatgtc gtcggtgacg
       61 ggcttgagat cacacccccg ggagagtgtg atgtaatcct tttcgcctgt ctccgtgttt
      121 tctccaaaaa gaatgcttcc gtcaaactcg cgatggacgt gttccagcat ttcgcttttt
      181 ccaccgccgg aggcaccctc atgcatgagg gtcagcttga gatcataggg ggtcacgacc
      241 tggacacagg atgcatgggc agtgatccaa ccttcgcgtt cgccgcgttc gatcaatgcc
      301 ccatagatcc ccttttttgc actggggccg ggatagaggt tgtagctgaa aatttcgtgc
      361 atcttttgac tgcggttatg gacgacgatc tgcttcccgt taaaatgcgt atggcggaac
      421 ggcggggcgg tatagacaat tgtggatacc ggaaaatcac cctcgaccgc acgaagatcg
      481 acaactccct gcagcaagcc caggcccagg gcaaagaacc ctgcattggc cggagcaatg
      541 gcaatcgcat tcatgccgat atcctgctgc cccgctctga aagcaaaaag ggccaggtcc
      601 tgtcctttga gccatgcgag cgtctcgttc cggagttctg aaaacggttt cccgtattta
      661 tcggagtagc gttccttgtc tgtgggcaga tcgtctccga tcaacatgct gtccgggtca
      721 cggcggcgca tgtagggttc gacataattg gcggcaatcc cgtttttgac acggcagaca
      781 acggcttcga cgacccggcc tttatccgga acgtcatagg ccacttcgca ggacagagcg
      841 tccccgcaga cagagagtgt ccgcagatct tccactgtgt gggcgatggt cagactgcgg
      901 cagccgctga gcacgccggc gacatccggg gaaagggaga gcctggcgag aagcgaatcg
      961 gtattcatca aatcctccat aaagagtgca gggaatcaca tcccttgggc ttgaaggtac
     1021 ttgttccgga tcatacaggc aagctgtcat gtctgtattt cttgtggagc gcgcggacag
     1081 tcaaaaacac ccgcccggag cattgggcgg gtgtttttcg cggtgatgat tgatgaaccc
     1141 tgctgtgtca atggcacaca gggttattcc tgccaagttc cgagacctgc ggccatggtt
     1201 gccccgttga ttgtggcata cccgttccac tggacggttg gatcaaaggc ggcagttcct
     1261 ggagcccatc cgccagtgac ggtttttttc cggcgcagga tcttcccctg ggtcttgact
     1321 gtcgcgtcac cccagaaatc accgggatcg aaaccgatct ggccaataac atcgactatg
     1381 gttccggaag gatcacgcag gacaacggca tcgtcgccat tgaaatcgag cgagccgaca
     1441 tccttgttct tctttgccag gatggccgga tcggctccgg aattgacaaa gacataggat
     1501 tggccggacg gcaggaccgc accataggtc ccggccgccc agggtgttcc ccatggggtc
     1561 gattgcccac cgttgtacac ttcgatggtg tactggtcga gttgtacggg atagcccgtt
     1621 ccgttataga tctcgaggac cttgttgtca ccgccacctt cgatgtactc gctgaagatc
     1681 agggaggtca tcggctgggc ggttgggccg ttggcatcct cgaccctgaa cgaaccgcca
     1741 tacatgactt tggactgccg tgtgccgtca tcggccatca ggtagatatg gttggcgaca
     1801 tcggttgtca gccccgaaac gaaatccgac caggattggg agcccgtatc aaatgtgtca
     1861 gtatgcgcct tgtagacatt gacattcgtg taatcaatgc cgtcgatgat ttgtgcggct
     1921 gttggtggtg tcgccctgtc gaccacgacg atatagacat ccgccatctc ggccaggttg
     1981 aatgagacag ccagcgttcc gttggacgtt ccatcgccct tgtaccattc ggaggagatc
     2041 ccgcttatca gctccggctt ggccgtgatc atggaaacat tggtcacgct ggtgagtccg
     2101 gagtcttcgg cccaaacttc gagcttgtgt gtggctgcag ttgcaagcgt acaactgtgt
     2161 gtcacagtcc cgacgggatt ggaccaggac ttgatgaccg tgccattgac ggacagcttg
     2221 agcgttttga caccggtgcc cacatcgcta ttggtgatcg tgactgtaaa cggttcgttg
     2281 accgtgacgg aggacccgtc cagcggactg acaaaccccg ccaacggccg gaccggatcc
     2341 cagacaaacg aacgggttgt ctcgctgaac tgacccatat tgtcataggc ccgtaccgtc
     2401 agggtctggg tgccatagac ggatggagta accgatttcg accaggtcgc aaatcccgag
     2461 ccaccatcat tgacgggcgc aaagctgcca gaaccgaacc tgtagaacac atttgtgatc
     2521 gtccctccgg caaccgaggc tgtcc
//
LOCUS       DAOSHE010000318         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39608_length_2544_cov_1.977099, whole genome shotgun sequence.
ACCESSION   DAOSHE010000318 DAOSHE010000000
VERSION     DAOSHE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39608_length_2544_cov_1.977099"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLD82_08235"
     CDS             complement(<1..920)
                     /locus_tag="PLD82_08235"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG65408.1"
                     /translation="MDESVTVVFHPLNKVASVPKKSSVLDAIREAGIQFESIYGGKGE
                     CKKCKIVHTRGDCTAGSPESTRGLSKDELQQHVCLACQTFLLGDCEFIIPVESRIDTP
                     KILLGICKDEAMGSPSVIKYRVGFDPGTLPLVGGRRSLRLLEYTGKRPQITSDFYHTL
                     TSARSPCTATISDSAGYPEIIAIEPGDTTASTYGLAVDLGTTTVVGMLFNLADTNFCA
                     QASTLNRQITYGEELVTRLAFAREPGGLEKMQLAAVESINAVLIQLTTSKGIESTSIQ
                     DICLAGNTVMQYLFYGRNPRGLEMADAVVD"
     gene            complement(1006..2451)
                     /gene="acsC"
                     /locus_tag="PLD82_08240"
     CDS             complement(1006..2451)
                     /gene="acsC"
                     /locus_tag="PLD82_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOG65409.1"
                     /translation="MSAPIQKEPKRKKSIREISPIDVYKLLPKTNCQECGESNCMAFA
                     TRVVNGELSLKDCPPLYKGEYQKDRLRLEELLAPPVRTLTFGKGNAAVTIGGKYVLQR
                     HEFTYHNPTPIAVDVHDLLSTEELGQRVQQIAQFSYPYIGRTLTLDAIAIRCVSKDPD
                     VFRSTVEKTCGMTSLPLILCSLDPAVMEAGLVAARDRKPLLYAATKDNWRGMAELAAR
                     YGCPLVLYAPNDIPLLRSLARTLLEFGIDELVLDPGTFPEGGLFGTIQNFTEIRTEAC
                     RSYDELFGFPLLGIPMTVWTGGEISEDVVKWREAAMASLLLSRYADLLIMHSLDGWVL
                     LPQLIWRFNIYTDPRKPVSVEAGVRECGKPGRDSPVLVTANYALTYFTVDSDIKAANV
                     DCFLVVIDTGGISVESAVAGRSLTPDTIAAALENYYVGSLVDHRTLIIPGLAARISGD
                     TEEATGWRVLVGPKDSSGIAGFIRDRWPEPS"
BASE COUNT          572 a          715 c          688 g          569 t
ORIGIN      
        1 ctgtctacga ccgcatcggc catctcgaga ccgcgggggt tccgaccgta aaaaagatac
       61 tgcatgaccg tatttcctgc gaggcaaata tcctgtattg acgtactctc aatacctttc
      121 gaggttgtca actggatgag aacggcattg atactttcaa ccgctgccag ctgcattttt
      181 tcaaggccgc cgggttccct ggcaaaagca agcctcgtca caagttcctc gccgtaggta
      241 atctggcggt tcagcgtcga tgcctgggca cagaaattcg tatccgcaag gttgaacagc
      301 atgcccacca ccgtggtggt tccaaggtca accgcaaggc catacgtgct ggcggtggta
      361 tcccccggtt caattgcaat gatttcaggg tatcccgcgg aatcagaaat ggttgccgtg
      421 cagggactac gagccgaagt cagggtatgg tagaaatcag aggtaatctg cggacgtttt
      481 ccggtatatt caagaagacg aagcgaacgt ctgccgccca ccagcggcag cgtgccggga
      541 tcaaaaccaa cacggtactt gataacagac ggagagccca ttgcttcatc cttgcagatg
      601 ccaagcagga tcttgggcgt atcaatcctg ctctccacgg ggatgataaa ttcacagtct
      661 cccaggagaa aagtctggca agcaagacag acatgctgct ggagctcgtc cttcgaaaga
      721 ccccgggtgc tttccgggga gcctgccgtg cagtcgccac gagtgtggac tatcttgcac
      781 tttttacatt ctcctttacc gccgtagatg ctctcgaatt ggatacctgc ttccctgata
      841 gcatccagaa ctgatgattt tttgggaaca ctggcgacct tgttgagagg atggaacaca
      901 acagttactg attcatccat gcaggcgaac gctccttatc cggatacgag taccaggttt
      961 gcaattactc cattgcagtg gcagatcgga tcctttcccc attttctagg acggctccgg
     1021 ccagcgatcc ctgatgaacc cggcaatgcc agacgagtct ttagggccga caagcactct
     1081 ccagccggtc gcctcctccg tgtcaccgct tatccgcgca gcgagacctg gaatgatcag
     1141 ggtcctgtga tcaacaagcg atccgacata gtaattctcc agcgctgcag cgatggtatc
     1201 gggcgtaaga ctccgtccgg caactgcact ctcgacgctg atgccgcccg tgtcgatgac
     1261 cacgaggaag cagtcaacgt ttgccgcttt gatgtcagaa tctacggtga aataggtcag
     1321 cgcatagttg gcggtcacca ggaccggaga atcacgaccc ggctttccac attcacggac
     1381 accagcttcg acggaaaccg gttttctcgg atcggtatag atattgaacc gccagatgag
     1441 ctggggcaga agaacccacc cgtcaagact gtgcatgatg agcagatctg cgtaccggga
     1501 gaggagcaag gatgccatgg ccgcctcccg ccatttcaca acatcttccg atatttctcc
     1561 accggtccag acagtcatag gaatcccgag tagcgggaaa ccgaacaatt cgtcgtagct
     1621 ccggcaagct tctgtccgga tttcggtgaa gttctggatc gtcccaaaga ggccgccctc
     1681 gggaaatgtg ccggggtcga gcacgagttc atcgatgcca aattcgagga gcgtgcgtgc
     1741 cagcgaacgg aggagcggga tatcatttgg cgcataaagc acgagggggc atccataccg
     1801 tgcggccagt tccgccattc ccctccagtt gtcctttgtg gctgcataca ataacggctt
     1861 ccggtcacgt gcggccacga gcccggcctc cataacagcc ggatcaaggg agcagagaat
     1921 gaggggaagg gatgtcatcc cgcatgtctt ttccacggtc gacctgaaca cgtcagggtc
     1981 ctttgaaaca caccggattg caatcgcatc aagggtaagt gtcctcccta tatatggata
     2041 ggaaaactgt gcgatctgct gcactctctg tcccagctct tccgtggaaa gaaggtcatg
     2101 gacatcaacc gctatcggtg tcgggttatg atacgtgaac tcgtggcgct ggagaacata
     2161 ttttcccccg atcgtgacag cggcattacc cttgccgaac gtcagggtcc gtacgggtgg
     2221 tgccagcaat tcctccagcc gcagacggtc cttttgatac tctcctttat aaagcggcgg
     2281 acagtctttc agtgagagtt ccccgttgac gacccgcgtg gcaaacgcca tgcagttgga
     2341 ttcaccgcac tcctggcaat tggtcttcgg gagcagcttg tacacatcga tgggactgat
     2401 ctccctgatg ctcttcttcc gctttggctc tttttgtatg ggggcgctca tgctctcacg
     2461 ccaacccagt catgtatccg ttccggcgat tccggcagcc gggacgcaag gttttggacg
     2521 acatcgcgca gggtctgcac ggcg
//
LOCUS       DAOSHE010000319         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39621_length_2543_cov_4.023714, whole genome shotgun sequence.
ACCESSION   DAOSHE010000319 DAOSHE010000000
VERSION     DAOSHE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39621_length_2543_cov_4.023714"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1181
                     /locus_tag="PLD82_08245"
     CDS             177..1181
                     /locus_tag="PLD82_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOG65410.1"
                     /translation="MHTPRAGELCGKILSADIFFPPVGNFENTAYRYWYTCQDAQRDL
                     PQRPLDTHKGRMGRVVILAGSLRYAGAAVLTAEGALASGCGLVHLAVPQRVIGLLRCL
                     PPEVILHALPDRDEGRFLPDAVATFIDLASGQDAVILGPGLGREQDTLDALKILLPRL
                     DKPCLLDADALVPGLVQAGCIPSGAILTPHVGEFSTLTGTAGLTLTRRETCARDFAET
                     HSLVLVVKDASNIVAAPGERVHWIRSGHPGLARGGSGDILAGLIGGLVSRGMESAPAA
                     RLGVWLHGAAAWRVLEAGSENFRSADLIRAIPLVMGAMLSGAPILIPSEPQSLFAKDA
                     "
     gene            1184..1717
                     /locus_tag="PLD82_08250"
     CDS             1184..1717
                     /locus_tag="PLD82_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65411.1"
                     /translation="MGQKPDPSYDPESRDPRCYYSRQERMALPNAPKPVKKNKKRIVI
                     IILLDILLLFFAWQCIFEMRKDSSRQRNEVEILRSEYHYKAVFEKAHTGYEVKLLIRM
                     TNDKAFSMPTNAMAHMTIISGGTTNSASIPSDGVSLPRPVHLFRADFPRASASGRVIV
                     RFSGAHTIPDVILEFGK"
     gene            complement(1726..>2543)
                     /locus_tag="PLD82_08255"
     CDS             complement(1726..>2543)
                     /locus_tag="PLD82_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65412.1"
                     /translation="GNPHQALAVANQAGLAHVTNSILALSTMPWIGGTLFFDCTWIES
                     PGPEIPLTLGLALTGFSSLTVEQFDINGEHRGDTALFDCAASFLASLPLPVEGLSAGA
                     AARFIIRDVYESILYGFALDLSVLWRFQLFGFGEPGFANFSLGLAIRNLGPNLAFSGG
                     DTFSDRLPTTLSLGMRWQSRESGLLVPWLSLEMTSYANEGEKNWRFGSGLILVGTLEI
                     VAGYRYGNDLAGVSLGTSITFPTVLGTVRLDYAFIPLADEIEPVHAFSLGLSL"
BASE COUNT          560 a          767 c          707 g          509 t
ORIGIN      
        1 aaccgcgttc ccccgcccga gaagccattg aagccatcaa tgcgcacaag accgagggag
       61 acgccaccgt ctacgccgta gacgttccgt ccggattgat cacttccggc gaggaacaga
      121 cccgtctggc ggtccatgcc acatatacga taacttttgg ctatcctaaa tatggtatgc
      181 acaccccgcg cgccggggag ctttgcggaa aaatactgtc tgcagatatt tttttccctc
      241 ccgttggaaa ttttgaaaat accgcttacc ggtactggta cacctgccag gatgcacaaa
      301 gggatctccc acagcgcccc ttggatacgc acaagggccg gatgggacgg gttgtcatcc
      361 ttgccgggtc cctgcgctat gctggcgctg cagtcttaac tgcggagggc gcacttgcca
      421 gcggatgcgg gctggtccat cttgcagtac cgcagcgtgt catcggcctg ttgcgttgtc
      481 tcccgccgga ggtcattctc catgcactcc cggaccgtga cgaagggcga ttccttcccg
      541 atgctgtggc cacgttcatc gatctcgcat ccggacagga tgccgtgatc ctcggcccgg
      601 ggctcggacg cgaacaggat acactggatg ctctcaaaat cctgttgccc cgactcgaca
      661 aaccctgtct gcttgatgcg gatgcccttg tgccgggcct ggttcaagca ggctgcatcc
      721 cgtccggagc cattctcacc ccccatgtcg gtgagttttc aaccctgacc ggtacggcag
      781 gactcactct gacccggcgg gaaacctgtg ccagggactt cgccgaaacg cacagcctcg
      841 tgcttgtcgt caaggacgca tccaatatcg tggctgcccc gggtgaacgt gtccattgga
      901 tccggagcgg gcatccggga cttgcccggg gtggatccgg tgatatcctc gccggactga
      961 ttggcgggct agtctcgaga ggaatggaat ccgccccggc ggcccgcttg ggagtctggc
     1021 tgcatggggc tgctgcctgg cgcgttctcg aggccgggtc cgagaatttc cggtcagctg
     1081 acctgattcg tgccatcccc ctagtcatgg gtgccatgct ctcgggagca cccatcctga
     1141 ttccgtccga accacaatct ctgtttgcaa aggatgcctg accatgggac aaaaaccgga
     1201 tccgtcgtac gaccccgaat cccgcgatcc gcgctgctat tacagtcggc aggagcgtat
     1261 ggcccttccc aatgcaccca agccagtcaa aaaaaacaaa aaacgtattg tgatcataat
     1321 acttctcgac attcttctgt tgttttttgc ctggcaatgc atttttgaaa tgcgaaagga
     1381 ttcgtcccga caacggaatg aagtcgagat cctccgctcc gaataccact acaaggctgt
     1441 cttcgaaaag gcccatacgg ggtacgaagt caaactcctc atccgcatga ccaatgacaa
     1501 ggctttttcc atgccgacca atgccatggc acacatgacg atcatcagcg gggggacgac
     1561 gaattcggca tccattccct cggacggggt ttccctcccg agaccggtcc atcttttccg
     1621 ggccgacttt ccccgtgcat ctgccagcgg gcgggtgatc gtccggttct caggggcaca
     1681 cacgatcccg gatgtcatcc tggagttcgg gaaataacac tgtccctaga ggctcaatcc
     1741 cagggaaaag gcatggacgg gctcgatctc gtccgcgagc gggatgaacg catagtccag
     1801 tctgaccgtt ccgagaacgg tcggaaaggt gatggatgtc ccgagactga ctcctgccag
     1861 atcatttcca taccggtacc cggccacgat ttccagggtt ccgacaagga tcagaccgga
     1921 cccgaaccgc cagttcttct cgccctcgtt ggcgtaactc gtcatttcaa gactgagcca
     1981 ggggacaagc aagccactct ccctggactg ccaacgcatc ccgagcgaca gagttgtcgg
     2041 aagcctgtct gaaaaggtat ccccaccgga aaacgccaga ttgggaccga gattgcggat
     2101 ggccaatccc agggaaaaat tcgcaaaccc gggttcgccg aaaccgaaaa gctgaaatcg
     2161 ccagaggact gagagatcga gggcaaaccc gtaaaggatg gattcataga catcccggat
     2221 gatgaaccgg gcagccgcac cggctgaaag cccctcgacg gggagtggca gggaagccag
     2281 aaaactggct gcacaatcaa acagggccgt atccccacga tgttccccat tgatgtcgaa
     2341 ctgctccacc gtcagagaag agaaccctgt cagcgccagc cccaaggtga gcggaatctc
     2401 aggtcctgga gattcgatcc aggtacagtc aaaaaagagg gttccaccaa tccagggcat
     2461 ggtggagagc gccaggatgc tgtttgtgac atgggccaaa ccggcttgat tggcaacagc
     2521 cagggcctga tgcgggtttc ctg
//
LOCUS       DAOSHE010000320         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39639_length_2542_cov_4.416566, whole genome shotgun sequence.
ACCESSION   DAOSHE010000320 DAOSHE010000000
VERSION     DAOSHE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39639_length_2542_cov_4.416566"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1059)
                     /locus_tag="PLD82_08260"
     CDS             complement(265..1059)
                     /locus_tag="PLD82_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65413.1"
                     /translation="MKKFLAVVLVLASVLFAACGSSISDETRSAASDHIGKISLSLSN
                     ASFSLRDAKDALAANSAYSNFAKSIADLTATEATLAKNEGYALVAQDKVLREKITKLS
                     TTIVRFTNDILGNNKDLKLEAAALDAGKRMLGVFTNARFAPIEWIRACDDASAMISDL
                     TKLMEATHKGLLEAKSGKDAGAVLVAYAASVKALSERGQMLEKRYPFFKKVPTDPILE
                     KPVAELRAAMVKLGNELKNKEKAYKDDKDFNDALKQMKDNLTTLKK"
     gene            1432..1773
                     /locus_tag="PLD82_08265"
     CDS             1432..1773
                     /locus_tag="PLD82_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG65414.1"
                     /translation="MEITSSVRDGVVVFSIVGDVSIFYKDAIDAALTEEVEKGSHLFV
                     FDFAHVSYIDSVGISFLIQAGNTAYEHGKRIMVAAANPKVRYVIELAKLDRIIGLADT
                     VDAGIAELQQG"
     gene            1839..>2542
                     /locus_tag="PLD82_08270"
     CDS             1839..>2542
                     /locus_tag="PLD82_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65415.1"
                     /translation="MRLVWTILVLLVLPLAARAELVGDASFEGVVTSLAGVPAVQRGS
                     IVSPLAPGAVILPGDSVRLAEGDEIRILLRTGRLLESRGPARLRLADEFGKEEWYSPI
                     IRDVLGERFGLYDTEMGRGPNIATNAFRQISPYNASVLFGKTECQWAAPAGYERFQVV
                     IHEEDPVSTMSKLVVVLEAGPERSLVLDSERVKLESGVPYSWRVRGWDGSAWKETDKV
                     FFRLLDRDASARIRGKL"
BASE COUNT          546 a          566 c          834 g          596 t
ORIGIN      
        1 acttccggga aggcgcgaag tggtgatatg gctggtttta tgtgcggcaa ctggacaggg
       61 agtacacaga tcggtactgg aaggcgtgaa tcccggcgca agggacttgg aacctctgtg
      121 accgtgagaa gctcgggttg gcaggatgtg tatttctgct caggtggtgc tgggaaccat
      181 cggatattcg atgtgaaact gcgtgaacca aacaaaaaac ccctcctttc ggagaggctt
      241 tattgttagt gacgtggggg caaattactt cttgagagtg gtcagattgt ctttcatctg
      301 cttgagagcg tcgttgaaat ccttgtcgtc cttgtaggcc ttctctttgt ttttgagctc
      361 attgcccagt ttgaccattg cggctctcag ctctgcaacc ggcttctcga gaattggatc
      421 ggtgggaacc ttcttgaaga agggatagcg tttttcgagc atctggccac gctcggagag
      481 ggctttgaca ctggcagcgt aggcaacaag gacggcgccg gcatccttgc cggatttggc
      541 ttccagaagg cctttgtggg ttgcttccat cagcttggtc aggtcgctga tcatggcact
      601 cgcatcgtca caggcacgga tccattcgat cggggcaaag cgggcgttgg tgaaaacgcc
      661 gagcatccgt ttgccggcgt ccaaggcggc cgcttcaagc ttgaggtcct tgttattgcc
      721 gaggatgtcg ttggtaaacc ggacaatagt tgtggagagt tttgtgatct tttcgcggag
      781 caccttgtcc tgggcgacga gggcatatcc ttcgtttttg gccagggttg cttcggttgc
      841 agtcagatcg gcgatagatt ttgcgaaatt gctgtaggcg gagtttgccg ccagtgcgtc
      901 tttggcatca cgcagagaaa aagaagcatt cgagaggctg agagatattt ttccgatatg
      961 gtcggaagca gcagagcgtg tttcgtcgct gatggacgat ccacaggctg caaacagaac
     1021 gcttgcaaga acgagtacga cggcaagaaa ctttttcatc aattcctcca tacggaatgt
     1081 agttgcgacg ggtagcctga acgcatactg agcgacgggc agttccgatt aataatactc
     1141 caagactatg cctgaaaccg aaaaaaaagt caagaggcgc agcacgcaga aacggacaga
     1201 aaacgggagg tgcaaggaac gaaaaggttc atgtgatgcg tgtttatgcc atcttccata
     1261 aaaacgcaca tcctcgaggg gcgggacgat ttcgggacaa tggagatggt cgaccggacg
     1321 gaaccggggc gtggatcggg agagagaatg aaaatagttt gacagaacgt ttttccggta
     1381 tggtatactg aggtcgagat acatgcaaga tggacgggag agtgccaagg tatggagata
     1441 accagttcgg tgcgcgacgg tgttgtggtg ttctcgatcg tcggggatgt cagcatcttc
     1501 tacaaggacg cgatcgacgc ggctttgaca gaggaagtcg aaaagggcag tcatctgttt
     1561 gtgttcgatt ttgcccacgt gagctatatc gattccgtgg gaatctcctt tctgatacag
     1621 gcgggcaata ccgcgtatga acacgggaaa cggatcatgg ttgcggcggc caatcccaag
     1681 gtgcgctacg tgatcgagct ggccaagctt gaccggatta tcgggttggc ggataccgtt
     1741 gacgcaggta ttgccgaatt gcagcagggc tgaaaatgcc actgccgggt atccggtgtc
     1801 ttggtagggg ctggttggag tggaaccgga ggtgtgccgt gagactcgtg tggacaatcc
     1861 ttgtacttct tgtgttgccg cttgcggcca gggcggagct ggtcggagac gcctcgttcg
     1921 aaggtgtggt gacctccctg gccggtgtgc cggcagtgca aagggggtcc atcgtatccc
     1981 cgttggcgcc gggggccgtg atcttgcccg gagacagtgt ccggttggcc gaaggggatg
     2041 agatcaggat cctgctcagg accgggagac tcctcgagag tcggggaccg gcgaggttgc
     2101 ggcttgcgga tgaattcggg aaagaggagt ggtactcgcc gatcattcgt gatgttctcg
     2161 gtgagcgctt cggcctctac gataccgaga tggggcgtgg ccccaatatt gcgactaacg
     2221 cctttcgcca gatttctccg tacaatgcct ccgtattgtt cgggaagaca gagtgtcagt
     2281 gggcggcgcc agccgggtat gagcgtttcc aggtggtgat acacgaggaa gatcccgtca
     2341 gtaccatgtc aaagctggtg gttgtgctgg aagcggggcc agagcgaagt ttggtgctgg
     2401 acagcgagcg ggtcaagctt gaaagtgggg tgccctattc ctggcgggtt cggggttggg
     2461 atgggtcggc ctggaaagag acagacaagg tgtttttcag gctccttgac agggatgcat
     2521 cggccaggat tcggggcaag ct
//
LOCUS       DAOSHE010000321         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_39987_length_2528_cov_3.068338, whole genome shotgun sequence.
ACCESSION   DAOSHE010000321 DAOSHE010000000
VERSION     DAOSHE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39987_length_2528_cov_3.068338"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLD82_08275"
     CDS             complement(<1..356)
                     /locus_tag="PLD82_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HOG65416.1"
                     /translation="MVWPGNRICFVFVDGIGLAPTAPTNPFSGGTMDGLEKLIGRPIL
                     AGLSARRADCLARGIDATMGVPGLPQSATGQTALFTGVNAARLCEGHMPAFPGRTLTA
                     VINQDNILKRAALAGIP"
     gene            complement(467..778)
                     /locus_tag="PLD82_08280"
     CDS             complement(467..778)
                     /locus_tag="PLD82_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG65417.1"
                     /translation="MATPVKPLTKSEIIAELAEHNGMTKKQVVAFLESLNDLAYREAK
                     KKEKFLLPGFGFLKLVKRPARTGRNPATGETIKIPAKKVVKFTLAKACKDAILPPAPK
                     K"
     gene            complement(881..>2528)
                     /locus_tag="PLD82_08285"
     CDS             complement(881..>2528)
                     /locus_tag="PLD82_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65418.1"
                     /translation="EANAAFYSGLLKNRSWLSRSQIVTLLKEQLSGDAFYGAWIVFDR
                     NQYDAQDDKYATDPMFTTSQGQFSPFWTKKDGTYTYTSISDYSDRSKPENEFYWLSKD
                     SGKPAATNPYADPDAAGILMTSLVAPIRNPEGGIVGVTGIDMVLVVLKSILNEVRPLG
                     RGRVALIAANGTWVTHPDEKLLAKDIGTNASWTQVKKAVQTGTPVFREEDSDILGEPA
                     FRIVVPVTIGNAPQRWGLLVDAPASVVLADVRELLVISLSIAAGMLIVLMFLLLLVVR
                     QTVKPLVVIAERIRDAARQVDAASSQVAQASQGLAQGAGSQAAALEETTASLTDLTNS
                     SREVTGLTEGASELMNTNIELSGTALKNLVSLTASMEEIESDSDKIGRIIKTIDEIAF
                     QTNLLALNAAIEAARAGTAGAGFAVVVDEVRSLALRTTEAAHDTQELLDGTVSRVSGA
                     AQAIRNMNDQFSGIVESATVIGEKTQAITEASREEAVRVSHIAGAASDIEKVTQQVAG
                     TAEETSATAKELSAHASVLSEMALNLTQTVLGRGAISDEG"
BASE COUNT          499 a          772 c          689 g          568 t
ORIGIN      
        1 gggatcccgg ccaaagcggc gcgcttgagg atattgtcct ggttgatgac cgccgtcagg
       61 gtcctgcccg gaaaagccgg catgtgtccc tcgcaaagac gcgccgcatt cactccggta
      121 aagagcgccg tctgcccggt ggcactctgg ggaagaccgg gcacccccat ggtggcgtcg
      181 atccccctgg caaggcagtc cgcccgccgg gcagacaggc cggccagaat cggtctcccg
      241 atcaattttt cgagtccgtc catcgtcccg cccgagaacg gattggtcgg ggctgtaggg
      301 gccagcccga ttccgtcgac aaagacaaag catatccggt ttcctggcca aaccatatcc
      361 cccccttgca cggctgccac tgcccatggc aggatcgagt actgttcacc aaaaaaaata
      421 ccccgcaggg agaacctgca gggccggtga tctcaaacca tcgggttcat tttttgggtg
      481 ccggaggaag aatggcatcc ttgcaggcct ttgccagcgt aaacttgaca accttttttg
      541 cgggaatctt gatcgtctcg ccagtggctg gattgcggcc tgtccgtgcc gggcgtttga
      601 ccagcttgag gaaaccgaat ccgggaagaa gaaatttctc cttctttttg gcttcgcgat
      661 atgcaagatc gttgagcgat tcaagaaacg ccacgacctg cttttttgtc atgccgttat
      721 gctcggcgag ttcagcaatg atctcgcttt ttgtgagcgg ttttaccggt gttgccatgc
      781 cttcacctcg tatcgatatg cgtcaaatta tgactcgtgt agcatagtca tggactttac
      841 cctctgtcaa gaagattgaa accacgcgtc aaccccgtct tcaaccttca tccgagatcg
      901 caccccgccc caaaaccgtc tgcgtaaggt tcaaggccat ctccgagagt acgctggcat
      961 gcgccgagag ttccttcgct gttgccgatg tctcttcggc tgtacccgcc acctgctggg
     1021 tcactttttc aatatcgctt gctgcacccg cgatgtggga aacccggacg gcctcttcgc
     1081 ggctagcttc agtgatggcc tgtgtcttct ccccgatgac ggtcgcactc tccacaatcc
     1141 cggaaaactg gtcgttcatg tttcggatgg cctgtgcggc tccggacacc ctgctcaccg
     1201 tcccgtccaa aagttcctga gtatcatggg ccgcctctgt cgtccgcagg gctaggctgc
     1261 gaacctcgtc gaccacaacg gcgaacccgg caccggctgt cccggccctg gcagcttcaa
     1321 tcgccgcatt caatgcgagg aggttggtct ggaacgcaat ctcgtcgatc gtcttgatga
     1381 tccgcccgat cttgtcactg tcgctttcga tttcttccat gctcgcggtc agggaaacga
     1441 gatttttgag ggcagtcccc gagagttcaa tattggtgtt catcagttcc gacgcaccct
     1501 cggtcagtcc ggtgacctcc cggctgctgt ttgtcaaatc ggtcagtgac gccgtcgtct
     1561 cttcgagagc cgccgcctgg cttccggcgc cctgtgccag accctggctg gcctgtgcca
     1621 cctgactcga ggccgcatcc acctggcggg ccgcatcccg gattctctcg gcaatgacca
     1681 ccagaggctt gaccgtctgg cggacaacaa gaagaagcag gaacatgagg actatgagca
     1741 tgcccgccgc aatcgaaaga ctgatcacaa ggagttcccg tacatccgcc aggaccacgc
     1801 ttgccggcgc gtccaccaaa agcccccagc gttgcggggc attcccgatc gtgaccggaa
     1861 ccacgatcct gaatgccggt tcgccaagga tgtccgagtc ttcctcacgg aagacaggag
     1921 tcccggtctg aaccgctttt ttgacctgcg tccaggatgc atttgttccg atatcttttg
     1981 ccaacaattt ttcatccggg tgtgtgaccc aggttccgtt ggccgcaatc aaggccaccc
     2041 gtccacgacc aagaggtcgc acctcgttga gaatggactt gagcaccaca agcaccatgt
     2101 caatccctgt cacaccgaca atcccgccct ccggattgcg gatcggggct acgaggctgg
     2161 tcatcaggat accggcagca tccgggtcag catacgggtt ggtcgcggcc ggcttcccgg
     2221 aatccttgga caaccagtag aactcgtttt cgggcttgga tctgtccgaa taatcgctga
     2281 tcgatgtata ggtgtatgtc ccgtcctttt tggtccagaa gggcgagaat tgaccctgac
     2341 tggtcgtaaa catgggatca gtggcgtatt tgtcatcctg cgcatcatac tggttgcggt
     2401 caaaaacgat ccaggcaccg taaaacgcat cccccgagag ctgctccttg aggagggtga
     2461 caatctggct cctcgaaagc cagctacggt ttttaaggag accggagtag aaggcggcat
     2521 tggcctcg
//
LOCUS       DAOSHE010000322         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_40403_length_2510_cov_4.429735, whole genome shotgun sequence.
ACCESSION   DAOSHE010000322 DAOSHE010000000
VERSION     DAOSHE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40403_length_2510_cov_4.429735"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            729..2147
                     /locus_tag="PLD82_08290"
     CDS             729..2147
                     /locus_tag="PLD82_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65419.1"
                     /translation="MNISGDLSTNVQTYTDPIESGVWFYRLKAESFIGYDSGYSATAT
                     GYPIPWPALQPPLHISSANQAAGSEVLLAWEEVNQAAGYRVYRSREEHGLYSDVSGIV
                     TGTSWVNTGITPGAVWYYKISTVNPAGKTGPLSPPVNNRIQLAAPGSAMASDGTLVNK
                     VAVLWSSVSGADHYRVYRAAAADGVSLCISPPVPAGSTQYMDETPLPGTGWYRVAAFD
                     SAGNPGLVSAADAGMPKAVPVPNPPASLVASDGTSTNGVALSWQPSADAAWYRVARAT
                     RADGDYVFISPPLTNCEFVDSSAPAKEFFYKVAAFNADGLSGLLSPADKGHKGLSDMD
                     FLIAFDTTIASSHKKMTLMHNDGMDALGKEIKNGTVAGQVLYNAAVAGLGANIVFTYA
                     GYRDEYLVLDGSYTVHASMDKSGTIDGTVTVSSPVYAGTVRYALVVTGGNASGGYWFV
                     RQQGAPVETQIAWFPSPSSQLE"
     gene            2183..>2510
                     /locus_tag="PLD82_08295"
     CDS             2183..>2510
                     /locus_tag="PLD82_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65420.1"
                     /translation="MRRIDNTRSGRRLFTKTRLLLLMLCSTLPCTTTLQALPGEYSLE
                     IGLGNSIYQSELWHYVPDAYTLNRDGGGLGLRLRQAFEVYPGISLGLAVEYLQIEERT
                     VRIGMFD"
BASE COUNT          528 a          782 c          716 g          484 t
ORIGIN      
        1 tcgcccacca acctgtcgag ggttggcgcg gctatggccc atggaacggc gcaaccatcc
       61 tcagtgcctc cgacgggagc acaagcagcg ggattctgct cacttggctt ccggtcccgg
      121 gcgcatccag ctacactgtc ttccggggaa caagctcggg cggcccatgg acgcagattg
      181 ccggaccact tgccgcgacc acatggctgg atgaaagcac ggcatacgca acacattttt
      241 ggtatcgggc tgttgccatc cgggaagatg gcgctgccgg gccgcccagt ccggcggatg
      301 agggattccg tgccttcgag gacgcgcctc cggcgccaac cggggtgaca ggaatcaacg
      361 gaggaattga cacacacaaa gtcacgtgga attcgatggg cgtagccaaa taccgcgtct
      421 ataagtccga taattatgat ggaccctgga gtcttgaggc agagcagact tcaacgacgt
      481 ggtcttcttg gtcggctttt agccgatact ggggggtatt ctaccgcatc agctgcctga
      541 gcgcatccgg caaagaaagc ccgctttctc aacctgttca aagcgctgca tggtccggaa
      601 tcaatgcgcc ggaaatgttg tctgcaagcc aaggcctatc cagaagcaga gtcacaattt
      661 cttggtcaaa agcatggaac cccaacaaat actgggtgtt ccgggcgcca tcgccagaag
      721 gaccatggat gaacataagc ggagatctct ccacaaatgt tcaaacatat accgacccga
      781 tagagtccgg agtctggttt taccgcctca aagcggaaag cttcattggc tatgattcag
      841 gatacagcgc cacagccaca gggtatccaa tcccttggcc agccctgcaa cccccgctac
      901 acatctcctc agccaaccag gcggccggtt ctgaagttct gcttgcctgg gaggaagtca
      961 accaggccgc cggctaccgt gtctaccgct cgcgcgagga acacggcctc tacagtgacg
     1021 tctccggaat cgtcaccggc acgtcctggg tgaacacagg catcaccccg ggcgccgtct
     1081 ggtactacaa gatttcgacc gtcaatccgg cgggcaagac tggtccgctt tcgcccccgg
     1141 tcaacaaccg gatccagctc gcggcccccg gctcagccat ggcctcggac gggactcttg
     1201 tcaacaaggt ggccgtcctc tggtcgagtg tttccggcgc cgatcactac cgtgtgtatc
     1261 gggctgcggc tgccgacggt gtctcgcttt gcatcagtcc gcccgtgccg gccggttcga
     1321 cacaatatat ggacgagacc ccgctcccgg gtacgggctg gtaccgggtc gccgcctttg
     1381 acagcgccgg gaatcccggt ctcgtttccg ctgccgatgc aggcatgccc aaggcggtac
     1441 ccgtccccaa tcccccggca agcctcgtcg ccagcgacgg aacaagcacc aatggggtgg
     1501 cgctctcctg gcagccctcg gccgatgccg cctggtacag ggttgcacgg gcaacacggg
     1561 cggacgggga ctacgtcttc atttctccac cgctgacaaa ttgtgaattt gtcgacagca
     1621 gtgctcccgc caaggagttt ttctacaagg tcgccgcatt caatgccgac ggactctccg
     1681 gcctgctcag cccggccgac aagggacaca agggcctttc ggatatggat tttttgatcg
     1741 cttttgatac cacgattgcc agttcccaca aaaaaatgac cttgatgcac aacgacggga
     1801 tggatgccct cggcaaagaa atcaaaaacg ggacagtcgc cggccaggtg ctctacaatg
     1861 cggctgtagc cggtctgggc gcgaacatcg tctttaccta tgccgggtat cgggacgagt
     1921 atcttgtgct ggatggctca tacacggttc acgccagcat ggataaaagc ggaacgatcg
     1981 acgggaccgt caccgtcagt tccccggtct acgccggcac ggtccgctac gccctggtcg
     2041 tcacgggagg gaatgcctcc ggcggatact ggtttgtccg gcaacaaggc gccccggtgg
     2101 agacacagat agcctggttc ccgagcccca gcagccaact ggaatgatct tccgtgcggg
     2161 aaacccaaca atggaggcat gtgtgaggag aattgacaac acccgatcag gccggagact
     2221 gttcaccaaa acaagactcc tgctcctgat gctctgctcc acgctcccct gcacaaccac
     2281 cctgcaagcg ctgcctggcg aatactcgct tgaaatcggg cttggcaatt ccatatacca
     2341 gagcgagctc tggcactatg tccctgatgc ctacaccctg aaccgggacg gcgggggact
     2401 cggtctccgt ctcagacaag cctttgaggt ctacccgggg atctcgctcg gtctcgctgt
     2461 cgagtatctc cagatcgagg agcggacggt ccgcatcggg atgtttgacg
//
LOCUS       DAOSHE010000323         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_40689_length_2498_cov_5.372084, whole genome shotgun sequence.
ACCESSION   DAOSHE010000323 DAOSHE010000000
VERSION     DAOSHE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40689_length_2498_cov_5.372084"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLD82_08300"
     CDS             complement(<1..211)
                     /locus_tag="PLD82_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65421.1"
                     /translation="MIIGKFEKNDELGTVWFRRMARVSCPSRCATISGNRPESGELMH
                     SHIDSILDEAAGPVSLSVLLSLLADR"
     gene            317..>2498
                     /locus_tag="PLD82_08305"
     CDS             317..>2498
                     /locus_tag="PLD82_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG65422.1"
                     /translation="MDINAMTLRLQEALAASQELAITRGHAEIRSLHLARAMGAQDDG
                     MFTLILSQTGVPKESFLQALDQALDRLPRLSGDGLSQATLSREASGVLAKADQERTRF
                     KDDYLSVEHVILAMAEHGESELVRIFSVNGLTPARIRQALQGVRGAHRVDTQEPESRY
                     QALQKFTIDLTERARRGKIDPVVGRDEEIRRVMQVLARRTKNNPVLLGEPGVGKTAIA
                     EGLALRIATGDVPETLAGRRLLSLDMGSLVAGTKFRGEFEDRLKGVLTEVLASDGKII
                     LFIDELHTLAGAGSAEGSIDAANMLKPALARGDLRCVGATTLKEYRKYIEKDAALERR
                     FQPVFVKEPTVDETIAILRGIKEKYEVHHGVRIQDKALVSAARLSHRYITDRFLPDKA
                     IDLVDEAASRLRIEIDSLPEEIDRLERMLIQLRIEKEALIAEHRGRERDRRLEEIGKT
                     LADGEERANTLKSRWMQEKAIITTIRQAKEKIDEYRAEAERAERAGEFNKVAEIRYGR
                     ISELERSIEAKNLELKTLQAGSPLLSEEITAESIAQVVSAWTHIPVARMLESEKDRLI
                     SMEANLAKRVIGQEQALTAVSETIRRARAGLQDATRPLGSFLFLGPTGVGKTELSKAL
                     SSFLFDDEKAMIRIDMSEYMEKHSVARLIGAPPGYVGHEEGGYLTEQVRRKPYSVVLF
                     DEIEKAHPDVFNALLQVLDDGRMTDGQGRTVDFCNTVIIMTSNLG"
BASE COUNT          566 a          752 c          723 g          457 t
ORIGIN      
        1 cgcggtcggc caggagtgaa agcaataccg acagggatac cggccccgca gcctcatcca
       61 gaatactgtc gatgtgtgag tgcatcaatt ccccggactc cggcctgttc ccggatatgg
      121 ttgcgcaacg ggatgggcac gagacccggg ccatgcgccg aaaccagacg gttcccaact
      181 cgtcattctt ttcaaattta cctataatca caccttcagt ggtgtttttc ggggagactt
      241 caggtgtatg gaaagaattg ccatttcatg cttttttact taacatttgt atatacacac
      301 tattggaggg aataccatgg acatcaatgc aatgaccctt cgtctccagg aagcactcgc
      361 cgcttcgcag gagctggcca tcacccgcgg gcatgccgag atccggagtc tccatctcgc
      421 ccgcgccatg ggtgcgcagg atgacgggat gttcaccctg atcctctcgc agacaggtgt
      481 tcccaaagag tcttttctgc aggctctcga ccaggccctg gaccggcttc cccgtctctc
      541 cggggatgga ctctcgcagg ccaccctctc ccgtgaggca agtggcgttc tcgcaaaggc
      601 cgaccaggaa cgaaccagat tcaaggatga ctacctgagc gtagaacacg tcatcctcgc
      661 catggccgag cacggggaga gcgaacttgt cagaatcttt tctgtgaatg gcctcacccc
      721 agcccggatc cgccaggcac tccagggggt ccgaggagca caccgggttg acacgcagga
      781 gccggagtcc cgctaccagg ccctgcaaaa attcaccatc gatctgaccg aacgtgccag
      841 acggggcaag atcgatcctg tcgtcggaag ggatgaggag atccgcagag tgatgcaggt
      901 tctggcccgc cggacaaaaa acaatcccgt cctgctgggc gaaccgggtg tcgggaaaac
      961 ggccatcgcc gagggcctgg ctctccggat tgctacaggt gatgtccccg aaaccctcgc
     1021 aggccgcagg ctcctctccc tcgacatggg ctccctggtc gccggaacga aattcagggg
     1081 tgaattcgaa gatcgattaa aaggcgtcct gacggaggtt ctggcttccg acggaaagat
     1141 catcctcttc atcgacgagc tccacaccct ggcaggcgcc ggttccgccg agggttcgat
     1201 agatgccgcc aacatgctca agccggccct ggcgcggggg gatctccgct gtgtgggggc
     1261 cacaaccctc aaagagtatc gcaagtacat cgaaaaggac gccgccctcg agagacggtt
     1321 ccagccagtc tttgtcaagg aaccgactgt ggatgaaacg attgccatcc tgcgcggaat
     1381 caaggaaaaa tacgaggtcc atcacggcgt ccggatccag gacaaagccc tggtctcggc
     1441 agcccggctc tcgcaccgct atatcacgga caggttcctc cctgacaagg cgatcgacct
     1501 cgtggacgaa gccgccagtc gcctgcggat cgagattgac tccctgcccg aggagattga
     1561 ccggctggag cggatgctca tccagctccg catcgagaag gaagccctga tcgccgagca
     1621 ccggggacgg gaacgggaca ggcggcttga agagatcggg aagactcttg ccgacgggga
     1681 agagcgggcc aataccctca aatcccgctg gatgcaggaa aaagccatca tcacaaccat
     1741 tcggcaggcc aaggagaaaa tcgatgaata ccgggccgag gccgaacggg ccgaacgggc
     1801 cggggagttc aacaaggtcg ccgagatccg ctatggccgg atcagcgaac ttgagcggtc
     1861 aatcgaggcc aaaaacctcg aactgaagac cctccaggcc ggctccccgc ttctctccga
     1921 ggagatcaca gccgagagca tcgcccaggt cgtctcggcc tggacccata ttccggtcgc
     1981 ccgaatgctc gagagcgaaa aggatcgttt gatctcgatg gaagcaaacc tggccaaacg
     2041 cgtgatcggc caggaacaag ccctgaccgc cgtcagtgaa accatcaggc gtgcccgcgc
     2101 cgggcttcag gatgccacac gccccctggg gtcctttctc tttctcgggc cgacaggtgt
     2161 cgggaaaacg gaactctcga aggcactctc gtccttcctc ttcgatgatg aaaaagcgat
     2221 gatccgcatc gacatgagcg agtacatgga gaagcattcc gtggcccgcc tgatcggagc
     2281 ccccccggga tatgtcggtc atgaagaagg cgggtacctg acggaacagg tccgtcgcaa
     2341 gccgtattcc gtggttctct tcgatgaaat cgaaaaagcg catcccgatg tcttcaatgc
     2401 cctgctccag gttctggatg acggacgcat gactgatggt cagggtcgga ctgtggattt
     2461 ctgcaatacc gtgatcatca tgacgagcaa tcttggac
//
LOCUS       DAOSHE010000324         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_40717_length_2497_cov_4.216626, whole genome shotgun sequence.
ACCESSION   DAOSHE010000324 DAOSHE010000000
VERSION     DAOSHE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40717_length_2497_cov_4.216626"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLD82_08310"
     CDS             <1..283
                     /locus_tag="PLD82_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOG65423.1"
                     /translation="RAAKLSSVPIGRPPRAIGKSTGDGMVAGLYYQNVGALEKIVRLL
                     KDEMGAPEPKVVATGGFANLFREGTECIDVIDADLTLKGLKIIYERQSS"
     gene            283..735
                     /gene="rimI"
                     /locus_tag="PLD82_08315"
     CDS             283..735
                     /gene="rimI"
                     /locus_tag="PLD82_08315"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOG65424.1"
                     /translation="MDLVIRPLAEADLPGVLALEEFSHPTPWSEGMFRSEMTQEISCF
                     LVVHERTSGRLVGYGGFWNMVGDGNITNVTVAPEFRRRGIGRRIVEELLERMRQQTME
                     YASLEVRDSNASAIHLYESCGFSLLGRRPGYYGDGETARIYGRRLADG"
     gene            complement(838..1236)
                     /locus_tag="PLD82_08320"
     CDS             complement(838..1236)
                     /locus_tag="PLD82_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG65425.1"
                     /translation="MKNLNFVIVEGNLVRDPDFRETEAGLPMCRFTVANNYSYKKNDQ
                     WINDVNFINIVTWSKTAENCGQYLKKGHPVRIKGRVDQSYIKREDGRSMSMVDVVAQT
                     VEFLPRVHREEPVEEAAAGLAAAEAEKKAA"
     gene            complement(1413..2321)
                     /locus_tag="PLD82_08325"
     CDS             complement(1413..2321)
                     /locus_tag="PLD82_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOG65426.1"
                     /translation="MSRKKSWILLLIAIVIAVLWFGYRSGWFDSARTQGPATTSASNP
                     AAPQNPELAGQAGLTHESPHLALGTPADATPEDEHLLLKPQYALSYKSAIVSPAWVAW
                     KIDAGSYGTVPRYEGKFFPETGLPAGLPIATHDDYSKSGFDRGHLCRSLDRTDTVENN
                     RATFSMANIIPQTHDLNGGPWLKFEDYCANLARESGKTVWVVAGGSYSTEPARIGKNR
                     VAVPTASWKVVAVLDQGIKPDLITSTSGIRLIAVEMPNTRGIIRHDWKQYRRSVDEIE
                     SRTGYDFFGRLPSRIQKDLEARTDSL"
BASE COUNT          464 a          667 c          801 g          565 t
ORIGIN      
        1 ccgggctgca aagctcagtt cggtaccgat cgggcggccg ccccgggcca tcgggaaatc
       61 gacgggggac gggatggtgg ccggactcta ctaccagaac gtcggggccc tcgaaaagat
      121 tgtgcggctc ctcaaggacg agatgggtgc acccgaaccg aaagtggttg ccacgggagg
      181 gtttgccaat ctcttccgcg aagggacgga atgtatcgac gtgatcgacg ccgatctcac
      241 actcaagggg ctgaagatca tctacgagcg gcagtcttcc tgatggatct tgtcatccgg
      301 ccgcttgccg aagctgatct gcccggggtt ttagcgctgg aagagttctc tcatccgaca
      361 ccctggtccg aggggatgtt ccgctccgag atgacccagg agatttcctg ttttcttgtt
      421 gtccatgagc gcacctcggg gagactggtc ggctatggcg ggttctggaa catggttggg
      481 gacggaaaca tcaccaatgt gacggtggcc ccggaattcc gcaggcgggg catcggccgc
      541 cggatcgttg aggagcttct ggagcggatg cggcagcaaa cgatggaata cgcctccctt
      601 gaggtccgtg attccaatgc atcagccatc catctctatg agtcgtgcgg gttctccctc
      661 ctcgggcggc gtccggggta ctatggtgac ggggaaacag caagaatcta tggcaggcgc
      721 ctggccgacg gataatattc cgattttgga attggttccg tgaaaacgga aaaccgcccc
      781 ttgcagggcg gtggcttctc ctttcgaact gagggtgttc accaaaaaaa gaccctgtca
      841 ggcagccttc ttttcggctt cggccgcggc gagtccggca gcggcttctt ccaccggttc
      901 ctcgcgatgg acgcgcggca ggaattcgac ggtctgggcg acaacatcga ccatgctcat
      961 ggagcggcca tcctcgcgct tgatatagct ctggtcgacg cggcccttga tgcggacggg
     1021 gtggcccttt ttgagatatt ggccgcaatt ttcggccgtc ttggaccagg tgacgatgtt
     1081 gatgaagttg acgtcattga tccactggtc atttttcttg taggaatagt tgttagcaac
     1141 ggtaaagcgg cacatcggaa ggccggcttc ggtctcgcga aaatcaggat ccctcacaag
     1201 atttccctcg acaatcacaa aattcagatt cttcatactg tgttctcctt tcgcttggcg
     1261 cgatctgtgt ttggggattg cttgcccctt tacccatata cacgatcggg gtgggggctg
     1321 atttttcact tttcgggaat ttttttcaaa aaaaatccgt cccgaggagg gacggagatg
     1381 acgtcggaag gattgcagga ctcccggagt cctcagaggg agtcggtccg tgcctcgaga
     1441 tccttctgga ttcgggacgg aagccgcccg aaaaagtcat agcctgtccg ggactcgatt
     1501 tcatcgacgc tgcgtcggta ctgtttccag tcgtggcgga tgatgccacg ggtgttgggc
     1561 atctcgacag ctatcaggcg aatcccgctg gttgacgtga tcaagtccgg cttgatgccc
     1621 tgatccagca cggccacgac tttccaggag gccgtcggga cggccacgcg gttcttgccg
     1681 atccgtgccg gttccgtcga atagcttcct ccagccacga cccagacggt ctttcccgat
     1741 tcacgggcca gatttgcgca ataatcttcg aacttgagcc atgggccgcc gttcaggtca
     1801 tgggtctggg ggatgatgtt ggccatggag aaggtcgccc ggttgttttc aacggtatcg
     1861 gtccggtcca acgagcggca gagatggccg cggtcgaatc cgctttttga gtagtcgtca
     1921 tgggtggcga tgggaagtcc ggctggcagc ccggtctcgg gaaagaattt tccctcgtag
     1981 cgcgggacgg taccatagga gccggcatcg attttccagg cgacccaggc cgggctgacg
     2041 atggcactct tgtacgagag ggcatactgc ggtttgagga ggaggtgctc atcctcgggg
     2101 gtggcatcgg ccggtgtccc gagtgcaaga tgggggctct cgtgggtgag tcctgcctgg
     2161 ccggccagct cgggattctg cggagccgcc gggttgctgg cactggtggt ggccggtccc
     2221 tgcgtccggg ccgagtcaaa ccagccggag cggtatccga accagaggac ggcgatgacg
     2281 atggcgatga gaagcaggat ccagcttttt ttgcggctca tgtatggtct cccgaacgtg
     2341 aagtgacgcc acaaatatac gggcagacgg ccggaaagag gggcgtcctt gtcttgaacg
     2401 ctcacggtgg atggtcccgg tttttgcgtg gtgatcgggc cggctgtgta tctttttttt
     2461 gaggaacgct ctccgatcca caatgggcgg cgtttcc
//
LOCUS       DAOSHE010000325         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_40864_length_2491_cov_3.147373, whole genome shotgun sequence.
ACCESSION   DAOSHE010000325 DAOSHE010000000
VERSION     DAOSHE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40864_length_2491_cov_3.147373"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1304)
                     /locus_tag="PLD82_08330"
     CDS             complement(354..1304)
                     /locus_tag="PLD82_08330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG65427.1"
                     /translation="MARRLFLILVCMTCSLAQDLLAAPLERIWVLNDSDPVYCAWRRD
                     VTRSRRYRGRYVRSFTFAPLRWAEYVMKHGDTLQTVAVRLGISVDAIASASGIVHVHA
                     ASPGDRLIIPNFDGISYTSTAGVRLADLSQKYDVPQEDIRRFNGSSAQWVPAGARVFI
                     PGGTMSPLEQGLFYGTAFSPPLDAILVTSGYGVRSDPFSGRQAFHGGVDLAAPAGTAV
                     KAAHEGIVEFSGWSGGYGNLIVVRHAFGYRTLYGHLSRRDVRPGMRLAMGAHLGAVGS
                     TGASTGPHLHFEIQHHGATVDPARYTSLQHRDRPAAAPVF"
     gene            1403..2215
                     /locus_tag="PLD82_08335"
     CDS             1403..2215
                     /locus_tag="PLD82_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65428.1"
                     /translation="MESARTENFFMRTVISLGSGGVNLANYILDLSALFWKSILSIFK
                     LHPYTRKVIWSNTVKQILFTGVDGLFVISSVSALLGMVIIIQAYTLLPGLGGDLVASL
                     LVLVIVRELGPILTAFIIIGRSGTAVATEIGNMKVSHEIDALHSMGIDPIRFIVAPRL
                     LGMIISMSLLGIYFDVVGILGGFVVAQLQINLPFDTFLQNMLQAIRIEDIYIGLVKNA
                     FFGIAISMVSCYQGMQVRISITEVPQRTTKAVVGSIFFCFFFNIIFTVAFYI"
     gene            2224..>2491
                     /locus_tag="PLD82_08340"
     CDS             2224..>2491
                     /locus_tag="PLD82_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG65429.1"
                     /translation="MLSLSSVNLSYAAQDVLHGISLEVMRGEFLSIVGGSGKSSLLKV
                     MGGIEPPQTGHVRYDSVDIYHVNDRKYSEMQQSSGFLFQDAALLA"
BASE COUNT          530 a          752 c          656 g          553 t
ORIGIN      
        1 ctggggatct atccccttcg cttccataaa aaacttgagg gcgccctgga aatcgcgctg
       61 tttgtacagc tcgagccccc tgccgtaggc atccaggagt ttccgtttct gttcggtcag
      121 cattgacacc tccagacacc cgacccggga gacgaggctc cgttgcaggg tgcgtccatg
      181 ttctcaaaag cattgtgaca cgaaaccagg ccgatcgtca aggatcggga tgcaggaagg
      241 ggtggaaaag atcgacatcc gctgaatccg gtccaataac agagctcacc gtcgccgatt
      301 acgctcccgg tttcccctgg cgcagtctcc ctgagacgcc ggtgcaccat gtttcaaaac
      361 acgggagccg ctgccggccg atcccggtgc tggagactcg tgtagcgagc cggatcgacg
      421 gtagcgccgt gatgctgaat ctcgaaatgc aggtgtggtc ctgtcgatgc ccctgtcgat
      481 ccaacggcgc caaggtgggc acccatggcc agtcgcatcc cgggacggac atcccttctc
      541 gaaaggtgtc cgtagagtgt cctgtacccg aaagcatgcc ggaccacaat caaattgcca
      601 tacccgccag accaaccgga gaattccacg atgccctcgt gcgcagcctt gaccgctgtc
      661 ccggcaggcg cagccagatc aactccacca tggaaggctt gccttccact gaaaggatcg
      721 ctgcgcaccc cgtatcccga cgtgaccaga atggcatcca gcggcgggga aaaagcggtt
      781 ccgtaaaaca atccctgctc gagaggggac atcgttcctc cgggaatgaa cacccgtgct
      841 cctgccggaa cccattgggc ggatgaacca ttgaaacggc ggatatcctc ctgcgggaca
      901 tcatattttt gggacagatc ggcaagacgg acgccggccg tgctcgtata gctgatcccg
      961 tcgaaattcg gaatgatgag cctgtcaccg ggggatgctg catggacatg aacgatgccg
     1021 ctggccgatg cgatcgcatc aaccgatatc ccgaggcgga ccgccacagt ctggagtgta
     1081 tccccgtgtt tcatcacata ctcggcccaa cgcaggggtg caaacgtgaa gctacggaca
     1141 taccgcccgc ggtaccgtcg tgatcgtgtg acatcgcgtc gccaggcaca atagacggga
     1201 tcactgtcat tgaggaccca gatacgctcc aagggtgccg cgaggagatc ctgggcaaga
     1261 gaacaggtca tacagaccag tatgaggaac agtcgacggg ccatgcatca gacttcggtt
     1321 cctttttgat tgaactttat cgcaggtttg tcgagaatct aaagaatgat gcgcactctc
     1381 cgcgtagagg acggaatccc ggatggaatc ggccagaaca gaaaactttt tcatgcgaac
     1441 cgtgatttcc ctggggtccg gcggtgtcaa tctggccaat tacattctcg acctctcggc
     1501 cctgttctgg aaatcgatcc tctcgatctt taaactccat ccctacaccc gcaaggtcat
     1561 ctggtccaac accgtcaagc agatcctctt caccggcgtc gacgggctct tcgtcatctc
     1621 ctcggtctcc gcacttctgg gaatggtgat catcatccag gcctacacac ttctaccggg
     1681 gctcggtgga gacctggtcg cctccctgct ggtccttgtg atcgtccgag agctcggtcc
     1741 aatcctgacc gccttcatca tcatcggtcg ttcgggaact gccgtcgcca ccgaaatcgg
     1801 aaacatgaag gtctcccacg aaatcgatgc cttgcattcg atggggattg atccgatccg
     1861 ctttatcgtc gcgccccgtc ttctcggcat gatcatctcg atgtccctct tggggatcta
     1921 tttcgatgtt gtcggcatcc tggggggatt cgtggtggca cagctccaga tcaatctccc
     1981 cttcgacacc tttttgcaga acatgctcca ggccatccgc atcgaggaca tctatatcgg
     2041 tctggtcaaa aacgcctttt tcggaatcgc catctcgatg gtcagctgtt atcaggggat
     2101 gcaggtccgg atctcgatca ccgaagtacc ccagcggacg accaaagcgg ttgtcggctc
     2161 catctttttt tgcttctttt tcaatatcat ctttaccgta gctttctaca tctgaggacg
     2221 ggtatgctct ctctttcatc agtcaacctt tcctatgctg cacaggatgt tctccacggg
     2281 atctccctcg aggtcatgcg cggagaattc ctcagcattg tgggtggttc gggcaaatcc
     2341 tccctcctca aagtgatggg tggcatcgaa ccgccgcaga caggtcatgt ccgctacgac
     2401 agtgtcgaca tctaccatgt caacgaccgg aaatacagcg agatgcaaca gtcctcaggt
     2461 tttctcttcc aggatgccgc cctcctcgcc a
//
LOCUS       DAOSHE010000326         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_40935_length_2488_cov_4.004521, whole genome shotgun sequence.
ACCESSION   DAOSHE010000326 DAOSHE010000000
VERSION     DAOSHE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40935_length_2488_cov_4.004521"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLD82_08345"
     CDS             complement(<1..443)
                     /locus_tag="PLD82_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG65430.1"
                     /translation="MMRLWWERSDLGYSDEGRLQLGGYDLASLAEAGTTPAFFYSLPR
                     ATANLQAVHAALDGTGITRHRIFYAIKANRFMPLLTAFAQSGLCGVDVCSPEEMLHAL
                     ACGFREQDISYTGTSISEADLDIICRHPQILINCDSQSTIRRLARR"
     gene            complement(595..1596)
                     /locus_tag="PLD82_08350"
     CDS             complement(595..1596)
                     /locus_tag="PLD82_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG65431.1"
                     /translation="MNANPGRRSSDRDDIFDVAGFGTIVVDQISLVPHYPAPDEKLAA
                     DEMLIQPGGPVPAALSVLSRFGRKTVLAGCIGDDLWGEWLLNSLAARMIATDFLVSVP
                     GTRTPFAQICADRTRGTRAVIHDEGSLPALTLPLLGLRCDGATPSQRASDILRQLPQA
                     RCLHIDGREHDTIPLIIDEYRKRGTKISIDCGTFRRRTIELLPLVDCIIMPASFARSV
                     FGQRPLDRLVADSHDAWLQASHIVITDGEQGSVGWCNGEIIQQGAWPVSALDSCGAGD
                     IHAGAILHTFVEDWPLSRALPFAAAAAALKCTTLGNGTLPKDLDDVFLFLDEQTQAR"
     gene            complement(1593..>2488)
                     /locus_tag="PLD82_08355"
     CDS             complement(1593..>2488)
                     /locus_tag="PLD82_08355"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOG65432.1"
                     /translation="APSSLTHLKEIDPKRIAEVARSRKFVRDIMEAREAAGKFGWTLC
                     TLATEELAKRAGCSMAEYEKQIIRACFLDVADPVARWKEIHGAAMDIKDWLGSLKIRS
                     VRIETANMDLEINLGEKRRFLGVSGHNIPSFEIFTSPDWRGARGIWYADQPSYRSGNL
                     VEGVKLEFTAGRAKVLGAHKGAEFTRQMLAMDPGACRIGEFSLTDKRFSRIDRFMADT
                     LFDENYGGKNGNSHIAVGNAYLDTYSGKQSALDKAAKTALGYNESALHWDLVNTEPKT
                     VTARLVSGASMVIYDNGQFAR"
BASE COUNT          515 a          717 c          754 g          502 t
ORIGIN      
        1 cgtctggcca gtctgcggat cgtgctctgc gagtcacagt tgatcagtat ttgaggatgt
       61 ctgcagatga tatcgaggtc agcttcggaa atggacgttc cggtgtagga gatatcctgt
      121 tcgcggaaac cgcaggcgag ggcatggagc atttcctccg gagagcagac gtcgacgccg
      181 cagagtccac tttgggcaaa ggccgtcaag agaggcataa aacgattggc cttgatggcg
      241 taaaaaatcc ggtgacgggt aatccccgtc ccgtcgagtg cggcatggac ggcctggaga
      301 ttggccgtag cccgtggaag actgtagaaa aacgcagggg tcgttcctgc ttcagccagt
      361 gaggccagat catatccacc cagttgcaaa cgtccttcgt cactgtatcc aagatcactt
      421 cgttcccacc acaggcgcat catcggttcc ccttaaacga tttcgggaaa tatagcaaga
      481 ttggacccct gccagcatcc tgtcgtctgt acatggatgt tgcgcacacg ccggcgagga
      541 aggtaggcat cccgcaggaa cccggttgaa gagattctcc cggaaaaccg gacctcaacg
      601 ggcctgagtc tgttcatcaa gaaacaggaa cacgtcatcg agatctttgg ggagcgtacc
      661 gtttcccagc gtggtgcact tgagggcggc cgccgcggca gcgaacggaa gggctcggga
      721 caggggccag tcttcgacga aggtatgcag gatggcaccc gcgtggatgt cgccggcacc
      781 acaggaatcc agtgcggaaa ccggccatgc cccctgctgg atgatctccc cgttgcacca
      841 gccgaccgag ccctgctccc cgtccgtgat gacgatgtgc gaggcctgga gccaggcatc
      901 gtgggaatca gcgacaagcc ggtcgagcgg tcgttgtccg aagacggatc gcgcaaacga
      961 tgccggcatg atgatgcagt cgaccaaggg caggagttcg atggtccgtc tccggaatgt
     1021 tccgcagtcg atgctgattt ttgtcccgcg tttcctgtac tcgtcgatga tcaggggaat
     1081 cgtatcgtgc tcccgaccat caatatggag acaccgggcc tgcggaagct gccggaggat
     1141 gtctgatgcg cgctgggacg gtgtcgcacc gtcacaccgc agtcccaaaa gcggcagggt
     1201 cagggcggga aggctgccct catcatggat gacggcgcgc gtcccccggg tgcggtcggc
     1261 acagatctgg gcaaagggtg tgcgtgtgcc cggaaccgat acaagaaaat cggtggcaat
     1321 catgcgcgca gccaggctat tgagcaacca ttcgccccaa agatcgtctc cgatacagcc
     1381 ggccaaaacc gttttccggc caaagcggga gagaaccgag agggccgccg gaacgggacc
     1441 gcccggctgg atgagcattt cgtcggcagc cagtttttca tcgggggccg ggtagtgcgg
     1501 gacgagggaa atctggtcga ccacaatcgt tccgaagccg gccacatcaa agatgtcgtc
     1561 ccggtcactg ctccgtctgc caggattcgc gttcatcgtg caaactgtcc attgtcatag
     1621 atgaccatcg atgcgccgct tacgagccgg gcggtcaccg tcttgggttc ggtattgacg
     1681 agatcccaat gcagggcgga ttcattgtat ccgagagcag ttttcgccgc tttgtcgagc
     1741 gcactctgtt tcccgctgta ggtatccaga taggcattcc cgaccgcgat gtgcgagttc
     1801 ccgttttttc cgccataatt ctcgtcgaag agcgtgtccg ccatgaagcg gtcgattctg
     1861 gaaaaccgtt tgtccgtcag cgagaattcg ccgatgcgac aggccccggg atccatggcg
     1921 agcatctggc gggtgaactc cgcacccttg tgtgcgccaa gaaccttggc ccggccagcg
     1981 gtgaactcga gtttgacacc ttcgaccagg ttcccgctcc ggtacgaggg ttggtccgca
     2041 taccagatgc cccgggcccc ccgccaatcg ggcgaggtga agatttcaaa cgaaggaata
     2101 ttgtggccac tcacgcccag aaaacggcgt ttctctccca gattgatttc cagatccata
     2161 ttggctgtct caatccggac ggaacggatc ttcagggagc cgagccagtc tttgatatcc
     2221 attgccgcgc catgaatctc cttccagcgg gcaacgggat ccgcaacatc cagaaagcag
     2281 gcccgaatga tctgtttttc gtactcggcc atgctgcatc cggcacgctt ggccagttct
     2341 tcggttgcga gcgtacagag ggtccagccg aattttccgg cggcctcgcg ggcctccatg
     2401 atatcccgga caaatttcct cgagcgggcc acttcggcaa tccgcttggg atcgatctcc
     2461 ttgagatggg tcagggatga cggtgccc
//
LOCUS       DAOSHE010000327         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41122_length_2480_cov_3.632990, whole genome shotgun sequence.
ACCESSION   DAOSHE010000327 DAOSHE010000000
VERSION     DAOSHE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41122_length_2480_cov_3.632990"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLD82_08360"
     CDS             complement(<1..193)
                     /locus_tag="PLD82_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG65433.1"
                     /translation="MSAFKLVAPYQPAGDQPRAIAELTEAIQDGVRRQTLVGVTGSGK
                     TFTMANVIRNVNKPALVLTH"
     gene            341..739
                     /locus_tag="PLD82_08365"
     CDS             341..739
                     /locus_tag="PLD82_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65434.1"
                     /translation="MKKVIISMFALVLAIGLVACGSSEDKYAKEVAPVKKAYVDGSKA
                     VIDQYKNVKTAADLKAADEAASKLDSDMNAKQAEILKKFEKVDLQKANAVSSLSNANV
                     EFDKAQGDIAAAKQAAEDVVAKAKKDEKKK"
     gene            complement(979..>2480)
                     /locus_tag="PLD82_08370"
     CDS             complement(979..>2480)
                     /locus_tag="PLD82_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG65435.1"
                     /translation="NWMTNIRDWCISRQIWWGHQVPAWYAVSETGGRVTRETPVYVGR
                     SEAEARAQAVAAHGEGVVLQQDPDVLDTWFSSWLWPFSTLGWPEKTPDLAFFYPTSVL
                     VTDMGIIFFWVARMIMAGYFCMGELPFRDVYIHGTVMDDKGRKMSKSLGNGIDPLDVI
                     REYGADALRYTMVAITPQGQNTLLSMEKFQIGSRLANKIWNASRYILMNVEGRTWPLP
                     PAEARTFEDRWILSRLQRTAESVRSAMAEFRLNDVATGIAHFFRDDFCDWYIEFTKQR
                     LYGEDEGAKAAARGVLLHVLEASLRLMHPVMPFITEEIRQRLPGVDGSIIDTRYPESD
                     ISLIDDEAEGRMGTIQAIVSGVRNVRAEMHIPPEKAVAAVIHAASPETAALVESMRAN
                     IISQARLESMTAGTDALRPPLSASVVGAGWEVWIPLEGMIDIAAERARLEKEQERLSQ
                     EVARVKGKLGNQGFVANAPEEVIAAERAKLASWEERLARLAAGLADLAG"
BASE COUNT          495 a          768 c          754 g          463 t
ORIGIN      
        1 tatgggtcaa caccagggcg ggtttgttga cgttgcggat cacattggcc atggtgaagg
       61 tcttgcccga tccggtcacc ccgaccaggg tctggcgcct gaccccatcc tgaatggcct
      121 ccgtcagttc ggcaatggcc cggggctggt ctccggccgg ttggtacggt gctaccagtt
      181 tgaaggcaga catggaacct ccctctgttg tcgtgtctgt aatataggga ttgaatctct
      241 tacgttttgt acgatttttt tccacttcaa tccgggaatg actatacttt ccggtacgcg
      301 gcggttgccg catttttata caatttatgg aggaagtatc atgaagaaag tcatcatttc
      361 catgttcgcc ctcgtgctcg ccatcggcct cgttgcctgc ggcagctccg aggacaagta
      421 cgcaaaagaa gttgcacctg tcaagaaggc ctatgttgac ggctccaagg ctgtcatcga
      481 ccagtacaag aacgtcaaga ccgctgctga tctcaaggcc gccgatgaag ccgccagcaa
      541 gctcgacagc gacatgaatg caaagcaggc tgaaatcctc aagaagttcg agaaggtcga
      601 tctccagaag gccaatgcag tcagcagcct cagcaacgcc aatgttgagt tcgacaaagc
      661 tcagggcgat atcgctgctg ccaagcaggc cgccgaagac gttgttgcca aggccaagaa
      721 ggacgagaag aagaaataat tcttctttcc cggattgaaa aaaggctgcc caaaacgggc
      781 ggcctttttt gtatgcctac aggcgcaggc agggaatccc ggcgaagatc ccgcagggat
      841 attgttctgt acgcagcatc gatacaggag gcctgcaccg gaagccacgg cagcgtgtgc
      901 atccgccgga ccagcgcggt caggcccccg ccggctccgg acgccgcagc aaaaagatca
      961 tcaaaaagcc agccgacctc agccggccag gtccgccagt cccgcggcca gccgcgccag
     1021 acgttcctcc catgaagcga gcttggcgcg ctcggcggca atcacctctt cgggggcgtt
     1081 agccacaaaa ccctgattgc cgagcttgcc cttgacacgg gcgacttcct gcgagagacg
     1141 ctcctgctcc ttttcgaggc gggcccgctc ggcggcgatg tcgatcatcc cctcgagcgg
     1201 aatccagacc tcccagccgg ccccgaccac gctggccgaa agcggcggcc gcagggcatc
     1261 cgtccccgcc gtcatgctct ccagccgggc ctggctgatg atgttggcgc gcatcgactc
     1321 cacgagggcg gccgtctccg ggctggccgc atggatcacg gccgcaaccg ctttttcggg
     1381 cgggatgtgc atctcggccc ggacgttgcg cacgccgctc acgatggcct ggatcgtccc
     1441 catccgcccc tcggcctcat catcaatcaa tgatatgtcg gattcgggat agcgtgtatc
     1501 gatgatactt ccgtcaaccc cgggcaggcg ctggcggatc tcctcggtga tgaagggcat
     1561 cacggggtgc atcaggcgca gcgaggcctc aaggacatgg agcagcacac cgcgggcggc
     1621 cgccttggcg ccctcgtcct cgccgtagag ccgctgcttg gtgaactcga tgtaccagtc
     1681 gcaaaaatcg tccctgaaga agtgggcgat cccggtggcc acgtcgttga ggcggaactc
     1741 ggccatggcc gaccggacgg attcggcggt ccgctgcagg cgcgagagga tccagcgatc
     1801 ctcgaaggtc cgcgcttcgg ccgggggcag gggccaggta cgcccctcga cattcatcag
     1861 gatgtagcgc gaggcgttcc agatcttgtt ggccaggcgc gaaccaatct ggaatttttc
     1921 catcgacagg agcgtgtttt gtccctgggg ggtgatggcc accatggtgt agcgcagggc
     1981 atccgcccca tactcgcgga tcacgtcaag gggatcgatc ccgttgccga gggacttgga
     2041 catcttgcgt cccttgtcat ccatcacggt tccatgaata tagacatccc tgaaggggag
     2101 ctcgcccatg cagaagtagc cggccatgat catccgcgct acccagaaga agatgatccc
     2161 catgtcggtc acgaggaccg aggtcgggta aaagaaggcc aggtcaggtg tcttctcggg
     2221 ccagcccagg gtggagaagg gccagagcca ggaggagaac caggtatcca gcacatccgg
     2281 gtcctgctgc aggacgacgc cctccccatg ggcggccacc gcctgggcgc gggcctcggc
     2341 ctcgctgcgt ccaacgtaga ccggggtctc gcgggtcacc cgcccgccgg tctcgctgac
     2401 ggcgtaccag gccggaacct ggtgtcccca ccagatctgg cgcgagatgc accagtcgcg
     2461 gatattggtc atccagttga
//
LOCUS       DAOSHE010000328         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41240_length_2476_cov_3.909542, whole genome shotgun sequence.
ACCESSION   DAOSHE010000328 DAOSHE010000000
VERSION     DAOSHE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41240_length_2476_cov_3.909542"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLD82_08375"
     CDS             complement(<1..666)
                     /locus_tag="PLD82_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOG65436.1"
                     /translation="MPDTVLTREIIPDALFNFVGVFRKHGKEAWLVGGTVRDFLLTGR
                     IENKDFDIATNARPKEVQRIFRKTIPTGIAHGTVTVLFEGQTFEVTTYRSDGTYSDGR
                     HPDTVCFSDTIEEDLMRRDFTVNAIACDLVGLRFVDPYGGMTDLDKRLIRAVGDPLAR
                     FREDGLRTLRACRFAGRLEFAIDEATEAAIPQTLDVFRQVATERVRDELFKIMESDRP
                     SVAL"
     gene            complement(794..1666)
                     /locus_tag="PLD82_08380"
     CDS             complement(794..1666)
                     /locus_tag="PLD82_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG65437.1"
                     /translation="MVINHNMSAINSHRVIKFKNWDLDKNVEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLRQATRNTEDGISLIQTAEGYLGESHAILQRIRELAVQAANGVY
                     TSEDRMQIQVEVSALIDEIDRIASHAQFNEMNLLTGRFARETGENRVTASMWFHIGAN
                     MDQRERVYIGTMNSQGLGLKNPVGNPETASFISISTISKANATIGTIDQALKKLSKQR
                     ADLGAYQNRLEHAAKGLMSGYENLQAAESRIRDTDMAEEMSDFVKNQILQQSGMAMLA
                     QANTKPQQVLQLLR"
     gene            complement(1792..>2476)
                     /locus_tag="PLD82_08385"
     CDS             complement(1792..>2476)
                     /locus_tag="PLD82_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65438.1"
                     /translation="AILRTPQGGVVTANRFARTGNQLWLQDFWILPPPGSPGGVRIFA
                     RFGKTVATNTLVLRNALFVTSLSNGALVSTPRTSGKLPFALDPERAETATLLYGGRSA
                     VTPLAIIEHGTSLIREGYPPIPLWQTLARQATLPFLVFLLASVAAACGWRFHQTPATR
                     SSFLLKLASVPVVGLISWFCYGTLVRLTDSLVFAAEATGIHGWAAIPVAIALLLVSNF
                     FFLARLLRS"
BASE COUNT          500 a          665 c          798 g          513 t
ORIGIN      
        1 cagggccacc gagggtctgt cgctttccat gatcttgaag agttcatccc ggacacgttc
       61 agtggccacc tggcggaaga catccagtgt ctgcggaatg gcggcctcgg tggcttcatc
      121 aatggcgaac tcgagacggc cggcaaagcg gcaagcccgc agagtccgca ggccatcctc
      181 gcgaaagcgg gcgagcggat cgccgacggc ccggatcagg cgcttgtcca gatcggtcat
      241 gccgccatag ggatcgacaa agcgcagacc gacaagatcg caggcgatcg cgttgacggt
      301 aaagtctcgg cgcatcaggt cttcctcgat cgtatcggaa aagcagacgg tgtccggatg
      361 ccgtccgtcg gaataggttc catccgaccg ataggtcgtg acttcaaagg tctggccttc
      421 aaagaggacc gtgaccgtcc cgtgggcaat tccggtcgga atggtcttcc tgaagatgcg
      481 ctgcacctct ttgggtcggg cgtttgtcgc gatgtcgaaa tccttgtttt cgatgcgtcc
      541 cgtcaggagg aagtcgcgaa cggttccgcc aaccagccag gcttccttgc catgcttgcg
      601 aaacacgccc acaaaattga agagcgcatc ggggatgatc tcgcgtgtca ggacggtgtc
      661 gggcattgct gtctcttcct caatatacca agaatctcga actgtaaaaa tacggcttcc
      721 cgtgcccggg atgagaaaaa gccgcccact gggaggcggc ctttttttac caacttcctg
      781 aatccctccg ggatcagcgg aggagctgga gaacctgctg cggcttggtg ttggcttggg
      841 caagcatggc cataccggac tgttgcagga tctggttctt gacaaagtcg gacatctctt
      901 cggccatgtc ggtgtcacgg atgcggcttt cagcagcctg caagttctcg tatccggaca
      961 tgagaccctt ggcggcatgc tcgagcctgt tctgataggc accgaggtct gcgcgctgct
     1021 tcgagagttt cttgagggcc tggtcaatcg tgccgatggt ggcattggcc tttgagattg
     1081 tactgatgct gatgaaagaa gccgtctcgg gatttccgac ggggttcttc aggccgagac
     1141 cctgcgagtt catcgtgccg atgtagacgc gttcgcgctg gtccatgttg gcgccgatgt
     1201 ggaaccacat ggaagcggta acgcggtttt caccggtttc gcgggcaaag cggccagtca
     1261 gcaaattcat ttcgttaaac tgggcatggc ttgcaatgcg gtcgatttcg tcgataaggg
     1321 ccgaaacctc aacctggatc tgcatgcggt cttcggacgt gtacacgccg ttggcggcct
     1381 gtacggcaag ttcgcgaatg cgctgcagga tggcatggga ctcgcccaga tagccttcag
     1441 ctgtctggat gagcgagatg ccgtcttccg tgttgcgtgt cgcctggcgc aagccgcgga
     1501 tctggctgcg catcttttca gatacggcca ggcctgaggc atcgtcgcca gcgcggttga
     1561 tccgcatacc ggacgagagc ttttccacgt tcttgtcaag atcccagttc ttgaacttga
     1621 tcacgcggtg cgagttaatc gcgctcatgt tgtgattgat aaccataaag agcatcctcc
     1681 ttgacggttc tcccggacct ccatgtccgg gcaatgtctt gtgtccttca taaattcgga
     1741 gaatggcaaa aattctttag aagtaaattt ttggtgtgcg cccgggcggg atcaggagcg
     1801 gagcagtcgt gcgaggaaaa agaaattcga gacaagaagg agggcaatcg caaccggaat
     1861 ggcggcccag ccgtgaattc cggtggcctc ggcggcgaag accaggctgt ccgtcagacg
     1921 gaccagggtg ccgtagcaga accaggagat caggccaaca accggcacgg aagccagttt
     1981 cagcaaaaag gagcttcgtg tggcaggggt ctggtggaaa cgccaaccgc aggccgcagc
     2041 gaccgaggcc agcaggaaga cgaggaaagg cagggtggcc tgtctggcca gtgtttgcca
     2101 gagcgggatg ggcgggtagc cctcacggat caggcttgtg ccgtgttcga tgatggcgag
     2161 tggcgtaacg gcggagcgac cgccgtagag cagggtggcc gtctcggccc gctccgggtc
     2221 cagggcaaag ggcagttttc ccgaggtgcg cggggtgctg accagtgccc cattggagag
     2281 tgaggtgaca aagagggcgt tccgcaatac cagggtgttt gtggcgaccg tctttccaaa
     2341 ccgggcaaag atgcggacgc caccggggga tcccggtggg ggcaggatcc agaagtcctg
     2401 cagccatagc tggtttccgg tccgggcaaa gcggtttgcc gtcacgacgc ctccctgggg
     2461 tgtgcgcagg atagca
//
LOCUS       DAOSHE010000329         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41376_length_2470_cov_4.369772, whole genome shotgun sequence.
ACCESSION   DAOSHE010000329 DAOSHE010000000
VERSION     DAOSHE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41376_length_2470_cov_4.369772"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLD82_08390"
     CDS             <1..260
                     /locus_tag="PLD82_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG65439.1"
                     /translation="GMAIAGVILFGVAVLFQIVTLPLEFNASSRAKKLIYEYGILGRE
                     EAYGVEKVLGAAAMTYVAAALSSITTLLYFLIRMGLLSSDD"
     gene            complement(335..706)
                     /locus_tag="PLD82_08395"
     CDS             complement(335..706)
                     /locus_tag="PLD82_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOG65440.1"
                     /translation="MQTTPAGLVRHLRKEAQRLRPVVIIGKHGLEDDVLAKIDAELTT
                     HELIKVRILDADTAHPRDSAQAIVEASRATLIQVIGKTLVLFRLNHKHPPEIPIRACG
                     LMLDRTTVRRLMYTTPKEPRV"
     gene            920..2191
                     /locus_tag="PLD82_08400"
     CDS             920..2191
                     /locus_tag="PLD82_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOG65441.1"
                     /translation="MERLIGFAGLAFFLLVAFLMSEDRKSIKLRPIIMGIVLQFLIAL
                     FVLKTPPGRMIFDFLNDVIGQMLNFTRMGADFVFGGLLKEFSFALTILPTVIFFSSFF
                     AVLYYFGILQLVVKAFAFVLRKLLGVSGAEALSNSANVFMGQTEAPLTIKPFIEKMTH
                     SELMTIMTGGMATVAGGVLAAYVSMGIDAGALIAASVMNAPAAIVLAKIIVPEKGKPV
                     TSDLKSIKVDVLDRNVLEAAARGAGEGVFLAINIAAMLLAFVAFVYLINFIFGLFGTS
                     MEQIIGTVLKPVAFVMGAPWSEAGDVAILLGKRTVLNEFIAYNDLGQFIQLGHLNAAM
                     GQGNALFSNMPFIGNTIAPAVTVLSERSITLTSYALCGFANFSSIAIQIGGIGGMAPS
                     RRGEIATLGFKCMVAGTMATFMTATVGLIIF"
     gene            complement(2268..>2470)
                     /locus_tag="PLD82_08405"
     CDS             complement(2268..>2470)
                     /locus_tag="PLD82_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG65442.1"
                     /translation="IELLKEKGYARIKIISILSSPEGIKTVSEAHPDVDIYTGCIDRC
                     LNERNYILPGLGDAGNRLFGTE"
BASE COUNT          493 a          703 c          665 g          609 t
ORIGIN      
        1 acggaatggc catcgccggc gtgatcctgt ttggcgtggc cgtcctcttc cagatcgtga
       61 ccctgcccct cgagttcaac gcctcaagcc gggccaaaaa actgatttac gaatacggga
      121 ttttgggccg cgaagaagcc tatggtgtcg aaaaagtctt gggagcagcc gccatgacct
      181 atgtggccgc agccctctcg tcgatcacga cactcctcta cttcctgatc aggatggggc
      241 tcttgtcctc ggacgattga tctccggctg catcgttcca aacaagcact accagcgatg
      301 ggagtgcttg tttttgctga acagcctcag gccatcagac gcgcggttcc ttcggggtgg
      361 tgtacatcaa acggcggacg gttgtccggt cgagcatcag tccacaagcc cggatcggga
      421 tttccggcgg gtgtttgtgg ttcaggcgaa aaaggaccaa ggtctttccg ataacttgaa
      481 tgagcgtcgc acgggaggcc tcaacgatgg cttgggccga atcgcggggg tgggcagtat
      541 ccgcatcgag aatccggacc ttgatcagtt catgtgtggt cagttcggca tcgattttgg
      601 ccaggacatc gtcctcaagg ccgtgctttc cgatgatgac aaccgggcgc aatcgctgcg
      661 cttctttgcg aaggtggcga accaggccgg cgggcgttgt ttgcatgctc tcccctgtgt
      721 gctgaaaaat ccccgggaga tgcttgcgca ctcccgggtt cgtaaatagt acacttttag
      781 ctcatcgcgc tgggtaaaaa ctcttgcatt ttctcgggca tccggtacaa tggtacggcg
      841 gtagttgtag acatatctgc ccaatatcac ccggcaatct acgtgccgga atccacgctg
      901 gtagtgaggg gagtctcaaa tggaacgact tattggattc gcaggactgg cctttttcct
      961 gttggtcgct tttttgatgt ccgaagacag gaagagcatc aagctgcgac cgatcattat
     1021 gggaattgtc ctgcaattcc tgattgcact ttttgtcctg aaaacccctc cgggtcgcat
     1081 gatcttcgat ttcctcaatg atgtcattgg acagatgctc aatttcaccc gcatgggtgc
     1141 cgatttcgtg tttggtggtt tgctcaagga gttcagtttc gccctgacca tcctgccgac
     1201 ggtgattttc ttctcctcct tctttgcggt tctctactac ttcgggattc tgcaactggt
     1261 cgtcaaagcc ttcgcctttg ttctgcgcaa gttgctgggc gtcagcggcg ccgaagcgct
     1321 ttcaaactcg gccaacgtct tcatgggaca gaccgaagcc cccctgacca tcaagccctt
     1381 tattgaaaaa atgacccact ccgaactgat gaccatcatg accggaggca tggccactgt
     1441 ggccggtggc gttttggccg cctacgtgag catgggtatc gatgccggcg ccctgattgc
     1501 cgccagtgtg atgaacgccc cggcggccat cgttctggcc aagatcatcg tccctgaaaa
     1561 aggaaagccg gtcacctcgg atctcaaatc catcaaggtg gatgtcctcg accgcaacgt
     1621 ccttgaagcg gccgcccgtg gtgccggtga gggcgtgttt ctggccatca atatcgccgc
     1681 catgctcttg gccttcgtgg ccttcgtcta cctgatcaac ttcatctttg gtctctttgg
     1741 gacctcgatg gaacagatca tcgggaccgt cctcaaaccg gtcgcctttg tgatgggtgc
     1801 tccctggtct gaagccggtg atgtcgccat cctgctcgga aagcggaccg tgctcaacga
     1861 gttcattgcc tataacgatc tcggccagtt tatacagctg ggacatctga atgccgccat
     1921 gggacaaggg aatgccttat tctcgaatat gccctttatc ggaaatacga ttgcacccgc
     1981 agtcaccgtg ctgagcgagc gctcgatcac cctgacatcc tacgccctct gcggctttgc
     2041 caacttcagc tcgatcgcca tccagatcgg cggcatcggc ggcatggccc ccagccggcg
     2101 cggtgaaatt gcgaccctcg gcttcaagtg tatggtcgcc ggaaccatgg caacattcat
     2161 gaccgcgact gtcggcctga tcatcttctg acctgcggat tccccgtgtg caaaaaaccc
     2221 tgccaagccg gcagggtttt ttgtcatgac tgaacgattc cgggaaatca ctccgttcca
     2281 aacaacctgt ttcccgcatc accaagcccc ggaagaatat aattccgctc gttgagacac
     2341 cggtcgatgc acccggtata gatatcaaca tccggatgcg cttcgctgac ggtcttgatc
     2401 ccttcagggg aagaaagaat cgagatgatt ttgatcctgg cgtagccttt ttctttgaga
     2461 agctcgatcg
//
LOCUS       DAOSHE010000330         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41598_length_2461_cov_2.926850, whole genome shotgun sequence.
ACCESSION   DAOSHE010000330 DAOSHE010000000
VERSION     DAOSHE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41598_length_2461_cov_2.926850"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLD82_08410"
     CDS             <1..1103
                     /locus_tag="PLD82_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HOG65443.1"
                     /translation="LDHIDRTLNDSFCHANIGPFATQAGRIILRHQRMASNAVPNMSI
                     KYDPETTPEDFALEAVLTALATAKPSFAHHPRFSDEFSAAGYERYAIASCYNGLPVRG
                     GSTTLVRLNLARLAETARDRDDLLTRALPDAVAAMLALMDVRVDFLVTRSGFFESSFL
                     AREGLIEFSRFTGMFGVVGLAECVNRHFAKDSGGRVFGHNDAADAFGLEIIRRIDAAV
                     RAHRAPHLEAFDGRYQLHAQVGIDSDVGVSPGCRIPIGDEPELMEHLVRSAPFHAFFP
                     SGIGDVFAFEPTAKNNPGHVLDIIRGAFSSGVRYLSLYASDADVVRITGYLVKRSELA
                     RLNRGEAVLQDTTALGRGAINNLRVLDRKLRS"
     gene            1100..1948
                     /locus_tag="PLD82_08415"
     CDS             1100..1948
                     /locus_tag="PLD82_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HOG65444.1"
                     /translation="MSTTCHVARILRSSAVDGPGNRAVVFLQGCDFDCLYCHNPETRK
                     PCNACGLCVPACRYDALRICNGEILWNPHNCRDCGACIAVCPHASSPKTRRMTVEQIL
                     SDFLPWRAFLRGITLSGGECLLQPDCTTAILETAARAGLPGLVDTNGSTPLADLPRLV
                     AAAEGFMLDIKAWDDAEHRSLTGVGNATVLANLDFLTPTGKLLEVRTVITGTFDAEQT
                     VREVARRIARAPNPVRYRIIAFRPAGVRAPGRAACQTPDATRMEHLRGIALTEGARDV
                     LLIRTP"
     gene            1983..2210
                     /locus_tag="PLD82_08420"
     CDS             1983..2210
                     /locus_tag="PLD82_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65445.1"
                     /translation="MVKIRNVVSAMFFIENSQPAELEGRMSDLVSIIRNEDTESLRIL
                     ALWFNGYLSHLANQNIGLDADAVYAQITSPM"
     gene            2220..2396
                     /locus_tag="PLD82_08425"
     CDS             2220..2396
                     /locus_tag="PLD82_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65446.1"
                     /translation="MFATKLKEHEETMISKGKIEGKIEAARQMLAEGLNEDLVSKCTG
                     LSRSDIESLRNKNN"
BASE COUNT          484 a          789 c          677 g          511 t
ORIGIN      
        1 ttctcgatca catcgaccgc acgctcaacg attcgttctg tcacgccaac atcgggcctt
       61 ttgcaaccca agccggccgg atcatcctcc gccatcagcg catggcctcc aacgccgtcc
      121 ccaatatgtc catcaaatac gatccggaga cgacccccga ggactttgct ctcgaagcgg
      181 tcctgaccgc attggccacc gccaaaccga gttttgccca ccatccgcgc ttttccgatg
      241 aattctcggc cgccgggtat gagcgctacg ccatcgccag ttgctataat ggcctgcccg
      301 tccgcggggg aagcaccacc ctggtccggc tcaatctggc ccggctggcc gaaaccgccc
      361 gtgaccggga tgatcttttg acacgggccc ttcccgacgc cgtggcagcc atgctggccc
      421 tgatggatgt gcgggtcgat tttctcgtca cccgctccgg ttttttcgag agttcctttt
      481 tggctcgaga gggcctgatc gagttttccc ggtttaccgg catgtttggc gtggtcggcc
      541 tcgcggaatg cgtcaaccga cactttgcca aagattcagg aggaagagtc ttcggccata
      601 acgacgcggc cgatgccttc ggattggaga tcatccgcag gattgatgcg gcagtccggg
      661 cacaccgcgc cccgcatctc gaggccttcg acggccgcta ccagttgcac gcccaggtcg
      721 gcattgacag cgacgtcggt gtctcgcccg ggtgccggat cccgatcggg gacgaaccgg
      781 agctgatgga gcatcttgtc cgttccgcgc ccttccacgc gtttttcccg tccgggatcg
      841 gcgatgtgtt cgccttcgag ccgacggcca aaaacaatcc gggccatgtc ctcgacatca
      901 tccggggcgc cttttcatcc ggcgtgcgct atctctcgct ctacgcctcg gatgccgatg
      961 tggtccgcat caccgggtat ctcgtcaaac gctccgaact ggcgcgcctc aaccggggtg
     1021 aggctgttct ccaggataca actgccctcg ggcgcggcgc catcaacaac ctgcgcgtcc
     1081 ttgaccgcaa gctcaggtca tgagcacaac ctgtcacgtc gcccggatcc tccgttcgag
     1141 tgccgtggac ggtcccggaa acagggccgt cgtttttttg cagggctgtg attttgactg
     1201 cctgtattgc cacaatcccg agacccgcaa gccctgcaat gcctgcggac tctgcgtccc
     1261 ggcctgccgg tatgacgccc tccggatatg caacggagag atcctctgga acccgcacaa
     1321 ctgccgggat tgcggggcct gcattgcggt ctgcccgcac gccagctcgc ccaaaacccg
     1381 tcggatgact gttgagcaaa tcctgtcaga cttccttccc tggagagcgt ttttacgggg
     1441 catcacgctc tcgggcggcg aatgcctcct ccaacctgac tgcacaacgg ccatcctcga
     1501 gacggccgcg cgcgcgggcc tgcccggact cgtcgacacg aatggctcca ccccgctggc
     1561 cgacctgcca cgtcttgtcg cagccgcaga gggattcatg ctcgacatca aggcttggga
     1621 tgatgctgaa caccgcagcc tgaccggagt cgggaatgcc actgtgctag ccaatctgga
     1681 ctttttgacc ccgacgggaa agctcctcga agtccgcacc gtgatcacgg gaacctttga
     1741 tgccgaacaa acggtgcgcg aagtcgcccg gcggatcgcc cgggctccca acccggtccg
     1801 ctaccggatc atcgccttca gaccggcggg ggtacgcgca cccgggcggg cagcctgtca
     1861 gacgcctgat gcaacccgga tggagcacct gcgcggaatc gcactgaccg aaggagcccg
     1921 ggatgttctc ctgatccgga cgccatgaga gtgtcctgat caaccagttt tcaaagagaa
     1981 gcttggtaaa aattcggaat gttgtctcgg caatgttttt tattgaaaac agtcagccgg
     2041 ccgagcttga agggcgcatg agcgatcttg tatcgattat cagaaatgaa gacacggaat
     2101 cgctcagaat tctggcactc tggttcaatg gctacctgtc tcatttggcc aatcaaaaca
     2161 tcggccttga tgccgatgct gtctatgccc aaatcacatc acccatgtag gtacgtcaca
     2221 tgtttgctac caaactgaaa gaacacgaag aaaccatgat ttccaagggc aagatcgagg
     2281 gcaagatcga ggctgcccgc cagatgcttg ctgagggtct gaatgaagat cttgtttcga
     2341 aatgcaccgg attgtcacgg tcagacatcg agtccctacg gaataaaaac aactgacaga
     2401 aggcaaccct tgtttcttcg gttacctgta ttgtgaagca acgatccttt tcgccacccc
     2461 c
//
LOCUS       DAOSHE010000331         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41631_length_2460_cov_2.730561, whole genome shotgun sequence.
ACCESSION   DAOSHE010000331 DAOSHE010000000
VERSION     DAOSHE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41631_length_2460_cov_2.730561"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLD82_08430"
     CDS             <1..502
                     /locus_tag="PLD82_08430"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG65447.1"
                     /translation="QDKKIIGMVEKAGKGLIFAINKWDTVEDNSAKHIRDLKDKIEFQ
                     LPEFAWVPVTTVSATSGRGLKKLLNLVVHVHGNASRRLETSDLNKFVQQQLKAYAPSK
                     KGKQLKIYYAVQTGTCPPAFTFFVNNATLAGVQYIHYIRNTFRKHHEYSGVPLRITLR
                     DKKETE"
     gene            499..1512
                     /locus_tag="PLD82_08435"
     CDS             499..1512
                     /locus_tag="PLD82_08435"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG65448.1"
                     /translation="MKRILSRMKVAILGAGSWGTALGITFAGMHDVLMWEFDAARATQ
                     FEHDRRNKLFLPMVGFPERLHITNDMQEAIRDARVVIFAVPSHVLREVVRKAAPYITK
                     SQYLVSVVKGVETTTLMRMTEVIASEAPSNHGVVALIGPTHAEEVARGIPTAIVAASA
                     HHSHAKTIQRYFMTPRFRIYTNSDVIGVEVGSALKNVIAIAAGICDGLKYGDNTKAAL
                     MTRGLAEMRRLGIKMGADEKTFSGLAGMGDLIVTCNSRLSRNRKVGMLLGHGRRLDDI
                     LKEMTQVAEGVINTKSALALGKRYDVELPITNAVAAVLFKNMDPAQIGQMLMTRSAKH
                     EFY"
     gene            1512..2021
                     /locus_tag="PLD82_08440"
     CDS             1512..2021
                     /locus_tag="PLD82_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG65449.1"
                     /translation="MEKQTEEGKRMDEGTPSGEQLLSKYGKGTFSIGEVSTMLGVHTH
                     TIRFWEKSFRIVIPRKESNRRRYTLADITLLKKIQRLVHNEKLSLNTARCRLEAEGVL
                     LPEPEDEEPAIQPGNEQPKPPVQARASLPVHPDKPETSPDQARAVMDSVSRELQAVIA
                     LLKSVARKS"
     gene            2080..2152
                     /locus_tag="PLD82_08445"
     tRNA            2080..2152
                     /locus_tag="PLD82_08445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2113..2115,aa:Pro,seq:ggg)
     gene            2206..>2460
                     /locus_tag="PLD82_08450"
     CDS             2206..>2460
                     /locus_tag="PLD82_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65450.1"
                     /translation="MTGLVTIIPGLVSAETDIRSLRGFALSPDFRFFPPPPTTLPLHY
                     RIEKGPLPPVTGGTVRSGWYSWEDGILSYDRPFFTGRLGMR"
BASE COUNT          563 a          744 c          659 g          494 t
ORIGIN      
        1 ccaggacaaa aagatcatcg ggatggtgga gaaggccggg aagggcttga tctttgccat
       61 caacaagtgg gataccgtcg aagacaattc cgcgaagcat atccgtgatc tcaaggacaa
      121 gatcgagttc cagcttcccg aattcgcctg ggttcccgtg acgactgtct ccgccaccag
      181 tggccgcgga ctgaaaaaac ttctcaatct cgtcgtccat gtccacggga acgcgtcccg
      241 ccggctcgaa acctcggatc tcaacaaatt cgttcaacag cagctcaagg cctacgctcc
      301 ttcaaaaaaa ggcaagcagc tcaagatcta ctacgctgtc cagacgggga cgtgcccacc
      361 ggcctttaca ttctttgtca acaatgctac actggcgggt gtgcagtaca tacactatat
      421 acgcaacacg ttccgcaagc atcacgagta cagcggagtt cccctgcgta tcaccctgcg
      481 tgacaagaag gaaacggaat gaaacgcatc ctctcgcgga tgaaggtggc cattctcgga
      541 gctggcagtt ggggaacagc gcttggaatc acattcgcgg gaatgcacga tgttctcatg
      601 tgggagtttg acgcggcccg ggccacccaa ttcgaacacg accgccggaa caagctcttt
      661 ctcccgatgg tcggatttcc cgagagactg catatcacca atgacatgca ggaggccatc
      721 cgggacgccc gggtggtcat ctttgccgtt ccttcccacg tcctccggga ggttgtccgc
      781 aaggccgctc cgtacattac aaaaagccag tatctcgtct ccgttgtcaa gggcgtggaa
      841 acaacaacct tgatgcgtat gaccgaggtc atcgccagcg aggccccctc caatcacggc
      901 gtggtagccc tgatcggtcc gacgcatgcc gaggaagtcg cccgtggaat cccgactgcc
      961 atcgttgccg cctcggcaca ccacagtcac gccaaaacca tccagcgcta tttcatgaca
     1021 ccccgcttca ggatctacac caattccgat gtgatcggcg tcgaagtcgg ttccgcgctc
     1081 aaaaacgtga tcgccatcgc cgccggtatc tgcgacggac tcaagtacgg tgacaatacc
     1141 aaggcggccc tgatgacccg tggtctggcc gaaatgcggc gtctcggcat caagatgggt
     1201 gccgatgaga agaccttctc cggtctggcc ggcatggggg atctgatcgt gacctgcaac
     1261 agccgtctca gccggaaccg caaagtcggc atgctcctcg ggcatggacg caggctggat
     1321 gacatcctca aagagatgac ccaggtcgct gagggcgtca tcaataccaa gagcgcactg
     1381 gctctcggga aacgctacga tgtcgaactc ccgatcacca atgccgtcgc cgccgtcctc
     1441 ttcaaaaaca tggatccggc ccagatcgga cagatgctga tgacccgctc cgccaaacac
     1501 gagttttatt aatggaaaaa cagaccgagg aaggaaagcg catggatgaa ggaaccccgt
     1561 cgggtgaaca gcttctgtcc aaatatggaa aaggaacctt ttccatcggg gaagtctcga
     1621 ccatgctcgg tgtgcacacc cataccatcc gtttttggga aaaatccttc cggatcgtga
     1681 ttccgcgcaa agaatccaac cgtcgccggt atactctggc ggatatcacc ctcctgaaaa
     1741 agattcaacg actggtccat aacgagaaac tcagtctcaa cacggcccgt tgtcgactcg
     1801 aggccgaggg cgtcctcctg ccagaacccg aggacgagga accggccatc caacccggaa
     1861 acgagcagcc aaagccgccc gttcaggcca gggccagtct cccggtccat ccggacaagc
     1921 cggagacaag cccggatcag gcccgggccg tcatggactc cgtttcccgt gagttgcaag
     1981 ccgtgatcgc tctgttgaag tctgtcgcac ggaagtcctg acgacggaat ccttcttttt
     2041 ttttgcttct ggcagtcttt catgctaatt ttgagggaac gggctgtagc gcagttggta
     2101 gcgtgcatga atggggttca tgaggtcgct ggttcgaatc cagtcagccc gaaaaccggt
     2161 atctcacagg taccggtttt tttattggag aagagagcag ggtgtatgac aggtctcgtg
     2221 accatcattc cgggactcgt ctcggcagag acggatatcc gctccctgcg cgggtttgcc
     2281 ctctcaccgg acttccggtt cttccctcca ccccccacaa cgctccccct gcattaccgg
     2341 atcgagaagg gaccactccc acctgtcacc ggaggaacgg tccgttccgg ttggtactcc
     2401 tgggaagatg gcatcctctc ctatgaccgg ccgtttttca ccggccgtct gggaatgcgt
//
LOCUS       DAOSHE010000332         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41690_length_2457_cov_3.641549, whole genome shotgun sequence.
ACCESSION   DAOSHE010000332 DAOSHE010000000
VERSION     DAOSHE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41690_length_2457_cov_3.641549"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /locus_tag="PLD82_08455"
     CDS             complement(<1..2402)
                     /locus_tag="PLD82_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65451.1"
                     /translation="MPHLKTALTISGRLEYPEPMQENLYKDLLDAAPWAWARHRFVRD
                     GAGAASDYVFLEVNRTFCEMTGFVRDAIIDRPVSQVIPGIRTGGFDWVAHYDGIVRSG
                     QPVEFEQYVQALDKWFRVKVLPGEDDTFTTFFVDITREHARIDELDRFFSVNLDLLCI
                     ADLEGNFIKINAAWEDILGYTVGELTGKRFLDFVHPDDMAATLDAMSTLAGQNDVLNF
                     TNRYRCRDGSWRWIEWRSHPHGKLIYAAARDITERVSKQALIESLSRQYELVFDGTLD
                     AIFLVQCCEDGAWRYVRTNRAHQNDTGIDGATINGKTPQELLGDALGNAVAASYDRCL
                     AGNRPVTYEESPVLPAGQRTWLTTLTPACSSDGTRYLIGSSIDITERKNIEGRLKEQT
                     ALLEGLLDSIPDIVFFKDLEGTYLGCNTLFCELVGRHRSDVIGYTDYDFFPREDAEGF
                     RRHDVLIQESGEECSTEEWLVFPEGKRILFDMLKAPLRDVAGRTIGLLGVGRDITDRK
                     RIEAELVREKERVNNVIEGTNIGTWEWNVQTGETVFNERWAAIVGYTLDDLAPVSIKT
                     WSDLVHPEDSMRSQKELERVFSQQLEFYDCECRMRHREGHWVWVHDRGKVLRWTDDGR
                     PLIMYGTHMDISLRKEAEAAMLDARRLAEEASQAKSRFLANMSHEIRTPLNGVIGFAD
                     LMSRTRLDPLQDQYMKNIVHSANSLMDLINDILDFSKIEAGRMELDPEMISVSGLCED
                     TADFVKLAAHSKGLELVLLIDPLLPGQILVDPVRLRQILVNLLGNAVKFTERGAVTLE
                     AG"
BASE COUNT          531 a          728 c          673 g          525 t
ORIGIN      
        1 aacccggcct caagagtaac cgcccccctc tcggtaaact tgacggcgtt gcccagcagg
       61 ttgaccagaa tctggcgcaa gcggaccgga tcgacaagga tttgtccggg gagcagcgga
      121 tcgatcagga gaaccagctc aagccctttg gagtgtgccg ccagcttgac aaaatcggca
      181 gtatcttcgc acaggcccga tacggagatc atctcgggat caagttccat ccgaccggct
      241 tcgatttttg aaaaatcgag tatgtcgttg atcagatcca tcaacgagtt ggccgagtgg
      301 acaatgtttt tcatgtactg atcctgcaac ggatcgagcc tggtccggga catgagatcg
      361 gcaaacccga tcacgccgtt gagcggcgtc cggatctcgt ggctcatgtt ggccaaaaac
      421 cggctcttgg cctgggatgc ctcttcggcc agcctgcgtg catcgagcat ggccgcctcg
      481 gcctccttgc gcaggctgat atccatgtgt gtgccgtaca tgatcagtgg ccggccgtcg
      541 tcggtccagc gcagcacctt ccccctgtca tgtacccaga cccagtgccc ttcccggtgc
      601 cgcatgcggc actcgcaatc gtaaaactcc aactgttgtg aaaaaacccg ttccagttcc
      661 ttttgggaac gcatgctgtc ttcgggatga acaagatcgc tccaggtctt gatggaaacg
      721 ggagcgagat catcgagggt gtacccgacg atggccgccc agcgctcgtt gaacaccgtc
      781 tcgccggtct ggacattcca ctcccaggtt ccgatgttgg tgccctcgat gacgttgttg
      841 acccgctcct tctcgcgcac cagctcggcc tcgatccgtt tgcggtccgt gatatcacgt
      901 ccgacgccga gaagtccgat agtcctgccg gccacatccc gcaagggagc tttaagcatg
      961 tcaaagagaa tcctttttcc ctcggggaaa accagccact cctcggtact gcactcctcg
     1021 ccgctctcct gtatcaggac atcatgccgt ctgaatcctt ctgcatcctc gcgggggaaa
     1081 aaatcgtagt ccgtatatcc gatgacatca ctccggtgtc ggcccacgag ctcgcaaaaa
     1141 agtgtattgc accccagata cgttccctca agatccttga agaaaacgat atcaggtatg
     1201 gaatcaagga gaccctcaag aagcgccgtc tgctctttca gccggccctc gatatttttc
     1261 cgctcggtga tgtcgatcga agagccgatc agatagcggg tcccatcact ggaacaggcc
     1321 ggggtgagcg tcgtgagcca agtccgctgg ccggccggaa gaaccggact ctcctcgtac
     1381 gtgacaggcc tgttcccggc caggcagcga tcgtagctgg ctgcaaccgc gtttcccaat
     1441 gcatcgccaa ggagttcttg cggagttttt ccattgatgg tcgccccgtc aattccggta
     1501 tcgttttgat gggcgcgatt tgtccggaca tagcgccagg caccgtcttc gcaacattgt
     1561 accagaaaaa tcgcatcgag ggtcccgtca aaaaccagct cgtactgccg ggaaagggat
     1621 tcgatcaggg cctgtttgct gacacgctcg gtaatatcgc gggcagccgc gtagatcagc
     1681 ttgccatggg gatgggagcg ccattcgatc cagcgccagc tcccgtcacg acagcggtaa
     1741 cggtttgtga aattgaggac atcgttttga ccggccagcg ttgacatggc atccagagta
     1801 gccgccatgt catcgggatg gacaaagtca agaaagcgtt tgcccgtgag ttcgccgacc
     1861 gtgtagccga ggatgtcctc ccaggctgcg ttgatcttga taaaattccc ttcgaggtca
     1921 gctatgcaga ggagatcgag attgaccgaa aagaagcggt ccagttcgtc aatgcgggca
     1981 tgttcgcggg tgatgtccac aaaaaacgtg gtaaacgtgt catcctcgcc gggcaggacc
     2041 ttgacgcgaa accatttatc aagtgcctgg acatactgct caaactccac cggctgtccg
     2101 ctgcggacaa taccgtcata atgggcaacc cagtcaaatc cgcccgtacg gattccggga
     2161 atcacctgac tgacgggccg atcgatgatc gcatcccgga caaagcctgt catctcgcaa
     2221 aatgtccggt tgacctcaag gaaaacgtaa tcgctggcgg ctcccgcccc gtcacggacg
     2281 aatcgatggc gagcccaggc ccagggggcc gcatcaagca aatccttgta gaggttctcc
     2341 tgcattggct caggatactc cagtcgtccg gagattgtca acgcggtttt cagatgaggc
     2401 atgtgcgctg cctcagcgcg ggtattcaat ccacacgcta ctcgacgatg tcatccg
//
LOCUS       DAOSHE010000333         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41875_length_2449_cov_4.571429, whole genome shotgun sequence.
ACCESSION   DAOSHE010000333 DAOSHE010000000
VERSION     DAOSHE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41875_length_2449_cov_4.571429"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..>2449)
                     /gene="dnaE"
                     /locus_tag="PLD82_08460"
     CDS             complement(384..>2449)
                     /gene="dnaE"
                     /locus_tag="PLD82_08460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG65452.1"
                     /translation="DPRNKSKSLLEKAIDAEPRLREIREKGGVYETLFRNALKLENVI
                     RGTGKHAAGVVIAGRPLVEYLPLYKDSKSNQVITQYDGPHLEKVGLLKMDILGLQNLT
                     TMEECRHLIHDTRGIDLDFSTIPFEDEKAFTLLQRGQSLGVFQLDSPGMQQLLRNMKP
                     DRFDEIIALIALYRPGPLNMGMHTKFCDRKAGREPVEYILPETEPVLKDTYGIIIYQE
                     QVMQISQAVGGFSMAEADELRKAMGKKKMDIVDKKRNDFLQGAKEKKINTAKAEELYN
                     LMAQFAEYGFNKSHSAAYAIISWQTAWLKANYPLEYMAALLTSQKDSPDKLELYIRET
                     QSMGITILPPDVNFSTVNFSVENNAIRYGLSAIKGIGLAASEAVVRARDSKPFFHDLV
                     EFCAAVDLRAVTKRVLETLVSCGAFASTGKKRSVLFESIDASVDKGSRMRVDAERGQI
                     NFFDDFDEDSGTESGSGPALSEDIREWCEDKLLSLEKDLLGTYITGHPFRRYLRTWKR
                     LGMTGSRRHASLEANKHVSVGGIVRGIEERLTKEGKAFGIVTLEDPDGNWDFMLFSNN
                     WEKYKSFFVPGTALCIQVSVSKQPGRDKPMFHPQQIRFLDDLVVHDLHITIGPELSDS
                     DLFAIREELLSDGNRGDSAVYFHIEEPGRSVTVQASQSIHVKADEQLLGFLRSKPGVR
                     EVSLE"
BASE COUNT          490 a          642 c          755 g          562 t
ORIGIN      
        1 cggatcggcg tatttcaggg gtgtcacgca ggtataaaaa acaggcggtc tgaaccagac
       61 cgcctgtcta tcgggatggt agggtgttgt gtttgtgtcc acgggcaacg cccgcaatac
      121 atctacctat aaagcaagca gcgtgccaag gtgtgagttt gttccaagaa aatgataaaa
      181 atcatcaaaa aagcccgatt attgtgaaaa atcaagtatt tttgggtgct gttgtccggg
      241 attcgggtaa aaaacgcgat ttttccacaa aaatttagta ccaggactgg tcagcccggt
      301 gtacggatgc gacgggccac tgtcgaaatg gcacacttcg cccggagtat gccgctccca
      361 tgccaaaatg acacaactcg cttctattcg agcgagacct cccggacgcc tggctttgat
      421 cggagaaacc cgaggagctg ctcgtcggct ttgacgtgga tcgactggct ggcctggacg
      481 gtgacactcc ggcccggttc ctcgatgtgg aaatagacgg cggagtcgcc ccggttgcca
      541 tccgagagca attcttccct gatggcaaag agatcgctgt cggacaactc cggcccgatg
      601 gtgatgtgca gatcgtgtac gaccaggtca tcgagaaacc ggatctgctg ggggtggaac
      661 atcggtttgt cgcgcccggg ttgtttggag acggaaacct ggatgcagag ggcggttccg
      721 gggacgaaaa aggatttgta cttttcccag ttgttcgaaa acagcatgaa gtcccagttc
      781 ccgtccggat cctcgagggt gacgatgcca aaggccttcc cctccttggt gagccgttct
      841 tcaatacccc ggacgatgcc acccacggac acgtgtttgt tggcttccag ggacgcgtgc
      901 cgtcttgacc ccgtcattcc caggcgtttc cacgtccgca gatagcgacg gaagggatgc
      961 ccggtgatat aggtgcccaa aagatccttt tcgagggaaa ggagtttgtc ctcgcaccac
     1021 tcccggatat cctcggaaag cgcaggcccg cttcccgatt cggtgccgga gtcttcgtca
     1081 aaatcatcga agaaattgat ctggccgcgc tcggcgtcga cccgcatccg gctgcccttg
     1141 tcgacagaag cgtcgattga ctcgaagagg acgctccgtt tttttccggt cgaggcaaag
     1201 gccccgcagg agacgagggt ctcgagaacc cgtttggtga cggcacgcag gtcgactgcc
     1261 gcgcaaaatt cgaccagatc gtggaagaat ggcttgctgt cccgtgcccg gacgacagcc
     1321 tccgaggcgg ccagtccgat gcccttgatg gcggagagcc cgtagcggat cgcgttgttc
     1381 tcgacggaga aattgacggt gctgaaattg acatccgggg ggaggatcgt gattcccatg
     1441 gactgggtct cgcggatgta gagttccagt ttgtccggcg aatccttttg cgaggtgagg
     1501 agcgcagcca tgtactcgag ggggtaattg gccttgagcc aggctgtctg ccaggagatg
     1561 atggcatagg ccgcgctgtg ggatttgttg aagccgtact cggcgaactg ggccatcagg
     1621 ttgtagagct cttccgcttt ggcggtattg atcttttttt ccttggctcc ctggaggaag
     1681 tcgttgcgct ttttgtcaac gatgtccatc ttcttctttc ccatggcctt gcgcaactca
     1741 tcggcttcgg ccatcgaaaa tcctccgact gcctgcgaaa tctgcatcac ctgttcctga
     1801 tagatgatga tcccgtaggt gtccttgaga accggctcgg tttcgggaag gatgtattcc
     1861 acaggctccc gacccgcttt gcggtcgcag aatttcgtgt gcatgcccat attgaggggg
     1921 ccggggcggt agagggcgat cagggcgatg atctcgtcaa accggtccgg cttcatattg
     1981 cgcaggagtt gctgcatccc gggagagtcg agctggaaga cgcccaggga ttgtccgcgc
     2041 tggagaaggg taaaggcctt ttcatcctcg aaggggatgg tcgaaaaatc cagatcgatc
     2101 ccccgggtat catggatcag atgccggcat tcctccatcg tggtcaggtt ctgcaggccg
     2161 aggatgtcca tcttgaggag tccgaccttc tcgaggtggg gaccgtcgta ctgcgtgatg
     2221 acctggttgg acttgctgtc cttgtagagc gggagatatt cgaccagggg gcggccggcg
     2281 atgacaacac cggcggcgtg tttcccggtt ccgcggatca cgttttcgag tttgagagca
     2341 ttcctgaaga gggtttcata gacgccgccc ttctcccgga tctcccggag gcggggttca
     2401 gcgtcgatgg ccttctcgag gagggacttg gatttgttgc ggggatcgg
//
LOCUS       DAOSHE010000334         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41886_length_2449_cov_2.644110, whole genome shotgun sequence.
ACCESSION   DAOSHE010000334 DAOSHE010000000
VERSION     DAOSHE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41886_length_2449_cov_2.644110"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PLD82_08465"
     CDS             <1..1536
                     /locus_tag="PLD82_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG65453.1"
                     /translation="DLTLAGMKHTLDIREIRVSHGRLDLVGPGGILRTIPLDDSGNLS
                     LKFYGPFRKLYNAHKHNFFLASDIINASIEPESFTNVRPEYFKNKIVFLGTDAPGLKD
                     IRPNPFSNTDGGVYLYSTAMENILSSDYITEAYDEWLTILLILLLSMITGYVGARLSV
                     IRGVLASLGMILVYSFVALLLFSSASILIEMASVLTATLLSFLVGAVRNYYLESRQKH
                     FIQGAFSQVLAPAILEKLIEDPSSLTLGGDSKELTIFFSDLAGFTTLSEALGSPQRLV
                     SILNEYLTAMADIIMDYDGYVDKYEGDAIMAFWGAPVDDPNHAWKGCYAAIDNQIKLA
                     ELQERFVSMGLPGGLAVRIGLNTGVAVAGMMGSMKKLNYTIIGDPVNLASRLEGANKQ
                     YGTRIMVSDSTRMAAAERIVTRPLDLIRVKGKTEPTAVHELVAKSGEASQKQLELIGV
                     FNRALDHYRGSRFAEALAEFQSALRIQPDDPPSLVYIDRCRHFLVSPPPAPWDGVFTM
                     TSK"
     gene            1557..2063
                     /locus_tag="PLD82_08470"
     CDS             1557..2063
                     /locus_tag="PLD82_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG65454.1"
                     /translation="MKRFSVVLAVLALTLTATALEAEQYKIQVRQTTLRATASAFGAR
                     VATLQFNTAVSILGQQGGFYQISSPYGNGYVTKSAVVPQSAFNSQYVAVQNTASYSQD
                     AATAATKGFSDAEVQESRKPGARYDLMRQAMEFSKINRDESWWAEFRRAGFLGENRRM
                     IQNKGYRK"
     gene            2060..>2449
                     /locus_tag="PLD82_08475"
     CDS             2060..>2449
                     /locus_tag="PLD82_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65455.1"
                     /translation="MKRTLIILTVVALVLAVAACGSLSNIVRTTDTIVQGADKIGKYQ
                     DAKNIHFSEADEFYIGRRVLANFFASYQPLFDRQLTDFVSRVGQTLAQVSQRPDVWEG
                     YRFIVYKSAGISAYCMPGGFILVSTGLL"
BASE COUNT          546 a          752 c          640 g          511 t
ORIGIN      
        1 gacctgacac tggccggcat gaagcataca ctcgacatcc gggaaatccg ggtctcccat
       61 gggcggctgg atctcgtcgg acccggtgga atcctccgca ccatccccct cgacgactcc
      121 ggcaacctca gcctgaaatt ttacggaccg ttccgcaagc tctacaacgc acacaagcac
      181 aatttctttt tggcctccga tatcatcaat gcctcgatag aaccagagtc tttcaccaat
      241 gtccggccgg aatacttcaa aaacaagatc gtctttttgg gaacggatgc tccggggctc
      301 aaggacatcc ggcccaatcc tttcagcaat acggacggag gggtctacct ctactccacc
      361 gcaatggaaa acatcctctc ctccgattac atcaccgaag catacgacga gtggctgaca
      421 atcctgctca tcctcctcct ctcgatgatc acggggtacg tcggagcccg cctgagcgtg
      481 atccgtggag ttctggcctc actgggcatg atactggtct attccttcgt cgccctgctg
      541 ctcttctcga gcgcctcgat ccttatcgag atggcaagtg tgttgacagc caccctcctg
      601 agctttttgg tgggcgctgt gcggaactac tatctcgaga gccggcaaaa acacttcatc
      661 cagggagcat tctcccaggt tctggcaccg gccattctgg aaaaactgat cgaggatccc
      721 agctccctga cactcggggg cgacagcaag gaactgacca tcttcttctc ggatctggcc
      781 ggattcacga cactctccga agccctcggg agtccacagc ggctcgtgtc aatcctcaac
      841 gagtacctga cggcgatggc cgacatcatc atggactacg acggatacgt ggacaaatac
      901 gaaggggatg ccatcatggc cttctggggc gcaccggtcg atgacccgaa ccatgcctgg
      961 aagggatgct acgccgcgat cgacaaccag atcaaactgg cagagctcca ggaacgcttc
     1021 gtttcgatgg gacttcccgg aggactcgcc gtccggatcg ggctgaacac cggtgttgct
     1081 gtcgccggaa tgatgggttc catgaaaaag ctgaactaca cgatcatcgg ggatcctgtc
     1141 aacctggcat cccgacttga aggtgccaac aagcagtacg gaacacggat catggtctca
     1201 gactcgacac ggatggcggc cgcggagcgg attgtcaccc gcccgcttga cctgatccgc
     1261 gtcaaaggga aaaccgagcc gaccgcagtc cacgaacttg tcgccaaatc cggtgaagca
     1321 agccagaaac aactcgaact gatcggtgtc ttcaaccggg cacttgatca ttaccgggga
     1381 agccgcttcg ccgaggctct ggccgaattc caatcggcac tcagaattca gcctgacgat
     1441 ccgccctctt tggtatatat tgacaggtgc aggcatttcc ttgtcagtcc gccgcccgca
     1501 ccgtgggacg gtgtattcac aatgacctcc aaataaggtc aagagaggta tcacacatga
     1561 agcgtttttc cgtcgtcctt gccgtcctgg cactcaccct gacggcaacg gcacttgagg
     1621 ccgagcagta caagatccag gttcgccaga ccacgctccg ggccacagcc agcgcctttg
     1681 gtgccagagt tgccacactc cagttcaata ccgctgtctc gatccttggg cagcagggcg
     1741 gattctatca gatttcctca ccgtatggaa acggctacgt aaccaaatcg gccgttgttc
     1801 cccaaagtgc cttcaacagc cagtatgtcg ccgtacaaaa cacggcctcc tactcccagg
     1861 atgccgcgac tgctgccacc aaaggcttct cggatgccga ggtccaggaa tcccgaaagc
     1921 cgggggcccg ctacgacctg atgcgtcagg cgatggaatt ttccaaaatc aaccgggatg
     1981 aatcctggtg ggccgaattt cgtcgtgccg gctttctcgg cgaaaaccga cggatgatcc
     2041 agaacaaggg gtatcgtaaa tgaagcgcac actcatcatc ctgacagttg tcgcccttgt
     2101 gctggccgtt gccgcctgcg gcagtctttc aaacatcgtc cggaccacgg acaccatcgt
     2161 ccagggggcc gataaaatcg gaaaatatca ggatgccaag aacatccatt tttccgaagc
     2221 ggacgagttt tacatcggcc gccgggtctt ggccaatttc tttgcgagtt atcagccgct
     2281 cttcgaccgt caattgacgg acttcgtctc ccgagtcggg cagaccctgg cccaggtcag
     2341 tcaacggccg gatgtctggg agggataccg ttttatcgtc tacaagagtg ccggcatctc
     2401 ggcgtactgc atgcccggcg gtttcattct ggtctcgaca ggactcctg
//
LOCUS       DAOSHE010000335         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_41936_length_2447_cov_4.364130, whole genome shotgun sequence.
ACCESSION   DAOSHE010000335 DAOSHE010000000
VERSION     DAOSHE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41936_length_2447_cov_4.364130"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLD82_08480"
     CDS             complement(<1..771)
                     /locus_tag="PLD82_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG65456.1"
                     /translation="MLGLSRKRGKTDRAQLAANREQLRQMLREKRFGLVESERTLLLN
                     VLDVHDTIVREIMVPRIDMVTVGVKEGPAGLIEKVKKFGHSRLPLIGEDQDTVLGIIY
                     AKDFIGRDSRSMKDKSLVKMGRQAIFVPETKRIFDLLEEFRQQRMHIAIVVDEYGGVS
                     GLVSLEDVLELFVGDILDEYDSEETPFVPSGRNTWNIDARMSVHDVNAETGADLPEDL
                     ADTLGGLFLELFGTVPRRRESVVCRGHRLRVHTLRENRI"
     gene            complement(737..1210)
                     /gene="ybeY"
                     /locus_tag="PLD82_08485"
     CDS             complement(737..1210)
                     /gene="ybeY"
                     /locus_tag="PLD82_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG65457.1"
                     /translation="MANTVQIRSDKGIHPVDKRLVRRLALAILKHLQAEDAELSVYLC
                     STETIHGLNRDWRGKDQPTDVLSFPQDCLDGPISGPLGDIVLSTDVAVAQAAREGVTY
                     ESELLRLLVHGALHLVGYDHETGRPEAVRMRTEEKRILGMMEAERAGIVTKTGKN"
     gene            complement(1232..2176)
                     /locus_tag="PLD82_08490"
     CDS             complement(1232..2176)
                     /locus_tag="PLD82_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG65458.1"
                     /translation="MVEQESIEIGLRVHDPYVLQERHQRLIEDQLGVVIRFDENGIRV
                     SGPGDRLDLAEKLLRFMHKPGNTVSDGELKYYIRLLVQQPDFELSALADETIRVSRQG
                     KMVRARGRGQLAYLAAIRSHDIVFGIGPAGTGKTYLAMAWAVSSLLAGQVSRIVLVRP
                     VVEAGENLGFLPGDLTEKINPYLRPLHDAMLEMLGRDEYAVFFEKGFVEVAPLAYMRG
                     RTLANAVIILDEAQNTTVTQMKMFLTRLGNDSKVIITGDATQTDLPSRVKSGLVHARR
                     ILEGIDGIAFVDFTAADVVRHRLVQEIINAYAGDGDGA"
     gene            complement(2228..>2447)
                     /locus_tag="PLD82_08495"
     CDS             complement(2228..>2447)
                     /locus_tag="PLD82_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65459.1"
                     /translation="LVLDKRMKMLDSFDIATPAGVTRVYSFFADYWIGRREDGHWENS
                     GKRDFFWNNSRPHPGTVYRIDFLGRQTS"
BASE COUNT          555 a          739 c          600 g          553 t
ORIGIN      
        1 gatcctgttc tcgcgaaggg tgtggacacg caatctgtgt ccacggcaaa cgacggattc
       61 gcgtctgcgg ggtacggttc caaacagttc gagaaaaaga ccgcccaggg tgtcggccag
      121 atcctccgga agatccgccc cggtttcggc attgacatca tgcacggaca ttctcgcgtc
      181 gatattccag gtattccggc cggatggaac aaatggcgtc tcttcactgt cgtactcatc
      241 caggatgtcc ccgacaaaga gttcgagaac atcctcaagc gaaaccagcc cggatacacc
      301 accatactcg tcaacaacaa tcgcgatatg catccgctgc tgcctgaact cctccagaag
      361 atcgaagatc cgcttggtct ctgggacaaa tatggcctgc cgtcccatct tgaccagtga
      421 tttgtctttc atcgaccggg aatcacgtcc gatgaagtcc ttggcgtata tgatacccag
      481 aaccgtatcc tgatcctctc cgatcaaggg gagtcttgaa tgcccgaatt tcttcacctt
      541 ttcgatcagt ccggccgggc cttccttcac gccgaccgtg accatatcga ttcgggggac
      601 catgatctcg cggacaatcg tgtcatgcac gtcaagaaca ttcaaaagca gggtccgttc
      661 gctttcgacc agcccgaaac gtttttcacg cagcatctgt ctcagttgtt cccggttggc
      721 cgcgagctgg gccctgtcag tttttccccg ttttcgtgac aatcccagca cgttccgcct
      781 ccatcattcc aagaatccgc ttttcttcgg tacgcatccg cacggcttcc gggcgaccgg
      841 tttcatggtc atagccgaca agatgcaagg caccatgaac cagaagccga aggagttcgc
      901 tctcgtatgt cacaccttca cgtgctgcct gtgcaaccgc gacatccgtt gaaagaacaa
      961 tatccccgag aggacccgat atcggtccgt ccagacaatc ctggggaaat gacaggacgt
     1021 ctgtcggctg gtcctttccc ctccagtcac gattgagacc atggatggtt tctgtcgaac
     1081 agagatagac cgacaactcg gcatcctctg cctggagatg tttaaggatt gccagagcca
     1141 gccgccgaac caagcgcttg tcaactggat gaatcccctt gtccgaacga atctgtaccg
     1201 tatttgccat gccacatccc tcccccggac cttatgcccc atcaccgtca ccggcatacg
     1261 cattgatgat ttcctgaacc aaacggtgac gcacaacatc cgccgcggta aaatcgacaa
     1321 atgcgatacc gtcgatacct tcaaggatcc ggcgcgcatg gaccaggccg ctcttgaccc
     1381 tggaaggaag atccgtctgt gttgcgtccc cggtaatgat cacttttgaa tcattcccca
     1441 atctcgtcaa gaacattttc atctgggtca cggttgtgtt ctgggcctca tcgaggatga
     1501 tgacggcatt ggcgagcgtt ctcccgcgca tataggcaag cggcgccact tcgacaaagc
     1561 ctttctcgaa aaaaacagcg tactcatccc gcccgagcat ctcgagcatg gcatcatgca
     1621 agggcctgag atagggattg atcttctctg tcaggtcacc cggaaggaac ccgaggtttt
     1681 ctccggcttc gacaaccgga cggacaagaa caatccgtga gacctgtccg gccaagagac
     1741 ttgacacagc ccaggccatg gccagatagg tcttgccggt accggccgga ccgattccga
     1801 agacaatgtc atgggaccgg atggcggcca agtaggccag ttgcccgcgt ccccgggccc
     1861 gtaccatttt tccctggcgt gaaacacgga tcgtctcatc ggccagcgcg ctcaactcga
     1921 aatccggctg ttgcacgaga agccggatat aatacttgag ttcgccgtcg ctgaccgtgt
     1981 ttcccggctt gtgcatgaac cgcaggagtt tttcggctaa atcaagtctg tcgccgggtc
     2041 cggagacccg tatcccgttt tcgtcaaaac ggatcacgac acccagctga tcctcgatca
     2101 atcgctgatg ccgttcctga aggacgtacg gatcatggac ccgaagcccg atctcgatac
     2161 tctcttgttc aaccaaagaa gtcttgatct tcgttctcca aatctttttg caggatttcc
     2221 ggatgactca ggatgtctgc cgtccaagaa aatcgatccg atagactgtt ccgggatggg
     2281 ggcgtgagtt gttccagaaa aaatcccgct tgcccgagtt ctcccagtga ccgtcctccc
     2341 tccgtccgat ccagtaatcc gcaaaaaatg aataaacccg cgtcacgccc gcaggtgtgg
     2401 caatatcgaa actatccagc attttcatgc gtttgtccag aaccaat
//
LOCUS       DAOSHE010000336         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_42143_length_2439_cov_2.604866, whole genome shotgun sequence.
ACCESSION   DAOSHE010000336 DAOSHE010000000
VERSION     DAOSHE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42143_length_2439_cov_2.604866"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLD82_08500"
     CDS             <1..385
                     /locus_tag="PLD82_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG65460.1"
                     /translation="MQEYARKNPKISFLMSHVIVDALGEDRLTGLRLRDLTTNEVKEV
                     EADGLFFAIGHDPNTGIFKPFLETDEAGYLKRQPDRTATTIPGVFAAGDVADNYYRQA
                     TTAAADGCRAALEVEKFLLSQGEAH"
     gene            516..1361
                     /locus_tag="PLD82_08505"
     CDS             516..1361
                     /locus_tag="PLD82_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOG65461.1"
                     /translation="MFAARFWLSTQCRPPRPRYGPRLVTSLLMLLATSLPLGAVALEQ
                     HEWNYFLAASDPVVFQYSTKIRVMTDGESHGQYAGSGSVLRAGLYLAYSQTTLWDLWA
                     WDESTPMIESSYRPEAFWRFVPETGPLAWIDAGGIHESNGLGGARIADSRAWNRLMIS
                     GGLPLAGRALVLVPRLWCILTDEGNGRIGKYAGYGELELALDLPLGGSRLVGSMRLRK
                     GWSSALDHGSIQINLAWQPWAAPEAGGFPFAFVVQAFSGYGDTMIAYDKKEHHMRAGL
                     AMFFD"
     gene            1573..1821
                     /locus_tag="PLD82_08510"
     CDS             1573..1821
                     /locus_tag="PLD82_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65462.1"
                     /translation="MRIVTIMVLTGVLLAGGLFLACSSSPEVKDSLSTEGRADLDEVP
                     MTRSGTTSSKTNVKVKTCPRCGQQNNTGKRCSFCDKKL"
     gene            2115..>2439
                     /locus_tag="PLD82_08515"
     CDS             2115..>2439
                     /locus_tag="PLD82_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65463.1"
                     /translation="MSDEKTSEYSPWWVLLENLLIIAMLVFGFLGMRFVAINGFPLLS
                     TLWAAFLLIMLGFVLRRTLCRHCKSFGKLCHCGWGRYARLVTTPGTPGTYPLPWLGGL
                     VWGIFM"
BASE COUNT          513 a          707 c          720 g          499 t
ORIGIN      
        1 catgcaggaa tatgcccgaa aaaatcccaa gatcagcttt ttgatgagcc acgtcatcgt
       61 ggacgcgctc ggagaagatc gcctgacggg gctcaggctg cgggatctta caaccaacga
      121 ggtcaaggag gtcgaggccg acggactctt tttcgcaatc gggcacgatc cgaacacggg
      181 aatcttcaag cccttccttg agacggacga ggccggctac ctcaagcgcc agcccgaccg
      241 gaccgcaacc accattcccg gagtgttcgc agcgggcgac gtggccgaca actattaccg
      301 tcaggcaacc acggcggcag ccgacggatg ccgggccgcc ctcgaggtcg aaaaattcct
      361 cctctcccag ggtgaagcgc attaagcccc acgaaaatcc gtgtttgcag tatgtttttg
      421 gggaacagtc ttcaggccat tcaggctggc ggctgttccc cgaaaacgct ttcagccttt
      481 tctatccgca caaccagcct gaacaaggag ccataatgtt cgcagcccga ttctggctct
      541 ccacgcagtg caggccccct cgcccacgat acggcccgag gttggtcacc agcctcctca
      601 tgctcttggc aaccagcctc cctctcggtg ccgtggcgct cgaacagcac gagtggaatt
      661 attttttggc cgcctcggat cctgtcgttt tccaatattc gacaaaaatc cgggtgatga
      721 cggatggcga aagccatgga cagtacgccg gaagcgggag tgtcctcagg gcaggactgt
      781 atctggccta ctcgcagacc acgctttggg acctctgggc ctgggacgaa tcgaccccga
      841 tgattgagtc cagttaccgg ccggaggcct tctggcgctt tgtaccggag acaggcccgc
      901 tggcctggat cgatgccgga ggcatccacg agtcaaacgg actgggcggg gcgcgcatcg
      961 ctgactcacg ggcgtggaac cggctgatga tcagcggagg gctcccgctg gccggacgcg
     1021 ccctggtact cgtgccacgg ctctggtgca tcctgaccga tgagggcaat ggccggattg
     1081 gaaaatacgc aggctacggc gaactcgaac tggcgctcga cctgcctctg ggcggctcac
     1141 gtcttgtggg aagcatgcgc ctgcgtaaag gttggagcag cgcactggat cacggaagca
     1201 tccagatcaa tctggcctgg caaccctggg ccgcacccga agccggaggc tttcccttcg
     1261 cgtttgtcgt ccaggccttt tcggggtacg gggatacgat gattgcctat gataaaaaag
     1321 agcaccacat gagagccggt ctggcgatgt tctttgactg acaggggctt caagacgggc
     1381 cgctttcagg ggaagcaacc acaaaaacgt gaaatcatga tgcgaatccg ggattcgcat
     1441 tgctggagta caaatccatc atgggtatcc cggactctcg cccccccata aaatccggca
     1501 tcagtgccag tcaaatggcg tctgtggggt atcatgtatg gtgagaagca gtatgtgaca
     1561 aggagggaat ctatgcgcat tgtgacaatc atggtgttga ccggagttct cctggccggg
     1621 ggactctttc tggcctgttc atcaagtccg gaggtcaagg attcgctgtc caccgaggga
     1681 agagcggatc tggatgaagt acccatgaca cgatccggga ccaccagttc gaaaaccaat
     1741 gtcaaggtga agacctgccc ccgctgtggt cagcaaaaca acacgggaaa acgctgctcc
     1801 ttttgcgata aaaaactgta agtccccaaa tcgatgcggg tgaggattgg tcccccggga
     1861 gggtgtcata ccgtctcact gcccgcccgg tggtttgcgt accgcaggac aagcgcgtag
     1921 tgggtgaaga ggcgcccgct gtcaccacag gcaaccagat tcgggatcaa ccaggagttc
     1981 ccgcgtatgg accctcgtgt gcatctgtgg ccccgccgta ccggtatcaa cacccggatt
     2041 ggcacaggaa tgctggacaa ggtctggccg gtagtgtatc ctgtcgtcaa tatttcagtg
     2101 tcgaaggagc cggcatgagc gacgaaaaaa cttctgagta ttccccctgg tgggttctcc
     2161 tcgagaatct tctgatcatt gccatgcttg tcttcggttt cctcgggatg cgctttgtcg
     2221 ccatcaacgg attccccctg ctgtcaacac tctgggcagc ctttctcctg atcatgctgg
     2281 gcttcgtgct gagacgcacc ctgtgcaggc attgcaagtc attcggaaaa ctctgccatt
     2341 gcggctgggg gcggtacgcg cgcctggtca ccacaccggg aactccggga acatacccgc
     2401 tgccctggct gggtggactg gtctggggca tctttatgg
//
LOCUS       DAOSHE010000337         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_42368_length_2430_cov_2.528842, whole genome shotgun sequence.
ACCESSION   DAOSHE010000337 DAOSHE010000000
VERSION     DAOSHE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42368_length_2430_cov_2.528842"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLD82_08520"
     CDS             <1..855
                     /locus_tag="PLD82_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG65464.1"
                     /translation="SHLKAIGFKNRPQLDAVLAMVDRAGIGFDHYPYPWGSTSLLSLI
                     PPHMMRDGVTHLLERLGDLSVQNELEGGWSGGRALEQGAPWDNLPALVGWKNIRIVHA
                     AHGAADDCRGFPVNEAAALRGCSPSALVCQVLRDARGDARMIDEYMEEEAVVQILRHP
                     AGMIASDSLFGPGAHPRVSGCFPRVFERYVFDRPVLRLEEAVRKMTGATAAWLGLEDR
                     GLLREGLVADLVVCTGDVAEPEPGSAPRGIRLVMINGEIVARDGKRVSGKPSGMILRA
                     NGKSDPQR"
     gene            complement(869..1126)
                     /locus_tag="PLD82_08525"
     CDS             complement(869..1126)
                     /locus_tag="PLD82_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65465.1"
                     /translation="MHTLRTILSIFFILLASSLVAQSTNSAAQKDAKAELQAKISRAH
                     EMVTLYMYLYSRDTGTERHYRAYQYWFKQRQELVKQLASHP"
     gene            1539..>2430
                     /locus_tag="PLD82_08530"
     CDS             1539..>2430
                     /locus_tag="PLD82_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65466.1"
                     /translation="MKTLKVLFAVLLAVSVLAGCSLAPDTVGVTEDSVQRTVTTYTET
                     FANCTKTGTSYVSGSFVGVGGWTWNYVSAAWSGGDINGKTPVLGKGKSPAASVYTTVN
                     NGVGTISFKVKKPYSTAVNVTLKMNGTTIATITHSSTTAATKGPYTINRTGSVKIEIV
                     QTSTSAGQASIDDITWTSYDGGTSSSSSSSSVSSSSSSFSSSSSSVAAGWVRETSTVA
                     SPNYPSAYGNNANVTSTFSKTGATKVRVHFSAFNTENNYDKVYLKTSSGSTVATYTGN
                     KGAFLSAEVTGSSALVNFTSD"
BASE COUNT          527 a          664 c          703 g          536 t
ORIGIN      
        1 tcccatctca aggcgatcgg tttcaaaaac cggccccagc ttgatgccgt gcttgcgatg
       61 gtcgacagag ccggaatcgg gtttgaccac tatccttatc catgggggag taccagtctg
      121 ctctcgctga ttcctccgca catgatgcgg gacggggtga cgcatcttct cgagcggttg
      181 ggggatcttt ccgtgcagaa cgaactggag gggggctggt cgggagggcg cgccctggag
      241 caaggcgccc cctgggacaa tctcccggca ctggttggat ggaaaaatat caggatagtg
      301 catgccgccc atggcgcggc cgatgattgt cgcgggttcc cggtcaatga agcggcagca
      361 ctgagagggt gcagcccgtc cgccctcgtt tgtcaggtgc ttcgcgatgc ccgtggtgat
      421 gcacggatga tcgatgaata tatggaagaa gaagctgtcg tccagattct tcgccatccg
      481 gcgggcatga ttgccagcga ttccctcttc ggtcccggcg cccatcccag ggtctctggc
      541 tgttttcctc gtgttttcga gcgatatgtg tttgatcggc cggtcctcag gcttgaggag
      601 gcagtgcgca agatgactgg cgcgaccgcc gcttggctcg gtcttgagga tcgggggctg
      661 ctccgggaag ggttggtggc cgacttggtt gtctgtacgg gagatgtggc ggaacccgaa
      721 cccggaagtg cgccccgggg aatccgcctg gtgatgatca acggggagat cgtggctcgc
      781 gacgggaagc gtgtctcggg aaaaccgtcc ggcatgattc tccgtgcaaa tggaaagagc
      841 gacccgcagc gatgagcggg ttgttttttt acgggtggct ggcgagctgt ttgaccagct
      901 cctgcctttg tttgaaccag tactggtagg cccgatagtg acgttctgtt ccggtatcac
      961 gggagtagag gtacatatag agggtcacca tctcgtgggc ccggctgatc ttcgcctgga
     1021 gttctgcctt ggcgtctttt tgtgctgcac tattggttga ctgggcaacc aggctgctgg
     1081 cgagcaggat gaaaaaaatg gaaagaatcg tgcgtagggt atgcatatcg gctgactcct
     1141 cgtcgcggac tgaagctggg actgcttcta ttttatgcag gtcgggctgc gctgtcaagg
     1201 agtaccgata cgcgcgcgtg tcaacgcgcg aagcagagtg atgccgggcg gacctgtcat
     1261 tcctgagagg ccatatctga tcgatctatc gtgtttttgt gatccgggaa acacatgata
     1321 aaaacgcaaa attatgcctg gacctccgga gtcatatgac aagaccataa aaaaccgagt
     1381 cggcaacgtc gaaaaatcca gaggaagatt gtcaagaaga taacaaaaca aaaatatgct
     1441 aaaaaattca ttttttgttt gacaggaatt cgcggtgggg gcatcatgtc cccgaagggc
     1501 gattaacgcc tcacttaaca tctccaagga gccaaggtat gaaaacgttg aaagtgcttt
     1561 ttgccgtttt gctcgcggtt tccgttcttg caggctgtag cctcgcaccg gatactgtcg
     1621 gtgttaccga agacagtgtg cagcgcactg tcaccaccta caccgagact tttgccaact
     1681 gtacgaagac cgggaccagc tatgtatccg gcagtttcgt cggtgtcggc ggatggacct
     1741 ggaattacgt ctctgccgca tggagcggtg gcgacatcaa cgggaaaacc cccgttctcg
     1801 gcaagggcaa gagcccggca gcatcggtct acaccacagt caacaacggt gtcggaacga
     1861 tctcgttcaa ggtgaaaaaa ccgtattcca cagcggtcaa tgttaccctc aagatgaatg
     1921 gcacaacgat cgccaccatc acccacagca gcacaacggc tgcaacaaaa ggtccctaca
     1981 ccatcaaccg cacaggatcc gtcaagatcg agatcgtcca gacctcgacc tcggccggtc
     2041 aggcctcgat tgacgacatc acctggactt catacgacgg cgggacaagc tcttcgagtt
     2101 cctcgagctc ggtttcctcc tcttcgtcct ccttttcaag ctcgagctcc tcggtcgcgg
     2161 ccggatgggt gcgcgagacc agcacagtcg cttcaccaaa ctacccctca gcctacggca
     2221 acaatgccaa cgtgaccagc accttctcca agacaggcgc aaccaaggtt cgtgtgcact
     2281 tctcggcctt caacaccgag aacaactacg acaaggtcta tctgaagact tcttccggct
     2341 cgacggttgc cacctatacc gggaacaagg gagccttcct ttccgccgag gtcacaggtt
     2401 cgtctgcgct tgtcaacttc acctctgatt
//
LOCUS       DAOSHE010000338         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_42474_length_2426_cov_2.504850, whole genome shotgun sequence.
ACCESSION   DAOSHE010000338 DAOSHE010000000
VERSION     DAOSHE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42474_length_2426_cov_2.504850"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..435
                     /locus_tag="PLD82_08535"
     CDS             106..435
                     /locus_tag="PLD82_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019098375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG65467.1"
                     /translation="MIPSLLHLLEDLPDAQSAEVIEILAESPGVRIERIVSGGQASPP
                     GFWYDQDEHEWVMLLSGTASLRWEDGTVTVLETGEALEIPPHLKHRVENTTDQTVWLA
                     VFWSQPI"
     gene            complement(428..1639)
                     /locus_tag="PLD82_08540"
     CDS             complement(428..1639)
                     /locus_tag="PLD82_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65468.1"
                     /translation="MGNPAYRPKFHIHWIVSFLGAIGCFLVMFMIDALATTVALLVIL
                     LLYGWYARRNLQTNWGDIRKGFWASVIETGMVNYHKYDLHPRNFRPHIAILEGQRRGR
                     KILVDTADLLVGKHGIISDYVFLTEKNQQTPEAAADEFGRMRQFLDENRTPYIFPEII
                     RSRNMEQAQLLVLQADGVGTFKANTILADIYCDDDSIEEQLRLVPDYLGLGKNVLLVK
                     AAANEQVAQSRIDIWLSGFTSNLSIMLLIPYLVTRNPDWTGTRICLRMIVHTEDERQR
                     AQKNFQAILAHARIPAEAEILALHDGHGLGQGSVREGVPTGKGKKNVFSRIMDFIRDF
                     AVESYTDDEHARINGIIAERSADASMVVLGMRIPEKGKEKRYAREIKTLIEKLPMTIL
                     VKGQDMVNLFK"
     gene            complement(1676..>2426)
                     /locus_tag="PLD82_08545"
     CDS             complement(1676..>2426)
                     /locus_tag="PLD82_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG65469.1"
                     /translation="AQAFSIAFYIIGFTKALALFEPFSAIDPVALNMATLAILTVLSI
                     FSANIAIRAQYLIFLLLFAAIGAFVLGNTAFDTSPQWFGDFTQGDFWQSFAIFFPAAT
                     GIFAGLSLSGDLKEPHRNLPRGTIASVIVTGVVYLVLAAIFSTNHAPAVMLANESLMQ
                     TSAAIPELVIAGVFAATISSAIGMLLAAPRTLQALALDGMAPGVFARGRGPANEPRLA
                     MLVSVLLAATLLGAGSIDFVSQILTMFFLTS"
BASE COUNT          596 a          667 c          638 g          525 t
ORIGIN      
        1 ggcggacctg gtagccccga cctttctggc cagcgagctc gtctaccgga tgatggggat
       61 tgccatgcgg agcgccccgg gcgggcggac atgcggagcc ggaagatgat tccgtctctc
      121 ctgcacctcc ttgaggatct tccggacgca caaagcgccg aggtcatcga gatcctggcg
      181 gaatctcccg gagtccggat cgagcggatc gtttctggag gacaggcctc ccctcccgga
      241 ttctggtatg accaggacga gcatgaatgg gtcatgctgc tctccgggac tgcctcgctt
      301 cgctgggaag acgggacggt aaccgtcctg gaaaccggcg aggccctcga gatcccgccg
      361 catctcaaac accgggttga aaacacaaca gaccagacgg tctggctcgc tgtgttctgg
      421 agccagccta tttgaagaga ttgaccatat cctgtccctt gacgagaatg gtcatcggaa
      481 gcttttcgat cagtgttttg atttccctgg catagcgctt ctcttttccc ttttcaggaa
      541 tcctcatgcc aagcacaacc atgctggcat cagccgaacg ttcggctata atcccgttga
      601 tccgggcatg ttcatcatcc gtatatgact cgacggcaaa atccctgata aaatccatga
      661 tccgggaaaa cacatttttt ttgcctttac cagtcggtac gccctccctg acactcccct
      721 gtccaaggcc atgcccgtca tgcaacgcca gtatctcggc ctctgccgga atcctggcat
      781 gagccaggat ggcctgaaaa ttcttttggg ccctctgccg ttcatcctcg gtatggacaa
      841 tcatccgcaa gcagatcctg gtcccggtcc agtcaggatt gcgtgtcacc agatagggaa
      901 tcaggagcat gatggagaga ttggacgtaa accccgaaag ccagatgtcg atccggctct
      961 gtgccacctg ctcgttggct gctgccttga caaggaggac attttttcca agaccgagat
     1021 agtccggtac aagacggagc tgttcctcaa tcgaatcatc atcgcaatag atatccgcca
     1081 ggatcgtgtt ggccttgaag gtcccgacac cgtcagcctg gaggacaagg agttgcgcct
     1141 gttccatgtt tctcgatcga atgatctcgg gaaaaatata tggtgtcctg ttttcatcga
     1201 gaaactgccg catccggccg aattcatcgg cggccgcctc gggagtctgc tggttttttt
     1261 ctgtcaaaaa cacatagtcc gagattatcc cgtgctttcc gactaaaaga tcagcagtgt
     1321 ccaccaggat cttgcgcccc ctccgctgtc cttcgaggat ggcaatatgt ggtctgaaat
     1381 tcctgggatg caggtcatat ttatggtaat tgaccatgcc tgtttcaatg actgatgccc
     1441 aaaacccttt ccggatatcc ccccagtttg tttgaagatt tctccgggca taccagccat
     1501 agagcaggag aatgaccagc agggcaaccg ttgttgccag tgcatcgatc atgaacatga
     1561 ccaaaaaaca gccgatcgcc cccaaaaaag aaacaatcca gtggatatgg aacttcggtc
     1621 tgtaggccgg attacccacc agtgcctcaa gggcggcaac aagattgaca cttccctaac
     1681 tggttaaaaa aaacatcgtc aggatctgtg aaacaaagtc gatactgcct gcccccagca
     1741 gggtagccgc caggagaacg ctgaccagca tggccagacg tggctcattg gccggacctc
     1801 ttcccctggc aaacaccccg ggtgccatcc cgtccaaagc aagcgcctgc agcgttcggg
     1861 gagcggccaa aagcatcccg atggcggacg atatagtggc cgcaaacaca ccggcgatca
     1921 cgagttcggg aatggcggca ctcgtctgca tgagtgactc gttggcaagc ataaccgctg
     1981 gagcatgatt ggttgaaaag atggcagcca acacaaggta gaccactccg gtgacgatta
     2041 ccgaagcgat ggtcccccgt ggcagatttc tgtgaggttc tttgagatcc ccggaaagcg
     2101 agagaccggc aaaaataccg gttgccgcag ggaagaaaat ggcgaaactc tgccaaaaat
     2161 caccctgtgt gaaatcgcca aaccattgtg gactcgtatc gaaggctgtg ttccctaaaa
     2221 caaacgcacc tattgccgca aataaaagaa ggaagatcag gtattgcgcc cggatggcaa
     2281 tatttgcact gaatatggaa agaacggtca atatggcaag ggtcgccata ttcagggcaa
     2341 caggatcgat ggctgaaaac ggctcgaaca gggcaagtgc ttttgtaaag ccgataatat
     2401 aaaacgcgat tgaaaaggct tgcgca
//
LOCUS       DAOSHE010000339         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_42845_length_2412_cov_2.680526, whole genome shotgun sequence.
ACCESSION   DAOSHE010000339 DAOSHE010000000
VERSION     DAOSHE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42845_length_2412_cov_2.680526"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLD82_08550"
     CDS             <1..300
                     /locus_tag="PLD82_08550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HOG65470.1"
                     /translation="VILETCLLSDQEIVAACQLVMEAGADFVKTSTGFSSGGATSHAV
                     GLMRKTVGHNFGVKASGDVRSREDAEAIIRAGANRIGTSSGVAIVTGDEVIKGDY"
     gene            complement(425..1177)
                     /gene="tpiA"
                     /locus_tag="PLD82_08555"
     CDS             complement(425..1177)
                     /gene="tpiA"
                     /locus_tag="PLD82_08555"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG65471.1"
                     /translation="MRTPMIAGNWKMNMTVPQAEELARAIQAGFRGESGRDVLVCPPA
                     VCLETVRRIIGTARIRLGAQTVHPEGSGAYTGEVALDMLRSAGCDHVIIGHSERRAIF
                     GETDTQVARRMRAVLNGRLVPVLCVGETLAERKAGRAEEVVARQVSAAYAGVGTSDAL
                     VSVIAYEPVWAIGTGETATPEQADAMHASIRRILGKLYGEQVADSVRILYGGSVNEKN
                     IDELMRRPNIDGALVGGASLKPESFLRIVHYQ"
     gene            1537..2412
                     /gene="speA"
                     /locus_tag="PLD82_08560"
                     /pseudo
     CDS             1537..2412
                     /gene="speA"
                     /locus_tag="PLD82_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815358.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
BASE COUNT          464 a          784 c          704 g          460 t
ORIGIN      
        1 gtgatcctcg agacctgcct cctctccgac caggagatcg ttgccgcctg ccagctcgtg
       61 atggaagccg gtgccgattt tgtcaaaaca tccaccggat tctcttccgg cggagccacc
      121 agccacgccg tcggtctgat gcggaaaaca gtcggacaca acttcggggt caaggcctcc
      181 ggagacgtcc gcagccgtga ggacgccgag gcgatcatcc gggccggcgc gaaccggatc
      241 ggaacctcgt caggggtggc cattgtcacc ggtgacgaag tcatcaaggg cgattattga
      301 tttttgccga acatcctcgg gccgacagca tccggcccgt cctgccgggg tgcgttttcc
      361 agggagacgc cccccgtttt ttttgccgtt ccaacccgaa tcgggtcggg cgtagggtat
      421 tccgttattg gtagtggacg atgcggagga aggactccgg cttgagactc gccccgccca
      481 caagggcacc gtcgatattc ggccgtcgca tcaattcatc gatgtttttc tcgttgaccg
      541 atcccccgta gaggatgcgg accgaatccg ccacctgttc gccgtagagt tttcccaaga
      601 tcctgcggat ggaggcgtgc atcgcgtcgg cctgctccgg ggtagccgtc tctcccgttc
      661 cgatcgccca gaccggctcg taggcgatca cggagaccag ggcatcggat gtcccgacgc
      721 cggcataggc cgccgagacc tgtcgtgcca ccacctcctc cgctcgcccc gccttgcgct
      781 cggccagggt ttcaccgaca cagaggaccg ggacgagccg gccgttcagg acggcccgca
      841 tccggcgggc cacctgagta tccgtctccc cgaagatggc gcgccgttcg ctgtgcccga
      901 tgatcacatg gtcacaaccc gccgagcgga gcatgtccag agcaacctct cccgtatagg
      961 cgccggatcc ttcaggatgg acagtctggg caccgaggcg aatcctcgcg gtcccgatga
     1021 tccggcgcac cgtctcgaga cagacggccg ggggacagac caggacgtcc cgtccgctct
     1081 ccccccgaaa cccggcctgg atggcccggg ccagttcctc ggcttggggg accgtcatgt
     1141 tcatcttcca gttgcctgct atcatgggtg tccgcatgag gtcctccgaa tggttgtgcc
     1201 ctgttccctc aaagtactgc ggagacttcc tgacggcaat cgggctgaga cccggagcaa
     1261 cgcccggtct gcggatcttg gtcgggacgc ccgaatcaac gcaccctccc gggatatgac
     1321 gcaatccacc cacgaatccg gtttcaggaa aatcctagcc caggtctccc ggcaaccgca
     1381 agcctgtcgt tgcgagcgcg aacagggtct atacctctga ctgtgagtat gttatactcc
     1441 gagttgcggt ctccatcccc gtcgcagtcg ggaacaccac gcggtacccg atccgacctt
     1501 gtgtgtgcag acccatgaaa ccttggaggt acgcctttgg ccaagcagga catccttgga
     1561 cgctggtcca tcaatgattc cgcaaatctg tacggcatca aggattgggg agccggctat
     1621 ttcgatctct cccaaagtgg agaggttgtc atccgggcct gtcccggtga caagagttcc
     1681 gaagtcagcc tgatggaaat catctccgga ataaacgaac gcggaatggg aatgcccgtc
     1741 ctgctgcgga tagaaaacct cctggacagc cagatcagcc ggctgaacga gagtttccgc
     1801 aaggccatgc gcaaccttgg ataccgcgga cgctatcagg gcgtctttcc catcaaggtc
     1861 aaccagcagg cacaggtgat cgaggagatc tcgcgttttg gcggccggtt caaccacgga
     1921 ctcgaagccg gaagcaaggc cgagctggtg atcgcccttt cgatgctcca ttcgcgcgac
     1981 tgcctcctga tctgcaacgg ctacaaagac gaggaattca tccgcctcgg gctacacgcc
     2041 gtcaaactcg gctacaaatg cttttttgtc atcgagactc ccgccgaact ggagatcatc
     2101 ctccaggaga gcgcggccct cggggtccgc cccctgatcg ggatccgcct caagctctcg
     2161 acacgggcgg gcggccactg gcaggattcc ggcggggaca attcgatctt cggtctctcc
     2221 accaaccaga tggtggatgt cctcgaccgg ataaaatccg ccggtctgat cgactgcctc
     2281 cagctcctgc actaccatct cgggagccag atcccgaaca tccgcgacat ccgggccgcg
     2341 atccgcgaaa cagcacgcta ttatgccgag atggaaggaa ggcgccccga tgggctacat
     2401 cgatctcggt gg
//
LOCUS       DAOSHE010000340         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43106_length_2403_cov_2.402044, whole genome shotgun sequence.
ACCESSION   DAOSHE010000340 DAOSHE010000000
VERSION     DAOSHE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43106_length_2403_cov_2.402044"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLD82_08565"
     CDS             complement(<1..886)
                     /locus_tag="PLD82_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG65472.1"
                     /translation="MALKTIYLGLALLSISASLSASGFSTDQEAEVRRAVHLMDRKLP
                     HPERRILLAVNIPASRLIAHTGGQKAWRNRFSPGSLAKIPLVWHLMTEGRLSQDYTWR
                     CTRKFFPGGEGTGCDELIHEDIEYPARGQYYKCSLLKGHGVVSLEQALSVSCNHAFLN
                     LHDRLSPDKWDRFCRSSGLLGRNSLLELDPQAEPGMLDAPCCPRDRLLLPVGFGLHVT
                     PGAMAVFFTSLFSGGSVRTIVPAQSSRSGTPIGTLPLPTAVRNTILGGMRKAAHHGTA
                     RSLAIPGASILAKTGSGLI"
     gene            complement(877..1626)
                     /locus_tag="PLD82_08570"
     CDS             complement(877..1626)
                     /locus_tag="PLD82_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65473.1"
                     /translation="MQQSISAKSPGLTIFNTQRWPILLAGLLLLGLANTVHAKTELAP
                     GLSHAEASLCQSAVVLAQARMGQIFPWLRARSMPRVRFFPAGAPEGSASPWVGGIYHA
                     NDHRIDIQHVPRLISQGRLEWTVQHEFVHAALALSGTARLPLWLEEGSAIYLSAQTLD
                     ETDRSITPARSIAQLESGFRTHSISRNQSAWIHLKMYNRQARAIIAALVERNGIEHFT
                     GFLRTLKNGTPFSQALASWYGLAPSELSAWL"
     gene            complement(1604..2233)
                     /locus_tag="PLD82_08575"
     CDS             complement(1604..2233)
                     /locus_tag="PLD82_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65474.1"
                     /translation="MPEENRNALEAFLKALPGGFGLVFDGFTGNDNGTWPAARLVWNA
                     GPQAQDAMQKLETFVGSLPEAKIENGSHKDVPYRKLVGGVTQRFDPCYTRIGNLIVLS
                     LDEPSMKSAIDLAKGAQKVLADSSVLTTSLGKLRRESITHFSHIDVMRLVQNLYKEMG
                     EHAFRSKDFSRREVSSALGPLASEGPGFSSLTIVAGRDQGYTRATINIR"
BASE COUNT          500 a          607 c          783 g          513 t
ORIGIN      
        1 caatcaggcc gctgccggtc ttggccagga tacttgcgcc gggaatggcc agcgagcgtg
       61 ctgtcccatg gtgcgcagcc ttgcgcatcc ccccgaggat ggtgttccgg acagcggtcg
      121 ggagcggcag ggtgccgatc ggggttccgg accggctact ttgggctggc actatggtgc
      181 gaacgcttcc accggaaaaa agactggtaa aaaagacggc catggcccca ggtgtgacat
      241 ggagaccgaa cccgacaggg agcaggaggc ggtcccgggg acagcacggt gcgtccagca
      301 tccccggttc ggcctggggg tccagctcga gcagggaatt tcggcccaag aggccgcttg
      361 accggcaaaa ccggtcccat ttgtccgggg agagccggtc atggagattg aggaaggcat
      421 gattgcagga gacactcagg gcctgctcca atgaaacgac tccgtgtcct ttgagcaggc
      481 tgcatttgta atactggccg cgggcagggt attcgatgtc ttcgtgaatc aattcatcgc
      541 atccggtgcc ttctcccccg gggaagaatt tccgggtaca gcgccaggta tagtcctgtg
      601 acaaccggcc ctctgtcatc aggtgccaga cgaggggaat cttggcaaga gagcccggtg
      661 agaagcggtt tctccaggcc ttctgcccgc cggtatgggc aatcaaccgg gaggcgggaa
      721 tatttacggc taaaaggatg cggcgctccg gatgcggaag ttttcgatcc atcagatgga
      781 cggcgcgccg tacttcggcc tcctggtctg tcgaaaaccc tgacgcggag aggcttgccg
      841 aaatggaaag aagagcaagc ccaaggtata tggtcttcag agccatgcgc tcaactcgga
      901 gggtgcgaga ccgtaccatg aggcgagtgc ctgggaaaat ggcgtcccgt ttttcagtgt
      961 ccggagaaaa ccggtgaagt gttcgattcc gttgcgttca accagggcgg caatgattgc
     1021 ccgggcttgg cggttataca ttttgaggtg gatccatgcg gattgattgc gggagatgct
     1081 gtgggttcga aacccggatt caagctgtgc gatcgaccgg gctggagtga tggagcggtc
     1141 agtttcgtcg agtgtttgtg ccgagagata gatcgcacta ccttcttcaa gccagagggg
     1201 cagacgtgct gttcctgaaa gcgcgagtgc tgcatggaca aattcatgct gcactgtcca
     1261 ttccagacgg ccctggctga tcaggcgcgg gacgtgctgg atatcgatac ggtggtcgtt
     1321 ggcgtgatag atccctccaa cccaggggga tgcgcttccc tcaggggcgc ccgcaggaaa
     1381 gaaacggact cgtggcatac tacgagcgcg gagccaggga aaaatctgcc ccatgcgggc
     1441 ttgtgccaga acgacagcgc tttggcagag gctcgcttcc gcatgggaga gtccgggtgc
     1501 gagctctgtt ttggcatgaa cggtgtttgc caggcccaat aagagcaggc cagccaaaag
     1561 gatgggccaa cgttgtgtgt tgaatatcgt cagcccgggg cttttagcgg atattgattg
     1621 ttgcacgggt atatccctgg tcccgtccgg caacaatggt cagactggaa aaaccgggac
     1681 cttcggatgc cagtggacca agggcgctgg acacttcgcg gcgtgagaaa tctttactgc
     1741 ggaaggcgtg ttcgcccatt tctttgtaga ggttttgcac gagtctcatg acatcgatat
     1801 gagagaagtg cgtaatgctt tcgcgacgca gttttccgag agatgtcgtc aaaaccgaac
     1861 tgtcagcaag gaccttttgg gctccctttg ccaagtcaat agcgctcttc atggaaggtt
     1921 catcaagtga gagtacgatc agattgccga tgcgggtata gcagggatca aagcgttgtg
     1981 tgacgccacc gacgagtttg cgatacggga cgtctttgtg gcttccgttt tcgattttgg
     2041 cttcggggag tgagccgaca aaggtctcga gtttttgcat ggcatcttga gcttgcggtc
     2101 ctgcgttcca gaccaggcgg gctgccggcc atgttccgtt gtcgttgccc gtaaaaccgt
     2161 caaaaacaag accgaatcca ccggggagcg ccttgaggaa ggcttcgagt gcgttgcggt
     2221 tttcttcagg catcgattgg aagagatcgg cgagcagggg cgagagacgc catgtgtcgc
     2281 ctccggcaaa gcctgaatcc ccaaggacga gatgggtgtc agccggaagt tgtcccagga
     2341 tggcggtttc aagaacaggg tcgttggtgt tgctttatgg ggtccaactc gcgtcaatcc
     2401 gtg
//
LOCUS       DAOSHE010000341         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43232_length_2398_cov_5.352113, whole genome shotgun sequence.
ACCESSION   DAOSHE010000341 DAOSHE010000000
VERSION     DAOSHE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43232_length_2398_cov_5.352113"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLD82_08580"
     CDS             complement(<1..803)
                     /locus_tag="PLD82_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65475.1"
                     /translation="MRFFPALLGFLVILTGCGATSYTLGGGAGGSSVWRQMSAGMLEG
                     TSTGELALAADAGVVWLAWPDSAQEGKGVLWRHDASGWNLVSGKPFSTAAVSSIALLP
                     DGTNLGWICADESDAGRGRTMHTTGTNIETPSGGLVTSGRMASASVASYGGFPLAFVQ
                     DGGAAILKSFAGFSSWVIQPGGSSGIAVSPTLAARGNRYGLAWADGETGYKLSTAWYE
                     SGLFKAGGSGYSAGPVESLTSIMTASTWCVAGQAKAQNVLVAWTFDFGM"
     gene            1131..>2398
                     /locus_tag="PLD82_08585"
     CDS             1131..>2398
                     /locus_tag="PLD82_08585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG65476.1"
                     /translation="MNNRRFPIKRIGPDSKRRIIITISLLLATGFIITSLASYFVARS
                     SIRQNIVDNELPLTSDNIYSEIQKDLVRPTFISSMMSRDTFLRNWVLQGEVDTSRITQ
                     YLREIKEHYKTLTSFFVSEQTRTYYQANGVLKKVVEGEPRDAWYFRVRTMQTPYEINV
                     DPDMANRDTLTIFINFRVFDFATNYIGAAGVGLTVDSVRNLVNEYQKRYQRRVSFVDR
                     NGQIMVSGTNFRYEGKNIREVPGLDRIARTALASAETALQYEDRGETHLLNVRFLPEL
                     GWYLFVERTDIEAISAIRSALFLNIVLCIAVTAIVALLVSLTVGRYQKRLETMASTDF
                     LTGFVNRHALALLFTQLMKDLKRVPQPISLMMVDIDHFKRVNDLFGHLVGDCVIQKTA
                     AHIRSALRDSDITSRWGGEEFLVVLQRCTL"
BASE COUNT          522 a          768 c          630 g          478 t
ORIGIN      
        1 tgcatcccga agtcgaaggt ccaggcgaca agcacattct gtgccttggc ctgtccggcc
       61 acgcaccagg tggaggctgt catgatggat gtgagactct ccacgggccc ggcgctgtac
      121 ccggatcctc cggccttgaa gagaccggac tcgtaccagg ccgtcgagag cttgtacccg
      181 gtctctccgt cggcccaagc caaaccgtaa cgattccctc gagccgccag ggttggagag
      241 acggcgatcc cggacgagcc gccgggttgg atcacccagg aggagaaccc ggcaaaactc
      301 ttgagaatgg ccgcaccgcc gtcttggacg aaggccaggg gaaatccgcc atagctggcc
      361 actgaggccg acgccatcct gccgcttgtc accagtccgc ccgaaggtgt ttcaatattt
      421 gttccggttg tgtgcatggt cctgccgcgc ccggcgtccg actcatctgc acagatccag
      481 ccaagattgg ttccgtccgg caggagggca atggatgaca cggcggccgt cgaaaacggt
      541 tttccggaaa caagattcca accggaggca tcgtgccgcc agagaacacc cttgccctcc
      601 tgtgcgctgt ccggccaggc cagccataca acccccgcat cggcggcgag cgccagttcg
      661 ccagtgcttg tcccctcgag catcccggct gacatctgtc gccagacgct gctgccaccg
      721 gctccaccac ctaaggtgta actcgttgca ccgcaacctg tcaaaatgac aaggaatccc
      781 agcaaggccg gaaagaatcg cataacttac ctccgaggag aacacacact gcactctcaa
      841 aacactcaat cgtatcatac agcatatctc ccgcaatgac acgtttccgc gacagtactc
      901 tcggagtatc gttcccggca acgcatgacg ttttcacgaa gttgtgttgc gactccggga
      961 atcgcatgac tgtattgcaa atttgcgttt cgtagacgcg atcaatttcg gccctacgaa
     1021 agaaacccac cttgtttccg gccggcgcgt tgacaccgcg cgtacaggat tgacgcaggc
     1081 gaaaccgcat tgtacactga cacttcttgt cagcctgaac acggttcatc atgaacaacc
     1141 ggaggtttcc catcaaacga atcggccctg acagcaaacg ccggatcatc atcaccatca
     1201 gtctcctcct ggcgaccgga ttcatcatca cctcgctcgc cagttacttt gtggcccgaa
     1261 gcagcatccg gcaaaacatc gtcgacaacg agctcccgct gacatcggac aacatctact
     1321 ccgagatcca gaaggatctg gtccggccga cctttatctc ttcgatgatg tcccgtgaca
     1381 cgtttttgcg gaactgggtc ctccagggcg aggtggacac atcccgcatc acccagtacc
     1441 tgcgggagat caaggaacac tacaagacct tgacgagttt ttttgtctcg gagcaaaccc
     1501 gcacctacta ccaggccaac ggcgtcctca aaaaagtcgt cgaaggggaa ccacgcgacg
     1561 cttggtactt ccgtgtccgc accatgcaga cgccctacga aatcaatgtg gatcccgaca
     1621 tggcgaacag ggatacactg acgatcttta tcaacttccg tgtttttgac tttgccacaa
     1681 actatatcgg tgccgcggga gtcggcctga ccgttgattc ggtgcggaac ctggtcaatg
     1741 aataccagaa gcgataccag cgccgcgtct cttttgtgga ccggaacgga cagatcatgg
     1801 tctccggcac gaacttccgg tacgagggaa aaaacatccg cgaagtcccc ggactcgacc
     1861 ggatcgcacg gacggcgctg gcctcggcgg aaaccgctct gcagtacgag gatcggggag
     1921 aaacccatct tctgaatgtc cgctttctcc cggaactggg ctggtacctc tttgtggaac
     1981 ggacggacat cgaagccatc agcgccatcc gttcagcgct cttcctcaat attgttctct
     2041 gtatcgcggt gaccgcgatt gtggccctcc tcgtctccct gaccgtcggc cgctaccaga
     2101 agcgcctcga aaccatggcc agcaccgact ttttgaccgg cttcgtcaac cggcatgcac
     2161 tggccctcct cttcacccag ctgatgaagg acctcaagcg tgtcccccag ccgatctccc
     2221 tgatgatggt cgatatcgac catttcaagc gtgtcaatga cctcttcggc cacctggtcg
     2281 gggactgcgt catccagaaa acagcagccc atatccgctc ggccctgcgg gactccgaca
     2341 tcacgagccg ctggggtggg gaggagtttc tggtcgtgct gcagcgttgc accctgga
//
LOCUS       DAOSHE010000342         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43357_length_2394_cov_3.449765, whole genome shotgun sequence.
ACCESSION   DAOSHE010000342 DAOSHE010000000
VERSION     DAOSHE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43357_length_2394_cov_3.449765"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLD82_08590"
     CDS             complement(<1..1100)
                     /locus_tag="PLD82_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG65477.1"
                     /translation="MLLKNLGGDTILLYGLVQDRGNSSLRLDFAESLKGLTASQYILP
                     MGAGLSGLIAETGQPFHVASLDAEMVEHEIAPIALGKTCYGYPLRTRDHLHSVLIFLF
                     SCDDKILGMRPLLDAIASLLASYMGSVQLRRELFRTRKKFSLLNNYKKLFSGTLDLRP
                     VLSVFMNITASALSAEVGIFVLFKDGTNQTDLEINWGISLQDLSKMEDAQGRNLLTIV
                     LASREIVHIAKYDGGGSGAGKIDSLMAGSLFVQSRPAGILLFANKSLAADGTGRSYFN
                     EEDAELFEVMRDQVANSVENYTLYRKIIEIKNFNENVLESIQSGVVSIDLSGDILTMN
                     RRAEELCGNPLEYPGKHISEMFPVPIFSRESLS"
     gene            complement(1216..1575)
                     /gene="rsfS"
                     /locus_tag="PLD82_08595"
     CDS             complement(1216..1575)
                     /gene="rsfS"
                     /locus_tag="PLD82_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG65478.1"
                     /translation="MSEIDTQEDVFRKAREVAEFLVEKKANNVVCIDISSLTSMADCF
                     IMADVEVQLQIDSLRNQIVDLLSAQGIHARNAVNAVQQGWALLDYNGFIVHIFLTELR
                     EYYGLEKIWGEGKKLFP"
     gene            complement(1562..2299)
                     /gene="recO"
                     /locus_tag="PLD82_08600"
     CDS             complement(1562..2299)
                     /gene="recO"
                     /locus_tag="PLD82_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG65479.1"
                     /translation="MPIKKNERLQALVVKSSPHGESNKIVSLFLSDGRYINGMAHGAR
                     KPASRFGAALEPATLSQVELRYGKRPDDLVTVADGRILNAHTVLKTNLFTMGVLFDFL
                     GLLQYVCQVAGGDEILFRRSVTLLRHCVKNPGEIAGMMPVARLSALLHFGRMPDWSSC
                     TVCHSVECRRVSLHGVYCESCAAKAGEETRPVSAGVLILADIVLRTGIPVGQLPWSEL
                     DGILEWMLEAAHPASEMKRGKTHYVRD"
BASE COUNT          605 a          669 c          578 g          542 t
ORIGIN      
        1 gaaagactct cccgggaaaa aatcggtacc gggaacatct cgctgatgtg tttcccggga
       61 tactccagag gatttccgca gagttcctca gcccgtctgt tcatggtcaa gatgtcacca
      121 gaaaggtcga tcgaaacaac tccgctttgg atactctcca gaacattctc gttgaaattt
      181 tttatctcga tgattttgcg atagagcgta taattctcca ccgaattagc gacctggtcc
      241 cgcatgactt cgaagagttc ggcatcctct tcgttgaaat agctccgtcc ggtaccatca
      301 gcggcgagac tcttgttcgc aaagaggagt atcccggccg gtctggactg gacaaaaagc
      361 gaaccggcca tcagggaatc gatctttccg gcacctgatc cccctccgtc atacttggcg
      421 atgtggacaa tctcccggct tgcaagaaca atcgtaagca ggtttcgtcc ctgggcatct
      481 tccatcttcg agagatcctg gagggatatg ccccagttga tctccagatc ggtctggttt
      541 gtcccgtcct tgaaaaggac aaaaatgccg acctctgccg agagcgcact tgccgtgatg
      601 ttcatgaaca cggacaggac cggtctcaga tcaagagttc cggaaaagag ttttttataa
      661 ttgttgagca gggaaaattt cttccgtgtc cggaacaact cccgcctgag ttggacactt
      721 cccatataac tggcgagaag actcgctatc gcatcaagaa gaggacgcat cccgagaatc
      781 ttgtcatcgc aggagaagag aaagatcaac acactgtgca gatggtccct ggttcggaga
      841 ggatacccgt aacacgtctt cccaagggca atcggagcga tttcatgctc aaccatctct
      901 gcgtcaagtg atgcgacgtg aaagggctgc ccggtctctg cgatcaagcc ggagagtcct
      961 gcgcccatcg gaagaatgta ctggctggct gtcaagcctt tgaggctctc agcaaaatcg
     1021 agccgaagac tcgagtttcc ccgatcctga acaaggccat agaggagaat ggtatctccc
     1081 cccagatttt tgagaagcat ttcaaaaaca tccgggagta cagagcggtg atcccgttca
     1141 gaaagcagga attcggatac gagtgaaacc agttcgtaac cagagacctc tcccgagcgc
     1201 atgcgcatcc cccgttcagg gaaagagttt cttcccctca ccccagatct tttcgagtcc
     1261 gtaatactca cgaagctcgg tcaggaaaat atggacgata aaaccgttat aatccagcag
     1321 cgcccagcct tgctgcacgg cattgacggc atttcgcgca tgaatccctt gtgcagacag
     1381 caggtccacg atctggtttc gcaaactgtc aatctgcaac tgcacctcga catcggccat
     1441 aatgaagcag tcggccatgg atgtcaatga cgaaatatcg atacagacaa cattattggc
     1501 ctttttctcg acaagaaact ctgcaacctc gcgggctttc ctgaaaacgt cttcctgtgt
     1561 atcaatctcg gacatagtgt gtttttcctc gtttcatttc actggcagga tgtgccgctt
     1621 cgagcatcca ttccaggatt ccatccagtt cagaccaagg caattgcccc accggaattc
     1681 cggtccgtag cacgatatcc gccagaatca aaactcctgc actgactggc cgggtctcct
     1741 cacctgcctt ggccgcacag gactcacaat acacgccgtg aagagatacc cgccggcact
     1801 cgacagaatg gcatacggta cagcttgacc aatcaggcat tctcccgaag tgcaacagtg
     1861 ccgagagcct cgcgaccggc atcatgcccg caatctcccc gggattcttg acacaatgac
     1921 gaagaagtgt cacagaacgc cgaaaaagga tctcatcacc gccggcaact tggcaaacat
     1981 actgcaggag cccgagaaaa tcaaacagga cacccatcgt gaacagattt gtcttgagaa
     2041 cagtgtgtgc attcaggatt cttccgtctg caaccgtcac aagatcatcc ggccgtttcc
     2101 cgtagcggag ttcgacctgg ctcagagtcg ccggttccaa agcagccccg aagcgggatg
     2161 ccggttttcg ggcaccatgc gccattccgt tgatgtacct gccgtctgaa agaaaaagac
     2221 tgacgatttt attggactca ccatggggac tgctcttgac gaccaaagcc tggagccgct
     2281 catttttttt gataggcatc tcccttgtca tcctgcttct ccataacctt tccggaaagg
     2341 acaagctgaa tccggatgat cctgttgtca cgaagtgaat gaacccgaag ccgg
//
LOCUS       DAOSHE010000343         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43883_length_2375_cov_2.809052, whole genome shotgun sequence.
ACCESSION   DAOSHE010000343 DAOSHE010000000
VERSION     DAOSHE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43883_length_2375_cov_2.809052"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="PLD82_08605"
     CDS             complement(<1..1987)
                     /locus_tag="PLD82_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65480.1"
                     /translation="MAFGCRIGWCSGLLVLLLFSGGRAVYAAGPALDSRMVLVGDGQA
                     ALKYALSGSAAGSVEGAYLAGNSDSPAGPLFPWLIVEDQLVTPGGRWDDGSPAEGAPH
                     RFGLKSGEEECVLELPCGLKMRTRLAAAGKGVWLWELVVTNHSQEGVTAHAGLAIGST
                     GTNAGAMWRASDGMALIKHRGSFLAIAAVSSSGVLWAPPDKGPLSEAGPKAMQNLSAL
                     YGKNVLNPGMAVRVAFVLVAGTNESEVMGRCQQWMRLDASDKAFPRARAYWENWLAEG
                     RTPFFTDEALQSSMLRSLRTLGIAMHYPGIVPGNPGVLLDCAEAMRSFGRDKEVAGWL
                     DAVKTAIAGDRRATPRGFAEQADGRDILMRYASLVSRQTIASGRLAGDLDLVEQGLAA
                     VSRVVVASNARLRPVDAVQLEAALHDGALLMRLARRKTVSVRMLTNAGLSRVLADRLW
                     SSEARAWTNSAGIPDPRAVADAGLQAGLDFRYDLQYRRLRTFEDVLSLRDRALLVVAG
                     ARAFDLAWYGKALPRWVGASSSLPPLERMALWLRIVAEGSLSGSLAEIDSCSGFRLET
                     LYRIVLEMRHASARVASSFVFRDLRSRLRQHLERAAGRDQAFMCSVGEGLGELRNEMQ
                     KYGLPDRLEQRQVFSRLNRVDRMIAAMIAAEKGLVLDT"
BASE COUNT          561 a          804 c          590 g          420 t
ORIGIN      
        1 cggtatccag aaccaatcct ttttccgctg caatcatcgc ggcaatcatc ctgtctacac
       61 gattgagccg ggaaaacacc tgacgctgct caagccgatc cggcagaccg tacttttgca
      121 tctcgttccg caattcaccc aatccctccc cgacactgca cataaaagct tggtcacgac
      181 ctgccgcgcg ttcaaggtgc tgacgaagcc ggctacgcag atcacgaaag acaaacgagg
      241 atgccacacg cgccgaggca tgccgcattt cgagcacaat ccgatacagc gtttcgagcc
      301 tgaaaccgga gcacgaatcg atttcagcca gactccccga aaggcttccc tcggccacaa
      361 tcctcaacca gagagccatc ctctcgaggg gcgggaggct tgaacttgca ccgacccagc
      421 gcggaagtgc cttcccgtac caggcgagat caaaggctct cgctccggcc acgacaagaa
      481 gcgcgcgatc ccgaagagag agaacatcct caaacgtccg caagcgccgg tattgcaggt
      541 cataccgaaa atcaagcccc gcctggagcc cggcgtccgc caccgccctc ggatccggaa
      601 ttccggcgga attcgtccag gctctggctt cacttgacca gagccggtcg gccaaaaccc
      661 gggacaaccc ggcattggtg agcattcgca ccgatacggt tttccgccgg gccaatcgca
      721 tgagcaaggc tccatcatgg agcgcagcct ccagttgcac agcatcgaca ggccgtaacc
      781 gtgcattgga tgccaccaca acccgactga ccgccgccag gccttgttcg acaagatcca
      841 gatccccggc caagcgacca ctggcaatcg tctgtcgcga aaccagcgaa gcgtatcgca
      901 tcaagatgtc acgcccgtcg gcttgttcgg caaacccgcg cggcgttgct cgccgatcgc
      961 ctgcgatcgc cgttttgacg gcatcgagcc agcctgcaac ctccttgtcc cggccaaagc
     1021 ttcgcatggc ttcggcacag tccaaaagca ctccgggatt ccccgggaca attccgggat
     1081 agtgcatggc aatccccaac gttctcaggc tgcgcaacat cgaactctgg agtgcttcat
     1141 cggtaaagaa gggcgtacga ccttcagcca accagttctc ccaatacgcc cgtgccctgg
     1201 ggaatgcctt gtccgacgca tccagccgca tccactgctg acagcgcccc atcacctcgc
     1261 tctcattggt tcccgcaacc agcacgaagg caacccggac tgccatcccg ggattgagca
     1321 cgttctttcc atacaacgcc gacaagttct gcatcgcttt cggtccggct tccgaaagcg
     1381 gccccttgtc gggtggcgcc caaagcaccc cggacgaaga aactgcggca atcgcaagga
     1441 aagacccacg gtgcttaatg agcgccatgc catcagaagc tcgccacatg gcaccggcat
     1501 ttgtcccggt acttccgatg gcaagcccgg catgcgcagt cacaccctct tggctatgat
     1561 tggtcacaac cagctcccag agccagacgc ccttgccggc tgcagcaagc cgtgttcgca
     1621 tcttcaaacc gcacgggagc tcgagaacac attcctcctc gccacttttc aatccaaatc
     1681 tgtgcggtgc gccttcggcg ggcgacccat catcccagcg accacccggt gtgacgagct
     1741 gatcctcgac gatcaaccag gggaagagag gtccggccgg ggaatcactg tttccagcca
     1801 aatacgcgcc ttcaacagac cctgccgccg aacccgacaa agcatatttg agagctgcct
     1861 gcccgtctcc aaccagtacc atccgggaat ccagtgccgg accggccgca tacaccgctc
     1921 tgccacccga aaagagaaga agaaccagta atcccgaaca ccatccgatt cgacaaccaa
     1981 acgccatcgt gcaaactccc cggcaaaaat gccaatctgt cctttgatac tacaccctgg
     2041 ggtacggttt gcgccaccca tccgtgtcca ctctcttctg tttatacgaa caactccatg
     2101 gatccggcgc attgccggcg cgcaagatcg ccccggaccg gcttgtgtgt tcctcattca
     2161 aaaattgacc gcaagtcaga atatgacgta gctttcaaga aatcatcatg gagtgaaaac
     2221 tgtgggcata accgaaagaa agcagcggga caaggaacgc aggatcaacg agatcctgga
     2281 tgccgccatg cgcgtctttc tggatcgcga tatctcgggc acaacagtgg acgccatcgc
     2341 caacgctgct gaaatcagca aggggacaat ctacc
//
LOCUS       DAOSHE010000344         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43885_length_2375_cov_2.652586, whole genome shotgun sequence.
ACCESSION   DAOSHE010000344 DAOSHE010000000
VERSION     DAOSHE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43885_length_2375_cov_2.652586"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLD82_08610"
     CDS             <1..316
                     /locus_tag="PLD82_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOG65481.1"
                     /translation="VLPEAKLAREAEIAYATLSLVTDYDCWRETGEAVHTEMVVETMG
                     VNNETARRIVRILASHQERLPRECRCSSAAQNAVMTDRRLFPKKTMKKLALLYGKYWT
                     RP"
     gene            313..774
                     /locus_tag="PLD82_08615"
     CDS             313..774
                     /locus_tag="PLD82_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65482.1"
                     /translation="MKATALLTLIFLSAASLRLPALTNTTATNGGYVPLTGQEVFPEQ
                     GSLLMQELGKTGHSESQTRRFEIVFFVSLPVTLMLAYSLMEGLARNTYDRNNPDRTIQ
                     QPHYIYMFSTSVLTSLYIAIEDARRHTVSERPKPTDVSEIRLEFPVVGVRF"
     gene            complement(771..1091)
                     /locus_tag="PLD82_08620"
     CDS             complement(771..1091)
                     /locus_tag="PLD82_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65483.1"
                     /translation="MDIWAKCPKSGEAEAAEREAEAFLRRYGLERFYTLAAVEEMIYT
                     CPRGGEDRLFDPLVARLQGDLAVLYKFDQVFRQRLFSHVPQQSLNGLSPVRFALNLRI
                     MRDG"
     gene            1162..1791
                     /gene="lspA"
                     /locus_tag="PLD82_08625"
     CDS             1162..1791
                     /gene="lspA"
                     /locus_tag="PLD82_08625"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG65484.1"
                     /translation="MKRALPFIILALVVLLDQGSKVLVRDYFQPVDSSELYTMTESVL
                     SASGLGQVDLTIRLPRPPAINLIGEVFWINFHENKGVAFGFMSGLPQSVTVPLFAVIT
                     LLALGFIIHFYRSLPDDKRLPRIALMSITGGAIGNLIDRVAFGKVTDFFDLAQRAPEG
                     YKNLWPIFNVADAFIVVGVAVLFIVLLFEKKHTSKEPDLPSETGEGVSP"
     gene            1788..>2375
                     /locus_tag="PLD82_08630"
     CDS             1788..>2375
                     /locus_tag="PLD82_08630"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG65485.1"
                     /translation="MMPILVTFTFESLFPLVLGLALVFHVLALTLQARARNRKDGSEQ
                     SALRVNSLRSLGNTMLILSVTVLVAFKALHVEKLPLHSYGLMLAIAFLGGIGLAQKRA
                     PLEGIATRDIAHLSIGLIVAALVGSRLFYLVFEVPPQSIMDVLAVWNGGLVIYGGIIC
                     ATGTVWYMMYRKRMPVGRTFDVFAAPLALGIFFGRI"
BASE COUNT          468 a          739 c          626 g          542 t
ORIGIN      
        1 cgttttgccc gaagcgaaac tggcccgtga ggcggagatc gcctacgcca ccctgtctct
       61 ggtcacggac tacgattgct ggcgcgaaac cggggaagcc gtccacaccg agatggttgt
      121 cgagaccatg ggtgtcaata atgaaacagc cagacggatt gtcaggatcc tggcatcaca
      181 ccaggaacgc ctgccccgcg aatgcagatg ttcatccgcc gcccaaaacg cggtcatgac
      241 ggaccggaga ctgttcccca aaaaaaccat gaaaaagctg gcactcctgt atggaaagta
      301 ctggacccgt ccatgaaagc cacagcactc ctcaccctca tcttcctctc cgccgccagt
      361 ctccgactcc cggcattgac gaacacgacg gccaccaatg gcggatatgt gcccttgacc
      421 ggccaggaag tatttcccga acaggggagc ctgctcatgc aggaactcgg gaaaaccggg
      481 cattcggagt cacagacccg gcgctttgaa atcgtgtttt tcgtttcact cccggtcacc
      541 ttgatgctcg cctactccct gatggaagga ctcgcacgga acacctacga ccggaacaat
      601 ccggatcgga caatccagca accgcattat atctacatgt tctcgacttc ggtcctgaca
      661 tcgctctata tcgccatcga agacgcccgt cgccacacgg tcagcgagcg tccaaagccg
      721 acggatgtca gcgaaatccg tctcgagttt cccgttgtcg gtgtccggtt ttaaccgtca
      781 cgcatgatcc gtagattgag ggcaaaacgg accggagaga gtccgttgag ggattgttgt
      841 ggcacatgcg aaaagagccg ttgcctgaaa acctggtcga atttgtagag aaccgcgaga
      901 tcgccctgga gtctcgccac cagggggtca aagagcctgt cctcgccccc gcgggggcag
      961 gtatagatca tctcttcgac ggctgccagg gtgtagaaac gctcgagacc atagcgccgc
     1021 aaaaacgctt cggcttccct ctccgctgct tcggcttcgc cgctcttggg gcacttggcc
     1081 cagatatcca tcggtgtccc cctcttgacc gtattctctg actttgagta tacttgtgat
     1141 aaagcccgag gggaattcct tttgaaacgc gctctaccgt tcatcatcct ggcactcgtt
     1201 gttcttcttg atcagggcag caaggtcctt gtccgtgact acttccagcc ggtcgactct
     1261 tccgagctgt acacgatgac tgaatccgtc ctgtccgcat cgggactggg acaggtggat
     1321 ttgacgatac gcctgcctcg ccctccggcc atcaacctga tcggcgaggt cttctggatc
     1381 aattttcatg aaaacaaggg cgtggccttc ggcttcatga gtggtttgcc gcagtccgtc
     1441 acggtccccc tcttcgccgt catcacactc cttgccctgg gcttcatcat ccatttctac
     1501 cggagtctcc ccgacgacaa acgccttccc cggattgctc tcatgagcat cacgggaggt
     1561 gcgatcggga atctgattga ccgggtcgcg ttcggcaagg tgaccgactt ttttgatctg
     1621 gcgcaacgcg ctcccgaggg atacaaaaac ctctggccca ttttcaacgt ggccgatgcc
     1681 ttcatcgtgg tgggggtggc cgtcctcttt atcgttctcc tgttcgaaaa aaagcacacc
     1741 tcgaaggaac ctgatctgcc atccgaaacc ggtgagggtg tctccccatg atgccgattc
     1801 tcgtcacctt taccttcgag tctctctttc cccttgtcct tggattggct cttgtcttcc
     1861 acgtcctggc attgacgctc caggcacggg cccggaaccg caaggacggg agtgaacaga
     1921 gcgcactgcg tgtcaacagc ctgcgcagtc tcggcaacac gatgctgatt ttgtccgtga
     1981 ccgtcctggt ggccttcaag gccctgcatg tcgaaaagct cccgctccac tcctatggtc
     2041 tgatgctggc cattgccttt ctcggtggca tcgggttggc acagaaacgc gcccccctcg
     2101 aaggaattgc aacccgggac attgcccacc tctccatcgg actgatcgtc gcggccttgg
     2161 tcggttcgcg cctcttttat ctggttttcg aagttccccc gcaaagcatc atggatgtcc
     2221 tcgctgtctg gaatggcgga ctcgtcatct acggtggcat catctgtgcg accgggaccg
     2281 tctggtacat gatgtaccgc aagcggatgc ccgtcggccg gacctttgac gtcttcgcgg
     2341 caccgctggc cctcggaatc ttttttggcc ggatc
//
LOCUS       DAOSHE010000345         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_43904_length_2374_cov_3.189737, whole genome shotgun sequence.
ACCESSION   DAOSHE010000345 DAOSHE010000000
VERSION     DAOSHE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43904_length_2374_cov_3.189737"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PLD82_08635"
     CDS             complement(<1..1277)
                     /locus_tag="PLD82_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65486.1"
                     /translation="MAGQRKTQGNPGPEEKKSRVGQNARVDGHSEKTSTVNKTVKQDG
                     RQSRARASKKVQVKPATNTSAEDVRPLDAREIKKIFSRSTPKQRKGDEGVTEPLPVEE
                     TNPTLPARPWKTIFLVFGPSVLLLLAFALIPGMRETIFGNKTSPVAAERPTNLLPTRY
                     DDRHHATHFFASNQIVAYYGHPQARWMGIVGRYPKETLGPMLRRTAASYDRLNGSRGT
                     VPAFYLIWGSAQPRGEIQFMDKSLLESYIRYTATNGFLLILDHQIGKYGPVNAVEQLL
                     PFLRYPHIHLAIDPEWRTLRPMKEIGSVTGEELNQAQALMKQYMLTNRIPGKRMLIVH
                     QFEDKMYIRRNLVRTTYDPVLFVHSVSGWGSPSMKLGTWNRHTQATNLPNKAFKLWYY
                     PGVNRPGLHYDYPLMSHQTVLGLRPLPILIIYQ"
     gene            complement(1361..>2374)
                     /locus_tag="PLD82_08640"
     CDS             complement(1361..>2374)
                     /locus_tag="PLD82_08640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOG65487.1"
                     /translation="VEMFGYTREEFAGFGSETLYRLSPSDDNELTGSIADNRAKVLEG
                     ARLVFTRVVTRKDGQELWCEVRAQRVPFGERRLVRLSYLDKTLEHGAEEKHKKTEIML
                     LERQKLSAVGTLANGIAHEINNPLMGIINYGQLILDRTVSAEAFVKDYAAEIIGEGTR
                     IAGIVQSLLSFSRQQQDHKESVSAMSLFESIRTLTATIFGKDEISIDFSDEDGLTDLV
                     CTPGRIQQVLLNLITNARDALNERYPGYDPEKTIRVSVCRIILENLKTWARFSVEDHG
                     FGVPEKDRKKLFEPFYTTKPVGSGTGLGLPICYGIIAEHNGRIWYEDGKDGGARFCFD
                     IPL"
BASE COUNT          495 a          665 c          648 g          566 t
ORIGIN      
        1 cattgataga tgatcaaaat tggtaagggt cgcaggccaa gaaccgtctg gtgcgacatg
       61 aggggatagt catagtgcag accgggcctg ttcacccccg gataatacca gagtttgaag
      121 gccttgttgg gaagattggt tgcctgggtg tgcctgttcc atgttccgag tttcatcgag
      181 ggcgagcccc aacccgacac ggaatggaca aacaaaaccg ggtcataggt cgtccggacc
      241 aggttccgcc tgatgtacat cttgtcctcg aattggtgca caatcagcat cctcttcccc
      301 ggtatgcgat tcgtgagcat gtactgcttc atgagcgcct gcgcctgatt gagctcttct
      361 ccggtcacgg agccgatttc cttcatcggc ctgagcgtcc gccattcagg atcgatggcg
      421 agatggatgt ggggatagcg caaaaacgga aggagttgct cgaccgcatt gaccggacca
      481 tacttgccga tctggtggtc cagaatcagg aggaacccgt tggtcgccgt ataccggata
      541 tagctctcaa ggaggctctt gtccatgaac tggatttcac cgcggggctg ggccgaaccc
      601 cagatcagat aaaatgcggg aacggttccc ctgcttccat tgagccggtc atagctggcg
      661 gccgtccgtc gcagcatcgg tccgagggtc tccttgggat agcgtccgac aatccccatc
      721 cagcgggcct ggggatggcc gtaataggcc acgatctggt tgctggcaaa aaaatgtgtg
      781 gcgtgatggc ggtcatcgta ccgggtcggc aggagattgg tcggtcgttc agccgccacc
      841 ggagacgtct tgttcccgaa gatcgtctcc cgcataccgg ggatcagggc aaacgccaaa
      901 aggagcagga ccgatggccc gaacacgagg aaaatggtct tccatggtcg tgccggaagc
      961 gtggggttgg tctcctcgac cggcaagggt tcggtgacac cctcatcgcc cttccgctgt
     1021 tttggggtcg acctggaaaa gatttttttg atttcccggg catccagagg gcgaacatct
     1081 tccgccgatg tgttcgtggc cggtttgacc tggacttttt tggaagcccg cgcccgggac
     1141 tgtcgtccgt cctgtttgac ggtcttgttc accgtcgaag tcttttcgga gtgcccgtca
     1201 acccgtgcat tctgtccgac ccgggatttc ttttcctccg gtcccggatt gccttgtgtt
     1261 ttgcgctgtc ctgccatcgt tctcccctgc tcttgcacag gaaagatact gctcccgggg
     1321 aacaacgaaa acgaggggat tcaccgagtt cagggcaggt tcacaacggt atatcgaagc
     1381 agaacctggc ccctccgtcc ttgccatcct cataccagat ccgaccgttg tgttcggcga
     1441 taataccgta acagatcggc aatccgagtc cggtaccgct gccaaccggc ttggttgtgt
     1501 aaaatggctc aaacaatttc ttgcgatcct tttcagggac accaaatccg tgatcctcaa
     1561 ccgaaaatct ggcccaggtt ttgaggtttt cgagaatgat ccggcacaca gaaacccgga
     1621 ttgtcttttc tgggtcatat ccgggatagc gctcattcaa ggcatccctg gcattcgtga
     1681 tcagattcaa caaaacctgc tggatccttc ccggggtaca gacgagatcc gtcagaccgt
     1741 cttcgtcaga aaaatcgatc gaaatctcgt ccttgccgaa gattgtcgcc gttagtgtcc
     1801 ggatcgattc aaaaagagac atggccgaaa ccgattcctt gtgatcctgc tgctgtcttg
     1861 aaaaagagag aaggctctgg acaatccccg cgattctggt tccttcaccg atgatttctg
     1921 cggcatagtc tttgacgaag gcctctgcgg acacggtccg gtcgagaatc aactgcccat
     1981 aattgatgat ccccatgaga ggattattga tctcgtgcgc gatcccgttg gccagtgttc
     2041 cgacagcgga caatttttgg cgctccaaaa gcatgatttc cgtttttttg tgtttctctt
     2101 cggctccgtg ttcgagcgtc ttgtccagat agctcaagcg gacgagtctc cgttcgccaa
     2161 acggcacacg ctgtgcgcgc acctcacacc ataattcctg tccgtccttg cgcgtcacaa
     2221 cccgggtaaa gacaagacgg gcaccctcaa gcaccttggc ccgattgtcc gctatcgaac
     2281 cggtcagttc gttgtcatcg gatggactca acctgtagag cgtctccgat ccgaatccgg
     2341 caaactcctc acgggtatag ccgaacatct cgac
//
LOCUS       DAOSHE010000346         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44027_length_2369_cov_3.365601, whole genome shotgun sequence.
ACCESSION   DAOSHE010000346 DAOSHE010000000
VERSION     DAOSHE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44027_length_2369_cov_3.365601"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PLD82_08645"
     CDS             <1..139
                     /locus_tag="PLD82_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG65488.1"
                     /translation="GRGSCISECPVDAISEGTPYVIDPSLCTDCGSCAAVCPVSAISP
                     G"
     gene            241..468
                     /locus_tag="PLD82_08650"
     CDS             241..468
                     /locus_tag="PLD82_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG65489.1"
                     /translation="MQLCFAPRGTTLILKDVLGGHEVRHRLAEMGMVSGTELELLQAS
                     GSGPVMVRIGGSKIGIGRGMAQRIEVSVKEE"
     gene            535..1716
                     /locus_tag="PLD82_08655"
     CDS             535..1716
                     /locus_tag="PLD82_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65490.1"
                     /translation="MSVATTPRILFVDDEDSLRFLFCKVFEKKPWVIDTASDFGEALK
                     ALSEKNHDVVITDITMPGKSGLDLLRDIHAINRDILVILITGNPSVESASEAVRHGAY
                     DYLIKPFNMTSLEYVVENALQQKKQQDEKKRLEIIEHNYIDNLERTLHAQTLEIRKAY
                     KFLSQAHAKSLQILARAADYRDDDTGTHIAKIGEYSFILAKHMGLPAKDAEILRYAAP
                     MHDVGKIGIPDSILQKPGSLTAEEFEAIKRHTIIGAQIFFDSAHPYHIASGIIALTHH
                     ERYNGTGYPRGISGDDIHLFGRIVAVADVFDALITPRVYKEQWSVEKAVDLIRSESGK
                     HFDPKVCACFLDTIEEILETGKKIESEEEQRKSSFNLDSLLAKVPASDHIDEDDDSWN
                     Q"
     gene            1704..2354
                     /locus_tag="PLD82_08660"
     CDS             1704..2354
                     /locus_tag="PLD82_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG65491.1"
                     /translation="MEPVTFDLVIFDLDGTLTDTSLDLTNAINLARRELGLPDLAVAD
                     VVAAVGNGAQRMVERIFSDHPELILEALEIYRRTYAACRTETVCLYPGVLETLDALRQ
                     KNIPMGVISNKSEDSAREVLRHLSVDRYFFAVLGDDGKRPLKPDPAPVHEILMTSGCD
                     PVACLMVGDSRVDIECARNAGIRMAFLADRMGQAHQTLVPDFTMRDTREILTLFAG"
BASE COUNT          531 a          663 c          655 g          520 t
ORIGIN      
        1 cggccgcgga agctgcatca gcgaatgccc cgtagacgcc atcagcgaag gaacgcccta
       61 cgtcatcgac cccagcctgt gcactgactg tggcagttgt gccgctgttt gccccgtcag
      121 cgccatctca cccggctgac gtacggcggg gccgtttcat tcctgaaacg gccccatctc
      181 ttgcattcag gactctcatc aatgcacact gtatcaccaa cgattgtcag gacacgttcc
      241 atgcagcttt gttttgctcc acgcggtaca accctcattc tcaaggatgt tctgggcggg
      301 cacgaggttc gccacaggct tgccgagatg gggatggtgt ctggcaccga gcttgagctt
      361 ctccaggctt ccggaagcgg ccccgtcatg gtccgtatcg gcggaagcaa aatcgggatc
      421 ggccgcggta tggcccaacg gatagaagtc agcgtcaaag aggaatagtc tccctcttgc
      481 aacctgtgtc tggcgtggta tactcactcg aagagtgaag gagacctgcc caccatgtcc
      541 gttgctacga ctccccgcat cctctttgtc gacgatgagg acagcctgcg gtttctcttt
      601 tgcaaggtct ttgaaaagaa accttgggtc atcgataccg ccagcgattt tggtgaggcc
      661 ctcaaggcac tttccgaaaa gaatcacgat gttgtcatta ccgatatcac catgcccgga
      721 aagagtggtc tggatcttct cagggacatc catgccatca accgggacat ccttgtcatc
      781 ctgatcacgg ggaacccctc ggtcgagtcg gcttccgaag cggttcgaca cggggcctat
      841 gactatctga tcaaaccctt caacatgacg tcgctcgaat atgtcgtcga gaatgccctg
      901 caacaaaaga agcagcagga cgagaagaag cggctcgaga tcatcgagca caattacatc
      961 gacaaccttg agcggacact gcatgcccag actctcgaga tccgcaaggc ctacaaattt
     1021 ctctcccaag cccatgctaa atccctgcag atcctggcga gggcggcgga ttatcgggat
     1081 gatgacaccg ggacccatat cgccaagatc ggcgagtaca gcttcattct ggccaaacac
     1141 atgggactcc ccgccaagga tgcagagatt ctgcgctatg ccgcaccgat gcatgatgtc
     1201 ggaaagatcg gaatcccgga cagtattctc caaaagcccg gaagtttgac agccgaggaa
     1261 ttcgaggcca tcaaacgcca cacgatcatc ggggcccaga tcttttttga ttccgctcac
     1321 ccgtatcaca tcgcctcggg aatcatcgcg ctgacccatc atgaacgcta caacggaacc
     1381 gggtatccaa gggggatctc cggcgacgat atccacctct ttggccggat tgttgccgtc
     1441 gccgatgtct ttgatgcctt gatcacaccc cgtgtctaca aggagcagtg gtcggtggaa
     1501 aaggctgtcg atctgatccg ctccgaatcg ggaaagcatt ttgatcccaa ggtctgcgcc
     1561 tgttttcttg atacgatcga ggagatactc gaaaccggga agaagatcga atcagaggaa
     1621 gaacaacgga aatccagttt caatcttgac tcactgttgg ccaaggttcc tgccagcgac
     1681 cacattgacg aagatgatga ttcatggaac cagtgacttt tgatctcgtg atctttgatc
     1741 tcgacggaac gctgactgat acgtctctgg acctgaccaa cgccattaat ctcgcccggc
     1801 gggaactcgg cttgccggac ctcgcggttg ccgatgttgt ggctgccgtc gggaatgggg
     1861 cacaacgcat ggtcgagaga atattttcgg atcatccgga gctcatcctg gaggccttgg
     1921 agatctaccg gcgtacctat gccgcctgtc ggacggagac ggtgtgtctg tatcccggag
     1981 tcctcgagac tctggatgct ctcaggcaaa aaaatattcc gatgggtgtc ataagcaaca
     2041 agtcggaaga ctcggcacgg gaggtcttgc gtcatttgtc ggtcgacagg tatttttttg
     2101 cggtcttggg tgatgatgga aagcggccac tcaagccgga tccagcgccg gtccatgaga
     2161 ttctcatgac gagtggatgt gatccggtgg cttgcctgat ggtgggagac agtcgggttg
     2221 atatcgagtg tgcgcggaat gccgggatcc ggatggcctt tctggccgac cggatggggc
     2281 aggcccacca aaccctggtg ccggacttca ccatgcggga cacacgggag attctgacac
     2341 tctttgccgg ttgatgcgtg ccacgatca
//
LOCUS       DAOSHE010000347         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44202_length_2363_cov_3.614385, whole genome shotgun sequence.
ACCESSION   DAOSHE010000347 DAOSHE010000000
VERSION     DAOSHE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44202_length_2363_cov_3.614385"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLD82_08665"
     CDS             <1..1155
                     /locus_tag="PLD82_08665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65492.1"
                     /translation="MKLQVVEDEEVARMQMVRTLSRWFDNIVAVPDGDAAWREIRNGE
                     YDIVITDWLMPGIDGLTLCHMIRSLEASKYVYVILVTSRDGKEMLVRGLEAGADDYIV
                     KPYEMDELRVRVQAGKRIISLERELSEKNNLLLSELETSVHTLRDMLPDRARRWGGRV
                     QIDWLYQPSVYIGGDFFNVIELTEQHLAFYLLDVSGHGVSAALSAVSVSSLLNSSGSR
                     EGLLYDEQGAPASPALVASRLNQRFQCKGPRGFYFTIFYAVLDLQTLSMRYIRAGQTC
                     PLVMRQGQCITVDEGTIPVGFFPEHTYTDNEMQMQIGDTIILFSDGVNETSDAEGIMF
                     GVERIEKHFMTLEKEESTDVLERLAGQLRRYSGLPGFEDDVTVLMLDIVR"
     gene            complement(1324..1662)
                     /locus_tag="PLD82_08670"
     CDS             complement(1324..1662)
                     /locus_tag="PLD82_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOG65493.1"
                     /translation="MSLFDQINTAENFNEDVTKEKHLPTITATKLEGNRYRVRIDVGQ
                     GKHPNEIDHWIQWTELRVQGLFIGRAEFSAKIMEPIAEFVVNLEAPASISALSRCNKH
                     GLWETSIKVG"
     gene            complement(1749..2150)
                     /locus_tag="PLD82_08675"
     CDS             complement(1749..2150)
                     /locus_tag="PLD82_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65494.1"
                     /translation="MRKFHFLFLSFLMGVFLLSCGASRLSREEAARLSALPEKKIAER
                     GLTYFIEKNYDLAIDAYSLILARTNADKRYAAWARYETGFCYYYMSDYDKAMEHFRIL
                     LRDFPQREYVNQRTLAEMLINKIEKGLTDGI"
     gene            2287..2359
                     /locus_tag="PLD82_08680"
     tRNA            2287..2359
                     /locus_tag="PLD82_08680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2320..2322,aa:Ala,seq:ggc)
BASE COUNT          524 a          609 c          664 g          566 t
ORIGIN      
        1 atgaagcttc aggtcgtcga ggatgaggaa gttgcccgga tgcagatggt ccggaccctg
       61 tcccgctggt ttgacaatat cgttgcggtc cccgacgggg atgctgcctg gcgcgagata
      121 cggaatggtg aatatgacat cgtcatcacg gactggctga tgccggggat agacggtctg
      181 acgctctgcc acatgatccg cagtcttgag gcatcaaagt atgtctacgt cattctggtc
      241 acatcccgtg acggcaagga gatgctcgtc cgcggactcg aagccggcgc cgacgattat
      301 atcgtcaaac cgtacgagat ggatgaactc agggtccggg tccaggccgg aaaacggatc
      361 atcagcctgg aacgggaact ctccgaaaaa aacaacctgc ttctctccga gcttgagacc
      421 tcggtccata ccctccgcga tatgcttccc gacagggcac ggcgctgggg tggtcgtgtg
      481 cagatcgact ggttgtatca gccatcggtc tatattggtg gcgatttttt caatgtgatc
      541 gaactgaccg agcagcatct cgctttctac cttcttgatg tctcgggcca tggggtttca
      601 gccgcactct ccgcagtcag tgtttccagt cttctcaaca gttctggttc tcgggaaggc
      661 ttgctctacg atgagcaggg cgctccggca tccccggcac tggttgcgtc acgcctcaac
      721 caacgattcc agtgcaaggg gcctcgtggt ttctatttca cgatttttta cgccgtcctc
      781 gatcttcaaa cgctctccat gcgctatatc cgtgccgggc aaacatgtcc cctcgtcatg
      841 cggcaggggc agtgtatcac ggttgacgag ggcacaatcc cggtcggctt ctttccggag
      901 catacgtaca cagacaacga gatgcagatg cagatcgggg ataccatcat tctcttttcc
      961 gatggtgtca acgaaacatc ggatgccgaa ggcatcatgt tcggtgtgga gcggatcgag
     1021 aagcacttca tgacgctcga aaaggaagag agcacggatg tgctcgaacg cctggccggt
     1081 cagttgcggc gctactccgg tcttccgggg ttcgaagacg atgtgaccgt cctcatgttg
     1141 gacattgtgc gctgaccgtc tccggagagt ccgtattttt ttcgatcggt ttgaggaaag
     1201 cgtgccggtc ttgaaaacat ttttggtgaa cagctggctg tcaccggacg caggggtctg
     1261 gcatgcaaaa catcccggcc ggcaccggcg ctggatgttc cagacaggtt tgtccgcaag
     1321 aagtcacccg actttgatcg acgtctccca gagtccatgc ttgttgcagc ggctgagcgc
     1381 actgatgctg gccggtgcct caagattgac gacgaattca gcgattggtt ccatgatttt
     1441 ggctgagaac tccgcgcgac cgataaagag tccctggacg cggagctcgg tccactggat
     1501 ccagtgatcg atttcgttgg ggtgctttcc ctggccgaca tcaatgcgga cccggtagcg
     1561 gtttccctcg agctttgtgg ccgtgatcgt ggggagatgt ttttcttttg tgacatcctc
     1621 gttgaagttt tctgccgtgt tgatttgatc aaagagactc atatccgacc tcctggtggt
     1681 tcattatggt aaaatatagt aaaaaaagca tactgacggg ccgggaccgg taaaaacagg
     1741 ggatccgatc agattccgtc agtcagacct ttttcgattt tattgatcag catctcggcc
     1801 agtgtccgct gattcacgta ctcccgttgc ggaaaatcac gaaggagaat cctgaaatgc
     1861 tccatggctt tgtcgtagtc ggacatgtag tagtagcaaa agccggtttc atagcgcgcc
     1921 caggcggcat aacgcttgtc cgcattggtc ctggcaagga tcagggaata ggcatcgatc
     1981 gcaagatcat aatttttttc tatgaaatag gtcagtccgc gttcggcaat ctttttttcg
     2041 gggagggcgg agagtctggc tgcttcttca cgggagagac gggaagcccc acaggaaagc
     2101 agaaaaactc ccatcaaaaa cgaaagaaac aggaaatgaa actttctcac ggataactcc
     2161 ttctggcgcg gcgtgtccaa agtatactcg tcccggtacg ataaagaaaa ttcaaaaaaa
     2221 actggactca aaggctgctc cgaagtaaat tattctcgtc cgcaacggtc atgaccgggc
     2281 agatgcgggg gtgtagctca gttgggagag cgcttgaatg gcattcaaga ggtcaggggt
     2341 tcgactcccc tcatctccac acc
//
LOCUS       DAOSHE010000348         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44347_length_2357_cov_3.572980, whole genome shotgun sequence.
ACCESSION   DAOSHE010000348 DAOSHE010000000
VERSION     DAOSHE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44347_length_2357_cov_3.572980"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLD82_08685"
     CDS             complement(<1..936)
                     /locus_tag="PLD82_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG65495.1"
                     /translation="MSTIEMTLPDGSVRQVESGTTCLDFAKSIGRKLAEDALAVELDG
                     VLRDLGTPLTAPASLRFITFESDVGHDLFWHSSSHVMAQAVCRLFPGTKYTIGPSIEN
                     GFYYDFDSEHVFTPEDLAAIEAEMAKIATEKIAFVRRELPRDEAVAVFKKLGNEYKTE
                     IISELPEGSVISLYEQGDFIDLCRGPHVPNTGYVKATKLLSLAGAYWRGDQKNRMLQR
                     IYGISFPAKKLLDEWVLRFEEAKKRDHRKIGREMDLFSFQDEGPGFPFWHPNGTIVFN
                     ELVDYIREKNTARGYGEVRTPPILNESLWHTSGHWD"
     gene            complement(1065..1138)
                     /locus_tag="PLD82_08690"
     tRNA            complement(1065..1138)
                     /locus_tag="PLD82_08690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1102..1104),aa:Val,seq:gac)
     gene            complement(1154..>2357)
                     /locus_tag="PLD82_08695"
     CDS             complement(1154..>2357)
                     /locus_tag="PLD82_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOG65496.1"
                     /translation="WQILLARFGRGRLLEGLPKHAVWLHAVSGGEVAATALLYPALRK
                     ALGDQIPIILTCTTASGMSMAGKRFPQAVRCLYAPLDTPGAVRRFFQKLEPTILAVAE
                     IEIWPNLFREAAQRDVPLVMYNARMPDADLGKYARFRWIFKHVLSTVRMIAAQTAEDA
                     ARYCAIGAPADRITVTGNIKNDQEYRNPPERVFRFDQSDPRPVILLASSHPGEEQAVL
                     DAVMTLGARLVVAPRHVQRARKIAALLRKSGYSFVVRSSLEAEGAVITTDVLLLDTFG
                     ELMSAARESLLVIMGGSFTRRIQGHNPMEAAALSRPVILGPCMDNFRDVRDALLAGGG
                     AVSVSGYSAVRDAVQAWVDNPSDAERSGKQARIVLESLRGASARTAEIMATQLVGRKK
                     FDSFHLSS"
BASE COUNT          540 a          696 c          607 g          514 t
ORIGIN      
        1 gtcccaatgg ccgctggtat gccagagcga ctcattgaga atcggaggag tccgtacttc
       61 accgtacccc cgggccgtat tcttttcccg gatatagtcc accagctcat tgaagacaat
      121 cgttccgttg ggatgccaga agggaaaccc cggaccttca tcctggaacg aaaagagatc
      181 catctcccgg ccaatcttcc ggtggtcacg cttttttgcc tcttcaaacc gaagaaccca
      241 ttcgtcgagg agtttttttg cgggaaacga gatgccatag atccgttgca gcatccggtt
      301 tttctggtca ccgcgccaat aggctccagc cagagaaaga agctttgtcg ccttgacata
      361 tcctgtattg ggaacatggg gcccgcggca gagatcgata aaatcgccct gctcgtagag
      421 cgaaatcacg gaaccctcgg gcaattcact gatgatctct gttttgtact cgtttccgag
      481 tttcttgaaa accgcgacag cctcgtctct cggaagttcc cggcgcacaa aggcgatctt
      541 ttctgtcgct atcttcgcca tctccgcttc aatggccgcc agatcttcag gtgtaaacac
      601 atgttcactg tcgaaatcgt aataaaaccc gttttcgata gacggcccga tcgtgtactt
      661 tgttcccgga aaaagtctgc agacagcctg cgccatgaca tgggaggagg aatgccagaa
      721 gaggtcatgc ccaacgtcac tctcgaacgt gataaaccgc agactggccg gtgcagtcaa
      781 gggggttcca aggtcacgga ggaccccgtc caattccact gccaaggcgt cctcagccaa
      841 cttccggccg atcgatttgg caaaatccag acaggttgtg cccgattcga cctgacggac
      901 actcccgtca ggcaaggtca tctcaatggt gctcatatcg ctgcctcact tctgcgacta
      961 cctgattgaa gtgcgaaaaa ccccgctctt ccgggccata tacaacagcc tctcgtggaa
     1021 ggggcggggt cgttcccggt agttttcgcg ctctcgtacg actgtgggcg atactggatt
     1081 tgaaccagtg accccttgca tgtcaagcaa gtactctaac caactgagct aatcgcccgt
     1141 acgtttctat aaattagcta ctcaggtgaa aagaatcaaa tttttttctg ccgacgagtt
     1201 gcgttgccat gatttcggct gttctggccg aggctccacg cagactctca agcacgatgc
     1261 gcgcctgctt gccggaccgc tccgcatccg acgggttatc gacccaggcc tgaaccgcat
     1321 cccggaccgc tgaatacccg gaaaccgaaa cggccccgcc cccggccagg agggcgtctc
     1381 tgacatcccg aaaattgtcc atacacggac caagaatcac cggacgggag agcgctgccg
     1441 cttccatcgg gttgtgcccc tggatcctac gggtaaacga acctcccatg atgacaagga
     1501 gtgactctct ggcggcagac ataagctccc cgaaggtatc caacagcagg acatccgtcg
     1561 tgatgacggc tccttccgcc tccaatgagg accgcacgac aaaggaatat cccgactttc
     1621 gcagcaaggc tgcgatcttc cgtgcacgct gtacatggcg gggtgccacg acaagccgtg
     1681 caccaagtgt catcactgca tccagcactg cttgttcttc gccgggatgg gaagatgcca
     1741 gaaggatgac cggcctcgga tccgattggt caaaccggaa aacacgctca ggaggattcc
     1801 tgtattcctg gtcatttttg atattcccgg tgaccgttat tctgtccgct ggagcaccga
     1861 tggcacaata gcgtgccgca tcctccgcgg tctgtgcagc tatcatcctg acggtcgaca
     1921 acacgtgctt gaagatccag cggaatctgg catatttccc cagatctgca tccggcatgc
     1981 gggcattata catgaccaga gggacatccc gctgtgcagc ctctctgaac agatttggcc
     2041 agatttcgat ttccgcaaca gcgagaatcg tcggttccag cttttggaag aatcttcgga
     2101 ccgctccagg ggtatcaagc ggggcataca aacacctcac tgcttgagga aaccgtttcc
     2161 ctgccatgga catgccgctt gctgttgtgc acgtgagaat gatcggaatc tggtccccca
     2221 gagctttgcg taaagccggg tacaagagcg ccgttgcagc aacctcgcca ccagaaacag
     2281 catgcagcca aaccgcgtgt tttggaaggc cctcgagcaa acgaccgcgg ccaaatctgg
     2341 cgagaaggat ctgccag
//
LOCUS       DAOSHE010000349         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44350_length_2357_cov_3.321025, whole genome shotgun sequence.
ACCESSION   DAOSHE010000349 DAOSHE010000000
VERSION     DAOSHE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44350_length_2357_cov_3.321025"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2357
                     /locus_tag="PLD82_08700"
     CDS             <1..>2357
                     /locus_tag="PLD82_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG65497.1"
                     /translation="QIREEVDLWRNKGYRAPAGISPTSQRLLEWWFDETHRFENGESF
                     QYYFAQQEAIETIIYLYEVKGYRDVADMILKYMDPKAYKEDLFTRRKTIEQTIRNERI
                     LTRVVPETGLVAQQELPQPDHSRFAMKMATGSGKTLVMAMAAVWSYFHKKFEKRSSLS
                     RTFLVIAPNIIVFERLREDFENGKIFKDWDLIPPEWQHDWQMSFILRGESRKTTTEGT
                     LYLTNIHQLYEDKGNNQPGDPVNRLLGPKPKEATGSWEEEILERVRKHHDLLIINDEA
                     HHVHDTDLEWYRVILNLHKNLKEKFGARLSLQLDFTATPKDQNGTFFPWIVCDYPLAQ
                     AIEDRIVKSPLIVHKTDRADPDKYSKASVAYAEWINIAISRWQEHFEAYSKVGKRPVL
                     FIMAENTRDADDIFDFLKGKAEFKGGGQLLLIHTDKTGEVSKKDLEAAREAARSIDLA
                     KNKIRAIVSVMMLREGWDVRNVSVILGLRPFSAKANILPEQAVGRGLRLMRGVGPDYI
                     QIVEIIGTQKFEEFVKNLEVEGVGVGITKDPPPLGVHIYPMKIKADYNIEIPILSPVH
                     TREFKGLDDSVIRGLLANPKGLTQISKEKTTVTLTETITQKKVGQKQVTIDTGIPEIN
                     EILTHLTTRICKEARIDGQFAVVYPIVRKYVREIFFGQEVDLETEQIRRLLSNAANRD
                     HIMSTLAKAIGEKSISKITSTLRSDPLSLMELDGFYWRRDWCELDKTVFNVTPCFNPF
                     EKHFAQFLEQAGDIERFAKLAESYTKFSIEYLNHKGAISTYYPDFV"
BASE COUNT          604 a          649 c          644 g          460 t
ORIGIN      
        1 cagattcgcg aggaagtgga tctgtggcgc aataaaggct atcgggcgcc tgccgggatt
       61 tcgccgacct ctcagcgcct gttggagtgg tggttcgatg aaacccaccg cttcgaaaac
      121 ggcgagtctt ttcaatatta ctttgcccag caggaagcga tcgagacgat catttacctt
      181 tacgaggtca agggctacag agacgttgcg gacatgatcc tcaagtacat ggaccccaag
      241 gcctacaagg aggatctctt cacccggaga aaaaccatcg agcaaaccat ccgaaacgag
      301 cgcatcctca cccgcgtcgt cccggaaacg gggcttgtcg cccaacagga gctgccccag
      361 cccgaccata gccgttttgc catgaagatg gcgacaggct cggggaaaac gctggtcatg
      421 gcaatggccg cggtctggtc ttatttccac aaaaaatttg aaaagagatc cagcctcagc
      481 cggacgtttc tcgtgatcgc ccccaacatc atcgttttcg aacggttgcg ggaggatttt
      541 gaaaacggga agatcttcaa ggactgggac ctgatccccc cggaatggca gcacgactgg
      601 cagatgtcct tcatcctgcg aggggaatcg aggaagacga cgacggaagg gaccctctat
      661 ctgacgaata tccaccagct ctatgaagac aagggaaata atcagcccgg agacccggtc
      721 aatcgcctct tggggcccaa accgaaggag gccaccggat cctgggaaga ggaaatcctg
      781 gaacgagtcc gcaagcacca tgatctgctc atcatcaacg acgaggccca tcatgtccac
      841 gacacggacc tggagtggta cagggtcatt ctgaatcttc acaagaacct gaaggaaaag
      901 ttcggcgccc ggctttcgct gcaactggat ttcaccgcaa cgccgaagga tcagaacggc
      961 accttcttcc cctggatcgt ttgcgactac ccgctcgctc aggctatcga ggaccgcatc
     1021 gtgaagtcgc ccctgatcgt ccacaagacg gacagggccg atcccgacaa atattccaag
     1081 gccagcgtcg cctatgcgga atggatcaac atcgccatct cccgctggca ggagcacttc
     1141 gaagcctaca gcaaagtcgg aaagcgtccg gtgttgttca tcatggcgga aaacacgcgg
     1201 gatgccgacg acattttcga tttcttgaag ggaaaggccg agttcaaagg cggcgggcaa
     1261 cttctcctca ttcacacgga caagaccggc gaggtgagca agaaggatct tgaggcggcg
     1321 cgggaggcgg cgcgttccat cgatttggcg aaaaacaaga tcagagccat cgtcagtgtc
     1381 atgatgctcc gggagggttg ggatgtgcgg aacgtctccg tgattctggg gctccgccct
     1441 ttcagtgcca aggccaacat tctgcccgaa caggccgtcg gccgggggct ccgcctgatg
     1501 aggggggtcg gacccgatta catccagatc gtggagatca tcgggacgca aaaattcgaa
     1561 gagttcgtca agaatctgga ggtggagggc gtcggcgttg gcatcacgaa agacccgccg
     1621 cctcttggtg tccatattta tccgatgaaa atcaaggcgg attacaacat cgaaattccc
     1681 atcctctccc ctgtgcatac gagagaattc aagggattgg atgattcggt catccgggga
     1741 ttgctcgcca acccaaaggg cctgacccag atatctaaag agaaaacaac ggtcacgctc
     1801 actgaaacaa tcacacaaaa gaaggttggc cagaagcaag tgacgattga tacaggcata
     1861 ccggaaatca acgagatcct cacccacctg acaaccagga tctgcaagga ggccaggatc
     1921 gacggacagt ttgccgtggt ctatcccatc gtgcggaagt atgtccggga gatcttcttt
     1981 ggtcaagagg tggacctgga aacggaacag atccgacgat tgttgtcaaa cgcggcaaac
     2041 cgcgaccaca tcatgtctac actggcgaaa gccatcggtg agaagagcat ctccaagatc
     2101 acctcaacat tgagatccga tcctctttcc ctgatggagc tggatggctt ttactggcgg
     2161 cgggattggt gcgaactgga caaaaccgtc ttcaatgtga ccccctgttt caatcccttt
     2221 gaaaaacact tcgcccaatt cctggagcag gccggggaca tcgagcggtt cgcaaaactc
     2281 gccgagtcct acacgaaatt ctccatcgag tacctgaatc ataagggtgc catatcgacc
     2341 tattaccccg attttgt
//
LOCUS       DAOSHE010000350         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44589_length_2349_cov_3.315170, whole genome shotgun sequence.
ACCESSION   DAOSHE010000350 DAOSHE010000000
VERSION     DAOSHE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44589_length_2349_cov_3.315170"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PLD82_08705"
     CDS             <1..1855
                     /locus_tag="PLD82_08705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65498.1"
                     /translation="RFTGGRKVAPQIQRMKSDLSSCFQLAESPAVRTWLKNEFDPTAR
                     AQAFGTFDAFSKNMENNPAVYAVVSSSLRYYLKNEYQSLISKKNPVDDWYFRTISRPE
                     RFMLDIDYDPGLDLTRFWINVPVHETAGDAGSAARYNDTLGVVGTGLDISVFLKTMLR
                     IEDTNRDEEKKLKNAFIAFFNSRGIIMAYQNVNCINRTSIFSLLDIPNERTALSNLMT
                     TVRNSESVSNTPPSLPLEHHGTRFHASLVWIPMIDWYALVFIDTSELISFDRFAPLIA
                     IISAALVTLFLAMLFFTNNRLIKPIVRLSAIMTRMQGGDLEVRAEATGHDEIGELALA
                     CNAMADEIKGYTSRLEEMVRKRTEELVRKNHEMVDSISYARLIQSGMLPKRSRISHRI
                     SDHSIIWRPRDIVSGDFYWFKEVEDILYLAVVDCTGHGVPGAFMTMTANAVLDHVIWY
                     LNDDPAAMLMELDRLMRTTLNQDDASDAQSNDGLDIALVMIQPEKHSFVYSGARIPLF
                     FMHKGKVEMVRGDRQSIGYTNSEQVLSFTAHEINYVPGDRLFISTDGLFDQTGGDRAK
                     PFGTTRFSELILETAGLDLEGQRNRILMELDTHAGGGGQLDDITVFALALP"
     gene            complement(2152..>2349)
                     /locus_tag="PLD82_08710"
     CDS             complement(2152..>2349)
                     /locus_tag="PLD82_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65499.1"
                     /translation="LLMTGLGSTLVDTSTMDLAFRAGALLRFEFVDWLRLDAGVRIEF
                     INTMGGATVIGGPVLRVSLVL"
BASE COUNT          525 a          710 c          631 g          483 t
ORIGIN      
        1 gcgttttacc ggcgggcgca aggtggcgcc gcagatccag cggatgaaaa gcgatctctc
       61 gtcctgtttt cagctcgccg aatcaccggc ggtacggacc tggctgaaaa acgaattcga
      121 cccgacagcc cgggcccagg ccttcgggac ctttgacgcc ttcagcaaaa acatggaaaa
      181 caatccggcc gtctatgccg tcgtctcgtc gtcattgcgc tactacctca agaacgaata
      241 ccagagcctg atctccaaaa aaaatcctgt ggacgactgg tatttcagga ccatctcccg
      301 tccggaacgc ttcatgctcg atatcgacta tgatccgggc ctcgatctga cccgcttctg
      361 gatcaatgtc cccgtccatg aaacggccgg agatgcgggc agcgctgccc gttacaacga
      421 tacgctgggt gtcgtcggaa ccggactgga tatctcggtc ttcctcaaaa caatgctccg
      481 catcgaagac acaaacagag atgaggagaa aaagctcaaa aatgccttta tcgctttttt
      541 caactccaga ggcatcatca tggcctacca gaacgtcaac tgcatcaacc gcacgtcgat
      601 tttctccctt ctggacattc ccaacgaacg gaccgcgctc tccaacctga tgacgacggt
      661 ccggaacagc gaatccgttt ccaatacgcc cccgtccctc ccgctcgagc atcatggtac
      721 acgattccat gcctctcttg tctggattcc gatgatcgac tggtacgccc tggtctttat
      781 cgatacgagc gagctgatca gttttgaccg gtttgctccc ctgatcgcca tcatctcggc
      841 tgccctggtc accctctttt tggccatgct gttcttcacc aacaacaggc taatcaagcc
      901 gattgtccgt ctctcggcca tcatgacacg gatgcagggc ggggatctcg aggtacgggc
      961 cgaggccacc ggccatgacg aaatcggaga actggccctg gcctgcaatg ccatggcgga
     1021 cgaaatcaag ggctacacct cacgtcttga ggagatggtc cggaaacgga ccgaagaact
     1081 cgtgcgcaag aatcacgaga tggttgacag catctcctac gcccgtttga tccagtccgg
     1141 gatgctcccc aagcgcagcc ggatctcgca ccggatctcg gatcacagca tcatctggag
     1201 gccgagggac attgtcagcg gcgatttcta ctggttcaag gaagtcgagg acatactgta
     1261 tctggcggtt gtcgactgca ccgggcatgg tgttcccgga gccttcatga cgatgacggc
     1321 caatgccgtc cttgatcatg tcatctggta cctcaatgat gatcccgccg caatgctgat
     1381 ggaactggac cgcctgatgc ggacgaccct gaaccaggat gatgcatccg atgcccagtc
     1441 caatgacggt ctggacatcg cgctggtgat gatccagccc gaaaaacact cttttgtcta
     1501 ctccggggca cggattcccc tctttttcat gcataaaggc aaagtcgaga tggttcgggg
     1561 ggacagacag agtatcggct ataccaacag cgagcaggtt ctttcgttta ctgcacacga
     1621 gatcaactat gttcccggag accggctctt catctcgacc gacggactct tcgaccagac
     1681 cgggggcgac cgcgccaagc ccttcgggac aacccgcttc agcgaactga tcctcgaaac
     1741 cgccggtctg gacctcgagg ggcaacgcaa ccggattctg atggaactcg acacgcatgc
     1801 cggtggcggg ggtcaactgg atgacatcac ggtctttgca ctggcattgc catagcctcg
     1861 gctgattttg gattcgggga cgggttggtg cgcctgagga ggcatccact ggagagggtt
     1921 ccgcccgccg ggaaagtcgc accatcgcat catgtgtgtg tgtcaggatt ccagcgatgc
     1981 atcccgataa aagttgcatg atggttttgt gtgtttccgg ccggcttccg gcacgccctg
     2041 cccgcaatca gacaatcaca ttccgcaaga atcaggggcc cccggagtgt ggtgcgtccc
     2101 gtgcttgtac ctgaaaacag acccggaacg gatgctagcc ccgacaggat ctcagagcac
     2161 cagcgagacg cgcagaaccg gaccgccaat aacggtcgca ccgcccattg tgttgatgaa
     2221 ttcgatccgg actccggcat ccagtcgcag ccagtcaaca aactcgaaac gcaggagcgc
     2281 cccagcccgg aaggcgagat ccatggtcga ggtatccacc agggtactgc cgagtcctgt
     2341 catcaagag
//
LOCUS       DAOSHE010000351         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44715_length_2345_cov_3.028384, whole genome shotgun sequence.
ACCESSION   DAOSHE010000351 DAOSHE010000000
VERSION     DAOSHE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44715_length_2345_cov_3.028384"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1077)
                     /locus_tag="PLD82_08715"
     CDS             complement(151..1077)
                     /locus_tag="PLD82_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG65500.1"
                     /translation="MAEMELALEDLRAQVKQGREYTLAESFYFNNSILIGTERVLSEK
                     DLDRMDGKCGGFVRVKEYKTAGIENSLVNEVIQLCIKILKEHKDYAGLHSEKRKRIEM
                     TIVNILPGYDYLIMRLAQMRKVIPRIFVHSVNTGIKALLIDNAWQLKYNQGMQNSLRS
                     EEILVGSILRNIGFLRTSKEVVAAKIGELRLARNPLYLKVPDFSAEIIEKDRDKHEID
                     QNILTIVRQSFEYADGTGFPKSLKGEEIYPLALLVSLAAEFDLLLSGELSQNNRSFPE
                     VLRRLMSIANRFDKAQITIISEEFRYLHSARA"
     gene            1395..1907
                     /locus_tag="PLD82_08720"
     CDS             1395..1907
                     /locus_tag="PLD82_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65501.1"
                     /translation="MKQGIMRVVLGFLVLTGCSIFRGPASAGERSGAGSGGAGAGRTS
                     PAGNRVVETGKRLSFDEKRIFRGSCWDFVTGVYQAAGYPEGKRQVVFRREGNGSYADP
                     GILEPGDWVLHYNLEFGNVDHSSIFIDWIDRERALARTLDHVGMNRPEPGRYREHRMT
                     KIFMIMRARD"
     gene            complement(1908..>2345)
                     /locus_tag="PLD82_08725"
     CDS             complement(1908..>2345)
                     /locus_tag="PLD82_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG65502.1"
                     /translation="DDDDLNAEVFHGDWYLTGDRATIDSDGYFWFVGRSDDVIKSCGY
                     RIGPFEVESALVEHEAVAEAAVIGVPDRMKGQRVKAFVILKKEFEPSPALARRLQEHV
                     LSVTASYKCPKEIEFVTELPKTISGKIRRVELREREAEKHRQS"
BASE COUNT          478 a          603 c          770 g          494 t
ORIGIN      
        1 cggggtgcat ccgtgaccag aatgctgcgg gactctggct ttcaaagtct ttttgtgaac
       61 acgcggccat ccacaaagac gaggttccaa acctggtgga gcgcgcttca ggggagtggt
      121 actccctgga gtaatgcagg gaccggcccc tcaggcacgg gccgagtgaa gatagcggaa
      181 ttcctcactg atgatcgtga tctgggcctt gtcgaagcgg ttggcgatgg acatcagccg
      241 gcggaggact tcggggaagg agcggttgtt ctgggagagt tctcccgaga gcaggagatc
      301 gaattcggcg gcgagcgaaa ccaggagggc cagggggtag atctcctcgc ccttgaggct
      361 tttgggaaaa ccggtcccgt cagcgtactc gaagctctga cggacaatcg tgaggatgtt
      421 ttggtcgatt tcgtgtttgt cccggtcctt ctcgatgatc tccgccgaga aatcggggac
      481 cttgagatag agcgggttgc gggcaaggcg gagttcgccg attttggcgg ccacaacctc
      541 cttgctggtg cgaaggaagc cgatattgcg caggatcgaa ccgacgagga tctcttcaga
      601 gcggaggctg ttttgcatcc cctgattgta tttgagctgc caggcgttgt cgatcaggag
      661 ggccttgatg cccgtgttga cggagtggac aaagattctg gggatcacct tgcgcatctg
      721 ggccagccgc atgatcagat agtcatagcc gggcaggatg ttgacaatcg tcatttcgat
      781 gcgcttgcgc ttttcggaat gcagaccggc gtagtccttg tgctccttga gaatcttgat
      841 gcagagctgg ataacttcgt tgaccagact gttttcgatt ccggcagtct tgtactcctt
      901 gacccggaca aacccgccgc atttcccgtc catccggtcg agatcttttt cggagagcac
      961 ccgctcggtt ccgatcagaa tgctgttgtt gaagtaaaaa ctctcggcga gagtgtactc
     1021 cctcccttgt ttgacctggg cccgaagatc ctcaagtgca agctccatct cggccatacg
     1081 atctccccca tcctgaacac tgccagaatc accatgacag tatagatgga aggagcattc
     1141 aggtcaagac gggtgacgcg ggaaaaagcc gacttgacgg attcattttc caccgggatg
     1201 gaccggaatc gtctgacagg agaggaaact ggtgtgggca gcctgaggag aggctgccgg
     1261 cgggtgcatg gcctgaggct gttcagaaaa aagaaccggg agaggtggca ccggatggag
     1321 aggcaagggc ggaaattctc gcgcttgaca gccggacggc aggccggtag catgagccgg
     1381 agctggaggg tgtcatgaaa caggggatca tgcgggttgt actcgggttt ctggtgttga
     1441 cgggatgcag catctttcgc gggccggcaa gcgccggcga gaggagcggt gcgggaagtg
     1501 gcggagccgg ggctggcaga acaagtccgg ccggcaacag ggtggtcgag actgggaagc
     1561 ggctctcgtt tgacgaaaag aggattttcc gcgggagttg ctgggatttt gtgaccgggg
     1621 tataccaggc cgccggttac ccggaaggaa aacggcaggt ggtttttcgg agggaaggga
     1681 acgggtccta tgcggacccc ggcattctcg agccaggcga ctgggtcctg cattacaatc
     1741 tcgagttcgg gaatgttgac cacagttcga tctttatcga ctggatcgac agggagcggg
     1801 cgctggcccg gacgctggac catgtcggga tgaaccggcc ggagcccggg cgctaccggg
     1861 aacaccgcat gaccaaaata ttcatgatca tgcgggccag ggattgatca ggactgcctg
     1921 tgtttttcgg cttcccgttc acggagctcg acgcggcgga tctttccgct gatcgtcttg
     1981 ggaagttccg tgacgaattc gatttccttc ggacatttgt acgaggcggt caccgagagg
     2041 acatgctcct ggagacggcg tgccagggcg ggtgagggct cgaattcctt cttcaggatg
     2101 acaaaggcct tgacacgttg gcccttcatc cggtcgggga caccgatcac ggccgcttcc
     2161 gcaacggcct cgtgctcgac cagggcgctc tcgacctcga agggaccgat ccggtagcca
     2221 cagctcttga tgacgtcatc cgagcggccg acaaaccaga aatagccgtc ggagtcgatt
     2281 gtggcccggt ctccggtcag gtaccagtcc ccatggaaga cttcggcatt caggtcgtca
     2341 tcatc
//
LOCUS       DAOSHE010000352         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_44844_length_2341_cov_4.403325, whole genome shotgun sequence.
ACCESSION   DAOSHE010000352 DAOSHE010000000
VERSION     DAOSHE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44844_length_2341_cov_4.403325"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLD82_08730"
     CDS             complement(<1..645)
                     /locus_tag="PLD82_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG65503.1"
                     /translation="MSLVITSTANPRIKAVAALSQPRERRLSGLCTIEGHDEVRLARD
                     SGSAIREVYLCPDLMRASDLVFSETLRAAGVDLVEVSEAVMTKMAYREHPDAWLAVAE
                     SPDTDLATFSARLRLRQAQSAARTGKQVDERPRTLLVLDSVEKPGNIGAVLRSADAAG
                     IDGIIASNCRTDMGNPNVVRASKATVFTIPVAECSPDEALGWITHEGITLYAASP"
     gene            703..1743
                     /locus_tag="PLD82_08735"
     CDS             703..1743
                     /locus_tag="PLD82_08735"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG65504.1"
                     /translation="MDKATDGVTLSRVLETDFCGQRLDGVLRLLRPDWSRSIVQGFFT
                     DGLVLLNGRPARAAQRLRGGERLEILHAATSAISPPHLSVLYEDAVLLAVDKPSGIPS
                     HPLRAGETGTALDGMALLSPAVFFYGEQSSGQGMGAGGEVSVEARQRSLEGGLVHRLD
                     NDTSGVLLAAKSEADRQALRAALEDPAAYKEYLAIVHGQLEKECDIGFPVAHAAGDAR
                     RSVAVFPQGWPIPPGAARVRGRFRGRPRSALSHVVPLECVADCTLVRVRVHKAVTHQV
                     RVHTAALGHPIAGDPRYLPAGWPAHAPRLALHASLIAFRHPGSGEMLHIAAPWAPDLA
                     QAWREMGGRFSG"
     gene            complement(1810..>2341)
                     /locus_tag="PLD82_08740"
     CDS             complement(1810..>2341)
                     /locus_tag="PLD82_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG65505.1"
                     /translation="MTRLDMSAAELMPRIRTSSIFRYLSDGECLDLVNKGIVTRYEAK
                     ERFITEGEESPTFYVVVGGVVNITVHERTGKEVFICAIGEGDVIGEAAIFMNRPRTAN
                     AITAEPTILLEFTRDRLLGFLRNNPTAGLKILMIVIHSLLNKLRDANQELAFERKAAY
                     KQSEVDDLINSYFGNQ"
BASE COUNT          401 a          667 c          774 g          499 t
ORIGIN      
        1 gggggaggcc gcgtagaggg tgatgccttc atgtgtgatc caacccaggg cctcgtcggg
       61 gctgcattcg gcgaccggaa tcgtaaaaac ggtcgccttg ctggcgcgga ccacattcgg
      121 gttgcccatg tctgtccggc aatttgatgc gatgatcccg tcgattccgg ccgcatcagc
      181 cgagcgcagg acggccccga tatttcccgg cttttcgacg gaatcaagaa cgaggagggt
      241 gcgtggtcgt tcatccacct gtttgcccgt tcgcgcagcg gactgggcct gacgcaatct
      301 gagtcgggct gaaaaggttg ccagatcagt atcgggactt tcggcgaccg ccagccaggc
      361 atcaggatgt tccctgtagg ccatctttgt catgacggcc tcactgactt cgacgagatc
      421 gactccggct gctcgcagcg tttcgctgaa caccagatct gaggcacgca tcaagtccgg
      481 gcaaaggtag acttcacgga tggcgcttcc cgagtcgcgt gccagccgga cttcgtcgtg
      541 gccctcgatg gtgcagagcc cgctcagacg gcgctcacgc ggttgggaca aggctgcgac
      601 tgccttgatc cggggattgg ccgtgctggt gatcacgagc gacatcctgc ctccagtgca
      661 gcccgttgcg ccgggctggc ctttgtgtgt accttcaata atatggacaa agcgacggat
      721 ggtgtcactc tctcccgtgt tctcgagacg gatttttgcg ggcagcgcct ggatggggtg
      781 ctgcgcctgc tccgtccgga ctggtcgcgc agcattgtgc aaggtttttt tacggatggt
      841 ttggtcctgc tcaatggtcg gcctgcacgg gcggcgcagc ggttacgggg tggcgaacgg
      901 cttgaaatcc tgcatgccgc caccagtgca atttcgcctc cacacctgag cgttctgtat
      961 gaggatgcag tattgcttgc ggtcgacaaa ccctcaggga ttccttccca tcccctgcgt
     1021 gctggcgaga cgggaacggc tcttgacggg atggcactgc tctctcctgc tgtttttttc
     1081 tatggggaac agtcctcagg ccaggggatg ggcgcgggtg gcgaagtatc agtggaggcc
     1141 aggcagcgca gcctcgaggg agggctcgtg caccgcctcg acaatgatac ctcgggtgtg
     1201 cttctggccg ccaagagcga ggcggaccgg caggccctgc gcgctgccct cgaagacccg
     1261 gcagcgtaca aggagtatct ggccatcgtg cacggacagc ttgaaaaaga atgcgatatc
     1321 ggttttccgg tagcgcatgc ggcgggggat gcacgccgga gtgtcgcagt ctttcctcag
     1381 gggtggccca taccgccggg ggccgcccgg gtcaggggcc gttttcgcgg gaggccgcgc
     1441 tctgccctga gtcatgttgt tccattggag tgtgttgcgg actgcaccct ggtccgggtc
     1501 cgtgtccaca aggcggtcac gcatcaggtc cgggtgcaca cagccgcgct tggccatccg
     1561 atcgcaggtg acccgcgcta tcttccggcg ggctggccgg cgcacgcgcc acgcctggcc
     1621 ctgcatgcct cgctgattgc gtttcggcat ccgggatcag gggagatgct gcacatcgct
     1681 gctccgtggg cgcctgacct ggcgcaggct tggcgggaga tgggcggacg tttttcgggg
     1741 taaaaccagt gtgcacaata tgcttctgag agccgaatcc cggaggcctg aggctgttca
     1801 tcaaaaaaat cattgattcc caaagtagga attgatcaaa tcgtccactt cactctgttt
     1861 gtaggcggcc ttgcgctcaa aggccagttc ctggttggcg tcccggagtt tgttgaggag
     1921 gctgtggatc acgatcatca agattttgag gccggcggtg gggttgttgc gcaaaaaacc
     1981 gagcaggcgg tcgcgggtaa actcgagcag gatggttggc tcggcggtga tggcgttggc
     2041 cgtgcgcggg cggttcatga agatcgccgc ctcgccgatg acgtcaccct cgccgatggc
     2101 gcagatgaag acttccttgc cggtgcgttc gtgaaccgtg atgttgacga caccgccgac
     2161 gaccacgtaa aaggtcgggc tctcctcgcc ttcggtaatg aagcgttcct tggcttcgta
     2221 gcgggtgacg atccccttgt tgacaagatc gagacactcg ccgtccgaga gatagcggaa
     2281 aatgctggat gtgcggatgc gcggcatcaa ttcggcggcg ctcatgtcca gtcgggtcat
     2341 g
//
LOCUS       DAOSHE010000353         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45046_length_2334_cov_4.029399, whole genome shotgun sequence.
ACCESSION   DAOSHE010000353 DAOSHE010000000
VERSION     DAOSHE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45046_length_2334_cov_4.029399"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PLD82_08745"
     CDS             complement(<1..1512)
                     /locus_tag="PLD82_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65506.1"
                     /translation="MSPRLFILTWILLTPLTLFAEERPELNTVTGKEFVRLTRLRISP
                     GLFERIRSELGGFENPAQLLLLFHVPESRHIRLTNAFICTKTFHAINDVSDNDEEAQE
                     EEAAPEPIPLAGCTPSRLEAIPGMTPLMAGRIAHACRQPGLYERWQLLAVRGMTAALY
                     GRLRRFLQARRTTLDNPYRGMLRAGARNTGEAWRLPLALSLESEGFELRMGARGETGL
                     AERRMSGIFGIGTRLPRPVTELSGYLRLAPSDWNLLIGQYRLRSPLGLLSGKQRSAGD
                     LSLETDSMPGYIPVSGTRTPEVTSGLLGEYQAGRWTFSASAGLMAWPLREEALSNDRI
                     TSSLSSLVTGLGDIPIHHLRLGLGASWLPADWGRVSALWIRESFDHGFLPNTRRPEFT
                     SSIRDRVLLSTGVQIGACLISGEFITAFEGGKPSSLKTRGGAGGISLADGDTRAGFVF
                     YDRSRIHPGLLDGLASDLTANRGILLEGRTRLDETLGIEGTLAILGRENVGWKT"
     gene            complement(1509..>2334)
                     /locus_tag="PLD82_08750"
     CDS             complement(1509..>2334)
                     /locus_tag="PLD82_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG65507.1"
                     /translation="LACDLGIRIVPMEGLSLGVAVYAFNQPEFEAGEHMSTRMRAGLE
                     FPVAASWTIRAGIQADEWGQETALSASWIPVPGLEILTTCEPGTPALALGLSLSAGEF
                     EFALANRLGLASHLLTHALSISISPSREETQPWKGRVNLNTASLQTLLSLPGMTRRMA
                     TRIIRERNERPFASVQDLVRVHGITRRVLLPWMARSSVMESGTNLHLLTNASGQTSWI
                     NGMEIRHLMARGIRADLARRIILLRTSLHGFFDPADLALLPGETTNWIPLVFGDPR"
BASE COUNT          452 a          683 c          765 g          434 t
ORIGIN      
        1 ggttttccac ccgacatttt cccgaccgag gatggccagc gtgccttcga tcccgagagt
       61 ctcgtcgagt cgggttcgcc cttcaagaag gatcccccgg ttggctgtca ggtccgaagc
      121 cagcccgtcc aggagtccgg ggtggatccg gctccggtcg tagaacacga aaccggcacg
      181 ggtatcccca tcggccagcg agattccgcc ggctccaccc cttgttttga gggaggaggg
      241 tttgccaccc tcgaaggcgg tgataaattc gccggaaatg aggcaggctc cgatctgcac
      301 acccgtagac aggagcacgc ggtcgcgtat cgacgatgtg aattccggcc tgcgggtgtt
      361 cggcaaaaaa ccatggtcaa aggattcacg gatccagagg gcagacaccc tgccccagtc
      421 cgcgggcagc caactcgcac ccaggcccag tcggagatgg tggatcggga tgtcacccag
      481 gccggtcaca agggatgaga gactcgaggt gatccggtca ttcgagagtg cttcctccct
      541 gagtggccag gccatcaggc cggccgaggc cgaaaacgtc catcgtccgg cttgatactc
      601 gccgaggaga ccactggtca cctcgggcgt gcgcgttccg ctgacgggga tgtagcccgg
      661 catggagtct gtttccaggc tgagatcgcc ggccgagcgc tgttttcccg aaaggagacc
      721 gagaggtgag cggagccggt actggccgat gagaagattc cagtccgacg gtgcaagacg
      781 gagatagccg gagagctccg tcactggccg gggaagccgg gtcccgatcc cgaagatgcc
      841 agacatgcgc ctctcggcaa gtccggtttc tccccgtgca cccatgcgga gttcaaaacc
      901 ttcgctctcg agagacaggg caagagggag gcgccatgcc tctcccgtgt tacgggcccc
      961 cgcgcgcagc atcccccggt acggattgtc gagcgttgtg cgccgggctt gcagaaagcg
     1021 tctcagtcgg ccatagagag ctgccgtcat cccgcggacg gcgaggagtt gccagcgttc
     1081 gtacaacccc ggttgtcggc aggcatgggc gatccggccc gccatgagtg gtgtcatccc
     1141 cgggatcgct tccagccggc ttggtgtgca gcccgcgagg gggatcggct ccggggcggc
     1201 ttcctcctcc tgcgcctctt catcgttgtc cgaaacgtca ttgatggcat ggaaggtctt
     1261 tgtacagatg aaggcattcg taagacggat atgacgggat tccgggacgt gaaacaggag
     1321 gaggagttgc gcgggatttt caaaaccacc cagttcgctc cggatacgtt caaagagtcc
     1381 gggactgatc ctgagacggg tgagccgcac gaattctttt cccgtgaccg tgttcagttc
     1441 cgggcgttct tcggcaaaga gcgtgagcgg agtcaggaga atccaggtca ggatgaacaa
     1501 cctcgggctc atcgggggtc tccgaagacc aggggaatcc agtttgtcgt ttctcccgga
     1561 aggagtgcca gatcggccgg atcaaaaaaa ccgtgcagac ttgtacgcag gaggatgatc
     1621 cggcgggcca gatcggcgcg gatcccgcgt gccatgagat ggcgaatctc catcccgttg
     1681 atccagcttg tctgcccgga ggcattggtc aggagatgga gattggttcc cgattccatg
     1741 acagacgaac gcgccatcca ggggagcagg actcgccggg tgattccgtg gacccggaca
     1801 agatcctgga cactggcaaa ggggcgctcg ttccgttccc ggatgatccg cgtggccatg
     1861 cgtctcgtca ttcccggaag tgagagcagg gtttggaggg aggccgtgtt caggttgacc
     1921 cgacccttcc aaggctgcgt ttcttcacga gatggcgaga tactgatgga aagcgcgtgt
     1981 gtcaggagat gtgacgccaa tccaagccgg ttggcaagtg caaattcgaa ttcaccggca
     2041 gagaggctca gtccgagtgc cagggccggt gtgccgggct cgcaggtggt gaggatctcc
     2101 agccccggga ccgggatcca gcttgctgag agggcggtct cctgtcccca ctcgtccgct
     2161 tggatgccgg cccggatcgt ccacgaggcg gcgaccggga attcaagtcc ggcccgcatc
     2221 cgtgtcgaca tgtgttctcc cgcttcgaat tccggttgat tgaaggcata cacggccaca
     2281 ccgagggaga gtccctccat tggaacgatc cggattccca gatcgcaggc gagc
//
LOCUS       DAOSHE010000354         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45114_length_2332_cov_3.281072, whole genome shotgun sequence.
ACCESSION   DAOSHE010000354 DAOSHE010000000
VERSION     DAOSHE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45114_length_2332_cov_3.281072"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLD82_08755"
     CDS             <1..790
                     /locus_tag="PLD82_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG65508.1"
                     /translation="DATGRIDLAAFERLVSEKTALVSVMLANNEIGTTQDLAAISAIA
                     HKAGAFVHTDAVQAVGKIPVDVAALGVDYLSASGHKIHGPKGIGIHWMRRGVPFAPLM
                     HGGHQEQGRRAGTVNAPAIAALGTACRIAREQLAKEAARLAGLRDRLRAGLEERITDT
                     TLNGNPDFCLPNTLNMSFRAAEGESTLLYLDLENIAVSTGSACASGSLEPSHVLLACG
                     RSAELAHGSIRFSLGRTTSDEDIEYVLDKLPPIMARIRSMSTLR"
     gene            839..1465
                     /locus_tag="PLD82_08760"
     CDS             839..1465
                     /locus_tag="PLD82_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOG65509.1"
                     /translation="MAENTSSWAYSDRVKEHFMNPRNVLSDEENFQDDGRGTVGSIQC
                     GDEMLVLIKVADGRITDCRWRTYGCASAIASTSALSEMVKGMSLEDAAQITPKQILNY
                     LHGLPDSKVHCSVLGDKALRAAIDDYRQRNGIVVEEKERPARIICDCIGVTDRDIMAA
                     AKDGAVTWNELQQKTKIGTVCGKCKTHAMEVLDESLFEIHHETLKMHE"
     gene            complement(1688..2083)
                     /locus_tag="PLD82_08765"
     CDS             complement(1688..2083)
                     /locus_tag="PLD82_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOG65510.1"
                     /translation="MTVVDTSAWIDYFRGLDAPHTRAVDRALQTDRVVVGDLILAEFL
                     QGFRADKDFEKAADLMNRLEYHDFVGREVALAAAVIYRSLRKRGVTVRKTIDMLIGTW
                     CLMHGCPLIHNDRDFDPLEKHFGLIVTKA"
     gene            complement(2080..2277)
                     /locus_tag="PLD82_08770"
     CDS             complement(2080..2277)
                     /locus_tag="PLD82_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOG65511.1"
                     /translation="MRTNIIIDDRLMDEALAVSECRTKKETVDEALRLFVAMKRQEAI
                     RASRGTLHWEGSLDQMRTDRV"
BASE COUNT          518 a          709 c          633 g          472 t
ORIGIN      
        1 tgatgcaacc ggcaggattg atctggccgc gtttgaacgt cttgtcagcg aaaaaacggc
       61 tctcgtttcc gtcatgctgg ccaataacga aatcggcaca acccaggacc ttgcggccat
      121 ctcggctatc gcccacaagg ccggagcctt tgtccacacg gacgcggtcc aggccgtggg
      181 caagatcccg gtggatgtcg ccgcactcgg ggtggactat ctttcggctt ccggccacaa
      241 gatccacgga cccaagggaa tcgggattca ctggatgcgg cgcggggtcc cctttgcacc
      301 cctgatgcat ggcggccacc aggaacaggg ccggcgcgcg ggcacggtca acgcaccggc
      361 catcgccgca ctgggaactg cctgccggat tgcccgggaa cagcttgcga aggaagctgc
      421 ccgccttgcc ggattgcgtg atcgcctgcg cgccggcctg gaagagcgca tcaccgacac
      481 cactctcaac ggaaacccgg acttctgcct tccgaacacg ctcaacatga gtttccgggc
      541 cgctgagggt gagtctaccc tgctctatct cgatctcgaa aacattgcgg tctcgaccgg
      601 ctcggcctgt gcatccggtt cgctcgagcc gtcccatgtt ttgttggcat gcgggcgctc
      661 ggccgagctg gcccacggct caatccgctt cagtctgggg cgcaccacca gcgacgaaga
      721 catcgagtat gttctcgaca agctcccgcc catcatggcc cgcatccgct cgatgtccac
      781 cctgcgctag aaaccgaatt tcatccgcct gagactgttc acaaaaaagg agaaaaccat
      841 ggccgaaaac acatcaagct gggcctattc agaccgtgtc aaggaacact tcatgaaccc
      901 gcgcaacgtc ctgagcgatg aagagaattt ccaggatgac ggcaggggga ccgtgggcag
      961 catccagtgt ggcgacgaga tgctggtcct gatcaaggtc gctgacggcc ggatcaccga
     1021 ctgtcgctgg cggacctatg gttgcgcgag cgcgatcgcc agcacctcgg ccctgtcgga
     1081 aatggtcaaa ggaatgtcgc ttgaagacgc cgcacaaatc acgcccaagc agattctgaa
     1141 ttacctgcac gggctccccg actccaaggt ccactgctcg gttctgggcg acaaggcgct
     1201 gcgtgccgcg atcgatgatt atcgccagcg caacgggatc gttgttgaag aaaaagaacg
     1261 cccggccagg atcatctgtg actgcatcgg cgtgacggat cgggatatca tggccgcagc
     1321 caaagacggc gcggtcacat ggaacgaact gcagcaaaag acaaaaatcg ggaccgtctg
     1381 cgggaagtgc aagacccacg ccatggaagt cctggacgag agtctatttg aaatccatca
     1441 cgaaacgctc aagatgcacg aatagctttc gcttttcatg cccgcaggct cgagcctgcg
     1501 ggtttatgac gcactgcccg atcaaaccac gatcatcttt cggtcttttt tttctgtgaa
     1561 cattctcagg ccggactgag tcgggattta ttcgaagggt gtgcatcccg tcttatatac
     1621 ttctctctga gtctcagatt gcaagaggac tcactgaagg gaaggccttc gatcaatacc
     1681 ccctacatca ggctttggtg acaatcagac cgaagtgttt ctcaagcgga tcaaagtcgc
     1741 ggtcgttatg gatcagcgga cagccatgca tcaggcacca ggtcccgatc agcatgtcga
     1801 tcgtttttcg gaccgtgacc ccacgcttcc gcagggaccg atagatcaca gcagccgcca
     1861 gagcaacctc gcgtccgaca aaatcatgat attccaaacg attcatcagg tcggcggctt
     1921 tctcgaaatc cttgtccgca cgaaaaccct gtaaaaactc ggcaaggatc agatcaccca
     1981 cgaccacccg gtccgtctgc aaggctctgt cgacagccct ggtatgcggc gcgtcaaggc
     2041 cgcgaaaata atctatccag gctgatgtat caacaacggt catacccggt cagtccgcat
     2101 ctgatcgagg ctgccttccc aatgcaacgt cccccgactg gcccgaatgg cctcctgtcg
     2161 tttcatggcc acaaagagtc gcagggcctc gtcgaccgtc tcttttttgg tccggcattc
     2221 agacacggcc agcgcttcat ccatcagcct gtcatcaatg atgatattgg tccgcatacg
     2281 cagctccttg tgtgtatctt tagttcattt tatacacata gaggggcgtt gt
//
LOCUS       DAOSHE010000355         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45221_length_2328_cov_4.561813, whole genome shotgun sequence.
ACCESSION   DAOSHE010000355 DAOSHE010000000
VERSION     DAOSHE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45221_length_2328_cov_4.561813"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2194
                     /locus_tag="PLD82_08775"
     CDS             <1..2194
                     /locus_tag="PLD82_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HOG65512.1"
                     /translation="SGKKYFTAMTAIEGIVTAKKAVPDRILIVQDVSASMAGRDKDRE
                     WALLRRWLRTVKPGAEIRLVQFSHAVHNEKTYRNEGDLASLRQAIDDAAFDGGTSMGA
                     VDLSKYQADVCIFLTDGISNFGPDELKTARYPVYAIASQQTGEHAWLRQITGSTGGEY
                     VNLARMTDDQAYDRLSGVSAHILSVTGRGIRDIHPGPGAALSSIFTFAGILEGDEGEL
                     VVRYGTAQAFKEVRIPIKASTSLTTTTNGIVRRIWAQKRIADLETRWSKNETEIIKLA
                     KTFGIVTRGTSLIVLDRIEDYVRYRIVPPTELHAEYYNAIAELDKQKQKIEADHIEDV
                     VRRFALMQAWYDKSYPKDTPARRDDTKKLAGESTGGERNGTAARPSVAMRDGMAEESD
                     RVTDAHPRAERERGLDSAAKRDSSGNAGAEGAIELKKWTPDVPYLKDLEKADKDKRYA
                     VYLGLRKQWANSSAFFLDVADFFAEQGDKATALRVLSNIAEMDLENPQLLRILGHRLA
                     QLGWLDLSEKVFADVLAMKPEDPQSYRDLGLVLDRAGKSQRAVELLYQVATKKWDSRF
                     PDIELIALVEMNGIISRGAKVDTSKLDRRVIRAMPSDVRVVLNWDADNCDMDLWVTDP
                     NDEKCYYANKLTYQGGRMSMDFTRGYGPEEYLVRLAKSGKYKIEVNYYGNSQQTIAGA
                     TTVQLQLFLNWGRPNQQVKEITLRLKDNKEVIEVGTFAISGGTITPVK"
BASE COUNT          512 a          693 c          678 g          445 t
ORIGIN      
        1 ttcaggaaaa aaatacttta ccgccatgac cgctatcgaa gggatcgtaa cggccaaaaa
       61 agcggttccg gaccggatct tgatcgtcca ggatgtctcc gcatccatgg ccggccgcga
      121 caaggaccgt gagtgggcat tgcttcgccg ctggttgaga accgtcaagc ccggcgccga
      181 gatccggctc gtccagttca gtcatgccgt ccacaacgag aaaacatatc gcaacgaggg
      241 tgatcttgcg tctctccgtc aggccattga tgatgccgcc tttgacggtg gtacaagcat
      301 gggtgccgtc gatctctcaa agtaccaggc cgatgtctgc atcttcctca cggacgggat
      361 ctccaatttc gggccggatg aactcaagac cgcacgctat cccgtctacg ccatcgcttc
      421 acagcagacc ggtgagcacg cctggttgcg ccagattacc gggtcgaccg gtggcgaata
      481 cgtcaatctt gccaggatga ccgacgacca ggcctatgac cgtttgagcg gcgtgtcggc
      541 gcatatcctt tcggtgaccg gccgcgggat cagggatatc catcctggtc cgggcgccgc
      601 cctctcgtcc attttcacct tcgccgggat tcttgagggt gacgaaggcg aactcgtcgt
      661 gcgctacggg acagcccagg cattcaaaga agtccgcatc ccgatcaaag cttcaacctc
      721 tctgacgacg accaccaacg ggatcgtgcg ccggatctgg gcccagaagc ggatcgccga
      781 tcttgagacc cgctggagca agaacgaaac cgagatcatc aagctggcca agacctttgg
      841 tatcgtcacc cggggaacct ccctgatcgt tctcgaccgg atcgaggatt acgtccgcta
      901 ccggatcgtt ccaccgacgg agcttcatgc cgagtattac aacgccatcg cggaactcga
      961 caagcagaag cagaagatcg aggccgacca tattgaagac gtcgtccgcc gtttcgccct
     1021 catgcaggcc tggtatgaca agagctatcc caaggacacc cccgcccggc gtgatgacac
     1081 caaaaagctg gctggtgagt caactggtgg tgagcggaac ggaacggctg cacgaccgtc
     1141 cgtggccatg cgcgacggaa tggctgaaga gagtgaccgt gtgactgatg cccatccccg
     1201 ggccgagcgc gagcgcggac tggacagtgc ggccaaacgt gattcttcgg ggaatgccgg
     1261 agccgagggc gcgattgagc tgaaaaaatg gacacccgat gttccctacc tcaaggatct
     1321 ggaaaaagcc gacaaggaca agcgctatgc cgtctatctt ggcctgcgca aacagtgggc
     1381 caacagttcc gcattcttcc tggatgtcgc ggacttcttc gcggagcagg gtgacaaagc
     1441 gacggctttg cgtgtgcttt ccaacatcgc cgagatggat ctcgagaatc cccagctcct
     1501 gcgcatcctt ggtcatcgcc tggcccagct tggctggctt gacctgtccg aaaaagtttt
     1561 tgcggatgtc ctcgccatga aacccgagga tccccagtcg taccgtgatc tcgggcttgt
     1621 ccttgacagg gcgggcaaat cccagcgcgc tgtcgaactc ctctatcagg ttgcgacaaa
     1681 aaaatgggac agccgcttcc ccgatatcga gctgattgcc ctggtcgaga tgaacgggat
     1741 catctcgcgt ggtgccaagg tggatacgtc aaagctggac aggcgggtta tccgtgccat
     1801 gccctcggat gtccgggtcg tgctcaactg ggatgcggac aactgcgaca tggacctttg
     1861 ggtgaccgac cccaacgacg agaagtgtta ctatgccaac aagctgacct accagggcgg
     1921 acggatgtcg atggacttta cccgcggcta tggacccgag gaatatcttg tgcgtctggc
     1981 caagtccggc aagtacaaga tcgaggtcaa ctactacggc aattcgcagc agaccatcgc
     2041 cggtgccacg accgtccagt tgcagctctt cctcaactgg ggccgcccga accagcaggt
     2101 caaggaaatc accctcagac tcaaggacaa taaagaggtc atcgaggtgg gcacctttgc
     2161 catcagcggg gggacaatca cccccgtcaa gtgatgtttg atgaacaatc ggccgcggac
     2221 gtgacgcggc tatccacccg gtgccggcct tgcccgcacc gggtttttgt attgagggcg
     2281 aagtcctcgc agagggcgaa gtcctcgcag agggcgaagt cctcgcag
//
LOCUS       DAOSHE010000356         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45228_length_2328_cov_3.605807, whole genome shotgun sequence.
ACCESSION   DAOSHE010000356 DAOSHE010000000
VERSION     DAOSHE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45228_length_2328_cov_3.605807"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLD82_08780"
     CDS             complement(<1..1053)
                     /locus_tag="PLD82_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65513.1"
                     /translation="MRLWTLYASLILTGCAILACSSGTGLKAERPDDGGYALLSARLQ
                     ALVRDGYYREAAEIGKKIIPLAEARFGTRHEETLRSMTLAGLALRKNGEYAEAEAILL
                     AANSNAMALWGKKHVGLVEIRNSMAELYLAYGKMDAAAKYADQAWTGAEDWRTIDPLK
                     YAEALKTAGLVQYHLGEYDRALAFYKSAYQIRLTNLGANHLLTAESLNNLAVVHTLKG
                     DYTNGLAKYQRSLAIRTNLLSPGHPDIAQSWNNLASFHFFHGAYDEALQYYLKAYELW
                     KERLGLLHPETGTCLNNLAEIYKRQKNWDKAEEYYKQAIKIFEATPGRQHFLLSTVLH
                     NLATLYDTQGNLASAEH"
     gene            1234..1935
                     /locus_tag="PLD82_08785"
     CDS             1234..1935
                     /locus_tag="PLD82_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65514.1"
                     /translation="MKKTLTVLGAALALLAGCGSSEDPALVARIKSMSEQKSRTMQAS
                     GQIAAPAYRAGQYVTMVTIEGKKRSITQTGIVGQRDGGWVIEFSSITPSEETVSQICF
                     AGLEKLAQGAGTDALEIKWIKIKDADGKVSVMSRENNPFFGMMTGSYKDMAAGMQFKA
                     VTTYEQGGTVSVPAGVFNGTLKVNSTVTVMGNEYKSVNWMHSGVPINALVRSVADDTV
                     LELVDYGYDYKAKLD"
     gene            2093..>2328
                     /locus_tag="PLD82_08790"
     CDS             2093..>2328
                     /locus_tag="PLD82_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOG65515.1"
                     /translation="MPKELMSGNEAFAAGAWAAGVRVGAGYPGTPSTEILENLKNYPD
                     IASEWSVNEKVALEVGIGASLGGARTLVTMKHVGL"
BASE COUNT          509 a          589 c          677 g          553 t
ORIGIN      
        1 atgttccgcc gaggcgagat tgccctgtgt gtcataaagt gtagccagat tgtgcagaac
       61 ggtcgaaagg aggaagtgct gtctccccgg agtcgcttcg aatatcttga tggcctgttt
      121 gtaatattcc tcggctttgt cccagttttt ttgccgtttg tagatctcgg cgaggttgtt
      181 gagacaggtt ccggtttcgg gatgcagcaa tcccagccgt tccttccaga gttcataggc
      241 cttgaggtag tattggaggg cttcgtcata cgcaccatga aaaaaatgga agctggccag
      301 attgttccat gactgtgcga tgtcgggatg cccgggactg agaagattgg tccggatggc
      361 gagggatcgc tggtattttg ccagtccgtt ggtataatcc cctttgagcg tatggacgac
      421 cgcgagattg ttgagggatt cagccgtcag aagatgattg gcgccaagat ttgtcagtcg
      481 gatttggtaa gcagatttgt agaaagccag ggccctgtcg tactcgccca ggtggtactg
      541 gaccagcccg gctgttttca gggcttcggc gtatttgagg ggatcgatgg ttctccagtc
      601 ttcggctcct gtccatgctt ggtcggcata ttttgcggct gcatccattt tcccgtaggc
      661 gagatagagt tcggccatac tgttccggat ttccaccaag ccgacatgtt ttttgcccca
      721 aagtgccatg gcattgctgt tggcggcaag caagatcgcc tcggcttcgg catattcacc
      781 gttttttctc agggccaggc cggccagcgt catggaacgc aaggtctcct catggcgcgt
      841 cccgaagcgg gcctcggcaa ggggaataat ttttttcccg atctcggccg cctcacggta
      901 atacccgtca cgaaccagag cctgcaaacg tgccgagagg agggcatagc cgccatcgtc
      961 cgggcgctcc gctttgagcc cggtcccgct actgcaggcg aggatggcac aaccggtcag
     1021 tatcaggctt gcatagagtg tccagagacg catgggattc ccccgacgag tagtctgtct
     1081 cttgtatcgg caatgggtag ccggtttctt cattaaatga cggaaaaaca ggtgtcgagc
     1141 gatgaaatcg ggagtcccgg ccatggatgt ttgtgtattt ttcaaagaga tgccggtgtg
     1201 cgaaacaccg gaaaccgaga ggagacacaa attatgaaaa aaactctgac agtgttgggt
     1261 gctgcgctgg cactcctggc cggatgcggt tcgtccgagg atccggctct ggtcgcccgg
     1321 atcaagtcca tgtccgaaca aaaatcccgg accatgcagg cttcgggcca gattgccgct
     1381 ccggcataca gggccggaca gtatgtgacc atggtcacca tcgaggggaa gaaacgttcg
     1441 atcacacaga ccgggattgt cggtcagcgt gatggaggat gggtaatcga attctcttcc
     1501 atcacaccca gcgaggaaac cgtcagccag atttgttttg ccggtcttga aaaactggca
     1561 cagggtgccg gtacggatgc cctcgaaatc aaatggatca aaatcaagga cgctgacggg
     1621 aaagtgtcgg tcatgagtcg tgaaaacaac ccttttttcg ggatgatgac cggatcctat
     1681 aaggacatgg cggcaggaat gcaattcaaa gccgtaacaa cctatgagca gggcggaacg
     1741 gtctcggtac cggcgggagt tttcaacggg acactcaagg tcaattcgac ggtgaccgtc
     1801 atggggaacg agtacaagtc cgtcaactgg atgcactccg gtgtgcccat caatgcactc
     1861 gtccgttccg ttgccgatga cacagtgctc gaacttgtcg attacggcta tgattacaag
     1921 gcgaaactgg actgagggtt tttgaacagg ccaacgctcc ctggctcgtc cagtggggcg
     1981 tttttttgtg agttctcttc ctgtcatgat cttccagcca gctcattgac ccgtaccggc
     2041 ttcctgcata cttttatcac gtgcatcacc aattcacgtg caggaacaac tcatgccaaa
     2101 agaactgatg tcgggcaacg aggcttttgc ggccggtgcc tgggccgccg gagtgcgggt
     2161 tggagcaggg tacccgggta ctccatcaac cgagatcctt gaaaatctca aaaactatcc
     2221 tgatattgcc tctgaatggt cggtcaacga gaaggtggct ctcgaagtcg ggattggtgc
     2281 atcactcggt ggtgcccgga cccttgtcac catgaaacac gtcggcct
//
LOCUS       DAOSHE010000357         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45314_length_2324_cov_3.891141, whole genome shotgun sequence.
ACCESSION   DAOSHE010000357 DAOSHE010000000
VERSION     DAOSHE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45314_length_2324_cov_3.891141"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLD82_08795"
     CDS             complement(<1..357)
                     /locus_tag="PLD82_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG65516.1"
                     /translation="MSRIVLGLIVLVTMVTACSTEKTGGTETNKGLVAGGRAEVTAAS
                     GLRMRAKPDQKAAIVDVLSSGAILSVLDTKGPEAVIEGKKAHWVKVRYGASEGWVFGG
                     MIRPAEAMKVVGGNGTN"
     gene            complement(628..1392)
                     /locus_tag="PLD82_08800"
     CDS             complement(628..1392)
                     /locus_tag="PLD82_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG65517.1"
                     /translation="MAWYKELFEDYAKQYDTEDFVQHTESEVAFIEEVLAHDTSIRIL
                     DIGCGTGRHSIELAARGYPVTGVDLSKAQLKRARGKAALRNVSVSFEEGDARRLPFEN
                     CFGCALSLCEGGFPLMETDEENFEILKSAARALVPGGILILTTLNGLFPMAHSIKALI
                     NDTSTTSLRVEESSFDLMTFREAETFTVTQDSGRELTITSSERFYVPPELRWLLVTAG
                     FADVELYGCMPGQYNRERKPNPDDFEMMAIARKAVL"
     gene            1696..>2324
                     /locus_tag="PLD82_08805"
     CDS             1696..>2324
                     /locus_tag="PLD82_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65518.1"
                     /translation="MEDDPKLPTSSCAVREFSMQALPRCPIVSTLPSGVVVHDAQGAI
                     LMANPAAEEILGVSLSQMQGKTSLDPIWHCIHEDGSEYPGETHPGMVVLTTRQPMYGV
                     IMGVFHPQKTDYIWIEINALPILSPDGYLNGAYATFTDITARIRHEASVREGWLAMDA
                     IHDLVAVIDSDHRYRVVNQAFLAQRQATNQDIIGKHVREVLGEDVYERD"
BASE COUNT          502 a          762 c          557 g          503 t
ORIGIN      
        1 gtttgttcca tttcccccaa caactttcat cgcctcggcc ggacggatca tgccaccaaa
       61 cacccacccc tcactggcac cgtagcgcac cttgacccaa tgggccttct tcccctcgat
      121 gaccgcttcc ggccctttcg tatcgagcac actgagtatg gccccgcttg acagaacgtc
      181 tacgatcgct gccttctggt ccggtttggc ccgcatccgc aaaccggagg ccgccgtcac
      241 ctcggccctg ccgcccgcca ccaggccctt gttcgtctcg gttccgcctg tcttttccgt
      301 ggaacacgcc gtcaccatag tgaccagcac aatcaaaccc aaaacgattc tcgacatgac
      361 accctcttca cgatggaatt tttctgttct caagtataca ccgcaggctt tttccgcatc
      421 ccctcttttt gaggaccaga ttatcaccgg cgactatggt gacggtattg tcagccccgc
      481 tgtcatggta ccgttttttc tcaaatttga gaaattttct ccgtgttcca cccaaaaatc
      541 cccgtccgcg gacctacgga atcaccatgg tcataaaatt tccaggtatt cccgctcccc
      601 acccggggac tatggtggca tttttcctta cagtaccgct ttccgggcta tcgccatcat
      661 ctcgaaatca tccgggttgg gcttgcgctc cctgttatac tgccccggca tgcacccata
      721 gagctcgacg tcggcaaatc cggcggtcac aagcaaccaa cgtagctccg gtggcacata
      781 aaatcgctca ctgctcgtga tggtcaactc ccggccggaa tcctgtgtca cggtgaatgt
      841 ctccgcttca cggaacgtca tcagatcaaa agaactctcc tcgacgcgca ggctcgttgt
      901 cgaggtatcg ttgatcagtg ccttgatgga gtgcgccatg ggaaacagac cgttcaacgt
      961 cgtcagtatc aggatccctc cggggaccag cgcacgggcc gcactcttca gaatctcgaa
     1021 attttcttca tccgtctcca tcagcggaaa accgccctcg cagagagaaa gggcacaccc
     1081 gaaacaattc tcgaagggca gacgccgggc atctccttct tcaaaactga cagagacatt
     1141 acgcagagcc gccttccccc gggcacgttt gagctgggct ttggacaaat ccaccccggt
     1201 caccggatac ccccgtgccg ccagctcgat cgaatgccgc cctgtcccgc agccaatgtc
     1261 aagaatccgg atgctggtat catgcgccaa aacctcttcg ataaacgcga cctcggactc
     1321 agtatgttgg acaaaatcct ccgtatcgta ctgcttcgcg taatcctcaa acaactcctt
     1381 gtaccacgcc atcatgtcct cccgaatact tttctgccct gatccggccg gccgtcgttc
     1441 cccaaaaagc aatccagcag acggaaggca ccaccccgga taccggcagt atagaccggc
     1501 tgaccgtatc agggaaagcc ccctcgggcg gccatgcagg gattttttct gactcgatgc
     1561 ggaacgggca acaatgccct gcggcggcat gtcaagatca ccgttgggga tatcggtgaa
     1621 aatgattttc gccatgtccg gtgaatgtgg tacaatgtca ggcagcggct gtgattactg
     1681 gcgcaaggaa catccatgga agacgatcca aaactgccga cctcaagctg tgccgtgcgg
     1741 gaattttcca tgcaggcctt gcctcgctgc ccgatcgtct ctacgctgcc cagtggtgtt
     1801 gttgtccatg acgcccaagg ggccatcctc atggccaatc ccgcagccga agagatcctt
     1861 ggcgtcagtc tgtcccagat gcagggaaaa acctccttgg accccatctg gcactgcatc
     1921 cacgaggatg gctccgagta ccccggagag acccacccgg gaatggtcgt cctgacgacc
     1981 cgccagccga tgtacggcgt catcatggga gtttttcatc cccaaaaaac tgactacatc
     2041 tggattgaga tcaatgccct cccgatcctc tcccccgatg ggtatctgaa tggagcctac
     2101 gccaccttta ccgacattac cgctcgtatc cgccacgagg cctccgtccg ggagggctgg
     2161 ctggccatgg atgccatcca cgatctcgtc gcagtgatcg actcggacca tcgctaccgg
     2221 gttgtcaacc aggcctttct cgcacaacgg caggcaacca accaggacat catcgggaaa
     2281 cacgtccgcg aagtccttgg cgaagatgtc tacgaaaggg acat
//
LOCUS       DAOSHE010000358         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45371_length_2322_cov_4.364799, whole genome shotgun sequence.
ACCESSION   DAOSHE010000358 DAOSHE010000000
VERSION     DAOSHE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45371_length_2322_cov_4.364799"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1005)
                     /locus_tag="PLD82_08810"
     CDS             complement(106..1005)
                     /locus_tag="PLD82_08810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG65519.1"
                     /translation="MEFFFAQILLLLTLPFLTLWIILRVKAFWASRRGPALFQPFFDI
                     MRLLKKGAVYSTTTTTVFRLMPTVQLAAVMIAAALLPLPGRDHILSFDGDFVLFAYLL
                     AASRFALVAAALETGSPFEGMGGSREASFAIFAEPAFFLLAGSMVLAGGGTQLAGMVS
                     AFDGRTVLATLAVLSLFVMVLLEGARVPVDDPATHLELTMIHEAMILDHSGPDLAMLH
                     YGAALKTVVVSALLVLVALPEGECTGWMRLLWTAAGTLVLAVLIGTLESFMARLRMTH
                     VPQFVLLMFSISLVALVSVLLLP"
     gene            complement(1005..>2322)
                     /locus_tag="PLD82_08815"
     CDS             complement(1005..>2322)
                     /locus_tag="PLD82_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG65520.1"
                     /translation="EAYPAASPHVSAIMSALMSKLGVYGMLRAVMAFGPSSEVAAWIV
                     VAVGSISAVSGITFAVIRRDLKEILAYSSMENLGIITAGIGVMMLGSAYSNTFMLVAG
                     IAAVLVHTICHSLYKIVLFTGAGLVEYAGAGRDLETLGGLARRMPRIAGTFFCMAWAS
                     ASLPPFGGLISEILLVLGLVAGLTTGFPLVTTALVVILALVIMTGAVALLAWTRIWGI
                     AFSGEPRSAGSAETDDRAVRGWAFLWIPVAVLLFMGLFPAVVFDLLLPFLDTLSLEMM
                     PIDSGIGLWPLVRVSLPETLLSFSVPVIAFVAVIGFLLMVRAMLLRGRRVVAAATWAC
                     GWQKPDPSIQYTADSFSDPFVSVVRPVLHTEYEGEAVRGFFPESAGSNRQDDDPIRRK
                     FVSRLGRLVDGVFARFAWIQSGNMQQYILYGLLWLSAMITWILVVA"
BASE COUNT          621 a          723 c          648 g          330 t
ORIGIN      
        1 ccctgatcac cgcatggatc cggctggtaa acgagatggc gagcatcgtc gccccgatca
       61 tgatgatgac aaactctccg accatacaaa cctccagatc cgccgtcagg gcagaagcag
      121 cacagacacg agtgccacca gggatatcga aaacatcaaa agcacaaatt gcggaacatg
      181 ggtcatgcgc aggcgtgcca tgaaggactc gagagtcccg atcaacaccg ccagaaccag
      241 cgtgccggcc gcagtccaga gaagccgcat ccaacccgta cactcaccct ccgggagggc
      301 gaccagcacc aggagagccg aaacgacaac ggtcttgagc gctgctccat agtgcagcat
      361 ggccagatcg ggcccgctgt ggtccaggat catcgcctca tggatcatgg tcagttcaag
      421 atgggtcgcc ggatcatcaa cgggaacacg cgccccttcc agcaagacca tcacgaacag
      481 ggagagaaca gccagggtag ccaggactgt ccgcccgtca aacgccgaaa ccatcccggc
      541 cagctgtgtc ccgccgccgg ccagaaccat cgaaccggca agcagaaaaa aagccggttc
      601 ggcaaaaatc gcaaacgagg cctcccggct tccgcccatc ccttcaaagg gggaaccggt
      661 ttcgagagcg gcggccacca gtgcaaaccg cgaggccgcc agaagatagg caaagaggac
      721 aaagtctccg tcaaacgaaa ggatgtgatc ccggcccggg agtggaagca gggccgccgc
      781 aatcatgacg gccgccaact gcacggtggg catcagccgg aaaaccgtag tcgtcgttgt
      841 cgaatagacc gcgccttttt tgaggagtcg catgatgtca aaaaacggct ggaacagggc
      901 cggccccctc cgggatgccc aaaaagcctt aaccctgagg ataatccaga gtgtcagaaa
      961 aggaagagtg aggaggagaa gaatctgggc gaaaaagaat tccatcacgc caccaccaga
     1021 atccaggtaa tcatggccga cagccagagc aacccgtaga gtatgtattg ttgcatattg
     1081 ccgctttgaa tccaggcaaa acgggcaaaa accccatcga caagacgtcc cagccgggaa
     1141 acgaatttcc gcctgatggg atcgtcatcc tgccggttgg atccggcact ctccggaaaa
     1201 aatccccgca cggcctcgcc ctcgtactcg gtgtgcagca caggacggac aacgctcaca
     1261 aacggatcgg aaaacgagtc cgccgtgtat tgaatcgaag gatccggttt ctgccaacca
     1321 caggcccagg tcgcagcagc gacaacccgc ctccccctga gaagcatggc ccggaccata
     1381 agcagaaatc cgatgactgc gacgaaagcg atcaccggga cactgaagga gagcaaggtc
     1441 tccggcagac tgacccgcac cagtggccag agcccgatcc ccgaatcgat tggcatcatc
     1501 tcgagcgaga gcgtatcaag gaatggcagc agcaggtcga aaacgaccgc cgggaagagc
     1561 cccataaaga gcaggaccgc aaccggaatc cagagaaatg cccacccccg gacagcacgg
     1621 tcatccgttt ccgccgatcc ggcactccgg ggctcacccg aaaacgcgat gccccagatt
     1681 ctggtccatg ccaggagcgc caccgcacca gtcatgatca ccagtgccag aatgaccacc
     1741 agagcggttg ttacgagagg aaagcccgtt gtgagccctg cgaccagacc caaaacaagg
     1801 aggatttcgc tgatcagccc accaaagggc ggaagactgg cactggccca agccatacag
     1861 aagaatgttc cggcgatgcg cggcatccgg cgggccagtc cgcccagcgt ctcaagatcc
     1921 cgtccggcac cggcatattc cacgaggccg gccccggtaa agaggacgat tttatagagt
     1981 gagtgacaga tggtatggac gaggaccgca gcaatcccgg ccaccagcat gaaggtattg
     2041 gagtaggcac tcccaagcat catcacaccg attccggccg tgatgatccc cagattttcc
     2101 atactgctgt atgcaaggat ctccttgagg tcccgccgga tcacggcaaa ggtgataccg
     2161 ctaacagcgg agatactgcc gacggcaaca acgatccagg cagcgacttc tgaagacggc
     2221 ccgaaggcca tcaccgcacg caacatcccg tacaccccga gtttgctcat caatgcggac
     2281 atgatggccg acacatgggg ggatgccgcc gggtaggcct cc
//
LOCUS       DAOSHE010000359         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45380_length_2322_cov_3.188796, whole genome shotgun sequence.
ACCESSION   DAOSHE010000359 DAOSHE010000000
VERSION     DAOSHE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45380_length_2322_cov_3.188796"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLD82_08820"
     CDS             complement(<1..791)
                     /locus_tag="PLD82_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG65521.1"
                     /translation="MVRQMVMLAAVLLCLLSCGQEAGPPVPETGTGKRPVIGLVLSPN
                     GLGDMSYNDMQYNGLIEAYRRHDIEVRFRVPQIDTPAAITALCTDLIRTEGCTLLMAG
                     EGVRMTPIFEKLALQHPRVMFALLGYQGKPIPNLVSSRFAQYEGSFVTGVLAARMAGQ
                     GGIAFIGGVNIPAIAEFSGGFVDGVRHARPGTAVTVRHISLFPDFSGFDNPQAGYSLA
                     RELYASGVNVIYGAAGASGNGIIQAAKESGKYVIGVDSNQDHMAPG"
     gene            946..1836
                     /locus_tag="PLD82_08825"
     CDS             946..1836
                     /locus_tag="PLD82_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG65522.1"
                     /translation="MRNGYRIVDGHAHIFPDGDISAKIMTSFNERFLIPFPPAGSGTV
                     DALLDDMRNAGIDFTILANFASDRFLHENNLWTLSLARFSPNLVPLVSFSPNLLAEHS
                     VDSLFTAYLAQGAKGIKLHPMAQSFDPGDPAILPVYSLAGQHGIPVVFHCGRVANARL
                     NGWSDYEVLLPLIEAFPETNIVLTHMVDGSEEDLRNAARYPNVFFDTSIVISGYSHLL
                     DSNEPSWKEDGRFVTLVREIGATRFLFGSDHPWGSARADLARFFRMPLEPDETRAILG
                     ENATRLFAIGKKNGESHEQP"
     gene            1823..2038
                     /locus_tag="PLD82_08830"
     CDS             1823..2038
                     /locus_tag="PLD82_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOG65523.1"
                     /translation="MSSPEREENAEERIIRLEQRIAWLEETLASLDSAVTRQEREIRS
                     LSRQLSTLRESFENPQARNLTDRPPHW"
     gene            complement(2052..>2322)
                     /locus_tag="PLD82_08835"
     CDS             complement(2052..>2322)
                     /locus_tag="PLD82_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65524.1"
                     /translation="TRHAWMLSSEYRMAVYRDIIKFIFFVDTALYLPLQYDHGVHNLK
                     PRIRTSVGQGVEFNFHEWSLKLYYGVPSYKEVFEGKLHLSIQKVF"
BASE COUNT          510 a          707 c          626 g          479 t
ORIGIN      
        1 ccgggagcca tgtgatcctg gttggagtca actccgatca cgtatttccc gctttccttc
       61 gcggcctgga tgatcccgtt accgctggca ccggccgccc cgtagatcac attgacgccg
      121 gaggcataga gttcacgagc cagactatac ccggcctgtg gattgtcaaa cccggaaaaa
      181 tccggaaaaa gactgatgtg gcggacggtg acggcggtac ccggacgggc atgccggaca
      241 ccgtcgacaa aaccgccgga gaactcggct atcgccggga tgttcacacc cccgataaag
      301 gcaatcccgc cctgcccggc catcctggca gcaaggaccc cggtcacaaa agaaccctca
      361 tactgggcga agcggctcga gacaagattt ggtatcggct ttccctgata tcccaataac
      421 gcaaacatga cccggggatg ctggagggcg agcttttcga aaatcggggt catgcgcacc
      481 ccttcgccgg ccatgagcaa ggtacagccc tcggtccgga tcaaatcagt gcagagcgcg
      541 gtgatcgccg cgggagtatc gatctgtgga acccggaacc ggacctcgat atcatgccgc
      601 cggtaggcct cgatcagacc attgtactgc atgtcgttgt aggacatgtc ccccagaccg
      661 ttgggcgaga ggaccagtcc gatcacggga cgttttccgg tacccgtctc gggaacaggc
      721 ggcccggcct cttgtccaca ggagaggagg caaagcaaca ctgcggccag catgaccatc
      781 tgtcgtacca tcgtccccac cctggtgcac cacaaagccg tatcgagtca tggtatcacg
      841 tcactgacat cataatacac attttccgtg atggttctca atgctttttt tggtgaacat
      901 cctcaggccg acccgaaggg cagatgaata tgctatattt ttttgatgag aaacggctat
      961 cgtatcgttg acgggcatgc ccacatcttc cccgacggag acatctccgc aaaaatcatg
     1021 acgagtttca atgagcgttt tttgattccg ttcccgcccg cgggcagtgg aacagtcgat
     1081 gcgctcctgg atgacatgcg gaatgccggc atcgatttca cgatcctggc gaattttgcc
     1141 agcgaccggt ttttgcacga gaacaatctc tggacgcttt cgctcgcccg tttctcgccg
     1201 aacctcgttc cccttgtcag cttttccccg aacctcctcg ccgagcacag cgttgactcg
     1261 ctctttaccg cctacctggc ccagggtgca aaagggatca agctccatcc catggcccag
     1321 agctttgatc cgggtgaccc ggccatcctt cccgtctaca gcctggccgg tcagcacggg
     1381 atcccggtcg tctttcactg tggacgtgtg gccaacgccc gactgaatgg ctggtcggat
     1441 tacgaagttc tccttcctct gatcgaggcc ttccccgaaa cgaatatcgt tttgacccat
     1501 atggtcgatg gctccgagga ggacctccgg aatgccgcac ggtatccgaa cgtctttttt
     1561 gacacatcaa tcgtgatctc cggatacagt catcttctcg attccaatga accatcatgg
     1621 aaagaggatg gacggtttgt caccctcgtc cgcgagattg gagcaacccg cttcctcttc
     1681 gggtccgatc acccctgggg atcagcacgg gcggatctcg cccggttttt ccgcatgccc
     1741 cttgaacccg acgagacacg ggccatcctc ggggagaatg cgacccggct ctttgcgatc
     1801 ggcaaaaaaa atggagagtc acatgagcag ccctgaaaga gaggagaacg ccgaggagcg
     1861 gatcatccgt cttgaacaac ggatcgcctg gctcgaagag accctggcca gcctggactc
     1921 ggccgtcacc cgtcaggaac gcgagatccg gtcgctctcg cgccaactct caaccctgcg
     1981 cgagagtttc gagaatccac aggcccgcaa cctgacggat cgcccgcccc actggtgagc
     2041 cgagagccgc ctcaaaagac tttctggatc gagagatgga gctttccttc aaagacttcc
     2101 ttgtaggacg gcaccccgta atagagcttg agtgaccact catggaagtt gaactccacc
     2161 ccctggccga ccgaggtacg gattcggggt ttgagattgt gcacaccatg atcatactgc
     2221 agcgggagat agagggcagt atccacaaaa aagatgaact tgatgatgtc gcgatagaca
     2281 gccatccggt actcgctgct caacatccat gcatgccggg tg
//
LOCUS       DAOSHE010000360         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45621_length_2313_cov_3.041630, whole genome shotgun sequence.
ACCESSION   DAOSHE010000360 DAOSHE010000000
VERSION     DAOSHE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45621_length_2313_cov_3.041630"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLD82_08840"
     CDS             complement(<1..1513)
                     /locus_tag="PLD82_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOG65525.1"
                     /translation="MDFFETSIRCLEAAAPVLARRVREARDTTRYRIEDAKTGDATLL
                     FVDGTGHERLMHSRHNPRREARRIVETSIDPDSDSVIVGGFGLAWHIDAIMEHAPNAA
                     VIVVEDEPAVLAAACMARDLTPLFTSGRIDLALGERTDDVLTLLEGKPSRKVAVFLHR
                     ASADRNPGFYHNLKNILYSWLNGKEVNLATLVRFERLWTRNLLRNLPFFAKAAGVASL
                     FGTTKDLPVFVVAAGPSLAKNIHLLQRAGSRGIIIAVDTIYKVLLAHGITPDIVVAVD
                     PQLVNAQFFLGTPSSRTILVSDSAVSPTLLANHKGPLAISAVPFPFASWFESFFGHKG
                     ALSSGGSVATSAFDLAVQMGGNPVVLLGQDLAYSSERIHMRGTSGEERWENTCTRTTP
                     VTRNMRSFLRQNATVRVRSWDGQSEVWSDRKFLTFLWWFERQIRMIDGRIRIVNATEG
                     GAHIKGAEHISLESLLETLPERQVTHDLPTSDASSAGITACLAAARDFSDFLRS"
     gene            complement(1533..>2313)
                     /locus_tag="PLD82_08845"
     CDS             complement(1533..>2313)
                     /locus_tag="PLD82_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG65526.1"
                     /translation="GNLGFGLPTGISLPGEASGILRPVQKWSGLSRYMLSIGYECSVT
                     PLQLVRAASALATGGVLMQPVLVKAVTDHDGHILEASAPAVVRRVLKPSTTTRIMAML
                     RNVVVAGTGIQANLGELEAGGKTGTAYVAKAGGGGYYKTRYTASFMGFLPWKNSNLAI
                     LVMIYEPRGQYEGGTLAAPVFRRIARRTLLYLNQKLPPVIDEQSMQRVILPGLTESQR
                     NNLLRAYKRDSTRKRFILSGELTDHQKTELSLILQSAGQLQ"
BASE COUNT          453 a          665 c          733 g          462 t
ORIGIN      
        1 ggctccggag aaagtccgaa aagtcacgcg cggctgccag gcaggccgtg attcctgctg
       61 acgaggcgtc gcttgtgggc aggtcgtggg tgacctgtct ttcggggagg gtctcgagca
      121 gggattcgag gctgatgtgc tccgcgccct tgatgtgggc cccgccctcg gtggcattca
      181 cgatccggat ccggccgtcg atcatccgga tctggcgttc gaaccaccag agaaacgtca
      241 aaaacttgcg gtcactccag acttcgcttt gtccgtccca ggatcgcaca cggaccgttg
      301 cattctggcg cagaaaactg cgcatattcc gggtgaccgg ggtggttcgg gtacaggtgt
      361 tttcccagcg ttcctcgcca ctcgtgcccc gcatatggat tcgttcggaa ctgtaggcca
      421 ggtcctgccc caggagaacg acgggatttc cacccatctg gaccgcaaga tcaaaggcac
      481 ttgtggcgac cgaccctccc gacgagagcg cccccttgtg gccaaaaaag ctctcaaacc
      541 atgaggcaaa gggaaatggc accgccgaga ttgccaaggg gcccttgtgg ttggcgagga
      601 gggtgggtga aaccgcagag tccgagacga gtattgtccg gctggacggc gttcccagaa
      661 aaaattgtgc attgaccagt tggggatcaa cggcgacaac gatatccggc gtgatcccgt
      721 gcgcgaggag gaccttgtag atcgtgtcca cagcgatgat gatccctcgg gagcctgccc
      781 gctggagaag gtggatgttt ttcgccagtg atggcccggc ggcaaccaca aagaccggaa
      841 gatccttggt ggtcccgaag agggaagcca cacccgctgc tttggcgaag aagggaagat
      901 tgcgcaggag gttccgggtc cagaggcgtt caaaccggac caaggttgcc agattgactt
      961 ctttgccgtt caaccaggag tagagaatgt ttttgagatt gtggtagaat cccggattgc
     1021 ggtccgcgct ggcgcggtgc aggaagacgg caaccttgcg cgagggtttt ccctcaagca
     1081 gggtcagaac atcgtccgtc cgttctccga gagccagatc gatccggccg ctggtgaaga
     1141 gcggggtcaa atcccgcgcc atgcaggccg cggccaacac ggccggttcg tcctcgacca
     1201 cgatcacagc cgcgttcggg gcatgttcca taatggcatc aatgtgccat gccagcccga
     1261 acccgcccac gatgacggag tctgaatccg gatcgatgct ggtttcaaca atccggcggg
     1321 cttccctgcg gggattatgc cggctgtgca tcaggcgttc gtgtccggtt ccgtcgacga
     1381 acaggagggt ggcatcaccg gtcttggcgt cttcgatccg gtagcgggtt gtgtcccttg
     1441 cctcgcggac acgccgggcg aggaccggtg cggcagcctc gagacagcgg atacttgttt
     1501 caaaaaaatc cataccgccc ggtctccgct gtttactgaa gctggccggc actctgcagg
     1561 atgagcgaga gttccgtttt ttgatgatcg gtcagttcac cggagaggat gaagcgtttt
     1621 cgtgtggagt ccctcttata ggccctgaga agattgttcc gctgggactc cgtcagtccg
     1681 ggaagaatga cacgctgcat gctctgttca tcgatgacag gcgggagctt ctggttgagg
     1741 tagaggagtg tccgccgggc gatccgccgg aagaccggag ccgccagggt cccaccctcg
     1801 tactgtcccc ggggttcgta gatcatcacg aggatcgcca gattgctgtt tttccacggc
     1861 aggaaaccca taaaggatgc ggtgtagcga gttttgtaat acccgccgcc accggccttt
     1921 gccacgtagg cggtcccggt ttttcctccg gcttcaagtt ccccgagatt ggcctggatt
     1981 ccggttcccg ccacgacgac attgcgcagc atggccatga tccgggtggt ggtggacggt
     2041 ttcaaaacac ggcggacaac ggctggcgcc gatgcctcga ggatatggcc gtcgtgatcg
     2101 gtgaccgcct tgaccaacac cggttgcatc aagacaccac cggtcgcgag cgcagaggct
     2161 gccctgacca gctgcagggg ggtaaccgaa cactcatacc cgatcgaaag catgtaccgc
     2221 gacagcccgg accatttttg gaccgggcgc aggatcccgg aggcttctcc tggcaggctg
     2281 atcccggtcg ggagtccgaa gccaagattt ccc
//
LOCUS       DAOSHE010000361         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45635_length_2313_cov_2.327724, whole genome shotgun sequence.
ACCESSION   DAOSHE010000361 DAOSHE010000000
VERSION     DAOSHE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45635_length_2313_cov_2.327724"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLD82_08850"
     CDS             complement(<1..235)
                     /locus_tag="PLD82_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65527.1"
                     /translation="MRFFSFLLVGFMAVGCNVIEETTIVHAAGSSLPTTAASSLSSQS
                     SMNSLASLSSYSSFSSSSSAGSAVPLAWVTVSNG"
     gene            466..1209
                     /locus_tag="PLD82_08855"
     CDS             466..1209
                     /locus_tag="PLD82_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65528.1"
                     /translation="MRIMCAILSLILTGLTCPFSAGAVSPVRISGFLCLDPDLQIPPE
                     GRRVTISLEDRKWRPVARTALAVHPGDTRIPWSLSVSQPDLAPGRLYLGYRISEDTGS
                     HGRGWWSPDGTVYDQRSAGQILLSNGNANQIHLCILRGTGLQGRLYLDPDNRPRSVGI
                     RLIDQSGRSRGTRWYPVHPDSTRIDWARTWPPQSTPERVRIQIILHAQHSKRTFWGRG
                     KGLTVDPHKADEYSLSTRNRLVLEDALVD"
     gene            1349..2254
                     /locus_tag="PLD82_08860"
     CDS             1349..2254
                     /locus_tag="PLD82_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HOG65529.1"
                     /translation="MTVPYGKGWLSVDDQIELLALRGLTIDDHSGAREFFSHVNFNRF
                     DNYCRSFESKRHVFLPGTSFSDVMALYDFDGSLRELLSRAIRVFEIDLRTIIASAWGS
                     RHGAFGHRNRNNFFRSFAHADWLTGLQGETIRSREFFVKHYRETYQEYPDLPVWMAVE
                     IMPMRWLSAMFSGMKRPDQEPILARYEVSPVILASWLHHLTYVFNLTTHHARIWDRLW
                     SISPKLPRAFDWMPPSLPAGNRLFSTLLILSHLLSRCPRQRAMTALWRASIESHIDTI
                     PPVPDALFRMGLPEDWKNHPEWIAP"
BASE COUNT          479 a          759 c          603 g          472 t
ORIGIN      
        1 aaccgttgga aacagtcacc caagccagcg gtacggccga acctgccgaa gacgacgagg
       61 agaaagaaga ataagaagag agggaggcga gagaattcat gctcgactga ctcgaaagtg
      121 acgaggccgc cgttgtcggg agcgaagaac ccgctgcatg aacaatcgtc gtttcttcga
      181 tgacattgca tccgaccgcc atgaaaccaa ccaagagaaa agagaaaaac cgcacgtgat
      241 ccctccatac ccgtcttccg attcttgcca caaaatcgcc gacatattat acagtatccg
      301 tccccctgcc tgtcaagtca tccatgcaca tcctgaaccg gagagtctgc aattgatcaa
      361 ttccgatgat gcgtttcccc ggtctccagt attttcttgc ctgattgcca gcccgatcgg
      421 gccgggctac acttgaagcg agaccaccag ccggagtgta cgcgcatgcg gatcatgtgt
      481 gcgatcctga gcctgatcct gacaggtctg acctgtccct tctctgccgg tgcggtttcc
      541 ccggtgcgga tcagcgggtt tctctgtctc gacccggatc tgcagattcc gcccgagggc
      601 cgacgcgtca caatatccct tgaagacagg aagtggcgtc ctgtcgcccg cactgctctc
      661 gctgtccatc ccggagatac caggattccc tggtctcttt ccgtcagtca gcccgatctg
      721 gctccgggcc gcctgtatct cggctacaga atcagcgaag atacaggaag tcacgggcgg
      781 ggctggtggt cacccgatgg caccgtgtat gaccagcgat cagcgggaca gattctgctc
      841 tccaacggaa acgcaaacca gatccacctc tgcatcctgc gcggaacagg gctgcagggc
      901 agactgtatc tcgatccgga caacaggcca cgctccgtcg gcatccgctt gatcgaccaa
      961 agcgggcgca gcaggggaac acgctggtac ccggttcacc ccgactccac ccgaatcgac
     1021 tgggcacgga cctggccccc gcagagcaca ccagaacggg tccggattca gatcatcctc
     1081 cacgcccaac actcgaagcg gaccttctgg ggacggggca agggcttgac agtggatcca
     1141 cacaaagccg atgaatacag cctctccacc cgcaaccggc tcgtcctgga agatgccctt
     1201 gtcgactgaa aggggcggag agtgaaaaaa attcttgcac tcgcctccag gaggaggcat
     1261 actctcccca gcacacgcgc cgggcgttcc tttctctcgg gacaggacgc acaagagagg
     1321 cgcgtatttt tttgtccgga gtcctggcat gactgtccca tacggcaaag gctggctctc
     1381 ggtcgatgac cagatcgagc tgctcgcctt gcgcggcctc accatcgacg accattcggg
     1441 agcccgggaa tttttcagcc atgtcaattt caaccgtttt gacaattatt gccgctcctt
     1501 cgaatcaaaa cgtcacgtct ttctcccggg aaccagtttt tccgatgtca tggccctcta
     1561 tgattttgac ggttccctgc gcgagctgct ttcccgtgcc atccgggtct tcgagatcga
     1621 tctgcgcacg atcatcgctt cggcctgggg ctcgcgccat ggagccttcg ggcaccgcaa
     1681 ccgcaacaac tttttcaggt cgtttgccca cgccgactgg ctgaccggtc tccaggggga
     1741 aaccatccgt tcgcgcgagt tctttgtcaa acactaccgc gaaacctatc aggagtatcc
     1801 agatcttccc gtctggatgg ccgtcgagat catgcccatg cgctggctct cggccatgtt
     1861 ttccgggatg aaacgcccgg accaggagcc aatccttgcg cggtacgaag tatcacccgt
     1921 gatcctcgca agctggctgc accacctgac ctacgtcttc aacctgacaa cgcaccatgc
     1981 ccggatctgg gaccggctct ggtcgatcag tcccaagctc ccccgcgcct ttgactggat
     2041 gccgccctcc ctccccgccg gaaaccggtt gttctcgacc ctcctcatcc tctcgcatct
     2101 tttgtcgcgt tgcccccgcc aacgggccat gaccgcactc tggcgggcat ccatcgaatc
     2161 ccatatcgat acaatccccc cggtcccgga tgcgttgttc cggatgggcc tccccgaaga
     2221 ctggaaaaac catcccgaat ggattgcccc atgaaacgat tcgcacgcgt tgcttcctgc
     2281 tgcctgattc tcctctcctg cacgggcgga ccg
//
LOCUS       DAOSHE010000362         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_45862_length_2305_cov_3.274222, whole genome shotgun sequence.
ACCESSION   DAOSHE010000362 DAOSHE010000000
VERSION     DAOSHE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45862_length_2305_cov_3.274222"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLD82_08865"
     CDS             complement(<1..169)
                     /locus_tag="PLD82_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOG65530.1"
                     /translation="MRDWSLYLVTDRAMLAGREYIQTVLQAVSGGVTAVQLREKELPT
                     RELVTLAKALVQ"
     gene            complement(355..1188)
                     /gene="thiM"
                     /locus_tag="PLD82_08870"
     CDS             complement(355..1188)
                     /gene="thiM"
                     /locus_tag="PLD82_08870"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOG65531.1"
                     /translation="MQSSTQTTAGTILAQLRSEPPLVQIITNYVTINDVANALLCLGG
                     SPAMVEAEAEAASFARIVSALYLNVGTLTAEQARAMPLALAVARDRGIPVVLDPVACG
                     AFPEKMAFVQSLLAQGGVTIIKGNSAEILSLAGMEARSRGVDALESAANPGDACRIAA
                     EKFGAVVMATGPEDSLSDGTQVWRMTGGHPLLGRVSGTGCVLGGLVAAAAAAARRAGA
                     DDAAGCLMAAAVFALAAEQAAALPTTAGPMSFRTALFDRLSTVPQSELDAYAKIAWHM
                     P"
     gene            complement(1188..1982)
                     /gene="thiD"
                     /locus_tag="PLD82_08875"
     CDS             complement(1188..1982)
                     /gene="thiD"
                     /locus_tag="PLD82_08875"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOG65532.1"
                     /translation="MVHALTIAGSDSGGGAGIQADSKTWSALGVYAMTAITAVTAQNT
                     VGVQAIHKIPLDCIRTQIESVFSDIRVDAVKIGMLGDAEIIQTVAEALTRWKPSIIVL
                     DPVMVAKSGDHLLEPSAVRAMTELLIPQASVITPNLPEAEVLTDMKIDSLRDMKEAAR
                     KILALGTNAVVVKGGHLEGQAIDVLSTSDLCLELPAPRLDARHTHGTGCTLSAAITAY
                     LAKGMPLADAVRSAKTYTANAIQNGIPLGHGIGPTHHFHAIWKETV"
     regulatory      complement(2058..2157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          469 a          642 c          740 g          454 t
ORIGIN      
        1 cctggacgag ggctttggcc agcgtcacca gctcacgggt cgggagctcc ttttccctga
       61 gttgtacggc tgtcacacca ccggatacag cctgcaacac cgtctggata tactctcgtc
      121 cggccagcat ggcccggtcg gtgacgagat agagactcca gtctctcatg attatcctcc
      181 tgcaaacggt ggtcttggcc ggttgctgta cagctttcag gcttggcgac atgatcccgc
      241 ctctttcttc atcatacatg cgattgaccg gcaggacaga aaaaagtgct gtcgaagatg
      301 aaaccgcatg actggaaacg tcgggaaaaa aagcgtgcgg gcagagaggg gtgttcaggg
      361 catgtgccag gcgattttcg catacgcatc cagttctgat tgaggaacag ttgacagtcg
      421 atcaaaaagc gcggttcgaa aagacatcgg tccggccgtc gtcgggaggg cggccgcctg
      481 ttcagccgcc agggcaaaca cagcggcggc catcagacag ccagccgcat catccgcacc
      541 ggccctgcgg gctgcagctg cggcagccgc aacgagcccc ccgagcacgc agcccgtgcc
      601 actgacgcgg cccaggaggg ggtggccgcc ggtcatgcgc cagacctgtg tcccgtcgga
      661 gagagagtcc tcgggccccg tcgccatgac aacggccccg aatttttcgg cagcaatccg
      721 gcatgcatct ccgggattgg cagcgctctc aagtgcatcg actccgcggg atcgtgcttc
      781 catgccggcc aggctcagaa tctcggccga gtttcccttg atgatggtca caccaccctg
      841 ggccaggaga ctctggacga aggccatttt ttcagggaaa gcgccacagg cgacaggatc
      901 aagcacgacg ggaattcctc gatcccgggc cacagccaga gcaagaggca tggcgcgtgc
      961 ctgttcggcg gtcagcgtac cgacgttgag gtagagagcg gaaacgattc tggcaaaact
     1021 ggctgcctcg gcttcagctt caaccatggc gggcgaaccg cccaggcaga gcagggcgtt
     1081 ggcaacatcg ttgatggtga catagtttgt gatgatttgc accaggggcg gttcgctcct
     1141 gagttgtgca aggatggttc ctgctgttgt ctgcgttgac gattgcatca gactgtctcc
     1201 ttccagatgg catgaaaatg atgtgttggc ccgattccgt gacccagggg aattccgttc
     1261 tggatggcgt tggccgtata cgtcttggcg gaacggaccg cgtcggcgag gggcatcccc
     1321 ttggcgagat acgcggtgat ggcggcggag agggtgcatc cggtcccgtg ggtgtggcgg
     1381 gcatcaagcc ggggggccgg gagttcgaga caaagatcgg aggtggacag gacatcaatg
     1441 gcctgaccct ccaggtggcc gcccttgact acgacggcat ttgttcccaa tgcgagaatt
     1501 tttcgggcag cttccttcat gtcgcgcagt gagtcaattt tcatgtcggt caggacttca
     1561 gcttcgggca gattcggggt aatgacactg gcctgaggga tgagaagctc agtcatcgcc
     1621 cgaaccgcgg agggttcaag aaggtgatca ccgctttttg caaccatgac agggtcgaga
     1681 acaatgatgg acggtttcca gcgggtgagg gcctctgcaa cagtctggat aatctcggca
     1741 tcgccaagca tcccgatctt gacggcatcc acccgaatgt ccgaaaaaac actctcgatc
     1801 tgggttctga tgcagtcgag gggaatcttg tggatggcct gaaccccgac ggtattttgg
     1861 gcggtgacgg ctgtgatcgc cgtcatggcg tacacaccga gagcgctcca ggtcttgcta
     1921 tctgcctgga tcccggcgcc accgccagaa tccgaaccgg caatcgtaag agcgtgaacc
     1981 atgatggaag cttcctttcg ttctcttgag gcatgggctg caggggcacc tgggcgaaag
     2041 gaaggcgggg caaaaaaagt atgcgttgcc tgcttcccta cgccggtatt atccgaacag
     2101 gttcaagggg tcaggcatac gccttctcag ccggaatcgg cacccccagc agcttgtgat
     2161 gaaagtctag tatcggggat gcgttctggc aagcaatact cccggccgcc ggacaaaggt
     2221 tactcttgcc gagcacttcg cccgcaggga gagggtgccg ggagggtgcc gggagggtgc
     2281 cgggagggtg ccgggagggt gccgg
//
LOCUS       DAOSHE010000363         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46028_length_2299_cov_3.305258, whole genome shotgun sequence.
ACCESSION   DAOSHE010000363 DAOSHE010000000
VERSION     DAOSHE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46028_length_2299_cov_3.305258"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="tsaB"
                     /locus_tag="PLD82_08880"
     CDS             <1..616
                     /gene="tsaB"
                     /locus_tag="PLD82_08880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG65533.1"
                     /translation="LALLKDGEFISMQSDGVEPSASRMYVLMDAVLRAGSTGIGDVTA
                     LALGAGPGSFTGLRVSFAALRTLAWARGIPLIPVNSLEALVWAHRALPGPIAVVQPAR
                     RGHVYQLAVRQGRIVQEVGFFSLEEARARIGELGEGVLLAGIPDDLELGLPVLVQRAG
                     PDAAAIARIASARLAAGERPILEDVLPLYLRPSDAEVTAGLGVG"
     gene            710..>2299
                     /gene="ptsP"
                     /locus_tag="PLD82_08885"
     CDS             710..>2299
                     /gene="ptsP"
                     /locus_tag="PLD82_08885"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG65534.1"
                     /translation="MTRIRHNERIIEIEDAIVAAPGIAIGVAVILSDNILYTPEYTIM
                     RSQIEDELNRLNAAIERARQELIARQEELSEHFGKAVDLLDPMLKMLSDPALHRDIRG
                     LIEKRLKNIEVIFAEVIDSYIEKLASTNDRYFRERADDLRHIRTHVMLTLLGRTETNL
                     RNLSEDTIVVASTLSPSQTAAMDKKKVKGIVTELGGKTSHSAILARALEIPTIVSASD
                     ITKEIRAGDVLIVDANHCKIIVNPGEQVIKEYQTARSVYDKFINKLRYIDDLPAETKD
                     KARITVSANIGMLEELDAAMTHGADGVGLFRSEFLFVERNDFAGEDEQYQIYRKVLEK
                     MGKRPVVIRTIDFGSDKPSHEHRKYPEANPSLGWRSIRFCHSMPNVFKTQLRALYRAS
                     VHGNLSIMVPMISSVEELLKTRHFLEQARKELAKEAVPFNEDMKFGIMIELPSAVLMA
                     DELARHCDFFSIGTNDLAQYTLGVDRGNKKVAHLYKVSHPAVLKLIRMTIEAAEKRGI
                     PVSMCGEMASNLHYTQLLMGMG"
BASE COUNT          488 a          655 c          669 g          487 t
ORIGIN      
        1 cctggccctg ctgaaggacg gtgagttcat ctccatgcag tcggatggcg ttgagccgtc
       61 cgcatcgagg atgtatgttc tgatggatgc cgttctccgg gcgggctcga cgggtattgg
      121 agatgtgacg gcgttggctc ttggtgccgg cccgggttct tttaccggcc tgcgagtttc
      181 gttcgccgct ctgcgcacac tggcctgggc ccgcgggata ccgctgattc ccgtcaacag
      241 tctcgaagcc ctggtctggg cccaccgtgc cctgcccggg ccgatcgccg tggtgcaacc
      301 ggcccggcgc gggcatgtct atcaactcgc ggtccgtcaa gggcggattg tccaggaggt
      361 cgggtttttt tcccttgaag aggcccgggc gcggatcggc gaactggggg agggcgtcct
      421 gcttgccggg atcccggatg accttgagtt gggtcttccc gttctcgtgc agcgcgccgg
      481 accggatgcc gccgcgatcg cgcggatcgc ctcggcccga ctcgcagccg gagagcggcc
      541 gattcttgaa gatgtcctgc cgctctatct ccgtcccagc gatgccgaag tgacagcagg
      601 actcggtgtc gggtgattca ggcttgtttc atgcggcgtt caacccggat gtattccgta
      661 ttgaagaaaa cgcttgattt tttacacgta tgcgtcatac tctcaaacga tgacacgaat
      721 caggcataac gagcgtataa tcgagattga agacgcaata gttgccgcgc caggaattgc
      781 catcggtgtg gctgtgatcc tgtctgacaa tattctctat actcctgaat acacgatcat
      841 gcgcagccag atcgaggacg agctcaatcg cctcaatgcg gccatcgagc gcgcccggca
      901 ggaattgatc gcccgtcagg aggaattgtc cgagcacttt ggcaaagcgg ttgatctgct
      961 tgatcccatg ctcaagatgc tctccgatcc ggccctgcac cgggatattc gtggtctgat
     1021 cgaaaaacgg ctcaaaaata tcgaagtgat ttttgccgag gtcatcgaca gctatatcga
     1081 aaaactggcc tcgaccaatg accgctactt ccgtgaacgg gcagatgacc tcaggcacat
     1141 ccggacacat gtcatgctga cgcttcttgg acggaccgag accaatttgc ggaatctctc
     1201 cgaggatacc atagtcgttg ccagtacgct ctctccgtcc cagacggcgg ccatggacaa
     1261 aaaaaaggtc aagggaatcg tgaccgaact gggtggaaag acatcgcatt ccgcgattct
     1321 ggcccgtgct ctcgagatcc cgaccatcgt gagcgccagt gacatcacaa aggagatccg
     1381 ggccggtgat gtgctgatcg tggacgccaa ccactgcaag atcatcgtca atccgggcga
     1441 gcaggtcatc aaagagtatc agactgcgcg cagtgtgtat gacaaattca tcaacaagct
     1501 gcgctatatc gatgacctgc ctgccgaaac caaggacaag gcgcggatca cggtttcggc
     1561 caatatcggg atgctcgagg aactggatgc cgcgatgacg catggtgcgg acggagtcgg
     1621 actcttccgc tccgaattcc tctttgtcga gcgcaacgat tttgccggtg aggacgagca
     1681 gtaccagatt taccgcaagg tcctcgaaaa aatgggaaag cggcccgtgg tcatccggac
     1741 catcgacttc ggaagcgaca aaccatcgca tgagcaccgc aagtatcccg aggccaatcc
     1801 atccctcggg tggcgttcca tccggttctg tcacagcatg cccaatgtct tcaagacgca
     1861 gttgcgggca ctctaccggg catcggtcca cggaaacctc tcgatcatgg tccccatgat
     1921 ttcgagtgtc gaggagttgc tcaagacccg gcattttctc gaacaggccc gcaaggagct
     1981 tgccaaggaa gccgtcccct tcaacgagga catgaagttc gggatcatga tcgagcttcc
     2041 ctcggcggtg ttgatggctg acgaactggc ccggcattgt gatttcttca gcattggaac
     2101 aaacgatctg gcccagtata ccctcggtgt tgatcgtgga aacaaaaaag tcgcacacct
     2161 ctacaaggtc tcgcacccgg ctgtgctcaa gctgatccgg atgacaatag aggccgccga
     2221 gaagcgaggg attccggtct cgatgtgcgg cgagatggcc agcaatcttc actataccca
     2281 gctgctcatg gggatgggc
//
LOCUS       DAOSHE010000364         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46236_length_2292_cov_4.862316, whole genome shotgun sequence.
ACCESSION   DAOSHE010000364 DAOSHE010000000
VERSION     DAOSHE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46236_length_2292_cov_4.862316"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLD82_08890"
     CDS             complement(<1..329)
                     /locus_tag="PLD82_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOG65535.1"
                     /translation="MPPVRANLTPADLLIVLVPLALAVFLFSRETGDGPSAVRITTPQ
                     GVWRYTLPAAKELRFEGSAGAFLVEIKGGQVSIRETHCPDRLCQEMGPLEKSGAVMIC
                     VPQKIEVR"
     gene            complement(329..832)
                     /locus_tag="PLD82_08895"
     CDS             complement(329..832)
                     /locus_tag="PLD82_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65536.1"
                     /translation="MKSLHLMIILAVWAGCTAHRDGLRQGTTDTWSAESRGFELTIPS
                     TARFTGSSVQPYTLSIHDGKTGIRAALEILDAGKDCTAILEERIGAIKGASILGRGQM
                     MMWGRAGVIARVTAPAGRILLTVINLRQQICVLTLEQAANGLDDDAALDAAQGFLDAL
                     LNGMRFS"
     gene            complement(829..2196)
                     /gene="hslU"
                     /locus_tag="PLD82_08900"
     CDS             complement(829..2196)
                     /gene="hslU"
                     /locus_tag="PLD82_08900"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOG65537.1"
                     /translation="MNSNPLITGMTPQEIVSELDKYIMGQDDAKRAVAVAVRNRSRRM
                     AVDPAMRDEILPKNILMVGPTGVGKTEIARRMAQLVKAPFIKVEATKYTEVGYVGRDV
                     ESMIRDLVTSAVNMVRREQVETNRVQAEKLAEEALLQILLPSPPGIQPDPSQDPAQDG
                     SDPLAHAREKMRAKLRSGELDERIVEINLVQPQGSAEIFVGSGVEEIDVGLQNMISSM
                     LPQKSKLRRVAVREAKGLLIQQEAEKMLDMDSVASEAVRRVEQNGVVFIDEIDKISGR
                     ESGHGPDVSREGVQRDILPIVEGSTVNTRFGPVRTDHILFIAAGAFHVSRPDDLIPEL
                     QGRFPIRVTLQSLRHEDFKRILTKPKNALIKQYEALLATEGVHLKFEAAAIDELARVA
                     EECNRVSENIGARRLHTLLEQLLSEILYSASEKAGEKIVVTREYLKQRLHDTVRVNDL
                     GGYIL"
     gene            complement(2193..>2292)
                     /locus_tag="PLD82_08905"
     CDS             complement(2193..>2292)
                     /locus_tag="PLD82_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013385487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HOG65538.1"
                     /translation="DLDAPAIARKAMGIASEICIYTNANITIEELA"
BASE COUNT          453 a          703 c          652 g          484 t
ORIGIN      
        1 cggacttcga ttttttgggg aacgcagatc atgaccgccc cgcttttctc gagcgggccc
       61 atctcctgac agagtctgtc aggacaatgc gtctcgcgga tggaaacctg gccgcccttg
      121 atctccacca aaaacgcccc ggcagagcct tcaaagcgga gttccttggc ggccgggaga
      181 gtgtagcgcc agacaccttg tggtgtcgtg atccggacag cagaaggacc atcgccagtc
      241 tcccgggaaa agagaaagac cgccagggcc aacggtacaa gcacgatcag aagatcggcc
      301 ggggtcagat tcgctcggac agggggcatt acgaaaagcg cattccattg agcagggcat
      361 ccaaaaatcc ctgggccgca tccagggcag catcatcatc cagaccattg gccgcctgtt
      421 ccagcgtcaa gacacagatc tgctgacgca gattgatcac tgtcaacagg atccgcccgg
      481 cgggagcagt cacccgggca ataaccccgg cccttcccca catcatcatt tggccccgcc
      541 cgaggatgga tgcccccttg atcgcgccga tccgctcttc gagaatggcc gtgcaatctt
      601 tccctgcatc caggatttcc agtgcggccc ggattccggt cttcccgtca tggatggaga
      661 gagtataggg ctgaaccgat gatccggtaa accgggccgt ggacggaatg gtcaactcga
      721 atccccggct ctctgcggac caggtatccg tcgttccctg acggaggccg tccctgtggg
      781 ccgtacaccc ggcccagact gcgagaatga tcatgaggtg gagggacttc acaggatgta
      841 gcctccgaga tcgttgaccc ggaccgtatc atgaagccgc tgcttgagat actcccgggt
      901 gaccacaatc ttctctccgg ctttctccga agcagaatag aggatctccg agaggagctg
      961 ttcaagaaga gtgtgtagac ggcgcgcacc gatgttttcc gaaactcggt tgcactcttc
     1021 ggcgacccgg gccagctcgt caatcgcggc cgcctcgaac ttgagatgca ccccctcggt
     1081 tgcgagcaag gcctcgtatt gcttgatcaa agcgttcttc ggtttggtca gaatccgttt
     1141 gaaatcctca tggcgcaggg actggagggt gaccctgatc gggaaccgtc cctgcaactc
     1201 cggaatcagg tcatccggac gggagacatg gaaggcgccg gccgcaatga agagaatgtg
     1261 atcggtccgg accggtccga accgggtatt gacagtactt ccctcgacga tggggaggat
     1321 atcccgctgg acaccttccc gtgagacgtc cgggccatgc ccgctctccc gtcccgagat
     1381 cttgtcgatc tcgtcgataa acacgacccc gttctgttcg acccgacgga cggcttccga
     1441 cgcaacactg tccatatcca gcatcttctc ggcctcctgc tggatcagga ggcctttggc
     1501 ctcgcggact gcaacacggc gcagcttgga cttttgggga agcatcgagc tgatcatgtt
     1561 ctgcaggccg acatcgatct cctcgacgcc gctccccaca aagatctccg cactcccttg
     1621 cggctggaca agattgattt cgacaatccg ttcatccagt tcaccggatc tgagcttggc
     1681 gcgcatcttc tccctggcat gcgccagggg atcactcccg tcttgagccg ggtcttgaga
     1741 cggatccggc tggattcccg gaggcgaagg cagaaggatt tggaggaggg cttcttcggc
     1801 cagtttctcg gcctggaccc tgtttgtctc gacctgctca cgccggacca tattgacggc
     1861 actcgtcaca agatcccgga tcatggattc gacatcacgc ccgacatacc ccacctcggt
     1921 atactttgtc gcctcgacct tgatgaaagg tgccttgacg agttgtgcca tccggcgggc
     1981 gatctccgtc ttcccgacgc cggttggtcc gaccatcagg atattttttg ggaggatctc
     2041 gtcccgcatg gcaggatcga ccgccatccg gcgagagcgg ttccggacgg cgactgccac
     2101 ggcacgtttt gcgtcatcct gccccatgat gtatttgtcg agttcactga cgatttcctg
     2161 gggcgtcatc cccgttatca aaggattgct gttcatgcca gctcctcaat cgtgatgttg
     2221 gcgttggtgt agatgcagat ctcggatgcg attcccatcg ctttccgggc aatggccggg
     2281 gcatcaagat cg
//
LOCUS       DAOSHE010000365         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46242_length_2292_cov_3.142155, whole genome shotgun sequence.
ACCESSION   DAOSHE010000365 DAOSHE010000000
VERSION     DAOSHE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46242_length_2292_cov_3.142155"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLD82_08910"
     CDS             complement(<1..874)
                     /locus_tag="PLD82_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65539.1"
                     /translation="MQRSGRGLEIVKQGLKRPWCRLVFLCTGLFLGCTAILAAQSATS
                     SVQASSAGATNTARTNSSGKTNIVYNPQNEDERRTFMLKAFVENADDLLIRDVVRMFK
                     LPENGDLRELREQLFRYLGLVAVVQDPRKQAEVLIQDVFRSPDLNNNFVVQADEGVHV
                     RSTKDANAGLITLRGDNGNLRIRFKDQTISARLIRLDMKRREVYGEGNATIREKDRVI
                     VGDKFYFNTDSKYGVIYNAQTFMAPYYYFGKKIKKVGEKNYILENGWFSTCDADPPHF
                     GFGVGKAWLYQDTRI"
     gene            1035..2066
                     /locus_tag="PLD82_08915"
     CDS             1035..2066
                     /locus_tag="PLD82_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65540.1"
                     /translation="MPSFLIRLILLILPVLALSACGDADADQSDVLYAGSQEDENGNE
                     IPCYWVSGNITRLTTGNSRTGVARAALYDGDTLWIGGSEGDSSIPVLWKNGERIALPV
                     PSGATGFVSGLAPSMAGVIACGTIRSDGKSIPCFWRDYALVTNALEPTYSEASCIAIN
                     GQTMVLGGKYASTLPCIWTNDTRVKLEYTVPEGGMVSAVLFSARGEILAGGTCRTATG
                     SRACIWKNGTMTLLESSPGILSEVSGATLHEGQLVTCGSEAQIGNDVYAAYWVETAPR
                     LLPSTWAGSTNQVRAYANAVASWNGAIVVGGSIRDSLHGKLACTWVSGERVIIGKKNS
                     TVNAIAIRR"
     gene            complement(2075..>2292)
                     /locus_tag="PLD82_08920"
     CDS             complement(2075..>2292)
                     /locus_tag="PLD82_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65541.1"
                     /translation="VIEHNLDVIKRADWLIDMGPEGGRRGGIVVASGTPETVAASPES
                     HTGRYLAPLLGTAASAPKKKSSTRSRG"
BASE COUNT          478 a          689 c          659 g          466 t
ORIGIN      
        1 cgatccgtgt atcctgatac agccaggcct ttccgacacc gaatccgaaa tgcgggggat
       61 cagcatcaca ggtcgaaaac cagccgtttt cgaggatata attcttttcg ccgaccttct
      121 tgatcttttt cccgaagtag tagtagggag ccatgaatgt ctgggcatta tagatcacac
      181 cgtatttgga atccgtgttg aagtagaatt tgtcaccgac aatcacccgg tctttctccc
      241 ggatggtcgc attcccttcc ccatagacct cgcgccgctt catgtccagt ctgatcagac
      301 gtgcggagat tgtctggtcc ttgaagcgga tccgcaggtt tccgttgtcc ccgcgcagcg
      361 tgatcagacc ggcattggca tccttggtcg aacggacatg gacaccctca tccgcctgaa
      421 ccacgaaatt gttgttgaga tccgggctgc ggaagacatc ctggatcaga acctcggcct
      481 gttttcgggg atcctggacg acagcgacga gtcccagata ccggaaaagc tgttcccgca
      541 actcgcggag atccccgttt tcgggaagct tgaacatccg gacaacatcg cggatcaaga
      601 ggtcgtccgc attctccaca aaggctttga gcatgaaggt ccgtcgctcg tcctcgtttt
      661 ggggattgta cacgatgttg gttttgccac tggaattggt gcgggcggtg ttggtggcac
      721 ccgcagacga agcctggacc gaagatgtgg cagactgggc agcgaggatg gctgtacaac
      781 caagaaagag accggtgcac aggaagacaa gccgacacca cggtctcttc agtccctgtt
      841 tcacgatctc cagcccccgt cctgacctct gcatgcaatg gacaccccgc cctgtgtccc
      901 ggtcatgaaa atatcgccag aatcctttgg tcccgtccac attctcgcga caggaaggga
      961 aatccctcgg gacgggattt ttcagggact tgtgctacct ttcagggcgt acacccctgc
     1021 cgacggagag agacatgcca tccttcctga ttcgcctgat tctgctgatc cttcctgtcc
     1081 tggctctgtc cgcctgcggg gatgccgatg ccgaccagag tgacgttctc tacgccggga
     1141 gccaggaaga cgaaaacgga aacgagatcc cctgttactg ggtatccgga aacatcaccc
     1201 gattgacaac cggaaattcg agaacagggg tcgcacgggc cgcactctat gatggagaca
     1261 cgctctggat cggagggagc gagggcgact cgtcgatacc ggtcctctgg aaaaacggcg
     1321 agcggatcgc actcccggtt ccatccggtg ccacgggttt tgtgagcggg ctggcaccct
     1381 ccatggccgg tgtgatcgcg tgcggaacca tccgtagcga cgggaaaagc atcccgtgct
     1441 tctggcggga ctacgcactc gtgacaaacg ccctcgaacc aacatatagc gaggcctcct
     1501 gtatcgccat caacgggcag acaatggtcc tgggaggcaa gtatgccagc actctgccgt
     1561 gtatctggac caacgatacg cgcgtcaagc ttgaatacac agtcccggag ggcgggatgg
     1621 tctctgcggt cctcttttcg gcccggggcg agatcctggc cggcgggacc tgccgaaccg
     1681 caacgggaag ccgagcctgc atctggaaga acgggaccat gaccctgctt gaatcgtctc
     1741 ccggcatcct gtccgaagtc agtggcgcca ccctccatga aggccagctg gtgacctgcg
     1801 gatcggaagc acaaatcggg aacgatgtct acgccgccta ctgggtggag acagcacccc
     1861 gtctcctgcc ctcgacctgg gccgggtcaa ccaaccaggt gagagcctac gccaacgccg
     1921 ttgcctcgtg gaacggagca atcgtcgtcg gcggctccat cagggacagc ctgcacggaa
     1981 aactggcctg cacctgggtc tccggcgagc gggtcatcat cggcaaaaaa aacagtaccg
     2041 tcaacgccat cgccattcgc cgctgatcgc gctttcagcc tcttgagcgg gtactggact
     2101 tctttttagg tgcggaggca gcagtcccga ggagcggggc cagatagcgt ccagtgtgtg
     2161 attcagggct ggccgcgaca gtctccgggg ttcccgaagc gacaacaatc cctccccgtc
     2221 tcccgccttc ggggcccata tcgatcagcc agtcggcacg cttgatgacg tcaaggttgt
     2281 gctcgatcac ga
//
LOCUS       DAOSHE010000366         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46626_length_2278_cov_3.875843, whole genome shotgun sequence.
ACCESSION   DAOSHE010000366 DAOSHE010000000
VERSION     DAOSHE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46626_length_2278_cov_3.875843"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PLD82_08925"
     CDS             <1..1090
                     /locus_tag="PLD82_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG65542.1"
                     /translation="VNPLHHTNSSAITDWGLRRANTRIHLFSRYSTPWWKALQEIADR
                     APGRVVAPVVARHFDFLEELAGSGRLGTDPADLAESFSKIDFLIGSAPVGPTTVERVQ
                     RLTGRLPVVRFGSTETCLQVLGTPVALAPGHRLRIFADGWEHRRNDVPETGYWIGREH
                     PGLTECRIVRAIDPGQPGFMEDLPDGVPGYLVTRGRNLMKGYVNDDARTAAVFKDGWY
                     LGLEDICFALTDPADGSRNFYWMSRDSALIIRGGSNYSCEQINEELRTWTVARYGLHP
                     DEFILASAGIKIGSEHEDACCVTIELLTDHARSLQDEIQGSFIPESRAQVSKGAKPDR
                     LRFAPVPVNFKGAILLAELKKAWENNRD"
     gene            1230..>2278
                     /locus_tag="PLD82_08930"
     CDS             1230..>2278
                     /locus_tag="PLD82_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65543.1"
                     /translation="MGEQHTDVGTCQPKSRMTPDGEAVLALARSVETRLSGSENGFLV
                     LGQALEKIVCTSNALHDGMAQQSSSLRSGQGADLINRLESASIIPLDEHDRIRKILVA
                     DLELIERISAEIADLPKAALLLGALVKRLHIIALNSLITATRAGSAGQRISQFAGDVR
                     MLGKRLADLSAEFSLSAETGARQQDIRRDALHARIREEERIAEAEQAAAHRTMEAVRS
                     LGLNVQTTLDRVVGYAATLTAQSMDIITHLQIHDIVRQRLEHIVEALRNAPSDPAVLG
                     LQHAQMRDTIRQLNAAIPGITRAFSTIGQALDDLRARLAAEQMQEMLHALDMMGESIT
                     GLDGLAESQRTIIVE"
BASE COUNT          474 a          696 c          659 g          449 t
ORIGIN      
        1 cgtcaatccg ctccaccaca caaattcttc cgccattacc gattggggcc tgcgccgggc
       61 caacaccaga atccatctct tttctcgcta ttcgacacca tggtggaaag cgctccagga
      121 aatcgccgac agggcacccg gcagggtggt ggccccggtc gttgcccgcc attttgactt
      181 tcttgaggaa ctcgccggct caggccgcct gggcactgat cccgcggatc tggccgaatc
      241 cttttcgaaa atcgatttcc tgatcggttc agctccggtc gggccgacga cagtcgaacg
      301 ggtccaacgg ctcacgggaa ggctcccggt cgtccgattc gggtcgaccg agacctgtct
      361 ccaggttctt ggcacaccgg tcgcccttgc gcccgggcac cggctccgga tctttgccga
      421 tggctgggaa caccggcgga atgatgtccc cgagaccggg tactggatag gacgggaaca
      481 tcccggcttg accgaatgcc gtattgtcag ggcgattgat cccggacagc ccggtttcat
      541 ggaagacctg cctgacgggg tcccgggata tctcgtcacc aggggaagaa acctgatgaa
      601 gggctacgtc aatgacgatg ccaggacggc tgctgtgttc aaggatggct ggtatctcgg
      661 actcgaggat atctgtttcg cgttgaccga tcccgctgac gggtccagaa atttttactg
      721 gatgagccgg gattcagccc tgatcatccg cggtggctcg aactactcct gcgaacagat
      781 caatgaagag ttgcggacat ggacggtggc acgctacgga ctccatcccg acgagttcat
      841 cctggcatcc gccggaatca agatcggaag cgagcacgag gatgcctgct gcgtcacgat
      901 cgagcttctc acggatcatg cccgcagtct tcaagacgag atccaaggga gctttattcc
      961 tgaatcccgt gcccaagtct cgaaaggagc caagccggac aggctccgct tcgcaccggt
     1021 tccggtcaat ttcaagggtg ccatcctcct cgccgagctg aaaaaggcct gggaaaacaa
     1081 tcgggactga ccgggtatct gcctgactcc aagcgggaca aagcgtgctt ttattgcaaa
     1141 aaatcagtat tcctacgtat tgcgacagtc catcttcccg ggtatagttg ggtatcgagc
     1201 gttgctgctc ctggaggatt cctgtcgtta tgggcgaaca gcacacggat gtcggcacct
     1261 gccaaccgaa gtcacggatg acgcccgacg gtgaagctgt gctcgctctt gcccggtcgg
     1321 tcgaaacccg cttgtccggc tcggaaaacg gctttctcgt cctcgggcaa gccttggaga
     1381 aaatcgtctg tacctcgaat gcccttcatg acggaatggc gcagcagtca tcgagcctgc
     1441 gcagtggcca gggcgccgat ctcatcaacc ggctcgaatc ggcttccatc atcccgctgg
     1501 acgagcatga ccggatccgg aagatcctgg ttgcggatct tgagctgatc gagagaatct
     1561 cggccgagat tgccgatctg cccaaggcag cgctcctcct cggtgccctt gtcaagcggc
     1621 tgcacatcat tgccctcaac tccctgatca cggcaacccg ggccggttcg gccggacaga
     1681 ggatctcgca atttgccgga gatgtccgca tgctcggcaa gcggttggcc gacctgagtg
     1741 ccgaattctc gctcagtgcc gagacaggcg cccggcagca ggatatccgc cgggatgccc
     1801 tccatgcgag aatcagggaa gaggaacgga tcgccgaagc cgaacaagcg gccgcacacc
     1861 gcaccatgga agccgtccgc tctctgggcc tgaacgtcca gacaactctg gaccgggtgg
     1921 tcggatatgc ggccacactg acagcacaat ccatggacat cattacacat cttcagattc
     1981 atgatattgt gcgccaacgg ctggaacaca tcgtcgaagc tctccggaat gcgccttcgg
     2041 atccggccgt cctcggtctc cagcacgccc agatgcgtga caccatccgg caactcaatg
     2101 ccgccatccc cggaatcacc cgggccttca gcacgattgg acaggctctg gatgacctgc
     2161 gtgccaggct tgccgccgaa cagatgcagg agatgctcca tgcccttgac atgatgggag
     2221 aaagcattac gggactcgat ggtctggcgg agagtcagcg gaccatcata gtggaaat
//
LOCUS       DAOSHE010000367         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46636_length_2278_cov_2.830859, whole genome shotgun sequence.
ACCESSION   DAOSHE010000367 DAOSHE010000000
VERSION     DAOSHE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46636_length_2278_cov_2.830859"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="icd"
                     /locus_tag="PLD82_08935"
     CDS             complement(<1..1051)
                     /gene="icd"
                     /locus_tag="PLD82_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOG65544.1"
                     /translation="MENTIIRFENGSLLVPDRPVIPFIEGDGTGPDIWAASVRVMDAA
                     VKKAYNGKRAVEWKEVLAGQKAFDRTGSWLPDETIEAFRTYRVGIKGPLTTPVGGGIR
                     SLNVALRQILDLYVCLRPVKYFEGVPSPVKRPADVDMVIFRENTEDVYAGKELEEGSD
                     KAKKLIEFLKAEFGWDLRPDSGLGLKPVSRTGSERLIRAAIRHAIEHGLPSVTLVHKG
                     NIQKFTEGAFRKWGYELAEREFGGQTVSWEACNGNPPAGRILIKDCIADNFLQQILTR
                     PREFSVIATTNLNGDYISDALAAEVGGIGIAPGGNINYESGFAVFEATHGTAPKYAGQ
                     DKVNPGSVILSGEMML"
     gene            complement(1164..1517)
                     /gene="speD"
                     /locus_tag="PLD82_08940"
     CDS             complement(1164..1517)
                     /gene="speD"
                     /locus_tag="PLD82_08940"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOG65545.1"
                     /translation="MAQTLGTHLLLEFSGCPFALLDDPATVERALLAAAAAAGTTVLE
                     AHFHRFQPQGVTGILLVSESHLSIHTWPELGYAAVDFFSCSPKTRFAAAEQVLQQAFT
                     PARIARQVVERGPEG"
     gene            1623..1853
                     /locus_tag="PLD82_08945"
     CDS             1623..1853
                     /locus_tag="PLD82_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65546.1"
                     /translation="MAGERRVGMVILVCVLGALGGVVFGRLLVVLLPGLDVVFRPGID
                     LGFDIHVLRAGIRFNIAAVLGIVAALLVWRRL"
     gene            1850..>2278
                     /locus_tag="PLD82_08950"
     CDS             1850..>2278
                     /locus_tag="PLD82_08950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG65547.1"
                     /translation="MNTLPRVVLASQSPRRLALLRSIGIEPLVQPAFLDEQMLAGEDA
                     WQLVRRLAHGKVAAVMARGLDVSPALLLGADTVVVLDGAVLGKPGNTDAALDMLQRLS
                     GREHRVVTGLALMCLPDGVLDVSLAETVVRFRALDQEEICA"
BASE COUNT          373 a          643 c          738 g          524 t
ORIGIN      
        1 gcagcatcat ctcgccggag aggatcactg agcccggatt gaccttgtcc tgaccggcat
       61 attttggagc cgttccgtgg gttgcctcaa agacggcaaa cccgctttcg tagttgatgt
      121 tgcctcccgg ggcgatcccg attccgccga cctcggcagc cagggcgtcc gagatatagt
      181 cgccgttgag attggtcgtg gcaatgaccg aaaactccct cgggcgggtc aggatctgct
      241 ggaggaagtt gtcggcgatg cagtccttga tcaggatcct gcctgcgggc ggatttccgt
      301 tgcaggcctc ccaggagacc gtctgcccgc cgaactcgcg ttcggcgagt tcgtagcccc
      361 acttgcggaa agcgccttcg gtgaatttct ggatattgcc cttgtggacc agggtgacac
      421 tggggaggcc atgctcgatt gcatgccgga tcgcggcccg gatcaggcgc tcgcttcccg
      481 tgcgcgaaac cggcttgaga cccagtcccg aatcgggtct cagatcccag ccaaactcgg
      541 ccttgagaaa ttcgatcagc ttttttgcct tgtcgcttcc ctcttcgagc tccttgccgg
      601 catagacgtc ttcggtgttt tcgcgaaaaa tgaccatatc aacatcggcg ggacgcttga
      661 cgggcgaggg gacaccctca aagtacttga cgggtcgcag acagacatag aggtcgagga
      721 tctggcgcag ggccacattg aggctgcgga tgcccccgcc gaccggtgtt gtcagcgggc
      781 ccttgatccc gacccggtag gtgcgaaacg cctcgatggt ctcatcggga agccagcttc
      841 ctgtccggtc aaaggctttc tgtccggcca ggacttcctt ccattcgaca gctcttttcc
      901 cgttgtatgc ctttttgaca gctgcatcca tgacgcgcac actggcagcc cagatatccg
      961 gcccggtccc gtcaccttcg ataaaaggga tgaccggtct gtccggcaca agcaggctgc
     1021 cgttttcgaa acggatgatc gtgttttcca tagggtggtt gtttccttca gacgtttgtg
     1081 gaatcgttct gccgcagggc agatcccgca gataatagta gtttggactt cttgtacagg
     1141 gcaagcacgc ctgtccggct ggtttacccc tccggccctc tctcaacgac ctgccgggca
     1201 atccttgcag gggtgaaggc ctgctgcaaa acctgctcgg ctgcagcaaa gcgggttttc
     1261 gggctgcagc tgaaaaaatc gaccgctgcg tagccgagtt cgggccaggt atggatgctc
     1321 aggtgggatt cgctgacgag aaggatgccg gtgaccccct ggggctggaa gcggtgaaaa
     1381 tgggcctcaa ggacagtggt tccggccgcg gctgcagccg cgaggagggc ccgctcgact
     1441 gtggccggat catccagcag ggcaaagggg cagcccgaaa attcgaggag gaggtgtgtg
     1501 ccaagtgtct gagccatgat gcgttcaaga ataacacata tcgcggttcg tgtccggctt
     1561 gccgatcctt ttgcctgtgc gggcgacggg tactatgttt ttttgtgcag ggagggtgtg
     1621 tcatggcagg tgagcggcga gtcgggatgg tcatccttgt gtgtgtgctt ggagccctgg
     1681 gcggtgttgt cttcgggagg ctgcttgtgg tcctcctgcc ggggctggat gtcgtgttcc
     1741 ggcccgggat tgatctgggg tttgacattc acgtcctgcg ggccggtatc cgcttcaata
     1801 ttgcggcggt cctggggatt gttgctgccc ttctggtctg gaggcggttg tgaacacact
     1861 cccccgggtc gtgcttgcct cgcagtcgcc gagaaggctt gcgctattgc gctctatcgg
     1921 aatcgagcct cttgtccagc ctgcgtttct tgatgaacag atgctggctg gtgaggatgc
     1981 gtggcagctt gtgcgtcgcc ttgcccacgg caaggtcgcg gctgtcatgg ccagggggct
     2041 tgacgtttcg ccggcgcttc tcctcggggc cgacacggtg gtcgtgcttg acggggctgt
     2101 cctcggaaag cctggcaaca cggatgcggc cctggacatg ctccagcggc tttcgggccg
     2161 ggagcaccgg gttgtgaccg gcctggccct gatgtgcctg cccgatggtg tccttgatgt
     2221 ctcgctggcc gaaaccgttg tccgcttcag ggcgcttgac caggaagaga tttgtgcg
//
LOCUS       DAOSHE010000368         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46660_length_2277_cov_3.084608, whole genome shotgun sequence.
ACCESSION   DAOSHE010000368 DAOSHE010000000
VERSION     DAOSHE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46660_length_2277_cov_3.084608"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLD82_08955"
     CDS             complement(<1..675)
                     /locus_tag="PLD82_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG65548.1"
                     /translation="MNLELYEGEVHALLGENGAGKSTLMNMLAGIYTPDRGHIEINGE
                     STVFTSPADAIRHGIGMIHQHFKLVEAMTALENILVGEKKGFFLDKSKRRERVLGVAA
                     RFGLSVDLDRRVYEMSVGEKQILAILQVLSRGARVLVLDEPTTVFTPQETKNLFAIIR
                     RMKDEGCTIVFISHKMDEVMEISDRITVLRKGETIQTLDKHHTNPHHLTELMVGRAVD
                     LSIERAK"
     gene            complement(842..1921)
                     /locus_tag="PLD82_08960"
     CDS             complement(842..1921)
                     /locus_tag="PLD82_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG65549.1"
                     /translation="MQRVFAVMIAVVLALGLVAVGCSKSGAKSDKIKVGFIYIGPIGD
                     GGWTYAHDEGRRFLEKETGIESSYKENVPESSAAEKVMEDMISQGCNIIIGTSFGYMD
                     YMENVAKRHPDVTFLHCSGYKTGKNMGTYFGRIYQARYLSGIVAGLKTKANKIGYVAA
                     YEIPEVVRGINAFTLGVRSVNPQAVVKVKWTHTWYDPAKEKDAAKALLDEGVDVVAQH
                     QDTAGPQQAAEERNVWSIGYNTDMNSKAPKAYMTAPIWNWGPYYVRQIKAVKEGTWKS
                     ESYWGGMNDDIVRLAPLTANAPEAAKAAVDKATKDILAGTQKVFQGPIKDQTGAVKIK
                     DGEVMADADMLKFDWFVEGVEGKIK"
     gene            complement(2044..>2277)
                     /locus_tag="PLD82_08965"
     CDS             complement(2044..>2277)
                     /locus_tag="PLD82_08965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG65550.1"
                     /translation="ILEEIQNRDVVVDIGNRILAAIGEPVRVDDYDVSVTGSIGIALW
                     PADAEDSGELLVRADRAMYVAKRGGKNTMTFSG"
BASE COUNT          488 a          670 c          566 g          553 t
ORIGIN      
        1 ctttgcccgc tcgatcgaaa gatccacggc acgacccacc atcaattcgg tgagatgatg
       61 ggggttggta tggtgtttgt ccagggtctg gatcgtctca cctttccgga ggacagtgat
      121 ccggtccgag atttccatga cctcgtccat cttgtggctg atgaaaacga tggtgcaacc
      181 ctcatctttc atgcgcctga taattgcaaa cagatttttc gtttcctgtg gtgtgaacac
      241 ggtcgtcggt tcatccagca caagaacacg cgccccgcgc gaaagcacct ggagaatggc
      301 caggatctgc ttctctccga ccgacatctc gtagaccctg cggtcaaggt cgacactgag
      361 accgaagcgg gccgcaaccc cgagcactct ctcccggcgt ttgcttttat cgagaaaaaa
      421 cccctttttt tcaccgacca ggatgttttc aagggctgtc atggcctcca ccagcttgaa
      481 atgctgatgg atcataccga tcccgtggcg aatggcatcc gcgggcgagg taaacacggt
      541 cgactccccg ttgatctcga tatgtccgcg gtcgggagta tagatccccg caagcatgtt
      601 catgagcgtg ctctttcccg ctccgttctc accgagcagg gcatgcactt caccctcata
      661 cagctcaaga ttcacatcat gattcgcaac aactttgcca aataccttgg aaatgcccct
      721 catagcgaca caaatgcgct gcgtttccat aggccaggtt cgctccattt ctttgtctgc
      781 gggtctccat aaaaaggccg cgtgcatcca ctgcccgcgg ccccggaaat gcaaagcgat
      841 cttattttat cttgccttcg acaccttcga caaaccagtc gaacttgagc atatcggcgt
      901 ctgccatgac ctcgccatcc ttgatcttga cggcaccggt ctggtccttg atcggtccct
      961 ggaagacctt ttgggtcccg gccaggatgt ccttggtggc tttatcaact gcggccttgg
     1021 ctgcctcggg agcattggcc gtcaacggtg ccaagcggac aatatcgtca ttcatcccac
     1081 cccaatagct ttcggacttc caggttccct ctttgaccgc cttgatctgg cgcacataat
     1141 acgggcccca gttccagatc ggagccgtca tgtaggcttt gggagccttg ctgttcatgt
     1201 ccgtgttata tccgatcgac cagacattgc gctcttcggc cgcctgctgc gggccggctg
     1261 tgtcctggtg ctgggcgaca acatcgacac cctcatcgag cagagctttg gctgcatcct
     1321 tttccttggc cggatcatac caggtatggg tccacttgac cttgacgaca gcctggggat
     1381 tgacggagcg gacaccgagg gtgaaggcgt tgatgccacg aacgacctcg gggatctcgt
     1441 aagcggcaac gtatccgatc ttgttggcct tggtcttcag tccggcaaca atacccgaga
     1501 gatagcgggc ctggtagatg cgaccaaaat aggtccccat gttttttccc gtcttgtatc
     1561 cggagcaatg gaggaaggtc acatcgggat ggcgtttggc aacgttttcc atgtaatcca
     1621 tgtacccgaa gctcgttccg atgatgatgt tgcagccttg ggaaatcatg tcttccatca
     1681 ccttttcggc agctgacgac tccggaacat tttccttgta actgctttct atgccggttt
     1741 ctttttcaag aaaccgcctg ccctcatcgt gggcataggt ccagccacca tctccgatcg
     1801 ggccgatata aataaatccg accttgattt tgtcactctt ggccccggac ttggagcaac
     1861 cgacagcaac caatcccagt gccagaacca cggcaatcat gactgcaaac actctttgca
     1921 tgaacaacct cccgaacgtt gtgtctagta cctggtcaca cacaggctcc cgtcagggta
     1981 taccgaggga gaagattctg tcaagaaagt tcggaaaatt tgctggatat gcttgtgtgc
     2041 agatcaacca gagaaggtca tggtgttctt gccaccacgc ttggctacat acatcgcccg
     2101 atcggccctg acgagaagct cccccgagtc ttcggcatca gcaggccaga gagcgattcc
     2161 gatactgcca gtcaccgaaa catcatagtc atcaacacgg acaggttctc cgatcgcggc
     2221 cagaatccgg ttcccgatat caacaacgac atcccgattc tggatttcct cgagaat
//
LOCUS       DAOSHE010000369         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46662_length_2277_cov_2.866337, whole genome shotgun sequence.
ACCESSION   DAOSHE010000369 DAOSHE010000000
VERSION     DAOSHE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46662_length_2277_cov_2.866337"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1910)
                     /locus_tag="PLD82_08970"
     CDS             complement(315..1910)
                     /locus_tag="PLD82_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65551.1"
                     /translation="MPADENAAFRLSVLYDLSLYISGCKDPVEILAGIVRHLRAVMPI
                     EYAWLIIRRGDAFAELAVLGEDGETVLPGTRPVDNTIIESIVMQEFDGVVPDLPRWKQ
                     HNEDGFFPEGAGSAVICPVQRGKDADGCLIVASRRKEAYGRDQALLLFMLALQLGIVL
                     RSLQLRQALEEQNARLAAIFDTLDEGLVLIDNWGSMMTANPALTRTIFPGTEPDRGQS
                     WYAWMLGQKERFTPDRWLPLAETITGLADGDFPVPFVMEERGGKPGQPVLRGEYHLAG
                     GSGSTRRILVILRDARETYRAEALRRDLTSMLVHDLRSPLGIINWNMELMLDGVLGEV
                     TAEQERFLKGSIESTQELLDMIDSLLDIDRLETGALELDRSEIDLAVLAAEIAQRMDF
                     VSHQLKLSFELRFEAGFPRVVADRQLVRRVLFNLFFNASKYAPQGSTIHVSGSSTPDW
                     VSIAVEDEGKGIPEKYLDLIFDKYVQAEARNRGEIKGKGLGLTFCRLAVEAHGGRIRA
                     ENARGARFVFELPREGNVAAPKG"
     gene            complement(1900..>2277)
                     /locus_tag="PLD82_08975"
     CDS             complement(1900..>2277)
                     /locus_tag="PLD82_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65552.1"
                     /translation="IESRLAAPFHQDNLFEEPEIRDVLEAAGSFIDQRIRGEAVLTVG
                     KAVDFIHRGFAGIVNVMPFSCMPGLIVSALAPAIRERYHDIPWLNLVFEDSASDIDQM
                     RLEAFVEQARAWQEQFGRRKHAR"
BASE COUNT          455 a          739 c          674 g          409 t
ORIGIN      
        1 gtcaacctgg cctgcgggat gcgcatggtc cataccaatg aggaatacgt cgtggttgag
       61 gatctggtca aggcggccgg cctggtcact gccattctcg ccgacaaggt caaaattttg
      121 gggaacagcc tcacgccctg aggagactcg gcctgacgga gaccgggggt gggtgcgggc
      181 cgggcagcac ggcctgtccc tactccgtta tccggagggt cgtgcatccg gggaagccgg
      241 cgagacagag cggaatcacc ctctcccgtc catcccccat ccgggttccg gacggctgtc
      301 ctgcgccggt tggctcaccc cttgggggcc gccacgtttc cttcccgggg aagctcgaag
      361 acaaaccggg cgccacgggc attctcggcg cggatccgcc cgccatgggc ctcgacagcc
      421 agcctgcaga acgtcagccc taaccccttg cccttgatct ccccgcgatt gcgggcctcg
      481 gcctggacat acttgtcgaa gatcagatcc aggtattttt ctgggatgcc ctttccttcg
      541 tcctcaacgg caatcgagac ccaatccggt gtcgaactcc ccgaaacatg gatcgtactg
      601 ccctgtggcg catactttga cgcgttgaaa aagagattga agaggacccg ccgaacaagc
      661 tgtctgtcgg caaccacccg gggaaaaccc gcctcgaacc ggagttcgaa cgagagcttg
      721 agctggtggg agacaaaatc catgcgttgc gcaatctccg ccgccaggac ggccagatca
      781 atctcgctcc ggtccagttc gagggctcca gtctccaggc gatcaatgtc gagaagcgag
      841 tcgatcatgt caaggagttc ctgcgtactc tcgatggaac ccttgaggaa gcgctcctgc
      901 tccgctgtca cctcgcccaa taccccgtcc agcatcaact ccatattcca gttgatgatc
      961 ccgagcgggg agcgcaaatc gtggaccagc atggaggtca ggtcccggcg gagtgcttcg
     1021 gcgcgatagg tctcgcgggc atccctcagg atgacgagga tcctgcgcgt cgagcccgag
     1081 cctccagcca gatggtactc tccacggagg acgggctgtc cgggctttcc cccccgctct
     1141 tccatcacaa atggaacggg aaaatccccg tcagccagac cggtgatggt ttcagccagg
     1201 ggaagccagc ggtcgggcgt gaaacgctct ttctgaccga gcatccaggc gtaccaggac
     1261 tgcccccggt ccggctcagt gccgggaaaa atcgtccggg tcagggccgg gttggccgtc
     1321 atcatgctcc cccagttgtc gatcagcacc agcccctcgt ccagggtatc aaagatcgcc
     1381 gcgagacggg cattctgttc ctcgagagcc tgtcggagct ggagggaacg caggacaatc
     1441 cccaactgca gcgccagcat gaagaggaga agggcctgat cccgcccgta ggcttccttc
     1501 cggcgactgg ccacgatcag gcaaccgtcg gcatccttgc cccgctggac gggacaaatc
     1561 accgcgctcc cggccccctc gggaaagaag ccgtcctcat tgtgctgctt ccagcgcggg
     1621 aggtccggca cgaccccgtc aaactcctgc atcacgatgg attcgatgat cgtgttgtcg
     1681 accggcctgg tgccgggaag gaccgtctcc ccgtcttcgc caagaacagc cagttcggcg
     1741 aaagcgtccc cccggcggat aatcagccag gcatactcga tcggcatgac cgcccgcagg
     1801 tgtcgtacga tccccgccag aatctcgaca ggatccttgc acccgctgat atagagggag
     1861 aggtcgtaga gtacggagag acgaaaagcc gcgttctcgt cagcgggcat gttttcgcct
     1921 cccgaattgt tcctgccagg cccgcgcctg ttcgacaaaa gcctcaagac gcatctggtc
     1981 aatatccgag gcactgtcct caaagaccag gttcagccag ggaatgtcat gatagcgctc
     2041 gcggatggcc ggtgcaagcg cactcacgat caagcccggc atgcaggaga acggcatgac
     2101 gttgacgata ccggcaaaac cgcggtggat gaaatcgacc gccttcccga ccgtcaggac
     2161 cgcttccccg cgtatccgct ggtcaatgaa actcccggcg gcttccagaa catcccggat
     2221 ctcgggttcc tcaaacaggt tgtcctggtg gaacggcgcg gccagccggg attcgat
//
LOCUS       DAOSHE010000370         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_46846_length_2271_cov_2.748646, whole genome shotgun sequence.
ACCESSION   DAOSHE010000370 DAOSHE010000000
VERSION     DAOSHE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46846_length_2271_cov_2.748646"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1576
                     /locus_tag="PLD82_08980"
     CDS             38..1576
                     /locus_tag="PLD82_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG65553.1"
                     /translation="MMILGSGLDRIKEKDFSSQITLASGDEFGDVAQTVNMMTENIRN
                     YTENLELLVEARTKELQMAFNEVHSLKVQQDGDYFLTSLLINPLLEYSVQTEKVFLEA
                     RISQKKKFRFRSHDGEIGGDVCIARQVSMHDKRYTAFVNGDAMGKSLQGAGGALVLSV
                     IFNAYVNRTHVFGTANHPYPEKWLRQCYNELQTVFTSFDGSMMMSVIVGLLDEESGML
                     YYFNAEHPYPVLYRGGIASFADCELSSQKLGSFGTIENLKLRTMQVESGDVLILGSDG
                     RDDILLGHDALTGARLINEDETLFLRFVEEGDGDLDKILDHLNKAGEITDDLSLMRIA
                     YTTPARMMPGDYAVRRDAAISMIHEGSVEQALNELRILIAEYPDRDAFKAAIGCCQRI
                     YRGLESEIRESDLGAGILRVQAEILEDAHIYFPLEKEYMYLRAYTAFQLGNTVAAHEY
                     AERFRLYYPEHVNNLVILAESALRLKKLHKAELVVGDIARFDPDNSNLDGFRQRILTA
                     REKA"
     gene            complement(1771..>2271)
                     /locus_tag="PLD82_08985"
     CDS             complement(1771..>2271)
                     /locus_tag="PLD82_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG65554.1"
                     /translation="DWSVDERQDYWRASEAAAKYLAGLYKQFGDWLLAAAAYNAGQNY
                     IKSRLEATGSTSFWELVEKDALWKETTHYVPRILAAMRIFRDPQRYGFTMDGASEPLT
                     VCRGRIAASTLVEASAICGVPVYRLIELNPHLERGVIPDDANGYELTLPHPGEFSDQD
                     DYTGMT"
BASE COUNT          549 a          654 c          610 g          458 t
ORIGIN      
        1 gctcttcttt ttcatctagc ggatggtcct tcaacccatg atgatcctag gaagcggcct
       61 cgaccggatc aaagagaaag atttttcaag ccagatcacg cttgcgagtg gcgatgagtt
      121 tggcgacgta gcccaaaccg tcaacatgat gaccgaaaac atccggaatt acaccgagaa
      181 cctggagctt ctggtcgaag cccggacaaa agaactgcag atggccttca acgaggtcca
      241 cagtctcaag gtgcaacagg acggtgacta tttcctgacc tcgctcctga tcaacccgct
      301 tttggaatac agcgtccaga ccgagaaagt cttcctcgaa gcacggattt cgcaaaaaaa
      361 gaaattccgc ttccgttcgc atgacggcga gatcggtgga gacgtctgca ttgcgagaca
      421 ggtcagcatg catgacaagc gatatacggc cttcgtcaac ggggatgcca tgggaaaatc
      481 ccttcagggt gcgggcgggg ctctggtatt gagcgtcatc ttcaacgcct atgtcaacag
      541 aacccacgtc tttggaacag ccaaccaccc ctaccctgaa aaatggctcc ggcaatgcta
      601 caacgaactc cagacagtct ttacgtcctt tgacggaagc atgatgatgt ccgtcatcgt
      661 cggactgttg gatgaggaga gcgggatgct ctactatttc aacgcggagc atccctaccc
      721 ggtgctctat cgcggcggga tcgcttcatt tgccgattgc gaactctcct cacaaaaact
      781 gggttccttc ggaacgatcg aaaacctcaa gctccggacg atgcaggtcg aaagcggcga
      841 cgtcctgatt ctcggatcgg atggccggga tgacattctc ctggggcacg atgccctcac
      901 cggcgcacgt ttgatcaatg aagacgagac actcttcctg cgctttgtcg aagaagggga
      961 cggagatctc gacaaaatcc tggaccatct caacaaagcc ggcgagatta cggacgacct
     1021 ctcgctgatg cgcatcgcat acacaacacc agccagaatg atgcccgggg actacgcagt
     1081 acgcagggat gcagccatca gcatgattca cgagggatct gtggaacaag ccctcaatga
     1141 gttgcgcatc ctgatcgccg aatatcctga tcgggacgcc ttcaaggctg ccatcggatg
     1201 ctgccagcgg atctatcggg ggctggagag cgaaatccgc gagagcgatc tgggtgcggg
     1261 cattctgcgg gtccaggccg aaatcctgga ggacgcccac atctactttc ccctggagaa
     1321 ggagtacatg tacctgcggg cctacacggc cttccagctc ggcaacactg ttgccgctca
     1381 cgagtatgcc gaacggttcc ggctgtatta ccccgaacat gtcaacaatc tcgtgattct
     1441 ggccgagtcg gccctccggc tgaaaaagct tcacaaagcg gaacttgttg tgggagatat
     1501 tgcccggttc gatccggaca attccaatct tgatggattc cggcaacgca ttctcacagc
     1561 gcgggaaaaa gcctagcgag tccggtacgt cagcgcagcg ggccggaaga cggcaggccg
     1621 acatcgatgc ttcccggaca agaggcaccc ggatggtacg aagaccggtc gagcagaatc
     1681 cgttcgacca accatcctgt atcatgtcga gaccgggata cataactccc gccaaccgga
     1741 cgtcgtccgt ctggctcgca gcccgggtgg tcaggtcatc ccggtataat catcctgatc
     1801 ggagaattcc ccaggatggg gcaaggtcaa ctcatacccg ttggcgtcat ccggaatcac
     1861 accccgttca agatggggat tgagttcgat caaacggtac acaggcacgc cacagatcgc
     1921 agaggcctca accagagtcg atgcggcaat ccggccccga caaaccgtca acggttcgga
     1981 agcgccatcc atggtgaatc cataacgctg tggatcccga aaaatccgca tggccgccag
     2041 gatccttgga acataatggg tcgtttcttt ccagagagca tctttttcaa caagctccca
     2101 gaatgacgtc gagcctgttg cttccaatct gctcttgata tagttctggc cagcattgta
     2161 ggcggctgcg gcgagcaacc agtccccgaa ctgcttgtac agaccggcca aatatttggc
     2221 ggctgcctcg ctggcacgcc aataatcctg gcgctcatcg acagaccagt c
//
LOCUS       DAOSHE010000371         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_47170_length_2260_cov_4.064399, whole genome shotgun sequence.
ACCESSION   DAOSHE010000371 DAOSHE010000000
VERSION     DAOSHE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47170_length_2260_cov_4.064399"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1814
                     /locus_tag="PLD82_08990"
     CDS             249..1814
                     /locus_tag="PLD82_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65555.1"
                     /translation="MVRALLTACACLVLAAAWAPAASRPDPGEGDRLAGAGKHADAVR
                     EYKKYVQTVGYSRESGRVLLALARSAHASGDLGSFVWARGLFTRWFPVEMGPVREFDE
                     YEASLRMRAGDWAGAMALVEGLLKKYSYPPEVGDRWRGLLIAGYDRLARPDRVFEIRL
                     ELVRSGRRTAQVADALYRVSEWALKKDKRELALYYVNRLLSEHPGWPHIRHAEGLKRA
                     ITWKTITVEDGLGDNSVSAIRFDGDDIWVGTWLGGVTRFSRSQGRLQKYTAKNSGLVS
                     DLIRDLCIDQTRVWTATFEGLAWFDKRTDAWQTVLTVAGLAGQRIKCVTVSDNRLWVG
                     TIGNGVSMLDFRNQKWRTWRRADGLPDENIVTITHTPGSVWAGSLAGGIARYDLVAEK
                     WKTWAAPMGAPLQNIKATAFDGQRLWIATHGAGLYSCDENGTGWEQYTSGNSGLPVDF
                     VHAVSVGPDGRIWAGTLEGGAAVFERRTGRWKVYTIHDGLAGNDVITIAFEGRYIWFG
                     TLNGGISVLLGDG"
     gene            1869..>2260
                     /locus_tag="PLD82_08995"
     CDS             1869..>2260
                     /locus_tag="PLD82_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65556.1"
                     /translation="MSEQETQPRTGDEGRQDRPLVLLAVAFFLSFLAGMFLVLRGEGM
                     TGSALPGSGPEQPGIAVIDIEGVIQYASQQGFMNPGGAGAPVVVETVRRMGENPLVRG
                     LVVRINSPGGSIGATQEIYHALRRFRESG"
BASE COUNT          413 a          620 c          772 g          455 t
ORIGIN      
        1 cctcccctgg ggaatgctgg tgcagtggtg tcacggggac aaggtacgaa tcacccgggg
       61 gggatggcaa accattttcc ggtcccgtgc gaaaggcctg cgcgggaata tcgggtaaaa
      121 tcgcccggaa tcggcccgag gatgttcacc aaaaaaaaga accggtagag ccggcacatt
      181 ttgtgagtat tcttgcctgt acggagtgta ggtgttgcac gatggatatg gaaggaaagg
      241 cctgttgaat ggttcgagca ttgttgacgg cttgcgcctg ccttgtactg gcggccgcct
      301 gggcccctgc cgcttcccgc cccgaccccg gggagggtga ccgccttgcg ggggccggca
      361 agcatgcgga tgccgtccgc gaatataaaa aatatgtaca aactgtcggt tactcgcggg
      421 agtcgggccg ggttctcctg gcccttgccc gatcggcgca tgcctcgggt gatcttggtt
      481 cgtttgtctg ggcacgcggg ctttttactc gctggtttcc agtcgagatg ggaccggtca
      541 gggagttcga cgagtatgag gcatcactca ggatgcgggc cggggactgg gccggggcca
      601 tggcccttgt cgaggggctt ctcaagaagt acagctatcc gcccgaggtc ggcgaccgct
      661 ggaggggact cctgatcgcc gggtatgacc gcctggcacg accggacagg gtcttcgaga
      721 tcaggctcga gctggtccgc tccgggagac ggacggcgca ggttgccgat gccctctatc
      781 gtgtttccga gtgggctctc aaaaaggaca agcgggaact ggccctgtac tacgtcaatc
      841 gcctcctctc cgagcatccc ggctggccgc atataagaca tgctgagggc ttgaagagag
      901 ccatcacatg gaagacgatc accgtcgagg atggcctcgg tgacaactcg gtaagtgcca
      961 tccgcttcga cggggatgat atctgggtcg gaacatggct gggtggtgtc acccggtttt
     1021 cacggagcca gggacgcttg caaaaataca cggccaaaaa ttccgggttg gtctcggatc
     1081 tgatccggga tctctgcatc gatcagaccc gggtctggac ggccacgttc gaggggctgg
     1141 cctggttcga caagaggacg gatgcctggc agacggtttt gaccgtggcc gggctggccg
     1201 gtcaacgcat caagtgtgtc accgtttcgg acaaccggct ctgggtcgga accatcggca
     1261 acggtgtttc gatgctggat ttcaggaacc agaagtggag aacctggcgc cgggcggacg
     1321 gactcccgga cgagaacatc gtcacgatca cccatacacc gggttccgtc tgggcgggat
     1381 ccctggccgg cgggattgcg cgctacgatc tcgtggctga aaagtggaag acctgggcgg
     1441 caccgatggg ggctccgctc cagaatatca aggcgacagc ctttgacggg cagcgtctct
     1501 ggatcgctac ccatggggcc gggttgtact cctgcgacga gaacggtacc ggctgggagc
     1561 agtacacgag cgggaattcc ggtttgcccg tcgattttgt gcatgccgtg tccgtcggtc
     1621 ctgacggccg gatctgggcc ggaaccctcg aaggaggggc cgcggtgttc gagcgccgca
     1681 ccggcagatg gaaggtgtac accatccacg atgggctggc cgggaatgac gtgattacga
     1741 ttgcctttga gggacgctat atctggttcg gaaccttgaa tggcgggatc tcggttctcc
     1801 tcggtgacgg ctgatgaccg cggcttcccc tcgcggtata ttcaacagga acgattgtgg
     1861 aggaagacat gagcgagcaa gagacacagc cccggaccgg tgacgaggga cgacaggatc
     1921 ggcccctcgt cctcctggcg gtcgcattct ttctctcctt tcttgccggg atgtttctgg
     1981 ttctgcgggg agaggggatg accggttcgg ccctgcccgg gagcggcccg gagcagcccg
     2041 ggatcgccgt gatcgatatc gagggcgtga tccagtacgc atcccagcaa gggttcatga
     2101 atcccggtgg ggccggcgct cccgtagttg ttgagactgt ccgtcggatg ggcgagaatc
     2161 ctctggttcg cggtttggtg gtccggatca actcaccggg cgggagtatc ggtgcgacac
     2221 aggagatcta ccatgccctg cgtcgttttc gtgagtccgg
//
LOCUS       DAOSHE010000372         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_47198_length_2259_cov_4.856624, whole genome shotgun sequence.
ACCESSION   DAOSHE010000372 DAOSHE010000000
VERSION     DAOSHE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47198_length_2259_cov_4.856624"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLD82_09000"
     CDS             <1..206
                     /locus_tag="PLD82_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65557.1"
                     /translation="AFAAYVLVIHLIGDTLSPAAVGWVSDLASLKTGLEFVTIAITAS
                     GLAFVVVARLVQRGRTGLVRDPG"
     gene            complement(190..639)
                     /locus_tag="PLD82_09005"
     CDS             complement(190..639)
                     /locus_tag="PLD82_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG65558.1"
                     /translation="MDPNDDFRELLELFNAHGIEYAVVGALALAWYGHPRYTGDIDLF
                     VKPDPDNAQKIIAALDQFGFGGLGITIEDFTTPDRVIQLGVPPGRVDLLTSLSGITWD
                     QVSSGSVNGEYAGVPVRYIGKADYIRNKQTVGRHRDLADIEAISQDL"
     gene            865..1095
                     /gene="vapB"
                     /locus_tag="PLD82_09010"
     CDS             865..1095
                     /gene="vapB"
                     /locus_tag="PLD82_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOG65559.1"
                     /translation="MHTVKVFRSGNSQAIRIPREYAVDDSEMYINKIGKVIVMVPKHD
                     PWSAFRESLDSFSPDVFEHGREQPGLQVRESL"
     gene            1092..1505
                     /locus_tag="PLD82_09015"
     CDS             1092..1505
                     /locus_tag="PLD82_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG65560.1"
                     /translation="MKYLLDTNICIFLVKKKFPLMERRVQQEEPFSLAVSAISVAELE
                     FGVAKSLYPEKNRLVLLQFLSLFEILPFSVDDCRAYGEVRAFLQARGILIGPYDLQIA
                     AQCLTRNLCLVTNNCKEFVRVPGLRFEDWTGNLPS"
     gene            1558..2223
                     /locus_tag="PLD82_09020"
     CDS             1558..2223
                     /locus_tag="PLD82_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG65561.1"
                     /translation="MTDTPEKTHKPGKRMQTIGEEIANSITHGIGLAGSLVALAFFIG
                     HLAAGASAREVVSLVMYGLALVFMYQASTLYHAFTGKRIKAIFHRLDHIAIYWLIAGT
                     YAPFSLLFLPDHVGIPLFVAVTSMAVLGTVFKIFFMGKLRILSVVIYLAMGWAVLFAL
                     GPMLEDFPRGLLVWLGIGGLFYTLGVVFYAWRGLPYHHPIWHLFVLGGSAAHLIGIVR
                     YIR"
BASE COUNT          435 a          621 c          628 g          575 t
ORIGIN      
        1 ccgcctttgc cgcctacgtc cttgtgatcc atctgatcgg ggacaccctc agcccggccg
       61 ctgtcggctg ggtctccgat ctcgccagtc ttaaaacagg acttgaattt gtcaccatcg
      121 ccatcaccgc cagcgggctg gcgtttgtgg tcgtggcacg ccttgtccag cgcggaagga
      181 ccgggctcgt cagagatcct ggctgatggc ctcgatatcg gccaggtccc tgtgtcggcc
      241 aacggtttgt ttgttgcgta tataatccgc tttcccgata tatcgcacag gcactccggc
      301 atattcacca ttgaccgatc cgctggatac ctggtcccag gtgataccgc ttaatgaagt
      361 caataggtca acccggccgg gtggaacacc gagctggatg acacgatcgg gtgttgtgaa
      421 atcttcaatg gttattccca gtccgccgaa tccgaattga tcaagcgctg caatgatttt
      481 ttgggcattg tccggatctg gtttaacaaa gaggtcgata tcccctgtgt accgaggatg
      541 tccataccac gccagagcca gtgcccccac gactgcgtat tcaatcccgt gggcgttgaa
      601 taactcgagc aactctctga agtcgtcgtt cgggtccatg gcgcatcctc cagagctgat
      661 tgaccgcctc gatgcgttcg gccggaggtc gggagagcca ccagttacgg tctcgtgccg
      721 cttgatcgct atccaatcga tctctgtgta ccactctctt gatcatacgc acagtatacc
      781 cgttccgccg tcggtctgca agcgcctgtc cgggccttgt tttttgtatg cacacgggta
      841 tatactcaat ggaggaggac tcgcatgcat acagtaaagg tatttcgcag cggcaacagc
      901 caggccatcc ggattcccag ggagtatgcc gttgacgaca gcgaaatgta tatcaacaag
      961 atcggcaagg tcatagtgat ggttcccaag cacgatccgt ggtccgcgtt tcgggagagt
     1021 ctggacagtt tctctcctga tgtttttgag catggcaggg agcagccggg gctacaggtc
     1081 agggaatcct tgtgaagtat ctgcttgata ccaatatctg catctttctt gttaagaaga
     1141 aatttcccct gatggagcgg cgtgtgcaac aggaagagcc cttttcactg gctgtttccg
     1201 ctatctctgt tgccgagctg gagttcggag ttgcaaaaag tctgtatccg gaaaaaaacc
     1261 gacttgtcct cctgcagttt ttgtccctgt ttgaaattct tcccttttca gtcgatgatt
     1321 gccgtgcgta cggagaggtt cgagccttcc ttcaggcgag ggggatcctg attggcccgt
     1381 acgatctgca gattgcagca caatgtctga cacgcaatct ttgtcttgtg acgaataatt
     1441 gcaaggaatt tgtccgtgtc cctggcttgc ggtttgaaga ctggaccggg aatctgccat
     1501 cctgatcgcg ttccatgcta tgttttatgg acgggatgtt cccgatagga ggaatgcatg
     1561 acagacacac cggaaaagac gcacaaaccc gggaagcgga tgcagaccat cggggaagag
     1621 atcgccaaca gcatcacgca cggtatcggt ctggcgggga gcctggttgc cctggcgttt
     1681 tttatcggcc atcttgcggc cggggccagt gcccgcgagg tcgtcagcct tgtcatgtac
     1741 gggctggcac tggtcttcat gtaccaggcc tcgaccctct atcacgcttt cacggggaag
     1801 cgcatcaagg ccatcttcca ccggcttgat catatcgcca tttactggct gatcgccggg
     1861 acctacgcac ccttttcgtt gctcttttta cccgatcatg tcggaatccc gctcttcgtc
     1921 gccgtcacat ccatggcggt attgggaacg gtcttcaaaa tatttttcat ggggaagctg
     1981 cgcatcctct cggtggtgat ctatctggcc atggggtggg ccgtcctctt tgccctcggg
     2041 ccgatgctcg aggactttcc gcgcggactt ttggtctggc tggggattgg cggactcttc
     2101 tatactcttg gcgtggtctt ttacgcctgg cgcggcctgc cgtaccacca tccgatctgg
     2161 catctctttg tcctgggcgg gagtgccgca catctgatcg gcatcgtgcg ctacatccgc
     2221 tgaggggaaa gagatgccag atcggatggt ggtacggca
//
LOCUS       DAOSHE010000373         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_47236_length_2258_cov_3.252837, whole genome shotgun sequence.
ACCESSION   DAOSHE010000373 DAOSHE010000000
VERSION     DAOSHE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47236_length_2258_cov_3.252837"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLD82_09025"
     CDS             complement(<1..1244)
                     /locus_tag="PLD82_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HOG65562.1"
                     /translation="MRTNTNTQPDRPAGTLTHEGARVLPASAIERLRRLTMTCLLWED
                     GFYVDGHTSAQAIKKLVSYLVRHGRAEEVYRVAHEAKHRSQLRHVPLYLARLLAKKGA
                     ITGDQLAALINRVDDIPEFLALYRGTRAPGREPLSRQVKKGLRKAFLSFSPWQLARYR
                     KNDAVFSLRDALLLLHVRPTPELAETFARLAAGTLAASGSWEKELSQGQNKAEVFTRL
                     IQEKKLGALATLRNLRGMQAAGVDMSLVHDWLTRMPVERLLPFQFVKALEYAPELMEG
                     LNTAMLRCLASSSTRLEGTTLLLIDTSGSMESTLSKNSELTRCDAAKGLAILMAEVCP
                     MVHIFEFNHECRSVEQGDAHGLALLSQLSAPDGGTDIDRAVQTALEAHPDAGRLVILT
                     DEQSTSDITHPDLPQRTYIVNVA"
     gene            1954..>2258
                     /locus_tag="PLD82_09030"
     CDS             1954..>2258
                     /locus_tag="PLD82_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG65563.1"
                     /translation="MVQEIERILDGLDDKDRKIILCARSEFAKYGFYNANMDRIALSA
                     GLGKGTLYRRFISKQILFFVTIQADQRELVKRLEQLDTDLPLRQQIETHLAAAVEYIV
                     "
BASE COUNT          433 a          595 c          743 g          487 t
ORIGIN      
        1 gcgacattga cgatgtaggt tcgctgcgga agatccggat gcgtgatgtc gcttgtggat
       61 tgctcgtccg tcaggatgac cagtcgtccg gcatccggat gggcctcaag ggctgtctgt
      121 acggcccggt cgatatctgt tcccccgtcg ggggccgaga gctgggagag aagggccagt
      181 ccgtgcgcat ccccctgctc gactgaccgg cactcgtggt tgaattcgaa gatgtgcacc
      241 atggggcaga cctcggccat gaggatcgcc agccctttgg cggcgtcgca gcgggtgagc
      301 tctgaatttt tgctgagcgt gctttccatt gagccgctcg tatcaatgag gagcagggtt
      361 gttccctcga gtcgtgtgct ggatgaggcc aggcagcgta gcatggctgt attgagtccc
      421 tccataagct cgggcgcata ttccagagcc ttgacaaact ggaatggcag gaggcgttcg
      481 accggcatgc gggtcagcca gtcatggacg agtgacatgt ccaccccggc ggcctgcatc
      541 ccgcgcaggt tgcgcagtgt cgccagggcc cccagttttt tttcctggat cagccgggta
      601 aagacttcgg ccttgttttg cccttgtgag agctcttttt cccaactccc ggatgcggcc
      661 agcgttcctg ctgccagtct tgcaaaggtc tccgccagct cgggtgtggg ccggacatgg
      721 agcaggagga gggcatcccg taggctgaag acggcgtcgt ttttgcggta gcgggccaat
      781 tgccagggag agaaggagag gaaggccttg cgcagcccct ttttgacctg gcgggagagc
      841 ggctcgcgtc cgggagcccg ggtcccccgg tacagggcca aaaattcggg gatatcgtcg
      901 acgcggttga tcagggccgc caactggtcg cccgtgatcg cgcccttttt agccaggaga
      961 cgggcgagat acagggggac gtgccgcagt tgcgagcgat gcttggcttc gtgggctacg
     1021 cggtagacct cctcagcccg gccgtgtctg accagatagg acacgagttt tttgatggct
     1081 tgggcgcttg tgtgtccgtc gacgtaaaat ccgtcttccc acaacaggca ggtcatggtc
     1141 aggcggcgca gccgttcgat ggccgaagcg ggtaggacac gggcaccttc gtgggtcagg
     1201 gttccggcag ggcgatcagg ttgtgtgttg gtattggtgc gcatgcaatt ctcctctgag
     1261 gcatcaaaca gtgatacggt cggcaggagt tttttatgca acaaaatttt ttgctctctg
     1321 tgcataaaaa tcctttcgag cgcgtatctc agggtatggg tggtgcaggg tacagcttca
     1381 taatcaaggt gaggcagtgc ccgcatggcg gagaccgggt cggtgccgtt ggccgaccgg
     1441 gttcatgcgg gagtgtgtgc tgtccccgat tttcccccat acggtgcccc tttcccgtcc
     1501 gttctgcgct gtttgcaggc gggcggtttt tttttgatga acagcctcag gccagcgcgg
     1561 actcggcggg agcgtccagg ggatgcgggc ctgcactcct cctgacgaag agtccgtcac
     1621 cggtcgggtt gcgatcgggc caagggcagc cattgcagga acggggtgat ctgaaaagcg
     1681 tgtgcatccg tcggaccagt ggcaggaatg gcgggtgccg gggacgccga aaccggaaaa
     1741 aatcgagcgc gagcgtgttc tccaaaacgc tgttgcacca tggtgttggc gacgcatgag
     1801 aagcgtcatt aattatggta ggaacgcaaa aagtgagcga aaatcggccg gatgaggtcg
     1861 tcggcggaga attcccgcaa aaagacacca aaaaagtgga ctgaccagtc cgtccgcagt
     1921 accttgaaag gacatacgta caaggagcgg accgtggttc aggagattga gcggattttg
     1981 gatggattgg atgacaagga ccgcaagatc atcctctgtg cccgttccga atttgccaaa
     2041 tacgggtttt acaatgccaa catggaccgg attgccctgt cggccggtct tgggaagggc
     2101 acactctacc ggcgcttcat cagcaagcag atcctctttt ttgtgaccat ccaggctgac
     2161 cagcgtgagc tggtcaagcg gctggagcag cttgataccg atctgccgct cagacagcag
     2221 atcgaaaccc atctggctgc tgcggtggag tacatagt
//
LOCUS       DAOSHE010000374         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_47724_length_2242_cov_3.689529, whole genome shotgun sequence.
ACCESSION   DAOSHE010000374 DAOSHE010000000
VERSION     DAOSHE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47724_length_2242_cov_3.689529"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLD82_09035"
     CDS             complement(<1..397)
                     /locus_tag="PLD82_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65564.1"
                     /translation="MEHIANAKKRAQGLHFATNCYPDPKKTSYWIATGLWAEHSASGL
                     LLWRHDQKCWHLYYAGPATSTQKMVQSAQFDTMSQPVVVDIVTKNPDEPILHDLYTTG
                     FRKHTTLTRMIQTPPGNADPAHPPGIRTTT"
     gene            complement(427..1947)
                     /locus_tag="PLD82_09040"
     CDS             complement(427..1947)
                     /locus_tag="PLD82_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOG65565.1"
                     /translation="MHTNVFEYLEQTISRLPEEVAVRDSGRSLTFSDLHTAALSGATR
                     ILEKNPEKNQPIAIFLPKSSEALIAVCSILASGNCYAPLDIKSPESRLSAILDSLGNP
                     LVVTKTEFQETLVRLGVPAPQIILIDSLPETTDPAALARHRSSVIDTDPAYIIFTSGS
                     TGTPKGVAISHRSIIDYIEWARSVYPVNNSDRIASQAPLHFDNSTLDIYLCLSCGAAF
                     VIVPDQLFSFPAKLVQFLRDESISMIFWVPSILVNAANLKALDGVSLPALDKVLFAGE
                     VMPNKHLNYWRKQLPHALFSNLYGPTEITVDCTYLLVDRDYSDDEPLPIGIPCRNSDI
                     IILNEQNQRVTTSGQIGELCVRGSSLALGYWNDPEKTAQAFVQNPLQTHYPERIYRTG
                     DLVSLNNQGEIMFIGRKDSQIKHMGYRIELGEIETALLGCPGIENGCVLYNTAKKEIT
                     ALYTAAEDIPVATLRSELGKSLPQYMLPRTAIRLDIMPLSANGKIDRLALTKAYLT"
     gene            complement(2007..>2242)
                     /locus_tag="PLD82_09045"
     CDS             complement(2007..>2242)
                     /locus_tag="PLD82_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65566.1"
                     /translation="GLYVIISHFLGNTVPPGWTTIVASIFFVGGIILAVIGITGIYLG
                     QVFDEVKGRPLYVISRRTRQPSRPPNKPSSSRR"
BASE COUNT          489 a          485 c          686 g          582 t
ORIGIN      
        1 gggtggtggt gcgaataccg ggtgggtgtg cagggtctgc attgccgggg ggtgtttgta
       61 tcattcgggt gagggttgta tgcttgcgaa aaccggtcgt gtacagatca tgaagtatgg
      121 gttcgtccgg gttttttgtg acaatatcga cgacaactgg ctgtgacatt gtatcgaatt
      181 gcgccgattg caccatcttc tgggtggagg ttgctgggcc agcatagtat aaatgccagc
      241 atttttgatc atgccgccat aacagcaaac cggaggcgct gtgttcggcc cataatccgg
      301 tggctatcca gtagctggtt tttttcgggt ccggatagca attcgtggca aaatgcaggc
      361 cttgtgccct tttttttgcg ttggcgatgt gttccatcag ttccggatag gatgtgattt
      421 gagatatcat gtcaggtatg ctttagtcag tgccagccgg tcaatttttc cgttggccga
      481 gagaggcatg atgtcaagtc gaatggcggt acgaggaagc atgtactggg ggaggctttt
      541 tcccaactcg ctgcgcaggg tggctaccgg gatgtcctca gccgctgtgt acaaggctgt
      601 gatttctttc tttgctgtat tgtatagaac acagccgttt tctattcccg gacagcctaa
      661 aagtgcggtt tcgatttcac caagctctat gcgatatccc atgtgcttga tctggctgtc
      721 cttgcgtccg ataaacatga tttcaccctg gttgttcagg ctgacgagat ctcccgtgcg
      781 atagatacgt tcgggataat gtgtttgcag ggggttttgt acaaaggcct gtgcggtttt
      841 ttcggggtca ttccagtacc cgagtgcgag ggaactgccc cggacacaga gttctccgat
      901 ttgcccgctt gttgtgacac gctgattctg ctcgttgagg atgatgatgt ccgagttgcg
      961 gcatggtatt ccgatcggca gaggttcatc atcagaataa tccctgtcga ccaacagata
     1021 ggtgcagtcg actgtaatct ctgtcgggcc gtagaggttt gaaaaaaggg cgtgaggaag
     1081 ctgttttctc cagtaattga gatgcttgtt gggcatcact tcaccggcaa agagcacctt
     1141 gtcaagcgca ggcaggctga ctccgtcgag ggccttgagg ttggctgcgt tgacaaggat
     1201 cgaaggaacc caaaaaatca tgctgatgct ttcatccctg aggaattgca ccagttttgc
     1261 cggaaaggag aacaattggt cagggacgat gacaaaggcc gccccacatg aaaggcacag
     1321 gtagatgtcc agggttgaat tgtcaaaatg aagcggagcc tggcttgcga tccggtcgct
     1381 gttgttgacc gggtatactg accgcgccca ttcgatatag tcgatgatgc tccggtggct
     1441 gatggcaaca cccttcgggg ttcccgttga ccctgaagtg aagatgatat aggcagggtc
     1501 ggtgtcgatg acggaagagc ggtgacgtgc aagtgctgca ggatcagtgg tttcgggcag
     1561 cgagtcgatc aggatgattt gtggggcagg gacaccaagt ctgacaaggg tttcttgaaa
     1621 ttccgttttg gtgacgacaa gcgggttgcc gaggctatca aggattgcgg atagccggct
     1681 ctcgggtgac ttgatatcca gaggcgcgta gcagttgccg ctggccagga tggagcagac
     1741 tgcgatcaat gcctcggagg atttgggaag aaaaatggct atgggctggt ttttttccgg
     1801 gtttttttca agaattcggg ttgcaccgga cagggccgcc gtgtggagat cggaaaacgt
     1861 cagactgcgg ccgctatcgc ggacagcaac ctcttcaggc agtctggaaa tggtttgttc
     1921 gaggtattca aagacattgg tatgcattgc tgtaatccgc ccattaatca ttgatgcagt
     1981 gataaatata ccttttttgg cggattttag cgcctggatg aagacggttt gtttggtggc
     2041 cgggagggct gacgggtgcg tcgggaaatt acatagagtg gcctgccctt gacttcgtcg
     2101 aaaacctgcc caaggtagat tccggtgatg ccaatgacgg cgagaatgat gccacccaca
     2161 aaaaaaatcg aggcaacaat cgtggtccag ccgggcggga ctgtgttccc cagaaaatgc
     2221 gagatgatga catagagccc at
//
LOCUS       DAOSHE010000375         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_48088_length_2231_cov_3.306985, whole genome shotgun sequence.
ACCESSION   DAOSHE010000375 DAOSHE010000000
VERSION     DAOSHE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48088_length_2231_cov_3.306985"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLD82_09050"
     CDS             complement(<1..688)
                     /locus_tag="PLD82_09050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG65567.1"
                     /translation="MYNYENQDYLQHLTEKSELWYQDVLAALLSVQPKEVLELGCGDG
                     GFSRLMAEQKIRVLALDHSKTFLEYARRRTKSRLVQFEQANLDSAVGLHIAGRRFQAV
                     VSVDVIEHLRRKERLLKDVAGVLEEDGYLVLQVPNLYCNMISQNYVHSPINLLRKFRR
                     GVRGAWRQFRHKPPEDICRVEYGLDYRVADHDAVWLSSPFWYLDFFRRHNWELVSCTT
                     WSFDTKKVWLK"
     gene            931..1821
                     /locus_tag="PLD82_09055"
     CDS             931..1821
                     /locus_tag="PLD82_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG65568.1"
                     /translation="MNRWLRGIWILGILCSLGRPGYGVYISEVMAHPAGGGGAADEYI
                     EICNPGRETIDLGTCLLATPGGKRQALIPWNGPFSGIPAGILLAPGGIALITAPGYTG
                     RYRDWLSATPHQAGRIHLTIDGSRLAVHGLPNDGGAILLEDGNGKLLDVLVWFEDAGE
                     GTSWERGPGPSAPLVRRYHGGTPGSAAVPLFELTKKPERFVLEQPVSTRDRPGTAILT
                     LKTGESARICLFTLGGRRIAEVSGQLGGPGVWRIPVPREQEDGRLLPVGRFCLTAEIS
                     LENGESIHVTRLLRIAPHHR"
     gene            1781..>2231
                     /locus_tag="PLD82_09060"
     CDS             1781..>2231
                     /locus_tag="PLD82_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65569.1"
                     /translation="MSHVCSGLRPIIGKLLLCLSFGVSLHGESAGTLVDHAGSLARSW
                     TGIASPAGALSVLHNPALIPAGFEYAYGFSGGLVPDVEINTAVTGLVLDFPGNWGIGL
                     GWSLSSDGLWDEQCFSLGTGVSLFPGFSAGLQARLIRLSIDGYNAWTT"
BASE COUNT          448 a          693 c          650 g          440 t
ORIGIN      
        1 gcttgagcca gacttttttt gtgtcaaatg accaggttgt acatgaaacc agctcccagt
       61 tatgcctgcg aaaaaagtcc agataccaga agggagagga aagccaaaca gcatcgtggt
      121 cggccacccg gtaatcgaga ccgtactcga cccggcagat gtcctcgggc ggcttgtgcc
      181 tgaactggcg ccaggccccg cgtacgccgc gcctgaactt gcgcaggagg ttgatcggag
      241 aatggacata gttttggctg atcatgttgc aatagagatt gggaacctgg aggacgagat
      301 acccgtcttc ctcgagcaca ccggccacat ccttgagaag ccgttccttg cgcctgagat
      361 gctcgatcac gtccaccgag acaacagcct gaaaccggcg accggcaata tggagaccaa
      421 ccgcactgtc aagattggcc tgttcgaact ggaccagccg gctcttggtc cggcgccggg
      481 catactcgag aaacgttttt gaatgatcca gggccaaaac ccggattttc tgctcggcca
      541 tcagtcgcga gaacccgccg tcaccgcatc ccagctcaag gacctccttc ggttgcaccg
      601 agaggagggc tgcgaggaca tcctgatacc agagctccga cttttcagtc agatgctgca
      661 ggtaatcctg gttttcgtag ttgtacatgg ggcacccgag aacaatgtat ccgaaattta
      721 cccggaacgc cagccttttt caagtctcct gttccgaccc tcgggcacac ccatgccggg
      781 aaaaatccgc tccagacaga gcgcacaatc cctatacctc cccgtcatgg cgtgactcgg
      841 tacccgccgg gcagattttt tgcattttca tggcaccgcc aatctctccc ggacctctcc
      901 tctcatggaa agatgaaggg aggaacaggc atgaaccgat ggctccgcgg tatctggatc
      961 ctgggaatac tctgcagcct cgggagaccg ggatacggag tgtacatcag tgaggtcatg
     1021 gcccacccgg ccggcggcgg tggtgccgca gacgaatata tcgagatctg caacccgggg
     1081 agagagacga tcgatctcgg aacctgcctg cttgcgacac cgggcggaaa acgtcaggcc
     1141 ctgatcccct ggaacgggcc attctccggg attccggccg ggatcctcct ggctccaggc
     1201 ggaatcgccc tgatcaccgc ccccggctac actggacgct atcgcgactg gttgtccgcc
     1261 acaccgcacc aggcaggccg gatccatctg accatcgatg gatccaggct cgccgtacac
     1321 ggactgccca atgatggtgg cgcaatcctc ctcgaagacg gaaacggaaa actcctcgac
     1381 gtcctggtct ggttcgagga cgcgggagag ggcacatcct gggaacgtgg gcccggacca
     1441 tcggccccct tggtccgccg ctaccatggt gggactccgg gatcggcggc cgtccccctc
     1501 ttcgagctca cgaaaaaacc ggagcgcttt gtactggagc agccggtcag tacgcgggat
     1561 cgtcccggaa ccgccatcct caccctgaaa accggggaga gcgcccggat ctgcctcttc
     1621 acgctgggcg gacggaggat tgcagaggtc tccggtcaac tcggcggacc gggcgtctgg
     1681 cgcatcccgg ttccacggga acaggaggac ggccggcttc tgccggttgg ccgcttctgt
     1741 ctgacagcag agatcagcct ggagaacgga gaaagcatcc atgtcacacg tctgctcagg
     1801 attgcgcccc atcatcggta agctcttgct ctgtctctca ttcggcgttt ccctgcatgg
     1861 agagagtgca ggcactctgg tggaccatgc cggatcactg gcacgttcct ggaccggaat
     1921 tgccagtccc gccggagcac tctccgtcct gcacaacccg gccttgattc cggccggatt
     1981 cgagtatgcc tacggattct cgggagggct cgttccggat gtcgagatca acacggctgt
     2041 cacgggtctt gtccttgatt tccccggaaa ctggggaatc gggctgggct ggtctctctc
     2101 ctcggacgga ctctgggatg aacagtgttt ctcgctgggt accggcgtct cattgttccc
     2161 gggattctcg gcgggtctcc aggcccgtct catacgcctc tcgattgacg ggtacaatgc
     2221 ctggaccacg c
//
LOCUS       DAOSHE010000376         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_48129_length_2230_cov_2.364138, whole genome shotgun sequence.
ACCESSION   DAOSHE010000376 DAOSHE010000000
VERSION     DAOSHE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48129_length_2230_cov_2.364138"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PLD82_09065"
     CDS             <1..1630
                     /locus_tag="PLD82_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65570.1"
                     /translation="LGFLTFREFVRYTDSTIRTVLVAIFKHLRNATLWIALSVTVFAV
                     YTYARSGQFPDFSGALSYIGIFYSSGLAMMPLPLFHTWELVAVIYIGAITLACCTRLF
                     RGTRPAPSGSFDPAILLTLSLLGILLFSYYQGRSHDWNLYKVWWPAFIILTLATDRLW
                     KAATNRILPFHGFQFFRLFSVQRAGTGRLFSLIASLSLLFLLAAALPSSAITGLRMNK
                     FFKGRHSAKNRVMESEFAQSSLFIQSHTKPGEKVLIFSYRDASFHVQSRTSSPVPVPG
                     MTELLLKSELDAILAYIGSGNADRFFVEPALADNALMTRAFTAGYWPLAFSPHNTIAL
                     FAKTNTGSPVGKPAASRLLPAPGVSTIEHFFGHGPFSISRDGVIAGLLGSTPKRFAPL
                     DGFSIECTLTALPDQPSDAFLFGNPPRSGTPGGFLIQKQGTKPGEYALVWGHARSWQP
                     RLLFTIPSGKKAHLVATGSKASGRVEVFVNGMHAGSARYPGMDISPDPILIGRIGNSY
                     TPFAGSLHEIRLFNQVLDANQCRAMAAYALQSPD"
     gene            complement(1633..>2230)
                     /locus_tag="PLD82_09070"
     CDS             complement(1633..>2230)
                     /locus_tag="PLD82_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65571.1"
                     /translation="PLVYIAVMLPAWIAGRSMGDLLTIYVGQAKMFSSLTLHLPNMYE
                     WLPAQPVLSTWGLVATGAFVIGIVWFALSQKKQEGPVLQVQIALLSALAIPFLLPHMH
                     ERYYFIADVLAILYAFLVPGRWYLPVMIGGTSFFAYQPFLFAKQGPVPAPILALVIVA
                     AIGFVARDMIRSYAEKTDRQAIPKPARKFRRRYALRRS"
BASE COUNT          485 a          696 c          566 g          483 t
ORIGIN      
        1 gctgggattt ttgactttcc gcgagttcgt ccgctatacc gactcgacca tccggacagt
       61 cctggtcgcg attttcaagc acctcagaaa cgcaacgctc tggatagcac tctcggtgac
      121 cgtctttgcc gtgtacacat acgcccggag tggccagttc cccgattttt ccggtgccct
      181 gtcctacatc gggatcttct attcgtcggg ccttgccatg atgcccctgc ctctgtttca
      241 tacatgggaa ctggttgccg tcatctatat cggggctatc acccttgcgt gctgcacacg
      301 actctttcgc ggaacccgcc cggcaccctc ggggtccttc gatccagcca tcctgttgac
      361 cctctcactg ctgggaatcc tgctcttttc ctattaccaa ggccggagtc acgactggaa
      421 tctgtataaa gtctggtggc cggcattcat catcctcacc ctggccacag acagactgtg
      481 gaaggcggcc acaaaccgga ttctcccctt ccatggtttc caattcttca ggctcttctc
      541 cgtccagcga gccgggacag gccggctctt ttcactgatc gcctcgctct ctctcctttt
      601 cctgctggca gcagcgctcc cctcttccgc aattaccgga ctcaggatga acaaattttt
      661 caagggaagg cacagtgcaa aaaaccgggt catggaatcc gagtttgcgc aatcatccct
      721 gtttatccaa tcgcatacca agcccggcga gaaggtcctg atcttttcct atcgtgacgc
      781 gtctttccat gtccagtccc ggacatccag tcctgtgccc gtccccggca tgaccgagct
      841 cctgctcaaa tccgagcttg atgcgatcct ggcctatatc ggcagcggga acgctgaccg
      901 cttttttgtc gaaccagccc ttgcggacaa tgcactgatg acacgtgcat tcacggcagg
      961 ctactggcct ctcgcgttct ctccgcacaa caccattgcc ctcttcgcaa agacgaatac
     1021 tggctctccg gtcggaaagc cagccgcatc ccgactgctg cctgcccctg gagtgagcac
     1081 gattgaacac tttttcggcc atgggccatt cagtatcagc agggatggtg tcatcgccgg
     1141 actccttggt tccaccccaa aacggtttgc acccctggac gggttttcca tcgagtgcac
     1201 gctcacggca ttgcccgatc aaccctccga tgccttcctc ttcgggaatc ccccacgctc
     1261 cgggacaccg ggtggattcc tgatacaaaa acaggggaca aagcccgggg aatatgcctt
     1321 ggtctggggc catgccagga gttggcagcc cagactgctg ttcaccatcc cgtccgggaa
     1381 gaaggcccat ctggttgcaa ccggctccaa agcatcgggc agagtcgagg tctttgtcaa
     1441 cggcatgcac gcaggttcgg cgagatatcc gggcatggac atctcgcccg acccgatcct
     1501 gatcggccgg atcggaaaca gctacacacc ctttgccggt tcattgcacg agatccgtct
     1561 cttcaaccag gtgcttgatg caaaccagtg cagggcgatg gcagcctacg ccctgcaatc
     1621 cccggactga catcaactcc ggcgcagcgc atacctgcgc cggaatttcc gggctggctt
     1681 cgggatggcc tgtctgtcgg tcttttccgc atacgaacgg atcatgtctc tcgccacaaa
     1741 tccgatggcg gcaacaatca cgagagccag aatcggcgct ggaacaggac cctgtttggc
     1801 aaataaaaat ggttgatagg caaaaaacga cgtgccacca atcatgaccg ggagatacca
     1861 gcggccggga acaagaaatg cgtacagaat cgccaacaca tccgcgatga aataataacg
     1921 ctcatgcatg tgcggcagga gaaaagggat ggccagcgct gaaagaagcg caatctggac
     1981 ctggagaacc ggaccctcct gttttttttg cgaaagggca aaccatacaa ttccgatgac
     2041 aaaggcccct gtcgcaacca gaccccaggt cgaaagcaca ggctgtgccg gaagccattc
     2101 gtacatatta ggaagatgga gcgtcagact gctgaacatt ttggcctgac cgacatagat
     2161 ggtcaacaaa tcgcccatgg atcggccggc aatccaggct ggcaacatga cagcgatata
     2221 gaccaacggg
//
LOCUS       DAOSHE010000377         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_48302_length_2224_cov_3.771323, whole genome shotgun sequence.
ACCESSION   DAOSHE010000377 DAOSHE010000000
VERSION     DAOSHE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48302_length_2224_cov_3.771323"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2164)
                     /locus_tag="PLD82_09075"
     CDS             complement(<1..2164)
                     /locus_tag="PLD82_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOG65572.1"
                     /translation="MGRAGAFSFERDIVPLLPRVAKPARYTGGEYNSIVTGDADCVIA
                     LAYPDVYEIGMSNLGLRILYEALSRVEGCAVERVYLPWPDMADMLREKGLPLWTLETK
                     KPLSACAVLGITLQYEMTFTNILELLSISGIPLRREDRTTDHPLIVAGGPCAFNPVPL
                     APWIDVFVLGDGDQAAVDLLTTVRDAQRDGASREAMLGRFARFEWAWIPGLKENAKRA
                     IIPDLDEAPFPVRQIVPNVRAIQDRGLIEASRGCTNGCRFCHAGMTYRPVRERSPDQL
                     VETAVSLVNSTGFDEVSLASLSISDYTRLEELIIRLNAALGPRNVSFSLPSLHVDGFT
                     LETATLVGELRKSGLTFAVEAGDPAMRARINKPVDDDHLMSIFRTVWDAGWKQVKLYF
                     MIGFPGAHDEAEVIADFIRRAHAAAPRLAINCNVGIFIPKPHTPLQWARQMSIEDGWT
                     AINQLRERFKKGNVKISWHMPEMSFLEGVFARGGDKAARLLDEAWKRGARFDAWDEHF
                     SFRRWKDAMDALGYTEEEFLAPFASTDDSLPWDGISGVAGKGFLLRELQKYVTGMSQE
                     DARLAGVHTSVAGENLPDLTEDCRQGCRSHCGVCTPETGPRMAPPGSEPLPKSVPDTN
                     TTPAVHPTSLPGDASRERHVYRIRFDKTGLLKFISHLDMQQHLIRIFQRSALPLSVSE
                     GFNPKPRLSLVAPAMLGMATTNDILEIELAARLPEADIV"
BASE COUNT          475 a          654 c          678 g          417 t
ORIGIN      
        1 caacaatatc cgcctcgggc agacgggctg cgagttcgat ctcgaggatg tcattggtcg
       61 ttgccatccc cagcatggcc ggagcgacaa gggaaagccg cggcttggga ttgaagccct
      121 cactgaccga aagcgggaga gcgctgcgct ggaagatgcg gatcaggtgt tgctgcatat
      181 cgaggtgcga gataaattta agaagtccgg tcttgtcaaa acggatgcga tagacatggc
      241 gctcgcgact ggcatcaccc gggaggctcg tcggatgaac agccggggta gtattggtat
      301 ccggcacgct tttgggaaga ggttcgctcc ccggaggagc catcctgggc ccggtctcag
      361 gggtgcagac accgcaatgc gagcggcagc cctgcctgca gtcttcagtc aggtctggca
      421 gattctcgcc ggctacggac gtgtgcaccc cggccagtct ggcgtcctcc tgggacatcc
      481 ctgtaacata tttctgcagt tcgcgcagca aaaacccctt gccggccact ccgctgattc
      541 catcccaggg gagtgaatca tctgtactgg cgaagggcgc aaggaactcc tcttcggtat
      601 acccgagggc atccatggca tccttccagc gccggaagga aaaatgctcg tcccaggcat
      661 cgaagcgggc accgcgcttc caggcttcat cgagcagccg ggcagccttg tcgccacctc
      721 gggcgaagac accttcgaga aaactcatct cgggcatgtg ccaggatatc ttgacattcc
      781 cctttttgaa ccgctcgcgc aactggttga tggccgtcca tccatcctcg atggacatct
      841 ggcgtgccca ttgcagcggc gtgtgcggtt tgggaatgaa gatccccaca ttgcaattga
      901 tggcgagtct gggtgcggcc gcgtgagccc gccggataaa gtcggcaatc acctcagcct
      961 cgtcgtgagc accggggaac ccgatcatga aatagagttt gacctgcttc cagccggcat
     1021 cccagaccgt gcggaagatg ctcatcagat ggtcgtcatc caccggcttg ttgatccggg
     1081 cacgcatcgc cgggtccccg gcctcgacgg caaaagtcaa tccgctcttg cgcaactcgc
     1141 caacgagggt ggccgtctcg agggtgaatc catccacatg cagggagggc agcgaaaacg
     1201 agacattgcg cggccccagg gccgcattga ggcggatgat cagttcttca agccgtgtat
     1261 aatcgctgat ggacagggag gcgagactga cctcgtcaaa gcctgtactg ttgaccagtg
     1321 aaaccgcggt ctcgacaagc tggtccggac tccgctcacg gaccggacga taggtcatgc
     1381 cggcatggca gaaacggcac ccgttggtac atccgcggga agcctcgatc aggccgcgat
     1441 cctggatggc ccggacattg ggaacaatct gccggaccgg aaacggcgcc tcgtccagat
     1501 cgggaatgat ggcgcgtttt gcgttctcct tcaggccggg gatccatgcc cactcgaacc
     1561 gggcaaaccg cccgagcatg gcttcacggc ttgccccatc ccgttgggca tcccggacgg
     1621 tcgtgagcaa atcaaccgcg gcctggtctc catcgcccag gacaaagaca tcgatccagg
     1681 gagcgagtgg cactggattg aaagcgcagg gaccaccggc aacaatcagg ggatgatcgg
     1741 tggtcctgtc ttcacgacgg agcggaattc cggaaatcga gagaagttcg aggatatttg
     1801 tgaacgtcat ttcgtactga agggttatcc cgaggactgc gcaggccgaa agaggttttt
     1861 tggtctcgag tgtccagagc ggcaggccct tttccctcag catgtctgcc atgtcaggcc
     1921 aggggagata gacccgttcg acggcgcaac cctcaacccg cgagagagcc tcgtagagga
     1981 tgcgcaaccc aagattggac atcccgattt cgtagacatc gggataggcc agggcgatga
     2041 cacaatcggc atccccggtg acgatgctgt tgtactcacc accggtatag cgcgcgggtt
     2101 tggcaacccg tggcaggagc ggaacgatat cccgctcgaa cgaaaaggct cctgcccgac
     2161 ccatgcccgt caatggacat acctgcgcat ctcgatgttg aagagaagtc cgacggcaat
     2221 cagg
//
LOCUS       DAOSHE010000378         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_48506_length_2218_cov_2.600092, whole genome shotgun sequence.
ACCESSION   DAOSHE010000378 DAOSHE010000000
VERSION     DAOSHE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48506_length_2218_cov_2.600092"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLD82_09080"
     CDS             <1..470
                     /locus_tag="PLD82_09080"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG65573.1"
                     /translation="LEGARPEDVRAADSAFRQAANAYKEAMVYHDELKLTSPIDGEVM
                     KRIVKAGEIAAAGYPVLTLVDLSDVWVVLQLREDRLPTVSMGKILKGKIPALGNKSFD
                     FRVTYIAPMADFATWRPTNQKGDFDLKTFEIHLRPATNIAGLRGGMSVNVTVE"
     gene            483..1658
                     /locus_tag="PLD82_09085"
     CDS             483..1658
                     /locus_tag="PLD82_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65574.1"
                     /translation="METRGTGVFAIAMRELGRITASRSLILLAVVIPLILFILFSWIY
                     DRGIVRELPVAVCDLDRGALSRTVITMVEASSSMRIAWRVTDPEELRDLMLQGKAEGA
                     IIIPRDFERDIKHGRRSAVIVYKNSANLIVGNLIYRDAQTIIRTVSAGVVLKKLQSSG
                     MDKTAAMGVVNPIKLEVQSLYNATYNYQQYLVPGLIAAMLQMLVMMIAAIAVNSEHAT
                     GGMKELAKLSGGRAWRILVGKALGYLPVHGATVLAVLGIVFPLFGIEVSGFVPFIILY
                     FMYFILASFLLGFAVSCFFKDSMMATVIIVFINTPAFIFSGFTYPLWAMPEIHTLYAQ
                     AMPFTHFLSGFLKSYQMDAPVSTAMGEFVSLSIFVGVGLVLSLVGVWIHVRRVRGGV"
     gene            1658..>2218
                     /locus_tag="PLD82_09090"
     CDS             1658..>2218
                     /locus_tag="PLD82_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65575.1"
                     /translation="MRAWLSVFRTVFLRELSIIRSDRNILMIVMIAPLFYALFYSTIY
                     MNKSERDVPIAVVNLDHSDASRALERRLDAHQLVRVALRLPDLGEARALIEKMEIYGV
                     VYIPKDYGAHLSENRSPVIKVWLNTTRFLVSNDINKAINEVVLEAGSKHRMAFFNLKG
                     INGDAASVMADPVMPDVRSVFNVTDSY"
BASE COUNT          439 a          574 c          651 g          554 t
ORIGIN      
        1 ccctcgaagg ggccagacca gaggatgtgc gtgccgcgga ttccgccttc cggcaggcgg
       61 ccaatgccta caaggaagcc atggtctacc atgacgaatt gaaactgacg tctccgattg
      121 atggagaagt gatgaagcgg attgtcaagg ctggcgagat tgcggcagcc ggatacccgg
      181 ttttgacgct ggttgatttg tcggatgtct gggttgtcct ccagttgagg gaggacagat
      241 tgcccaccgt cagtatgggg aagattctca aaggaaagat tccggctctt ggcaacaaat
      301 cgttcgactt ccgggtgacc tatattgcgc cgatggcaga ttttgcgacc tggcgaccga
      361 caaaccagaa gggtgatttt gatctcaaga ccttcgagat ccatctgcgc cctgccacga
      421 atattgccgg attgcgcggt ggcatgtcag tcaatgttac ggtcgagtag gcgatgcccg
      481 gcatggagac gagaggaacc ggcgtatttg cgatagccat gcgagaactc ggcaggataa
      541 ccgcgagcag gtcgctgatc ctgcttgcgg tggtcatccc gctgattctc ttcattcttt
      601 tttcctggat ctacgaccgg gggatcgtcc gggagctccc ggttgccgtc tgtgatctgg
      661 accggggtgc actctcccgg accgtgatca ccatggtcga ggcctcgtct tccatgagga
      721 ttgcctggcg ggtcaccgat cccgaagagt tgcgggacct catgcttcaa gggaaggccg
      781 aaggggccat catcattccg agggattttg aacgggacat caaacatggg cgacgctcgg
      841 cggtcatcgt ctacaagaac agcgccaatc tgattgtggg aaacctgatt tatcgtgatg
      901 cacagaccat catccggacg gtctctgccg gtgtggtcct caaaaaactc cagagttccg
      961 ggatggacaa gacggcagcc atgggggtag tcaacccgat caagctggaa gtgcagtcac
     1021 tctacaacgc gacgtacaat taccagcagt atctggttcc cgggttgatt gcggccatgc
     1081 tccagatgct tgtcatgatg attgcggcga tcgcagtcaa ttcggaacat gccacagggg
     1141 gaatgaaaga gcttgccaag ctctcgggag ggcgtgcctg gcggattctt gtcggaaagg
     1201 cgctcgggta tcttccggtg catggtgcga ccgttctcgc ggtcctcgga attgtttttc
     1261 cgctcttcgg gatcgaggtc agcgggtttg tgccgttcat cattctgtat ttcatgtatt
     1321 tcattcttgc gtcatttttg ctcggctttg cggtttcctg cttcttcaag gacagcatga
     1381 tggcgactgt catcatcgtt ttcatcaaca ctccggcgtt catcttcagc gggtttacct
     1441 atcccctctg ggccatgccg gagatacata ccctgtatgc gcaggcgatg ccttttaccc
     1501 attttctttc gggttttctc aagtcgtacc agatggatgc gccagtatcg acggcgatgg
     1561 gtgagtttgt cagtttgtcg atctttgtcg gggtgggcct ggttctttcg ctggttggag
     1621 tctggatcca tgtccgtcgt gtgcggggag gtgtctgatg cgggcctggc tctcggtctt
     1681 caggacggtt ttcttgcggg aactctccat catcaggagc gaccggaata tcctcatgat
     1741 tgtgatgatc gcgccgctct tttatgccct gttctacagc acgatttaca tgaacaaatc
     1801 cgaacgggat gttccgatcg ccgtggtcaa cctcgatcac tcggatgcat cccgggcgct
     1861 tgaacggagg ctggatgcac accagcttgt ccgtgttgcc ctgcgactgc ctgaccttgg
     1921 agaagcccgg gccctgatcg agaaaatgga gatctacggg gtggtctata ttccgaagga
     1981 ctatggggcc cacctctcgg aaaatcggtc accggtcatc aaggtctggt tgaacacaac
     2041 gcggtttctt gtttcaaatg acatcaacaa agcgatcaac gaggttgttc ttgaggcggg
     2101 ctcaaagcac cgcatggctt ttttcaatct caaagggatc aacggggatg ccgcctcggt
     2161 catggctgac ccggtcatgc cggatgtccg gtccgtcttc aatgtcacgg acagttac
//
LOCUS       DAOSHE010000379         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_48770_length_2210_cov_4.232019, whole genome shotgun sequence.
ACCESSION   DAOSHE010000379 DAOSHE010000000
VERSION     DAOSHE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48770_length_2210_cov_4.232019"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2210)
                     /gene="ileS"
                     /locus_tag="PLD82_09095"
     CDS             complement(<1..>2210)
                     /gene="ileS"
                     /locus_tag="PLD82_09095"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG65576.1"
                     /translation="LPVENEIEKKLDLKTKRDIENFGIDRFNEECRSIVLRYVGEWEK
                     TVERMGRWVDFKNAYITMDLSYMESIMWVFRQLWDKGLIYRSFKILPYCPRCATPLSN
                     FETNQGYKEVADPAITVRFALEGRERTYILAWTTTPWTLPSNMALAVGAEIRYVEVVD
                     GEDRFILAKDRLSAYYKKPEECTIAREFPGSELVGLTYEPIFPWFADKKNPDPNDGPN
                     AHRGAFRVISGDFVSTEDGTGIVHIAPGFGEDDARVGRENGIPAVCPMDDEGRFTSDV
                     PDYAGQFVKDADKAIIDRLKAERKLIKRDQYVHNYPHCWRCDSPLVYRAISSWFVNID
                     KIKDDMLAANERINWTPAHLKHGRFGKWLEGARDWAISRNRYWGAPIPVWTCEDPACG
                     HDVCVGSVEELEKLSGQKVTDLHKHHVDKLTGACPKCGKTLRRTPEVLDCWFESGAMP
                     YAQAHYPFENKERFEATFPADFIAESLDQTRGWFYTLVVLAAALFKDTSFKNVVVTGL
                     VLAEDGKKMSKRLRNYPEVSEIFDSFGADALRIYLMNSALVKAEELNFSGHGVKEVLR
                     AFHLPLWNSYSFFVTYANVDGWDPTRDAVPLVSITNPLDRWILSATERLVGEVNEQMD
                     RYDLQRSITPLMGFLDELTNWYIRRSRRRFWKGTLDNEKKGAYTTLYRVLTRLSQVIA
                     PFTPFISEAIWRGLRSDAMSESVHLTDYPQADASLRDEALEQEMALAMRAVSMGR"
BASE COUNT          448 a          655 c          651 g          456 t
ORIGIN      
        1 cgccccatcg agaccgcccg catggccagg gccatctcct gctcgagcgc ttcatcccgc
       61 agggaagcgt ccgcctgcgg atagtccgtc aggtggacac tctcggacat ggcatcactg
      121 cgcagcccgc gccagatcgc ctcgctgata aagggtgtaa agggcgcaat cacctgggag
      181 agccgggtca ggacacgata gagtgtcgta taggcgccct tcttctcgtt gtccagtgtc
      241 cctttccaga agcgtctccg ggaacgccgg atataccagt tggtcaattc atcaagaaaa
      301 cccatcaagg gtgtgatcga gcgctggaga tcgtagcggt ccatctgttc attgacctcg
      361 cccacaagcc gctcggtcgc cgagaggatc cagcgatcaa gaggattggt gatcgaaaca
      421 aggggtactg catcccgggt cggatcccat ccgtcgacat tggcgtaggt cacaaaaaag
      481 ctgtaggaat tccagagcgg aaggtggaag gcgcgcagga cctccttgac cccgtgcccg
      541 gagaaattca actcctcggc cttgacgagg gccgagttca tcagatagat ccgcaaggcg
      601 tccgcaccaa agctgtcaaa gatctcggag acctccggat aattccggag ccgttttgac
      661 atcttcttgc cgtcctcggc aagaaccagt ccggtcacga ccacattctt gaacgaggta
      721 tccttgaaga gggccgcagc taggacaacg agagtgtaaa accagccccg tgtctggtcg
      781 agggactcgg cgataaaatc cgccgggaaa gtcgcctcga aacgctcctt gttttcaaag
      841 ggatagtggg cctgtgcgta gggcatggcc cccgattcga accagcagtc cagcacctcg
      901 ggtgtacgac gcagggtttt tccgcacttg gggcaggcgc cggtcagctt gtcgacatgg
      961 tgcttgtgca gatccgtcac cttctggccg gacagctttt ccagctcttc aaccgaaccg
     1021 acgcagacat cgtgcccgca ggccggatcc tcgcaggtcc agaccggaat gggcgcaccc
     1081 cagtagcggt tgcgcgagat ggcccagtcg cgtgccccct ccagccactt gccgaagcgg
     1141 ccgtgcttga ggtgggccgg tgtccagttg atccgctcgt tggcggccag catgtcgtcc
     1201 ttgattttat cgatgttgac aaaccagctc gagatggccc tgtagaccag cggcgaatcg
     1261 cagcgccagc agtgcggata attatggacg tactggtccc gcttgatcag cttgcgctcg
     1321 gccttgagcc ggtcaatgat ggccttgtcg gcatccttga caaactggcc ggcgtagtcc
     1381 ggaacatcgg acgtaaagcg gccctcgtca tccatcggac agacagcggg aattccgttt
     1441 tcgcgcccga cccgggcatc gtcctcgcca aatccgggag cgatgtgcac gatgccggtt
     1501 ccgtcctcgg tgctgacaaa atctcccgag atgacccgaa aggcaccgcg atgagcgttc
     1561 ggcccatcgt tggggtccgg attctttttg tcggcaaacc aggggaagat cggctcgtag
     1621 gtcagtccga ccagctcgct gccggggaac tcccgggcga tcgtgcactc ttccggtttt
     1681 ttgtaatacg ctgaaagccg gtccttggcc aggatgaagc ggtcctcgcc gtcaacgacc
     1741 tcgacgtagc ggatctcggc acccaccgcg agtgccatat tggagggcag cgtccagggc
     1801 gtggttgtcc aggccaggat gtatgtccgc tcccggccct cgagggcaaa gcggaccgtg
     1861 atcgccggat cggcgacttc tttgtatccc tgattggtct caaagttgga gagcggtgtc
     1921 gcgcaacgcg ggcagtaggg aaggatcttg aagctgcggt agatcagtcc cttgtcccag
     1981 agctgacgga agacccacat gatactttcc atgtaggaaa gatccatggt gatgtaggcg
     2041 tttttgaaat cgacccagcg acccatccgc tcgaccgtct tttcccactc gccgacgtag
     2101 cgcaggacaa tgctacggca ctcctcgttg aaacggtcaa tcccgaaatt ttcgatatca
     2161 cgttttgtct tgaggtcgag cttcttttcg atctcgtttt caaccggaag
//
LOCUS       DAOSHE010000380         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_49222_length_2196_cov_3.844465, whole genome shotgun sequence.
ACCESSION   DAOSHE010000380 DAOSHE010000000
VERSION     DAOSHE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49222_length_2196_cov_3.844465"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PLD82_09100"
     CDS             <1..1470
                     /locus_tag="PLD82_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOG65577.1"
                     /translation="VFSADDAEAWAAKGEKVILVRIETSPEDIGGMNVARGILTARGG
                     MTSHAAVVARGMGKCCVAGCGDLAIDYKTKKFTARGVTVKEGDWISLNGSTGVVYKDQ
                     IKTVEASLAGPFKTIMDLANKYRKLQVRTNADTPKDTAVAVKFGAEGIGLCRTEHMFF
                     EGDRIVSFRRLILVAEDVKRLRKDLELAEGDEKKKLEAELAPKLKQYNQALKALLPLQ
                     RKDFTGMFKELKGRPCTVRLLDPPLHEFLPQDDKGQKEMAKTMGVEPAVVKRVVEDLH
                     EFNPMLGHRGCRLGITYPEVTEMQVRAIIEAAIAVPGSKPEIMIPLVGNIKEFEDQKA
                     IALRVIDEVKKAKKVAKLDVSIGTMIEVPRAAMTADEIAKEAEFFSFGTNDLTQMGCG
                     FSRDDAGKFLKDYVSKGIYDYDPFEVLDQAGVGRLMQYAIEHGKKTRPNIKLGICGEH
                     GGEPTTVKFCSKIGLTYVSCSPYRVPIAILAAAQAAIEG"
     gene            complement(1682..>2196)
                     /locus_tag="PLD82_09105"
     CDS             complement(1682..>2196)
                     /locus_tag="PLD82_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65578.1"
                     /translation="SGFFNKLAAGLGLESMYADGYTIAAGEHAWNLVRIDGSLYPLDA
                     TWKRFIMHPEEMLASHWPAYREHQCISPPWPSYLTFLSRRGKSVSASQRSIFQKLRYT
                     SLQMCPYNPVIEVLRNGTWWTAFAKEIKNGMYHVHYLRAVWEMDEWVPASRVRPQPHT
                     IRNRNAGYVY"
BASE COUNT          489 a          625 c          628 g          454 t
ORIGIN      
        1 gttttctcgg ctgatgatgc cgaagcctgg gccgccaagg gcgaaaaggt cattcttgtc
       61 cgcatcgaga ccagtcccga ggatattggc gggatgaacg ttgcccgggg tattctgact
      121 gcccgcggcg gaatgaccag ccacgcggcc gttgtcgccc gtggcatggg caagtgctgc
      181 gtcgccggtt gcggcgatct cgcgatcgac tacaagacga aaaagtttac ggctcgcggc
      241 gtcactgtca aagagggcga ctggatctcc ctcaacggtt cgaccggtgt tgtctacaag
      301 gatcagatca agactgtcga agcctcgctc gccggcccgt tcaagacgat catggacctg
      361 gccaacaagt accgcaagct ccaggtgcgc accaatgcgg atacccccaa ggatacggct
      421 gttgccgtca agtttggtgc cgagggcatc ggactctgcc gtaccgagca catgttcttc
      481 gagggcgacc ggattgtctc cttccgccgc ctgatcctcg ttgccgagga tgtcaagcgc
      541 ctgcgcaagg atctcgagct ggccgagggt gacgaaaaga aaaagctcga agccgagctg
      601 gctcccaaac tcaagcagta caaccaggcg ctcaaggcac tcctgcccct ccagcgcaag
      661 gactttacag ggatgttcaa ggagctcaag ggtcgcccct gcacggtgcg tctgttggat
      721 ccacctctgc atgagttcct tccccaggat gacaagggcc agaaagaaat ggccaagacc
      781 atgggtgtcg agcccgctgt cgtcaagcgt gtggtcgaag atctccacga gttcaacccg
      841 atgctcggtc accgcgggtg ccgtctcggg atcacctatc ccgaagtgac cgagatgcag
      901 gtccgggcca ttatcgaggc cgccattgct gttcccggct cgaagcccga gatcatgatt
      961 ccgcttgtcg gcaacatcaa ggagttcgaa gaccagaagg ccatcgctct ccgtgtgatt
     1021 gacgaagtca agaaggccaa aaaggttgcc aaactcgacg tctccatcgg gacgatgatc
     1081 gaggtgcccc gcgccgccat gacagccgac gagatcgcca aggaagccga gttcttctcc
     1141 ttcggaacca acgacctgac ccagatgggt tgcggattct cccgtgacga tgccggcaaa
     1201 ttcctcaagg actatgtctc caagggcatt tatgattacg acccctttga agttcttgac
     1261 caggctggtg tcggtcgtct gatgcagtat gccatcgagc acggcaagaa gacccgcccc
     1321 aacatcaagc tgggaatctg cggcgaacac ggcggagagc cgacgaccgt caagttctgc
     1381 agcaagatcg ggctgaccta cgtcagttgc tcgccgtacc gcgttccgat cgcgatcctc
     1441 gctgcagcgc aggccgccat tgagggataa gggttcatta ccaggatgaa cgcagacgat
     1501 tcaaacactg cgttcattca atgttggccg caaggctgac tgaaacaaac gaagacacaa
     1561 gagtcgcccg caagggcggc tcttgctttt gtatcggtgg agaactcttg aaatgcacca
     1621 aactgaaaag ccttgccggc tgttaccgca tttcgtctgt ggttcgttcc gggaaataga
     1681 tctaataaac gtacccggcg ttccggttcc ggatggtgtg tggttgaggc cggacccggg
     1741 atgccggaac ccattcgtcc atttcccaga cggcccgcaa ataatgaaca tggtacatcc
     1801 cgtttttgat ttccttggca aaggcagtcc accatgtgcc attgcgcagt acttcgatga
     1861 ccggattgta cgggcacatc tgcaggctgg tataacgcag tttttgaaag atgctccgct
     1921 gggacgcact gacagacttc cctcttctgg acagaaaggt cagataggat ggccatggcg
     1981 gggagatgca ctggtgttcg cgataggctg gccagtggct tgccagcatt tcctcgggat
     2041 gcatgataaa acgcttccat gtagcatcca ggggatagag cgagccatca attctgacca
     2101 gattccaggc atgctcgccg gcggcgattg tgtatccatc tgcatacatg ctttcgagtc
     2161 ccagccctgc ggccagttta ttgaaaaatc ctgaat
//
LOCUS       DAOSHE010000381         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_49271_length_2195_cov_2.839720, whole genome shotgun sequence.
ACCESSION   DAOSHE010000381 DAOSHE010000000
VERSION     DAOSHE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49271_length_2195_cov_2.839720"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLD82_09110"
     CDS             <1..1373
                     /locus_tag="PLD82_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG65579.1"
                     /translation="WVVAYGGYEDEFFAPLHELTLAFFLSLGGVLILAALVITRVAAG
                     IARPVDAIGEELLRTATRLRETAGGISQSGRTIARAVNELMDSSFAMTGAADQLEQHS
                     TANRETTRLAANEVRSAAGTAHEGSRRAQSLEEAFLSIESKSGEASKVIGVIEAITFQ
                     TNLLALNASIEAARAGEAGKGFAVVAGEVKHLADKSGESARGSVEMIAGVQESVAQGR
                     NSVHDVAEGIGNVKNALDRINQLLSELDVSSAEQSGSTAHIVGGIRTINQAIQRIAAF
                     SGKNEEAGRELGEQSVALDGIITSLESVIGRGATAGARSEATRSVDVLMRWSDDFSVR
                     VGEIDGQHRHLVDLINELYQAMQAGRGDDVIGPILDELVAYAGAHFSLEEQYMERFKY
                     EGYAAHKRAHEAFVQKVLTVVEAFKAGRAGVTLDVLNFLRDWIVEHILKVDKKYSDCL
                     NTNGVV"
     gene            complement(1455..>2195)
                     /locus_tag="PLD82_09115"
     CDS             complement(1455..>2195)
                     /locus_tag="PLD82_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG65580.1"
                     /translation="FAHCNLACVYCQNNEISNRTLPRPAPLSIGEAVQRIEELLEHGA
                     RAVGFVSPSHQPRATAAIIDALRARGVRAPMIWNSSAYDEPSVLRAMEERIDIWLPDF
                     KYADPALAACLSQAADYPETALRALREMYRQKGSFLDTDDDGLAISGLIVRHLVLPGQ
                     VANSLACLDLLAQDLSPRVTVSLMAQYHPLPGMPAPLDRTLTLAEYHEVIECMHARGF
                     VNGWLQELDSHVEHLPDFANAADPFGVE"
BASE COUNT          425 a          632 c          764 g          374 t
ORIGIN      
        1 gctgggtcgt tgcctacggg gggtatgagg acgagttctt tgcccccctg catgagctga
       61 ccctggcctt ctttctctcc ttgggcggag ttctgatcct cgcggcgctt gtcatcaccc
      121 gggtcgcggc gggcatcgcc cgcccggtgg acgccatcgg tgaagaactc ctgcggaccg
      181 caacccgcct gcgcgaaacg gccgggggaa tcagccagtc aggtcgcacc atcgccaggg
      241 cggtcaatga attgatggac agttcgttcg ccatgacggg tgcggcggac cagcttgagc
      301 agcacagcac ggccaaccgc gagacgaccc ggctggctgc caacgaagtc aggagcgcag
      361 ccggtacagc gcatgagggg agtcgccggg cccagtcact cgaggaggct ttcctctcga
      421 tcgagagcaa gagcggcgag gcctccaagg tgattggcgt catcgaggcg attaccttcc
      481 agacgaatct cctggccttg aatgcctcca tcgaagcggc cagggccggt gaggcgggca
      541 aggggtttgc cgtggtggcc ggagaggtca aacacctggc cgacaagagt ggggagtctg
      601 cgcgggggag cgtggagatg atcgccggag tgcaggagag tgtggcccag ggacggaact
      661 cggtccatga tgtcgcggag gggatcggga atgtcaaaaa tgccctggac cggatcaatc
      721 agctcctctc cgaactggat gtttcctctg ccgagcagtc cgggagtacg gcgcacatcg
      781 tcggagggat ccgcaccatc aaccaggcca tccagcggat tgcggccttt tcggggaaga
      841 acgaggaggc gggacgcgag ctcggggaac agtcggtcgc cctcgacgga atcatcacct
      901 ccctggagtc ggtgattggc cgtggggcca cagccggagc caggtccgag gccacccgga
      961 gcgtggatgt cctgatgcgc tggtcggatg atttttcggt tcgtgtcggg gagatcgacg
     1021 ggcagcaccg ccatctggtg gatctgatca acgaacttta tcaggccatg caggccggac
     1081 gcggggacga tgtgatcggc ccgatccttg acgaactggt ggcctacgcc ggagcgcact
     1141 tcagccttga ggagcagtac atggagcgct tcaagtacga aggctatgcc gcgcacaaac
     1201 gggcccacga agcctttgtc caaaaggtcc tgacggtggt cgaggccttc aaggccggcc
     1261 gggccggggt gacgctcgat gtcctgaact tcttgcggga ctggatcgtc gagcacatcc
     1321 tcaaggtgga caagaaatat agcgactgtc tcaacaccaa cggggtggtc tgaggcgggg
     1381 gacgggtcac atcggtttgt gacagacccc acccggcccg aggtcgttcc ccatacatca
     1441 ggccaggcag gccatcactc aaccccgaag ggatcggccg catttgcaaa gtccggcaaa
     1501 tgttccacat gactgtccag ttcctggagc cagccattga cgaacccgcg ggcgtgcatg
     1561 cactcgatga cttcatgata ttccgccagc gtcagggtcc ggtcgagcgg ggccggcatc
     1621 ccggggaggg gatggtactg ggccatcaag gagacggtca cccggggtga gagatcctgt
     1681 gccaaaaggt cgagacaggc cagcgaattg gcgacctggc ccgggaggac gagatggcgc
     1741 acgatcaggc ccgagatcgc cagcccgtca tcgtccgtat cgagaaagga gcctttctgg
     1801 cggtacatct cgcgcagggc ccgcagggcg gtctcgggat agtccgctgc ctgcgagagg
     1861 caggcggcca gggcagggtc ggcgtattta aaatcgggca gccagatatc gatccgttct
     1921 tccatggcgc gcaggacgga gggctcgtca taggccgagc tgttccagat catgggagcc
     1981 ctgaccccgc gggcgcgcag tgcgtcgatg atggccgctg tggcccgcgg ctggtgcgag
     2041 ggcgagacaa agccgacggc ccgggcaccg tgctcaagca gctcttcgat acgctgtaca
     2101 gcttccccga tagaaagggg ggcagggcgg gggagggtgc gattgctgat ctcattgttt
     2161 tggcaataga cgcaggccag gttgcagtgc gcaaa
//
LOCUS       DAOSHE010000382         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_49302_length_2194_cov_3.290790, whole genome shotgun sequence.
ACCESSION   DAOSHE010000382 DAOSHE010000000
VERSION     DAOSHE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49302_length_2194_cov_3.290790"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLD82_09120"
     CDS             complement(<1..1171)
                     /locus_tag="PLD82_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65581.1"
                     /translation="MNTNRQNLSVPSPSGPARLEIYSSAYTLSDMEIFVFPQLLYSLM
                     LANIMSERLWAWRRDPWFDGIESMTPEKRVMRLKQFVMDRYTFNLDLETWGLTTKDAE
                     MARFRDYIDESILRESNALFGYEGDKYYFSIDIRRHFGLDKYTTEFIPYWKTETMQAM
                     DAFVRKPGWRTGGGECVSLAALYAAALFVVARIPLDDIWLFATPLHSQNYIDTGDGIL
                     TNNRRVVTKVMFFNGSELSQKARRALENEKITVVAHRTGWIHTIYPEATIDMEAYGRF
                     GQRLSRYLRSPFTPEVFGNFIRCHPQYQPCFSYRAKGEGASDLYIECEKALRYEESTK
                     LRVSDQSRKKLLGLMPNLVTAVDFRGRCTASDVATSIVGSTWRRDRRRTPDEIRKP"
     gene            1278..>2194
                     /locus_tag="PLD82_09125"
     CDS             1278..>2194
                     /locus_tag="PLD82_09125"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOG65582.1"
                     /translation="MIHLPRLAALVSACLVLAAAWQGIPPGSSFLTGPVHGIPAPLPD
                     EAMVTLPATDDDEHEPDLATDTGHFREVSTVLSNGPVNRSVDIVVTGDGYTRAELAHG
                     GLFDQHAREFIRNFFNEPPFSELKRLCNVHLVRAISQESGADQNQHEDLKDTAFDAAY
                     GAYGIDRLLVIRRPEKLRRTVLLAPAQDIVVVLVNDARYGGSGSMIPAGTGSIPAPVY
                     AARNSSGIRIALHELGHSLANLADEYVDESIAAHYSLSYVADKPNVDTTSDLKKIKWN
                     AFYEEEPAATNLIKAWEGGYYRTRGIWRP"
BASE COUNT          494 a          660 c          593 g          447 t
ORIGIN      
        1 ccggcttccg gatctcgtcc ggcgtccggc gcctgtcgcg ccgccatgtc gatcccacaa
       61 ttgacgtcgc aacgtcagac gccgtacatc gccccctgaa atcaaccgcc gtcacgaggt
      121 tcggcatcaa ccccaaaagc tttttccggg actggtcact caccctgagc ttggtggatt
      181 cttcatagcg cagggccttt tcacactcga tatagagatc gcttgcgccc tcacccttgg
      241 cccggtagct gaaacagggc tggtattggg gatggcatcg gatgaaattc ccgaagacct
      301 ccggtgtaaa tggggaacgg agatagcgcg agagtcgctg cccgaaccgg ccataggcct
      361 ccatgtcgat cgtcgcctcc gggtagatcg tatggatcca gcccgtcctg tgggccacta
      421 ccgtgatctt ctcgttttca agggctctgc gcgccttctg ggaaagctca ctcccgttga
      481 agaacatgac cttggtcacg acccggcggt tattggtcag aatcccgtca cccgtatcga
      541 tataattctg cgaatgaagc ggtgtggcaa agagccagat gtcatccagg ggaatcctgg
      601 ccacgacaaa gagggctgca gcatagaggg ccgcaagact gacacactct ccgcccccgg
      661 tccgccagcc cggtttccgg acaaatgcgt ccatggcctg catggtctcg gtcttccaat
      721 atggaatgaa ctccgtcgtg tacttgtcga gtccgaaatg gcgacggatg tcgatggaaa
      781 aatagtactt gtccccttcg tatccgaaaa gggcgttgct ctccctgaga atcgattcgt
      841 cgatataatc ccggaagcgg gccatctcgg cgtcttttgt cgtcaggccc catgtctcca
      901 gatcaagatt gaaggtatac cggtccatca caaattgctt gagcctcatg accctttttt
      961 ccggagtcat ggattcgatc ccgtcaaacc agggatccct gcgccaggcc cagagacgct
     1021 cgctcatgat attagccaac atcagactgt agaggagctg aggaaaaaca aagatctcca
     1081 tatcggagag tgtgtaggcc gagctgtaga tctccagccg ggccgggccg gatggagagg
     1141 gtaccgagag gttctgtctg ttcgtgttca taattaaaaa gatatatagt aatgcactga
     1201 gaaacgaaga gaaaagcacg ggtgtattct ccagggggga ataccgatac tcacaggaaa
     1261 cccccgcgga gcgatcaatg atccacctgc ccaggcttgc agcccttgtt tcagcctgcc
     1321 tggtactggc ggcagcctgg caagggattc ctccgggatc ctctttcctg acaggtccgg
     1381 tgcatggcat ccctgcaccg ctccccgacg aagccatggt gacactcccg gcaacggatg
     1441 acgacgagca tgagccggac ctagcgaccg atacaggcca tttcagggaa gtgagcaccg
     1501 ttctctccaa cgggcctgtc aaccgttcgg tggacatcgt tgtaacggga gacggctata
     1561 cccgggcgga gctagcccac gggggactct tcgaccaaca cgcaagggaa ttcatccgga
     1621 acttcttcaa tgaacccccg ttttccgaac tcaaaaggct ctgcaatgtc cacctggtgc
     1681 gcgccatctc ccaggaatca ggcgcagacc agaatcagca cgaggatctc aaggacaccg
     1741 cctttgacgc ggcgtacggc gcctacggta tcgaccgact ccttgtcatc cgtcggcctg
     1801 aaaaactccg tcgaaccgtt cttctcgcgc cggcccagga cattgttgtc gtccttgtca
     1861 acgacgcacg ctatggcggc tccggctcga tgatcccggc cggaaccgga tccatccccg
     1921 cccccgtcta tgcggcacgc aattccagcg gaatccggat cgccctgcat gaactgggac
     1981 actccctcgc caatctcgct gacgaatatg tcgatgaatc gatcgctgcc cactacagcc
     2041 tgagctatgt tgccgacaag ccgaatgtgg acaccacatc cgatctgaaa aaaatcaaat
     2101 ggaacgcctt ttacgaagaa gagcccgcag ccacaaatct gatcaaggcg tgggaaggcg
     2161 ggtattaccg gacgcgcgga atctggcgac cgga
//
LOCUS       DAOSHE010000383         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_49722_length_2181_cov_3.634525, whole genome shotgun sequence.
ACCESSION   DAOSHE010000383 DAOSHE010000000
VERSION     DAOSHE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49722_length_2181_cov_3.634525"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..597)
                     /locus_tag="PLD82_09130"
     CDS             complement(109..597)
                     /locus_tag="PLD82_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOG65583.1"
                     /translation="MKRLVVCSAVLFVVAGCSSSASRDKDSVAGVGLANPAAEYCTAQ
                     GGKFEVRPSDGGEAGFCVVNGKAWEAWSFFRGEVGPGIEPVVVQPALAGQMKTIRPCK
                     AWSRPERVAQPVKELAANESVVVKKAVRYKNVVWTQVEISMGSYTVPGWLEGDPQAVV
                     RK"
     gene            complement(665..>2181)
                     /locus_tag="PLD82_09135"
     CDS             complement(665..>2181)
                     /locus_tag="PLD82_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG65584.1"
                     /translation="RSSIAGSRSPPRGSSPPSWIYNQINGGQNNGGYIHSALDLLKIR
                     GGATLATMPYTTDYRKTPPSGALQEAARYRNREYYSVRSLVAIKQALAQGHPVVVGIV
                     TDKEFMSGRFDIYNQAIRSRATRDPLNAHGRHAMVILGYDDIRGALLFLNSWGTAWGK
                     EGYAWIGYDVIGTIAYDSQERNFMECAYVMIDDRSTDVVQPGNRDFEVIGRSVSGGLS
                     AGVPAWNLRAHIKADSAILDTIRQVSWQVPDGFIVADGTVKTSAEDNFEIVGRLRGTG
                     TRMIRVVVSFRDGTSATKSFTWNLAAATRGALVLEQTDKYYGKIGRKEYWNWWLKIRG
                     SLNDLEDIQDVTYHLVSTYPDPDPVVRGTAANGFSYSARGWETFVVRATVRFKDGTTR
                     ELQTRLVFKDRIQQSLVLKNTAVPYREDDDGRTWYSWTAFIDGPASDLSRVEKVVYYL
                     HPSFRNNVHAVTSGAGYGFPFSATGWGTFVLRAKVHMNDGSVRDLSYRLDFTQQ"
BASE COUNT          468 a          690 c          579 g          444 t
ORIGIN      
        1 aagccacacg cggacagaca ccaagcacat ccgacgtgcg gcaatcgatc cttgtcactg
       61 cacggcacca cgtattgccc gccggatgaa caacgcgtgg cagcattgtc acttgcggac
      121 gaccgcctga ggatcaccct cgagccagcc ggggacggta tacgaaccca tcgaaatctc
      181 gacctgggtc cagaccacgt ttttgtagcg tacagccttt ttcactacga ctgattcatt
      241 ggccgcaagc tccttgacag gttgtgcgac acgttcggga cgggaccacg ccttgcaggg
      301 cctgatggtt ttcatctggc ctgccagggc cggctgcacg acaaccggct cgatacccgg
      361 cccgacctca cccctgaaaa acgaccaggc ctcccatgcc tttccattga ccacgcaaaa
      421 accggcctcc cccccgtccg aagggcgcac ttcaaacttc ccgccctgtg ctgtgcagta
      481 ctccgccgcc ggattggcaa gtccgacacc ggcaacagaa tccttgtccc gtgaagcaga
      541 agaggagcag ccagcaacca cgaacaaaac agcactacac acaaccagtc ttttcatacg
      601 ttcattcctt ttcctcaggc tgtcaatgac agcgtcgttg caaatcttta gacaaacagt
      661 aattctattg ctgcgtaaaa tcaagtcgat acgagagatc ccgcacgctt ccgtcattca
      721 tgtggacttt ggcccgcagg acaaaggtcc cccagccagt cgccgaaaag gggaagccgt
      781 accccgcgcc gctggtaacg gcgtgcacat tgttgcggaa tgagggatgg aggtagtaga
      841 ctaccttctc gaccctggac aggtccgaag ccgggccatc gataaaggca gtccagctat
      901 accatgtcct cccgtcatca tcctctcggt aggggactgc cgtgttcttg agaaccagac
      961 tttgctggat cctgtctttg aagacgagcc gggtttggag ctcccgggtt gttccgtcct
     1021 tgaagcggac ggtggctctg acaacgaacg tctcccaccc cctggcacta tagctgaacc
     1081 cattggcggc cgtccctcgt acaaccggat ccggatcggg gtaggttgag acaagatgat
     1141 aggtcacatc ctgaatatct tccagatcat tgagactccc acggattttc agccaccagt
     1201 tccagtactc cttgcgtccg atttttccat agtacttgtc tgtctgctca agcaccaggg
     1261 cccctctggt tgccgctgca agattccagg taaaactctt tgtcgcactc gtcccgtccc
     1321 tgaagctgac cacgacccgg atcatccggg tacccgtacc gcgcaaacgg cccacgattt
     1381 cgaaattgtc ctctgccgat gtcttgacgg tgccgtcagc cacaataaac ccgtcgggga
     1441 cctgccatga gacctgccgg atcgtgtcca agatggcact gtcggccttg atatgggccc
     1501 gcaggttcca ggccggcacg ccagccgaga gtccaccgga aaccgagcgc ccgatgacct
     1561 cgaaatccct gttccccggc tgcaccacgt ccgtcgaacg gtcgtcaatc atcacgtagg
     1621 cgcattccat aaaattgcgt tcctgtgaat cgtaggcgat ggttccgatc acatcatagc
     1681 cgatccaggc atagccttcc ttgccccagg cggttcccca tgaattgaga aagagcaggg
     1741 ccccgcggat gtcgtcatag ccaagaatca ccatcgcatg ccggccgtgg gcattgagcg
     1801 gatcacgcgt ggctctggag cggatggcct gattgtagat gtcaaaccgc ccactcatga
     1861 actccttgtc ggtcacgatc ccgacaacaa ccgggtgacc ctgggccagc gcctgcttga
     1921 tcgccaccag cgaacgaacc gaatagtact cgcggttgcg gtagcgggcg gcttcctgca
     1981 gggcgcccga aggcggggtc ttgcggtaat cggtcgtata gggcatagtg gcaagcgtcg
     2041 ccccaccccg gatcttgagc aggtccagag cgctgtgaat atagcccccg ttgttctgcc
     2101 cgccattgat ctggttataa atccatgagg ggggagaaga tccgcgtgga ggtgaccggc
     2161 tcccagcgat gctcgatcgc t
//
LOCUS       DAOSHE010000384         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_50520_length_2157_cov_3.159848, whole genome shotgun sequence.
ACCESSION   DAOSHE010000384 DAOSHE010000000
VERSION     DAOSHE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50520_length_2157_cov_3.159848"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLD82_09140"
     CDS             <1..185
                     /locus_tag="PLD82_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65585.1"
                     /translation="LFALAAQATNTGHHGPMRIFARFSRRGWVAEVSREKNPDSDLPM
                     PKDVTWFYRVTSVTIH"
     gene            244..1302
                     /locus_tag="PLD82_09145"
                     /pseudo
     CDS             244..1302
                     /locus_tag="PLD82_09145"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
     gene            1534..2157
                     /locus_tag="PLD82_09150"
                     /pseudo
     CDS             1534..2157
                     /locus_tag="PLD82_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          478 a          640 c          567 g          472 t
ORIGIN      
        1 ggctctttgc actggcagcc caagccacca ataccggaca tcacggaccg atgcgcatct
       61 ttgcccgctt ttcccgccgg ggatgggtgg ccgaggtctc ccgggaaaaa aatccggatt
      121 cggatcttcc catgccaaag gatgtcactt ggttttaccg ggtcacatcg gtcacgattc
      181 attaacatcc cgaaatcttc ttcataaagg acaggcagta tgaaaatctc gaaacgcctc
      241 atgatggcac ttctggtatc gggtgtgaca ctgattgttt gtgttgtggg tctgcacctg
      301 agcccgatct tcggcaactg gtcaaacgac attctcgtca atactgtcgg tggagtcggc
      361 cgaagcgccg atccgggcgt cgtgctcctg ctgatcgatg aagacagcat cgcctatggt
      421 gaaaaactca aactcggtcg ctggccatgg ccgcgttcca tctacagcga gattcttgat
      481 ttcatcaaca aaacacatcc gcccaaggcc atttttttcg acatcctctt ctcggagtcc
      541 agccaggaaa ccaatcagga tgccgttttt tcagaggccg ttgccagatc cgggaatgtc
      601 gtccacaata tgctgtttgc acacaatacc gagcggacaa acacgatacc actgccatcc
      661 gatgtagcga gccatttttc gatcgtggtc tcgaatgaac acgcagccgg tttcacatgg
      721 aaccaggcca accagtacac gattcctgtc cgggaattgc gcgcactgga cgaggccacc
      781 ccgatcgccc tcggtctggc gatggccgac ttctccccgg atccggatgg cgtctatcgc
      841 cgcggacgga tgctcttccg ctacggaagc ggagcggata cccgttacta cccctccctc
      901 tccctcaagg ccatcatgac actggttggc atcagtgctg tcacgcttga tggaaacaca
      961 attcatgtcg gaaaatattc cataccggtt gatgacaatg gaagaaccct tgtcaactat
     1021 cttcctgaaa gcctccccgc ctggagcatg agtgccttga tggaatccgc atggcacctc
     1081 gaacagggcg agccggaaaa gatgaagatt tctccggatg ccttcagcaa caagatcgtg
     1141 atcatcggag tctcggccgt tggcgggcag gatctcaaaa acacgccgat tgctccggcc
     1201 ctccccggtc ccgcaatcca tgcaaacctg atcagcgaca tcctccaaca aaaccacatt
     1261 acggtcgaac cacgctggat ctcctggctc ctcgtcctgc ttgttactgc gctctccatc
     1321 ggtgtcatgt tttccaaata ttcctccggc cgtcggattc tggtccaggc aggaacattg
     1381 ctcttgtacc tgctcctcgc cctgctcgcc ttcaggtttg gcaactactc atcccccctt
     1441 ctcgtgattg tgctgtccgg acttggggcg agcgccctcg gctggggata tgcaagcatc
     1501 accgaaggag cggaacgccg aaaatacagc aagatcctgg gaaacatggt cgacccgggg
     1561 attgtgcgcg aagccttgaa tgatcttgac gccctcaagc gcggtggcga aaaacagatc
     1621 acggctttct tttcggatgt tgcgagtttt tcgacgatca gtgaaaaact cgcaagtacc
     1681 gatctggccg ccctcttgaa cgagtatctc tccgagatga cactcatcct caaacgccac
     1741 ggcggcacgc tcgacaagta tatcggcgac gcggtcgtcg ggatctttgg agcacccgtc
     1801 gccatccccg agacggaact ggctgcggtg cgtgcctccg tcgagatgcg gaccagactg
     1861 gacgagatgc gtcgcgactg ggtcacgcgg aacgcctggt gccccgaagc acaggccatg
     1921 catttccgga tcggtttgaa ttccggtctg gccaaagtgg ggtttatggg aaccgacacc
     1981 ctggcgtcgt acacgatgat gggcgatacg gtcaatctcg cagcacgcct ggaagcggcc
     2041 ggcaaggact acggtgtcga aattcttgtc agcgagtcca tccagcaaaa tgccgaatcc
     2101 gagttcgaga tgcggaagat cgatctggtc cgcgtcaagg gaaaacacca gccagtt
//
LOCUS       DAOSHE010000385         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_50740_length_2151_cov_3.024809, whole genome shotgun sequence.
ACCESSION   DAOSHE010000385 DAOSHE010000000
VERSION     DAOSHE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50740_length_2151_cov_3.024809"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLD82_09155"
     CDS             complement(<1..463)
                     /locus_tag="PLD82_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOG65586.1"
                     /translation="MQNAWLTENPWWGGRYGGLLVAQPLLEYTGNDEVEVRVTNDMVF
                     RYLFGRPGSERILKSLINAVRVDAGLEPVAEVEVVDSATPEEFLDGKFSSVDVRARDT
                     VGRLINIEMQTWRQPGFVERSLYAWARAYSAQVVSGQNWKSLSPVACINILD"
     gene            complement(669..1745)
                     /gene="queA"
                     /locus_tag="PLD82_09160"
     CDS             complement(669..1745)
                     /gene="queA"
                     /locus_tag="PLD82_09160"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG65587.1"
                     /translation="MADQVSIPAGMSLDEYDFELPEAQIAAWPASERGRDRLLVMNRT
                     NGTLQHLEFACIESLLREGDVLVMNDVRVRKARLAATKDPGGGKVEILLLGEREDSGR
                     VWECLLHSTRAMRVGQVLQVQGGPQAVVTAREEERAILEFATPLNNAVLEEIGDIPLP
                     PYIMRQRASTVPLDSERYQTVYADRALAVAAPTAGLHFTDEILQAVCARGIMTEFVTL
                     EVGWGTFAPVRVDDIRQHRMHSERFEIAEDVAQRLMQARAKGRRIIAVGTTVVRALES
                     AARLDGSSLVIEPGIRSTDIFITPGYGFKAIDGMITNFHTPRSTLLVLVSAFAGREAI
                     RNAYETAVREGYRFFSYGDAMLIV"
     gene            complement(1730..>2151)
                     /locus_tag="PLD82_09165"
     CDS             complement(1730..>2151)
                     /locus_tag="PLD82_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65588.1"
                     /translation="SPWCAGTPHHSWVALIPASEAAQLLGLTGSVTLIEVRGWTPTQR
                     VRSLAVRSGTGQVRVLSGADFRRALGINRVKSMLFTPRVRGPWVQLVGRGWGHGVGMC
                     QWCASRMAQQGMEAVQILAHFYSGAAILPWRVVWPIK"
BASE COUNT          447 a          695 c          584 g          425 t
ORIGIN      
        1 aatccagaat attgatgcaa gcgaccggtg acagactctt ccagttttgc ccgctgacaa
       61 cctgtgccga atacgcccgt gcccaggcat agagactgcg ctcgacaaaa ccgggctgtc
      121 tccaggtttg catctcgata ttgatcagcc tccccacggt atccctggcc ctgacatcaa
      181 ccgaggaaaa ttttccatcg agaaactctt cgggggtcgc ggaatcgaca acttcgactt
      241 ccgccaccgg ttcaagaccg gcatccaccc ggactgcgtt gatgagcgac ttgaggatac
      301 gctcacttcc cggtcgccca aagaggtacc tgaacaccat gtcattggtt acccgcacct
      361 cgacttcatc atttccagta tactccaaca gcggctgtgc aacaaggagc cctccatatc
      421 ttccgcccca ccatggattc tctgtcaacc aggcattttg catcacgata tctccttcgg
      481 aggttgactt gcccccccta ctggaaatac acgtccgccc cccgatgcaa ttttgcataa
      541 aacgaatctt ttttcagtcc gtaaggtttt caggaatccg gaagcccagc cagaaactgc
      601 cggatccggc ccttatccag gcagaatccg gcagaggaac atgtttccct ggatggaatg
      661 ctgcgctctt acacaatcaa catggcgtcg ccgtaggaga aaaagcggta cccttcgcgg
      721 acagccgtct cgtatgcatt cctgatcgcc tcgcgtccgg caaaggccga gaccagtacc
      781 aggagggtcg atcttggcgt gtggaagttg gtgatcatcc cgtcgatggc tttgaatccg
      841 tagcctggtg tgatgaatat gtccgttgag cggatccccg gttcgatgac aagggatgaa
      901 ccgtcaagcc gtgccgcgga ctcaagagcg cggacaaccg ttgttcccac ggcaatgatc
      961 cgccggcctt tggcccgggc ctgcatcagt cgctgcgcca catcttccgc tatctcgaag
     1021 cgttcgctgt gcatcctgtg ctgacggatg tcgtccaccc gaacgggggc aaaggtaccc
     1081 cagcccactt cgagcgttac aaactcggtc atgataccac gggcacagac ggcctgcagg
     1141 atctcgtctg tgaaatgcaa cccggctgta ggtgcggcca ccgccagggc ccgatcggcg
     1201 tagaccgttt ggtagcgttc gctgtccagt ggaaccgtac ttgctcgctg gcgcatgatg
     1261 tacggtggga gcgggatgtc gccaatctct tccaggacag cattgttcag cggagtcgca
     1321 aactcgagta tcgcccgctc ttcttcacgg gcggtcacaa cggcctgcgg accgccttgg
     1381 acctggagaa cctggcccac ccgcatggcc cgggtggaat gcaggagaca ctcccagacc
     1441 cggccggagt cttcgcgttc gcccaagagc aggatctcga ccttgccccc cccggggtcc
     1501 tttgtggcag cgagacgggc cttgcgcacc cggacatcat tcatcacgag cacatcaccc
     1561 tcgcggagga gcgattcgat acaggcaaac tcgagatgct gcagagtgcc gtttgttctg
     1621 ttcatgacca ggagccggtc acggccacgc tcggaagcgg gccaggcggc gatctgcgcc
     1681 tcgggaagtt cgaaatcata ttcgtccagc gacattccag ccggaatgct cacttgatcg
     1741 gccacacgac cctccaggga agaattgcgg cgccgctgta aaaatgcgcg aggatctgga
     1801 ccgcttccat cccctgttgc gccatccggc tggcgcacca ctggcacatc cccacaccat
     1861 ggccccagcc ccttccgaca agctgcaccc atggtccgcg aacccgggga gtaaagagca
     1921 tgctcttgac ccggttgatc cccaaagcgc gcctgaaatc tgcgccactg agcacccgca
     1981 cctgccccgt gcccgagcgg acggcgaggc tccgcacacg ctgggttggt gtccagccgc
     2041 gcacctcaat caaggtgaca ctccctgtca atcccaaaag ttgggctgct tcgctggccg
     2101 gaatcaaggc cacccatgaa tggtgcggcg ttcccgcaca ccagggagag t
//
LOCUS       DAOSHE010000386         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_50936_length_2146_cov_3.395505, whole genome shotgun sequence.
ACCESSION   DAOSHE010000386 DAOSHE010000000
VERSION     DAOSHE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50936_length_2146_cov_3.395505"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLD82_09170"
     CDS             complement(<1..824)
                     /locus_tag="PLD82_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65589.1"
                     /translation="MPEYDGQKPAGPGTAKPTVSEQTPSVPDADSEPLLAEAPISVVK
                     LAFGHSEVSVFGLLAIFFNRARYQVLRTDTVVNFEARLFDVNPGIPWQDFERTIIDQL
                     TRQHLNFRLTNELRAKLDEHINYRKFEEFSALLRTHDHERLTIFLRAFLEKLYGVPGF
                     RTVLFLEEIFEDDFERRAPDEEVTPVAPEGEEEDVSSGTPFSPATPILSPVFGTVVAE
                     LEVGDSIWVRPQNPDAPDRKALSEEAVIRSITHDDSTGFTILAELGPGRIVRLIET"
     gene            complement(911..1312)
                     /locus_tag="PLD82_09175"
     CDS             complement(911..1312)
                     /locus_tag="PLD82_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG65590.1"
                     /translation="MNTRLSINTTSRDQMLDLTSEIQRLVTGSGVDRGFCYLFNPHTT
                     AALTINENADPDVMRDILAGLDRTIPWKADYRHGEGNSAAHIKASLFGPSLQIMVEHG
                     RLVLGTWQGIYFCEFDGPRQRHLEVRVYPVS"
     gene            1459..>2146
                     /locus_tag="PLD82_09180"
     CDS             1459..>2146
                     /locus_tag="PLD82_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65591.1"
                     /translation="MGLSDNEIRAAREKLRQQYDKLAAKYSPSMFNYARVDERYMQAL
                     RERLPVEHFLATEVRILKDLEKKAEEKFAPPPPPPGPSNADRMLEEFAQRISAWAPLE
                     FSDRADEETRRLAGALSEFEAEWGRAIPGVRALPPGTDAARLAAAVAERARTRLEPSR
                     SGGYPRALDDLALALARLTSGERERERLCKEYIKESGFLCNLVSDLFHELDAIGFAGG
                     YDYQAGAEAVD"
BASE COUNT          431 a          588 c          708 g          419 t
ORIGIN      
        1 tcggtctcga tcaggcgcac gatccggccc ggcccgagtt ccgcaaggat agtgaagcct
       61 gtgctgtcat cgtgggtgat gctgcggatc acggcctcct ccgagagggc ctttctgtcc
      121 ggagcatccg gattttgggg ccggacccag atcgaatccc cgacctcgag ttccgcaaca
      181 accgtcccga aaaccgggga gagaatcggg gtagccgggc tgaagggagt tccgctggat
      241 acgtcttctt cttccccttc gggggcgaca ggggtgacct cttcatccgg ggcgcggcgc
      301 tcgaagtcat cttcgaagat ctcttcgaga aagaggactg tgcggaatcc cgggaccccg
      361 tagagttttt ccaaaaaagc acggagaaag atggtcagac gttcgtggtc atgtgtcctg
      421 agaagggcgg agaattcctc gaacttccgg tagttgatgt gttcgtccag tttggcgcgc
      481 agttcgttgg tcaatctgaa attgaggtgt tgtcgggtga gctggtcgat gatcgtccgt
      541 tcgaagtcct gccaggggat gcccgggttg acatcaaaga ggcgggcttc gaagttgaca
      601 acggtgtcgg ttcgcaaaac ctgatagcgg gccctgttga aaaaaatcgc caggagacca
      661 aagaccgaga cctcggaatg gccgaaggcc agtttgacaa ccgagatggg tgcctcggcc
      721 aggagcggct cgctatccgc atcgggaacg gatggtgtct gctcactgac cgtgggcttt
      781 gcggtgccgg ggccggcggg cttttggccg tcatattcgg gcacggtgtc ctccagggtg
      841 gactcttgct ccagttgatc gagtatacac cgggtgagag gggagcggaa acggaaattt
      901 tcgggagaga tcaggaaacc gggtagaccc tgacctcaag atggcgctgg cgggggccgt
      961 cgaactcgca aaaatagatc ccctgccagg ttccaaggac cagtcgcccg tgttcgacca
     1021 tgatctgcag ggacgggcca aaaaggctgg ccttgatatg ggcggccgaa tttccttcgc
     1081 catgccggta gtcagccttc caggggatgg tccggtcgag tccggcgagg atgtcccgca
     1141 ttacatcggg atcggcattc tcgttgatcg tgagggccgc ggtcgtatgg ggattgaaga
     1201 ggtagcagaa accgcgatcg accccggagc cagtcacgag tcgctgaatc tcgctggtca
     1261 gatccagcat ctggtcgcgg gaagtcgtat ttatggagag tctcgtgttc atcagataaa
     1321 tatagtcacg aaagcgggaa ttatgggcag gggacccagg ccccccacac cgggaacccg
     1381 gccgggtttg tcggattctg tccgtcccct ccgcgttctc tacttgctcc cggagcgctt
     1441 tcacggtaga ctgccagtat gggactttcg gacaacgaga tcagggcggc cagggaaaaa
     1501 cttcggcagc aatacgacaa actggcggcg aagtacagcc cgtccatgtt caattatgcc
     1561 cgggtggatg agcgctatat gcaggccctg cgggagcgcc tgccggtcga acactttttg
     1621 gccaccgagg tgcggattct caaggacctc gaaaaaaagg ctgaagagaa attcgcgccc
     1681 ccgcccccgc ccccaggtcc gtccaatgcc gaccggatgc tcgaggaatt cgcgcagcgg
     1741 atcagcgcct gggcgcccct cgagttttcg gaccgggccg atgaagagac acgtcgcctg
     1801 gccggggcgc tcagcgaatt cgaggcggaa tgggggcgtg ccattcccgg cgtccgggcg
     1861 cttcctccgg gaacagatgc tgcgagactg gcagcagccg tggcggaacg ggcccggacc
     1921 cggctcgaac cttcccggag cggcgggtat ccacgagcgc tcgatgacct ggcactggct
     1981 ctggcccgac tgaccagcgg agaacgtgag cgggaacggc tctgcaagga atacatcaag
     2041 gagagcggat ttctctgcaa cctcgtttcc gatctgtttc atgaactgga tgcgatcggg
     2101 tttgctgggg ggtacgacta tcaagccggg gcggaggctg tagatg
//
LOCUS       DAOSHE010000387         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51019_length_2144_cov_2.730493, whole genome shotgun sequence.
ACCESSION   DAOSHE010000387 DAOSHE010000000
VERSION     DAOSHE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51019_length_2144_cov_2.730493"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLD82_09185"
     CDS             complement(<1..294)
                     /locus_tag="PLD82_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65592.1"
                     /translation="MLTAPVWFFISASLGTAAILVDWPFLSIPPFLVMLFVLYFFRAP
                     WRETPRDATLLYSSADGVVVRAGPCESPLHKGGRGQRVLVFMSPLDVHRNTAPL"
     gene            complement(336..1547)
                     /locus_tag="PLD82_09190"
     CDS             complement(336..1547)
                     /locus_tag="PLD82_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG65593.1"
                     /translation="MDLFEKCTASPVAKHARDFMERGIYPYFRPISTGQDTEVWIEGK
                     KYLMLGSNSYLGLTSDPRVIAAANKATLKYGTGNAGSRFLNGTLDIHEALESKIAALL
                     GRESAILYSTGYQTNLGVISALVGPDDVVVTDKADHASIIDGCKLSFGEMLRFRHQDM
                     AHLESVLAKIPEDKGILIVVDGVYSMEGDIADLPGIVPLARKYGARIMVDDAHAIGVI
                     GKGGRGTASHWAELGKIKEQDVDIVTGTFSKSWASLGGFCACSRDVMHYMRHVSRSLI
                     FSASMTPGTVAAVSKSIDIMLDEPERITNLWAITRMMQDGLTRMGFGIGHSETPIVPI
                     HIGQMEDAFRMWRGLTDEGLFVNPVVPPAVSPGDCLIRTSYMATHTKEQMTMALEKIR
                     KVGRGLGLVRE"
     gene            1683..>2144
                     /locus_tag="PLD82_09195"
     CDS             1683..>2144
                     /locus_tag="PLD82_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65594.1"
                     /translation="MRRILTILAIGLVLPATGLSAGMLTGVSAGGSWALAPGLAGSLD
                     GAGRVTLFTDFREPHWPASLGLALGYAPYTLSGVTGFSYRLTSLLARSWWAPPLGLPT
                     GLMLEAGLMAGAGFERLGDEERGSYGRSLLLGSGLRARIDLGGLETGLELDW"
BASE COUNT          467 a          651 c          597 g          429 t
ORIGIN      
        1 gagcggtgca gtattacggt gcacatccaa gggggacatg aacacgagga cccgctgccc
       61 acggcctccc ttatgcaacg gactctcgca aggccccgcc cgtaccacaa caccatccgc
      121 actggagtac agaagggttg catcacgggg cgtctcccgc cagggagcac ggaagaaata
      181 caggacaaag agcataacca gaaatggggg aatggagagg aaaggccaat ccacaagaat
      241 cgcggcagtt cccagagaag ccgatataaa aaaccagacc ggcgcagtca acatcgaacg
      301 ccacagggat cgcactgaat ggagaattgg ccggatcatt cgcgtaccag acccagtccc
      361 cggccaacct tccggatttt ctcaagcgcc atggtcatct gctccttggt atgtgttgcc
      421 atataactcg tccgtatcag acagtctccg ggtgaaacag cgggggggac gaccggattg
      481 acaaagagtc cttcatccgt caggccacgc cacatgcgaa aagcgtcctc catctgaccg
      541 atatggatgg gcacgatcgg ggtctcggag tgaccgatcc cgaatcccat tctggtcagg
      601 ccgtcctgca tcatccgggt gatcgcccag aggttggtga tccgctccgg ctcatcgagc
      661 atgatgtcga tggattttga tacggcagcc acggttccgg gtgtcatgct ggcggaaaaa
      721 atcagtgacc gggagacatg ccgcatataa tgcatcacat cccgggagca ggcacaaaac
      781 ccaccaaggg aggcccatga ctttgaaaac gtccccgtca cgatgtcgac atcctgttcc
      841 ttgatcttcc ccaactccgc ccagtgcgaa gcggtccccc ggccgccctt cccgatgacg
      901 ccgatggcat gagcgtcatc caccatgatc cgggcaccgt acttcctggc cagaggaaca
      961 atccctggaa gatcggctat atcgccctcc atggaataca ccccgtcaac cacgatcagg
     1021 atgcccttgt cctcggggat ctttgcaagc acactctcaa ggtgggccat gtcctgatga
     1081 cgaaaccgaa gcatctctcc gaacgagagt ttgcatccgt caatgatcga ggcatggtca
     1141 gctttatcgg tgacgaccac atcatccgga ccgaccagag ccgagatcac accgagattg
     1201 gtctgatatc cggtgctgta gaggatggcc gattcccggc ccaaaagtgc ggcaatcttg
     1261 ctttcgagcg cctcgtgaat atcgagcgtc ccattgagaa agcgggaacc ggcatttccc
     1321 gtcccgtact tgagggtcgc tttattggcc gccgcaatca cccttggatc cgatgtcaaa
     1381 ccaagatagc tgttcgaccc aagcatcagg tattttttgc cctcgatcca gacctcggta
     1441 tcctgaccag tcgaaatcgg ccggaaataa gggtagatgc cccgttccat aaaatccctg
     1501 gcatgcttgg caaccgggga tgctgtacat ttttcgaaaa gatccatgga taggcctcca
     1561 ccatctgtac tgtattatat ggaaaataaa atggtaaagc cctggactcc agtgcgtcaa
     1621 gtacttccat tgataccatg agacttgcgc gggaatcttc cgtccgtgct atactccacg
     1681 gtatgcgacg cattctcaca atactggcca tcggtctggt cctgcccgca accggtctct
     1741 ccgccgggat gctgaccggc gtttcagccg gcgggtcgtg ggcccttgcc cccgggctcg
     1801 ccggttcgct ggatggggcg ggccgggtca cgctcttcac agactttcgc gagccccatt
     1861 ggccggcatc cctgggactg gccctcgggt acgcacccta caccctctcc ggagtgaccg
     1921 gtttcagcta ccgtctgacc agtctgctcg cccgttcctg gtgggccccg cccctgggcc
     1981 tgccgaccgg tttgatgctc gaagcgggcc tgatggctgg tgcgggcttt gagcgactcg
     2041 gcgacgagga gcgcggaagt tatggacggt ccttgcttct ggggagcgga ctccgtgccc
     2101 ggatcgatct cggggggctg gagaccggtc tcgaactaga ctgg
//
LOCUS       DAOSHE010000388         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51051_length_2143_cov_3.196839, whole genome shotgun sequence.
ACCESSION   DAOSHE010000388 DAOSHE010000000
VERSION     DAOSHE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51051_length_2143_cov_3.196839"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLD82_09200"
     CDS             complement(<1..1071)
                     /locus_tag="PLD82_09200"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOG65595.1"
                     /translation="MFSVEDVFAQRIGGKNFGKDTTIYKFEKIKRAKRAAAQEHPGVE
                     LLDMGVGEPDERADDLVIDTLAVEARDPANRGYADNGIREFQDAAALWLERVFGVTGI
                     DPATEILHSIGSKPSLAMLPHVLINPGDVALMTVPGYPVFGTYTKYLGGEVYNLPLVA
                     ERGFLPDLTAIPAEILKRAKVLVLNYPNNPAGRGATEEFFRSVVEFAQKNNIVIIHDA
                     AYAALVYDRKPLSFLSIPGAKEVGLEIHSLSKAFNMTGWRMAFVAGRKELVAAYGTVK
                     DNTDSGQFIAVQKAAAVALAHPEITERTRVKYERRLVAMVETLKKLGFEARMPDGSFF
                     LFVRAPKGTADGKSFANAEEFSQ"
     gene            complement(1149..2030)
                     /locus_tag="PLD82_09205"
     CDS             complement(1149..2030)
                     /locus_tag="PLD82_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65596.1"
                     /translation="MKLRFDPAALLRLVFLGAGIAIAVFVLQMIEDGEIGMAAVVGIL
                     LLIGAGGVALMLFRLPLYAFFGMNAAGAKLTGRVLAGMAEDAPGRDLFLYQHSYFLYG
                     MGHFREALEVLEEIDFGNIPQELQYVVMLNKGLIQNARFCPEEAISTLEQISFEDRAQ
                     GDLYANWLAYMAEAWAQLGRELDQALDMAERAFSVTPSPRIAVILGHILVRMKQYQAA
                     EAWITWGIKRLRRKERYFGSYARYLRALLVRATGKGEEGEKAVLKALAQAPSEECRDI
                     FRKGWKLRGPARKRRQP"
BASE COUNT          444 a          685 c          599 g          415 t
ORIGIN      
        1 ctgcgaaaac tcctcggcat tggcaaagct cttcccgtcg gccgtcccct tgggcgcccg
       61 gacaaacagg aagaaagacc cgtccggcat cctggcctca aaaccgagtt ttttaagggt
      121 ctcgaccatg gccacaagcc gtcgctcgta cttgacccgc gtgcgctcgg tgatctccgg
      181 gtgagccagg gcaaccgccg ccgccttttg aaccgcgata aactgcccgg aatcggtatt
      241 gtccttgacg gttccgtagg ccgccacgag ttccttccgg ccggccacaa aggccatgcg
      301 ccagccggtc atattgaagg ccttggagag ggagtggatc tccagtccga cctctttggc
      361 tcccgggatc gagaggaagg aaagcggctt gcggtcgtag accagggcgg cgtaggccgc
      421 atcatgaatg attacaatat tgtttttctg tgcgaattcg accaccgagc ggaaaaactc
      481 ctcggtcgcc ccgcggccgg ccggattgtt cgggtagttc aacaccagaa ccttggcccg
      541 cttgagaatc tccgccggga tcgctgtcag gtcgggcaaa aacccgcgct cggccaccag
      601 cgggagattg tagacctcgc ccccaaggta ttttgtgtag gttccaaaca ccggatagcc
      661 ggggacggtc atcagggcca catcgcccgg attgatcagg acgtggggca gcatggccaa
      721 tgagggcttg gacccaattg aatgcaggat ctcggttgcg ggatcgatcc cggtcacgcc
      781 gaagacacgc tccagccaga gggccgccgc atcctgaaac tcacggattc cgttgtccgc
      841 atagccgcgg ttggccggat cgcgagcctc gaccgcaagg gtatcgatca cgagatcatc
      901 ggcgcgctcg tccggttcgc cgacacccat gtcgagcagt tcgacacccg ggtgttcctg
      961 tgcagccgca cgcttggccc gtttgatttt ttcgaatttg tagatcgttg tatccttgcc
     1021 aaaattcttt ccgccaatgc gctgggcaaa gacatcctca actgaaaaca tgcaacactc
     1081 ctcgattcaa cagagttcac caaaaaaaac atcaccggta tatgatcata aatatacgcc
     1141 ccggattctc agggctgacg ccttttccgc gcgggaccac gaagcttcca gcccttgcgg
     1201 aaaatgtccc ggcactcctc cgagggagcc tgcgcgaggg ccttcagcac ggccttctcg
     1261 ccttcctcgc ccttccccgt tgcacggacg aggagggccc gcagatagcg ggcatagctc
     1321 ccgaaatagc gctccttgcg acgcagacgc ttgatccccc aggtgatcca ggcttctgcc
     1381 gcctggtact gcttcatcct gaccagaatg tgtccgagga tgaccgcaat ccggggggaa
     1441 ggtgtgacgg aaaacgctct ctccgccata tccagggcct gatccagttc gcggccgagc
     1501 tgggcccacg cctcggccat gtaggccagc cagttggcat acaagtcgcc ctgggcacga
     1561 tcctcgaacg aaatctgttc gagcgtggag atggcttctt cgggacaaaa gcgggcattc
     1621 tggatcagcc ccttgttgag catcacaaca tattgcaatt cctgcgggat attcccgaag
     1681 tcaatctctt ccagaacctc gagagcctcc cggaaatgcc ccatcccgta caggaaatag
     1741 gagtgctggt acaaaaagag gtcccgaccc ggggcatcct ccgccatccc ggccagaacc
     1801 cggcctgtca gttttgcccc ggcggcattc attccgaaaa aagcatagag cgggagacgg
     1861 aagagcatca gggccacacc acccgctccg atgagcagga ggataccgac aaccgcggcc
     1921 atcccgatct ctccgtcctc gatcatctgc agcacgaaca cagcgatggc aattcccgca
     1981 ccgagaaaga ccagcctgag gagggccgcc gggtcaaaac ggagcttcac gcgcgattgc
     2041 cctcagtccg tttttttgtc gctggcgtcc tggcgcctgt cgcctggcgt tccgcactcc
     2101 gttcctcttt ggcagtcgca gcccgcgcag cctcaatccc ggg
//
LOCUS       DAOSHE010000389         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51081_length_2142_cov_3.331097, whole genome shotgun sequence.
ACCESSION   DAOSHE010000389 DAOSHE010000000
VERSION     DAOSHE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51081_length_2142_cov_3.331097"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..691)
                     /locus_tag="PLD82_09210"
     CDS             complement(86..691)
                     /locus_tag="PLD82_09210"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOG65597.1"
                     /translation="MRLIQRVLTAMIMLAALVACTGDGDPVLTSVEGAGWTSQLGATG
                     GIIEITFDKNDAAATGTMPAQKVNANNSVELFTCTYVSPGKSFAGWATSPLGAVIYAD
                     KAPITLGTNSFTLYARWNCVVSFDANGGTTPVPATNMIVLFGGPYGSLATTSRAGYAF
                     AGWWPEAGGAGFRPDHGQYHHGQSRSYSLGEVVAVGLHGQL"
     gene            complement(703..1503)
                     /locus_tag="PLD82_09215"
     CDS             complement(703..1503)
                     /locus_tag="PLD82_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65598.1"
                     /translation="MDRTPLVNRLVSEDGDRKNVLLWQWLNDLTGDNAAVVLTQAGDL
                     AKVSLPRMWRKGQTIGQVVRGAQLFLGDFKVHFSQRTLTLISMTASEKMNNEDYRKTV
                     FASGWGEGLVFSVEAWGGLMQPATVAAEADPFTSFGAGLALGFMPGLEATLRFESGSG
                     EMVADGKRLEATKLNLLAGLRWRPFADWFVSPYLAGSALFSVYSEKLIVGGQEKSWSG
                     AGFVAAAGLAVGPVAGFSLMIEYGRSWTFIPSNTLEFGELRAGVSLAL"
     gene            complement(1638..>2142)
                     /locus_tag="PLD82_09220"
     CDS             complement(1638..>2142)
                     /locus_tag="PLD82_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65599.1"
                     /translation="DGSIVKTLSGQMQSITALEFTPDGKQLVVSERGQHVIVYDTQTW
                     KSDKLIKAHTDVIWDVAVAPDGKSFATASKDRTIGIHRLDGTRLAEIGGFSGEIWTLA
                     WSPDGTKIACGLRTGELVIADVATKSIVRTLRSHETGIPAIAWSPTGRIVATGSRDGT
                     IRMWRGL"
BASE COUNT          450 a          680 c          598 g          414 t
ORIGIN      
        1 tccggcgcgg ctgacatccg gcaatggata caacgaaccg gaagtaacgg tcacgggatc
       61 aggtggggga atcccgccct gggcgttaaa gctgaccgtg tagtccaacg gcgaccactt
      121 cgcccagaga gtatgagcgg ctctgtccat gatggtattg accgtgatct ggccggaatc
      181 cggcaccgcc tgcttcgggc caccagccgg caaaggcata gcccgcccgc gaggtcgttg
      241 ccagcgatcc gtagggaccc ccaaacagca caatcatgtt ggtcgccggc accggcgttg
      301 ttccgccgtt tgcgtcaaag ctgacgacac aattccagcg ggcgtagagg gtaaacgaat
      361 ttgtccccag ggtgatcggc gccttgtctg catagatgac cgcacccagg ggactcgtgg
      421 cccagccggc aaagctcttt cccgggctga catacgtgca tgtaaacagc tcgacggaat
      481 tattggcatt cactttttgc gcgggcatcg tcccggtcgc agccgcgtcg tttttgtcaa
      541 acgtgatctc gatgatcccg cccgtcgcgc ccaactggct cgtccagcct gcgccctcga
      601 cacttgtcaa gaccggatcg ccgtcacccg tgcaggccac cagtgcggcc agcataatca
      661 ttgctgtcaa tactcgttgt atcagtcgca tcctgttccc ccttataatg ccaaagagac
      721 accagcccgc aactcgccaa attcgagtgt attggatgga atgaatgtcc atgagcggcc
      781 gtattcgatc atcagcgaaa agccggccac aggaccgacg gccagcccgg cagcggccac
      841 aaagcccgcc ccgctccagc tcttctcctg cccgccgact atcagttttt cggaatagac
      901 cgaaaagagg gctgaacccg caagataggg cgaaacgaac cagtcggcaa agggacgcca
      961 gcgcagaccg gccaggagat tgagcttggt cgcctcaagc cgcttgccat cagccaccat
     1021 ctctccgctg ccggattcaa aacgcagggt tgcctcaagg ccgggcatga atcccagcgc
     1081 cagccccgcg ccaaagctgg taaaggggtc cgcctccgcc gccactgtgg ccggctgcat
     1141 caagcccccc catgcctcga ccgaaaaaac caggccctcg ccccagccac tcgcaaaaac
     1201 ggtcttgcga taatcttcgt tgttcatttt ttcggaggcc gtcatcgaga tcagggtcaa
     1261 agtgcgttgc gaaaaatgga ccttgaaatc acccaaaaac agttgggcac cgcgcacgac
     1321 ctgtccgatg gtctggccct tgcgccacat ccgcggcagg ctgacctttg ccagatcccc
     1381 ggcctgggtc agcacgacag ccgcattgtc cccggtcagg tcattcaacc actgccagag
     1441 gaggacattt ttgcgatccc cgtcctcaga cacgagccgg ttgacgaggg gcgtccgatc
     1501 cacgggccca gccgcgaaag acgtgccagg aacagtgatg agcatgatca gcgtcatgat
     1561 ctgaatgggg aacagtctcc agccccggcg tatgcgtgaa ttcatggtat gtgatgtata
     1621 caatttcatg attttactca cagtccgcgc cacatgcgga tcgtaccatc acgcgagccg
     1681 gtggccacaa tgcgcccggt gggtgaccag gcgatggccg ggatacctgt ctcgtggctg
     1741 cgcagggtgc gcacgatgct ttttgtagcc acatcagcga tgaccaattc acctgtgcgc
     1801 aggccgcaag caatttttgt gccgtccgga gaccaggcca gggtccagat ctcgcccgaa
     1861 aacccgccta tctccgccag ccttgtaccg tcaaggcgat ggatcccgat cgtgcgatcc
     1921 ttggacgcag tggcgaaact ttttccgtcc ggcgcgacag ctacgtccca gatgacatcc
     1981 gtatgcgcct tgatcagttt gtcactcttc caggtttggg tatcatagac tatcacgtgc
     2041 tggccgcgct cggagacgac aagctgcttg ccatcgggcg taaactcgag ggccgtgatg
     2101 gactgcatct gcccgctcaa ggtcttgacg atgctgccgt cg
//
LOCUS       DAOSHE010000390         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51145_length_2141_cov_2.389741, whole genome shotgun sequence.
ACCESSION   DAOSHE010000390 DAOSHE010000000
VERSION     DAOSHE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51145_length_2141_cov_2.389741"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..663
                     /locus_tag="PLD82_09225"
     CDS             25..663
                     /locus_tag="PLD82_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65600.1"
                     /translation="MPRLNLVLAVFLVTSLSCTKKIPDEDLEAARIRSNVLSRASSAT
                     IPDWVLPGPACTISNALVITKVFVSRQDSLAAAETLARRETGVQTSEQIARWISTTSG
                     VTNLGLAATWCSNHAKVISRYWEAWRPEGRRDRLYTLYLQTAFDRKLLNQALSNEIRR
                     IHGTNALPVGLTNLFTMTAQDHRNSHPVVPVARHRLAVQAGRGQVCSNQHLS"
     gene            629..1051
                     /locus_tag="PLD82_09230"
     CDS             629..1051
                     /locus_tag="PLD82_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65601.1"
                     /translation="MDRFARTSIFPDGNCLPRKELSVPEQPEPRCLRPGNQGIALIKN
                     QPPLEHHPLDGQSFIKTLRFEITAADAGHEIRSLCGIQVRNQEEIASLQHKSIDGVLP
                     QDSLAENPLAIETDPGMIDDHLSLTGTDPQDTIVHSRA"
     gene            complement(1111..>2141)
                     /locus_tag="PLD82_09235"
     CDS             complement(1111..>2141)
                     /locus_tag="PLD82_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG65602.1"
                     /translation="VLSRAGFSGVSVVASQEQPDGNFPTVSRPNPEEEAAMKLAVLQA
                     VEENADLVLATDPDSDRVGVAVRDSTGTFVLLDGNQTGALLARHVFQGLARGQSNKQA
                     ACVSTVVSSELVRVMAEAAGIEYHSTLTGFKNIGSVMRMLAEERPESEFVFGFEESFG
                     YLFRDYIRDKDAVVACLMIAEAAAFARSHGESLWDLLIGIWHEHGVFREKLFSFEQEG
                     LAGAQRIERIMTLFRSPQSLNLFSGLGGTTLLDIRDRTMLDLTTGKLLPDTRYPVSNV
                     LVVSLPNNGKIAVRPSGTEPKIKFYISLSESCPAGSRQEDVLSVYQRLGREIDTLFAS
                     LEKVMEQA"
BASE COUNT          500 a          641 c          574 g          426 t
ORIGIN      
        1 tgagacatga atctggagaa cacaatgccc cgattgaacc ttgtattggc tgtgttcctg
       61 gtcaccagcc tctcctgtac caaaaagatc cccgatgagg atcttgaagc agccagaatc
      121 cgctcaaatg tcctctcccg cgcatcctct gcaacgatcc ccgactgggt cctgccgggg
      181 cctgcctgca caatcagcaa tgctcttgtc atcacgaaag tttttgtgag cagacaagac
      241 agtctggctg ctgcagaaac tctggccaga cgggaaaccg gagtccagac gagtgaacag
      301 attgcccgct ggatctcgac aacaagcggg gtgaccaatc tcggacttgc tgcaacctgg
      361 tgcagcaacc atgccaaagt catttcccgg tactgggaag cgtggcgccc agaaggccgg
      421 agggatcgtc tctataccct ttacctgcag acggcctttg accggaagct tctcaatcaa
      481 gccctgagta acgagatcag acggatccat gggaccaatg ccctccctgt tggattgacc
      541 aatctcttta ccatgacagc ccaggatcat cggaactctc atccggttgt gccagttgcg
      601 cggcatcgcc tcgccgtaca agccggacgt ggacaggttt gctcgaacca gcatctttcc
      661 tgatggtaac tgcctgcccc gaaaagaact ttccgttcca gaacagccgg agcctcgttg
      721 tctccggcca ggaaaccagg gcattgcgct gatcaaaaac cagccccccc tcgagcacca
      781 tcccctggat ggacagagct tcatcaagac tctccgcttc gagatcacgg ccgcggatgc
      841 gggtcatgag atccgctcgc tgtgcggaat ccaggtgcgg aaccaggagg agatagcctc
      901 tctccaacac aaatcgatag acggcgttct tccccaggat tccctggctg agaatccact
      961 cgccatcgag acagacccgg gtatgattga cgatcacctc tccctgacag ggaccgaccc
     1021 gcaggacact atcgtccatt cccgggcctg aggttgttcc ccacaaaaaa accattgaca
     1081 caaccagtgc tgtgaccggc cccctgcgtt tcatgcctgt tccatcacct tttcaagact
     1141 cgcaaagagt gtgtcaatct cgcgaccaag tctctggtac accgaaagga catcttcctg
     1201 acggctgccc gccggacagg actcggagag ggatatgtag aacttgatct ttggttccgt
     1261 accggacgga cggacggcga tcttgccgtt attgggcagg gaaaccacga ggacattgga
     1321 gaccggatag cgcgtatcgg gtagcagctt gcccgtggtc agatccagca tggtccgatc
     1381 gcggatgtcg aggagggtgg taccacccag tccggagaag aggttgagcg attgtggaga
     1441 acggaaaagc gtcatgatcc gttcgatgcg ttgtgcacct gcaaggccct cctgttcgaa
     1501 agaaaaaagc ttctcgcgaa agacaccatg ctcgtgccag attccgatga gaagatccca
     1561 gagactctcg ccatgactcc gggcgaatgc ggcggcctcc gcgatcatca gacaggcgac
     1621 aacggcatcc ttgtcccgga tgtaatccct gaagagatag ccaaatgatt cttcaaaacc
     1681 gaagacgaac tcgctttccg gacgttcttc ggccagcatg cgcataacgg aaccgatatt
     1741 cttgaacccg gtcagggttg agtgatattc aatcccggca gcctcggcca tgacacggac
     1801 cagttcactg gagacaacag tggaaacaca tgccgcctgt ttgtttgatt ggccacgtgc
     1861 cagcccctgg aagacatgcc gggccaagag agccccggtc tggttcccgt caagaaggac
     1921 aaatgtcccc gtcgaatcac ggacagccac gccaacccgg tccgaatccg gatcggttgc
     1981 cagcacaaga tcggcattct cctctacggc ttggaggaca gcaagcttca tggctgcttc
     2041 ctcttcggga ttggggcggc tgaccgtcgg aaaattgccg tcgggttgtt cctggctggc
     2101 aacaaccgag accccgctga acccggcccg ggaaaggaca t
//
LOCUS       DAOSHE010000391         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51397_length_2134_cov_3.585859, whole genome shotgun sequence.
ACCESSION   DAOSHE010000391 DAOSHE010000000
VERSION     DAOSHE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51397_length_2134_cov_3.585859"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLD82_09240"
     CDS             <1..963
                     /locus_tag="PLD82_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG65603.1"
                     /translation="GKSVCVNSIICSILYNMRPSQVRFILVDPKYVEMAPYNGIPHLL
                     IPVINKPRAAILALKWLVKEMEERYQCLQSLNARNIDSYNEKLAADPEAINRETGQPL
                     DRMPYLVLVIDELADLMLLAAKEIEDSISRLAAMSRAVGIHLIFATQRPSVDVLTGVI
                     KNNFPARIAFQVASTIDSRTILDTKGADKLLGKGDSLFVGPGSPAALRVQGAFLSEAE
                     VEKTADYLRSLGTPLYRMEILDEPDDRSDEDGDDFMAEDDPLYNEAIQIVIQTQRASA
                     SYVQRRLKVGYNRAARMIERMESEGIIGQQTGSKPRDVLVTHWP"
     gene            complement(1114..1839)
                     /locus_tag="PLD82_09245"
     CDS             complement(1114..1839)
                     /locus_tag="PLD82_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65604.1"
                     /translation="MEVWDGEHKSGEYKERIGAFSVGLVHRLGSGWEIGGRFEAQWYN
                     LDDGPYEGRHEGVPFGMTIGWNGQRVVEDRMQGFEWKIGALWDSGFFGSSLRRRAGTV
                     QWSLALPAGEGGFFVLEQSGAISDGLVPGLAYRLGGKGSQAVRQVRGYHEDQFRTTAW
                     MVTSVEWRLQVWTTQALLGSVFPFIDHGLFRDDGRARQILSVGLGFRVYLRKVLFPAF
                     SIYLAWAAENRELSGGVTAGMKM"
BASE COUNT          517 a          731 c          489 g          397 t
ORIGIN      
        1 ggaaaatcgg tttgcgtcaa ttccatcatc tgcagcattc tctacaacat gcgccccagc
       61 caggtccgct ttatcctggt cgacccgaaa tacgtggaaa tggctcccta caacggcatc
      121 ccccacctcc tgattcctgt gatcaacaaa ccccgggcag cgatcctcgc actcaaatgg
      181 ctcgtcaagg agatggagga gcgctaccag tgtctgcagt ccctgaacgc gcggaatatc
      241 gacagctaca acgaaaaact ggccgctgac cctgaggcca tcaatcggga aaccggccaa
      301 ccccttgacc gaatgccgta ccttgtcctc gtgatcgacg aattggccga cctgatgctc
      361 ctcgcagcca aggagataga agattcgatc tccaggcttg ccgccatgtc ccgcgccgtc
      421 ggcatacact tgatctttgc cacccagcgc ccctcggtcg acgtcctgac gggagtcatc
      481 aagaacaact tcccggccag aatcgccttt caggttgcct cgacaatcga ctcccgaacc
      541 atcctcgaca ccaaaggcgc agacaaactc ctcgggaaag gcgactccct ctttgtcgga
      601 cccggatcac cggccgccct ccgtgtccag ggcgcattcc tctccgaagc cgaggtcgag
      661 aaaaccgctg actacctgcg ttccctcgga acacccctct accggatgga gattcttgac
      721 gaaccggacg accggtccga tgaagacggc gacgatttta tggccgaaga tgacccgctc
      781 tacaacgaag ccatccagat cgtcatccag acccagcgcg cctccgcttc ctacgtacag
      841 cgacggctca aggtgggcta taaccgggcg gccaggatga tcgaacgcat ggagtccgaa
      901 gggatcatcg gccagcaaac gggatccaaa ccaagagacg tccttgttac tcactggccg
      961 taatccaggt gttttcatct ttgaggaacc tgttccagac aacaatgaac gctatgtaca
     1021 cgcaccatgg tacgttttcc ggctccagcc cgacgcgttc gatacccctg tcacggtctt
     1081 cagactgttc accataaaga ccatctatcc gagctacatt ttcatcccgg ctgtgacacc
     1141 accggacagt tcacggtttt cggcggccca agcaagatag atcgagaagg ccgggaagag
     1201 caccttgcgc aaatagaccc ggaacccgag tccgactgac aaaatctgcc tggcccgacc
     1261 gtcatcccga aagaggccgt gatcaataaa cggaaagacc gagcccaaga gagcctgtgt
     1321 cgtccagacc tgcaaccgcc actcaacgct tgtcaccatc caggctgtcg tcctgaactg
     1381 gtcctcgtgg tacccgcgca cctgccgaac agcctgcgac cccttaccac cgagacgata
     1441 ggccagaccg ggcaccagtc catccgagat cgccccgctc tgctcaagca caaagaaacc
     1501 gccctctccg gccgggagag ccagactcca ctggaccgtc cccgcacgcc tccggagtga
     1561 gcttccaaag aacccgctgt cccacaacgc accgattttc cactcaaacc cttgcatccg
     1621 gtcctcaacc acgcgttgcc cgttccatcc gatcgtcatc ccgaacggga caccctcatg
     1681 ccgtccttca taggggccat cgtcaagatt gtaccattgc gcctcgaacc tgcctccaat
     1741 ttcccaaccg ctcccaagcc ggtgaacaag cccgacagaa aacgcaccga tccgttcttt
     1801 gtactctccg ctcttgtgtt ccccatccca aacctccact cccctgccca tccactgggg
     1861 agaaaatgaa agaatccacg agcggctgga atccaggccc cattgggaaa agccaaagaa
     1921 gcccggtgtt ccgtcttgaa gaaagaaaaa accgcccaga gaattcagcg tacccaagaa
     1981 attgctttca agcacgccgg cccccagagt cagtttttct gataaacagc cacaaaggga
     2041 atcggtaaaa gtgtccattt ttcctggaca tcaagcacca gatccagacc atctcccttt
     2101 tccacaaccc ggacttccac ttttccgaag accc
//
LOCUS       DAOSHE010000392         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51496_length_2131_cov_3.736994, whole genome shotgun sequence.
ACCESSION   DAOSHE010000392 DAOSHE010000000
VERSION     DAOSHE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51496_length_2131_cov_3.736994"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2131)
                     /locus_tag="PLD82_09250"
     CDS             complement(<1..>2131)
                     /locus_tag="PLD82_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG65605.1"
                     /translation="VPEPPSRIHPAIPPAVEKIVLRLVEKEPERRYQSARGLLADLAR
                     VLAGEENFLPGRDDVSVRFSFQTGLIGREAELATLLRCHDNVREGRGRVCLVAGEAGQ
                     GKTRLVEEFSRCTLDRGTMVLTGKSSAGGNTVPFGMLREIVEQYLDAWERKNEGDRAM
                     TGARLREMLGAPATLVTSLHAGAARLFGDLPPLVPLDSDRENKRFLATMAAFFCTLAM
                     VEGGLVLVLEDLHWIDQGSADILLEILDSCETTPLEIVGTWRDGEIDAHHPLVALLPD
                     SGRQDVVHLSCDAFDVQRMAALVGAVLHADPGTIGEIAEFVLNRSRGNPFFAVEILRQ
                     LLAGRVLASHGGLWRIDRRRLDSVSVSGTLLEMIMNRISALDPKEHLVLSHAAAIGRK
                     FGMQLLFSLFRDPPDMEPLSEQALVRVIDHAIEMQILEVLPGQDGVLSFVHDRIREVF
                     YRQADHEAARRLHANILRAMESGTSPGDDTDVASLFEMAHHAIEAGDADAILRLARPA
                     AEKARQAYANRDALRYHKAILAVLGEDAANQDHPGHSLWLESSMASGEVLLVTGQNDQ
                     AIACFENILAKKIVQDVRIEAHLALCRAWFRKGDWARCETMARKGLALLGEHLPARKF
                     SVGLGILREFAIHLLHLVFPFVFVRSTEKKDGKTARKIIEFYVNVGWSFILSDTTKFV
                     HSILRMVNLSRRKIGPSRELGVSLGSYA"
BASE COUNT          475 a          666 c          610 g          380 t
ORIGIN      
        1 gcataggatc cgaggctgac acccaactcc cgggaaggcc cgatcttgcg tcgggacaga
       61 ttgaccatgc gcagaatgga gtgcacaaat tttgtggtat ccgagaggat gaaagaccag
      121 cccacgttga cgtaaaactc gatgatcttt ctggccgtct ttccgtcttt tttctccgtt
      181 gaacgaacaa aaacaaatgg gaagacaaga tgcaggagat ggatggcaaa ctcgcgaaga
      241 atcccgagac cgaccgaaaa ttttctggcc ggaagatgtt ccccgagaag agccagaccc
      301 ttgcgcgcca ttgtctcgca gcgggcccaa tcccctttgc ggaaccaggc ccgacagagc
      361 gccaggtgtg cctcgatccg gacatcctgg acaatttttt ttgccaggat attctcgaaa
      421 caagcaatcg cctggtcatt ctggccggtc acgagcagga cctcaccgct ggccatgctg
      481 ctctcgagcc agagactgtg tccgggatgg tcctgattgg ccgcatcctc tccgaggacg
      541 gcaagaatcg ccttgtgata gcggagggca tcccgattgg cgtaggcctg gcgcgctttt
      601 tcggcagccg gccgggccag tctgaggatc gcgtcggcat cacccgcctc gatggcatga
      661 tgggccattt caaacaggga tgcgacatcg gtatcgtccc cgggcgatgt tccggactcc
      721 atcgccctca agatgttggc atgaagccgc cgggccgcct catgatccgc ctgcctgtaa
      781 aagacctcgc ggatcctgtc gtggacaaac gagagaacac cgtcctgccc tggcaaaacc
      841 tcgaggatct gcatctcgat cgcgtggtcg atgacccgca caagcgcctg ctcggagagt
      901 ggctccatgt ccggcggatc gcggaagagc gaaaacagaa gctgcatccc gaatttccgc
      961 ccgatggcgg ccgcatggga gagaacaagg tgttcctttg gatccaaggc ggagatcctg
     1021 ttcatgatca tctcgaggag tgttccggag acactgaccg aatcgagccg cctcctgtcg
     1081 atcctccaga gaccgccatg actggcgagc acccgcccgg ccaaaagctg cctgagaatc
     1141 tcgacggcaa agaacggatt tccacgactg cggttgagaa caaattcggc gatctcaccg
     1201 atggtaccgg gatcggcatg caagacagcc ccgaccaggg ccgccatgcg ttggacatca
     1261 aaggcgtcac aggaaagatg gacgacatcc tggcgcccgg aatccgggag cagcgcaacc
     1321 aggggatgat gggcatcaat ctcgccatcc cgccatgttc cgacaatctc gagcggagtc
     1381 gtctcgcagg aatcgagaat ctccagcagg atgtccgcgc tcccctggtc aatccagtgc
     1441 aggtcttcaa gcacaaggac cagtccgccc tcaaccatgg ccagggtgca aaagaacgcg
     1501 gccatggtcg ccagaaagcg cttgttctcc cgatcggagt cgaggggaac caggggtgga
     1561 agatcgccaa agagccgggc agcccctgca tggagactcg tcaccagagt tgcgggcgct
     1621 cccagcatct ccctgagccg cgcacccgtc atcgctcgat caccctcgtt ttttcgctcc
     1681 catgcatcga ggtactgctc gacgatctca cgcaacatcc cgaagggtac ggtattcccg
     1741 ccggccgagg attttcctgt caagaccatg gtgccgcggt cgagagtaca acgggagaac
     1801 tcctcaacca gacgggtctt cccctgtccg gcctcacccg caaccagaca gacccggccc
     1861 cgaccctccc gtacattgtc atggcagcgc aagagcgtcg cgagttcggc ctcccgcccg
     1921 atcaacccgg tctggaacga gaatctgacc gagacatcat cccgccccgg aagaaaattt
     1981 tcctcgcccg caagcacacg agccaaatca gcgagaagcc cgcgcgcgct ctgatagcgc
     2041 cgctccggtt ccttctcgac caggcgaagg acaatcttct cgacggcagg aggaatggcc
     2101 ggatgaatcc gtgatggagg ctcggggacg c
//
LOCUS       DAOSHE010000393         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_51932_length_2118_cov_4.178866, whole genome shotgun sequence.
ACCESSION   DAOSHE010000393 DAOSHE010000000
VERSION     DAOSHE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51932_length_2118_cov_4.178866"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>574)
                     /locus_tag="PLD82_09255"
     CDS             complement(<1..>574)
                     /locus_tag="PLD82_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG65606.1"
                     /translation="LSVHRAEQGDGKIFPLAGGAALIRDVLVREFLEHKGEFHGQEGV
                     QKIEKTGCGFVITTNSHNSSREYDAVICATGGYTWPKTGSTGDGYRFARELGHTIVPS
                     VPALTAIICPEEEIRGMSGVALRDVRVRIERAGMLLDVERAGDVLFTHRGLSGPAILD
                     ASYAMQRGDHVLIRCLDDERAAAMESGILE"
     assembly_gap    575..584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<585..856)
                     /locus_tag="PLD82_09260"
     CDS             complement(<585..856)
                     /locus_tag="PLD82_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG65607.1"
                     /translation="MKKNIAIIGAGPAGLMAAVVAGHEPDVRVVLYEKNPQPGAKLLI
                     SGSGQCNIASAGSLPERLRHYPDHVRDFLRPSLKAASLTRILDWFL"
     gene            940..1248
                     /locus_tag="PLD82_09265"
     CDS             940..1248
                     /locus_tag="PLD82_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65608.1"
                     /translation="MSVKFLSLLFAITLCITSCSSSRSELHVYNWTYYIPEEILKAFE
                     KETKIRLVYDVYTTNEEMYAKLKAGGDGYDVVSLPVITSQFSNPKTCFPRSITRFSRI
                     "
     gene            1209..1964
                     /locus_tag="PLD82_09270"
     CDS             1209..1964
                     /locus_tag="PLD82_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG65609.1"
                     /translation="MLSPLDHTLLPNLTNLNPVILSKITHDPKMRFSIPFVLGSSLIA
                     VNTRLYPDFEPSWRLFLKPELMQRTTMLDDMREVLGAALKSLGYSVNSTNEHELVAAK
                     QVVLAWKNNILKFDTETFAKSFAAGALFAAHCYPENIVQEYDPRRIEKDVTFFIPREG
                     GPMYVDNMCILAKSTRSSEAHAFINYLLRPEVYAKIIQRLRLPSINKNAEQYITNTPV
                     YSLDSLSNSEFIHDVGSAIISYNRIWQEIIIGK"
BASE COUNT          523 a          619 c          475 g          491 t
ORIGIN      
        1 ttttcgagga tcccggactc catggccgca gcccgctcat catcaagaca acggatgagc
       61 acatggtctc cgcgttgcat ggcataactg gcatcaagaa tggccggacc cgacaggccc
      121 ctgtgcgtaa acaggacatc gcccgcacgt tcgacatcca ggagcatacc agcgcgttcg
      181 atccggacgc gtacatcccg cagggccacg ccgctcatcc ctcgaatctc ttcctccgga
      241 cagatgatgg ccgtcaaggc cgggaccgat gggacaatgg tatgccccag ttcacgggca
      301 aaccggtagc catctccggt cgatcccgtc tttggccatg tgtacccacc ggttgcacag
      361 atgacggcat catattcccg agatgaattg tggctgttcg tggtgatcac gaatccgcat
      421 ccggtttttt ctattttctg aacaccctct tgcccatgga attcgccctt gtgttccaga
      481 aactcgcgca caagaacatc gcggatcagg gcagcaccgc ccgcgagggg aaatatcttt
      541 ccatcgccct gttccgcccg atggacggat aatgnnnnnn nnnnaagaga aaccagtcaa
      601 gaatccgcgt aaggctggca gccttgaggc tgggccgcaa aaaatcccgg acatggtccg
      661 gataatggcg cagccgctct ggcaaggaac cggcactggc aatattgcac tgacccgaac
      721 ccgaaatcag gagctttgca cccggctgcg ggttcttttc gtacagaaca acacgtacat
      781 cgggctcgtg tcctgcgacg acagctgcca tcaaaccggc aggcccagca ccaataattg
      841 cgatattttt tttcatgtca tccctgtcaa cgattgcact cactcctgtg aaggcgtaca
      901 ctgagagtgt cagtcaacgt atggcttcgg gggagtttca tgtcggtaaa atttctttct
      961 cttctttttg cgatcacgct gtgtatcacg tcctgcagtt cttcacgatc cgaacttcat
     1021 gtctacaact ggacctacta cataccggag gagattctca aagccttcga aaaagaaacg
     1081 aaaatccgcc tggtctatga tgtctacaca accaatgaag aaatgtacgc caaactcaaa
     1141 gcgggcggcg acggatatga cgttgtttcc cttccggtga ttacgtctca attctcgaat
     1201 cccaaaacat gctttccccg ctcgatcaca cgcttctccc gaatttgacc aatctcaacc
     1261 ccgtgattct ctcaaaaatt acacatgatc ccaaaatgcg cttcagcatt ccctttgtgc
     1321 tgggttcctc cctgattgcc gtcaatacca ggctctatcc ggattttgaa ccgagctgga
     1381 gattgttcct caaaccggaa ctcatgcaac gaacgaccat gctggatgac atgcgtgaag
     1441 ttcttggggc ggccctaaaa tctctggggt attccgtcaa ctccacgaac gaacacgaac
     1501 ttgttgcggc caaacaggtt gttctggcct ggaaaaacaa catactgaaa ttcgataccg
     1561 aaacttttgc caagagtttt gccgcaggag ccctttttgc cgcacattgc tatcctgaaa
     1621 acattgtcca ggaatatgac ccccgccgga tcgaaaaaga tgtcacgttt ttcattcccc
     1681 gcgaaggtgg tcccatgtac gttgacaaca tgtgtatcct ggccaaaagc acgcgctcat
     1741 ccgaagccca tgccttcatc aattatctcc tccgccccga agtctacgcc aaaatcatcc
     1801 agcgattgcg cctgccaagt atcaacaaaa acgccgaaca atacattaca aacacacccg
     1861 tctacagtct cgactctctc tcgaacagcg aattcatcca tgatgtggga agcgccatca
     1921 tcagctataa tcgcatctgg caagaaatca tcatcgggaa atgacaagaa agcccgggtt
     1981 ttccccgggc tttcttgtgt cacacacaat aacgatcaga gtcccttttt ggggagtatc
     2041 ctgacttttc gatggacacc ctcgccttca ctccgggtct cgaccgattt ctcgttttgc
     2101 agagcaacat gaatcaaa
//
LOCUS       DAOSHE010000394         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_52230_length_2110_cov_3.411679, whole genome shotgun sequence.
ACCESSION   DAOSHE010000394 DAOSHE010000000
VERSION     DAOSHE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52230_length_2110_cov_3.411679"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLD82_09275"
     CDS             complement(<1..769)
                     /locus_tag="PLD82_09275"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG65610.1"
                     /translation="MLDTPHFDPTITQLLPARFPSPLVTNFSTDSDRIILDVSVEAHE
                     KARTGEYDPILLELAGPREDLFFEPLDSRAAIVTCGGLCPGINDVIRAIVMNLWHTYG
                     CRDILGIRWGYEGLVERHRHEPLSLTPEVVDKIHEQGGSILGSSRGAQDIGEMVDFLE
                     NRGIDILFAIGGDGTLRAAHAIAQEILARGLKRSVIAIPKTIDNDINFVAKSFGFETA
                     FATAVTAIASAHTEAHAARNGIGLVKLMGRHSGFIAAN"
     gene            981..1580
                     /locus_tag="PLD82_09280"
     CDS             981..1580
                     /locus_tag="PLD82_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65611.1"
                     /translation="MWKRILTGMLAVFMACGVVYGFNDDDVGGERRVHIILHDSSDVE
                     WADLLVDGQSRGVVRKGSPLILTMQGGSSYLFRVTRTWNGRVYVREKLQRIEPGAGAQ
                     WIVLMPEIQDRDGAESRGHINITLPQASPVAWVNLAINDVAYGVMRRGETRRFWLKAG
                     MTHKIRLEREWKNKTWVFEHEVQVEDGQTVRLDARMQAE"
     gene            1650..1802
                     /locus_tag="PLD82_09285"
     CDS             1650..1802
                     /locus_tag="PLD82_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65612.1"
                     /translation="MDIDDILLEIGRETSARLIRPVRTRDVSAIPQAKTVVCGLETVP
                     HKDVVP"
     gene            1818..>2110
                     /locus_tag="PLD82_09290"
     CDS             1818..>2110
                     /locus_tag="PLD82_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65613.1"
                     /translation="MIWSGLDTMVLTPICALTVSKEAALLDLGISVEYGLEGRKRHYQ
                     EGVTVTMAGEIKYHIRLSSEMVEGATYAILPGDPGRVEKTARHIDPAGAKFLAA"
BASE COUNT          431 a          527 c          694 g          458 t
ORIGIN      
        1 cattggcggc gataaatccg gaatgacgcc ccatcagctt gaccaggccg atcccgttcc
       61 gggcggcatg cgcttcggta tgggcggaag cgatggcggt cacggcagtg gcaaaggcgg
      121 tttcgaagcc gaaggacttg gcgacaaaat tgatgtcgtt gtcgatggtt ttggggatgg
      181 cgatcacgct tcgtttcagt ccgcgtgcca ggatctcctg ggcgatggca tgggcggcgc
      241 gaagagtacc gtctccaccg atcgcgaaga ggatgtcgat cccgcggttc tcgaggaaat
      301 cgaccatctc cccgatatcc tgggcccccc gtgaacttcc gaggatggac ccgccctgtt
      361 cgtggatctt gtccaccact tctggggtca gggagagggg ttcgtggcga tggcgttcga
      421 ccagcccctc gtagccccag cggatcccga ggatgtcccg gcacccgtag gtgtgccaga
      481 ggttcatcac aatggcccgg atcacatcat tgatacccgg gcagaggccg ccacaggtga
      541 cgatcgcggc tctggaatca aggggttcga aaaagagatc ttcgcggggg ccggccagtt
      601 caagcagaat cgggtcatac tcaccggtcc gggctttttc gtgggcttcg acggagacat
      661 ccagaatgat cctgtcgctg tctgtcgaga aattcgtcac cagggggctc ggaaagcgtg
      721 ccgggaggag ttgggtgatt gtgggatcaa agtgcggcgt atcaagcatc tcgcaagcca
      781 cctccggaag catccgtttt gaatgcttga agtatatcct ttttttgcat gaagcgctac
      841 cggttatgct ttttggtccg atcaggacgg gatttatgca gcgagtgtgt gtcatttatg
      901 gaggaatggt gtagaccaaa gggaatgcga gttccaggtg cggtggcgtc tgttccaccg
      961 gggaatacgg ggtgagtgca atgtggaaac gaatcctgac aggtatgctg gccgttttca
     1021 tggcttgcgg tgtggtgtat ggtttcaacg acgatgatgt tgggggcgag cggagggtgc
     1081 acatcattct gcacgattcc tccgatgtcg aatgggcgga tcttttggtc gacgggcaat
     1141 cccgcggagt tgtccgcaag gggagcccgc tcatcctgac gatgcagggt ggatcaagct
     1201 atctctttcg ggtgacccgg acctggaatg gccgggtcta tgtccgcgaa aaactccagc
     1261 ggatcgagcc gggcgcgggt gcccagtgga tcgtcctcat gcccgagatc caggaccgtg
     1321 atggcgcaga gtcccgtgga catatcaata tcacgcttcc ccaggcatct ccggtcgcct
     1381 gggtcaatct tgccatcaat gatgtggctt acggggtcat gcggcggggt gagacgcgcc
     1441 ggttctggct caaggccggg atgacgcaca agatccggct ggagcgtgag tggaaaaaca
     1501 agacctgggt gttcgaacat gaagttcagg ttgaggacgg acaaaccgtg cgccttgatg
     1561 cgcggatgca ggccgaataa accgtgcgca ttcccggatt cttcgggata acgcaccatg
     1621 gtgtcggggc cgggttctgc agggtttgta tggatatcga cgatatcctc ttggaaatcg
     1681 gccgtgagac cagtgctcgc ttgatcaggc ctgtgcggac cagggacgtc tccgcaatcc
     1741 cgcaggccaa gacggttgtc tgtggactgg agactgttcc ccataaagac gttgttccat
     1801 agcatggaag agcaggaatg atttggtccg ggctggacac catggtgttg acgcccatct
     1861 gtgccctgac cgtatcaaaa gaagcggctt tacttgattt ggggatatcg gtagaatatg
     1921 gacttgaagg gcgaaagcgc cactatcagg aaggagtgac ggtaacgatg gcaggtgaga
     1981 tcaagtacca tatcagattg tcgagcgaga tggtggaggg tgcgacctat gcgatcctcc
     2041 ccggcgatcc gggacgtgtg gagaaaacgg cacgtcatat tgaccccgcg ggggcgaagt
     2101 ttcttgctgc
//
LOCUS       DAOSHE010000395         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_52872_length_2092_cov_3.926362, whole genome shotgun sequence.
ACCESSION   DAOSHE010000395 DAOSHE010000000
VERSION     DAOSHE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52872_length_2092_cov_3.926362"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLD82_09295"
     CDS             <1..976
                     /locus_tag="PLD82_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOG65614.1"
                     /translation="THADAPCAGPLAGFRDVKPMVFASIYPTKSEEYNSLKAAMEKLK
                     LNDASLVYEPDSSQALGFGFRCGFLGLLHLEIVQERLEREFDQDIIITSPTVEYEVYM
                     NNGKMEIIDNPVKFPDPQYIEHCAEPFVKATVITPAEFMGNIIPLCLEKRGTQRHMEY
                     LDEKRVEIIFDMPLAEIVYDFYDRLKSVSRGYASFDYEITSYEPAELVRVDILVNAKQ
                     VDALSQLVVRQGAALRGRQIVERLKDLIPRHQFKIPLQAAIGGSIIARETIGSVGKNV
                     TAKCYGGDISRKRKLLEKQKEGKKRMKMFGNVEIPQSAFTEVLKTPDK"
     gene            1206..>2092
                     /locus_tag="PLD82_09300"
     CDS             1206..>2092
                     /locus_tag="PLD82_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG65615.1"
                     /translation="MSAINWNEITNRAYAFVKEHEDMSSERSEAIVFWRDLLEVFGVK
                     ASLRNLFEHAVANLKGHYSRIDMFWEGVVLVEHKSRGKDLTRAESQAFSYIQDLARTE
                     PHKIPRYVILCDFANFLLYDLTQEPIEDHVRSFPLEDLPKHVKDFGFFINVDADRPLE
                     QEDLNLKAVELLGSLHDVIEDAKYPREHLDTYMMRLLFCLFADDTGILNHQMERVLQD
                     CNPDGAETGRIMQEMFDVLNTAKEARQSTMDSVLRELEYINGGLFNEHLPLLYYSREM
                     HQKLLECTRFDWGLISPVV"
BASE COUNT          475 a          547 c          578 g          492 t
ORIGIN      
        1 gacccacgcc gatgccccct gtgcagggcc gctggccggg tttcgcgatg tcaagccgat
       61 ggtctttgcc tcaatctatc cgaccaagtc cgaggagtat aattctctca aggcggcgat
      121 ggaaaaactc aagctgaatg acgcctcgct ggtctacgag ccggactcct cacaggcgct
      181 cgggttcggg ttccgctgcg ggtttttggg actcttgcat ctggagatcg tgcaggaacg
      241 gcttgagcgc gaattcgacc aggacatcat catcacctcg cccacggtcg agtacgaggt
      301 gtatatgaac aacggtaaaa tggaaatcat cgacaatccc gtcaaatttc ccgatccgca
      361 gtacatcgag cattgtgccg agccctttgt caaggccacg gtcatcaccc cggccgagtt
      421 catggggaac atcattccgc tctgcctcga aaagcgcggg acccagcggc acatggagta
      481 ccttgacgaa aagcgcgtcg aaatcatttt tgacatgccc ctggcagaaa tcgtctacga
      541 tttttatgac cgcctcaagt cggtcagccg gggctacgct tcctttgact acgagatcac
      601 atcatacgag ccggccgagc tggtacgggt ggatatcctt gtcaacgcca agcaggtgga
      661 tgcgctttcc caactcgttg tccgccaggg tgctgccctg cgcggccggc agatcgtcga
      721 gcggctcaag gacctgattc cacgccacca gttcaagatt ccgcttcagg ccgcgatcgg
      781 gggctcgatc atcgcccggg agacgatcgg gagtgtggga aaaaacgtga cggccaagtg
      841 ctacggtggt gacatcagcc gcaagcgcaa gctcctcgaa aagcagaagg aggggaaaaa
      901 gcgcatgaag atgttcggca atgtcgagat cccccagtcc gccttcaccg aggtactcaa
      961 gacgccggat aagtgagcgg gaagaacagg gctgtctggt ggtatcaatt ctacacagat
     1021 gctgtcatcg gcagcatcct tgtctttgcc cgatactgtc gggtgtcatt cattgtccca
     1081 tattccgctt ttgaaaaatg tgtctcttgc atccggcttg ttgtatgata catgggcaag
     1141 cattctccat ggtcaggggt gcaagaatgc ctgcctggat ttgtacccat agtaaggatg
     1201 ttatcatgag cgcgattaat tggaacgaga tcacaaacag ggcgtatgcc tttgtcaaag
     1261 aacatgagga catgtcctcc gagcggtcgg aggcgatcgt gttctggcgg gatttgctgg
     1321 aggtttttgg ggtcaaggca tccctccgta acctgtttga gcatgcagta gcaaatctca
     1381 aaggccatta cagccggata gatatgtttt gggaaggtgt tgttcttgtc gagcataaat
     1441 cccgtgggaa ggatttgact cgggcggaat cacaggcttt cagctatatc caggatctgg
     1501 ccaggacaga accgcacaag atcccccgct atgtgatcct gtgtgatttt gcaaatttct
     1561 tgctctatga tctgacgcag gaacccatag aggatcatgt ccgcagcttt ccgctggaag
     1621 atctccccaa gcatgtcaaa gattttggtt tttttatcaa tgtggatgcc gaccgtcctc
     1681 tcgaacagga ggacctcaac ctcaaggcgg tggagctgct cggctccctg catgatgtga
     1741 ttgaagatgc caagtatccc cgcgagcatc tcgataccta catgatgcgg ctcctctttt
     1801 gcctcttcgc cgatgatacc gggatcttga accaccagat ggagcgggtc ttacaggact
     1861 gtaacccgga cggggccgag accggacgca tcatgcaaga aatgtttgat gtgctcaata
     1921 cggccaaaga agctcgccag agcacgatgg attctgtgct tcgcgaattg gaatacatta
     1981 acggaggtct gttcaatgag catcttcctt tattgtacta ctcccgggag atgcaccaga
     2041 agcttttgga gtgtacccgt tttgactggg ggctgatctc accggttgtc tt
//
LOCUS       DAOSHE010000396         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_53044_length_2088_cov_3.857354, whole genome shotgun sequence.
ACCESSION   DAOSHE010000396 DAOSHE010000000
VERSION     DAOSHE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53044_length_2088_cov_3.857354"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLD82_09305"
     CDS             complement(<1..479)
                     /locus_tag="PLD82_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOG65616.1"
                     /translation="MTPIRLAFIGGSGIYSIDGMSIVDELAIKTPFGKPSDKIMVGAF
                     EDGSRIAFLPRHGRGHRLLPTEVNSRANIWALKSIGVESIVSLSAVGSLREEIAPRHL
                     VIPDQLIDRTRHRPESSFFGEGIVGHVSFADPYCGELRAILIKAAREAGATIHDKGTL
                     "
     gene            complement(476..757)
                     /locus_tag="PLD82_09310"
     CDS             complement(476..757)
                     /locus_tag="PLD82_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOG65617.1"
                     /translation="MRFSLEVTVHPGSKHTSIENDKGLVRVHLTAPAVEGKANAALCK
                     LLSGELGVPKTAITIQRGEHGRKKVIFVEAERLPEPYLSMCIHHPGRQP"
     gene            complement(759..1577)
                     /gene="proC"
                     /locus_tag="PLD82_09315"
     CDS             complement(759..1577)
                     /gene="proC"
                     /locus_tag="PLD82_09315"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOG65618.1"
                     /translation="MQNTLNESPRIGCIGCGNMGGALVRGIAGSGLASSITVMDVRPE
                     ASAELAAACGASAVSSIGDLAAGSDIVILAVKPGQIDTLLDANASRALKDRLVVSVAA
                     GVTLDRLSTRLPASRLIRVMPNTPALIGAGVIAWTAGSTVTEQEVLLFETVFRTAGKL
                     FRFDEKQMDAVTGLSGSGPAYVFLLLNAFAEGGAREGLNKTDALVMAAQTFLGAARMV
                     LEGGEHPEVLKDRVTSPGGTTAAGLATLEECAVRSACIKAVHAATLRSRELGGS"
     gene            complement(1558..>2088)
                     /locus_tag="PLD82_09320"
     CDS             complement(1558..>2088)
                     /locus_tag="PLD82_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG65619.1"
                     /translation="SRRSARAVPAVWHLVGHLQSNKAKKAALSFDWIHSIDSFETAGI
                     VARTASAVGRIVKILLEVNTSGEASKHGIPPEETLSLAESLAPLDGNGILISGLMTVG
                     PLGGDDTANRRAFAHLRSLRDRVQTILPGCTELSMGMSGDFASAILEGSTMVRVGSAI
                     FGTRIAPERYDAEHTE"
BASE COUNT          406 a          614 c          652 g          416 t
ORIGIN      
        1 agggttccct tgtcgtgaat ggtggcaccg gcttcacggg ccgccttgat gaggatggcc
       61 cgcaactcgc cgcaataggg atcggcaaaa ctgacatgtc cgacgattcc ctccccaaaa
      121 aagctcgatt cagggcgatg gcgggtccgg tcgatcagct ggtccgggat gacgaggtgg
      181 cgcggggcaa tctcttcacg gaggctcccg acggcagaga gggagacaat cgattcgact
      241 ccgatgctct tgagtgccca gatattcgcc cgggaattga cttccgtcgg gaggagacgg
      301 tggccacgtc catgccgcgg caggaatgcg atccggcttc cgtcctcgaa ggcgccgacc
      361 atgatcttgt ccgatggttt cccgaagggt gtcttgatgg ccagttcatc gacaatcgac
      421 attccgtcga tgctgtagat tccgctgccg ccgatgaatg cgagacgaat gggtgtcatg
      481 gctgccttcc tggatgatgt atgcacatgg aaagatatgg ctccgggagt cgttcggctt
      541 ccacgaaaat cactttcttt cggccatgct cgcctcgctg gatggtgatc gccgttttgg
      601 gaacaccgag ttcaccggag aggagcttgc agagggccgc attcgctttc ccctcgacgg
      661 ccggtgccgt caggtgcacc cggaccaggc ccttgtcgtt ctcgatgctc gtgtgcttgc
      721 ttcccgggtg aacggtgact tccagggaaa agcgcacctt acgaacctcc gagttcgcgg
      781 gaacggaggg tcgcggcgtg aaccgccttg atgcaggccg aacggacagc gcattcctcg
      841 agcgtggcca gtccggcagc cgtggtcccg ccgggcgagg tgacgcgatc cttgaggacc
      901 tcaggatgtt caccaccttc aagcaccatg cgggccgctc ccagaaaggt ctgtgccgcc
      961 atcacaaggg catcggtctt gttgagtccc tcccgggcac cgccttccgc gaaggcattc
     1021 agcaaaagga agacgtaggc cgggccgctc cccgagagac cggtcaccgc atccatctgt
     1081 ttttcatcaa atcggaagag ttttccggca gtccggaaga ccgtctcaaa gagcaggact
     1141 tcctgctccg ttacggtgct tcccgctgtc caggcgatga caccggcacc gatcaaagcc
     1201 ggggtatttg gcatgacccg gatcagtcgg gaggccggca ggcgggtgga gagtctgtcg
     1261 agtgtcactc ccgcagcgac ggagacgacg agacggtctt tgagggcacg ggatgcattg
     1321 gcgtccagaa gggtatcgat ctgccccggt ttgacggcga ggatgacaat gtcggaaccg
     1381 gccgccaggt ctccaatgga cgaaacagca cttgccccgc aggctgcggc cagttcggcg
     1441 gacgcctcgg gtctgacatc catgacagtg atcgacgagg ccagcccgga gccggcaatc
     1501 ccgcgcacga gggctccgcc catattcccg catccaatac agccgatgcg ggggctttca
     1561 ttcagtgtgt tctgcatcat atcgctccgg agcgatgcgt gttccgaaga tggcacttcc
     1621 gacacgcacc atggttgatc cttcaaggat agcacttgcg aagtctcccg acattcccat
     1681 ggaaagttcg gtacagccag ggagaatcgt ctgcacccga tccctcaggg acctgagatg
     1741 cgcaaaggcc ctgcggttgg ctgtgtcgtc cccgccaagg ggacccacgg tcatcaagcc
     1801 cgagatgaga atgccatttc cgtcaagagg cgccaggctc tcggccaggg aaagggtctc
     1861 ttcgggggga attccgtgct tggatgcctc gccggaggta ttgacttcga gaaggatttt
     1921 gacgatccgg cccacggcac tggctgttcg ggcaacgatt ccggctgttt caaagctgtc
     1981 gatggaatgg atccagtcaa aagacaatgc agcttttttc gccttgttgg actggagatg
     2041 gccgacgaga tgccagaccg cgggtaccgc cctcgctgat cttcggga
//
LOCUS       DAOSHE010000397         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_53322_length_2081_cov_3.360316, whole genome shotgun sequence.
ACCESSION   DAOSHE010000397 DAOSHE010000000
VERSION     DAOSHE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53322_length_2081_cov_3.360316"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..500
                     /locus_tag="PLD82_09325"
     CDS             24..500
                     /locus_tag="PLD82_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65620.1"
                     /translation="MKKIQTVVVALMAIMLGTGVLAAQTARRPDQGDRKKIERPQTET
                     PEMKAQRHKRHEFRVEMINLMVKHGKMEKAQADFMIKKMNNEKAFKDANPEWVKYQRR
                     HPMMRHGKPGKKGCPGMDRRPGDRRGMHEGRPGMDHRPMPRGPMMDDCGDCGDDQP"
     gene            complement(672..>2081)
                     /locus_tag="PLD82_09330"
     CDS             complement(672..>2081)
                     /locus_tag="PLD82_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG65621.1"
                     /translation="VTILHFNDFHAQVMPYKVAKNDAAPSAGLARLVHLIRSNRTADS
                     LTLCAGDILLGSEYSTVFKGEVEFSIFRGLVDFMTIGNHEFDYGISTLEALRDKNAIP
                     MLIANVSYKDARPFTRQKSAIRTINGVKVGIFGLVAENTPDETNPVNVRQLAFASEVA
                     TARTMVQELKRQGAQVIVAMTHIGFTKDKALAAAVPGIDVIIGAHSHTMLPVGERIGN
                     TLIAQANWGGSHLGKIVLEYDATSRRLVSSVASLIAATAETPEDPAARAAIDVFKGKL
                     DGKLNEVIGSTGIHLDGERSHVRARETNLGNYIADVLCDAAGAEIALMNGGGIRASIE
                     RGQIRIRDVQKVLPFNNTLQTIEISGSALMKILDRAARDAGGGGSFLQVSKDVNLVIR
                     GKRLTQVLYQGRPIQPDRIYKVATIDFLIVGGGGFVEFKEGRNIKAAGGLLSEVVIEA
                     IRKAGTIRPRSEGRIDITD"
BASE COUNT          446 a          551 c          672 g          412 t
ORIGIN      
        1 tcctgaaagg agaatgagcg aatatgaaaa agatacagac agttgtggtg gccctgatgg
       61 ccataatgtt ggggacaggt gttcttgcag cgcagacagc cagacggcct gaccagggtg
      121 accggaaaaa gatcgaacga ccccagaccg agacccccga gatgaaggca cagaggcaca
      181 agaggcacga gttccgcgtc gaaatgatca acctgatggt caagcacgga aagatggaaa
      241 aggcccaggc cgatttcatg atcaaaaaga tgaacaacga aaaggccttt aaggatgcca
      301 atcccgaatg ggtcaagtat cagcgccgcc acccgatgat gcgccatggc aaacctggaa
      361 aaaaaggctg cccaggtatg gatcgccggc ctggagaccg acgtgggatg catgagggca
      421 gacccggcat ggatcacagg ccgatgcccc gtggtcccat gatggacgat tgcggtgatt
      481 gtggggatga ccagccctga atctgacgga atcagtcatg tgtcaaaaaa ccggacggag
      541 gagatgatcc tcccgtccgg tttttttggt gtcctccccc ccggggggcg atacctgtcc
      601 tgaagaagct tttgcatggg gacgggaaac agatcgcctg tggtgcaagc tcccgggggg
      661 cgatgcggag gtcagtccgt gatgtcgatc cggccctcgc tgcggggacg gatcgtgccg
      721 gctttgcgga tggcttcgat gacaacttcg gacagaagac cgccggcggc cttgatattc
      781 cggccttcct tgaactcgac aaacccgccg ccaccgacga tcaggaaatc gatggtcgca
      841 actttgtaga tccggtcggg ctggattggc cggccttggt acaggacctg tgtgaggcgt
      901 ttgccacgga tgaccaggtt gacatctttg gaaacctgga ggaaggagcc accacccccg
      961 gcatccctgg ccgccctgtc cagaattttc atgagggcac tgccagagat ttcgatggtc
     1021 tgcaaagtgt tgttgaaagg aaggactttc tggacatcac ggatgcggat ctggccgcgt
     1081 tcaatggaag ccctgatccc gccgccgttc atcaaggcaa tttcggcgcc ggcagcatca
     1141 cagagaacgt cggcgatgta gtttcccaga ttggtctccc gtgcccggac gtgggaccgc
     1201 tctccgtcca ggtgaattcc ggtcgagccg atgacctcgt tgagctttcc gtcgagtttt
     1261 cctttgaaga cgtcaatagc ggcccgggcg gcagggtctt cgggagtctc ggccgttgcc
     1321 gcaatcaggg aggccacgct ggagaccagc cgcctggagg tcgcgtcgta ctccaggaca
     1381 atcttgccga gatgggaccc gccccagttg gcctgcgcga tcagggtgtt tccgatccgc
     1441 tctccgacgg ggagcatggt gtgggagtgc gctccgatga tgacatcgat accgggaacg
     1501 gcggccgcca gtgccttgtc cttggtgaag ccgatatggg tcatggcaac gatgacctgg
     1561 gctccctggc gcttgagctc ctgcaccatg gtgcgggcgg tggccacttc ggaggcaaag
     1621 gccagctgcc tgacattgac gggattggtc tcgtccgggg tgttctcggc aaccaggcca
     1681 aagataccca ccttgacacc gttgatggtc cggatggcgg atttttggcg tgtgaacggg
     1741 cgagcatcct tgtaggagac gttggcaatc agcatgggga tggcgttttt gtcccggagc
     1801 gcttcaaggg ttgaaatgcc gtagtcaaat tcgtgattgc cgatggtcat gaagtccacc
     1861 agcccgcgga agatggagaa ctcgacttca cccttaaaga cggtcgagta ttcggagccg
     1921 aggagtatat ccccggcaca gagggtcagg gaatcggctg tccggttgct cctgatcagg
     1981 tgaacgagtc gggccaggcc ggccgaaggg gccgcatcgt tttttgcgac cttgtagggc
     2041 atgacctggg catggaagtc attgaagtgc aggatggtga c
//
LOCUS       DAOSHE010000398         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_53730_length_2070_cov_4.432754, whole genome shotgun sequence.
ACCESSION   DAOSHE010000398 DAOSHE010000000
VERSION     DAOSHE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53730_length_2070_cov_4.432754"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLD82_09335"
     CDS             complement(<1..313)
                     /locus_tag="PLD82_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65622.1"
                     /translation="MKIRGNTLLRILDRFIGPCILLVARFLPLAREHNHPRRRILVLT
                     NSAMGDAVVLSGVLREAMKRLPNAEWHIAASPLVHDVLTLAGLHYAAADLDFARPLGS
                     LK"
     gene            complement(310..>2070)
                     /locus_tag="PLD82_09340"
     CDS             complement(310..>2070)
                     /locus_tag="PLD82_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG65623.1"
                     /translation="SDRNAALERENERYRDWLEEMVRQKTEKLQISLRQLTNLRAALD
                     RTQILVVTDRDNRIRYANNRFIQLVGLSRSKLTGRDWFGLVTDVHDDLLVMNIQGTIA
                     TGKIWKGMISGRAKDGRRWWTETAIVPVAGPDGNHAQNIIIQADTTDIIESRERIRSL
                     AYTDGLTGLPNRTRFCELILTEAGHAEEYGGFAIAMLDIDGFKSFNEAYGRDIGDAIL
                     LEFVKRTQASIGKHTLSRLGSDQFGVLLRTPTSEKDALAFAGKIQSLFIKPFMINGHE
                     YHITACNGIALFPHHGTTPEELLQNASLALDLAKGEGHCHFSVYRNRMHQLALSRISL
                     ETELRQALVNEEFELFYQPKISVSSGRLVGAEALLRWRKPGLGIVPPSEFIPYAEESG
                     LIIKIGRWVLEEACRQISVWKEKGLPECIISVNVSARQFKHPNIITYVMTALTTNQVD
                     PAFLELEITENVLMNGVGSTLETLNSLHDLGIRLSIDDFGTGYSSLAYLKDFPVQVLK
                     IDRSFISNIGRDERDTNITRGIISLARNLNLCVLAEGVETEHQLQFLRAEGCDEIQGY
                     WYSPPLPAPDFFKKYAHQSP"
BASE COUNT          409 a          555 c          635 g          471 t
ORIGIN      
        1 tctttaaact ccccagcggc cgggcaaagt cgagatccgc ggctgcatag tgcagtccgg
       61 ccagtgtcaa aacatcatgc accagagggc tggccgcaat atgccactcc gcattcggaa
      121 gacgcttcat cgcttccctc aggacaccgg agaggacgac agcgtcgccc atcgccgaat
      181 tcgtcaggac gagaatccgc cggcggggat ggttgtgctc gcgtgcaagc ggcaggaaac
      241 gcgccacaag gaggatgcat ggtccgatga atcggtcaag gattcgaaga agcgtgtttc
      301 cccggatctt catggggact ggtgtgcgta ctttttgaaa aagtctggag cgggaagcgg
      361 ggggctgtac caatacccct ggatctcgtc gcaaccttcc gcacgcaaaa actggagttg
      421 atgttctgtt tcgacaccct cggccaggac acagaggttg aggttgcgag ccaggctgat
      481 gataccccgt gtgatgtttg tatcccgttc atccctgccg atattgctga tgaaggaacg
      541 gtcgatcttg agaacctgta ccgggaagtc tttcaggtag gccagcgagg aatacccggt
      601 tccgaaatca tcaatggaaa gacggattcc gagatcatgc agggagttca gcgtttccag
      661 cgtcgagccg accccgttca tcaggacgtt ttccgtaatc tcgagctcaa ggaaggccgg
      721 gtcaacctgg tttgtggtca gcgccgtcat gacgtaggtg atgatgttgg ggtgcttgaa
      781 ctgacgggct gaaacattga ccgagatgat gcattccggc aaacccttct ctttccatac
      841 ggagatttgt cggcatgcct cttcgagaac ccatcggccg attttgatga tcagtccgga
      901 ctcttcggca taggggatga attccgaagg ggggacgatc ccgagacccg gtttgcgcca
      961 gcggagaagt gcctccgctc cgaccagtcg tcccgatgag acggaaatct ttggctggta
     1021 gaagagctcg aattcctcgt tcacgagggc ttgcctgagc tcggtttcga gggagatcct
     1081 cgagagggca agctgatgca tccggttgcg gtagacgctg aaatggcaat ggccttcacc
     1141 tttggccaga tcgagtgcca gcgacgcgtt ttgcagcagt tcctcggggg tcgtgccgtg
     1201 atggggaaag agggcgattc cgttacaggc cgtgatgtgg tactcatgtc cgttgatcat
     1261 gaatggcttg atgaagagcg actgtatctt tcccgcaaag gccagcgcgt ccttctcgct
     1321 ggtcggggtc cgcaggagga cgccaaactg gtcgctcccg aggcgtgaca gggtgtgctt
     1381 tccgatggag gcctgcgtgc gcttgacaaa ttcgaggaga attgcgtcgc cgatatcccg
     1441 gccataagct tcattgaaag acttgaagcc gtcgatgtcc agcatggcta tggcgaagcc
     1501 gccgtactct tcggcatggc cggcttccgt caggatcagc tcgcaaaaac gggtccggtt
     1561 cgggagccct gtcaagccgt cggtatatgc caatgatcgg atccgctccc ggctctcgat
     1621 gatgtcggtc gtgtcggcct ggatgatgat gttttgggca tggtttccgt ccggcccggc
     1681 tacgggaaca atggctgttt cagtccacca gcggcgaccg tctttggcac gaccgctgat
     1741 catgcccttc catatcttgc cggtggcaat ggttccctga atgttcatca cgagcaggtc
     1801 gtcgtggaca tcggtcacca atccgaacca gtcgcgcccg gtcaacttgc ttctggacaa
     1861 tccgaccagt tggatgaaac ggttgttggc gtagcggatc ctgttgtctc tgtcggtcac
     1921 gaccaggatt tgggtccggt ccagtgcggc gcggaggttg gtcagctgcc ggagggagat
     1981 ctggagcttc tcggttttct ggcgaaccat ctcctccagc cagtcccgat agcgctcgtt
     2041 ttccctttcg agtgcggcat ttctgtcgga
//
LOCUS       DAOSHE010000399         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_53765_length_2069_cov_3.967726, whole genome shotgun sequence.
ACCESSION   DAOSHE010000399 DAOSHE010000000
VERSION     DAOSHE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53765_length_2069_cov_3.967726"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLD82_09345"
     CDS             complement(<1..1683)
                     /locus_tag="PLD82_09345"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG65624.1"
                     /translation="MAEQARLTGAWVLLLLAALLTGILAGGVLWPAPLLDALLGKAAW
                     FFPLYLLALSLYAFISGSVLKSLARSLPLCLLLPTALFLLHAGDPKLAGSLGQTLARP
                     LVSLAGDSSIWLGGFILLALFVFVFVRSLLILQQNTSPIRQRQPMRSGGDRVLYPGYA
                     SDALEHVQSSQAIPAFTPGEASREQAETTPLRRSEEGYRFTTGISRRDLLKAFKKQAS
                     ESGETPPMVTILDEEAAAAWYASQTASAMNPDENTPVPEPAMEQTSHGNGSLVRDGFP
                     RETSPANTEIPVEPGPSGESSPFIEPDSTNAEIPDLQEPVSKPSDEPCDRAHPDNPPT
                     ESEPLAVYHDVAPSIMDAPREEVLRTDSAFVPRRDRSEYRKPDASLLVKSHERIDEDT
                     RAQMQATAEALEKTFFEFGIEARVTNICRGPVITQYEMSVPPGIRLSRVVALQDNIAL
                     NLAAASVRIEAPIPGKSAIGIEVPNRKRSMVTLRDILESPEFQNATARIPLALGQMVS
                     GAPMVADLAAMPHLIIAGTTGSGKSVCVNSIICSILYNMRPSQVRFILVDPKY"
     gene            complement(1864..>2069)
                     /locus_tag="PLD82_09350"
     CDS             complement(1864..>2069)
                     /locus_tag="PLD82_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65625.1"
                     /translation="LPDPIFILFGNHTASGFIPAYHISGFLGAFVVGLIALTLLVRLL
                     KAGRFHWFGWYCLAASLFWTLVH"
BASE COUNT          411 a          532 c          704 g          422 t
ORIGIN      
        1 gtatttcggg tcgaccagga taaagcggac ctggctgggg cgcatgttgt agaggatgct
       61 gcagatgatg gaattgacgc aaaccgattt tccggaaccg gttgttcctg cgatgatcag
      121 gtgtggcatg gcggccaggt ccgcgaccat gggcgccccc gagaccatct gtccgagggc
      181 cagggggatg cgggccgttg cattctggaa ctcgggcgat tccaggatgt cccgcagtgt
      241 caccatcgag cgtttccggt tcggaacttc gattcctatg gcggattttc ccggaatcgg
      301 ggcttcgatg cggacgctgg ccgcagccag attcagggcg atattgtcct gaagggcgac
      361 aacccgggag aggcggattc ccggtgggac ggacatttcg tactgggtga tgacaggtcc
      421 ccggcagata ttggtcacgc gggcttcgat cccgaattcg aagaaggttt tttcgagagc
      481 ttccgcggtg gcctgcatct gggcacgggt gtcttcgtcg atccgttcat gggatttgac
      541 aagcaggctt gcgtccggtt tccggtattc gctcctgtcg cgacgcggaa caaaggcgga
      601 gtcagtccgc aggacttctt cccggggtgc atccatgatt gagggtgcaa cgtcgtgata
      661 cacggccaag ggttctgatt ccgtcggggg attgtccggg tgcgctctgt cacaaggctc
      721 gtccgatggt ttggagaccg gttcctgaag atcggggatc tctgcattcg tgctgtctgg
      781 ttcgatgaag ggactgcttt ccccggacgg gccgggttcg acgggaattt ccgtgtttgc
      841 tggcgaggtt tcccgcggga aaccgtcccg gacgagagac ccgttgccgt gactggtctg
      901 ctccatggca ggctccggga ctggcgtgtt ttcgtccgga ttcatcgcgg atgctgtctg
      961 gctggcatac caggctgctg ccgcttcttc atcgagaatg gtcaccatcg ggggcgtttc
     1021 tccgctctcg ctggcctgct ttttgaaagc cttcagaagg tctcttcggg agatcccggt
     1081 ggtgaagcgg tagccttcct ccgaacggcg tagcggtgtg gtttccgcct gttcccgtga
     1141 tgcctctccc ggggtgaagg ccggtatggc ctgggatgac tggacgtgtt ccagcgcgtc
     1201 cgaagcatat cccggataga gcacgcgatc cccgcctgac cgcatgggtt gtcgttggcg
     1261 gatggggctg gtgttttgtt gcagtatcag gagggagcgc acgaagacaa agacgaagag
     1321 tgccaggagg atgaatccgc caagccagat cgaggaatcg ccggcgagtg agacgagtgg
     1381 gcgagccaag gtttggccaa ggctccctgc gagtttcggg tcgccggcat ggaggagaaa
     1441 gagcgccgtc ggaaggagca ggcacagagg caaggaccgc gccaagcttt tcaggaccga
     1501 acccgagatg aaggcgtaga gagagagtgc aagaaggtag agcgggaaaa accacgccgc
     1561 cttccccagg agcgcatcga ggagtggcgc tggccagaga acgccaccgg ccagaatccc
     1621 ggtgaggagc gcggcaagaa ggagaagcac ccatgcgcct gttaaccgtg cctgttctgc
     1681 cattcctgct ccagattgtt tgcggatgat tctcccggta tcgcgggagg gtatttcgag
     1741 tatcggactc ttcagtcttg ctcatgagtt gcaccatggt gtcgacaaac tggcaccatg
     1801 gtgttggttg aatcgggaaa gagcatcaag gctccccgca aaaaggcacc gtgagggcaa
     1861 aaatcagtgt accagcgtcc agaagaggga tgcggccagg cagtaccagc cgaaccagtg
     1921 aaatcggccg gccttgagga gacggacaag cagggtcagg gcaatcaggc cgacgacaaa
     1981 tgcgccgaga aagccggaga tatggtacgc ggggataaag ccggatgctg tgtgattgcc
     2041 gaaaagtata aaaatcgggt ccgggagca
//
LOCUS       DAOSHE010000400         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_54252_length_2057_cov_4.461039, whole genome shotgun sequence.
ACCESSION   DAOSHE010000400 DAOSHE010000000
VERSION     DAOSHE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54252_length_2057_cov_4.461039"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLD82_09355"
     CDS             <1..528
                     /locus_tag="PLD82_09355"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65626.1"
                     /translation="GLVSELEAADEACFETRLQEALEAMHRLWCRPEAVNPLFEAAGS
                     PPITHPAPLSQLAARGAVPLETLLAQCDTTRFPALAGGRADSEVLAAAAIELRYEGYI
                     AREREAIARMLRMEEKLIPDSFRWESVRGLSAEGRMVLTKVRPRTLGQASRLAGVRAA
                     DISIIMVHLGGGGRG"
     gene            525..1592
                     /gene="mnmA"
                     /locus_tag="PLD82_09360"
     CDS             525..1592
                     /gene="mnmA"
                     /locus_tag="PLD82_09360"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG65627.1"
                     /translation="MKVAVLVSGGVDSALALKLLVDKGHECTAFYLKIWLEDELSFLG
                     DCPWETDLGYVRATCAALGVPLEVVPLQREYHDRVVRYTLDELRAGRTPSPDIFCNER
                     IKFGAFFDHIGPEYRSVATGHYARLIRDGERTLLALAPDPVKDQTYFLAHLSAAQLER
                     TLFPVGDLHKSEVRRLAAEYGLPPAGRKDSQGICFLGKIKFRDFAKSYLGECTGKIVE
                     QGTGRVLGEHAGYWFHTIGQRTGLGLSGGPWFVVRKDIATNTIEVVHADNYQVAQVSS
                     FPVEEIHWIQQAPAAGAGLRCKLRHGPTMFACSLELDSGLPGTGRVHLLDGKDAGCAP
                     GQFAVFYLDGICLGMARIGTA"
     gene            1698..>2057
                     /locus_tag="PLD82_09365"
     CDS             1698..>2057
                     /locus_tag="PLD82_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65628.1"
                     /translation="MSGERRILLVEDDPMTAAMEEMYLQDAGYSVMTAGSGPKAVDLA
                     LSRDVDLVLMDVDLGPGMDGTEAARRILAERDIPLIFLSSHTEPEIVTRTESITSYGY
                     IVKTSNSAVLLASIRMAF"
BASE COUNT          380 a          595 c          659 g          423 t
ORIGIN      
        1 ggactggttt cggagctgga ggccgcggac gaggcctgct ttgagacccg gctgcaggaa
       61 gcactggagg ccatgcatcg cctctggtgt cgccccgagg cggtcaaccc gcttttcgag
      121 gctgccggtt caccgcccat cacacatccc gcgccgcttt cgcaactggc tgcgcggggc
      181 gcagttcccc ttgaaacact ccttgcgcag tgcgacacca cgcgttttcc ggcccttgcc
      241 ggcggacgtg cggacagcga ggttcttgcg gctgcggcca tcgaactgcg gtacgagggg
      301 tatatcgccc gcgagcggga agcgattgcc cgcatgctgc gcatggagga aaaactgatt
      361 cccgattcgt ttcgctggga gtctgttcgc gggctctcgg ccgaggggcg gatggtgctg
      421 accaaagtcc gtccgcgaac cctggggcag gcttcgcggc tggccggagt cagggctgcc
      481 gatatctcga tcatcatggt gcatctgggt ggaggggggc gagggtgaag gttgccgtgc
      541 tggtttcggg cggggtggac agtgctctgg ccctcaaact tcttgttgac aagggccacg
      601 agtgtacggc cttttatctc aagatctggc tggaggacga actttcgttt ttgggggatt
      661 gtccgtggga aacagatctg ggctatgtcc gggcgacctg tgccgccctc ggggtgcccc
      721 ttgaggtagt gcccctccag cgggagtacc acgaccgggt cgtccggtac accctcgacg
      781 agctccgggc ggggcggact cccagtccag acatcttctg caacgaacgg atcaagttcg
      841 gagctttttt tgaccatatc gggccggaat accggtccgt ggccaccgga cactatgcga
      901 gattgatccg tgatggtgag cggacactcc ttgccctggc tcccgatccg gtcaaggatc
      961 agacctattt tctggcccat ctctcggcgg cccagttgga gaggacgctc tttcctgtcg
     1021 gagatctcca caaaagcgag gtcaggcgtc tcgcggccga gtacggtctc cctccggccg
     1081 gacgcaagga cagccagggg atctgctttt tggggaagat caaattcagg gattttgcca
     1141 aaagctatct gggcgaatgc accggaaaaa ttgtcgagca gggcaccggc cgggttttgg
     1201 gcgagcatgc gggctattgg ttccacacca tcggacagcg gaccggactc ggactctccg
     1261 gtgggccatg gttcgttgtt cgcaaggata tcgcgaccaa taccatcgaa gtggtccatg
     1321 ccgacaatta tcaggtcgcc caggtcagca gctttcccgt cgaagagatc cactggatac
     1381 agcaggcccc ggccgccggt gccggtttac gctgcaagct caggcacggt ccgaccatgt
     1441 ttgcctgcag cctcgaactg gacagcggtc tccccggaac cggccgggtc cacctgctgg
     1501 acgggaaaga cgccggctgt gcccccggac aatttgccgt tttctatctg gacgggatct
     1561 gtctgggcat ggcccgcatc ggaacggcct gatccggtcg gaaaatattc cggcgtgcgc
     1621 atcccatttg aagaaaactg gcttgacaag aataccgccg gtcgtgcagg atgatgtcaa
     1681 tacgcaaggt ggtatccgtg tcgggcgaaa gacgtatcct gctggtcgaa gatgatccca
     1741 tgaccgcagc catggaggag atgtatcttc aggatgccgg atattccgtg atgacggccg
     1801 ggtccggtcc aaaggctgtc gatctggctc tctcaagaga cgtggatctt gtcctcatgg
     1861 atgtcgatct cggaccgggg atggacggga ccgaggcagc caggcggatc cttgccgaac
     1921 gcgatatacc gctcatcttc ctttcgagcc ataccgaacc ggagattgtg acacggaccg
     1981 agagcatcac atcctacggg tacatcgtca agacgagcaa tagcgcggtt ctgttggcat
     2041 ccatccggat ggccttc
//
LOCUS       DAOSHE010000401         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_54499_length_2051_cov_3.510020, whole genome shotgun sequence.
ACCESSION   DAOSHE010000401 DAOSHE010000000
VERSION     DAOSHE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54499_length_2051_cov_3.510020"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLD82_09370"
     CDS             complement(<1..258)
                     /locus_tag="PLD82_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65629.1"
                     /translation="MPTLVYPIVMDPAVPGIATISGSGAAEEDALEALELAIALELEP
                     DVSDEADCVSPVSPPVAEPSKSMGGQRKMLSERVGIVGAKAS"
     gene            200..1120
                     /locus_tag="PLD82_09375"
     CDS             200..1120
                     /locus_tag="PLD82_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG65630.1"
                     /translation="MVAIPGTAGSITIGYTSVGMTPHSVAGITGFEIGKCEITYSLWT
                     NVRDWALANGYVFSTKTVGRMGGSDLGGSFTPEHPVTEVCWPDVIVWCNALSVMRGYS
                     PVYRTDSGKLFPLTDVNMLGDAYYPTHVDWTKNGYRLPTEAEWEYAARRKTDGSLQDG
                     NKPSGYPGTAIEGDQSLPTEWGPYCWYGFISGGSTHPVGEKLANTMGLYDMSGNVSEW
                     CWDWFMGDYEETPEYTGQDPTGTSMPQDPTGTSMPGDGRSIRGGAAYSTYGSSYNQQT
                     AWRDGINGSIISVIDWPDLATIGFRVVRRP"
     gene            1432..>2051
                     /locus_tag="PLD82_09380"
     CDS             1432..>2051
                     /locus_tag="PLD82_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65631.1"
                     /translation="MSRSSQRKNRRERLARLKSRIRVQEDYIAHLERVSEAYTRLDEL
                     SREELIEADQIIRAQEIIQEMLREEKKDAVETIHAHESVHELMSEEKKMADMTIRAHE
                     QVEALAAREKKEAIETIRALHSIEELAARERQEAYSFIEAQDKVSQLSMQELVERDKA
                     LHAILKVNSRISSHLDEDLLLAEILDSMTDALSATHGSVWLSTTGG"
BASE COUNT          470 a          596 c          585 g          400 t
ORIGIN      
        1 cgatgccttt gcgcccacga tccccacgcg ctcgctcaac atcttccgct ggcctcccat
       61 cgatttcgat ggctcggcca ccggcggaga taccggagac acacagtctg cctcgtcgga
      121 aacatcgggt tcaagctcga gtgcaatggc cagctccagt gcttccagcg catcctcctc
      181 cgcggcaccg ctccccgaga tggtggcgat cccgggaaca gcggggtcca tcacaatcgg
      241 atatacaagt gtcggcatga caccccattc agtcgccggg atcacgggct tcgagatcgg
      301 caaatgcgag attacctaca gcctctggac caatgtgcgc gactgggcgc tcgccaatgg
      361 ctatgtcttt tcgaccaaaa cggtggggag aatgggggga agtgatcttg ggggatcatt
      421 cacccctgag catccagtaa ccgaagtctg ctggccggat gtcatcgtct ggtgcaatgc
      481 tctctcagtc atgcgcgggt acagtccggt ctaccgcact gattcaggaa aactctttcc
      541 cttgacggat gtgaatatgc tgggagatgc ctactatccg acacacgttg actggaccaa
      601 aaatggctac cgccttccga ccgaggccga atgggagtac gccgcccgcc gcaaaactga
      661 cgggagtctg caggacggaa acaagccatc cggatatcct gggaccgcta tcgaaggaga
      721 ccagagtctt cctacagagt ggggacccta ttgctggtac ggttttattt ccggtggatc
      781 tacccacccg gtcggcgaaa agctcgccaa cacgatggga ctctacgaca tgagcggcaa
      841 cgtgtccgaa tggtgctggg actggtttat gggggactat gaagaaactc ccgagtatac
      901 cggccaggat ccgacaggga ccagcatgcc acaggatccg acagggacca gcatgccagg
      961 tgatggaaga agcatccgcg gtggtgctgc ctattcaacc tatggcagct cctacaatca
     1021 gcagacggca tggcgtgatg gtattaacgg atcaataata agtgtgatcg attggccaga
     1081 tctcgccacc atcggcttca gggtcgtgcg ccggccctga gccggtagag aacacgacac
     1141 acgcatggag ggctgtcccg catgggcagc cctttttttc ttccagttat tccatgagca
     1201 tcatgggact cccggaatca caccactgtt tcatgatatc gatatgcgag tccgggagtc
     1261 gaatcatgca ttcgcgtttt tgtgctgacg cttgatttca ggagcaggtg gtttctcctc
     1321 gtccttgacg ggtggctccg actctggtac gctgtctcta ccggtgaaca ggcgctgttt
     1381 cgcaatggct tgaggatgtt caccaaaaaa atgctgtcca ggatgcactc catgtcacgc
     1441 agctcacaac gcaaaaatcg ccgggaacgc ctggcgcgtc tcaaaagccg gatcagggtc
     1501 caggaagact atatcgccca cctcgagcgg gtgtccgagg cctatacgcg actggacgag
     1561 ctttcccgcg aagaactgat cgaggccgac cagatcatcc gggcccagga aatcatccag
     1621 gagatgctgc gcgaagagaa aaaggatgcc gtggagacga tccacgccca cgagagcgtc
     1681 cacgagctga tgtccgaaga gaaaaagatg gccgacatga ccatccgggc acatgaacag
     1741 gtcgaggcgt tggctgcccg cgaaaaaaaa gaagccatcg agacgatccg ggccctgcac
     1801 agtatcgaag agcttgccgc ccgtgaacgg caggaagcct acagctttat tgaagcgcag
     1861 gacaaggttt cgcagctctc gatgcaggag ctggtcgaac gggacaaggc cctgcacgcc
     1921 atcctcaagg tcaacagccg gatcagctcc caccttgatg aggatcttct tctcgccgaa
     1981 atcctggact ctatgacgga tgccctctcg gccacacacg gctccgtctg gctctcgacg
     2041 accggcggct g
//
LOCUS       DAOSHE010000402         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_54562_length_2050_cov_2.234085, whole genome shotgun sequence.
ACCESSION   DAOSHE010000402 DAOSHE010000000
VERSION     DAOSHE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54562_length_2050_cov_2.234085"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..>2050)
                     /locus_tag="PLD82_09385"
     CDS             complement(69..>2050)
                     /locus_tag="PLD82_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65632.1"
                     /translation="SGIPSDHEETMARLHTHITTIHQIVSRLETARSRAEEMRPLYES
                     FFVSRLLFRRINEAYVDVSRTHAAEINTISSRSRILSLLLFAAIGCYILLLLGFRKIL
                     VNRLTLFQKKLAGATDAPELWPYEDELTPLVNTWETMRRSLSTSVEEIALLKNCLHSV
                     MDAMPSILVAFDQNGRILQWNASASHETGLDEQEALGRSIWDSFPLISTFRDDLVIAI
                     SENNNLSIRRELLRDGQYRYLAINAFPLGETHATGTVLRIDDVTDHERRETRLRQSQK
                     METIGTLAGGIAHNFNNILAGIVGTLSLMRLEEFTRDSTYLERLDTLETASQRAVDMV
                     QQILSLARRQEASMSPIDMAVSLRHILQICQNTFDRSIEIRSEIPRGPLTVMADPVIL
                     EQALLNICVNAAHAMTTMRDKGSSPGGVLSVHVENTDLTADALKSRPGKNPGRWWCVT
                     IQDTGIGIPVQIQQKIFEPFFTTKPTGQGTGLGLSTTWSIIEQHDGFIQLYSEIGKGS
                     VFRIWLPAVTATHVNGQSRPAPDLPPGHGLILVVDDEDIVRRTAQALLERCGYQVVTA
                     ENGDDAIKLHENWKTSIRAVLLDLTMPGKSGQETLLAMRANDPEVCVVISSGFHDPED
                     METLVNGGAAGFIGKPYTIAGLARGLDEAIRRSGK"
BASE COUNT          395 a          526 c          681 g          448 t
ORIGIN      
        1 gtgagaccga tgccatggtt ctttcgatga tgctcgagaa gacaggaacc ggaacaggag
       61 actgacactt attttccaga gcggcggatg gcctcgtcaa ggccacgggc cagaccggcg
      121 atggtatagg gcttcccgat aaagccggcc gcgccgccgt tcacgagtgt ttccatgtct
      181 tccgggtcat ggaagccgga actgatcaca acacagacct cgggatcgtt ggctcgcatg
      241 gccaagagcg tctcctgccc gctttttccg ggcatggtca gatccagaag gacggctcgg
      301 atgctggtct tccagttctc gtggagcttg atggcatcgt caccgttttc tgccgtgaca
      361 acctggtacc cacagcgctc gagcagggct tgggctgtcc ggcgcacaat gtcctcatca
      421 tccacgacga ggatcagccc gtgtccggga gggaggtccg gggcaggacg ggattgtccg
      481 ttgacatggg tggccgtgac ggccggaagc cagatgcgga agacgcttcc ctttccgatc
      541 tcggaataga gctggatgaa accatcatgc tgttcaatga tcgaccaggt cgtggagagc
      601 ccgagtcccg ttccctggcc ggtgggtttg gtggtgaaaa atggctcaaa gattttttgc
      661 tggatctgga ccgggatgcc gattcctgta tcctggatag tgacacacca ccagcgtccc
      721 gggttttttc cgggccggga tttcagggca tcagcagtca ggtcggtgtt ctcgacatgg
      781 accgaaagaa cgccacccgg ggatgagccc ttgtcccgca tggttgtcat cgcgtgggcg
      841 gcgttgacac agatgttgag gagggcctgc tcgaggatga cgggatcagc catgacggtg
      901 agggggccac ggggaatctc cgaacggatc tcgatggatc ggtcaaaggt gttctgacag
      961 atctggagga tgtgtctcaa cgagacggcc atatcgatcg ggctcatcga ggcttcctgg
     1021 cgtcttgcaa gggagagaat ctgctggacc atgtcaaccg cccgttgcga tgcggtctcg
     1081 agggtgtcga gccgttcgag ataggttgag tcccgggtga attcttcgag ccgcatcaag
     1141 gagagcgtcc ccacaattcc ggcaagaata ttgttgaagt tatgggcgat tccgccggcg
     1201 agtgtgccga tggtctccat tttttgggac tgccgcagcc ttgtctcgcg ccgttcgtga
     1261 tccgtaacat cgtcaatccg cagcacggtg cccgtggcgt gggtttctcc gaggggaaag
     1321 gcgttgatgg ccagataccg gtattgtccg tcccggagga gttccctccg gatggagagg
     1381 ttgttgtttt cgctgatggc gatcacgaga tcgtcgcgga aggtcgagat cagcgggaag
     1441 gagtcccaga tgctccggcc gagggcctcc tgttcatcca gtccggtctc gtgggatgcc
     1501 gaggcattcc actgcaggat ccgtccgttc tggtcaaagg ccacgaggat tgaaggcatg
     1561 gcatccatga cgctgtgcag gcagttcttg agcagggcga tttcttcaac cgatgtcgag
     1621 aggctgcggc gcatcgtctc ccatgtgttg accagggggg tgagctcgtc ctcgtagggc
     1681 cagagttccg gggcatccgt cgcgcccgcc agcttttttt ggaagagtgt cagtctgttg
     1741 acgagaattt tccggaaacc gaggaggagc aggatgtagc atccgatggc agcaaagagc
     1801 aggagcgaga ggatgcggct tcgggacgag atcgtgttga tttcggcagc atgcgtccgg
     1861 gagacatcca cgtaggcttc gttgattctc cggaagagga gccggctgac aaaaaaggat
     1921 tcatagagcg gtcgcatctc ttctgcccgg gagcgcgccg tttcgagacg ggaaacgatc
     1981 tgatggattg tggtgatatg ggtgtggaga cgggccatgg tctcctcgtg atccgagggg
     2041 attccggatg
//
LOCUS       DAOSHE010000403         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_54585_length_2049_cov_3.378134, whole genome shotgun sequence.
ACCESSION   DAOSHE010000403 DAOSHE010000000
VERSION     DAOSHE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54585_length_2049_cov_3.378134"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLD82_09390"
     CDS             <1..1479
                     /locus_tag="PLD82_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG65633.1"
                     /translation="QYALLKLLAGGHNNACIVGDDDQSIYSWRGARVDNFAAFTRDFT
                     GVKAIRLEQNYRSTPQILEIAAKVIEKNDERMAKTVWSALPEGDKPRVISFQRDRDEA
                     EWIAGELLDLQRKGYSLEDCAIFYRANWQSRIFEEALLSKNIPSQLVGSMRFYERREI
                     RDLVAWLRLAVNPSDGSAFARAMQAPLRGVGEKSIASLVAYAEAHGMPVVQVCSQHAA
                     SLVRGKAKQEALGKIGNLVKNFAESADPLSKRVARLAKDSGLMEFHSEEEERIENIQE
                     FINSISDWEDHNPKGTAAEYLAEVSLLGQTDETSGGGKGVFLMTVHNAKGLEFDTVFT
                     TGLEEGIFPHYLSIDDPARLQEERRLFYVAATRAKKRLFLCHAASRYSFGREMNNEPS
                     RFLDEFPPELVDWEEVDPKRSTSSAHWKNRLSEEKRTRPEHTNRPQTFSQGEKVRHRE
                     YGPGVILERSGEGKLVRLRVAFTDRVCTFIELYSGLESEEKR"
     gene            1689..1979
                     /gene="gatC"
                     /locus_tag="PLD82_09395"
     CDS             1689..1979
                     /gene="gatC"
                     /locus_tag="PLD82_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG65634.1"
                     /translation="MAVDRTTVERVARLSRLELSEPEKEALTTQMARIVQFVEKINEL
                     DTSGIEPLPRDATSANVEREDVPHKNAVSRDEILALAPESRDGSILVPRVIE"
BASE COUNT          499 a          581 c          555 g          414 t
ORIGIN      
        1 cagtatgccc ttctcaagct cttggccgga ggacacaata acgcctgcat cgtgggcgac
       61 gacgaccaat ccatctattc atggcgtgga gcccgcgtgg acaattttgc ggcgtttacc
      121 cgtgatttta caggggtcaa ggccatcagg cttgaacaga attaccgctc gaccccgcag
      181 atcctcgaaa ttgcagccaa agttatcgaa aagaacgatg agagaatggc aaagaccgtc
      241 tggagcgccc tcccggaagg cgacaaaccc agggttatct cttttcaacg cgacagggac
      301 gaagccgaat ggatcgccgg cgaactcctg gatctccaac ggaaagggta ttcactcgag
      361 gactgcgcga tcttctatcg ggccaattgg caatcccgca tcttcgagga agctctgctt
      421 tccaaaaaca ttcccagcca gttggttggt tccatgcggt tttatgaacg ccgtgaaatc
      481 cgtgatctcg tcgcctggct ccggctcgcg gtcaacccga gcgacgggag tgcctttgcc
      541 cgcgccatgc aagcgcccct acgcggagtc ggtgaaaaga gcattgccag tctggtcgcc
      601 tatgccgaag cacacggaat gcctgttgta caagtctgtt cacagcatgc ggccagcctt
      661 gtcaggggca aagccaaaca ggaagccctc ggcaagatcg ggaacctggt caagaatttc
      721 gcggagtcgg cggatcccct cagtaaacgc gtagcccgac tggccaagga ttccggtctc
      781 atggaattcc attccgaaga ggaagaacgg atcgagaaca tccaggaatt catcaactcc
      841 atttccgact gggaggatca caatcccaag ggaacagcag ccgagtatct tgcagaagtc
      901 tctctccttg gacaaaccga tgaaaccagt ggaggaggga agggcgtctt cctcatgaca
      961 gtacacaatg ccaaggggct cgaattcgac acggtgttca caacgggact ggaagaaggg
     1021 atttttcccc actatctctc cattgacgat cctgccaggc ttcaggagga acgtcggctc
     1081 ttttatgtcg ccgcgacccg cgccaagaaa cggcttttct tgtgccatgc cgcctcacga
     1141 tacagcttcg gacgcgaaat gaacaacgaa cccagccgtt ttttggacga gtttcccccc
     1201 gaactggttg actgggaaga ggtggatccg aaacggagca ccagttccgc tcactggaaa
     1261 aaccggcttt ccgaagagaa acggacacga ccggaacaca caaaccggcc acagaccttc
     1321 tcccagggcg aaaaggtccg gcatcgtgag tacgggcccg gtgtgattct cgaacgatcc
     1381 ggagaaggaa aactggtccg cctccgtgtc gcctttaccg accgggtctg cacctttatc
     1441 gagctctact cgggtctcga atccgaagag aagcggtaaa gagccccgga agaggcatcg
     1501 ttgtgcaacc gtccccctcg attctcgcgg attggcatga cagaccggga ttgactttgc
     1561 gacaccatga tatttttttt acagagtatc tcgtatcaga tggcacggtc tcccggagca
     1621 acagcgtctg gtcacaaaga cagggtattt cgggaaacac gatgcaggat ccgcgcaagg
     1681 agttgtccat ggctgtcgat cgtaccacgg tggagcgtgt cgcacgcctc tcccggcttg
     1741 aactgagcga accggaaaag gaagccttga ccacccagat ggccaggatt gtccagtttg
     1801 tcgaaaaaat caacgagttg gatacatccg gtatcgaacc tcttccccgt gacgccacca
     1861 gcgccaatgt cgagcgagag gatgttcctc ataaaaatgc cgtgtcgaga gacgagattc
     1921 tcgctctggc cccggaatcc cgggatggca gcatcctcgt tccccgagtc atcgaataac
     1981 tcccgtaagg atctctctga tgaatcttgc cagcttgaat attgcccgac ttcgcaaggg
     2041 actggatgc
//
LOCUS       DAOSHE010000404         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_55041_length_2038_cov_3.638427, whole genome shotgun sequence.
ACCESSION   DAOSHE010000404 DAOSHE010000000
VERSION     DAOSHE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55041_length_2038_cov_3.638427"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLD82_09400"
     CDS             <1..431
                     /locus_tag="PLD82_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG65635.1"
                     /translation="ERTIEGNWNAGLQLSIPISELLNPESKSWNGSAQHGHSIKKAQL
                     LRSNVESLIQLEVKQNFLRLEENRKTIASQKVAVELSTEGLRTARIAYNEGQIGNVQL
                     MDAELDYQKAQLMEYQSWFGYISAKIDLYKAMGILTATLQ"
     gene            470..1522
                     /locus_tag="PLD82_09405"
     CDS             470..1522
                     /locus_tag="PLD82_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG65636.1"
                     /translation="MKLKFRLTKKQLVIAGVGLLVALIAFSRAKKDTGADTGPQLVPV
                     VIEDVTNTTVVQRLLINGEIKGLNQADVYPDVPGKILELYVSEGQYVGAGQIVATIDR
                     NQVGMIFMPATVRAPISGIVGKIYVEKGRTIAPSVPVMLIADTRIVEGVLNVPEKDIG
                     LYKIGQEAEIRIESQPGMVFKGYVSRITSLIDPLTRTLEVRLTLVNTDNKLIPGNYAD
                     FAIKVKEIPNQVVVPFDAVIDMFERKEVYVVENAESPHGGAAVKDNPGTPKTVPLGSG
                     KYLVARQRQIVTGIRDGGMIQIVEGLKPGERVISFGKENVIDGTLVRPVKLATRAAAQ
                     TTGTTNAATNAAAGQR"
     gene            1529..>2038
                     /locus_tag="PLD82_09410"
     CDS             1529..>2038
                     /locus_tag="PLD82_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG65637.1"
                     /translation="MKLIDTSIKRPVFTVMIYVAILILGFTGLSKLSIDFLPEIEFPA
                     IVILTQQPGVGPQEIESSITRVIEGAVASAEGIDRLTAVSREGLSAVTVLFKWGKNLD
                     AAVADLRDKIDLVIDRLPDDASKPTLFRISTSNIPIMFLGLVGEKPLPFLFELADQKL
                     KDQIVQVPGI"
BASE COUNT          480 a          540 c          591 g          427 t
ORIGIN      
        1 acgaacggac gatcgagggg aactggaatg ccggactcca gctctcgatt ccgatctccg
       61 aactcctcaa cccggagtcc aagtcctgga acgggtcggc ccagcacggg cattccatca
      121 aaaaggccca attgttgcgc agcaatgtcg agtccctgat ccagctcgag gtcaagcaga
      181 acttcctccg gcttgaggaa aaccgcaaga cgattgccag ccaaaaggtg gctgtggaac
      241 tctcgacgga ggggctgcgc acggcccgca tcgcctacaa tgaggggcag atcggaaacg
      301 tccagctcat ggatgccgaa ctggattatc aaaaagcaca actgatggag taccagtcct
      361 ggtttggcta catctccgcc aagattgatc tctacaaggc aatgggtatt ttgacggcga
      421 cactgcagta aacaaagcgc agtatgatga aaggataagg agaggtatca tgaagctcaa
      481 gttccgcttg accaaaaaac agttggtgat tgccggcgtg gggcttttgg tcgccctgat
      541 cgccttcagt cgggcgaaaa aagacacagg tgcggatacc gggccccagt tggttcccgt
      601 tgttatcgag gatgtgacaa ataccacagt ggtacagcga ctgctcatca acggggagat
      661 caaagggctc aatcaggcag atgtctaccc ggatgtaccc ggcaagatcc tcgaactcta
      721 tgtttcagag gggcagtatg tcggggccgg gcagatcgtc gcgacgatcg acaggaatca
      781 ggtcgggatg atctttatgc cggccacggt ccgggccccg atttctggta ttgttggcaa
      841 gatctatgtc gagaagggac gtacaatcgc tcccagcgtg ccggtgatgt tgatagccga
      901 cacccggatt gtggaagggg tcctgaatgt gcccgaaaag gacatcggac tctacaagat
      961 tgggcaagaa gccgagatcc gtatcgagag tcagcccggg atggttttta agggctatgt
     1021 ctcgcgcatc acgtcgctga tcgatcccct gacacggacg ctcgaggtcc gtttgacact
     1081 ggtcaatacc gacaacaagc tgattcccgg caactatgcc gattttgcca tcaaggtcaa
     1141 ggagattccc aatcaggtgg ttgtgccctt tgatgcggtc atcgacatgt tcgagcgcaa
     1201 ggaagtctat gttgtcgaaa atgcggagtc cccccatggc ggcgcggccg tcaaggacaa
     1261 cccggggacg ccaaagacgg taccccttgg atccggcaaa tatctcgtcg cccggcagcg
     1321 gcagatcgtg accgggatcc gggacggggg catgatccag attgtcgagg ggctcaagcc
     1381 gggagaacgt gtcatctctt tcgggaagga aaatgtcatt gacggaaccc ttgtccggcc
     1441 agtcaagctg gccacacggg cagcggcaca gaccacaggg acgacaaatg cggcaaccaa
     1501 tgccgcggcc ggtcagaggt aagcgcccat gaagctgatt gacacatcca tcaagagacc
     1561 tgtttttacg gtgatgatct atgttgcgat cctcatcctc gggtttaccg gtctttcgaa
     1621 actctccatc gattttttgc cggagatcga gtttccggct atcgttatcc tgacacagca
     1681 acccggtgtc ggtccgcagg agatcgagtc ttctattacc cgcgtgatcg agggggccgt
     1741 tgcctctgcc gaggggattg accgtttgac cgccgtttcg cgtgagggat tgtcggctgt
     1801 caccgtcctg ttcaaatggg gaaagaacct ggatgcggcg gtagcggatc tgcgggacaa
     1861 gatcgacctt gtgatcgatc gtcttcccga tgacgcatca aaacccacac tcttcaggat
     1921 aagcacatcc aacataccaa tcatgtttct tggtctggtg ggcgaaaagc cgcttccctt
     1981 tttgtttgaa ctggctgacc aaaagctcaa ggaccagatc gtccaggtcc ccgggatt
//
LOCUS       DAOSHE010000405         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_55116_length_2036_cov_3.583039, whole genome shotgun sequence.
ACCESSION   DAOSHE010000405 DAOSHE010000000
VERSION     DAOSHE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55116_length_2036_cov_3.583039"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLD82_09415"
     CDS             <1..1048
                     /locus_tag="PLD82_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG65638.1"
                     /translation="EQARLETERILNGPQLAKEPLAGRMQQRASTVDTVYGGLLSGRQ
                     EAVKILETMVSGFIDDTGPNQDASLLLKDIISQEQRFLANHCLNVAVVSLAAAIELNK
                     MMTRKLDDPGIQKDINLLRAIRLKTFTREELVKLGFAAFVHDIYYKKVFPHLKHTDVL
                     NALADRSNIERHASESYHLMRQFEMDYHVNKAVLQHHERIDGTGTPDGISERLFSKYT
                     AILSFADRYITLTQPNPFSPLFHPTVALKRIMTTEKSGFDLDAMVAFAHASTMVPIGS
                     WVLLENGLIGYSSRREEGQNLPIIHPVMKSSGDPIENPEDVAPRYDGNRIRQLLTAGD
                     LMAINPKYASLYLA"
     gene            complement(1153..>2036)
                     /locus_tag="PLD82_09420"
     CDS             complement(1153..>2036)
                     /locus_tag="PLD82_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65639.1"
                     /translation="KWFTMQMSPRANERDAPADEKYQLIGFPLPGEGRVSFEYALPEI
                     TWTPMLTASILDTKTVSLRVQAAITMGANQAFEKCSLKLVLNNAMSLQSLSGQTFNLD
                     SFDLFPYRNVMYSLENRNVDYRFIREWNTYAGGDTVYIKLLVKNPFSINLSQTRYTVD
                     YRQVNIDYGSLGTAARPGDTLTLDAGTDNSIKTFRAVKITENRDNKHLPFNHYIAYTI
                     QNTSDEEKIVYLVSSRITGNEHRSVYHFRKPPDATPENTLIWILTLAPHGTQTLEYDY
                     DADIKDVPGENGFEQGG"
BASE COUNT          455 a          583 c          542 g          456 t
ORIGIN      
        1 cgaacaggcc cgtctcgaga cggaacgcat tttgaacggc ccgcaactcg ccaaggaacc
       61 acttgccgga cggatgcagc agcgtgccag tacggttgat accgtctacg ggggcttatt
      121 aagcggtcgt caggaagccg tcaagatcct cgaaacaatg gtctcgggct ttatcgatga
      181 taccggaccg aaccaggatg cctccctcct cctcaaggac atcatcagtc aggaacagcg
      241 ctttctggcc aatcactgcc tgaatgtcgc agtcgtctcc ttggcagcag cgattgagct
      301 caataaaatg atgacgcgca agcttgatga ccccggcatc caaaaggaca tcaatctcct
      361 gcgggccatc cgactcaaga catttacccg cgaggaactt gtcaagctgg gctttgccgc
      421 cttcgtccac gatatctact ataaaaaggt ctttccccat ctcaagcaca ccgatgtgct
      481 caatgccctc gctgaccgca gcaatatcga gcgccacgcc tccgagagct accaccttat
      541 gcgccaattc gagatggact accatgtcaa taaagcggtt ctccagcatc atgaacggat
      601 cgatggaaca ggcactccgg acgggatttc cgaacgcctc ttttcgaagt acacggcgat
      661 tctctccttc gcggaccgct atatcacact cacccaaccc aacccctttt caccgctctt
      721 ccatccgact gtcgccctca aacgcatcat gacaaccgaa aaatccggct ttgatctgga
      781 cgccatggtt gcctttgccc acgcgtcgac catggtcccc atcggctcct gggttcttct
      841 cgaaaacggc ctgatcgggt attccagccg gcgggaagag ggccaaaacc tgcccatcat
      901 ccatccggtc atgaagtcca gcggggatcc aatcgaaaat cccgaggacg tcgccccccg
      961 ctatgacgga aaccggatcc gccagctcct gaccgccggt gatctgatgg ccataaaccc
     1021 caagtatgcc agtctctatc tggcctgaac ccgtgtcgag ggcggatacg ctgttttgat
     1081 gaaacgtatt tcgaatcggc cagaacagaa tctccatcct gaccgatcca gtacggacgc
     1141 accgtaccct gatcagccac cctgctcgaa gccgttttca ccagggacat ccttgatgtc
     1201 ggcatcgtag tcgtactcca gagtctgggt tccgtggggc gccagtgtga ggatccagat
     1261 gagcgtgttt tcgggagtcg catccggtgg cttgcggaag tgatacaccg acctgtgctc
     1321 atttcctgtg atccggcttg aaacaaggta cacgatcttt tcttcatcgg aagtgttctg
     1381 gatcgtatag gcaatgtaat ggttgaaagg cagatgcttg ttgtcgcgat tttcggtgat
     1441 cttgaccgcg cggaaggtct tgatcgagtt gtcagtcccg gcgtcaagag tgagagtatc
     1501 ccctggtcgg gcagcggtcc cgagtgaccc gtaatcgata ttgacctgcc tgtagtcgac
     1561 tgtataccgg gtctgcgaga gattgatcga aaaggggttt ttgacgagaa gcttgatata
     1621 gacggtatcc ccgccggcat aggtattcca ttcgcggatg aatctgtagt cgacattgcg
     1681 gttttcgagg ctgtacatca cgtttcgata gggaaagagg tcaaacgagt caagattgaa
     1741 tgtctggccg gagaggctct gcaggctcat ggcattgttg agcacaagtt tgaggctgca
     1801 cttttcgaag gcctggtttg cccccatggt gatcgccgcc tggacacgga gggataccgt
     1861 cttggtatcc aggatggatg cagtcaacat cggggtccag gtgatttcgg gcagggcata
     1921 ctcgaacgaa acccggccct cacccggcag ggggaacccg atcaactggt atttttcgtc
     1981 ggcaggcgca tcgcgctcat tggcccgggg cgacatctgc atggtaaacc acttga
//
LOCUS       DAOSHE010000406         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_55398_length_2029_cov_3.476190, whole genome shotgun sequence.
ACCESSION   DAOSHE010000406 DAOSHE010000000
VERSION     DAOSHE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55398_length_2029_cov_3.476190"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLD82_09425"
     CDS             <1..436
                     /locus_tag="PLD82_09425"
                     /inference="COORDINATES: protein motif:HMM:NF025027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HOG65640.1"
                     /translation="SIDELRLRKLEKIVADLQKKFEENNLQSILDNVIKDPKLTFNPG
                     RQILERIIKELYRIHNPDSKKLEKTECLEEMITDHQFSKKIDRKIFTYIRYVKEIGNT
                     GSHHQDNATPDTAAALHMLEQLCQIIEWYLSHRDGFATPREK"
     gene            516..1373
                     /locus_tag="PLD82_09430"
     CDS             516..1373
                     /locus_tag="PLD82_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65641.1"
                     /translation="MTGLRMFIILLLLALATVVSGANPPTNLDFLSSDPAMIAGLKAP
                     NHDLALKEQLVLLNNNGGIPFYNMTTRRYQRIRQPANDQGNPMPQIAGAEQHFMANRD
                     VVLLQLAPGKRFLVFSHSASPVTGATTIWEVGPRYPSLKLAFSGELDAVRRRANGQIV
                     LVFGDITGKVEVVYNPVGNTLTPAFALSLHEMQHGSPIYPDGSVKYSTPKSVRIRAAS
                     GTPLYAEPNNTAQVLARTPLQSAWALASLGDWRLVIIPVRVNDWWLNVFAGKTWRPVW
                     IEAFKILEQ"
     gene            1425..>2029
                     /locus_tag="PLD82_09435"
     CDS             1425..>2029
                     /locus_tag="PLD82_09435"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG65642.1"
                     /translation="MNTRLIVYSLVFSFSLGQTLLSAPSVLTGIDVLEASGFSVLRGR
                     KIGLITNHTGKNARGERTVDVLWRASRRSGFSLVRLFSPEHGLSGTADDRVLSGKDAA
                     TGLKVISLFNKNYAPQPADLVGLDTLVFDIQDIGTRFYTYHGTMSLAMRAAARAGLRF
                     IVLDRPNPIGGVLVQGAIPPPEKCGSLVTIHPIPTRHGMTIG"
BASE COUNT          474 a          562 c          536 g          457 t
ORIGIN      
        1 cagtattgat gaattgcggc tgcgcaagct cgaaaagatt gttgcggatt tgcagaagaa
       61 attcgaagaa aacaatctgc agagcatatt ggataatgtg atcaaggacc caaaattgac
      121 attcaaccca gggcgacaaa tactggaaag gattatcaag gaactctaca gaatccacaa
      181 cccggattca aaaaagttgg aaaagactga atgccttgaa gaaatgatca ctgaccacca
      241 gttcagcaaa aaaatcgaca gaaagatctt tacctatatc cggtatgtca aggagatcgg
      301 gaataccggt tcacaccatc aggacaatgc gacgccagat actgctgcgg ccttgcacat
      361 gctcgaacaa ctctgccaga tcatcgaatg gtacttgtcc caccgtgacg ggtttgccac
      421 accccgcgaa aaatgattcc cgcttgctga caaaaccacg tcccgggtgt atcctcctcc
      481 atacctgcga attctcgccg gcgaggagga agactatgac gggcttgagg atgttcatca
      541 tactgctgtt gctggcactg gcaacggttg tgtcaggggc gaacccgccc accaatctgg
      601 atttcctgtc cagtgatccg gccatgattg ctggcttgaa agcgccaaat catgatcttg
      661 cgctgaaaga acagctcgtg ttgctcaaca acaacggtgg cataccgttt tacaatatga
      721 caacaaggcg ttatcaacgg atacggcaac cggccaatga tcagggcaac ccgatgccgc
      781 agattgccgg tgccgaacag cattttatgg ccaaccggga tgtcgtcctc ctccagcttg
      841 cacccggcaa gcgctttctc gtcttcagtc attccgcgag tccggttacc ggtgccacga
      901 ccatctggga agtcggaccg cgctatcctt ccctcaaact ggctttttca ggcgaactcg
      961 atgctgtacg acgcagggcc aacgggcaga tcgtcctcgt gttcggtgat atcaccggga
     1021 aggtcgaagt cgtctacaat ccagtgggga atacactgac cccggcgttt gctctctcgt
     1081 tgcatgagat gcaacatggg agtccgatct atcccgacgg ttcggtcaaa tacagtaccc
     1141 caaaatccgt gcggatcagg gctgcgtccg gcacgcctct ctatgcggaa cccaacaaca
     1201 cagcacaagt ccttgcccgc acaccgctcc agtcagcctg ggcactggcg tcgctggggg
     1261 actggcggtt ggtgatcatt cctgtccgtg tcaacgactg gtggctgaat gtctttgcag
     1321 gaaaaacatg gcggcctgtc tggatcgagg ccttcaaaat tctcgagcaa tgatcctcaa
     1381 gatccggtct tggacaggcg ggtgttcatg ccgataatca aggcatgaat acccgcctga
     1441 ttgtatactc acttgttttc agtttttctc ttgggcagac gctgctttcg gcacccagtg
     1501 tcctgacggg gatcgatgtt ctcgaggcgt ccggtttttc ggttctccgg gggcggaaga
     1561 tcggcctgat cacaaaccat accggaaaaa atgcgcgtgg cgagcggact gtcgatgtct
     1621 tgtggcgcgc gtccaggcgc tccggttttt cgcttgtgcg cctgttttca cccgagcacg
     1681 gactctcggg gacagcggat gaccgcgttc tctcggggaa ggatgcggcg acaggtctca
     1741 aggtcatcag tctcttcaat aaaaattatg cgccccagcc ggccgatctc gtcggtcttg
     1801 atacactcgt ttttgacata caggatattg gtacacggtt ttatacctac catggaacaa
     1861 tgtccctcgc catgcgcgcg gcggcccggg ccgggttgcg ctttatcgtg ctggacagac
     1921 ccaacccgat cggtggtgtg cttgtccagg gggcgatacc acctcccgaa aaatgtggca
     1981 gccttgtaac catccatccg atcccgaccc gccacgggat gaccattgg
//
LOCUS       DAOSHE010000407         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_55410_length_2029_cov_2.733029, whole genome shotgun sequence.
ACCESSION   DAOSHE010000407 DAOSHE010000000
VERSION     DAOSHE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55410_length_2029_cov_2.733029"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLD82_09440"
     CDS             <1..619
                     /locus_tag="PLD82_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65643.1"
                     /translation="QVDSVAASAGLSAGLYTAGGTGRVRVVRSSSTGWIFAGVSGTAG
                     IFGGFTTVPSASRLASAAPVSDCTADGTLLAFERSDASIATLTPELDLAAPARLWGLS
                     GGLEQRPRLFPAPDGSLYLTAQAQSVGANDMFIVKLDPGMTLRSVKPGVHDFGSEWTP
                     ESTTGPLFASDTGEASTPAEFGITGAAVSWNVQSAVSLAVHYWYR"
     gene            complement(826..>2029)
                     /locus_tag="PLD82_09445"
     CDS             complement(826..>2029)
                     /locus_tag="PLD82_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG65644.1"
                     /translation="LGINQPQVFLHANTLNTILQRSFIRTMPKAVEIAVVLLLPVFFG
                     YVSRRLRPSRIILAGTAFLAAFAFVNQLLFGAGIWMQMAPQSFALLATFFIITGIHYV
                     QGDKEKKYIKNAFGHYLSPDVIDVLVKDPAALKLGGERKVLTAYFSDVQGFSTISENL
                     SPEALVDLLNAYLTDMTDIIFHQKGTVDKFEGDAIIAFFGAPVPFEDHALRACLASLE
                     MQARVAEMRPLWKKKYGHELFVRMGINTGPMVVGNMGSRNRFDYTMMGDSVNLAARLE
                     GVNKYYGTYTMASEFTVKDLGDAIVTRELDVLRVVGKSEPVTVYELVARKGELSAEKA
                     EVLERFAEGLVAYRKKNWAAAQKHFKAAMNLDPADGPSRVYHERCAEFRKKAPPAGWD
                     GVYTLKGK"
BASE COUNT          418 a          626 c          586 g          399 t
ORIGIN      
        1 ccaggttgac agcgttgctg catccgcagg gctatccgca gggctgtaca cggcaggcgg
       61 cacgggccgg gtacgggtag tccgctccag cagcacaggc tggatttttg cgggggtcag
      121 cggcacagcg ggcatcttcg gaggattcac aacggttccc tccgcaagca ggctcgcaag
      181 cgctgcgccc gtcagcgact gcacagcgga tggcacgctc ctggcctttg agcggtctga
      241 tgcctcgatt gcaactctta cccccgaact cgaccttgct gccccggccc gtctctgggg
      301 actgtccggc ggacttgaac aacgccccag gctcttcccc gctcccgacg gcagcctcta
      361 ccttacggcc caggcgcaat ctgtgggggc caacgatatg ttcatcgtca agcttgatcc
      421 cgggatgacc ctccgctccg tcaagccggg tgtgcatgat ttcggaagtg aatggacacc
      481 ggagagcaca accggacccc tcttcgccag cgatacgggg gaagcaagca cgcctgccga
      541 attcggcatc acgggtgcgg cagtctcgtg gaacgtgcag tccgccgtct cgcttgcggt
      601 gcactactgg tacagatagc gcccaggctc aaaacaacat gactacaggt caggccggcg
      661 cgcaatgcgc cggctttttg cggcccggta ctccgggagt accgcgcacg atattgcact
      721 atcctgatgc gactccggga gtcacatgac aaaaacgcaa tacgatacca tctgcacgcc
      781 cggcgcacac agcacgcccg gacacacgct cccccgtacg ccccctcatt ttcccttgag
      841 ggtgtagacc ccgtcccagc cggccggcgg agcctttttg cggaactcgg cacagcgctc
      901 atgatagacc cgggacggac cgtccgccgg atcaagattc atggctgcct tgaaatgctt
      961 ttgggctgcc gcccagtttt ttttgcgata ggccacaagc ccttcggcaa accgctcaag
     1021 gacttctgcc ttctccgctg agagctctcc cttgcgggcc accagctcgt agaccgtcac
     1081 cggctcgctc ttgccgacca cccgcaggac atccagttcg cgggtgacaa tcgcgtcgcc
     1141 aagatccttg accgtaaatt cggaggccat ggtatacgtc ccataatact tgttcacgcc
     1201 ttccagccgg gctgcgaggt tcaccgaatc gcccatcatc gtatagtcga acctgttccg
     1261 gctgcccatg tttcccacga ccatcggtcc ggtattgatg cccatccgga caaagagttc
     1321 gtgtccgtac ttttttttcc agagcgggcg catctcggcc acccgggcct gcatctcgag
     1381 cgaggccagg caggctctca gtgcgtgatc ttcgaacggg acgggagcac cgaagaaggc
     1441 gatgatggcg tcaccctcga atttgtcgac ggttcccttt tggtggaaaa tgatgtccgt
     1501 catatcggtc agataggcat tcaaaaggtc aacgagagct tcaggcgaga gattttccga
     1561 aatggtcgaa aagccctgga catcggaaaa ataggccgtc aggaccttgc gctcgccacc
     1621 gagcttgagc gcagcgggat ccttgacgag gacatcgatc acgtcgggcg agagatagtg
     1681 cccaaacgca tttttgatgt actttttttc tttgtcaccc tggacgtagt gtatccccgt
     1741 gatgatgaag aaggtcgcca ggagagcaaa ggactgtgga gccatctgca tccagattcc
     1801 cgccccgaag agcaactggt tgacaaaggc aaacgccgct aaaaacgccg ttcccgccag
     1861 aatgatccgt gagggccgca ggcggcgcga gacgtaccca aagaaaacag gcaggagaag
     1921 cacaaccgcg atttcaaccg ctttgggcat ggtcctgatg aagctgcgct gcaggatggt
     1981 attgagggta ttggcgtgga gaaagacctg gggctggttg atcccgagg
//
LOCUS       DAOSHE010000408         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_55615_length_2024_cov_3.388522, whole genome shotgun sequence.
ACCESSION   DAOSHE010000408 DAOSHE010000000
VERSION     DAOSHE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55615_length_2024_cov_3.388522"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /gene="murF"
                     /locus_tag="PLD82_09450"
     CDS             complement(<1..1251)
                     /gene="murF"
                     /locus_tag="PLD82_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOG65645.1"
                     /translation="MRAIPFGILAAGMQARVLAVPESAGPSGVSIDSREGQSGTIFFA
                     IRGERFDGHQYLGEVLATGAAAVVAEEEAFAGLPARDQLLERAAGNGQGVYLVRDSRQ
                     ALGDAARAALDFWKPRVIAVTGSSGKTTTRRILQTLLDETWKIRTGVKNFNNEIGLPL
                     TVFTLEDGDEILLLEMGMNHAGELKRLADIARPEIVVITNIGTAHIEHLGSRDGIAAA
                     KKEALAFCTGESMAVLNSEDPYFDFLSTGVPGSIVPFGAVPEGFTLVRDNGLDGYVLG
                     YAGGETVFALGGAHNLLDLSAGVALAHKLGLATSVLANRISLVRAVESRSQVVDGRVR
                     VINDCYNANPESMRAGLNLLAAASGGRRVAVLADMLELGGDSARLHGELGEWIAASQG
                     VDELVLLGPGMAECGRSALNAGFEP"
     gene            complement(1248..>2024)
                     /gene="murE"
                     /locus_tag="PLD82_09455"
     CDS             complement(1248..>2024)
                     /gene="murE"
                     /locus_tag="PLD82_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HOG65646.1"
                     /translation="CCNSSIQFGERVHPRIASSGISVRYFGVSSESVAGQPVPDVAVI
                     DETASLERTSFVLKTSDAGIPVSLEARGFFNVYNSLAAACAALAVGIPLGQIPGGLSR
                     VRIPGRFELVENTRGILVAVDYAHTDDALSNLIRSARRLEPARVITVFGCGGDRDRKK
                     RPLMGHAASSLSDLVIVTSDNPRSEDPDAIIRDIMPGIIDPEKAIIEPDRRRAIFRAV
                     SLARRGDIVLIAGKGHEDYQIFRDRTIHFDDREVAREALS"
BASE COUNT          445 a          654 c          560 g          365 t
ORIGIN      
        1 gggctcaaac ccggcattca aggctgaacg cccgcactcc gccattcccg ggccaaggag
       61 caccagctcg tcgaccccct gactggcggc aatccattct ccgagctcac catgaagacg
      121 tgccgaatcc cccccgagtt cgagcatgtc ggcaagcacg gcaacgcgac gtccccctga
      181 agctgccgcg aggaggttca acccagcccg catcgattcc ggattcgcgt tgtaacagtc
      241 gttgataacc cgtaccctcc cgtcgacgac ctgcgaacgg gattcgaccg cacgcaccag
      301 actgatgcga ttggcaagaa cgcttgtggc aaggcccagc ttgtgtgcca gggcaactcc
      361 cgcggaaaga tcgagaaggt tatgcgcccc tcccagagca aataccgtct ctccccccgc
      421 atacccgagc acatatccat caagaccatt gtccctcacc agggtaaagc cctcgggtac
      481 agcaccgaag ggaacgatac tccctggaac tccggtcgat aaaaaatcaa aatacggatc
      541 ttcagaattg aggacggcca tggattcccc ggtacaaaac gccagcgcct cttttttggc
      601 ggcggcgatg ccgtcccggc tgcccagatg ctcgatgtgg gccgtcccga tattggtgat
      661 gacaacaatc tccggtcggg ctatatcggc cagcctcttc aattctccgg catggttcat
      721 ccccatctcg agcaagagga tctcgtcgcc atcctcgagt gtgaagactg tcaagggcag
      781 accgatctcg ttgttgaaat ttttgactcc tgtccggatc ttccaggtct cgtccagaag
      841 ggtctggagg atgcggcgtg ttgtcgtctt cccgctcgat cccgtgactg cgatcacccg
      901 cggtttccag aaatcgagag cagcacgggc cgcatcgccc aaggcctgtc tggaatcccg
      961 aaccaggtag accccctgcc cgttgcccgc ggcccgttcg agaagctgat cacgcgccgg
     1021 gaggccggca aatgcttcct cctcggccac aaccgccgcg gcaccggtgg ccaggacttc
     1081 gccgagatac tgatgcccgt caaagcgctc accccggatc gcgaagaata tcgttccgct
     1141 ctggccttcc cgggaatcga tactcacccc ggacggaccg gccgactccg gcacggcaag
     1201 gacacgggcc tgcatcccgg ccgcaagaat cccgaaggga atggctctca tgagagggcc
     1261 tcccgcgcga cttcacggtc atcaaaatgg atcgtccggt ccctgaagat ctggtaatcc
     1321 tcatgtccct tgcccgcgat caggacgata tccccgcgtc gggccagact gacagccctg
     1381 aagatggccc gtctccggtc cggttcgatg atcgcctttt cgggatcaat gattcccggc
     1441 atgatgtcac ggatgatggc atccggatcc tcgctgcgcg gattgtcact cgttacgata
     1501 acgagatccg agagggatga agccgcatgt cccatcaggg gacgcttttt cctgtcacga
     1561 tccccgccac agccaaagac cgtgatcacc cgtgccggtt cgagccggcg ggctgaccgg
     1621 atcagattgg aaagggcatc atcggtatgg gcataatcga ctgcgaccaa aattccacgg
     1681 gtgttttcga ccagctcaaa ccgtccggga atccgcaccc ggctcaagcc ccccggaatt
     1741 tgcccgaggg gaatgccgac tgccaaagcg gcacaggctg ccgccaggga attgtacaca
     1801 ttgaagaaac cgcgggcttc aagggaaaca ggaatgccgg catcggaagt cttgagaacg
     1861 aaggatgtcc tctccagact ggccgtctcg tcaatgaccg cgacatcggg aacaggctga
     1921 ccggccacgg attcactgga cacaccgaaa tagcggaccg aaataccgga agaagcgatc
     1981 cggggatgga cacgctcacc aaactggatg gacgagttgc aaca
//
LOCUS       DAOSHE010000409         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_56438_length_2004_cov_4.458697, whole genome shotgun sequence.
ACCESSION   DAOSHE010000409 DAOSHE010000000
VERSION     DAOSHE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56438_length_2004_cov_4.458697"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLD82_09460"
     CDS             <1..630
                     /locus_tag="PLD82_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG65647.1"
                     /translation="NGWQVIGDTAYPGYMEIPQYIILGYSTIFREMENSINKPGKSDV
                     DFVFLPAGVGGVASAGTSYYALRYGKNRPKIVCVEPKESDCFLESIKNGGGDPLFTKG
                     NQMSIMAGLNCGMPSLIAWPIMRDATELFVGVPDDWAETAMRAYYREGVVSGESGAAA
                     LAGLIALMTTDEMKAAREKIGINSGSKVFVLNTEGDTDPVNWKKVVGKS"
     gene            692..>2004
                     /locus_tag="PLD82_09465"
     CDS             692..>2004
                     /locus_tag="PLD82_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG65648.1"
                     /translation="MRFWCNGTEVVYEGDLSRTLLSWLRNDAGLTSAKDGCSGQAACG
                     ACMVEIDGKPVLSCVTRLEKLEGAHVTTIEGWPESVRRTIGRAFVDAGAVQCGFCTPG
                     FLARTKILLQNNPRPTRDEIIEALSKNVCRCTGYVKIIEAVEMAARALAAGREIELYE
                     PGKVGTRQPKYDAYYKAIGKSPFVDDLRLEGMLFGALRMSDHPRARVLSINTSRAESM
                     PGVLRVVTARDVPGQRHVGLVEQDWPVYIAVGEITRYIGDVLACVVAGTEEQARAAAA
                     VIDIEYEVLPPLVAMTEAEVSQIRVHPDRPNLAGHCAIVRGGNAQAALESSAFVSRGV
                     YETQRIEHAFLETECAIGEPWDEGGIRVFSQSQGVYEDRKQIAALLGLPESRVDIALV
                     PAGGGFGGKEDLTVQGHAALAAWLLQRPVKVRLTRPESITMHPKR"
BASE COUNT          385 a          546 c          642 g          431 t
ORIGIN      
        1 aatggctggc aggtcatcgg ggataccgcc tatccgggat acatggagat accgcagtac
       61 atcattcttg gctattcaac gatctttcgc gagatggaga actccatcaa caagccgggt
      121 aagtctgacg tcgattttgt ttttcttccc gcgggtgttg gtggtgtggc ttcggcgggt
      181 acgagttact acgccctgcg ctacggaaaa aaccgcccga agatcgtctg cgtcgagccc
      241 aaggaatccg actgttttct cgagtcgatc aagaacggag gcggggatcc cctctttaca
      301 aaagggaacc agatgtccat catggcgggc ttgaactgcg ggatgccctc cctgatcgcc
      361 tggcccatca tgcgtgatgc gaccgaactc tttgtcggtg tcccggatga ttgggccgag
      421 accgccatgc gggcctatta tcgggaaggc gttgtctctg gtgagtccgg agccgcggcc
      481 cttgccggac tgattgccct gatgacgacc gacgagatga aggcagcccg cgagaaaatc
      541 gggatcaact ccggttcaaa ggtctttgtg ttgaacaccg agggtgatac cgacccggtc
      601 aactggaaga aggttgtcgg aaagtcctga agcggttttt cagcggaact ttttttgggg
      661 aacagagggt cggtgaataa aggggtgaag gatgcgtttt tggtgtaacg gtaccgaggt
      721 ggtctatgag ggtgaccttt cgaggacgct tctttcctgg ttgcgcaatg atgccggtct
      781 gacttcagcc aaagacgggt gttcgggaca ggcggcctgc ggtgcctgca tggtggagat
      841 cgatgggaaa cctgtcctgt cctgtgtgac caggctggag aaactcgaag gcgcacatgt
      901 gacgacgatc gagggctggc cggaatccgt ccggcgcacg attggccggg cctttgtgga
      961 tgccggggcc gttcagtgcg gcttctgcac tcccgggttt ctcgccagga caaaaatcct
     1021 tctccagaac aatccccggc cgacacggga cgagatcatc gaggcccttt caaaaaacgt
     1081 ttgccgctgc accgggtacg tcaagatcat cgaggctgtc gagatggcgg cccgggccct
     1141 ggccgccggc cgagaaatcg agctgtatga gccgggcaag gtcgggaccc ggcagcccaa
     1201 gtatgacgcc tattacaagg cgattggcaa aagccccttt gtggacgacc tccgcctcga
     1261 ggggatgctt tttggcgccc tgcgcatgag tgatcatccg cgtgcccggg ttctttccat
     1321 aaacacgtcg cgtgcggaat ccatgcccgg tgtcttgcgg gtcgttacgg cccgggatgt
     1381 tcccggtcag cgccatgtcg ggcttgtgga gcaggattgg ccggtctata ttgcggtcgg
     1441 tgaaatcaca cgctacatcg gggacgtttt ggcctgtgtt gtggccggga ccgaggagca
     1501 ggcccgtgcg gccgctgctg tcatcgatat cgaatatgaa gttttgcccc ccctcgttgc
     1561 aatgacagag gctgaagtca gccagatccg ggtccatccg gaccggccca atctggccgg
     1621 gcattgtgcg atcgttcgtg gcggtaacgc ccaagccgct ctcgagagtt cagcctttgt
     1681 ttcacggggc gtctatgaga cccagcgcat cgaacacgct tttttggaaa ccgagtgcgc
     1741 gattggcgaa ccctgggatg aaggcggaat ccgcgtcttt tcccagagtc agggcgtcta
     1801 tgaagacagg aagcagatcg cggcacttct cggcctgccg gagagccgcg ttgacattgc
     1861 ccttgttccg gcgggtggcg gttttggcgg caaggaagac ctgacggtcc agggacatgc
     1921 ggctctcgcc gcctggctgc tgcagcgacc ggtcaaggtg cggttgaccc gtcccgagtc
     1981 gatcaccatg catcccaaac gcca
//
LOCUS       DAOSHE010000410         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_56480_length_2003_cov_3.578542, whole genome shotgun sequence.
ACCESSION   DAOSHE010000410 DAOSHE010000000
VERSION     DAOSHE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56480_length_2003_cov_3.578542"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLD82_09470"
     CDS             <1..584
                     /locus_tag="PLD82_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65649.1"
                     /translation="RVWAASRSEGVLYILKPDGGHVVIADGFENPCALAAFEDEIYCA
                     ELSGRISIVQADGRKELLAEIEPSEISIHLAMSRTGLLYASSTQRIGENAIWNISRNG
                     TVKEFVRALMEFHGMGFDATNALYVAVTERGSGGIDMYHVGSTERQRVVSGQDIVGMA
                     FDAHNDMYIATFNKMYLVHNRHLMMAWNAVRKK"
     gene            complement(588..1319)
                     /locus_tag="PLD82_09475"
     CDS             complement(588..1319)
                     /locus_tag="PLD82_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65650.1"
                     /translation="MRGQTRILICDSDHVSGSALSQELESHGYRTLVTSNPRAVPVLA
                     REHMPGAIILVLPEPVPLSHEIKMSPQLYAIPLLVIAKENSVTEELSFLANEVLPATP
                     ETRLVVEALERYLAREKPTSETNPKSETRESWIEVRSHSAYLFEYRGVINDEKISNLR
                     NRIAELLAVGRNTFTVNLTGAGHLDNLSTSAFVSLRQMVLKSAGEIRFVLPVSSLATS
                     LTGQGVPVDEYLVDGLHGSSTETKN"
     gene            complement(1323..>2003)
                     /locus_tag="PLD82_09480"
     CDS             complement(1323..>2003)
                     /locus_tag="PLD82_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG65651.1"
                     /translation="RIGEMAAQEMAASITFVTANRLIKAHFFKKYAAILNSATLLIPQ
                     GRGITWVARRFAEPLTTFFSPADVSMNVLRGAIEHKQTLFFLGSRPDTILQAVTTLRK
                     SFPEMRIVGSYHGHFKPERGEDIVSAIRKSAPSFLFVGMGFPKQENWIIQNQKAMPST
                     IAISVGNTFDLCAGITTRGPLWLRRRGLEGLSKAFRNPLRMGRFFWIIVLVFTVFWHK
                     IFKKHRRK"
BASE COUNT          457 a          491 c          612 g          443 t
ORIGIN      
        1 agcgggtttg ggcggccagc cggtctgaag gtgtgttgta catcctcaag ccggatggtg
       61 ggcatgtcgt gattgccgat ggtttcgaga acccctgtgc gttggccgca ttcgaagacg
      121 agatatactg tgccgagctc tccggacgga tttctattgt gcaggctgat ggacgcaagg
      181 aactccttgc agagatcgaa ccgagcgaga tatccatcca tcttgcaatg tcacgaaccg
      241 gacttctcta cgcgagcagt acacagcgaa tcggagaaaa cgccatctgg aacatctcca
      301 ggaatggaac tgtcaaagaa ttcgtgcggg ccctgatgga attccacggg atgggatttg
      361 acgcgaccaa tgcgctctat gttgccgtca ccgaaagggg aagcggtggc atcgacatgt
      421 accatgtcgg atccaccgag cgccagagag tggtgagcgg acaggacatt gtgggtatgg
      481 cctttgacgc ccataacgac atgtacattg cgacattcaa caagatgtat ctggtgcaca
      541 atcgtcatct gatgatggca tggaatgccg tccgaaaaaa atgagaatca gttttttgtt
      601 tcggtactgc ttccatgaag accgtcaacc aggtattcgt cgaccggaac gccctggccg
      661 gtaagtgatg tcgccaggga tgagaccggg aggacgaagc ggatctcgcc ggccgatttc
      721 aggaccattt gcctcagtga gacaaaagcg gaggtcgaga gattgtccag gtgcccggcc
      781 ccggtcagat tgactgtaaa agtgttccgg ccgacagcaa gcaattccgc aatccggttt
      841 ctcagattgc ttatcttctc gtcgttgatc acacccctgt actcgaagag atacgcggag
      901 tgtgaccgga cttcaatcca gctctcacgg gtctcgcttt tcggattcgt ttccgatgtc
      961 ggcttttcac gggcaagata gcgttcgagc gcttcaacca cgagtcgggt ttcgggagtg
     1021 gcggggagaa cctcgttggc gagaaacgag agttcttcag ttacagaatt ctctttcgct
     1081 atgacgagaa ggggaatggc gtagagttgg ggactcatct tgatttcgtg ggagagcggg
     1141 actggttccg gcaggacaag gatgatagcg cccggcatgt gttcacgagc caggactggc
     1201 acggcacggg gattggatgt gacaagggtc cggtatccgt gagactccag ttcttgggac
     1261 agggctgatc ctgaaacatg atcactgtca cagatcagga tgcgtgtctg tccgcgcacg
     1321 gtttacttcc tccggtgctt tttgaagatt ttatgccaga ataccgtaaa tacaaggaca
     1381 ataatccaga aaaagcggcc catccggagc gggttgcgaa aggccttgga gagaccctcg
     1441 agtccgcggc ggcggagcca cagtggtcca cgcgttgtga tcccggcgca gaggtcaaag
     1501 gtgttcccga cggagatggc tatcgtggac ggcatcgctt tctggttctg aatgatccag
     1561 ttttcctgtt ttggaaaccc catgcctacg aagaggaagg acggggccga tttccgtatg
     1621 gccgagacga tatcttcacc ccgttccggt ttgaaatgtc cgtgatacga gccgacaatc
     1681 cgcatctcgg gaaagctctt gcggagggtc gtgacggcct ggagaatggt gtccggcctg
     1741 gaaccgagga aaaacaaggt ttgtttgtgt tcgatggcac cccgcaggac attcatcgaa
     1801 acatcagcgg gcgagaagaa ggtcgtgagt ggttccgcaa agcggcgggc gacccaggtg
     1861 atgccacggc cttgagggat caagagtgtc gccgagttga ggatggccgc atacttcttg
     1921 aaaaaatgcg ccttgatcag acggttggca gtaacaaagg tgatgctcgc ggccatctcc
     1981 tgagcggcca tttcgccgat ccg
//
LOCUS       DAOSHE010000411         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57040_length_1991_cov_2.917355, whole genome shotgun sequence.
ACCESSION   DAOSHE010000411 DAOSHE010000000
VERSION     DAOSHE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57040_length_1991_cov_2.917355"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLD82_09485"
     CDS             <1..979
                     /locus_tag="PLD82_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HOG65652.1"
                     /translation="LETNPASMNWKTGSIDLSGILAAGLPSDPDLRRCTRPRTHALET
                     DLDRAIQACAAPAIENATRVECRLPVKNSDRTVGALLSGQIALKHGDAGLPAQTIRIH
                     FTGSAGQSFGAFLVRGIEFTLEGEANDYLGKGLSGGIITVIPPAESGFRPEENTIAGN
                     TLLYGATSGQVFIRGMVGERFAVRNSGATAVVEGVGDHGCEYMTGGTVVVLGKTGRNF
                     AAGMSGGIAYVLDTNQLFDTLCNLDMVDIEPVTTPSDIDRLKELISEHAARTGSRLAN
                     KILAEWRETLPRFVKVMPQDYKKALQTQLQDEHKDTESVSVTEEVFKHG"
     gene            972..>1991
                     /locus_tag="PLD82_09490"
     CDS             972..>1991
                     /locus_tag="PLD82_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOG65653.1"
                     /translation="MGKPTGFMEYTREVWAEEPVSERIKHFREFHKDLPYTSLRTQAA
                     RCMDCGVPWCHSHGCPLGNLIPDWNEMVWEDRLPEALELLHHTNNFPEFTGRVCPAPC
                     EKACTLSLNNDPVSIKLIECEIIDRGFAAGLVVPRPPRVETGKRVAVIGAGPAGLAAA
                     QQLRRAGHSVTVFERDEEPGGLLRFGIPDFKLEKRLIRRRVDQMEAEGIVFECGVDAG
                     EDISANYLRRHFDAILLACGSKTARDLDVPGRNLPGVHLAMEFLAQNNRLVSGLPVDS
                     EKRISAQGKHVVVIGGGDTGSDCVGTSIRQGAAHVLQIEVLPKPRRHDGTNNPSWPDW
                     PNILRT"
BASE COUNT          446 a          616 c          556 g          373 t
ORIGIN      
        1 tctcgaaacc aaccctgcat ccatgaactg gaaaaccggc agcatcgacc tctcggggat
       61 tctcgcagcc ggtctaccct ccgatccgga tctgcgtcgc tgcacacgcc cgcggacaca
      121 tgctttggaa acggatcttg atcgcgccat ccaggcatgt gccgctcccg cgattgaaaa
      181 cgctacccgg gttgaatgca gacttccggt aaaaaactcg gaccggactg tgggcgcact
      241 tctctccgga cagatcgccc tgaaacacgg ggacgccgga ctcccggcac aaaccatccg
      301 catccatttt acaggatcgg ctggccagag tttcggggcc tttcttgtcc ggggcatcga
      361 gttcaccctc gaaggcgagg ccaacgacta tcttggcaag ggcctctcgg gcggtatcat
      421 cactgtcatt ccgcccgccg aatccggttt ccgccccgaa gaaaatacca tcgccggcaa
      481 caccctgctc tatggtgcaa catccgggca agtcttcatc cggggcatgg tcggtgaacg
      541 ctttgccgtg cgcaacagcg gagcaaccgc cgttgttgaa ggcgtcggtg atcatggatg
      601 cgagtacatg accgggggaa cagtggtcgt ccttggcaaa accggccgga attttgccgc
      661 cgggatgagc ggtggaatcg cctacgtcct ggacacaaac cagctcttcg acacgctctg
      721 caatcttgac atggttgata tcgaaccggt cacgaccccc tccgacatcg accgtctcaa
      781 ggaattgatc agcgaacacg ccgcccggac cgggagtcgc ctggccaaca agatcctcgc
      841 ggagtggcgt gaaaccttgc ctcgttttgt caaagtcatg ccgcaagact ataaaaaagc
      901 gctccagaca cagttgcagg acgagcataa agacacagaa tccgtgtccg taacggagga
      961 ggtattcaag catgggtaag ccaacaggtt ttatggaata cacacgggaa gtctgggccg
     1021 aagagccagt ctccgaacgc atcaagcatt tcagggaatt ccacaaggat ctcccgtaca
     1081 ccagcctccg gacgcaagca gcccgctgca tggactgcgg tgtcccatgg tgccactccc
     1141 acggctgtcc cctgggaaat ctgatccctg actggaacga gatggtctgg gaagaccggc
     1201 ttccggaagc actggaactc ctgcatcaca ccaataattt tccggaattt accggacggg
     1261 tctgtcctgc gccttgcgag aaggcctgca cactctcgct gaacaatgat ccggtttcga
     1321 tcaaactgat tgaatgcgaa atcatcgaca ggggcttcgc agccggcctg gtcgtcccca
     1381 gaccccccag agttgagacc ggaaagcggg tcgcagtgat cggcgccggc ccagccggtc
     1441 tggccgcagc acagcaactg agacgggcag gacactcggt caccgtattt gaacgcgatg
     1501 aggagccggg cggactgcta cggttcggca tccccgattt caagctcgaa aagcgactga
     1561 tccgccggcg ggttgaccag atggaagccg aaggaatagt ctttgaatgc ggtgtcgatg
     1621 cgggggagga catctctgcc aactatctgc gcagacattt tgacgccatc ctcctggcct
     1681 gcggctccaa aaccgcccgc gacctcgatg tgcccggacg gaacctgcca ggcgtccacc
     1741 tggcgatgga atttctcgca caaaacaacc gtctcgtttc cggcctgcct gtcgattcgg
     1801 agaagcgcat ctccgcgcag ggcaaacatg ttgttgtgat cggcggtggc gataccggat
     1861 cggactgcgt cgggacctca atccgccaag gcgctgccca tgttctccag atcgaagtcc
     1921 ttccaaaacc acggcgacat gacgggacaa acaatccctc ctggccggat tggcccaaca
     1981 tcctgcggac a
//
LOCUS       DAOSHE010000412         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57136_length_1989_cov_2.540848, whole genome shotgun sequence.
ACCESSION   DAOSHE010000412 DAOSHE010000000
VERSION     DAOSHE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57136_length_1989_cov_2.540848"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLD82_09495"
     CDS             complement(<1..1059)
                     /locus_tag="PLD82_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65654.1"
                     /translation="MSLRPARIRAWCHSVLGAVLLAGSLSCMAPVLPDQDGPYRDSFP
                     VDELWVVNGLSETIGLCNLQRLEADVLPWNANPLFLTGRVPNRIVIDGTNGLVVNSSG
                     NSITVFALRQPGRQREYRLPTGSNPWDARFIHRPGRSLIAVTSFLRNSLFILDAATGE
                     SLRELLFPEGTRPQGMALSGSDLWIALSGWNFSTGEYDPGRIIVLETADPDPLLWTIR
                     HILAVRPNPQEILPDLAGQIHVLSTGRYGKNEGCLQVFDAAAGLLKKEILTGGSPRSL
                     ALDPVNGNLHLAGGDLYRCYATGTLEPVALPTISDAVTGKDIAALAIAADRRMLFAAD
                     FANDRILALSLDDAGVLRE"
     gene            complement(1056..>1989)
                     /locus_tag="PLD82_09500"
     CDS             complement(1056..>1989)
                     /locus_tag="PLD82_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG65655.1"
                     /translation="ELPCTDEITARLTIEDRILVLDAVVAGGTDTTGRHLRHTLGVSA
                     DGEYSDQALTISAAMRLEATGGYAPRFSPSLGASLLLANGVRIFCNAGTAWKIPSLDD
                     LFWPATAFAAGNDRLRPESSWTIDSGVRLVPLEGLQFRVTGFLQHVVDMIHWQPCAGG
                     VWRPANIGEVLAHGLETVVEWAYPVNPVWNLHMQAEYSLQVNRVRVFDSTDGSQIPRT
                     PHEKASASVSLRHRTMGMFRFAAGYTGFRYLNMANTKYLGDHLLVDTSLEIRIGEHWK
                     IRATVENIFDTVAVHLREYPLPGREWSFSLEVQL"
BASE COUNT          430 a          584 c          605 g          370 t
ORIGIN      
        1 ctcccgcagg actcccgcgt cgtcgaggga gagggcgaga atcctgtcgt tggcaaaatc
       61 ggccgcaaag agcatgcgcc gatccgcagc aattgccaga gcggcaatat cctttccggt
      121 gaccgcatcg ctgattgtgg ggagcgccac cggctccagt gtcccggtcg cgtagcaccg
      181 gtacagatcg cctccggcca gatggagatt tccattgacg ggatcaagcg ccagactgcg
      241 cggactcccc ccggtcagaa tctctttttt gagcaggccg gcagccgcgt caaagacctg
      301 gagacagcct tcgttttttc cgtagcgccc ggttgaaaga acatggatct gcccggccag
      361 atcgggcagg atttcctggg gattgggccg aaccgcgagg atgtgccgga ttgtccacag
      421 cagtggatcc ggatccgcgg tttcgaggac gatgatccgg ccggggtcat actcaccggt
      481 cgagaagttc cagccggaga gggcaatcca gaggtctgat cccgaaagcg ccataccctg
      541 tggccgtgtt ccttccggga acagcagttc acgcagcgac tctcctgtcg cagcgtccag
      601 gataaagaga gaattccgca aaaacgaggt gacagcgatc aggctgcgac caggacgatg
      661 gatgaaacgg gcgtcccagg gattgcttcc ggtcgggagc cggtactcgc gctgccgccc
      721 aggttggcga agagcgaaaa cagtgatcga atttccgctg gagttgacga ccagaccgtt
      781 tgttccgtcg atcacgatcc tgttgggaac ccggccggtc agaaacaggg gattcgcatt
      841 ccagggcaag acatccgcct ccagacgctg gagattgcag agcccgatgg tttccgaaag
      901 accattcaca acccagagtt catcaacggg aaagctgtcc cggtacggac catcctggtc
      961 gggcaggacg ggagccatac aggagagact gcctgccagc aggacggctc ccaggaccga
     1021 gtggcaccat gcgcgaatgc gcgctggacg aagactcaca attgtacctc cagagagaag
     1081 gaccattccc gaccgggaag gggatattcc cgcaaatgaa ccgcgaccgt atcgaaaatg
     1141 ttttccaccg tggcgcggat cttccagtgc tctccaatcc gtatctccag cgatgtgtca
     1201 accagcagat gatcacccag gtatttggta ttggccatgt tcaggtagcg aaatccggtg
     1261 tacccggccg caaaccggaa catccccatc gttctgtgcc gcaggctgac gctggcagac
     1321 gccttttcgt ggggggttct gggtatctgt gatccgtctg tactgtcgaa gacccggaca
     1381 cgattgacct ggagactgta ctcggcttgc atgtgcagat tccagaccgg gttgaccggg
     1441 taagcccatt caaccaccgt ctcaagaccg tgcgcaagga cctccccgat attggccggt
     1501 ctccagacac caccggcgca gggttgccag tgaatcatgt caacaacatg ctggagaaaa
     1561 ccggtcaccc ggaactggag accttcaagt ggcacaaggc gcacgccaga atcgatcgtc
     1621 caggaggatt caggtctgag ccggtcgttt cccgcggcaa aggccgttgc cggccagaag
     1681 aggtcatcaa gggaagggat cttccaggcc gttccggcat tgcagaagat ccgtacgcca
     1741 tttgccagaa gcaggctcgc tccgagggag ggggagaatc ttggcgcata ccctccggtt
     1801 gcttccagcc tcatggcggc actgatcgtc agcgcttgat cgctgtactc tccgtccgcc
     1861 gaaactccga gcgtatggcg gagatgtcgg ccggttgtat cggtaccacc ggccacgacg
     1921 gcatcgagga caagaatccg gtcctcgatg gtcagcctgg cagtgatctc gtccgtgcag
     1981 ggaagctcg
//
LOCUS       DAOSHE010000413         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57222_length_1987_cov_2.580745, whole genome shotgun sequence.
ACCESSION   DAOSHE010000413 DAOSHE010000000
VERSION     DAOSHE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57222_length_1987_cov_2.580745"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLD82_09505"
     CDS             <1..1326
                     /locus_tag="PLD82_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOG65656.1"
                     /translation="DTPGHQDFVEDTFRTLTVVDSVIVVVDSAKGVEAQTEKLMEVCR
                     RRHTPVIVFINKLDRDGLDPFDVLDELESKLSITVSPMTWPAGMGHAFRGVWNRHTNR
                     FCIFSPHGKQEEGDVVVVDDVRSPVLGQRLGHAAEKLVEDLDMVSGVYPGFDRERWLK
                     GEIAPVFFGSAINNFGVKELLDCFVDIAPPPGPVAAEEREVHPQEQKFTGFVFKIHAN
                     MDPRHRDRLAFFRIVSGSFQRNEAYYHVRLDRQLRFSSPHAFLADKKEIVDEAFPGDI
                     VGLPDSGNFKLGDTLTAGEVLHYRGVPSFAPEHFRYVDNADPMRAKQLSKGLAELLDE
                     GVAQLFTSLTSGRKIIGTVGVLQLEVLQYRLEHEYNAKCRYQPIEMHKACWVSSEDPA
                     VLAEFRAKREASCALDRHGRLVFLAHSAWAIKLAQDDFPRVQFHYSSEF"
     gene            1343..>1987
                     /locus_tag="PLD82_09510"
     CDS             1343..>1987
                     /locus_tag="PLD82_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65657.1"
                     /translation="MQGYVSGLRSALTVLYGEQSSGRLMDGLVEAAVRHPFAVPPGWA
                     GRLGGPGSPLWKQCVPDIRELDECGENASSDPLAETRYAVLPGAIQRYRDRLLVFVSD
                     RCAGYCRFCTRRRMVGRNGESRSRAPSLDGMENVPQKAVTVPAERSAVVAWLRAHPEV
                     RDVLLSGGDPLMLDDDGLEAWVREIREARPDILIRIGTRMPSFAPERVSQGLCRR"
BASE COUNT          344 a          579 c          656 g          408 t
ORIGIN      
        1 gatactccgg gccaccagga cttcgtcgag gataccttcc gcaccttgac ggtggtggac
       61 agcgtgatcg tggtggtgga ttccgccaag ggtgtcgagg cccagacgga aaagctgatg
      121 gaggtctgcc ggagacggca caccccggtc atcgtcttca tcaacaagct cgaccgggac
      181 ggtttggatc cctttgatgt cctggacgaa ctggaaagca aactctcgat cacggtctct
      241 ccgatgacct ggccggcggg gatggggcat gccttccgcg gggtctggaa ccggcacacc
      301 aaccggttct gcattttcag cccgcatggc aagcaggaag agggcgatgt ggtggttgtg
      361 gacgatgtcc gctcacccgt gcttggccag cgtctcgggc atgccgccga aaaactggtc
      421 gaggatctgg acatggtgtc cggcgtctat cccggctttg accgcgaacg ctggttgaag
      481 ggcgagatcg ccccggtgtt tttcggaagt gcgatcaaca atttcggggt caaagagctc
      541 ctggactgtt tcgtggatat cgcaccaccc cccggtccgg ttgcggccga ggaacgagag
      601 gtgcacccgc aggagcagaa attcacagga ttcgttttca agatccacgc caatatggat
      661 ccccgccacc gcgaccggtt ggcatttttt cggatcgtct ccggatcctt ccagcggaac
      721 gaagcctatt accatgtccg gcttgaccgg cagctccgct tctccagtcc gcacgctttt
      781 ctggcggaca agaaggagat cgtggacgag gccttccccg gggacatcgt cggcctgcct
      841 gacagcggga atttcaagtt gggggatacc ctgacagcgg gagaggtcct gcactacagg
      901 ggtgtcccga gttttgcgcc cgaacacttc cgctacgtgg acaatgccga cccgatgcgg
      961 gccaagcagc tttcgaaggg cctggccgaa cttcttgatg agggtgtggc ccagcttttt
     1021 accagtctga cgagcgggcg gaagattatc gggacggtgg gcgtgctcca gctcgaggtc
     1081 ctgcaatacc ggctcgagca cgaatacaat gccaaatgcc ggtatcagcc gatcgagatg
     1141 cacaaggctt gctgggtcag ttccgaggat ccggcggtgc ttgccgagtt ccgcgccaag
     1201 cgcgaggcat cctgcgccct cgaccgccac ggccggctgg tttttctggc gcactctgcc
     1261 tgggccatca agctggccca ggatgatttt ccccgtgtcc agttccatta cagttcggaa
     1321 ttttaggccg gcggggatgg gtgtgcaggg ttacgtttca ggtctgcggt ctgcgctgac
     1381 tgttttgtat ggggaacagt cctcaggccg gttgatggat ggtctggtcg aggcagccgt
     1441 gcgccatccc tttgccgtcc cgccgggttg ggccgggcgg ctcggggggc cgggttcgcc
     1501 gctctggaaa cagtgtgtcc cggacatcag ggaactcgac gagtgcgggg agaacgcttc
     1561 gagcgatccc ctcgccgaaa cgaggtacgc cgtgctgccc ggtgccatcc agcgctatcg
     1621 ggacagactc ctcgtgtttg tcagtgaccg ctgcgcaggc tattgccgct tttgtacgcg
     1681 ccggcggatg gtcggtcgga atggggaatc gcgcagccgg gctccgtccc ttgacggcat
     1741 ggagaatgtt cctcaaaaag ctgtcaccgt tcctgcggaa cgcagtgctg tggtggcctg
     1801 gttgcgggca caccccgaag tacgggacgt tcttctctcc ggcggcgatc ccctcatgct
     1861 ggatgatgac ggactcgagg cctgggtccg ggagatccgc gaggccaggc cggatatcct
     1921 gatccggatc gggacgcgga tgccatcctt tgcgccggag cgggtcagtc agggcctctg
     1981 ccggcgg
//
LOCUS       DAOSHE010000414         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57294_length_1985_cov_4.021762, whole genome shotgun sequence.
ACCESSION   DAOSHE010000414 DAOSHE010000000
VERSION     DAOSHE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57294_length_1985_cov_4.021762"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..397
                     /locus_tag="PLD82_09515"
     CDS             2..397
                     /locus_tag="PLD82_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOG65658.1"
                     /translation="MARQFAARPAVHPRETGLSHGPAKSHVPHEGPSFGELLEQASST
                     TGAELKFSKHAQKRLASRNIDFSAGDMEKLQQAIDVARKKGARDSLVLMGDLALVVSV
                     KNNTVITAVDEGSTKENVFTNIDSAVIAR"
     gene            493..1863
                     /gene="flgE"
                     /locus_tag="PLD82_09520"
     CDS             493..1863
                     /gene="flgE"
                     /locus_tag="PLD82_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOG65659.1"
                     /translation="MMRSLYAGVSGLKNHQVRMDVLGNNISNVNTNGFKKGRVTFQDM
                     LSQTLSGAARPGDDKGGVNPQQVGLGMTIASIDTIHTNGSVQVTGKNTDLAILGEGFF
                     INKKGNETFYTRAGNFNIDRDGMLVNPANGYKVQGWQAQRGASGEFMVNTSTTTGDLR
                     IPVGEKDAAKATSMVDFKCNLNSLTPVVNPNAELSPDFVFKNSHSAEALVYDNYGQAH
                     KMTVNFTRQGVNIWRAQFTVADGSNVVTDAPGGRQNDTNILYFSFNRNGTIAAVTDTQ
                     PDAAPANLVEGNKLNANISFRLPDGSDNSIQLNFGTAGLVDGVTQFDQKSTTKSVWQD
                     GYAMGYMQAFNIDDSGAITGIFSNGQKRLLGQVALATFTNPGGLEKAGSNYFVASNNS
                     GNARISAALTEDKGTIRAGSLEMSNVDLSEEFTDMIVTQRGFQANSRTIQTSDQMLQE
                     VLTLKR"
BASE COUNT          470 a          551 c          574 g          390 t
ORIGIN      
        1 tattgccaga cagtttgccg cacggccagc cgtgcatccc cgtgagactg gtttgtccca
       61 cgggccggcg aagtcgcatg ttccgcatga gggtccgtcc ttcggcgaac tccttgagca
      121 ggcttcctcc acgaccggtg ccgagctcaa gttctccaaa catgcacaaa aacgtctggc
      181 ttcgcgaaac atcgatttct ccgccggaga catggaaaaa ctccagcagg caatcgatgt
      241 ggcccgaaaa aagggcgcac gggacagcct ggtcctgatg ggggatctgg ctcttgtggt
      301 cagcgtcaag aacaacacgg tgattaccgc tgtcgacgag ggatcgacaa aagaaaacgt
      361 ctttaccaat attgacagtg cggtcattgc caggtaggtg agtaccgcat ctgtggccgg
      421 tcccttcccg ggaagccgcg gatgcacgca gtatacaaaa ttttccacct cgggaaagga
      481 gcagtacacg ccatgatgcg ttcactctat gcaggtgtct ccggtctcaa gaatcaccag
      541 gtgcgcatgg acgttttggg aaacaacatt tccaacgtca acaccaacgg attcaagaag
      601 ggccgggtca cgtttcagga catgctctca cagacgctct ccggtgcggc acggccggga
      661 gacgacaagg gcggtgtcaa cccccagcag gtcggtttgg ggatgacgat cgcaagcatt
      721 gacacaatcc acaccaatgg aagcgtccag gtgacgggga agaataccga tctcgcgatt
      781 ctcggagagg gattcttcat caacaagaag gggaacgaga ccttctatac ccgtgccgga
      841 aacttcaaca tcgaccggga cgggatgctc gtcaatccgg ccaacgggta caaggtccag
      901 ggttggcagg cccagcgcgg ggcaagcggg gagttcatgg tgaacacctc gaccacgacc
      961 ggagatctgc ggataccggt cggcgaaaag gatgccgcca aagcgaccag tatggtcgat
     1021 ttcaagtgca acctcaattc cttgaccccg gttgtcaatc ccaatgccga gctttcgccg
     1081 gatttcgtgt tcaagaattc ccacagcgct gaggcactcg tctatgacaa ctatggacag
     1141 gcccacaaga tgacggtcaa cttcacgaga cagggtgtca atatctggcg cgcccagttc
     1201 acggtggctg acggaagcaa tgtggtgacg gatgcacccg ggggacggca gaacgatacc
     1261 aatatcctgt atttttcgtt caaccggaac gggacgatcg cggcggtgac ggatacccag
     1321 ccagacgctg ctcccgccaa tcttgttgaa ggcaacaagc tgaatgccaa catctccttc
     1381 cggcttccgg acggttcgga taactcgatc cagttgaatt tcggaaccgc cggtctcgtg
     1441 gatggtgtga cccagtttga ccagaagtcg acgaccaagt ctgtctggca ggacgggtac
     1501 gccatgggct atatgcaggc cttcaatatt gatgattcgg gcgccattac cgggattttc
     1561 tccaacgggc agaaacgtct tctgggacag gtcgctctgg cgacctttac caacccgggt
     1621 ggtctcgaga aggccggctc gaactatttc gttgccagca acaactcggg gaatgcccgc
     1681 atctcggcgg cactgaccga ggacaaggga acgatccggg ccgggtccct tgaaatgtcc
     1741 aacgtcgatc tttcggaaga gttcaccgac atgatcgtga cccagcgcgg gttccaggcc
     1801 aacagccgga ccatccagac cagtgaccag atgttgcagg aagtgttgac gctcaaacgc
     1861 taaggtttgt agccaactga acggggaggg gccggccagt gtgtcggtct ctccctgttt
     1921 tttgggcata ctctcatgaa attgtgatgc gactccggga aacatgtggc aaacacagga
     1981 ccgga
//
LOCUS       DAOSHE010000415         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57441_length_1982_cov_3.589517, whole genome shotgun sequence.
ACCESSION   DAOSHE010000415 DAOSHE010000000
VERSION     DAOSHE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57441_length_1982_cov_3.589517"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..937)
                     /locus_tag="PLD82_09525"
     CDS             complement(224..937)
                     /locus_tag="PLD82_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65660.1"
                     /translation="MNDIRIDGTGTIGAGEYGLVKVDGVGRCKGDLRAESVHVDGIFR
                     CRGGIHTRRLDCDGVVRMHGPLLAGWIGIDGMLRVEGSLTTEAMECDGMLTVYGDVKA
                     RELDVDGLMTVRGGTKIEATKITCEGMIRLNGEISADSIEADGFIRAKEIVGETVRIK
                     SRRFGLFAFVMRRSSRIPLIEAITIELQGVRAETVNGQDVTIGRGCRIERLDCSGNLR
                     IARGAKVKTLTGGYTLHKE"
     gene            complement(979..1584)
                     /locus_tag="PLD82_09530"
     CDS             complement(979..1584)
                     /locus_tag="PLD82_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017252427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HOG65661.1"
                     /translation="MEQETDLISKKDLLSETGISYGQLYRWKRERLIPEEWFIKQASF
                     TGQETFFPREKILKRVQEILALKERHSLDELAGMLSPDAATSMGIPASDDPLLARLPA
                     LLGRDAVSAGEAAFTWGLAQIGRLEHVPQKSLEDLVKRTIPVLVGLKLVDTVCTVYRL
                     GSTWHAVFTRGSTRPLFDSGVTVSGSFTLEEAISDMRASPC"
     gene            1706..>1982
                     /locus_tag="PLD82_09535"
     CDS             1706..>1982
                     /locus_tag="PLD82_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65662.1"
                     /translation="MPKSIRLVCLSTCLLILPGLLGAATTPRAALWIDGRTLVPRERV
                     INTGQALEFRLRHSPLTPRIPVILDADTLSQALGKARKAGCRELFVLN"
BASE COUNT          433 a          638 c          487 g          424 t
ORIGIN      
        1 tgcttcgggt caacgagtcc atctccggct ggctcgaccg gacatgcaca acctccctcg
       61 acttggcgtt ctccctgaca tggaccatcg gagcaggcag atagtttgta caaatacttt
      121 ccgatacgga catacggatt cgtacagcct gccgtaatca gagaaaacga cgcattcctg
      181 ccgtcacgga acgcgccaga agcctgcagt cggttcgtgt gactcattcc ttgtgcagag
      241 tgtacccacc cgtgagggtc ttgaccttgg ccccccgggc gatacgcaga tttcccgagc
      301 agtccagacg ctcgatccgg cagccccgtc cgatcgtgac atcctgccca ttcacggtct
      361 cggcccggac accctgcaac tcgatggtta tggcctctat caagggaatc cgggacgaac
      421 gccgcatcac aaaagcaaac aggccgaagc gtcgcgactt gatccggacg gtttccccga
      481 cgatctcttt ggcccggatg aacccgtcag cctcgatcga atccgccgag atctctccgt
      541 tcagacggat catgccctca caagtgatct tcgtagcctc gatctttgtg ccaccacgaa
      601 cggtcatcag cccgtccaca tccagctctc gggccttgac atccccgtac accgtcagca
      661 tgccgtcaca ttccatggcc tccgtggtga gggatccttc aacccggagc atgccgtcaa
      721 tcccgatcca tccagccagg agaggcccat gcatcctgac gacaccatcg caatccagcc
      781 gtcgggtatg aataccaccc cggcagcgga atatcccgtc gacatgcacc gactcggccc
      841 gtagatcccc cttgcagcgc ccaacgccgt ccaccttgac caagccatat tctcccgctc
      901 cgattgttcc tgttccgtca attctgatgt cattcattat tttgtccttt ctgttatgga
      961 atgaaataca gagtgtcatc agcatggact ggccctcatg tcactgatgg cttcctccag
     1021 ggtaaacgaa ccggagacgg tcactccgga atcaaaaaga gggcgagtgc ttccccgggt
     1081 aaacaccgcg tgccaggttg aaccaaggcg atagacagtg cagaccgtgt ccacaagttt
     1141 gagtccgacc aggaccggaa tcgtccgttt gaccaggtct tcaaggcttt tttgaggaac
     1201 atgctccaac cgtccgatct gcgcgaggcc ccaggtgaag gcggcctcac cggcggaaac
     1261 cgcatcccgt ccaaggaggg cagggagcct ggcaaggagc gggtcatccg aagcgggaat
     1321 ccccatggaa gtcgccgcat ccggagagag catgcctgcc aactcatcca atgagtgccg
     1381 ctccttgagc gccagaatct cctggacccg tttgagaatc ttttcccgag gaaaaaacgt
     1441 ctcctgcccg gtaaacgaag cctgcttgat gaaccattcc tccggaatca agcgctcccg
     1501 tttccagcga tagagctgtc cgtaggagat cccggtttcg gacaaaagat cctttttcga
     1561 tatcaagtcc gtttcctgtt ccatacgcac ctcctttgca gaacaatgtt ttgtttcagc
     1621 aacatgcaca acataacatt gtttcgacat aaaacaacat tttttctttt ttttcttcac
     1681 gagacgactc atgctagaat acggtatgcc caaatcaatc cgactcgtct gcctgagcac
     1741 atgtctcctg atcctgcccg gcctccttgg tgcggccacg accccgcggg ctgctctctg
     1801 gatcgatggc cggacactgg tcccccgcga acgtgtcatc aacaccgggc aggcgctcga
     1861 attcaggctg cgccattcac cgctcacccc gagaatcccg gtcatcctcg atgccgacac
     1921 cctgtcacag gcacttggaa aggcacggaa ggctggatgc cgggaactct ttgtcctgaa
     1981 tg
//
LOCUS       DAOSHE010000416         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_57484_length_1981_cov_4.198339, whole genome shotgun sequence.
ACCESSION   DAOSHE010000416 DAOSHE010000000
VERSION     DAOSHE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57484_length_1981_cov_4.198339"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLD82_09540"
     CDS             <1..212
                     /locus_tag="PLD82_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65663.1"
                     /translation="HELEDREGTRPVSFAGEGQTAVRESSPQLSLFGAREEAVIERIR
                     AVQPDHMTPMDALALLAELRDELGG"
     gene            complement(282..911)
                     /locus_tag="PLD82_09545"
     CDS             complement(282..911)
                     /locus_tag="PLD82_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65664.1"
                     /translation="MNINSDNIIKDAQRLRGTTDEVRSKRSSSEQGASQPAAHQIEVT
                     LDQMNINLRAHQQTVARLQLESIGLEQIQRSLEFITKNNPGDFEDIRRAIGEVQTATE
                     ATRFQQQHLIPQTLREALYSDLTASGKAQSALTLLQARRGEIETGLKDEFAQISRIQV
                     SFENIQSVNMQLDGKAQSVVEDLSRQLLAQKQLVQSPMDPATVMNLLRA"
     gene            1164..1847
                     /locus_tag="PLD82_09550"
     CDS             1164..1847
                     /locus_tag="PLD82_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOG65665.1"
                     /translation="MKPWIPGCCELCRRPIEAGAKSLCQACFSRLLDESRASPVRCPA
                     CLEYRAPLADGICPVCHEGEGPVYEVRSLARYLEPWPGLIQAVKFGQKAPLLKDLGRL
                     MGLCLGPPEKDELVTWVPLSPGRFMLRGFNQAARLARAYAESTQSRAWPLLRRIRGTK
                     AQARLGARGRRHNLDGAFALLRGGNVEGRRILLIDDVVTTGATTTACCALLTAAGAAR
                     VRIFSLCCG"
BASE COUNT          403 a          504 c          618 g          456 t
ORIGIN      
        1 tgcatgaact ggaagaccgt gaagggacgc gaccggtctc atttgccggt gaaggccaga
       61 cggctgtccg cgagtcatcc ccgcagctct ccctctttgg ggcacgggag gaggccgtca
      121 tcgagcggat ccgtgcggtg caaccggatc acatgacccc gatggatgct ttggcactcc
      181 ttgccgagtt gcgggacgaa ctcggggggt gagtgcgcga ggggggaaaa aaagacggcc
      241 tcttgcgaag ccgtcccgca ttgcctctcc cgccaggccc atcaggcccg aagcaggttc
      301 atgacagtgg ctggatccat cggtgactgg acgagttgtt tttgggcaag gagctgccgc
      361 gaaagatctt ccacaacgga ctgcgccttg ccgtcgagct gcatgttgac ggactggatg
      421 ttttcgaagg aaacctggat cctgctgatc tgggcaaatt cgtctttgag tccggtttca
      481 atttcgccgc gacgtgcttg caagagagtg agagcggact gcgccttgcc ggaggctgtc
      541 agatcggaat agagggcttc acgcagggtc tgcgggatca ggtgctgttg ctggaaccgg
      601 gtcgcttcag tggcggtctg gacttctcca atcgctcgcc ggatatcctc aaaatcaccc
      661 gggttgtttt tggtaataaa ctcgagactg cgctgaatct gttccaaacc gatgctctca
      721 agctgaagcc tggcaacagt ctgctggtga gcacgcagat tgatgttcat ctggtccagg
      781 gtgacttcga tctggtgggc agcaggctgg cttgctccct gctcgcttga cgaccgcttg
      841 ctccgcacct cgtctgttgt tccacgaagt cgttgcgcat ctttgatgat gttgtcggag
      901 ttgatgttca tggtgtatca acctcctttt tgatactacc tgcggggaaa cccccgggaa
      961 ttggtcctga cccgtcgggt cggaagcacc gcctgtgcat ccggtaaaaa tttttgaaga
     1021 ccaacctact tcattatcgg cagagaatgc ctgaaaatca agcatttttt tttgagattc
     1081 aatacaaatc ctgaaattat tttgctgaaa ctgcaaatcg gacaaggggt ggcacgttct
     1141 tgatggtatg gaggtgcaac cagatgaaac catggatccc cggatgttgt gaactttgcc
     1201 gacgcccgat cgaagcagga gcgaaaagtc tgtgccaggc atgtttttcc cgcctgctcg
     1261 acgagtcccg tgccagcccg gtgcgttgcc ctgcctgcct tgagtaccgg gcgccgttgg
     1321 cagatggaat atgcccggtt tgtcacgaag gggaagggcc ggtctatgaa gttcgcagtc
     1381 tggccaggta tctcgaaccc tggcccggct tgatccaggc ggtcaaattc ggtcaaaagg
     1441 ctcccctctt gaaggatctg ggacggctga tggggctttg tctcggaccg cccgagaagg
     1501 acgaattggt gacgtgggtc ccgctttcgc cgggacgatt catgctgcgt ggattcaatc
     1561 aggcggcccg actggcgcgg gcttatgcag aatcgacgca aagccgggcc tggccactct
     1621 tgcggaggat ccggggaacc aaggcacagg ccagattggg agcacgggga cgaaggcaca
     1681 atcttgacgg tgcgtttgct ctgctcaggg ggggcaacgt tgaaggacgg aggatcttgc
     1741 tgatcgatga tgtcgtcacg accggggcca ccacgacggc atgctgtgcc ctcttgacgg
     1801 cggccggggc tgccagagtg agaatcttct cgctgtgctg tgggtaaaaa agtatctgta
     1861 tttctacgga aagtgggtaa atattataca ccttgtactg ggatatgggg agacttgggt
     1921 cgattacgag ttgcatcgca attaatgtaa ccgaaggggg tagtccaaaa gggtcgttgc
     1981 a
//
LOCUS       DAOSHE010000417         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_58185_length_1965_cov_3.827225, whole genome shotgun sequence.
ACCESSION   DAOSHE010000417 DAOSHE010000000
VERSION     DAOSHE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58185_length_1965_cov_3.827225"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLD82_09555"
     CDS             complement(<1..268)
                     /locus_tag="PLD82_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG65666.1"
                     /translation="MSAVLHRQLAQSIVVNSIIGEGTKFRGDFELNGLLRIDGAFEGT
                     IESEGKVLVGRHGSAIAPFIKARAVVIGGKVKGNITATEMVTILS"
     gene            complement(362..1384)
                     /locus_tag="PLD82_09560"
     CDS             complement(362..1384)
                     /locus_tag="PLD82_09560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG65667.1"
                     /translation="MYTPYRKKKFKSPLMNRMHEHYMRFNKKFKAWWKKFMEKGHERL
                     TLMLIPHSEKRIFNFQISNFTISFIVVLLVVVVFFSILSLNDHETSQSQISKLSALSK
                     TREGQIDAFRTRSNYTLKNFSRFEQQIERLASSVGVENVKAVFPFYGQGGLDYTVTPE
                     MQKKYGKDFSLPDEIKELDNLNRNVARSTEQLKRVNSFIDNLKQVMNYTPSLWPVAGG
                     GFITSPFGSRPSPFTGIPMMHTGIDIAWWPGSPIKSTAAGTVTYAGFMGGYGLAVRIS
                     HKYGFSTLYAHMQNARVNVGDTVQKGQIIGSVGNTGLATGYHLHYEVILGDTQINPEP
                     YLTSKF"
     gene            1491..>1965
                     /locus_tag="PLD82_09565"
     CDS             1491..>1965
                     /locus_tag="PLD82_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG65668.1"
                     /translation="MLFDSHAHLDMLTDDPGELERILSEDCAGGLAAIVQVSTDPDIH
                     FNIYSKLHESRWNIHFACGLTHAFSGDVKKGLDKLTEIVTSRQVCALGEIGLDFSRGP
                     EPFAMEWQKNLFVEQMAIAQGAGLPVIVHTRDAAAETLAVLDRYQNVRGVAHCFTG"
BASE COUNT          429 a          490 c          580 g          466 t
ORIGIN      
        1 tcgagaggat ggtgaccatc tcggttgcgg tgatattgcc cttgaccttg ccgccaatga
       61 caacggcccg ggccttgata aagggtgcga tggctgagcc gtgtcggcca acgagaacct
      121 tgccttcgct ttcgatggtt ccctcaaagg caccatcaat gcgcaaaaga ccgttcagtt
      181 caaaatcacc gcggaatttt gtcccttcgc cgatgatact gttgacgacg atactctggg
      241 caagttgcct gtgcaggacg gcggacatgg aaaaaacctc tcttgtgcgg atacatcaaa
      301 aaccccgcag cggacccgtg caagagccgg ggccgctcgc gcggggtgat gggcaggatg
      361 atcagaattt gctggtcagg tagggctccg ggttgatctg ggtatccccg aggatgactt
      421 cataatgcag gtggtagccc gttgccagtc cggtgtttcc gaccgaaccg atgatctgac
      481 ccttctgtac agtatcgccg acgttgacgc gggcattctg catatgggcg tagagggtcg
      541 agaagccata cttgtgcgag atgcggacag ccagtccgta tccgcccata aaaccggcgt
      601 aggtgactgt tccggcagcc gttgatttga tcggactgcc aggccaccag gcgatatcga
      661 tgccggtgtg catcatggga atcccggtaa agggtgacgg gcgtgaaccg aagggcgatg
      721 tgataaagcc gccaccggcc accggccaga gggacggtgt gtagttcatg acctgtttga
      781 ggttgtcaat aaaggagttg acacgcttga gctgttctgt gctgcgagcc acattcctgt
      841 tgaggttgtc aagttccttg atctcatcgg gaagggagaa atccttgccg tactttttct
      901 gcatctcggg agtgacggtg tagtccagtc cgccctgacc gtagaagggg aagacggctt
      961 tgacattctc gacaccgacg cttgaagcca aacgttcgat ctgctgctcg aagcgggaga
     1021 aatttttcag ggtgtaattg cttctggtcc ggaaagcatc aatctggcct tcgcgggttt
     1081 ttgaaagggc ggagagtttg gagatctggg actgactggt ctcgtgatcg ttgagtgaga
     1141 gtatcgagaa gaaaacaacg acgaccagga gaacaacgat gaaggagatg gtgaagttcg
     1201 agatctggaa gttgaaaatc cgtttctcgg agtgcgggat gagcatcagg gtcaggcgct
     1261 cgtggccctt ctccatgaac ttcttccacc aggccttgaa ttttttgttg aagcgcatat
     1321 agtgctcatg catgcggttc atcagcgggc ttttgaactt ctttttgcga tagggtgtgt
     1381 acattgggac ctcgctgccg gttttgcgta ttatatcact cgcggcctgt ctgtcaatga
     1441 cagtccccgt ttttgtcctt gcactccaac accgggacgg tacacccctg atgctgtttg
     1501 actctcacgc ccacctcgat atgctcaccg acgatccggg agaactcgaa cgcattctct
     1561 cggaggattg cgccggtggc cttgcggcca tagtacaagt atcgacagat ccagacatac
     1621 acttcaatat atattccaaa ctgcacgagt caagatggaa tatccacttt gcctgcggtc
     1681 tgacgcacgc cttttccggt gatgtcaaaa aaggcctaga taaacttaca gagatagtca
     1741 cgtccaggca agtctgcgct ctgggcgaga tcgggctcga tttttcccgg gggccggagc
     1801 cttttgccat ggagtggcaa aaaaatcttt ttgttgaaca gatggccatc gcgcaggggg
     1861 cggggttgcc cgtgattgta cacaccaggg atgcggccgc tgaaacactg gccgtgctgg
     1921 acaggtacca gaatgtccgg ggggtagccc attgctttac cggag
//
LOCUS       DAOSHE010000418         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_58680_length_1954_cov_2.875724, whole genome shotgun sequence.
ACCESSION   DAOSHE010000418 DAOSHE010000000
VERSION     DAOSHE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58680_length_1954_cov_2.875724"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLD82_09570"
     CDS             <1..204
                     /locus_tag="PLD82_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG65669.1"
                     /translation="LQMQAVSISIAQQIPAGVVLVKAVSLLQLPMAMLFGRMVLKERI
                     SPRRAVGSLVMLAGAVIIVLGRS"
     gene            complement(369..1124)
                     /locus_tag="PLD82_09575"
     CDS             complement(369..1124)
                     /locus_tag="PLD82_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG65670.1"
                     /translation="MIRIVSSISIAIVLVFSLFACGKSGSSADKTIRVGAETTFPPLE
                     FKEGNEYTGFDMELIRAIGKQIGMDVTIESMGFDGLIPALQAGNIDVIISAVTITDER
                     KGKVDFSDPYYNSGLAIVVSKDNTAITSFKDLEGKKLAVQIGTTSATEAKKIKNAVVR
                     EFNSTSDAYLELKAGGADAVVNDLPVSQDFLNKGGNAYAKLVGDVLNSEQYGIAVKKG
                     NADLLKKINAALAELKKNGEYEKLYVKWFGVKP"
     gene            complement(1198..1725)
                     /locus_tag="PLD82_09580"
     CDS             complement(1198..1725)
                     /locus_tag="PLD82_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG65671.1"
                     /translation="MNLLTVFLVFFKIGLAGFGGGNAMIPLIHEQLVHQHGLLDEASF
                     ANLVALSQSTPGPFVLNIATWVGYREAGWAGALTASLAVCLPGFVLTFFGAMLLARSR
                     SNRFVQHTLVWLQPVFAGLIGATVINLLPQGIADPLQAALALVCFIAVGIGNVHPMVM
                     LGLGALAGILAAWPF"
     gene            complement(1722..>1954)
                     /locus_tag="PLD82_09585"
     CDS             complement(1722..>1954)
                     /locus_tag="PLD82_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65672.1"
                     /translation="APLIAAAMYGLRPVAIGLIASSLWRLSNTWTGGVFRPIVAGLTL
                     ILLASGLIPPMVLILIAVIAAGWHAWRTRKCA"
BASE COUNT          414 a          524 c          608 g          408 t
ORIGIN      
        1 ctccagatgc aggccgtctc catctcgatc gcccagcaga ttccggccgg tgtggtcctc
       61 gtcaaggctg tctccctgct gcaattgccc atggccatgc tcttcggacg gatggttctc
      121 aaggagcgga tcagcccgcg gcgcgcagtg gggagccttg tgatgttggc tggagcggtg
      181 atcatcgtgc ttgggcggag ttgatgctgc gcactctcct tggcagcagc cggatcactg
      241 tttggtgttt tttggtgaac accctcaggc catgaagggg ctgatgtgtt ccagtggata
      301 ttttgtacga ccaataaaaa aagcccccgg catgcgtccc gggggcttga caggcgatca
      361 aggtctgatc acggcttgac gccaaaccac ttgacgtaga gtttttcgta ctcaccgttt
      421 ttcttgagct cggccagggc cgcgttgatc tttttgagaa gatcggcatt cccttttttg
      481 accgcgatgc cgtactgttc ggaattgagg acgtcaccga ccagtttggc gtaggcattc
      541 ccgcccttgt tgaggaagtc ctggctgacg gggaggtcgt tgacgacggc atccgcgcca
      601 ccggctttga gttcaagata ggcatcggac gttgaattaa attcccgcac cacggcattc
      661 ttgatcttct tggcctcggt cgcactggtt gtcccgatct ggacggccag cttcttccct
      721 tcgagatctt tgaaggacgt gatggcggtg ttgtcttttg aaacgacgat ggccagtccg
      781 gagttgtagt agggatccga gaaatcgacc ttgcccttgc gttcgtccgt gatggtgaca
      841 gccgagatga tgacatcgat attgccggcc tggagagctg gaatcaggcc atcgaatccc
      901 atgctctcga tcgtgacgtc catcccgatc tgttttccga tggcccggat gagttccata
      961 tcaaacccgg tatactcgtt gccctctttg aactcgaggg gagggaaggt tgtctcggcc
     1021 ccgacacgga tggtcttgtc ggcactgctt ccggatttcc cgcaggcaaa caggctgaaa
     1081 acaaggacaa tggcaatgct gatagagctt actatgcgaa tcatgacgcc tcctgttgtg
     1141 atgatacagg agtctacacc ttgggagagg gcattggcaa gggaaatggc cggctgatca
     1201 gaatggccag gcggccagaa tccccgccag ggcacccaga ccgagcatga ccatcgggtg
     1261 gacattcccg atcccgaccg caataaaaca gaccagggcc agagctgcct gcaagggatc
     1321 agcgataccc tggggaagga gattgatgac cgtagcgccg atcaatccgg caaaaacagg
     1381 ctggagccag accagggtgt gttgtacaaa ccggtttgac cgggagcggg ccaggagcat
     1441 ggctccaaag aaggtcagga caaacccggg caggcagacg gccagggaag ccgtgagcgc
     1501 tccggcccag ccagcctccc gatatccgac ccaggtcgcg atattcagga caaaggggcc
     1561 cggagtggac tgggagaggg cgaccagatt ggcaaatgaa gcttcgtcca gaagtccgtg
     1621 ttgatggacc agttgctcat gaatgagcgg gatcatggca tttccgccac caaatccggc
     1681 cagtccgatt ttaaaaaaga cgaggaagac cgtcaggagg ttcatgcgca cttccttgtg
     1741 cgccaggcgt gccatcccgc ggcgatgact gcgatgagaa tcagtaccat gggcgggatc
     1801 aacccggaag cgaggaggat cagggtcaga ccggcgacga tggggcggaa gactccgcct
     1861 gtccatgtgt tgctgagccg ccagagggac gaggcaatca gtccgatggc gactgggcgc
     1921 agtccataca tggcggccgc gatcaggggg gcgg
//
LOCUS       DAOSHE010000419         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59325_length_1940_cov_4.223342, whole genome shotgun sequence.
ACCESSION   DAOSHE010000419 DAOSHE010000000
VERSION     DAOSHE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59325_length_1940_cov_4.223342"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLD82_09590"
     CDS             complement(<1..459)
                     /locus_tag="PLD82_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65673.1"
                     /translation="MSHIPRIFRIILALPPLLILCGSILAAVIDEARAALINREYPTA
                     ARMAATENAPAAILLQGNALRAMGRHTEAIQAYDRLTAQHPAAAERAKALFGKASSLM
                     ALKRFEEAGTILRSALARLSAPDRIKAQALVYVTMADRLVEPADKNVAPDH"
     gene            705..869
                     /locus_tag="PLD82_09595"
     CDS             705..869
                     /locus_tag="PLD82_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65674.1"
                     /translation="MQVQDISSRMPAQPSGAVNAQPPPAPEQAPPPPPPPEQTTPAPS
                     DNGQRVDVKA"
     gene            909..1655
                     /locus_tag="PLD82_09600"
     CDS             909..1655
                     /locus_tag="PLD82_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65675.1"
                     /translation="MRERCFRFFLTGLYALAVLLPSRLFSAVEDGLSPAVFFVAWDPW
                     RQTGCRAVGEQKEERGFRVQYDPWFRVARATRIEDGSPKDVWSYSWNEHGILQRAWRR
                     SGQILLESAEYDQDGRFVRLHRHNGMNDPQSSEEYVYGRDGRLMVIKTHKGKDLEMVT
                     EVRYHPSGAVFMHVMTNGGNAVIGRVLFDTDSEGRVLVERHFLWGWLVKTVRRAWCPH
                     GVLLSVEEQVPGWAERVRDPFFVEHKPSTP"
BASE COUNT          356 a          539 c          631 g          414 t
ORIGIN      
        1 atggtccggt gcgacattct tgtcggcagg ttcgaccagg cggtccgcca tcgtgacata
       61 gaccagggct tgggccttga tccggtcggg ggccgagagt ctggcgaggg cgctgcgcag
      121 gatggttccg gcctcttcga atcgtttgag ggccatcagg ctggaggcct tgccgaacag
      181 ggccttggcg cgttccgctg ctgcagggtg ctgcgcagtc aggcgatcgt aggcctggat
      241 ggcctcggtg tgtcgtccca tggcgcgcag ggcgtttccc tggaggagga tggcggccgg
      301 agcattctcg gttgcggcca tgcgggctgc cgtcgggtac tcacggttga tcagggctgc
      361 ccgcgcctca tcgatgacgg cagccaggat gctgccacag aggatcaaga gcggggggag
      421 ggcaagaatg atacggaaga tgcggggaat gtgcgacatg gaacgctcct tggcacaggt
      481 tcagaggact gttggccagc ccaatgacga gtgtaccaca aaccacacaa aaagttcgcc
      541 attcttccat atttacgctg gccaggtact ccgggactgc aaagactgca cagtgtgaaa
      601 aaattggtcg gttacccctg cggtctcctc ccgcggtatc gccttgacgg ggaagccggg
      661 cgggcgtacc ctgtagggag agaatcatac aaggaggtgt gtcaatgcag gttcaggata
      721 tttccagcag gatgccggct caaccttcag gtgccgtcaa tgcccagcca ccaccagctc
      781 ccgagcaggc accacccccg ccgccgcctc ccgagcagac gacaccggca ccttcggata
      841 acgggcagcg ggtcgatgtc aaggcgtaag acgggctttt tgggtagaca caacgggagt
      901 gccgcgggat gagggagcgg tgtttccggt ttttcctgac agggctctat gctctggccg
      961 tcctccttcc ttcccgactt ttttctgcgg ttgaagacgg gctctcaccg gcggttttct
     1021 ttgtcgcttg ggacccctgg cgtcagaccg ggtgccgggc tgtcggggag cagaaggagg
     1081 aacgcggctt ccgggttcag tacgacccat ggttccgcgt ggcccgggcg acccggatcg
     1141 aggacgggag cccgaaggat gtctggagct attcctggaa tgagcacggt atcctccaac
     1201 gggcttggcg ccgttccggg cagatcctgc tcgaatcggc cgagtatgac caggatgggc
     1261 gctttgtccg cctgcaccgg cataacggga tgaatgaccc ccagtccagc gaggagtatg
     1321 tctacggacg ggacggtcgg ctgatggtga tcaagacaca taagggcaag gacctcgaga
     1381 tggtgaccga ggtgcgctac catccctccg gtgccgtctt catgcatgtc atgacaaatg
     1441 gagggaatgc cgtcatcgga agggttcttt tcgatacgga cagtgaaggc cgggttctcg
     1501 tcgagcggca cttcctctgg ggctggcttg tcaaaaccgt gcgacgcgca tggtgccctc
     1561 acggcgtgct cctttcggtt gaggagcagg tacctggctg ggcagagcgg gtccgcgatc
     1621 cttttttcgt ggaacacaag ccatcgaccc catgatcctc gttttccttt ccggtttttg
     1681 tacggggaac agtctccagt cccgggacgg gtgaccggat cacgcaatgt ttcgggtttt
     1741 tgcctgtttc gtcctgcttg acaggcttcc ggttcatgcc caaattttat ccgccggcag
     1801 atgttccccc tgtccgtgac aggttgtctg acggtgcata ccagcaggac ggatggtcca
     1861 atggatatca agcattgtga ttttgaggaa ccccttgtag aaatccagca gcgtatcgac
     1921 gaacttggtt cgttttcaac
//
LOCUS       DAOSHE010000420         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59429_length_1938_cov_3.786511, whole genome shotgun sequence.
ACCESSION   DAOSHE010000420 DAOSHE010000000
VERSION     DAOSHE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59429_length_1938_cov_3.786511"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLD82_09605"
     CDS             <1..380
                     /locus_tag="PLD82_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG65676.1"
                     /translation="IACLMMFACAKVDYAYRHDVAGRVLDGQTGRAVPGAIVERIEDG
                     VPPDAVRDMYRRETDANGCFRFEYSGLGGKPEQCQLWMLQVQAVGYQSRTITNTVAWQ
                     PFRESNTASYGYILTNLRIIIKK"
     gene            460..1638
                     /locus_tag="PLD82_09610"
     CDS             460..1638
                     /locus_tag="PLD82_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOG65677.1"
                     /translation="MRISRVIMLFLAGVPVVMSTACSEDVLGLFGGTSSSALSQSASA
                     GSVSSTVTSISSSTSTGSASSQADSVVYNGETYTGMGTVLNSASPVRYTYDKLVITSP
                     AVARFEADGFFTLRGSNTDVSSNQYSVVYVTNLTTPSPRAYWFVRGNFEQRIWLPFGS
                     GRYRVTVHKTTMTSQNLDYEGAISGWSYNTSSVTNGVYTFIVENTRNEDGRFYYPSYF
                     VQSDHALIRSKATALLSGVSGVSNQIRRIHDFVVTNLEYDYSSISTNVGKRKRQDAVA
                     ALENRMGVCEGYAQLYAAILRSAGFEAKYLSGDAGGGHAWNTVFDGLVWRYIDTTWDD
                     PGPNSHLPTNLRHTYFWMTYEQMSADHSQESENPTRVVSRRSRGAGIEEIEVRVRTFD
                     "
BASE COUNT          430 a          482 c          567 g          459 t
ORIGIN      
        1 gaatagcgtg cttgatgatg tttgcctgtg ccaaagtgga ttacgcctac cggcatgatg
       61 tggccggtcg tgttcttgac ggacagaccg gtcgggctgt acccggagcg attgtcgaac
      121 ggatcgaaga cggggtgcct ccggatgcgg tccgggacat gtacaggcgg gagacggatg
      181 cgaacggctg tttccggttt gagtacagtg ggcttggcgg gaaaccggag cagtgccagc
      241 tctggatgct ccaggtccaa gctgtgggat atcagtccag gacgatcacc aatacggtcg
      301 cttggcagcc tttccgtgag agcaacactg ccagctatgg atacatcctg accaatctca
      361 ggataataat taaaaaatga caatataaaa gtattataga aaaaagacgt ttgtgtggta
      421 tgagtgtaca ctcatgagag atatgtacgg agggctctca tgagaatatc acgggtaata
      481 atgctgtttt tggccggtgt gccggtcgtt atgagcacag cgtgttctga ggatgttttg
      541 gggttgtttg gcgggacttc ctcgtcagca ctttcgcaga gtgcttcggc cggttcggtg
      601 tcctcgacgg tgacgagcat atcgagttct acttctacag ggagtgcatc atcccaggct
      661 gacagcgtag tctacaacgg agagacctat acgggaatgg gcacagtcct caattctgcc
      721 agtccggtcc gctatacgta cgacaagctc gtcatcacat cccccgctgt tgcacgcttc
      781 gaggcggatg gattttttac cctgcgtggg tcaaacacgg atgtcagttc aaaccagtac
      841 tccgtcgtat atgtcaccaa tctgacgacc ccttcacccc gggcctactg gtttgtgcgc
      901 gggaatttcg agcagcggat ctggttgccg tttggttcgg gacgctaccg cgtgaccgtg
      961 cacaaaacca ccatgacatc gcaaaatctt gactatgaag gtgccatcag cggatggtcg
     1021 tacaatacgt cgtctgtgac gaatggtgtg tacaccttca tcgtcgagaa tacacgaaac
     1081 gaggacggcc ggttctacta tccgagctat tttgtccagt ccgatcatgc cctgatcaga
     1141 tcgaaggcaa cagccctgct ttcaggcgtc agcggggtgt ccaaccagat ccgcaggatc
     1201 catgattttg tggtaaccaa tctcgagtat gattacagtt cgatatccac caatgtcggg
     1261 aagcgcaagc ggcaggatgc cgttgccgcc cttgaaaacc ggatgggtgt ctgcgagggc
     1321 tatgcccagt tgtatgcggc cattttgcgc tcggcgggtt ttgaggcgaa gtatctatcg
     1381 ggtgatgctg gtggtggcca tgcctggaat accgtctttg acgggttggt ctggcggtac
     1441 atcgatacga cctgggacga tccgggcccc aacagtcatc tccccacaaa tctcaggcac
     1501 acgtatttct ggatgaccta cgaacagatg agtgcagatc acagccagga gagcgagaat
     1561 ccgacccggg ttgtctcacg aagatcccgt ggcgccggta ttgaagaaat cgaggttcgt
     1621 gtgcgcacct ttgattgacc acaagtctgc gacaatgacc ataccggtcc tgcatcccgg
     1681 tatggttttt tttgatgaac atcctcaggc aggattcggg cgtgcggtat tctccgcgtt
     1741 gtctgcaggc atcgggagtg gttccgcgtg gcagggcgca gtatccaggt gaatgaacgg
     1801 gacagcgttg gccggccgag actctcggga caatggcatt gctcttgcta cacggattgt
     1861 tcgggcgtga gggtgttcaa aaaaaagagc tgacaatgct ccgtcagctc ccgagtacaa
     1921 gactgtgcaa ccccatta
//
LOCUS       DAOSHE010000421         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59439_length_1938_cov_2.932554, whole genome shotgun sequence.
ACCESSION   DAOSHE010000421 DAOSHE010000000
VERSION     DAOSHE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59439_length_1938_cov_2.932554"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="ychF"
                     /locus_tag="PLD82_09615"
     CDS             complement(<1..837)
                     /gene="ychF"
                     /locus_tag="PLD82_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG65678.1"
                     /translation="MGLSVGIVGLPNVGKSTTFNALTHARNAEAANYPFCTINANSAI
                     VPVPDERLAALAAIVNPKQIVPATVTFTDIAGLVKGASKGEGLGNQFLANIRETDAIL
                     HIVRCFDDENVIHVDGSVDPLRDIAVINTELILADMQSLEKKIERLEKQARQDKKIAG
                     QLETAKSLLSHLDGGNPASGFAGRESEDYSILNQELRFLSGKHVIYGANVDEAGLAAG
                     NAHVEAVRQFAASHKGEVITLCAKIEEELIDMDEAERAEYLEALGVRESGLDQIIHAG
                     YHA"
     gene            complement(1046..1900)
                     /locus_tag="PLD82_09620"
     CDS             complement(1046..1900)
                     /locus_tag="PLD82_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65679.1"
                     /translation="MILVPFEGFSLAGEMGLMIPVIERISQTWHPWDSAYFSKQEISS
                     YGAWGFLIGTTVRWEMSKETKLNLRFLYRHQDYSIKTEDRLDNNGDNEYMIDPSDRNI
                     TDWHIRRYNTWQAGFSASHTLKFGNDISLNAFVGTYLKVVYHDIRSLGTRGDNNTATT
                     GDNYVDPYQLEVTESLVPIIIGGEAKLSSWLKARFSAVRNIVDHWSEETTLRNFTAGV
                     NSVITYSEYTREFKRAPLTQLNIGATLELSGFTMDWLIKKSWFGKGGALIGEEEAIST
                     QFSITFLM"
BASE COUNT          396 a          536 c          536 g          470 t
ORIGIN      
        1 cgcgtgatac ccggcatgga tgatctggtc gagtccactc tcgcggacac ccagggcttc
       61 aaggtactcg gcccgttcag cctcatccat gtcgatcagt tcttcctcga tcttcgcgca
      121 gagggtgatg acctcacctt tgtgggatgc ggcaaactgg cgcacggctt ccacatgcgc
      181 gtttcccgct gcaagccccg cctcatccac gttggcccca tagatcacgt gctttcccga
      241 caaaaagcgc agctcctgat tgagaatgct gtagtcttca ctttcacggc ccgcaaatcc
      301 tgaagccggg ttgccgccat ccagatgtga caagagggat ttggctgttt caagctgtcc
      361 agcaattttt ttgtcctgtc tggcctgctt ttctagtcgt tcgattttct tttcgagtga
      421 ctgcatgtcg gccagaatca gctcggtatt gatgaccgcg atgtcgcgca gcggatcgac
      481 cgagccatca acatggatca cgttttcatc atcaaaacag cggacgatgt gcaaaatcgc
      541 gtccgtctcg cggatgttgg ccaaaaactg gtttcccagg ccttcaccct tggatgcgcc
      601 cttgaccagc ccggcgatat ccgtaaaggt gaccgtggcc ggcacgatct gtttcgggtt
      661 gacgatcgcc gccagcgcgg ccaagcgctc gtccggtacg gggacgatgg cggagttggc
      721 attgatggtg cagaacggat aattggcagc ctcggcattc cgggcatgcg tcagtgcatt
      781 gaatgtggtc gatttgccga cgttgggcag gccgacaatt ccaacggaga gtcccatgat
      841 tccatgatcc ttgagtggta tgggttcaaa aatacccaaa atcccgtact ccgtcccgca
      901 ccccgatcat gtacgagaat gtttatagaa gaaaagacac ccgctcgagg tgtctttctt
      961 ttttgtgaac accctcacgc cccgaagaat cgcatccaac aggactgagg atgttcagca
     1021 aactcgtctg tctgcaaacg gacgcctaca tcaagaaggt gatggagaat tgtgtcgaga
     1081 tggcttcctc ttctccgatc agcgcgccgc cctttccgaa ccagctcttt ttgatcagcc
     1141 agtccatggt gaatcccgaa agttcgagtg tcgcaccgat attcaattgt gtgagggggg
     1201 cccgcttgaa ctcgcgggtg tattcgctgt acgtgatgac gctgttgacg cccgcagtaa
     1261 aattccgtaa ggttgtttcc tcgctccagt ggtcgacgat gttacggacc gccgagaagc
     1321 gggccttgag ccaggagctg agcttggctt cgccgccgat gatgatcggg acaagggatt
     1381 cggtcacttc caactggtac ggatcgacgt agttgtcgcc ggttgtggcc gtattgttgt
     1441 ccccgcgggt tccgagactg cggatgtcgt gatagacaac cttgagatac gttccgacaa
     1501 aggcgttgag ggagatgtcg ttgccgaact tgagagtgtg actggccgag aagccagcct
     1561 gccaggtgtt gtagcgccgg atgtgccaat ccgtaatatt acggtcactc ggatcgatca
     1621 tgtattcatt gtcaccgttg ttgtcgaggc ggtcctcggt cttgatggag tagtcctggt
     1681 ggcgatagag aaaccgcaga ttgagtttgg tttccttgct catttcccag cggaccgttg
     1741 tcccgatcaa aaatccccaa gcgccgtacg aggagatctc ctgcttgctg aagtaggcac
     1801 tgtcccaggg atgccaggtc tggctgatcc gctcgatcac gggaatcatc agtcccatct
     1861 ctccggccag gctgaaccct tcgaatggaa ccaatatcat ccgtgttgtt gcccgctcca
     1921 aggatacgac ctacagcc
//
LOCUS       DAOSHE010000422         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59814_length_1930_cov_3.849600, whole genome shotgun sequence.
ACCESSION   DAOSHE010000422 DAOSHE010000000
VERSION     DAOSHE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59814_length_1930_cov_3.849600"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1579
                     /locus_tag="PLD82_09625"
     CDS             269..1579
                     /locus_tag="PLD82_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG65680.1"
                     /translation="MKRPYISLLFVLFLLLSATSLEAVVFRWAVEKDAAYRVLSYVNE
                     QRFLNGRYHSTIEIRNKAVLKTIAVSSNGQSGLYKGEFQYFEREVGHTNEPFALKEVY
                     PSEFWRDRLGKYTIDRDYFMPVVRDVPIFPEKDIAPGEMWQADAHEVHDLKEYGLTSA
                     YRIPIKARYIYVGDRVIEGRRLALFSITYVFSHYGDIDLDQYVTRANQDLAKYRNDPA
                     RHAALRQRYEYQLERMRRAPQRINGSCIQLYYWDIEKGLPVSMNEDFHFIFHLLNGEV
                     HEFKGISSAAFERIKPVGEKEADRITDTLKGREGDGITVKRDKRGIVVELGDILFDTD
                     KFTLKPDARATLMGLAERIKQSGKFEIRVEGHTDNTGDPGYNLDLSRKRANVVANFLT
                     KAMGTDPRSISWIGYGMSRPAADNRTISGRAKNRRVEIILLTQE"
     gene            complement(1708..>1930)
                     /locus_tag="PLD82_09630"
     CDS             complement(1708..>1930)
                     /locus_tag="PLD82_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65681.1"
                     /translation="EPAVLPATVVRATPVDHGFEIAVSFNNEETALRARLVEQIVRID
                     DYRRSRGIEDFDAAADEWIRKYSENFPYD"
BASE COUNT          418 a          528 c          571 g          413 t
ORIGIN      
        1 tgcacccaac cgtttttgaa cggacgcagg ccttcggatt catcatggtg aataccctct
       61 atgccctcgt cgacgggttc ctcctcggat tggctctgtc ctttctgtac aacctcttca
      121 tcaggaagga acgggcagcg ggggagtagt ttcttcaggg ccgggccggc ttgttttgcc
      181 gatacttgaa gagaggcttc gcccgcccgc tgcgttcagg tgattccctg cctgaggggg
      241 tgtctttcct gaaagcaagg agaaccagat gaagcgtcca tatatcagcc tgctgtttgt
      301 gctgttcctg ctcttgtccg caacgagtct tgaggccgtc gtcttccgct gggccgtcga
      361 gaaggatgca gcctatcgcg ttttgtccta tgtcaatgag caacgatttc tcaacggtcg
      421 ataccacagc acgatcgaga tccggaacaa ggcggttctg aaaacgatcg ccgtcagcag
      481 caacggccag tccgggctct acaagggcga gttccagtat ttcgagcggg aggtcgggca
      541 caccaacgag ccgttcgccc tcaaggaagt ctatcccagc gagttctggc gggacaggtt
      601 ggggaaatac accatcgacc gggattactt catgccggtg gtacgcgatg tcccgatttt
      661 ccccgaaaag gatatcgccc ccggcgagat gtggcaggcc gatgcccacg aggtgcacga
      721 tctcaaggaa tacggcttga ccagtgcgta tcggattccc atcaaggccc gctatatcta
      781 cgtcggggac cgtgtcatcg agggtcggcg tctggcactc ttttccataa cgtacgtctt
      841 cagccattac ggggatatcg atctggacca gtatgtgact cgtgccaacc aggatcttgc
      901 caagtacagg aatgatccag cccggcatgc ggctctccgt caacggtatg agtaccagct
      961 cgagaggatg cggcgggccc cccagcgcat caatggatcc tgcatccagc tctattattg
     1021 ggatatcgaa aagggtcttc ccgtcagcat gaacgaggat ttccatttta tcttccattt
     1081 gctgaacggc gaagtgcacg agttcaaggg catctccagc gccgcatttg aacggatcaa
     1141 gcccgtcggt gaaaaggaag cagaccggat caccgatacc ctcaagggcc gcgagggcga
     1201 cgggatcacc gtcaagcggg acaagagggg gatcgttgtc gaattgggtg atatcctctt
     1261 tgataccgac aaattcaccc tcaagccgga tgccagagcg acgctcatgg gccttgcaga
     1321 gagaatcaaa cagtccggga aattcgagat ccgcgtcgag ggacataccg acaatacagg
     1381 agatccgggc tacaacctgg atctgtcacg gaagcgggcc aatgtggtgg cgaatttctt
     1441 gacaaaggcc atgggaaccg atccgcgttc catatcctgg atcgggtatg gaatgagccg
     1501 gcccgccgcc gacaacagga cgatcagtgg gcgggccaag aatcgccggg tcgagattat
     1561 ccttctgacc caggagtgag cagcgtctga agagtcgctc gcggtatgca aagaacccgg
     1621 cattttcctg ccgggttctt tgcattgtgt ctgtttcagg ggtctgaacc gggagagtgc
     1681 cgtgccctgt gttgagccaa agatgagtca gtcgtacggg aaattctcgg agtacttgcg
     1741 gatccattca tctgcggccg catcgaaatc ctcgatcccg cgggaacggc ggtagtcgtc
     1801 aatccgtacg atctgctcca caagcctggc cctgagagcg gtttcctcgt tgttgaagga
     1861 gacggcgatt tcaaagccgt gatcgacggg ggtcgcgcgg acaacggtgg cagggagaac
     1921 cgccggttcc
//
LOCUS       DAOSHE010000423         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59818_length_1930_cov_3.371733, whole genome shotgun sequence.
ACCESSION   DAOSHE010000423 DAOSHE010000000
VERSION     DAOSHE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59818_length_1930_cov_3.371733"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..1347
                     /gene="prmC"
                     /locus_tag="PLD82_09635"
     CDS             436..1347
                     /gene="prmC"
                     /locus_tag="PLD82_09635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG65682.1"
                     /translation="MMTIGGALQEARDRLQAREGWRLDAEVLLCHVLGCRRIDLVTRS
                     EEELDGEPSMRFAAALARRAAHEPVAYITGEREFYGRPFLVGPGVLIPRPETEALVDA
                     VLSWAAGRAEGLRVLDIGTGSGAIALTLALEMPTADIWASDCSPNALDWAKRNACRLL
                     AETFSSAHKIDSEGQTFFPRTGRSAQVHLVSGSCYAGIEGRFDLVVSNPPYLTEDEVR
                     EAMPDVREWEPELALVARDGGLAVYRAIIKELPHRLTPGGLAAFEISDTVAGGVQDEA
                     RRAGFVCEMEDDLAGHKRVAVLRTPLE"
     gene            complement(1328..>1930)
                     /locus_tag="PLD82_09640"
     CDS             complement(1328..>1930)
                     /locus_tag="PLD82_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG65683.1"
                     /translation="PQIDFMTQLKNLCKKYDILLIADEIQTGLGRTGRFLACEHSLIT
                     PDIALLGKSLGGGLPLSAVLTTDAVAGAFGYGDHGTTMGGNPVSCAAGLACLTHILKK
                     KLIRDVASLGDDFKEALFTLKKKHPAISGIRNLGLIIAFDFEHAEALVEECLANGLII
                     NKVQQGTIRLLPPFNLGAGELKLAMGILDKALKKVTPKEF"
BASE COUNT          365 a          518 c          675 g          372 t
ORIGIN      
        1 ggccgcactc gaaaaagaca ggcgggagac ccttttagcc gagattgccc gatcggggga
       61 gcaggtctga aggagacggg attgcccgtg caccaacgga accggtctcg aggttttttg
      121 atgaacagcc tcatgccggc ggatgcgcgg cctttctcgc gggggggggc gggcaggtgc
      181 agtacaggcc gtagggcgca ggatggcatg tgaggcacga gggactcgtg gaaggggccg
      241 ccgagagtcc cttggtcccc cgggctgcgt gagaactgtg ggtacacgga agagaaacgc
      301 ttcacctgcg gatggatccg gtgtttctgg cggtgagcac caggtgggaa gcacgatacg
      361 tgacaggaat ggctccgact ggttggcaac atggtacgga agtccattga actgatgaag
      421 ccggatggcg gaggaatgat gacaattgga ggggcactcc aggaagcgcg tgatcgtttg
      481 caggcgcgcg agggctggcg gctggatgcc gaggtccttc tttgtcatgt tcttggctgc
      541 cgacggatcg atctcgtgac ccgttccgaa gaagaactgg acggggagcc gtctatgcgg
      601 tttgccgccg ccctcgcgcg ccgcgccgcc catgaaccgg tggcctatat taccggagag
      661 cgcgagttct acggccggcc atttttggtc ggtcccggcg tgctcatccc ccgtcccgag
      721 accgaggcct tggtcgacgc ggtcctgtct tgggcggccg ggcgggcgga aggactccgg
      781 gtgctggata tcggaaccgg gagcggggcg attgccctga ccctcgccct cgagatgccg
      841 acggccgata tctgggccag cgattgttca ccaaacgccc tcgactgggc caaacggaac
      901 gcctgcaggc tccttgcgga gactttctct tctgcacaca agatcgacag cgaaggacaa
      961 actttttttc cccgcaccgg ccggagtgcc caagtccatc tggtcagtgg atcgtgttac
     1021 gccgggatcg aggggcggtt tgatctggtc gtatccaatc caccctacct gacggaagat
     1081 gaagtgcggg aagccatgcc ggatgtccgg gagtgggagc cggagttggc ccttgtggcc
     1141 cgggatggtg ggctggcggt ctatcgggcg attatcaagg aactaccgca caggctcaca
     1201 ccgggcgggc tggccgcatt cgagatcagc gataccgtgg ccgggggcgt acaggacgaa
     1261 gcccgcaggg cgggcttcgt ctgtgagatg gaagatgatc tggccgggca caagcgggtg
     1321 gccgtactca gaactccttt ggagtgacct tcttgagagc tttgtccagg atccccatgg
     1381 ccagcttcaa ttcgccggca cccagattga agggtggaag gaggcggatt gtcccctgct
     1441 ggaccttgtt gatgatcagg ccattggcga gacattcttc gaccagcgcc tcggcgtgtt
     1501 caaaatcgaa cgcgatgatc aggccgagat tgcggatccc cgagattgcg ggatgcttct
     1561 ttttgagggt gaagagggct tctttgaagt cgtccccgag actggcgaca tcgcggatga
     1621 gcttcttctt gagaatatgc gtcagacagg ccaggccggc cgcgcaggaa accggattgc
     1681 cgcccatcgt ggttccatga tcgccgtatc cgaaggcgcc ggcgacggca tccgtggtca
     1741 gaacggctga cagggggagc ccgccaccca gggatttgcc gaggagggcg atgtcagggg
     1801 tgatcaagga atgttcgcag gccaaaaagc ggccggtgcg gccgaggccg gtctggatct
     1861 cgtcagcgat caggaggatg tcatattttt tgcagagatt cttgagctgg gtcatgaaat
     1921 cgatctgcgg
//
LOCUS       DAOSHE010000424         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59975_length_1927_cov_2.342949, whole genome shotgun sequence.
ACCESSION   DAOSHE010000424 DAOSHE010000000
VERSION     DAOSHE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59975_length_1927_cov_2.342949"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..831
                     /locus_tag="PLD82_09645"
     CDS             70..831
                     /locus_tag="PLD82_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65684.1"
                     /translation="MPQTEAGIRQDVERSLDKNDIITFRTYYLSDYGEMVLRVIASSI
                     LERFGRSDLMDIVYSAAKELIINATKANLKRVVFTELGLDLLSPSEYEKGMHIFKERL
                     VEDKIKEYEKVFKEMDYPVVTTFYYWPNVLNIKVKNSFMLLPEEEKKIRSKFERAHSF
                     ANLLDFYMEYGDDTEGAGLGLAMVGILLDESGIDKHAFTLYSNQYNETAARLEIPLTK
                     DYVPRRVLFERERAKSGLSADEFRKIFQPHNLVQS"
     gene            855..>1927
                     /locus_tag="PLD82_09650"
     CDS             855..>1927
                     /locus_tag="PLD82_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG65685.1"
                     /translation="MAAFFWTQFAGAFIDNFYKNALILWISFGAPGLEPGLRNTLIIL
                     CSGIFILPFFLFSATAGWLADSQSKDRVIRWTKLAEILIMGAGAMAFLAGSIPALIIV
                     LFFMGAQSAWFGPAKYGVIPELVPEENLLRANGLVAMGTFVAILTGTLFGGLAGSLND
                     GRILAACSVVLIAVFGWLASLKIPRLGAKAPGTPRAGGWIRETGIRLGEIRRETRIFP
                     LLVAISWFWFHGAFILQLLPIHIMHAGENTILPAALDWMHGSAGMAGSQVTLSLLLFS
                     GGIGIGSMIPGRRKTRHIETASSVWALGLLSLTAMLVPLVEDIFPVVFCILALSGIAA
                     GLYSVPLYAAIQATARPAIRARI"
BASE COUNT          424 a          549 c          499 g          455 t
ORIGIN      
        1 cacatattct gtttgtgggc ctgcgggata ctgttcgcgg gcaccaagac ccgcagaaat
       61 ggatgttgta tgccacaaac cgaagcaggg atacgccagg acgtcgagcg ttccctcgat
      121 aaaaacgata tcatcacttt tcggacgtac tacctcagtg actacggcga gatggtttta
      181 cgtgtcatcg cgtcatccat cctcgaaagg ttcggacgat ccgatctgat ggatattgtc
      241 tacagtgccg ccaaagagct gatcatcaac gcgacaaagg ccaatctcaa acgcgttgtt
      301 tttacggaac tcggcctgga tctcctcagc ccctcggagt acgaaaaggg aatgcatatc
      361 ttcaaagaac ggctcgtcga agacaaaatc aaagaatacg aaaaagtctt caaggagatg
      421 gactatcccg tcgttacaac attctactat tggcccaatg tcctcaatat caaggtcaaa
      481 aacagtttta tgctcctgcc tgaggaagaa aaaaagatcc gctccaaatt cgaacgggca
      541 cactcattcg caaatctcct ggatttttat atggagtacg gagatgacac cgagggtgcc
      601 gggcttggac tggccatggt cggaatcctg cttgacgaga gcggtattga caagcatgcc
      661 ttcacactgt actcaaacca gtacaacgaa acagccgccc ggctcgagat tcccctgacc
      721 aaggactatg tcccgagacg tgttctcttt gagcgtgaac gggccaagtc cggactctct
      781 gcggacgaat ttcgcaaaat ctttcagcca cacaaccttg ttcaaagctg acatccggag
      841 ggaactgcgt gaagatggcc gcttttttct ggacgcagtt tgccggtgcg tttatcgaca
      901 atttctacaa aaacgccctg atcctctgga tcagttttgg cgcacccggg cttgaacccg
      961 gtctacggaa taccctcata attctctgtt cgggaatttt catcctccct ttttttcttt
     1021 tttcagccac agcgggctgg ctggctgact cccaatccaa agatcgtgtc atccgctgga
     1081 caaaactggc agagatcctg atcatgggtg cgggagccat ggcgtttttg gccggaagca
     1141 tcccggccct gatcatcgtc ctctttttca tgggtgccca gtctgcatgg ttcggaccgg
     1201 caaaatacgg ggtgattccc gagctcgtcc ccgaggaaaa cctgctgcgg gcaaacggtc
     1261 tggtcgcaat gggcactttt gtcgcgattt tgacggggac cctgtttggc ggcttggccg
     1321 gaagcctgaa tgatggccgc atcctggccg cctgctccgt tgtattgatc gcagtttttg
     1381 gctggctcgc cagcctgaag atcccccggc taggcgcgaa ggcccccggg acaccccggg
     1441 caggaggatg gatccgcgag accggaatcc ggcttggtga aatcagacga gaaacacgga
     1501 tctttcccct cctcgtagcc atctcctggt tctggttcca tggtgccttc atcctccagc
     1561 ttctcccgat ccatatcatg catgcggggg aaaacaccat ccttcctgcc gctctggact
     1621 ggatgcacgg aagtgcggga atggccggct cacaggttac cctttccctg ctgctctttt
     1681 ccggcggaat cgggatcggg tcgatgattc ccggcaggcg gaagacacga catatcgaaa
     1741 cagcgtcatc ggtctgggcc ctgggtctgc tgtcactgac agccatgctc gtgcccctgg
     1801 ttgaagacat ctttcccgtc gtcttctgca tcctggccct ctccggcatc gcagcaggac
     1861 tctactcggt tcctttgtat gccgcgatcc aggccacagc acgcccggca atccgggcac
     1921 gaatttt
//
LOCUS       DAOSHE010000425         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_59998_length_1926_cov_4.339925, whole genome shotgun sequence.
ACCESSION   DAOSHE010000425 DAOSHE010000000
VERSION     DAOSHE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59998_length_1926_cov_4.339925"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="murD"
                     /locus_tag="PLD82_09655"
     CDS             complement(<1..1309)
                     /gene="murD"
                     /locus_tag="PLD82_09655"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG65686.1"
                     /translation="MPLKNGSLILVVGFGLKTGVSIARFALARGVRVRAVDSKPAEKL
                     ADSMRATGNAVELVSGGMPLSALDGVSLVVLSPGVPRNGELLTEADRRGIPVIGDIEL
                     AFRYARCRMAGITGTDGKSTTTAMVGSILETASLGRVAGNIGIPVLDVVDGMAPGSIL
                     ALELSSFMLESIERFKPEVAAILNLAEDHLDRYPDMESYLAAKLRIFSNLDAGCTAVW
                     PTDNPWSGRFAGAIPAGVRRVSFALDDAAADVHLGTTGEGVEILMIAGEPCLPAGDLP
                     VMGRHNLRNAVSAAAIAFALGISRQAIADGLRAFRGLPHRFENAGTAAGVRFINDSKA
                     TTISAVATALDSAGAGSCILIGGRDKGLEFTPLRDAILRKKLEVFPFGEARDKVREQL
                     GCERTGDASLETVVRQAWAYARRNRAAEIILAPGCTSYDEFPNF"
     gene            complement(1315..>1926)
                     /gene="mraY"
                     /locus_tag="PLD82_09660"
     CDS             complement(1315..>1926)
                     /gene="mraY"
                     /locus_tag="PLD82_09660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG65687.1"
                     /translation="TKIFIPFFADLSIDLEWLYIPFAIILLVGSSNAVNLTDGLDGLA
                     IGLTLFVATAFAAIAYLTGHFNIANYLKIPFISGAAEITVFCGALIGAGLGFLWFNAH
                     PAEVFMGDTGSLALGGTLGLMALMLKKELLLILVGGVFVVEALSVIIQVTVFKLRGKR
                     VFKMAPIHHHYELKGWHENKVITRFWIIGLLLAILALATLKVR"
BASE COUNT          435 a          585 c          565 g          341 t
ORIGIN      
        1 caaaattcgg aaattcgtca tacgaagtac agcctggtgc gagaatgatc tcggccgccc
       61 tgtttctgcg ggcatacgcc caggcttgac gcacgaccgt ctcaagggaa gcatcaccgg
      121 tacgctcgca cccgagttgt tcccggactt tgtcccgggc ctcgccaaag ggaaaaacct
      181 ccagcttctt gcgcaggatg gcatcccgga gtggtgtaaa ctccagtccc ttgtcccgtc
      241 ccccgatcag gatacaggaa cccgcaccgg ctgaatcgag ggccgttgcc accgcggaga
      301 tggtcgtcgc cttggagtca ttgatgaagc gcaccccggc ggccgttccg gcattctcga
      361 aacgatgggg cagtccgcgg aaggcacgca gcccgtctgc aatcgcttga cgtgaaatcc
      421 cgagtgcaaa cgcgatggcc gcggccgaga cggcattccg gaggttgtgt cggcccatca
      481 cgggcagatc cccggccgga agacagggct cccctgcaat catgaggatc tcgacacctt
      541 cccctgtcgt cccgagatgg acatccgcag cggcatcatc cagggcaaaa ctcactctgc
      601 ggacaccggc cgggatggcc ccggcaaacc gcccgctcca gggattgtcc gttggccaga
      661 cagcagtgca gccggcatca agattggaaa aaatccggag tttggcggcg aggtacgact
      721 ccatatccgg gtagcggtca agatggtcct cggccagatt gagaatcgcc gccacctcgg
      781 gtttgaacct ctcgatcgat tcgagcatga agctcgagag ttcgagcgcc aagatggagc
      841 cgggagccat tccgtcgaca acatcaagca ctgggatgcc gatatttccg gcaacccgtc
      901 cgagggatgc cgtttcaaga atgctcccca ccatggctgt cgttgtcgat tttccatcgg
      961 tccccgtgat gccggccatc cggcaacggg cgtagcggaa cgccaattcg atatcaccaa
     1021 tcacgggaat cccgcgccgg tccgcctcgg tcaagagttc gccgtttctg gggactccgg
     1081 gcgagaggac gacgagcgag acgccatcca gggccgagag aggcatcccg ccggaaacaa
     1141 gctcgaccgc gtttccggtg gcgcgcatcg agtccgccaa tttctccgcc ggcttgctgt
     1201 ccaccgcccg caccctgacc ccgcgggcaa gagcaaagcg cgcgatggaa acgccggtct
     1261 tgagaccgaa tccgacgacg agaatcaggc ttccgttctt gagaggcatc cggactacct
     1321 caccttgagt gtggccagtg ccaggatcgc caagagcaag ccgatgatcc agaaacgggt
     1381 aatgaccttg ttctcgtgcc atcctttgag ttcgtagtga tgatggatcg gagccatctt
     1441 gaagacccgt tttcccctga gtttgaaaac ggtcacctga atgatgaccg agagggcctc
     1501 gacgacaaag acgccaccga ccaggatcag aagcagttct tttttgagca tgagcgccat
     1561 caggccaagc gtcccgccga gggccagact tccggtatca cccatgaaga cctcagccgg
     1621 atgagcattg aaccagagga acccgagacc ggccccgatc agcgccccgc agaagaccgt
     1681 gatttcagcc gccccggaaa tgaacgggat cttgagataa tttgcaatat tgaagtggcc
     1741 agtcagatag gcgattgcgg caaaggccgt cgccacaaag agggtcaggc cgatggccag
     1801 accgtccaga ccgtccgtca gattgaccgc attggatgaa ccgacgagca ggatgatcgc
     1861 aaatgggata tagagccact cgagatcgat cgagagatcg gcaaaaaacg ggatgaagat
     1921 ctttgt
//
LOCUS       DAOSHE010000426         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_60344_length_1919_cov_3.433476, whole genome shotgun sequence.
ACCESSION   DAOSHE010000426 DAOSHE010000000
VERSION     DAOSHE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60344_length_1919_cov_3.433476"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1793
                     /gene="murJ"
                     /locus_tag="PLD82_09665"
     CDS             336..1793
                     /gene="murJ"
                     /locus_tag="PLD82_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOG65688.1"
                     /translation="MTQSDKRILIKASIVIMLATLLSRVFGLVRMQVINYHFPQELLD
                     PFWAAYKIPNTLRELLAEGALSVAFIPVFSACLAKRGVEDASLLASRVLNALVLVVLA
                     VVGLGILTAPLYMPVYVSGFSTDAVRVALAVELGQLMMPFLLLASAGAVVMGVLNGRQ
                     HFAAPAFAPVMFSIGMILTVWLFGDRGVYVLGWGVLAGGVLQLLFQLPFLRGHGIRYI
                     PSLSLRDPGVREVLGLMLPAALALGVVQLNQLFVPFFASWNEGGMSALQNAILLVQMP
                     QGVFAIAISTALLPGLSRQASLGDMEGFRRGMANGLALVIFFMVPAALFFVFFRVEIV
                     DAIFNLGGKFDAKAVQATAESLYWYSLGLIGMGGTVLANRFFYGMRDTRTPLLVALAS
                     VLVNILCNVLFHLTIRDTAMIALANSIAVTVNFILLMAIISRRTGGLDWSLIGRESAK
                     ILIAASVMSVAVAAVSALFGIPETRTVSLPSCVSA"
     gene            1790..1915
                     /locus_tag="PLD82_09670"
     CDS             1790..1915
                     /locus_tag="PLD82_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65689.1"
                     /translation="MMLVVAVVSFVAVAALLKVGELGEILSMIRARLGRRGRANP"
BASE COUNT          302 a          545 c          593 g          479 t
ORIGIN      
        1 ccggggggcg aagggtgacc gcagggcgag gacgatggac ccccggggaa ggccgggcag
       61 catggagttt ccgctgacga cggaaatgtc gaaaaccaga aaccgcagga acaggaagac
      121 cgcaaaggcc gtcccgatcc agatccatgg ctgtctcatg agcttcatgg acaaaaccta
      181 tcgaaagagg gactgtcgaa caagaggctt cccgttgaat ggtggcggca cactcccgga
      241 gtgcgccaga tcccgtaatg ctgttcgggg ggcttgtccg gtttacgagg gcggaagtat
      301 gttttgaatg gtttgaaaca gtgtgggagc aggcagtgac acagagcgac aaacggatcc
      361 tcatcaaggc gagcatcgtg atcatgctgg cgaccctcct ctcgcgcgtt ttcggtctgg
      421 tccggatgca ggtcatcaat taccattttc cacaggaact cttggatccc ttttgggccg
      481 cgtacaagat tcccaatacc ctcagggaac tcctcgccga gggtgctctc tccgtggcct
      541 tcatcccggt tttttcggcc tgtctcgcca agcgaggagt tgaggatgcc tctctcttgg
      601 ccagccgggt cctgaatgcc ctggtcttgg ttgtcctggc cgtggtcggg ctggggattc
      661 tgacagcgcc gctctatatg ccggtctacg tttccgggtt ttcaacggat gccgtccgtg
      721 ttgccctggc ggtcgagctc ggccagttga tgatgccgtt tttactgctg gcgagcgccg
      781 gggctgttgt catgggagtt ctgaacggac gtcagcactt tgctgccccg gccttcgctc
      841 cggtcatgtt ctcgatcggg atgatcctca ctgtctggct ctttggtgat cgcggagtgt
      901 atgtgcttgg ttggggtgtg ttggccggtg gtgtcctcca gcttctgttc cagcttccct
      961 tcctgcgggg gcatggcatc cgctatatac cctcgctctc cctgcgtgat ccgggagtcc
     1021 gggaggtgct cggcctgatg ctgccggcgg ccctggctct gggagttgtc cagctcaacc
     1081 agctcttcgt tccttttttc gcctcatgga atgaaggggg gatgtccgcc ctgcaaaacg
     1141 cgatcctcct ggtgcagatg ccgcaggggg tgtttgccat cgccatctcg acagccctgt
     1201 tgccgggact ctcgcgacag gccagcctcg gtgatatgga aggctttcgt cgtggcatgg
     1261 ccaacggcct ggccctggtg atttttttca tggttccggc cgccctcttt ttcgtgtttt
     1321 tccgtgttga aattgtcgat gcgattttca atcttggcgg gaaattcgat gccaaggcag
     1381 tccaggcgac cgctgaaagc ctctactggt attcgctcgg gctgatcgga atggggggca
     1441 cggttctggc caaccgcttc ttctatggaa tgcgcgatac aaggacacct cttttggttg
     1501 cgctggccag cgttctggtc aacatcctct gcaacgtgct ctttcacctg accatcaggg
     1561 ataccgccat gattgcgctt gccaattcca ttgccgtgac ggtcaatttc atcctgctga
     1621 tggccatcat cagccgccgg acgggcgggc tggactggtc cctcattggg cgcgagtccg
     1681 caaagatcct gatcgcggcc tccgtcatgt cggttgctgt cgcggctgtg agcgccctct
     1741 tcgggattcc tgagacacgg acggtttcct tgccctcctg cgtttcggcg tgatgctggt
     1801 tgttgccgtt gtcagctttg tcgctgttgc ggctctcctc aaagtcggtg aactgggcga
     1861 gatcctctcc atgatccgcg cacgtttggg ccggcggggg cgggcgaacc catgatctg
//
LOCUS       DAOSHE010000427         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_60664_length_1912_cov_3.415186, whole genome shotgun sequence.
ACCESSION   DAOSHE010000427 DAOSHE010000000
VERSION     DAOSHE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60664_length_1912_cov_3.415186"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="amrS"
                     /locus_tag="PLD82_09675"
     CDS             complement(<1..964)
                     /gene="amrS"
                     /locus_tag="PLD82_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG65690.1"
                     /translation="MPAHRARWFHPETDNRVRCTLCPRSCLIPDGKTGACRVRMNMGG
                     ELVALTWGRFPALAIDPIEKKPLYHFFPGSSTLSLGSLGCNMHCLHCQNPSLSDPDVF
                     AIAHLEQAKPMPPEFIAPFAREHTVPIVAWTYNEPLVNAEYLVSVLPLVQRAGLKSVL
                     VTAGLISPEPLTEILPHLDAWRIDIKGWGDEFYQRLTGFPGWKTVLVNSLMAKEAGCH
                     IEVVTNIIDGWNDSPEMLASIAGWIAAELGAETPWHVTASHPAGRMLDLRPTPSATIV
                     QARAIGLESGLKHVYCGNIDTPDGSDTTCPDCGAILIRRRGFAVL"
     gene            complement(951..>1912)
                     /locus_tag="PLD82_09680"
     CDS             complement(951..>1912)
                     /locus_tag="PLD82_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG65691.1"
                     /translation="LKEYFTSAPGASQEKTEAIQDSALAQVCAFLSRRKKLQARPESR
                     LDLDLGLDSLDRLEMAAYLQSELGLTIAEADLARAATVAEAVSLARAATDDEVLQSGD
                     PFDGPVEMPKLRKGTRSSARALLSFFMRHLFHLRIEGLENLPGEPCIIVANHESYLDA
                     PTLIAKLPKRFLARSVTWIKASNVMERVVKFFSRGRNLIVVHAKSDLAATLRSSAAVI
                     EAGYNLMVFPEGLRSRDGGLASFRPGFAMIACRTRVPVVPVAIDGALEAMPYGKTFPR
                     FGKKICLKILSPIIPGSDETPEALADRARNSIASIVEKAHARP"
BASE COUNT          379 a          529 c          646 g          358 t
ORIGIN      
        1 cgaggacggc aaaaccccgc cggcggatca ggatggcccc gcagtccggg caggtcgtgt
       61 cactgccatc aggggtatca atatttccgc aatagacgtg tttcagcccg gactcgagtc
      121 cgatcgccct ggcttgtacg atggtggccg acggagtcgg ccggagatcc agcatgcggc
      181 cggccgggtg gctggctgtc acgtgccagg gtgtctctgc gcccagttcg gcggcgatcc
      241 agcctgcgat tgatgcgagc atctcgggcg aatcattcca gccatcgatg atgttcgtca
      301 ccacctcgat atgacaaccc gcctccttgg ccatgagact gttcaccaaa actgttttcc
      361 agcccggaaa gccggtcaag cgttgataga attcgtcgcc ccatcccttg atatcaatcc
      421 gccaggcatc cagatgaggg agaatctccg tcagtggttc aggggagatc aatccggcgg
      481 tcacgagaac gctcttcagg ccggcccgtt ggacgagggg gaggacgctg acaaggtact
      541 ccgcattgac gaggggttcg ttataggtcc aggcgacgat cgggacggtg tgctcccgcg
      601 cgaagggggc gatgaactcg gggggcatgg gcttggcctg ctcgagatgg gcgatggcaa
      661 agacgtcggg atcggagagg gaggggtttt ggcagtggag acaatgcatg ttgcacccga
      721 ggctccccag agaaagcgtg gaactcccgg gaaaaaaatg gtagagcggc tttttttcga
      781 tcggatcgat cgccagggcc ggaaatcggc cccaggtcag ggcgacaagt tccccgccca
      841 tattcatgcg cacgcggcag gcgcctgtct tgccatcagg gatcaggcag gatcttgggc
      901 agagggtaca gcgaacccgg ttgtccgtct cgggatggaa ccagcgggcc ctatgggcgg
      961 gcatgggctt tctccacgat cgaggcgatg gaattgcggg ccctgtcagc caatgcctcg
     1021 ggtgtctcgt cggagccggg gatgatcggg gagagtatct tgagacagat ttttttgccg
     1081 aagcggggga aggtttttcc atagggcatg gcctcaaggg caccgtcgat ggcgacgggc
     1141 acaaccggga cacgggtccg gcaggcgatc atggcaaagc ccggcctgaa ggaggccagt
     1201 ccgccatccc ggctgcgcag tccctcggga aacaccatca ggttatagcc ggcctcgatg
     1261 acggccgccg aactgcgcag ggtggcggcc aggtcgctct tggcatgcac gacgatcaga
     1321 ttgcgtccac gggagaaaaa cttgacaacc cgttccataa cgttggaggc cttgatccag
     1381 gtgacggatc gggccaaaaa gcgtttgggc aatttggcga tcagggtcgg ggcatcgaga
     1441 tagctttcgt ggttggcgac gatgatgcac ggttctccgg ggagattttc cagaccctcg
     1501 atccgcaggt gaaagaggtg ccgcatgaaa aaggagagga gggcccgggc ggaactgcgg
     1561 gttcccttgc ggagtttcgg catttcgacg ggaccgtcaa agggatcgcc cgactggagg
     1621 acctcgtcgt ccgtggcagc ccgtgccagc gagactgctt ccgcgacggt ggcggcccgt
     1681 gccagatccg cctcggcgat tgtcaggccc agttcggact ggaggtaggc ggccatctcc
     1741 agccggtcga gggagtcgag tcccaaatca agatccagcc gggattccgg gcgagcctgg
     1801 agttttttcc ggcgactcaa gaaagcgcag acctgagcca gcgcactgtc ctggatggct
     1861 tcagtctttt cctgactggc acccggagca ctggtgaaat attctttaag ga
//
LOCUS       DAOSHE010000428         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_61058_length_1904_cov_3.359113, whole genome shotgun sequence.
ACCESSION   DAOSHE010000428 DAOSHE010000000
VERSION     DAOSHE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61058_length_1904_cov_3.359113"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLD82_09685"
     CDS             <1..277
                     /locus_tag="PLD82_09685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365952.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOG65692.1"
                     /translation="QVMNAAVRLFADAANARTKMENGFDLSDYDERTLRFAKEYSNSL
                     LAIDVNVDVDTMLDTGWKLFSRHFTQAEVGIKQELMQKYWPADSREA"
     gene            281..883
                     /locus_tag="PLD82_09690"
     CDS             281..883
                     /locus_tag="PLD82_09690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOG65693.1"
                     /translation="MAIRFQYNKTSLQDLGKQLKVRQRALPTLQNKESALRMEVKKAK
                     DQAAKINADFDAKLAELEGISRLWNEFDSSLLVVSDVRFGVKKIAGVKTPVLEEVVFA
                     ETSFPLFTAPAWYPDGLSLVRELAKLAFEREFFLRKMHLLDFARKKTTQKVNLYEKVQ
                     IPGFADAIRKIKRFLEDEDNLSKSAQKIVKSRARESEAAG"
     gene            880..>1904
                     /locus_tag="PLD82_09695"
     CDS             880..>1904
                     /locus_tag="PLD82_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65694.1"
                     /translation="MITPMRKYAFVVFHQDYAPLLRSLQDLGVVHVVEKKGVGDDEAV
                     RALTSRATRLSRAVRLLSQRSPVDDIQPASALGPDEILEELDRIQRESEQLEQARTVL
                     AKEIALVEPWGDIPTDILSRLSGAGITVRTFVVAEKKFNRDLLNDYPLAVVGEYGSRL
                     CLAAFLRSGEELPPIDGLEEVRLPHRGLSQALAEREEAEEKRQVLDLRLDALAAGTAG
                     DVLRAELSRVVAEADYARAELSAERDVEERVRILQGWVPRPAEAAVQAACDACGVVTI
                     AEDPQPGDDVPIQLKNGRFARLFEPITRLFSLPSYAELDLTGLFAPFFTLFFGFCLGD
                     AGYGLVI"
BASE COUNT          372 a          519 c          579 g          434 t
ORIGIN      
        1 ccaggtgatg aacgccgcgg tccgcctctt tgccgatgcg gccaatgccc gcaccaagat
       61 ggaaaacggc ttcgacctct cggattatga tgagcggacc ctgcgcttcg ccaaggagta
      121 ttccaactcc ctgctggcga tcgacgtgaa tgtcgacgtc gatacgatgc tcgataccgg
      181 ctggaagctc ttctcccggc actttaccca ggccgaggtc ggtatcaagc aggaactgat
      241 gcaaaaatac tggccggccg attcgcggga ggcttgatcc atggcgatac ggttccagta
      301 caacaagacc tcccttcagg acttgggcaa acagctcaag gtgcgccagc gggcgctgcc
      361 gaccctccag aacaaggagt cggccctgcg gatggaggtc aaaaaggcca aggatcaggc
      421 ggcaaagatc aatgccgact tcgatgccaa gctggccgag ctcgagggta tctcgcgcct
      481 ctggaacgaa ttcgactcca gtctgcttgt ggtcagtgat gtccgcttcg gggtgaagaa
      541 gatcgcgggt gtcaagactc cggttcttga agaggtggtg ttcgccgaaa cttccttccc
      601 cctctttacg gctccggcct ggtatcctga cggtctgtct ctcgtgcgtg aactggccaa
      661 gctggcgttt gagcgcgagt tcttcctgcg caagatgcat cttctggatt ttgcgcggaa
      721 aaaaaccacc caaaaggtca atctctacga aaaggtgcag attcccggtt ttgccgatgc
      781 cattcgcaag atcaaacgct tccttgagga tgaggacaat ctttccaaat cggcccagaa
      841 gatcgtcaag agccgggcca gggaatcgga ggctgccgga tgatcacacc tatgcggaag
      901 tatgcctttg tggtctttca tcaggactat gcgccgctct tgcgcagtct ccaggatctt
      961 ggggttgtcc atgttgtcga gaaaaagggc gtcggggatg atgaggcggt tcgtgccctg
     1021 acttcccggg cgacgcgcct cagtcgggcc gtgcgcctcc tgtcccaacg ctcaccggta
     1081 gatgatattc aaccggcgtc cgcccttggg ccggacgaaa ttctcgaaga gcttgaccgg
     1141 attcagcgtg agagcgagca gttggaacag gcccggacgg tcttggccaa ggagatcgcc
     1201 ctcgtcgagc cttggggtga tatccccacg gacattctgt cccggctttc cggtgctggt
     1261 atcacggtgc gcacctttgt ggtggctgag aagaaattca accgggacct gctgaatgac
     1321 tatccgctcg cggttgttgg tgagtatggc tcaagattgt gtttggctgc atttctccgg
     1381 agcggtgagg aactcccgcc gatcgatgga ttggaggagg tccgtttacc gcaccgtggg
     1441 ttgtcgcaag cactggctga acgagaggaa gccgaggaaa aacggcaggt tcttgatctc
     1501 aggctcgatg ctctggctgc cggaacagcc ggagatgtcc ttcgtgcaga actttcacga
     1561 gtggtggctg aggcggatta tgcccgggct gaactttccg ccgaacgtga tgtcgaagag
     1621 cgggtcagaa tccttcaagg ctgggtgccc cgtccggctg aagctgcggt tcaggcggcc
     1681 tgcgatgcct gcggtgttgt gacgattgcc gaggatcccc agcccggcga tgatgttccc
     1741 atccagttga aaaacgggcg ttttgcgagg ctctttgagc cgattacccg gctgttttca
     1801 cttccgagtt acgccgagct tgatctgaca ggcttgtttg ccccgttttt taccctgttt
     1861 ttcggttttt gtcttggtga cgccgggtac ggactggtca tcat
//
LOCUS       DAOSHE010000429         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_61190_length_1901_cov_3.997833, whole genome shotgun sequence.
ACCESSION   DAOSHE010000429 DAOSHE010000000
VERSION     DAOSHE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61190_length_1901_cov_3.997833"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLD82_09700"
     CDS             <1..809
                     /locus_tag="PLD82_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG65695.1"
                     /translation="DTGLLELILPELLEGFEVAQNEFHMHDIYYHNLYACDAAPKDKP
                     LVRFAALLHDIGKKRALDNAQRRTGSDDAIFHNHEVIGARMANAILRRLKFSNSDREQ
                     IVHLIKHHMFHYTDEWTDGAVRRLIRNVGIETLPDLFDLRRADRIGNGKKRGPSRTLE
                     KLQQRIQKILAEADAISVKDLAINGTDLMDSFKIPPGRVIGDCLTYLLERILDEPEKN
                     TREALLHEARMFFVDQGTLPPDALPAHPPREKPAKRSILGFIKRLFGRVG"
     gene            complement(989..1059)
                     /locus_tag="PLD82_09705"
     tRNA            complement(989..1059)
                     /locus_tag="PLD82_09705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Gly,seq:tcc)
     gene            complement(1114..1623)
                     /gene="nuoE"
                     /locus_tag="PLD82_09710"
     CDS             complement(1114..1623)
                     /gene="nuoE"
                     /locus_tag="PLD82_09710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG65696.1"
                     /translation="MSAQNTIERVFTLEKELQEFITDCRKKEHSESYLISVLHKVQAR
                     YGFLSRDHMQEVAELLGIPASTVSGVATFYHFFRLQPQGKYKVSFCMGTACYVKGAPG
                     IIDAFKEELGVDIGETTKDGLFSLEITRCLGVCGLAPVVMINETVHGKLTPASVPALI
                     AGIRKKEEG"
     gene            complement(1653..>1901)
                     /locus_tag="PLD82_09715"
     CDS             complement(1653..>1901)
                     /locus_tag="PLD82_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOG65697.1"
                     /translation="PGGCVGGGGQPYPHQGEYTIDFDLYRKRAGALYAIDKDKELRKS
                     HKNPHVLELYKDYLGEVGGHRAHELLHTHYQVRAPRGI"
BASE COUNT          415 a          603 c          513 g          370 t
ORIGIN      
        1 gggacaccgg gctcctggaa ctcatcctcc ccgaactcct tgagggcttt gaggtcgccc
       61 aaaacgagtt tcacatgcac gatatctact atcacaacct ctacgcgtgt gatgccgcgc
      121 ccaaggacaa gccccttgtg cgctttgcgg cactgctcca cgatattggc aagaagcggg
      181 ctctggacaa cgctcaacgc cgcacgggaa gcgatgatgc gatcttccac aaccacgaag
      241 tcatcggagc ccggatggcc aatgccatcc tccgccgtct caaattttcc aatagcgacc
      301 gggaacagat tgtccatctg atcaagcacc acatgttcca ctacacggac gagtggaccg
      361 atggtgcggt ccgccgtctg atccgaaacg tcgggatcga gacccttccg gacctgtttg
      421 acctgcgccg ggcggaccgg atcgggaacg gaaaaaaacg cgggccatcc cggacactgg
      481 agaagctgca gcagaggatc cagaaaatcc tggccgaggc cgatgccatc tcggtcaagg
      541 atctggccat caacggaacg gatctgatgg acagcttcaa gatcccaccg ggtcgcgtga
      601 tcggggactg cctgacctat ttgcttgagc ggattctgga cgaaccggaa aagaataccc
      661 gggaggccct cctgcacgaa gcccggatgt tctttgtcga ccagggcacg ctcccgccgg
      721 atgcacttcc tgcacatccg ccccgggaga aacccgcaaa gcggtccatc ctcgggttta
      781 tcaaacggct cttcgggcgg gtcggctgac ccccagcagg attccaccca ctgacagcca
      841 attcaccatc ccatcaggct cgcgccggag acacacccgg cttgagctcc cgtcggacca
      901 gtgtcggtga cccggaccgg tgccggacgc cattcatcca aaaaaaacaa gaacgcaggc
      961 cacaccatgg cgcaacctgc gttctttgag cgagtgaagg gagtcgaacc ctcgtcaaca
     1021 gcttggaagg ctgaggttct accgttgaac tacactcgca gacagctcaa agatacacac
     1081 tccagccggg aatcggcaag tctccgtgcg ggtttagccc tcttcttttt ttctgatccc
     1141 ggcgatcaat gcgggaacgg atgcgggcgt caatttgccg tgtacggtct cgttgatcat
     1201 cacaaccggt gccagaccgc agactccgag gcagcgggtg atctccaatg agaagagccc
     1261 gtccttggtg gtttcaccaa tgtccacacc cagctcttcc ttgaaagcat caatgatgcc
     1321 cggtgcgcct ttgacgtaac aggcggtccc catgcagaaa gagaccttgt acttgccctg
     1381 cggctgcagg cggaagaagt ggtaaaacgt ggccaccccg cttacggtcg aggccggaat
     1441 acccaacagc tcggccacct cctgcatatg gtcccgggaa agaaagccgt agcgggcctg
     1501 gaccttgtgc agcaccgaga tcaggtaact ctcggagtgc tcctttttcc ggcaatctgt
     1561 aatgaactcc tggagctcct tttccagggt aaaaacccgt tcaatcgtat tctgtgcgct
     1621 catcgcgtct tctccttgcc gtcttcccgg aatcaaatcc cccgcggtgc gcggacctga
     1681 tagtgcgtat gcaacagctc atgggcccga tgccccccga cctcacccag atagtccttg
     1741 tagagctcga gcacgtgcgg gtttttatgc gacttccgca attccttgtc cttgtcaatg
     1801 gcatacaggg cacccgcgcg cttccggtag aggtcaaagt cgatggtata ctcgccctgg
     1861 tgcggatagg gctggccacc gccaccaaca cacccccccg g
//
LOCUS       DAOSHE010000430         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_61223_length_1901_cov_2.356988, whole genome shotgun sequence.
ACCESSION   DAOSHE010000430 DAOSHE010000000
VERSION     DAOSHE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61223_length_1901_cov_2.356988"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1846
                     /locus_tag="PLD82_09720"
     CDS             248..1846
                     /locus_tag="PLD82_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG65698.1"
                     /translation="MTRHISCIVVLCFFVIVSCGTGKPDNRTFQGVLQGEPNYLNYVP
                     YSSAYEAQAVGLIYTPLFEIDMNATNALRPALATNITVSPDKRSLRVEMRQDAVWEDG
                     RPVTAHDVEYTWRMILDPASGAQNKISDLAEIESITVHNDWSFTALWKKPQVNSMLTI
                     AGMTPIPKHIWQGKDMRDPVLNRKPVGNGPWKFQEWKTGRHISFVTNTRWWGERKPFF
                     TRVLFRIIPEEGSIMAALKKGTLDLVDSLRAITWLDFVREDIKTNFGRLRSISTSYGV
                     IAWQQKGNPFFGDVLVRRAMTHCLDRASILKNIYRGVGQVQTGPFYRSSWAEDRSIAP
                     LAYDPALAARLLDEAGWKLDPAKKIRIKNGKEFRFEFLVTQGSENGRAIAVILQSELA
                     RIGVDMQVRTLEWSVFNKKLTAREFTAALFGWNNSVDCDVYDLWHSSMQNDGLNHIGY
                     ANPEVDRLLVEARGTFEMPERIRIAHRIHRLIHDDQPYTFLIANELLGLHTARMRGVQ
                     TSIRGAYSSWPGLVAWSAAGVRAE"
BASE COUNT          428 a          552 c          525 g          396 t
ORIGIN      
        1 gaaatgcagg ctctctacca aaaatacaaa aaataatccc ccgtcagttt gccccgacct
       61 cccgggcaac cccataccgg cttgtgctcc cgccggacca gtgccggata cccgggagac
      121 agcggacgcc atcacccaga aaaaatatca ggtcagcacg cgtggatcgt gcttgcgtgc
      181 ggcagtctga tggtttacaa tcttcaaaaa gaactactca tggcgtatgc gtcctctgga
      241 ggaagaagtg acccgacata tttcttgtat cgttgtattg tgttttttcg ttattgtctc
      301 ctgcggcaca ggaaaaccgg acaacaggac cttccaggga gtccttcagg gcgaacccaa
      361 ctacctcaac tatgtcccct attcgagcgc ctatgaagct caggccgtgg gcttgatcta
      421 cacacctttg ttcgagatcg acatgaatgc gaccaacgcc ttgcgcccgg cgctggcgac
      481 aaatatcacc gtcagtcccg acaagcgctc cctcagggtg gagatgcgtc aggatgcggt
      541 ctgggaggat gggcgaccgg tgaccgcgca cgatgtcgaa tacacttggc gcatgatcct
      601 cgacccggca tccggtgcgc aaaacaagat cagtgatctg gccgagatcg agtcgatcac
      661 tgtgcacaat gactggagct ttacggcact ctggaaaaag ccccaggtca actcgatgct
      721 gaccattgcc gggatgaccc cgattccaaa gcatatctgg cagggcaagg atatgcgtga
      781 cccggtcctc aaccggaagc ctgtcgggaa cggtccatgg aaattccagg aatggaaaac
      841 cggacgacat atttcattcg tgacaaatac ccgttggtgg ggcgaacgca agccgttttt
      901 tacccgggtc ctctttcgca ttattcccga agaaggctcg atcatggcgg ctctcaaaaa
      961 aggaacgctc gatctggtcg actcactgcg ggccatcacc tggctggact ttgtccgtga
     1021 agacataaaa acaaatttcg ggagactgcg cagtatcagc acctcctacg gcgtcatcgc
     1081 ctggcagcaa aagggcaatc cgttttttgg cgacgtcctg gtccggcgcg cgatgacgca
     1141 ttgtctcgac cgtgcatcca ttctcaaaaa catctaccgc ggagtcggtc aggtgcagac
     1201 agggccgttt taccggagtt cctgggcaga ggatcgttcc attgctccgc ttgcctatga
     1261 cccggcactc gcggcccggc tcctggatga ggctggctgg aagctggatc ccgccaaaaa
     1321 aatcaggatc aaaaacggga aggaattcag gtttgaattt cttgtcaccc aggggtccga
     1381 aaacggccgg gcaatcgcgg tcatcctgca gtccgaactt gcacgaatcg gggttgacat
     1441 gcaggtccgg acgctggagt ggtcggtctt caacaaaaag ctcacagctc gtgaattcac
     1501 ggccgccctg tttggctgga acaattccgt cgattgtgat gtctatgacc tctggcactc
     1561 ttcgatgcaa aacgacggcc tcaatcacat cggctacgcc aatcccgagg ttgaccggct
     1621 tttggtggaa gcacgcggca ccttcgagat gccggagcgg atcaggatcg cgcaccggat
     1681 ccaccggctg atccacgatg atcagcccta tacctttttg atcgccaacg aactcctcgg
     1741 gctgcacacg gcccggatgc gcggtgtgca gacttcgatc cgtggtgcct actcgtcctg
     1801 gcccgggctc gtcgcctgga gtgccgccgg cgtgcgggca gaataggaag acggtgtgct
     1861 gggatacatc ctgcgtcgcc tgggaatcgc catcccgacc c
//
LOCUS       DAOSHE010000431         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_62573_length_1875_cov_2.991758, whole genome shotgun sequence.
ACCESSION   DAOSHE010000431 DAOSHE010000000
VERSION     DAOSHE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62573_length_1875_cov_2.991758"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLD82_09725"
     CDS             complement(<1..817)
                     /locus_tag="PLD82_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG65699.1"
                     /translation="MRTVHDTLGSMEVPDDAFYGAQTARAIVNFPVSGRPLPPVLIRA
                     LVRIKRAWALTNGDHADPAKRPVFDAIVHAALSIEAGKYLEQFPVDVFQTGSGTSSNM
                     NANEVLASLASRLSGLSVHPNDDVNRCQSSNDVFPTAMQLALIDVSRERLLPALESLK
                     KSFDTLARENADVVKVGRTHLQDATPVLLGQEFSGYAAQLARSGRVIREGLQHLYELP
                     LGGTAVGTGLNAPAPLVAETIARLASWADVPLVEAADHFEAQSARDGIGLFSGA"
     gene            complement(1228..>1875)
                     /locus_tag="PLD82_09730"
     CDS             complement(1228..>1875)
                     /locus_tag="PLD82_09730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG65700.1"
                     /translation="SREEDIVCRYGGEEFVLLLPEVSASGTIHLLERVRESVHATQFF
                     CEHRITFSAGVASFPSHYEEDDDLSIVRAADRALYQAKFEGKDRTIEYFSEKRRHTRF
                     KQSWRISWERINPGQRDPASSETIITQNVSLGGVRFESGEWLTLDEDLLVGIRHGDSG
                     NDQEQTIRIPGRVIWTRRLSESRLAYGVAFGELSPEQSLFLGDALPVSRFLNDMP"
BASE COUNT          372 a          524 c          638 g          341 t
ORIGIN      
        1 aggcccccga gaacaggccg atcccgtcac gggcggactg ggcttcgaaa tgatcggccg
       61 cctcgaccag gggcacgtcc gcccacgaag ccagccgggc aatggtttcg gcgaccaggg
      121 gggccggtgc attcagaccg gtcccgacgg ccgttccccc aagggggagt tcgtagagat
      181 gctgcaagcc ctcgcggatg acgcgacccg agcgggcaag ctgtgcggcg tagcccgaga
      241 actcttggcc gagcaagacc ggggtcgcgt cctgaagatg ggtccggccc accttgacga
      301 cgtcggcatt ctcgcgggcc agggtgtcaa aggacttctt gagcgactcc agggccggga
      361 gaagccgctc cctgctgaca tcgatcaggg cgagctgcat ggcggtggga aaaacatcgt
      421 tggaagactg gcaacggtta acatcgtcat tgggatggac ggacagaccg gagagccggg
      481 aagccaggct ggccagaacc tcattggcat tcatgttcga ggatgtcccg gagccggtct
      541 ggaagacatc gacgggaaat tgctcgagat atttgcccgc ttcgatcgag agggccgcat
      601 ggacgatggc gtcaaagacc gggcgcttgg ccggatcggc atggtcgccg tttgtgaggg
      661 cccaggcccg cttgatgcgg acgagtgccc ggatcaggac gggcgggagc ggtcgtccgc
      721 tgacgggaaa attgacgatc gcgcgggccg tctgtgcccc gtagaaggca tcatcaggga
      781 cttccatgga tccgagggta tcgtgaactg tgcgcactac ttcttctccc gtcttgtgtt
      841 ctgcaccgag atgggcagtc tatcatagtt cccggcagta cacaagggcg ggcagggcag
      901 gttggtgttg cgacccggca aacgtctgca tgatgcgtaa aatttgggca gtcgcacaat
      961 atcccttcgg gatcttcgcc gggattgcac tcgggtcctt cgacatcgag gagggcgaca
     1021 caggcggtgc gccagccggg gctgagaatg tgcaggatac ggggcaagct ggcgactcga
     1081 acccattgtg aatgtgcaag ccagccccgc cggaccagtg gcagggtagc ggaaaaattc
     1141 aggacgccat acccaaaaac actccaaaag gaccagcgag ccattcccta tcccgggagc
     1201 ttgtgactgt ggctggtcgg cactgcgtta tggcatgtca ttgagaaaac gggagacggg
     1261 gagggcgtca ccgaggaaga ggctctgttc gggcgagagt tccccgaagg cgactccgta
     1321 ggccaggcgt gactccgaga gacgccgtgt ccagatgacc cgccccggga tgcggattgt
     1381 ctgttcctga tcattgccgg aatcaccgtg acggataccc accaggagat cttcgtccag
     1441 ggtgagccac tccccgcttt cgaagcggac accgcccagg gagacgtttt gggtgatgat
     1501 ggtctcgctg ctggcaggat cgcgttggcc cggattgatt cgttcccagg agatgcgcca
     1561 gctttgcttg aagcgggtgt gacggcgttt ttcggaaaag tattcgatcg tgcggtcctt
     1621 gccctcgaac tttgcctggt agagtgcccg gtccgcggcg cggacgatgg agagatcgtc
     1681 atcctcctcg tagtgcgagg ggaaggaggc gactccggca ctgaaggtga tcctgtgttc
     1741 acagaagaac tgggtggcat gcacggattc gcggacccgc tccagaagat ggatcgttcc
     1801 ggaggcactg acctcgggaa ggaggaggac gaactcctct ccgccgtagc ggcagacaat
     1861 gtcctcttcc cggct
//
LOCUS       DAOSHE010000432         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_63589_length_1855_cov_4.442222, whole genome shotgun sequence.
ACCESSION   DAOSHE010000432 DAOSHE010000000
VERSION     DAOSHE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63589_length_1855_cov_4.442222"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLD82_09735"
     CDS             complement(<1..853)
                     /locus_tag="PLD82_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65701.1"
                     /translation="MVRMTWLSIVFLLLPVAGFSAGEWPADWLRPHIGTNGGGYSQNE
                     GARLARLSPWKMTPRGNEVFRIIRGLVPSEMNPGDIVWLARKLGSPLGQADRFQLWKR
                     LRAISSDESLVSSYSNAVYGDGETPYNIMPQEPYGWYDFNRDGKVDLFLATQGYFGPS
                     YGYVAIALDTVPARYLLDISSGLSVVKREGLRTILRFDVMQIDQAEAQIEYFIIADNR
                     TGRISYGPCLAYASETVMPQSWLGRSRVLRAPTLRIRKGPVVDNTAVNPDEAWKLEGT
                     SVLAGNIV"
     gene            complement(921..>1855)
                     /locus_tag="PLD82_09740"
     CDS             complement(921..>1855)
                     /locus_tag="PLD82_09740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOG65702.1"
                     /translation="EPLVEIQQRIDELGSFSTTEGVDFTAEIAKLRDKSESMLKEIYS
                     KLTPWQIVQVARHPQRPYFLDYIRMMSDDFEEIHGDRLFGDDKAIVTGFATISGVRMA
                     VIGQQKGRDVKENITRNFGMPRPEGYRKALRVMDLAARFNLPIVTFIDTPGAYPGLDA
                     EERGQGEAIARNLREMALYEVPIVVVVIGEGGSGGALAIGVGDRVLMMQNSIYSIISP
                     EGCASILWRDATKAEDAANALRITARDLVELGIVDEIVPEPLGGAHREPMQTAMTLKE
                     AILRSLADLQKQPKQRLVERRFEKIRRIGPVLEQ"
BASE COUNT          405 a          572 c          509 g          369 t
ORIGIN      
        1 ctacgatatt ccccgcaaga accgacgttc cctcgagttt ccaggcttca tcgggattga
       61 cagcggtatt gtccacaacc ggtcccttgc gaatccggag cgtaggcgca cgcagcacgc
      121 gtgaccgtcc gagccagctc tgtggcatga ccgtctcgga agcgtacgcc agacagggcc
      181 cgtacgaaat ccgcccggtc ctgttatcgg caataatgaa gtactcgatc tgcgcctctg
      241 cctgatcgat ctgcatcaca tcaaaccgga ggatggtccg cagcccctcg cgcttgacga
      301 ccgacaaccc gctggagata tccagaaggt atctggcagg aacagtgtca agagcaatcg
      361 ccacatatcc gtaggatgga ccaaagtatc cctgggttgc caaaaagagg tcaaccttgc
      421 cgtccctgtt gaaatcatac cagccatagg gctcctgggg catgatgttg tacggtgtct
      481 cgccatcacc atagacggca ttgctgtagc tcgagacaag tgactcgtca ctggaaatgg
      541 cacgcagacg cttccagagc tggaagcgat cggcctgccc cagaggggat ccaagctttc
      601 gggccagcca gacgatatcc ccgggattca tctccgaggg gacaaggccc ctgatgatcc
      661 ggaaaacctc attccctcgt ggagtcatct tccagggaga gagccgggcc agtctggccc
      721 cttcgttttg tgaatagccc ccgccattgg tcccgatatg ggggcgaagc cagtccgccg
      781 gccactcccc ggcagagaat cccgcaacgg ggagaagcaa aaacacaatg ctcagccatg
      841 tcatgcgaac cattccaaac ctcctgaaaa ccggtacgat atcgctcttg cgacaccggg
      901 aacggaaggt tgtcagtccc tcactgctcg agaacggggc caatccggcg gatcttctcg
      961 aaccgacgtt cgacaagccg ctgcttgggc tgtttctgaa gatcggccag cgagcgaaga
     1021 atcgcttcct tgagcgtcat ggcagtctgc atcggctcac gatgggcccc gcccaatggc
     1081 tcgggaacga tctcgtccac aatacccagt tcgaccagat ccctagccgt gatgcgcagg
     1141 gcgttggcag catcctcggc cttggttgca tcccgccaga gaatggacgc gcacccctcg
     1201 ggggagatga tcgagtagat cgagttttgc atcatcaaga cacggtcgcc gactccgatc
     1261 gccagagcgc cgccggaccc cccctcaccg atcacgacga ccacaatcgg aacttcatag
     1321 agcgccatct cgcgcagatt gcgggcaatc gcctcgccct gcccgcgctc ctcggcatcg
     1381 aggccgggat aggcccccgg ggtgtcaatg aaggtcacga tgggaaggtt gaagcgggcc
     1441 gcgaggtcca tgacgcgcag ggccttgcgg tagccttcag gccggggcat tccaaaattt
     1501 cttgtaatgt tttctttgac atcgcggccc ttctgctgcc cgatgaccgc catccgcacc
     1561 ccgctgatgg tggcaaaacc ggttacaatc gccttgtcgt ctccaaaaag ccggtcacca
     1621 tggatctctt cgaaatcgtc actcatcatc cggatataat caaggaaata gggccgctgg
     1681 ggatgtctgg caacctggac gatctgccag ggtgtgagct ttgaatagat ctccttgagc
     1741 atcgattcgc tcttgtcccg gagcttcgca atctcggcgg taaaatccac accctctgtt
     1801 gttgaaaacg aaccaagttc gtcgatacgc tgctggattt ctacaagggg ttcct
//
LOCUS       DAOSHE010000433         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_63648_length_1854_cov_2.770428, whole genome shotgun sequence.
ACCESSION   DAOSHE010000433 DAOSHE010000000
VERSION     DAOSHE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63648_length_1854_cov_2.770428"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLD82_09745"
     CDS             complement(<1..451)
                     /locus_tag="PLD82_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65703.1"
                     /translation="MPRLTLFELWLVFAALCALASWISYRRRERARWPQVLFSFWGTL
                     SGALVSRVGLVVLAGGALATLGLVTSGLDRELQIWFQERNPLGQELARAFLVGGNFWH
                     PMAGVLLLLWARLRNRRVQFAAAAAALQAIIATGLVVNALKWMTGRRG"
     gene            complement(451..684)
                     /locus_tag="PLD82_09750"
     CDS             complement(451..684)
                     /locus_tag="PLD82_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65704.1"
                     /translation="MKQHGVCPKCGSREIALSSGSNTSDVNTILTGMTYLSAVACEKY
                     ICLGCGYTEQYVRDQAGLEKISRKLPRHRVEGT"
     gene            complement(719..1450)
                     /locus_tag="PLD82_09755"
     CDS             complement(719..1450)
                     /locus_tag="PLD82_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOG65705.1"
                     /translation="MTGWKPLIVDEDNHILVVVKPANLPAQADASGDPDLLSILKEHI
                     RQRDKKPGNVYLGLVHRLDRPTSGLMVFAKTSKAAARLARALQEDRMTKEYLAVLRGI
                     APGAADGGWTRWTDWLVKDGASNTVRVADKGTAGAKEAILTLQCVAVSGGLSLVRVRL
                     ETGRSHQIRVQAASRGLPLWGDMRYDEAHNRAGMQLALCACRLVFPHPVREAECSYSI
                     FPPTEEPWNRFSIPDGGISETAQQI"
     gene            1602..>1854
                     /locus_tag="PLD82_09760"
     CDS             1602..>1854
                     /locus_tag="PLD82_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG65706.1"
                     /translation="MATNMTKDMFLKDVFDYENKKEWEFQGELPCVIDFYADWCGPCK
                     MIAPVIEELSKEYAGKVNFYKINTESEMELAGAFGIQSIP"
BASE COUNT          387 a          567 c          529 g          371 t
ORIGIN      
        1 gcccacgacg tccggtcatc cacttgaggg cgttgacaac aagcccggtc gcgatgatcg
       61 cctgcagggc ggcggccgcc gccgcaaact ggacacgcct gttgcgcagt cgggcccaga
      121 ggagcaggag aactccggcc atcgggtgcc agaaatttcc gcccaccaaa aatgctctgg
      181 cgagttcctg gcccaggggg ttgcgctcct gaaaccagat ctgcagttcg cggtccagtc
      241 cgctcgtcac caggcccagt gtggccaggg ctccgcctgc cagcaccacc aggccaactc
      301 gcgacaccaa cgcgccggag agtgttcccc aaaaagaaaa caacacctgg ggccagcggg
      361 ccctttcacg gcgacggtac gagatccatg aggccagcgc gcagagggca gcaaagacca
      421 gccagagctc gaacagggtc aagcggggca tcaggtacct tccacccggt gcctgggcaa
      481 cttgcgggag attttttcga gaccggcttg gtcccggaca tattgttcgg tatacccgca
      541 acccagacag atgtactttt cacaggccac cgcactgagg taggtcatcc cggtcaggat
      601 ggtgttgaca tcggaggtgt tggatccgct ggagagagcg atctcccgtg atccgcattt
      661 gggacagact ccgtgctgtt tcatggcttg ccactcctca acaagattgc atcacgcatc
      721 atatttgttg cgctgtttcg gatataccac cgtccggtat tgaaaacctg ttccatggtt
      781 cctctgtggg tggaaagatg ctgtaggaac actcggcctc ccgtaccggg tgcggaaaca
      841 cgagtcggca ggcgcagagc gcgagttgca tgccggcgcg gttgtgcgct tcatcatagc
      901 gcatgtcacc ccagagcggc aatccccgcg aggccgcctg gaccctgatc tgatggctcc
      961 gaccggtttc gaggcgcacc cgcaccagcg acaatccgcc ggaaacggcg acgcattgca
     1021 gcgtgaggat cgcttccttg gccccggccg tccccttgtc cgccacccgg accgtattgc
     1081 tcgcaccatc cttgacaagc cagtccgtcc aacgtgtcca cccgccgtcg gctgcgccgg
     1141 gggcaatccc ccgcaggacc gccaggtatt ccttggtcat ccggtcttcc tgcagcgcgc
     1201 gggccagccg ggcggcggcc ttgcttgtct tggcaaagac catcagcccc gaggtcggcc
     1261 ggtcgagtcg gtgcaccagc ccgaggtaga cgttgcccgg cttcttgtcc cgctggcgga
     1321 tatgctcctt gaggatggac aaaagatctg ggtcacccga ggcatcggcc tgggccggca
     1381 ggttggccgg cttgacgacc acgagaatgt ggttgtcctc atccacaatc agcggcttcc
     1441 atcccgtcat ttccatctcc ctaaaatact ctttcggtcc ggatccggct cgcccattgt
     1501 cagtgacagg gccagatcct cgctgatttt ttttaaaaaa ctgttgattt ccggattctt
     1561 atatagtatt ttttttatat ataacaagca aggagacagc catggcaacc aatatgacca
     1621 aagatatgtt cctcaaggac gtatttgact acgaaaacaa gaaggaatgg gagtttcagg
     1681 gtgagctgcc ctgcgtgatc gatttttacg ctgactggtg cggaccatgc aagatgatcg
     1741 cccccgtcat cgaagaactc tcgaaagaat acgccgggaa ggtcaatttt tacaagatca
     1801 acaccgagag cgaaatggaa ctggcgggcg ccttcggaat ccagagcatt ccca
//
LOCUS       DAOSHE010000434         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_63779_length_1851_cov_3.258352, whole genome shotgun sequence.
ACCESSION   DAOSHE010000434 DAOSHE010000000
VERSION     DAOSHE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63779_length_1851_cov_3.258352"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLD82_09765"
     CDS             <1..225
                     /locus_tag="PLD82_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65707.1"
                     /translation="PGEMAENEHNLESGGRLFSRYQTPFGELWIITTADRSETYMCFP
                     TQYLEWATGGQVGNTEARHRQPPNEGQSKP"
     gene            complement(557..901)
                     /locus_tag="PLD82_09770"
     CDS             complement(557..901)
                     /locus_tag="PLD82_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65708.1"
                     /translation="MKTGATVTSHGPMNHSVNPATGKMYWVSYWGAGTGPGYFKMADS
                     PSGSNAKIIEANVRAENHADYMAHDPVAIRVWYFHRTSTGVTLKYANGDDTGVTGTGI
                     TGFDSPLVIFNE"
     gene            complement(1381..>1851)
                     /locus_tag="PLD82_09775"
     CDS             complement(1381..>1851)
                     /locus_tag="PLD82_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65709.1"
                     /translation="PVKGKSFSIHTSRKPTKVQLIVRGERLPGYQTSERPGLHLQREQ
                     ARDVMSRFRQALASGRTTGLERYLPAHGILYKSGKSVSRLTPAEALSRLSLRSSKSPN
                     MRQSGSRILIDISGPDSTQRWTIEEAAGNWRVTKIEDLNFVLPGANLTRNPQRL"
BASE COUNT          416 a          512 c          535 g          388 t
ORIGIN      
        1 ccgggcgaaa tggccgaaaa cgaacacaac ctcgagtccg gggggcggct cttcagccgc
       61 taccaaaccc catttggcga gctctggatc atcacgaccg ccgaccgcag cgaaacctat
      121 atgtgcttcc cgacacagta cctcgaatgg gccaccggcg gacaggttgg aaacaccgag
      181 gccaggcacc gccagccacc caacgaaggc cagtccaaac cctgattctc ctgccctgaa
      241 cttccgttcc gacgtacagc ctgcacagac acaatcaagt ccaaaatctt tttgaggaac
      301 agcctcgggc cggataccct gcggcagacc cagggaatgg tctgtctgca ggtgagcggg
      361 gacaaacaac cggccgaata caaaaggaaa ccatttgtcc tgacgaaact gccatgaggg
      421 gttaaccccg atgggacaaa aatccggttc actacagtca cgcgatctgc cagcacccga
      481 atacaaaacg ccgagcaggg ggacagttcc cgtggccggc gttcttgcgt tccaccctga
      541 aaaataagcg aggggactat tcgttgaaga tgaccaacgg cgaatcgaag ccggtgatgc
      601 cggtcccggt cacgccggtg tcgtcaccgt tggcgtattt cagggtcacg cccgtggatg
      661 tgcggtggaa gtaccaaaca cggatggcca cgggatcgtg ggccatgtag tcggcatgat
      721 tttcggctcg gacattggcc tcgatgatct tcgcattgct tccggaaggg gaatcggcca
      781 tcttgaagta ccccggccct gtccccgcgc cccagtagga aacccagtac atcttgccgg
      841 tagccggatt gacggaatgg ttcatcggac cgtggctggt tacggtcgcg cctgtcttca
      901 cgatgtcgct ctccgacccg ccgctcaggc cgatggaata ctatttgtcg atagctccgt
      961 tcacccaaac cagtcggtca ttcgtcatcc gagcaggccg tcacgacaaa agccatcatg
     1021 gaaaccagta ctgctgccca tactttcatc atgtttttcc tccaagggtc ccgtaccgtc
     1081 aaccgcgaaa ttgcagattg ggcctatcat tcaggtagag atacgattcc attttagaaa
     1141 tgacatcacc acggaggggg attttacggg atttgacaga atctctgaaa tttggaacaa
     1201 ttttttttct gcatcaagag agacaggggc gggcattcca aagacagtat tgatcgctcg
     1261 tccccagaag agctgattat ggagaaccac gttgtttttg ttcaaacggt tcaacatgag
     1321 agagtctccg tcgtacggga acggagggat tcctgcagga tgatgatgga caagacctgt
     1381 ctacaggcgt tgcggattcc gggtcaggtt ggcgccgggg aggacaaaat tcaagtcttc
     1441 gatttttgtg acacgccagt tgccggcagc ttcttcgatc gtccagcgtt gggtgctgtc
     1501 cgggcctgaa atatcaatca ggatcctgga gccgctttgc cgcatattcg gtgatttcga
     1561 ggaacggaga gagagacggg acagcgcctc ggctggtgtg agccgggaga cggattttcc
     1621 cgacttgtac aggataccgt gggcggggag atagcgctcc agtccggttg tgcggcctga
     1681 tgcgagggcc tgcctgaagc ggctcatgac atcccgggct tgttcgcgtt gcagatggag
     1741 gccgggtcgc tccgaagtct ggtacccggg gagccgttca ccacggacga tcaactgcac
     1801 cttggtcggc ttgcggcttg tatggatgga gaagctcttt cccttgacgg g
//
LOCUS       DAOSHE010000435         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_64566_length_1836_cov_3.766985, whole genome shotgun sequence.
ACCESSION   DAOSHE010000435 DAOSHE010000000
VERSION     DAOSHE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64566_length_1836_cov_3.766985"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLD82_09780"
     CDS             <1..525
                     /locus_tag="PLD82_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG65710.1"
                     /translation="FLGKLPIDPSVASSGDDGKPFIYHKSDSPAARAFQTVLRPILDL
                     LTTIHRSENVHQKKETSMKRFAIPTASGRLCSHFGHCEAFAIIEADPESKTIGTRNDV
                     VPPPHEPGVLPKWLGEQGVNVIIAGGMGQRAQELFTAQGIAVCVGAPADEPEALVAAW
                     LNKTLVTGANACDH"
     gene            619..1026
                     /locus_tag="PLD82_09785"
     CDS             619..1026
                     /locus_tag="PLD82_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG65711.1"
                     /translation="MERTYKRSRQRERILETLMQTTTHPTADWVYARLKPDFPDLSLG
                     TVYRNLNILVEQDLVRRIDNGSSFDRYDARRDPHYHFVCEQCGKIIDLGIPVDTALED
                     RVSGETGLVIKRHKIDFFGLCQDCQTQSGIRPD"
     gene            1141..>1836
                     /locus_tag="PLD82_09790"
     CDS             1141..>1836
                     /locus_tag="PLD82_09790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG65712.1"
                     /translation="MENTQNVVSMPRIGEKAPAFKAVTTQGEINFPEQYAGSWVILFS
                     HPADFTPVCTSEFMTFASLEPQFEALNCKLVGLSVDGLYSHIAWLRTIKDKIEYKGMK
                     NIEVKFPLIEDITMEVAKKYGMIQPGESSTKAVRAVFVIDPNGVIRTIIYYPLSMGRN
                     FDELVRVLQALQTADAFSIATPADWRPGDEVIVPTAGSCGVAKERMENSKDMKCYDWF
                     FCTKQIDKDTVMKA"
BASE COUNT          440 a          521 c          460 g          415 t
ORIGIN      
        1 tttctgggta aactgccgat cgacccaagc gttgcgagtt ctggcgacga cggaaaaccc
       61 tttatctacc acaagtctga ctcaccggca gcccgcgcct tccagactgt cctgcgcccc
      121 attctcgatc tgctcaccac aattcaccga tccgagaatg ttcaccaaaa aaaggagact
      181 tcaatgaaac gttttgccat ccccaccgct tcaggccggc tctgttccca ctttggccat
      241 tgtgaggcct ttgccatcat cgaggcggat cctgagtcaa aaacaatcgg tacacgcaat
      301 gatgttgtcc cgcctcctca cgaaccgggt gtgctgccca aatggctggg cgaacagggt
      361 gtcaacgtca tcatcgccgg cgggatggga cagcgggccc aggaactctt tacggcccag
      421 ggcattgctg tgtgcgtcgg tgccccggcg gatgagccgg aagccctggt tgccgcctgg
      481 ttgaacaaga cccttgttac cggcgcaaat gcctgcgatc attgagcaaa aaccatgttt
      541 tttccgctcc gttttcttga cacgttcacc aacctagctt atattaagaa tagttccgat
      601 ttttaaaaag gatcaggcat ggagcgtaca tacaagcgga gcagacagcg tgagcggatt
      661 ttggaaaccc tgatgcagac cactacccat ccaaccgcag actgggtcta tgcccgcctc
      721 aaacccgact ttcccgacct gagcctgggg accgtctacc gcaatctcaa tatcctggtc
      781 gagcaggatc ttgtcagacg gatcgacaat ggaagctcgt ttgaccgcta tgatgcccgg
      841 cgggatcccc actaccactt cgtctgtgag cagtgcggga agatcattga ccttggcatt
      901 ccggtggata ccgcgctgga ggatcgggtc tccggcgaaa ccgggcttgt gatcaagcgg
      961 cacaagattg atttttttgg cctctgtcag gattgccaga cacagtcagg aataagacca
     1021 gactgaattt tttggaacag ccgcatccgc ccttggtggg tattgttctt tctataatat
     1081 tgcaaatcgt tacgatatgc aaaaagggta cgcccttatc aagaaaagga gataatcatt
     1141 atggaaaaca ctcaaaacgt tgtcagtatg ccccgcatcg gtgaaaaagc ccctgcattc
     1201 aaggctgtca ccactcaggg agaaatcaat ttccccgaac aatatgccgg aagttgggtc
     1261 atcctcttca gccacccggc cgatttcacc ccggtctgca cctccgagtt catgaccttt
     1321 gcctcgctgg agccgcagtt tgaggccctc aactgcaagc tggtcggtct ctccgttgat
     1381 ggcctctaca gccatatcgc ttggttgcgc accatcaagg acaagatcga gtacaaggga
     1441 atgaaaaata tcgaagtcaa attcccgttg atcgaagaca tcaccatgga agtcgccaaa
     1501 aagtacggca tgatccagcc cggtgaaagc tcgaccaagg ccgtacgtgc tgtgtttgtg
     1561 attgatccca atggcgtcat ccggactatt atctactatc cactctccat gggccggaat
     1621 tttgacgagc tggtccgcgt tcttcaggcg ctccagaccg ccgacgcgtt ctctattgca
     1681 acacccgccg actggcgccc cggtgacgag gtcattgtac cgacggccgg ctcttgtggt
     1741 gtcgccaaag aacgcatgga gaactccaag gacatgaaat gctatgactg gttcttctgc
     1801 accaaacaga tagacaagga cacggtgatg aaagcc
//
LOCUS       DAOSHE010000436         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_64590_length_1836_cov_2.570466, whole genome shotgun sequence.
ACCESSION   DAOSHE010000436 DAOSHE010000000
VERSION     DAOSHE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64590_length_1836_cov_2.570466"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1836
                     /locus_tag="PLD82_09795"
     CDS             18..>1836
                     /locus_tag="PLD82_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65713.1"
                     /translation="MDVSVILVYDQDTVEDNPLADLEQVLVPSRELTNLRLFAMAIDA
                     IPDMIGILDTAYTVVCYNKAVYKMLGVQPERVIGNKCYANVGRQEICEVCAVKECYKS
                     HAIEIVERYDPALRMWIELRAYPVFDSDGQLVNVIEHFRDITQRKLSEKALIEQKQLL
                     QSITDNVFDLIALTDMQGHFLFLGKSHAILGYDLDWLIGRHVMEFVHPEDVERISGEF
                     AGFLEQRETIPRVEYRYRCADGQYIWLETVGRAIPSASGGEGRLLFSSRDVTARRRAE
                     QDLAESEQRFKALHNASFGGITIHDKGVILECNQGLSQISGFSMDELIGMDGLLLIAE
                     DWRPLVRSNIQAGYERPYEAVGVRKDGTRYPLRLEARNIPYRGRQVRVVEFRDITEQK
                     QAEEHGRMLEAQLRQEQKLSAVGTLASGVAHEINNPLTGIINFAQLILDRLPGNVETI
                     RDWASEILAEGDRIRTIVSNLLLFSRQESGQRQAVSPGSLVGAILSLCGNLLKKHEVE
                     VEVHLDDDLPDLDCVQGQIQQVLLNLIINARDALDDHYPGFHAEKKVFITGHNLHDGE
                     GRWVRFIVEDHGGGMSDAVAARCMEPFFTTKLGDRGTGLG"
BASE COUNT          432 a          432 c          546 g          426 t
ORIGIN      
        1 cggagaagtg cacgggtatg gatgtatccg taattctggt gtatgatcaa gacactgtgg
       61 aagacaatcc actggcagat cttgaacagg tcttggttcc ttcgagagag ctcacaaacc
      121 tgagactctt tgccatggcc atcgatgcga ttccggacat gatcggcatc ctggacacgg
      181 cctataccgt tgtctgctac aacaaagcag tctacaagat gctgggagtc cagcctgagc
      241 gagttattgg gaacaagtgt tacgccaatg tcggtcggca ggagatctgc gaagtctgtg
      301 ctgtcaaaga atgctacaag agccatgcaa tcgaaatagt ggagcgatat gatccggctc
      361 tccggatgtg gatcgagctt cgtgcctatc ccgttttcga cagtgacggg cagttggtca
      421 atgtgattga gcattttcgc gatattacac aacgcaaatt aagcgaaaaa gccctgatcg
      481 agcaaaagca gcttttgcaa agcattacgg acaatgtttt tgacctgata gccttgacgg
      541 acatgcaggg gcattttttg tttctcggga aatcgcacgc catactcggg tacgatctgg
      601 actggttgat cgggcgacac gtcatggaat ttgtgcatcc tgaagacgtt gagcggatat
      661 ctggagagtt cgccggtttt ctggaacaaa gagaaacaat tccgcgggtt gagtaccggt
      721 acaggtgtgc tgacgggcag tatatctggc tggaaacggt cgggcgggcc attccgtcgg
      781 catcgggagg agagggacgg ctcctgttca gttcgagaga tgtgacagcg cgcaggcggg
      841 ccgaacagga tctggccgag agcgaacaac ggttcaaagc acttcacaat gcttcctttg
      901 gtggaattac gatccatgac aagggtgtga ttcttgagtg caatcaaggt ctttcccaga
      961 tctcaggttt ttccatggat gaattgatcg gcatggacgg cttgttgctg attgccgagg
     1021 attggcgccc gctggttcgg agcaatattc aggccgggta tgaacgcccg tacgaggctg
     1081 tcggtgtccg caaggacggg acacgctatc cgctccgtct ggaagcgcgc aatattccct
     1141 atcggggaag acaggtgcgc gtggtcgagt tcagggatat tacggaacaa aaacaggctg
     1201 aagaacacgg ccgtatgctt gaagcccagc tccgccagga gcaaaagctc tccgcggtgg
     1261 ggacgttggc cagcggggtt gcgcacgaga tcaataatcc gctgaccggg atcatcaatt
     1321 tcgctcaatt gattctggat cgcttgcccg gcaacgtgga gacaatccgg gattgggcca
     1381 gtgaaatttt ggcagaaggt gaccggatcc gcacaattgt cagcaacctc ctgctctttt
     1441 cgagacagga gtccgggcaa agacaggcag tctcccccgg atcactggtc ggggccatcc
     1501 tttcgctctg cggcaatctc ctgaaaaagc atgaggttga agtcgaagtc catctggatg
     1561 atgatttgcc cgatcttgat tgtgtgcaag gacagatcca gcaggtcctg ctcaatctga
     1621 tcatcaacgc acgtgatgct ctggatgatc actatccagg gtttcatgca gaaaaaaaag
     1681 tgtttatcac cggacacaat ctgcatgacg gtgaaggccg ttgggtgcgt tttattgtcg
     1741 aggatcatgg cggggggatg tcggatgcgg tcgcggcccg ttgtatggaa ccgtttttta
     1801 ccaccaagct cggggatcgc ggaaccggtc tcggcc
//
LOCUS       DAOSHE010000437         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_64946_length_1829_cov_3.531003, whole genome shotgun sequence.
ACCESSION   DAOSHE010000437 DAOSHE010000000
VERSION     DAOSHE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64946_length_1829_cov_3.531003"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLD82_09800"
     CDS             <1..291
                     /locus_tag="PLD82_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65714.1"
                     /translation="VFFRLLQKKDIVIYEGHLPFDDIKPEDGEILPEFLKRALAYFLA
                     HGLIDIRIDMKVAFCGDRRVLDDIHVLAEHGYGEDRYGNDVMLPPPLAYLKR"
     gene            complement(303..1073)
                     /locus_tag="PLD82_09805"
     CDS             complement(303..1073)
                     /locus_tag="PLD82_09805"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOG65715.1"
                     /translation="MPTKKEIVEDWLPRYTGAPLSSFTNYILLTNFINYVEMFAEIRG
                     VEILGRNKPMQMATDGELTIINFGMGSPLAATVMDLLSAVHPQAVLFLGKCGGLKKTQ
                     LGDFILPIAAIRGEGTSNDYLPPEIPALPSFRLHKAVSIEIRQRNLEYWTGTVYTTNR
                     RVWEHDKDFRTYLTKARAIGIDMETATLFTVGFANEIPRGALLLVSDNPMVPEGIKTS
                     KSDTSVTAKFVRTHLEIGMESLRNLSKTGQSVKHMRFE"
     gene            complement(1179..1661)
                     /locus_tag="PLD82_09810"
     CDS             complement(1179..1661)
                     /locus_tag="PLD82_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOG65716.1"
                     /translation="MQIRSHGMLPMLLLLCLTGCMTTVSRAQGTNTPTGAVQPAGKRG
                     DVVTIARRYLGTPYRYGGSDPRGFDCSGFVLYVFGQVGVRLPHTAAGQFGQLQKIQTP
                     QPGDLVFFHTYGAGVSHVGIYAGNNQFIHSPRAGKTVEYTDMRIEYWRTRYRGARRAL
                     "
BASE COUNT          388 a          505 c          523 g          413 t
ORIGIN      
        1 gtctttttca gactgctgca aaaaaaggac atcgtcatct atgaggggca tcttcccttt
       61 gatgatatca agcccgagga tggcgaaatc ctgccggagt ttctcaagcg ggcgctggcc
      121 tatttcctgg ctcacgggct gattgatatc cggatcgaca tgaaagtggc cttttgcgga
      181 gacaggcggg tactggacga catccacgtc ctggccgaac acgggtacgg cgaggatcgc
      241 tatggcaatg atgtcatgct gcccccgccg ctggcctatc tcaaacgctg aagattcggc
      301 aattattcaa accgcatgtg cttgacggat tgtccggtct tggagaggtt gcgcagggac
      361 tccatcccga tctcgaggtg tgtccggaca aatttggccg taactgacgt gtcgctcttg
      421 ctcgtcttga tgccctcggg caccatgggg ttatccgaca ccaggagcaa ggctccccgc
      481 ggaatctcgt tggcaaaacc gaccgtaaag agtgtcgcgg tttccatgtc gataccgatc
      541 gcccgggctt ttgtcagata ggtccggaaa tccttgtcat gctcccagac acgacgattg
      601 gtggtgtaga cggttcccgt ccagtactcg agattccgct gcctgatctc gatcgagacg
      661 gctttgtgaa gacggaatga cgggagcgcc ggaatctcgg gtggcaggta gtcattgctg
      721 gtcccttcac cccggatggc cgcgatggga aggataaaat ctccgagctg tgtttttttg
      781 agtccaccac actttccgag aaacaggacc gcctggggat ggacggcgga caaaagatcc
      841 atcacggtgg ccgccagcgg actgcccatc ccgaagttga tgatcgtcag ttctccgtcg
      901 gtggccatct gcatcggctt gttgcggccg aggatttcga cgccgcggat ctcggcgaac
      961 atctccacat agttgataaa atttgtcagg aggatatagt tggtgaaact cgacaatggc
     1021 gcacctgtat accgggggag ccagtcctca acgatttcct tttttgtcgg catgctcttt
     1081 ccgcccgtca tttccggcct ccatcctctg atgggccaag tatagcactc gatgatgccg
     1141 gtcatccaca ggaagaagtc cggggaaaga cgcgcgtctt acagcgccct ccgggcaccc
     1201 ctgtagcggg tgcgccaata ctcgatcctc atgtccgtgt actcgaccgt cttgccggca
     1261 cggggagaat ggatgaactg gttgttcccg gcgtagatgc cgacatgcga aaccccggca
     1321 ccataggtat ggaaaaaaac aagatcgcct ggttgtgggg tctgaatttt ctggagctgt
     1381 ccgaattgac cggccgcggt atgggggagc cggacaccga cctgtccaaa aacatagagg
     1441 acaaaacctg aacaatcaaa cccgcgggga tcactgccgc cataccggta gggggtcccg
     1501 agatagcggc gtgcgattgt tacaacgtct ccccgtttgc ctgcgggctg tactgccccc
     1561 gtgggagtat tggtcccttg tgccctgctg accgttgtca tgcacccggt gagacacaag
     1621 agcaggagca tcggcagcat gccgtgtgat cgtatttgca tgggtcctcc gtcaggattg
     1681 tgcattccct gtcaatatcg gcggaatgaa taccggatct gacactgcaa gaacggttcc
     1741 gttcatgtgg ccagacactc atacaaaaaa cagcacgctg aattcctgcc gagaactcct
     1801 gtcaccgggg catccttcat ggtatactc
//
LOCUS       DAOSHE010000438         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_65242_length_1824_cov_2.839457, whole genome shotgun sequence.
ACCESSION   DAOSHE010000438 DAOSHE010000000
VERSION     DAOSHE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65242_length_1824_cov_2.839457"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PLD82_09815"
     CDS             complement(<1..1445)
                     /locus_tag="PLD82_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG65717.1"
                     /translation="MNRLQTISRWVQEDLWNDAILEGRSKAVRFGIIRLRMLVLEWRL
                     FFENRVMQTAGSLSFTTILSLIPLLAVLFMMFKLFDGRNVLQTGVKPHLYTLLAPGAG
                     EELTQAIDRMLQSASVETLGIVGVIFLFGSVYMMFFSVEEAFNKIWNVPKNRRFLDQL
                     RSYGVILMAVPVFTILSFMVTNHVRELGESIVGGFSLFFGETLFPFFMVVLAFYTLLR
                     VMPNCAIRGSRAWHGAVLGAVLYLVSKEVFVYYTSLAVSTNIIYGSLAALPIFFLWLF
                     IVWIIVLYAVIVVFVRHNLEHLTALERSKGINRTDELRIGLMTAMILARDALLPGGGE
                     PGSTVTGMASETGAPARDVRMIVERMEQARLLVRVAGKEERFMLRKMSSILVVGPRGE
                     AEQIVDTLYQQGTVHLVDLTPEGPSGPGELQRIDEASLIEVDQLLLKVNGLLKLLPKP
                     RTAAEEPGAIDDSFRSLPLQEMLEVIRGRLE"
     gene            1671..>1824
                     /locus_tag="PLD82_09820"
     CDS             1671..>1824
                     /locus_tag="PLD82_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG65718.1"
                     /translation="MGPRRTLFLVFLRIGGGMIGGGYSMLPLIQREVEQRWQDKLVFS
                     EALPLAQ"
BASE COUNT          419 a          558 c          500 g          347 t
ORIGIN      
        1 cactcgagtc ggccgcgaat gacctcgagc atctcctgaa gcggaaggct ccggaacgaa
       61 tcatcgatcg ctcccggctc ctcggcggca gtgcgcggtt tcgggagcag tttgagcagt
      121 ccgttcacct tcagcagcag ctggtcgacc tcaatgagcg acgcttcgtc gatccgctgc
      181 agttcccccg ggcccgacgg gccctcgggg gtcaggtcga cgagatggac cgtgccctgc
      241 tggtagaggg tgtcgacgat ctgctcggcc tcaccgcgcg ggccgaccac caggatactg
      301 ctcatcttcc ggagcatgaa gcgctcctcc ttgccggcaa cccggaccag aagacgggcc
      361 tgttccatcc gctcgacgat catccgtaca tccctggcag gagccccggt ctcgctggcc
      421 atccccgtta ccgtcgaccc aggctcgcct ccgcccggca acagggcatc ccgggccaga
      481 atcatggccg tcatcaaccc gatacgcaat tcatccgtcc ggttgatccc tttgctccgc
      541 tcgagggccg tcagatgttc gagattgtgc cggacaaaaa cgacaatcac ggcatacaaa
      601 acgatgatcc agacgataaa aagccagagg aagaaaatcg gcagggctgc cagcgaaccg
      661 tagatgatgt tggtcgacac ggccagactc gtgtagtaga caaaaacctc tttcgagacg
      721 aggtagagaa cggcgccaag gacagctcca tgccaggccc ttgaccccct gatggcacaa
      781 ttcggcatga cccggagcag ggtgtaaaaa gcgaggacca ccatgaaaaa cggaaacagc
      841 gtctcgccaa aaaacaggga gaatccgccg acgatgctct cccccaactc ccggacatgg
      901 ttcgtgacca tgaacgagag aatcgtgaac accggaacag ccatgaggat cacaccatag
      961 ctgcgcaact gatcaaggaa ccgccggttc ttcgggacat tccagatttt attgaacgct
     1021 tcttcgaccg aaaagaacat catatagacg cttccgaaca aaaagatcac gccgacaatc
     1081 ccgagggtct caacggaagc ggactggagc atccggtcaa tggcctgtgt cagttcttct
     1141 cccgccccgg gagcgagcag ggtataaaga tggggtttga caccggtctg gaggacattg
     1201 cgcccgtcaa agagcttgaa catcatgaag aggaccgcaa gcagcgggat cagggagaga
     1261 atcgtggtga acgaaagcga accggccgtt tgcatgaccc ggttttcaaa aaacagccgc
     1321 cactcgagga ccagcatccg gagccggatg atcccgaatc ggaccgcttt gctccggcct
     1381 tccaagatgg catcattcca gagatcctct tgcacccacc gggaaatcgt ctgcagtctg
     1441 ttcatcaatc accctccaca accgtccata cgggaataat agtacctggc cccgtacaga
     1501 tgcaaaccgg tctgccatcc tcacatgacg gttccatgac ccttcacatc ctgttttcaa
     1561 taggttcatg ccactggcat gcttgactga acaccggggt gactgctgtt tgaggattcg
     1621 ggtcagatgg cagccctgcc cgtcgcgacg ctttttgcta tacttttttt atgggacccc
     1681 gcaggacgct ctttttggtt ttcctccgca tcggcggcgg gatgatcggt ggcgggtatt
     1741 cgatgctccc cctcatccag cgggaggtcg aacagcgttg gcaggacaag ctggtttttt
     1801 ccgaagccct gcccctggcc cagg
//
LOCUS       DAOSHE010000439         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_65498_length_1819_cov_4.227324, whole genome shotgun sequence.
ACCESSION   DAOSHE010000439 DAOSHE010000000
VERSION     DAOSHE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65498_length_1819_cov_4.227324"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /gene="argS"
                     /locus_tag="PLD82_09825"
     CDS             complement(<1..1501)
                     /gene="argS"
                     /locus_tag="PLD82_09825"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG65719.1"
                     /translation="MIQEMTDRIANVLPGITGLEADAVCAAITRPPKPEMGDLAFPCF
                     PLAKVRGKAPQVIAAELVPEAAAALAGSGIIVESAGPYLNFRFDRKKVAAGIAARAMS
                     GTPLVDDCGRGRTLVIDFSSPNIAKPFSMGHLRSTSIGNALSRIYAALGWKVVRINHL
                     GDWGTQFGKLICAFRLWGERERLEKEDIAYLMELYIRFNDAAKADPALDDAARAAFKE
                     LEDGGAEAHELWQVFRDASLREFERIYGILDVSFDSFAGESFYNDKVDGAVQTITDAG
                     LASTSEGALVVEMDEGSKEPPCMIRKSDGATTYAARDLAALFYRQKEYAFDRLLYVVG
                     APQSLHFRQVFTVLRKLGLAWHERCQHIPFGQVLLEGEMMSTRRGNVIFLEDVIKEAE
                     SRALEKMLANAVSDDESGSEDSTASVDISQATDEQRARARILAVSSIIFFDLKNGRIK
                     DVDFSWDEILNPHGETGIYLQYAHARIHGIMRKFEDRTGLSIDEIRRSSP"
BASE COUNT          350 a          512 c          596 g          361 t
ORIGIN      
        1 cgggagagct gcgccggatc tcgtcgatcg agagtccggt gcggtcctcg aatttgcgca
       61 tgatcccgtg gatgcgggca tgggcgtact ggaggtagat cccggtttcg ccgtgcgggt
      121 tgaggatctc gtcccaggaa aagtcgacgt ccttgatgcg cccgtttttg aggtcgaaga
      181 aaataatgga agaaacagcg aggatgcggg cacgcgcacg ctgttcatcc gtggcctggc
      241 tgatgtcaac ggaggccgtg ctgtcctcgg acccggactc gtcgtcagag acggcattgg
      301 cgagcatttt ttcgagcgcc cgggactcgg cctccttgat cacgtcttcg aggaagatga
      361 cattgccccg gcgggtggac atcatctcgc cctcgagcag gacctggccg aaggggatgt
      421 gctggcaacg ctcgtgccag gcgagcccga gcttgcgcag gacggtaaag acctggcgaa
      481 agtgcaggga ctgtggcgcg ccgaccacgt agagcaggcg gtcgaaggcg tactcctttt
      541 ggcggtagaa gagggcggcc agatcgcggg ccgcataggt ggtggcccca tccgacttgc
      601 ggatcatgca gggcggttcc tttgagcctt cgtccatttc gacgacgagg gcgccttcgc
      661 tggtgctggc cagtcccgca tccgtgattg tctggaccgc gccatcgacc ttgtcgttgt
      721 aaaaactctc tccggcaaag ctgtcaaacg agacatcgag gatcccgtag atgcgctcga
      781 attcacgcag ggatgcgtct ctgaagacct gccagagttc gtgggcctcg gcaccaccat
      841 cctcgagttc cttgaaggcg gcccgggcgg catcgtcgag ggccggatcg gccttggccg
      901 catcattgaa gcggatgtag agctccatca ggtaggcgat gtcttccttt tcgagacgct
      961 cgcgctcacc ccagaggcga aacgcgcaaa tcagcttgcc aaactgggtt ccccagtcgc
     1021 cgaggtggtt gatccgcacc accttccaac ccagggctgc gtagatgcgg gagagggcgt
     1081 tcccgatcga ggtcgaacgc aggtgcccca tcgaaaacgg ttttgcaata ttgggtgacg
     1141 aaaagtcgat cacgagggtg cgcccccggc cgcaatcatc gacgagcggg gtgcccgaca
     1201 tggcccgggc tgcaatcccg gcggcaacct ttttgcggtc aaagcggaag ttgaggtagg
     1261 ggccggctga ctcgacgatg atcccgctgc ctgcgagtgc tgccgccgcc tcgggtacca
     1321 gctctgccgc gatcacctgc ggggccttgc cacgcacttt ggccagcggg aagcagggga
     1381 aggccaggtc acccatctcg ggctttggcg gccgggtgat ggctgcgcac acggcatccg
     1441 cttcaagacc ggttatcccg gggagtacgt tcgcaatgcg gtcagtcatt tcctggatca
     1501 tggtccacct cgttggtcat taatatgggg aacagcctcc ggcttgcggg aagcggcagg
     1561 tgcgaacaaa gatactcttt cggcgttttc ccggacaagg ttgggctggt ggcagcggta
     1621 tgtcattctt cgcgccgggg gtgatatcag gtatatttta cgggtagaac gtactgcggg
     1681 ggacatgcat ggataccatt gaaagcctgc aaaaagcgct tgcgtccaag gacgctgtca
     1741 tcctgatgct gctcacgcag aacgaagagt tgaaaaaggc cgttgaaaat ctggacaaac
     1801 tggtcgccat gtatgaccg
//
LOCUS       DAOSHE010000440         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_65553_length_1818_cov_3.768009, whole genome shotgun sequence.
ACCESSION   DAOSHE010000440 DAOSHE010000000
VERSION     DAOSHE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65553_length_1818_cov_3.768009"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /gene="dut"
                     /locus_tag="PLD82_09830"
     CDS             complement(<1..300)
                     /gene="dut"
                     /locus_tag="PLD82_09830"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG65720.1"
                     /translation="MDESVRFRLVTPGAAIPVRATPHAAGYDCVAAMETAVTLQPGER
                     YLVPLGFEMALPATMEAQIRPRSGLALKHGVTLANAPGTIDADYRGEVRVILVNLG"
     gene            complement(293..1561)
                     /locus_tag="PLD82_09835"
     CDS             complement(293..1561)
                     /locus_tag="PLD82_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG65721.1"
                     /translation="MRKYVFSNGITLVVERMPRVETVSFGLWQRVGSSNESPLTNGLT
                     HFLEHMLFKGTSEHSASDIAIRMDSVGAHMNAFTTREKTCFYANVVARHAPMVIGLLK
                     AMYYDSVFPPKEIQREKQVVIQEIHMYDDSPDDYIHDRFVEQLWPDHPLGFSISGKVE
                     NIEAITRARLLDFFNKHYRTDRLVISAAGMIDPDTLAREIETWPLRGSGDPALPTQPP
                     EPRFTLAAQKKKLEQVQLLIGMPGVHKRHPWRVAIALVNQLWGGSMSSRLFQKIREEH
                     GICYSIYTFPALMQETGYFGISCATSAEYLDTVLTLIRRENELLLKKGISSEEFLRSK
                     EQLKSSLLIGRESVESRMNYIAMQELVFGRGEPLSAVLKRIDAVTMEDIRDVISYILG
                     SERFAVRTLGPIDAMPILKKHFPAGETHRG"
     gene            complement(1574..>1818)
                     /locus_tag="PLD82_09840"
     CDS             complement(1574..>1818)
                     /locus_tag="PLD82_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG65722.1"
                     /translation="SDVEVGEIYNGIVKKIMDFGAFIEILPGKDGLCHISKLASKRVN
                     KVSDVVREGERIRVRVVEVDKLGRINLSAVNVDDPE"
BASE COUNT          404 a          522 c          513 g          379 t
ORIGIN      
        1 tcccaggttg acgagaatca cccgcacctc accacgataa tccgcatcaa tcgttccggg
       61 ggcgttggcc agcgtcaccc catgcttgag ggccagcccg ctccggggcc ggatctgtgc
      121 ttccatcgtt gcagggaggg ccatctcgaa gccgagcggg acgaggtagc gctcgcccgg
      181 ctgaagggtg acagcagtct ccatggcagc aacacagtca tacccggcgg catggggcgt
      241 cgcacggacc gggatggcag cacccggagt gacaagccgg aagcggaccg attcatccac
      301 gatgcgtctc cccggcggga aaatgctttt tgagaatcgg catggcatca atcggaccga
      361 gtgtccggac ggcaaagcgt tcgctcccca gaatatagga gatcacatcc cggatatcct
      421 ccatggtgac ggcatcgatc cgcttgagaa cggcgctcag tggttcgccc cttccaaaaa
      481 caagttcctg catggcgatg taattcattc tcgattcgac actctcccgg ccgatcagga
      541 gggaactctt caattgttcc ttggagcgga gaaattcttc gctggaaatc ccctttttga
      601 gaagaagctc gttttcgcga cggatcaggg tcagaacggt atccagatac tcggcggagg
      661 tcgcacagga aatcccgaag tacccggttt cctgcatcag tgcagggaag gtataaatcg
      721 aatagcagat tccgtgctct tcacggatct tctggaagag ccgtgagctc atgctcccgc
      781 cccagagctg gttgaccagg gcgattgcca ctcgccaggg atggcgtttg tggaccccgg
      841 gcatcccgat cagaagctgc acctgctcca gctttttctt ttgtgccgcc agggtaaagc
      901 gcggctcggg gggctgggtc ggcagggcgg gatccccgct cccccggagg ggccaggtct
      961 cgatctcgcg cgccagggta tcgggatcga tcatgcccgc cgcggagatc acaagccggt
     1021 cggtccggta gtgcttgttg aaaaaatcca gcaagcgagc ccgggtgatg gcctcgatat
     1081 tctcgacctt gcccgaaatg gagaatccga gagggtggtc gggccagagc tgttcgacaa
     1141 accggtcgtg gatataatca tcaggagaat cgtcgtacat atggatctcc tggatcacga
     1201 cctgcttttc tctctgaatc tcttttgggg gaaagacgga atcgtaatac atggccttga
     1261 ggagaccgat caccatcggc gcatgacggg caacaacgtt ggcgtaaaaa cacgtcttct
     1321 cacgagttgt gaaggcgttc atgtgcgcgc caacggaatc catccggatc gcgatatcac
     1381 tcgcggagtg ctcgctcgtt cccttgaaga gcatatgctc gagaaaatgc gtcagaccat
     1441 tggtcaaggg gctctcattc gaggacccca cacgttgcca gagaccaaag gagaccgtct
     1501 cgaccctcgg catccgttcg acaacaagag ttatgccgtt tgaaaagaca tactttctca
     1561 cgccagaagc cgattattcg ggatcatcga cgttgacagc gctcagattg atacggccaa
     1621 gcttgtcaac ctcgacaaca cgcacccgga tccgttcacc ctcgcggaca acatcgctga
     1681 ccttgttgac tctcttgctg gccagcttcg agatgtgaca gagtccatcc ttgcctggaa
     1741 gaatctcgat aaaggcgcca aaatccatga tctttttgac aattccattg tagatctcgc
     1801 ccacttcgac atcggagg
//
LOCUS       DAOSHE010000441         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_65555_length_1818_cov_3.698242, whole genome shotgun sequence.
ACCESSION   DAOSHE010000441 DAOSHE010000000
VERSION     DAOSHE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65555_length_1818_cov_3.698242"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLD82_09845"
     CDS             complement(<1..1101)
                     /locus_tag="PLD82_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOG65723.1"
                     /translation="MKTVFGVLFLFMLSTEVLPALEVRLELSSTTAAVGEPVYAQVHI
                     NDAAGVDCLIEFPPTPAFTRREAGVSQNHSVRIINSRVERSSTWIRNWVFIPSQAGRF
                     VIGPATCKIGGRVLTTQTVTLEVTTASAKRPPTRNRRGGLFDFFEHDQMGGQDEEETE
                     VRLETGFDRPSVWAGEQVVHYIDILSTRQSLINVEFAQIQRHGFWEVQSDPRQQPKPE
                     KITRQGRDWFRYRYISSYLFPLTSGQKSVEPFAVRVHYDRFWPKSVVLQSGKLELAVR
                     ELPRDPEGLFSGAVGSFTLEEIFEPVSGYACDQPFVMTLILSGEGNVRAAGEPQIAQT
                     TNFRFMQPQIETEVRAHQGRMHGVRRWKYLVYP"
     gene            complement(1098..1754)
                     /locus_tag="PLD82_09850"
     CDS             complement(1098..1754)
                     /locus_tag="PLD82_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG65724.1"
                     /translation="MTKTVLLTAVLMMLTVPLVSTTDAQAVLDRAQKILESDPDNLEA
                     RYNHGWASATLGKHAESERDFKAVVAKADGKMKTDSLFNLGYVLFMQKNLQGALEAWR
                     AGLRHAPDDRDMQYNYTVVRRLLKQEEKKKDEKKDDDTDKKSGDKKNSDSGAQQPGDQ
                     KNDEKQSSQKNPGGQQPRTGEMTREEAVRLLKALQEQEQKANPQDKQIMGGVRRGKDW
                     "
BASE COUNT          383 a          533 c          431 g          471 t
ORIGIN      
        1 cgggtagaca agatatttcc agcggcggac accatgcatc cgtccttgat gtgctcgaac
       61 ctcggtttcg atctggggct gcatgaaacg gaaattcgtt gtctgtgcaa tctgtggctc
      121 gcccgcagcc cggacgtttc cttccccgct caatatcagt gtcatgacaa aaggctggtc
      181 acaagcatac ccgctcactg gctcaaatat ttcttccagt gtaaaggatc caacggctcc
      241 cgaaaacagg ccttccggat cacgcggcag ctccctgacc gcaagttcca gctttcccga
      301 ttggagaaca acggattttg gccaaaatct gtcataatgg acccgaacgg caaaaggttc
      361 aacggatttc tgtccggatg tcaaaggaaa gagataactg ctgatatacc gatatcgaaa
      421 ccaatcccga ccctgcctcg tgattttttc cggttttggc tgttggcgag gatcggactg
      481 gacttcccaa aaaccatgtc gttggatctg ggcgaactcc acattgatca gggactgcct
      541 ggtcgagagt atgtcgatat agtgcacaac ctgctcgcct gcccacaccg atggacggtc
      601 aaaacccgtc tcgagccgga cctcggtttc ctcttcatcc tggccaccca tctgatcatg
      661 ttcaaaaaaa tcaaacagtc cgccccgtct gtttcttgtc ggaggccgtt tggcagacgc
      721 tgtagtcacc tcgagagtaa ctgtctgtgt tgtcaggacc ctcccgccta ttttgcaggt
      781 cgccggacca atgacgaacc gcccagcctg actgggtata aacacccagt tcctgatcca
      841 ggttgaactc ctctcaaccc gggaattgat gatacgcacc gaatggtttt gcgagactcc
      901 ggcctcccgt cgggtgaatg ccggagtcgg tgggaactcg atcaggcaat ccactccagc
      961 cgcgtcattg atatgcacct gtgcatagac cggttctcca acggcagccg ttgtcgaaga
     1021 gagctcgagt ctgacctcga gtgccggaag aacttctgtc gagagcataa acagaaacaa
     1081 taccccgaat acggtcttca ccagtctttt ccccggcgga ctccgcccat gatttgttta
     1141 tcctgcggat ttgccttttg ttcctgttcc tgcaaagcct tgaggagccg gactgcctcc
     1201 tccctggtca tctccccggt tcgcggttgc tgtcctccgg gatttttttg actggattgt
     1261 ttttcgtcgt tcttttggtc tccaggctgt tgagcaccgg aatcactgtt ttttttgtcc
     1321 ccggatttct tgtctgtatc gtcgtctttc ttctcgtcct tcttcttctc ttcctgcttg
     1381 agcaatctcc tgacgacggt atagttatac tgcatgtccc tgtcatccgg agcatgtcgc
     1441 agaccggctc gccaagcctc gagagcaccc tgaagatttt tttgcatgaa caggacatat
     1501 cccaggttga agagactgtc ggtcttcatc tttccgtctg cctttgcaac aacggccttg
     1561 aaatcccgtt cagattcagc gtgctttccc aaggtcgctg aagcccatcc atgattgtat
     1621 cgtgcttcca gattgtccgg atccgactcc agtatctttt gcgcccggtc aagaaccgct
     1681 tgggcatcag tggtcgagac cagaggaacc gtcagcatca tcaacaccgc cgtcaacagt
     1741 accgttttgg tcatacccgc ttcctttcgg ggattcccaa tcccaatgtc aaaagaacca
     1801 gtcccgcacc gagaaaca
//
LOCUS       DAOSHE010000442         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_65608_length_1817_cov_3.292849, whole genome shotgun sequence.
ACCESSION   DAOSHE010000442 DAOSHE010000000
VERSION     DAOSHE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65608_length_1817_cov_3.292849"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLD82_09855"
     CDS             complement(<1..949)
                     /locus_tag="PLD82_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG65725.1"
                     /translation="MISAEIQRAIEAWQTPPFDEGTRREITNLVDAGNEQELHDRFHT
                     RLAFGTGGLRGIMGAGTNRMNRHTLAAATRGLADYLKESVSSAQDAGVVIGYDTRQNS
                     RFFAETTAAILAGDGFRTFLFPTLCPVPMLAASIRLLGCAAGVMITASHNPPKYNGYK
                     VFWSDSSQVVPPHDTGIITRVNAIEPGSITWPDFGQLLSSGKIMDVPQSVIDEYYSLV
                     LACRQYPGQITEACQQLRIVYSPLHGAGNLPVRHILSRAGFSVVSVVASQEQPDGTFP
                     TVTRPNPEEEAAMRLAVLQAVEENADLVLATDPDSDRVGV"
     gene            1199..>1817
                     /locus_tag="PLD82_09860"
     CDS             1199..>1817
                     /locus_tag="PLD82_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOG65726.1"
                     /translation="MRIPEIVILGRPNTGKSTLFNRLSGRRDAVIHPKAGVTRDRKAT
                     RILVREGLEALLIDTGGLFPDDPDDFSGDIERQAQLAIQTADLVIFLVDATAISGLDH
                     DFAEMVRRISKPVLVVANKMDRTRDGVLPPEVYELGFEHIIGISANHGTGNDELKLKM
                     AGILEEAVKLEAAQGLKEDLSFVLIGRPNVGNSSLGHSVLPHMPAH"
BASE COUNT          361 a          559 c          523 g          374 t
ORIGIN      
        1 ccacgccaac ccggtcggaa tccggatcgg ttgccaggac cagatcggca ttctcttcaa
       61 cggcttggag gacggcgagc cgcatggccg cttcctcttc gggattgggg cgggtgaccg
      121 tcgggaaggt tccgtccggc tgttcctgac tggcaacaac ggagaccacg ctgaacccgg
      181 cccgggagag gatatgccgg acgggaagat tgccggcacc atgcaacggg gaatagacaa
      241 tcctgagctg ttggcaagcc tccgtgatct gtccgggata ctggcgacag gcaaggacca
      301 ggctgtaata ctcgtcgatg accgattgtg gcacatccat gatcttcccg ctggacaaga
      361 gctgcccgaa atccggccag gtgatgctcc ccggctcgat ggcgttgacc cgagtaatga
      421 tgccggtatc gtgtgggggg acaacctggc tcgagtcaga ccagaagact ttatatccgt
      481 tgtactttgg gggattgtgg ctggccgtga tcatcacgcc ggctgcacag cccagaagac
      541 ggatcgaggc ggcgagcatc gggaccggac agagcgtcgg aaagagaaag gtgcggaatc
      601 catcgccggc cagaatcgcc gcggttgtct cggcaaagaa ccgggagttc tgtctcgtat
      661 cgtacccgat cacgacaccg gcatcctgtg cggatgaaac gctctccttg aggtagtcag
      721 ccagtccccg ggttgccgcc gccagtgtat gccggttcat ccggtttgtt ccggcaccca
      781 tgatccccct cagtcctccc gtgccgaagg ccagccgggt atggaaccgg tcgtggagtt
      841 cctgttcgtt tccggcatcc acaaggttgg tgatttcacg cctcgtcccc tcgtcaaaag
      901 ggggggtctg ccatgcttcg attgctcgct gtatctccgc agaaatcatg tttttttgct
      961 ccatgtcgtg tttgatgccg gattgcccgt tcgctgctct gatagtacac cgaatccggg
     1021 gtgtggagcc acatctttgc ggaccctcga gtctcctgga gtaccccgca ttcacccttc
     1081 tgttcatacc acagatgaaa aacctggcca cgtgggaatt gacccgatca cgcccatgcc
     1141 catcccgctt ccccggacgg attgactcag ggcaaggctc ctgagtacca ttaaaggcat
     1201 gcgcattccc gaaatcgtca ttcttggccg ccccaacacg ggcaaatcca ccctgttcaa
     1261 ccggctctcc ggtcgcaggg atgctgtcat ccatccgaaa gcgggtgtga cccgcgaccg
     1321 caaagcgacc cggatcctgg tccgtgaagg gcttgaagcc ctccttatag acacgggggg
     1381 gctctttccc gacgatcctg acgatttctc cggcgacatc gagcgacagg cccaattggc
     1441 tatccagacg gcggacctgg tcatctttct cgtcgatgca accgccatca gcggcctgga
     1501 ccacgatttt gccgaaatgg tccgccggat cagcaaaccg gtcctcgtgg ttgccaacaa
     1561 gatggaccgg acccgtgacg gcgttcttcc acccgaagtc tacgaactcg gattcgagca
     1621 catcatcgga atctctgcca accatgggac cggcaatgat gagctgaaac tcaagatggc
     1681 cgggattctg gaggaagccg tcaaactcga agcggcacag ggtctcaagg aggatctctc
     1741 cttcgtcctg atcggacgcc ccaacgtcgg caattcctct cttggccact ctgtgctccc
     1801 tcatatgccg gcccacg
//
LOCUS       DAOSHE010000443         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_66355_length_1803_cov_3.299771, whole genome shotgun sequence.
ACCESSION   DAOSHE010000443 DAOSHE010000000
VERSION     DAOSHE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66355_length_1803_cov_3.299771"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /gene="coaE"
                     /locus_tag="PLD82_09865"
     CDS             complement(<1..566)
                     /gene="coaE"
                     /locus_tag="PLD82_09865"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG65727.1"
                     /translation="MGGSPGGRPVLGLTGMMASGKNMAAEILRCEWGCAVIDLDRLGH
                     HVLTRLTGPLLAAFGGGILRSDDAGGGIDRAALGRIVFSDPGALSRLESIVHPEMVRE
                     VREILDEDRSPACVLNAAILHRMGLDTLCHHVVYISVPANSLVERIMERNGLSREAAL
                     ERLAGQEDVNRSGKSADTVLENDASPGI"
     gene            complement(566..>1803)
                     /locus_tag="PLD82_09870"
     CDS             complement(566..>1803)
                     /locus_tag="PLD82_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOG65728.1"
                     /translation="LVSNELESVQNTIELPLAPVLAGMELAGIRVDHTRLAAGSERLA
                     KRLAELESAIYAGAGMEFNLNSPKQMGSVLFEKLALPPVKKTKTGYSTDEEVLHELAR
                     QHEVPKLILEYRRAYKLKHTYLDVLPGLANPVTGRIHTSFNQAVTATGRLSSSDPNLQ
                     NIPVREDAGREIRRAFVPEPGCLFAGADYSQIELRILAALSGDPALVAAYQAGEDIHR
                     RTAARIFGIDEDAVDRERRGIAKAINFGVIYGQTAYGLSRDIGISRTDADSFIRTWFE
                     IYSGVREYAESVIERASQEGQVRTWFGRIRKIPELASRNRSVRQMGERLALNTLVQGT
                     AADLIKIAMVGVYTRLAQECPAARLLLQVHDELLVEAPAGDSGLVAQILEEEMRRPWP
                     FAVPLEIGSGQGASWDEVH"
BASE COUNT          362 a          585 c          502 g          354 t
ORIGIN      
        1 aagatcccgg gcgaggcatc gttctccaag actgtgtcgg cagattttcc ggaacggttt
       61 acatcttcct gtccggcaag ccgttccagc gccgcctcgc gggagagccc gtttcgctcc
      121 atgatgcgct cgacaaggct gttcgcaggc acggagatat acactacgtg atgacagagg
      181 gtatcaagtc ccatccggtg gaggatcgcc gcattcaaga cgcaggccgg gctccggtct
      241 tcatcaagga tttcacggac ctcgcgcacc atctccggat ggacaatgga ctcgagtcgg
      301 ctcagcgcac ccggatcgga aaaaacaatc cgcccgagcg ccgcccggtc gatcccgccc
      361 ccggcatcgt ccgatctgag gattccgccg ccgaaagccg ccagcagggg tcccgtcaac
      421 ctggtgagga catgatgccc cagtcggtcg agatcgatga ccgcacagcc ccactcgcac
      481 ctgaggattt cggccgccat attcttgccg ctggccatca tacctgtcaa gccgaggacc
      541 ggccggccac cgggtgatcc acccatcagt ggacctcatc ccagcttgcg ccttgcccgg
      601 acccgatctc gagaggaacc gcaaagggcc agggacgccg catctcctcc tcgagaatct
      661 gcgcgacaag accggaatcc ccggccggag cctccaccag caactcgtca tgtacctgca
      721 gaagaagcct ggccgcggga cattcctgtg ccagacgcgt atacaccccc accatcgcga
      781 ttttgatcag atcagcggcc gtcccctgga cgagggtatt cagggccaac cgctcaccca
      841 tctggcggac agacctgttc cggctggcca gttcggggat cttcctgatc cttccgaacc
      901 aggtcctgac ctgcccctcc tggctcgctc gttcgatcac ggattcggca tattcccgga
      961 caccggaata gatttcgaac caggtccgga taaagctgtc cgcatctgtc cgtgatatcc
     1021 cgatatcccg ggagagacca taggccgtct gtccgtagat caccccgaaa ttgatcgcct
     1081 tggcaatgcc ccttcgctca cggtcaaccg cgtcctcgtc aatcccgaaa atccgggctg
     1141 ctgtcctcct gtggatgtcc tcaccggcct ggtaggcggc caccagtgcc gggtcaccgg
     1201 aaagggccgc gagaatccgc aattcgattt gtgaatagtc cgcgccggcg aagagacaac
     1261 caggttcggg aacaaaggct ctccggatct cccgaccggc atcctcgcgg acgggaatat
     1321 tctgcaggtt gggatcactc gaggagagcc gtcccgttgc cgtcacggcc tggttgaaac
     1381 tggtatggat ccggcctgtg acgggattgg ccagcccggg cagaacatcc agataggtat
     1441 gcttgagctt gtaggcgcgt cggtattcga ggatcagctt tggcacctcg tgctgtcgtg
     1501 caagctcatg aagaacctcc tcgtccgtgg aatacccggt ctttgttttt ttgaccggag
     1561 gaagcgcgag tttctcgaag agcacactgc ccatctgttt tggcgaattg aggttgaact
     1621 ccatcccagc gcccgcataa atcgcggatt caagttcagc aagccgcttt gccagcctct
     1681 cgcttcccgc tgcgagacgt gtatggtcaa cccggatccc ggccaactcc atcccggcca
     1741 aaacaggtgc cagaggaagc tctatcgtat tctggacgga ctcgagttcg ttggatacca
     1801 gaa
//
LOCUS       DAOSHE010000444         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_66622_length_1798_cov_2.786575, whole genome shotgun sequence.
ACCESSION   DAOSHE010000444 DAOSHE010000000
VERSION     DAOSHE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66622_length_1798_cov_2.786575"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLD82_09875"
     CDS             complement(<1..186)
                     /locus_tag="PLD82_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65729.1"
                     /translation="MKTLQIILVLLLCSAPLAATTVFSERYAGSNVVKVYVTEYESQA
                     DLKVFKVQYSSQASRNDG"
     gene            272..892
                     /gene="yqeK"
                     /locus_tag="PLD82_09880"
     CDS             272..892
                     /gene="yqeK"
                     /locus_tag="PLD82_09880"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOG65730.1"
                     /translation="MTRHEADAIARSHLEGFRLEHSLSVATLAGEMGRAFGLDDDALS
                     VAGVLHDIAKPMDPDRQRVLALAWHDAQPESVWSETPALWHAPAAAWLLVHEYGLDDQ
                     RIVQAIVRHTTGCNGMTRFDECLYAADFLDPVRVFDGQSVVWTLIRTDFDAGLLEMCR
                     QTILSVVERGRTLDQASVAYYNEIAGRLADPGHLPRGFTYQGAAHV"
     gene            885..1400
                     /locus_tag="PLD82_09885"
     CDS             885..1400
                     /locus_tag="PLD82_09885"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG65731.1"
                     /translation="MSDVFVIDKPNFYALAVHKVVGTFSLGKAMKPAYCMVMEKMQET
                     GLICPRGMVPFTRYNRIDWSEMEKRGLRATLKMLFAKKWDMDIGIPVDRMTEIRGEVS
                     FLDCRLGRCVSTVHIGPYQKVSQAYTRVLKMARDQGLALADHSYELYLNDPNDVPPGE
                     LRTEILIPIIL"
     gene            1504..>1798
                     /locus_tag="PLD82_09890"
     CDS             1504..>1798
                     /locus_tag="PLD82_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65732.1"
                     /translation="MNGRLALSGGVIVRFWLILIPALCLWTACHKPDERIIPVGTPEE
                     RAWLATNTHLLVYAPDPAYPPFEFCDEHDGKTRGMARDFLEALEKRLGVRFMKK"
BASE COUNT          389 a          438 c          517 g          454 t
ORIGIN      
        1 accgtcattc ctggaggcct gactggaata ctggactttg aaaaccttga ggtctgcttg
       61 actttcgtat tcggtgacgt acacttttac aacattggac cctgcgtacc gttcggaaaa
      121 cacggttgtg gcggcaagcg gggcactgca caagagcagg accaaaatga tctggagagt
      181 tttcatagat gctccttttt tccggatgtt caataaaata tactgactgt tttgctccag
      241 gaacaggtcg gattgttcgg ggacatgccc gatgacccgt cacgaagcgg atgcgatcgc
      301 gcggagccat ctggaagggt tccggctcga acactccctc tcggtagcaa cattggcagg
      361 cgagatgggg cgggctttcg gtctggacga tgacgccctc tccgtcgcgg gcgtcctgca
      421 cgatatcgca aaaccgatgg atcccgatcg acagcgtgtt cttgctctgg cctggcatga
      481 cgcgcaacct gaatcagtct ggagcgagac accggcgctc tggcatgccc cggcggctgc
      541 ctggctgctc gtgcacgagt atggtctcga tgatcagcgg attgtccagg ctattgttcg
      601 tcatacaacc gggtgcaatg gcatgacacg ttttgacgag tgtctctatg cggccgattt
      661 cctcgatccg gttcgtgtct ttgacggaca gtctgttgtc tggaccctga tccggactga
      721 ttttgatgcc gggttgctcg agatgtgccg acaaacgatt ttgagtgtgg ttgaacgcgg
      781 tcgcactctg gatcaggctt ccgtagccta ttacaatgag attgcaggaa ggcttgcaga
      841 tcccggacat ctcccccggg gattcacata ccaaggagcg gcacatgtct gacgtttttg
      901 tcattgataa accaaatttt tatgctttgg cggtccacaa ggtggtggga acattttcgt
      961 tgggcaaagc aatgaaaccg gcctattgca tggttatgga aaaaatgcag gaaaccggtc
     1021 tgatctgtcc ccggggaatg gttccgttta cccggtacaa ccgcattgac tggagtgaaa
     1081 tggaaaagcg cgggttgcgg gcaacgctca agatgctgtt tgcgaaaaaa tgggatatgg
     1141 atatcgggat tccggtggac aggatgacgg aaatccgggg tgaggtttcg tttctcgatt
     1201 gccggctcgg gaggtgcgtc tcgactgtcc acatcgggcc ataccagaag gtgtcccagg
     1261 cctatacccg tgtgctcaag atggcaagag atcagggact ggctcttgcg gatcactcgt
     1321 acgaattgta tctgaatgat ccgaacgatg ttccgcccgg agaattgcgg accgagattc
     1381 tcattccgat cattctctga gcaatgattt ctcgggtctg gctcagcctg cttcagtaca
     1441 gtaattctgg atgcaggagt tttatttttg ttccttgcgc aatcttgtga tacaatcagg
     1501 aatatgaacg gtcgtctcgc tctgtcgggg ggagtcattg tgcgattctg gttgatactc
     1561 atacccgctt tgtgtttgtg gacagcctgt cacaagccag atgaacggat cattccggtc
     1621 ggcacacctg aagagcgggc ctggttggcg acaaatacac acctcctggt ctatgctccg
     1681 gatcccgcct atccgccatt cgaattttgc gacgagcatg atggaaaaac gcgtggaatg
     1741 gcccgcgatt ttttggaggc tctggagaaa cgactcggtg tgcgattcat gaaaaaac
//
LOCUS       DAOSHE010000445         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_66724_length_1796_cov_2.630098, whole genome shotgun sequence.
ACCESSION   DAOSHE010000445 DAOSHE010000000
VERSION     DAOSHE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66724_length_1796_cov_2.630098"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLD82_09895"
     CDS             <1..627
                     /locus_tag="PLD82_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65733.1"
                     /translation="NKAEFEALSQIDPDAWKALVRAHLQDRLETAVKNSQGWGYGHKF
                     KETVFGTMRQQAILREALTNPITGDTSAYRRFSDFMDLLDVTNRIVYRNSRTTPLAVT
                     KEMIEREAGGLRGRLIEIAGSKLNWTKWADINRRLSAPEYLDRIYDAMMDPSTGQTLG
                     RIRQLSNPQKKAIETVAVVSAILGENVTDYQIDMSLDLYPERAESAKR"
     gene            953..>1796
                     /locus_tag="PLD82_09900"
     CDS             953..>1796
                     /locus_tag="PLD82_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65734.1"
                     /translation="MSKRWIAGLVVVAALLVIVACGYLAASDVSQLQGVTGKIGSEDI
                     YWGTGADDDTFDVDVSTYSGGTVTLTKVPSVSDNMSSIVKGRWYVSAAASIEDHSDVE
                     VPGSLAWVVAQIGSGNSGHIVIPPGNYVMADDFSLPAGIVLELASGALLDIDSGKTLT
                     IPSPRSLLASSTQQIFDGDGLVTFTYPGTVYPDWWQENTTPGTTDMVDAINAAMVAGG
                     AGATVQFNTATYLLSTINECADLPACAASGSPSYIENYFVNLLDDRTVQGTGWGTILK
                     IPDNL"
BASE COUNT          437 a          462 c          527 g          370 t
ORIGIN      
        1 aacaaggctg aattcgaagc cttgagccag atcgaccctg atgcctggaa agccttggtg
       61 cgtgcccact tgcaggatcg gctcgaaacg gcagtcaaaa actcgcaggg ctggggatat
      121 ggtcacaagt tcaaagagac cgttttcggt acaatgcggc agcaggccat attgagggaa
      181 gctctcacca atccgataac aggcgacact tcggcctata ggcgattttc cgatttcatg
      241 gacttgcttg atgtgacaaa ccgtattgtc taccgcaact caaggactac cccactggct
      301 gtaacgaaag aaatgattga aagggaggcg ggcgggcttc gtggcagatt gatcgagatt
      361 gccggcagca agctgaattg gacgaagtgg gccgacatca accgcaggtt gagcgccccc
      421 gaatatctgg acaggatcta tgatgccatg atggacccgt caaccgggca gaccctcggg
      481 aggatcaggc agctttccaa tcctcagaaa aaagcaatcg agaccgttgc cgtggtgagt
      541 gcaatcctgg gggagaatgt caccgattac cagatagata tgtcgctgga tctttacccc
      601 gaacgcgcag aatcagccaa gagatagggc ttacaaccag cgcgaaggca gccccgcatg
      661 tgacgcaaag aatgaggtcg atgaaaatgt ttccggtgct tttgatactt cccgagaaga
      721 cgtgaacagc gatgatggcg gagcaataca gtgtggcatg cttgaagatc aggaacataa
      781 cccctcctgt taggtgtgag gactcaataa tgatgaagta aaaacaagcc actgtcaagt
      841 gaaacacatt ttctcccggc ctgatcagcc gagggggagg gcaaccagaa gacccgccaa
      901 ccgtgcacgg cggaaggcgg gctttttatt ttgcagacaa ggagaacgag caatgtcaaa
      961 gagatggatt gccggtttgg tggtggttgc ggcgctgctg gtgattgtgg cctgcggata
     1021 cctggctgcc tctgacgtgt cgcagctcca gggcgttacc ggcaaaatcg ggagcgagga
     1081 catctattgg ggaaccgggg ccgatgacga cacgtttgac gttgacgtgt caacctattc
     1141 agggggtacg gtcacgctca ccaaggttcc cagcgtatcc gacaacatgt caagcatcgt
     1201 caaggggcgc tggtatgtat cggccgctgc aagcatcgag gatcattcgg atgtagaggt
     1261 tcctggatcc ctggcttggg tggtggctca gatcggtagc ggaaactcgg ggcatatcgt
     1321 gattccgccc ggcaactatg tgatggcgga tgacttttct ctacctgctg ggatcgttct
     1381 ggaactggca agtggggcac tgttggatat tgattccggc aaaacgctca cgatcccatc
     1441 gccaaggagt ctactggcct cctccactca gcagatcttt gatggggacg ggttggtcac
     1501 gttcacatat ccaggcacgg tctatcccga ctggtggcag gaaaacacca cacccggcac
     1561 aaccgatatg gtggacgcca tcaacgccgc catggttgcc gggggcgccg gggcaaccgt
     1621 gcaattcaat accgcgacct acctgctgag caccattaac gagtgcgcag acttgccggc
     1681 ctgcgccgct tcggggtcgc cttcctacat agaaaactat tttgtcaacc tgctcgatga
     1741 ccgtaccgtt cagggcaccg gctgggggac catcctgaag atacctgaca acctgt
//
LOCUS       DAOSHE010000446         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_67347_length_1785_cov_3.424277, whole genome shotgun sequence.
ACCESSION   DAOSHE010000446 DAOSHE010000000
VERSION     DAOSHE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67347_length_1785_cov_3.424277"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLD82_09905"
     CDS             complement(<1..287)
                     /locus_tag="PLD82_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65735.1"
                     /translation="MGVIPGPRAILNVIHSLLRMRTLIFQLARRDFKTRYLGSYLGVI
                     WGYIQPFISMLLLWFIFEIGFKSMPVQNFPFSIWLFTGMVPWLFFAEALQAA"
     gene            438..>1785
                     /locus_tag="PLD82_09910"
     CDS             438..>1785
                     /locus_tag="PLD82_09910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG65736.1"
                     /translation="MRIRSLINRLLPPGNTLRRMLVHWVRMVSGRRVYQLERLSGRAW
                     RRWLIRRGEPAILPLALQAVAGSLSAVIDCRRLQDGTDLCACLMGSGQSAVFLALDDL
                     VVDEVCLTRMGEMVHVGKVLVYGDDDRLDGKGCRLRPRLRPHYSRILFENIWYLGELV
                     ACQRERLLAFCEASGPDLAALSPREILSRFVLGLADREIGHCAQIVAHLGPGASTRDW
                     YRHQSVPVPALPPSLDVSVIIPNRDQAGMLERCLDSLQKYSGGHRLEIVIVENGSREA
                     ATFALYERLRRDPSVRILEWKAPFNYAAINNMAAAASRSGLLLFLNNDVEARQAWLQP
                     LAQYALRPEVGAVGAKLLYPSGRIQHAGMVLGMVGSAGHIFRFSPGHSDGYLGLLRFP
                     RDAGALTGACIMMRRDVFTRVGGFDEELAVTCNDVDLCLRVHAAGLRVVWTPGTELV"
BASE COUNT          309 a          511 c          572 g          393 t
ORIGIN      
        1 gctgcctgga gagcctcggc gaaaaagagc caggggacca tcccggtgaa gagccagatc
       61 gaaaagggga agttttgcac gggcatggac ttgaaaccga tctcgaaaat gaaccagagg
      121 aggagcatgc tgataaaggg ctggatatag ccccagatca ccccgagata ggatccaaga
      181 tagcgggtct tgaagtcccg tcgcgcaagc tgaaagatca gcgtccgcat ccggaggagg
      241 ctgtggatga cattgaggat ggcgcgcggc cctggtatca cgcccatggc ggggactcct
      301 tggatttttt gtgacttgac agataaaatt agcccgcccg cccggccgcg gccacagcct
      361 tgaaaaggga aaaacctgct acgccatttc ttgcaaaccg gcaggagtct ttgcccattg
      421 gggcatatat ttacggaatg agaatacgca gcttgatcaa cagactcctg ccgccgggaa
      481 acacgctgcg ccgcatgctt gtccattggg tgcgcatggt gtccggccgg cgcgtctatc
      541 agctcgagcg gctctccggc cgcgcctggc ggcgctggct gatccggcgt ggcgagccgg
      601 ccattctccc gctcgcactg caggctgtgg ccggctccct gtccgcagtg atcgactgcc
      661 gccggctgca ggatgggacg gacttgtgcg cttgtctcat ggggtctggt cagtccgcag
      721 tttttctcgc cctggatgat cttgttgtgg atgaggtttg tcttacccgg atgggagaga
      781 tggtccatgt cggaaaggtc ctggtctatg gtgacgatga ccgactcgac ggaaaaggct
      841 gtcgcttgcg tccccggctc aggccgcact atagccggat cctgtttgaa aacatctggt
      901 atctgggcga acttgtggca tgtcagcgtg aacgccttct ggcgttctgt gaggcgtcag
      961 ggccggatct ggcggcgctt tcaccccggg agatcctgag ccgttttgtg ctcggtctcg
     1021 cggatcggga gatcgggcat tgtgcacaga ttgtggccca cctcggaccc ggtgcttcca
     1081 cgagggactg gtaccgtcac cagtctgtac cagtcccggc gcttcctccg tcgctcgacg
     1141 tttcggtcat catccccaac cgggatcagg ccgggatgct tgagcgttgt ctggattcac
     1201 ttcaaaaata ttcgggtggc cacaggcttg agattgtgat tgtcgaaaac gggagccggg
     1261 aggcggcgac ctttgccctc tacgagcgtc tgcggcgtga cccgtccgtc cgtatcctcg
     1321 agtggaaagc gcctttcaac tatgctgcga tcaacaatat ggccgcagcc gcgagtcgct
     1381 ccggcctgct tctgttcctc aataatgatg ttgaagcccg ccaggcctgg cttcaacccc
     1441 tcgcgcagta cgcgctccgt cccgaggttg gcgctgtcgg ggccaagctc ctctatccgt
     1501 caggccggat ccagcatgcg ggcatggttt tgggcatggt cgggagtgcc ggccacatct
     1561 tccggttttc gccggggcat tcggatggct atcttgggct cttgcgcttt ccccgcgacg
     1621 cgggggcctt gaccggtgcc tgtatcatga tgcgccggga tgtctttacc cgtgttggcg
     1681 gttttgatga agagctggcc gtgacctgca acgatgtcga tctctgcctg cgggtccatg
     1741 cggccgggct gcgtgtggtc tggacgcccg ggacggagct ggtcc
//
LOCUS       DAOSHE010000447         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_67632_length_1780_cov_4.051594, whole genome shotgun sequence.
ACCESSION   DAOSHE010000447 DAOSHE010000000
VERSION     DAOSHE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67632_length_1780_cov_4.051594"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLD82_09915"
     CDS             complement(<1..1454)
                     /locus_tag="PLD82_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG65737.1"
                     /translation="MDRYDIIVIGGGHAGIEAAAAAVRIGRPGACQWPESEDVTTSGC
                     PLRVAIITPDPAAMGRMSCNPAIGGTAKGHLVREIDALGGLMGRITDQAAIQMRTLNL
                     SKGPAIHSTRAQADRERYEAVASAMLRQISDLVIIEGRVAAVSGEPVIGEQPQADPVS
                     TRPANDTADSLPGHSSGARFRVCGVRLSDGRTLGARCVILATGTFLGGILHTGLEASP
                     GGRVGEAPSQGLTDSLVALGLTSGRLKTGTPPRILRDSIDFSACEAQGGDPGVRPFSR
                     RTPLADFPYLTQVPCHVTWTHAETHDELRKGFDRSPLYTGRITGIGPKYCPSIEDKIV
                     RFAEKTRHQLFLEPEGLETPLYYVNGFPTSLPADVQEAALHTIPGLERAVIARPGYAV
                     EYDFFPASQIRHTLETKAVQGLYLAGQVNGTSGYEEAAAQGLMAGINAALACLGRVPC
                     ILGRHEAYIGVLIDDLISKVPLEPYRMFTSRAEF"
     gene            complement(1458..>1780)
                     /locus_tag="PLD82_09920"
     CDS             complement(1458..>1780)
                     /locus_tag="PLD82_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOG65738.1"
                     /translation="GELSVSVCTVAELVHGCHKSSDPRRNYLALLQFLLPYRILDWKN
                     RDVESYALIRAGLEKMGTPIGTIDTFIAAQARSRGLTLITNNVREFARVPGITIEDWS
                     QAEE"
BASE COUNT          347 a          569 c          534 g          330 t
ORIGIN      
        1 ctgaactcgg cccgggatgt aaacatgcgg tacggttcga gcggcacctt gctgatcagg
       61 tcatcgatca agaccccgat ataggcttca tgccttccaa gaatgcaagg taccctaccg
      121 agacaggcca gggccgcatt gatcccggcc atcaacccct gggcggccgc ttcctcgtag
      181 cccgatgttc cgttgacctg gccggcaaga tacaggccct ggacggcctt tgtctccagg
      241 gtgtgcctga tctggctggc gggaaaaaaa tcatattcga ctgcataccc gggacgggca
      301 ataaccgccc gttcaagtcc ggggatggta tgcagtgccg cctcctgcac atccgccggc
      361 agggatgtgg ggaatccgtt aacataatac agtggagtct ccagcccctc cggttcgaga
      421 aagagctgat gccgggtctt ttcggcaaag cggacgatct tgtcttcgat ggaaggacaa
      481 tacttgggcc caatcccggt gattctccct gtatacagcg gcgagcggtc aaaccccttg
      541 cgcaactcgt catgcgtctc ggcatgcgtc caggtgacat ggcaggggac ctgtgtcaga
      601 tacggaaaat ccgccagcgg agtacgccgc gaaaatggtc gcacacccgg gtcgccaccc
      661 tgggcttcgc aggccgaaaa atcaatggag tcccgcaata tccggggcgg tgtacccgtc
      721 ttgaggcggc cgctggtcag gcccagggcc acaagggagt ccgtcagccc ctggctgggg
      781 gcctccccca cgcggccgcc cggacttgcc tccagcccgg tatggaggat accacccaaa
      841 aaggtcccgg tcgcaagaat gacgcagcgc gcccccaatg tccgcccgtc gctcagcctg
      901 acgccacata cgcggaaacg tgcccccgat gaatggcctg gcaggctgtc ggccgtatca
      961 ttcgccggac gagtcgaaac cgggtcggcc tgaggctgtt caccaataac aggctctccc
     1021 gacactgcag ccacacgtcc ctcgataatg acaaggtcac tgatttggcg cagcatggcc
     1081 gaggccacgg cttcgtagcg ctcgcggtcc gcctgggccc gcgtcgaatg gatggccggt
     1141 cccttggaga ggttgagggt ccgcatctgg atggcggcct ggtcagtgat gcgccccatc
     1201 aggccgccca gggcatcgat ctcgcgcaca agatggccct tggccgtgcc accgatggcc
     1261 gggttgcaag acatacggcc catggcagcg ggatcgggag taatgatggc caccctgagg
     1321 gggcagcctg acgtcgtcac atcttcgctt tcgggccact ggcatgcccc cggacggcca
     1381 atcctgaccg cagcagccgc cgcctcaatc ccggcatgtc cgccgccgat gacgatgata
     1441 tcgtacctgt ccatccctta ctcctcggcc tgtgaccagt cctcgattgt gatcccggga
     1501 acccgtgcaa actcgcgcac gttgttggta atcagggtca ggccgcgact cctggcctgc
     1561 gccgcaataa aggtatcgat agtcccgatg ggagttccca tcttttcgag cccggcccgg
     1621 atcagggcat agctttcgac atcccggttt ttccagtcaa ggatcctgta gggcaaaagg
     1681 aactggagca gggcaaggta attgcgcctc gggtcactgc ttttgtggca tccatggacc
     1741 agctcggcta cggtacacac cgagacggag agttcccccg
//
LOCUS       DAOSHE010000448         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_67864_length_1776_cov_5.981987, whole genome shotgun sequence.
ACCESSION   DAOSHE010000448 DAOSHE010000000
VERSION     DAOSHE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67864_length_1776_cov_5.981987"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLD82_09925"
     CDS             complement(<1..835)
                     /locus_tag="PLD82_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG65739.1"
                     /translation="MLSPSFIRKVAGKIDRLDHETLREFVRSLAEERDFFQIIFDSMN
                     EGVLITDQAGLVQYSNTTACMLLGKRPENMDGRPVLDCIDSIDFYNLVQNAVYAKERI
                     RNREVSLYIPSDRILNVNIHPLRRSGSIQGHVIIFMDITREKKEQQRLRQAESMSALS
                     SLTAGVAHEIKNPLGAIDIHLQLLQQVLEDGRSARLERDGRRYLSILRDEVARLNTIV
                     VDFLSAVRPIRLKPELVVPADLVNGLAEFVGPALDEAGIALEIRSTPGLPKILADAGL
                     LR"
     gene            complement(835..>1776)
                     /locus_tag="PLD82_09930"
     CDS             complement(835..>1776)
                     /locus_tag="PLD82_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65740.1"
                     /translation="QAVRRLFYRVVNRVNGGYFKPKEATGTAGKKEEDDQVRIAGLER
                     KLDEIPENDAAAAVNAIQGVLEEILEIDELVAHAMLPMAGIQEIIRALSRKPLLGERR
                     VVLIEGIQTFRVEGVNAFLKTLEEPPAGTLILMTCTGLDAVLPTIRSRAAILPFKRPG
                     SALMAAIARDRFGCSQPGDAEGYGDLWEYLESQGEEARAVHGDLARFMDLVRSADRDP
                     EMFEFAKDLEKREAAQVFLQTLADLVAAGLVAAETGHGRERLQAMALSHFRPVFLRRI
                     FREINTLAEGMTRNNFSASQGIVALILAFWLEEQGPR"
BASE COUNT          387 a          553 c          489 g          347 t
ORIGIN      
        1 gcctgagcaa tccggcatct gccagaatct tgggcaagcc gggcgtactc cggatctcga
       61 gcgcgatccc tgcctcgtcc aacgccggcc ccacaaactc ggccagtccg ttgacaaggt
      121 cggcggggac gaccaactcg ggcttgagcc tgatcggccg gaccgcgctc aaaaaatcga
      181 caacgatggt attcagccgg gcaacctcat cgcggagaat cgagagatag cggcgcccat
      241 cacgctccag ccgcgcggag cgtccatcct cgagaacctg ctgcaggagc tggaggtgaa
      301 tatcgatcgc gccgagcgga ttcttgatct cgtgggccac accggccgtc agtgaagaga
      361 gggccgacat gctctcggcc tgccgcaagc gctgctgctc cttcttttcc cgggtgatgt
      421 ccataaaaat gatgacatgc ccctggatac ttccgcttct acgcaaaggg tgaatgttga
      481 cattcaggat ccggtccgag gggatgtaga ggctgacctc gcggtttctg atgcgttcct
      541 tggcatagac cgcattctgg acgagattat agaaatcgat actgtcgata caatcaagca
      601 caggcctccc gtccatattc tcgggacgct tgccgaggag catgcaggct gtcgtgttgg
      661 agtattgcac cagcccagcc tgatcggtta tcaaaacacc ctcgttcatg ctgtcaaaga
      721 tgatctggaa aaaatcccgc tcctcggcaa gactgcggac aaattcccgg agcgtctcat
      781 ggtccaggcg gtctatcttg ccggcaacct ttctgatgaa cgagggcgag agcatcagcg
      841 tggaccctgt tcctcaagcc aaaaggccag aatgagggcg acaatcccct gggatgcgga
      901 gaaattattg cgcgtcatgc cttcagcaag cgtgttgatc tcgcgaaaga tccgccgcaa
      961 aaagaccgga cgaaaatggg agagcgccat ggcctggagc cgctcccggc catgccctgt
     1021 ttcggcagcc accaaaccgg cggcgaccag atcggcgagt gtctgcaaaa aaacctgggc
     1081 tgcttcacgc ttttcaagat ccttggcgaa ttcaaacatc tccggatcgc ggtccgcact
     1141 ccgcaccaga tccataaacc tggccagatc cccgtgaacc gcccgggcct cctcgccctg
     1201 ggactcgagg tattcccaga gatcaccata gccctcggca tctccgggct ggctgcaccc
     1261 gaagcgatcc cgggcaatcg cagccatcaa ggccgaaccg ggccgtttga atggaagaat
     1321 cgcggcccgg gaacgaatcg tcggaagaac ggcatccaga ccggtacacg tcatgagaat
     1381 cagggtcccg gccggtggct cctcgagcgt ttttaaaaac gcattcacac cctcaacccg
     1441 gaaggtctgg atgccctcga tcaggaccac gcgcctctcg ccaaggaggg gttttctgga
     1501 aagcgcccgg atgatctcct ggatccctgc catgggcaac atggcatgcg ccaccaactc
     1561 atcgatctcg agaatctcct cgagcactcc ctggatcgcg ttgacggcag ccgcggcatc
     1621 gttttcggga atctcgtcga gtttcctttc aagacctgca atccggacct gatcgtcttc
     1681 ctcttttttc ccggcggtcc ccgtagcttc tttgggcttg aaatacccgc cgttgacccg
     1741 attgacgact cgataaaaca accggcgcac agcctg
//
LOCUS       DAOSHE010000449         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_68345_length_1768_cov_5.280794, whole genome shotgun sequence.
ACCESSION   DAOSHE010000449 DAOSHE010000000
VERSION     DAOSHE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68345_length_1768_cov_5.280794"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..986
                     /locus_tag="PLD82_09935"
     CDS             438..986
                     /locus_tag="PLD82_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG65741.1"
                     /translation="MATTSAATNREQQRTILARPGKYLTFKLGEELYAVDILKVQEII
                     GMQSVIRIPKTPQYVRGMINLRDRVIPIIDLRLKFDMEHKEDTRRTCIIVLQVSVDTR
                     QVIIGAVVDEVSEVIDLNQDELQPAPSFGSALDTHMIIGIGRTKEKVIMLLDADTLLS
                     TEEIFSLKEVARQAAAQTRPQE"
     gene            999..1541
                     /locus_tag="PLD82_09940"
     CDS             999..1541
                     /locus_tag="PLD82_09940"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65742.1"
                     /translation="MMRGQPTDGHGAGKVFRSRRIAFALVPDEIRSIKKKYRELRIDI
                     PGVALWEIDLKTGGFKPGSGFLEMLSFTPEAMPSRFRDLSPLFPPDDFENVRLRLNEH
                     LLGHTPFFKIRHRLRTSDGLLIWCISVGSVTVRNRDGSPRRIIGSTCAATMYQRNLHR
                     LNRILRALRHERRKARAAVQ"
BASE COUNT          416 a          552 c          466 g          334 t
ORIGIN      
        1 cggatagtgg cctatacgtt accgtatcag gcgcgtcaat gcaatacccg aaacacattt
       61 ttcttcggac acatgatcga ataccaatac actacccata ttgacaaagt tttgtcaagc
      121 ggaatgcgtg cagcctgatc agactttttt tggggaacaa tctccagcca ctggatggcc
      181 gactgttcat caaacaaaat cgtatgtcgc cagcggaaga tccatgcgac cgcgacgaaa
      241 gtggacagga agacccgccg caggcgacag attgacagca gaaaccaatc ttgcccggcc
      301 agatcctgca tctgccggct tcttgctcgt taaagaagcg acgcaacccg caaatcccgc
      361 gaagtctcgt tgacagccga agagaaaggt tctatacttc ggctgtatcc acctactcaa
      421 gtatggaggc gggagtcatg gcgacaacct cagcagcaac aaacagggaa cagcaacgca
      481 cgatcctggc gagaccgggg aaatacctca ccttcaagct gggtgaagaa ctctacgccg
      541 tcgacatcct caaagtgcag gagatcatcg ggatgcagag tgtgatccgc attcccaaga
      601 ccccgcaata tgtccgcggg atgatcaatc tccgagacag ggtcatcccc atcatcgacc
      661 tgcgtctcaa gttcgacatg gagcacaaag aggacacgag acggacctgc atcatcgtgc
      721 tccaggtgag tgtggatacc aggcaggtga tcatcggagc cgtcgttgac gaggtctccg
      781 aggtgatcga tctcaaccag gacgagctcc agccggcacc gtccttcggc tcggccctgg
      841 atacgcacat gatcatcggt atcggtcgga ccaaggaaaa ggtgatcatg ctgttggatg
      901 ccgatacgct cctctcgacg gaagaaatct tctccctcaa ggaagtcgcc cgtcaggccg
      961 cagcccagac ccggccccaa gagtaagcgg ccggtgccat gatgcgagga cagccgactg
     1021 acggacatgg tgcgggaaaa gtgtttcgtt cgcgccgaat cgcgtttgcg ctcgttcctg
     1081 acgaaatccg gagcatcaaa aaaaaatatc gcgaactgcg gatcgacatc cccggggtcg
     1141 cactctggga gatcgatctc aaaaccggag ggttcaagcc gggcagcggt tttctggaga
     1201 tgctcagctt cacacccgaa gccatgccct cccgattcag ggatctctcc ccgctcttcc
     1261 cgcccgatga tttcgagaac gtccgcctcc gtttgaacga acacctcctt ggacacaccc
     1321 cgtttttcaa gatccgccac aggctgcgca cctccgacgg gctcctgatc tggtgcatca
     1381 gcgtagggag cgtgaccgtt cgcaaccgtg atggctcccc acgccggatc atcggctcga
     1441 cctgcgcggc aaccatgtac cagcgcaatc tccaccggct caaccggatc ctgcgtgccc
     1501 tgcgccatga acgccgcaaa gccagagccg ctgtgcaatg acacaagccc gccagccaaa
     1561 ccggcccaca acactggatt gtctccactt ccggcgcagc ccggcatgaa ggtccgcccg
     1621 atgatacccg tcctgatcac aatccttgtc tgctacattg ccatccacgc ctttgtcgga
     1681 cgatcgatcc tgcagagcct gcatgacacg acctgggccc ggagcatcgc cctggccgtc
     1741 ttcgtcctgc tcggatcggg gtggatcg
//
LOCUS       DAOSHE010000450         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_68428_length_1767_cov_2.766939, whole genome shotgun sequence.
ACCESSION   DAOSHE010000450 DAOSHE010000000
VERSION     DAOSHE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68428_length_1767_cov_2.766939"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLD82_09945"
     CDS             complement(<1..377)
                     /locus_tag="PLD82_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOG65743.1"
                     /translation="MAGRVPVLIRVFIVVFLVVVGATLAGFFETLELQLADQRFRLRG
                     TQPVHPEIVHIDIDTDALKAEGNWATWHAGRHAIAFNILRRLGVKALGVDIFFTERGE
                     RRFAVDKLKHTGRQNFSLVEIQRV"
     gene            complement(452..982)
                     /locus_tag="PLD82_09950"
     CDS             complement(452..982)
                     /locus_tag="PLD82_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65744.1"
                     /translation="MRFQISILFIGVGLLVIGACSSSKEAVRPDGGADSSSSATSSTA
                     STARTPAVVTVNNPVFVRDVWSNVRDISAANRSFLTGGSTAVAGVRSYKMSDAQVKEQ
                     AIPRAELEKSVGELRKILADPALGAVDRARCQYYLGACLIQLGDVNNGKRALNEAVRL
                     DRNGRWGRMAAVELSK"
     gene            1118..>1767
                     /locus_tag="PLD82_09955"
     CDS             1118..>1767
                     /locus_tag="PLD82_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65745.1"
                     /translation="MGIRHHSTARHSPRPSAYVLRNARLKRVKKRVRDFFGTVNRKGH
                     EHITLMIASHSANTPVFTLRISKYIILFIAGVLAVTITFSVSAFIEKGTTDPEIERLE
                     RNIERCEETLELFSRNVRTLESSTAKFSDAIKPLAAITGVRTFERMSLFEGASATPGE
                     VHMADTTSDRFGKDIRRLDQINLDILRASQQSSRMNTLIWSRRRISLPNRSLVVSA"
BASE COUNT          412 a          539 c          465 g          351 t
ORIGIN      
        1 acccgctgga tctccaccag ggaaaaattc tgtcgccccg tgtgtttgag cttgtcgacg
       61 gcaaagcgac gttcacctcg ttccgtgaaa aagatgtcca cccccagggc ctttaccccg
      121 agacgcctca ggatattgaa ggcaatggca tgccggccgg catgccaagt ggcccagttc
      181 ccctcggcct tgagggcatc ggtatcgatg tcgatatgga caatctctgg atggacgggc
      241 tgtgtccccc ggagacggaa acgctggtcg gcaagctgga gttccagtgt ctcgaaaaaa
      301 ccggccaagg tagcaccgac aaccaccaga aaaacgacga tgaaaacgcg gataagaaca
      361 ggaacacgac cagccatgag cccccccgag cacatcatca ggcggcatgc accgcctgat
      421 acattgcagt agtggttttg aaaaaacttg tttactttga caactcgact gcggccatcc
      481 ggccccagcg accattccgg tcaaggcgca cagcttcgtt caacgcccgt ttcccgttat
      541 tgacatcacc caactggatc aggcaggcgc caaggtaata ctggcaacgg gcccggtcga
      601 ctgcgccgag cgcgggatcg gccagtatct tgcggagttc accgacactc ttttcgagtt
      661 cggccctggg aatagcctgc tccttgacct gtgcatccga catcttgtag ctgcggacac
      721 cggccaccgc ggttgaccca cccgtcagga agctcctgtt ggctgccgag atatcccgga
      781 cattgctcca gacatcgcgc acaaagaccg gattattgac cgtgacgaca gcaggagtac
      841 gcgccgtgga tgctgttgat gacgtggcgg aagaggaaga gtccgcccct ccatcgggac
      901 ggacagcttc tttggaagag gagcaggcgc cgataacgag caacccaacc cctatgaaga
      961 gaatcgagat ctggaatctc ataacaaggc cccccgggag aaacatgtga ctacctgcac
     1021 gtattctaca ccccttgccc tactccggca acggattcac cgaagtcccg tgtattgaca
     1081 cttgcctgcc cgcccggaac agactaaaat tggcttcatg ggcatacgac accactccac
     1141 agcgcgccac agtccacgac cttcggccta tgttctccgg aatgcccggc tcaagcgggt
     1201 aaaaaaacgt gttcgtgatt ttttcggcac ggtgaaccgc aagggacatg agcacattac
     1261 cctgatgatc gcctcccact cggccaacac ccccgttttt accttgcgta tctcgaagta
     1321 catcatcctc ttcatcgctg gcgtcttggc ggtcaccatc accttttcgg tttcggcctt
     1381 catcgagaaa gggacaaccg atccggagat tgaacgcctg gagagaaata tcgaacggtg
     1441 tgaggagacg ctggagctct tttcacgcaa cgtcaggaca ctggaatcat ccacggcgaa
     1501 attctccgac gccatcaaac cgttggcggc catcacggga gtccggacct tcgagcggat
     1561 gtccctcttt gagggggcgt cggccactcc cggtgaagtc cacatggcgg ataccacaag
     1621 cgaccgattt ggcaaggata tccggcgtct cgaccagatc aatctcgata ttctccgggc
     1681 atcacaacag tcttcgagga tgaacaccct gatttggtcg agacgccgga tatccttgcc
     1741 aaatcgatcg ctcgtggtat ccgccat
//
LOCUS       DAOSHE010000451         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_69086_length_1756_cov_2.503821, whole genome shotgun sequence.
ACCESSION   DAOSHE010000451 DAOSHE010000000
VERSION     DAOSHE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69086_length_1756_cov_2.503821"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PLD82_09960"
     CDS             complement(<1..117)
                     /locus_tag="PLD82_09960"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG65746.1"
                     /translation="MPRQRHLVTSALPYANGPLHFGHIAGAYLPADIYVRYLR"
     gene            complement(298..819)
                     /locus_tag="PLD82_09965"
     CDS             complement(298..819)
                     /locus_tag="PLD82_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65747.1"
                     /translation="MQLVTRIRIFRAVFGLLPFYLSVVLVAAAGVVLGWRGLLLVALP
                     VFLCAGGILWFGLRERGEGIQPGLVARMAVWLMEARREGVMTDLEKLAHVFSATPEQV
                     GEALALVHELALAPFFWDKASGKVHVARTSGSLPHCPWCAVDLPDQPVSGRCKNCHAH
                     YVLTCHKKPFYYI"
     gene            complement(843..1394)
                     /locus_tag="PLD82_09970"
     CDS             complement(843..1394)
                     /locus_tag="PLD82_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65748.1"
                     /translation="MFRRVNEQLPLTPGLRAMLYRPLVYRLTTPDLLLPGAFIASIGM
                     LLTRNWLAGGDQKVAMALLLVSFFVLVLSLNRAAWLLSGRGGINQRRMRFAIDRLLAG
                     GELEILPWAAAAALPLDEVLAELDRYMAEEYHLGYLDTERMRMVLSDQPVVNRRCPVC
                     GAILSDTMVVEKVCGLCGTMLYT"
     gene            1568..>1756
                     /locus_tag="PLD82_09975"
     CDS             1568..>1756
                     /locus_tag="PLD82_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65749.1"
                     /translation="MRKSLWILALVLVSSAIYTAIVVPPTVPPGHIGIIQSDAFGPVE
                     AIVDASGAHLVWSRLIPGD"
BASE COUNT          410 a          527 c          522 g          297 t
ORIGIN      
        1 gcgcaggtag cgcacataga tatcggccgg gagataggcc ccggcaatgt ggccaaagtg
       61 aagcgggccg ttggcgtacg gcagggcact ggttacgaga tggcgttgtc tgggcatggt
      121 tctttcctca tcacgggctt ttccaaagat agccacaaga cccccgccag ggcagtcccc
      181 gttttgaccg gttacagaag cctgatcccg ggttgcccgc attcactgga gtacacagct
      241 ctctgccgga actgaaggct catccgccgg actggagact gttccccgga agaactctca
      301 aatatagtag aatggttttt tgtgacaggt caggacgtag tgggcatgac aattcttgca
      361 tcgtccggag accggttgat ccggaagatc gacggcacac cagggacaat gcggaagact
      421 gccggaggtt ctggcaacat ggaccttccc cgaagccttg tcccagaaaa acggcgccag
      481 cgcaagttca tgcacaagag ccaaggcttc accaacctgc tccggcgtgg cagaaaacac
      541 gtgtgcaagc ttctcgagat cggtcatgac gccctcgcgc cgggcttcca tcagccagac
      601 ggccatcctc gccacaagac cgggctggat accctcgcca cgttcccgca acccgaacca
      661 gagaatcccc cctgcacaca aaaaaacggg tagagccaca agcaacaggc cgcgccagcc
      721 gaggacaacc ccggcagcag ccaccagaac gacggacagg tagaaaggaa gaagcccgaa
      781 aactgcccgg aagatgcgga ttcgcgtgac caactgcatt gaggaggaac tcctgtcatc
      841 gttcaggtgt agagcatggt cccgcagaga ccgcagacct tttcaacgac catggtgtcg
      901 gaaagaatcg ctccacagac ggggcagcgc cggttgacaa ccggctgatc cgagagaacc
      961 atccgcatcc tctcggtatc gagatagccg agatggtact cctcggccat gtagcggtcg
     1021 agttcggcca gaacctcatc caggggaagg gcggctgcgg cggcccaggg cagaatctcg
     1081 agttcgccgc ccgcgagaag ccggtcgatg gcaaaccgca tccgccgctg gttgatcccc
     1141 ccgcgacccg agagcagcca ggccgcacgg ttgagactga ggacgagaac gaaaaaggag
     1201 acaagcagga gcgccatggc caccttttga tcgccacccg ccaaccagtt cctggtcaac
     1261 agcatcccga tcgacgcgat aaaggcaccc ggcaaaagaa gatccggggt ggtcaggcga
     1321 tagacaagag gcctgtagag catcgcccgc aggcccggtg tcagcgggag ctgttcgttg
     1381 actcgtcgaa acatgcttga aatctacatc ttcccggata gaaacggcca tgggcgtatt
     1441 cgaaaggcct gtcccccggg aaaaccatcc ggaaaggact gcagtccgat gggtctcggg
     1501 gtgcgttgac acggaggccc ctgcggggta gacttgagta cagttcacga cggaggggcc
     1561 aaggggcatg agaaaatcac tctggattct ggcgcttgtg ctggtgtcct ctgccatcta
     1621 tacagccatc gttgttcctc cgaccgtacc gcccggccat atcgggatca tccagtcaga
     1681 cgcgttcggt cctgtcgagg cgatcgtcga tgccagcgga gcccatctag tctggtcccg
     1741 cctgatccct ggagat
//
LOCUS       DAOSHE010000452         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_69755_length_1744_cov_4.628774, whole genome shotgun sequence.
ACCESSION   DAOSHE010000452 DAOSHE010000000
VERSION     DAOSHE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69755_length_1744_cov_4.628774"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLD82_09980"
     CDS             complement(<1..695)
                     /locus_tag="PLD82_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65750.1"
                     /translation="MLLRLIRSLFVVLLIFGASLPLASAPLLPSDITRTPWTVFDSPG
                     WMIETGVRDLGMVDGSGFLSASWGGFGFDLFRNQDISGWRFSGGLTLDDGLRAGLSLR
                     LFSGRDWTDSGLDIGLARAFGREVLAEFSIDTIVLSRDAGGRHLPGYRLGLSIGSQSA
                     WFGFEPWVRILPDRDTPEAGTRFVCTPLEGLRLEAEWRYQDEEHRIGLGIGLGLGGLL
                     ARFHGEQRSDTTR"
     gene            complement(680..1417)
                     /locus_tag="PLD82_09985"
     CDS             complement(680..1417)
                     /locus_tag="PLD82_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOG65751.1"
                     /translation="MKLVINADDYGMRASIDAAVESLFELGVLRSASLLVTGASARDA
                     GAFLGAHPGLGGGLHLDLDGIFSSAGFGKDDHGRFLVPDIFFEQDDVRSAIHDRTLAQ
                     LESFKLMTGRTPDHLDGHHHAHLFLPVLQIILPLLQRFRVPAIRWFQGFYPTGWQAGA
                     ARDLIHDAGLATTKLFFDGVLLPGPQEACSAEVMVHPAMPVAAEEKWRAREYAGLADE
                     SFRLRLISSGYEPCTFADLAAAPCCCD"
     gene            complement(1485..>1744)
                     /locus_tag="PLD82_09990"
     CDS             complement(1485..>1744)
                     /locus_tag="PLD82_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG65752.1"
                     /translation="TVQNLPESGPWKYVFANMLPTQFAALACELHDLVEPDGRIIFSG
                     ILREVWADTLAFLEAENLVIERFIDDGKPEGGWAACLARLG"
BASE COUNT          411 a          547 c          508 g          278 t
ORIGIN      
        1 tatcgtgttg tatcggaacg ctgttcccca tgaaaacggg ccaaaaggcc acccagaccc
       61 agtccgattc ccagaccaat cctgtgttcc tcgtcctgat agcgccactc cgcctcgagc
      121 cggagccctt ccaggggtgt gcagacaaaa cgggttcctg cttcaggtgt gtcacgatcc
      181 gggaggatcc gcacccatgg ctcaaatccg aaccatgcag actgcgaacc aatgctcagt
      241 ccgaggcggt atcccgggag gtgacgcccg ccagcgtccc gggagagaac aatcgtatcg
      301 atactgaact cggccaggac ctcccgcccg aaggcccggg ccagcccgat gtcgagcccc
      361 gagtcggtcc agtcccgacc ggagaaaagc cggagcgaga gcccggccct gagtccgtca
      421 tccagtgtca gtccgccgga aaaccgccat cccgatatgt cctgattcct gaagagatcg
      481 aatccgaacc cgccccatga cgccgacaaa aaaccggagc cgtcgaccat gccaagatcc
      541 cggaccccgg tctcgatcat ccagcccgga gaatcaaaga cagtccatgg ggtccgggtg
      601 atgtccgaag ggagaagtgg cgcggaagcg agcggtaggc tcgctccgaa aatcaggaga
      661 acaacgaaaa gagagcgtat caatcgcagc agcatggggc agccgccaga tcggcaaagg
      721 tacacggttc gtacccggag gagatcaacc gcaaccggaa ggactcatcg gccagaccgg
      781 catactcgcg cgcccgccat ttttcttcgg cggccacagg catcgcagga tggaccatca
      841 cctctgccga acaggcttcc tgcggaccgg gcaggaggac accatcaaaa aacagctttg
      901 tcgttgccag tccggcatca tggatcagat cgcgggccgc accggcctgc cagcctgtcg
      961 gatagaatcc ctggaaccag cgaatggccg ggacccggaa ccgctgaagc aatggaagaa
     1021 tgatctggag aacgggaaga aagaggtggg cgtggtggtg gccatcgaga tggtccggcg
     1081 tccggcccgt catcaatttg aagctctcca actgggccag tgtcctgtca tggatcgccg
     1141 agcgcacgtc atcctgctcg aaaaaaatgt ccggaacaag gaaacggcca tggtcgtcct
     1201 tcccgaaccc ggcggaggaa aaaatgccgt caaggtcgag atggagacct ccacccaaac
     1261 cgggatgtgc ccccagaaat gcgccggcgt cccgggccga agccccggtg accaaaaggg
     1321 aagcgctccg taagaccccg agctcgaaca ggctttcgac cgctgcatcg atggatgccc
     1381 gcattccgta gtcgtccgca ttgatgacca gcttcatccg gcacacccca catggattct
     1441 cgtatcccct aaagatcatt ccggggatgg tgcatgggca agccctatcc aagccgtgcc
     1501 aggcaggcgg cccagccgcc ttccggtttg ccgtcatcga taaaacgctc gatgacgaga
     1561 ttctctgcct cgaggaaggc cagggtatcc gcccagacct cgcgtaaaat gcccgaaaag
     1621 atgatcctcc cgtccggttc gacgagatcg tggagctcgc aggccagggc agcaaattgc
     1681 gtgggcaaca tatttgcaaa aacgtatttc cagggaccgg actcgggcag gttctggaca
     1741 gtgc
//
LOCUS       DAOSHE010000453         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_70180_length_1737_cov_3.118906, whole genome shotgun sequence.
ACCESSION   DAOSHE010000453 DAOSHE010000000
VERSION     DAOSHE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70180_length_1737_cov_3.118906"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>1737
                     /locus_tag="PLD82_09995"
     CDS             143..>1737
                     /locus_tag="PLD82_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65753.1"
                     /translation="MKKIMLLVLLVVCTAFPLSSALPHHSVTRDCAIFIQIDRIPALI
                     AEIERYAISFGLGLSPGDLSKKISGGIFGQDGFPGIDLNKPLAVFLLHDGQKMSGGVV
                     MVPVSDQNLFASFVLSKIPLGNAKLQYRDGYALIASEAGVMDRFVRGQKKPVTLVPGA
                     QVSFYMDMALFKDEIRKLVAMYDTRAGGTEGKLISSVLRLYSDVLQEMQGVTYALSLE
                     SRGLELGYAIDLKAGGKMARVLAATPTGSPDVLGRMPADSYLAIGSRMNLQASLPLVE
                     PVLSTFNGIQPELGTILKDFFRDAARLYGDDYAMALVPSKTSGFTMVGAVKQAGASTQ
                     TLYHKLVQRLNGLRFVQRLKGEDARFGLVHERSAGTVAGVSYDTIRLDFTDPEDDREE
                     TRKQVALVREIFVCHVARKNGVEYWAMGTDAKNRLAAMLDGSAAGFRSSEAWRTMTAA
                     WGAYSKNGLMYFSLPGLLKEATALAKRLDGAGSRIAAIERFLAGVQPDGGIFMMTKYA
                     NNSMTVRGLVTRGEIQAVAQFVL"
BASE COUNT          358 a          450 c          513 g          416 t
ORIGIN      
        1 gagttctctc tgaagtgaac ccctcgccga aggactcatg agcttttctt tgtgtgtggt
       61 gtatacttct ccgtgattga tcaagaacaa gtcaaagccg ggccagtccg gtcgggcttc
      121 tcaaaagaaa ggatatccag cgatgaaaaa gatcatgctt cttgtgcttc ttgtcgtgtg
      181 tacggcattt ccgctttcgt cggcccttcc gcatcactca gtcacccggg attgtgccat
      241 ttttatccag atcgacagaa ttccggctct gattgccgaa atcgagagat atgccatctc
      301 cttcgggctt ggtctctctc cgggcgacct ttccaaaaag atttcaggcg ggattttcgg
      361 gcaggacggg ttccccggaa tcgacctcaa caaaccgctg gccgtctttc tcctccatga
      421 tggacaaaag atgagtggcg gagttgtcat ggtgccggtc agtgaccaga atctctttgc
      481 cagctttgtc ctctccaaga ttccgctcgg aaatgccaag cttcaatatc gggacggata
      541 tgccctgata gccagtgagg cgggtgtcat ggacaggttt gtccgtggcc aaaaaaaacc
      601 ggtcaccctt gtgcccggtg cacaggtttc cttctatatg gatatggctc ttttcaagga
      661 cgagatccgc aagcttgtcg cgatgtatga tacccgcgcg ggaggtaccg aagggaagct
      721 gataagttcg gtgttgcggc tgtattccga tgtgctccag gagatgcagg gagtgacgta
      781 cgccctctcg ctcgagtcgc gcgggttgga actgggctat gccatcgatc tcaaggcagg
      841 tggcaagatg gccagagttc tggcagccac cccgacaggc agtcccgatg tgttgggcag
      901 gatgccggcg gacagttatc tcgccatcgg ttcacgcatg aaccttcagg cgagcctgcc
      961 cttggtcgag cctgtcttga gcacattcaa cgggatccaa cccgaattgg gcaccatcct
     1021 caaggatttt ttcagggatg ccgcgcgtct gtatggagat gactacgcca tggcgcttgt
     1081 cccgagcaag acttccgggt ttacgatggt cggagcggtc aagcaggcgg gcgcttcgac
     1141 acaaacactc tatcacaaac ttgtgcagcg gctcaacggc ctgcgttttg tgcaacggct
     1201 caaaggtgag gatgcgcggt tcggtctggt tcatgaacgt tctgccggga ctgtggctgg
     1261 tgtgtcctac gataccatcc gccttgattt taccgatcct gaagacgaca gggaggaaac
     1321 gagaaaacag gtcgccttgg tgcgcgagat cttcgtctgt catgttgccc gcaaaaacgg
     1381 tgtggagtat tgggcgatgg ggacggatgc caaaaacagg ctcgcagcca tgctggacgg
     1441 gagtgccgcc ggtttcaggt catccgaggc ctggcggacg atgacggcag cctggggggc
     1501 ttattccaaa aacgggttga tgtatttctc tcttcccggc ttgctcaagg aagccacagc
     1561 tcttgccaaa cgtcttgatg gggccggttc acggattgcc gcgatcgaga gattcctggc
     1621 aggggtacag ccggatggag gcatcttcat gatgacgaag tatgccaata attccatgac
     1681 ggttcgtggg ttggtgacca ggggcgagat ccaggccgtc gcccagtttg tcctttt
//
LOCUS       DAOSHE010000454         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_70527_length_1731_cov_3.616348, whole genome shotgun sequence.
ACCESSION   DAOSHE010000454 DAOSHE010000000
VERSION     DAOSHE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70527_length_1731_cov_3.616348"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1112
                     /locus_tag="PLD82_10000"
     CDS             114..1112
                     /locus_tag="PLD82_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG65754.1"
                     /translation="MKARTVLWPATPVILLLALFAGSTDVAVSDILRGFVGEADTAAR
                     AALRTVLEFRVPVLVQAFCVGGLLSISGAALQAVLQNPLAEPYILGTSSGAAFGFSLA
                     GAVGLAPFFWPRTLLSFVCALAASALVIASGWRRGRGLSPLALILGGVVISAFLSAAT
                     IAVQSLLTPFEFRNTIGLLLGGFRIIPTEELFLTLVPALMAGLVLILRAPVLDILSTG
                     IPAARSVGLAAGRELAGVVLLVTLITALTVPLAGIIGFVGLVAPHLMRLGGRTRHARL
                     LPAAFAAGGLMTVAADALVRQASSLGQLPAGAVTAMLGAPVFIVLLRRYGGFHDRM"
     gene            1099..>1731
                     /locus_tag="PLD82_10005"
     CDS             1099..>1731
                     /locus_tag="PLD82_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG65755.1"
                     /translation="MIECKSLAWRAGAFALGPLEYGFRPGCTVILGPNGSGKSSLLAL
                     IAGLVRPDSGSVFCDSQDICRLSNKARAGLIAFAPQLLQAPPGLCLADFVSMGRYRFT
                     GGSGRAIYGDLASRTAVRDMLALVGLGGCADADLSVLSGGEQRLAILARALVQEADWT
                     LLDEPAGFLDYAHNAVLHGLLAREKRAGHNLILVTHDADFAASIADSVICL"
BASE COUNT          214 a          557 c          540 g          420 t
ORIGIN      
        1 gaggcgttct ggaaacgata tgcaccccgg gcccgtctcc tcgcactgga tcccgccctc
       61 tttgagcagc ccggcccccg gatcgccgaa ggaatccagg cgttggcccg accatgaagg
      121 cccggaccgt tctctggcca gcgacgcccg ttatcctcct gctggccctg tttgcaggaa
      181 gcacggatgt ggcggtttcg gacatcctgc gtggttttgt cggggaggcg gacactgctg
      241 cccgggccgc tctccggacc gtcctcgagt tccgggtacc tgttcttgtc caggcttttt
      301 gtgtcggcgg tttgctctcg atctccggcg ccgccctcca ggctgtcttg caaaatcctt
      361 tggccgaacc atacatcctg gggacttcca gtggcgctgc cttcggattc tccctggccg
      421 gggccgtcgg actcgcacca tttttctggc cacggacact tctttcgttc gtgtgcgctc
      481 ttgcggcctc ggcactcgtg atcgcctccg gttggcgccg cggacgcgga ctctccccgc
      541 ttgcgctgat cctcggaggg gttgtgatca gtgctttttt atctgcggcc acgatcgcag
      601 tccagtccct tttgacgccg tttgaattcc ggaatacaat cggcctgctc ttgggcggct
      661 ttcgcatcat ccccacggaa gaactcttcc tgacgctggt tcccgccctg atggccggac
      721 tggtcctgat cctgcgggcg cccgtgcttg acatcctctc cacgggaatc cctgcagcgc
      781 gctcggtcgg tctggccgcc gggcgcgagc tggcgggtgt ggtgctcttg gtcaccctga
      841 tcacggcgtt gaccgttccg ctcgcgggca tcatcgggtt tgtcggactc gttgcgcccc
      901 atctcatgcg gctcgggggg agaacccggc acgcacgctt gctgccggcc gcgtttgccg
      961 cgggcggcct gatgacggtt gcggccgatg cccttgtccg gcaggcttcc tcgctcggcc
     1021 agcttcccgc gggagccgtg actgccatgc tcggggcgcc ggtgtttatc gtcctgctgc
     1081 ggcgttatgg tggtttccat gatcgaatgt aaatcgctcg cgtggcgagc cggtgccttt
     1141 gcgcttggcc cgctcgagta cggcttccgc cccggatgta ccgtcatcct tggtccgaat
     1201 gggtccggga agtccagtct cctggccctg attgcagggc tggtccgtcc tgactccggc
     1261 tctgttttct gtgattcgca ggatatttgc cgtctctcaa acaaggcacg agccggcttg
     1321 atagcctttg ctccccagct gcttcaggcg ccacccggat tgtgccttgc ggattttgtc
     1381 tccatgggcc gctatcgctt taccggagga agcgggcggg cgatctatgg ggatctagca
     1441 tcgcggaccg ccgtccggga tatgcttgca ctggttggtc ttgggggctg tgctgatgcc
     1501 gatctttcgg tcctttcagg cggggaacag cgtctggcca ttcttgcccg cgcgctggtc
     1561 caggaagcgg actggacact gcttgacgaa ccggcgggtt ttttggatta tgcgcacaat
     1621 gccgtgctgc acggtctctt ggcccgtgaa aagcgggccg ggcacaacct gattctcgtg
     1681 acccatgatg cggatttcgc cgcatcgatt gccgattccg tcatctgtct g
//
LOCUS       DAOSHE010000455         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_70790_length_1727_cov_4.290072, whole genome shotgun sequence.
ACCESSION   DAOSHE010000455 DAOSHE010000000
VERSION     DAOSHE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70790_length_1727_cov_4.290072"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1727
                     /locus_tag="PLD82_10010"
     CDS             12..>1727
                     /locus_tag="PLD82_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOG65756.1"
                     /translation="MDSLDQELDEHAFSAFGPFPWKRSTYARPLEKFSQGQLTISLSW
                     NGIQDPAHARYCVYQSESPIRTTDDLAKATLSRTIASADEQIFSDILIVKPGRWYFAV
                     TSVDTNGVENRHIAFGTNATKFPVSIQVKPALAADAQQWDKQYQLGMIREQPKSSVTN
                     NTANLIGGLYAILNYPEAVFFDVFMLDHKDKDDDQILINAFSKNSSIFVDYKLNDDNT
                     SPNPPDFTRRIQALQRHTIAADQTHAKRSEIHAPGVALPIPEVIESVDGAGFANAVRD
                     ADETIRSWKLVSQFESDKFPNRYYPSAPLLLAMKYFRVPPEKVRILPGESVTLMDAEY
                     RIRDKEGKITHIERKDLVIPIDSNGVLDIHYIGPHGTFVEHQDELSSFASFEKYSLSS
                     LSDKIFMIGLFELAIKADDKTTDYWLTPVGQMFGIEVMANTLNTIITGNFLQRASSQT
                     NALVVFLVSILLALVLAKLSTLRGAIFALAITLLFIIVAYLLYTENFIINMSMPLAAV
                     FFTFLGTTIYKTLTEEAEKKYIRTQFGKFVNKNVVDELLKDPSKLRLGGERRSMTVLF
                     SDIRGF"
BASE COUNT          443 a          469 c          390 g          425 t
ORIGIN      
        1 tagagatcca gttggattca ctcgaccagg agttggacga gcacgcgttt tccgcttttg
       61 gcccatttcc ctggaagcgc tcaacatatg cgaggcccct tgaaaaattt tcacaggggc
      121 agttgacaat atccctgtca tggaacggta tccaggatcc ggcccatgcc aggtactgtg
      181 tctatcaaag cgaatcgcca atcaggacca cggatgatct ggcaaaagcg actctctcca
      241 ggaccatagc ctctgccgat gaacagattt tcagtgacat cctgatagtc aaaccgggac
      301 gctggtattt tgccgtcacc agtgtcgata ccaacggtgt cgaaaaccgg catattgcct
      361 tcgggacaaa tgcaaccaaa ttcccggtca gtatccaggt caaacctgcg ctggcagcgg
      421 atgcccaaca gtgggataaa caatatcagc tcggtatgat tcgcgaacag ccaaagtctt
      481 cagtcaccaa taacacagcg aacctgattg gcgggctgta cgccattctc aattatcccg
      541 aagcggtttt tttcgacgtg ttcatgctgg atcacaagga caaggatgat gatcagatcc
      601 tcatcaatgc gttttcaaaa aacagttcca tctttgttga ctataaactc aacgatgaca
      661 acacaagtcc caatcctcct gacttcacaa gacggattca ggcactccaa cggcacacca
      721 tcgccgctga ccagactcat gccaagcgga gtgaaatcca tgcaccaggg gttgctctgc
      781 ctatccccga agtgattgaa tctgttgacg gtgccgggtt cgccaatgca gtccgtgatg
      841 ccgacgaaac catccgttca tggaaacttg tctcccagtt tgaaagcgac aaattcccga
      901 accggtacta cccgtcagcc ccacttttac tggccatgaa gtattttcgt gttccacctg
      961 aaaaagtccg aatcctgccg ggggaatccg tgactctgat ggacgcggag taccgcatcc
     1021 gtgacaaaga aggcaagatc acgcacatcg agcggaagga tctggtgatt cccattgatt
     1081 ccaacggcgt tcttgacatc cattacatcg ggccccatgg tacatttgtc gagcaccagg
     1141 atgagctttc ctctttcgcc agcttcgaga aatactcgct cagcagcctt tcggacaaga
     1201 ttttcatgat tggtctcttc gaacttgcca tcaaggccga cgacaagacg actgactact
     1261 ggctcacacc tgttggccag atgttcggga tcgaagtcat ggccaacaca ctcaacacga
     1321 tcatcaccgg aaatttcctc caacgggcat cgtcacagac gaatgcattg gtcgtcttcc
     1381 tggtttcaat tctccttgca ctggttttgg ccaaactctc gactctcagg ggggcaatct
     1441 ttgccctcgc tataacgctt cttttcatca ttgttgcgta tctcttgtat acagagaatt
     1501 tcatcatcaa catgtccatg cctttggcag cagtcttctt tacttttttg ggaacaacca
     1561 tctacaaaac gttgaccgag gaagctgaaa aaaagtatat ccggacccag ttcggaaagt
     1621 tcgtcaataa aaatgtcgtt gacgaattgc tcaaagatcc ttccaagctc agattgggtg
     1681 gagaacgccg ctcgatgaca gtcctttttt cggatatccg gggattc
//
LOCUS       DAOSHE010000456         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_71397_length_1717_cov_3.356799, whole genome shotgun sequence.
ACCESSION   DAOSHE010000456 DAOSHE010000000
VERSION     DAOSHE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71397_length_1717_cov_3.356799"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..783)
                     /locus_tag="PLD82_10015"
     CDS             complement(175..783)
                     /locus_tag="PLD82_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65757.1"
                     /translation="MRAQALFDQMVMDGGRGEERGDGRVVTVGLPVGENEDIRPLLDN
                     LGSVDAEFLEGLLKGVIPSIRRKDGGQDNGLEVGPCRGFDPGQVVIGQNRVREFEFAA
                     MLGALVQQVPVATDKAREGHHETFSDWVDRRIGDLGKKLFEIIEEQLRLIGEDSERGI
                     GSHRADRFLPVTGHRAQDVFDLFIAVPVQDLLGAQEFLVGSG"
     gene            1002..>1717
                     /locus_tag="PLD82_10020"
     CDS             1002..>1717
                     /locus_tag="PLD82_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG65758.1"
                     /translation="MGISTLRSYQSAQIFEAIGLAPALVERYFTNTPSRIGGIGLREI
                     QQETLARHRQAWAPNRVTELLPIGGQHRVRVKTENHLWTPQTIRDLQLAVINNDLSRY
                     RDYAHWINEQEGHTCTLRGLFRFKAGNAVPLDEVEPATAIMKRFATGAMSFGSISREA
                     HESLAIAMNMIGGKSNTGEGGEEARRFLPRPDGTSARSAIKQVASARFGVTAAYLVNA
                     DEIQIKMAQGAKPGEGGQLPG"
BASE COUNT          392 a          590 c          455 g          280 t
ORIGIN      
        1 cgcatcatca aaaaaggccg cctcatgccg ggcaagatgt tcgtcattga caccgtcgaa
       61 gggcgcctga tcgatgacaa cgagatcaaa tcccggatca gccgccaaaa accctaccgc
      121 cactggctcg accggaaccg gatcgaactc aagggactct tcggaacacc gacatcagcc
      181 cgacccgacc aggaactcct gcgcaccaag cagatcctgc acgggtacag ctatgaacag
      241 atcgaacacg tcctgcgccc gatggcccgt gacgggcagg aacctgtcag ctcgatggga
      301 accgatgcct cgctcgctat cctctccaat gagccgcaac tgctcttcaa ttatttcaaa
      361 cagctttttg cccaggtcac caatcctccg atcgacccaa tccgagaagg tctcgtgatg
      421 accctcacga gctttatcgg tcgcgaccgg aacctgctgg acgagagccc cgagcattgc
      481 cgcaaactca aactcccgca cccgattctg accaatgacg acttggcccg gatcaaagcc
      541 tcgacaaggt ccgacttcaa ggccattgtc ctgcccgccg tctttccggc ggatggaggg
      601 gatcacgccc ttgagcaggc cctcaaggaa ctctgcatca acgctgccca gattgtcgag
      661 cagggggcgg atgtcctcat tctctccgac cggaaggccg accgtgacca cgcgcccatc
      721 ccctcgctcc tcgcctcggc cgccgtccat caccatctga tcaaaaaggg cctgcgctcg
      781 caaaccggcc tcattgtgga atcggctgaa ccccgggaag tgatgcactt tgccctcttg
      841 gcggatacgg cgtcaacgca atcaatccct ggctggcctt cgagaccatc acatccctgg
      901 ccgatcgggg agccctcggc gatgagatcc cggacagcga cgaagcaatc gaccattaca
      961 tcaccgccat caaaaaaggg cttctcaaga tcttttcgaa gatggggatc tccacactcc
     1021 gctcctacca gagcgcccag atttttgaag ccatcgggct ggcccccgcc ctggtcgaac
     1081 gatatttcac caacacgccc agccggatcg gcgggatcgg cctgcgcgag atccaacagg
     1141 agaccctggc ccgccaccgc caggcctggg cgcccaatcg tgtcacggaa cttctcccga
     1201 ttggcggcca acaccgggtc cgggtcaaga ccgaaaacca tctctggacc ccgcagacca
     1261 tccgggatct gcaactggcc gtcatcaaca acgacctgtc ccgataccgc gactacgccc
     1321 actggatcaa cgaacaggaa ggacacacct gcacactgcg gggactcttc cgtttcaaag
     1381 cggggaacgc tgttcccctt gacgaagtcg aaccagccac ggccatcatg aagcgtttcg
     1441 ccaccggcgc catgtccttc ggatcaatca gccgggaagc ccacgagtcc ctggccatcg
     1501 cgatgaacat gatcggagga aagagcaaca caggagaagg cggcgaagaa gcacgccgct
     1561 tcctgccaag gccggacggg accagcgccc ggagtgccat caagcaggtc gcttcggcac
     1621 gatttggcgt gaccgccgca tatcttgtga acgccgacga gatccagatc aagatggcgc
     1681 agggcgccaa gcccggcgag ggcggccagc tcccggg
//
LOCUS       DAOSHE010000457         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_73510_length_1684_cov_4.062615, whole genome shotgun sequence.
ACCESSION   DAOSHE010000457 DAOSHE010000000
VERSION     DAOSHE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73510_length_1684_cov_4.062615"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLD82_10025"
     CDS             <1..899
                     /locus_tag="PLD82_10025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG65759.1"
                     /translation="PWEVFNTNALEYYGMINLLKGGILFADRLNTVSASYANSIRTTN
                     AGCGLEGVLSSRSDALSGIMDGIDYTVWDPATDPWLGDLRFAEDSLEHKLLLRANLLR
                     QLGIESKPGMALLVCASRLEPQKGMSLFEAAREQLMSRNVALVIASSGTRRYTELARR
                     MVELFPGRVAFSEEIGSSPLVHQAIAAADIILLPSEYEPCGLWQFVAMRYGTLPLARR
                     TGGLADSIEDDTTGFLFDEYRDSAFLSALDRALAAFSDTEAWSSMQRKAMEQDWSWTH
                     SARQYIGIYKELATPCNEPENT"
     gene            880..>1684
                     /locus_tag="PLD82_10030"
     CDS             880..>1684
                     /locus_tag="PLD82_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG65760.1"
                     /translation="MNPKTRESLLRSLDIIIALPVITGSALLLFAGHDPGRYASELAD
                     LDTVLTILLVVDLFLRIFLRGSLLRFFKHDWLALGASTSFCAIRFLMPGDTLRIALLE
                     ISPDSLAVLASLLRVAVLLIRGIPTLMSIRDVFSNMRTQPARAVISGFAAVILAGAVL
                     LSLPQSHNPGQTPSFLDALFTSTSAVCVTGLAVVDTGTTYSVFGQFILLLLIQVGGLG
                     IMTLAVFLALLVRNRVSAREQYTALSALDAGRSDNLRTLVRQILRFTLVI"
BASE COUNT          292 a          568 c          432 g          392 t
ORIGIN      
        1 ttccctggga agtcttcaac acaaacgccc ttgaatacta cgggatgatc aatcttctca
       61 agggtggcat cctctttgct gaccggctca acactgtcag cgccagttat gccaacagca
      121 tccgcaccac caatgccggc tgtggcctgg aaggtgtgct ctcctcacga agcgatgctc
      181 tctccggaat catggatggc atcgattaca cagtctggga tccggcaacg gatccctggc
      241 ttggagatct gcggtttgct gaagacagcc tcgagcacaa gctcctcctg agagccaacc
      301 ttctccgaca gctcggtatc gaaagcaaac ccggcatggc gctcctcgtc tgtgcatcca
      361 gacttgagcc acaaaaaggg atgtccctct tcgaagccgc ccgcgagcaa ctgatgagcc
      421 ggaatgtcgc cttggtcatc gcgagctccg ggacaaggcg ctacaccgaa ctcgctcgcc
      481 ggatggtgga actcttcccg ggtcgggtcg cgttttccga agagatcggc tccagccctc
      541 tggtccatca agccatcgcg gccgccgaca tcattcttct gccgtccgaa tacgagccct
      601 gcggactctg gcagtttgtt gccatgcggt acggaaccct gccgctcgcc cgacgcaccg
      661 gtgggcttgc tgacagcatc gaagatgata ccactggctt cctgtttgat gaataccggg
      721 acagcgcctt cctctcggcc ctcgatcggg cgctcgcggc attctccgac actgaagcct
      781 ggtcctccat gcagcggaaa gccatggagc aggactggtc atggacgcat tccgcccgcc
      841 agtatatcgg tatctataaa gaactggcga cgccctgcaa tgaacccgaa aacacgtgag
      901 tccctgctcc gttctctcga tatcatcatc gcgcttcctg ttatcaccgg aagcgcgctc
      961 ctcctctttg ccggacacga ccccgggcgc tatgcctcgg aactggccga tcttgacacg
     1021 gtcctgacca ttcttcttgt cgttgatctc ttcctgcgaa tctttctccg aggatctctc
     1081 ctccggtttt tcaaacacga ttggctcgct cttggtgcaa gcacctcttt ttgcgccatc
     1141 cgttttctga tgcctggcga caccctgcgc attgcactcc tggagatcag tcccgactcc
     1201 ctggccgttc tggcctccct cctgcgggtg gccgttctcc tgatccgtgg catcccgacc
     1261 ctgatgtcga tccgggacgt tttctccaat atgcggacac agccggcccg ggccgtcatc
     1321 tcgggctttg ccgcagtcat tctggccgga gccgttctcc tctccctgcc ccagtcccac
     1381 aatccgggac agactccgtc attcctggat gctctcttta catccacctc ggcggtctgt
     1441 gtgaccggat tggccgttgt cgataccgga acgacctatt ccgtcttcgg tcaattcatc
     1501 ctcctgctcc tgatccaggt cggaggactg ggcatcatga ccctggcggt ttttttggcg
     1561 ctattggtcc gcaaccgggt ctcggcccgc gagcagtaca ccgcactttc ggccctggac
     1621 gccgggcgct cggacaatct gcgcaccctc gtccgccaga tcctgcgctt tacccttgtc
     1681 atcg
//
LOCUS       DAOSHE010000458         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_73788_length_1680_cov_3.193231, whole genome shotgun sequence.
ACCESSION   DAOSHE010000458 DAOSHE010000000
VERSION     DAOSHE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73788_length_1680_cov_3.193231"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="PLD82_10035"
     CDS             complement(<1..1240)
                     /locus_tag="PLD82_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HOG65761.1"
                     /translation="MNSPGLYRPEYEHDNCGVGFVANIDGRRDHAIIEHGLSVLENLI
                     HRGAVGGDGKTGDGAGMLVQLPDAFFRRVLALDRVRLPPQGEYGAGMLFVERTGDAAS
                     LGHKALALAAEELGLPWIGLRTVPTDPDCLGELALAGMPHILQFFVGLPSGVRPGDDF
                     ERDLYLMRRTAEKKMAAAGFGRNAWYVCSLSSRTIVYKGLFVGAQIRQFYPDLSAPDF
                     ASCLALVHQRYSTNTFPTWSLAQPFRFVAHNGEINTLRGNINKMRARENTMSSPLFGE
                     DIERIRPVIDPDGSDSAVFDNVYELLTRSGRSIEHAMMMMIPEAFGVKYHISQDKRAF
                     YEYHANFMEPWDGPAAMVFTDGIRIGATLDRNGLRPCRYTLTSDGFMVLASECGVLEI
                     EPSRIIKKGRLMPGKMFVIDT"
     gene            1410..>1680
                     /locus_tag="PLD82_10040"
     CDS             1410..>1680
                     /locus_tag="PLD82_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG65762.1"
                     /translation="MFERVYGQMRIRTVGRVLREFESVDSTNRIARDLAAEGEAEGTV
                     VVSRQQTAGRGRLGRDWVSPLDGGLYFSVILRPEIGEIAARERLST"
BASE COUNT          331 a          446 c          552 g          351 t
ORIGIN      
        1 cggtgtcaat gacgaacatc ttgcccggca tgaggcggcc ttttttgatg atgcgggagg
       61 gctcgatctc cagaacaccg cactcggacg ccaggaccat gaagccatcc gaggtcaggg
      121 tgtaccggca gggtctcagt ccatttctgt ccagtgtggc cccgatgcgg attccgtcgg
      181 taaagaccat cgcggctggt ccgtcccagg gttccatgaa gttggcgtgg tactcgtaaa
      241 acgcccgctt gtcctgactg atgtggtatt tcacgccaaa ggcctcgggg atcatcatca
      301 tcatggcgtg ttcgatcgaa cggccggagc gggtcaggag ttcgtagacg ttgtcaaaga
      361 cggcggaatc gcttccgtcc ggatcgatga cggggcggat tcgctcgatg tcctccccga
      421 acaacggaga ggacatcgtg ttttcgcggg cccgcatctt gttgatgttg ccgcgcaggg
      481 tgttgatctc gccattatgt gccacaaagc ggaatggctg ggccagcgac caggtcggga
      541 aggtgttggt gctgtagcgt tggtgtacca gggccagaca gctggcgaaa tcgggggccg
      601 agagatccgg gtagaactgg cggatctggg ccccgacaaa gaggcccttg tagacgatcg
      661 tccggcttga gaggctgcag acataccagg cattccggcc aaaaccggcc gcagccatct
      721 ttttttcggc ggttcggcgc atcaggtaga ggtcgcgttc gaaatcgtcg ccggggcgta
      781 ctccggatgg aagcccgacg aaaaactgga ggatgtgggg catcccggcg agggccagtt
      841 cgcccaggca gtctgggtcg gtgggaacag tcctcagacc gatccagggg aggccaagtt
      901 cttcggcggc caaagccagg gccttgtgtc cgaggcttgc ggcatctccg gtgcgctcaa
      961 caaagagcat gcccgccccg tactccccct gcggagggag ccggaccctg tccagggcca
     1021 aaacccggcg gaagaaggcg tcgggcagct ggacaagcat gccggctccg tcgccggtct
     1081 tgccgtctcc cccgacggct ccgcgatgga tgaggttttc gagaacggag aggccgtgtt
     1141 cgatgatggc gtggtcacgg cggccatcga tattggcgac aaagccgacg ccacagttgt
     1201 catgttcgta ctcaggtcga tagagaccgg ggctgttcat gcatcctcct tggggagtta
     1261 cgagcatctg cttaattttt aagcagatgc ttaaaaatta agcaacacat ttactccacg
     1321 gtgaaaattt accattcccg gccaagaatg aaagaaatta attgtttttt cttcatgact
     1381 agatttaggt gggtttcgcg ggaggaatcg tgttcgaacg agtgtatggg cagatgcgga
     1441 tccggacagt gggcagggtt ttgcgcgaat tcgagagcgt cgattccact aaccgcatcg
     1501 ctcgcgatct ggcggccgaa ggtgaggcgg aagggaccgt ggttgtgtcc cgacagcaga
     1561 cagccggccg gggccgactt ggccgggact gggtctctcc tcttgatggg gggctctatt
     1621 tcagtgtgat tctccggccg gagattggcg agattgcggc ccgggaacgg ctttcaactg
//
LOCUS       DAOSHE010000459         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_74188_length_1675_cov_2.182716, whole genome shotgun sequence.
ACCESSION   DAOSHE010000459 DAOSHE010000000
VERSION     DAOSHE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74188_length_1675_cov_2.182716"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLD82_10045"
     CDS             complement(<1..128)
                     /locus_tag="PLD82_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG65763.1"
                     /translation="MKQEAENGMKLLRVENLVKMYKKRTVVNKASLEIRQGEIVGLL"
     gene            complement(125..>1675)
                     /locus_tag="PLD82_10050"
     CDS             complement(125..>1675)
                     /locus_tag="PLD82_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65764.1"
                     /translation="RADFVTYDTGKDIISWRGSTMAVVGTAAQAKKETDWASQNNASV
                     FTLSTGANGANVIKVVGDVWLSTSGRAVTNKGQVSWGKRADDADSIPSGTHIVINDTW
                     SWIDMLGRDGLMLRLRGPAHLVLRSAAWENPPRPTNERPAIRTNTDTRASSAPRGKTV
                     GASSSRAASPALHGEHFPASQSASSARSVSSTALVLTNMKIASEITNAVSQRTGKDSA
                     PLMHAGTNQDPDGSTNRQARTDQQVARNPRRTGRSLWDLRRGIILARPGAGAVLDLAA
                     GVFSCSNLAVPLVAEAGTNGGLRILALSNQQNVQGRIGIPALRLVTFRSNSEILDFRK
                     KNDPVLSMTIAPDRPLVVRTGNILDLSAIMETRLSNELFAKNLVLSNANFTNTNARAG
                     MEPDESILSNRTLIMLPGVEVVRTFYEGLTNVAATNTPGQPVVFAPFSRVNVTAPLQA
                     RILWEGATATWISETLAGQEFWKTEQLPGKYTEYGELIEEARGLSISWGRRPTTGPQP
                     DRKQGGTR"
BASE COUNT          309 a          532 c          496 g          338 t
ORIGIN      
        1 agaagaccga caatctcccc ctgacggatt tccagggagg ccttgttgac cacggtcctc
       61 tttttgtaca tcttgaccag gttctccacc cgcaggagct tcatcccgtt ctccgcctct
      121 tgcttcatcg cgttccccct tgcttcctgt cgggctgcgg tcctgtcgtt ggacgtcttc
      181 cccagctgat cgaaagcccc cgggcctctt cgatcagctc tccgtactcg gtgtacttgc
      241 ccgggagctg ttccgtcttc cagaattcct gcccggccaa ggtctcactg atccaagtgg
      301 cggttgcccc ctcccagagg atccgtgcct ggagtggggc ggtcacattc acccgactga
      361 aaggagcgaa cacgaccggt tgaccgggcg tattggtcgc cgccacattg gtcagaccct
      421 cgtaaaacgt ccggacgacc tcgacaccgg gaagcataat cagggtacgg ttggagagga
      481 tactttcatc cggctccatg ccggccctgg cattggtgtt ggtgaaattg gcgttcgaga
      541 ggacaagatt cttcgcgaag agttcattgg aaagcctggt ctccataatg gcactcagat
      601 cgagaatatt cccggtccga accaccaagg gacgatcggg agcgatggtc atggagagga
      661 ccggatcgtt ctttttgcgg aaatcaagaa tctcgctatt gctgcggaag gtcacgagac
      721 gcagcgcagg aatcccgatc cgcccctgga cgttctgctg gttcgagagc gccaggatgc
      781 gaagccctcc attggttccg gcctctgcca cgagagggac agcgagattg ctgcaggaga
      841 agacccctgc cgccagatcc agcacggcgc ccgcacccgg ccgggcgaga ataatccccc
      901 gtctcagatc ccacaagctc cggccggtcc tgcgagggtt ccgcgccacc tgctgatcgg
      961 tccgggcctg acgattggtc gatccgtccg ggtcctgatt ggtcccggca tgcatcaagg
     1021 gagccgagtc cttccccgtc cgttgtgaaa cggcattggt gatctcggag gcaatcttca
     1081 tattggtcag cacgagcgct gtggaagaaa cactccgcgc cgaactcgcc gactgactgg
     1141 ccggaaaatg ttccccgtga agagccggag aggctgcgcg cgacgatgac gcccccaccg
     1201 tcttcccgcg gggtgccgac gaagcccggg tatccgtgtt ggtccggatg gcagggcgtt
     1261 cgttcgtggg acggggcgga ttctcccagg ccgccgaacg gaggacgagg tgagccggac
     1321 cccgcaggcg gagcatcaaa ccgtcccggc cgagcatgtc gatccatgac catgtgtcat
     1381 tgataacgat gtgcgtcccg ctggggatcg aatccgcatc gtcggcccgc ttcccccaac
     1441 tgacctgtcc cttgttggtc accgcacggc cacttgtcga aagccagaca tcgcccacga
     1501 ccttgatgac attggccccg ttggccccgg tggacagggt gaagaccgag gcattgttct
     1561 gcgaggccca gtctgtttcc ttcttggcct gggccgccgt cccgaccacc gccatcgtgg
     1621 aaccacgcca ggagatgatg tccttcccgg tatcataggt cacaaagtcg gcacg
//
LOCUS       DAOSHE010000460         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_74239_length_1674_cov_3.004941, whole genome shotgun sequence.
ACCESSION   DAOSHE010000460 DAOSHE010000000
VERSION     DAOSHE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74239_length_1674_cov_3.004941"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1674)
                     /locus_tag="PLD82_10055"
     CDS             complement(<1..>1674)
                     /locus_tag="PLD82_10055"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG65765.1"
                     /translation="ESLAWAHHGSLARELRLVVEERMKAGQLKAIVSTNTLELGIDIG
                     QLDQVLLVQTPRQVSSALQRIGRSGHGVGETSRAILFPLPGIDLAEAACMAHAVHNRD
                     IEEIRIPESPLDILSQILLSMVVSRDWTADGLYTAILACWPYRNLPRRHFDLVLDLLA
                     GRYAITKVSELSPRISIDPLDGRLKAKPGAARLLYLSGGTIPDRGLFDLRVDGSNAKI
                     GELDEEFVWERSPGDTFALGSQVWRIQSISENAVSVRQVHEPPGIIPFWKQENLPRDW
                     HFSERLGIFMNLMDTTPSNQQEALLMQDYAMRPEAARELRDLVARQKESTGCALPHRS
                     HIVIEHSRESTGPTGSKSVLLHTLWGSRINQPFALALSAAWKRLHGWRLETFVNNDAI
                     ALLVPHEVEATFFSLVTPENLESLLRESLESSGFFGALFRENAGRALLLPKGSFNKRI
                     PLWMTRLRSKKLLDAVSNKPDFPLMLETWRSCLADEFDLPALHQLLDEIGAGTIRISE
                     CHTTTPSPFAEGILWRLTNRFMYEDDTPEQDKPSSLTAELYRQLVNTPH"
BASE COUNT          326 a          444 c          559 g          345 t
ORIGIN      
        1 ggtgtggtgt gttgaccaat tgccggtaga gttccgcggt cagggacgaa ggcttgtcct
       61 gctcgggtgt gtcatcctcg tacatgaagc ggttggtcag gcgccaaaga atcccttctg
      121 cgaatggaga aggtgtggtt gtgtggcact cgctgatacg gatggtgccg gcaccgattt
      181 cgtccaaaag ctggtggagt gcgggcagat cgaactcgtc ggccagacac gagcgccagg
      241 tctcgagcat caggggaaag tcgggtttgt tgctgactgc atccaggagt tttttcgagc
      301 ggagccgggt catccaaagc ggaatccgtt tattgaagga tcccttgggg aggaggagtg
      361 cccgccccgc gttttccctg aagagggcgc caaaaaaacc ggaggactcg agggattcac
      421 gaagcaggct ctccagattt tcgggagtga ccagggagaa aaacgtggct tcgacctcgt
      481 gcggtacgag gagggcgatc gcgtcgttgt tgacaaaggt ttccagacgc cacccgtgca
      541 atcgtttcca ggcggcggag agggccaggg caaagggctg gttgatccgg ctgccccaga
      601 gggtgtgcaa aagaacgctc ttggatccgg tcgggcctgt gctttcgcgg gagtgttcga
      661 tgacaatatg gctacggtgc gggagtgcgc agcctgtaga ctccttttgc cgtgcgacaa
      721 gatcacgcag ttcccgggcc gcttcggggc gcatggcata gtcctgcatg aggagtgctt
      781 cctgttggtt ggagggtgtc gtgtccatca gattcatgaa gatgccgagt ctctccgaaa
      841 aatgccagtc ccgcgggaga ttctcctgtt tccagaaagg gatgatcccc ggcggttcgt
      901 gcacctgtcg gaccgagacg gcattctcgc tgatcgactg gatgcgccag acctgtgacc
      961 cgagggcaaa ggtatcaccc ggactccgct cccagacaaa ttcttcatcg agttcaccga
     1021 tcttcgcgtt gcttccgtcc acccggagat caaagagccc ccggtcgggg atcgttcctc
     1081 cggagagata gagcaggcgg gccgcaccgg gtttggcctt cagccggcca tcgagcggat
     1141 cgatgctgat ccggggcgag agttcggaca cctttgtgat tgcataccgt cccgcaagga
     1201 ggtcgaggac cagatcaaaa tgcctgcgtg gcaggttgcg gtacggccag caggcgagga
     1261 tggcggtgta gagtccgtcc gccgtccagt cacgcgaaac gaccatcgaa aggaggatct
     1321 gtgacaggat gtcgagcggc gactcgggaa tccggatttc ttcgatatca cgattgtgta
     1381 cggcatgggc catgcatgcg gcctcggcca ggtcgatccc ggggagtggg aagagaatcg
     1441 cccggctggt ctctccgact ccgtgtccgg agcgtccgat gcgttgcagg gccgatgaga
     1501 cctggcgcgg tgtctgtacg aggagtacct gatcaagctg gccgatatcg atacccagct
     1561 caagggtatt tgtcgagacg atcgccttga gttgaccggc tttcatccgt tcctcgacaa
     1621 ccagacgcag ttcccgggcg agggatccat ggtgggccca ggccagggat tcct
//
LOCUS       DAOSHE010000461         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_74904_length_1665_cov_2.003106, whole genome shotgun sequence.
ACCESSION   DAOSHE010000461 DAOSHE010000000
VERSION     DAOSHE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74904_length_1665_cov_2.003106"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLD82_10060"
     CDS             <1..680
                     /locus_tag="PLD82_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65766.1"
                     /translation="NSVYGLWLAVPYVLHLVFGTEAKAYMGGQRRQVLLAAVVLPCVV
                     LAGLFAATFVYRDASDRTAMVHPLSSSRLKGILTTGSRARVWNDLMSGLDAYELESRP
                     LLAYPGIPMLVYATGSVPYLGISWPSLLPVDGQEKAFDWARKKYPEYPLVVKALVPTT
                     GRCWPDPEPGVKTVSRGEDRRSRLDALTRAMLQAGKYTKVWSNSAFEIWEPDGYEHKR
                     TGQSVFR"
     gene            789..>1665
                     /locus_tag="PLD82_10065"
     CDS             789..>1665
                     /locus_tag="PLD82_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG65767.1"
                     /translation="MTRPGAYLAALALLSVSLSATEPSRLSLEMALSGVARNNPGLKA
                     AEARVEKARLQVEKAEYLGFLTRFDVNIATGAIPGSDTESYFQNFGPFLKSEIGIVQP
                     IFTFGKISGTERLARFGLDHEREKFADTRDRAALEVIKLYRGLCTAMRAELVARDMLR
                     DFAKLTNQVEQELVKTNASIDDLDKLDVTIKSWQVYQAYTAALEDRTNLMRAMNIALG
                     QPESAEIDVRDEPLPEVALHVTNYEYLVKRVSERHPAMRAMRAALAAADARVAVEKAN
                     NYPDIYLGGGMRFNWA"
BASE COUNT          338 a          430 c          511 g          386 t
ORIGIN      
        1 ggaattcggt gtatggtttg tggctcgctg ttccgtacgt tttgcatctt gttttcggga
       61 cggaggcaaa ggcgtacatg ggcgggcaaa ggagacaagt cctgcttgcg gcggttgtgc
      121 tgccctgtgt ggttcttgcc ggattatttg cggcaacctt tgtctatcgg gatgcctccg
      181 atcgaaccgc aatggttcac ccgctctcct cgtcgcggtt gaaggggatt ttgaccacgg
      241 gatcccgtgc aagggtctgg aacgatctga tgtcggggct tgatgcatac gagcttgaga
      301 gccgtcctct tttggcgtat ccgggaattc ccatgcttgt gtatgcaaca ggaagtgtgc
      361 cctacctcgg gatctcctgg ccatcattgc ttcctgttga cggccaggaa aaggcctttg
      421 attgggctcg gaagaagtat ccggagtatc cactcgttgt gaaagctctt gttccaacaa
      481 ccggacgatg ctggccggac cctgaaccgg gtgtcaagac tgtgtcgaga ggggaggaca
      541 ggagaagcag actggatgcg ttgactcggg caatgctgca ggccggaaag tacacaaaag
      601 tctggagcaa tagtgctttt gaaatctggg agccggatgg gtacgagcat aaacggactg
      661 gccagtccgt ttttcgttga ctttgggatt ctccgactgt aggttttgcc cgtgtccggg
      721 gaactgacgg catcgggatg tgctgatgac tgttccccat aaaaaaccag tccgcagagc
      781 aggtactcat gacccgtcct ggcgcatacc tagcagccct tgcactcctt tctgtttcac
      841 tctccgcaac cgaaccgtcg cggctctccc tggagatggc gctttccggg gtggcccgga
      901 acaacccggg gctcaaggcg gctgaagcac gcgtggagaa ggcccggctt caggtcgaga
      961 aggctgagta tctcgggttt ttgacgcgct ttgacgtcaa tattgcgacc ggtgcgattc
     1021 cagggtctga taccgagagc tatttccaga atttcgggcc ctttctcaag agcgagatcg
     1081 ggattgtgca accgatcttc accttcggca agatctccgg aaccgagcgt ttggcgcgtt
     1141 tcggtttgga tcacgaacgc gaaaaattcg ctgatacccg ggatcgggcg gcccttgagg
     1201 tcatcaagct ctatcgaggg ctctgtaccg ccatgcgtgc ggagttggtc gcgcgcgaca
     1261 tgttgcggga ctttgccaaa ctgaccaatc aggtcgagca ggaactggtc aagaccaatg
     1321 cctccattga cgatcttgac aaactagatg tgaccatcaa gtcctggcag gtctatcagg
     1381 cctataccgc ggcgttggaa gatcgcacaa accttatgcg ggccatgaat atcgcgctcg
     1441 gacaacccga gtcggccgag atcgatgttc gggatgagcc gctcccggag gtagcacttc
     1501 acgttacaaa ttatgaatat ctggtaaagc gggtgtctga gcgccatccg gccatgaggg
     1561 ccatgcgcgc cgccctggcg gccgcggatg ccagggtggc tgtcgaaaaa gcgaacaact
     1621 atccggacat ttatctgggt ggcgggatgc gtttcaactg ggcgg
//
LOCUS       DAOSHE010000462         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_75278_length_1659_cov_4.065461, whole genome shotgun sequence.
ACCESSION   DAOSHE010000462 DAOSHE010000000
VERSION     DAOSHE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75278_length_1659_cov_4.065461"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLD82_10070"
     CDS             <1..365
                     /locus_tag="PLD82_10070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG65768.1"
                     /translation="ELENKKSEFEPAWTDATNAIAKWSPKRLYQAAGYRYTKGLKSRP
                     AVRGSLAMANSGPDSNGSQFFINVADTTWLNGKHTVFGGIVAGQDVADAISKVERNDN
                     DKPKTDIRINAIVLKTAK"
     gene            473..1096
                     /locus_tag="PLD82_10075"
     CDS             473..1096
                     /locus_tag="PLD82_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOG65769.1"
                     /translation="MDASSLPCPQCGAPLEITNRYAKIVVCQYCGAHSALETGNARIL
                     GQFPKLADVPTWLFTGAEGTIQGKRFRAAGRMRYQHKSGFWDEWFLLMEDGTPAWLTE
                     DEGEFSLFEGRKIKTEIPEPDSIKVGTKITINDLVISVMEKGEAAVAGAEGELGFWTA
                     PGTRIRYYEGVAAGKLAALEVSEDEIELFTGDEVMRKEIVIDNRSQG"
     gene            1124..>1659
                     /locus_tag="PLD82_10080"
     CDS             1124..>1659
                     /locus_tag="PLD82_10080"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOG65770.1"
                     /translation="MQCPQCGQEVCEGAGVCPSCSAPIPQGGGQPAVSPGVSGLACPA
                     CGGTRFTRGSKTIEGEEEDDDDVVVSLARCSSCGKVFDKDTPEYWRFFADRFVPGKNG
                     LPLAIGMKGTIGEDKFEILGRIRYQEDELYDDSWWEEWYLLSDSGERGFLVVEDGHFE
                     FYTKTRPVEELTFEEGVVR"
BASE COUNT          369 a          504 c          495 g          291 t
ORIGIN      
        1 aagaactcga aaacaaaaaa agcgagttcg aaccggcctg gacggatgcc accaatgcca
       61 tcgccaagtg gtcccccaag cgcctctatc aggcagccgg ctaccgctac accaaaggac
      121 tcaagagccg tcccgccgtg cgtggctccc tggccatggc caactcgggt cccgactcca
      181 acggcagcca gttcttcatc aatgttgccg acacgacctg gctgaatggc aagcacaccg
      241 tcttcggcgg catcgtggcc ggacaggacg tcgccgacgc gatctccaag gtcgagcgca
      301 acgacaacga caagccgaaa accgatatcc ggatcaatgc catcgtcctc aaaacggcaa
      361 aatgattgcg ggctgacaaa aaaagtcctc gcgtttcttg cgcaaagcga ccggccggga
      421 tatactcaag gccggttgct ttttttattc ccgaggcgag gagtttccga gtatggatgc
      481 cagttccctg ccctgccccc agtgtggcgc acccctggag atcaccaacc gctacgcaaa
      541 gatcgtcgtc tgccaatact gcggcgcaca cagcgccctc gagaccggca acgccaggat
      601 tctgggacaa tttcccaaac tggccgatgt ccccacctgg ctttttacgg gtgccgaagg
      661 caccatccag gggaaacggt tccgggcagc cggccggatg cgctaccagc acaagagcgg
      721 attttgggac gagtggttcc tcctcatgga ggacggcacc ccggcctggc tgaccgagga
      781 tgagggcgag ttctccctct tcgagggacg caagatcaag accgagatcc ccgaaccgga
      841 ctcgatcaag gtcggaacaa agatcaccat caacgatctc gtgatctcgg tcatggaaaa
      901 gggcgaggcc gccgtcgccg gggccgaagg cgagctgggc ttctggaccg ctcccggaac
      961 ccggatccgc tattatgagg gcgttgctgc cgggaaactg gccgccctcg aagtgagcga
     1021 ggacgagatc gaactcttta ccggcgacga agtcatgcgg aaagaaatcg tgattgacaa
     1081 ccggtcacag ggctgaacac ccaaccacac aaggaggacc gcaatgcaat gtccacaatg
     1141 cggacaggaa gtctgcgagg gtgcgggcgt ctgcccgtcc tgttcggccc cgatccccca
     1201 gggtggcggt caaccggccg tttcccccgg cgtgagcggg ctggcctgtc ctgcctgcgg
     1261 gggaacgcgt tttacacggg gaagcaagac cattgaaggc gaggaagagg acgacgacga
     1321 tgtcgtggtc tccctggccc gctgttcctc ctgcggcaag gtctttgaca aggatacccc
     1381 cgagtactgg cgattttttg ccgatcgttt tgtccccggg aaaaacgggc tcccgctcgc
     1441 catcggcatg aagggcacta tcggcgagga caagttcgag attctcggcc ggatccgcta
     1501 tcaggaagac gaactctacg acgattcgtg gtgggaggag tggtaccttc tctcagactc
     1561 cggcgagcgc ggcttcctcg ttgtcgagga cggacacttc gaattctaca ccaagacacg
     1621 tccggtggaa gaattgactt tcgaggaagg tgtggtccg
//
LOCUS       DAOSHE010000463         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_75502_length_1656_cov_3.435353, whole genome shotgun sequence.
ACCESSION   DAOSHE010000463 DAOSHE010000000
VERSION     DAOSHE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75502_length_1656_cov_3.435353"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLD82_10085"
     CDS             <1..190
                     /locus_tag="PLD82_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65771.1"
                     /translation="VTHDADFAASIADSVICLCQGRIIHQGSALDAANPEILKEVFGT
                     DFLRTTAGRILQDYGHPS"
     gene            complement(207..770)
                     /locus_tag="PLD82_10090"
     CDS             complement(207..770)
                     /locus_tag="PLD82_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65772.1"
                     /translation="MQPTLATEDGFDTRDLLQRSEVSIWLDGYDDIFSDFDPRPYTQR
                     AISDDFIAAVRKAIQVKKTGGFELTLLMPKASRDEKTEALIRKRIQNFFQGQFSHHQR
                     EIRKMRREGLILSISSIGVLFTAASLPQMVLDEYLKNLLQVILTPGGWFMIFNGLDSI
                     FYKPRHKKTESDYCRKLARSRIVFVDY"
     gene            921..>1656
                     /locus_tag="PLD82_10095"
     CDS             921..>1656
                     /locus_tag="PLD82_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG65773.1"
                     /translation="MRQYFEGDGYVTPAGRKRAFGDRFFLGTRLWFYLKYIGVLSESR
                     GFVKRNDYGNDNWAWTSWEILDIVEQCGGRVEISGLEHLRSVPKPFVIVGNHMSTLET
                     HILPVMVLPMADMTFVVKKSLTTHFYFGPIMRACHPIAVSRTNPREDLVTVMQEGQRI
                     LGEGRSLAIFPQSTRSRIFDPAQFNSLGAKLAEKAGVPIVPLALKTDFWDNGRLCRDI
                     GPIRRERTVHLEVGQPILPGTCPAREA"
BASE COUNT          343 a          468 c          477 g          368 t
ORIGIN      
        1 cgtgacccat gatgcggatt tcgccgcatc gattgccgat tccgtcatct gtctgtgcca
       61 gggccggatc atccatcagg gttcagccct cgatgcggcc aacccggaga tcctcaagga
      121 ggtcttcgga accgattttc tccggacaac agcgggacgg attcttcagg actatgggca
      181 cccgtcctga cagccagttg taccgatcag tagtcgacaa aaacaatccg gctccgggcg
      241 agttttcggc aatagtctga ttccgttttc ttgtgcctgg gtttgtagaa gatggagtcc
      301 agtccgttga agatcatgaa ccacccgccg ggtgtcagga tgacctggag gaggtttttg
      361 aggtattcat cgagaaccat ctgcggcagg gatgcagcgg taaacaggac cccaatgctc
      421 gagatcgaga ggataaggcc ttccctgcgc atcttccgga tctcgcgctg gtgatgcgaa
      481 aactgtccct ggaaaaaatt ctggatgcgt ttccggatca aggcctcagt cttttcatcc
      541 cgcgaagcct taggcatcaa gagcgtcagt tcgaacccgc cggtcttctt gacctggatg
      601 gctttgcgga ctgccgcgat aaagtcgtcc gagatggctc gctgggtata gggtctggga
      661 tcaaagtcgg aaaaaatgtc atcatacccg tccagccaga tgctgacctc tgagcgctgc
      721 aggagatctc tggtatcgaa tccgtcttcg gtggcgagtg tcggttgcat tccgtaaaca
      781 tactcatcca ggaccgtgtt ctcctgtata acgggcggac acgggatgaa ccctacatca
      841 aggcagtgga cgcccggaac gctgtttgat acagtagatt gtatcaagag tttgttggca
      901 gacggaggaa tcctgtggaa atgcggcagt attttgaagg cgacggatac gtgactccgg
      961 ccggccgaaa gcgcgccttc ggagacagat tcttcctcgg gacccggctc tggttctacc
     1021 tcaagtatat cggggtcctc tccgagagcc ggggttttgt caaacggaat gattacggga
     1081 acgacaactg ggcatggacc tcgtgggaga tccttgacat cgtcgagcag tgcggtggcc
     1141 gggtcgagat cagcggcctg gagcatctgc gctcagtccc caagcctttt gtgatcgtcg
     1201 gtaatcatat gagcaccctc gaaacccata tcctgcctgt catggttctt ccgatggcgg
     1261 atatgacctt tgtcgtcaaa aagagtctga ccacacattt ttacttcgga ccgatcatgc
     1321 gggcctgcca cccgattgcc gtttcacgca ccaatccacg ggaggatctc gtgacggtca
     1381 tgcaggaggg acagcgcatt ctgggcgagg gccgctcact ggccatcttc ccgcagagta
     1441 cgcgcagtcg catcttcgat cctgcccagt tcaactcctt gggagccaaa ctcgccgaaa
     1501 aggccggcgt ccccatcgtg cctctcgcgc tcaagacgga cttttgggac aacggccgac
     1561 tctgccgcga tatcggcccg atccggcgcg agcgcaccgt ccatctcgag gtgggccagc
     1621 ccatcctgcc agggacgtgt ccggcccgtg aggccc
//
LOCUS       DAOSHE010000464         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_75572_length_1655_cov_4.290000, whole genome shotgun sequence.
ACCESSION   DAOSHE010000464 DAOSHE010000000
VERSION     DAOSHE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75572_length_1655_cov_4.290000"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..995)
                     /locus_tag="PLD82_10100"
     CDS             complement(183..995)
                     /locus_tag="PLD82_10100"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOG65774.1"
                     /translation="MNTQDFLELMGKAKIYDLTQRLSVHTPPWPSYMPLQVQYFKRIA
                     GAHMGQGANGQIIKTSNHVGTHIDGEIHFYAAGRSIGDTPLDFWYGPGVVADISKEVE
                     DYSLYTPEMIMKKVEVKKGDILIINTGYNRYAWDQPGSDEIRYFCKHPGPDPSFHKWA
                     LEMNIKWIGVDCGSADHPMNTIIRNWHPKLFTEAEEKMKKEYGKAWDELFPPEEYYQV
                     MHLKLFPRGLVHAENIGGQINEVSNKRVWVGLFPLRGIELESSLCRLVAIEP"
     gene            complement(1118..>1655)
                     /locus_tag="PLD82_10105"
     CDS             complement(1118..>1655)
                     /locus_tag="PLD82_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOG65775.1"
                     /translation="CTLDAARGIKGSSLLVVMARNGTDFGIQLSGTDRQWFTGPALVP
                     DALYFPGFTKEDANPDIGDSAITETFGLGGFAIAAAPAIVQFVGGTARDAINYTMTMY
                     EIAFGENNVYQIPALNFRGTPTGIDVRSVIAKAITPFIDTGVAHKKPGVGQVGAGVVQ
                     APIEPFIKAFEGLAKSLS"
BASE COUNT          342 a          435 c          489 g          389 t
ORIGIN      
        1 catttccact acgtcgctga gaatggcctg ggcactgtac atggttgacc tccgtttgcg
       61 tggataaaga ctggagaaaa tgatgacggt gaatcagggc aagagatgat ggaacagatg
      121 ctgtgctgct gaatgacgta cgcccggcag gtgccgggcg tacgctgaaa tgcacagccg
      181 tctcagggct cgatggcgac cagacggcag agagaggatt cgagttcgat gccgcgcaga
      241 gggaagagac cgacccagac gcgcttgttg ctcacttcgt tgatctgtcc accgatattc
      301 tcggcgtgga caagaccacg ggggaagagc ttgaggtgca tgacctggta atactcttcg
      361 ggcgggaaga gttcatccca tgccttgccg tattcttttt tcatcttctc ttcggcttcg
      421 gtaaagagct tgggatgcca gttgcggatg atcgtattca tcgggtggtc ggcactgcca
      481 cagtccacgc cgatccactt gatgttcatc tccagtgccc acttgtggaa ggacggatcc
      541 ggacccgggt gtttgcagaa atagcggatt tcatccgaac cgggctgatc ccaggcatag
      601 cggttgtatc ccgtgttgat gatcaggatg tcgccttttt tgacttcaac ctttttcatg
      661 atcatctcgg gagtgtagag gctgtaatcc tcgacttctt tcgagatgtc ggccacgaca
      721 ccgggcccgt accagaaatc gagcggtgta tcaccgatgg accggcctgc cgcataaaaa
      781 tggatctcgc cgtcaatatg tgtcccgacg tggttgcttg tcttgatgat ctggccgttt
      841 gcaccctggc ccatgtgggc accggcaatg cgtttgaagt actggacctg gaggggcata
      901 tagctgggcc agggcggggt gtgaacgctc aaccgctggg tcagatcata gatcttggcc
      961 ttgcccatca attcgagaaa atcctgggta ttcattcatc tctccttgtt tggtttctgc
     1021 ttctttcgta aaaaatagtc atttacagat gcaagtgact ttttgggaac aacctcagtc
     1081 ctgaccggga gcgagatgat cccggtcagg tttggactca cgagagactt tttgccaagc
     1141 cctcaaaggc tttgatgaac ggctcgatcg gtgcctggac cacaccggca ccaacctggc
     1201 caacaccggg ctttttatgg gctacaccgg tatcgatgaa gggcgtgatg gccttggcga
     1261 tgacgctgcg gacatcaatt ccggtcgggg tcccacggaa attgagggct ggaatctggt
     1321 agacgttgtt ttcaccaaac gcgatctcgt acatggtcat cgtgtagttg atggcgtccc
     1381 gggccgtccc tcccacgaac tggacgatgg ccggagccgc cgcgatggca aatccgccca
     1441 acccgaaagt ctcggtgatg gcgctgtccc cgatatccgg gttggcgtct tccttggtga
     1501 aaccggggaa gtagagcgca tccggaacga gagctgggcc ggtaaaccac tggcggtccg
     1561 taccggaaag ctggatcccg aagtctgtcc cgttgcgggc catgaccacc agcaggctgc
     1621 ttcccttgat gccacgggcg gcatcaagcg tgcat
//
LOCUS       DAOSHE010000465         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_75799_length_1652_cov_3.176581, whole genome shotgun sequence.
ACCESSION   DAOSHE010000465 DAOSHE010000000
VERSION     DAOSHE010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75799_length_1652_cov_3.176581"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLD82_10110"
     CDS             <1..247
                     /locus_tag="PLD82_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG65776.1"
                     /translation="LPIITLVGSLIPALVSGSVIVERIFSLPGIGQLFFESVLYRDYT
                     TIMGLSVLSAILTLFGILLADILYAIADPRISYGRKT"
     gene            244..1218
                     /locus_tag="PLD82_10115"
     CDS             244..1218
                     /locus_tag="PLD82_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65777.1"
                     /translation="MSPAMQRFFANRTGKIGLGIVGFFVLLAILAPVLANDAPIVLVR
                     NGEWFFPAIFDHDEFANVDWQVWLEKEPYDSALMPPVPFSPVRVRLDEALLPPGEKGH
                     LLGTDNLGYDILARIIHGSRIALSVGIVAMGIAALIGLVLGSLAGYFGGSVDILISRL
                     IEIVICFPLLFLILTVLAFLPPSIYNIMIVIGITSWTGIARLVRGEILKVREMDYVKA
                     ARAAGYPDRRIISHHVLPNALAPVLVSISFGIAGSILTETALSFLGFGVQPPDPSWGQ
                     ILSLAREYPSRIWFTIFPGLAIFLTVTGYNMIGEALRDALDPRLARRE"
     gene            1366..>1652
                     /locus_tag="PLD82_10120"
     CDS             1366..>1652
                     /locus_tag="PLD82_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65778.1"
                     /translation="MTEKVPLKLIWTCTAADEREPAWLVTAEADEPNGDVTDEVATTL
                     LTDEVEAVLLTDEVEAVLLTDEADPNRDDVEEALLDEGMYTNCGAVGAVLSS"
BASE COUNT          317 a          479 c          467 g          389 t
ORIGIN      
        1 ccttccgatc atcaccctgg tcgggagtct gattccggcc ctcgtctcgg ggagcgtgat
       61 tgtcgagcgg attttcagcc tgcccgggat tggtcaactc ttctttgaaa gtgtcctcta
      121 ccgggattac acgaccatca tggggctctc ggtcctctcc gccatcctga cactcttcgg
      181 aatcctcctg gccgacattc tctatgcaat cgccgatccc cggatcagtt acgggaggaa
      241 gacatgagcc cggcgatgca acgatttttt gccaaccgga ctggaaaaat cggactgggg
      301 attgtcggat tctttgttct cctggcaatt ctggctccgg tcctggcgaa tgacgccccg
      361 atcgtgcttg tccggaacgg ggaatggttt ttcccggcca tttttgacca cgacgagttc
      421 gcgaatgtcg attggcaagt ctggctcgaa aaggagccgt acgactcggc cctgatgccc
      481 cccgtgccgt tctccccggt ccgagtccgc ctggatgaag ccctcctccc tcccggggaa
      541 aaggggcacc ttttgggaac cgacaatctc ggctatgaca tcctggcccg gatcatccac
      601 gggagcagga ttgccctctc cgtgggaatc gtcgccatgg ggatcgcggc cctgatcgga
      661 ctggtcctcg gcagtctggc cgggtatttc ggcgggagcg tcgacatttt gatctcgcgc
      721 ctgatcgaaa tcgtgatctg ttttcccctc ctttttttga tcctcacggt tctggccttc
      781 ctgcctccat cgatctacaa tatcatgatt gtgatcggga tcaccagctg gaccgggatt
      841 gcccgcttgg tccgtggcga gatcctcaag gtccgcgaga tggattacgt caaggctgcc
      901 cgggctgccg gttatccgga ccggaggatc atcagccatc atgtcctccc gaacgcactg
      961 gccccggtct tagtctcgat ctccttcggg attgccggtt cgattctgac ggaaacggca
     1021 ctctccttcc tcggattcgg ggttcaaccg ccggatccat cgtggggaca gatcctctct
     1081 cttgccaggg aatacccctc ccggatctgg tttaccatct tccccggtct ggcaattttc
     1141 ttgaccgtga cgggatacaa catgatcgga gaagcgctcc gggatgcact cgaccctcgc
     1201 ctggcccgca gggaatgaca ccgcggtcac gaacctgccg gatcatacac actctgtctc
     1261 gttgaaaaaa ggctgtccct gataccagga acagccttct gtagtgtaca gcccttcgtc
     1321 ctatggggac acacttttca ttttggcgtt acatcggcat agcgcttgac tgagaaggtt
     1381 ccgctgaagc tcatttggac ttgtacagca gccgatgaga gagaaccagc ttggctcgtt
     1441 acagcagaag cagacgagcc aaacggtgac gtgacggacg aggtggcaac aacactgctg
     1501 acggatgaag tcgaggcagt actgttgacg gatgaagttg aggcagtact gttgacggat
     1561 gaggctgatc cgaacaggga tgacgttgag gaagcactgc ttgacgaggg aatgtacaca
     1621 aactgcggtg cggtcggagc ggtactgtca tc
//
LOCUS       DAOSHE010000466         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_76123_length_1647_cov_4.621859, whole genome shotgun sequence.
ACCESSION   DAOSHE010000466 DAOSHE010000000
VERSION     DAOSHE010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76123_length_1647_cov_4.621859"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..449
                     /locus_tag="PLD82_10125"
     CDS             99..449
                     /locus_tag="PLD82_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG65779.1"
                     /translation="MQNQTKKYEHSYSKKNTLKWLFRFDVHYRCKRLTELFRKHRIDI
                     ENKRVMDFGFGGGQLLKIFPKTCSLTGADVSRSGVERARQDPDFRQYKAANFVQVKED
                     GVSSYPKKALLTSL"
     gene            446..856
                     /locus_tag="PLD82_10130"
     CDS             446..856
                     /locus_tag="PLD82_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65780.1"
                     /translation="MTSHTLEHVLDDLATLRAMYTSLKKGGILAVFVPVEEPDYIQFH
                     VRNYSVQGIGNLVARAGFEVIHHEGSMYVNGHLWKLITIPSRRHWPVIGQIVNGIRVV
                     SLSLLPYPILKIFDRFLYAIGVGARQACVIARKP"
     gene            complement(982..>1647)
                     /locus_tag="PLD82_10135"
     CDS             complement(982..>1647)
                     /locus_tag="PLD82_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOG65781.1"
                     /translation="NVRSMQNGETVEIAATDPGFAIDIKAWCERTGNQLVNLSSAGGR
                     FTARVRKGQMPTAAPQACTRGPNDKTIVLFSGDFDKAMAAFIIGTTAASMGGKVTIFF
                     TFWGLNLLRQHKKVRVKKTLIERMFGMMMPRGSRRFKLSKMHMAGMGTGMIKRIMRKK
                     HVPSLEELINAARESGIRLVACQMTMDLMGIKPEELIEGVEKGGAASYIGAADTANMN
                     LFI"
BASE COUNT          349 a          529 c          400 g          369 t
ORIGIN      
        1 cctgaaatac gccgatccgg cacaataccg acgcgtgtac cttgaaattt ccgttttctg
       61 cgatatcatt ccccctacac tctaaaagca ggaaaccgat gcagaaccag acaaagaaat
      121 acgaacactc ctacagcaaa aaaaacaccc tcaaatggct tttccgtttc gatgtccatt
      181 accgatgcaa gcgcctgacc gagcttttcc gcaaacaccg gatcgatatc gaaaacaagc
      241 gagtcatgga tttcggtttc ggtggcggac aactcctcaa gatcttcccc aagacatgca
      301 gcctgaccgg agccgatgtc tcccgatcgg gagtcgaacg cgccaggcag gatcccgatt
      361 tccggcagta caaggccgca aattttgtcc aggttaagga ggacggtgtc tcgagctacc
      421 cgaaaaaggc ccttttgaca tcattgtgac ttcacatacg ctcgaacacg tcctggatga
      481 tctcgcaacc ctgcgcgcca tgtacacctc cctcaaaaag ggcgggatcc tcgccgtctt
      541 tgttcccgtc gaggaacctg attacatcca gttccatgta cggaactact ccgtccaagg
      601 gatcggaaat ctcgtggccc gggccggatt tgaggtgatc caccatgagg ggagcatgta
      661 tgtcaatggc catctctgga agctgatcac catccccagc agacgtcatt ggccggtcat
      721 cggccagatc gtcaacggga tccgggttgt ctcgctttca ctcctgccct acccgattct
      781 caaaatcttt gaccgtttcc tctacgccat cggagtcggg gcccgccagg cctgcgttat
      841 cgcccggaaa ccctgacggg caggatcgac gtccatcgac tggcacaccg gcctcaccct
      901 tcagcctctg tccatcccga catgcagcga agggccgccc cgacgtttcg gtggcagccc
      961 ttcgtgtatc ccgcccatgc ttcagataaa gagattcata ttggccgtat cggccgcgcc
     1021 gatatagctg gcggccccgc ccttctcgac tccctcgatc aactcttcgg gcttgatccc
     1081 catcagatcc attgtcatct ggcaggccac cagccgaatt cctgactccc gggccgcatt
     1141 gatcagttcc tcaagggatg gaacatgctt tttccgcatg atacgcttga tcatccccgt
     1201 tcccataccg gccatgtgca tcttcgagag cttgaaccgt ctgctgcccc gcggcatcat
     1261 catcccgaac atccgctcga tcagcgtttt tttgactcgc accttcttgt gttggcggag
     1321 aaggttcagc ccccagaatg tgaagaagat ggtcactttt ccacccatcg aggctgccgt
     1381 tgtcccgatg atgaaggcag ccatggcctt gtcaaaatcc ccactgaaga gaacaatcgt
     1441 cttgtcattc ggtccacgcg tgcacgcctg gggggccgcc gtcggcatct gacccttgcg
     1501 gaccctggcc gtaaatcgtc ccccggcgct ggagaggttg acgagctggt ttcccgtcct
     1561 ctcgcaccag gccttgatat cgatcgcgaa cccgggatcc gttgccgcga tctcaacggt
     1621 ctcaccgttt tgcatgctgc gcacatt
//
LOCUS       DAOSHE010000467         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_76733_length_1639_cov_2.506313, whole genome shotgun sequence.
ACCESSION   DAOSHE010000467 DAOSHE010000000
VERSION     DAOSHE010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76733_length_1639_cov_2.506313"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLD82_10140"
     CDS             <1..1201
                     /locus_tag="PLD82_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOG65782.1"
                     /translation="EQAGLAEMLRSLTGSACGRILVDVTGHKKSTAVRLTMAAMRMES
                     VDLAYLDGRGIKDHIPGSAAPRPGTERILFWSGRDRREAREQSVSLVENECITVSSRG
                     GTAFAVRVRKGISHIEAVVPRAPLEPILARIGNPACSRSELLACGHRFHEHLIPPELR
                     AEIGLALSGRDITFVCSDQDAADLPFEFLVHQGSFLGEQTPVRRILPADTPGSGRSRA
                     VTTRRMLILEGAGPGGWYPAATREVRDLAALAQSFGIPVTVMRGASIEEVLLQARQHG
                     ILHFVGHGDTRGVFLADGILEPGVVEGLTGMAGFVFLNACESGSSRMAGAMLAAGTQT
                     CIGARQVIRDGLASQGVIAMYRRLFETADVLAAAMELVTEDAVLPYRIWGSRWLGRED
                     TRGEPSG"
     gene            1251..>1639
                     /locus_tag="PLD82_10145"
     CDS             1251..>1639
                     /locus_tag="PLD82_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65783.1"
                     /translation="MKIRYGVVFGVFLFGVTALSASWMGETWLWVSVRSVAQSTNNSL
                     TVSAELDIKYWGLGRGHGTTYGKEFPGKTFRVHLSCPSQDVARGLQAGQTGWVKHHAS
                     SWLYRDEDGKPRGGSSSRWEWLGDTAW"
BASE COUNT          290 a          434 c          571 g          344 t
ORIGIN      
        1 tgaacaggct ggactggcgg agatgctgcg ttcgttgacg ggttcggcct gcggccggat
       61 tctggtcgat gtgaccggcc acaaaaagag taccgcggtc cgcctgacga tggccgccat
      121 gaggatggag tcggtggacc tggcctatct tgacgggcgc gggatcaagg atcatatccc
      181 gggctccgcg gcaccccgcc ccggcacaga gcggatcctg ttttggagcg ggcgggaccg
      241 gcgcgaggca cgcgagcaga gtgtgtcgct tgttgaaaac gaatgcatta cggtctcatc
      301 ccgtgggggc acggcgtttg ccgtgcgcgt gaggaagggc atctcccata tcgaggccgt
      361 ggtcccacgg gcgccgcttg aaccgattct tgcaaggatc ggcaatcccg catgttcccg
      421 ctccgagctc ctggcctgcg ggcacaggtt tcatgagcac ctgatcccgc ctgagctgcg
      481 cgcggaaatc ggactggccc tttcagggcg tgatatcacc tttgtctgca gcgatcagga
      541 tgcggctgat ctccctttcg agtttttggt gcatcagggt tcttttctcg gggaacagac
      601 tccggtccgc aggattttgc cagcggacac gccgggttcg ggccggtccc gggcggtgac
      661 gacacggcgc atgctgattc tcgagggagc ggggccgggc ggatggtatc cggccgcgac
      721 ccgggaggtc cgggacctgg ccgcgcttgc ccagtccttc ggaataccgg tcacagtcat
      781 gcggggggca tccatcgagg aggtactcct ccaggcacgg caacatggaa ttctccattt
      841 tgtcggacat ggagataccc ggggcgtctt tctcgctgac gggattcttg aaccgggagt
      901 ggtcgagggt ctgacaggga tggcgggatt cgtattcttg aacgcctgcg agtccgggtc
      961 ttcccggatg gccggcgcca tgctggcggc tggaacccag acttgtattg gtgcacggca
     1021 ggtcatccgg gatggtctgg caagccaggg cgttattgcc atgtaccgga gactctttga
     1081 gacggccgat gttcttgcgg cggccatgga actggtcacg gaagacgctg tgttacccta
     1141 caggatctgg gggagtcgct ggcttgggcg cgaggatacg cgtggtgaac catccggctg
     1201 aagcgctgta gaatgcaggg tgaaagcaag tatccttccg gaggcagaca atgaagatca
     1261 gatatggggt tgtgttcggt gtgttccttt ttggtgtgac ggcactctcg gcgtcatgga
     1321 tgggtgagac ctggctgtgg gtgtcggtca gatccgttgc ccagagtacc aacaacagtc
     1381 tgacggtgtc ggccgaactt gacatcaagt actggggttt gggacgaggc catgggacca
     1441 cctacgggaa agagtttccc gggaaaacct ttcgggttca tctctcctgt ccttcgcagg
     1501 atgtggctcg tggcttgcag gctggacaga ctggctgggt caaacatcac gcatcaagct
     1561 ggttgtatcg ggacgaggat ggaaaaccca gaggtggaag cagctcccgc tgggagtggc
     1621 tgggggacac ggcatggat
//
LOCUS       DAOSHE010000468         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_77018_length_1635_cov_2.712658, whole genome shotgun sequence.
ACCESSION   DAOSHE010000468 DAOSHE010000000
VERSION     DAOSHE010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77018_length_1635_cov_2.712658"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..>1635
                     /locus_tag="PLD82_10150"
     CDS             179..>1635
                     /locus_tag="PLD82_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG65784.1"
                     /translation="MIDTTESAFGLAQSLLDAIPEACLLLSYDAPLLANTAFFSASGY
                     PPGTFNNRCPGSLIAPHYRQAFIDAVRQVESGNPLPALILETLHQNGTSTPVLFHLKK
                     FDHPLLSGILVTHADISANGPIGRDFLRTQAQLEAIVRMLPLIAFILDKNLVIVELLA
                     TRRGMQGLTGSEYSGKHLFELFPESIGNHLAELVSQTLADSEEHVTEYELDLIGDHHW
                     FEARARALESHKSPACNTLIIILDITERHKSFHRIRKNEARYRAIVNTLPDAVCRWLP
                     DLKLTFVNDSYCGLFGQRDHLIGHRWTEFLPKNEISTIEDRYAILVKSPQTITYEHPI
                     IDIHGNTRIIVWTETPIFSSDGTLLEYQSIGRDITSQRSMEFRLARHESVLHSIIHSI
                     PNPVFYKDVEGRYMGCNPAYARLLGLQETEIVGQTINNIIPPELGDKLHKSDGLILSG
                     SEEDSFQVDLTGPDGLDHRVLINKAPFQDDHGNLAG"
BASE COUNT          405 a          502 c          343 g          385 t
ORIGIN      
        1 ggtccagcat tcttttctcc atatccgggg gattcttcca cttcaagata caaaattcgc
       61 cacggtccca gcaagacacc ttccggcctg caatgactgc gaatgctaaa gaagaatatt
      121 ttggtccttg tattttctcc tttccggata tacactgcca tccgaaggga gctgcgcgat
      181 gattgatact accgagtctg cttttggtct ggcccaatcc ctcctggatg caatccccga
      241 ggcatgcctt ctcctctcat acgatgcgcc actcctcgcc aacacggctt ttttctcggc
      301 atcgggctat cctcctggaa ccttcaataa tcgctgccct ggatccctga tcgcgcctca
      361 ctatcgccaa gcgttcatcg acgcagtccg ccaggtcgaa tccggcaacc cgcttccagc
      421 cctgatcctc gaaacccttc accagaacgg gaccagcaca cctgttctct ttcatctcaa
      481 gaaattcgat caccctctcc tgtcagggat tcttgtcact catgcagaca tctcagcaaa
      541 tggcccgatc ggccgggatt ttttacgaac ccaggcacaa cttgaagcca tcgttcgcat
      601 gctcccactg attgccttca ttcttgacaa aaatcttgtg attgttgaac tccttgcaac
      661 cagacgaggg atgcaagggc tgacaggttc cgagtattcc gggaagcacc tctttgaatt
      721 gttccccgaa tcaatcggca accatctggc cgaacttgtc tcacagacac tggcagattc
      781 cgaagaacat gtgacggaat acgaactgga cctgatcggc gaccatcact ggtttgaagc
      841 acgggcccgt gccctggaat ctcacaaatc gccggcttgc aacacattga tcatcattct
      901 cgacattacc gagcggcaca agtcattcca caggatccgg aaaaacgagg ccaggtaccg
      961 ggcaatcgtc aacacccttc cggacgctgt ctgccgctgg ctccctgacc tgaaactgac
     1021 ttttgtcaac gacagctatt gtggtctctt tgggcaaaga gaccacctca tcggacatcg
     1081 ctggacagaa tttcttccaa aaaatgaaat ctctaccatc gaggaccgat atgccattct
     1141 tgtcaaatca ccccaaacca tcacctacga acaccccatc attgatattc acggaaacac
     1201 gcgaatcatt gtctggactg aaaccccaat tttttcatct gatggtacct tgctcgaata
     1261 tcaaagcatc ggtcgggaca taacatccca acgcagcatg gagttccgtc tcgcacgcca
     1321 tgagtcagta ctccattcca tcatccactc catccccaat cccgtcttct acaaggacgt
     1381 cgaaggcaga tacatggggt gcaacccggc ctatgcccgc cttcttggtc tccaggaaac
     1441 cgagattgtc ggacaaacga tcaataacat catacctcca gaacttggag acaaactcca
     1501 taaaagtgat ggcctgatcc tttccggcag cgaggaagac agctttcagg ttgacctgac
     1561 aggtcccgat ggtcttgatc atcgagtcct gatcaacaaa gccccgttcc aagatgacca
     1621 tgggaacctg gccgg
//
LOCUS       DAOSHE010000469         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_77392_length_1629_cov_3.463151, whole genome shotgun sequence.
ACCESSION   DAOSHE010000469 DAOSHE010000000
VERSION     DAOSHE010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77392_length_1629_cov_3.463151"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLD82_10155"
     CDS             complement(<1..774)
                     /locus_tag="PLD82_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG65785.1"
                     /translation="METTCCTSADQFLAALTTITDKNGLRYDPETVRRAWEYAALCHR
                     EQKRLSGESYITHPSETALIVASMGIDQTSIIAALLHDTVEDSAEANPETIRTEFGSD
                     VLHIVEGVTKIKRLRAAHGQADRSAENMRKLLLAMAQDIRVIFVKLADRLHNMRTLAW
                     QPPERRLYTARETLDIYAQLANRLGISWIKTELEDLSLKHLDPDAYYALKKSIDDTRR
                     ERDDRINRVMQEIGSELRHQGLTVEIKGRAKHFYSIYQKM"
     gene            complement(778..>1629)
                     /locus_tag="PLD82_10160"
     CDS             complement(778..>1629)
                     /locus_tag="PLD82_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65786.1"
                     /translation="QQRVGLFFLVSRPAPGLQSGMHSSRPHTALLALFFVVLLLPETG
                     HPAETAPIKWKAALTRAWRDHAAGRFPQSRLAIRQACAYGLERNDWRAVYEGFIILQD
                     STTRGETIPAYFQTYLERASCLPGATNSHLACRLFARIFLARNNPDHAIPLAVRSIEL
                     ALDAGLTGDALQAASLLLPENTGAALNHESVQAWIAIARTSLSSRSWSDTLQAAGLMH
                     KADLHGLAREWINRAAAMALANNDAVTVNTSCVLLIAAGYPDEAAVWQQRLRSVSGRP
                     SASNGNG"
BASE COUNT          271 a          430 c          583 g          345 t
ORIGIN      
        1 catcttctgg tagatgctgt aaaaatgctt ggcccgtccc ttgatctcca cggtgaggcc
       61 ctggtggcgg agttcgctac cgatctcctg catgacacgg ttgatgcggt cgtcccgttc
      121 gcgacgggta tcgtcgatgc tttttttgag ggcgtagtag gcatcgggat cgaggtgctt
      181 gagggaaagg tcttcgagtt ccgtcttgat ccagctgata ccgaggcggt tggcgagctg
      241 ggcgtagatg tcgagggtct cccgtgcggt atagagtctg cgctcgggtg gctgccaggc
      301 cagagtccgc atgttgtgga gccggtcagc cagtttgacg aagataaccc ggatatcctg
      361 tgccatcgcc aagaggagtt ttcgcatgtt ttcggcggac cggtcggcct ggccgtgggc
      421 ggcccgcagc cgtttgatct tggtcacgcc ttcgacgatg tgcaggacat cgcttccgaa
      481 ttcggtacgg atggtctccg gattggcttc ggcggaatcc tcgacggtgt catgcaggag
      541 ggctgcgatg atgctggtct ggtcgattcc catggatgcg acgatcaagg ccgtctcgga
      601 gggatgggtg atataactct cgcccgagag ccgtttctgt tcccggtggc aaagggcggc
      661 atactcccag gctctgcgga ccgtttcagg atcgtagcgc aagccgtttt tgtccgtaat
      721 ggttgtgagc gcggccagga actggtcggc actggtgcag caggtggtct ccatcgatca
      781 accgttcccg ttgctggcgg atggtcttcc ggagaccgat ctgagccgct gttgccagac
      841 tgcagcttcg tcgggatagc ctgcagcgat gaggagcacg caggaggtgt tgacagtgac
      901 ggcatcattg ttggcgaggg ccatggccgc cgccctgttg atccattccc gggccagccc
      961 gtggaggtcg gctttgtgca tcaggccggc agcctggagc gtatcgctcc aggagcggga
     1021 actgagactg gtccgggcga tggcgatcca ggcctggacg gattcgtggt tgagggcggc
     1081 cccggtgttt tcggggagaa ggaggctggc cgcctggagc gcgtcacccg tcaggccggc
     1141 atccagggcg agttcgatcg agcggacagc cagagggatt gcgtggtccg ggttgttccg
     1201 ggcgaggaag atccgggcaa agagccggca ggccaggtgc gagtttgtcg cgccgggaag
     1261 acagctggcc cgttcgaggt aggtttgaaa ataggccggg atcgtctcgc cccttgtcgt
     1321 cgagtcctgg aggatgatga agccctcgta gacggcccgc cagtcgttgc gttcgagccc
     1381 gtaggcgcac gcttgacgga tggccaggcg gctttgggga aaacggccag cggcatgatc
     1441 acgccatgcc ctggtgagtg cggctttcca ttttatcggg gcggtttccg ccgggtggcc
     1501 tgtctccgga aggagcagca ccacgaagaa cagagccagg agcgcggtat ggggccgtga
     1561 ggaatgcata ccggattgta gcccgggtgc gggcctgctg acaaggaaaa agaggcccac
     1621 tcgctgttg
//
LOCUS       DAOSHE010000470         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_77939_length_1622_cov_3.622846, whole genome shotgun sequence.
ACCESSION   DAOSHE010000470 DAOSHE010000000
VERSION     DAOSHE010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77939_length_1622_cov_3.622846"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLD82_10165"
     CDS             <1..215
                     /locus_tag="PLD82_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65787.1"
                     /translation="TTSSGVYLNKLGRSVTATEILANNADILPVILWEDKFPERKQLS
                     ACNIAPEILAKISALETILGELTGKN"
     gene            complement(233..1132)
                     /locus_tag="PLD82_10170"
     CDS             complement(233..1132)
                     /locus_tag="PLD82_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG65788.1"
                     /translation="MTEVEITRDFNLTDEQVIRCEMHSFTNLVNSIFYELELLAGELG
                     DPEAFSGEMRVCEQIVSSFRNRDQALESVHQAESFARQILHAVRERIEGSGRTDLGEY
                     RENIERMMEVVEVRTREMLARFRMPGRWVDLDASGIRDGLLQVLDAIAFSSHGRFGFV
                     YDSGGSDSRYGFSLQVEGARDGRVTMPPVFIDCLRDLVANARKYTKPGGTIEATLSQT
                     EEGFSIKVKDTGLGIPEDEIQQVVEFGVRGSNVREIPTRGGGFGLTKVWYATRQYGGR
                     LWITSAPGQGTLVHVRVPRADRT"
     gene            1410..>1622
                     /locus_tag="PLD82_10175"
     CDS             1410..>1622
                     /locus_tag="PLD82_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65789.1"
                     /translation="MARQASPILSMIIGVLGIAFAGYMYVSQSTFIKKSVETEGTVVE
                     IDKTRKRSKDTHHFSYTYRPVISYVAW"
BASE COUNT          389 a          524 c          371 g          338 t
ORIGIN      
        1 tgactacttc aagcggagtc tatctcaata aactcggacg atcggtcacc gccacggaga
       61 tcctggcgaa caatgcggac atcctccctg tcatcctctg ggaggacaaa tttccggaac
      121 ggaaacaact ctcggcctgc aacatcgccc cggaaatcct tgcaaaaatc tccgctctcg
      181 aaacgatcct gggggaactg accggaaaga actgaccctc cgcaccatca tgtcatgttc
      241 gatcggcccg tggcacccgg acatgcacaa gtgtcccctg ccccggagcc gaagtgatcc
      301 agaggcgtcc gccatactgt cgggttgcat accagacctt ggtcaaaccg aatccgccgc
      361 cccgggtcgg gatctcccgg acatttgaac cacgcactcc aaactcgacg acctgctgga
      421 tttcgtcctc agggatgccc agacccgtat ccttgacctt gatggaaaac ccctcttcgg
      481 tctgactgag ggtcgcctcg atcgtcccgc ccggctttgt atactttcgc gcattggcga
      541 caagatcccg gagacaatcg ataaaaaccg gaggcatggt cacccgaccg tcacgggcgc
      601 cttcgacctg cagggaaaag ccgtagcggg agtcgctccc gccggaatca tagacaaacc
      661 cgaaccggcc atgggaactg aaggcaatgg catccaggac ctgcaacagc ccgtcccgga
      721 taccggaagc atccagatca acccatctcc ccggcatcct gaatcgtgcc agcatctccc
      781 tggtccggac ctcgacaact tccatcatcc tctcgatgtt ctccctgtat tccccaagat
      841 ccgtccggcc actgccttcg atccgctcgc gaaccgcatg caggatctgc cgggcaaaag
      901 actcggcctg atggacgctt tccagtgcct gatcccggtt tcgaaagctc gaaacaatct
      961 gctcgcagac ccgcatctcg ccggaaaaag cttccggatc acccaactca cccgccagca
     1021 actcgagttc ataaaaaatg ctgttcacaa gatttgtaaa actgtgcatt tcgcaccgga
     1081 tcacctgttc atccgtgagg ttgaaatccc gcgtaatctc gacttccgtc attcgaccct
     1141 actctctcat ctccctgttt cccaacatac ctcacttccc agtatagccg aagtccctgt
     1201 gaaatgcaaa atcgccctgt gcacgggaaa ctgaaagcca tcaacatttc atcatgcatc
     1261 cgcatccatc ctcccggccg gatggctcac ctgcaatcgt cacatcgggg aatgcatcca
     1321 atccatgaca caggaacagc ttcctgggct tgaaaaattg acccggggtt ctatactggg
     1381 ctcagtgact acagacaagg gggcagtgca tggcacgaca ggcatcacca atactttcca
     1441 tgattattgg cgttctcggg atcgcttttg ccgggtatat gtatgtttcg caatcaacct
     1501 tcatcaaaaa atctgtcgag accgagggta cggttgtcga aatcgacaag acaagaaagc
     1561 gctccaaaga cacccatcac ttctcgtaca cctatcgccc tgtgatcagc tatgttgcct
     1621 gg
//
LOCUS       DAOSHE010000471         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_78448_length_1615_cov_4.510256, whole genome shotgun sequence.
ACCESSION   DAOSHE010000471 DAOSHE010000000
VERSION     DAOSHE010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78448_length_1615_cov_4.510256"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..456)
                     /locus_tag="PLD82_10180"
     CDS             complement(22..456)
                     /locus_tag="PLD82_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65790.1"
                     /translation="MAFTNDIISNINRREPIGIDKNGTPYRVLVVDDSTVMRKIVTQI
                     LRSEAFEIVSEASNGEEGVEMYNEYKPHLVTMDINMPIMDGLTALKKILSIDPAARVV
                     MLTSESEQKMVLEAIQSGAKNYVVKPPDRAILLEKVKASLGK"
     gene            1017..1382
                     /locus_tag="PLD82_10185"
     CDS             1017..1382
                     /locus_tag="PLD82_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOG65791.1"
                     /translation="MCQYVRARSPSPRQATPRYGDAALSALFRSLIGDGSRVSNLEAW
                     IFGGAVPENSGADMHRFAADAIAIAREFLARRGVAIVSEDVGGVVGRKIAFDLLSGQA
                     MVLKTRVLRKRDWAPFVSA"
     gene            1464..>1615
                     /locus_tag="PLD82_10190"
     CDS             1464..>1615
                     /locus_tag="PLD82_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG65792.1"
                     /translation="MDQVQSSAGHATGPIEAKPGKYLTYRLGGEQYGLEIMKVQEIIG
                     LMPMTRV"
BASE COUNT          332 a          396 c          487 g          400 t
ORIGIN      
        1 gggcggtttg ctgtccctgg atcattttcc caggctggct ttgacctttt caagcaagat
       61 cgcgcgatcc ggaggtttga cgacgtagtt ttttgcgccg ctttgaatcg cttcgaggac
      121 catcttctgc tcactctcgc tggtgagcat gacgacacgg gctgcgggat cgatgctgag
      181 gatctttttc agggccgtca atccgtccat gatgggcata ttgatgtcca tggtgaccaa
      241 atggggcttg tattcattgt acatctcgac cccttcctcg ccattggatg cctcgctgac
      301 aatctcgaaa gcctcggagc ggaggatttg cgtgacgatc ttgcgcatga cggtcgaatc
      361 atcgacaaca aggacacggt atggcgtgcc gtttttatct ataccgattg gttcgcgtct
      421 gttgatattc gagatgatat cgtttgtgaa cgccatggtt tcccccaccg tttgttcccc
      481 ggccctgaac cggagcgcag gaatcacttc ttcgtgacaa tagtatattc attgtcctgg
      541 aaaaatcaag atctcattcc aaggaatgca ctgcttggtt ccggttgaag gtccccatag
      601 gagagcgtgc cggcgctcca gcgcaaaatc atggaaacgg tatgcgattc cgggattgtc
      661 atgccagatc cgcacttttg tcatacaact ccgggaatag tgtacgaatt tcttgaaaac
      721 tgccaattcc atggaacgaa tcgggcgtca tcccgcaaga tgcgggttgt ccatcagcca
      781 aagtgtcctt tcatgagcga attgccgata catacagtgt gtgtactttc tgcaggagac
      841 cgacggaagg catgatggaa cgacgacggg atatgagcgg cagatggatc ggtcaaaacg
      901 catacctttt ggcacccggc tgggtgatga tcagttgcca gccccttcgg ttccatgcgg
      961 tccttggtgt ttcggtggct gtctgcattc gtgacgggac ccgtatcagc ggaggcatgt
     1021 gccagtatgt tcgggcccgt tctccgtccc cgcggcaagc aacaccgagg tatggcgatg
     1081 cggcgctctc agctcttttt cggagtctga tcggggatgg atcacgtgtc tccaaccttg
     1141 aggcgtggat tttcggcggt gcggttccgg agaactctgg ggcggatatg catcgctttg
     1201 cagccgacgc gattgcgatt gcgagggagt ttctcgccag gcgcggagtt gcgattgtca
     1261 gtgaggacgt tggcggtgtt gtcggccgga agattgcatt tgatctcctg agcgggcagg
     1321 ccatggtact aaaaacacgg gttttacgga agcgggattg ggcgccgttt gtctcggcct
     1381 gatggactgg tccgatccca tattcttgac agagagacag gctgtgatac aatggcggtg
     1441 agtccgcgga ggtggaggaa agcatggatc aggtgcagag cagtgcagga catgcaacgg
     1501 ggccgatcga ggccaaaccg gggaaatacc tcacctatcg gcttggtggt gaacagtacg
     1561 gcctggagat catgaaggtg caggaaatca tcggactgat gccgatgacc cgtgt
//
LOCUS       DAOSHE010000472         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_79369_length_1603_cov_3.731912, whole genome shotgun sequence.
ACCESSION   DAOSHE010000472 DAOSHE010000000
VERSION     DAOSHE010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79369_length_1603_cov_3.731912"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLD82_10195"
     CDS             <1..536
                     /locus_tag="PLD82_10195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016710493.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG65793.1"
                     /translation="TRNGFITSIQAVYVPADDITDPAAAVTFSHLDATTVLSRDIVAI
                     GIYPAVDPLQSGSRILEPSVVGKEHYETAQAVKKVLQRYNELKDIVAILGVSELPKED
                     ELVVNRARKIQRFFSQPFSVAETFTGLPGRYVEINETIAGFKAILAGEVDDLPEQAFY
                     MVGTLDEARQRAREMAQ"
     gene            533..796
                     /locus_tag="PLD82_10200"
     CDS             533..796
                     /locus_tag="PLD82_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65794.1"
                     /translation="MMRCTITSPITSAVFEGLAAIRVFTTTGAREILPGHIALIAGLA
                     PDSSVELVPADGSTPLHIGVAAGSFFQFEQDDALILTPSMTNG"
     gene            1098..>1603
                     /locus_tag="PLD82_10205"
     CDS             1098..>1603
                     /locus_tag="PLD82_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOG65795.1"
                     /translation="MNTITLTLFTILSGTYLVLSILAGQAPEFPAVIIKAAATASLLF
                     ATVRTLLHTKSGGRINKPLAIVLVGLPWCIAGDVFLGIPGDKFFLPGLASFLIGYAIF
                     AVSLVSRLSLLRYGLMLIPALIPGGLFLAWFTLPQDLVWPVRIFIAVQTAFLAAGFSM
                     LRSRAGLAA"
BASE COUNT          324 a          525 c          415 g          339 t
ORIGIN      
        1 caacacgaaa cgggttcatc acgtccattc aagccgtcta tgtcccggcc gatgacatca
       61 ccgacccggc cgctgcggtc accttcagcc acctggatgc tacgaccgtg ctctcgcgcg
      121 acatcgtggc catcggtatc taccctgccg tcgatccgct ccagtcagga tcccgcatcc
      181 ttgaaccctc cgtggtcggc aaggagcatt atgaaacagc ccaggcggtc aaaaaagttc
      241 tgcaacgcta caacgaactc aaggacatcg ttgccatcct cggtgtcagc gaacttccca
      301 aggaagacga acttgtagtg aaccgcgcac gcaagatcca gcgctttttc tcccagccgt
      361 tttcggtggc agaaaccttt accggactcc ccggacggta cgtcgagatc aacgagacga
      421 tcgcaggatt caaggccatt ctggccggcg aagtcgatga tctgccggaa caggccttct
      481 atatggtagg aacccttgac gaagccagac agcgcgcccg ggagatggcg caatgatgcg
      541 ctgcacgatc accagcccga tcacgagtgc ggtctttgaa ggtctcgccg cgatccgggt
      601 cttcacaacc accggggcga gggaaatcct cccgggccat atcgcgctga tcgcgggtct
      661 ggcgcctgat tcgagcgtcg aactcgtccc ggcagacggc agcacgccac tccatatcgg
      721 tgtcgccgcg ggcagttttt tccagttcga gcaggacgat gccctgatct tgacaccatc
      781 gatgacaaac ggctaaggga ctgactccgg accagcgtcc cttggtttga gcaacaacac
      841 ctggattgca atacgatcca aatgacatca tccgggggga agcccccccc gcgcccgagc
      901 cgcgaacggg cagactggat gaacgcggcc ggcaacaaaa ccaaccagcg gttatccgcg
      961 tctcgggctc ccctctcgca tgagaccaaa aaacacccgg catgctatcc agccattcgt
     1021 gccaccaccc tgacgtgcta cccaattatg tagaaaatga ctggccagac atggtctggc
     1081 gggtatagtc ttgcggtatg aacacgatca cactgacact cttcaccatt ctgtccggaa
     1141 cctatctggt actctccatc ttggccggac aagctcccga atttccggcc gtcatcatca
     1201 aagcggccgc gaccgccagt cttctttttg cgacggtccg gacactcctc catacgaagt
     1261 ccggtggccg gatcaacaaa cctctggcca ttgttctggt tggtctcccc tggtgtatcg
     1321 ccggagatgt ctttttggga atccccggtg acaaattctt tctccccgga ctggcctcat
     1381 ttctgatcgg gtatgccatt tttgccgttt ccctcgtgtc acggctttcc ctgctgcgct
     1441 atggtctgat gctcatcccg gccctgatcc ccggcggcct gtttctcgca tggttcactc
     1501 ttccccaaga tctcgtctgg ccggtccgga tcttcattgc agtccaaact gccttcctgg
     1561 ccgcgggatt cagcatgctc cgcagcaggg ctggcctggc cgc
//
LOCUS       DAOSHE010000473         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_79437_length_1602_cov_4.405301, whole genome shotgun sequence.
ACCESSION   DAOSHE010000473 DAOSHE010000000
VERSION     DAOSHE010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79437_length_1602_cov_4.405301"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1231)
                     /locus_tag="PLD82_10210"
     CDS             complement(281..1231)
                     /locus_tag="PLD82_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG65796.1"
                     /translation="MLEVVQLEKRFPVRNGFLRKPSAWVHAVSEVSFSLGPRESLGLV
                     GESGSGKTTIGRCLVRMEDPTAGMILLNGVDLAALDQNTLKPWRRKVQYVFQDPYSAL
                     NPRMKVGASVAEGLHVHTSLKKGEIQHRVEELLQNVGLSADAMSKYPHEFSGGQRQRI
                     AIARALILDPELLVLDEPVSALDVSVQAQILNLLEDIRDQRSLATVLISHNLAVVRQV
                     TDRTAVLYLGRIVESGPTGQVLEKPRHPYTASLIQSIPEAGKPIVPSIEGEVPSNITL
                     PSGCPFHPRCPQARTGVCDRELPQLSGDAGHWAVCHFPIG"
     gene            complement(1224..>1602)
                     /locus_tag="PLD82_10215"
     CDS             complement(1224..>1602)
                     /locus_tag="PLD82_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HOG65797.1"
                     /translation="REGSMSILFITHDLGIVGQLTDRVVVMYAGRVAESGPSERVLTR
                     PLHPYTAGLLASMPGMHRQGGRLAAIPGRVPSPVEFPGMTGCRFAPRCPLADERCRLE
                     VPVLADRDGVEVACHHAGRATDA"
BASE COUNT          358 a          492 c          460 g          292 t
ORIGIN      
        1 acttgagatt ccggacaccg accaggtcga gcttccagag gaggagagca gactggactg
       61 actggatgaa gacagcggag tggcctcatc atccccgcag ccggtccccg ccagcccaaa
      121 aacaaacatc catacaccaa ccaagaccat tgccatacag cgcattccca tatcttgacg
      181 cctctccttc ttctggatcc ggtacgaacg gccaaccgtc acggtgaaat catatcacaa
      241 tattcatttt tttctcgaaa tatgaccgtt tccggctcgt tcatccaatc ggaaaatggc
      301 agacagccca gtgtccggca tcaccggaga gttgcgggag ttcccggtca cagacgcccg
      361 ttctggcctg tgggcaacgg ggatggaagg gacacccgga gggcagcgtg atattggacg
      421 gaacctcccc ttcgattgag gggacaatcg gttttcccgc ttccggaatg gactggatca
      481 atgaggctgt atagggatgt cgcggcttct cgagaacctg cccggtcggg ccgctttcga
      541 cgatcctgcc gagatagagg acggccgtcc ggtccgtgac ctgacggaca accgcgagat
      601 tgtgcgagat gagaaccgtc gccagactgc gctggtcccg gatatcctcc aacaggttga
      661 gaatctgcgc ctgaaccgag acatccagtg ccgaaacagg ttcatcgagc acaaggagtt
      721 cgggatcgag gatcagggcc ctggcaatgg caatccgctg ccgctgccct cctgaaaatt
      781 cgtgggggta tttcgacatg gcgtcagcgg aaagacccac attctgcagc aattcctcga
      841 cccggtgctg gatctcccct tttttcaagg aggtgtggac atgcagcccc tcggcgacac
      901 tcgcaccgac cttcatccgg ggattcaagg cactgtaggg atcctggaag acatactgga
      961 ctttccgcct ccagggtttg agtgtattct ggtcaagagc ggccagatcc accccgttca
     1021 ggaggatcat cccggccgtc gggtcctcca tccgtacgag acagcggccg atggtcgttt
     1081 tccccgagcc ggattcgcca accagaccga gggactccct tggtccgaga gagaaactca
     1141 cttcactgac ggcatggacc caggccgagg gcttgcgcaa gaacccgttg cgcaccggga
     1201 aacgcttttc aagctggaca acttcaagca tcagtcgccc tccccgcatg gtggcaggcc
     1261 acttcgaccc cgtccctgtc ggcaaggacc gggacttcga gcctgcagcg ttcatcggcc
     1321 aggggacagc ggggggcaaa acggcagccc gtcatccccg gaaactcgac cggagaggga
     1381 acacgaccgg gaatggcggc caagcggccg ccctggcgat gcatcccggg catcgacgcc
     1441 aggagtccgg ctgtataggg atggagcggc cttgtcaaaa ccctctccga tgggccggac
     1501 tccgccaccc gaccggcata catgacaacg acacgatcgg tcaactggcc aacgattccc
     1561 agatcatggg taataaagag gatcgacatg gaaccttcac gc
//
LOCUS       DAOSHE010000474         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_79519_length_1601_cov_3.482536, whole genome shotgun sequence.
ACCESSION   DAOSHE010000474 DAOSHE010000000
VERSION     DAOSHE010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79519_length_1601_cov_3.482536"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLD82_10220"
     CDS             complement(<1..1160)
                     /locus_tag="PLD82_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65798.1"
                     /translation="MFTSTMMFVKMTVIAVTTVFLLSEASRRRDRRALWMGLALVLFI
                     IKDAVLLYLLQRPPAAGVPVLGEFFNNLVSYGLPLGLNVPLPVEVFHHYFLVWAGAEM
                     LFAVVLQQALSSLALEGRDRLVQLLVLLGNFIIVVIFGILAFTITEYQQFSDMMTYYL
                     WAVVWAGVQYIVILGRTMLAKHGDGLSLNFCVRYRVFLVSVAVFSIVQVATIILIPLR
                     AFETIPFSVYAILWHLTGFLWILVAWLYQRHSLSNADLEAEGRLLQQKLYREMGFSVA
                     ETLSMQTIVEMLAKNGQEILDAETCGLFLVDEQGERVSVGSLTGFFPPVEPVKIEENT
                     TPEMLMSRFRANRFRVGKTWVGEVAASGQPLEVLDNLKTSRIPQTGGKWCTV"
     gene            complement(1253..>1601)
                     /locus_tag="PLD82_10225"
     CDS             complement(1253..>1601)
                     /locus_tag="PLD82_10225"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG65799.1"
                     /translation="GIDMFDCVLPTRNARNASLFTMDGPVSMRNARFKTDFSPLDPEC
                     DCETCRNYSRAYLHHLYRTGEILASMLGTMHNLAFLWRLMQFMREALREDRFAEFRAG
                     FIARYQARGQKPG"
BASE COUNT          404 a          458 c          447 g          292 t
ORIGIN      
        1 ctgaccgtac accatttacc gccggtctgc gggatccgcg atgtcttgag gttgtcgaga
       61 acctcgaggg gttgcccact tgccgcgacc tctccaaccc aagtcttgcc gacccggaac
      121 cgattggccc tgaagcggga catcagcatc tccggagtgg tgttttcctc gattttgacg
      181 ggctcgaccg gagggaaaaa accggtcagg gaaccgaccg aaacccgctc cccctgttcg
      241 tcaaccagaa agaggccaca ggtctccgca tcgagaatct cctgtccgtt tttcgccagc
      301 atttcgacaa tcgtctgcat cgagagggtt tcggcgacac tgaaccccat ctcccgatac
      361 agtttctgct ggaggagccg tccctcggcc tcgaggtcgg cattggagag actgtgtcgt
      421 tgatagagcc aagccacgag aatccagagg aatcccgtca gatgccagag gatcgcgtag
      481 acactgaacg ggattgtctc gaaagctcgc agggggatca ggatgatggt tgcaacttgc
      541 acaatgctga aaacggccac tgaaacgaga aagacccgat atcgcacaca gaaattgagg
      601 gaaagtccgt cgccgtgctt cgccagcatt gtcctgccca ggatgacgat gtactggacc
      661 ccggcccaga cgacagccca gaggtagtac gtcatcatat ccgagaactg ctgatactcg
      721 gtgatcgtaa aagccaggat cccgaaaatg accacaatga taaaattccc gagcaggacc
      781 agcaactgca caagcctgtc tcgtccctca agggccagag aagagagcgc ctgctgcaga
      841 acgacggcaa aaagcatttc ggcaccggcc cagaccagga agtaatggtg gaagacttcg
      901 actggaagag gaacattcag tccgagcggc agtccgtaac tcacgagatt gttgaaaaat
      961 tcaccgagga cgggaacgcc cgccgctggt gggcgctgca gcaagtagag gagaacagcg
     1021 tcctttatga tgaaaagaac cagagccagt cccatccaga gcgcacgcct gtcgcgtctg
     1081 cgggaagcct cggagagaag aaagaccgtt gtgacggcga tgacggtcat cttgacaaac
     1141 atcatggtag atgtgaacat atcacctgac cgccgaactt ggactgaatt cgggaatcaa
     1201 tcttgatccc caagagatca taccctgccc ggagcccgcc gtcaatgaaa attcacccgg
     1261 gcttctgtcc ccgcgcctga tagcgggcaa tgaacccggc acgaaattcg gcaaaacgat
     1321 cttcgcggag cgcctcccgc atgaactgca tcagacgcca gagaaacgcc agattatgca
     1381 tggtccccag catgctggcc aggatctccc cggtccggta gaggtgatgg aggtaggccc
     1441 tgctgtagtt ccggcaggtc tcgcagtcgc actccggatc cagcggggaa aaatccgtct
     1501 tgaagcgcgc attgcgcatc gaaaccggtc catccattgt aaagagggag gcattccggg
     1561 cattccgtgt cggcagaaca caatcgaaca tgtcgattcc g
//
LOCUS       DAOSHE010000475         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_80370_length_1590_cov_3.100326, whole genome shotgun sequence.
ACCESSION   DAOSHE010000475 DAOSHE010000000
VERSION     DAOSHE010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80370_length_1590_cov_3.100326"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    282..291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            401..1366
                     /locus_tag="PLD82_10230"
     CDS             401..1366
                     /locus_tag="PLD82_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65800.1"
                     /translation="METLNFKGVDYRVEPISSKAEFDALYRDLLRKVNSGKSILKDLE
                     KAYRISPSEVYLVRYDQERRALVPVRIAKESVDGLLIRNRYDDKAKTLQRGIGQEMAK
                     HTQDIYDAVEKKKQLNEKNEKQIAERRAQEQKLVEIDGVNFQERLVWHRTVIGEIQGI
                     LRQHDMKYREDRDYKGKIDMAFEKIIRQVEQFEAGMKDIERDNGKSVSAVRHLLPADI
                     SKDNPPEGYVRMLQTYLSVAALNVQLEKEWTTLGEYVLDMAGLPVKVAQLVQMVGELI
                     NRTRLLTGAAERILFPEKYSFRRDSNAVYDFSKLSKDVVAILMHQ"
     gene            complement(1372..>1590)
                     /locus_tag="PLD82_10235"
     CDS             complement(1372..>1590)
                     /locus_tag="PLD82_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOG65801.1"
                     /translation="AIALALRFGAPLFVSKLVLQTATVPLPDEEPKPESMDPVGELKK
                     ELELAISEERYEDAARLRDELKKINNEN"
BASE COUNT          391 a          378 c          465 g          346 t
ORIGIN      
        1 acgagaattg ccgggtgacg gtcggtatga atgatgtatt gtatgccgct gaaagggtgc
       61 cggacaggag gacgagcagg atcagcaagc caatgcagta tcgcacggta caacgctccg
      121 atcttttttg cacaatggcc aactctccct aatataacac atttgggtga tatccatcat
      181 cgtctgcaga atccctgcag aaaggagaga aggtcttttt cgggcctggc gatggttgct
      241 gccccgttgt tttcatgaac cccgtgtgtc gctcgaggtt gnnnnnnnnn nggttgcact
      301 tgaatgcatt gggggaacgc aagcaggaac tcaaggttga cgaatcaccc gggacgcgct
      361 accatgaacg ctatgaccgg cttgcatggc agggggaacc atggaaacgc tgaatttcaa
      421 gggtgtcgac taccgtgttg aaccgatctc gagcaaggcc gagtttgatg cgctctatcg
      481 tgatctcttg cgaaaagtca attcagggaa atccatcctc aaggatcttg aaaaggccta
      541 tcggatctca ccatccgaag tctatctcgt cagatacgat caggaacgtc gcgcactcgt
      601 tccggtcagg atcgccaagg agtcggtcga tggccttttg attcgcaaca gatacgatga
      661 caaggccaag acccttcaac gggggatcgg gcaggagatg gccaagcata cccaggacat
      721 ctacgatgcc gtcgaaaaga agaagcaact caatgaaaaa aatgagaagc agatcgcaga
      781 gcgaagggct caggaacaga aactggtcga gattgacggg gtgaatttcc aggagcggct
      841 ggtttggcac aggactgtca tcggtgagat ccagggaatc ctcagacagc acgacatgaa
      901 gtacagggag gaccgggatt acaaaggcaa aatagacatg gcctttgaaa agatcatccg
      961 ccaggttgaa cagttcgagg ccgggatgaa ggatatcgaa cgcgacaatg gcaagagtgt
     1021 ctcagcggtg cgccatcttc ttcccgcgga catcagcaag gataacccgc cggaagggta
     1081 tgtccggatg ctgcagacct atctgtctgt ggcggccctc aatgtccagc ttgaaaagga
     1141 atggacaaca ctcggggaat atgtgctcga tatggcaggc ctcccggtca aggtggcaca
     1201 gctcgtgcag atggtgggag aattgatcaa caggacacgt cttttgaccg gcgcggccga
     1261 gcggatcctc ttccccgaga agtactcgtt caggcgcgac tctaatgcgg tctacgattt
     1321 ctcgaagctc tcgaaggatg tggttgccat cctgatgcat cagtgatttg atcaattttc
     1381 gttgttgatt tttttgagct cgtcacggag acgggctgcg tcctcgtagc gttcttcgct
     1441 gatggccagt tcgagctctt ttttcaattc cccgacggga tccatgcttt caggcttggg
     1501 ttcttcatcg gggaggggga ctgttgcggt ctggagaacc agcttgctga caaagagggg
     1561 cgctccaaaa cgcaaggcga gggcgattgc
//
LOCUS       DAOSHE010000476         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_80911_length_1583_cov_3.939136, whole genome shotgun sequence.
ACCESSION   DAOSHE010000476 DAOSHE010000000
VERSION     DAOSHE010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80911_length_1583_cov_3.939136"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLD82_10240"
     CDS             complement(<1..1302)
                     /locus_tag="PLD82_10240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG65802.1"
                     /translation="MRIAFHGAAGSVTGSNHLIQTEGGTLMIDCGMFQGPKEIRTRNH
                     MAFAYQPQTIDAVIITHAHIDHSGRLPRLVKQGYSGPIYATPATAELLRILLLDSAHI
                     QMSDSSWENKKRKRKGQELVQPLYDDQDVEATLKLVRAVDYHTPFQILPNVRATFHDA
                     GHIMGSSFLELAITEHGITRTLIGGGDLGRSHQAIIGDPEVGDRADMVMIESTYGNRR
                     HKNEEDTNAELIAVLKEVVETRGTLLIPAFAVGRTQEMLYRLFELSQSNSIPAMPVFV
                     DSPMAREVTRIYELHKDLYDEKTLEYVRLGKNPLRTPDLHFTESVEDSMSLNDVPGPK
                     IILSASGMCDAGRIVHHLKHNIWQKNCHILFVGYQAEGTLGRRIIEGAKRVSILGETL
                     QVNAQIHTIGGLSAHADSAEMLAWLKFYQQSNPTVFLVHGDP"
BASE COUNT          321 a          434 c          477 g          351 t
ORIGIN      
        1 gggatccccg tggacaagaa acactgtcgg gttggactgc tgataaaatt tgagccaggc
       61 cagcatctcg gccgaatcgg catgtgccga aaggccgccg atagtgtgga tctgggcatt
      121 cacctggagc gtctccccga gaatactgac tcgcttggcc ccctcgatga tgcgccgccc
      181 cagcgtgcct tccgcctggt acccgacaaa gaggatgtga cagttcttct gccagatatt
      241 gtgcttgagg tgatggacga tacggccggc gtcgcacatc cccgatgccg agaggatgat
      301 cttgggtccc gggacatcgt tcaaggacat cgaatcctcg acggattccg taaaatgaag
      361 atccggggtc ctgagcggat tttttccgag ccggacgtat tcgagggtct tttcgtcgta
      421 gagatcctta tgcaactcgt agatgcgggt gacctcgcgg gccatcggac tgtcgacaaa
      481 gacgggcatg gcggggatgc tgttggactg cgagagctcg aacaagcgat agagcatctc
      541 ctgggtacgg ccgacggcaa aggccggaat gagcagtgtg cccctggttt cgacaacctc
      601 tttgagaact gcaatcaact cggcgttggt gtcctcttca tttttgtggc gccggttccc
      661 atacgtggat tcgatcatga ccatatcggc ccggtcaccc acctcgggat ccccgataat
      721 ggcctgatgt gaccgcccaa gatccccgcc tccgatcagg gtgcgcgtga tgccatgttc
      781 ggtgatggcc agctccaaaa aactgctccc catgatgtgc ccggcgtcat ggaaggtggc
      841 acggacattc gggagaatct ggaaaggcgt gtggtagtcc acagcacgga cgagcttgag
      901 tgtcgcttcg acatcctggt cgtcatagag cggctggacc agttcctgcc ctttgcgttt
      961 tcgtttcttg ttttcccaac tgctgtccga catctggata tgggccgagt ccaagaggag
     1021 gatacgcaac aactccgcgg tggccggggt ggcatagatc gggcctgagt acccctgctt
     1081 gacgagcctg ggcaagcggc cggaatggtc aatatgggcg tgggtgatga tgacagcatc
     1141 aatcgtctgg ggttggtagg cgaaggccat gtggttccgg gtccggatct ctttcggccc
     1201 ctggaacatc ccgcagtcaa tcatcagggt tccgccctcg gtctggatca gatgattgct
     1261 tccagtcacg gatccggctg caccatggaa tgcgatacgc atacagagct ccttgcaacg
     1321 gttgatttcc acacggagtc gttgtccggg attgagtgta gcacggatcg gaatatcatg
     1381 ccaccaggaa tgcctggggg attccttccg ggagagccca tccatgggaa gcagaattcg
     1441 tttgaaattg gttcgctgtt tcagtccgga ccgtcgtgca aggcgtgcgg aaccagcagg
     1501 agcatcccgc ttgcattgcc cgccttcatc ccctaacatt atgcaaatgc tgacactcgg
     1561 atacacaaag tgccacgggg ccg
//
LOCUS       DAOSHE010000477         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_81185_length_1580_cov_2.739672, whole genome shotgun sequence.
ACCESSION   DAOSHE010000477 DAOSHE010000000
VERSION     DAOSHE010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81185_length_1580_cov_2.739672"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLD82_10245"
     CDS             complement(<1..499)
                     /locus_tag="PLD82_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65803.1"
                     /translation="MRILFLICFVVLGIHQIHSRTVLAPALDGADGSKEEDATEGIIV
                     EEFKQTGDYIIKHAGGNKVTLQISRHHITATNRFLTAIIRDLRENRHMLTGMRLTVAP
                     HPGKKLVQERILVRFKDPVHIPYTTLRLFCWYQQDRPGSIDALTLNCTDIYGIDFDVY
                     HSQSLG"
     gene            complement(496..1143)
                     /locus_tag="PLD82_10250"
     CDS             complement(496..1143)
                     /locus_tag="PLD82_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65804.1"
                     /translation="MWCRTIFGFLIVWFVPIFVAGSWETAEEEYYYVLIDDFEQPVQW
                     TGSVVAQGRTNTLIIQRIENTVEARKRIEKYATMFARDLSPASRAVSPRQNFVLGIKD
                     IITSPGERILRIRPRQPVRIENVRSLNFYLQKDKHDFVLYPVILDVDKSRLPTRGTYC
                     RYNGWRRMMIPLFESFYDGAAWFDGFEIRIAPWISDTVLYLYFDLLVIHSARDVQ"
BASE COUNT          412 a          447 c          365 g          356 t
ORIGIN      
        1 taccgagact ctgagagtga taaacatcaa aatcaatccc gtagatatcc gtgcaattca
       61 gcgtcaaggc atcaatactc cccggtcgat cctgctggta ccagcaaaag agtctaaggg
      121 ttgtatatgg aatgtgcacc ggatccttga atcggacaag gatccgctcc tggacgagtt
      181 ttttcccggg atgtggcgca actgtgagcc gcatcccggt cagcatgtga cggttttccc
      241 gcaggtcccg aatgatggct gtcagaaacc ggttggtcgc ggtgatgtgg tgccgggaga
      301 tctggagcgt gaccttgttg ccaccagcgt gcttgatgat ataatcgccg gtttgcttga
      361 actcttcaac gatgatgcct tcggttgcat cctcttcttt cgagccgtca gccccgtcca
      421 gagcgggtgc gagcactgtc cgggaatgga tttgatggat tcccaaaacg acaaaacaga
      481 tcaagaacaa gattctcatt gcacgtccct tgccgagtgg attaccagga ggtcaaaata
      541 gagatacaag acggtgtccg atatccatgg tgcaatccgg atctcaaagc cgtcaaacca
      601 ggccgcccca tcgtaaaatg actcaaacag cggaatcatc atcctgcgcc aaccattata
      661 ccggcaatag gtccctcttg tcggcaacct ggacttgtcg acatcaagaa ttacgggata
      721 gagcacaaag tcgtgtttgt ctttttgcaa gtaaaaattc aatgagcgaa cattctcgat
      781 cctgaccggt tgccttggcc ggatgcggag tatccgttca cccgggctcg taatgatatc
      841 tttgatcccg agaacaaagt tttgacgggg tgaaacagcc ctggacgccg gactgaggtc
      901 ccgtgcaaac atcgtcgcat atttttcaat cctttttctc gcttccacgg tattttcgat
      961 gcgctggatg atcagagtgt ttgtccgacc ttgtgctaca acactccccg tccactggac
     1021 cggttgctca aaatcatcta tcaagacata atagtattct tcttccgcag tttcccatga
     1081 acctgcaaca aaaatcggta caaaccagac aatcaagaat ccgaaaattg ttctacacca
     1141 cataggaacc tctccatccc gaatggtcat ggcacaacag ccccaatgca tttccagtat
     1201 agccttggct gccttctatt caaagcgaaa aattcacaag aatctgccat agaattcatc
     1261 ttccaccccg cgcccccaaa acaaccaggc agttcacaat cgtgggcaag gaatacagag
     1321 gaacacagcc agaccgaact gtgcccgcca ccccgccgtt gccgcgtcat ccattgtctg
     1381 acgtcccaca agaacggcac cgacatcggc agaccggtgc cagcagcgat atccatacac
     1441 tggcgatgaa caaagaccct gcatgccggt tcacgatccg tacgcagcac tgcagcagca
     1501 aaagtccccg accgacatcc cgttcacatc cggcgggcca cccggagacc ataattgttt
     1561 ccaccaccat aggggaaatt
//
LOCUS       DAOSHE010000478         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_81415_length_1577_cov_2.798949, whole genome shotgun sequence.
ACCESSION   DAOSHE010000478 DAOSHE010000000
VERSION     DAOSHE010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81415_length_1577_cov_2.798949"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1335)
                     /locus_tag="PLD82_10255"
     CDS             complement(31..1335)
                     /locus_tag="PLD82_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65805.1"
                     /translation="MTHRITLLLAAAGIVFLFPGFSSAQAIDENTLFSNTNTMIIDTG
                     RITDASLHPGTSKKGVSTSGTLRSRTTYTMSESWFEGASEQVNSLFQFFEGDFLLDVR
                     LPSGIKSLVAVSLWQTPQGLADSSRPGTTNTYGWTIKEAFVDLAASGNLSFRIGKQVL
                     QWGRSYFWNPVDLVNYEKKNFLDMDRSREGSTGVRTHAAFGSTLNFYTWIDAGDARSG
                     SDLAIAGKIEFVAGGSEFALTVWGKKGFIPVYGLELSSRLLGFDIKGECALSRGSNTP
                     RYTRQGMGVTEEDRSGDWVPQASLTLSRGIDWEIDDRIQIVAELMANGAGYSDNVLAD
                     PLRSAAIYLNDAWTPNWLSRWYAALFVSVAKFPDSTMTLQANLMANLVDGSMIVSSGI
                     SWQPVNDLTLMPMLYATVGKEGREYTIRGRNLATEFQARIAF"
BASE COUNT          301 a          432 c          486 g          358 t
ORIGIN      
        1 tgacatattg tctgactccg ggacgctcga tcagaaggca attcgtgcct gaaactctgt
       61 tgcaagattt cttccccgga tggtgtattc ccgcccttct ttgccaaccg ttgcgtagag
      121 catcggcatg agtgtcaggt cgttgaccgg ctgccagctt atcccgctgc tgacgatcat
      181 cgaaccgtcg acaaggttgg ccatcagatt tgcctggagt gtcatcgtgc tgtcggggaa
      241 ttttgccacc gagacaaaga gcgcagcgta ccagcgcgag agccagttgg gggtccaggc
      301 gtcattgagg tagatggcgg cgctgcgcag cggatccgcc aggacattgt cactgtagcc
      361 cgccccgttg gccatcagtt cggccacgat ctggatccgg tcatcaattt cccagtcaat
      421 cccgcgcgag agggtcaagg aagcctgcgg tacccagtcc ccgctccggt cctcttcggt
      481 aacacccatt ccctgtcttg tgtagcgcgg ggtgttgctt ccccgtgaga gtgcgcactc
      541 acccttgatg tcgaatccaa gcaggcgtga ggagagttcc agaccgtaga cagggatgaa
      601 gccttttttc ccccagacgg tcagggcgaa ttcgctgccg cccgccacaa attcgatttt
      661 accggcaatg gccaggtccg aacccgagcg tgcgtcaccc gcatcgatcc aggtgtagaa
      721 gttgagggtt gagccgaagg cagcgtgggt acgcacgcct gtggatccct cgcggctcct
      781 gtccatgtcc agaaagtttt tcttttcgta attgaccaga tcgaccgggt tccagaagta
      841 actccggccc cactgcagga cctgcttgcc gatcctgaag gagaggttgc cggatgcggc
      901 gaggtcaacg aaggcttcct tgatcgtcca gccgtaggtg ttggttgtgc ctggcctgct
      961 gctgtcggcc aggccctgcg gcgtctgcca gagcgagacg gccacgaggc tcttgattcc
     1021 cgagggcagg cggacatcca gcaggaagtc cccctcgaaa aactggaaga ggctgtttac
     1081 ctgctcggat gcaccctcga accaggattc gctcatggtg taggtcgtcc ggctgcgcag
     1141 ggtgccgctt gtcgaaacgc ccttttttga ggttccggga tgcaatgatg cgtccgtgat
     1201 tcttccggta tcaatgatca tggtattggt attcgagaat aacgtgtttt cgtcgattgc
     1261 ttgggcactg gaaaatccgg ggaacaagaa aacaataccg gctgcggcaa ggaggagggt
     1321 gatcctgtgt gtcatggctc ggttccttgg aatgggaatg ttcgccggac tggttttgtt
     1381 catggtgaac attctccaat ccggcttgtg cgcggaaaat ccgtgtaacg ggggctcagc
     1441 ggctcaggtt ttcgagatac gccttggtga agacatgtcc cggtatcggt gccagcgaga
     1501 ttccggagat ctcgagcagg gtgccatgga cctcgatgga gccggccgag ggggcgtcga
     1561 tgcagccgat caggttg
//
LOCUS       DAOSHE010000479         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_81934_length_1570_cov_3.201320, whole genome shotgun sequence.
ACCESSION   DAOSHE010000479 DAOSHE010000000
VERSION     DAOSHE010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81934_length_1570_cov_3.201320"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLD82_10260"
     CDS             <1..327
                     /locus_tag="PLD82_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOG65806.1"
                     /translation="RTDDMLIIRGVNVFPSQIEEVLLRFESIQPHYQLIVDRKEHLDN
                     LEVQIEMTSELFSDEVRDLEGIEHRIESEIASALGIHAKVRLVEPKTIPRSEGKAVRI
                     IDKRKI"
     gene            340..771
                     /locus_tag="PLD82_10265"
     CDS             340..771
                     /locus_tag="PLD82_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOG65807.1"
                     /translation="MSVTQVSVFIENREGRLAEVSRLLSGKGINIRSLSLADSSDFGV
                     LRLIVDNPEQCIQVLQENGFVARATPVLALEIEDRPGGLDRVLSLFSSNGINVEYMYA
                     FVEKSHDNAIVIFKTGDNGRALDLASREGIPVIRGEALFSR"
     gene            790..>1570
                     /locus_tag="PLD82_10270"
     CDS             790..>1570
                     /locus_tag="PLD82_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG65808.1"
                     /translation="MESSCWQHEIETMEPHALRAMQLERLNAVLARAAKSPYYGKLFS
                     QNGLGRIGSLDEFSRYPFTGKQDLRDNFPYGFLTGSLDETVRLHSSSGTTGIPTVVFY
                     TPEDLDVWTECVTRSMYMTGARKCDVFQNMMGYGLFTGGIGFHYGAERLGMLTIPSGP
                     GNSKRQIWFMKTFRTSVMHILPSYALHLMTYFPEEGIDPRRDLALKIAFIGAEPHSEG
                     VRTKIEEFYGIKAYNSYGLSEMCGPGVAFECPAQEGLHVWED"
BASE COUNT          351 a          416 c          444 g          359 t
ORIGIN      
        1 agaacggatg acatgttgat catccgtggc gtgaatgtct ttcccagcca gatcgaagag
       61 gttcttctcc gcttcgagag tattcagccg cattatcagc tcatcgtgga ccgcaaggaa
      121 catcttgaca acctcgaggt ccagatcgag atgacctccg aactcttctc cgacgaggtc
      181 cgtgaccttg aggggatcga gcacaggatc gaaagcgaga ttgcctcggc gctcgggatc
      241 catgcaaaag tgcgactggt tgagccaaag acgattccgc gcagcgaagg caaggctgtg
      301 cgcatcattg acaaacgaaa aatctgatcg ggggacggca tgtcggtaac acaggtttcg
      361 gtttttattg aaaaccgtga gggcaggctg gccgaggtca gccgactcct ctccgggaag
      421 ggcatcaata tccgttcgct ctccctggcc gattcatcgg atttcggagt cttgcggctg
      481 atcgtggaca atccggagca gtgcattcag gtcctgcagg aaaacggatt tgtggccagg
      541 gctacccctg ttctggccct cgagatcgaa gacaggccgg gtggactgga tcgcgttctt
      601 tccctttttt cctcaaacgg aataaacgtc gagtacatgt acgcctttgt cgaaaaatca
      661 cacgataatg ccattgtcat tttcaagacc ggggacaacg ggagggcact cgatctggct
      721 tcgcgtgagg ggattcccgt catccgggga gaggccctgt tttcacgata acgcatcgaa
      781 aggttggtca tggaatcttc ctgctggcaa cacgagatcg aaacgatgga gccgcatgca
      841 ttgcgtgcca tgcagcttga acgtctgaat gctgtacttg caagagcggc aaagtctccc
      901 tactacggaa aactcttttc ccaaaatggg ctcggacgga tcggatcgct ggacgagttc
      961 tcgaggtacc cgttcaccgg taagcaggat ctcagggata attttcccta tggatttttg
     1021 accggctctc tggacgagac tgttcgtctc cattcttcat cgggaacaac cggcatcccg
     1081 acggtcgtct tctacacacc tgaagatctc gatgtctgga ccgagtgtgt gacccgcagc
     1141 atgtacatga ccggtgcccg gaagtgtgac gttttccaga acatgatggg ttacgggctc
     1201 tttaccggcg gaatcgggtt tcattatgga gccgagcgcc tgggcatgtt gaccatcccg
     1261 tccgggccgg ggaattccaa gcgccagatc tggttcatga agaccttccg caccagtgtc
     1321 atgcacatcc tccccagcta tgcgctgcat ctgatgacgt attttcccga ggagggcatt
     1381 gatccacgca gagacctcgc cctgaaaatc gcctttatcg gtgcggaacc ccatagtgag
     1441 ggtgtgcgca caaagatcga ggaattctac gggatcaagg cttacaactc ctatggcctc
     1501 tccgagatgt gcggaccggg agtcgctttt gagtgtccgg cacaggaagg cctgcatgtc
     1561 tgggaagacc
//
LOCUS       DAOSHE010000480         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_83697_length_1549_cov_4.044177, whole genome shotgun sequence.
ACCESSION   DAOSHE010000480 DAOSHE010000000
VERSION     DAOSHE010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83697_length_1549_cov_4.044177"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLD82_10275"
     CDS             <1..1128
                     /locus_tag="PLD82_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG65809.1"
                     /translation="ASNVAGLRHMIYFDENDVFLSVLPLHHTFECTAGMLIPVSKGCS
                     ITTAESLASKRIIANIKETGVTLMMGVPLLYEKMVAGIFRGINEKPIPVRLLVHGLLG
                     MVKGVKKLLRHNLGHKVFKSLRAKAGLGSLRIMISGGAPLQAWVGQTFEHLGISFLNG
                     YGLTETSPVLSVNVVNDIDNRTVGYPVHGIELAIDQPDPSGVGELKARGPFVMQGYYK
                     NKKATQAILRDGWLYTGDLARFDDKGRVIISGRCKNLIVTDGGKNVYPEEIELALNSS
                     TFIKETLVIGRPISKNNPGEEVVAYVVPDYETIEAAHGTKITDEEIEALVRREVNRIN
                     ESFVSYKRIKECFIREEEFPKTSTRKIKRYLFQGRDIHVRI"
     gene            1197..>1549
                     /locus_tag="PLD82_10280"
     CDS             1197..>1549
                     /locus_tag="PLD82_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65810.1"
                     /translation="MASLRDAWKGLSRVAHRIAVLVSNDSERRALESLIVSYGFLAHK
                     FPSVDAAFASRLRFDLILIDYALGHAALIDLIARLDRGSGNHYVPVLVLIDSWQVTSA
                     VPWINDGVDEFVFRP"
BASE COUNT          318 a          413 c          469 g          349 t
ORIGIN      
        1 gcttccaatg tcgccgggtt gcggcacatg atttatttcg atgaaaacga tgtctttctc
       61 tcggtccttc ccttgcatca caccttcgag tgcaccgccg ggatgctcat tcccgtgtcg
      121 aagggctgct ccatcacgac ggccgagagc ctggcgtcca aacggatcat tgccaatatc
      181 aaggagaccg gggtgaccct gatgatgggg gttcccctgc tgtacgaaaa gatggtcgcc
      241 ggaatttttc gcgggatcaa cgaaaagccg atcccggtcc ggctcctggt gcacggtctt
      301 ttagggatgg tcaagggtgt caaaaaactt ctgcggcaca acttgggaca caaggtcttc
      361 aagtcgttgc gcgccaaggc gggcctcggg agcctcagga tcatgatttc cggcggagca
      421 ccgctccagg cctgggtcgg gcagaccttc gagcatctgg ggatcagttt tctcaacggg
      481 tacggattga cggagacctc accggtcttg tctgtcaacg tcgtaaacga tatcgacaac
      541 aggacggtcg ggtatccggt ccatggtatc gaactggcca tcgatcagcc cgacccgagc
      601 ggtgttggcg agctcaaggc cagagggccc tttgtcatgc agggctacta taaaaacaag
      661 aaggccaccc aggccattct ccgggatggc tggttgtaca ccggtgatct ggcccgcttt
      721 gacgacaagg gcagggtgat catcagcggc cgttgcaaaa acctgattgt gacagatggc
      781 gggaaaaacg tctatcccga agaaatcgag ctggcgctca actcctcgac cttcatcaag
      841 gagaccctgg tgattggccg tccgatctcg aagaacaacc cgggcgagga ggtcgtggcc
      901 tatgtcgttc ccgattacga gacgattgag gcggcccatg ggaccaagat cacggatgaa
      961 gagatcgaag cgctggtccg gcgcgaggtg aaccggatca acgagagttt tgtttcgtac
     1021 aagagaatca aggagtgctt tatccgggaa gaggagttcc cgaaaacatc gacccgcaag
     1081 atcaaacgct atctcttcca gggacgggat attcacgtcc gcatctgagt cggggtgcgt
     1141 cccggtgcgc tgatatccgg cttgtcgggg tgttgtatac ggagcatgaa attggtatgg
     1201 cgtccctgag ggacgcgtgg aagggattgt ctcgagtggc ccacaggatc gcagtccttg
     1261 tgtccaatga ttcggagcgc cgcgctctcg aatctctgat cgtgtcctat ggttttttgg
     1321 cgcacaaatt tccgtcggtg gatgccgcct ttgcctcccg cctccgcttt gacctgattc
     1381 tgatcgatta tgccctgggg catgccgcgc tgattgactt gattgcccgg ttggaccggg
     1441 gctccgggaa ccattacgtt ccggtgcttg tcctgatcga cagttggcag gtcacgagtg
     1501 cggttccctg gatcaatgat ggcgtggacg aatttgtctt ccggccgat
//
LOCUS       DAOSHE010000481         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_84492_length_1540_cov_3.938047, whole genome shotgun sequence.
ACCESSION   DAOSHE010000481 DAOSHE010000000
VERSION     DAOSHE010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84492_length_1540_cov_3.938047"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLD82_10285"
     CDS             complement(<1..569)
                     /locus_tag="PLD82_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65811.1"
                     /translation="MKNKKVFILQPFSSQAASLDQPSGGDALGAAVKELFGKTGLSKI
                     TFPDDGIIELGKLGKLGEYQTLQSVSSLSGKIDSAGLQALAGALFAQGYEYVIVGMMR
                     SDGAGRLPEYRSGQGLDRSIEQVSPNVEADVAVYIISANPEEGLVFAAHARHALNDDY
                     GRTGPARALAKAYKKAFSICLKAVRARDA"
     gene            complement(862..1347)
                     /locus_tag="PLD82_10290"
     CDS             complement(862..1347)
                     /locus_tag="PLD82_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG65812.1"
                     /translation="MQIQGFGSHVVPNSPPPFCAHFAEIEVDTETGEIKVIEYVTATD
                     CGTPINPLFAEGQVEGALVNGLSYALTEGYVFNEKGVMRNPSFTTYKIFTANDIPKIT
                     TILVDGEYEKGGPFGAKSISEVAINGPIPVLANAFYHATGKRMYKTPYTPEYVLSILK
                     G"
     gene            1408..1540
                     /locus_tag="PLD82_10295"
                     /pseudo
     CDS             1408..1540
                     /locus_tag="PLD82_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          385 a          423 c          366 g          366 t
ORIGIN      
        1 tgggcatccc tcgcccgaac tgccttgaga cagatggaga aagccttttt ataggccttg
       61 gccaaggctc ttgccggacc agtacgacca tagtcatcat tcaatgcatg ccgggcatgt
      121 gcagcaaaga cgagaccctc ctcaggattg gcactgatga tatagaccgc gacgtcagcc
      181 tcgacattgg gcgagacttg ctcgatggaa cgatccagac cctgcccgga ccggtattcc
      241 ggcaagcgac cggccccgtc agaccgcatc atcccgacaa tcacatactc gtacccctgc
      301 gcgaacaatg ccccagccaa ggcttgaagt cctgccgaat cgatctttcc tgaaaggctg
      361 ctcactgatt gaagcgtctg gtattcaccc agttttccga gttttccgag ctcgataatt
      421 ccatcatcag gaaaagtaat ctttgatagt ccggtttttc caaacaactc cttgacagca
      481 gcccctaggg catcaccgcc acttggttga tccagactgg ctgcctgact gctgaatggc
      541 tgaagaatga acactttttt gtttttcatg ttggcggttg aaaacccctg tgcatgaacc
      601 gcaacttcat aactggtgga tgtacaccct gtacagaaaa gaaacaacgt catcatgaca
      661 atcaacaatt tcggtgccat acaattcctt tctgaacaca ttacgcttca ttgcatcatc
      721 ccgtcgatta tatcacaaat catgccgaac gatcctcttt ggaacaaaaa atgacgcaca
      781 aattcaatct tgctttcgat tcacatcgaa caaaaaggcc gcccggacag gacggccttt
      841 ttgtggtcag gaccaggaac atcatccctt gagaatcgag agtacatact caggagtata
      901 cggagtcttg tacatccgct tgcccgtcgc atgatagaaa gcgttggcca aaaccggaat
      961 cggtccgttg atcgcaacct cggagatcga cttggcacca aacgggccgc ccttttcgta
     1021 ctccccgtca accaggatag tcgtgatctt cggaatgtca ttggccgtaa agatcttgta
     1081 ggtcgtaaag gacggattgc gcatcacacc cttctcgttg aagacatacc cttcggtcag
     1141 ggcataggac aagccgttga ccaaagctcc ctcgacctgg ccttcggcaa agagggggtt
     1201 gatcggagtt ccgcagtcgg tcgccgtcac atactcgatg accttgatct cgcccgtctc
     1261 ggtatcaacc tcgatctcgg caaaatgcgc acagaacggc gggggcgagt tgggaacaac
     1321 atggcttccg aatccctgga tctgcatctg gttgtgtgtg tagagtgaat aggttccgat
     1381 ctcggcaaag gtcttgaatt tcgtttcatc actcttgatg gcgaccggca aaatgacccg
     1441 gcagatatcc ctaagctagt ggcgttgatt gagtcgcatg acctagtgtg cggctctcgc
     1501 gtaaatcgtc aagacccatg gataaaaaaa gtcacttctt
//
LOCUS       DAOSHE010000482         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_84530_length_1540_cov_2.639057, whole genome shotgun sequence.
ACCESSION   DAOSHE010000482 DAOSHE010000000
VERSION     DAOSHE010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84530_length_1540_cov_2.639057"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLD82_10300"
     CDS             <1..412
                     /locus_tag="PLD82_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG65813.1"
                     /translation="ATDGKVNGMRSNYPHGNVATATWTSLNGGKLGAGTSTQGYTGTC
                     FEPRADAKGDIARIYMYFAIRYYGDSRCTAWASMGAGAKLVSWSQTLYRSWSAADPVS
                     SKEKTRNEAIYTIQKNRNPLIDYPVIATLIDFTL"
     gene            complement(606..>1540)
                     /locus_tag="PLD82_10305"
     CDS             complement(606..>1540)
                     /locus_tag="PLD82_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOG65814.1"
                     /translation="KDPLARYDFFELVAGSGLASDTLAGTLWDLAWKGCVATDNFVAL
                     RQAIANDFALPSNASLALLPRGQARARWNRTRPQGGVWTVLHKKTAKGGDEPISLLEA
                     DESIRDRVRILLDRYGILFREILQHELPQSSWAQVFRSLRLLELSGEIESGQFFEGIP
                     GLQFASPLAIHQLRLPLPEDAVWWCNACDPVSPAGLRLDSLSPVEAGIRIPERRSSTW
                     LSMRGSCLVMVAKKNGRDLELGLEPDDPSLATHLALFHDLLSRENTRQRSFLVERING
                     VPAASSPWADTFLSNGFSRTWKGLELRRRIGAPV"
BASE COUNT          350 a          420 c          458 g          312 t
ORIGIN      
        1 agccactgac ggcaaggtca acgggatgcg ctccaactat ccgcatggaa acgttgccac
       61 ggccacctgg acttccttga atggtggaaa gcttggtgcc gggacatcaa cccagggata
      121 tacggggact tgtttcgagc cgagagcgga tgccaagggc gatattgcac gcatttacat
      181 gtattttgca atccgctact acggcgacag ccgatgcaca gcctgggctt cgatgggtgc
      241 aggtgccaaa ctcgtttcgt ggtcgcagac cctgtatcgc tcctggagcg ctgcggatcc
      301 ggtcagctcc aaagagaaga cccgcaacga agccatctac accatccaga aaaacagaaa
      361 tccgcttatc gattatcctg tgattgccac gctgatcgac tttacgctgt aaatcagggt
      421 tttcttgtca aaaaccgcat cggatacgcc cttgagggcg tggccggtgc ggtttttttg
      481 atgctgatca tggaaatctt cctgtgttcc acaccgggag caggaatccg aacgtggaat
      541 ctgagaagaa tttctggcag gggtccgcaa acacgtttgc ggagtgttct tgcggggagt
      601 tgtacttata ctggagcgcc gatcctgcgg cgcaattcga gccctttcca tgtccgggaa
      661 aaaccgttgg acaaaaaagt atcagcccat ggggagcttg cggccggcac gccgttgatg
      721 cgttcgacaa gaaaacttcg ctggcgtgtg ttttcacggg acaggaggtc atggaagagg
      781 gcgagatgtg tcgcgagaga cgggtcatcc ggttcgagac caagctccag gtctcgaccg
      841 ttttttttgg caaccatgac aaggcaactg ccccgcattg aaagccaggt gctgcttcgt
      901 cgctcgggta tcctgattcc cgcttccacg ggggagaggg aatcaaggcg cagtccggct
      961 ggtgagaccg gatcgcaggc attgcaccac cagaccgcat cctcgggcaa tggcaaacgg
     1021 agttgatgaa tggccaaggg tgaggcgaac tgcagcccgg ggataccctc aaaaaactgg
     1081 ccactctcga tctcgccaga gagttccaga agccgcaagg accggaagac ctgcgcccac
     1141 gaagactgcg ggagttcatg ctggaggatc tccctgaaga gaataccgta gcgatcgagc
     1201 aggatgcgca cccggtcgcg gatggactca tcggcttcaa gcagacttat cggttcatcg
     1261 cctcccttgg cggttttttt atggagaaca gtccacacgc caccctgggg cctggtccgg
     1321 ttccagcggg ctctcgcctg tccacggggc aggagggcca gcgaggcatt tgacggaagg
     1381 gcaaaatcat tggcgatggc ctggcgcagt gccacaaaat tatccgttgc gacacagcct
     1441 ttccaggcga gatcccagag tgtgccggcc agcgtatcgc tcgcaaggcc actgcccgct
     1501 acgagttcaa aaaaatcata gcgggccagc ggatccttga
//
LOCUS       DAOSHE010000483         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_85131_length_1533_cov_2.692152, whole genome shotgun sequence.
ACCESSION   DAOSHE010000483 DAOSHE010000000
VERSION     DAOSHE010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85131_length_1533_cov_2.692152"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLD82_10310"
     CDS             complement(<1..256)
                     /locus_tag="PLD82_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65815.1"
                     /translation="MSSEAPIGIGAGVRTLALISCVKDKLPYPAPAVEIYRGFFFASS
                     LRYARSLGPDAIYILSGKYGLLALEDPVEPYDLNLGEQPQA"
     gene            complement(253..1317)
                     /locus_tag="PLD82_10315"
     CDS             complement(253..1317)
                     /locus_tag="PLD82_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG65816.1"
                     /translation="MEYLVSFLAAAVVAGIPLLFATVGEIITERAGNLNLGVEGMMLM
                     GAVVGFLVGLTTKNAPLAILAAAMAGAFGAAIFAFLTITLRTNQVVTGLSLTIFGAGF
                     STMIGNELIGEVAPSAIKEFFKPFTQTLSFGGLDAVPAPWELDGLAALTPVGSLAHTG
                     HIPVLGDIPVIGRIFFQQDMFVYIGYAVAILAGIYLYRTRMGLNLRAVGENPGAADAS
                     SINVSLYKYVHILIGGALCGLGGAYLSLVYVPAWQENVTAGRGWIAIALVIFSGWNPY
                     KALSGAFLFGALSVLPLYFEELMRLIIGVPVQLTAFWRTVFDMMPYLVTIAVLFLSTL
                     KFRKNAPPAGLGLPFFREER"
     gene            complement(1317..>1533)
                     /locus_tag="PLD82_10320"
     CDS             complement(1317..>1533)
                     /locus_tag="PLD82_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65817.1"
                     /translation="PVILIVSVLFAALLEGGAYIQIAFNIPEAAANILQALILFFVLG
                     AEFLVRYRITTKGADVEALSAKSKEAG"
BASE COUNT          387 a          463 c          432 g          251 t
ORIGIN      
        1 aggcctgagg ttgttcccca agattgagat catacggctc gacaggatcc tccaaggcca
       61 agagcccgta cttccccgag agaatataga tggcatccgg cccgagactg cgggcatagc
      121 gcaaactcga agcgaaaaaa aagccccggt agatctcgac cgccggggct ggatacggaa
      181 gcttgtcctt gacgcaggaa atcagtgcca gtgtccgtac accggcacca attccgattg
      241 gagcttcgct gctcaccgtt cctcccggaa aaagggcagg ccaagcccgg ccggcggggc
      301 atttttccgg aatttgagcg ttgacaggaa gagaaccgcg atcgtgacga gatacggcat
      361 catgtcaaag accgttcgcc aaaaggccgt caactggacc gggacaccga taatcagacg
      421 catcagctcc tcgaaataaa ggggaaggac cgagagcgct ccaaagagga aggcgccgga
      481 gagggccttg tagggattcc atcccgagaa gatcaccagg gcaatcgcaa tccagccccg
      541 gccggccgtc acattctcct gccaggccgg gacatagacc agtgagagat aggccccgcc
      601 cagcccgcag agtgcaccac cgatcaggat gtgcacgtac ttgtacaggc tgacattgat
      661 gctggatgcg tccgcagctc cgggattctc cccaacggcc cgcaggttca accccatccg
      721 cgtccgatag agatagatcc ccgccaggat ggcaactgca tacccgatat agacgaacat
      781 gtcctgctgg aaaaagatcc tgcctatgac cgggatatcg cccagcaccg ggatatgccc
      841 ggtatgagcc aagcttccca cgggagtcag cgcggcgagt ccgtccagtt cccaaggagc
      901 cggaacggca tccagcccgc caaaggagag cgtctgcgtg aagggtttga aaaactcctt
      961 gatggcggaa ggcgccacct ctccgatcag ctcgttccct atcatcgtcg agaatccggc
     1021 accaaagatc gtcagggaca gtccggtcac gacctggttt gtcctgagag tgatcgtcaa
     1081 gaacgcgaag atggccgctc caaaagcgcc tgccatggct gccgccagaa tcgcgagggg
     1141 tgcgtttttg gtcgtcaacc cgaccaaaaa accgacaaca gcgcccatca acatcattcc
     1201 ctcgacaccg aggttcaaat tcccggcccg ctcggtgatg atctcgccaa cggtcgcaaa
     1261 gaggagcgga atccctgcca caacggccgc cgccaaaaac gaaacaagat attccatcaa
     1321 ccagcctcct tgctcttggc agagagggct tcaacatcgg cccctttggt cgtgatcctg
     1381 taccgaacga ggaattcggc gcccaggaca aaaaagagga tcagggcctg gaggatattg
     1441 gccgcggcct cgggaatatt gaatgcgatc tggatgtagg ccccgccctc aagaagcgct
     1501 gcaaagagca cggaaacaat caggatgacc ggc
//
LOCUS       DAOSHE010000484         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_85486_length_1529_cov_3.679104, whole genome shotgun sequence.
ACCESSION   DAOSHE010000484 DAOSHE010000000
VERSION     DAOSHE010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85486_length_1529_cov_3.679104"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLD82_10325"
     CDS             complement(<1..1128)
                     /locus_tag="PLD82_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65818.1"
                     /translation="MWCLKLRSLVLVTGLCLVVSLPVVSLNDVRLPFDEEDHFEDRGA
                     FGVLRLGRIVGEGRGLDSPGGNRIAVSASRTLWLARQRSLTEISFQRKTIQRYWFPES
                     CEPATISSIFVSKTAVWIGLSDGRVALRRFRDGTWEIHESGNRGPQRIMEVAGSILAF
                     SQESGGWVAYFHEAKRKFLPYFSLPGDLKAIPGSMKLYGRGWYLATDLGLFRIERPGT
                     EGMRWEMQGTRDGLSLMTIHDLAPLGNRLLLASSVSQYPRLPRHIRLTSYGSFVFDYF
                     QGVWKRQGEPGLSHFLAVNASNATIPANGLWVYAQAGDRARTVRGAEDEFFRVLPVGP
                     ETWLAAGARGLYHCGRVGQEYRAVRIVSLPGLWIDDLARDAE"
     gene            1221..>1529
                     /locus_tag="PLD82_10330"
     CDS             1221..>1529
                     /locus_tag="PLD82_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG65819.1"
                     /translation="MKGIIIAAGYGTRFLPCTKTIPKEMFPLVNKPAITFIIDEFLAS
                     GIKDILIVTSRRKKALDDYFDREVELESVFMREGKTAQLGIIAPPDANIYFTRQQTMR
                     G"
BASE COUNT          359 a          490 c          396 g          284 t
ORIGIN      
        1 ctcagcgtcc cgcgcaagat cgtcgatcca gaggcccggc agggacacga tccgtacagc
       61 tcgatactcc tgcccgaccc gtccacagtg gtagagacca cgcgccccgg ccgccaacca
      121 ggtctccggc ccgacaggaa ggaccctgaa gaattcgtct tcggctcccc ggactgtccg
      181 ggccctgtcc cccgcctgcg catagaccca cagcccgttg gccggaatgg ttgcattgga
      241 agcgttcact gcgagaaaat gggacaggcc cggctcgccc tgccgcttcc agacaccctg
      301 gaaataatcg aacacaaagc tgccatagct tgtcaatctg atgtgccggg gcaagcgggg
      361 atactgtgag accgaggaag ccaacaacaa gcggtttccc agcggtgcaa gatcatgaat
      421 cgtcatcagg gaaagaccgt cacgggttcc ctgcatctcc cagcgcatgc cctccgttcc
      481 cggcctttca atcctgaaca ggccgagatc ggttgccagg taccagcctc gtccatagag
      541 cttcatgctt cccggtatgg ccttgagatc tccgggcagt gagaagtacg ggagaaattt
      601 tcgttttgcc tcatgaaagt aggcgaccca ccccccggat tcttgtgaaa acgcgaggat
      661 actgcccgca acttccatga tccgctgggg accgcgattt ccgctctcgt ggatctccca
      721 ggtcccatcc cggaatctgc ggagtgccac ccgtccatcc gagagtccga tccaaaccgc
      781 cgttttcgag acaaaaatcg aagagatcgt cgccggttca caagactcgg gaaaccagta
      841 tcgctgaatc gttttgcgtt gaaacgatat ctcggtcaga ctccgctgtc tggccaacca
      901 gagggtgcgg gaagcactga cggcgatccg gttccctccc ggactgtcca acccccggcc
      961 ctcacccaca atccgaccca aacggaggac gccaaatgcc ccccggtctt caaagtggtc
     1021 ctcctcatca aacgggagcc ggacatcgtt caacgagacc acaggaaggg acacgaccag
     1081 acagagcccc gtcaccagaa caagggaacg aagtttcaga caccacatgc tcggaccacc
     1141 ttcccccggc tgcaaaatca gcactatact ttagtatcgt cggcctctgg gccatccatc
     1201 ttgaacgcgg ggagtcacca atgaagggaa tcattattgc agcaggatat ggcacacggt
     1261 ttttaccatg caccaagacc atcccgaagg agatgttccc tctggtcaac aagccggcca
     1321 tcaccttcat tattgacgag tttctcgcct cggggatcaa ggatatcctg attgtcacaa
     1381 gccgccgaaa aaaggccctc gatgactatt ttgaccggga ggtcgagctg gagagtgtct
     1441 ttatgcgcga ggggaaaacc gcccaacttg gaatcatcgc cccgcccgat gcaaacatct
     1501 atttcacacg gcagcaaacc atgcgcggc
//
LOCUS       DAOSHE010000485         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_85487_length_1529_cov_3.658752, whole genome shotgun sequence.
ACCESSION   DAOSHE010000485 DAOSHE010000000
VERSION     DAOSHE010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85487_length_1529_cov_3.658752"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PLD82_10335"
     CDS             <1..1299
                     /locus_tag="PLD82_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG65820.1"
                     /translation="RAKHFYSIYRKMQTKNKSFGEIHDLMAVRIICERIPDCYQILGI
                     IHSLYRPLYDRFKDYIALPKSNMYSSLHTTIVGPDGKLLEIQIRTREMDSIAEEGAAS
                     HLLYKAGHTDEMRDMASTLPWLAKLKNWQDSLTDPRQFMQELTGEMLQDEIYVFTPKG
                     DVIRLVTDATPLDFAYAIHTDVGQHTIGARVNGKMVPLKTALKNCDVVEIITSRTAHP
                     TSAWLRVARSSRARHKIRVWLKNAMLDTEQLDLEVRTRDPAQGNDPKSRRRRPVPGSG
                     AQPQPEVEIGTPGIVVDGERNLLFEFARCCNPQPGDPILGYLSRGRGISIHRHDCPNI
                     PNLLGEPDRIVYPAWRARERVFRKTISLEASDDPWILPDLVALIAAHNAGIQSVRRLP
                     LPDGQTRIDLEVECATEAAWNLVQSSLDQSKRFRTLGDST"
BASE COUNT          343 a          503 c          411 g          272 t
ORIGIN      
        1 cgggccaagc atttttacag catctaccgc aagatgcaga ccaagaacaa gtcgtttggc
       61 gagatccatg acctgatggc cgtccgcatc atctgcgaac ggattccgga ctgctaccag
      121 atcctgggga tcatccactc cctctatcga ccgctctacg accgcttcaa ggactacatc
      181 gccctcccca agtccaacat gtactcctcg ttgcacacca ccatcgtcgg gcctgacggg
      241 aaactcctcg agatccagat ccggacccgg gagatggaca gcatcgccga ggaaggcgcc
      301 gccagtcacc tcctctacaa ggccggccat acggacgaga tgcgggatat ggccagcacc
      361 cttccctggc tggcaaaact gaaaaactgg caggactcgc tgaccgatcc ccgccagttc
      421 atgcaggagc tgaccggcga gatgctccag gacgagatct acgtcttcac gcccaagggc
      481 gacgtcatcc gcctggtcac cgatgcgacc ccccttgatt ttgcctatgc catccacacc
      541 gatgtcgggc agcacacgat cggggcacgg gtcaacggga aaatggttcc cctcaagacc
      601 gccctcaaga attgtgatgt cgtcgagatc ataacgtccc ggaccgcaca cccgaccagt
      661 gcctggctcc gcgtggcccg ctcatcccgc gcccggcaca aaatccgcgt ctggctgaaa
      721 aacgccatgc tggataccga gcagcttgat ctggaagtgc ggaccaggga cccggcgcag
      781 ggaaatgacc cgaaaagccg gcggcggcgg ccggtgccgg gatccggcgc ccaaccccaa
      841 cccgaagttg agatcgggac accgggcatc gtggttgacg gtgaacggaa tctgctcttc
      901 gagttcgccc ggtgctgcaa cccgcagccg ggtgacccga tcctgggcta tctctcccgc
      961 gggagaggaa tcagcatcca ccgccatgac tgtcccaaca tccccaacct cttgggagaa
     1021 ccggaccgga tcgtgtatcc cgcatggcgg gcccgggagc gggtcttcag gaaaacgatc
     1081 agcctggaag ccagtgatga cccgtggatc ctgcccgacc tggtggccct gatcgcggcc
     1141 cacaacgcgg gaatccagtc cgtccgccgt ctccccctcc cggacgggca aacccggatc
     1201 gatctcgagg tggaatgcgc caccgaagca gcatggaacc tggtccagag ttctctcgac
     1261 cagagcaaac gcttccggac acttggcgat agtacttgaa aaaaccactc gccaattggt
     1321 atatttgtca ttattccaca gaaatcggat accatggcag ctggaaccag catactgttg
     1381 ataacgacag aaaatcaggt cacaaccgga attgcacgtc tgctgcgttc cctgcatgat
     1441 gcgtccctgc gccttgcaag ctgccacccc gatgaaatcg gcgattacga tttttcggca
     1501 ctctcgctga ttctccttga taacgaaat
//
LOCUS       DAOSHE010000486         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_85768_length_1526_cov_2.465670, whole genome shotgun sequence.
ACCESSION   DAOSHE010000486 DAOSHE010000000
VERSION     DAOSHE010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85768_length_1526_cov_2.465670"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PLD82_10340"
     CDS             <1..167
                     /locus_tag="PLD82_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65821.1"
                     /translation="IDSGLARKHEGTGLGLSICKRLVEMMGGTIDVVSEFGKGSQFTV
                     CLPLGGSGDA"
     gene            160..540
                     /locus_tag="PLD82_10345"
     CDS             160..540
                     /locus_tag="PLD82_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG65822.1"
                     /translation="MPERLLVIEDNEQNHYLMRFLLEKHGFAVEVAETGRAGVKAALL
                     HPPRAILLDIQLPEMDGYAVAEALLAEESLKHIPIIAVTSYAMAGDRERILAAGATGY
                     IEKPINPDTFVAQIRGYLDGARDA"
     gene            688..1149
                     /locus_tag="PLD82_10350"
     CDS             688..1149
                     /locus_tag="PLD82_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG65823.1"
                     /translation="MAVPIFSFLATLETDIADFYRRLKSVSRLVHSDKLFDFMASHSS
                     GHSQEILGLVSRHARPELDQAFLREVHEQIKVSLMAEIRNAPDLSEAVEKIARTEELV
                     GKMYQMIAAHYRRLAEHCTGVAAELERIAGEEFQHRDMVLKERERYGGEGG"
     gene            complement(1217..>1526)
                     /locus_tag="PLD82_10355"
     CDS             complement(1217..>1526)
                     /locus_tag="PLD82_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65824.1"
                     /translation="TQADVGQVGLVHLRGHNEAVGEDEELIGAGHGLAGLDLAVDDLA
                     AGRAISLEAVTAAARPPLPIWLFAKAGNMMVRKKIRSLRKSGTDPDARPFAEERIGQD
                     "
BASE COUNT          297 a          406 c          501 g          322 t
ORIGIN      
        1 agatcgacag cggtttggca cgcaagcacg agggcaccgg gctcggtctt tcgatctgca
       61 agcggcttgt cgagatgatg ggcgggacga ttgacgtggt cagtgaattc ggcaagggaa
      121 gccagtttac ggtctgtctt cccttgggag ggagcgggga tgcctgaacg acttcttgtg
      181 attgaagaca atgagcagaa ccactatctg atgcggtttt tgctcgagaa gcacggattt
      241 gcagtcgagg ttgccgagac gggtcgggcc ggggtcaagg ccgcgcttct ccatccgccc
      301 agggccatcc ttctcgatat ccagcttccc gagatggacg gctatgcggt ggccgaggcg
      361 ctcctggccg aggagagtct caagcacata ccgatcatcg cggttacttc gtatgccatg
      421 gccggagacc gggaacggat tctggctgcg ggggcgacgg ggtatatcga gaaaccgatc
      481 aacccggata cctttgtagc ccagatccgg ggctatctgg atggagcccg ggatgcctga
      541 ctcggggcgt gcgtttatgg caggggtcgg ccagcacaat cgctctctgg agcatgtgcc
      601 ggatccggcc tgagtgtgtt caacaaaaac cggaccaata caggatcgcc tgttccggta
      661 cagattctga gcaaaaggag tcgaggcatg gctgttccca tattcagttt tctcgcgaca
      721 cttgaaaccg atattgcgga tttttaccgc aggctgaaat cggtgtcgcg cctggtgcat
      781 tcagacaaac tctttgattt tatggccagt cattccagcg gtcattcgca ggagattttg
      841 gggctggtgt ctcgtcatgc cagaccggag ctggaccagg catttttgcg ggaagtccac
      901 gagcagatca aagtctcgtt gatggccgag atccggaacg cccccgatct ctccgaggct
      961 gtcgagaaga tcgcccggac cgaggagctg gttggcaaga tgtaccagat gatcgcggcc
     1021 cattaccgga ggctggcgga gcattgtacc ggtgtcgccg ccgagctcga gcggattgcc
     1081 ggtgaggaat tccagcaccg ggacatggtt ttgaaggaga gggagcggta cggcggcgag
     1141 ggtggataaa agggttcccc cgcaaacagg atgcatccgg attatgaaac ggcactggat
     1201 tggctgttcc gggttgtcag tcttgtccta tccgttcttc agcaaacggc cgggcatccg
     1261 ggtcggttcc acttttgcgc agtgagcgga tttttttccg gaccatcatg ttgccggcct
     1321 tggcgaagag ccagatggga aggggtggtc tggcggcggc tgtgactgct tcgagggata
     1381 tggcccggcc ggcggccaga tcatcaaccg caaggtcgag cccggccaga ccgtgtccgg
     1441 cgccgatgag ctcctcatcc tcgccgaccg cctcattgtg gccgcgcagg tggacgagac
     1501 cgacctggcc cacatccgcc tgggtc
//
LOCUS       DAOSHE010000487         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_86013_length_1523_cov_3.483651, whole genome shotgun sequence.
ACCESSION   DAOSHE010000487 DAOSHE010000000
VERSION     DAOSHE010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86013_length_1523_cov_3.483651"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLD82_10360"
     CDS             complement(<1..274)
                     /locus_tag="PLD82_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG65825.1"
                     /translation="MDNEASDAEVLAHVSQGDSAMFRVIVDRHARRLHAWAWRYTSSD
                     EDAEELVQETLLRAFRSADKYDPSRPFVPWLFTILANLCHNWHKSRR"
     gene            421..>1523
                     /locus_tag="PLD82_10365"
     CDS             421..>1523
                     /locus_tag="PLD82_10365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG65826.1"
                     /translation="MSEIELGPDQYRVEEEARPQAEPVRGDCLLDRLDMILVFMIGFG
                     ALFLLELLMGGAEKPGVLIALGGNYAPFVERGEIWRLLTANFLHGNMMHIIVNGYSFW
                     ILGRFVERVYGRSRLTLVFVLTATVSALASWLANASLSVGASGGITGFLGLLAAFTLH
                     YRNRLDPRFRRSFLSNLAMIIGINVVIGVSYSGIDHWAHLGGFVAGLFLGFLVRPIVL
                     FGGGTHPRRIRLAVRLTGVLLVAAFAAQLYALVFSVIIPEKGPARMLSDAGGRFSLAY
                     PAFFSVEQGRDEERGTIRIGNNLDRTIVLEVMQREYALRVMEGLRASGIDTQEGLVNG
                     YRVIRVHGTVTVQGRRVLLRMALVEAPADSNAAG"
BASE COUNT          255 a          428 c          502 g          338 t
ORIGIN      
        1 cgcgccggct cttgtgccag ttgtggcaga gattggcgag aatggtaaag agccagggca
       61 cgaagggccg ggacggatcg tatttgtcgg ccgagcggaa ggcccggagc agggtttcct
      121 ggaccagctc ttcggcatcc tcatccgatg aggtgtagcg ccaggcccag gcgtggagtc
      181 tgcgtgcatg acgatcgacg atgacacgga acatggcaga gtccccctga ctgacatgcg
      241 caagaacctc ggcatcactt gcttcgttgt ccaccggcgc catcccgttt gattgagtct
      301 ctgcttccat atcaaactac agagcgggag gcttaccggc aatacgatca atacccggcg
      361 gaaccctcca gagttcaggg ggattttgtt atttttgtgt gggatgcgga gggaggagcg
      421 atgagcgaga ttgaactggg cccggatcag taccgggtgg aagaggaagc ccggcctcaa
      481 gcagaaccgg tcaggggtga ctgcctcctg gaccggctgg acatgattct cgtattcatg
      541 atcgggtttg gagccttgtt tctccttgaa ctcctcatgg gcggggccga aaagcccggg
      601 gtcttgattg cgctgggtgg caattatgct ccctttgtcg agcggggcga gatctggcgt
      661 cttctgacag ccaatttctt gcacggtaac atgatgcaca tcattgtcaa cgggtacagt
      721 ttctggatcc tgggccgctt cgtcgaacgg gtctacgggc ggagccggtt gaccctggtc
      781 tttgtcctga cagccacagt ctcggccctg gccagctggc tggccaatgc ttcgctctcg
      841 gtcggtgcat cgggagggat cacggggttt ttggggctcc tggcggcctt taccctgcat
      901 taccgcaaca ggcttgaccc ccgcttcaga cggagctttc tctccaatct ggccatgatc
      961 atcggcatca atgtcgtgat cggggtttca tacagcggga tcgatcactg ggcccacctg
     1021 ggcgggttcg tggccgggct cttcctcggg tttcttgtgc gcccgatcgt cctcttcgga
     1081 ggcgggaccc atccccggcg gatccgcctg gctgtgcgcc tgaccggggt acttctggtg
     1141 gccgcctttg cggcccagct gtatgccctt gtgttttcgg tcatcattcc cgaaaagggt
     1201 cctgcccgga tgttgagcga tgccgggggg cgcttcagcc tggcctaccc ggccttcttc
     1261 tccgttgagc aggggcggga tgaagagcgg gggacaatcc ggatcgggaa caatcttgac
     1321 cggacgattg ttctcgaggt gatgcagcgt gaatacgcgc tgagagtcat ggagggcttg
     1381 cgggcatccg ggatcgatac acaggagggc cttgtcaacg ggtatcgcgt gattcgtgtc
     1441 cacgggaccg tcaccgtcca gggccgccgg gtcctcctgc ggatggccct ggtcgaggcg
     1501 cctgccgact cgaacgccgc ggg
//
LOCUS       DAOSHE010000488         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_86357_length_1519_cov_3.347678, whole genome shotgun sequence.
ACCESSION   DAOSHE010000488 DAOSHE010000000
VERSION     DAOSHE010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86357_length_1519_cov_3.347678"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PLD82_10370"
     CDS             <1..1126
                     /locus_tag="PLD82_10370"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOG65827.1"
                     /translation="PGEEFSGIDNHIWSHAWSLSGGKNQGDGTGIRTYDGKTINNYTI
                     QPEYNERAGDSTIGVFCHEFIHKLGLPDLYVTTYSTQGVGQFSIMAGGAWLGPGRDGS
                     RPAPMLAWERAALDWVDTVSAASIGTAVSIKEPDSSAMQQLVWGTSSVPTDSSAPGDS
                     VLLLAACVVIVRRRLMRPAHRMGVLLLLVLLPGFWACSDDEPEIPRPSAQYRVCIDDI
                     ETSRKAIMIPLGDRAGQQFYLVENKVVKAGTWTEYLPGSGLLISKIHQTVYDTHNNMN
                     SVNDGPARIHGVAIVEADNDAMLWKGQDLEAGTTDYTDSGRQEDLFASRSFTAETSPA
                     SCFYTASAAPWSISGTALSAAGLSSISAAGPTMTCICHIP"
     gene            1169..>1519
                     /locus_tag="PLD82_10375"
     CDS             1169..>1519
                     /locus_tag="PLD82_10375"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HOG65828.1"
                     /translation="MNHPGPVWNRILSLLCLIILTSCSNGVKQEGDLLPKRDSADEIA
                     CDYRETLKKWQESAYRHYTMYFRYGAFSPVAEDWEIEIKDGRVVKRMQNGQPVPAERP
                     TMTNMRMERLFELAA"
BASE COUNT          348 a          466 c          409 g          296 t
ORIGIN      
        1 cccgggtgaa gagttcagtg gcatcgacaa ccacatctgg tcccacgcct ggtctctctc
       61 gggcgggaaa aaccagggag atggcacggg catccgcacc tatgatggca aaactatcaa
      121 caattacacc attcagccgg agtacaacga acgcgccggt gactccacca tcggggtctt
      181 ctgccacgag ttcattcaca aactcggcct cccggatctc tacgtcacca cctacagcac
      241 ccagggtgtc ggccagttct cgatcatggc cggcggagcc tggctcggtc cgggcaggga
      301 cggttcgcgt ccggccccca tgttggcctg ggagcgggct gcactggatt gggtagacac
      361 cgtttccgca gcctcgatcg gaaccgccgt ttccatcaag gaaccggatt catcagccat
      421 gcaacagctt gtatggggaa catcctcagt cccgaccgat tcgtcagcgc ccggggatag
      481 tgttttgctc ctggcggcct gcgttgtcat tgtcagacga cgcctgatgc gcccggcaca
      541 ccggatgggc gtgctcctcc tgctggttct cctccccggc ttttgggcct gctccgatga
      601 tgaaccggag ataccccggc cttcagcaca atatcgggtg tgcatcgatg atatcgagac
      661 atcccgaaag gccatcatga ttccgctcgg agaccgggcc ggccagcaat tctatctcgt
      721 tgaaaacaag gtggtcaagg ccgggacatg gaccgaatac ctgccgggaa gcgggctctt
      781 gatcagcaag atccaccaaa ccgtgtacga cacgcacaac aatatgaatt ccgtcaatga
      841 tggtccagca cggatccacg gggtggccat tgtcgaagcg gacaacgatg ccatgctctg
      901 gaaaggccag gatcttgaag ccggaaccac ggactacaca gacagcgggc gacaggagga
      961 tctcttcgca tcacgcagct ttactgccga gaccagcccg gcaagttgtt tctacacggc
     1021 cagcgccgca ccatggtcca tcagcgggac agcgctttcc gcagccggtc tgtcttcaat
     1081 cagtgccgcc ggccccacca tgacctgtat ctgtcatatt ccataaacgg attgcgttta
     1141 tatgcccttc ataaaggaga agcaaaacat gaatcacccc ggtccggtct ggaacagaat
     1201 cctgtccctc ctctgcctga tcatcctgac ctcctgctcc aatggagtca aacaggaagg
     1261 cgacctcctc ccaaagaggg attccgctga cgagatcgca tgcgattatc gcgagactct
     1321 caaaaaatgg caggaatccg cataccgcca ttacaccatg tacttccggt atggcgcttt
     1381 cagtccggtt gccgaagact gggaaatcga gatcaaggac gggcgtgttg tcaaaagaat
     1441 gcaaaacggc cagcctgtgc ccgcagaacg cccgaccatg acaaatatga gaatggaacg
     1501 tctcttcgaa ctggcggcc
//
LOCUS       DAOSHE010000489         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_86367_length_1519_cov_3.043716, whole genome shotgun sequence.
ACCESSION   DAOSHE010000489 DAOSHE010000000
VERSION     DAOSHE010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86367_length_1519_cov_3.043716"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLD82_10380"
     CDS             complement(<1..870)
                     /locus_tag="PLD82_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65829.1"
                     /translation="MNERGQTLLIRFVLLFPLLSLCILGVYRLFLPLVAPETVDQARH
                     LVDAIVPAAAIGLALSAALSFAALSHGSQEQSSDLTPIRLAFGWMLFFALALMAFASF
                     GIPAIHKSLSLDATAPTAPRPVKPDPPVNTLKEGLKHYYSAKYEAAIDRIQEFTQSMP
                     GHTIAADYLASARAALARQEQIKSTRAGDQTATFRKGYENLLEGNYLAAIMEFERLLR
                     FQPNHSLAKKHLADARARLKAEQSGTEGDTRTTGLKARLERTVSLGIKRYESGDLVGA
                     LSLLGFGASRADGL"
     gene            complement(867..>1519)
                     /locus_tag="PLD82_10385"
     CDS             complement(867..>1519)
                     /locus_tag="PLD82_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG65830.1"
                     /translation="EQSLQRQLADSPSSLIAGSILFLPQFKPGAGLISNTYLCSSGRV
                     FSGGTLRVEDWGLTNAVLLSSNARHVPWVPLSKSIRDILPHAHPSLVSASFDARLSSI
                     VRWPVTGSFSGNTLMPESAAWLIGLALLACGAGLLINRARISLFHRAGAYLYFLSLFV
                     VPVWLFHIWWLNRPAPGGLIPEAHVFIRSGGFLFAGLAIFVLGFWRRKRLERGALP"
BASE COUNT          320 a          400 c          540 g          259 t
ORIGIN      
        1 cagaccgtcc gcgcgtgacg caccgaagcc gaggagcgag agcgcgccga caagatcgcc
       61 tgattcatag cgcttgattc cgagggaaac ggtccgctca agacgggcct tgagcccggt
      121 tgtccgggta tcgccctcgg tgccgctctg ctcggctttc agcctggccc tggcgtcagc
      181 cagatgcttt tttgccagac tgtggttggg ttgaaaccgg agcaggcgtt cgaattccat
      241 gatggctgcc agataattcc cttcaagaag gttctcgtag cctttgcgga aagttgccgt
      301 ctggtcgcca gcccgggtgc ttttgatctg ctcctggcgg gccagtgcgg cccgggcgct
      361 ggccaggtag tctgccgcga tcgtatgccc cggcatgctc tgtgtgaatt cctggatccg
      421 gtcgatggcc gcctcgtatt tggcggagta gtagtgcttg agtccttcct tgagggtgtt
      481 gacgggcgga tccggcttga cgggccgggg ggccgtgggt gctgttgcgt ccagcgacag
      541 agatttatgg atggccggga tgccgaacga ggcaaaggcc atgagggcca gggcgaaaaa
      601 gagcatccag ccaaaggcca ggcggatcgg ggtcagatcg ctggactgct cttgtgagcc
      661 atgggaaaga gcggcaaagg agagcgcggc cgagagcgcg agcccgatgg cggcagccgg
      721 gacaatggcg tcgacaagat ggcgggcctg gtcgaccgtc tctggcgcaa ccagggggag
      781 gaagagtctg tatacgccga ggatgcacag ggaaaggagg ggaaagagga gtacgaaccg
      841 gatcaacagg gtttggccgc gttcattcat gggagggcac ctctctcgag acgctttcgc
      901 cgccaaaatc ccaggacaaa gatggccaga ccggcgaaca aaaatccacc gctccggatg
      961 aagacgtgcg cttcagggat cagtccgccc ggggccggcc ggttgagcca ccaaatgtga
     1021 aagagccaga ctggaaccac aaaaagggag agaaaataca ggtaggcccc ggcccggtgg
     1081 aacagcgaga tacgggcgcg attgatcagg agtccggctc cgcaggccag cagtgccagc
     1141 ccgatgagcc aggcggcact ctcgggcatg agggtgttcc ccgaaaaaga accggtgacg
     1201 ggccagcgaa ctatcgagga caggcgcgca tcaaaggatg ccgagacaag ggacggatgg
     1261 gcgtgcggga ggatgtcgcg gatggatttg gataacggga cccagggaac gtggcgggcg
     1321 ttgctggaca ggagaacggc attggtcaga ccccagtctt ccacccgaag ggtcccgccg
     1381 gagaagaccc ggcccgatga acagaggtac gtattgctga tgaggcctgc ccccggcttg
     1441 aattgcggga ggaagagtat ggatccggcg atgaggcttg acggggaatc tgccagttgt
     1501 cgctggagtg attgttctt
//
LOCUS       DAOSHE010000490         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_86779_length_1514_cov_4.394106, whole genome shotgun sequence.
ACCESSION   DAOSHE010000490 DAOSHE010000000
VERSION     DAOSHE010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86779_length_1514_cov_4.394106"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLD82_10390"
     CDS             <1..1153
                     /locus_tag="PLD82_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG65831.1"
                     /translation="AESANRAKSEFLANMSHEIRTPLNAVIGFTDLLSKTALDETQAG
                     YVRHANTAGRALLGIINDILDLSKIEAGKLDLEILPADPSALAQQAMDIVRYQAEAKT
                     LSLDLRLAPDIPRAIMTDPIRLQQILINILGNAVKFTPAGSVALEVSVPSARQLEFAV
                     RDTGIGISPEIREKLFQPFSQADSSTTRRFGGTGLGLAISRLLTEKMGGEITLESTAG
                     TGSCFRIRIPLVTPPSSPAGPETPDRIQQGNPELDQAKNPLRPKRVLIAEDVPVNMLL
                     TTVMVRGILQESLIFEATNGSETVEAWKKEQPDLILMDVQMPTMDGLEATRIIRDLET
                     VHGGHTPIIALSAGTFSGEKERCLESGMDDFLAKPVLGDDLARTLQRWL"
     gene            1164..>1514
                     /locus_tag="PLD82_10395"
     CDS             1164..>1514
                     /locus_tag="PLD82_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG65832.1"
                     /translation="MQNLRAILLAVLVLFALPVSLPAKDRLIIGIDRSAAPMEFERDG
                     KPAGFNVDIMRAIADLGGYQPEFRFLDWTNVLESVRNRTIDIMFAMRTSEWEKIYLFG
                     TPFLEISVRIFVRED"
BASE COUNT          348 a          483 c          380 g          303 t
ORIGIN      
        1 cgccgaatcc gccaacaggg caaagtccga gttcctggcc aacatgagcc acgagatccg
       61 gacaccactc aatgctgtca tcggattcac cgatctgctc tcgaagaccg cgctcgacga
      121 aacgcaggct gggtacgtcc gccatgccaa caccgccggt cgggctctcc tcgggatcat
      181 caatgacatt ctcgacctct ctaagatcga ggctggaaaa ctcgatctcg agatactccc
      241 tgccgatccg tccgcgcttg cacagcaagc catggacatt gtccgctatc aagccgaggc
      301 aaagacactt tcattggacc tgcgcctcgc accagacatc ccccgcgcca tcatgactga
      361 cccgatccgt ctccagcaaa tcctgatcaa catcttggga aatgccgtca aattcacccc
      421 ggcgggatcg gtcgccctgg aagtctctgt cccctccgcc cggcaactcg aattcgccgt
      481 ccgggatacg ggaatcggca tcagtcccga aattcgtgaa aaactctttc agcccttttc
      541 gcaggccgat tcatcaacga ctcgccgctt tggcggaacc ggccttggtc tcgcaatctc
      601 ccgcctttta accgaaaaaa tgggcggaga gatcaccctg gaaagtacgg caggcacagg
      661 gtcctgtttc cggatccgga ttcctctcgt cactccaccc tcatctcccg caggtccgga
      721 gactccggac cgtatccagc agggcaaccc agaattggac caggcaaaga atcccctgag
      781 acccaaacgg gtcctgatcg ccgaagacgt ccccgtcaat atgctcctga caaccgtcat
      841 ggtgcgtggc atacttcagg agagtctgat ctttgaagcc acaaacggga gcgaaacggt
      901 ggaagcctgg aagaaggagc agcctgatct gattctgatg gatgtccaga tgccaaccat
      961 ggacgggctt gaagcaacac gcatcatcag ggatctggaa acggtgcatg gcggccatac
     1021 tccgatcatc gcactcagcg ccggaacctt cagtggcgaa aaagaacgct gtctcgaatc
     1081 cggcatggac gacttcctcg caaaacctgt ccttggggat gaccttgccc gcaccctcca
     1141 gcgctggctg taaggaaccg cccatgcaaa acctgcgagc catactcctg gcagtcttgg
     1201 tactctttgc gctcccggtc tccctcccgg ccaaagaccg gctcatcatc ggaatcgatc
     1261 gttcggcggc tccgatggag ttcgagcggg atggcaaacc tgccggtttc aatgttgaca
     1321 tcatgcgggc catcgcagat ctcggcggat accaacccga attccggttt ctggactgga
     1381 ccaatgttct ggaatccgtc cgcaaccgga ccatcgacat catgttcgcc atgagaacgt
     1441 ccgaatggga aaaaatttat ctcttcggaa ctccctttct cgaaatctca gtccggattt
     1501 tcgtcaggga agat
//
LOCUS       DAOSHE010000491         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_87840_length_1502_cov_3.297167, whole genome shotgun sequence.
ACCESSION   DAOSHE010000491 DAOSHE010000000
VERSION     DAOSHE010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87840_length_1502_cov_3.297167"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="PLD82_10400"
     CDS             <1..1366
                     /locus_tag="PLD82_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG65833.1"
                     /translation="NDEAFGASESALLDPVSIRQAVAQKTATAADTILYGSEKLPVIT
                     VYAPILHNGEPAAVLCLTADIKQHITSFQAAWKERYFRITMCNSKGIVLISSLADTIT
                     VLDASKEEGVKETSGPLLRGETGFGSYVYRGYTIIGGYAPVNVMDWRVAAGQPAAVYE
                     TAIRKVRTLLLAVSGGILLLSILIFSLVISRIVRPLRSLVNTLGASVHQVRLAASQVN
                     SASQALASATSQQAASLEETSAALEELTGMTRQNADNALSANKLMLRADELMRLADEK
                     MSQLVTAMEEITQASQDTKKIIKTIDEIAFQTNLLSLNAAVEAARAGDAGAGFAVVAD
                     EVRSLSQRSAEAARNTTGMIETSVQRINEGGTMMQETSIAYTEVQSTISQVKHLIAEI
                     SEASKEQAIGLDSISHAVHQMDLVVQNNAASSEETAASSNETARETDNIERDITSLDA
                     MVNG"
BASE COUNT          336 a          491 c          399 g          276 t
ORIGIN      
        1 caatgacgaa gcctttggcg cctccgagag cgccctcctt gatccggtga gcatccgcca
       61 ggcagtcgca caaaaaacgg ctacagcggc cgataccatc ctctacggga gcgagaaact
      121 tcccgtcatc accgtgtatg ctcccatcct gcacaatggc gaacccgctg ccgttctctg
      181 tctgaccgcc gacatcaaac agcacatcac gtctttccag gccgcctgga aggagcgcta
      241 cttccggata accatgtgca attccaaagg gattgtgctg atcagcagcc tggcggacac
      301 tatcaccgtc ctggatgcgt ccaaagaaga gggtgtcaaa gaaacctccg gcccccttct
      361 ccggggagag accggattcg gctcctatgt ctaccgcggc tataccatca tcggcggcta
      421 cgccccggtc aacgtcatgg actggcgtgt cgccgccggt caaccggccg ctgtctatga
      481 gaccgcgatc cgaaaagtcc gcaccctcct gctggccgtt tccggcggga ttctccttct
      541 gtccattttg atcttctctc tcgtgatctc gcggattgtc cgccccctgc gttccttggt
      601 caacaccctc ggggccagcg tccatcaagt ccgactggcg gcatcccaag tcaacagcgc
      661 cagccaagcc ttggcgagtg ccacatccca gcaggccgca tctctcgagg aaacctcggc
      721 cgccctcgaa gaactgaccg gcatgacgcg acagaatgcc gacaacgcac tctcggccaa
      781 caaactcatg ctgcgtgccg acgagttgat gcgtttggcg gatgaaaaaa tgagccaact
      841 ggtcacggcc atggaagaaa tcacccaggc ttcccaggat accaaaaaaa tcatcaagac
      901 catcgacgag atcgccttcc agacaaatct tctctcactg aatgccgctg tcgaagcagc
      961 ccgtgccggg gacgccggcg ctggctttgc ggtggtcgct gacgaagtcc gttccctctc
     1021 gcaacgcagc gccgaggcag cccgcaacac caccgggatg attgagacct cggtacagcg
     1081 catcaacgag ggtggaacga tgatgcagga gaccagcatc gcctataccg aggtccagtc
     1141 gaccatcagt caggtcaagc acctgatcgc agagatctcc gaagcgtcca aggaacaggc
     1201 catcggactg gacagcatta gccatgccgt ccaccagatg gacctggttg tccagaacaa
     1261 cgctgccagt tctgaagaaa cggccgcatc gtccaacgaa accgcccggg agacggacaa
     1321 tatcgaacgg gacatcacaa gtctggacgc catggtcaac ggttgaaaac ctgatgcaga
     1381 ctgccttgtc cagctttctt gtatcgagga tctgttttct gcccatcggg gtgaggaccc
     1441 gtcctcagcc catcggggtg aggacccgtc ctcagcccat cggggtgagg acccgtcctc
     1501 ag
//
LOCUS       DAOSHE010000492         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1735
            NODE_88003_length_1500_cov_3.229066, whole genome shotgun sequence.
ACCESSION   DAOSHE010000492 DAOSHE010000000
VERSION     DAOSHE010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863264
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:41:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,049
            Genes (coding)                    :: 2,031
            CDSs (with protein)               :: 2,031
            Genes (RNA)                       :: 33
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88003_length_1500_cov_3.229066"
                     /isolate="AMR_MDS_1735"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLD82_10405"
     CDS             <1..606
                     /locus_tag="PLD82_10405"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HOG65834.1"
                     /translation="PVGGFTRERLVTIAGRVGGDAKTVRVIHNGTGFVVPVANGRFKQ
                     ALLAARGDNLCIVRTLDNKQEQRVSFFAEVPKVDLKVFLYWDVVPSEYIDLWVHEPSG
                     EICKWNNRTTKAGGTLFDLYNGDIGRGPQYYALDVAPPGEYGFSVHYYTKQGKEPRRC
                     HVVFVIHEGTNDERREEFTFVLPRQHSEHVVKRIMIEGRAD"
     gene            complement(581..>1500)
                     /locus_tag="PLD82_10410"
     CDS             complement(581..>1500)
                     /locus_tag="PLD82_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG65835.1"
                     /translation="TGGTLINPGIPIPPEITTITGIDDTMVSGAPRESAVIDDLLSSL
                     ARADLVGGHNFTDFDSKFLEAFCARCGKRPPALPHFDTLHLARRYLEGQPNNKQPTLL
                     AWLGWQGEVFHRAVDDTRGCAFVLRAALRSAAGVHAYIEEVLPFVFLAEGHLAPGTEN
                     TGTARHTQIAGGLFSGPVQTRSGLETVPPKKIFCTRLAYEDQSVPDSINAMGQLVHDP
                     VPDSIVILLENLPGNAARRTALPEDGLAIDLPGLTWIITAPPTIWDDARIRPDARYDC
                     IIRIEGAPVEGTATATLLDLRLNPLALRS"
BASE COUNT          295 a          397 c          509 g          299 t
ORIGIN      
        1 ccggttggcg gttttacccg cgaacgcctt gtgaccattg caggcagggt gggcggggat
       61 gccaagaccg tgcgggtcat ccataacggg acgggctttg tcgtaccggt cgccaacggg
      121 cggttcaagc aggcgcttct ggccgcgaga ggagacaatc tctgcatagt gcggacgctg
      181 gacaacaaac aggagcaacg ggtttccttt tttgccgaag tccccaaagt ggatctcaag
      241 gtcttcctct attgggatgt cgtgccaagc gagtatatcg atctctgggt gcatgagcct
      301 tcaggggaga tctgcaaatg gaacaaccgg acgaccaagg ccggggggac actcttcgat
      361 ctctacaacg gcgatatcgg ccgggggccc cagtattacg ccctggatgt tgctcccccg
      421 ggagaatacg gattctcggt gcactattat accaagcagg gcaaggagcc gcgccgctgc
      481 catgttgttt ttgtaatcca cgaaggaacc aatgacgaac ggcgagagga atttaccttt
      541 gtcctgccgc gtcagcacag cgagcacgtc gtcaagcgga tcatgatcga agggcgagcg
      601 gattgagacg gagatccagg agggttgcgg tggctgttcc ttcgaccggg gcgccctcga
      661 tgcggatgat gcagtcgtag cgggcatccg ggcggattcg cgcatcgtcc cagatcgtgg
      721 gcggggcggt gatgatccag gtcaggccgg ggaggtcgat ggcgagaccg tcttcaggta
      781 aggctgtacg tcttgctgcg ttgccgggca ggttctccag aaggatgacg atgctgtccg
      841 gcacaggatc atggaccaac tggcccatgg cgttgatgga gtccggtacg gattggtctt
      901 cgtatgccag tctggtgcaa aagatttttt ttgggggaac agtctccagt cctgagcggg
      961 tttgaacggg tcccgaaaag agtcctcccg cgatctgtgt atgccgcgca gtgccagtgt
     1021 tttccgtccc gggtgcgagg tgcccctcgg cgagaaacac aaagggcagg acttcctcga
     1081 tgtacgcatg cacaccagcc gcgctgcgca gggcggcgcg caagacaaag gcgcatcctc
     1141 gggtgtcatc caccgcccgg tgaaagacct cgccctgcca gccgagccag gccaggagag
     1201 tgggctgctt gttgttgggc tgaccctcga ggtagcgtct cgccaggtgg agcgtgtcaa
     1261 aatggggaag agcggggggg cgttttccgc agcgcgcaca gaaggcttca agaaatttgc
     1321 tgtcaaaatc cgtaaagttg tgcccgccga ccagatccgc ccgagccagg cttgagagga
     1381 gatcgtcgat gacagcggat tcccgggggg cgccagacac catggtgtcg tcgattccgg
     1441 tgatggtcgt gatctcgggg gggatcggga tgccgggatt gatcagggta ccgccggttt
//
